LOCUS       DAOPOE010000001       111821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_184_length_111821_cov_6.307902, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000001 DAOPOE010000000
VERSION     DAOPOE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 111821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 111821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111821
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_111821_cov_6.307902"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..3920
                     /locus_tag="PKM94_00005"
     CDS             198..3920
                     /locus_tag="PKM94_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65048.1"
                     /translation="MSRIGMSAVLGLLLAGGLAASSADRPSALSRRMSAGCPTRDTRY
                     SPRIETYLTPDGRLDADAARRSSYSGPLDVEGFVPTIDPATGAPVFRAALPLAPADYP
                     DDIYWDNRISPSVPGVSGTVRAVAVYNGQLIVGGSFEVAGGVLAHNIASWDGTSWSAL
                     GSGANDWVRSLTVYDGSLIAGGDFDTAGGIQANRIAAWNGLSWSALGAGMDRSVKALA
                     AYDGRLVAGGNFDTAGAVAARRIAAWNGSSWSPLGSGMNGNVEAVTVWDHRLIAGGWF
                     TTAGGISAARVAAWDGSSWAPLASGIADGGVYALVEYNGNLIAGGYFTTAGGTGANHI
                     ASWDGRTWSPLGTGMNGSVYSVGVYDGHLVAGGWYATAGGTAAENIAAWNGSAWSALG
                     SGAGSGVYAVTPYGSALAAGGAFGVAGDIRAVGIAAWNGAVWAALGAGTDDLVYALTL
                     YNGRVIAGGRFTSIGGVQANRIAAWDGSSWSPLGVGLNDDVYALTVYDGRLTAGGRFT
                     ASGEQGVSRVAAWDGSAWSPVGPGVNGDGVYALTEYNGDLIAGGWFDTAGWVVADNIA
                     AWDGSSWRPLGSGMADGDVYALVEYNGDLIAGGWFYSAGDTTAPYVAAWNGSTWSPLV
                     SPDEEFNPVNALTVYDGRLIAALDVGAYKSDAQTAYNGIAAWDGTAWSWLGTGIADDN
                     ASVDAVAVYLGRLIAGGRFITADDPAAYFIASWDGRAWSAMGSGLSGSAASFTVANDR
                     LFVGGRFTIAGGKVAAFLAEWTKGVDTDSDGIDDDLDNCPTTYNPDQADRDVDGLGDA
                     CDTGPELALSPETVDIGWELTRGEFVVKNAGYGTLEFRLDTLPEWCTARPVSGSLGAG
                     GEAVIEVEVDRRGTLMGDHAGVVAISSNGGAGGVLVRMYTPIAPLVGSDSVGIEVGEE
                     IRIPFNGLAEAGSLLSRLQVVSDAGELAYGVAVDTEYVGLRIPVTVVYIYPDEGWSFP
                     ELAGIRVMFADSIVDAHGVPGPQGYAFTYVNGAVVWPGDTDGDGVVDERDILPIGLYY
                     GSGGPGRGEGQGTEWGPYSVAVLAEGVVWRPLRAVYADADGSGEVDALDVCAITENWR
                     ARADGWKRGVGEGAGVEGLPKELSPGVAAALRAALAECPEGAGKAALAEVLGGIGEPV
                     LPGEVTLYQNYPNPFNPSTVVEFYLPAAGEIRVGVWNVLGQEVAELYRGWHASGYGRL
                     EWSGRTRGGGEAAAGLYFYRLTAGDRTLTRRMLLLK"
     gene            complement(4003..4908)
                     /locus_tag="PKM94_00010"
     CDS             complement(4003..4908)
                     /locus_tag="PKM94_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65049.1"
                     /translation="MNRIWTSTGAALLAGAVLAAFAGGCADTVTGRQPGRTVTVAVQL
                     QKNTSALQESFQAFILTVARVRDGVLLAADTSYDLTASVVTVTVENLPSGEPLEFVVS
                     ILNDAGVEVYGGRQVQEIAPDLTTVIDLPLTPSVPMLKFVPRYIAPLDSGAVLDTVIQ
                     ACFYRQPALYGIAFRVYYRNDLVRIDSVGPSPALAALVAGGEVLFLPPQFVQVQTGLG
                     YWAVALTQTVPGRALVDAAARGPLVDVHVQSLGVGAPAAASTSIWLAPTSMTLTDGTA
                     IDSTQVTAVLYTDDCYIDAGSPGPM"
     gene            complement(4932..6053)
                     /locus_tag="PKM94_00015"
     CDS             complement(4932..6053)
                     /locus_tag="PKM94_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65050.1"
                     /translation="MGNSHALRRWAALLLAVAGLASAAECQVIYGQPASGRASFVYTS
                     WDLDSTGAGTTSIAQSLLIVSGFLPLRDNLEARFFLPTAAGDLESPEAAADVSGLGDL
                     RLQVSRSLSNDQILLSIGLSLPTGKKELRFADERMIVQVLSENFLDFPVRRFGEGLGL
                     NALAGFARNYGRATVAGGVSYSYTGAYSPYEGIDDYNPGDVLSANLGADLKAGRAVWS
                     AAATLSYRTTDTREGAKMFRQSTDLDFRLANQTQLDRLSLLLLTRYLLRGESALYDQY
                     EESLNRRLYGNEFHLSGRARYAVSEGWGLGPAAGLRLIAADDEFGASTVVRLGVEASR
                     KIAAHGSLDVDLRYLTGSADGGAIDLSGWQASVSLQAAM"
     gene            complement(6043..7353)
                     /locus_tag="PKM94_00020"
     CDS             complement(6043..7353)
                     /locus_tag="PKM94_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD65051.1"
                     /translation="MRMRMGLALLGALLMVSCAPNLVTQGRKLTDQARYEQARECLFK
                     ELSVNPTNAAAWREVGIAYYKEGRAAEADVAFKNAVNQPAPDPAAYVYLGLIQENKGY
                     PDSAVGYYIAALDRYPTGRSAATVRKRLDQLLQEELRNRLPQVVAGEGQLDASAIPEN
                     SVAVLNFGGARLDPEVAPVARGLSALVAADLAKVSSIRSIERLKVETLLAEQRLRDMG
                     VADSLSAVQKGYLLGAHTVVTGELMSIGDKIRLDAALVDVVRATPTPSAPTEDALDQF
                     YRFQKRFVFSLLDSLHVTLTPEERARLDDVPTESSLAFLAYCRGLQYQGEGYYKAAQV
                     EFQQALKHDGSFSQARTELDNTNTLMSLQNMGDVSAGQVESAVRQDFLGGAPDDLGAS
                     LQGLHDDLGIVPADGEQDGTSDSEEPPVPAGVGAAQVRGNLDGK"
     gene            complement(7406..8449)
                     /locus_tag="PKM94_00025"
     CDS             complement(7406..8449)
                     /locus_tag="PKM94_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOD65052.1"
                     /translation="MSRFRMRLVAALLLSAAALPAVSLVAQTPADVPGELEVVRQHYA
                     RGDWQQCLDRLDKLLARPALTAYDSIAVFEMQSLVYRMMGEDYLERSYTCLQKIAAIS
                     PCLVRLPRDVWPQDLKNRWYSVVSPLGALTCKSTGPTEPQTVAVWYFDNFSVQKYLDE
                     LGSLGSALGLMFQEEFKKLGSLQVVEREKFDYIINEQKLAAGGAVDQATAIQAGKILQ
                     AHVMVFGSFTQLDRSHTMVQARAVNVETSEIIASVSAEGNPDKYFQIVKDLVQELCQA
                     LDVKLSKDDMKLIEEGGTGSYDATVAFAKGVEYEKKHDYKLAYEHFKKAFELDPGFKE
                     AETKMNVYVHLAA"
     gene            complement(8544..10283)
                     /locus_tag="PKM94_00030"
     CDS             complement(8544..10283)
                     /locus_tag="PKM94_00030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD65053.1"
                     /translation="MNEQLIGDYRVIEERGAGGMARVYLAVHREVPSLKVILKVLKDP
                     QHAERFRQEADKLALLEEDSRVCRIKGFFQWEGDLIIVMEFIDGVTVKDLIKSSGHLP
                     VREAVLIAVETLDVLAFAHRKGIYHRDIKPSNIMRDKNGAVRIIDLGIAKAKTDPSLT
                     AVGGCCGTPEYMAPEQFSASDDTRFDLTDIYAVGMTLYKCLTGTLPFAGGDLFAIRDA
                     KLFQDPKSPHAFNREVSPALTAVVLKAIHRDPAKRYQTALEMKEALVSASGVTLPFRY
                     ATPTPPPPPPRKPARPGVKWAAAGVLVAAAVAAYLFFGRGQPSGPDTTAALEPPPVPV
                     PAAPPDGGRLEAGAAVRLVWSSAGDSTVHYRVQIADNADFLNSATYENLSDTFRILDT
                     SFAAEVMYWRVRAVSREGLASAYSAPGRFAVAAGPAATSGTLKVTANRPVRLKLDNAV
                     VAQRVTSWDTVLAAGDYRLEANHPGAAPSTQQQPVRVVGGRTARVSFEFALPADSGVL
                     FVGSDPVGNEIYLNGRPRGSTPQFFKLPVGEYRVQVKKGDVASSPEVGRIVQHGDTAW
                     FMYDFTGDSVAVE"
     gene            10632..10901
                     /locus_tag="PKM94_00035"
                     /pseudo
     CDS             10632..10901
                     /locus_tag="PKM94_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834410.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            11740..12216
                     /locus_tag="PKM94_00040"
     CDS             11740..12216
                     /locus_tag="PKM94_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HOD65054.1"
                     /translation="MVADPLCEGWPVIDEMGKTNKRFSMRLAEGWEDQTVHLFVGPAE
                     RGMNHHLTIAVDDIPLAETAQDYAEYRVEQFLDTLPAAEVLKEEPLALGEDLDAYEVV
                     IKFVPSEAGPRFRRLVFVLHENTAFTFAADFTKVTRNTLGAQMIEMIKTFRPEAGA"
     gene            12231..14444
                     /locus_tag="PKM94_00045"
     CDS             12231..14444
                     /locus_tag="PKM94_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65055.1"
                     /translation="MTALKSTKATDAEHEVKLEPRLISLSWRSGKARAGGIARFEVVT
                     EFVGNGAKVKVTGKSEKGKKLGAVDALMRRNRLTGQFDVPADIDLDDRVFFEVRLPGN
                     DLEGTSDLIPAAPPVKVNSLRWSCDEARRDDEVALSAFVDGVDEAEEVALTIYEFDRD
                     GAHDKITELPGQVVDGRVEKKWRFEYHEDVDEIPTQEEMERYGGAYNPPEYFFTITVD
                     GVEFGKKRASPHLLTFRDWIELSLCDSSGRPLANEDYELLLADGTKRTGTLDGEGKAK
                     VDGVPPGRFQLKLANVRGSLAHGRPAVQQTCPKARAIAKVEGPSRIRVGGRAEYTAKR
                     AQNADQSAPVKWRVSDIESEEVLEVETEIQQDTLILPCIPDSFKGKTVKVEAWQEEPE
                     DGATFSSLVRYDEVGEYIARCRKLENAFPDWTGEAVLNALRRLAGCDDKRFQRMFGVA
                     AADNLQEMCDSRRLPSGVAADDIKWLIEHSRHREKIDPPDDRSGAMKLKEQPQQKLEA
                     DLRGCATDCLGYPLAMGHVLCGISAGQHRNTSVRPHFLVVPGALGWHVDNLYAVTVAG
                     DLGQSAVCVFLGDKSVLIGDDRATTEATYDELRGDVDGFVLGHHLKSLLNGRSLDKLP
                     SDGGLRLSELLQTYYCVCEATASSSLPTAATRFAAFKRAGRHHLLDEIRQFALTFGYS
                     KPIFQKLRALLVQKNFDESGEKQPEPVKHMKALIQRIYDEFLAWLDKHADAEAKR"
     gene            14519..16027
                     /locus_tag="PKM94_00050"
     CDS             14519..16027
                     /locus_tag="PKM94_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD65056.1"
                     /translation="MTPDHSDGGAGGGAGGSGAASGWTPPIVRPAEREDTPPPAAAPD
                     DDAPAAESDMSAAAGSPPAPTAGKSDSPTVSATGLVTGNKYAICVRDSYLNHLEAEFD
                     LKAGDIDDEGVRRLKRRGKKRKQTTPDGWVVELQDHLKKFVIGDVGESDGIFGRKGVL
                     KAVQAFQAAALTKHRWQGKELKEVQITYKGKIDGLVDRDVKEEITRWLGNGYTAYYPK
                     LTASVGRRKNKKGKVTEGANRKPDVLLVQDRLRELGYVAALPSSMKITDGVCDDGTVM
                     AIKLFQAQAYDIDEHVKTKRGRCVPLPGGLIQKGKEDEKRLLGSNPPRYKEVAKGSIK
                     PIQAGIKKRKDEATGDEKKIWDKLEEVWNRVSPHFPEGTNMSSGYRSDARQREMLRES
                     YNETYKADLIKKFGKDEWEKYKKMQGVDDVKAEPEMRSMLAKVGFQIALPGTSSHRSG
                     KAIDVSDNAGNVYDAQQIRALLWAHVMLDEKLVAKILPETAKNGKVVHWEFQ"
     gene            16024..16668
                     /locus_tag="PKM94_00055"
     CDS             16024..16668
                     /locus_tag="PKM94_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65057.1"
                     /translation="MIVVPCLRRTAAAAVACLVLALACGCAKDGGKTDGTAAARPKAG
                     EGILRPDATTYFPLEAGSYWVYERYTEGEEEGDTTRYEVLALDESDSGWVAPVLVTYS
                     DGDTDTITYVCGRGGLVTRKTTFEDVAEPFALLQPVDSATVGDFTYEQCPYGADSCWF
                     LENNPERDVDWVGYEFRQGIGLVSTSVLYMSIDLVDYRIGSAGRDSLKAGNSSP"
     gene            16847..18298
                     /locus_tag="PKM94_00060"
     CDS             16847..18298
                     /locus_tag="PKM94_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65058.1"
                     /translation="MDIAKYSSDSRAVIKVAREVAAEFRHPEISIEHLLVAVVRHEGS
                     EVESILNQLKKNPATVAAMVESYLKQQPSRAAARDSLSASPAVQAVLAQAFEEKGKLY
                     DALVEPEHILLAIFDRASALPPYLREQLEFTKEDLYQAMAESRSVEAIAVSPPRGEGS
                     AAPAEEPRTVAAVSRYCADLTGRAAAGEYDPMIGRAEELRQVIQILLRRRKNSPVLVG
                     GAGVGKSAIVEGLAQAVVSGAVPKALQGVKVLEVDMGGLVAGAKYKGEFEERFKALIG
                     EVIKSAGRLILFIDEVHTIMGAGSGGAGMDAANLLKPALARGQLRLVGATTEEEYTKH
                     LEKDKALLRRFEKVKVGEPPFDEAVAIVNGVAAKYEEHHRVTFSPEARVAAVKYAKRY
                     LSEKNLPDIALDIIDEAASEFGVKKEMAERLLPGLEAAMKDIEPLLAAADGAGLSDED
                     QQKLRALYETFAGQLDALGGYWGHRLEVPEVSG"
     gene            18298..19401
                     /locus_tag="PKM94_00065"
     CDS             18298..19401
                     /locus_tag="PKM94_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65059.1"
                     /translation="MARPELTLKDIREAFARQQQRCRDLAEVVRNLQPVIHESDIGAV
                     VARRTGIPLSKMMTAEKDRLINMEAHLASKIVGQEKAITSVANAIRKARAGLKLANRP
                     VGSFLFLGPTGTGKTYLPKLLAEFLFDDKNAMVRLDMSEFMEKHSVAKMIGAPPGYVG
                     YEEGGVLTEAVRRKPFSIVLFDEVEKAHPDVFNILLQLLDDGRLTDGQNRTVDFSNCI
                     VVLTSNYAADLILDADREGREVDMDQVREFLFTKFRPEFLNRLNDIIIYHTFTPAQVE
                     TIAGLEFAQLARMLAEQNIAVSLSPEARAKLARDGYTFELGARPIQRVIERDITNRLS
                     VEIITGTLAPGDAVVIAVQDDQYVFTRTGESRG"
     gene            19429..19872
                     /locus_tag="PKM94_00070"
     CDS             19429..19872
                     /locus_tag="PKM94_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOD65060.1"
                     /translation="MANNFWLGARERKVKDDSIPVELLPSNKTLYVAKLNNDEAAGVE
                     PVECNNLKQVFEKFQPSFSAEVETLDGQSAPAEFKIRAMKDFQSKELIEQNEYLQKVY
                     FSKEILSDLDKQLKKNTALKKTLEDPQTREALLKAAKYYVELLSE"
     gene            19890..21311
                     /locus_tag="PKM94_00075"
     CDS             19890..21311
                     /locus_tag="PKM94_00075"
                     /inference="COORDINATES: protein motif:HMM:NF036663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65061.1"
                     /translation="MGEEEKRAASSGPQADAKPAKAKELLSDEEFAGILGDAFGDFKK
                     FAGLLPSFKTADGDLVRGVEWLDPKKEFQRKEFLTKPDHARQRRLLAERLKLWIEMLA
                     EGGSIDDLRKKLNEKAAQLESNLEKNMKKVHKAARDLETGYRAIDKFFANAQAEPDAK
                     VNAWFANVSAEELMDPDDKEKFEQLSKAIAAEFREWGIKRCYSMCVVPGYLGSVQNID
                     TLARQIGLPNRVHVVTDLPDLESVEDIMEMLEDPSMDGLMSSDECKQYVSLFANYLLA
                     RSANQYEDGDMWVPPSAAVAGKMYLGDETVGMQQPAAGYKYGKINEAKYLRFRANQPD
                     ASKVNEKGVNPLVSFEGQAVAMGSSTLSNKETFNVYSIRRTYDYVYKTLRNYLNKQTF
                     NVIDQKFIDTLRKDIDKFMRAISGGDNILQDYTVQVYADEEMRKRQEVDIKVALNPKY
                     PARTFNIEFTAWNEDNQTAVKDK"
     gene            21396..21806
                     /gene="tssD"
                     /locus_tag="PKM94_00080"
     CDS             21396..21806
                     /gene="tssD"
                     /locus_tag="PKM94_00080"
                     /inference="COORDINATES: protein motif:HMM:NF037603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HOD65062.1"
                     /translation="MAHRPKLEIDGVEYPHVYSVSYHVYTAKDETGRPTDRAHAGLIK
                     IKVESAEDGNVDIARWAMDTSKPNWKSGTVVFFNKDGAVMKELSWTDGFITDFRETIP
                     DVKNNPDEQIYQEFEISSNAIMIGDAELQNYWDK"
     gene            21867..23762
                     /locus_tag="PKM94_00085"
     CDS             21867..23762
                     /locus_tag="PKM94_00085"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOD65063.1"
                     /translation="MTPEFDAEFRARRADVGADALARRAENVPLDYGTPVAALLRVAG
                     KEIKADIHSVRLNQHVFGHHVMQVLVRRVGLAEGGSGEFEQISESAGFLGKPVTLELR
                     PEGGVVAGDRRLEFIGLITEVRFDHTIDGINSVLITAHSPTISMDLVPRTRVSPPGGA
                     CGTIIGGILGEHPITQGRVQSSPVASAPAGLNLEVDSGVQYFETDYRYLRRLASEHQM
                     FAYYDGRAFHVEKAAANNEQVLKWREELGSFAVGIGTGPLAFRGQVWDPRRKEMVTGS
                     ADRSSIRAALSPLSRTALDGSERVFTTAGVSLRVKHPDQSKVDGALAREVEASAGQMM
                     LCRGTSIVPAVTVGSCVRVRGMDELDGQYWVTAVRHEVSDSGKYYNEFECTPLELALP
                     PLHTERHRRGYFQTGVVTDNNDPDGLGRVRVRLSWQAEDQASEFLRLLTFDGGQGRGW
                     FGLPEVGDEVLVAYERGNPDAPVVLGCLYNGQDTPPKATADCLDGGQVATKIFRTRRG
                     NELVFDDRDGTETITINQRDNTNKIVLAMDGPKIVIETTGDLLMKAAAIAVESTSGDI
                     SIKSAAALKGEAAADIDLSAQANFKSEGAMNYEAKGGVEFKASATQATVEGSAMTTVK
                     GGIVKIN"
     gene            23770..24144
                     /locus_tag="PKM94_00090"
     CDS             23770..24144
                     /locus_tag="PKM94_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOD65064.1"
                     /translation="MELLSLPLLPDAGHFPRTALDRSIVYSIGLILTSRLGMMPFLPD
                     YGCDLWEREFTDLPTANKANIRASLRNSIDTHERRLYNVSVSFSEAQTSSGHALGLLV
                     RVTGNYRDGAEEKKLQADFHVG"
     gene            24171..25832
                     /locus_tag="PKM94_00095"
     CDS             24171..25832
                     /locus_tag="PKM94_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOD65065.1"
                     /translation="MTDHRGRSQAQIFAELYRHLRHFDPTMPESAERLDPILRILLQA
                     NAFQLAQIHQRLDQTWNEATASLIKSICPESGHWPVPAHTIMRCTPVDDAVEVDTYTR
                     FYYKEEREGGQTFYFSPLTATRVLAAAVAHIYLATDRTAIEAVPAGSAAGAPRRTRTP
                     ADAADLRAIYIALSHQGLRSALDGAVAFLSGPPEIQGQLMWGEWTPGRLDGSFSGDAR
                     MCPGVDARWPGAADSESGDWGGLRRSEDIFVKHRNAFVKIPAAFASAWEPGPIDRRLR
                     ALCAAGGVAVPGDHERFYWIRIDLPPRGSRAAFAEPFDLCFDAVLAVNTHEQALFKHT
                     GGNRLVEIEIPDDLGSVLEVSAVTDSAGKTYRASYEIGVSLDRNVYSLEARGDRLVLW
                     FDFSESLDPPPDSITVAYTVTAGTAANGIGKGKITNLYESHPGLSRVANITPVSGAIP
                     SKTEAQILAEVSARLRQRDRAMTFRDLETWALTFDPRIKRAEGRNSVERLNGGICRCV
                     VVRIVIDAEDFHSEAEVDLLRSRLVSFLKARAAVNTIVIVEVVPQ"
     gene            25853..26812
                     /locus_tag="PKM94_00100"
     CDS             25853..26812
                     /locus_tag="PKM94_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HOD65066.1"
                     /translation="MPDLCDRRQKLHYVTAIIILHKLGVDPGAVHLRAVGPFLNYRGE
                     VHSQEPAPGTPLHPDTEISLDVGYSSVVDYLPYQFFHGLEGVSRLSGVHWEDRARALM
                     APFDGSVIRWTAEARHEVIRFSGGATDRRHVLRVLDLFGFSLETAAMTTSEALFWLTV
                     MPTFNNWSGNPARVARILQAVFGLPFSIAENVPLPQEIPQDIQTRLGQQFSRLGNELV
                     LGREYTDRDNGCEVVVHEVPADRAADWIEGGACRQRLANILEFSMPGHMDCRIRIEVR
                     EEPAPLGEGPAGAYLGASLRLHREPGAAAVRPVNEVDSTGSRL"
     gene            26842..28284
                     /locus_tag="PKM94_00105"
     CDS             26842..28284
                     /locus_tag="PKM94_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65067.1"
                     /translation="MSNFRLYSVNWQDGMLVARKHLLDEERYVEDLARWYAYPVGERY
                     GLARKSSDGRPALGLTVLVHGARVRVELSRCQAITSDGTVIEINESGPEVLFAEAPLQ
                     GDRIDVFLAVNREAKRPVGAPDARELVPRAPFAARDYALHLGEPPALPESDFLQIARL
                     KIAGGDVVQAPDYYPACLSMYATDGAYEKHKEYCNRLESLMAVISRAHLVAATAPPAG
                     GRNELPAGIEALCDRVGAQLAATFDLLALGPAAPHPHEVVVFFKRLFRTVATALAYRP
                     AVKDYLNDRLFSRTLGSSMGAFVSQIDSFLLTEYNHRGLSEHFHAIDAVLRLLQETFS
                     FLAQSDRIAPEPSITETVEYQGRTYAMAATSGLRCEERDGLCYLLVSLPAAISLSDLV
                     VLMAKELFDVSQWNAMQVRLGMNDALGLGQTDPVKVDTVTYGSKVVLRAQDMVRNAAV
                     KQVNLIFRGKGDVRKFRELKPSELLIYTAD"
     gene            28306..29232
                     /locus_tag="PKM94_00110"
     CDS             28306..29232
                     /locus_tag="PKM94_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65068.1"
                     /translation="MEIKQYIDDLFRYLATYETSYTSFQTEAFLQTYNGIRAVFTALR
                     QQRNQAVETDYTFLDFIQSSPPAGSDLRTVTVQLLITFFESEADVDGRSNQAYTYVRG
                     LRAIKQDAPYLEDHLLPILFRPTALRTNFALNRFLLEEMARFLNSYGPPLDIGRGVES
                     FSALRDDRKILELCRRRLRLGANLVEDRASLEFSLRNAGAFDQLKKQGPLYEQYLKEW
                     GLLRERERFWSRVGRSLAATGRKAKGLFQSSRYSSLMFTQRRPAVALQIGLVLVWIAC
                     AILAPVLWGKYSDSRYRDLDRRVNELRQQVTP"
     gene            29272..31494
                     /locus_tag="PKM94_00115"
     CDS             29272..31494
                     /locus_tag="PKM94_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65069.1"
                     /translation="MDFAKVLSLGKKAGKGSKKKGGAGRTDTGFSWPAGVRIGVYGHW
                     NSGKTVYFTVLNEDCKFARDLQIAVTDNATAAAFLAHREAIWGVAAAAGEGTRIDTRR
                     DRKFPDSTKTDRVLQFTAILGGGDRIPIVSYDYPGDAVAIGTSSEFAPKVRNFTAGCD
                     GLLFFFDPKALPSESDWQSHAASFVNMLRELGPLDRNLSIPIGLVVTKADSLPGFRGE
                     AQVSLVPHGQERTICREYEEFLVEALAFPEVAGNQEWAGSVREILVKTKEFIRVVLGR
                     TLDFQIFFVSSTGSQPEKIGAEKGRSLYRPPEKMSPVGLREPIRWLLQAVVRNRRLAR
                     LRTVKRYVRNAALLWILVCSLAFGWHLWWQLGRTTGLEDSIRHGRTAAATSTEERNKI
                     GTEYNGYAGRWLVAKVFSKFQAPAREMAKLYRSLKTESETSEFDEVIQRLAGVIKNET
                     AWPAYDVVASRLQPSPAYVELREEVARYAADSAAPVGRRAARVMNAWSKFDSCQVDRS
                     DSQRWSNLQATVNSYQANYKPEMNPAEIQLYSAFTAAAAAHAEKVEVQAQVEQAGDQF
                     ADIIKRINGDPTPAFRLDAGAQELRDYLVQLQMDPQKYGKEIKAVTDYLSEVKKWESR
                     QTFNYRVDIVPDGTHLHMQVVGDGEEPNWSDANNQLFADEAGNVMWKVGDVVYVVLHK
                     AHVGQDESWGRSFDSRVILRDPYALFELEKEIKFGQFGSAKISFVPKSLSERLPVLKK
                     "
     gene            31541..32062
                     /locus_tag="PKM94_00120"
     CDS             31541..32062
                     /locus_tag="PKM94_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65070.1"
                     /translation="MCQRTCRQWIGAAAAAFAVVLLAVPSYAGKVELPKGSKLVVAFE
                     GNVSSSVFAVGDSLPIVLQEDVTVGGLVLVEKGARGTAVVAKAEKSGRAGKPGMIAVI
                     FVDLKPKGQYPLLDVPAIALEGGTNELKGKGKKVLSYLFIFGLFIKGGEGKVPAGSTF
                     EATVAESVKFEVQ"
     gene            32186..35683
                     /locus_tag="PKM94_00125"
     CDS             32186..35683
                     /locus_tag="PKM94_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65071.1"
                     /translation="MTLIEEERIFRDLLRGCDGFTGSIFRFILRLIHVHVLEVEDVLF
                     HLNSAVMMPARPAGLTSADGTEDDVSDSDDARVQQQQDAVSGLDVIGVALRQFEFDPA
                     KRLLIAGHTDTSGEPAMNFALSDLRARNVFALLTGNRAEWARISADRHRIEDYQQILT
                     WISGEPRWGWPCDPQGIDNTWGTHTRDATEQFVQRYNAAFASSPADLPADGSPPPPDY
                     RAPLPANMHRQIDGAAGHRWPEPGWAAFFDLYQDHLAHRLGLSVPDLVSLQTNRPRFI
                     SEDVKFVGCGESFPVDDAERSNYRSQLNRRVELLFFDAHEVPNLDAPTMACPADRTRV
                     HERAECPLYNGCHYLPVYISPEDLTAVAYHLQFMYWDRISGSRECVPEGLEIQAFEDG
                     DAPVAARVQYSGGTYTVKVPHNPARTKLYFQFAAPGKWIFTADASSASPRLVDLTPEQ
                     YAALPAHERAHYYDLPAVWSSRNYWTRGAGAAYTGGKRFEEVMAADRQLKPYGANTTT
                     FEQPLVFSLDDIVLLDSVGGSQDIKDANHRGAERNLAAKSRVKLLVVDPATGFLKLYQ
                     PGADLNSARIPFARNLITEPAEDVKIVFFRDGFYPVGRRRTEPSPNWPANGFVVGARA
                     AVRNDSDYHCTWALQETRTEFASTGDFDLHYFHALHVDDEHPISFTISYASMSFMADT
                     RAPAQFGPIPTEAEVDNFRNQGCYRAMDVWNRKQLFMEEIAPSARTVIIRHFYFFDER
                     EHFKPTAPAGGWVNCNFDNQPSDNAADNFETLFAQQALRDAERDALGGKAKFLALVCR
                     NENGDWGPAYQWAIRDSANRPYSLLKLNKSCYQDTPTILNGISVTEHGDTWVPNTMAH
                     ELGHGTGQPDEYIRRQFTPHSGANAGANANFIQHYIPYSMPFNNGSLMYWNCAPRLRY
                     LWYPMRKINVESRRVGSPLRALLPDKTFVARLDRPSFVFTFHRKVTGGNPSVPAVKED
                     PIASEKQYELTSSPVRKLSLELYDVGRDESSRDTFHAAQGGRVYHAVLVVRVLMKIEW
                     TGSWTDNRKRDRIVEIEDAFKAWGGHYRLEGGTGDIRDIYIHFLPGFYASSDGDTESD
                     KHYDVELTRGAETAISTGFWDSSDLEVSRVITGAQLVDYFFNRASGQTGEQALHFLAD
                     WADGRLPGTFTLHSWGGPP"
     gene            35760..36986
                     /locus_tag="PKM94_00130"
     CDS             35760..36986
                     /locus_tag="PKM94_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65072.1"
                     /translation="MVKWPTLIGAFLALVVCAQPAHQENTVTDNLILNPREWGDRQST
                     GYLPVSGRLGQETVWQKAADAAGDSVLPHHLCLIGDRIAVWYGRRLDLRSAATGEPLW
                     SQETLENCSFEAQRDGLVAFDEHGAFRLLRYDGTYGERVRAPLYNGAGRVFVADASAD
                     AVFLCAQMHPGPTSTAGEKVVGPEIILAALNRGANELAWSVIRDGFLIAALISPDGRQ
                     ACLATRDRLLLAALPAAAAYDPAAELEVAVIEIPFDSVIACAYSHAGEILAVAEKSDN
                     PEGRRERALFVLSSAGEERRRLPLAGAGSLAQPPASFPDGDVCLSLGREIWRLRGGEK
                     LWVQKIPAEPGQILITLLKDGSLLAAAGKLLLQLSPEGEEISRRIVDGPITCRPVMDP
                     QGRVYVAGPGGVYCLR"
     gene            37057..37992
                     /locus_tag="PKM94_00135"
     CDS             37057..37992
                     /locus_tag="PKM94_00135"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOD65073.1"
                     /translation="MVNSYGNVLQAIGDTSMVQLRRVAPPGAAAIVAKLEWENPTGSM
                     KDRAALAMIARAEADGRLKSGDTVVEYTGGSTGISLALVCLARGYRLRIVSSDAFSRD
                     KLRHMAALGAELTLVPSEGGLTTKKLILDMIEAARELSRQPRTYWTNQLSNADSIEGY
                     RSLGEEIWTQTGGRVDAFVHCVGTAASLRGVAAVLKERRPAIRVAAVEPAESAVLSGG
                     RPGPHKIEGVGIGYTPPLWDPGLVDEIVAVGTAEAKAMARRLAREEGIFAGTSSGANV
                     LAAIAVARRLGPRATVVTLMADTGLKYVSTDVFEG"
     gene            complement(38025..39434)
                     /gene="gltA"
                     /locus_tag="PKM94_00140"
     CDS             complement(38025..39434)
                     /gene="gltA"
                     /locus_tag="PKM94_00140"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOD65074.1"
                     /translation="MTEEPQRKKIDPHRREMPKQAAEVRRRNFHEVALGYPIATAVAE
                     AKRCLQCKKPKCVSNCPVEIDIPGFIQHIADGDFAGGTRVLKEKNCLPAVCGRVCPQE
                     EQCEKTCLLEKKGCAVAIGRLERFLADWEAAQGDPELPSIPAPTGRTIAVVGGGPAGL
                     TVAGDLIKLGHRVTIFEALHKMGGVLMYGIPEFRLPKAIVAREVNYLRMLGVEIITDF
                     VVGKTRPLARLLEEFDAIFIGAGAGLPWFMQIPGENLNGVYSANEYLTRMNLMKGYLF
                     PDSPTPIKGHTRVAVIGGGNVAMDCARTALRLGAETRIIYRRSRQELPARLEEVENAE
                     EEGVILDLLTLPVKYIGDSDGWVREIECIRMELGEPDASGRRSPVPVKDSNFRVAVDA
                     VVCAIGNSPNPLIPTTTAGLEIGKKGNIVADDATGRTSLARVWAGGDVVTGAATVILA
                     MGAGRKAARSMHEFLMAPR"
     gene            complement(39452..40294)
                     /locus_tag="PKM94_00145"
     CDS             complement(39452..40294)
                     /locus_tag="PKM94_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOD65075.1"
                     /translation="MYKVIDNRMVVPNIHMLRVEAPHVSREARPGQFVIVRAEDDGER
                     IPLSISDWDARDGTIVVVYKNVGATTNRLSNVRPGESLLTVVGPLGKETEIDTYGTVL
                     CVGGCYGIGSIYPIARALRERGNRVIVVGEARSSYLTFWDDKLRAAAHRVVWITRDGT
                     RGLRGHVPRLSEVIGQESGPINRAIVNGCTFLMKRVSDMTRPLGIKTIVSLNPIMIDG
                     TGMCGVCRVSVGGATRFACVDGPDFDGHEVDWTELLQRRKSYLTEEIIPQSTSRCEEH
                     AGNA"
     gene            complement(40287..42797)
                     /locus_tag="PKM94_00150"
     CDS             complement(40287..42797)
                     /locus_tag="PKM94_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD65076.1"
                     /translation="MNSPHDPVTVTIDSRSLSAARGATILEIARAAGLYIPTLCAHKD
                     LTPYGGCRMCIVEVDGMRGFPTACTTPAESGMVIRTHTAQLHEIRTEILRLILSEHPC
                     SCLVCDEQDECRQYSATIRKAGVTTGCRYCPNDGQCELQEVAARLGITEIGYPVYYRN
                     LPVHKDDPFFDRDYNLCILCGRCVRMCQEIRTAGTLTFLRRGPQTVIGPAFDRTHLEA
                     GCEFCGACVSVCPTGALAEKARKWKGAAEREVTTTCPFCGVGCQVRLQVKKDEVVGAL
                     PAEDDLVNRGQLCVKGRFCIPEIVSHPRRLRHAYKLRDGVLVDIGWEEAATLAAERLT
                     ACPPEEFGLLVSANCSNEDLYVAQKFARAVMRSNNIDTTFRLAYGASCAPYVEHLRAP
                     ATLEDLRRAEAVIAIGLDTRFGRSVIGAELRKAFRRGARIVTIHHRPHDLAQIAAHWL
                     RPAAGQEGELLRELMAATAPAARGGRGKTTGGADIAAAAGTIRESGRTVILLGPMLLD
                     RTDGPDLLRAVHAWAAALGAAVMPLPSQNNFLGALLMGAVGELLPGGAAWADTAARDR
                     LARRWQSALPAEGAQWNSSLVGRGPRRKVLYLLGEVIPNPRAGAETVIFQNIFHPPDL
                     TGADLVLPAAAFSETDGTVINGEGRVQRLHRALDPPGDAAPDWEIVCRIARAMGAAGF
                     AFGSAREIHEEIATLIEGFDAFDAPARAPHRWPLAECDLPAAASAPRSDGSATNPFLL
                     LTTNLEHTHRGFAMADRVAGLSRLYPLRVVEMNPVDARTMGIADGETVAVTAEGLDRC
                     WPVCVTAAQPEGLLHVVLPPGELDGANPRRVQVRKCHV"
     gene            complement(42781..44685)
                     /locus_tag="PKM94_00155"
     CDS             complement(42781..44685)
                     /locus_tag="PKM94_00155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOD65077.1"
                     /translation="MSDFTERVVEARRVWEELAASRVPVITIGTASCGRAAGALDVLA
                     AVEDTLRELRLKARVMQVGCIGPCYLEPLVDITAPGRPRVSYGNVVPGRVHNLIESCL
                     VKDNWLPKQALGHFGEAPLPELPETPRFFDLPMLRAQVRIVLRNCGIIDPDEVDHYLA
                     REGYAGFLAALGRPPEAVIAEVREAGLRGRGGAGFPTHRKWEVCRSQAGSEKYLICNA
                     DEGDPGAFMNRSLIEGDPHAVLEGMLIAAYAIGATRGYIYIRAEYPLAIVRLRRAIEQ
                     MRALGLLGRNIQGSAFSFDITIKEGAGAFVCGEETALIASIEGRRGMPRSRPPFPAIA
                     GLHGKPTIINNVETLGTLPHILRHGAAWYRQYGTQGNYGTKTFSLVGKVRRPGLIEVP
                     LGMTLRQIIFDIGGGTRKKFKAVQTGGPSGGCLSEEFLDTPVDYESLTAAGSIMGSGG
                     LIVMDEDTCVVDIARYFLDFTQKESCGKCTPCRVGSRHMVEILERITLGQAAPEDLIR
                     LQVLAETVKRGSLCGLGQTAPNPALTTLRYFRPEFLLHVTEKHCPASVCRELIEYRVV
                     PEKCTGCQRCVSVCPTGAITGPRSETHNLDPDKCIKCRACYEICRFDAIAGDAIIIRS
                     KRSVPNELTP"
     gene            complement(44678..45163)
                     /gene="nuoE"
                     /locus_tag="PKM94_00160"
     CDS             complement(44678..45163)
                     /gene="nuoE"
                     /locus_tag="PKM94_00160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOD65078.1"
                     /translation="MNHMASTAGAPGAAADLSGILRECSGERGDLIPLLQKLQHTAGF
                     ISADGVRQIALALRITENEVYGVASFYSQFRFTPPGRHSIKVCLGTACHVRGGQIISD
                     AVSRELGVAAGETTPDRRFDFQRVACLGCCALAPVVQIDDDIHSRMAVIRLKEVLAKY
                     E"
     gene            complement(45160..46428)
                     /locus_tag="PKM94_00165"
     CDS             complement(45160..46428)
                     /locus_tag="PKM94_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD65079.1"
                     /translation="MVQYYHAHQVNPRKCRGHMNCMRRCPTQAIRVRNGTAVIHDELC
                     IDCGTCISVCPSAAIDPVINPLQAFSGFKYKVVVPSSALYAQFDSSIHPWVIHQAFRR
                     LGFDLVVNVGLTCEYLAKATTRYLDKYTGPRPVISSECPCVIRLIQVRYPDLVGNIIR
                     LDVRRELTARGTRRSLMASTGLGSEEIGIIYISPCPAKVVSIEQPAEKEQSWFNGAVA
                     IRDVYALLYPIVMGLQAEFDEGAVPEDFFFRAGWPILGGITQSLNMDNWLAVSGLDHV
                     MKIFDDIENSRLRNVDYVEAVACMMGCVGGIFNVENPYVARANNIRQRTRYEKGRPIP
                     EAEIERRLTDGDFALEHSVPPRPTKYFDTDLETSIKRLREVDRIFQKLHQIDCGVCGA
                     PTCKAFAEDCARGDARLTDCVFLSNRGGAE"
     gene            complement(46461..47216)
                     /locus_tag="PKM94_00170"
     CDS             complement(46461..47216)
                     /locus_tag="PKM94_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65080.1"
                     /translation="MLEGTDRLDLEVDHVYASDLMSDVLAFGRPNSILLTGLATKQAI
                     ISAHMAEFVAVAFIRGKRPIDGSDELARDRGLVLLSTTLDMFDGCLSVGRVLHNGPQA
                     VNSPAAVKPEEMSLTQTFTIQGDDFASAGGVSTEVKSILKKIGLDPKLVRRVAISTYE
                     GEMNVVMHAQSAVATLSITPRCITVVLADRGKGIPDIEKAMQAGWSTATEEMRAMGFG
                     SGMGLPNIKKNADELNVESEVGKGTTLTMRFYI"
     gene            complement(47331..47666)
                     /locus_tag="PKM94_00175"
     CDS             complement(47331..47666)
                     /locus_tag="PKM94_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOD65081.1"
                     /translation="MTTQDLVERIGLKPLSRFDPRPVTGVFISDMLSDVMAGAKSGDL
                     WLTVQTHKNIVPAANLVDAAAVVVTSGKTVPQETIDLANKYNIAILASPLPVYELVGR
                     LYSLGIGTP"
     gene            complement(47707..48258)
                     /locus_tag="PKM94_00180"
     CDS             complement(47707..48258)
                     /locus_tag="PKM94_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65082.1"
                     /translation="MYDCQHLWKMKDIQFGFVVFEKCFHCHGLRTYFTTEDNPIPDEK
                     YREGVCFWTRMEVAQSFQFNLQCALCGRKEDFSDLMGLLHCTGCLEDCPVEVLQRELH
                     AQRIWVLVAFGYLPKALSAPASEKKLAVLSEYFNQSRDRTRSQIRIVSSSLISNLSLC
                     KGDFIHDSGMLSLEPPQGRRQLF"
     gene            complement(48677..52753)
                     /locus_tag="PKM94_00185"
     CDS             complement(48677..52753)
                     /locus_tag="PKM94_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD65083.1"
                     /translation="MIPHWTGATAGKFLLFLALAAAFIGLTGSSQPGPGVAQPPGAIP
                     PNANAGTEGPDTTFRPYVPGEVVVRLFSDSISVDSINTLFGTTVKEYFGQVNIYILAT
                     ADPAADIEALVENLRPLPGVIFAHPNYVVSPLQSVQGSFPFSDADNVGSYPEQEAALR
                     LRLSQAHALATGAGVKVAVVDGGVNFAHPVLAGAAVSGWDYVDGDADAFDEPGGRNSG
                     HGTFVAGVVHLTAPGAQIIAYRVADTSGRSNGDRVAEAILRAIDDGCRVVNLSLVMTN
                     VHEVLRNTLAYARSRDVLVVAAAGNERLEVPTYLASDPNVVAVAAVDTLDRLAEFSRY
                     GNHIDLCAPGEWIYAPYFDMYAWWSGTSFAAPFVAGQAALKVSQAGGALDWRSLHASL
                     IATAENIYPANPGFFGKLGAGLANPLQALQDTVGIDTASVDPGVLALTLPPNTAVQRC
                     FNLYSTNPNSVFNATVLGGNVAFTTITADSVLGANATICFTLSSSGLSEGIYADTIQF
                     DISGVSNSPIFVPLSLYVSDTAVISQDAAVRPTGYSFWLPEGWVQTIDACAYLTSTNA
                     PAAFTVRRIDTSAASFVEILSPSGMTNDSVCILLHPAGLAPGVHLDTLVYRVSGVTYD
                     LYQPVSLTIGDQVDSAAVSPMYFYFTSPPGGPPQEGRAYLRSTNAPSAYYLETAGGSD
                     WLHVPDSAGVTNDSISFTIAPAAGVVPGLYTDTLYCHVAGAVNTPAVYVTLEVQDSTA
                     GATATAHPTYFEFVHAPWAGGPAQAGVFLLASTNAPASYSVEAALGVSWLHLADSIGT
                     TNDSVRFTVDGAGLAPGFYWDSLICRVTGVEIPAAVQVLLTVLDDTGGTGPSAYASPS
                     WFSFASDPQTPPYAGSFVLSSSNAPAAFTAAVMFSSPWLHVIDAAGTTNDSVRFTVDA
                     AGLAPGFYSDSIACVVAGVPDSVLVPVQLSVAPEDTLGGDSAVAIPASFSLTAPQYSE
                     LLQVRCLMLYSSNAPAPFTASVRHGAPWLTLYGASGMTNDSVCFGASAASLGQGIYVD
                     TIDCSVEGVAAATAVPVVFYVVDDSAGPGPSAWAAPPTVSITAPFGLQDLIDCHFVLS
                     STNAPAPYVAYAANGSAWLSVADSAGVTSDTVRLVINPAAPFSAGTYVDSIACLVEGV
                     ALPVTVPVMLTLTVDSGGAPTAAVTPATVHYFGPYGMLPPTTKCALVTSSNAPAPFTA
                     WVADSVGRFTSLPDPTGVTADSLCLYLDPAGLPPGIHVDTVRISVAGVSPALTLIVQL
                     SLDMPKVSEVTNYPNPFNPETNISFSLTGATRVQVTVYNTLGQEVAVLLDKRLEAGRH
                     TVSWGGCDGAGRPAASGVYLYRITTDEGVVSRKMLLLK"
     gene            complement(52823..55534)
                     /locus_tag="PKM94_00190"
     CDS             complement(52823..55534)
                     /locus_tag="PKM94_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD65084.1"
                     /translation="MVNIGSDCERAARRFLQTGRLGGRTPAELAASCDQLVRRETERS
                     ARRAVGLGEQFVRRARAADRTMLPVALRALGWAYVVAGKYQPAAARYLEARRATRDPL
                     ARARIDRILIDVYMYLGRHDEALRRGRRAIAVFRGRKAEADLAKTEVNCANVLHRLDR
                     HPEALPLYRRAGAFFASQGDELAAAFCRYNEANTLVQLFHFAAAEKLYSSAREVFLRH
                     DHRLRAAGCLYGLAWLHMLEGRFHAALQELAECEAHYRAAGQAREHLLCVLDRAETYL
                     SLNLLPDARDTARRAAMLARRLGLRYEQAKAEFFRGKALAALGRMKAGRTAFRTAAAG
                     FRREGNEGFLAAVRVAEAVAGESGRARARALAALRERMPQGRLPLWEALCDLEILSAA
                     PEDGGARRRLAANRAVETMPHVRAQYVTLLGDSEARRGRRRAATGHWRRAAEILTAVR
                     AQLPPLDLRAAFSERHAEPYEKLIACEWEAHPSQAAAWLERFHTAGIWATEDARLDDP
                     ARTQAEQSLASLARYVAAAAQRSDGRRVRGGGAAPATSLRRQLEEQIRTDLARLERRR
                     GSAAERLDRLGAEIAAASRTMRLVQLHAVSGDIIGLIHEEGRPRGCRLEGAAPAVERL
                     LQRWRFFVERAPYRTAGETAADLADERRLLARLAETLWRPLDVGGPQPVLIVADGALA
                     NLPWAALPVGGRPLIDRHPLVMAPSVRHHLAAARQRTQSRETRIFVGAAGLGAAGAEY
                     APLLADERRQTTVHNPCGRADWPVDSEADVWHFTGHAHFRSDNPFYSFLELADGPLFA
                     ADFRLRRNTVNLAVLAACRTGLSSFLPGEESTGLIRALLEMGARNVLASHWAVADAST
                     ARWIERFYGALAEGSGPAAAVQRAAADAREEYPSAYDWAGFSLFGAG"
     gene            complement(55528..55932)
                     /locus_tag="PKM94_00195"
     CDS             complement(55528..55932)
                     /locus_tag="PKM94_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65085.1"
                     /translation="MAPSEDAVERWINVSLLAPPPSLRTKSSGRVVFDSWRERPAAVR
                     DLPEGLVRRLRLAAPDLDLEIVGEQRRREWRFIARVYRGRTAAGDFVLRAGRQRVVPG
                     GDGFLTWSSSRPPRTVVLISPDREIRFENVSW"
     gene            complement(56138..56692)
                     /locus_tag="PKM94_00200"
     CDS             complement(56138..56692)
                     /locus_tag="PKM94_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65086.1"
                     /translation="MTESINILWRRVVDGDRAAWADLVRQFAPLVMTVAVRSGLSAPD
                     AEDCGQQVWLALYRTRKNVRDPARLPAWLIQTTRRRAVRMRQQQSARRQVEADAPEAA
                     SLCLPDDQVVQLEQAAILEGALARLEPRCRDLLRKLFFSPGGTTYAEIARHFGLSPNA
                     LGPLRSRCLARLRKILAELGYPVD"
     gene            56986..58047
                     /locus_tag="PKM94_00205"
     CDS             56986..58047
                     /locus_tag="PKM94_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65087.1"
                     /translation="MTDTPVASIIANFLRTPLSRRAFDAFFDLSCRRTIVFLRNLRAR
                     HVPLPLAGYAGDDPMRDLAIDVLAGILAPAGGLPCLGLFPYLKRQGCPALPPEDITGL
                     ESLFGIFLYCQVKQQISRLWGEHDPQVALLKRRVRDILASSEYVSWTDPSTRAVLVAP
                     AQDAPHPRPDAFVVEPDMLRRTVLAAFNRTTNRSSWCAAIFRLIRDETGAPPVVPLYE
                     LLALMVEVNAAFLAGAECAPAPLPGPEELCTRAAGDRARQRAVDDTAASAIARYVTLG
                     RITPAEGEAFRKALELYLADFLAGGSDGLPAYFREVMPPEAHERYLQDYKYVFETTLN
                     EGYRRMIDLLGQSDIGLLQ"
     gene            58081..58935
                     /locus_tag="PKM94_00210"
     CDS             58081..58935
                     /locus_tag="PKM94_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD65088.1"
                     /translation="MHVNVTDLLLFLDGKLSGDRRDVVQGHLAECEFCREFLEGLQDT
                     QVVGESDREPIPPPAMQLRDRIYNEALRGRVIPMTPMAPAAPPQEYLLAADGRPEERP
                     AVECLREYFSESPEMVLRVMRSAPDRRQWVQLVTDDPRQAAHVLLRLPDIQKELLTDS
                     AGRADLSDVADADIEALKWEVKLPDVSFTLDELAYDPDRTEYGGETVLESSRADRVRL
                     RFEGKTEGKLVTLEFLNLVGIDEKDALRVYVIEGTEAHALEAPQAGRASFRLSGSPKS
                     LSIRLFKA"
     gene            58944..59387
                     /locus_tag="PKM94_00215"
     CDS             58944..59387
                     /locus_tag="PKM94_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65089.1"
                     /translation="MTLSSLQFARAFGALKAALAARPVGDHRLLALAAFWPNLAAHRA
                     NPSYQSYLDEYTALFAESLAPEHLADCPPENLAAIRTLAREIGSPEQRETVDLALARA
                     WAYAGETERAVMVCAQGAGEVAAAKELFSSGADANPPMTARGRPS"
     gene            59384..61348
                     /locus_tag="PKM94_00220"
     CDS             59384..61348
                     /locus_tag="PKM94_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65090.1"
                     /translation="MIERDRLREAIDRLRAPAPATAARLDKIAAEWDSETGHLWFDRV
                     RCLFVETDGGEATRGRMYTLVGAVEPIGARTRRPGDLAADEVTFEGQLRAPDDPFVGV
                     VYDSLKSVRPLLRGLAGSEPFGSLSRAAGPVGIFPRVAKAALPNLGGDDAASLHAHFR
                     LEGNSRTATGDSIGLAAALVAYTQLLRPEIMRHERFVSSEVAFTGSIAANGTLLPINA
                     ATLAVKLERAVFSTVRYVVIPAANEAEARAALAALGARYPRRRLGLIAAERLSDVVEN
                     YNIVRSERMCIGQFVARKAVHWGRSIKTEVALLAILLYVLLAITWTRTFAPWWFDHNP
                     VRYEILGKELTLLNAAGERLWATTCQCESILPEFAFVRIVDVDSDADNEVAVLVSQAS
                     DCPDNAILRVFDQDGDSLWSRSAIIPGQPAETLLASWNYEVAALSFRDTQNGPVFMTV
                     LNASLNGTRSYIRFFDSVGDPLGWYVHSGYTGIHSWFADSAGRQKLVMQGWNNAYDTK
                     CLFLLDPFSAQGCSPPFNLMSADLMFGGEGNQPAYVLFPLEEISRALGIQHYGAMEPQ
                     VVHGRIKVTLLPIPSQPEAQLIYYFDQSLRVEELDVSEQLALSYKGLVSSGALPSKTL
                     SEGIRERYASIRYWTNGGWVTEGELRAAGK"
     gene            61817..63010
                     /locus_tag="PKM94_00225"
     CDS             61817..63010
                     /locus_tag="PKM94_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65091.1"
                     /translation="MDLPLEVTFSVTNGHEPLSGCHPYRAMFGLRTGPDWWDCPCPGL
                     LTFLPNGDIKLGPWGGATVYTGFALDTWHDVRCRLSLPGDSQLHAEFWVNSSYLGEIT
                     LPEESWMSEPAYLDISCQEGTVWFDDICVGPASEPEFLPVALDIKPGSCPNPLNLRPY
                     RDGAPTLAVDELSDAVPSAKPDPGVRPPRGVLPVAILGTAEFDPTMIDWTTVTLQGVS
                     PIRHSFEDVAAPVPADAVQCQCTAAGPDGYTDMTLKFYRDEIIAALGQVYAGETVALT
                     LTGNLLDGTPIEGVDCVVIINGPEPPEPPLAADDQTPVLLGNYPNPFNPATQIGFSLP
                     TASHVTLVVYNIMGQRVAVLADGQYDAGDHSVTWDASEQSSGIYLYRLEATGFSQTRK
                     MMLLK"
     gene            63235..64086
                     /locus_tag="PKM94_00230"
     CDS             63235..64086
                     /locus_tag="PKM94_00230"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOD65092.1"
                     /translation="MVHRLTRVIGSIGLVCVLAGALGRADAPFAVLAPGLEMATFTPD
                     HRGAVRSPMSRDRTGVTILRIDPAQYEFCLLAQSNGDAPTNLSVAEWARRHHLVVALN
                     AGMYNLDQRSHVGYMKVGDHINCRFPNPYQSAAAFGPRSDSLPPFRIYDLDEDSLRGL
                     AASYTYVMQNLRLIKRPAENRWQPQDKRWRGLALGEDTQGRALVILTRSAWSMYDLNE
                     FLLSLPIGIVAAQHLEGGSEAQLFVRTADTTFAIVGSYDTSLDDDDGQWAGWPVPNVI
                     GIAPKTR"
     gene            complement(64286..65719)
                     /gene="lpdA"
                     /locus_tag="PKM94_00235"
     CDS             complement(64286..65719)
                     /gene="lpdA"
                     /locus_tag="PKM94_00235"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOD65093.1"
                     /translation="MNDTTRNKAQVAVIGGGPAGYGAAFLAADLGLDIVLIDPAENPG
                     GVCLYRGCIPSKALLHTARLLAEVQEAKDRGLAFGAPRVNIRKLRSWKDGVVDKLTGG
                     LGQLVKQRKFRHLRGHARFVDPHTLSIREVAGAEYTFAFEHAVIATGSYPATLGPQQP
                     DSPRLLDSTSALELADIPGTLLVIGGGYIGLEIGTIYAALGSRVSLVEMTDGLLPGVD
                     RDLVAILRRRLDRIFDAVMLETTVRELKVQANGVKVAFAGKQAAGKAQLFDRVLVAIG
                     RKPMSAQLGLDAVGVDTDARGFIKVDAQRRTSAPHIFAVGDVAGEPMLAHKGLHEGRV
                     AAEVIAGRKAAFDPAAIPAVVFTDPEVAWAGLTETEAAGKKIEVEITRFPWAASGRAL
                     TLGRTDGATKLVIDPHSQRLLGMGVVGPGAGELIAEGVLAIEMAATVDDLGLSIHPHP
                     TLSETVMEAADLFHGTATDYYRPKKRK"
     gene            complement(65755..67062)
                     /locus_tag="PKM94_00240"
     CDS             complement(65755..67062)
                     /locus_tag="PKM94_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOD65094.1"
                     /translation="MLKDIVIPDIGEKVESGRVVAVLVKPGDIVGVDDGIIEFETEKA
                     VVEIPSPEQGVITEIRVAVGEELKVGSVIARLDTAAGAAPPPVPPAVEREPAPAAPES
                     KTPAPPPPAAPAPRPVSAPAPPPAAPPRPTGPAPASPSVRRLARELGLDISAVSGTGP
                     GGRITESDLKVHVRRAAASGRAAPTGSAPAPAGSPDLPDFSRWGEIERADMSTVRRVT
                     AESTARSWAQIPHVTQSDKADITHLEEFLRKNADRVKNAGGKLTVTAVLLKVMGEGLR
                     RFPRFNASLDPANRQIIYKHYVHIGVAVDTDHGLLVPVLRDVDRKNIVTLAVELADLA
                     ERARGRRVAPDELEGGTFTISNQGGIGGTDFTPLVLWPQVAILGVSRAAIEPRYIERE
                     FRPRTILPLALSYDHRAVDGADAARFLRWVCDVLESPFAMFLE"
     gene            complement(67075..69753)
                     /gene="aceE"
                     /locus_tag="PKM94_00245"
     CDS             complement(67075..69753)
                     /gene="aceE"
                     /locus_tag="PKM94_00245"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOD65095.1"
                     /translation="MTGRDDQTRAPEIEYENREWLESLDYVHRSQGPGRVRQLLRRLQ
                     MRAQENGVHLPYTANTPYVNTIPVERQPVFPGSREIERRIKSIIRWNAMAMVVRANRD
                     HSGIGGHISTYASTATLYEVGFNHFFRARTENHPGDLVYFQGHAAPGVYARAFLEGRL
                     SEAQLANFRQELAPGGGLSSYPHPYLMPEFWQFPTVSMGLSPLSAIYQARFIRYLEDR
                     GLKRRDDQKVWAFLGDGELDEPESLGALTLPPCERLDNLIFVINCNLQRLDGPVRGNG
                     KIIQELEAAFRGAGWNVIKVIWGDDWDPLLAQDDKGLLVQRMEEVPDGQYQMYSVASG
                     DYIRRDFFGKYPELERMVRHYTDEQLQKLRRGGHDPHKVYAAYKQAVEHEGSPTVILA
                     KTIKGYGLGEAGEGRNVTHQQKKLNEQELRHFRSRFGIPLPDSSLAEAPFYRPAPDSE
                     EMRYLKERREALGGYLPKRLITVPPMPAPADDLYAEFLKGSGDREVATTMVAVHLLSK
                     LLADPDLGKYIVPIVPDEARTFGMESLFRKVGIYSHQGQNYDPVDKETLLYYREATDG
                     QILEEGITEAGAMSSFTAAASAYANFGLNTVPFFFFYSMFGFQRIGDLIWAAGDIQAR
                     GFLLGGTAGRTTLAGEGLQHQDGHSHVLALAYPTVLAYDPAYAYELAVIVRDGLRRML
                     EKGERRMYYLTIMNEFYKMPDMPRGVEEGILKGIYKFRASGNRRAKHKVHLFGSGTVL
                     NEAVKAQERLADAYGVAADVWSITSYKNLYWDAIDAQRWNMLNPEREPRLPYLARALE
                     GEQGVFVAASDYLKALPAMLASWIPGPLTLLGTDGFGRSDTRPALRGFFEVDDRHIAF
                     AALSALAREKKLSLTEVKKARKDLDIDPRRANPLFS"
     gene            70064..71575
                     /locus_tag="PKM94_00250"
     CDS             70064..71575
                     /locus_tag="PKM94_00250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD65096.1"
                     /translation="MHPAPNTRPPAGRRLRFGRKVSALVLVLAVLAAAGAAALLAGCF
                     TNPATGKETFSLYSTGQEIQIGRESDQAIRAQLGVAGDSALQRYVAGIGRNLAAVSER
                     RDLPWTFCVVDDPTINAFALPGGYVYVTRGILAHMENEAELASVMGHEIGHITARHSV
                     TQMTKQQLAQLGLAGAVILKPDLEKYGQLISAGLGLLFLKFSRDDEHQADLLGLRYMY
                     RGGYDPREMDDVFAMLDRVSGQQGGSALPNWLSTHPSSADRIQRITRMVDSLPPDFSG
                     RKVSRNEFLQRIDGLVYGDNPREGYFRGNTFIHPDLKFHIDFPDQWQTANQKQSVAGV
                     SPNEDALLQVSLAEAQTPAAAAQQFVSQQGISASAPQGATIGGLSAATATFSAQTGDG
                     VQLTGTAAFVGYNGRVFEILGYGLAGQWSQYRSAVAGAIASFGPVTDQSLLNVKPRRI
                     EIVTIPRAMTIQEFNQAYPSDSKTTVEELTILNLVQSGEKIPAGTMVKRVVAK"
     gene            72026..72790
                     /locus_tag="PKM94_00255"
     CDS             72026..72790
                     /locus_tag="PKM94_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOD65097.1"
                     /translation="MGLGIRTDISAWDLVRRLNASELALYSSMERLTSGLAINRASDS
                     PAILILSERLRAQTASLERQIENLAARQQKYECASSALMELRSHLTGLRSLALGAAGE
                     GGHEPAMQAAYDTAAQNIVEAYNRVISCAEYNGRYLFDGATGSLAKLTTIRIDLSAPA
                     AAESAIAAIDSAVRQVDTVQIDVGAAQRRDLEAQQRMFTAARRDLVAAESELCGTDVA
                     AEYTSFLAESIRLRAGLALLAHSRVTAESVLSLWDD"
     gene            complement(72957..73190)
                     /gene="rpmB"
                     /locus_tag="PKM94_00260"
     CDS             complement(72957..73190)
                     /gene="rpmB"
                     /locus_tag="PKM94_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOD65098.1"
                     /translation="MSKACDICGKRPATGRNVSHAHNKTPRRFYPNLQNVRAVVNGVP
                     KHLSVCTSCLKAGKVMKNVRGRRPRMVPAETTK"
     gene            73473..74732
                     /gene="serS"
                     /locus_tag="PKM94_00265"
     CDS             73473..74732
                     /gene="serS"
                     /locus_tag="PKM94_00265"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOD65099.1"
                     /translation="MLDLKFIRENPEAVKAGIAKKNDRSDIDALLAVDARRRDIISRT
                     EALKAERNRVTADIARKKKAHEPADDLVAEMRRVGDQIVQLDADLRAVEDDLQTRLSW
                     IPNLPHESVPVGGEADMKIVRRWGDLPAPAFPVRPHWEIGEQLGILDIAAATKVSGAG
                     FYALRGLGARLERALIAYMLDTHAAGGFCEHAAPYLVTEETMFGTGQLPKMTEDMYRT
                     EDNMWLIPTAEVSLTNLFRQEILDYRRLPILVVGHSPCFRREAGAAGKDTRGMIRVHQ
                     FHKVEMVKIVRPETSYDELESLVAQAEKILQGLRIPYRVGLLASADLSFAAAKCYDIE
                     LWAAGIERWLEISSCSNFEDFQARRMNCRFRDEDKKVRFPHTLNGSGLALARLVPAIL
                     ENYQNADGSVTVPEVLRPYMGGLARIG"
     gene            74732..75988
                     /locus_tag="PKM94_00270"
     CDS             74732..75988
                     /locus_tag="PKM94_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD65100.1"
                     /translation="MPKPLSRFSLTNRLYVGKSTFYNGFLLVGVVAISLVFIWYTLNV
                     IDRLKEGTRSQVEKYVKMWQLAANSNTSGEELQFIFDEIIVKADFPIIVLDQNREPIH
                     WRNIKGIGPTENSPKALATLRRLAESMAARNQEFPLHFGEGHVNYFLYGDTDAITRLR
                     YMPFVEISIVLAFLAVALVGYHNIRRSEERHIWVGMAKETAHQLGTPISSLMGWIEVM
                     RAECDPAADRAQLQAQFADGLENMRIDIDRLQKVANRFGQIGSIPELHPADVNRAVAD
                     AVEYFRRRLPFEGQGITLDVRRGELPPVDLNPELFGWAIENLIKNSLQAVDPKAGRVL
                     FTTSYLPHRQCVVIDVEDNGAGIPAAAQRRIFRPGFTTKKRGWGLGLTLVRRIVEEYH
                     RGRVTLKRSKPGETVFEITLPASTQR"
     gene            75997..77559
                     /locus_tag="PKM94_00275"
     CDS             75997..77559
                     /locus_tag="PKM94_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65101.1"
                     /translation="MPGSAEQKRILWVDDEIDSLKSHILFLEKRGYAVEGAVSGDDAV
                     ELVRRKSFDLVLLDEMMPGKDGLTTLEEIKEIRPHLPVVMVTKSEEETLMDQAIGQKI
                     DDYLTKPVNPTQILSVIKRMLEAKRIISSSTMQRYVTEMNRFNQKLFGRLEPEDWYEA
                     GRVLAAWNLELDRHADVGLEQALEGSRKEWNTEFTKYLDRNYLDWLHSDRAPTLSPRL
                     AERFLFPHLREKRKLVFIVVDCMRLDQWLMVEPLIAEYYDVRRDYYFSILPSATPFAR
                     NALFAGLFPDEIAQARPDLYRTQAEGSLNRLEDELFEDNLQRHGFDQARQMKYVKIYN
                     NTEGDQVVRRLADFYDAPVATFVFNFLDILAHGRSNNVILKEIAGSEAAFRTLMQTWF
                     IHSPLFAMLKLFAGRRDTTVIVTTDHGSVLCRRGTMAHGKRTTSTNLRYKYGDNLNSD
                     PNDSILVKKPKDWRLPLPTLATTYILAREDFFFVYPNNYNEMIRQFQNSFQHGGVSLE
                     EMVVPVATLNPR"
     gene            77561..78007
                     /gene="tsaE"
                     /locus_tag="PKM94_00280"
     CDS             77561..78007
                     /gene="tsaE"
                     /locus_tag="PKM94_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOD65102.1"
                     /translation="MQTVLNVVSHSETETVGLAEKLVPFLRPGDLIVLTGELGAGKTV
                     FVRGLARGLGVDPALVNSPSFTIVNEYPEGRLPLYHFDLYRIGNPAELLEVGWNDYLA
                     REGITVVEWGERAGDCLPPRYYRAVFRVEGEHNRRIDIGVVGDDRP"
     gene            78004..78681
                     /gene="tsaB"
                     /locus_tag="PKM94_00285"
     CDS             78004..78681
                     /gene="tsaB"
                     /locus_tag="PKM94_00285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOD65103.1"
                     /translation="MSVQNILAIDTSTALLRLALAFGADRLVQVREAVPQSHGQVLLP
                     RLGDLLAAAGLTVRDLQAVAVSVGPGSFTGLRIGLAAAKGLVVALDLPLVPVTAFEIA
                     ARRLADAPRPVYVIVPLNRDEGICCPINGPLSIGPPVIARYSALPEIVGRAAVAAIGL
                     TIREKMPALANPDLSDRLDYDAGDLLVIAREKLDSGRPLPAAAELEPWYLQKSQAEIK
                     FEQRKKK"
     gene            78659..79135
                     /gene="rimI"
                     /locus_tag="PKM94_00290"
     CDS             78659..79135
                     /gene="rimI"
                     /locus_tag="PKM94_00290"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOD65104.1"
                     /translation="MSNAKKNDLVLRDMAARDLDTVVAMEKEIFPDPWPRSAFEEQIG
                     GDGWGALVVEIDGAVVGYACYFTVDVEAHLTNIAVRPEHRRKSVANMLLGAILGRATA
                     AGCQYLLLEVRSSNNVARTFYERKGFRILYRRPNYYRRPIEDALVMVFYLDGRQGG"
     gene            79141..79983
                     /gene="accD"
                     /locus_tag="PKM94_00295"
     CDS             79141..79983
                     /gene="accD"
                     /locus_tag="PKM94_00295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOD65105.1"
                     /translation="MAWFRKDHSGPVPQEKKNIPEGLWIKCDSCGEIIYSRKLEKLLW
                     VCPSCDFHFRITSQKYIDLLLDGGRLEEFDSHLTSRDPLKFKDSKKYPDRIREAQAKA
                     GRLEGVIAGIGEIGGIEVSFAIMNFAFIGGSMGSVVGEMIARAIERALNRNIPLVIVS
                     SSGGARMQEGILSLMQMAKTSGLLERLDKKRIPYISILTNPTTAGVMASYASLGDVVI
                     AEPKALLGFAGPRVIQQTIKQELPEGFQSSEFFLEKGFLDKIVHRKDLRDTVIALLKF
                     MWRK"
     gene            79971..81260
                     /locus_tag="PKM94_00300"
     CDS             79971..81260
                     /locus_tag="PKM94_00300"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOD65106.1"
                     /translation="MEKVMPAGAYRTAERFLLSREFFGMKLGLENITEFLVAIGSPQL
                     SYPTIHVAGTNGKGSTVSMLAAVLRAQGYRTGLYTSPHLVSLRERVRVDGHPIPERSV
                     TAFVARYRRELVRRKLSFFEVLTAMAFEHFARAGVDLAVVETGLGGRLDATNVLCPLL
                     TVTTDISRDHMEILGRTLRKIAFEKAGIVKSGVPHLIGLLPPVAEEVIARCCAELGAP
                     LHRLRPRDFAAFGESNRLDFHENGWRISGLVPGLIGPHQLRNTALALKALAILRDQGL
                     AIDKQAVYQGMARLEWPGRFQIVARRGRPTLVFDVGHNAGGIAAFVETFQTRFPGRKA
                     LILTGFVKRKEHQKMVDHLAKIAAQYVIVPLDTKRSVDTAHLLGTINWRRVPVHRAGS
                     VATGWRRVARTAQPDDIIAVIGSHYLVGEFFEKIGSA"
     gene            81266..82678
                     /locus_tag="PKM94_00305"
     CDS             81266..82678
                     /locus_tag="PKM94_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65107.1"
                     /translation="MDSKERRRPAPETADPERLPQGEALPAGPGPAAARAAAAAVELP
                     AEKLTRLTEANRQLKRKIFDLYTIFEISRNFSSVLNYQTLLDSFVLTCVAQVSASKGA
                     IYLKQDARSDRFSLAKSKGGGAFPPKEATFPSDSKIADYLARLNRPVPTSELLAGFSR
                     EPEAEALRSFDPGLVVPLIYQSRLIGLVLIGEKIGAKEFGLDDIEFLSILGNQISVAI
                     ENARLYESERAATQQLLAAQQQLVHTERLAALGEMSARVAHEVNNPLGIIKNYLLLVQ
                     RTARGNVETGNYLEIISQEIDRIARIVRELLDFHRPEKTALRPMKLGKVIDDVLVLMD
                     RQFEKFGIMVVREFADDLPLVMGSPENMKQVALNIVLNAADAMPNGGRLDIALRRVAD
                     GVLLAFTDTGPGIPPEIIPHIFEPFFTTKQEGKGTGLGLAVCYGIIKRHGGTITYKNS
                     SIGGCFEIILPALEDRENHG"
     gene            82671..84782
                     /locus_tag="PKM94_00310"
     CDS             82671..84782
                     /locus_tag="PKM94_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD65108.1"
                     /translation="MGNTGERIIIIDDEKRMCDSLYALLTGDGYEVETFQRSPEAAEA
                     IRTRKVDLVISDIKMPGMDGIELLRRVKEVDDGIPVILMTGYGSLDTAIQAVGAGAYD
                     YLLKPVEFSYLELAVNRALEKRRADIARLQLLEELRLSNMILKRRMDDLNALYEAGKS
                     IGSTANLHELLRQIVVLASTVTEAQVGSIMLLDEAGEYLTIEAAIGLDEDIVAATRLS
                     IGSSIAGWVAREGEPLIVRDVEADPRFRRINKERYGAASLLCCPLRIKNKVIGVINLA
                     NKAGGETFNDNDLRLLTTFASQAAVAVDDANQFERNRRRLIEFEILNELSGELTEIQS
                     MHDFCQLLVTKLTRVFPIDCALWFNWEPGRKTLVAAAASGGPDLRPERRSEVTLGPLE
                     LDEDILADIPRLSAAVAADIRAHQALPAPETAFLALPVKRRGELAHIFYFAAAGKKPY
                     SADDISIARLVVSQAALLFEREQSLLNMTRLLTMGNMISEISHDLRKPLTSIKGSLQV
                     LAMKHPEIAAQSELLNAAEEEVHRMNELVRELVDFSNPNKYQTEKIDLRRIVARAAEL
                     VGPDLRKKNIAYSARFAEADWDVIVNKNQILEAFLNLLINAIDAIPGPGQIRVEGLLE
                     TPPHRKEPYLAVRVADSGCGIKKENLSRIFERYYTTKDTGTGLGLSVVERIISAHNGT
                     LAVQSEEGRGATFSLYFPYHN"
     gene            84884..86308
                     /locus_tag="PKM94_00315"
     CDS             84884..86308
                     /locus_tag="PKM94_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65109.1"
                     /translation="MAKQKTKILVIDDDPKVAWLLSEGLGARFEFVAARDGSEGLQMV
                     STEQPELILLDIKMPGMSGIEVLEKLNKLESRPEVIMVSGHGDTNYVVESIKLGAAEF
                     INKPFDVQEIEIHINGVLERRNLRREVKELKSELQAQSAYANFLGDSVAMTKVKEIIE
                     QVADSELTVLIRGESGTGKEIAARSLHQLSARKDRPFIKVNCAAIPRDLLEAELFGYE
                     KGAFTGAHKTKQGRFEIAHKGTMFLDEIGDMPMELQSKLLQVLEQQEFVRVGGITSIH
                     VDVRIICATNRNLEQAIHDKEFRDDLFYRLNEITLFLPPLRERPEDIPLLVRHFIDKY
                     NQLYSKDFRELSPSTIDRLMTFSWPGNVRQLENMLKQVAVRGDESIIIDLINSAAAAP
                     TMPSRSTYTPATTDVGPTNAAGTETYSLKERIGRTVAEEEKRLISEVLTRTNWNRRKA
                     ADLLEISYRSLLYKIKDYNLNSSK"
     gene            86599..87018
                     /locus_tag="PKM94_00320"
     CDS             86599..87018
                     /locus_tag="PKM94_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65110.1"
                     /translation="MDDCNFIDKVDLELKRAERYSVFVSLVVFDLSFLENHLGPGASS
                     VVHSLSQEARRNVREIDVVSVMDGCKLVLLLPETPRQGAEIASRRVSEILRDRLSRDN
                     SAAAEKIIPLEMASYPDAAGARTIRDFLGDYLDRHRN"
     gene            complement(87171..90455)
                     /locus_tag="PKM94_00325"
     CDS             complement(87171..90455)
                     /locus_tag="PKM94_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD65111.1"
                     /translation="MVRVSIVIVILLAAVPAAGAATRHVPEDYPTIQAAIDSAAGGDT
                     VLVAKGNYSGPGNLDLATRGKNLVIRSDTGPDSTRIIFRLTVGEQWFTRCFLFDSASG
                     GTVLEGFTFVDSGSYTATEGRGLLIQGSSPLIRRCVVSGFHAGSPVVCEGGSPTFDHC
                     TFTGNTGGCSTCKSGDDRAAPWLGRASAVRCTNGATARFDNCDFVGNTADLSQGALVC
                     DASSPLLTDCRFVDNGGYASHAAAVTAGNGSGPVFAKCLFERNRNDGLNREVFGGAVY
                     LYGSTGSFDGCLFDSNATLGGNRRFGTGVCADSGSAATLAGCTFTRNEGGGNSGFSAV
                     WCGPDATVAISRGLAVFNRDCPPVGRADSTGTVTLSCSDLYGNSGGDWTGDIAPQFGL
                     DGNFSLNPHLCDTANPAVGLFDISPCAPPHSPCGEVIGAFGVGCLDARPMILSPAAVL
                     AMEDQPFGYEPEFADPDGPDTVISFGAAPGWCSVFQNTLVGTPPEGASDTLFVLVVSD
                     GYKADTRTVVIDVIPVNDPPALDTIGDQAVDEGEQLEFRVYAADDDSDKLILAAEGIP
                     EGAVFVDSGNGAGGFSWRPGYRQAGAYEVWIAATDDSLAADSVRVGLTVRDINVAPVL
                     DPVDSQWVIEGRTLAIPVSAGDADGDVPVLAAEGLPAHAVFVDSGNGRGRLSFTPDST
                     QNGVYDVLIVAADTALADSLTVTLIAVDSVPVIAALVFAAETDMQHVVGNAPTVAWHY
                     TDSLQARMQTRFEIAVGLDSDWTTAELWADTVFDSPSTTVAYAGAPLADGLSGRVRLR
                     AANGAAWSEWFEASFRLNSRPGELAALAPSEGSSTGARPMLRVTNAADAEGDSLAYSF
                     VVFLDTVPRTEDSQYVAGLPEGIDSTGWQPPLSLTENRRYRWRAQAFDGYEYSDWTDS
                     TGSLFWVNDAPEPPPMPHTTPITSGCSLLDLLPTFTWSRSLDPDPLDTIVYFLHLATD
                     SGFVALQVFGPLADTAWTPADSLAPGTAYWWKVATEDNTGQRTESAVTSFWTWLPGDA
                     NDNHDVAVSDLTLMISHLFRGGSLPCEFRTGDVTGDCAVTVADLTHMISYLFRGGPAP
                     LLWCAE"
     gene            complement(90534..92285)
                     /locus_tag="PKM94_00330"
     CDS             complement(90534..92285)
                     /locus_tag="PKM94_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD65112.1"
                     /translation="MGRAVIILALLLAGGVSAATVEIDPIPSGRLRLRVYASAVLTPA
                     DSFQTLHSGLVAVPGEGIYAYGTPLTSGGVDTEQLSVWLLDASVDAGSEGILDLDVWH
                     TPAAPGGDTLINPLVPDSIFRVPDNSLFAWADSCRCVHTSDPFMYLDREGTKSFLVRV
                     SRVCYELGGDERWVNFVYAVRWTQAGAIDTAHVINGYYNRGSPLAPVHASLGLWAPSL
                     TVEAAVYRIFTVEDTGAGRTVVLWETPDPITSPAAPAEWVGQRKVIGNPAAEHHALAV
                     VQWTLPHPNQHWHTTLHSPRAGRWEGFATGGLVDDQPVFFGVSSDLEHFTTHPEPLIP
                     RSSERPGTPIIDSTVYTVRPLVLRVGDTSVYRLAVSGARDTTDRHTWCSTALTMRVID
                     LQTPTAPAPYAPADPGCPPLTLYPTFVWGASTDPNPGEAVSYCVYLAPDEGFEAARAS
                     PRVQDTSFVFSEPLARSTRYWWKVSAEDSSGHVRMSPAATFRTWLPGDADGGGTIAVA
                     DVTLAAAYLFRGGVLPCGEAALDVTGDCAVSVADLGRMIAYLFRGGQPLSAGCGGDAG
                     RTRGGEPGGRISSPAAD"
     gene            complement(92549..95236)
                     /locus_tag="PKM94_00335"
     CDS             complement(92549..95236)
                     /locus_tag="PKM94_00335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOD65113.1"
                     /translation="MNKQNETEIKKSAAYAPAEVEDRIYRKWLEAGVFVGDPHSPKKP
                     YSIVIPPPNVTDVLHLGHALNNTIQDILIRRHRMMGYEAEWIPGSDHAGIATQVIVEK
                     RVAKEGTTRREMGREKFVERTWEWANQNKDKILSQLKRMGCSCDWSRTRFTLDPGLSR
                     AVAEVFRRLHEKGWIYRGFRIVNWCPSCQTSLSDDEVEHAEFDSHLWYIKYKLKGSDE
                     YLTVATTRPETMLGDTALAVNPKDTRYKKYVGKTIILPILERDIPVVADSYVDPEFGT
                     GVVKVTPAHDPNDFEIGKRHDLPEINILNADGSLNENAGKFKNLDRFEGRTQLVEELQ
                     RKGHIDRIEKYRLAAGTCYRCHSIVEPYLSEQWFVRMDQLAQPAVEAVKTGKLRFHPE
                     YWSKTYLHWMENIRDWCISRQLWWGHRIPIWYAEDGTVFVSAERPRKEDLPAGCDPEA
                     LVQDEDVLDTWFSSWLWPFSTMGWPERTAELEKFYPTKVLSTASEIIFLWVARMVMAG
                     YEFAGELPFSDVYIHGTVRDANGIKMSKSLGNGIDPVEIIEKYGADALRVSLTLATPD
                     GQDPWISKNTFEVGRNFVNKLHQVSRFVMMRLEGRPPVRDTIDESDLVLFDRWILSRL
                     ERTIRAVDRNFAEYRLSAATKTLYNFVWNDYCSWYIELIKPDQPGQAIRPGSLDVATY
                     VLDKIVKLLHPFAPFVTERIYLDLIGADLEAVDRKTTIVFGPWPTTQGRFIDDQLESS
                     LEQIQAVVNAVRSVRSELNVPPGRKSDLYVRVDNPQFGDLLQKHIEYFRSLARVENLH
                     CGTAVKRPSPSATAVITGAEIFVPLEGLIDLEVERNRVERELQNLALQLEKVSKKLAN
                     QDFLAHAPAEVIEREKAKKADFQDRIEKLNKNLEQIMGW"
     gene            95394..96185
                     /gene="mazG"
                     /locus_tag="PKM94_00340"
     CDS             95394..96185
                     /gene="mazG"
                     /locus_tag="PKM94_00340"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOD65114.1"
                     /translation="MDTIKDRVLDPRRPPFERLVALLAILRSPEGCAWDRKQTHRSLL
                     PYLIEETYEVVEAVENDNPAALREELGDLLVQIVFHAQLAAEAGRFTIDNSIADVVEK
                     LVARHPHVFGERKNLNPRQVRDQWEQIKVESGEKDRVLSGVPKAMPALTMAYRVGEKA
                     GGVGFDWNSPEEVLGKIEEEIGEIRARLAVAEPEGVAEEIGDLLFAAASLARKAEVDP
                     ERALKQALDKFRRRFERLEDAVNGSGRRFRDYSLEELEAIWQSIK"
     gene            96596..97855
                     /locus_tag="PKM94_00345"
     CDS             96596..97855
                     /locus_tag="PKM94_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65115.1"
                     /translation="MKKLVMLSILALCVLSTSAFATETRTLTLGQTNNVLLDEANIWL
                     YPSRIMDYPNLAIGEFGYDPDSDPIESSSPADFTRFGVHFKIGRDNPWVLGAYFDNLP
                     ASEPENLFDDGNLVEFDTLFDDGWNNRRINLFYGNSLGNINFGARLGLLHSSVSADMT
                     DNEAKEGFSYYDLTLGLTAPDGGWDVAGNIALGSFTDDNEDGDAENEKDGFYDLSLQG
                     RFFYQVNPSYTIIPHAGLAYSKRGIVNYTNDDDVDSEDYSISTSRTGLQLGVGLNYTP
                     ATNVLAVADFGLGYNKVKEEFDTTDTYVTDPDLPEYTERNKTDVWLPYFKLGLDADVF
                     KWLDIRMGAVSIWNRQSDETKLAEDVKYKANYADNSAYLGFGFHWGRFHVDAWTDPEL
                     FLNGFNFLSGADSGEMNFQLSAVYEMM"
     gene            complement(98158..98847)
                     /locus_tag="PKM94_00350"
     CDS             complement(98158..98847)
                     /locus_tag="PKM94_00350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOD65116.1"
                     /translation="MTKPILLIQNVAAEGPGTITEWLESRGRPFMLVKAYEGEALPDP
                     DAIEGGICLGCPHSAARYHEHVYLKALFAWLARVVREDRPYLGICFGGQLLARVLGAP
                     VEEGNAREIGTYMAKLTEAGAADPLFAGFPREFAMFHWHNDTFRTPFGGVHLAEDEVW
                     AHQAFRRNRQVGLQFHLEPRACDVVRWCEAYPDELAVLGKTKEEVVAEFEGAYETIRG
                     TNFRLLDNWLG"
     gene            complement(98970..99869)
                     /gene="speE"
                     /locus_tag="PKM94_00355"
     CDS             complement(98970..99869)
                     /gene="speE"
                     /locus_tag="PKM94_00355"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOD65117.1"
                     /translation="MGQKKTGGEVYIAEEGMKDLWNVWYSELHEGQSGLTIKVDRMVE
                     SVQSEYQRIDVLETFDFGKMLVLYGSLMVCDRDYNAYNEMLAHVPLFVHPRPRQVLII
                     GGGDCGCLTEALKHPGVERCTMCEIDRMVVEVSKRHFPALTAGLADRRAQVVYQDGKR
                     YIEEATERFDVVLLDLSDPIGPAADLFQKSFHQTVHERLADDGIMVAQSESPFYNRDT
                     VHQMYRNLREIFPIVRMYTCFMPIYPSALWSFAFCSKKYDPIRDFRADDHARLRPTTK
                     YYNAEVHHAAFALPQFVKEIAAD"
     gene            complement(99889..101061)
                     /locus_tag="PKM94_00360"
     CDS             complement(99889..101061)
                     /locus_tag="PKM94_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOD65118.1"
                     /translation="MTPHVDVSVMEQRARATGGDQGLLRELLAGARADTPALLVSRSA
                     IVRNIQALRRALPRVDIHYAVKPNNHEVFIDEVFGQGGNFDVCSAGEIDLVLRRTSLD
                     PATLIHSHPIKSVPEFDYAVGRGLELFVVDNPAEVPKFTRYAEKKLKVMIRYRTAVNT
                     SAVVNLQYKYGCMPDEVLPLARLVRETGHDFYGLCFHVGSQCVYSDNYVNAIATAHAL
                     IRALDEEGFDTRLLDIGGGFPVEYVEPVPAIAAFAAPIAKALDDHIRPGIRIVSEPGR
                     FIAATPVTLVTSVVGRSYRDGKIWYYLDDGLYSTFSGIVFDHCQYPVVTNKKGEQFLS
                     VLAGPTCDSFDVMYDGLMIPLHEVGDRIVFTATGAYCSVSGSNFNALRRPEYLVID"
     gene            complement(101078..101500)
                     /gene="speD"
                     /locus_tag="PKM94_00365"
     CDS             complement(101078..101500)
                     /gene="speD"
                     /locus_tag="PKM94_00365"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOD65119.1"
                     /translation="MKILGRHLVVELSECNQEKLNDLSFLEECLDEAVRRSGATKVRS
                     VFHRYNPQGVSGVVVIAESHISIHTWPEYGYAAVDFFTCGESVDPHRALAHVKGELEA
                     GSAQVSEFKRGIPSHADEIIDHKPSSRRRPTQAAAALR"
     gene            complement(101537..102079)
                     /locus_tag="PKM94_00370"
     CDS             complement(101537..102079)
                     /locus_tag="PKM94_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOD65120.1"
                     /translation="MKVTIGEKIKALRLASDLTQAELASRARLTRGFISQIENDQSSI
                     NLDSLADILEALGVGLGEFFSDVEKRRVVFRPSERVNVEGKGARRFELLMPGSTNNLM
                     DPILLELGPGEFLELRDPMPGEQFGFVLAGTVTLRIDGKSFKVPREHCFYFKSDQRHQ
                     IGNAGERAAKLLWVTTPPHM"
     gene            102826..104265
                     /gene="lpdA"
                     /locus_tag="PKM94_00375"
     CDS             102826..104265
                     /gene="lpdA"
                     /locus_tag="PKM94_00375"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOD65121.1"
                     /translation="MPQYRRPRSKPGDSVIEKFNIVIIGSGPGGYTAAIRAAQKGARV
                     AVVELGELGGVCLNVGCIPSKALIASANEYRRMRDAELWGIRLASPPVYDWTAMRARK
                     DRIVKLMTGGIAQLFKSHGVTHLAGYGRITGANQVTVVDDQGNESKLKTDTVIIATGS
                     RSRNLPGLTVDGRRILNSTHMLELDHLPQSLLVIGAGVIGCEWACMAALLDVRVTVVE
                     MLDQALPLEDAGSAKLFERELKKLKIDLHTKTTVESLTPAGETVRANLSGGQAVEAHQ
                     VLVAVGRAYNTEDIGLEEVGVKVNKNGSIPTGDDMRTNIPNIFAIGDVRGEILLAYTA
                     VHDGAVAVTNALGETTTKDYRGVPSVIFTHPEVASVGLSEARAAQEHDLAIGRFPLRV
                     LGKSHAENEIAGEVKVIADKKTDRLLGVHIVGAHATEVIHTAALAIRNGLTARELGNL
                     IFGHPVISEALMEAAHDVHGMSVHLAKKR"
     gene            105097..105170
                     /locus_tag="PKM94_00380"
     tRNA            105097..105170
                     /locus_tag="PKM94_00380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105131..105133,aa:Met,seq:cat)
     gene            105350..106411
                     /locus_tag="PKM94_00385"
     CDS             105350..106411
                     /locus_tag="PKM94_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOD65122.1"
                     /translation="MPGHIARTTGRIAALRTAGWIAAARSTGRIAAVRTAGRSTAART
                     VGWIAAAAAAAVLGCSGADDTAKVKQNPASEGAVTIGVSLLTRTHPFYQELEEGLKEA
                     AGAAGFQVRITAGEFDVARQKDQLQDFLTQRVDAIIVCPCDSRSIGTAIKAANEAGIP
                     VFTADIACLAEGVAIVTHVASDNVEGGRLAARALAEAIHGAGAVAIIDHPEVESVIQR
                     VKGFEEEIAKYPDITIVAKLSGHGVKDQAFRTAEDILQAHPDLAGFFGINDDSALGAL
                     AAIEKAGKLGRVKIVGFDAVPEARQAIEAGKIHADVIQQPRKIGQRTVEAVQTYLSGG
                     AVEPQILIPCDLFAKPAAQ"
     gene            106433..107941
                     /locus_tag="PKM94_00390"
     CDS             106433..107941
                     /locus_tag="PKM94_00390"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOD65123.1"
                     /translation="MDSSSETPAPGGHSLLEMSGIEKSFPGVRALRAASFALRAGEIH
                     ALVGENGAGKSTLIKILTGVHRRDGGEIRLLGQPAEFRRPLEAQRAGIATIYQEFTLV
                     PSLSVAANIFLGHEAARRGLIDHGRERRAAAALLDRLGAALDPSALVSGLTVAQQQIV
                     EVARALAREARILVMDEPTAALAPHEVAHLFAILRDLAGRGMGIVFISHRLDEVAAIA
                     DRITVMRDGATIAVRRAGEFSRAELIELMVGRPLDQEYPKAPAPIGPVHFEVRGLSGG
                     FIEDVSFAVRRGEVLGLAGLMGAGRTEVARLIFGADRKRRGEIRLGGRPLEIASPRAA
                     IGAGVCLLTEDRRRQGLVLGASARDNFALPNLGSWSRLAWIDRRREQERFAERTAGLN
                     IRLSHPEQRAEELSGGNQQKLLVARWLETNSEVVIFDEPTRGIDVGAKYEMYVLIGRL
                     TALGKCVIVISSELPELLGICDRILVLRRGRVAGEVAEAARATQEDLMALAV"
     gene            107961..109400
                     /locus_tag="PKM94_00395"
     CDS             107961..109400
                     /locus_tag="PKM94_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD65124.1"
                     /translation="MENSRGKKHRREAGAARLRQAFSDYGMALVLAALCALFSLLTIS
                     EQHPTGAAAGDNLCAQLAAGTAPGGAVVVIGQATGDDSAMVRTLVGRLANAGRTVRPP
                     VFGSPAEVRARLEALDSAGQAAPLIVTSHAYYAAVTMVAAQVAGFRAAKVCEPSPHRW
                     PTFLLADNLRNVANQISVIAIIAVGMTLVIITAGIDLSVGSLIALSAVVSAWLIGRAG
                     GDQAGPAAMILASLGGILLAGGVGAFSGFMVTGFRIPPFIATLAMMQVAAGLGYIISQ
                     GKPIYQLPDSFVWLGRGVDPVFHIPNAVILMVLLYAAAHVVMTRTTYGRYVYAVGGNP
                     EAAHLAGIRVKAVLFSVYVICGLLAGLGGVVMASQLKSGAPTYGLTYELYVIAAVVVG
                     GTSLMGGEGRILGTLIGAFIIAVIQNGMNLINVESYTQKVILGLVILAAVLADRLKQR
                     RLARRRPGPSARRDPKPDNTATANTEQRP"
     gene            109397..>111821
                     /locus_tag="PKM94_00400"
     CDS             109397..>111821
                     /locus_tag="PKM94_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOD65125.1"
                     /translation="MSIKTALLALLLAAVGTPAGSQPEQAAPPEQRPTLQVIGTAHLD
                     TQWRWTIRNSIDEYIPDTFRRNLALMAQYPQYVFSFEGAFRYMLMEEYYREDFERIRP
                     YVQSGQWRVTGSWVDAVDVNIPSFESLVRQTLYGNGYFRKEFGRTSRDIFLPDCFGFG
                     YALPSIARHCGLRSFSTQKLGWGCWVGIPFDIGVWRGVDGATLLCGVNPGEYVSRITG
                     DLSRDTTWLGAARRQGEKSGLFAAYRYFGTGDTGGSPDSASVDRLVASMQSDGPLRVV
                     SAGADDLVDLLAGVDTARLPHFDGELVMTRHGAGCYTSQAAMKRWNRRNELLAEAAER
                     AAVIAARRGGSFYPRGLLRDTWIRFLWHQFHDDLTGTSIPEAYEFSWNDELLCQNRFA
                     ALLTGAVEATAGALDTRTQGIPLVVFNPLGFDREDVVEAAVHFGDRKTRFVRVFGPDG
                     REAPADAVPMAGDSLRLRFLARVPSVGYAVFDARPADAPCPLATGLRVSPRELENERY
                     RVGLNEKGEVASIYDKKLGRELLGAPVRYELLEDTPHNWPAWEVDYEDVMAPARDEWA
                     GDPVIAVVEEGCARVAVSIERRTASSTLRTIVSLAAGEAGERVEFQAAIDWYERERML
                     KLAVSPATANETVTYDLGLGVIERGRNSEKRYEVPGHQWADLAAPAGDWGVAVLNDCR
                     YGWDHPDSATVRLTLVRTPGVGKGWDWVGDQRSQDLGRHLLGFALQGHAGDWRSGGVV
                     RQGARFNQPLQAFRAQPHEGELGKTYSLVRAEETPARGGAATEAPGVMIAAVKMAEEC
                     DEIIIRVRET"
BASE COUNT        19761 a        38529 c        35067 g        18464 t
ORIGIN      
        1 gctcacccgc cgcatgctgc tgctaaagta gcacgcgccg gcgcccaaga cgccgatccg
       61 cagaatctcg ttgtcgcccg gtgtcgggcg gcctatattg cccgccgaca ccatgaatcg
      121 accggcagcc cgcaggaagg ctctcctgta ggcttcgccg gaggtcgcgc gggggttcgc
      181 acgcaaggag gttacgcatg agtcgcatag gtatgagcgc agtcctcggg ctcttgctgg
      241 cgggcggtct cgccgcatca tcggcagacc ggccctccgc gctttccaga cgcatgtctg
      301 cgggctgtcc cacccgcgat acccggtaca gcccaaggat cgaaacgtat ctcactcccg
      361 acggtcggtt ggacgcggat gcggcccggc gctcctccta ctcggggccg ctcgacgttg
      421 aaggattcgt cccgaccatc gatcccgcga ccggcgcacc ggtctttcgc gcggctttgc
      481 cgctcgcgcc ggccgactac cccgacgaca tctactggga caaccgcatc tctccctccg
      541 tgcccggcgt ttccggtaca gtgcgggcag ttgccgtgta caacgggcaa ctcatcgtgg
      601 gcggaagctt tgaggtagcc ggcggtgtat tggcccacaa tattgcgtcc tgggatggca
      661 cctcctggtc cgctcttgga tccggagcca acgattgggt ccgctcactt acggtgtatg
      721 acggttctct gatcgccggc ggagattttg acacggccgg cggaatacaa gcgaatcgca
      781 tcgccgcgtg gaacggcttg tcgtggtcgg ccctcggagc ggggatggat cgctcggtga
      841 aggcgttggc ggcctacgac ggtcgcctgg tcgcgggcgg caactttgac accgccggcg
      901 cggttgccgc gcgccgcatt gccgcgtgga atggctcatc ctggtcccct cttggatccg
      961 gcatgaacgg gaacgtggaa gccgtgactg tctgggatca ccggttgatt gccgggggat
     1021 ggttcacgac agcgggcggc atcagcgccg cccgagtcgc cgcctgggac ggttcctcct
     1081 gggcgccgct ggcgtccggg atcgccgatg gaggcgtcta cgcgctggtc gaatacaacg
     1141 gcaacctgat cgcgggggga tatttcacca ctgcgggcgg aaccggcgcc aaccatatcg
     1201 cctcctggga cggccggaca tggagtccgc tcggaacggg gatgaacggt tctgtgtatt
     1261 cggtgggcgt ctatgacggc catctcgttg cgggcggatg gtacgcaacg gccggaggga
     1321 ccgccgccga gaacatcgcc gcctggaacg gttccgcctg gtccgcactg ggatccggag
     1381 ccggcagcgg ggtctatgct gtgactccct acggctctgc gctggcggca gggggagctt
     1441 tcggggttgc gggcgacata cgggcggtcg ggatcgccgc ctggaacggc gccgtgtggg
     1501 cggccttagg ggccgggacg gatgaccttg tctacgccct caccctctac aacgggcgcg
     1561 tcatcgccgg tggacgattc acttcgatcg gcggggttca ggccaaccgc atagccgcct
     1621 gggacggttc ctcgtggtct ccgttaggag tcgggttgaa cgatgatgtc tacgccctga
     1681 ctgtgtacga cggccgcctg actgccgggg gtcgcttcac ggcttccggc gagcagggtg
     1741 tgagccgcgt ggccgcttgg gatggttccg cctggtcccc tgtcggaccc ggcgtgaacg
     1801 gcgacggcgt gtacgccctg acagagtaca acggtgactt gattgcgggc ggatggttcg
     1861 atacggcagg gtgggtcgtc gccgacaaca tcgccgcctg ggacggctcc tcatggcgcc
     1921 cgctgggatc ggggatggcg gacggagacg tctacgcact ggtcgaatac aacggcgacc
     1981 tcatcgccgg tggatggttt tactcggcag gggatactac tgccccgtat gtcgccgcgt
     2041 ggaacggttc cacgtggtcg ccgctcgtgt cgccggacga ggaattcaac cccgtcaacg
     2101 ctctgacggt ctatgacgga agactgatcg ccgctctgga tgtgggggcc tataagagtg
     2161 acgcacagac cgcctacaat gggatcgccg cgtgggacgg cacggcctgg tcgtggctgg
     2221 gaacgggcat cgccgatgac aacgcctcag tcgatgccgt ggccgtatat ctcgggcgtt
     2281 tgatcgccgg cgggcgcttc atcacggcgg acgatccggc ggcctacttc attgcctcgt
     2341 gggatggccg ggcctggtcg gccatgggat cagggctcag cggctcggca gcgtccttca
     2401 ccgtcgccaa cgaccgcctc tttgtcgggg gtcgcttcac gatagcggga ggaaaggtcg
     2461 cagctttcct tgccgaatgg accaaaggcg tggataccga tagcgacggc atcgacgatg
     2521 atctcgacaa ctgcccgacc acctacaacc ccgaccaggc cgatcgcgat gtcgatggcc
     2581 tcggggatgc gtgcgatacg ggtccggagc tggcgttgag cccggagacg gtggatattg
     2641 gttgggagtt gacgcgcggg gagttcgtgg tgaagaacgc ggggtacggg acgctggagt
     2701 tccggctcga cacgctgccg gagtggtgca cggcgaggcc ggtgtcgggt tcgctgggag
     2761 cgggggggga ggcggtgatc gaggtggagg tggaccgtcg ggggacgctg atgggtgacc
     2821 atgcgggcgt ggtggcgatc agcagcaacg ggggcgcggg cggggtgttg gtgcgtatgt
     2881 acacgccgat cgcgccgctg gtggggtctg acagcgtggg gatcgaggtg ggggaggaga
     2941 tacggatccc gttcaacggc ctggcggagg cggggtcgct gttgtcgcgg ctgcaggtgg
     3001 tgtcggacgc gggggaactg gcgtatggtg tggcggtgga tacggagtac gtggggctgc
     3061 ggattccggt cacggtggtg tacatctacc cggacgaggg ttggtcgttt ccggagctgg
     3121 cggggattcg ggtgatgttt gcggattcga ttgttgatgc gcacggagtt ccggggcctc
     3181 aggggtacgc gttcacgtat gtgaacggtg cggtggtgtg gccgggggac acggatgggg
     3241 acggggtggt ggacgagcgg gacattttgc cgatcgggtt gtactacggc tcgggggggc
     3301 cgggccgggg ggagggccag gggacggagt ggggtccgta ctcggtggcg gtgttggcgg
     3361 agggggtggt gtggcgtccg ctgcgggcgg tgtacgcgga tgcagacgga tcgggggagg
     3421 tggacgcgct ggatgtgtgc gcgatcacgg agaactggcg ggcgagggcg gacgggtgga
     3481 agcggggtgt gggagagggg gccggggtgg aggggttgcc gaaggagttg agtccggggg
     3541 tggcggcggc gctgcgggcg gcgctggcgg agtgtccgga gggcgcgggg aaggcggcgc
     3601 tggcggaggt gctggggggg atcggggagc cggtgcttcc gggggaggtg acgttgtacc
     3661 agaactatcc gaatccgttt aatccgtcga cggtggtgga gttttatctt ccggcggcgg
     3721 gggagatccg ggtgggagtg tggaacgtcc tgggtcagga ggtggcggag ttgtaccggg
     3781 ggtggcacgc gtcggggtat gggcggctgg agtggtcggg gcggacacgg ggtggcgggg
     3841 aggcggcggc gggcctgtac ttttaccggc tgacggcggg cgaccgaacg ctcacccgcc
     3901 gcatgctgct gctaaagtag cacgcgccgg cgcccaagac gccggcggtc gcgcaacttt
     3961 cgactgtcac gtgcggggcc ggcggcaacc gccgccctct cgctacatcg gaccggggga
     4021 gccggcatcg atgtagcagt catccgtgta gagaaccgcg gtcacctgcg tgctgtcgat
     4081 cgccgtgccg tcggtgagcg tcatcgaggt cggagccagc cagatggatg tcgaggcggc
     4141 cgcgggggcg ccgacgccaa gcgattggac gtgcacatcg acgagcggcc ccctggcggc
     4201 ggcatcgacg agcgctctcc ccggcacagt ctgcgtgagc gccaccgccc agtagcccag
     4261 accggtctgc acctgcacaa attgcggggg caagaacaga acttcgccgc cggccaccag
     4321 ggccgccaga gcgggggacg ggccgacgct gtcgatgcgc accaggtcgt tccgatagta
     4381 cacgcgaaaa gcgatcccgt acagcgccgg ctgccggtag aagcaggcct ggatgaccgt
     4441 atcgagcacc gccccggagt cgagaggagc gatgtagcgc ggcacgaact tgagcatcgg
     4501 caccgacggc gtgagcggaa ggtcgatgac ggtcgtcagg tcgggtgcga tctcctgcac
     4561 ctggcgcccg ccgtacacct ccacgccggc gtcgttgagg atgctcacga caaattccag
     4621 cggctccccg gaggggaggt tctcgactgt gaccgtgacc accgaggcgg tcaggtcgta
     4681 gctggtgtcg gccgccagaa gcacgccgtc cctgacccgc gcgactgtga ggatgaaagc
     4741 ctgaaacgac tcctggaggg ccgaggtatt cttctgcagt tggacagcca cggtgacggt
     4801 gcggccgggc tgccggccgg tgacggtgtc ggcgcagccg ccggcgaaag ccgccaggac
     4861 ggcgccggcc agaagagccg ccccggtact ggtccatatc ctgttcatag ttcttcccac
     4921 gaaatccccc ttcacatcgc ggcctggagg ctcaccgagg cctgccagcc gctcagatcg
     4981 atggccccgc cgtcggcgct cccggtgagg taccggaggt ccacatcgag gctgccgtga
     5041 gcggcgattt tcctgcttgc ctccacgccc agccggacca ccgtgctcgc gccgaattcg
     5101 tcatcggccg cgatcaagcg gaggccggcc gccggcccga gaccccaccc ctccgatacg
     5161 gcataccggg cgcggccgga gagatggaac tcgttgccgt agagccggcg gttcagcgat
     5221 tcctcgtact ggtcgtagag cgcgctctct cccctcagaa ggtaccgggt caacaggaga
     5281 aggctgagcc ggtccagttg cgtctggttc gccaggcgga aatcgaggtc ggtgctctgg
     5341 cggaacatct tggcgccctc gcgcgtgtcg gtcgtgcggt agctgagcgt ggccgccgcc
     5401 gaccagaccg cgcggccggc cttgaggtcg gccccgaggt tggcggacag cacgtcgccc
     5461 gggttgtaat cgtcgatccc ctcgtacggc gagtaggcgc cggtgtagga gtaggagacg
     5521 ccgccggcca ccgtggcgcg gccgtagttg cgggcgaacc ccgccagggc gttcagcccg
     5581 agaccctcgc cgaaccgccg caccgggaag tccaggaaat tctcggacag cacctgcacg
     5641 atcatccgct catcggcaaa gcgcagctcc ttcttgccgg tggggaggct gaggccgatg
     5701 cttagcagga tttggtcgtt gctcagcgac cggctcacct gcaggcgaag gtcgccgagg
     5761 ccggagacgt cggccgcggc ctccggcgat tcgagatccc cggccgcggt ggggaggaag
     5821 aagcgggcct ccaggttgtc ccgcagcggg aggaaaccgc tcactatcag gaggctctgc
     5881 gcgatgctgg tcgtgccggc cccggtcgaa tcgagatccc agctcgtgta cacgaacgaa
     5941 gcgcgcccgg aggcgggctg gccgtagatc acctggcact cggccgccga ggccaggccc
     6001 gccaccgcca gcagcagcgc ggcccagcgc cgcagtgcat gactatttcc catcgaggtt
     6061 cccccttacc tgggccgcgc cgaccccggc cggcaccggc ggctcctccg aatccgacgt
     6121 cccgtcctgt tcgccgtcgg ccggaacgat cccgagatcg tcgtggagcc cctgcaggct
     6181 ggccccgaga tcgtcggggg cgccgccgag gaaatcctgc ctgaccgcgg actccacctg
     6241 gccggcgctc acgtccccca tgttctgcag gctcatcagg gtgttcgtgt tgtccagttc
     6301 cgtccgggcc tgggaaaagg aaccgtcgtg cttgagcgcc tgctggaact ccacctgcgc
     6361 cgccttgtag tacccttcgc cctggtactg caggccccgg cagtaggcga ggaaggccag
     6421 cgatgattcg gtcggcacat catccagccg ggcccgctcc tcgggcgtca gcgtgacgtg
     6481 gagcgagtcc agcagagaga agacgaatct tttctggaag cggtagaact ggtcgagggc
     6541 atcctcggtc ggggcggacg gagtcggcgt ggcccgcacg acatccacca gcgccgcgtc
     6601 gaggcggatc ttgtcgccga tgctcatgag ttcgccggtg acgacggtgt gggcccccag
     6661 caggtagccc ttctgcaccg cgctcagcga gtcggcgaca cccatgtcgc ggagccgctg
     6721 ctcggccagc agcgtctcga ccttcagccg ttcgatcgag cggatcgagg agaccttggc
     6781 gagatcggcc gcgaccagcg ccgacagccc gcgcgccacc ggggcgacct cggggtcgag
     6841 gcgggcgccg ccgaaattca ggaccgccac cgagttctcc ggaatcgcgc tcgcatccaa
     6901 ttggccctcc ccggccacca cctggggaag ccggttccgc agctcctcct gcaggagctg
     6961 atcgagacgc ttgcggacgg tcgcggccga gcggccggtc gggtaccggt cgagggcggc
     7021 gatgtagtag ccgacggccg aatcgggata gcccttgttc tcctgaatca ggccgaggta
     7081 gacgtaggcc gcgggatcgg gggccggctg gttgacggcg ttcttgaagg ccacgtccgc
     7141 ttccgccgcg cggccctcct tgtagtacgc gatccccacc tcccgccagg ccgccgcatt
     7201 ggtcggattg accgacagct ccttgaagag acattcgcgg gcctgctcgt agcgggcctg
     7261 atccgtcagc ttccggccct gggtgaccag gttcggagcg cacgaaacca tcagcagggc
     7321 gccgaggagc gcgaggccca ttcgcatgcg catagtgtct tgcctcactt cggttgcgaa
     7381 agacgggttc atccggacgg cgcggctacg cggccagatg gacgtagaca ttcatcttgg
     7441 tctcggcctc cttgaacccg gggtcgagtt caaaggcctt cttgaaatgc tcgtaggcca
     7501 gcttgtagtc gtgcttcttc tcgtactcga cgcccttggc gaaagcgacc gtggcgtcgt
     7561 acgatccggt gccgccctcc tcgatcagct tcatgtcgtc cttcgacagc ttgacatcga
     7621 gcgcctggca cagttcctgc acgaggtcct tgacgatctg gaagtacttg tcggggttcc
     7681 cctcggccga caccgaggcg atgatttccg aggtctcgac gttgacggcg cgggcctgga
     7741 ccatcgtgtg gctgcggtcc agttgcgtga aggagccgaa caccatcacg tgggcctgca
     7801 ggatcttgcc ggcctggatc gcggtcgcct gatcgacggc gccgccggcc gccagcttct
     7861 gctcattaat gatgtagtcg aatttctcgc gctccaccac ctgcaggctg cccagcttct
     7921 tgaactcctc ctgaaacatc aggccgagcg ctgaaccgag cgacccgagt tcatcgaggt
     7981 acttctggac cgagaagttg tcgaagtacc agacggcgac ggtctgcggt tcggtcgggc
     8041 ccgtcgactt gcaggtgagc gcgccgagcg gactcaccac cgagtaccac cggttcttca
     8101 gatcctgcgg ccagacatcg cgggggagcc gcaccaggca ggggctgatg gcggcgatct
     8161 tctgcaggca ggtgtacgac cgctcgaggt agtcctcccc catcatgcgg tacacgaggc
     8221 tctgcatttc gaacacggcg atgctgtcat aggccgtgag ggcgggccgg gccagcagct
     8281 tgtccaggcg gtcgaggcat tgctgccagt cgccgcgggc atagtgctgg cgcacgacct
     8341 ccagctcgcc ggggacatcg gccggtgtct gggccacaag tgacaccgcc gggagcgcgg
     8401 cggccgacaa aaggagagct gccacgaggc gcatgcgaaa ccgactcatc ggaatctcct
     8461 tgtggaattt tgcgattttc ctttttgaac ccgcttcatg atacggcatc cccggaccgc
     8521 agtaaagacc ttttttggcc ggctcactcc accgccaccg agtcgccggt gaaatcatac
     8581 atgaaccacg ccgtgtcgcc gtgctgaacg atgcgcccca cctcgggcga ggacgcgacg
     8641 tcccctttct tcacctgaac gcggtattcg cccaccggca gcttgaagaa ctggggggtg
     8701 gatccccgcg gccggccgtt gaggtagatc tcgttcccca cgggatcgga cccgacaaac
     8761 agcacgcccg aatcggcggg gagcgcgaat tcgaacgaga cccgggccgt gcgcccgccg
     8821 accacgcgga ccggctgttg ctgcgtgctc ggcgccgctc ccggatgatt cgcttcgagc
     8881 cggtagtcgc cggccgcgag caccgtgtcc caggaggtaa cgcgctgcgc aacaacggcg
     8941 ttatcgagct tcaagcgaac cggccggttt gccgtgacct tcagtgtgcc gctggttgcc
     9001 gccggcccgg ccgccaccgc gaacctgccc ggcgccgagt aggcactggc cagaccctcc
     9061 cggctgaccg cccgcacccg ccagtacatc acctccgccg cgaaactcgt gtcgaggatc
     9121 cgaaacgtgt cgctgaggtt ttcgtacgtg gccgaattca gaaagtccgc attgtcggca
     9181 atctgaacgc ggtagtgcac ggttgaatcg ccggctgagc tccacaccag ccgaaccgcc
     9241 gcgcccgcct ccagccgccc gccgtccggc ggcgccgccg gcaccggaac cggcgggggt
     9301 tccagggcgg ctgtcgtatc cggtccggac ggttggccgc gtccgaagaa aaggtaggcg
     9361 gcgactgcgg ccgcgaccag cacgcccgcc gcggcccact tcacccccgg gcgcgccggc
     9421 ttccgcggcg gcgggggcgg cggcgtcggc gtcgcatagc gaaacggcag cgtcacaccc
     9481 gacgcggaca ctagcgcctc tttcatctcg agggccgtct ggtatcgctt ggccggatcc
     9541 cggtggatgg ctttcaggac gaccgcggtc agcgccggcg agacctcccg gttgaaagcg
     9601 tgcggcgact tcgggtcctg aaacagcttg gcgtcgcgga tagcgaacag atcgccgccg
     9661 gcaaacggaa gcgtcccggt caggcattta tagagcgtca ttccgaccgc gtagatgtcg
     9721 gtcaggtcga aacgggtgtc gtcgcttgcg gaaaactgct cgggggccat gtactcgggc
     9781 gtaccgcagc agccgcccac cgcggtcaac gacggatcgg tcttggcctt ggcgatgccc
     9841 agatcgatga tccgcaccgc gccgttcttg tcccgcatga tattgctcgg cttgatgtcg
     9901 cggtggtaga tccccttgcg gtgcgcgaac gccagcacat cgagcgtctc cacggcgatg
     9961 aggaccgcct cacgcacggg caggtggccg ctggatttga tgaggtcctt gaccgtgacg
    10021 ccgtcgatga actccatcac gatgatcagg tccccctccc actgaaaaaa acccttgatc
    10081 cggcagacgc gggagtcttc ctccagcagc gccaacttgt cggcttcctg gcggaagcgc
    10141 tcggcatgct gcggatcttt caggactttg aggataacct tgagactggg tacttcccgg
    10201 tggacagcga ggtagacgcg ggccatcccg ccggcccctc gctcttcgat tactctgtag
    10261 tcgccgatca gttgctcgtt cattcgctgg cgccccggtt ccggcccttc ggccgcgtcg
    10321 ttgatggcga gtcaaattac ggacgcccgg ggcggaatgc aaggcgaatt cggccgcggc
    10381 tccccggctg cgccgggccg gcggtcgatg ccccccgcgc cgcggacggc gcaaaacaat
    10441 gattgacggc cgcgcgcccg cgcctttatt attgtagaca gcagcgatcg aaaaaaccat
    10501 gtgcccgccg ccgaaccggg cggccggcac catgggcgca gcggagcggc ggcgaggcga
    10561 tcgcgacctc ggccgccctt tccgaacagg gagatctgag catgggcaag ccggcagcgc
    10621 ggatgggtga tatgacggtc cacggcggag ccatcgtcgt cggcgaaccg actgtcctca
    10681 tcggcggcct cccggccgcg cgcgtcggcg acatgcacac ctgcccgatg gttaacccgg
    10741 gggccccgcc cccgccccac gtgggcggcc ccatcatgcc ccccggcgcc gtcaccgtcc
    10801 tcatcggcgg caaaccggcg gcctgtgtcg gcgacatggc cacctgttcc ggcccgcccg
    10861 atgcgattgc accccccggt tgcccgaccg tgctcatcgg ccccggcggc ggcggcggag
    10921 gaggcggcgg cggcgccggc agcggcgccg cctcgggaac cgccgagacc gcggccggcg
    10981 aaggcggcgg cgccggcgaa gccgccgagc aagaacccga gggacacttc ctcgacgtca
    11041 agtacgtgga caaagccggc aaaccgatca ccggcgtcgg gtacgtcgtc aagggccccg
    11101 acggcaagac ggactccggc ctcctggccg gccgcatccg ccgcgacgga gtggatgccg
    11161 gaagttacga gatcggcctg cgctgcatct ccaaggccga gtggtcggtc gagcgggcca
    11221 aggtggggga tacggtgacc ctgcgcgccg tgtgcgccgg actcgaatcc ggaaccaagg
    11281 ccgctctgaa aatattcgtg cgcgactaca acgtccccga ccgcgtggtg gccgagcttc
    11341 cggccgaggt cgccaacgat gccatcgagg cggactgggt cttcgctgtc gacgaaaaga
    11401 tcctcaggct gcagtcatcg cagcaggccc ggagcggcta ttccagtccg cggttctact
    11461 ttgtcgctga ggccgagggc tgcgctgccc gctcgccctt cctcttctac agcgactggc
    11521 tcgaaatccg gcttgccgac agccagggaa aagcggccgc cggaaaagcc tacaaagtcg
    11581 tcttccccaa cggagagatc cgcgagggga cgctggacag cgacggcaaa gcccggttgg
    11641 aaaacgtgcc gccgggaaag gcgcagatat tctttgaacc gcaggcgtga ccacgccggt
    11701 tgcccgcgac tgcaaccggt ctcgcctgcc gacgtcaaca tggtcgcgga cccgttatgc
    11761 gagggatggc cggtgatcga tgaaatgggg aaaaccaaca agcggttctc gatgaggctc
    11821 gccgagggct gggaggacca gaccgtgcac ctgttcgtcg gccccgccga gcgcggcatg
    11881 aaccaccacc tgacgatcgc ggtcgacgac atccccctgg cggagacggc ccaggattac
    11941 gccgagtacc gcgtcgagca gtttctggac accctgccgg cggccgaggt gctcaaagag
    12001 gagccgctgg cgctcggtga ggatctcgac gcctacgagg tcgtgatcaa attcgtgccc
    12061 tcggaggccg gaccccggtt ccgccgcctc gtgttcgtgc tccacgagaa caccgccttc
    12121 accttcgcgg cggatttcac caaagtgacc cggaatacgc tcggggcgca gatgatcgag
    12181 atgatcaaga cattccggcc ggaggccggc gcctgaaccc gcgaggctgt atgaccgccc
    12241 tgaaaagcac caaagccacc gacgccgagc acgaagtcaa gctcgaaccg aggctcatct
    12301 cgctcagttg gcgctccgga aaagcgcgcg ccggaggaat cgcccggttc gaggtggtga
    12361 ccgagttcgt cggcaacggc gcgaaagtga aagtcaccgg caaaagcgaa aaaggaaaaa
    12421 aactgggcgc cgtcgacgcc ctcatgcgca ggaaccgcct caccggacaa ttcgacgtcc
    12481 ccgccgatat cgacctcgac gaccgcgtgt tcttcgaggt caggctcccc ggcaacgatc
    12541 tcgaggggac ctccgacctg atcccggcag cccccccggt caaagtcaac agcctccggt
    12601 ggagctgcga cgaagcccgc cgggacgatg aggtcgcgct gtccgcattt gtcgacgggg
    12661 tcgatgaggc ggaagaagtg gcgctgacga tctacgagtt cgaccgcgac ggagcgcacg
    12721 acaaaatcac cgaactcccc ggccaggtgg tcgacggccg ggtggagaag aagtggcggt
    12781 tcgagtacca cgaagatgtc gacgagatcc ccacccagga ggagatggag cggtacggcg
    12841 gcgcctacaa cccgcccgag tacttcttca cgatcaccgt cgatggcgtc gagttcggca
    12901 agaagcgcgc ctccccccac ctgctgactt tccgcgactg gatcgagctg agtctgtgcg
    12961 actcctccgg ccggccgctg gccaacgagg attacgaact gctcctcgcc gacgggacca
    13021 agcggacggg gacgctcgat ggggagggca aggcgaaggt cgacggcgtg ccgcccggcc
    13081 ggtttcagct caagctggcc aacgttcgcg gcagccttgc ccacggccgt ccggcggtcc
    13141 agcagacttg cccgaaagcc cgggcgatcg ccaaagtgga aggaccctcg cgcatccgcg
    13201 tcggcggacg ggcggagtac acggccaagc gtgcgcaaaa cgccgaccaa tcggcgccgg
    13261 tcaagtggcg cgtcagcgat atcgagtcgg aggaggttct ggaggtcgaa accgaaatcc
    13321 agcaagacac gctgatcctg ccgtgtatcc cggactcgtt caaggggaaa acggtcaagg
    13381 tcgaggcctg gcaggaggag ccggaggacg gcgcgacttt ctcctcgctc gtcaggtacg
    13441 acgaagtggg ggagtacatc gcccgctgtc gcaaactcga gaacgccttt ccggactgga
    13501 cgggggaggc ggtcctcaac gccctgcggc ggctggcggg gtgcgacgat aaacggtttc
    13561 agcggatgtt cggggtggcg gcggccgata acctgcagga aatgtgcgat tccagacgac
    13621 tgccctccgg ggttgccgcc gacgacatca agtggctgat agaacactcc cgacaccgag
    13681 agaagatcga ccctcctgat gaccggtcag gagcaatgaa gctgaaagaa cagccccaac
    13741 agaaactgga agcagacttg cgcggctgcg ccaccgactg cctcggatac ccgctggcga
    13801 tgggccacgt gctgtgcggc atctcggccg gccagcatcg gaatacctcc gtgcggccac
    13861 atttccttgt cgttccgggc gcgctcggat ggcacgtgga caacctgtac gccgtcaccg
    13921 tagccggcga cctcggccaa tcggcggtgt gcgtcttcct gggtgataag agtgttttga
    13981 tcggcgacga tcgggccacg accgaggcca cctacgatga gttgcgcggc gatgtcgacg
    14041 gttttgtcct cggccaccac ttgaagtcgc tactgaacgg acgttcactg gacaaactgc
    14101 ccagcgacgg cggactccgt cttagcgaac tcctccagac ttactactgc gtctgtgagg
    14161 ccacggcatc gagcagcctg ccgacagcgg cgacgcggtt cgcggcattc aagcgcgcgg
    14221 gccggcatca tctcctcgat gaaatccggc agttcgcgct cacgttcggt tacagcaagc
    14281 caatcttcca gaagctgcgc gccctgctcg tgcaaaaaaa tttcgacgag agcggggaga
    14341 aacagccgga accggtaaag cacatgaagg cgctcattca gcgcatctac gatgaattcc
    14401 tcgcgtggct ggacaaacat gctgacgcgg aagcgaagag atagcgtcgc cgaaggatgg
    14461 gtgaagaaga actgcagcaa aaacgattgg acggagtgcg ggaacgcgga gagcggatat
    14521 gacgcctgac catagcgatg gtggggccgg cggcggcgcc ggcggcagtg gggcggcaag
    14581 cggctggacc ccgccgattg tccgaccggc cgaacgcgaa gatacccccc cgcccgccgc
    14641 tgcgcccgat gacgacgctc ccgcggccga gtcggacatg tcggccgccg ccggcagccc
    14701 gccggccccc accgcgggaa aaagcgactc gcccaccgtc tccgcgaccg gactggtgac
    14761 cggaaacaaa tacgccatct gtgtccgcga ttcctatctc aatcacctgg aagccgaatt
    14821 cgatctcaaa gccggcgaca tcgacgacga gggggtgcgg cggctcaagc gccggggaaa
    14881 gaagcgcaaa cagaccaccc ccgatggatg ggtggtcgag cttcaggacc acctcaagaa
    14941 gttcgttatc ggcgacgtcg gggagagcga cggaatcttc gggaggaagg gggtgctgaa
    15001 ggcggtgcag gcgtttcagg ctgccgcgct gaccaagcac cgctggcagg ggaaagagct
    15061 caaagaggtg cagatcacct acaaggggaa gatcgacggc ctggtcgaca gggatgtgaa
    15121 agaggagatt actcgctggc tgggcaacgg ttacaccgcc tattatccaa aactcaccgc
    15181 cagcgtcggc cgccgcaaga acaagaaggg caaggtgacc gagggggcga acaggaaacc
    15241 ggatgtgctg ctcgtccagg accggctccg ggagctgggc tacgtcgcgg ccctgccgtc
    15301 ttcaatgaag attaccgacg gcgtgtgcga tgacgggacg gtgatggcaa tcaaactgtt
    15361 tcaagcgcag gcgtacgata tagacgagca cgtgaaaacc aagcggggac ggtgcgtgcc
    15421 gctcccaggc ggccttatcc agaaggggaa agaagacgag aagaggcttt tggggagcaa
    15481 tccgccccgt tacaaagagg tggcgaaggg gagcatcaag ccgattcaag ccggcatcaa
    15541 gaaacggaaa gacgaagcaa ccggcgacga gaaaaagatc tgggacaagc ttgaggaggt
    15601 ctggaatcgc gtcagtccgc atttccccga aggcacgaac atgtcctccg gataccgcag
    15661 cgatgcccgc cagcgggaga tgcttcggga aagctacaac gagacctaca aagccgactt
    15721 gatcaagaaa ttcggtaagg acgagtggga gaagtacaag aagatgcagg gagtggatga
    15781 tgttaaagcg gaaccggaaa tgcgctccat gctggcgaag gttgggttcc aaatcgccct
    15841 gccgggcacg agctctcacc gaagcggcaa ggcgatcgac gtatcggaca acgcggggaa
    15901 tgtgtatgat gcccagcaga tacgggccct cctgtgggcc cacgtgatgc tggacgagaa
    15961 gcttgtcgca aagatcctgc ccgagacagc caaaaacggc aaagttgtcc actgggagtt
    16021 tcaatgattg ttgttccctg ccttcggcga acggccgcgg ccgctgttgc ctgcctcgtt
    16081 ctcgcgctgg cgtgcggatg tgcgaaagat ggcggcaaga ctgatggaac ggctgccgcc
    16141 cgcccgaaag caggggaagg aatcctccgt cccgatgcca ctacctattt cccactggaa
    16201 gccggcagct actgggtcta cgaacggtac acggaaggcg aggaagaagg ggatacgacg
    16261 cgctatgagg tgctggcgct tgacgaaagc gactccggct gggtggcccc ggtgctcgtg
    16321 acctacagcg acggggacac cgacacgatc acctatgtct gcgggcgggg cgggctggtc
    16381 accaggaaga ccacttttga ggacgtggcg gagccgtttg cgctcctgca acccgtcgat
    16441 agtgcaaccg tgggcgattt cacttacgag caatgcccct acggcgccga ctcctgctgg
    16501 tttctcgaaa acaacccgga gcgcgatgtg gactgggtcg ggtatgagtt ccgccaggga
    16561 atcggtctgg tctccaccag cgtgctctat atgtccatcg atctcgtgga ctaccggatc
    16621 ggctccgccg gccgcgacag cctgaaagcc ggcaattcgt cgccttagtg gggttcgagg
    16681 gcgccgggtc gccgctcggt atccccgcgt cgcgacaaat tacttgacaa tgcgatactg
    16741 gaatgataag atttagttcc tgatgcaact cgcgaggggg ttcgcatcct cttgtgaggc
    16801 agacacctgc agacccatag ccgagcgacg cggcggcagt ccgcatatgg atatagcgaa
    16861 atactcatcc gattcccgcg ccgtaattaa agtggcccgg gaagttgccg ctgaattccg
    16921 acatccggaa atcagcatcg agcacctcct ggtggcggtg gtgcgccacg agggttccga
    16981 ggtcgagtcg atcctcaacc agctcaagaa aaatcccgcc acggtcgccg ccatggtcga
    17041 gagttacctc aagcagcagc cctcgcgggc cgctgcccgg gacagcctca gcgcctcgcc
    17101 ggcggtgcag gcggttctcg cgcaggcttt cgaggaaaag ggaaagctct acgacgccct
    17161 cgtcgagccc gagcatatcc tcctggcgat attcgaccgc gcctccgctc tcccgcccta
    17221 cctccgcgaa caactggagt tcaccaagga ggatttgtac caggccatgg cggagagccg
    17281 gtcggtcgag gcgatcgctg tctccccgcc ccggggcgag ggatcggctg ccccggccga
    17341 ggagcccagg acggtcgccg cggtctcccg ctactgcgcc gatctcaccg gccgggcggc
    17401 ggccggcgag tacgacccga tgatcgggcg ggccgaggaa ctgcgccagg tgattcagat
    17461 tctgctccgc cgccgcaaaa attcccccgt cctcgtgggg ggcgccgggg tcggcaaatc
    17521 ggcgatcgtc gagggcctgg cccaggcggt cgtgagcgga gcggtcccca aggccctcca
    17581 gggcgtcaaa gttctcgaag tggacatggg cgggctcgtg gccggcgcca agtacaaagg
    17641 cgaattcgaa gagcggttca aggcgctcat cggcgaggtc atcaagtcgg ccggccgcct
    17701 catcctgttc atcgacgaag tccacaccat catgggtgcg ggaagcggcg gcgccggtat
    17761 ggacgccgcc aatctcctca aacccgccct cgcccgcggc cagctccgcc tggtcggcgc
    17821 gaccaccgaa gaggagtaca ccaaacacct cgaaaaagac aaggcgctcc tgcgccggtt
    17881 cgagaaggtc aaggtcgggg aaccgccctt cgacgaggcg gtggcgatcg tcaacggcgt
    17941 ggccgcaaag tacgaggaac accaccgcgt gactttcagc cccgaggcgc gcgtggccgc
    18001 cgtcaagtac gccaaacgct acctcagcga gaaaaacctc cccgacatcg ccctcgacat
    18061 catcgatgaa gcggccagcg aattcggcgt caagaaagaa atggccgagc gcctcctgcc
    18121 ggggctggaa gccgctatga aagacattga gccgctcctg gcggccgccg acggggccgg
    18181 cctgagcgac gaggaccagc aaaaactgcg cgccctctac gagacgttcg ccgggcaact
    18241 ggacgccctc ggcgggtact gggggcaccg cctggaggtg ccggaggtga gcggctgatg
    18301 gccagacccg aactgaccct gaaagatatc cgcgaggcct tcgcccgcca gcagcaacgc
    18361 tgccgcgatc tggccgaggt ggtgcggaac ctccagccgg tcatccacga atccgacatc
    18421 ggcgcggtcg tggcccggcg gaccgggatt cccctgtcga agatgatgac ggccgagaag
    18481 gaccggctca ttaatatgga ggcgcacctg gcctccaaaa tcgtcggaca ggagaaggcc
    18541 atcacgagcg tggccaacgc gatccgcaag gcgcgggccg gactgaaact ggccaaccgc
    18601 ccggtcggat cgttcctctt cctcggcccc accggcaccg ggaagaccta cctgcccaaa
    18661 ctgctcgccg aattcctgtt cgacgacaag aacgccatgg tccggctcga catgtccgag
    18721 ttcatggaaa aacactcggt cgccaaaatg atcggcgcgc cccccggcta tgtcgggtat
    18781 gaggaaggcg gcgtgctgac cgaggcggtg cggcgcaaac ccttctcgat cgtgctcttc
    18841 gacgaggtcg aaaaggccca ccccgacgtc tttaacatcc tcctgcagct tcttgacgac
    18901 gggcggctga ccgacgggca gaaccggacg gtggatttct ccaactgcat cgtcgtcctc
    18961 acctccaact acgccgccga cctgatactc gacgccgacc gcgaagggcg ggaggtcgac
    19021 atggaccagg tccgcgaatt cctcttcacc aagttccgtc cggagtttct caaccgcctc
    19081 aatgacatca tcatctatca tacgttcacc ccggcccagg tggagaccat tgcgggactg
    19141 gaatttgccc agctcgcccg catgctcgcc gaacagaaca tcgccgtctc cctgagcccc
    19201 gaggcgcggg ccaagctcgc gcgcgacggc tacactttcg agctgggcgc ccggccgatc
    19261 cagcgcgtca tcgagcgcga catcactaac cgcctctcgg tcgagatcat caccggcacc
    19321 ctcgcgcccg gcgacgccgt cgtcatcgcc gtacaggacg atcagtacgt gttcacccga
    19381 accggggaat cccgcggata acaatactca gattcggagg atatcggaat ggccaacaac
    19441 ttttggctcg gagcccgcga acgaaaggtg aaggacgact cgatcccggt cgagctgctt
    19501 ccctcgaaca agacgctcta tgtggccaaa ctcaacaacg acgaggcggc cggcgtcgag
    19561 ccggtggagt gcaacaatct caagcaggtg ttcgaaaagt tccagccctc cttctccgct
    19621 gaggtcgaga ccctcgacgg ccagtcggcg ccggccgagt tcaaaatccg cgccatgaag
    19681 gacttccagt ccaaagaact catcgaacag aacgagtacc tgcagaaagt ctacttcagc
    19741 aaggaaatcc tcagcgatct ggacaaacag ctcaagaaga acaccgccct caagaaaacg
    19801 ctggaggatc cccagacccg ggaagccctg ctgaaagcgg ccaaatatta cgtggaattg
    19861 ttgagcgaat gagagcgagg tagaaggtaa tgggcgaaga agaaaaacgg gcggcctcct
    19921 ccggtccgca ggccgacgcg aaacccgcca aagcgaaaga gctgctctcc gacgaggagt
    19981 tcgcaggcat cctgggcgac gctttcggcg acttcaagaa gttcgccggg cttttgccgt
    20041 cgttcaagac ggccgacggc gacctggtcc gcggcgtcga gtggctcgac cccaaaaaag
    20101 aattccaacg caaagagttc ctcaccaagc cggaccacgc ccggcagcgc cggctgctcg
    20161 ccgagcggct caaactgtgg atcgaaatgc tcgccgaggg cggcagcatc gacgacctcc
    20221 gcaagaaact caacgagaaa gccgcccagc tcgagagcaa tctcgagaag aacatgaaga
    20281 aggtccacaa ggcggcccgc gacctggaaa ccggctaccg ggccatcgat aagttcttcg
    20341 ccaacgccca ggccgaaccg gacgccaagg tcaacgcctg gttcgccaac gtgagcgccg
    20401 aggaactcat ggaccccgac gacaaggaga agttcgaaca gctgagcaag gccatcgcgg
    20461 ccgagttccg cgagtggggc atcaaacgct gctactcgat gtgcgtggtt cccggctacc
    20521 tcggcagcgt ccagaacatc gacacccttg cccgccagat cggcctgccc aaccgcgtgc
    20581 acgtcgtcac cgacctgccc gatctcgagt cggtcgagga catcatggag atgctcgaag
    20641 atccgagcat ggacggcctc atgagcagcg acgagtgcaa gcagtacgtc tcgctcttcg
    20701 ccaactacct cctcgcccgc agcgccaacc agtacgagga cggcgacatg tgggtgcccc
    20761 cctcagccgc cgtggccggc aagatgtacc tcggcgatga aaccgtgggc atgcagcagc
    20821 ccgccgccgg ctacaagtac ggcaagatca acgaggccaa gtacctgcgc ttccgcgcca
    20881 accagcccga cgcctcgaag gtcaacgaga agggcgtcaa ccccctggtc agtttcgagg
    20941 gccaggcggt cgccatggga tcgtccaccc tctccaacaa ggagaccttc aacgtctact
    21001 ccatccggcg cacctacgac tacgtgtaca agacgctccg caactacctg aacaaacaaa
    21061 ccttcaacgt catcgaccag aaattcatcg acaccctgcg caaggacatc gacaagttca
    21121 tgcgcgccat ctccggcggc gacaacatcc tccaggacta caccgtgcag gtctacgccg
    21181 acgaggaaat gcgtaaacgc caggaggtgg acatcaaggt cgcgctgaac ccgaagtacc
    21241 cggcgcgaac cttcaacatc gaattcaccg cctggaacga ggataatcag acggcggtga
    21301 aggacaagta ggccgcccgg tcgtcaggag caaaaactgt cgcgaatacg gaacggtgac
    21361 tttccaacgc aaacactgga aaaggaagga ttgccatggc acatcgcccg aaactcgaaa
    21421 tcgacggggt ggagtacccc cacgtgtact ccgtgtcgta ccatgtgtac acggcgaagg
    21481 atgaaaccgg gcggcccacc gaccgcgccc acgccggcct gatcaagatc aaagtcgagt
    21541 cggcggaaga cggcaacgtg gacatcgccc gctgggcgat ggacacctcc aagcccaact
    21601 ggaaatcggg aacggtcgtc ttcttcaaca aggacggggc cgtgatgaag gaactgagct
    21661 ggaccgacgg tttcatcacg gatttccggg aaaccatccc ggacgtcaag aacaatccgg
    21721 acgagcagat ctaccaggaa ttcgagatct cgtcgaacgc gatcatgatc ggcgacgcgg
    21781 agctgcagaa ttactgggat aagtaagcgg cgcgccgcgg ccgggcggcc gtgtgccgcc
    21841 cggccgggcc ggccgacagg tcactcatga cacccgaatt tgacgccgaa ttccgggccc
    21901 gccgcgccga cgtcggggcc gacgccctcg cccgccgcgc cgaaaacgtg cccctcgact
    21961 acggcacccc tgtcgccgcc ctcctccggg tggccggcaa agagattaag gccgacatcc
    22021 actccgtccg cctcaatcag cacgtcttcg gccaccacgt catgcaggta ctcgtccggc
    22081 gcgtcggact ggccgagggg ggatccggag aattcgagca gatctccgaa tccgcaggct
    22141 tcctcggcaa accggtcacc ctcgaactgc gccccgaagg cggcgtcgtc gccggcgacc
    22201 gccggctcga attcatcggc ctgatcaccg aggtccgttt cgaccacacg atcgacggca
    22261 tcaacagcgt gctcatcacc gcccacagcc ccacgatctc gatggacctg gtgccgcgca
    22321 cccgcgtgtc gccgcccggc ggcgcctgcg ggaccatcat cggcggcatc ctcggcgaac
    22381 accccatcac ccagggccgc gtccagagct ccccggtggc cagcgccccc gccggactca
    22441 acctcgaggt cgactccggc gtccagtact ttgaaaccga ctaccgctac ctccgccgcc
    22501 tggcctcgga acaccagatg ttcgcctact acgacggccg ggcgttccac gtcgagaaag
    22561 ccgccgccaa caacgagcag gtgctcaagt ggcgcgagga actcggctcc ttcgccgtcg
    22621 gcatcggcac cggccccctg gcgttccggg gacaggtgtg ggacccccgc cggaaagaaa
    22681 tggtgaccgg gagcgccgac cggtcctcga tccgcgccgc cctgtcgccg ctgtcgcgca
    22741 cggcgctcga cggttctgag cgcgtcttca ccaccgccgg cgtgtcgctc cgcgtgaagc
    22801 accccgacca gtcgaaggtg gacggggcgc tggcgcgcga ggtcgaggcc tccgccgggc
    22861 agatgatgct ctgccgcggg acctcaatcg tccccgccgt caccgtggga agctgcgtgc
    22921 gcgtgcgggg catggacgaa ctcgacggcc agtactgggt caccgcggtg cggcacgaag
    22981 tctccgacag cggcaagtac tacaacgaat tcgagtgcac gcccctcgaa ctggcgctcc
    23041 cgcccctgca caccgaacgc caccgccgcg ggtacttcca gaccggcgtc gtcaccgaca
    23101 acaacgaccc cgacgggctc ggccgcgtcc gcgtccgcct ctcctggcag gccgaggacc
    23161 aggcctccga gttcctccgc ctgctcacct ttgacggcgg ccagggccgc ggctggttcg
    23221 gactgccgga ggtcggcgac gaggtcctcg tggcctacga gcggggcaat cccgacgccc
    23281 cggtcgtcct cgggtgtctg tacaacgggc aggatacgcc gccgaaagcg accgccgatt
    23341 gtctcgatgg cggccaggtg gcgaccaaaa ttttccgcac ccgccgcggc aacgagctgg
    23401 tgttcgacga ccgcgacggc acggagacga tcaccatcaa ccagcgcgac aacaccaaca
    23461 aaatcgtcct ggccatggat ggccccaaga tcgtgatcga gacgaccggg gatctgctca
    23521 tgaaggcggc ggccatcgcg gtcgagtcga cctccggtga catctcgatc aaatcggcgg
    23581 cggcgctcaa gggcgaagcg gccgccgaca tcgacctcag cgcccaggcc aatttcaaga
    23641 gcgaaggcgc gatgaactac gaggccaagg ggggcgtgga attcaaagcc tcggcgaccc
    23701 aggcgaccgt cgaggggagc gcaatgacca ccgtcaaagg cgggatcgtc aagatcaact
    23761 aggtatcgca tggaattgct ctcactgccc ctcctgcccg acgccggcca cttcccgaga
    23821 accgctctcg accgcagcat cgtgtattcc atcgggctga tcctgacctc ccgcctcggc
    23881 atgatgcctt ttctgcccga ctacgggtgc gacctgtggg agcgcgagtt caccgacctg
    23941 ccgacggcga acaaggcgaa catccgcgcc agcctccgca actccatcga cacccacgaa
    24001 cggcgcctct acaacgtctc ggtgtcgttc tcggaggcgc aaacgagctc cggccacgcc
    24061 ctcggcctgc tggtccgggt gaccggcaac taccgggacg gggcggagga aaaaaaactg
    24121 caggccgact ttcacgtcgg gtgaggcggc tgcgaaatgg gtgatccggc atgaccgacc
    24181 atcgcggacg ttcccaggcg cagattttcg ccgaactcta ccgccacctg cggcacttcg
    24241 accccactat gccggagtcg gccgaacggc tcgatcccat cctgcgcatc ctcctccagg
    24301 ccaacgcctt ccagctcgcc cagatccacc aacgcctcga ccagacctgg aacgaagcca
    24361 ccgcctcgct catcaaatcc atctgccccg agagcggcca ctggccggtc cccgcccata
    24421 ccatcatgcg ctgtacgccg gtcgatgatg cggtcgaagt cgatacctac acccgcttct
    24481 actacaaaga ggagcgcgag ggcggccaga cgttctactt cagcccgctc accgcgaccc
    24541 gggtgctggc cgccgccgtg gcccacatct acctggccac cgaccgcacc gccatcgagg
    24601 cggtcccggc cgggtctgcg gccggtgcgc cccgccggac gcggacgccc gccgacgccg
    24661 ccgacctccg cgccatctac atcgccctct ctcaccaagg gctgcgctcg gcgctcgacg
    24721 gagccgtagc ctttctcagc ggcccccccg aaatccaggg acagctcatg tggggcgagt
    24781 ggactcccgg ccgtctcgac ggttcgttct ccggggacgc ccgcatgtgc ccgggcgtcg
    24841 acgcccgctg gccgggcgcg gccgacagcg agagcggcga ctggggcgga ctgcgccggt
    24901 cggaagatat cttcgtcaaa caccgcaacg cgttcgtgaa aatacccgcg gccttcgcct
    24961 cggcctggga accgggaccg attgaccgcc gtctccgggc gctctgcgcc gccggcggcg
    25021 tcgccgtccc gggcgaccac gagcgcttct actggatcag gatcgacctc cccccgcgcg
    25081 gctcccgggc ggccttcgcc gaacctttcg acctctgttt cgacgccgtc ctggccgtca
    25141 acacccacga gcaggcgctc ttcaaacaca cgggcggcaa ccggctggtc gagatcgaga
    25201 ttcccgacga cctcggctcg gtcctggagg tcagcgccgt cactgattcc gccggaaaaa
    25261 cctaccgggc ctcgtatgaa atcggcgtct cgctcgaccg caacgtctac tcgctcgagg
    25321 cgcgcggcga ccggctggtg ctctggttcg atttctcaga gtcgctcgat ccgccgcccg
    25381 actccatcac cgtcgcctac acggtgaccg ccggcacggc cgccaacggg atcggaaaag
    25441 gcaagatcac caacctctac gagagccacc ccggcctgtc gcgggtggcc aatatcacgc
    25501 ccgtctccgg cgccattccc tccaagaccg aggcgcagat tctcgccgag gtctccgccc
    25561 gcctgcgcca gcgcgaccgg gccatgactt tccgcgacct cgaaacctgg gccctcacct
    25621 tcgacccccg catcaagcgg gccgagggcc gcaacagcgt cgagcgcctc aacggcggca
    25681 tctgccggtg cgttgtcgtg cgtatcgtca tcgacgcgga agatttccac agcgaggccg
    25741 aagttgacct gctgcgcagc cgcctggtca gcttcctgaa agcccgcgcc gccgtcaaca
    25801 ccatcgtcat cgtcgaggtc gtgccgcaat gaccgccgcc ggaccgcccc tcatgcccga
    25861 tctgtgcgac cggcgccaga aactccacta cgtcacggcg atcatcatcc tccacaaact
    25921 cggcgtcgac cccggcgccg tccacctgcg ggcggtggga ccgttcctga actaccgcgg
    25981 cgaggtgcac tcgcaggagc ccgctcccgg aacgcccctg caccccgaca ccgagatcag
    26041 cctcgacgtc gggtactcca gcgtggtcga ctatctccct taccagtttt tccacggcct
    26101 ggagggtgtc agccgcctga gcggcgtgca ctgggaagac cgcgcccgcg ccctcatggc
    26161 gcccttcgac ggttccgtca tccgctggac cgccgaggct cgccacgagg tgatccggtt
    26221 ttccggcggc gccaccgacc gccgccacgt tctccgcgtg ctcgacctct tcggtttcag
    26281 cctggaaacg gccgccatga ccacctccga ggccctcttc tggctgaccg tcatgcccac
    26341 tttcaacaac tggtcgggaa accccgcgcg ggtcgcgcgc atcctccagg cggtgttcgg
    26401 cctcccgttc tccatcgctg agaacgtccc cctcccgcag gagatccccc aggacattca
    26461 gacccgcctg ggacagcagt tcagccggct gggaaacgag ctggttctcg gccgggagta
    26521 tacggaccgc gacaacgggt gcgaggtggt cgtgcacgag gtccccgccg accgcgccgc
    26581 ggactggatc gagggaggcg cctgccggca gcgcctggcg aacatcctcg agtttagcat
    26641 gcccggacac atggactgcc gcatcaggat cgaggtgcgg gaagaacccg ccccgctggg
    26701 ggaaggcccc gccggcgcct acctcggggc ctcgctgcgg ctccaccgcg agccgggggc
    26761 ggccgccgta cgtccggtca atgaagttga ctccacgggg agcaggttgt agattcggat
    26821 gaaggaatcg gggaagaatg gatgagtaat ttccgcttgt actcggtcaa ctggcaggac
    26881 ggcatgctcg tcgcccgcaa acacctcctc gacgaggagc gctacgtcga ggatctggcc
    26941 cgctggtacg cctatccggt gggggagcgc tacgggctgg cgcgcaagtc gtctgacggg
    27001 cggccggcgc tcggcctgac cgtgctcgtg cacggcgccc gggtgcgcgt cgaactcagc
    27061 cgctgccagg ccatcacctc cgacgggacg gtcatcgaga tcaacgaatc cggccccgag
    27121 gtgctctttg ctgaggcgcc cttgcagggg gaccggatcg acgtgttcct cgcggtcaat
    27181 cgcgaggcca agcggccggt cggggcgccc gatgcccgtg agctggtccc gcgcgcgccc
    27241 ttcgccgccc gcgactacgc cctccacctc ggcgaaccgc ccgcgctgcc ggaatctgat
    27301 ttcctccaga tcgcccggct caagatcgcc ggcggcgacg tcgtccaggc gcccgactac
    27361 tatcccgcgt gcctgtcgat gtacgcgacc gacggcgcct acgagaaaca caaggagtac
    27421 tgcaaccgcc tggagagcct catggcggtc atctcgcgcg cccacctggt ggccgcgacc
    27481 gcccccccgg ccggcggccg caacgagctc ccggcgggga tcgaggcgct ctgcgatcgc
    27541 gtcggcgcgc aactggcggc caccttcgac ctcctcgccc tcggacccgc cgcgccccac
    27601 cctcacgagg tcgtggtctt cttcaagcgc ctcttccgca ctgtcgccac cgcgctcgcc
    27661 taccgcccgg cggtcaagga ctacctcaac gaccggctct tctcccgcac cctcggctcg
    27721 agcatgggcg ccttcgtctc ccagatcgac agcttcctcc tcaccgaata caaccaccgc
    27781 ggtctctcgg agcattttca cgccatcgac gccgtcctcc gcctgctcca ggagaccttc
    27841 tccttcctcg cccagagcga ccggatcgcc cccgaaccgt cgatcaccga gaccgtcgag
    27901 taccagggac gcacgtatgc gatggcggcg acttcgggcc tgcgctgcga ggagcgcgac
    27961 ggtctgtgct acctgctggt ctccctgccg gcggccatct ccctctccga cctcgtcgtg
    28021 ctgatggcca aggagctgtt cgacgtgagc cagtggaacg ccatgcaggt gcgcctggga
    28081 atgaatgacg cgctcggcct cggccagacc gaccccgtga aagtcgacac ggtcacctac
    28141 ggcagtaagg tcgtgctccg ggcgcaggac atggtgcgca acgccgccgt caaacaggtc
    28201 aacctgatct tcaggggaaa aggcgacgtc cggaaattcc gggaactgaa accgtccgaa
    28261 ctcctgatct acacggccga ttaacgcccg aaccggggct gtgctatgga aataaagcag
    28321 tacatcgacg acctcttccg ctacctcgcc acctacgaga cgagttatac gtcgtttcag
    28381 acggaggcgt tcctgcagac gtacaacggg attcgggcgg tcttcaccgc cctgcggcag
    28441 cagcgcaacc aggccgtgga gaccgactac accttcctcg atttcatcca gagctcgccg
    28501 ccggcgggat ccgacttgcg cacggtgacc gtgcagctgc tcattacgtt tttcgagtcg
    28561 gaggccgatg tcgacggccg gtcgaatcaa gcttacacct atgtgcgcgg gctgcgggcg
    28621 atcaagcagg atgcgcccta cctggaagat catttgctgc cgatcctgtt ccgtccgacc
    28681 gccctccgga ctaatttcgc gctcaaccgg tttctgcttg aggagatggc gcggttcctc
    28741 aattcctacg gcccgccgct cgacatcggc cgaggcgtcg agagcttcag cgccctgcgg
    28801 gacgaccgca agattctcga gttgtgtcgg cggcgcctcc gcttgggcgc gaacctggtg
    28861 gaggatcgcg ccagcctgga gttcagcctc cggaacgccg gcgccttcga ccagctcaaa
    28921 aagcagggac cgctctacga gcagtacctc aaggagtggg gactgctgcg cgagcgcgag
    28981 cgcttctggt cccgcgtcgg ccggtcgctc gccgccacgg ggcgcaaagc caaggggctc
    29041 ttccagagct cccggtattc gagcctgatg ttcacccagc ggcgaccggc ggtcgcgctc
    29101 cagatcggcc tggtgctcgt gtggatcgcc tgcgccatcc tcgccccggt cctctggggc
    29161 aagtactcgg attcccggta ccgggatctc gatcgccggg tgaacgaact aaggcagcag
    29221 gtaacaccct gagaacggga ccgccgcggt cccgatcaag gaaaggcttt cgtggatttc
    29281 gcaaaagtgc tcagtctggg aaagaaggcg ggcaagggct cgaagaaaaa gggcggcgcg
    29341 ggccgcaccg ataccggctt ctcctggccc gcgggcgtcc gcatcggcgt ctacgggcac
    29401 tggaactcgg ggaagaccgt ctatttcacg gtcctcaacg aggactgcaa gttcgcccgc
    29461 gacctccaga tcgcggtgac cgacaacgcc accgctgcgg ctttcctggc ccaccgcgaa
    29521 gcgatctggg gcgtggccgc ggctgccggc gaaggtaccc ggatcgacac ccgccgcgac
    29581 cgcaaattcc ccgactccac caagaccgac cgcgtcctcc agttcaccgc catcctcggc
    29641 ggcggcgacc gcattcccat cgtgagctac gattaccccg gcgacgccgt cgccatcggc
    29701 acctccagcg agttcgcccc caaagtgcgc aacttcacag ccgggtgcga cggcctcctg
    29761 ttcttcttcg acccgaaagc gctcccctcc gaatcggact ggcagtccca cgcggcctcg
    29821 ttcgtcaaca tgctccgcga gctgggaccg ctggaccgca acctgtccat tcccatcggg
    29881 ttggtggtga ccaaggcgga cagtctcccc ggttttcgcg gggaggcgca ggtgagcctc
    29941 gtgccgcacg ggcaggagcg caccatctgc cgcgagtacg aggaattcct ggtggaggcg
    30001 ctggccttcc ccgaagtggc gggcaaccag gaatgggccg ggtcagtccg cgaaatcctc
    30061 gtcaaaacca aggagttcat ccgggtcgtc ctcggccgga cgctcgactt ccagatcttc
    30121 ttcgtctcga gcaccggctc tcagcccgag aagatcgggg ccgagaaggg gcggagcctc
    30181 tacaggccgc cggagaaaat gagcccggtg ggactgcgcg agccgatccg gtggctcctg
    30241 caggcggttg tgcgcaaccg gcggctggcc cgcctgcgca ccgtcaaacg ctatgtccgc
    30301 aacgccgccc tgctgtggat tctcgtctgc agcctcgcct tcggctggca cctctggtgg
    30361 cagctcgggc ggacgaccgg cctggaggac agcatccgcc acggccggac ggccgccgcc
    30421 accagcaccg aagagcgcaa caagatcggc accgaataca acgggtatgc aggacggtgg
    30481 ctggtggcca aggttttcag caagttccag gcgcccgccc gagaaatggc caagctctat
    30541 cgcagcctga agaccgaaag cgaaacctcc gagttcgacg aggtaatcca gcggcttgcc
    30601 ggcgtcatca agaacgagac cgcctggccc gcgtacgatg tcgtggcctc ccggttgcag
    30661 ccgtcccccg cctatgtcga gctgcgcgag gaggtcgccc ggtatgcggc ggactccgcc
    30721 gcgcccgtcg gccgccgggc cgcccgcgtg atgaacgctt ggagcaagtt tgactcatgc
    30781 caggtcgaca ggtcggattc acagcgctgg agtaatctcc aggccaccgt gaacagctat
    30841 caggccaact acaagccgga aatgaacccg gccgaaatcc agttgtactc ggctttcacc
    30901 gcggccgccg cggcccatgc ggaaaaagtg gaagttcagg cccaggtgga gcaggccggc
    30961 gatcagttcg ccgacatcat caaacgcatc aacggcgacc ccaccccggc cttccgcctt
    31021 gacgccggtg cccaggagct ccgtgactac ctcgtgcagc tccagatgga tccgcagaaa
    31081 tacggcaagg aaatcaaggc cgtaacggac tatctcagcg aggtcaagaa gtgggagtcg
    31141 cgccagactt tcaactatcg cgtcgacatc gtgcccgatg gcacccacct gcacatgcag
    31201 gtagtgggcg acggtgagga accgaactgg tcggacgcca acaaccaact cttcgccgat
    31261 gaggcgggca acgttatgtg gaaagtgggg gatgttgtct atgttgttct tcacaaggcg
    31321 cacgtcggcc aggacgagtc gtggggaagg agctttgata gccgggtgat tctccgcgac
    31381 ccctacgcgc tgttcgaact cgaaaaggaa atcaagttcg ggcagttcgg ctccgccaaa
    31441 atctcgttcg tccccaagag cctgtccgag agactgcccg tgttgaagaa gtgagcgaat
    31501 gtgggatatc ggaacgatag ttgccggagg aggatctgat atgtgtcaac ggacttgtcg
    31561 ccagtggatc ggcgccgccg ccgcggcgtt cgctgtagtg cttctcgcgg tcccgagtta
    31621 tgccgggaag gtcgagctgc ccaagggcag caaactggtc gttgcgtttg agggaaacgt
    31681 gtcgtccagc gtgtttgcgg tcggcgacag cctccccatc gttcttcagg aggacgttac
    31741 ggtcggcggc ctcgtcctcg tggagaaggg cgcgcggggc acggcggtgg tcgcgaaggc
    31801 cgaaaaatcc ggccgggcgg gcaaacccgg catgatcgcg gtgatattcg tcgacctgaa
    31861 acccaagggg cagtacccgc tcctcgatgt gcccgccatc gcattggagg gtggcaccaa
    31921 cgagcttaag gggaagggga agaaggtcct ttcgtacctg ttcattttcg gcctgttcat
    31981 caagggcggc gagggcaagg tgccggcggg cagtacgttt gaggccaccg tcgccgaaag
    32041 cgtcaagttc gaagtgcagt agcgcgcttg cagcccccgc cggggccgcg ccgccgcgac
    32101 ggccgcggga tcggccgcgc gactcggggc cgcagtttcc ggcccgccat ccggggcgaa
    32161 agcgggcgcc ccacaggtct ggctcatgac tcttatcgaa gaagagagaa tcttccgcga
    32221 tctcctgcgc ggctgcgacg gctttaccgg atccatcttc cggttcatcc tgcgcctcat
    32281 ccacgtgcac gtgctcgaag tggaggacgt gctgttccat ctcaacagcg cggtcatgat
    32341 gccggcgcgg cccgccggcc taacgtccgc cgacgggacc gaggacgacg tctccgattc
    32401 cgatgacgcg cgcgttcagc agcagcagga cgcagtctcg ggcctcgacg tcatcggcgt
    32461 cgccctccgc cagttcgagt tcgacccggc caagcgtctc ctcatcgccg ggcacaccga
    32521 cacctccggg gagccggcca tgaatttcgc gctctccgat ctccgcgccc ggaatgtgtt
    32581 cgcgctgctg actggcaatc gcgccgagtg ggcgcggatc tcggccgacc ggcaccggat
    32641 tgaggactac cagcagatcc tcacctggat cagcggggag ccgcgctggg gatggccctg
    32701 cgacccgcag ggaatcgaca acacctgggg cacccacacc cgcgacgcca ccgagcagtt
    32761 cgtccagcgc tacaacgccg ccttcgcatc gtccccggcc gatctcccgg ccgacggatc
    32821 tccgcccccg cccgactacc gggcgccgct tccggcgaac atgcaccggc agatcgacgg
    32881 ggccgccggc caccgctggc cggagccggg ctgggccgcc ttcttcgacc tgtaccagga
    32941 ccacctggcc caccgtctgg gcctctctgt ccccgacctc gtcagcctgc agaccaaccg
    33001 gccccgcttt atttccgagg atgtgaaatt tgtcggctgc ggcgagtcct tccccgtcga
    33061 cgatgccgag cgcagcaact accgctccca gctcaaccgc cgcgtggaac tgctcttttt
    33121 cgacgcccac gaggtgccga acctggacgc gccgaccatg gcctgcccgg ccgaccggac
    33181 acgcgtgcac gagcgggccg agtgcccgct gtacaacggt tgccactacc tcccggtcta
    33241 catcagcccc gaggacctca ccgcggttgc ctaccacctc cagttcatgt actgggaccg
    33301 catcagcggc agccgggagt gcgtcccgga gggactggag atccaggcgt tcgaggacgg
    33361 cgacgcgccg gtagccgcgc gcgtgcagta ctccggcggg acctacaccg tgaaagtgcc
    33421 gcacaacccc gcgcgcacga agctgtactt ccagtttgcc gcccccggca agtggatctt
    33481 cacggccgat gcgtcgtccg cctccccgcg gctggtcgat ctcaccccgg agcagtacgc
    33541 cgcgctgccg gcccacgagc gcgcccatta ctatgacctg ccggcggtct ggtcctcgcg
    33601 caactactgg acgcgcggcg ccggcgccgc ctacaccggc ggcaagcggt tcgaggaggt
    33661 catggccgcc gaccgccaac tcaaacccta cggcgccaac accaccacct tcgagcagcc
    33721 gctcgtcttt tctctggacg atattgtgct gctcgatagc gtcggcggca gccaggatat
    33781 caaggacgcc aaccaccggg gcgccgagcg caatctcgcc gccaagtcgc gggtcaagct
    33841 cctggtggtc gacccggcca cgggattcct caagctgtac cagccgggcg ccgacctgaa
    33901 ctccgcccgg atcccgttcg cccggaatct catcaccgag cccgccgaag acgtcaaaat
    33961 cgttttcttc cgcgacgggt tctatcccgt gggccgccgg cgcaccgagc cctcgccgaa
    34021 ctggccggcc aacggattcg tggtcggcgc gcgcgccgcc gtgcgcaacg acagcgacta
    34081 ccactgcacc tgggcgcttc aggaaacccg caccgaattc gcctccaccg gcgatttcga
    34141 cctgcactac ttccacgcac ttcatgtgga cgacgagcac ccgatctcgt tcaccatctc
    34201 gtatgcgtcg atgagcttca tggccgatac ccgcgccccg gctcagttcg gaccgatccc
    34261 caccgaggcc gaggtcgaca acttccgcaa ccagggatgc tatcgggcca tggatgtgtg
    34321 gaaccggaaa cagctcttca tggaggaaat cgcgccttcc gcgcgcacgg tcattatccg
    34381 gcatttctat ttcttcgacg agcgggagca tttcaagccg acagcgccgg cgggcgggtg
    34441 ggtgaactgc aatttcgaca accaaccgtc ggataacgcc gccgacaact tcgagaccct
    34501 cttcgcccag caggcgctgc gcgacgccga gcgcgatgcc ctgggcggca aggcgaaatt
    34561 cctcgccctc gtctgccgca acgaaaacgg cgactggggc ccggcctacc aatgggccat
    34621 tcgcgactcc gccaaccgcc cctactcgct gctcaaactg aacaagtcct gctaccagga
    34681 tacgcccacc atcctgaacg gcatcagcgt caccgagcac ggcgacacct gggtgccgaa
    34741 tacgatggcg cacgaactgg gccacggcac gggccagccc gatgagtaca tccgccgcca
    34801 gttcacgccg cattcgggcg cgaacgccgg cgccaacgcc aatttcattc agcactacat
    34861 cccctactcc atgccgttca acaacggctc gctcatgtac tggaactgcg ccccccggtt
    34921 gcggtacctc tggtacccga tgcgcaagat caacgtggag agccgccggg tgggatcgcc
    34981 gctgcgcgcc ctgcttccgg acaagacctt cgtggcccgc ctcgaccgcc cctccttcgt
    35041 tttcaccttc caccgcaaag tcaccggcgg caacccctcc gtcccggccg tcaaagagga
    35101 cccgatcgca agcgagaagc aatacgagct cacctcgagc cccgtgcgca agctcagcct
    35161 tgagctgtac gacgtcggcc gggacgagtc gtcccgcgac accttccacg ccgcccaggg
    35221 cggccgcgtc taccacgccg tgctggtcgt gcgcgtgctc atgaaaatcg agtggaccgg
    35281 atcgtggacc gacaaccgca agcgcgaccg catcgtcgaa atcgaagatg ccttcaaggc
    35341 ctggggcgga cactaccggc tggagggcgg caccggcgac atccgcgaca tctacatcca
    35401 cttcctgccc ggcttctatg cctccagcga cggcgacacc gagtccgaca agcattacga
    35461 cgtcgagctg acgcgggggg ccgagaccgc catcagcacc ggcttctggg acagttccga
    35521 tctcgaggta agccgcgtca tcaccggcgc ccagctcgtc gattactttt tcaaccgcgc
    35581 ctccggccag accggcgagc aggcgctcca cttcctcgcg gattgggccg atggccgcct
    35641 tccgggaacg ttcacgctgc acagttgggg cggcccgccg taagggggac cggccgcaaa
    35701 ggcgaaacca atgataccgc gtcgtcaaca tccagccgcc ttcgaaagga atgggaacta
    35761 tggtcaaatg gccgaccctc atcggcgcct tcctcgcgct ggtcgtgtgc gcgcaaccgg
    35821 ctcatcagga gaacaccgtg accgataacc tcatcctcaa tccccgcgag tggggcgacc
    35881 gccagagcac cggctatctg ccggtgtccg gacggctcgg acaggagacc gtgtggcaga
    35941 aggcggccga tgcggccggc gactccgtgc tcccgcacca tctctgcctc atcggcgacc
    36001 gcatcgccgt gtggtacggc cgccgcctcg acctgcgcag cgcggccacc ggcgaacccc
    36061 tctggtcgca ggagacgctc gaaaactgct cgttcgaagc gcagagagac ggccttgttg
    36121 ccttcgatga acacggtgcc ttccggctgc tccgctatga cggcacttac ggcgagcgcg
    36181 tccgggcgcc gctgtacaac ggcgccggac gcgtctttgt cgctgatgcc tccgccgacg
    36241 ccgtcttcct ctgcgcccag atgcaccccg gaccgacgag tacggcgggg gagaaggtcg
    36301 tcggcccgga gatcatcctc gccgccctca accggggcgc caacgagctg gcctggtcgg
    36361 tgatccggga cggttttctc atcgcggccc tgatcagtcc ggacggccgc caggcttgcc
    36421 tcgcgacccg ggaccggctg ctcctcgccg ccctcccggc ggcggccgcc tacgatccgg
    36481 ccgccgaact cgaggttgcg gtgatcgaga tcccgttcga cagcgtgatc gcctgcgcct
    36541 attcgcacgc cggcgagatc ctggcggtgg ccgaaaaatc cgacaacccc gagggccgac
    36601 gcgagcgtgc cctctttgtc ctgtcgtccg ccggcgagga gcgccgccgc ctcccgctgg
    36661 ccggggcggg aagtctcgcg cagccgccgg cgagttttcc cgacggcgac gtctgcctct
    36721 cgctcggccg cgaaatctgg cgcctccgcg gcggcgagaa gctctgggtg cagaaaatcc
    36781 ctgccgagcc gggacagatc ctcatcacgc tgctgaaaga cggctccctc ctcgccgccg
    36841 ccggaaaact gctgctgcaa ctgtccccgg agggcgagga aatcagccgc cggattgtcg
    36901 acgggccgat cacctgccgg cctgtcatgg atccgcaggg ccgcgtctat gtcgccggcc
    36961 ccggcggcgt ctactgcctc cgctaggatc tcatctgccg gggccgtctg cgcgcatcgg
    37021 gccgcgcgaa cccgccgcct gcgcgaaagc gaagccatgg tgaattcata cgggaatgtt
    37081 cttcaggcga tcggcgacac gtcgatggtg cagctccgca gagtcgcgcc gccgggcgcc
    37141 gccgccattg tcgccaagct cgagtgggaa aaccctaccg ggagcatgaa agaccgcgcc
    37201 gctctggcca tgatcgcccg ggccgaggcc gacggccggc tgaaatccgg cgacacggtt
    37261 gtcgagtaca ccggcggaag caccggcatc tcgctggccc tcgtctgtct cgcccggggg
    37321 taccgcctgc gcatcgtctc ctccgatgct ttcagccggg acaagctcag gcacatggcg
    37381 gccctgggcg cggagctgac cctggtcccc agcgagggcg gcctgaccac caagaagctc
    37441 attctcgata tgatcgaggc cgcccgcgag ctcagccggc aaccccgcac ctactggacc
    37501 aaccagctca gcaacgccga cagcatcgag ggctaccggt ccctcggcga ggagatctgg
    37561 acccagaccg gcggccgggt cgatgccttc gtgcactgcg tcggcacggc cgcgtcgctg
    37621 cgcggtgtcg ccgccgtcct gaaagagcgc cgacccgcca tccgcgtggc ggccgtcgag
    37681 ccggcggaat ccgccgtcct ctccgggggc cgcccgggac cgcacaagat cgagggcgtg
    37741 gggatcggct acaccccgcc cctgtgggat cccggcctgg tcgatgagat tgtcgcggtg
    37801 ggcaccgccg aggccaaggc gatggcgcgg cggctcgcgc gcgaggaggg gatctttgcg
    37861 ggaacttcgt cgggggcgaa cgtcctcgcg gccatcgcgg tcgcccggcg gctcggcccc
    37921 cgggccacgg tcgtcaccct gatggcggac acggggctga aatacgtgag caccgacgta
    37981 ttcgagggat aggggagggt ccgccgccgg ctacagcgcc gcgcttagcg cggcgccatc
    38041 aaaaactcgt gcatggagcg ggcggccttg cgaccggctc ccatcgcgag aatcaccgtc
    38101 gccgcacccg tcaccacgtc gccgcccgcc cacacccgcg ccagtgaggt gcgcccggtc
    38161 gcgtcatcgg ccacgatgtt ccccttcttg ccgatctcca gccccgccgt cgtcgtcggt
    38221 atcagcgggt tcgggctgtt tccgatcgcg cacacgaccg cgtcgacggc cacccggaaa
    38281 ttcgaatcct tgaccggcac cggcgagcgg cgcccggagg cgtccggctc gccaagttcc
    38341 atgcggatgc actcgatctc ccgcacccag ccgtcgctgt cgccgatgta cttcaccggc
    38401 agcgtcagca aatccagaat caccccctcc tcctccgcgt tctcgacctc ctcgagccgc
    38461 gccggcaact cctggcggga gcggcggtag atgatgcgcg tctccgcgcc caggcggagg
    38521 gcggtgcggg cgcagtccat ggcgacgttg ccgccgccga tcaccgccac ccgcgtgtgc
    38581 cccttgatcg gcgtcgggga gtcggggaag aggtacccct tcatcagatt catccgcgtg
    38641 aggtactcgt tggcggagta gacgccgttg aggttctcgc cgggtatctg cataaaccag
    38701 gggagtccgg cgcccgcccc gatgaagatc gcgtcgaatt cctcaagcag gcgggcgagc
    38761 ggccgcgtct tgcccacgac gaaatcggtg atgatctcca cgcccagcat gcgcaggtag
    38821 ttcacctcgc gcgcgacgat cgctttgggc agccggaact cggggatccc gtacatcagg
    38881 acccccccca tcttgtggag cgcctcgaag atcgtgaccc ggtggcccag cttgatcagg
    38941 tcgcccgcga ctgtcaggcc cgccggccct ccgccgacaa ccgcgatcgt gcgcccggtc
    39001 ggcgcgggga tcgacgggag ctcgggatcc ccctgggccg cctcccagtc ggcgaggaac
    39061 cgctcgagcc gcccgatcgc caccgcgcag cccttcttct ccagcaggca ggtcttctcg
    39121 cactgctctt cctgcgggca gacgcggccg cacacggccg ggagacagtt cttctccttg
    39181 aggacccgcg tgccgccggc gaaatcgccg tcggcaatgt gctggatgaa ccccgggatg
    39241 tcgatctcga ccgggcagtt gctcacgcac ttcggcttct tgcactgcag gcagcgcttc
    39301 gcctcggcca cggccgtcgc gatcgggtag cccagcgcca cctcgtgaaa gttgcgccgg
    39361 cgcacctcgg ccgcctgctt gggcatctcc cgccggtgcg ggtcgatctt ctttcgctgc
    39421 ggttcttctg tcatggcgcc tcgtccctcg gttacgcgtt gccggcgtgc tcctcgcacc
    39481 ggctcgtcga ctgcggaata atctcttcgg tcaaatacga cttgcggcgc tggagaagct
    39541 cagtccagtc gacctcgtgg ccgtcaaagt ccggaccgtc gacacaggcg aagcgggtgg
    39601 cgccgccgac cgagacccgg cagacgccgc acatgcccgt gccgtcgatc atgatcgggt
    39661 tcaggctgac gatcgtcttg atgccgaggg ggcgggtcat gtccgacacc cgcttcatca
    39721 ggaacgtgca gccgttgacg atggcgcgat ttatcgggcc cgactcctgc ccgatcacct
    39781 ccgacaggcg cggcacgtgc ccgcgcagac cgcgcgtccc gtcccgcgtg atccagacca
    39841 cccggtgcgc cgccgcccgc agcttgtcgt cccagaacgt gaggtaggag ctgcgggcct
    39901 cgccgacgac gatgacgcgg ttgccccgct cgcgcagcgc ccgcgcgatc gggtaaatgc
    39961 tcccgatccc gtagcatccc ccgacgcaca gcaccgtgcc gtaggtgtcg atctccgtct
    40021 ccttcccgag cggaccgacg accgtgagaa ggctttcccc tggccggacg ttggagaggc
    40081 ggttggtggt ggcgccgacg ttcttgtaga cgacgacgat cgtgccgtcg cgggcgtccc
    40141 agtcggatat cgacagcgga atgcgctcgc cgtcatcctc ggcccggacg atcacgaact
    40201 gtcccggccg cgcctcgcgc gacacgtggg gcgcctccac ccggagcatg tggatgttcg
    40261 ggacgaccat gcggttgtcg attactttat acatggcatt tcctcacctg cacgcggcgc
    40321 gggttcgcac cgtcgagttc gcccggcggc agcaccacgt ggagcagacc ctcgggctgc
    40381 gccgccgtca cgcacaccgg ccagcaccgg tccagcccct ctgcggtaac cgccaccgtc
    40441 tcgccgtcgg caatccccat cgtccgggcg tcgaccgggt tcatctcgac cacccgcagc
    40501 ggatagagcc gcgacagacc ggccacccgg tcggccatgg cgaacccccg gtgggtgtgc
    40561 tcgaggttgg tggttaatag cagaaacgga tttgtcgccg agccgtccga ccggggtgcg
    40621 gacgcggccg cgggcaagtc gcactcggcc agcggccacc ggtgcggcgc ccgcgcgggt
    40681 gcatcgaatg cgtcgaaacc ttcgatcaga gtcgcgatct cctcgtggat ctcccgcgcc
    40741 gagccgaacg cgaaccccgc cgcacccatg gcgcgcgcga tccggcagac aatctcccag
    40801 tcgggcgccg cgtcccccgg cgggtcgagc gcccggtgca gccgctggac gcgcccctcc
    40861 ccgttgatca ccgtgccgtc ggtctcgctg aacgccgccg ccggcagcac gaggtcggcc
    40921 ccggtgaggt ccggcgggtg gaagatgttc tggaatatca ccgtctcggc gccggcccgc
    40981 ggattcggga tcacctcccc cagaaggtag aggaccttcc ggcgcgggcc gcggccgacc
    41041 agggaactgt tccactgcgc gccttccgcc ggcagcgcgg actgccagcg ccgggcgagg
    41101 cggtcccggg cggcggtgtc ggcccaggct gccccgccgg ggagcagctc gccgaccgcg
    41161 cccatgagca gggcgccgag gaagttgttc tgggagggaa gcggcatcac cgcggccccg
    41221 agcgccgccg cccaggcgtg caccgcccgc agcaggtcgg gcccgtccgt ccggtccaac
    41281 agcatcggcc cgagcaggat caccgtccgc cccgactccc tgatcgtccc cgcggcggcg
    41341 gcgatatccg ctccgcccgt ggtctttccc cgcccgccgc gcgcggccgg agccgtcgcg
    41401 gccatcaact cccggagcag ctccccctcc tggccggcgg ccggccggag ccagtgagcg
    41461 gcgatctgcg ccagatcgtg cggccggtgg tgaatcgtga caatgcgcgc gccccgccgg
    41521 aacgccttgc gcaactcggc cccgatcacc gaccggccga aacgcgtatc cagcccgatc
    41581 gcgatcacgg cctccgcccg gcgcaaatcc tccagcgtcg ccggcgcccg caggtgctcg
    41641 acgtacggcg cgcaactcgc cccgtaggcc aggcggaacg tcgtgtcaat gttgttcgaa
    41701 cgcatgaccg cgcgtgcgaa tttctgcgcc acgtagagat cctcgttgct gcagttcgcc
    41761 gaaacgagca ggccgaactc ctccggtggg cacgcggtca gccgctcggc cgccagagtc
    41821 gccgcctcct cccagccgat gtcgaccagt accccgtcgc gcagcttgta cgcgtgccgg
    41881 agccgccgcg gatgggacac aatctcgggg atacagaacc gccccttcac acatagctgc
    41941 ccccgattga ccaggtcgtc ctcggccggc agcgccccga ccacttcgtc tttcttgacc
    42001 tgcaggcgca cctggcagcc gacaccgcaa aacgggcacg tcgtggtgac ctcgcgctcg
    42061 gcggcccctt tccatttgcg cgctttctcc gccagcgcgc cggtcgggca caccgacacg
    42121 cacgcgccgc agaactcgca gccggcctcg agatgcgtcc ggtcgaacgc cggaccgatc
    42181 accgtttgcg gcccccgccg cagaaaggtc agtgtccccg ccgtccggat ctcctggcac
    42241 atccgcacgc accgcccgca gagaatgcag aggttgtagt cgcggtcgaa aaacggatcg
    42301 tccttgtgca ccggtaggtt gcggtagtag acggggtagc cgatctcggt gatgccgagc
    42361 cgggccgcca cctcctgcag ttcgcactgg ccgtcgttcg ggcagtagcg gcatccggtg
    42421 gtcacgccgg ccttccgaat cgtggccgag tactgccggc attcgtcctg ctcgtcgcac
    42481 accaggcagc tgcacggatg ctcgctcagg atcaggcgga gaatctcggt gcggatctcg
    42541 tgcagttggg cggtgtgggt gcggatcacc atgcccgatt cggccggcgt cgtgcaggcg
    42601 gtcggaaaac cgcgcatccc gtcaacctcg acaatgcaca tccggcaccc gccgtagggc
    42661 gtgaggtcct tgtgggcgca cagggtcggg atgtagaggc cggccgcgcg cgcgatctcc
    42721 agaatggtcg ccccgcgcgc cgcgctcagg gaacggctgt cgatggtgac ggtgacgggg
    42781 tcatggggtg agttcattgg gcacactccg tttggaccgg atgatgatgg cgtcgccggc
    42841 gatggcgtcg aaacggcaga tctcgtaaca cgcccggcac ttgatgcact tgtcgggatc
    42901 gaggttgtgc gtctccgagc gcggacccgt gatcgccccc gtcggacaga cgctcacgca
    42961 gcgctggcac cccgtgcatt tctcgggcac cacccggtac tcgatcagct cgcggcacac
    43021 cgaggccgga cagtgcttct ccgtcacatg caggagaaac tccggccgga aatagcgcag
    43081 cgtcgtcagc gccgggttgg gcgccgtctg gccgagcccg cacagcgagc cccgcttgac
    43141 cgtctctgcc agcacctgca gccggatcag gtcctccggc gccgcctggc cgagcgtgat
    43201 gcgctccaga atctccacca tgtggcgcga cccgacccgg cagggcgtgc acttgccgca
    43261 cgactccttc tgcgtgaaat cgaggaagta ccgggcgatg tccaccacgc acgtgtcctc
    43321 atccatgacg atcagccccc ccgaacccat gatcgacccg gccgccgtca gcgactcgta
    43381 atccaccggc gtgtcgagga actcctcgga gaggcagccg cccgacggtc cgccggtctg
    43441 gaccgctttg aacttcttcc gcgtgccccc gccgatgtcg aaaatgatct gccggagcgt
    43501 catcccgagc ggcacctcga tcagccccgg ccgccgcacc ttgcccacca gcgaaaacgt
    43561 cttggtgccg tagttcccct gcgtgccgta ctggcggtac caggcggccc cgtgccgcag
    43621 aatgtgcgga agggtgccga gcgtctcgac attgttgatg atcgtcggct tcccgtggag
    43681 gccggcgatg gccgggaagg gcgggcgcga gcggggcatc ccccgccggc cctcaatcga
    43741 tgcgatcagg gcggtctcct cgccgcacac gaaagccccc gccccctcct tgatcgtgat
    43801 gtcgaaactg aaggccgagc cctggatgtt ccgcccgagc agccccagcg cgcgcatctg
    43861 ctcgatcgcc cggcggagcc gcacgatcgc caggggatac tcggcgcgga tgtagatgta
    43921 cccgcgggtg gccccgatcg cgtaggcggc gatcagcatc ccctcgagca ccgcgtgcgg
    43981 gtccccctcg atgagcgagc ggttcatgaa cgcccccgga tccccctcgt cggcgttgca
    44041 gatcaggtac ttttccgagc ccgcctgcga ccggcacact tcccactttc gatgggtcgg
    44101 gaaaccggcc ccgccgcggc cgcggaggcc tgcctcgcgg acctcggcga tgaccgcctc
    44161 cggcggcctg cccagggccg cgaggaatcc cgcgtagccc tcgcgcgcga gatagtggtc
    44221 gacctcgtcg gggtcgatga tgccgcagtt gcgcagcacg atccgcacct gcgctctgag
    44281 catcggcagg tcgaaaaacc gtggggtctc cggcagctcc ggcagcggcg cctcgccgaa
    44341 atgccccagc gcctgcttgg gaagccagtt gtctttgacc aggcagctct cgatcagatt
    44401 gtggacgcgg ccgggaacga cgttgccgta actcacccgc ggccgccccg gcgcggtgat
    44461 gtcgaccagc ggctcaaggt aacagggacc gatgcagccc acctgcatga cgcgcgcctt
    44521 caggcgcagc tcccggagcg tgtcttccac ggcggccagc acgtcgagcg ccccggccgc
    44581 ccgcccgcac gaggccgtgc cgatcgttat caccggaacc cggctggccg ccagttcctc
    44641 ccagacgcgc cgcgcctcga cgacccgttc ggtgaaatca ctcatacttc gcaagcacct
    44701 ctttcaggcg gatgaccgcc atccggctgt gaatgtcgtc gtcgatttgc acgaccgggg
    44761 ccagcgcgca gcagcccagg caggcgacgc gctggaaatc gaagcgccgg tcgggtgtcg
    44821 tctcgcccgc cgccacccca agctcgcgcg acacggcgtc gctgatgatc tgccccccgc
    44881 gcacgtggca ggccgtcccg agacacacct tgatcgagtg ccgccccggc ggcgtgaagc
    44941 ggaactgcga gtagaacgag gccacgccgt acacctcgtt ctccgtgatc cgcagggcca
    45001 gcgcgatctg ccgcaccccg tccgccgata tgaacccggc cgtgtgctga agtttctgca
    45061 gcagcgggat gaggtcgccg cgctcccccg aacattcgcg cagtatgccg ctgagatcgg
    45121 cggccgcgcc gggcgccccg gccgtggaag ccatgtgatt cattccgcac cacctcggtt
    45181 ggaaaggaac acgcaatcgg tgagccgggc gtccccgcgg gcgcagtctt cggcgaacgc
    45241 tttgcacgtc ggcgcgccgc agacgccgca gtcgatctga tgcagtttct ggaatatccg
    45301 gtcgacctcg cgcaggcgct tgatggaggt ctcgaggtcg gtgtcgaaat acttcgtcgg
    45361 ccgcggcggc accgagtgct ccagcgcaaa gtcgccgtcg gtcagacgcc tctcgatctc
    45421 ggcctccggg atcggccgcc ccttctcgta gcgggtgcgc tggcggatgt tgttcgcccg
    45481 cgccacgtag gggttctcga cgttgaaaat ccccccgaca caccccatca tgcaggccac
    45541 cgcctcgacg tagtcgacat tgcggaggcg cgaattctcg atgtcgtcga atatcttcat
    45601 cacgtggtcg agccccgaca ccgccagcca gttgtccatg ttgaggctct gcgtgatccc
    45661 gccgagaatc ggccatcccg cccggaaaaa gaaatcctcg ggcaccgccc cctcgtcgaa
    45721 ctcggcctgg aggcccatga cgatcgggta gagcagcgcg tacacgtcgc ggatcgccac
    45781 cgccccgttg aaccacgact gctccttctc ggccggctgc tcgatcgaca ccaccttggc
    45841 cggacacgga gatatgtaga tgatgccgat ctcctccgag cccaggcccg tcgacgccat
    45901 caggctccgc cgcgtccccc gcgccgtcaa ttcccgccgg acgtccaggc ggatgatgtt
    45961 acccaccagg tccgggtacc tcacctggat gagccggatc acgcacggac actcgctcga
    46021 gatgaccggc cgcggccctg tgtacttgtc caggtagcgg gtggtcgctt tcgccaggta
    46081 ctcgcaggtg agcccgacat tgaccacgag atcgaacccg agccgccgga acgcctggtg
    46141 aatgacccac gggtggatgc tcgaatcgaa ctgggcgtag agagcggagg aggggacgac
    46201 gactttgtac ttgaatccgc tgaacgcctg gagcgggttg atgaccgggt cgattgcggc
    46261 cgaggggcag accgagatgc acgtgccgca gtcgatgcag agttcgtcgt ggatgaccgc
    46321 cgtgccgttg cggacccgga tcgcctgcgt cgggcagcgc cgcatgcagt tcatgtgccc
    46381 gcggcacttg cggggattca cctggtgggc atggtagtac tggaccacgg cgcgtccttt
    46441 cactcgcgcg cgacgcgcgc tcagatgtaa aaccgcatgg tcagcgtcgt gcctttgccg
    46501 acctcgctct caacgttcaa ctcatccgcg ttcttcttga tattgggcaa tcccatgccc
    46561 gacccgaacc ccatcgcccg catctcttcg gtcgccgtcg accaccccgc ctgcatcgcc
    46621 ttctcgatgt ccggaatgcc cttcccccgg tcggccagca ccaccgtgat gcagcgcggc
    46681 gtgatcgaca gcgtggccac cgccgactgc gcgtgcatga ccacgttcat ctccccctcg
    46741 tacgtggaaa tcgccacccg ccgcacgagc ttcgggtcca ggccgatctt cttcaggatc
    46801 gacttcacct ccgtcgacac ccccccggcg ctggcgaaat cgtccccctg gatcgtgaat
    46861 gtctgggtga ggctcatctc ctccggcttg accgcggcgg gggagttgac cgcctgggga
    46921 ccgttgtgga gcacccggcc gacgctcagg cagccgtcga acatgtccag ggtcgtgctg
    46981 aggagcacca gaccgcggtc gcgcgccaac tcgtccgagc cgtcgatcgg ccgcttgccg
    47041 cgaatgaacg cgaccgccac gaactcggcc atgtgcgcgg aaatgatcgc ctgcttggtc
    47101 gccaggccgg tgaggagaat agaattgggc cggccgaaag caagcacatc gctcatcagg
    47161 tccgatgcat acacgtggtc gacctccaga tcgaggcgat ccgtgccttc cagcacagtc
    47221 gcgttcagtg cgtctcgaat cgcgcgaatc tgcatagcgg cggtgtgccg gtcaccgtcg
    47281 gcgcgtcgcc ccgccgggcc gccgcggcga gtcgtcgccc cggcgcgctc ctacggcgtt
    47341 ccgatcccca ggctgtacag ccgcccgacc agctcgtaca cgggcagcgg cgacgccagg
    47401 atggcgatgt tgtacttgtt ggccaggtcg atcgtctcct gcggcaccgt cttgccgctg
    47461 gtgacgacca cggctgcggc atcgaccaga ttcgccgccg gcacgatgtt cttgtgggtc
    47521 tgcaccgtca gccacaaatc ccccgacttc gccccggcca tcacatccga aagcatgtcg
    47581 gaaatgaaca cgcccgtcac cggacgcgga tcaaaccggc tcagcggctt cagcccgatt
    47641 ctctccacca gatcttgggt tgtcacactc gttctcctgc ctgtgggtgc gctgtgtatg
    47701 gaatcctcag aatagctgcc tccggccctg cggcggctcc agcgacagca tgcccgaatc
    47761 gtggatgaag tcccccttgc agagactcag gttcgaaatg aggcttgagg aaacgatccg
    47821 gatctgcgac cgcgtccggt cgcgcgattg attgaaatac tccgaaagta ccgccagctt
    47881 cttctccgaa gccggcgcgg aaagagcctt gggaaggtag ccgaaggcca ccagaaccca
    47941 gatgcgctgc gcgtgcagct cccgctgcaa aacctccacc gggcaatctt ccagacaccc
    48001 cgtgcagtgc agcaacccca tcaggtccga aaaatcctcc ttccgcccgc acagcgcgca
    48061 ctggagattg aactggaatg actgcgcaac ctccatccgc gtccaaaaac acacgccctc
    48121 gcgatacttc tcgtccggaa tgggattgtc ttcggtcgtg aaatacgtcc gcaggccgtg
    48181 gcagtgaaaa cacttctcga acaccacgaa gccaaactgg atgtctttca tcttccatag
    48241 atgctggcag tcgtacataa cgctccctat cgttcgagac gcctcgggac agcaccccgc
    48301 aatgcctgcc ggtagaggaa ggtaagcgaa atgcccctaa cactctgctg atgcaccttg
    48361 cctgcgatga tgccaaaccc ccccggccaa cctctcccgg ggggcaattc tcctaacccg
    48421 tctgatgtga agatgatatc tattagtagt atatgtcccc cccgcgcgaa aaggcaaggg
    48481 aaatctctcc gtctttatct gatttagcga caagtttgcc cagattcaac gctacaaaca
    48541 cgataaaagt cgtattcttt ttcctgcgac tccgatcacg acgcccaatc gcgcgccaaa
    48601 aaaaaaccgc tgtcgtcacg ggacgacagc ggctccaaaa actcgccgga ccacccggcg
    48661 actgttccca ccgagcctac ttcagaagca gcattttgcg cgacaccaca ccctcatccg
    48721 tcgtgatgcg atacaggtac acgccgctgg ccgccggtcg gccggccccg tcgcagccgc
    48781 cccacgaaac ggtgtgccgg cccgcctcca gccgtttatc caaaagcacg gcaacctctt
    48841 ggccgagggt gttgtacacc gtgacctgaa cccgggtcgc tcccgtcaga ctgaacgaaa
    48901 tgttggtctc cgggttgaac gggttcggat aattggtcac ctcagatacc ttcggcatgt
    48961 ccagcgagag ctgcacgatg agcgtgagcg ccgggctcac cccggccacg ctgattctca
    49021 ccgtgtccac atggatgccg ggcggcaatc cggccggatc gaggtagagg cacaggctgt
    49081 cggcggtcac tcccgtcggg tccggcagcg atgtgaatcg gccgaccgaa tcggccaccc
    49141 aggccgtgaa cggcgcgggc gcatttgacg acgtgacgag cgcgcacttc gtggtcgggg
    49201 gcagcatccc gtacggcccg aaatagtgca ccgtcgcggg cgtgaccgcc gcggtgggcg
    49261 cacccccgct gtccaccgtc agcgtcagca tcaccggcac cgtcaccggc agcgccacgc
    49321 cctccaccag acaggcaatg ctgtcgacat atgtcccggc cgagaagggc gccgccgggt
    49381 tgatgaccag ccgcaccgta tcactggtga cgcccgccga gtccgccacc gacagccagg
    49441 ccgagccgtt tgccgcatac gcgacatacg gggccggggc gttggtggaa ctgagaacga
    49501 agtggcagtc gatgaggtcc tgcaggccga acggcgcggt gatgctcacc gtcggcggcg
    49561 ccgcccaggc ggagggaccc gggccggcgg aatcgtccac gacgtaaaac accaccggga
    49621 ccgccgtcgc cgccgccacg ccctccacgc tgcagtcgat cgtgtcgaca tagatcccct
    49681 gcccgaggct cgccgcgctc gcgccgaaac acaccgagtc attggtcatg cccgaggcgc
    49741 cgtagagcgt cagccacggc gctccgtggc ggacgctggc cgtaaacggg gccggcgcat
    49801 tgctcgaata gagcatgaga cagcgcacct gcaagagctc cgaatactgc ggcgccgtga
    49861 gactgaaact cgcgggaatg gccaccgccg aatccccgcc cagcgtgtcc tccggcgcta
    49921 ccgatagctg gaccggcacg agcaccgaat cgggcacccc ggcgaccacg caggcgatgc
    49981 tgtcgctgta gaaaccgggg gccagcccgg ccgcgtcgac cgtgaaccgg accgagtcgt
    50041 tggttgtgcc ggccgcatcg atgacgtgca gccacggcga ggagaacatg accgcggccg
    50101 tgaacgccgc cggggcgttc gacgacgaca gtacgaacga tccggcgtag ggcggggtct
    50161 gcggatcgct tgcgaaactg aaccaggagg gactcgcata ggcgcttggc cccgtgccgc
    50221 ccgtgtcatc cagcaccgtg agcaggacct gcaccgcggc cggtatctcc acccccgtca
    50281 cgcggcagat gaggctgtcc cagtagaagc cgggggccaa acccgcgccg tccacggtaa
    50341 accggaccga gtcgttggtc gtcccgatcg agtccgccag gtgcagccac gacactccca
    50401 gggccgcctc gaccgagtag gacgccgggg cattggtcga cgccaagagg aacacgcccg
    50461 cctgcgccgg cccgcccgcc cagggagcat ggacaaactc aaagtaggtg gggtgggcgg
    50521 tggccgtcgc tccagccgtc gaatcctgca cctccagcgt gacatacacc gccggcgtgt
    50581 tcaccgcccc cgccacgtgg cagtaaagcg tgtcggtgta gagccccgga acaaccccgg
    50641 ccgccggcgc gatcgtgaat gatatggaat cgttcgtcac cccggccgaa tcgggcacgt
    50701 gcagccagtc ggaaccgccg gccgtctcga gatagtacgc cgagggggcg ttggtcgacc
    50761 ggagatatgc gcgcccctcc tgcggcgggc cgcccggcgg cgacgtgaag tagaagtaca
    50821 tcgggctgac cgccgccgaa tccacctggt cgccgatcgt cagactcacc ggctggtaga
    50881 ggtcgtacgt caccccgctc acccggtaca ccagcgtgtc gaggtgcacg cccggcgcca
    50941 ggccggccgg gtggaggagg atgcacacgc tgtcgttggt catgccggac ggggagagga
    51001 tctccacgaa cgacgccgcg ctcgtatcga tccgccgaac cgtgaacgcg gccggcgcgt
    51061 tggtggaagt caggtacgcg cacgcgtcga tcgtctgcac ccagccctcc gggagccaga
    51121 acgagtatcc ggtcggcctc accgccgcgt cctgtgagat gacggctgtg tcgctcacgt
    51181 acaggctcag cggcacgaag atcgggctgt tcgacacgcc gctgatgtca aactggatcg
    51241 tgtcggcgta gatcccctcg ctcagcccgg acgacgagag agtgaagcag atggttgcgt
    51301 tcgcgcccaa caccgagtcc gccgtgatcg tcgtgaatgc gacattaccg cccagcacgg
    51361 tggcgttgaa gaccgaattg gggttggtcg agtacaggtt gaaacagcgc tggaccgccg
    51421 tgttcggggg cagcgtgagc gccagcacgc cggggtccac cgaggccgta tcgataccga
    51481 ccgtatcctg cagcgcctgg agcgggttcg ccaaaccggc gcccagtttc ccgaaaaagc
    51541 ccgggttggc cgggtagatg ttctcggcag tcgcgatcag cgaggcgtgg aggctgcgcc
    51601 agtcgagcgc gccgccggct tgcgaaacct tgagcgccgc ctggcccgca acaaacggag
    51661 cggcgaaact cgtcccgctc caccaggcgt acatgtcgaa atacggggcg tagatccatt
    51721 cccccggcgc acagagatcg atgtggttgc cgtagcgcga aaattccgcc agccggtcga
    51781 gtgtgtcgac cgccgcgacc gccaccacgt tcggatcgga ggccagatag gtcggcacct
    51841 ccaaccgctc gttgccggca gcggccacga ccaacacatc gcggctgcgc gcataggcga
    51901 gcgtgttccg cagcacctcg tggacgttcg tcatcacgag gctgagattg accacccggc
    51961 agccgtcgtc gatcgcccga aggatcgcct ccgccacccg gtcgccgttg ctccgcccgg
    52021 aggtgtcggc cacccggtag gcgatgatct gcgcccccgg cgcggtcaga tggaccaccc
    52081 ccgccacgaa cgtgccgtgg ccgctgttgc ggccccccgg ctcatcgaac gcatcggcgt
    52141 cgccgtcgac atagtcccag cccgaaaccg ctgctcccgc gagcaccggg tgggcgaaat
    52201 tcacgccgcc gtcgaccacc gcgaccttga ccccggcccc cgtcgccagc gcgtgcgcct
    52261 gcgaaagacg cagccgcaga gccgcctcct gctccgggta ggaacccacg ttgtcggcat
    52321 cggagaaggg gaaactcccc tgcaccgact gcagcggcga caccacgtag ttggggtgtg
    52381 caaatatcac ccccggcagg ggccgcaggt tctccacgag agcctcgata tcggccgccg
    52441 ggtcggccgt ggcgaggata tagatgttga cctggccgaa atactccttc accgtcgtgc
    52501 cgaacagcgt gttgatcgaa tcaaccgaga tcgagtcgct gaacagcctc accaccactt
    52561 cgccgggaac atacggccgg aaagtcgtgt ccggaccctc cgtcccggcg ttggcgttgg
    52621 gcggaatcgc gcccggcggc tgtgccacgc ccggcccggg ttgcgacgac ccggtcaggc
    52681 cgatgaaggc agccgccagc gcgagaaaca gcagaaactt ccctgcggtg gccccggtcc
    52741 agtgcggtat cataggaacg cctcctttat ccaagaagct gcgggacatt tttctcacac
    52801 ccgatctatt ccaggttcgg cgtcagccgg cgccgaacag cgaaaacccg gcccaatcgt
    52861 acgccgaggg atactcttcc cgcgcgtccg cggctgcccg ctgcacggcc gcggcgggtc
    52921 cgctcccttc agcgagcgcc ccatagaacc gctcgatcca gcgcgcggtc gaggcgtccg
    52981 ccaccgccca gtggctggcc agcacgttgc gcgctcccat ttccagcagc gcgcgaatca
    53041 gcccggtcga ctcctcgccg ggcaagaacg acgacaaccc ggtccgacag gccgccagca
    53101 ccgccaggtt caccgtgttc cgccgcaggc gaaaatcggc ggcgaacagc ggcccgtcag
    53161 caagctccag gaaggagtaa aacggattgt cgctgcgaaa atgcgcatgg ccggtgaaat
    53221 gccagacatc ggcctccgaa tcaaccggcc agtcggcgcg accgcaggga ttgtgaaccg
    53281 ttgtctgcct gcgctcatcg gccagcagcg gggcatactc cgcgcccgcc gcgccaagcc
    53341 ccgcggcgcc gacaaatatc ctggtctccc gcgactgtgt ccgctgccgc gcggctgcga
    53401 ggtgatgccg cacgctcggc gccatcacca gcggatgccg gtctatcaat ggccttcccc
    53461 cgaccggcag cgccgcccag gggagattcg ccagggcgcc gtcggctacg atcagcactg
    53521 gctgcggccc gcccacgtcc agcggccgcc agagggtctc ggccaaccgg gcgaggagcc
    53581 gccgttcatc ggcgagatcg gccgcggttt caccggccgt gcgatagggc gcgcgctcga
    53641 caaaaaaccg ccatcgctgg aggaggcgct cgaccgccgg cgcggcgccc tccagtcggc
    53701 agccgcgcgg ccgcccctcc tcgtggatga ggccgatgat gtcgccggag acggcgtgca
    53761 actggacaag gcgcatggtc cgggaggccg cggcgatttc cgcgcccagc cggtcgagcc
    53821 gctccgctgc gctcccgcgc ctgcgctcca accgcgcaag gtcggtgcga atttgctcct
    53881 cgagctgccg ccgcaacgat gtcgccggcg ccgccccccc gccgcgcacc cgccggccgt
    53941 cggatcgctg cgccgccgcc gccacgtacc gcgccagcga ggccagcgac tgctccgcct
    54001 gcgtccgcgc cggatcgtcg agccgcgcat cctcggtcgc ccagatgccg gccgtgtgaa
    54061 agcgctccag ccaggccgcc gcctgcgagg gatgggcctc ccactcgcac gcgatcaact
    54121 tctcgtacgg ttccgcgtgg cgctcggaga aagccgcccg caggtcaagc ggggggagct
    54181 gggcgcgcac agccgtcagg atttcggcgg cccggcgcca gtggccggtc gccgcccggc
    54241 ggcggccccg ccgcgcctcg ctgtccccca gcagcgtcac atactgcgcg cgcacgtgcg
    54301 gcatcgtctc gaccgcgcgg ttggcggcca gccgccgccg cgcccctccg tcctccggcg
    54361 cggcgctgag aatctccaga tcgcacagcg cctcccagag cggcagccgc ccctgcggca
    54421 tgcgttcccg cagcgccgcc agcgcgcggg cgcgcgcccg ccccgattct ccggctaccg
    54481 cctccgccac ccgcaccgcg gcgaggaaac cttcgtttcc ctctcttcga aagcccgcgg
    54541 ccgccgtccg gaaagccgtc cggccggcct tcatccggcc gagcgctgcc agcgccttgc
    54601 ccctgaaaaa ctccgctttg gcctgctcgt agcgcaaacc cagccggcgg gccagcatcg
    54661 ccgcccggcg cgccgtgtcg cgcgcatccg gcaaaagatt caaactcagg taggtctctg
    54721 cgcgatcaag tacgcagagc aggtgctcgc gcgcctgccc ggccgcgcga tagtgggcct
    54781 cgcactcggc cagttcctgc agagcggcgt gaaagcggcc ctccagcatg tgcagccagg
    54841 ccagaccgta caggcagccg gccgcgcgca acctatgatc gtgccggaga aaaacctcgc
    54901 gggcggagga atagagcttc tccgcggcgg cgaaatgaaa aagctgcacg agcgtgttag
    54961 cctcgttgta ccggcagaac gccgccgcca gctcgtcccc ctggcttgcg aagaacgccc
    55021 ccgcccggcg gtagagcggc agcgcttcgg ggtgccggtc gaggcggtgc aggacgttcg
    55081 cgcagttgac ttccgtcttg gccagatcgg cctcggcctt gcgcccgcgg aacaccgcga
    55141 tcgcccggcg gccgcgccgc agcgcctcgt cgtgccggcc caggtacatg tacacgtcga
    55201 tcagtatccg gtcgatgcgc gcgcgggcga gcgggtcgcg cgtggcccgc cgcgcctcta
    55261 gatagcgcgc cgccgccggc tggtacttgc ccgccacgac atacgcccag ccgagtgcgc
    55321 gaagcgcaac cgggagcatc gtgcggtcgg cggcgcgggc ccggcggaca aactgctctc
    55381 ccagacccac cgcgcgccgg gctgatcgct ccgtttcccg acggacaagc tggtcgcagg
    55441 aagccgctaa ttcggccggc gtcctgccgc ccagccggcc cgtctggaga aaacgccggg
    55501 cggcgcgctc gcagtcactt ccgatattca ccatgacaca ttctcaaagc ggatttcccg
    55561 gtcgggtgag atcagcacaa ccgtccgggg cggccggctc gacgaccatg tcaggaaacc
    55621 gtccccgccc ggaaccacgc gctgccgccc cgcccggagc acgaaatccc ccgccgccgt
    55681 ccggccccga tacacccgcg cgatgaatcg ccactcccgc cgccgctgct cgccgacaat
    55741 ctccagatcc aggtcaggcg ccgccaggcg caaccgccgc accagcccct ccgggagatc
    55801 gcgaacggcg gccggccgct cccgccacga gtcgaacacc acccggccgc tgctcttcgt
    55861 gcggagtgac ggcggcggcg ccagcagcga aacgttgatc catcgctcca ccgcgtcctc
    55921 ggatggcgcc atcaacccgg cgcttcccgc cggagaaaag cgccgcagca tctcaaacca
    55981 tttgaggcac tcggcgcatc tctgcaggtg tccgcggagt cggccgggac ctgagcgggc
    56041 ggccgcaagc atttgcgcgc gcgtgggatg gcgatcggat gatttcatac ggcggtttcc
    56101 ttcactctct gccctaaaag agccgcaccg gccccgctta gtccaccggg tagcccagtt
    56161 cggcaaggat ttttcgcagg cgcgccagac accgcgagcg caacggtccc agcgcgttgg
    56221 gggagaggcc gaagtgccgg gcgatttccg cgtaggtcgt cccgcccggc gagaaaaaga
    56281 gcttccgcag caagtcccgg cagcgcggct cgagccgcgc tagcgccccc tcgagaatcg
    56341 ccgcctgctc aagctgtacc acctggtcgt cgggcagaca caaggacgcc gcctctggcg
    56401 cgtccgcttc cacctgacgg cgggctgatt gctgctgtcg cattcgcacc gcccgccgcc
    56461 gggtcgtctg gatcagccag gccggaagac gcgccggatc gcgcacattc ttccgcgtcc
    56521 ggtacagcgc cagccacacc tgctgcccgc agtcctccgc atcgggcgca ctcagcccgc
    56581 tgcgcaccgc caccgtcata actagcgggg caaactgccg cacgaggtcg gcccaggcgg
    56641 cccggtcgcc gtcgaccact cgccgccaga gaatgttgat actctcggtc acatctgcgg
    56701 ttatgcggac agccctcagt cagggaccgg ccgttgtcgg tgaaggatct cgttcgtccg
    56761 cgtatttata cgatccgccg ccgaaaaagc aacgccaaga acgcccggcg tgagatttaa
    56821 acctccgggg gtgcacgaaa ccgcgctcgc acggctcgca cctgataccc atatttcccc
    56881 gcgttgccct tgtcaaccga cccgtaaggt cgtatcttgc cgtcaatagg gcaagcccgc
    56941 tcgcagcgcc atgcggaccg gtaatgcagg atcggtgcgg cgaccatgac agatacaccc
    57001 gtggcctcga ttatcgcgaa cttccttcgc acgcccctct cccggcgcgc cttcgatgcc
    57061 ttcttcgacc tgagctgtcg ccgcactatt gtcttcctcc gcaacctgcg cgcccgccac
    57121 gtgcctctgc cgctggccgg ttatgccggc gatgatccca tgcgggacct ggccatcgac
    57181 gtgctggcgg gtatcttagc gccggccggc ggcctcccct gccttgggct cttcccctat
    57241 ctgaaacgcc agggctgccc ggcgctgccg cccgaggaca tcaccggcct cgaatccctc
    57301 ttcggcatct tcctctactg ccaggtgaag cagcagatct cgcgcctttg gggagaacac
    57361 gatccgcagg tcgcgctcct caagcggcgg gtcagggaca tcctcgcgtc ctcggagtat
    57421 gtctcctgga ccgatccctc cacccgcgcc gtgctggtgg cccctgctca ggatgccccc
    57481 catccgcggc ccgatgcctt tgtcgtcgag cctgacatgc tgcgccgaac cgtgctcgcg
    57541 gccttcaacc gcacgaccaa tcgatcctcc tggtgcgccg cgattttccg cctgatccgc
    57601 gatgagaccg gcgctccgcc ggtcgtcccg ctctatgaat tgctggccct gatggtcgag
    57661 gtgaacgccg cctttctcgc cggcgccgag tgcgcccccg cgcccctccc cggcccggaa
    57721 gaactctgca cccgcgccgc gggcgatcgg gcccgccaac gggcggtgga cgataccgcc
    57781 gcctcggcta tcgcccgcta tgtcaccctg ggacggatca ctccggccga gggagaggct
    57841 ttccgcaagg ctctcgagct ctacctcgcc gatttcctcg ccggcggctc cgacggcctc
    57901 ccggcttact tccgcgaggt gatgccgccc gaagcccacg agcgctacct gcaggactac
    57961 aaatatgtgt tcgaaacgac tctcaacgag gggtaccgcc ggatgatcga tttgcttggt
    58021 cagtccgaca tcggcctgct ccagtgacta ttttctacgg gagcatggaa ggagaagacc
    58081 gtgcacgtga acgtgactga tttgctcctg tttctcgacg ggaagctatc cggcgaccgc
    58141 agggatgtgg tgcaggggca tctcgctgag tgcgagttct gccgggaatt ccttgaagga
    58201 cttcaggata cgcaggttgt gggcgagtcc gaccgggagc cgatcccccc accggctatg
    58261 cagttgcgcg accgcatata taatgaggct ctgcgcggac gggtgattcc gatgaccccc
    58321 atggcgccgg ccgccccccc gcaggagtac ctcctggccg ccgatggccg cccggaggag
    58381 cggcccgccg tcgagtgcct tcgcgagtat ttctccgaaa gccccgaaat ggtcctccgc
    58441 gtcatgcgca gcgcccccga ccggcggcag tgggtccagt tggtgaccga tgatccccgg
    58501 caggctgcgc acgtgctcct ccgcctgccc gacatacaga aagagctcct caccgactcg
    58561 gccggccggg ccgacctctc cgatgtcgct gatgccgaca tcgaagcgct caagtgggag
    58621 gtgaagctcc ccgatgtctc gttcacgctc gatgaactcg cgtatgatcc cgaccggacc
    58681 gaatacggcg gggagaccgt tctcgaaagc tcccgagccg accgcgtgcg gctccgcttc
    58741 gagggaaaga cggaggggaa gctggtgacg ctcgaattcc tgaacctcgt cggcatcgac
    58801 gagaaggacg ccctccgggt gtatgtgatc gagggaaccg aggcccacgc gctcgaggcg
    58861 cctcaggccg gccgggcgtc gttccggctc tccggttccc ccaaaagcct ctcgattcgg
    58921 ttgtttaagg cctaggcgat acgatgaccc tctcgtccct gcagttcgcc cgcgcgttcg
    58981 gcgccctcaa agccgcgctc gccgcccgcc cggtcggcga ccatcgcctg ctcgcgctcg
    59041 ccgcgttctg gccgaacctc gccgcccacc gcgccaaccc gtcgtaccaa tcctacctcg
    59101 atgagtacac ggctctcttc gccgaatcgc tcgcccccga acatcttgcc gactgccctc
    59161 ccgaaaatct cgccgccatc cgcaccctcg ccagggaaat cggttccccg gaacagcggg
    59221 agaccgtgga cctggcgctt gcgcgggcct gggcgtatgc gggggagact gaacgagccg
    59281 tcatggtctg cgcccagggc gccggcgaag tcgcggcggc caaagagctc ttttcatcgg
    59341 gcgccgatgc caaccccccg atgacggcgc gggggagacc ctcgtgatcg agcgcgaccg
    59401 gctgcgggag gccattgacc ggcttcgcgc ccccgctccg gccaccgccg cgcgcctgga
    59461 caaaatcgcc gccgaatggg acagcgaaac cgggcacctc tggttcgacc gcgtccgctg
    59521 cctgtttgtc gaaaccgacg gcggcgaggc cacccgcggg cggatgtaca cgctcgtcgg
    59581 cgcggtcgag ccgatcggcg cgcggacccg ccgccccggg gatttggccg ccgatgaagt
    59641 cacgttcgag gggcagttgc gggcgccgga tgatcccttc gtcggtgtcg tgtacgactc
    59701 gctcaagtcg gtccgcccgc tcttgcgcgg tctggccggc agcgaacctt tcggcagcct
    59761 ctcccgagca gccggacccg tcggcatctt tccgcgcgtc gccaaagccg ctctgccgaa
    59821 tttgggcggc gatgacgccg cgagtctcca tgcccacttc cgtctcgagg gcaacagccg
    59881 caccgcgacc ggcgattcca tcggcctggc cgcggcgctc gtcgcctaca cgcagttgct
    59941 ccgcccggag attatgcgcc acgagcgatt tgtctcttcc gaggtggcgt tcaccggcag
    60001 catcgccgcc aatggaacgc tgctgccgat caacgccgcc acgctcgccg tcaaactcga
    60061 gcgtgcggtc ttctcgaccg tccggtacgt cgtgatcccg gccgccaacg aagcggaggc
    60121 ccgcgcggcg ctggccgctc tgggcgcccg ctatccgcgc cgccgccttg gcctgattgc
    60181 cgccgagcgc ctctccgatg ttgtggagaa ctacaacatt gtccgctccg agcggatgtg
    60241 catcggtcag ttcgtggccc gcaaagcggt gcactggggc cggagcatca agacggaggt
    60301 ggccctgctc gcgatactgc tctatgtgct tctggccatc acatggacgc gaaccttcgc
    60361 accatggtgg tttgatcata acccggtgcg atacgagatc cttggtaagg aattaaccct
    60421 cctaaacgcg gccggtgagc gtttgtgggc cacgacttgt cagtgcgagt cgatcctgcc
    60481 ggaatttgca tttgtgagaa tcgtggatgt cgattcggat gccgacaatg aagtggccgt
    60541 tcttgtgagt caggcttccg actgcccgga caatgccatt ctccgtgtct tcgatcagga
    60601 cggcgattcg ttgtggagcc gcagtgccat catcccgggc cagccggcgg agactctcct
    60661 cgcgtcctgg aattacgagg tggccgctct ttccttccga gatacgcaga acggcccggt
    60721 attcatgact gttctgaacg ccagcctgaa tggtacaagg agctacattc ggttttttga
    60781 ctctgtcggc gacccgctag gttggtacgt ccactccgga tatacgggaa tccactcctg
    60841 gttcgcagac tcagcaggcc ggcaaaagtt ggtgatgcag gggtggaaca acgcgtatga
    60901 tactaagtgc ctattcttgc tggatccgtt tagcgctcag ggatgttcgc ctcctttcaa
    60961 ccttatgagc gccgatctca tgttcggtgg agaaggcaac cagccggcat atgtcctgtt
    61021 tccattggag gagatttcgc gagcgctcgg tatccagcac tacggggcaa tggaaccgca
    61081 agttgtccac ggcagaatca aggtgacact cctccctata ccttcacagc ccgaggccca
    61141 actgatctat tacttcgatc aatctcttag ggttgaagaa cttgacgtgt cagagcagct
    61201 tgcgctgtcc tacaagggct tggtttcctc gggggcactc ccttccaaga ccctttctga
    61261 aggaattcgg gagcgatacg cgtcaatccg gtactggaca aatggggggt gggtgacaga
    61321 gggggaattg cgggcggccg ggaaataacc gggaattcgg gttccgacaa atctcgcgtt
    61381 cagtacctat tccctgagca aagacaattg ctgaaagcga ctagtgtgtc gcgccattcg
    61441 gcgcgaacag cgctcgaaga actcgttccg atggaggttt gcgatggatc tgaggaaaca
    61501 atctctcgtg gtgttattgt tggccttcct cgtctcctct ccgacctggg gggcaaccac
    61561 tatttcggcc ggcgggcatg ccccttccca cgccgtcatt gccgcaaacg gcgacctctt
    61621 gtctcccgcg cccgagcctt ggtgtgacag ctttgaggat tactccggct ctggctggtc
    61681 ggtcaactgg catgggagcg gcaacgtccc cggtattgaa atcgatcata catacgccca
    61741 caccggatcg gcttccttac gtatgttcgg cctgctgggc tcgtgttggg gttctgtcgt
    61801 cactcggccg atcagcatgg atctaccttt ggaagtcacg ttctcggtca caaatggcca
    61861 tgaaccgctc tcgggctgcc acccttaccg cgccatgttc gggcttcgca ccggccccga
    61921 ctggtgggac tgtccctgcc cggggctctt gacgttcctt ccgaacggcg acatcaagct
    61981 gggcccgtgg ggtggtgcaa ccgtgtacac cggctttgcc ctggacacct ggcatgacgt
    62041 aaggtgccgc ctctcgcttc cgggtgacag tcagttgcat gccgagttct gggtcaattc
    62101 gtcttacctg ggcgagatca ctctgcccga ggagtcgtgg atgagcgaac cggcctacct
    62161 cgatatttcg tgtcaggaag ggacggtctg gttcgatgac atttgtgtgg gaccggccag
    62221 tgaaccggag tttctccccg tcgccctcga catcaaaccc ggctcctgcc cgaatccgct
    62281 caatctgcgc ccctatcgcg acggggcacc gaccctcgca gtggatgagc tcagcgatgc
    62341 cgtgccgtcg gccaagcccg atcccggtgt ccggcctccc cgcggcgtcc tcccggtcgc
    62401 tatcctcggc acggccgagt tcgatccgac gatgatcgac tggacgacgg tgaccctgca
    62461 aggcgtctcg ccgatccgcc acagctttga ggatgtcgcg gccccggtgc cggccgatgc
    62521 ggtgcagtgc cagtgcacgg ccgccggccc cgacgggtat actgatatga cgctcaagtt
    62581 ctaccgcgat gagattatcg ctgcgctcgg ccaagtgtac gccggcgaga cggtggcgct
    62641 gactctgacc ggcaatctgc tcgacggcac accgatcgag ggcgtcgact gtgtcgttat
    62701 catcaacggc cccgagccgc cggagccccc gttggcggcc gacgatcaaa cgcccgtcct
    62761 gctgggcaac taccccaacc cgttcaaccc ggccacccag attgggttct cgctgccgac
    62821 cgcgtcgcac gttacgctcg tcgtctacaa catcatgggg cagcgggtgg cggtgctggc
    62881 ggatggtcag tacgacgcgg gcgaccacag tgtcacctgg gatgcttccg agcagtcttc
    62941 cggcatctac ctgtaccgtc ttgaagcgac cgggttcagc cagacgcgga agatgatgct
    63001 gctcaaatag tcagaggagg gacgggacgc gacccgaggt cgtgcggatt gagtaacacg
    63061 acctggggcg aggcgcccga caacaaacgc tgcggcgacg cagcaggtaa gggacagcgg
    63121 gggaactcac acgcaggccc ggtaagaaaa ccgggcctgc ggttggtttt tcacccccgc
    63181 tcccgccgac ctaatcacct tgcccaatcg ttcgtcatgg ccgatattac ccccatggta
    63241 catcgcctga cccgcgtcat cggttcgatt ggtctcgtgt gtgtgctggc gggcgcgctc
    63301 ggccgggccg acgccccttt tgcggtcctc gccccggggc ttgagatggc gactttcacg
    63361 ccggaccacc ggggagcggt ccgttcgccg atgagccgcg accgcaccgg cgtgaccatc
    63421 ctccgcatcg acccggccca gtacgaattc tgcctgctcg cgcaatccaa cggcgacgcg
    63481 ccgaccaacc tgagcgtggc cgaatgggcg cgccgccacc acctggtggt ggccctcaac
    63541 gccggcatgt acaacctcga ccagcgcagc cacgtcggct acatgaaagt cggcgaccac
    63601 atcaactgcc ggttccccaa cccctaccag tcggcggcgg ctttcggccc ccgcagcgac
    63661 agcctcccgc cgtttcgcat ctacgacctc gatgaggact ccctccgggg actggccgcc
    63721 tcctacacct acgtcatgca gaacctccgc ctgatcaagc gccccgccga aaaccgctgg
    63781 cagccccagg acaaacgctg gcgcggactg gccctcggcg aggataccca gggacgggcg
    63841 ctcgtgatcc tcacccggtc ggcctggtcc atgtacgatc tgaacgaatt cctcctctcg
    63901 ctcccgatcg gaatcgtcgc cgcccagcac ctcgagggcg gatccgaggc gcagcttttc
    63961 gtgcgcaccg ccgataccac ctttgccatc gtcggcagct acgacacctc gctcgacgat
    64021 gacgacggtc agtgggcggg ctggccggtg cccaacgtca tcggcatcgc cccgaaaacc
    64081 cgataacccg cccccgccgc caggccgagc gccccccggc caacttccgc aggacagggt
    64141 tctgcaggtc aggacctcta tggtcctgac ggggctgctt acatcgatag taagaatgtc
    64201 aggaccacaa gggtcctgac ctacaagaga tccgcagaat tgtctctgcg gatggagaga
    64261 gcgttgtgac cgccggcgtc ctgtcctact tccttttctt tggccggtag taatccgtgg
    64321 cggtgccgtg gaacaagtcg gccgcctcca tcaccgtctc gctcagcgtc gggtgcggat
    64381 gaatgctcag gccgaggtcg tcgacggtgg ccgccatctc aatcgccagc acgccctcgg
    64441 cgatcagttc gccggcgccg ggtccgacca cgcccatccc gaggaggcgc tgagagtgcg
    64501 ggtcgatgac cagctttgtg gcgccgtcgg tgcggccgag cgtgagagcc cggcccgagg
    64561 cggcccaggg gaagcgcgtg atctcgacct cgattttctt cccagccgcc tcggtttcgg
    64621 tcaagccggc ccaggcgacc tcaggatcgg tgaagacgac ggcgggtatg gcggcggggt
    64681 cgaaggcggc ttttcggccg gcgatcacct cggcggcgac ccgcccttcg tgcagcccct
    64741 tgtgggcaag catcggttcg ccggcgacgt cgccgacggc gaagatgtgc ggcgcgctgg
    64801 tgcgccgctg ggcatccacc ttgatgaacc cgcgcgcgtc ggtatcgacc cccaccgcgt
    64861 cgagcccaag ctgggcggac atgggcttgc gcccgatcgc cacgagcaca cggtcgaaca
    64921 actgcgcctt cccggcggcc tgcttccccg cgaacgcgac tttcaccccg ttggcctgca
    64981 ctttgagttc tctgacggtc gtctcaagca tgacggcgtc gaagatccgg tcgagccgtc
    65041 tgcggaggat ggcgacgaga tcgcggtcga ctcccggcag gaggccgtcg gtcatttcca
    65101 ccagactgac cctgctgccg agggcggcgt agatggtgcc gatttccagg ccgatatagc
    65161 cgccgccgat cacgagcagc gtgcccggaa tatccgcgag ttcgagggcc gaggtcgagt
    65221 cgaggaggcg gggggaatcg ggttgctgcg ggccgagggt ggcggggtaa gagccggtgg
    65281 cgatgacggc gtgttcgaag gcgaaggtgt attccgcgcc ggccacttcc ctgatcgaca
    65341 gcgtgtgggg atccacgaag cgggcgtggc cgcggaggtg gcggaacttg cgctgtttga
    65401 cgagctggcc gagtcccccg gtgagcttat cgacgacgcc gtctttccag gagcggagtt
    65461 tccggatgtt cacccggggc gcgccaaagg cgaggccccg gtctttcgcc tcctgcacct
    65521 cggccaacag gcgcgccgtg tggaggagcg ctttggatgg aatgcacccg cggtagaggc
    65581 agacgccgcc ggggttttcg gccggatcga tcagcacgat gtccaggccc agatcggcgg
    65641 cgaggaaagc ggccccgtag ccggcgggcc cgccgccgat caccgccacc tgcgccttgt
    65701 tccgagtggt atcgttcatg tcaggtctcc cttccggttt ggccgcgccg cggactactc
    65761 cagaaacatg gcgaacggac tctcgagcac gtcgcacacc cagcggagaa agcgcgcggc
    65821 atcggcgccg tcgacggcgc ggtggtcgta cgacagcgcc agcggcagga tcgtgcgcgg
    65881 ccggaactcg cgttcgatat agcgcggttc gatggccgcg cggctgacgc cgagaatcgc
    65941 tacctgcggc cagaggacga ggggggtgaa atcggtcccg ccgatccccc cctggttgga
    66001 aattgtgaac gtgcccccct ccagctcgtc gggcgcgacc cgccggccgc gggcgcgttc
    66061 cgccaggtcg gccagttcga ccgcgagggt gacgatgttc ttccggtcca catcgcgcag
    66121 caccggtacg agcagtccgt ggtcggtgtc gacggcgacg ccgatgtgga cgtagtgctt
    66181 gtagatgatc tggcggttcg cgggatcgag gctggcgttg aagcggggga accgccggag
    66241 tccctccccc atcactttca ggagaacggc cgttacggtg agcttcccgc cggcgttttt
    66301 cacgcggtcg gcgtttttgc ggaggaactc ctcaaggtgg gtgatgtcgg ctttgtccga
    66361 ctgagtgaca tgcggaatct gcgcccacga ccgggcggtg ctttccgcgg tcacgcggcg
    66421 gacggtcgac atgtcggccc gctcgatttc tccccaccgg ctgaagtcgg gcagatccgg
    66481 cgacccggcg ggagccgggg cgctcccggt cggtgcggct cgcccggaag cggcggcgcg
    66541 tcggacatgg accttgaggt cgctctcggt gatccggccg cccggtccgg tgccgctcac
    66601 ggcggagatg tccaggccca gctcgcgggc gagccgccgc accgagggcg aggccggagc
    66661 ggggccggtc ggccgaggcg gcgccgccgg cggcggagcc ggagcggaaa cggggcgggg
    66721 cgccggggcg gcggggggcg gcggagccgg ggtcttcgat tccggggcgg cgggcgcagg
    66781 ttcgcgttcg accgccgggg gcacgggcgg cggggcggcg ccggcggctg tgtcgagccg
    66841 ggcgatcacg ctccccactt tcagctcctc gcccacggcg acccggatct ccgtgatcac
    66901 gccttgttcc ggcgagggga tctcgaccac cgccttctcc gtttcgaact cgatgatgcc
    66961 gtcgtcgacg ccgacgatgt cgcccggctt gaccagcacc gccaccacgc gtcccgattc
    67021 gaccttctcg ccgatatccg ggatgacaat atccttgagc atatccctac ctcgtcacga
    67081 aaacaggggg ttggcccggc gcggatcgat gtcgagatcc ttgcgggctt ttttcacttc
    67141 ggtgaggctg agcttcttct cgcgcgcgag cgcggagagg gcggcgaagg cgatgtggcg
    67201 gtcatcgact tcgaagaaac cgcgcagggc cgggcgggtg tcgctgcggc cgaaaccgtc
    67261 ggttcccagc agggtgagcg ggccggggat ccacgacgcc agcatcgccg gcagcgcttt
    67321 gagatagtcc gatgccgcca caaacacccc ctgctcccct tccagcgctc tcgccagata
    67381 gggcaggcgc ggttctcttt ccgggttgag catgttccag cgctgggcgt cgatcgcgtc
    67441 ccagtagagg ttcttgtagc tggtgatgct ccagacgtcg gcggccacgc cgtaggcgtc
    67501 ggcgagccgc tcctgcgcct tgacggcctc gttgaggacg gtgccgctgc cgaagaggtg
    67561 gaccttgtgc ttcgcccgcc ggttgccgga ggcgcggaac ttgtagatcc ccttgaggat
    67621 cccctcctcc acgcccctgg gcatgtcggg catcttgtag aactcgttca tgatggtcag
    67681 gtagtacatg cggcgctccc ccttctccaa catgcgccgg aggccgtcac gtacaatcac
    67741 cgcgagctcg taggcgtagg cgggatcgta ggcgagcacg gtcgggtagg cgagcgcgag
    67801 cacgtggctg tggccgtcct ggtgctgaag tccctcgccg gccagagtgg tgcgcccggc
    67861 ggtcccgccg aggaggaaac cgcgggcctg gatgtcgccg gccgcccaga tcaggtcgcc
    67921 gatgcgctgg aaaccgaaca tcgagtagaa gaagaagaac ggaacggtgt tgaggccgaa
    67981 attggcgtag gcgctggcgg ccgccgtgaa cgacgacatc gcgcccgcct cggtgatgcc
    68041 ctcctccaga atctggccgt cggtcgcttc ccggtagtac agcagcgtct ccttgtcgac
    68101 cgggtcgtag ttctgcccct ggtgggagta gatgccgact tttcggaaga gggattccat
    68161 gccgaacgtg cgcgcttcgt cgggcacgat cggcacgata tacttgccga ggtcggggtc
    68221 ggcgagcagc ttgctcagca ggtggaccgc gaccatggtg gtggccacct cgcggtcgcc
    68281 gctgcccttg aggaactccg catagaggtc atcggccggg gcgggcatgg gcggcaccgt
    68341 gatgaggcgt ttcgggaggt agccgccgag ggcctcgcgg cgctccttga ggtagcgcat
    68401 ctcctcgctg tcgggggcgg ggcggtagaa gggggcttcg gcgagcgagg aatcgggcag
    68461 ggggatgccg aaacggctgc ggaagtggcg cagctcctgt tcgttgagtt ttttctgctg
    68521 gtgcgtgacg ttgcgtccct cgcccgcctc gccgagcccg tagcccttga tggtcttcgc
    68581 gagaatgacg gtgggtgaac cctcatgttc gaccgcctgc ttgtacgcgg cgtacacctt
    68641 gtgcgggtcg tggccgccgc ggcgcagttt ctggagctgc tcgtcggtgt agtgccgcac
    68701 catgcgctcg agctcggggt atttgccgaa aaaatcccgg cggatgtaat cgccggaggc
    68761 caccgagtac atctggtact ggccgtcggg cacctcctcc atgcgctgca cgagcagccc
    68821 cttgtcgtcc tgcgccaaga gcgggtccca gtcgtccccc cagatgactt tgatcacgtt
    68881 ccagccggcg ccgcggaagg ccgcctccag ttcctgaatg atcttcccgt tgccgcgcac
    68941 cgggccgtcg agccgctgaa ggttgcagtt gatgacgaaa atgaggttgt cgagcctttc
    69001 gcacggcggg agggtgaggg cgccgagcga ctccggctcg tcgagctcgc cgtcgccgag
    69061 aaacgcccag accttctgat cgtcgcggcg cttcaagccg cggtcctcga ggtagcgaat
    69121 gaaccgcgcc tggtagatgg cggagagcgg cgagaggccc atggacacag tcgggaactg
    69181 ccagaattcc ggcatgaggt aggggtgggg gtacgaggaa agccctcccc cgggggccag
    69241 ctcctggcgg aagttggcca gctgggcctc gctcagacgc ccctcgagga acgcccgggc
    69301 gtagacgccg ggcgccgcgt gcccctgaaa atagacgagg tcgcccggat ggttctcggt
    69361 gcgggcgcgg aagaagtggt tgaacccgac ctcgtagagg gtggcggtgg aggcgtaggt
    69421 ggagatgtgg ccgccgatgc cgctgtggtc gcggttggcc cgcaccacca tggccatcgc
    69481 attccaccgg ataatgctct tgatccgccg ttcgatctcg cggctgccgg ggaaaaccgg
    69541 ctgccgctcg accgggatcg tgttgacgta gggggtgttg gccgtgtagg gaaggtgcac
    69601 cccgttttcc tgcgcccgca tctgcagccg ccggagcagc tgccggaccc ggcccggccc
    69661 ctggctgcgg tggacgtagt ccagggactc aagccactcc cggttctcgt attcgatctc
    69721 cggcgcgcgg gtctggtcgt cgcgtcccgt catggcagcg cctttctgca tgctcgctcc
    69781 gcctcccgcc gctcccgtgc gacgggcgcg gttggatgcg ggggagaatt atcgccccgg
    69841 atcccggagc cgccgccgtc cgcgggcgtc ggccagggac cgccgttctg cctcggtggc
    69901 caaacataca tcgtaacgga ggcgggcaca agttgtttcc ggcggagaga gacctaactg
    69961 gatcaacgta atcgtcattc ccggccggcc gccataaagc ttgacgggcc gcgggcgccg
    70021 atgtagccta gggcgaatac ttaatagcga aaggagttcc gccatgcacc ctgcgccgaa
    70081 cacccggccg cccgctggtc ggcggctgcg ttttggccga aaggtctcgg ccctggtcct
    70141 cgtgctggcc gtcctggccg ctgccggggc ggcggccctg ctggccggct gtttcaccaa
    70201 cccggccacc ggcaaagaga cgttctcgct ctattccacc ggccaggaaa tccagatcgg
    70261 ccgcgaatcc gaccaggcaa tccgggcgca gctcggagtg gcgggggact cggcgcttca
    70321 gcggtacgtg gcggggatcg gccggaatct cgccgccgtg agcgaacggc gggacctgcc
    70381 ctggacattc tgcgtagtgg acgatccgac cataaatgcg ttcgcgctcc cgggcgggta
    70441 tgtctacgtg acgcgcggga ttctcgccca catggagaac gaggccgagc tcgcctcggt
    70501 catgggacac gagatcggcc acatcaccgc ccgccactcg gtcacgcaga tgaccaagca
    70561 gcagttggcg cagctcggac tggccggcgc cgtgatcctc aaaccggatc ttgagaaata
    70621 cgggcagttg atttccgccg gtctcggact cctgtttctg aagttcagcc gcgacgacga
    70681 gcaccaggcc gatctcctcg gcctccgcta catgtaccgg gggggctacg atccgcgcga
    70741 gatggacgat gtcttcgcga tgctggaccg ggtgagcggg cagcagggcg gaagcgcgct
    70801 gccgaactgg ctgtcgaccc atccctcgtc ggccgaccgg atccagcgca tcacccggat
    70861 ggtggattcg ctgcccccgg atttcagcgg ccgcaaagtg agccgcaacg agttcctcca
    70921 acggatcgac ggcctggtct acggcgacaa cccgcgcgag ggatatttcc ggggaaacac
    70981 cttcatccac cccgacctga aattccatat tgatttcccc gaccagtggc agacagccaa
    71041 ccagaagcag tcggtggccg gggtgtcacc caacgaggat gcgcttctcc aggtctcgct
    71101 cgcggaggcc cagacgcccg ccgccgccgc gcagcaattc gtgagccagc agggaatcag
    71161 cgcctcggcg ccccagggcg ccaccatcgg cggactcagc gccgccaccg cgaccttctc
    71221 ggcgcagacc ggggacggcg tgcagttgac cggcacggcg gcctttgtcg gctacaacgg
    71281 gcgggtgttc gaaatcctcg gctacggcct cgccggccag tggagccagt accggagcgc
    71341 ggtggccggc gccatagcgt cgtttggccc cgtcaccgac cagtcccttc tgaacgttaa
    71401 gccccgccgc atcgagatcg tgaccatccc gcgcgccatg acgatccagg agttcaatca
    71461 ggcgtatccg tcggattcca aaaccacggt ggaggaattg accatcctca acctggtgca
    71521 gagcggcgag aagattccgg cggggacgat ggtcaagcgg gtggtggcga agtagaggcc
    71581 gggaagcagc accgggaagg cggcggctgg gaggaggcat tggcctcttg tccatgtagg
    71641 tcaggaccct tgtggtcctg acattcttgc caacgacgca aacggcgccg tcaggaccat
    71701 gaagctcctg acctaccaaa cccctgtcct gcggaggttg gccgcgtgcg gtgtctttct
    71761 gatctcggcg gcctcggaac gcgcttcgca aaagcgccct ccccagaccc cgccgctgtg
    71821 ctgctcgctg tcggcgacgt ccatcaacgt gtggcgcaaa accgacaatc ccgccgcccg
    71881 ccgggcttcc gccgaccggg cgtgattacc ggcaaccaca tgccggataa tcgattatcc
    71941 ttgatttgtg cggccgggca ccggtgctgc tcagaatgtt cccgccgaca tccgcctccg
    72001 gatcggcatg aaacggtgga tgcctatggg tctgggaatt cgcaccgaca tcagcgcctg
    72061 ggatctggtc cgacggctca acgcctcgga actcgccctc tattcctcca tggagagact
    72121 cacctccggg ctggccatca accgggcctc cgacagcccg gcgatcctca tcctatcgga
    72181 gcgcctgcgg gcgcagacgg cctcgctcga gaggcagatc gaaaaccttg ccgcccggca
    72241 gcagaaatac gagtgcgcct catccgcgct catggaactc cgctcccacc tgaccgggct
    72301 gagatcgctg gcgctcggtg ccgccggcga ggggggccac gagccggcga tgcaggctgc
    72361 ctacgacacg gccgcgcaga atatcgtcga ggcctacaac cgggtgatct cgtgcgccga
    72421 gtacaacgga cggtatctct tcgatggcgc taccggttcg ctggcgaaac tcacgacgat
    72481 ccgcatcgac ctgtccgcgc cggccgcggc cgaatccgcg atcgccgcga tcgacagcgc
    72541 cgtccggcag gtggacacgg tccagatcga tgtgggggcg gcccagcgcc gcgacctgga
    72601 ggcgcagcag cgcatgttta cggccgcgcg ccgcgacctg gtcgccgccg aaagcgaact
    72661 gtgcgggacc gatgtcgccg ccgagtacac gagttttctc gccgagtcaa tccgcctgcg
    72721 ggccggcctg gcgctcctgg cccacagccg ggtgaccgcc gaaagcgtct tgtcgttgtg
    72781 ggatgactga ctccttctcc cccctgatgc ggcaatagga gtgccccctg tttgcaccaa
    72841 ccgccgggtt ccccaacccg gcggttttct gttgggccga tttccggcag caaaaaaacc
    72901 gctgtcggcc agcggttttc catatcgttc ggcagaaccg ccgcgcgccg cggcggttac
    72961 ttcgtagtct cggccggaac catcctgggg cgccgccccc ggacattctt catgaccttc
    73021 cccgccttga ggcaggatgt gcacaccgag aggtgtttcg gcacgccgtt gacgaccgcg
    73081 cggacattct gcaggttcgg gtagaaccgc cgcggcgtct tgttgtgggc gtgcgataca
    73141 ttccgtccgg tcgccggccg cttgccgcaa atatcacagg ctttggacat gactaccaac
    73201 tatctccttt cgcgccagag ccttttaatt tacaatcccc gccccaaaag gcaagccctt
    73261 tttgccggtc ggcgccattt tgccgccccc ccggccgccg tcaacctacc ccggccgggc
    73321 caccccccac ccccgcccgt cggcccccgc acccgaaagt gtttgttgca gcacaacttg
    73381 ttggtttgac tcagggaccg ggattgccta ttttggaatg aactggtgcc gccccgactg
    73441 gctataattc tgtagcagga tggacacgac cgatgcttga cctcaaattc atccgggaaa
    73501 acccggaggc cgtcaaggcc ggtattgcca agaagaacga ccgctcggac atcgacgcgc
    73561 tgctggccgt cgacgcccgg cgccgcgata ttatcagccg caccgaggcc ctcaaagccg
    73621 agcgcaaccg cgtcaccgcc gacatcgccc gcaaaaagaa agcccacgag cctgccgatg
    73681 acctcgtcgc cgagatgcgc cgggtcggcg accaaatcgt ccagctcgac gccgacctcc
    73741 gcgcggtcga ggacgacctc cagacccggc tttcatggat cccgaacctc ccccacgaat
    73801 cagtcccggt gggcggcgag gctgatatga aaatcgtgcg ccgctggggc gacctgcccg
    73861 cgcccgcctt cccggtccgc ccccactggg agatcggcga gcagctcggc atcctagaca
    73921 tcgcggccgc caccaaagtc tccggcgccg gcttctacgc cctgcgcggc ctcggcgccc
    73981 gcctcgaacg cgcgctcatc gcctacatgc tcgacaccca cgccgccggc ggtttctgcg
    74041 agcacgccgc tccctacctg gtcaccgagg agaccatgtt cggcaccggg cagttgccca
    74101 aaatgaccga ggacatgtac cgcaccgagg acaacatgtg gctcatcccg accgccgagg
    74161 tgtcgctgac caacctgttc cggcaggaga tcctcgacta tcgccggctg ccgatcctcg
    74221 tggtcgggca ttccccctgt ttccgccgcg aggccggggc ggccggcaaa gacacccgcg
    74281 gcatgatccg cgtccaccag ttccacaaag tcgagatggt gaaaatcgtg cgacccgaaa
    74341 cttcctacga cgaactcgag tcgctcgtcg ctcaggccga gaaaatcctc caggggctcc
    74401 gcatccccta ccgcgtcggc ctgctcgcct ccgccgatct ctcgttcgcc gccgccaaat
    74461 gctacgacat cgaactctgg gccgccggga tcgaacgctg gctcgaaatc tcctcctgct
    74521 ccaacttcga ggatttccag gcccgccgca tgaactgccg cttccgcgac gaggacaaga
    74581 aggtgcgctt tccgcacacc ctcaacggga gcggactggc cctcgcccgc cttgtcccgg
    74641 caattctcga aaactaccag aacgccgacg gttccgtgac tgtccccgaa gtcctccggc
    74701 cctacatggg aggcctcgcc aggatcggct gatgcccaaa ccgctgagca gattcagtct
    74761 gaccaaccgg ctctatgtcg gcaagtcgac cttctacaac ggcttcctcc tggtcggcgt
    74821 ggtcgccatc tcgctcgtct tcatctggta tacgctcaat gtcatcgacc gcctcaagga
    74881 gggcacccgc agccaggtgg agaagtacgt caagatgtgg cagcttgccg ccaactccaa
    74941 cacatcgggc gaggaactcc agttcatctt cgacgaaatc atcgtgaaag ccgatttccc
    75001 gatcattgtc ctcgaccaga accgggagcc gatccactgg cgcaacatca agggcatcgg
    75061 gccgaccgag aactcgccta aggcgctggc gaccctccgc cgcctggccg agagcatggc
    75121 cgcccgcaac caggaattcc ccctgcactt cggcgagggc cacgtcaact acttcctcta
    75181 cggcgacacc gacgccatca cccggctccg ctacatgccc ttcgtcgaaa tcagcatcgt
    75241 cctcgctttc ctcgccgtcg ccctcgtcgg ctaccacaac atccgccgca gcgaggaacg
    75301 ccacatctgg gtcggcatgg ccaaggagac cgcccaccag ctcggcacgc cgatctcctc
    75361 gctcatgggc tggatcgagg tcatgcgggc cgagtgcgac ccggccgccg accgcgccca
    75421 actgcaggcg cagtttgccg acggtctcga gaatatgcgc atcgacatcg accggctcca
    75481 gaaagtcgcc aaccgcttcg gccagatcgg gtccatcccc gagctgcacc cggccgacgt
    75541 caaccgggcg gttgccgacg ccgtcgagta cttccgccgc cgcctgccct tcgagggcca
    75601 gggcatcacc ctcgacgtcc gccgcggcga gctccccccg gtcgatctca accccgaact
    75661 cttcggatgg gccatcgaaa acctcatcaa gaattccctc caggcggtgg atcccaaagc
    75721 cgggcgcgtc ctcttcacca cctcctacct cccccaccgg cagtgcgtgg tgatcgatgt
    75781 cgaggacaac ggcgccggca tcccggcggc cgcccagcgc cgcatcttcc gccccggatt
    75841 caccaccaag aagcgggggt ggggccttgg cctgaccctc gtgcgccgca ttgtcgaaga
    75901 ataccaccgc ggacgcgtca cgctcaaacg ctcgaaaccc ggcgaaacgg tcttcgagat
    75961 cacgctgccc gcctccaccc aacgatagga tctaccatgc ccggatcggc cgaacaaaag
    76021 cgaattctct gggtcgatga tgaaatcgac tccctcaagt cgcacatcct cttcctcgaa
    76081 aaacgcggct acgcggtgga aggcgcggtg tcgggcgacg acgccgtcga gctcgtgcgc
    76141 cgcaaatcct tcgacctcgt cctcctcgac gaaatgatgc ccggcaagga cggcctgacg
    76201 accctcgagg agatcaagga aatccggccc cacctccccg tcgtcatggt caccaagtcc
    76261 gaagaagaga ctctcatgga ccaggcgatc gggcagaaga tcgacgatta cctcaccaaa
    76321 ccggtcaacc cgacccagat cctgtcggtc atcaagcgca tgctcgaggc caagcggatc
    76381 atcagcagca gcaccatgca gcgctacgtc accgaaatga accgcttcaa ccagaaactc
    76441 ttcggccgcc tcgagcccga ggactggtac gaggccggcc gcgtcctcgc cgcctggaac
    76501 ctcgaactcg accgccacgc cgacgtcgga ctggaacagg ccctcgaggg ctcccgcaaa
    76561 gagtggaaca ccgagttcac caaatacctc gaccgcaact acctcgactg gctccacagc
    76621 gaccgcgccc cgaccctgtc cccgcgcctg gccgaacgct tcctctttcc ccacctccgc
    76681 gagaagcgca agctcgtctt catcgtcgtc gactgcatgc gcctggacca gtggctgatg
    76741 gtcgagccgc tcatcgccga gtactacgac gtccgccgcg actactactt ctccatcctc
    76801 ccctcggcca cccccttcgc ccgcaacgcc ctcttcgccg ggctgttccc cgatgaaatc
    76861 gcccaggccc gccccgacct ctaccggacc caggccgagg gctcgctcaa ccgcctcgag
    76921 gatgaactct ttgaggacaa cctccagcgc cacggcttcg accaggcgcg ccagatgaag
    76981 tacgtcaaga tctacaacaa caccgagggc gaccaggtgg tccggcgcct cgccgacttc
    77041 tacgacgccc cggtcgccac cttcgtcttc aacttcctcg acatcctcgc ccacggccgc
    77101 tcgaacaacg tcatcctcaa ggaaatcgcc ggctcggagg ccgccttccg caccctcatg
    77161 cagacctggt tcatccactc gcctctcttc gccatgctca agctcttcgc cggccgccgc
    77221 gacaccaccg tcattgtcac caccgaccac ggctcggtgc tgtgccggcg gggcacgatg
    77281 gcccacggca agcggaccac ctcgaccaac ctccgctaca agtacggcga caacctcaac
    77341 tccgatccca atgactcgat cctggtcaag aaaccgaaag actggcgcct gcccctgccc
    77401 accctcgcca ccacctacat cctcgcccgc gaggacttct tcttcgtcta ccccaacaac
    77461 tacaacgaaa tgatccgcca gtttcagaac tcgttccagc acggcggcgt ttcgctggaa
    77521 gaaatggtgg tccccgtcgc caccctcaac ccgcggtgat atgcagacgg tgctgaacgt
    77581 ggtgtcccac tccgaaacgg agacggtcgg gctggcggag aaactggtcc ccttcctccg
    77641 ccccggcgac ctcatcgtgc tcaccggcga actgggcgcg gggaagaccg tcttcgtgcg
    77701 cggcctcgcc cgcggcctcg gcgtcgaccc cgctctcgtc aattccccct ccttcaccat
    77761 cgtcaacgag taccccgagg ggcggctgcc gctctaccac ttcgatctct accggatcgg
    77821 caaccccgcc gaattgctcg aggtcgggtg gaacgactac ctcgcccgcg agggaatcac
    77881 cgtcgtcgaa tggggcgagc gggccgggga ctgcctgccc ccccggtact accgggccgt
    77941 cttccgggtc gagggggaac acaaccgccg gatcgatatc ggcgtcgtgg gggacgaccg
    78001 gccatgagcg tgcagaacat cctggctatc gacacttcca ccgccctgct gcggttggcg
    78061 ctggcgttcg gcgccgaccg cctcgtccag gtgcgcgagg ccgtgccgca gtcgcacggc
    78121 caggtgctgc tgccgcgcct cggcgacctg ctcgccgccg ccggcctcac cgtccgcgac
    78181 ctccaggccg tcgccgtctc ggtcggcccc ggctccttca ccggcctccg catcggcctg
    78241 gccgccgcca agggcctcgt cgtcgccctc gacctcccgc tcgtcccggt caccgctttc
    78301 gaaatcgccg cccgccggct cgccgatgcc ccccgtcccg tctacgtgat cgtccccctc
    78361 aaccgggacg aggggatctg ctgcccgatc aacggccccc tcagcatcgg cccgccggtc
    78421 atcgcgcgct acagtgcgct gccggagatc gtcggccgcg cggccgtggc cgcgatcggc
    78481 ctgaccatcc gcgagaaaat gcccgccctg gccaaccccg acctgtccga ccggctcgac
    78541 tacgacgcgg gcgacctgct cgtcatcgcc cgcgaaaaac tggactccgg ccgaccgctt
    78601 cccgcggccg ccgaactcga accctggtac ctgcaaaaat cgcaggccga gatcaagttt
    78661 gagcaacgca aaaaaaaatg acctcgtcct ccgggacatg gccgcacgcg acctcgatac
    78721 cgtcgtcgcc atggaaaagg aaatcttccc cgatccctgg ccgcgctccg cgttcgagga
    78781 gcagatcggg ggcgacggct ggggggcgct tgtggtcgag atcgacggcg ccgtggtcgg
    78841 ctacgcctgc tacttcactg tcgatgtcga ggcccacctc accaacatcg ccgtccgccc
    78901 cgaacatcgc agaaaatcgg ttgcaaatat gttgctgggc gctatcttgg gtcgggcaac
    78961 cgcggccggt tgccagtatc tgctcctgga ggtccggtcc agcaacaacg ttgcccggac
    79021 cttttacgaa agaaaggggt tccgaatatt gtatcgccgg cccaattact accggcggcc
    79081 cattgaggat gccctggtca tggtcttcta cctcgacggc cggcaaggcg gatagacgct
    79141 atggcgtggt ttcggaaaga tcacagcggc ccggtgccgc aggaaaagaa aaatatcccc
    79201 gagggtctct ggatcaagtg cgactcctgc ggggaaatca tctacagccg aaaactcgaa
    79261 aaactcctct gggtctgccc ctcctgcgac ttccacttcc gcatcacctc ccagaaatat
    79321 atcgacctgc tgctcgatgg cgggcggctg gaggaattcg acagccacct gacgagcagg
    79381 gacccgctta agttcaagga ttccaagaag tacccggatc ggatcagaga ggcccaggcc
    79441 aaggccggcc gcctcgaggg tgtgatcgcc ggcatcggcg aaatcggcgg catcgaggtc
    79501 tccttcgcca tcatgaactt cgccttcatc ggcgggtcga tgggctcggt ggtgggggag
    79561 atgatcgccc gggccatcga acgcgccctc aaccgcaaca tccccctcgt catcgtctcc
    79621 tcctccggcg gcgcccgcat gcaggagggg attctctcgc tgatgcagat ggccaagacc
    79681 tccggcctgc tcgaacgact cgacaagaaa cgcatcccct acatctctat cctcaccaac
    79741 ccgaccaccg ccggcgtcat ggcctcgtat gcgtcgctcg gcgatgtcgt catcgccgaa
    79801 ccgaaagccc tcctcggatt cgccggcccg cgcgtcatcc agcagaccat caaacaggag
    79861 ctgccggagg gctttcagtc gtccgagttc tttctcgaaa aggggttcct cgacaagatc
    79921 gtgcaccgca aagacctccg cgacaccgtc atcgcgctcc tgaagttcat gtggagaaag
    79981 tgatgcccgc cggcgcctat cgcactgcgg aacggttcct cctctcgcgc gagttcttcg
    80041 gcatgaaact cgggctggag aacatcaccg agttcctcgt cgccatcggc tccccccagc
    80101 tgagctaccc gaccatccac gtggccggca ccaacggcaa gggctccacc gtcagcatgc
    80161 tcgccgctgt cctccgggcc caggggtacc ggaccggcct gtacacctct ccccacctcg
    80221 tctcgctgcg ggagcgggtg cgcgtcgacg gccatccgat cccggaacgc tccgtcaccg
    80281 cctttgtcgc ccgctaccgc cgggaactgg tccggcgcaa gttgtcgttt ttcgaggtgc
    80341 tcaccgccat ggccttcgag catttcgccc gcgccggggt cgacctcgcc gtggtcgaaa
    80401 ccggtctcgg gggacggctc gatgcgacca acgtcctttg tccgctcttg accgtcacca
    80461 ccgacatcag ccgcgaccac atggaaatcc tgggccgcac cctgcggaaa atcgcgttcg
    80521 aaaaagccgg catcgtcaag tccggcgtcc cccacctgat cgggctcctc ccgccggtcg
    80581 ccgaagaggt gattgcccgg tgctgcgcgg agctcggcgc ccccctgcac cgcctccgcc
    80641 cccgtgactt tgccgccttc ggtgaatcca accggctcga ctttcatgaa aacggctggc
    80701 ggatctccgg cctcgtcccg ggcctcatcg gcccccacca gttgcgcaac accgcgctgg
    80761 cgctcaaagc cctcgccatc ctgcgcgacc agggcctggc gatcgacaag caggccgtgt
    80821 accaaggcat ggcccggttg gagtggcccg gccgattcca gatcgtcgcc cgccgcggcc
    80881 gaccaaccct ggtattcgac gtcggccaca acgccggcgg catcgccgcc tttgtcgaga
    80941 ccttccagac ccgcttcccc ggccgcaagg ccctcatcct caccggcttc gtcaaacgaa
    81001 aagagcacca gaaaatggtc gatcacctcg ccaaaatcgc cgcacaatac gtcatcgtac
    81061 ccctcgatac caaacgctcc gtcgatactg cccacctctt ggggaccatc aactggcggc
    81121 gcgtgccggt ccaccgcgcc gggtcggtgg cgaccggctg gcgaagggtc gcccggaccg
    81181 ctcaacctga cgatatcata gctgtgatcg gctcgcacta ccttgtcgga gagtttttcg
    81241 agaagatcgg ctcggcatga tggagatgga ttcaaaagag cgccgccggc cggcgccgga
    81301 gacggccgat cccgaacgcc tccctcaagg ggaggcgctc cccgcgggtc ccggaccggc
    81361 ggccgcccgc gccgcggccg cggccgtcga actgcccgcc gaaaaactca cccgcctcac
    81421 cgaggccaac cgccagctca aacgcaaaat cttcgacctc tacaccatct tcgaaatcag
    81481 ccgcaacttc agctcggtcc tcaattacca gaccctcctc gattccttcg tcctcacctg
    81541 cgtggcccag gtctccgcct ccaagggagc catctacctc aagcaggacg cccgctcgga
    81601 ccgcttttcg ctcgccaaga gcaagggggg aggcgccttc ccccccaaag aggcaacctt
    81661 cccgtccgac tccaaaatag ccgactacct cgcccgcctg aaccgccccg tgccgacttc
    81721 cgagctgctc gccggcttct cccgggagcc ggaggccgag gctctccgta gtttcgatcc
    81781 cggcctggtc gtcccgctga tctaccagag ccgcctcatc ggactggtcc tcatcggcga
    81841 gaaaataggc gcgaaagagt tcggcctcga cgacatcgaa tttctgtcca tcctcggcaa
    81901 ccagatctcg gtggctatcg agaacgcccg gctctacgaa tccgaacgcg ccgccaccca
    81961 gcagttgctg gctgcccagc agcagctcgt gcacaccgag cgcctcgccg ctctcggcga
    82021 aatgtccgcc cgggtcgccc acgaggtcaa caacccgctc ggcatcatca agaactacct
    82081 cctgctggtc cagcgcactg cccgcggcaa cgtcgaaacc ggcaactacc tcgaaatcat
    82141 cagccaggaa atcgaccgca tcgcccgtat cgtccgcgaa ctcctcgact tccaccgccc
    82201 cgaaaaaacc gccctccgac ccatgaaact ggggaaggtg atcgacgacg tcctcgtcct
    82261 catggaccgg cagtttgaga aattcggcat catggtggtg cgggagttcg ccgatgacct
    82321 cccgctcgtc atgggttcgc ccgagaacat gaagcaggtc gccctcaaca tcgtcctcaa
    82381 cgccgccgac gccatgccca acggcggccg cctcgacatc gctctccgcc gcgttgccga
    82441 cggcgtcctg ctcgctttta ccgacaccgg cccgggcatc ccccccgaga tcatccccca
    82501 catcttcgaa cccttcttca ccaccaagca ggaggggaaa ggcaccggcc tcggcctggc
    82561 cgtctgctac ggcatcatca aacgccacgg cgggacgata acgtataaaa acagcagcat
    82621 cggggggtgc tttgaaatca tcctccctgc cctcgaggac cgcgagaacc atgggtaaca
    82681 ccggcgagcg gatcatcatt atcgacgatg agaagcgcat gtgcgattcg ctctatgccc
    82741 tcctcaccgg cgatggttac gaggttgaga ccttccagag gtcccccgaa gcggccgagg
    82801 ccatccgcac ccgcaaagtc gatctcgtca tcagcgacat caagatgccc ggcatggacg
    82861 gcatcgagct gctccgccgc gtgaaagagg tcgatgacgg catcccggtc atcctcatga
    82921 ccggttacgg gtcgctcgat accgctatcc aggccgtcgg tgccggcgcc tacgactacc
    82981 tcctcaaacc ggtcgaattc agctacctcg agctggccgt caaccgggcc ctcgaaaaac
    83041 gccgcgccga catcgcccga ctccagctcc tcgaagaact gcgcctctcc aacatgatcc
    83101 tcaagcgccg gatggatgac ctcaacgccc tctacgaggc cggcaagtcg atcggctcca
    83161 ccgccaatct ccacgaactc ctccgccaga ttgtcgtcct cgcctccacc gtcaccgagg
    83221 cccaggtcgg gtcgatcatg ctcctcgacg aagccggcga atacctcacc atcgaagccg
    83281 ccatcggcct cgacgaggac atcgtcgccg ccacccggct ctccatcggc tcctcgatcg
    83341 ccggctgggt ggcccgggaa ggagagcccc tcatcgtgcg cgacgtggag gccgacccgc
    83401 gcttccgccg catcaacaag gagcgctacg gcgccgcctc cctgctgtgc tgcccgctgc
    83461 ggatcaagaa caaagtcatc ggcgtcatca acctggccaa caaagccggg ggagagacat
    83521 tcaacgacaa cgacttgcgc ctcctcacga cctttgcctc ccaggccgcc gtggcggtcg
    83581 atgatgccaa ccagttcgaa cgcaaccgcc gccgcctcat cgaattcgaa atcctcaacg
    83641 agctctccgg cgaactgacc gagattcagt cgatgcacga tttctgccaa ttgctcgtca
    83701 ccaaactcac ccgcgtcttc ccgatcgatt gcgccctctg gttcaactgg gaacccggcc
    83761 gcaaaacgct cgtcgccgcc gccgcctccg gcggtcccga cctccgcccg gagcggcgct
    83821 ccgaggtcac cctcggcccg ctcgaactcg atgaagacat cctcgccgac atcccccgcc
    83881 tctccgccgc cgtcgctgcc gatatccgcg cccaccaggc gctgcccgcc ccggaaaccg
    83941 ccttcctcgc cctccccgtg aaacgccggg gggaactcgc gcacatcttc tacttcgccg
    84001 ccgccggcaa gaaaccctac agcgccgatg acatctcgat cgcccgcctg gtcgtctcgc
    84061 aggccgccct cctcttcgag cgcgagcagt cgctcctcaa catgacccgc ctgctcacca
    84121 tgggcaacat gatcagcgaa atctcccacg acctccgcaa gccgctcacc tcgatcaagg
    84181 ggagcctcca ggtgctggcc atgaaacacc cggagatcgc cgcccagtcg gaattgctga
    84241 atgcggccga agaggaggtc caccgcatga acgaactggt ccgcgaactg gtcgacttct
    84301 ccaaccccaa caagtaccag accgaaaaaa tcgacctccg ccgcatcgtc gcccgcgccg
    84361 ccgaactcgt cggccccgac ctccgcaaga aaaacatcgc ctactccgcc cgcttcgccg
    84421 aggccgactg ggacgtcatc gtcaacaaga accagatcct cgaagctttc cttaacctgc
    84481 tgatcaacgc gatcgacgcc atccccggcc ccggacagat ccgcgtcgag ggcctcctcg
    84541 aaacgccccc ccaccgcaaa gagccttacc tcgccgtccg ggtcgccgac tccggctgcg
    84601 gcatcaagaa agaaaacctc tcccgcattt tcgagcgcta ctacaccacc aaagacaccg
    84661 gaaccggcct cggactgtcg gtcgtcgaac gcatcatctc cgcccacaac ggcacgctcg
    84721 cggttcagtc ggaggagggg agaggggcca ccttctccct ctatttccct taccacaatt
    84781 gaccgctctg cacctttttg ctcaaaatcc gcaatatccc agacccaaaa ccttgatttt
    84841 gaccgccttt tgccgataca ttggctgtac ctgaatggtt gctatggcta aacagaagac
    84901 aaaaatcctg gtgatcgacg acgacccgaa ggtggcgtgg ctgctgtcgg aggggcttgg
    84961 ggcccgcttt gagtttgtcg ctgcccgcga cggctccgag gggctccaga tggtctcgac
    85021 cgagcaaccc gaactcattc tcctggatat caagatgccc ggaatgagcg ggatcgaggt
    85081 cctcgaaaag ctcaacaagc tcgagagccg ccccgaagtc atcatggtct ccggccacgg
    85141 cgacaccaac tacgtcgtcg aatccattaa gctgggcgcc gccgagttca tcaacaagcc
    85201 gttcgatgtc caggaaatcg aaatccacat caacggcgtc ctcgaacgcc gcaaccttcg
    85261 ccgcgaggtc aaagagctca aatccgaact gcaggcgcag tcggcctacg ccaatttcct
    85321 cggcgactcc gtggccatga ccaaggtgaa ggagatcatc gagcaggtcg ccgactccga
    85381 gctgactgtc ctcatccgcg gcgagtcggg caccggcaaa gagatcgccg cccgctccct
    85441 gcaccagctc tccgcgcgca aagaccgccc gtttatcaag gtcaactgcg ccgccatccc
    85501 tcgcgacctg ctcgaggccg agctcttcgg ctacgagaag ggggccttca ccggcgcgca
    85561 caaaaccaag caggggcggt tcgaaatcgc ccacaaggga acgatgttcc tcgatgaaat
    85621 cggcgacatg cccatggagc tccagtccaa actgctccag gtcctcgaac agcaggagtt
    85681 cgtccgggtc ggcggcatca cctcgatcca cgtcgatgtg cggatcatct gcgccaccaa
    85741 ccgcaacctc gaacaggcga tccacgacaa agaattccgc gacgacctgt tctaccgcct
    85801 gaacgagatc accctgttcc tgccccccct gcgggaacgt cccgaagata tccccctgct
    85861 cgtgcggcac tttatcgaca aatacaacca gctctattcc aaagacttca gagagctctc
    85921 cccctccact atcgaccgcc tcatgacctt ctcatggccc ggaaacgtcc gccagctcga
    85981 gaacatgctc aaacaggtgg cggtgcgcgg cgatgagtcg atcattatcg acctgatcaa
    86041 ctccgcggcc gccgcaccga ccatgccctc ccgctccacc tacacccctg ccaccaccga
    86101 cgtcggcccg actaacgccg ccgggaccga gacctactcg ctcaaagaac ggatcggccg
    86161 caccgtcgcc gaggaagaaa agcggttgat cagcgaagtc ctcacccgaa ccaactggaa
    86221 tcgccgcaaa gccgccgacc ttctcgagat aagctaccgc tcgctcctct ataagattaa
    86281 agactacaac ttgaattctt cgaagtagtc gttccggcga tctgtccaaa aacgtacccg
    86341 atgcccgcct gcgtcttata tgcatcggtg tgcatattct gcacacatcc tttcttattt
    86401 tccacatttt tcactccatg tgaataacat taagttgttg tgaaataacg tgtaagttgt
    86461 tgaccgcgac aaatctcggc acaagtttcg gaaatgtttt ccgcaatccg gcatcggggt
    86521 tgcatgggga acaaaacttg cttgtaaaat agtgcacaga agcgcgatag cgacgaataa
    86581 cgacgtggtg caacgatcat ggatgactgc aacttcattg acaaggtcga cctcgagctc
    86641 aaacgagccg agcgatattc ggtcttcgtc tcgctcgtcg ttttcgacct ctcattcctg
    86701 gagaaccact taggccccgg cgcctcctcg gtcgtgcatt cgctctcgca ggaggcgcgc
    86761 cgcaatgtcc gggaaatcga cgtcgtctcg gtgatggatg gctgtaagct cgtgctcttg
    86821 ttgcccgaga cgccgcgcca gggagccgaa atcgctagcc gccgggtcag cgagatcctc
    86881 cgggatcgcc tctcccggga caacagcgcg gcggcggaga aaatcatccc cctcgagatg
    86941 gcttcctacc ccgatgccgc cggggcgcgc accatccggg atttccttgg agactatctc
    87001 gatcggcaca ggaattaaga gaagcgggct gcgttcccgc acgtaaacaa gacccccctg
    87061 ccttttttgt ttctatgcct cccatttcag aagatcgcct gacccccagg cgatcttcat
    87121 cttttttggg gtccttcccc ggccgccgac acgcacggcc atgagaccgg ctactcggcg
    87181 caccacagca gcggcgccgg ccccccgcga aagaggtagg agatcatgtg ggtcagatcg
    87241 gcgaccgtca ccgcgcaatc acccgtcacg tccccggttc ggaattcaca gggaaggctg
    87301 ccgccccgga aaaggtggct gatcatcaac gtgaggtcgg acaccgcaac atcgtggttg
    87361 tcgttggcgt cccccggcag ccaggtccag aacgacgtca ccgccgactc cgtccgctgc
    87421 cccgtgttgt cctcggttgc cactttccac cagtatgcgg tccccggcgc gagcgaatcc
    87481 gccggcgtcc aggcggtgtc ggcgagcggt ccgaacacct gcagggcgac aaaccccgag
    87541 tcggtcgcca ggtgcaggaa gtagacgatc gtgtcgagcg ggtccgggtc gagcgagcgc
    87601 gaccaggtga atgtcgggag caggtccagc agcgagcagc cgctcgtgat cggggtcgtg
    87661 tgcggcatcg gcggtggctc cggcgcgtcg ttcacccaga acaaactgcc ggtcgaatcc
    87721 gtccagtccg aatactcata tccgtcgaac gcctgcgccc gccagcggta gcgccggttc
    87781 tccgtcagcg aaagcggcgg ctgccagccg gtcgagtcga tcccctccgg cagtccggcc
    87841 acgtactgcg agtcctccgt gcgcggcacc gtatcgagaa aaaccacaaa ggagtatgcc
    87901 agcgagtccc cttcggcgtc ggccgcattc gtcacgcgga gcatcggccg cgcgcccgtc
    87961 gagctgcctt cagagggcgc cagcgccgcc aattcgccgg gccgcgaatt caggcgaaac
    88021 gacgcctcga accattcgga ccaggccgct ccgttggcgg cgcgcagccg cacccggccc
    88081 gagagcccgt ccgccagcgg cgcgccggcg tacgcgaccg tcgtcgaagg cgaatcgaac
    88141 acggtgtccg cccataactc cgccgtcgtc cagtccgaat cgagaccgac cgcgatctcg
    88201 aaccgtgtct gcatccgtgc ctggagcgag tcggtgtagt gccacgcaac cgtcggcgca
    88261 tttcccacca cgtgctgcat gtccgtctct gccgcgaaca ccagcgcggc gataaccgga
    88321 accgaatcga ccgcgatcag ggtcaccgtc agcgaatccg ccagcgccgt gtcggccgcc
    88381 acgatgagga cgtcgtacac cccgttctga gtcgagtccg gggtgaaact gagccgcccg
    88441 cgcccgttgc cgctgtcgac aaagaccgcg tgcgcgggaa ggccctcggc ggcgagaacc
    88501 ggcacatccc cgtcggcatc gccggccgat accggaatcg ccagtgtgcg tccctcgatc
    88561 acccactgcg agtccaccgg gtcaagcacc ggcgccacat taatatcccg caccgtgagg
    88621 cccaccctca cgctgtcggc ggccagcgaa tcatcggtcg ccgcgatcca cacctcgtac
    88681 gcccccgcct ggcggtagcc cggccgccag gagaaaccgc ccgcgccgtt gccgctgtcg
    88741 acaaaaaccg cgccttccgg aataccctcg gccgcgagga tcagcttgtc gctgtcgtcg
    88801 tccgctgcgt agacccggaa ctcaagctgc tccccttcgt ccaccgcctg gtcgccgatc
    88861 gtatcgagcg ccggcgggtc gttgaccggg atgacgtcga tcaccaccgt ccgcgtgtcc
    88921 gccttgtacc cgtcggagac gacgagcacg aacagcgtgt ccgaggcacc ctccggcggc
    88981 gtcccgacca gggtgttctg gaacacgctg caccatcccg gcgccgcccc aaaagaaatc
    89041 accgtgtccg ggccgtccgg gtctgcgaat tccggctcat acccgaacgg ctggtcttcc
    89101 atggccagga ccgccgccgg cgagagaatc atcggccggg cgtcgagaca cccgactccg
    89161 aaagccccga tcacctcccc gcaggggctg tgcggcggtg cgcagggcga gatgtcgaac
    89221 aacccgaccg ccggattggc cgtgtcgcac agatggggat tgaggctgaa attgccgtcc
    89281 aatccgaact gcggcgcaat atcgcccgtc cagtcgccac cgctgttgcc gtacaggtcg
    89341 ctgcacgaca gcgtgaccgt cccggtcgag tccgcccgcc cgaccggcgg gcagtcgcgg
    89401 ttgaacaccg ccagcccccg gctgatcgcc accgttgcgt ccggaccgca ccacaccgca
    89461 gagaaaccgc tgttcccgcc cccctcgttg cgggtgaacg tgcacccggc gagcgtcgcc
    89521 gccgacccgg aatcggcgca cacgccggtt ccaaaccgcc ggttcccgcc gagcgtcgcg
    89581 ttgctgtcga aaagacaacc gtcaaacgac cccgtcgacc cgtacaagta caccgccccg
    89641 ccgaacacct cccggttcaa cccgtcgttc cggttgcgct cgaagaggca cttggcgaaa
    89701 accggaccgg agccgttgcc cgccgtcacc gccgcggcgt gggacgcgta gccgccgttg
    89761 tcgacaaagc ggcagtcggt cagcagggga gaggaggcat cgcacacgag cgcgccctgc
    89821 gagaggtcgg ccgtgttacc gacaaagtcg cagttatcga accgcgccgt cgcgccgttt
    89881 gtgcaccgca cggccgatgc tcgtcccagc cacggcgccg cccggtcgtc cccgctcttg
    89941 caggtcgaac acccgcccgt gttcccggta aacgtgcagt ggtcgaaagt cggcgaaccg
    90001 ccctcgcaca ccaccggcga gccggcgtga aatccgctga cgacgcagcg gcgaatcagc
    90061 ggcgaggatc cctggatcag cagcccccgc ccctccgtcg cggtataact gcccgagtcg
    90121 acaaacgtga acccctcgag caccgttccc ccggacgccg agtcaaagag aaaacaccgg
    90181 gtgaaccact gctcccccac ggtcagccga aatatgatcc gcgtgctgtc cgggccggtg
    90241 tccgagcgga tcaccaggtt cttgccccgg gtcgcgaggt cgagattccc cggccccgag
    90301 tagtttcctt tggccacgag caccgtgtcg cccccggccg ccgagtcgat ggccgcctga
    90361 attgtcgggt agtcctcggg cacatggcgt gtcgccgcac ccgcggccgg cacagccgcg
    90421 agcagaatga cgatgacgat agacactcgc accatgataa ccttcgagtc agcggatctg
    90481 agagcgttgt cggggccgca cccaatatag ggaccgccgg gggaaggtca atgtcaatcg
    90541 gcagccgggg aactaatccg cccgcccggc tccccgccgc gggtcctccc ggcgtcccct
    90601 ccgcatcccg cggaaagcgg ctgccccccc cgaaagagat aggcgatcat ccggccgagg
    90661 tcggccaccg agaccgcgca gtctccggtc acatcgagcg cggcctcccc gcagggcaac
    90721 acccccccgc ggaagaggta cgccgccgcc agcgtcacgt ccgccaccgc gatcgttccg
    90781 ccgccgtccg catcccccgg cagccacgtc cgaaacgtcg ccgccggcga catccgcacg
    90841 tgcccggaag aatcctcggc cgaaaccttc caccagtacc ttgtcgaccg ggcgagcggt
    90901 tcgctgaaga caaagctggt gtcttggaca cgggggcttg cccgcgccgc ctcaaaaccc
    90961 tcgtcgggcg ccagatacac gcaataggac accgcctcac ccgggttcgg atcggtcgac
    91021 gcgccccaca caaacgtcgg atagagcgtg agtggcgggc accccgggtc ggccggcgca
    91081 tacggcgccg gagccgtcgg cgtctgaaga tcgatcaccc gcatggtgag cgccgtgctg
    91141 caccaggtgt ggcggtcggt tgtgtcccgc gctcccgaca ccgccaggcg gtacaccgac
    91201 gtgtccccga cccgcaggac cagcggacgg acggtgtaca cagtcgaatc gattatcggc
    91261 gtccccggcc gctcgctgct acgggggatg agcggctccg ggtgggtggt gaagtgctcg
    91321 aggtcgctgc tgacgccgaa aaacaccggc tggtcgtcca ccagccctcc cgtcgcgaac
    91381 ccctcccagc gccctgccct cggcgaatgg agtgtcgtgt gccagtgctg gttcgggtgc
    91441 ggcagcgtcc actggaccac cgcgagcgcg tggtgctccg ccgccggatt gccgatcact
    91501 ttccgctgcc ccacccactc ggccggcgcg gcgggggaag ttatcggatc aggcgtctcc
    91561 cacagaacta cggtgcgccc ggcccccgtg tcttccaccg tgaatatccg gtagaccgcc
    91621 gcctcgaccg tcagcgacgg cgcccacagc cccagcgagg cgtgcaccgg cgccagcgga
    91681 ctcccgcggt tgtagtagcc gttgatcaca tgcgcagtgt cgatcgcccc ggcctgcgtc
    91741 caccggaccg cgtacacgaa attgacccag cgctcatcgc cccccagttc gtagcagacc
    91801 cgcgataccc gcacgagaaa cgacttcgtc ccctcccggt cgaggtacat gaacgggtcc
    91861 gacgtatgga cacagcggca cgaatccgcc caggcaaaca ggctgttgtc ggggacgcgg
    91921 aagatgctgt cggggaccag cggattgatc agcgtgtcgc cgccgggcgc agccggcgta
    91981 tgccagacgt caaggtcgag aatgccctcc gaccccgcgt ccaccgaggc atcgagcagc
    92041 cacaccgaca actgctccgt gtcgactccg ccgctggtga gcggcgtgcc gtaggcgtag
    92101 atcccctcgc ccggcaccgc caccagcccg ctgtggagcg tctggaaact gtcggcgggc
    92161 gtcagcaccg ccgatgcgta cacgcgcagg cgcaggcggc cggaggggat cgggtcgatt
    92221 tcgaccgtcg ccgccgacac cccgcccgcc agaagaagcg cgagtatgat tactgctctg
    92281 cccatgtgta ctcccgtcgg cggactccgc cgaccctcgc cctcaatata cgcggattcg
    92341 cggaatgggc aatcgcgaac cggccccgcc gcgccggcgg tccgggccgg aggcaagctc
    92401 ccgtcttatg tatcggctgt cccgggccgc gcgcccagcg cggttcgcgg ggggcgtctt
    92461 cgacggccgc gcccggcaaa aagaaggccg ccgggcctgc ggcccggcgg ctgccttgtc
    92521 cccgcggggc gccgccccgc cggccctcct accagcccat gatctgctcg aggttcttgt
    92581 tcagcttctc gatgcggtcc tggaaatccg ccttcttcgc cttctcccgc tcgatcacct
    92641 cggccggcgc gtgggcgaga aaatcctggt tggccagctt cttggacacc ttctcgagct
    92701 ggagggcgag gttctggagc tcccgctcca cccggttgcg ctccacctcg agatcgatca
    92761 gcccctcaag cgggacaaat atctccgccc ccgtgatgac cgccgtggcc gagggactgg
    92821 gccgcttgac cgccgtcccg caatggaggt tctccacccg cgccagcgac cggaaatact
    92881 cgatgtgttt ctgcagcagg tcgccgaact gcgggttgtc cacccgcacg tagaggtccg
    92941 acttgcgccc cggcggcacg ttgagctccg aacggaccga gcgcacggcg ttgaccaccg
    93001 cctggatctg ctcgagactg ctctcgagct ggtcgtcgat gaaccgtccc tgggtggtgg
    93061 gccacggacc gaaaacgatc gtcgtcttcc ggtccacggc ctcgagatcg gccccgatca
    93121 ggtcgaggta gatgcgctcg gtgacgaagg gcgcgaacgg gtggagcagc ttcacgatct
    93181 tgtccagaac gtaggtcgcg acgtcaagcg agcccggccg gatcgcctgc cccggctggt
    93241 ccggcttgat cagctcaatg taccaggagc agtagtcgtt ccagacgaaa ttgtacagcg
    93301 tcttggtggc cgccgacagc cggtactcgg cgaaattccg gtcgaccgcc cgaatcgtcc
    93361 gctccagccg cgagagaatc caccggtcga aaaggaccag gtcgctctcg tcgatcgtgt
    93421 cccgcaccgg gggacggccc tccaggcgca tcatgacaaa gcgcgacacc tggtgcagct
    93481 tgttgacgaa attccggccg acctcgaacg tattcttgct gatccacggg tcctgcccgt
    93541 cgggcgtcgc cagcgtcagc gagacccgca gggcgtccgc cccgtacttc tcgataatct
    93601 cgaccgggtc gatcccgttg cccagcgact tggacatctt gatcccgttg gcgtcgcgca
    93661 ccgtgccgtg gatgtacaca tccgaaaacg gcagctcgcc ggcgaactcg tacccggcca
    93721 tcaccatgcg ggccacccag agaaaaatga tctcgctggc ggtcgagaga accttggtcg
    93781 ggtaaaactt ctccagctcc gccgtccgct ccggccaccc catggtcgag aacggccaca
    93841 gccacgatga gaaccaggtg tccagcacgt cctcgtcctg caccagcgcc tccggatcgc
    93901 agccggcggg gagatcctcc ttccgcggcc gctcggccga cacaaacacc gtcccgtcct
    93961 cggcatacca gatcgggatg cggtgccccc accagagctg gcgcgagatg caccagtcgc
    94021 ggatgttctc catccagtgg aggtaagtct tcgaccagta ctccggatga aaccgcagct
    94081 tgcccgtctt caccgcctcc accgccggct gcgcgagctg gtccatcctc acgaaccact
    94141 gctccgacag gtacggttcg acaatcgagt ggcaccggta gcaggtcccg gccgcgagcc
    94201 ggtacttctc gatgcggtcg atatgcccct tgcgctggag ctcctcgacc agctgggtgc
    94261 gtccctcgaa ccggtccagg ttcttgaact tgccggcgtt ctcattgagc gacccgtcgg
    94321 cgttcaggat gttgatctcg gggagatcgt gccgcttgcc gatctcgaaa tcgttcgggt
    94381 cgtgcgccgg cgtcactttc accactccgg tgccgaactc ggggtcgaca taactgtcgg
    94441 ccaccaccgg gatgtcccgc tcgagaatcg gcaggatgat cgtcttgccg acgtacttct
    94501 tatagcgcgt gtccttcggg ttgaccgcca gggcggtgtc cccgagcatc gtctccgggc
    94561 gcgtcgtggc caccgtgagg tactcgtccg accctttcag cttgtacttg atgtaccaca
    94621 gatggctgtc aaactcggcg tgttcgacct cgtcgtccga cagcgacgtc tggcaggagg
    94681 ggcaccagtt gacgatgcgg aacccccggt agatccagcc tttctcgtgc aggcgtcgga
    94741 acacctcggc caccgcccgc gacagccccg gatcgagcgt gaaccgcgtc cgcgaccagt
    94801 cgcaggaaca gcccatgcgc ttgagctggc tgagaatctt gtccttgttc tggttggccc
    94861 actcccaggt ccgctcgaca aacttctccc ggcccatctc ccggcgcgtt gtcccctcct
    94921 tggccacccg cttctccacg atcacctggg tggcgatccc cgcatgatcc gacccgggaa
    94981 tccactccgc ctcgtacccc atcatgcggt gacgccggat caggatgtcc tggatcgtgt
    95041 tgttcagcgc gtgcccgagg tggaggacgt cggtcacatt gggcggggga atcacgatgg
    95101 agtacggctt cttgggtgaa tgcggatcgc cgacaaacac ccccgcctcc agccacttcc
    95161 ggtatatgcg gtcctcaacc tccgccggtg cgtaggccgc ggacttcttg atttcggtct
    95221 cgttttgctt gttcatgccg ctttcattcc atatattcgc cgaccggatc gaaccttccg
    95281 gcgggcggaa cgtaaaccgc ctagtatact ggcgcccggg gcgtttgtca aggcgcgaaa
    95341 ggcgtccgcc cgcgtgccgc taagtgcgct aattacttat actatcgtca gatatggaca
    95401 caattaaaga ccgcgtcctg gatccccgcc gacccccgtt cgaacgcctc gtcgctctcc
    95461 tcgctatcct ccgctctccc gagggatgcg cctgggaccg caagcagacc catcgcagcc
    95521 tcctgcccta cctgatcgag gaaacctacg aggtggtcga ggccgtcgaa aacgacaacc
    95581 cggccgccct ccgcgaggag ctcggcgatc tcctcgttca gattgtcttc cacgcccagc
    95641 tcgccgccga agccggccgg ttcactatcg acaactcgat cgccgacgtg gtcgagaaac
    95701 tggtcgcccg ccacccccat gtgttcggcg aacgaaaaaa cctcaatccc cgccaggtac
    95761 gcgaccagtg ggaacagatc aaagtcgagt cgggggagaa ggaccgggtc ctctccggcg
    95821 tccccaaagc catgcccgcc ctgactatgg cctaccgggt gggcgagaaa gccggcggtg
    95881 tcgggttcga ctggaacagc cccgaagagg tgttgggcaa aatagaggag gagattggcg
    95941 aaatccgcgc ccgactggcg gtcgccgaac ccgagggagt ggctgaagag attggcgacc
    96001 ttctgtttgc cgccgcctcc ctggcccgaa aagcggaggt cgaccccgaa cgggcgctca
    96061 agcaggccct cgacaagttc cgtcgccggt tcgaacgact cgaagatgca gtgaacggct
    96121 cgggccggag gttcagagac tactccctgg aggagctgga agccatctgg cagtcgataa
    96181 agtagcgctg cagccgctta ttgctatggg agtaaacctt tcaatgagaa tctcatttcg
    96241 caattcgctg tacaacaaga gattgcgaca gcggcatcct gctgagacga aggtttgaca
    96301 agtctttttt ttgttgaatt tccttgcccc tccaattaaa attggctcga tttttttgaa
    96361 ggcaccgagg ccaattgtga tgatgataga tgttataggt ggtcgtaaca ggagtgcgat
    96421 cgtgacggcg gcaatggccg ccgcaggagg tacgcctatg gtgtgaaggt agggatgagg
    96481 tcacgaagcg gctgccgcgc tcgagcgacg ggctggcgag ccgaaacccc aatcccgttg
    96541 ccgaggcagc ggtaggtttc ctgatgacac gaatcacaac taaggataaa cggttatgaa
    96601 gaaactggta atgctttcga ttctcgcgct gtgtgtgctg agcacaagcg ccttcgcgac
    96661 cgaaacgcga accctgaccc tcggtcagac caacaacgtc ctgttggacg aagccaacat
    96721 ttggctctac ccgagccgca tcatggatta cccgaacctg gcgatcggcg aattcggcta
    96781 tgaccccgac tccgacccga tcgagagcag ctctccggct gacttcaccc gcttcggcgt
    96841 gcatttcaag atcggccgcg acaacccgtg ggtgctcggc gcctactttg acaacctccc
    96901 tgcgtctgag cctgagaacc tgttcgatga cggcaacctt gtcgagttcg ataccctctt
    96961 cgatgacggg tggaacaacc ggcgcatcaa cctgttctac ggcaattcct tgggcaatat
    97021 caacttcggc gcccgcctcg ggctcctcca cagcagcgtc tcggccgaca tgaccgacaa
    97081 cgaggccaaa gaggggttct cctactatga cctgaccctc ggcctgaccg cccccgatgg
    97141 cggctgggac gtggccggca acatcgccct cggctctttc accgatgaca acgaagacgg
    97201 cgatgcggag aacgagaagg acggcttcta cgacctcagt ctccagggcc gcttcttcta
    97261 ccaggtgaac ccgagctaca cgattatccc gcacgccggg ctggcctaca gcaagcgcgg
    97321 gattgtaaac tacaccaacg acgatgatgt cgacagcgag gactactcca tcagcaccag
    97381 ccgcaccggc ctgcaactcg gtgtcggcct caactacacc cccgccacca acgtgctggc
    97441 tgtggccgac ttcggcctcg gctacaacaa ggtgaaggag gaattcgaca ccaccgacac
    97501 gtatgtcacc gaccccgatc tccccgaata caccgagcgg aacaagaccg acgtctggct
    97561 gccgtatttt aagctcggcc tcgacgccga cgtcttcaag tggctcgaca tccgcatggg
    97621 cgccgtgagc atctggaatc gccagagcga tgagaccaag ctcgccgagg atgtcaagta
    97681 caaggccaac tacgccgaca acagcgccta cctcgggttc ggattccact ggggccgctt
    97741 ccacgtcgat gcctggaccg atccggagtt gttcctgaac gggttcaact tcctgtcggg
    97801 cgccgacagc ggcgagatga acttccagct ctcggctgtc tacgaaatga tgtaatcgac
    97861 acccgcgaga tgacaccgaa aagcccggtc ccccgaccgg gcttttcttg tggatatcgt
    97921 gttccgggtc acggagcgct gtgacaacaa gccggtgccg gtccaccgcg gaagagaaac
    97981 gcggtacccc acagctccgc tgcggggact tagcgttatg cactcactgt cttgtccaac
    98041 atgtccgcct gcaggagcaa gtcatgccgt cccaggcagg aattatagcc cgaccatttc
    98101 ccgtcgccag ggcaagcacc cacagcagag ctgtgggcga cgagccctac cggcacctca
    98161 ccccaaccag ttatccagca accggaaatt cgtcccccga atcgtctcgt acgccccctc
    98221 gaactcggcg acaacctcct ccttcgtttt ccccagtacc gccaactcgt ccggatacgc
    98281 ctcgcaccag cgcacaacat cacatgcccg cggctccaga tgaaactgca gccccacctg
    98341 gcggttcctc cggaacgcct gatgtgccca cacctcatcc tccgccaaat gcaccccgcc
    98401 gaatggcgtt cggaacgtgt cgttatgcca gtggaacatg gcaaactccc gcggaaaccc
    98461 cgcgaacagc ggatcggccg ctcccgcctc ggtgagtttg gccatgtagg tcccgatctc
    98521 ccgcgcgttt ccctcctcca ccggcgcccc cagcacgcgg gctaagagct gcccgccgaa
    98581 acaaatcccg agatacggcc ggtcctcccg aacgactcgc gccagccagg caaagagggc
    98641 cttgagataa acatgctcgt gataccgtgc cgccgagtgc gggcagccca gacagatccc
    98701 cccctcgatc gcgtccgggt cgggcagcgc ttctccctcg taggccttga ccagcataaa
    98761 cggccgcccc cggctctcca gccattcggt tatggtgccc ggcccctccg ccgcaacgtt
    98821 ctggatcagc aggatcggtt tggtcatggc gcgtcctccg tgggccgaaa ataacccccc
    98881 gccgcccgct tgtccagcgg caacccgcgc gtaaccaaaa acgcgcgccc gaagcgcgcg
    98941 tttcgctgag tctgccggcc ggcccggggt caatcggccg cgatctcttt gacgaactgg
    99001 gggagagcga acgccgcgtg gtggacctcg gcgttgtagt acttcgtcgt cggccgcagc
    99061 cgggcgtgat catccgcccg gaaatcccgg atcggatcat acttcttgct gcagaacgcg
    99121 aacgaccaga gggctgaggg gtagatcggc atgaagcagg tgtacatgcg gacaatcggg
    99181 aaaatctccc gcaggttgcg gtacatctgg tgcaccgtgt cccggttgta aaacggcgat
    99241 tccgactgcg ccaccatgat gccgtcgtcg gccagccgct cgtggacagt ctggtgaaac
    99301 gacttctgga acaggtcggc cgccggcccg atcgggtcgg acagatccag gagaaccacg
    99361 tcgaaccgct cggtcgcctc ctcgatgtac cgcttgccgt cctggtacac cacctgcgcc
    99421 cgccggtcgg ccagcccggc agtgagggcg gggaagtgcc gtttcgacac ttcaaccacc
    99481 atccggtcga tctcgcacat cgtgcaccgc tccacccccg ggtgcttgag cgcctcggtc
    99541 aggcagccgc agtcgccgcc gccgatgatg agcacctgcc gcggccgcgg gtggacaaag
    99601 agcggtacgt gggcgagcat ctcgttgtag gcgttgtagt cgcggtcgca caccatgagc
    99661 gagccgtaca gcaccagcat cttgccgaaa tcaaacgtct cgagcacatc gatccgctga
    99721 tactccgact gcaccgactc gaccatccgg tcgaccttga tcgtcagccc cgactgcccc
    99781 tcgtgcagct cgctgtacca gacattccag agatccttca tcccctcttc ggcgatgtag
    99841 acctccccgc cggtcttctt ctgtcccatg ccgctgccct ctccggcctc agtcgatgac
    99901 caggtattcc ggccgccgca gggcgttgaa gttggagccc gatacggagc agtaggcccc
    99961 ggtggcggtg aagacgatgc ggtcgccgac ctcgtgcagc ggaatcatca gcccgtcgta
   100021 catgacgtcg aacgaatcgc acgtcggccc cgccagcacc gacaggaact gctcgccttt
   100081 cttgttcgtc accaccgggt attggcagtg gtcgaacacg atccccgaaa acgtcgagta
   100141 caacccgtcg tccaggtagt accagatctt cccgtcccgg tacgatcgcc cgaccaccga
   100201 ggtcaccagg gtgaccggcg tagccgcgat gaaccgtccc ggctcgctca cgatccggat
   100261 gcccggccgg atgtggtcgt cgagcgcttt ggcgatcggc gccgcgaacg cggcgatcgc
   100321 cggcaccggc tcgacatact cgaccggaaa cccgccgccg atatcaagca gccgggtgtc
   100381 gaagccttcc tcgtcgagcg cgcggatgag cgcgtgcgcc gtcgcgatcg cgttgacgta
   100441 gttgtcgctg tagacgcact gcgagccgac gtggaagcac aggccgtaga agtcgtgccc
   100501 cgtctcccgc accaggcggg ccagcggcag cacctcgtcc ggcatacacc cgtacttgta
   100561 ctggagattg accaccgcac tggtgttgac cgcggtgcgg tagcggatca tgaccttgag
   100621 cttcttctcg gcgtagcgcg tgaacttggg gacctcggcc ggattgtcga ccacgaacag
   100681 ctccagcccg cgtccgaccg cgtaatcgaa ttccggaacc gatttgatcg ggtgggagtg
   100741 gatcagggtg gccgggtcaa gcgaggtccg gcgcaggacg aggtcgatct cgcccgccga
   100801 acaaacgtcg aaattgccgc cctgtccgaa cacctcgtcg ataaagactt cgtggttgtt
   100861 gggcttgacg gcgtagtgga tgtcgacccg cggcagcgcc cgccggagtg cctggatatt
   100921 ccgcacgatg gccgaccggg acaccagaag cgcgggcgtg tcggcccgcg cccccgccag
   100981 cagttcgcgc agaagaccct ggtcgccgcc cgtcgcccgg gcgcgctgct ccatcacgga
   101041 cacatcaaca tgaggcgtca tcgcgtgtgc tccctttcta ccgcagggcc gccgcggcct
   101101 gcgtcggccg gcgccgggat gagggcttgt gatcgataat ctcgtcggcg tgcgagggaa
   101161 tcccccgctt gaactcgctc acctgcgcgc tccccgcctc gagctccccc ttcacgtgcg
   101221 ccagcgcccg gtgcggatcc accgactcgc cgcacgtgaa aaaatccacc gccgcatacc
   101281 cgtactccgg ccacgtgtga atcgaaatgt gggactcggc gatgaccact actccggaaa
   101341 caccttgcgg attgtaccgg tgaaacaccg atcgcacctt ggtcgctccc gaacggcgta
   101401 ccgcctcgtc caggcactct tcgaggaaac tcaggtcatt gagcttttcc tggttgcact
   101461 cggagagttc aactaccaga tggcgaccaa gaattttcat ttcgtagcct ccttctcggg
   101521 gtacgcggtt gtcccgtcac atatgcggcg gtgttgttac ccacagcagc ttggcggccc
   101581 gctcgccggc gttgccaatc tgatgtcgtt ggtcggactt gaaataaaaa cagtgttctc
   101641 gcggaacctt gaaactcttg ccatctatcc gcagcgtgac cgtgcccgcc agtacaaaac
   101701 cgaattgctc gccgggcatc ggatcccgca gttcgagaaa ttcccccggg ccgagctcga
   101761 gaagtatcgg atccatcagg ttgttcgtcg acccgggcat caacaactca aaacggcgcg
   101821 cccctttccc ctccacattc acccgctccg aggggcgaaa caccacgcgg cgtttctcca
   101881 catcggagaa aaattccccc agacccacac ccagggcctc gaggatatcc gccagcgaat
   101941 cgagattgat cgaactctga tcgttctcga tctgcgagat gaatccccgc gtcagccgtg
   102001 cccggctggc gagttccgcc tgcgtcaggt ccgaggccag ccgcagcgcc ttgatctttt
   102061 cgccgatcgt gaccttcatc ttctgccgtt aaacatcccc atcggcaaaa tgttcagtaa
   102121 aagttacacc gatggtctaa taaaattggc agaaaataac acctgccccg gaaatgtcaa
   102181 gacattattt caccctcccg gaactttttt ttgttatcgg agaattcaaa ctgattctgc
   102241 gccaaccggt agcccgcctg accaaaatga aaccccacaa gccccctccg ggttctgcaa
   102301 attccacgcc gagatcatcg gtttcgggca acaaagcgcg gttaaaacgg ctcaccgtgt
   102361 cattttattg cgacggccgc tcccgattcc gcccgcccgc aacctccccg gccccggcct
   102421 tgcccccggt ccggggcgcc ccgacggact gattcgcgcc ggaaccggac gagacagccg
   102481 ccccggattc attaatgtgg gagagcgcgc gctcgggtgc ggcccggctg accggtccaa
   102541 ctcgcgaggg gagaggcatg ggcctcgccg ccacacgcgc cccgggacgg gctaatctct
   102601 tgccgtacaa aagatgccca tcggatctcg tcgccggctc cggcccgccg gccggagcgc
   102661 accgcaaact ctgccgcccg tggccgggac cgtcctctgg cgaaatccgg ccgggccgcc
   102721 gcccgcgcca gccgcgacca acaaacgaac caacaaagcg gcccgccggg gccaggcgcg
   102781 gtatttcccg atgatccttg ccattcgccg ggaatccctt acattatgcc ccaatacagg
   102841 cggcctaggt caaaaccagg agattcagtc atagagaagt ttaatatcgt catcatcgga
   102901 agcggccccg ggggatacac cgctgccatc cgggctgctc agaagggggc cagagtcgcc
   102961 gtggtcgaac tcggcgaact gggcggcgtc tgcctcaatg tcggctgcat cccctccaaa
   103021 gccctcatcg cctcggccaa tgagtaccgg cgcatgagag acgcggagct ctgggggatc
   103081 cgcctcgcct cgccgccggt ctacgattgg accgccatgc gcgcccgcaa agacagaatt
   103141 gtcaaactga tgaccggcgg gatcgcccag cttttcaagt cccacggggt cacccacctc
   103201 gccggctacg gacggatcac cggcgccaac caggtcaccg tcgtcgatga ccagggcaac
   103261 gaatcgaaac tcaaaaccga caccgtcatc atcgccaccg gctcgcgctc gcgcaatctg
   103321 ccggggctga cggtcgatgg ccggcggatc ctcaactcca cccacatgct cgagctggac
   103381 cacctccccc aatccctcct cgtcatcggc gccggcgtga tcgggtgcga gtgggcctgc
   103441 atggccgcgc tgctcgatgt gcgggtgacg gtcgtggaaa tgctcgacca ggcgcttcct
   103501 ctcgaagacg ccggctcggc caaactcttc gagcgcgaac tgaaaaaact gaaaatcgac
   103561 ctgcacacca aaaccaccgt ggaatcgctg accccggccg gggagaccgt ccgcgccaat
   103621 ctctcgggcg gccaggcggt cgaggcccat caggtgctgg tggccgtcgg acgggcctat
   103681 aataccgaag atatcgggct ggaagaggtc ggggtgaaag tcaacaagaa cggctcgatc
   103741 cccaccggcg acgatatgcg caccaacatc ccgaacatct tcgcgatcgg cgatgtccgc
   103801 ggcgagatcc tgctggccta tacggcggtg cacgatggcg cggtggcggt caccaacgcc
   103861 ctcggagaga cgacaaccaa agactaccgc ggcgtccctt cggttatttt cactcacccc
   103921 gaagtcgcct cggtcggtct gtccgaggcc agggccgccc aggagcacga tctggcaatc
   103981 gggaggttcc cgctgcgcgt gctgggcaag tcgcacgcgg aaaacgaaat cgccggcgag
   104041 gtcaaagtca tcgccgacaa aaaaaccgac cgcctgctcg gcgtgcacat agtcggcgcc
   104101 catgccaccg aggtcatcca caccgctgcg ctggccatcc gcaacggact caccgcccgc
   104161 gaactcggca acctcatctt cggccacccg gtgatctccg aggcgctcat ggaggcggcc
   104221 cacgatgtcc acgggatgtc ggtgcatctg gctaagaaac gctgaggcgg ccgagccaac
   104281 gaacgaaccg gctgttttcg ccgaggcggc gcaaattctg ccggcgattc cgctcggata
   104341 ccacgcttgc gcagaaccca tcggttaagc tgattgcatt cgccactgga tgccaattac
   104401 ttaacatttg tttgtccaat gcgtgaccgc acgtcggcgc agctattgcg cttgccgctc
   104461 cggcgggttc ctccgggtcg tgtgtgtact atggtaagga attgtcactc agtcggttat
   104521 gggaacggcg caagagtgcc gagattccgg catagatctt gtatgttagt tctacgaatc
   104581 ggatgctctt acatagtcga catggcggtg aagggtatgt aagaggtggg gattgatggg
   104641 agtcacttcg agagagtgac tcccgttttt ttgcccgcca tcttctcccg tgctggaccg
   104701 cgctccggca atcgtcctcc cgcccgatcg cggctgcggg ctgaatgccg ccttcacatt
   104761 tgcccgtgca ataatgatcc cgccccgcca caggcgcgga ctgcaaatca agtgcggcct
   104821 gagttctcga cctgtttcaa ggagatcacc gacgctcaga gcggtggccc cgccggaggg
   104881 ggcattgccg ggcagtatcc gccctcaagc ccgcagacct cgccgttccc cccgcccggc
   104941 gtgacattag cgccgggttt ttcaccgccg gacacggaat tatcgtagcc gcaccctgta
   105001 aatcggaagt gcgcttgaca ggagatgaaa ttgtgttaaa ttccatgtct gcagccccgc
   105061 cctgggcggt gcgctgcgga tacgttcgac acagatggcg gtgtagctca gctggttaga
   105121 gcagcggaat cataatccgc gtgtccgggg ttcgaatccc tgcaccgcta tttctcttgc
   105181 cggtcggaaa atgcaggggg cgagcgcggc gccttcccag tacctcgccg gtctcggagc
   105241 acagcccaag cttgccgacc gtcgcccgac cgcgtatact tcacctcgaa aaagaacccg
   105301 ttgtcgacta tccccggacc ggaccgccgg tgcgggctta aggaccagca tgccaggaca
   105361 catcgccaga acaaccgggc ggattgccgc cctgcgaaca gccgggtgga tcgccgccgc
   105421 cagatcaact ggccggatcg ctgccgtcag aacagccggg cgcagcaccg ctgccagaac
   105481 agtcgggtgg atcgccgccg ccgcagcggc cgcggtgctc ggatgcagcg gcgccgacga
   105541 caccgccaag gtcaaacaga accccgcatc ggagggcgcc gtgaccatcg gcgtgtcgct
   105601 gctgacgcgg acccatccgt tctatcagga gttggaggag ggtttgaaag aagcggccgg
   105661 ggcggccggt ttccaggtgc ggattacggc cggggagttc gatgtcgccc gccagaaaga
   105721 ccaacttcag gatttcctca ctcagagagt cgatgcgatc atcgtgtgtc cctgcgactc
   105781 tcgttcgatc ggcaccgcga tcaaggcggc caacgaggcc ggcattccgg tgttcacggc
   105841 cgatatcgcc tgtctggccg agggcgtggc gatcgtgacg cacgtggcct cggacaatgt
   105901 cgagggcggg cggctggccg cccgcgcgct cgccgaggcc atccacggcg cgggcgcggt
   105961 cgcgatcatc gaccaccccg aagtcgaatc ggtgatccag cgggtcaagg ggttcgagga
   106021 ggagatcgcc aagtacccgg acatcacgat agtggcgaag ctgtcggggc acggcgtcaa
   106081 agaccaggcc ttccgcacgg ccgaggacat tctgcaggcc catccggacc tggcggggtt
   106141 tttcggcatc aacgacgatt cggcgctcgg cgcgctggcg gcgatcgaga aagccggcaa
   106201 gctcggccgc gtgaaaatcg tcgggttcga cgccgtccct gaggcgcgcc aggcgatcga
   106261 ggcgggaaag atccacgccg acgtgatcca gcagccgcgc aagatcgggc agcgcacggt
   106321 cgaggcggtg caaacctacc tctccggcgg cgccgtcgag ccgcagatat tgataccgtg
   106381 cgacctgttt gccaagcccg cggcgcagtg accggacggc cgcggccgag atatggattc
   106441 ctcctctgag acgcccgccc ccggcgggca cagcctgctg gagatgtcgg ggatcgagaa
   106501 atcgttcccc ggcgttcggg ctttgcgggc ggcgagtttc gccctgcgcg cgggggagat
   106561 ccacgcgctg gtcggggaga acggcgccgg caagagcacg ctcatcaaga tcctcaccgg
   106621 ggtgcaccgg cgggacggcg gggagatccg gctgctcggg cagccggcgg agtttcggcg
   106681 gccgctcgag gcgcagcggg cgggcatcgc caccatttat caggaattca ctctcgtgcc
   106741 atcgctctcg gtggcggcga acatttttct cggccacgaa gcggcccgcc gcggtctgat
   106801 cgaccacggc cgcgagcggc gcgcggccgc cgcccttctc gaccgcctgg gcgcagccct
   106861 ggatccctcg gcgctcgtct ccggcctcac ggtggcccag cagcagattg tcgaagtggc
   106921 gcgggcgctg gcccgcgagg ctcgcatcct cgtgatggac gagccgaccg ccgccctcgc
   106981 ccctcacgag gtcgcgcacc tcttcgccat ccttcgcgac ctggccgggc gggggatggg
   107041 gattgtcttc atcagccacc gcctcgacga ggtggcggcg atcgccgacc gcatcacggt
   107101 catgcgcgac ggggcgacga tcgcggtccg tcgggcaggg gagttctcgc gggcggagct
   107161 gatcgagctc atggtggggc ggccgctcga ccaggagtac ccgaaggcgc cggccccgat
   107221 cgggcccgta catttcgagg tgcgcggact gagcggcggg tttatcgagg atgtctcgtt
   107281 cgccgttcgg cggggggagg tgctcgggct ggcgggactg atgggtgcgg gccggaccga
   107341 ggtcgcccgg ctcattttcg gggccgaccg gaaacggcgc ggcgagatcc ggctgggcgg
   107401 ccggccgctg gaaatcgcct cgccgcgcgc ggcgatcggc gcgggagtat gtctactcac
   107461 cgaggaccgg cgccgccagg ggctcgtgct gggggcctcg gcccgggaca atttcgcgct
   107521 gcccaatctc ggttcctggt cgcgcctggc ctggatcgac cggcgccgcg aacaggagcg
   107581 gttcgccgag cggacggccg gtctcaacat ccggctttcc cacccggagc agcgggcgga
   107641 ggagctctcg ggcggcaacc agcaaaagct gctcgtggcc cgctggctgg agaccaattc
   107701 ggaagtggtc attttcgacg agccgacacg ggggatcgac gtcggcgcca aatacgaaat
   107761 gtacgtcctg atcggccggc tcacggcgct cggcaaatgc gtcatcgtga tctcctcgga
   107821 gcttccggaa ctcctcggga tctgcgaccg cattctcgtg ctgcggcggg ggcgggtggc
   107881 cggcgaggtg gccgaggcgg cccgcgccac gcaggaggac ctgatggcgc tggccgtgtg
   107941 aggccgcaga ggaaaggcgc gtggaaaatt cacggggcaa aaaacaccgc cgcgaggcgg
   108001 gcgcggcgcg gctgcggcag gcgttctccg actacggcat ggcgctcgtg ctggccgccc
   108061 tgtgcgcgct gttttcgctg ttgacgattt ccgagcagca cccgaccggc gcggcggccg
   108121 gcgacaacct gtgcgcccag ctcgccgccg ggacggcgcc ggggggtgcg gtggtggtga
   108181 tcggccaggc gaccggcgac gattcggcca tggtgcggac gctagtcggg cgactggcga
   108241 acgccggccg gacagtccgg ccgccggtgt tcggttcgcc cgcggaggtg cgggcgcgac
   108301 tcgaggcgct cgattcggcc ggccaggcgg ccccgctgat tgtgaccagc cacgcctact
   108361 atgccgccgt gacgatggtg gcggcgcagg tggcgggttt ccgcgcggcc aaagtgtgcg
   108421 aaccctcgcc ccaccgctgg ccgactttcc tgctggccga caatctccgc aacgtggcga
   108481 accagatctc ggtcatcgcc atcatcgcgg tcgggatgac gctggtgatc atcaccgccg
   108541 gcatcgacct ctcggtgggc agcctgatcg cgctctcggc ggttgtctcc gcctggctaa
   108601 tcggccgggc cgggggggac caggccgggc cggccgcgat gatcctggcg agcctcgggg
   108661 gcatactcct ggcggggggc gtcggggctt tcagcggttt catggtcacc ggttttcgca
   108721 ttccgccgtt cattgcgacg ctcgcgatga tgcaggtggc ggcgggtctc ggctacatca
   108781 tctcgcaagg caaaccgatc taccagcttc ccgattcctt tgtctggctc gggcgcgggg
   108841 tcgacccggt cttccacatc cccaacgccg tgatcctcat ggtcctgctc tacgccgccg
   108901 cccacgtggt gatgacgcgg acgacctacg gccgctatgt gtacgcggtc ggcgggaacc
   108961 cggaggcggc ccaccttgcg ggtatccgcg tcaaggcggt gctcttttcg gtctacgtga
   109021 tctgcgggct cctggcgggg ctcggcgggg tggtgatggc ctcgcagctt aagagcggcg
   109081 ccccgaccta tggactgacc tacgagttgt acgtgattgc cgcggtggtg gtgggcggga
   109141 cgagcctcat ggggggggag gggcggatcc tcggcacgct gatcggtgcc ttcattatcg
   109201 ccgtcatcca gaacggcatg aacctgatca acgtggaaag ctatacgcag aaggtcattc
   109261 tcgggctggt gattctcgcc gccgtgctcg ccgaccgtct caaacagcgg cgactcgcgc
   109321 gccgccggcc gggaccatcc gcccgccgcg acccgaaacc ggataacaca gcgactgcaa
   109381 atacggagca acgaccatga gcatcaagac ggcccttctc gctctcctcc tggcggccgt
   109441 ggggacaccg gcgggctcgc agccggaaca ggccgcgccc ccggagcagc gccccacgct
   109501 ccaggtgatc ggcaccgccc acctcgatac ccagtggcgg tggacgatcc ggaacagcat
   109561 cgacgagtat atccccgaca cctttcggcg gaatctcgcc ctgatggccc agtacccgca
   109621 gtacgtcttc agcttcgaag gggctttccg ctacatgctc atggaggagt actaccggga
   109681 ggacttcgag cgcatacggc cctatgtcca atcgggacag tggcgggtga cggggagctg
   109741 ggtcgatgct gtggatgtca acattccctc gttcgagtcg ctcgtccgcc agacgctcta
   109801 cggcaacggg tacttccgga aagagttcgg ccggaccagc cgggacatct tcctgccgga
   109861 ctgtttcggt ttcggctacg cccttccctc gattgcccgc cactgcggcc tccggagctt
   109921 ctcgacccag aagctggggt ggggttgctg ggtcggcatc ccctttgaca tcggggtctg
   109981 gcggggggtg gacggtgcga cgctgctgtg cggggtcaat ccgggcgagt acgtctcgcg
   110041 gattaccggc gacttaagcc gggacacgac ctggctcggc gccgcccggc ggcaggggga
   110101 gaaatcgggg ctcttcgcgg cctaccggta tttcggtacg ggcgacaccg gcgggtcgcc
   110161 ggactccgct tcggtcgacc gcctggtcgc ctcgatgcag agcgacggtc ctctgcgtgt
   110221 tgtcagcgcg ggcgccgatg acctggttga tctgctcgcc ggcgtcgaca ccgcgcggct
   110281 gccgcacttc gacggcgagc tggtgatgac gcgccacggg gccggctgct acacctcgca
   110341 ggccgctatg aagcggtgga accgccgcaa cgagctgctc gccgaggcgg cggagcgggc
   110401 cgcggtgatc gccgcgcggc ggggtgggag cttctacccg cgcggcctgc tgcgcgacac
   110461 ctggatccgc ttcctctggc accagttcca tgacgacctg accgggacca gcattcccga
   110521 ggcgtacgag ttctcgtgga acgacgaact cctctgccag aaccgattcg ccgcgctgct
   110581 caccggcgcc gtggaggcga ccgccggcgc tctggacacc cgcacccagg ggatccccct
   110641 ggtcgtcttc aacccgctgg gttttgaccg cgaagatgtc gtcgaagccg ccgtgcactt
   110701 cggcgaccgg aagacgcggt tcgttcgcgt cttcggtccg gacggccggg aggcgccggc
   110761 ggatgcggtc ccgatggccg gcgattcgct gcgccttcgt tttctcgccc gggtgccctc
   110821 ggtgggttac gccgtcttcg atgcgcggcc ggccgacgcc ccctgcccgc tggcgaccgg
   110881 cttgcgggtc tcgccccggg aactggagaa cgaacgctac cgggtggggt tgaatgagaa
   110941 gggagaggtg gcgtcgatct acgacaagaa gctcggtcgg gagctgctcg gggcgccggt
   111001 gcgctacgaa ctgctggagg acacgccgca caattggccg gcgtgggagg tggactacga
   111061 ggatgtcatg gctccggccc gcgacgaatg ggcgggcgat ccggtcatcg cggtggtcga
   111121 ggaaggatgc gcgcgggtgg cggtcagtat cgagcggcgc acggcgtctt ccacgctccg
   111181 cacgatcgtc agtctggcgg cgggggaggc cggggagcgg gtcgagttcc aggcggcgat
   111241 cgactggtat gagcgggagc ggatgctcaa gctcgccgtg tcgccggcga ccgccaacga
   111301 aaccgtgacc tacgacctgg ggctgggcgt gatcgaacgc ggccgcaatt ccgaaaagcg
   111361 ctacgaagtg ccggggcacc agtgggccga cctggccgcc cctgcgggcg actggggggt
   111421 ggcggtcctg aacgactgcc gctacggctg ggaccacccc gactcggcga ccgtgcgcct
   111481 caccctcgtg cgcaccccgg gcgtgggcaa aggctgggac tgggtgggcg atcagcgctc
   111541 ccaggatctc gggcgccacc tcctgggatt cgcccttcag ggacacgccg gcgactggcg
   111601 ctccggaggc gtcgtccggc agggggcgcg cttcaaccag ccgctgcagg ctttccgggc
   111661 gcagccgcac gaaggggagt tggggaagac ctactcgctg gtccgggcgg aggagactcc
   111721 ggcgcggggc ggcgcagcga ccgaagcgcc gggcgtgatg atcgccgcgg tgaagatggc
   111781 cgaggagtgc gacgagatca tcatccgcgt tcgggaaacc g
//
LOCUS       DAOPOE010000002        85224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_331_length_85224_cov_6.552055, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000002 DAOPOE010000000
VERSION     DAOPOE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 85224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85224
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_85224_cov_6.552055"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKM94_00405"
     CDS             complement(<1..850)
                     /locus_tag="PKM94_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65126.1"
                     /translation="MPLLRRRLLRSSLLLSAAILLPPGWDAAAVRAQRLFGNASLSWE
                     HLRQEALDVRADSLGGELVADTAVVGQSDNVTRESLLLSYEDELFTKNLLRLTANLQR
                     REYPFLEHAEYQPIYYLDLKSYGYTLTARYSPYTRRSQSIATGDAFDVYYRDWRATAA
                     VAYENLPTLAVVYSRVKNYDREEVPQFGGLSRNWVVESGYARGPLNVRANYNNLARRS
                     TLPGGISSTTETWSGTLGAGRSWPELAYLSATYNYYDTRRESAGPLAQDSRTHSASAL
                     VSVTALA"
     gene            complement(850..1992)
                     /locus_tag="PKM94_00410"
     CDS             complement(850..1992)
                     /locus_tag="PKM94_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD65127.1"
                     /translation="MTRAFRPTPRLRAALVAAAAGSLLAAAALRADNGHYRASPHGSE
                     ATGVWRLTELPRGSCAQCHTSHDPAAGNDYGLFEPNANRLCFAASAGGCHADRPTGAT
                     SGYPAQESDRLPLGSDDPGYFEFGSGGGRVPGVEHRVRWPGRIIWEDPRYSPHFADPD
                     MPIKDFYGDGSCANCHAVHGSGNLHDMLDTTYNGIVGSDVGLLPADYSLCLNCHSLDG
                     PVAMDDTARTIADYYDRSINPGRMSGHGVRSGGGYVPSGARLPCYDCHNPHGSEGYGG
                     LGGNHYLLSDQRPGWYGLTDIKTDNAQVRRFCLGCHPASDSPGGSASVEGLILSPLPS
                     NVPAHAAGDVTHCYDCHGRDYSSPTSHNVHNPAPGSAPGGPAPGDF"
     gene            complement(2010..2969)
                     /locus_tag="PKM94_00415"
     CDS             complement(2010..2969)
                     /locus_tag="PKM94_00415"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD65128.1"
                     /translation="MRVTVCAIAVLLPLAAAAGQWHTGESLMCQDCHLQHSSEQGEPV
                     AGGPYSVLLLKSSINELCLSCHDGSNPTAPDVQSPVQMYQGTPSGESAGGWFGLLGAD
                     NRGGHNLGIPTLAPLQTSAELVDLTCAHCHAVHGNSNYRNLRFDPADRGDSIVLVDGG
                     DVFSRFQPGDPPSAAATVAAYERDNIAYRQNSSAWCLACHDQARADNAGVPPAHFTGH
                     PSDIALDAYFTDRHTDPAHWTAGIGDGFPPAGGASAGIPRVPFEAPAADDYVSAREAA
                     ASNEVVCISCHRPHGGPFEYGLRWPYREGGEGHLAGCQQCHNQ"
     gene            complement(2978..3940)
                     /locus_tag="PKM94_00420"
     CDS             complement(2978..3940)
                     /locus_tag="PKM94_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65129.1"
                     /translation="MERKRIDTEAQGLAGSSSPRARCRRGPAIGAAALSAVFLFAAAV
                     SAQTLDGEIVIAADGLRLERPSSLAVSPSGEQIAVADPQANRIAVIDYFGRPLWSAGG
                     AVDLGRPLAVAFTGENELVFTRTADLRILRLIRDNPAELDSIADLAPVLEPHRRLDQL
                     LRTPERGWLALDRKTGEVLRFDESWGYQGIAVPHGSGKGKVLVPSALVPLADGRFVVT
                     DEKNYPAQLFSAEGKFLLYFGWSSPGEERGWEAAGGTADSRSLIWVADRTAAAFRIFD
                     PAGGAVAAVPFPQPLFRPAAMTTTIDNRVLVLDEFGRALFYRVQ"
     gene            complement(3961..7152)
                     /locus_tag="PKM94_00425"
     CDS             complement(3961..7152)
                     /locus_tag="PKM94_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD65130.1"
                     /translation="MKTFSRAAFLIVPLLLGLVLAPAASAVKTCYDCHQKQKQLYTSR
                     KFIHQPTKEENCESCHKRHGFAQTLVLTATTNDLCYSCHADLKAEFDKASVHFPVSDG
                     KCWDCHDPHASDKKGLLRAAPEGLDDPSACFMCHKAELDSSLHAAVPHPPFNELECTR
                     CHAPHASEHTGLLTAPANDLCGSCHSPADSRMTAAHGARHVAGLACTECHSGHAAGRK
                     GLLSDRTHEPFASGDCETCHSLPDDRGRVAFAEGVSPSDGCVTCHSDVAEKANLAHPH
                     AAMTGDNCTDCHRPHSSRFGSLLTAPQEELCGQCHSEVLTGKGLTPHLPAVLGQCSSC
                     HEVHGSANAALLARSEADLCLSCHSDFEAARDSAATVHAAADDCRQCHASHEGKTAAI
                     LRKDPSELCADCHAVDDAALGALSGHQPYLTSDCAACHSPHYSTAPHLVRGEGADACV
                     GCHADVQGRLGMTVPHPPAADDCRSCHVPHYGANAPHLLAAHEKDVCATCHDYDDLKV
                     TAAYVHAPARAGDCSGCHNPHGAMQENLVTGRMQRAVVDGRVVSQLPRLTGLSSDLCY
                     TCHDDLAEKFRRPGVHAPVAAGNCDACHAPHGSANPGFVTAPAPELCAACHAVDAGLQ
                     AAHDGFALDSANCLDCHNPHISEQPKLVRARLHPPFAEKSCDLCHAKGPNGEAALSGD
                     MASVCSACHEKVGESAGLAHQHPPFAAGECGACHGVHAADYDKMLKAEGGALCTGCHA
                     DVRTQMDLAVQHRPFADGQCLDCHRPHASAFTALKNKPAETFCLACHADLEQQIKNGQ
                     PHAPVVSGECTACHQPHAGATASLLTVSKENLCGSCHDASAGEMTAAHSGFAVAAADC
                     QNCHEAHAAPKGGGGLLMPRAHAPFTAGQCGSCHVGKQPARLIAPVKTLCLTCHEDFA
                     RTLDRPVVHAPVGDDNGCTGCHGPHVGYTGALQKKQGVAVCLTCHNTPEFSGKFKHQA
                     AFEDCRTCHEVHAGDYKRLLSTPDIMELCMTCHADAKETHYHPMGAGVIDPRTKQDLN
                     CVGCHSPHSSEYDKILLGDRDRKLCITCHSLSH"
     gene            complement(7170..7643)
                     /locus_tag="PKM94_00430"
     CDS             complement(7170..7643)
                     /locus_tag="PKM94_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65131.1"
                     /translation="MKLLRHTSALRPLLVGVLLALTALPAAAADVDSTKTVDHSGDVY
                     IIETRHRLFSDFLQVDTVGFDQPFFIGEEEWKARIILFNPHLGITTEGKGLQMSDTLY
                     NPAARIRVEQADTLVQESWAFHYVDAPHYRRTDLLGFKLLDFKVAAQYIPAPDKK"
     gene            7819..8529
                     /locus_tag="PKM94_00435"
     CDS             7819..8529
                     /locus_tag="PKM94_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOD65132.1"
                     /translation="MTSVEALINRQFMRWEREQLRRQEQEPPAAAVPPIITVSRQTGS
                     RGSYFASRLALALDYQRIHREVIDAVCASSGYCRRIVESLDERYRSNLELLIESALSG
                     TGIDRSDFTRQLYRVVLSIARLGGVVLIGRGANFILGPRHGFHVRFIAPRERRVRNLV
                     AYKGTTMIEASEMIDLSDRRRREFVRKIFGAEIDDPSYYDMIINADCIDIEELVEAVA
                     IAYRGKCSKLANLGNDIP"
     gene            complement(8713..8994)
                     /locus_tag="PKM94_00440"
     CDS             complement(8713..8994)
                     /locus_tag="PKM94_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD65133.1"
                     /translation="MKVSHDTHSNPMKWVIAVIIFVIVLTVAFDDVFGVWIPQSDNEE
                     SVGAVESPDVHGDAKVYSDEPPPQVPEPGTLILLGAGLAAMRLLRPKKA"
     gene            9264..9968
                     /locus_tag="PKM94_00445"
     CDS             9264..9968
                     /locus_tag="PKM94_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65134.1"
                     /translation="MDTTLGRATAHLAEPDDRRGAGDLIALINRNINPPAPVTADAAV
                     IRAMYVVSDEINSFGGCFPSDEHAGLCRLLVDSPVMVGHRRDRLPIARNFHAEEVVRD
                     GRPWVKCWFYWLRAADGAETLRENIDGGIYRECSISFTFLFPECSICGKDIRACTHQP
                     FARYADGDGEAVCHFRYRGIERVLETSLVYRGALPGTRLTKDLRDRHPVRIPDETLIE
                     APTELPAAERYLVAPA"
     gene            10011..10763
                     /locus_tag="PKM94_00450"
     CDS             10011..10763
                     /locus_tag="PKM94_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65135.1"
                     /translation="MRLRRLDNLDLPPALAGRWAADRLPPFDELGGYLIGRQGKTRCA
                     LAETECFLRGEPSPVTRLELRLLPGPDTPPLRAAGSGRFTVGLHRSEIGTLPQIDRLA
                     ARLATRAGVRIWPTTLSLREHAGYRYRPLLDRTAPGGGYTLRQPDATGTAHLVFADRD
                     AVHRFRLRQFQLQRLLHGAKFLADRCPATDDGAPGITTAEDHRRLSGPLLESVRDADA
                     FRLRLAGRLAGEYVLRPVTIDGERRHLFYRCT"
     gene            10823..11131
                     /locus_tag="PKM94_00455"
     CDS             10823..11131
                     /locus_tag="PKM94_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65136.1"
                     /translation="MPNAHGPGFNLLGPVINIVTIIMGVVTAYFVTIQSLKIDLAAKA
                     ERAVVETLDKRLTAFEVSLTEGAVTRDQFYEFARDTEARLARIELYLAQQSEDERGNK
                     "
     gene            11118..11501
                     /locus_tag="PKM94_00460"
     CDS             11118..11501
                     /locus_tag="PKM94_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65137.1"
                     /translation="METNETLIEQVDDLAAAWTPDNLCELDFDRIRDLLGRHRRLLAE
                     HESLREDLAALRQDYVARIGGMAKAIAAVTRRTGAAQAHDAAAAVLEYLRTLETMTAA
                     QLIEQYRLAGARFRDAFPTSFGRLP"
     gene            11644..12063
                     /locus_tag="PKM94_00465"
     CDS             11644..12063
                     /locus_tag="PKM94_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65138.1"
                     /translation="MTIRPQNLEAVAPVLATFAVHQTGGVDDLKDAHLGEPVMLTGSN
                     EAGPISNGGQLLGKLVALTLADGDDGSRLATVQIGGVCRLKISATYPAAGNRVVGGTA
                     GTVKQAPAVSGDPAGGNIARGTVLAVNGAVDCTFLLN"
     gene            12075..13178
                     /locus_tag="PKM94_00470"
     CDS             12075..13178
                     /locus_tag="PKM94_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65139.1"
                     /translation="MTFEQFLRSAALTGAPRECDREWAGGAVDLSRTAGIEITRALYG
                     EAEARGLSLSELLELDDYDPSPAGAPLDAFERQLALAGIRFGGKTPTTVEQFYQQAPA
                     LLPEFMRREIRAGQAMRPELRRLIANTAIIASNTYTPFFIDTTDAACFSLRPVAEGAE
                     TPQVLVTEQNHAVRIADYGLALKASYKALRYRTASQFRVLLWYIGFRLQTDKIGMLVE
                     TILNGDGNDNAAAVITSAASGVLAYDDLVNLWSQFAPFEMNTIICGVNLMKEILTLDE
                     FKDPMAGYRFQNTGDLFSPLGASLVRCDELAADLIVGLDSRFAIEEVVTQPLTIEYDK
                     IIEQRFEEAVISESVAYAKVIHGAAVVLDTVHA"
     gene            13316..14728
                     /locus_tag="PKM94_00475"
     CDS             13316..14728
                     /locus_tag="PKM94_00475"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOD65140.1"
                     /translation="MRQTICPSTALHPLGAPPGRKLLRVSSGPHAGREAVLFQAAPGE
                     IACAWADRPYAGWSPPAAVTAQAEDSPFDAAIMPDGTIHLVYTEAGTGYLAARKLTLT
                     GGAWVPGPPVTVVDRPIARYPSLAILPDGALWVCWTGIESGTHRLFAKSSSDGGTTWG
                     AGPSDPGESLTLGASSAFGRLAVGPSDLYAAYTNGGMQLCVRSRPLGGGAWGTSTNLA
                     AGENFDEHFDVAAGVDGRLGVVWDNGALRYREFDGDQWAPTVVLDGAGGSWPQLVFID
                     FAPLVVYLSESGAGQAMPLYTTRQGGAFVAPAALDGRAAPFAAVALYDTVSASWANLT
                     AEATGTAPADIAHPVSGALLAHAGDALYLGMEERFRFAKVRLSSAGAGGSTVCSYWDG
                     SAWAAITPASGPYNFDVLEHDLLLWNDYNTVPVDWQKKPVAGAVHFWVKIEALADFAA
                     APVGSQITAVSRVTALTVRR"
     gene            14732..15544
                     /locus_tag="PKM94_00480"
     CDS             14732..15544
                     /locus_tag="PKM94_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65141.1"
                     /translation="MSYTTDELVRRHLADTRPLADRVTDQAIVLGESGWVLFWRGPIE
                     AGTVVVKALRGAAPLRRALALAGGSAALGVAPVVPGSVVAASDSSLGTIYAENVDFVI
                     DYPRAVFYAKAGGALADDASVTVWLREYTLCAPGLDYALDAEAGALRRLAGGSIAPVE
                     TVRLDYTPLYAAGDDMLIAAAVAEANGLVGAAVDPARAFGADPALEAAATCHALAVVC
                     RGSAARVLATRPEQERAAAAWTRLSELYADRADRLLAAFHPPAAGPAAPSAV"
     gene            15556..15930
                     /locus_tag="PKM94_00485"
     CDS             15556..15930
                     /locus_tag="PKM94_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65142.1"
                     /translation="MLTPLNDVLARRLWFIPGFVVWGVPGLSEIDFVAAEQIGGATAL
                     RHGAAVPGGGPQTVLYADLVDHRGNALPAALASPRVWLRPQGATPAFVVGAETPSSFR
                     VARDPDGAGPVTADLFVIEMGD"
     gene            15932..16273
                     /locus_tag="PKM94_00490"
     CDS             15932..16273
                     /locus_tag="PKM94_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65143.1"
                     /translation="MAPGAQIRERLLCEWLARLELLIAGHFDALLGFARPKSRSRSGG
                     ASGPPRSAQNAKVSELIKMIQLYARLADSRPGTDEFWEMIERIRQEKLPLRPTGQLPA
                     AEASPKKGRRP"
     gene            16270..17019
                     /locus_tag="PKM94_00495"
     CDS             16270..17019
                     /locus_tag="PKM94_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65144.1"
                     /translation="MTRSAAVLLAALTPLLAEAARIDTLRGPSQIEDATIYGYLTCDG
                     EISGEDCRRYNTGNCLYFSVGTQGTGNHRAVLRFPGWDGVVPDSSVLELFCELEDDGA
                     DRRIFLYPLTRRFVEGVEGFGAGGIPADSGVTWNHAWLAHGRGDSLSWTSPGGDFTAA
                     VACTLIAADSGKYQRVAGFNRMLAYWDSTGSDPGVILIGENVFPANTAKKTFTSAEGA
                     GDRAPRLLLYYPGAPVAAGRRRLQSTAAPAR"
     gene            17046..18125
                     /locus_tag="PKM94_00500"
     CDS             17046..18125
                     /locus_tag="PKM94_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65145.1"
                     /translation="MNYRLAFILALLLPGMSAAARIVHNRTTLATGEDSLAVAFQVLD
                     SLGNPTEADSIFISVIGPSGGMTAVDSMTPADARVSVTAPGGQPAYVFHCQVSAIDGA
                     GAPGVYALTVMAKSTALGLATQTAAAFQIVAAPLSERLALLDDTVRVNGGVIDSNRTE
                     RGMAIGSGAVSVVLIAYDSTRTQVIPGVRVVIRDLAQTLLHGLGFTDVSGAAAFNLDA
                     DSVLAVAAAPGYLFPPFDTLAIVGATMDTLFGTASYPEPPPAGGLCRVWGCLYGIDGS
                     PQAGATISAMLPSGVARFGGAVVAPSPVSTATDSTGLFQLDLIPSELLESEDPRYDLT
                     IFRPDGVILRQRIAVPNQPLWQLEW"
     gene            complement(18184..19458)
                     /locus_tag="PKM94_00505"
     CDS             complement(18184..19458)
                     /locus_tag="PKM94_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD65146.1"
                     /translation="MANHKDRSDRRRLRLGVVGGGAAGLFAAIAAAETGRPGEVIVWE
                     ATGAPLDKVRLSGGGRCNVTHRCFEPADLVKNYPRGERELRGPFARFQPRDMIAWLEG
                     RGVRLKAEADGRVFPASDDSGAVVACLLREAERAGVRIRLGSRVREAAPAGRDTDDAP
                     LFDVIAGDGSREQCDRLLIAAGGGRPGHALAQALGHTIVPPVPSLFTFTVPDLRLQGL
                     AGVSFESVQLRLAVGGERFEQSGPMLITHWGLSGPAVIRLSAWAARPLHESRYRAELV
                     VNFVPGSTAEQVYRGMTAHKQAHGKRLAHGDSPVSLPSRYWSRIAAAVGIPPETAWAA
                     VTREHMAVLAEQVTETHLAVAGRGIFKEEFVTCGGVALGEVDFRTMESRLRQGLYFAG
                     EVLDIDGLTGGFNLQSAWTTGWIAGRSMAGVK"
     gene            19554..20063
                     /locus_tag="PKM94_00510"
     CDS             19554..20063
                     /locus_tag="PKM94_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOD65147.1"
                     /translation="MTAQESAPAPRTQPRRRAVDILGLLGWLILVFAVSFAASRYMPG
                     EWYARLHKPSWTPPNWMFPVVWTLLYAAMAAAAWLVWRRDGFAGARAALVLFLAQLAL
                     NGVWTYLFFGRQWVGLALVDILALLAAVIITMVLFRKHDRRAGYLLAPYIVWLAYAST
                     LNLGVLVLN"
     gene            complement(20147..22414)
                     /locus_tag="PKM94_00515"
     CDS             complement(20147..22414)
                     /locus_tag="PKM94_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65148.1"
                     /translation="MHALTHTCIMRGFFAGFLLAASAAGPAFSEVMSETSDHVTFALG
                     DGLAYVTGTVSDSAAQVATVSLGIGFDSLFEQLNGAITEIHVKVLYDTTRMSFLAAEA
                     DPGWYAYFDAQESPHGVVQMQMTLDTTSPLHTASAGTREYGRLRFIHECWPQDSLPGG
                     VNFSRNGMDNSVTVNGIIYSPGSEGWDDGSVDRMPDSAYHITLEIADTAVTGEVGEEF
                     VLTEYATTDFRLSLVWQAYTFDTAKLEFRGFEMPPYFGGTVTLRSDTIVITALNFSGV
                     DEGHHFPLVHLTLAPRCPLGNTSTVLNAVPGWLTVWVSECSGSIDPAAVSDPSVTIAM
                     ADTAAYRARYLGEPVTMQDDGQLLKFGVLMRSTFAAGMGSQSDTGLTMNIDWTPNLDF
                     AGIDHYAPAVKFLDLSQGDLTAVYQTWDAARANYLPGGGDFDSLVTLKVRFDTAGFTP
                     SWNNRYLRPKFIHDFGIGHGRTVVPDTVGCLRADSVNGILDAWSGGAFDSVEVLMGQL
                     LAGGGSSSTACGEVDFYVRGTCAIDSFSVRITVPTWACIYGIIDRMTGVIPTFVNYHT
                     YDIQTNSLFAGVGPTGDPVKVCRLRIGLKSPCLRGKPYGIGISVTNGKVWDYAAGGPM
                     YAATNGSTITLKCSSTGTGCSSCGSGNVDDDIPDDPNAKVEPTAPSTFALHQNFPNPF
                     NPATTIQFDLPVAAEWRIEIFNTLGQLIREFHGASAPGSVTVEWDASAYASGVYLYRA
                     TAADFVETRKMLLLK"
     gene            complement(22772..24976)
                     /locus_tag="PKM94_00520"
     CDS             complement(22772..24976)
                     /locus_tag="PKM94_00520"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOD65149.1"
                     /translation="MLPASDFTPVYRPSITLRPAAGRIEVDGDLGDEGWRNAARTDRF
                     VERSPGDMTPPAVRTEVLVTYDKANLYVGFICDDDPRALRATMTRRDAYSGDDCAIVL
                     IDTYGNAASAYEFFVNPYGVQKDILWTPTVGEDASFDLVWRSAAQVTPEGYRVEIAIP
                     FSGLRFPQGAEQSWRVDFWRNRPRASSFQYSWAAYNRSEQCWPCQWGTVEGIADVAPG
                     KGVELLATAVANQSGQIVAAEGYPFDNEKVEGEMSLAGKYAVTSGMTVEATWNPDFSQ
                     IESDAAQIDINSTIDLLYPERRPFFQEGADIFQTLFNSFSSRTIWDPRYAAKLTGRAG
                     RWRLGFLSARDENSPYTVPLEESGGSLRAGASTVNVLRGSLSFGQDSRGGFILTDRRY
                     DWGGYGTVASLDADIRLSRNYSWVGQVVASYTGEPDRPELFGQPERFEGMTFDNGKHT
                     VLTDGESFSGNALITQFRRQGRHLNFRLNYDQVDPTYRTQTGYDPWNDYRNAFVNAYY
                     LFYGEGRLIERLAPNIYVDRRWNYDGRIKWTHGTAGLEANFRFWQSYASLTYTAGDEN
                     WGGVQFDGLYAVRIGAGGVISNALAFNGDLSFGRDVAVRQSATGNQVTGGIEVDLKPL
                     DRLMIEPSYTFTRSRHVATDELLYEQHITRTRVQFQATRELSLRLVVQYNDGSERWDI
                     DPLLSYQLSPFSVFYVGTTYDYQNLAAGTDEPDQWRMSSRRFFLKLQYLFQT"
     gene            complement(25561..26157)
                     /locus_tag="PKM94_00525"
     CDS             complement(25561..26157)
                     /locus_tag="PKM94_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65150.1"
                     /translation="MHITRLLTLSALGILLLASLGAAEEPQKPAAKPDNFGILDTVYA
                     EVSRIDPQNWSITISATNDENVEGLAVPLKLSSGTVRIVADSAVYTGGRVDHFAYRGF
                     RPDTMQQCVTLGMVANMGPSTNVLAPGKGRLVTVFVSSIEDQPVENLVVDTTTTSPSN
                     SLMLVAQFEEIKNRLGEEAIRADKRLEIYPAFVVVKSK"
     gene            complement(26219..27703)
                     /locus_tag="PKM94_00530"
     CDS             complement(26219..27703)
                     /locus_tag="PKM94_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65151.1"
                     /translation="MGRISRILILAAGFAAAFGCADNPALRLRFQAEKKLFDAEKIIA
                     QAQVNPREAPPELVRRAGEKLSAAAEFALAALDSVSEQANPVEYRELQQIAFQAGNEL
                     NRLLYNQRRYEESIAVLTRLLSQSALAREELMVTQVNLGRTLQASGRWDSALAMYTSA
                     LETFFPPVDSRGEIVKPLFDLPAHIFAVARQVGDSVEAAYHLERAVSYYTDLAAKTSD
                     RLLQASCRAALAGLYRETGQGQKEIAQLEQLSDTASQAYPAVRARIAEIVGFQFRDFT
                     RAAAMYDTLLARLGPSDTAYYPDLLFRKALIRMEQKRYGDARQILVALKRDHPRYWAM
                     TPPAQLAMARTFELEDNWDRAEVEYHALIEGYRGSDEAMGAFLYVARRYEQLGRKDQA
                     DRWYRDAREYYAQVARYSQEGLMEARALSFQAELDSRLGNWAEAAGTLEDLYRKYGRH
                     EIGRQALVRAAAIRRERLGQQQVADSLLTALKAGLTEIEPTSEL"
     gene            complement(27874..28632)
                     /locus_tag="PKM94_00535"
     CDS             complement(27874..28632)
                     /locus_tag="PKM94_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD65152.1"
                     /translation="MRILVLVLCIGFCLATPASAFNFHYYDFGDSPFDENNNWSPINY
                     PGGIGNLPSPGTLGEGGEEFDLEGLKVAVDQDYVYIALTNSFGYSAYSTGWGRNYNLG
                     DLFIGTNGGGYQYAIDLVNGGSNGLYRVTGRTGIPNIPGSYYGTPIAPLAGDYRMTSG
                     TYLGQVNTMLSFWDDYELNHLMPGDGDTYVWEFRFARSLLGDFQSLDFHVTLGCGNDL
                     MEKSVAMVPEPATVLLLGLGLSGVMLRRLRRRDA"
     gene            complement(28908..29255)
                     /locus_tag="PKM94_00540"
     CDS             complement(28908..29255)
                     /locus_tag="PKM94_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD65153.1"
                     /translation="MKAIIVLLAVTLVCAAQAVALPLSAPRLVDVSSIDWGAQITVAD
                     GSVGTTGQAYFTGDPRTSGSQATGTDGTGGVIIRPGNTTLPKTVTTVPEPATLVLVGL
                     GIAGLALLRRRKA"
     gene            29576..30817
                     /locus_tag="PKM94_00545"
     CDS             29576..30817
                     /locus_tag="PKM94_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65154.1"
                     /translation="MCASRRNSGQLAAGVLVPPPSALSTGGQIDRQLRLSTSDLQRGR
                     YRILLYRFLADSIPAVAACVGTWVRLAAAPGRFLVDGDVSPSVRRQVEERLERLSAHV
                     YRSVGGTRAGGADLPVDIAAGLFRDGFFGGFVTVDATGTGVDRFIPVDAARVNLTVED
                     GRRQFRYAADHGDLDLDRPDFYYIPLSGGVNAPLGRSVLQAVPFVAYIEQQLVDDMRR
                     SSHNAGFHRLHVKVTPPERLAGESDTAYVQRINGYFDETVGMIRSCAIDENPVTWDNV
                     AIDYIGPDATRSVTNSWFFSHRSMIEEICAGTGLAPFLLGYSYGATTSWSGFKFDLVM
                     RQVRSVQAEMRRLIEWIAAIDLALAGFSARVRWEFDNTFAYQAGERAGVESTRINDLI
                     RLYEVGLIDRATAAAKAAGLV"
     gene            30887..32239
                     /locus_tag="PKM94_00550"
     CDS             30887..32239
                     /locus_tag="PKM94_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HOD65155.1"
                     /translation="MNALATDCESIDWRRALEDPAYFAQAVLGVTLHAGQRQWLENAL
                     ARENVLVTGNRWGKSFVSAVKLLRHALFRIRGSLAFDGSGRYRAVAASITQDQANLLF
                     NQVVRLARQSPVLRPRIASLVRSPYPMLTLGNGAVIEARSTQNRGEYLLGNDYDLFVF
                     DEVAFEADPEYVVEEVLLMRLADREGMLDLVSTPNGKNWFYRRVRELAQGARPGYFQS
                     GDSRANPYISRAFLDERVRHFTARRLAQNIEGQFVDSGGEVIRGSDIDAALAGISGSE
                     EAGGAASGEIRYLSGWDLARKRTATVGITVAAADGPARVVALERLKETDWATVIARIR
                     ARQEQYPGQLVIDATGLGDPIVEALREYRPTAVIFTGPRKAELLTNVELFHARRRIVY
                     ERWELPDGPGRTWSLEDELRRARWDDNCECDSLMALALALWPLRTRPAPVIAPRLGRA
                     "
     gene            complement(32260..33501)
                     /locus_tag="PKM94_00555"
     CDS             complement(32260..33501)
                     /locus_tag="PKM94_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD65156.1"
                     /translation="MLTTFSHSALQTFAQCRRKFQFRYLERPEIPKRISAEAYLGTAV
                     HTVLQALYERGADGVELSLEAALEIYERAWREVDRALLAVVTEHMGIDDYIRIGREIL
                     ARHHERYRPFRPGALLGAELHLHFTLPGTPYRLSARIDRLWRRDDGTVEICDYKTGKN
                     LSRPHDPDYFHQMGLYQLAVMANYPQYRDIEAAQYCLRMDEVVAHRFRPDDLELLVEN
                     IRQQILAIHEADRLSQFPTRESSLCNWCEYAALCPAKRHKLLIEAGEADRDGALRRAY
                     AKATEYIARYAAVKELEAEMAALRADLVRLAGELQMTKFSGEGGEVAVGITEEEKFVT
                     KTDSSTDFASLTALARDAGLIEYFKLDSAALMKDGYAKGLLPAALSAELKKFIRRKQT
                     VTLRVKLARAGEDPDAPSGSL"
     gene            complement(33538..34578)
                     /locus_tag="PKM94_00560"
     CDS             complement(33538..34578)
                     /locus_tag="PKM94_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD65157.1"
                     /translation="MPESKPSKFDLAVIMPVRNEEKYVGQTLEQLYLQDFPMDRLEVV
                     VADGGSTDRTREIVESYKTRFGALRLLDNPVRRPSSGRNIGIRNSTAPRIVVIDGHVY
                     IPSKTLLRDVVETFDRTGADCLCRPQPLTPPDLNDLQTAIALCRGSALGHKPGSEIYA
                     DYEGEVDPTSSGAMWTRATFDRVGLFDENFDAGEDIELNFRVHRAKLKAWLNPALRVY
                     YYPRATLPALWRQMVRYGLGRFRMSRKHGLWAPTHWLAAAGVVLCIVLFVLGFLSLAV
                     MKLFQIVAALYLLTVIFYSLSLALRHRNLNCLLYGPLIFPTIHFGLGVGFLKGVIESY
                     SPPPAREAGPPQ"
     gene            complement(34718..36151)
                     /locus_tag="PKM94_00565"
     CDS             complement(34718..36151)
                     /locus_tag="PKM94_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOD65158.1"
                     /translation="MTETSPETRVQRPKPKLVRVVITRDNMSAMMAVSDPEHLGGEVT
                     LDDVREEIVRAGVTHGIDWDAIKRVLDQRLYDVPTKIATGTPPRRGKDAAFEYAFEPE
                     TQHTPEESGDGRIDYRSINFIQNVREGTVLVRKIPPGDGEDGCGVDGRLIPGIRGRDF
                     NFRQGKNTRVEPDGLSLVAAASGAIVFTRGTVSVNDVTRIEGDVDMSIGNINCIGSVR
                     VVGDVRPGFTLNVGGNLEVGGNVADCTIRCEGNILIKGGAYGKGEGMIKADGDVLVKY
                     AEGVNIISGNDVLVGDELLNCQVTAAERIIVRARKGKIIGGESNAGREIRVATAGNDA
                     GTATILRVGYDAELMREYRCTGAEIARIEKDLARIRATLLTMYRLQAEGLLDPQRAEA
                     LGRLEEFVAAAPGELERLADVRYRLEERLREVAGAQIIVQDRLYHGVSAHFGVIYREF
                     TDIHKRCKVTLEEGKVVISEYRPDRDG"
     gene            complement(36162..36557)
                     /locus_tag="PKM94_00570"
     CDS             complement(36162..36557)
                     /locus_tag="PKM94_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65159.1"
                     /translation="MKSAVSILVVDDEAMMRLLLEKILSREGYRVATAPDGETAAALL
                     RSQPCDIVISDMKMPGMNGFDLLKIIKREHPRTGVIIMTAYGDTYTVKDALLLGADEY
                     ITKPFKSYEIALVVERAYWRILAASNSSA"
     gene            complement(36621..37037)
                     /locus_tag="PKM94_00575"
     CDS             complement(36621..37037)
                     /locus_tag="PKM94_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65160.1"
                     /translation="MLDSDMQKLTRLAQERGEPFRVLIVDDEKWVRDVFRDFCGTTDA
                     FRVDLADSGLEALAKVEATPYDLITVDLIMPEMSGLEVLSEIKKRRPRTPVVVITGNA
                     TDKLVHKAGLLGACRVMYKPVQLEDFISEISSALSR"
     gene            complement(37147..37521)
                     /locus_tag="PKM94_00580"
     CDS             complement(37147..37521)
                     /locus_tag="PKM94_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65161.1"
                     /translation="MTKSRTDEFRPIDPAIIERVDELNEEVKVLALNLAIYLARAKGR
                     SATLSRLEPDFIKLVNGTVKVVHELADIINAARNLEAMVNDLATRPPGSDPIEGRLRA
                     ILEQCTRIMDALAEEGRVDTKG"
     gene            complement(37518..39452)
                     /locus_tag="PKM94_00585"
     CDS             complement(37518..39452)
                     /locus_tag="PKM94_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOD65162.1"
                     /translation="MKTTSDIIEREQPGEEVHIRKRTVEPIAAAAPLVVTEPPDAEVL
                     REALRRGRPLTFPRPAGETPTEGRDPARGKAVLLALDHLDIGLFLVNRQGYITDCNEA
                     GSELLGIDHTTPWENRHVSNLTAVFPGDVYDQFDRLLSGDRACVRREIAISGPGARPL
                     AVTLCAVPLPDQAAEIVGIVREASADQNRRAHDEFSILAEVAAALSSSLELRQILRII
                     LTGATASQGLGFNRAFLFLFDRDRHELTAHMAVGPTSAEEAGRIWTRLAGMHLSLAQL
                     LDVHPSGSEDPTDRLFECIRGLTIALDNESLLAHICDSGTGVNLEPDAELDDLTAGLL
                     RRTGTTRAALVPLMSKGTCQGLVVADNAITGAPIGDDAVRLLKVLADQAAVAIQRARL
                     YDRERQRARELERTYRRLEQSQEQVIRAEKMSVMGELTAAVADELRNPLTIIEGFTNL
                     LRESHRSECGAEYLNIIASEVRRADALLNQVIEFASASSRQNQVFDFSVLAANVIARL
                     CGQTEHRPSGLSLSAATERLSVYGNPDQLGNAFYQLFQMIVEETADEARIDVRTERAD
                     GRARLSLAFQTRPERTERLLRMLDQIFARQTAALRLQVLVASETIRYHGGDYRLSTTD
                     NGAPLLVLEMPLAGQQVGMP"
     gene            complement(39460..40080)
                     /locus_tag="PKM94_00590"
     CDS             complement(39460..40080)
                     /locus_tag="PKM94_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65163.1"
                     /translation="MRGRALGLLSAAMAAGLLLVSAGPGETPGRTVALVVAPPDLPWA
                     DCEIEARLVQRLTRHDDLRILTVRAETPPHPAFPPDHGDAAALAAWGQEAGAAYVVIV
                     RVESERLEHRKSMQIPLVAHRYVALGVIEGELRVIDVARGRLALAEPFHVQKKGPQVF
                     QGSVDNDIHDPDLHISAPDKSVFFRRLEEELAGRLVRRIGTVIRMR"
     gene            complement(40077..40598)
                     /locus_tag="PKM94_00595"
     CDS             complement(40077..40598)
                     /locus_tag="PKM94_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD65164.1"
                     /translation="MKRVTGTRFVHDVEATDPGVGARRPLRIEQENRRRYVRLEIVSP
                     MWLRRLKDAFGNFTPTEAEPELSGEILNIGAGGVLVELAQPLNEGDIVAMRFRLEGLE
                     SLDNVLGLVKRCDHDGAVCLTGIEFVTREHLRDRLSAAELDLLGERAADFNEGVRRVL
                     ARYLYTEQRKSPA"
     gene            complement(40612..41376)
                     /locus_tag="PKM94_00600"
     CDS             complement(40612..41376)
                     /locus_tag="PKM94_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65165.1"
                     /translation="MTVRETAGDQALDALEIERFDLSTLVGRKVTLYAEQFPGKPLTS
                     RVTVAAGKAITIDRSGHAGLIANLVNNQRVVLQIVYKGEPVSVSAVLRRTEGGGCRIY
                     LGDTVTPLRRRRFCRVPIACPFRLAALPIARLAPTGLSRLRWLETETVNLSGGGALIV
                     FTSVLEPRTYLFVNVGGPELGFPSLLLGQVRHSSAVETGRWHVGLQFIAREDRNGHLP
                     FPTVRPMPGSVFALDQRMQREIERKLIAWMHEQNAV"
     gene            complement(41373..42119)
                     /locus_tag="PKM94_00605"
     CDS             complement(41373..42119)
                     /locus_tag="PKM94_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HOD65166.1"
                     /translation="MTPPATTSASGKINLWDRLEIVIGDGPEAGRYITRLEDFGQGIL
                     VIGAPEFVGGTDLIRNGAPCRVIFTRDDAVYRFDSQIAVIARREKRIYLLRAPEAIQR
                     VQRRQFVRLDLRRPLTVTVLGQPGSPSLPPHAVHRTVSLNLSGGGMLIEAPFELRPGA
                     RLLADVEFFPAIGIPTPVAAVVRRVDRPAAGGLAGIEFVRADYLAQVFTADELGRLPE
                     SARQFSRSMQNKLVNYVFQEQVKLRQKGLL"
     gene            complement(42135..43319)
                     /locus_tag="PKM94_00610"
     CDS             complement(42135..43319)
                     /locus_tag="PKM94_00610"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOD65167.1"
                     /translation="MASDSPFFLSKVECPICRTINEFETVRMGAYVEEGRDSDFCPRD
                     IKWRYPKYDGYNPLVFFVATCGNCYYSREYTPAYKEWKKDNAFRTYRLKATKDKHLDQ
                     LATADSIVKRLGKAIDMARFPNQTAILKLHLAAFDEQLFEHYSRLDLGRFYLRIGWVF
                     RSLEGGENPQISRMRGFLYELDTRHDGITDALGSARDRVAEFLESVQSQFEADTLTSE
                     LKAQILPYRERFLAEGAALTERITGVEQRLADLTTLIEEFKTVGVGLGDINSEPTFAG
                     FPTFAEFLYDLKKSWDGIVTSEREALQKAVHFYRQAFTDGRSIAPGNQQIQASYLIAE
                     LSRRTGDFATAREFFNHTIRHGQEFIYRNRHDQTQTALARKILELAIEYGKSSMEAPQ
                     GA"
     gene            complement(43341..43721)
                     /locus_tag="PKM94_00615"
     CDS             complement(43341..43721)
                     /locus_tag="PKM94_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65168.1"
                     /translation="MPLTNDMWIDLALTATGYLAAGALSMVMYSMFRRKTRPGAAEAP
                     SPAAAPAPAPPLAADRPRDDVRYVGFGERAAAGEPAASLRRNRAEVLRLARGMLRTGA
                     TDAEIKAVLPVSDAELAVLNYERS"
     gene            complement(43740..44069)
                     /locus_tag="PKM94_00620"
     CDS             complement(43740..44069)
                     /locus_tag="PKM94_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65169.1"
                     /translation="MPPSIETIDKLPPPPPHDRVNETAKANEEQDREAFKRALKRRMA
                     GGDRRADDGPDDPLIVDVDLSKQESPPDQENRDDDPTEPHQAQDRAADADDREEPPAP
                     LHIDLQA"
     gene            complement(44101..44976)
                     /locus_tag="PKM94_00625"
     CDS             complement(44101..44976)
                     /locus_tag="PKM94_00625"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOD65170.1"
                     /translation="MVSTAKVEPKKRGCKPRRVSVAETITPEITPKKTRKWDVTARDW
                     AQYSKSRDPEARQRLLAKYIPLVRNVASRMAMGFPRSVELPDLVNTGVIGLIEAFKNF
                     DPGRGVKFETYAVPRIRGAILDELRALDWVPRSTRAKSREIERALVDLENELGRPPEK
                     NELAKHMKISERELHLAIEDVSCTQILSLDEVIYREDDNRQVPRIETIQDSTRATVLG
                     EIERGELRSFLEVAMDRLTEQERLVISLYYHEELTLKEIGAVMVISESRVSQIHTRAV
                     SKLRSMVRERFALTG"
     gene            complement(44984..45853)
                     /locus_tag="PKM94_00630"
     CDS             complement(44984..45853)
                     /locus_tag="PKM94_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOD65171.1"
                     /translation="MTRLHFVAPASAVPQGGPRLVSVLSGKGGVGKSILAFNLAERLA
                     ALGLRVLAVDGDVYGGNLHILANRACAVGIGEFIDRRLSLAECRTPLAERLDLLGVSP
                     RLCDPEAWSAGTVGSMLKRLRAEGAAYDVIVCDHSSGRSRIATLIAHGSEINLLVVVP
                     ELTSISDCYGLFKHIRQARADIACRLVINRAADAEEAQYLHQKFGAVTERFLGEGLRC
                     LGFVPEDAAVRTAVAAQQPLAAAAEHSLAVQALTRMARSLVRELTPEGADRCGLFTLA
                     DDKVNPAEADIRK"
     gene            complement(45846..47003)
                     /locus_tag="PKM94_00635"
     CDS             complement(45846..47003)
                     /locus_tag="PKM94_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65172.1"
                     /translation="MIIKSFTAESASAAMKQARREMGGDAIVLKTRQLTDRYNRPQVE
                     ITACLEKPTVGRMSDILDSRPASVNRLPVASDGEPAPAAVEDAGPEPLADEPGAEAPV
                     PSEDRLARIEATLDRLVCLSATRGGEEIADPAIRAVAASLRDADVPEDFIDAFLLARP
                     ERFLGPGALAKIRSELVKELSRLMVPDLKFRPGDRVLFVGPAGAGKSSVMGKLAAHLA
                     AVEKRKVALAGVDFQKVGAHEELAGYADLLNLDVAEVAGEHADKAGTKDSITLIDAPA
                     LPAEPDRREALVRAAAKIGARYRFLVIPATMRSADAIDLAARYRWFGPTHLAATMLDV
                     TRRQGVFIAAARAAKVKMVFFSDSPGGLGRLKAADPAALAATLTGREAADD"
     gene            complement(46993..49080)
                     /gene="flhA"
                     /locus_tag="PKM94_00640"
     CDS             complement(46993..49080)
                     /gene="flhA"
                     /locus_tag="PKM94_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOD65173.1"
                     /translation="MKAGKDHTGLLDLLVSRSDIALAIGVIGIIGVLVIPIPTPLLDF
                     ALAFNITFSLVVLLTTLYVTRPLDMSVFPGMLLIVTLMRLALNVASTRLILGQAFAGD
                     VIQTFGNFVVQGNYVVGFIVFVILVIIQFVVITKGAGRISEVAARFTLDAMPGKQMAI
                     DADLNAGIITEEEARARRRDIAREADFYGAMDGASKFVRGDAIAGILITLINIIGGFI
                     IGIAINGMDINEALRTYSLLSIGDGLVTQIPALLVSTASGIIVTRAAADSNMGTDLTR
                     QLTRQPRAVMVAAVVLLIFGFLPGMPTFTFLALGGVVGGLGYLTTQLQAKRAVEEEEA
                     KKRSRAQEHRTQERTEDLLKVDTLGLEIGYGLIPLVDPNQRGDLLDRIGSIRKQLAAE
                     LGIVVPPIRIRDNIRLRPNEYQIKVKGLRVAGFELMTDHLLAINPGYVREKLEGFDTK
                     DPAFGLAVTWILPNLREVAEAREYTVVEPSAVVATHLIETIRRAAPEILSRQDVQHLV
                     DTLKEDYPALVESTVPEIVPLGTMQKVLQQLLRERIPVRDLATILETVADYIGATKDA
                     DVLAEYARMALRRQVTELAKDARGRISVFTIDPAVEQTLADSVQNTKQGLMLAIDPAL
                     AEQLLGRIGEQIDLQHAAGVNPACLCSPNIRLALRRLAEARFPGLMVLSYNEILPDVE
                     VISTGMVRLQDDH"
     gene            complement(49082..50152)
                     /gene="flhB"
                     /locus_tag="PKM94_00645"
     CDS             complement(49082..50152)
                     /gene="flhB"
                     /locus_tag="PKM94_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOD65174.1"
                     /translation="MADESFQERTEKATSRRREKAREEGQVAKSMELNSAAVICLGFV
                     TLYLMGPYIAGQALALMRATMANAPSLALADPTFTGVFRNHLLKFFIILGPVFAVLTV
                     IAVGVNVAQVGFRITPKAAAPQLDKLDPVKGFKRIFSVRSAVTLLRDLLKLTVVAFVA
                     YRVIAAEFPEFYRFADMTVAQIAAAMGKLALELALKVGAVILVIAVLDYIYQRYEFEK
                     SIRMSKQDLKDEFKDTEGSPQIKARVRQIQLQIARSRMMEAVPTADVVITNPTHYAVA
                     LKYAPEEHRAPHVVAKGQRLVAQKIKEIALAHNVPCVEDRPLAQALFKMCDVGQMIPA
                     ALFRAVAEVLAYVYRLKGQGVH"
     gene            complement(50158..50946)
                     /gene="fliR"
                     /locus_tag="PKM94_00650"
     CDS             complement(50158..50946)
                     /gene="fliR"
                     /locus_tag="PKM94_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOD65175.1"
                     /translation="MFEFVNFGANALQVFLLVIMRASGLFLMAPVFGDKSVPVLVRIG
                     LIILLAGVIASVIRQPQLPAFDSLWVLAGFAVKEVLVGLIIGLVFRLLFMGVETGGAL
                     VGYQMGFAMVELPDTDGSSQMPVIGKFWVLTATVIFFVIGGHHLVLRAFADSYEVIPP
                     GVAGTSGAVGELLIKYTAYVFVIAIKIAAPVMITLFLADVALGVIAKTMPTMNVFFVG
                     IPIKIAAGLAMLALSLPLFGYVLEKVTLHLDRELRVLYLAFGKA"
     gene            complement(51201..52232)
                     /locus_tag="PKM94_00655"
     CDS             complement(51201..52232)
                     /locus_tag="PKM94_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOD65176.1"
                     /translation="MQTLSLLGSMFGLALLSGLNLYAAALTVGLAVRYNIISLPLPLQ
                     GLEVLAHPAVLVIAGLLYAVEFIADKIPWVDSAWDAVHTFVRPLGAAALGLAAFQGMD
                     PVWETAAALVCGGLSLSSHSTKAATRLIVNQSPEPVSNSLVSLFEDVLVVVGSLLFLR
                     YPALGMAAVGLFLALMFWLGPKLLRLAKMEFAAVTGSLRGDEGAPVLSDTDLPDPFRR
                     ALNALPPTDPPRRRAWVYSGKGFSGGRFYAGLLVSGPARLAFITRRWFKVRTYEIALS
                     ATARIEFRKRPVLSVMHIESGRRVIKFYGTRDKAAALEALAEGLRAQLGRGEAAASSP
                     SSDPRTDLS"
     gene            52535..53950
                     /locus_tag="PKM94_00660"
     CDS             52535..53950
                     /locus_tag="PKM94_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD65177.1"
                     /translation="MSISHRALVAGGRKARPSRLRAVVCAALTLVLLAAPGRSAAGAP
                     GQLGTGRALIPITPENAPNVALLATLSEHTGGVWTAAFSPDGRQLASCGEDGRVIIRV
                     VDSLSHTRTLGDSEYWVVGLAFSPDGSQLAAGEADGFGGAYGPIRIWDAAADTLVRTL
                     SGHYGGVWSLDYQESTGMLASGSFDGTVRIWDPQTGAPVRILSGHTAAVLAVDFSPTQ
                     DLLASSGVDFTVRLWQPATGDTVRVLRGHTGNIGYVEFSPDGLALASSADDRTVRLWD
                     FSDGSLLWSRAAGQGWVNCVTFSGDGRLMATCGHDGSVVLRDASTGAELKRLAGHQGP
                     VLRGAFSPDGTLLATASWDLTVRLWGVETDLDGDGADNDSDNCPNVPNPGQEDADGDG
                     IGDACCCTVRGDCTGDAAVGVADLTYFIRYLFRGGPAAGCPSHADINADETVGLADLT
                     CLIGFLFREGPAPAACPSAWR"
     gene            complement(53996..54265)
                     /gene="fliQ"
                     /locus_tag="PKM94_00665"
     CDS             complement(53996..54265)
                     /gene="fliQ"
                     /locus_tag="PKM94_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOD65178.1"
                     /translation="MTQEMVVAIGREALSVTLLISGPMLLFGLIVGLVISIFQAVTQI
                     HEMTLTFVPKILAVALALLIFLPWMINIATDFTRHMFDLIPQLVG"
     gene            complement(54335..55087)
                     /gene="fliP"
                     /locus_tag="PKM94_00670"
     CDS             complement(54335..55087)
                     /gene="fliP"
                     /locus_tag="PKM94_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297884.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOD65179.1"
                     /translation="MKKLLVLGALVAAASLLAAADVSAQSLPKVSLQVGSADNPQELS
                     TTLQIVLLLTILALAPSILIMVTSFIRFVVVMSFLRSAMGINQMPPNQLLVGLSLILT
                     FFVMAPTVEKSYGEGIKPYLDKQISGKEAFDKATGPFRQFMLAQTREKDLALFVDMAK
                     LEPPETPEDIPLHVLVPGFVISELRTAFQIAFVLFIPFLVIDMVVASVLMSMGMMMLP
                     PVIVSLPFKILLFVLVDGWYLLVKSLVESFRM"
     gene            complement(55154..55714)
                     /gene="fliO"
                     /locus_tag="PKM94_00675"
     CDS             complement(55154..55714)
                     /gene="fliO"
                     /locus_tag="PKM94_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOD65180.1"
                     /translation="MNSKLTRRRSIGLALILGAAIAGLALINGGPASAERGVDLGRLQ
                     AAPNSETDAAPFAGSALPSLVRIASALAIVIACIYGGIYALRRLAGKRGGFGRSGRLE
                     IIETAAVAPRKTISLVRVADKAVLVGVTETAITLLTELSPEKTAEILAAPAAGGEAET
                     FPNVLKAASGKLRTLRLKRKDTVLTA"
     gene            complement(55704..55967)
                     /gene="fliN"
                     /locus_tag="PKM94_00680"
                     /pseudo
     CDS             complement(55704..55967)
                     /gene="fliN"
                     /locus_tag="PKM94_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(56252..57253)
                     /gene="fliM"
                     /locus_tag="PKM94_00685"
     CDS             complement(56252..57253)
                     /gene="fliM"
                     /locus_tag="PKM94_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOD65181.1"
                     /translation="MAKILTQDEIDALLTTVSSGESAPGAERGRDDRMRSVLAYDFKH
                     PNRVSKDQLRTLENMHDNFAGHYSSSLSTIMRTIVDVDLVSVDQITYTEFIMSLVTPS
                     CSCTFAAEPLDGLSLVDFNPTLTFSFIDRMFGGSGKILETERELTPIERSVMGRLTHR
                     LYRDLERSWENIVEIKVEQKSFETNPQFIQIVPPGETVVVVSFQVKIFQSTGLLTICY
                     PYVALEPIITKLSAQNWIDATKKRLQKSDRDVNLTNLNDVAVDLSAILLDTSIRMREF
                     LDLRIGDVVPSEKKINAPIEVFVNRRRKYTARPGLAGKKRAFQVIDVCERSSEEKKQ"
     gene            complement(57266..57871)
                     /locus_tag="PKM94_00690"
     CDS             complement(57266..57871)
                     /locus_tag="PKM94_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOD65182.1"
                     /translation="MPNESDKNEKTAVTPAAAAPPPDQPAPAPGGKKKLLLFGGIGLA
                     AVVVGIVLALGVVKPMLAPSPETPAGAAESAAPAPHAETAPAETRKESGGGHGGGADA
                     ARIFTIDDLVINPAGTGGSRFLSVSIGFEVKTEAAMIAFRAREPAIRDALITILSSKT
                     VAQLTDTRQKEITRYQIKKRVGELMKTDELAGVYYTDFVLQ"
     gene            complement(57855..58091)
                     /locus_tag="PKM94_00695"
     CDS             complement(57855..58091)
                     /locus_tag="PKM94_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65183.1"
                     /translation="MIRVTRHNEVPFEYTAELIRFLESLPGPVTFVNVDGEIMVRLSD
                     EPGRGAPDQRDRRPGAPGAGRRARAIGERHAERK"
     gene            complement(58099..60039)
                     /locus_tag="PKM94_00700"
     CDS             complement(58099..60039)
                     /locus_tag="PKM94_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOD65184.1"
                     /translation="MMASLYAGVSGLKNHQVKMNVIGNNIANINTVGFKTSRVNFQEA
                     LVQTFKGAGRPSAITGGTNPVQLGLGMEVASIDNLFQQGGLETTGQILDLAIQGSGFF
                     ILGDANGNRFYTRAGAFGLDSQATLVDPSNGLYVLGRMADNTGQIPSLATTGPIRLPF
                     GQQDPANATEEIWLSANLDASASDANAALVESGDSNVLLVSGTALDGVGGTHRVSITG
                     DQAIADTVTSATAGMTGAQTLGALGVTDFSDFSLTIDGARTETISGLSATTTVEELIA
                     SLNQIAGVSASLDGSGAITIMRTRAGDHATYNIVSSPAGAGDVLNTIFGAGLGATVAT
                     SGGADTTLVATDTFTPLQGSGTAGGPFVTQLGLVVDERTGLVTGLSGLGDGGVEITAG
                     QAGIGATNGNDLVIDTESTTHTASITVFDSQGGRHTLSIEFFRSALTNRWEWSVATLG
                     TETVLTGGSGYVQFNPDGSLNSFAYNGGNEAITINPNNGAENMEVVVNPGTAFSFDGL
                     TGFASGTHTASIVRQDGYGVGILEKIAIDKAGNISGIFNNGVTRLLAQVMLSDFFNQS
                     GLRKAGKSMYQESANSGSAVEGVAGQTISGQIFSGALESSAVDIAQEFTGMITAQRGF
                     QANARVITTSDNMLDELVNIKR"
     gene            complement(60140..60538)
                     /locus_tag="PKM94_00705"
     CDS             complement(60140..60538)
                     /locus_tag="PKM94_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOD65185.1"
                     /translation="MKITPQPIAVGQRPVNLIEIANRTAPAGPAQAGEGSFREMFSRE
                     LAADRAVHFSKHASQRLFSRGIALSDATLAKIADAIDRAAAKGSRETLVLTDEAALVV
                     AVPNRTVITAFDRDNLREGVVTSIDSAVIL"
     gene            complement(60572..61276)
                     /locus_tag="PKM94_00710"
     CDS             complement(60572..61276)
                     /locus_tag="PKM94_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOD65186.1"
                     /translation="MSEITNTTVGGLMSAAANSGQTGSLQQLGKDDFLQLLVTKLQNQ
                     DPLNPMEDEDFIAQLAQFSSLEQMTNIAEGIAESNKWDYLQMQSINNVMAAGLIGKEV
                     EASYDTIYFDGSKEPSLTITTDRFVASVEIKITNADGEVVATLTDENLASGKHTFTWD
                     GTDAQGNRVPEGSYAVAVRAVNGAGAEYTPKLGMRGVVEAVTYRDGAAYFRVDGMEIP
                     FGDVAAIAEEGQLGGE"
     gene            complement(61286..62962)
                     /locus_tag="PKM94_00715"
     CDS             complement(61286..62962)
                     /locus_tag="PKM94_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOD65187.1"
                     /translation="MPTLSPDIMNLLLGVAPLPTVEPSGATAGKEAGAPPELLFGALM
                     QTALGPSRVTSLLPLAKTASMPSGTQAASVPGQTARPADDTLAAACRPLVPATRDGER
                     ALLDDLLVSPGWDFEALETTSGDIYDKARSVLQGRAAIQAAAVSDEYSPAATNPPGSI
                     GTGTFVPTPAALQLIQDLTPQPLDTLPAGAYIITDARAAEGRLMLDLSPKDPGHPAIR
                     ISLPLPQPSEIPAPHVSVPASAARVPLESGASPTALESWFEKVSLKELVVTPAAGHGS
                     AQAGGAAASSEAPCKFEILAENAGRDVVIVTTPAGSDIRFRLLQNRAARPASPGTPGA
                     ALAGAKTTPRPAAAAPDTADGHFTDSRLFPLTREIRFLTLAGGRGKAPDASGEAAATL
                     AGSAKGAGADDLRTAAAGERVQTPPARFTLPADVKTALRPGGDSVELRIDPEHLGPAR
                     LHLTVRDEHLSARVIVETARAKHTIENSLDRLVETLARADIKVDHIEVAIGQQGARGQ
                     WFAQHAPHWPRSGASGGRFDRDDETAPAPPAVPATGSSGYLGAGGVDVLA"
     gene            complement(62965..63705)
                     /locus_tag="PKM94_00720"
     CDS             complement(62965..63705)
                     /locus_tag="PKM94_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65188.1"
                     /translation="MTDQNPSNNPPAPAATPAPTPNPPPAKKGLLTYLLYGGLGLAVI
                     AAASVGTLLLLGGKSTAPVAPGDTDSTLAGNRAAQAAQLTDDQILDSLLAGEDDSDML
                     ALIQENLNVLDYDPAESAAAGDTAPAIEGAPGTADHLAEEQKRLAQWESQLKTRENDL
                     VRRENEVSRQMLVLEQAESARTAGLAKLYDGMDSHAVAKLMTNLDDETVVSILPRMKA
                     KNASEVLQLLPPERAARLSKQMITIAEK"
     gene            64163..64492
                     /locus_tag="PKM94_00725"
     CDS             64163..64492
                     /locus_tag="PKM94_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOD65189.1"
                     /translation="MKIYIGNMSYSTTEDQLRQAFEAFGEVSEVNLVMDRDSGRPKGF
                     AFVEMPNQDEGKAAIAAMNGKEFDGRTLNVDEARPRREGAGGGGGGRGGRGGFGGGGG
                     GNRRGGW"
     gene            complement(64591..66384)
                     /locus_tag="PKM94_00730"
     CDS             complement(64591..66384)
                     /locus_tag="PKM94_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65190.1"
                     /translation="MRDKIRWLWRYWRHYPKVLVILLVLTPLQTALMVALPRLIGLTI
                     DYLNTGAAPRTGAAGWLSELGRAIGLNTTAAYAVTFILFGLIAYALYAYFQSWRAWMN
                     TRLEWHFRQEAFDGITAKGPDFFNHYRSGDLLTRMTDDVQDKLSWFACSGIFRLYEAT
                     LAVVFIVASMLTISPGLTAWTALPLPFIVVLFFRSSSLLDRRYDALQKRISDFNDVME
                     ACFSGIRVVKAYVREQAQKVKFTGAAEERRKAEIDAVRIAAVMDSLYHYLWQFAVVIV
                     LLAGGYRVLYADLSIGSIATFIYYATWLVFPMFDIGQFLVKSRQSAVSIDRLVELEKV
                     PPMVADIGRLSPNGEGQGRLTFENVSFAFEGMERKILEDVSFEIAPGQTAAIVGKVGS
                     GKSWLVNLIPRLVDPTAGTVRLDGLDLREYRLEELRRAIGFVPQEPVLFSDTIRNNII
                     FGREGISETLLEWAIDVARLRGEIAGFPQGLETPIGTRGVAISGGQKQRLALARALVG
                     KPRILVLDDCTSALDSRTEAELWERLHEVMPEMTAVLITHRPDTLERADRIFVLDSGR
                     VVEQGAHSALIDAEGDYARIYRRLQLAEAVN"
     gene            complement(66374..68197)
                     /locus_tag="PKM94_00735"
     CDS             complement(66374..68197)
                     /locus_tag="PKM94_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65191.1"
                     /translation="MTAELYEHEDIGKDEKDVSVRRAFRGLLPFLAAHKKGLALCLLL
                     LVAVTAMTLAWPWLIQQAIDKILRGQLDRPAAERDFSPLVKVGLTVVLLQIASLALMY
                     IQRVKLETIGQNVMLDLKRKLFDHILSLDVSYFDKNPVGRVMARVESDTESLRMLFTN
                     TVVLIVGDVVLIVGILGVMFYHHPRLALVLSLSLPALALMIYVFHRLTSHRFLEVRKR
                     AAEVTAALAEFLHGMSIVQIFHRGAYARARVSHASERKFREDSYVNIGVCIFFNLLGY
                     IEFVKVGLVLLLGSMWGVSSGTMVMFILLIWREFEPIARTAEQLGVFQKGIAGARRIF
                     ALLSIRPKVLDPARPVDWPGLHRTIRFENVWFSYGDDDNWVLRDVSFDIPAGKRVALA
                     GVTGGGKSTVINLLLRLYDPQRGRITVDGVDIRDLRQADLRRRFALVLQDIILFPGDV
                     AGNIGLENGAVSEAQIVEASRTVEADAFIGRLPAGYKTEVSEKGSNFSRGERQLLSFA
                     RALAVDPEVLILDEATSSVDPETERTIQASLRRLTAGRTSLIIAHRLSTILDADQILV
                     IRRGEIIERGTHTELILANGYYSKLFHLQFKHRNGVMTHAG"
     gene            complement(68527..70248)
                     /locus_tag="PKM94_00740"
     CDS             complement(68527..70248)
                     /locus_tag="PKM94_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65192.1"
                     /translation="MSIPRSTAVAAAAVVTLAAALMAGAPQVISFQGRLTDDLGIPVA
                     DGPADVRIIIWNDPTAALPENEVWNSGVVTVTTVGGLFSLNLGVSPMPALTPDLFADT
                     NLWVGLSIEGQAEGQPRIRLGSAAFAFRAVRADTAAYALASPGGTSDHGSLTGLLDDD
                     HPQYVRVDGSRALIGDLYMSGRLIRNLAPGQLASDAIRYDQAMKLGDPAGGDLAGTYP
                     DPQLAANSVGLAELAAGSVNSEKVLDNTLSALDLANGSVQTAEIADGTIVDADISPTA
                     AIAVDKVAGTAASLSGAQTVTGAKTFVGGVKIGSAGAPSASAALEVSKEFLSPGLVYG
                     LSLGAANNGAGSVHSLYAAASGPVAHSGYRTGVYALAGAADATAGYSRGLAAFGLGGE
                     TAYGVYAYASAGATNWAGYFDGDVAVTGTLSKAAGSFRIDHPLDPENRYLQHSFVESP
                     DMKNIYDGVVTTDAAGYAVVTLPDYFEALNRDFRYQLTVIGDFAQAIIAEKVAGGRFV
                     IRTDKPNIEVSWQVTGIRHDPYAETHRIAVELDKPARERGRYQNPEAYGLGSDRSINR
                     AERTVQE"
     gene            complement(70408..70884)
                     /locus_tag="PKM94_00745"
     CDS             complement(70408..70884)
                     /locus_tag="PKM94_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65193.1"
                     /translation="MSVLVRCLAGLGAVVLAAAAALIGCAPPPPVADQPTVPRGFYDE
                     VWVEPRIVHADSVFTLIRADRKDSVFVRTNEVDPNAWAPALEFKVAPDTCFVSVTLHF
                     LESDRPALPLLARYLPKGYYKLTVNNPLRFGETVYRGSCLLGATVCGRRQTVPIVR"
     gene            complement(70881..71507)
                     /locus_tag="PKM94_00750"
     CDS             complement(70881..71507)
                     /locus_tag="PKM94_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65194.1"
                     /translation="MTRAIPAGCLAALLLGAAASAQTIEGLQVPPADSAEAERLRIWA
                     AAEGTSRCRMIVEVVDSANRVVRRLVDFLAAPGYYNFYWDKRDDSGLRVPEGKYRGRV
                     DYCGALSWENLTARYSLWELQSDLTPFDPDNPFRIGVVLTADGAPVAAEILNRRDVHF
                     DVMLPDTVLDIGTHWIPYRPPERMPRGNYTIRIKVGDYEYRREVTYLP"
     gene            71644..72225
                     /locus_tag="PKM94_00755"
     CDS             71644..72225
                     /locus_tag="PKM94_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65195.1"
                     /translation="MMKFWESMATGRRRGKVLLALIVLTLAVYGHMIFVSIPAVSARA
                     GGMQILDLMPVGYSPAYAAQLFERLGEAGRRAYLTEQIPVDLLYPFLFGVTFAWLLLY
                     LMWRVSPRRTWALGAAAMPLLAGLFDYAENAAIIAMLASYPDFSPGLVRWSSGFTIAK
                     SCLTTLSVVLLLGVLAAAAVRRFSRSRHPAEAA"
     gene            72222..72626
                     /locus_tag="PKM94_00760"
     CDS             72222..72626
                     /locus_tag="PKM94_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HOD65196.1"
                     /translation="MNRITAVEFRARLEALCTQRNNPGLPRRRRDQLILLGSIVRELV
                     PGRTYSEPDINAVIAAWLADVGRSIDIDFVSLRRQLVDEGFLARDPAGNAYRLEPQRT
                     ADLFEPETAAIDPAQAVREARERREKRRQAHR"
     gene            72663..73484
                     /locus_tag="PKM94_00765"
     CDS             72663..73484
                     /locus_tag="PKM94_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65197.1"
                     /translation="MTGRHSDLSAAHIAAFRRDIRAFYRRHGRHLPFRETNDPYAITV
                     AEIMLQQTTVERVTPYYEAWLRRFPDWQTVAAAPIRAVLAAWSGLGYNRRALYLQKLA
                     QTVVGEYGGELPSDPAVLRRLPGIGPYTANAVAAFAFGRRVAAVDTNVRKVLLHRFGL
                     AADARPAEIQRIAELVLPRTGIRAWHAALMDYARLGLPPRESRRLRVRRQPRFEGSIR
                     QIRGEIIRRLTRRSRVSLGTLARDLGRSMAEVRAAAEGLARDGLAVATGRTVRLP"
     gene            73610..76660
                     /locus_tag="PKM94_00770"
     CDS             73610..76660
                     /locus_tag="PKM94_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD65198.1"
                     /translation="MYLAKPSAPVAPFRVLQALLVCLVLIATIPRPAAAATINVPADS
                     ATIQAAILGTLPGDTVLIAPGTYHENISFIGRSIVVRSSGGREATIIYGDSVNAAVRF
                     ETGEDTSAHLEGVTVRRGGAAGVLCTGASPTIHDCTIDSSTIGIDLAASQAIIGQCDI
                     LYNSSHGIRLTGSLATVTMCTLSDNGGSGIYGENAAVFTGNTLRRNGSYPLVLGAASI
                     GRLAAGNQFMVNAIQAFGVLGGTVGDSAVWKPLSGISTFVILGGGDLSVAGPLAPTLV
                     LDPGVRLEFAVATGTLSVGLGNPGTLIADAPSTNPISFVPHASIAQWRGIRFESNARD
                     EECLLRNCLVRRSQGIVCNFSSPVFDSCRIDSCAGDGLSLIGSQSVIGNCVIADNSGV
                     GIRLSSSQVPVTGCEISGNGSYGVYGTMLTAFSGNVVSGNAAAPLAVGPTSAGRVDAT
                     NSLAGNGDPTIYVLPGTVSASGTWRAHPGIERYVVASTAAVNVQGAATPVLTLAAGVR
                     LEFGHPSYGLIVGAAAPGGLIAEGTEELPVLLVPAGPAASWGGLAFGASAVDSLCRLG
                     HTVIRRAQTVACTDASPVLDSCRIDSCLGDGLALVNSQAQLTGCTIADNGGDGITISG
                     LPPGISGCAITGNAENGVVGGPLEVFAGNTIGFNGGHALWLEPNSVGRIAADNLFSSP
                     DQTIAVQGGIVGESAVWRAFPNVRRYLAGTSGIVYVQGAGTPVLTLSPGLRLEFPSAS
                     FGLRVGYTAAGGLKADALGGEPIVLTRDTNTASWAGLRFSDQTIDSASYLKNCIYRSA
                     RSLYVADASPMITDCTIDSCTESGLTLTNSVSRVLRSRITDNAVHGVSVSGGTPYLAS
                     SLISGNAQCGVYCANDAKPVIGSVAGAGCNLSRNGTFALYNDGAQTIYARENAWGDDD
                     SAGVAALIWDQVDDPSKGWVQFMPFVLGYVCGDANGDGVGNPGVADVTYLVAYLFRGG
                     PPPPEIDAADVNNDGAVRISDLAYLIAFLFRSGPAPYCWEYGPS"
     gene            76805..78841
                     /locus_tag="PKM94_00775"
     CDS             76805..78841
                     /locus_tag="PKM94_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOD65199.1"
                     /translation="MRRTGLFLLFAILTPMWIAASGERKPTATGTITGTVTDKETGRP
                     VTSASVLVVGTAFGAQTDLDGRYRIDNVAPGSHLLRFSHVEYRTVELAGVEVAEGSTA
                     VRSVALEKNGAVKSDVRILGTKDKLGVPESSSQAPVTKEEIPMYRLPAEDQALRQPPD
                     AAAAGHGVEIFMRGGRAGEAEYITRENSLPPAHGGSAIVNGQPYDAMFFRDYGVNPFV
                     DTDDDHLSTFAVDVDDASYIMTRSYLQDGHLPPAEAVRTEEFINHFTYDYDPPRRETF
                     RVYADGAPSLFGEKTRLLRIGIKGREIDPEERKPATLVFVIDVSGSMARENRLGLVKQ
                     SLRYLVDQLLPDDRVGIVVYGSSGRVLLEPTGAGHRTRILRVIDGLEPGGSTNAEEGL
                     RLGYRMADRNFVRGQINRIILCSDGVANVGKTGPEEILRDIRRYAERGITLTAVGFGM
                     GNYNDVLMEQLGNKGDGFYAYVDDAAEARRVFIDNLTGNLQVIARDVKIQVEFDPRAV
                     RSYRLLGYENRDVADRDFRNDAVDGGEVGAGHQVTALYEIKLWSDGNRRDKHAIIDRR
                     EDDDGARPGDLGIVRIRYKDPDRDRVAEITGRITDEVFDRAFAEADPDFRLAAAAAEF
                     AEILRHSYWARDSRLSDVIRVAHRVRSETGSEEAAELADLIARAMHYEDELAER"
     gene            79032..79940
                     /locus_tag="PKM94_00780"
     CDS             79032..79940
                     /locus_tag="PKM94_00780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOD65200.1"
                     /translation="MAERFAARYRGAVDDYPAFLATLTRPPRNTFRVNTLKADVGATV
                     ARLRAIGLTITPLPWFEAAFAADPHINLAATLERFLGTIYIQEAASMLPPLVAAGELR
                     GARTVLDACAAPGSKTTLAAAIMANRNVIVANDRSYGRIRALQFNVHKFGAINTLITN
                     YELQLFPKASFDTVLLDAPCSSSGTVRKSPKVLETWTSAVSAGYAERQKDLILRAFDL
                     LHPGGTLVYSTCSLAPEENEGVVDFLLRSRPAETVPAALPGFRFAPGLAEWEGRRYLA
                     PVARCGRVWPHANDTDGFFVAKVVRC"
     gene            79934..80398
                     /locus_tag="PKM94_00785"
     CDS             79934..80398
                     /locus_tag="PKM94_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65201.1"
                     /translation="MLTPCPDEIRRQLLACLAERFGFDTALLEPYELYLSESARQKIF
                     LGPKTDFPLARTDTGGVLIARAGATIKPATSFFQLFGRHVTRNIIHLERGQVAPFCSG
                     SDLELAENQIGGAERGFVMVVWEEHCLGCGLLKGRTLENQIPKANRLTLEQW"
     gene            complement(80561..81949)
                     /locus_tag="PKM94_00790"
     CDS             complement(80561..81949)
                     /locus_tag="PKM94_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOD65202.1"
                     /translation="MAPLDLAARVPRDLSLPLPLDPAVIEVFLVVLFLLHIVFVNLMV
                     GGSLLTVVCEIVGRSHPRYDALARRIGATITVSKSLAVVLGVGPLLCINLLYTLYFYS
                     ANALTGYAWISLVPLVITAFLLSYLHKYTWERWTGPAKNHHIVIGAAGAFFFLMIPLI
                     FLANINLMLFPDQWSAVRGFFSSLQYGNVFPRYFHFLTASLAVTGLFLAGWFGRRRFP
                     VEQLLPAFTRAELRGLFYRLAFWATAAQLVFGPLLLLTLPLRGISWTLLAVVGVAAVF
                     VVLILALLWRETRAGNPAIGRRYGAVIALFTVLALFMATGRHLYRETALSRHRLAVAD
                     RTATFAAVELAARMRLAAGLGVGETVGGAPTGPSVFSNCAACHALDKVLAAPSIKEIY
                     SLYANNPAGIVQWAKNPGKKRPQFQPMPSMAHLGDEKLQLVADYMLSLGGGDGDANAA
                     DQPAGSPEQSRP"
     gene            complement(81965..83374)
                     /locus_tag="PKM94_00795"
     CDS             complement(81965..83374)
                     /locus_tag="PKM94_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOD65203.1"
                     /translation="MDFPLFYLDFIGNRLLMAAVAITHVMINHPMAVGAWPLVALLEW
                     WGRRRGDLDLDALAYKITFVLFIITTTFGALTGVGIWLTAALIAPFGIGSLIRVFFWA
                     WFIEWLVFISEVGLVIVYFLMWQRWREGRWKALHNAVGAVLAVGSWLTMAIIVAILGF
                     MMGTGSWAADRSFFSAVFNPLYLPQLVFRTGLAMVTAGFTVWFLTFFFTRTRPELRRR
                     VVRIVSGWVLAWTPVVLIGAVWYWRRVPAPMAANLDVAFLTQRLTQWYDTFLYVVAAV
                     LALTVLIALAGLARPALIPRAVLVIPFLLGLYTLGHFERVREFIRKPHVVADYMYSNG
                     VTMSELPIFQRDGILTYSAYSTVRQVTSANKVDAGRDVYMLACSRCHTVSGVNSVLTR
                     FRNLYGDTPWDPAVLSSFIAVMHNTRTFMPPFPGAPAEADALAAYLRDLQERPRGLPG
                     AQAVGYSPRAAAAAAGPGQ"
     gene            83576..84772
                     /locus_tag="PKM94_00800"
     CDS             83576..84772
                     /locus_tag="PKM94_00800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOD65204.1"
                     /translation="MTYPLLTLKAGRENAPANRHPWIFSGALAEGKPEVPHGALVRVA
                     EASGQVIATGTYSAKGMIAVRVLAWEEAPITREWIRERVREAVERRRLVGIGADETVT
                     GYRAVFGEADGLPGLVVDRYGEVAVVQFATAGAEGLREPILQALADVLTPAAIYERSD
                     LPARTEEGLAPASGLLSGVLPERVEFAEHGLRFTADVTGGQKTGFFLDQRDVRRQARA
                     LAAGRRVADLFSYRGAAGVAALKGGAEHVHCVDSSAAALAAVGPEAEANGLAAARITA
                     EEADVFQWLGQRSEPEFDMVLLDPPALIKSRRHAEEGRKGYHFLNRAALRILRDQGLL
                     VTSSCSFFFTEDDFATTLRRAAAQAGVQLHVLATVRQAADHPVALHFPESFYLKSFVC
                     QVRRRV"
     gene            84822..84895
                     /locus_tag="PKM94_00805"
     tRNA            84822..84895
                     /locus_tag="PKM94_00805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84856..84858,aa:Arg,seq:tct)
     gene            84992..>85224
                     /locus_tag="PKM94_00810"
     CDS             84992..>85224
                     /locus_tag="PKM94_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOD65205.1"
                     /translation="MEDHLKRLYLLVDELKVESHWKTYVDAFVFGCKIRNLAPRTLNV
                     YAERLGYLVRYLEEKGIDIEDITRQNIQDYLLSL"
BASE COUNT        13878 a        27480 c        29620 g        14246 t
ORIGIN      
        1 cggccagcgc cgtcaccgag accagggcgg aggcggagtg ggtgcgggag tcctgcgcca
       61 agggccccgc gctctccctc cgcgtgtcgt agtagttgta ggtggccgag aggtaggcaa
      121 gctcgggcca ggagcggccg gccccgagcg tgccggacca ggtttcggtg gtcgaggaaa
      181 tcccgccggg gagcgtgctc cggcgggcaa ggttgttgta gttggcgcgc acgttcagcg
      241 gcccccgcgc gtagccgctc tcgaccaccc agttgcggct gagcccgccg aactgcggga
      301 cctcctcccg atcgtagttc ttcacgcgcg agtagaccac cgcgagagtc ggcaggttct
      361 cgtacgcgac cgcggcggtc gcgcgccagt cgcggtagta gacgtcgaac gcgtccccgg
      421 tcgcgatcga ctgcgaccgg cgcgtgtacg gcgaatagcg ggccgtcagc gtgtagccgt
      481 aactctttag atcgaggtaa tagatcggct gatactcggc gtgttcgaga aaggggtatt
      541 cgcggcgctg gaggttggct gtgagccgga ggaggttctt cgtaaacagc tcgtcctcgt
      601 aggacaggag gagcgattcg cgcgtgacgt tgtcggactg cccgaccacg gcggtgtcgg
      661 cgacgagttc gccgccgaga gaatccgccc gaacatcgag cgcttcctgc ctgaggtgtt
      721 cccaggagag gctggcgttg ccgaacaggc gctgtgcgcg caccgcggca gcgtcccatc
      781 ccggcggcag cagaatggcg gccgacagca gcagcgacga gcgcagaaga cggcgacgga
      841 ggaggggcat cagaagtccc ccggcgccgg cccacccgga gcgctgccgg gggcggggtt
      901 gtgcacgttg tgggaagtgg ggctcgagta gtcgcggccg tggcaatcgt aacagtgcgt
      961 gacatcgccg gccgcgtggg ccgggacgtt cgacggcagc ggactgagga tgagcccctc
     1021 gaccgaggcc gagccgcccg ggctgtccga ggccgggtga caaccgagac agaagcggcg
     1081 gacctgggcg ttgtcggttt tgatgtcggt cagcccgtac cagccgggac gctggtcgga
     1141 gagcaggtag tggttgccgc cgagaccgcc gtagccctcg gaaccgtgcg ggttgtggca
     1201 atcgtagcag gggaggcgcg cgccggaggg cacgtagccg ccgccggatc tcacgccgtg
     1261 accgctcatg cggccggggt tgatcgagcg gtcatagtag tcggcgatcg tgcgcgcggt
     1321 atcgtccatc gccaccgggc cgtcgagact gtgacagttc aggcacaggc tgtagtcggc
     1381 cggcagcagg ccgacgtcgc tcccgacgat cccgttgtaa gtcgtgtcga gcatgtcgtg
     1441 aaggttgccc gacccgtgca cggcgtggca gttggcgcag gaaccgtcgc cgtagaagtc
     1501 cttgatcggc atatcgggat cggcgaagtg gggcgagtac cgggggtctt cccagatgat
     1561 gcgtccgggc cagcgcacgc ggtgctcgac gccgggcacg cgcccgccgc cgctgccgaa
     1621 ttcgaaatag ccgggatcat cggagccgag cggcaggcgg tcggactcct gggccggata
     1681 gcccgaggtg gcgccggtgg gacggtcggc atggcaaccg ccggcggagg cggcgaaaca
     1741 gaggcggttg gcgttcggct cgaagaggcc gtagtcgttg ccggcggccg gatcgtggct
     1801 ggtgtggcac tgggcgcaac tgccgcgcgg gagctcggtc aggcgccaga cacctgtagc
     1861 ctccgagccg tggggcgaag cgcggtagtg gccgttgtcg gcccggagcg cggcggccgc
     1921 cagcagcgaa ccggcagcgg ccgcgacaag ggcggcgcgc aggcgagggg tcgggcgaaa
     1981 tgctctcgtc atacttgctc cgcaacctct cactgattgt ggcattgctg gcacccggcg
     2041 agatggccct cgccgccttc ccggtagggc caccgcagcc cgtactcgaa cggcccgccg
     2101 tgcggacggt ggcaactgat gcacacgact tcattggagg cggccgcttc ccgggcgctc
     2161 acgtagtcgt cggccgcggg cgcttcgaaa gggacgcgcg ggatgcccgc cgaggcgcct
     2221 ccggccggcg ggaagccgtc gccgataccg gccgtccagt gcgccgggtc ggtgtggcgg
     2281 tcggtgaaat acgcgtcgag cgcgatgtcg ctcgggtggc cggtgaaatg agcgggcgga
     2341 acgccggcgt tgtccgcccg cgcctgatcg tggcaggcga gacaccacgc cgaggagttc
     2401 tgccggtagg cgatgttgtc gcgctcgtag gcggcaacgg tcgccgccgc gctcggcgga
     2461 tcccccggct gaaaccgcga aaagacatcg ccgccgtcga ccagcacgat cgaatccccc
     2521 cggtcggcgg ggtcgaagcg caggttgcgg tagttcgagt tgccgtggac cgcgtggcag
     2581 tgagcgcagg tgaggtcgac cagttcggcc gaggtttgga gcggggcgag cgtgggaatc
     2641 ccgagattgt ggccgccgcg gttgtcggcg cccaaaagac cgaaccagcc gcccgcgctc
     2701 tccccggagg gcgtgccctg gtacatctgg acggggctct ggacatcggg ggcggtcgga
     2761 ttgctcccgt cgtggcacga cagacacagc tcgttgatcg agcttttcag caggaggacc
     2821 gagtacggtc cgccggcgac cggttccccc tgttcagagg agtgctgcag gtggcagtcc
     2881 tggcacatca ggctctcccc cgtgtgccac tggcccgcgg ccgcggccag gggaagaagg
     2941 acggcgatgg cgcagacagt aactcgcatg gccgttctca ctgcacgcgg tagaagaggg
     3001 cgcgcccgaa ctcgtcgagg acgagcacgc ggttatcgat ggtggtggtc atcgcggccg
     3061 gccgaaagag cggctgggga aagggcacgg cggcgaccgc gccgccggcg ggatcgaaga
     3121 tgcggaaggc ggccgcggtg cggtcggcca cccagatcag gctgcggctg tcggccgtgc
     3181 cgccggcggc ctcccagccg cgctcctcgc cggggctgct ccagccgaaa tacaggagga
     3241 atttcccctc ggcggagaaa agctgggccg ggtagttctt ctcgtcggtc accacgaacc
     3301 ggccgtcggc cagcggcacg agcgcgctcg ggaccaggac tttccccttg cccgaaccgt
     3361 ggggaaccgc gatcccctga tagccccagc tctcgtcgaa gcgcagaacc tcccccgtct
     3421 tccggtcgag cgccagccag ccgcgctcgg gggtgcggag gagctggtcc agccggcggt
     3481 gcggttcgag aaccggcgcg agatcggcta ttgagtcgag ttcggccggg ttgtcgcgga
     3541 tcagccgcag gatgcgcaga tcagctgtgc gggtgaagac gagttcattt tcgccggtga
     3601 aagccaccgc gagcgggcgg cccaggtcga cggcgccgcc ggccgaccag agcgggcggc
     3661 cgaagtagtc gatgacggcg atccggttgg cctggggatc ggccaccgcg atctgctcgc
     3721 cgctcggcga cacggcgagc gagctggggc gttcgaggcg aaggccgtcg gccgcaatga
     3781 caatctcgcc gtcgagcgtc tgggcggaga cggccgcggc gaacaggaac acggcgctaa
     3841 gggcggccgc accaatcgcc ggccctcgcc gacaccgggc gcgcggcgat gaactgccgg
     3901 cgagcccctg tgcttccgtg tcgattcgtt tccgctccat ggtcgtcacc ccggccgccc
     3961 tcaatgcgag aggctgtggc aggtgataca gagtttccgg tcgcgatcgc cgaggaggat
     4021 cttgtcgtac tccgaggagt gcggcgaatg gcagccgaca caattgagat cctgcttggt
     4081 ccgcgggtcg atgacgccgg cgcccatcgg gtggtagtgc gtctctttcg cgtcggcgtg
     4141 gcaggtcatg cacagctcca tgatgtcggg ggtcgagagg aggcgcttgt agtcgccggc
     4201 gtggacctca tggcaggtgc ggcagtcttc gaaagccgcc tggtgtttga acttcccgct
     4261 gaactccggc gtgttgtggc aggtcaggca gacggccacg ccctgctttt tctgcagcgc
     4321 gccggtgtag ccgacgtgtg gaccgtggca gccggtgcag ccgttgtcgt cgccgaccgg
     4381 cgcgtgcacg accggccggt cgagcgtcct ggcaaaatcc tcgtggcagg tcaggcacag
     4441 cgtcttgacc ggcgcgataa gccgggcggg ctgtttgccg acgtggcagg aaccgcactg
     4501 gccggccgtg aagggggcgt gcgctctggg catgaggaga cccccgccgc ctttcggggc
     4561 ggcgtgcgcc tcgtggcaat tctgacagtc ggccgccgcg accgcaaaac ccgaatgcgc
     4621 ggccgtcatc tcccccgccg aggcgtcgtg acacgaaccg cagaggttct ctttcgaaac
     4681 ggtcagcagc gacgcggtcg cgccggcatg gggctggtgg caggcggtgc attcgccgct
     4741 caccaccggg gcgtgcggct ggccgttctt gatctgctgc tcgaggtcgg cgtggcaggc
     4801 gagacagaac gtctccgccg gcttgttctt gagcgccgtg aaggccgagg cgtgcgggcg
     4861 gtggcaatcg aggcactggc cgtcggcaaa cggccggtgc tgcaccgcga gatccatctg
     4921 cgtgcggacg tcggcgtggc agccggtgca gagcgcgccg ccctcggctt tcagcatctt
     4981 gtcgtagtcg gcggcgtgga cgccgtggca ggcgccgcac tcgcccgcgg cgaagggggg
     5041 gtgctgatgg gccaggccgg cggattcgcc gaccttctcg tggcaggccg agcagaccga
     5101 ggccatgtcg ccggacagcg ccgcttcccc gttcggcccc ttcgcgtggc acagatcgca
     5161 gctcttctcc gcaaacgggg gatggagccg cgcgcggacg agtttgggct gttcggagat
     5221 gtgggggttg tggcagtcga ggcaattggc ggagtcgagc gcgaaaccgt cgtgagccgc
     5281 ctgcaggccg gcatcgaccg catggcacgc ggcgcacagc tcaggggcgg gggcggtgac
     5341 gaatcccgga ttggcggaac cgtgcggcgc atggcaggcg tcgcagttcc cggcggccac
     5401 cggagcgtgc acgccgggcc ggcggaattt ctccgcgagg tcgtcatggc acgtgtaaca
     5461 caggtcggag ctgaggccgg tcaggcgcgg cagctgcgag acgacgcggc cgtcgaccac
     5521 ggcccgctgc atgcgcccgg tcacgaggtt ctcctgcatg gcgccgtggg ggttgtggca
     5581 gccgctgcag tcgcccgcgc gagccggcgc gtggacatac gcggcggtta ctttcagatc
     5641 atcgtagtcg tggcaggtcg cgcacacgtc cttctcgtgc gccgccagca gatggggagc
     5701 attggcgccg tagtgcggga catggcagct ccggcagtcg tcggcggccg ggggatgcgg
     5761 cacggtcatg cccagccggc cctgcacgtc ggcgtggcag cccacgcagg cgtccgcccc
     5821 ctcgccccgc accaggtgcg gcgccgtcga gtagtgcgga ctgtggcagg cggcgcagtc
     5881 ggaggtgagg tagggctggt ggccggagag cgcgccgagg gcggcgtcgt cgacggcgtg
     5941 gcaatcggcg cacagctcgg agggatcttt ccgcagaatc gcggcggtct tgccctcgtg
     6001 ggaggcgtgg cactgccggc agtcgtccgc ggcggcgtgg acggtcgcgg ccgaatcgcg
     6061 ggcggcttcg aaatcgctgt gacaggacag gcagagatcg gcctcggagc gcgccaggag
     6121 cgcggcgttg gccgagccgt ggacctcgtg gcaggagctg cactggccga ggaccgccgg
     6181 caggtgcggc gtgagcccct tgcccgtgag cacctcgctg tggcattggc cgcacagttc
     6241 ctcctggggc gccgtgagaa ggctcccgaa acgggaggaa tgcgggcggt ggcagtcggt
     6301 gcagttgtcg ccggtcatgg cggcgtgcgg gtgggcgagg ttcgctttct cggccacgtc
     6361 cgagtggcag gtgacgcatc cgtcgctggg cgacacgccc tccgcgaagg ccacccggcc
     6421 ccgatcgtcg gggaggctgt ggcaggtctc gcagtcgccc gaggcgaacg gctcgtgggt
     6481 ccggtcggag agaagtccct tgcgcccggc ggcgtggccc gagtggcatt cggtgcaggc
     6541 gaggccggcg acatggcggg cgccgtgggc cgccgtcatc cggctgtcgg ccggggaatg
     6601 gcacgatccg cagaggtcgt tggccggagc ggtcaagaga ccagtatgct ccgaggcatg
     6661 gggcgcgtgg cagcgggtgc actcgagttc gttgaagggc ggatggggca ccgcggcgtg
     6721 aagcgacgag tccagctccg ccttgtggca catgaaacag gcgctcggat catcgagccc
     6781 ctccggcgcc gcccggagca gccctttctt gtcggaagcg tgcggatcgt ggcagtccca
     6841 gcacttgccg tcggagaccg ggaaatggac gctggccttg tcgaattcgg ctttcagatc
     6901 ggcgtggcag gagtaacaga gatcgttggt tgtcgcagtc agcacaaggg tctgggcgaa
     6961 accgtggcgt ttgtggcagg actcgcagtt ctcctctttg gtgggctggt ggatgaattt
     7021 ccggctggta tagagctgct tctgtttctg gtggcagtcg tagcaggtct tgaccgccga
     7081 ggccgccggg gccaggacga gcccaagcaa tagcggtacg atcaggaacg cggcgcggga
     7141 gaatgtcttc attgccacgt gactccgcct tatttcttgt cgggtgcggg aatatactgc
     7201 gcggcgacct tgaagtcgag caacttgaag ccgagcaggt cggtgcggcg gtagtgcggc
     7261 gcatcgacat aatgaaacgc ccagctttcc tgtaccagcg tgtccgcctg ctcaacgcgg
     7321 atccgtgccg ccggattgta cagcgtgtcg gacatctgca gacccttccc ctcggtcgta
     7381 atcccaagat gcggattgaa caggatgatg cgggctttcc actcctcctc cccgatgaaa
     7441 aacggctggt cgaaccctac cgtgtcaacc tggagaaaat ccgagaagag ccggtggcgc
     7501 gtctcgatga tgtagacatc acccgaatga tcgacggttt tggtcgagtc gacatcggcc
     7561 gccgccgcgg gaagggcggt caaggccaga agaacaccaa cgagaagcgg tcgaagggcg
     7621 gacgtatggc gaagaagctt catgcgacat ttccattctg tatcgaaatt tgtgaaaaaa
     7681 gtaactaaaa ttgtcctgtt gtcaagcgca ttctgccgca tggcggcatt tgacgagctc
     7741 cggagtcaac gtttttaatt gtaccacttc gtacgatatt ctatcctggc gtataaccta
     7801 gcagggagat gtggcgccat gacctcagta gaagcgctta tcaatcggca attcatgcgg
     7861 tgggagcgcg agcaacttcg acggcaggag caggaacctc cggcagccgc cgtgccgccg
     7921 attatcacgg tgtcgcggca gacggggtcg cgcgggtcct acttcgcctc ccggctggcg
     7981 ctggccctgg actaccagcg catccatcgc gaggttatcg atgccgtctg cgcctcatcg
     8041 ggctactgcc gaagaatcgt ggagtcgctg gatgagaggt accggtcaaa tctggagttg
     8101 ctgatcgagt cggcgttgag cgggacgggg atcgaccgct cggatttcac ccgccaactc
     8161 taccgggtgg tgctgtcgat agcccggctg ggaggggttg tgctgatcgg ccggggggcc
     8221 aactttattc tggggccgcg gcacggattc cacgtgcggt tcatcgcgcc gcgcgagcgg
     8281 cgggtccgaa atctggtcgc ctacaaaggg accaccatga tcgaagcatc ggagatgatc
     8341 gacctgtcgg accggcggcg gcgcgagttc gtgcgcaaga ttttcggggc cgaaatcgat
     8401 gatccgagtt actacgacat gatcatcaac gccgactgca tcgacatcga ggagttggtg
     8461 gaggcagtcg cgatcgccta ccgggggaag tgcagcaagc tggccaacct gggcaacgac
     8521 attccgtaaa tcccggcgag gcaggcgggg gcacggcgcc catcaggcgt tcgccgaacc
     8581 gcgagacccc ggtccggtca gtccccccga cagtgcggcg gcttttgtcg atccggccat
     8641 ccaacaaaaa aggcgcctcc tgagcgggag gaaacccagg aggctcggaa gggtagaggt
     8701 aggggaacgg acttatgctt ttttgggccg cagcaggcgc atggctgcca ggccggcgcc
     8761 cagcaggatg agcgttcccg gctcggggac ctgcggcgga ggttcatcgc tgtagacctt
     8821 ggcgtctcca tgcacgtcgg gcgattcgac ggcgccgacc gactcctcgt tgtcgctctg
     8881 aggaatccag acgccgaaga catcgtcaaa agccaccgtg agcacgatga cgaatatgat
     8941 aacggcgatg acccatttca ttggattgct gtgcgtgtcg tgagaaactt tcatggcgtc
     9001 atccttcttt cgtttaccga atctcccggt tcactccaat ctctgccgct ttaatcagag
     9061 caagcaccgt gccgcagcgg gacgcggccg tcccctggcc gcccgcagtc caagccgcag
     9121 agcagcttag gggcaggatt atggacttgt ccccatagtc cggggtcggc ccgaggtcgg
     9181 cattattctg cacaaattcg cgccctcatc ggtggagaat gccgccggcg cggtctaggc
     9241 aaaaaaccgg aggagaaatc gccatggaca ccaccctggg ccgcgcgacc gcccatctgg
     9301 ccgaaccgga cgaccggcga ggcgccggcg atctcatcgc cctgattaac cgcaacatca
     9361 acccccccgc gccggtgacc gccgacgccg ccgtcattcg cgcgatgtat gtcgtctccg
     9421 acgaaatcaa cagcttcggc ggctgcttcc cctccgacga gcacgcggga ctgtgccggc
     9481 tgctggtcga ttcgccggtc atggtcggac accgccggga ccgcctcccc atcgcgcgga
     9541 atttccacgc cgaggaggtg gtgcgggacg gccgaccgtg ggtcaagtgc tggttctact
     9601 ggctgcgggc ggccgacggc gcggagaccc tccgcgagaa cattgacggc ggcatctacc
     9661 gcgagtgctc gatcagcttc accttcctct tccccgaatg ctcgatctgc ggcaaggaca
     9721 tccgcgcctg cactcaccaa ccctttgccc ggtacgcgga cggcgacggt gaagcggtct
     9781 gccatttccg ctaccgcggg atcgagcggg tgctcgagac ctcgcttgtg taccgggggg
     9841 ccctgcccgg cacccggctg accaaggacc tccgcgaccg gcatcccgtg cgaatccctg
     9901 atgagacgct gatcgaggcg ccgaccgagc tgcccgcggc ggagcgctat ctcgtggcgc
     9961 cggcgtaaga ggggattccg gtcgccgcgg cgtgcagcgg gccgggcgag ttgcggctgc
    10021 gccgcctgga caatctcgac ctccctcccg ccctcgccgg ccgctgggcc gccgaccgcc
    10081 tgccgccgtt cgatgaactc ggcggctacc tgatcggacg gcaggggaag acgcgctgcg
    10141 cgcttgcgga gacggagtgc ttcctgcgcg gcgagccctc gcccgtgacc cgtctcgagc
    10201 ttcggctcct ccccggaccg gacacgccgc ccctgagagc cgccggttcc gggcggttca
    10261 cagtcggtct gcaccgaagc gagatcggca cgctgccgca gatcgatcgc ctggcggcgc
    10321 gtctggccac gcgcgccggt gtccgcatct ggccgaccac tctctcgctg cgcgaacacg
    10381 ccggctaccg ctaccggccg cttcttgacc gaaccgcccc ggggggcggc tacaccctgc
    10441 ggcaacccga cgcaaccggc acagcgcacc tggtgttcgc cgaccgcgat gcggtgcatc
    10501 gctttcgtct ccgacagttc cagcttcagc ggctgctcca cggagcgaag tttctggccg
    10561 accgctgccc ggcgactgac gacggggcgc ccgggatcac gaccgccgag gaccaccggc
    10621 gcctgagcgg accgctgctt gagtcggtgc gggatgccga cgccttccgc ctccgcctgg
    10681 cgggccgtct ggcgggcgag tacgtgttgc ggccggtgac gatcgacggg gagcggcggc
    10741 acctgtttta ccggtgcacg tagccgcaag gaggcggcgc acctgacaaa tctgcgactc
    10801 ctgagaacca acggaggtgc atatgcccaa cgcgcatggc cccgggttca acctgctcgg
    10861 gccggtcatc aacatcgtca ccatcatcat gggcgtggtc accgcctatt tcgtgacgat
    10921 ccagtcgctc aaaatcgacc tggcggccaa agctgaacgg gcggtcgtgg aaacgctcga
    10981 caaacggctg accgcgttcg aggtgagcct gaccgaggga gcggtgacgc gcgatcagtt
    11041 ttacgaattc gcccgggaca ccgaggcgcg cctggcgcgc atcgagctgt acctggccca
    11101 acaatcggag gacgagcgtg gaaacaaatg aaaccctgat cgagcaggtc gatgacctcg
    11161 cggccgcctg gacgccggac aatctgtgcg agctcgattt cgaccgcatc cgcgacctgc
    11221 ttgggcggca ccgccggctg ctggcggaac acgagtcgct ccgggaggat ctcgccgccc
    11281 tgcggcagga ctatgtcgcc cggatcgggg gcatggcgaa agcgattgcg gcggtcaccc
    11341 gccggaccgg cgcggcgcag gcgcacgatg ccgcagcggc agttctcgag tatcttcgga
    11401 cgctcgagac gatgaccgcg gcgcagttga tcgagcagta ccgcctggcc ggcgcgcgtt
    11461 tccgcgatgc tttcccgacc agtttcggcc gcctgccgtg agtgagacgg cggcgtggcg
    11521 ccgccgcgcg cccggccgcc gctctgtccc gtgggactga ccgtcccggc cgcgcgggag
    11581 cacccgcctc ccggccaccc tgccaccaag accgatcgta acccccactc cgtgaggaga
    11641 cctatgacga ttcgaccgca gaaccttgaa gccgtcgcgc cggtgctggc gactttcgca
    11701 gtccaccaga ccggcggcgt cgacgatctc aaagacgccc acctcggtga gccggtgatg
    11761 ctgaccggca gcaacgaggc gggcccgatc agcaacggcg gccagctgct cgggaagctg
    11821 gttgcgctga cactcgccga cggcgacgac gggagccgcc tggcgaccgt gcagatcggc
    11881 ggcgtctgcc gcctgaagat ctccgccact tacccggcgg cgggcaaccg cgtggtcggc
    11941 gggaccgccg gcacggtcaa gcaggcgccg gccgtctcgg gcgacccggc cggcggcaac
    12001 atcgcgcgcg gcaccgtgct cgccgtcaac ggcgctgtcg actgcacctt tctcttgaac
    12061 tgaaggagac cgctatgacg ttcgaacagt ttcttcgctc ggcggcgctg accggcgcgc
    12121 cgcgggaatg cgaccgcgag tgggccggag gcgccgtcga cctgagccgg acggccggga
    12181 tcgagatcac ccgcgcgctc tacggcgagg cggaggcccg cggcctgagc ctgtcggagt
    12241 tgctcgagct cgacgactac gacccgagcc ccgccggggc gccgctcgac gcattcgagc
    12301 gccagctcgc gctggccggg atccgcttcg gcggcaagac tccgaccacg gtcgagcagt
    12361 tctaccagca ggcgccggcg ctccttccgg agttcatgcg ccgggagatc cgcgccgggc
    12421 aggcgatgcg ccccgaactc cggcgcctca tcgccaacac cgcgatcatc gcctccaaca
    12481 cctacacccc gttcttcatc gacacgaccg acgccgcctg cttctcgctc cggccggtgg
    12541 ccgagggcgc ggagacgccg caggtgctgg tcaccgagca gaaccacgcc gtccgcatcg
    12601 ccgactacgg cctggccctg aaagccagct acaaggcgct ccgctaccgc acggcctcgc
    12661 agttccgcgt cctcctctgg tacatcgggt tccgcctcca gacggacaag atcgggatgc
    12721 tggtggagac gattctcaac ggcgacggca acgacaacgc cgccgcggtc atcacctcgg
    12781 ccgccagcgg cgtgctggcc tacgacgacc tggtcaacct ctggtcccag ttcgcgccgt
    12841 tcgagatgaa cacgatcatc tgcggtgtca acctgatgaa ggagatcctc accctcgacg
    12901 agttcaagga cccgatggcc ggctaccgct tccagaacac gggggacctg ttcagcccgc
    12961 tgggggcgtc gctggtgcgc tgcgacgaac tggccgccga cctgattgtc ggcctcgaca
    13021 gccgcttcgc gatcgaggag gtcgtgactc agccgctgac gatcgagtac gacaagatca
    13081 tcgagcagcg ctttgaggag gccgtgatca gcgagtcggt cgcctatgcg aaggtgatcc
    13141 acggcgcggc ggtcgtgctc gataccgttc acgcttaacc accggggccg gcggcgccgg
    13201 gcgaaggggc gggctgcagc ccgcgccgga cgcccgcacc acccgccggc gcggcggacg
    13261 gccggggccg gggcgcacct ccccccccgg ccgcgcccca tgggaaagga gccacatgcg
    13321 ccagactatc tgtccttcca ccgccctcca cccgctcggc gctccgccgg ggcggaaact
    13381 gctgcgcgtt tcgtccggcc cgcacgccgg gcgggaggcg gtgctcttcc aggccgcccc
    13441 gggcgagatc gcctgcgcct gggccgaccg cccctacgcc ggctggtcgc cgcccgccgc
    13501 cgtcaccgcc caggccgagg actctccgtt cgacgcggcg atcatgccgg acgggacgat
    13561 tcacctcgtg tacaccgagg caggcaccgg gtatctcgcc gcaaggaagc tcacgctcac
    13621 cggcggagcg tgggtccccg gtcccccggt cacagtcgtc gaccggccga tcgcccgcta
    13681 cccgagcctc gcgatcctcc ccgacggcgc gctctgggtc tgttggacgg ggatcgagag
    13741 cggtacccac cggttgtttg cgaagtcctc gagcgatggc gggacaacct ggggcgccgg
    13801 tccgtctgac ccgggggagt cgctcacgct gggagcatcc tcggcctttg gccggctggc
    13861 cgtcggaccg agcgacctgt acgcggcgta caccaacggc ggcatgcagc tgtgcgtgcg
    13921 gtcgcggccg ctcggcggcg gcgcctgggg cacgagcacc aacctcgccg ccggcgagaa
    13981 cttcgacgag cacttcgacg tcgccgccgg cgtcgacggc cggctcggtg tcgtctggga
    14041 caacggcgcg ctccgctacc gggagttcga cggcgaccag tgggcgccga cggtcgtgct
    14101 cgacggcgcg gggggatcgt ggccgcaact ggtcttcatc gatttcgctc cgctggtggt
    14161 gtacctctcg gagagcggag cgggccaggc gatgccgctg tatacgaccc ggcagggcgg
    14221 cgccttcgtc gcgccggcgg cgctcgatgg ccgcgccgcc cccttcgccg ccgtcgccct
    14281 gtacgacacg gtgagcgcct cgtgggcgaa cctcaccgcc gaggctaccg ggaccgcgcc
    14341 cgccgatatc gcccatccgg tctcgggcgc cctgcttgcc cacgcgggcg acgccctcta
    14401 cctcggcatg gaggagcgct tccgcttcgc gaaagtgcgg ctgtcctccg ccggggcggg
    14461 aggcagcacc gtctgcagct actgggacgg ttccgcgtgg gcggccatca cgcccgcctc
    14521 cggcccgtac aacttcgacg tactcgagca cgacctgctc ctgtggaacg actacaacac
    14581 cgtgccggtc gactggcaga agaagccggt cgcgggcgcg gtacacttct gggtgaaaat
    14641 cgaggcgctc gccgattttg cagccgcccc ggtgggcagc cagatcaccg ccgtctcccg
    14701 cgtgactgcc ctcacggtga ggaggtgaac agtgtcgtac accaccgatg aactggtgcg
    14761 ccgccacctc gccgacaccc ggccgctggc cgaccgggtg accgaccagg cgatcgtcct
    14821 gggagaaagc ggctgggttc ttttttggcg cggaccgatc gaggcgggga cggtcgtcgt
    14881 gaaagccctc cgcggcgcgg cgccgctccg gcgcgcgctc gccctcgcgg gcggaagcgc
    14941 ggcgctggga gtcgccccgg tcgtgccggg gtcggtcgtg gccgcctccg acagctcgct
    15001 cgggacgatt tacgccgaaa acgtcgattt tgtcatcgat tatccccgcg ccgttttcta
    15061 tgccaaagcc ggcggcgcgc tggccgacga cgcgtcggta accgtctggc tgcgcgagta
    15121 cacgctgtgc gcgcccggcc tcgactacgc cctcgatgcc gaggcggggg cgctccggcg
    15181 gctcgccggc gggtccatcg cccccgtcga aaccgtccgc ctcgactaca cgccgctcta
    15241 cgcggccggc gacgacatgc tgattgcggc ggcagtggcc gaggccaacg gcctggtggg
    15301 tgcggcggtc gacccggcca gggcgttcgg cgccgatccc gccctcgagg cggcggcaac
    15361 ctgtcatgcc ctcgccgtcg tctgccgcgg atcggccgcc cgcgtgctcg ccacccgtcc
    15421 cgaacaggag agagcggccg ctgcctggac ccggctctcg gaactctacg ccgaccgcgc
    15481 cgaccgtctg ctcgcggcct tccatccgcc ggctgccggt cccgccgcgc cctcggccgt
    15541 gtgaaaggag gaaccatgct gactccgctt aacgacgtgc tggcccgccg cctgtggttt
    15601 atccccggat tcgtcgtctg gggcgtgccg gggctgagcg aaatcgactt cgtggccgcc
    15661 gagcagatcg gcggcgcgac cgcgctccgc cacggcgccg ccgtccccgg cggcggaccg
    15721 cagacggtgc tctacgccga cctcgtcgac caccggggga acgctctgcc ggcggcgctg
    15781 gcatcgccgc gcgtgtggct gcggccgcag ggcgcgacgc ctgccttcgt cgtgggcgcc
    15841 gaaacgccct cgtccttccg ggtcgcgcgc gatcccgacg gcgccgggcc ggtgaccgcc
    15901 gatctcttcg tcatcgaaat gggcgactga gatggctccg ggcgcccaga tccgcgaacg
    15961 gctcctttgc gaatggctcg cgcgcctcga attgctcatc gcggggcact tcgacgccct
    16021 gctcggattc gcccggccga aaagccggag ccgctccggc ggcgcgtccg gcccgccgcg
    16081 cagcgcccaa aacgccaaag tatcggagtt gatcaaaatg atccaactgt atgcccggct
    16141 ggccgacagc cggcccggaa ccgacgagtt ctgggagatg atcgaacgaa tccgccagga
    16201 gaaattgccg ctccggccga ccgggcagct tcccgcggcg gaggcgagcc ccaagaaagg
    16261 acgccggcca tgacgcgctc ggcggccgtg ctgctcgcgg cgctgacgcc gcttctcgcc
    16321 gaggccgcgc gcatcgacac gctgcgcgga ccgtcgcaga ttgaggacgc caccatctac
    16381 ggctacctta cctgcgacgg cgaaatcagc ggcgaggact gccggcgcta caacacgggc
    16441 aactgcctct atttctccgt gggaacccaa ggcaccggca accatcgcgc ggtgctgcgc
    16501 tttcccggat gggacggcgt cgtgccggat tcctccgtcc tggaattgtt ctgcgaactg
    16561 gaggacgacg gcgccgaccg gcggatcttc ctctatcccc tcacccgccg cttcgtggag
    16621 ggcgtcgagg gattcggcgc gggcggcatt cccgccgaca gcggcgtcac ctggaaccac
    16681 gcctggctgg ctcacggccg gggcgactcg ctctcctgga cctcgccggg aggtgatttc
    16741 accgcagccg tggcgtgcac gctgatcgcc gccgacagcg gcaaatacca gcgcgtcgcc
    16801 ggtttcaacc gcatgctcgc ctactgggat tccaccgggt ccgatcccgg cgtcatcctg
    16861 atcggagaaa acgtcttccc cgccaacacg gcgaaaaaga ccttcacctc ggccgaaggc
    16921 gccggcgacc gggcgccgcg gctgttgttg tactatcccg gcgctccggt cgccgccgga
    16981 cggcggcggc tgcagtcgac agccgccccc gcgcgctgat gatccgacga gaaaggaacg
    17041 tgcccatgaa ttatcggctt gcttttatac tcgcgctgct gctgccgggg atgtcggccg
    17101 ccgcccggat cgtccacaac cgcacgacgc ttgcgaccgg tgaggattcg ctcgccgtgg
    17161 cgttccaggt gctcgacagt ctcggcaacc cgactgaggc cgattcgatc ttcatctccg
    17221 tgatcggtcc gtcgggcggc atgacggcgg tcgactcaat gacgccggcc gacgcgcggg
    17281 tctcggtgac agcgccgggt ggtcagcccg cctatgtgtt ccactgccag gtctcggcca
    17341 tcgacggcgc cggcgcaccg ggcgtttacg cgctgacggt gatggccaaa tcgacggccc
    17401 tcgggctggc gacccagacg gcggcggcct tccagatcgt cgccgcgccg ttgtccgaac
    17461 ggctcgccct gctcgatgat accgtccggg tgaacggcgg agtgatcgac agcaaccgca
    17521 ccgagcgcgg gatggcgatt ggctcgggcg ccgtgtcggt cgtgctcatc gcctacgact
    17581 cgacccgcac ccaggtcatc cccggcgtcc gcgtcgtcat ccgcgatctc gcccagacgc
    17641 tcctgcacgg cctcgggttc accgacgtga gcggcgcagc cgccttcaat ctcgacgccg
    17701 actcggtgct cgcggtcgcg gccgctcccg gttacctctt cccgccgttc gacacgctgg
    17761 cgatcgtcgg cgcgaccatg gacacgctgt tcgggacggc ctcgtacccc gaaccgccgc
    17821 ccgcgggcgg cctgtgccgt gtctggggct gcctgtacgg cattgacgga tcgccgcagg
    17881 ccggggcgac gatctccgcg atgctcccct cgggcgtcgc gcgcttcggc ggtgcggtcg
    17941 tggcgcccag cccggtgagc accgccaccg acagcaccgg cctctttcaa ctggacctga
    18001 ttccgtcgga actgctggag agcgaggatc cccgctatga cctgacgatc tttcggcccg
    18061 acggcgtcat cctccgccag cggatcgccg tcccgaatca gccgctctgg cagttggagt
    18121 ggtaggaccg cgcccttcag cgccaagaga ggggcatggc cgccgcctgg ccgtgccctt
    18181 cccctacttc acccccgcca tgctccggcc ggcgatccag ccggtggtcc aggcgctctg
    18241 gagattgaac ccccccgtga gaccgtcgat atcgagcacc tccccggcga aatacagccc
    18301 ctgccgcaac cggctctcca tcgtccggaa atcgacctcc cccagcgcca ccccgccgca
    18361 ggtgacgaat tcctctttga aaatcccccg cccggccacg gccagatgcg tctctgtcac
    18421 ctgctcggcg aggaccgcca tgtgctcccg cgtgacggcc gcccacgcgg tctccggcgg
    18481 gatcccgact gcggccgcga tccggctcca gtagcgcgag gggagcgaga cggggctgtc
    18541 gccgtgcgcc agccgcttgc cgtgcgcctg cttgtgcgct gtcataccgc ggtagacctg
    18601 ttcggccgtg gaaccgggga cgaaattgac caccagttcc gcgcggtaac ggctctcgtg
    18661 aagcggacgc gccgcccacg cggagagccg gatcacagcc ggcccgctca gcccccaatg
    18721 ggtaatgagc atcggtccgc tctgttcgaa ccgttcccct cccactgcga gccgcaactg
    18781 cacggattcg aaactgactc ccgccagccc ctgcaggcgc aggtcgggaa cggtgaaggt
    18841 gaacagcgac ggcacggggg gaacaatggt gtggccgagc gcctgagcca gcgcgtgccc
    18901 cggccgaccg ccgccggcgg cgatcagcag gcggtcgcat tgctcgcggc tcccgtcgcc
    18961 cgcgatgaca tcgaacagcg gcgcatcgtc ggtgtccctt cccgccggcg ccgcctcgcg
    19021 cacacgcgac cccagccgta tccgcacgcc ggctcgctcc gcctcgcgca gcaggcaggc
    19081 gaccaccgcg cccgaatcat cgctcgccgg aaatactcgc ccgtccgcct ccgccttcag
    19141 gcgcaccccc cggccctcga gccaggcgat catgtcgcgc ggctggaacc gcgcgaacgg
    19201 cccgcgcagc tcgcgctccc cgcgcgggta gttcttcacc aaatccgcgg gctcgaaaca
    19261 acggtgcgtg acgttgcacc gtccgcctcc cgacaggcgg accttgtcga gcggcgcgcc
    19321 ggtcgcctcc cacacgatca cctcgccggg ccggccggtt tcggcggccg cgatcgccgc
    19381 aaagaggccg gccgcccctc cgccgacaac ccccagccgc agccggcgcc ggtcggatct
    19441 gtccttgtga ttcgccacgc gaggtagtat tgtatccgga ggaagcggtt acaagcccaa
    19501 actccgcccc gcgcggagcg gaccggtcgg tccgcggcga tggaggtcgc atgatgactg
    19561 cccaggaaag cgcccccgct ccccgaactc agccccgccg gcgggcggtt gatatcctcg
    19621 ggttgctggg atggttgatc ctcgtgttcg cggtgtcgtt cgccgcctcc cggtacatgc
    19681 cgggcgagtg gtacgcccga ctgcacaaac cgtcgtggac tcccccgaac tggatgttcc
    19741 cggtggtgtg gacgctgctg tatgcggcga tggcggcggc ggcctggctg gtgtggcggc
    19801 gcgatggttt cgccggggcg cgggcggcgc tcgtcctctt cctggcgcaa ctggcgctca
    19861 acggcgtctg gacctacctc tttttcggcc ggcagtgggt ggggttggcg ctggtcgaca
    19921 tcctggcgct gctcgccgcc gtgatcatca ccatggtcct cttccgcaaa cacgatcgcc
    19981 gggccgggta tctgctcgcc ccctatatcg tctggctggc ctacgcctcg acccttaatc
    20041 tcggcgttct cgttctcaac tgagcggcgg gcggcccgag ggataacaag ggtgcggcgg
    20101 cggcatcgct gccgccgcct gccctatggc cacggcgacg ccgcagtcac ttcagaagca
    20161 gcatcttcct ggtctccacg aaatcggccg ccgtggcgcg gtagaggtag acgcctgacg
    20221 catacgccga ggcgtcccac tcgaccgtca ccgatccggg cgccgaggcg ccgtggaatt
    20281 ccctgatgag ctggcccagc gtgttgaaga tctcgatccg ccactcggcg gcgacgggca
    20341 gatcgaactg aatggtcgtc gccgggttga acgggttcgg gaagttctgg tgcagggcga
    20401 aggtcgaggg ggccgtgggc tcgaccttcg cgttggggtc atccggaatg tcgtcatcca
    20461 cattcccgct tccgcacgac gagcagccgg ttccggtgga gctgcacttc agagtgatgg
    20521 tggagccgtt cgtggcggcg tacatcggac cgccggccgc gtagtcccac accttgccgt
    20581 tcgtgaccga gatcccgatg ccgtagggtt ttccgcgaag gcagggagat ttcagcccga
    20641 tgcggagccg gcagactttc acgggatcgc ccgtcggccc gacgcccgcg aacagggaat
    20701 tggtctggat gtcgtaagtg tggtagttga cgaaagtggg aatgaccccg gtcatccggt
    20761 cgatgatccc gtagatgcac gcccaggtgg gcaccgtgat gcgcacggag aaggaatcga
    20821 tggcgcaggt cccgcgaacg tagaagtcca cctcgccgca ggccgtggaa gaactgccgc
    20881 cgccggcgag gagttggccc atgagaacct ccaccgagtc aaacgccccg cccgaccagg
    20941 cgtccaggat gccgttgacg gaatccgccc ggaggcaccc gaccgtgtcg gggacgaccg
    21001 ttcggccgtg gccgatgccg aaatcgtgga taaatttcgg acggaggtag cggttgttcc
    21061 agctgggcgt gaatccggcc gtgtcgaacc gcaccttgag cgtgaccaga ctgtcgaagt
    21121 cgccgccgcc cggcaggtag ttggcgcgcg cggcgtccca ggtctggtag accgccgtca
    21181 ggtcgccctg gctcagatcg aggaacttca ccgcgggcgc atagtggtcg atccccgcga
    21241 agtcgaggtt gggtgtccaa tcgatgttca tggtcaggcc ggtgtcggat tgcgacccca
    21301 tgcccgcggc gaaggtgctg cgcatgagca cgccgaattt cagcagctgc ccgtcgtcct
    21361 gcatcgtcac cggctcgccc aggtagcgcg cccggtaggc ggccgtgtcg gccatcgcaa
    21421 tcgtgaccga cgggtcgctc acagccgccg gatcgatcga accggagcac tcgctgaccc
    21481 acaccgtgag ccagccgggt acggcgttca gcaccgtcga ggtgttgccg agcggacacc
    21541 tcggcgccag cgtgaggtga acgagcggga agtggtggcc ctcatccacg ccgctgaaat
    21601 tcagcgcggt gatgacgatc gtatcgctcc gcagcgtgac ggtgccgccg aagtagggcg
    21661 gcatctcaaa cccccggaat tccagcttgg cggtgtcgaa cgtgtacgcc tgccagacga
    21721 ggctgaggcg aaagtcggtg gtcgcgtact cggtgagtac aaactcctcg ccgacctcac
    21781 ccgtcaccgc ggtatcggcg atttccagcg tgatatggta tgccgagtcg ggcatccggt
    21841 cgaccgagcc gtcgtcccac ccctcgcttc cgggcgagta gataatgccg ttgacagtca
    21901 ccgagttgtc catcccgttg cgagagaagt tcacgccgcc gggcagcgaa tcctggggcc
    21961 agcactcgtg gatgaacctc aggcggccat actcgcgcgt gccggccgag gccgtatgca
    22021 gcgggctcgt tgtgtccagc gtcatctgca tctgcacgac tccatgcggg ctttcctgag
    22081 catcgaagta ggcgtaccag ccggggtcgg cctccgctgc cagaaagctc atccgcgtgg
    22141 tgtcgtacag cacttttacg tggatctcgg tgatggcgcc gttgagctgc tcgaagaggg
    22201 agtcaaaacc gatgcccaac gataccgttg cgacctgggc cgccgagtcg ctcaccgtac
    22261 ccgtcacgta ggcgaggccg tcgccgaggg caaaggtcac atggtccgac gtttcggaca
    22321 tgacctcgga gaacgccggc ccggccgccg acgcggccag caggaagccg gcgaaaaacc
    22381 ctctcataat gcacgtgtgc gtcaatgcat gcatagaatc ctccctggaa gttttgaaac
    22441 gtgtgcgcgt agtcgtggac gaacagattt cctgtgcccg gtctcacctc ccttcgccgc
    22501 accggatgat gcccggcacc ccgattgccc ggcgcgcggc gagccccgcg cggcgcgcct
    22561 ccgagtcatc atcacgctat tcgagtaata accgtgtccc cgcccccgcc ctgcgtctcc
    22621 ggacctcccg gttgagccgg caaaattcta cacgccgcgg ggccaggctg tcaagagatt
    22681 ttctgggcgg tccggcggct cccgccgggc atagaaaggc ggcgaaaccc cggggttccg
    22741 ccgccggctg cagcctccct cgaagaaggg gtcatgtttg gaagaggtat tgcagtttca
    22801 ggaaaaaccg ccgcgacgac atccgccact ggtccggttc atcagtcccg gccgcgagat
    22861 tctggtagtc gtaggtcgtc ccgacataga agacggaaaa cgggctgagc tggtaggaga
    22921 gaagcgggtc gatatcccac cgctccgacc cgtcgttgta ctgtaccacc agccgcagcg
    22981 acagctcccg ggtggcctgg aactggacgc gggtccgggt gatgtgctgc tcgtacagga
    23041 gttcgtcggt ggcgacgtgg cggctgcggg tgaaggtgta gctcggttcg atcatcagcc
    23101 ggtcgagcgg cttgaggtcg acctcgatcc ccccggtcac ttgatttcct gtggccgact
    23161 gccgtacggc cacgtcgcgg ccgaacgaca ggtcgccgtt gaacgcgagc gcattgctga
    23221 tcacgccgcc ggcgccgatc cgcacggcgt agagaccgtc gaactgcacg cccccccaat
    23281 tctcgtcacc ggccgtgtaa gtgagcgagg cataactctg ccagaagcgg aaattcgcct
    23341 ccagcccggc cgtgccgtgc gtccatttga tcctgccgtc gtagttccag cgccggtcga
    23401 catagatatt cggcgccagg cgctcaatca gacggccctc gccgtagaaa aggtagtacg
    23461 cgttgacgaa cgcgttccgg tagtcgttcc agggatcgta gccggtctgg gtccgatagg
    23521 tcggatccac ctggtcgtag ttgagacgga aattgaggtg ccgcccctgg cggcggaact
    23581 gggtgatgag ggcgttgccg gagaacgact cgccgtcggt gagcaccgtg tgtttgccgt
    23641 tgtcgaaagt catgccctcg aagcgctcgg gctgcccgaa caattcgggc cggtcgggtt
    23701 cgccggtgta gctggcgacc acctgcccga cccagctgta gttgcgggag agccggatgt
    23761 ccgcatcgag gctcgccacc gtcccgtacc ccccccagtc gtagcggcgg tcggtgagga
    23821 tgaaaccgcc gcgcgagtcc tgcccgaacg agagcgagcc gcgcaggacg ttgacggtgc
    23881 tggcgccggc gcggaggctc cccccgctct cttccagcgg cacggtgtag ggcgagttct
    23941 catcccgggc gctgagaaat ccgagccgcc accgcccggc ccggccggtg agtttcgccg
    24001 cgtaccgggg atcccagatc gtgcgcgagg aaaacgagtt gaagagcgtc tggaaaatgt
    24061 ccgccccctc ctggaaaaat ggccgccgct cgggatagag caagtcgatg gtcgaattga
    24121 tgtcgatctg ggcggcgtcc gactcgatct gactgaaatc cggattccag gtcgcctcca
    24181 ccgtcatccc ggaggtcacc gcgtacttcc cggccagcga catctccccc tcgaccttct
    24241 cgttgtcgaa cgggtacccc tcggcggcga cgatctgtcc cgactggttg gccaccgcgg
    24301 tggcgagcaa ttcgactccc ttcccgggcg ccacgtcggc gatcccctca accgtccccc
    24361 actggcaggg ccagcactgc tcgctccggt tgtaggccgc ccaggagtac tggaaggacg
    24421 acgcacgcgg gcggttgcgc cagaagtcca cccgccacga ctgctcggcc ccctggggaa
    24481 agcgcagccc ggaaaatggg atcgcgatct ccacccggta accctccggg gtgacctgcg
    24541 ccgccgaccg ccacaccagg tcgaaactcg cgtcctcgcc gaccgtcggc gtccagagga
    24601 tgtccttctg cacaccatag ggattgacga agaattcata ggccgaggcg gcgttgccgt
    24661 acgtgtcgat cagcacgatc gcgcagtcgt cgccgctgta tgcgtcgcgc cgggtcatcg
    24721 tggcccggag cgcccgggga tcatcatcgc agatgaaccc gacatagaga ttggccttgt
    24781 cgtaggtgac gagcacctcc gtgcgcaccg ccggcggcgt catgtccccg ggcgagcgct
    24841 cgacgaaacg gtcggtgcgc gccgcgtttc tccacccctc gtcgcccagg tccccgtcga
    24901 cctcgatcct cccagccgcc gggcgcagcg tgatcgacgg ccggtacacc ggcgtgaaat
    24961 ccgaggccgg cagcatcgcg gtcgaaccgc gagcctgggg cgcaagcccg agcaaaacag
    25021 ccgccgacag agacacaagc aaaccccggt tcactgcgcg cacattcata caccttcccc
    25081 tttcccattc gtgcaagatg ttcctggttg tacgcaaccg tccgcctggc cgctgcctcc
    25141 gcaccgtggg cggaacagga ccaggatctc cggggatcgg ttccttttga gatttcatac
    25201 gggacggatg cgcaagcggt ttcgttccgc ttgacaaagc gcgtcgcaat gtctgcggcg
    25261 cgggcaccgt tgaccaaagc aactacccgg ctcggtgcgg ctatgctgcg agatctgcaa
    25321 accgacccga attccacggg acgatcctca aaatctcccg taggtcagga tttttgtgat
    25381 cctgacattc tcgccatcga tagaagcagg cccgtcagga ccacaagggt cccggcctgc
    25441 gaaatcccgt tcgcgggcgt atccggcgga atcggacgcg gccctgccga gcgcccccca
    25501 tataaacgag aagcccggtc ggatgaccgg gctcccgaga gaaaagcttc gcaagcgcga
    25561 ctacttcgac ttgaccacca caaatgccgg gtagatctcg agccgcttgt cggcgcggat
    25621 cgcctcttca cccaggcggt tcttgatttc ctcgaactgc gccaccagca tgagcgagtt
    25681 gctcgggctg gtggtcgtgg tgtccacaac caggttctcg accggttggt cttcgatcga
    25741 ggagacgaag acagtcacga gccggccttt gccgggcgcc agcacgttgg tgctcggccc
    25801 catgttcgcg accatgccga gcgtcacgca ctgctgcatc gtatccgggc ggaagccgcg
    25861 gtaggcgaaa tgatcgaccc ggccgccggt gtagacggcg gagtcggcca cgatccgcac
    25921 cgtcccgctc gagagtttca gcggcacggc gaggccctca acattctcgt cgttggtggc
    25981 gctgattgtg atcgaccaat tctgcggatc gatccgggag acctcggcgt acacggtatc
    26041 gaggatgccg aaattatccg gcttggcggc cggtttttgc ggttcctcgg cggcgccgag
    26101 cgacgccagc agaagaatcc caagggcgga cagcgttaac aatctggtga tgtgcatctt
    26161 tcctccaggc tgaattcggt cggaccgtcc gccactgcgg cgggcggcct attctctttt
    26221 acaattcaga ggtcggttcg atctcagtca ggccggcttt cagcgcggtc aggagcgaat
    26281 cggcaacctg ctgttgccca aggcgttccc ggcggatcgc ggcggcccgg acgagcgcct
    26341 ggcggccgat ttcatgccgc ccatatttcc ggtacaagtc ctccaacgtc ccggccgcct
    26401 ccgcccagtt gcccaggcgg ctgtccaact cggcctgaaa actcagggcc cgggcctcca
    26461 tcaggccttc ctggctgtag cgagcgacct gggcgtaata ctcccgggca tcccgatacc
    26521 accggtcggc ctggtccttg cgccccaact gctcgtagcg ccgcgccacg tagagaaacg
    26581 cccccatcgc ctcgtcggaa ccccggtacc cctcgatcag agcgtggtac tcgacctcgg
    26641 cccgatccca gttgtcttcc agttcgaagg tgcgggccat ggccagctgg gccgggggag
    26701 tcatggccca gtagcgggga tgatcacgct tgagggccac caggatctgg cgggcatcgc
    26761 cgtagcgctt ttgctccatc cggatcagcg ccttgcggaa gagcaaatcc gggtagtagg
    26821 ccgtgtcgga gggccccagc cgggccagga gagtgtcgta catggcggcc gcgcgggtga
    26881 aatcgcggaa ctgaaaaccg acgatctcgg ctatccgggc gcggactgcc ggatacgcct
    26941 gcgaggccgt gtccgagagc tgctccaact gcgcgatctc tttctgcccc tgcccggttt
    27001 cgcggtacaa tccggccagg gccgcccggc aggacgcctg caggagccgg tcggaagtct
    27061 tcgcggcgag gtcggtgtag tagctcaccg cccgctccag atgataggca gcttccaccg
    27121 aatcccccac ttggcgagcg acggcgaaga tgtgggcggg caggtcgaac agcggcttga
    27181 cgatttcgcc gcggctgtcg accggcggga agaacgtttc cagcgccgag gtgtacatcg
    27241 ccagcgcact gtcccaccgg ccggaggcct gcagggtgcg cccgaggttg acctgggtca
    27301 ccatgagttc ttcccgcgcc agggcggact gagagaggag acgggtgaga acggcgatcg
    27361 actcctcgta ccgccgctgg ttgtagagca ggcggttgag ttcgttgccg gcctgaaacg
    27421 cgatctgctg cagctcccgg tactcgaccg ggttggcctg ctcgctgacc gagtcgagcg
    27481 cggccagggc gaactccgca gccgcggaga gcttctcccc ggcccgccga accagctcgg
    27541 gcggagcctc gcggggattc acctgggcct gcgcgatgat cttctcggca tcgaagagct
    27601 tcttctccgc ctggaagcgc aggcgcaggg cggggttgtc ggcgcagccg aaagcggcgg
    27661 cgaacccggc ggcgagaatg agaattcgcg agattcttcc catttgcgtt attatcggca
    27721 gtctgacaaa ccgttgccac agagtccgag gcaaatatac gggcggggga aacgggaaca
    27781 agtcaaaaaa cagcggcgcc tgcggccacc aaagcgcacg ggccgcccga ataactccgg
    27841 gcggcccgcc tgcaaatctt atgggtcaag ggatcatgcg tcgcggcgac gcaggcggcg
    27901 gagcatcacc cccgacagcc ccagtcccag aaggagaacg gtcgccggct ccggaaccat
    27961 cgccaccgac ttctccatca ggtcattgcc gcaacccaga gtgacgtgaa aatcgagcga
    28021 ctggaaatcc ccgagcagcg aacgggcaaa cctgaactcc cacacgtacg tgtcgccgtc
    28081 acccggcatc agatgattga gctcgtagtc atcccagaaa ctgagcatcg tattcacctg
    28141 gccgaggtaa gtgccgctgg tcatccggta gtcgccggcc aagggggcga tgggcgtgcc
    28201 gtagtacgaa ccggggatat tcgggatacc ggtgcggccg gttactcgat acaggccgtt
    28261 cgagccgccg ttgaccagat caatggcata ctggtaaccg ccgccattgg tgccgatgaa
    28321 caggtcgccc aggttgtagt tgcggcccca gccggtcgaa taggcggagt aaccgaacga
    28381 gttggtcagg gcgatgtaca catagtcctg atccaccgct actttcaaac cctcgaggtc
    28441 gaactcctcg cccccctcgc cgagggtacc gggcgacggc aggttgccta ttccgccggg
    28501 gtaattgatc ggcgaccagt tgttgttctc gtcgaacggc gagtcgccaa aatcatagta
    28561 gtgaaaattg aaggccgagg cgggagtcgc cagacaaaag ccaatgcaca gcacaagaac
    28621 aaggatccgc atggttgccc tttcgcagtt gttctacttc tatctgttca aagttttagc
    28681 aactgcaatg ccgttggctt tccccctgct cagtgtgcat cataagatat tgtgacaccg
    28741 ttacttatac gacagagcgg agactgtact ccgtggcccg gcctggcttt ccctgcaaca
    28801 agttgcacaa caacgacaat ccgtgtcacc acgacctgcg ggaaatagaa aaggccaccc
    28861 tgatccgggt ggccccagaa ttctcgattc gattgaatga agctcggtta agctttgcgg
    28921 cggcgaagca acgccaagcc cgcgataccc agaccgacaa gcacaagagt ggccggctca
    28981 ggaacggtcg tgaccgtctt ggggagcgtg gtgttgcccg gtcgaatgat caccccgccg
    29041 gtcccgtcgg taccggtcgc ctggcttccc gacgtccgag ggtcgccggt aaaatacgcc
    29101 tgcccggtcg tgccgactga accgtcggcg acggtgatct gggcgcccca atcgatcgaa
    29161 gacacatcga ccagccgtgg agcgctcagc ggcaaagcaa ccgcttgagc tgcacacacc
    29221 aaggtgaccg ccaaaaggac gatgatcgct ttcatccgaa acccattttt gtttagggta
    29281 aagatctcct gtgcaatttt ccagcaattt tcgtgccacc tgacccaatt cgacagcatt
    29341 ctgctatgat cttgtcaatc ataatattaa ctgtttgacc ttatcgtccc gagaacatga
    29401 cgtcttcctg tctgcacaca cacttgtgca cgcatctgcg ctgtttctgc aaaccacttg
    29461 cagcgtcttc tctcacctgt aatcccgctc ggctcacaat cataccagga ccgcgcctca
    29521 aatccggcct gcgcgtctgg caaagagctc acgccaaaat tcggaggagc ctgctatgtg
    29581 tgccagtcgg aggaactcgg gtcagttggc cgccggggtg cttgtgccgc ccccatccgc
    29641 tctgagcacc ggcggtcaaa tcgaccgcca actgcgcttg agcacatcag acctccagcg
    29701 gggccgctat cgcatactgc tgtaccgctt tctcgccgat tcgatcccgg cggtggccgc
    29761 ctgtgtcggg acctgggttc gtttggccgc cgcgccgggc cggtttctgg tcgacggcga
    29821 tgtctcgcct tctgtccgcc gccaagtgga agagcggctg gagcgcctga gcgcgcacgt
    29881 gtaccgttcg gtcgggggga cgcgggcggg cggcgccgat ctcccggtcg acattgcagc
    29941 cgggttgttt cgcgacggtt tcttcggcgg cttcgtgacc gtggacgcaa ccgggaccgg
    30001 tgtggaccgg ttcatcccgg tcgatgccgc acgggtgaat cttacggtgg aggacggccg
    30061 ccggcaattc cgctatgccg ccgaccacgg cgacctcgat ctcgatcgtc ccgacttcta
    30121 ctacatcccc ctctccggcg gcgtcaacgc cccgctgggg cgctcggttc tgcaggcagt
    30181 gccgtttgtc gcctatatcg aacagcagct ggtcgatgac atgcggcgct ccagtcacaa
    30241 cgcggggttt caccgcctcc atgtgaaggt gacgcccccg gagcgtctcg ccggcgaatc
    30301 cgacacggcg tacgtgcagc ggatcaacgg ttacttcgac gagacggtcg ggatgatccg
    30361 gtcgtgcgcg atcgacgaga acccggtcac ctgggacaac gtcgccatcg actatatcgg
    30421 tccggacgcc acccggagcg tgactaacag ttggtttttc agccaccgat cgatgatcga
    30481 ggagatctgc gcggggaccg gcctcgcgcc gttcctgctc ggctattcct acggcgccac
    30541 gacttcgtgg tcggggttca agttcgacct ggtcatgcgg caggtgcgct cggtgcaggc
    30601 ggagatgcgc cgcctgatcg agtggatcgc cgcgatcgac ctcgccctgg ccggcttctc
    30661 cgcgcgcgtg cgctgggaat tcgacaacac cttcgcctac caggccggcg agcgggccgg
    30721 ggtcgaatcg acccggatca acgatctcat ccggctgtac gaggtgggat tgatcgaccg
    30781 ggccacggcc gcggcgaaag ccgccgggct cgtgtgagcc ggcccggccg cgccgcgcgc
    30841 cccgcggccg cacccggccg atttgagatt accggaagga cgctccatga acgcgcttgc
    30901 gaccgactgt gagagcatcg actggcggcg cgcgctcgag gatccggcgt acttcgcgca
    30961 ggccgtgctc ggcgtgacgc tccacgccgg acagcggcag tggctggaga acgcgctggc
    31021 ccgggagaac gtgctggtga ccggcaaccg gtggggaaag tcattcgtct cggccgtgaa
    31081 actcctccgc cacgccctgt tccggattcg cggctcgctg gcgtttgacg ggtcggggcg
    31141 gtaccgcgcg gtcgcggcgt cgatcacgca ggaccaggcc aacctcctgt tcaaccaggt
    31201 ggtccgactg gcccgccagt cccccgtgct gcgcccccgg atcgcctcgc tcgtccgctc
    31261 cccctacccc atgctgaccc tcggcaacgg cgcggtaatc gaggcccggt ccacccagaa
    31321 ccggggcgag tacctgctcg ggaacgacta cgacctgttt gtctttgatg aagtggcgtt
    31381 cgaggcggac cctgagtatg ttgtcgagga ggtactcctc atgcgcctgg ccgaccggga
    31441 ggggatgctc gatctggtct cgacgcccaa tgggaagaac tggttctacc ggcgggtgag
    31501 ggagctggca cagggggcgc ggccggggta tttccagtcc ggcgacagtc gcgccaatcc
    31561 ctacatctcc cgagctttcc tcgacgagcg ggtgcggcat ttcaccgcgc ggcggctcgc
    31621 gcagaatatc gaaggtcagt ttgtcgacag cggcggcgag gtgatccgcg gaagcgatat
    31681 cgatgccgcg ctggccggaa tctccgggtc ggaggaggcc ggcggggcgg cctccgggga
    31741 gatccgctat ctctccgggt gggatctcgc gcgcaagcgg acggcgacgg tcgggatcac
    31801 ggtcgcggcg gcggacggtc cggcccgggt ggtcgccctc gagcggctga aggagacgga
    31861 ctgggcgacg gtgatcgcga gaatcagggc gcgccaggag cagtaccccg gccaactggt
    31921 gatcgacgcc accgggctgg gggatccgat cgtcgaggcc ctgcgcgagt accgtccgac
    31981 cgcggtgatc ttcaccggcc cgcgcaaagc ggaactgctc accaacgtcg agctgtttca
    32041 cgcccggcgg cggatcgtgt acgaacggtg ggagctgccc gacggtccgg ggcggacgtg
    32101 gtcgctggag gatgaactgc ggagggcgcg ctgggacgac aactgcgagt gcgacagcct
    32161 catggcgctg gccctggcat tgtggccgct ccgcacgcgc ccggccccgg tgatcgcccc
    32221 gcgcctcggg cgggcgtgag accggcgacc ggcaatcgcc tacagggagc cggaaggtgc
    32281 gtccggatcc tccccggccc gggcgagttt cacccgcagc gtgaccgtct gcttccggcg
    32341 gatgaacttc ttcagctccg cgctgagcgc cgcaggcagc agacccttcg cgtaaccgtc
    32401 cttcatcagc gccgcgctgt ccagcttgaa atactcgatc aggccggcgt cgcgcgccag
    32461 cgccgtcaga ctcgcgaaat cggtgctgct gtcggtcttg gtgacaaact tctcctcctc
    32521 cgtgatcccc accgcgacct ctcccccttc acctgagaac ttggtcatct ggagctcccc
    32581 ggccagccgc acgaggtcgg cccgcagggc ggccatttcg gcctcgagct ctttgaccgc
    32641 cgcgtagcgc gcgatatact cggtcgcttt ggcgtaggcc cgccgcagag ctccgtcccg
    32701 gtccgcctcg ccggcctcga tcagcagctt gtgccgcttg gccggacaga gcgcggcata
    32761 ctcgcaccag ttgcacagcg acgattcgcg cgtcggaaac tggctgagac ggtcggcctc
    32821 gtggatcgcc aggatctgct ggcggatgtt ttcgacgagc agttcgagat catcggggcg
    32881 gaaccggtgg gcgacgacct cgtccatgcg caggcagtac tgggccgcct cgatgtcgcg
    32941 gtactgcggg tagttcgcca tcaccgccaa ctggtagagg cccatctggt ggaaataatc
    33001 cgggtcgtgc gggcgggaaa ggttcttgcc ggtcttgtag tcgcagatct ccaccgtgcc
    33061 gtcgtcccgc cgccacaacc ggtcgatgcg ggcgctcagc cggtagggcg tgccggggag
    33121 ggtgaagtgg aggtggagct ccgcgcccag cagggcgccg ggccggaacg gccggtagcg
    33181 ctcgtggtgg cgggcgagga tttcacggcc gatgcggatg taatcgtcga tgcccatgtg
    33241 ctcggtcaca accgccagga gagcgcggtc gacctcgcgc caggcccgct cgtagatctc
    33301 gagggcggcc tcgagcgaca gctccacgcc gtcggcgccg cgctcgtaga gcgcctggag
    33361 gacggtgtgg acggcggttc ccaggtacgc ttcggcggag atccgtttcg ggatttccgg
    33421 ccgctcgagg taccggaact gaaatttgcg ccggcactgc gcaaaagtct ggagcgcgct
    33481 gtggctgaag gtcgtgagca tggacacgca agcctccctc gtgtgacgcc ggctgagcta
    33541 ctgcggcgga ccggcctccc gcgccggcgg aggggagtac gattcgatca cgcctttgag
    33601 aaatcccact ccgaggccga agtggatggt tgggaaaatc aacggtccgt acagcaggca
    33661 gttgagattg cgatgccgca gcgcgagact gagggaatag aaaatgacgg tgaggaggta
    33721 gagcgccgcg acaatctgga agagcttcat caccgcgagc gagagaaacc cgaggacgaa
    33781 gaggacgatg cagaggacga cgccggcggc ggccagccag tgggtgggcg cccagagtcc
    33841 gtgcttgcgc gacatgcgga aacgcccgag accgtagcgg accatctgcc gccacaaggc
    33901 cggcagagtc gcgcgcgggt agtagtagac tctcagtgcg gggttgagcc aggctttcag
    33961 tttcgcccgg tggacgcgga agttgagttc gatgtcctcg ccggcgtcga agttctcgtc
    34021 gaacagcccg acccggtcga aggtcgcgcg cgtccacatc gccccggagg aggtcgggtc
    34081 gacctccccc tcgtagtcgg cgtagatttc cgatccgggt ttgtggccga gcgccgagcc
    34141 gcggcagagc gcgatcgccg tctgcaaatc gttgaggtcg ggcggagtga gcggctgcgg
    34201 ccggcagaga cagtcggcgc cggtgcggtc gaaggtttcg acgacgtcgc gcaggagggt
    34261 cttgctcgga atgtacacgt gaccgtcgat gacgacgatc cgcggcgcgg tgctgttgcg
    34321 gatgccgatg ttgcggcccg aggaagggcg gcgcaccggg ttgtccagca gccgcagggc
    34381 gccgaaccga gtcttgtagg actcgacgat ttcgcgggtg cggtcggtcg acccgccgtc
    34441 ggccaccacc acctcgaggc ggtccatggg aaaatcctga aggtacaact gctcgagggt
    34501 ctgcccgacg tacttctcct cgttgcggac gggcatgatc accgccaggt caaatttcga
    34561 cggtttgctc tcgggcatag ggaccgttgg ctcctgattt caggtttacc cggacgccgg
    34621 cgccggaccc gaccggcgcg cgggggcaat atacgcacgc gcggccggtc caaacaaggc
    34681 aaaaccgggg agtgcgcggg ggcggggccg cgcccgctca cccgtcccga tccgggcggt
    34741 actcgctgat cacgactttc ccctcctcca gcgtgacctt gcagcgtttg tggatgtcgg
    34801 tgaattcgcg gtagatgacg ccgaaatggg cggagacacc gtggtagagg cggtcctgga
    34861 cgatgatctg cgcgcccgcg acctcgcgca ggcgctcctc gagccggtag cggacgtcgg
    34921 cgaggcgttc gagctcgccc ggggcggcgg ccacgaattc ctccagtcgg cccagggcct
    34981 cggcccgctg gggatcaagc agaccctccg cctgcagccg gtacatggtc agaagggtgg
    35041 cccgaatgcg cgcgaggtct ttctcgatgc gcgcgatttc agcacccgtg cagcggtact
    35101 cgcgcatgag ctcggcgtcg tagccgacgc ggaggatggt ggcggtgccg gcgtcgttgc
    35161 cggcggtggc gacgcggatt tcgcggccgg cgttggattc gccgccgatg atttttcctt
    35221 tgcgggcgcg gacgatgatg cgctcggccg ccgtgacctg gcagttgagg agttcgtcgc
    35281 cgacgagaac gtcgttgccg gagatgatgt tgaccccctc ggcgtacttg accagcacgt
    35341 cgccgtcggc cttgatcatc ccctccccct tgccgtaggc gccccccttg atgaggatgt
    35401 tgccctcgca tcggatggtg cagtcggcga cgttgccgcc gacttcgagg ttgccgccga
    35461 cattgagggt gaagccgggc cggacgtcgc cgacgacgcg caccgagccg atgcagttga
    35521 tgttgccgat gctcatgtcg acgtccccct cgatgcgggt gacgtcgttg acggagacgg
    35581 tgccgcgggt gaagacgatg gcgccgctgg cggccgccac cagcgagagg ccgtccggct
    35641 cgacgcgcgt gttcttcccc tggcggaagt tgaaatcgcg cccgcgaatg ccggggatca
    35701 gacggccgtc caccccgcac ccgtcctcgc cgtcgcctgg cggaatcttg cgcaccagca
    35761 cggtcccctc gcggacgttc tggatgaaat tgatcgagcg gtagtcgatg cgcccgtcgc
    35821 cggattcctc gggcgtgtgc tgggtctcgg gttcgaaggc gtactcgaag gcggcgtcct
    35881 ttccgcgccg ggggggcgtg ccggtggcga ttttggtcgg gacgtcgtag agccgctggt
    35941 cgaggacgcg cttgatggcg tcccagtcga tgccgtgggt cacgccggca cggacgatct
    36001 cctcccggac gtcatcgagg gtgacctccc cgccgaggtg ttccgggtcg ctcacggcca
    36061 tcatggcgga catgttgtcg cgcgtgatga cgacccgcac gagtttcggt ttgggacgct
    36121 ggacccgggt ttccggcgag gtctcggtca tgggtgcact ctcaggccga ggagttcgag
    36181 gcggccagga tgcgccagta ggcgcgctcg accaccaggg cgatttcgta gctcttgaac
    36241 ggtttggtga tgtattcgtc ggcccccagc aagagggcat ccttgacggt gtaggtgtcg
    36301 ccgtaggcgg tcatgatgat cacgccagtg cgggggtgtt cccgcttgat gatcttgagc
    36361 aggtcgaacc cgttcatccc gggcatcttc atgtcggaga tgacgatgtc gcagggttgg
    36421 ctccggagca gggccgccgc cgtctcgcca tccggcgccg tcgccactcg gtacccttcg
    36481 cgggagagga tcttctccag caggagccgc atcatggcct catcgtcaac tacaagaatc
    36541 gagaccgcac ttttcatggg cctggtgtcc tccgtgcaat tgtcgttcgc cttttgggtg
    36601 ccggtctccc cggcgcccgg tcaccggctg agcgcggacg agatttcgga aatgaaatct
    36661 tccagctgca ccggtttgta cattacgcgg caggcgccca gcaggccggc tttgtgcacc
    36721 agcttgtcgg tggcgttgcc cgtgatcacg accaccggcg tccgggggcg ccgcttcttg
    36781 atctcggaga gaacctccag gcccgacatt tcgggcatga tgaggtcgac cgtgatcagg
    36841 tcgtaagggg tggcctcgac tttcgccaaa gcctcgagcc cgctgtcggc caggtcgacg
    36901 cggaaagcgt cggtcgttcc gcagaaatcg cggaagacgt cgcgcaccca cttctcgtcg
    36961 tccacgatga gcacgcggaa gggctcgccg cgttcctgag ccaggcgggt cagtttctgc
    37021 atgtcgctgt cgagcatcgg catcatcccg gttggggttt ggcagtgtcg tttgcgccgc
    37081 tctttgcccg gtttatcggc aaatccggac aagtcttgta ccgcatcggg caaggggcgc
    37141 tgcgcgttat cctttcgtgt cgacccgtcc ctcctcggcc agagcgtcca tgatgcgggt
    37201 gcactgctcc aggatggcgc ggagacggcc ctcgatcggg tcgctccccg gcggccgggt
    37261 ggcgaggtcg ttgaccatcg cctcgaggtt ccgggcggcg ttgatgatat ccgccagttc
    37321 gtgcaccacc ttgaccgtgc cattgaccag cttgatgaag tcgggttcca ggcggctgag
    37381 ggtcgccgac cgccctttgg cgcgggcgag gtagatggcg aggttgaggg ccagcacctt
    37441 cacctcttcg ttcagctcat cgacccgttc gatgatggcc gggtcgatgg gccggaactc
    37501 atcggttcgg gatttcgtca tggcatcccc acttgctgcc cggcgagggg catttcgagc
    37561 accaggagcg gggctccgtt gtcggtcgtg ctcagtcggt aatcgccgcc gtggtagcga
    37621 attgtctcgc tcgcgaccag cacctgcagc cggagggccg ccgtttggcg ggcgaaaatc
    37681 tggtcgagca tgcgcaggag gcgttcggtg cgctccggcc gcgtctggaa cgccaggctg
    37741 agccgcgccc ggccgtcggc ccgctccgtg cgcacgtcga tgcgggcttc gtccgccgtc
    37801 tcttcgacga tcatctggaa caactggtag aacgcattgc cgagctggtc cgggttgccg
    37861 tataccgaga gccgctcggt ggccgccgac aggctcaggc cgctcggccg gtgttcggtc
    37921 tgaccgcaga gacgggcgat cacgttggcc gcgaggacgg agaaatcgaa gacctggttt
    37981 tggcggctgg aggccgaggc gaattcgatc acctggttca agagcgcgtc ggcccgccgc
    38041 acttcgctgg cgataatgtt gagatactcg gcgccgcact cgctgcggtg gctctcacga
    38101 agcaggttgg tgaagccttc gataatggtg agcgggttgc gcagttcatc ggccacggcg
    38161 gcggtgagct cgcccatgac cgacatcttc tcagcccgga tgacctgctc ctgggactgt
    38221 tcaaggcggc ggtaggtgcg ttcgagctcg cgggcgcgct ggcgctcgcg gtcgtagagg
    38281 cgggcgcgct ggatcgccac ggccgcctgg tcggccagca ctttgagcag ccgcaccgcg
    38341 tcgtcgccga tcggcgcgcc cgtgatcgcg ttgtcggcca ccaccaggcc ttggcacgtc
    38401 cctttgctca tgagcggcac gagggcggcc cgggtcgtcc cggtgcgccg gagaagaccg
    38461 gcggtcaggt cgtcgagctc ggcatcgggc tcgagattca cgcccgtgcc ggaatcgcag
    38521 atgtgggcca gaagggattc gttatcgaga gcgatggtga gaccgcggat gcattcgaac
    38581 agccggtcgg tcgggtcctc cgacccgctg gggtgcacat cgagcagctg cgccagcgac
    38641 aggtgcatcc ccgccaggcg ggtccatatg cgcccggctt cctcggccga ggtcggcccg
    38701 accgccatgt gggcggtcag ctcgtgccgg tcgcggtcga agaggaagag aaacgcccgg
    38761 ttgaaaccga gcccctgcga ggcggtggcc ccggtgagaa tgatgcggag aatctggcgc
    38821 agctccagcg aggacgacag ggccgcggcc acctcggcca ggatggagaa ctcgtcgtgg
    38881 gcgcggcggt tctggtcggc ggaggcctcg cggacgatgc ccacgatttc cgccgcctgg
    38941 tcgggcagcg gtacggcgca cagcgtcacc gccagcgggc gggcgccggg gccgctgatc
    39001 gcaatctctc ggcgcacgca ggcccggtcg ccgctgagga gacggtcgaa ctggtcgtac
    39061 acatcccccg gaaacaccgc cgtgaggttg gagacgtggc ggttctccca cggcgtcgtg
    39121 tgatcgatgc cgaggagctc agaaccggcc tcgttgcagt cggtgatgta cccctggcgg
    39181 ttgaccagga agaggccgat gtcgaggtgg tcgagggcca gcagcacggc tttgccgcgc
    39241 gccgggtcgc ggccctcggt cggggtctcc cccgccgggc ggggaaaggt gagcggccgc
    39301 ccccggcgca gcgcctcgcg gagaacctcg gcgtcgggcg gctcggtgac caccagcggc
    39361 gcggccgccg cgatcggctc caccgttcgt ttcctgatgt gtacttcctc ccccggctgc
    39421 tcccgctcga tgatatccga cgtggtcttc atgcttcact tacctcatcc ggatgacagt
    39481 tccgatccgc cgcacgaggc ggccggccag ctcttcctcg agacggcgga agaagacgga
    39541 cttgtccggc gcgctgatat ggaggtcggg atcgtgaatg tcattatcca cacttccctg
    39601 aaaaacttgc ggcccctttt tctgcacatg gaacggctcg gcgagggcca gccggccgcg
    39661 ggcgacgtcg atgacgcgca gttccccttc gatgaccccg agggcgacat accggtgggc
    39721 gaccagcggg atctgcatcg acttgcggtg ctccaggcgt tccgattcca cgcggacgat
    39781 gacgacatag gcggcgcccg cctcctgtcc ccaggcggcc agagccgcgg cgtcgccgtg
    39841 atccgggggg aaagccgggt gcgggggcgt ttccgcgcgc accgtcagaa tccgcaggtc
    39901 atcgtgccgg gtcaggcgct gcacgaggcg cgcctcgatc tcgcagtcgg cccagggcag
    39961 gtcgggggga gccacgacca gcgccacggt ccggccgggc gtttccccgg ggccggcgct
    40021 gacgagcaga agcccggcgg ccatggccgc ggacagtagg ccgagagcgc gccccctcat
    40081 gccgggctct tccgctgctc ggtgtagagg tagcgcgcca gcacccggcg cacgccttcg
    40141 ttgaagtccg cagcccgctc gccgaggagg tcgagctcgg cggccgacaa ccggtcacgc
    40201 aagtgctcgc gcgtaacgaa ctcgatcccg gtgagacaga cggcgccgtc gtggtcgcac
    40261 cgcttgacga ggccgaggac gttgtcgagg gattcgagcc cctcgaggcg gaagcgcatg
    40321 gcgacaatgt cgccttcgtt cagcggctgg gcgagttcga cgagcacgcc gccggcgccg
    40381 atattgagta tctcgccgct cagttccggt tcggcctcgg tcggcgtgaa attgccgaag
    40441 gcatccttga gacgccgcag ccacatcggg gagacgatct ccagccggac atagcggcgg
    40501 cggttttcct gttcaatccg aagcggccgg cgggcgccca cgcccgggtc ggttgcctcc
    40561 acgtcgtgca cgaatctcgt ccctgtgact ctcttcatac caagctcctg tttatactgc
    40621 gttctgttca tgcatccatg cgatgagttt gcgttcgatc tcccgctgca tgcgctggtc
    40681 gagggcgaag accgagccgg gcatcggccg cacggtgggg aacggcaggt gcccgttgcg
    40741 atcctcgcgg gcgatgaact gcaggccgac gtgccagcgg ccggtctcga ccgccgacga
    40801 gtggcgcacc tgtccgagca ggagcgacgg aaagcccagc tccgggccgc cgacgttgac
    40861 gaacaggtag gtgcggggtt cgaggacgct ggtgaaaacg atgagcgcgc cgccgcccga
    40921 gagattgacc gtctcggtct ccaaccagcg gaggcggctg aggccggtcg gggccaaacg
    40981 ggcaatcggc agcgccgcca gccggaacgg gcaggcgatc ggcacgcggc agaagcggcg
    41041 gcgccgcagc ggggtgaccg tgtcgccgag gtaaatgcgg cacccgcccc cctcggtccg
    41101 ccgcagaacc gcggacaccg agaccggctc ccccttgtac acgatctgca gaacgactct
    41161 ctggttgttg accaggttgg cgatcagtcc cgcgtggccg ctccggtcga tggtgatggc
    41221 cttgccggcc gccaccgtca cccggctggt gagcggtttg ccgggaaact gctcggcata
    41281 gagcgtgacc ttgcgcccca ccagggtgga gaggtcgaag cgctcgatct cgagtgcgtc
    41341 gagcgcctga tcgccggcgg tttcccgcac cgtcataaca gccctttctg gcgcagcttc
    41401 acctgctcct gaaagacata gttgacgagc ttgttctgca tgctccgcga aaattgccgc
    41461 gccgactccg gcagccggcc gagctcgtcg gcggtgaaga cctgcgcgag gtagtcggcg
    41521 cgcacaaact cgatgcccgc caggccgccg gcggcgggcc ggtcgacgcg gcggacgacg
    41581 gcggcgacgg gggtcgggat gccgatggcg gggaaaaact cgacatcggc gagcagccgc
    41641 gcaccgggcc ggagttcgaa gggagcctcg atgagcatgc cgccgccgga gaggttgagc
    41701 gacacggtcc ggtggacagc gtggggcggc agcgacgggc tgccgggctg gccgaggacg
    41761 gtcaccgtga gtgggcggcg caggtcgagg cggacgaact gccggcgctg cacccgctgg
    41821 atcgcctccg gggcgcgcag caggtaaatg cgtttctcgc gccgggcaat gaccgcaatc
    41881 tggctgtcga atcggtacac ggcgtcgtcg cgggtgaaga tcacgcggca gggagcgccg
    41941 ttgcggatca agtcggttcc gccgacaaac tcgggcgcgc cgatcacgag gatcccctgg
    42001 ccgaagtcct ccaggcgggt gatgtaccgc cccgcctcgg gaccgtcgcc gatcacgatc
    42061 tccaggcggt cccagaggtt tatttttccg gacgcggatg tcgtcgcagg cggtgtcacg
    42121 ggccggtcct ttcctcacgc cccctggggc gcttccatgc tcgactttcc atactcgatc
    42181 gccagttcaa ggattttgcg ggccagcgcg gtctgggtct ggtcgtgccg gttgcggtag
    42241 atgaactcct ggccgtggcg gatcgtgtgg ttgaagaact cgcgggcggt ggcgaaatcg
    42301 ccggtgcggc gggagagctc cgcgatcagg tacgaggcct ggatctgctg gttgcccggc
    42361 gcgatgctcc ggccgtcggt gaacgcctgc cggtagaaat ggaccgcctt ctgcagcgcc
    42421 tcgcgctcgc tggtcacgat cccatcccac gacttcttca gatcgtagag gaactcggcg
    42481 aaggtgggga agccggcgaa ggtcggctcg ctgttgatgt cgccgaggcc gacgccgacg
    42541 gtcttgaatt cctcgatcag cgtggtcagg tcggccaggc gctgctcgac gcccgtgatc
    42601 cgctcggtga gggccgcgcc ttcggcgagg aaccgctcgc ggtacgggag aatctgggct
    42661 ttcagctcgg aggtcagggt gtcggcctcg aactgcgact ggaccgactc gaggaactcg
    42721 gcgacccggt cgcgagccga cccgagggcg tcggtgatgc cgtcgtggcg cgtgtccagc
    42781 tcgtagagga aaccccgcat ccgcgagatc tgcgggtttt ccccgccctc gaggctccgg
    42841 aagacccagc cgatccgcag gtaaaagcgg ccgaggtcga ggcggctgta gtgttcgaag
    42901 agctgctcgt cgaaagccgc gaggtggagc ttgagaatgg ccgtctggtt ggggaaccgg
    42961 gccatgtcga tggccttgcc gaggcgcttg acaatcgagt cggcggtggc gagctggtcg
    43021 aggtgtttgt ccttggtggc tttgaggcgg taggtgcgga aggcgttgtc cttcttccac
    43081 tccttgtagg cgggcgtgta ctcgcgggag taatagcagt tgccgcaggt ggcgacaaag
    43141 aagaccagcg ggttgtagcc gtcgtacttg gggtagcgcc acttgatgtc gcgggggcag
    43201 aagtcgctgt cgcggccttc ctcgacgtac gcgcccatgc gcacggtttc aaattcgttg
    43261 atcgtgcggc agatggggca ttccaccttc gacaggaaaa acggggaatc gctggccatg
    43321 actcgctcct tgctcgtcgg ttaacttcgt tcgtagttga ggaccgccag ttcggcatcg
    43381 gagacgggca gcacggcctt gatctccgcg tcggtggcgc ccgtgcgcag catgccccgg
    43441 gcgagccgca acacttcggc ccggttgcgg cggagggatg cggcgggctc gccggccgcg
    43501 gcccgctcgc cgaaaccgac gtaccggacg tcgtcccgcg gccgatcggc cgcgagcggc
    43561 ggcgcaggcg cgggggcagc ggcgggcgac ggggcctcgg ccgctcccgg ccgggtcttc
    43621 cggcggaaca tcgaatacat caccatcgag agcgccccgg cggcgagata gccggtcgcc
    43681 gtgagcgcca ggtcgatcca catgtcattc gtcagcggca taacttccat cctaagtctt
    43741 caggcctgca ggtcgatgtg cagcggggcg ggcggctctt ccctgtcgtc ggcgtccgcc
    43801 gcgcggtcct gcgcctggtg tggttcggtc gggtcgtcgt cgcggttctc ctgatccggc
    43861 gggctctcct gtttgctcag gtcgacgtcg acgatgagcg ggtcgtccgg cccgtcgtcg
    43921 gcgcggcggt cgccgccggc cattctccgc ttgagcgccc gtttgaacgc ttcgcggtcc
    43981 tgctcctcgt tggccttggc cgtctcgttc acgcggtcgt gcggcggcgg cgggggcagt
    44041 ttgtcaatcg tttcaatcga gggcggcatc gcgcgcctgt ctccttttcc cgcgtgcggc
    44101 tcacccggtc agggcgaacc gttcgcggac catgctccgc agcttgctca ccgcgcgcgt
    44161 gtggatctgc gacacgcgcg actcggagat caccatgacc gcgccgatct ccttgagcgt
    44221 gagttcctcg tggtagtaaa gcgagatcac gagcctttcc tgctcggtga gccggtccat
    44281 ggcgacttcg aggaacgacc gcagttcgcc gcgctcgatt tcgccgagca cggtggcgcg
    44341 ggtggaatcc tgtatggttt cgatgcgcgg gacctggcgg ttgtcgtcct cgcggtagat
    44401 cacctcgtcg agcgagagga tctgggtgca ggagacgtcc tcgatcgcca ggtggagttc
    44461 gcgctcgctg attttcatgt gtttggccag ttcgttcttc tccggcgggc ggccgagttc
    44521 gttttcgagg tcgaccaggg cgcgttcgat ctcgcgcgac ttggcgcggg tggagcgcgg
    44581 cacccagtcc agcgcccgca gctcatcgag gatggcgccc cggatgcggg ggacggcgta
    44641 ggtttcgaac ttgacgccgc ggccggggtc gaaatttttg aaggcttcga tcaacccgat
    44701 tactccggtg ttgacgagat ccggcagctc caccgagcgg ggaaacccca tcgccatgcg
    44761 cgacgcgacg ttgcgcacga gggggatgta tttggcgagc aaccgctgcc gggcttccgg
    44821 atcccgcgat ttcgaatact gagcccagtc acgcgcggtc acatcccatt tccgagtctt
    44881 cttgggtgtg atctcgggcg tgatggtctc ggcgacgctc acgcggcgcg gtttgcagcc
    44941 gcgctttttc ggctcgactt tggcagtgct aaccatatgt ctgctatttc cttatatcgg
    45001 cctcagccgg gttaacctta tcgtccgcaa gggtgaaaag tccgcaccgg tccgcccctt
    45061 ccggggtgag ctcgcggacc agagaacggg ccattcgagt aagtgcctga acggcaagag
    45121 aatgttcggc ggccgctgcc agcggctgct gggccgccac cgccgtccgc accgccgcat
    45181 cctccggcac aaaacccagg caacgcagcc cctcgcccaa aaaccgctcc gtcaccgctc
    45241 caaacttctg gtgcaggtac tgggcctcct ccgcatccgc cgcccggttg atcaccagtc
    45301 ggcaggcgat gtcggcccgg gcctggcgga tgtgcttgaa cagaccgtag cagtccgaga
    45361 tcgaggtcag ctccggcacg accacgagga gatttatctc cgacccgtgg gcgatgaggg
    45421 tggcgatccg ggaccgcccg gagctgtgat cgcacacgat gacatcgtaa gcggcccctt
    45481 cggcgcgcag ccgcttgagc atggagccca ccgtcccggc cgaccaggcc tcgggatcac
    45541 acaggcgggg cgagacgccg agcaggtcca gccgctcggc gagcggggtg cggcactcgg
    45601 ccagcgacag acgccggtcg atgaattcgc cgataccgac ggcgcaggcg cggttggcca
    45661 ggatgtgcag gttgccgcca tagacgtcgc cgtcgacggc aaggacgcgg agaccgagag
    45721 ccgccagccg ttcggccagg ttgaaggcga ggatgctctt gcccacgccc cccttgccgg
    45781 agagcaccga caccaggcgc ggcccgccct gcgggacggc cgaggcgggg gcgacgaagt
    45841 gcagtctagt catcggccgc ctccctgccg gtgagcgtcg cggccagcgc ggccggatcg
    45901 gcggctttga gccgcccgag gccgccgggg ctgtcggaga agaagaccat cttgactttg
    45961 gcggcgcgcg cggcggcaat gaatacgccc tggcggcggg tcacatcgag catggtcgcc
    46021 gccaggtggg tgggcccgaa ccagcggtag cgggccgcca gatcgatcgc atcggcggag
    46081 cgcatcgtcg ccgggatcac caggaaccgg tagcgggcgc cgattttcgc cgccgcccgc
    46141 acgagcgcct cgcggcgatc gggctcggcc ggcagcgccg gagcatcgat cagcgtgatg
    46201 ctatctttgg tcccggcctt gtccgcgtgc tcgccggcga cttcggcgac atcgaggttg
    46261 agcaggtcgg cgtagccggc cagttcttca tgggcgccga ctttctgaaa atccacgccg
    46321 gccagcgcta ccttccgttt ctccacggca gcgaggtgcg cggccagttt gcccatgacc
    46381 gaggacttcc ccgccccggc cggaccgaca aagaggacgc ggtcgcccgg gcggaacttc
    46441 agatccggca ccatcagccg ggacagctcc ttcaccagct cgctgcggat tttcgccagc
    46501 gcgccggggc cgaggaaccg ttccggcctg gccagcagga aagcatcgat gaaatcttcc
    46561 ggcacatcgg cgtcgcggag ggaagcggcg accgcccgga tcgcggggtc ggcgatttcc
    46621 tcacccccgc gcgtcgcgct gaggcagacg aggcggtcga gggttgcctc gatgcgggcg
    46681 agccgatcct cggaggggac gggtgcttcg gcccccggtt cgtcggcaag cggctcgggc
    46741 cccgcgtctt cgaccgcggc cggggccggc tcgccgtcgg aagcgacggg gagccggttg
    46801 accgaagctg ggcggctgtc gaggatgtcg gacatccggc cgacagtcgg tttttccagg
    46861 caggcggtga tttccacctg gggccggttg taccggtcgg tgagctggcg cgtcttgagc
    46921 acgatggcgt cgccgcccat ttcccggcgc gcctgtttca tggcggccga ggcggattct
    46981 gcggtaaacg acttaatgat catcctgcaa cctcaccatt ccggtcgaga tcacctcgac
    47041 gtcggggaga atctcgttgt acgagaggac catgaggccc gggaagcgcg cctcggcgag
    47101 gcggcgcagg gccagtcgga tattgggcga acagagacag gccgggttga cgccggccgc
    47161 gtgctggagg tcgatctgct ccccgatccg gccgagcaat tgctcggcca gcgccgggtc
    47221 gatcgcgagc atcagtccct gtttggtatt ctgcacggag tcggcgagtg tctgctccac
    47281 cgccgggtcg atggtgaaga cgctgatccg cccgcgggcg tctttcgcca gttccgtcac
    47341 ctggcgccgg agcgccatgc gggcgtactc ggccagcacg tcggcgtcct tggtcgcgcc
    47401 gatgtagtcg gcgacggtct cgagaatggt tgccaggtcg cgcaccggga ttcgctcgcg
    47461 caggagctgc tgcaggactt tctgcatcgt gccgagcggg acgatctccg gcacggtgga
    47521 ctcgaccaag gccgggtagt cttccttgag cgtgtcgacg aggtgctgga cgtcctggcg
    47581 cgagagaatt tccggggcag cccggcggat cgtctcgatc aggtgcgtgg cgaccacggc
    47641 cgagggctcg actacggtgt actcgcgggc ctcggccacc tcgcggaggt tcgggagaat
    47701 ccaggtcacg gcgaggccga acgccggatc cttggtgtcg aaaccctcga gcttctcgcg
    47761 gacatagccg ggattgatgg caaggaggtg gtcggtcatg agttcaaagc cggcgacgcg
    47821 gagacccttg accttgatct ggtattcatt cggccggagc cggatgttgt cgcggatgcg
    47881 gatgggcggg acgacgatgc ccagctcggc ggcgagctgt ttgcggatcg agccgatgcg
    47941 gtcgaggagg tcgccgcgct ggttggggtc gacgagcggg atcaggccgt agccgatttc
    48001 cagcccgagg gtgtccacct tgagcaggtc ctcggtccgc tcctgggtgc ggtgttcctg
    48061 ggcgcgggag cgcttcttcg cctcctcctc ttccaccgcc cgcttcgcct gcagttgcgt
    48121 ggtgaggtag ccgagcccgc cgaccacgcc gccgagggcg aggaaggtga aggtcggcat
    48181 gccggggagg aatccgaaga tgagcaggac gacggcggcg accatgaccg cgcgcggctg
    48241 gcgggtgagc tggcgggtga ggtcggtgcc catgttcgag tcggcggcgg cgcgggtgac
    48301 gatgataccg gaggcggtcg aaaccagaag ggccgggatc tgcgtcacca aaccgtcgcc
    48361 gatcgacaag agcgagtagg tgcgcagggc ctcgttgata tccatgccgt tgatggcgat
    48421 gccgatgatg aagccgccga tgatgttgat cagggtgatg aggattccgg cgatggcgtc
    48481 gccgcggacg aatttcgaag cgccgtccat ggccccgtag aaatcagcct cgcgggcgat
    48541 atcccggcgg cgcgcccgcg cctcttcttc tgtgatgatc cccgcgttga ggtcggcgtc
    48601 gatggccatc tgcttgccgg gcatggcgtc gagggtgaac cgggcggcga cctcggagat
    48661 acgccccgca cccttcgtga tcaccacgaa ctggatgatg acgaggatga cgaagacgat
    48721 gaagccgacg acgtagtttc cctggacgac gaagttgccg aaagtctgga tcacgtcgcc
    48781 ggcgaaggcc tggccgagga tgagccgggt cgaggcgacg ttgagggcga gccgcatgag
    48841 ggtcacgatg agcagcatgc cgggaaagac cgacatgtcg agcggccgcg tgacatagag
    48901 ggtggtgagc agcacgacga gcgagaaggt gatgttgaag gcgagggcga aatcgagcag
    48961 cggcgtgggg atcgggatga cgagcacgcc gatgatgccg atcacgccga tcgccagggc
    49021 gatgtcggag cggctcacga gcaggtcgag caggccggta tggtctttcc ccgccttcat
    49081 ctcagtgcac tccctgtccc ttgaggcggt agacgtaggc gagcacctcg gccacggcgc
    49141 ggaagagcgc ggccgggatc atctggccga cgtcgcacat tttgaagagg gcctgggcga
    49201 gggggcgatc ctcgacgcag gggacgttat gggcgagggc gatttccttg atcttctgcg
    49261 ccaccagccg ctgccccttg gcaacgacgt gcggggcgcg gtgctcctcg ggcgcatact
    49321 tgagggcgac cgcgtagtgg gtcgggttgg tgataacgac gtcggcggtc ggcaccgcct
    49381 ccatcatgcg gctgcgggcg atctggagct ggatctgccg cacccgggcc ttgatctgcg
    49441 gcgacccctc ggtgtccttg aactcgtcct tgaggtcctg cttgctcatg cggatagact
    49501 tctcgaattc gtagcgctgg tagatgtagt cgaggacggc gatgacgagg atgaccgcac
    49561 cgaccttgag cgccagttcg agcgcaagct tgcccatggc ggcggcgatc tgcgccaccg
    49621 tcatatcggc gaagcggtag aattcgggga attcggcggc gatgacgcgg taggcgacga
    49681 acgcgaccac ggtgagtttc agcaggtcgc gcaggagcgt gaccgccgag cggaccgaga
    49741 agatgcgctt gaaacccttg accgggtcga gcttgtccaa ctgcggcgca gccgccttgg
    49801 gggtgatgcg gaaaccgacc tgggcgacat tcacgccgac cgcgatcacg gtcagcacgg
    49861 caaacaccgg gccgaggatg atgaagaact tgaggaggtg gttgcggaag acgccggtga
    49921 aggtcgggtc ggccagggcg agactgggcg cgttggccat ggtcgcccgc atgagcgcca
    49981 gcgcctggcc ggcgatgtac ggccccatca ggtagagggt gacgaagccg aggcagatca
    50041 cggcggccga gttcaactcc atcgacttcg cgacctgccc ctcctcgcgc gccttctccc
    50101 ggcggcgtga ggtcgccttc tcggttcttt cctggaaact ctcgtccgcc atttccctta
    50161 tgccttaccg aatgcgaggt agagcacgcg caattcccga tcgaggtgga gcgtcacttt
    50221 ctccagcacg tagccgaaga gcgggagcga cagggcaagc atggccagcc cggccgcgat
    50281 tttgatcggg atgccgacga aaaagacatt catcgtcggc atcgtcttgg cgatcacgcc
    50341 gagggcgacg tcggcgagga agagcgtgat catgaccggg gcggcgattt tgatcgcgat
    50401 gacgaacacg taggccgtgt acttgatcag cagttcgccg accgcccccg acgtcccggc
    50461 gacaccgggc gggatgacct cgtagctgtc ggcgaaggct ctcagcacga ggtggtggcc
    50521 gccgatcacg aagaagatca cggtcgcggt gaggacccag aacttgccga tcaccggcat
    50581 ctggctggag ccatcggtgt ccggaagctc gaccatcgca aatcccatct ggtagccgac
    50641 gagcgcgccg ccggtttcaa cgcccatgaa caggaggcgg aaaaccagcc cgatgatcag
    50701 cccgaccaga acctccttga ccgcgaaccc ggccagcacc cagagcgagt cgaaagccgg
    50761 gagctggggc tggcggatga cggaggcgat gacgccggcc aggaggatga tcaggccgat
    50821 ccgcaccagg accgggaccg acttgtcgcc gaacaccggc gccatcagga agaggccgga
    50881 ggcccgcatg atgacgagga ggaagacctg gagagcgttg gcgccgaagt ttacgaattc
    50941 aaacaagggt cacaatcctg gtttatcggt tatcgccttt tgctatgtca aatggtgtgc
    51001 cgaagtgaaa cggcgggggc ggtacaggta agtcgttggg agaaagcatc ttgcgggacg
    51061 ggaaaaatcg ggcgggggcc gccggagagc ggactgcgga aaggtttacc agcagttgtg
    51121 ggaaaaaagt tcagagcgcg gaaaggccgc ggccgggatg tcggcggcca cggaccgggg
    51181 agcagggcgg gcgatagggg ttacgacagg tcggtgcgcg ggtcggagga tggcgaagac
    51241 gcggcggcct ctccccgccc gagctgcgcg cgcaaccctt cggccagcgc ctccagggcg
    51301 gcggctttgt cccgcgtgcc gtagaacttg atgacccggc gtcccgactc gatgtgcatt
    51361 accgagagca cggggcgttt acggaattca atgcgcgccg tcgcggacaa cgcgatttcg
    51421 taggtacgta ctttgaacca ccgccgcgtg atgaaggcca gccgcgcggg gccggagacg
    51481 agcagcccgg cgtagaaccg cccgcccgaa aatcccttgc ccgaatatac ccaggcccgc
    51541 cgccgcggcg gatcggtcgg cggaagggcg ttgagggccc ggcggaaggg gtcggggagg
    51601 tcggtgtcag aaaggaccgg ggccccctca tccccgcgca gcgatccggt caccgcggcg
    51661 aactccattt tcgcaagacg gaggagtttc ggcccgagcc agaacatcag cgccagaaac
    51721 agcccgaccg ccgccatccc cagagcggga tagcgcagga agaggaggct gccgacgacg
    51781 acgaggacat cctcaaacag actcaccagg ctgttggaga ccggttccgg actctgattg
    51841 acgatgagcc gggtcgccgc tttcgtcgag tgggacgaca gactgaggcc gccgcaaaca
    51901 agcgcggcgg cggtctccca caccgggtcc atcccctgga aggcggccag accgagcgcg
    51961 gccgcgccaa gcggtcgaac aaacgtatgc acggcatccc acgccgaatc cacccagggg
    52021 atcttgtcgg cgatgaattc gaccgcgtac aggagaccgg cgatcacgag cacggccggg
    52081 tgggcaagca cctccagacc ctgcaggggg agggggagcg aaatgatgtt gtagcggacg
    52141 gcaagtccga ccgtcagcgc ggcggcgtac aggttgagac ccgacagcag cgccagaccg
    52201 aacatcgacc ccaaaagcga gagtgtctgc atgcggcagg tttccttctg cacgcaagct
    52261 accgctctgc cgaggaactg tcaacagcgt tcggccaacg ggacaacggc ccacaggcaa
    52321 gtcaccatgg agatgcccca gagcggacat ttcgcaggcg gttacgaaac atgcccgcgg
    52381 aggtctccgt atgggagact gggattgatc ctgccgatgg ggattcggcg ggcggccgcc
    52441 ctcgtacaga cgcgcgaggc gtgcggagcg gagtcacatc atccgcacgc aaacgaggcg
    52501 gcgccgcgtg cgccgcacca ggtcggaggt cggcatgtcc atctcgcaca gagctctggt
    52561 cgccggtggt cggaaggcac gaccatcccg gctgcgggcg gttgtctgcg ccgccctcac
    52621 tctcgtcctg ctcgccgcgc cgggcagatc cgcagccggg gctcccggtc agctcgggac
    52681 cgggcgcgcc ctgattccca tcacgcccga aaacgccccg aatgtcgcgt tgctggcaac
    52741 gctgtcggaa cacacgggcg gcgtgtggac ggcggcgttc agtcccgacg gccgtcaact
    52801 ggcctcctgc ggcgaggacg gccgggtgat catccgcgtg gttgattcac tgtcgcacac
    52861 ccggacactg ggcgattccg agtactgggt ggtcgggctg gcgttcagtc ccgacggttc
    52921 gcagttggcc gccggggagg ccgacgggtt cggaggagct tacggcccga tccgcatttg
    52981 ggacgcggcg gccgataccc tcgtgcggac gctgtcgggg cattacgggg gcgtctggag
    53041 tctcgactac caggagagca cggggatgct ggcctcgggc agcttcgatg ggacggtgcg
    53101 gatctgggac ccgcagaccg gcgcgccggt gcgcatcctg agcggacaca cggcggcggt
    53161 gctcgcggta gatttcagcc ccacccagga cctgctcgct tcgtcgggag tggatttcac
    53221 ggtacgcctg tggcaacccg cgaccggcga caccgtgcgc gtgctccgcg ggcacacggg
    53281 caatatcggg tacgtggagt tcagtccgga cggattggcg ctggcctcat cggccgacga
    53341 ccggacggtc cggttgtggg atttttcgga cggcagtctg ctctggtcgc gcgcggccgg
    53401 ccagggctgg gtgaactgcg tgactttcag cggcgacggg cgcctaatgg ccacctgcgg
    53461 ccacgacggc tcggtggtgc tgcgcgacgc ctccaccggg gccgagctca aacggctggc
    53521 gggacaccag ggtccggtgc tgcgcggggc gttcagccct gacggcacgc tgctggccac
    53581 cgcctcgtgg gacctcacgg ttcggctctg gggcgtggag acggacttag acggcgacgg
    53641 agcggacaat gacagcgaca actgcccgaa cgtccccaat ccggggcagg aggatgccga
    53701 cggcgacggg atcggcgatg cctgctgctg cactgtgcgg ggcgactgca ccggcgatgc
    53761 ggcggtggga gtcgcggatc tcacgtactt catcagatac ctcttccgcg gggggccggc
    53821 ggcgggttgc ccgtcgcacg cggacatcaa cgcggacgaa acggtgggac tggcggacct
    53881 gacctgcctg atcggctttt tgttccgcga agggccggct cccgcggcct gcccttcggc
    53941 ctggcggtga ggggagggtc gtgtgccgcc ggagggcgcc gacggccgga cagctctacc
    54001 ccacgagctg cgggatgagg tcgaacatgt gtcgcgtgaa gtcggtcgcg atgttgatca
    54061 tccaggggag aaagatcagc agcgcgagcg cgaccgcgag gatcttgggc acgaaggtca
    54121 gcgtcatctc gtgaatctgc gtgaccgcct ggaagatcga gatcacgagc ccgacgatca
    54181 ggccgaacag gagcatcggg ccggagatca agagggtcac cgaaagggcc tcgcggccga
    54241 tggccaccac catttcctgg gtcatatgat cagtttctcc gtcaatggac cggtcgtcat
    54301 cacgccgggt tcatgagggt cccggccacc ttcgttacat ccggaacgac tccacgagcg
    54361 atttcaccag caagtaccag ccgtccacga gcacaaacag gaggatcttg aacggcagcg
    54421 atacgatcac cggcggcagc atcatcatcc ccatcgacat cagcaccgac gcgaccacca
    54481 tgtcgatcac gagaaacgga atgaagagga cgaaggcgat ctggaaggcg gtgcgcagtt
    54541 cggagatcac gaagcccggc acgaggacgt ggagggggat atcctccggc gtctcaggcg
    54601 gctcgagctt ggccatgtcg acaaagagcg cgagatcctt ctcgcgcgtc tgcgccagca
    54661 tgaactggcg gaagggtccg gtggctttgt cgaaggcctc cttcccgctg atctgtttgt
    54721 ccaagtaggg cttgatcccc tcgccgtacg atttctccac ggtcggagcc atcacgaaga
    54781 aggtcaggat gagggacaag ccgaccagca gttggttggg cggcatctgg tttatcccca
    54841 tcgcgctgcg caagaacgac atcaccacga cgaagcggat gaaggaggtg accatgatga
    54901 ggatcgacgg ggccagggcg aggatagtca gcaaaaggac gatctgcagg gtggtcgaca
    54961 gctcctgggg attgtcggcc gacccgacct ggagcgagac tttcggcagg ctctgggccg
    55021 agacatcggc cgcggcgagc agagaggcgg ccgcgacgag ggcgccgaga acaagcagct
    55081 ttttcatatc cgatatttct tgtcgcgcga catcctgtcg cgcggtggcg cccgcaggcg
    55141 cggtaacggg cacttaggca gtcaggactg tgtctttcct tttcaagcgg agcgtgcgca
    55201 gtttcccgga cgccgctttg agcacgttcg gaaacgtctc cgcctctccc ccggccgccg
    55261 gggcggcgag gatctcggcg gtcttctccg gagacagctc ggtcagcagc gtgatcgcgg
    55321 tttcggtgac cccgaccagg acggccttgt cggcgacccg caccagcgag atcgtcttgc
    55381 ggggggccac ggcggcggtt tcgatgatct cgagccgccc ggagcggccg aacccgccgc
    55441 gcttgccggc gagccgccgg agggcgtaaa tgccgccgta gatgcaggcg atgacgatgg
    55501 cgagggcgct cgcgatacgc accagcgaag ggagggccga cccggcgaag ggtgcggcgt
    55561 cggtctcgga gttcggcgcc gcctgcaggc gtccgaggtc gacgccgcgc tcggccgagg
    55621 ccgggccgcc gttgattagc gcgaggccgg cgatggccgc gccgaggatg agggcgaggc
    55681 cgatggagcg ccgccgggtc agtttactgt tcatcgctca gcagtttcag ccgctgctcc
    55741 ggcgtcatga gttggacgat gcggacgccg aagttttcgt cgatgacgac cacttcgccg
    55801 cgcgccacga tttttccgtt gaccagcacg tcgaccggct cgccggccag cttgttcagt
    55861 tcgacgaccg atccgggccc aagcgagagg atgtcggcga tcgacatccg ggtgcggccg
    55921 agctcgatcg agagcggcag gttgacatcc atgagcaggc cgatgttgcg gggggccggt
    55981 ttggccgccg gttcggagag gtgctggaac tcggcctcgg ccacctcggc cgacccgaac
    56041 cggatgtcct cgggcgcggt cttcgattgc tcctgcggga gatcctccag catcgccatc
    56101 atggccgcgt cggcctcttt gccggacagt tgttcccggg cggcggcctg ccggaccgct
    56161 tcgcgggcgg ccgtctcggc ctcagcgccg ggctgggccg cggcggccgc gccgccgacc
    56221 tcgtcctgcg gctgcgcgtg cgtctgatcc gtcattgctt cttctcctcc gatgagcgtt
    56281 cacatacatc gataacctgg aacgcgcgct tcttgcccgc caggccgggc cgcgccgtgt
    56341 acttgcgccg gcggttgacg aagacctcga tgggcgcgtt gattttcttc tccgacggca
    56401 cgacatcccc gatgcgcagg tcgaggaact cccgcatgcg gatggacgtg tcgagcagga
    56461 tggccgacag gtcgacggcc acgtcgttga gattggtgag attcacgtcg cggtcggatt
    56521 tctggagccg cttcttggtc gcatcgatcc agttctgggc cgagagcttg gtgatgatcg
    56581 gctcgagcgc aacgtagggg tagcagatgg tgagcagccc ggtcgactgg aagatcttca
    56641 cctggaacga gaccaccacg accgtttcgc cgggcgggac gatctggatg aactgcgggt
    56701 tggtctcgaa cgatttctgc tcgaccttga tctcgacgat gttctcccag gaccgttcga
    56761 ggtcgcggta gagccggtgg gtgagccgtc ccatgacgga gcgctcgatg ggggtcagct
    56821 cgcgctcggt ctcgaggatc ttgccgctcc cgccgaacat gcggtcgatg aacgagaagg
    56881 tcagggtcgg gttgaagtcg accaggctca gcccgtcgag cggctccgcc gcaaacgtgc
    56941 agctgcacga gggcgtcacc agcgacatga tgaattcagt gtaggtgatc tggtcgacgc
    57001 tgacgagatc gacgtcgacg atggtccgca tgatcgtcga cagcgacgag ctgtagtgcc
    57061 cggcgaagtt gtcgtgcatg ttctccagcg tgcggagctg gtctttcgac acccggttgg
    57121 ggtgcttgaa atcgtaggcg aggacggacc gcatgcgatc gtcgcgcccg cgctccgcgc
    57181 ccggggccga ttcgcccgac gacaccgtcg tcagcagggc atcgatttca tcctgggtca
    57241 ggatcttcgc cacgtcggca ctccctcact gcagcacaaa gtcggtgtag tagacgccgg
    57301 ccagctcgtc cgtcttcatg agctccccca cccgcttctt gatctggtag cgggtgatct
    57361 ccttctgccg ggtgtcggtg agctgggcga ccgttttcga ggacaggatc gtgatgaggg
    57421 cgtcgcggat agcgggctcg cgggcgcgga aggcgatcat cgcggcctcg gtcttcactt
    57481 cgaacccgat cgacactgag aggaagcgcg agccgccggt gccggccggg ttgatcacga
    57541 ggtcgtcgat agtgaagatg cgggcggcgt cggcgccgcc gccgtgcccg cccccggact
    57601 ctttcctggt ttccgccggc gcggtctcgg cgtgcggcgc gggggcggcg gattcggcgg
    57661 cccccgccgg cgtctccggc gacggcgcga gcatcggttt gaccacgccc agagccagca
    57721 cgatgccgac gacgacggcg gccaggccga tgccgccgaa caggagcagc ttcttcttcc
    57781 cgcccggggc cggggccggc tggtccgggg gaggcgcggc cgccgcgggg gttacggccg
    57841 tcttctcgtt cttgtcactt tcgttcggca tggcgttctc ctatcgcgcg ggcccgccgt
    57901 ccggcgccgg gggcgccggg acggcggtcg cgctggtccg gcgcgccccg acccggttcg
    57961 tccgaaagac gaaccatgat ctcgccgtcg acattgacga acgtcaccgg tcccggaagc
    58021 gattccagga agcggatgag ttcggcggtg tactcgaacg gcacttcgtt atgtcgggtc
    58081 acgcgaatca tctagtcgct acctcttgat gttgaccagt tcgtcgagca tgttgtcgga
    58141 ggtcgtgatc acccgggcgt tggcctggaa gccgcgctgg gcggtgatca tcccggtaaa
    58201 ctcctgggca atgtcgacgg ccgagctctc cagggcgccg gagaagatct gtccggagat
    58261 ggtctgcccg gcgacgccct cgacggcgct gcccgagttg gcgctctcct ggtacatcga
    58321 cttccccgct ttccgcagac cggactggtt gaagaagtcg gagagcatga cctgggcgag
    58381 caggcgggtg acgccgttgt tgaagatgcc ggagatgttg ccggccttgt cgatcgcgat
    58441 cttctcgagg atgcccacgc cgtagccgtc ctggcggacg atcgaggcgg tgtgggttcc
    58501 ggaagcgaac ccggtgaggc cgtcgaaact gaacgccgtg cccgggttga ccacgacttc
    58561 catgttttcg gcgccgttgt tggggttgat cgtgatcgct tcgttgccgc cgttgtaggc
    58621 gaacgagttg agcgagccgt cggggttgaa ctggacatac cccgacccgc ccgtgagcac
    58681 cgtctccgtg ccgagggtgg cgacagacca ctcccagcgg ttggtgaggg cggagcggaa
    58741 gaactcgatg gagagagtgt ggcggccgcc ctgggagtcg aagaccgtga tcgaggcggt
    58801 gtgggtggtc gactcggtgt cgataaccag gtcgttgccg ttggtggcgc cgatgccggc
    58861 ctgtccggcg gtgatttcga cgccgccgtc gcccagcccg ctgagcccgg tcaccaggcc
    58921 ggtccgctca tcgacaacca ggccgagctg ggtgacaaac ggtccgccgg ctgtcccgga
    58981 tccctggagg ggggtgaagg tgtcggtcgc cacgagcgtg gtgtcggcgc cgccggaggt
    59041 ggccacggtc gcgcccaacc cggcgccgaa gatcgtgttg agcacgtcgc cggccccggc
    59101 cggggagctc acgatgttgt aagtggcgtg gtcgccggcc cgcgtccgca tgatcgtgat
    59161 cgcgccggat ccgtcgaggc tcgccgacac accggcgatc tggttcaggg aggcgatgag
    59221 ttcctcgacc gtggtggtag cgctcagtcc ggagatcgtc tccgtccggg cgccgtcaat
    59281 ggtcaggctg aagtcggaga agtcggtgac gccgagggcg ccgagggtct gcgcgccggt
    59341 cattccggcg gtcgcgctgg tgaccgtgtc ggcaatcgcc tggtcgccgg tgatgctgac
    59401 gcggtgggtg ccgcccacgc cgtcaagcgc ggtcccggac accaggagga cattggagtc
    59461 gcccgattcg acgagcgcgg cattggcgtc gctcgccgag gcgtcaagat tggccgagag
    59521 ccagatttcc tcggtggcgt tggccggatc ctgctggccg aaggggagcc ggatgggtcc
    59581 ggtggtcgcc agcgagggga tctggccggt gttgtcggcc atgcggccga ggacgtacag
    59641 gccgttggag ggatcgacga gggtggcctg ggagtccagc ccgaaggcgc cggcccgggt
    59701 gtagaagcgg ttgccgttgg cgtcgccgag gatgaaaaac cccgagccct ggatggccag
    59761 gtcgaggatc tggccggtcg tctcgagccc gccctgctgg aagaggttgt cgatcgaggc
    59821 cacttccatc cccagcccga gctggaccgg gttggtgccg ccggtgatgg ccgaggggcg
    59881 ccccgcgccc ttgaacgtct gcacgagcgc ttcctggaaa ttcacacggc tggttttgaa
    59941 ccccacggtg ttgatgttgg cgatgttgtt gccgatcacg ttcattttca cctggtggtt
    60001 cttcagtccg gacacaccgg catacagtga tgccatcatg atgtgcgtac ctccgttttg
    60061 ccgtcgccgg gcctctccat cccaatggag gccgacccgc ggtgcgatgc agtgttatgg
    60121 ttggtgttca tcacagttct cacaggatga cggccgagtc gatcgacgtc accacgccct
    60181 cgcgcaggtt gtcgcggtcg aaggcggtga tgacggtccg gttgggcacg gccacgacca
    60241 gggccgcctc gtcggtgagg acgagcgttt cgcgcgagcc cttggcggcg gcgcggtcga
    60301 tcgcgtcggc gattttcgcc agcgtggcat cgctcagcgc gatcccgcgc gagaacagcc
    60361 gctgactggc gtgtttggag aaatgcacgg cccggtcggc cgccagttcg cgggagaaca
    60421 tctcccggaa cgacccctct ccggcctggg ccggcccggc gggggcggtc cggttggcga
    60481 tctcgatcag gttgaccggg cgctggccga ctgctatcgg ctggggcgtg atcttcatcg
    60541 taacgtcctt ccgcttctcc gtctcccggc gtcactcgcc gccgagttgc ccctcttcgg
    60601 caatggccgc cacgtcgccg aacgggatct ccatgccgtc gacgcggaaa taggcggcgc
    60661 cgtcgcggta ggtcaccgct tcgaccacgc cgcgcatgcc cagcttggga gtgtattcgg
    60721 cccccgcgcc gttgaccgcc cgcaccgcca ccgcgtacga tccctccggc acccggttgc
    60781 cctgggcgtc ggtgccgtcc caggtgaaag tgtgtttgcc ggaggcaagg ttctcgtcgg
    60841 tgagggtggc gaccacctcg ccgtcggcgt tggtgatctt gatttccacg ctggccacga
    60901 agcgatcggt ggtgatggtg agcgacggct cttttgaacc gtcgaagtag atcgtgtcgt
    60961 aggaggcctc gacctctttg ccgatgagtc ccgcggccat gacgttgttg atggactgca
    61021 tctggaggta gtcccacttg ttggattcgg cgatgccctc ggcgatgttg gtcatctgtt
    61081 cgagcgagga gaactgggcg agctgggcga tgaagtcttc atcctccatc ggattgagcg
    61141 gatcctgatt ctgcagtttg gtgaccagca gctggaggaa gtcgtccttg ccgagctgct
    61201 gcaggcttcc ggtctgcccg ctgttggcgg ccgccgacat gagtccgccg acggtggtgt
    61261 tggtgatttc gctcatggtc gtctcctatg ccaaaacatc caccccgccg gcgccgagat
    61321 agccggacga tccggtcgcg gggaccgcgg gcggggcggg ggcggtttca tcgtctcggt
    61381 cgaagcggcc tccggaagcg ccggagcgtg gccagtgcgg cgcgtgctgc gcaaaccact
    61441 gcccgcgcgc cccctgctgg ccgatcgcca cttcgatgtg atccactttt atgtccgccc
    61501 gggcgagggt ctcgacaagg cggtccaggg aattctcaat cgtgtgtttc gcgcgggcgg
    61561 tctcgacgat gacccgggcg gagagatgct cgtcgcgcac cgtcaggtgg agccgggccg
    61621 ggccgagatg ctcggggtcg atgcgcagtt cgaccgagtc gccgccgggg cggagggccg
    61681 tcttcacgtc cgccggcagc gtgaaacggg cgggcggcgt ctgcacccgc tcgccggcgg
    61741 ccgccgtgcg gagatcgtct gcgccggcgc ctttggccga cccggccagg gtcgcagccg
    61801 cctcgccgga tgcatcgggg gccttgcctc tgccccctgc cagcgtgagg aaccggatct
    61861 cgcgggtcag agggaacaga cggctgtcgg tgaagtgtcc atcggcagtg tctggtgcgg
    61921 cggcggccgg gcgcggcgtt gtcttggcgc ccgccagggc cgcccccggg gttccgggcg
    61981 aagccgggcg cgccgcccga ttctgcagga ggcgaaaacg aatatcgctg ccggcgggcg
    62041 tggttacaat caccacgtcc cgaccggcat tttcagcgag gatttcaaat ttgcagggag
    62101 cctcggacga agcagccgcg ccgcccgcct gcgccgatcc gtggccggcg gcaggcgtga
    62161 ccaccagctc cttgaggctg accttctcaa accagctctc gagtgcggtc ggcgatgcgc
    62221 cgctctccag cgggacccgg gccgcggacg cagggacgga cacgtgggga gccgggatct
    62281 ccgacggctg cggcaacggc agcgaaatgc gaatggccgg gtgccccggg tctttcgggg
    62341 aaagatcgag catcagccgg ccctcggccg cgcgcgcgtc ggtgattatg tacgcaccgg
    62401 ccgggagcgt gtcgagcggc tgcggggtaa gatcctgaat cagttgcagt gcggccgggg
    62461 tgggaacgaa agttccggtt ccgatagatc ccggcgggtt cgtcgccgcc ggggagtatt
    62521 cgtccgagac agcagcggcc tggatcgccg cccgcccctg gaggaccgac cgggccttgt
    62581 cataaatgtc accgctcgtc gtctccagcg cctcgaaatc ccatcccggc gaaacgagga
    62641 ggtcgtccaa gagtgcgcgt tcaccgtcgc gtgtcgccgg gacaagcgga cggcaggcgg
    62701 cggccagagt atcgtcggcg ggccgagcgg tctgaccggg gaccgaagcg gcctgcgtcc
    62761 ccgatggcat cgaggcggtt ttcgccaacg gaaggagcga tgtcacccga gacgggccga
    62821 gggcggtctg catgagagcg ccgaacagca gttccggcgg agcgcccgcc tctttcccgg
    62881 ccgtcgcccc ggagggttcg actgtcggca gtggcgcgac tccgagcagg aggttcataa
    62941 tatcgggtga cagggtcggc atcgttattt ttccgcaatc gtgatcatct gtttggacag
    63001 gcgcgccgcc cgctcggggg ggagcagttg gagcacctcg gaggcgttct tggctttcat
    63061 gcgcggcagg atcgacacga ccgtctcatc gtcgaggttg gtcatgagct tggccacggc
    63121 gtgcgagtcc atcccgtcgt agagcttggc gagaccggcg gtgcgggccg attcggcctg
    63181 ttcaagcacg agcatctggc ggctgacctc gttctcgcgg cggacgagat cgttctccct
    63241 ggtcttcagt tgcgactccc attgggcgag ccgtttctgc tcctcggcca ggtggtcggc
    63301 cgtcccgggt gcgccctcga tcgccggtgc cgtatcaccg gcggccgcac tctccgccgg
    63361 gtcgtagtcg agcacgttca ggttctcctg aatgagcgcg agcatgtcgg agtcgtcctc
    63421 gccggccagg agactgtcga ggatctggtc gtcggtcagt tgcgccgcct gggcggcgcg
    63481 gttcccggcg agagtgctgt cggtgtcgcc cggtgcgacc ggcgcggtgg acttcccgcc
    63541 gagcagcagg agcgttccga ccgacgccgc cgcgatcacg gcgaggccga gaccgccgta
    63601 gaggaggtag gtcagaagtc cctttttggc cgggggcgga ttcggcgtgg gcgcgggggt
    63661 cgcagccggt gcgggcgggt tgttggacgg gttttgatcg gtcacactaa tactccgagc
    63721 tacatttttc cgagggagca ctttgcacag cccgtgccaa ttctcccgcg cccggtgtaa
    63781 gtgtcgcatt gattggaggt tgcgggctgc tcgcggcggg cgcgggccgg ccggaaagcg
    63841 gcccggcggg aagaattttc cgcttcgatg ggaaagatgg gatcgccgcg gggcgcggcg
    63901 cgggaccgcc caaaatctgt atttatcgac tgcggaacac gatttttctt gacttatccg
    63961 cacctgaccg gtacaatggt cgtgaatgaa acgaaggatt cccggcactg ctcttatcga
    64021 accttggcga cacactccat gacatctcga aaggcggacc gtagggaccg atgcgcaata
    64081 gtgcgcttga acgtgctgtg ttctgcaatg tgcgaaaaag aatctccgca gggcagggcc
    64141 ggccagacgg agagttggta gaatgaagat ctacattggg aacatgtcgt actcgacgac
    64201 cgaggatcag ctgcgccagg cgtttgaagc ctttggcgag gtctcggagg tcaacctggt
    64261 catggatcgc gacagcggtc ggccgaaggg attcgctttc gtcgagatgc cgaatcagga
    64321 cgaaggcaag gcggcgatcg cggccatgaa cggcaaggag ttcgacggcc gtacgctgaa
    64381 cgtcgacgag gcccggccgc gccgtgaagg cgcgggcggc ggcggcggcg gacgcggcgg
    64441 acggggcggt ttcggcggcg gcggcggcgg caatcgtcgc ggcggctggt gagcccccga
    64501 gttcccaagc cctgacatga gaagaaggcc ggtgtgaacc ggccttctct tttttttgcc
    64561 ttgggcggcg gggccacgcc ggccggccgc tcagttcacg gcctcggcca gctggaggcg
    64621 gcggtagatc cgcgcgtagt cgccctcggc gtcgatgagg gcgctgtggg cgccctgttc
    64681 gacgacgcga ccgctgtcga gcacaaagat ccggtcggcg cgctcgaggg tgtcggggcg
    64741 gtgggtgatc agtacggccg tcatctcggg catgacctcg tggaggcgtt cccacagctc
    64801 ggcctcggtg cgggagtcga gcgcgctcgt gcagtcgtcg agcacgagaa ttctcggttt
    64861 gccgacgagc gcgcgggcga gagccaggcg ctgcttctgc ccgcccgaga tggccactcc
    64921 ccgggtgccg atcggggtct ccaacccctg cgggaagccc gcgatttcgc cgcggagccg
    64981 ggcgacgtcg atcgcccact cgagcagcgt ctccgagatc ccctcgcggc cgaagatgat
    65041 gttgttgcgg atcgtgtccg agaagaggac cggttcctgg ggcacgaacc cgatcgcgcg
    65101 gcggagctcc tcgaggcggt actcacgcag gtcgaggccg tcgagccgca cggtccccgc
    65161 ggtcgggtcg accagccggg ggatcaagtt gaccagccag ctcttgccgg agccgacttt
    65221 gccgacaatc gcggcggtct ggcccggcgc gatctcgaac gacacgtcct cgaggatctt
    65281 ccgctccatg ccctcgaagg cgaagctgac gttctcgaat gtcagccgcc cctggccctc
    65341 gccgttgggg ctgagccggc cgatgtcggc caccatcggc ggcaccttct ccagctcgac
    65401 gaggcggtcg atcgagaccg ccgactgccg cgacttgacg aggaactgcc cgatgtcgaa
    65461 catcgggaag accagccagg tggcgtagta gatgaaggtg gcgatgctgc cgatcgacag
    65521 gtcggcgtag agcacgcggt agccgccggc gagcagcacg atcacgaccg cgaactgcca
    65581 caggtagtgg tagagcgagt ccatgacggc cgcgatccgc acggcgtcga tctcggcctt
    65641 gcggcgctcc tcggcggcgc ccgtgaattt caccttctgg gcctgctcgc ggacgtacgc
    65701 cttgaccacc cggatccccg agaagcaggc ctccatgacg tcgttgaagt cggagatgcg
    65761 cttctggagg gcatcgtagc gccggtccag cagcgagctg ctgcggaaga acaggacgac
    65821 gatgaacggc agcggcaggg cggtccaggc ggtcaggccg ggactgatcg tgagcatcga
    65881 ggccacgatg aaaaccaccg cgagcgtcgc ctcgtacagc cggaagatcc ccgagcaggc
    65941 gaaccaggag agcttgtcct ggacgtcgtc ggtcatgcgc gtgagcagat cgccggaccg
    66001 gtagtggttg aagaagtccg ggcccttggc cgtgatgccg tcgaaagcct cctggcggaa
    66061 gtgccactcg aggcgggtgt tcatccaagc ccgccaggac tggaagtagg cgtagagggc
    66121 gtaggcgatc aggccgaaca ggatgaacgt cacggcgtag gcggcggtcg tgttcagacc
    66181 gatcgcgcgg ccgagttcgc tcagccaccc ggccgcgccg gtccgggggg cagcgcccgt
    66241 gttgaggtag tcgatagtca gcccgatgag gcgcgggagc gccaccatga gagccgtctg
    66301 cagcggagtg agcacgagca ggatgacgag cactttgggg tagtgccgcc agtaccgcca
    66361 gagccaccgt attttatccc gcatgggtca ttactccgtt tcgatgcttg aactgcaggt
    66421 ggaacagctt cgagtagtag ccgttcgcca ggatcagttc ggtgtgcgtg ccgcgctcga
    66481 tgatttcgcc gcggcggatg acaaggatct ggtccgcatc gagaatcgtc gagagacggt
    66541 gcgcgatgat gagcgaggtc cggccggcgg tcagcctgcg cagcgaggcc tggatggtcc
    66601 gctcggtttc cgggtcgacc gagctggtcg cctcgtcgag gatcagcacc tcggggtcga
    66661 cggcaagcgc ccgggcgaac gagagcaact ggcgctcgcc gcggctgaag ttggagcctt
    66721 tctccgacac ctcggtcttg tagccggcgg ggagccgccc gataaaggca tcggcctcga
    66781 ccgtccgcga ggcctcgaca atctgcgcct cgctcaccgc accgttctcc aggccgatgt
    66841 tgccggccac gtcgccgggg aacaggatga tgtcctggag gaccagcgcg aaccgccggc
    66901 gcaggtcggc ctggcgcagg tcgcggatgt caacgccgtc gaccgtgatc cgtccgcgct
    66961 gcgggtcgta cagccgcagc agcaggttga tgacggtcga tttaccgccg cccgtgaccc
    67021 cggcgagggc gacccgcttg ccggcgggga tgtcgaacga cacgtcgcgc agcacccagt
    67081 tgtcgtcgtc cccgtacgag aaccagacgt tttcgaagcg gatggtccga tggagaccgg
    67141 gccagtcgac gggccgggcc gggtcgagca ctttcggccg gatcgagagg agggcgaaga
    67201 tccggcgggc gccggcgatt cctttctgga agacgccgag ctgctcggcg gtccgggcga
    67261 tgggctcgaa ttcgcgccag atcagcagga tgaacatgac catggtccca gaggagacgc
    67321 cccacatcga cccgagcaac aggaccagcc cgacttttac gaattcgatg tagccgagca
    67381 ggttgaagaa gatgcagacg ccgatgttga cgtaggagtc ctcgcggaac ttgcgctcac
    67441 tggcgtgcga cacgcgcgcc cgcgcatagg cgccgcggtg gaagatctgg acgatcgaca
    67501 tgccgtgcag gaactccgcc agcgccgcgg tgacctcggc cgcccgcttg cgcacctcga
    67561 ggaagcggtg cgaggtgagc cggtggaaaa cgtaaatcat gagggccagc gcgggcagcg
    67621 acagcgacag cacgagcgcc agccgggggt ggtggtagaa catcacgccg aggatcccga
    67681 caatcaggac gacgtcgccc acgatgagca cgaccgtgtt ggtgaagagc atgcgcagcg
    67741 actcggtgtc cgattccacc cgcgccatca cgcgcccgac cgggttcttg tcgaagtagg
    67801 agacgtcgag cgagaggatg tggtcgaaca gcttgcgctt caggtcgagc atgacattct
    67861 gcccgatcgt ctcgagcttg acgcgctgga tgtacatgag cgccagggag gcgatctgga
    67921 gcagcaccac ggtcagcccg actttcacga gcggggagaa atcccgctcg gcggcggggc
    67981 gatccagttg tccgcgcagg atcttgtcga tcgcctgctg gatcagccag ggccaggcga
    68041 gggtcatggc ggtcacggcg accaacagga gcaggcagag cgccagacct tttttatgag
    68101 cggccaggaa cggcagcagg ccgcgaaaag cccggcggac gctgacgtcc ttttcgtctt
    68161 tgccgatgtc ctcgtgttcg tacaattccg cagtcattgt cggcctcgtt cacagtctcg
    68221 attggtgcgg cggagccgcg gccgatccgg cgaaaaaaaa cccgccggac cctgtctcgg
    68281 tgcggcgggg gaaaattctc tcgtgggagc ggcctaaaca gacacactcc tcccgccgca
    68341 tttcttcccg ttcggcatgg cagccgagat gtagacctta agctggatca cggaatcctc
    68401 ctgagtctat cactatgcag cggtcaacaa ttcatcacag cattaggacg gcgtctccgt
    68461 cccctttgtt tcgccaagta acgccttttt ttgtttttgt caatcgcttt tttttcagtc
    68521 tcgcggctac tcctgcaccg tccgctcggc gcggttgatc gaccggtccg accctaatcc
    68581 gtaggcctcg gggttctggt agcggccgcg ctcccgcgcc ggtttgtcaa gctcgaccgc
    68641 gatgcggtgg gtctccgcat agggatcgtg ccggatcccg gtcacctgcc atgagacctc
    68701 gatgttgggc ttgtcggtcc tgatgacgaa ccgtccgccg gcgacttttt cggcgatgat
    68761 cgcctgggcg aagtccccga tcacggtgag ctggtagcgg aaatcccggt tgagggcctc
    68821 gaagtagtcg ggcagcgtca caacggcgta gcccgcggca tcggtcgtca ccaccccgtc
    68881 gtagatgttc ttcatatcgg gggactcgac gaaggaatgc tggaggtacc tgttttccgg
    68941 atcgagcggg tggtcgatgc ggaacgaccc ggcagctttg gagagggtgc cggtgacggc
    69001 gacgtcgccg tcgaagtacc ccgcccagtt ggtggcgccg gcggaggcgt aggcatagac
    69061 gccgtaggcg gtttcgccgc cgaggccgaa agcggcgagg ccgcgcgaat agccggcggt
    69121 ggcgtcggcc gccccggcca gggcatagac cccggtccgg tagcccgaat gcgcgaccgg
    69181 cccggaggcg gcggcataca ggctgtggac cgaacccgcg ccgttgttgg cggccccgag
    69241 actcagaccg tagacgaggc cgggactcag aaattccttg ctcacttcga gagccgcgga
    69301 ggcggagggg gcgccggcgc tgccgatttt gacgcctccg acaaacgtct tggcgcccgt
    69361 gactgtctgg gcgccggaga ggctcgcggc cgtcccggcc actttgtcga ccgcgatcgc
    69421 agcggtcggc gagatgtcgg cgtcgacaat ggtgccgtcg gcgatctcgg cggtctgcac
    69481 cgagccgttg gccaggtcga gcgcgctgag ggtgttgtcg agcactttct cggagttcac
    69541 cgagccggcg gccagttccg ccaggcccac cgagttggcc gccagttgcg gatcgggata
    69601 ggtgccggcc aggtcgcccc cggcgggatc gcccagcttc atcgcctggt catagcggat
    69661 ggcatcggag gcgagctgtc ctggggccag gttccggatc aggcgccccg acatatacaa
    69721 atcgccgatc agcgcgcggc tgccgtcgac ccgtacgtac tgggggtggt catcgtcgag
    69781 cagtccggtg aggctgccgt ggtcggaggt cccgcccggc gaggccaggg cgtacgcggc
    69841 ggtgtcggcg cgcacggcgc ggaaagcgaa agccgccgag ccgagtctga tccgcggttg
    69901 tccctccgcc tgaccctcga tcgagagccc gacccagagg ttggtatcgg cgaagaggtc
    69961 cggagtgagc gcgggcatcg gggaaacgcc gaggttgagg gagaagaggc cgccgacagt
    70021 agtgacggtc accacccccg aattccagac ttcgttttcg ggcagggcgg cggtcgggtc
    70081 gttccagata atgatgcgca cgtccgccgg accgtccgct accgggatgc cgaggtcatc
    70141 ggtgagccgg ccctggaacg aaatcacctg gggggcgccg gccatgaggg cggcggcgag
    70201 agtcacgacc gcggccgccg ccacggcggt ggatcgggga attgacatag cttatctcct
    70261 tgcgatttgc tgctctggca aaaccgtcgg gcggcgttgt gcgatgccgg ccaggaacga
    70321 tgcgggaacc tgagaaattc cgccgccttg tgcaagatac taggaagttc aattctgtca
    70381 aggcaacacg gagcgcgcga tgccctctca ccgcacgatc ggcaccgtct gacggcggcc
    70441 gcacacggtc gcaccgagca ggcatgagcc ccggtacacc gtctccccga acctgagcgg
    70501 attgttgacc gtgagcttgt agtaaccctt cggcagatag cgcgccagaa ggggaagcgc
    70561 ggggcggtcg gactcgagga agtgcagggt gaccgacacg aagcaggtgt ccggggcgac
    70621 cttgaactcg agggccggcg cccaggcatt ggggtcgact tcgttggtgc gcacgaacac
    70681 cgagtctttc cggtctgctc gaatcagggt gaaaacggag tcggcgtgga caatgcgcgg
    70741 ctccacccag acctcgtcgt agaaaccgcg cgggacggtg ggctggtcgg ccaccggcgg
    70801 gggcggggcg cacccgatca gcgccgcggc cgcggccagc acgaccgccc ccaggccggc
    70861 gagacagcgg acgagcacgc tcatggcagg taggtgacct cccggcggta ctcgtagtcg
    70921 ccgactttga tccggatggt gtaattgccg cgcggcatgc gctcgggcgg ccgatagggt
    70981 atccagtggg tgccgatgtc gagcaccgtg tccgggagca tgacatcgaa gtgcacgtcg
    71041 cgccggttga ggatctcggc cgccaccggt gcgccatcgg ccgtgaggac cacgccgatg
    71101 cggaaggggt tgtcgggatc gaagggggtg aggtcgctct gcagttccca cagggagtag
    71161 cgggccgtga gattttccca gctgagcgcg ccgcagtagt cgacgcggcc gcggtatttg
    71221 ccctcgggca cgcggaggcc ggagtcgtcg cgtttgtccc agtagaagtt gtagtagccg
    71281 ggggcggcga ggaagtcgac gaggcggcgg acgacccgat tggcggagtc gacgacctcg
    71341 acgatcatgc ggcagcggga ggtgccttcg gcggccgccc agatgcggag gcgttcggcc
    71401 tcggccgagt cggccggtgg aacctgaagc ccctcaatgg tctgcgcgga ggccgccgcg
    71461 cccagcagga gcgccgcgag gcacccggcc ggaattgccc gagtcatctt cataagcaac
    71521 tatatatgta tacacgcgaa ccggctcaag gagaaagatg ttttcgccgt ccgcccaaaa
    71581 gccggtggcg cggcgcggcg ccgatgtcta tactcctgtc cgatggccgc gcgagttgca
    71641 ggtatgatga agttctggga gtccatggcc acagggcgcc gccggggcaa ggtcctgctg
    71701 gcgcttatcg tgctgacgct ggcggtctat ggccacatga ttttcgtctc catcccggcg
    71761 gtttcggccc gggccggagg gatgcagatt ctcgacctga tgccggtcgg gtacagcccg
    71821 gcgtacgccg cgcagttgtt cgagcgactg ggggaggcgg gccgccgcgc ctaccttacc
    71881 gagcagatcc cggtcgacct gctctacccg tttctgttcg gagtgacttt cgcgtggctt
    71941 ctcctctacc tgatgtggcg ggtttcgccg cggcggacat gggccctcgg ggcggcggcg
    72001 atgccgctcc tggcgggcct tttcgactac gcggagaacg ccgcgatcat cgccatgctc
    72061 gcctcctatc cggacttctc ccccggcctg gtgcggtgga gcagcgggtt caccatcgcc
    72121 aagtcgtgtc tcaccacgct ctccgttgtc ctcctgctgg gcgtgctggc ggcggcggcg
    72181 gtgcggcggt tttcccgatc ccgccatcct gcggaggccg catgaaccgc attacggcgg
    72241 ttgaattccg cgcgcggctc gaggccctct gcacccagcg caacaacccg ggtctccccc
    72301 gccggcggcg cgaccaactc atcctgctcg ggagcatcgt gcgggaactg gttccggggc
    72361 gcacctacag cgagcccgac atcaacgcgg tgatcgcggc gtggctggcc gatgtcggcc
    72421 ggtcgatcga tatcgacttc gtgtcgctgc gccggcaact ggtcgatgaa ggcttcctgg
    72481 cgcgcgatcc ggccggcaac gcctaccgcc tggagccgca gcggacggcc gacttgttcg
    72541 aacccgagac ggcggcgatc gacccggcgc aggcagtgcg ggaggcgcgg gagcggcgcg
    72601 agaagcgccg ccaggcgcac cggtgagcgc cgcgatcccc actcaacgag gacggtatca
    72661 caatgaccgg caggcactcc gatctctccg cggcgcacat cgccgccttt cggcgcgaca
    72721 tccgggcctt ctaccgccgc cacggacggc atctgccgtt tcgcgagacg aatgatccat
    72781 acgcgatcac ggtcgcggag atcatgctcc agcagacgac ggtcgagcgc gtgacgccgt
    72841 actacgaggc ctggctccgg cgctttcccg actggcaaac ggtggcggcg gcccccatcc
    72901 gcgccgtcct cgccgcctgg tcggggctcg ggtacaatcg gcgggcgctg tacctgcaga
    72961 agctcgcgca gacggttgtc ggcgagtacg gcggggagct tccctccgac ccggcggtat
    73021 tgcgccgcct tcccggaatc ggaccctaca ccgcgaacgc ggttgcggcg ttcgctttcg
    73081 gccggcgggt ggcggcagtc gacacgaatg tccggaaggt gctgctccac cggttcgggc
    73141 tcgcggcgga tgcgcggccg gccgagatcc agcggatcgc cgagctcgtg ctcccgcgca
    73201 cgggtattcg ggcgtggcac gcggccctca tggactacgc gcgcctcggc ctcccgcccc
    73261 gggagagccg gcgcttgcgg gtgcgccgcc agccgcggtt cgaaggctcg atccggcaga
    73321 ttcgcgggga gatcatccgg cgtctcaccc gccgatcccg agtcagcctg ggcacgcttg
    73381 cccgcgacct ggggcggtcg atggccgagg tgcgggcggc cgccgagggg ctggcccggg
    73441 acggtcttgc ggtggcgacc gggcgcacgg tgcggctgcc ctagggaggg gaaaaccggc
    73501 tccgggcgcc gacttcccgc ttggcatatc cgtgcccgcc cactatattt aaggcgtact
    73561 gcgatatccg ccgatcaagc gattacagga tctgttgcag gagccgttca tgtatttggc
    73621 gaaaccgtca gcccccgtgg cccccttccg cgttctccaa gccctcctgg tttgcctggt
    73681 gctgatcgcc acgattccgc ggccggcggc ggccgccacg atcaacgttc cggccgactc
    73741 ggccacgatc caggcggcta ttctcgggac gctccccggc gacacggtgc tcatcgcccc
    73801 cggcacatac cacgagaaca tctcgttcat cgggcggagc attgtcgtgc ggtcctcggg
    73861 cggccgggag gccacgatca tctacggcga cagcgtcaac gcggcggtcc gtttcgaaac
    73921 gggtgaagac acctcggcgc acctggaggg ggtgacagtg cggcgggggg gcgccgccgg
    73981 cgttctgtgc accggcgcgt cgccgacaat tcacgactgc acaatcgaca gcagcacgat
    74041 cggcatcgac ctggccgcct cgcaggcgat catcgggcag tgcgacattc tgtacaactc
    74101 ctcgcacggc atccggctga ccggatcgct ggcgacggtg acgatgtgca cgctttcgga
    74161 caacggcggg tccgggatct atggcgagaa cgcagcggtt tttacgggca acacgctgcg
    74221 gcgcaacggg agctacccgc tggtgctggg ggcggcctcg atcggtcgcc tcgccgcagg
    74281 caaccagttc atggtcaacg cgattcaggc attcggcgtt ctcggcggca cggtcggcga
    74341 ttcggcggtc tggaaaccgc tgagcggcat ttcaacattc gtgatcctcg gcggcggcga
    74401 cctctcggtc gccggtccgc tcgcgccgac cctcgtgctc gacccgggtg tgcgcctcga
    74461 attcgcggtc gcgaccggga ctctctcggt cggtctgggg aaccccggca cgctgatcgc
    74521 cgatgccccg tcgaccaacc cgatcagctt cgtcccgcac gcctcgatcg cgcagtggcg
    74581 ggggatccgc ttcgagagca acgcccgcga cgaggaatgc ctgctgcgca actgcctcgt
    74641 ccgccgcagc caggggatcg tctgcaactt ctcctccccc gtgttcgaca gctgccggat
    74701 cgacagttgt gcgggagacg gtctctcgct catcggctcg caatcggtca tcggcaattg
    74761 cgtgattgcc gataacagcg gcgtcggtat tcgcctgagc tcctcgcagg tcccggtcac
    74821 cggctgcgag atctccggca acggcagtta cggcgtctac gggaccatgc tcacggcgtt
    74881 ttcggggaat gtcgtgtcgg gcaatgctgc cgccccgctg gctgtcggcc cgacctcggc
    74941 cggccgcgtc gacgccacca acagcctcgc cggaaacggc gaccccacga tttacgtgct
    75001 ccccggcacg gtgtcggcct cgggcacgtg gcgggcgcac ccgggaattg agcggtatgt
    75061 ggtggcgagc acggcggccg tcaacgtgca gggggcggcg acgccggtgc tgacgctggc
    75121 ggcgggggtg cggctggagt tcggacaccc ctcctacggg ctgattgtcg gcgcggcggc
    75181 ccccggcggg ctcatcgccg aggggacaga ggaactgccg gtgctgctgg taccggcggg
    75241 tccggccgcc tcctggggcg ggttggcctt cggcgcctcg gctgtcgact cgctgtgccg
    75301 cctcgggcac acggtcatcc ggcgcgccca gaccgtcgcc tgcaccgacg cctccccggt
    75361 cctggacagt tgccggatcg acagctgtct cggcgacggg ctggcgctcg tcaacagcca
    75421 ggcgcaactg accggctgca cgatcgccga taacggcggc gacggcatca cgatcagcgg
    75481 gctgccaccg ggtatttccg gctgcgcgat cacgggcaac gccgagaacg gcgtagtggg
    75541 cgggccgctg gaggtgtttg cgggcaacac gatcggcttc aacggcggcc acgcgctctg
    75601 gctggaaccc aacagcgtgg gccggatcgc cgccgacaac cttttctcaa gcccggacca
    75661 gacgatcgcc gtgcagggcg ggatcgtggg cgagtccgcc gtctggcggg ccttccccaa
    75721 tgtccggcgc tacctcgccg ggacctcggg gatcgtctac gtgcagggag ccggcactcc
    75781 ggtgctgacc ctctcccccg gccttcgcct ggaatttccc tccgcctcgt tcgggctgcg
    75841 cgtcgggtac accgccgcgg gcgggttgaa ggccgacgcc ctgggcggcg agccgattgt
    75901 cctgacccgc gacaccaaca ccgcctcgtg ggccggcctc cgcttcagcg accagacgat
    75961 cgactcggcg tcgtatctga aaaactgcat ctaccgttcg gcgcgcagtc tctatgtcgc
    76021 ggacgcctcg ccgatgatca cggactgcac gatcgacagc tgcaccgagt ccgggctgac
    76081 gctgaccaac agcgtgtcgc gggtgctccg ctcccggatc acggacaacg ccgtgcacgg
    76141 cgtctccgtc agcggcggga cgccctacct ggccagcagt ctgatcagcg gcaacgccca
    76201 gtgcggtgtg tattgcgcca atgacgccaa gccggtcatc ggttcggtcg ccggagcggg
    76261 ctgcaacctg tcgcggaacg gcacgttcgc gctgtacaac gacggcgcgc agacgatcta
    76321 cgcccgcgag aacgcctggg gggacgacga ttccgcgggg gtcgcggcgc tcatctggga
    76381 ccaggtcgat gatccctcca agggttgggt gcagttcatg ccgttcgtgc tgggctatgt
    76441 ctgcggagac gcgaacggcg acggggtcgg gaacccgggc gtggccgacg tgacctacct
    76501 agtcgcctat ctgttccggg gcggcccgcc tccgccggag atagacgcgg ccgacgtcaa
    76561 caacgacggg gcggtgcgaa tctccgacct ggcctacctg atcgcctttc tcttccgctc
    76621 cggcccggcc ccgtactgct gggagtacgg tccgagctga gctttcggtg tcggccggcc
    76681 ccgaccgacc tgtgcaattt tccaccggca ccgcttttcc tgcgcctttc cgggaagatg
    76741 cgcggctctc cggtgtaatc ccctcgaaga cagcgatcaa ccgccctagt taaggaggtg
    76801 gcgtatgcgc cgcacaggac tctttctgct gtttgcgatc ctcaccccga tgtggattgc
    76861 cgcatccggc gagcgaaaac caaccgccac aggaaccatc accggtaccg tcaccgacaa
    76921 ggagacgggc cggccggtga ccagtgcctc ggtgctggtc gtcgggaccg ccttcggcgc
    76981 ccagaccgat ctggacggcc gctaccggat cgacaacgtc gcgccgggtt cccacctcct
    77041 gcgtttcagc cacgtcgagt accgtactgt cgagctggcg ggcgtcgagg tggctgaagg
    77101 atcgaccgcc gtgcggtcgg tggccctgga gaagaacggc gcggtcaaaa gcgacgtgcg
    77161 gatcctcggc acgaaggaca agctcggcgt cccggagagc agcagccagg ctccggtgac
    77221 caaggaggaa atcccgatgt accggttgcc ggcagaggat caggcgctcc ggcaacctcc
    77281 ggatgcggcc gccgccggcc acggggttga gatcttcatg cgcggcggca gggcgggcga
    77341 ggcggagtac attacgaggg agaattcgct cccgccggcg cacggcggct cggcgatcgt
    77401 caacgggcag ccctacgacg ccatgttttt ccgcgactac ggcgtcaacc cgttcgtcga
    77461 caccgatgac gaccatctct cgacctttgc cgtcgatgtc gatgacgcct cctacatcat
    77521 gacccgctcg tatcttcagg acggccacct tccgccggcc gaggcggtgc ggacggagga
    77581 gttcatcaac cacttcacct acgactacga tcctccgcgg cgcgagactt tccgcgtcta
    77641 tgccgacggg gcgccctcgc tgttcggcga gaagacgcgc ctcttgcgga tcggcatcaa
    77701 gggacgagag atcgatcctg aggagcggaa gccggcgacc ttggtgttcg tgatcgacgt
    77761 ctccgggtcg atggcgcggg agaaccggct cggcctggtg aagcagtcgc tgcgctacct
    77821 ggtcgaccag ctcctgccgg acgatcgggt cggtatcgtc gtgtacggct cctcgggccg
    77881 ggtgctgctc gagccgacgg gagcgggcca ccggaccagg atcctgcgcg tgatcgacgg
    77941 cctcgagccg ggcggttcga ccaatgcgga agagggcctc cgcctggggt atcggatggc
    78001 cgaccgcaac ttcgtgcgcg gacagatcaa ccggattatt ctgtgctccg acggcgtggc
    78061 caatgtcggc aagaccggtc cggaggagat tctccgcgac attcggcgct acgccgaacg
    78121 gggaatcacg ctgacggcgg tcgggttcgg catgggcaac tacaacgacg tgctcatgga
    78181 gcagctcggc aacaaggggg acgggttcta cgcctatgtc gacgatgcgg ccgaggcgcg
    78241 ccgcgtgttc atcgacaacc tgaccggcaa cctgcaggtg atcgcgcgcg acgtgaagat
    78301 tcaggtggag ttcgacccgc gtgcggtccg cagctaccgt ctgctcgggt acgagaaccg
    78361 cgacgtggcc gaccgcgatt tccgcaacga tgcggtggac ggcggcgagg tcggcgccgg
    78421 gcaccaggtg acggcgctgt atgagatcaa gctgtggagc gacgggaacc gccgcgacaa
    78481 acacgccatc atcgaccggc gcgaggacga tgacggcgcc cggccgggcg atctcggcat
    78541 cgtgcggatc cgctacaagg atccggaccg cgaccgcgtg gcggagatca ccgggcgcat
    78601 caccgacgag gtgttcgacc gcgcgttcgc tgaggccgac cccgatttcc ggctggccgc
    78661 cgcggccgcc gagttcgccg agatcctgcg ccactcctac tgggcgcggg actcgcggct
    78721 gtccgatgtg attcgcgtgg cgcaccgcgt ccggagcgag accggatcgg aagaagcggc
    78781 cgaactggcc gacctcattg cgcgcgccat gcactatgaa gacgagctcg ccgagcgctg
    78841 agcgcgggag cagctctttc gagggcgcgg ccgaacgagg gatcggccgc gctttttctt
    78901 ggcgcgccgc gcgggcgccg gcggaggatc cggcggaagc ggcttgcccc gccgttccgt
    78961 tctgcctata ctgtcgcgct gtggagaatc tcgcgggaca tgacagaacg cggcgcgcgg
    79021 ccgcagccgg cgtggcagag cgcttcgccg cgcgctaccg gggggcggtc gacgattacc
    79081 cggctttcct cgcgaccctg acccgtccgc cccgcaacac cttccgcgta aatacgctca
    79141 aggccgacgt cggggcgacg gtcgctcggc tgcgggccat cggattgaca atcacacctc
    79201 tgccgtggtt cgaggcggcc tttgcggccg acccgcacat caacctcgcc gccacgctcg
    79261 agcgcttcct ggggaccatc tacatccagg aggccgcctc gatgcttccc ccgctggtcg
    79321 ccgccgggga gctgcgcggc gcccggacgg tgctcgacgc ctgcgccgcg ccgggttcaa
    79381 aaaccacgct cgccgcggcg atcatggcca accgcaacgt cattgtcgcc aacgaccgaa
    79441 gctacggccg gatccgcgcg ctccagttca atgtccacaa gttcggggcg atcaacaccc
    79501 tcatcaccaa ctacgaactg cagctctttc cgaaagcgtc gttcgacacc gtcctcctcg
    79561 acgccccctg ctcgtcgagc gggacggtcc gcaagagccc gaaagtgctc gagacctgga
    79621 cgagcgccgt ctccgccggc tacgccgagc ggcagaagga tctgatcctg cgggcgttcg
    79681 acctgcttca tcccggaggc acgctcgtct attcgacgtg ttcgctggcg cccgaggaga
    79741 acgaaggagt cgtggatttc ctcctgcgca gccggccggc cgagacggtt ccggcggcgc
    79801 tgccggggtt ccgcttcgcg ccgggtctcg cggagtggga ggggcggcgg tacctcgcgc
    79861 ctgtcgcccg ctgcgggcgg gtctggccgc acgccaacga taccgacgga tttttcgtgg
    79921 cgaaggtggt gcgatgctga ccccctgccc cgacgaaatc cgccggcaac tgctcgcctg
    79981 cctggccgag cgtttcgggt tcgacacggc gctcctcgaa ccgtacgagt tgtacctgtc
    80041 ggaatcggcg cggcagaaga tctttctcgg gccgaagact gatttcccgc tcgcgcggac
    80101 cgacaccggc ggcgtgctca tcgcccgcgc cggagcgacc atcaaacccg cgacgagctt
    80161 cttccagttg ttcggccgcc acgtgacccg caacatcata catctggagc gcgggcaggt
    80221 ggcgcccttc tgctccggaa gcgacctcga gctggcggag aatcagatcg ggggcgccga
    80281 gcgggggttt gtgatggtgg tgtgggagga gcactgtctc ggctgcggac tcctcaaagg
    80341 gcggacgctc gagaaccaga ttcccaaagc caaccggctt accttggagc agtggtagcg
    80401 ggcggtcgcc catggtggcg ggccacccgc ccggccatga tctcctgcag gtcaggaccc
    80461 ttgtgttcct gacattcctg ccaacgacgt aagcggtccc gtcacgaccc ttgtggtcct
    80521 gaccgacaca acatccttcg tgggaactat ccgccctgcg ctacggacgg ctctgctcgg
    80581 ggctcccggc gggctggtcc gctgcatttg cgtccccatc tcccccgccc aggctgagca
    80641 tgtagtccgc caccagctgc agtttttcgt cgccgaggtg ggccatcgaa ggcataggct
    80701 gaaattgcgg gcgtttctta ccggggttct tcgcccactg gacgatgccc gccgggttgt
    80761 tggcgtagag cgagtagatc tctttgatgg acggcgcggc gaggaccttg tcgagcgcat
    80821 gacaggcggc gcagtttgag aagacgctcg gcccggtcgg agcgccgccg accgtctcgc
    80881 cgacccccag cccggccgcc agacgcatcc gggccgccaa ctccaccgcg gcgaaagtag
    80941 cggtgcggtc ggcgaccgcg agccggtgcc ggctgagggc ggtctcgcgg tagagatggc
    81001 ggccggtcgc catgaagagc gcaaggacgg tgaacagggc gatgaccgcg ccgtagcggc
    81061 gcccgatcgc cggattgccc gcgcgggtct cccgccagag cagggccaga atgagaacga
    81121 cgaaaaccgc agcgacgccg acgacggcga gcagagtcca actgatgcca cgcagcggga
    81181 gcgtgagcag cagcagaggc ccaaagacga gttgcgccgc cgtcgcccag aaagcgaggc
    81241 ggtagaagag accgcgcaac tcggcccggg taaaagcggg aagcagctgt tcgaccggga
    81301 atcgccggcg gccgaaccag cccgcgagaa acaggccggt gacggcgagc gaggcggtca
    81361 ggaaatggaa gtagcgcggg aagacgttgc cgtactgcag cgacgaaaag aacccgcgca
    81421 ccgcggacca ttggtcggga aagagcatca ggttgatgtt ggccaggaag atgagcggga
    81481 tcatcaggaa gaagaacgcc ccggcggccc cgatcacgat gtgatggttc ttcgccggcc
    81541 cggtccagcg ctcccaggtg tacttgtgga ggtagctcag gaggaacgcg gtgatgacca
    81601 acgggacgag cgaaatccag gcatagccgg tgagcgcgtt ggccgagtag aagtagagcg
    81661 tatagagcag gttgatgcac aggagcggac cgacgccgag caccacggcg aggctcttgc
    81721 tgacggtgat ggtggcgccg atccgccgcg cgagggcatc gtaacgggga tgggagcggc
    81781 cgacaatttc gcacacgacc gtcagcagcg acccgccgac catcagattg acgaacacga
    81841 tgtgcaggag aaacaggacg accaggaaca cttcaatcac ggcggggtcg agcggcagag
    81901 gcaacgacag atcgcgcggg acgcgggcgg caaggtctag tggagccatc gtcatcacct
    81961 cccctcactg ccccggcccg gccgcggcgg ccgcggcccg cggggaatac ccgaccgcct
    82021 gcgcgcccgg cagaccgcgc ggccgttcct gcaggtctct cagataggcc gccagggcat
    82081 ccgcctcggc cggggcgccg ggaaacggcg gcatgaaggt gcgggtgttg tgcatgacgg
    82141 cgatgaagga actcaggacg gccggatccc agggcgtgtc tccgtagagg ttgcggaaac
    82201 gggtcaggac gctgttgacc ccgctcaccg tgtggcagcg ggagcaggcg agcatgtaca
    82261 catcgcggcc ggcgtcaacc ttgttggccg aagtgacctg gcgaacggtg ctgtaggcgg
    82321 agtacgtcaa aatcccgtcg cgctggaaaa tcggcagctc cgacatggtc accccgttgg
    82381 agtacatgta gtcggcgaca acgtggggtt tgcgaatgaa ctcgcgcacg cgctcgaagt
    82441 ggccgagcgt gtagagaccg agcaggaagg ggatgacgag cacggcccgg ggaatcaggg
    82501 ccgggcgggc caggccggcc agcgcgatca ggacggtgag ggcgagcacg gccgcgacga
    82561 catagaggaa agtatcgtac cactgggtca ggcgctgggt aaggaaagcc acatcgaggt
    82621 tggccgccat cggcgcgggc acgcgccgcc agtaccagac agccccgatg agaacgacag
    82681 gagtccaggc gagcacccac ccggacacga tgcgcactac ccgccggcga agttcggggc
    82741 gggtccgggt gaagaagaag gtcaggaacc agaccgtgaa gcccgcggtg accatggcca
    82801 gcccggtgcg gaacaccagc tgggggaggt agagcgggtt gaagacggcc gagaagaagc
    82861 tgcggtcggc cgcccaggac ccggtcccca tcatgaaacc gaggatggcg acgatgatgg
    82921 ccatggtgag ccaggagccg acggcgagga cggcgcccac ggcgttgtgc agcgctttcc
    82981 aacggccttc gcgccagcgc tgccacatca ggaagtacac gatgaccagg ccgacctcgc
    83041 tgatgaacac cagccactcg atgaaccagg cccagaagaa gacgcggatg aggctgccga
    83101 tgccgaaggg ggcgatgaga gcggcggtga gccagatccc gaccccggtg agagcgccga
    83161 aggtcgtggt gatgatgaac aggacgaagg tgattttgta ggcgagagca tcgaggtcga
    83221 ggtcgccgcg gcggcgtccc caccactcca aaagcgcgac cagcggccag gccccgaccg
    83281 ccatgggatg gttgatcatg acgtgggtga tcgccaccgc cgccatgagc aggcgattgc
    83341 cgatgaaatc cagatagaac agcggaaaat ccatggctcc tcgcacgctg cggcggcggg
    83401 aacgcccgcc cgccggttcc cacgataccg gccgccgccc cggccttaag cgccggccgc
    83461 acgaggactc aaaggtacgc aacgcgggct gtgccgtcaa ttattaatcg gaaccgaccc
    83521 gccggcgaac gatgcgcggt tgcaccggcc ggggatttgc gtatattcca ccgctatgac
    83581 gtacccgttg ctcacgctca aagccgggcg cgaaaacgcc ccggccaacc gccatccatg
    83641 gatcttctcc ggcgccctcg ccgagggcaa accggaggtc ccccacggcg cgctcgtccg
    83701 ggtagccgag gcctcgggcc aggtcattgc gacgggaacg tactcggcca aggggatgat
    83761 cgcggtgcgc gtgctggcgt gggaggaagc gcccatcacg cgggagtgga tccgcgagcg
    83821 ggtgcgggag gcggtcgagc ggcgaaggct ggtcggaatc ggcgcagacg agacagtcac
    83881 cggataccgg gcggtgttcg gggaggccga cgggctgccg gggctggtcg tcgatcggta
    83941 cggggaggtg gcggtcgtgc agttcgcgac ggccggcgcg gagggactgc gggagccgat
    84001 cctgcaggcg ctggccgacg tgctgacgcc ggcggcgatc tacgagcgga gcgatctccc
    84061 cgcgcgcacc gaggaaggcc tcgcgccggc cagcgggctg ctctccggag tgcttccgga
    84121 aagagtggag tttgcggaac acgggctcag gtttacggcc gacgtgacgg gcgggcagaa
    84181 gacggggttt ttcctcgatc agagagatgt gcggcggcag gcgcgggcgc tggcggccgg
    84241 gcggagagtg gccgacctgt tttcctaccg gggagcggcg ggcgtcgcgg ccctgaaagg
    84301 cggggccgag cacgtgcact gcgtggattc ctcggcggcg gcgcttgccg ccgtggggcc
    84361 ggaggcggag gccaacgggc tggcggcggc gcgcatcacg gctgaagagg ccgacgtttt
    84421 ccagtggctc ggtcagcggt cggagccgga gttcgacatg gtgctgctcg atccgccggc
    84481 gctgatcaag tcgcgccggc atgccgagga ggggcgcaag gggtaccact tcctcaaccg
    84541 ggcggcgctg cggatcctgc gcgatcaggg ccttctcgtg acctcgagct gttcgttttt
    84601 cttcaccgag gatgacttcg ccacgaccct gcggcgggcg gccgcccagg cgggggtcca
    84661 gcttcacgtg ctggccacgg tgcggcaggc ggcggatcat cccgtggccc tgcattttcc
    84721 cgagtcgttt tacctgaagt cgttcgtgtg ccaggtgcgg cggcgagttt agtgttgcgg
    84781 gatcaggcgg gggcgggtat tttggtgtag aatgatcgga cgcccccgta gctcatccgg
    84841 atagagcatc tgccttctaa gcagagggta gcaggttcga gtcctgccgg gggtatttaa
    84901 tcttaagccc tccaacgact tccagcgact actccaacac aaaacatgag gccaaaacag
    84961 gcaaaagggc ctctgactag taggagaagt catggaagat catctcaagc gactctacct
    85021 tttggtcgat gaactgaagg tggaatctca ctggaagaca tacgtcgatg cgtttgtctt
    85081 cggttgcaag ataagaaatc tggcacctcg cacactcaac gtctatgcgg agcggctcgg
    85141 ttacctggtc cgctacctcg aagaaaaagg catcgacatc gaggatatta cgcggcagaa
    85201 tatccaggac tacctgctgt cgct
//
LOCUS       DAOPOE010000003        78550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_388_length_78550_cov_5.951131, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000003 DAOPOE010000000
VERSION     DAOPOE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 78550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78550
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_78550_cov_5.951131"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="PKM94_00815"
     CDS             <1..2413
                     /locus_tag="PKM94_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD65206.1"
                     /translation="GGYAGTEGFTGGIAFDPQRNLLYFASRAYSGDFPTDGVFYRVAA
                     GTGMLAGPALAATQTVYSGPTVDANRVYVAGNSRYVAAPFGGQIYAVDKATGVRVWAA
                     NAGPWGYIISPPAPTTASLDGCYNSAVLSCEPTGVADLLFAFGSFGGYYVYNAETGDN
                     LYYNRVSVGTNPVAQRGFGGALQVDDDGASHLLFADNSGRLYDLTKQGDRPRLEIQHY
                     DMKVPCPFGTDPNYLLTIPDVYTNTGCADLHFTNVIVDEISVANQWIPDIVAQYVPDD
                     VMMKAAGIADRLTDFQFSGKFLREADATEDVVIASDDLERRTNYAAAGVPAWFNSMLH
                     PAISDVLIAGDTMDLQFYVNQAMILRGAQDFYLQVDSDDPEFFLNDVNKLPEQHVVVV
                     GGCLTDTTTLIFGVGGANERLVSNTGRLATGDWGDGPAGYNGITIDGDVSSYFQGSFA
                     FMGSKYQVAANTDDWGGGGTAMVSMQGDPNWCDDDCKPALQTGVTLGYITGDGGLTYD
                     PILGNRVCYTILDSVQNFDAGTGWDWTWYDAPFDNALTMGLYANCRAVGALDVPELAN
                     MTIDVMEITERNGNAVDGWYLSHFFDCDNGSDTIQYNGTYSVAWTLDGGAAQAIGNVK
                     IPFGCGYTPIVNIWGTYGVSGSHGFWDWTQYWDTAYWYCTQGYGAKTEATGMHGGDEE
                     AHVTLDQHNFAPNETYTVGIATFELFGLTNSLDANNPDIIALAKIANQWAGFGRGDVN
                     NDGAINLADIIYLAGTVNGGPGAIPFQHLADVNADGNIDVVDVDFLIDYYFNCGPCPT
                     GDWIF"
     gene            2511..3605
                     /locus_tag="PKM94_00820"
     CDS             2511..3605
                     /locus_tag="PKM94_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD65207.1"
                     /translation="MDPRDFDSETEAISPLLRDLYEINAVYRQGEELAFSLSWRYAPV
                     KSRKLLEDRLRTAGYRYRLAEGDQLTLVVDPRRRLRIPPLNIILFAATVLSVYLLPVL
                     LANAGAPHLWAATLADLRRGAGLQFTAALMSILLVHEMGHFIASRRRHIVTSWPYFLP
                     APNLLGTFGAVIKSKSPFYNRRDLVEVGAAGPIAGWVVALGWLIFGLTQSRWIPAGLV
                     VPGEWAFGLEGESLLMRAAVQALVGAPAPEHFLKLSEGAFAGWVGLLVTAMNLLPIGQ
                     FDGGHVLYGLFRRAQHVLGMIGLAALLVLGYFSPFWWLFGAFGLLFGVKHPPTLDDDR
                     PLSPTARRMGIAAIVIMALSFTPMPFPSPF"
     gene            complement(3574..5100)
                     /locus_tag="PKM94_00825"
     CDS             complement(3574..5100)
                     /locus_tag="PKM94_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65208.1"
                     /translation="MAERPTTHIVVIDDEQYICNIIEEALAAPEFQVAAFSDPLAALA
                     HIDDHPVDLVLTDLVMGDHSGTQVLEATLTRHPDAVVILMTAHPTVQTAISVLRRGAY
                     DFLVKPFKLELLKSTIERALEHQRLARENVRLRGQVEFLKVANAHKAHIDIERYLRLV
                     IESCKKELGAVAAGIIEVDPSSGDLLRKLCVVDDPLLEPLALDPTVLEKFSSAGRAEP
                     VIEARRTQSKAGREVRLSILQPIMVRQRFHGVITLLVVSKFGRVTPGQLDVLAILTNS
                     AAAAIANDKLYRDLRRSYLEAIRGLANAIEARDKYTAGHTDRVSRLAEMIARRLGWSE
                     SRIFDLHMGCTLHDIGKIGVPDRILNKPTALTEEERTQMRSHPEVGLRIIRGIELFKP
                     AVPYIKAHHEWYDGTGYPKGLRGEEIPVEGRLLAVADTFDAILSDRPYRNGNSLAVAV
                     RELLTYRGRQFDPEIVDVFLEILREGQIDFRRMYGRSEDVSCLIDEARAKREKETASA
                     "
     gene            complement(5216..6094)
                     /gene="lipA"
                     /locus_tag="PKM94_00830"
     CDS             complement(5216..6094)
                     /gene="lipA"
                     /locus_tag="PKM94_00830"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOD65209.1"
                     /translation="MADSPPSRPPGGGPRKPAWLRVTAFSGMGYENVSRLLRHYGLNT
                     VCHAANCPNRGECFNRGTATFLIMGPTCTRNCRFCDIEPGRPRPLDPTEPARVAAAAA
                     DLQLRHVVVTSVTRDDLPDGGAGHFADVVAALRARLPAAAVEILTPDFRGVAGAVDII
                     IAARPDVFNHNVETVPRLYPAVRPGADYARSLALLAEVGRRSPIAVKSGLMVGLGETR
                     EELRTVFHDLAGAGVRLLTIGQYLAPSAAHHPVVRYLPPGEFAELRQEAEAAGIPLVA
                     AAPLVRSSYRADEFCR"
     gene            6189..8279
                     /locus_tag="PKM94_00835"
     CDS             6189..8279
                     /locus_tag="PKM94_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD65210.1"
                     /translation="MRRLLVVVGCLLLPVLASSPRAERGQARLVPVPDGAARAELRPV
                     PSLATADTCIVRHDAGIYYKIDGWVTGNELYKGYCDPALTCPNPYPFIVTEINMPMYF
                     TGTPTFGVSVDVELADNSDPSCPMPGPLVAISSEYTLQVPAEGLYDIWIPLDTPVTVT
                     GPFFAGFFLSTPFAEADGAAVVIDTIPELCVSYNIWDTAVGFIDLGDNEIWNFPGRLV
                     LYAAGVPIGTANPPDVRLLSPRPSDTLFGPTEIWIQEKGGSPILDYAVFEYRPAGGGA
                     YVEIGRDFDPTAAFRDGASATAASAAGLSYAFNFGALTEGMWTLRATVYDTLGRFDRD
                     SVTVYLEPTPPVPTVISHEDGGSFCTPLTFNLVCSDEDVDYTQGFRRGALDEYSAGVI
                     VAGPPARGPHWNAPYAAAAAIELWYDRGTTRPMTENNLKLSLDTLAARLAYWMETTVN
                     TGTWDEGFLAGLNEYLHVKGDDLYTAYRRRPNYFQIRDWAENWERSVLLGLAGTPGQW
                     VVVDGFSGWLQVDGTYLVRIMDPLTGTAAPVSLRHNMGTAEVFVGGAWRPVDLMVSVA
                     TKAWNPTRYAIGADLNGSNGWSISWTPSGLTEDDLYFFDLAARDGHNMRGVTTLLLQY
                     NCAGNYAKGDYNNDGATDIIDADYLLRFVVSGGSAPIGGAGRGDANCDGYVNFADVVF
                     FMNYLFGGASEPCY"
     gene            complement(8376..10751)
                     /locus_tag="PKM94_00840"
     CDS             complement(8376..10751)
                     /locus_tag="PKM94_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD65211.1"
                     /translation="MKLSYRAARFSHRLVLLAATSLLATTAPTVRAERASSSEMSRAC
                     ENWLTQVVFTRGAWAGAAAPTVLGAHELAVGDTLLARYYDISPRGFVLVAALKEMTPV
                     KAYSDRYNLDDSQSGGFLAMITEQLANRTRLYAEMFGSLEAAQPPEGPALFGRGQKAE
                     WTRLTLPAGEFGADLAAQAARATRDGGPLCTTSWDQGYPYNKFCPQGQYGTSVVGCVA
                     TAASQVIAYWQWPPSGVGSHTYYWDGDQYCGGNYGGGYLTASYSDSYDWANIVDSCSL
                     GCTPAQEDAVAELCYEVGVAFDMDYSSCGSAAYTGQAVLIYPQYFKFSPDVRRVDRSA
                     YSLHEWFDLVKNEIDSGRVVQYRINLHSIVLDGYREQIPGRYEYHMNYGWADAFNAWY
                     VFDSLYCGWIEGDICPASEEYMITHIFPQTQPVLQKVGEEIIEAGGDGNGRPNPGEAV
                     DLNIQVVNNGWDAVNPAVTLSTDDPYIAIEAASASLGDTLAWGETGFTQTPLKLAVDA
                     GCPDPHVAVLHVTMAAEGGHTASDSIRIFIGNSAGLASDFEVDDNRWWHRPHTQFFGD
                     QWHRETYRKHSGATSWKVGGTGGNYYDNALDAALTSPPFLLPPNAVLSFWHWRNIESP
                     TDAWDGAIVMISVAGGSWQQVFPEGGYPDTIVDNPANALPGGTPCYSGDAEWSEAVFD
                     LSAYAGVAQVMFRFASDGALTYEGWYVDDVAIVSTGCCLGLRGNVDGDAEDEVGVSDL
                     TALVAFLFRGGPAPACDEEANIDGSGILNVADLTTLINYLFRGGPPPAACP"
     gene            complement(10968..11058)
                     /locus_tag="PKM94_00845"
     tRNA            complement(10968..11058)
                     /locus_tag="PKM94_00845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11022..11024),aa:Ser,seq:gct)
     gene            complement(11255..12343)
                     /locus_tag="PKM94_00850"
     CDS             complement(11255..12343)
                     /locus_tag="PKM94_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD65212.1"
                     /translation="MAMTQGYIVGRPAAAIARPPTAPPHLAAAAPADRRPQPQPATLR
                     AGRDGSLSMALPAFAARTTVFRWLRDYFLGLRLLVRSLPRFASLTVTYHSDLSFVDQV
                     APVLLLAKFFGVRTTVLYRSNKAETELDDYARAIIPFLRLSDRVVVSCAYLAEVFSRY
                     GLTAAVRADTVDAELFPAKQVTAVQPHIIMTRRLAPGNGLTGALKAFHLVKQKYPRAE
                     LTIVGDGPLRPWLEEFVRRERIYGVTFTGRVDHAEVARRMAAADIYLNNATIDALPTS
                     LLEALACGRCVVSTAVGEIPRVICDGRNGLLVAPNNFAGLADRLLELIETPALCARLS
                     RHAPASLAQWAASANAPDPATPDPRTDP"
     gene            12556..13650
                     /locus_tag="PKM94_00855"
     CDS             12556..13650
                     /locus_tag="PKM94_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD65213.1"
                     /translation="MVKKVFLITFIVVIVGGVLFFAIDGSAKKEEGLKTVPVERGDII
                     DKALAVGRIVPEKEIAVKSKISGIVKRRYVDVGETVAVGDPLFDIAPDPTPLEFAESK
                     RQVELAQVTFDNAKREFDRTQSLKDKQLISNQEFDKARAAYEEAELRLKLAEERLSLI
                     QSGRTTVADLQVDNTIKAPISGTVLSILVEEGDPVVPLTSYQAGTELMTIARMDNLVF
                     KGDVDEIDVGKLHEDMGAEIEVGALPNSKVTGRLTKISPKASQKEGSTLFEVEIALGE
                     VSDTFLRAGYSANADIIITKKEGILTVPERLVKTEDSVSTVEVQDTLGVVTKREVKTG
                     LSDGIKIEITDGLEEGELLVERPPREITGD"
     gene            13668..14903
                     /locus_tag="PKM94_00860"
     CDS             13668..14903
                     /locus_tag="PKM94_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD65214.1"
                     /translation="MVPLQVFRQFMNDVRRQKLRSALTMFGIFWGTCAIVLLFAFGQG
                     IGEAQIKSQRGLGEDIAIFWPGITSKEYRGLPRGRRIRPTEDDVLLIKQKAQAVQRIS
                     PEYSRWNAPMKYGRNETVRNIVGVWPEFGEMRNLIADVGSRFLNGSDMAERRRVVFLG
                     NRLKTDLFGDQDAVGRVVLINNVPFTVVGVMKSKKQDSSYNGRDDSKAFIPASTFRAM
                     FSIRYLNDFVVQSYPDRTMKEAREEIYGILGARYRFDPSDTEALMVWDTTEGMAFLKT
                     FFLAFQAFLVGIGAATLITGGIGVSNIMNVVLEERTKEIGIKMALGARKSLIVSQFVM
                     ETLLITGFGGLCGFLFAAAIIAAVPMFQVADYIGTPRIDWFGSLVATLLLGLVGFIAG
                     IFPARRAANLQPVQALKLF"
     gene            14912..16162
                     /locus_tag="PKM94_00865"
     CDS             14912..16162
                     /locus_tag="PKM94_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD65215.1"
                     /translation="MINLATVYNVFIRDFRKQKKRITLTLIALGWGTISIVLLLAFGE
                     GLQRQMSINGKGLGEGIAIVWGGQTTIPFKGYGKGRPIWLKKDDVRYLEERVPGLRYI
                     GGEYHRWGTEIRYGDKVLSEHANGIPPNYEFMRSHIPMQGGRMINDLDMRLKRRVAYL
                     GHNLKERLFGDEDAVGKQILVKGIPFTIVGVEIEKMQMSSYSGQDADKLSLPATTFEA
                     IFGDRYLDNIVYQPERVEDMKAVEKAVYEAMAAKYTFDPADERALWVWDVAESARQFE
                     NLMIGLKLFLGIVGGLTLLIAGVGVANIMYVSIRERTREIGIKMAVGARRSYILAQFL
                     IEAIIITFFGGFGGLAISYILTEGFKRLPIESEVLDFMGRPTISLDVGAIVVAILGVM
                     GLLSGFFPALRAASVNPVESLRYE"
     gene            16190..16900
                     /locus_tag="PKM94_00870"
     CDS             16190..16900
                     /locus_tag="PKM94_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65216.1"
                     /translation="MLIALHNVEKEYRTGAVSFKALKGVSLAVDRGEYLAIVGPSGSG
                     KSTLMNLIGCLDVPSAGEYLLDGRPVGAMTSNQLADIRNQTIGFVFQAFNLLPYATSF
                     ENVEVPLLFARVPARQRKRRVLELLARVGLADKVSNKPTELSGGEMQRVAIARALANE
                     PEIILADEPTGNLDTASGKQIIALFDELRNAGKTVIIITHDVNIAKQAPRIVRIRDGA
                     IEENGYHPVGVNVQSLQG"
     gene            complement(16975..17622)
                     /locus_tag="PKM94_00875"
     CDS             complement(16975..17622)
                     /locus_tag="PKM94_00875"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD65217.1"
                     /translation="MISWREIGRLPNLLSLSRIAMTPLIGYFLWLDTPAAPFVCVGFL
                     FLAAVTDGLDGYLARRRGLTSDLGRVLDPVADKIMAGVLVLLLVAYRGFPLWLAVVIL
                     GRDLLIMAAGLFLLQGRKVVLGSNITGKYTFTAIAFLLGSYVIRFPFGMLVMPPITLA
                     LIAFSMILYARVFLRVRRGDRPAPEHDRPGYKMVRVTLLVLFLVIFAVQLAEYLW"
     gene            17992..19467
                     /locus_tag="PKM94_00880"
     CDS             17992..19467
                     /locus_tag="PKM94_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65218.1"
                     /translation="MIVPRILAAAILGVLLLAAPQAAAQLCGDVDNSGGVSLGDLAAL
                     VNWLGIEGAPPLPNPAMAEMDGRAGITVGDLVPLLEYVFEGGPVPTCGATDTYTFAPS
                     PGDTLFIPRMLGIAAEVNEVTLPLTIHVQSGVRAFNLSFLENTAGPAFRLADVTYPPE
                     VGLLLVGRDYTNHDSCRAIGLEFNPNPFTGTIVVANLIYERYSSGVADIIPVPVNRAD
                     PYRNAVARDGDLYVPTVAYLDAARPPDTLFAAPTALDFDAVYGYPAVDTLPVSFTSAP
                     AAVDFTISPDESWIRVVNGAESPWTAPATVQVTVDLAGLPGGLHTGHLNISTTDPEVI
                     LPASAIEVNLTIQAPVDTLFVSTDSVRLEAVAGVPSYPIVYVNFSHSLNPLQFNLLPG
                     QSWVKLADPAAAPFTSPATVGLTVNHTGLPIGEYVGEMLVVPAAPGVYVEHSPIRMYL
                     KVTGLALPPGDLNCDGAVAVADVTMLINYLFRNGPPLTPCQ"
     gene            complement(19589..20308)
                     /gene="phoU"
                     /locus_tag="PKM94_00885"
     CDS             complement(19589..20308)
                     /gene="phoU"
                     /locus_tag="PKM94_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOD65219.1"
                     /translation="MTRHLQREIERIKKKILSLSAMVEERLHLSVKSIAERNSEMALR
                     VCESDWEIDRMEVEVEEDCLKILALHQPVAIDLRFLIAVLKINSDLERIGDLAAGIAE
                     QGIFLAARPRVVLPFDFPRMAEIAKDMLKGAIDSLVSMNPDLAAGVCLRDDEIDGLHR
                     EMYRQVEAGMRAQPDQIETYIHMLAVSRQLERIGDHATNIAEDVIYMVEGIIARHRMD
                     QYNHSEESGGDSGTAGPSGSD"
     gene            complement(20358..21263)
                     /gene="pstB"
                     /locus_tag="PKM94_00890"
     CDS             complement(20358..21263)
                     /gene="pstB"
                     /locus_tag="PKM94_00890"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOD65220.1"
                     /translation="MPEDTPPTADNRPRPTPPALPDGEPARPGMADPLRAPQVRFARP
                     SEPEGETACKITVDHQNFFYGHHQALFDINLQIAEKRVTALIGPSGCGKSTFLRTLNR
                     MNDTIPGTRMEGTVRLGGLDIYRDIADLSTLRTRVGMVFQKSNPFPKSVFDNVAYGLR
                     VNGVTDRQIVAASVEDALRRSFLWDEVKDKLDQSAYMLSGGQQQRLCIARALAVRPEV
                     LLMDEPASALDPISTTKIEDLIGDLRDNYTIVIVTHNMQQAARISDYTAFFYEGRLIE
                     YGPTRRIFTKPSLKRTEDYVTGRFG"
     gene            complement(21256..22221)
                     /gene="pstA"
                     /locus_tag="PKM94_00895"
     CDS             complement(21256..22221)
                     /gene="pstA"
                     /locus_tag="PKM94_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOD65221.1"
                     /translation="MSAVPRGLAARPGPPAPDFQLKAKGTGLLPKSLTLAAVAIIALM
                     LAVILANILIGGREVLSVDFLTHPPEQGMQAGGIFPAIFGTVALVLLMTIAVVPIGVL
                     TAVYLHEYTRPDSKITRLIRIAISNLAGVPSIVFGLFGLGFFIGFVGGGLDAVFHGGA
                     PGPVWGQPAIVWAAATLALLTLPVVIISTEEALRTIPGELKDASYALGATKLQTIRRI
                     ILPQAMPGILTGGILAVSRGAGEVAPIMFTGAAYYLPYLPTRLNDQFMELGYHIYVMA
                     TQSPDIEATKPILYGTVLVLLVLTFVLNFTAIIIRSRLRARRPNA"
     gene            complement(22218..23177)
                     /gene="pstC"
                     /locus_tag="PKM94_00900"
     CDS             complement(22218..23177)
                     /gene="pstC"
                     /locus_tag="PKM94_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOD65222.1"
                     /translation="MQPYQFRPKSKIREFLGERLIQVTALTALVSVALIFIFIFKEAV
                     PIFTDESVREEVTLEKMVVKQEWKAGREASCKWQPESGTPKYSLLPLFVGTLKAALIA
                     MLFAVPLGVGAAIYSAEFASRRLREFIKPVVELLAGIPSVVLGFFALLVLATVVQNAL
                     GLTYRLNAITAGLALGLAVIPIVFTVAEDAMTSVPQSLRDAALALGANRWQVSRTVVL
                     PAALPGIAAGVILGFGRAIGETMIVLMASGNAAVVSLAPGDSVRTLSATIAAELAEVV
                     FGSAHYSVLFLLGTLLFVFTFLINLGGDVALRRIRTRLQGRTS"
     gene            complement(23279..24127)
                     /locus_tag="PKM94_00905"
     CDS             complement(23279..24127)
                     /locus_tag="PKM94_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOD65223.1"
                     /translation="MKKVLIGGALLLVAGVALSAGAITMKGSDTLVRLGQRWAEEYMK
                     QNPGTVIQVSGGGSGTGIAALLNGSTDICQASRDMKEKEYQLAEKNGKEPFRVAVALD
                     GIAVFLNAKNPVGELTVPQLKGIYTGAIVNWKEVGGPDAPIILYGRENNSGTYVFFKE
                     HVLNDEDYADRTQTLPGTAAVVNAVGKDPNGIGYGGLAWATGVKFAAVRQNDTAAAVM
                     PSLETVSDGRYPISRDLYWFFNGPPTGELKKLLNWVLSPAGQTMAADIDYVPLPAERA
                     RANQVP"
     gene            complement(24223..25338)
                     /locus_tag="PKM94_00910"
     CDS             complement(24223..25338)
                     /locus_tag="PKM94_00910"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOD65224.1"
                     /translation="MKRGIIVFLAASVVGAASAAEWTDNVKVKGDLRYRHEVLDRNSD
                     TSPARNRQRLRARLAIEGRVSETAKVVVGLASGSDDPVSTNQTLGESFTTKNLGLDLA
                     SLMYRPARLSFLTLTAGKMEKPFIIPGHSELQWDGDLNPEGAAAALEHNFEKLTLAAV
                     VSGLWIQERSKTKDSWLGSGQVSARLSLNEDKSGLLVGAGYFDYQNIKGFPLFYDEED
                     AFGNSAVEAQFGGETVNVYAHDYNIVEAFVELTHPLGAVPAALFGHYVNNIEADSLNA
                     GWSLGASAGKIKNPGSWTVRYVYRELEADAVVGAFTDSDLGEGGANASGHEVSAACQV
                     AGNTTFSATYFYNTIGIGASDKAEYLNRLQVDLQLKF"
     gene            complement(25535..27322)
                     /locus_tag="PKM94_00915"
     CDS             complement(25535..27322)
                     /locus_tag="PKM94_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65225.1"
                     /translation="MRQRRMLWQLFPSFVLVVALAAGGVYLYTRQAYRTAHYHEAVHD
                     LEMRARLAERVITPYLDAHDNDALDSLVVALGRAGRTRFTVVGADGRVLADSEEDPKH
                     MEQHRFRPEIAAALRGEMMASRRYSSTLQRHMLYTAVPVLADDRVVGVVRTAIPEDAA
                     EGGIGTVTGTLAGDVVVGGLLLVLLAAVISRLVARSVVRPIGDLRAGADRFARGEFDY
                     ALPAAGHQEIAGLAQAMNEMAAQLHERMEAIGRRRNEQEAILAAMVEGVLAVDTSERI
                     LTLNAAAARLLRIRPEEAVGRSVPEVVRNSELHRLVSETLHSGAPVEGTIELAGDDER
                     QLQVHGTTLRDGSGNAAGGVIVLNDITRLRRLENIRTDFVANVSHELRTPVTSIKGFL
                     ETLLDGAMDRPEDARRFLEIIKRQADQLNNIIEDLLALSRLEQERDGEGCEVTRRPVG
                     GVLRSAVQSCESRAREKGIALLCEGDLDLVAPINPSLLEQALVNLLSNAITYGPAGST
                     VRAGARRSVEGVELFVADEGPGIEPRYHERIFERFYRIDKARSRDQGGTGLGLAIVKH
                     IARAHKGRVTVRSAVGQGSTFSIHLPHSA"
     gene            complement(27322..28011)
                     /locus_tag="PKM94_00920"
     CDS             complement(27322..28011)
                     /locus_tag="PKM94_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65226.1"
                     /translation="MAKEPILVIEDEQDIQELIAYNLAREGYRVVRAVTGEDGLACAR
                     REPPALVLLDLMLPGVDGLEVCRQLKHSPATRAIPIVMLTAKGEESDIVAGLEMGADD
                     YIAKPFSPKVLIARIRAVLRRKATGPEDAAARLAIHGFIIDPGRHEVSVDGRPVQLTH
                     TEFQILHLLARRPGWVYTRYQIVNQVRGEDVIVTDRSVDVQIVGLRKKLGAAGRYIET
                     VRGVGYRLREN"
     gene            complement(28150..29514)
                     /locus_tag="PKM94_00925"
     CDS             complement(28150..29514)
                     /locus_tag="PKM94_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65227.1"
                     /translation="MTVRILLADDDASLRRVLQFKLERQGFAVTAAGDGAEALDRLTR
                     ERFDLLLSDIRMPRLSGIELLERVRGVQPDLEVIFISAHAEVAQAVQAVKLGAFDYLT
                     KPFEDEQLFVAIDKALEHRRLAEENRLLRGRLRGRDVMRKLVGVSRPFREMMALVEKI
                     APTDATVLIAGESGTGKEMIARAIHYGSHRADRELVVVNCAAIPRDLIESELFGHVRG
                     AFTGAVRDKRGKFELADRGTLFLDEIAELSIDLQAKLLRAIQEKTIEPVGAEKPVEMD
                     VRLIAATNVDLKERAARGRFREDLYYRLNVIPLRVPSLRERREDIPILVKEFARAFAP
                     SAPLAVDAELMARLAAYAWPGNIRELENLIERMAILRRGEVLTTADLPPDFGRDVGPA
                     EARPAAGDPEPERTLPETEKQLIIKALTRTGGNRSRAALQLGIPRHVLVYRMKKYGLH
                     GPDA"
     gene            complement(29511..30629)
                     /locus_tag="PKM94_00930"
     CDS             complement(29511..30629)
                     /locus_tag="PKM94_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65228.1"
                     /translation="MQKTPAHPESSPLAGPRSGSLTESLPERTRYRLAIIGGIILVTV
                     SIHYGWVLEPIFGPQHWIHAIHGRLCYIPIVLAASWFGLRGGLGAAAAISLLVLPFIF
                     VNDLGAHNTAGEVTEIVFYFALGGLIGGIVDRERRVRRRQQETELALERSQRQALVGR
                     MASTVAHEIKNPIASIKGAVEIICDKDTPEADRAEFREIAVSEIRRIDGTLRDFLEFG
                     RPREPHLAPMNLSETVAAGLRQIEAPAAHAGVRLVRHIQPDVAIVGDREKIHQVMLNL
                     LLNALEASAPGGAVRVAVESEAGGARLTIADGGHGIPPEAVGRIFDPFFTTKSSGSGL
                     GLAVARQIVESHGGSIAVESTVGAGTTFTLRLPAGEVR"
     gene            30850..32163
                     /locus_tag="PKM94_00935"
     CDS             30850..32163
                     /locus_tag="PKM94_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD65229.1"
                     /translation="MKSRVLHAPTSWFIVALLLVLSISGASRGAEPALADTVLPASPA
                     LDDYLRVALNRSPDVRRAYESWRAEAAQTGYAGTLPDPMLMFEYMVDPKQPRLGARQE
                     IPWLGGLRAEKAMAAAAARAAYDRCEAARLAVIYRTTAAYYNYWNLSRQVALMRENLQ
                     LLTFWEEVVRTKYRAALASHPDVIKAQVELAAMDNQVHSLEDRLGPSRQQLLAELNLP
                     AATELPVPAGVVPPAVTIDETTALAAIDSANPDLRAMAAMVEQERAGVRAANAARYPG
                     LTVGADYLFAGRSADPGMTGDGADTWVLSASVSLPLWLGKNQARSRAAEARERAAAYD
                     LTESRNRLRTYAADVMYQYRDARRRVELYAGGLLPKAEEGLNAAYTAYQAGALDFLSL
                     LDAQRQLLEFQLAHSEAVAAAAVRLAELEMLAGGRLTGETPATER"
     gene            32204..34150
                     /locus_tag="PKM94_00940"
     CDS             32204..34150
                     /locus_tag="PKM94_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD65230.1"
                     /translation="MNDFEAAGGRSPRSRIDRFRRTRLGLAVQIALVMGVFALGLMIG
                     GGGSPAPSADSGRPGAEEANAPVLWTCSMHPQIQLPKPGKCPICFMDLIPVETGRGSG
                     RLGPRQIAMSEAARRLARIETAPVRRADAEADIRLVGQIDYDETRLAYIAARVPGRID
                     TLFADYTGVTVRRGAPLVSLYSPDLLSTQQELLQAKKALAAAAQSAGLVRATAESTLA
                     AARERLRLWGLTAEQIERIEAGGQPSDHLTILSPISGTVIEKEALEGEYVETGMRIYT
                     VADLSRLWVILEAYESDLPWLREGQRFTFTAQALPGETFSAVVSFIDPVIDPESRTAR
                     VRAAVDNAAGRLKPKMFVSGEVAARLGSESGLARSTGRPDAPLLIPTSAPLVTGTRAV
                     VYVKTAEDGDGPVYEGRDIVLGPRAGDFYVVANGLAEGEEVVSRGAFKIDAELQIQAK
                     PSMMSPAPLARRAADSASVHGDHPAAGADVAISPDARASLSPLYEAYFEVQMALAKDQ
                     FEASRRGLDDLAAKTAAVDMSLFAGRAHALWMDLSRRLQTAARAGAAAGDIAAVRNAF
                     RDLSATAIDLHRAFGHADDRPFYLTYCPMAFHNTGAYWLQTEGIVWNSYWGAAMLQCG
                     EIRDTLPALSAGAGITAPDSGQGR"
     gene            34154..37939
                     /locus_tag="PKM94_00945"
     CDS             34154..37939
                     /locus_tag="PKM94_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD65231.1"
                     /translation="MSNSRPPDHSARASLLDRLIGSCLDNKLVVILVALAVIAWGVIV
                     APFDWHIPGLARAPIPVDAIPDIGENQQIVFTEWMGRSPQDVEDQITYPLTTTLLGIP
                     GVKTVRSYSMFGFSTIYVIFKEDVEFYWSRSRVLEKLNSLPSGTLPAGVQPMLGPDAT
                     ALGQVFWYTLEGRDPEGRPAGGWDLEELRSIQDFHVRYALQSADGVSEVASVGGFVRE
                     YQIDVSPDAMRAAGVTLEDVFEAVQRSNIDVGAGTIEINKVEYAIRGLGFIENLDDLR
                     ATVVKSVRGVPVRIADVATVQLGPAPRRGALDKGGAEAVGGVVVVRFGENPLQAIKNV
                     KAKIAEIEPGLPKKTLPDGTTSQVKIIPFYDRTGLIYETLDTLNMALVDEILVTIIVI
                     LFFVMHLRSSLLISGMLPLAVLMTFIGMKLFGVDANIVALSGIAIAIGEIVDMGVVLC
                     ENILRHLDEAPPEANRRDVIYRASTEVGGAVLTAIATTVVSFLPVFTLEAAEGKLFQP
                     LAYTKTFALVASIMIALTVLPPLAHLLFTRGRRSGVSRRLLPLGLAAAAAAAFVWQLW
                     LTGLGLLGLAAFYRLRHRLPARAQAAAPRYANYLLLAVVGWITALHWEPLGVEAGLFL
                     NFLFVAVLLGGILLLFHLLIRAYEPILRWCLAHKKMFLAFPAALVLTGLVIWIGFDAV
                     FGFVPQFVERTSPYVALRHMFPGLGQEFMPDLDEGSFLYMPTTMPHASIGEALDVLQT
                     QDMAFSAIPEIESVVGKLGRAESPLDPAPISMIETVITYKTEYLLDARGRRMKFRYDE
                     EKDEFVRDQRGQLIPDDGGRPFRQWRDAIHSPDDIWKEIQEAGKLIGTTSAPKLQPIA
                     ARVVMLQSGMRAPMGVKIKGPDMETIERVGLEVERILKEVPSIDPATVVADRIVGQPY
                     LEIAIDRAAIARCGLQIRDVQDVIEVAIGGLPLTTTVEGRARYAVRVRYQRELRGSLE
                     SLARILVPTPAGAQIPLGELAEIAYVRGPMMIKSEDTFLVGYVIFDKRPGRAEVEVVD
                     EARRTLAARAAAGEFDIPAGVSYAFAGSYENQVRAEHKLRIVLPLTLFIIFMILYFQF
                     RRLPATFLVFSGIFVAWAGGFLMLWLYGQDWFLNVSLFGANLREVFQVHPYNLSVAVW
                     VGFLALFGIATDDGVIISTYISQVLDRTRPTTAAEVREAVVAAGKRRIRPALMTVATT
                     ILALLPVLTSSGKGSDIMVPMAIPSFGGMTVVIITVFVVPVLYSAIDEARAARARRT"
     gene            38106..39074
                     /locus_tag="PKM94_00950"
     CDS             38106..39074
                     /locus_tag="PKM94_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOD65232.1"
                     /translation="MPDSAPVERPIKVGISTCLLGQKVRFDAGHKHDRYITDVLGGYF
                     RFVPVCPELEVGMGVPRESVRLEGAPDAPRLVGTRSGADWTERMNRYARERTARPDLA
                     DISGYILKKDSPSCGLERVKVYPPSGMAERKGTGLFAAALRQRFPLLPIEEEGRLNDH
                     RLRENFIVRVFAYDRLQTLFRERFSRGRLVAFHTAHKYLLMAHSPKHYAELGRLVAAV
                     SRHSPAAMREQYRRLFMEALAVPTTTRKNVNVLQHLLGYLKHRLGPEEKQDILNVIAD
                     YHRELIPLVVPLTLLRHYVDKHRIAYIQDQVYLNPHPKELMLRNHV"
     gene            39599..40270
                     /locus_tag="PKM94_00955"
     CDS             39599..40270
                     /locus_tag="PKM94_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65233.1"
                     /translation="MIKRLTLLTLVALLVAPAFSNAKDMTGKYGLGYFTTDAPIGFRY
                     WATPKLGIDVGVGFKSDAAWVGEYDETEDRTVYSKETASSFWFEVGFPYVVVATDQVN
                     FWVRPGFQFASLDDRIFSPDSKVYDADDDFEEQGMDDVGLDAKFTQMTISLTPAAEWF
                     ISDNFSIEAGHGIKIQMLKFPDDKIPGSDPAEYYYGDAGGETETSIVTFSADNVFFAF
                     HFYFK"
     gene            40492..41142
                     /locus_tag="PKM94_00960"
     CDS             40492..41142
                     /locus_tag="PKM94_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD65234.1"
                     /translation="MFKRAAMFTLALLLIVSMPTWAQNQIKPVVYAGGGIDLLLSPDL
                     LKDYWKMGYGFGGGVGIQVKPNLELIGRVSFNTFPFDADKFLDDMDAPAGVSVDGLDF
                     RSLEFGADMKYIFMSDQANMMFKPFVVAGLGFANIKATDATITAEDEDDIEEVHLPFG
                     DASETDFSFNFGGGFDYMFAPKAGAWVDVRYTYIASEGDAISYLPIRAGVKFLFGN"
     gene            41977..42576
                     /locus_tag="PKM94_00965"
     CDS             41977..42576
                     /locus_tag="PKM94_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD65235.1"
                     /translation="MKATFVAACVCLLILGAALSAQAQSEKPTLGLGYHGILAGDVLQ
                     GLSGRIWTSPKVGLEANLYYAHVGVDVKMEGGEGQEDDEYDISGTLTDFSGKFLYAVA
                     NHSQSKFYVGAELGYGHVSVEVETPGLPAALAEGFDVITLGPLMGAEFSFAGIPEMSV
                     DWELGYRTNLVDIEVADYDISLSMGVLAASVGVHYYFGR"
     gene            complement(42661..43083)
                     /locus_tag="PKM94_00970"
     CDS             complement(42661..43083)
                     /locus_tag="PKM94_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65236.1"
                     /translation="MSEDRRRILNMLAEGKITADEAERLLEALGRTAERTAPETTGRT
                     AGARPKYLRVIVQPKQGQRDQVNIRIPLALLRAGVKLGGVVPDSVKGRINEALAEKGF
                     TVDLGKLNMEDLERLIDALGEMSIDVDDERESVRVCCE"
     gene            complement(43076..43459)
                     /locus_tag="PKM94_00975"
     CDS             complement(43076..43459)
                     /locus_tag="PKM94_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOD65237.1"
                     /translation="MAHEWQELTRLTGDKEVIVERVRLVVEGIALEGEFELPPLAKLT
                     AEDQLFVAAFVRSHGSIKKMEEVFGISYPTVKNRLNRIGAALSFAQVQVEPGDRREEL
                     LDRLERGEITVQDALSQLEKEQNHE"
     gene            complement(43742..44524)
                     /locus_tag="PKM94_00980"
     CDS             complement(43742..44524)
                     /locus_tag="PKM94_00980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOD65238.1"
                     /translation="MRTAFIFCAALLWATAAGAAVHTETVEYRHGKVVLEGYLAYDSS
                     LIGTGPVPGVLVVHEWTGLGDYVKIRARRLAELGYVAFAADVYGKGIRAKNADEAAKL
                     AQTYYQDRRLFRDRVQAALAVLRGQELSDDRRLAAIGYCFGGSAVLELARAGADVRGV
                     VSFHGILSTTQPAEPGTVKAKILVLGGADDPYVPDSQVAAFEEEMRRVGADWQLTEYG
                     GAVHSFTNPDAGNDASRGAAYNEAADRRSWEQMKMFFEEIFR"
     gene            44783..47086
                     /locus_tag="PKM94_00985"
     CDS             44783..47086
                     /locus_tag="PKM94_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOD65239.1"
                     /translation="MNYFRLWLAMGVVIVASFAVLGYFGGDIYRQAPPVPDRVVTSDG
                     AVLFTGSDIKDGQNVWQSTGGQQVGTVWGHGSYVAPDWSADWLHRESEWILNEWARTE
                     YGVSAYTSLSGPDQAALRERLREEIRPNTFDPKTGDLVVSPVRARAIEAVGRHYAGLF
                     GGDPALGKLREAYAIPGNAVKDPARLAKLNAFFFWASWACSTNRPGESITYTQNWPPE
                     DLIGNRPTSSSIVWSVISFVLLLAGIGALAWYYAVLKHKPVGEVALPEQDPLLALQPT
                     PSMKATLKYFWVVIGLMIVQIIMGILTAHYGVEGSGFYGFPLAEWLPYAVTRTWHTQL
                     GIFWIATAWLATGLFIAPAVSGYEPKFQRAGVNFLFVCLLIIVVGSMFGTWYGTRQQM
                     GLTANFWFGHQGYEYVDLGRFWQIFLFVGLFVWLGLMGRGLWPALRKPGPNRHLLVLF
                     FIASAAIALFYGAGLMWGRQTNLAIAEYWRWWVVHLWVEGFFEVFATVVIAFLFTRMG
                     LLRTATATAGVLFATTIFLAGGIIGTFHHLYFTGTPTAVLALGATFSALEVVPLVLIG
                     FEAYENLSLSRAKPWLQAYKWPIYAFVAVAFWNLVGAGLFGFFINPPIALYYMQGLNT
                     TPVHGHTALFGVYGMLGVGLTLFCLKGLASRYAWKTTWLAVGFWCINIGLALMVLLSD
                     LPRGLMQTWASVSHGMWFARSAEFMQTGLMDTFRWLRVIGDTIFAVGVFALGYFVFGL
                     KFGWSVSGQLDRPVGAAPERPEEVTRP"
     gene            47153..48487
                     /gene="ric"
                     /locus_tag="PKM94_00990"
     CDS             47153..48487
                     /gene="ric"
                     /locus_tag="PKM94_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOD65240.1"
                     /translation="MLRYKSGFSISLAVMLLVAASAAAHCDREDGPVANDARAALAGG
                     EFTKIAVWVGEAQTDELRRAFDQTRAVRSQSEEARELADRWFIETAVRLHRAAEGMPY
                     TGLQPAGLPAPAEVAAADKAYAADDVEPVIELLESSLEEHVRSLFTEAMRAKKEGDKS
                     LEAGREKIDAYVRFILYVHGLVEKIEAGPEHGVGEHSSVPDPHNTKGATMNTVSADRT
                     VGDLVTEQPGRAKVFEDLGIDYCCGGHRPLTEACAEKGIDPENVLAQLARVDAARRPD
                     ADADYGSMSLTALADHIVDTHHAYLRQALPRANQLAAKVAQAHAASPKGADLKRVDEH
                     VRALAAELQSHMVKEEHILFPMIRRIDAGERGLVIDGPVMVMMREHDEAGEALAAIRT
                     LTDDFALPAWGCNTYRAMLDALQELELDLHQHIHKENNVLFPRAQQAAAVGA"
     gene            48640..50148
                     /locus_tag="PKM94_00995"
     CDS             48640..50148
                     /locus_tag="PKM94_00995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD65241.1"
                     /translation="MNRPLVVLLALACAVAPAVASADPAAPTALPDTPAGRCLAEFVS
                     ALNSSDSAQWIAFLAQHPPADANDSAAIQERRFGLMRTVHGDFGGLDPVKVLESSEYF
                     LSVILRGRVPVQASGYLRLAFTVTDQPPHRWVRMEAEMLDEDPFAPPPPRMESDEQLA
                     AWVDSVVTAMAAAGQFSGAILLAKDGVPFYSRAVGPADRCCGAPNLLDTKFNLGSMNK
                     MFTGVAIARLAQEGKLAFEDPLIRYLPDYPDREAAAKVTIHQLLTHTAGVDDYWEELF
                     DTSFWEIKSVDGYYQLIKDKPLRFEPGTQYRYSNGGPILLGVIIERITGQSYYDYVRD
                     HIFRPAGMINTDCYEVDVPTPNLAIGYTRLDYSGRRDTVWRANYFMHAVKGGPAGGGY
                     STVEDLLAFDRALRSYRLLNQAFTDTVLAGRVAMEPGPVSTRKADEMYAYLFGDMRTN
                     GHRIVGHSGGAPGINAVLEMYWDDGYTVAVMANIDEAASTLAARIRRVLTQP"
     gene            50280..51659
                     /locus_tag="PKM94_01000"
     CDS             50280..51659
                     /locus_tag="PKM94_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOD65242.1"
                     /translation="MDDVTLLSRVQFGLTVGFHYIFPVLSLGLGLFLVIAEGIYLKSR
                     DPLWESITRFWVRIFGLIFALGVATGIVMEFQFGTNWSVYSRFVGDVFGSALAAEGIF
                     AFFLESGFLAILLFGWDRVKPRTHFIATLLVALGAHFSAVWIVVANSWQQTPAGHHLV
                     SHAQGMRAEVVDFWQVIFNPSFVDRITHVFMGAWQAGAFLVLSVSAWYLLRRRHRAAA
                     VGSIKIAVVIAAVASLAQLVSGHSSANVVARHQPAKLAAFEGHYPESAPGDSYLFGWV
                     DEAGEIVYGPKIPGLLSFLAYSDFDAPVTGLRAFAPEDRPPVNLTFQSFHLMVAIGLA
                     LIAISLAAAWYWRRGTLWEKRWFLWILVLAVVLPELANQLGWFSAEVGRQPWIVYGLL
                     RTSAAASVGIGGAHVWGSLVMFAVIYILLGALFIYLLNDKIQHGPDGAEPPAPSGSEP
                     VIAKEGPRA"
     gene            51659..52678
                     /gene="cydB"
                     /locus_tag="PKM94_01005"
     CDS             51659..52678
                     /gene="cydB"
                     /locus_tag="PKM94_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOD65243.1"
                     /translation="MTDLNSLWFALVGLLFIGYAILDGFDLGVGALHLFTHTDEERRV
                     MINAIGPVWDGNEVWLVTGGGALFAAFPEVYATAFSGFYVPFMLLLFALILRAVAIEF
                     RSKRASHAWRRTWDVSFTAASLLGSLLIGVALGNIAWGIPLGPGYEYLGSFWDLLNPY
                     ALLVGVTTVALFMMHGAIYLTLKTEGAIHDKVRGWINNTIIFFIICYATTTMATLLFV
                     PHMTDNIRNDPVLFVLPLLNMLAIANIPREIHHGHDFRAFLSSAAAMFFLLVLFGVDM
                     YPRLILSSPLTEHSLTIWNAASSDRTLGIMGIIALIGLPLVLTYTITIYWVFRGKVRL
                     DRSSY"
     gene            52777..53556
                     /locus_tag="PKM94_01010"
     CDS             52777..53556
                     /locus_tag="PKM94_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65244.1"
                     /translation="MNAKSLVMVIALWAAAAAASAQELLPPDTTVTVADIMNRMAALM
                     GGPESVGGIRTAHIVGHSRLPNSDRAATIEVWWAQPNLFRAVTRPPVGDAAECGFDGR
                     VCWQREPKTGPRIISGEEAGDLILEYHPGRFILWRDLYLNADLIGADSVFGELCDIVR
                     LVPDQGRAQDVYLSRSAGLPVMIKTERAMGARLVAVNIYLSDYRATGGTVLPWRSLME
                     FSGRTRTMQVDKVSLNGDPPADIFSLPDDVRVLAAAPDDPR"
     gene            complement(53647..54444)
                     /locus_tag="PKM94_01015"
     CDS             complement(53647..54444)
                     /locus_tag="PKM94_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65245.1"
                     /translation="MKQLGFTVVLAGVLLWAGLGLAAAQEPVPPAQAAAPAGITPEAA
                     ALMDRMVEATGGLETLAKINNRYSRSTLEIAGMGLSLSIETYSAKPNLQFAVATSEAT
                     GSIESGCDGVTAWERSVMTGPRVLEGAEAAERLRDAHFDKYAVWRQIYQSAELAGVDT
                     VNGEVCDVVALTGEGSQPEKLSISRDSGLPLKMVMTVEHQMGQIPITVYMTDYRNVDG
                     MKMPFLTTMDVMGQTRAIKVDSVAHNVELKPGLFDLPEDIKALMTKE"
     gene            complement(54472..55779)
                     /locus_tag="PKM94_01020"
     CDS             complement(54472..55779)
                     /locus_tag="PKM94_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD65246.1"
                     /translation="MKRVRAFCVLAALAFCLPAAAADGGDQLTPEQRRLCEQSFDQVW
                     QIVYDRHYDTTFGGVDWPGLRIELRPKLDTCETMADVRVLLAEMIHRLGLSHFEILPG
                     EILSTMDIPDNPVDQAGVTGMVVRVVGGAALVARVRPETPAAEAGIRPGWEVVTAGGD
                     KVTEVLAPLQEQYADHIRYPIYAATAVLNRLAGKVGDTVTATFRDSSGNLVTDSLVLA
                     ERVGQRTVFGNLPPFYLTIDTATLEGGIGYFAFNCFFDPGMLMGRFQEFVAAHRDAPG
                     WIIDVRGNPGGIGGIAMGLAGFMVSEKNLLIGTLTTRESNLKMVINPRAETYDGRVAV
                     LIDGLSGSSAEFFSGGLQAIGRARVFGSRSMGAALSAMTEKLANGDALLYVFADFLSA
                     DGTRLEGTGVTPDEPVELTRESLLAGRDRVIEAAADWIRQQEK"
     gene            complement(55879..57123)
                     /locus_tag="PKM94_01025"
     CDS             complement(55879..57123)
                     /locus_tag="PKM94_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOD65247.1"
                     /translation="MKRMRVLWPAVIGLVCLALACSKNATDDGDNGGEDDLTAPAAIT
                     DLRALTVTTGSVVLGWTAPGDDGLTGIANAYDLRASADTITDATFVWAVPLADIVSPG
                     VAGQPQTVVVDDLLSDSIYYFAMKTIDEAGNASGLSNCCRVHIPAELVVAFEDTAFER
                     VVREHIHKPTGDIHSSDVDTIGELNANEQHIADITGVEYFTSLAFLHLLGNNITDLSP
                     VAGLPALVSLNAVGNDISDVSPLAGLTGLRDLALSQNPLATLAPLAGLGNLTQLSLDY
                     VSVADLSPLYGLEKLELLRLGGNNLSDIGFLSGRFPQLRDLTLANNSITNLAPLAGHA
                     ALQDIYLPGNNIVDLSPLRNLANLRIIHLNYNAITDLQPLVDNGGVGQGDAVYLKFNP
                     IPDPSTNGQVQALRDRGVEVDI"
     gene            complement(57270..58103)
                     /locus_tag="PKM94_01030"
     CDS             complement(57270..58103)
                     /locus_tag="PKM94_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD65248.1"
                     /translation="MTLTTDRRMFLAACATALLLPETLGALVPQDSASADSTAGALDN
                     DRDLQKILRELPQAREHLPRLRAACARHAGIYPIPPVLAAKVLAIESGFADDAISSSF
                     AAGAAQLMHTTARELGAALPEADVFADQDEVLRLRGAFQRKMSEAVAAFQEGDDAGAR
                     AQRAEAETLREDYDLLHATTMESFRRRMFALTPEARRAYDARFDPAVADDLVVRYLAM
                     LARGVKRDLNLSDEAHVLLLAGVAYNAGPGSVKRATGIPVVAQNVEYANKLMAFQRLS
                     L"
     gene            58283..58840
                     /locus_tag="PKM94_01035"
     CDS             58283..58840
                     /locus_tag="PKM94_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65249.1"
                     /translation="MGAGKTVARSRLSEDLVRVFKSLVIWSFGAMLLAPFVSPRAARP
                     EPVPSDTAARLEPRSVTSGLFLAAVFPGPFIHGAGNYYAGAPAAGAVMTVAEIVGAGA
                     IIAGFASGGLDLFDNEEDDETGEALMAGGLVLFWGSWLADIATTPFQVHRYNRRLAQR
                     GMVFGVYAAPPAQGRPPAFGVTIRF"
     gene            complement(58887..59075)
                     /locus_tag="PKM94_01040"
     CDS             complement(58887..59075)
                     /locus_tag="PKM94_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65250.1"
                     /translation="MATFLLWLILLAVAWPLALLALVLYPIVWLLLLPFRVVGAAVDG
                     VLELLRAVLTLPARILGR"
     gene            complement(59131..59610)
                     /locus_tag="PKM94_01045"
     CDS             complement(59131..59610)
                     /locus_tag="PKM94_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOD65251.1"
                     /translation="MAAAPRILVIGLGNDFRSDDRVGLYVARRVEAAGRPGVGVMIGV
                     ADALDLLDLWNRADFVILVDCAVSGREAGTVHRFDALREPIPEDLFCRYSTHAFSVVD
                     ALALAAAMGQMPPQLIVFGIEADTCAPGTEMSLPVEQAAEHVIGRIWTEIDAFVETR"
     gene            complement(59617..60906)
                     /locus_tag="PKM94_01050"
     CDS             complement(59617..60906)
                     /locus_tag="PKM94_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOD65252.1"
                     /translation="MKNRTITVDMLARVEGEGGLTVRVKQDKVTEVRFNIFEPPRFFE
                     AFLRNRRFSEAPDITARICGICPVAYQMSSAQAMEAICGAAVEGPLRDLRRLLFLGEW
                     IESHALHVYLLHAPDFLGYASGIHMAADYPDIVKRGLRLKKIGNDVMVLLGGREIHPI
                     NIRTGGFYSVPSRAAVLALKKDLEWALEASLETVKWTAGLSFPDFEPDYEMVAIRHPE
                     EYAITEGRIVSNRGIDIPFNDFFKYFEEEHVKHSTSLHARVIERGHYLVGPLARYSLN
                     YGQLPEIAQRAAREAGLGPTCRNPFKSIIVRSVELVFACWEALRLIERYQPPAQPAIP
                     VPPRAGTAAWATEAPRGMLLHQYTLNGHGLILSARIVPPTSQNQKAVEDDLLHFVERN
                     LHLSQEDLTWQCEQVVRNYDPCISCSCHFLKLRIIRE"
     gene            complement(60896..61675)
                     /locus_tag="PKM94_01055"
     CDS             complement(60896..61675)
                     /locus_tag="PKM94_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOD65253.1"
                     /translation="MAKSVKPKLAVWKFASCDGCQLSLLELEDELLAITGAVEIANFL
                     EASREVVPGPYDVSLVEGSITTPHDAERIHKVRRASKLLIAIGACATSGGIQALRNFA
                     EIRTYIPAVYARPEYIETLRKSTAIADHVHVDFELRGCPISKYQLLEVVNALLNGRKP
                     NVPPYSVCLECKRRGTVCVMVSQGLACLGPVTQAGCDALCPSYNRGCFGCFGPKETPN
                     PSSLANWFKEQQMSSDDIVRTFRQYNAYAEPFRKESEAHEK"
     gene            complement(61680..62549)
                     /locus_tag="PKM94_01060"
     CDS             complement(61680..62549)
                     /locus_tag="PKM94_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOD65254.1"
                     /translation="MAITKTNAAAESPALLDPMLGQPYRIGRVVKETYDTFTLELAPT
                     EEGNGFAFLPGQFNMLYVYGVGEVPISISGDPAKPRALIHTTRAVGSVTKQMQKLRKG
                     DVIGVRGPYGTAWPVEEAKGRDVVVVAGGIGLAPLRPTLYHLLKHRKRYGKVILLYGA
                     RTPDDVLYPKMLAQWGARPEIEVLTTVDRGMGQWTGNVGVVTTLVPKVTFDAANCLAM
                     ACGPEVMMRFTALALSKRGLADEQIYVSMERNMKCGIGLCGHCQTGSLFVCKDGPVFR
                     LDRVKPLFTQREI"
     gene            complement(62536..62994)
                     /locus_tag="PKM94_01065"
     CDS             complement(62536..62994)
                     /locus_tag="PKM94_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD65255.1"
                     /translation="METLEKILSEHSFFKDLSPAHNAIICGCAANTVFPKGEFLFREG
                     EAADKFYVIRQGKVLVETHVPHRGPVAIQSREAGEVLGWSWLVPPYRWHFDARAVETT
                     RAIVFDGKCLRERFASDHELGYEVMRRFVTVIAERLDATRMQLLDVYGHH"
     gene            complement(63009..64160)
                     /locus_tag="PKM94_01070"
     CDS             complement(63009..64160)
                     /locus_tag="PKM94_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD65256.1"
                     /translation="MSTQTHHTYQPRVIERSHLQALFDALARRGHRLLGPTVRNGGIV
                     YDDIQRVEDLPLGWTDHQEGGTYRLTHTGTAAVFGYVVGQGSWKKYLHLPTATLWEAE
                     RSAEGFRTAPAAEPPPRRAFIGVRPCELAAIAIQDKVFTAGPYADPLYRRQRENLFLV
                     AVNCTRPGGTCFCGSMGTGPEARSGYDIVLTELSANGRHVFIAEAGTVEGEAVLAELA
                     APPAGESDRQEAARATADAAHHMGRQLDTDGLKARMEKAVEHPHWAEIAARCLTCGNC
                     TMVCPTCFCTTVEDWTALGGEKAQRVRKWDSCFTVDFSYIFGGSIRPSPYSRYRQWMT
                     HKLASWVDQFGTFGCVGCGRCITWCPVGIDITEEARVFAHSLQRAEAHT"
     gene            64415..66076
                     /locus_tag="PKM94_01075"
     CDS             64415..66076
                     /locus_tag="PKM94_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65257.1"
                     /translation="MAKAPGLRWRITAAVLLAALLPLALAGFGAWIVFGSLFEQRSLE
                     SLRGLVRAHARTIDADLSEHIRLLQVLAASQTRTRLADDAQLDTLLAELNRAGDNSFT
                     DLGVIDAEGDHLAYAGPFDLKGRNYRGDEWFGEAMERGVSLSDVFLGFRGAPHCVIAV
                     RAGNAAEGFWILRGTINSARFDTLVQAGKCNGGDAYIVSRRGLYQTASRRGALLDTAR
                     GIPLVPHEGAADYRATLAGTDLILLTAWLQSGRWLLVVEQDRTALRAPVNQAITRGAQ
                     VVLIAVLILIVTTVIATRHLCRQIDRANAQREELSRAFVRSARLASIGELATGLAHEI
                     NNPLAVISAEHTNLADLLRASDLPEPTRGDLLESVERCRRQVQRCAGITRKMLQFGRR
                     REAAPEPTDITPRLQEIIALLRRRAGVRNVALHLETAAGLRPVLVDPIEFEQVIVNLI
                     NNAVDALPGGGDVWVRAGAAGDRLRIEVADNGVGIPADTLERIFEPFYTTKPVGQGTG
                     LGLSVCYGLVGEWGGRITAESAVGRGTTMRVELPLPHPDNSTARP"
     gene            66087..66932
                     /locus_tag="PKM94_01080"
     CDS             66087..66932
                     /locus_tag="PKM94_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65258.1"
                     /translation="MHNPYQMIRVLLVDDERDFLASTAQALTRRGFRVTTADSGTAAL
                     RLLEGDFFDAVVLDVKMPDVDGLEVFRQVHGLRPQLPVILLTGYASAAQAAAAAESGV
                     AVYLAKPCEIGDLVEHIRNALGARRVVDENGFESAAMEEVSVLLVDDEDELIAALSPV
                     LRRRGMTVSAASGGEEALRVLRESFVDVVVLDLKMPGMDGLAALRHMKREFPDVEIVI
                     MTGHPSADSAVQTIKAGASEYLMKPPDTDELVGAIRKVFRRRRQRLEQKQQRLIDEVR
                     KRYPE"
     gene            complement(67212..68480)
                     /gene="ftsZ"
                     /locus_tag="PKM94_01085"
     CDS             complement(67212..68480)
                     /gene="ftsZ"
                     /locus_tag="PKM94_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD65259.1"
                     /translation="MAEGKHSFNFAEDFIGYANIKVVGVGGAGGNAINRMIEARLQGV
                     EFIAINTDAQVLDANRADKKVQIGIELTGGLGAGANPDVGKRAIDESRAQVAEAIGRP
                     NLVFITAGMGGGTGTGAAPVVAELAKEAGALTVAIVTKPFRFEGAHRINRAETGLEEL
                     RSKVDTLITIPNERLLQIVDRKTTLADAFKVADEVLHQATKGISDLITIPGLINCDFA
                     DVRTVMLERGDALMGTGWGKGEEKAVDAAQEAISSPLLENVSISGARGVLINVTGGED
                     MTLFDVNNATSLIYEAAGNNANIIFGAVIDPTMADEMRVTVIATGFGGVETKRAQVEK
                     DERERAAREVPSGAPISLFPESAVARPPEPAPAAEPEEVLAAAGVPEYGDRGGNGNGN
                     GNGKGKPRVPMFADTDRTVPAYLRRLTDNR"
     gene            complement(68505..69731)
                     /gene="ftsA"
                     /locus_tag="PKM94_01090"
     CDS             complement(68505..69731)
                     /gene="ftsA"
                     /locus_tag="PKM94_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD65260.1"
                     /translation="MATENIIAALDIGTTKVVCLVAEMDTDGRIYVIGHGQAPAEGLR
                     RGVLVDMERTVRSVRKAVDDAALVSGTVIDRVTVGIAGEHIRSINSHGVVTVTRADNE
                     ITAADVRKAVDAARGIAIPVDREIIHVIPQEYSVDDQTGIKDPIGMSGVRLEVEAHIV
                     TASVTTARNIYRALERCGLDADHIVLESLALSEAILTDDEAEHGVVVVDLGGELTNIS
                     IFHDGAIRHTAVVPLGGRNVTNDIAIGLRTTVEQAESLKIAHGAALASQVDPEEMIPV
                     ESAAGHEAKEISRNVLASIIEPRMEEILALVIRELRRADVDDMLAGGVVLTGGGSLLP
                     GTIALAEQLLDIPVRPGQIRGFTSIPDEMNNACFATAHGLLLYGFHNEPELAGTARGK
                     VRGLLKKFEKWISNKF"
     gene            complement(69738..70535)
                     /locus_tag="PKM94_01095"
     CDS             complement(69738..70535)
                     /locus_tag="PKM94_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65261.1"
                     /translation="MGISGRKYRLFALAGLLVGLGLTVTVNFTDACRLRAVTVDGKPV
                     ENWRQQFAMLRPGSLAAQPVERLAKKLLADEDVYKVDVRFELPGGIDVRTNEFRPVCF
                     VVGMETGKLFGLNRRARLIALDAPDINWEQPVFTGLTTGALHSICADPRVLPVIDQLE
                     DLRESRADLYRLLDEIDFGEREYLEVTFAGLPYRLRVRPDHVLEDFDRFVEFTGRYDA
                     DLSGVIRMDLRYENMIICALSDEEMERRAEAARERAKAEAATQEAKD"
     gene            complement(70526..71905)
                     /gene="murC"
                     /locus_tag="PKM94_01100"
     CDS             complement(70526..71905)
                     /gene="murC"
                     /locus_tag="PKM94_01100"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOD65262.1"
                     /translation="MFGKIRKLYFVGIGGSGMSGIAEILHNLGFEISGSDQTASDLTR
                     YLAERGLRIHEGHDAAHLGDAEVVVISSAVGEANPEVVEARRRGIPVIKRAEMLGELM
                     RLKYSIGVAGTHGKTTTTSMIGRILQSGGLKPTIIVGGIVAELGTGASLGEGEYLVAE
                     ADEFDRSFLAMFPTMAVVTNLEADHLDCYRGLDDLGHAFLTYMNRVPFFGSVVLSADN
                     AQLMQMRDRLARPYVTFGFSVEADYRAVEVRLEPGRSVFSLYRRGQYLGDILLRVPGR
                     HNVANALAAAAACTELGVPFELIADGLRGFTGVGRRFEIVGEAGGVTVVDDYAHHPTE
                     VEATLRTARDVYGRRVIVVFQPHLFSRTRDFADEFAQALAIADQCLLVDIYPAREKPM
                     PGVTADLIREKAAARGLGQFTCIGERQNAAREVVQRAQPGDIVITMGAGSITLERKNI
                     LEALRERWG"
     gene            complement(71962..73107)
                     /gene="murG"
                     /locus_tag="PKM94_01105"
     CDS             complement(71962..73107)
                     /gene="murG"
                     /locus_tag="PKM94_01105"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOD65263.1"
                     /translation="MTGRTQEYRLVFAGGGTGGHLFPAIAIADRVRELLAGTAPVEIL
                     FVGTRRGLEYRMRDALGYPLHVINVRGLVRALSPANLLVPFVLVTALVRAWLLLGRFR
                     PDAVVGTGGYVALPVLKAARWRGVPTVLQEQNSFPGITTRQGAKHARKIYLGFDGARQ
                     YLRTEAEVLTTGNPVRRGLSGGDRAAAAAAFGLDPAKKTILVLGGSQGARAINEAVLR
                     SLAGGAPADGCQILWQTGKRDYTEVSAKAGGTAARCALFPFAHDMAAVYAAADLAVAR
                     AGALTLAELAACAIPAILIPYPHAAGDHQRKNARDLAAAGLARVIDENDLPRHDLIAE
                     AADLLEAAEYRTMREKLESARRQGRPATDIIAEDIIDLINRERKARA"
     gene            complement(73104..74228)
                     /gene="ftsW"
                     /locus_tag="PKM94_01110"
     CDS             complement(73104..74228)
                     /gene="ftsW"
                     /locus_tag="PKM94_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOD65264.1"
                     /translation="MVRKTTTRLLLKGGYDEKLLIAYLVAVVAGMVLVYSTSSMLAES
                     RFGSHLHFIGRQFVWAIISLAAIWLVCRIDLQRWAVWSWLLFVLTLGLLALVFLMPAR
                     NGSHRWLFLGPLTLQPAELFKFVMIYFLAFSLANRNRDVTKLKQVWMPYGPMLGAGLV
                     LIVLEPDLGMSMVIFATAVGLFFLAGAKIKHIAYAALPAMGGAALLVFVIGYKKARVM
                     DFLASVMDPLAGSYQSKQAALTLGAGGIFGVGLGDGRQKHFFLPYPHTDFIFAAAGEE
                     IGLVGLLLLLSLLGFILYRGFKIAADQPDRFGFLLASGVVLSLFINIAVNVAVVTSLM
                     PVTGLPLPFISYGGSSLLITSITIGVLLNLSRRMVGRAKQ"
     gene            complement(74194..75585)
                     /gene="murD"
                     /locus_tag="PKM94_01115"
     CDS             complement(74194..75585)
                     /gene="murD"
                     /locus_tag="PKM94_01115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOD65265.1"
                     /translation="MTREERIRGRKVGVIGMARSGLAAALLAKEMGGIPFVTDSGRRE
                     ALAPACAQLEKSGVPFETDGHSDRILQSDYAVISPGIPLSAEIARRIRERGIPLFSEI
                     EFASWFCRGRIVAITGSNGKTTTTTLAGEMFAAAGRPTCICGNIGRPFAEAASSVPPD
                     GVAVVEVSNFQLETIADFRPAVAAILNITADHLDRHGTMEEYKRTKYRITENQGPDDV
                     LVTNLDDPVTSGERIATRAARVYFTTGDATEAGAFVRAGVLWRRKDRAEEPVIPARDI
                     LIPGPHNLQNAAAATVMATVFGVEAAVLAGVLRTFPGVEHRLERVPSVAGVHFINDSK
                     ATNVDSVCFALQSVDTPVYLILGGRDKGAPYEPLAPLARGKVRGVVAIGEAREKIFRA
                     LGREFPVQFADTLEEAVQKCFAQARPGETVLLSPACASFDMFDNYEHRGRAFKEAVAQ
                     LKNGKKNHNPVAP"
     gene            complement(75590..76678)
                     /gene="mraY"
                     /locus_tag="PKM94_01120"
     CDS             complement(75590..76678)
                     /gene="mraY"
                     /locus_tag="PKM94_01120"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD65266.1"
                     /translation="MLYHLLYPLAHQVSGLNLFRYITFRTAGAMATAIFICLILGPFF
                     IGLLRKHQVKERIRAEGPASHQKKAGTPTMGGLIIVSGIIIPTLLWSDLTNFYVLMAL
                     LVTAWLGLLGYLDDYSKALKHQRNGMVARKKFMGQVGLGLVFGGLLLWVAPNGLYDGM
                     TGIPFFKNYMLDLGVLYIPWMILVLTGASNAVNLTDGADGLAIGLSGLAFLAFGGIAY
                     LTGRLDFSSYLQIEYLPGAGELTVFCGAAIGSALGFLWFNSHPAEVFMGDTGSLALGG
                     ALGAIAIMLKKELLLVIVGGVFVVEVLSVIIQVLSYRYRGGKRVFKMAPIHHHFELLG
                     WPEEKVVVRFWIIGALCTLATLATLKVR"
     gene            complement(76678..78066)
                     /gene="murF"
                     /locus_tag="PKM94_01125"
     CDS             complement(76678..78066)
                     /gene="murF"
                     /locus_tag="PKM94_01125"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD65267.1"
                     /translation="MRFDQLAAITGGRVHRAADRTASFRGVSIDTRTLAAGELYVAIR
                     GEKHDGHEFVAQALERGAAGILAEFDHPELARLAGDTPVITVPHTHEAMIALARHYRH
                     TVAARFVGITGSNGKTTTKELTYRLLAAVEPRAYRSPGNLNNLYGVPLAIFRIPADCR
                     AAVMEMGISTDVEMPRLAEIVQPDAVAITNVSATHLEFLGTVEAVAQAKLELVRGARP
                     GAPAVINADDPVLAAEAKKVRSDYVTFALDRAADFRPDAWGIDDAGMMTVVLEGRTFR
                     MPLVGKHQVYNLTCAYALFRSLGYRFDGVETAQIELASAPMRGQLVARGGVRVLVDCY
                     NANPENVRAGLAGFFALPTAGRRVVVLGDMLELGPESPRYHRESGAVLARYPFDLAVL
                     VGPRSRETLEGAVASGADRGRLRHCADAAGCAKEIAAIVQKNDLVYLKASRGIGLEAV
                     LNGLGEPGEEVL"
     gene            complement(78072..>78550)
                     /locus_tag="PKM94_01130"
     CDS             complement(78072..>78550)
                     /locus_tag="PKM94_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOD65268.1"
                     /translation="PFGVYVDFAHTPDALERLCQSAREMTAGRVLLVFGCGGDRDRGK
                     RPLMGTAATTMADYAVVTSDNPRTESPLAIIEDIRPGLRGDRYAIEPDRRKAIALALR
                     AAASGDVVLLAGKGAERYQEVNGERRPFDDVEEARAALAAMGYKKASAGANSTARK"
BASE COUNT        12969 a        26342 c        25480 g        13759 t
ORIGIN      
        1 cggcggctac gccgggaccg aaggtttcac cggcggcatc gcctttgatc cgcagcgcaa
       61 cctgctgtat tttgcctctc gtgcgtactc cggtgacttc ccgacagacg gtgtgttcta
      121 tcgcgtggcg gcgggcaccg gtatgctggc cggcccggct ctggcggcca cgcagaccgt
      181 gtactcgggc ccgacggtgg acgccaaccg cgtctacgtt gccggtaact cccgctacgt
      241 ggcggcgccg ttcggcggcc agatctacgc ggtcgacaaa gcgaccggcg tgcgcgtctg
      301 ggcggccaac gccggcccgt gggggtacat catcagcccg ccggctccga cgaccgcttc
      361 tctggacggc tgctacaaca gcgccgtgct gagctgcgag ccgaccgggg tggcggacct
      421 gctgtttgct ttcgggtcgt ttggcggcta ctacgtgtat aacgccgaga ccggcgacaa
      481 cctgtactac aaccgcgtgt cggtcgggac caacccggtc gcgcagcgcg gattcggcgg
      541 cgcgttgcag gtcgacgacg acggggcctc tcacctcctc tttgcggaca actccggcag
      601 actgtacgac ctgaccaagc agggcgaccg tccgcgcctg gagatccagc actacgacat
      661 gaaggtgccg tgtccgttcg gtaccgaccc gaactacctg ctgacgatcc cggatgtgta
      721 cacgaacacc ggttgcgccg acctccactt cacgaacgtg atcgtggatg agatctcggt
      781 ggccaaccag tggattcccg atatcgtggc gcagtatgtg ccggacgacg tcatgatgaa
      841 ggctgccggc atcgccgatc gcctgaccga tttccagttc tcgggcaagt tcctgcgtga
      901 agcggacgcg accgaggacg tggtgatcgc gagcgacgat ctggaacgcc gcacgaacta
      961 cgcggcggcc ggcgtcccgg cctggtttaa ctcgatgctg catccggcca tcagcgatgt
     1021 cctcatcgcc ggcgacacga tggatctgca gttctacgtg aaccaggcaa tgatcctccg
     1081 cggcgcgcag gacttctacc tgcaagtcga ttcggatgat ccggagttct tcctgaacga
     1141 cgtcaacaag ctgccggagc agcacgttgt ggtcgtgggc gggtgcttga ccgatacgac
     1201 gacgctgatt ttcggcgtcg gcggcgcgaa cgagcgtctg gtcagcaaca ccggccgcct
     1261 ggcgacgggc gactggggcg acggcccggc cggctacaat ggtatcacca tcgacggtga
     1321 cgtgagcagc tacttccagg gctcgtttgc cttcatgggc agcaagtacc aggtggcggc
     1381 gaacaccgac gactggggcg gcggcggcac ggccatggtg tcgatgcagg gcgacccgaa
     1441 ctggtgcgac gacgactgca agccggcgct tcagacgggt gtgaccctcg gctacatcac
     1501 cggcgacggt ggtctgacgt acgacccgat cctgggcaac cgcgtgtgct acaccatcct
     1561 cgactccgtg cagaatttcg atgccggcac gggctgggat tggacgtggt acgacgcgcc
     1621 gttcgacaat gccctgacca tgggtctgta cgcgaactgc cgcgcggtgg gtgcgctcga
     1681 cgtaccggag ttggccaaca tgaccatcga cgtgatggaa atcacggagc gcaatggcaa
     1741 tgcggtcgac ggctggtacc tgtcgcactt cttcgattgc gacaacggtt cggatacgat
     1801 ccagtacaac ggcacgtact ccgtggcctg gacgctggac ggcggcgccg cgcaagcgat
     1861 cgggaacgtg aagatcccgt tcggctgcgg ttacacgccg atcgtcaaca tctggggcac
     1921 ctacggggtg agcggctccc acggcttctg ggattggacc cagtactggg atacggcgta
     1981 ctggtactgc actcagggct acggcgccaa gactgaggcg accggcatgc acggcggcga
     2041 cgaagaagcg cacgtgaccc tggatcagca caacttcgct ccgaacgaaa cctatacggt
     2101 gggtattgcc acgttcgagt tgttcggtct gaccaattcg ttggatgcga acaatccgga
     2161 catcattgcg ctggcgaaga tcgccaacca gtgggccggt tttggccgcg gcgacgtgaa
     2221 caacgacggc gcgatcaacc tggcggacat tatctacctg gccggcacgg tcaacggcgg
     2281 tcctggggcg attccgttcc agcacctggc cgatgtcaat gcggacggca acatcgacgt
     2341 ggtcgatgtc gacttcctca tcgactacta cttcaactgc gggccgtgcc cgaccggcga
     2401 ctggatcttc taagattcag agagccgaag ttcttcatgg aagcggggcc gttcggcctc
     2461 gcttcctttt tttgttccgg gccgccgccg gcaggggtat aatacggcgt atggacccgc
     2521 gcgacttcga cagcgagacc gaggcgatct cccccctgct gcgcgatctc tacgagatca
     2581 acgccgtcta ccgtcagggg gaggaactgg ccttcagcct gtcatggcgc tacgcgccgg
     2641 tcaagtcgag gaagctgctc gaggaccgcc tgcgcacggc ggggtaccgc taccggctgg
     2701 cggagggcga ccagttgacg ctagtggtcg atccgcggcg gcggctccgc atccccccgc
     2761 tcaacatcat tctcttcgcc gcaactgtgc tctcggtcta cctgctgccg gtgctcctgg
     2821 ccaacgcggg ggcgccccac ctgtgggcgg cgacgctggc cgacctgcgg cggggggcgg
     2881 ggctccagtt caccgcggcc ctcatgtcga tcctgctcgt ccacgagatg gggcacttca
     2941 tcgcgagccg ccgccggcac atcgtcacat cctggccgta ttttctcccg gcccccaatc
     3001 tcctggggac attcggcgcc gtgatcaagt cgaagtcgcc gttctacaac cgccgcgacc
     3061 tggtggaagt cggcgcggcc ggcccgatcg cgggctgggt ggtggccctc gggtggctga
     3121 tcttcgggct gacgcagtcg cgctggatcc cggccggcct ggtggtgccg ggggagtggg
     3181 ctttcgggct ggagggggag tcgctcttga tgcgggcggc ggtgcaggcg ctggtcgggg
     3241 cgccggcccc ggagcacttt ctcaaactct cggagggggc gttcgcgggc tgggtggggc
     3301 tgttggtgac ggcgatgaat ctcctgccga tcgggcagtt cgacggcggg cacgtcctct
     3361 acgggttgtt ccggcgggcg cagcacgtcc tgggcatgat cggcctggcg gcgctgctcg
     3421 tgctcggcta cttctccccc ttctggtggc tcttcggcgc gttcggactg ctcttcggcg
     3481 tgaaacaccc gccgacgctc gacgacgacc ggccgctgag cccgaccgcg cggcgcatgg
     3541 gcatcgccgc catcgtcatc atggcgctgt cgtttacgcc gatgccgttt ccttctccct
     3601 tttagcgcgc gcctcgtcga tgaggcagga gacgtcttcg ctgcgcccgt acatccggcg
     3661 gaagtcgatc tggccctccc gcagaatttc caggaaaacg tcgacgatct cggggtcgaa
     3721 ctggcggccg cggtaggtga ggagttcgcg gacggcgacg gcgagggagt tgccgttgcg
     3781 gtagggacgg tccgagagga tggcgtcgaa ggtgtcggcg acggcgagga ggcggccctc
     3841 gacggggatc tcttcgcccc ggagcccctt gggatagccg gtgccgtcgt accactcgtg
     3901 gtgggccttg atgtacggga cggcgggttt gaagagctcg atgccgcgga tgatgcgcag
     3961 gccgacttcg gggtggctgc gcatctgggt ccgctcctcc tcggtcaggg cggtgggttt
     4021 gttgaggatg cggtcgggca cgccgatctt gccgatgtcg tggagggtgc agcccatgtg
     4081 gagatcgaag atgcgcgatt cgctccagcc gaggcggcgg gcgatcatct cggccagccg
     4141 ggagacgcgg tcggtgtgcc cggcggtgta cttgtcgcgg gcctcgatgg cgttggccag
     4201 gccgcggatc gcctcgaggt aggagcgccg caggtcgcgg tagagcttgt cgttggcgat
     4261 ggcggccgcc gccgagttgg tgagaatggc gaggacgtca agctgcccgg gggtgacgcg
     4321 cccgaacttc gacaccacca gcagggtgat cacgccgtgg aaacgctggc ggaccatgat
     4381 cggctggagg atggagaggc gcacctcccg cccggctttg gactgcgtcc ggcgggcctc
     4441 gatgaccggc tcggcccgtc cggcggagga gaacttctcc agcaccgtgg gatccagcgc
     4501 gagcggctca aggagcgggt catcgacgac acagagctta cgcagcaggt cgcccgaact
     4561 ggggtcgact tcgataatgc cggcggcgac ggcgccgagc tccttcttgc acgactcgat
     4621 cacgaggcgc aggtagcgtt cgatgtcaat gtgcgccttg tgggcgttgg ccactttcag
     4681 aaactcgacc tggccgcgga gccggacgtt ctcgcgggcg agacgctggt gctccagggc
     4741 gcgctcgatc gtcgatttga gcagttcgag cttgaagggc ttgacgagaa aatcataggc
     4801 gccgcggcgc agcacggaga tggcggtctg gacggtcggg tgggcggtca tcaggatcac
     4861 cacggcgtcg gggtggcgcg tgagagtcgc ttcgagcacc tgcgtgcccg aatggtcccc
     4921 catcaccaga tcggtcagga ccagatcgac cgggtgatcg tcgatgtggg caagcgccgc
     4981 gagcggatcg gagaacgccg cgacctggaa ttccggagcc gccagggcct cctcgatgat
     5041 gttgcagatg tactgctcgt cgtcgatcac cacaatgtgg gtcgtcggtc tctcagccat
     5101 ggatgccctc tttacagttt cagcggatct tctcctctgt tatcggacaa cggggcaaaa
     5161 ggcacagccg cccgccgaca atgttttatt tttgcgctga gcaagcggag gacagtcacc
     5221 gacagaattc gtcagctcga taggaggagc ggaccagcgg ggcagccgcc accagcggga
     5281 tccccgccgc ctcagcctcc tgccgcagtt cggcgaattc gccgggcgga aggtaccgga
     5341 cgaccgggtg gtgggccgcc gacggagcga ggtactggcc aatcgtcagc aggcgcacgc
     5401 cggcgccggc gagatcgtgg aagaccgtcc gaagttcctc gcgggtctcc cccagcccga
     5461 ccatgaggcc cgacttgaca gcaatagggg agcggcgccc cacttcggcc agcagcgcca
     5521 gcgatcgcgc gtagtccgcg ccggggcgca ctgccgggta gagccggggc acggtctcga
     5581 cgttatgatt gaagacgtcg gggcgggcgg cgatgatgat gtcgaccgcg ccggcaacgc
     5641 cccggaaatc gggcgtgagg atttccaccg ccgccgcggg gagccgcgcg cggagcgccg
     5701 cgaccacatc ggcgaaatga ccggcgccgc cgtcgggaag gtcgtcgcgc gtgaccgagg
     5761 tgacgaccac gtggcggagc tgcaggtcgg ccgccgcggc ggcgacgcgg gccggctcgg
     5821 tgggatcgag agggcgcggg cgccccggct cgatgtcgca gaagcggcag ttgcgcgtgc
     5881 aggtgggccc catgatgagg aaggtggcgg tgccgcggtt aaaacactcg ccgcggttgg
     5941 ggcaattcgc ggcatgacag accgtgttga gcccatagtg gcgcaggagc cgggagacgt
     6001 tttcgtaacc cattccggag aaggcggtta cgcggagcca ggcgggcttg cgcggaccgc
     6061 ctccgggggg gcgggagggt ggggagtctg ccataggttt cgccgcaatc agttctttac
     6121 caatcaagta taccgaaatt tgcacgataa ttcaagtaga cgtgtgcaaa caccgcaaag
     6181 gagagcctat gcgtcgtctg cttgtggttg tcgggtgtct gctccttccg gttctggcca
     6241 gcagtccgcg cgccgaacgc ggccaggccc gactggtgcc ggttcccgac ggcgctgccc
     6301 gcgccgaact ccgtcccgtc cccagtctgg ccaccgcaga tacctgcatc gttcgccacg
     6361 acgccggcat ttactataag atcgacggct gggtcaccgg caacgagttg tacaaggggt
     6421 actgcgaccc ggcgctcacc tgccccaacc cctacccgtt tatcgtgacc gaaatcaata
     6481 tgccgatgta tttcaccggg acgccgactt tcggcgtgtc cgtggatgtt gagctggcgg
     6541 acaactcgga tccgtcctgc ccgatgccgg gaccgctggt ggcgatttcg tcggagtaca
     6601 cgctgcaggt tccggccgag ggtctctacg acatctggat accgctggac acgccggtga
     6661 cggtaaccgg gccgttcttt gccgggttct tcctcagcac gccgttcgcg gaggccgacg
     6721 gcgcggcggt ggtgatcgac acgatcccgg agctgtgcgt ctcgtacaac atctgggaca
     6781 ccgcggtggg cttcatcgat ctgggcgaca acgaaatctg gaatttcccg ggccgcctcg
     6841 tgttgtacgc ggccggcgtg ccgatcggga cggccaatcc gccggacgtg cggctcctca
     6901 gcccgagacc atccgacacc ctgttcggcc cgaccgagat ctggatccag gagaagggcg
     6961 gttcgcccat tctcgattat gcggtgttcg agtaccgtcc cgccggcggc ggagcctacg
     7021 tcgagatcgg tcgggatttt gaccccaccg cggccttccg cgacggggcc tcggccacgg
     7081 cggccagcgc cgccggattg tcgtacgcgt tcaatttcgg cgcgctcacc gaggggatgt
     7141 ggacgctgcg cgcgacggtc tacgacaccc tcggccgctt cgaccgcgac agcgtcacgg
     7201 tctaccttga accgacgccg ccggtgccga cggtgatctc gcatgaggac ggcgggagtt
     7261 tctgcacgcc cctgacgttc aacctcgtct gctcggatga ggacgtcgac tacacccagg
     7321 ggttccgacg cggggcgctc gatgagtact cggccggcgt cattgtcgcc ggcccgcccg
     7381 cgcgcggtcc gcactggaat gccccttacg cggcggccgc ggccatcgag ctgtggtacg
     7441 accgggggac gacgcggccg atgaccgaga acaacctcaa gttgtcgctg gacaccctgg
     7501 cggcccgcct ggcctactgg atggaaacca cggtcaacac cggcacctgg gacgaggggt
     7561 ttctcgccgg gctcaatgaa tacctgcacg tcaaggggga cgacctctac accgcctatc
     7621 ggcgccgccc caactatttc cagatccgcg actgggcgga gaactgggag cggtcggtgc
     7681 tgctgggact ggccggcaca ccggggcagt gggtggtggt tgacgggttc tcgggatggc
     7741 tccaggtgga tggaacctat ctggtcagga tcatggaccc gctcaccggg acggcggcgc
     7801 cggtgagcct tcgtcacaac atggggaccg cggaggtgtt tgtcggcggc gcgtggcgcc
     7861 cggtggatct catggtatcg gtggcgacca aggcttggaa tccgacccgc tacgccatcg
     7921 gcgccgatct caacggcagc aacggctggt cgatctcctg gacgccctcg ggtctcaccg
     7981 aagacgacct ctatttcttc gacctggcag cgcgcgacgg ccacaacatg cggggcgtca
     8041 cgaccctgtt actgcagtac aactgcgccg ggaactacgc caagggagac tacaacaacg
     8101 acggggcgac ggatatcatt gacgccgact acctcctccg cttcgtagtg agcggcggtt
     8161 cggcgccgat tgggggggcc gggcgcgggg acgccaattg cgatggctac gtgaatttcg
     8221 ccgacgtggt gttcttcatg aattacctgt tcgggggcgc gagcgaaccg tgctattgac
     8281 ggtccgccgg tcatcgatcc gtgcgcagag ggtcagggcg acgagccctg gcccttttgt
     8341 gcggtcggaa cggcagccgc cggcgcggcg caccctcagg gacaggcggc cggcggcgga
     8401 ccgccgcgga acaggtagtt aatcagcgtg gtcagatcgg ccacgttcaa tatcccgctc
     8461 ccatcaatat tggcttcctc gtcgcacgcc ggcgcgggac cgccgcggaa cagaaacgcc
     8521 acgagcgcgg tcaggtcgga caccccgacc tcgtcctccg catcgccgtc gacgttgcca
     8581 cgcaggccga ggcagcagcc ggtcgacaca atcgcgacat cgtcaacata ccacccctca
     8641 tacgtgagcg ccccgtccga ggcaaaccga aacatcacct gggcgactcc ggcataggcg
     8701 ctcagatcga acaccgcctc cgaccattcc gcgtcgccgg agtaacacgg cgtcccgccg
     8761 ggaagggcgt tggcggggtt gtcgacgatc gtgtccgggt accccccctc ggggaagacc
     8821 tgctgccagg agccgccggc gaccgagatc atgacgatgg cgccgtccca ggcatcggtg
     8881 ggcgactcga tgttgcgcca gtgccagaaa ctcagcaccg cgttcggggg caggaggaag
     8941 ggcggactcg tcagcgccgc atccagggcg ttgtcgtaat agttgccgcc cgtcccgccg
     9001 actttccagc tcgtcgcgcc ggagtgcttc cggtaggtct cgcggtgcca ctggtcgccg
     9061 aagaactgtg tgtggggccg gtgccaccac cggttgtcat cgacctcgaa atcggacgcc
     9121 agtccggcgc tgttgccgat gaagatgcgg atcgagtcgg aggccgtgtg gccgccctcg
     9181 gccgccatgg tgacgtgcag gacagccacg tgcgggtcgg ggcagccggc atcgacagcg
     9241 agtttgagcg gcgtctgggt gaatcccgtc tcgccccagg caagcgtgtc gccgaggctc
     9301 gccgatgccg cctcgatcgc gatatacggg tcgtcggtgg aaagagtgac ggccggattg
     9361 accgcatccc agccgttgtt gaccacctgg atgttcaggt ccacggcctc gcccgggttg
     9421 ggacgcccgt tgccgtcgcc gcccgcctcg atgatctcct cgccgacttt ctgaagcacc
     9481 ggctgggtct gcggaaagat gtgggtgatc atgtactcct cgctggccgg gcagatgtcc
     9541 ccctcgatcc agccgcagta caggctgtcg aacacgtacc aggcgttgaa ggcatccgcc
     9601 cagccgtagt tcatgtggta ctcatagcgg cccgggatct gctcgcggta gccgtcgagg
     9661 acgatcgaat gcaggttgat gcggtactgg acgacccgcc cgctgtcgat ctcgttcttc
     9721 accaggtcga accattcgtg gagactgtag gcgctgcggt cgacgcgccg gacatcgggg
     9781 ctgaacttga aatactgcgg gtagatgagc acggcctgcc cggtgtaggc cgccgaaccg
     9841 caggaggagt agtccatgtc aaaggcgacg ccgacctcgt agcagagttc ggccacggcg
     9901 tcttcctggg ccggggtgca gccgaggctg cagctgtcga cgatgttcgc ccagtcgtag
     9961 gaatcggaat aggaggcggt gaggtagccg ccgccgtaat tgccgccgca atactggtcg
    10021 ccgtcccagt agtatgtgtg ggatccaacg ccgctcggcg gccactgcca gtaggcgatc
    10081 acctgcgagg ccgcggtggc cacgcagccg accacagagg tgccgtactg gccctgcggg
    10141 cagaatttgt tgtagggata cccctggtcc cagctggtgg tgcacagcgg cccgccgtcg
    10201 cgggtcgccc gggccgcctg agccgccagg tcggccccga attcgccagc cggaagggtg
    10261 aggcgggtcc actcggcttt ctggccgcgc ccgaacaggg cggggccctc gggcggctgc
    10321 gccgcctcga ggctgccgaa catctccgca tagaggcgcg tgcggttcgc cagttgttcg
    10381 gtgatcatgg cgagaaatcc gcccgactgc gagtcatcga ggttgtaccg gtcggaatac
    10441 gctttcaccg gcgtcatctc tttgagcgcc gcgaccagca cgaaaccgcg cgggctgatg
    10501 tcgtagtacc gggccagcag ggtgtcgccg accgcgagct cgtgggcgcc gagcacggtc
    10561 ggcgccgccg cgccggccca cgcgccccgg gtgaaaacca cttgggtcag ccagttttcg
    10621 cacgcgcgcg acatctccga cgacgaggcg cgttcggcgc gcacggtcgg ggcggttgtg
    10681 gcaaggagcg aggtggcggc gagaagaacc aggcggtgag aaaaacgagc ggcgcggtaa
    10741 gagagcttca tgtccatacc ccacgaaatc gagttatcag cggcggcccg ccgagtgtca
    10801 tataccgcaa gatagcaggt tttggggaaa atggaggaaa aacatgacga accgagagtc
    10861 gcgggaagag ccaactgcgg gccgaggccc ggtgacactg accacgcaaa ccacactgcc
    10921 tggagggagg tgacgtgtca aatcaagtgt cgttgacaat tagaaagcgg agagacaggg
    10981 attcgaaccc tggatagagc cattaagccc tataacggat tagcaatccg ccgccttcag
    11041 cctctcggcc atctctccgt tcaggattgc ggattcgtac ataggaatat atcggcgccc
    11101 cgccccccga tcaagccatt ttttctgccg ccccggcact ccccgccaca cactggccag
    11161 tcggcgcccg gccctccgct gggaacctgt cgtacggtca cgccttcggg acgccaccgg
    11221 agatcggttc cgccattgcc ggtcggctct gcaatcacgg gtcagtcctg ggatcgggcg
    11281 tggcgggatc cggcgcattt gccgaagccg cccattgggc caggctcgcg ggcgcgtggc
    11341 gcgagagccg ggcgcacagg gcgggcgttt cgatcagttc gagcagccgg tcggcgaggc
    11401 cggcgaaatt gttcggcgcc acgagcagcc cgttacggcc atcgcagatc actctcggga
    11461 tctccccgac cgcggtgctg acgacgcacc gcccgcaggc cagcgcttcc agcagcgaag
    11521 tcgggagggc gtcgatggtg gcgttgttga gatagatatc ggcggcggcc atgcggcggg
    11581 cgacctcggc atggtcgacc cggccggtga aggtcacgcc gtagatgcgc tcgcggcgga
    11641 caaactcctc cagccacggg cgcaacggac catcccccac gatcgtcagc tcggcccgcg
    11701 ggtatttctg tttgaccagg tggaacgcct tcagcgcgcc ggtcagaccg tttccgggcg
    11761 ccagccgtcg ggtcatgatg atgtgcggct gcacggcggt gacctgtttg gcagggaaca
    11821 actcggcatc gaccgtatcc gcccgcaccg cggcggtcag cccgtagcgg gagaacacct
    11881 ccgccagata cgcgcacgac acgaccaccc gatcgctcag ccgcaggaag gggataatcg
    11941 cccgcgcgta gtcgtccagc tcggtttccg ccttgttgct gcggtagaga acggtggtcc
    12001 ggacgccgaa gaatttcgcc agcagtagca cgggggcgac ctggtcgacg aacgagagat
    12061 cggagtggta agtgacggtc agcgaggcga agcgggggag cgaccggacc aggaggcgca
    12121 gtcccaggaa gtagtcgcgg agccagcgga agacggtggt gcgggcggca aaagccggga
    12181 gcgccatcga cagcgaaccg tcccggcccg cccgcagagt cgccggttgg ggctgggggc
    12241 ggcggtccgc gggggcggcc gccgcgaggt gcgggggcgc ggtcggcggg cgggctatcg
    12301 ccgcggccgg gcggccgact atgtatcctt gcgtcatcgc catcgctgct gctgcctccg
    12361 ctgagataag gaccgggacc gcgggggagc cggcccggta tttcttgtac gcgcgggggg
    12421 gcacgaagtt cacccggggg ccccgggggc cgcgccaggg cgagggcgcc cgaaacaatt
    12481 cgccggccct cccgtattgc ctgacgtaga agttgaacag cgacgcaaat ctcacgttac
    12541 agggagtcag gcccaatggt caagaaggtc ttcctcatca ccttcatcgt cgtcatcgtc
    12601 ggaggggttc tgtttttcgc catcgacggc tcggccaaga aggaggaggg gctgaaaacg
    12661 gtgccggtgg agcgggggga cattattgac aaggccctgg cggtagggcg gatcgtgccg
    12721 gagaaagaaa tcgcggtcaa gtccaagatc tccggcatcg tcaaacgccg ctacgtcgac
    12781 gtcggcgaaa ccgtggcggt cggcgacccg ctctttgaca tcgcccccga cccgacgccg
    12841 ctggagttcg cggagtccaa gcgccaggtc gagctcgcgc aggtgacgtt cgacaacgcc
    12901 aaacgggaat tcgaccgcac gcagtcgctc aaggacaaac agctcatctc caaccaggag
    12961 ttcgacaaag cgcgagccgc gtacgaggag gcggaacttc ggctcaaact ggccgaggag
    13021 cgcctctccc tcatccagtc ggggcggacc acggtcgccg acctgcaggt cgacaacacg
    13081 atcaaggccc ccatttcggg gaccgtgctc tccattctcg tcgaggaggg cgatccggtc
    13141 gttccgctca cttcctacca ggccggcacc gagctgatga cgatcgcccg catggacaac
    13201 ctcgtgttca agggggacgt cgacgaaatc gacgtcggca agctgcacga agacatgggg
    13261 gcggaaatcg aggtcggggc cctgcccaac agcaaagtca ccggccgcct cacgaagatc
    13321 tcccccaagg cctcgcagaa agaggggtcg acgctgttcg aggtggaaat cgctctgggc
    13381 gaagtgagcg acaccttcct ccgcgccggg tacagcgcca acgcggacat catcattacg
    13441 aagaaggagg ggatactgac ggtgccggag cggctggtca aaacagagga ctccgtctcg
    13501 accgtcgagg ttcaggatac tctcggtgtg gtgacgaagc gtgaggtcaa gacgggctta
    13561 tcggacggga tcaagatcga gatcaccgac ggccttgagg agggcgagct gctggtggag
    13621 cggccgccgc gcgagatcac cggcgactga ggcggacgga ggatgtgatg gtcccgctgc
    13681 aggtattccg ccagttcatg aacgacgtgc gccgccagaa gctgcggagc gccctgacca
    13741 tgttcggcat cttctggggg acctgcgcga tcgtcctgct gttcgctttc ggccagggca
    13801 tcggcgaggc gcagatcaag tcgcagcgcg ggctcggcga agacatcgcc atcttctggc
    13861 cgggaatcac ctcgaaggag taccgagggc tgccgcgcgg gcgccgtatc cgcccgaccg
    13921 aggacgacgt gctcctgatc aagcagaaag cgcaggcggt ccagcgcatt tcgccggagt
    13981 actcgcgctg gaacgcgccg atgaagtacg gccgcaacga gaccgtccgc aacatcgtgg
    14041 gcgtctggcc ggaattcggc gagatgcgca acctgatcgc cgacgtcggc tcgcggttcc
    14101 tcaacgggtc cgacatggcc gagcggcggc gcgtggtgtt cctcggcaac cggctcaaga
    14161 ccgacctgtt cggcgaccag gacgcggtcg gccgcgtggt cctcatcaac aacgtgccgt
    14221 tcacggtcgt cggcgtgatg aagtcgaaga agcaggacag ctcgtacaac gggcgcgacg
    14281 acagcaaggc ttttatcccg gcctcgacct ttcgcgcgat gttctcgatc cgctacctga
    14341 acgatttcgt cgtccagtcg taccccgacc ggacgatgaa agaggcccgg gaggagattt
    14401 acggaatcct gggggcgcgc taccggttcg atcccagcga caccgaggcg ctcatggtgt
    14461 gggacacgac cgaggggatg gcgtttctca agacgttttt cctcgccttc caggcgtttc
    14521 tggtcgggat cggcgcggcc acgctcatca ccggcgggat cggcgtctca aacatcatga
    14581 acgtcgtgct cgaagagcgg acgaaggaga ttgggatcaa gatggcgctg ggggcgcgca
    14641 agagcctgat tgtgtcgcag ttcgtgatgg agacgctgct catcaccggg ttcggcggcc
    14701 tgtgcgggtt cctcttcgcc gccgccatca tcgccgccgt gcccatgttc caggttgcag
    14761 actatatcgg cacgccgcgc atcgactggt tcggaagcct cgtggccacg ctgctcctcg
    14821 gtctggtcgg tttcatcgcc ggcatcttcc cggcgcgccg cgccgccaac ctccaacccg
    14881 tgcaggccct gaagctgttc tgagagcggc tatgatcaac ctcgcgaccg tctacaacgt
    14941 cttcatccgg gatttccgga agcagaagaa acggatcacg ctgacgctga tcgcgctcgg
    15001 ctgggggacg atctcgatcg tgctgctgct ggcgttcggc gagggactcc agcggcagat
    15061 gtcgatcaac ggcaaggggc tgggcgaggg catcgcgatc gtctggggcg gccagacaac
    15121 gatcccgttc aagggatacg gcaaagggcg cccgatctgg ctgaagaagg acgacgtgcg
    15181 gtacctggag gagcgcgtgc ccggactccg ctacatcggc ggggagtacc accgctgggg
    15241 gaccgagatc cgctacggcg acaaggtgct gagcgagcac gccaacggca ttccgcccaa
    15301 ctacgaattc atgcgttcgc acatccccat gcaggggggg cggatgatca acgacctcga
    15361 catgcggctc aaacgccggg tcgcctacct cggccacaat ctcaaggagc ggctgttcgg
    15421 cgacgaggat gcggtcggca agcagattct cgtcaagggg atccccttca ccatcgtcgg
    15481 cgtcgaaatc gagaagatgc agatgagttc ctacagcggc caggatgccg acaagctctc
    15541 tcttcccgcc accacgttcg aggcgatttt cggcgaccgc tacctcgaca acatcgtgta
    15601 ccagcccgag cgcgtcgagg acatgaaagc ggtggagaag gccgtgtacg aggcgatggc
    15661 ggcgaagtac acgttcgacc cggccgacga gcgcgctctg tgggtgtggg acgtcgccga
    15721 gtccgcccgg cagttcgaga acctcatgat cgggctgaag cttttcctcg gcattgtcgg
    15781 cggactgacg ctgctcatcg ccggggtcgg cgtggccaac atcatgtacg tgtcgatccg
    15841 ggagcggacg cgggagatcg gcatcaagat ggcggtgggg gcgcggcggt cgtacattct
    15901 cgcccagttc ctcatcgagg cgatcatcat cacgttcttc ggcggcttcg gcggcctggc
    15961 gatcagctac atcctcaccg agggtttcaa acgccttccc atcgaatcgg aggtgctgga
    16021 tttcatggga cggccgacca tctcgctgga cgtgggcgcg attgtcgtcg ccatcctcgg
    16081 cgtcatgggc ctgctgtcgg gctttttccc cgcgctgcgg gcggcctcgg tcaacccggt
    16141 ggagtcgctg cgctacgagt aaccattgga gacagggagc acaaaccaca tgctgatcgc
    16201 actgcacaac gtcgagaagg aataccgcac cggggcggtg tcgttcaagg ccctcaaggg
    16261 ggtgtcgctg gcggtcgacc ggggcgaata cctcgccatc gtcgggccct ccggctccgg
    16321 caagtcgacc ctcatgaatt tgatcggctg cctggatgtg ccgagcgcag gcgagtatct
    16381 tctcgacggc cggccggtcg gcgccatgac ctccaatcag ctcgccgaca tccgcaacca
    16441 aaccatcggc ttcgtcttcc aggcgttcaa cctgctgccc tacgccacgt cgtttgagaa
    16501 cgtcgaggtc cccctcctgt tcgcccgggt gccggcgcgg cagcgcaaac ggcgggtgct
    16561 cgagctgctt gcccgggtcg gcctcgccga caaagtcagc aacaagccga ccgagctctc
    16621 cggcggcgaa atgcagcgtg tggcgatcgc ccgcgcgctg gctaacgagc cggagatcat
    16681 cctcgccgac gagccgaccg gcaatctcga caccgcttcc ggaaaacaaa tcattgccct
    16741 gttcgacgag ctccgcaacg caggcaagac agtcatcatc atcacgcacg atgtcaacat
    16801 cgccaaacag gcgccgcgca ttgtccgcat ccgcgacggg gcaatcgagg agaacggcta
    16861 ccacccggtg ggggtgaacg tccaatccct ccagggttaa gcggctgcct ccgatgaccg
    16921 cctttttcgg aacaggctat ccggtcccga ttctgccggc gccttcagcc ggaattacca
    16981 aaggtattcg gcgagttgga cggcgaagat aaccagaaag agcaccagca gagtaacgcg
    17041 caccatcttg tagcccgggc ggtcgtgctc gggggcaggc cgatcgcctc ggcgaacccg
    17101 caggaacacg cgggcataga ggatcatcga aaaggcaatg agggcgagcg taatcggcgg
    17161 catcaccaac atgccgaacg ggaagcggat gacatagctg cccagcagga aggcgatcgc
    17221 ggtgaaagtg tatttcccgg tgatgttgga gccgaggacg accttccgcc cctgcagcag
    17281 gaagagcccg gcggccatga tgagcaggtc ccggccaaga atgaccaccg ccagccagag
    17341 cggaaaaccc cggtaggcga ccagcaggag taccaacacc ccggccatga tcttgtcggc
    17401 caccggatcg agcacccggc cgaggtcgct ggtgagcccc cgccgccgcg ccaggtagcc
    17461 gtcgagtccg tcagtcacag ccgccaggaa caggaaaccg acgcagacaa acggggcggc
    17521 cggggtgtcg agccacagga aatagccgat cagcggggtc atcgcgatcc gcgacagact
    17581 cagcaggttg ggcaggcgcc ctatttcacg ccaggaaatc acactctttt cgccacgttt
    17641 cgggttgtcc gggaaactaa cgccgccggt gaatcttcgc aaggggtatg aacggccgcc
    17701 gggcgggcta aagcattccg cgccgcggcc gataccacta ctacctcctt ttgagagact
    17761 ctttcgttaa cagttgcagg tgcgccgccg cgccccgccg ggcgcggcgt tcttctgccg
    17821 gcgggatcgc ttccccggcc ttcgcagtgc gcaactatcg cttgacaaac ggcacctttg
    17881 cctgcatgtt aaagtagtca tcggggatcg cccgaccctg ctcacacaag cgccgatccc
    17941 cttactcaaa ccatttatca tgagcgcgct ttcgcgcagg aggatactgt gatgatcgtg
    18001 ccgcgaatat tggcggcagc catcctcggc gtgctgttgc tcgcagcgcc ccaggcagca
    18061 gcacagctgt gcggcgatgt ggacaacagc gggggcgtca gccttggaga cctcgccgct
    18121 ctcgtcaact ggctcggcat tgagggtgcc ccgccgttgc ccaacccggc catggccgaa
    18181 atggacggcc gcgccggcat aacggtgggc gatctagtgc cgctgctcga atacgtcttc
    18241 gagggcgggc cggtccccac gtgcggcgcc acggatacct acaccttcgc cccttcgccg
    18301 ggcgacacgc tgtttatccc ccgcatgctc ggcattgcgg cggaagtcaa tgaggttacg
    18361 ctcccgctga cgatccacgt gcagtcgggg gtgcgggcct tcaacctgtc atttttggaa
    18421 aacaccgctg gaccggcctt ccgactggcc gacgtcacct accccccgga agtcgggctc
    18481 ctcctcgtgg gccgggacta cacgaatcac gattcgtgcc gggccatcgg ccttgagttc
    18541 aacccgaacc cattcacggg caccatcgtc gtcgccaatc tgatctatga gcgctattcg
    18601 tcgggagtgg ccgacatcat tcccgtcccg gtcaaccggg ccgatccgta ccgcaacgcc
    18661 gtggcgcgcg acggcgatct gtacgtgccg accgtcgcgt atctcgatgc agcccggccg
    18721 cctgacacgc tgttcgccgc tcccacggcg cttgatttcg acgccgtcta cgggtatccg
    18781 gccgtcgaca cgctgccggt cagtttcacc tcggcgccgg ctgcggtcga cttcacgatc
    18841 tccccggacg agagctggat acgggtcgtg aacggcgccg aatctccgtg gaccgccccg
    18901 gcaacggtgc aggtgacggt tgatcttgcc ggtctccccg gcggccttca caccgggcac
    18961 ctgaacatct ccaccaccga ccccgaggtc attctgccgg cgagcgccat cgaggtcaac
    19021 ctcacgatcc aggcgccggt cgacacgctg tttgtctcga ccgattcagt ccgcctggag
    19081 gcggtggcgg gcgtcccttc gtacccgatt gtctatgtga atttcagcca ctccctgaac
    19141 ccgctccagt tcaacctgtt gcccggccaa agctgggtga agctcgccga cccggccgcg
    19201 gcgccgttca cctcgcccgc gactgtgggg ctgaccgtca accataccgg cctgccgatc
    19261 ggggagtatg tcggggaaat gctggtcgtt ccggcagcgc ccggcgtcta tgtcgagcac
    19321 agccccatcc gcatgtatct gaaagtaacc ggactggctc tcccgccggg tgacctcaac
    19381 tgcgacgggg cggtggcggt tgccgacgtg acgatgctga tcaactacct gttccgcaac
    19441 ggtccgccgc tgacgccgtg ccagtaaggc ggctcagccc ccgaccgcaa ttcgcacaca
    19501 agccggccgc cgcgtcccac cgggcgcggc ggtttttgtc gccgcaagcg ccccggtttc
    19561 tcgttcgggc ggccgcccgg tcccccggct agtccgatcc ggacggcccg gctgtcccgg
    19621 aatctccccc gctctcctcg gaatgattgt actggtccat ccggtggcgg gcgatgatgc
    19681 cctcgaccat gtagatgacg tcttcggcga tgttggtggc gtggtcgccg atccgctcga
    19741 gctggcggga gacggcgagc atgtggatgt aagtttcgat ctggtcgggc tgggcgcgca
    19801 tgccggcctc gacctggcgg tacatctccc ggtggagccc gtcgatctcg tcgtcgcgga
    19861 gacacactcc ggcggcgagg tcggggttca tagagacgag ggagtcgatc gcccctttga
    19921 gcatgtcctt ggcgatctcc gccatgcggg ggaagtcgaa ggggaggacg accctgggcc
    19981 gggcggcgag gaagatcccc tgctcggcga tgccggcggc gagatctcct atgcgctcga
    20041 ggtcgctgtt gattttgagg acggcgataa ggaagcgcag atcgatcgcg accggctggt
    20101 ggagggcgag aattttcagg cagtcctcct cgacctccac ctccatgcgg tcgatctccc
    20161 agtccgactc gcacacgcgc agcgccatct cggagttgcg ttcggcgatc gatttcacgc
    20221 tcaggtggag gcgttcttcg accatcgcgc tgagcgacag gatcttcttc ttgatccgct
    20281 cgatttctct ctgcaggtgt cgggtcatgc gatcgggttc cctttttccg ggcgtgtgtg
    20341 gtttgcgtcg ggccgcctca gccgaatcgg ccggtgacgt agtcctcggt ccgcttgagc
    20401 gacggcttcg tgaatattct ccgcgtcggc ccgtactcga tcaggcgccc ctcgtagaag
    20461 aaggcggtgt agtcggagat gcgggccgcc tgctgcatgt tgtgggtgac gatgacaatc
    20521 gtgtagttgt cgcgcagatc gccgatcagg tcctcgatct tggtggtgga aatggggtcg
    20581 agggcggagg ccggctcgtc catcaacagc acctcgggcc ggaccgcgag cgcccgcgcg
    20641 atgcacagcc gctgctgctg cccgcccgag agcatgtagg cgctctgatc gagtttgtcc
    20701 ttcacctcgt cccacagaaa cgaccgccgg agcgcatcct cgaccgacgc ggcgacaatc
    20761 tgcctgtcgg tcacgccgtt gacccgcagg ccgtaggcga cgttgtcgaa caccgacttg
    20821 ggaaacgggt tggacttctg aaacaccatc cccacccgcg tccgcagggt ggagaggtcg
    20881 gcgatgtcgc ggtagatgtc gagaccgccc agccgcaccg tgccttccat gcgcgtgccc
    20941 gggatcgtat cgttcatccg gttgagggtg cgcaggaaag tcgacttgcc gcagccggag
    21001 ggtccgatga gcgcggtgac gcgcttctcc gcaatctgca ggttgatgtc gaagagcgcc
    21061 tggtggtggc cgtagaagaa gttctggtga tccacggtga tcttgcaggc ggtctcgccc
    21121 tcgggctcgg acggacgcgc gaaccgcacc tgcggcgcgc ggagcggatc ggccatcccc
    21181 gggcgggccg gttctccgtc gggcaaagca ggcggcgtcg gccgcggccg gttgtcggcg
    21241 gtgggcggtg tgtcttcagg cattcggacg cctcgcgcgc agtcgcgatc gaatgatgat
    21301 cgcggtgaag ttaaggacaa aggtcagcac cagcaacacc aaaacagtgc cgtagaggat
    21361 cggcttggtg gcctcgatgt cgggcgactg ggtcgccatg acgtagatgt ggtagccgag
    21421 ctccatgaac tggtcgttga gccgggtggg gaggtagggc aggtagtagg cggccccggt
    21481 gaacatgatc ggcgcgacct ccccggcgcc gcggctcacg gccaggatgc cgccggtgag
    21541 aatccccggc atcgcctgcg ggagaatgat ccgccggatc gtctgcagtt tggtcgcccc
    21601 gagcgcgtag ctggcgtcct tgagctctcc cgggatggtc cgcagcgcct cctcggtcga
    21661 gatgatcacc acgggaaggg tcagcagggc gagcgtggcc gccgcccaga cgatcgccgg
    21721 ctgtccccac accgggcccg gcgccccgcc atgaaacacc gcgtcgagcc cgccgccgac
    21781 gaacccgatg aaaaacccca gtccgaacag gccgaagacg atcgatggca ccccggcgag
    21841 attgctgatc gcgatgcgga tcagccgcgt gatcttcgag tcgggccgcg tgtattcgtg
    21901 caggtagacg gccgtcaaca ccccgatcgg caccaccgcg atcgtcatga gcagcaccag
    21961 cgccacggtc ccgaagatcg ccgggaaaat accccccgcc tgcatcccct gctccggcgg
    22021 atgggtaagg aaatcgaccg agaggacttc ccggccgccg atcagaatgt tggcgagaat
    22081 gacggccagc atgagcgcga taatcgcgac ggccgccagg gtcaaggact tcggaagcag
    22141 ccccgtgcct ttggctttca actgaaagtc gggcgccggc gggccgggcc gcgccgcgag
    22201 gccgcggggg acggcgctca tgacgtcctc ccctgcaggc gcgtcctgat ccggcgcagc
    22261 gccacgtcgc ccccgaggtt gatgaggaag gtgaaaacga acaggagggt tccgagcaga
    22321 aagagcacgc tgtagtgggc gctcccgaac acgacttcgg ccagttccgc ggcgatggtc
    22381 gcggagagcg tccgcaccga atcgccgggc gcgagcgaga cgaccgcggc gttccccgag
    22441 gccatcagca caatcatcgt ttccccgatc gcgcgcccga acccgaggat gacgccggcg
    22501 gcgatgcccg gcagggcggc cggaagcacg actgtccgcg agacctgcca ccggttggcc
    22561 ccgagcgcca gcgccgcgtc gcgcagcgac tgcggcaccg aagtcatcgc atcctccgcc
    22621 accgtgaaga caatcgggat gacggcgagg ccgagggcca ggccggcggt gatcgcgttc
    22681 agccggtaag tcaacccgag cgcgttctgc accaccgtcg ccaaaacaag cagggcgaag
    22741 aacccgagga caacggaggg aatgcccgcc agcagttcca ccaccggctt gatgaactcc
    22801 cgcaggcggc gggaggcgaa ctcggccgag tagatcgccg cccccacgcc gagcgggacg
    22861 gcgaatagca tggcgatgag cgccgccttc agcgtcccca caaacagcgg cagcagcgag
    22921 tacttcggcg tcccggactc cggctgccat ttgcacgacg cctcccgccc cgccttccac
    22981 tcctgcttga cgaccatttt ctccagcgtg acttcctcgc ggacggactc atcggtgaaa
    23041 atcgggacgg cttccttgaa gataaagatg aagatgagcg cgaccgagac cagggcggtg
    23101 agcgccgtga cctggatgag gcgctcgccc aggaactccc tgatcttcga tttcggtcgg
    23161 aactggtagg gctgcatact tactcacccg gcagcgcgcc gcggcgggcg ggccgatgcc
    23221 cgccgccgcg cggctattgc tttgctctct ccgccggcgg tcgtcgccgt ctcacggctc
    23281 acggaacctg gttggctctg gctctctcgg cgggcagcgg cacgtagtcg atgtcggccg
    23341 ccatggtctg accggcgggc gagagcaccc agttgagcag tttcttcagc tcgccggtcg
    23401 gggggccgtt gaagaaccag tagaggtcgc gcgaaatggg ataccggccg tccgagaccg
    23461 tctccagcga gggcatcacg gcggcggctg tatcgttttg ccgaaccgcc gcgaacttca
    23521 cgcccgtcgc ccacgccaga ccgccgtagc cgatcccgtt gggatccttc cccaccgcgt
    23581 tcaccaccgc ggccgtcccc ggaagcgtct gcgtccgatc ggcgtagtcc tcatcattca
    23641 ggacgtgctc cttgaagaac acgtaggtgc cggagttgtt ttcgcggccg tagagaatga
    23701 tcggagcgtc cggtccgccg acctctttcc agttgacgat ggcgccggtg tagatgcctt
    23761 tgagctgggg cacggtgagc tcgccgaccg ggttcttcgc attgagaaac acggcgatgc
    23821 cgtcgagcgc gaccgccacc cggaatggct ccttgccgtt cttttcggcc agttggtact
    23881 ctttctcctt catgtcgcgg gaggcctggc agatgtcggt cgatccgttg agcagggcgg
    23941 cgatccccgt gcccgagccg ccgccggaaa cctggatcac cgtccccgga ttttgcttca
    24001 tatactcctc ggcccagcgc tggccgaggc ggacgagcgt gtcggaccct ttcatcgtga
    24061 tggcgccggc gctcagcgcc actccggcca cgagcagcag ggcgccgccg atcagtacct
    24121 tcttcatcgt gttctctcct ctgccttcca tctggcgttc cgatgacata acatttgcat
    24181 tgttaggatc ttgtgaggat gcctcttgca cgggtgtgcc cctcagaatt tgagctgcag
    24241 gtcgacctgc agccggttta agtactcggc cttgtcggac gcaccgattc cgatggtgtt
    24301 atagaaatag gtggcgctga acgtagtgtt cccggccacc tggcaggcgg ctgagacctc
    24361 gtggccggag gcgttggcgc cgccctcgcc caagtcggaa tcggtgaacg ccccgacgac
    24421 ggcatcggcc tcgagctcgc ggtagacata ccgcaccgtc cacgagccgg gattcttgat
    24481 tttgccggcc gacgccccga gcgaccagcc ggcattgagg ctgtcggctt cgatgttgtt
    24541 cacgtagtgc ccgaacagcg ccgccggcac cgcgcccagg gggtgcgtca attccacgaa
    24601 cgcctccacg atgttgtagt catgcgcgta gacattgact gtctcgcccc cgaactgcgc
    24661 ttccaccgcg ctgttgccga aagcatcctc ttcgtcgtag aagagcggga aacctttgat
    24721 attctggtag tcaaagtagc cggcgccaac gagcaggccc gatttgtcct cgttgagaga
    24781 gagacgggcg gaaacctgcc cgctgccgag ccagctgtcc ttcgtcttcg atcgctcctg
    24841 gatccacaga ccggacacaa ccgcggccag agtgagcttt tcaaagttgt gctccagtgc
    24901 ggccgccgcc ccctccgggt tgaggtcgcc gtcccactgg agttcggaat ggccggggat
    24961 gatgaaaggc ttttccatct tgcccgccgt gagggtcagg aagctcaggc gtgcggggcg
    25021 atacatcagg gaggccaggt cgagccccag attcttggtc gtgaacgact ccccgagcgt
    25081 ctggttagtc gagaccggat catcggaacc tgaggccaac cccacgacca ctttggcggt
    25141 ctccgagacc cggccctcga tcgccaggcg cgcccgaagg cgctggcggt tgcgtgccgg
    25201 cgaagtatcg ctgttgcggt cgagcacttc atgacggtag cgcagatccc ctttgacctt
    25261 gacattgtct gtccactccg cggccgaggc ggcgccgacg acggaggcgg caagaaaaac
    25321 gattatccct ctcttcatgt tctcaagtcc ctttcacctt gcacggtttg cacaggggcc
    25381 ggacgtgcgg cgtccggttt ctctattcca tggcgacaga acgtactcag tgttaggcgg
    25441 aggttagcgc cgggttaatt ggccgcgaat tttctcgtca ccgtctccgc acctacgggc
    25501 gctgcacgat gacaagaggc tacggcgcaa tgaattaggc cgagtgcggc agatggatgc
    25561 tgaacgtgct cccctgtccg accgcgctgc gcaccgtcac ccgccccttg tgggcgcggg
    25621 cgatgtgttt gacgatggcc aaacccagcc cggttccccc ctggtcgcgg ctgcgcgcct
    25681 tgtcgatgcg atagaaccgc tcgaagatcc gctcgtggta gcgcggctcg atccccggcc
    25741 cctcatcggc gacgaacagc tcgacgccct cgaccgaccg gcgcgccccc gcccgcaccg
    25801 tgctgccggc cgggccgtag gtgatcgcat tgctcaggag attgacgagc gcctgctcca
    25861 gcaggctggg gttgatcggg gccacgagat cgaggtcgcc ctcgcacagg agcgcaatcc
    25921 ctttctcccg ggcgcgggat tcgcagctct gcaccgccga ccgcagcacg cctcccacgg
    25981 gccggcgtgt gacctcacac ccctcgccgt cgcgctcctg ctcaaggcgc gagagggcga
    26041 ggagatcctc gatgatgttg ttcaactggt cggcctggcg cttgatgatc tcaagaaagc
    26101 gccgcgcatc ctcgggccgg tccatcgccc cgtcgagcag cgtctcgaga aaacccttga
    26161 tcgaggtgac gggcgtgcgg agttcgtggg agacattggc gacgaaatct gtgcggatgt
    26221 tttccagacg gcgcaggcgg gtgatgtcgt tgagcacgat cacgccgccg gcggcgttgc
    26281 ccgacccgtc gcgcagcgtg gtgccgtgga cctgcagctg ccgctcgtcg tcgccggcca
    26341 attcgatcgt cccctccacc ggcgctccgg agtggagcgt ctccgagacc aggcggtgca
    26401 gttcgctgtt gcgcacaacc tcgggcacgc tccgcccgac ggcctcctcg ggccggatgc
    26461 ggagcagccg ggcggcggcg gcattcaggg tgaggatacg ttcggaggta tcgaccgcca
    26521 ggactccctc gaccatggcg gcgagaatgg cctcctgttc gttgcgccgg cggccgatcg
    26581 cctccatgcg ctcgtggagc tgggcggcca tttcgttcat cgcctgggcg agcccggcga
    26641 tttcctgatg cccggccgcc ggaagggcat agtcgaactc gccgcgcgcg aaccggtcgg
    26701 cgccggcgcg caggtcgccg atcgggcgca ccaccgaccg ggcaacgagg cggctgatca
    26761 cggccgcgag cagcaccaac agcagtccgc cgacgaccac atcaccggcc agcgtcccgg
    26821 tcaccgtccc gatcccccct tcggccgcgt cttccgggat ggccgtccgc accacgccga
    26881 ccacccggtc gtcggcaagg accggcaccg ccgtgtacag catgtggcgc tggagcgtgc
    26941 tgctgtagcg ccgggaggcc atcatctctc cgcgcagggc ggcggcaatc tccgggcgaa
    27001 accggtgctg ctccatgtgt ttcggatctt cctcgctgtc ggccagcacg cggccgtcgg
    27061 cccccacgac cgtgaaccgc gtccggccgg cgcggccgag cgcgaccacg agcgaatcga
    27121 gagcatcgtt gtcgtgcgca tccaggtagg gggtgatgac cctctccgcc agccgcgccc
    27181 gcatctccag atcgtggacg gcttcatggt aatgggcggt ccggtaagcc tgccgcgtgt
    27241 agaggtagac gccccccgcc gccagcgcca cgaccagtac aaacgagggg aacaactgcc
    27301 acaacatccg ccgctgccgc atcagttctc ccggaggcgg tagccgacgc cccgcacggt
    27361 ttcgatatac cggccggccg cgcccagttt cttgcgcaga ccgacaatct gcacatcgac
    27421 cgaccggtcg gtgacgatca catcctcgcc gcgcacctgg ttgacgatct ggtaccgcgt
    27481 gtagacccat ccgggccgcc gcgcgaggag gtgcaggatc tgaaattcgg tgtgcgtgag
    27541 ttggaccggg cggccgtcga cgctcacctc gtgccgcccg gggtcgatga tgaagccgtg
    27601 aatggccagc cgcgccgcgg cgtcctccgg cccggtcgcc ttgcgccgga gcactgcccg
    27661 gatgcgcgcg atgagcactt tggggctgaa cggcttggcg atatagtcat cggcccccat
    27721 ttcgagcccg gcgacgatgt cgctctcttc ccccttggca gtgagcatga caatcgggat
    27781 cgcgcgggtg gcggggctgt gcttcaactg gcggcatacc tccaaaccgt cgacgccggg
    27841 cagcatgagg tccagcaaca cgagcgccgg cggctcccgg cgggcgcagg ccaaaccgtc
    27901 ttcgccggtc accgcccgca cgacgcggta cccctcgcgg gcgaggttgt aggcaatcag
    27961 ctcctggatg tcctgttcat cttcgatgac gagaatcggt tccttcgcca tggcgcctct
    28021 ccaccggctg cgacggtcgc tttccgcact aaggtaaaac gcctcccccg gtaaggaaac
    28081 cactaacttt ccgttagact tgtgtgagga acgacggggc cccccgagga cgcgcgggtc
    28141 gccgaccgct caggcgtccg ggccgtgcag gccgtacttc ttcatccggt acaccagaac
    28201 gtgcctcggg attccgagct gcagcgcggc ccgcgaccga ttgccgccgg tgcgggtcag
    28261 cgccttgatg atgagttgct tctccgtctc cggcagggtg cgctccggtt ccgggtcgcc
    28321 cgcggcgggg cgggcctcgg ccgggccgac gtcgcggccg aaatccggag gcaaatccgc
    28381 ggtcgtgagc acctcgccgc gccggaggat cgccatgcgc tcgatcaggt tttcgagttc
    28441 gcggatattg cccggccagg cgtacgccgc cagccgcgcc atgagctcgg catcgaccgc
    28501 cagcggcgcg gagggggcga aagcgcgcgc gaactccttg acgagaatgg ggatatcctc
    28561 gcgccgctcg cgcaggctcg gcacccggag gggaatgacg ttgagccggt agtagagatc
    28621 ctcccggaat ctcccccggg cggctcgctc cttgaggtcc acattggtcg ccgcgatcag
    28681 gcggacatcc atctcgaccg gcttttccgc gccgaccggc tcgatcgttt tctcctgaat
    28741 cgcccggagg agcttggcct gcaggtcgat cgacaactca gcgatttcat cgagaaagag
    28801 cgtcccccgg tcggccagct cgaatttccc ccgcttgtcg cgcacggccc cggtgaacgc
    28861 cccccgcacg tgcccgaaca gctccgattc gatcagatcc ctggggatgg cggcgcagtt
    28921 gacgaccacc aactcccggt cggcccggtg gctgccgtag tggatcgccc gcgcgatcat
    28981 ctccttgccg gtgcccgact ctcccgcaat cagcaccgtc gcatcggtcg gcgcgatttt
    29041 ctccaccagc gccatcatct cccggaaggg gcgggagacg cccacgagtt tccgcattac
    29101 gtcgcgcccc cggagtcgtc cccgcaggag gcggttttct tcggcgagcc ggcgatgctc
    29161 gagggccttg tcgatggcca cgaacaactg ctcgtcctcg aacggcttgg tgagatagtc
    29221 gaaggcaccg agcttgacgg cctgcaccgc ctgggcgacc tcggcgtggg cgctgatgaa
    29281 aatcacctca agatccggct gtacgccgcg cacccgctcg agcagttcga tcccggacag
    29341 ccggggcatc ctgatgtccg agaggagcag gtcgaaacgc tcccgggtga ggcggtcgag
    29401 agcctcggcg ccgtcgccgg ccgccgtgac cgcgaacccc tgccgctcca gcttgaactg
    29461 gaggacgcgg cgcagtgagg cgtcgtcgtc ggccaaaaga atgcgcaccg tcatcgcacc
    29521 tcccccgcgg gcagccggag cgtgaacgtc gtccccgccc cgaccgtgct ctcgaccgcg
    29581 atcgacccgc cgtggctttc gacgatctgc ctggccacgg ccagcccgag accgctgccc
    29641 gaggatttcg tcgtgaagaa cgggtcgaaa atgcgcccga ctgcctccgg cgggatgccg
    29701 tggccgccgt ccgctatcgt gaggcgcgcg ccgccggcct cggattccac ggccacgcgc
    29761 acggcgcctc ccggggcgga cgcctcgagc gcattgagca gcaaattgag catgacctgg
    29821 tggatcttct cccggtcgcc gacgatggcg acgtccggct ggatgtgccg caccagccgg
    29881 acgccggcgt gggccgccgg ggcctcgatc tggcgcagtc cggccgccac cgtctccgac
    29941 agattcatcg gcgccaggtg cggctcgcgc gggcggccga actcgaggaa atcgcggagc
    30001 gtcccgtcga tccgccggat ctcgctcacc gcgatctcgc ggaactccgc ccggtccgcc
    30061 tccggcgtgt ccttgtcgca gatgatctcc accgccccct tgatgctggc gatcgggttc
    30121 ttgatctcgt gggccacggt cgaagccatc cggcccacca gcgcctggcg ctgcgaccgt
    30181 tcgagcgcga gttctgtctc ctgctggcgg cggcggaccc tgcgctcgcg gtcgacgatc
    30241 ccgccgatca ggccgccgag ggcgaagtag aagacgattt cagtgacctc tccggccgtg
    30301 ttgtgcgcgc cgaggtcgtt gacgaagatg aacgggagca cgagcagcga gatcgctgcc
    30361 gccgccccca accccccccg caggccgaac caggaggcgg ccaggacgat cgggatgtag
    30421 cacagccgtc cgtggatggc atggatccag tgctggggac caaaaatcgg ctccagcacc
    30481 cagccgtagt gaatgctgac cgtaaccagg atgatgccgc cgatgatcgc cagacggtag
    30541 cgcgttctct caggcagaga ttcagtgagc gagccggagc gcgggcccgc caaaggggag
    30601 ctttccggat gggcaggcgt cttctgcacg tttcctcctg aaaacggtat caccggcgga
    30661 ttggccggct tgtgatcgac cgatccatac accaacttat agtctcattc ggcattcttg
    30721 ttaacaatat tcttcacccg ttgaataata ttcttcaatg acttcattcg tcagccatac
    30781 attcaaatgt taagttgctg ctaatcatac cattatcagg tcggcacgtc ggttgttttg
    30841 tgagttggca tgaagagccg agtactccat gctcccacca gttggttcat cgtggcgctg
    30901 ctgctcgttc tatcgatctc cggagcgtcc cggggcgccg agccggcact ggccgacacg
    30961 gttctgccgg ccagcccggc tctcgacgat taccttcggg tcgccctcaa ccggagtccc
    31021 gatgtccgtc gggcctatga gagctggcgc gccgaagcgg ctcagacggg ttacgcggga
    31081 acgctgcccg acccgatgct gatgttcgag tacatggtcg atcccaaaca gccgcggctc
    31141 ggggcgcgcc aggagattcc gtggcttggc gggcttcggg ccgagaaggc gatggcggcg
    31201 gctgccgccc gggcggcgta cgaccgctgc gaggcggcgc gcctggctgt gatctaccgc
    31261 acgaccgcgg cctactacaa ctactggaac ctcagccggc aggttgcgct catgcgcgaa
    31321 aacctgcagc tcctcacgtt ctgggaggag gttgtccgca ccaagtaccg ggccgccctg
    31381 gcctcgcatc ccgatgtaat caaggcccag gtggagttgg ccgccatgga caaccaggtg
    31441 cactcgctcg aggatcgcct cgggccctcc cgacagcaac tgctcgcgga gctcaatctg
    31501 cccgccgcga ctgaacttcc cgtaccggcc ggcgtggttc ccccggcggt tactatcgac
    31561 gaaacgacgg cgctggccgc gattgactcg gccaatcccg accttcgggc gatggcggcc
    31621 atggtggagc aggagcgggc cggggtgcgg gcggcgaatg ccgcacggta tcccggcctg
    31681 accgtcggcg ccgactacct cttcgccggc cggtcagccg acccggggat gaccggcgac
    31741 ggtgcggaca cctgggtgct cagtgcatcc gtctccctcc ccctttggct cggcaagaac
    31801 caggcccggt cgcgcgcggc cgaggcccgc gaacgggccg ccgcttatga tctgaccgag
    31861 tcgcgcaacc ggctgcgtac atatgccgcc gacgtgatgt accagtaccg cgacgcgcgc
    31921 cgtcgggtcg agttgtacgc cggcggcctt ctgcccaaag ccgaggaggg gctcaatgcc
    31981 gcctacacag cctaccaggc cggggcgctc gattttctga gtctcctgga cgcccagcgg
    32041 caactgctcg aatttcaact ggcccacagc gaggctgtcg ccgccgccgc cgtccgcctg
    32101 gcggagcttg agatgctcgc cggcggccga ctcaccggcg agacgccggc gaccgaacgg
    32161 tgacatacga tgattccgaa aacttcgcac agagagaaca gctatgaacg attttgaggc
    32221 tgccggcggc cgctcccccc ggagccgaat cgaccggttc cgccgaaccc ggctcggcct
    32281 cgcggtgcag attgcgctgg tcatgggcgt gttcgcgctc ggcctgatga tcggcggggg
    32341 cgggagtccc gctccctccg ccgacagcgg gcgccccggc gccgaagagg cgaacgcccc
    32401 agtgctgtgg acctgctcca tgcacccgca gattcaactg cccaaaccgg ggaagtgccc
    32461 gatctgcttc atggatctta tcccggtcga gaccgggcgc ggcagcggcc gcctcggtcc
    32521 ccgccagatt gcgatgagcg aggcggcccg gaggctggcc cgcatcgaga ccgcgccggt
    32581 ccggcgcgcc gacgccgagg cggacattcg cctggtcggg cagatcgact atgacgaaac
    32641 ccgcctggcg tacattgcgg cccgcgtgcc cggccgtatt gacacgcttt tcgccgacta
    32701 caccggggtc accgtgcggc ggggggcgcc cctggtttcg ctctactcgc ccgatctttt
    32761 gtcaacccag caggagctct tgcaggcgaa gaaagcgctg gccgcagcgg cgcagagcgc
    32821 cggactggtg cgcgcgacag ccgaatcgac cctggccgca gcccgcgaac ggctccgtct
    32881 ctggggactg accgccgagc agatcgagcg tatcgaggcc ggcggccaac ccagcgacca
    32941 cttgacgatt ctctcgccca tcagcggcac ggtgatcgag aaagaagctc tcgaggggga
    33001 atatgtcgag accggcatgc gcatctatac ggtcgccgat ctctcccggc tgtgggtgat
    33061 tctggaagca tatgagtccg atctcccctg gctgcgcgag gggcagcggt tcaccttcac
    33121 tgcccaggcg ctacccggcg agacgttctc cgcggtcgta tcgttcatcg acccggtcat
    33181 cgacccggag agccggaccg cgcgcgtccg ggcggcggtg gacaacgctg cgggccggct
    33241 gaaaccgaag atgtttgtct cgggcgaggt tgcggcgcgg cttggctccg agagcggcct
    33301 cgcgcgctcc accggccggc cggacgcccc cctgctcatc cccacctcgg cgccgctggt
    33361 gaccggcacg cgggcggtgg tttacgtcaa aacggccgag gacggcgacg gcccggtgta
    33421 cgaagggcgc gacatagtcc tcggtccgcg ggcaggggat ttctatgtcg tcgccaacgg
    33481 gctggccgag ggagaggaag tggtctcgcg cggggcgttc aagatcgacg ccgagctgca
    33541 gattcaggcg aaaccttcga tgatgtcccc tgcgccgctc gcccgccggg cggccgacag
    33601 cgcgtctgtg cacggcgacc atcccgccgc cggcgcggac gtcgcgatct cgccggacgc
    33661 ccgggcatcc ctctccccgc tctacgaggc gtatttcgaa gtccagatgg ctctcgcgaa
    33721 ggatcaattc gaggcttcgc ggcgcggcct ggacgatctg gcggcgaaga cggcggcggt
    33781 tgacatgagc ctcttcgccg gccgggcaca cgcactgtgg atggacctgt cgcggcgcct
    33841 gcagaccgcc gcgcgggcgg gcgcggccgc cggcgacatc gccgccgtgc gcaacgcttt
    33901 ccgcgatctt tccgccaccg ccatcgacct gcaccgggcg ttcggtcacg ccgacgaccg
    33961 ccccttctac ctcacatact gcccgatggc tttccacaac accggcgcct actggctgca
    34021 aaccgaaggg attgtctgga actcctactg gggggccgcc atgctgcagt gcggggaaat
    34081 ccgcgacacg cttcccgcgc tctcggccgg agccgggatc accgcccctg actccggaca
    34141 gggccgctga cgtatgagca actcccgacc gcccgaccac agcgcgcgcg cgtcgctgct
    34201 ggaccgcctg atcggctcct gcctggacaa caagctggtc gtgatcctcg tcgccctggc
    34261 ggtgatcgcc tggggggtga tcgtcgcccc cttcgattgg cacatccccg gtctcgcccg
    34321 cgccccgatc ccggttgacg ccatccccga tatcggcgag aaccagcaga tcgtgttcac
    34381 cgagtggatg ggtcggtcgc cgcaggacgt cgaggaccag atcacctacc ccttgaccac
    34441 gacgctgctc ggcatccccg gcgtcaagac cgtccgcagc tactccatgt tcgggttttc
    34501 gacgatttac gtcatcttca aggaggatgt cgagttctac tggtcccgct cccgcgtgct
    34561 cgagaaactc aattcgcttc cctcggggac tctgcccgca ggcgtgcaac cgatgctcgg
    34621 acccgacgcc accgcgctcg gccaggtctt ctggtacacg cttgaggggc gcgacccgga
    34681 gggacgaccc gccggcgggt gggacctcga agagcttcgg tccattcagg acttccacgt
    34741 ccgctacgct ctccagtccg ccgacggggt ctcggaggtc gcgtcggtgg gcgggttcgt
    34801 gcgcgaatac cagatcgacg tcagtcccga cgccatgcgc gcggccggag tgacgctcga
    34861 agacgtgttc gaggcggtgc agaggagcaa catcgacgtc ggcgccggca cgatcgagat
    34921 caacaaggtc gagtacgcga tccgggggct tggcttcatc gaaaacctcg acgacctccg
    34981 cgcaacggta gtcaagtcgg tgcggggcgt cccggtccgc atcgccgacg tggccacggt
    35041 gcagctcggg ccggcgccgc ggcgcggggc gctggacaag ggaggcgcgg aggcggtcgg
    35101 cggcgtggta gtcgtgcgct tcggtgaaaa ccccctgcag gcgatcaaaa acgtcaaagc
    35161 caaaattgcg gaaatcgaac ccggtttgcc gaagaagacg ctccccgacg gcaccacgag
    35221 ccaggtgaag atcatcccct tctacgaccg caccgggctc atctacgaga cgctcgacac
    35281 cctcaacatg gcgctggtcg acgaaatcct ggtcaccatc atcgtcatcc tcttcttcgt
    35341 catgcacctg cgcagttccc tcctcatctc cggcatgctc cccctggccg tgctgatgac
    35401 cttcatcggg atgaaattgt tcggtgtcga cgccaacatc gtggccctct ccgggatcgc
    35461 catcgccatc ggcgagattg tcgacatggg ggtggtgttg tgcgagaaca tcctgcgcca
    35521 cctggacgag gcgccgcccg aggccaaccg ccgcgacgtc atctaccgcg cttcgaccga
    35581 agtcgggggc gccgtcctca cggcgatcgc cacgaccgtg gtctcgttcc tgccggtgtt
    35641 cacgctggag gcggcggagg ggaagttgtt tcagccgctg gcgtacacga agacctttgc
    35701 gctggtagcg tcgatcatga tcgcgctgac ggttcttccg ccgctggccc acctgctctt
    35761 cacgcgcggg cgccggtcgg gcgtttcgcg gcggctcctc cccctcggcc tggcggcggc
    35821 cgccgcggcc gccttcgtct ggcagctctg gctgaccggc ctcggcctgc tcgggctggc
    35881 cgcgttctac cggttgaggc accgactccc cgcgcgcgcc caggccgctg cgccccggta
    35941 tgcgaattac ctcctcctcg ccgtcgtcgg ctggatcacc gcgctccact gggagccgct
    36001 gggcgtcgag gccgggctgt ttctcaactt cctcttcgtg gcggtcctcc tcggcggcat
    36061 cctgctgctg ttccacctgt tgatccgcgc ctacgagccg atactccgct ggtgtctcgc
    36121 ccacaagaag atgttcctgg cctttcccgc ggccctcgtc ttgaccggtc tggtcatctg
    36181 gatcgggttc gacgcagttt tcggatttgt cccgcagttt gtcgaacgga cctctccgta
    36241 tgtggcgctc cggcacatgt tccccggcct cggccaggag ttcatgcccg atctcgatga
    36301 aggttcgttt ctctatatgc cgaccacgat gccgcacgcg tcgatcggcg aggccctcga
    36361 cgtgctccag acccaggaca tggcgttcag cgccattccc gagatcgaat cggtcgtggg
    36421 caagctcggc cgggcggagt cgccgctcga ccccgcgccg atctccatga ttgaaacggt
    36481 cattacctac aaaaccgaat acctgctcga tgcccgcggc cggcggatga aattccgcta
    36541 cgacgaggag aaggatgagt ttgtgcgcga ccagcgcggg caactgatcc ccgacgacgg
    36601 cgggcgcccc ttccgccagt ggcgggacgc catccactcg cccgacgaca tctggaagga
    36661 gatccaggag gcggggaagc tcatcgggac cacgtcggcg ccgaaactac agccgatcgc
    36721 cgcccgcgtg gtgatgcttc agtcgggcat gcgcgccccg atgggcgtga agatcaaagg
    36781 ccccgacatg gagacgatcg agcgggtcgg gctggaggtg gagcgaattc tcaaggaggt
    36841 gccgtcgatc gatcccgcca ccgtggtggc tgaccgcatc gtcgggcagc cctacctcga
    36901 aatcgcgatc gaccgcgccg cgatcgcccg ctgcggcctc cagattcggg acgtgcagga
    36961 cgtcatcgag gtggcgatcg gcggcctccc gctgaccacc acggtcgagg ggcgggcccg
    37021 ctacgcggtc cgggtgcggt accagcgcga gctgcgcggc tcgctcgagt cgctcgcgcg
    37081 catcctcgtg cccaccccgg cgggagccca aatccctctc ggtgagctgg ccgagatcgc
    37141 ctacgtgcgc gggccgatga tgatcaaatc ggaggacacc ttcctcgtcg gctacgtcat
    37201 cttcgacaag cggccggggc gggccgaggt ggaggtggtc gacgaggccc ggcgcaccct
    37261 cgcggccaga gcggccgccg gcgagttcga catcccggcc ggcgtcagct acgccttcgc
    37321 cggcagctat gagaatcagg tacgggcgga acacaagctg cgcatcgtcc tgccgctcac
    37381 cctgttcatc attttcatga ttctctattt ccagttccgc cgtctccccg cgaccttcct
    37441 cgtattctcc ggcatttttg tcgcctgggc cggcggcttc ctcatgctct ggctctatgg
    37501 ccaggactgg ttcctcaacg tctcgctgtt cggcgcgaac ctccgcgagg tgttccaggt
    37561 ccacccctac aatctctcgg tggcggtgtg ggtcgggttc ctcgctctgt tcggaatcgc
    37621 gaccgacgac ggcgtgatca tcagcaccta catcagccag gttctcgacc ggacccgccc
    37681 gacgacggcc gcggaggtgc gcgaggcggt ggtggccgcc ggcaagcgcc ggatccggcc
    37741 ggctctgatg acggtcgcca cgaccatcct cgcccttttg ccggtgctca cgtcaagcgg
    37801 caaaggctcg gacatcatgg tgccgatggc gattccgtcg ttcggcggca tgaccgtggt
    37861 catcatcacc gtcttcgtgg tgccggtgct ctattcggcg atcgacgagg cgcgggcggc
    37921 gcgggcccgg cggacgtgat tcacagccgg cggcgccgcc ggcgggcgtt cctcgccgct
    37981 tgcgttgcgg ctctgcgcaa gctaccttgg cgtgcgaccc ttgcctcccg gaggcccgca
    38041 gaagcgcgcc gtgctccgga cggttcgccg gacccgccgg ttcacatcga gatcagagga
    38101 cgcccatgcc cgattccgct cctgtcgaac gcccgatcaa ggtgggtatc agcacctgcc
    38161 tgctcggcca gaaggtccgt ttcgatgccg ggcacaagca cgatcgctac atcacggacg
    38221 tcctcggcgg ctactttcgc tttgtcccgg tgtgcccgga gctggaggtg ggaatgggcg
    38281 tcccgcgcga atcggtccgg ctggaggggg cgcccgacgc cccgcgcctg gtcggtaccc
    38341 ggagcggcgc cgactggacg gagcggatga accgctatgc gcgcgagcgc accgcccggc
    38401 ccgatctcgc cgacatctcc ggctacatcc tcaagaagga ctcccccagt tgcgggctcg
    38461 agcgggtgaa agtgtatccc ccctccggca tggccgagcg caaagggacc gggctgttcg
    38521 ccgccgccct gcggcagcgg ttcccgctgc tgccgatcga ggaggagggc cgcctcaacg
    38581 accatcgcct gcgcgagaac ttcatcgtcc gcgttttcgc ctacgaccgc ctgcagacgc
    38641 tgttccggga gcgtttcagc cgcgggcgtc tggtcgcctt ccacacggcc cacaagtatc
    38701 tcctcatggc gcacagcccg aagcactatg cggagctcgg ccggctcgtc gccgcggtga
    38761 gccggcactc gcccgccgcc atgcgggagc agtaccgccg gcttttcatg gaagccctcg
    38821 cggtcccgac caccacgcgc aagaacgtca acgtcctcca gcatctcctc gggtatctca
    38881 agcaccggct cggccccgag gagaagcagg atatcctgaa cgtcatcgcc gactatcatc
    38941 gggagctgat cccgctggtg gtcccgctca cgctgctgcg ccactacgtc gacaaacatc
    39001 gcatcgccta catccaggac caagtgtatc tgaacccgca tccgaaagag ctcatgctcc
    39061 ggaaccacgt gtgaggcatc tctccgcacg ctacctgcaa gaccgcggcg ccgattacgt
    39121 acggtgcctg cgcgggcagc tcgaccgccg catcgagctg acggtcggcg cggcgatccc
    39181 gaggtcccct cgggcggcga cgccgacctc gaaatccatc ctgcggatca tctgcccgca
    39241 tcggcgcaga ttgcggagcc ggcggcgatg ctgaaggcgg cggccgatgg gcggccgata
    39301 cccaatccgg tggatccccg gaggggctat tagggggcga cgacgcttcc gcggcaacac
    39361 taggaaattt tttcccacaa ccgcagtggc gcgtggatgg tcgatgcggg gactatgagg
    39421 gtcgatcttg gcaggtgcgg gtggggccgt attttcgacg ttgttgtttt ttccgccagg
    39481 cggcgttttt tttggtgaca accacttgtg cctgtctgta atttggcccc gtattgacca
    39541 acaacgcgga gagaaaacgg aaactggact ctctgccaac gtatcaagga ggtgctttgt
    39601 gatcaaacgg ttaacgcttc tcaccctggt cgcgctgctg gtcgcgccgg cgttctccaa
    39661 tgccaaggac atgaccggca agtacggcct cggctatttc acaactgacg ccccaatcgg
    39721 cttccggtat tgggccactc ccaaactggg tatcgatgtc ggagtagggt tcaagtccga
    39781 tgcggcttgg gtcggcgagt acgacgaaac cgaagacaga acggtgtact ccaaggagac
    39841 ggctagcagc ttctggttcg aggttggatt cccgtatgtg gtcgttgcga cggatcaggt
    39901 gaacttctgg gttcgcccgg gattccaatt cgcttcgttg gacgaccgca tcttcagccc
    39961 cgactcgaaa gtctatgatg cagatgacga cttcgaggaa caggggatgg acgatgtcgg
    40021 cctcgacgcc aagtttaccc agatgactat cagccttact cccgcagctg agtggttcat
    40081 tagcgacaat ttctcgattg aggccggcca cgggatcaag atccagatgc tcaagttccc
    40141 ggacgacaaa atccccggaa gcgacccggc cgagtactac tacggtgatg ctggcggcga
    40201 gaccgagacc agcatcgtga ccttctcagc cgacaatgtc ttcttcgcat ttcacttcta
    40261 cttcaagtag gccgtcccct tcgctgaggt gattagtgca aacgacacgg gcagggtcat
    40321 ccgatcctgc ccctttgtta tatttgtttg tcctcagaac cccgatcgcg gcgcccggtt
    40381 tttttgtagc tttgactgaa tactgaaagt agtttgacac ggaagtctgg ccataggcga
    40441 gaaccggaaa aggatagtca ggttcattac ccaacactca ggaggtgctc catgtttaag
    40501 cgtgctgcaa tgttcactct ggccttgttg ctgatcgtat ccatgcccac gtgggcccaa
    40561 aaccagataa aaccggtggt gtatgcgggc ggtggaattg acttgctcct gtcgcctgat
    40621 ttgctcaaag actactggaa gatgggatat ggattcggcg gcggcgtggg cattcaagtc
    40681 aagcctaacc ttgaactcat cggtcgcgtg tccttcaaca cttttccctt tgacgccgac
    40741 aaattcctgg acgacatgga tgccccggcg ggagtctctg tcgatgggct tgacttccgc
    40801 tcccttgagt tcggagccga tatgaagtat atcttcatgt ctgatcaagc gaatatgatg
    40861 ttcaagccat tcgtagtcgc cgggctcggc tttgcgaaca tcaaggccac ggatgcgacc
    40921 attacggctg aggacgagga tgacatagag gaagttcatt tgccttttgg cgatgccagc
    40981 gaaaccgatt tctcgtttaa cttcggcggg ggctttgact acatgttcgc gccaaaggcc
    41041 ggcgcctggg tggatgttcg ctacacctat atcgccagcg aaggagatgc catctcctac
    41101 ttgccgattc gtgcgggcgt gaagttcctc tttggcaact gattccctgt acgataggtc
    41161 cactgagaac cgggcttggc aattcgagcc cggttctttt gatacctccg cagcggcagg
    41221 tgggcttggg ctctctcgca acgactccgc acctgcacct taccacggtt tgtctgaaaa
    41281 caacagcgat tgcgaagcgc atgggtgaga ggttcaagat cgccggcgag tgtcttcacc
    41341 gggacggcag cgacctctcc cctacgggcg cctcgtggat cgcccattca aactgcccga
    41401 cagaaagccg ctaagcctgc tgtcgttcca atcaggctcg gcgaaatcgc ggcattgacc
    41461 ttatccttct gtcagcgtct cggccgaacg acagatggga gaagtggccg ccgcggctca
    41521 gattccgccc cgcggggacc ggtgccgcag tcagctcgcc ggcgtccagc cgcaggttat
    41581 cttcatcaac tgcgaactcc cagccctgcc cgctcccccc gccaatcgtc gactaaaatg
    41641 gacgaccggg taaaatatgt tgacagccgg gccgcggcac ggtagaattt cctcattcgt
    41701 gtacctctac cgcggcagag tccgaaggca gcctgcggca gtcggtccgg catcccggtt
    41761 agaggggtat tcggcatcgt ctctcaacac caggtaccca tagacgaaaa caccagcgtt
    41821 catcaccgaa gggtgccgct cttgtcgagg gcgccgcact agcggaggaa aggaggggaa
    41881 cgaaccaatt cgctccatgg tctgacgtcg tgccgactcg aagatctcgt cgggccggtt
    41941 caggggaatc accacttagt cgaaaggcag tgacttatga aagcaacgtt tgtggccgcc
    42001 tgtgtctgcc tgctgatcct gggcgcggcg ctgtccgcgc aggcgcagag tgaaaagccg
    42061 acgcttggtc tgggttacca cggaattctg gccggcgacg tcctgcaggg gctgagcggc
    42121 cgaatctgga catcgcccaa agtcggcctt gaggccaacc tctactacgc ccacgtgggc
    42181 gtggacgtga aaatggaggg tggggaaggg caggaagacg acgagtacga tatcagcggc
    42241 acgctgacgg acttttcggg caagttcctc tacgcggtag ccaatcacag ccagagcaag
    42301 ttctatgtcg gcgcggaact cggttacggc catgtgtcgg tcgaggtcga gaccccgggg
    42361 cttccggccg cgctggccga gggattcgac gtgatcacgc tcggcccgct catgggcgcc
    42421 gagttctcgt tcgccggaat ccccgagatg tcggttgact gggagctcgg ctatcgcacg
    42481 aatttggtcg atatcgaggt cgccgactac gacatctcgc tgtcgatggg cgtcctcgcg
    42541 gcctcggtcg gcgtgcacta ctacttcggc cgctagccgc actctgttcc aggttcgcgc
    42601 aacggagccc ggccctgccg ccgggctcct cgcaccggag agggcgggag cggcggcgct
    42661 tcactcgcag cagactcgga cgctctcgcg ctcgtcatcc acgtcgatac tcatctcccc
    42721 gagcgcgtcg atcagccgct cgagatcttc catgttcagc ttgcccaggt cgaccgtgaa
    42781 ccctttctcc gccagtgcct cgttgatccg ccccttcacg ctgtccggca ctacgccgcc
    42841 gagtttcacc ccggcccgga gcagagcgag ggggatccgg atattgacct gatcccgctg
    42901 cccctgtttc ggctgcacga ttacccggag gtatttgggc cgagcgccgg ccgtcctccc
    42961 cgtcgtctcc ggagcggtgc gctccgccgt ccggccgagc gcctcgagca gccgctcggc
    43021 ctcatcggcc gtgattttcc cttcggccag catgttgaga atgcggcgac gatcctcact
    43081 catgattctg ctccttctcc aattgcgaca acgcgtcctg aaccgtgatt tctccccgct
    43141 cgagccggtc gagcagttct tcccggcgat cgccgggctc gacctgcacc tgggcgaatg
    43201 acagcgcggc cccgatccgg ttcaaccggt tcttgaccgt cggatagctg atcccgaaca
    43261 cctcctccat cttcttgatc gaaccgtggc tgcgcacgaa agcggcgaca aacagctgat
    43321 cctcggccgt aagtttcgcc aacggcggca attcgaactc cccttccagc gcaatgccct
    43381 cgacgaccag ccgcacgcgc tccacgatca cttctttatc gccggtgagg cgggtcaatt
    43441 cctgccattc atgggccata tgtgtcttcc cattgtttca gttatttaat attgtatacg
    43501 atcaatagtc gtagaagtta atacgagaat tgattttcat tgtaagaatt tgtggattca
    43561 gacctctccg ctcatcaaca ggccttgttc tcaatcggca agccattatt ccccatttac
    43621 ttaagcgaga acgctctggg cagcgcatcc attaatgaag aagcggcgcg aaaaccgacc
    43681 catatcggtt ctcgcgccgc tgaagcaggc agagcgccgc cgagtatgcc tgtcctgcgg
    43741 gctaccggaa gatctcttca aagaacatct tcatttgctc ccacgaccgc cggtcggccg
    43801 cctcgttata cgcggcgccc ctcgacgcgt cgttgccggc gtccgggttg gtgaagctgt
    43861 gcaccgcccc gccgtactca gtcagttgcc agtcggcgcc gacccggcgc atttcctctt
    43921 cgaacgcggc cacctgcgaa tccggtacgt acggatcatc ggctccgccg agcacgagga
    43981 ttttggcctt gaccgtcccc ggctcggccg gctgagttgt cgagagaatg ccgtggaaac
    44041 tcaccacgcc gcggacgtcg gcccccgccc gcgccagctc cagcaccgcg gagccgccga
    44101 agcagtatcc gatcgcggcc agccggcgat catcggagag ttcctgcccg cgcagcacgg
    44161 cgagcgccgc ctgaacgcgg tcacggaaaa ggcggcggtc ctggtagtag gtctgggcca
    44221 gtttcgccgc ctcgtcggcg tttttcgcac ggatcccttt gccgtagacg tcggcggcga
    44281 aagccacata gccgagttcg gccagccgcc gggcgcgtat cttgacgtag tcgcccaacc
    44341 cggtccactc gtggaccacc agcacgccgg ggaccggtcc tgtgccgatc aggctggaat
    44401 cataggccag gtatccctcg aggacgacct tgccatgccg gtactcgacg gtttcggtgt
    44461 gcaccgcggc gccggccgcc gttgcccaaa gcagcgccgc acagaagatg aatgcagtcc
    44521 tcattattcg ggtcctttct cagggatgcg ccggcgaact gttcgccggg tccgcgcaca
    44581 aaaaacactg gccgttgact aatgtcaagg ctatcatagg cctattcagt cgatacttcg
    44641 cgcggcggtg cgtaccggcc cccgtgcccg gcgtccgcga caaactgtcc gccgcctgct
    44701 ccgtaatacg tgcggggaat caacccgcac cggggcggct gagccgacat ggcgaggtct
    44761 catatatgaa aggagctttg gtgtgaacta cttccgactg tggctcgcga tgggtgtggt
    44821 gattgtcgcc tccttcgcag tcctcggtta tttcggcggc gatatctatc gtcaggcccc
    44881 gccggtgccc gaccgggtcg tcacgtccga cggcgcggtg ctgttcaccg gcagcgacat
    44941 caaggatggg cagaacgtct ggcagtccac cggcggccag caggtgggca cggtctgggg
    45001 acacggctcg tacgtagccc ccgactggtc ggctgactgg ctgcaccggg aatcggagtg
    45061 gattctcaat gagtgggcgc gcaccgagta cggcgtgtcg gcctacacgt ccctgagcgg
    45121 ccccgatcag gcggctctcc gggagcgtct gcgcgaggaa atccgcccca acacgttcga
    45181 tccgaaaacc ggggacctgg tggtctcccc ggtgcgggcg cgcgccatcg aggcggtcgg
    45241 acggcactac gcgggtctat tcggcggcga tccggcgctc ggcaagctcc gcgaggccta
    45301 tgcgatcccc ggcaacgcgg tcaaggatcc tgcgcggctg gccaagctca acgctttctt
    45361 cttctgggcc tcgtgggcct gttcgaccaa ccggccgggc gagagcatca cctacacgca
    45421 gaactggccg ccggaggatc tcatcggcaa ccgcccgacc agctcgagca tcgtctggtc
    45481 ggtcatcagt ttcgtcctgc tgctcgcggg catcggcgcc ctcgcctggt actacgcggt
    45541 gctgaagcac aagccggtcg gggaggtggc gctcccggag caggacccgc tgctggcgct
    45601 ccagccgacg ccctccatga aagcgacgct caagtatttc tgggtggtga tcggcctgat
    45661 gattgtgcag atcatcatgg gaattctcac cgcccattac ggcgtcgagg gctccgggtt
    45721 ctacggcttc ccgctcgccg agtggctccc ttatgcggtc acgcgcacgt ggcacacgca
    45781 gctcggcatt ttctggatcg ccaccgcctg gctggcgacg gggctcttta tcgccccggc
    45841 ggtcagcggc tatgagccca agttccagcg cgccggcgtc aatttcctct tcgtctgcct
    45901 gctcattatc gtggtcggtt cgatgttcgg cacgtggtac ggcacgcgcc agcagatggg
    45961 tctgacggcc aacttctggt tcggccacca gggctacgag tatgtcgacc tcggccggtt
    46021 ctggcagatt ttcctgtttg tcgggctgtt cgtctggctg gggctgatgg gccgcggcct
    46081 gtggccggcg ctgcgcaaac ccggtcccaa ccgccacctg ctcgtgctct tctttatcgc
    46141 cagcgcggcg atcgccctat tctacggcgc cggcctcatg tggggacggc agaccaatct
    46201 cgcgatcgcc gagtactggc gctggtgggt ggtccacctg tgggtggaag gattcttcga
    46261 ggtgtttgca acggtggtga ttgcgttcct gttcacacgc atggggttgt tgcggacggc
    46321 gacggccacg gccggcgtgc tgttcgccac gaccattttc ctcgccggcg gaatcatcgg
    46381 gaccttccac cacctctatt tcacgggcac gccgacggcg gtgctcgcgc tgggagcgac
    46441 attcagtgcg ctcgaggtgg tgccgctggt gctgatcggt ttcgaggcct atgagaatct
    46501 ctcgctcagc cgcgccaaac cctggctcca ggcatacaag tggccgattt acgcgtttgt
    46561 cgcggtcgct ttctggaacc tggtcggcgc cggcctgttc gggttcttca tcaacccgcc
    46621 gatcgcgctg tactatatgc agggactgaa cacgacgccg gtccacggcc acaccgccct
    46681 cttcggggtg tacggcatgc tcggggtcgg tctgaccctc ttctgcctga aagggctggc
    46741 ctcgcgctac gcgtggaaga ccacgtggct ggcggtgggt ttctggtgca tcaacatcgg
    46801 gctcgccctg atggtgctct tgtccgacct gccgcgcggc ctcatgcaga cctgggcgag
    46861 tgtttcccac ggcatgtggt tcgcgcggtc cgccgaattc atgcagaccg gcctcatgga
    46921 tacgtttcgc tggctccgcg tgatcggcga tacgattttc gccgtgggcg ttttcgccct
    46981 cggctatttc gtcttcgggc tcaaattcgg atggtccgtg tcggggcagc ttgatcgccc
    47041 ggtcggcgcg gcgcccgaac ggccggaaga agtgacgagg ccttaaatct cacatgtccg
    47101 ccggcggggt cggaccggag cgcccgaccg cgccggcgat ggagggacac acatgttgcg
    47161 ctacaagtcc gggttctcga taagtctggc ggtgatgctg ctggtggcgg cgtcggccgc
    47221 cgctcactgc gaccgggaag acggtccggt cgccaacgac gcccgcgccg cgctcgccgg
    47281 cggcgagttc accaaaatcg ccgtctgggt gggcgaagcg cagaccgatg aattgcgccg
    47341 ggcattcgac caaacccgcg ccgtccggtc ccagagcgag gaggcgcgcg aactggccga
    47401 ccgctggttt atcgaaaccg ccgtccgcct gcaccgcgcg gccgaaggca tgccctacac
    47461 cggcctgcag cccgccggcc tccccgcccc cgccgaagtg gccgcggccg acaaggccta
    47521 cgccgccgac gacgtggagc ccgtgatcga gctgctcgaa tcgtcgctgg aggaacatgt
    47581 ccgcagcctc ttcaccgagg cgatgcgcgc caagaaggag ggagacaagt cactggaggc
    47641 ggggcgggag aagatcgacg cctacgtccg cttcattctc tacgtgcacg gcctggtcga
    47701 gaaaatcgag gccgggccgg agcacggcgt gggcgaacac tcgtcggtcc ccgacccgca
    47761 caatacgaaa ggagcaacta tgaataccgt ttctgccgac cggacggtcg gcgatctggt
    47821 caccgaacag cccggccgcg ccaaggtctt tgaggatctc ggcatcgact actgctgcgg
    47881 cgggcaccgg ccgctgaccg aggcctgcgc ggagaagggg atcgaccctg aaaacgttct
    47941 cgctcaactc gcccgggtcg atgcggcgcg gcgccccgac gccgacgcgg actatggttc
    48001 gatgagtctc acagcgctgg ccgatcacat cgtcgacacc catcacgcct acctgcgcca
    48061 ggcgctgccg cgcgcgaacc aactggccgc gaaagtcgcc caggcccacg ccgcctcgcc
    48121 caaaggcgcc gacctgaagc gcgtcgacga gcacgtgcgc gccctggccg ccgaactcca
    48181 gtcgcacatg gtgaaggagg agcacattct ctttcccatg atccggcgca tcgacgccgg
    48241 ggagcgcggc ctggtgatcg acggtccggt gatggtcatg atgcgggagc acgacgaggc
    48301 gggcgaggcg ctcgccgcca ttcgcacgct caccgatgat ttcgccctcc ccgcgtgggg
    48361 atgcaacacc taccgggcga tgcttgacgc cctccaggag ctggagctgg atctccacca
    48421 gcacattcac aaggagaaca acgtcctctt tcctcgggcg cagcaggcgg ccgccgtcgg
    48481 cgcctgaacg ctcacacagc cgacaccggc gccggcggcc gatccgccgg cgccgttttt
    48541 gttctttcgc ggcccggtcg gtcccatccc cgccgcgttt tctgcaacaa ccggcccgcg
    48601 cgtttcgtat actggcggaa accgctcgac ggaggagata tgaaccgtcc gctcgtggtt
    48661 cttcttgcgc tcgcctgcgc cgtcgcaccc gcggtcgcgt ccgcggatcc cgccgctccg
    48721 acggctctgc ccgatactcc cgccggccgc tgtctggccg aattcgtgtc cgcactgaac
    48781 agcagcgaca gcgcgcagtg gattgccttt ttggcccagc atccgccggc ggatgcgaat
    48841 gacagcgccg ccatccagga gcgccgtttc gggctgatgc ggacggtcca cggcgatttc
    48901 ggcgggctcg acccggtcaa ggttctcgaa tcgtcggagt actttctctc cgtcatcctg
    48961 cgcggccgcg tccccgtcca ggcctccggc tatctccgtc tcgccttcac cgtcaccgac
    49021 cagcctcccc accggtgggt ccggatggaa gccgagatgc tcgatgaaga cccgttcgcc
    49081 ccgccgcccc cgcgcatgga atccgacgag cagctcgccg cctgggtcga cagcgtggtc
    49141 acggcgatgg ccgccgccgg gcagttttcc ggcgcgattc tcctggccaa ggacggcgtc
    49201 cccttctact cccgcgccgt cggtccggcc gaccgctgct gcggcgcgcc caacctcctc
    49261 gacaccaaat tcaacctcgg ctcaatgaac aagatgttca ccggcgtggc catcgcccgg
    49321 ctcgcccagg agggaaagct ggccttcgag gatccgctca tcaggtacct tcccgactat
    49381 cccgaccgcg aggcggccgc gaaggtcacg atccaccagc tcctcaccca caccgccggc
    49441 gttgacgact actgggagga gctcttcgac accagtttct gggagatcaa atcggtcgac
    49501 ggttactatc aactgatcaa ggacaagccg ctgcgttttg agcccggcac gcagtaccgg
    49561 tactccaacg gcggtccgat cctcctcggt gttatcatcg agcggatcac cgggcagtcc
    49621 tactacgact acgtgcgcga tcacatcttc cggccggcgg ggatgatcaa caccgactgc
    49681 tacgaggtcg atgtcccgac gcccaacctg gcgattggtt acacccgcct tgactacagc
    49741 ggccgccggg acacggtgtg gcgcgccaac tacttcatgc atgccgtcaa aggcggcccg
    49801 gccggcggcg gctactcgac cgtcgaggat ctgctggctt tcgaccgggc gctgcgctcg
    49861 taccggctgc tgaaccaggc cttcaccgac accgtcctcg ccggccgcgt ggccatggag
    49921 cccggtcccg tgagcacgcg caaggcggac gagatgtacg cgtacctctt cggcgacatg
    49981 aggaccaacg gtcaccgcat cgtgggccac tccggcggcg cccccggcat caacgccgtg
    50041 ctcgaaatgt actgggacga cggctacacg gtggcggtga tggcgaacat cgacgaagcg
    50101 gcgtcgacgc tcgccgcgcg catccgccgc gtcctgacgc agccgtaggc ccggccccgc
    50161 ccccgagcgt ccccttcctc ccgcggcgac ggacccgcgg gcgcggtcac tttaatcttg
    50221 acctcgcccg gcccctgcct accttacctc ccagacccgc atctgatgaa aggaacgcca
    50281 tggatgacgt gacccttctc tctcgagtcc agttcggcct caccgtcggt ttccactaca
    50341 tcttcccggt tctgagcctc ggtctcggtc tcttcctggt gatcgccgag ggcatctacc
    50401 tcaagtcgag ggatccgctc tgggagagca tcacgcgctt ctgggtgcgc attttcgggc
    50461 tcattttcgc cctcggggta gcgaccggga tcgtcatgga attccagttc ggcaccaatt
    50521 ggtcggtcta ctcgcggttt gtgggcgacg tgttcggcag cgccctggcg gccgaaggga
    50581 tcttcgcgtt tttcctcgag tcgggcttcc tggcgatcct ccttttcgga tgggatcggg
    50641 tcaagccgcg cacccatttc atcgccaccc tcttggtcgc gctcggcgca cacttctcgg
    50701 cggtctggat cgtggtggcg aattcctggc agcagacgcc ggccggccac catctcgtct
    50761 cgcacgcgca ggggatgcgg gcggaggtgg tcgatttctg gcaggtcatc ttcaacccgt
    50821 cgtttgtcga ccgcatcacg cacgtcttca tgggggcgtg gcaggcgggg gcgttcctcg
    50881 tcctgagcgt cagcgcctgg tacctcctgc gtcggcggca ccgcgcggcg gcggtgggtt
    50941 cgatcaagat tgcggtcgtc atcgcagcgg tcgcctcgct cgcccagttg gtgagcggcc
    51001 acagctccgc gaacgttgtc gcccgccacc agccggccaa gctggcggct ttcgagggcc
    51061 attaccccga gtccgctccc ggcgattcct acctcttcgg ctgggtcgac gaagccggcg
    51121 agatagtgta cggaccgaag attcccggcc tgctgtcgtt cctcgcctac tcggattttg
    51181 acgctccggt gaccggcctg cgcgccttcg cccccgagga ccgtccgccg gtcaacctca
    51241 ccttccaatc gttccacctg atggtggcga tcggcttggc gctcattgcg atcagcctgg
    51301 ccgcggcgtg gtactggcgc cgcggcacgc tctgggagaa gcgctggttt ctgtggatcc
    51361 tggtgctcgc ggtggtgctc ccggagctgg ccaaccagct cggctggttc tccgccgagg
    51421 tcggccgcca gccgtggatc gtgtacgggc tgctgcggac gagcgcggcg gccagcgtcg
    51481 gcatcggcgg cgcccacgtg tgggggtcgc tcgtgatgtt cgccgtgatc tacatcctgc
    51541 tcggcgcgct gttcatctac ctgctcaacg acaagattca gcacggtccc gacggcgcgg
    51601 agccgccggc cccgtccggt agtgaacccg taatcgcgaa ggagggcccc cgcgcatgat
    51661 gaccgatctc aacagcctct ggttcgccct ggtcgggctc ctctttatcg gctacgccat
    51721 cctcgacggt ttcgatctcg gcgtcggcgc gctgcacctc ttcacgcaca ccgacgagga
    51781 gcggcgggtc atgatcaacg cgatcgggcc ggtgtgggac ggcaacgagg tctggctggt
    51841 gaccggcggc ggcgctctgt tcgccgcctt tccggaggtc tacgcgaccg ccttctccgg
    51901 gttctatgtc ccgttcatgc tgctgctctt tgcgctcatt ctccgcgccg tcgccatcga
    51961 gttccgttcc aagcgggcgt cgcacgcctg gcgccggacg tgggacgtca gctttacggc
    52021 cgcctcgctc ctcggcagcc tcctgatcgg cgtggcgctc ggcaacatcg cctggggcat
    52081 ccctctcggc cccggctacg agtacctggg ttcgttctgg gatctgctca atccctacgc
    52141 cctgctggtc ggggtcacca cggtcgcgct gttcatgatg cacggggcga tctacctgac
    52201 gctcaagacc gagggggcga ttcatgacaa ggtgcgcggc tggatcaaca acacgatcat
    52261 tttcttcatc atctgctacg ccacgacgac catggcgacg ctgttgttcg tgccgcacat
    52321 gaccgacaac atccgcaacg acccggtcct gttcgtcctg ccgctcctga acatgctggc
    52381 gatcgccaac atcccccgcg agatccatca cggccacgat ttccgcgcct ttctctcgag
    52441 cgcggcggcg atgttcttcc tgctcgtgct gttcggcgtc gacatgtacc cgcgcctgat
    52501 cctctccagc ccgcttaccg agcacagcct gaccatctgg aacgccgcct cctccgaccg
    52561 gacgctcggc atcatgggga tcatcgccct catcggcctc ccgctggtgc tgacctacac
    52621 gatcacgatt tactgggtat tccgggggaa agtgcggctc gaccggagca gttattgagc
    52681 gcacctccgc gccccggcgg gccttcagcc ctgcccggcg aacgccgata gaaagggcag
    52741 gggcggcaag cccgacaagc cccgacggag gagactgtga acgccaaatc tctggtgatg
    52801 gtgattgcgc tgtgggcggc ggcggcggcg gcctcggccc aggaactgct tcccccggac
    52861 acgaccgtca cggtcgccga catcatgaac cgcatggcgg ctctgatggg cgggccggag
    52921 tcggtcggcg gcatccggac ggcgcacatc gtcggccatt cccgcctgcc caattccgac
    52981 cgcgccgcca caatcgaagt ctggtgggca cagccgaacc tgttccgcgc ggtgacccgg
    53041 ccgcctgtcg gcgacgctgc ggaatgcggt tttgacggcc gcgtgtgctg gcagagagaa
    53101 cccaagaccg gcccgcgaat aatcagcggc gaggaggcgg gcgatctgat tctcgagtac
    53161 caccccggcc ggtttatcct ctggcgcgac ctctacctca acgccgatct gatcggcgcc
    53221 gacagcgttt tcggcgaact ctgcgacatt gtgcgcctcg tcccggacca gggccgcgcc
    53281 caggacgtct acctcagccg ctcagcggga ctccccgtca tgatcaagac cgagcgggcc
    53341 atgggcgccc gtctcgtcgc cgtgaatatc tacctctcgg actaccgcgc cacgggcggg
    53401 accgtgctcc cctggcgctc gctcatggag ttttccggcc gcacccggac gatgcaggtc
    53461 gacaaagtca gcctcaacgg cgacccccct gcggacatct tcagcctccc cgacgatgtg
    53521 cgcgtgctcg cggcggctcc cgatgatccc cgatagcttc tccctttcgg gcccgaaaaa
    53581 ctgcggccgc gcccgggatt ccgcgcgcgg ccgctttttc tttcacccgc cggtcagccg
    53641 gccggtctat tctttcgtca tgagggcctt gatgtcctcc ggaagatcaa acagccccgg
    53701 tttgagctcg acattgtgcg ccacgctgtc aaccttgatc gcacgcgtct gccccatcac
    53761 atccatggtc gtcaggaagg gcatcttcat cccgtcgaca ttgcggtaat cagtcatgta
    53821 gacggtgatg ggaatctgcc ccatttggtg ttcgacggtc atgaccattt ttaggggcag
    53881 gccggagtcg cggctgatcg agagtttttc cggctgcgag ccctcgccgg tgagcgccac
    53941 cacatcgcac acctcgccat tgaccgtgtc gaccccggcg agttcggcgc tctggtagat
    54001 ctggcgccag acggcgtact tgtcgaagtg ggcatcgcgc agccgctcgg cggcttccgc
    54061 tccctccagt acgcgcgggc cggtcatgac cgagcgttcc caggcggtga cgccgtcgca
    54121 gccgctctcg atggagccgg tcgcttccga ggtagccacg gcaaactgga ggttgggttt
    54181 cgccgaatag gtttcgatgc tcaggctgag tcccatgccg gcgatttcga gcgtgctcct
    54241 ggagtagcga ttgttgattt tggcgagcgt ttccagaccg ccggtggctt caaccatgcg
    54301 gtccatgagg gcggccgctt ccggagtaat gcctgcgggg gccgccgcct gcgccggggg
    54361 aaccggctcc tgtgcggcgg cgaggccgag gccggcccac aagagcacgc cggccagaac
    54421 gacggtaaat cccaactgtt tcatcatcga tagctccttt agttcagccg ttcacttttc
    54481 ctgttgccgt atccagtcgg cggcggcctc gatcacccgg tcgcgcccgg ccagcaggct
    54541 ctcgcgggtc agttcgaccg gctcgtcggg cgttacaccc gtcccttcga gacgggtgcc
    54601 gtcggccgat agaaaatccg caaacacgta caggagcgca tcgccgttgg cgagcttttc
    54661 ggtcatcgcc gagagcgccg cccccatcga ccgggagccg aacacgcgcg cccgcccgat
    54721 cgcctgaagg ccccccgaga agaactccgc gctcgagccc gagagcccgt cgatcagcac
    54781 cgcgaccctg ccgtcgtaag tctctgcgcg ggggttgatc accatcttga ggtttgactc
    54841 gcgggtggtc agagtgccga tgagcaggtt tttttcgctg accatgaatc cggccagacc
    54901 catggcgatc ccgccaatgc caccggggtt gcctcgcaca tcaataatcc accccggggc
    54961 gtcgcggtgg gcggccacaa attcctggaa acgccccatc agcattcccg ggtcgaaaaa
    55021 gcagttgaag gcaaagtagc cgatcccccc ttcaagcgtc gccgtgtcaa tggtcaggta
    55081 gaacggcggg aggttgccaa acacggtgcg ctgtccgacc cgttccgcca ggacgaggct
    55141 gtcggtgacg aggttcccgg aactatcgcg gaaggtcgcc gtcaccgtgt cgccgacctt
    55201 gccggcgagg cggttgagca cggcggtggc ggcatagatg ggatagcgga tatggtccgc
    55261 gtactgctcc tgcagcggcg ccaacacttc cgtcaccttg tcgccgccgg ccgtgaccac
    55321 ttcccagccc ggccggatcc cagcctcggc ggcgggtgtt tccggccgca cgcgcgccac
    55381 cagcgccgca ccgccgacca cccggaccac cattccggtg acccccgcct ggtccaccgg
    55441 gttgtccggg atgtccatgg tggagagaat ctcgcccggg agaatttcaa aatggctcag
    55501 cccgaggcgg tgaatcatct ccgccagcag cacccggaca tcggccatgg tttcgcaggt
    55561 gtccagcttc ggccgcagtt cgatcctcaa gcccggccag tcgacgcccc cgaacgtggt
    55621 atcgtagtgg cggtcgtaga caatctgcca cacctggtcg aacgactgct cgcacaggcg
    55681 gcgctgctcg ggtgtgagct gatcgccgcc atcggcggcc gccgcgggca ggcaaaacgc
    55741 cagcgccgcc aggacacaga acgcccgcac tcgcttcatc gtgtcatctc ctcatgtgac
    55801 atattacgcg gcccacggcc gggtattcga ccggagcagc ggccgcaccg aaaccgctcc
    55861 gggccgattt caggttgtct aaatgtcaac ctccacgccc cggtcccgca gcgcctgcac
    55921 ctggccgttg gtggaggggt cggggatggg attgaatttg agatagacgg catccccttg
    55981 ccccacgccg ccgttgtcga ccagcggctg gaggtcggtg atcgcattgt agttcaagtg
    56041 aatgatgcgg aggttggcca ggttacgcag cggcgacagg tcgacaatgt tgtttcccgg
    56101 caagtagata tcctgaagcg cggcgtggcc ggccaggggc gcgaggtttg tgatcgaatt
    56161 gttggccagc gtcagatcgc gaagctgggg gaaccggccc gagaggaagc cgatatcgct
    56221 caggttgttg ccgccgagac ggagaagctc cagcttctcc agaccgtaga gcggcgagag
    56281 gtcggcgacc gacacatagt cgaggctgag ttgcgtcagg tttccgagtc cggccagcgg
    56341 cgccagagtc gccaaagggt tttgcgagag cgcgagatcc cgcagaccgg tcagcccggc
    56401 cagcgggctg acatcgctga tgtcgttgcc gactgcgttc agcgagacca gcgccggcag
    56461 tccggccacc ggcgacaagt ccgtgatgtt gttgccgagg aggtggagaa aggcgagcga
    56521 ggtgaaatat tccacccccg tgatgtcggc gatgtgctgc tcattggcgt tcaattcgcc
    56581 gatcgtgtcg acgtcggaag agtgaatgtc cccggtcggc ttatggatat gctcgcggac
    56641 gacccgctcg aaggccgtat cctcgaaagc gacgacaagc tcggccggaa tgtgcacgcg
    56701 gcagcaattc gataggccgc tcgcgttgcc ggcctcatcg atcgtcttca tcgcgaagta
    56761 gtagatgctg tcggagagca gatcgtccac caccaccgtc tgcggctgtc cggcgacgcc
    56821 gggagatacg atgtcggcca gcgggactgc ccagacgaaa gtggcgtcgg tgatggtgtc
    56881 ggcggacgcg cgcaggtcgt aggcattcgc aatccccgtc aggccgtcgt cccccggcgc
    56941 ggtccacccg agcacgactg aaccggtggt gaccgtcagc gcccgcaggt cggtgatcgc
    57001 cgccggggcg gtgaggtcgt cctccccgcc gttgtcgccg tcatcggtgg cgttcttcga
    57061 acaggccagg gccaggcaga ccaacccgat gaccgccggc cacagtactc tcatgcgctt
    57121 catgtccgac ctcctgaatc aaaacctgcg cgccctcgga ccggccggcc ggttgagtca
    57181 accgccgggg atcctcgtgg accatggcgg taatgtaccg cgccgtccct gacagtgtca
    57241 acggcgaact gggggctcga tcgcgcgcct cacagcgaca gccgctggaa ggccatcagc
    57301 ttgttcgcat actcgacgtt ctgcgccacc acagggatgc cggtagcgcg cttgacgctg
    57361 ccgggcccgg cgttgtacgc gacgccggcg agcaggagaa cgtgggcttc gtcggagagg
    57421 ttgagatccc gcttcacgcc gcgggcgagc atggcgaggt agcgcacgac caggtcgtcg
    57481 gccaccgccg ggtcgaaccg ggcgtcgtag gcccggcgcg cctccggcgt gagggcgaac
    57541 atgcgccgcc ggaagctctc catcgtggtg gcgtggagca gatcgtagtc ctctctgagg
    57601 gtctccgcct cggcccgctg ggcccgcgcc cccgcgtcgt cgccttcctg aaaagccgcc
    57661 accgcctcgc tcatctttcg ctggaacgct ccgcggagcc gcaagacctc gtcctgatcg
    57721 gcgaacacgt cggcttcggg gagcgcggcg cccaactctc gcgcggtggt gtgcatgagt
    57781 tgcgccgcac ccgccgcaaa ggaggacgag atagcgtcgt cggcaaagcc cgattcgatg
    57841 gccagcacct tggcggccag caccggcgga atcgggtaaa tccccgcgtg tctggcacag
    57901 gccgcccgga ggcggggaag atgttcgcgc gcctgcggca gctcccggag gatcttctgc
    57961 aggtcccggt cgttatccag cgcgcccgcc gtcgaatcgg cgctcgccga gtcctgcggg
    58021 accagcgccc caagcgtctc cggaagcagc agcgcggtcg cgcaggcagc aaggaacatc
    58081 cgacggtcgg ttgtcaaggt catgatctcc gttctcttcc gccgccgagt cagcctcggc
    58141 ggccgccgtt cagtgtggct gcgttcttcc gatgttacgt ccgggacggc aattgtcgcc
    58201 ataaaacaga ccggcgccgg atttttgtga cagtttttgt cgccgcgcgg ccggattttg
    58261 cgtataatcg gtcggcggac acatgggcgc tgggaaaacc gtcgcccgct cgcggctgtc
    58321 ggaggatctc gtgagagtat tcaaatcgct cgttatctgg tcgtttggag caatgctcct
    58381 ggcgccgttc gtttcgcccc gggccgcccg gcccgagccg gttccctccg acaccgcggc
    58441 ccgcctggaa cctcggtcgg tgaccagcgg gcttttcctg gccgccgtat ttccgggccc
    58501 cttcatacac ggcgccggca actattacgc gggcgccccg gcggccggcg cggtcatgac
    58561 ggtcgcggaa atcgtcggcg ccggggcgat catagccggc ttcgcctcgg gcggcctgga
    58621 cttgttcgat aacgaggagg acgatgagac cggcgaggcg ctcatggccg gcggcctcgt
    58681 gcttttctgg ggctcctggc ttgccgacat cgccaccacc cccttccagg tgcaccgcta
    58741 caaccgccgc cttgcccagc gcggaatggt gttcggggtt tacgcggcgc ctcccgcaca
    58801 gggccgcccg cccgctttcg gggtcacaat ccgattctga gtaagcgccc tgcttcagga
    58861 atgcggaact ctccccggtg ggagcgtcac cgcccgagga tgcgggcggg aagcgtcagc
    58921 accgcccgga gcagctccag cacgccgtcg accgccgccc cgaccacgcg gaacggcagc
    58981 aggaggagcc agacgatcgg gtagagcacg agcgcgagga gcgccagcgg ccacgccaca
    59041 gccagcagaa tcaaccacag aaggaaagtc gccatatcat cgcctccgaa caccggtacg
    59101 cgccgggccg gagaaagatt cgcccccctc tcaccgggtt tcgacaaaag cgtcgatttc
    59161 ggtccagatc cgtccgatca cgtgctcggc cgcttgctca accggaagcg acatctcggt
    59221 ccccggtgcg caggtatcgg cctcgatacc gaaaacgata agctgggggg gcatctgacc
    59281 catggccgcg gcgagcgcca gggcgtcgac cacgctgaag gcgtgcgtgg agtagcggca
    59341 gaacaaatcc tcggggatcg gctcccggag ggcgtcgaag cggtgcaccg tgccggcctc
    59401 gcggccggag acggcgcaat cgacgaggat cacgaaatca gcccggttcc acaagtcgag
    59461 gagatcaagc gcgtcggcca ccccgatcat gacccccact ccgggccgtc cggccgcctc
    59521 gacccgccgg gcgacataca ggccaacccg gtcatccgat cgaaagtcgt tgcccagacc
    59581 gatcaccagg atgcggggcg cggcggccat acctcctcac tcgcgaatga tgcgcagctt
    59641 gaggaagtgg caggagcacg aaatgcacgg gtcgtagttg cgcaccacct gctcgcactg
    59701 ccaggtgaga tcctcctgcg acaggtggag gttgcgttcg acgaagtgca ggaggtcgtc
    59761 ttccacggcc ttctggttct gcgaggtggg ggggacgatg cgcgccgaga ggatcagccc
    59821 gtggccgttg agcgtgtact ggtgcaggag catgccgcgc ggcgcctcgg tcgcccaggc
    59881 ggccgtgccc gcccgcgggg ggaccggaat cgccggctgc gccggcggct ggtagcgctc
    59941 gatgaggcgg agggcctccc agcaggcgaa gacgagctcg acgctgcgga cgatgatgct
    60001 cttgaagggg ttgcgacagg tcgggccgag cccggcctcc cgggccgccc gctgcgcgat
    60061 ctccggcagt tggccgtaat tcaggctgta ccgcgccagc ggtcccacga ggtagtggcc
    60121 gcgctcgatc acccgcgcgt gcagcgaggt cgagtgcttg acgtgctcct cttcgaagta
    60181 cttgaagaaa tcgttgaagg ggatgtcgat accccggttc gagacgatcc gcccctcggt
    60241 gatggcgtac tcctcggggt gccggatggc caccatctcg tagtccggct caaaatccgg
    60301 gaacgacaac ccggccgtcc acttgaccgt ctccagcgag gcttccagcg cccactcgag
    60361 atccttcttg agcgcgagca cggccgcccg cgagggcacg ctgtagaacc caccggtgcg
    60421 gatattgatg gggtggattt cccgcccgcc cagcagcacc atgacgtcgt tgccgatctt
    60481 cttcaggcgc agcccgcgct tgacgatgtc ggggtagtcg gcggccatgt ggatgccgct
    60541 cgcgtagcca aggaagtcgg gggcgtgcag caggtagacg tggagagcgt gcgactcgat
    60601 ccactcgccg aggaacaaga ggcggcgcag atcgcgcagc ggaccctcga cggccgcccc
    60661 gcagatcgcc tccatcgcct gggccgagct catctggtag gccaccgggc agatgccgca
    60721 gatgcgggcg gtgatgtccg gcgcctcgga gaaccgccgg ttgcggagga aggcttcgaa
    60781 gaaccgcggg ggctcgaaga tgttgaagcg gacctcggtc accttgtcct gcttgacccg
    60841 gaccgtcagc ccgccctccc cctcgacccg cgcgagcatg tccaccgtga tcgttctatt
    60901 tttcatgcgc ttcgctctct ttccggaatg gctccgcgta cgcgttgtac tggcggaacg
    60961 tgcggactat gtcgtcgctc gacatctgct gctccttgaa ccagttcgcc agcgacgacg
    61021 gattgggggt ctccttcggt ccgaaacagc cgaagcagcc gcggttgtag ctcgggcaga
    61081 gggcgtcgca cccggcctgg gtgaccgggc cgaggcaggc gagcccttgg gagaccatga
    61141 cgcagacggt cccgcgccgc ttgcactcga ggcagacgga gtaggggggg acgttgggct
    61201 tgcggccgtt gaggagggca ttgacgacct cgaggagctg gtacttgctg atcgggcagc
    61261 cgcggagctc gaaatcgacg tggacgtggt cggcgatcgc ggtcgacttg cgcagcgttt
    61321 cgatatactc cgggcgcgcg tagacggccg ggatgtaggt gcggatttcg gcgaaattgc
    61381 ggagcgcctg gatcccgccg gaggtcgcgc aggcgccgat ggcaatgagc agtttcgatg
    61441 cccggcgcac cttgtggatc cgttcggcgt cgtggggcgt cgtgatcgac ccctcgacca
    61501 gcgatacatc gtagggcccg gggaccactt cgcgcgaggc ctcgaggaag ttggcgatct
    61561 ccaccgcccc ggttatcgcc agcagttcat cctcgagttc cagcagactc agctggcagc
    61621 cgtcgcacga ggcgaacttc cacactgcga gtttcggttt gactgacttc gccataatgt
    61681 catatctccc gctgcgtaaa gagcggtttt acgcggtcca ggcggaacac cggcccgtcc
    61741 ttgcacacga acagcgaccc cgtctggcag tggccgcaca ggccgattcc gcatttcatg
    61801 ttgcgctcca tcgacacgta gatctgctcg tcagccagcc cgcgcttgga cagcgccagc
    61861 gccgtgaacc gcatcatcac ctccggcccg cacgccatcg ccaggcagtt ggccgcgtcg
    61921 aacgtcacct tcggaaccag cgtcgtcacc acgccgacat tccccgtcca ctgccccatg
    61981 ccgcggtcaa ccgtggtcag cacctcgatc tcgggccggg ctccccactg cgcgagcatt
    62041 ttcggataga gcacgtcgtc cggcgtccgc gcgccgtaca gcaggatcac cttgccgtac
    62101 cgcttgcggt gcttgaggag atggtagagc gtggggcgca gcggcgccag tccgatcccg
    62161 cccgccacca ccaccacgtc gcgccccttc gcctcctcga ccggccaggc ggtgccgtag
    62221 ggaccgcgca ccccgatcac gtcgcccttg cgcagcttct gcatctgttt cgtgaccgac
    62281 ccgaccgcgc gcgtcgtgtg aatgagggcc cggggtttgg ccggatcgcc ggatatcgat
    62341 atcgggactt ctcccacccc gtagacatag agcatgttga actgaccggg gaggaaggcg
    62401 aacccgttcc cctcctcggt cggcgccagc tccagcgtaa aggtatcgta ggtctccttg
    62461 accacccgcc cgatgcgata gggctgcccg agcatgggat cgaggagggc cggagactcg
    62521 gctgcggcat ttgtcttagt gatggccata tacatccagg agctgcatgc gcgtggcgtc
    62581 gagccgttcg gcgatgacgg tgacgaaccg gcgcatgact tcgtatccca gttcatggtc
    62641 gctggcgaac ctctcgcgca ggcacttgcc gtcgaacacg atcgcccgcg ttgtttcgac
    62701 cgcccgcgcg tcaaaatgcc accggtaggg aggcaccagc caggaccacc ccagcacctc
    62761 gcccgcctcc cggctctgaa tggctaccgg tccgcggtgc ggcacgtgcg tctccaccag
    62821 cactttgccc tggcggatca cgtagaactt gtccgccgct tcgccctccc ggaacagaaa
    62881 ctcccctttc gggaacaccg tgttggcggc acacccgcag ataatcgcgt tgtgcgcagg
    62941 cgagaggtct ttgaagaagg agtgctccga cagaattttc tcaagggtct ccataggttc
    63001 ctcctgactc aggtatgcgc ttccgcgcgc tggaggctgt gtgcgaatac tcgggcttct
    63061 tccgttatat ctatgcccac cgggcaccag gtgatacagc ggccgcagcc gacgcatccg
    63121 aaggtgccga actgatcgac ccacgaggcc agcttgtgcg tcatccactg ccggtaccgg
    63181 gagtagggcg agggccggat gctcccgccg aagatgtacg aaaaatcgac ggtgaaacag
    63241 ctgtcccact tgcgcacccg ctgggccttc tcccccccga gagcggtcca gtcctcgacc
    63301 gtcgtgcaga aacaggtcgg gcagaccatg gtgcagttgc cgcaggtgag gcagcgcgcg
    63361 gcgatctccg cccagtgcgg gtgttcgacc gccttttcca tgcgggcttt cagaccgtcc
    63421 gtgtccaact gccttcccat gtggtgggcg gcatcggccg tcgcccgcgc cgcctcctgc
    63481 cggtcgctct cgcccgccgg gggcgccgcc agttcggcga gcaccgcctc tccctcgacc
    63541 gtaccggcct cggcgatgaa gacgtgccgg ccattcgccg acagctccgt cagcacgatg
    63601 tcgtagccgc tgcgggcctc cgggccggtc cccatcgagc cgcagaaaca ggtcccgccg
    63661 ggccgggtgc agttgacggc gacgagaaac aggttctcgc gctggcgccg gtagagcggg
    63721 tcggcgtacg gtcccgcggt gaacactttg tcctggatcg cgatggcggc cagctcgcat
    63781 ggccggacgc cgatgaacgc ccgccggggc ggcggctccg ccgccggcgc ggtccggaaa
    63841 ccctccgcgc tgcgctccgc ctcccagagc gtcgccgtcg gcaggtgcag atacttcttc
    63901 cacgacccct ggccgacgac gtaaccgaag accgcggccg ttcccgtgtg cgtgagccga
    63961 taggttccgc cctcctgatg gtcggtccat ccgagcggca ggtcctcgac ccgctgaatg
    64021 tcatcgtaca cgatgccgcc atttctgacg gtcgggccca gcaagcggtg accgcgccgg
    64081 gcgagcgcat cgaaaagagc ctggagatga ctccgctcaa tgacgcgcgg ctgataggta
    64141 tggtgtgtct gtgtcgacat tgtgattttt ttcacatata ctccgaccgt aatctacatc
    64201 cgcttccccg ggtgtcaagc gaaaagtgcg cccccgtccc gaaaatcggc gcgcccggcg
    64261 gacggggcgg tcccgatcgg tcttgacaaa ccgcgccccg catggtatac aaactagtta
    64321 aacgtccaca tattcttcga gggtgtgtga accccgtccc gcggcgctcg ctctggggtc
    64381 gccccgaagt gccgtcagac gacacacgtg agcgatggcg aaggcccctg gcctgcgatg
    64441 gcggattact gcagccgtgc tcctggcggc cctgttgccc ctggcgttgg ccggctttgg
    64501 cgcctggatc gttttcggca gcctctttga gcagcgttct ctggagtcgc tccgcgggct
    64561 cgttcgggcg cacgcccgca ctatagacgc cgacctttcc gagcacatcc gtctcctgca
    64621 ggtgctggcg gcctcgcaga cgcgcacgcg tttggccgac gatgcgcaac tcgacaccct
    64681 tctggccgaa ctcaaccgcg ccggcgacaa cagcttcacc gacctgggag tcatcgacgc
    64741 cgagggggac cacctcgcct atgccggtcc cttcgacctc aagggccgca actatcgcgg
    64801 ggacgagtgg ttcggcgagg ccatggagcg cggcgtttcc ctcagcgatg ttttcctcgg
    64861 atttcgcggc gcgccccact gcgtgattgc ggtacgggcc gggaatgccg ccgagggttt
    64921 ctggattctc cgcggcacga tcaactcggc gcggttcgac acgttggtgc aggcgggcaa
    64981 gtgcaacggc ggcgacgcct acatcgtcag ccgccgcggg ctctaccaga ctgcgtcgcg
    65041 gcgcggcgcc cttctggaca cggcgcgcgg catcccgctt gttccccacg agggcgcggc
    65101 ggactaccgg gccaccctgg ccgggaccga cctcatcctg ctcaccgcct ggctccagtc
    65161 ggggcgctgg ctgctggtgg tcgagcagga ccgcacggcg ctgcgcgccc cggtcaacca
    65221 ggcgatcacc cggggggcgc aggttgtgct catcgccgtc ctcatcctca tcgtcacgac
    65281 cgtcatcgcc acccgccacc tctgccgtca gatcgaccgc gccaatgcgc agcgcgagga
    65341 gctgtcgcgc gcgtttgtcc gctcggcccg cctagcctcg atcggggagc tggccaccgg
    65401 cctcgcccac gaaatcaaca acccgctggc ggtgatcagc gccgagcaca ccaatctcgc
    65461 cgacctgctc cgcgcgagtg acctgccgga accgacccgc ggcgacctgc tcgagtcggt
    65521 cgagcgctgc cgccgccagg tgcagcgctg cgccggcatc acgcgcaaga tgctccagtt
    65581 cggccgccgc cgcgaagccg cgcccgaacc gaccgacatc accccgcgtc tccaggagat
    65641 catcgccctg ctccgccgtc gcgcgggtgt tcgcaacgtc gctctccatc tcgagacggc
    65701 ggccgggctc cggccggtgc tggttgaccc catcgagttc gaacaggtca tcgtcaacct
    65761 catcaataac gcggttgacg cgctgcccgg cggcggcgac gtctgggttc gcgccggcgc
    65821 cgccggcgac cgcctccgca tcgaggtcgc cgacaacggc gtcggcatcc cggccgacac
    65881 gctcgaacgc attttcgaac ccttctacac caccaaaccg gtcggccagg ggaccggcct
    65941 cgggctgtcc gtgtgctacg gcctggtcgg cgagtggggc gggcgtatca ccgccgagag
    66001 cgccgtcggc cgcggcacca ccatgcgggt ggagctgccg ctcccgcacc ccgacaattc
    66061 caccgcgaga ccatagaggg atccacatgc acaacccgta tcagatgatc cgcgtgcttt
    66121 tggtcgacga tgagcgggat ttcctcgcgt cgaccgccca ggcgctgacc cgccgcggct
    66181 tccgggtcac caccgccgac agcggcacgg ccgcgctccg cctgctggag ggggacttct
    66241 tcgacgccgt cgtgctcgac gtgaagatgc ccgacgttga cggcctggag gtcttccggc
    66301 aggtccacgg cctgcggccg cagctcccgg tcattctcct caccggctac gcctcggccg
    66361 cgcaggcggc ggccgccgcc gaatccggcg tcgccgtcta cctcgccaaa ccgtgcgaga
    66421 tcggcgacct ggtggagcat atccggaacg ccctcggcgc gcgccgggtg gtcgatgaaa
    66481 acggtttcga gtccgccgcc atggaggaag tcagcgtgct cctggtcgac gacgaggacg
    66541 agctcatcgc cgcgctttcg ccggtgctgc gccggcgggg catgacggtc agcgcggcct
    66601 ccggcggcga ggaggcgctc cgcgttctgc gcgagtcgtt cgtcgacgtc gtcgtgctcg
    66661 atctcaagat gccgggcatg gacggcctcg cggcgctccg ccacatgaag cgggagttcc
    66721 ccgacgtcga gattgtcatc atgaccggcc acccctcggc ggattcggcg gtccagacga
    66781 tcaaggcggg cgcgagcgag tacctcatga aaccgccgga caccgatgaa ctcgtggggg
    66841 cgatccgcaa agtcttccgc cggcgccgcc agcgcctcga acaaaaacag cagcgcctca
    66901 tcgacgaggt ccggaagcgc tatcccgagt gaccgccggc gttgcggatt cggcggcccc
    66961 gtctctctca cgaaaaaaaa tgccgccggc agggtagttt ggccgacggc actgtggagg
    67021 aaccaagtac acaaatagag cgcgagccct atttcctgat gtaacggttc ttgggtgcaa
    67081 tgaacaggcg ccggccgtgg agcgtgccgg cgtccgttcc ctggtttatc tctctctcgc
    67141 tggtctcctt ccttcgcttg gtctccggtc ttccgtttaa cgtattcgcg tttcgatagt
    67201 cgcttctttc gctaccggtt gtcggtcagc cgccgcaggt acgcgggcac ggtccggtcg
    67261 gtgtcggcaa acatcggcac gcgcggtttc cccttgccgt tgccgttgcc gttgccgttg
    67321 ccgccgcggt cgccgtactc ggggacgccg gccgcggcca gcacttcttc cggctcggcc
    67381 gcgggcgccg gttccggggg ccgcgcgacg gcgctctcgg gaaagagcga gatcggcgcg
    67441 cccgagggga cttcgcgcgc cgcccgctcc cgctcgtcct tctcgacctg ggcgcgcttg
    67501 gtctcgacac cgccgaaccc ggtggcgatg acggtcaccc gcatctcgtc ggccatcgtc
    67561 gggtcgatca ccgcgccgaa gatgatgttg gcgttgttcc ccgccgcctc gtagatgagc
    67621 gaggtcgcgt tgttgacgtc gaaaagagtc atgtcctcgc cgccggtaac attgatgagg
    67681 acgccgcggg cgccgctgat cgagacgttt tccaggagcg gcgacgagat cgcctcctgg
    67741 gccgcatcca ccgctttctc ctcccccttc ccccagcccg tgcccatgag ggcgtcgccg
    67801 cgctcgagca tgaccgtgcg cacgtcggca aaatcgcagt tgatcaggcc ggggatcgtg
    67861 atgaggtcgg aaatcccctt ggtcgcctgg tgcagcacct cgtccgccac cttgaaggcg
    67921 tccgcgaggg tggtcttgcg gtcgacaatc tgcaggagcc gctcgttggg gatggtgatg
    67981 agcgtatcga ccttcgagcg cagctcttcc aggccggtct cggcccggtt gatccggtgc
    68041 gccccttcga agcggaacgg cttggtgacg atggccaccg taagcgcccc ggcttccttg
    68101 gccagctcgg ccactaccgg cgccgccccg gtgccggtgc cgccgcccat gccggcggtg
    68161 atgaagacga ggttggggcg gccgatcgct tcggccacct gggcacggct ctcgtcgatc
    68221 gcccgcttgc ccacgtcggg attggccccg gcgccgagac cgcccgtgag ctcgatcccg
    68281 atctgcacct tcttgtccgc ccggttcgca tcgagcacct gggcgtccgt gttgatggca
    68341 atgaattcta cgccctgcag gcgggcctcg atcatccggt tgatcgcgtt cccgcctgcg
    68401 ccgcccacac cgactacttt gatgttcgcg tagccgatga aatcctcggc gaaattgaaa
    68461 ctgtgtttac cctcagccat cggttcctcc atgaggctgt tgtgttagaa cttattggaa
    68521 atccatttct cgaatttctt cagcaaaccc cgcactttcc cccgggccgt gccggccagt
    68581 tccggttcgt tgtgaaagcc gtagaggagc aggccgtgcg cggtggcgaa acaggcgttg
    68641 ttcatctcgt cgggaatgct cgtaaaaccg cggatctgcc ccggccggac agggatgtcg
    68701 aggagctgct cggccagcgc gatcgtcccc ggcagcagcg acccgccccc cgtcaacacc
    68761 accccgccgg cgagcatgtc gtcgacgtcc gcgcggcgca gctcgcggat caccagcgcg
    68821 aggatctcct ccatccgggg ctcgatgatc gaggcgagca cgttgcgcga gatttccttg
    68881 gcctcgtgcc cggcggcgct ctcgaccggg atcatctctt ccgggtcgac ctgcgaggcg
    68941 agcgccgcgc cgtgcgcgat cttgagcgac tccgcctgct ccaccgtcgt gcgcaggccg
    69001 atcgcgatat cgttcgtcac gttccgccct ccgagcggca ccaccgccgt gtgccggatc
    69061 gccccgtcgt ggaagatcga gatgttcgtc agctcccccc cgaggtcgac caccaccacc
    69121 ccgtgctcgg cctcgtcgtc ggtcaggatc gcttcggaca gggccagcga ttcgagcacg
    69181 atatggtcgg cgtcgagccc gcaccgttcg agcgcccggt agatattgcg cgcggtcgtc
    69241 accgaggccg tcacgatgtg ggcctcgacc tccagccgca cgcccgacat gccgatgggg
    69301 tccttgatcc ccgtctggtc gtcgaccgaa tactcctgcg ggatgacgtg gatgatttcg
    69361 cggtcgaccg ggatggcgat cccgcgggct gcgtcgaccg ccttgcgcac gtcggccgcg
    69421 gtgatctcgt tgtcggcccg ggtcacggtc accacgccgt gcgagttgat cgaccggatg
    69481 tgttcgccgg cgatcccgac cgtcacccgg tcgatcacgg tgccggagac gagcgcggcg
    69541 tcatccacgg ccttgcggac cgaccgcacg gtccgctcca tgtcgacgag gacgccgcgc
    69601 cggagcccct cggccggggc ttggccgtgg ccgatgacgt agattcgccc gtcggtgtcc
    69661 atctcggcga ccaggcagac caccttggtg gtcccgatgt cgagggcggc tatgatgttc
    69721 tcggtcgcca tcgccggtca gtctttcgcc tcctgggtcg cggcctcggc cttggcccgc
    69781 tcgcgggccg cttccgcccg ccgctccatc tcctcgtcgg agagtgcgca gatgatcatg
    69841 ttttcgtagc gcaggtccat gcggatcacg ccggagaggt cggcatcgta gcggcccgtg
    69901 aattcgacga accggtcgaa atcctcaagc acgtggtcgg gccgcacccg caggcggtag
    69961 gggagtccgg cgaacgtcac ctccaggtac tcgcgttcgc cgaaatcgat ctcgtccaac
    70021 aggcggtaga ggtcggcgcg cgattcgcgc aggtcctcga gctggtcgat caccggcagc
    70081 acccgcgggt cggcgcagat cgagtggagc gcccccgtcg ttaggccggt gaacaccggc
    70141 tgctcccagt tgatgtccgg ggcgtcgagc gcaatgaggc gggcccgccg gttcagcccg
    70201 aacagcttgc ccgtctccat ccccaccacg aaacagacgg gccggaactc gttggtccgg
    70261 acatcgatcc cccccggcaa ttcgaaccgc acgtcgactt tgtagacgtc ctcgtcggcc
    70321 agcagcttct tcgccagccg ctcgaccggc tgggcggcga gagagccggg gcggagcatc
    70381 gcaaactgct gccgccagtt ctcgaccggc ttgccgtcga ccgtcaccgc gcgcaggcgg
    70441 caggcatcgg tgaaattgac cgtcaccgtg agccccaggc cgaccaggag gccggcgagg
    70501 gcgaacaacc ggtattttct gccgcttatc cccaccgctc cctcagcgcc tccagaatgt
    70561 tcttccgctc cagcgtgatg gagccggccc ccatggtgat gacgatatct cccggctgcg
    70621 cccgctggac cacttcccgc gccgcgttct gccgctcgcc gatgcaggtg aattggccga
    70681 gaccgcgcgc ggcggctttc tcccggatca gatcggccgt cactcccggc atcggcttct
    70741 cccgcgccgg gtagatgtcg acgagcaggc actggtcggc gatggccagc gcctgcgcga
    70801 attcatcggc gaaatcccgc gtccggctga acaggtgcgg ctggaagacg acgatcacgc
    70861 gccgcccgta cacgtcccgc gccgtgcgca gggtcgcttc cacctcggtc gggtggtggg
    70921 cgtagtcgtc gaccaccgtc acgccgccgg cctcgccgac gatctcgaag cggcgcccga
    70981 cgccggtgaa tccgcgcaga ccgtcggcga tcagctcgaa cggcacaccc agttcggtgc
    71041 aggcggccgc cgccgcgaga gcgttggcga cgttgtggcg ccccggcacc cgcaggagga
    71101 tgtcgccgag gtactgcccg cgccggtaga gcgagaagac cgaccgtccc ggctcgagcc
    71161 gcacctccac cgcgcggtag tccgcctcga cggaaaagcc gaaggtcacg taggggcgcg
    71221 cgaggcggtc gcgcatctgc atgagctgcg cgttgtccgc cgacagcacg accgagccga
    71281 agaagggcac gcggttcata taggtgagga aggcgtgccc gaggtcatcc aggccgcggt
    71341 agcagtcgag gtggtccgcc tcgaggttgg tcaccaccgc catggtcggg aacatcgcga
    71401 gaaaggaccg gtcgaattcg tcggcctcgg ccaccaggta ctcgccttcg ccgagcgacg
    71461 ccccggtgcc gagctcggcg acgattccgc cgacgatgat ggtcggcttg agcccgccgg
    71521 actggaggat gcgcccgatc atcgaggtcg tcgtcgtctt gccgtgggtg cccgccaccc
    71581 cgatggagta cttcaggcgc atcagttcgc ccagcatttc ggcccgcttg atcacgggga
    71641 tgccgcggcg gcgcgcctcg accacctccg ggttggcctc gccgacggcg gacgaaatca
    71701 ccaccacctc ggcatcgccg aggtgcgccg cgtcgtgccc ctcatggatg cgcaggccgc
    71761 gttcggccaa atagcgggtg aggtccgagg ccgtctggtc ggagccgctg atttcgaagc
    71821 cgaggttgtg gaggatctcg gcgatccccg acatgccgga gccgccgatc ccgacgaaat
    71881 acagcttgcg gatttttcca aacatgagtc tgtcgttcgc aaagggtgcg atttctttgg
    71941 gtttggtggt gactgccgtt ttcacgcccg cgcctttctc tcacggttga tgaggtcgat
    72001 gatgtcctcg gcgatgatgt ccgtcgccgg ccgtccctga cgccgcgcgg actccagttt
    72061 ctctctcatg gtccggtact cggccgcctc cagcaggtcg gccgcctcgg cgatcaaatc
    72121 gtgccgggga agatcgtttt cgtcgatcac ccgggcgaga cccgcggcgg cgaggtcgcg
    72181 ggcgttcttg cgctggtggt ctccggccgc gtggggatag gggatgagaa tggccgggat
    72241 ggcgcaggcg gccagctcgg ccagcgtgag cgccccggcc cgcgcgacgg ccaggtcggc
    72301 cgcggcatag accgcggcca tgtcatgggc aaacggaaag agggcgcagc gcgctgccgt
    72361 gccgcctgcc ttcgcagaaa cctccgtgta atccctcttt ccggtctgcc acagaatctg
    72421 gcagccgtcc gccggcgcgc cgccggcgag gctccggagc accgcctcgt tgatcgcccg
    72481 cgctccctgg ctgccgccca gcacgaggat cgttttcttg gccgggtcga gcccgaaggc
    72541 cgccgccgcc gcggcgcggt cgccgccgct cagcccccgc cgcaccgggt tcccggtcgt
    72601 gaggacctcc gcctccgtgc ggaggtactg ccgcgcgccg tcgaacccca ggtagatctt
    72661 gcgcgcgtgt ttcgccccct ggcgcgtggt gatgccgggg aaggaattct gttcctggag
    72721 cacggtgggt acgccgcgcc agcgggcagc tttcaacacc ggcagggcga catagccgcc
    72781 cgtccccacc accgcatccg gccggaagcg cccgagcagg agccaggccc ggacgagcgc
    72841 ggtgacgagc acgaacggca ccagcaggtt ggccggcgac agcgcgcgca ccagtccacg
    72901 gacgttgata acgtggaggg gatagccgag ggcgtcgcgc atccggtact ccaggccgcg
    72961 ccgcgtgccg acaaagagaa tctccaccgg ggccgtcccc gcaagcagct cgcggacgcg
    73021 gtcagcgatc gcaatcgccg ggaagaggtg ccctcccgtc ccgccgcccg cgaacaccag
    73081 ccggtactcc tgcgtccgcc ccgtcattgc ttcgccctcc ccaccatccg ccgcgacagg
    73141 ttgagcagga cgccgatagt tatgctcgtg atgaggagcg atgagccgcc gtaggaaatg
    73201 aagggcagcg gcaacccggt gaccggcatg aggctcgtca ccaccgcgac attgaccgcg
    73261 atattgatga acagcgacag caccacgccg ctcgccagca ggaacccgaa ccggtccggc
    73321 tggtcggccg caatcttgaa gccccggtag agaatgaacc ccaaaagcga cagcagcagg
    73381 agcagcccca cgagcccgat ctcctcgccg gcggcggcga agatgaagtc ggtgtgcggg
    73441 tagggcagga agaagtgttt ctgccgcccg tcgccgagcc cgacgccgaa aatcccgccc
    73501 gcccccagcg tgagcgccgc ctgcttcgac tggtagctcc ccgccagcgg atccatgacc
    73561 gaggcgagga agtccatcac gcgggccttt ttgtacccga tcacgaacac cagcagggcc
    73621 gccccgccca tcgccggcag agccgcgtag gcgatgtgct tgatcttcgc ccccgccaag
    73681 aagaacagcc ccaccgccgt cgcgaatatc accatcgaca tcccgagatc cggctccagc
    73741 acgatcagca ccagccccgc cccgagcatc ggcccgtagg gcatccacac ctgcttgagc
    73801 ttcgtcacgt cgcggttgcg gttggccagg gagaaggcga ggaagtagat catgacgaac
    73861 ttgaacagct cggcgggctg gagggtgagg ggtccgagga agagccagcg atgcgagccg
    73921 ttccgcgccg gcatgagaaa gaccagcgcc agcagcccga gggtgagcac gaacagcagc
    73981 cacgaccaca ccgcccaccg ctggagatcg atgcggcaga ccagccagat cgcggcgagc
    74041 gaaataatcg cccagacgaa ctgccgcccg ataaagtgca ggtgcgagcc gaaccggctc
    74101 tccgccagca tcgacgaggt cgagtagacg agcaccattc ccgccaccac cgcgacgagg
    74161 taggcgatca gcagcttctc atcgtagccg cccttaagga gcaaccgggt tgtggttttt
    74221 cttaccattt ttcaattgtg cgaccgcctc cttgaaggcc cggccgcggt gttcatagtt
    74281 gtcgaacatg tcgaaactgg cgcaggcggg cgagagcagg acggtctccc cggggcgcgc
    74341 ctgcgcaaaa cacttctgca ccgcctcctc gagagtgtcg gcgaactgca ccgggaattc
    74401 ccggcccagc gcccggaaga tcttctcccg ggcctccccg atcgccacca cgccgcgcac
    74461 ctttccccgg gccagcggcg cgagcggctc atagggcgcg cccttgtcgc gcccgccgag
    74521 aatcaggtag accggcgtgt ccaccgactg cagcgcgaaa cagaccgaat ccacgttcgt
    74581 cgccttggag tcgttgatga aatgcacgcc cgcgacgctc ggcacccgct cgagccggtg
    74641 ctcgacgccc gggaacgtgc gcaggacccc ggcgagtacg gcggcctcga cgccgaacac
    74701 ggtcgccatc accgtcgccg ccgccgcgtt ctgcaggttg tggggccccg ggatgaggat
    74761 atcgcgggcc gggatgaccg gctcctcggc ccggtctttg cggcgccaga gcacccccgc
    74821 gcgcacgaac gcgcccgcct cggtcgcgtc ccccgtcgtg aaatacaccc gcgccgcccg
    74881 cgtggcgatc cgctcgccgg acgtcaccgg atcgtcgaga ttcgtcacca gcacgtcgtc
    74941 gggaccctgg ttctcggtga tccggtactt cgtccgcttg tactcctcca tcgtcccgtg
    75001 ccggtcgagg tggtccgcgg tgatattgag aatcgcggcc acggccgggc ggaaatcggc
    75061 gatcgtctcg agctggaagt tcgacacctc cacgaccgcc accccgtcgg gcggcacgct
    75121 cgaggccgct tcggcgaagg gccggccgat gttgccgcaa atgcaggtcg ggcggcccgc
    75181 cgccgcgaac atctcccccg ccagcgtcgt cgtggtcgtc ttgccgttgg atccggtgat
    75241 cgcgacgata cgcccgcggc agaaccacga ggcgaattcg atctccgaga agagcggaat
    75301 cccccgctcg cggatccgcc gggcgatctc cgcggagagc gggatgccgg gggagatcac
    75361 ggcgtagtcg ctctgcagga tccggtcgct gtggccgtcg gtctcgaacg gcacgcccga
    75421 cttctcaagc tgggcgcagg cgggcgcgag cgcctcgcgc cggccgctgt cggtgacgaa
    75481 ggggatgccg cccatttctt tggccagcag cgccgccgcc agcccggacc gggccatccc
    75541 gatcaccccg accttgcgtc cgcgtatcct ttcttcccgc gtcattgtcc taccgcactt
    75601 tcagggtcgc cagcgtggcc agcgtgcaga gcgccccgat aatccagaac cgcaccacca
    75661 ccttctcctc cggccaaccg agcagctcga aatggtggtg gatcggcgcc atcttgaaca
    75721 cccgcttgcc tccccggtag cggtatgaga gcacctggat gatcaccgac agcacttcga
    75781 cgacgaacac gccgccgaca atcaccagga gcagttcttt cttgagcatg atggcgatcg
    75841 cgccgagcgc gccgccgaga gcgagcgacc cggtgtcccc catgaagacc tcggccgggt
    75901 gcgagttgaa ccacaggaac ccgagggccg acccgatcgc cgccccgcag aacacggtca
    75961 gctcgccggc gcccggaagg tactcgatct ggaggtagct cgagaaatcg agccgcccgg
    76021 tgagataggc gatcccgccg aaagcgagga aggccagtcc ggacagcccg atcgccagcc
    76081 cgtccgcccc gtcggtcagg ttcaccgcgt tcgaggcgcc ggtgagcacg aggatcatcc
    76141 acgggatgta caggacgccg aggtcgagca tgtagttttt gaagaacggg atcccggtca
    76201 tcccgtcgta gagaccgttc ggggccaccc acagcaggag gcctccgaac acgagcccga
    76261 gccccacctg ccccatgaac ttcttgcgcg ccaccattcc gttgcgctgg tgcttgaggg
    76321 ccttgctgta gtcgtcgagg tagcccagca gtcccagcca cgccgtcacc agcagcgcca
    76381 tcagcacgta gaaattcgtc agatccgacc agagcagggt ggggataatg atccccgaca
    76441 caatgatgag cccgcccatg gtcggcgttc cggctttctt ctggtgcgac gccggccctt
    76501 ccgcccggat cctctccttc acctggtgct tgcgcaggag cccgatgaag aacggcccga
    76561 ggatgaggca gatgaagatc gccgtcgcca tcgccccggc cgtccgaaac gtgatatacc
    76621 ggaacaggtt gagccccgac acctggtgcg ccagtggata gagcaggtgg tagagcatta
    76681 cagaacctcc tcacccggtt cgcccaggcc gttgagaacc gcttccaggc cgatcccccg
    76741 cgaggccttc aggtacacca ggtcgttttt ctgcacgatg gcggcgatct ctttcgcgca
    76801 ccccgccgcg tcggcacagt gccgcaggcg cccccggtcg gccccggacg cgaccgctcc
    76861 ctcgagcgtc tcgcgcgagc gcggcccgac cagcaccgcc aggtcgaacg ggtagcgcgc
    76921 cagcacggcg ccgctctccc gatggtagcg gggcgattcg gggccgagct ccagcatgtc
    76981 gccgagcacg accacgcgcc gcccggcggt cgggagggcg aagaaccccg ccaggcccgc
    77041 cctgacgttt tcggggttgg cgttgtagca atccacgagc acccgcacgc ccccgcgcgc
    77101 gaccaactgc ccccgcatcg gcgccgacgc caattctatc tgcgccgtct ccacgccgtc
    77161 aaaccggtag cctagcgacc ggaacagggc gtaggcgcag gtcaggttgt acacctggtg
    77221 cttcccgacc agcggcatcc ggaacgtccg cccctcgagc acgaccgtca tcatccccgc
    77281 gtcgtcgatc ccccaggcgt ccggccggaa atccgcggcg cggtcgaggg cgaaggtcac
    77341 gtaatcggac cgcaccttct tcgcctcggc cgcgagcacg gggtcgtcgg cgttgatcac
    77401 cgccggcgcg cccggccgcg ccccccgtac gagctccagc ttggcctgcg ccaccgcctc
    77461 aaccgttccg aggaactcca ggtgggtcgc gctcacgttc gtgatcgcca ccgcatcggg
    77521 ctgcacgatc tccgccaggc ggggcatttc gacgtcggtc gaaatcccca tctccattac
    77581 cgccgcccgg cagtccgcgg gaatccggaa aatagccaac ggcacgccgt agaggttgtt
    77641 gaggttgccc ggcgaccggt aggcgcgcgg ctccaccgcg gcgagaagcc ggtaggtgag
    77701 ttccttggtt gtggttttcc cattcgaccc ggtgatcccg acgaagcgcg ccgcgaccgt
    77761 gtgccggtag tggcgggcca gggcaatcat cgcctcgtgc gtgtgcggga ccgtaatcac
    77821 cggggtgtcg cccgcaagcc gcgccagctc ggggtggtcg aactcggcca gaataccggc
    77881 cgcgccgcgc tcgagcgcct gcgcgacgaa ctcatgacca tcgtgcttct ccccccggat
    77941 ggccacatac agctcgccgg ccgcgagcgt ccgggtgtcg atggagacgc cccggaagga
    78001 ggcggtgcgg tccgcggccc ggtggacgcg tccgccagtg atggcggcca gttgatcgaa
    78061 acgcaacttt atcacttccg tgccgttgaa tttgccccgg ccgatgcctt cttgtatccc
    78121 attgccgcca gcgccgcccg cgcttcctcc acgtcgtcga aggggcggcg ctcgccgttg
    78181 acttcctggt accgctccgc gcccttgccg gccaggagca cgacatcccc cgacgcggcc
    78241 gcgcgcaggg cgagtgcgat cgctttccgg cggtccggtt cgatcgcata ccggtccccc
    78301 cgcaggccgg gccggatgtc ttcgattatc gccagcggcg attcggtccg cgggttgtcg
    78361 ctcgtgacca ccgcgtagtc ggccatggtg gtcgccgccg ttcccataag cggccgcttc
    78421 ccgcggtcgc ggtcgccgcc gcacccgaac accagcagca cccgtccggc cgtcatctcc
    78481 cgtgccgact ggcagagccg ctcgagcgca tccggcgtgt gggcgaaatc gacatatacg
    78541 ccgaagggct
//
LOCUS       DAOPOE010000004        72343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_463_length_72343_cov_5.927056, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000004 DAOPOE010000000
VERSION     DAOPOE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 72343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72343
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_72343_cov_5.927056"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKM94_01135"
     CDS             complement(<1..1062)
                     /locus_tag="PKM94_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD65269.1"
                     /translation="MNQAQIEKTAKIRMTGVGGQVGAGAEHVEPLDVLADLEATLDEV
                     AEANPRLTSEETHTSKSVEDLKALLEVSLAINSSLVTDDVLQIVMRKAIELMQAERGL
                     VMLLDDANQLQVRSAYNLCRDEMMDEDFKVSNSVTSRVAETGKSIYTSDALSDERYAN
                     QRSVVELHLRSIMCVPLKVKEQVVGVIYLDNSSESRMFLKSDLYLFELYAQLVSNALH
                     NANIYDRLLNLQRYNESVIAKAPMGTVVLDSDGHITTINTAALEIFDFNGDKIVLVGA
                     GDKPTRFLDLLQETEVTRWAHMINTALATQEDFSDSRYFHNTGYLEKALSIKISPISQ
                     LPHGKDGVIMAVEDITEKVI"
     gene            complement(1161..1814)
                     /locus_tag="PKM94_01140"
     CDS             complement(1161..1814)
                     /locus_tag="PKM94_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOD65270.1"
                     /translation="MRFFRPQARRRSPAALLALILAAAAPARGADPPGGVEYDIVRAD
                     DSLAVWLNLTPFFGHPEYRSLQDGIDYACEIAIELQRPRKLFGAVPVTKNSCTIRLSY
                     RLLTEAFRVQTSGPGQSAKERLLLSREEAEGFLADSVVVRVAALAGLPADRDYRITLD
                     ISRIALTGINLAVDDDTGSVSPVKTLFREFLRLTNYGREEWSTTSRSFSLSEVLPRR"
     gene            complement(1815..3578)
                     /locus_tag="PKM94_01145"
     CDS             complement(1815..3578)
                     /locus_tag="PKM94_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD65271.1"
                     /translation="MQKFVLFGTITGAAAACALWAPPATAQPGPEPSYTTYQRATAPE
                     PLFRVAFTLDEISGAAAADSLPAEASVPRAAVRVYPDSVILIDRVAWFDRAGLHLRDR
                     LCPYDAIAETRIIDSPTRVTIDFLTVTGDAERLGRLRRGNLMTFSEPITVGNQQFVRG
                     LVLSITGDIEVLGEVNKDVISLFGDVYVGPGATARGDIAGISGAITVADKGSVYGELY
                     SGGEYFERRRPRFYREENFDFLGAFVYNRVDGATPYLGLKFHDTDSLLPSAWFRAGYA
                     LESERWRTELGIEQPLWRRVPLAAGAAYYRRLASEDDRLLGDEENTAFALLATEDYRD
                     YWEAEGATAWLSVKPAPQWRFETRYRYEETTWRRSYRHLWSLFGGSKLFRENFSQVSE
                     AERLAGAALIDTTALGCLAFVLDWDTHNREEPFAFSGWHATAELEWSDRDFSSDFDYR
                     RYTLTLRRYQKVHSHGMLLLRAMHGNSDGFLPMHKRFYLGGLGTLHGYDFKEYAGTRF
                     WMGNAEYRFEFPRSDIGASVFWDIGQIADGTKLDSAVDIKQSIGAALTVGADFRVSLA
                     RRLDRSFDNDPVVNVRLDFVF"
     gene            complement(3717..5045)
                     /locus_tag="PKM94_01150"
     CDS             complement(3717..5045)
                     /locus_tag="PKM94_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65272.1"
                     /translation="MRERILVVDDEPHITAAFASLLQGEGYRADTASSAEEAQRRVDS
                     ARYDLVLLDLNLPGRSGVEWLADLNRLGRRLPALVISGQSDIATALEAVRLGALDYLE
                     KPVAPEKLLTAVRAALLLSAADRQRALMIDELDRQSPIIGESAAIRKVLRTIDQAAAT
                     DVTVLFTGENGTGKELAATRLYLASPRRDKPFIKVNCPGIPPTLFESELFGHARGAFT
                     GAVRDFPGKFALADGGTIFLDEIGDLPLECQAKLLRVLESGEVERLGAEERRTVDVRV
                     AGATNRDLPALVADGKFRADLYYRVSVFVIPLPPLRERREDIPLLAGEFLRTLDPSGQ
                     TRLSPESAALLATLDYPGNVRQLRNLIERLTIVCAGRRVEPADIAELSPLTGLPGSAS
                     VSLAERVAAYERELIRQALREAGGNIAAAARELKVDRANLSRRIKDLGLK"
     gene            complement(5038..6486)
                     /locus_tag="PKM94_01155"
     CDS             complement(5038..6486)
                     /locus_tag="PKM94_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65273.1"
                     /translation="MHSAFGSRLFRLFLLFALVPAVVLVTVGYYLAVEPGLERGPADD
                     AVVARLHEQYGALLDSTITAILGQPNAREILESQGFFMLFAGGKVQSVWTQREVVHEV
                     AAPVARAVASGRDHGTAPVGERLYRYASIRRGDTLLVGGLAHSPEFAALLESYRGVRA
                     DESAAPGLHTRYAVFLALLFGLVAAATVGLAYVFSSRLARHLARPVAELDRAARQIAA
                     GQFGITVAEQGVGEIRALIGQFNAMSQQLASATARLVQAERVAAWQHVARRFAHELIN
                     PLQPITVSVYQIRKALSGTPSFELVQRPLDAVAEELQHLTVLADRFSRLAKLPAPRLE
                     TVDLRAHLTSVADLYRGKLAGFAFTLELPEEPVAAAIDPTYFREAIHNLLQNAIEATP
                     PGGRISLRLEAGPDGAVIAVEDAGRGMSAEVAREARLPYFTTRPHGTGLGLAIVEKTV
                     SEHGGRLEVRSVENRGTTVTIVLPHAGEPHDA"
     gene            complement(6490..7059)
                     /locus_tag="PKM94_01160"
     CDS             complement(6490..7059)
                     /locus_tag="PKM94_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD65274.1"
                     /translation="MDKKYHDFDDARLRDLKARHCGYRFCPRCARPLAAATIDGVSRL
                     ACPDSACGFVFYQNPIPAAGGIVVEDDRVLLVRRAHPPMVDWWCFPAGFMEWGEHPAQ
                     TAVREVAEETGLIVRLTGFFEVYSGTDDPRVNAVLLLYLAERIGGALRPADDAAEARF
                     FSFDDLPDRIAFESHRQALADYDRRVRRR"
     gene            complement(7040..9328)
                     /locus_tag="PKM94_01165"
     CDS             complement(7040..9328)
                     /locus_tag="PKM94_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD65275.1"
                     /translation="MNFELVQIVLFLLTGGFLVFLAVTIVRDNFANRLNRVTATMLFF
                     AGLGPLFLALGAIVRLSPDTALGFEESTLYHLQHLWEFFFPLLLLFAWIFPEDRLREF
                     KHNRLRYLIFAPPALHLALVLLYNQFTGVLRFLQIEPGDSGFVSIILNPLAKVLSFLL
                     LSINTIRAYDQQIFGALNALYVVVAVYFLESGRRMVTNPRLLTQTRVLFRGMRIGLAP
                     YVIAFAGGLLLPRLFSDDVQAVLVVAGALVGAVFLAYATIRFQFLDVQLRFRQSFVYT
                     ITSGLLVGLYILLVVESRSFFTPLFGAQAQVVSYAFIIVILLLFQPINNAIDNLIRTL
                     FIRTRTDHRNIIERFSRQVISLFDPVKLRGIIEDTLKTALLVERVYFVLFDDEVGEYA
                     LTRSQDQPRRIVLSRDDLLLRGINLLDSPAFLQTLGDYLVNSPLAELLTERQVKLVLP
                     MKDAKHMLGFLALTDKVAGYRYSSEDLNLLGVLSNQMVTALTNARLYVDSLERLRLEE
                     EVSLARQIQLDLLPAAPPKLELCEIAVHSTPSRTVGGDFYDFIPIDDARIGMVIADAS
                     GKGMPAALMIAQIQAIIRSEVNNGNPIGAIMTHLNDQVVAATSSEKYVTLFYGELNRR
                     TGRLEYCNAGHNYPVLVRSDGAVELLVQGGPIIGAFPEMRYESADVQFRPGDVLFLFT
                     DGLSEAMDGDGREYGEERVRRFVREHRHREAGEILEAILEDVRAFDATFPPRDDTTII
                     TMKSNHLRAAEASRESHGQEVS"
     gene            complement(9358..9780)
                     /locus_tag="PKM94_01170"
     CDS             complement(9358..9780)
                     /locus_tag="PKM94_01170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65276.1"
                     /translation="MKRPVIKGNTIVVPSSHDYLPTVDEFVEGILRGWGTDESVIADI
                     AISVSELVNNAIVHGGRVNPEQQISIEVTRSDSAAQIRVSDQGQGFDPDQVSDPLAEE
                     NLLKEVGRGIFIVRSLMDSVDIDVSAGGTSVTITKTIR"
     gene            complement(9871..10209)
                     /locus_tag="PKM94_01175"
     CDS             complement(9871..10209)
                     /locus_tag="PKM94_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD65277.1"
                     /translation="MKMTDREQDGVVVLEPKGKIMGGPDASLLHDKLHDIIERGRRQV
                     VIDLGKVDWMNSTGLGILISSYTTLRNHQGELKLANVTDKIQSLLTITKLVTVFDAYD
                     SVDDAVRSFR"
     gene            10636..12486
                     /locus_tag="PKM94_01180"
     CDS             10636..12486
                     /locus_tag="PKM94_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65278.1"
                     /translation="MRPLTALFLPTVLLLASAVAAAVPSTMTFQGRLTDDLGQPLDTT
                     VALTFRLYVASSGGSPVWVETHDDVVISYGVFEVQLGETSPLTRSHFDGSPRWLSIAV
                     DGGAESAVRLPVNSSAYTIRANYADTSGRAKYADTAAFAVGVGNSGWADDGANVRLVT
                     ASDWLGIGTASPEDKVHIESSLSGGTAFLKLETSHASNWGETGLRIETPQNRWHLRMD
                     DATNNNIPDGALGLRSQDVGTEIMTWTSDGKVGILNTLPEYPLDVRSTAVAIYGRTTG
                     GLGTAGVYGRAENDGFGVMGTSTSGRGVYGASASGFGGYFLGPTTYVSGNLGVGTETP
                     THKLTLNGTLAYQSAGTTKYHVAYYGGGLDFCETGVGDYRLMIEDGGEVGIGTANPTA
                     KLHVMGNVKVRDTLTAGAIFSSSIVDEFGLASAKNALYESTACTGSWKSCLSRTIRVP
                     APGWVLAVATARVKLDHPGSGSTDVSVALSDDSTTADDWWYANWALPGAAVAGNYYST
                     LNPMRLFRTDTAGVYTFYVNARRLDGEASVLGAQMHCFFIATSYSFDEAVAGGEGSGA
                     QPAAPVLTELGDVPAPAENPGAADVASILAELSALRQEVESLRNKVEANR"
     gene            complement(12571..13005)
                     /locus_tag="PKM94_01185"
     CDS             complement(12571..13005)
                     /locus_tag="PKM94_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD65279.1"
                     /translation="MNSRLVVRPNLCIGCRTCELACSFTHSADGKPGRSRIFMLDGGH
                     EKLWVTVTCLQCDDPACVKSCLVGALRRNEQTGAIELNQETCVKCMACVAACPFGCAI
                     LDHTHDRVVKCDLCGGDPACARFCPTKALDYRPIAQAAKKAG"
     gene            complement(13002..13787)
                     /locus_tag="PKM94_01190"
     CDS             complement(13002..13787)
                     /locus_tag="PKM94_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD65280.1"
                     /translation="MATVTIQVNGRAVRAEKGEMLLAVLRREKIDIPATCNHEAVEAF
                     GACRLCTVEITRDEWKGWKNYVTSCLYPVEAGLIVRTHSDTVIELRRTLLDLQLARHP
                     RTPLIQQMAAEYGVLKTSYEEAPDGDDCILCALCTRVCDAMGFRAISTVGRGHGKRVA
                     PPLDQPPPDCVGCLACAQICPTGFITYKEFGAGREIWGKQFQMVTCTRCGAPTITREF
                     ADNLAARREIPREYFDVCDACHRAELAGTMGRIAQWNREEAAR"
     gene            complement(13791..15647)
                     /locus_tag="PKM94_01195"
     CDS             complement(13791..15647)
                     /locus_tag="PKM94_01195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOD65281.1"
                     /translation="MKITSPAQLSRLQTDLAAQDKKLGKKVMICCGPGCLANGATKLV
                     QAFRSELEKKRIRDFRVEALKETGCHGFCEKGPLVVIEPQGTFYTRVTPADVPEIIEQ
                     SIRGNQTVERLLYRDQATGEPKTTYHEIDFYRKQTRIALRNLGKIDQYSIDEYIRVGG
                     YQALAKVLTTMTPDQVITEVKASGLRGRGGAGFHTGRKWESCATADDWPHYVVCNGDE
                     GDPGAFMDRSIMEGDPHSVLEGMIICAFAVKSKDGYIYVREEYPLAVQNLQRAITQAR
                     AYGLLGEGILGTEFSFDIKISRGAGAFVCGESSALMKSVAGEVGEPRAKYVRSVLKGL
                     YDKPTVLNNVESYANIPVIIAKGGAWLAAIGTPKSTGTKAFSLVGKVRNTGLIEVPMG
                     TTLREIIYGIGGGILHDRPFKAVQTGGPSGGCLPADKLDLPVDFDTLTDAYSMMGSGG
                     MIVMDERTCVVDVAKYFLHFLVEESCGKCTPCREGLFQLHELANRISDGRGTEADLAL
                     MEKLSENIIIGSLCGLGKSGPNPFVSTIRYFREEYLAHIRDHKCPAGVCTALIRYDIV
                     AEKCDGCMACIHACAVNAITGRKGKVHVINQDICTKCGACMAVCRRDAVEVA"
     gene            complement(15644..16126)
                     /locus_tag="PKM94_01200"
     CDS             complement(15644..16126)
                     /locus_tag="PKM94_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD65282.1"
                     /translation="MKHDFSRLPDIIGRYPAEPGSLIAVLQDIQKEYNFLPCEALTAA
                     AQALHVPLSKVFSVSTFYNAFCLTPRGDKIIRVCMGTTCHIRGAKQLLEQLETRLGIK
                     AGQTTRDMKFTIEVVNCVGACAMAPVVIINDEYHGDCKVSDARRFTIGAKKDRKEAAE
                     "
     gene            complement(16123..16686)
                     /locus_tag="PKM94_01205"
     CDS             complement(16123..16686)
                     /locus_tag="PKM94_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD65283.1"
                     /translation="MAQYEVMFAGFGGQGIMTAGQLLAYAGIKEGKHVAWIPSYGPEM
                     RGGTAYCTVVVSDARIGSPIVTTPWSVCAFNRPSFDKFEPRVRPGGLVLVNSSLIDVT
                     SSRTDIRQLLVPANEMAIAAGSPKSANIAVLGALIGATGIVSFDTVRATVREKMAFKA
                     SVLEANLRLLEEGRRLAAARVGQEDTV"
     gene            complement(16686..17453)
                     /locus_tag="PKM94_01210"
     CDS             complement(16686..17453)
                     /locus_tag="PKM94_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD65284.1"
                     /translation="MGHATTTIFRRPTALQETVTHYCPGCTHGIIHRLVAESIDELGV
                     RERTVGVAPVGCSVLAYNYFNCDFLEAAHGRAPALATGFKRLRPDLIVFTYQGDGDLA
                     SIGMAEIVHAANRGEKITTIFVNNAIYGMTGGQMAPTTLPGQKSTTSPNGRDVALTGW
                     PIRVSELLATLATPLYLERVAVHTPRHILTAKKAIRRAFEYQVKGECFSLVEVLSTCP
                     TNWGKTPEEAERWLQDNMVPYYPLRRFRTPETTAEVR"
     gene            complement(17461..18624)
                     /gene="vorB"
                     /locus_tag="PKM94_01215"
     CDS             complement(17461..18624)
                     /gene="vorB"
                     /locus_tag="PKM94_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOD65285.1"
                     /translation="MAKILMKGNEALAEAAIRAGAEGYFCYPITPQSEVAEYMAKRMP
                     EVGRVFLQGESEVAVGNMLFGAASTGKRVFTTSSSPGISLMQEAISYMAGAHLPAVIV
                     NIMRGGPGLGGILPAQSDYFQAVKGGGHGDYRVLVLAPSSVQEAVDLMMLAFHLADKY
                     RNPVMLIGDGMIGQMMEPVEFPEEYREDPLPPNDEWAATGARGRRPRIIKSLFLDPEK
                     LEKNSWDLAEKYARMRREEVRCECYKVTGKNRILIVSYGTMARICQTVIDELEAEGVS
                     VGLLRPITLFPFPADQIRAEIEKKNIEAVLTVEMSTAQMLEDVELAMQGKKPVAFYGR
                     TGGIVPTPEEVRDKILAMLGRTGAPAAKAAGRAARKTAARKTDAQKKAAGKKR"
     gene            complement(18645..18878)
                     /locus_tag="PKM94_01220"
     CDS             complement(18645..18878)
                     /locus_tag="PKM94_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD65286.1"
                     /translation="MPGVRIDKNHCKGCELCTKACPQKILSMSDDITVRGYFYARLHD
                     PSRCIGCRLCALTCPDAAIEVHAHGTTFVLVDY"
     gene            complement(18900..19631)
                     /locus_tag="PKM94_01225"
     CDS             complement(18900..19631)
                     /locus_tag="PKM94_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiA"
                     /protein_id="HOD65287.1"
                     /translation="MARPTPTYPSPSFAKRVLTIVGGYGSGKSEVAVNLARQLALTGG
                     KPVAIADLDIVNPYFRSREATRQLSELGIQSIHPTGGLAHADLPIILPEVRGAMEQFG
                     GTLILDVGGDDVGARVLGSLADAVPADDYELLLVLNAHRPFTATVAGCRALMAKIEAT
                     SRLAFTGIISNTHMLEHTTVETIREGLRLARALAADTSLPIAFVAALEDQVRQINPEE
                     IDVPVLMLDRLLLKPWERPSRRPGG"
     gene            19902..20723
                     /locus_tag="PKM94_01230"
     CDS             19902..20723
                     /locus_tag="PKM94_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65288.1"
                     /translation="MLRAPFPCIFLAVALGLVLAACQSDKKETSPSAADDPYAGWKTY
                     TNGTLRVHYPEGHPMAAHVEEMAHVFPALMRRDAQFFQVPVPTDTIEVYFYTGVGHGH
                     KITGTYYPYADTSVIHFWLPSNYGPIMAQLMLKRWYPGEPKFRFLKHGVRALLEFAGV
                     NYHQRTQELIDSGKFVSLAELAADTASDDYPDTESEQIRRALGASFVDFLVVHEGIRA
                     FGMMYSSPLPFDSTVQRMFSVSADSLQQLWLNFVPLGAHPEELGRLPREAPPAVP"
     gene            20786..21553
                     /locus_tag="PKM94_01235"
     CDS             20786..21553
                     /locus_tag="PKM94_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HOD65289.1"
                     /translation="MPEETWSTAITDIAPGRIRVRGYAITDIMEKLSYAEAVFLILKG
                     QLPSPAEAELMNAVLVSSIDHGASPPSVLGARTVLSGGNSLNAAVAGGVLVIGDTHGG
                     AIEQSAKLLQEWAAKSGDVETLAGELANWLKVNKMRMPGFGHRLHNIDPRTGKLFALA
                     DRHGYRGRHIDLCRAIEKALAEKLGKELPINVDGAIAAVISDMGFDWRLGKGFFIISR
                     VPGLVAHVYEEMTREKPMRRLGPPPFKYDGPPDRDIP"
     gene            21698..22357
                     /locus_tag="PKM94_01240"
     CDS             21698..22357
                     /locus_tag="PKM94_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOD65290.1"
                     /translation="MTADTDEFRRRLACFVPDGHTRERLLALWAAAIALADEPAMSSA
                     FHLAAGRGLDRASLYEIVLQSYLFLGFPRMLIAAEHLHRQYKEERPPASVEADFAAEA
                     WFDRGTALCRLVYAENYNRLRERVEAMAPEIFRWMVLEGYGKVLSRPGLTIIERELAI
                     IACLAIENRPAQLHSHVRGALNVGTAPGLVRAVVEDIGPAAGEGYQTARSICRRLRIE
                     L"
     gene            22357..25911
                     /locus_tag="PKM94_01245"
     CDS             22357..25911
                     /locus_tag="PKM94_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOD65291.1"
                     /translation="MRRLRKIILVLLAVAMAVLVGGAVYLVYLGGIERLANDRLRRMF
                     AQQNNLDIRIGKIGGDMFSRLSLHDVEIYYVDSTHRYRLARFPELRTAYSFRNLWNRR
                     YILNFLYIDSLDVTLVQDSVAGWLLPPLTRPAGAAKGPSDLPSFLVSQVTVDHGRLTV
                     VRGGDTTTVRDVAFAAAVEGDQGLYTADLARLAFSSDLPGVKLDAGQGKVTYADRRLL
                     FTGVSVLSGSTRLSLEGNVRFEDEPAGEVRLSGDDVDVADLARYLNVSLPGTVDLAGE
                     VSFVGRAAEGSLNLAGSLSIFDMQNLVIDFHYRDNLLTLDSLYGSLLGGCSLDGSGDI
                     DFAARPNTYRLEADIRNFNLDRLVPGTFESDLTGRIALEGESFSSRSLRLTTVVGLYE
                     SRFGDFPIQQAYGTMVITADSLVFVDPFRVDYFENVIWAGGVIDYDDDINLSVQIDLN
                     NLDRYRNTRLFIEEPGGRGYAEATLTGRTVDPDLRGWFASDSCWIYGLFADTLYAEVD
                     LDRFLAGRRGTVEVWFDRGSTWGLPYDTGYAWLRIDSNLVYIDTALVRNPYVTVAGAA
                     ALDYLAQPQQLRIGALHVNLLDRSFSNPEEMLIEVDTAGFMFRRAEIGNADARLSLAG
                     RINYDESMDLRLTLERLPIAPWLSLADSTIPMDGYLSTTAAVAGDFRSPVIRLEGAVD
                     SLTYAGLELGDAVAALSYADARLDVDSACIYSDSGAYRARGVLYADLAFTADSLERFP
                     RRPMAIRIDARDRDFDLVSLLLPSVEDLQGDFFADFELSGHPRDPHVQGEAYIKNARL
                     KYFDLENWIFADSAGVSMHDDSILIHRIEAYATDRNDPSGVGRRHYAYIDGSLQLLAL
                     DSLAYDVEVRLDNEFPFTYELEDIRGKVEGRLFVEGPTPPTVSGDIQLISAQYRVPFA
                     EEGEGSPILRALTSQNTWDLNVNVEILSNFWIKNEDIDAEFAGEINLVRERGVYRFLG
                     EMEVLRGRGFLFDKTFRLDPGSKVTFQGSDTLKPQLDITGSTRMTAVRATTGDEGPVT
                     EAIDLCVHVGGTLETPEINPCGGEDIAQSDLLPLIVTNYYGGDGLAVGGRLEERVLGL
                     GYAQFSQIGGRQLNRIGVETFEIDPVYSDQLGPWNARVTLGKYVGEGLYVYGRSTISG
                     QTRQEYGFEYRWFKGVLLEGRRDEDELYHLNLRLHWEW"
     gene            25905..27839
                     /locus_tag="PKM94_01250"
     CDS             25905..27839
                     /locus_tag="PKM94_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD65292.1"
                     /translation="MVRIARRVLPVVLALAAVGAFGPAAAVDRDMLRLIKDNPPIRDI
                     VIDGNAHYSDGRIREQMYARPRTFWAALKGDRRSRLQRETLDRDTLEIKYLYLRSGFL
                     GVRVSESFEPMETGAGVRVRTVIDEGRRSVYGDVRIQGEYPPRLESRFQRAVRGLKQG
                     APVNYFDLVETGFEMKAVLANNGYPYAAVTPEVDTGGPDGATAITYAITADSLVRFGG
                     VSVRGVKRYPEYTAVRELKFKPGDVYRRQDLIDSERRLFESGYFSTLQLQQEAQQSDR
                     YRPDFVLQVRERKPYYLSFTTGAGQSEAADLTWSVTSGAGKRNLFGSRRLEFYNRVEF
                     SLGADARLTDHLYRLRYTEPWLLGFRMPLAVTLEYQPRIRHEAQDFDVEEWNASLSTT
                     RRVGRLTDVTAGIEYQSVDISGVPAELEEAVRAEVEGLSIRRRLYLNLRRDSRLDVFV
                     PRQGSVRDLRTEYYGGFLGGDEHFYRLEGSWSFYRPVWPGWISATRFRSGYAHAFGDS
                     ELVPSKDRFFLGGANTIRGFAENTLAPTLADSLPGANFTVVCNQEFRWKTLQVFAVVP
                     LLNKLLENMPLWQTVFIDIGNGFTRVSEFSFSRLAYSYGTGVQIISPAGPIRLDYARR
                     IETDDIPFDDRWHFTILFAF"
     gene            27960..29135
                     /locus_tag="PKM94_01255"
     CDS             27960..29135
                     /locus_tag="PKM94_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOD65293.1"
                     /translation="MDQIYLDHNATTPVDPEVVDAMLPYLSHTFGNASSLHYFGREAK
                     AALERAREEVAAFISCDPGELYFTSGGTESDNMAVLGAAFQRRDRRRHAVYGATEHHA
                     VMEACEYLVHRHGFTADAAPVDSEGFVSPRRLARLVTDQTAVVSIMHANNETGTIQDL
                     KPLVQAAHEKGAWFHTDAVQSCGKIEINVRDLGLELLSLTGHKIYGPKGTGALFIRDG
                     IKLTPLMYGGSHEKKRRPGTENVAGAVGLAKALEIARRRMEEDWIRLSDLADFFIDRV
                     LTTIPHTYLNGTRMGRIPQVCNISFTGVEGESILLALDLEGVACSSGSACTSGATEPS
                     HVLVAMGKDRVVAQGAIRFSLGRSTNREQLDYVLAKLPPIIERLRSLSPLYQERMKS"
     gene            29178..30374
                     /gene="mnmA"
                     /locus_tag="PKM94_01260"
     CDS             29178..30374
                     /gene="mnmA"
                     /locus_tag="PKM94_01260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOD65294.1"
                     /translation="MPPRVLVAMSGGVDSSVAALLLKEEGYEVVGAHMKLWDYVDVGG
                     DRHRDGRCCSLDAVTDCRLVCDRIGAPFYVLNMSAPFRAAVIENFVSEYRAGRTPNPC
                     VLCNTDIKWSGFLHKAREIGCDYIATGHYAVIEQGENGRWRIRKGVDATRDQSYVLWG
                     VSQDALAMTLMPLGRRYKKDVRAFAARVGLRTAAKPESREICFVADNDYRRFLREWEQ
                     KHGLKRRPGEIVHEDGRVLGRHAGTVDFTVGQRRGIGLEHPLPEPVYVQRIDAISGRV
                     TVGSNSSLFRDELVAERVNWVAHPPPARDQTSTYAAGENPGGPAPAAAESPAAGAATA
                     TGVSFDALVKIRYLHTPGHARITCLADGRVHVKFTDRQRAITPGQSVVLYDGDIVLGG
                     GIIAGP"
     gene            30472..31149
                     /locus_tag="PKM94_01265"
     CDS             30472..31149
                     /locus_tag="PKM94_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOD65295.1"
                     /translation="MPHIQWLGHSCVTITEGPHKLIIDPFLKDNPKGAAKPETIDIGW
                     ILLTHGHGDHVGDAVAIGKRTGATVIASFELANLCAKEGLKVHPMHIGGSHEFPFGRV
                     KLTIAHHGGGYGPDASRYTGPPVGFLVTIGGKTIYHPGDTGLFLDMRLIGEMNRIDLA
                     FLPIGDNFTMGIADAVKAVEFLQPKVVIPIHYGTWDLIAADPAEFAGKVRGARVELLS
                     PGGRYTL"
     gene            31339..34170
                     /locus_tag="PKM94_01270"
     CDS             31339..34170
                     /locus_tag="PKM94_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOD65296.1"
                     /translation="MKKLLKIVAWIVGAIAALLIVAIVGVMVFFPKEKAKALIVERGS
                     QALGRPISVAAVDVSFWGGLGVVLRGVAVANPPDWPTADTMLTAEQIDVKLKLLPLLS
                     GEYRLARLVIDRPVIVLHKTGSGAANYAFPVTDTARAVQALLEETRAAAVAVSFDRLA
                     VNRGRLTYIDDSTGVSVALNDFTLSTALANPRANVFRSEGNLEIPQLVAVLEGTVPPF
                     ALGLRYRAEYDLGAKKLLLDDSRVRINQLNFALSGELSHGGAAPVARGHIASDQIAVR
                     DLFSLLPAPQLAMLQDYTIEGDFALEADVRYDVGAPDTLDYSGSARISDMLLEYRQVP
                     GQLRVPSATVDFKRDNARLNISDASFAGKPLKGYVYVENFAAPVVNAELTGEINLGLL
                     RPFLPAANRPELVGDASFSVKASGPVRQVEELEVSGNVRVANGRYAAAMLPEPVESFS
                     LDVYFDKSVVRVNALDARMPSGNLSFKGRVNDLLPYLMADSLHPVKTGPAVDGTLEAQ
                     VNLAMLNPFLPKQGKPEIAGKLQTSLQLAGDILEPSGLRVRGTLAVGEGAYRDTALAE
                     PIEHFDTRMTIIPDTIRIDELNIRFTTSDLSLTGSLIDPFPYLLPLKSVDRTRAAKPF
                     LQFAMTSHRFNVDSLFPDTLPSGDAADGGPAAGDTLVPFILPDIDGRGVARFDTVIYW
                     NVPFSDLTGDVTIKDRVVRVTNVVGRVYTGTVTGETSIDLNNWLAPAYDGRFNATDIE
                     ANDFARRFTRFGDFVYGKFNLDGSFQASGWDADAIMYSLNMNSAARMREGRLETSGAV
                     LTALQTVGSLVGEQLGRTFNLRNARTDIAVDSGRVRFDKLSAALGDMGDLELNGFYSF
                     AGEVNYTGTLLLSPELTQKFLSQGVLGEVSSFLGGSSAVKQVKLPVTIGGSLDDPKVD
                     INLKSITEQAGKSAVEGAADKLKGLIKKP"
     gene            34418..36280
                     /locus_tag="PKM94_01275"
     CDS             34418..36280
                     /locus_tag="PKM94_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD65297.1"
                     /translation="MPTVRQQKPGAAVEMNARAIARCGVCKVDLKGRFVFVDEQAQQL
                     FGCPGEELFGKALIDFVDEASRAVVNEILSRRNHYEITFDHIGLTLVVPGAAAIRAGA
                     IVTLNFNAGNPVNFQFIFLADNAVAVDLVRDPHAAAYRRLVAYLDGRDLTADWRGYLG
                     ELQSFSGAVQVCAYLVSPDRLEPRSSALDAGDGVTSLPSIDPTGPLHRQVADTGETYD
                     FTDQRSIQRAVELGAPAPNEVIARLALDEGQAALIRVIFPSDIADNDARVQADRTRFA
                     LELTQRLLAPPPPSADDRTADNIQFAIGLLDGLGIGAMMADSRGKTAVYNPVLARLFQ
                     VEDVSVDLVELAEILSGGAAEERQRLDDFLRGAARPSTGALELARVELPAGRPGLLAV
                     LPLAENPNDRTAFVAIVPGALAADAADVLAFGRTLLSRLGADTAEAVALSARLGQRSA
                     ALDAEGKDQLHRLTESLQDLGRLTQDLRSALDISSRVQAPELTDLNIVLHRVVNHVNR
                     GRGGQPLRVHTDLMPKVSTSRRAVTLALENLLQAVATGADRATATVRIAAELSGPSAC
                     RLTITVDSPLDRSHNDFAFGFSAARLLIRSIGGTLREEPRTAGVGFVIAFPVQP"
     gene            36452..37327
                     /locus_tag="PKM94_01280"
     CDS             36452..37327
                     /locus_tag="PKM94_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOD65298.1"
                     /translation="MDSMTGFGKAEFKSALGTFTVEVSSVNNRFLEVSARLPRQFSAM
                     EPKVRELVGRHVNRGKVYIYVGFSEGEENTDRFSINVKAAAAYARQLAALRDQLHIGG
                     EVTIGDLLVFPEVVKSDETAFEDEEIWEQLEAPLAEAMTALVRMRRREGEAMARDMKQ
                     RLDQIKEITRAVEKEAAQSVDRYRDRLLVRISELAETIQLDPNRLEQEVALQAERSDI
                     TEECTRMFSHIDQYDADLRRDDPVGKRLNFILQEMNREANTIASKCTEASITRLVISM
                     KEEIEKLRELVQNVE"
     gene            37347..37961
                     /gene="gmk"
                     /locus_tag="PKM94_01285"
     CDS             37347..37961
                     /gene="gmk"
                     /locus_tag="PKM94_01285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOD65299.1"
                     /translation="MTPTGKGKVVIISSPSGGGKSSICRQLLERNPAWRFSVSYTTRS
                     RRPDEVHGREYFFVSADEFRRLAKQDFFAEHCRVHLYEYGTPKKPLEDVRAEGGVVLL
                     DIDVQGAEKIKAVFADAITIFILPPSIAALRSRLSTRGTETQEQLQVRFENATREMRL
                     WRRFEYAVINDTLATAVREVESAIIAHHCRTDRLAPEQIENIIG"
     gene            37996..38217
                     /gene="rpoZ"
                     /locus_tag="PKM94_01290"
     CDS             37996..38217
                     /gene="rpoZ"
                     /locus_tag="PKM94_01290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOD65300.1"
                     /translation="MKKVPIEQIEKTGLNRYEAVIVASKYARKLNTKRLTLLEQMEDN
                     PEIDIDSRKITMVALNDLIEGKVKFERPR"
     gene            38264..40561
                     /gene="topA"
                     /locus_tag="PKM94_01295"
     CDS             38264..40561
                     /gene="topA"
                     /locus_tag="PKM94_01295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOD65301.1"
                     /translation="MAKNLVIVESPAKSKTLARFLGKDFDIMATIGHVMDLPKSKLGV
                     DVKNNFQPDYTVIEGKQKVITELKKAAKKAETVYLAPDPDREGEAIAWHVANSIGNSH
                     KAKFRRVTFNEITKGAVLEAIANPREIDMNLVNAQQARRVLDRIVGYTVSPFLWKTVA
                     RNLSAGRVQSVALRLVCEREAEINAFVSQEYWQLTAELETAGKERLEARLYKIGERSV
                     VKPGDSNDNGNKMSIGSENEVRGYMAELEKESWVVAEIKNTERIRRPSAPFITSTLQQ
                     EAAKAFGFSPKVTMRLAQELYEGVDIGQEGPTGLITYMRTDSTRIANEALKAARELIA
                     RDYGKAYLPPKPQVYSKKQNAQDAHEAIRPTYLALPPDKVKRFLNARQFKLYSLIWNR
                     FVASQMNPARYDVETIDIAAGRFLFRAAAQRLTFDGYLKIYHEEVEADDNGNGNGAAA
                     ALPRLAEKDPLRLLELRPNQSFTKPPARYSEAALVKRLEADGIGRPSTYASIISTLRD
                     RKYVKLEQRKLAPTELGLTVNKILVENLPKLFNVKFTAEMEKELDLIEDGTDDWVKVL
                     NEFYEPFKKSIEVLKGRQKEIKEAMTEETDIKCEKCGSPMVIKWGRNGRFLACSAYPE
                     CKSTRPLPEEEAARKTDEVCEKCGAPMVIKTGRFGRFLACSAYPNCKNTKAITLGIAC
                     PQDGCKGQLVEKRTRGGKIFYGCSAYPSCKFATWDKPVPHPCPHCAYPFLVAKESKAK
                     GEYLQCPRCKAVIAPDSVEAHTPAH"
     gene            40681..41595
                     /locus_tag="PKM94_01300"
     CDS             40681..41595
                     /locus_tag="PKM94_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD65302.1"
                     /translation="MLQQDLTAYLGALAEERRCSPRTVEAYRRDLEPWLAFLAEQHAA
                     LPGGRPNDPLLLRVYLRRRAEAGVSNRSLARFLSALSGFQKFLAARGRAEVWLFKLPR
                     MKYPARMPRFLPQKEAGRLFEEPPAQQPERTYFYRRDFLMIALLYATGIRREELAGIR
                     PGDLDLRQGLITVTGKGNKVRVVPIGDATLKDVRAYLDDRARFLADRGTACDALLLNR
                     YGGPLSVRSIDRLVKRFGRRCGLDFTPHALRHSFATHLLENGADLMVIKEILGHASLS
                     TTQKYTHVTAEAMKKVYRKAHPRSGSKE"
     gene            41606..42142
                     /gene="hslV"
                     /locus_tag="PKM94_01305"
     CDS             41606..42142
                     /gene="hslV"
                     /locus_tag="PKM94_01305"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOD65303.1"
                     /translation="MRTRSTTIIGIIHNGRAAMAGDGQITFEDTIVKARSSKIRTMRE
                     GTILCGFAGTAADALALYELLEKKTDEYAGNLARAAVELAKEWRTDKALQRLEAVIAV
                     TDGRHIFLVSGNGDVIEPDDQIVAIGSGGSFALAAARALVWANPKLSADKIAVKALEI
                     AAGICIFTNAHITVETLG"
     gene            42139..43491
                     /gene="hslU"
                     /locus_tag="PKM94_01310"
     CDS             42139..43491
                     /gene="hslU"
                     /locus_tag="PKM94_01310"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOD65304.1"
                     /translation="MNQSYPTPREIVDHLDKYIIGQTQAKKSVAIALRNRWRRQQVPA
                     PLRAEILPNNIIMIGPTGVGKTEIARRLADLAGAPFLKVEASKYTEVGYVGRDVESMI
                     RDLVDIGVNMVKSEKMEELRARAARGASERLLDLLLAPAEPEAPAGDGGERPASDSVR
                     TTRAKLREKLIGGELDEREVEVEAPQSQFPTIEIFSPMGMEELGVNFQELFSGLMPKK
                     TRRRKMSVREAHRFLTAQELDRLVDMDEVIAEALGRVQEAGIIFIDEIDKIAGGGDRT
                     AGPDVSREGVQRDILPIVEGSAVMTKYGMVRTDHILFIAAGAFHSARPSDLIPELQGR
                     FPIRVELDSLTADDFERILTEPKSALVLQYQALLKTEGIRLEFQKEAVRRIAEFAERV
                     NSEAENIGARRLQTVMTTLLEEILFEPPEKKRVVITARMVEKTLSAIIENEDLSRYIL
                     "
     gene            complement(43516..44271)
                     /gene="liaF"
                     /locus_tag="PKM94_01315"
     CDS             complement(43516..44271)
                     /gene="liaF"
                     /locus_tag="PKM94_01315"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HOD65305.1"
                     /translation="MTGRRATFGIILILLGFLFLLNSLDVMSVGEVLRYLVPLGFIAL
                     GIWLIIRRKQQDAALSFEAASAPPPPPPPSPSPAGPAPQTFWAHEGAQSTAAPAGDAP
                     AGGSADPAAPAGGPQFQSPEYGVMRDGKLRYSKLIGDLTVDLRDRAVQHVEVSMGIGD
                     LEIRVHGARLEGGLCRMIVSGFVGDIRILLPPDMAVLAHCSNFIGDIDLLGRRVSGFS
                     SNLDAQTANYAAAERRLYIAANSFLGDIKVYQV"
     gene            44484..45386
                     /gene="argF"
                     /locus_tag="PKM94_01320"
     CDS             44484..45386
                     /gene="argF"
                     /locus_tag="PKM94_01320"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOD65306.1"
                     /translation="MARSLTQITDLTADEVRRIFDLCARMKSGQIAPKPLAGKAVACI
                     FTKPSLRTRISFEVGINQLGGHALYITDNEIKLGERETIQDAARVLSRYIGMIMIRTF
                     KQSDVEGLAKYASVPVVNGLTDLVHPCQILGDYFTVMESFPGRERFKIAYVGDGNNIV
                     NSWLNLASLIPMDLRIGTAADTQPDGAILRRAQANPASRVLLTEDPKEAVAGADVVYT
                     DVWASMGQKHLAEQKAEKLKKYQLNSSLLALADPKAIVLHCLPAERGREITDEVIDGP
                     HSRVFDEAENRLHIQKAIMTFLLQ"
     gene            45439..46287
                     /locus_tag="PKM94_01325"
     CDS             45439..46287
                     /locus_tag="PKM94_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65307.1"
                     /translation="MRSHRSLFLSGAALALAALAFIWGCSDDDDNPTKWGSLEDPQFQ
                     AANTQFEALLDTTITQFTEALGAIQTLPANDDDPIHHGPGDPNGITDSLIVSYSSGWH
                     TIHLEWHSQSGYIAIVNDSIQFLRNGQPTQEGSGLEQLLYRHRWSWTAPDTTVTYING
                     AGYSLFDFDGLHTQEATIDGANGCSVETKFVFADSVVHYDFAAEAALDDVVVTKTGPT
                     WDRGCPTSGRFTATIMMTYQRNDEAPVPTQWNFSATFHNGAVHVVATANGYTWTYDGV
                     ACSQEG"
     gene            46431..46862
                     /locus_tag="PKM94_01330"
     CDS             46431..46862
                     /locus_tag="PKM94_01330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOD65308.1"
                     /translation="MREVPKYHGCFVCGDKNACGLQARFFFDGEKVLTEAVADERFEG
                     YCGIGHGGITAALLDEVMVKAVLAADVCAVTAEMTVRYHRPVYTGDKLFCTGRVVKQR
                     GRAWFAEGEIVDADGRPFASATGKYIEAEEDFRRLLLRSLE"
     gene            46771..47526
                     /locus_tag="PKM94_01335"
     CDS             46771..47526
                     /locus_tag="PKM94_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65309.1"
                     /translation="MPTAAPSPPPPANTSRPKKISVACSCARSSDGAFLGSELHSARQ
                     AAAAATRQVILSSAAAATYDPAGAPVASGTGIAQRSCMEAPVCPLTCYLALRRCPLAI
                     PDPAADDRMVSAIVSFAIPDYGILYRTRMTGDILDLEFRALFAVLGFIQNRLAGEKIE
                     AVRILSSTPEFIFALTPESPHLLSNRKRRNLLRKFCGAIKVTAAYVRQADNRALLSPA
                     DLPAMPRGAEIAFSEEPERRSESGSLPPFGVDI"
     gene            47692..50424
                     /locus_tag="PKM94_01340"
     CDS             47692..50424
                     /locus_tag="PKM94_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOD65310.1"
                     /translation="MKKLFGSLALLLIAATGSATAEDIGWITVRNQFQADLAAQVVGP
                     AYTRDGNRFLAGLSATKEQLLRQSGLAVEIVYTDVDPAGVYRVMPNGPAPRDRMDMQA
                     LGQAVPINETMSLLQTDPAVARELSERRGCHAQALTTLSVRWLYLPTTVATGLLDIDD
                     FPTDTLINLVSQDSIFAFDERLMNFRTRYIFADSIIAALNWIVQKFQSWGYTVTLQPF
                     SYYGTHYNIVATKPGYAEPDKTIVVGGHYDSIVYGDGTDPYVYAPGADDNASGTVAVM
                     EMARVLKDVPLRKTVAFIPFSAEEVGLVGSAYSAAQFAAQGTDVEVMFNYDMIGYDPS
                     NERLIALSAGPANGHYRDLHIATIERLTDLVTMPENSPGGSDHQSFIDQGFPVSNSIE
                     GTFNYPGWHTNLDLTSEMNFPFFTKVVKAGLAALAIVANSARPTEIERLVDMGDGQSI
                     EVIWKSCDPSYRYVVRWGTASGVYTDSAVVPPGGCSYVVTGLTEGQPYFFTAEGSVED
                     SYPSIYSTEDSLTPLVVPRRPSALVADPALQAIALSWRPNTEGDLTGYKIYRKIEDYD
                     YQLLAGGVTGQSYTDSAIAPHSLFTYRISAVDGDAHESELSPESGNYAATFDGGILIV
                     DETSQHPSVPTQAGQVAFWDTILGPTPYSLEVTDFAGAALRRGIAGQYSSIFWFDDDF
                     TNHFLKTSQDSLTWYAGYTGNILVAGLRTVQYWTSSVIYPDDFVYTDFGLASWTENSA
                     FDFAGAHGQNGWPSLTVRPNVFDNLPYVPSLQLAPGATLLYTYNSATDNPAREGQPVG
                     ILKTTPHGARIVLAFPVMFLTDSSATQLMNFVKAQFGESGSVEEYGDVDGSGSVNVAD
                     LTYFVAYMFRGGPPPPVLNNADVNGDCELTVADLTALIGYLFRGGPAPVAGCVE"
     gene            50674..51489
                     /locus_tag="PKM94_01345"
     CDS             50674..51489
                     /locus_tag="PKM94_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65311.1"
                     /translation="MEFHALQHLRIIDQDRIAPFEWDDGGRIDLSPEGPGIFRDPLLV
                     TPLEGDDFLLLDDSRTYYSLVDADFEHLPVQICTPVELMVDSDPLSLTRFTHDDLTRV
                     LARFPDRIEILPAEKTVPDSPDHLDLIFGFVGAAPIRVRIRHSSLTGCPVPFDAVFRA
                     ILESGAFYPVVRDGGEGEWGDHSGPHSGTVSLPTFTLEDLRRAVSSGRLFPPGLIRVQ
                     AVCRVLNIDFPVPVLADRSTLEQKQAFLAELIALRARRRRIACFDGRVYMLNR"
     gene            complement(51674..51934)
                     /gene="rpsT"
                     /locus_tag="PKM94_01350"
     CDS             complement(51674..51934)
                     /gene="rpsT"
                     /locus_tag="PKM94_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD65312.1"
                     /translation="MPHHKSNFKRMRQSEKQRDRNRAWRSHLRGAIRDLRATEPGAPD
                     LAGKYRTVVSMIDRAASRRLLHRRTADRYKSRLARHAASKPA"
     gene            51999..53582
                     /gene="murJ"
                     /locus_tag="PKM94_01355"
     CDS             51999..53582
                     /gene="murJ"
                     /locus_tag="PKM94_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOD65313.1"
                     /translation="MSTPEAKKSSLLGSATTVSAWTAFSRVLGLVREQVMAFIFGAGM
                     ATDAYVTAFRIPNLLRDMFAEGALSSAFVPTFKERLVAGSKDEAFHLANVVMTAILVV
                     VGLVVLLGIVAAPLLIYVFAHGFTADAVKFDLTVSMTRIMMAYLLLVSLSALVMGMLN
                     SFGRFGLPALSPAMFNLGTIVVVLAFYKVMDPPIYTMAIGVLVGGLGQVAIQLPALLR
                     IGYRFRFTISLFDAGLRRVMRLLTPMIIGLSAGRINILVSTLIASFLAEGAISYLNYS
                     YRLMHFPLGVFAVAIGTVALPQVAELVARRQHDRVGDTFLNAVGLNSFVVIPSAVFLA
                     LMGRPLVDLVYQWGAFSSLDSEQTALALLHYSYGLVGFAVVRVVVPFYYAYEDSRLPM
                     KISILAVVVNLVLYAPLVWLLGFAGLAAATSLAALLNAGLLIWFLPRKGVAVSFGRLA
                     FHTARITVIALVAFYVARVIPYDAVIALDDGLPQRTLAVILPALVGAVLYVAFSLALR
                     TREITLLRNRLVRRRRAGN"
     gene            53582..55834
                     /locus_tag="PKM94_01360"
     CDS             53582..55834
                     /locus_tag="PKM94_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOD65314.1"
                     /translation="MARAYPGIFLLIALAGGILAADFLRASAPAFLLAALGGLVAAAA
                     ALVFRRPSAAAVFFGLVLAGLSAAHFARDTYDLGSRSLGRYADSRTRYRLVGRVDDWP
                     ELRPDRIELTVRLDSLGPDRMAAVEGRLLLRIADTGAAVQFGDRVELSTRLYPAAERG
                     EGAASRRSLLVKGVGALAYVRASQDVRRLPRPAVGLIPVVDTVRAAIGQSLRRTLSPR
                     AAALAGGFLIGDTRDIPPDLYRAFRDSGTLHVLAVSGSNVAVVVGFVLVLLRPFRTGR
                     VARTLILLVVVVFFALLCRGEPSVVRASVMAALILLARLAGRRIDLNNLVALAAAAIL
                     LCAPAQLFSVGFQLSFATAWGLVFVTPRVYRLLEGRALGPPARWLSLAVIVSLTAQLF
                     SAPLVACYFHRVPLLGPAANLVIVPLVSAAVVGVLATLTLDFVWPPLGALAGALVDRL
                     MDLILGALGLFSGERAPVLDTGALPPAAAVAAYAALIVAGFALGSLPVRRRAVLGGLV
                     LLNGALAWLALGPRPPRVVCDIHITRVPGGVAAAIVPAGAERADLVITNLAARDYPVV
                     DRVLIPWLERLGRPGLGRVVLLSADYAGAAELARFADSLSADTLFVHGRLHGTMGDIA
                     ARSGRCPPWRPFGGAEPPAGEGLALCAWGAVLAARGLEILFLRTDGDLAAARCAPDRA
                     CWLVCGDEAAADDLRSIGRPPAGLVCAEFAQTEPDSVPAAVFDLRRFGSVRLRLTAGA
                     APSVRVETGR"
     gene            55944..57146
                     /locus_tag="PKM94_01365"
     CDS             55944..57146
                     /locus_tag="PKM94_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOD65315.1"
                     /translation="MSLSGNLKTVSFPDILQLLATGKKTGLLEIRTASRQKEVAFRDG
                     NIIHASSVNSAEDLLGQMLLNRGRLSKKDLDRAITLHKQTGRQLGTTLIDMGVFDKHE
                     IAECLKQQIEEIVYNLFSWKEGEFLFHENAHPKSAPFLVELSTMSVIMEGTRRIDEWL
                     EIQKVLPPDDVLLAISRSPRTNEEEFKISVDEFRLIGLINGERTLPDLINLSPMGEFV
                     TCRAIYRLIVNHLIEAVGRREQKNQVEEDEEEAVLRIVFRLYNNAFYRIRTLFEEYLG
                     EDNTTFATFAAQYRGNLLNFFPGVDPKSDLMPSFDKFLAAVQAIPAPIRFQTLFRDLD
                     DALAEQLQFIFELLGVGAYRDGVTTVKGEISEPLATRRDLVKRYAIEENFNQAVKRSA
                     RVVKMVRG"
     gene            57149..57517
                     /locus_tag="PKM94_01370"
     CDS             57149..57517
                     /locus_tag="PKM94_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65316.1"
                     /translation="MKRTIMSALLLAGALVPFSRAEAANIAAIINPPTFLNLLVLVGA
                     VVCTAGAVQVFSLVRGGRLSRCWQWFIGGFAVLALAEIILLCGLFELLTLPEFVVPTL
                     LLVMAGLFLYGVREIKRTLS"
     gene            57623..58471
                     /locus_tag="PKM94_01375"
     CDS             57623..58471
                     /locus_tag="PKM94_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65317.1"
                     /translation="MNSTTELDERISKCRKILEEDPNSQIFAALAESYRKKGELDKAF
                     RVCQNGLKVHPSYGSAHVVMAKINLDRGLYDWAEVEANKAMELDGRTRTVELLLAEIY
                     IYKGEFERAVKLLSQLHQADPGNAQIERLLEIARKLPEESRAVTEPAAPAPTAAPGPT
                     AAEPDLPLPLAVPQILDEARTIPGVPGALFVNFEGLVIETRWQLDMDQARCGATIGEL
                     GNVLNESLLEGSFGAVRSILVETAGPTFYVIREQDGVFIFVGDESCNLGTLRMRIEKL
                     VARYQP"
     gene            58488..59480
                     /locus_tag="PKM94_01380"
     CDS             58488..59480
                     /locus_tag="PKM94_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65318.1"
                     /translation="MDRNGRYSRPIVLLSLIVLIIPMVLFPNRLGTDLARASLIYLMY
                     ELAWYLAAVFALTRRTSLLPLAQAAGICLLYRLALGLAFGLMIAVAYAMNLRVSLTLA
                     LSSYLPAVLLHVAAAPFILIPVVRRVVAPVHPWPVLVTDRQEGDSPMHDVKKTHTAGS
                     PRGLAAASAAPAEWRSDPERHGRSESEPAPMRPGDANGFDRATSYIGENPAVRAAAVV
                     DIEGLLLGHFQRGGDLAEDWAPLARLFYEHNRDVAARFSGGLPQRLALSFPDRKIIIA
                     REETFYLMVVSEHHHDELLNVRFSQGLEIVRKYVAERYGQERAVNAENVYVRNS"
     gene            59464..59805
                     /locus_tag="PKM94_01385"
     CDS             59464..59805
                     /locus_tag="PKM94_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOD65319.1"
                     /translation="MYEILSELNKTSGVTGSMVVGSDGILIAADLDASFEGETVGALA
                     ASITSNIQKSLHRLDGHPLRQVTIESEEAKLFFTDAGLGILVVTTEKDVNVGLIRLEI
                     KNAIERLRTVR"
     gene            59844..60419
                     /locus_tag="PKM94_01390"
                     /pseudo
     CDS             59844..60419
                     /locus_tag="PKM94_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            60869..61366
                     /locus_tag="PKM94_01395"
     CDS             60869..61366
                     /locus_tag="PKM94_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOD65320.1"
                     /translation="MSDDSLIIYEEEINQIDALISKMLKGAEAKCALLVDKDGHLITR
                     QGFTHSLDTTALAALLAGSFASTKEIAKLVGEPEFSVLFHQGKKDHIHMSLVGERSIL
                     VVIFDDRTTIGMVRLYAKETSAELAKIFDTIKATAAENKQQQAKFANEFAQGAQDKLD
                     DIFQD"
     gene            61452..63149
                     /gene="pilB"
                     /locus_tag="PKM94_01400"
     CDS             61452..63149
                     /gene="pilB"
                     /locus_tag="PKM94_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOD65321.1"
                     /translation="MPSDIGAMLVKAGRITPEQAEKADRMAREHGEKLEAALVRIGAV
                     ESEEEIAKFIGKHLKIGAIRLEDIELNPDIVKLIPVDIARKFKVIAVSKLNKTLLVAI
                     SDPNNIYVLDAVKFITGCIVQPVLSPERAIEKAIDQYYADGGGLSEIVKGLEDAEGLE
                     VVEAEDGPSDMDLMSAIQDKPLVKLVDSIIGDAIRMGASDIHIEMYEKRIRVRYRIDG
                     DLREMAPLPFKYRAAIVSRVKIMADLDISERRLPQDGRIKIKISGRTVDLRVSVLPTI
                     FGEKVVMRILDPNALMVDMTRLGFREEDLAKFDKAIHLPYGIVLVTGPTGSGKTTTLY
                     SALKQINTVDVNIMTAEDPVEFNFDGINQVAVKSDIGMTFAAALRSFLRQDPDIIMVG
                     EIRDGETAEIAIRAALTGHLVFSTLHTNDAPSSINRLIDMGVPYYLVASATKLILAQR
                     MMRKICQNCKEEINLSPEQVESLGAPKELLRNIRAFEGRGCRECGDTGSAGRTAVFEV
                     MPITPEIERLILNRAPDTDIAQKALEQGMFSLRMSAVEKMKAGLVSIDEVFAVTTNT"
     gene            63295..64371
                     /locus_tag="PKM94_01405"
     CDS             63295..64371
                     /locus_tag="PKM94_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOD65322.1"
                     /translation="MFTLRELLEEMVKLNASDLHLTVGSPPVVRVDGRLQRLNYDVLT
                     SEQTKKLCYSMLTEKQKLKFEQNSELDFSFGIEQMSRFRCNVFMQRGNIAVVLRQIPY
                     KIRTFDELGLPKVIAEFAKLPRGLVLVTGPTGSGKSTTLAAIIDKINRERPVHIITVE
                     DPIEYLHRHQAALVNQREVYSDTTSFATALKYALREDPDVVLVGEMRDLETIEAALSI
                     SETGHLAFATLHTNSASESINRIVDSFPTNQQEQVRVSLSFSLQAIVSQALIPKIGGG
                     RVMALEIMVCTPAIRALVRDDKVHQMYSMIQSGQKYGMKTMNQSLSELYLTGKITLND
                     AMSYSSNTQELGEMLSRQKSPAFS"
     gene            64398..65597
                     /locus_tag="PKM94_01410"
     CDS             64398..65597
                     /locus_tag="PKM94_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD65323.1"
                     /translation="MPVFEYKGKTLAGAAVQGSLKAKSKGDLERVLRQNRILVTSISK
                     RAPEISLRIGTGIKRIHVSRFTRQFATMIGAGLPMVQCLEILAAQSENRELARIINDV
                     KEGVSGGATLSEAMARHPKIFDPLYTNMVEAGEVGGALDSILVRLAVYREKADKLIRK
                     VKGAMVYPSVVMIVAIGVTTAMLTFIVPVFAKMFEGLGSELPAPTKIVLHISHFLQAN
                     FVFLTIGLIGLVAFILWWRKTPQGGLVWDKSMLRMPILGTLVRKSSVARFTRTLGTLL
                     SSGVSILDAMDITARTAGNRVIANAINKSVMAIAEGDTITGPLKESGVFPPMVTQMIS
                     VGEKTGGLDEMLNKIAEFYDEEVDDAVSALTSIIEPVIIVFMGIVIGGILIAMYLPMF
                     DIIGKIG"
     gene            65694..67400
                     /locus_tag="PKM94_01415"
     CDS             65694..67400
                     /locus_tag="PKM94_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65324.1"
                     /translation="MVSAEHPRRLAWFIPLRLATFLVVFFVVVFWMKFPGFLALPFFL
                     YAGVTLGFTLLLALDKQQRLGAVTTAMVALHFLLEIGVESGIIYATGNVNSPFSALFV
                     LTIVSAALVYRLVGTLVIASTVALAYAFIVWLGLTNSDPEWSLRALRTVFDTQETAFY
                     SIFLHLLIFYLVAFIAGYLGQRLRDQDRRLADASRALRRARLETDDILRHLNSGLITI
                     DAEGCIVYFNRTAERILGYCEEDIKGLSCDQAFADRMPALARSLMDGVRRRVAHLRQE
                     LEIINPLGRRVPLGLSISILTEEDKATPRGVIAIFSDLTEAKDLEAKVRAADRLAAVG
                     ELSASIAHEIRNPLAAISGSVEVLKSALPVAGENARLMDLIVKESDRLTKILSEFLLY
                     ARIDRPSYTKVDLCRLTSDVLHILSHHESRHPGIRLAFDADQSVVYVAGDEDLLTQVL
                     LNLGVNACEAVGAAPGDVHFRVFSRDPERVVLQVADTGCGMAPEVMRRMFQPFFSTKR
                     QGTGLGLAIVHRVCTALKIGLAVDSAPGRGATVTLEFPVCMPRPQLQGAATASAAPAA
                     VR"
     gene            67491..69257
                     /locus_tag="PKM94_01420"
     CDS             67491..69257
                     /locus_tag="PKM94_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD65325.1"
                     /translation="MALRTYARLTGVLALVIAACGEPPTLTHESVDRFDRVVARVDTV
                     YTLTFPEFYRKLEESILLPDGGRLSQREVADELDRVVMDTLAGLVADTVNLGANYELN
                     RIYRQRYYEYLLRRFGQVAFFEKTSVDSAAVRQYYEQNLAQFTTPEEVDVSHILIAAG
                     NLPHDRDSLKYRGLTQPELAAAAEKHAWYVKSLLDQGHPFDSIAREYSHDNLIASYGP
                     RIGWVRRGVYEHPFDSVAFGLAPGRYSDPYFASNSWHLIYVAAYHSDTTAPLEGTIAD
                     QVTVHLRTAAADSLARPFLDSLDAAMQLEINEAILDSNVFLLPKPLWAAVVNGRDTVD
                     MADLQSLEEGFRKRHRVDNTTPDMKREMIQIAARRYALVQAARAAGIEELPDVREQEF
                     KLRRQYQKTIVLRQGLDDGWTPSDAEIEAYYRRNYRETPIDKPLVVQHIIAPDSASAA
                     FLRDQALAGHDFLALAEQYYPGDSSLRRDLADLGPIGPQDVSPEFYEAARVTPDGNVS
                     FPVQTQYGWHVIKVLEHRTPITLDKVRTQIRQTLRDQHLREITTTFRDRLYRRFAVSF
                     PNRLPAVDLRPIRDRQEGQDGQ"
     gene            69247..69888
                     /locus_tag="PKM94_01425"
     CDS             69247..69888
                     /locus_tag="PKM94_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOD65326.1"
                     /translation="MDSDVTVSAPPSAPPPISFRSELARKATHMFALVIPAGYYVLGL
                     GRGTMLAVMIPVALCTFFVDLARLQGRPLWRDIFGPLFGRMIRAHEAAGDFTGATYIL
                     ASTVATVALFPKAVAVAALAFTVVGDSLAAVIGRRFGRRRFLHGKSVEGSLACLAGTV
                     IVALLTPNLAAPAAFAGALAATVVEALPLQIDDNVTIPLLSGLVMTLVLRILG"
     gene            69997..70203
                     /locus_tag="PKM94_01430"
     CDS             69997..70203
                     /locus_tag="PKM94_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOD65327.1"
                     /translation="MSKGTVKYFNDLKGWGIISSADSPEDIYVHYTSINMNGFKTLRQ
                     GQEVLFDLIHSNGRPLAQNVLPSR"
     gene            70311..71342
                     /locus_tag="PKM94_01435"
     CDS             70311..71342
                     /locus_tag="PKM94_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOD65328.1"
                     /translation="MRKIIDLRSDTVTRPVPAMRAAMAAAEVGDDVFGDDPTVNELQR
                     TVADLFGKEAALYVPSGTMGNQIALKAQTRRGWELLCDRECHIVNYEVAGPVVHSGLL
                     VNLIPTEYGLLTAEVVRSHIRPVNLHTPRTKVVALENTHNRHGGTILPQDEILRVREV
                     CDEFGLVFHLDGARIWHVHVATGRPLAELVAPFDSVSVCLSKGLGAPVGSLVLGSAAL
                     IDVCRRERKLFGGGMRQAGIVAAGGLYAVRHHIGRLAEDHANARYLAAALNEFKLFSV
                     DLARVQTNIVLADINGAETSDSVLARMNEVGVWAIAFGLKRIRLVTHLDVPRADCEEA
                     VARLRTILV"
     gene            71339..72121
                     /locus_tag="PKM94_01440"
     CDS             71339..72121
                     /locus_tag="PKM94_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD65329.1"
                     /translation="MTAPRPADKLTILGTSAGMPQPDRAGSGCLLTVNGRHSLLDCGG
                     GICASFLRRGCDPLAVDRVFVTHTHPDHCTELPLFLQMLHLAGRTDPVELFVPDEFVE
                     PLRALLAAMYIPREKLPFEPRLCGYSDGFRYSGDFTLTARANDHLVHNRELFARLGLP
                     NRMQSHSFDIAVGERRLVYSGDLKAFEEIRPWVDGCACVLIEATHVDLPPLVEFARSA
                     AVGRWIFTHVQSAAHVRDLHAALQAAGLENYVVAVDGMEFPL"
BASE COUNT        12427 a        25190 c        21962 g        12764 t
ORIGIN      
        1 gatcaccttc tcggtgatgt cctcgaccgc catgatgacg ccgtccttcc cgtgggggag
       61 ctgggagatg ggcgagatct tgatcgagag cgccttctcc aggtagccgg tgttgtggaa
      121 gtagcgggag tcggaaaagt cctcctgggt agccagagcc gtgttgatca tgtgcgccca
      181 gcgggtgacc tcggtctcct ggagcagatc gagaaagcgg gtcggtttgt cgcccgctcc
      241 gaccaggacg atcttgtcgc cgttgaaatc gaaaatctcg agtgccgcgg tgttgatcgt
      301 cgtgatgtgg ccgtcggagt ccagcaccac cgtgcccatg ggcgccttgg cgatcaccga
      361 ctcgttgtag cgctgcaggt tcaaaagccg gtcgtagatg ttggcgttgt gcagggcgtt
      421 ggacaccagt tgcgcataga gctcgaacag gtagaggtcc gacttgagga acatccggct
      481 ctcgctcgaa ttgtccaggt agattactcc gaccacctgc tccttgactt tcagcggcac
      541 gcacatgatg gagcgcaggt gcagttcgac aaccgagcgc tggttggcat agcgctcgtc
      601 ggagagcgca tcggacgtgt agatcgactt gcccgtctcg gccacgcggg aggtgaccga
      661 gttggagact ttgaaatcct cgtccatcat ctcgtcgcgg cagagattgt aggcggaccg
      721 gacctggagc tggttggcgt cgtccagcag catcaccagc ccccgctccg cctgcatcag
      781 ctcgatcgct ttgcgcatga cgatctgcag cacgtcgtcg gtcaccagcg acgagttgat
      841 ggccagggag acctccagga gcgccttgag gtcctccacg ctcttggacg tgtgcgtttc
      901 ttcggacgtc aggcgcggat tggcctcggc cacctcgtcc agcgtggcct ccaggtccgc
      961 caggacatcg agcggttcca cgtgctcagc gcccgctccg acctggcctc cgaccccggt
     1021 catgcgaatc ttggctgtct tctctatctg ggcctggttc atgcgcgtac gttctccagg
     1081 gtataacctt tttccactat atcggaaaat ccgtcaaggg attaacccgt gggcggacgc
     1141 ccggaacgcg cagacggcgc tcagcggcgc gggaggactt cgctgagcga aaaggaccgc
     1201 gaggtagtgc tccactcctc gcggccgtaa ttcgtcagcc tcaggaactc gcggaacagg
     1261 gttttcaccg gcgagacgct cccggtgtcg tcatcgacag ccaagttgat gccggtcaaa
     1321 gcaatacggg atatatctaa tgtgatacga taatctcggt cggcgggcag cccggccagc
     1381 gccgctaccc tgaccaccac cgagtccgcc agaaagccct ccgcctcctc ccgcgacagc
     1441 aaaagccgct ccttcgccga ctggccgggc ccggaggtct gcaccctgaa agcttctgtc
     1501 aggagccggt aggagagccg gatcgtgcag gagttcttag tcaccgggac ggccccgaag
     1561 agtttgcggg gccgttggag ttcgatcgca atctcgcagg cgtagtcgat gccatcctgc
     1621 agcgaccggt attcggggtg gccgaagaag ggagtcagat tcagccagac agcgagcgag
     1681 tcatcggcgc ggacgatgtc gtactcgacg ccgccggggg gatcggcccc gcgggccggg
     1741 gcggcggccg ccaggatcag cgccaggagg gccgccggcg accgcctccg tgcctgtggc
     1801 cggaaaaacc gcatctaaaa tacaaagtcc agccgcacgt tcactaccgg gtcgttgtcg
     1861 aagctgcggt cgagccgccg cgccaggctg acccggaaat cggcccccac ggtgagagcc
     1921 gccccgattg actgcttgat gtcgaccgct gaatcgagct tcgtcccgtc ggcgatctgg
     1981 ccgatgtccc agaacaccga cgcgccgata tcggagcggg ggaattcgaa acggtactcc
     2041 gcgttcccca tccagaatcg cgtcccggcg tactccttga agtcatagcc gtggagcgtt
     2101 ccgagcccgc cgagatagaa ccgtttgtgc atcggcagga aaccgtcgga attgccgtgc
     2161 atagcccgca gcagcaacat cccgtggctg tgcactttct ggtagcggcg gagcgtgagc
     2221 gtgtaccggc ggtagtcgaa atcggaactg aaatcgcggt ccgaccattc cagttcggcc
     2281 gtggcatgcc agcccgagaa agcgaacggc tcttcccggt tgtgcgtgtc ccagtcgagc
     2341 acgaacgcca ggcagccgag cgcggtcgtg tcgatgaggg ccgcgccggc gagacgctcg
     2401 gcctcggaca cctggctgaa attctcgcgg aacagcttgc tgccgccgaa gagcgaccag
     2461 aggtggcggt aggagcgccg ccaggttgtc tcctcgtagc ggtagcgcgt ctcgaaccgc
     2521 cactgcggcg ccggcttgac cgaaagccac gccgtggccc cctcggcttc ccagtagtcc
     2581 ctgtaatcct ccgttgccaa cagcgcgaac gcggtattct cctcgtcccc gagcagccgg
     2641 tcatcctcgg aggcgaggcg gcgatagtag gccgcgcccg ccgcgagcgg caccctgcgc
     2701 cagaggggct gctcgatgcc gagttcggtg cgccagcgtt cggactccaa cgcatacccc
     2761 gcccggaacc aggccgaggg cagcagcgag tcggtgtcgt gaaacttcag accgagatac
     2821 ggcgtggcgc cgtcgacccg gttgtacacg aaggcgccga gaaagtcgaa attctcctcc
     2881 cggtagaagc gggggcgccg gcgttcgaaa tactcgcccc ccgagtaaag ctccccgtag
     2941 accgatccct tgtcggccac cgtgatcgcg ccgctgatgc cggcgatatc gccgcgggcc
     3001 gtggcccccg gaccgacgta cacgtcgccg aacagcgaga tcacatcctt gttcacctcg
     3061 ccgagaacct cgatgtcccc cgtgatcgac agcacgagcc cccggacaaa ctgctggttg
     3121 ccgaccgtga tcggctcgct gaacgtcatg aggttcccgc ggcgcagccg cccgagacgc
     3181 tccgcatcgc cggtgaccgt gagaaaatcg atcgtcaccc gcgttggact gtcgatgatc
     3241 cgcgtttccg caatggcgtc gtacgggcag aggcgatcgc ggaggtgcag accggcccgg
     3301 tcaaaccacg ccactcggtc aatgagaatg accgagtcgg gatagacgcg cacggccgcc
     3361 cgggggaccg atgcctcggc cggcaggctg tcggccgccg ccgcccccga gatttcatcc
     3421 agcgtgaaag cgacgcggaa aagcggctcc ggagcagtcg cgcgctggta ggtggtgtac
     3481 gacggttccg gcccgggttg ggctgtggcc ggcggcgccc acagggcgca cgcggctgcg
     3541 gcgccggtga ttgtcccgaa gagcacgaat ttctgcatgc gatcctccgt ttttcccctg
     3601 ccataaaaca aattccgtgc cgcccaggca agcttgttga tcgtcaggca cttgccaatt
     3661 ttccgggatt ggcggccgtg gccgtccgac acaggggcgc ccacttcctg tgcgcgtcac
     3721 ttcaacccga gatccttgat acggcgagac aggttggccc ggtcgacctt cagctcccgc
     3781 gccgccgcgg cgatgttccc gcccgcctcc cgcagggcct gccgaatgag ttcccgctcg
     3841 taggccgcca cccgctcagc cagcgacacg gacgcgcttc ccggcaggcc ggtcagcggc
     3901 gacagctcgg caatgtcggc cggttccacc cggcgcccgg cgcacacgat cgtgagccgc
     3961 tcgatcagat tccgcagttg ccgcacgttg cccgggtaat cgagcgtggc caacagcgcc
     4021 gcgctctcgg gggacaggcg ggtctgtccc gacggatcga gggtgcgcag aaactctccg
     4081 gcgagcagcg ggatatcctc gcgccgctcc cgcagcggcg ggagcgggat gacgaagacc
     4141 gagacgcggt agtaaaggtc ggcccggaat ttcccgtcgg ccaccagggc ggggaggtcc
     4201 cggttggtcg cgccggccac gcgcacgtcg accgtgcgcc gctcctcggc accgagccgt
     4261 tccacctcgc cgctctcgag cacgcggagg agcttggcct ggcactccag cgggaggtcg
     4321 cctatttcat ccaggaagat cgttcccccg tcggccaacg cgaacttgcc ggggaaatcc
     4381 ctcaccgctc ccgtgaacgc accccgcgca tgcccgaaca gctccgactc aaagagcgtc
     4441 ggcggaatgc ccgggcagtt taccttgatg aacggtttgt cgcggcgggg cgacgcgagg
     4501 tagaggcggg tggccgcgag ctccttgccc gtgccgttct cgccggtgaa gaggacggtc
     4561 acgtcggtcg ccgccgcctg gtcgatcgtc cgcagcactt tccgaatcgc cgccgactcg
     4621 ccgatgatcg gcgattgccg gtccagctcg tctatcatga gcgcccgctg ccggtcggcc
     4681 gcggacagca gcagggcggc ccgcaccgcc gtgagcagct tctccggggc gaccggtttc
     4741 tccaggtagt cgagcgcccc aagccgcacc gcctccagcg ccgtcgcgat gtccgactga
     4801 cctgaaatca cgagcgccgg cagccgccgc ccgagccggt tgagatcggc caaccactcg
     4861 acgcccgaac ggccgggcag gttgaggtca agcagtacga ggtcgtagcg ggcgctgtcc
     4921 acccgccgct gcgcctcttc agccgaggac gcggtgtcgg cccggtaccc ctcgccctgc
     4981 agcagcgagg caaaagcggc cgtgatgtgc ggctcatcat cgaccacgag gatgcgctca
     5041 cgcatcatgc ggctccccgg cgtgcggcag tacgatcgtg acggtcgtgc cccggttctc
     5101 gaccgaccgg acctcgagcc gcccgccgtg ttcgctcacc gtcttctcca cgatcgccag
     5161 tcccagcccg gtgccgtgcg ggcgcgtggt gaaatagggc agccgcgcct cacgcgccac
     5221 ctccgctgac atcccccgac cggcgtcctc gaccgcgata accgcgccat cgggccccgc
     5281 ctccaggcgc agactgatcc gcccgcccgg cggcgtggcc tcgatcgcat tctggagcag
     5341 attgtggata gcctcgcgga aataggtcgg gtcgatcgcc gccgcgaccg gctcctccgg
     5401 aagctccagc gtgaacgcga acccggccag cttcccgcgg tagaggtcgg cgaccgacgt
     5461 caggtgcgcc ctcagatcga cggtctccag cctgggggcc gggagcttgg ccagtcgcga
     5521 gaagcggtcg gccagtacgg tcaggtgctg caactcctcg gccaccgcgt ccagcggtcg
     5581 ctgcaccagt tcgaacgagg gcgtgccgct gagcgctttg cggatctggt agacggagac
     5641 cgtgatcggc tggagcgggt ttatcagttc gtgggcgaag cgccgcgcga catgctgcca
     5701 ggcggccacc cgctcggcct gaacgagccg ggccgtggcg gaggccaatt gctgcgacat
     5761 cgcgttgaac tggccgatca gggcgcggat ctcgccgaca ccctgctcgg ccaccgtgat
     5821 gccgaactgc cccgcggcga tctgccgggc cgcccggtcg agttccgcca ccggccgggc
     5881 gagatggcgc gccaggcgcg aggaaaagac gtaggcgagc cccacggtcg cggcggcgac
     5941 cagaccgaac agcagcgcga gaaacaccgc gtaacgcgtg tgcaggccgg gcgccgccga
     6001 ctcgtccgcc cgcaccccgc ggtaactctc cagcagggcc gcgaactccg gagaatgggc
     6061 gagcccgccg acgagcagcg tgtcgccccg acgaattgag gcgtaccggt acaaccgctc
     6121 cccgaccggc gccgtcccgt ggtcgcgtcc gctcgccacc gccctggcca ccggggcggc
     6181 cacctcgtgc acgacctctc gctgcgtcca caccgactgc accttgccgc ccgcaaagag
     6241 catgaaaaag ccctgggatt cgagaatctc ccgggcgttg ggttgcccga gaatcgccgt
     6301 aatcgtgctg tccaggaggg ccccgtactg ctcgtggagg cgcgcgacga cggcatcgtc
     6361 ggccggcccg cgctccaggc ccggctcgac cgccaggtag tagccgaccg tgacgagcac
     6421 aaccgcgggg accagcgcga acagcagaaa gaggcgaaac aggcgtgagc cgaaagccga
     6481 atgcacgact cacctcctgc gcacacggcg gtcgtagtcg gccagagcct gccggtggga
     6541 ttcgaacgct atccggtccg ggagatcgtc gaacgaaaag aagcgcgcct ccgcggcgtc
     6601 gtccgccgga cgcagcgcgc cgccgatccg ctcggccagg tagagcagga gcacggcgtt
     6661 cacccgcggg tcgtcggtcc ccgagtagac ctcgaaaaag ccggtgaggc ggacgatcag
     6721 tcccgtctct tcggccactt cccggacggc ggtctgggcg ggatgttcgc cccattccat
     6781 gaacccggcg gggaagcacc accagtccac catgggcgga tgcgcccgcc ggaccaggag
     6841 cacgcggtcg tcctcgacga cgatgccccc ggcagccggg atgggattct ggtagaacac
     6901 gaatccgcac gcagagtcgg ggcaggccag gcggctgacg ccgtcgatcg tggccgccgc
     6961 cagggggcgg gcgcagcgcg ggcagaaccg gtacccgcag tggcgggcct tgagatccct
     7021 cagccgcgcg tcgtcgaagt catgatactt cttgtccatg cgattccctg gaggcttcgg
     7081 ccgcccggag gtgattcgat ttcatcgtga tgatcgtggt gtcgtcgcgc ggcgggaagg
     7141 tcgcgtcgaa agcccgcacg tcctcgagga tggcctcgag gatttcaccc gcctcccggt
     7201 ggcggtgttc gcggacaaag cgccgtacgc gctcctcgcc gtactcccgg ccgtcgccgt
     7261 ccatcgcctc cgacagcccg tcggtgaaca ggaagaggac gtcgccggga cggaactgca
     7321 cgtcggccga ttcgtaccgc atctcgggga aagcgccgat gatcgggcct ccctggacga
     7381 gcagttcgac cgcgccgtcg ctccgcacca gcaccgggta gttgtggccg gcgttgcagt
     7441 actcgagccg ccccgtccgc cggttcagct cgccgtagaa gagcgtcacg tatttctccg
     7501 aggaggtggc ggccaccacc tggtcgttca ggtgcgtcat gatagccccg atcgggttgc
     7561 cgttgttgac ctcgctgcga atgatcgcct ggatctgcgc gatcatcagc gccgccggca
     7621 tccccttgcc cgaagcgtcg gcgatcacca tccctatccg cgcgtcgtca atggggatga
     7681 agtcgtagaa gtcgcccccg accgtccgcg agggcgtcga atggacggcg atctcgcaca
     7741 gctcgagctt ggggggcgcc gcgggcagca ggtcgagctg gatctgccgc gccaggctca
     7801 cttcctcctc caggcgaagc cgctcgagcg aatcgacata gagccgggcg ttggtcagcg
     7861 ccgtgaccat ctggttcgac aacaccccca gcaggttcag gtcctcggag gagtaccggt
     7921 acccggcgac cttgtcggtc agggccagga acccgagcat gtgcttggcg tccttcatcg
     7981 gcaggacgag cttcacctgc cgctcggtca ggagttcagc cagcggcgag ttcaccaggt
     8041 agtcgccgag cgtctggaga aaggcggggg agtccaggag gttgatcccg cgcaggagaa
     8101 ggtcgtcgcg gctgagcacg atcctccgcg gctggtcctg gcttcgggtc agcgcgtact
     8161 ccccgacctc atcgtcgaac agcacgaagt agacgcgctc gaccaggagc gccgtcttga
     8221 gcgtgtcctc aatgatcccc cggagcttga ccgggtcgaa cagcgaaatc acttggcgcg
     8281 agaagcgctc gatgatgttc cggtgatcgg tgcgcgtgcg gatgaagagc gtgcggatga
     8341 ggttgtcgat cgcgttgttg atcggctgga acagcagcag gatgacgatg atgaaggcgt
     8401 agctcaccac ctgcgcctgg gcgccgaaca gcggcgtgaa gaacgagcgc gactccacca
     8461 ccagcagaat gtacagcccg acgagcaggc cggaggtgat cgtgtacacg aacgactggc
     8521 ggaaccgcaa ctgcacatcg aggaactgga agcggatggt ggcgtaggcc aaaaagaccg
     8581 cgcccaccag cgcccccgcg accaccagca ccgcctggac atcatccgaa aacaagcggg
     8641 gcaaaagcag gccgcccgca aaggcaatca cgtacggcgc gaggccgatc cgcatcccgc
     8701 ggaacaacac ccgcgtctgc gtcagcagac ggggattggt gaccatccgg cggcccgatt
     8761 ccaggaagta gacggccacc acgacgtaga gggcgttgag cgccccgaag atctgctggt
     8821 cataggcgcg gatcgtgttg atcgaaagga gcaagaaaga cagcactttg gccagcggat
     8881 tgaggatgat gctcacaaac cccgaatccc ctggctcgat ctggaggaaa cgcaggaccc
     8941 ccgtgaactg gttgtacagc aaaacgagcg cgagatggag cgccggcgga gcgaaaatca
     9001 ggtaccgcag acggttgtgc ttgaactccc gcagacggtc ctccgggaaa atccaggcga
     9061 agagcaaaag cagggggaag aagaactccc agaggtgctg gaggtggtag agggtcgatt
     9121 cctcgaagcc cagggcggtg tcgggggaaa gccgcacgat cgcgccgagg gctaaaaaga
     9181 gcggccccag gccggcgaaa aacagcatcg tggccgtgac ccggttgagc cgattggcga
     9241 agttgtcccg gacgatggtc accgccagga agacgagaaa gccccccgtc agcaagaaca
     9301 ggactatctg taccaactcg aagttcatag gtcagactcg cgccagaccg cgtcgcgtca
     9361 cctgatcgtc ttggtgattg tcaccgacgt gccgcccgcg ctcacgtcga tatccacgct
     9421 atccatcagc gaccggacga tgaaaatacc ccgcccgacc tctttgagga ggttttcctc
     9481 cgccagggga tcgctgacct ggtcggggtc aaacccctgc ccctggtccg acacccgtat
     9541 ctgcgccgcg ctgtcggacc gcgtcacctc gatcgagatc tgctgctcgg ggttgacgcg
     9601 gcccccgtgc acaatggcgt tgttgaccag ctccgagacc gagatggcga tatcggcgat
     9661 caccgattcg tccgttcccc agccgcgcag gattccctcc acgaactcgt cgacagtcgg
     9721 caggtagtcg tggctggagg ggacgacgat ggtgtttccc ttgatgacgg gtcgtttcat
     9781 acttgtgctg tctccaaaaa aacaggcaga gccgggcagg tctgcctgtc atgatgattt
     9841 ccgcgcctgg gctgtccgct gcgtctccgg ctatcggaac gaccgcaccg cgtcgtcgac
     9901 cgagtcgtac gcatcaaaca ccgtcaccag tttcgtgatg gtgaggagcg actgtatctt
     9961 gtccgtcaca ttggccagtt tcagttcccc ctggtgattg cgcagggtcg tgtagctgga
    10021 tatcaggatc cccaaccccg tcgaattcat ccagtccact ttgcccaggt cgatgacgac
    10081 ctgtcgtctt ccccgttcga tgatgtcgtg cagtttgtcg tgcagcaggc tggcatcggg
    10141 cccgcccatg attttgccct ttggctccag cacgaccacg ccatcctgct cccggtcggt
    10201 catcttcatg ctgatctcgc ctccgatacg attataggta cgtacgaacc tgtcctgata
    10261 tggtttcctg accccggtgt gcgggacggc cgtagcgtcc ccggggggtc gagcgcgact
    10321 cccgcggggg agcggaaggg tcgaaaaccg ctaactggtt aatgagccga aatttccgag
    10381 gggtgtcaag gggttttgtc gttctccgcg ccccggtcgc gcccgcgggc gccaaagtgc
    10441 gcgggaacgc caagtcctgc ttgactttcg tccaaaatgg gttagcttgt ccatagtaga
    10501 cgcaggccgc cggcgcatcc ggctgaggcg ccggctcacc tttgaaggaa caacttcccg
    10561 gcgccgaagg cacttctccg gcccggttca gtccgaacgt aacaaccata ctatcccacc
    10621 attcggagtg acattatgcg ccccctaacc gccctgttcc tgccaaccgt gctgctgctg
    10681 gcctcggccg tcgcggccgc cgtcccttcg actatgacct tccagggccg cctgaccgat
    10741 gacctcggcc agcccctgga caccaccgtc gcactcacct tccggctcta tgtcgcgagc
    10801 tccggcggct cccccgtatg ggtggagacg cacgacgacg tagtcatttc gtacggcgtc
    10861 ttcgaggtgc aactcggaga gacctccccg ctaacccgct cgcacttcga cggttcgccc
    10921 cgctggttgt cgattgccgt ggacggggga gcggaatcgg ctgtccgttt gccggtgaac
    10981 agctcggcct atacgatccg agccaactac gccgacacct cgggccgggc gaaatacgcc
    11041 gacaccgccg ctttcgcagt aggcgtcggg aactccggct gggcggacga cggagcaaac
    11101 gtgcgactgg tgaccgccag cgactggctc ggcatcggca cggccagtcc ggaagacaag
    11161 gtgcatatcg agagcagcct gagcggggga acggccttcc tgaaacttga gacctcgcat
    11221 gcctctaact ggggggagac gggcctgcgc atcgaaacgc cgcagaaccg ctggcacctt
    11281 cgcatggacg acgccacgaa caacaacatt cccgacggcg ccctcgggct gcggagccag
    11341 gacgtcggca ccgaaatcat gacctggacg agcgacggca aggtggggat tctgaacacg
    11401 ctgccggagt atcccctgga tgtgcgctcg accgcggtcg cgatttacgg tcgcacgacc
    11461 gggggcctcg ggacggccgg cgtgtacgga cgggcggaga acgacggatt cggggtgatg
    11521 gggacgtcga cgagcggccg gggcgtctac ggggcgagcg cgagcggttt cggcggttat
    11581 ttcctgggcc cgaccaccta cgtctccggc aacctcggcg tcgggaccga gacgcccacc
    11641 cacaagttga ccctcaacgg caccctcgct tatcagagcg ccgggacgac caagtatcac
    11701 gtcgcctact acggcggcgg cctcgatttc tgtgaaacgg gcgtcgggga ttaccgcctc
    11761 atgatcgagg acgggggaga ggtggggatc ggaaccgcca acccgaccgc caaactgcac
    11821 gtcatgggga acgtcaaggt gcgggacacc ctcaccgcgg gcgccatctt ctccagcagc
    11881 attgtcgatg agttcggctt ggcgtcggcc aaaaacgccc tttacgaatc gacggcctgc
    11941 accggctcgt ggaaatcatg tctgtcccgg accattcgcg tgcccgcgcc cggctgggtc
    12001 ctggctgtcg cgacggccag agtgaagctg gatcatccgg gctcaggttc cactgatgtc
    12061 agcgtcgctc tgtcggatga ttcgacaacg gccgacgact ggtggtatgc caactgggcg
    12121 ctccccggcg cggccgtggc cgggaactac tactcgactc tcaaccccat gcggctattc
    12181 cgaaccgata ccgccggtgt gtataccttc tacgtgaacg caaggaggtt ggacggggaa
    12241 gcttctgttc tgggcgccca aatgcactgc ttcttcatcg ccaccagcta cagcttcgat
    12301 gaggctgttg cgggcggcga ggggagcggc gcgcagccgg ctgctcccgt attgaccgag
    12361 ctcggggacg ttccggcgcc ggccgaaaac cccggggctg cggacgtcgc atcaatcctc
    12421 gccgagctct ccgccctccg gcaggaagtg gagtcgctca ggaacaaggt cgaggcgaac
    12481 cgatagaacg ggttcactcc gatattgccc caaaacgaga ggcgtcggcc gtttccggcc
    12541 gacgcctccg gcttttcctt agggccgggt tcagccggct tttttggcgg cttgcgcgat
    12601 cggccggtaa tccagcgctt tggtcgggca gaaccgggcg caggccgggt ccccgccgca
    12661 caggtcgcac ttgaccaccc ggtcgtgggt gtgatcgagg atcgcgcacc cgaacgggca
    12721 ggccgccacg caggccatgc acttgacgca cgtctcctga ttcagctcga tcgccccggt
    12781 ctgttcgttg cgccggagcg cgcccacgag gcaggacttg acgcacgcgg ggtcgtcgca
    12841 ctggaggcac gtgaccgtca cccagagctt ctcgtgcccg ccgtcgagca tgaatatccg
    12901 cgaccgcccc ggcttgccgt ccgccgaatg ggtgaacgag caggccagct cgcaggttcg
    12961 gcagccgatg cagagattcg gccgcaccac caaccgggaa ttcatcgcgc cgcctcctcc
    13021 cggttccact gggcgatccg ccccatggtg ccggccagct ccgcgcggtg gcaggcgtcg
    13081 cacacgtcga agtactcccg ggggatctcc cgccgcgcgg cgaggttgtc ggcgaattcg
    13141 cgggtgatgg tcggcgcgcc gcagcgcgtg cacgtgacca tctggaactg cttgccccag
    13201 atttcccgcc cggcgccgaa ttccttgtac gtgataaacc cggtcgggca gatctgggcg
    13261 caggccaggc agccgacgca atccggcggc ggctggtcaa gcggcggcgc cacgcgcttg
    13321 ccgtgcccgc gccccaccgt cgatatggcc cggaaaccca tcgcgtcgca cacccgcgtg
    13381 cacagcgcgc acaaaatgca gtcgtccccg tccggcgcct cctcgtagct cgtcttcaga
    13441 accccgtact cggccgccat ctgctggatg agcggcgtgc gggggtgccg cgcgagctgg
    13501 agatcgagca gcgtccggcg cagttcgatc accgtgtcgg agtgggtgcg cacgatcagc
    13561 ccggcctcga ccggatagag gcaggaggtc acgtagttct tccagccctt ccactcgtcc
    13621 ctggtgatct ccacggtgca gagccggcag gcgccgaacg cctccaccgc ctcgtggttg
    13681 caggtcgccg ggatgtcgat cttctcccgg cgcagcaccg ccagcagcat ctcgcccttc
    13741 tccgcccgca ctgcccgtcc gttgacttgt atcgtaaccg ttgccatgag ctatgccacc
    13801 tccacggcat cccgccggca caccgccata caggcgccgc acttggtaca gatatcctgg
    13861 ttgatcacgt gcaccttccc cttccgcccg gtgatcgcgt tcaccgcgca ggcatggatg
    13921 cacgccatgc agccgtcgca cttctccgcc acaatgtcat agcggatgag ggccgtgcag
    13981 acgcccgccg ggcacttgtg gtcccggatg tgcgcgaggt actcctcgcg gaagtaccgg
    14041 atcgtgctca cgaagggatt gggacccgac ttgccgagcc cgcagagcga gccgatgatg
    14101 atgttttccg agagtttctc catcagggcg aggtcggcct cggtcccgcg gccgtcgctg
    14161 atccggttgg ccagctcgtg cagttggaag agcccctcac ggcagggcgt gcacttgccg
    14221 catgattcct cgacgaggaa gtggaggaag tacttggcca cgtccaccac gcaggtccgc
    14281 tcgtccatga cgatcatccc ccccgatccc atcatcgagt aggcgtcggt caacgtgtcg
    14341 aaatcgaccg gcaggtcgag cttgtcggcc gggaggcagc cgcccgaggg gccgcccgtc
    14401 tgcaccgcct tgaacggccg gtcgtggaga atcccgccgc cgataccgta gatgatctcg
    14461 cgcagcgtcg tccccatcgg cacttcgatc aggccggtgt tgcgcacctt gccgaccagc
    14521 gagaacgctt tcgtcccggt gctctttggc gtgccgatcg cggccagcca ggcgccgccc
    14581 ttggcgatga tcaccgggat gttggcgtag gactcgacgt tgttgagcac tgtcggtttg
    14641 tcgtacagcc ccttgagcac cgaccggacg tacttggccc gcggctcacc gacctcgccg
    14701 gccaccgatt tcatcagggc cgatgattcg ccgcagacga aagcccccgc cccgcggctg
    14761 attttaatgt cgaacgagaa ctccgtgccg aggatgccct cgccgaggag cccgtacgcg
    14821 cgtgcctggg tgatcgcccg ctgcaggttc tgcacggcca gcgggtactc ctcccgcacg
    14881 taaatgtagc cgtctttcga tttcaccgcg aaggcgcaga taatcatccc ctcgagcacg
    14941 ctgtgcggat ccccctccat gatcgagcgg tccatgaacg ccccggggtc gccctcgtcg
    15001 ccgttgcaca cgacgtagtg cggccagtcg tccgccgtgg cgcacgactc ccacttgcgg
    15061 ccggtgtgga aaccggcgcc gccccggccg cgcagccccg aggccttcac ctcggtgatc
    15121 acctggtcgg gcgtcatcgt cgtcagcacc ttcgccagcg cctggtaccc gcccacccgg
    15181 atgtactcgt cgatcgagta ctggtcgatc ttcccgagat ttcgcagcgc gatgcgcgtc
    15241 tgcttgcggt aaaaatcgat ctcgtggtag gtcgtcttgg gctcgccggt ggcctggtcc
    15301 cggtagagca gccgctcgac cgtttggttg ccgcggatcg actgctcgat aatctccggc
    15361 acgtcggcgg gcgtcacccg cgtgtagaac gtcccttgcg gctcgatcac gacgagcggc
    15421 cccttctcac agaatccgtg gcagccggtc tccttgagcg cctcgacccg gaagtccctg
    15481 atccgtttct tctccaattc cgagcggaac gcctgcacga gcttggtggc tccattagcc
    15541 agacagcccg gcccgcagca gatcatgacc ttcttgccaa gtttcttatc ctgcgccgcc
    15601 aggtcggtct gcagcctgct cagttgcgcg ggggaggtga ttttcactcg gccgcctcct
    15661 tcctgtcctt cttcgctccg atcgtgaacc ggcgggcgtc gctcaccttg cagtctccgt
    15721 ggtactcgtc gttgatgatg accaccggcg ccatggcgca cgcgcccacg cagttgacca
    15781 cctcgattgt gaacttcatg tcccgcgtgg tctggccggc cttgatgccc agccgcgtct
    15841 ccaactgctc cagaagctgc ttggcgcccc ggatatggca ggtggtcccc atgcagaccc
    15901 ggatgatctt gtcgccgcgc ggcgtgaggc agaaggcgtt gtagaaggtc gataccgaaa
    15961 agactttcga cagcggcacg tggagcgcct gcgccgccgc cgtcagcgcc tcgcacggca
    16021 ggaaattgta ctccttctgg atatcctgga gcacggcgat gagcgacccc ggttcggccg
    16081 gatacctccc gatgatgtcc ggcagtcttg agaaatcgtg cttcatacgg tgtcctcctg
    16141 gccgacccgg gccgcggcca gccggcggcc ctcctcgaga agcctcaggt tggcctcgag
    16201 cacgctcgcc ttgaacgcca tcttctcgcg cacggtcgcg cgcaccgtgt cgaacgacac
    16261 gattccggtc gcgccgatga gggcgccgag caccgcgatg ttggccgatt tgggggagcc
    16321 ggccgcgatc gccatctcgt tggcgggaac cagcagctgg cggatgtctg tgcggctgct
    16381 ggtcacgtcg atgagcgagc tgttgacgag cacgaggccc cccggccgta cccgcggctc
    16441 gaatttgtcg aacgagggcc ggttgaaagc gcacaccgac cagggcgtcg tcacgatcgg
    16501 agacccgatc cgcgcatccg acaccaccac cgtgcagtag gcggtcccgc cccgcatctc
    16561 cggtccgtag gaggggatcc aggccacgtg tttcccctcc ttgatccccg cgtaggccag
    16621 cagttgcccg gccgtcatga tcccctggcc gccgaacccg gcgaacatca cttcgtactg
    16681 cgccatcacc gcacctccgc cgtggtctcg ggcgtgcgga agcgccggag cggatagtac
    16741 ggcaccatgt tgtcctgcag ccaccgctcc gcttcctccg gcgttttgcc ccagttggtc
    16801 gggcaggtcg agaggacctc gaccagcgag aagcactcgc ccttcacctg gtattcgaaa
    16861 gcccgccgga tcgccttctt ggcggtgagg atgtgccgcg gcgtgtgcac cgccacccgt
    16921 tcgagataca gaggcgtcgc cagcgtcgcc aggagttccg acacccggat cggccagccg
    16981 gtgagcgcaa cgtcgcggcc gttgggcgag gtcgtcgact tctgccccgg cagcgtggtc
    17041 ggcgccatct gtcccccggt catgccgtaa atggcgttgt tgacgaaaat cgtggtgatt
    17101 ttctcccccc ggttggcggc atgaacgatc tccgccattc cgatcgaggc gaggtcgccg
    17161 tccccctggt aggtgaacac gatcaggtcg gggcgcagcc gcttgaaccc ggtggccagc
    17221 gccggcgcgc ggccgtgcgc ggcctcgaga aagtcacagt tgaaatagtt gtaggccagc
    17281 accgagcacc ctactggcgc cacccccacc gtccgctccc gcacccccag ctcatcgatc
    17341 gattccgcca ccaggcggtg gatgatgccg tgggtgcagc cgggacagta gtgggtgact
    17401 gtttcctgga gggctgtcgg ccgccgaaag atggttgtcg tcgcgtgtcc catgccgtcc
    17461 tcacctcttc ttcccggccg ccttcttctg ggcgtccgtc ttcctggcgg cggttttcct
    17521 ggcggcccgg cccgcggctt tcgccgccgg ggcgcccgtc cgcccaagca tcgcgagaat
    17581 cttgtcccgc acctcttccg gcgtcgggac gatcccgccc gtccggccgt agaacgcgac
    17641 cggtttcttc ccctgcatcg ccagctcgac gtcctcgagc atctgcgcag tcgacatctc
    17701 gaccgtcagc acggcctcga tattcttctt ctcgatctcg gcgcggatct ggtcggcggg
    17761 gaaggggaag agggtgatgg ggcggagcag cccgaccgac acgccctccg cctcgagttc
    17821 gtcgattacg gtctggcaga tgcgcgccat cgtcccgtag gacacgatca gaatgcggtt
    17881 cttcccggtc accttgtagc actcgcaccg gacttcttcg cggcgcatgc gcgcgtactt
    17941 ttcggccagg tcccaggagt ttttctcgag cttctccggg tcgaggaaga gcgatttgat
    18001 gatccgcggc cggcggccgc gggcgccggt ggccgcccat tcgtcgttcg gcggcagggg
    18061 atcctcccgg tattcctccg gaaattcgac cggctccatc atctggccga tcatgccgtc
    18121 gccgatcaac atcaccgggt tgcggtactt gtcggcgagg tggaaggcga gcatcatgag
    18181 gtcgacggct tcctggacgc tcgagggggc cagcacgagc acgcggtagt cgccgtggcc
    18241 gccccccttg accgcctgga aatagtccga ttgggcgggg agaattccgc ccaggccggg
    18301 gccgccgcgc atgatgttca cgatcaccgc cggcaggtgg gcccccgcca tgtacgagat
    18361 cgcttcctgc atgagcgaaa tgcccgggct cgacgaggtc gtgaagaccc gcttgccggt
    18421 cgaggccgcg ccgaaaagca tgttgccgac cgccacctcg ctctccccct ggagaaagac
    18481 ccggccgact tccggcattc gcttggccat gtactcggcg acctccgact gcggcgtgat
    18541 cgggtagcag aagtaacctt cggcgcccgc ccggatcgcc gcctcggcga gcgcttcgtt
    18601 tcccttcatc agtatcttcg ccatgatggt gtccgctccg atcctcagta gtccacgagg
    18661 acgaacgtcg ttccgtgggc atgtacttcg atggccgcat cggggcaggt gagagcgcac
    18721 agccggcacc cgatgcagcg ggacggatcg tgcagccgcg cgtagaagta tccccgcacc
    18781 gtgatgtcgt ccgacatcga gaggatcttc tgcgggcacg ctttcgtgca caactcgcat
    18841 cccttgcagt gattcttatc tatgcgtacg cctggcatga gaccggtcct cgcccggtgt
    18901 cagccgcccg gcctgcggct cggccgttcc cacggcttga gcagcagacg gtccagcatg
    18961 agcaccggca catctatctc ttcaggattt atttgcctga cctggtcctc gagcgccgcc
    19021 acgaacgcaa tcggcagact ggtgtcggcc gccagcgccc gggccagccg gagaccctcg
    19081 cggatcgtct cgaccgtcgt gtgctcgagc atgtgcgtgt tggaaatgat cccggtgaac
    19141 gccagccgcg aggtggcctc gatcttggcc atcagcgccc ggcagcccgc cacggtcgcc
    19201 gtaaagggcc ggtgggcgtt cagcaccagc aacagctcgt agtcatcagc cgggaccgcg
    19261 tccgcaagcg acccgagcac ccgcgccccc acgtcgtcgc cgccgacatc aagaatcagc
    19321 gtcccgccaa actgctccat cgccccgcgc acctccggca ggatgatcgg caggtcggcg
    19381 tgggccaggc cgcccgtcgg gtggatcgat tggatgccaa gttcggacaa ctgccgggtc
    19441 gcttcccgcg accggaaata cggattgaca atgtcgaggt cggcgatcgc caccggtttt
    19501 cccccggtca gcgccaactg ccgggcgagg ttgaccgcca cctccgattt cccgctgccg
    19561 taaccgccga ctatggtgag gacccgtttg gcgaacgagg gggaggggta tgtgggggtc
    19621 ggtctggcca tcaacaccgg tgcatccctt cgaggcaatt gcctgggccg gccgacggtg
    19681 gggcgggtgg gcccaatccc cacaaaatcg accttacaaa gccacctgtt gctctcttgc
    19741 gaaaaatatc gccaatggcg ccgggcaagt caagccgatt tgtgaaaata ataacttgat
    19801 tatcggtttg acagttgccg ccgctctcaa tatattgcga ggccgtaaac agttaggtgc
    19861 accgccgacc agcggcatca accggaaggc actcaaaagc gatgcttcgc gccccatttc
    19921 cgtgcatttt tctggcggtc gctctcgggc tcgtcctggc cgcctgccag tccgacaaaa
    19981 aggagacctc accgtccgcc gccgacgatc cctacgccgg ctggaagacc tacaccaacg
    20041 gcaccctccg cgtccattac ccggaggggc atccgatggc ggcccatgtc gaggaaatgg
    20101 cgcacgtgtt tcccgccctg atgcgccgcg acgctcagtt tttccaggtc cccgtcccca
    20161 ccgataccat cgaggtctac ttctacaccg gggtcgggca tgggcataag atcacgggga
    20221 cgtattatcc gtacgccgac accagtgtaa ttcacttttg gctgccgagc aattacggcc
    20281 cgatcatggc tcagctaatg ctcaagcggt ggtatcccgg agagccgaag ttccgttttc
    20341 tgaagcacgg ggtgagggcg cttttggagt ttgccggtgt caactaccac cagcggacgc
    20401 aggagttgat cgattcgggg aagtttgtgt ccctggctga gttagccgcg gacactgcgt
    20461 ctgacgatta ccctgatacc gagtccgaac agatccggcg cgctctcggc gcctcctttg
    20521 ttgactttct cgtggtccac gagggcattc gggctttcgg catgatgtat tcttcaccgc
    20581 tgccgttcga ttcgaccgtc cagcgcatgt tctcggtttc ggccgacagt ctgcagcagc
    20641 tctggctgaa ttttgttcct ctcggcgctc atcctgagga actcgggaga ctgccgcgcg
    20701 aggctccgcc ggccgtccca tgatgggcgc tgtcggcgcc agctcttcgc attctcgaca
    20761 cttaatataa acaaaggtgt gaacgatgcc tgaggaaacc tggtctaccg ccatcactga
    20821 tatcgctccc ggccgcatcc gcgtccgcgg ttacgccatc accgatatca tggagaagct
    20881 ctcctacgcc gaggccgtct tcctcattct gaagggccag ttgccctccc cggcggaggc
    20941 ggagctgatg aacgccgtcc tggtgtcatc gatcgaccat ggcgcctcac cgccctcagt
    21001 cctcggcgcc cggaccgtcc tttccggcgg caactccctc aacgcggccg tggccggggg
    21061 ggttctggtg atcggggaca cgcacggcgg cgctattgag cagtccgcca agctcctgca
    21121 ggagtgggcg gccaagagcg gcgatgtcga gacactcgcc ggcgagctcg ccaactggct
    21181 gaaagtgaac aaaatgcgca tgcccgggtt cggccatcgc ctccataata tcgacccccg
    21241 cacgggcaaa ctcttcgccc tcgccgatcg ccacggctac cgcggacgcc acatcgacct
    21301 ctgcagagcg atcgagaaag cgctggccga aaaactcggc aaagaactgc cgatcaacgt
    21361 cgacggcgcc atcgccgccg tcatctccga catgggcttc gactggcggc tggggaaagg
    21421 gttcttcatc atctcccgcg tccccggcct ggtggctcat gtgtacgagg agatgacgcg
    21481 cgagaagccg atgcgccgtc tcggcccgcc gccgttcaag tacgatggtc cgccggatcg
    21541 ggacatcccg tagtctctcc ggatacgccg cctttccctg tgcgccggcc gagcctgtgg
    21601 gatcggccgg cgcccgagcg catcgagaac cgccccgcga cccgtgttaa atcgttgcct
    21661 ttcagcgcgc cccatcattt acttggattc tgtgaatgtg accgctgata ccgatgaatt
    21721 ccgtcggcgg ctggcctgct ttgtcccgga cgggcacacc cgcgagcggc tactggcgct
    21781 ctgggccgcg gccattgcgc tggccgatga accggcgatg agttcggcgt ttcacctcgc
    21841 cgccggacgc gggctggatc gcgcgtccct gtacgagatc gtcctccagt cgtatttgtt
    21901 tcttgggttt ccccgcatgc tgatcgccgc cgagcatctc caccggcagt ataaagagga
    21961 gaggcccccc gcgtctgtgg aggcggactt tgccgccgag gcctggtttg accggggcac
    22021 ggcgctctgc cgcctcgtct atgccgagaa ctataaccgg cttcgggagc gggtggaagc
    22081 gatggcgccg gaaatattcc ggtggatggt gttggagggc tacgggaaag tgctctcccg
    22141 ccccgggctg acgatcatcg agcgggaact ggcgattatc gcctgcctgg cgatcgagaa
    22201 tcggccggcc cagctgcaca gccacgtgcg gggagcgctc aatgtgggta cggcacccgg
    22261 cctcgttcgg gctgtggtgg aggatatcgg cccggcggcc ggcgagggct accagacggc
    22321 gcgcagcatc tgtcggcgcc tgcggattga actatgatgc ggcggctcag aaaaatcatc
    22381 ctcgtgctcc tggctgtcgc catggccgtc ctcgtcggcg gcgctgtcta cctggtttac
    22441 ctcggcggca tcgagcggct ggccaacgac cgcctccgcc gcatgttcgc ccagcagaac
    22501 aacctcgaca tccggatcgg aaaaatcggc ggcgacatgt tcagccgcct ctcgctgcac
    22561 gacgtggaga tctactatgt cgacagcacg caccgctacc ggctggcccg cttcccggaa
    22621 ctccgcaccg cctactcctt ccgcaacctc tggaaccggc gctacatcct gaatttcctg
    22681 tacatcgact ccctcgacgt cacgctcgtg caggacagcg tcgccggctg gctcctgccc
    22741 ccgctgaccc gcccggcggg agcggccaaa ggcccctccg atctgccgtc gttcctggtt
    22801 tcgcaggtca cggtcgacca cggccgcctc actgtggtcc gcggcggcga caccacgacc
    22861 gtccgggatg tcgccttcgc cgccgccgta gagggcgacc aggggctgta cacggcggat
    22921 ctcgcccgcc tcgctttttc ttccgacctg cccggtgtga agctcgacgc gggccagggg
    22981 aaagtcacct acgccgaccg ccgcctgctc ttcaccggcg tctccgtgct ctccggctcg
    23041 acgcggctga gtctcgaggg gaacgtgcgt ttcgaagacg agccggccgg ggaggtgcgt
    23101 ctgagcggcg atgacgtgga tgtcgccgac ctcgcccgct acctgaacgt ctcgctgccc
    23161 ggcacggtcg atctcgccgg cgaggtctca tttgtcggca gggcggccga gggttcgctg
    23221 aacctcgccg gcagcctgag catcttcgac atgcagaacc tcgtgatcga ctttcactac
    23281 cgcgacaacc ttctcactct cgacagcctc tacggcagcc tcctcggcgg ctgctcgctc
    23341 gacggcagcg gggatatcga cttcgccgcc cggcccaaca cctaccggct ggaggccgac
    23401 atccgcaact tcaacctcga ccgcctcgtc ccgggaacct tcgaatccga cctgaccgga
    23461 cgcatcgccc tggaggggga gtcgttctcc agccgcagcc tccgcctgac caccgtcgtc
    23521 ggcctgtacg aatcccgctt cggcgatttc ccgatccagc aggcctacgg cacgatggtc
    23581 atcaccgccg attcgctggt cttcgtcgat cccttccgcg tcgactactt tgagaacgtt
    23641 atctgggccg gcggcgtcat tgactacgat gacgacatca acctgtcggt ccagatcgat
    23701 ctcaacaacc tcgaccgcta tcggaacacc cggctgttca tcgaggagcc gggcgggcgc
    23761 ggttacgccg aggccactct gaccggccgc accgtcgacc ccgatctccg ggggtggttc
    23821 gcctccgatt cgtgctggat ctacggtctg ttcgccgaca ccctgtacgc cgaggtggat
    23881 ctcgaccggt tcctggccgg ccgccggggc acggtcgagg tctggttcga ccgcggctcc
    23941 acctggggcc tgccgtacga caccggctac gcctggctgc gcattgattc caacctcgtg
    24001 tacatcgaca ccgcgctcgt gcgcaatccc tacgtgacgg tggcgggggc ggcggccctc
    24061 gactacctcg cccagcccca gcagctccgc atcggcgcgc tccacgtcaa tctgctcgac
    24121 cgcagcttct ccaaccccga ggaaatgctc atcgaggtcg acaccgccgg tttcatgttc
    24181 cggcgggccg agatcggcaa cgccgacgcc cgcctgtcgc tggccggccg catcaactac
    24241 gacgagtcga tggacctccg cctcaccctc gagcgcctcc ccatcgcgcc ctggctcagc
    24301 ctcgcggact cgaccattcc gatggacggg tacctgtcga cgacggccgc ggtggcgggc
    24361 gatttccggt ccccggtcat ccggcttgag ggcgccgtgg attcgctgac ctacgcgggg
    24421 ctcgaactgg gcgacgcggt ggccgccctg agttacgccg acgcccgcct ggacgtcgat
    24481 tccgcctgca tctattcgga ttccggggcg taccgggccc ggggcgtgct ctatgccgac
    24541 ctcgctttca ccgccgattc gctcgagcgc tttccccggc ggcccatggc catccgcatc
    24601 gacgcccgcg accgcgactt cgacctcgtc agcctcctcc tgccctcggt cgaggacctg
    24661 cagggggact tcttcgccga cttcgagctc tccgggcatc cgcgcgatcc ccacgtccag
    24721 ggggaagcgt acatcaagaa cgcccgcctg aagtatttcg acctcgagaa ctggatcttc
    24781 gccgactcgg caggcgtgtc catgcacgac gacagcatcc tcatccaccg catcgaggcc
    24841 tatgcgaccg accgcaacga tccctccggc gtcggccgcc gccactatgc ctacatcgac
    24901 ggctccctcc agttgctggc gctcgacagc ctcgcctacg acgtcgaggt gcgcctggac
    24961 aacgaatttc ccttcaccta tgagcttgag gacattcggg ggaaggtcga ggggcggctg
    25021 ttcgtcgagg gccccactcc gccgacggtt tccggcgaca tccagctcat ctccgcccag
    25081 taccgcgtgc cgttcgccga ggagggagag ggctcgccca ttctccgggc gctcacctcc
    25141 cagaacacct gggacctcaa cgtcaacgtc gaaatcctct cgaacttctg gatcaagaat
    25201 gaagatatcg acgccgagtt cgccggcgag atcaacctcg tccgcgagcg gggcgtctac
    25261 cgcttcctcg gcgaaatgga ggtcctccgc gggcgcggtt tcctcttcga taaaactttc
    25321 cggctcgatc cgggcagcaa ggtgaccttc cagggcagcg acacgctgaa accgcagctt
    25381 gacatcaccg gctccacccg catgaccgcc gtgcgcgcca ccaccggcga cgaggggccg
    25441 gtgaccgagg ccatcgatct ctgcgtccac gtcggcggca cgctcgaaac cccggaaatc
    25501 aacccctgcg gcggcgagga catcgcccag tccgatctcc tcccgctcat cgtcaccaac
    25561 tactacgggg gcgacggtct ggcggtcggc ggccggctcg aggagcgcgt cctcggcctc
    25621 ggctatgccc agttctccca gatcggcggc cgacagctca accggatcgg cgtcgagacc
    25681 ttcgaaatcg acccggtcta cagcgaccag ctcggcccgt ggaacgcccg ggtgacgctc
    25741 ggcaaatacg tgggagaagg tctgtacgtg tacggccggt cgaccatctc cggccagacc
    25801 cgccaggagt acggctttga gtatcgctgg ttcaaggggg tgctgctgga gggccgccgg
    25861 gacgaggacg agctctacca ccttaacctg aggctgcact gggaatggtg agaatcgccc
    25921 gccgggtttt gcccgtggtg ctcgcgctgg ccgccgtcgg cgcctttggt ccggctgccg
    25981 ctgtcgaccg cgacatgctc cgcctcatca aggacaaccc gcccatccgc gacatcgtga
    26041 tcgacggcaa cgcgcactac tccgatggcc gcatccgcga gcagatgtac gcccggcccc
    26101 gcaccttctg ggcggccctc aagggcgacc gccgcagccg tctccagcgc gaaacgctcg
    26161 accgcgacac cctcgaaatc aaatacctct acctccgttc ggggttcctc ggcgtgcgcg
    26221 tcagcgaatc gttcgagccg atggagaccg gcgccggcgt ccgcgtgcgc acggtcatcg
    26281 acgagggccg ccgcagcgtc tacggcgacg tccgcattca gggggagtac ccgccgcgct
    26341 tggaatcccg cttccagcgg gccgtccgcg gcctcaagca aggcgctccg gtcaactact
    26401 tcgacctggt cgagaccggc ttcgagatga aggcggtcct cgccaacaac gggtatccct
    26461 acgccgccgt caccccggag gtcgataccg gcggaccgga tggcgcgacg gcgatcactt
    26521 atgcgatcac cgccgactcc ctcgtgcgat tcggcggcgt ctccgtgcgc ggcgtcaaac
    26581 ggtacccgga gtatacagcc gtccgcgaac tcaagttcaa gccgggcgac gtctaccggc
    26641 gccaggacct catcgattcc gagcggcggc tgttcgaatc cggctacttc tcgaccctgc
    26701 aactgcagca ggaggcgcag cagtccgacc gctaccgccc cgattttgtc cttcaggtgc
    26761 gcgagcgcaa accgtactac ctgtcgttca cgaccggggc cgggcagtcc gaggccgccg
    26821 acctgacctg gagcgtgacc tccggcgccg gcaagcgcaa cctcttcggc tcgcgccgcc
    26881 tcgaattcta caaccgcgtc gagttctcgc tcggggccga cgcccgcctc accgaccacc
    26941 tctaccgcct ccgctacacc gaaccctggc tgctgggatt ccgcatgccg ctggcggtga
    27001 ccctcgaata ccagccgcgc atccgccacg aggcgcagga tttcgacgtc gaggagtgga
    27061 acgcatcgct ctcgaccacc cgccgggtcg gccgcctcac cgatgtcacc gccggcatcg
    27121 aataccagtc ggtcgacatc agcggcgtcc cggctgaact cgaagaggcc gtgcgcgccg
    27181 aggtcgaggg gctgtccatc cgtcgccgcc tctatctgaa tctgcgccgg gacagccgcc
    27241 tcgatgtctt cgtgccccgc cagggctcgg tgcgcgacct ccgcaccgaa tactacggcg
    27301 gctttctggg cggcgacgag cacttctacc gcctcgaggg ctcgtggtcg ttctaccgcc
    27361 cggtctggcc gggctggatc tcggcgaccc ggttccgctc cggctacgcg cacgccttcg
    27421 gcgattccga actggtcccc agcaaagacc gcttcttcct ggggggcgcc aacacgatcc
    27481 gcggattcgc cgaaaacacg ctcgccccga ctctggccga cagcctgccg ggcgccaatt
    27541 ttaccgtcgt gtgcaaccag gaattccgct ggaaaaccct ccaggtcttc gccgtcgtgc
    27601 ccctgctgaa caagttgctg gagaacatgc ccctctggca gacggtcttc atcgacatcg
    27661 gcaacgggtt cacccgggtc agcgaattca gcttcagccg gctggcctat tcctacggca
    27721 ccggcgtcca gatcatctcg cccgccgggc cgatccggtt ggactacgcc cgtcggatcg
    27781 agaccgatga tatccccttt gatgatcgct ggcatttcac tatattgttt gcgttctgac
    27841 cgccctcccg gttgcggcgg gaaccggtca cggcgtgagc cggtttttcc ctagcaaccg
    27901 gcgcggtccg tcccggccgt cggtggttcg ctgaagagaa agggtgcaca atagagacta
    27961 tggatcaaat ttacctcgat cacaacgcga ccacgccggt ggacccggaa gtggttgacg
    28021 ccatgctgcc gtacctgtcg cacacgttcg gcaacgcgag cagcctgcac tacttcggcc
    28081 gcgaagccaa agcggcgctc gagcgggccc gtgaggaggt cgccgccttc atcagttgcg
    28141 accccggcga gttgtacttt acctcgggcg gcactgagtc cgacaacatg gccgtactcg
    28201 gcgcagcctt ccagcggcgc gaccgccgcc ggcacgcggt gtacggcgcg accgagcacc
    28261 acgccgtcat ggaggcctgc gagtatctgg tccaccgtca cgggttcacg gccgacgccg
    28321 ccccggtcga cagtgagggg ttcgtctcgc cccggcggct ggcccgcctc gtgaccgatc
    28381 agacggcggt tgtgtcgatc atgcacgcca acaacgagac cgggaccatc caggacctca
    28441 aaccgctcgt gcaggccgcc cacgaaaagg gggcgtggtt tcacaccgac gccgtgcagt
    28501 cgtgcgggaa gattgaaatc aacgtgcgcg atctcgggct cgaactcctg tcgctgacgg
    28561 gtcacaagat atacggcccc aaggggacgg gcgcgctgtt catccgcgac ggcatcaaac
    28621 tcacgccgct catgtacggc ggctcgcacg agaagaagcg ccgccccggg accgaaaatg
    28681 tcgccggcgc cgttgggctg gccaaagcgc tcgagatcgc ccgccgccgc atggaagagg
    28741 actggatccg cctgagcgac ctggccgact tcttcatcga ccgggtgctt accacgatcc
    28801 cgcacaccta cctcaatggc acccgcatgg gacgcattcc ccaggtctgc aacatctctt
    28861 tcaccggcgt cgaaggggag tcgatcctgc tggccctcga cctggagggc gtcgcctgct
    28921 cctccggttc ggcctgcacc tccggcgcca ccgaaccgtc ccacgtgctg gtcgccatgg
    28981 gaaaggaccg cgtggtcgcg cagggagcga ttcgcttctc cctcggccgc tccaccaacc
    29041 gggagcagct cgactacgtg ctggccaagc tgccgccgat catcgagcgt ctgcggtcgc
    29101 tttcgccgct gtaccaggaa cggatgaaat cgtaggcccg gaccgccggg tccccggccc
    29161 gcaggaagac gattgccatg cccccccgcg ttctggtcgc catgtccggc ggcgtcgact
    29221 cctccgtcgc cgcccttctc ctcaaagaag aggggtacga agttgtcggc gcccacatga
    29281 aactgtggga ctacgtcgac gtgggcggcg accggcaccg cgacggccgc tgctgctcgc
    29341 tcgacgccgt caccgattgc cgcctcgtgt gcgaccgcat cggcgccccg ttttacgtcc
    29401 tcaacatgtc cgcgccgttc cgcgccgccg tcatcgaaaa cttcgtctcc gagtaccgcg
    29461 ccggccgaac cccgaatccg tgcgtcctct gcaacaccga catcaagtgg agcggtttcc
    29521 tccacaaagc ccgcgagatc ggctgcgatt acatcgccac cggccactac gccgtcatcg
    29581 aacagggcga gaacggccgc tggcgcatcc gcaagggggt cgacgccacc cgtgaccagt
    29641 cctacgtgct ctggggcgtg tcccaggatg cgctcgccat gaccctcatg ccgctcggcc
    29701 gccggtacaa gaaggacgta cgcgcattcg ccgcacgcgt cggtctccgc accgctgcga
    29761 aacccgaatc gcgcgaaatc tgcttcgtcg ccgacaatga ctaccgccgc ttcctccgcg
    29821 agtgggaaca gaaacatggg ctcaagcgcc ggccggggga gatcgtccac gaggacggcc
    29881 gggtgctggg ccgccacgcc ggtacggtcg atttcacggt cggccagcgg cgggggatag
    29941 gcctggagca tcccctcccc gaacccgtct acgtgcagcg catcgacgcc atctccggcc
    30001 gcgtgacggt cgggtccaac agctcgctct tccgcgatga actcgtcgcc gagcgcgtca
    30061 actgggtggc ccatcctccg ccggcccggg accaaacctc aacgtatgct gcgggtgaga
    30121 accctggcgg tcctgccccg gccgccgccg agtcgcccgc ggccggcgct gcgacagcga
    30181 ccggggtgtc tttcgacgcc ctcgtcaaaa tccgctacct tcatacgccc ggccacgccc
    30241 ggataacgtg tctggctgac ggccgtgtgc acgtgaaatt caccgaccgg cagcgcgcca
    30301 tcacccccgg gcagtcggtg gtcctttacg acggcgacat cgtgctcggc ggcggcatca
    30361 tcgccggccc gtgacgccgg cgccccgccc cccttgactt cccgccgccc gcgcccttac
    30421 ttacgcagag aacgctgttc gtacccgtaa tcggtgccaa ggaggagtct catgccgcat
    30481 atccaatggc tcggccattc ctgtgtgacg atcacggaag gtccgcataa actcatcatc
    30541 gacccgttcc tgaaagacaa ccccaaaggg gccgccaagc cggagacgat cgacatcggg
    30601 tggatcctgc tcactcacgg ccacggcgac catgtcggcg acgcggtcgc gatcgggaag
    30661 cggaccggcg ccaccgtcat cgccagcttc gagctggcca acctctgcgc gaaagaaggg
    30721 ctgaaggtgc acccgatgca catcggcggc tcccacgagt tccccttcgg ccgcgtgaaa
    30781 ctgacgattg cccatcacgg cggcgggtac ggtccggacg cctcccggta caccgggccg
    30841 ccggtcggtt tcctcgtcac catcgggggg aagacgatct atcacccggg cgacaccggc
    30901 ctgttcctcg acatgagact gatcggcgag atgaaccgga tcgacctcgc tttcctgccg
    30961 atcggcgaca acttcacgat ggggattgcc gatgcggtca aagcggtcga gttccttcag
    31021 cccaaagtcg tcatcccgat tcactacggc acgtgggatc tgatcgccgc cgatcccgcg
    31081 gaattcgcgg gcaaggtccg cggcgcccgg gtcgagctgc tttcgcccgg cggccgctac
    31141 acgctctgac cgatttcgcc acggcgccgg gcgcttgccc ggcgcccccc gccaattgcc
    31201 ccccgcgaac attcttcccc cgccgcgtgc tataaagaac ggcgcggtcg cctcggcgcg
    31261 cgctttttgt tgcttttcgc ggtcggatgg cgagattatt ccgtgccctg gcaagggcca
    31321 acaaaagaag gtcgaaccat gaagaagctg ttgaagatag tcgcgtggat cgtcggcgcc
    31381 atcgccgcgc tcctcatcgt cgccatcgtc ggcgtgatgg tgttcttccc caaagagaaa
    31441 gccaaagccc tcattgtcga gcgcggttcg caggcgctcg gccggcccat ctccgtggcc
    31501 gcggtcgatg tatcgttctg gggcggcctc ggcgtcgttc tccggggggt ggcggtcgcc
    31561 aacccgcccg actggccgac cgccgatacg atgttgacgg ccgagcagat cgacgtgaaa
    31621 ctcaaactgc tcccgcttct ctccggcgag taccgcctgg ccagactggt catcgaccgg
    31681 ccggtgatcg tgctgcacaa gaccggcagc ggcgccgcca actacgcctt cccggtcacc
    31741 gacacggcac gggcggtgca ggcgctgctg gaggaaaccc gcgcggccgc cgtcgccgtc
    31801 agttttgacc ggctcgccgt caaccgcggg cggctcacct acatcgacga cagcacgggc
    31861 gtgagcgtcg ccctgaacga cttcaccctg tccacagcac tggccaaccc ccgggccaac
    31921 gtcttccgct ccgaggggaa cctggaaatt ccgcaactgg tcgccgtcct cgaaggcacc
    31981 gtcccgccgt tcgcgctcgg tttgcgctac cgggcggagt atgatctcgg ggcgaagaaa
    32041 cttctcctcg acgattcccg cgtccgcatc aatcaactga atttcgcgct ctccggcgaa
    32101 ctcagccacg ggggagcggc ccctgtcgcg cgcgggcaca tcgcctccga ccaaatcgcc
    32161 gtgcgcgatc tgttctcgct tttgccggcc ccgcagttgg ccatgctcca ggattacacc
    32221 atcgaagggg acttcgcgct cgaggcggat gtccgctacg acgtaggggc cccggacacg
    32281 ctcgattact ccggttccgc ccgcatcagc gacatgctcc tcgaataccg gcaggtgccc
    32341 ggccaactgc gtgtcccgtc cgccaccgtc gacttcaagc gcgacaacgc ccgcctcaac
    32401 atcagcgacg catcgttcgc cggaaagccg ctcaaggggt atgtctacgt cgaaaacttc
    32461 gccgcccccg tcgtgaacgc cgaactgacc ggggaaatca acctcggcct gctccggccg
    32521 tttctcccgg ctgcgaatcg ccccgagctg gtcggcgacg cgtcgttttc ggtcaaggcg
    32581 tccgggccgg tccgtcaggt ggaggagctg gaggtatcgg gaaatgtccg ggtggccaac
    32641 ggtcgctacg ccgccgccat gctccccgag ccggttgaat cgttttcgct cgacgtgtac
    32701 tttgacaaaa gcgtggtccg cgtcaacgcc ctcgatgcgc gcatgccctc gggcaatctc
    32761 tcgttcaaag ggcgggtcaa cgatcttctc ccttacctca tggccgattc ccttcacccg
    32821 gtgaaaaccg ggccggcggt cgacggcacg ctcgaggcgc aggtgaatct cgccatgctt
    32881 aaccccttcc tgccgaaaca gggaaaaccc gagattgcgg gcaagctcca gaccagcctc
    32941 cagttggccg gtgacatcct cgaaccctcc ggcctgcgcg tgcgggggac gctggccgtg
    33001 ggcgaggggg cctaccgcga taccgccctg gccgaaccga tcgaacactt cgacacgagg
    33061 atgacgatca ttcccgatac gatcaggatc gacgaactca acatcaggtt caccacttcc
    33121 gacctgagtc tcaccggatc gctgatcgat cccttcccgt atctgctgcc gctgaagtcg
    33181 gtggatcgaa cgcgggcggc caaacccttc ctccagttcg ccatgacctc gcaccgcttc
    33241 aatgtcgaca gcctctttcc cgacaccctc ccctccggcg acgccgccga cggcggtccg
    33301 gccgccggcg ataccctcgt ccccttcatt ctccccgaca tcgacggccg gggggtcgcg
    33361 cgcttcgata ccgtcatcta ctggaacgtg ccgttctccg atctcaccgg cgacgtcacg
    33421 atcaaagatc gggtggtccg cgtcaccaat gtcgtcggcc gcgtctacac cggcacggtg
    33481 acgggggaga cctccatcga cctcaacaac tggctcgccc cggcctacga cggccggttc
    33541 aacgccaccg acatcgaggc taatgatttc gcccgccgct tcaccaggtt cggcgatttc
    33601 gtgtacggca agttcaacct cgacggcagc tttcaggcct ccgggtggga tgccgacgcc
    33661 atcatgtact cgctgaacat gaacagcgcg gcgcgcatgc gcgaggggcg gctggaaacc
    33721 tcgggcgccg ttctcaccgc gctgcagacc gtcgggagtc tcgtcggcga acagcttggc
    33781 cgcaccttca acctccgcaa tgcccggacc gacatcgccg tcgacagcgg ccgcgtccgc
    33841 ttcgataagc tctccgccgc cctcggcgac atgggcgatc tcgaactgaa cggattctat
    33901 tcgttcgccg gcgaggtcaa ctacaccggg acgctgctgc tcagtcccga actcacgcag
    33961 aaattcctct cccaaggcgt gctcggcgag gtctcgagtt tcctgggcgg cagttccgct
    34021 gtgaaacagg tgaagctgcc ggtcaccatc ggcggttctc tggacgaccc gaaggttgat
    34081 attaacttga aatcgatcac cgaacaggcg ggcaagagcg cggtcgaggg cgccgccgac
    34141 aagctcaagg gcctgatcaa gaaaccgtag gccgcggccg gcccgcgccc gccggaccgt
    34201 cgcgtcgcgg ccgtccgcaa ccgatgcccc tctgacgggg cagaaccctc gccgatctgc
    34261 ggggcaattc tgccccgcaa agttgtcccc cgccggtgaa taatcccctg catgtgtccg
    34321 atataaccga agtacgggac gacggcgggc ggtccgccgg ggggagatgc cttcggctgc
    34381 cgccggcaag cggcgggcga tgttggaaga ggtttgcatg ccgacggttc gacagcagaa
    34441 accgggcgcc gcggtggaga tgaacgcccg cgcgatcgcc cgctgcggcg tgtgcaaggt
    34501 cgatctcaag gggcgctttg tctttgtcga tgagcaggct cagcagctct tcggctgccc
    34561 gggtgaagag ctgttcggaa aagccctcat cgactttgtc gacgaagcct cgcgggccgt
    34621 ggtcaacgag attctctccc gtcgcaacca ctacgaaatc acgttcgacc acattgggtt
    34681 gacgctcgtg gttcccggcg ccgccgccat ccgggccggg gcgatcgtca cgctcaattt
    34741 caacgccggc aaccccgtca atttccagtt catctttctg gccgataacg ccgttgccgt
    34801 cgacctcgtg cgcgacccgc acgcggccgc ctaccgacgg ctggtggcct atctcgacgg
    34861 ccgcgatctc accgccgact ggcgcggcta tctcggcgaa ctccagtcgt tctccggggc
    34921 cgtgcaggtg tgcgcctact tggtctcgcc cgaccggctc gagccgcgca gcagcgcgct
    34981 ggacgcgggc gacggcgtca cctcgcttcc ctcgatcgac ccgaccggcc ctctccaccg
    35041 ccaggtcgcc gacacggggg agacctacga tttcaccgac cagcggtcga tccagcgcgc
    35101 cgttgaactt ggcgcacccg ctccgaacga ggtgatcgcc cgcctggctc tcgacgaggg
    35161 tcaggcggct ctcatccggg tgatctttcc ttccgacatc gccgacaacg atgcccgcgt
    35221 acaggccgac cgcacccgct tcgccctcga gctcacccag cggctgctgg ccccgccgcc
    35281 cccctctgcc gatgaccgca cggccgacaa catccagttt gcgatcgggc tgctcgacgg
    35341 actcgggatc ggcgccatga tggcggactc gcgcggcaag accgccgtct acaatccggt
    35401 gctcgcccgg cttttccagg tggaggatgt ctccgtcgat ctggtggaac tggccgagat
    35461 tctctccggc ggcgccgccg aggagcggca gcgcctcgat gatttcctgc gcggcgcggc
    35521 gcgcccgagc accggcgcgc tggagctggc gcgcgtcgaa ctccccgccg ggcgccccgg
    35581 cctgctggcc gttctcccgc tggccgagaa tcccaacgac cgcaccgcct ttgtcgctat
    35641 tgtccccggc gctctggccg ccgacgccgc cgacgtgctc gcgttcgggc ggaccctcct
    35701 gagccgtctc ggagccgaca ccgccgaagc cgtggcgctg agcgcccggc tgggtcagag
    35761 gagcgcggct ctggatgcgg agggcaaaga ccagctccac cggctcaccg agtcgcttca
    35821 ggatctcggc cgcctgacgc aggatctccg gtcggcgctg gacatctcca gccgggttca
    35881 ggcgcccgaa ctgaccgacc tcaacatcgt cctccaccgc gtcgttaacc acgtcaaccg
    35941 cggccggggc ggccagccgc tgcgcgtcca cactgacctg atgcccaagg tctcaacgtc
    36001 ccggcgcgcc gtgacgctgg cgctggagaa cctgctccag gctgtcgcca ccggtgccga
    36061 ccgggcgacc gccaccgtgc ggatcgccgc cgaacttagc ggaccctcgg cctgccgcct
    36121 gacaatcacc gtcgactccc ccctcgatcg aagccacaac gacttcgcgt tcggtttcag
    36181 cgcggcccgg ctgctcatcc gcagcatcgg gggcacgctg cgcgaggagc cccgcaccgc
    36241 cggcgtcggc ttcgtgatcg ccttcccggt gcagccctga acgatcgttc gcggcgccgc
    36301 caaccctgcg ccgttggccg ccttgctcct tcgtggacaa gctcttagca gcagccggac
    36361 gagaaaagtg acacgccgct cccggttttg cggttgacaa aagattccca ccccctattt
    36421 tggaccctgt ctttcggcag gaattatgac aatggattca atgaccggtt tcggcaaggc
    36481 cgaattcaag tctgcactgg gtacgttcac cgtggaggtt tccagcgtga acaaccgttt
    36541 cctggaggta tccgcccgat tgccgaggca gttttcggcg atggagccaa aagtgcgcga
    36601 gttggtcggc cgccatgtga atcgcggcaa agtctacatc tatgtcggat tctccgaagg
    36661 ggaggagaat accgatcgat tctctattaa tgtgaaggcc gccgcggcct atgctcgcca
    36721 actggccgcc ctgcgcgacc aactccatat cgggggtgag gttacgatcg gcgacctgct
    36781 cgtgttcccc gaggttgtca aaagcgacga aaccgctttc gaggacgagg aaatctggga
    36841 gcagctcgag gccccgctgg ctgaggccat gaccgctctc gtgcgcatgc ggcgccgcga
    36901 gggagaagct atggcgcgcg atatgaagca gcgtctcgac cagatcaagg agatcacccg
    36961 ggcggtcgag aaggaggctg cccagagtgt cgaccgctac cgcgaccgcc tcctcgtcag
    37021 aatctcggaa ctcgctgaaa ctattcagct tgaccccaac cgcctggaac aggaagtggc
    37081 cctccaggcc gagcgctccg acatcaccga ggagtgcacc cgtatgttca gccacatcga
    37141 ccagtatgac gccgacctgc ggcgcgatga tccggtcggc aaacggctca acttcatcct
    37201 ccaggagatg aatcgcgaag ccaacaccat tgcctccaag tgtaccgagg cctcgattac
    37261 ccgcctggtg atctccatga aagaagaaat cgagaaactg cgcgaactcg tgcaaaatgt
    37321 ggaataacca gggcgatggc atcctcatga ccccgaccgg aaaaggaaaa gtcgtcatca
    37381 tttcatcccc ctcgggcggc ggcaagtcct cgatttgccg ccagctcctg gagcgaaacc
    37441 ccgcctggcg gttctcggtc tcctacacca cgcgcagccg ccggccggat gaagtccacg
    37501 gccgcgagta tttcttcgtc tcggccgacg aatttcgccg tctggccaag caggattttt
    37561 tcgcggaaca ctgccgtgtt catttgtatg aatacgggac gccgaagaaa ccgctggagg
    37621 acgtgcgggc cgagggcggc gtcgtgctcc tcgatatcga tgtccagggc gcggagaaga
    37681 tcaaggcggt gtttgccgac gcgatcacga tcttcatcct ccccccgtcg atcgcggcgc
    37741 tgcgcagccg cctgagcacg cgcggaaccg agacgcagga acagcttcag gtgcggttcg
    37801 aaaacgccac ccgggagatg cgcctgtggc ggcgtttcga gtacgccgtg atcaacgata
    37861 cccttgcgac cgccgtgcgc gaggtcgagt cggccatcat cgcccaccac tgccgtaccg
    37921 accgcctggc gccggaacaa atagaaaaca tcattggtta atacgaccgg tgcggaaccg
    37981 gtaaaggaga caggcgtgaa gaaagtcccg atcgagcaga tcgaaaaaac cggcctcaat
    38041 cgttatgaag cggtcattgt cgcctccaag tatgcgcgca agctcaacac caagcgcctc
    38101 accctgttgg agcagatgga agacaacccg gagatcgaca tcgactcccg gaagattacc
    38161 atggtggcgc tgaatgactt aatcgagggg aaggtcaaat tcgaacgccc ccgctgagtt
    38221 cacccgttaa tttccggcat cccgttccga caacattgaa ggtatggcga aaaacctggt
    38281 catagtcgag tctccggcca agtcgaaaac cctcgcacgc ttcctcggca aggatttcga
    38341 catcatggcc acgatcggcc acgttatgga cctccccaag tccaagctgg gcgtcgacgt
    38401 caagaacaac ttccagccgg actacacggt gatcgagggg aagcagaagg tcatcaccga
    38461 gctcaagaag gccgccaaga aggccgagac cgtctatctc gctcccgacc ccgaccgcga
    38521 gggcgaggcg attgcctggc atgtcgccaa ctcgatcggc aacagccata aggccaagtt
    38581 tcgccgcgtc actttcaacg aaatcaccaa gggcgcagtc ctcgaggcga tcgccaatcc
    38641 gcgcgagatc gacatgaatc tcgtcaacgc ccagcaggcc cgccgcgtcc tcgaccgcat
    38701 cgtcggatac accgtctccc cgttcctctg gaagacggtc gcccgcaacc tctcggccgg
    38761 gcgcgtgcag tcggtcgccc tccgccttgt gtgcgagcgc gaagccgaga tcaacgcttt
    38821 cgtctcccag gaatactggc agctcacagc cgaactcgag accgccggga aggagcgtct
    38881 cgaggcccgc ctctacaaga tcggcgagcg gagcgtggtc aaacccggcg attccaacga
    38941 caacggcaac aagatgtcga tcggctccga gaacgaggtg cgcggctaca tggcggagct
    39001 ggagaaagaa tcctgggtcg tcgccgagat caagaacacc gagcgcatcc gccgcccctc
    39061 cgcgcccttc atcacctcga cccttcagca ggaggcggcc aaggcgttcg ggttttcgcc
    39121 caaagtcacg atgcgcctgg cccaggagct ctacgagggg gtcgatatcg ggcaggaggg
    39181 gccgaccggt ctgatcacct acatgcgcac cgactcgacg cgcatcgcca acgaggccct
    39241 caaggccgcc cgcgaactga tcgcccgcga ctacggcaag gcctacctgc cgcccaaacc
    39301 gcaggtgtac tcgaaaaagc agaacgccca ggacgcccac gaggcgatcc ggcccaccta
    39361 tctcgcgctc ccgcccgaca aggtcaagcg cttcctcaac gcccgccagt tcaagctcta
    39421 cagcctgatc tggaaccgct tcgtcgcctc gcagatgaac ccggcccgct acgacgtcga
    39481 gaccatcgac atcgccgccg gccgcttcct cttccgggcc gccgcgcagc ggctcacgtt
    39541 cgacggctac ctgaagatct accatgagga agttgaggcg gatgacaacg gcaacggcaa
    39601 cggcgccgcc gcggctctgc cccggctggc cgaaaaggat cccctccggc tcctcgagct
    39661 ccggccgaac cagtcgttca cgaagccgcc ggcccgctac tccgaggccg ccctggtgaa
    39721 gcggcttgag gccgacggca tcggccgccc ctccacctac gcctccatca tttccaccct
    39781 ccgcgaccgg aaatatgtca agctcgaaca acgcaagctt gcgccgaccg aactcggcct
    39841 gacggtgaac aagatcctcg tggaaaacct tcccaaactc ttcaacgtca aattcaccgc
    39901 cgagatggag aaagagctcg acctcatcga agacggcacc gatgactggg tgaaggttct
    39961 caatgagttc tacgagcctt tcaagaaatc catcgaggtt ctcaagggac ggcagaagga
    40021 aatcaaggag gcgatgaccg aggaaaccga catcaagtgc gagaagtgcg gttcgccgat
    40081 ggtcatcaag tgggggcgca acggccggtt cctcgcctgc tccgcctacc ccgagtgcaa
    40141 gtccacccgc ccgcttccgg aagaagaggc tgcccgcaag accgatgaag tgtgcgagaa
    40201 gtgcggagcg ccgatggtca tcaagaccgg ccggttcggc cgcttcctcg cctgctcggc
    40261 ctacccgaac tgcaagaata ccaaggcgat cacgctcggt atcgcctgcc cgcaggacgg
    40321 ctgcaaagga cagcttgttg aaaaacgcac ccgcggcggc aaaatcttct acggctgttc
    40381 cgcctacccg tcgtgcaagt tcgccacctg ggacaagccg gtgccgcacc cctgcccgca
    40441 ctgcgcatac cccttccttg tggccaagga atccaaggcg aagggcgaat acctccagtg
    40501 cccccggtgc aaggcggtga tcgccccgga ctcggtcgag gcccacaccc cggcacacta
    40561 attcggaatc cccgatcggc ttcggtcgcc ccgggccggg cccgcgctcg aatccggccg
    40621 cccggttgcc atcaattgta ttgacctccc tccgaccgcc ccgtatttat actcccgcgt
    40681 atgctccagc aggatctgac cgcatatttg ggcgctctcg ccgaggagcg ccgctgttcc
    40741 cctcggactg tcgaggccta ccgccgcgac cttgaaccgt ggctggcttt tctggccgag
    40801 cagcacgccg ccctgccggg aggccggccg aacgacccgc tcctgctgcg cgtctacctg
    40861 cggcggcggg ccgaagccgg cgtgtcgaac cgctcgctcg cccgttttct gtcggccctc
    40921 tccggtttcc agaaattcct ggctgcccgg ggaagagccg aggtctggct gttcaaactc
    40981 ccgcggatga agtacccggc caggatgccc cggttccttc cgcagaagga ggccgggcgg
    41041 ctgttcgagg aaccgccggc ccaacagccg gagcgcacct atttctaccg gcgcgacttt
    41101 ctcatgatcg ccctcctgta cgccaccggc attcgccgcg aggagctggc cggcattcgg
    41161 ccgggggatc tcgacctccg ccaggggctc atcacggtca ccggcaaggg gaacaaggtc
    41221 cgcgtcgtcc ccatcggcga cgcaacgctc aaggatgtgc gcgcctacct cgatgaccgc
    41281 gcccgatttc tcgccgaccg gggcaccgcc tgcgacgccc tgctgttgaa ccggtatggc
    41341 ggcccgctgt cggtgcggtc gatcgaccgc ctggtgaaac ggttcgggcg gaggtgcggg
    41401 ctggatttca ccccccacgc cctgcgccac tcgttcgcca cccacctgct ggaaaacggc
    41461 gccgacctga tggtgataaa ggaaatcctc ggccacgcgt ccctctcgac cacccagaag
    41521 tacacccacg tgaccgcgga agcgatgaag aaagtctacc gcaaggcgca cccgcgctcc
    41581 ggaagcaaag agtgaagggg agactatgcg cacccgttcg acgacgatta tcggaatcat
    41641 tcacaacggc cgcgcggcca tggcgggcga cggtcagatc acgttcgagg acacgattgt
    41701 caaggcccgc tcctcgaaaa tccgcaccat gcgcgagggc accatcctct gcggcttcgc
    41761 cggcacggcc gccgacgccc tcgcccttta tgaactgctg gagaagaaga ccgacgagta
    41821 cgccggcaac ctcgcccgcg ccgcggtcga actggccaaa gaatggcgga ccgacaaggc
    41881 gcttcagaga ctcgaggccg tcatcgccgt caccgacggc cgccacattt ttctcgtcag
    41941 cggcaacggc gacgtgatcg aacccgacga ccagatcgtg gcgatcggtt ccggcggttc
    42001 gtttgccctg gccgccgccc gcgcgctcgt ctgggccaac cccaaactga gcgccgacaa
    42061 gatcgcggtc aaagcgctcg agatcgcagc cggcatctgc atcttcacca acgcacacat
    42121 cactgtggaa acgctcggat gaaccagtcc taccccacgc cccgggaaat cgtcgaccac
    42181 ctcgacaagt acatcatcgg gcaaacccag gccaagaaat cggtggccat cgccctgcgc
    42241 aaccgctggc ggcggcagca ggtcccggcg ccgctccggg ccgagattct gcccaacaac
    42301 atcatcatga tcgggcccac cggggtcggc aagaccgaga tcgcccgccg cctggccgat
    42361 ctcgccggcg cgccgtttct caaagtcgaa gcctccaagt acaccgaggt cggctatgtc
    42421 ggccgcgacg tcgagtcgat gatccgcgac ctggtcgaca tcggcgtcaa catggtcaag
    42481 tcggagaaaa tggaggaact ccgtgcccgc gccgcccgcg gcgccagcga acgcctgctc
    42541 gatttgctcc tggcgcccgc cgaaccggag gctccggccg gggacggcgg ggaacggccc
    42601 gcctcggatt cggtccgcac cacccgcgcg aagctccgcg agaaactcat cggcggggag
    42661 ctcgacgaac gcgaggtcga agtcgaggcc ccccagtccc agttccccac catcgaaatc
    42721 ttctccccca tgggcatgga ggagctgggc gtcaacttcc aggagctctt ctccgggctg
    42781 atgccgaaaa agacgcgccg gcgcaaaatg tcggtgcgcg aggcccaccg cttcctgacc
    42841 gcccaggaac tcgaccgcct ggtcgacatg gacgaggtga tcgccgaagc cctcggccgc
    42901 gttcaggaag cggggatcat cttcatcgat gaaatcgaca aaatcgccgg cggcggcgac
    42961 cgcaccgccg gtcccgacgt ttcccgcgag ggcgtacagc gcgacatcct ccctatcgtc
    43021 gagggcagcg ccgtcatgac caagtacggc atggtccgca ccgaccacat tctcttcatc
    43081 gccgccggcg ccttccactc ggcccgaccc tccgacctta tccccgaact ccaggggcgc
    43141 ttccccatcc gggtcgaact cgactcgctg accgccgatg atttcgagcg catcctcacg
    43201 gaacccaaat cggcgctcgt gctccagtac caggcgctgc tgaaaaccga ggggatccgg
    43261 ctggagtttc agaaagaggc ggtgcggcgc atcgccgagt ttgccgagcg ggtcaacagc
    43321 gaagcggaaa acatcggcgc ccgccgcctg cagacggtca tgaccacgct gctcgaagaa
    43381 atcctcttcg aaccgccgga aaagaagaga gtcgtgatta ccgcgaggat ggtcgagaag
    43441 accctctcgg ccattatcga aaacgaagac ctgagccggt atatcctgta gcggaaagga
    43501 gcgccgggcg gcttcctaca cctggtagac cttgatgtcc ccgaggaagc tgttggcggc
    43561 gatgtacagc cggcgttcgg cggcggcgta gttggcggtc tgcgcgtcga gattgctgct
    43621 gaaacccgag acccggcgcc cgagcaaatc gatgtcgccg atgaaattcg agcagtgcgc
    43681 gaggaccgcc atgtcgggcg gcaacaggat ccgtatatcg ccgacaaacc cggagacgat
    43741 catccggcac aaccccccct ccagtctggc cccgtggaca cggatttcca gatcgccgat
    43801 tcccatcgaa acctcgacgt gctgcacggc gcggtcccgc agatcgacgg tcagatcgcc
    43861 gatcagcttg ctgtagcgga gcttcccgtc ccgcatgaca ccgtactcgg gggattggaa
    43921 ctgcggtccg ccggccgggg ccgccggatc ggccgatcct cctgcgggtg cgtcgccggc
    43981 cggggccgcc gtgctttgcg ccccctcatg cgcccagaaa gtctgcggag ccggtccggc
    44041 cggagagggc gagggcgggg gcgggggagg aggggccgac gccgcttcga aactcagcgc
    44101 cgcatcctgc tgcttgcgcc ggatgatcag ccagattccc agcgcaatga acccaagcgg
    44161 aacgaggtag cggagcacct ccccgaccga catcacgtcc agcgaattca acagaaagag
    44221 gaaccccaga aggatgagga taatgccaaa tgtcgcgcgc cgtccggtca tcgtcgtcaa
    44281 cgtcctttct catccccaca gttcattacg gaatatcggt cgcccggttc cggttggcaa
    44341 gggattcggc gggctttgtc gggcagcccg gggtgacgac ggctgtccgg ccccccccga
    44401 cttgacatcg ccccaaccat cctctttatt ccccaaaaca cgactgaaca cggaaaaatg
    44461 acactaccgc ataaggagac gccatggccc gctcgctcac gcagattacc gacctgacgg
    44521 ccgacgaggt ccgccggatt ttcgacctgt gcgcccggat gaaatcgggg cagatcgccc
    44581 ccaagccgct cgccggcaaa gcggtcgcct gtattttcac caaaccgtcg ctgcgcaccc
    44641 gcatcagttt cgaagtcggc atcaaccagc tgggcggcca cgccctctac attaccgaca
    44701 acgagatcaa gctgggtgaa cgcgagacga ttcaggacgc cgcccgggtg ctctcccgct
    44761 atatcggcat gatcatgatc cgcacgttca agcagtccga cgtcgagggc ctcgccaagt
    44821 acgcgtccgt gcccgtcgtc aacggcctga ccgacctcgt gcatccctgc cagattctcg
    44881 gcgactactt caccgtcatg gagagcttcc ccgggcggga gcgcttcaag atcgcctacg
    44941 tgggcgacgg caacaacatc gtcaacagct ggctcaacct ggcctcgctg attcccatgg
    45001 atttgcggat cggtacggcc gccgacaccc agcccgacgg ggctattctc cggcgcgccc
    45061 aggcaaaccc ggccagccgg gttctcctca ccgaggaccc taaagaagcg gtcgccgggg
    45121 ccgatgtagt ctataccgat gtttgggcct cgatggggca gaaacatctg gccgagcaga
    45181 aagcagagaa gctgaaaaaa taccaactca acagctccct gctggcgctg gccgatccga
    45241 aagctatcgt gttgcactgc ctccccgccg agcggggacg cgagatcacc gacgaagtga
    45301 tcgacggacc gcactcgcgg gtcttcgacg aagcggagaa caggctgcac attcagaagg
    45361 ccatcatgac cttcctgctc caatagcctc ttcggccggc cgcccgggag gagctgtctg
    45421 cagagaaagg agacgcagat gcgttctcac aggtctttgt tcctctcggg cgcggcgctg
    45481 gctctggccg cgctggcttt catctggggc tgcagcgacg acgacgacaa cccgaccaag
    45541 tgggggagcc tcgaggatcc ccagttccag gcggccaata cccagtttga ggcgctgctc
    45601 gacaccacca ttacccagtt caccgaggcc ctcggggcga ttcagaccct gcccgccaac
    45661 gacgacgatc cgattcacca tggtcccggc gatccgaacg gtatcaccga ctccctgatc
    45721 gtctcctaca gctccggctg gcacaccatc cacctggaat ggcacagcca gagcggctac
    45781 atcgccatcg tcaacgactc gatccagttc ctgcgcaacg gccagccgac ccaggagggc
    45841 tccggcctcg agcagctcct ctaccgccac cggtggtcgt ggaccgctcc cgacaccacc
    45901 gtcacctaca tcaacggcgc gggctacagc ctgttcgatt ttgacggact ccacacgcag
    45961 gaagcgacca tcgacggcgc caacggctgc agcgtcgaga ccaagttcgt ctttgccgac
    46021 tcggtcgtgc actatgactt cgccgcggaa gcggcgctcg acgacgtcgt cgtgaccaaa
    46081 accggcccca cctgggaccg cggctgcccg acctccggcc ggttcaccgc gaccatcatg
    46141 atgacctacc agcgcaacga cgaagcgccg gtgccgactc agtggaattt cagcgcgaca
    46201 ttccacaacg gcgccgtgca cgtcgtcgcc accgccaacg ggtacacctg gacgtacgac
    46261 ggcgtcgcct gctcgcagga aggctgacgc ccgctgcgta ctctcaattt ccgcgcccgg
    46321 ccgccgaccg cagccgggcg cgaacttttt ctgcctcggc ctgcacggtt tgatctcccc
    46381 gacgccattc catcgttgac tttgcccgtc gccgcctcct actatcggct atgagagaag
    46441 tccccaaata tcacggctgc ttcgtctgcg gcgacaagaa tgcctgcgga ctgcaggccc
    46501 gcttcttttt cgacggcgag aaggttctca ccgaggctgt ggccgacgaa cgattcgagg
    46561 gctattgcgg cattgggcac ggcggcatta cggcggcgct gctcgacgag gtgatggtca
    46621 aagcggtgtt ggcggcagat gtctgcgccg tcacggccga aatgaccgtc cgctaccacc
    46681 gccccgtcta caccggcgac aagctcttct gcaccggccg ggtcgttaaa cagaggggcc
    46741 gggcgtggtt tgccgaaggc gagattgtcg atgccgacgg ccgccccttc gcctccgcca
    46801 ccggcaaata catcgaggcc gaagaagatt tccgtcgcct gctcctgcgc tcgctcgagt
    46861 gacggtgcgt tcctcggcag cgagctgcac tcggcccggc aagccgctgc cgctgcgacc
    46921 cggcaggtta tcctaagttc cgccgccgcc gcgacctacg accccgccgg ggcccccgtg
    46981 gcgtcgggca cagggattgc tcaacggtcg tgcatggaag cgccggtgtg tcctctcacc
    47041 tgctatcttg cgctgcgccg ctgccccttg gccattccgg accctgcggc cgatgatcgg
    47101 atggtctcgg caattgtcag cttcgccatc cccgactacg ggattctcta ccggacgcgc
    47161 atgaccggcg atatcctcga tctggaattc cgcgcgctct ttgccgttct cgggttcatt
    47221 caaaatcgcc tcgccggaga gaagatcgag gcggtgcgga ttctgtcctc gactccggag
    47281 ttcatcttcg ccctgacccc cgagagcccc caccttcttt ctaaccgaaa gcgccgcaat
    47341 ctgctgcgga aattctgcgg tgccatcaaa gtcaccgccg cctatgtcag gcaagcggac
    47401 aatcgggcct tgctctcgcc ggccgacctg cccgccatgc cccggggcgc ggaaattgcg
    47461 ttctccgagg agcctgagcg aaggtccgaa agtggttccc tgcctccatt cggcgtcgat
    47521 atctaacagc gccgcgcttt ttcttccatt gacaatctgt tgttaccgta taccttagtt
    47581 gtgatacgtc gtgtccgcgg tgcggtactc gcaataaacg ccttcgcaat acgcacgtct
    47641 atgctatgat gcaaataccc agaggacctc ggtccggagg ctcgaaatag tatgaagaaa
    47701 ttgtttggtt cgcttgccct gttgctgatc gcggcgaccg gttcggcgac cgccgaagac
    47761 atcgggtgga tcaccgtgcg caatcagttc caggccgatc tggccgcgca ggtggtcggc
    47821 cccgcttaca cgcgcgatgg caaccggttt ttggccggcc tctcggcaac gaaggagcag
    47881 ctcctgcggc agtcgggatt ggcggtcgag attgtctaca ccgatgtcga tccggcgggc
    47941 gtgtaccgcg tcatgcccaa tggaccggcg ccgcgcgacc gcatggatat gcaggcgctc
    48001 ggccaggcag tcccgatcaa cgagacgatg tcgctgctcc agaccgaccc ggccgttgcc
    48061 cgcgaactct ccgagcggcg cggctgccac gcccaggcgc tgaccaccct gagcgtccgc
    48121 tggctctacc tgcccaccac cgtcgccacc ggtctgctgg atatcgacga tttcccgacc
    48181 gacaccctca tcaacctcgt cagccaggac tccatcttcg cctttgacga gcgcctcatg
    48241 aacttccgga cccgctacat cttcgccgac tcgatcatcg ccgccctcaa ctggatcgtc
    48301 cagaaattcc agagctgggg gtataccgtc acccttcagc cgttcagtta ctacgggacc
    48361 cactacaaca tcgtcgcgac caagcccggc tacgccgagc ccgacaagac catcgtggtc
    48421 ggcggccact acgattcgat cgtgtatggc gacggcacgg atccgtacgt gtatgcgccc
    48481 ggcgccgacg acaacgcctc cggcaccgtg gccgtcatgg aaatggcccg cgtgctcaag
    48541 gatgttcccc tgcgcaagac ggtcgccttc atccccttct ccgccgagga ggtcgggctg
    48601 gtcggctccg cctattcggc ggcccagttc gccgcccagg ggaccgacgt cgaggtgatg
    48661 ttcaactacg acatgatcgg gtacgatccg agcaacgagc gtctcatcgc cctgagcgcc
    48721 ggtccggcca acgggcacta ccgcgatctc cacatcgcca ccattgaacg cctcaccgac
    48781 ctggtcacga tgcccgagaa ctcgcccggc gggtccgatc accagtcgtt catcgatcag
    48841 ggattccccg tctccaacag catcgagggg acgttcaact accccggctg gcacaccaac
    48901 ctcgacctga ccagcgagat gaatttcccg tttttcacga aagtagtgaa agccggtctg
    48961 gccgctctcg ccatcgtcgc caactccgcc cggccgactg aaatcgagcg tctggtggat
    49021 atgggcgacg gccagtcgat cgaggttatc tggaagagct gcgatccctc ctaccgctac
    49081 gtcgtgcgct gggggacagc ctcgggcgtt tataccgatt ccgccgtcgt cccgccgggc
    49141 ggctgctcct atgtcgtcac cggcctcacc gagggccagc cgtatttctt cacggccgaa
    49201 ggcagcgtcg aggactccta tccgtcgatc tactccacgg aggacagtct gacgccgctg
    49261 gtggtgccgc gccggccgag cgccctggtg gccgacccgg cgctccaggc catcgccctc
    49321 tcctggcgtc ccaacaccga gggagacctc accggctata agatctatcg gaaaatcgag
    49381 gactacgact atcagttgct ggccgggggc gtcaccggtc agtcttatac cgacagcgcc
    49441 atcgcgccgc actccctctt cacttaccgg atctccgccg tcgacggtga tgcccatgag
    49501 tcggagcttt cgcccgaatc gggcaactat gccgccacgt tcgacggcgg cattctgatc
    49561 gtggacgaaa cctcccagca cccctcggtc ccgacccagg cgggccaggt ggccttctgg
    49621 gacacgatcc tggggcccac gccgtattcc cttgaggtga ccgatttcgc cggcgccgcg
    49681 cttcggcgcg ggatcgccgg gcagtactcc tcgatcttct ggttcgacga cgatttcacc
    49741 aaccacttcc tgaagacctc acaggactcg ctcacctggt atgccggcta caccggcaat
    49801 attctggtcg ccggtctgcg caccgtgcag tactggacca gctccgtcat ctaccccgac
    49861 gacttcgtct acaccgactt cggcctcgcc tcctggacgg agaactcggc cttcgatttc
    49921 gccggcgccc acgggcagaa cggctggccc agcctgacgg tccgcccgaa cgtcttcgac
    49981 aacctgccct atgtgccgag tctccagctc gctcccggcg ccaccctcct gtacacgtac
    50041 aattcggcca ccgacaatcc ggcccgcgag ggccaaccgg tcggcattct gaaaaccacg
    50101 ccccacggcg cgagaatcgt gctggcgttc ccggtcatgt tcctcaccga ctcctcggcc
    50161 acgcagctga tgaacttcgt caaagcccag ttcggcgaat ccggcagcgt ggaggagtat
    50221 ggggacgtcg atggttccgg gtcggtgaat gtcgccgatc tgacctactt cgtcgcgtac
    50281 atgtttcggg gcgggccgcc gccgccggtg ctgaacaacg ccgatgtcaa cggcgactgc
    50341 gaactcacgg ttgccgatct caccgcgctc atcggctatc tgttccgcgg gggaccggcc
    50401 ccggtggccg gttgcgtgga gtaggctgca acgacaagag gtctcgggcg aaaacatgga
    50461 ccggccggga gacagccccg gccggtcttc ttttgggcgc aagaccgccg gaatccgccc
    50521 tatgaccggc tgtgcacaat ctgtgtcggc cgcagccccg gccccgagtt ttggcgcccc
    50581 cgccgcggcg ccgggacaca attcaacctg ttggtaatta gacgcttgtc tcagcccggc
    50641 aacgacggca cagggcttgc tccactttcg cccatggagt ttcacgcact gcagcacctg
    50701 cgtatcatcg atcaggaccg tatcgccccg tttgaatggg atgatggcgg gcgtattgac
    50761 ctgtcccctg aggggccggg gatctttcgc gaccctctgc tggtcacccc gctggagggc
    50821 gacgactttc ttcttttgga tgattcccgg acctactact cgctggtcga tgccgacttc
    50881 gagcacctcc cggtccagat ctgcaccccg gtcgagttga tggtggacag cgatccgctc
    50941 agcctgaccc ggttcaccca cgatgatctc acccgcgtcc tggcccgttt cccggaccgg
    51001 attgagatcc tccccgccga gaaaaccgtt cccgactccc ccgaccatct cgatctcata
    51061 ttcggttttg tcggcgccgc gccgatccgc gttcggatcc gccactccag cctcaccggc
    51121 tgtccggtgc cgttcgatgc ggtctttcgg gccatcctcg aaagcggggc tttctacccg
    51181 gtcgtccggg acgggggaga gggggagtgg ggcgaccact ccggaccgca ctcggggacg
    51241 gtgagcctgc cgaccttcac cctcgaggac cttcgccggg ccgtgtccag cggacggctt
    51301 ttcccgcccg gtctcatccg ggttcaggcg gtgtgccgcg tgctcaacat cgacttcccc
    51361 gttcccgtgc tcgccgatcg gtctacgctt gagcagaagc aggcatttct ggccgagctc
    51421 atcgctttgc gggcgcggcg ccgccggatc gcgtgttttg acggccgggt gtacatgctc
    51481 aaccgctgag ttcccctccg gcgcccgtcc gggcggccgc gttctgctcc cctctgaaaa
    51541 tacttgttga agcccctgcg cgcgccccgc tacttttcat cccatggaga acttcgcgcc
    51601 ggccgccgcc ccaacgccga acgcccggct gcgttgccgc gccgggcgct gtgctcgatc
    51661 cggtgtcgcc ggatcaggcc ggtttgctcg ccgcgtgccg cgccagccgg gatttgtacc
    51721 ggtcggccgt gcgccggtgg agcaggcggc gggaggccgc ccggtcgatc atcgagacca
    51781 cggtgcggta tttgccggcg agatccgggg ccccgggttc cgtcgcccgc aggtcgcgga
    51841 tggcgccgcg caggtgggac cgccaggccc ggttccggtc ccgctgcttc tccgactgcc
    51901 tcatccgctt gaagttcgat ttgtgatgcg gcatactatc ctttcacatc ttcctttgct
    51961 ttcagaaata acctttaaat atacgcggca cgggcagctt gtcaactcct gaggcaaaga
    52021 aaagttccct cctgggatca gccacaacgg tgtccgcctg gactgccttc tcccgcgtcc
    52081 tcggcctcgt tcgcgaacag gtcatggcct tcatcttcgg cgccggcatg gccaccgatg
    52141 cctacgtcac cgccttccgc atccccaacc tcctgcgcga catgttcgcc gaaggggccc
    52201 tgtcatccgc cttcgtcccg accttcaagg agcggctggt cgccggcagc aaggacgagg
    52261 cttttcacct ggccaacgtg gtcatgaccg ccatcctcgt cgtcgtcggc ctcgtggtgc
    52321 tcctcggcat cgtcgccgcg ccgcttctca tctacgtttt cgcgcacggt ttcaccgccg
    52381 atgccgtcaa gttcgacctc acggtgagca tgacccggat catgatggcg tacctgctgc
    52441 tggtgtcgct ctcggccctc gtcatgggca tgctcaattc tttcggccgc ttcgggctcc
    52501 cggcgctctc gccggccatg ttcaacctcg gcaccatcgt cgtggtcctg gctttctaca
    52561 aggtgatgga cccgccgatt tacaccatgg cgatcggcgt gcttgtcggc ggtctcggcc
    52621 aggtcgccat tcaactgccc gccctcctgc gcatcggcta ccgcttccgc ttcaccatct
    52681 cgctgttcga tgccggtctg cggcgggtga tgcggctgtt gacgcccatg attatcgggc
    52741 tgtccgccgg ccgcatcaac attctcgtct ccacgctcat cgcgtcattc ctcgcggaag
    52801 gggcgatctc ctacctcaac tactcctacc gcctcatgca tttccccctg ggcgtgttcg
    52861 ccgtcgccat cggtaccgtc gccctgccgc aggtggccga actggtggcc cgccgccagc
    52921 acgaccgggt cggcgatacc ttcctgaacg ccgtcggcct gaactccttc gtggttatcc
    52981 cctccgccgt cttcctcgcc ctcatgggcc gtcccctcgt cgacctcgtc taccagtggg
    53041 gggccttctc cagcctcgac agcgaacaga cggcgctcgc cctgctgcac tactcctacg
    53101 gcctggtcgg cttcgccgtc gtccgcgtgg tcgtgccgtt ctactatgcc tatgaggact
    53161 cccggcttcc gatgaaaatc tcgatcctcg ccgtcgtcgt caacctcgtt ctctacgctc
    53221 cgctggtgtg gctgctcggg tttgccggac tggcggcggc cacctctctg gcggccctgc
    53281 tcaacgccgg cctgctgatc tggtttctgc cgcgcaaggg agtcgccgtt tcgttcggcc
    53341 gtctggcctt tcacacggcg cggatcaccg tgatcgcgct cgtggcgttc tatgttgcgc
    53401 gcgtcatccc ttatgacgcc gtcatcgccc tcgatgacgg tctcccgcaa cggaccctgg
    53461 cggtgattct tccggcgctg gtgggcgccg tgctctacgt tgctttttcc ttggccctgc
    53521 gtactcgcga gattaccttg ttgagaaaca ggttagtgcg aagacgtcgg gcggggaatt
    53581 gatggcgcgc gcgtatccgg ggatattcct tctcattgct cttgccggcg gcattctcgc
    53641 cgctgatttc ctccgcgcct ccgccccggc ctttttgctg gccgcgctgg gcgggttggt
    53701 ggcggctgcc gccgccctgg ttttccggcg accgtcggct gccgccgtgt ttttcggcct
    53761 cgtcttggcc ggcctctcgg ccgcacattt cgcccgcgac acctatgatc tcggttcccg
    53821 cagcctcggc cgctacgccg atagccgcac ccgctatcgg ctggttggcc gcgtcgatga
    53881 ctggccggaa ctccgccccg accgcatcga gctgaccgtc cgcctcgatt ccctcggacc
    53941 cgaccgcatg gccgcggtcg agggccgtct cctcctgaga atcgccgata ccggcgcggc
    54001 cgtgcagttc ggcgaccggg tcgagctctc cacccgcctg tatccggcgg ccgagagggg
    54061 agagggggcc gcctcccgcc gatcgctgct ggtaaagggc gtgggagcgc tggcttatgt
    54121 ccgtgcctct caggacgtgc gccgcctgcc gcgcccggcg gttgggctga tccccgtggt
    54181 cgacactgtg cgcgccgcca tcgggcagtc gctccgccgg acactgagcc cccgcgccgc
    54241 ggcgctggcc gggggttttc tcatcggcga cacccgcgac atcccccccg acctgtatcg
    54301 cgctttccgc gactcaggca ctttacacgt gctcgccgtg tccggttcga acgtggccgt
    54361 ggtcgtgggt ttcgtcctgg tgctcctgcg gcccttccga accggccggg tcgcgcgcac
    54421 cctcattctg cttgtggtgg ttgtgttctt cgcgctcctc tgccgcggcg aaccctcggt
    54481 tgtacgcgcc tccgtcatgg ccgctctcat tctgctggcc cgcctcgcgg ggcggcgcat
    54541 cgatctgaac aacctggtcg ccctggccgc cgccgccatc ctcctgtgcg cccccgccca
    54601 gttgttcagc gtcggttttc aattgtcgtt cgcgaccgcc tgggggctgg tcttcgtcac
    54661 gccccgggtc taccggcttc tcgaaggccg ggcgcttggc ccgccggccc ggtggttgtc
    54721 gctggcggtc atcgtgtcgc tgacggcgca gctcttttcc gctcccctcg tcgcctgcta
    54781 ttttcaccgt gtgccgcttc ttgggccggc ggccaacctg gtcattgtcc cgctcgtgtc
    54841 ggcggcggtc gtgggggtgc tggcgactct cacgctcgat ttcgtctggc cgccgctcgg
    54901 cgctctcgcc ggcgccctcg tggaccgcct gatggatctc atcctcgggg cgctgggcct
    54961 gttctcggga gagcgcgcgc ccgtcctcga caccggcgcg ctgccgccgg ctgccgcggt
    55021 cgctgcctac gccgccctga ttgtcgccgg cttcgctctc ggatcgctcc cggtgcggcg
    55081 gcgggcggtg cttggcggcc tggtcctcct caacggcgcg ctcgcctggc tggccctcgg
    55141 cccccggccg ccccgggtcg tctgcgacat ccacatcacg cgcgtccccg gcggggtggc
    55201 cgccgccatc gtcccggccg gtgcggagcg ggccgatctg gtcatcacca acctcgccgc
    55261 ccgggactac ccggtggtcg accgcgtcct gatcccctgg ctggaacgcc tcggtcggcc
    55321 gggactcggc cgggtagtgc tgttgtccgc cgattatgcc ggcgcggccg agctcgcgcg
    55381 cttcgccgac agcctgagcg cggacaccct gtttgtccac ggccggctgc acggcaccat
    55441 gggggacatc gcggcacggt ccgggcgctg tccaccctgg cgccccttcg gcggggcgga
    55501 gccgccggcc ggcgagggcc tggcgctctg cgcctggggc gcggtgctcg ccgcccgggg
    55561 cctcgaaatc ctgttccttc ggaccgacgg cgatctcgcg gccgcccgct gcgcccccga
    55621 ccgcgcctgc tggctggtct gcggcgatga agcggccgcg gacgacctcc gctcgatcgg
    55681 ccgcccgccc gcgggtctgg tctgtgcaga atttgcacag accgaaccgg actccgtccc
    55741 cgccgccgtc tttgacctgc gccggttcgg gtccgtgcgc ctgcggctga cggccggcgc
    55801 cgccccctcg gtccgcgtgg aaaccggccg ctgaaccttt tcccgtcaag ttccgattgg
    55861 cggctcccga tacatataga gaactggcgt acccgcgctg gcggggccta ccatggtgtg
    55921 gagagaagga agaagggtcg cagatgagtc tatccggtaa cctgaaaacg gtatcattcc
    55981 ccgacatcct ccagcttctg gcgacgggga agaagacggg actgctggaa atcagaaccg
    56041 ccagcaggca gaaagaagtt gccttccgcg acggcaacat cattcacgcc tcgtcggtaa
    56101 actccgccga ggacctcctc ggtcagatgc tgttgaaccg cggccggctc tcgaagaagg
    56161 atctcgaccg ggccatcacg ctccataagc agaccggacg gcagctcggc accaccctca
    56221 tcgacatggg cgtcttcgac aagcacgaga tcgccgagtg cctcaaacag cagatcgaag
    56281 agattgtgta caacctgttc tcgtggaaag agggcgagtt cctgttccat gagaacgccc
    56341 accccaagag cgccccgttc ctcgtcgagc tcagcacgat gagcgtcatc atggagggga
    56401 cgcggcgcat cgacgagtgg ctcgagatcc agaaagtgct cccgcccgac gacgtcctcc
    56461 tggcgatctc gcggtcgccc cggactaatg aggaggagtt caaaatctcc gtcgacgagt
    56521 tccggctcat cggcctcatc aacggcgagc gcaccctccc cgacctcatc aacctctcgc
    56581 ccatgggcga gttcgtcacc tgccgcgcca tctaccgcct tattgtcaat cacctcatcg
    56641 aagcggtcgg gcggcgcgag cagaagaacc aggtcgagga ggacgaggaa gaggccgtgc
    56701 tccgcatcgt cttccggctc tacaacaatg ccttctaccg catccgcacg ctcttcgaag
    56761 agtacctcgg cgaggacaac accactttcg ccaccttcgc cgcccagtac cgcggcaacc
    56821 tgctgaactt cttcccgggc gtcgacccca agtccgacct catgccctcg ttcgacaagt
    56881 tcctcgccgc ggtccaggcc attcccgcgc cgattcggtt ccagacgttg ttccgcgacc
    56941 tcgatgacgc cctggccgaa cagctccagt ttatcttcga actgctcggc gtcggggcct
    57001 atcgcgacgg cgtcacgacg gtcaagggcg agatttccga gccgctggcc acccggcgcg
    57061 acttggtgaa gcgatacgcg atcgaagaaa atttcaatca ggccgtgaaa cggtctgccc
    57121 gcgttgtcaa aatggtgaga gggtgaatat gaaacgcacg attatgtccg ctctgctgct
    57181 ggccggggcg ttggtaccgt tctcgcgcgc cgaggccgcc aacatcgccg ccattatcaa
    57241 cccgccgacc ttcctgaatc tcctggtcct ggtcggcgcg gtcgtgtgta ccgccggcgc
    57301 cgtgcaggtt ttctcgctag tacgcggcgg ccgcctgagc cgctgctggc agtggttcat
    57361 cggcggtttc gccgttctgg cgctggccga aattattttg ttgtgtggcc tgtttgaact
    57421 gctgacattg ccggagttcg ttgtgccaac gttacttctg gtcatggccg ggctgttctt
    57481 gtacggcgtg cgggaaatca aacgaacgtt gagctgacgg acattcaagt tgacataact
    57541 tgctgcaatc tctatattta gtgagcagaa aatgttgtcg accgaaaaga ccttgcagca
    57601 aagagactta cggacacagt agatgaacag taccaccgag ctggacgaac ggatatccaa
    57661 gtgccgcaag atcctcgaag aggatcccaa ttcgcagatt tttgcggcgc tggccgaatc
    57721 gtatcgcaag aagggtgaac tcgacaaggc cttccgcgtc tgtcagaacg gcctgaaagt
    57781 ccacccctcc tacggctcgg cacatgtggt catggccaag atcaacctcg accgcggcct
    57841 ctacgactgg gccgaggtgg aggcgaataa ggccatggag ctggacgggc gcacgcgcac
    57901 ggtcgagctg ctgctcgcgg agatctacat ttacaaaggg gagttcgagc gcgcggtcaa
    57961 actcctgagc cagctgcacc aggccgaccc gggcaacgcc cagatcgaac gcctgctgga
    58021 aattgcgcgc aaactgcccg aggaatcgcg cgccgtcacc gagccggccg ccccggcgcc
    58081 cacggctgcc cccggaccga ccgccgccga acccgatctg cccctcccgc tggcggttcc
    58141 gcagatcctc gacgaggcgc ggaccatccc cggcgtgccg ggcgccctct tcgtcaattt
    58201 cgagggtctg gtcatcgaga cccggtggca gctggacatg gaccaggccc gctgtggcgc
    58261 caccatcggc gaactcggca acgtgctgaa cgaaagcctg ctcgaaggct cctttggggc
    58321 cgtccgctct atcctcgtgg aaacggcagg ccctaccttc tacgtcatac gggagcagga
    58381 cggcgtcttc attttcgtgg gcgatgagtc ctgcaacctg ggaacgctgc ggatgagaat
    58441 agaaaagctg gtggcccgct accagcccta gctgcgaagg acacgttatg gacaggaacg
    58501 gacgatacag ccggccgatc gtgctgctgt cgctcatcgt gctgattatc ccgatggtgc
    58561 ttttcccgaa ccggctggga accgatctgg cgcgcgcctc gctcatctac ctgatgtacg
    58621 agctcgcctg gtatctggcc gccgtcttcg ccctcacgcg gcgcaccagt ctgctgccgc
    58681 tggcgcaggc ggccggcatc tgcctgctct accgcctggc cctcggcctg gctttcggcc
    58741 tgatgatcgc cgtcgcctat gccatgaacc tccgggtttc gctgacactg gcgctctcga
    58801 gctacctccc ggcggtgctg ctgcacgtgg ccgcggcgcc gttcatcctc atccccgtgg
    58861 tccggcgcgt ggtggcgccg gtgcatccgt ggccggtgct ggtgaccgac cgacaggagg
    58921 gcgactcgcc tatgcatgac gtcaagaaga cccacaccgc cggcagcccg cgcggcttgg
    58981 cggccgcgag cgcggcgccg gccgagtggc ggtccgaccc ggagcgccac ggccgctcag
    59041 aatccgaacc cgcacccatg cggccgggcg acgccaacgg ctttgaccgc gccaccagct
    59101 acatcggcga aaaccccgct gtccgcgccg ccgccgtcgt cgatatcgag ggccttctgc
    59161 tcggccattt ccagcgcggg ggagacctgg ccgaagactg ggcgcctctg gcccgcctct
    59221 tttacgaaca caaccgggac gtcgccgccc gcttcagcgg cggcctgccc cagcgcctcg
    59281 cgctcagttt ccccgatcgc aaaatcatca tcgcccggga agagacgttc tacctcatgg
    59341 tcgtctccga acaccaccat gacgagctcc tgaacgtccg cttctcccaa ggcctcgaga
    59401 tagtcaggaa atatgttgcg gaacggtacg gccaggagcg agccgtgaac gcggagaatg
    59461 tatatgtacg aaattcttag tgagctcaac aagaccagcg gggtcaccgg atccatggtc
    59521 gtcgggagcg acggcattct catcgccgcc gacctcgacg cctcgttcga aggagaaacg
    59581 gtcggggccc tggcggcctc gatcaccagc aatatccaga agagcctcca ccgcctggac
    59641 ggccatcctc tccggcaggt caccatcgaa tccgaggagg ccaagctgtt tttcaccgat
    59701 gccggtctgg gcatcctggt ggtcacaacc gagaaggacg tcaacgtcgg actgatccgt
    59761 ttggagatca aaaacgcgat cgaacgcctg cgcaccgtcc ggtaaccgca tgccgaccaa
    59821 cgacgacata gggacgagac gacatggtat cgattaatta ctccgcccgc gaagtgtgct
    59881 gtaagattgt ctactatgga ccggggttgt cgggcaagac gaccaacctc cagtacgtcc
    59941 acgccaaggt gccgggcaac acccgcggca agctgatttc gctggccacc gaagccgacc
    60001 gcaccctcta cttcgacttc ctgccgatca acatcggcac catcaacggc ttcgccgcca
    60061 agtttcagct ctacaccgtc ccgggccagg tctattacaa cgcgacgcgc aagctcgtgc
    60121 tgcgcggcgt cgacggtctg gtcttcgtcg ccgacagcca gccgagcaag atggacgaga
    60181 atattgaatc cctgaccaac ctcgaggaga acctccgcga gtacggctac gacatcggcg
    60241 agctgccggt cgtcatccag tacaacaaac gcgacctccc cggcgtcctc cccgtcgctg
    60301 aactcgagcg ccagctcaac ccgcgcggct ggccgtcgtt cgaggcctcg gcgaccgtcg
    60361 gcaacggcgt gttcgacacc ctgaagatga ttatcaaaat ggtgctggag aaggccaaga
    60421 gttcgggcag ttcgcggccg cgggcgatca cgccgatgga ggaccgggtt gctccggcgc
    60481 ccgaggctcc gcgggtggac gatcgcgagg aggcggccgc gaccgagacg ccgcccgcga
    60541 cggccgcgga attcgcccgc cagggccgcc cggccgccgc cgaggacccc gagtctgtgc
    60601 agtaccgtcc gtaccccggt tcgcgcacgg gcgcggcggc cgagagtgcg cctgcggcgg
    60661 tcaccacggc cgagccgccc cggccttcgg cgccgtcggt cgccgccgcc ggcagcctcg
    60721 cccggacgcc ggtctcggcc gcgaccggaa gcggcaccgg cgccgtttcg ccccacatgg
    60781 cgccgcccct gcggcgcaag aaagaggaca agaagcgcgg cttctttaag cgtctgtttg
    60841 gattgaaata gccacccagg aggcaggaat gtctgacgat agtctcatta tttacgaaga
    60901 agaaatcaac cagattgacg cgctgatctc caagatgctc aaaggagccg aggcgaaatg
    60961 cgccctcctg gttgacaagg acggacacct gattacccgt caaggcttca cccactcgct
    61021 cgacacgacc gccctggccg ccctgctggc cggctcgttc gcgtccacta aggaaatcgc
    61081 caagcttgtc ggcgaacccg agttctccgt gctcttccac caggggaaga aggaccacat
    61141 ccacatgtcc ctggtcggcg agcgctcgat cctcgtcgtc attttcgacg accggacgac
    61201 gatcgggatg gtgcggctct acgccaagga gacgtccgcc gagctggcca agatcttcga
    61261 caccatcaag gccacggccg cggaaaacaa acagcaacag gcgaaattcg ccaacgagtt
    61321 tgcccaggga gctcaggaca agctggacga catcttccag gactgagaga ctccgcccct
    61381 gcggtggtgc ggcggaggga cgacacggga ctgcatgggt aaacagcaca tgaggtgctg
    61441 accgaggaac tatgccttca gacatcgggg caatgctggt caaggccggc agaatcacac
    61501 cggaacaggc cgagaaggcc gatcgaatgg cccgcgaaca cggcgagaag ctcgaagccg
    61561 cgctcgtccg catcggcgcc gtcgagtcgg aagaggagat cgccaagttc atcggcaaac
    61621 acctcaagat cggcgccatc cgcctcgagg acatcgagct caaccccgat atcgtcaagc
    61681 tcatccccgt cgacatcgcg cgcaaattca aggtcattgc cgtctccaag ctcaacaaga
    61741 ccctcctcgt cgccatcagc gacccgaaca acatctacgt cctcgacgcc gtcaagttca
    61801 tcaccggctg catcgtccag cccgtcctgt ccccggagag ggcgatcgag aaggcgatcg
    61861 accagtacta cgccgacggc ggcgggctgt ccgaaatcgt caaggggctc gaggatgccg
    61921 agggactcga agtggtggag gccgaagacg gcccgagcga catggacctc atgtcggcca
    61981 ttcaggacaa gccgctcgtc aaactcgtcg actcgatcat cggcgacgcc atccgcatgg
    62041 gcgcctccga catccacatc gaaatgtacg agaagcgcat ccgcgtgcgc taccgcatcg
    62101 acggcgacct gcgcgagatg gccccgctcc ccttcaagta ccgcgccgcc atcgtctcgc
    62161 gtgtcaagat catggccgac ctcgacattt ccgagcgccg cctgccccag gacggccgca
    62221 tcaagatcaa gatctccgga cgcaccgtcg acctccgcgt ctctgtcctc ccgaccatct
    62281 tcggcgagaa ggtcgtgatg cgtattctcg atccgaacgc cctcatggtc gacatgaccc
    62341 ggctcgggtt ccgcgaggag gacctggcca agttcgacaa ggccatccac ctgccctacg
    62401 gcatcgtcct ggtgaccggc ccgaccgggt cgggtaagac gaccaccctg tactcggccc
    62461 tcaagcagat caacaccgtc gacgtcaaca tcatgaccgc ggaggacccg gtcgagttca
    62521 acttcgacgg tatcaaccag gtggccgtga aatccgacat cgggatgacc ttcgccgccg
    62581 ccctccgctc cttcctgcgg caggaccccg acatcatcat ggtgggggag attcgcgacg
    62641 gcgagacggc cgagattgcg atccgcgccg ccctgaccgg gcacctggtg ttttcgacgc
    62701 tccacaccaa cgacgccccg tcgtccatca accgcctcat cgacatgggt gtcccgtact
    62761 acctggtcgc ctcggccacc aagctcatcc tggcccagcg gatgatgcgc aagatctgcc
    62821 agaactgcaa ggaggagatc aacctctcgc ctgagcaggt cgagagcctc ggggcgccga
    62881 aagaactcct ccgcaacatc cgcgccttcg aggggcgggg gtgccgcgag tgcggggaca
    62941 ccggatcggc cggccggacg gcggttttcg aggtcatgcc gatcaccccg gaaatcgagc
    63001 ggctgattct taaccgcgcg cccgacaccg acatagccca gaaggcgctg gagcagggga
    63061 tgttcagcct gcgcatgtcg gccgtggaga agatgaaagc cggcctggtg tcgatcgacg
    63121 aagttttcgc ggtcaccacg aacacctgat ctctccaaat ccgcctgtcc gccaaccgct
    63181 tatcgagccc gccacgggcc tttccggtca agattccgcc tcgcccgccg ataatagtac
    63241 agaagatatc ggcccgaaaa aacgaatgcc ggcctcatag attgggagat cgagatgttt
    63301 acactccgcg agctgctgga agagatggtg aaactcaacg cctccgacct gcacctcacc
    63361 gtcggctcgc cgccggtcgt gcgcgtcgac ggccgtctgc agcgcctcaa ctacgatgtg
    63421 ctgacgagcg agcagaccaa gaagctgtgc tactccatgc tcacggagaa gcagaagctc
    63481 aaattcgaac agaactccga gctcgatttc tccttcggca tcgagcagat gagccgcttt
    63541 cgctgcaacg tttttatgca gcgcggcaac atcgctgtcg tcctccggca gatcccctac
    63601 aagatccgca cgttcgatga gctcggtctg cccaaggtga ttgcggaatt cgccaagctg
    63661 ccgcgcgggc tcgtcctcgt gaccggtccg accggctccg ggaaatccac cactctcgcc
    63721 gccatcatcg acaagatcaa ccgcgagcgg cccgtccaca tcatcaccgt ggaagacccg
    63781 atcgagtacc tgcaccgcca ccaggcggcc ctggtcaacc agcgcgaggt ctactccgac
    63841 accacctcgt tcgcgaccgc gctgaagtac gcgctgcgcg aggatcccga cgtcgtgctc
    63901 gtgggcgaga tgcgcgacct cgagacgatc gaggcggctt tgtcgatttc ggagaccggc
    63961 cacctcgcct tcgccaccct gcacaccaac tccgcctcgg aatcgatcaa ccgcatcgtc
    64021 gactccttcc cgaccaacca gcaggagcag gtccgggtgt cgctctcgtt ttcgctccag
    64081 gccatcgtgt ctcaggcgct cataccgaag atcggcggcg ggcgggtcat ggcgctcgaa
    64141 atcatggtct gcacccccgc gatccgcgcc ctcgtccgcg acgacaaggt gcaccagatg
    64201 tactcgatga tccagtcggg tcagaagtac ggcatgaaga cgatgaacca gtcgctctcc
    64261 gagctctacc tgaccggcaa gataaccctc aatgacgcga tgagctatag ctcgaacacg
    64321 caggaactcg gcgaaatgct ctcccgccag aaatctccgg cgttttcgta atgtgagtat
    64381 gggaaaggac gtttgccatg ccagtattcg agtataaagg caagacgctc gccggcgccg
    64441 ccgtccaagg ctcgctcaaa gccaagagca agggcgacct cgaacgggtc ctgcggcaga
    64501 accgcatcct ggtcacctcc atcagcaagc gggccccgga aatcagcctc cggatcggca
    64561 ccggcatcaa gcggatccac gtctcccgct tcacccggca gtttgccacc atgatcggcg
    64621 ccggtctccc catggtccag tgcctcgaga tcctcgccgc gcagtcggaa aaccgcgagc
    64681 tcgcccggat catcaacgat gtcaaagagg gcgtctccgg cggcgcgact ctctccgagg
    64741 ccatggcccg ccaccccaaa atcttcgacc ccctctatac caacatggtc gaagccggcg
    64801 aggtcggcgg cgccctcgat tccatcctcg tccgcctcgc cgtctaccgc gagaaggccg
    64861 acaagctgat tcgcaaggtg aaaggagcga tggtctaccc gagcgtcgtc atgatcgtcg
    64921 ccatcggcgt caccaccgcc atgctcacct tcatcgtgcc cgtcttcgcc aagatgttcg
    64981 agggactcgg gtcggaactg ccggcgccca ccaagatcgt gctccacatc tcgcatttcc
    65041 tccaggccaa cttcgtcttc ctgacgatcg ggctgattgg cctggtcgcc ttcatcctgt
    65101 ggtggcgcaa gacgccgcaa ggcgggctgg tctgggacaa gtccatgctc cgcatgccca
    65161 tcctcgggac tctcgtacgc aagtcctcgg tcgcccgctt tacccggacg ctcggaacgc
    65221 tgctgtcttc gggcgtctcc atccttgacg ccatggacat caccgcccgg accgccggca
    65281 accgcgtcat cgccaacgcc atcaacaagt cggtcatggc catcgctgag ggcgacacga
    65341 tcaccgggcc gctcaaggaa tcgggcgtgt ttccgccgat ggtgacccag atgatctctg
    65401 tcggcgagaa gaccggcggc ctcgacgaaa tgctcaacaa gatcgccgag ttctacgatg
    65461 aggaagtcga cgacgcggtc tcggctctga cctcgatcat cgagcccgtc atcatcgtct
    65521 tcatgggtat cgtcatcggc ggcatcctca tcgccatgta cctgccgatg ttcgacatca
    65581 tcgggaagat cggctgatcc cgagatcccg ccgccggcgc tgggccggcc gcggacaccg
    65641 aatgaccctg ctggacggcg ccctccgcgg cgccgtccgg cgtgcgggaa accatggtta
    65701 gcgccgaaca cccccgccgt ctcgcgtggt tcatccccct ccggttggcc actttcctcg
    65761 tggtcttctt cgtcgtcgtc ttctggatga aattccccgg tttcctcgcc ctgccgttct
    65821 tcctctacgc cggcgtcacc ctcggcttca cgctcctgct ggccctggac aagcagcagc
    65881 ggctcggcgc cgtgaccacc gccatggtgg cgctgcactt cctgttggag atcggcgtcg
    65941 aatccggcat catctacgcc acgggcaacg tgaactcccc gttctcggct ctctttgtcc
    66001 tgacgatcgt ctcggccgcg cttgtctacc gtctggtcgg taccctcgtc atcgcctcca
    66061 ccgtcgccct ggcctacgcc ttcatcgtct ggctcggtct gaccaacagc gatcccgagt
    66121 ggtcgctccg ggcgctgcgc acggtcttcg acacccagga aaccgccttc tactcgatct
    66181 ttctgcacct gctgattttc tatctcgtcg ccttcatcgc cggctacctc ggccagcgcc
    66241 tgcgcgacca ggatcgccgc ctcgccgacg cctcccgcgc ccttcgccgc gcccgcctgg
    66301 agaccgacga tatcctgcgc cacctcaatt ccgggctcat cacgattgac gccgagggct
    66361 gcattgtcta tttcaaccgc accgccgagc gcatcctcgg gtattgcgag gaggacatca
    66421 agggtctcag ctgcgaccag gccttcgccg accgcatgcc ggccctggcc cggagcctga
    66481 tggacggcgt ccggcggcgg gtcgcccacc tgcgccagga gctcgagatc atcaatccgc
    66541 tgggccgccg cgttcccctc gggctgtcga tctccattct gaccgaggag gacaaggcca
    66601 cgccgcgcgg cgtcatcgcc atcttctccg atctcaccga ggccaaggac ctggaggcca
    66661 aggtccgcgc ggccgaccgc ctcgccgccg tgggcgagct ctccgcctcc atcgcgcacg
    66721 aaatccgcaa tccgctcgcc gccatctccg gctccgtcga ggtgctcaag agcgccctcc
    66781 cggtcgcggg ggagaacgcc cgcctcatgg acctgatcgt caaggagtcc gaccggctga
    66841 ccaagatcct cagcgaattc ctcctctacg cccgcatcga ccggcccagc tacaccaagg
    66901 tggacctgtg ccgcctgacc agcgacgtcc tccatatcct ctcccaccac gagtcccgcc
    66961 accccggcat ccgcctggct ttcgatgccg atcaatcggt ggtgtacgtg gcgggagatg
    67021 aagacctgct cacccaggtg ctgctgaacc tcggggtcaa cgcctgcgag gcggtcgggg
    67081 ccgctcccgg cgacgtacac ttccgcgtct tctcccgcga tccggagaga gtcgtgctgc
    67141 aggtcgccga caccgggtgc ggcatggcac cggaagtcat gcggcgcatg ttccagccgt
    67201 tcttttcgac caagcgccag gggaccggcc tgggcctggc gattgtccac cgcgtctgca
    67261 ctgccctcaa gatcggcctc gccgtcgact ccgctcccgg ccggggggcc acggtcacgc
    67321 tcgaattccc ggtgtgcatg ccgcggcccc agctccaggg cgccgcgacc gcctcggcgg
    67381 cccccgccgc cgtccgctga ccgcccgatt ttggttggct tttctccgcc cgttgtataa
    67441 cttaatcagc caaccggatc ccgtgtgatc gcaaccgtga cggagagatg atggccttac
    67501 gaacctatgc ccggctgacg ggcgtcctcg cactcgtgat cgccgcctgc ggcgagccgc
    67561 cgacgctcac ccacgaatcc gtcgaccgct ttgaccgggt cgtcgcccgc gtcgataccg
    67621 tctacaccct caccttcccc gagttctacc ggaaactgga agagagcatt ctgctgcccg
    67681 acggcggccg gctgtcgcag cgggaggtgg ccgacgaact ggatcgcgtc gtcatggaca
    67741 cgctcgccgg tctggtcgcc gacaccgtca acctcggcgc caactacgag ctcaaccgca
    67801 tctaccgcca gcggtactac gagtaccttc tccgccgttt tggccaggtg gctttctttg
    67861 aaaagacctc ggtcgattcc gcggccgtgc ggcagtatta cgaacagaac ctcgcgcagt
    67921 tcacgacccc ggaggaggtc gacgtttcgc acattctgat cgccgccggg aacctgcccc
    67981 acgaccgcga ttcgctgaag tatcgcggcc tcacccagcc ggaactggcg gcggcggccg
    68041 agaagcatgc ctggtacgtg aaatccctgc tcgaccaggg tcatcccttc gattccatcg
    68101 cccgcgagta ttcccacgac aacctgatcg cttcctacgg cccgcggatc ggctgggtcc
    68161 ggcggggcgt ctatgaacac cccttcgatt cggtcgcttt cggtctcgcc ccgggccggt
    68221 actccgatcc gtatttcgcc tcgaacagct ggcatcttat ttacgtcgcc gcttatcact
    68281 ccgacaccac cgccccgctg gaggggacga tcgccgacca ggtcaccgtc catctccgca
    68341 cggcggccgc cgactccctc gcccgtccgt tcctggactc gctcgatgcg gccatgcagc
    68401 ttgaaatcaa cgaggccatc cttgacagca atgtcttcct gttgcccaag ccgctgtggg
    68461 cggcggtggt caacggccgc gatacggtcg acatggccga cctccagagc ctcgaggagg
    68521 gattccggaa gcgccaccgg gtggacaaca ccacccccga catgaagcgg gagatgatcc
    68581 agattgccgc ccggcggtat gcgctggtgc aggcggcccg cgccgccggg atcgaagaac
    68641 tcccggacgt gagggagcag gagttcaagc tccgccgcca gtaccagaag acgatcgtcc
    68701 tgcggcaggg gctggatgac ggctggacgc cgtccgacgc cgagatcgag gcctactatc
    68761 gccggaacta ccgcgagacg ccgatcgaca aacccctcgt cgtccagcac atcatcgcgc
    68821 ccgactccgc gtcggccgct ttcctccgcg accaggcgct cgccggccac gatttcctcg
    68881 ctctcgccga gcagtactac cccggcgatt cgagtctccg ccgcgacctc gccgacctcg
    68941 ggccgatcgg gccgcaggac gtgtccccgg aattctatga ggcggcccgg gtgacgcccg
    69001 atggcaacgt cagctttccc gtccaaaccc agtacgggtg gcacgtcatc aaagtgctgg
    69061 agcatcgcac gccgataact ctggacaaag tgcggacgca gatccggcag accctgcgcg
    69121 atcagcacct ccgcgagatc accacgactt tccgcgaccg gctctaccgc cgcttcgccg
    69181 tctccttccc gaatcgcctg ccggcggttg acctccgccc catacgcgac cggcaggagg
    69241 ggcaggatgg acagtgatgt gacagtctcg gcgccgccgt ccgctccgcc gccgatcagc
    69301 tttcgcagcg agctggcgcg caaagccacg cacatgttcg cactcgtcat cccggccggc
    69361 tactacgttc tcggtctggg gcgcgggacc atgctcgcgg tcatgatccc ggtggccctg
    69421 tgcaccttct ttgtcgatct cgcgcgcctg caggggcgcc cgctctggcg ggacatcttc
    69481 gggccgcttt tcggccgcat gatccgggcc cacgaggcgg ccggggattt caccggtgcc
    69541 acctacatcc tcgcctccac cgtcgccacc gtggccctgt tccccaaagc ggttgcggtt
    69601 gccgccctcg ccttcaccgt ggtcggcgac agtctcgccg cggtgatcgg gcgacgcttc
    69661 gggcggcgcc ggtttctcca cggcaaatcg gtcgagggct cgctggcctg cttggccggc
    69721 acggtaatcg tggctctact cactccaaat ctggcggccc cggccgcgtt tgccggcgct
    69781 ctggccgcca ccgtggtcga ggccctcccc cttcagatcg acgacaacgt cacaattccg
    69841 ctgctgtcag gcctggtcat gaccctcgta ttgcgtattc tgggctgaaa atcggccggc
    69901 ccgccctttt ttgcttgtct gccggggtcg aaatcccgta taattaagtg aaggctgaca
    69961 tatctgtcat aacccgaacc cgctagcgga gggtctatgt ccaagggtac tgtgaagtac
    70021 tttaacgatc tgaagggatg gggcatcatt tccagcgcgg attcgcccga ggacatctac
    70081 gttcactaca cctcaattaa catgaacgga ttcaagacct tgcgtcaggg acaggaagtc
    70141 ctgttcgacc tgattcacag caatgggcgc cccctcgccc aaaacgttct tccctcgcgc
    70201 tgacttgttc tgcatcaccg acttctgaaa ggccgtgccg ctggaacacg gccttttctt
    70261 tttccccccg cgtcttgtcc gggccctccg cgctccctat attcccgccc atgaggaaga
    70321 tcattgatct gcgctccgat accgtcaccc gcccggtccc ggccatgcgg gcggcgatgg
    70381 cggccgccga ggtgggcgac gacgttttcg gcgatgaccc gaccgtgaac gagctccagc
    70441 gcacggtcgc cgatttattc ggaaaagaag ccgccctcta tgtcccctcc ggcaccatgg
    70501 gcaaccaaat tgccctgaaa gcccagacgc ggcgggggtg ggagctcctc tgcgaccgcg
    70561 agtgccacat cgtcaactac gaggtcgccg ggccggtagt ccatagcggc ctgctggtga
    70621 atctcatccc gaccgaatac ggcctgctga cggccgaggt ggtccgcagc cacatccgtc
    70681 ccgtcaacct ccacaccccg cgcaccaagg ttgtcgcgct cgagaacacc cacaaccgtc
    70741 acgggggaac catcctgccc caggacgaaa tcctcagagt ccgcgaggtg tgcgatgagt
    70801 tcgggctcgt attccacctc gacggcgccc gcatctggca tgtccacgtc gccaccgggc
    70861 gcccgctggc cgaactcgtc gcgcccttcg actcggtgtc ggtctgtctc tccaagggtc
    70921 tcggggcgcc ggtcggctcg cttgtcctcg gttccgcggc gctcatcgac gtctgccggc
    70981 gcgagcggaa gctgttcggc ggcggcatgc gccaggccgg catcgtcgct gccggcggtc
    71041 tctacgccgt gcggcatcac attgggcgcc tggccgagga ccacgccaat gcccgctatc
    71101 tcgccgcggc gctcaacgag ttcaagctgt tcagcgtgga cctggcccgc gtccagacca
    71161 acatcgtcct ggccgacata aacggcgcgg agacctccga ctccgtgctc gcccggatga
    71221 acgaggtcgg cgtgtgggcc atcgccttcg gcctcaaacg tattcgcttg gtcacccacc
    71281 tcgatgtccc gcgcgcggac tgcgaggaag ccgtcgcgcg gctccggacc atcctcgtat
    71341 gaccgcgccg cgccccgccg acaaactcac cattctcggc acctcggccg gcatgcccca
    71401 gcccgaccgc gccggctccg gctgcctgct gacggtcaac ggccgccact cgctcctcga
    71461 ctgcggcggc ggcatatgcg cctcgttcct gcgaaggggg tgcgatccgc tagcggtcga
    71521 ccgcgtcttc gtcacccaca cccaccccga ccactgcacg gaactccccc tctttctcca
    71581 gatgctgcac ctcgccggcc gcaccgaccc ggttgagctg tttgtccccg atgagttcgt
    71641 ggaacccctc cgcgcgcttt tggccgccat gtacatcccg agggagaagc tccctttcga
    71701 accgcggctg tgcggttaca gcgacggctt ccgctactcc ggcgatttca cgctcacggc
    71761 ccgcgccaac gaccacctcg tccacaaccg ggagctgttc gcccgcctcg gcctgcccaa
    71821 ccgcatgcag agccattctt tcgacatcgc agtcggcgag cggcgcctcg tgtacagcgg
    71881 cgaccttaag gcattcgagg aaattcggcc ctgggtggac ggctgcgcct gtgtcctcat
    71941 cgaggccacc cacgtggatc ttcccccgct cgtcgagttc gcccgcagcg ccgccgtcgg
    72001 ccgatggatc ttcacacacg tccagagcgc ggcccacgtc cgcgatcttc acgccgccct
    72061 ccaggcggcc ggtctggaaa actacgtcgt ggcggtcgac ggcatggagt tcccgctctg
    72121 atcgacgggc tcgggccggg caggggagag gcgcggtcac ggggccgccg cgggtgcgcg
    72181 tgccaaaaga aaaggcaggt tcgccggaac ctgcctcgca tgtattgccc ggaccgccgg
    72241 tcagccggcg ccgcccgtcg ctttcgcgcc ggcccgggag gccgccttgg acttgtacag
    72301 ctcccggttc atctgcgcaa tgaaccggac ggggatctct ttc
//
LOCUS       DAOPOE010000005        61270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_631_length_61270_cov_5.343658, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000005 DAOPOE010000000
VERSION     DAOPOE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 61270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61270
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_61270_cov_5.343658"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKM94_01445"
     CDS             complement(<1..611)
                     /locus_tag="PKM94_01445"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HOD65330.1"
                     /translation="MKNAVALVCTLVILAAGSAFAAQVTNVGLTWQDGYTVARIDVQG
                     AVRFSHQPEPAKDGRPFRVIVDVLAATHQLGQKDFFELPACPVTKIRTSQFAVQPEQV
                     VRLVFDMTRESVYKIASDATGITVSFPDPEGKQFAAWSSKTWLVGQAKPAPPAVAQAP
                     AEARPTMPPPPSHKTTAEVNQSINTDRAASLANPEQAAPPAAVA"
     gene            complement(628..1248)
                     /gene="pilO"
                     /locus_tag="PKM94_01450"
     CDS             complement(628..1248)
                     /gene="pilO"
                     /locus_tag="PKM94_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOD65331.1"
                     /translation="MDFKDSKTQKIALAVLAFFIVAYFWYSRLYVPYDSKIELKSQEF
                     ETITTNLRNVELKAKSLDALQQEYTDLMKRYHEIEALLPEVKQIPSLLVQLHTASSLT
                     GTRITRVQPRAIRPEDFYNVASFDIEMTGTYHDFGGFIGYVANFPFIANVSGVDVNAM
                     KLMVNKPAKDEKTGLTDLSKKETVTAKFSLSTYFVKESERLSELVL"
     gene            complement(1261..2025)
                     /locus_tag="PKM94_01455"
     CDS             complement(1261..2025)
                     /locus_tag="PKM94_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD65332.1"
                     /translation="MIEINLLPKGYRKKQASFSFGKTGLYAVVAAAGVVLMLVGVTVY
                     QMNQLSSLEDGIVKARQRAAMLEKDIKLVDALTDVKAKISARMAAVERLDSHRSSWVR
                     ILEDISANVPEFVWLARFTEKAPLADTAKMAAAEAGAPAGQPAPAGQAGQPNAAPATT
                     TPSVPKVMPVEVEGYAFTLNALASFMINTMRSDYFEEVELVSTKETQLGEYRAYNFVL
                     SAQLHYLSEEELQGLVAQAEAEQTEQAAPARHKSLN"
     gene            complement(2033..3085)
                     /gene="pilM"
                     /locus_tag="PKM94_01460"
     CDS             complement(2033..3085)
                     /gene="pilM"
                     /locus_tag="PKM94_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOD65333.1"
                     /translation="MPFARASKSTVGLDIGASSIKLVKLDHTKSGYAVSAIGIRELPP
                     EAIVADEIRDREAVIFNIQSLIDQTDPKIKNVVISLSGHGVITDRFTIDKKTGAEAEQ
                     AILFETEQRAPFDVDDVSLDYHVVKVDDAANKMDVLLVAARKEFLQMQMEMIEDCGLR
                     PVVVDDDALAIYNAYQYNYEVDPSKVTALVNIGADVTNLTYLVDGLYHSTRDVSAGTR
                     EIFNAVQKEFRLNPELTSKAIKGEMTDSIDQDMFKATIVSASDELLAGIELAFSYFKS
                     QSRVDTIDWIVLSGGGALTPYLPELLQSKLSIPLEIANPLRKIDYDPELFQYIQPEKI
                     APLLAVSVGLAMRKVR"
     gene            complement(3347..3853)
                     /locus_tag="PKM94_01465"
     CDS             complement(3347..3853)
                     /locus_tag="PKM94_01465"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOD65334.1"
                     /translation="MKNLRTSSRGITLLEMMVTVVIIGIVASLAVPRFDGAMDRIRFR
                     SVNRDLTSTLRLARSSAITDKDQYGVYFDGTHRVVYLFKDLVSPTLNQFDTGDSVLRA
                     DTLPPEFNFLGTDVVGDVICFAPNGSARFTGGGNVVCLASTEAMVGIASHNILASTGR
                     VQSSTSYY"
     gene            4223..6604
                     /locus_tag="PKM94_01470"
     CDS             4223..6604
                     /locus_tag="PKM94_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD65335.1"
                     /translation="MSRSRLRDLLTCAVTTAALVAAVAFSASAADRPAMLLTDQPVVQ
                     AKAAPVLSDRARAYLAGQAADTVGVWVFFTDKGIRTKDEFSARAASVAMTDKVLQRRA
                     KVDRDRVLFADLPVAAGYVDAVVAAGGHHRRTSRWLNAASFDLPAADLAAVAALPFVA
                     EIRPVAVFKRSLPDLWEVRSDGPGSAALAPDALNYGYAQAQLEQINVPAVHEKGLDGS
                     GVTLAVFDTGFRKTHEVFANAFAEGRVLAEWDFVFNDGNTDYEPSVDWSSSTSHGTLT
                     WSTSGGYKSGRIIGPAYRANFLLAKTEDVRSETSVEEDNWVAALEWADTLGADVITSS
                     LGYSDWYSYSDMDGQTCITTIAAATAAGLGIVVVNSMGNEGPGSGTLTAPADAHPIIA
                     CGAVDINGSIAGFSSRGPTYDGRMKPEVVACGVSTSAATNGSDVSYGTASGTSLSTPL
                     VAGAAVLLVQAQPSFTPEMIRLALMQTADRASSPDNTYGWGLIDADAATSWGVSMAAD
                     STINNGPLKVHFTGTSALGPTSWLWRFGDGDSATVQNPAHTYSNPGAYDVSLTVETSE
                     FGQLTTSRPHFIIILGDTAHYGSDSTFAGSDLVVSINVTNSQPLARLVVPLTFGSAAW
                     LTFDSVTRGDRTAYFERLNPLNSNDLTREYVYELLADFGGGSPPLAPGSGEVLKLWFS
                     TDELALGGQSILIDSIASPYVISFEAGYLTYSPTVAAGTGALIDVMRGDVNYDHDLSI
                     ADVTHLVAHFYRGGPPPVCLQAGDVNRDFMMSVADLTYYVAYLFRGGPPPPTP"
     gene            complement(6695..7795)
                     /locus_tag="PKM94_01475"
     CDS             complement(6695..7795)
                     /locus_tag="PKM94_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD65336.1"
                     /translation="MDPRTEKLAQVLVHYSLRLKKGELLKIQGEYVTLPLMTAVFEEA
                     VRIGAIPYVRVIIPQHDEIFLKKGTDAQFAYISPIARTEVNTMDVFLHIWGTANTRHL
                     SGVDPKRQALQRKCQRPLSDKLFKRMETKSLRWCGTLFPAQAEAQEASMSLAEWENFV
                     YRAGHVTSGDPVKHWTKVKKEQDRLVKILNRTDRLHIRSADTDLKMRVKGRKWINCAG
                     TENFPDGEVFTCPIEDSAEGVIRFSFPAIYVGREVEDVRLELKKGKVVKESAAKNQAY
                     LTGMLNMDKGARYVGEIAIGTNYDIDRFSKDILFDEKIGGTLHMALGRAFAEAGGKNV
                     SALHWDMICDMRKDAEVLADGKVIYRNGKFTI"
     gene            8066..8416
                     /locus_tag="PKM94_01480"
     CDS             8066..8416
                     /locus_tag="PKM94_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65337.1"
                     /translation="MNESAVYVSPSTARSLWQEYRIYERHVEFDTHIGTVTIPFDNIE
                     SVEVQGSDVKALLRGDLRLKGFKPAIKLDWANFTEHVVLDKKTGACKRFLFTPQDPHE
                     FKAVLDRLMAKHRG"
     gene            8688..10244
                     /locus_tag="PKM94_01485"
     CDS             8688..10244
                     /locus_tag="PKM94_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65338.1"
                     /translation="MRFLLWLCIAVLSSPCAGLAAGPVVIDTLISDTVASWFGMSIVP
                     IGDQNNDGYDDFLTFELPRKLYLCLGGPVETGITITRIDTAGSPLIGNLGDINGDHIT
                     DFGCPIRLSSIDSYRLGVWYGGSPLDDGPDLVFGDSTYFPGVSAPVLGNDINGNGTLE
                     LISSHSDNWDYPLLMFFELGGHADSMPDLLLRAPGTNMAMADGVITGDFNGDSFDDLV
                     TNMRSILNDSVKGEVLLYWGGPGFDTIPDARITRRGEYSWLYDTFGTRLESLTDVNGD
                     GWDDFFIHYRGGSGDSLSWIHFGGPAFDTVPDVILTTRNQKARLAGDVNHDGYADLIT
                     GYATSFSGLGYADIYLGGPDFDSIPDVVIDHSMFSGYYNYFGHEVAGIGDVNGDGIDD
                     FAVASSRGSGYPWVIFIFAGWDDAVSVDDTLDPEPALPTNFTLDQNYPNPFNPITTIS
                     FSLPRAETVTLRILNALGQTVAVLINEQRLGPGEHAVVWEAEAYASGVYLYELEYPAG
                     KQSRKMVLLK"
     gene            complement(10315..10491)
                     /locus_tag="PKM94_01490"
     CDS             complement(10315..10491)
                     /locus_tag="PKM94_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65339.1"
                     /translation="MKNGGFIFQNHRALQEVREKINTSQANPVNAGLTEQPEGWPWIK
                     LPLLRRLAECLAGH"
     gene            10896..12245
                     /locus_tag="PKM94_01495"
     CDS             10896..12245
                     /locus_tag="PKM94_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65340.1"
                     /translation="MPFVDTDTTTYPKKFWMGHKQQLSHPVRSIYPRITAQDSILCVT
                     YSSREAVTKAGTWWVYRVLSFDYGLTWSEPRAIENTGDFNAGDLFPLIEGDTILLSFS
                     RQVEGIETTYQQVVAKESLKDAALTTPVVLQQSTANLHHSQLVRHQDTTWLFYVFSDW
                     DYNHLASCRSTDNGQTWDTLNFSGPSIGWPMSFQRANDHISWVGEEGVESYCRLLNPD
                     GVTWTDRVLISETPANTASQWPNACSNGLSDLHAGWFDFDGAEGGWYGYIFYTHSYDG
                     GESWGPIWSLSPTDDCEHSAFWADSQRVYAVWNDCRENGSPDFALYLRYSHDRGMSWS
                     PEIEVVDKIDPAREPDLYGQGDLVYLVWNEQHPPDWIWGIYFQVGGWYIPGDVNRSED
                     LTVSDLTFLVSYLFRGGAAPVLLGAAEMNGSAPINIADLTYFVDYLFRGGAEPVGAQ"
     gene            complement(12450..14612)
                     /locus_tag="PKM94_01500"
     CDS             complement(12450..14612)
                     /locus_tag="PKM94_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOD65341.1"
                     /translation="MTETPLAGLPAAESFTLLDLIPIDELQHVQDSLAAATGVASVIV
                     DLSGRCLTRPSNYSPVCRLVLASPVGRRRCALSSEQLLGRARRQPGPTHHRCLSCGFV
                     DAAAPIIVEDRHIADWLIGQCDAHRVGSDRIRAFAREIGLDEEALAAAYERVAPIALD
                     QFERHLDLLWNVAHELSELGYRNLCYRRELAERRQIEELLRRREGENHTLLDAMPDLV
                     FQLSRGGVLEKCHGSREDMYRAPEQFIGKHVSEVLPPDVADITIQNLRQAAATGAVQV
                     YEYSLPIGGRTRYFESRLLMGSPDSALAIVRDITERKRAEQEQARLHERLEKAERMEA
                     LGILAGGMAHDLNNMLGPLVGYPELILAKLPPDSPVRKYIESMRKSAVEASEVIQDLL
                     TLSRRGRYDMTPVDLNEVVQACIDSAPFRNLTSRKPQVAVTLRLCDSVTAMNGSFPHL
                     QKLVGNLLANAFEAIDTAGEVTVTTARRCLETLPGGYTRIDPGEYVTLSVRDTGRGIP
                     AADLDKIFEPYYIKKMVGAGGTGLGLSVVYGVVKDHGGYYDVFSEPQRGSEFVVYFRT
                     IQSPVAARPPVGDFSGRGENILVVDDAAAQRDLASELLTGLGYRVAAAENGRQALRYL
                     DTHVADLIVLDMIMEDDFDGLDTYREILLRRPDQRTVIVSGYSSTERVQEMQRLGAGA
                     YIRKPYSLESLGRAVREELDRAVGDGAPSVLAAADPLR"
     gene            complement(14719..16770)
                     /locus_tag="PKM94_01505"
     CDS             complement(14719..16770)
                     /locus_tag="PKM94_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOD65342.1"
                     /translation="MSVSNLIVETSLVSAASAEEAHRRQNLLGGTFEGHLLRLKALTE
                     EELVGAIRSGAGCDAIALSGLPIPESVIGYLPRRFAEQRCLLPVAFDPAANRLTVACL
                     DPRDEGLAAEIRAVTGVQEIHLAAALEPVLRCRIIEAYRNRPEVDDTRGAAGPDAPPA
                     PRRPILLVGAIDPAVETLARALALEGQSVILVETVDEALAEIAAAAPRAVVVRSRPNE
                     THAVLEDHLRATAPQCPVCFAEGVADLITAAPPAELTGSLFTAALAMWPAGAAPADEL
                     KVRRVAERVDLTAQLCDRLKLSPRDRLLVVHAAIAAGAPERPPSDRRPPEVAAILAHR
                     ESEIADTYADRLPLAVLGGSILRLVDTLADRWGQLKDPTPELYRALTAELRPLRGQAF
                     LPQVVDAFIAMVSERIPTPRPFDRASTQALVLGDADGYVGALAGALAGAGFEVLRAGT
                     AARFIDLYGSRWPDFLVIVSSGSARDARRMVDDLADRGIAFDTVPALLAVPTRVIRDL
                     ADLLDSRIEDILPIEAGPELGEARLRRLRLRLEAEHSRQMSMLQSMGTHGSLADMNLI
                     DLLQALGYSQRTVRISVTACGAQLTMYLDGGRLIWAECGDTVGAEAVYLGLGWARGIW
                     SVDPVGRESLPEPNSHLPIDALLIEGCHRLDEGGGPGTVRYEELPAELPPNGDLGLRE
                     T"
     gene            17112..18734
                     /gene="pruA"
                     /locus_tag="PKM94_01510"
     CDS             17112..18734
                     /gene="pruA"
                     /locus_tag="PKM94_01510"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOD65343.1"
                     /translation="MYRIPQPKNEPILNYAPGSPERAALKEAIKFLKANPIDVPLVIG
                     GQEIKTDHKIKITAPHDHNLLLGHYYQGGEKEVKMAVEAAVEAQKSWQHMPWEQRSAI
                     FLRAADLISTKYRYIMNAAAMLAHSKTAHQAEIDAVAELADFYRFNCWCAQQIYDTFQ
                     PESSPGVWNRIDHRPLEGFVFAVTPFNFISINGNLPVAPAIVGGVSVWKPASTATYTS
                     HWIMKILREAGLPDGVVNLLFAKGRDIGELVLKSRHLAGIHFTGSTPVFQMMWKTVGD
                     NLPNYNTYPRLVGETGGKDFIIAHPSADVDAVVANTIRGAFEFQGQKCSAASRCYFPE
                     SLWPTIKQKMIAELGTIKMGDVENFGNFMGAVIDGAAFKSITEYIDYAKQHKDKCEIV
                     FGGRYDDSKGWFIEPTVVATTDPHFRLMVEEIFGPVLTIYVYKDAEYANTLHLCDQTS
                     PYSLTGAVFARDRMAVALAERTLRHAAGNFYINDKPTGAVVGQQPFGGGRASGTNDKA
                     GAIQNMMRWISPRTVKENFIPPTDYRYPFLKE"
     gene            complement(19160..19948)
                     /locus_tag="PKM94_01515"
     CDS             complement(19160..19948)
                     /locus_tag="PKM94_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65344.1"
                     /translation="MKLLMRLILLSLSLVCSGGSAGMDDVKWDVDQDTVMSDEQPLPE
                     PVLLIERFQLYVYMECEPDHEVLRNTGRMGIDIRTAYRTVGFDAITTDSSLTLRLRTL
                     NRGKREDRVSLPAESFVLYVTLRSDTASYWVERTDTGFSITSRRQSNWLELGDVRTLE
                     ANVRWMQFARDATQGDVKEFVKQLRENFADIVTLVQIAPGYYPCLPAPVVQSSTIGRR
                     LRANKTAAGYLVGFQVRGEPVDVQRVRNWVMQQQTQELGRSGKN"
     gene            20537..21139
                     /gene="rdgB"
                     /locus_tag="PKM94_01520"
     CDS             20537..21139
                     /gene="rdgB"
                     /locus_tag="PKM94_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOD65345.1"
                     /translation="MQLVLATNNRDKIREIRHLLEDLPITILTAGDFLDFPEIDETGE
                     TLEANALLKARGIAAFTDLPALADDSGLEVDALGGQPGVYSSRYAGDNVTYDDNNRKL
                     LEELAGVPRAERTARFRCVIAIDWADGTHETVEGVAEGWIAEDIAPGNGFGYDPVFVH
                     SETGQRFSAMRLPEKSAVSHRGQALRRAREAISARLKARS"
     gene            21297..24488
                     /locus_tag="PKM94_01525"
     CDS             21297..24488
                     /locus_tag="PKM94_01525"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOD65346.1"
                     /translation="MKKKTRTPSQLALLILVCFFLSGFTGLIYEVLWTRMIVKIIGGA
                     PFAISIILTIFMGGLGLGSYLAGRVVDRVKQPEELVRLYGYLELMVGAYGLVLPGLMA
                     LFRPLFAAMYNGVFEYGMIYNLLTFAGCALLLILPVTFMGATLPVLSRFYVSRLGHLG
                     THTGRLYGLNTIGAGFGSLATGFWLIAWFGMWGTLATAVALNAAIGLTCVLVGGRARI
                     TRGRPAEAGATGPAEVAETPPRAVISGALVIFAVSGFCSMSYEVIWTKLLGLIVGPTT
                     YSFTVVLVTFIIGLALGAMLFGWLADRTRKPAPLLLVTQVAAAAAALGVSQVLGNSQL
                     FFTKLIATFQDNFTALNAAKAALLFLFMLPPTLCLGATFPLVGKIFTPSTARVGRSIG
                     FAYAVNTIGAVLGSFLAGFVIVPALGKANGLSLVIAIQLAGALVFGWVLLRAGAVRRR
                     IWLPAAGAAAAALVLVFAYPRWDHRLLALGKYHRIADQGVKADQVGWWSALLRGPEIL
                     APLFNADVVYYGDGVAGTTTVLKTRDAMGNEDLSMTNAGKPEASTRGDMPTQTLLAHF
                     PLLFGNEAKQVMVVGLASGVTAGEILRYPIERLDIVEISREVVTASEYFRPWNHGVLD
                     DPRARLIVQDGRAHLAMTAAEYDVVISEPSNPWMAGLATLFTRDYFTYAREALTEGGM
                     FVQFIHTYQMDWPTFAMVGRTFADVFPNALLVATSPDTASNDLMLVGIKGSGPLNLAR
                     AREMGQYCRQGGNFAVRDPAVLVRLVRAQNLKELFGGGPLNTDSHPRLEYAAPKLLYR
                     SDPEIAARIAAGGRLTEPLARLKEKVATEVESQIDFLEYELSVHLPMHGFVPPILAYD
                     RATPAQRARHDSLLCAYCRQNPVVLSLCQDERARESCAACQIEGLEATVAAGRATAMT
                     YSYLAFLYSRGGQTDKLPALYREWVQSDPREAKAYHGLAAALIERNNLTEALPALLTA
                     DRENPGNPVILTDLGYLYGRMGRPDLARDYFGRALQADIRYARAHMGMGAAEATAGNI
                     PAAIAHYREALRLDPALEGAQTALAKLQAGRQP"
     gene            complement(24540..25445)
                     /locus_tag="PKM94_01530"
     CDS             complement(24540..25445)
                     /locus_tag="PKM94_01530"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOD65347.1"
                     /translation="MSTYMKRFVYMSLLYLAAGALCGILSAGGSPGYAAVFAHTHFNL
                     LGFMAMIVFGIGYFILPRFNGAELRWPAWVPVHFWLGNVSLVGMVLFRGLYSRDGSNL
                     WHVLFLAAAAVQAVSLLMFIVNIWVTLSLKKAPAGAAAAPSAPLSPSISLTEDTRIAD
                     LIERCPSVREVLVGQGLKMLDVPGHLDKVRKVGVTLGMAAANHGLDLQTLIRAAEDEV
                     NRPPSPARSAASSAAPFDITSETLIGAVLERHPRTNVIFQRYFGDGCLDCPGQSYESI
                     DLACRMHGVDPELFLAELQAAAREA"
     gene            25648..26415
                     /locus_tag="PKM94_01535"
     CDS             25648..26415
                     /locus_tag="PKM94_01535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOD65348.1"
                     /translation="MGSETGKIGKTVGAGNARVATGLTVALAIAVAAVILPKLALSGA
                     VARILTTQALELGLSLGAIAVFGRGRFANYGFRLPRPDRHWSAALAGWMPIGLTALGL
                     GTLATAAMLACGGKGNPLVRELTFPQVILFVWIFSSTIEEVFTRGFLQSHIASAGEPR
                     LGPRFLRIGRAALISAAFFAAMHLVLLLSHTDAVTVVIILLFTFSVGLLAAHQRERTG
                     SLVPAIGVHMLANVGGVFGGIVYAIIAMLSGGAVPGR"
     gene            complement(26452..27849)
                     /gene="asnS"
                     /locus_tag="PKM94_01540"
     CDS             complement(26452..27849)
                     /gene="asnS"
                     /locus_tag="PKM94_01540"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOD65349.1"
                     /translation="MDYKVRTRIRRIVVDEQVPVGTTVTILGLVRTIRRSKEVAFVEV
                     NDGSCMQNLQGVVSDPDRIPAIDRILTGAAVRLRGKLIESPAQGQKYEVAVEALDLVG
                     EADATYPIQKKRHSFETLRDMAHLRPRTNTFGAVNRIRSRLAYAIHTYYQERGFYYIH
                     TPIITASDCEGAGELFRVTTLPLDQPPKKDGRVDWEADFFAAEAYLSVSGQLEGELLA
                     AALGDIYTFGPTFRAENSNTARHASEFWMVEPEMAWAELEDDMDLAEDFLKYLFRFAL
                     DDCAGDMAFFNQRIDHKLIETLESIVASEFVRVSYTDAIAQLQKSGRKFEFPAEWGLD
                     LQTEHERCLTEVVYKRPVIVYDYPLAIKAFYMRVNDDWKTVRAMDVLVPRVGEIIGGS
                     QREERLDVLQKHMEMKGLTNWENYAWYFDIRRYGSMPHAGFGLGFDRAVMYITGMQNI
                     RDVLPFPRVPRWAKF"
     gene            28237..29166
                     /locus_tag="PKM94_01545"
     CDS             28237..29166
                     /locus_tag="PKM94_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD65350.1"
                     /translation="MSTQDSSPRSDSGADSAAGHGSPLAAEVLRLLYERASCRNFAQR
                     EIPDRLLDEILQAGVHAATGGNLQPWSIIVVRDPEARRKLAELNENQTFMQDAPVNLV
                     FCIDWRRLERWAAGAAAPFTATAAFRHFWISFQDTIIAAQSIGTAADAVGLGSVYVGT
                     VLECFPEVREMLALPPGVFPVVILTLGWPVHRPAPRGKLPLQVVVHREQYRELPDDQL
                     AAAYETKYGGAKIDCTPDRLDRLVEVCRRTGGEELAERCRARVRAAGSINMAQRYFGL
                     HYPADFMPAQNASYLRIFEDAGFEWFREWWPRE"
     gene            29410..32076
                     /locus_tag="PKM94_01550"
     CDS             29410..32076
                     /locus_tag="PKM94_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD65351.1"
                     /translation="MSSRAAVQRVAVALAAVLTAVLGAAQPGWGLPNAPVNPGSPPMS
                     DQNNSEVSLSETTPGEVYAVWTEYTPAGFGVSNIGWGFSNTLGAAWVQALIPPTPPYG
                     AEWNPSLASHPAGAFYAAAAAYGPGMPWLAPDAVVVHTSAGGGAAFGPGVPVAVNVPG
                     TTWLDYPDIALADNPAIPAPLFGTMHTAWVEYADGTFGDADGNGNPFDDAGDNYTIFY
                     AYSRTMAGPAPIFPAFSVPVALFPGPVFPNAPAANRPSVAVMAPPGNPVIPGGGVYVG
                     WTDGLNVYVTGSPALGAAFFAPALVAAITPVPPVLIGGVKGAATVTIATGAGPCAGWV
                     YAAWTSVGGADLDIFFSSSPTGLPGSWGPPVRVNQDPIGNGADQWAPHMSVDPGSGRI
                     LITYYDRRVDPANVAHQTWVSSSPDCGVTWLDCQLSTTPPAAPISTFPLPPAPIYLGD
                     YLGSDFNLLNGPAHIWNDGRAPAAVQDVYFENVMTCFPDTDGDGVFDPYDNCPTVPNP
                     GQADGDGDGVGDVCDNCPAIANPGQSDGDGDTFGDVCDNCPTVANPTQLDGDSDGIGD
                     LCDNCPGTPNPGQLDGDGDGVGDVCDNCPGVANPGQADNDTDGLGDACDPDDDNDGVL
                     DPSDNCPLISNPGQSDIDADGIGDVCDADADGDGLLNGDELTIGSNPLSADSDADNVN
                     DSVEVYHCGGGIGSPCDTDGDATPDVMDPDDDGDGINTINEDTDLDGNPRNDDIDSDG
                     VPNYLDPDSDGDGIGDISDNCPYVSNPGQVDTDTDGLGNACDPDDDNDGILDGADNCP
                     LVANPGQEDADGDGIGDACDGCCTLRGDFNDDGAVKVSDLTALINYLFRGGAAPSCLD
                     HGDTNADGSIKVSDLTLLINYLFRGGPPPAAC"
     gene            complement(32177..32248)
                     /locus_tag="PKM94_01555"
     tRNA            complement(32177..32248)
                     /locus_tag="PKM94_01555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32214..32216),aa:Lys,seq:ttt)
     gene            complement(32353..33633)
                     /locus_tag="PKM94_01560"
     CDS             complement(32353..33633)
                     /locus_tag="PKM94_01560"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOD65352.1"
                     /translation="MAKKNKIVLGCQWGDEGKGKIVDLLAAQADVIARFQGGANAGHT
                     IVVDDRKYILHLIPSGIIHSGKACYIGNGVVLDPFGFIEELDFLASRGIDYAGRLWVS
                     PAANLVLPYHKLIDAVLESSRGTASIGTTMRGIGPAYIDKVARNGIRVIDLFHPDRLR
                     KRLEYQATVKRQYLEGSSDERADLGRTFDALMEIAPILRPLVTDVSLALHRAYRAGRK
                     ILYEGAQGAMLDVDLGTYPFATSSNTTVGGALTGLGIGPHMIDEVIGVVKAYTTRVGA
                     GPFPTELVDNAGDLLRTRGDEYGATTGRPRRCGWLDLVQLRHAVRINGVTSIAITKLD
                     VLDDFDEIPVCTGYRLGDEQFDQVPLDLADLARVKPEYRTLPGWRTRTTGTTVFDHLP
                     AQARNYLNFIARDLGVTIAIVSTGARRDETIAVG"
     gene            complement(33617..34246)
                     /gene="yihA"
                     /locus_tag="PKM94_01565"
     CDS             complement(33617..34246)
                     /gene="yihA"
                     /locus_tag="PKM94_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOD65353.1"
                     /translation="MATAPTITTCDFVGSFVSAGQAPADRRPHIAFAGRSNVGKSSLL
                     NRLVGRRKIARVSSDPGKTRALNFYLVNSRFYFVDLPGYGYARVSRQMRAEWGRLLES
                     YLNGDPHLAGLVLLLDARRDPTEQDFQLVAWLAARGIPTLVAVTKTDKLNRDQTNRKI
                     AAVERLFNAPALAFSAVSGLGKNELLGAIFDLVNERAQDIGAPRHGQKE"
     gene            complement(34425..36830)
                     /gene="lon"
                     /locus_tag="PKM94_01570"
     CDS             complement(34425..36830)
                     /gene="lon"
                     /locus_tag="PKM94_01570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD65354.1"
                     /translation="MSTFKIVHNGELVTVNSRLCVIPLRDVVVFPHMIYPLLIGREFT
                     VKALREAMEGDRQVLLLAQKVAGVDNPGQEDLYDVGLVARILQVMKMPNGTFKVLVEG
                     LVRACVTSYVRTEDYVQVRISVQPPEEPAHDRELEALSRSVMELFTEYVHLNRRVPDE
                     VLVALASIEQYHQQADTIASHLLVRKEAKQEILELFDVRKQFLELARLLKEEIEILKI
                     EHKIDGDVRESMSRSQREFYLQQQLKAIKDELGQFDEPGAEVDDLYSQLESRDYPETV
                     VEKADEEIKKLAKMHPYSAEAGVIRGYVEWLLALPWNDVTQDRVDFKEVRAILDGDHY
                     GLEKAKQRILEHLAVIRLAGKVRGPILCLVGPPGVGKTSVGRSIARALDRKFVRMSLG
                     GVHDESEIRGHRRTYIGAMPGRIIQGLKRAGSANPVFLLDEIDKIGTDFRGDPAAALL
                     EVLDPEQNNSFSDNYLELECDLSNILFITTANTVATVPPALRDRMEIIRLPGYLDFEK
                     AAIARQFLLPKLVEQIGLQGVAVDFQDEALSLLIRQYTRESGVRELERQMGALLRKAA
                     VEVAEGRKLRRLTVTKRKIYALLGAPKYVDNNIKAEPTIGYAVGLAWTEMGGEVLPVE
                     VAPMAGKAKLTMTGSLGDVMQESATAALSYIRNHAAMFKLKENFFEDIDLHVHLPEGA
                     VPKDGPSAGVTLLIAMLSSLTETPVRTDVAMTGEVTLTGDVLAVGGLNEKLLAAKRMG
                     VLQVILPEKNRKDIRELPADLLKGLKLHYVRSVTEVIRLAMTRNPITRTRVRATYRIA
                     RGA"
     gene            complement(36985..37935)
                     /locus_tag="PKM94_01575"
     CDS             complement(36985..37935)
                     /locus_tag="PKM94_01575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD65355.1"
                     /translation="MEQSVPIQILHYALGERLAPSRTGDLYKAWDTVLERFVALKLFD
                     LDLMDHIPYRAQVLSTLRALADTDHPNICSLYGVHKFHGRYVVALELVEGESLRERLR
                     RGPLDIDAFLRVASALAGALNHAHSRHIVHGSLTPSNILLRDDGDVRVVDFGFSTMIV
                     EREGRGFSPSAEAVRYRAPEQVTGEEVGPLADLFAAGAILYECLAGCPAFPGSSVEAV
                     EEAVLHADPDFRLLQPDRKTPADIVLLLERLLAKNPADRLRHAAELQITLTEIVSFEK
                     AAPAREFFQVAPQSPRQYLMLSLLAALLIIFWLVITSVHS"
     gene            complement(38038..38556)
                     /locus_tag="PKM94_01580"
     CDS             complement(38038..38556)
                     /locus_tag="PKM94_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65356.1"
                     /translation="MKRLVLVVLCVFALALIATAQDEEKGYRIGVRFEAGAYQVLVPG
                     GTWSNGPVSYTINIPKGLPFTSLEWFNNDATDAVRVMGTANPTPWSPPNCVMQPGQPG
                     CQQFIYPQYPGLYSFNVGSPKGDRLDATVNITVVANASVPSLSTYGLIILLVLLGASA
                     VIVIRKRRQPAI"
     gene            complement(38615..39283)
                     /locus_tag="PKM94_01585"
     CDS             complement(38615..39283)
                     /locus_tag="PKM94_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65357.1"
                     /translation="MVCHGGWSGCELFAVGGFSALLVLVARVSPAYGCFVLVAAAPLL
                     AAVARASVRDAACLFALFALTLVGVGQLDLIRLSPLVWGLRMAAAGAVGCAFGLLFGL
                     ANRRFGFHPLVLALLWVGFELTIVQTGIAPSVLGLHAPSGAVTLELSQLFGFAIVSLL
                     IILLDSAILWSLEKIFSAEARGTNERVNGRIWNLLFTPRPLAGRFFLVPHRRGPPPAT
                     SPQH"
     gene            complement(39751..40257)
                     /locus_tag="PKM94_01590"
     CDS             complement(39751..40257)
                     /locus_tag="PKM94_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65358.1"
                     /translation="MRRYLILLVGAACVLLACGRGVETIAEFPVDNLDGIIAQSNVTL
                     DREITADGGGALRIEAPAAATIPLLELVDPDVEKATLTFEAKIRTQNLTGMVYLEMLC
                     HFPERGEFFARGLDNPVTGDVDWSSQSTQFFLKAGENPDLVKLNIGVAGSGTIWVDAI
                     KVTRGPLK"
     gene            complement(40378..41016)
                     /locus_tag="PKM94_01595"
     CDS             complement(40378..41016)
                     /locus_tag="PKM94_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOD65359.1"
                     /translation="MAERRQTNEEIEIKLDLGSFTNYLKLVGFLGQLDGEERQVNAFF
                     DTEDRVLSRDGWALRVRVDEQRGSLAIKSRETAGEEASIRMEMESEIPRGEALEALAL
                     QRDIMGMDTPTIQFVRDQFGSLALVRFVHFENTRQYKAFRFGDYSYRLEIDTTRYADG
                     SVDYELEVELPDRTHVPTVIDRLRKLFLSLDIPFLRQNRSKLARALERAPRA"
     gene            complement(41154..42692)
                     /locus_tag="PKM94_01600"
     CDS             complement(41154..42692)
                     /locus_tag="PKM94_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOD65360.1"
                     /translation="MKKVILVNSTDYETRVALLEDERLVELQVERPDSDRMVGDIYKG
                     IVRTVLPGMQAAFVDIGLPRAAYLHASDVGKDYDNRYDSEDIDEEEAPAEIIRKRKRE
                     TIEQVLKTGQEVLIQVIKEPISTKGPRISTEISIPGRYVVLVPDDDHIRMSKRITSWA
                     EKRRLKKVLAPLRPEGFGLIVRTEAEGHDTPDFRADVKRVLKLWSKLKRKADTSKAPV
                     LIHKEAEMIVSMIRDLFTDDVQRLIVDNKGDYKKVISYARQVVPHLKNRVELYKGQRP
                     LFDQFNIEAEIERMMDRKVWIKKGAYIIIDQTEAMVTIDVNTGRFVGSSDQETTILQT
                     NLLAAKEIARQIRLRDIGGLIICDFIDMYSKENRRKLYEEFKGCFRNDRAKGAINPVT
                     DFGLVEMTRERVRPSHLQALSEPCPTCEGLGRILSKETMATKIERWFLRAKAEGRIRE
                     FNLALNPMLADAMSENGHSRIDRLMRINQFRINVVRDTTLSVQEYRVYDAQNNDNITE
                     RYRL"
     gene            complement(42655..43653)
                     /locus_tag="PKM94_01605"
     CDS             complement(42655..43653)
                     /locus_tag="PKM94_01605"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD65361.1"
                     /translation="MRLVFMGTPEYACPTLACLVHSEHEVAAVVTGPDVRPPRGGGLH
                     PTPVKREALRHGLTVLTPATLRNNPALREALAGLKADVFVVVAFKILPRSLYALPRLG
                     SVNIHASLLPRYRGAAPIHWALINGETETGLSSFVLNDTVDTGDLVLQERVPIDPADT
                     FDSLYARMAALSGPFALRSLERLADPAFAPIPQDGTAATPAPKVTPEDALIDWGFPAP
                     LVHNFIRGLSSVPGAYSTFRSRRVKILGSRPVVDAPAAPGAPPGAIVPDRRRLLVQCA
                     SSALELTRWCRRGRRGWTGCPFSTACSRSRASDSEPHRTTFRIPYEESDSRQLNRL"
     gene            complement(43732..44241)
                     /gene="def"
                     /locus_tag="PKM94_01610"
     CDS             complement(43732..44241)
                     /gene="def"
                     /locus_tag="PKM94_01610"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD65362.1"
                     /translation="MAVRPIVIYGDPVLREKAQPVETIDREIKDLVSDLTDTLKEAQG
                     LGLAAPQIGVSRRVFIIDLSVIDINADLKVFINPEIIEAGGEPVEYEEGCLSFPGLYQ
                     KITRPAWVKVRAIDINGNAFEIATDGMLARAIQHEFDHLEGILFIDRMSALTRTMIQG
                     RLRKLQKVS"
     gene            complement(44256..44627)
                     /gene="yajC"
                     /locus_tag="PKM94_01615"
     CDS             complement(44256..44627)
                     /gene="yajC"
                     /locus_tag="PKM94_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOD65363.1"
                     /translation="MRFPRGPADRTTGEGVETHVNWPILMAGSPEGAGSGTMITTVIW
                     FGLIFVVMYLLLIRPQRKKQKEHEHLLKDLKKGDKVITSAGMFGVIFAIDDERGRVVL
                     KFGENVKMEFLKSSIAARVDS"
     gene            complement(45211..45687)
                     /locus_tag="PKM94_01620"
     CDS             complement(45211..45687)
                     /locus_tag="PKM94_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Ni]"
                     /protein_id="HOD65364.1"
                     /translation="MLRTFVSAALVLGVLAFGSGSAAAHCEIPCGIYDDSTRFDLLAE
                     HITTMEKSMAQIRELSADPGANANQLTRWVVNKEDHAGEFQHIVSQYFMTQRLKTLEP
                     TAQGYAGYQKQLELTHRLLVGAMKCKQTTDQANIDSLRVTLDQFRTAYFGGQNHTH"
     gene            complement(45977..46366)
                     /locus_tag="PKM94_01625"
     CDS             complement(45977..46366)
                     /locus_tag="PKM94_01625"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOD65365.1"
                     /translation="MSNGVTDILKGVVGELREVAKSETVIGDPITVGDRTVIPVMKIS
                     FGFAAGGGQGTDEKRGSGFGGGGGGGARVEPAAFIIIDRDEIRVLSAVKGKWDALLEA
                     IPEVAKKIQQAAKKFRSDEKPADSSGA"
     gene            complement(46383..47024)
                     /locus_tag="PKM94_01630"
     CDS             complement(46383..47024)
                     /locus_tag="PKM94_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65366.1"
                     /translation="MPWWPVVIGLLLLLGSGVLALSVRVRLVFEEGRRLIGLSLGRRT
                     GIEVDAGARAGVVRLAGIPLKKFRFGAGAAAPKEPAPAAPRRKRSPRTAGGARRDLAE
                     IGRLGPRILRALGSYLAALSRAATVEECRAHIEAGFASPDHTGLAFGCYQAALGAGPV
                     WLAQIRYVPVWDGPAFRASGRFTAALPLYRLAGRTVLLAWRLPIRKIIRLARA"
     gene            complement(47041..48765)
                     /locus_tag="PKM94_01635"
     CDS             complement(47041..48765)
                     /locus_tag="PKM94_01635"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOD65367.1"
                     /translation="MIGTRMLWALAAALFLCGPAPAAAAAEPGLDNLCDQILAALQAF
                     YPVRSTEMGIHDYDHRFTDYSSSSVRDMVKRLAAFDKKLAKYRAADLPADQAIRCRLI
                     QSNVDAALLDLKQISWHTRLPQLYVDEAVNGLYLLLGSRSLAPEQAAVSVLARMKAVP
                     ALLETARKNIRRPPQVWIDEAVSALVSAEVFYGDAGAEMARQFPARAEEFRTAAAAAQ
                     EVLRGFSDWLRRAEPGDPGAFAIGADNFNYKLAHEHFLPYTADSLLALGEALLVDADS
                     AYDEYRRFVEEEAQNGSERVFIPRCFSRADILDYYAWEVAQVRAFCADNEIVTVPQNI
                     APVAVVETPPYLQSMITGIAYQPAGPFDTAQQALFFVRPVPDPLDSAQLSVRARYIHR
                     RGFRGSVVHEAYPGHHLQTQVAGMHDDPVRKWQSNSLAVEGWALYCEQMMYEAGLYGG
                     ENPAMWLAILDGIRFRAARIVADVKLHTGRMTYDECVDWMIDTLDIDTDSGREYIRNQ
                     VRLYTHRPTYQMSYLIGKLEILKLRDAMIDIEGESFSLRAFHDAYLSEGSIPPALVWK
                     AWELETAP"
     gene            complement(48852..50204)
                     /gene="rlmD"
                     /locus_tag="PKM94_01640"
     CDS             complement(48852..50204)
                     /gene="rlmD"
                     /locus_tag="PKM94_01640"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOD65368.1"
                     /translation="MAEQEVELVVTDLAFDGKAVAHRDGKVVFLNGGLPGETVLAAIV
                     KSKPRYDQAVVRRILTPSPMRLPARCSHFGDCGGCTWQDLDYRQQLVFKRKQVADCLT
                     RLGRLEQVAVDEMIGCAELYNYRNKMEFSFHTAPGGFTLGLHRRGQFDDIFDLDRCYL
                     QSELSNRIVRWIRDFVVRTRIPVYDVANHTGYMRFLVIRQGKRTGQTMVNVVTNYGDF
                     PDRQRLVRDMRAELPEVTTLVHNQNGRISNIAVGESEQVLFGLGYIEEQLFDCRFRIR
                     ANSFFQTNSIQAETLYRTCFEMLRPGPSERVFDLYCGTGSIAILLAGYVQHVVGVELV
                     KDAVDAAVENAACNNVGNVEFHHADVKDYLALIEGSSVRFDTVIVDPPRTGLHPKALR
                     RLLRLDPPRILYVSCNPATFARDARLIVDAGYDLPLVRPVDMFPHTMHIELAGVFNRR
                     "
     gene            complement(50213..51136)
                     /locus_tag="PKM94_01645"
     CDS             complement(50213..51136)
                     /locus_tag="PKM94_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOD65369.1"
                     /translation="MAHTHPPASRTTSGARLGWAILFNTAITAAEIVGGLISGYLALL
                     ADAVHNLSDVAALGLAWLGWRGSRLPATKRSTFGFLRVEVITALVSAVALVVVAVFIL
                     IEAYRRFLAPQPISHPVVFLTVAAIGLLGNLFSIWILHAERSSSLNMKTAFLHMFYDA
                     LSSVVVIAGGLVMLATGWYLVDVILSAAIAVMIFWSSWLVIREAAMILLEAVPAGIEY
                     DAVIAALRADPAVVGVHDLHIWSLSSREVALSCHVCVDPGDLARGPEVIARLSRGMQE
                     RFAINHSTMQLETEECGRLDANGMCRRPEIH"
     gene            complement(51228..51896)
                     /locus_tag="PKM94_01650"
     CDS             complement(51228..51896)
                     /locus_tag="PKM94_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOD65370.1"
                     /translation="MTGIVTAPLRWMRALYDWVIRWSESRHARTALFFLSLAEASFFP
                     IPPDALLIALCMGAYRRWAKFALICSLGSVFGGMLGYLIGHSAFELIGSHILSWTASL
                     SGSDPEALLQTARYWFNEKELWGMKVGAWAVGIAGFTPIPYKVFTIAAGFFEMSFPVF
                     VIASVLSRSARFFLVAGLIGLLYRRHGRRIQVFIDKYFNALAIAFVLLLAGGFWAISL
                     MKGD"
     gene            complement(51915..53222)
                     /locus_tag="PKM94_01655"
     CDS             complement(51915..53222)
                     /locus_tag="PKM94_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD65371.1"
                     /translation="MDSLWLQIIAILVLVLANGFFALSEFSVIASRKSRLKQKVAEGK
                     SGAAAAEKIRANPDRFLATIQVGITLFGTLAGVFGGATIVVTLEQLLRNSPIEVVSDG
                     ARPIAVALISVAITITSVVLGELVPKYIALSNPERYARYVANPILVFIRIAWFFSWIL
                     TALANLVLRILGIRRDSEGQAVTEDEINLMIFEGKQSGVFDETEEQLVRNVFDFADST
                     VRRAMTPRTDVVGLPLDGDPAQVIAVVIDNGYSRYPVYEGSLDRIVGILHTRDLVLHR
                     LNAHEFALRDLIRKPVFVPDSLPLARLLRDFQRKKNKFAVVLDEFGGTAGIVTLEDIL
                     EQLVGEILDEDERGELPLVRHSESVAYADGAVRPGAVNVLMDARLPEDRAETLAGLVI
                     DHLGHLPSKDDSVIIGDMKITVLELADNRLQRLKLERLAVPTA"
     gene            complement(53280..54158)
                     /locus_tag="PKM94_01660"
     CDS             complement(53280..54158)
                     /locus_tag="PKM94_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOD65372.1"
                     /translation="MNTLKVTFMMLALMLLFMWVGYLVGGQSGLIIAFVFAAGMNFVS
                     YWFSDKIVLAMYRARQVSESDHPRLFRVVKHIAAAARVPTPKVYIVPSKGPNAFATGR
                     SPEHAAVAVTEGLLELLDENELEGVVGHEFAHIVNRDMLVGTIAATFAGAIGMLASMA
                     RWGAIFGGYGGRDNERSGGAIGLLVAAIVAPLAAMLIQMAISRAREYKADEAGGGFTG
                     KYLPLASALDKLHRAPVKMRLDDRPATAHLMIANPLSGKGLSSLFSTHPPAEKRIEKL
                     RQLAQQSAYKGYAGAR"
     gene            complement(54327..56660)
                     /locus_tag="PKM94_01665"
     CDS             complement(54327..56660)
                     /locus_tag="PKM94_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOD65373.1"
                     /translation="MSPQTNRKILYGMLAGVLLGAVAGFVIPDVMLAIAVIGRLFVNA
                     LQILLLPLIPALIIVGVAGLAHAPRVTRAAASTVLYFLATTLAAVVIGTVLVLLIQPG
                     RGAQLTGAFVPDMVNRARAAVLPDVFASFIPDNLVAALGAGQYLGLVVLSLVFAGIVA
                     AMGARARVVVDFFKAIADISLEAVNLVIYAAPVGLFVLVGSAIARNPEAIGSLGGNLA
                     WLMLTFLLGLLLHGLVVLPVLLQFLGKRNPLAYGATTVPALTTAFGTASSAAALPITY
                     RNVVEEGGVDARAGALVLPLGASMNANGTALYLTIAALFTAQLYSVPLSIVQLIAIPL
                     LAVILSLGTASITGGALFALAVLFAALNYPTEAYNAIGLLVVVDWLFDRGRAALNVWG
                     DMVGAAVISESFEFKTARSTRGITTPERAPVRRERGAGRRERPPREHQTTEGGRDSRR
                     PPREPREGDRRGGRGRDRDHGRKPERTPQDQPPRRESEPRPAATTKQESRPASPPQAE
                     PSRGQQRRERPEPPRQPKRESERPAPPEQRPRPPAIPTGGETPAPDRGRPAAPVTPPL
                     READRRPSREPAPPVEPPAPREPARETAPDVPVPEIRRRPLPIEVRAAQRPDDRPGAT
                     PPAEAPAPESPVRAEAPRREFAPEPPPPPPIWRPAEPAEPKTDKSDDEFVPDAPATEP
                     RLREILASLAASRERLDVESADAEGEDSDEKEGPGEPEPEFRPASFGRGRARHGLRPS
                     DQTQPPEPETPEPTDRKDGYDVSAVPSFGRARRKRTR"
     gene            complement(56994..58172)
                     /locus_tag="PKM94_01670"
     CDS             complement(56994..58172)
                     /locus_tag="PKM94_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD65374.1"
                     /translation="MAACAGWVRAQPLGALGFGEDSLPALLPGDTVEIPIRLINAAPG
                     FETGGFNLFFQYDTTAFRLLSAAPGSLLSACAWELFVWRAAIPSDCDGSACPGGLVHI
                     TALADYIIPVGEPLCWELSDGELVKLTFAVSGNPAVAGQTHLFRWYWYGCNDNEFASK
                     NGDSVYQSRLVLDENAADVTADLPFPNTTGAPLWCIASSTITRRIDYAHRAIAIAADS
                     LAPVKLRFQPPLLHAFQVNALGPVVCSLYIGNLAECAITDIDAASLRLNDTLVPLGAA
                     VAPAPDGFFGDVLAVAFDARPLIAPLVALYDTATVPVAVSWSAPEPCVEEAAGELTVV
                     GHRSGDVNGDGRVSIVDLTTLVAAVFRGGPIDPVMGDVDGSRRITVADITLLIDRLFR
                     "
     gene            complement(58384..60489)
                     /locus_tag="PKM94_01675"
     CDS             complement(58384..60489)
                     /locus_tag="PKM94_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65375.1"
                     /translation="MYLPTVSLFVRRLLPVALLVGALGTAVWAGPAVIRVESIENAAL
                     ADTVELAVIMDAPHDSSALTGFNLLLTYDQTGMAFLAADAGQLLVDCQWEYFTYRSGI
                     DSTLGGSPLALVRLVAIAEINNGSVHPDCPLDTPGELARLRFRTTASPGAADHQFPVR
                     FYWHDCDDNVFADSTGDTLLISNRVFEFGQEITEEHPFPTYFGAPSACLIQSTIIRGA
                     DFYNGSVRIDAQAAPPDAPVRVVISPLEAPVLGGPVEFAAQLHQVQSGLALGGFDFLV
                     RYDPAQLTWMSAQPGNLLESCGWEYFTFAAGAQAECGQFPCPAGVARVIAVADLASPT
                     GTPTCGADTEGTLANFLFTVANDTSLIGRPLAVEWYWYTCDDNRMAVLSGDTTFISRS
                     VYDHFGQPAPADTAFPTASGAPSLCAAGPDIIPGIDFFAGWTRAHPSEIDLRGDVNLN
                     GIANEVSDYVLFGNYFFYGLAAFTANVATQIATTDVNADGLPLTLDDHVFLARIVVGD
                     AIPFPRPNAAPTDTAVIVQDGALGSVIVTYPGSLRAVYVVFDGAVLYDGPFGSMPEPW
                     PHLGGHTPLLASGGIDVDSAPFLPSTVDGLQLCTYTGVAPIVTAQASSDGVTPVPVRV
                     IVRNIPDCCLLRGDFDSSGGLSVGDVTAMVAYLFRSGPPSLCPAHADVDGSGAVNVTD
                     ATRLIGYLFRGGPAPAACP"
     gene            60803..>61270
                     /locus_tag="PKM94_01680"
     CDS             60803..>61270
                     /locus_tag="PKM94_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65376.1"
                     /translation="MDFFDHTGQSDDPEVPRETDRNIPLADRMRPRTFDEFVGQESVV
                     GPGKPLRKAIEEDRVSSIIFWGPPGSGKTTLAALIARATRGQFVPFSAVTSSIKDVKE
                     VLTKAGNYYRMSGHRTYVFIDEIHRFNKAQQDAFLPYVEKGDIVLIGATTENPS"
BASE COUNT        10815 a        18967 c        20779 g        10709 t
ORIGIN      
        1 gctacggccg ccggcggggc cgcctgctcc ggattggcca ggctcgccgc ccggtcggtg
       61 ttgattgact gattcacctc cgccgtcgtc ttgtgcgacg ggggcggggg catggtcggc
      121 cgggcctcgg ccggggcctg ggctacggcc ggcggggccg gcttggcctg cccgaccagc
      181 caggtcttgc tcgaccaggc cgcaaactgc tttccctccg gatccgggaa cgacaccgtg
      241 atcccggtcg catcggaggc gattttgtac acgctctcgc gggtcatgtc gaacacgagg
      301 cgcacgacct gctcgggctg cacggcgaac tggctcgtgc ggattttcgt caccgggcag
      361 gccggcagct cgaagaaatc cttctgcccc aactggtgtg tcgccgcgag cacgtcgacg
      421 atcacccgga agggccggcc gtccttggcg ggctcaggct ggtgcgagaa ccggaccgct
      481 ccctgcacgt cgatgcgcgc cacggtatac ccgtcctgcc aggtcagccc gacgttcgtc
      541 acctgggcgg cgaacgcaga gcccgcagcc aaaatgacaa gcgtgcaaac cagtgctact
      601 gcattcttca tcgggtacct cacttaatta cagaacgagt tcactgagac gttccgattc
      661 cttgacaaag tatgtcgaca ggctgaactt cgccgtcacc gtctccttct tcgacaggtc
      721 ggtcagcccc gtcttttcat ccttggccgg tttgttcacc atgagcttca tcgcgttgac
      781 gtccacaccg gacacgttgg cgatgaaggg gaagttggcg acgtagccga tgaagcctcc
      841 gaaatcatgg taggtaccgg tcatttcgat atcgaaggac gccacattat agaagtcctc
      901 cggtcggatc gcgcgcggct gcacgcgcgt gatgcgcgtg cccgtcagcg aggaggccgt
      961 gtggagctgc acgagcagcg acgggatctg tttgacctcg gggagcaggg cctcgatttc
     1021 gtggtaccgc ttcatcagat ccgtgtactc ctgctgcagg gcatccagcg acttggcctt
     1081 gagctccacg ttgcgcaggt tcgtcgtgat cgtctcgaac tcctggctct tcagctcgat
     1141 cttgctgtcg tacgggacgt acaaccgcga gtaccagaag taggccacga tgaagaaggc
     1201 cagcacggcc agcgcgatct tctgcgtctt tgagtctttg aaatccatag cctatcttcc
     1261 tcagtttaag ctcttgtggc gcgccggcgc ggcctgctcg gtctgctccg cttccgcctg
     1321 cgccaccagc ccctggagtt cttcttccga aagatagtgc agctgggccg agaggacgaa
     1381 gttgtaggcc cggtactcgc cgagctgcgt ttccttcgtc gacaccagct ccacctcttc
     1441 aaagtaatcc gaccgcatgg tgttgatcat aaacgaggcg agggcgttca gggtaaaggc
     1501 gtacccctcg acctcgaccg gcatcacttt cgggacgctc ggggtggtgg ttgccggggc
     1561 cgcattcggc tgaccggcct ggcccgccgg tgcgggctgc ccggccgggg cgcccgcttc
     1621 ggcggccgcc atcttggccg tgtccgccaa cggcgccttc tcggtaaagc gcgccagcca
     1681 gacgaactcc ggcacgttgg cgctgatatc ctcgaggatc cggacccaag acgaccggtg
     1741 gctgtcgagg cgctccacgg ccgccatccg ggccgaaatc ttcgccttca catccgtcag
     1801 agcgtcgacc agcttgatgt ccttttccag catggcggcc cgctggcgcg ctttgacgat
     1861 cccgtcctcc agcgaggaga gctggttcat ctggtacacg gtcacgccca ccagcatcag
     1921 caccaccccg gccgccgcga cgaccgcgta gaggccggtc ttgccgaagg agaagctggc
     1981 ctgcttcttc ctgtatccct tgggcagcag gttgatttct atcatggtcg acctacctca
     2041 ccttccgcat ggccagtcct accgataccg ccagcagcgg cgcaattttc tctggctgga
     2101 tgtattggaa aagctccgga tcgtagtcga tcttccgaag cggattcgcg atttccaggg
     2161 ggatgctgag tttggactgc agcagttccg gcaggtaggg cgtcagcgca ccgccgcccg
     2221 agagcacgat ccagtcgatc gtgtccaccc gcgactgcga cttgaagtag gagaaggcca
     2281 gctcgatccc ggccagaagc tcatcggagg ccgagacgat cgtcgcctta aacatgtcct
     2341 ggtcgatcga atcggtcatc tctcccttga tcgccttgct cgtcagctcc ggattcagcc
     2401 ggaactcctt ctgcacggcg ttgaatatct cccgcgtccc ggccgacacg tcgcgcgtcg
     2461 agtggtagag gccgtccacg aggtacgtga ggttcgtcac gtcggcgccg atattcacca
     2521 gcgccgtcac cttggacggg tccacctcgt agttgtactg gtaggcgttg tagatcgcca
     2581 gcgcgtcgtc gtcgaccacg accggccgca gcccgcagtc ctcgatcatc tccatctgca
     2641 tctgcaggaa ttccttgcgg gcggccacca gcagcacgtc catcttgttc gccgcgtcgt
     2701 cgactttcac cacgtggtag tcgagcgaca cgtcgtcgac gtcgaacggc gcccgctgtt
     2761 cggtttcgaa caggatggcc tgctcggcct cggcccccgt cttcttgtcg atcgtgaacc
     2821 ggtcggtgat cacgccgtgg ccggacaacg agatgaccac gttcttgatc ttcgggtccg
     2881 tctggtcgat cagcgactgg atgttgaaga tgactgcttc ccggtcccgg atttcgtcgg
     2941 cgacgatcgc ctcggggggc agttcgcgga tcccgatcgc gctcaccgca taccccgact
     3001 tggtgtgatc cagcttcacc agcttgatgc tgctggcgcc gatgtccaga ccgaccgtgc
     3061 ttttgcttgc tcgagcgaat ggcatatgca actcttcggt taggggagtg attcttttct
     3121 ttctctttgt atcggcacga tccgccgccg atttatccct cgtctcgcaa ggtttgatcg
     3181 ccggccgctt cttctcatat ggcaacctgt tcagagtctg atactttatc ggcggcttcc
     3241 cccaaccctt taccattatt tccggcccgt tctgccgccc gtgccaatga aaaaggcccc
     3301 cctccgagag cggaaggagg cctcgcggcc cgaccgggcc atgtcttcag tagtagctgg
     3361 tggaactctg gacccgcccc gtcgaggcca gaatattgtg ggaggcgatc ccgaccatag
     3421 cctccgtgga ggccaggcag acgacgtttc cgccccccgt gaaccgcgcc gaaccgtttg
     3481 gcgcaaagca gattacgtcg cccacaacat cggtgccgag aaagttgaac tcaggcggca
     3541 aggtgtcggc gcgaaggacc gaatcaccgg tgtcgaactg attcaaagtc gggctgacca
     3601 gatccttgaa gaggtagaca acccgatgcg tgccatcaaa atacacgccg tactgatcct
     3661 tgtccgtgat cgccgagctg cgggcgagcc gaagggtgga ggtcaggtcg cgattgacgc
     3721 tccgaaaccg gattcggtcc attgccccgt caaagcgcgg cacggccagg gaggcgacga
     3781 tgccgatgat aacaaccgtc accatcatct ccagcaacgt gatgccccgg cttgatgtgc
     3841 gcaggttttt cataagtaac ctctggctta tctgcggcgc cggaccgacc ggtcgccggt
     3901 gcttgctttc aacttcacgc gcgctgtgtg cagcatgagc cgtgccccgc cgccgggatt
     3961 tctctccggc cgcctcatcc tacgctctct cctgtccgat aacgccttag aggccatcct
     4021 cggacccggc ggcggcccgg cggaacattt cgcgcgccgc cgcccttaag aactgtggga
     4081 ccgctcccat actggtggga gcgggacaat ttcccgttga cggattacga gaacgggcta
     4141 tcttgaactc gtaccgacgt gttcagaccg agacaaatcc gccgtggttc atggcataaa
     4201 ctgcgctgaa aggaagtact gcatgtcacg ctctcggctc cgcgatctcc tcacctgtgc
     4261 cgttacaacc gccgccctgg tcgcggctgt cgcgttttcc gcctcggctg ccgaccgccc
     4321 ggccatgctg ctgaccgacc agccggtcgt tcaggcgaaa gcggcgccgg tcctgtccga
     4381 ccgcgcccgt gcctatctgg ccgggcaggc cgccgacacg gtcggcgtgt gggtgttttt
     4441 caccgacaag ggtatccgca cgaaagacga attcagcgcc cgcgccgcct cggtggcgat
     4501 gaccgacaag gtgctgcagc gccgggccaa agttgaccgc gaccgcgtcc tcttcgccga
     4561 cctgccggta gcggccggct acgtggacgc cgtggtggcg gccggcggcc accaccgccg
     4621 cacctcccgg tggctcaatg ccgccagctt cgacctcccc gcggccgatc tcgccgccgt
     4681 ggccgcgctt ccctttgtcg cggaaatccg cccggtcgcc gtcttcaagc gctctctgcc
     4741 cgacctctgg gaggttcgct ccgacgggcc ggggtcggcg gccctcgccc ccgacgcgct
     4801 caactacggc tacgcgcagg cccagcttga acaaatcaac gtgcccgccg tccacgagaa
     4861 gggactcgac ggctcgggcg tcacgctggc cgtctttgac accggcttcc gcaagaccca
     4921 cgaggtgttc gccaacgcgt tcgccgaggg ccgggtgctt gccgaatggg atttcgtttt
     4981 caacgacggc aacaccgatt atgagcccag tgtcgactgg tcctcctcca ccagccacgg
     5041 cactctcacc tggtcgacga gcggcggcta caagtccggc cggatcatcg gccccgccta
     5101 cagagccaac ttcctcctcg ccaagacgga agacgtccgc agcgaaacct cggtggaaga
     5161 ggacaactgg gtggccgcgc tggagtgggc cgacacgctg ggtgccgacg tcatcacctc
     5221 ctcgctcggg tactccgact ggtactctta cagcgacatg gacgggcaga cctgcatcac
     5281 gactatcgcc gccgccaccg cggccggtct gggcatcgtg gtcgtcaact ccatgggcaa
     5341 tgagggtccc ggctcgggga cgctcacggc gccggccgac gctcacccga tcatcgcctg
     5401 cggcgcggtc gacatcaacg gcagcatcgc cggcttttcc tcgcgcggtc ccacgtatga
     5461 cgggcgcatg aaacccgagg tggtggcctg cggcgtgtcg acctcggccg cgaccaacgg
     5521 ctccgacgtc agctacggga ccgccagcgg cacttcgcta tcgacgccgc tcgtggccgg
     5581 cgcggcggtc ctgctggtgc aggcccagcc ctcgttcacg cccgagatga tccgcctcgc
     5641 cctcatgcag accgccgacc gggcctccag tcccgacaac acctacggct ggggcctcat
     5701 cgatgccgat gccgcgacct catggggcgt gagcatggcg gccgattcca ccatcaacaa
     5761 cggcccgctc aaggtgcatt tcaccggcac ctccgctctg ggtccgacca gttggctgtg
     5821 gcgttttggc gacggcgact cggcgaccgt gcagaacccc gcgcacacct acagcaaccc
     5881 cggcgcctac gacgtctccc tcacggtgga gacgtcggag ttcggccaac tcacgaccag
     5941 ccgcccccac ttcatcatca tccttggcga cacggcccac tacggttccg actcgacctt
     6001 cgccgggagc gacctggtgg tttcgatcaa cgtgaccaac tcccaaccgc ttgcgcgcct
     6061 ggtcgtcccc ctcactttcg gcagcgccgc ctggctcact ttcgactcgg tcacgcgggg
     6121 cgaccgcacg gcctatttcg agcgtttgaa tccgctgaat tcgaacgatc tcacgcgcga
     6181 gtacgtgtac gaactcctcg ccgacttcgg cgggggctcg ccgccgctcg cgccggggtc
     6241 gggcgaggtg ctcaagctct ggttctcgac cgacgagctc gccctgggcg ggcagagcat
     6301 cctgatcgac agcatcgcct ccccctacgt catttcgttc gaggccggtt acctgaccta
     6361 ctccccgacg gtcgccgccg gcaccggcgc cctcatcgac gtcatgcggg gggatgtcaa
     6421 ctatgaccat gatctcagca ttgcggacgt cacgcacctg gtcgcccatt tctaccgggg
     6481 cgggccgccg ccggtctgcc ttcaggcggg ggatgtcaac cgcgacttca tgatgagcgt
     6541 ggcggacctc acctactatg tcgcctatct cttccggggc gggccgcccc cgccgacgcc
     6601 gtgacggcgc ctgcccgctc gctcgcaacc acaaatgccg cggccggtgt cttttcagac
     6661 accggccggt ttttttgcgg atgcgcctcc cgcgctagat cgtgaacttc ccattccggt
     6721 agatcacttt cccgtcggcc agaacctcgg cgtccttgcg catgtcgcag atcatgtccc
     6781 agtggagtgc gctgacgttt tttcctcccg cctcggcgaa ggcccggccg agagccatgt
     6841 ggagcgtccc gccgatcttc tcgtcgaaca ggatgtcttt ggagaagcgg tcgatgtcgt
     6901 agttggtgcc gatcgcgatc tccccgacat accgtgcccc cttgtccatg ttgagcatgc
     6961 ccgtgaggta cgcctggttc ttcgccgccg actctttcac caccttcccc ttcttcagct
     7021 ccaggcgcac gtcctcgacc tcgcgcccga cataaatggc cgggaaggag aaccggatga
     7081 cgccttcggc cgaatcttca atcgggcagg tgaacacctc gccgtccggg aaattctccg
     7141 tccccgcgca gttgatccat ttccgcccct tgacccgcat cttcaggtcg gtgtcggcgg
     7201 accggatatg caggcggtcc gtccggttga ggattttcac cagccggtcc tgctcctttt
     7261 tgactttggt ccagtgtttc accgggtcgc cgctggtgac gtggccggcc cggtagacga
     7321 agttttccca ctcggcgagc gacatgctcg cttcctgcgc ctcggcctgc gccgggaaga
     7381 gcgtgccgca ccagcgcagc gacttggtct ccatccgctt gaacagcttg tccgacagcg
     7441 gccgctggca cttccgctgc agcgcctgcc gtttgggatc gacccccgac aggtggcgcg
     7501 tgttggccgt cccccagatg tgcagaaaca catccatcgt gttcacctcg gtccgtgcga
     7561 tcggagagat gtacgcaaac tgggcgtcgg ttcccttctt caggaagatc tcgtcgtgct
     7621 gcgggataat gacccgcaca tagggaatgg ccccgatgcg cacggcctcc tcaaacacgg
     7681 ccgtcatcag cggcagcgtc acatactccc cctggatctt gagcagttcc cccttcttga
     7741 gccgcagcga atagtgcacg agcacctggg caagtttctc ggtccgcggg tccatacgtc
     7801 gctcctggtc aaagtggttg tatcgccagt accttcagtc tgggtcgcgc agggcaataa
     7861 cggcctccgc gtccggtttg taaagctccc gccgtcactt tccgggcccc gccgccggcg
     7921 ggccgcggct gatattcccc ttgaccgaat cctttccccg gttctatcgt aggagtgagc
     7981 ggagaccggg aatccggggc ccgcgcccgg cgacgaacgt gtgcgcaggc agtgcgccgg
     8041 ggcgccccag acaggaggtg tgtccatgaa cgaatccgcc gtctatgtct cgccgtcgac
     8101 cgcccgcagc ctctggcagg agtaccgcat ttacgaacgc catgtcgagt tcgacaccca
     8161 catcggcacc gtcaccatcc cgttcgacaa catcgagagc gtcgaggtgc aggggtcgga
     8221 tgtcaaggcg ctcctgagag gcgacctcag gctgaaaggg ttcaaaccgg cgatcaagct
     8281 cgactgggcg aatttcaccg aacacgtggt cctcgacaag aagaccggcg cgtgcaagcg
     8341 cttcctcttc accccgcagg acccgcacga attcaaggcc gtgctcgacc gcctgatggc
     8401 gaagcaccgc ggctgacacg gcggcggtct gtcattcatg gtgaggtcga ctgcgtgcgg
     8461 ggcgagctgt ctttcacatc gcgccacgga gttctcgccc aacaacgcat aattgcacgc
     8521 cgagactgac gcaatctccg cccgcgcgga tgatgtaacc tcacgtttgt cccccccctt
     8581 ttccgctccg cgacgaaatc cgcggctcgg gtggtaccca cccctccacc atttccttga
     8641 caaactgctt ctttatggta tgtatgaagg agccatgagg aggcttgatg cggtttctgc
     8701 tttggctatg catcgcggtg ttatcgtcgc cgtgtgcggg gttggccgcc ggcccagtcg
     8761 ttatcgatac cctaatctcc gataccgtcg ccagttggtt cgggatgagc attgttccta
     8821 tcggtgatca gaacaatgac ggctacgatg actttctcac attcgagttg ccgaggaagc
     8881 tctatctgtg cctcggcggc cccgtagaaa cagggatcac gatcacacgc attgacacgg
     8941 ctggatcccc gttaatcgga aacctgggtg acatcaatgg cgaccatatc accgattttg
     9001 ggtgccccat ccgactttcc agtatagaca gttatcgtct gggtgtttgg tacggcggct
     9061 cgccgctaga tgacggtcct gatcttgtat ttggggactc tacgtatttt ccaggggttt
     9121 cggccccggt gttaggaaac gacatcaatg ggaatggtac gcttgagttg atcagcagcc
     9181 actccgacaa ttgggactac ccgttgctga tgtttttcga attgggaggt cacgctgatt
     9241 cgatgcccga tctcttgttg agggcgccag gtactaacat ggccatggcc gatggcgtca
     9301 tcacgggtga ttttaacggt gacagcttcg acgatctagt tacgaacatg cgaagtatac
     9361 tcaacgactc cgttaagggg gaggtgctgc tgtactgggg gggaccgggc ttcgacacta
     9421 tccctgatgc tcggatcaca cggcgaggcg agtacagctg gctgtacgac acattcggca
     9481 cccgtttgga gtcactgacg gatgtcaacg gggacggctg ggatgacttc ttcattcact
     9541 acaggggcgg ctccggtgat tcgctctcgt ggattcactt cggcggcccc gccttcgaca
     9601 cggttccgga tgtgattctc accactagaa atcaaaaagc ccgccttgct ggtgatgtca
     9661 accacgacgg ttacgctgac cttatcacag ggtacgctac gtctttctcc ggcctcggct
     9721 acgccgacat ctacctcggt ggccctgact tcgacagcat ccctgatgtc gtcattgacc
     9781 actccatgtt ttccggctac tataactatt ttggacatga ggtggccggt atcggcgacg
     9841 tcaacggcga cgggattgat gattttgctg tagcttcatc ccgcggcagc ggctatccct
     9901 gggtcatctt catctttgcc ggctgggatg atgccgtcag cgtcgatgac accctcgacc
     9961 ccgaacccgc cctccccacc aacttcacct tagaccaaaa ctaccccaac cccttcaacc
    10021 cgatcactac catatccttt tcgctgcccc gcgccgagac tgtcaccttg cgcatcctta
    10081 acgcccttgg ccagacggtg gcggtgctga taaacgagca gcggctgggg ccgggggaac
    10141 atgcggtcgt gtgggaggcg gaggcgtatg cgagcggagt gtacctgtac gaactggagt
    10201 acccggccgg gaagcagagc cggaagatgg tgctgctgaa gtaagcactc gatcaggggg
    10261 ggccggtggc gcaccattca tggtgcggac ctcttagatt gggcctcgaa gccgtcagtg
    10321 tccagcaagg cactcggcca gccggcgcag gagcggcaac ttgatccaag gccagccctc
    10381 cggttgctcc gtcagaccgg cgttcacagg gtttgcctga gaggtgttga tcttctccct
    10441 cacttcctgc agcgcgcggt gattctggaa gatgaaacca ccatttttca tcaagcccta
    10501 attggtgtgc tgccggcccg agcatcagtg agcgggcgga tccaagcgaa agatcgaagt
    10561 ccgcgccggg tcaatcacat ttggtgagga agaggaaccc acgatgaatc gtggcgccgg
    10621 tgtgtcattt catacaccct caacgatatt accccagagg catgaaccag tgggtacccc
    10681 ttgacaaaac gtgtctcccg ctctataatg agttgtaatc cgaaaatgga agagggacgg
    10741 tgaaggtatt tccaataacc ggctcgcggg tcgtggtggg gctgttgctg ttggtgtcaa
    10801 tcggatcggc caatgacctc gcggagcctg tgtcgccgga cagccttagc ggtcgtgtct
    10861 acaatagcaa tccgacgccg tcgcgtacgc cctcggtgcc gttcgtcgat accgatacca
    10921 ccacgtaccc caagaaattc tggatggggc ataagcagca gctgtctcat cccgtccgca
    10981 gcatctaccc gcgcataacg gctcaggatt ccatcctctg tgtcacctac tcaagccggg
    11041 aggcggtcac gaaggccggg acgtggtggg tctaccgcgt gctctccttt gactatggct
    11101 tgacctggag tgagccgcgg gcaatcgaga ataccgggga ctttaacgcg ggcgacctat
    11161 ttccattgat tgagggcgac accattctgc tttctttttc ccgacaggtc gagggcatcg
    11221 agacgacgta ccagcaggtg gtggccaaag aatctcttaa ggatgccgct ctgacgaccc
    11281 cggttgttct gcagcagtcc actgcaaatc tccaccactc ccagttggtt agacaccagg
    11341 acacaacttg gctattctat gtattctcgg actgggacta caatcatctc gcctcctgtc
    11401 gctcaactga caatgggcag acgtgggata ccctgaattt ctccggcccc tcaatcggct
    11461 ggccgatgtc tttccaacgg gccaacgacc atatctcctg ggtaggagaa gagggggtgg
    11521 agagctactg ccgcctgctt aacccggacg gagtgacctg gactgatcgg gtcctgatct
    11581 cggagacccc ggccaacacg gccagccagt ggccgaacgc gtgcagcaac ggcctctccg
    11641 acctccacgc cggctggttt gacttcgacg gcgccgaggg gggctggtac ggctacatct
    11701 tctacaccca cagctacgac ggcggggaga gctgggggcc gatctggagt ctctcgccga
    11761 ctgacgactg cgagcactcg gccttctggg ccgactccca gcgggtgtac gcggtgtgga
    11821 acgactgccg ggaaaacggc tcgccggact tcgccctcta cctgcgctac tcgcacgatc
    11881 gcgggatgag ctggtcgccg gagatcgagg tagtggacaa gattgatccg gcgagagaac
    11941 cggatctcta cgggcagggt gatctggtgt acctcgtctg gaacgagcag catccgccgg
    12001 actggatctg ggggatctac ttccaggtgg gcgggtggta tatcccgggg gatgtgaacc
    12061 gctcggaaga tctcacggtg agcgacctga cctttttggt gagctacctc ttccggggcg
    12121 gggctgcccc ggtgctgttg ggggcggccg agatgaatgg cagtgcgccc attaacattg
    12181 ctgatctgac ctatttcgta gattatctgt ttcgaggggg cgctgaaccg gtcggcgctc
    12241 agtgacttgg ttcgcctgtt gctgtagagg aatagctttc ggcttgaaca tacacgcgat
    12301 tacgcggcgg gaggaacgag gagggaagac aagcggcagg ccacgagctc gcgggcggaa
    12361 taatccatag cgtcatcctg aggtagaata ccggttacgg cggcaaggca ggaccggcga
    12421 ggaaacgcag gcaaatggaa ttgcgggcgc taccgcagcg gatcggccgc cgcgagcacg
    12481 gagggcgcac cgtcaccgac cgcccggtcg agctcctcgc gaacggcccg gccgagcgac
    12541 tcgagcgagt acggtttgcg tatgtacgcc cccgccccca accgctgcat ctcctgcacg
    12601 cgctcggtgg aggagtagcc gctcacgatg accgttctct ggtcgggacg gcgcaggagg
    12661 atttcccggt aggtgtcgag gccgtcaaag tcgtcctcca tgatcatgtc gaggacgatc
    12721 aggtcggcga cgtgggtgtc gaggtagcgg agcgcctggc ggccgttttc ggccgccgcc
    12781 acacggtagc ccaggccggt gaggagttcg gaggccagat cgcgctgggc ggcagcgtcg
    12841 tcgaccacca ggatgttctc gccgcgcccg gagaagtcgc caaccggcgg gcgggccgcc
    12901 accggcgatt ggatggtgcg gaagtagacg acgaattcgg aaccccgctg cggctcggag
    12961 aacacgtcgt agtatccccc gtggtccttc accacgccgt acacgaccga gaggcccagc
    13021 ccggttcccc ccgcccccac catcttcttg atgtaatacg gttcgaagat cttgtccagg
    13081 tcggcggccg gaataccgcg cccggtgtcg cggacggaga gggtgacgta ctcgccggga
    13141 tcgatccggg tgtaaccgcc cggcagggtt tccaggcagc gccgcgcggt tgtcaccgtc
    13201 acctcgcccg ccgtgtcgat cgcctcgaac gcgttggcca gcaaattgcc caccagcttc
    13261 tgcaggtgcg gaaatgatcc gttcatggcg gtgaccgagt cgcagaggcg gagggtgacc
    13321 gccacctgcg gcttgcggga ggtgaggttg cggaaaggcg cggagtcgat gcaggcctgg
    13381 acgacctcgt tgagatcgac gggcgtcatg tcgtaccggc cgcgccgaga gagcgtgagc
    13441 aggtcctgga tgacctcgga ggcttccacg gccgatttgc gcatggattc gatgtatttc
    13501 cggacggggc tgtccggggg gagcttggcc aggatcagtt cggggtagcc gacgagcggg
    13561 ccgagcatgt tgttcagatc gtgggccatg ccgccggcga gaatgccgag ggcctccatg
    13621 cgctccgcct tttcgagccg ctcgtgcagg cgcgcctgct cctgttccgc gcgcttgcgc
    13681 tccgtgatgt cgcggacgat ggccagcgcg gaatcgggcg atcccatgag gaggcgggac
    13741 tcgaagtagc gggtgcggcc gccgatcggg agcgagtatt cgtacacctg cactgcgcct
    13801 gtggcggccg cctgccgcag gttctggata gtgatgtcgg ccacgtccgg cggcagcacc
    13861 tcgctcacat gcttgccgat gaactgctcg ggggcgcggt acatatcctc gcggctgcca
    13921 tggcacttct ccagaacgcc gccgcggctg agctggaaca cgagatcggg catggcgtcc
    13981 agcagcgtgt ggttctcccc ctcgcggcgg cggaggagtt cctcgatctg ccggcgttca
    14041 gccagctccc gccggtagca gaggttgcgg tagccgagtt cggagagctc gtgggcgacg
    14101 ttccacagga gatcgaggtg gcgttcaaac tggtcgaggg cgatcggtgc gacgcgctca
    14161 taggcggcgg cgagggcctc ctcatcgagg ccgatttccc tggcgaaagc cctgatgcgg
    14221 tcggagccta cccggtgggc atcgcactgg ccgatcagcc agtcggcgat gtggcggtcc
    14281 tccacgatga tcggggcggc ggcgtcaacg aagccgcaac tgaggcagcg gtggtgggtc
    14341 ggccccggct gacgacgggc gcggccaagg agctgctcgg aggacagggc gcagcgccgc
    14401 cgccccacgg gcgacgccag caccagccgg caaaccggcg aatagttgct cgggcgggtc
    14461 aggcagcggc cggaaaggtc aacgatgacc gaggcgacgc cggtcgcggc ggccagcgaa
    14521 tcctgaacgt gctgcagttc gtcgatcgga atgagatcga gcagggtgaa cgattcggcg
    14581 gcggggagtc ccgccaacgg ggtttcagtc atagccgggt tgctccggac ggcggcgctg
    14641 tgcactgccg tctgcatatg gttgcgggac cgtcggccgg tcctttcttt caaatgtcag
    14701 ccgtcagcac gccgggcatc acgtctcacg gaggcccaag tcgccgttcg gcgggagttc
    14761 ggccgggagc tcctcatacc gcaccgtccc cggaccgccg ccctcatcaa ggcggtgaca
    14821 tccttcaatg aggagggcgt cgatcgggag gtggctgttc ggttccggga ggctttcgcg
    14881 tccgaccggg tcgacgctcc agatcccgcg cgcccagccg agtccgaggt agacggcctc
    14941 ggccccgacc gtgtcaccgc actcggccca gatgagacgg ccgccgtcga ggtacatggt
    15001 caactgcgcg ccgcaggcgg tgaccgaaat ccgcacggtg cgctggctgt agccgagcgc
    15061 ctgcagcagg tcaatcaggt tcatatcggc gaggctaccg tgcgtcccca tcgactggag
    15121 catgctcatc tgtcgcgaat gctccgcctc gaggcgcaag cgcaggcgcc gcaaccgggc
    15181 ctcgcccaac tcggggccgg cctcgatcgg gagaatgtcc tcgatacggc tgtcaaggag
    15241 atcggccagg tcgcggataa ccctcgtcgg aacggcgagc agggcgggga cggtgtcgaa
    15301 agcgattccc cggtccgcga gatcgtccac catccggcgg gcgtcgcggg ccgacccgga
    15361 gctgacgatg acgagaaaat ccggccagcg gctgccgtac aggtcgatga accgggcggc
    15421 ggtgccggcg cggaggacct caaacccggc gccggcgagt gcgcccgcca aagcgccgac
    15481 atagccgtcg gcgtcgccca gcaccagggc ctgggtggag gcgcggtcga agggccgcgg
    15541 ggtcgggatc cgctcgctca ccatggcgat gaaagcgtcg accacctgcg ggaggaaagc
    15601 ctgtccgcgc aggggtcgga gctcggccgt gagcgcgcga tagagctcgg gggtgggatc
    15661 tttcagttgc ccccaccgat ccgcgagagt atcgaccagt cgaaggatac tgccgccgag
    15721 gacggccagc gggaggcggt cggcatacgt gtcggcgatc tcgctctccc ggtgcgcaag
    15781 gatcgccgcg acctcgggcg gccggcggtc gcttgggggt cgctcgggcg caccggcggc
    15841 gatcgccgcg tgaaccacga ggagccggtc gcgcggcgaa agcttgaggc ggtcgcaaag
    15901 ctgtgcggtc aggtccaccc tctcggccac tcggcgtacc ttcagttcgt cggccggggc
    15961 ggcgcccgcc ggccacatgg ccagcgcggc ggtgaaaagg ctgccggtca attccgcagg
    16021 cggagccgcg gtgatcaggt cggcgacccc ctcggcaaag caaaccggac actgaggagc
    16081 cgtggcacgg aggtggtcct cgaggacggc gtgagtttcg ttcggccggc tccggacgac
    16141 gacagcccgc ggcgccgcag cggcaatttc cgccagcgct tcatcgaccg tctcgacaag
    16201 gatgacgctc tggccctcga gggcaagcgc ccgggcgagg gtctcgacag ccgggtcgat
    16261 cgcgccgaca aggaggatcg gccgcctcgg cgccggggga gcatcggggc ccgccgcgcc
    16321 ccgtgtatcg tccacctccg gccggttgcg ataagcctca atgatgcggc agcggaggac
    16381 cggctccagc gcggctgcga ggtgaatctc ctgcacgccg gtgaccgccc gaatctcggc
    16441 cgccagacct tcatcgcggg gatcaagaca agcgaccgtc aaccggttcg ccgccggatc
    16501 gaacgccacc ggcagcaggc aacgctgctc ggcgaaccgg cggggaagat agccgatcac
    16561 ggattcgggt atggggaggc cggaaagcgc gatcgcgtcg catccggccc cgctgcggat
    16621 ggcgccgacc aactcctcct ccgtcaacgc cttgaggcgc aacaggtggc cctcgaaagt
    16681 gccgccgagg aggttttggc gacggtgcgc ctcctcggcc gaggcggccg agaccaggct
    16741 tgtctccacg atgagattgc ttaccgacac gagatccctc tttcgagtcg ggaggtatgc
    16801 gccgcgccgc tggaagcggc gcaccggcat agtcctgcct ctatacccat aatggtcgga
    16861 caatcccagt ggaagttgag cgggggaata acagggggca aaagaggaca tgggtagtcc
    16921 tgttgccgcg caccggtgga cgggaggttg ccgcgagtcg gggcgcgctc cgcttgcgaa
    16981 cgaggcaaca ggtgcttgcc ccgccccggc tttctgctta tcatgttgtc cccgtcggcg
    17041 ggccggtcga ggccggaggg cagccggggc gacaaaggat aatattactc gagacataag
    17101 gagacgctgt catgtaccgc attccccagc cgaaaaatga accgattctg aactatgctc
    17161 ccggatcgcc ggagcgggcg gcgctgaagg aggcgatcaa gttcttgaaa gccaacccga
    17221 tcgacgtgcc gctcgtgatc ggcggacagg agatcaagac cgatcataag atcaagatca
    17281 cggcccccca cgaccacaat ctcctcttgg gacattacta ccagggcggc gagaaagagg
    17341 tcaagatggc ggtggaggcg gcggtcgagg cgcagaagag ctggcagcac atgccctggg
    17401 agcagcgctc ggcgattttc ctcagagctg ccgacctcat ctccaccaag taccggtaca
    17461 tcatgaatgc ggcggcgatg ctggcgcatt ccaaaaccgc gcaccaggcc gaaatcgacg
    17521 cggtggcgga actggccgat ttctaccgct tcaactgctg gtgcgcccag cagatctacg
    17581 acacattcca gccggagagt tcgccgggcg tctggaaccg gatcgatcac cggccgctcg
    17641 agggtttcgt gttcgcggtg acgcctttca atttcatctc gatcaacggt aacctcccgg
    17701 tcgccccggc gatcgtgggg ggcgtctcgg tgtggaagcc ggcctcgacg gcgacttaca
    17761 cctcgcactg gattatgaaa atcctccgcg aggcggggtt gcccgacggc gtggtgaacc
    17821 tgctgtttgc gaaggggcgg gacatcggcg aactcgtgct caagagccgc cacctcgccg
    17881 ggatccattt caccggctcg acgccggtgt tccagatgat gtggaagacg gtcggcgaca
    17941 accttccgaa ctacaacacc tacccgcgcc tggtcggcga aacgggcggc aaggatttca
    18001 tcatcgcgca tccctcggcc gacgtcgacg cggtggtggc caacacgatt cgcggcgcct
    18061 tcgagttcca ggggcagaag tgctcggccg ccagccgctg ctatttcccc gaatcgctct
    18121 ggccgacgat caagcagaag atgatcgccg aactcgggac gatcaagatg ggggatgtcg
    18181 agaacttcgg caacttcatg ggggccgtga tcgacggcgc ggcgttcaag tcgatcaccg
    18241 agtacatcga ctacgcgaag cagcataagg acaagtgtga gatcgtcttc ggcggccgct
    18301 acgacgattc caaggggtgg tttatcgagc ccactgtggt ggccacaacc gacccgcact
    18361 tccgcctcat ggtcgaagaa atcttcgggc cggtgctcac gatctacgta tacaaggacg
    18421 ccgagtacgc caacacgctg cacctgtgcg accagacctc gccctacagc ctcacgggcg
    18481 cggtgttcgc gcgcgaccgg atggcggtgg cgctggccga acggacactg cgccacgccg
    18541 cgggcaattt ctacatcaac gacaaaccga ccggcgcggt ggtggggcag caaccgttcg
    18601 gcggcgggcg cgctagcggg accaacgaca aagccggggc gatccagaat atgatgcggt
    18661 ggatctcgcc gcgcacggtg aaggagaatt tcattccgcc gaccgactac cgctacccct
    18721 tcctgaagga gtagcgggag cgggacgcgg agatcgtgcc ggctgccgca ggggccgccc
    18781 gtcatcgccg ttcgggggcg gcgctcggcg gggtctcccg gctcccgtgg agcctctgac
    18841 atcgacccgc ggcgggagcg tcgatgcggt aaagggaact cggtccgcct ggaccgtggg
    18901 ggcgcctttg ccgagatttc tgctgcttcg gcaccggaac gaaagatatc ccggtctgcg
    18961 gcaggaccgt tatcctatgg caggggcggc agggttgcgg gcggctcgga attgcgccgg
    19021 cgcggagtcg gcggtaacgt cccccgccgt tgccgcggcg catgtctttc ctccccaaag
    19081 tataagccga ataggctgtt actccgacgt acatcaccgg agccacggtc gataatcgct
    19141 tctgcgatct gcctcggatt caattttttc cactgcgccc gagttcctgt gtctgctgct
    19201 gcatcaccca gttgcgcacc cgctgaacgt cgaccggctc gccgcgtacc tgaaaaccga
    19261 cgagataccc ggcggcggtc ttgtttgcgc gcagccgcct tccgatcgtc gaagactgca
    19321 caacgggcgc cggcaggcag gggtagtacc ctggcgcaat ctggacaagg gtcacaatgt
    19381 ccgcgaaatt ctcgcgcagc tgcttgacaa attccttcac gtcgccctga gtcgcatctc
    19441 tcgcaaattg catccatcgg acgttcgctt cgagtgtgcg cacatcacca agctccagcc
    19501 agtttgactg ccgacggctc gtaatgctga agccggtgtc cgtacgttcg acccagtacg
    19561 aggcggtatc cgatcggagt gtgacgtata acacaaatga ctctgccggc agagaaaccc
    19621 ggtcttcccg ctttccgcga ttgagcgtgc gcagacgcag cgtgagcgac gaatcggtgg
    19681 tgatggcatc aaacccaact gttcggtatg cggtacggat gtcgattccc atccgaccgg
    19741 tgtttctcag cacctcatga tccggctcac attccatgta gacatacaac tgaaaacgct
    19801 cgatgagaag caccggctca ggcagaggtt gttcgtctga cataaccgtg tcctggtcca
    19861 cgtcccattt aacatcgtcc atgccggccg acccaccaga gcagaccagc gacagactca
    19921 acaggatcag ccgcatcaaa agtttcatga cggctccgtt catctcctcg catatcaatt
    19981 ggccgaattc gcttcctcta caacgacgcg acctcccacc aagcgggcaa tgtcgcggga
    20041 taggtggtgc tgccgcggcc tcagccgcgt tgtctgcatg ggttctgaca tcaacttgcg
    20101 tgccggaccg cgagcttacc gacatctttc tgggcatcgc attgacgggg aagcagttgg
    20161 cgaggaagac ggagagcgcg cacgagcagc cgcatcgggg tttgcgcgcc gttgtgacgc
    20221 ccacggtggc gcagcgtgac gcatggtggg cgaagccgtt gggagtaaag gcattaataa
    20281 ggcagtatct gcgtcgggag tgcgtcgggc gactcgcccg tgacgcaggc agggggatct
    20341 gcgatggctg atcgctgcag gaatcagacc ggcgccggga atctcgtgca tgcacgtcga
    20401 attccgcact ccggcgttgg acggccgacc tccctcagtc gccggcagac ccaggggcgc
    20461 gctttaggct tgtgcgccgg cggatggatg ctacattggg ggcgattcag gacgcccgaa
    20521 agaacggaat gaccccatgc aactcgtgct ggccaccaac aaccgcgaca aaatacggga
    20581 aatccgacac cttcttgagg atctcccgat caccatcctc acggccggtg attttctcga
    20641 ttttccggaa atcgacgaaa ccggggagac gctcgaggcc aacgccctgc tgaaagcgcg
    20701 ggggatcgcc gctttcacgg accttccggc cctggcggac gattcggggc tggaagtgga
    20761 tgcgctcggc gggcagccgg gtgtgtactc ctcgcggtac gccggggaca acgtcaccta
    20821 cgacgacaat aaccggaagc tgctggagga gttggcgggg gtgccgcgag cggagcggac
    20881 ggcgcggttt cgctgcgtga ttgcgatcga ctgggccgac gggacgcacg agacggtcga
    20941 gggggtggcc gaggggtgga ttgccgagga tatcgcgccg ggaaacgggt tcggatacga
    21001 cccggtgttt gtgcacagcg aaaccggaca gcggttttcg gccatgaggc tgccggagaa
    21061 gagcgcggtg tcgcaccggg gccaggcgct gcggcgggcg cgcgaggcaa tcagcgcgcg
    21121 gctgaaagcg aggagctgag gggaaacccg ggcggccgcg gggcgccgcc ccgggggacc
    21181 gacggcagcg cagtcggccg ccggcacggg cgggacgctg aaattgccat gcgcgcgggc
    21241 agcggagagc gtatattccg caagaccgcg ccggcagacg accaacggtg accgcagtga
    21301 agaagaaaac gcgcacacca tcgcagctcg ccctcctgat cctggtctgt ttctttctct
    21361 ccggattcac cgggctcatc tacgaggtgc tgtggacgcg gatgatcgtg aagatcatcg
    21421 gcggagcacc cttcgcgatc tccatcatcc tcacgatttt catgggcggg ctcggcttgg
    21481 ggagctacct ggcgggccgg gtggtcgacc gggtgaagca gccggaagaa ctcgtgcggc
    21541 tgtacggata cctggagttg atggtggggg cgtacgggct ggtgctgccg gggctgatgg
    21601 cgctctttcg accgctgttc gcggccatgt acaacggcgt gttcgaatac gggatgatct
    21661 acaacctgct cacgttcgcg ggctgcgctc tcctgctcat tctcccggtg accttcatgg
    21721 gagcgacgct gccggtgttg agccggttct atgtctcccg cctggggcat ctggggacgc
    21781 acacggggcg gctgtacggt ttgaatacga tcggggcggg attcggttcg ctcgccaccg
    21841 ggttctggct gatcgcgtgg ttcgggatgt ggggaacgct ggcgacggca gtggcgctga
    21901 atgcggcgat cgggctgacc tgtgtgctgg tcggggggcg cgcccggatc acccggggcc
    21961 ggccggcgga ggcgggagcg acgggcccgg cggaagtcgc ggagacgccg ccgcgggcag
    22021 tcatcagcgg cgcgctcgtg attttcgccg tgtccggctt ctgctcgatg tcgtacgagg
    22081 tgatctggac aaaactcctc gggctgattg tggggccgac cacctactcg ttcacagtgg
    22141 tgctggtgac gttcattatc gggctggcgc tgggagcgat gctgttcggg tggctggccg
    22201 atcggacgcg gaaaccggcc ccgctgcttc tggtgacgca ggtggcggcg gcggccgcgg
    22261 ccctgggcgt gagccaggtg ctggggaaca gccagttgtt cttcaccaag ctgattgcga
    22321 cgttccagga caatttcacg gcgctcaacg cggccaaggc ggcgctgctt ttcctgttca
    22381 tgctgccgcc gaccctctgt ctcggcgcca ccttccccct ggtcgggaaa atcttcacgc
    22441 cctcaacggc gcgggtgggg cggtcgatcg ggtttgccta cgcggtgaac accatcggag
    22501 cggtcctcgg gtcgttcctc gcggggttcg tgatcgtgcc ggcactgggg aaagcgaatg
    22561 ggctgagcct ggtgatcgcg attcagcttg ccggggcgct ggttttcgga tgggtgctcc
    22621 tgcgggcggg ggcggtgcgc cgacgcatct ggctgccggc ggcgggcgcg gcggcggccg
    22681 cgctggtcct cgtgttcgcc tacccgcgct gggaccaccg gctcctggcc ctgggcaagt
    22741 accaccggat cgcggaccag ggagtgaagg ccgaccaggt ggggtggtgg tcggcgctgc
    22801 tgcgcggacc ggagatcctc gcgccgctgt tcaatgccga cgtcgtgtac tacggggacg
    22861 gggtggccgg gaccaccacc gtgctcaaga cgcgcgacgc catggggaac gaggatttgt
    22921 ccatgaccaa cgcgggcaag cccgaggctt ccacccgcgg cgacatgccc acccagacgc
    22981 tcctggccca tttccccctg ctgttcggca acgaggcgaa gcaggtgatg gtcgtgggtc
    23041 tcgcgagcgg tgtgaccgcg ggggagattc tccgctaccc gatcgagcgg ctcgacatcg
    23101 tcgagatcag ccgggaagtg gtgacggcga gcgagtattt ccggccgtgg aaccacggcg
    23161 tgctcgatga cccgcgggcc cggctcattg tgcaggacgg ccgggcgcac ctggccatga
    23221 ccgcggcgga gtacgacgtc gtgatttcag aaccgtcgaa tccctggatg gccgggctgg
    23281 cgacgctgtt cacgcgggac tatttcacct atgcccggga ggcgctcacg gaggggggga
    23341 tgttcgtcca gttcatccac acgtaccaga tggactggcc gacgttcgcg atggtggggc
    23401 ggacgttcgc cgacgttttc ccgaacgcgc tcctggtggc gacctcgccg gacacggcca
    23461 gcaatgatct gatgcttgtc gggatcaagg ggagcgggcc gctgaatctt gcccgcgcgc
    23521 gggagatggg acagtactgc cggcagggcg gcaacttcgc ggtccgcgat cccgccgtgc
    23581 tcgtccggct ggtccgggcg cagaacctga aagagttgtt cggcggcggc ccgctaaaca
    23641 ccgacagcca cccgcgcctg gagtatgcgg cgcccaagct gctgtaccgg tcggaccccg
    23701 aaatcgcggc ccggatcgcc gccgggggga gactgacgga gccgctcgcg cggctgaagg
    23761 agaaagtggc gaccgaggtc gagtcgcaga tcgattttct ggaatacgaa ttgtcggtac
    23821 atctgccgat gcacggtttt gtgccgccca ttctcgcata cgaccgggcg accccagcgc
    23881 agcgggcgcg gcacgacagc ctcctgtgcg catactgccg gcaaaacccg gtggtgctct
    23941 cgctctgcca ggacgagcgg gcgcgggaat cgtgcgccgc ctgccagatc gaggggcttg
    24001 aggcgaccgt ggcggccggc cgggcgaccg ccatgaccta cagctacctg gcttttctct
    24061 acagccgggg cggccagacg gacaaactgc ccgcgctgta ccgggagtgg gtgcagagcg
    24121 atcctcgcga ggcgaaggcc taccacggtc tggccgcggc gctgatcgaa cgcaacaacc
    24181 tgaccgaggc cttgcccgcg ctgctgacgg cggatcggga gaatccgggc aatccggtca
    24241 ttctgaccga tttgggctac ctctacggcc ggatgggccg gcccgacctg gcgcgggact
    24301 atttcggccg agcgctgcag gcggacatcc ggtacgcccg ggcgcacatg gggatggggg
    24361 cggccgaggc gaccgcgggc aacatcccgg ctgctatcgc acactaccgc gaggcccttc
    24421 gcctcgaccc ggccctggag ggggctcaga cagcgctggc caagctccag gcgggccggc
    24481 agccctgacc cgcggcccag cccgcaccac ccgtttcaac accgcaagcg cggaacgaac
    24541 taggcctcgc gcgccgcagc ctgaagttcg gcgagaaaga gctccgggtc gacgccgtgc
    24601 atgcggcagg cgaggtcgat cgactcatac gattggccgg ggcagtcgag acaaccgtcg
    24661 ccgaaatagc gctggaagat cacgttcgtg cgggggtggc gctcgagaac ggcgccgatc
    24721 agggtctcgg aagtgatgtc aaacggggcg gccgaggagg cggcgctacg ggcgggggac
    24781 ggcggacggt tgacctcgtc ctcggcggcg cggatgagcg tctggaggtc aaggccgtgg
    24841 ttggcggccg ccatgccgag cgtgacaccg actttgcgga ccttgtcgag gtgccccggg
    24901 acatcgagca ttttcaaacc ctgcccgacg aggacctcgc gcaccgacgg gcagcgctca
    24961 atgaggtcgg cgatccgcgt gtcttcggtc aggctgatcg acggggagag gggagccgat
    25021 ggagcagcgg cggcgcctgc gggggccttc ttgagggaca gggtgaccca gatgttgacg
    25081 atgaacatga gcaaagagac ggcctggacg gcggcggcgg cgaggaagag aacgtgccag
    25141 agattgctcc cgtcgcggga gtagaggccg cgaaagagga ccatcccaac caggctgacg
    25201 ttgccgagcc agaagtggac gggaacccag gcgggccagc gcagctcggc gccgttgaaa
    25261 cggggaagta tgaaatagcc gatgccgaag acgatcatgg ccatgaagcc aagcaggttg
    25321 aagtgggtgt gggcgaagac ggcggcgtag cccggagatc cgccggcgct cagaatgccg
    25381 cacagggcgc ccgccgcgag atagagcagg gacatgtaga cgaagcgttt catgtaggtc
    25441 gacatggcag tctccgaatt atggttttcg ttggcgcaat gtcggcctcg gggccgggga
    25501 gagccttgac ccaggtcaag aagccggggg gaaagagccg tcatcggggc tgcccctcga
    25561 ctccagccgg gaatggtgtt gcggcaaggc cgcgggtacc gtattatggc cgccggagcg
    25621 cgcggccggg aaaggcagga taggatagtg ggatcagaga caggaaagat aggaaagaca
    25681 gttggcgccg ggaacgcccg ggtcgctacc gggttgactg tcgctctcgc gatcgcggtc
    25741 gcggcggtca ttctgccgaa gctcgcgctg agcggggcgg tcgcgcggat tctcaccacg
    25801 caggcgctcg aactggggct ttcgctcggg gcgatcgccg ttttcgggcg cgggcgattc
    25861 gccaactacg ggtttcgtct tccccgcccg gatcggcact ggtcggccgc gctagccggg
    25921 tggatgccga tcggcctgac cgcgctgggt ctgggaacgc tcgccacggc ggcgatgctg
    25981 gcatgcgggg ggaaaggaaa cccgctggtg cgagagctga cttttccgca ggtgatcctg
    26041 ttcgtgtgga ttttctccag cacgatagaa gaggttttca cgcgcgggtt cttgcagagc
    26101 catatcgcct ccgccgggga gccccggctc gggccgaggt ttctccggat cgggcgggcg
    26161 gcgctgatca gcgcggcgtt tttcgcggcc atgcatctcg tgctcctgct cagccacacg
    26221 gatgccgtca cggtcgtaat cattctcctg ttcaccttct ccgtgggatt gctggcggcg
    26281 caccaacggg agcggaccgg ctccctggtc ccggcgatcg gcgtgcacat gctggcgaat
    26341 gtcgggggcg tgttcggggg gatcgtgtac gcgatcatcg cgatgctgag cggcggagcg
    26401 gttcccggcc ggtgaggaag ggcgggcccg accgctcgct ctctctgccg gctagaattt
    26461 cgcccagcgg gggacgcggg ggaaggggag gacgtcgcgg atgttctgca tgccggtgat
    26521 gtacatgaca gcgcggtcga aaccgagccc gaacccggcg tgcggcatgc tcccgtagcg
    26581 gcgaatgtcg aaataccagg cgtagttctc ccagttggtg aggcccttca tctccatgtg
    26641 cttctgcagc acgtcgaggc gctcctcgcg ctgggagccg ccgatgattt cgccgacgcg
    26701 ggggacgagg acgtccatgg cgcggacggt tttccagtcg tcgttcaccc gcatgtagaa
    26761 ggccttgatc gcgagcgggt aatcgtacac gatcaccggc cgcttgtaaa ccacctcggt
    26821 caggcagcgc tcgtgctcgg tctggaggtc cagcccccac tcggcgggga actcgaactt
    26881 ccggccggat ttctgaagct gcgcaatcgc atcggtgtag ctcacgcgga cgaactcgga
    26941 ggcgacgatg ctctcgaggg tctcgatcag tttgtggtcg atccgctggt tgaagaaggc
    27001 catgtcgccg gcgcagtcgt cgagggcgaa gcggaagagg tatttgagga agtcctcggc
    27061 gaggtccatg tcgtcctcga gctcggccca cgccatctcc ggctccacca tccagaactc
    27121 gctcgcgtgg cgggcggtgt tggagttctc ggcgcgaaaa gtggggccga aagtgtagat
    27181 gtcgccgaga gcggccgcca ggagctcccc ctcgagctgg ccggaaacgc tcaggtaggc
    27241 ctcggcggcg aaaaagtccg cctcccagtc gacgcggccg tccttcttcg gaggctggtc
    27301 gagggggagg gtggtgacgc ggaagagttc ccccgcccct tcgcagtcgg aggcggtgat
    27361 gatcggcgtg tggatatagt agaagccgcg ctcctggtag taggtgtgga tggcgtaggc
    27421 gagccgggag cggatgcggt tgaccgcgcc gaaggtgttg gtccggggac gcaggtgggc
    27481 catgtcgcgg agggtctcga aactgtggcg cttcttctgg atcgggtagg tggcatccgc
    27541 ttccccgacc aggtcgagcg cctcgaccgc gacctcgtac ttctgcccct gggcgggcga
    27601 ttcgatcagc ttcccgcgca ggcggacggc ggccccggtg aggatccggt cgatggcagg
    27661 aatccggtcg gggtcggaga cgacgccctg gaggttctgc atgcaggagc cgtcgttgac
    27721 ctcgacaaag gcgacttcct tgcttcggcg gatggtgcgc acgaggccga ggatggtcac
    27781 ggtggtccca accggcacct gttcgtcgac gacaattcgc ctgattctgg tccggacttt
    27841 gtaatccatc ggtgtcttct ccgcgtcagt tccggtcacg gggcacatcg ggctgtcggt
    27901 ggcaataaaa cgagcggggg gcggtttggc aagtctcaag aaacgggcgg ccgggcgcga
    27961 aatcccgccc gcccaatgaa acttccgccg cgctcgcagg tattaaaggt gagaatgaac
    28021 tgagtgtgac tccttcgaga gacgttccca acttctacat agaaacccgg cctggctgac
    28081 aggccgggtt tttatttgcg ccggatcgcg cgccaacccg cccggctttc tcgcccagcc
    28141 ggcggggggt gcttgacggc gggcggcggg aaaccgagat tgcgccatgt ataaacgtca
    28201 gtctgtcgat ccggccgagg ccggggaggt cgtaatatgt ccacacaaga ttcatcgccg
    28261 cggtccgatt ccggggccga ctctgccgcc gggcacggat ccccgctcgc cgcggaagtg
    28321 ctgcgcctgc tgtacgaacg ggcgagttgc cggaatttcg ctcaacggga gatccccgac
    28381 cgcctgctcg acgaaatcct gcaggccggg gtgcatgcgg ccaccggcgg aaacctgcag
    28441 ccctggtcga tcatcgtggt ccgggatccg gaggcgcggc gaaagctggc ggagctcaat
    28501 gagaaccaga cgtttatgca ggatgccccg gtgaacctgg tgttctgcat cgactggcga
    28561 cggctggagc ggtgggcggc gggggcggcg gcgcccttca ccgccaccgc ggccttccgg
    28621 catttctgga tttcctttca ggatacgatc atcgccgcgc agagcatcgg caccgcggcc
    28681 gacgcggtcg gactcggctc agtctatgtc ggcacggtgc tcgagtgctt cccggaggtt
    28741 cgggagatgc tcgcgctgcc cccgggagtc ttcccggtgg tcattctgac gctcggctgg
    28801 ccggtccacc ggccggctcc gcgggggaag ctgcccctcc aggtcgtggt gcaccgcgag
    28861 caataccgcg agctgcccga tgaccaactg gcggcggcgt acgagacgaa gtacggcggc
    28921 gcgaagatcg actgcacccc ggaccgcctc gaccggctcg tcgaagtctg ccggagaacc
    28981 gggggagagg agctggcgga gcggtgccgg gcgcgggtcc gggcggcggg atccatcaac
    29041 atggcccagc ggtatttcgg cctgcactac ccggcggatt tcatgccggc ccaaaacgcc
    29101 tcctacctcc ggatcttcga agacgcgggg tttgaatggt tccgggagtg gtggccgcgg
    29161 gagtgagcgg atccgcgcgc gcggcggcga ggcgccgcaa aaaaaacctt ggagaatcgg
    29221 acaagaagca ccatattgaa agcatcaggg tctttcccga tcgcgcgttc tcgcaatcga
    29281 tctacgaccc cggtttcctt ccgcatccgc cggccgaggg atccgggcgg acagccgaaa
    29341 gtgacgggcc ggcggccggc cacagacgag aagactgaat cagcgtattc gcattccaag
    29401 gaggcttcca tgtcatctcg cgcagcggtg cagcgggtcg cggtcgcgct ggcggcggtt
    29461 ttgacggcgg tgctcggagc agcccagccc ggctgggggt tgccgaacgc gccggtcaat
    29521 cccggcagcc cgcccatgag cgaccagaac aactcggagg tgtctctcag tgagacgacg
    29581 ccgggcgaag tgtacgccgt gtggacggag tacacgccgg ccgggttcgg ggtttcgaac
    29641 atcgggtggg ggttttccaa cacgctcggg gcggcctggg tgcaggcgct gattcccccg
    29701 acgccgccgt atggcgcgga gtggaatccc tcgctggcct cccatccggc gggcgccttc
    29761 tacgcggcgg cggcggccta cggtcccggc atgccctggc tggcgccgga tgcggtggtt
    29821 gtgcacacga gcgcgggggg cggggcggcc ttcgggccgg gcgtgcccgt agccgtcaat
    29881 gtcccgggta ccacctggct tgattatccc gatatcgccc tggccgacaa cccggccatc
    29941 ccggcacccc tgttcggcac gatgcacacc gcgtgggtgg agtacgccga tgggacgttc
    30001 ggcgatgccg atgggaacgg caacccgttc gatgacgccg gggacaacta cacgatcttc
    30061 tatgcgtatt cgcggacgat ggccggtccc gccccgatct tcccggcctt ctcggttccc
    30121 gtggcgcttt tcccaggacc ggtcttcccc aatgcaccgg cggccaaccg gccgagtgtc
    30181 gcggtaatgg ccccgccggg caacccggtg attcccgggg gtggggtgta tgtcggctgg
    30241 accgacgggc tgaatgtcta tgtgaccggt tcgccggcgc tcggcgcggc cttctttgcg
    30301 ccggcgttgg tagcggcgat cacgcccgtg ccgccggtgc tgatcggtgg cgtcaagggg
    30361 gcggccaccg tcaccatcgc caccggggcc ggaccctgcg ccgggtgggt gtacgccgcc
    30421 tggacatcgg tgggcggagc cgacctggac atcttcttct cgtccagccc gaccggactc
    30481 ccgggctcgt ggggcccgcc cgtgcgggtg aaccaggatc cgatcggcaa cggcgcggac
    30541 cagtgggcgc cccacatgtc ggtcgacccc ggctcaggcc gaatcctcat cacctactat
    30601 gaccgacggg tcgatccggc caacgtggcg caccaaacct gggtgtcgtc ctccccggac
    30661 tgcggggtga cctggctcga ttgccagttg tcgacgacgc cgccggccgc gccgatttcg
    30721 accttcccgc tgccgcccgc gccgatctac ctgggcgact acctcggctc cgacttcaac
    30781 ctgctcaacg gcccggcgca catctggaac gacggccggg cgccggccgc ggttcaggac
    30841 gtctatttcg aaaatgtcat gacttgtttc ccggataccg acggcgacgg cgtttttgac
    30901 ccgtacgaca actgcccgac tgtacccaat cccggccagg cggatgggga cggcgacggc
    30961 gtcggcgacg tgtgcgacaa ctgcccggct atcgctaatc ccggccagag tgacggcgac
    31021 ggcgacacct tcggcgacgt gtgcgataac tgcccgacgg ttgcgaaccc gactcagttg
    31081 gacggggaca gcgacgggat cggcgacctc tgcgacaact gccccggcac gcctaatcca
    31141 gggcagctgg acggggacgg agacggagtc ggcgacgtat gcgacaactg ccccggcgtg
    31201 gccaacccgg gccaggccga caacgacacc gacgggctcg gggacgcctg cgatccggac
    31261 gacgacaacg acggcgtcct ggatccatcg gacaactgcc cgctgatctc gaacccgggg
    31321 cagtcggata tcgacgccga cggcatcgga gacgtgtgcg atgccgacgc cgacggcgac
    31381 ggtctgctga acggggatga gttgacgatc gggagcaacc cgctgtccgc cgacagcgat
    31441 gcggacaatg tcaacgattc ggtcgaggtc taccactgcg gcggggggat cgggagcccc
    31501 tgcgacacgg acggggacgc gactcccgac gtgatggatc ccgacgacga cggcgacggg
    31561 atcaacacga tcaacgagga tacggacctt gacggcaatc cgcgcaacga cgacatcgat
    31621 tccgacgggg tccccaacta cctcgatccg gactcggacg gcgacggcat cggggatatc
    31681 agcgacaact gcccctacgt gtcaaatccc ggccaggtgg atacggacac cgacggtctg
    31741 gggaacgcct gcgatcccga tgacgacaac gacgggatcc tcgacggcgc ggataactgc
    31801 ccgctggtgg ccaatccggg gcaggaagac gccgacgggg acgggatcgg tgatgcgtgc
    31861 gacgggtgct gcacgctgcg cggcgatttc aacgacgacg gagcggtgaa agtgtccgat
    31921 ctgacggccc tgatcaacta cctcttccgc gggggcgcgg cgccgtcgtg cctcgatcat
    31981 ggagacacca acgcggacgg gtcgatcaag gtgtccgacc tgacgctcct gatcaattac
    32041 ctgttccggg gcggtccacc gccggcggct tgctgacgag aggcctgttc cgcgcacagt
    32101 gagatggggc ccatggcgac cgccgccatg ggccctcgct tttgggcgtc gcggcagccg
    32161 gaggcgggca aaaaaatgag ggtggcaggg attgaacctg cgacaccctg cttaaaaggc
    32221 aggtgctcta ccactgagct acacccccaa agggacaccc atcgccggtg gcggcgggtg
    32281 tgcggtcaat aatagcaggt cggcgggccg aggtcaaaga gtttttggcc tctccgcgcc
    32341 cgggcgcgcg gcctagccca cggcgatcgt ttcatcgcgc cgggcccccg tggagacgat
    32401 ggcgatggtc accccgagat cgcgggcgat gaagttcagg taattgcggg cctgcgccgg
    32461 gaggtggtcg aacaccgtcg tccccgtcgt gcgcgtccgc cagcccggca acgtccggta
    32521 ttccggcttc acccgggcga ggtcggcaag gtcgagcggg acctggtcaa actgctcgtc
    32581 gcccagacgg tagccggtgc acacggggat ctcgtcaaaa tcatccagga catcgagctt
    32641 ggtgatggcg atcgaggtga cgccgttgat gcgaacggcg tggcggagct ggacgaggtc
    32701 gagccatccg cagcggcggg ggcggccggt cgtggcgccg tattcatcgc cgcgggtgcg
    32761 aagcagatcg ccggcgttgt cgaccagctc agtcgggaac ggcccggcgc ccacgcgggt
    32821 cgtgtacgcc ttgaccaccc cgatcacctc gtcgatcatg tgcggaccga tccccagacc
    32881 ggtgagcgcg ccccccaccg tggtgttgga cgacgtggcg aaggggtagg tcccgagatc
    32941 aacgtcgagc atcgccccct gggccccctc gtagaggatc ttgcggccgg cgcggtacgc
    33001 ccggtgcagg gccagcgaga catcggtcac cagcggccgg aggatcgggg cgatttccat
    33061 gagcgcatca aaagtgcggc cgaggtcggc gcgctcgtcg gacgaaccct cgaggtactg
    33121 gcgcttgacc gtcgcctgat actcgagccg cttgcggagc cggtccggat ggaagaggtc
    33181 gatgacgcgg atgccgttgc gggcgacttt gtcgatatag gcggggccga tcccccgcat
    33241 ggtcgtgccg atcgaggcgg taccgcggct ggattcgagc acggcgtcga tgagcttgtg
    33301 gtagggcagg acaaggttgg ccgccggcga gacccacagc cgcccggcgt agtcgatccc
    33361 gcgcgaggcg aggaaatcga gctcctcgat gaagccgaac gggtcgagga cgacgccgtt
    33421 tccgatgtag caggctttgc cggagtggat gatgcccgag gggatgaggt ggaggatgta
    33481 cttgcggtcg tcgacgacga tcgtgtgacc ggcgttggcg cctccctgga agcgggcgat
    33541 cacgtccgcc tgggcggcga ggaggtcgac gatctttccc ttgccttcat cgccccactg
    33601 acagccgagg acgatcttat tctttttggc catgtcgagg tgcccctatg tcttgcgcac
    33661 gttcgttcac gaggtcaaaa atggcgccga gcagttcgtt cttgccgagg ccgctcacgg
    33721 cggaaaacgc cagggccggg gcgttgaaga ggcgctcgac ggcggcgatt ttgcggttgg
    33781 tctggtcgcg gttgagcttg tcagtcttgg tgaccgcgac cagcgtcggg atcccgcgtg
    33841 cggcgagcca ggcgacgagc tggaaatcct gctcggtcgg gtcgcggcgg gcgtccagga
    33901 gaagcaccag tcccgcgaga tgcgggtcgc cgttcaggta gctctcgagg agtcggcccc
    33961 actcggcgcg catctgccgg gagacccggg cgtagccata gccgggcaga tccacgaagt
    34021 agaagcggct gttgaccagg tagaagttga gcgcccgcgt cttgccgggg tcggaggaaa
    34081 ctctggcgat cttgcggcgg ccgacgaggc ggttcagcag cgacgatttg ccgacattgg
    34141 agcggccggc gaaggcgatg tgggggcggc ggtcggccgg cgcctgcccg gcggagacaa
    34201 atgaacccac aaaatcgcag gtcgttatcg tgggggcggt cgccatcgca gtcctcatcc
    34261 gtcgtgtctc ttgacaccac attacgcatc catgttgtcg ggggagcagt cgcggggctg
    34321 tcccctgtga gtatagggat tcgggaggcg agtgtcaaat agtaaccggg cggtcgcgct
    34381 gccgccgccc ggcgccgtcg gccgggcgga ctcaggaggt tgctttaggc gccgcgcgcg
    34441 atgcggtagg tcgctctcac ccgcgtgcgg gtgatcgggt tccgggtcat ggccaggcgg
    34501 atgacttcgg tgaccgaacg cacgtagtgg agtttcagcc ctttcaaaag gtcggcgggg
    34561 agctcccgga tgtctttgcg gttcttctcc gggaggatca cctgcagcac tcccatgcgt
    34621 ttggcggcga ggagtttctc gttcaggccc cccaccgcga gcacatcgcc ggtcagcgtc
    34681 acctcgccgg tcatggcgac gtcggtgcgg accggggtct cggtgagcga cgagagcatg
    34741 gcgatgagga gagtgacgcc ggcggacgga ccgtctttcg gcaccgcccc ttcgggcagg
    34801 tgcacgtgga ggtcgatatc ctcgaagaaa ttctccttga gtttgaacat cgcggcgtgg
    34861 ttgcggatat acgagagcgc ggccgtcgcc gactcctgca tgacgtcgcc gagcgatccc
    34921 gtcatcgtca gtttcgcctt gccggccatg ggcgccacct cgaccgggag gacctcgccc
    34981 cccatttccg tccaggccag gccgaccgca tagccgatcg tcggttccgc tttgatgttg
    35041 ttgtcgacat acttgggggc gccgaggaga gcgtagattt tcctcttggt gaccgtcagg
    35101 cggcgcagct tccgcccctc ggcgacttcc acggccgcct ttcgcaggag cgcccccatt
    35161 tggcgctcga gttcgcggac gccggactcg cgcgtatact ggcggatgag cagcgacagg
    35221 gcctcgtcct gaaagtcgac cgcgaccccc tgcagaccga tctgctcgac cagcttcggc
    35281 aggaggaact ggcgggcgat cgcggccttc tcgaagtcca ggtagccggg gaggcggatg
    35341 atctccatgc ggtcgcggag cgcggggggg acggtggcga cggtgttggc ggtggtgatg
    35401 aagagaatgt tcgagaggtc gcactccagt tcgaggtagt tgtcggagaa cgaattgttc
    35461 tgctcggggt cgagaacctc cagaagcgcg gccgccggat cgccgcggaa gtcggtgccg
    35521 attttgtcga tttcgtccaa aaggaagacc gggttggccg agccggcacg cttcagtccc
    35581 tggatgatgc ggccgggcat ggcgccgatg tacgtgcggc ggtggccgcg aatctccgat
    35641 tcgtcatgca ccccgccgag cgacatgcgg acaaacttgc ggtcgagggc gcgggcgatg
    35701 gagcggccga cagaggtttt gccgaccccc ggcgggccga cgaggcagag aatcggaccg
    35761 cgcaccttgc ccgccaggcg aatgaccgcg aggtgttcga ggatccgctg cttcgccttc
    35821 tccaggccgt agtggtcgcc gtcgagaatg gcgcggactt ccttgaaatc gacccggtcc
    35881 tgggtgacgt cgttccaggg gagcgccagc agccactcca cgtagccgcg gatgacgccg
    35941 gcctcggccg agtacgggtg catcttggcg agcttcttga tctcctcgtc ggccttttcc
    36001 acgacggtct cggggtaatc gcggctctcc aactggctgt agaggtcgtc gacctcggcc
    36061 cccggctcgt cgaactgccc cagttcgtct ttgatcgcct tgagctgctg ctggaggtag
    36121 aactcgcgct gcgaccggga catcgactcg cgcacgtcgc cgtcaatctt gtgctcgatc
    36181 ttgaggatct cgatctcctc cttcagcagg cgggcgagtt cgagaaactg cttgcggaca
    36241 tcgaacagct cgaggatctc ctgcttggcc tccttgcgga ccaacaggtg ggatgcgatc
    36301 gtgtcggcct gctggtggta ctgctcgatg gaggccagcg ccacgagtac ctcgtcgggc
    36361 acgcgccggt tgagatggac atactcggtg aacagctcca tgacggagcg ggagagagcc
    36421 tcgagctcgc ggtcgtgcgc cggctcctcc gggggctgga cagagatgcg cacctgcacg
    36481 tagtcttcgg tccgaacata ggaggtgacg caggcgcgga cgagtccctc gacgagaacc
    36541 ttgaacgtcc cgttgggcat tttcatgact tggaggatgc gggcgacgag gccgacatcg
    36601 tagaggtcct cctgtcccgg gttgtcgaca cccgccacct tctgcgccag aagcaggacc
    36661 tggcggtccc cctccatggc ctcgcggagc gccttaaccg tgaattcccg gccgatcagg
    36721 agcggataga tcatgtgggg gaacacgacg acgtcgcgca gggggatcac gcacaggcgg
    36781 ctgttgacgg tcacgagttc gccgttgtgg acaattttga atgtcgacac cggaatcggc
    36841 ctccttacca cgggtccttg gttaccggga caggtcaaag atagcccgaa gcggtgcggc
    36901 ggcaaacacc tttttcggcg gtcggggctc ggcggggcgt ctgcgcccgg cgaacacatt
    36961 gggacggtgc ggcttaaccg gaggtcacga atgcacgctg gtgatcacca gccagaaaat
    37021 gatcagcaga gcagccaaca gcgacaacat gaggtactgg cggggagatt gcggggcgac
    37081 ctggaaaaac tcgcgggcgg gagccgcctt ctcaaaactg acaatttcgg tcagggtgat
    37141 ctgaagttcg gcggcatggc gcaggcggtc ggcggggttt ttcgccaaca gacgctcgag
    37201 caggaggacg atgtcggccg gggtcttgcg gtcgggttgc agtaggcgaa aatcagggtc
    37261 ggcgtggagc acggcttcct cgaccgcctc aaccgacgat ccggggaaag cggggcaacc
    37321 ggccaggcat tcgtagagga tggccccggc cgcgaacagg tcggcgagcg gacccacttc
    37381 ttcgccggtg acctgctcgg gcgcgcggta gcggacagcc tcggcggaag gcgagaagcc
    37441 ccggccctcc cgctccacga tcatcgtgga gaaaccgaaa tccaccaccc ggacgtcgcc
    37501 gtcgtcccgc agcaggatgt tgctgggcgt gaggctgccg tgaacaatgt ggcgcgagtg
    37561 ggcgtggttg agggcgcccg cgagcgccga agccacgcgg aggaatgcgt caatatcgag
    37621 cggaccgcgg cgcaaacgct cgcgcagcga ttccccctcg acgagctcca gggcgacaac
    37681 atagcggccg tggaacttgt ggacgccgta gagcgagcag atgttcgggt ggtcggtgtc
    37741 ggccagcgcg cgcagagtcg acagcacctg cgcccggtac gggatgtggt ccatgagatc
    37801 gaggtcgaaa agcttcaggg cgacgaatcg ctcgaggacg gtgtcccagg ccttgtagag
    37861 atcgccggtc cggctcgggg cgaggcgctc gccgagggcg tagtggagga tctggatggg
    37921 aacggactgc tccataatcg tattaacggc gaacggcggg acaaacttga gcaggtcaaa
    37981 agacgcccgg ggacggcgcg acgccgcccc cgggggaaaa aggctggtgc ccgatgatca
    38041 gatcgcgggc tgccgacgct tgcggatgac aatgaccgcg gaagcgccca ggaggacgag
    38101 caggatgatc aggccatacg tggacaatga cggcacgctg gcgttggcaa cgacagtgat
    38161 attcaccgtc gcgtcgaggc gatcaccctt cgggctgccg acgttgaaag aataaaggcc
    38221 cggatactgc ggatagataa actgctggca gccgggctgg ccaggctgca taacgcagtt
    38281 gggaggcgac catggtgtcg gatttgccgt gcccataacc cgaacggcat ccgtggcatc
    38341 attgttgaac cactccaggc tggtaaacgg caagcctttc ggaatgttaa tggtgtagct
    38401 taccggtcca ttggaccaag tccctcccgg cacaagaacc tgatacgcac cggcttcaaa
    38461 tcgcacgccg attcggtagc ccttctcctc gtcctgcgcc gtggcgatga gcgcgagggc
    38521 gaagacgcaa agaacaacaa gcacgaggcg cttcatgagg atatccctcc aattcaggtg
    38581 caagatcact ctcggatgaa cttactgcag cacgtcagtg ctgcggggaa gttgccggcg
    38641 ggggaccgcg gcgatgcggg acgaggaaga acctcccggc gagagggcgg ggggtgaata
    38701 aaaggttcca gattctccca ttcactctct cgttagttcc ccgcgcctcc gccgagaata
    38761 tcttctcaag gctccagaga atggccgaat ccaggagaat gatgaggagg gagacgatcg
    38821 caaagccgaa gagctgcgac aactccaggg tcacggcgcc cgacggggcg tggagcccga
    38881 gcaccgaggg ggcaataccc gtctggacaa tagtcagttc gaatccgacc cacaacagcg
    38941 cgagcacgag cggatggaat ccgaagcgac gattggccag accgaaaagc agcccgaacg
    39001 cacaccccac tgcgcccgcc gcggccatcc gaagccccca gaccaggggg gacagccgga
    39061 tcaggtcgag ttggccgacg ccgaccaggg tgagagcgaa aagggcgaag agacaggcgg
    39121 catcacggac ggaggcgcgg gcaacggcgg ccagaagggg agcggcggcg accaggacga
    39181 aacagccgta ggccggcgat acccgggcta caagcaccaa cagcgccgag aagccgccga
    39241 ccgcaaacaa ttcgcagccc gaccagccgc cgtggcacac cacggcccgg cgctctccct
    39301 tggcgacacg ggtacaaggc gaagaagttg gctcaatggt gtggttgtct gaacattgag
    39361 gcgaggtgcc tgcgcggagt cgatcgagac agtcgtccgt tgcgccgagg cgcaattatc
    39421 cacctccctg gaagcgggca aacccgaccg gcccgcctca gttcgacgac gaattgatct
    39481 ccgagataat ggaaaaagct ggaacccgta ctacaactgg aaaatacgcg gcctgacagg
    39541 ggctgtcaag gaaattctcg cgcaattgaa ccctgggcgc acgcaatgcg ccggattccg
    39601 ccggtcctct atggatcacc gaatcatccg ggcacctcgt gcaccggtga ccggccgcgc
    39661 cgatcccgcc gggccgagag gccgggcggc gaatgcgaat gccgtctggg acaatcccgg
    39721 gcggatggcg cgcccgggaa taggatcgct ctacttgagc gggccccggg tgactttgat
    39781 ggcgtcgacc cagatcgtgc cggagccggc gacgccgatg ttgagcttca ccagatccgg
    39841 attctctccg gctttgagga agaattgcgt gctctgggag ctccagtcga catcgcccgt
    39901 gaccgggtta tcgaggccgc gggcgaaaaa ctcgccccgt tcggggaagt ggcagagcat
    39961 ttccaggtac accatgccgg tgagattctg cgtgcgaatc ttggcttcga acgtgagcgt
    40021 ggccttctcg acatcgggat cgaccagttc cagcagcgga atggtggcgg cggcgggcgc
    40081 ctcgatccgg agagcgccgc cgccgtcggc cgtgatctcc ctgtcgagcg tgacgttact
    40141 ctgggcgatg atgccgtcga gattgtccac cgggaattcg gcgatcgttt ccacgccgcg
    40201 accgcaggcg agcaggacac acgcagcgcc gaccagtaga atcagatacc gtcgcatggg
    40261 cgaaacctca tcgatataag agtcattttg ttctgaattg tccgatcaca aagacatcgg
    40321 tattctgcca tcggcaggat cgacaggcaa gtttaatcgg gatgggcggc cagggagtca
    40381 ggcccgaggg gctctctcga gggcccgggc gagtttggag cggttctgcc ggaggaaggg
    40441 gatatccagc gacaggaaga gttttctgag tcggtcgatg acggtgggga catgggtgcg
    40501 gtcgggcaac tcaacttcga gctcgtagtc gaccgagccg tcggcgtagc gcgtggtgtc
    40561 gatttcgagg cgataggagt agtcgccgaa cctgaacgcc ttgtactggc gggtgttttc
    40621 gaagtggacg aagcggacga gagcgaggga cccgaactga tcccggacga actggatggt
    40681 gggcgtgtcc attcccatga tgtcgcgctg cagggcgagg gcttcgagag cctccccgcg
    40741 ggggatttcg gactccatct ccatgcggat agaggcttcc tcgccggccg tctcacggct
    40801 cttgatcgca agcgagccgc gctgctcgtc gacccgcacc cggagcgccc agccgtcgcg
    40861 cgacagcacg cggtcttcgg tatcaaagaa cgcgttgacc tgccgctcct cgccgtcgag
    40921 ctgccccaaa aagcccacga gcttgaggta gttggtgaag gatccgagat cgagcttgat
    40981 ctcgatttcc tcgtttgttt gtcgccgttc tgccatcctg ccgccaataa acgcaatccc
    41041 ggggcgcggt tcaatggaaa ttttgcggcc cggtcggccg gggggcggcc gcaaaaaagc
    41101 ggcgtcggat ggcgatccga cgccgcggag atctccgcca ggtcccccgg ggctcagaga
    41161 cgatagcgtt cggtgatatt gtcgttgttc tgggcgtcgt agacgcggta ctcctgcacc
    41221 gagagcgtcg tgtcgcggac gacattgatg cggaactggt tgatgcgcat gaggcggtcg
    41281 atccggctgt ggccgttttc cgacatggcg tcggccagca tcgggttgag ggcgagattg
    41341 aactcgcgga tccggccctc ggccttggcc cggagaaacc accgttcgat tttcgtggcc
    41401 atcgtctcct tcgacaggat acggcccagc ccctcgcagg tcggacaggg ttccgacagg
    41461 gcctggaggt gggaggggcg gacgcgctcg cgggtcatct ccacgagccc gaaatcggtg
    41521 accgggttga tcgccccctt ggcgcggtcg ttccggaaac agcccttgaa ctcctcgtag
    41581 agcttgcggc ggttctcctt gctgtacatg tcgatgaaat cgcagatgat gaggccgccg
    41641 atgtcgcgga ggcggatctg acgggcgatc tccttggccg cgagaagatt cgtctggaga
    41701 atggtggtct cctggtcgct cgaccccaca aagcggcccg tgttcacgtc gatcgtgacc
    41761 atcgcctcgg tctggtcgat gatgatgtat gcccccttct tgatccacac tttcctgtcc
    41821 atcatccgct cgatctcggc ctcgatgttg aactggtcga aaaggggccg ctggcccttg
    41881 tagagctcga cacggttctt caggtggggg accacctggc gggcgtaact gatcaccttc
    41941 ttgtagtcgc ccttgttgtc gacaatgagg cgctggacgt cgtcggtgaa gaggtcgcga
    42001 atcatcgaca cgatcatctc ggcctcttta tggatgagca cgggggcttt ggaggtgtcg
    42061 gccttccgtt tgagcttgct ccacagctta agtacgcgct tgacgtcggc gcggaagtcc
    42121 ggggtgtcgt gcccctcggc ctcggtgcgg acaatcaggc cgaatccttc cggacggagc
    42181 ggcgcgagca ctttcttcag gcgccgtttc tccgcccagc tcgtgatgcg cttgctcatg
    42241 cggatgtggt cgtcgtcggg gacgagcacc acatagcggc cggggatgga gatttcggtc
    42301 gagatgcggg gccccttggt cgagatcggc tccttgatca cctggatgag gacttcctgg
    42361 ccggtcttga gcacctgctc gatcgtttct ctcttgcgct tgcggatgat ctccgcaggc
    42421 gcctcctctt cgtcgatatc ctccgagtca taccggttgt cgtagtcctt gccgacgtcg
    42481 gaggcgtgga ggtaggcggc gcggggcagg ccgatatcga cgaacgcggc ctgcatgccc
    42541 ggcaggacag tgcggacaat gcctttgtag atgtcgccga ccatgcggtc gctgtcgggg
    42601 cgctccacct ggagctcgac gaggcgttcg tcctccagaa gggcgacccg tgtttcatag
    42661 tcggttgagt tgacgagaat cactttcttc atacgggatc ctgaatgttg tgcggtgtgg
    42721 ctccgaatcg ctcgcccggc tgcggctgca ggccgttgag aaaggacacc ccgtccatcc
    42781 ccttcttccc ctccggcacc agcgagtcag ctcgagggcc gagcttgcgc actggaccag
    42841 gaggcggcgc cggtcgggca cgatggcgcc gggcggcgcg cccggggccg ccggggcgtc
    42901 aacgaccggg cggctgccga gaattttcac gcggcggctg cggaaggtgg agtaggcgcc
    42961 cgggacggag ctgaggccgc ggatgaagtt gtgcaccagc ggcgcgggaa agccccagtc
    43021 gatgagggcg tcctcggggg tgactttcgg ggccggggtc gcggccgtgc cgtcctgcgg
    43081 aatgggggca aacgccgggt cggcgagccg ctccaggctg cgcagggcaa acggcccgga
    43141 caacgcggcc atccgggcat agagcgaatc gaaggtgtcg gccggatcga tcggaacgcg
    43201 ctcctggagc acgagatcgc cggtgtccac cgtatcgttg agaacaaaac tagagagacc
    43261 ggtttcggtc tcgccgttga tgagcgccca atggatcggt gcggcgccgc ggtaccgagg
    43321 caaaagcgac gcgtggatgt tgaccgagcc caggcggggg agcgcgtaga ggctgcgggg
    43381 gaggattttg aaggcgacga ccacgaaaac gtcggccttc agccccgcca gcgcctcacg
    43441 cagcgccggg ttgttcctga gcgtggcggg ggtcaggacg gtcagaccgt gccggagcgc
    43501 ctcgcgcttg acgggcgtgg ggtgcagacc cccgccgcgc ggcggacgca catcgggacc
    43561 ggtcaccacc gcagccacct catgctcgct gtgcaccagg caggccagag tggggcatgc
    43621 gtactccggc gtgcccataa acaccaatct catgcggact gtggattgcg gcggagcgac
    43681 gattctcatt ccgggaaccg tcggacaaga tcaacgcgcg ggcgaatatc gtcaggaaac
    43741 cttctgcagc tttctcaggc gcccttgaat catggtccga gtcagagcgg acatgcggtc
    43801 gatgaacagg attccctcca ggtggtcgaa ttcgtgctgg attgccctcg ccaacatgcc
    43861 gtccgttgcg atctcaaacg cgtttccatt gatgtcgata gcccgaacct tcacccaggc
    43921 cgggcgggtg atcttctgat atagtcccgg aaaggacagg caaccctcct catattccac
    43981 cggttcgccg cctgcctcga taatttccgg atttatgaag accttgaggt cggcgttgat
    44041 gtcaataacc gacaggtcga tgatgaaaac gcgcctggac acgccgatct ggggtgccgc
    44101 cagaccgagt ccctgcgcct ctttgagcgt gtcggtaagg tccgacacca agtctttgat
    44161 ttcccggtcg atcgtttcga ccggctgcgc cttctcccgc agtaccgggt cgccgtaaat
    44221 gacgatcggt cgcaccgcca caataactcc ttacactagc tgtcgacgcg cgcggcaatg
    44281 gaggatttca gaaactccat cttgacgttt tcgccgaatt tcagcacgac tcggccgcgt
    44341 tcgtcatcga tggcgaaaat cactccaaac atgccggcgg acgtgatcac tttgtcgccc
    44401 tttttcaggt ctttgagcag atgttcatgt tccttttgct ttttgcgctg cggccggatg
    44461 agcagcaaat acatgacgac gaagatgaga ccaaaccaga tgacggtcgt gatcatcgtg
    44521 ccgctgccgg ccccttccgg gcttccggcc ataagaatcg gccaattcac gtgtgtttca
    44581 actccttctc ctgttgttct atcggctggc ccgcgcggaa agcgcacggg gctgtctccg
    44641 ttaacctttc aatatagccg atccttaaca gtctgccaat tcttttgtta ccgtcatttg
    44701 aaccgcccgc ccggctgctt cgttcccgct attatggagt tgtagagtcc ggttagcggc
    44761 agccggccgc caatcggccg gccacccgat tgtccgatca tcagacccgc aaaccgtctc
    44821 cccaaggccg gcccaaacag acaaaaccca cctcaaacgg tgggtccccg gtcggtccac
    44881 atccccatgg gacgagagag tcgggatgtc tgcggcttgc gccacccggc gaagccggct
    44941 tcttctgcct gtcacctctc cggagacaat atggtgcgca caagtgtcat attctgacac
    45001 ttcccctcaa atcttctcgg acattagtcc atccggccaa actatcataa tccattgact
    45061 ggcaactact tgtccgcctc gtttcgcgac cggaccggca cggaaaacaa aaacgcggtc
    45121 ggtcaatatt ggcgcgtcat gattctccgg cgcacaaccc cggagcctcg gtttccctgg
    45181 cccggtcttg gcggcaaccg cctgggcggg ctagtgggtg tggttttgcc cgccgaagta
    45241 ggccgtccgg aactggtcga gcgtgacccg cagggagtcg atgttggcct gatcggttgt
    45301 ctgtttgcat ttcatggcgc cgacgagcag gcgatgtgtc agctcgagtt gtttctggta
    45361 gccggcatag ccctgggcgg tcggctcgag ggttttgagg cgctgggtca tgaaatactg
    45421 gctgacgatg tgctggaatt cgccggcatg gtcctctttg ttgacgaccc accgggtgag
    45481 ctggttggcg ttggcacccg gatcggccga gagctcgcgg atctgcgcca tcgacttctc
    45541 catagtcgtg atgtgttcgg ccagcaggtc gaagcgtgtg gagtcgtcat agatcccgca
    45601 cgggatttca cagtgggcgg cggccgatcc ggagccgaat gcaagtacgc cgaggacgag
    45661 agctgcgctg acgaaagttc tcaacatgat tggttcctcc aaagaaagat cgcgtgttac
    45721 agacatttcc atgcatccaa gagggtaacc gggagaatgc ggggagggtt tcctgaaagt
    45781 gcaggtaggt ccatgacaga aaaatgacac gattggccgc aaattgtgcg gccgagaaaa
    45841 aaacagtacg aaccaacgcg ggatttgctc tgtttaacgg ggaaagggat ccaacccttt
    45901 tctccatttg attgtagccc tcgtgcttga cggttgcacg ggggctatct ctttttcccc
    45961 atggacccag gcgcgcttac gcgccgcttg agtcggcagg cttctcgtct gacctgaact
    46021 tcttcgccgc ctgctggatc tttttcgcga cttcggggat cgcctcgagg agggcgtccc
    46081 actttccctt gacggccgag aggacacgga tctcatcgcg gtcgatgatg atgaacgcgg
    46141 ccggctcgac ccgggcgccg ccgccgcccc ccccgccgaa accggagccg cgcttttcat
    46201 cggttccctg tccgccgccg gcggcgaaac cgaaagagat tttcatgacc gggataacgg
    46261 tgcggtcgcc gacagtgatc gggtcgccga tgacggtttc ggatttggcg acctcgcgga
    46321 gttcgccgac cacgcccttg agaatatcgg tcacgccgtt cgacatctca gactcctttc
    46381 atctatgctc gggccaacct gattattttt cttatcggca gcctccaggc gagcaggacg
    46441 gtgcgcccgg ccaatcgata gagcggcagg gcggccgtga agcggccgga ggcgcggaac
    46501 gccgggccgt cccagacggg gacataacgg atctgcgcca gccagacggg accggcgccg
    46561 agggcggcct ggtagcagcc gaaagcgagg ccggtgtggt cggggctggc gaagccggcc
    46621 tcgatgtggg cgcggcattc ctcgacggtc gccgcgcggg agagggccgc caggtagctc
    46681 ccgagggcgc ggagaatccg cgggccgagg cggccgattt ccgccaggtc gcgccgggct
    46741 ccgcctgcgg ttcgggggga tcgtttccgc cgcggcgcgg cgggcgccgg ctctttcggc
    46801 gcggcggcgc cggcgccgaa acggaacttt ttcagcggga tgccggccag gcgaacaacg
    46861 cccgcgcggg cgccggcgtc gacttcaatg ccggtgcggc ggccgaggct cagcccgatc
    46921 agccgccggc cctcttcgaa cacgaggcgc acgcgcacgc tcagggcgag cacgccactc
    46981 ccaaggagca gcaaaagtcc tatgaccacc ggccaccagg gcatacaacc tcgaaccggg
    47041 ctacggggcg gtttcgagct cccacgcttt ccacaccagc gcgggcggaa tgctcccctc
    47101 gctcaggtag gcgtcgtgga aagcgcggag cgagaagctc tcaccttcaa tgtcgatcat
    47161 cgcgtcgcgc agcttgagga tctcaagttt cccgatcagg tacgacatct ggtaggtcgg
    47221 gcggtgggtg tagaggcgca cctggttgcg gatgtactcg cggccggaat cggtgtcgat
    47281 gtcgagggtg tcgatcatcc agtcgacgca ctcgtcgtag gtcatccggc cggtgtggag
    47341 cttgacgtcg gcgacgatgc gggcggcgcg gaagcggatg ccgtcgagga tggcgagcca
    47401 catggcgggg ttttcgccgc cgtacagccc ggcctcgtac atcatctgtt cgcagtagag
    47461 ggcccagccc tcgaccgcga ggctgttgct ctgccatttg cgcaccgggt cgtcgtgcat
    47521 gccggcgacc tgggtttgca gatggtgacc ggggtaggcc tcgtggacga ccgagccgcg
    47581 gaaaccgcgc cggtggatat agcgggcgcg caccgacaac tgcgctgaat cgagcgggtc
    47641 gggaacgggg cggacgaaaa agagagcctg ctgggcggtg tcgaacggac cggccggctg
    47701 gtaggcgatg ccggtgatca tggactgcag gtacggcggg gtttcgacga ccgcgacggg
    47761 ggcgatgttc tgggggacgg tgacgatttc gttgtcggcg cagaaggcgc gcacctgcgc
    47821 cacctcccac gcatagtagt cgaggatgtc ggcgcgcgag aaacagcgcg ggatgaagac
    47881 gcgctcgctg ccgttctggg cctcctcttc gacaaaccgc cggtactcgt cgtacgcgga
    47941 gtcggcatcg acgagcagcg cctcgcccag ggcgaggagg gagtcggcgg tgtaggggag
    48001 gaagtgctca tgggcgagct tgtagttgaa attgtcggcg ccgatggcga aggcgccggg
    48061 gtcgcccggc tcggcccgcc gcagccaatc ggaaaagccg cgcagaacct cctgcgcggc
    48121 ggcggcggcg gtgcggaatt cctcggcccg cgccggaaac tgccgcgcca tctccgcccc
    48181 ggcgtcgccg taaaagacct ccgcggagac cagggcgctc accgcctcgt caatccagac
    48241 ctgcggggga cggcggatgt ttttgcgcgc tgtctccagc agcgccggca ccgccttcat
    48301 gcgcgccagg accgacacag ccgcctgctc aggggcgagg ctccgcgaac cgagcaggag
    48361 gtacagcccg ttgacggctt catcgacgta caactgcggg agccgggtgt gccacgatat
    48421 ctgtttcaaa tcgagcagcg cggcatcgac gttcgactgg atgagccggc agcggatggc
    48481 ctggtcggcc gggaggtcgg ccgcccggta cttggcgagt ttcttgtcga acgcggcaag
    48541 cctcttgacc atgtcgcgca cggacgagga ggagtagtcc gtgaagcggt ggtcgtagtc
    48601 gtgaatgccc atttcggtgg agcggacagg gtagaaggcc tgcaaggcgg ccaggatctg
    48661 atcgcagagg ttgtcgaggc cgggttcagc ggcggccgcg gccggcgccg gaccgcacag
    48721 gaacagggcg gcggcgagcg cccagagcat gcgggtgcca atcatcgaat gcgtcctcct
    48781 tttccatccg ccaatgtacg ggggaatccg ctccggggca agaaaagtcg gggcccggcg
    48841 ccggagggct ctcagcgccg gttgaagacg ccggcgagtt caatgtgcat cgtgtggggg
    48901 aacatgtcga ccgggcggac cagcggcagg tcgtagccgg cgtcgacgat cagccgggca
    48961 tcgcgcgcga aagtcgccgg gttgcacgag acgtagagga tccggggagg gtccaggcgg
    49021 agcaggcgcc gcagagcctt ggggtgcagg ccggtccggg gaggatcgac aatgacggtg
    49081 tcgaagcgaa cgctgctccc ctcgatcagc gcgaggtagt ccttgacgtc ggcgtggtgg
    49141 aattcgacgt tgccgacgtt gttgcaggcg gcgttctcga cggccgcatc caccgcgtcc
    49201 ttcaccagtt cgaccccgac gacgtgctgg acgtagccgg ccagcaggat ggcgatggag
    49261 ccggtgccgc agtagaggtc gaacacgcgc tcggaggggc cgggccggag catctcgaag
    49321 caggtgcggt agagggtctc ggcctggatg gagttggtct gaaagaacga gttggcgcgg
    49381 atgcggaagc ggcagtcgaa gagctgctcc tcgatgtacc cgagcccgaa gagcacctgc
    49441 tcgctctcgc ctacggcgat gttcgatatc cggccgttct ggttgtgcac cagggtggtc
    49501 acttcgggaa gttcggcgcg catgtcgcgg acgaggcgct gacggtcggg gaagtcgccg
    49561 tagttggtga cgacgttgac catggtctgg ccggtccgct tgccctggcg gatgacgaga
    49621 aagcgcatgt acccggtgtg gttggcgacg tcatagacgg ggatccgggt gcggacgacg
    49681 aaatcgcgga tccagcggac gatccggttg gagagctcgc tctggagata gcagcggtcg
    49741 agatcgaaga tgtcgtcgaa ctgcccccgc cggtgcaggc cgagggtgaa cccgccgggg
    49801 gcggtgtgaa acgagaactc catcttgttg cggtagttgt agagctcggc gcagccgatc
    49861 atctcgtcga ccgccacctg ctccaggcgc cccagccggg tgaggcagtc ggccacctgc
    49921 ttgcgtttga agacgagctg ctgccggtag tcgaggtcct gccaggtgca gccgccgcag
    49981 tcgccgaagt gggagcagcg ggcgggcagg cgcatggggg agggggtgag gatgcggcgg
    50041 acgaccgcct gatcatagcg cggcttcgat ttgacgatgg cggcgaggac ggtctcgccg
    50101 gggagcccgc cgttgagaaa caccaccttg ccgtcgcggt gcgcgaccgc cttgccgtcg
    50161 aaagcaagat cggtgactac gagttcgact tcctgctcgg ccacatcggc tcctagtgta
    50221 tctcggggcg gcggcacatc ccgttggcgt cgaggcgccc gcactcctcg gtttccagct
    50281 gcatcgtgct gtggttgatg gcgaagcgct cctgcatgcc gcggctcagc cgcgcgatca
    50341 cctccggccc ccgggcaaga tcgccggggt cgacgcacac gtggcaggac agggccacct
    50401 cgcgcgacga gagcgaccag atgtggaggt cgtggacgcc gaccacggcc gggtcagccc
    50461 ggagcgcagc gatgacggca tcgtactcga tcccggccgg caccgcctca aggagaatca
    50521 tcgccgcctc cctgatcacc agccaggacg accagaagat catgacggcg atggcggccg
    50581 agaggataac gtcgaccagg taccagccgg tggcgagcat gaccaggccg ccggcgatga
    50641 cgacgaccga agagagggcg tcgtagaaca tgtggaggaa ggcagtcttc atgttgagcg
    50701 aggacgaccg ctcggcgtgg aggatccaga tcgagaagag gttgccgagc aatccgatcg
    50761 ccgccaccgt gaggaaaacc acggggtggg agatcggctg gggggcgagg aagcggcggt
    50821 aggcctcgat gagaatgaag acggcgacca cgacgagagc gacggcggag accagcgcgg
    50881 tgatcacttc gacgcggagg aagccgaacg tcgagcgctt ggtggccggg aggcgggaac
    50941 cgcgccaacc gagccaggcc aggcccagcg ccgccacgtc ggagagattg tgcacggcat
    51001 cggccagcag tgccaggtag ccggaaatca ggccgccgac aatttcggcc gccgtgatgg
    51061 cggtgttgaa gaggattgcc cagccgaggc gggcgcccga ggttgtccgc gaagcgggtg
    51121 gatgcgtgtg ggccatggcc tttccgttta ggcgttcaga ggacagtata actcatcggg
    51181 ccgacaaagc aagactccgt tgccgggagc cggcggggag agcgggatca gtcgcccttc
    51241 atgaggctga tagcccagaa tccgcccgcg agaaggagca cgaaagcgat ggcgagggcg
    51301 ttgaagtact tgtcgataaa aacctggatc cgccggccgt ggcggcggta gagcagaccg
    51361 atgaggccgg cgacgaggaa aaagcgggcc gagcggctga ggacggaggc gatcacgaag
    51421 acgggaaagg acatttcgaa gaagccggcg gcgatggtga agactttgta gggaatgggc
    51481 gtgaaaccgg cgatcccgac cgcccaggcg ccgactttca tcccccagag ctccttctca
    51541 ttgaaccaat agcgggcagt ctgcaggagg gcctcggggt cggaaccgga cagcgatgcc
    51601 gtccagctca gaatgtgtga cccgatcagt tcgaaagcgg agtggccgat gaggtagccg
    51661 agcatgcccc cgaagacgga accgagcgaa cagatcagcg cgaacttcgc ccagcgccga
    51721 tacgcgccca tgcacaaggc gatgaggaga gcgtcgggcg ggatcgggaa gaacgacgcc
    51781 tcggccagcg agaggaaaaa cagcgccgtc cgggcatggc gggactcgct ccaccggatc
    51841 acccagtcgt agagcgcgcg catccagcgg aggggagccg tgacgatgcc tgtcataccg
    51901 attcgcccgc ccggtcaggc cgtcgggacg gctagccgct ccagtttgag ccgctggagg
    51961 cggttgtcgg ccagctcgag cacggtgatc ttcatgtcgc caataataac gctgtcgtcc
    52021 ttggaaggca agtgcccaag gtggtcgatg acgagcccgg cgagagtctc ggcgcggtcc
    52081 tcgggaaggc gggcgtccat aagcacgttc accgcccccg gccgcaccgc cccgtcggca
    52141 tacgcgaccg actcggagtg gcggacgagc gggagctcgc cgcgctcgtc ctcatcgaga
    52201 atctccccca ccaactgctc gaggatgtcc tcgagcgtga cgatgccggc ggtcccgccg
    52261 aattcatcga gcaccaccgc gaacttgttc ttcttgcgct ggaaatcgcg caggagccgc
    52321 gcgagcggca gggagtcggg gacaaaaacg ggtttgcgga tgagatcccg gagcgcgaac
    52381 tcgtgcgcgt tcaggcggtg gagcacgagg tcgcgggtgt ggaggatccc cacaatgcgg
    52441 tcgaggctcc cctcgtacac cgggtaccgc gagtagccgt tgtcgatgac gacggcgata
    52501 acctgcgccg ggtcgccatc aaggggaagg ccgacgacgt cggtgcgggg agtcatggcg
    52561 cgccggacgg tggagtcggc gaaatcgaac acgttgcgga cgagctgctc ctcggtttcg
    52621 tcaaacacgc cggactgctt gccctcgaag atcatcaggt tgatctcgtc ctcggtgacc
    52681 gcctggccct ccgaatcgcg gcggatgccg agaatgcgga gaaccagatt ggccagggcg
    52741 gtgaggatcc aggagaaaaa ccaggcgatc cggatgaaga cgaggatggg gttggcgaca
    52801 tagcgggcgt agcgctccgg gttggagaga gcgatgtact tcggaaccag ttcgcccagg
    52861 accacggagg taatggtgat ggccaccgag atgagggcga ccgcgatcgg gcgcgcgccg
    52921 tcggagacaa cctcaatcgg ggagttccgc aggagctgct ccagggtgac gacgatggtg
    52981 gcgccgccga agacgccggc cagggtgccg aacagggtga tgccgacctg gatggtggcg
    53041 aggaagcggt ccggattggc gcggattttc tcggcggcgg ccgcgcccga tttcccctcg
    53101 gcgactttct gtttcaggcg gctcttccgc gaggcgatga cggagaactc ggacagggcg
    53161 aaaaacccgt tggccagcac gagaacgaga atagcgataa tctgcagcca gagggagtcc
    53221 actagcggct ccctccgcat ctactgccgg gcaacggatc gtcggcccgg tcggaggatc
    53281 tatcgcgcgc ctgcatatcc cttgtaggcg gattgctggg ccaactgccg gagtttctca
    53341 atccgctttt ccgccggcgg gtgggtcgag aagagcgacg agaggccctt gccggaaagc
    53401 gggttggcga tcatcaggtg ggccgtcgcc ggacggtcat cgagccgcat cttgaccggc
    53461 gcgcggtgga gcttgtccag ggccgaggcc aggggaaggt acttgccggt gaacccgccg
    53521 ccggcctcat cggccttgta ctcccgggcg cgcgagatcg ccatctggat gagcatggcc
    53581 gcgagcgggg cgacgatggc ggccacgagg aggccgatgg cgccgccgct gcgctcgttg
    53641 tcgcggccgc cgtagccgcc aaaaatcgcg ccccaccggg ccatcgaggc cagcatgccg
    53701 atggcgccgg cgaaggtggc ggcaatcgtg ccgacgagca tgtcgcggtt tacgatgtgg
    53761 gcgaattcat gccccaccac gccttccagc tcattctcat ccagcagctc cagcagcccc
    53821 tcggtcaccg cgaccgcggc gtgttcgggg ctccgcccgg tggcaaaagc gttgggtcct
    53881 ttgctgggga cgatgtacac tttcggcgta ggcacgcggg cggcggccgc aatgtgcttc
    53941 accaccctga acagccgggg gtggtcgctt tccgaaacct ggcgcgcacg gtacatggcc
    54001 agcacgatct tgtccgaaaa ccagtagctg acaaaattca taccggcggc gaagacaaag
    54061 gcgatgatca gcccggactg cccgccgacc agatacccga cccacatgaa cagaagcatg
    54121 agggccagca tcatgaacgt gactttcagg gtgttcacta tttccgttac ctcgctttca
    54181 atctaaaccc gctcgtgggg gtcggtcaaa tgatttcgtt tacccccaat accgtcgggg
    54241 ccgggcgaaa gttccccggc gggtctccga tccggttcgg caaccatccg cgggacaaca
    54301 tgttaaggcg aatcggcggg agccgctcaa cgcgtccgct tcctccgcgc tcgcccgaac
    54361 gagggaaccg cactcacgtc atagccatcc ttgcggtccg tcggttcggg agtctctggt
    54421 tccggcggct gcgtctggtc ggaggggcga agtccatggc gcgcgcgccc gcgcccgaag
    54481 gacgccggcc ggaattccgg ctccggctcc cccggtcctt ccttctcgtc ggagtcctct
    54541 ccctcggcgt cggccgactc cacatcgagc cgctcgcgcg aggcggccag actggcgagg
    54601 atctcgcgaa ggcggggctc cgtggcgggg gcatcgggga cgaactcatc gtcggacttg
    54661 tcggtcttcg gctccgccgg ttctgcaggc ctccagatgg gcggaggggg aggcggctcc
    54721 ggggcgaatt cgcgccgggg agcctcggcc cgcacaggtg actcgggcgc gggcgcctcg
    54781 gcgggagggg ttgcgccggg ccggtcatcg ggacgctgcg cggcgcgcac ttcgatcggg
    54841 agtggccggc ggcggatttc gggtaccgga acatcggggg cggtttcccg ggcaggctcg
    54901 cgaggtgcgg gcggctcgac gggcggggcc ggttcgcggc ttggacgacg gtcggcctcg
    54961 cgcagcgggg gcgtcaccgg ggcggcgggg cggccgcggt cgggcgccgg cgtctcaccg
    55021 ccggtcggta tggccggagg tctggggcgc tgttcgggcg gagccggacg ctcgctttcc
    55081 cgtttcggct ggcgcggcgg ctcggggcgc tcgcgccgct gctgccctcg gctcggctcg
    55141 gcttggggcg gcgatgccgg ccgcgattcc tgcttggtcg ttgcggccgg tctgggttcc
    55201 gattctctgc gcggaggctg gtcctgcggg gtgcgctccg gtttgcggcc gtggtcgcgg
    55261 tcgcgcccgc gtccgccgcg gcggtcacct tcgcgcggct cgcgcggcgg ccggcggctg
    55321 tcgcggccgc cctcggtggt ctgatgttcg cgcggcgggc gctcgcggcg gccggcgccg
    55381 cgctcccgcc gaacgggcgc ccgttcgggc gtcgtgatcc cacgggtcga ccgggcggtc
    55441 ttgaattcga atgattcgct gatcaccgcg gctccgacca tgtcccccca cacgttgaga
    55501 gcggcccggc cgcggtcgaa aagccagtcc accactacga gcaggccgat cgcattgtac
    55561 gcctcggtcg ggtaattgag cgcggcgaag agaacggcca gcgcaaaaag cgccccgccg
    55621 gtgatggagg ccgtgccgag cgagaggatg acggcgagga gaggaatggc gatgagctgc
    55681 acgatgctta gcgggacgct gtacaactgc gcggtgaaca acgccgcaat ggtcaggtag
    55741 agggccgtgc cgttggcgtt catcgaggcc cccagcggga gcacgagcgc gccggcccga
    55801 gcgtccaccc cgccctcttc gaccacgttc cggtaggtga tggggagggc ggccgccgat
    55861 gaggcggtgc cgaatgcggt cgtcagcgcc ggcacggtgg tcgcgccgta ggcgagcggg
    55921 ttgcgcttgc cgaggaattg cagcaatacc ggcaggacca ccagtccgtg caggagcagg
    55981 ccgagcagga aagtgagcat cagccaggcg agattgccgc ccaggctgcc gatcgcttcc
    56041 ggattgcggg cgatggcgga accgaccagc acgaacaggc cgaccggggc cgcgtagatc
    56101 acgaggttga ctgcctcgag gctgatatcg gcgatggctt tgaagaaatc gacgacgacc
    56161 cgggcgcggg cccccatggc cgccactatg ccggcgaaca ccagcgacag cacgaccagg
    56221 ccgaggtact gcccggcgcc gagagcggcc acgagattgt ccggaatgaa cgaggcgaac
    56281 acgtcgggca agaccgccgc gcgcgcccgg ttgaccatgt cgggaacaaa ggcgccggtt
    56341 agctgggcgc cgcggcccgg ctgaatgagg agaacgagga cagtgccgat gacaaccgcg
    56401 gcgagagtcg tcgcgagaaa atacagcacc gtcgaggcgg cggcgcgggt gacccggggg
    56461 gcgtgggcga gaccggcgac accgacaatg atgagcgccg ggatgagcgg cagcaggaga
    56521 atctggaggg cgttgacgaa cagacggccg atcacggcga tcgccagcat cacgtcggga
    56581 atgacgaatc ccgctacggc gccgaggagc accccggcca gcatgccgta gaggatctta
    56641 cgattggttt gtgggctcat tgcttccttc gagcttcatt gcatctgcgt ttacggttcc
    56701 ccggtcgcgc cgacgcctct ggcgcggcga cttgcatcgt actctccgta tgttcgctgg
    56761 gggtcgatcc gccggacaac cggcagcgat cgcgcccgga ccggggcgag tcgatttcag
    56821 cactgttatg ctaatatcac atacaactaa atagtatagc acttttctgc ccccgagggc
    56881 aacccaattc ttcaagtcct taacagttaa ttgcttacca ataagaaagc aagagaaaag
    56941 gggcggccgt acaaccggcc gccccggagg atcaaagatg gagaggtgtc aaatcagcga
    57001 aacagacgat cgatcaagag cgtgatatcc gccaccgtga tccggcggga cccgtcaaca
    57061 tcccccatga ccggatcgat cggtcccccc cgaaaaaccg ccgccaccag tgtagtgagg
    57121 tcgacaatac tcacgcggcc gtcgccgttg acgtcgccgc tgcggtggcc gaccaccgtc
    57181 agttcccccg cggcctcctc gacgcacggc tcgggggcgc tccaggaaac ggctaccggg
    57241 acggtcgctg tgtcgtagag cgcgacgagc ggcgcgatga gggggcgggc gtcgaacgcc
    57301 accgccagca cgtccccgaa gaacccatcg ggagccggcg caaccgcggc gccgagcgga
    57361 accagcgtat cgttgagccg cagactggcg gcgtcgatgt cggtgatcgc gcactcggcc
    57421 agattgccga tgtagaggct gcacacgacc gggccgagcg cgttcacctg gaaagcgtgc
    57481 agcagcgggg gctggaagcg gagcttgact ggggccaggg agtcggctgc gatcgcgatc
    57541 gcccggtggg cgtagtcgat gcgccgggtg atcgtggaac tcgcgatgca ccagagggga
    57601 gccccggtcg tgttggggaa gggaaggtcg gcggtcacgt cggccgcatt ctcatcaagc
    57661 accagccgcg actggtagac ggagtcgccg ttcttggacg cgaattcgtt gtcgttgcag
    57721 ccgtaccagt accaccggaa cagatgggtc tgtcccgcga ccgccgggtt cccgctcacg
    57781 gcgaaagtga gtttgaccag ctcgccgtcg ctcagctccc agcagagcgg ctccccgacc
    57841 gggatgatgt agtcggcgag ggcggtgatg tggaccaggc cgccggggca cgcgctgccg
    57901 tcgcagtcac tcgggatggc ggcgcgccag acgaacaact cccaggcgca ggcgctgagc
    57961 agcgagccgg gggccgcgga cagcaggcgg aaagcggtgg tgtcatactg aaagaagagg
    58021 ttgaagccgc cggtttcgaa tccgggggcg gcgttgatga gacggatcgg gatttccacg
    58081 gtgtcgcccg ggagcagggc gggcagcgaa tcctcgccga atccgagcgc gcccagcggc
    58141 tgggcgcgga cccaccccgc gcacgccgcc aaactcactg tcacgagcag cgttatgaga
    58201 cttcgagacc agacccgagg tcgatcgcgg agagagcgca taatcccttc cttctctgcc
    58261 tgttatcggt cgaaccggcc ttttgtgtgc gggacaacaa ttgtactcgc cccggccggg
    58321 attcgttggc ccgtccgctc accggccagc cgcggccggc cgacagccgg tcgtccgagg
    58381 cggtcacgga caagccgccg gggccgggcc cccccggaaa agatagccga tgagccgcgt
    58441 cgcatcggtg acgttcacgg cgcccgaacc gtcgacatcg gcgtgggcag ggcacagcga
    58501 cggcgggccg ctgcggaaca agtacgcgac catcgccgtg acgtcgccga cactcaggcc
    58561 gcccgaagag tcaaaatccc cgcggagcag acagcagtcg gggatattcc ggacgatgac
    58621 ccggaccggc accggggtca cgccgtccga agaggcctgg gcggtcacga tcggggcgac
    58681 gccggtgtag gtgcacaact gaaggccgtc gacggtcgac gggagaaagg gcgccgaatc
    58741 gacatcgatg ccgcctgagg ccagaagcgg tgtatgcccg ccgaggtggg gccagggttc
    58801 cggcatcgag ccgaacggcc cgtcgtacag caccgccccg tcgaaaacca cgtacaccgc
    58861 gcgcagactg cccgggtagg tgacgatcac cgacccgagc gcgccgtcct gcacgatcac
    58921 cgccgtgtcc gtcggcgccg cattcggacg ggggaaagga atagcgtcgc cgacaacgat
    58981 gcgggcgagg aagacgtggt cgtccagtgt cagcgggaga ccatcggcgt tgacgtcggt
    59041 ggtggcgatc tgggtcgcca cgttggcggt gaaagcagcc aggccgtaga agaagtagtt
    59101 tccgaagagc acgtagtcgg acacctcgtt ggcgatcccg ttcaggttga cgtcgccgcg
    59161 caggtcaatt tcggaagggt gggcgcgggt ccagccagcg aagaagtcga tgcccgggat
    59221 gatgtcgggg ccggcggcgc agagcgacgg ggcgccggac gcggtcggga aagcggtgtc
    59281 ggccggcgcc ggctggccga aatggtcata cacggatctc gagataaaag tcgtgtcgcc
    59341 cgagagaacg gccatgcggt tgtcgtcgca ggtgtaccag taccactcca ccgcaagggg
    59401 gcggccgatg agcgaggtgt cgttcgccac ggtgaagagg aaattggcga gggtgccttc
    59461 ggtatcggct ccgcaggtcg gggttccggt cggggaggcc aggtcggcga cggcgatcac
    59521 gcgcgccacg cccgccgggc aggggaattg cccgcactcg gcctgggcgc cggctgcgaa
    59581 ggtgaagtac tcccacccgc aggactccag caggttgccg ggctgggccg acatccaggt
    59641 gagttgcgcc gggtcatacc ggacgaggaa gtcaaagccg ccgagcgcga gacccgactg
    59701 cacctggtgc agttgggctg caaattcgac cggaccgccc agcacggggg cctcgagcgg
    59761 gcttatcact accctcaccg gagcgtcggg gggcgccgcc tgcgcatcga tccggacgct
    59821 gccgttgtag aagtctgccc cgcggatgat cgtgctctga atcaggcagg cggacggcgc
    59881 gccgaaataa gttgggaacg gatgctcctc ggttatctcc tggccgaatt cgaagacgcg
    59941 gtttgagatc aggagggtat cgccggtgga gtcggcgaat acgttgtcgt cgcagtcgtg
    60001 ccagtagaat cggaccggga actgatggtc ggcggctccg ggcgaagcgg ttgtgcggaa
    60061 acgaagccgc gccagctcgc cgggtgtatc aagggggcag tccgggtgga ccgatccgtt
    60121 gttgatctcc gcgatcgcga cgagccggac cagggcgagg ggcgaaccgc cgagcgtcga
    60181 gtcgatcccc gagcggtagg tgaagtactc ccactggcag tcgacgagca actggcccgc
    60241 gtcggcggcg agaaaggcca tcccggtctg gtcgtacgtg agcaagaggt tgaacccggt
    60301 cagagcggac gaatcatggg gggcatccat gatcaccgcg agctcgacgg tatcggcgag
    60361 ggcggcattt tcgatgctct cgacgcggat caccgccggc ccggcccaga cggccgttcc
    60421 cagggcgccg accagcaacg cgaccggcag caacctgcgg acaaacaagg agacggttgg
    60481 caggtacata caaacctccg caacaagttt tgtggtgcgc tgattcggtc cgttaggtct
    60541 cggtcgatag cgtaacgtcc gcgtcgggcg ccggctcggg ccggtcgccg ttcggcgccg
    60601 cgagaattgt tcggtcgatt attcggtcgg tagcgaagcg ggctataggc tatccagtct
    60661 gtaccagatg cgatgaacgt atctttgcat atctcgcctg agtatacgta ttccgacagt
    60721 ctctgtcaag gtcttttttg ccggcttcgg gcgggcggtt gtgattgccg aggttgcgtg
    60781 cgtggcgtat actgctgtga cgatggattt cttcgaccat accggtcaat cggacgaccc
    60841 ggaggtcccg cgggagaccg accgaaacat cccgctggcc gatcgcatgc ggccccggac
    60901 attcgacgag ttcgtcggac aggagagcgt agtcgggccg gggaaaccgc tgcgcaaggc
    60961 gattgaggaa gaccgggtca gttcgatcat cttctgggga ccgccgggaa gcggcaagac
    61021 gacgctggcg gcgctgatcg cccgcgccac caggggacag ttcgtgccgt tctcggccgt
    61081 gacctcgtcg atcaaggatg tcaaggaggt gctcaccaag gcgggcaatt actaccggat
    61141 gtcggggcac cgcacctacg tcttcatcga cgaaatccac cggttcaaca aggcccagca
    61201 ggatgccttc ctgccgtatg tcgagaaggg ggatatcgtg ctgatcgggg cgaccaccga
    61261 gaatccctcg
//
LOCUS       DAOPOE010000006        53562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_791_length_53562_cov_6.069542, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000006 DAOPOE010000000
VERSION     DAOPOE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 53562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53562
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_53562_cov_6.069542"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKM94_01685"
     CDS             <1..399
                     /locus_tag="PKM94_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65377.1"
                     /translation="DCFGQLAVGDTTVKRQLVFESTRLALEVPTGVPEAVPTLLPSGL
                     RLDQNYPNPFNAGTTIGFVCPPGAAWRLEIRDLLGRRVRDFSGRADGDAVAVSWDGCD
                     RAGSPAASGIYLCRLTVGDESRARKMLLLR"
     gene            447..1256
                     /locus_tag="PKM94_01690"
     CDS             447..1256
                     /locus_tag="PKM94_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65378.1"
                     /translation="MTWLLKVALLLLAGTAAGGPEDSLNADPLPAPPPAPAPPAEPAP
                     VFPTSLQVAIEQVRVADMRLRDTLEVTLTAHGFPIAGYAFKIAVDDRRVQIVEVLPGA
                     LYDTCRWEYFTARPLETPGRENYPRLVWNAVALAQFIPDSTQPRCYTVEGEASLLRLV
                     VSNEHLLETDDGIVPIYFFWENCGDNTLSDRSGNTLILSRRVIDYSGNPPDSAAGAFP
                     NRTGAPRQCVDPAARNQPKRLIDFHNGAVEFRLDLTADTLARQPDSAGSSR"
     gene            complement(1285..2778)
                     /locus_tag="PKM94_01695"
     CDS             complement(1285..2778)
                     /locus_tag="PKM94_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65379.1"
                     /translation="MRRFVLWVLPVVMWVGSSVKAAEAQVELTRVLESGDGITAVLPI
                     TGANGELRALAYVKDKLVLYDDLSATSLDTVAIRGQWVGRVLCRSGGDTTRIYAVSSV
                     WYEGGSLPLLTRVDYVNGEVGRDTLYLFDADDFWGPWGDMYPTVLYLDLDSASFGAGG
                     PIASYTFDRYQYIMTLGYWTWYESKSWWVSWDMGEVISYRHGLRVQPGDLRSDGSAAF
                     ATTLPYDRSHADEPGDYYHYSGTEVYFSDVFGLQTSGGGSRDLWVDDFVPDVPGDEIL
                     YYGVNQEFTGAFEGLRTFLGCYSIHDSTLSLMWLDTTLGYTPVDVFPSGRKIIGTRVT
                     ATTRYDVFIEYATGEVLGEVDLGRKTETQDFFYTGGGAELHATGRLGDSVYVYSFDVA
                     TGVSDEPDNGTKPAGFVLHQNYPNPFNGETAIQLELSATNNVTATIYNLIGQRVRLVT
                     NETLRPGTHRLSWDGTTDGGQDVASGVYLLKVQVGAASRAIRMLYLK"
     gene            2934..4124
                     /gene="sucC"
                     /locus_tag="PKM94_01700"
     CDS             2934..4124
                     /gene="sucC"
                     /locus_tag="PKM94_01700"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOD65380.1"
                     /translation="MRLYEHEGKQLFEKIGIPTAPRRVCRTPEEARAAALELGPVVVK
                     AQVLTGGRGKAGGVKLAATPEEAQHIAADLLAVKIRGYEVAAVLVEPRLDIARELYLG
                     VTIDRANYTVVVIASGEGGVDIEETAVRNPEKIVRHHLRIDEEFYGFDALAIAKQIGL
                     PSDLLKSAAPIIVNLYRLFRRCDAKLVEINPLVLTADNKLIAADARVSLDDDALFRHP
                     ELQALGIAHRHEEGEMTPRERRAAEWGIPYLDLDGDIGMFPGGAGFGIMGNDFIHYYG
                     GRPANFMDSGGGPTPERLANMLVLLDENPNVKAIFAARFGGISRCDDFAKGVIKFLRE
                     HGLSKPMVMRMTGNMWQEGVRLFEEARTQSPELFARIEIHGIETPIEEISRRAVELAR
                     GTAS"
     gene            4381..5247
                     /gene="sucD"
                     /locus_tag="PKM94_01705"
     CDS             4381..5247
                     /gene="sucD"
                     /locus_tag="PKM94_01705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOD65381.1"
                     /translation="MSILVDKNSRVLVQGITGGAGLFHTERMIAYGTNIVGGVTPGKG
                     GQTAAGLPVFDTVAECVAATGADTSVIFLPAQFVKEAAIESIRAGLKFLVVIPEHIPI
                     HDMLHVRREAVAHGATVIGGNTAGIITPGQANLGIMPDIAFTRGRVGTVSRSGSITYY
                     VADTLSRTGYGETTCVGLGGDPVLGSTFDEILLKFESDPATKAVVMCGEIGGVYEERA
                     ARVIPDMRTPVLVMIGGVYAPPGKRMGHAGAIVEGTMGTAKEKLELLADHGAHPCRTF
                     SEIPETLKKLGV"
     gene            5555..5971
                     /locus_tag="PKM94_01710"
     CDS             5555..5971
                     /locus_tag="PKM94_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65382.1"
                     /translation="MKSLITVVIAVSCLISACAAPVKMAAYPSSSWIVERDGEWCSDP
                     ANYEYIRVLANMPQAGTFEELGVIISAQESNSEFFSVSHERQIAEAQKKACQWDADAI
                     VLLSQDSDSHMSYSAFSGFGTTKEKQNRFVAIRFKK"
     gene            6036..6584
                     /locus_tag="PKM94_01715"
     CDS             6036..6584
                     /locus_tag="PKM94_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD65383.1"
                     /translation="MIDLTWKAAHLPLMPRLRHYDDLGVARFLTFSCFRHYPHFKSAG
                     MRNLILPELKALRVQHRIRLLAWVLMPDHMHLVILPPEKCDVGGLIGRFKARTVRVVL
                     GQLSQNRRRFRSDGHAALWRRWCYDRNCRPPEEVREEINYCHANPVRAGLVAQPEDWP
                     WSSGRWYAGWPGVLLDIDGVDV"
     gene            6753..8681
                     /locus_tag="PKM94_01720"
     CDS             6753..8681
                     /locus_tag="PKM94_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOD65384.1"
                     /translation="MIRFPHWLRLFVLFVAADIVISLLRRPVWTFDLAVYAAGLAMSL
                     GFFYALDLVYRLLPRALRIAWTFLVSFVLAFLLVATYFVFEQFGEYISQSMLRFIVSN
                     PSYFAAFLRTYLFNVNSLGFLVLWGLILWVWVPRARPRPVRSLRLHLPLLVVVVAVYF
                     VGLNQIIYFARGRKLPIDTSCAVAAKRLRSVVHNGLYRSERLPVAPIRNASSCNVILL
                     INESFGKRAFPTADSAVCPMPFLREWTRRESGRFFVFPTAFTNSGATDISVPSILSGV
                     APWEQSGTLHTMPLVWDWAAAAGYRTAFVSAQQFSWANLDDFFRSPGPDLYLGGDQTG
                     RPLINDTGIDEIAAMQLFCDSVLANPARRPFCAVYNSNALHTPGQTASDLLPDLTPAY
                     SSRYCNSAAVLDRAIRLLCKSLASHHLLDSTFIIITADHGDTDSLIHSQVHRLYNFYD
                     EIMNIPFLVYVPAAWLTDHPDRLAALRENEGRVVANLDILPTILAAIGANCDSANAGL
                     RARLNGASLFAPLPPERNTVALNTNDIRQWEHEGFGIFRRDRRFVYSDIEGPRWFDAA
                     DRNQTNDLWPTAPPGEKRRVLDVIDSIYHLRRMFPAAAVSEADGVGAGGAFFDRAPSG
                     LHFARASACGGLGPCSRE"
     gene            8761..9282
                     /locus_tag="PKM94_01725"
     CDS             8761..9282
                     /locus_tag="PKM94_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD65385.1"
                     /translation="MTKTIIAAGVCILACAGIWYWANRPFDPLPEGTVIDRVEVRKDE
                     RVMRLMKGGRSLREYRIALGRCPAGPKRCEGDGRTPEGGYTLDYRNPKSRFFLSYHIS
                     YPEPRDRRMADSLGCMPGGDIMIHGAAPGHAFFGRLYSYVDWTNGCIAITNPEMAEFW
                     RVVPVGTPITIYP"
     gene            9290..10042
                     /locus_tag="PKM94_01730"
     CDS             9290..10042
                     /locus_tag="PKM94_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOD65386.1"
                     /translation="MLLHIPHSSDRIPAHLRAALLLSDDELRAELLALTDAHTDTLFT
                     LGVRPATPVIFPVSRLVVDPERFIDDSREPMAGKGMGVVYVRTADGRPLRTAPTAAQR
                     EQLLAAYYHPHHRRLTELVGLELAHLGRCLIIDCHSFPSRPLPYEEDQSPDRPDICIG
                     TDSFHTPYALTAHLVAALESSGFSVALNRPFAGALVPAGYYRADTRVRSIMIEINRRL
                     YMDERTGERTARFTRIAQLLGRLLTDLQRRAE"
     gene            10158..10814
                     /locus_tag="PKM94_01735"
     CDS             10158..10814
                     /locus_tag="PKM94_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOD65387.1"
                     /translation="MKRIHKTIIPALILSSSVMAQDLAPITLPPPDTSGGKPLMEAIK
                     NRRSTREFSPDSLPPAVLSDLLWAAGGINRPESGKRTAPSAMNWQEIDIYVALPDAAY
                     RYEPKDHSLQPVLAGDIRAQTGMQPFVKDAAVNLVYVANFTRMGDPPKDEGERLRREN
                     MAYADAAFMCENAYLYCASAGLAAVVRASIDSERLAKTLSLSADQRPLLAQTIGYPKE
                     "
     gene            10984..11337
                     /locus_tag="PKM94_01740"
     CDS             10984..11337
                     /locus_tag="PKM94_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOD65388.1"
                     /translation="MATIRTISIRNPHAVNIIAGLKTIEVHTWTTKHRGRLLIVSSAR
                     PTNQGPAGHALGIVDLVDCRPFRRSDAKAAMCPWSPGQFAWILENPRRIKPFPWKGRL
                     GLYEVTWPRPRRPRK"
     gene            complement(11456..12715)
                     /locus_tag="PKM94_01745"
     CDS             complement(11456..12715)
                     /locus_tag="PKM94_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD65389.1"
                     /translation="MGRNAFAWLACAVIGLGAPGTNADWSSVTGFDGLEVTDLFVSRD
                     TLYVATPGAVHIHPAGSAAWSPTAPLGEGIEISQVIKVGGRLFAGTYGHGVYESVNNG
                     QTWQPRSAGLSGLGSLAIMAFAVRGDSLYAGTGGAAVWVFNLNGGGAWQTFRDGLGFT
                     VSWDSYSLHAWGRWLLLGAGQNAYIHRNPAGSGLWTGVEYTPFSAGGEAMWALADHDG
                     ALYGAASGGLFVSPDSGLTWTPFFGPWRPSDHGAVAATDRAVIGILSFLSHGTYVYLR
                     EGDSWTQIEHLAGVAVHDAVVFDNRVYLAHFAGLLYQPLAPTTVPEPAPRPGGFALHQ
                     NFPNPFNPATVIAYDLPSAADVNLSIYDLLGRHVATLVDSRRPAGHHRVVWQTADPSG
                     TRLPSGVYLYRLVADDRRITRKMLLLK"
     gene            13006..13797
                     /locus_tag="PKM94_01750"
     CDS             13006..13797
                     /locus_tag="PKM94_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD65390.1"
                     /translation="MDTVDITTSGSLAVVTLQRGKVNAVNGDLVDRLRAAFRDLEADP
                     AIRSVILTGRGSFFSFGLDVPELYDLPPDDFAAFLRNFTDLCRGLFLFPKPLVAAING
                     HAIAGGCILATACDRRVMATGKPRISLNELTFGSTVFQTAVELMRYWIGSRHAEEVLF
                     GGRMYTAEEARAIGLVDLAAAPERPGPSVGNRSAASEFEAVAFGEAARLGEISPDAFA
                     QAKSMLRRPVIDLIVAREEATIKRFVEIWYTPEIRASLRRITIRQ"
     gene            13904..13974
                     /locus_tag="PKM94_01755"
     tRNA            13904..13974
                     /locus_tag="PKM94_01755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13936..13938,aa:Gly,seq:ccc)
     gene            complement(14007..14618)
                     /locus_tag="PKM94_01760"
     CDS             complement(14007..14618)
                     /locus_tag="PKM94_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOD65391.1"
                     /translation="MLVPTPILKHLGGADPTLGRVIRKIGPLDITPQPGGFDRLARSI
                     MHQSLGLRAASTIIERVVTRAGGPPLTPERVAMLRERDLITCGLSRPKIRYLRGLAAA
                     VASGELRLARLARLSDEAVMEELTKVCGIGQWTAEMYLIFVLSRHDVFPVLDAGIRAA
                     VGAIYKIPPERFRAEAPLIAERWRPYRSIACRYLWEWLDSRSK"
     gene            14864..16135
                     /locus_tag="PKM94_01765"
     CDS             14864..16135
                     /locus_tag="PKM94_01765"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD65392.1"
                     /translation="MRHRVIIVGGGFGGMYAAKNLRRVPVDVLLIDRRNFHLFQPLLY
                     QVATGGLSPGDITAPLRSVLKKNRRAGVIMAEVTAVDIASRIVRTSAGDFPYDTLVVA
                     AGAAHQYFGHDEWAPLAPGLKTIEDAVDIRSRLLAAFEQAEIESDEAARRALMTFVVA
                     GGGPTGVELAGAIGEIAHHTLRGEFRRIDPGQAEIILVEGAERVLQNYPPFLSEKAVR
                     SLHKLGVTVRTRTLVTDLTPESVTLASGGVTTVVPCRTVLWAAGVSASPLGRMVVGDR
                     TDLLDRVGRVETAPDLSVPGRPEIFVIGDLASYRHQTGAPLPGLAPVAMQQGRYVARV
                     IGRRLRGKTVRPFRYFDKGNLATIGRNAAVGTYKRLRFWGYPAWLTWLFVHLMYLVEF
                     DNRLVVLVQWVWSYITRNRGARLISRADGRR"
     gene            16235..17536
                     /locus_tag="PKM94_01770"
     CDS             16235..17536
                     /locus_tag="PKM94_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOD65393.1"
                     /translation="MKILYVDPGSPLFGYVRPGYRIVSVNGREVIDTIDLRYKLAEER
                     VTIVFADPAGRELSFDFAEDEGAGESLAAGLGLTLDDSRIRTCKCDCIFCFVRQQPTG
                     MRRALYTKDEDYRLSFTHGNFITLSNMTEADLARIIEQRLSPLYISVHAADDTLRRCM
                     LRNEKLAPIVPTLRRLGGHGITMHTQVVLCPGINDGEALAQTIDELAALFPQVATLAV
                     VPVGLTRFREGQTKLRTYTREEAGGIIDYIERRQKVFRRERGSRFVWPADEFYVLGGR
                     TFPNRAEYEDMAQFENGIGMVRETITGFNRRRAMLRKVRGRQRVLFLTGESARPFLES
                     HVAAYARDQLGLRLEVEAVKNEFWGPTVTVSGLLTGQDLLRHARRRHENWDVLVVPPN
                     CLNDDDLFLDNLSLEQFRGVLAKPVLVGQYNVAASLKEAFS"
     gene            17533..18846
                     /gene="der"
                     /locus_tag="PKM94_01775"
     CDS             17533..18846
                     /gene="der"
                     /locus_tag="PKM94_01775"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOD65394.1"
                     /translation="MKLPTVAIVGRPNVGKSSLFNRFLRKYLAVVDETPGVTRDRNYA
                     VCDWAGREFRLIDTGGLVPKSGDLMNRLIYEQADFAIHEADLVLLVVDAMVGADYIDE
                     EIARRLQQSAKPTILVANKADNPRLELEVYDFLRLGLGEPMAVSATVGRGIGELLDKI
                     VALLPEAPADDVGGSGAVRVAVVGRPNVGKSSFINKLTGEERAIVTPIAGTTRDAVDT
                     PFSFEGRNYILVDTAGLRRKFKVHEDIEFYTTLRTSRAIDSAEVAVVLIDAQDGVTSQ
                     DQRILEDVVETRCAAVLAVNKWDLIEKETGTAERFARQINEALAHYSYLPLVFISALT
                     GQRVGKVLALVDQVHAENTRQLPTPELNDWLAEVTARRHPPARQGKHIKFNYITQTGA
                     APPSFVIFANYPKLVDRSYVNYLHNQLRERWGFAGVSLRLKFRRK"
     gene            18932..20269
                     /locus_tag="PKM94_01780"
     CDS             18932..20269
                     /locus_tag="PKM94_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD65395.1"
                     /translation="METRPVYDVDPEQLAASLAARIADLQDLATMGAVIASIHDIDAI
                     LSVVMDMSLRLVDGEVGAILLEKSGQLGIEISWGVQEGLIPKLPGGETAEELLPAGET
                     APPGGMDLPTRVLASGQTVILNDLSLRDEDGRRIDAVICMPIGTRDRRYGVLLILNRA
                     GGGGFNDEDRERLAMLLNFVAVAIDNARLMQEQLSRQRIEQEMAIARQVQETILPQDI
                     DAVGGVEIGASYYPAQEVGGDFYDVHALSDNEFLVFIGDVSNKGVPAALVMSAAAGII
                     KSILDRSPEIAVNELASRLNEILAREIIRDREMFITLFFARFDLARGRMTYCNGGHVP
                     GMFWSAREGTVARLSTGGPIVGQFPGAHFDLGSCPIGSGDRLFLCTDGLTEAVDKDGR
                     IFGREQAEEVFAMEIGLPPKEFCVRIREWIDRFASGAPEDSHDDFTVLQVLVK"
     assembly_gap    20433..20532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20786..21136
                     /locus_tag="PKM94_01785"
     CDS             20786..21136
                     /locus_tag="PKM94_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD65396.1"
                     /translation="MTITVETFGNVAVLRLNGRLDLASGAELKQKAKDLFAQDRTTLH
                     LNLAGVEFINSSGLGALVSTMKETRLRKGRLTLSNLASYVQEIFEITQLSHIFEIFPT
                     EEEALVSNRAAVID"
     gene            21156..21545
                     /locus_tag="PKM94_01790"
     CDS             21156..21545
                     /locus_tag="PKM94_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65397.1"
                     /translation="MPQPSILIVDDELLIRDLLYDFFTGQGWDTAIADSGEKALEILQ
                     RRSVDLVLADIRMPGIDGLELTAELRELRPGLPVVLMTGYPSVDTAVAALRHRVADYV
                     TKPFNINNLYKIVEAQVAAKEAAAKSE"
     gene            21553..21981
                     /locus_tag="PKM94_01795"
     CDS             21553..21981
                     /locus_tag="PKM94_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65398.1"
                     /translation="MKSATREPVLVVEGDESMRRRLCDYLGQLGYAVDGAGSLGEAIQ
                     MALTRPYAAAVLDVTIGPGTGEAAVSELQLNQPKMPVIVMSGAPSVRAVIAALRQGAF
                     DYIIKPYEIQDLATILPRAVERGRQELAALALTGGAAPPR"
     gene            22168..23538
                     /locus_tag="PKM94_01800"
     CDS             22168..23538
                     /locus_tag="PKM94_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65399.1"
                     /translation="MEETIMDDSVRSIAELEGGQEDTAAGGEVGRFADSYASFTRIIN
                     SLQRRYIQLEDDFTTQHRELAEANRRLTELTERNLAVTEFLNGILNSINAGVIAVDRR
                     GVITHFNSAAARILGTAAPEALGRQYRDCVRPGTPPDANALRSVERDEAIDSVERVIE
                     QPDGTRLYLSVSTTILRDAANQSAGAVEVLHDLTKIKRMEQELARLNTLAALGEMAAT
                     IAHEVRNPLSGIGGFAALLQKDLDPEDPRYRLVGNILKGVGNLNETVTTLLNYTRFEE
                     TNRTEIAYYDFLQGTVRQFERDCSERLAGARIALRPPAEGVRNPRIEVDPMLLRQAYF
                     NLFTNAAEAMAGGGEIEVGYRVYGREEAAAVWGKRLLLGLDETVLETTVADQGTGIAP
                     ENLEKIFSPFFTTRGDGNGLGLAVVWKVMKAHGGEVLAENRPAGGALFRLLLPVRRAG
                     GQGVRR"
     gene            23535..24890
                     /locus_tag="PKM94_01805"
     CDS             23535..24890
                     /locus_tag="PKM94_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65400.1"
                     /translation="MNYAVLVVDDDRLVNEFLVETLRRAGYDCTAAHSGEEALARLNE
                     RDFDIVVTDLKMPGMDGLALLAQVKKASPQTIVIMMTAYGTIDTAVKAIKNGAYDFLL
                     KPVSPETVEHIIRRVAEVIALRRENELMRKDLADKFHNIVGKSKVMKEVFDLIQGIAD
                     ARSTVLITGASGTGKELVARAIHYSSCRADKPFVKLNCAALPENLVESELFGYEKGAF
                     TDAKKTTRGRFELADGGTLLLDEISEMPLNLQSKLLRVIQEREFERVGSSQTIQVDVR
                     LVATSNRNLREYIAAGRFREDLYYRLNVIPIHLAPLEERKEDVPLLVEHFIAKYNREN
                     SRTVKGVDAQAMRLLMHYHWPGNVRELENYIERAVVTGNKEILTEDEFPAELALGRAA
                     DSLAALRVPMKLEEGNKYLILKTLEKFNGNKTRAAEALGISARTIRNKLAEYGEEVSN
                     S"
     gene            25102..25866
                     /locus_tag="PKM94_01810"
     CDS             25102..25866
                     /locus_tag="PKM94_01810"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HOD65401.1"
                     /translation="MDLATLIGLVVAFGAVGGSFLMEGGSFDAVFLAAPILIVVGGTL
                     GATTVTTSIDTVNHIPHYLRIAFFGRSHRFSDTIEQIVKLGEKARREGILGLERDMER
                     IRNPFFRKAVRLVIDGTETSALREILETEIAYLEARHKRGITFFQKAGGYSPTMGILG
                     TVLGLVHTLGNTSDASRMAAHIAAAFIATLWGVGLANLFFLPVSDKLRMRHEEEIAHL
                     ELIMEGIMALQSGENPRHIRTRLLAFIAPIHRNHEE"
     gene            25869..26615
                     /locus_tag="PKM94_01815"
     CDS             25869..26615
                     /locus_tag="PKM94_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOD65402.1"
                     /translation="MRRFRRRVEDKENLDRWLLTYSDLITLLLAFFVVMYSMSQVDAK
                     RFGQMAKALHGILKGGENVFDYDEAKPTEDGHGLLKLGNLRMLQARVEERFKGINRLD
                     DVSSEITERGLVIHIVDRALFDEGAADLKPQAIDVLDLINDEVRAMPNHLRIEGHTDD
                     RQIATLRFPSNWELSAARATEVVRFFVNQHDFPPDRISALGYGEFRPIRPNNSIENRA
                     MNRRVDIVILTMELSLKEPTSQLYFTAANE"
     gene            26794..27201
                     /gene="flgB"
                     /locus_tag="PKM94_01820"
     CDS             26794..27201
                     /gene="flgB"
                     /locus_tag="PKM94_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOD65403.1"
                     /translation="MKIADFLFKSTGVHRFEAYLDVSSFRHKLISSNVANVSTPGYGA
                     RDIDFQAEYTRLTGETGRVAGELTHPAHLPLGAHRDREPDIEETRPKPDELNAVDIDR
                     EVSQMAQNELQFTVAARLLQKKFEGLRRAIAGR"
     gene            27213..27707
                     /gene="flgC"
                     /locus_tag="PKM94_01825"
     CDS             27213..27707
                     /gene="flgC"
                     /locus_tag="PKM94_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOD65404.1"
                     /translation="MAGILGAIEIASRGLSVQRAKMNATAENIANAETTQTPEGGPYR
                     RKRVQVREDETGVSFRQAMRRAGVSLERTNTRHFPARGVCVQSEVEVPDVDMKEIRDP
                     QSSYRLIHDPEHPDADEQGYVKMPDIEVVNEMVDMMAAARAYEANTVTIAAAKQMAKD
                     ALDI"
     gene            27725..28039
                     /locus_tag="PKM94_01830"
     CDS             27725..28039
                     /locus_tag="PKM94_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65405.1"
                     /translation="MKINPTAIQSYQQLVQRQPQRPDEQARPADAARELTIAPQAAAS
                     GSDLAVKAPEGSYADFLTEAERAALELLFQRFGDSGRFGAALGGEAAESRVLGKLIDV
                     KV"
     gene            28041..28361
                     /locus_tag="PKM94_01835"
     CDS             28041..28361
                     /locus_tag="PKM94_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOD65406.1"
                     /translation="MTAPVNGLTRLVPGLVARLEPTGKTVPLPEMKAEDGFGEMLSDF
                     VSGVNSLQYESAQIQQAFLEGEPVELHQVMVKAEEAGVAAELLIEIRNKLVEGYQQLI
                     QMPL"
     gene            28423..29985
                     /gene="fliF"
                     /locus_tag="PKM94_01840"
     CDS             28423..29985
                     /gene="fliF"
                     /locus_tag="PKM94_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOD65407.1"
                     /translation="MEQVRQFFRNLAAWVGRLSPGQVMMLLGIAAGTIVGTILLVGWV
                     NTVTYARLYSDVDEAEAGEVVAYLNDNKIPYELSDGGRTIAVPSDQVYKTRIALASEG
                     LPRNGNIGYSIFDKNNLGMTDFLQNLNFRRALEGELTRTIMQLSEVQAARVHIVMPSD
                     RLFKQDQKQATASVVLKLRSGGLTKQQINGISHLVAASVEGLRPENISIVDYDGNLLS
                     SGQPTDELAGLTASQLDVRKQAETYLEHKAQSMLDDVLGPGRSVIRVTAELDFNQTER
                     TSETYDANQPAVRSEERTKQTNASTDKAAEASESQEQGSTETTITNYELNKTVEHIIN
                     GIGSIKRLSVAVMVDGVYTPAAAEDGGAAMSYQPRSQEELDRLAAIVRNAVGFDSQRN
                     DQIEMVNIAFDRRDLIEDQQALDSMYRRDFYLEIAKKVGLVLLALLVFLYLRRKVSRL
                     FQSIGAMAAAPKRTPARPLTPGAAPEAAAEEGPRVIPEQRRTRLVDEMQDAARKQPEE
                     IARAIKTMMSDV"
     gene            29985..31004
                     /gene="fliG"
                     /locus_tag="PKM94_01845"
     CDS             29985..31004
                     /gene="fliG"
                     /locus_tag="PKM94_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOD65408.1"
                     /translation="MGALKYESMTPLQKAAVALVAFGPEVSAHVLKGMPEADLEKITI
                     EIANLREVPPDVEDKVIDECYQIFMARQYISQGGIDFARKILESAVGVAKAREIMQRL
                     ETTIKSSGFSLLKDIDPKQLTSFLQNEHPQTISLILTQLSPQHAAAVLSELSPELQGE
                     VALRIAMMEKIAPEILHEIEQTLSGHFDAGAAAQMSASGGAKAIAEILNLIDTTAEKN
                     ILQSLEADDPDLAAEVKNMMFVFDDLTLLDDRSIQRLLKEVETKDLAIALKAASDEVK
                     NKIFSNVSERVAVMIREEMEFMGPTRLSDVEAAQGRIVEAVRRLEEEGQIIVSGRGGK
                     EDIIV"
     gene            30997..31698
                     /locus_tag="PKM94_01850"
     CDS             30997..31698
                     /locus_tag="PKM94_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOD65409.1"
                     /translation="MSKVLRHVRPAPTVCVGERQLDHDRQSRAVAVLGRLFPAVAIVT
                     DPGGARFIPVEEAFTMEDACRAAHEEEYRRGYREGQQAGLAQGLAKAEQVLSRFDAAI
                     ADAIEQRHRLLDEARREILDLVVQISRKVTFDAVTIDPESTLRLINGVIDTLLDRSRI
                     RIKVHPGHLPIVEQSIAQFLGGSALIKELTIEPDPRVREGGCLIETPHGDVDARLESQ
                     FEVVREVLLAEEAGQ"
     gene            31716..33011
                     /locus_tag="PKM94_01855"
     CDS             31716..33011
                     /locus_tag="PKM94_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOD65410.1"
                     /translation="MYAERIRAAHTIRHYGKVMQVVGLVIESAGPAVSIGKLCTIENH
                     DTGAKIRAEVVGFRDNRILLMPLGPITGITPGAIVTSTAEHLRVPVGRELIGRVLGGL
                     GQPIDGKGPLGTGAWRQLSGEPIPALKRRRITEPLRTGIRAVDLVTAVGKGQRLGIFA
                     GSGVGKSIMLGMMARGTSADVNVLALVGERGREVREFVESDLGPEGMRRSVVVAVTSD
                     QPALIRIKGALVATAIAEHFRDQGMDVLLLMDSLTRIAMAQREIGIAIGEPPTAKGYT
                     PSVFALLPGLLERAGQAEKGSITGLYSVLVEGDDFNEPVSDAVRSILDGHVALSRRLA
                     ARNQYPAVDILDSVSRLMKSVCTPEQVELAGQVRELLSVYREAEDLINIGAYVRGSNP
                     KIDRAIAKMDALDAFFRQGIHEISDFDQSVAQLRQLLAD"
     gene            33029..33484
                     /gene="fliJ"
                     /locus_tag="PKM94_01860"
     CDS             33029..33484
                     /gene="fliJ"
                     /locus_tag="PKM94_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOD65411.1"
                     /translation="MKKFRFRLEALLKQREQIEKDRRRELAEAMRRTARQKQALDGLI
                     ERSRETLAHKRARQTGTLAVAELQLCARFLHRLKRETLGADEVLRALRKTEAKKREAL
                     LEAAKNRKIYDTLKDHRRSEFYHEAEQRERKETDEIGLNVFRAGRGKPA"
     gene            complement(33556..34317)
                     /locus_tag="PKM94_01865"
     CDS             complement(33556..34317)
                     /locus_tag="PKM94_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65412.1"
                     /translation="MQSRIARACLLCLAGMVLLAPAAAAQDPGIPDTVHVDSIGAFTN
                     TRAIVPVNFYNDEPLGGLEVTLHFSSPDVHVDSFSFAGGRLQSYSVKGTYTPSGGFSI
                     YCVPFAGEPTIPAASGLLGRLYLSWSVGISPQLVSIDTITIVNVDVSYSTRFSTASAV
                     AFTPKYRRGWVKIDQGFGCCIGTRGNVDGDPTDAVTLPDLTRLIGILFRGMAPADCPE
                     EANINGDATPQPTVADITYLIAYLFRGGPPPAPCQ"
     gene            34614..34826
                     /locus_tag="PKM94_01870"
     CDS             34614..34826
                     /locus_tag="PKM94_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65413.1"
                     /translation="MPEEMTPRTVFRCTYIRACYLADELKCFGYKTDCPLYRKCNGEY
                     SDEAAFHEAMDRLIDRTRAVYAGLDK"
     gene            35081..36271
                     /locus_tag="PKM94_01875"
     CDS             35081..36271
                     /locus_tag="PKM94_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65414.1"
                     /translation="MFRLIAVVMVSALVAAAAPAAGTPGRGSVPTVTVLPGAGDTLPV
                     TPTYEWIDIYCGEPTLDGAPLAPGDTIFVYDPDNMCCGAAIVHADGMYGFAPVYRDDA
                     YSAEDEGAEPGDTLRFVVNGVPVAAAPPVIWTANGESFAVCRFLTAPPAYREVSLDIK
                     PGSCPNPLNLVGPSDNGKAVLPAAVLGAPDFDVRTINPPTLALGGAEVVRWSYEDVGR
                     PKADPYDTCACHEYGPDGYMDLTLKFYRAEVAPAVSFAADGDYAPLVLTGSLLDGTPL
                     SGVDCVRVQRGGRPEGNSGGGPTGKVQAPADFALANHPNPFNPSTQITFALPQAGPVT
                     LRVYDVQGRLVRTLANGPFDAGVHSVEWNGTDGTGSAVASGVYLYRLSAGETVVTRKM
                     ILMK"
     gene            complement(36376..37695)
                     /locus_tag="PKM94_01880"
     CDS             complement(36376..37695)
                     /locus_tag="PKM94_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65415.1"
                     /translation="MAKILIVDDEESIRSSLKAALERRGHEVVTAESYARGETFMSGR
                     FDLIFLDVLLGDGNGIDLLKAALEKNPRHIIVMFSGHADIDMAVRATRAGAYDFIEKP
                     LSLDRVLVTVDNAVRTRRLQAEADRLALRAYGEFVGESPEILKLKSDILRAAPKAARF
                     LILGENGTGKELVANMIHRASGRSNGPFVAVNCAALPSELVESELFGHVRGAFTGARA
                     DRRGRFVEADQGSIFLDEISEMPAGAQAKILRVIETREVTPVGASRTYSVDCTIIAAS
                     NRDLETMAADGSLRQDLLYRLNVVTFHLPPLRERTQDIPLLADHFLARFAGETGSVPK
                     RLERDSLKLLCAYSFPGNIRELKNLMERVNIYCERSVVTVADLKPLLPAAARGGRRSL
                     KEAMAEFEREYLEAAIARHGGNMTEAARELGIERSLLYKKLRRLEEG"
     gene            complement(37688..39322)
                     /locus_tag="PKM94_01885"
     CDS             complement(37688..39322)
                     /locus_tag="PKM94_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65416.1"
                     /translation="MTFTGRIRLYLILVAVVPTVLVVSVIYLLGMRQAAAAERRSAQH
                     SLDRFAQYQRAVADELRQAVIAAAATPSAARALLLLKAGRASDVRFDAVPAGLDFFEI
                     ADSSLLVQASARRPGLIGERLPSLDSLAADSAPLASVEYDLNGPHAALAWRLPAESAY
                     VVYAGRYLDSQYTGTVARLIGADVRIVLAGDTGGGLSGMDPGRLYAERDSLVAVLAGG
                     DAAGWFLAASFRSGPEQRVFGNLLVVSGLVALGSVLAAVALGLYISGRAQREFDNLLT
                     ATARVSGGDLSTPVMAYEEGEFAQLADSFTAMTFRLRDAQRRLATTEKIAAWQAVGRK
                     IAHEVKNPLTPIVISTDDLRRSFQEQLPDFPRILEETTATIKSEVERLTRLLDEFVGF
                     ARMGPPVIRDVAPARLLEPLAALYRGETGTGRLTLSNRSARRSVRLDPEMMHQVLVNL
                     VKNGLEASPESRVAVALGDEPGALVITVADTGPGFARDLLERGLEPRVSTKKGGSGLG
                     LVICQRIVHDHGGAIDLQNRADGGALVTLRLPVEHG"
     gene            complement(39319..40560)
                     /locus_tag="PKM94_01890"
     CDS             complement(39319..40560)
                     /locus_tag="PKM94_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65417.1"
                     /translation="MTRCSWIFALPLLLAAVCAAQPGDSLWIESRPLQVKLDRLYLDC
                     GLEARVFPDCRFVVHRGPDSLYSGCIESSYPGVAVSSPVGGFFDTLRLADLRVWLERA
                     PYDTLAPIVLGQVGLLPAPLAGVGDSTAGGRVVFRPYASHFEASLAFEDQVIDGFFSY
                     RRYTAGGEDVASVSRPAPFFVALVPNPARAIAQRGFLSTSLYYRLDPQRLAMIFEGDE
                     LRAFNRCGVGGDTAARPYPYAPGPGRALLNNLRGPSRSVVIAVEHPMFEPAAGYFADI
                     LSRDRFRPTVTSTLAGADLRLVAVPLGESEADGLRCLSGLLALDTLAGSAAAQSLQAV
                     SSCLDSAALAAEPAAALRYVQLAESVLRDDLGVFPLFRPTLYFTARRSLLGEVFDRRG
                     GIDVSGLARVRIPQNRKDCRP"
     gene            40822..41928
                     /locus_tag="PKM94_01895"
     CDS             40822..41928
                     /locus_tag="PKM94_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65418.1"
                     /translation="MKTKRILEMMAIAMAVAVFTLTGCSGDGKAEKPNQAAPAAEAGA
                     MPSTIAGAELLGASRPAVTGEKGFPIADMIRVDSTLYAVAGNELIITDLKTKSERRVS
                     AAEGLYAVTEHNGKIYAGGTALYELGETGLEPAAAAFDGTIRALTGHEYRLMIGTSTG
                     LFASSIFGDEALFEGIDVTAMAGDSDELWVGTGGQGLYRWDGAEFQRRYLLRDTAVFD
                     FINALAFAHGHLFVGTDEALYVYDGGRWETVTTDDGLPSNRIRSIDASGWVVYLGTPA
                     GVASWFNYDVAPVARLEDRAATLVRTFGRKLIVATEREGVLLKAGPVVSTLIRAETIP
                     VLAEAAPQFSEPLPAGALVDSADAVGAQPVAAAK"
     gene            complement(42133..42384)
                     /locus_tag="PKM94_01900"
     CDS             complement(42133..42384)
                     /locus_tag="PKM94_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOD65419.1"
                     /translation="MLNILWYIIVGFLAGLVARAILPGADHMGFFATTAVGIVGGILG
                     GLIGDRFSKTPSAAKFRPAGFIMSVIGAVILLIVLRLLR"
     gene            complement(42899..43174)
                     /locus_tag="PKM94_01905"
     CDS             complement(42899..43174)
                     /locus_tag="PKM94_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65420.1"
                     /translation="MVSGTKKFYLTTMTDNTSDEFEIRNLRLTAMFFPTAYGSVEATG
                     SSADTPEHSELYDARGAVEAERAAELEAQRRLIEAKVQGSARNKSDK"
     gene            43535..44266
                     /locus_tag="PKM94_01910"
     CDS             43535..44266
                     /locus_tag="PKM94_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOD65421.1"
                     /translation="MIKGLFRSASGMLPAIKRQETIANNVANANTAGFKKDAVFTKEL
                     SRAQRKLVPVKSDWEQPMVDEVYTNFEGGSFSRTDNPLDLAIDGDGFFTVQLPDGRTA
                     LTRSGAFSINRDGLMALADGSTLLAEGGPVEVGNGKLTVTAAGQIEVDGSAVGQVRPV
                     TTADVGALEKIGASLFAVPEGTELIPVEHAEIRQGYVEESNVDIVREMIDMIVAFREY
                     EANARAVTTQDQSLDNLFRRVGGDG"
     gene            44344..45132
                     /gene="flgG"
                     /locus_tag="PKM94_01915"
     CDS             44344..45132
                     /gene="flgG"
                     /locus_tag="PKM94_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOD65422.1"
                     /translation="MIKAMRTAATGMVAQQMNVDNIANNLANVNTTGFKQAKIEFQDV
                     LYQNLRRAGTATAIGTEVPTGLAIGYGTRASATVRQYAEGNLTPTGNPLDLAIDGDGF
                     FQVQMPDGTTAYTRDGAFKISGDGQIVTSDGYFLLPQVSIPQDATALAVGTDGTIEVM
                     VAGQSDPVEIGQIELARFVNPAGLLAVGRNLLTPTRASGDALTDVPTQSGLGQIDQGY
                     LEMSNVKVVDEMVNMIVAQRAYEMNSKAIQTADDMAQIANNLKR"
     gene            45138..45863
                     /gene="flgA"
                     /locus_tag="PKM94_01920"
     CDS             45138..45863
                     /gene="flgA"
                     /locus_tag="PKM94_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOD65423.1"
                     /translation="MTELIRKIILILLFLAFAAAGGAVPVTQAAIDELVRMYGLDTTM
                     YAIDVDLNRLHTTEVGTIPATAPGAAAEPSRLTLRPLSAKEPLGLFTAEATVEQGGAP
                     VESAMIRFRITKFAQVLVAADRIDMRAPIAPEQVEVRRVDVTTMYEKPLTAVGELAGM
                     RAARMIRKGTVLTTACVEIPPDVESGRTVEIVYRDGQCRIATRGTALQAGRVGEAIKV
                     RNDSSGKVITARVADAGAVTVEP"
     gene            45876..46463
                     /locus_tag="PKM94_01925"
     CDS             45876..46463
                     /locus_tag="PKM94_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOD65424.1"
                     /translation="MKLLKALIILLILLLLPYSLKLKGQDFGQSRSLFTDVKANRVGD
                     LLTVLIYENNTAQQNAQTKTEKSTEASTSGGPGVGSLDFIKLFGADAKNESTFDGKGS
                     NSRNGSLRARMSVTVVEVRANGDLVIEGSRTIGINGERETLHLAGVVRQKDVTPQNTV
                     DSYLIGDAEIHYTGKGSLNSGTRPGIFTRIINWLF"
     gene            46474..47610
                     /locus_tag="PKM94_01930"
     CDS             46474..47610
                     /locus_tag="PKM94_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOD65425.1"
                     /translation="MVGTMCHRLATPTGLVIALAILLAWATTAEANKVRIKDIADFRN
                     EQQVDLIGYGLVIGLDGTGDGTGTQFTVQSLVNMMQRMGMTVDVEKVKVKNVAAVMVT
                     GRITSSQTEGGLIDVTVSSIGDAKSLQGGTLLMTPLSGLDGTVRAVAQGPVSIGGFNI
                     QVDESNKLYNNYTLVGRVPNGGKVTAPPTPENATEREIYLSLRSPDYTTAHRVAERIN
                     IKYGLLAYPVDAGSVRLVVPDSLSHPTARIEFLSDVGQLQVVPDNPARVVINERTGTI
                     VAGEHVTIDAVAIAHGSITVNIESTPVISQPEPFSQGETVVTSDARIQVQDEKARVVE
                     LKPAVYLSDVANALNRIGATPRDIIAIFQALKQSGALRAELVIL"
     gene            48246..48650
                     /locus_tag="PKM94_01935"
                     /pseudo
     CDS             48246..48650
                     /locus_tag="PKM94_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            48681..49169
                     /gene="flgN"
                     /locus_tag="PKM94_01940"
     CDS             48681..49169
                     /gene="flgN"
                     /locus_tag="PKM94_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOD65426.1"
                     /translation="MVDRLIDIIGREAALFERFLLLLERQQRALVANDQEELTRVTDL
                     QRETLAESRRLHEEREGVLADIRRTNAIEGDLTVTRLVAMVDADRADRLAALRGTILD
                     LNERILRLRNQNVLLLNRSREYIRRTMELLSRIASPRAETYAPAGAAGAEARTVALDR
                     RV"
     gene            49169..50560
                     /gene="flgK"
                     /locus_tag="PKM94_01945"
     CDS             49169..50560
                     /gene="flgK"
                     /locus_tag="PKM94_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOD65427.1"
                     /translation="MSGLMAGLEIGKRALLSHQVNLQTIGHNIANVNTPGYSRQRVTI
                     SSTYPSETAAGLVGTGVTVEDIRHVRDLFLGDQYRQENKTLGQWTYKQKILSQVESLF
                     AEPNDNTLADRLNEFWDSWDDLSKNPNATNREGVIGAARLLTESFHTLARDINKLRDS
                     VDADLATYTSQINQTTGEIAALNRQIVTQEVDGSRASDLRDRRDQLIDELSSLLDVNT
                     IEDANGAVRVMVGAMEIVTGLDTIPIATRILNDNGRMTHELVWRNTAVELKNVNGELK
                     GLLDTRDKIIPGYLQELGALARTLVEQVNAVHRTGYGLDGSTGLNFFDSRYTDAESIR
                     INEDLVAAPHRIAASASGEVGDGSITLAVQNLRNERVMASGTTTINDYYSSLVGQLGV
                     EAHQATSFAENSALLVHQIESARQAVQGVSLDEEMANMVKFQHAYDAAARVITTMDEA
                     LDTVISRMGVVGR"
     gene            50601..50783
                     /gene="csrA"
                     /locus_tag="PKM94_01950"
                     /pseudo
     CDS             50601..50783
                     /gene="csrA"
                     /locus_tag="PKM94_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            50965..52884
                     /gene="flgL"
                     /locus_tag="PKM94_01955"
     CDS             50965..52884
                     /gene="flgL"
                     /locus_tag="PKM94_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOD65428.1"
                     /translation="MRISTNMLNDRVVFNMQRSLARFLRLETQMSSGRRINQPSDDPV
                     GTQRDLGYRTELAKGEQYRSNIEQAQTWMQSYDTILGDLKDIVSSAKEIAVAMANGVY
                     DDVTRNGAAAEVESLYERILQLATSELEGRQVFSGHRTTTKALTSTATGVVYRGDTGQ
                     IDYQIEASARLTVNLIGSEVFLRQLRPVGAEADLDAGVSGGTLVADLHNGAGIDTAPG
                     TFTITDLNLGLTSTIDVSGAATLNDVIAAINAQLAADGITSLTARPGAEGNNLMLDTS
                     ETGLISAATALARLNSGNGVDLQPGQIRVTDGAGINEVIDFSGAATVGDIIAAFNAHM
                     SAAGYGGVTMGLNAAGTGLAITDAGAPPLGLRIEEVAPDQTTAANLGLAGTVGSLLVG
                     ADLAPTAAFRVAETTGTTATDLGLAGDITGDFAGNDLDPRLQPTTPLASLQHGLGLGE
                     GEIVLRQGERTRTIALDAPGMVTVQDLIDALNGCGLDITAAINAGGRGIQIDNNDSTR
                     SLVVADVGNGRAAKALGVWGGADLMGSMLLLIESLRADDQEGTGRLLQNLDDGIEHLL
                     NQRASVGARDIRLERTHSRLTDLELGFTKLLSEVEDADMTKLVTDLATYENNYQAALM
                     ASARIIQPSLLDFLQ"
     gene            52909..53376
                     /locus_tag="PKM94_01960"
     CDS             52909..53376
                     /locus_tag="PKM94_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOD65429.1"
                     /translation="MVVQTERFGPIEAPEEKLIRLQKPILGFEGCATFFLVEQDEFRP
                     FLWLQSADRPDLAFIVINPLLVRPDYRIEVHAEEVRDLEITSLRQVETYVIVTVPENP
                     RDISVNLQGPIVINRGNNFAKQLVLVNSSYSVRHRLLPAADEEPVRERETVEV"
BASE COUNT         9907 a        17569 c        17302 g         8684 t
ORIGIN      
        1 gactgcttcg gccaactggc ggtcggcgac actactgtga agcggcaact ggtcttcgag
       61 tccacccgcc tggcgctcga ggtgcctacc ggcgtcccgg aggccgtccc gaccctcctg
      121 ccctccggcc tccgcctgga ccagaactac cccaatccct tcaacgccgg caccactatc
      181 ggtttcgtgt gcccgcccgg agcggcgtgg cggttggaga tccgcgacct cctcggccgc
      241 cgggtccgcg atttttccgg ccgggccgac ggggacgcgg tggcggtctc gtgggatggc
      301 tgtgaccggg ccggctcgcc ggccgcctcc ggcatctatc tctgccgcct gaccgtcggg
      361 gacgaaagtc gcgcgcggaa gatgctcttg ctgcggtagg ccaaataacg ctgtgctttc
      421 cacccgccgc agcgtatctt cacgccatga catggctgct gaaagtcgcg ctcctgttgc
      481 tggccggcac cgccgccggc gggccggagg attcactgaa cgccgatcca ctcccggcgc
      541 cgcctccggc gccggctccc ccggccgagc ctgccccggt cttcccgacc tcgctgcagg
      601 ttgcgatcga gcaggtgcgc gtggccgaca tgcggctgcg cgataccctt gaggtgacac
      661 tgaccgccca cggctttccg atcgcgggct acgctttcaa gatcgccgtc gacgaccgcc
      721 gcgtgcagat tgtcgaggtc cttcccggcg ctctctacga cacctgccgc tgggagtact
      781 tcaccgcgcg cccgctcgag acgcccggcc gcgagaatta cccccgcctc gtctggaacg
      841 ccgtcgccct cgcccagttc atccccgaca gcacccagcc ccggtgctac acggtggagg
      901 gcgaggcctc gctcctccgc ctggtcgtgt ccaacgagca cctgctggaa accgacgacg
      961 gcatagtccc gatctacttt ttctgggaga actgcggcga caacacgctc tccgaccggt
     1021 ccggcaacac cctgattctc tcgcgccgcg tgatcgatta ctcgggcaac ccgcccgatt
     1081 ccgccgccgg cgcctttccc aaccgtaccg gcgcgccgcg ccagtgcgtc gatccggccg
     1141 cccgcaacca gcccaagcgg ctcatcgatt tccacaacgg cgcggtcgag ttccgcctcg
     1201 acctgactgc agacacgctt gcccggcaac cggattcagc cggttcatct cgatagtgcc
     1261 ggatcaggta gggtcgtagc agctctactt gaggtagagc atccgaattg cgcgcgatgc
     1321 cgccccgact tgaaccttca gtaggtacac tccgctcgcc acgtcctgtc cgccgtcggt
     1381 agttccgtcc cacgatagcc gatgcgttcc tggtcgcagc gtttcattgg tgacgagccg
     1441 cacccgctgg ccgataagat tgtagattgt ggccgtgacg ttgttcgtgg cggaaagctc
     1501 cagttgaatc gccgtctcgc cattgaacgg attcggatag ttctgatgga gcacaaatcc
     1561 cgcgggtttc gtcccattgt cgggctcatc agatacgccg gtcgccacgt cgaaagagta
     1621 gacgtacacc gaatccccca gacgtcccgt tgcatgcagt tctgctccac ctccggtgta
     1681 gaagaagtcc tgagtctctg tcttccgtcc cagatcgacc tcccccaata cctcgcccgt
     1741 tgcgtactct ataaacacat cataccgcgt cgtcgccgtg acgcgtgtac cgattatctt
     1801 gcgccccgag gggaaaacgt caaccggcgt gtatccgagc gttgtatcaa gccacatcag
     1861 gctgagtgtg ctgtcgtgga tactgtagca gcccaaaaat gtgcgcagcc cctcgaacgc
     1921 cccggtgaac tcctgattca cgccatagta cagaatttcg tcaccgggaa catcgggcac
     1981 gaaatcatcc acccacaaat ctcggcttcc cccgccgctt gtttgcagcc cgaagacatc
     2041 cgagaagtac acttccgtcc cgctgtagtg atagtaatcg cccggctcat ctgcgtggga
     2101 cctgtcatat ggtagggttg tcgcgaatgc cgccgaccca tcgctccgca aatcgcccgg
     2161 ctgtactcgc aggccgtgac ggtaggagat cacctcgccc atgtcccatg acacccacca
     2221 tgacttcgac tcgtaccagg tccaataccc cagtgtcatg atgtactggt accggtcaaa
     2281 cgtgtaactg gcgatcgggc cgcccgcgcc gaaactcgcg ctgtccagat caaggtatag
     2341 caccgtcggg tacatatccc cccacggtcc ccagaaatcg tcggcgtcga acaggtacaa
     2401 cgtgtccctc cccacctctc cgttgacgta atcaactcga gtcagcagcg gaaggctgcc
     2461 tccctcgtac caaacgctcg acaccgcata tatccgcgtc gtgtccccac cgctccgaca
     2521 caacacgcgg ccgacccact gcccgcgaat ggcgaccgta tcaagactcg tcgccgacag
     2581 gtcatcataa agcaccagct tgtccttgac atacgcgagc gcccgcaact ctccgtttgc
     2641 cccggtaatt ggcagcaccg cggtgatacc atctccggac tccagtaccc gcgtcagctc
     2701 aacctgcgcc tcggccgcct tgaccgaact ccccacccac attactaccg gaagaaccca
     2761 cagaacgaat cgtctcatca gaatacctcc gcctgccctc aataaacccc ctccgcccca
     2821 tttcccaacc cagaaatcga caccccttga cctcccgctc ccgactccct acattatccc
     2881 gcaaataacg cgaacatcgg cccctgcgcg cccctttact aaggaccctg accatgcgcc
     2941 tctatgaaca cgaaggtaaa caactgttcg agaaaatcgg tatccccacc gccccccgcc
     3001 gcgtctgccg tacaccggag gaggcgcgcg ctgccgctct ggaactcggc ccggtggtgg
     3061 tcaaagccca ggtgctcacc ggcgggcggg gcaaagccgg cggcgtcaaa ctcgccgcca
     3121 cccccgaaga ggcgcagcac atcgccgccg acctcctcgc cgtgaaaatc cgcggctacg
     3181 aggtcgccgc cgttctggtc gaaccccggc tggacattgc gcgtgagctg tacctcggag
     3241 tgaccatcga ccgggccaat tacaccgtcg tcgtcatcgc ttccggcgaa gggggggtcg
     3301 atatcgagga gaccgccgtc cgaaacccgg agaaaatcgt gcgccaccac ctccgcatcg
     3361 acgaggagtt ctacgggttc gatgccctcg cgattgccaa acagatcggc ctcccctcgg
     3421 atctcctcaa aagcgcggct ccgatcattg tgaatctcta ccggctcttc cgccgctgtg
     3481 acgccaagct cgtcgagatc aacccgctgg tgctcaccgc cgacaacaaa ctcatcgccg
     3541 ccgacgcgcg cgtgtcgctc gatgacgacg ccctgttccg gcaccccgaa ctgcaggccc
     3601 tgggcatcgc ccaccggcac gaggaggggg agatgacgcc gcgcgagcgc cgggcggccg
     3661 aatggggcat cccgtacctc gacctcgacg gcgacatcgg catgttcccc ggcggcgccg
     3721 gtttcggcat catgggcaac gatttcatcc actactacgg cggccgcccc gccaacttca
     3781 tggactccgg cggcggcccc actcccgagc gcctcgccaa catgctcgtc ctgctcgatg
     3841 aaaaccccaa cgtcaaagcc attttcgccg cgcgcttcgg cggcatctcc cgctgcgatg
     3901 atttcgcgaa aggggtcatc aaattcctgc gcgagcacgg cctctccaaa ccgatggtga
     3961 tgcgcatgac cggcaacatg tggcaggagg gcgtccgcct gttcgaggag gcccgcactc
     4021 agtcgcccga gctttttgcg cgcatcgaga tccacggcat cgagactccc atcgaggaaa
     4081 tctcccgccg tgcggtcgaa ttggccaggg ggacggcctc atgagcaggg gagaggccgc
     4141 agccgggcgc ggcgccttgc gcgtgcgagc agcgctgaga ggagcaggat cgatggccgg
     4201 gcaaaggccg acacgccggc cggattctct tggccgacct gcgttccggc agcaccacca
     4261 cactccggtc tcccggactc cgtctcctcc tgtaggtcag gccccttgtg gtcctgacat
     4321 ctttcgccga aaccgcgcga tccgccctct ccacccgtcc cgtcgcaaag gagcccgctg
     4381 atgtccatcc tcgttgacaa gaactcccgc gtcctcgtcc aggggattac cggcggcgcg
     4441 ggcctgttcc atacagaacg catgatcgcc tacggcacca acatcgtcgg cggcgtcacc
     4501 cccggcaaag gcggccagac ggccgccggc ctccccgtct tcgacaccgt cgccgagtgc
     4561 gtcgccgcca ccggcgccga cacctcggtc atttttctgc cggcgcagtt tgtgaaagaa
     4621 gcggcgatcg aatccatccg cgccggcctc aagttcctcg tcgtgatccc cgagcacatt
     4681 cctattcatg acatgctcca cgtgcgccgg gaggcggtcg cgcacggcgc caccgtcatc
     4741 ggcggcaaca ccgccggcat catcaccccc ggccaggcca acctcggcat catgcccgac
     4801 atcgccttca cccgcgggcg cgtcggcacc gtctcccgct ccggctcgat cacctactat
     4861 gtcgccgata cgctcagccg caccggttac ggcgagacca cctgcgtggg cctcggcggc
     4921 gacccggtgc tgggctcgac tttcgatgaa atcctgctca aattcgagag cgacccggcg
     4981 accaaagcgg ttgtcatgtg cggcgaaatc ggcggcgtct acgaagaacg cgccgcccgc
     5041 gtgatccccg atatgcgcac cccggtgctg gtcatgatcg gcggtgtgta tgcgcccccc
     5101 ggcaaacgca tgggccacgc cggcgccatc gttgagggga ccatgggcac cgcgaaagag
     5161 aagctcgagt tgctcgccga ccacggcgcc cacccctgcc gcacgttttc ggagattccg
     5221 gaaacactga agaagctggg cgtatagagc tgtgggttac ccctttctcc ccctcccaaa
     5281 atgtgattgc cccggcgcgg cagccggggt attatctaat gcagcgcgat tgctgaagct
     5341 cggtggggcg tcccgcgcgg gcaggccgat tagccagttc gggggccacg ctgctcgggt
     5401 cgcacatatc tcctgaaaca gacggcgggg aggtgcagcc aaggacgctc gtaccataga
     5461 tcgtggacca ccggtgagag tcggataact tcaagcagga cattcgacag aagatctgag
     5521 aaatggtcag gaagagtcga cgggaggatc agcaatgaag agcttgatta cagtcgtgat
     5581 cgcggtctcg tgcctcatca gcgcctgtgc tgcacctgtc aagatggcag cctatccaag
     5641 ttcaagctgg attgtcgagc gtgacgggga gtggtgcagc gacccggcca attacgaata
     5701 cattcgcgta ctggctaata tgccacaagc tgggaccttt gaggaacttg gtgtgatcat
     5761 atctgcacaa gaaagcaatt ccgagttttt tagcgtgtcg cacgagcggc aaatagccga
     5821 ggcgcagaaa aaggcttgtc agtgggatgc tgatgctatc gtcctgctct cgcaggattc
     5881 agattcccat atgagctatt ctgctttcag tggttttggg acaaccaaag agaaacagaa
     5941 ccgcttcgta gccatacgat tcaagaagtg aaaccagcat cactgcccgc cggacacgtg
     6001 gcccaccatc tatggtgggt ttctcttttt gaaatgtgat tgacctcacc tggaaggccg
     6061 ctcatcttcc actcatgccg cgcctccgac actatgacga tttaggcgtc gcccgttttc
     6121 tcaccttctc ctgctttcgc cactaccctc atttcaaatc ggccggcatg cgcaatttga
     6181 tcctccccga actgaaagcc ctccgggtgc aacatcgcat tcgtctcctg gcatgggtgc
     6241 tgatgcccga tcatatgcac cttgttatcc tcccgccgga gaagtgtgac gtaggcggtc
     6301 ttatcggccg attcaaagct cgtactgtcc gggtggtgct gggacaactt tcccagaacc
     6361 ggcgccgctt ccgttcagat ggccatgccg ctctttggcg gcgctggtgc tacgatcgga
     6421 attgccgtcc gccggaagaa gtgagggagg agatcaacta ctgccatgca aatccggtga
     6481 gagccggtct ggtggcgcaa ccggaggact ggccatggtc aagtggccgc tggtacgccg
     6541 gctggccggg cgttttgctt gacattgacg gcgtcgacgt gtaatagaga ggcccccacc
     6601 ataaatgatg ggccaccgga gttacccggt cgccgtcccg gcgctcaccc tccggtaacc
     6661 gagcattcgc agccgccttc cgctcactat cctgcgatac tcctcctgaa cgccgcttgc
     6721 ctcccggccc cctattcctt accctacccg gcatgatccg tttcccgcac tggctccggc
     6781 tgtttgtgct tttcgtggca gccgacattg tcatttcgct cctccgccgc ccggtgtgga
     6841 cattcgacct tgcggtctac gccgccggcc tcgccatgag cctcggcttt ttctatgcgc
     6901 tcgacctcgt gtatcgcctg ctgccccgcg ccctgcgcat cgcctggacg ttcctggtct
     6961 cttttgtgct cgctttcctg ctcgtcgcaa cctatttcgt tttcgagcag ttcggcgagt
     7021 acatctcgca gtcgatgctc cgctttatcg tctccaaccc ctcttacttc gccgccttcc
     7081 tccgtacgta tttgttcaac gtgaacagcc tcggcttcct cgtcctgtgg gggttgatct
     7141 tatgggtgtg ggttccgcgc gcccgcccgc ggccggtgcg ctcgctgcgt cttcacctgc
     7201 cgctgctggt tgtcgtggtc gccgtctatt ttgtcggcct caaccaaatc atctatttcg
     7261 cccgggggcg caagctgccg atcgacacct cctgtgcggt cgcggccaaa cgcctgagat
     7321 cggtggtcca caacggtctc taccgctccg agcgtctccc ggtcgccccg atcaggaacg
     7381 cctcctcctg caacgtcatc ctgctgatca acgaatcgtt cggcaagcgg gcgtttccga
     7441 ccgccgactc ggcggtctgc ccgatgccct tccttcgcga gtggacgcgg cgagagtccg
     7501 gccgcttctt cgtcttcccg accgccttca ccaactccgg cgccaccgac atcagcgtgc
     7561 cctcgatcct ctccggcgtc gccccctggg agcagagcgg cacgctgcac acgatgcccc
     7621 tggtctggga ctgggcggcc gcggccggct accgcacggc cttcgtgagc gcgcagcaat
     7681 tcagctgggc caacctcgat gacttcttcc ggtcccccgg tcccgacctc tatctcggcg
     7741 gggaccagac cggtcggccg ctcatcaacg acaccgggat cgatgaaatc gccgccatgc
     7801 agcttttctg cgacagcgtg ctggccaacc ccgcccggcg gccgttctgc gcggtgtaca
     7861 attccaacgc cctgcacact cccggccaga ccgccagcga ccttctgccc gatctgactc
     7921 cggcctattc ctcccgctac tgcaacagcg cggccgtgct cgatcgcgct atccggcttc
     7981 tctgcaagtc gctcgcaagc catcacctgc tggacagcac gttcatcatc atcaccgcgg
     8041 accacggcga caccgacagc ctgattcact cccaggtgca ccggctctat aatttctacg
     8101 atgaaatcat gaatatcccg tttctcgtct atgtcccggc ggcgtggctg accgaccacc
     8161 ccgaccgcct tgccgcgctg cgcgagaacg aggggcgggt ggtcgccaat ctcgacattc
     8221 tcccgaccat cctcgccgcg atcggggcga attgcgactc ggccaacgcc ggcctgcgcg
     8281 cccgcctgaa cggcgcgtcg ctctttgcgc cgcttccgcc ggagcgcaat acggtcgctc
     8341 tcaataccaa cgatatccgg cagtgggagc atgaggggtt cggcattttc cggcgagacc
     8401 ggcggtttgt ctattccgat atcgagggtc cgcgctggtt cgacgccgcc gatcgcaacc
     8461 agaccaacga tctctggccg acggcgccgc cgggggagaa gcggcgcgtg ctggacgtta
     8521 tcgattcgat ttaccatctc aggcggatgt tccccgccgc cgccgtctcg gaggcagacg
     8581 gcgtcggagc aggcggggcg ttcttcgatc gcgctccaag cggcctgcat tttgcccgcg
     8641 cctccgcatg cggcggtttg ggaccgtgca gtcgggagtg atgggccggg ccgtggggat
     8701 gtcggatacg aacgaccgca cctttcgccc cggatccgga ggcaacccgc gacagccatg
     8761 atgacaaaga ccataatcgc cgcgggagtc tgcattctgg cgtgtgccgg gatctggtat
     8821 tgggcgaacc gccccttcga cccgctgccc gagggaacag tgatcgacag ggtagaagtc
     8881 cgcaaagacg agcgggtcat gcggctgatg aaaggtggac ggagcttgcg ggagtatcgg
     8941 attgcgctcg gccgctgccc cgcagggccg aagcgctgcg agggggacgg ccggaccccg
     9001 gagggcggat acacgctcga ttatcggaac ccgaagagcc gtttcttcct gtcctaccac
     9061 atctcctacc cggagccgcg cgatcgccgg atggcggact cgctcggctg catgcccggc
     9121 ggggacatca tgatccacgg cgctgcgccc ggtcatgcct tcttcgggcg cctctactcg
     9181 tatgtggatt ggaccaacgg atgcattgcg atcacgaatc cggagatggc cgagttctgg
     9241 cgcgtggtcc cggttggcac tccgattacc atctatccct gaggctgcga tgttgctcca
     9301 tatcccgcac tcctcggacc gcattcccgc tcacctgcgg gcggcgctgc tgttgtccga
     9361 tgacgagctc cgggccgagc ttttggctct caccgatgct cacaccgata ctctcttcac
     9421 gctcggcgtt cgcccggcca cgccggtgat tttcccggtg agccgcctcg tggtcgaccc
     9481 ggagcggttt atcgatgact cgcgggagcc gatggcgggg aaggggatgg gggtcgtgta
     9541 tgtgagaacg gccgatggcc gcccgctgcg tactgcgccc accgccgccc agcgcgagca
     9601 gttgctggcc gcttactacc atcctcacca tcgccggctc accgagcttg tcggcctgga
     9661 actggcgcac ctcggacgct gcctgatcat tgattgccac agttttccct cgcggccgct
     9721 gccttacgag gaggaccaaa gcccggaccg ccccgacatc tgcatcggca cggacagttt
     9781 ccatacgccg tatgctctca cggcgcacct ggtcgccgca ctggagtcga gcggtttctc
     9841 ggtggcgctc aaccgtccct tcgccggagc gctcgtccct gcgggctact atcgggcgga
     9901 tacgcgggtg cggagcatca tgatcgagat caaccggcgg ctctatatgg atgagcgcac
     9961 aggggagagg accgcccgct tcacgcgcat cgcccaactc ctcggccgcc tcctcaccga
    10021 cctgcagcgc cgtgcggagt agggcggagc gcccgacaca acggctcgcg atcgcggaga
    10081 aagtgccgcc ggtgccttgc ccttcttccg gcggtctcgt atattcccgg cgcgaacagt
    10141 tcaacctgga ggttcttatg aagcgcatac acaagaccat catccctgcc ctgattctca
    10201 gttcctcggt catggcgcag gaccttgccc cgatcacgct ccccccgccc gacaccagcg
    10261 gcggcaaacc gctcatggag gcgatcaaga accgtcggtc gacccgcgag ttcagcccgg
    10321 attcgctccc gccggcggtt ctgtccgatc tcctctgggc ggcgggcggc atcaaccgcc
    10381 ccgaatcggg caaacgcacc gctccgtcgg ccatgaactg gcaggagatc gacatctatg
    10441 tcgccctgcc tgacgccgct taccgctacg agccgaaaga ccactcgctc cagccggttc
    10501 tcgccggcga catccgcgct caaacgggta tgcagccgtt cgtcaaggat gccgcggtca
    10561 atttggtcta tgtcgccaac ttcactcgca tgggggatcc ccctaaagac gagggcgaac
    10621 gcctccggcg cgagaacatg gcctatgccg atgccgcctt catgtgcgag aacgcctacc
    10681 tgtactgcgc ctcggcgggc ctggccgcag tcgtgcgcgc ctctatagac agcgagcgac
    10741 ttgccaaaac cctgtcgctg tcggccgacc agcgtcccct cctggcccag acgatcggtt
    10801 acccgaagga gtagccacgg gcatgcaacc ccataaaggc ggcccgccgt ttggagttgg
    10861 tctgtctccg ggatcgttta ggtcaggacc ctggcggccc tgacgttctt gccgtcaacg
    10921 gaaccggtcg ggtcaggatt cttgtgggcc tttcatccgt cacttgacac aagggaatac
    10981 ccgatggcta ccatcagaac gatcagtatc aggaacccgc acgccgtgaa cattattgcc
    11041 ggcctcaaga ccatcgaggt ccacacctgg accaccaaac accgcggtcg gctcctgatc
    11101 gtttcgagcg cccgcccgac aaaccaaggc cctgccgggc atgccctcgg catcgttgac
    11161 ctcgtcgatt gccgcccctt ccggcgctcc gatgccaagg ccgccatgtg cccatggtcc
    11221 ccggggcagt tcgcctggat cctcgaaaat ccgcggcgga tcaaaccgtt cccgtggaaa
    11281 ggtcggctgg gcctttacga agtgacatgg ccgcgccccc gcaggccgcg aaagtagccg
    11341 gccggcagtc cgcaaattcg catgggatcg aaaggcggcc ccgatgacga gatcccgtcg
    11401 ggccatcgca cggctcgcct gatcccgggc gagaggggag ggagcgggag tcagctcact
    11461 tgagcagcag catcttgcgt gtgatccggc gatcatcggc cacaagccga tacaagtaga
    11521 cccccgaggg caggcgggtg ccggatggat ctgcggtctg ccacaccacc cggtgatgtc
    11581 cggccggccg gcgggaatca accagggtcg ccacgtgccg gccgaggagg tcgtagatgc
    11641 tcaggttgac gtcggcggca gacggcaggt cataggcaat aaccgtggcc ggattgaacg
    11701 gattggggaa attctgatgc agcgcgaaac cgcccggccg aggcgctggc tcggggaccg
    11761 tggtgggagc aagcggctgg tacagcagcc cggcgaagtg ggcgagatac acccggttgt
    11821 cgaatacgac tgcatcgtgg accgctaccc ccgcgaggtg ttcgatctgc gtccaggagt
    11881 ccccctcgcg gagatacacg taggtgccgt gggagaggaa gctgaggatt ccgatgaccg
    11941 cgcggtcggt cgccgcgacc gcgccgtgat cggacgggcg ccacgggccg aaaaacggcg
    12001 tccaggtcag gccgctgtcg gggctcacga atagcccgcc ggaggccgct ccgtagagtg
    12061 cgccgtcgtg gtcggccaat gcccacatcg cctcgccgcc cgcgctgaag ggggtgtact
    12121 cgacgccggt ccacagaccg gaaccggcgg ggttgcggtg gatgtaggcg ttctgaccgg
    12181 cgccgagcag cagccagcgt ccccacgcat gcagcgagta ggagtcccag gagacggtga
    12241 accccaaacc gtcgcggaac gtctgccagg cgccccctcc gttcaggttg aacacccaaa
    12301 ccgcggcgcc gcccgtcccc gcgtacaggc tgtcgccgcg gacagcgaag gccatgatgg
    12361 ccagcgatcc caggccgctc agccccgcgc tgcggggttg ccaggtctgc ccgttgttga
    12421 ccgattcgta gacgccgtgg ccgtacgttc cggcaaagag gcgccccccg accttgatga
    12481 cctgcgagat ctcgatgccc tcgccgagcg gggcggtcgg cgaccacgca gccgatccgg
    12541 ccgggtggat gtggacagcg ccgggagtgg cgacatacag ggtgtcgcgg gacacaaaca
    12601 aatcggtgac ttccagtccg tcgaaaccgg taactgagct ccagtcggcg ttcgtgcccg
    12661 gggcgccgag gccgatgact gcgcaggcga gccatgcgaa agcgttccgg cccatgtcaa
    12721 atcctcttcc aatacgattc ccatgcggga tacgggcgaa gggtacggga gtttccgtag
    12781 ccgcgtcaaa atagagtgta tagaccgaag ataatgcacc gcgggactat tggagcggcg
    12841 ctcggccggg ggagatccgc cttccgggcc cccgccggcc cccgccgatg ggaatccagg
    12901 ccggccgagg gcgggcgagg agcccggcat cctgagtgcc gtcccaaccg gaactctacg
    12961 ccggaactgc ttgccccacc gccccccgct agcgtaattg aaagtatgga caccgtagac
    13021 atcactacct ccggctccct ggcggtggtc actctccagc gcggcaaggt gaacgcggtc
    13081 aacggcgatc tcgtcgaccg ccttcgggcg gccttccgcg acctcgaggc cgacccggcc
    13141 atccgctcgg tcattctgac cggccgcggt tcgtttttct cgttcgggct cgacgttccc
    13201 gagctgtatg atcttccgcc cgatgacttc gccgcctttc tccgcaactt cactgacctc
    13261 tgccgcggtc tgtttctctt tcccaagccg ctggtggcgg cgatcaacgg ccacgcgatc
    13321 gccggcggct gcattctggc caccgcctgc gaccgtcgcg tcatggcgac tggaaaaccc
    13381 cgcatctcgc tcaacgagtt gacgttcggg tccaccgtct tccagaccgc cgtggaactc
    13441 atgcggtact ggatcggctc gcgccatgcc gaggaggttc tcttcggcgg ccgcatgtac
    13501 accgcggagg aggcgcgtgc gatcggtctt gtcgatctcg ccgccgcgcc cgaacgcccg
    13561 gggccgtcgg tcggcaaccg cagcgccgcc agcgagttcg aggcggtcgc attcggcgag
    13621 gcggcccgcc tcggggagat cagtcccgac gcttttgcac aggccaagtc gatgctccgc
    13681 cgaccggtca tcgacctcat cgtcgcccgc gaggaagcca ctatcaaacg attcgtggag
    13741 atttggtaca cgccggagat ccgggccagc ctgcgccgga tcacgatccg ccagtaacgg
    13801 cccgctcccc ccgatttatg tcggcaaggg agttgacaac ccggcgcggc cggcatagat
    13861 tagatcgcct tcaggccgaa gcgaccggct gaacggttcc aaggcgggcg taattcagtg
    13921 gtagaatgca agcttcccaa gcttgatatg tgggttcgat tcccatcgcc cgctttttct
    13981 tatggccggc ggccggcagc ccgaagtcat ttcgaccggc tgtccagcca ctcccacaga
    14041 tagcggcagg cgatggaacg atagggtcgc cagcgctcgg caataagcgg cgcctctgcc
    14101 cggaaacgct ccggagggat cttgtagatt gcgcccacgg cggcccggat gcccgcgtcc
    14161 agcaccggga agacgtcgtg gcggctaagc acgaagatga ggtacatctc ggccgtccac
    14221 tggccgatgc cgcagacttt cgtgagttcc tccatcaccg cctcatcaga caggcgcgcc
    14281 agccgggcga ggcgcagttc gccggaggcg acggccgccg cgaggccccg aaggtagcgg
    14341 attttgggac gcgagagacc gcaggtgatc aggtcgcgct cccggagcat tgcgacccgc
    14401 tcgggcgtga gcggcggccc gccggcgcgc gtcaccaccc gctcgataat cgtcgacgcg
    14461 gcccggaggc ccagcgactg atgcatgatc gaccgtgcca gtcggtcgaa cccgcccggc
    14521 tgcggcgtaa tatccagggg accgatcttc cgaatgacgc gtccgagcgt cggatccgcc
    14581 ccacccagat gcttgagtat cggtgtcggg actagcatgg cccgaagata gggattcaat
    14641 cggccgcggg caagtgagga gatgtagcca aaagggaccg tcgtttgatt ttcggggccg
    14701 acccggccct tgcgtggact gactggacct cgttttcggc cgaatccggg ccccggcaac
    14761 cgggaaccgg gcgaggcagc aaaccattat agcagtctga agctcccggg accgggtccg
    14821 ggtgcggtcc aacctggagg ctccaagcat ggagaaccgg cacatgcgcc atcgagtcat
    14881 aattgtcggc ggggggtttg ggggtatgta tgcggcgaag aacctccggc gcgtcccggt
    14941 cgacgtgctc ctgatcgatc ggcgcaactt ccatctcttt caaccgcttc tgtaccaggt
    15001 cgctaccggc gggctgtccc ccggcgatat cactgctccg ctgcgttccg tgctcaagaa
    15061 gaaccgccgg gccggcgtca tcatggccga agtgaccgct gtcgacatcg cctcccggat
    15121 tgtgcggacc tcggcgggcg actttccgta cgacaccctg gtggtggccg cgggcgcggc
    15181 tcaccagtat ttcgggcacg acgagtgggc gcccctggca ccgggattga agacaatcga
    15241 agacgccgtg gacatccgca gccgcctgct ggcggcgttc gagcaggccg agatcgaaag
    15301 cgatgaggcg gcccgccggg cgctcatgac cttcgtggtc gccgggggcg gaccgaccgg
    15361 cgttgaactc gccggggcca tcggggaaat cgcccaccat accctgcgcg gagaattccg
    15421 ccgcatcgac cccggccagg ctgagattat cctggtcgaa ggcgccgaac gggtcctcca
    15481 gaattacccg ccctttctct ctgagaaggc ggttcggtcg cttcacaagc tcggggtgac
    15541 tgtgcgcacg cggactctgg tcacggacct cactcccgag tccgtcaccc tggcctccgg
    15601 cggcgtcacg accgtggtgc cctgccgcac cgtcctgtgg gcagccgggg tgtcggccag
    15661 tccgctgggc cggatggtgg tgggggatcg gacggacctc ctcgaccggg tcggacgcgt
    15721 ggaaacggcc cccgacttga gcgtgcccgg tcgtcccgag atctttgtga tcggcgatct
    15781 ggcgagctac cggcaccaga ccggcgcacc gctgcccggc ctcgctccgg tcgcaatgca
    15841 acaggggcgc tatgtcgccc gggtcatcgg acggcgcctc aggggaaaga cggtgcggcc
    15901 gttccgctac ttcgacaaag ggaacctggc gaccatcggc cgcaacgccg cggtcggcac
    15961 ctacaaacgc ctccgtttct ggggttaccc cgcctggctg acctggttgt tcgtccacct
    16021 gatgtacctg gtggaattcg acaaccgcct ggtcgttctc gtgcagtggg tctggagcta
    16081 catcacccgc aaccgcggcg cgcggctgat cagccgggcc gacggccggc gttgacttcg
    16141 gccccccgat ttcgttcctt aggtcgctcc cggggagaag cggcgcgccc gaccggcgct
    16201 ccgaccgagg tgatgtctgc aaggtgaatg tgcgatgaag atactctatg tcgatccggg
    16261 atcccccctg ttcggctacg tccggccggg ataccgcatc gtgtcggtca acggccggga
    16321 agtgatcgac accatcgacc ttcggtacaa actggcagag gagcgggtga cgatcgtctt
    16381 cgccgacccg gcaggccggg agctgtcgtt cgatttcgcc gaggacgagg gggcggggga
    16441 gagcctggcg gccgggctgg gactgacgct cgacgactcc cggatcagaa cctgcaagtg
    16501 cgactgcatc ttctgcttcg tgcgccagca gccgaccggc atgcggcggg cgctctacac
    16561 caaagacgag gactaccggc tgtcgttcac ccacggaaat ttcattaccc tctccaacat
    16621 gaccgaggcc gacctcgcac ggatcatcga gcagcgcctg tcccccctgt acatctcggt
    16681 gcatgcggcc gacgataccc tgcgccggtg catgctccgc aacgagaaac tcgcccccat
    16741 cgtcccgacc ctccgccgcc tcggcggcca cggaatcacc atgcacaccc aggtcgttct
    16801 gtgcccgggg atcaacgatg gagaagcgct cgcgcaaacg atcgatgaac tggccgccct
    16861 ctttccgcag gtggcgacgc ttgcggttgt cccggtgggg ctcacccggt tccgcgaggg
    16921 gcagacgaag ctgcgcacgt acacgcggga ggaggcgggc gggatcatcg attatatcga
    16981 gaggcgccag aaagtgttcc ggcgcgaacg cgggagccgg tttgtgtggc cggccgacga
    17041 attctatgtg ctcggcggcc ggacgttccc gaaccgggcg gaatacgagg acatggccca
    17101 gttcgagaac gggatcggga tggtgcggga gacgatcacc ggcttcaacc gccgccgggc
    17161 catgctccgg aaagtccggg gccgacagcg cgtgctcttt ctcaccggcg agtcggcccg
    17221 gccctttctc gagtcgcacg tggccgcgta cgcccgggat caattggggc tgcggctgga
    17281 ggtcgaggcg gtcaagaacg aattctgggg tccgaccgtc accgtgtcgg gcctgctcac
    17341 cggacaggat ctcctgcggc acgcgcgccg gcggcacgaa aactgggacg tgctcgtcgt
    17401 gccgcccaac tgcctgaacg acgacgatct gttcctggac aacctgagcc tggagcagtt
    17461 ccggggcgtg ctggccaaac cggtgctggt cgggcaatac aacgtcgcgg catcgctgaa
    17521 agaggccttc tcatgaaact cccgaccgtg gccattgtcg gacgcccgaa cgtggggaaa
    17581 tcgtcgctct tcaaccggtt tctccgcaaa tacctggccg tggtggatga aacccccggc
    17641 gtcacccgcg atcgcaacta cgcggtgtgc gactgggccg gtcgggagtt ccgcctgatc
    17701 gacaccgggg gcctggtccc caagtccggc gatctcatga accggctgat ctacgagcag
    17761 gccgatttcg ccatccacga agccgacctc gtgctgctgg tggtcgatgc catggtcggc
    17821 gccgactaca tcgatgaaga aatcgcccgc cgcctgcagc agtccgccaa acccaccatc
    17881 ctggtggcca acaaagccga caatccccgg ctggagttgg aggtgtatga tttcctccgc
    17941 ctcggcctgg gcgagccgat ggcggtctcg gccaccgtcg ggcgcggtat cggcgagctc
    18001 ctcgacaaaa tcgtggcgct gctgccggag gcgcccgcgg acgacgtcgg cggttccggc
    18061 gccgtccgcg tggcagtcgt ggggcggccg aacgtgggca agtcctcgtt catcaacaaa
    18121 ctcaccggag aagagcgggc cattgtgacc ccgatcgcgg gaacgacgcg cgatgccgtg
    18181 gatacgccgt tttccttcga agggcggaac tacattctcg tcgacaccgc cgggctgcgc
    18241 cggaagttca aagttcacga ggatatcgaa ttctacacga cgctgcggac gagccgggca
    18301 atcgacagcg ccgaggtcgc cgtagtgctg atcgacgcgc aggacggcgt gacgtcgcag
    18361 gaccagcgca ttctcgagga tgttgttgaa acgcgctgcg ccgcggtgct ggcggtcaac
    18421 aagtgggacc tgatcgagaa ggagacgggc accgccgaac gctttgcgcg gcagattaac
    18481 gaggcgctcg cccactactc ctatctgccg ctcgttttca tctcggcctt gaccggacag
    18541 cgggtgggca aagtgctggc gctggtcgac caggtgcacg cggagaatac gcggcagctg
    18601 cccacgcccg aactcaacga ctggctcgcc gaggtgacgg cgcggcgcca tcccccggcg
    18661 cggcagggca aacacatcaa gttcaattac atcacccaga ccggcgcggc cccgccctcc
    18721 tttgtcatct tcgccaatta ccccaagctc gtcgacaggt cgtacgtgaa ttacctgcac
    18781 aaccaactgc gggagcgctg gggatttgcg ggcgtgtcgc tccggctgaa attccgccgc
    18841 aagtagcaac cgccccgcgc cgattggcac ccgacgctgt cgcacagccc cctgagtcaa
    18901 ttcacgcccg gccgctgccg ataatcccgg tatggaaacc cggccggtgt acgatgtcga
    18961 cccggagcag ctggcggcct cgctggcggc ccggatcgcc gaccttcagg atctcgccac
    19021 catgggcgcg gtgatcgcgt ccattcacga catcgacgcc atcctctcgg tcgtcatgga
    19081 catgtcgctg cggctggtcg atggcgaggt gggcgcgatc ctgctggaga aaagcgggca
    19141 gttgggtatc gaaatctcgt ggggcgtcca ggaggggctc atccccaaac tcccgggcgg
    19201 cgagacggcc gaagaactgc tgcccgccgg ggaaaccgcc cctcccggcg gtatggacct
    19261 gcccacccgc gtgctggcct ccgggcagac ggtgatcctc aacgacctca gccttcgcga
    19321 tgaagacggg cggcgcatcg acgccgtcat ctgcatgccc atcgggacca gggaccggcg
    19381 ctacggcgtc ctgctgattc tcaaccgggc cggcggcggt ggcttcaacg acgaggatcg
    19441 ggagcgcctg gccatgctcc tcaatttcgt ggccgtggca atcgacaacg cccgcctcat
    19501 gcaggagcag ctcagccgtc agcggatcga acaggagatg gcgatcgcac gccaggtcca
    19561 ggaaaccatc ctcccgcagg acatcgatgc ggtcggcggc gtggaaatcg gcgcctccta
    19621 ctaccccgcg caggaagtgg gcggcgactt ctacgacgtc cacgcgctgt cggataacga
    19681 gttcctcgtc ttcatcggcg acgtctccaa caagggggtg ccggcggcgc tggtgatgtc
    19741 ggcggctgcg ggaatcatca aatccatcct cgaccgcagc cccgagatcg cggtcaatga
    19801 actcgcctcc cgcctgaacg agattctcgc acgcgagatc atccgcgacc gcgagatgtt
    19861 tatcaccctc ttcttcgccc gcttcgatct cgcccggggc cggatgacct actgcaacgg
    19921 cggccacgtc ccggggatgt tctggtcggc ccgggagggg acggtggcca ggctgtcgac
    19981 cggcggcccg attgtcggcc agtttccggg agcgcacttt gatctcggtt cgtgcccgat
    20041 cggttcgggc gaccgcctgt ttttgtgcac cgatgggctc accgaggccg tcgacaaaga
    20101 cggacggata ttcggacggg aacaggcgga agaagtgttc gcgatggaga tcggccttcc
    20161 cccaaaagag ttctgtgttc gcatacggga gtggattgac cggttcgcgt caggcgcgcc
    20221 ggaagattcg cacgacgatt tcaccgtttt gcaggtgctg gtgaagtgat ggcgacacat
    20281 cggtttgcct acccctccgt ggttgaatcc gaggagcggt ttctcaacga tctggcgcag
    20341 gtggcacaac gggagaggct tgaggaggac gtgcgacggg cgatgatgtt gaccttgtcc
    20401 gaggccttca ccaacgctct ggtccacggc aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nntcgccgcg cggccccaaa ccggtcagct cgacgaccac gggcggggga
    20581 tcgggctgat ggagcacttt gccgcggaag tggtcttcgg cgagaccctt accggcggcc
    20641 ttaaagtatc ggtccgattc cgccgactag tagagagaac aaggggatga ggagaagcgg
    20701 cggccggttc cggacccgcg ggaggccccg gggtacggcg atccggctgc aagaaaccat
    20761 tcgtgcggga tgtactggag gcgcgatgac tataacagtc gaaactttcg gaaatgtggc
    20821 ggtgctccga ctgaacgggc gcctggatct ggccagcggc gccgagctca agcagaaggc
    20881 gaaagacctc ttcgcccagg accgcaccac gctgcacctg aacctggccg gcgtcgagtt
    20941 catcaacagc tccgggctgg gcgccctcgt gtcgaccatg aaggaaaccc ggctgcggaa
    21001 gggacggctc acgctctcca acctggcgag ctacgtgcag gagatcttcg aaatcaccca
    21061 gctgtcccat atcttcgaga tctttcccac tgaagaagaa gcgctggtgt ccaaccgggc
    21121 cgccgtcatt gactgatcga cacaggagac cgccaatgcc gcaaccatcg attctcattg
    21181 tcgacgacga actgctcatc cgggatctgc tgtacgactt cttcaccggg cagggatggg
    21241 acaccgcgat cgccgacagc ggtgagaagg ctctggaaat cctgcaacgc cggtcggtcg
    21301 atctggtgct ggccgacatc cgcatgccgg ggatcgacgg cctggaactg accgccgagc
    21361 tccgggaact gcggccgggg ctccccgtgg tcctcatgac cgggtatccc tcggtcgaca
    21421 cggcggtggc ggcgcttcgg catcgggtgg ccgattatgt gaccaagccg ttcaacatca
    21481 ataacctgta caaaatagtc gaggcccagg tggcggcgaa agaagccgcc gccaaaagcg
    21541 agtaggacgg ccatgaaaag cgcaacacgg gagccggtgc tggtggtcga gggggacgaa
    21601 agcatgcgcc ggcggctctg cgactacctc ggccaactcg gctacgcggt ggacggcgcg
    21661 gggagcctcg gcgaggctat ccaaatggcc ctgacccggc cctatgcggc tgccgttctc
    21721 gatgtaacaa tcgggccagg gaccggcgaa gcggccgttt ccgaactgca gttgaaccag
    21781 cccaagatgc ctgtgatcgt catgagcggc gcaccgtcgg tccgggccgt gatcgccgcc
    21841 ctgcggcagg gtgcattcga ctacatcatc aaaccctacg aaatccagga cttggcgact
    21901 atcctgcctc gggcggtcga acggggacgc caggaactgg cggccctggc gctcaccggc
    21961 ggagccgcac cgccacggtg acgactcata agcagccgcg atcaaaccgt gcgcccctcg
    22021 ccgcaacggc cttttccccg ccgatcgttc cggtctcttt cctcccggaa gtttttagtt
    22081 gaatgctttt cggagccgcc ctaaggtagg gtggttcaga cacgagcatg gagtgttggg
    22141 tggccggcgg gcctgcccgg agacgccgtg gaagaaacga tcatggatga cagcgttagg
    22201 tctatcgccg aactcgaagg cgggcaggaa gatacggcgg ccgggggaga ggtgggacgg
    22261 ttcgccgact cctacgcgtc cttcacccgc atcatcaact ccctccagcg ccggtatatc
    22321 cagcttgagg acgacttcac gacccagcac cgcgagctgg cggaggccaa ccgccgtctg
    22381 accgagctga ccgagcggaa cctcgcggtc accgaatttc tcaacggcat tctgaactcc
    22441 atcaacgccg gcgtcatcgc ggtcgaccgc cggggcgtaa tcacgcactt caacagcgcc
    22501 gcggcgcgca tcctggggac agcggcgccc gaggctctgg ggagacagta ccgggactgc
    22561 gtccgccccg gcacgccccc cgacgccaac gccctccgtt cggtggagcg ggacgaggcg
    22621 atcgactcgg tggagcgggt gatcgagcag ccggacggca cccggctgta cctgtcggtg
    22681 tcgaccacca ttctccgcga cgccgccaac cagtcggcgg gggcggtgga ggtgctccac
    22741 gacctgacca agatcaagcg catggagcag gagctggccc ggctgaacac cctggccgcg
    22801 ctcggcgaga tggcggccac gatcgcccac gaagtccgca atccgctctc gggcatcggc
    22861 gggttcgccg ccctcctgca gaaagatctc gatcccgaag atcccaggta ccggctggtc
    22921 ggcaacatcc tcaagggagt cggcaatctg aacgagacgg tgaccaccct gctgaactac
    22981 acgcgtttcg aggagaccaa tcggacggag atcgcctact atgatttcct ccaggggacg
    23041 gtgcggcagt tcgagcgcga ctgcagcgag cggctcgccg gcgcgcgcat tgcgctgcgt
    23101 cccccggctg aaggggtgcg gaacccgcgg attgaagtcg acccgatgct cctgcgccag
    23161 gcgtacttca acctcttcac caatgcggcc gaagcgatgg ccggcggcgg cgaaatcgag
    23221 gtcggctacc gcgtgtacgg ccgggaggag gccgccgcgg tctggggaaa acggctccta
    23281 ctgggtcttg atgagaccgt gctggagaca acggtggccg accaggggac gggcatagcc
    23341 ccggaaaacc tcgagaagat cttctcgccg ttcttcacca cccggggcga cggcaacggg
    23401 ctgggattgg cggtagtgtg gaaagtgatg aaggcccacg ggggcgaggt gctggcggag
    23461 aaccgcccgg cgggcggggc gctcttccgg ctcctcctgc ctgtccggcg ggccggagga
    23521 cagggagtgc gcagatgaac tacgcggtgt tggtcgtcga cgatgaccga ctggtcaatg
    23581 aattcctcgt tgaaacgctc cgaagggcgg gctatgactg cacggcggcc cattccggcg
    23641 aggaagccct ggcccgcctc aacgagcggg atttcgacat cgtcgtcacc gatctgaaaa
    23701 tgcccggcat ggacggcctc gcgctgctgg cccaggtgaa aaaggcgagc ccccagacga
    23761 tcgtcatcat gatgactgcc tacggcacga tcgacacggc ggtgaaagcc atcaaaaacg
    23821 gggcctatga tttcctcctc aagccggttt cccccgagac cgtcgagcac atcatccgcc
    23881 gggtcgccga ggtgatcgcg ctccggcgcg agaatgagct catgcgcaaa gacctggccg
    23941 acaagttcca caacatcgtc ggcaagtcca aagtcatgaa ggaagtgttc gacctcatcc
    24001 agggcatcgc cgacgcccgc tcgaccgtgc tcatcaccgg ggcttccggc acgggcaagg
    24061 agctggtggc gcgggccatt cactacagct cgtgccgggc cgacaaaccg ttcgtgaaac
    24121 tcaactgcgc ggcgctgccc gaaaacctcg tcgagtcgga gctcttcggc tacgagaagg
    24181 gggcgttcac cgacgccaag aagacgaccc gggggcggtt cgaactggcc gacggcggga
    24241 cgctcctgct ggacgaaatc tccgaaatgc cgctcaactt gcagtccaag ctgctccgcg
    24301 tgatccagga gcgggagttc gaacgggtgg gatcgagcca gaccatccag gtcgatgtcc
    24361 ggctggtcgc cacctcgaac cggaatctcc gcgagtacat cgccgccgga cggttccgcg
    24421 aggatctcta ctaccgcctg aatgtcatcc cgatccacct ggccccgctg gaggagcgca
    24481 aggaagacgt cccgctactg gtcgagcact tcatcgccaa gtacaaccgc gagaacagcc
    24541 ggaccgtgaa aggggtcgac gctcaggcga tgcggctgct gatgcactac cattggccgg
    24601 gcaacgtgcg ggagctggaa aactatatcg agcgggcggt cgtgaccggg aacaaggaaa
    24661 tcctcaccga ggacgagttc cccgccgagc tggctctcgg tcgggcggcc gacagcctgg
    24721 cggctctgcg ggtgccgatg aaactggagg aggggaacaa gtacctgatc ctcaagacgc
    24781 tcgagaagtt caacggcaac aagacccggg ccgccgaggc gctggggatc tcggcccgga
    24841 ccatccgcaa caagctggcc gagtacggcg aagaagtgtc caattcctga gcagtttctg
    24901 caagcgggta cggggcgggc agttgccgcc ccgcccgccc tccactaatc atcccccctg
    24961 ccgatagtat tggggtagga tgtgcgggac gcccggcgca cagacgtcgg acggccgaaa
    25021 acgccggcgc cccgccccac ccgtcacggc cgcgggccgg gacggtgacc ccggtgcggt
    25081 cgtaaccgag ttgacatacc catggatctg gcgacactga taggtttggt ggtggcgttc
    25141 ggcgcggtcg gcgggtcgtt cctcatggag ggaggatcct tcgacgccgt cttcctcgcc
    25201 gcgcccatcc tgatcgtggt cggcggcacg ctcggggcga ccacggtgac cacctcgatc
    25261 gacaccgtca accacattcc ccactacctg cgcatcgcct tcttcggccg gtcgcaccgg
    25321 ttcagcgaca cgatcgagca gatcgtgaaa ctgggggaga aggcccggcg ggaaggcatt
    25381 ctcggcctcg agcgcgacat ggagcgcatc cggaacccgt ttttccggaa agccgtgcgc
    25441 ctggtcatcg acgggacgga aacgagcgcg ctccgcgaaa tcctcgaaac cgagatcgcc
    25501 tacctcgagg cgcggcacaa gcgcggcatc actttcttcc agaaagcggg cggctactcg
    25561 ccgaccatgg ggatcctcgg gaccgtcctc ggcctggtgc acacgctggg gaacacctcg
    25621 gacgcctcgc gcatggcggc ccatatcgcg gccgcgttca tcgccaccct ctggggagtc
    25681 ggactggcca acctgttctt tctcccggtc tcggacaaac tgcgcatgcg ccacgaggag
    25741 gagatcgccc acctcgaact gatcatggag gggatcatgg ccctccagtc cggtgagaac
    25801 ccgcggcaca tccgcacccg cctgctcgct ttcatcgccc cgatccaccg caaccacgag
    25861 gagtagccat gcgccgcttt cgccgccggg tcgaagacaa ggagaacctc gatcgctggc
    25921 tgctcaccta cagcgacctc atcacgctgc ttctggcgtt cttcgtcgtc atgtactcca
    25981 tgtcgcaggt cgacgccaag cgcttcgggc agatggccaa ggctctccac ggcattctta
    26041 aaggagggga gaacgtcttc gactacgacg aggcgaaacc gaccgaggac ggccatggcc
    26101 tgctgaaact cggcaacctg cgcatgctgc aggcccgcgt ggaggaacgc ttcaaaggaa
    26161 tcaaccggct ggacgacgtc agctcggaga tcaccgagcg cgggctggtg attcacattg
    26221 tcgaccgggc gctgttcgac gagggggcgg ccgacctgaa gccgcaggcg atcgacgtcc
    26281 tcgatctcat caacgacgaa gtccgcgcta tgcccaacca cctccggatc gaggggcaca
    26341 ccgacgaccg ccagatcgcc acgctgcggt ttccctccaa ctgggagctc tcggcggccc
    26401 gcgcgaccga ggtcgtgcgc tttttcgtga accaacacga tttcccgccc gaccgcattt
    26461 ccgcgctcgg ctacggggag ttccggccga tccgccccaa caactcgatc gagaaccggg
    26521 ccatgaatcg gcgcgtcgac atcgtgatcc tcaccatgga actctcgctc aaagaaccga
    26581 cctcgcagtt gtatttcacg gccgccaacg agtagtcttt tcccggctcc tccggaaata
    26641 ctttccctga cactcgggct cgccccgcgc tgtgggaccg cgcgcgtcag tccaacccgc
    26701 tgggccgcaa ccgcctgcgg cgtccgaccg cgattcggca cgcacgttgc atagacgcag
    26761 cgcgagaaaa accatgaggc cgtgtgtgac cgtatgaaaa tagcggactt tctcttcaag
    26821 tcgaccggcg tccacagatt cgaggcgtac ctggatgtct cctcgttccg gcacaagctg
    26881 atcagcagca acgtggccaa cgtgtcgacg cccgggtacg gcgcccggga catcgacttt
    26941 caagccgagt acacgcgcct caccggcgag accggccggg tggccggcga gttgacccac
    27001 cccgcgcacc tgcctctcgg cgcgcaccgc gaccgcgaac cggatatcga ggagaccaga
    27061 cccaaaccgg atgagctcaa cgccgtcgac atcgaccggg aggtctcgca gatggcccag
    27121 aacgaacttc agtttacggt ggccgctcgg ctcctgcaga agaaatttga ggggctgcgg
    27181 cgggccatag cggggagata ggagagtaag gtatggcggg catactcgga gccattgaga
    27241 ttgcgtcgcg cggactgtcg gtccagcggg caaaaatgaa cgccacggcc gaaaacatcg
    27301 ccaacgccga gaccacgcag accccggagg gcggccctta ccggcgcaag cgcgtgcagg
    27361 tgcgggaaga cgagacgggg gtgtcgttcc ggcaggccat gcggcgcgcc ggcgtctccc
    27421 tcgagcgcac caacacccgg catttccccg cccgcggcgt gtgtgtgcaa tctgaggtgg
    27481 aggtccccga cgtcgacatg aaggaaatcc gggatccgca atcgagctac cggctcatcc
    27541 acgatcccga gcatcccgac gccgacgagc aggggtacgt gaagatgccg gacattgagg
    27601 tggtcaacga gatggtcgac atgatggcgg cggcgcgcgc ctacgaggcc aacacggtga
    27661 cgatcgccgc cgccaagcag atggccaaag atgcactgga catataggaa agtgacaggc
    27721 agccatgaag attaacccga cagcaattca gtcgtaccag cagctcgtgc agcggcagcc
    27781 gcagcgcccg gacgagcagg cccggccggc cgacgccgca cgcgagctga ccatcgcgcc
    27841 gcaggcggcc gcgtccggat ccgacctcgc ggtcaaagcg cccgagggat cctacgccga
    27901 tttcctcacc gaggccgagc gcgcggccct ggagctgctc ttccagcgct tcggcgactc
    27961 cggccgcttc ggagcggccc tgggcggcga ggccgccgag tcccgggtgc tcggtaaact
    28021 gatcgacgtg aaggtgtaac atgacggcac ctgtcaatgg cctcacccgg ttggtgccgg
    28081 gactcgtggc gcggctcgag ccgactggaa agaccgtgcc gttgccggag atgaaggccg
    28141 aggacggttt cggtgagatg ctctccgact ttgtcagcgg cgtcaactcg ctgcagtacg
    28201 aatcggcgca gatccagcag gcgtttctcg aaggcgagcc ggtcgaactg caccaggtga
    28261 tggtgaaagc ggaggaagcc ggggtcgccg ccgagctcct cattgaaatc cgcaacaagc
    28321 tggtcgaggg ataccagcag cttatacaga tgcccttgta gtcggcagcc aacgtgggtg
    28381 cgcggccagg gaccggccgc ggacgtgaca taggatgatg ccatggagca agtgagacag
    28441 tttttccgca acctcgccgc gtgggtcggc cggctctcgc ccggccaggt catgatgctg
    28501 ctgggaatcg ccgccggcac catcgtcggc acgatcctcc tcgtcggctg ggtcaatacc
    28561 gtcacctacg cccgcctcta ttctgacgtc gatgaggccg aggccggcga ggtggtcgcc
    28621 tacctgaacg acaacaagat cccctacgag ttgagcgacg gcgggcggac gatcgcggta
    28681 ccctccgacc aggtctacaa gacgcggatt gcgctggcct ccgagggcct tccccgcaac
    28741 ggcaacatcg gctactcgat cttcgacaag aacaacctcg ggatgaccga cttcctgcag
    28801 aacctcaatt tccgccgcgc cctcgaaggg gagttgacgc gcacgatcat gcagttgtcg
    28861 gaggtgcagg ccgcccgggt ccacatcgtg atgccttccg accgcctctt caagcaggac
    28921 cagaaacagg ccaccgcttc ggtggtcctc aaactgcgca gcggcggcct gaccaaacag
    28981 cagatcaacg ggatctccca cctcgtcgcg gcctcggtgg aaggcctgcg gcccgaaaat
    29041 atctcgattg tcgactacga cggcaacctg ctctcctcgg gccagccgac cgatgagctg
    29101 gccggactga ccgcctcgca gctcgacgtg cgcaaacagg ccgagaccta tctcgaacac
    29161 aaggcgcagt cgatgctgga cgacgtgctc gggccgggac gctccgtgat ccgggtcacc
    29221 gccgaacttg acttcaacca gaccgagcgg acctcggaga cgtacgacgc caaccagccg
    29281 gccgtgcgca gcgaagaacg caccaagcag accaacgcct cgaccgacaa ggccgccgag
    29341 gccagcgaga gccaggaaca gggatccacc gaaacgacca tcaccaacta cgaactcaac
    29401 aagacggtcg agcacatcat caacgggatc ggctcgatca aacgcctctc ggtggcggtg
    29461 atggtggacg gcgtctatac cccggcggcc gccgaggacg gcggcgcggc gatgtcctac
    29521 cagccccgct cccaggagga actggaccgg ctggccgcga tcgtccgcaa cgccgtcggc
    29581 ttcgactccc agcgcaacga ccagatcgaa atggtcaaca tcgccttcga ccgccgcgac
    29641 ctgatcgagg accagcaggc tctcgacagc atgtaccggc gggatttcta cctcgaaatc
    29701 gccaagaaag tcggccttgt cctgctcgcc ctcctggtct tcctgtacct gcggcgcaag
    29761 gtgagccggc tgttccagtc gatcggggcc atggccgcgg cgccgaaacg gacccccgcc
    29821 cggccgctga cgccgggcgc ggcccccgaa gccgccgcgg aggaagggcc gcgcgtgatc
    29881 cccgagcagc ggcgaacccg cctggtcgat gagatgcagg acgcggcccg caagcagccc
    29941 gaagagatcg cccgcgcgat caaaaccatg atgagtgacg tctgatggga gcgctcaagt
    30001 acgaatcgat gacgcccctg cagaaggcgg cagtcgccct ggtagccttc ggaccggaag
    30061 tgtcggccca cgtcctcaag gggatgcccg aggccgacct cgagaagatc accattgaaa
    30121 tcgccaatct gcgggaagtg ccgcccgatg tcgaggacaa ggtgatcgac gagtgctacc
    30181 agattttcat ggcgcgccag tacatctcac aggggggaat tgatttcgcc cgcaagatcc
    30241 tcgaatcggc ggtcggcgtc gccaaagcgc gcgaaatcat gcagcgcctc gaaaccacca
    30301 tcaaatcctc cggcttctcg ctcctgaaag acatcgaccc gaaacagttg accagttttc
    30361 tccagaatga acacccccag accatctccc tgatcctcac ccagctctcg ccccagcacg
    30421 ccgcggccgt gctctccgag ctctcgccgg aattgcaggg ggaggtggcc ctgcgcatcg
    30481 ccatgatgga gaaaatcgcg ccggagatcc tccacgagat cgagcagacc ctctcggggc
    30541 atttcgacgc cggcgccgcg gcccagatgt ccgcgtccgg cggcgccaaa gccatcgccg
    30601 aaatcctcaa cctgatcgac acgacggcgg agaagaacat cctccagtcg ctcgaggccg
    30661 acgaccccga cctcgccgcc gaagtcaaga acatgatgtt cgtcttcgac gatctcacgc
    30721 tgctcgacga ccggtcgatc cagcggctgc tgaaggaagt ggagaccaag gacctcgcga
    30781 tcgccctcaa agcggccagc gatgaagtca agaacaagat cttcagcaac gtctccgaac
    30841 gcgtcgccgt gatgatccgg gaagagatgg agttcatggg accgacccgc ctgtccgatg
    30901 tcgaggcggc ccagggacgg atcgtcgagg ccgtgcgccg tctcgaggag gaaggccaga
    30961 tcatcgtctc cggccggggc ggcaaggaag atatcattgt ctaaggtact gcgacacgtg
    31021 cggccggcgc cgacggtgtg cgtgggcgaa cggcaactcg accatgaccg ccaatcccgc
    31081 gccgtcgccg tcctcggccg gctcttcccg gcggtggcga tcgtcaccga ccccggcgga
    31141 gcgcgcttca tcccggtcga agaagctttc acaatggagg acgcctgccg ggcggcgcac
    31201 gaggaggaat accggcgcgg ctatcgggag ggccagcagg ccgggctcgc ccagggattg
    31261 gcgaaagccg aacaggtgct cagccggttc gacgccgcga ttgcggacgc aatcgaacag
    31321 cggcaccggc tgctcgacga ggcgcggcgg gagatcctcg acctggtcgt gcagatcagc
    31381 cgcaaagtga ccttcgacgc ggtcacgatc gatccggagt cgaccctgcg gctgatcaac
    31441 ggcgtgatcg acaccctgct cgaccgctcg cgcatcagga tcaaggtcca ccccggccac
    31501 ctccccatcg tggaacagag catcgcgcag tttctcggag gctcggccct gatcaaggaa
    31561 ctcacgatcg agcccgaccc ccgcgttcgc gaagggggat gtttgattga aacgccgcac
    31621 ggcgatgtcg acgcccggct ggaatcgcag ttcgaagtcg tgcgcgaggt tctcctcgcc
    31681 gaagaggccg gccagtgagc gctccctcct atcatatgta cgccgagcgg attcgggccg
    31741 cccacaccat ccgacactac ggcaaagtga tgcaggtggt cggtctcgtg attgagtcgg
    31801 ccgggccggc ggtctcgatc ggaaagctct gcaccatcga aaaccacgac accggagcca
    31861 agatccgggc ggaagtagtg ggttttcggg acaaccggat cctgctgatg ccgctcgggc
    31921 cgatcacggg catcacgccc ggggcgatcg tcacctcgac cgccgagcac ctgcgggtgc
    31981 cggtcggccg cgaactgatc gggcgcgtgc tcggcggcct ggggcagccg atcgacggca
    32041 agggtccgct cggcacgggc gcgtggcggc aactcagcgg cgaaccgatc ccggcgctga
    32101 aacggcggcg gatcaccgaa ccgctccgca ccggaatccg ggccgtcgac ctcgtgaccg
    32161 ctgtcggaaa gggacagcgc ctggggattt tcgccggctc cggcgtgggc aaatcaatca
    32221 tgctcgggat gatggcgcgg gggacgagcg ccgatgtcaa cgtcctcgcc ctcgtcggcg
    32281 aacgcggccg cgaagtgcgc gagtttgtcg aatccgacct cggcccggag gggatgcggc
    32341 gctcggtcgt ggtggccgtc acctccgatc aaccggctct catccgcatc aagggggctc
    32401 tggtggcgac cgccattgcc gaacacttcc gcgatcaggg gatggacgtt ctcctcttga
    32461 tggactcgct cacccgtatc gcgatggcgc agcgggagat cggcatcgcg atcggcgagc
    32521 cgcccactgc caaaggatac acgccctcgg tcttcgccct cctgccgggc ctgctcgagc
    32581 gggccggcca ggccgagaag ggatcgatca ccggcctgta cagcgtgctg gtcgagggcg
    32641 acgacttcaa cgagccggtt tccgacgccg tgcggtcgat cctcgacggg cacgtggctc
    32701 tgtcgcgccg cctggccgcc cgcaaccagt accccgccgt cgacatcctc gattccgtca
    32761 gccggctcat gaaatcggtc tgcacgcccg agcaggtcga actcgccggc caggtgcggg
    32821 agctcctctc ggtgtaccgc gaggccgagg acctcatcaa catcggggcc tacgtgaggg
    32881 gttccaatcc caagatcgat cgggccatcg ccaagatgga tgcgctcgac gcgttcttcc
    32941 ggcagggtat tcacgaaatc tcggacttcg accaatcggt cgcccagctc cggcagctcc
    33001 tcgccgactg aggtcccgga ggcgccgcgt gaagaagttt cgttttcgcc tggaagcgct
    33061 cctcaagcag cgggaacaga ttgagaagga ccgccggcgc gaactcgccg aggcgatgcg
    33121 ccggacggcg cggcagaaac aggcgctcga cggcctcatc gagcgcagcc gggaaacgct
    33181 cgcacacaag cgcgcgcggc agacgggcac cctggccgtc gccgaactgc agttatgcgc
    33241 gcgcttcctc catcggctca aacgcgagac gctgggggcg gacgaggtcc tccgcgccct
    33301 ccgcaagacc gaggccaaga aacgcgaggc gcttctcgag gccgccaaga accgcaagat
    33361 ctacgacacg ctcaaggatc accgacgcag cgaattctac cacgaggccg aacaacgcga
    33421 gcggaaagag accgacgaaa tcgggctgaa cgtctttcgg gcgggccgcg gaaaaccggc
    33481 ctaaacaaac agcccggcgg cgccgggctg tcttcagaat cattttgaat ggcctgcgac
    33541 catcaactcc ggccgctact ggcacggagc cggcggtgga ccgccccgga agaggtaggc
    33601 gatgaggtac gtgatgtcgg ctaccgtcgg ctgaggcgtg gcgtcgccat tgatgttggc
    33661 ttcttccgga caatcagccg gggccatgcc gcggaacaaa atcccgatca agcgggtgag
    33721 atccggcagc gtcacggcgt cggtcggatc gccatcgaca tttccccggg tcccgatgca
    33781 gcagccgaac ccctggtcaa tcttcaccca gccgcgccgg tatttcggcg tgaaggccac
    33841 ggccgacgcg gtcgagaacc tggtcgaata ggagacgtcg acattcacaa tcgtgatggt
    33901 gtcgatgctc accagctgcg ggctgattcc cacgctccac gagagataga gccgcccgag
    33961 gaggccggaa gcggccggga tcgtgggttc tcccgcgaac ggcacgcagt agatcgagaa
    34021 gcccccggaa ggcgtgtagg tccccttgac cgaatacgat tgcagccggc cgcccgcgaa
    34081 cgagaaggag tcgacatgga catcgggaga actgaagtgc agcgtcacct ccagcccgcc
    34141 cagcggctcg tcgttgtaga agttgaccgg aacgatggcg cgagtgttgg tgaaagcgcc
    34201 gatcgagtca acgtgcaccg tgtccggaat accgggatcc tgcgccgccg cggcgggcgc
    34261 caggagaacc atcccggcaa gacacaacag gcaagctcgt gcaatccgac tctgcatacg
    34321 gataaccctt tctttcacga ctgaactgag caaattcaat gcgcgttatc gaagaaaggt
    34381 cgcttggcct accaggttgc ccggccggaa ccggggggtg aatgtaagta actatgagcg
    34441 aagctctttc cggaaagtaa tatagggaca atcgaccggc ggcgcaaccg gaaagatcag
    34501 gcgtccgcca accgcggccg ggcgggttgc acaagcggtg ctgcaaccct tttcccaccc
    34561 cggaatacgg ggagcgtctc acaaacctca ccccgcccgc cgatacaggg gatatgccgg
    34621 aggaaatgac ccccaggaca gtattccggt gtacttacat ccgggcctgc tacctcgcgg
    34681 atgagcttaa gtgcttcggg tacaagaccg attgcccgct gtaccggaag tgcaacggcg
    34741 aatactccga cgaggcggca ttccacgagg cgatggaccg cctgatcgac aggacccggg
    34801 cggtctacgc cggtctggac aagtaagacc cttccccgtg accggggaga gcgccgctgc
    34861 caggatctgt cctgacgaat tcggggagcc ccgcgccccc ggtcggcgcg caatccctct
    34921 tgcggatttg ccgaaatctg cctatccttg ccccatactg catggcgccc ccgccctggc
    34981 ggcggtggag cgcgggttca ttagggtcct gccgcgatct acgactcgcg taaacaagca
    35041 gatgcgtggt gtgcagtcga cctatgtgtg gaggctcgga atgtttcgtt taattgcggt
    35101 cgtcatggtg agcgcactcg tcgcggccgc cgccccggcg gccggcacgc ccggaagagg
    35161 gagcgtgccg actgtcaccg tcttgccggg ggcgggcgac accctgcccg tgacgcccac
    35221 ctacgagtgg attgacatat actgcggcga gcccacgctc gacggcgcgc cgctggcgcc
    35281 gggcgacacg atcttcgtct acgacccgga caacatgtgc tgcggcgcgg caattgtcca
    35341 tgccgacggc atgtacgggt tcgctcccgt ctaccgggat gacgcctatt cggccgagga
    35401 tgaaggcgcg gaaccggggg atacgctgcg ctttgtggtc aacggcgtgc ccgttgcggc
    35461 tgcgcccccc gtgatctgga cggcgaacgg ggagagcttc gccgtctgcc gattcctcac
    35521 ggccccgccg gcataccggg aggtttccct cgacatcaaa cccgggtcgt gtccgaaccc
    35581 gctcaatctc gtcggaccgt cggacaacgg caaggccgtg ctgccggccg ccgttctcgg
    35641 cgcgccggat ttcgacgtcc gcacgatcaa cccgccgacg ctcgcgctgg gcggcgccga
    35701 agttgtccgc tggagttacg aagatgtcgg ccggccgaaa gccgatccat acgacacctg
    35761 cgcgtgccac gagtacggcc ccgacgggta catggatctg accctcaagt tctatcgcgc
    35821 agaggtcgcc ccggccgtct cgtttgccgc cgatggcgat tacgcaccgc tcgtcctgac
    35881 cggatcgctg cttgacggca ctcctcttag cggcgtcgat tgtgtgcgcg tgcagcgcgg
    35941 cgggaggccg gagggcaata gcgggggcgg cccgaccgga aaggtgcagg ccccggccga
    36001 tttcgcactc gccaaccatc ccaatccctt caacccgtca acccagatca ccttcgccct
    36061 tccccaggcc gggccggtca ccctgcgcgt gtacgatgtc caggggcggc tcgtgcggac
    36121 gctggccaac gggcctttcg atgccggcgt tcactcggtc gagtggaacg gaaccgacgg
    36181 caccggcagt gccgtggcca gcggcgtgta cctctaccgg ttgtcggcgg gggagacggt
    36241 cgtgacccga aagatgatct tgatgaagta ggtcgggtcg gcagggacaa agtgaagggc
    36301 gcggtccggc agggccgcgc ttttttttgc gggcggggcg gccaggcggc gcgccgcgcc
    36361 cgagggcgga ggcggttagc cctcctccag gcggcgcagc ttcttgtaca gcagggagcg
    36421 ttcgatgccg agctcgcggg cggcctcggt catgttgccg ccgtggcgcg cgattgcggc
    36481 ctcgagatac tcgcgctcga actccgccat cgcctctttc agcgaccggc gaccgccgcg
    36541 cgcggccgcc ggcaggagcg gcttcaggtc ggccacagtc acgacactcc gctcacagta
    36601 gatgttgacc cgctccatca ggttcttgag ttctctgatg ttgcctggga aggagtaagc
    36661 gcacagcagc ttcaggctgt cgcgctcgag acgtttcggg accgagccgg tttcgccggc
    36721 aaagcgggcc aggaagtgat cggcgagcaa agggatgtcc tgggtccgct cccgcagggg
    36781 ggggaggtgg aaagtcacga cgttcaggcg atagagcaaa tcctgccgca gcgagccgtc
    36841 ggccgccatc gtctccaggt cgcggttgga ggcggcgatg atcgtgcagt cgaccgagta
    36901 cgttctgctt gccccgaccg gggtcacttc gcgcgtctcg atgacgcgga gaattttcgc
    36961 ctgggcgccg gccggcattt cgctgatctc gtcgaggaaa atcgacccct ggtcggcctc
    37021 gacgaatctt ccccggcggt cggcgcgggc gccggtgaaa gcgccccgca catgcccgaa
    37081 aagctcggac tcgaccaatt ccgatggcag agcggcgcag ttgacggcga caaacggtcc
    37141 gttgctccgg ccgctggcgc gatgaatcat gttggcgaca agctccttgc cggtgccgtt
    37201 ctcgccgagg atgagaaagc gcgccgcttt cggggccgcc cgcaaaatgt cggacttcag
    37261 tttgagtatc tccggcgact cgccgacgaa ctccccgtag gcgcgcaggg cgaggcggtc
    37321 ggcctcggcc tgtagccgcc gggtccgcac cgcattgtcg accgtgacga gcacgcggtc
    37381 cagagacagg ggtttctcga tgaaatcgta ggccccggcg cgcgtggccc ggaccgccat
    37441 gtcgatgtcg gcgtggccgg agaacatcac gatgatgtgc cggggattct tctcgagcgc
    37501 cgccttcagc aggtcgatgc cgttgccgtc gccgagcagc acgtcgagaa aaatgagatc
    37561 gaaacgtccg gacatgaaag tctcgccgcg cgcgtacgat tcggccgtga caacctcgtg
    37621 gccgcggcgc tcgagcgccg ccttcagcga actgcggatg ctctcctcgt cgtcgacaat
    37681 caggatctta gccatgttcc accggcagtc tgagggtgac cagtgcgccg ccgtcggcgc
    37741 ggttctgcag atcgatcgcg ccgccgtggt cgtggacaat tcgctggcaa atgaccagcc
    37801 cgagcccgct gccgcccttc ttcgttgaaa cccgcggttc cagaccgcgc tcgagcaggt
    37861 cgcgggcgaa accggggccg gtatcggcta ccgtaatcac gagcgccccg ggctcgtccc
    37921 ccagcgcaac cgccacccgg ctctcgggcg aggcttcaag accgttcttc accaggttga
    37981 ccagcacctg gtgcatcatc tccgggtcga ggcggacgct ccgccgcgcg ctcctgttcg
    38041 acaacgtcag acggccagtg cccgtctcgc cgcggtagag cgcggccagc ggttccagga
    38101 gtctcgccgg ggccacatcc cggatgaccg gcggtcccat ccgtgcgaag cccacgaatt
    38161 catcgagcag gcgggtgagg cgctccacct cggacttgat cgtcgccgtc gtctcttcga
    38221 ggatcctggg gaaatccggg agctgttcct ggaacgaacg gcggagatcg tcggtgctga
    38281 tcacgatcgg ggtgaggggg ttcttgacct catgggcgat cttgcggccg acggcctgcc
    38341 aggccgcgat cttctcagtc gtggccagcc gccgctgggc gtctcggagg cggaaggtca
    38401 tggccgtgaa agaatccgcc agttgggcga actccccttc ctcgtaggcc atgacgggcg
    38461 tgctgaggtc accgcccgag acgcgggcgg tcgccgtgag gaggttgtcg aattcccgct
    38521 gggcgcgccc gctgatatac aggccgagcg ccacggccgc cagcaccgat ccgagcgcga
    38581 ccagcccgct cacaaccagg aggttgccaa aaacgcgctg ctcggggccg gaccggaagg
    38641 aagcggcgag gaaccagccg gctgcatcgc cgccggcgag caccgcgacc agcgaatccc
    38701 gttcggcgta gagacggccg gggtccatgc ccgacaaccc gccgccggtg tcgccggcca
    38761 gcacaatgcg cacgtcggct ccgatcaggc gcgccaccgt cccggtgtac tggctgtcga
    38821 gatagcggcc ggcatagacg acataggcgc tttcggccgg aagtctccac gccagcgcgg
    38881 cgtgggggcc gttcaggtca tactccacgg acgccagcgg cgccgagtcc gccgccaggc
    38941 tgtccaggga cgggagcctc tccccgatga gccccgggcg gcgggcgctg gcctgcacga
    39001 gcagggagga atcggcaatt tcgaagaagt ccagccccgc cggtacggcg tcgaaacgga
    39061 catccgatgc ccggccggct ttgagcagaa gcagggcgcg cgcggccgag ggcgtggcgg
    39121 cggcggcgat caccgcctgc cgcaattcat cggccaccgc ccgctggtac tgcgcgaacc
    39181 ggtcaaggct gtgctgcgcc gaccgccgct cggccgccgc agcctggcgc atcccgagca
    39241 ggtagatgac cgacaccacc aggaccgtcg gcaccacggc cacgaggatc aggtacaagc
    39301 ggatgcggcc cgtgaaagtc atggccggca gtccttccgg ttctgcggaa ttcggacgcg
    39361 ggccagaccg gatacgtcga taccgccccg gcggtcgaac acctcgccga gcaggctccg
    39421 gcgggccgtg aaatacaggg tggggcgaaa cagcgggaat accccgaggt cgtcgcgcag
    39481 gacgctctcg gccagttgca cgtaccgcag cgccgccgcc ggctccgccg ccagggccgc
    39541 cgaatcgagg caactgctga cagcctgcag cgactgcgcc gccgccgagc cggccagagt
    39601 atcgagggcg agcaggccgc tcaagcagcg cagaccatcc gcctcgctct caccgagcgg
    39661 aaccgcgacc aggcgaaggt cggctccggc gagcgtggag gtcacggtgg ggcggaaacg
    39721 atcgcgcgac aggatgtccg cgaagtagcc ggcggccggc tcgaacatcg ggtgctccac
    39781 ggcgatgacg accgagcggg agggaccgcg caggttgttc agcagcgcgc ggcccgggcc
    39841 gggcgcgtac gggtagggac gcgcggccgt gtcgccaccc accccgcagc ggttgaacgc
    39901 gcggagctca tccccctcga atatcatcgc cagccgctgc ggatcgagcc gatagtagag
    39961 cgacgtggac agaaaaccac gctgggcaat cgcccgcgcc gggttgggaa cgagggcgac
    40021 gaagaaggga gcgggccgac tgactgaggc gacatcttct ccgcccgccg tgtaccggcg
    40081 gtacgagaag aacccgtcga tcacctgatc ctcgaatgcc agcgaagcct caaaatgcga
    40141 agcatacggc cggaacacga cgcggcctcc ggcggtcgag tcgccgacgc cggccagggg
    40201 cgccgggagc aagccgacct ggccgagcac gatcggggcc agcgtgtcat aaggggcacg
    40261 ctccagccac acgcggagat cggccaggcg gagagtgtcg aagaagccgc cgaccgggga
    40321 gctgaccgcc accccggggt aggaggattc gatgcatccg ctgtagagcg aatcgggacc
    40381 gcgatgcacg acaaaccggc agtcggggaa cacacgcgcc tccagtccgc aatcgaggta
    40441 gaggcggtcc aacttgacct gcagagggcg actctcgatc cacaggctgt cgcccggctg
    40501 cgctgcgcag acggccgcca ggaggagcgg aagcgcgaag atccatgagc aacgagtcac
    40561 ctgagacctc atgccctgta tacacgtgag aaggcctgcc ggatatgtgt gtcacgcatg
    40621 acacgcgata acctgttgaa ttatatcggc aaccgactgc tcgcgcaaca attgtgtcat
    40681 gagagacaca gaccaggggt ggcatcgacc acggggaatt ccacgtatgc tattgattga
    40741 caatatgtta gtcaatttgg catatcgctt gagatgaggg aactcgacat gaaccgaacc
    40801 ctcgaatttc tggaggtagt gatgaaaacg aaaaggattc ttgaaatgat ggcgatagcg
    40861 atggctgtcg cggtgttcac tcttactggc tgctctggcg acggcaaagc ggagaagccg
    40921 aaccaggcgg cgccggcggc tgaggccggt gccatgccct ccaccattgc gggagccgaa
    40981 ctgctcgggg ccagccggcc cgcggtgacc ggggaaaagg ggtttccgat cgccgacatg
    41041 atccgcgtcg attcgaccct ctacgcggtg gccggaaacg agctgattat caccgatctc
    41101 aaaaccaaaa gcgagcggcg ggtgagcgcg gccgaagggc tgtacgccgt gacggagcac
    41161 aacgggaaga tctatgcggg cggaaccgca ctctacgagt tgggggagac cgggctcgaa
    41221 ccggcggcgg cggcgttcga cgggaccatt cgcgccctga ccgggcacga gtaccggctc
    41281 atgatcggga cgagcacggg gctcttcgcg tcttcgattt tcggcgatga ggcgctcttc
    41341 gaaggaatcg acgtgaccgc tatggcgggg gatagcgatg aactgtgggt ggggaccggc
    41401 ggacaggggc tctatcgttg ggatggggcg gagttccagc gccgctatct gcttcgcgac
    41461 acggcggtgt tcgacttcat caacgccctc gccttcgccc acggccacct ctttgtcggc
    41521 accgacgagg cgctctatgt ttacgatggc gggcggtggg agacggtgac gactgacgac
    41581 gggctgccgt cgaatcggat caggtcgatc gacgcctcgg ggtgggtcgt ttacctcggg
    41641 acaccggcgg gagtagcgtc gtggttcaac tacgatgtgg cgccggtggc gaggctcgag
    41701 gaccgcgccg ccacccttgt gcggacattc ggccgcaagc tgatcgtggc gaccgagcgg
    41761 gagggagtgc ttctgaaagc gggcccggtg gtgtcgacgt tgatccgggc ggagacgatc
    41821 ccggtgttgg ccgaggcggc gccgcagttc tccgagccgc tcccggccgg ggcgctggtc
    41881 gattcggcgg acgcagtggg cgcccagccg gtggcggcgg cgaaatgagg cacgacgatc
    41941 ataaccgata gcgcgattct atcgcacccg caggctctcc ggcgccgcgc tcctcccggg
    42001 gggcctgcgg cgttttgggg gaccgttgct ttgttagttt gacgcaggag acggagaccg
    42061 cgccgggata ccctcgcctc cgggaagccg cgggagccga gggactcccg cgcggcgagg
    42121 ctctgtcggg tgtcagcgga ggagccggag gacaatgagc aagatgacgg cgccgatgac
    42181 ggacatgata aagccggcgg gacggaattt ggccgcactc ggggttttgc tgaagcggtc
    42241 gccgatgaga ccgccgagga tgcctccgac aatacccacg gctgtagtgg cgaagaagcc
    42301 catgtggtcg gcgccgggca ggatcgcccg cgcgacgagg ccggccagga agccgacgat
    42361 aatgtaccag agaatgttga gcatggagag ctcctttcag actgcaggat cccaatcgaa
    42421 ccggaggcgc ccgggaggct gacgcgggag aacaggtatt tccgtgtcag cgcaggcgcc
    42481 tagtttcgcg gtcaattacg cagttgtcaa aatggggggc aagcggaatt cgacggtgtc
    42541 ggcagcagac caggtggtgc gggggggatt gatgagctgg aacctgccaa aggaggcatg
    42601 gggaagcata gggcaggggg gcgagcccaa gacggaagcg gcgcctaagg aatgtcatcg
    42661 tcactcggat aatctgtggg ccggttctct ctcacgagag tcgcatgtcc ggttcccttg
    42721 cgtcggggca gaggcagacc ccgcccgtac gcgcgttgga gccagggaaa ggcgtatgca
    42781 tgggcggccg ccaagagccg cgtatcggta agcgccggtc aaatcgaccg ccgtccacca
    42841 gaagtcgggt cgtcctgggc cgcggatgac tgccgcatca agtagcgtct acttcattct
    42901 acttgtccga tttgttccgg gccgacccct ggactttcgc ctcgatcaac ctgcgttgtg
    42961 cctccagctc ggccgcccgc tccgcttcaa ccgcgccccg ggcgtcatat agctccgagt
    43021 gctccggagt gtcagcggac gacccggtgg cctccacact accgtatgcg gttgggaaga
    43081 acatcgccgt caggcggaga ttccgaatct cgaactcgtc ggaagtattg tcggtcatcg
    43141 tggtcaggta gaacttcttt gtcccgctca ccaccggctg cactcgggtg agggagaacg
    43201 gaacgtaatg gtcactggat gcgtagttcg ctctggcctc gaccacgcat cgaccggcgt
    43261 gattgggagc gcgtcgccga aatgcggccc gaggctgcgc tgcgtccgac ctgcgcgttg
    43321 acgtgtcacc gtgctggatc ggcgcccagg gccacccgga tccggtattc ccgcagggag
    43381 agggaaaaat cgtccgtacc ggatctgaat ttctttcccg aaggcagtcc gcccgcggtt
    43441 tcgggggcct ctccaagttg ttactgagca agaccttatg ggcgagagcc ggttgtggca
    43501 cgcctcctgc tttaggaatc cccggagaaa tactatgatc aaaggactct tcagatcagc
    43561 ctccgggatg ctgcccgcca tcaagcggca ggagaccatc gcgaacaacg tcgcgaacgc
    43621 caacaccgcg ggattcaaga aagacgcggt gttcaccaaa gaactctccc gcgcccagcg
    43681 aaaactcgtg cccgtcaaat cggactggga gcagccgatg gtggacgagg tgtacacgaa
    43741 tttcgaaggg ggctcgttca gccggacgga caatccgctc gacctggcga tcgacgggga
    43801 cgggttcttc accgtgcagc tccccgatgg ccgcaccgcg ctcacgcggt cgggcgcgtt
    43861 ttcgatcaac cgcgacgggt tgatggcgct ggccgacggc tcgacgctgc tggccgaagg
    43921 ggggccggtg gaggtcggca acggaaagct caccgtgacc gcggcggggc agattgaggt
    43981 ggacgggagc gcggtcgggc aggtccgacc ggtgaccacg gccgatgtgg gggcgctcga
    44041 gaaaatcggg gcctcgctct tcgccgtccc ggaggggacg gagctgatcc cggtggaaca
    44101 cgctgagatc cgccaggggt acgtcgaaga atcaaacgtg gatatagtcc gcgagatgat
    44161 cgacatgatc gtggccttcc gcgaatacga ggccaacgcg cgggcggtga cgacccagga
    44221 ccagagcctg gacaacctct tccggcgggt gggcggcgat ggataagcgg ccgatcaccc
    44281 cgtttgcgcc ggcggccgga tccggcgaga agattgatgc acgcgtgaga caggagatat
    44341 gagatgatca aagcgatgcg gacggcggcg accggaatgg tcgcccagca gatgaatgtc
    44401 gacaacatcg ccaacaacct ggcgaacgtg aataccacgg gtttcaagca ggcgaaaatc
    44461 gaattccagg acgtgctcta ccagaatctc cggcgggccg ggacggcgac cgcgatcggc
    44521 accgaggtgc ccacggggct ggcgatcggg tacggcacgc gcgcctcggc gaccgtccgg
    44581 cagtacgcgg aggggaacct gaccccgaca ggaaatccgc tcgacctggc gatcgacggc
    44641 gacgggttct tccaggtgca gatgccggac gggaccacgg cctacacgcg cgacggggcc
    44701 ttcaagattt cgggcgacgg ccagatagtc acttccgacg gatacttcct gctgccgcag
    44761 gtgtcgatcc cgcaggatgc gacggcgctg gcggtgggca ccgacgggac gatcgaagtc
    44821 atggtggcgg ggcagagcga tccggtggag atcggccaga tcgaactggc gcggttcgtc
    44881 aacccggccg gactgctcgc ggtcgggcgc aacctgctca cgcccacgcg ggcctcgggg
    44941 gacgccctga ccgacgtgcc gacgcagagc gggctgggcc agatcgacca ggggtatctc
    45001 gagatgtcga acgtgaaagt ggtcgatgaa atggtcaaca tgatcgtcgc ccagcgggcc
    45061 tacgagatga actcgaaggc gattcagacc gccgacgaca tggcgcagat cgcgaacaac
    45121 ctcaagaggt agcggccatg accgagctga tacggaaaat catcctgatc ctgctctttc
    45181 tcgcgttcgc ggctgccggc ggggccgttc cggtcacgca ggcggcgatc gatgaactgg
    45241 tgcgcatgta cgggctcgac acgacgatgt atgcgatcga cgtggacctc aaccggctcc
    45301 acacgacgga ggtcgggacc atccccgcga ccgcgccggg cgcggccgcc gagccgtccc
    45361 gactgaccct gcggccgctc tcggccaaag agccgctcgg gctgtttacg gcggaggcga
    45421 ccgtggagca gggcggggcg ccggtcgaga gcgcgatgat ccgctttcgc atcacgaaat
    45481 tcgcccaggt cctggtggcg gccgaccgca tcgacatgcg cgcgccgatc gccccggagc
    45541 aggtcgaggt gcggcgggtc gacgtgacga cgatgtacga aaagccgctc accgcggtcg
    45601 gcgaactggc cgggatgcgg gcggcccgca tgatccggaa ggggacggtg ctgacgacgg
    45661 cctgcgtgga gatcccgccg gacgtcgaat cggggcgcac ggtggagatc gtgtaccgcg
    45721 acggtcagtg ccggatcgcc acccgcggga cggcgctgca ggcggggcgg gtcggggagg
    45781 ccatcaaggt gagaaacgat tcatcgggca aagtcatcac ggcccgcgtc gccgacgccg
    45841 gcgcggtgac cgtggagcca taggaaggcg gagcgatgaa actgctcaaa gcgctcatca
    45901 tcctgttgat cctgctgctg cttccgtact cgctgaagct caaggggcag gatttcggac
    45961 agagccgttc gctgttcacg gacgtaaagg ccaaccgggt cggggacctg ctcaccgtgc
    46021 tcatctacga gaacaacacg gcgcagcaga acgcccagac caaaaccgag aagagcacgg
    46081 aagcctcgac ctcgggggga ccgggggtcg ggagcctcga cttcatcaag ctgttcgggg
    46141 cggacgcgaa aaacgagagc accttcgacg gcaagggatc gaactcgcgc aacgggagtc
    46201 tccgagcgcg catgtcggtg acggtggtcg aggtgcgcgc caacggcgac ctcgtgatcg
    46261 aaggatcgcg gacgatcggg atcaacggcg agcgtgagac gctccacctc gccggggtgg
    46321 tgcgccagaa ggacgtcacc ccgcagaaca cggtggattc gtacctcatc ggcgacgccg
    46381 agatccacta cacggggaag gggagcctga acagcgggac tcggccgggc attttcaccc
    46441 gaatcatcaa ctggctcttc taggaggcgg gacgtggtgg gaacaatgtg ccatcgactg
    46501 gcgacgccga ccgggctcgt gatcgcgctg gcgattctcc tcgcctgggc gacaactgcg
    46561 gaggcgaaca aggtccgcat caaggatatc gccgacttcc gcaacgaaca gcaggtcgac
    46621 ctgatcggct acggactggt gatcggtctc gacggaacgg gggacgggac cgggacccag
    46681 ttcaccgtgc agtcgctggt caatatgatg cagcggatgg gaatgacggt ggacgtcgag
    46741 aaggtcaagg tcaagaatgt ggcggcggtc atggtcaccg ggcggatcac gagcagccag
    46801 acggagggcg ggctgatcga cgtgaccgtg tcgtcgatcg gggacgcgaa atcgctgcag
    46861 ggggggacgc tgctgatgac cccgctgagc ggacttgacg ggacggtgcg ggcggtggcc
    46921 caggggccgg tgtcgatcgg cgggttcaac attcaggtcg acgaatcgaa caagctctac
    46981 aacaactaca cgctcgtggg gcgggtgcca aacggcggca aggtcaccgc tccgccgacc
    47041 cctgagaacg ccaccgagcg tgaaatctac ctctctctgc gaagccccga ctacaccacc
    47101 gctcaccggg tggcggaacg gatcaacatc aagtacggcc tcctcgccta cccggtcgat
    47161 gccgggtcgg tgcgactggt ggtgcccgac tcgctgtcgc acccgacggc gcggatcgag
    47221 ttcttgtccg acgtcggcca gctccaggtg gtgccggaca acccggcgcg ggtggtcatc
    47281 aacgagcgga cggggaccat tgtggccggg gagcacgtga cgatcgacgc cgtggcgatc
    47341 gcccacgggt cgatcacggt caatattgaa agcacgcccg tgatctcaca gcccgaaccc
    47401 ttctcgcagg gggagacggt ggtgacgagc gacgcgcgga tccaggtcca ggatgagaaa
    47461 gcgcgggtgg tggaactcaa gccggcggtg tatctgtcgg acgtggccaa cgccctcaac
    47521 cggatcgggg cgaccccgcg cgacatcatc gccatcttcc aggcgctaaa gcagtccggg
    47581 gcgctccgcg cggagctggt gattctgtaa tgtcaaccct gcgacccata gacgcgccgg
    47641 tcgtcgcgaa cggcgacctc gatcggctgc gcggccccgc cgctaccccc gagcaggaga
    47701 gagcgcgtct gcgcaaggcg gcccaggaat tcgaggccct cttcatgtac cagatgctca
    47761 aagcgatgcg gcagacggtg ggagagagct ccctggcgaa aaacctcccc ctcggcggaa
    47821 gcctgggcaa ggacacctac actgaccttt ttgatttgga gctcgcgcgc ggcatggcca
    47881 ccggcgggca cgggtcgatc tcggatatcc tctgccgagc gctggaaaaa acagtgaccg
    47941 cggattccgg gcagacgccc gtgccgacgg cgatccggcc gctggagagc ggcgaccggg
    48001 agcgcaaggc gattgccata caggggccgc ggctgccgct ggaccggggg gagccgcacc
    48061 cggcgcccct ggcgccgaaa cggccgccgc cgcttccgcc ccggccgaat cacgcggcgc
    48121 cgcccgctgc cccgccgccg ccgcgcgcgc cggtgcaggc gccgcccgcc gagccgatgc
    48181 ccgaaagcgc cccggccgac gcggccgatc ccatccaggc gcggttcgga cggtggatcg
    48241 acgaggcggc cgaggccaac cggctcgatt ccgcgctcat ccgcgcggtc atcgccgccg
    48301 agtcgggcgg gaatccgcgc gccgtatcgc cggccggcgc gaaaggactg atgcagctca
    48361 tggataccac cgccgcggac atgggtgtga aagagccgtt cgatgcgcgc gagaacatca
    48421 tgtcgggaac caaatacctg cgccggctga tcgaccgctt cggcgatctc aagctggcgc
    48481 tggcggccta caacgcggga ccgggcacgg tcgagcggca cggcggcgtg ccgccctacg
    48541 ccgagacccg ggcctacgtg gagaaagtgc tggattcggc ccgggcgctc gcggccgacg
    48601 ggggggctcc ggacggggag tctaaagccc ccggcggcgc gcgccgataa caccacaggg
    48661 gtcttgcagg atgcagaaac atggtggata ggctcatcga catcatcggc agggaagcgg
    48721 ccctcttcga acgctttctc cttttgctcg agcggcaaca gcgcgccctg gtcgccaacg
    48781 accaggaaga actcacgcgg gtcaccgatc tccagcggga gacgctcgcg gaaagccggc
    48841 ggctgcacga ggagcgggaa ggcgtgctcg ccgacatccg gcggacgaac gcgatcgagg
    48901 gcgacctgac ggtgacgcgg ctggtggcga tggtcgatgc cgaccgcgcc gaccgcctcg
    48961 ccgcgctccg gggcacgatc ctcgacctca acgagcgaat actccggctc cggaaccaga
    49021 acgtcctgct gctcaaccgg tcgcgcgagt atatccggcg gaccatggaa ctgctttcgc
    49081 ggattgcgag cccccgggcg gaaacgtacg cgccggcggg cgccgcgggg gccgaggccc
    49141 gcacggtcgc cctggatcgg agggtgtgat gtccggacta atggcgggac tggaaatcgg
    49201 aaaacgggcg ctgctgtcgc accaggtcaa cctgcagaca atcggccaca acatcgccaa
    49261 cgtcaataca cccggctact cgcggcagcg ggtcacgatc agctcgacct acccgtcgga
    49321 aacggccgcc ggactggtcg ggaccggcgt cacggtcgag gatatccgcc acgtgcgcga
    49381 cctgttcctc ggcgaccagt accgccagga aaacaagacc ctcggccagt ggacctacaa
    49441 gcagaaaatt ctgagccagg tggagtcgct gttcgccgaa cccaacgaca acaccctggc
    49501 cgaccgtctc aacgagttct gggattcctg ggacgacctg tcaaaaaacc ccaacgcgac
    49561 caaccgggaa ggggtgatcg gagcggcccg cctgctgacc gagagtttcc acaccctcgc
    49621 ccgcgacatc aacaagctgc gcgattcggt cgacgccgac ctggccacct acaccagcca
    49681 gatcaaccag acgaccggcg aaatcgccgc cctcaaccgc cagattgtca cgcaggaagt
    49741 ggacgggtcg cgggccagcg atctccggga ccgccgcgac cagttgatcg acgagctctc
    49801 ttcgctgctc gacgtgaaca cgatcgagga tgcgaacgga gcggtgcggg tgatggtcgg
    49861 ggcgatggag atcgtgaccg gtctggacac gatcccgatc gcgacgcgga ttctcaacga
    49921 caacggccgc atgacccacg aactggtgtg gcggaatacg gcggtcgaat tgaaaaatgt
    49981 gaacggcgag ctgaaggggc tgttggacac ccgcgacaag atcatccccg gttacctcca
    50041 ggagctgggc gcgctcgcgc gcaccctggt cgagcaggtc aacgcggtgc accggacggg
    50101 atacgggctg gacgggtcga ccgggctgaa cttcttcgat agccgctaca ccgacgcaga
    50161 gtcgatccgg atcaacgagg atctggtcgc cgcgccgcac cggatcgccg cctcggcgag
    50221 cggcgaggtg ggcgacgggt caatcacgct ggcggttcaa aacctccgca acgagcgggt
    50281 gatggcctcg gggaccacca ccatcaacga ctactacagc agtctcgtgg ggcaactcgg
    50341 cgtggaggcg caccaggcga cctcgttcgc cgagaacagc gccctgctcg tgcaccaaat
    50401 cgagagtgcg cgccaggctg tccagggggt gtcgctcgat gaagaaatgg ccaatatggt
    50461 gaaattccag cacgcctacg acgcggcggc ccgcgtcatc acgaccatgg acgaggcgct
    50521 ggacacggtt atctccagga tgggggtagt cggacgataa caaggacgag tcctcaacgg
    50581 taaccaccac ggagggtact gtgctgatac tcacaaggag gttgggcgaa tcgatcacca
    50641 tcggagactc catccgcgtc tccgttctgg ggatccacgg ccgccaggtc cgcatcggga
    50701 tcgatgcgcc gaacgacgtc gtcgtccacc gcgaggaaat ctacgtgaag atccagcagg
    50761 aaaaccgcaa agcgtcgaag tcgatccgcg gggatctctt cggcatggtc aacctgctga
    50821 aagacaagat cacgggcaag gaacaggaga aggacaagcc ggcgccgatc gattaccgga
    50881 aaccctcgcc caaacggaaa ccgggcgggc agacggaccg gagtaaggac gagagctgac
    50941 ggcgggtcgg ccgagagggt gacgatgcga atctccacca acatgctcaa cgaccgcgtg
    51001 gtcttcaaca tgcagcgctc gctcgcgcgg tttctgcggc tcgaaaccca gatgtcgtcg
    51061 ggacggcgca tcaaccagcc ctcggacgac cccgtcggaa cgcagcgcga cctgggctac
    51121 cggacggaac tcgccaaagg ggagcagtac cggagcaaca tcgagcaggc gcagacgtgg
    51181 atgcagtcct acgacacgat cctcggcgac ctcaaggaca tcgtctcgag cgcgaaagag
    51241 atcgcggtcg cgatggccaa cggcgtctac gatgacgtga cgcggaacgg cgcggcggcc
    51301 gaggtcgagt ccctctacga gcggattctc cagctggcca cgagcgaact ggaggggcgg
    51361 caggtgtttt caggccaccg cacgaccacc aaggcactca cctcgaccgc aaccggcgtc
    51421 gtctaccgcg gcgacaccgg ccaaatcgac taccagatcg aggcctcggc ccgcctcacc
    51481 gtcaacctca tcgggtcgga agtgtttctc cggcagttgc ggccggtcgg cgccgaggcg
    51541 gacctcgacg cgggcgtctc cggcggcacg ctcgtggccg atctgcataa cggtgcgggc
    51601 atcgacacgg cgcccgggac attcacgatt acggacctca acctcggcct cacgtcaacc
    51661 atcgacgtgt cgggggcagc gaccctcaat gacgtcatcg cggccatcaa cgcgcagctt
    51721 gcggcggacg ggatcaccag cctgaccgcc cggcccggag ccgaggggaa caacctgatg
    51781 ctcgacacga gcgagaccgg gttgatttcg gcggccacgg cgctcgcgcg cctgaacagc
    51841 ggcaacgggg tcgacctcca gccgggccag atccgggtga ccgacggggc ggggatcaac
    51901 gaggtgatcg acttctccgg ggcggccacg gtcggcgaca tcatcgccgc cttcaacgcc
    51961 cacatgagcg cggccggtta cggcggcgtg accatgggcc tcaacgcggc cgggacaggg
    52021 cttgcgatca ccgatgccgg ggcgcccccg ctgggcctgc ggatcgagga agtcgcgccc
    52081 gaccagacga cggcagccaa tctgggcctc gcgggaaccg tggggtcgct cctggtggga
    52141 gcggacctgg cgccgacggc cgcgttccgg gtggccgaga cgaccgggac gacggcgacc
    52201 gacctcggcc tggcgggcga cattacgggc gacttcgcgg ggaacgacct cgacccgcgc
    52261 ctgcagccga cgacgccgct cgcgtcgctc cagcacggac tggggctggg cgagggggag
    52321 atcgtcctcc gccagggaga gcgcacgcgg acgatcgcgc tcgatgcgcc ggggatggtg
    52381 accgtgcagg atctgatcga cgccctcaac ggctgcggcc tggatattac ggctgcgatc
    52441 aacgccggcg ggcggggaat tcaaatcgac aacaacgatt cgacgcgctc gctggtggtt
    52501 gcggacgtcg gaaacgggcg ggcggccaag gcgctgggcg tctggggcgg ggccgacctc
    52561 atgggctcga tgctccttct gatcgagtcg ctgcgggcgg acgaccagga ggggacaggg
    52621 aggttgctgc aaaatctgga tgacgggatc gagcacctgc tcaaccagcg ggcctcggtg
    52681 ggggcgcgcg atatccgcct ggaacgcacc cacagccgcc tgaccgacct ggagctcggg
    52741 ttcacgaagc tcctgtcgga ggtggaagac gcggacatga cgaagctcgt cacggacctg
    52801 gccacgtacg agaacaatta ccaagccgca ctcatggcga gtgcccggat tatccagccg
    52861 agcctgttgg attttctgca ataaggcaag cggagaggaa atgggactat ggtagtgcag
    52921 acagaacgct ttggtccgat cgaggcgccc gaggaaaagc tcatacgtct gcagaaaccg
    52981 attctcggtt tcgaagggtg tgcgacgttt ttcctggtcg agcaggatga gttccggccg
    53041 tttctctggc tgcagtcggc cgaccgtccg gacctcgctt tcatcgtgat caaccccctc
    53101 ctggtgcgcc cggactatcg gatcgaagtg cacgccgaag aggtgaggga cctggagatt
    53161 acctcgctgc ggcaggtgga gacgtacgtg atcgtgacgg ttccggaaaa cccgcgggac
    53221 atttcggtga acctgcaggg tccgatcgtg atcaaccggg gcaacaattt cgccaagcaa
    53281 ctggtgctcg tgaactcgag ctacagcgtg cggcaccggc tgctgccggc ggccgacgaa
    53341 gaaccggtgc gcgaacgcga gaccgtcgaa gtctagggcg gcgcgggccc cggcgcactc
    53401 gggttggccg gcggcggcgc tcgcacgcgg agagaggcaa acatggcaaa agcgacagga
    53461 cgcatacggt ccggccgtcc gcggcgcgcg accgcggggg caggtgtgcg gcgaaccggg
    53521 gcggcgcggt cgcgtcggaa ggccgcctcg atgcggccga ta
//
LOCUS       DAOPOE010000007        53371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_796_length_53371_cov_5.722354, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000007 DAOPOE010000000
VERSION     DAOPOE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 53371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53371
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_53371_cov_5.722354"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKM94_01965"
     CDS             <1..575
                     /locus_tag="PKM94_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD65430.1"
                     /translation="LPDVYVPCEVCKGKRYNRETLEIAWKGRNIAEILDMTVDEALAF
                     FENIPRIKNKLLTLREVGLGYIHLGQQATTLSGGEAQRVKLAAELSKVATGRTLYILD
                     EPTTGLHFEDIRMLLGVLNELVDRGNTVIVIEHNLDVIKTADWIIDIGPEGGDEGGRI
                     ISVGPPEVVARDKHSYTGVFLNAVLNGKAL"
     gene            611..1504
                     /gene="mutM"
                     /locus_tag="PKM94_01970"
     CDS             611..1504
                     /gene="mutM"
                     /locus_tag="PKM94_01970"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOD65431.1"
                     /translation="MPELPEVEVVCRGLRRVVVGRTIGRVTVRAPKSSVTVSPSLGAR
                     FLDEALRGRRIESVDRRGKNILIRLAGRRTLWVHLKMTGRFLWLPRTEPIAAHDLVIV
                     DFLPEAGADDGHHLRFNDYRRFGRWRLYPDDELDLQEGLRALGPEPSALSADDFAALC
                     RRRPRRIKAALLDQSFLAGIGNIYADEALYAARLHPLRLTTAVSRRKLRELHTHIVRL
                     LRRAIHLMGTSVDSYSGVNGRPGRFQSRLQVYGREGKPCGRCGAAIVRRKIGARSAHF
                     CPRCQRAGEAAPGLPRKNPPA"
     gene            complement(1543..5592)
                     /locus_tag="PKM94_01975"
     CDS             complement(1543..5592)
                     /locus_tag="PKM94_01975"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOD65432.1"
                     /translation="MIKWGKYSPVFVALVAVLVLGAVALSGIGNPRDLDKERRAADPV
                     NLKAAINAARPAALDPFEVEKAQMEADLTAREAAESAAMDQAKGEEPVRPTRPSRESD
                     RNRMAGPNTILRPAVNYEPRTIFLQEDFETEIFPPTGWDSINTDPGYGWFLGTYSGGG
                     TQCALVTWHAAGYQQDEWLITPMMDVSGAGSLLRLEFDMLKGYDYPHDFKVYVSFDGT
                     AFTEIWDSYDVAYPTFTWYGVTVDLSAYAGGAPIWLGFQYYGMDADLFGLDNVVVTDD
                     APPAGRCCYGEPTAPNCQDVTEVECTALGGNWSAGLNCTDNPCPVAGPNDNCAEVTPM
                     TLPYTFMGNNEAATFDDYCQYFGDYPNVWIAFTITECSDITISYCNTASGWLNGWLNL
                     VTDCACPEGSLISGATYDFGCPNGNANIYFSHLAAGTYYYPIMLDPTNGAIGDYVVDV
                     TAEACPPVTPGDNCEDPFVVALSSADLPYTVAGQYTCGRINDYDATVCMGYYDGGEDL
                     IVRVDVADAMFVNITLDPKGTTWSGMGISQDCTFATCLGFVGTGGGTAKTIYNVQLDP
                     GSYYIMVDTWPTPDCIPELDIIFEEAPPPNPGDNCANPVKVDIPVLPFVDTNTTCGRI
                     NDYANTCLGSYDGGEDIIYEITALADVTVNIAMDPQGTTYTGFALAGDCAFTTCIGFV
                     TGSATTVKRLTCVNLTAGSTYYLIVDTWPSPNCIPSFILTITDTTCAAIENDLCEDAT
                     EVGEVTDLPFSTVGTSHDGPTGCMTSPNIWFAYKPSAAGLATISLCGSEYDTRLGVYT
                     GTCGALTQIGCDDDACDPGLQSVLYDIPVDPAMTYYVEVGGYSTNVGNGILNITLAPP
                     CEFTCTGGTPEGEDCIEDYADDVTNGGCNMDVPVFGSIGPNEKICGTFSTYTPDGSAQ
                     YRDTDWYILSLDDWYSVTLSIETNFDAVFGWIEQITPGIASCDNITGYIAPYLAVGDC
                     EPSALEPLTLAPGDYLIFVSGQTYTGFPCSMGPWEYALTATSVPAEPTPCAASGGCDE
                     YISQVQIGTIDNSSDCTEYGDYTAISTDVEVGGSYAITVTNGSGYSSDQCGVWIDWND
                     DVVFDASEMVTLDVSTGVGPYTGTVVVPEGAVEGGVTMRVRIMYTGTLAPCGTTSYGE
                     VEDYTLNVGGAAPTFMVAPDPILVLEKFALNPTWGDVYIGDGALPDGAAAAMENITVA
                     LGGCPVTVGVTQVIPGGVGEIPGDVMDVKFSLKEYVLCEEARQGGLIWDDIDSFFDVF
                     YEIDGVPGTLNLQVVVRGHRSGDLNLDGNVNVTDLTMFVSYLFRGGDAPLEPAVANVD
                     ASPNQTPNVADLTYMIGYMFRGGPMLKHQ"
     gene            complement(6195..6983)
                     /locus_tag="PKM94_01980"
     CDS             complement(6195..6983)
                     /locus_tag="PKM94_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD65433.1"
                     /translation="MLLFTHDKLRLAEHFRRDPVRFAYHLGDLDDALFSRCQWAVSHH
                     KSTRIDECILIYRAPSGPTVLAFGLSERLEQLLAEALDLLPPRFTAHFASSCRPVFAR
                     RYDEEPVGRGIRMRLTALRSGPSGDEHSIRRLGAADAADLQRFYREAYPDCRFDETMV
                     ATGKYFGWSVDGRLVGAAGVHVYCPDHGVAVIGNVATDPSERNQGIASRLTARLAQEL
                     ADEQLLVCLNVMVDNLPARRCYEKLGFETAFEYEEARFTFARTA"
     gene            complement(7011..7214)
                     /locus_tag="PKM94_01985"
     CDS             complement(7011..7214)
                     /locus_tag="PKM94_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOD65434.1"
                     /translation="MRFELDKNATERVVIEDSEYKGHHLVQLRIYFLADDSQWLPTKK
                     GVSFRRDQLDEVIDALQKIKAGG"
     gene            complement(7264..7761)
                     /locus_tag="PKM94_01990"
     CDS             complement(7264..7761)
                     /locus_tag="PKM94_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011822382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOD65435.1"
                     /translation="MASEKFKTHVVKVEIPEGLNVIFGMAHFIKTAEDLYEALIEASP
                     TIKFGVAFCEASQKRAIRTEGNAGDLIERAAKAAFDVGCGHTFFIYLKDGFPVNVLNR
                     VKSTSEVCRVFCATANPLEVLVAETDLGRGVLGVIDGQPPLGIETEADRAERREFLRR
                     IGYKR"
     gene            complement(7751..8563)
                     /locus_tag="PKM94_01995"
     CDS             complement(7751..8563)
                     /locus_tag="PKM94_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65436.1"
                     /translation="MNATRGALLALLVTAAGLSAAPPESPLIDKEALIEQFLAVDGRQ
                     RAQLHDLVLDAEYYEGRISEGEFQAEARFLKRVFIKFAKDTALYREEYREYYRDGVQQ
                     SPEETAKQARDRLEKNRKRNARNIAYPMLRPFRPENRKDYEIAYVGLDEVEDTTCYHF
                     RVTPKVKTDEHIAGDYYFETDGFHLVRVDFSPARLVKKAMFKLDRLDMSIVYGPAPEG
                     FWLPKQFDVAGRGKAALFFGVNFAGTEYYRNPQINVGQSDTLFKSEDADNGE"
     gene            complement(8560..10542)
                     /locus_tag="PKM94_02000"
     CDS             complement(8560..10542)
                     /locus_tag="PKM94_02000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD65437.1"
                     /translation="MKRLRLGAIAVLLAVAGYYAYDRLTPPTRIDKLAQIIHLEDRRE
                     LSGRLKSYLGDSDPAVRARAALAVGRIGGPGSAEPLLDLLADSSWEVAGAAATAIGFC
                     GQPEFALRLLETADNLPPRITARAVESAGRLADTGKAEVIDALLAYLSHPAPEVRERA
                     VRGLFRAGARSAAAEVIGLYGEDFDEPVRQACLYFLARFDIAAGKEIFASYLADPDPN
                     LRGLAVRGMGAVAGEEAERYVSIALNDNTDRVVAQAIAELAGRDPDRVSRLLQKKLAV
                     EEDPKLIVELIEALRRLGSSEGIATAEEALAAYATPSIAAATVKYAAAARGGRAMDLI
                     DSLLRGDSPFERAAAAEALGLIRERTVLPRLTTLLADRSPQVRAAAFGMLMETDSANG
                     DFYIDQALHDSDFVLQALGVEAAGKRGDPQYLPLFRTLMEPAAGTAADVRRSVVGALP
                     PFLSSRADSAARELLRLALRDAEAAVRRDASQVWDSLVTAPKPAVSLLADTRLSERAI
                     ARALAGYAANPRALISTSRGNLAIELRFDVAPLTVMNFIELAEAGFYDGLIFHRVVPN
                     FVVQGGDPRGDGWGGPGYYIRDEYSAETFERGAVGIATSGKDTGGSQFFITLSPQPHL
                     EGRYTLFGRVREGMETADRITIGDTLRTVRIVKDAP"
     gene            10706..11071
                     /locus_tag="PKM94_02005"
     CDS             10706..11071
                     /locus_tag="PKM94_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD65438.1"
                     /translation="MYNPTVMDHFQNPRNTGDLDRPDGIGQVGNPACGDVMRIFIRVE
                     AGRIADIRSQTFGCAAAIACSSMASEMVLGKTLDEAFALTRDDVAQALGGLPEKKMAC
                     SNLAPDAIRAAIEDYRARR"
     gene            complement(11092..12033)
                     /gene="ilvC"
                     /locus_tag="PKM94_02010"
     CDS             complement(11092..12033)
                     /gene="ilvC"
                     /locus_tag="PKM94_02010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOD65439.1"
                     /translation="MKDLSTGGPVAVIGYGSQGHAVAQNLRDSGFDVIVGLRPDSPRR
                     ADARRDGMPRIDGIGAAVGQAGGVVFAFPDHGVVYRREIRDRLRPGAALVFLHGLSVH
                     FGLVEPPEDADVILIAPHGPGVAVRANYLAGDRSMSAFVSVHRDSSGQAGPTMFGLAA
                     GMGFDLTRLVPTTFEAEAVGDMFGEQAVLCGGLAALITGGFEVLVKNGIPPDAAYLEV
                     AYQLDLIVALIKKHGIRGMFERISLTARYGSLETGPFLIDEVVKARMQQVYDRIASGE
                     FVSRLTRLSEAQIDELGADLARLTDEKLEQAARKFKG"
     gene            12097..12999
                     /gene="nadC"
                     /locus_tag="PKM94_02015"
     CDS             12097..12999
                     /gene="nadC"
                     /locus_tag="PKM94_02015"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOD65440.1"
                     /translation="MRDQSLWLVELVKAALGEDVGPGDLTSMGCLEPNPAKARIVAKQ
                     DGVLSGLRPMLLVFGIVDSANTVRVFKNDGDRFAKGETLVEIEGFNQSMLAGERVALN
                     FLSHLSGIATLTAKFVAAVAGTGARILDTRKTTPGMRVLEKQAVAHGGGCNHRMGLHD
                     MVLIKDNHIASAGSITAAVNRMRDFLLSPDFRAQFRRPAREIELEVEVVNERQVAEAI
                     GLGVKRLLLDNQSVDSLRALVAKARAMDPEVKLEASGNVALDTVRAIAETGVDYISIG
                     QLTHSAPVADLSMQIVEQPLDEPA"
     gene            13064..13978
                     /locus_tag="PKM94_02020"
     CDS             13064..13978
                     /locus_tag="PKM94_02020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD65441.1"
                     /translation="MAQLTEELGAPRTELEAAIAQLETWGYTFKTARNRLHLTATPDS
                     LTATELKWGLNTGFIGQRLLAYNKVKSTNDIVSREGAAGGVEGTVAVAEQQTGGRGRL
                     GRGWHSPKRKGIYLSILLRPEFLPEHAPGLSLMTALALAKTIEATEKIRIKIKWPNDL
                     ILNDRKVAGILTELAAERRRINYVVIGVGINANQDEQDFPEELRQSATSIRMAVGHEI
                     SRVRLVQNFFREFEREYQLYCREFLAPSLPRLRRYSTLLGEKVRLASGLQILEGVAVD
                     IEPGGGLVIERGGKRHVVTAGDVTVVKE"
     gene            14199..14789
                     /locus_tag="PKM94_02025"
     CDS             14199..14789
                     /locus_tag="PKM94_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HOD65442.1"
                     /translation="MNQRAILVGRGRLVAAGVVMVLAMPLAWLWAQGAPTYTAEQVKR
                     GEYLVVIGGCHDCHTPKKMTERGPEPDFARSLSGHPAQEKLPAVPAGAITPTGWMALT
                     NGNLTAWAGPWGVSFALNLTPDVKTGIGGWTEEMFIQALRTGKHLGMGRPILPPMPWQ
                     NLSHATTEDLRAIFAYLKSLPVIENRVPDPIPPAVH"
     gene            complement(14928..15182)
                     /locus_tag="PKM94_02030"
     CDS             complement(14928..15182)
                     /locus_tag="PKM94_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65443.1"
                     /translation="MTHGSRNLLSSLGRRRLLGLLLVAGGAVLSGCGLRSAGEKARAR
                     GRILARGGGASIPAGLEVTLQFPLLEAIYGRRSRRFFRGA"
     gene            15487..16308
                     /locus_tag="PKM94_02035"
     CDS             15487..16308
                     /locus_tag="PKM94_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65444.1"
                     /translation="MTGLSAVALAVAGMAAIAGTEAAPPGAALLPPSLNKLYPPEAPA
                     PVYLIEMHALAAPLSGMMSDLMEGDHANAMANFEAFRAQYAKVAELVPEWRHEFPTGP
                     VETLATALRSGDQGAVMRAGDAVGRACHGCHLQTMVPAQHRFHWGDFGAISVTDPVIG
                     QDMPFAQFMHMLDGDLAGIGNDLKQGQPENARRHAEGLAARYATLEEVCGVCHDTDRK
                     YYVDEKVMQMIADLPAALENGKPENLGALLQGIGQESCQKCHLVHIPAAYARAAH"
     gene            complement(16395..17525)
                     /locus_tag="PKM94_02040"
     CDS             complement(16395..17525)
                     /locus_tag="PKM94_02040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD65445.1"
                     /translation="MKRWRILVVDDEPGVLRAVQRVLGSAHDLTLCDSSPAARDLARR
                     SRPHLALIDIRMPHLDGFDLMTQLKEDDPDLEVILMTGSMDDTDEKLVRAVRNRAFYY
                     ITKPFDRDALLALVDRCLELKRLELSNRAYADHLRRQLLAAGAFQRTMLPPASAHLNG
                     VELRAGYYPCDQVAGDYYDYAVTGGDLVTLLVADVVGHGASAAMFTALVKSAFHRAAD
                     RGYAPLRVARILLDDLSAFESDQYVTLLCARLSRSGRTIEYVNAGHSGGLIVGGDETL
                     LPLEVTSPLISPVVPPSALRTATIPLPADSLLLLFTDGAAEAWSDDEMFGLERLYALA
                     RTGWACRDALPQHLQDGVRRFLGDRSQTDDITFLAAWPASAR"
     gene            complement(17522..19213)
                     /locus_tag="PKM94_02045"
     CDS             complement(17522..19213)
                     /locus_tag="PKM94_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65446.1"
                     /translation="MFDGTIDKTHADLYKVLFDLRGTLRDYGEPRKMLIYGMRRAVEF
                     LAADAGAVATLSASAHTADLLFTTRTSTEWNVGLLNDFIKRREPRIPDDVVLARVERR
                     AATWGAIAVRLPHPKEAFHLRALGLIAEAVREGIAQYDDRQIRRLRHRLEQKVVNRRE
                     PKDIIYLILHGIRSLTRYDHSATFLATNGPDGPLQIIAEQIAWTKAKSRRIGLTVPLA
                     AELRDELSRGGIHLHRQGGHGWQPVFGAVRTPGLIPALCCTCPRGASAPEENAVLCAV
                     IPAPDGSLGILKIAFQRADIPGAYEIELVENFTPLLSLALQFHHRTEVLTEQVVQAER
                     KSALADLSRGIAHDVNNAIGSVLPLAQQIRADIARGELDQRELLADLDVIESGMLACR
                     RIFAGLLSVARSGDRTIGEANLRRAVDGALNVVRSRLDRHSIAVELNIPPELSAIGAN
                     QGHITQVFLNLFANSIEAMPHGGRLAVSAVEKGPEIEARVEDSGGGIPPDLLDRVADA
                     FITTKESGHGLGLAVCRGILWNIGGELRIDSTVGQGTCVTLRLPTQSSRRQERLP"
     gene            complement(19489..19914)
                     /locus_tag="PKM94_02050"
     CDS             complement(19489..19914)
                     /locus_tag="PKM94_02050"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HOD65447.1"
                     /translation="MEAQVLVPQALNYWAILVAAAAYFVLGALWYAPPVFGKVWRTGI
                     GKTDEQVKADFSPWNLVWTFIFSFVAAYGVARLVQWSGGTSAGDGLLVGFLAGVCFAA
                     ATVAMHHVMEKRPCSLTVVNALYSIVGFLLMGLIVGAWR"
     gene            complement(20013..21674)
                     /locus_tag="PKM94_02055"
     CDS             complement(20013..21674)
                     /locus_tag="PKM94_02055"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HOD65448.1"
                     /translation="MASILNLLAQNDLLLLFVIIGLGYLIGNIRILGFKLGVAAVLFV
                     GIAFSALDKRLILPEQIYIVGLVLFVYAIGLQAGPGFFTSFHRRGLRISAIAIVILTA
                     GAVVAGVIGHFLNLPATSIAGLYCGALTNTPALAAAVEATANMAKALPPDKVQFYLNS
                     PVVTYGLAYPIGVAGMILWMFAFAKIYRVDFSRETEEVKREADAEAIASRTFRVTNPA
                     VQGQTVEQFFAAFEQPQFVFSRIKKGDLVQVVSPETVLEANDLIVAVGPSKALRTAHL
                     LLGSESNETLAERRDETAYKWFFVSNPLAVGRSISELRRQRAYVGTVTRLRRGDVEFV
                     PSPTTTLEMGDRVLVVSPRLEVDRIAGYFGDSVKALSETDYLSLSAGIVLGVFLGMIP
                     IPLPNGTAFKLGFAGGPLVAGLVLGRLGRTGPIHWSLPFNANLALRQIGLVFFLAAIG
                     TKAGVGLTATVETGALGMIVSAALIVSFVAAATIIAFYRYLKLPMSATFGLVAGIQTQ
                     PAVLAYANQQAQNELVNLWYATVQPASMIAKILLAQIIVTTLLFR"
     gene            21908..22060
                     /gene="rpmH"
                     /locus_tag="PKM94_02060"
     CDS             21908..22060
                     /gene="rpmH"
                     /locus_tag="PKM94_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD65449.1"
                     /translation="MKRTFQPSNRKKLNDHGFRKRMASKGGRLVLSRRRAKGRKRLTV
                     KVARKK"
     gene            22044..22409
                     /locus_tag="PKM94_02065"
     CDS             22044..22409
                     /locus_tag="PKM94_02065"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD65450.1"
                     /translation="MPARSNQSSRCLPRCLSLKQRRAIEDVVQRGRTLRGAAATFRWQ
                     PADRFGYVVVVGRRHGHAARRNRIKRLLREALRLNRHRLTREVRIAVLPRLYGDALRF
                     DILHAEFGRIFEAINRHAD"
     gene            22360..22656
                     /gene="yidD"
                     /locus_tag="PKM94_02070"
     CDS             22360..22656
                     /gene="yidD"
                     /locus_tag="PKM94_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOD65451.1"
                     /translation="MRNSVVYSRRSIATPTNPRPSVAARLLILLVRIYQATLSGLFAG
                     SCRFYPSCSHYSVEALRSHGALSGLYLSVRRLLRCHPWHPGGYDPVPSAKETRE"
     gene            22698..24410
                     /gene="yidC"
                     /locus_tag="PKM94_02075"
     CDS             22698..24410
                     /gene="yidC"
                     /locus_tag="PKM94_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOD65452.1"
                     /translation="MDKKTIIIVVILGAIIIFYYPILQFLGLYEPPRPSSPGTAPDST
                     AAVDSSAPVPVIKPEAVQEVTVKPQPVAEIPADTVGADTIAVETEFYRILLSTRGGGP
                     VSIQLKEYTYIGGANVEMLPECEAATPNAVFAGGTFSTAALPFQASLAPGRYSVARET
                     LPISFTYTNPGGGIIRKTYFFYPDQYHYDLTLTVEDTRALGFERSYTLWWNTPLGITE
                     SQARADYSAMQAVTMQGGSRATLDEFHDNQLAQSLDGYTEWVGVRNKFFSAILIPRSR
                     EAQAAVAEGSKQMLATPEGRIEQRRITAGAVMTFAGVETISDSFTVYAGPLDYLRMDD
                     YGVDLEDMLDIGTMPFFGAILKPFAIAVMWLLPKLHDIVPNYGLVIILFAVLVKIITL
                     PLSLKQFKSMQAMRDLAPKLEDLKKRYKDDNQGLQRETMKLYKEHGVNPMSGCLVMLP
                     QMPLMIAMFRVFQATILLRHEPLFWFVTDLSRGASGLTDPYIILVVLMVVTQMLSSLL
                     TMSGGGQQQKMLTYIFPIMLGFFLYSLPSGLIIYWTAFSVLAVVDWLVFRRNRAKNPQ
                     IKTA"
     gene            24471..25817
                     /gene="mnmE"
                     /locus_tag="PKM94_02080"
     CDS             24471..25817
                     /gene="mnmE"
                     /locus_tag="PKM94_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOD65453.1"
                     /translation="MITPPGEGGIAALRLAGPASLAILSRHFRPTQVKSPASPWLLRH
                     GLFTDREGASIDEVTAVYMPEGQSYTGLEQVEIFCHGGHLVVRQILHELLTAGARAAE
                     PGEFTRLAFLSGRIDLARAEAVAEVIAANTEVSLATARNHLLGAYTERLEQMRQEIVA
                     VMAEVEASIDFPEEEIEPAMAAGQRESIDQVRGKILQLAATYRGGRIIREGYRVTIGG
                     RPNAGKSSLFNRLLRQERAIVAATPGTTRDYLSEWIDLHGYAVNLVDTAGIRRGGGAV
                     EQAGQTSARKIMEEADLVVWLVDLSAKDWQKQLGEDLASIGGLPVVIGANKIDLVGAA
                     PAISETPAEIETIYLSCKTGEGIDNLVGHLVAHINEGMPDFTSGMIVTSARHRQKLET
                     AETALRSASEKISLKESPELVAFDLRQAAGALEEITGRIYNEDILDQIFSKFCIGK"
     gene            25911..27785
                     /gene="mnmG"
                     /locus_tag="PKM94_02085"
     CDS             25911..27785
                     /gene="mnmG"
                     /locus_tag="PKM94_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOD65454.1"
                     /translation="MMVNRDFDIVVVGGGHAGVEAALAAARMGKSAAFVTMDPHRLAL
                     MSCNPAIGGIGKSHIVKEVDALGGVMGIAIDATGIQFRRLNLSRGPAVWSTRAQADRI
                     GYNRFIVELVRNCPKVSVIPGLAGEILTERETVVGIRLESGDEISCRAAVIATGTFLG
                     GLIHIGRKQIPSGRIGEPAAYRLSESFRSLGFEIGRMKTGTPPRLDGDTIDWGACQVQ
                     PGDEPVPFFSPRSPRRVFQQVCCHLTYTTSATKAIIGENFHLSPMFSGQIKSRGPRYC
                     PSIEDKFFRFADKERHQLFLEPEGNGTNEVYPNGFSTALPEEVQVKAIRTVAGLENVR
                     VTRPGYAVEYDYCPAHQIKPSLETRRINGLFLAGQINGTSGYEEAAGQGIMAGINAVL
                     RIEQEAPFVLDRSEAYIGVMIDDLTTRSTTEPYRMFTSRAEYRLTLREDNARDRLQSY
                     ARRFGLIPETVLAGFDDLQRRTDAVVAMIPKIRVRVDELGEAGARFTRVETISLDNLL
                     RQPTLSINEAVAWLRKFPGRFPDDQEALERAAIVVRYSGYVDKQQREIDKFRRMERET
                     IPDDFDFAGVSGLKKEAGEKLARFRPTSLGQAGRIEGITPGDVAVLSVYLQRHKALSA
                     "
     gene            27782..28432
                     /locus_tag="PKM94_02090"
     CDS             27782..28432
                     /locus_tag="PKM94_02090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD65455.1"
                     /translation="MNGTSEGIRLDVAAFLRERDPNNRLTPYFELLARENSRVNIVSR
                     ETLPPTAPSGRDRFEGLRRLTAESIYPITELHRAQTRSYLDIGSGGGFPAIPIVLTAT
                     ISDTLLVERTRKKAAALRRILMELQLRAEICTESFESLVLKEGSCDLITLRLVRLTDR
                     LAARILPLLAPGGDFVYYALADISADSSRFLTRQLRFTAGGGRDFHTATIVTRKPE"
     gene            28520..29278
                     /locus_tag="PKM94_02095"
     CDS             28520..29278
                     /locus_tag="PKM94_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65456.1"
                     /translation="MARIIAIANQKGGVGKTTTAVNLSSCLAAAELKTCLIDMDPQAN
                     STSGFGFDKRTIETSIYDVLLGSATLREVLRETELSYLHLAPSSIALVGAEVELVSVM
                     SRETRLRAALAPVLDDYAYILVDCPPSLGLLTVNALTAAHSVIIPVQCEYYALEGLGQ
                     LMNTIRLVQEHLNPTLQIEGALMTMYDGRLNLSRQVLDEVKKHFGGKVYDTVITRNVR
                     LSEAPSFGKPIILYDILSTGAENYMALTREVLAR"
     gene            29275..30126
                     /locus_tag="PKM94_02100"
     CDS             29275..30126
                     /locus_tag="PKM94_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD65457.1"
                     /translation="MKKIVLGKGLDALIPVESGEAGGERRMRQVAVDLIAPNPLQPRR
                     EFDEAGLLELAESLERNGMMQPLVVRQDGSTYTIIAGERRYRAARLAGLSQVPVIVRD
                     ESDDARMLELALAENLQREDLNPMEAAEAYRTLMERGGYTQQALADAIGKSRAAVANA
                     LRLLTLPEPVRQMIRAGRITEGHARAILQIEDEQGQIEMAERIAEQSLTVRAVEEETR
                     RPARAKGRPAGRRLPPAMAEAETFLKQTLGTAVHIHHGPKRGRIEIEYYGVDDLTRIL
                     ELLAKLS"
     gene            30129..30914
                     /locus_tag="PKM94_02105"
     CDS             30129..30914
                     /locus_tag="PKM94_02105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD65458.1"
                     /translation="MAQSRYITVLFVPDGNRPQRGFRVRTWVVQAGGVALTMALLAII
                     IFFATYSKIMVRATMTDRLKEENEQLLKYQYKVRILEDNIAEMRQVVARLTSLAGIDY
                     QFPEIPSDSELFAAIDEAPAAAINRPATDFTMPAGLPIRGFITQNFEVKNDARYHSGV
                     DIACPVGTPVLATAAGLVIFADVDSTYGNMIVLRHNDTVVSVYGHLDRILARAGEEVP
                     AGSRIGLSGNTGISTAPHLHYEVRINDEPINPLENPYDQEKRQ"
     gene            30928..31335
                     /locus_tag="PKM94_02110"
     CDS             30928..31335
                     /locus_tag="PKM94_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD65459.1"
                     /translation="MNTLIGKDTVITGTVDVKGALRVDGTVKGKVICSDCVTVGTTGL
                     VEAEIESKEAIVAGHMHGNINATEKIELQANCEMDGDLRTKSLVIEQGAVFCGNCNMK
                     GTTASRKEFGFLPPEQNRETVANVIGDKKEVKK"
     gene            31723..32727
                     /locus_tag="PKM94_02115"
     CDS             31723..32727
                     /locus_tag="PKM94_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOD65460.1"
                     /translation="MAISGTQADLTAVENLNSAYRRIKAEIGKVIIGQERVIDQLLIS
                     LLASGHCLLVGVPGLAKTLLISTLARVLDLRFNRIQFTPDLMPSDITGTEIIEEDQAT
                     GRRRFRFVKGPVFAHIILADEINRTPPKTQAALLQAMQEHEVTAAGETYKLDEPFFVL
                     ATQNPIEQEGTYPLPEAQLDRFMFNVFVDYPSEREEREIVRSTTAVLDYQLNKILSPQ
                     EIIGFQQLVRRVPVSDHLVEYAVKLARKTRPHEPDAPDFVRNWVNWGAGPRASQYLIL
                     AAKTRAILDGRPTPGPEDVRFAAYPVLRHRIVTSFNAEADGVDTMEIIKRLLETVKEQ
                     "
     gene            33454..34224
                     /locus_tag="PKM94_02120"
     CDS             33454..34224
                     /locus_tag="PKM94_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65461.1"
                     /translation="MIEKIRISDLPPYGKTFVGIFAGLAVCMLLWVALIYTVDRGIVH
                     SDRLPAYLNADRAGQGAPTTEDSARNERLEQEEIAEEAEEVAVDSEAVLAPIWDSDLA
                     GREVHADSASAAARFGKVDSTIEAEIARHGVGPGTGYDYNPDREDFDVDEFEQPNLRR
                     NAGLAHTHINGQTLIYFTLGLVFLFTSAAPKIKTTVYWLLGSAVAAHAVGLSGAGFHW
                     FFDDLLAISGLVIVGTAGYMLFWIFADLGKKRATPAGE"
     gene            34240..35211
                     /locus_tag="PKM94_02125"
     CDS             34240..35211
                     /locus_tag="PKM94_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOD65462.1"
                     /translation="MPQAMNAHPPFAIAIHGGAGTILKEKLTPALEAEYLDGLRRSLA
                     AGYQVLDRGGRALDAVQAAVNVMEDSHLFNAGKGAVFTHEGFHEQDACIMDGAGRAVG
                     AAAMLRRVRNPINLARAILEYSPHVLLAGEGAEKFAQEHGVRLVEPEYFYTEQRWQQL
                     QQALAKERQEGGDQTRLDHSDDRDKTGTVGAVALDRAGNLAAATSTGGMTNKRFGRIG
                     DSPIIGAGIWADNATCAVSATGVGEYIMRAVAAYDIAALMAYRGLSLAEAADEVVTGK
                     LTALGGQGGVVAVDRAGTIAMPFNSPGMYRGYRYPGRDPVVKIFRDE"
     gene            complement(35282..36190)
                     /locus_tag="PKM94_02130"
     CDS             complement(35282..36190)
                     /locus_tag="PKM94_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD65463.1"
                     /translation="MLHSIWPRTGAAAVLLVVLAFACRGGGEGGPADASAALRVDSAL
                     STDSVPIYFEVHGSGDTALVFVHGWGIDGSFWEKQIPAFSDSYTVVTLDLAGHGRSGK
                     NRAAWTPHAYGADVVAVLDAAGIAHAFLVGHSMGGVIIAEAARLAPDRVIALIGADTY
                     QDFRERWTPDQAEQFLAPFRRNYDSTVAWFASVMLPENVDTAIAYRIIRSFQADDPAV
                     GLATLGNLLVYDALPVLREAGKPIRAVSSDSYPTNLAANREIEPTFDVRLMSGVGHFL
                     FLERPEEFNRLLGQTLAELRAGGAAP"
     gene            complement(36342..37307)
                     /locus_tag="PKM94_02135"
     CDS             complement(36342..37307)
                     /locus_tag="PKM94_02135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOD65464.1"
                     /translation="MIESEELKESVKTRLEESGIIGTMAERICHSLLSEKPPGLSPDE
                     YFHLVSISLLELLRARLLASCEPDRLVGPLDRGQLLDYDRILQGLLLTLERDLLRSHL
                     HMIRALGGAIAERDTGTNDHSYRVTIFAVRIAEAMGLPRQTIRSLIKGAFLHDIGKIG
                     IPDAVLIKPGELTPPEREIIKSHVSRGAGIIEVVTWLADAIDVIRYHHERWDGTGYLA
                     GLCGVEIPLTARIFAVADVFDALTSRRPYKLSYNFDQARDMVVAGAGSQFDPDVVRVF
                     AARAEDLYAEGHRAATRRLEEVIRRFIREYFAVEWDRAVQMEPSM"
     gene            complement(37455..39074)
                     /locus_tag="PKM94_02140"
     CDS             complement(37455..39074)
                     /locus_tag="PKM94_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65465.1"
                     /translation="MQKSPATAVTDSPPLAPAGPGRDRAWVRLPLALLLVIAAVHLAL
                     NLGINDGHLTYTLDDAYIHMALAEGIAHGHFGINPGEVCAPSSSILWPFLLAPFMLLS
                     FGAVVPFVVNLACAAATVLLMVRTMAHGFSAAGLANPVKYAAWVAAALVPVTNLIGLI
                     FSGMEHSLQLLLAAAVVHGLVCALSCQRVPRWLPAALIAAPLVRYEMFALTLPAAVCL
                     YLSGHRRVALVSALTALGLAAGYSGYLAALGLGALPTSVAMRSDVVCSGWSIAAITDH
                     FRGNLLSGEGTLLAVVFIPLAAVALDRSRRREHRLLAAAGAAGIVLHLSVGRLGGWFH
                     ARYDMYILLSTLLLTVFLASAPLGRFLQRAGGVRAAVLAVAAVALVAFPQLHRLSDLQ
                     IAASNIYQQQYQMHRFITEYYHGPVAANDIGWVAFQNDETVVDLVGLADSRNVGRRKE
                     ASSGQWMTEAAAAGGARLAIIYESWFAHIPDSWIPVADLFLGHRRTSAADRKVTFYAL
                     DRAAADSARILLTRFAPTLPKGVRLDLRPSE"
     gene            39494..39853
                     /locus_tag="PKM94_02145"
     CDS             39494..39853
                     /locus_tag="PKM94_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOD65466.1"
                     /translation="MNIYVGNLSFDTTEEQLRQAFSSHGEVTSVNIISDKYSGQSRGF
                     AFVEMPTKSEAIAAISALNGQEMNGRAISVNEARPREERGGGGGGRGGFGGGGRGGHG
                     GGRGGRSGGRGGDRGGW"
     gene            39953..40357
                     /locus_tag="PKM94_02150"
     CDS             39953..40357
                     /locus_tag="PKM94_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65467.1"
                     /translation="MTMAGQRKSFPCGHAGKGKYCHRCAAAEAERQKVAQIKGDWQSR
                     LRASPIPLDHLPKGVAENVLRVIEDLKSGKPYEDLKGKRLITMGQRDVISIPIQRRYR
                     LICRDREGKIEPIEAITHEVYNSRLSSGGWPK"
     gene            complement(40538..42457)
                     /locus_tag="PKM94_02155"
     CDS             complement(40538..42457)
                     /locus_tag="PKM94_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD65468.1"
                     /translation="MRTIAFVAILLLLVGGCGDETTNNIYNTDTAAGGVFGRVSPPEA
                     GVLVSAWQSEQIASTATDAEGYFTLENLPPGLYEVRFAASDDREKVLGGIAIEGAGFI
                     NLGSIILASSWPIVSFYPADGDTVVPAATSLELRSEVELDLSSLDDAVVITPPVSDSW
                     SRGYDYSGRSYIYALEPTSGLLPDENYHVHITEQLALADGGPWGDTLSFSFRTAPMAL
                     TEINSSYDLADLSPGHSGWLCDLRFNAYVDESTLLTAVRIEPDVPFTASLSQSNLYTV
                     RIEVEGDFACGADYSIIVDTALHCLAGAGRATPDTIGFHTEPLRVVYRSYNYSPNALP
                     PRPQTLFEFDYNSDLDPETAEDAVSVDPPLELQVEVSSDYYSDTRELRVSILSQPTPG
                     ETYTVTIDSTLASSHGGTTEADEMVVLKIQPLTVTQIAVSNGSGFYETQTTLSPFGAF
                     YARIRFNAEVDENALNDAVTIDPPLPGFWYFAESYYDVGYYDFFPTQPVQLFPEQSFT
                     ITIDGDAPLIGSAALGEDKVFAFGVAPVAVAGVYPAPGTRTFYWYDDPYFRFNAPMER
                     TSTESAIRFTTMTGTPVNGVFQWSDGDKRVSFNPTASPVVGQAYVFHVDTSAASVAGP
                     HLKYADSTFFTVGEY"
     gene            42701..45898
                     /gene="ileS"
                     /locus_tag="PKM94_02160"
     CDS             42701..45898
                     /gene="ileS"
                     /locus_tag="PKM94_02160"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOD65469.1"
                     /translation="MKFDSLKDNFTIPAAEEKILRFWDENDVFHKAQKAHHDRPHFVF
                     YEGPPTANGRPGIHHVIARTVKDLVCRYKHMQGFRVDRKAGWDTHGLPVEIEVEKQLR
                     LETKSQVLEYGIGNFNAKCRESVFTFLDQWNEITRRIGYWIDLDDAYVTLTNEYIETV
                     WWILKTFYDRDLLYKGYKTVPFCPRCGTGLSSHEVAQGYQLVKDPSIYVKVPAADEDF
                     KYLVWTTTPWTLPSNAALCLHPEATYVLVEHEGEKLVLAEALVPRVFGEDKPALKKWR
                     GSEFLGRRYRPLFDFYTDQADTAFVVITGGFVTLEDGTGIVHIAPGFGQDDYEVGLQH
                     GLPVFQAIEPNGIFKAVAGKYAGKFIKDADPEIIRDLKATGALYKKEQYEHNYPFCWR
                     CDSALIYIARQSWYIRTTQFKEQLIRNSNAINWQPDEIRTGRMLNWLENNVDWALSRE
                     RFWGTPLPIWVCDDEKCGRITAVGSIAQLRQEGIDVPAEVDLHKPMMDEVKLRCACGQ
                     TMTRVPEVIDVWFDSGAMPYAQWHYPFENREEFAIKFPAEFISEAVDQTRGWFYSLLA
                     IGTMLFDQSAFKNVLVLEMVLDKDGKKMSKHKGNVVDPFVTVAEYGADPVRWYMVSTS
                     NPWLATKFDLGALQEVVRKYFDTLRNTYSFFALYANIDDVLARAAAAGQTVEAFLADK
                     AGEPTPFDRWITSRYHSLAGEVAAGFDRYDITPAVRKIQNFVIEELSNWYVRNNRRRF
                     WAKGDEPSKMRAYLTLYRVLEGVCRLTAPVAPFLSELIWTELMGPQREKFGIPLSVHM
                     TEFPKADAAAVDAELEAVMGLVERIVSLGRAARARKNLKVRQPLAHMMVQAPPQIPWE
                     RVAEYAPIMRDELNVKDVTPAENIDRFVSYAAKLNFKAAGPKLGPAVKAAAAAVAALA
                     SADVRRLAETGRIELAIEGRRVELGAEEVEVIRSEEAGWAVESEGGTTVALATELTED
                     LIAEGFARELVNKIQNMRKSSGFEVTDRIAVRVASTTRLAEAVRRFGEFIRRETLAEE
                     LEFTVDGHPAEGTEWNINGEPSRIAVRKLKTGTTA"
     gene            45895..46275
                     /locus_tag="PKM94_02165"
     CDS             45895..46275
                     /locus_tag="PKM94_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOD65470.1"
                     /translation="MKKTEKEKLRKLLLAKRKKLVEEMKDTLESNFSTTMTDSDGDIS
                     SYSFHMADQATDAMEREMAFMQASKSGRFLYHLDEALRRLDSKDFGKCIKCGKDIGMA
                     RLEAVPHARLCIACKEAEEHGRRK"
     gene            46259..46798
                     /gene="lspA"
                     /locus_tag="PKM94_02170"
     CDS             46259..46798
                     /gene="lspA"
                     /locus_tag="PKM94_02170"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOD65471.1"
                     /translation="MAAGSSRRLTAPALVILLVAAADQATKFWAVQTLPLREPVAVLG
                     SFVQFTLVYNEGGALGTRLASSSYYLITSLIILGIVLYYLFANRGAARVCYPLAFIAG
                     GALGNIIDRIRIGQVIDFIDIDFFDIHLAGYELERWWTFNIADAAISVSIVYLLASVF
                     LHQPARDPRRLTESGSESA"
     gene            47258..48646
                     /gene="dnaA"
                     /locus_tag="PKM94_02175"
     CDS             47258..48646
                     /gene="dnaA"
                     /locus_tag="PKM94_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOD65472.1"
                     /translation="MTLPQVIPNSPQWKDCLRYIAQRVNEQSFNTWFKPTRGGINGDG
                     QFYLAVPNQFVADWIKGHFGEVIDEAFREVLGSSYQLEFVIAQIPEVPEQNALNLELP
                     LPERPEPLPVPQTQPLGRRHDHNLNRKYTFDSLVVGDFNQFAFAAAQAVAEAPGLNKY
                     NPLFIYGGTGLGKTHIVQAIGNAILEAFPNKQIFYATSEKFTSDFIASISDRTIADFT
                     KVYRDVDVLIIDDIQFFSGKESTQEQFFHTFNSLHHLGKMIVLTADRPPREIKGLEER
                     LLSRFQWGLVADLQAPDLENRTAILYKKLETEGITLPDNIVRYIADQVQTNIRELEGA
                     LIRLLAYASLRQEEVDLKLAEKVLGGGNSVKCKVIEIEAIQRKAAEYFKVEPHMMQAK
                     KKTADVVLARQVAMYLCRSLTSNSLKVIGAAFGGRDHSTVIHACDLISRRMAREPDLR
                     HKIDQISASLLY"
     gene            48933..50045
                     /gene="dnaN"
                     /locus_tag="PKM94_02180"
     CDS             48933..50045
                     /gene="dnaN"
                     /locus_tag="PKM94_02180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOD65473.1"
                     /translation="MKFTLPKSRLGQYLQHILQVVPAKSTLPILSNILLEALENKLKV
                     SATDLDISITASLDATVTKKGAAVVPARILFEIIKELPESEITVETVGSRVELKVPNG
                     SYKIGSVTPEDFPKLPAVNTKKEIRIVAGSLVNMIRKTTFACSDDETRPALNGVLWQT
                     KGEKMQMVATDGHRLAKMATENRQLKGMYEDVIIPPKVLNLIPKFIAGDDQEIGIIFG
                     ENNIIFNLSDIILTSRLLEGPYPNFEQVIPQDNDKRLTIHKDDLAGAVRRVAILSNAL
                     THQVKFSLKDNTLVLSTTNADVSGEGKETLECDYKGDAIELGYNANYITEILSKIDGD
                     EVIFELATSVSAGIVYSPDVAKEDFLCLVMPLRLAD"
     gene            50106..51194
                     /locus_tag="PKM94_02185"
     CDS             50106..51194
                     /locus_tag="PKM94_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOD65474.1"
                     /translation="MILRSLHLQGFRNFAPLAIEFGERVNILYGDNGSGKTNLIEAIF
                     VLCLGRSHRGVPDSVMLQQGTETFRLEGRLSRNGETEAVAVAYQTGGRKKITVEGVPI
                     RPAELYDRFCAVTAGPEDTELLAGSPAARRVFLDIYLAQYSPRYIAQLSDYSRALAQK
                     NAALRMNAAPEAFNAVLVPLGAAITAARARFLEQLVPLAAGHYERISHGSKMTIAYRA
                     SAASVTDSNGAEGMAAQYERKLASYTERERAMQVSLVGPHRDDLAIEIGGYPARTHAS
                     QGEMRTAAIALKLAVYQLLAARRGALPLLLLDEIFAELDDRRIGALLAELAEFQQLFL
                     TTAGQPPSALQAQSRRFEISGGRIAGVQ"
     gene            51194..52240
                     /locus_tag="PKM94_02190"
     CDS             51194..52240
                     /locus_tag="PKM94_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65475.1"
                     /translation="MPELVLREIRKEFDGRSVLEDVSLELAAGELLVLLGPSGCGKST
                     LLRLIAGLEELDRGEILLGERRIDRLPPKDRNVAMVFQNYSLYPHMTVEQNLAFPLKV
                     AKVSRAERMLLVARTAALLGLEDRLKLRPGQLSGGQRQRVALGRAIIREPALFLLDEP
                     LSNLDADLRARMRVEIVGLQKRLGTTMIHVTHDQAEALTMADRIGLLHEGRLVQLGTP
                     EQLYNEPATLFAAQFVGSPRINAVPVTADGEGISVFGFARSELGAAPPAGELTAAVRP
                     EKIRIEPEGRFAASVIGCEYLGDQYVVTLKFGETVLTVAGVTREMRAGEPARFAIAPR
                     DLLFFDRASGRRVG"
     gene            complement(52306..52428)
                     /locus_tag="PKM94_02195"
     CDS             complement(52306..52428)
                     /locus_tag="PKM94_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65476.1"
                     /translation="MFNLPAAAAVLHLFNFPLGAALGVYSFRALLDLPSRSLFR"
     gene            complement(52490..>53371)
                     /locus_tag="PKM94_02200"
     CDS             complement(52490..>53371)
                     /locus_tag="PKM94_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOD65477.1"
                     /translation="PLFPEADYEVAYDDAIAEILDALRPLGPTYLDVLRRGFASRWVD
                     VFETAGKASGAFSWGSYGVHPFVLMNYNATVDNMFTLAHEMGHALHSHLSNTHQPYPK
                     AQYSIFVAEVASTLNEGLLLQHLLSKAQDKTTRLYLLNRYIDNTMGTFFNQVLYAHFE
                     LAIHAQVEGGGALSPEWLTALWADLTRKYYGPDLGDDPYFGLKWCRIPHFYMTFYVYQ
                     YATSYAASESILDNVLAGGPDSINRYLDLLAAGGSDYPIELLRRASVDMSRPAAIEST
                     LTRFADQVAELDRLSQE"
BASE COUNT        10129 a        16589 c        17720 g         8933 t
ORIGIN      
        1 tcctccccga cgtctacgtc ccctgcgagg tctgcaaggg aaagcggtac aaccgcgaga
       61 cgctcgaaat cgcctggaaa gggcgcaaca tcgccgagat cctcgacatg accgtcgacg
      121 aggccctcgc cttcttcgaa aatatccccc gcatcaagaa caagctcctc acgctccgcg
      181 aggtcggcct cggctacatc cacctcggcc agcaggcgac cactctctcc ggcggcgaag
      241 cccagcgggt caaactggcc gccgaactgt ccaaggtcgc caccggccgc accctctaca
      301 tcctcgacga acccaccacc gggctccact tcgaggacat ccgcatgctc ctgggcgtcc
      361 tcaacgagct ggtcgaccgc ggcaacacgg tgatcgtgat cgagcacaac ctcgacgtca
      421 tcaagacggc cgactggatc atcgacatcg gaccggaggg gggcgacgag ggcggacgga
      481 tcatcagcgt cggccccccc gaggtcgtcg cccgcgacaa gcactcctat accggcgtct
      541 tcctcaacgc cgtcctgaac gggaaagcgc tgtaagtcgg acggcctccg gcgccgcccc
      601 gaggtgaacc atgcccgaac tccccgaggt tgaagtcgtc tgccgcggcc tgcgccgcgt
      661 cgtggtcggc cgcaccatcg gccgggtgac cgtccgcgcg cctaaatcct ccgtcaccgt
      721 cagcccctcg ctcggcgcgc ggtttctgga cgaggccctg agaggccggc ggatcgagtc
      781 cgtcgaccgg cgcggcaaga acatcctcat ccgcctcgcc ggccgccgca ccctctgggt
      841 ccacctgaaa atgaccggcc gcttcctctg gctgccccgg accgaaccga tcgccgcgca
      901 cgacctcgtt atcgtcgact tcctccccga agccggcgcc gacgacggac accacctgcg
      961 cttcaacgac taccgccggt tcggccggtg gcggctgtac cccgacgacg agctcgacct
     1021 ccaggaggga ctgcgcgcgc tcggccccga gccgtcggcg ctgtcggcgg acgacttcgc
     1081 cgccctctgc cgccgccgcc cccgccggat caaagccgcc ctcctcgacc agtcgttcct
     1141 cgccgggatc ggcaacatct acgccgacga ggcgctctac gccgcccgcc tccacccgct
     1201 ccgcctgacc accgccgtgt cgcggcgcaa actgcgcgag cttcacaccc acatcgtgcg
     1261 gctcctgcgc cgcgccatcc acctcatggg tacctcggtg gacagctact ccggggtgaa
     1321 cggccggccc ggccgcttcc agagccgcct ccaggtctac gggcgcgagg ggaaaccgtg
     1381 cgggcggtgc ggggcggcga ttgtgcggcg gaagatcggc gcccggtcgg cccacttctg
     1441 cccgcggtgc cagcgggcgg gtgaggccgc cccggggctg ccaagaaaaa acccgccggc
     1501 ctgacgccgg cgggttgtga ctcgcgcttt acgcgacaag tcttactggt gcttcaacat
     1561 cggaccgccg cggaacatgt agccgatcat gtaggtcaga tcagccacgt tcggggtctg
     1621 attcggtgaa gcatcgacgt tcgccacggc cggctccagc ggagcgtcgc cgccgcggaa
     1681 caggtacgaa acgaacatcg tcaggtcagt cacattgacg ttgccgtcga gattcaggtc
     1741 gcccgaccgg tgtccccgca caacaacctg caggttcaac gttcccggca ccccgtcgat
     1801 ctcgtagaac acgtcaaaga acgagtcgat gtcgtcccag atcaggccgc cctggcgcgc
     1861 ctcctcgcag aggacatact ccttcagcga gaacttcacg tccatgacgt cgcccgggat
     1921 ttcgccgaca ccgcccggaa taacctgcgt cacgccaacc gtgaccgggc agccgccgag
     1981 cgccacggtg atgttctcca tggcggccgc ggcgccgtcc ggaagcgcac cgtcgccgat
     2041 gtacacgtcg ccccaggtcg ggttcaacgc gaatttctcg agcacgagaa tcgggtctgg
     2101 cgccaccatg aatgtcggcg cagcgccgcc gacgttcagc gtgtagtcct cgacctcgcc
     2161 atagctcgtc gtcccacacg gcgcgagcgt cccggtgtac atgatgcgga cacgcatcgt
     2221 cacaccgccc tcaacggccc cttccggaac caccaccgtg ccggtgtacg gaccgacgcc
     2281 ggtggaaaca tccagagtga ccatctcgga ggcgtcaaag accacgtcgt cgttccagtc
     2341 gatccagacg ccgcactggt cggacgagta cccgctgccg ttggtgacgg tgatcgcgta
     2401 gctgccgccc acttcgacgt cggtcgagat ggcggtgtag tcgccgtact cggtgcaatc
     2461 ggacgaattg tcgatcgtcc cgatctgcac ctgcgagatg tactcatcgc atccgccgct
     2521 ggccgcgcac ggagtcggct cggccggcac ggaggtcgcc gtcagcgcgt actcccacgg
     2581 acccatcgag cacgggaagc cggtgtacgt ctggccggag acgaagatga ggtaatcgcc
     2641 gggggcgagc gtgagcggct caagagcgct cggctcgcaa tcgcccaccg cgaggtacgg
     2701 cgcaatgtac ccggtgatgt tgtcgcacga ggcaatgccc ggcgtgatct gctcgatcca
     2761 gccgaacacc gcatcgaagt tcgtctcgat gctcagcgtc acgctgtacc agtcatccag
     2821 cgacaggatg taccagtcgg tgtcgcggta ctgcgccgac ccgtcgggcg tgtacgtgct
     2881 gaacgtgccg cagatcttct cgttcggacc gatcgatccg aacaccggca catccatatt
     2941 gcacccgccg ttggttacat cgtcggcgta gtcctcaatg cagtcctcac cttccggcgt
     3001 gccgcccgtg caggtgaatt cgcagggcgg agccagcgtg atgttcagaa tgccgttgcc
     3061 gacattcgtc gaataaccgc cgacctcgac gtagtaggtc atggccggat ccaccggtat
     3121 gtcgtagagg accgactgca gacccggatc gcaagcatcg tcgtcgcatc cgatctgtgt
     3181 cagcgcaccg cacgtgccgg tgtagacccc cagcctcgtg tcgtattccg acccgcagag
     3241 gctgatcgtg gcaagaccgg ccgccgacgg cttgtaggcg aaccagatgt tcggcgacgt
     3301 catacagccc gtcggaccat cgtgcgatgt ccccacggtc gagaacggca ggtcggtcac
     3361 ctcgcccacc tcggtggcat cctcgcacag gtcattctcg atcgccgcgc acgtcgtgtc
     3421 ggtgattgtc agaatgaacg acgggataca gttgggcgac ggccaggtgt cgacgatcag
     3481 gtagtacgtc gaacccgccg tcagattgac gcaggtcagg cgcttgaccg tcgtggccga
     3541 accggtgacg aacccgatgc aggtcgtgaa cgcgcagtcg cccgccaggg cgaaaccggt
     3601 gtaggtcgtg ccctgcggat ccatcgcgat gttcaccgtg acatcagcca gcgcggtgat
     3661 ctcatagatg atgtcctcac cgccgtcata cgacccgagg caggtgttgg cgtagtcgtt
     3721 gatgcggccg caggtggtgt tggtgtccac aaacggcaga accgggatat caaccttcac
     3781 cgggtttgcg cagttgtcgc ccggattggg cggcggcgct tcctcgaaga tgatgtcgag
     3841 ttcggggata cagtcggggg tcggccaggt gtcgaccatg atgtagtagc tgcccggatc
     3901 cagctggacg ttgtagatcg tcttcgccgt tccgccgccc gtgcccacaa accccaggca
     3961 ggtcgcaaag gtgcaatcct gcgaaatgcc catgcccgac cacgtcgtcc ccttcggatc
     4021 cagcgtgatg ttcacgaaca tggcgtcggc cacgtcgacc cgaacgatca ggtcttcgcc
     4081 gccgtcgtag tagcccatgc acacggtcgc atcgtaatca ttgatgcggc cgcacgtgta
     4141 ctggcctgcg acggtgtacg gcaggtccgc cgaactcagc gcgaccacga acgggtcttc
     4201 gcagttgtcg ccgggggtga ccggcggaca ggcttcggcc gtcacgtcga cgacgtagtc
     4261 gccgatcgcg ccgttcgtcg gatcaagcat gatcgggtag taataggtgc cggccgccag
     4321 gtgactgaag tagatgttgg cattgccgtt agggcagccg aagtcatagg tcgcgccgct
     4381 gatcagcgag ccctccggac aggcacagtc ggtgaccagg ttcagccagc cgttgagcca
     4441 acccgaggcc gtgttgcagt agctgatcgt gatatccgag cattccgtga tcgtgaacgc
     4501 gatccagacg ttcgggtagt cgccgaaata ctggcagtag tcgtcgaacg tcgccgcctc
     4561 gttgttgccc atgaacgtat acggcagcgt catcggcgtg acttccgcgc agttgtcgtt
     4621 cgggccggcc accgggcacg gattgtccgt gcagttcaaa ccggcgctcc agttgccgcc
     4681 taacgccgtg cactcgactt cggtgacatc ctggcagttc ggagctgtcg gctccccgta
     4741 gcagcaccga ccggccggcg gagcatcgtc cgtcacgacc acattgtcca gaccgaacag
     4801 gtccgcatcc atcccgtaat actggaatcc cagccaaatc ggggcgccgc cggcgtaagc
     4861 cgacagatcc accgtcacgc cgtaccacgt gaaggtcggg tacgcaacgt cgtaggaatc
     4921 ccagatttcg gtgaacgccg tgccgtcgaa cgacacgtag accttgaagt cgtgcgggta
     4981 gtcatacccc ttcaacatgt cgaattccag ccgcagcagg ctgccggcgc ccgacacgtc
     5041 catcatcggc gtgatcaacc actcgtcctg ctggtagccg gcggcatgcc aggtcaccag
     5101 cgcgcactgc gtacctccgc ctgagtacgt cccgagaaac cagccgtatc cgggatccgt
     5161 gttgatggaa tcccagccgg tcggcgggaa gatctcggtt tcaaagtctt cctgcaggaa
     5221 gatggtgcgc ggctcgtaat tgaccgcggg ccgcaggatc gtgttcgggc ccgccatccg
     5281 gttacgatcg gactcgcgcg acggtctcgt cggacggacc ggctcttcgc ccttcgcctg
     5341 gtccatcgcc gcgctctcag ccgcttcccg agccgtcagg tcggcttcca tctgggcctt
     5401 ctcgacctca aacggatcga gcgccgccgg ccgggccgcg ttgattgccg ctttcagatt
     5461 gaccggatcc gccgcccgcc gctctttgtc gagatcacgt ggattaccaa tcccggagag
     5521 ggccaccgcc ccaaggacga gaacggcgac gagggcgacg aaaacagggc tatactttcc
     5581 ccatttgatc atagctatac ctcttgattg ctggacggtt aatgtgtgat gcctcggcta
     5641 gcacgagcac cacaacggtt ccagaggccg gaaagtgtta ctgatagaca ccacctcctg
     5701 aataggggtt gacggttgcg gactgaatcg tctcttttat cccagacaga cagctatgtt
     5761 cttctcgtgc gaacataaca ccggacaagt actatatata ccccgcttgc catatgggcg
     5821 agtaaccctg agaagatagt gggccgctct caccggtcgg cggaatgtat cgtggttgct
     5881 gttaacaaat tagcccctga tccccgctaa gtcaagactt ttttcccgca aacttcccat
     5941 tcactcccgg tcagagatac gtagacccgc ttaccgataa acctccccgt cgctccactg
     6001 ccgatggcag ctgcgcccgc cgccgtgagc aatcccccgg cccgcccgaa tcggagtccg
     6061 aagctccgca agtccgctgc actttccgcc aattgtgtat gcaatgtgtt gcaggtagag
     6121 gtcgccagaa taaccggcgc ccgtctccct tttgcgaccc gagtccccaa tcgatctccc
     6181 ccatttacgc cgtatcacgc agtgcgggcg aatgtaaagc gcgcttcttc atactcgaaa
     6241 gcggtctcga aaccgagctt ctcataacat cggcgcgccg gcaggttgtc gaccatcacg
     6301 ttcaggcaca ccagaagctg ctcgtctgcc agttcctgtg ccagtctggc tgtgaggcgg
     6361 gaggcaatgc cctggttgcg ctcactgggg tcggtggcga cgttgccgat caccgcgacc
     6421 ccgtggtcgg gacaatagac gtgcacgccg gccgcgccca ccagccgacc gtcgacgctc
     6481 cacccgaaat acttgcccgt cgcgaccatc gtctcgtcga accggcaatc gggatacgcc
     6541 tcgcggtaga accgttgcag atcggctgca tcggcggcgc ccagccgacg gatcgagtgc
     6601 tcgtcccccg acggcccgct ccgcagcgcc gtcagacgca tccggattcc cctccccacc
     6661 ggctcctcgt cgtaccggcg ggcgaacacc ggccggcagg aggacgcgaa gtgcgcggtg
     6721 aaacgcggcg gcagcaggtc gagcgcttcc gccaggagct gctccagccg ctcgctcagt
     6781 ccgaacgcca gcacggtcgg gccggacggg gcgcggtaga tcaggatgca ctcgtcgatg
     6841 cgcgtgctct tgtggtggct caccgcccac tggcagcgcg aaaacagcgc gtcgtccaga
     6901 tcgcccaggt ggtaggcaaa acgcaccggg tcgcggcgga agtgctccgc gaggcgcagc
     6961 ttgtcgtgcg tgaacagcag catcggcgga cggcgacggg cggcgccgca tcagcccccg
     7021 gctttgatct tctgcagcgc gtcgatcacc tcgtcgagtt gatcgcggcg gaaagaaact
     7081 cccttcttgg tcggcagcca ctggctgtcg tcggccagga agtaaatacg gagctgcacc
     7141 aggtggtgtc ccttgtactc gctgtcttcg atcaccaccc gttcggtcgc attcttatcg
     7201 agctcgaatc gcatgtttgt cgtcctgtcc gcccgcccgc cgtggcccgg aacggggccg
     7261 ccgttaccgt ttgtagccga tccggcggag aaactcccgc cgctcagccc tgtctgcttc
     7321 cgtttcgatc cccagcgggg gctgcccgtc gatcaccccg agcacgccgc gcccgagatc
     7381 ggtctcggcc accagcactt cgagcgggtt ggcggtcgca cagaagaccc ggcacacttc
     7441 gctggtgctc ttcacccggt tgagcacgtt gaccgggaaa ccgtccttga ggtaaatgaa
     7501 aaaggtgtgg ccgcagccga cgtcgaacgc cgccttcgcc gcccgctcga tcaggtcccc
     7561 cgcgttcccc tcggtccgga tcgcccgctt ctgggacgcc tcgcagaagg ccaccccgaa
     7621 tttgatcgtg ggcgaggcct cgatcagcgc ttcgtagaga tcctcggctg tcttgatgaa
     7681 gtgcgccatc ccgaaaatca cgttgagtcc ctccgggatc tcgaccttca ccacgtgggt
     7741 cttgaacttc tcactcgcca ttgtccgcgt cctccgattt gaacagcgtg tcggactggc
     7801 cgacgttgat ctggggattc cggtagtatt cagtgccggc gaaattgact ccgaaaaaca
     7861 gggccgcttt gccccgcccc gccacgtcga actgcttcgg cagccagaat ccctccggcg
     7921 ccggaccata gacgatcgac atgtccagac gatccagctt gaacatcgct ttcttcacca
     7981 gccgggccgg ggagaaatcc acccgcacca ggtgaaaccc gtcggtctcg aaatagtagt
     8041 ccccggcgat gtgctcgtcc gtcttgacct tcggcgtcac ccgaaaatgg tagcaggtcg
     8101 tgtcctcgac ttcgtcgagg ccgacataag cgatctcgta atccttccgg ttctcgggcc
     8161 ggaacgggcg gagcatcgga taggcgatgt tgcgggcgtt gcgcttgcgg ttcttctcca
     8221 accggtcgcg cgcctgcttg gcggtctcct ccggcgactg ctgcaccccg tcccggtagt
     8281 actcgcggta ctcctcgcgg tacagggcgg tgtccttcgc gaacttgatg aagactcgct
     8341 tgaggaaacg ggcctccgcc tggaactccc cctcgctgat ccggccctcg tagtactccg
     8401 catccagtac gagatcgtga agctgggccc gctggcggcc gtcgaccgcc aggaactgct
     8461 caatcagagc ctccttatcg atcagcggcg attccggcgg agccgccgag aggccggccg
     8521 cggtcaccaa cagtgcgagc agcgcgccac gcgtggcgtt catggtgcgt cctttacgat
     8581 tcgtacagtc ctgagcgtat cgccgatcgt tatccgatcg gccgtctcca tcccctcccg
     8641 cacccggccg aagagcgtgt agcgcccctc gaggtgcggc tgcggcgaga gcgtgatgaa
     8701 aaactgcgag cccccggtgt ccttgcccga ggtcgcgatg ccgaccgccc cgcgctcaaa
     8761 ggtttccgcg gagtactcgt cccggatata gtacccggga ccgccccagc cgtcaccccg
     8821 cgggtcgccg ccctggacga cgaaattcgg caccacgcgg tggaagatca ggccgtcgta
     8881 aaaccccgcc tccgccagtt caatgaaatt catcaccgtc aacggcgcga cgtcaaagcg
     8941 cagctcgatc gccaggttcc cccggctggt gctgatcagg gcccgcggat tggccgcgta
     9001 tccggcgagc gcccgcgcga tcgcccgttc gctcagcctt gtgtcggcca gcagcgacac
     9061 cgccggcttc ggcgccgtca cgagcgagtc ccacacctgc gaagcatccc gccggacagc
     9121 cgcctccgca tcgcgaaggg cgagccgcag gagttcccgg gccgccgaat ccgcccggga
     9181 ggacaaaaac ggcgggagcg cgccgaccac cgaccgccgc acgtccgccg cggtccccgc
     9241 cgccggctcc atcagcgtgc ggaacagagg caggtactgc ggatctcccc gcttccccgc
     9301 ggcctcgacg ccgagcgcct gcaggacgaa atcgctgtcg tggagcgcct ggtcgatata
     9361 gaaatcgccg ttggcgctgt cggtctccat gagcattccg aacgcggccg cccggacctg
     9421 cggcgaccga tccgccagca gcgtggtcag gcgcggcagc actgtccgct cgcggatcag
     9481 ccccagcgct tcggccgccg cggcccgctc gaacggactg tcgccgcgca gcagcgaatc
     9541 gatcaggtcc atcgcccgcc ccccgcgcgc ggcggcggcg tacttcaccg tcgcggccgc
     9601 gatcgacggc gtcgcgtagg ccgccagcgc ctcctccgcc gtcgcgattc cctcgctgct
     9661 gcccagccgg cggagagcct cgatgagttc gacgatgagc ttgggatcct cctccaccgc
     9721 gagcttcttc tggagcaggc gactgacccg gtcggggtcg cgcccggcca gttcggcgat
     9781 cgcctgcgcc accacccggt ccgtattgtc gttcagggcg atgctcacgt agcgctcggc
     9841 ctcttcgccg gcgaccgcgc ccatcccgcg caccgccagc ccccgcaggt tcgggtcggg
     9901 gtcggcgagg taggacgcga agatctcctt gccggcggca atgtcgaagc gggccaggaa
     9961 gtacaggcag gcttgccgca ccggctcgtc gaaatcttcc ccatacaggc cgatcacctc
    10021 ggccgcagcc gaccgggccc ccgcccgaaa gagcccccgc acggcccgct cccggacctc
    10081 gggcgcggga tgcgacaggt aggccagcag cgcatcgatc acctccgcct tgccggtgtc
    10141 ggccagccgc ccggccgact cgaccgcccg ggctgtgatc cgcgggggca agttgtcggc
    10201 cgtttccaac aggcgaagag cgaactccgg ctgtccgcag aatccgatcg cggtggccgc
    10261 cgcccccgcc acctcccaac tgctgtcggc cagcaggtcc aggagcggct ccgccgatcc
    10321 cggcccgccg atgcggccga ccgccagggc cgcccgcgcg cgcaccgccg ggtcgctgtc
    10381 gcccaagtag ctcttcagcc gccccgacag ctcgcggcgg tcttccaggt ggatgatctg
    10441 cgccaatttg tcgatgcggg tgggcggggt gagccggtcg taggcgtagt acccggccac
    10501 cgccagcagc acggcgatcg cccccagccg gagcctcttc acattgtcgt tcatagaggg
    10561 agaaggtacg gccgggcttc ccccccgtca atcgtttaga caccagccac gtgtgccgcc
    10621 cggcagcgcg gccgcggttc gccggcgttc cgccctcccg cgcttgtccc cttgcccgca
    10681 gcgccccccg gccgtattat gcagcatgta caacccgacg gtcatggacc acttccagaa
    10741 tccccgcaac accggcgacc tcgaccgccc cgacggcatc gggcaggtcg gcaacccggc
    10801 gtgcggcgac gtcatgcgca tcttcatcag agtcgaggcc ggccgcatcg ccgacatccg
    10861 ctcccagacg ttcggctgcg ccgcggcgat cgcctgcagc tcgatggcgt ccgagatggt
    10921 gcttgggaaa acgctcgacg aggccttcgc gctgacgcgc gacgacgtcg cccaggcgct
    10981 cggcgggctg ccggaaaaga aaatggcgtg cagcaacctc gcccccgatg cgatccgggc
    11041 ggccattgag gactaccgcg cgcggcggtg agcgcgccgc cccgcccccg gctacccctt
    11101 gaacttccgc gccgcctgct ccagcttctc atccgtgagc cgggccaggt cggcgccgag
    11161 ctcgtcgatc tgcgcctcgc tgagccgggt gagccgcgag acgaattcgc cggaggcaat
    11221 acggtcgtac acctgctgca tgcgggcctt caccacctcg tcgatcagaa acggaccggt
    11281 ctcgagcgag ccgtagcggg cggtcagcga gatgcgctcg aacatccccc tgatcccgtg
    11341 cttcttgatg agcgcgacaa tcagatcgag ctggtaggcc acttcgaggt aggcggcgtc
    11401 gggcggaatg ccgtttttca ccagcacctc gaatccgccg gtgatcagcg cggccagccc
    11461 cccgcacagg accgcctgct cgccgaacat gtcgccgacc gcctcggcct cgaacgtcgt
    11521 cggtaccagg cgggtcaggt caaaccccat ccccgccgcc aacccgaaca tagtggggcc
    11581 ggcctggccg gaactgtccc ggtggaccga gacgaaagcc gacatgctgc ggtcgccggc
    11641 gaggtagttc gcccgcaccg ccactcccgg cccgtgcggg gcgatcagga tcacgtcggc
    11701 gtcctccggc ggttccacca gaccgaaatg caccgagagg ccgtggagaa agaccagggc
    11761 cgcgccgggg cgcaggcggt cgcggatctc gcggcgatac acgacgccgt ggtcgggaaa
    11821 ggcgaaaacc acgccccccg cttgcccgac cgccgccccg atcccgtcga tccggggcat
    11881 cccgtcgcgg cgtgcatccg cgcgccgggg ggagtcgggg cggagaccca caatgacgtc
    11941 aaagccggag tcgcgaaggt tctgggccac cgcgtgaccc tgagatccat agccgatgac
    12001 ggccaccggg ccgccggtcg aaagatcctt catggcgcca atatagcccg ccgtttgatc
    12061 gttgacaatt gcccgccgcc atattgtttt cccagtatgc gcgatcaatc gctctggctc
    12121 gtcgaactgg tgaaagcggc cctgggcgag gatgtcgggc cgggggacct cacctccatg
    12181 gggtgcctcg agcccaatcc tgccaaggcc aggatcgtcg ccaagcagga cggcgtcctg
    12241 tcggggctcc gccccatgct cctggtcttc ggcatcgtcg actccgccaa cacggtccgc
    12301 gtgttcaaaa acgacggcga ccggtttgcc aagggggaga cgctggtcga aatcgagggc
    12361 ttcaaccaat ccatgctcgc cggcgaacgg gtggccctca actttctgtc gcacctgagc
    12421 gggatcgcga ccctgaccgc caaattcgtc gccgccgtcg cgggtacggg cgcccgtatt
    12481 ctggacacgc gcaagaccac gccggggatg cgggtcctgg aaaagcaggc ggtggcgcac
    12541 ggcggcggct gcaaccaccg catgggcctc catgacatgg tcctcatcaa ggacaaccac
    12601 atcgcctcgg ccggctcgat cacggcggcg gtcaaccgca tgcgcgactt cctcctcagc
    12661 cccgacttcc gggcccagtt ccgacgcccg gcccgcgaaa tcgagctcga ggtcgaagtc
    12721 gtcaacgaga gacaggtggc cgaagcgatc gggctggggg tcaaacgcct cctgttggat
    12781 aaccagtcgg tcgactcgct gagagcgctg gtggcgaaag cccgggcgat ggaccccgag
    12841 gtcaaactgg aagcctcggg caacgtcgcg ctcgacaccg tgcgggccat cgccgaaacc
    12901 ggcgtggatt acatctccat cggccagctc acccactcgg ccccggtcgc cgacctgagc
    12961 atgcaaattg tcgagcagcc tctcgatgaa ccggcgtgag gtggagcggc tggccgaccg
    13021 gctgcttctg ctcatccgca ggcggccggg ggggacagtc gggatggcgc agctaaccga
    13081 ggagttagga gcgccccgga ccgaactgga ggcggccatc gcccaactcg aaacctgggg
    13141 ctacaccttc aagaccgcca ggaatcgtct gcacctgacc gcaacgccgg acagcctcac
    13201 cgccaccgaa ctaaagtggg ggttgaacac cgggttcatc ggacaacggt tgttggcgta
    13261 caacaaggtc aagtccacta atgacatagt atcgcgagaa ggggccgcgg ggggggtcga
    13321 agggaccgtc gcggtggccg agcaacagac cggggggcgg gggcgtctgg ggcggggatg
    13381 gcactcaccc aaacgcaaag gcatctacct ctcaatcttg ttgcggccag aatttctgcc
    13441 tgagcacgct ccgggtcttt cgcttatgac ggcgctggcg ctcgcgaaga cgatcgaagc
    13501 gacagaaaag atacgaatca agataaaatg gcccaacgac ttaatattga acgaccgaaa
    13561 ggttgcggga atcctcacag aactcgcggc agaacgtcgc agaattaatt atgttgttat
    13621 cggcgtgggc attaacgcca atcaagacga gcaggatttc cccgaagagc tgagacaatc
    13681 cgcaacctcg atccggatgg cggtcggaca cgaaatctcc cgcgtccggc tggtgcagaa
    13741 cttcttccgc gaatttgagc gggaatacca actctactgt cgggaattcc tcgcgccatc
    13801 gctgccgcgc ttgcgccggt attcgaccct cctgggcgaa aaagtgcggt tggcgtcggg
    13861 actccagata ctcgaaggag tggctgtgga catcgagccg ggcggggggc tggtgatcga
    13921 gcggggggga aagcgccacg tggtcacggc cggggatgtg acggtggtaa aggagtaggg
    13981 gggaggcgac gcccgtcggg accgcggccg gcggggagat gatggtgggg aggggggtcg
    14041 gggatgggtt ccccgggccg gcggagggga gatccccaat cgacttgacg gaccgattta
    14101 gtctattatt tcgcttgacg gcggcgcccg gcgtgttata tttccgcccg ttccaccact
    14161 gcggacttac cggaaacact gctaacagag gagcaacctt gaatcagcga gcaattctcg
    14221 tgggccgcgg ccggttggtg gccgcgggcg tggtcatggt gctggcgatg cccctcgcct
    14281 ggctgtgggc ccagggcgcg ccgacctaca cggccgagca ggtcaagcgg ggggagtatc
    14341 tcgtggtgat cggcgggtgt catgactgcc acacgccgaa gaagatgacg gagcgcggtc
    14401 ccgagcccga tttcgcccgg tcgctgagcg gccacccggc ccaggagaaa ctgccggcgg
    14461 tgccggcggg cgcgatcacg ccgaccggct ggatggcgct gacgaacggg aacctcaccg
    14521 cctgggcggg gccgtggggg gtcagtttcg ccctcaacct cacgcccgac gtgaagacgg
    14581 ggatcggcgg gtggaccgag gagatgttca tccaggcgct gcggaccggc aagcacctgg
    14641 gcatgggccg gccgattctc ccgcccatgc cttggcagaa cctctcccac gccaccaccg
    14701 aggacctgcg ggcgatcttc gcctacctga aatcgctccc ggtgatcgag aaccgggtcc
    14761 ccgatccgat tccgccggcc gtgcattgag atatcccgga gcggaccacc gcgccggaaa
    14821 gcccaaatcg acggcgggag ccgatgctcc cgccgtttcc tttgctcgag ctcctcaagc
    14881 gacaacggcg ggtgctccga cttgcaggcc agcacgccgt cggggatcta cgccccccgg
    14941 aaaaagcgcc gggaccgccg cccgtagatc gcctcgagaa gggggaactg gagagtgacc
    15001 tcgaggcccg ccgggatgga ggcgccgccc ccgcgcgcca ggattcgtcc ccgggcgcgc
    15061 gctttttccc ccgccgatct caggccgcag ccggagagca ccgcgccgcc ggccaccagc
    15121 aacaggccga ggagccgccg ccggcccaga gaactcaaca agttgcggga accgtgcgtc
    15181 atgcgtcctt cctccgtgtg gggatgtgcg ggaccgggcc ggcaaaggcg cgggaaggcc
    15241 gcccgcccgc cgcccggtcg gaccaaccgg tttttttgcg gctcttgacg aaagtcaatt
    15301 tccgccgaac cctgtcctgc gtactgtccg gggcggttct ccgcgcccgg agcggcggcg
    15361 ggaaccgccg tgccgacgtt tgcagcggga ataactcatt ttctaccaga ccaactcgcg
    15421 gtcggccgca atggggcggc cgccgcaaac gtgatgtgag aggagtaacg tatgaggagg
    15481 ttggttatga cgggactttc agcggtggcg ctggcggtgg cgggaatggc cgccattgcc
    15541 gggaccgagg cggcgccgcc gggggccgcg cttctgccgc cgtcgctgaa caagctctat
    15601 cccccggagg cccccgcgcc ggtgtacctg atcgagatgc acgcgctggc ggcgccgctg
    15661 tcggggatga tgtccgacct gatggaaggg gatcacgcga acgcgatggc caatttcgag
    15721 gccttccgcg cccagtatgc aaaggtggcc gagctcgtcc cggagtggcg gcacgagttc
    15781 ccgacggggc cggtggagac gctggcgaca gcgctccgca gcggcgacca gggagcggtg
    15841 atgcgggccg gtgacgcggt cggccgcgcc tgccacggct gccacctcca gactatggtg
    15901 cccgcccagc accgcttcca ctggggagac ttcggcgcga tctcggtcac cgatccggtg
    15961 atcggtcagg atatgccgtt cgcccagttc atgcatatgc tcgacgggga tctcgcgggg
    16021 atcggcaatg atctgaagca ggggcagccg gagaacgccc gtcggcatgc cgagggactg
    16081 gccgcccggt atgccacgct cgaagaagtg tgcggcgtgt gccacgacac cgatcggaag
    16141 tactacgtcg acgagaaggt gatgcagatg attgcggacc tccccgcggc cctcgagaac
    16201 ggcaaacctg agaacctggg cgcgctgctg cagggaatcg ggcaggaaag ctgccagaag
    16261 tgccacctcg tacacatccc ggcggcgtac gcccgggcgg cgcactgagg cgacacggtt
    16321 cggccgggtg gcgcggccgc tccccgcggg gaagccatcc ggccgagacc gaaattcgac
    16381 tcaagcggcg aaagtcagcg ggccgacgcc ggccaagccg caagaaaagt gatgtcgtcc
    16441 gtctggctcc ggtccccgag gaaccggcgg acaccgtcct gaaggtgctg cggcagcgcg
    16501 tcgcgacacg cccaccccgt tcgggcgagc gcgtacagcc gctccaggcc gaacatctca
    16561 tcgtcgctcc aggcctccgc ggcaccatcg gtgaacaaca gcagcagcga atcggcgggc
    16621 aacgggatcg tcgccgtccg cagagccgac gggggtacca ccggtgaaat cagcggagac
    16681 gtcacctcga gcgggagtag ggtctcgtcg ccgccgacga tgaggccgcc ggaatgaccg
    16741 gcgttgacat actcgatcgt ccgtccggac cgggagagcc gggcgcacag cagggtcacg
    16801 tattgatccg attcgaacgc ggagaggtcg tccaggagga tccgcgccac ccgcagaggc
    16861 gcatagccgc ggtccgcggc gcggtggaaa gcggatttca ccagcgccgt gaacatggcc
    16921 gccgacgcgc cgtgcccgac cacatcggcc accaggagag tgacaagatc ccccccggtg
    16981 acggcgtagt catagtagtc gccggcgacc tggtcgcagg ggtagtagcc ggcgcgcagc
    17041 tcaacgccgt tgaggtgggc cgaagcgggc gggagcatgg tcctctggaa ggcgccggcc
    17101 gcgagcaact gccgccgcag gtgatcggca tacgcccggt tgctcagttc gagccgcttg
    17161 agttcaaggc aacggtccac cagcgccagg agcgcatccc gatcgaacgg cttggtgatg
    17221 tagtagaagg cccggttgcg caccgcccga acgagcttct cgtccgtgtc gtccatgctg
    17281 ccggtcatca ggatgacctc gagatcgggg tcgtcctcct tgagctgagt catcaggtcg
    17341 aaaccgtcga ggtggggcat gcgaatgtcg atcagggcca ggtgcggacg gctgcggcgg
    17401 gcgagatccc gggcggccgg cgaggaatcg cacagagtca ggtcgtgggc gctcccgaga
    17461 acccgctgca ccgcccgcag cacccccggc tcgtcatcga ccaccaggat ccgccagcgt
    17521 ttcatgggag gcgctcctgg cggcgggacg actgggtggg gagccgcagg gttacacagg
    17581 tcccctgacc gacggtgctg tcgatgcgga gttcgccccc gatgttccac agaatccccc
    17641 tgcagaccgc caggccgagg ccgtgcccgg actccttggt cgtgatgaag gcgtccgcga
    17701 cccggtcgag gaggtcgggc gggatgccgc cgccgctatc ctccacgcgc gcctcgatct
    17761 cgggaccctt ttcgacagcg ctgacggcga gccgcccccc gtgcggcatg gcctcaatgc
    17821 tgttggcgaa caagttgagg aacacctggg tgatgtgccc ctggttggcg ccgatggccg
    17881 agagctccgg ggggatgttc agttcgacgg cgatgctgtg gcgatcgagg cggctgcgca
    17941 cgacgttgag ggcgccgtcg accgcgcggc ggagattcgc ctccccgatc gtgcggtcgc
    18001 cggagcgggc caccgagagc agccccgcga aaatgcgccg gcaggcgagc atgcccgatt
    18061 cgatcacatc gagatcggcg agcagctcgc gctgatcgag ttcgccgcgg gcgatgtcgg
    18121 cgcggatctg ctgggccagc ggcagcaccg agccgatggc attgttgacg tcgtgcgcga
    18181 tccccctcga caggtccgcc agcgcgctct tgcgctccgc ctgtaccacc tgctcggtga
    18241 gtacctcggt gcggtggtgg aactgaaggg cgagcgacag cagcggcgtg aagttctcca
    18301 ccagctcgat ctcatacgcg ccgggaatat cggcccgctg aaaagcaatc ttgagaatcc
    18361 ccagcgaacc gtcgggagcc gggatcacgg cgcagagcac cgcgttctct tcgggcgcgc
    18421 tcgcgccccg cggacaggtg cagcagagcg cggggatcag ccccggcgtc cggacggcgc
    18481 cgaacaccgg ctgccagccg tggcccccct ggcggtggag gtggatgccg ccgcggctta
    18541 gctcgtcgcg gagctctgcc gccagcggta ccgtcagccc gatgcggcgg ctctttgctt
    18601 tcgtccaggc aatctgttcg gcgatgatct ggagcggacc gtcgggaccg ttggtggcca
    18661 ggaacgtggc cgagtggtcg tagcgggtca gcgagcggat gccgtggagg atgaggtaga
    18721 tgatgtcttt gggttcccgg cggttgacga ctttctgttc gaggcgatgg cgcaggcggc
    18781 ggatctgccg gtcgtcatac tgcgcgatcc cttcccggac cgcctcggcg atgagaccga
    18841 gcgcgcggag gtggaaggcc tccttgggat ggggcaggcg cacggcgata gcgccccagg
    18901 tcgccgcccg ccgctcgacc cgggccagca ccacgtcgtc cggaatgcgc ggctcccgcc
    18961 gcttgatgaa atcgttgagc aggccgacgt tccattcggt cgaggtgcgc gtggtgaaga
    19021 gcaggtcggc cgtgtgggcg gacgcgctca acgtcgccac cgccccggcg tcggccgcga
    19081 gaaactccac cgcccggcgc atgccgtaga tgagcatctt gcggggctcg ccgtaatcgc
    19141 gtagcgtgcc gcggagatcg aaaagtacct tgtacaggtc ggcgtgcgtc ttgtcgatag
    19201 tgccgtcgaa catgacaact cctgcgtgcg cggtgcggcc cccgctcgcc ggcggaggca
    19261 taacacgtgg gcgccctgcc aagcgcggtc gaccccatgg agtcggcggc tggtcgcagt
    19321 atacggaacg gggaccgaag tgtcaacggt ttcggggaag cggggcggcg aaaagccgcc
    19381 caaacgagag ccgggcgccg gatccgccgg gacgcacccc gcgcgcggcg aggcgcggcc
    19441 cggcggccga gccgtggtcc ggttacgccc gcaggcgagc ggggcggctc agcgccacgc
    19501 cccgacgatc agccccatca ggagaaaacc cacgatcgag tagagagcgt tcaccacagt
    19561 cagcgaacag gggcgcttct ccatgacgtg gtgcatggcg accgtcgcgg ccgcaaaaca
    19621 gaccccggcc aaaaacccga ccagaagacc gtcgcccgcc gacgtcccgc cgctccactg
    19681 cacgagtcta gcaaccccgt aggcggcgac gaacgagaag atgaacgtcc agaccaggtt
    19741 ccagggggag aaatccgcct tcacctgttc gtcggtcttg ccgatgccgg tccgccagac
    19801 cttgccgaac acgggcggag cgtaccagag cgcgccgagg acaaaatagg cggccgcggc
    19861 gaccaggatg gcccagtagt tcagggcctg ggggaccaga acttgcgctt ccacgcagca
    19921 cctcgcttgt aactagtgtg agggtcgatg gaccgcccta cgttggccga acgtgcggga
    19981 ccggcgcaag ttaaaaatgg agtgcggcgg tcctaccgga acagcagcgt ggtcacgatg
    20041 atctgggcca gcaggatctt ggcgatcatc gaggcgggct gcaccgtggc gtaccagagg
    20101 ttgaccagct cattctgggc ctgctgattc gcataggcga gcacggccgg ctgggtctgg
    20161 atccccgcta ccagtccgaa ggtcgccgac atgggcagct tcaggtagcg gtagaaggct
    20221 atgatggtgg cggcggcgac gaacgaaacg atgagcgcgg cggagacaat catccccagc
    20281 gccccggtct ccacggtggc ggtcagcccc acgccggcct tggtgccgat cgcggcgagg
    20341 aagaagacca gcccgatctg ccgcagcgcg aggttggcat tgaacgggag gctccagtgg
    20401 atcggtccgg tgcggccgag gcggccgaga accaggccgg ccaccagcgg cccgcccgca
    20461 aagccgagct tgaacgccgt cccattgggc agcggaatcg gaatcatgcc gagaaacacc
    20521 cccaggacga tccccgccga gagcgacagg taatcggtct cggagagcgc tttgacggaa
    20581 tcgccgaaat agccggcgat gcggtcgacc tcgaggcgcg gcgacacgac cagcacgcgg
    20641 tcgcccatct ccagcgttgt ggtcggggac gggacaaact cgacgtcgcc ccggcgcagg
    20701 cgcgtgaccg tcccgacata ggcgcgctgg cgcctgagct cgctgatcga ccggcccacc
    20761 gccaagggat tcgagacgaa gaaccacttg tacgccgtct catcccgccg ttccgccagc
    20821 gtctcgttcg attcgctgcc gagcaggagg tgggcagtgc gaagggcttt cgaggggccg
    20881 acggcgacga tgaggtcgtt ggcctcgagc acggtctccg ggctcaccac ttgcaccagg
    20941 tcccccttct tgatgcggct gaaaacaaac tgcggctgtt cgaaagcggc gaagaactgc
    21001 tcgacggtct gtccctggac ggcgggattg gtgacccgga acgtccggct cgcgatcgct
    21061 tccgcatccg cctcgcgctt gacttcctcg gtctcgcgcg agaaatccac gcggtaaatc
    21121 ttcgcaaacg cgaacatcca gagaatcatc ccggcaacgc cgatagggta ggccagaccg
    21181 taggtcacga ccgggctgtt gaggtagaac tggactttgt ccgggggaag ggctttggcc
    21241 atgttggcgg tggcttcgac ggccgcggcg agagccgggg tgttggtgag cgccccgcag
    21301 tagaggccgg caatgcttgt ggccgggaga ttgagaaaat ggccgatcac cccggcgacc
    21361 acggcgccgg ccgtcaggat gacgatggcg atggcgctga tgcgcagccc cctccggtgg
    21421 aacgaggtga aaaatcccgg gccggcctgc aggccgatcg catagacgaa cagaacgagc
    21481 ccgacgatgt aaatctgctc gggaaggatg aggcgtttgt cgagggcgga gaaggcaata
    21541 ccaacgaaca gcacggcggc cacccccagt ttgaaaccga ggatgcggat gttcccgatc
    21601 aggtagccca gcccgataat cacgaagagc aggaggaggt cgttctgggc aagcaggttc
    21661 agaatcgacg ccattacttc agcctttcac accgccgcac ggcctgtgtt tcacgagata
    21721 cccaccgccg tgccggggcg caactaaaaa gcggacaggc acctgacgcg ccgccggccg
    21781 gaccatacga cgtgcccgcc ggggcgcagc ccgcggccgc cggggccccc gcgagcacat
    21841 taatcccttg acgagtcggg gggattggtt atattcgaaa gtttaaccga cttgaggtaa
    21901 ggaagaaatg aaacggacat tccagccgtc gaaccggaag aaacttaacg accatggctt
    21961 ccgcaagcgc atggcctcca agggagggcg cctcgtcctc tcgcgccgtc gcgccaaggg
    22021 aagaaagcgt ctgactgtca aggttgcccg caagaagtaa ccagtcgtcc cgctgtctgc
    22081 ctcgctgcct gagtctcaag cagcgccggg cgatcgagga tgtggtgcag cgcgggcgca
    22141 cgctgcgcgg cgcggcggcg acattccgct ggcagccggc cgaccgtttc ggatatgtcg
    22201 tcgtcgtcgg acgccgccac ggccatgccg cccggcgtaa ccggatcaag cgcctcctgc
    22261 gcgaggcgct cagactgaat cgccaccgtc tcactcggga ggtccgaatc gcggtgctgc
    22321 cccggcttta tggagacgcc ctgcggtttg acattttgca tgcggaattc ggtcgtatat
    22381 tcgaggcgat caatcgccac gccgactaac ccccggccat ctgtggccgc ccgtcttctg
    22441 attttgctcg ttcgcatata ccaggcgacg ctctccgggc tttttgccgg aagttgccgc
    22501 ttctatccca gttgctcgca ctactcagtc gaggcgcttc gttcacacgg agctctcagc
    22561 ggattgtatc tctctgtcag gcgattactt agatgtcatc cgtggcaccc gggaggctac
    22621 gatccggtgc cgtcggcgaa agaaacccgt gaataagccg gccggctccg ggataaagac
    22681 tgaaaccaag ggtcaccgtg gacaaaaaaa ccatcatcat cgtcgtcatt cttggcgcga
    22741 ttatcatttt ctactatccg attcttcagt ttctcgggtt gtacgagccg ccgcgaccgt
    22801 cgtcccccgg gacagcaccg gattctaccg cggcggttga ctcttccgcc cctgttcccg
    22861 tgataaaacc ggaagcggtg caggaagtta ctgtgaaacc gcagccggtg gcggagatcc
    22921 cggcggacac cgtcggggcg gatacgatcg cggtagaaac ggagttctac cggattcttc
    22981 tatcgacgcg cgggggtggc ccggtgtcga ttcagttgaa ggagtacacg tacatcggcg
    23041 gcgcgaatgt ggaaatgctc ccggagtgcg aagccgccac cccgaacgcg gttttcgccg
    23101 gcgggacatt ctcgacagcg gcccttccgt ttcaggcgag tctcgcgccg ggccggtaca
    23161 gcgtcgcgcg cgagacgctg ccgatatcgt tcacttacac gaaccccggg ggcgggatta
    23221 tccggaagac ctatttcttc tatccggacc agtaccacta cgacctgacg ctcacggtgg
    23281 aggacacacg ggcgctcggt ttcgagcgga gctacacgct gtggtggaac acgccgctgg
    23341 ggatcaccga gagtcaggcc cgggcggact acagcgcgat gcaggccgtg accatgcagg
    23401 ggggatcgcg ggcgactctc gatgagtttc acgacaacca gctcgcgcag agtctcgacg
    23461 gttacaccga gtgggtggga gtgcgcaaca agttcttttc ggcgatcctc attccccgga
    23521 gccgggaggc gcaggcggcc gtcgccgagg gatccaagca gatgctggcg acgccggagg
    23581 ggcggattga gcagcgccgg atcaccgccg gcgcggtgat gacgttcgcg ggggtcgaga
    23641 cgatcagcga cagcttcacc gtctacgccg gcccgttgga ttacctgcgc atggatgact
    23701 acggcgtcga cctggaggac atgctcgaca tcggcacgat gccctttttc ggcgcgatcc
    23761 tcaagccgtt cgcgatcgcc gtgatgtggc tcttgcccaa gctgcacgac atcgtcccca
    23821 actacggcct cgtgatcatc ctcttcgccg tgctggtgaa gatcatcacg ctgccgctgt
    23881 cgctcaagca gttcaagtcg atgcaggcga tgcgggatct cgcccccaaa ctcgaggatc
    23941 tcaagaagcg gtacaaggat gacaaccagg ggctccagcg ggagacgatg aagctctaca
    24001 aggaacacgg ggtgaacccg atgtcagggt gcctcgtgat gctgccgcag atgccgctca
    24061 tgattgcgat gttccgggtc ttccaggcga caatcctcct ccgccatgaa ccgctgttct
    24121 ggtttgtcac cgatctgtcg cgaggcgcct cggggctgac cgacccctat atcatcctgg
    24181 ttgtcctcat ggtcgtcacg caaatgctgt catcgctgct gaccatgtcg ggaggcggac
    24241 agcagcagaa gatgctcacc tacatattcc ccatcatgct gggctttttc ctgtacagcc
    24301 tgccctcggg cctgatcatc tactggacgg ctttctccgt gctggcggtg gtcgattggc
    24361 tggtgttccg gcggaacagg gcgaaaaatc cgcagatcaa gacggcgtaa tatgggcgga
    24421 gcacctaaaa aaaaagtcct gatcacagcg ggatcgacac gatcgcggcg gtgatcacac
    24481 cgccgggaga ggggggaatc gcagccttgc ggctggccgg gccggccagc ctggccattt
    24541 tgtcccgaca tttccgaccg actcaggtga agtcgccggc ttcgccgtgg ctcttgcggc
    24601 acggcctttt caccgaccgt gaaggggcct caatcgatga agttaccgca gtgtatatgc
    24661 cggaggggca atcatatacg ggacttgaac aggtcgagat attctgccac ggcgggcacc
    24721 tggtggtgcg gcagatcctg cacgagcttc tgaccgcggg cgcccgagcc gccgagccgg
    24781 gagagttcac ccgcttggct ttcctctccg gacggatcga cctcgcccgg gccgaagcag
    24841 tcgcggaagt gatcgccgcc aataccgagg tgtcgctggc gactgcccga aatcacctgc
    24901 tgggcgccta caccgaacgg ctcgagcaaa tgcggcagga gattgtcgcg gtgatggccg
    24961 aggtggaagc gtcaatagat ttccccgaag aagagatcga gccggcgatg gccgccgggc
    25021 aacgggagag catcgatcag gttcggggaa agatcctcca gctggcggcg acttatcgcg
    25081 gcgggcggat tattcgggag gggtaccggg taacgatcgg ggggcggccg aatgccggga
    25141 aatcatcgct ttttaaccgg ctgcttcgac aggaacgcgc aatcgtcgct gccacgccgg
    25201 gaacgacgcg ggactatctc tccgaatgga tcgacctcca cgggtacgcc gtgaatctcg
    25261 ttgacacggc cggtattcgc agagggggcg gcgcggtgga gcaggccgga cagacttccg
    25321 cacgcaagat tatggaagag gccgacctgg tagtctggct cgtcgatctc tcggccaagg
    25381 actggcaaaa gcaactgggg gaggatctcg cgagcatcgg cgggctgccc gtggtgatcg
    25441 gggccaacaa gattgacctg gtcggggccg caccggcgat atcggaaact ccggctgaga
    25501 tcgagacgat ctacctgtcg tgcaagaccg gcgaagggat cgacaacttg gttggacatc
    25561 tggtggcgca catcaacgag gggatgcccg attttacctc cgggatgatc gtgacttcgg
    25621 cgcgacaccg gcaaaagctc gaaacggctg agacggcctt gcggtcggcc tcggaaaaga
    25681 ttagcttgaa agaatctccg gagctggtcg cgttcgattt gcgacaggcg gcgggggcgc
    25741 tggaagagat caccgggcgg atttataatg aggatattct cgatcagatt ttctcgaagt
    25801 tctgcattgg gaagtagtgt tgtttcacgt gaaacatccc gacatttgat caaagcgcaa
    25861 accgggacaa aaaagcgatc cgcccgacct aagcggagcg ttccctcaag atgatggtga
    25921 accgggattt cgacatcgta gtggtaggag gcgggcacgc cggggttgaa gcagctctgg
    25981 ctgcggcccg gatgggtaag agcgcggcct tcgtgaccat ggacccgcac cgactggccc
    26041 tgatgtcctg caacccggcg atcggtggga tcggcaagtc acacatcgtg aaagaggtcg
    26101 atgctctcgg cggcgttatg gggatcgcga ttgacgccac cggaatccag ttccgccggc
    26161 tcaacctctc gcggggaccg gcggtctggt cgacccgcgc gcaggcagat agaatcgggt
    26221 acaatcgatt catcgtcgag ctggtgcgaa attgcccgaa agtctcggta atcccgggtc
    26281 tggccggaga aattctcacc gagcgcgaga ccgtagtcgg aatccgcctg gaatcaggcg
    26341 acgagatctc atgccgggcc gcagtgatcg ccaccgggac tttcctgggt ggcctgatcc
    26401 acatcggccg gaaacagatt ccctccggcc gaatcggtga accggccgcc taccggctgt
    26461 cggagtcatt ccggtcgctc ggtttcgaga tcgggaggat gaaaaccggg acaccgcccc
    26521 gactcgacgg cgacactatc gactggggtg cctgccaggt tcagccgggg gatgagccgg
    26581 tgccgttttt ctccccccgc tcgcctcgcc gggtgttcca acaggtttgc tgccacctga
    26641 cgtacacgac ctcggcgact aaggcaataa tcggcgaaaa cttccacctg tctcccatgt
    26701 tttccggcca gatcaaatcg cgcggaccgc gctactgccc gtcgattgag gataaattct
    26761 tccgcttcgc cgacaaggag cgccatcagc ttttcctcga gccggagggg aacgggacca
    26821 atgaagtata tccgaacggc ttttccactg cgctccccga agaggtgcag gtgaaggcga
    26881 tccggacggt cgccgggctt gagaatgtcc gggtgacccg tccggggtac gcggtcgagt
    26941 atgactactg cccggctcac cagatcaagc cgtcgctgga gaccaggcgg atcaacgggc
    27001 tgttcctcgc cggacaaatc aacgggacat cgggatacga agaagctgcg ggacagggga
    27061 ttatggccgg catcaacgcc gttctccgga tcgaacagga agcgccgttt gtacttgacc
    27121 gctccgaggc gtacatcggg gtgatgatcg acgacctgac aacgcggtcg actaccgagc
    27181 cttaccggat gttcacctcg cgggctgagt accggctcac gctccgcgaa gacaacgccc
    27241 gcgaccggct ccagtcctac gcccggcggt tcggtctcat tccggaaacg gtactcgccg
    27301 gtttcgatga tctgcagcgc cgaaccgatg cggtggtggc gatgatcccg aagatccgcg
    27361 tgcgggtgga tgaactcggt gaagcaggag cccggttcac gcgagtggag acgattagcc
    27421 tcgacaacct cctccgtcag ccgacgctct ctattaatga ggcggtggcg tggctgcgga
    27481 agttccccgg ccggttcccg gatgaccagg aggcgctcga gagggcagcc atcgtggttc
    27541 gttacagcgg gtacgtggac aagcagcaac gggaaatcga caaattccgg cgcatggagc
    27601 gggagacaat tcccgatgat ttcgatttcg cgggcgtgag cgggcttaag aaggaagctg
    27661 gggagaaact ggcgcggttt cggccgacct cgctgggcca ggcggggcgg atcgagggga
    27721 tcactccggg ggatgtagcg gtgctttcgg tctaccttca gaggcacaag gcgctgtcgg
    27781 catgaacggg acaagcgagg ggatcaggct tgacgtggcg gcgtttctgc gcgaacggga
    27841 tcctaataac aggctcaccc cgtatttcga gctcctcgcg cgggagaaca gccgggtcaa
    27901 cattgtttca cgtgaaacat tgcccccgac cgccccgtcg gggcgcgatc ggttcgaggg
    27961 actgcggcgg ctcacggcgg aatcgatcta cccgatcacc gaactccacc gagcgcagac
    28021 gaggagttat ctcgacatcg gcagcggtgg aggattcccg gcgatcccca ttgtgctcac
    28081 cgcaacgatc agcgacaccc tcctggtgga acggacacgg aagaaagccg ccgcgctgcg
    28141 ccggatactg atggagctgc agcttcgggc ggagatttgc actgaatcgt tcgagtcgct
    28201 cgtccttaag gaaggatcat gcgatctgat cacgcttcgg ttggtgcggc tcaccgaccg
    28261 actggccgcg cgaatccttc cgctgttggc gccgggcgga gatttcgtct actatgcact
    28321 ggccgatatc tcggctgatt cctccagatt tctgaccaga caactgcgat tcaccgccgg
    28381 cggggggcgg gatttccaca cggccacgat agttacgaga aaacctgagt gactctttca
    28441 cattttagtt gattctgagg gtctctgcgc atagtattcg gttttcgagt gtgtggatat
    28501 cgtcaacaag gattgttgct tggccagaat tatcgcgatc gcgaatcaaa agggcggcgt
    28561 gggcaaaacg accacggcgg tgaatctcag ttcctgcctg gccgcggccg aattgaaaac
    28621 ctgcctgatc gacatggacc cgcaggcaaa ctccaccagc gggttcggat tcgacaaacg
    28681 aacaatcgag acatcaatct acgacgtcct gctcggctcg gcgacgctgc gcgaggtgct
    28741 gcgggagacg gagctctcgt acctccacct ggcgccttca tcgatcgccc tggtgggggc
    28801 ggaggtcgag ctggtgtcgg tgatgtcgcg ggagacgcgc ctgcgcgcgg cgctggcccc
    28861 ggtgctcgat gattacgcct acatcctcgt cgactgcccg ccgtcgctcg ggctgctcac
    28921 cgtgaatgcg ctgacggcgg cgcacagcgt gatcatcccc gtccagtgcg agtactacgc
    28981 cctcgaggga ctcggccagc tgatgaacac gatccgcctc gtgcaggagc atctgaaccc
    29041 gacgctgcag atcgaggggg cgctgatgac gatgtacgac gggcggctca acctgtcgcg
    29101 gcaggtgctc gacgaggtga agaagcattt cggaggaaag gtgtacgata ccgtgatcac
    29161 gcggaacgtg cggctgtcag aggcgccgtc gttcggcaag ccgatcattt tgtacgacat
    29221 cctgtcaacg ggcgccgaga actacatggc gctcacccgg gaggtactgg cacggtgaag
    29281 aagattgtgt tggggaaggg gctcgacgcg ctcatcccgg tcgagagcgg cgaggcgggc
    29341 ggggagcggc ggatgcgcca ggtggcggtt gacctgatcg cgcccaaccc gctccagcct
    29401 cggcgggagt tcgacgaggc ggggctgctc gagctggccg agtcgctcga gcgcaacggg
    29461 atgatgcagc cgctggtagt tcggcaagat gggtcgacct atacgataat cgccggcgag
    29521 cgccgctacc gggcggcccg cctggccggg ctgagccagg tcccagtgat agtgcgcgac
    29581 gagagcgacg acgcgcggat gctcgaactc gcgctcgccg aaaacctcca gcgcgaagat
    29641 ctcaacccga tggaagcggc cgaggcctac cggacgctca tggagcgggg aggatacacg
    29701 cagcaggcgc tggccgatgc gatcggcaag agccgggcgg cggtggccaa tgcgctgcgg
    29761 ctgctcacgc tgccggaacc ggtgcggcag atgatccgcg ccgggcggat caccgaggga
    29821 cacgcgcgcg cgatcctgca gattgaggac gagcagggac agatcgagat ggccgagcgg
    29881 atcgccgagc agtcgctcac ggtgcgggcg gtcgaggagg aaacacgccg gcccgcgcgg
    29941 gcgaaaggcc gccccgccgg acggcgcctg cccccggcca tggccgaggc cgagactttc
    30001 cttaagcaga cgctgggaac cgcggtgcat atccaccacg gcccgaagcg cggccgcatc
    30061 gagatcgaat actacggcgt ggacgacctc acccggattc tcgaactgct cgccaaactg
    30121 tcgtgatcat ggcgcagtcg cggtatatca cggtgctgtt tgttcccgac ggaaaccggc
    30181 cgcagcgcgg gttccgggtg cgcacctggg tggtgcaggc gggcggggtg gcgctcacga
    30241 tggcgctgct ggcgataata attttcttcg ccacctattc gaagataatg gttcgggcga
    30301 ccatgaccga tagactgaaa gaggaaaacg aacaactgct gaaataccag tacaaggtgc
    30361 gcatactcga ggataacatc gcggagatgc ggcaggtggt cgcacggctg acctcgctgg
    30421 ccgggatcga ctaccagttc ccggagattc cgagcgactc ggagctgttt gcggcgatcg
    30481 atgaggcgcc ggcggcggcc atcaaccgtc ccgccaccga tttcacgatg ccggccggac
    30541 tgccgatccg cggattcatc acgcagaatt tcgaggtcaa gaacgatgcc cgctaccact
    30601 cgggagtcga cattgcctgt ccggtcggca cgccggtgct ggcgacggcg gccgggctgg
    30661 tgattttcgc cgatgtcgat tccacgtacg gcaacatgat cgtgctgcgc cacaacgaca
    30721 cggtggtgag cgtgtacggc cacctggaca ggattctcgc gcgggccggc gaggaggtcc
    30781 cggccgggag ccggatcggg ctctcgggga acacgggcat ttcgaccgcc ccgcatttgc
    30841 attatgaagt ccgtataaac gatgaaccca taaatcctct ggaaaatccc tatgatcaag
    30901 agaaacgaca gtgaagcgga aggactcatg aacaccttaa tcggcaaaga cacagtgatc
    30961 accggaacgg tggatgtcaa gggcgccctc cgggtcgacg gcacggtgaa gggcaaagtg
    31021 atttgctcgg actgtgtaac ggtcggtact acgggcttgg tcgaggcgga aatcgagtcc
    31081 aaggaagcga tcgtcgccgg ccacatgcac ggcaacatca acgcaaccga aaaaatcgaa
    31141 ctccaggcca actgcgaaat ggacggagac ctgcgcacca agtcgctcgt catcgagcag
    31201 ggcgccgtct tctgcggcaa ctgcaacatg aaggggacta ccgcctcgcg caaagagttc
    31261 gggttccttc cccccgagca gaatcgggaa acggtcgcga acgtgatcgg cgacaaaaag
    31321 gaagtcaaga agtaacaatt cgccgccagc tgtggagaaa tacccgaatt tgtggataaa
    31381 cgatcaagtt tttgtcggcg aaggacatag tgtcgagaaa ttcaccggga aagacacccg
    31441 ttatccacaa cggtattgcc tcttaatgtc ctgaaagaaa aagacttata ccttgttgcg
    31501 tcaagtggcc gattacgacc ggataggcta gcggctgata caaaaagcct tgcgctatcc
    31561 ggtcttttca tgtttctcct ggtgatagtt cggagtcgcc ggccggagtt tcggtcgcaa
    31621 gcgaaaccga aagaaaagca cggactataa gagaaatatg gtgatccgca cccgacccgc
    31681 gggtgcgcgg cgtaccggct acctggatga tccggaggat gaatggcaat ctcgggaacg
    31741 caggctgacc tgacagcggt tgagaacctc aacagcgcct atcggcgcat caaggcggag
    31801 atcggcaaag tcattatcgg ccaggagaga gtgatcgacc aactcctgat ctccctgctc
    31861 gcctccggtc actgcctgct cgtgggcgtg ccggggttgg ccaagacgct gttgatttcg
    31921 accctggcgc gggtgttgga tctcagattc aatcgcattc agttcacgcc cgacctgatg
    31981 ccctcggata tcaccggaac agagatcatc gaggaggacc aggcgaccgg gcgacggcgg
    32041 ttccggttcg tcaagggacc ggtgttcgcg cacatcatcc tggcggatga gatcaaccgg
    32101 acgccgccaa agacgcaggc ggcgctgctg caggcgatgc aggagcatga ggtgacggcg
    32161 gcgggcgaga cgtataaact cgacgaaccg ttcttcgtgc tggccaccca gaaccctatc
    32221 gagcaggagg gaacctaccc cctgccggag gcgcagctcg accggttcat gttcaacgtg
    32281 ttcgtcgatt acccctctga gcgggaggag cgggagattg tgcgatcgac gacggcggtg
    32341 ctggactacc agttgaacaa gatcctgtcg ccgcaggaga ttatcggctt ccagcagctc
    32401 gtgcggcggg tgccggtctc ggaccacctg gtcgagtacg cggtgaagct ggcgcgcaag
    32461 acgcgcccgc acgagccgga cgcccccgac ttcgtgcgga actgggtgaa ctggggggcg
    32521 ggaccgcggg cgtcgcagta tctcatcctg gccgccaaga cgcgcgcgat cctcgacgga
    32581 cgtccgacgc cggggccgga ggatgtgcgc tttgccgcct acccggtgct gcggcaccgg
    32641 atcgtgacct cgttcaacgc cgaggccgac ggggtggata cgatggagat tatcaagcgc
    32701 ctcctggaga cggtgaaaga gcagtagcaa cgagtcgcgg tgaaagcgag ggccccggct
    32761 gcgtgtggca gccggggtct tttttttggc cggtcggcga acttgttttc gcctctggac
    32821 aaactcacgc aaacatctac actcaagcca tcagattacg cagagtccag cgcaggtaac
    32881 cttgttgtcg gcgagaaagc atgaccacga gtgacgacag ccgtgaccgg acgcagaccc
    32941 acatccagtt gacggttgat ctgatggtcg ggcgctaccg cttgaccggc ttctcgggag
    33001 cgggcgggat gcgttaagtc caccaagcgg aggacaccac gcccggacgg gcggtggccc
    33061 tgaagtttct cccgcttcac ttggcggcgg atgccggggg cagaaagcgc tctcaggggg
    33121 aggcgcaggc agtggcgaga ctcagtcatc cgaacattgt caccatccac gaggtcgggg
    33181 ggtttcaagg ccggccgtcg ggcgtccgca agcaacgagg tcaggcgggt tcgcgtcggc
    33241 agcgacggtc tcgacacggt cggcacactg cctgaagggg cgtcaaacgt caaactcctc
    33301 ccaggcgaga agtccgtcgt ttactcggtc atgcactccc gctcggacat ccggatagtc
    33361 gagaattttg gccccgaggt gcggcgctag gccgccctct ccccttcccg aaaagggttt
    33421 gcatccctgt accgattgta tcatcgtatg cccgtgatcg agaaaattcg gatatccgac
    33481 ctgccgccct acggaaagac cttcgtcggc atcttcgccg ggctggcggt ctgcatgctc
    33541 ttgtgggtgg cgctcatcta cacggtcgat cggggaatcg tgcactccga ccggctgccg
    33601 gcctacctga acgccgaccg ggccgggcag ggggcgccca ccacggaaga ttcggcacgg
    33661 aacgagcggc tcgaacagga ggagatcgcc gaagaggccg aggaggtggc ggtggattcc
    33721 gaagcggtgc tggcgccgat ctgggacagc gatctggcgg gacgggaagt acacgccgac
    33781 agcgcttccg ccgcggcgcg gttcgggaaa gtcgattcga cgatcgaggc cgagatcgcc
    33841 agacacgggg tggggccggg taccggctat gactacaacc cggaccggga ggatttcgat
    33901 gtcgatgagt tcgagcagcc caacctgcgg cgcaatgccg ggctggcgca cacgcacatc
    33961 aacggccaga cgctgattta cttcacgctc gggctggtct tcctgttcac ctcggcggcg
    34021 cccaagatca agacgacggt gtactggctg ctcggaagcg cggttgcggc acacgcggtg
    34081 gggctgtcgg gagcggggtt ccactggttt ttcgatgacc tcctggcgat cagcggcctg
    34141 gtgatcgtgg ggacagccgg ctatatgctg ttctggatat ttgcggatct gggaaagaag
    34201 cgggcaacgc cggcggggga gtaaaagaga gagacgcgca tgccacaggc gatgaatgcc
    34261 caccccccgt tcgcaatcgc aatccacggc ggggcgggga caatactgaa agagaaactg
    34321 acgcccgcgc tggaggcgga gtacctcgac gggttgcggc ggtcgctggc ggccggctac
    34381 caggtgcttg accggggcgg gcgggccctc gatgcagtcc aggcggcggt caatgtgatg
    34441 gaggactccc acctgttcaa tgcgggcaag ggggcggtgt tcacccacga ggggttccac
    34501 gagcaggacg cgtgcatcat ggacggagcg gggcgggcgg tcggggcggc ggccatgctc
    34561 cggcgggtcc gcaatcccat caacctcgcc cgggcgatcc tcgaatactc gccgcacgtg
    34621 ctgctcgcgg gcgagggggc ggaaaagttc gcgcaggaac acggcgtgcg actggtcgaa
    34681 ccggagtatt tctacaccga gcagcgctgg cagcaacttc agcaggcgct ggcgaaagag
    34741 cggcaggagg ggggcgacca gacgcgactc gatcattccg acgaccggga caaaaccggg
    34801 acggtcgggg cggtggcgct ggaccgggcc ggaaatctcg ccgcggccac ctcgaccggc
    34861 gggatgacca acaagcggtt cgggcggatc ggggacagcc ccattatcgg agcggggatc
    34921 tgggcggata acgccacctg cgccgtgagt gcaaccggcg tgggggaata catcatgcgg
    34981 gcggtcgcgg cctacgacat cgcggccctc atggcttacc ggggactcag cctggccgag
    35041 gcggccgacg aggtggtgac gggaaagctc accgcgctgg gaggccaggg cggggtggtc
    35101 gcggtcgatc gggccgggac gatcgccatg ccgttcaaca gccccggcat gtaccgcggg
    35161 tatcggtatc cgggccgcga tccggtcgtc aaaatcttcc gcgacgaata gccctctccc
    35221 caagagcaga ccgagcgggg acgctttcga cgcccgcccc gcaaatcgct gtgcaccagg
    35281 cttacggcgc ggcgccgccg gcgcggagct ccgccagggt ctggcccagg agacgattga
    35341 attcctcggg gcgctcaagg aagaggaagt gcccgacgcc gctcatgagt ctgacgtcga
    35401 aagtcggctc gatttcgcgg ttggccgcga ggttggtcgg gtacgagtcg ctgctgaccg
    35461 cgcgaatcgg tttgccggct tcccgcagca ccgggagggc gtcgtagacc aggaggttcc
    35521 cgagagtggc caggccgacc gcgggatcat cggcctggaa agagcggatg atgcggtagg
    35581 caatggcggt gtcgacattc tccggcagca tgacgctggc gaaccaggcg acggtggagt
    35641 cgtagttccg gcggaacggc gcgaggaact gctcggcctg gtccggggtc cagcgttcgc
    35701 gaaaatcctg gtaggtgtcg gcgccgatca gggcgatgac ccggtccggg gcaagacggg
    35761 cggcctcggc gatgatgacg ccgcccatgc tgtggccgac caggaatgcg tgcgcgatcc
    35821 cggccgcgtc aaggaccgcg acgacatcgg cgccatacgc atgcggagtc cacgcggccc
    35881 ggttcttccc cgaacggccg tggccggcca gatcgagggt gacgaccgtg taggagtcgc
    35941 tgaaagccgg aatctgtttc tcccagaagc tgccgtcaat gccccagcca tggacaaaga
    36001 ccagcgcggt gtcgccgctg ccgtggacct cgaagtagat cgggacactg tcggtggaca
    36061 gggcggaatc gacgcggagc gcggcggagg catccgccgg ccccccttct cccccgcccc
    36121 ggcaggcgaa cgcgagcacc acgagcagca ctgcagcagc cccggtgcga ggccaaatcg
    36181 aatgcagcat atgtttcctc tcttccggtg gccggcgcgg cgcgggcggc cggacgcacc
    36241 gccggcgcgg cgcacgatca cggcgggccg ccggaccgcg ttcgcggaaa tctacggagt
    36301 ccggaaaggg ggtcaagggg tattttgtcg ttggcggccg atcacatgct gggttccatc
    36361 tgcaccgcgc ggtcccattc gacggcgaaa tactcccgga tgaaccggcg gatgacctct
    36421 tcgagccgcc gggtggccgc ccggtgtccc tcggcgtaga gatcctcggc acgggcggcg
    36481 aagacgcgga cgacgtccgg gtcgaactgg ctgccggcgc cggcgacgac catatcgcgg
    36541 gcctgatcga aattgtacga cagcttgtag ggccttcggc tcgtgagcgc atcgaagacg
    36601 tcggccacgg cgaatatgcg ggcggtgagg ggaatctcga ccccgcagag cccggccagg
    36661 tagccggtgc cgtcccaacg ctcgtggtgg tagcggatga cgtcaatcgc atcggcaagc
    36721 caggtgacca cttcgatgat gccggcgccc cgggacacgt gggacttgat aatctcgcgt
    36781 tccgggggcg tgagttcgcc gggcttgatg aggacggcgt cgggaatgcc gatcttgccg
    36841 atgtcgtgaa ggaaagcccc cttgatgagg gagcggatcg tctggcgggg caaacccatc
    36901 gcctcggcga tccggacggc gaagatggtg acgcggtagc tgtggtcgtt ggtgccggtg
    36961 tcgcgctcgg cgatggcgcc gccgagcgcc cggatcatgt ggaggtggct gcgcagaagg
    37021 tcgcgttcga gggtcagcag gagtccctgg agaatgcggt cgtagtcaag gagctggccg
    37081 cggtcgagcg gaccgaccag ccgatccggc tcgcacgagg cgagcagccg ggcgcgcagg
    37141 agctcaagca gggagatcga gacgaggtgg aagtactcat cgggagaaag tcccggaggc
    37201 ttctcgctca ggagagagtg gcagatcctc tcggccatag ttccgattat tcccgattcc
    37261 tcgagccggg ttttcaccga ctccttcagt tcttcggatt caatcataca accctccacg
    37321 agaaccaggg gtcaggaccg acagagccga ggtgagagcc gacgatgcga cctgtacaat
    37381 ctatataata gcgccggggg acgaaaagtt cgggcggtcg gggccccggc ggcccccgac
    37441 acggtcgtga tgagctactc cgacgggcgg aggtcgagcc ggacaccttt gggaagagtg
    37501 ggtgcgaagc gggtcaggag gatgcgagcg gagtcggcgg cggcgcgatc gagcgcatag
    37561 aaagtcactt ttctgtcggc cgcgctcgtg cggcggtgac cgaggaagag atcggccacc
    37621 ggaatccagg aatccggaat gtgcgcgaac caggattcgt agatgatcgc cagacgggcg
    37681 cctcccgcag ccgccgcttc ggtcatccac tgaccgctgg aggcctcctt ccggcgcccc
    37741 acgttgcggc tgtccgccag gccgaccagg tcgaccacag tctcgtcatt ctggaaagcc
    37801 acccagccga tatcgttggc ggccacgggg ccatggtagt attcggtgat gaagcggtgc
    37861 atctggtact gctgctggta aatgttgctc gcggcgattt gcaggtcgct gaggcggtga
    37921 agttgcggaa aagcgacgag ggcgacggct gcgacggcga ggaccgccgc tcgcaccccc
    37981 ccggcccgct gcaggaaccg tcccagggga gccgaggcca ggaacacggt cagtaggagc
    38041 gtactcagca ggatgtacat gtcgtagcgg gcgtgaaacc agccccccag ccgtccgacc
    38101 gacaggtgca agacaatgcc ggcggccccg gcggcggcga gcaggcggtg ttctcggcgg
    38161 cgggagcggt ccagcgcgac ggcggccaag gggatgaaga cgacagccag gagggtcccc
    38221 tccccactca gcaggttgcc gcggaaatgg tccgtgatgg cggctatcga ccagccggag
    38281 caaacgacgt cggagcgcat cgccaccgag gtcggcagcg ccccgagccc gagggcggcc
    38341 aggtacccgg agtacccggc ggccagcccg agggcggtca aagcggagac gagcgccacc
    38401 cggcgatgcc cgctgaggta gaggcacacg gcggccggca gagtcaacgc gaacatttcg
    38461 taccggacaa gcggggcggc gatcagggcg gccggcagcc accggggaac gcgctgacac
    38521 gacagagcgc acaccagacc atgcacgacc gcggcggcga gcagcaactg cagggagtgc
    38581 tccatgccgg agaagatgag gccgatgagg ttggtcaccg gaacgagcgc cgcggcgacc
    38641 caggcggcat atttgaccgg gttcgcaaga ccggcggcgc tgaaaccatg cgccatcgtc
    38701 cggaccatga gcaggacggt ggcggcggcg cacgcgagat tgacgacgaa ggggacgacg
    38761 gccccgaagg aaaggagcat gaagggggcc agcaggaagg gccagaggat gctcgacgac
    38821 ggcgcgcaga cttcgccggg attaatgccg aaatggccgt gggcgatgcc ctcggcgagc
    38881 gccatgtgga tgtacgcatc atcaagcgtg tacgtcagat ggccgtcgtt aatgcccaga
    38941 ttgagagcaa ggtggacagc ggcgatgacc aggaggaggg ccagcggcag acggacccag
    39001 gcccggtcgc ggccggggcc ggcgggcgcc agcgggggcg aatcggtcac ggcggtcgca
    39061 ggggatttct gcatcacctg gctcctcaca gtgcagtcat tggggtagtg gcctgcgcgg
    39121 caagatggca tgcggagggc ggcggtgtca atggttttcc ccgcacggcg gtttcgccca
    39181 gggcggattc ccgggggggc gagccatgcg gacagtgcga caacccgttg gcaaacaaac
    39241 aataagcgcg gaccggcggc ggggaggcga aaatggacag gagaccgcta aatttggtgg
    39301 actttcggcc gatcctagcg tatagtagtt aacaatagcg gttgccgtat tcgcttttcg
    39361 aagtgttctc aataccgttg aatctccgaa aaagctcgac cccgggaccg atgcgcaata
    39421 gtgcgcttga atgtgttttc tccggtgatg gccgcaacgg ccgccatcgg ggcgaacgcg
    39481 agtggagagt ggaatgaata tctacgtcgg caatctgtcg ttcgacacga cagaggagca
    39541 gttgcgccag gctttctcta gccatggcga ggtcacgtcc gtgaacatca ttagcgacaa
    39601 gtacagcggg cagtcccgcg gattcgcttt tgtggagatg cccacgaaga gcgaggcgat
    39661 cgcggcgatc agcgctctga acggacagga gatgaacggc cgggcgatca gcgtcaacga
    39721 ggcgcgcccg cgcgaagagc gcgggggcgg cggcggcgga cgcggcggat tcggcggcgg
    39781 cggacgcgga ggccacggcg gcggacgcgg aggccggagc ggcggacggg ggggcgatcg
    39841 gggcggttgg taaagccacc ggacaatgcg aagcaagaag gggccgagat tcggtccctt
    39901 tttgcataga ctggggccga cggggagcct cagaaccccg cgggcgagga gggtgaccat
    39961 ggccggacaa cgaaagtctt tcccctgcgg acatgccggt aaaggaaaat actgccatcg
    40021 ctgtgcggcg gccgaggccg agcgacagaa agtcgcgcaa atcaagggcg actggcagtc
    40081 gcggctgcgc gcctccccga ttccgctgga tcatctgccg aaaggtgtcg cggagaacgt
    40141 cctgcgggta atagaggacc tgaaaagcgg aaaaccgtat gaggacttga agggcaagcg
    40201 gttgatcacc atgggccagc gcgacgtgat ctcgataccg atacaacgac gataccggct
    40261 catttgccgc gaccgggagg gcaaaatcga gcctatcgaa gcgataacgc acgaggtgta
    40321 caacagccgg ctgtcctcgg gcggctggcc gaagtgaggc cgacggcgaa gtcgggatgt
    40381 tgcgcggagt acggcccggg gcgggagggc tactacgctg ctcccgcgcg gaggatgccg
    40441 gatgcggcgc agcgccagtg agaaggcaag agcccggagg ctcttgccat ctcgctaccg
    40501 cccgggtcgg gatccccagg gactccctcg ctgcgggcta gtactcgccc accgtgaaga
    40561 atgtggaatc ggcgtatttc agatgcggcc cggcgacgct ggcggcgctc gtgtcgacat
    40621 ggaagacgta ggcctgtccc accacgggac ttgccgtcgg attgaaactc acccgcttat
    40681 caccatccga ccactggaac accccgttga ccggggtccc ggtcattgtg gtgaagcgga
    40741 tggcgctttc cgtactcgtc cgctccattg gcgcgttgaa ccggaaatac ggatcatcgt
    40801 accagtagaa agtccgcgtt cccggtgcag gatagacgcc ggctaccgct accggagcta
    40861 caccaaacgc gaacaccttg tcctcgccca aggcggcact gccgattaac ggcgcatccc
    40921 cgtcgatggt aatggtaaag gattgctccg gaaagagctg taccggctgt gtcgggaaga
    40981 agtcataata tccgacgtca tagtagcttt cagcgaaata ccagaagccg ggaagggggg
    41041 gatctatcgt gacggcgtcg ttcagggcat tttcgtccac ttcggcattg aaccgaatcc
    41101 gtgcgtagaa agctccaaac gggctgagcg tcgtttgtgt ctcgtaaaac cccgatccgt
    41161 tactgacggc aatttgcgtc acagtcagcg gttggatctt gagaacgacc atttcatcgg
    41221 cttccgtcgt cccgccgtga gaagatgcca gggtagaatc gatggtcacg gtatatgtct
    41281 cgcccggcgt cggttgagag agtatgctca cccgcagttc cctagtgtca ctgtagtaat
    41341 cggagcttac ctccacctga agctccagag gagggtcaac ggagacggcg tcctcggcgg
    41401 tctcaggatc caggtcggaa ttgtaatcga actcgaacag cgtctgcggc ctgggcggga
    41461 gcgcattggg agagtagttg tatgacctat acactacccg aagcggttcc gtatggaacc
    41521 cgatggtgtc cggggtggca cgtccggcac ccgcaaggca atggagtgcg gtatcgacaa
    41581 tgatcgagta gtcggcgccg cacgcgaagt cgccttcgac ctcgatgcgc accgtgtaga
    41641 ggttcgactg ggagaggctg gccgtgaagg ggacatcggg ttcgattctt acggcggtca
    41701 ggagagtgga ctcatctaca taggcgttga accgaagatc acagagccat ccggagtgac
    41761 cgggtgagag atccgcgaga tcataggagg agttgatttc ggtgagagcc atcggggcgg
    41821 tgcggaagga gaaactcagc gtatcacccc acggcccgcc gtccgccagc gccaactgct
    41881 ccgttatgtg tacatgatag ttctcgtccg ggagcaggcc ggacgtaggc tcgagagcgt
    41941 aaatgtacga tctgcccgaa tagtcatagc ctcgcgacca cgagtcgctg acgggtggcg
    42001 tgatgaccac ggcatcatcg agtgaggaca ggtcaagttc gacttccgat ctcagctcga
    42061 gagaagtagc cgccgggaca accgtgtcgc catcggcggg atagaacgaa acgatcggcc
    42121 acgaactggc gagaatgatg cttccgaggt tgatgaaccc ggccccttcg atcgcgattc
    42181 cgcccagcac cttttcccga tcatccgagg cggcaaagcg gacttcgtac aaacccgggg
    42241 gcaggttctc aagggtaaag taaccttcgg catcggtcgc cgtcgacgcg atttgctcgg
    42301 attgccaggc gctgacaagg acgccggctt ccggcgggga gacgcggccg aaaactccgc
    42361 cggcggcagt gtcggtgttg tagatgttat tagtcgtttc gtcgccgcag ccgccgacaa
    42421 gcagcaggag gatggccaca aacgcgatgg tacgcataca tgactccttg cggaaaagct
    42481 cggatttcga aaccctgcag tccggccaac cggggcggca gcgacgccac ccccggaggc
    42541 cagcaccttc gggatgtagt caacggcccg ggaggagccg gcgcaagcac tttcgaccgg
    42601 aacgcggagt tgccgaaaaa cgcgcttgcc gcaggggact gctttccgta tactatcaag
    42661 ctatcggaaa accgcttagc cggcgcgtgt gtaactgatt atgaaattcg acagtctcaa
    42721 agataacttc acgatcccgg ccgccgagga gaagatcctc cgcttctggg acgagaacga
    42781 cgttttccac aaggcccaga aagcccacca cgaccggccc cacttcgtgt tctacgaggg
    42841 gccgccgaca gccaacggac ggccggggat ccaccacgtt atcgcccgca ccgtcaaaga
    42901 cctggtctgc cgctacaaac acatgcaggg gttccgggtc gaccgcaagg cgggctggga
    42961 cacccacggg ctcccggtcg aaatcgaagt cgaaaaacag ctccggctgg agaccaaatc
    43021 ccaggtgctc gaatacggca tcgggaattt caacgccaaa tgccgggagt cggtgttcac
    43081 ctttctcgac cagtggaacg aaatcacacg gcggatcggg tactggatcg atctcgacga
    43141 cgcctatgtc acgctcacca acgagtatat tgagacggtc tggtggatcc tcaagacgtt
    43201 ctacgaccgc gacctgctgt acaaggggta caagacggtg ccgttctgtc cccggtgcgg
    43261 gacggggctg tcctcgcacg aggtggccca ggggtaccaa ctggtcaagg atccctcgat
    43321 ttatgtcaaa gtcccggcgg ccgacgagga tttcaagtac ctggtgtgga cgacgacccc
    43381 ctggacgctg ccgtcgaacg ccgcgctctg cctgcacccg gaggcgacct atgtgctggt
    43441 ggagcacgag ggggagaagc tggtgctggc cgaggccctg gtgccgcggg tgttcgggga
    43501 ggacaagccg gcgctcaaga aatggcgggg gagcgaattc ctcggacgcc gctaccggcc
    43561 gctctttgat ttctataccg accaggcgga cacggctttt gtcgtgatca cgggcgggtt
    43621 tgtcacgctc gaggacggca ccgggatcgt ccacatcgcc ccgggattcg ggcaggacga
    43681 ctacgaagtc ggcctccagc acggcctccc cgtgttccag gcgatcgagc ccaacgggat
    43741 attcaaagcg gtggcgggga aatacgccgg gaagttcatc aaggacgccg atcccgagat
    43801 catccgcgac ctcaaggcaa ccggggccct ctataagaaa gagcagtacg agcacaacta
    43861 ccctttctgt tggcggtgcg acagcgcgct gatctacatc gcgcgccagt cgtggtatat
    43921 ccggacgacg cagttcaagg agcagttgat ccgcaacagc aacgcgatca actggcagcc
    43981 ggacgaaatc cgcaccggac ggatgctcaa ctggctggag aacaacgtcg actgggcgct
    44041 ctcgcgcgag cgcttctggg ggacgccgct gccgatctgg gtgtgcgacg acgagaagtg
    44101 cgggcgtatc acggcggtcg ggtcgatcgc ccagctccgg caggagggga tcgacgtacc
    44161 ggccgaggtc gacctgcaca agccgatgat ggacgaggtg aagctccggt gtgcctgcgg
    44221 ccaaaccatg acccgggtgc cggaggtgat cgacgtctgg ttcgactcgg gggcgatgcc
    44281 ctacgcccag tggcactacc ccttcgagaa ccgggaggag ttcgcgatca agtttcccgc
    44341 ggagttcatc tccgaggcgg tcgaccagac gcgcgggtgg ttctactcgc ttttggcgat
    44401 cgggaccatg ctcttcgacc agtcggcctt caaaaacgtc ctcgtgctcg agatggtgct
    44461 ggacaaggac gggaagaaga tgtccaagca caaggggaac gtggtcgatc cgtttgtcac
    44521 ggtggccgag tacggagccg acccggtgcg ctggtacatg gtgtcgacct ccaacccctg
    44581 gctggcgacc aagttcgacc tcggggcgct gcaggaggtg gttcggaagt atttcgacac
    44641 cctgcgcaac acctactcgt tcttcgccct gtacgccaac atcgacgacg tcctggcgcg
    44701 ggcggccgcg gccgggcaga cggtcgaggc ctttctggcg gataaggccg gggagccgac
    44761 cccgttcgac cgctggatca cctcgcgcta ccacagtctc gcgggcgagg tggccgccgg
    44821 cttcgatcgg tacgacatca cgccggcggt gcgcaagatc cagaatttcg tgatcgagga
    44881 gctctccaac tggtacgtgc ggaacaaccg gcggcggttc tgggcgaagg gggacgagcc
    44941 gtcgaaaatg cgcgcctacc tgaccctcta ccgggtgctc gagggggtgt gccggctgac
    45001 ggcgccggtt gccccgttcc tctccgagct gatctggacc gagctcatgg ggccccagcg
    45061 ggagaaattc gggatcccgc tgtcggtgca catgaccgag tttcccaagg ccgacgcggc
    45121 ggcggtcgat gccgagctcg aggccgtgat ggggctggtg gagcggatcg tgtcgctcgg
    45181 acgggcggcg cgggcgcgca agaatctcaa agtgcgccaa ccgctggcgc acatgatggt
    45241 ccaggcgccg ccccagattc cctgggaacg ggtggccgag tatgcgccga tcatgaggga
    45301 cgaactcaat gtcaaggatg tgacgccggc ggagaatatc gaccggttcg tctcgtacgc
    45361 ggccaagctg aatttcaagg cggccggtcc gaagctcggc ccggcggtga aagcggcggc
    45421 ggccgcggtc gcggcgctgg cctccgctga cgtccggcgg ctggccgaga ccggcaggat
    45481 cgagctggcg atcgaggggc ggcgggtcga actgggcgcg gaggaggtcg aggtgatccg
    45541 gagcgaggag gccggatggg cggtcgaaag cgaggggggg acgacggtgg cgctggccac
    45601 cgagctgacg gaggatctga tcgccgaggg gttcgcgcgc gaactcgtca acaagatcca
    45661 gaacatgcgc aagtcctcgg ggtttgaagt aaccgaccgg atcgccgtcc gggtggcgtc
    45721 gacgacgcgc ctggcggaag cggtccggcg gttcggcgag ttcatccggc gcgagacgct
    45781 cgccgaggaa ctcgagttca cggtcgacgg gcacccggcg gagggtaccg agtggaacat
    45841 caacggcgaa ccgagccgga tcgctgtgcg gaaactgaaa accgggacga cggcatgaag
    45901 aaaaccgaga aggagaagct gcggaagctg ctgctggcga agcggaagaa gctggtcgag
    45961 gagatgaagg acacgctgga gagcaatttc tcgacgacga tgacggattc ggacggcgac
    46021 atttcctcct actcgttcca catggcggac caggcgaccg acgccatgga gcgcgagatg
    46081 gcgttcatgc aggcgtcgaa gtcggggcgc ttcctctacc atctcgacga ggcgctgcgg
    46141 cggctggaca gcaaagattt cggcaagtgc atcaagtgcg ggaaggatat cgggatggcg
    46201 cgcctggagg ccgtccccca cgcccgtctc tgcattgcct gcaaagaagc cgaggaacat
    46261 ggccgccgga agtagccgcc ggctgaccgc ccccgccctg gtgatcctcc tggtcgcggc
    46321 cgccgatcag gcgacgaaat tctgggcggt ccagacgctg ccgctgcggg agccggtcgc
    46381 cgtgctcggc agtttcgttc agttcaccct cgtgtacaac gaaggggggg cgctggggac
    46441 gcggctggcg tcctccagct actacctcat cacctcgctg atcattcttg ggatcgtgct
    46501 gtactacctc ttcgccaacc gcggcgccgc gcgggtgtgc tatccgctgg cgtttatcgc
    46561 cggcggggcg cttggcaaca tcatcgacag gatccgcatc ggccaggtca tcgacttcat
    46621 cgatatcgat ttcttcgaca tccacctcgc cggctacgag ctggagcggt ggtggacatt
    46681 caacattgcc gatgctgcga tcagcgtctc catcgtgtac ctgttggcaa gtgtctttct
    46741 ccaccaaccg gcgcgcgatc ctcgccggct gacggaaagc ggcagcgaat cggcttgagg
    46801 agcgcgccgt ggcggggacg gatctgtgcc acggaattgt cgtgataacc gtatgtgaag
    46861 tgtgcggttt gtcgggggcc ggttatagaa aatctcattc ggccgttgcg cggcgcgggg
    46921 cggccgattt ttcatgagcg gtaagagaca ctaatgtaat tacttccgga agcgcggaaa
    46981 ttaatccaca tcgggcttga cgagccgcag gagctagtct atcaatgctc atgaatagac
    47041 gcagacaccc gattgttgac agtgtgtgga cggtttttcc cgggggtgag ggaagctgtt
    47101 ctctgataga tcgttacaag cccccccgac aacgcatcat gtgaataact ctgtggatag
    47161 atatgcacga tcgctaagtt tccagcgcta tcccacctaa cgacaacagg gtgttctgtg
    47221 gacaactata catcatacaa acgaggggac gagtgtcttg actctgccac aagtcattcc
    47281 gaattcaccg cagtggaagg attgtctgcg gtatatcgct cagcgcgtca atgaacagtc
    47341 ctttaacacg tggttcaagc cgacgcgggg cggcatcaac ggcgacggcc agttctacct
    47401 ggcggtgccg aaccagttcg tggccgactg gatcaagggc cacttcgggg aggttatcga
    47461 tgaagcgttc cgggaggtgc tggggtcgag ttatcagttg gagtttgtga tcgcgcagat
    47521 accggaggtg ccggagcaga acgcgctcaa cctggaactg cccctgcccg agcggccgga
    47581 gccgctgccg gttccgcaaa cgcagcccct tgggcggcgg cacgatcaca atctgaaccg
    47641 gaagtacacg tttgactcgc tcgtggtcgg tgatttcaac cagttcgcgt tcgcagccgc
    47701 ccaggcggtc gccgaggcgc ccggtctgaa caagtacaac ccgctgttca tttacggcgg
    47761 caccggcttg gggaaaaccc acattgtcca ggccatcggg aatgcgatcc tcgaagcttt
    47821 ccccaacaag cagatcttct acgccaccag cgagaaattc acttccgatt tcatcgcctc
    47881 catctccgac cgcacgatcg ccgatttcac taaggtctac cgcgacgtgg acgtcctcat
    47941 catcgacgac atccagttct tctccggcaa agagtccacg caggagcagt tcttccacac
    48001 tttcaacagc ctccaccacc tggggaagat gattgtgctc actgccgacc gtccgccgcg
    48061 cgagatcaag ggtctggagg agcgcctgct gtcgcgcttc cagtggggcc tggtggccga
    48121 cctccaggcg cccgacctgg aaaaccgcac cgccatcctc tacaagaagc tggagacgga
    48181 agggatcacg ctccccgaca acatcgtgcg ctatatcgcc gaccaggtgc agaccaacat
    48241 ccgcgagctc gagggggcgc tcatccgcct gctcgcgtat gcttccctgc ggcaggagga
    48301 ggtcgacctg aaactggcgg agaaagtcct cggtggcggc aactcggtga aatgcaaagt
    48361 aatcgagatc gaggcgatcc agcgaaaagc cgccgagtac ttcaaggtgg agccgcacat
    48421 gatgcaggcc aagaagaaaa ccgccgatgt agtcctcgcc cgccaggtgg ccatgtacct
    48481 ctgccgctcg ctcacgagca actcgctcaa agtgatcggg gcggccttcg gcggccggga
    48541 ccactccacc gtcatccacg cctgcgacct gatctcgcgc cgcatggccc gggagccgga
    48601 tctccggcac aaaatcgacc aaatctcggc ctcgttgctg tactaacaag atgtggagtg
    48661 aagttgtgga agatgtgcgc acaggtcggc ttggacgcga actgtggaag aagtcggaaa
    48721 gggagaggtt gttgtgggga taacgctggg ataaaaaaag gatggggatt tgttttaaat
    48781 tgttgccatg caaagtgtac gggtgacata ttagtggaag gcgaggggtt gtacacaacc
    48841 ccacagccct tattaatgtt actcgtgaga taagtagaag gatattgtaa gagacaacgt
    48901 ggggataggc tacgttgcag gagagcacgg agatgaaatt caccttaccg aagtcacgac
    48961 tggggcagta cctccaacat atcctccaag tagtgcccgc aaagtcgacc cttccgattt
    49021 tgtcgaatat tctgctcgaa gcgctggaga acaaactgaa agtctccgcc acggatctcg
    49081 atatctcgat taccgcctcc ctcgatgcga ccgtcacgaa aaaaggggcg gccgttgttc
    49141 ctgcgcgtat tctcttcgag atcatcaagg aacttccgga gtcggagatc acggtcgaga
    49201 ccgtagggtc acgggtggaa ctgaaagtcc cgaacggctc gtacaagatc ggttcggtaa
    49261 cgccggagga ctttcccaag ctgccggccg tcaacacgaa aaaggaaata cgcatcgtgg
    49321 cgggcagcct cgtcaacatg atccgtaaaa ccacgttcgc ctgttcggat gatgagacgc
    49381 ggccggccct caacggagtg ctctggcaaa cgaaggggga gaaaatgcag atggtggcca
    49441 ccgatggcca ccgcttggcc aagatggcca cggagaacag acagctgaag gggatgtacg
    49501 aggatgttat cattccaccg aaggtgctga atctcattcc caagtttatc gcaggcgatg
    49561 atcaggagat cgggatcatc ttcggggaga acaacatcat cttcaacctc agcgacatta
    49621 ttctcacttc ccgtctcctt gaggggccct acccgaattt cgagcaggta atcccgcagg
    49681 acaatgacaa gcggcttacg attcacaagg acgaccttgc cggggcggta cgccgggtgg
    49741 cgattctctc caatgctctg acacaccagg taaagttctc cctgaaagac aacacgctgg
    49801 tgctctcgac aaccaacgcc gatgtcagcg gcgaggggaa agagacgctg gagtgcgact
    49861 acaaggggga tgcgatcgag ctcggctaca acgcgaacta catcacggag atcctctcga
    49921 aaattgacgg tgacgaggtg atattcgagc tggccacttc ggtctcggcc ggcatcgtgt
    49981 acagcccgga tgtagcaaaa gaggatttcc tgtgcctcgt aatgccgctg cggctggctg
    50041 actgacgatg aaaggcggat tctgaataat ggcagatggc agcgtctgcc atttttcatt
    50101 ttggtatgat actccgctcc cttcaccttc agggttttcg caacttcgcc ccgctggcga
    50161 tcgaattcgg cgagcgggtc aacattctgt acggcgacaa cggctcgggg aagaccaacc
    50221 tcatcgaggc gatcttcgtg ctctgcctgg gccgttccca ccggggagtg cccgacagcg
    50281 tgatgctgca gcaggggacc gagactttcc gtcttgaggg gcggctcagc cgcaacgggg
    50341 aaacggaggc ggtcgcggtg gcctaccaga ccggcggccg gaagaagatc acggtcgagg
    50401 gagtgccgat tcggccggcc gagctgtacg accggttctg cgcggtcacg gccgggccgg
    50461 aggacacgga acttctcgcg gggtctcccg cggcgcggcg ggtgtttctc gatatttatc
    50521 tcgcgcagta ctccccgagg tacatcgcgc agctaagcga ttacagccgg gcgctggcgc
    50581 agaagaatgc ggcgctgcgc atgaacgcgg cgccggaggc gttcaacgcc gtgctcgtgc
    50641 ctttgggggc ggccatcacg gcggcgcggg cgcgctttct ggagcaactc gtgcccctgg
    50701 cggccggcca ctatgagcgg atttcccacg gctctaagat gacgattgcc taccgggctt
    50761 ctgccgcgag tgtcaccgac agcaacgggg cggaggggat ggccgcgcag tacgagcgca
    50821 agcttgcttc ctacacggag cgggagcggg ccatgcaggt gtcgctcgtg gggccgcacc
    50881 gggatgatct ggcgatcgag atcggaggct acccggcgcg gacgcacgcc tcgcagggag
    50941 agatgcggac ggcggcgatc gcgctcaagc tggccgtgta ccaactgctg gcggcccgac
    51001 ggggggcgct cccgctgttg ctcctggacg aaatattcgc cgagctcgac gacaggcgga
    51061 tcggggccct cctggccgag ctggcggaat tccagcagct ttttctcacg acggccgggc
    51121 agccgccttc ggccctgcag gcgcagagcc gacggtttga gatcagcggc ggacgaatcg
    51181 cgggggtgca gtaatgccgg aactggtgct gcgggagatc cggaaggagt tcgacgggcg
    51241 gtcggtcctg gaggatgtgt cgctcgaact ggccgcgggg gaactgcttg tcctgctggg
    51301 tccgtcgggg tgcggcaaat cgaccctgct ccggctgatc gccggcctgg aggaactcga
    51361 ccggggggag atcctcctgg gggagcggcg gatcgaccgg ctcccgccga aagaccgcaa
    51421 cgtcgccatg gtgtttcaga actactcgct ctacccgcac atgacagtgg agcagaacct
    51481 ggcctttccg ctgaaagtgg cgaaggtgtc gcgggcggag cgcatgcttt tggtggcgcg
    51541 gacggcggcg ctgctggggc tggaagaccg gttgaaactt cggccgggcc agctctcggg
    51601 cggccagcgc cagcgggtgg cgctcgggcg cgccatcatc cgcgagccgg cccttttcct
    51661 actggatgag ccgctgtcga atctcgacgc cgatctccgg gcgcgcatgc gggtggagat
    51721 tgtgggtctc cagaagcggc tggggacgac gatgatccac gtgacccacg accaggccga
    51781 ggcgctcacc atggccgacc ggatcgggct gctccacgaa gggcggctgg tccagctcgg
    51841 gaccccggag cagctctaca acgagccggc gacgctgttc gccgcccagt tcgtcgggtc
    51901 gccgcggatc aacgcggtgc cggtgacggc cgacggcgag gggatctcgg tgttcgggtt
    51961 tgcacggagc gagctggggg cggcgccgcc ggcgggagaa ctcaccgcgg cggtgcggcc
    52021 ggagaaaatc cggatcgaac cggaggggcg ctttgccgcg agcgtcatcg gctgcgagta
    52081 cctcggcgat cagtatgtcg tgacgctgaa gttcggggag acggtgctca cggtggcggg
    52141 cgtgacccgg gaaatgcgcg cgggcgagcc ggcgcggttc gccattgcgc cccgggacct
    52201 cctgtttttc gatcgggcga gcgggcggcg cgtcgggtaa agcggggggc cacggccgcg
    52261 ctttccgggg gaagccggcc gatccggcag ccagggttgc acagtctacc ggaacaggga
    52321 gcgggacggc aggtcaagaa gggcccggaa ggagtagacc cccagagccg cgccgagcgg
    52381 gaagttgaac aggtggagga cggcggccgc ggcggggagg ttgaacacga agatgactat
    52441 gttcagaata ccacggcttt cacgtgcttt tccctcgggc ggcgcgctcc tattcttgcg
    52501 agaggcggtc aagctccgcg acctggtcgg cgaaacgggt gagagtggac tcgatcgccg
    52561 cggggcggct catgtcgaca ctggcgcggc gcaggagctc aatcgggtag tcgctcccgc
    52621 cggcggccag caggtcgagg tagcggttga tcgagtcagg gccgccggcc aggacgttat
    52681 ccagaatcga ctcggaggcg gcgtaggagg tggcgtactg gtagacgtag aaggtcatgt
    52741 agaagtgcgg gatgcggcac cacttgaggc cgaagtaggg atcgtcgccc aggtccgggc
    52801 cgtagtattt tcgggtgagg tcggcccaca gcgccgtgag ccactcgggc gacagggctc
    52861 ccccgccctc aacctgtgcg tggatggcca gctcgaagtg ggcgtagaga acctggttga
    52921 agaaggtccc catggtgttg tcgatgtacc ggttgagcag gtagagccgg gtggtcttgt
    52981 cctgggcctt cgacagcagg tgctgcagga gaagaccttc gttcagagtg gaggcgactt
    53041 cggcgacgaa gattgagtac tgggctttcg ggtagggctg gtgcgtattg gagaggtggg
    53101 agtgcagggc atggcccatc tcgtgggcga gcgtaaacat gttgtcgacc gtggcgttgt
    53161 agttcatgag cacgaacggg tgcacgccgt agctccccca gctgaacgcg ccggaggctt
    53221 tgccggcggt ttcgaagaca tccacccagc gcgaggcgaa cccccggcgg agaacgtcga
    53281 ggtaagtcgg gccgagcggc cggagggcgt cgagaatctc cgcgatcgca tcgtcgtagg
    53341 cgacctcgta gtcggcctcg ggaaagagcg g
//
LOCUS       DAOPOE010000008        53010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_804_length_53010_cov_5.444207, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000008 DAOPOE010000000
VERSION     DAOPOE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 53010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53010
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_804_length_53010_cov_5.444207"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKM94_02205"
     CDS             complement(<1..338)
                     /locus_tag="PKM94_02205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOD65478.1"
                     /translation="MEQLFEGVGPVFWIIATVLLVSGFMVVSLKNIFHSALFLILCLF
                     SVAGIFLLLEAEFLAAAQVLIYVGAVAILIIFAIMLTGNLASRRIRQLNENVLVGFFV
                     SVVFVFAGVLL"
     gene            complement(338..955)
                     /locus_tag="PKM94_02210"
     CDS             complement(338..955)
                     /locus_tag="PKM94_02210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOD65479.1"
                     /translation="MGVLNDLKALLQGLQIAGKHLGRHAVTIQYPEQRDEIPERSRGM
                     VVLLSDKETGELNCTACLLCEKACPTGAITIDAPRGEDKKKQLAQFVVDHTLCCFCGM
                     CEEACNFCAIKLAPNYEYSRRRPADLVFDIAMLQQWGKGVPYEDTRRRKNPVRKIGPA
                     AATEGANTTRDTGGDEDDKPARPAARKAATETTPEDPGRPAEGAE"
     gene            complement(973..2052)
                     /gene="nuoH"
                     /locus_tag="PKM94_02215"
     CDS             complement(973..2052)
                     /gene="nuoH"
                     /locus_tag="PKM94_02215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOD65480.1"
                     /translation="MILPALLALEMFELYRVFFWLGERLHDLGLPGWMIDPVRYGTLG
                     AMMFGVLCLVALFLVWWERKIAGHTQQRFGPMRHGWHGWYQTVMDALKLLQKEDIRVA
                     TRDRAVFFWAPVVGFVAAFLAYIVIPWGDNLIVADLNIGILYIMAVTTFTVISLLMAG
                     WGSNNKYALLGGMRSAAQVVSYEVPMVVSVLTVIIFVGSLSMVDIVTSQAGLVFNWFI
                     FRLPYGPIAFLTFITAATAEANRTPFDIPEAEQELIAGYSTEYSGMKFAMFYLAEFVN
                     MFTVSAVAVTLFFGGWNGWAAVPSWIWFLGKTLVMVLLLMLFRWTYPRLRVDQLMEFA
                     WKFLVPVTFANLIAAGVFKYLGWYW"
     gene            complement(2049..3158)
                     /locus_tag="PKM94_02220"
     CDS             complement(2049..3158)
                     /locus_tag="PKM94_02220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOD65481.1"
                     /translation="MANALRTEEFIVNMGPVHPSTHGVCRLLLTMDGEKVMKVEPVIG
                     YLHRSIEKICENRTFAQCVPLMDRVEYVTAMSCGYMFAGAVEKLGGIEVPPRAQYLRT
                     IMLELNRIASHLLFYGVTAMDIGAYTPFLYGLRERELIIDLFEETCGQRLTYNYIRIG
                     GVSQDIRPDFADGTRKALKIISAKMSDYTGLLNENPIWLRRTKGVGVLPADLAIAYGV
                     SGPNLRASGVNYDIRRNDPYLIYDRLDWNVATRPNGDCFDRYMLRLDEIRQSIRIVNQ
                     ALDGLPEGEIMAKGVTVNYKPPAGEVYHRVENSRGELGAYIQADGGKKPVRVKLRGGS
                     YNALMALPHFATNVLIADLVAIFATLDVIMPEVDR"
     gene            complement(3162..3653)
                     /locus_tag="PKM94_02225"
     CDS             complement(3162..3653)
                     /locus_tag="PKM94_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOD65482.1"
                     /translation="MTREELGNYVAGKFGDRVTRLATGRYDPLFLVNRASDLLEVARA
                     LRDDEQLRMDYLCNAGAIDTGTAAAPEKRFEIHYALASIRHSCRLDLKITLPAEKPEV
                     DSLQAVWPAADWFEREFWELYGIHVRNHGKLGQFLLPDNWDQGFPMRKDWDAPDFIRM
                     PEK"
     gene            complement(3650..4168)
                     /locus_tag="PKM94_02230"
     CDS             complement(3650..4168)
                     /locus_tag="PKM94_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOD65483.1"
                     /translation="MGVIRKLPVYLEHIPGGSLVLTNLETVLHQSQAKSLWYLLFGTA
                     CCAIELMCTGASHYDFDRLGMIFRSTPRQADLLIAAGTITKKMAPRLRRLYDQMAEPR
                     YVIAMGGCTIKGGPFYYDCYAVEKGIDHIIPVDIYIPGCPPRPESLLEGCLHLQEKIK
                     KQRLLDWQEKPA"
     gene            complement(4156..4512)
                     /locus_tag="PKM94_02235"
     CDS             complement(4156..4512)
                     /locus_tag="PKM94_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOD65484.1"
                     /translation="MSEYLAILMFIAAGIAIVLITFFVARLVRPHHPYPAKDMNYECA
                     EEPIGGAWIQFNNRFYIFALIFVLFDVEVVFLFPWAVAFGQLGLFALVEMVVFILILF
                     FGLYYAWRKGALKWVS"
     gene            complement(4815..6047)
                     /locus_tag="PKM94_02240"
     CDS             complement(4815..6047)
                     /locus_tag="PKM94_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD65485.1"
                     /translation="MKTLALLIGLGATVIPGIAAAQESACVACHQSFEAEDGPSFAEA
                     RSVHGQNGISCHDCHGGDPALDDMDEVRRAKNWRGVPGHLEIPEFCARCHSDPAYMHE
                     HNPGLPTDQLAKYRTSVHGQRLFAQKDGKVADCVACHGAHDIGDAAMPHSRTHPQNIP
                     ATCGKCHADAAYMAEYGIATDQVADYTASVHSQALLDKGDLGAPACNDCHGNHGAAPP
                     GVASLSAVCGTCHAFQMELFNQSPHAPAFAAAGFPMCETCHGNHRIEHPTDEWVGTDE
                     PAVCIQCHSGDDGTRAFAVAAAISHNLDSLRTGYDSAQAVVARARGLGMATTESAFGL
                     QDIRQALIQLRTTVHALSAEQAEAKAAEGFTLISATIGAGERLVDEYYFRRKGLGLAT
                     LCITVLAVALWLKIRRLR"
     gene            complement(6057..7178)
                     /locus_tag="PKM94_02245"
     CDS             complement(6057..7178)
                     /locus_tag="PKM94_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOD65486.1"
                     /translation="MGGGSGPKARGGRVYRWLDERYEIENLVAYMGHKQVPQHRASFW
                     YYFGGISLFLFSVQVVTGLLLLMYYRPGADSAYESVQYIVAEVPFGWLMRSIHSWSAN
                     LMILAVFIHMFSVFFSASYRKPRELTWLSGIALLGLALAFGFSGYLLPWNELAYFATR
                     VGTGMAGAVPLIGGPLLTIMRGGEEVTGATIGRFFGLHVAILPGITTVVLAAHLIFIQ
                     RQGMSEPLAWQALPAEARRYEKFFPHFLLKDLLVWLIVLNILAVLAVLFPDGIGVVHW
                     PLGEKADPLAPAPAAIKPEWYFMFAFQALKFLPAHIGFIEGELFGILVFTAGGVIWAL
                     IPFLDRPSARGGRSPKALAFGAAVAAFMIVMTILGYLLE"
     gene            complement(7178..7630)
                     /locus_tag="PKM94_02250"
     CDS             complement(7178..7630)
                     /locus_tag="PKM94_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOD65487.1"
                     /translation="MAEKNTDRRGFLNLILGGSALATLGAIIYPIIKYITPPAVAETD
                     VALKKLEFTQADLEAAEQKQKYFKFGRDLGIVFVTDKGEVRACAATCTHLDCTVQNRP
                     DEGILWCACHNGKYSYDGRNISGPPPRPLAPYRVNIIDGEIIVSKEEA"
     gene            complement(7823..8206)
                     /locus_tag="PKM94_02255"
     CDS             complement(7823..8206)
                     /locus_tag="PKM94_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOD65488.1"
                     /translation="MRKAIKSSRAPKAIGPYSPAIKVAAGTMIFCSGQIPLDPETGTV
                     VGKTAAEQCKQVMENLLGLLADAGVDFSTVVKTTIYLTDMNDFAAVNEVYGSYFEANP
                     PARATVQVARLPKDVKVEIDAIAVV"
     gene            8471..10405
                     /locus_tag="PKM94_02260"
     CDS             8471..10405
                     /locus_tag="PKM94_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65489.1"
                     /translation="MKRLATLLVLCLAAAMVAAPAAAAALDRAYARHADTWLDFPAFY
                     AGWDSDDSARCLGASHALHPAVVFSPRGFLGYRFWMAYTPICISESDENPHLAVSNDG
                     REWAEFTAASGCLANPVFDRSLFRAIHLSDADLLLEGDSILYLLFRATWEIAGVDSNA
                     LYASATVDGLVWTPPVKILSDGLLGQPRQSAFVSPALASEGDSVFILWVVEPRADGVS
                     SLDTSRMVEYRAPHPFGPWALVDTCAVALPSDSLKIWHLEVLADTLFGRTALLNLSPN
                     SGLNGGDSADLYMAAYDEAAALWTVAAEPVLTWRSDTSAWYGRRIYRASGVWMTTPAD
                     TILWLYYSAYARRANAGGAGTGWQTGLTWAAFDSALGPFCLAPSLRHEPNLEHVVGLS
                     PTFQWLSADPALHRPQDEVSLELIDCTGGDSVAVWSVCVSGGGGAFCPDSFELSPGHT
                     CVLRLRSRHGAAWSPWTALEFRTNAAPSPPACLYPPDQPALPLYDLTPAFFWSEAIDP
                     DPGDPVRYRFALCIADTCADSTDIVTDGISLTLADSLSVATAYRWTVASCDTFGACCP
                     AGPPAQFRTFLPGDMTADFRLGIADLTGLVDILFRGGSLSVPMIGAEMDGRCGLTVAD
                     LTVLMAHLFRGGAPPPPGCR"
     gene            10525..10809
                     /locus_tag="PKM94_02265"
     CDS             10525..10809
                     /locus_tag="PKM94_02265"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOD65490.1"
                     /translation="MPTFIMAMSIVPSAKKTHPELSHDINRSLEVFHANKVKVHSLYA
                     TLGRYDYIAVFDCDDQTCAFRVATGINDLGVLDTETWPVIPYEEFTQLLQ"
     gene            complement(12385..13365)
                     /locus_tag="PKM94_02270"
     CDS             complement(12385..13365)
                     /locus_tag="PKM94_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD65491.1"
                     /translation="MASGTAKITVTPQYLPILLDSLRVDSVLDFDLYLTVNGNMVLYR
                     SADLPFTDRTRRTLLDNRVDRLFITDDNRSRYQRYIERNLPKIMTDPGIREEKKANIL
                     YDTSTNLVKEVLANPTYGENIQRTKDLVTNTVEFILQGKEAFHTLLKITSFDYYTYTH
                     SVNVCTFSLALAQQLGYGDETFLNELGVGALLHDVGKSRVSERILNKRAPLNPTEFEI
                     MKRHPQWGAEILEETDQIAAACYYPVLQHHERGDRRGYPNGLRLDEMHVYSQIVAIAD
                     SFDAMTTERVYQKAMDTFPALKIMFSLQTAYRDDFLKAFVELMGPTGLAD"
     gene            complement(13531..14151)
                     /locus_tag="PKM94_02275"
     CDS             complement(13531..14151)
                     /locus_tag="PKM94_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65492.1"
                     /translation="MLDNRYRRGFSQERLAAIQEPRVRKDSRGYFLMSLSENTKVYFE
                     DFYRFLERTYAKARAERSRLDERLFTTAPEQSELIAYYRARGVIVDLLMQTIIRFYTD
                     GGNFGVVMSPWCFGTVVLEKLEVYRERLSRGEVEDPNIPDYPYYTIRYLDEIHKAALL
                     ELFDFPEKAFQMRWQYSELLKRYSQILSDITTSLNSVLGTIKNYGS"
     gene            14491..14700
                     /locus_tag="PKM94_02280"
     CDS             14491..14700
                     /locus_tag="PKM94_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65493.1"
                     /translation="MRDEAQRGKHTSSRMKRKGRPGQRPARHFRGGSDEAGRRPDRRR
                     QLEQLAETEAEEMLRQDESDEFSPS"
     gene            14901..17024
                     /locus_tag="PKM94_02285"
     CDS             14901..17024
                     /locus_tag="PKM94_02285"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOD65494.1"
                     /translation="MPDLHCASAAAITLAFLALVIAGCSDDETTGPGGGGGGAWTLVS
                     PTTEGNLYRGACFVDATTGWLVGVDGRIVKTTDGGTTWIEQASQTNRTLRAVDFVSRQ
                     DGWVVGDDGRVRRTEDGGTTWSLPLLAVTDRSLSAVEGVTARRVFAVGEEGILYYSLD
                     AGRRFSAVPLEDAGPLRDIVVFDTSAVAVGDDGFIARFFNYDWEIERLTADTTVDTLP
                     VDPDTLPDDFPADTVIDTTFVPGDTIVDTTYWWPDTLVDTTYFTVDTIWSGFETVTAE
                     VDDNLLGVAYAGGNSLWAVGENGVFLSSDDRGASWEVVDLGVTAHLTSVASDGAGGGI
                     ITGLNGTVLVTSDNGATWAAAQSGYALDLYASSSPAADNFWASGDAAVFRSADAGETW
                     EQRVTGTSLLPTLTDIVFVSPDSGFAVGYTGTILRTTDAGQTWVTVRQGSAFAPAEWF
                     SAAAFTDNLHVWAVGRTGLPPYYLLVVRTSDGGESWERFEFPDNPPDPDNPAPDTAWG
                     KNVVFLDDLTGYICAGDSILYTTTGGASWVKQASPDTVTPPRTIHDLSFADATHAWAV
                     GKNGLILQTTDGLTWEIPTDTVKTTLWAVDCFDANGVVAVGNGGEIWRTTDGGTTWSK
                     VSSPTKSTLIDVSFVDAQSGKAVGQNGTVLSTGDGGRTWTVQASPTAETLIGVEMIDR
                     NVGWIVGGHAPDASGVMLRTTTGGN"
     gene            complement(17134..18210)
                     /locus_tag="PKM94_02290"
     CDS             complement(17134..18210)
                     /locus_tag="PKM94_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65495.1"
                     /translation="MKGIFAVLAALVLAAGCVKEVDRSAAPKTAADTLTYATHFRGER
                     HLDRIRQLTFGGQNAEAYFNADATRLCFQSMRDTMSCDQIFTMNVDGSEVRMVSTGKG
                     ATTCSFLSPDGRHIIYASTHLGGDTCPPKPDMSQGYVWALHETYDIFRANADGSDPVR
                     LTDTPGYDAECVYSPKGDKILFTSVRSGDLELYMMDPDGGRVEQLTHAPGYDGGGFFS
                     ADGEWIVWRASRPEGEALADYQRLLAQGLIRPGKLEIYVMNLADRTPIQVTDNGAANF
                     APYFHPDGRRVIFCSNLADPGGRNFDLFMVEVATKTVERITYNETFDGFPMFSRDGRT
                     LVFCSNRDNAVPGETNVFIADWKD"
     gene            18295..20496
                     /locus_tag="PKM94_02295"
     CDS             18295..20496
                     /locus_tag="PKM94_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65496.1"
                     /translation="MRITLFVMVLSLAIGTAAGESETDVLGRILGEVGFTRADLGYHP
                     AGYWNRFPLDVPYRLTSFDALFAEPLKLYDYATTMGNAVELYLDPTYADTASDGLYQL
                     VYNLGVDRKLGGFRSYSANLRPAPAGEEPLVDAVEGLYALAGRDTEFYTFGNRAESPD
                     MTAEIRQKTARLPDSVQVVLAGLIVNLTDAIRWRATALRNCDVADLEAALAIRDLAST
                     QGDGMVYYPELDDIAAAIDLPSMHYAALKTAAAAESAERALRRFARAVPDDFTAELPT
                     PFGTIALFSSVARSERMAPPRLFGELRASLPQWTEYDATNTLLVVDFGRRMIYQGTPG
                     AAASLGNPVSVVIDLGGDDYYGRERAQYPPAAGVGLCGVGLVIDSEGDDRYEGSTYAQ
                     GAGLFGVGVLLDRAGNDEYRAELSSQGAGYFGIGLCLDGVGDDTYYLCGDGQGMGGVG
                     GGVGVCASFGGDDRYTAEPLAEKYNRGDYHSQNKINGNNAQGAGFGRRGDGSDGHAWA
                     GGLGALIDINGDDRYYSGNWTLGIGYWFATGIAYDKRGDDLYESCYFTQGSGAHYCNG
                     ILIDEGGNDRHELYETAGAGLGFGWDFTNALLVNKGGNDVYRAKMISMGLAQIRSNAF
                     LIDIGGDDAYYLGQGTPGLGEATFREGYDRPGRLTTYYAYASSFGGFIDIGGRDRYVS
                     FTDSGETTHPWAADDTLWFRPARTDSLFGHNNYGVGVDAEAGAVPELEKWK"
     gene            complement(20895..24335)
                     /gene="mfd"
                     /locus_tag="PKM94_02300"
     CDS             complement(20895..24335)
                     /gene="mfd"
                     /locus_tag="PKM94_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOD65497.1"
                     /translation="MTTGTDRSHTTHSRLRQVIGRWLEYAPARELAARLEEGPAEPVP
                     IAGLAGSAPAFLLRALTDTLGRPVLAVTVTSEQAADLADDLAVLLGPDRVGHYPARQV
                     LPYDFKAPQGEVMGRRISSLADLMDNRLAAVVAPLRAVLEPTIARDHLEKSRVRLEVG
                     RAADLDDLVRRLVALGFRRVPVVEEVGDFALRGGLVDFFSPGSTAPSRVEFFGDDVDT
                     IRLFDVGSQRTIGRVDRVNLLPRREVPITMETVEQYLAALPEHDADYIRDRYLNDPEL
                     PGLEWLAILFGVRPGSLREYLPPDTVVWLEGEHLLRDEAEGILAEGRGLYQRLKGRLE
                     KLPAPEAYYHSVEGAFAWLAAHPRIDHAPFRGGRRDVIDFGCRPHPSLGSRLDLLAET
                     AREFDALGMVHFVATDTESQAERLDELIGERLGPESRPVIEPADLKGGFVCPAGRFAV
                     LTDHEIFGRHHRRVRRKKFREGVAISDYSALNRGDFVVHTDHGIAKYLGLETLVVDGR
                     NRDCLALLYEDNDKLYVPIEEFNRVSKYAGKDTAPQLTRLGGPQWDKLKVRTKKAIAD
                     MAADLLKLYARRRSAPGFSFGEDTTWLRQLETSFRYEETPDQLKAIADVKRDMAREQP
                     MDRLIVGDVGYGKTEVAVRAAFKAIERGKQVAVLVPTTILAQQHFQTFTERLAEFPMR
                     VEMLSRFRTAKQQRAVVEDLAAGRVDLVIGTHRLLSSDIFIPNIGLLIVDEEHRFGVR
                     HKEKLRHLKAAVDTLAMSATPIPRTLHMSLMSVRDMSIINTSPKDRLPIITEIVEFDP
                     AIIATAILREIGRGGQVFFVHNRVQTIEAMHHYLKKIVPQAEIAIAHGQMHERSLEGI
                     MLAFLARRYNVLLCTSIIESGLDIPNANTIIINRADRFGLAQLYQMRGRVGRSATRAY
                     AYLMTPPVKQLQPEAIKRLRALEAHSDLGAGFALAMRDLEIRGAGTLLGPRQSGYIEE
                     IGFDMYNKLLEEAIAELKGEELVRPPDTKLELDLELHLPDEYINDRQQKVDIYRRLAD
                     GRTLDDVERIRDEVTDRFGAQPQSAVNLFDATAVKILAGAMKVEKVKMAAGRVSLFLE
                     EGRQLTRRTVEALRQGADCPMEFSMIGRVQIMLDLGRIGERDRLPYLRKVLSGVEPGG
                     "
     gene            complement(24479..25333)
                     /locus_tag="PKM94_02305"
     CDS             complement(24479..25333)
                     /locus_tag="PKM94_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOD65498.1"
                     /translation="MADEQPIIIRRKKHVHGGHHGGAWKVAFADFMTAMMAFFLVMWL
                     VGQKPEVREAVGGYFRDPGKYLEQGGAGVLKGSRSAITEKDTPSGMLKNESDKSGGGP
                     SEMEKKQMKEAALKIISQLEQQEAFHRLRDNVKMQLTSEGLRIILNESAESPAFFEPG
                     SAKLLRQSAVILMTIAGELGHLQNRIVLEGHTDAAYTGDLLYTNWELSADRANAAREL
                     LEAHGLWDGQVREVRGFAEQFPMIADNPADARNRRVTIVVLYEHASMAYDQMEVGADL
                     MALAEAQE"
     gene            complement(25342..26199)
                     /gene="motA"
                     /locus_tag="PKM94_02310"
     CDS             complement(25342..26199)
                     /gene="motA"
                     /locus_tag="PKM94_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOD65499.1"
                     /translation="MFIFIGALIVLGGVLGGYTIHGGKILALNQPSELLIIGGAALGS
                     LLISTPLPVVRGIFAQLKQALGGGLKKKDYLSLLVMMFEIFNVARKDGLIGLESHVEH
                     PEESEIFKRYPGFLKNHHAVAFFTDTMRVIISGAVQPHDLEDLMDADIETLHEEELRP
                     STALGAIADALPGLGIVAAVLGVVITMAAIDGPPEVIGHKVAAALVGTFLGILSAYGF
                     VGPLSSSLKHRTNDMRQYLGCMKHALLSFHKGVAGVIAVEFARRSLYAEVRPAFLELE
                     KACNETKKR"
     gene            26430..27215
                     /locus_tag="PKM94_02315"
     CDS             26430..27215
                     /locus_tag="PKM94_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOD65500.1"
                     /translation="MRFFGAVLPPRRGLAAGRLAGFVGLAALLAGLLLPRASLSQPTI
                     DLSRQRMPQAMPGRGANPNPSALKVARLHYSGGGDWYWGSSALPNFLTYLNRQTDFPV
                     DTMERVVTIMDADLFRYPFLFATGHGVIRFEAEERERLRRYLEGGGFLFVNDSYGMEE
                     TFKKEMAELFPEREPVAVPFDHPIYRCFYDFPNGPPKIHEHDGQPPRAWAIILHGRIV
                     LYFLVESDIGDGWEDPQVHNDPPELREQAFRMGVNLVTYALLY"
     gene            27326..30730
                     /locus_tag="PKM94_02320"
     CDS             27326..30730
                     /locus_tag="PKM94_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65501.1"
                     /translation="MTELTSRRALLAKLTGVLVRQRAVLFVSGVMLTAAAVTAAAIGL
                     SLWANVMILPVAVKLPVLIAVGAGTLYLFGRYAVARLFSGSIDAVAVALEERNPELKG
                     RLIAAIQFARMKDAGCYSAALIEATERQALARAQGVNFNQALSFYPVLRSGRYFATAA
                     AAALLVVALLPGMFRYSFLVFSNPTTEIAPPLAYKVVPFPGTTEWVKYRDIEIGASIF
                     GERLPESAVIHHRLVDGNWQETEIDLRKVRRAPMVNGDSVRIATTLRQINRSFDYYVE
                     AGRVATPVQQVSVVDRPRVNGLKLSIFYPDYTGLAPTVIDENNGSFSAVVGSRVNLAV
                     ETNLPVARAELVFDDSSRTPLEVDGRQAETALRVDKSLGYHVRLIDGLGEENPDPIQY
                     HITAVPDEYPSIDVLFPGVNVNLSDEMVLPLKVRIFDDYGFTSLVLKYTVVAHGQASE
                     ENVAVLHFSDRIKTEGEIAFNWDMDKLNMYPGDYTIYRFEVADNDVNSGPKVTASRQY
                     VARIPSLEEIIAEAEGESSRRVDNTRQLLQQGEDLSERFKNMARKLKAQNRDEMKAEW
                     QHQKEMEALAAQNQELIENIEKMAEEMDKSVEKLADNAQMSREIVEKLQQIQKLFEEV
                     ATPEMKEAQKKLMEALQKMDRNEIQKAMEQFQLSQEEMLQRLERTLALLKQMQVEQKM
                     QAMMRQAERLLEQQNQVNQATDSSAQSALPPLAERETDLKKDLESLKKETADLRKLMD
                     EAQMNQSEEARKFAEAVEQTDADRNMEKMSGALAQQQQQPAAAEGKQASAKLAQMLSS
                     MQQQLAAMNSGQNEALRKMMRLAIDDANYLSQSQEELLKRAAEVTTQSDILRELAQSQ
                     QDLMTACSGLKGRIAELAKESPFVASELGQIMNQATAQMDMAMLGLGDRQGPQAIALQ
                     RDAMTNLNKVSLYLMEAMNQQNQCDKGGSCDKNMAKLQSMCNKQNQVNQETQQTCNNP
                     SFNPASQGQQTREALQRLAGEQGAIRKSMEELAQEFGDSRQVLGRLEDIADEMREIEE
                     ALAEGEAGPETIERQLRVYSRMLQASRSLQRKDFTEQRKAVTAEEQIYQVPPALPAEL
                     LNDRTNLEDRLRQYLGEHYPRQYEEQVKAYFKALLQTEAAPQEGGR"
     gene            30727..32598
                     /locus_tag="PKM94_02325"
     CDS             30727..32598
                     /locus_tag="PKM94_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65502.1"
                     /translation="MKRTLGRLIAALALAAGLLGAALAQAAEEPKEVTRPPGGLRLYT
                     PQDQQAQDQQMLNMVREMMRSKNYEGAVGLLEAIDRRDRGNMVVMNLLRTCYDQLGQP
                     GSSEVVIRRMLEITPNSHVLHVYLAEALVRQEDTAAGRQEYATALELVPEKTNPGMLS
                     QVVVSMQEVGLSRDVLALIERLRRELGSPRLFAKEAGEIFEAREEYRRAAEEFYAATA
                     DTSRTGIEAETKLQMMLQFPKSAPAALGVLREHTADTLATAKAAQILAGHYLQAGDMD
                     QAMHYTLLRDSLAGTNGTVLLWFMHICSERGLYAQTTVVGAEILRRYGASPLVAEARY
                     LYAEALTRLGRYAEAVAVYDSIVAAYPRVQDKAEAACRIGQIYLNYLNEPRRALRLFD
                     SVAAAYPGGLGYLRALLAAPDAYLRLGELDSARTALGRLAQRPLIEEAAEEAAFKLAK
                     IDFYEKRFDTARAGFNKLLVAYPKGFYVNDALQMMLLIDEAGSEPLLSEFSDALLFEE
                     RREYDSCRAELERIAGADHPALSDDALYRLADLSIARFDSAAALEYIERLAGQYPESY
                     FAPWGLKLKGDMLTADPRRREEGKAIYRRLLEENANYPFTSDVRKRLRELEVDPRTG"
     gene            complement(32617..32865)
                     /locus_tag="PKM94_02330"
     CDS             complement(32617..32865)
                     /locus_tag="PKM94_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65503.1"
                     /translation="MFVWCSLLFVLGILAFLDSVFNMGMIFRQVNSVFFMLVSLALLV
                     RTTTKMKEKKVESYQDKVFQLERRVRTLEEGRKKLETY"
     gene            33022..35790
                     /locus_tag="PKM94_02335"
     CDS             33022..35790
                     /locus_tag="PKM94_02335"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD65504.1"
                     /translation="MADLSLTDPDKFDRTNAVAAGAVFLVSFITYALTVQPTFSFWDC
                     GEFIATSYILGIPHPPGTPLFILLGRIFSVIPFVEDISHRINYISVITSAFTAMFSYL
                     LTVRLVQYFFGEQRRELLNRVISYVGGVVGGLFVAFSRTNWGNSVEAEVYGAALALSV
                     MIIWLAVQYHDARGTSKGIRLLILIFYLAMLGIGLHMTVFLVVPVAAFFLLFNRDAVP
                     RDYAYLAAFVGLELLLIVLFSGDRGGPVAFYLTTVLLAALLVVLLYRKINWAILIALA
                     AVSTVMISFSLFMEALPFFVVLMIGLAVAAHRRGWRFQWLTGLGLIVVAVLGISVHAF
                     IPIRSAHNPRIDENNPSRNWSTFVKFLDRKQYGSESMVDRMFHRRGTWENQFGHHPHM
                     GFFSYFEEQYSPKDWGFVPFLGLGLLGVIVAVRKRQEIGLPYLVLLVVTSAGLILYMN
                     FADGTQYDPRTGDAYLEVRDRDYFFTPAFVFFGIAIGMGVSALMMLVREALARRNPAI
                     ERTAVYAMTALVLLPAVPLAHNYHANDRSDNYLPYNYAANLLDSVDKNAILFTSGDND
                     TFPLWAIQEVYNYRKDVRVVNLSLLNTDWYVYQMKHFYNVPISLEDDQILWEDVEVRP
                     GLTLPMPVKEFRDRPRNRTTWLAAVEPYNGQMVKVQDMMVDEIVVENRWKDPIFFSSQ
                     PYDSPLDLQKRTAAVGVVYRLDRTPPERLIDAGRGYDLFMHTYRFQGYEDSKVYRDEN
                     ATGVFIGYGINAVRLWDEFEKRGQLDSAMAIADKIMKEYPEYAQMYMIVSNELRQQGD
                     TAAADSLLRQAHDTLTAFCRSNPENLFYLSDLGLIKVSIGSISGRQDLLDDGLALMWE
                     AFRANPESGTAFRKLFYALSDQRRYAEIQEAARLHASYPRNLEDPFLQQLLGLTQSTP
                     RIPPGAGQ"
     gene            complement(35867..36349)
                     /locus_tag="PKM94_02340"
     CDS             complement(35867..36349)
                     /locus_tag="PKM94_02340"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HOD65505.1"
                     /translation="MDYYEGVIKNTEFFTTTELADKLKMNVQVITRKVQAGEITAYKI
                     GKDWRIPEQSVYEWLQQRSNRNGAAAAPAPRRNIRLKRSAETETVPPSRRTKRKYLLE
                     YILAQFEPNRLYEDHEVNRVISRFNEDPVAVRTDFLEEHMMEQVGGRLRRSAGYKLSE
                     "
     gene            36856..38043
                     /locus_tag="PKM94_02345"
     CDS             36856..38043
                     /locus_tag="PKM94_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOD65506.1"
                     /translation="MAAMQMGARAPGQQGKRTRVKYILSIDKLTQLSEAERTALKEVA
                     RRYAFRTNDYYLGLIDWSDPHDPIRRLVIPAVGELRQWGHLDASREGKVTVRKGVQHK
                     YSSTVLLLVNEVCGSFCRYCFRKRLFMNGNDEAAYDIRPGIEYLRTRPEVNNVLLSGG
                     DPMILSTPQLDGILTALRSIAHIRIIRIGSKMPAFNPYRFLDDDELFAMLRRHSQPDR
                     RIYLVCHFDHPRELTPEAREAVRRAIEAGLICVNQNPIIRGVSDDPEVMAELWNELSY
                     MGIPQYYVFQGRPTAGNEPYEVPLVEAYCSIERAKRKCSGLAKRIKFCMSHESGKVEI
                     IGVDHRHVYLKYHRAKHYRDEQRLLVCHRNDQAYWLDQLKPVEGYRNEYYSDQGLRLY
                     GFS"
     gene            38246..40549
                     /locus_tag="PKM94_02350"
     CDS             38246..40549
                     /locus_tag="PKM94_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65507.1"
                     /translation="MRRGLVSWAALLCAGIIAGRAAAVPPTISYQGRLTDGGAPVPDN
                     TYEIVFRIYAADAGGSPLWLETQEVATADGLFTVLLGSVAPIGEGVFDGSVRYLTMQQ
                     EGAAESAPRIPIVSVPYAMTAGSVEGAAADCSDCDTIFLNAIGPDELRAAVDTALKVT
                     DTGAAARVGLRARLETSTTDTVSAIRGIAYNGGSGRTQAASFDATGEGGGPVHGINVQ
                     ATGNGSSYTTMGVTAQATNDAAGTARGGYFAATNDGFGDHVGAIGQSYSPNTSAAKSI
                     GLWGNARHYGIGRCVGGMFAAADGANTFGVDASATSANADSTCAVRGYAKNSATGGTS
                     DAIGGVFIADSAGTAWKWGSIGLAYGGSREATGVYGWAETNATVSRATGVFGQASNTG
                     PGDTQGGYFWGHGESGDTYGAKTFGYSTTGSVSGLYAHGSVTDNVYPVYGVYGRVDNY
                     SSGSFNSEAYAGYFENCVYNASNAYGLYGHVYDVQGIYACYGVVGRTAHTGSGSSIAG
                     SFETDSTGTGTAYGVRASGLSKGSAPAYGVYGSVASRGNGNLYAGYFKVSDYGTGLRY
                     GVYASSSVSGYAGYFSGDVRVTGDQVVVGSKSAAVEVEPEDYRLVYSQESPECWFEDF
                     GEGQLVNGRTHVELDPVFLRTVTVSAQHPMKVFIQLNDPDCNGTAVVRGMTGFDVIEL
                     QNGQGSASFTYRVVAKRKGYEDVRLAQMPGQTPEEVEAEAKQAEARLQTEEEQAAQRE
                     IAERDRLAQIRQEERERQEARPFGDRD"
     gene            40794..42494
                     /locus_tag="PKM94_02355"
     CDS             40794..42494
                     /locus_tag="PKM94_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD65508.1"
                     /translation="MNQRRCRFAYAETISALLAVIFAVAPVFAQQSTTGPEPPPGIDR
                     GLAPPVRGTVVLDVTAYLWHHGCGPTAAGMVMGYWSMNGYEQLVKYGVPAMIAGDHGD
                     SVCGAPIGDHYHDYSCPIDGGSPIPDMSELGGAHTSDCIADFMRTSWSAAYNLYGWSW
                     FVDVGQALLAYPPYADPTYTHFRMRELDFSSVSFTDYKAEIDAGRPTVFLVDTDGNGV
                     TDHFVTGIGYSEDPPKYGVHDTWDQSIHWYLWRAIAPGVSWGIYGMHFFGPAVSFSAA
                     PRFGDISLEVQFTGQSFDPPDSWQWDFGDGQSSSVRSPSHQFTAPGIYDVSLTATFAG
                     EPISSLREDFIIALADSLAGQDQLVEGGSEVEVVISASNTVPLKEILIPLSYAGTLDL
                     TLVSFSTAGCRTDYFESTGYFDFAPGRDQATIYLISSTAGTSPELAGGSGPVVRLTFS
                     TGSAGPGDSAVISMSGYSTYSPRFSGSLLAYTPKTLSPVLRYAECCVGERGDVDGDGS
                     VKISDVTALVSYFFRDGEPPACNDEADSDGSGVLNIADLTYLIGYLFRGGPVPALCPR
                     "
     gene            complement(42627..43388)
                     /locus_tag="PKM94_02360"
     CDS             complement(42627..43388)
                     /locus_tag="PKM94_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD65509.1"
                     /translation="MSQDLTGKIVLVTGASSGIGEACARKFAARHARLVLAARRVDRL
                     RQLVSRLGTDVHVLELDVRDRTAVNAAIAGLPHPWSEIDILVNNAGLSRGLDKLHEGD
                     YRDWEEMIDTNVKGLLWVSRAVIPGMVARRRGHLINIGSIAGREVYPNGNVYCATKFA
                     VDALTKGLRLDLVDTPIRVSTVDPGAVRTEFSEVRFHGDTERAAKVYQGFTPLSGDDV
                     AEAVVWVADRPPHVTVADVLILPSAQASANLIHRE"
     gene            43731..43889
                     /locus_tag="PKM94_02365"
     CDS             43731..43889
                     /locus_tag="PKM94_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65510.1"
                     /translation="MTQNSTSAKKRSVRNGLPKKTYWHLYSLQSLESDKLAKIVNKIL
                     SREYVLIK"
     gene            44017..44922
                     /locus_tag="PKM94_02370"
     CDS             44017..44922
                     /locus_tag="PKM94_02370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD65511.1"
                     /translation="MFKKKLTFMLIPHSMGISRQVRIPVAAIYLAVGGVMLLVFASFF
                     LSAEFFADKVDARELARLRAENAALHRQFEQLKWNLTEVETRFSQLVDREVKIRSLFS
                     LPEIDPAERQLGVGGPAPPSAAMMSNVQREATVTGQQIDRLLTLSSFELEKFNEVEDE
                     LLKLRDRLDHTPSIWPTNGWLSRGFGMKFDPFTGMKQMHRGLDIANRTGTKVIAPAAG
                     RVKFVGQNGGMGNTVVIDHGYGFETRYAHLSDFRVKRGQRVERGDVVGLMGSTGYSTG
                     PHLHYEVLRNGKHLDPRVFILNDNS"
     gene            45080..45718
                     /locus_tag="PKM94_02375"
     CDS             45080..45718
                     /locus_tag="PKM94_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65512.1"
                     /translation="MKRNPAIPHHLREAIPPPDERDEERVKELIREIKRGNQLAFAEL
                     VRRYRVQVAALAYKMVGDYDEAADIAQNVFVKMSRNIWRYDERRKFYTWLYRITVNAS
                     IDYIRKHSRHRHEPLENFENVVEQADRRPDASYHRRQIDDYIREATASLTDKQRSAFV
                     LRDVEGCRIDDVADIMNMPEATVRWYLHRARTKIRRELLRKCPHLLALFGIR"
     gene            45815..48022
                     /locus_tag="PKM94_02380"
     CDS             45815..48022
                     /locus_tag="PKM94_02380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOD65513.1"
                     /translation="MAQTEELQVNIDGMHCAGCAANIERGLGALAGVAECRVNLATRS
                     AVVAYDRDETSAGAILRTIEKLGYGARVGAPDVLTANRKELSAAIRRFWPALGLSLPL
                     MALAMGPMVGLGGILAPAADGIAQALLAGGILFGAGRAILGDAFAQARRRAANMNTLI
                     ALGTLTAYGWSLYALIANLAKGGAEPLFFDSAGMIITLILLGRLLEAKSKGRAGEAIR
                     SLVQLQPAKTTAIIGGTEVEIDAAAAQPGMLLRVRPGERIAADGRVVEGTPAVDESML
                     TGESLPVEKQPGDAVIGGSLNGHSVFTFQVTAAGADSVLAKIIALVSEAQGKKAPVQK
                     LADRVAAVFVPIVLGLAALTLALWLLFAPGNDVMMRSVISVLIIACPCALGLATPTAI
                     LVGTGRAAREGIIIRGGDVLERITTVDTVLFDKTGTLTHGELQATGVTALNGYREEEL
                     IALVGGAESMSEHPIGRALARLREERGIPPAEVRKVEARPGFGLIGQSEGGRLVIGNQ
                     ALLEQENVALAGHQEAIERTTAAGHTAVLAGLDGKLIGLFGIADRVRPEAAEVVRELQ
                     KTKQVAMISGDTRRTAEKVARLAGVEHFEAEIKPDQKRLLVDSYRRAGGTVAMVGDGI
                     NDAPALAAADVGVAVGSGTDVAVETADVVLVRPDLRGVPKMFLAAAWTMRVIRQNLFW
                     AFAYNVLAIPIAAGALYPAFGLTLTPMIAAAAMAFSSVFVVLNSLRLNRVRFR"
     gene            48222..49226
                     /locus_tag="PKM94_02385"
     CDS             48222..49226
                     /locus_tag="PKM94_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65514.1"
                     /translation="MARLHLTPKGALIAFIVLAATAVAQMVWWIILMAHLVDAQVEVA
                     RRVGADPVLIEQLHREEVRRQVMVGLEGVFFLVLIIGGAWLIYRSLVRTEQLKFHQQN
                     FLMAVTHELKTPLASIKLYIDTLKSPKIPEEKKAGVLPRMREDLDRLERLVDNILEAG
                     RFERSGYKLHRQPTDLGRLVERRLGALERTPKHKPLHVTLDLAAGVIVNVDCSALERA
                     IDAVLENSLKYNDKAAVDLAVSLARREGAVHLVFADNGIGLERGDLNAIFERFYRVGS
                     EVSRRHPGSGLGLYLAREFVRAHGGEMAAESEGPGLGARFVITMETEPENEEDPAGGG
                     "
     gene            49198..49896
                     /locus_tag="PKM94_02390"
     CDS             49198..49896
                     /locus_tag="PKM94_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD65515.1"
                     /translation="MKKILLVEDDPNLADGLVMNLEAEGYEVVSIANGSEALDEFRRG
                     SFDIVLLDIMLPGMDGLAICKRLRAEGYRVPILFITARGQTEDRIEGLVAGGDDYITK
                     PFDVAELLARVQGIFRRQAWLAAGDDQLGEVYEFDGRRINFKTFEATGPGGMAPLTHK
                     ECMVMKYLIERAGEVVSRDQLLDAVWGYHTFPTNRTIDNFILKLRKVLEDDPKNPRYI
                     ETIRGVGYRFSRRN"
     gene            49962..50666
                     /locus_tag="PKM94_02395"
     CDS             49962..50666
                     /locus_tag="PKM94_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOD65516.1"
                     /translation="MVTVRRAEERGHFDHGWLDTYHTFSFDRYHDPNHMGFRSLRVIN
                     EDRIAPGTEFPMHPHRDMEIITYVLEGALSHEDSMGNGTVIRPGEVQKMTAGRGVFHS
                     ERNDLPNGRTHLLQIWIRPSENGLTPGYEQIRFDDEARRNRLLLIGTSDRSTDGKAVH
                     INQDARLYTCRLDAGNAVREPLAQGRYAWVQVIRGKLLVNGHELRTGDGAALSGEREV
                     AVEAAEGSEFLLFDLA"
     gene            50835..52493
                     /locus_tag="PKM94_02400"
     CDS             50835..52493
                     /locus_tag="PKM94_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOD65517.1"
                     /translation="MGHLKRYRQIAGALVRHGLHYLVGVFGLERFVPFHKGMLARGRP
                     SPGHTPAEHVRMALEDLGATFVKLGQILSTRPDLVPPEYLKELARLQDDAAPIPGDEA
                     EGIVAEELGAPVAELFLSFERAPLAAASIGQAHAAVLRSGEEVVVKVRRPGVVEQVTE
                     DIEIVRNLALVASRRLGPAEHYDLIGLAEEFAETLRGELDYLREGRNAERFAEHFRGD
                     PEVRIPRVYWEQSTASVLTLERIRGIKVDDTAALDGAGIDRRQLARRIARVLLKMILE
                     DGFFHADPHPGNFFVEPDGRLGVMDFGMVGVIDEAGRERLAMLLGALTSGDAERLVDT
                     VLDMSAGRGRVDREELKRDIQRLLTRYYELPLGQIPLGRMLEEALAAVRRHRLQLPSS
                     LALLFRALIVSEGLGLRLDPDFSLGPVLKPFAERLLLQRYSPLRWAQRMGRAMIDTDH
                     LLSELPRRLHRALKMLDRGGFEFGLRRESLEPMADRLERLANRIVLGMITAAFIIALS
                     VLMLVYPPGGGEGHWGGTVFAVGLVAAAGLGVYLAWRIIRTGRG"
     gene            52697..>53010
                     /gene="pth"
                     /locus_tag="PKM94_02405"
     CDS             52697..>53010
                     /gene="pth"
                     /locus_tag="PKM94_02405"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD65518.1"
                     /translation="MISLVVGLGNIGRRYAGTRHNLGFEVLDRAAAALGAPRATRTGL
                     DERTVCRRGDRTIVLAWPRTYMNRSGDAVAALLQDYGLRPAEVLVVVDDFNLPLGRVR
                     LRT"
BASE COUNT         9366 a        16708 c        17667 g         9269 t
ORIGIN      
        1 aggagcaccc cggcgaacac gaacacgacg ctcacgaaga acccgacgag gacgttctca
       61 ttgagctggc ggatccgccg ggaggcgaga ttgccggtga gcatgatggc gaagatgatg
      121 aggatcgcca ccgccccgac gtagatcagc acctgggccg ccgcgagaaa ctcggcctcg
      181 aggaggagga agatcccggc taccgagaac aggcacagga tgagaaagag ggcgctgtga
      241 aagatgttct tcagcgacac caccatgaag ccggacacca ggagcaccgt ggcgatgatc
      301 cagaaaaccg gcccgactcc ttcaaacaac tgttccatta ctccgcaccc tccgccggcc
      361 ggcccggatc ttccggggtc gtttcggtcg cggccttgcg ggccgccggc cgcgccggtt
      421 tgtcgtcctc gtcgccgccc gtgtcgcgcg tcgtgttggc gccttcggtc gcggccgccg
      481 ggccgatttt ccgcaccggg ttcttgcgcc gccgcgtgtc ctcgtagggc acgcccttgc
      541 cccactgctg gagcatggcg atgtcgaaca ccaggtccgc aggccgccgc cgcgagtact
      601 cgtagttcgg cgcgagcttg atcgcgcaga agttgcacgc ctcctcgcac atgccgcaga
      661 agcagcagag cgtgtggtcg acgacgaact gcgcgagctg cttcttcttg tcctcccccc
      721 gcggcgcatc gatcgtaatc gcccccgtcg gacacgcctt ctcgcacagc aggcaggcgg
      781 tgcagttgag ctcgccggtc tctttgtccg acagcaggac caccattccg cgcgaccgct
      841 ccgggatctc gtcccgctgc tcggggtact ggatggtcac ggcgtggcgc ccgaggtgct
      901 tgcccgcgat ctgcagaccc tggaggagcg ctttgaggtc gttgagaact cccataccgc
      961 gttgtccttg ccctaccagt accatccgag gtatttgaac acgccggccg cgatcagatt
     1021 ggcgaacgtc accggcacca gaaacttcca cgcaaactcc atgagctggt cgacgcgcag
     1081 ccgcggatag gtccaccgga acagcatcag cagcagcacc atgaccagcg tcttgcccag
     1141 aaaccagatc cacgacggca ccgccgccca gccgttccac ccaccgaaaa agagcgtcac
     1201 cgccaccgcc gacaccgtga acatgttcac aaactcggcc aggtagaaca tcgcgaactt
     1261 catccccgag tactcggtgc tgtacccggc gatgagctcc tgctcggcct cggggatgtc
     1321 gaacggcgtc cggttggcct ccgccgtcgc cgccgtgatg aaagtgagga aggcgatcgg
     1381 cccgtagggg aggcggaaga tgaaccagtt gaacaccagc cccgcctgcg acgtcacaat
     1441 atcgaccatc gagagcgagc cgacgaagat gatcacggtg agcaccgaca cgaccatcgg
     1501 cacctcgtag ctgaccacct gggcggccga gcgcatcccg ccgaggaggg cgtacttgtt
     1561 gttggacccc cacccggcca tcaggagcga gatcaccgtg aaggtcgtca ccgccatgat
     1621 gtagaggatg ccgatgttga ggtcggcgac gatgaggttg tctccccagg ggatcacgat
     1681 gtaggcgagg aaggcggcca cgaagccgac caccggcgcc cagaagaaca ccgcccggtc
     1741 gcgcgtcgcc acccggatat cctctttctg cagcagtttc agcgcatcca tgaccgtctg
     1801 gtaccagccg tgccagccgt ggcgcatcgg gccgaagcgc tgctgggtgt ggccggcgat
     1861 cttgcgctcc caccagacga ggaagagggc caccaggcag agcaccccga acatcatcgc
     1921 cccgagcgtc ccgtagcgga ccgggtcgat catccacccc gggaggccga ggtcgtgcag
     1981 gcgctcgccc agccagaaaa agacccggta cagctcgaac atctccagcg cgagcagggc
     2041 cggcagaatc atcggtcgac ctccggcatg atgacatcga gggtggcgaa aatcgccacc
     2101 aggtcggcga tgagcacgtt ggtggcgaaa tgcggcagcg ccatgagggc gttataggat
     2161 cccccgcgca gcttcacccg caccggcttc ttcccgccgt ccgcctgaat gtacgccccc
     2221 agctcgccgc gcgaattctc cacccggtgg tacacttcgc ccgccggcgg cttgtagtta
     2281 accgtgaccc ccttcgccat gatctccccc tccggcagtc cgtcgagcgc ctggttcacg
     2341 atccggatcg actgccggat ctcgtcgagc cgcagcatgt aacggtcgaa gcagtccccg
     2401 tttggccgcg tggcgacatt ccagtccagc cggtcgtata tgaggtaggg atcattccgg
     2461 cgaatatcgt agttcactcc cgaggcccgc aggttcggtc ccgaaactcc gtaggcgatt
     2521 gccaggtccg ccggcagcac cccgaccccc ttcgtgcggc gcagccagat cggattctcg
     2581 ttcagcagcc cggtgtagtc ggacatcttg gcgctgatta tcttcagcgc cttgcgcgtc
     2641 ccgtcggcaa aatccggcct gatgtcctgc gacaccccgc cgatccggat gtaattgtag
     2701 gtcagccgct gcccgcaggt ctcctcgaac agatcgataa tcagctcccg ttcgcgcagt
     2761 ccgtagagga agggggtgta ggccccgata tccatcgccg tcaccccgta aaacagcagg
     2821 tgcgaggcga tacgattgag ctcgagcatg atcgtccgaa ggtactgcgc ccggggcggg
     2881 acctcgatcc ccccgagttt ctcgaccgcc cccgcaaaca tgtacccgca ggacatggcc
     2941 gtgacgtact ccacccggtc catgagcggc acgcactggg cgaacgtccg gttctcgcat
     3001 atcttttcga tcgaccggtg caggtagccg atcaccggct cgaccttcat cactttctcg
     3061 ccgtccatcg tcaacagcag ccggcagacg ccgtgcgtcg agggatggac cgggcccatg
     3121 ttgacgatga actcttctgt ccgcagcgcg ttggccatcg ctcacttctc cggcatccgg
     3181 ataaagtccg gcgcatccca gtccttgcgc atcgggaatc cctggtccca gttgtctggc
     3241 agcagaaact ggcccagctt gccgtggttg cgcacatgaa tcccgtacag ctcccagaac
     3301 tcccgctcga accagtccgc cgcgggccag accgcctgca gcgagtccac ctccggcttc
     3361 tcggccggca gggtgatttt cagatcgagg cggcagctgt ggcggatcga ggccagcgcg
     3421 tagtggatct cgaagcgttt ctccggggcc gcggcggtgc cggtgtcgat ggccccggcg
     3481 ttgcacaggt agtccatccg cagctgctcg tcatcccgca gcgcccgcgc cacctcgagc
     3541 agatccgaag cccggttcac caggaacagc ggatcgtacc gcccggtcgc cagacgcgtg
     3601 acccggtcgc cgaacttacc ggccacgtag ttgccgagtt cttctctcgt cacgccggct
     3661 tctcctgcca atcgaggagc cgctgcttct tgattttctc ctggaggtgc aggcacccct
     3721 cgagcagcga ttccggccgc ggcgggcagc cggggatgta gatgtccacg gggatgatgt
     3781 ggtcgattcc tttttcgacc gcgtagcagt cgtagtagaa cggcccgccc ttgatcgtgc
     3841 acccgcccat ggcgatgacg tagcgcggct cggccatctg atcgtacagc cgccggagcc
     3901 gcggggccat tttcttggtg atggtgccgg cggcgatgag gaggtcggcc tggcgcggcg
     3961 tcgaccggaa gatcatcccc aggcggtcaa aatcgtagtg cgacgccccg gtgcacatga
     4021 gctcgatcgc gcagcaggcg gtcccgaaca gcaggtacca cagcgacttc gcctggctct
     4081 ggtgcagcac cgtctccagg ttcgtcagca ccagcgatcc gccggggatg tgctcaaggt
     4141 agacggggag tttccttatg acacccattt gagcgctccc ttccgccagg catagtagag
     4201 accgaaaaac aggatgagga taaacaccac catctccacc agtgcaaaca gcccgagttg
     4261 cccgaacgcc accgcccacg gaaagaggaa cactacttcc acatcgaaca gcacgaaaat
     4321 gagggcgaaa atatagaagc ggttattgaa ctgaatccag gccccgccga tcggttcctc
     4381 cgcgcactcg tagttcatgt ctttggccgg gtacgggtgg tgcgggcgaa cgagccgggc
     4441 cacgaagaag gtgatcagga caatggcgat cccggcggcg atgaacatca gaatggcgag
     4501 gtactccgac atattccttg acctttttta gctgccctct ccaacatttg cgtcagtcgg
     4561 cgcatgtgac tgaattcaca aaagttcgag agcgtaacaa atctcttttt ccccccgtgt
     4621 caagcgattt ttggggccat ttgcaccacc aaacctgttc cgccacaata ggaaacgaga
     4681 accgttcggc ggctctatag tgttcgccga aaccgactta gaaaagagga ctcgaacggt
     4741 cgattaaccc gaaaacgata cgattctgta taaaataacg aactctcatc cggattcgcc
     4801 cggccgcgcg ctcgctacct gagccgccgt atcttcagcc acagcgcgac cgccaacacc
     4861 gtaatgcaca gcgtcgccaa acccagtccc ttgcgccgga agtagtactc gtcgaccagc
     4921 cgttcgccgg ccccgatggt ggcgctgatg agggtgaacc cctcggcggc tttggcctcc
     4981 gcctgttcgg cgctgagcgc gtgcacggtt gtccgcagtt gaatgagcgc ctgccggata
     5041 tcctgcagcc cgaaggccga ttcggtcgtg gccatcccga gtccgcgcgc ccgggccacc
     5101 accgcctgag ccgagtcgta cccggtccgg agcgagtcga ggttgtgcga gatggccgcg
     5161 gccacggcaa aggcccgcgt cccgtcgtcg ccgctgtggc actggatgca caccgccggc
     5221 tcgtccgttc ccacccactc gtcggtcggg tgttcgatcc tgtggttgcc gtggcatgtt
     5281 tcgcacatgg ggaagccggc cgccgcaaac gcgggggcgt ggggcgactg gttgaacagc
     5341 tccatctgga aagcgtggca ggtgccgcac acggccgaca gactcgccac ccccggcggg
     5401 gccgccccgt ggttgccgtg gcagtcgttg cacgccggcg ccccgaggtc ccccttgtcc
     5461 agcagcgcct ggctgtggac cgaggccgtg tagtcggcga cctgatcggt ggcgatgccg
     5521 tactcggcca tgtaagccgc gtccgcgtgg cacttcccgc aggtcgccgg gatattctgc
     5581 gggtgcgtgc gcgagtgcgg catcgcggca tccccgatat cgtgcgcccc gtggcacgct
     5641 acgcagtcgg ccaccttgcc gtctttctgc gcgaacagcc gctggccgtg gaccgaggtc
     5701 cggtacttcg ccagctggtc agtcggcagc cccgggttgt gttcgtgcat gtaggccggg
     5761 tcggaatggc agcgcgcgca gaactcggga atctccaggt ggcccggcac tccccgccag
     5821 tttttggccc ggcgcacttc gtccatatcg tccagcgccg ggtcgccgcc gtggcagtcg
     5881 tggcacgaaa tcccgttctg cccgtgcacc gaccgcgcct cggcgaacga gggtccgtcc
     5941 tcggcctcga acgactggtg gcaggccacg caggcgctct cctgggccgc cgcgatcccc
     6001 ggaatgaccg tcgccccgag cccgatgagc aatgccagtg tcttcaccgc cgcctcctac
     6061 tccagcaggt atccgaggat ggtcatgaca atcatgaaag ccgccaccgc cgcaccgaag
     6121 gcgagcgctt tgggcgaccg cccgccgcgc gcggagggac ggtcgaggaa cgggatcagc
     6181 gcccagatca cgccgcccgc cgtgaacacg agaatgccga acagctcccc ctcgataaac
     6241 ccgatgtgcg ccgggagaaa cttcagcgcc tggaacgcga acatgaaata ccactccggc
     6301 ttgatcgctg ccggcgccgg ggccagcggg tccgccttct cccccagcgg ccagtggacg
     6361 accccgattc catccgggaa gagcactgcc agcaccgcca ggatattgag cacgatcagc
     6421 cagaccagca gatccttgag caggaaatgg gggaagaact tttcgtaccg gcgcgcctcg
     6481 gccggaagcg cctgccacgc gagcggctcg ctcatgccct ggcgctggat gaagatgagg
     6541 tgcgccgcga gcaccacggt cgtgatcccc gggagaatcg cgacgtggag gccgaagaac
     6601 cgcccgatgg tcgcccccgt tacctcctcg cccccgcgca tgatcgtcag cagcgggccg
     6661 ccgatcaacg gcaccgcccc cgccatcccc gtccccaccc gggtcgcaaa ataggccagc
     6721 tcgttccagg gcagcagata ccccgagaac ccgaatgcca aggccaggcc gagcagcgcg
     6781 atccccgaca gccaggtcag ctcccgcggc ttgcggtagg aagccgagaa gaacacgctg
     6841 aacatgtgaa tgaagaccgc gaggatcatc aggttcgccg accacgagtg gatcgaccgc
     6901 atcagccagc cgaacggcac ctccgccacg atgtactgca ccgactcgta ggccgagtct
     6961 gcgcccggcc ggtagtacat gagcagcagg aggccggtga cgacctgcac cgaaaacagg
     7021 aagagcgaga tcccgccgaa gtagtaccag aacgaggccc ggtgctgcgg cacctgcttg
     7081 tggcccatgt aggcgacgag gttctcgatc tcgtaccgct cgtcgagcca gcggtacacc
     7141 cgcccccccc gcgccttcgg cccggacccg ccccccatca cgcctcctcc ttgctcacga
     7201 tgatctcgcc gtcgatgatg ttcacccggt agggcgccag cggccgcggc ggcgggcccg
     7261 agatgtttcg gccgtcgtag ctgtacttcc cgttgtggca ggcgcaccac aggatcccct
     7321 cgtccggccg gttctgcacc gtgcagtcca ggtgcgtgca ggtcgccgcg cacgcccgca
     7381 cttctccctt gtcggtcacg aagacgatcc ccaggtcccg cccgaacttg aaatacttct
     7441 gcttctgctc cgccgcctcc agatccgcct gcgtgaactc cagctttttc agcgcgacat
     7501 ccgtctccgc caccgccggc ggcgtgatgt acttgatgat gggatagatg atcgccccga
     7561 gcgtggcgag ggcgctgccc ccgaggatca gattcagaaa accgcgacgg tccgtgtttt
     7621 tctcagccac gctgcacctc acccgactgt cggcccaccc gtaactcctc catctctgcg
     7681 cgccgcccat gaaccccgga cattcaccga acgccggcgg ccgcggccgc gcccgtctca
     7741 gcggagacga aaacgaaaga ggggcgaaat gtcaacaata aatatcgaaa cggtccctga
     7801 tcggcgccgc ggcggcgccc cctcacacca ccgcgatcgc gtcgatctcc accttcacat
     7861 ccttcggcag ccgcgccacc tggaccgtgg cccgggccgg cgggttggcc tcgaaatacg
     7921 atccgtagac ctcgttgacg gccgcgaaat cgttcatgtc ggtcaggtag atggtcgtct
     7981 tcaccaccgt ggagaaatcc acgcccgcgt cggccagcag gccgagaagg ttctccatca
     8041 cctgcttgca ctgctcggcc gcggtcttgc cgaccaccgt ccccgtctcg ggatcgagcg
     8101 gaatctgccc tgagcagaat atcatcgtgc cggctgccac cttgatcgcc ggcgagtacg
     8161 gcccgatcgc tttcggcgcc cggctgctct tgatcgcttt gcgcatatct cctccgtaag
     8221 agtcttcgca ccctataccg tcgttttcgc cgcccgaata taccgcggtc gcgggcccgg
     8281 cccaagcggt tattcggcga ggggtggtgg agtggattcg gccttgccga ttccggcgcg
     8341 cgtgcctata ttacaggcgt ccacttgaaa accattgcga gggcgggtcg ttgccgcgag
     8401 cggcagtccc cggagtccag ttgccggggg aacgctgcag ggcgcgcgcc cctgccctca
     8461 ggagagagta atgaagcgcc tggccaccct gctggttctt tgcctggctg cggctatggt
     8521 cgccgccccg gcggctgccg cggccctcga ccgggcatac gcccgccacg ccgacacctg
     8581 gcttgacttc cccgctttct atgccggctg ggactccgac gacagcgccc gctgcctggg
     8641 cgcctcgcac gcgctccacc cggcggtggt cttctcgccc cgcggcttcc tcggctaccg
     8701 cttctggatg gcctacacgc ccatctgcat ctccgagtcg gacgagaacc cgcaccttgc
     8761 cgtatcgaat gacggccggg agtgggcgga attcaccgcc gcctccggct gtctggccaa
     8821 ccccgtgttc gaccgatccc tcttccgcgc tatccacctc tccgacgccg acctgctgct
     8881 cgagggtgat tccatcctgt acctcctctt tcgcgccacc tgggaaatcg ccggcgtcga
     8941 cagcaacgcc ctgtacgcaa gcgcgacggt cgacggtctg gtgtggacgc cgccggtcaa
     9001 gatcctctcc gacggcctcc tcggacagcc ccggcagtcg gccttcgtct cccccgcgct
     9061 cgcctccgag ggggactcgg ttttcatcct ctgggtggtg gagccccgcg ccgatggagt
     9121 ctcttctctg gacacctcgc gcatggttga gtaccgcgcc ccccacccgt tcggtccctg
     9181 ggcgctcgtc gacacgtgcg cggtcgctct gccctccgac tcgctgaaaa tctggcacct
     9241 ggaggttctc gcggacaccc ttttcggccg caccgccctg ctcaacctct cgcccaacag
     9301 cggcctcaac ggcggcgact ccgccgatct atatatggcg gcctacgatg aggcggccgc
     9361 cctgtggacc gtcgccgccg aacccgtgct tacctggcgg agcgacacct cggcctggta
     9421 cggtcgccgc atctaccggg cgagcggggt ctggatgacc actcccgccg acaccatcct
     9481 ctggctctac tacagcgcct acgcccgccg cgccaacgcc ggcggggccg ggaccgggtg
     9541 gcagaccggc ctgacctggg ccgcctttga ctccgcgctc ggccccttct gcctggcccc
     9601 gtcgctgcgc cacgaaccga acctggagca cgtcgtcggc ctctccccca cgttccaatg
     9661 gctctccgcc gatcccgccc tgcaccgccc gcaggacgag gtcagcctcg agttgatcga
     9721 ttgcaccggc ggtgacagcg ttgccgtctg gtcggtctgc gtgtccggcg gcgggggcgc
     9781 cttttgcccc gattctttcg aactctcacc gggccacacc tgcgtcctcc gcctgcggag
     9841 ccgccacggc gccgcctggt ccccatggac cgcccttgag ttccgcacca atgccgcccc
     9901 ctccccgccg gcgtgtctct atcctcccga tcaacccgcg ctccctctct acgacctgac
     9961 ccccgctttc ttctggtcgg aggcgatcga tcccgatccc ggcgatcctg tgcgctaccg
    10021 tttcgccctc tgcatcgccg acacctgcgc cgactcgacc gacatagtga ccgacggcat
    10081 ttcgctgacg ctcgccgact ccctttcggt cgccacggcg taccgctgga cggtcgcctc
    10141 ctgcgacacc ttcggcgcct gctgcccggc gggtccgccc gcgcagttcc ggacctttct
    10201 ccccggcgac atgaccgccg atttcaggct cggcatcgcc gatctcaccg gcctggtcga
    10261 catcctcttc cggggcggca gcctgtcggt tccgatgatc ggggcggaga tggatggccg
    10321 ctgcgggctg accgtggccg acctgaccgt cctcatggcc cacctcttcc gaggcggtgc
    10381 tccccctccc cccggctgcc gctgacccgc cggcagtcgg cggtctcggt tcggcagccg
    10441 aaaacccgtt gccccgcgga cggcggcccg ctatcattcc cctggaatca gattgaccgc
    10501 cgacccctaa cggcaggagg gattatgccc acctttatca tggccatgag tattgtgccc
    10561 tcggccaaga agacccaccc ggagctctcg catgatatca atcgctccct cgaagtcttc
    10621 cacgccaaca aagtgaaagt gcacagcctc tacgcgaccc tcggccgcta cgactacatc
    10681 gccgttttcg actgcgatga ccagacgtgc gccttccgcg tcgccaccgg catcaacgat
    10741 ctcggcgttc tcgataccga gacctggccg gtcatcccgt acgaggagtt cacgcagttg
    10801 ctgcagtaga cgccgccggg ggcggcgtct ccgggacacg cggccggcgc gggcccgccg
    10861 cggaagacgt acgcgatgag gaacgtgatg tcggcaaccg ccaacttccc gtctgctgtt
    10921 acatccgcct cctcaaagca gtcaggctga ggcccgccgc ggaaaacatg gttgatcaga
    10981 aacgtgatat ccgccaccgt taccgctccc gccgcatcga agttgacatt ccccctggtt
    11041 ccattgcagc atttggtcca ggaagccaaa taccccgaca ccttgccgcc cgccgtggtg
    11101 aatggtcccc ccgcgatgag gttgccgtcg tacacggtca gggcacggat cgtgccatct
    11161 gttccagatc ccagcggcct ccaggtggag ccgttccaag aagcgatgtt ggaggcgatc
    11221 acatcgcctg cgacccgaaa ggaccctccg acaacgagat tgccgtcgta gacgatagcc
    11281 acgtacacgg ggccggcgac gccgggaacg gatggggaga tgctgtcgtc ccaataaacg
    11341 tcgtcaggat catcctgtgg tgatccctgc agcttggctg taagaaccgg ctcaccggtc
    11401 acggggtcac gatcgatgtc aaaaccgtcc agatcaagac ttccctcata accggagcga
    11461 cgcacctcct ccagatcgaa gcggccatcg ggtgtcagga agcgcccgac gctctcccct
    11521 ttattcggaa cctcaggagg cgtaatcccg tcgtcaagct tcggcagttc gagcggcaat
    11581 ttcccggccg gcgcagtctc taccggttca ccggccaggg catttaaccg aacggcattc
    11641 gaaggctggc cttgacatgc gattgaaaca acgggtatcg acggcagcaa ctgcgaagtc
    11701 gatgccggct gccgcacatc accctccgca gacaacgtcg cgctgctcag aagcgcagcg
    11761 gcaacacaca ccataatgat agctcgcatg atactctccc taagactaat ggcgccaatt
    11821 acgccgccaa tcctcaggca acgaatttgg gcctgctgcg taggcataat atgcacccga
    11881 ctccccaatc ggacaggaac attcggccgg cgcggccggg cgtcggaacc gaagggatag
    11941 ccctccggcc atggggcctg attttctgcg caaggcagcg gctcgtgata gactatcgca
    12001 gtatcttcga atcgactccg cgccccggtt tcagcgctga caccgtccgc aatcaaccgc
    12061 attaggagaa ttgacaggtt gcggaccaag cgtacgatgg agcccccggc cgggccggtt
    12121 caacacgctg tttgggcaga gcttctgggc gcggggcaca gccctcattc ggacaggtga
    12181 ccgggagggg cacgggttcg accgtaactg gctagcgagc actttcgagg accgaaggcg
    12241 gggcagggcg accggccggc tgagttgacc gtaagattcg gttttccggg ttggggaatt
    12301 cgggccccga tggcccccta gcggcacgcc cggcccccgg tccgagagcc gtcccggcgg
    12361 accttcccct cggcaaggaa ggcgctagtc cgccagaccc gtcggcccca tcagttccac
    12421 gaacgccttc aggaaatcgt cgcggtacgc ggtctggaga gagaacatga ttttcagcgc
    12481 cggaaaagtg tccattgctt tctggtacac gcgctcggtc gtcatggcgt cgaacgagtc
    12541 cgcaatggcg acaatctgcg agtagacgtg catttcatcg agccgcagtc cgttgggata
    12601 cccccgccgg tccccgcgct cgtggtgctg gagcaccggg tagtagcagg cggcggcgat
    12661 ctggtcggtc tcttcgagaa tctccgcccc ccactgcgga tggcgcttca tgatctcgaa
    12721 ctcggtcggg ttgagggggg cccgcttgtt gagaatccgc tccgacaccc gcgatttccc
    12781 cacgtcgtgc agcagcgccc cgacgccgag ctcgttgaga aacgtctcat ctccgtaccc
    12841 cagctgctgc gccagggcca gcgagaacgt gcagacattg accgaatggg tgtaggtgta
    12901 gtagtcgaag gaggtgatct tgagcagggt gtgaaaggcc tctttcccct gcaggatgaa
    12961 ctcgacggtg tttgtcacca ggtcctttgt ccgctggatg ttctcgccgt aggtggggtt
    13021 cgccagcact tctttgacga ggttggtcga ggtgtcgtag aggatgttcg ctttcttctc
    13081 ctcccggatc ccgggatcgg tcatgatctt ggggaggttg cgctcgatgt agcgctggta
    13141 gcgggaccgg ttgtcgtcgg tgatgaagag ccggtcgacc cggttgtcca ggagggtgcg
    13201 gcgggtgcgg tcggtgaagg ggaggtcggc cgaccggtac agcaccatgt tgccgttgac
    13261 tgtcaggtac aggtcaaagt cgaggacgga gtcgacccgc agcgagtcca ggaggatcgg
    13321 caggtactgc ggcgtgacgg taattttggc ggtgccggaa gccataatcg gagagttctc
    13381 cttgtcgccc gggcgcggct gcccgatttc gggcggccgc gcgttcggtt agtttatcgg
    13441 cacccggccg gagaggattt aaaaaaaccc tccggccggg cggccggagg gcaccgtagg
    13501 agggatgcat gagaaaacat ggttcgcggg tcacgaacca taattcttga ttgtcccgag
    13561 caccgaattc agcgaggtcg tgatgtcgct caggatctgg ctgtaccgct tgagcagctc
    13621 cgaatactgc caccgcatct ggaaggcctt ctcggggaaa tcgaacagct cgagcagagc
    13681 cgccttgtga atctcgtcga gatagcggat cgtgtagtag ggatagtccg ggatattcgg
    13741 atcctccacc tcgccccggc tgagccgctc ccggtagacc tcgagcttct ccagcaccac
    13801 ggtcccgaag caccaggggc tcatgacgac gccgaagttg ccgccgtccg tatagaaccg
    13861 gatgattgtc tgcatcagga ggtcgacaat cacgccgcgc gcccggtagt aggcgatcag
    13921 ctcgctctgc tccggcgccg tcgtgaacag tcgctcatcc agccgcgacc gctccgccct
    13981 ggccttggcg tatgtccgtt cgaggaaccg atagaaatcc tcgaaataca ccttggtgtt
    14041 ctccgacagc gacatcagga aatatccccg gctgtctttg cggacccggg gttcctgaat
    14101 ggcggccagg cgctcctgcg agaatcctcg gcgatatcgg ttgtccaaca tgctgtctcc
    14161 ttcggtaacc tcgtctggga gtgggtagag cgaagtttgt gccgtccccg cggcgaaccc
    14221 tcaggcccct gtctcggcat gttatttcgt tgtcaatcag cagattagtc ggcaattggc
    14281 gcggtggcgg tgtcaccggg gtggttgtgc agttgcttac atctgcttgc acccatgacg
    14341 attacggtca ttcgtatgaa aatcgctgca tagtcatccc cacccggatc ctggtcacgg
    14401 acgcgggcgg ggataaaagt ccggtccccc ctcgccgata tctaggctga aggtgcacat
    14461 ctgcgattct cttagttctc tggaggcagc atgcgcgacg aggcgcagcg cggaaaacac
    14521 acatcttccc gcatgaaacg taaaggtcgg ccggggcagc gccccgcacg gcatttccgc
    14581 ggcggatccg atgaagccgg ccggcgccct gaccggcgcc gccaactcga acaactggcg
    14641 gaaaccgaag cggaggaaat gctccggcag gacgagtccg acgagttttc cccgtcgtga
    14701 ttcactctcc tgaccaccga tccgacaaac gcagacggtc tcgtctgcgt ttttttgtag
    14761 cgcgcggatc cggccgctgc ggggagcggc ggaaacggcg aaatcgcttg acagccaagc
    14821 aggtccggcg taagttccgc aatctgaagg aacgtgtgat aacctcgtaa gggaggacta
    14881 acgacatgaa gtgccgctgt ttgccagacc tgcattgcgc ctctgcggcg gcgattactc
    14941 ttgcgttctt ggctctggtg attgccggtt gcagcgacga cgagaccact ggaccgggcg
    15001 gcggcggcgg cggcgcctgg acgctcgtgt cgccgaccac cgaaggaaat ctgtatcggg
    15061 gcgcctgctt cgtcgatgcc accaccggct ggctggtcgg ggtggacggc cgcattgtga
    15121 agacgaccga cggcggcacg acctggattg agcaggccag tcagaccaac cggacgcttc
    15181 gcgctgtgga cttcgtcagt cgccaggacg gctgggtggt cggtgatgac ggccgcgtgc
    15241 gccgtaccga agacggcggg accacctgga gcctgccgct gctggcggta accgatcgct
    15301 ctctgtccgc ggtcgagggc gtcaccgcgc ggcgggtttt cgcggtcggc gaggaaggca
    15361 ttctctacta ctcgctggac gccgggcggc gcttttcggc ggtcccgctg gaagacgccg
    15421 ggccccttcg cgacatcgtg gtcttcgata cgagcgcggt agcggtgggc gacgacggat
    15481 tcatcgcccg gttcttcaac tacgattggg aaatcgaacg cctcacggcc gacacgacgg
    15541 tcgacacgct cccggtggat ccggacactc tgcctgatga ctttcccgcc gacaccgtga
    15601 tcgacaccac gttcgtgccg ggagatacga ttgttgacac gacttactgg tggccggaca
    15661 cgctcgtgga caccacctac tttacggtcg acacgatctg gtccggattc gagacggtca
    15721 cggcggaggt cgacgacaac cttctcgggg tcgcctatgc cggcggcaat tctctctggg
    15781 cggtcggtga gaacggcgtc tttctgagtt ccgacgaccg gggcgctagc tgggaggtcg
    15841 tagacctggg cgtgaccgcg cacctcacgt cggttgccag cgacggcgcc ggcggcggaa
    15901 tcatcaccgg tctcaacggc accgtcctgg tcacgagcga caacggcgcg acatgggcgg
    15961 cggcgcaatc ggggtatgcc ctcgatctct atgcttcgtc gtctccggcg gccgacaact
    16021 tctgggcctc cggcgatgcc gccgtctttc ggtccgctga tgccggggag acctgggagc
    16081 agcgcgtgac cgggacgagc ttgctgccga cgctgacgga tattgttttt gtcagccccg
    16141 actccggttt tgccgtgggc tataccggca ccattctgcg gacgaccgat gccggacaga
    16201 cgtgggtaac ggtgcggcag gggtcggcct ttgccccggc cgagtggttc tcagcggcgg
    16261 cgttcaccga caatctgcac gtgtgggcgg tcgggcgcac ggggctgccc ccttactact
    16321 tgttggtggt gcggacgagc gacggcgggg aatcgtggga gcgctttgag ttccccgaca
    16381 acccgcccga cccggacaac ccggcgccgg acacggcctg gggcaagaac gtcgtcttcc
    16441 tcgatgatct gaccggctac atctgcgccg gcgacagcat cctctacacg accacgggag
    16501 gcgcgagctg ggtcaagcag gccagccccg acaccgtgac cccgccgcga accatccacg
    16561 acctgagttt cgcggacgcg acccacgcct gggcggtcgg caagaacggc ctgattctgc
    16621 agacgaccga cgggctgacc tgggagattc cgacggatac ggtcaagacc accctgtggg
    16681 ccgtcgactg ctttgacgcc aacggcgtgg tggcggtcgg caacggcggc gagatctggc
    16741 ggaccaccga cggcgggacc acctggagca aggtttcatc gccgacgaaa agcacgttga
    16801 tcgacgtgag ttttgtcgac gcccagtcgg gcaaggcggt cgggcagaac ggaaccgttc
    16861 tgagcaccgg cgacggcggc cgcacatgga ccgtccaggc ttcccccacc gccgagacgc
    16921 tcatcggggt ggagatgatc gatcgcaatg tcggctggat tgtcggcgga cacgctcccg
    16981 atgcctccgg ggtgatgctg cgcacgacga ccggcggcaa ctgattcccg accgtccggc
    17041 cgcttgcggc ggccggcgca cggagtgaaa tgaccaaggc gggcccgatg gcccgccttc
    17101 tcttttgaat atgtgcgcct ccgccgggcg cgttcaatcc ttccagtcgg cgatgaagac
    17161 gttggtctcg cccgggaccg cgttgtcgcg gttcgaacag aacaccagcg tccggccgtc
    17221 ccgcgagaac atcggaaaac cgtcgaatgt ctcgttgtag gtgatccgtt cgacagtctt
    17281 ggtggcgacc tcgaccatga acaggtcgaa gttgcgcccg ccggggtcgg ccagattcga
    17341 gcagaagatg acgcggcggc cgtcggggtg gaaatacggc gcgaagttgg cggcgccgtt
    17401 gtcggtcacc tggatgggtg tgcggtcggc cagattcatc acatagatct caagcttgcc
    17461 gggccggatg agtccctggg cgaggagccg ctgatagtcg gccagggcct cgccctccgg
    17521 gcgcgaggcc cgccacacga tccactcccc gtcggccgag aaaaacccgc cgccgtcata
    17581 ccccggcgcg tgcgtgagct gttcgacacg gccgccgtcg ggatccatca tgtacagctc
    17641 gaggtcgccc gagcgcaccg aggtgaacag gatcttgtcc cccttcggcg aatagacgca
    17701 ctcggcgtcg taccccggcg tgtcggtcag ccgcaccggg tcggaaccgt cggcgttagc
    17761 ccggaaaatg tcgtaggtct catggagcgc ccagacgtac ccctgcgaca tgtccggttt
    17821 gggcggacag gtgtcgcccc cgaggtgggt cgaggcgtag atgatgtggc ggccgtccgg
    17881 cgagaggaag gagcaggtcg tggccccctt ccccgtcgac accatccgca cctcggaacc
    17941 gtcaacattc atcgtgaaaa tctgatcgca cgacatggtg tcccgcatcg actggaagca
    18001 gagcctggtg gcgtcggcgt tgaaataggc ttcggcgttc tggccgccga aagtgagctg
    18061 gcggatgcgg tcgagatgcc gctcgccccg gaagtgggtg gcgtaggtga gcgtgtcggc
    18121 cgccgttttc ggagcggcgc tccggtccac ctctttcaca caccccgccg ccaaaacgag
    18181 agccgcgagc actgcgaaga ttcctttcac ccggtcctcc atcaaatgct gttgccaatg
    18241 aagaggttat gctgcatata tgtctaagaa aaatcaaccg ggagccaaag tacaatgaga
    18301 attacgctct ttgtcatggt cctgtcactg gcgatcggaa ccgccgcggg ggagtccgaa
    18361 accgacgtcc tcggccgaat cctcggcgaa gtgggattca cgcgcgcgga tctcggctac
    18421 caccccgcgg gctactggaa ccgcttcccg cttgacgttc cctaccggct gacctcgttc
    18481 gatgcgctct ttgccgaacc gctgaagctg tacgactacg ccacaaccat ggggaacgcc
    18541 gtcgagctgt accttgatcc aacctacgcc gacacggctt ccgacggtct ctaccagttg
    18601 gtgtacaacc tgggggtcga cagaaaactg gggggcttcc gctcctactc ggcgaacctg
    18661 cggccggccc cggcggggga agagccgctg gtcgacgcgg tggaggggct ctacgcgctg
    18721 gccggccgcg acaccgagtt ctacaccttc ggcaaccgcg ccgaatcgcc cgacatgacc
    18781 gcggagatcc gacagaaaac cgcgcgtctc ccggactcgg tgcaggttgt cctcgcgggg
    18841 ctgattgtga atctcacgga tgcgattcgc tggcgcgcga cggcgctgcg caactgcgac
    18901 gttgcggacc tcgaggctgc gctggcgatc cgcgacctgg cgagcacgca gggggacggg
    18961 atggtgtact acccggagct ggacgatatc gccgccgcga tcgatcttcc ctcgatgcac
    19021 tacgccgcct tgaagacggc ggccgcagcc gagagcgccg agcgcgccct gcgccgattc
    19081 gcccgcgcgg tccccgacga tttcaccgcg gaactcccca cgcctttcgg gacgatcgcc
    19141 ctgttctctt cggtcgcccg gtccgagcgg atggccccgc cccgcctctt cggcgaactg
    19201 cgcgcctcgc tcccgcagtg gacggagtat gatgcgacca acacgctcct cgtcgtcgat
    19261 ttcggccgcc ggatgatcta ccaggggacg ccgggcgcgg cggcgtcgct gggaaacccc
    19321 gtgtcggtgg tgatcgatct cggcggcgac gattactacg gccgcgagcg cgcccagtac
    19381 ccgcctgcgg ccggtgtcgg gctgtgcggg gtcggcctgg tgatcgacag cgagggggac
    19441 gaccgctacg aaggctccac ctatgcccag ggggccgggc tgttcggagt gggcgtgctg
    19501 ctcgaccgcg cggggaatga cgagtaccgc gccgaactgt cgagccaggg agccgggtat
    19561 ttcgggatcg ggctctgcct cgacggcgtc ggcgacgaca cctactacct gtgcggcgac
    19621 ggccagggga tgggcggggt tggcggaggg gtgggcgtgt gcgcctcctt cggcggcgac
    19681 gaccgctaca ccgccgaacc gctggctgag aaatacaacc gcggcgacta ccactcccag
    19741 aacaagatca acggcaacaa cgcccagggg gccgggttcg gacggcgcgg cgacggttcc
    19801 gacggccatg cgtgggccgg cggcctcggc gcgctcatcg atatcaacgg cgacgaccgc
    19861 tactactccg gcaactggac gctcgggatc ggctactggt tcgccaccgg gatcgcgtac
    19921 gacaaacgcg gcgacgacct ctacgaatcc tgctacttca cccagggttc gggagcccac
    19981 tactgcaacg gcatcctcat cgatgaaggc gggaacgacc ggcacgaact gtacgagacg
    20041 gccggggcgg ggctcggatt cggctgggat ttcaccaacg ccctcctcgt caacaagggc
    20101 ggcaacgacg tctaccgggc gaagatgatc tcgatggggc tggcccagat ccgctccaac
    20161 gccttcctca tcgacattgg cggcgacgat gcgtactatc taggacaagg gacgcccggt
    20221 ctgggcgaag ccacgttccg cgaggggtac gaccggcccg gccggctgac gacctactac
    20281 gcctacgcct cgtccttcgg cggtttcatc gacatcggcg gccgcgaccg gtacgtgtcg
    20341 tttacggaca gcggggagac gacacatccg tgggcggccg acgatacgct ctggtttcgg
    20401 ccggcccgca cggattcgct cttcggacac aacaactacg gcgttggagt ggacgcagaa
    20461 gccggggcgg ttccggaact ggagaagtgg aagtgaatga catccggcct aatcacggcg
    20521 ggagtggaca tccaaatcaa tagacacccc gggcaggcac ccggccaggg tcggtttcct
    20581 gccccgcgag tgtattttcg cgagcgcgcc ggatttcgcc ttccggcgag agggagggat
    20641 cggggaggat cccgatcggg ccgtgtcggt ttgcccatac cttgtgcttc atgtcatcgg
    20701 tgaacagcta tcggcagcgg ctttcccggc gctctcccgt agctcggtcc cgtccccgat
    20761 ggccgatccg gacaggccga acatgaatct ttctcggtgg tgatcagacc gaggccgaag
    20821 ctggacccgc ccatctcaag attggcaaat cgcagcacac gtcccttaga ggcgggattg
    20881 gccgccggct caactcaccc gcccggctcc acgccggaga gtaccttgcg cagatagggc
    20941 agccggtcgc gctcgccgat gcggccgaga tcgagcatga tctgcacccg tccgatcatg
    21001 gaaaactcca tcgggcaatc ggccccctgg cggagcgcct caaccgtccg gcgcgtcagt
    21061 tgtcggccct cctcgaggaa cagggagacg cggccggcgg ccattttcac tttctcgacc
    21121 ttcatcgcgc cggcgaggat cttcacggcg gtggcgtcga acaggttcac cgccgactgg
    21181 ggctgggcgc cgaaccggtc ggtcacctcg tcgcggatcc gctccacatc gtccagcgtc
    21241 cggccgtcgg cgaggcggcg atagatatcg accttctgct ggcggtcgtt gatgtactca
    21301 tcggggaggt ggagttcgag gtcgagttcg agcttggtgt cggggggacg gacgagttct
    21361 tcgcctttga gctcggcgat ggcctcttcg agcagcttat tgtacatgtc gaagccgatc
    21421 tcctcgatgt acccggattg cctcggcccg agcagggttc cggcgccgcg gatttcgaga
    21481 tcgcgcatgg ccagggcgaa cccggcgccg aggtcggagt gggcctcgag cgcgcgcagg
    21541 cgcttgatcg cctccggctg gagctgcttg accggcgggg tcatcaggta ggcgtaggcg
    21601 cgggtggcgc tccgcccgac ccgcccgcgc atctggtaga gctgggccag cccgaaccgg
    21661 tcggcgcggt tgatgatgat cgtgttggcg ttcgggatat cgaggccgga ttcgatgatc
    21721 gaggtgcaca ggagcacgtt gtagcgtcgg gcaaggaagg cgagcatgat cccctcgagc
    21781 gaccgctcgt gcatctggcc gtgggcgatg gcgatttcgg cctgcgggac aatcttcttg
    21841 aggtagtggt gcatcgcctc gattgtttgc acccggttgt ggacgaagaa cacctggccg
    21901 ccgcggccga tctcccgcag gatggcggtg gcgatgatcg ccgggtcgaa ttcgacgatc
    21961 tcggtgatga tggggaggcg gtccttgggc gaggtgttga taatgctcat gtcgcggacg
    22021 ctcatgagcg acatgtggag cgtgcgcgga atcggcgtcg ccgacatcgc cagcgtgtcg
    22081 accgccgctt tcaggtgccg gagtttctcc ttgtggcgga cgccgaagcg gtgctcttcg
    22141 tcgacaatga gcagaccgat gttggggatg aagatatcgc tgctgagcag ccgatgggta
    22201 ccgatcacga gatcgacccg gcccgcggcg agatcctcca ccaccgcgcg ctgctgcttg
    22261 gccgtccgga accgcgagag catctccacg cgcatcggga attcggcgag ccgttcggtg
    22321 aacgtctgga agtgctgctg ggcgagaatg gtggtcggca ccagcaccgc gacctgcttg
    22381 ccgcgctcga tcgccttgaa cgccgcccgc accgccacct cggtcttgcc gtagccgaca
    22441 tcgcccacaa tcaaccggtc catcggctgt tcgcgggcca tgtctctctt gacgtctgca
    22501 atcgccttga gctggtcggg agtctcctca taccggaacg aggtctccag ctggcgcagc
    22561 caggtggtat cctcgccgaa ggagaaaccg ggcgcgctcc ggcgccgggc gtacagtttc
    22621 aggagatcgg cggccatgtc ggcgatcgcc ttcttggtcc gcaccttgag cttgtcccac
    22681 tgggggccgc ccaggcgggt cagctgcggc gcggtgtctt ttccggcgta tttggagacg
    22741 cgattgaact cttcgatcgg gacatagagc ttgtcgttgt cttcgtaaag gagggcgagg
    22801 cagtcgcggt tgcggccgtc gaccacgagc gtctccagcc cgaggtattt cgcgatgccg
    22861 tgatcggtgt ggacgacgaa atctccgcgg ttgagcgcag agtagtcgga gatcgcgacg
    22921 ccctcgcgga acttcttgcg ccgcacgcgg cggtggtggc ggccgaatat ctcgtggtcg
    22981 gtgagcacgg cgaaacgccc ggcgggacag acaaaccctc ctttgaggtc ggccggctcg
    23041 atcaccggcc ggctctccgg gccgagccgc tcgccgatca gctcatcgag ccgctcggcc
    23101 tggctctcgg tgtcggtggc gacgaagtgg accatcccga gcgcgtcaaa ctcgcgcgcc
    23161 gtctcggcca gcaggtcaag gcgcgagccc agcgaggggt gcggcctgca accgaagtcg
    23221 atgacgtcgc ggcggccgcc gcggaaaggg gcgtggtcga tgcgggggtg ggcggccagc
    23281 caggcaaagg cgccctcgac cgagtggtag taggcctcgg gcgcgggaag cttctccagc
    23341 cgccccttga gccgctggta gagaccccgc ccctcggcga ggatcccctc cgcctcgtcg
    23401 cggagcaagt gctccccttc gagccagacg acggtgtccg gcggcaggta ctcgcggagc
    23461 gacccggggc ggacaccgaa caggatcgcc agccactcga gccccggcag ctcggggtcg
    23521 ttgaggtagc ggtcgcggat gtagtcggca tcgtgctccg gcagcgccgc gaggtattgc
    23581 tcgactgtct ccatggtgat cggcacctcc cggcgcggga ggaggttgac ccggtcgacg
    23641 cggccgatgg tgcgctggga gccgacgtcg aagagacgga tggtgtcgac gtcgtcgccg
    23701 aagaattcga cgcgcgaggg agcggtcgag ccgggggaga agaagtcgac cagcccgccg
    23761 cggagggcga agtcgccgac ttcctcgacc accgggacgc gccggaaacc gagcgcgacc
    23821 aggcggcgca cgaggtcatc gaggtcggcc gcccgtccga cttcgagccg gacccggctt
    23881 ttttcgagat ggtcgcgggc gatcgtcggt tcgagcacgg cccgcagcgg ggcgaccacg
    23941 gccgccagcc ggttgtccat gaggtcggcc agcgacgaaa tccgcctccc catgacctcc
    24001 ccctgcggcg ccttgaagtc gtacgggagg acctggcggg cggggtagtg gccgacgcgg
    24061 tccgggccga gcagcaccgc cagatcgtcc gccagatcgg ccgcctgctc ggaggtgacg
    24121 gtcaccgcca gcaccggtcg gccgagcgta tcggtcaggg cgcgaaggag gaaagcgggc
    24181 gccgagccgg ccagcccggc gatcggaacc ggttcggccg ggccctcttc aaggcgggcg
    24241 gccagttccc gcgccggggc gtattcgagc caccggccga tgacctgccg gaggcggctg
    24301 tgggtggtgt gcgaacgatc cgtgccggtg gtcatgcgct gtagctgctt tcgttcccgc
    24361 gggcggtcag gcccgcgcca aacacaaaaa cccctggccg tcgccgaccg gggagggtgg
    24421 ttcactcgtc gctggtatta tacaccgccg gcggggcgga tgccagtttt ttgcgcgctc
    24481 actcctgcgc ttcggccagc gccatcaggt cggcgcccac ttccatctga tcgtacgcca
    24541 ttgaggcgtg ttcatagagc acgacgatcg tcacgcggcg gttgcgggcg tcggccgggt
    24601 tgtcggcgat catcgggaac tgctcggcga acccgcgcac ctcgcgcacc tggccgtccc
    24661 acagcccgtg cgcttccagc agttcgcggg cggcgttggc ccggtcggcc gacagctccc
    24721 agttggtata gaggaggtcg ccggtgtagg cggcgtcggt gtgcccttcg agcacaatcc
    24781 ggttctgaag gtgcccgagc tccccggcga tcgtcatgag aatcaccgcg ctctggcgca
    24841 ggagtttggc cgagccgggt tcgaagaacg ccggagactc ggccgactca ttgaggatga
    24901 tccgcagccc ctcggaggtc aactgcatct tgacgttgtc gcggaggcgg tggaaagcct
    24961 cctgctgctc aagctggctg atgatcttga gggcggcctc tttcatctgc ttcttctcca
    25021 tttcggaggg gccgccgccg gatttgtcgc tttcgttctt gagcatcccg gagggggtat
    25081 ctttctcggt gatggcggag cgcgagcctt tcaggacgcc ggcgcccccc tgctcgaggt
    25141 atttgccggg atcgcggaag tagccgccga ccgcttcccg cacctcgggt ttctgcccga
    25201 ccagccacat gaccaggaag aaggccatca tggcggtcat gaagtcggcg aaggcgactt
    25261 tccaggcccc gccgtggtgc ccgccgtgca cgtgcttctt gcgccgtatg atgatcggtt
    25321 gttcgtcggc catggctgcc gtcaccgctt cttggtctcg ttacaggctt tctcgagttc
    25381 gagaaacgcg ggccggacct cggcgtagag ggagcggcgg gcgaactcga cggcgatgac
    25441 gccggcaacg cccttgtgaa aggacaggag cgcgtgcttc atgcaaccga gatactggcg
    25501 catgtcgttg gtccggtgtt tgagactcga agagagaggt ccgacgaaac cgtaagcgct
    25561 caggatgccg aggaaggtgc cgacgagcgc ggcggcgacc ttgtgcccga tgacctccgg
    25621 cggaccgtcg atcgcggcca tggtgatcac cacgccgagc acggcggcga caataccgag
    25681 accggggaga gcgtcggcaa tcgcacccag ggcggtcgac ggccgcagct cctcttcgtg
    25741 gagggtctcg atgtcggcgt ccatgaggtc ttcgagatcg tgcggttgga cggctccgga
    25801 gatgatcacg cgcatggtgt cggtgaagaa cgcgaccgca tggtggttct tcagaaaccc
    25861 gggatagcgc ttgaatatct cgctctcctc cggatgctcg acgtggctct ccaggccgat
    25921 gaggccgtct ttgcgcgcca cgttgaagat ctcgaacatc atgaccagca ggctgaggta
    25981 atctttcttc ttgaggccgc ccccgagcgc ctgcttgagc tgcgcgaaga tgcccctgac
    26041 cacgggaagc ggggtggaaa tcagcagcga gccgagggcg gcgccgccga tgatgaggag
    26101 ttcggagggc tggttgaggg cgaggatttt gcccccgtgg atggtgtaac cgccgaggac
    26161 accgccgagg acgatcaggg caccaatgaa tatgaacata gcgcgttgtc cctgtgtaag
    26221 ttatgggtca ttggccgact gccggttctt gtacattatc gtcacgtcgg cggcaaagct
    26281 gaagggcggc gcggagcacg ggcggggcgg gggcgatcga gcagcggccg gccgggggct
    26341 ggaccgtccg ccagcccgca gcgcgccgtt gggccggacg cggcaacttt cctgcatcaa
    26401 aaatgggact ctgcctgtat attatgtgta tgcggttttt tggcgcagta ctcccccctc
    26461 gacgggggtt ggcggccggc cggctggccg ggtttgtggg cctcgcggcg ctgctcgcgg
    26521 gactgcttct cccccgcgcc tccctgtctc agccgacgat cgacctctcc cggcagcgga
    26581 tgccgcaggc gatgcctggc cggggcgcca acccgaaccc ttcggcgctg aaggtcgccc
    26641 gcctccacta ctccggcggc ggcgactggt actgggggag ctcagcgctc cccaatttcc
    26701 taacatacct gaaccggcag accgatttcc cggtcgacac gatggagcgg gtggtgacga
    26761 tcatggatgc cgacctgttc cgctacccct tcctgttcgc gaccggccac ggggtgatcc
    26821 ggtttgaggc cgaggagcgg gaacgtctgc gccggtatct cgaggggggc gggtttctgt
    26881 tcgtgaacga ttcctacggc atggaggaga cgttcaagaa ggagatggcg gagctgttcc
    26941 cggagcggga accggtggcg gtgccgttcg accatccgat ttaccgctgt ttctacgatt
    27001 ttcccaacgg tccgccgaag atccacgagc acgacggcca gcccccgcgg gcgtgggcga
    27061 tcattctcca cggccggatc gtgctgtatt tcctggtgga atcggacatc ggggatggct
    27121 gggaggatcc gcaggtgcac aatgacccgc cggaactgcg ggaacaggcg ttccggatgg
    27181 gcgtgaacct ggtgacctac gccttgttgt actagtcggg ctgcgcgaga ttacctgccg
    27241 ggccggcggc ctacctgtcg atagaaagaa tgcgcggctt cgccggcgaa gccgccgcct
    27301 gagaccgaac accgagggta tcgctatgac cgagttgacc tcccgccgcg cgctccttgc
    27361 caagctcacc ggggtgctgg tgcgccagcg cgcagtgctg tttgtatccg gcgtcatgtt
    27421 gacggccgcc gcggtgacgg cggcggcgat cgggctgtcg ctgtgggcga acgtgatgat
    27481 tctgccggtg gcggtgaagc tcccggtcct gatcgcggtc ggggcgggaa cgctctatct
    27541 gttcgggcgg tacgcggtcg cccggctgtt ttcggggtcg atcgatgcgg tggcggtcgc
    27601 gctggaggag cggaaccccg agctcaaggg gcggctgatc gcggccattc agtttgcgcg
    27661 gatgaaagac gcggggtgct actcggcggc cctgatcgag gcgaccgagc gccaggcgct
    27721 cgcgcgggcc cagggagtga atttcaacca ggcgctctcg ttctacccgg tgctgcgcag
    27781 cgggcggtat ttcgcgaccg cggccgccgc ggccctcctg gtcgtggccc tcctgccggg
    27841 gatgttccgc tactcgttcc tcgtgttctc caacccgacg accgaaatcg cccctcccct
    27901 ggcctacaag gtggtgccgt tccccgggac gaccgagtgg gtgaagtacc gggacatcga
    27961 aatcggggcc tccattttcg gtgaacggct cccggaaagc gcggtcattc accaccggct
    28021 ggtcgacggg aactggcagg agacggagat cgacttgcgg aaggtgcggc gggcgccgat
    28081 ggtcaacggc gactcggtgc ggattgcgac cactctccgc cagatcaacc ggtcgttcga
    28141 ctactatgtc gaggcgggcc gggtcgccac cccggtccag caggtgagcg tggtcgaccg
    28201 cccgagggtc aacggcctca agctgtcgat cttctacccc gactacaccg ggctggcccc
    28261 gactgtgatc gacgagaaca acggctcgtt ctcggccgtg gtcgggtcgc gggtgaacct
    28321 ggcggtcgag accaacctcc cggtcgcgcg ggccgagctg gtgtttgacg attccagccg
    28381 gacgccgctg gaggtcgacg ggcggcaggc ggaaacggcg ctccgcgtgg acaagtcgct
    28441 cggctaccat gtccgcctga tcgacggcct gggcgaggaa aaccccgacc cgatccagta
    28501 ccacatcacg gccgtgcccg acgaataccc ctcgatcgac gtcctcttcc ccggcgtcaa
    28561 cgtcaacctg agcgacgaaa tggtgctgcc gctgaaggtg cggatattcg acgactacgg
    28621 gtttacctcg ctggtgctga aatacacggt ggtggcgcac ggacaggcga gcgaggagaa
    28681 cgtggccgtg ctccatttct ccgaccggat caagaccgaa ggggagatcg ccttcaactg
    28741 ggacatggac aagctcaaca tgtacccggg ggattacacg atctatcgct tcgaggtggc
    28801 cgacaacgac gtgaactcgg ggccgaaagt gacggcgtcg cggcagtacg tggcgcggat
    28861 cccctcgctg gaggaaatca tcgccgaggc ggagggggaa agctcgcggc gcgtggacaa
    28921 cacccggcaa ctgctccagc agggggagga cctgtcggag cggttcaaga acatggcgcg
    28981 caagctcaag gcgcagaacc gcgacgagat gaaggcggag tggcagcacc agaaggagat
    29041 ggaggcgctc gccgcccaga accaggagct catcgagaat atcgagaaaa tggccgagga
    29101 gatggacaag tcggtcgaga agctcgccga caacgcccag atgagccgcg agattgtcga
    29161 aaaactccag cagatccaga agctgttcga ggaagtcgcc acgccggaaa tgaaggaggc
    29221 ccagaagaag ctgatggagg cgctccagaa aatggaccgc aacgagatcc agaaagccat
    29281 ggagcagttc cagctctccc aggaggaaat gctccagcgg ctggagcgga cgctggccct
    29341 cttgaaacag atgcaggtcg agcagaagat gcaggcaatg atgcggcagg cggagcggct
    29401 gctcgaacag cagaaccagg tcaaccaggc gaccgacagc agcgcccaga gcgcgctgcc
    29461 gcccttggcc gaacgcgaaa cggacctgaa aaaagatctc gagtcgctca aaaaggagac
    29521 cgccgacctt cgcaagctga tggacgaggc gcagatgaac cagagcgagg aggcgcggaa
    29581 attcgccgag gcggtcgagc agaccgacgc cgaccggaac atggaaaaga tgagcggcgc
    29641 gctcgcccaa cagcagcagc agccggccgc cgccgaggga aaacaggcct cggccaagct
    29701 cgcccagatg ctcagctcca tgcagcagca gctcgccgcc atgaacagcg gccagaacga
    29761 agcgctccgc aagatgatgc ggctggcgat cgacgacgcc aactacctgt cgcagtcgca
    29821 ggaagagctg ctcaagcggg cggccgaggt caccacccag tcggatatcc tgcgcgagct
    29881 cgcccagtcc cagcaggatc tcatgaccgc gtgcagcggg ctgaagggcc ggatcgcgga
    29941 gctggccaag gagtcgccct ttgtcgcctc cgaactgggg cagatcatga accaggcgac
    30001 ggcgcagatg gacatggcca tgctggggct gggcgaccgg cagggaccgc aggcgatcgc
    30061 cctccagcgc gacgccatga ccaacctcaa caaggtgtcg ctgtacctca tggaggcgat
    30121 gaaccagcag aaccagtgcg acaagggggg cagctgcgac aagaacatgg ccaagctgca
    30181 gtcgatgtgc aacaagcaga accaggtgaa ccaggagacg cagcagacct gcaacaaccc
    30241 gtcgttcaac ccggccagcc agggccagca gacgcgcgag gcgctccagc ggctggcggg
    30301 cgagcagggt gcgatccgca agtcgatgga agagctggcg caggagttcg gcgactcccg
    30361 ccaggtgctc gggcggctcg aggacatcgc cgacgaaatg agggaaatcg aggaggcgct
    30421 ggcggaaggc gaggccggac cggaaacgat cgaacgacag ctccgcgttt actcccgcat
    30481 gctgcaggcc tcgcgctcgc tccagcgcaa ggatttcacc gagcagcgca aggcggtcac
    30541 ggcggaggag cagatctacc aggtgccgcc ggcgctcccg gccgagctgc tcaacgaccg
    30601 gaccaatctc gaggaccggc tgcggcaata tctcggcgag cactaccctc ggcagtacga
    30661 ggaacaggtg aaagcgtatt tcaaggcgct gctgcaaacg gaggcggcgc cgcaggaggg
    30721 cgggcggtga agcgcaccct cgggcggctg atcgcggcgc tcgcgctggc cgcaggactt
    30781 ctgggcgcgg ccctcgcgca agcggcggag gaaccgaaag aggtcacccg gccgccgggc
    30841 ggactccgcc tgtacacccc gcaggatcag caggcgcagg accagcaaat gctgaacatg
    30901 gtgcgggaga tgatgcgcag caagaactac gagggagcgg tcggcctcct cgaagctatc
    30961 gaccgccgcg accgcgggaa catggtggtg atgaacctcc tgcgcacatg ctacgaccag
    31021 ctcgggcagc cggggagctc ggaggtggtg atccggcgca tgctcgagat caccccgaac
    31081 agccacgtcc tgcatgtcta cctggcggag gcgctggtgc gccaggagga cacggccgcg
    31141 gggcggcagg agtacgcgac cgcgcttgag ctggtgccgg agaagaccaa cccgggcatg
    31201 ctgtcgcagg tggtcgtgag catgcaggag gtggggctga gccgcgacgt cctcgctctc
    31261 atcgagcgcc tgcggagaga gctcggctcc ccgcggctgt tcgccaagga agcgggcgag
    31321 atattcgagg cccgcgagga gtaccgccgc gcggccgagg agttctacgc ggccaccgcc
    31381 gatacctcgc ggacagggat cgaggccgag accaagctgc agatgatgct ccagttcccc
    31441 aagtcggcgc cggccgcgct tggggttctt cgggagcaca ccgcggacac cctggcgacg
    31501 gcgaaagcgg cgcagattct cgccggccac tacctccaag cgggcgacat ggaccaggcg
    31561 atgcactaca cgctgctgcg cgattcgctc gcggggacca acgggacggt gctgctgtgg
    31621 ttcatgcaca tctgcagcga gcggggtctg tacgcgcaga cgaccgtggt gggggcggaa
    31681 atcctgcggc gctacggcgc cagcccgctg gtggccgagg cccgctacct ctacgccgag
    31741 gcgctcacga ggctgggacg gtacgccgag gcggtcgccg tgtacgattc gatcgtggcg
    31801 gcctacccgc gcgtccagga caaggccgag gcggcctgcc ggatcgggca gatatatctc
    31861 aactacctca acgagccgcg gcgggcgctg cggctgttcg attccgtggc ggccgcctac
    31921 cccggggggc tggggtattt gcgggccctc ctggcggcgc ccgacgccta ccttcgcctc
    31981 ggcgaactgg acagcgcccg gacggcactc gggcggctgg cgcagcggcc gctcatagaa
    32041 gaagcggccg aggaagcggc cttcaagctg gccaagatcg atttctacga gaagcggttc
    32101 gacacggcgc gggcgggctt caacaaactc ctcgtggcct accccaaggg gttctatgtc
    32161 aacgacgcgc tgcaaatgat gctgctgatc gacgaggcgg ggtcggaacc gctcctgtcc
    32221 gagttctccg acgccctcct cttcgaggag cggcgcgagt acgattcctg ccgggcggag
    32281 ctggagcgga ttgccggggc ggaccacccg gcgctcagcg acgacgcgct ctaccggctg
    32341 gccgatttga gcatcgcccg gttcgattcc gcggccgcgc tggagtatat cgagcggctg
    32401 gccgggcagt atcccgagtc atacttcgcc ccgtggggac tgaagctcaa gggggacatg
    32461 ctcacggccg acccccggcg gcgggaggag ggcaaggcga tctaccgccg gctgctcgag
    32521 gagaacgcga actacccgtt cacctcggac gtgcgcaagc ggctccgcga gctcgaggtc
    32581 gatccgcgga cgggctgacg gcgcgcgttg ccggcctcag taggtctcca gcttcttgcg
    32641 cccctcttcg agcgtgcgga cgcggcgctc caactggaac accttgtcct ggtagctctc
    32701 caccttcttc tccttcatct tggtcgtcgt gcgcaccagg agcgcgagcg acacgagcat
    32761 gaagaagacg gaattgacct ggcggaagat catgcccatg ttgaacacgg aatccagaaa
    32821 ggccaggatt cccagcacga agagcaaact gcaccataca aacatacgtg cctccgaatg
    32881 cggcccgggt cccgcggccg gtcccgggcg taaaattaga ggttgtgccc ggcagggccg
    32941 ccgttatatt taccttttca agggacggtc ccttgcctct agtgtatcgg taagccgaga
    33001 ggactcgttt aggaggagac ggtggctgac ttatctctga cggacccgga caagttcgac
    33061 cggacgaacg ccgtggcggc gggggcggtg tttctggtgt cgttcattac gtacgcgctg
    33121 acggtgcagc cgacgttttc attctgggat tgcggcgaat tcattgcaac gtcgtacatt
    33181 ctggggatcc cgcacccgcc ggggaccccc ctgtttatcc tcctgggacg gatcttctcg
    33241 gtcatcccct ttgtggaaga tatttcccac cggatcaact acatctccgt gattacgtcg
    33301 gcgttcacgg ctatgttcag ctacctgctg acggtccggc tggtgcagta ctttttcggg
    33361 gagcagcggc gggagttgct caaccgggtg atttcgtacg tgggcggcgt ggtcggcggg
    33421 ctgttcgtgg ccttctcgcg gaccaactgg gggaattcgg tcgaggcgga ggtgtacgga
    33481 gcggccctgg ccctttcggt catgatcatc tggctggccg tgcagtacca cgacgcccgc
    33541 ggaacctcga aagggatccg gctgctcatc ttgatcttct atctggcgat gctggggatc
    33601 gggctgcaca tgacggtgtt tctggtggtg ccggtggccg ccttcttcct tctgttcaac
    33661 cgggacgcgg tgccccgcga ctacgcctat ctggcggcct ttgtgggact cgagctgctg
    33721 ctgattgtgc tcttttcggg cgaccggggc gggccggtgg cgttctacct gaccacggtg
    33781 ctgttggcgg cgctgctggt ggtcctgctg taccgcaaga tcaactgggc gatcctgatt
    33841 gcgctggcgg cggtgtcgac ggtgatgatc agtttctcgc tgttcatgga ggcgctgccg
    33901 tttttcgtgg tgctgatgat tgggctggcg gtggcggcgc accggcgcgg ctggcggttc
    33961 cagtggttga ccgggctggg gctgatcgtg gtggcggtgc tggggatttc ggtccacgcc
    34021 ttcatcccga ttcgctcggc ccacaacccg cggattgacg agaacaaccc ctcacgcaac
    34081 tggtcgacct tcgtgaagtt cctcgaccgc aagcagtacg gatcggagtc gatggtcgac
    34141 cggatgttcc accgccgcgg cacctgggag aaccagttcg gccaccaccc ccacatgggg
    34201 ttcttctcct acttcgagga gcagtactcg ccgaaagact gggggttcgt gccgtttctc
    34261 gggctgggac tgctcggggt gattgtcgcc gtgcgcaagc gccaggaaat cgggctgccc
    34321 tacctcgtgc tgctggtggt gacctcggcc gggctgatcc tgtacatgaa tttcgccgac
    34381 ggcacgcagt acgatccgcg gaccggggac gcctacctcg aggtgcgcga ccgggattac
    34441 tttttcacgc ccgcgttcgt ctttttcggc atcgcgatcg gcatgggagt gtcggcgctg
    34501 atgatgctcg tgcgggaggc gctggcgcgg cgcaatccgg cgatcgagcg gacggcggtg
    34561 tacgcgatga cggcgctggt gttgctgccg gcggtgccgc tggcccacaa ctaccacgcc
    34621 aacgaccgct ccgacaacta cctcccctac aactatgcgg ccaacctgct cgattccgtc
    34681 gacaagaacg ccatcctctt cacctcgggc gacaacgaca ctttccccct ctgggcgatc
    34741 caggaggtgt acaactaccg caaggacgtc cgggtggtca acctctcgct gctcaacacc
    34801 gactggtacg tctaccagat gaagcacttc tacaatgtcc cgatctcgct ggaggacgac
    34861 cagatcctct gggaggacgt ggaggtgcgg ccggggctga cgctgccgat gccggtgaag
    34921 gaattccggg accgtccgcg caaccggacg acctggctgg cggccgtgga gccgtacaac
    34981 gggcagatgg tcaaggtgca ggacatgatg gtcgacgaga tcgtggtcga gaacaggtgg
    35041 aaggatccga tctttttctc cagccaaccc tatgattctc cgctggatct gcagaagcga
    35101 acggcggcgg tgggcgtggt ctaccgcctg gaccgcaccc cgcccgagcg gctgatcgac
    35161 gccgggcgcg gctacgacct cttcatgcac acctaccgct tccaggggta cgaggactcg
    35221 aaagtgtacc gggacgagaa cgcgaccggc gtgtttatcg gctacggcat caacgccgtg
    35281 cggctctggg atgaattcga gaagcgcggg cagctggact cggccatggc cattgcggac
    35341 aagatcatga aggagtaccc cgagtacgcg cagatgtaca tgatcgtgtc gaacgaactg
    35401 cgccagcagg gcgacacggc ggcggccgat tcgcttctgc gccaggcgca cgacacgctc
    35461 accgcgttct gccgctccaa cccggaaaac ctgttctacc tgagcgacct cgggctcatc
    35521 aaagtcagca tcggctcgat ctccgggcgc caggatctgc tcgacgacgg gctcgctctc
    35581 atgtgggagg cgttccgggc caaccccgaa agcggcacgg ccttccggaa actcttctac
    35641 gccctcagcg accagcggcg ctacgcggaa atccaggagg cggcccgcct gcatgcctcg
    35701 tatccgcgca atctcgagga tccgttcctg cagcagctcc tcggcctgac ccagtccacc
    35761 ccccgcatcc ccccgggggc gggccagtag cggacccggc cgccggaaaa caaaaacctc
    35821 ccgcacgccc gtgcgggagg ttgtttcttt cacgcaaccg gccggcctat tcggagagct
    35881 tgtaaccggc gctgcggcgg agcctccccc ccacctgctc catcatgtgt tcctcgagga
    35941 aatcggtgcg cacggcgacc gggtcctcgt tgaaccggga aatgacccgg ttgacctcgt
    36001 gatcctcgta gagccggttc ggttcaaact gggcgagaat gtactcgagg aggtatttcc
    36061 gtttggtgcg gcggcttggc ggcaccgtct cggtttcggc cgagcgcttg agacggatat
    36121 tccggcgggg ggccggggcg gccgcagccc cgttgcggtt ggagcgctgc tgcagccact
    36181 cgtacacgga ctgctccggg atgcgccagt ctttcccgat cttgtaggcg gtgatttcgc
    36241 cggcctggac tttgcgcgtg atgacctgga cgttcatctt cagcttgtcg gccagctcgg
    36301 tggtggtgaa gaactcggtg ttctttatga cgccctcgta gtagtccact tgcgctctcc
    36361 atcatcttta ggcgttgcgg aacgtgtcat atcctcgcac agagtagtgt agttcgcact
    36421 acttttcggt aataacggat cggcggaggg caattcaaca aaaactctcg gaaaaaccga
    36481 ggcccatccg cgcggatgcc gcggtcgcga agagccggca gaccgcccga tggtcggcat
    36541 cgaccaaaat ggtcgatgta acatgctgaa aaacaacagc ttggcgcctc cctgacggcc
    36601 gcggggagct cgcgggaagg cggggcgggg acgttttttt ttgccgatta ccgttgctcg
    36661 ggattcgaat cgatgcgtat attttttgag aagccaggcc ttttcgattc gaggtgattc
    36721 cggcggtatc ccgccggttg cggttcacgc gctggacccg accagatcga tttcgaacct
    36781 tccgattgcg gctgcacgcg gcacggcctg gctgacatcc tccgtctgga ggcaatcgtg
    36841 aaaggagagc cgaccatggc agcgatgcaa atgggagcac gggccccggg gcagcagggg
    36901 aagcgcacac gggtcaagta catcctctcc atcgacaaac tcacgcaatt atccgaggcc
    36961 gagcgcaccg cgctcaagga agtggcgcgg cgttacgcct tccgcaccaa cgactactac
    37021 ctcggcctga tcgactggtc ggatccgcat gatcccatcc gccggttggt cattccggcc
    37081 gtcggggagt tgcgacagtg gggtcatctg gacgcctcca gggaggggaa ggtcacggtg
    37141 cgcaaggggg ttcagcacaa gtacagcagc acggtgcttc tcctcgtcaa cgaggtatgc
    37201 ggctccttct gccggtactg ctttcgcaaa cggctgttca tgaacggaaa tgacgaggcg
    37261 gcgtacgaca tccgcccggg gatcgagtac ctccgcaccc gcccggaagt caacaacgtc
    37321 ctgctgagcg ggggtgaccc gatgatactt tcgacgccgc agttggacgg gattctcacc
    37381 gcgctgcgga gcatcgcgca catccgcatc attcggatcg ggtcaaagat gccggccttc
    37441 aatccttacc ggttcctcga cgacgatgag ttgttcgcga tgctccggcg ccattcgcaa
    37501 cctgaccgcc gcatctacct cgtatgccat ttcgaccatc cgcgcgagct gacgcccgag
    37561 gcgcgcgagg cggtccggcg cgctatcgag gccggcctca tctgcgtcaa ccagaacccc
    37621 atcattcggg gggtgtcgga tgatccggag gtgatggcgg agttgtggaa cgagttgtcc
    37681 tacatgggga tcccccagta ctacgtattc cagggccgtc cgaccgccgg caatgagccg
    37741 tacgaggtcc cgctggtcga ggcgtactgc agcatcgaga gggcgaagag gaaatgctcg
    37801 gggctcgcca agcgcatcaa attctgcatg tcgcacgaat cgggcaaggt cgaaatcatc
    37861 ggggtcgacc accggcatgt ctatctgaaa tatcaccggg ccaaacatta ccgcgacgaa
    37921 cagcgcctgc tcgtgtgcca ccgcaacgac caggcgtact ggctcgatca actgaagccg
    37981 gtcgagggat accggaacga gtactactcc gatcagggat tgcggctgta cggcttttcg
    38041 tagggccacc catccccatc gcggatcccg tgaatgacgg ctgtcgcagg gcagccgttc
    38101 tgctttggcc ggagagcgtg ctttcccctt ggtgttcgcg ctaaaatagg tatctttgtc
    38161 tggcattaca gcctgatgaa caacaggttc cctgccgaag ctgaacatat gcagcaggat
    38221 caagctctca acattgaggt gtagtatgcg cagaggtctc gtctcctggg cggcgctgct
    38281 gtgcgcgggc atcatcgccg gccgcgcggc ggcggttcca cccacgatca gctaccaggg
    38341 ccgtctcacc gacggcggtg cgccggtgcc ggacaatacg tatgaaatcg tctttcgcat
    38401 ctacgcagcc gacgccggcg gcagccccct gtggcttgag acacaggaag tggcgaccgc
    38461 ggacgggctg ttcaccgtgc tcctcggttc ggtcgcgccg atcggcgagg gcgtgttcga
    38521 cggatcggtg cggtatctca ccatgcagca ggagggcgcg gccgaatcgg cgccgcgcat
    38581 cccgatagtc agcgtgccgt acgcgatgac ggccgggagc gtggagggag cggcagccga
    38641 ctgcagcgac tgcgacacga tcttcctcaa cgcgatcggg ccggacgaac tccgggccgc
    38701 ggtcgacacg gcgctcaagg tgaccgacac cggcgcggcg gcgcgggtgg gcctgcgggc
    38761 tcgcctggag acgtccacga ccgacacggt gtccgccatt cgggggatcg cctacaacgg
    38821 cggctccggg cgaacgcagg cggcgtcgtt cgacgccacg ggcgaaggcg ggggaccggt
    38881 gcacggaatc aacgtgcagg cgacggggaa cggcagcagc tacacgacca tgggtgtgac
    38941 ggcgcaggcg actaatgacg ccgccgggac ggcgcgcggc gggtactttg cggcgacgaa
    39001 cgacggcttc ggcgatcatg tcggagcgat cgggcagagt tacagcccca acacgagcgc
    39061 tgccaagagc atcggactat ggggcaatgc ccggcattac gggataggcc ggtgtgtcgg
    39121 gggaatgttc gccgccgccg acggcgccaa cacgttcggg gtggatgcct cggccacgtc
    39181 ggccaatgcc gactcgacgt gcgctgtccg cggctacgcc aagaattcgg cgaccggagg
    39241 gacgtccgat gcgatcgggg gagtcttcat cgccgactcg gccggaacgg catggaaatg
    39301 ggggtcgatt ggcttggcct acggcggcag cagagaagcc accggcgtgt acggctgggc
    39361 tgagacaaac gcgactgtat ccagggccac aggagtcttc gggcaagctt cgaacaccgg
    39421 tcccggggac acacagggag gttacttctg gggacacggc gagtccggcg acacctatgg
    39481 ggcgaagact tttggctact ctacgacggg cagtgtctct ggcctctacg cacacggatc
    39541 cgtcaccgac aacgtctacc ctgtctacgg cgtgtatggc cgggtcgaca attacagctc
    39601 cggcagcttc aactccgagg cctacgccgg gtatttcgag aactgtgtct acaacgcctc
    39661 caacgcctac gggctgtacg gccatgtcta cgacgtccaa ggcatctacg cctgttacgg
    39721 ggtcgtcggc cgcacagccc acactggctc cggctccagc attgccggca gtttcgagac
    39781 cgactcgacg ggaacaggaa cagcttacgg cgtgcgggcc agcggcctgt ccaaaggttc
    39841 ggcgccggcc tacggcgtgt atggctcggt ggccagccgg gggaacggca atctctacgc
    39901 cggctacttc aaggtctccg actacggcac cggattgcgc tacggggtgt acgcgagttc
    39961 cagcgtctcc ggatacgccg gctacttcag cggcgacgtc cgggtgaccg gcgaccaggt
    40021 ggtggtcggg tccaagagcg cggcggtgga ggtggaaccg gaggattatc ggctggtcta
    40081 ctcgcaggag tcgcccgagt gctggttcga ggatttcggc gaggggcaac tggtcaacgg
    40141 ccggacccat gtggagctgg atccggtatt tctgcggaca gtcacggtca gcgcgcagca
    40201 cccgatgaaa gtgttcatcc agttgaacga cccggactgc aacggcacgg ccgtggtgcg
    40261 ggggatgacc gggttcgatg taatcgaact gcaaaacggg caggggagtg cttcgttcac
    40321 ctaccgcgtg gtagccaagc gcaaagggta cgaagacgtg cggctggcgc agatgcccgg
    40381 gcagacgccg gaggaggtgg aggcggaggc gaagcaggcg gaagcccggc tgcagaccga
    40441 ggaggaacag gcggcgcaga gggaaattgc cgaaagggat cggctcgccc agatccggca
    40501 ggaagaaagg gagcggcagg aagcgcggcc gttcggggat cgcgattagc gttcggcgat
    40561 tgacgggcag gacggaggca agacgcggcc ggcgataacg ccggccgcgt tgtcgtcagg
    40621 agcggctcgc taagtcgagc gggcaaagaa gaccgagcta ttcgtccctt ttcgcttggc
    40681 ggggggctga aatggggtat acctttaatt gtcatcagtt catctatccg ggacgtgtcc
    40741 tcatggccgt tacagaagct gtcggttttc cgatggctgt tcggaggtgc acgatgaacc
    40801 aacgcaggtg tcgattcgcg tatgcggaga cgatctccgc gctcttggcc gtgattttcg
    40861 cggtcgcccc agtattcgcc cagcagagca caaccggccc cgaaccgccg cccggcatcg
    40921 atcgcgggct ggcgccgccc gtacggggga cggtggtgct ggacgtaacg gcgtacctct
    40981 ggcaccacgg gtgcggcccg acagccgccg gcatggtgat ggggtactgg tcgatgaacg
    41041 ggtacgaaca gctggtcaaa tatggcgtgc cggctatgat tgccggcgat cacggggatt
    41101 cggtgtgcgg cgctccgatc ggcgaccact accacgatta ctcatgcccg atcgacggcg
    41161 ggagtccgat tccggacatg tcggagctcg ggggggcgca tacgagcgac tgcatcgcgg
    41221 acttcatgag aacatcctgg agcgccgcct acaacctgta cgggtggagc tggtttgtgg
    41281 acgtggggca ggcgctgctg gcttaccctc cgtacgcgga tccgacctac acgcattttc
    41341 ggatgcggga gctggatttc tcgagcgtgt cgtttaccga ctacaaggcg gagatcgacg
    41401 cggggcgacc gaccgtattc ctggtggaca ccgacggcaa cggggtgacg gatcacttcg
    41461 tgaccggaat tggctactcg gaagacccgc cgaagtatgg ggtgcacgac acgtgggacc
    41521 agtcgatcca ctggtacttg tggcgtgcca ttgcccccgg cgtgagctgg ggaatatacg
    41581 gcatgcattt tttcggcccg gctgtcagtt tctccgctgc cccgcgcttc ggggatatct
    41641 cgctcgaggt ccagttcacg gggcaatcgt ttgatccgcc agattcatgg cagtgggatt
    41701 tcggcgatgg ccagtcctcc tcggtgcgat cccccagcca tcagttcacc gccccgggga
    41761 tttacgatgt ctcgctcacg gcaaccttcg ccggcgagcc gatttcctct ctgcgcgagg
    41821 atttcattat tgccttggcc gactcgcttg ccggtcagga tcaattggtc gagggcggca
    41881 gcgaagtcga agtcgtgatt tcggcctcga acacggtccc gctcaaggag atactgattc
    41941 ccctgtcgta cgcggggaca ctcgacctga ccctggtgtc gttctcgact gcgggttgtc
    42001 ggacagacta tttcgaatcg accggctatt ttgatttcgc gccgggacgg gatcaggcga
    42061 cgatctacct catcagttca accgcaggta cttcgccgga gcttgcaggc gggagtggcc
    42121 ccgtggtcag gctcacgttt tccaccggct cggcggggcc gggggattcg gcggttataa
    42181 gtatgtccgg gtattcgacg tactctccgc gattctccgg ttctctgctg gcctacacgc
    42241 cgaagacctt atccccggtc ctcaggtatg cggagtgctg cgtaggtgaa cgtggcgatg
    42301 tggacggcga cgggtcagtc aagatttccg atgtcaccgc cctggtctcg tacttcttcc
    42361 gggatggaga accgccggcg tgcaatgacg aggcggacag tgacggcagc ggagttctga
    42421 acatcgccga cctcacgtat ctgatcggct acctgtttcg gggcggaccc gtaccggcgc
    42481 tgtgtccgag atgatcggaa actcgctgat cgttggccgc ggacgctctg tccggatcgc
    42541 cgctcgccgc gaggcggggc cggacggtgc gaccttgatc ggatatttca gtgtagtgac
    42601 ctctgcagtg agagtttgcg gcgaagttat tccctgtgaa tcaggttagc tgatgcctgg
    42661 gccgatggca ggatgagcac gtcggcaacg gtgacgtggg gcgggcgatc ggcgacccag
    42721 acaaccgcct ccgcaacatc atcgccgctc agcggcgtga acccctggta gaccttggcg
    42781 gctcgctcgg tgtcgccgtg gaagcggacc tcggagaact cggtgcggac ggcgccgggg
    42841 tcgacggtcg agacccggat gggggtgtcg accagatcga ggcgcagtcc cttggtgagg
    42901 gcatcgacgg cgaatttggt ggcgcagtag acgttgccgt tcgggtagac ctcgcggccg
    42961 gcgattgacc ctatattaat gaggtggccg cgcctgcggg cgaccatacc ggggatgacc
    43021 gcccggctga cccacaggag gcctttcacg ttggtgtcga tcatctcctc ccagtcccgg
    43081 taatccccct cgtgcagttt gtccaggccg cggctgaggc cggcgttgtt gaccagaatg
    43141 tcaatttccg accacggatg agggaggccg gcgatcgccg cgttgacggc tgtccggtcc
    43201 cgaacgtcga gttccagcac atgcacatcg gttccgagcc gggagacaag ttgtctgagc
    43261 cggtcgacgc gccgggcggc cagcacgagg cgggcgtggc gggcggcaaa tttacgggcg
    43321 caagcctcgc cgatcccgga cgaagcgccg gtaaccagca cgatcttgcc ggtgagatcc
    43381 tgagacatag aactcctcgc aagcagatgg tgtcaatcct ccgcaatgta ggtgtccggt
    43441 cggggaacgt caatgccccc accggcggat tcgggggcgg atagggggcg aaaagcgatt
    43501 cggtatcggg ggaaagccac agcggccgag gagaccgtct cgccaaacga ttattaagtt
    43561 attatctaat aatgttttaa ttctgacgaa gagtttgtgg ccggagggaa tcgttggcaa
    43621 ggcttatgcg cagaaggaac cggaaaagag tgaattcaag tccgtttcaa cccgatacat
    43681 gatatgtgga ccggtctcgg gtggacggat gcaaccggga gactggcgcc atgacacaga
    43741 acagcacgag cgcgaagaag cggtcggtac ggaacggttt gccgaagaag acgtactggc
    43801 acctgtacag cctgcagagc ctggagtcgg acaagctggc gaagattgtg aacaaaatcc
    43861 tgagccggga atatgtgctg attaagtgag attttagctt gacgcggtcg caaggcggga
    43921 gtagtttcga aacttatcga ggatcagacg ccccgctgtc ctcagatacc cgacaacagg
    43981 atgcaggact ttgcagcatg agtggattgc gacagcatgt ttaagaagaa actaacgttc
    44041 atgctcatac cccattcgat ggggatatcg cggcaggtac ggataccggt cgcggccatt
    44101 tacctggcgg tcggcggcgt gatgctgctg gtgttcgcca gtttcttttt gtcggcggag
    44161 ttttttgccg acaaggtgga cgcccgggag ctggcccggc tgcgggccga aaacgcggcc
    44221 ctgcaccggc agttcgagca gttgaaatgg aacctcaccg aggtggagac acggttcagc
    44281 cagctggtgg accgcgaggt gaagatccgg tcgctcttca gcttacccga aatcgacccg
    44341 gccgagcggc aactgggtgt cggcggtccg gcgccgccct cggcggcgat gatgtcgaat
    44401 gtccagcgtg aggcgacggt gacgggccag cagatcgacc gcctcctcac gctctccagt
    44461 ttcgagctgg agaagttcaa cgaggtcgag gatgagcttc tgaagctcag ggaccgcctc
    44521 gaccacacgc cttcgatctg gcccaccaac ggctggctct cccgaggatt cggcatgaaa
    44581 ttcgacccgt tcacgggcat gaagcagatg caccgcgggc tggatatcgc caaccggacc
    44641 ggcaccaagg tgatcgcgcc ggcggccggc cgcgtcaagt ttgtcgggca gaacggcggg
    44701 atgggcaaca cggtggtgat cgaccacggg tacggtttcg agacgcgcta cgcacaccta
    44761 tcggatttcc gggttaagcg ggggcagcgg gtggagcggg gcgacgtggt cgggctgatg
    44821 ggttcgaccg ggtattcgac gggtccgcac ctgcactacg aggtcctccg caacggcaaa
    44881 caccttgatc cgcgcgtgtt tatcctgaac gacaacagtt gatcggcggt ccccggcaag
    44941 cgaggaggcg tccggagccg ggcgcctttt ttcgtggccg attgaaccgg gaaccgtccc
    45001 ctccccgctc cttcgccggc actcatggaa ctttggccgg ggcatgcggt tatacctgac
    45061 aaagcgtacg gagcaaggca tgaaacgaaa tccggcgatc ccgcaccatc tccgcgaagc
    45121 gatccccccg ccggatgaac gcgatgaaga gcgggtcaaa gaactaatcc gggaaatcaa
    45181 gcgcggcaac caactggcct ttgccgaact ggtgcgccgc taccgggtgc aggtcgcggc
    45241 gctcgcctac aagatggtcg gcgactacga cgaggcggcc gatatcgcgc aaaacgtgtt
    45301 cgtcaaaatg tcccgcaaca tctggcggta cgatgagcgg cggaagttct acacctggct
    45361 gtaccggatc acggtcaacg cttcaatcga ctatatccgc aagcacagcc gccaccgcca
    45421 cgagccgctg gagaatttcg agaacgtggt cgagcaggcg gaccgacgcc cggatgcatc
    45481 gtaccaccgg cggcagatcg atgattacat ccgggaggca accgcgtcgc tcaccgacaa
    45541 gcagcgctcg gcgttcgtgc tgcgcgatgt ggaggggtgc cgaatcgacg acgtcgccga
    45601 catcatgaac atgccggaag caaccgtgcg ctggtacctg caccgggcgc ggacgaagat
    45661 ccggcgggag ctgcttcgga agtgcccgca cctgctcgcg ctgttcggga tcagatgacg
    45721 cgcccgcgag ccttaccgta ccttttctgt tggcgaacaa cgatcttaag gccggagagc
    45781 gggcttttgg cccgaggttt ggaaggaccg gtggatggcc cagaccgaag aacttcaggt
    45841 aaacatcgac gggatgcact gcgccgggtg cgccgcgaac atcgagcgcg gtctcggagc
    45901 gctggcgggg gtggcggagt gccgggtgaa tctggcgacc cgctcggcgg tcgtcgcgta
    45961 cgaccgggac gagacgagcg cgggggcgat tctccggacg atcgagaaac tcggctacgg
    46021 agcgcgggtc ggcgcgccgg acgtactcac cgccaaccgg aaagaattga gcgccgcgat
    46081 ccggcggttc tggccggccc tggggctgag tctccccctg atggcgctgg cgatggggcc
    46141 gatggtcggt ctcggcggga tcctggcgcc ggcggccgac gggatcgcgc aggcgctgct
    46201 ggcgggggga attctgttcg gcgccggtcg ggcgatactc ggcgacgctt tcgcacaggc
    46261 ccggcgaagg gcggccaaca tgaacacgct gatcgcgctc ggcacactca ccgcctacgg
    46321 atggagtctg tatgcgctga ttgcgaatct cgcgaaaggc ggggccgagc cgctcttttt
    46381 cgattcggcc gggatgatca tcacgctgat tctcctgggg cggttgctgg aggcgaaatc
    46441 gaaggggcgg gcgggcgagg cgatccggtc cctggttcaa ctgcagccgg ccaagacgac
    46501 cgcgatcatc ggcggcacgg aggtcgaaat cgacgccgcc gccgcgcagc cgggaatgct
    46561 cctgcgggtg cggccgggcg agcggatagc ggccgacggc cgggtggtgg agggaacgcc
    46621 cgcggtcgat gagtcgatgc tgaccgggga gtcgctgccg gtcgagaagc agccggggga
    46681 tgcagtgatc ggcgggtcgc tcaacggcca cagcgtgttc acgtttcagg tgacggcggc
    46741 cggcgcggac tcggtcctgg cgaagattat cgcgctcgta tcggaggcgc aggggaaaaa
    46801 agcgccggtg cagaagctcg ccgaccgggt ggcggcggtc tttgtgccga tcgtgctcgg
    46861 gctggcggcg ctcacgctcg cgctctggct tttgttcgca cccggaaacg acgtcatgat
    46921 gcggagcgtc atctccgtcc tgatcatcgc ctgcccctgt gcgcttgggc tggcgacccc
    46981 gacagccatc ctcgtcggga cggggcgggc ggcccgcgaa ggaatcatca tccggggcgg
    47041 agatgtgctc gaacggatca cgacagtcga cacggtcctt ttcgacaaga ccggcacgct
    47101 gacccacggg gagcttcagg cgaccggcgt gaccgcgctt aacggatacc gcgaggagga
    47161 gttgatcgcg ctcgtgggcg gggccgaaag tatgtcggag cacccgatcg gccgggcgct
    47221 tgcccggctg cgcgaagagc gggggatccc gccggcggag gtgcgcaaag ttgaagcccg
    47281 gcccgggttc ggcctgatcg gacagagcga gggggggcga ctcgtgatcg gcaaccaggc
    47341 actgctcgag caggaaaatg tcgctctggc cggccaccag gaggctatcg agcggacgac
    47401 cgccgccggc cacaccgccg tgctcgctgg attggacggc aagctgatcg gcctgttcgg
    47461 catcgccgac cgagtccgcc cggaggcggc ggaagtagtc agagaacttc agaagaccaa
    47521 gcaggtggcg atgatctccg gggacacccg gcgtacggcc gagaaagttg cgcggctggc
    47581 gggggtcgag catttcgagg cggagatcaa gccggaccag aaacgactgc tggtcgattc
    47641 ctaccgccgg gcggggggca cggtggcgat ggtgggagac gggatcaacg atgcccctgc
    47701 tctggcggcg gccgatgtgg gcgtggcggt cgggtcgggg acggacgtgg cggtcgagac
    47761 ggccgacgtg gtgctcgtac ggccggacct gcgcggggtg ccgaagatgt ttctggcggc
    47821 ggcgtggacg atgcgggtga tccgccagaa cctgttctgg gcctttgcct acaatgtgct
    47881 ggcgattccg attgcggccg gggcgctcta tccggcattc gggctgacgc tgacgccaat
    47941 gatcgcggcg gcagcgatgg cgttctcctc ggtctttgtc gttctgaact cgctccgtct
    48001 caaccgcgtg cggttccgct agcctcctcc tctcaacctc tcccccgtcc cggccgatac
    48061 taacggtgtc ccggcgcccg cacggccggg gtcagggttg attgcgaatg ccggcggccg
    48121 ccggcatttt tttgtcttcg cccggccgcc gatttctcct ttctactttt gccaatccga
    48181 ccgcggggtg ttagattggt ccgacgaagg agcgaggttg cgtggctcga ctgcatctga
    48241 cacccaaagg ggcgctcatc gcgtttatcg tgctcgccgc gacggccgtg gcgcagatgg
    48301 tctggtggat catcctcatg gcccacctgg tcgatgcgca ggtggaggtg gcccgccggg
    48361 tcggggccga cccggtgctg atcgaacaac tgcaccgcga ggaagtccgg cgacaggtga
    48421 tggtcggtct ggagggagtg tttttcctgg tgctcattat cggtggagcc tggttgatct
    48481 accggtctct ggtgcggacg gagcagctca agtttcacca gcagaatttc ctcatggcgg
    48541 tgacgcatga gctcaagacg ccgctggcct cgatcaaact ctacatcgat accctgaagt
    48601 cgcccaaaat accggaggaa aagaaggccg gggtcctgcc ccggatgcgg gaggatctcg
    48661 accggctgga gcggctggtg gacaacatcc tcgaggcggg gcggttcgag cggagcgggt
    48721 acaaactgca ccgccagccg accgatctcg gccgattggt ggaacgccgc ctgggcgccc
    48781 tggagcggac cccgaaacac aagccgctgc acgtaacctt ggacctggcg gcgggcgtga
    48841 tagtcaatgt cgactgttcg gcgctggaac gggcaattga cgcggtgctt gaaaacagcc
    48901 tgaagtataa cgacaaggcg gccgtggacc tggcggtcag tctggcgcgg cgggagggcg
    48961 cggtgcacct ggtgttcgcc gacaacggga tcgggttgga acggggggac ttgaacgcga
    49021 tcttcgagcg cttctatcgg gttggatcgg aggtgagccg ccgacatccg gggtcaggcc
    49081 tggggttgta tctcgcgcgc gagtttgtcc gggcccacgg cggggagatg gcggcggagt
    49141 cggagggacc ggggttgggg gcgcggtttg tcataaccat ggaaacggag ccggagaatg
    49201 aagaagatcc tgctggtgga ggatgatccg aatctcgccg acgggttggt gatgaacctt
    49261 gaggcggagg gttacgaggt tgtgtcgatt gccaacggca gcgaggcgct cgacgagttc
    49321 cgacggggga gtttcgatat cgtgctgctg gacatcatgt tgccgggaat ggacgggctg
    49381 gcgatctgca agcggctgcg ggccgagggg tatcgggtgc cgattttgtt catcaccgcg
    49441 cgcgggcaaa cggaggatcg gatcgagggg ctggtggcgg gcggcgacga ctacatcacc
    49501 aaaccgttcg atgtggcgga gctgctggcg cgggtgcagg ggattttccg ccgccaggca
    49561 tggctcgccg caggcgatga ccagctaggc gaggtgtacg agttcgacgg gcgacggatc
    49621 aatttcaaaa cgttcgaggc gaccgggccc ggaggcatgg cgccgctgac ccacaaagag
    49681 tgcatggtca tgaagtacct gatcgagcgg gccggcgagg tggtcagccg ggatcaactg
    49741 ctcgacgcgg tctggggata ccataccttc ccgaccaacc gaacaatcga caacttcatt
    49801 ctcaagctgc gcaaggtgct cgaggacgac ccgaaaaatc cccgctatat cgagacgatc
    49861 cggggagtgg gataccggtt ctcgcggcgg aattagccgc cggtcggacg cgcgaaccgc
    49921 cgggaaccgc cggcggttct atcgggaaaa ggaggcgacc gatggtaaca gtgcgcagag
    49981 cggaagagcg cggccatttc gatcacggct ggctggacac ctaccacact ttctccttcg
    50041 accgctacca tgacccgaac cacatggggt tcagatcgtt gcgggtgatc aacgaagacc
    50101 ggatcgcgcc ggggaccgag ttccccatgc acccgcaccg ggacatggag attatcacct
    50161 atgtcctgga gggggcgctg tcgcacgagg acagcatggg caacggaacg gtgatccggc
    50221 cgggtgaggt acagaaaatg accgcggggc ggggcgtgtt ccacagcgaa cgcaatgacc
    50281 tgccaaacgg tcggacccac ctcctccaga tctggatccg gccgtcggag aacggcctga
    50341 cacccggtta cgagcagatt aggttcgatg acgaggcgcg ccgcaatcgg ctgctgttga
    50401 tcgggacatc ggaccgctcc accgacggga aggcggtgca catcaaccag gatgcgcggc
    50461 tgtacacctg ccggctcgat gcgggcaatg cggtacgcga accgctggcg caagggcggt
    50521 atgcctgggt gcaggtcatt cgcgggaagc tgttggttaa cggccatgaa ctcaggaccg
    50581 gggacggggc ggctctcagc ggggaacggg aggtggcggt ggaggcggcc gaggggagcg
    50641 agttcctctt atttgacctg gcgtaggatg gcgccgaccc ggcggacagg attctagatg
    50701 ccggatggcg gccgactccc cgaagggtgg gaaccggcat cccgcacatt ggccggatca
    50761 gctcctctct ctgttcgtat catgttgcag ggtcctctct tgaccgataa ggtgaaggag
    50821 gagacaggag tggaatgggt cacctgaaac gctaccgaca aatcgccggc gcactggtaa
    50881 ggcacgggct gcactacctg gtgggagtgt tcggcctgga gcggtttgtg ccgtttcaca
    50941 aggggatgct ggcccggggc cgcccgagcc cggggcacac gccggccgaa catgtgcgga
    51001 tggccctgga ggatctggga gccactttcg tcaaactcgg gcaaatcctg tcgacccggc
    51061 cggatctggt gccgccggag tatctcaaag agctggcccg gctccaagat gacgccgcgc
    51121 cgatccccgg cgacgaggcg gaggggatcg tggcggagga gctcggggcg ccggtggcgg
    51181 agctgtttct ctcgttcgag cgggcgccgc tggccgccgc ctcgatcggc caggcccatg
    51241 cggcggtgct gcgcagcggc gaggaagtgg tggtcaaggt gcgccgcccg ggggtggtcg
    51301 agcaggtgac cgaggatatc gagattgtcc gcaatctcgc gctggtggcc agccggagac
    51361 tcggcccggc cgagcactac gatctgattg ggctggccga ggaattcgcc gagacgctgc
    51421 gcggcgaatt ggactacctt cgcgagggac gcaacgccga gcggttcgcc gagcatttcc
    51481 ggggggatcc ggaagtgcgg atcccgcggg tgtactggga gcaatcgact gccagcgtgc
    51541 tgacgctcga gcgaattcgg ggaatcaagg tcgacgacac ggcggcgctc gacggggcgg
    51601 ggatcgaccg caggcaactg gcgcggcgga tcgcgcgcgt gctcctgaaa atgatcctgg
    51661 aggacggctt cttccacgcc gacccgcacc cgggaaattt cttcgtggaa cccgacgggc
    51721 ggctcggggt gatggatttc ggcatggtcg gggtgatcga cgaggcgggg cgggagcggc
    51781 tggcgatgct cctgggggcg ctgacctcgg gggacgccga gcggctggtt gacacggtgc
    51841 tcgatatgag cgccggccgg gggcgggtcg accgggagga actcaagcgg gacatccagc
    51901 gtctgctgac ccgctactat gaactgccgc tggggcagat tcccctcggg cggatgctcg
    51961 aagaggcgct ggccgcagtc cgtcggcacc gcctccaatt gccctcgagc ctggcgctgt
    52021 tgttccgggc gctgatcgtg agcgagggac tggggctgcg cctcgatccc gatttcagcc
    52081 tcggtccggt gctcaaacca ttcgccgagc ggctgctcct gcaacgctac tcgccgctgc
    52141 gctgggcgca gcggatgggg cgggccatga tcgacaccga ccacctgctc tccgaactgc
    52201 cgcggcggct ccaccgcgcg ctcaagatgc tcgaccgggg gggattcgag ttcggcctgc
    52261 ggcgggagag tctcgaaccg atggccgacc ggctcgagcg gctggccaac cggatcgtgc
    52321 tcgggatgat cacggccgcg tttatcatcg cgctgtcggt gctgatgctc gtctacccgc
    52381 cggggggcgg ggaagggcac tggggcggga cggtgttcgc cgtagggttg gtggcggcag
    52441 cgggcctggg ggtgtacctg gcctggcgga ttatccgcac cggccggggg taaggacttg
    52501 ccggtcaatc ctctcccccc gcggtccgat cgacgcgccc ggagccgcgc ggcggagaag
    52561 ccttcttccc ggccgccggg atgccggaat gggaccggcc gggccggaaa aaggtcacac
    52621 gcgcacaatc acataatctg ttgactttgg ggcgggatag ggtataattg tctgctttgc
    52681 cggaaacggc agccctatga tttcgcttgt tgtcggactc gggaatatcg gccggcgcta
    52741 tgcgggcacc cggcacaacc tcggattcga agtgctcgac cgggctgccg cggcgctcgg
    52801 agcgccgcgg gcgacgagaa cgggtctgga cgagcgcacc gtgtgccggc gcggcgaccg
    52861 gacaatcgtg ctggcctggc cgaggacata catgaaccgg tcgggggacg cggtcgcggc
    52921 tcttttacag gattacggtc tccgtccggc cgaggtgctg gtggtggtcg atgatttcaa
    52981 tctgccgctc gggcgggtgc gcctgcggac
//
LOCUS       DAOPOE010000009        51640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_846_length_51640_cov_6.144170, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000009 DAOPOE010000000
VERSION     DAOPOE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 51640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51640
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_51640_cov_6.144170"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /gene="katG"
                     /locus_tag="PKM94_02410"
     CDS             <1..1713
                     /gene="katG"
                     /locus_tag="PKM94_02410"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HOD65519.1"
                     /translation="LESMGLKTFGFAGGRTDVWEPEEDIYWGPEAEWRGSTRYSGDRE
                     LENPLAAVEMGLIYVNPEGPNGNPDPVASGRDVRETFARMAMNDEETVALVAGGHTFG
                     KCHGAGPASHVGPEPEAAGLAEQGLGWKSSFRSGKGGDTITSGIEGAWKPHPTKWDMG
                     YLKVLFKYDWELVKSPAGANQWLAKDVAEEDMVVDAHDPSKKHRPMMTTADLSLKFDP
                     IYEPIARSYLENPRKFADAFARAWFKLTHRDMGPRSRYLGPEVPAEELIWQDPIPAVN
                     HRLITKRDAAALRAEILASGLSVAELVSTAWASASTFRGSDKRGGANGARIRLAPQKD
                     WEVNQPALLAKVLRTLERIQKAFNKSQSDGKKVSLADLIVLGGCAGVELAAKKAGFKV
                     TVPFTPGRMDASQKQTDAASFAVLEPKADGFRNYVKARYSVRVEELLVDRAQLLTLTA
                     PEMTVLLGGLRVLGANFGGSPHGVFTARPETLTNDFFVNLLDLNTVWKPTAPDEELFE
                     GRDRATGALKWTGTRVDLVFGSNSQLRALAEVYACSDSLEKFVQDFVAAWDKVMNLDR
                     FDLA"
     gene            complement(1981..2766)
                     /locus_tag="PKM94_02415"
     CDS             complement(1981..2766)
                     /locus_tag="PKM94_02415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD65520.1"
                     /translation="MGEPTPRFWEIFFEVFESLPRQGPGNRACAARALALCRDLPPSP
                     AVLDLGCGVGGQTLHLAELTSGPIVALDRHAPSIERLAAAVSRLGLSARVFPVVGDMA
                     DPGLPPAGFDLVWSEGALYNIGIAHALRVCHGLLRSGGYLAFTDAVWRKANPPPEVKE
                     SFDVDYPAMGAVPDILAIIAGSGFSLLGHFTLPDEAWWEDFYTPMERRIEHLRAAYAA
                     EPEALAVLDQLAQEPDMHRRYSDYYAYEFFVARRGKQMDRCRQ"
     gene            3269..4549
                     /locus_tag="PKM94_02420"
     CDS             3269..4549
                     /locus_tag="PKM94_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65521.1"
                     /translation="MREARFSQPCILAATAIMAALLAADLDACTAFIVRQGHQVLVAN
                     NEDWTETNVRVWFVPGSPGRHGAVYFGFADTPGGESREGGMNDEGLFFDGFGAPAIGD
                     SNPLNKPLYQDSVGFPDKALAECGSVEEVTALASQFHLPLHHAMWMFGDRYGNSIIIE
                     GEKAVAGDGRYQICTNFRQSELQGDIAPCWRYRTADSLLENMPALSVAYCRDILDAVH
                     LKITQYSYILDPNSGQIYFYHFHDYSQVVQVNLVEELTAGPHYIDMLPRFPRKLGVTL
                     LAEQGKACYLSFSMVFFAASLIVWAGRVLFRRIVRVKPTGSLAQRSPGAAAGLIAGVL
                     CAVIGAYLVVLLGYPEMIPYSQENASGHSPFLRFVLLGAPWLGAALLGASIYYGWAVW
                     KTRAWRLVGRAHYTLLALIASADVVIWVAWGIIP"
     gene            4860..5876
                     /locus_tag="PKM94_02425"
     CDS             4860..5876
                     /locus_tag="PKM94_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HOD65522.1"
                     /translation="MRVEGRSCTSFCLDNKGEAVFGSNYDNDIPEGMVFINKRGVSKS
                     GLMPGTTGQVATWTSKYASVTFTVVGHQLAWAGMNEQGLVMSTMALGETQNPEPDERA
                     PLHSPHWMQYLLDNCSSVNEILAADSQVRIFETVDHYLVSDAAGDCAVIEFLDGKTVM
                     HTGQGLPAAVLTNSTYAESAALLDGSGGESGKRRDPQPINGSLRRFGIAAERVQAFEP
                     AGAEDAVTYAFDILDKVGGDATLWSIVFDTHDLTVYFRTKSHRAIRYLSLNDFDLSCQ
                     APDLMLDIHEKLEGHIAEAMTVFSSDRNFAFLKNAIARREIPMSDEQLQAMRAILDSY
                     ECEQ"
     gene            6316..7137
                     /locus_tag="PKM94_02430"
     CDS             6316..7137
                     /locus_tag="PKM94_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD65523.1"
                     /translation="MISLTNEDGITCASLMVLQEDREAWAIVVSWKKGVDGLPDRRAF
                     HEALESSIGECVRMGALYLDSRVITASTGVDDALTSSRAALHRDFLSARGFVRGEDRV
                     EYQMDLAVALSMLEADEIEPSLVWECVNTECASALARAADLFSQASEGDPASNPGEDA
                     VGFVKALIEEKETVQAPERLQIGMCGNDPAAVLALKVYPSDGWSTIYYLGVLPVFRGR
                     GFGAAAMLQALHSLKAMGGRIYHDGTASGNAGALALFARLGQPPFRVMETWRLRR"
     gene            7525..8184
                     /locus_tag="PKM94_02435"
     CDS             7525..8184
                     /locus_tag="PKM94_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65524.1"
                     /translation="MVRPKLLSNAQKIMICLVIVQVTTSGVLAFDGQRNGFVLGAGVG
                     LSAISTLSSSYMGHSFSGSGTGVGMNAIMGYGISKHDVLAVGMNWAGYRSDYTGENAD
                     IAQGIVALTWTRYFGKPGKSYLVLAGPGAYFARSFSGVDISICWDCPFEDMPTARMPE
                     DWGFGLIGGVGRELSTQFQLVVYGAIGFPSGNVYVPDGSQAAKTVTAAHVNILLMWMS
                     F"
     gene            complement(8427..9176)
                     /locus_tag="PKM94_02440"
     CDS             complement(8427..9176)
                     /locus_tag="PKM94_02440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD65525.1"
                     /translation="MDTWKFYNITHRKHLVCNPTSEAKLAHLVGLLDLPRGAGVVDIA
                     CGKGEFLIRLAEKYEVRGLGVDISPYCVEDCKKRAADRLSTPNVTFTQMDGAEFRPEQ
                     PASLHLVSCIGASWVFKDHSETLRTLKSWAAPGGLVVVGEPFWLHDPSPQYLDASGLQ
                     KESFGTHADNVEAGEKHGLRFVYTIVSDQNDWDQYEGLQWLAADEYARQNPDDPDITE
                     LMNRVTSSRNMYLKWGRDTVGWATYVFRRDE"
     gene            complement(9579..11153)
                     /locus_tag="PKM94_02445"
     CDS             complement(9579..11153)
                     /locus_tag="PKM94_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD65526.1"
                     /translation="MCPGNLAPNRFFSSSHFVRDGSAILLTIALVKLCLHLVVNITGG
                     YGMFRDEFYYIACSDHMAWGYVDQPPLSIAILWLNRLLLGDSLFALRLLPAVTGAVVV
                     LLAGLMTRELGGGKYSQILASICALLAPLTLAINAHFSMNSFDTLLWTLAFYLLILIA
                     KHDLSRHWILLGIVLGLGLMNKISVLWLGTGLLIALLATPYRALLLTRRVWFAVALSV
                     ILFLPHIIWQIANNFPTLEFMSNALADKYVSVSPWDMFSQQVLNMSPVSFPIWFAGLT
                     YFLVSRSTRQFRVLPIIFLAVFLILIVSKNSKSEYLGPMSPMLFALGSFTVEKFVLWL
                     KWRWLRPVILASVILGGLVPLPLTLALLPVDTFIAYSRALGIGPSTPEKKELSELPQY
                     YADMFGWKEMTAAVAEAYARLTPEEQSKCVILCNNYGEAGAIDYFGGEYHLPRAISGH
                     NNYWLWGCQNSDGEVVIRLGGSEDAMKECYGEVIPAGIFTHRYCMPYENNQTIWICKN
                     RRASLITDWPDFKNFE"
     gene            11694..13190
                     /locus_tag="PKM94_02450"
     CDS             11694..13190
                     /locus_tag="PKM94_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD65527.1"
                     /translation="MRTTFTAALAACLLSALLYAQTPPPSAQVEGSWVKVACPFDSSK
                     ALLAVTCGRLKVPENYDEPKGRSIEIAFMVVKARKNIDPENPVLFLNGGPGQTSLFFA
                     ETLVTTPLIYDVVVDRDWVFFDQRGTGRSIPQLYCPDDQDSPFDLKKCRDGLIKQGID
                     LSQYNSARSASDMEALRKALGVNQWNLWGISYGSRLAMTMARYYPSSVRSIVHDAPGL
                     PGGQELVDDALGTDVALNKIFSKCAMDSACSSKFPELRSRFTAALLRLRQQPLLVGEK
                     RFDDHAVVQFFRHWFYPRGYSTYEHRLQNLLIFMDAAARDDGPLMDETIQRMRKEEGL
                     DKVRPPEPIYARQSIAQNLSVYCNEKKPFESMDEYQKAVASSEIVRSLLADFGAPSDC
                     TLWPSGEADSIENTRVYYDGPQLAFTGELDASSSGLAGYKIEMLYANALNVVFKNGYH
                     GQFPTELPNSEDRDYWLCALRLAHQFFADPQRKLDTGCAETRRLRLVK"
     gene            13654..15096
                     /locus_tag="PKM94_02455"
     CDS             13654..15096
                     /locus_tag="PKM94_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD65528.1"
                     /translation="MKTRMLKTSCLSLALLMLPLLMHAQETGTVASPETVFDHVWQTV
                     DRTYGQFGSKHVDWAALYRVYRPQITAETSDGDLHRILLEMLGHLNDAHVCIQTDTSR
                     TCCGLTEGLPRVGFSRELIESHYLKGTADTALDGRLLSGWLTDQIGYMYLRNFKSKPD
                     ELSAAIDGCMKGFAAASALIVDVRDNTGGKSENSELVADRFADRKRHFATSFDRYGEK
                     HDDYLPPTYRNIEPAGPIQFTGPMVVLTDRHTASAAEEFVLAMRVLPHVTIVGDLTEG
                     ALAAQYPERLPNGWTLWVAHKYTIDLEGVCWDGVGIPPDVRITNTQADIDSGQDRVLQ
                     FALLFLETGRPEPQMEPASLDGIRTSLVQEFKMNLDKSGFEFAVNDLRSKIKGGDQRY
                     FFSVVEMLELSREYVSRDEFKQAIELLEILRAEYPRFAHTYGLLAAAYLNSENQEAAR
                     TVVAEGERIKAMYSWEKPLLEKVKQALEEQ"
     gene            15227..15622
                     /locus_tag="PKM94_02460"
     CDS             15227..15622
                     /locus_tag="PKM94_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD65529.1"
                     /translation="MRLPFVRSYLLAAILLVPLAPTTSCGENDDYDRFACKVVTLLAP
                     EIVPDEPGAAVVVTQQGKVIYRRCFGLANLEHRVPITQETVFDLASCAKQSSVLKSYG
                     MGNSNDQSTVRIEWIDYIILISAAGLHCS"
     gene            complement(15989..16612)
                     /locus_tag="PKM94_02465"
     CDS             complement(15989..16612)
                     /locus_tag="PKM94_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOD65530.1"
                     /translation="MKDCPKSLKHEAERSARKRLLKHEHIAPLTRYVDAIISQHPDKS
                     IPYFDPLDGGTRAQCLFVLEAPGQNAVRSGFISRNNDDESAKNFFEINVEAGIPREKT
                     ITWNVVPWYIGTGKKIRPATADDIRAGLPYLNSLIDLLPNLQIIVLVGKKAQRVKRKL
                     ESQYDYIRIFECYHPSPLVVNTNPRNKQKIIEALRPVSHILSQAARA"
     gene            complement(16722..16798)
                     /locus_tag="PKM94_02470"
     tRNA            complement(16722..16798)
                     /locus_tag="PKM94_02470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16762..16764),aa:Asp,seq:gtc)
     gene            complement(17019..17092)
                     /locus_tag="PKM94_02475"
     tRNA            complement(17019..17092)
                     /locus_tag="PKM94_02475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17056..17058),aa:Val,seq:tac)
     gene            17389..18732
                     /locus_tag="PKM94_02480"
     CDS             17389..18732
                     /locus_tag="PKM94_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOD65531.1"
                     /translation="MAVDPSIFKAYDIRGTVPDQLNGDLAYRIGYALASVLKPRSIAV
                     GRDMRVSSDLLFEHLARGINDYGADVIDLGLISTDALYFAVGKFGYDGGVMVTASHNP
                     KEYNGFKVCRKEAVPLSGQEGLNQVLRFIQDGEEMPRSPSPGVVARKDILSEYADHCL
                     SFIDISRVREFHVVIDAGNGIAGRTVPPVLDRLPIRTTRLYFELDGTFPNHPASPIEL
                     ENLADLIRAIAETGADFGAAFDGDADRMFLVDRKGRQIGGDMVTAMVAKSLLKRHPGE
                     TILYNLICSRAVPELVERMGGTAIRTRVGHALIKPLMKKHNAIFGGEHSGHFYFRDNW
                     FADSGLIAFLVCLELLSEERRPLDEVVRDLDPYVRSGEINSRVADQKAKIEAIAEAYG
                     DGRQDRIDGLTVAYDTFWFNLRPSNTEPLLRLNIEADSPRILEVQKAKLLGLIRS"
     gene            18735..19505
                     /locus_tag="PKM94_02485"
     CDS             18735..19505
                     /locus_tag="PKM94_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65532.1"
                     /translation="MVDTPIRLLVVDDDPLLRELLQDTLDTLGYRVTAAADGIEALER
                     LAQGSFDLVITDIKMPGMDGIALLKRVNRLYPDLPVLFITGVASPEVIGQASPVGFLA
                     KPFRISHIERLISDALHWRKERVEHPIRRVMVVDDDDLFREMLADTLTGHGFTACAVQ
                     NGPQALSELENGPVDAVITDIKMPGMDGIALMQAIKERHPELPVVLITAFMSEAELGP
                     ATPAARADGFLRKPFEIDRILELLDSLAPSPVDGRAGS"
     gene            19619..22030
                     /locus_tag="PKM94_02490"
     CDS             19619..22030
                     /locus_tag="PKM94_02490"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOD65533.1"
                     /translation="MTPRTRRMLGGAALVFAGLAVGFAIAVWATLDGTLPPCDGEIRL
                     AGLDGPIEITFDSMGVAQVWAETAHDGLFGLGWLHAADRLFQADFTRRVAQGWLSELL
                     GAATAAFDSERRRDGHARLARAALAKLSPENQARLQAYADGINAYRARGRRPLEYLLL
                     RTEAEEWRVYDCLTVLSFMTWYSDALQNRDHFFAELVREGRAGVLDSLPLGYPSGALP
                     PGCGERAEAAGRLNLGGGAEAFTPARSSNAWAVAPARSASGKAIFCSDPHLELTRLPQ
                     FWYAVGLHTRRPQLDAVGVTIPGLPFVVMGHNGQAAWAFTAGGVDLVDYYVERVNPGD
                     PEEYLERIDPARGADSLVWSRIEVVRDTVAIAGSDSGAVIQLRFTRHGAVDPEWSGRD
                     SLVVCRWAGLEADLDRAATAGWELMQTRTFAEFRGAVTAMGALNANWLYADAGGTIGY
                     QLGSPVPRRGEASPYAPLAGWAAEDDWKGYLSGDERPWCENPPEGWLANCNNRTAERP
                     DIPGSYAIDRIVRIEALLRSQERFTADDMRRFQLDRIDAYALRWKGLIDSVGAAIGDT
                     TGALAAVASWDGSADAASRAAGLVRVFLMRLGPAVFADELGEGSEGLGTAAVDQVFFG
                     GDSTWLDDRTTAGRIENRYEIAGRALREALDIARGRTWGEMQTLQMRHPMADAPVIGG
                     LIDLAFGPWPWGGTSGTLNSSFCRQPEPGRFETIVGPSWRFILDFADVEGALMVLPAG
                     NSGHPLSPHFFDFNESWRNGRYWNVPIGRDRVYRRAASLLRLAPGDTVQPGSERSSAL
                     IRSNS"
     gene            complement(21982..22713)
                     /locus_tag="PKM94_02495"
     CDS             complement(21982..22713)
                     /locus_tag="PKM94_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD65534.1"
                     /translation="MSESVDIEAIILAGGQGTRLYPHTAEIPKPLVTVGDRPIIEILI
                     HTLRRRGIRRLRLAVSHLAHLIEAALGDGRGLGVEITYSREPRPLSTIGPLTLMEDLP
                     EHFLVVNGDILTDLDFRRLYEGHRERGVVATVATCRRENRVDYGVIETDAQGRLAAFH
                     EKPARTVTVSAGVYVFSREILRYVPRGERFGFDDLMATLLARGEPVATYPIAGYWLDV
                     GRLEDYAVANQEFDRIKALLLSDPG"
     gene            complement(22722..23708)
                     /locus_tag="PKM94_02500"
     CDS             complement(22722..23708)
                     /locus_tag="PKM94_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD65535.1"
                     /translation="MTHRPQTVLVTGGAGYIGSVLVRVLLRAGLRVRTIDNLSWGGEA
                     LLGVLGDPSFEFVFGDIRKQSAVDAALRGVDSLVHLAAVVGDPACRRQPELAAAVNGE
                     ASELLLARARAAGVGRFVFASTCSNYGRAPDGAVCDEDAPLRPISLYAELKVAFERRL
                     LALEAPGLTTVCLRFATAYGLSGRPRFDLTVNEFTRDLFLRKPLEIYGEQFWRPYCHV
                     ADLAAACCLALAAPPEAIDRRAFNVGHTDENFRKADLVAMILAHLPDRAGLVTFVRRE
                     EDPRDYRVNCDRIRDTLGFIPRRRVADGIREIVAALTAGLITDPENPRYRNV"
     gene            complement(23719..25917)
                     /locus_tag="PKM94_02505"
     CDS             complement(23719..25917)
                     /locus_tag="PKM94_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65536.1"
                     /translation="MPRFLTATLLSALLLAALGCSESPQEKKQRLFDEVIAAANACDF
                     PAAQKSLQGLTDLEPTAPSARFASGYILERQLQHYDALGVYVALTNTFPSFTDAHAGA
                     WRLYTRLGLLPEALREAEALRELLPHDAEAAVILALALIDSNLPRRAHPFLDSAAAEG
                     ADPARVAMIRARAFIAADQPNAADSAYEAALVGAGSSPEVYLQAASYLETAGLIDSAV
                     TLSRKAVDLSRGDVPTLQQHFELAVRHNYFAEADRVLARLRGAKIPETVVSMLEAFRY
                     DAARRAMPAGEAIDRVIGLGGGQLSMRMYESAIKTKYSNEIVLLHNIQTVSTFLINEG
                     YDREFQEAVFYLLALQAADWLRGKEALVQWEQIRPMYWSRPHIAARHAWALFESGQFD
                     IFATEVEQIHRLHSSQPEWLAELGDVYADIILHQYDTAAALYERALELNPWHRPAFTG
                     WVGMLRRLDRFDEAAKLFDRYPHFPERFPALAMLEGICLAEAGQPERGLEVYLMNAPQ
                     NLGDEAMFQNFFEVLTFRAYREPMRRAAEWIMETGAEHPHILALAAQLRLELGDYPGA
                     IAAADAALKVLPDLLDAKAYRARALCGTGDVDGAIATLRANLDRDQFHVPSTYWLSRI
                     LAEQGREPAQAQDFARRALFDSNQGIREWLNLSYVYQVTGRYDLARGEAIKMTRSFAG
                     EPGPLFRLGMALYLENKPDEARLRLQEAIDRGLAGDDLAEARRVLSTLKS"
     gene            26125..26679
                     /locus_tag="PKM94_02510"
     CDS             26125..26679
                     /locus_tag="PKM94_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOD65537.1"
                     /translation="MARIQKPPMGRLFVSIIYSSMDALADGLRALERKFGRVQYETVE
                     IACSDRELYREEMGENLQRRLFSFERLFPRDALPEIKGVCHRVEPQFADVVGDFVFRT
                     VNIDPGIITPDQVATASHREFSHRLYLREGVFAQVELIWSHGQFHRLPWTKPDYCEGE
                     AIEFFQRVRESLEAAPEYEQPVSG"
     gene            27201..29207
                     /locus_tag="PKM94_02515"
     CDS             27201..29207
                     /locus_tag="PKM94_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65538.1"
                     /translation="MRNLVKGIVLAVAALLLLWGSASAMVEGVRVSTPDTCWYQRGFI
                     AFPIAIEWTTWTQHPPTAFPDPGPVDFPYRCNDAEYWVHPSHDVLVRPTVTIGYPGLT
                     YCNLPTDLPDSANPFGAPDETMCGFEMTVTFDEDVLQAVNVSNSELLTDDWGWGPVFY
                     EIDNARGIIHIASASADCEDITGLDDPTTLIWIGFYINGSPGENADLMVEEFTYNETD
                     PYYVFIDNEQYYQDVDEGIPGSEQVYLGNQSIGDFLVCEFLSASGQVLYMANGVPICG
                     ADVTLYYHPDTTLVIAPVIDNLTYETQCVIPCEDDCRGSYYIPGITGGYDYSLGAWHD
                     DEDAYPIAITAFDASLILRHIVGAFEFREPFHYIAADVSGSCEVSSMDASLILQYLVG
                     EFDYFPKKADEETNWLFVTDYFTKSDPYLCPEEQYWFEPMEDNHPGHNFWAVVLGDVS
                     GNWGVGMTIPPAKESGSPVAVRLIGEADGLATYELSVPGEPAFAFDFAVEASTRLVSV
                     SPGEDWITQIAEQAESRHVVAAGVSATTVLGTVEVAADRETLQLRRLVVNETNYGTVT
                     VALKGEPTAPRSFALHEAYPNPFNPATTIGFALPQACQVTLTVYDILGREVKILASGA
                     MDAGEHNVTWDGTDRSGRTVATGVYLYRLTADGFTQCRKMMLLK"
     gene            29265..30011
                     /locus_tag="PKM94_02520"
     CDS             29265..30011
                     /locus_tag="PKM94_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65539.1"
                     /translation="MHKRLTFLPVLLLAAGVSGVQGEVNGTLVCDELADTLLGVSAIL
                     WSISDFAEPVYSLEIEACFDPACVRIRDVDYTASLVAGWGEQFVDRLDADAGTFALAV
                     AGTLPLAADSGTVFRLTLAKAAGLPPPVPVGRVTRFRINETSLVSTPPGGGETPRGYD
                     LAQNYPNPFNPGTTIRFALGRAEAPVVSIYNQLGQVVRRDHLGPLPPGPHVYVWDGTD
                     ERGRPVAGGVYLYRLETANFTRSKKMILVK"
     gene            30044..30853
                     /locus_tag="PKM94_02525"
     CDS             30044..30853
                     /locus_tag="PKM94_02525"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65540.1"
                     /translation="MRRTQNTLAIILAAAIGLAGAPAWGMSLPFQWATNPEPVTSLPP
                     GDTLRIGLYVDSLMKAPIGGWLQGVQVNLAYDEDRLHWERMEWTGTLLQNWIRFVGCD
                     SAFGEIAVAAATAKDSIVENGFLLFLVFTNCSSSNVILQPGALPGLLEVETVILNEEH
                     VDPFPVVGVSEPEPPLPAGYALAQNYPNPFNPATTIAFRLAEAGDVAVAVYNAAGQLV
                     RLLRCGRLGPGNHAVMWDGRAEDGAPAASGCYLYRLEGDVVSPARKMLLVK"
     gene            31141..32403
                     /gene="murA"
                     /locus_tag="PKM94_02530"
     CDS             31141..32403
                     /gene="murA"
                     /locus_tag="PKM94_02530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOD65541.1"
                     /translation="MDKLVINGSRPLTGQIRVEGSKNAALPIIFGALLIDKGETVIRN
                     VPPLRDIFTTIKVMEHLGAKITYDPKAQVMTVNAEQITENTAPYELMRQMRASFLVLG
                     PILARLGRARVSLPGGCVLGARPVDFHIKGFRALGAKVTEEAGYVIAEAKPLAGGSVY
                     FDRPSHTGTENLLFGTVFAKRRTFIANAACDPEVVDVANFLNKAGARITGAGTPNVVI
                     EPVRRLKAVEYTVSGDRLVAGTFLMAGAITGGRVKVAGCDPEHLRVVLHKLGEMGCTS
                     EATRTTVTVAGPKRLQPVRVTTFPYPGFPTDLQACIMAMACIAEGTSHIRETVFEDRF
                     SHTMEMRRLGADISVSSDEAVINGVSALRGATVMASDIRAGAGIVLAALAAAGRSEIL
                     RVYHIDRGYNRIEEKLGALGADITREAE"
     gene            32494..35886
                     /locus_tag="PKM94_02535"
     CDS             32494..35886
                     /locus_tag="PKM94_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOD65542.1"
                     /translation="MRKSHILAPLLALAAALVVSGTAPAQETYFGKNKVRYKNFDWEY
                     IQTRHFDIHFYEGAYPTAKFAAAVLESAYVEITQELGYHLQEPVPIFVYNSHNDFQQT
                     NIISSLIPEGVGGFTEAFKNRIVIPFTGSYEDFRHVLHHELTHAVIYDLLFGKSLSSL
                     ISRNRLFNLPLWFAEGYAEYSSRHGWDYWADMVVRDATINGYLAPPDYVGGYLAYKQG
                     QAMIKYIADKYGEDKLGEILRKGKVYLTMDRALKEAIGIDQEKLWKEFSLEMKRHYWP
                     EIASRKNADEIGRQLTKARKDGSYMNEEPAYSPEGDKIAIFTDRSDYTEIVLISAADG
                     RRVKTLVKAERSGDLESLHSYVSGISWSPDGRSIAFVAKSGGRDAIFLYDVRSGDVKV
                     KRVLPYYNVVSPAWSPDGSRIAFTALKDYKRDLFVYTIATDEVEQITDDRFDDNSPSW
                     VPGANALMFSSDRPHPQSPEARAEVNPYASPGAFMPGDFVYGYYNIFRVDLGDRTVAP
                     VDVGPGQNKTPEVSSDGSRLAFISNRNGIDNIYVQVLATGQCFAVTDILSGVKSLSWS
                     PDNEKIAFEAFHEGAFDIFVLEDLQPSGDHGLLAATDFVKGAYNRAVKQRAGQALASH
                     QEAVDSAAVARPNTAAADTATAFAEGERRGDSLALGAAPDSTQAADTAAVPTGGAPSG
                     ATAAAPPDTAAADTARADSAGVVKKTGVYGGEYVFVSEGRRDPLDSVLEEVSPDSIRA
                     LREGQVEPEKFDERPAAASDGDYAVKKYKVKFTPDYVGGGVAYDTYFGLRGQTYFIFS
                     DYLGNHQFYVATELVNTIDQSFIQAYYFNSVHRTSFGGGVFHTKNFYLDANDYLFSDR
                     FYGIQLFTTYPFSTFARMQLSGAQIFIDREYHDQYDLRPNRNSKVTTGELAYVADNIV
                     WGITGPVNGTRARLAVEAGVNLFDANDIAFQAASFDYRRYWHFRKTYSMALRLTGGAS
                     FGKTPKLYFLGGTTNWIGNRTVDAKVYDVENLYFLDVVTPLRGVEYYELSGNRYGLVN
                     WEFRFPLIDYFVMRFPLGLALTNIQGVIFTDFGAAWTDSDFKFGTSENGHRRLADPKA
                     GFGFGMRANLGFVVLRYDLAWSTDFAEVSARSRSYLSLGADF"
     gene            complement(36088..38640)
                     /locus_tag="PKM94_02540"
     CDS             complement(36088..38640)
                     /locus_tag="PKM94_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65543.1"
                     /translation="MPPKTKRTRKHTELTARQLDYSVNFRPQGVKSSDDVKPAVGVIG
                     QDRAIEAIRTGLNMTSPGYNIFVTGSVGTGRTDATLHLLEQFTRAKEPKLPDICYVNN
                     FKNEDCPRVLTFRAGQGSRFRKDMSYLIQSLRKVVPKVFMSEDYKDRQSRIIREYENR
                     QKELIHAFEERLSNVGFVMVQIQSGLGTRNEIQPLIEDNPVSLEQLERQARDGKFPLS
                     RLDQLRRTWDSLRREFDTITIEAKKLTGKMEDALEKLNHSMVAPLIADKVSLLKRRYP
                     GAKVVEYLDEVAEALQSDLERFRESQARRGEDEAPPYRKREPFEEFSVNLILDNAGAR
                     RVPVVFEKSPSYKNLFGSLERVVDRFGYWRTDFTRIFGGSLLRAAGGYLVLDALDALV
                     EPGVWTHLKRSLRNGEIEIAAYDPFYMMAGSGIKPEPIPIDVKVVLIGEPRVHQLLWH
                     LDEDFKKIFKIKAEFDTEVTADRSNFKRYFEFLRKVVDEESLPPFDLSGMQAVVEYGM
                     RLSGRRDKLSSRFSAISDIIREAAYCARQRGAAAANRDDVHCAITLKRRRVNLTEEKI
                     QEMFDQNVLMVDTTGAAVGRINGLSVYHIGDYSFGRPSRITASTSLGRSGIINIEREA
                     DLSGPIHNKGVLVMSGYLRQLFAQDKPLAVSASITFEQAYSGVDGDSASSAEVYAILS
                     SLGGLPIRQGIAVTGSVNQQGEIQPIGGVNQKVEGWFDVCAARGLTGDQGVIIPHQNV
                     NDLLIRPDVIAAVKARRFHIWPIRTIAEAIEILTGLPAGKRGANGRFPKGTVFGIVDE
                     KLMEMALTLQNFGRDDESSNRTDSAGKKRAGGAKESPRKRGRRRAAHPRRRT"
     gene            39112..39183
                     /locus_tag="PKM94_02545"
     tRNA            39112..39183
                     /locus_tag="PKM94_02545"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39145..39147,aa:Glu,seq:ctc)
     gene            complement(39276..40394)
                     /locus_tag="PKM94_02550"
     CDS             complement(39276..40394)
                     /locus_tag="PKM94_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65544.1"
                     /translation="MAVRIEPYTDRHTFEVTQFNRRLRHGGVRSQFPENPVPAWLPPR
                     AGSDLRQECYVAVDDGGAVRGGYILKHQPFRAGGEVVAVGNYQLPLSEGMIDRTYAAV
                     GPQLLADALRRRPVLYALGLGGFDRPLPQMLAATGWQLKAVPFHFRVCHPQSFLRNLS
                     LLRERPAWRILSDVAAATGVGSIGILSLQASRRKKRARLGRTAAEIVPAFDHWTNGLW
                     ESGAGHYSFAAVRRAPELNLLYPAEDRRIHRLQVALDGAPVGWAVVLHTLMKNHNYFG
                     NMHVGTIADCFAAPEQADAVIAAATDYLEERDVDLIVSNQMHPWWCSALANGGFLSGP
                     SNFILATAPALTRRLAAADPAWERLHINRGDGDGPIDL"
     gene            complement(40385..40528)
                     /locus_tag="PKM94_02555"
     CDS             complement(40385..40528)
                     /locus_tag="PKM94_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65545.1"
                     /translation="MTVHDSALEAVYAALDDLDRRRSADQRLPRRADAAQGAGRCGLY
                     RWL"
     gene            40753..41628
                     /locus_tag="PKM94_02560"
     CDS             40753..41628
                     /locus_tag="PKM94_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65546.1"
                     /translation="MRLQVPLRGAALIGAAVLADWLPAAAQESAASTSVFRADRINAL
                     IFVVLFSTAILLYTRWARQGKPLFLRKIAGLDALEEAVGRATEMGKPVLFIPGILELD
                     DIQTIASISILGRVAKITAQYETPLYVPVRYPLVLAAGQEVVEQAYREAGRPDAYDRD
                     MVRYVAGEQFAFTANVNGYMVREHPATNIYMGGFFAESLILAETGNAAGSIQIAGTAE
                     ASQLPFFVAACDYTLMGEELYAASAYLSHEPVMLGGLKGQDFVKLIIIIVIVVGAVLI
                     ALGFGDTFVGWFDSV"
     gene            41650..42309
                     /locus_tag="PKM94_02565"
     CDS             41650..42309
                     /locus_tag="PKM94_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65547.1"
                     /translation="MRTTFPIFVAFAVGLLYVVSFFFAESSPIGRVSETLLVWVSIIG
                     GFTLLLGVVSIVRVNWTAVARRRPGWIYRLCTLVSVFVMALPALLPASWSPLFGRAEG
                     SVYDWLFLYIDSPMMATMFAMLAFYIASAAYRAFRARNIEATILLLTAVVVMLWRVPM
                     GEAFLNLLPGDIPGWLNTYVMRGVNVAVQRGILIGAALGAASMSLRIILGIERTYMGK
                     G"
     gene            42314..43150
                     /locus_tag="PKM94_02570"
     CDS             42314..43150
                     /locus_tag="PKM94_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65548.1"
                     /translation="MAWWQAIDKLDRRWTYLLVAIAVIIPFILPAKFPVSITPEAKQL
                     YNYVEELPNGSVVMLVFDYYPSTIAECEPMTRTALHHLFRKDCKVVTLSNIPLGGPTM
                     AESVTREMARLYGKVYGVDFVNLGYKADYVAVLRGMGQSIKGIFPADNSGTPLDSLPL
                     MRKVKNYRDVAFIFEVADNATADYWASIVNAQFGVPMGCGTTAVSAPRYYAFVSSGQF
                     VGLLGGMKGAAEYEMLVGHPGMAYRGMDAQSLVHLVIIGLVVVGNVGFFAGRASRRRG
                     AR"
     gene            43147..44163
                     /locus_tag="PKM94_02575"
     CDS             43147..44163
                     /locus_tag="PKM94_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65549.1"
                     /translation="MSGMHVFEIWIIAFFTLALFSFLYKDNPIYRFAEHIFAGLSAGY
                     YVGLIWHTVILQQLWNPILGGRWLLVIPGLLGALMFARFSSKKAWISRTPLAFVMAVT
                     AGIFLISQLHGLVLPQVQDTMLPKTEAVLVDSTAYEEQADRLVITDSLAAPGAPATAG
                     SVTVSKPEVARTGTDSVVISATFRDPAQPPVERVAFTLKLRLPDDKTQKALVVNATTR
                     TPGVTIAAGGGGVYTASYVYRPDSTRAPGPYDISFQAYRVDHEVMLTLLVLLGVITTL
                     IYFYFSKEHTGALGVAARIGIWFIMIGFGAHFGYTVMGRVSLLIGRVQFLLEDWIGTL
                     TSLF"
     gene            44199..45281
                     /locus_tag="PKM94_02580"
     CDS             44199..45281
                     /locus_tag="PKM94_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65550.1"
                     /translation="MFSRAGAGVLAAAVIFLACSGEYDGAKPAYDTARNPGQFPPPAT
                     ALLDDLEAGRLRGYDETTERFADLYTAHPALLGDPGWSGVIDRLGLRLTWRGDQLVRE
                     GLLQYAAAADLYRLAAQTRPKDSLLSERAELFAAWQEAVGRGAVDPVCLAAPGRGGAA
                     EKLEALTGFALAGARQFRFAEQYLAAALFDRATLDSLRRAEAAGPTAAERALRAALGA
                     GPSPADPPLASFRQPAVDLIAVRLTPAAADSFCLEAYFLPRAKVSADLDITCRLETPD
                     PVLSAEQQPRFPYDFRPVRPSSMWAPGRIAVGLYRGTFPHPVSAVLIGLHEMREGRTV
                     ALPLGDRSTLCRVPVGASGGTPDPPL"
     gene            45353..46339
                     /locus_tag="PKM94_02585"
     CDS             45353..46339
                     /locus_tag="PKM94_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD65551.1"
                     /translation="MSKRIRVIVPVAGAGTRLQPHTLTLPKALLPVGGKPALAHVLEP
                     LVPLEPEEVVFVIGHLGDQIIDYVRENYRIKARFAAQEKLLGLGYALHLALREMADGP
                     TLVILGDTVVECDYGAFLGAGEYVLGLRSVDNPHRFGVAEVDGGRIVGLEEKPADPRS
                     NLALIGLYYFSDTAQLRKELEYLVRTGKTTGGEIQLTDALHAMIAAGVRFAPYEVARW
                     YDCGKMETLISSNREILKQRATPAAPAGSVFVPPVYAAPTATIINSIIGPNVSIGEAA
                     RIERSVVSNAIIGERSQIVNAVLDHTLIGRDVFLEGTPRRMNVGDKSQVGDV"
     gene            46390..47553
                     /gene="metK"
                     /locus_tag="PKM94_02590"
     CDS             46390..47553
                     /gene="metK"
                     /locus_tag="PKM94_02590"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOD65552.1"
                     /translation="MPGGNFLFTSESVTEGHPDKMCDQISDGVLDEVLRRDPHGRVAC
                     ETFVTVGLVIVGGEITTDAWVDIERLVRGVVKRIGYVDPTLGFSYDSCAILNAIGQQS
                     PDIKQGVDTGGAGDQGLMCGYASRETAELMPMPIQFAHKLCRRLAEVRKKAILPYLGP
                     DGKAQVTIEYEEGRPRRCEAIVVSTQHRESILDATGKKITEQARAEIIQNVVAPVIPA
                     DMIDARTKFYVNPTGRFVIGGPRSDTGMTGRKIIVDTYGGMAAHGGGAFSGKDPTKVD
                     RSASYMARYIAKNVVASGIAEKCTLQVAYAIGVAEPVSLTVFADGTAKIPERRIAELI
                     RKHFDLTPRGIIEKLNLLRPIYSATAAYGHFGREESEFTWERTDMAAALAKDA"
     gene            47557..48816
                     /gene="ahcY"
                     /locus_tag="PKM94_02595"
     CDS             47557..48816
                     /gene="ahcY"
                     /locus_tag="PKM94_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOD65553.1"
                     /translation="MGKRTDIHNPRLAGPGRDRIEWAEQRMPVLRLIRERFRKEQPLK
                     GLRLACCLHVTTETANLVRTLQAGGATVALCASNPLSTQDDTAASLVKDFGIAVYAIH
                     GEDTKTYYRHIHQALDGRPHITMDDGADLVSTLHSERQELIGNILAGTEETTTGVIRL
                     RAMAQEKRLLFPVIAVNDSRTKHFFDNRYGTGQSTIDGVLRATNMLIAGSTVVIAGYG
                     WCGRGLAMRAKGMGAQVIVTEVDALKALEATMDGYTVLPMAQAAPKGDLFITVTGDLH
                     VIRLEHFRRMKDGAIVCNSGHFNVELDLAALAKAARRKRRVRPNVDEYVLDRRRIYVL
                     GEGRLINLAAAEGHPAMVMDMSFANQALAAEYVVHNHALLDRCVYTVPEAIDRQIAAL
                     KLKSMGIALDRLTPEQRKYLASWDMGT"
     gene            49093..49674
                     /locus_tag="PKM94_02600"
     CDS             49093..49674
                     /locus_tag="PKM94_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HOD65554.1"
                     /translation="MRVRTLVLGLVAAAVLPGLVLAQSDAPSQSEQGMPPMGPPEEMK
                     ECAWLVGDWTADMKMRMAADSPWVATRGSAHYETVLDGGALLMTFEIPPSAEMPMLFR
                     GMGLQTWDRETNQWQMTWTDNMTCRTTIYTGPRTEGQTVMTGEDLWNGMKMLSRITAS
                     DETPTGFDWLMETSLDGGKSWYASGQAKYTRVK"
     gene            49911..50252
                     /locus_tag="PKM94_02605"
     CDS             49911..50252
                     /locus_tag="PKM94_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD65555.1"
                     /translation="MRFRDHLDGRIAVFELSGKIMGGRDSTLFQGRVNEYVNINRNRI
                     LIDMAGVEWTNSRGLGMLIAALRLVQGSGGRLALTNIERVRSLLAVSRLATVFEHFDS
                     RADALEALRSD"
     gene            50470..>51640
                     /locus_tag="PKM94_02610"
     CDS             50470..>51640
                     /locus_tag="PKM94_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65556.1"
                     /translation="MPIPRCAVCLAAALLITLTMASCGDDDGSTNSGFVAPGVHWTIR
                     STGTERPLFDMAKGDTLWVAVGGAGTVLTSTDGQLWTTQPTDLTIDLYGIASNGQRFV
                     AVGGQGRIVTSTDGLTWTTAESTFTARFFFDVAWSGEAFVAVGQGGLVAASADGLTWE
                     IYSSAVANTLLGVRWMGSRFLAVGEKIILRLDPPELDGLDSLTAADVLARPPYVLYDA
                     AVSDSGMLAVGQYGTILKAVNDTTWAVRVSGLNAYLYAIEWVGDRFVAAGENGIILTS
                     ADAARWELIATLDDDIRGLYWDGAQTLAVGVAGTILRSVDGHEWTAVPSGPNAILSAV
                     AANESLYVAVGAGGAALVSRDAARWTLWPSGTTEPLGAVATDSTTFVAVGAAGVIA"
BASE COUNT         9324 a        15611 c        17138 g         9567 t
ORIGIN      
        1 ctggaatcga tgggactgaa gacgttcggt ttcgccgggg gccgcaccga tgtctgggag
       61 cccgaggagg atatctactg gggccccgag gctgagtggc gcggcagcac gcgctattcg
      121 ggggaccggg agctggagaa tccgctcgcc gcggtcgaga tgggtctgat ctacgtgaac
      181 ccggaaggtc cgaacggcaa cccggacccg gtggcttccg ggcgcgacgt ccgggagacc
      241 tttgcgcgca tggccatgaa cgatgaggag acagtcgcgc tcgtcgccgg ggggcacacc
      301 ttcggcaagt gccacggcgc gggcccggcc tcgcacgtgg ggcccgagcc cgaggccgcc
      361 ggcctcgcgg agcagggcct cggctggaag agcagtttcc gcagcggcaa aggcggcgac
      421 acgatcacca gcggcatcga aggggcgtgg aaaccgcacc cgacgaaatg ggacatgggc
      481 tatctcaagg tgctgttcaa gtacgactgg gagttggtga agagtccggc cggcgcgaac
      541 cagtggctgg ccaaggacgt cgcggaagag gacatggtcg tcgatgcgca cgacccctcc
      601 aaaaagcacc ggcccatgat gaccaccgcc gacttgtccc tgaagttcga cccgatctac
      661 gagccgatcg cgcggagtta tctcgagaat cccaggaagt ttgcggatgc ctttgcgcgg
      721 gcgtggttca aactgaccca ccgcgacatg ggtccccgct cccggtacct cggcccggag
      781 gtccccgcgg aagagctcat ctggcaggat cccatcccgg ctgtcaatca caggctgatc
      841 accaagcggg acgccgccgc tctcagggcc gagattctgg catccggttt gtcggtggcc
      901 gagctcgtat cgaccgcctg ggcatcggct tccacgttcc gcggttccga caaacgcggc
      961 ggggccaacg gcgcgcgcat tcgcctcgca ccgcagaagg actgggaggt caaccagccg
     1021 gccctgctcg ccaaggtcct tcggactctc gagcgcatcc aaaaagcgtt caacaagtcg
     1081 cagtccgacg gcaagaaggt gtcgctggcc gatctgatcg tcctgggcgg ctgtgcgggc
     1141 gtcgaactgg cggcgaaaaa ggccgggttc aaggtcacgg ttcctttcac gccgggacgc
     1201 atggacgcct cgcagaagca gaccgacgcg gcctccttcg cagtcctcga gcccaaggcc
     1261 gacggtttcc gcaattacgt gaaagcccgg tacagcgtgc gggtcgagga actgctggtc
     1321 gacagggcgc agttgctcac gctgaccgca cccgagatga ccgttctcct cggcggcctg
     1381 cgcgtcctgg gcgccaactt cgggggatcc ccccacggcg tgttcaccgc gcgcccggaa
     1441 actctcacca acgacttctt tgtgaacctg ctcgacctga acaccgtgtg gaaaccgacc
     1501 gcgccggacg aggagctctt tgaggggcgc gaccgggcga cgggcgcgct caagtggacg
     1561 gggacccgcg tcgatctcgt cttcggctcg aactcccagc tccgggctct cgcggaggtc
     1621 tacgcctgtt cggattccct ggagaagttc gtgcaggact ttgtcgcggc gtgggacaag
     1681 gtgatgaacc ttgaccgctt cgacctcgcc taaacggagc gagcggatcg gacagaaagg
     1741 aggttggctc ggtcaacctc ctttttcttg gccggactct tgcatgattg gccgatcagt
     1801 cggatgtggg gcaatccgcg aaaagcgcga tcataaaccg gccacgcgga caccctcgcc
     1861 gccgaatcgc gcgggcccgg caggcttcct ccgggcgcgg gtgacgcggc gatcgggacg
     1921 cctgtccgcc gtgattatgg ttctgcgcgt tatttctctt ggcccgaacc cggcttctca
     1981 ctactgtcgg caccgatcca tttgcttgcc gcggcgcgcc acgaagaact cataggcata
     2041 gtagtcggag taccggcggt gcatgtccgg ctcctgcgcg agctggtcga ggaccgccag
     2101 cgcctcgggc tcggccgcgt aggcggcgcg caggtgttcg atgcggcgtt ccatcggcgt
     2161 gtagaagtct tcccaccagg cctcatcggg cagagtgaaa tgtcccagga gcgagaaccc
     2221 ggagccggcg atgatcgcca gaatatcggg gacggcgccc atggccgggt agtcgacgtc
     2281 gaagctctct ttgacctccg gcggagggtt cgccttgcgc cagacggcat cggtgaacgc
     2341 gaggtaaccg cctgagcgga ggagcccgtg gcagacacgc agcgcgtggg cgatgccgat
     2401 gttgtagagc gcgccctcgg accagaccag gtcaaagccg gccgggggaa ggccgggatc
     2461 ggccatgtcg ccgaccaccg ggaatactcg tgcggagagc cccagccggg acaccgcggc
     2521 ggcgagccgc tcgatgctcg gcgcgtggcg gtcgagagcg acgatcgggc cggaggtcag
     2581 ttcggcgagg tggagcgttt gccctccgac accgcacccg aggtcgagca cggcggggga
     2641 cggcggcagg tctcggcaca gggccagcgc ccgggcggca caggcgcggt tgcccggccc
     2701 ctgccgggga agcgactcga agacctcgaa gaatatctcc cagaaacgcg gcgtcggttc
     2761 gcccatgtca tgcctcgtct tgcctcagga ttctttcacc tgcggtttct gtctcctatc
     2821 gcggttggct acgccctatg gtaatgaatc ttccccggct ttagcaaacc tcatcgccgc
     2881 ctcacacaca accggctgaa ggcaggtccg atggccgcct tgatgcatcc gctgcgcgca
     2941 ggcgacgcca tcagcacttg accaggctgc gggaaccggc cgagcggcag gcgccggccg
     3001 ggaagggccg gaggcgccgg ccgatgaggt gcggcgcgaa gcgaatccgg atgagaccga
     3061 cagagggctg gcagcagccg ggggatctta gaaagcgagc cgggtgagcc gccgcggggg
     3121 gggaccggtc ttgcacggcc cgtgactcat gccggggaac atgaagggcc gggtctacgt
     3181 atgcttccac atggcaaggc gccgggcagt cttctggaaa ctcgctgcgt gcgattggaa
     3241 cgtcggccgt ttcatccgag ggagcagtat gagagaagcg cgcttctcgc aaccgtgcat
     3301 cctcgctgca actgcgataa tggcggcgct cctcgccgcc gatctggacg cgtgcaccgc
     3361 ttttatcgtc aggcaggggc accaggtgct ggtggccaac aatgaagatt ggaccgagac
     3421 gaatgtccgc gtgtggtttg tccccggatc accagggcgg cacggcgccg tctacttcgg
     3481 ttttgcagat actcccggtg gcgaatcccg agaagggggg atgaatgacg aggggctgtt
     3541 tttcgacggt ttcggtgctc ccgccatagg ggacagcaac cctctgaaca agccgctcta
     3601 tcaggattcg gtcggttttc ccgacaaggc cctggcggag tgtgggagcg ttgaggaggt
     3661 gacggcgctc gcgtcgcaat tccacctgcc gctccatcac gccatgtgga tgttcgggga
     3721 ccggtacggc aattccataa tcattgaagg ggagaaggca gtcgcgggcg atggccgcta
     3781 tcagatctgc accaatttcc gccaatccga gctgcaaggc gacatcgctc cctgctggcg
     3841 ataccgaacg gcggacagcc tgctggaaaa catgccggcg ttgtcggtgg cgtactgccg
     3901 cgacatactc gacgcagtcc atctgaagat tacccagtat tcctacattc ttgatccgaa
     3961 ctcaggacag atctacttct accactttca cgattactcc caggttgtgc aggtgaatct
     4021 ggttgaggaa cttaccgcgg ggccgcacta tatcgacatg ttgccccgct tccccaggaa
     4081 gctgggtgtt acgctgttgg ctgagcaggg gaaggcctgt tatctgagtt tcagcatggt
     4141 gtttttcgct gcctcgctga ttgtgtgggc gggacgcgtt ctgttccgca gaatcgtgcg
     4201 cgtgaagccg acaggctccc tggcgcaacg ctcgcccgga gctgccgctg ggctgatcgc
     4261 gggcgtcctg tgcgccgtca ttggggccta cctcgtggtt ctccttggat acccggagat
     4321 gattccatac tcacaggaaa acgcgagcgg ccactccccg ttcctgcggt ttgtcctgct
     4381 gggagcgccc tggctgggcg cggcgctgct tggtgcatcg atctactatg gttgggccgt
     4441 gtggaagacg cgagcgtggc gcctggtcgg acgggcgcac tatactctcc tcgcgctgat
     4501 cgcctcggct gatgtcgtga tctgggttgc ctgggggatc atcccgtgat cgccacagac
     4561 tcttgtgggc ctccagtcag tagtcccgtt ccactacgcc gggttcaacg gaggtcgtgg
     4621 ggaagcaata ggccgaactg caacctgggc gccctgaccc gcgtaatctc gcttgagaca
     4681 ccagcaggaa ggagtaggtt atgcgagttg ttacggtttg cgccgtagct cttctttccg
     4741 ccgtttcatt cgccggtgtc gacggccggc aggccaatta ccttgtgcca aatggccccg
     4801 atctcggaca tgaaacgccg agcaggaccg ctcagtgcgg gtctgttgcg aaactcccga
     4861 tgcgggtcga gggacggtca tgtacatcgt tttgcctcga caacaagggt gaggccgtct
     4921 tcgggtcaaa ttacgacaac gacatccccg aggggatggt cttcatcaac aagcggggag
     4981 tctccaaatc cggtctcatg cccggcacca ccgggcaggt tgccacgtgg acatcgaagt
     5041 atgccagcgt cacattcacg gtggtcggcc accagttggc ctgggccggg atgaacgaac
     5101 agggtttggt gatgagcacc atggctctcg gcgaaacgca gaacccggag ccggacgaac
     5161 gcgcgcccct gcactccccg cactggatgc agtacctgct ggacaactgc agctccgtga
     5221 acgagatcct tgcggccgac tctcaggtca gaatattcga gacggtcgat cattacctgg
     5281 tttctgatgc ggccggcgac tgcgcggtga tcgagttcct cgatggcaag acggtcatgc
     5341 acaccggcca ggggcttccc gcggcagtcc tgaccaacag cacgtacgca gagtcggcgg
     5401 ccttgttgga cggctccggg ggggaatccg gcaagcggcg cgatccccag cccattaacg
     5461 gttcgctccg ccgcttcggg atcgccgcag aaagggtgca agcgtttgag ccggccggtg
     5521 cggaggacgc agtgacatat gcgttcgaca ttctcgacaa ggtgggtgga gacgcgacgc
     5581 tgtggagcat cgtcttcgac acgcatgacc tgacagtcta cttccgcacc aagtcgcacc
     5641 gggcgatcag gtacctctcg ctgaacgatt tcgacctctc gtgtcaggcg ccggacctga
     5701 tgctcgatat ccacgagaag ctggagggac acattgccga ggcgatgacg gtcttttcct
     5761 ccgatcggaa tttcgcgttc ctcaagaacg ccatcgcacg acgggagatc ccgatgagcg
     5821 acgagcagct gcaggcgatg cgcgcgattc tcgacagtta cgaatgcgag cagtaagcgg
     5881 ggcgccggga atcccagaag gggcgggcca cgcgggcgcg ggtttcatat cgcggcagca
     5941 gcagcggtgc cgcagcggca catcagtcaa cgcggccgcc ggccgacgcg atccgcaacc
     6001 gggtgatcga gaccgcagcg tgaccccgga gaccaggcaa tttcgacatc agagtcgcag
     6061 ttgcccagga aagtttcaag cttgacacgc tggagaaact gtgattcatt tccgatagcc
     6121 tgtgcaccgt accggccctc agtcgggatc tgtgcggtta accgggaagg aggtggttcg
     6181 agtcatgcgt atgaatcttg cgcggagtca tgctgtgagt gcgattcgga tcaccctcgc
     6241 cggcctcggt taggtgcaac cccgattctt cccccatgac tccgggttcc tgaacacggc
     6301 acaaaggagt catgaatgat cagccttact aatgaagacg gaatcacctg cgccagcctc
     6361 atggtcctgc aagaggatcg cgaggcctgg gcgatcgtcg tttcctggaa gaagggagtc
     6421 gacggcctcc cggaccgacg cgccttccat gaagcgctcg agtcaagcat cggggagtgc
     6481 gtgaggatgg gcgcgctcta tctcgacagc cgcgtgatta ctgcaagcac aggcgtcgat
     6541 gacgcgctga catcctcgcg cgctgcgctt caccgcgatt tcctctccgc ccgcggtttc
     6601 gtgagaggcg aggatcgcgt tgagtaccaa atggatctcg ccgttgcgct cagtatgctt
     6661 gaggcggacg agatcgaacc tagtcttgtc tgggaatgcg tcaacacgga atgcgcgtcg
     6721 gctctcgcgc gggcggccga tttgttctct caggcaagtg agggcgaccc tgcatcgaat
     6781 cctggcgaag atgcagtagg atttgtgaaa gccctgatcg aggagaaaga aacggtccaa
     6841 gcccccgaac gcctccagat cgggatgtgc gggaatgatc ccgccgcagt tctcgcgctc
     6901 aaggtctatc ccagcgacgg atggtcgacg atctactacc ttggcgtgct gccggttttc
     6961 cgcgggcgcg gattcggtgc cgccgccatg cttcaggcgt tacattctct gaaggccatg
     7021 ggcgggagga tctatcatga tggaaccgca tcggggaacg caggcgcgct ggcgctcttt
     7081 gcgcgcctcg gccagccccc attccgggtc atggagacat ggagattaag acgataggcc
     7141 gaggttcgat cgatcgtcat ggaagccggg cgccattctc aggaagtggc gtcccgtaaa
     7201 cacctgagcc gacgccttgc agcggctgat taggaatggt gcggccccga cctgactctc
     7261 cccggacctt ggtggcattt cattcgcaat gcggccgcac gatgccttca tcggcggaac
     7321 gcattggaac acaaccaaat gcaacgacgt gacatttgtc tggagacgat ttggcttgac
     7381 atcgatgcag cactcagtga gtttagtcga gaggcagtga cgatccggtt tgcaggctga
     7441 gtactcggat caacaactcg cctatctcac cttctgtgga ccagatcgac aactgatcct
     7501 cgtattctga cataaggagg atcaatggta cgtcccaagc tactttccaa tgcccagaag
     7561 atcatgattt gtctggttat cgtgcaggtc acgacgtcgg gagtcctcgc ttttgatggc
     7621 cagcgcaatg ggttcgtcct gggcgcaggt gtcggcctgt cagccatatc aacgctgtct
     7681 tcatcttaca tggggcacag cttcagcggg agcggtacgg gagtgggcat gaacgcgatc
     7741 atgggatatg gcataagcaa acatgatgtt cttgcggtcg gaatgaactg ggcggggtac
     7801 agaagcgatt acaccggcga gaacgccgat atcgcccagg gaatcgttgc cctcacatgg
     7861 acaaggtact tcggcaaacc gggcaagtca tatctagtac tggcggggcc gggtgcatac
     7921 ttcgcccgct cgttctcggg ggtcgatatc tcgatctgct gggactgccc tttcgaagac
     7981 atgcctaccg cgagaatgcc ggaagattgg gggtttggtc tgatcggcgg tgtgggtcgt
     8041 gaattgagta ctcaattcca acttgtagtg tacggcgcaa tcggttttcc ttcaggtaac
     8101 gtctacgtgc ctgatggcag ccaggctgcg aagacagtca cggctgcgca tgtcaacatt
     8161 ctcctgatgt ggatgagttt ctgacaaata ggagaggcac ctccaatgag ggaactccac
     8221 tctggcgagc cgtcaaattg tgcgaagcat ctgcttgaat tacggcgcag tctattacct
     8281 ggcgactacc tacataatgg cggccccggt ctcgcgctcc tcaaccctct gtgcaattcg
     8341 gtttcgatgt gcagcctgac tacgcgattc ttgacggata gtaccaaagg gccaaagaaa
     8401 acgaaaaggg ggcgaggaga gtaacctcat tcatcacgtc ggaaaacgta agtcgcccat
     8461 cccacagtat ctctgcccca cttgaggtac atgttcctgc tcgacgtaac ccggttcatg
     8521 agttcggtga tgtccggatc gtcggggttc tgacgggcgt attcatcggc cgccaaccac
     8581 tgcaggccct cgtattgatc ccagtcattc tggtcgctga caatggtata aacgaatcgg
     8641 agcccgtgtt tctctccagc ctccacgttg tcggcatgcg tcccgaagct ctccttttga
     8701 aggccggacg catccagata ctgcggtgat ggatcatgga gccagaaggg ctcccctacg
     8761 accaccaggc cgcccggggc agcccagctc ttcaatgttc tcagcgtttc actgtggtct
     8821 ttgaacaccc agctggcccc aatgcaggag accaaatgca gactcgcagg ctgttccggc
     8881 ctgaactcgg cgccatccat ctgcgtgaat gtgacgttgg gggtggacag tctatcggcg
     8941 gctcttttct tacaatcttc aacgcagtaa ggggaaatat ctactccgag tcctctgact
     9001 tcatatttct cagccaatcg aatcagaaac tctcccttgc cgcaggcgat atcgaccact
     9061 ccggcccctc tgggaaggtc cagaagtccg acgaggtggg ctagtttggc ctcgcttgtc
     9121 ggattgcaga ccaagtgctt ccggtgcgtg atattgtaga acttccaggt atccacagtc
     9181 actccacatt tgtatcgata tagcaatcac aacaggagga accattttgt caactgggaa
     9241 gtgactccag agtggttccg gatctggcgg aggccctata tgcggtatta tgccccgtct
     9301 acaaaagacc ctttggcata gttgccaaag ggattggagg gcaatcaaag cgggcccgac
     9361 ctcactctcc ccaaactttc gtgagcttta gcttccagtt ccgattacgc tatgctctga
     9421 tcgacggaaa tcgctggaaa ccaatcgagt acaaagaaca gggccgccgc aattgacagt
     9481 aattgaggct caagattgcg gtcatgctga tttgatctgc ccattatcgc caggtcgaat
     9541 ccgtcctcac agtcgcttgt ctttctcagc acgcacattc attcaaagtt cttgaaatct
     9601 ggccaatcgg taatcagtga agcccgtctg ttcttacaaa tccagatcgt ctggttgttc
     9661 tcatatggca tgcaatagcg atgcgtgaaa atgccggcag gaatgacctc gccgtagcat
     9721 tctttcatgg catcttcgga tccgcccaag cggatgacta cctcgccgtc agaattctga
     9781 catccccaca gccagtaatt attatgcccg ctgatagccc tgggcagatg gtactctccg
     9841 ccaaaatagt cgattgcccc ggcttctccg tagttgttgc agaggatgac gcatttggac
     9901 tgctcttccg gcgtgagccg ggcataagcc tccgcgacgg ccgccgtcat ctccttccaa
     9961 ccaaacatat ccgcataata ctgcgggagt tcactgagct ctttcttctc cggcgtggag
    10021 gggccgatac cgagtgcccg cgaatacgcg atgaaggtat cgaccggtaa cagggcaagc
    10081 gtcaacggta gtggcactag gccgcccaag attactgaag caagaataac aggcctgagc
    10141 catcgccact tcagccagag aacgaacttc tcgacagtaa acgatcccag agcaaagagc
    10201 ataggggaca tcggccccaa gtactccgac ttactattct tgctaactat aagaatcagg
    10261 aagaccgcta agaagatgat cggtagaacg cggaattgcc ttgttgatct tgacaccagg
    10321 aaatatgtca gtcccgcgaa ccagatgggg aatgagaccg gactcatgtt cagtacttgc
    10381 tggctgaaca tgtcccacgg tgacaccgac acgtacttat cggcgagcgc attgctcatg
    10441 aattcgagag tggggaagtt attcgctatc tgccatatta tatgcgggag gaaaagaatg
    10501 acagaaaggg caactgcaaa ccacactctt cgtgtcagca gaagggctcg atacggagtt
    10561 gcgagcagcg cgatcagcag tccagtgccg agccatagaa cgctgatctt gttcatcaat
    10621 cccaggccga gtacaattcc gagcagaatc caatgccgcg acagatcgtg tttggctatg
    10681 aggataagga gatagaacgc gagggtccaa aggagggtat cgaaggagtt cattgagaag
    10741 tgtgcattga tcgcaagtgt caaaggtgca aggagcgcac agattgacgc gaggatttga
    10801 gagtatttgc cgccgccgag ttcgcgtgtc atcagaccag ccaggaggac gacaactgcg
    10861 ccggtgacag ccggaagtag ccgcagggca aatagcgagt ctcctagaag cagacgattc
    10921 aaccataata tcgcgatgga taacggtggc tgatcgacat aaccccacgc catgtgatcg
    10981 ctgcaggcga tatagtaaaa ttcatcgcgg aacatcccat aaccaccagt gatattgacg
    11041 accagatgaa gacaaagctt cacgagggca atcgtcagca ggattgcact gccgtctcga
    11101 acgaagtggg atgatgaaaa gaaacgattg ggtgccagat tacccggaca catatattgc
    11161 gccctccggt ctgacgtatc ggatggagct cccgtgaatc cacaggctcg ccatcggagt
    11221 cctgcctgcc tgactcgccg caactctcac tgattatgac ccttctgacg agttgtgtac
    11281 cattactctg gaatatacga aaaaaacaag gtcggaggta ccccgcgtat aacacctcgg
    11341 aagtcaccgg cgtcttggag actaatgggt aggccgatta tgcagccttg gcctagtaag
    11401 ggaaaatggt tattcaatgg cggcccgcgg tatcactctc cccaaacttc tgtgagcctc
    11461 tggtttccgt tccagttacg ctatgcttga atcaacggaa ctcgctagaa aacagtcaaa
    11521 tacgcggaca ctgcttatcg tcattgcgtt gttggttgta agagagttac ttgtggctga
    11581 cctcacatgc atccgccgat ggagggaagg agttagtcat gaagcgacga ccactgtgta
    11641 gcacctcgtt aatgttatac gccacgcagc atgtcttctt acgaggataa tctatgagaa
    11701 ccacatttac tgctgcttta gctgcctgcc ttctatctgc cctactttac gcgcagactc
    11761 caccgccctc cgctcaagta gagggttcat gggtgaaggt cgcctgccca ttcgattcga
    11821 gtaaagcgct gcttgctgtc acctgcggcc gactgaaagt gcctgaaaac tacgatgaac
    11881 ctaaaggtcg atcgatagag attgcattca tggtagttaa ggctcgaaag aacatagatc
    11941 cagaaaaccc agtccttttc ctaaacggag gccccgggca gacaagcctg ttcttcgcag
    12001 agactctcgt aacaactccc ctcatctatg atgttgtggt tgatagagat tgggtgtttt
    12061 tcgaccaacg aggaactggc aggtcgattc cgcagctata ttgccccgac gatcaagaca
    12121 gtccttttga tttgaaaaag tgcagagatg gtctaataaa gcaagggata gacctttccc
    12181 agtacaacag cgcacgaagt gccagtgaca tggaagcgct tagaaaagca cttggcgtga
    12241 atcagtggaa tctctggggg atctcctacg gcagcagact ggcaatgaca atggcgcgct
    12301 actacccctc gagcgtccgc tcgatcgttc atgacgcccc tggcttgcct gggggtcagg
    12361 aactcgtcga cgatgctctc ggaacggacg tcgcgctgaa taagatattc tcgaagtgtg
    12421 cgatggattc agcgtgctcg tccaagtttc cagaactgcg gagccgattc acagcggcgc
    12481 ttctccgcct caggcagcag ccattattgg tcggcgagaa gcggtttgac gaccatgcgg
    12541 tggttcagtt ctttcgccac tggttctacc ccaggggtta ttcgacgtat gaacatcgcc
    12601 tacagaacct cctgattttc atggatgcgg ctgctcgcga cgatggccca ctcatggacg
    12661 aaactataca aaggatgaga aaggaggaag ggcttgataa ggttcggccg cccgagccga
    12721 tttacgcccg acagagtatc gcacagaacc tctctgttta ctgcaacgag aagaaaccgt
    12781 tcgagtccat ggatgaatat cagaaagcgg tggcaagctc cgagatcgtt cgttctcttc
    12841 tcgcggattt tggagctcct tcagactgca ctttgtggcc ctccggcgaa gctgattcaa
    12901 ttgaaaacac tcgcgtatat tatgacgggc cgcagcttgc ctttacgggc gagctggatg
    12961 catcctcgtc gggccttgcc ggctataaaa tcgagatgct ctatgccaat gcactaaacg
    13021 tcgtcttcaa gaatggctac cacggccagt tcccgacaga gctacccaat tcggaggacc
    13081 gtgactattg gctgtgtgcc ttgcggctgg cgcaccagtt ttttgctgat ccgcaacgaa
    13141 agctggatac cggctgcgcc gaaacacgac ggttgcgctt ggtaaaatga tcaatcgagg
    13201 tggcggctac aacagcaata gcaggtaggg ttcaggcgtt cactagcgaa tgacttcttc
    13261 aaaatcgatc gtcgaattga gcactcctgc agcgtgaatt atggtatcaa cgttgatcac
    13321 attctctctt gaaagttgcg cgagtgcaaa agctataata tgagcaccaa aactgcggcc
    13381 gggttaagca atcgggataa aaagcgggcc cagtatcact ccccccaaac ttctgtagta
    13441 ttctctttgc gggtgcacat caggtactcg acggcaagcg gaacacgctg gaaaacaata
    13501 gaatagcggg gcactactaa ctgccaaacg catgcaatgc ggagcctcga agtgtgctct
    13561 cctcgtcttc ctgaaacatt tgagtaactg cttacgtaac gcttaccacg gagtccacga
    13621 tgagggattg agccgcctaa ccgaggagtt catgtgaaga cgcgaatgct caagaccagc
    13681 tgcctgtcgt tggcactgct aatgctccct ttgctgatgc atgcgcagga gaccggaacg
    13741 gttgccagcc ccgagacggt gtttgaccac gtgtggcaaa cagtggatcg tacttacggc
    13801 cagtttggta gtaaacacgt cgactgggcg gccctctata gggtctatcg tccacagatc
    13861 actgccgaaa caagcgacgg cgacctgcac cggatcttgc tggagatgct tggacatttg
    13921 aacgatgccc acgtgtgcat tcagaccgac acgagtcgca cctgctgcgg tttgaccgag
    13981 ggtctgccgc gtgtcggctt ctcccgggaa ttgatcgagt cccactacct gaagggaaca
    14041 gcggacacgg cgctcgacgg ccgtctgttg agcggctggc tgactgatca gatcggctac
    14101 atgtatcttc gcaacttcaa gagcaagccc gacgagttga gcgccgcgat agacggctgc
    14161 atgaaaggat ttgctgctgc ctccgcgctg attgtcgatg ttcgcgataa cacgggaggc
    14221 aagtcagaga acagcgagct ggtggctgac cgattcgccg atcgtaaacg gcatttcgcg
    14281 acgagtttcg atcgctacgg cgaaaaacac gacgattatc taccgcctac ataccggaat
    14341 atcgagccgg ccggcccgat tcagttcact ggccccatgg tagtgttgac cgaccgccac
    14401 acggcgagcg ctgctgagga gtttgtcctg gctatgcggg tcctgccgca tgtcactatt
    14461 gtcggcgacc ttactgaggg cgccttagcg gctcagtatc cggaaaggct gccgaatggc
    14521 tggacgctat gggtcgctca caagtacacc attgatcttg agggggtctg ctgggatggg
    14581 gtggggattc ctcctgacgt gcggatcaca aacacccagg ccgacattga ttcaggtcag
    14641 gatcgcgttc tgcagtttgc tctcctcttc cttgaaacgg gcaggccgga accacaaatg
    14701 gaaccagcca gtctcgatgg aatccggacc tccctcgtgc aggaattcaa gatgaacctt
    14761 gataagtccg gattcgaatt cgccgtaaac gacctcagaa gcaagattaa gggcggggac
    14821 cagcgctact ttttcagcgt cgtggaaatg ctggagttgt cgcgagaata cgttagcaga
    14881 gatgaattca agcaggctat cgaactactg gaaatcttgc gggcagaata tccgcggttt
    14941 gcccacacgt atgggttgct ggcagccgcc tatttgaact ccgaaaacca ggaggccgca
    15001 aggacggttg tcgccgaagg agagcgaatc aaggccatgt attcgtggga gaaaccgctc
    15061 ttggaaaaag taaagcaggc acttgaagaa cagtaggcag attgccaccc atgcccttgt
    15121 agtgcgaggc cttcaccctg atgcgtgtac cgacaggctg aacccgatac ccaggatact
    15181 ggtatgccgt tctgtccaca aaagccgtgg ttgtaaaggt tccattgtgc gtcttccatt
    15241 tgtccgttcg tatctgttag ccgccatatt gcttgtccca ctagccccga ccacgtcctg
    15301 cggtgagaat gacgactacg atcggtttgc ctgcaaagtt gtcaccctgc tcgcccctga
    15361 gatagtacct gacgagccgg gtgcggcagt ggtggtcact cagcagggca aggtcatcta
    15421 tcgtcgttgc ttcggcctgg caaaccttga acacagggtt cctattacac aagaaactgt
    15481 cttcgatctg gcctcctgcg ccaagcagtc aagcgtgcta aagtcctatg gcatgggcaa
    15541 ctcgaatgac cagtctactg tccggattga atggattgac tatattattc tgatctctgc
    15601 cgccggactg cactgctcgt aggcgtttcg caagtcagtg tcgtctacgt gaacgtatcg
    15661 ttccgcacac gcagggtcga gtggccgagc gcacgctgca ggactcgcag tggcccacct
    15721 gcgcggacga atgctgtggc cccggggtgg cgtatcggat gcgagtgcag gtgtagcccg
    15781 atcttacggg caggattagt gccgtcaggc aacttgagat aacacgagtt tacggagagt
    15841 aaggttggct atttttgtaa gcgtataacc gattgcgagt taaggggata gataaggcgg
    15901 gccctcgtag acgcgttccg caactgggtg ataggcagtg cggcgtagcg cgtgttcggc
    15961 agctggcgat tctcagggaa tgcgccaatc aagctctggc tgcctgactg aggatgtgac
    16021 tcacgggtct aagcgcttct atgatcttct gtttgttccg tggattggta ttaacaacca
    16081 gcggactcgg atgatagcat tcgaatatcc tgatgtagtc atattgactt tcgagtttcc
    16141 ttttcactct ctgggctttc ttaccaacga gcacaataat ctgtaggttc ggcaagagat
    16201 ctataaggct attcaaataa ggcagccccg cacggatgtc atctgctgtg gccggtcgta
    16261 tcttcttgcc ggtaccgata taccagggca caacattcca agtaatcgtt ttctctcttg
    16321 ggataccggc ctccacgttg atttcaaaga agttcttcgc cgactcatcg tcgttattcc
    16381 gagaaatgaa accggacctt accgcatttt gccccggtgc ctccaatacg aacaggcatt
    16441 gcgctctggt gccaccgtcc agaggatcga agtacggaat gctcttgtct ggatgttggg
    16501 aaatgatagc atccacatat cgtgtgagag gggcgatatg ctcgtgcttg agcagtctct
    16561 ttcttgctga tcgctccgct tcgtgtttaa ggctcttggg gcaatctttc attgatccca
    16621 cagtataagg taacaaaatc cgagatcaaa tccactctgg agcaagcagg cgcaacacta
    16681 ctggagcggc taaggtggtg gaggtcaatt agatatgaga atggcggggt tgacgagact
    16741 cgaactcgcg gcctcctgcg tgacaggcag gcgttctaac caactgaact acaaccccgg
    16801 ctcatcgaac aatcgctcgg tcaatccacc ttgcgtctcg cctgaaccga tcggcctcgc
    16861 ttgaaccgat cggcctcgcc cgccgattcc catctgtcca gcatcgcccg ggggccaccg
    16921 gcatctccca tactcgggga gtcgaccacc ccgcgccggc gccgatcagt cgcgcatgcg
    16981 cgggtgagtc aatcctgcac cgtcagacga agtctctctg ggcggtacaa gattcgaact
    17041 tgtgaccccc tgcgtgtaag gcaggtgctc taaccagctg agctaaccgc ccggtctcag
    17101 gttcccctta cgggcgcctg cggcaacatc gcaaggacca tcgcggttcg ctctgttccc
    17161 cgttgtctcc aggggcaggc gaatcggcaa cggcccaata gtataatgaa ggccggcgaa
    17221 aatgcaagtg caaagagggg ctatcggcaa cggatacgtc aaaaaaaccg ggcgcgcccg
    17281 gcggcggtcc ggccctcggc gaaccctttg gcccgccccg cgttatcgga ttaaaagttg
    17341 acgccggtcg gcgcgcgcgg ctatcttgag caaaagtgag gacccgctat ggcagttgac
    17401 ccgtcgatct tcaaagccta cgacatccgg ggcaccgtcc ccgaccagct caacggagac
    17461 ctggcctacc gcatcggtta tgcgctggcc agcgtgctga aaccgagatc catcgcggtc
    17521 ggtcgcgata tgcgcgtatc ctccgacctg ctctttgagc acctcgcccg ggggataaac
    17581 gactatggcg ccgacgtgat cgacctcggt ctcatttcca ccgacgccct ctattttgcc
    17641 gtcgggaaat tcggttacga cggcggggtg atggtgaccg ccagccacaa cccgaaagaa
    17701 tacaacgggt tcaaggtctg ccgcaaagag gctgtgccgc tctcgggcca ggagggcctc
    17761 aaccaggtgc tcaggtttat ccaggacggc gaggagatgc cccgctcccc ctctccgggg
    17821 gtggtggcgc gcaaggatat cctctccgaa tacgccgacc attgcctttc gttcatcgac
    17881 atttcccgcg tccgggagtt ccacgtcgtg atcgatgcgg gcaacgggat cgccgggcgg
    17941 accgtgccgc cggtcctcga ccgccttccg atccgcacga cgcggctcta tttcgagctg
    18001 gacgggactt tcccaaacca ccccgcctcg ccgattgagt tggagaacct ggccgacctg
    18061 atccgggcga ttgcggagac gggggcggat ttcggggccg cgttcgacgg cgatgcggac
    18121 cgcatgttcc tcgtggaccg caagggccgt cagatcggcg gggacatggt gacggcgatg
    18181 gtggccaagt cgctgctgaa gagacatccg ggggagacga tcctctacaa cctgatctgt
    18241 tcgcgggcgg ttcccgaact ggtggagcgg atggggggga cggcgatccg gacgcgggtc
    18301 ggccacgcgc tcatcaagcc gctcatgaag aagcacaacg cgattttcgg gggcgaacac
    18361 tccgggcatt tctatttccg cgacaactgg ttcgccgact ccggcctcat cgcgttcctt
    18421 gtctgcctcg agctgctctc cgaggagcgg cgaccgctgg acgaggtggt gcgcgacctc
    18481 gatccgtatg tccgctcggg cgagatcaac agccgggtgg ccgaccagaa ggcgaaaatc
    18541 gaggcgatcg ccgaggccta cggcgacggc cggcaggacc ggatcgacgg cctcacggtg
    18601 gcctacgaca ccttctggtt caatctccgg ccgtccaaca ccgagcccct gctgcggctg
    18661 aatatcgagg ccgacagccc caggatcctc gaggtgcaga aagcgaaact gctgggcctg
    18721 attcgctcct gagtatggtg gacacgccga tccggttact agtggtcgat gacgacccgc
    18781 tcctgcgcga gctgctgcag gacacgctcg acacgctcgg gtaccgggtg acggcggcgg
    18841 ccgacgggat cgaggcgctc gagcggctcg cccaggggtc gttcgatctc gtcatcaccg
    18901 acatcaagat gccgggcatg gacgggatcg cgcttttgaa gcgggtcaat cgcctctacc
    18961 ccgatctccc ggtgctgttc atcaccgggg tcgcctctcc ggaggtgatc gggcaggcgt
    19021 cgccggtcgg ttttctggcc aagccgttcc gaatctcgca catcgagagg ctcatttcgg
    19081 acgcgctgca ctggcggaag gagcgggtgg agcatccgat ccgccgggtg atggtggtgg
    19141 acgacgacga tctgtttcgg gagatgctcg ccgacacgct caccggccac gggttcacgg
    19201 cgtgcgcggt gcagaacggt ccgcaggcgc tgtccgagct ggagaacggt ccggttgacg
    19261 cggtgatcac cgacatcaag atgcccggca tggatgggat cgcgctcatg caggcgatca
    19321 aggagcggca cccggagctc ccggtggtgc tgatcacggc gtttatgagc gaggcggagc
    19381 tcgggccggc gacgcccgcc gcccgggccg acgggtttct ccgcaagccg ttcgagatcg
    19441 atcgcatcct cgaacttctg gattccctcg caccctcgcc ggtcgacggc cgggcgggtt
    19501 catgatcgga ccgcgctgac aggcaggggg tggacatcaa accacatttc cgcacctccg
    19561 gggaggagag tccgcgcatc ccggccccgc ggccgggcga caggcggagg tgcggcccat
    19621 gacgccgcgc acgcgacgga tgctgggcgg ggcggcgctg gtgttcgcgg ggctcgcggt
    19681 cggattcgcg attgcggtct gggcgacgct ggacgggacg ctgccgccgt gcgacggcga
    19741 aatccggctg gctggactcg acgggccgat cgagatcaca ttcgacagca tgggcgtggc
    19801 ccaggtctgg gcggagacgg cccacgacgg tctgttcggg ttgggctggc tgcacgcggc
    19861 ggaccggctc ttccaggccg atttcacgcg ccgcgtggcg caggggtggc tgtcggaact
    19921 gctgggcgcg gccacggcgg cgttcgacag cgagcggcgg cgcgacgggc acgcgcgcct
    19981 cgcccgggcg gccctcgcaa aactctcgcc ggagaaccag gctcgcctcc aggcgtatgc
    20041 cgacggaatc aacgcctatc gtgcgcgcgg ccgccggccg ctcgagtacc tcctcctccg
    20101 gaccgaggcc gaggaatggc gggtgtacga ctgcctgacg gtgctgagtt tcatgacctg
    20161 gtactcggac gcccttcaga accgcgacca ctttttcgcc gaactagtgc gggaaggtcg
    20221 cgcgggcgtg ctggacagcc tgccactggg ttacccctcg ggggcgctgc cgccggggtg
    20281 cggggagcgg gccgaggcgg cggggcggtt gaacctcgga gggggggcgg aggcgttcac
    20341 cccggcgcgg tcgtccaacg cctgggccgt ggcgccggcg cggagcgcct cgggcaaggc
    20401 gatcttctgc agcgacccgc acctggaact gacgcgcctg ccgcagttct ggtacgcggt
    20461 tgggctgcac acccggcggc ctcagctcga tgcggtggga gtcacgatcc cggggctgcc
    20521 gttcgtcgtc atgggtcaca acggtcaggc ggcgtgggcc ttcaccgccg gaggagtcga
    20581 cctcgtcgat tactacgtcg agcgggtgaa ccccggcgac ccggaggagt accttgagcg
    20641 catcgatccc gcgcggggcg ccgactcgct cgtctggagc cggatcgagg tcgtccggga
    20701 cacggtcgcc atcgccgggt ccgactcggg agcggtgatc cagctgcggt tcacgcggca
    20761 cggggcggtc gacccggagt ggtcgggccg cgactcgctc gtggtctgtc ggtgggcggg
    20821 gcttgaggcc gacctcgatc gggcggcgac agccgggtgg gagctgatgc agacccgcac
    20881 gttcgcggag ttccgcgggg cggtgacggc gatgggcgcg ctcaatgcca attggctcta
    20941 tgcggatgcg ggtggaacga ttgggtacca gctcggcagc ccggtgccgc gccgcggcga
    21001 ggcgtcgccc tacgcgccgc tcgcggggtg ggcggccgaa gacgactgga aggggtacct
    21061 ctcgggcgac gaacggccgt ggtgcgaaaa cccgcccgag ggatggctgg ccaactgcaa
    21121 caaccggacc gcggagcgac cggacatccc cggatcgtac gcgatcgatc gcattgtgcg
    21181 gatcgaggcg ctcctgcggt cgcaggagcg gttcacggcc gacgacatgc ggcgattcca
    21241 gcttgaccgc atcgacgcct acgcgctgcg gtggaagggg ctgatcgact cagtcggcgc
    21301 cgcgatcggc gacacgaccg gcgcgctcgc ggccgtcgcg tcgtgggacg gttcggcgga
    21361 cgcagcctcg cgcgcggcgg gtctggtccg ggtgtttctc atgcgcctgg ggccggccgt
    21421 gttcgccgac gagctgggag aggggagtga gggtctcggt actgccgccg tcgaccaggt
    21481 atttttcggc ggcgacagca cgtggctcga tgaccgcacg acggccgggc ggatcgaaaa
    21541 ccgctacgag atcgccggcc gcgcgctccg ggaggcgctg gacatcgccc gaggtcggac
    21601 gtggggggaa atgcagacgc tgcagatgcg gcatccgatg gcggacgccc cggtgatcgg
    21661 agggctgatc gatctcgcgt tcggtccctg gccctgggga gggacctcgg gaacgctgaa
    21721 ctcgtccttc tgccggcagc cggagccggg gcggttcgag acgatcgtgg ggccgagctg
    21781 gcggtttatc ctggatttcg ccgacgtcga gggcgcgctg atggtgcttc cggccgggaa
    21841 ctcgggccat ccgctcagcc cgcacttttt cgatttcaac gagtcgtggc gaaacgggcg
    21901 ctactggaac gtgccgatcg ggcgggaccg ggtgtaccgc cgggcagcct cgctcctcag
    21961 gctggcgccg ggggacacgg ttcagccggg gtcggagagg agcagcgcct tgatccgatc
    22021 gaactcctga ttggcgacgg cgtaatcctc gaggcggccg acatcgagcc agtagcccgc
    22081 gatgggatac gtggcgaccg gctcgccgcg cgccagcagg gtcgccatga gatcgtcgaa
    22141 tccgaatcgc tcgccgcgcg ggacatagcg gaggatctcg cgcgagaaga cgtagacgcc
    22201 ggccgagacg gtcacggtgc gcgccggttt ctcgtggaag gcggcgagcc gcccctgcgc
    22261 atcggtctcg atgacgccgt agtcgacccg gttctcccgg cggcaggtcg cgaccgtcgc
    22321 cacgacgccg cgctcgcggt ggccttcgta caggcggcgg aagtcaaggt cggtgaggat
    22381 atcgccgttg accaccagga aatgttcggg caaatcctcc atcagcgtga gcgggccgat
    22441 ggtcgacagg ggacggggct cccgggagta ggtgatctcg acgccgagcc cgcggccgtc
    22501 gccgagcgcc gcttcaatca ggtgggcgag gtggctcacg gccaggcgga ggcggcggat
    22561 gccgcgccgg cggagggtgt gaatgaggat ctcgatgatg gggcggtcgc cgacggtcac
    22621 gagcggcttg ggaatctccg ccgtgtgggg atagagccgg gtgccctggc cgccggcgag
    22681 gatgatagct tcgatatcga cagactcaga catcggcggc ctcacacgtt gcggtagcgc
    22741 gggttttccg gatcggtgat gagaccggcc gtcagcgcgg cgacgatctc gcggatgccg
    22801 tcggccacgc gccgccgcgg aatgaatccg agggtgtcgc ggatgcggtc gcagttgacg
    22861 cggtagtcgc gggggtcttc ctcgcggcgg acgaaggtga ccagcccggc gcggtcgggc
    22921 agatgcgcga ggatcatcgc cacgagatcc gcctttcgga aattctcgtc ggtgtggccg
    22981 acgttgaagg cccggcggtc gatcgcctcc gggggcgccg cgagagcgag acagcaggcg
    23041 gccgcgaggt cggcgacgtg gcagtacggg cgccaaaact gctccccgta aatttcgagc
    23101 ggcttgcgga ggaagagatc gcgggtgaac tcgttgacgg tcagatcgaa gcgggggcgg
    23161 ccggagaggc cgtaggcggt ggcgaagcgc aggcagacgg tggtcaggcc gggggcctca
    23221 agggccagca ggcggcgctc gaaagcgact ttgagttcgg cgtagagcga gatcggcctc
    23281 agcggcgcat cctcgtcgca caccgcgccg tccggcgccc gcccgtagtt cgagcaggtc
    23341 gaggcgaaga cgaagcggcc gacgccggct gcacgggcgc gggcgaggag gagttcggag
    23401 gcttcgccgt tcacggccgc ggccagttcc ggctgccggc ggcaggcggg atcgccgacg
    23461 acggcggcga ggtggacgag gctgtccacg ccgcgcagtg cggcgtcgac ggccgactgt
    23521 ttgcggatgt cgccgaagac gaactcgaag gagggatcgc cgagcactcc caggagagct
    23581 tcgccgcccc agctcaggtt gtcgatggtg cggacgcgca ggccggcccg caggagcacg
    23641 cggacgagga cggatccgat gtagccggcc ccgccggtga cgaggactgt ctgcggacgg
    23701 tgcgtcatgc ggcgccgcct atgatttcag cgtgctgagc acgcggcgcg cctcggcgag
    23761 atcatcgccc gccagtcccc ggtcgatggc ttcctgcagt cgcagccggg cctcgtcggg
    23821 cttgttctca aggtagagcg ccatacccag ccggaacagc gggccgggct cgccggcaaa
    23881 cgaccgcgtc atcttgatcg cctcgccgcg ggcgaggtcg taccggccgg tcacctggta
    23941 cacgtagctg aggttgagcc actcgcggat accctggttg gagtcgaaga gggcgcggcg
    24001 ggcgaaatcc tgcgcctgcg ccggttcgcg cccctgctcg gccaggatgc gcgagagcca
    24061 gtaggtcgag gggacatgga attggtcgcg gtcgagattg gcgcggagag tggcgattgc
    24121 accgtccacg tcgcccgtgc cgcagagggc gcgggcgcgg taggccttgg cgtccagcag
    24181 gtccgggaga actttcagcg cggcatcggc cgccgcgatt gcccccggat agtcgccgag
    24241 ctccagtcgc agttgggcgg ccagggccag aatgtggggg tgctcggcgc cggtttccat
    24301 gatccattcg gccgcgcggc gcatcggctc gcggtaggcg cggaaagtca ggacctcgaa
    24361 gaagttctgg aacatcgctt cgtcgccgag attctgcggg gcattcatca ggtacacctc
    24421 gagcccgcgc tcgggctgcc cggcttccgc caggcatatc ccctccagca tggcgagggc
    24481 ggggaagcgc tcggggaagt gggggtagcg gtcgaacagc ttcgcggctt cgtcgaatcg
    24541 gtcaagccgg cggagcatgc cgacccatcc ggtgaatgcc ggccggtgcc agggattgag
    24601 ttcgagcgcc cgttcgtaca gggcggccgc ggtgtcgtac tggtgcagga tgatgtcggc
    24661 gtagacgtcg ccgagttccg cgagccactc cggctggctg ctgtggagcc ggtgaatctg
    24721 ctcgacctcg gttgcgaaga tgtcgaattg ccccgattcg aacagcgccc aggcgtgccg
    24781 ggcggcgatg tgggggcggc tccagtacat tggccggatc tgctcccact gcacgagcgc
    24841 ctctttgccg cggagccagt cagccgcctg cagcgcgagg aggtagaaga ccgcttcctg
    24901 aaattcccgg tcatacccct cgttgatcag gaaggtcgag acggtctgga tgttgtggag
    24961 gaggacgatc tcgtttgagt atttcgtctt gatcgccgac tcgtacatgc gcatgctcag
    25021 ttgtccgccg ccgaggccga tgacccggtc gatcgcctcg ccggccggca tggctctgcg
    25081 ggcggcatca tagcggaacg cctcgagcat cgagaccacg gtctcgggga tcttcgcccc
    25141 gcggagccgg gcgagcaccc ggtcggcctc ggcgaagtag ttgtgccgaa cggccagttc
    25201 gaagtgctgc tggagagtcg ggacgtctcc tctggagagg tcgacagctt tgcggctgag
    25261 ggtgacggcc gagtcgatca ggccggcggt ttccaggtac gaggcggcct ggaggtagac
    25321 ctcgggggag gatccggctc cgaccagggc ggcctcataa gccgagtcgg cggcgttggg
    25381 ctggtcggcg gcgatgaacg cgcgggcccg aatcatagct acccgggccg ggtcggctcc
    25441 ctcggcggcg gccgaatcca aaaacgggtg cgcccgccgc ggaaggttgc tatcgatgag
    25501 ggccagggcc aggatcaccg ccgcctcggc gtcatgcggc agcaattcgc ggagggcctc
    25561 cgcctcccgc agagcttccg gcagcagtcc gagccgggtg tagagacgcc aggcgccggc
    25621 gtgagcgtcc gtgaaggagg gaaaagtgtt ggtcagggcg acatagacgc cgagcgcatc
    25681 gtagtgctgg agctggcgct ccaaaatata gccggaggcg aagcgggccg agggcgcggt
    25741 gggctcgaga tccgtaaggc cctgcagcga tttctgcgcc gccgggaaat cgcaggcgtt
    25801 ggcggccgca atcacctcgt cgaacaaccg ctgctttttc tcctgcggcg actcggagca
    25861 gccgagagcc gccaggagaa gcgcggacag gagggttgcg gtgaggaatc ggggcatagt
    25921 ctgtctttct ggtaacgtgg cagttttcat ccgaccgggt ccgaacaaac cggaatttac
    25981 acctttttac ccggccgcca acaattattt cccggcgggg ccggcgggtg agggaatcgg
    26041 cgcgcggcgc ggggggcgcc gcggcggagg gcggggcgga tttttttggt tgattttcca
    26101 atcgccgcgg caaaatagta cggcatggcg cgtatacaga aacccccgat ggggcggctc
    26161 ttcgtctcga tcatctactc ctccatggat gccctggcgg acgggctgcg ggctttggaa
    26221 cgcaagttcg ggcgcgtgca atacgaaacc gtcgagatcg cctgctccga ccgcgaactc
    26281 taccgcgagg aaatgggcga gaatctccag cgccgtctct tctcgtttga acggctcttc
    26341 ccccgcgacg cgcttccgga gatcaagggg gtgtgccacc gggtggagcc gcagttcgcc
    26401 gacgtcgtcg gcgacttcgt attccgcacc gtcaatatcg atccgggcat tatcaccccc
    26461 gaccaggtgg cgaccgcctc gcaccgcgag ttcagccacc ggctctacct gcgggaaggc
    26521 gtattcgcgc aggtcgagct gatctggtcg catggccaat tccaccgcct cccctggacc
    26581 aaaccggatt actgcgaggg ggaggcgatc gagttcttcc agcgcgtcag agagagcctc
    26641 gaggcggcgc cggagtatga gcagccggtg agcgggtgag aatatatatg agcatctccg
    26701 caaaaagctc ttgacagcgc ggcgcccttt tcttacactg ccattgacat cgcagggttg
    26761 ttcaacaagg ttctgtgttt gctgaaggtt gatcggagag gttcggagcg acgtcagggt
    26821 tgtgtgtctc cgacgcttga ttattctcat gcgggaagag tgctgctgac tacggctgca
    26881 gcccggtgac cgaaggccgg ggtgtattcg acgggaggag tcttgtcctc agcgcccgtc
    26941 gccgcggccg gcggccgatc attgggccga acggcccgaa gcggacgagc ggcgcaggga
    27001 ttcgcgcacg cacacacaac tgccaaaggg acttgcccac aaagtgaaac gttgaaagac
    27061 gttggaggag gtgatgccgg caacgatcct gacttcgcta tcggacaaaa caaccttcag
    27121 caatgtcgat tccggggagg gttcgtccct ccccggaatc cggacaaaga gccgaaacac
    27181 agaatgacca gaggacaaaa atgagaaact tggttaaggg aatcgttctt gccgtcgccg
    27241 ccctgctgct gctgtggggc agcgcctcgg cgatggtgga gggcgtgcgc gtgtcgacgc
    27301 cggacacctg ctggtaccag cgggggttca tcgccttccc gatcgcgatc gaatggacga
    27361 cctggacgca gcacccgccg accgcgttcc cggacccggg cccggtcgat tttccctacc
    27421 gctgcaacga cgccgagtac tgggtgcatc cgtcgcacga cgtgctcgtg cggccgaccg
    27481 tgaccatcgg ctatcccggt ctgacgtact gcaacctgcc gacggatctt cccgattccg
    27541 ccaatccgtt cggcgctccg gacgagacga tgtgcggttt cgagatgacg gtgacgtttg
    27601 acgaggacgt cctccaggcc gtcaatgtgt ccaacagcga gttgctcacc gacgactggg
    27661 gctggggacc ggtgttctac gagatcgaca acgcgcgcgg catcatccac atcgcctcgg
    27721 cctcggccga ctgcgaggac atcaccggcc tcgacgatcc gacgacgctc atctggatcg
    27781 gtttctacat caacgggagc cccggcgaaa atgccgatct catggtcgag gaattcacgt
    27841 acaacgagac cgatccttac tacgtgttca tcgataatga gcagtattac caggacgtgg
    27901 acgagggcat ccccgggtct gagcaggtgt acctcgggaa ccagtcgatc ggcgacttcc
    27961 tcgtctgcga gttcctgagc gcctcgggcc aggtgctcta catggccaac ggcgtgccga
    28021 tctgcggtgc ggacgtcacg ctctactacc atccggacac cacgctcgtg atcgcgccgg
    28081 tcatcgacaa cctgacctac gagacccagt gcgtgatccc gtgcgaggat gactgccggg
    28141 ggtcgtacta cattccgggc atcaccggcg gctacgacta ctcgctcggg gcgtggcacg
    28201 acgacgagga tgcgtacccg atcgccatca cggccttcga cgcctcgctc atcctccggc
    28261 acatcgtcgg cgccttcgag ttccgcgagc cgttccacta cattgccgcc gacgtgtcgg
    28321 gcagctgcga agtgagcagc atggatgcct cgctgatcct ccagtacctc gtgggcgagt
    28381 ttgactactt ccccaagaag gcggacgagg agactaactg gctgttcgtc accgactact
    28441 tcaccaagtc cgatccgtac ctctgtcccg aggagcagta ctggttcgag ccgatggagg
    28501 acaaccaccc ggggcacaac ttctgggcgg tggttctggg ggatgtgtcg ggcaactggg
    28561 gtgtcgggat gacgatcccg ccggcgaagg agagcggttc gccggtggcg gtccgactga
    28621 tcggcgaggc cgacggcttg gcgacgtacg agctgtcggt gccgggtgag ccggcctttg
    28681 cgttcgattt cgcggtcgag gcctcgaccc ggcttgtctc tgtctctccg ggcgaggact
    28741 ggatcaccca gatcgccgag caggcggaga gccgccacgt cgtcgcggcc ggcgtctccg
    28801 cgaccacagt gctcggcacg gtggaagtgg cggccgacag ggagacgctg cagttgcggc
    28861 gcctggtggt gaacgaaacg aactacggca cggtgacggt ggcgctcaag ggcgagccga
    28921 ccgcgccccg ttcgttcgcg ttgcacgagg cctacccgaa cccgttcaac ccggccacga
    28981 cgatcgggtt cgcgctgccg caggcgtgcc aggtgacgct gacggtgtac gacatcctgg
    29041 gccgcgaggt gaaaatcctc gccagcggcg ccatggatgc cggcgaacac aatgtcacgt
    29101 gggacggcac ggatcgctcg ggccggacgg tcgcgacggg tgtctacctg taccggctga
    29161 cggcggacgg tttcacgcag tgccgcaaga tgatgcttct gaagtaacgg tgctccatgc
    29221 gaacgccggg agcggccgag ggccgctccc ggcggaggaa agccatgcat aaacgactca
    29281 ccttcttacc ggtgctgctg cttgcggcgg gtgtgtcggg tgttcagggc gaggtgaacg
    29341 gcaccttggt gtgcgacgag ctggccgata cgctcctggg cgtatcagca attctctggt
    29401 ctatcagcga ctttgccgaa ccggtgtaca gcctggaaat tgaggcctgt ttcgatcccg
    29461 cctgcgtgcg gatccgcgac gtggactata ccgcgtcact ggtggcgggg tggggggagc
    29521 agtttgtgga tcgcctggat gccgatgccg gcaccttcgc cctggcggtc gccggcaccc
    29581 tccccctggc ggccgattcg ggtacggtgt ttcgcctgac gctggctaag gcggccgggt
    29641 tgccgccgcc cgtgcccgtc ggccgggtca ctcgttttcg catcaacgag acctcgctgg
    29701 tctcgacgcc gcccggcggg ggagagacgc cccgcgggta cgacctcgcg cagaactacc
    29761 cgaacccgtt caacccgggc acgaccatac gcttcgcgct cgggcgggcg gaggcgccgg
    29821 tggtttccat atacaatcaa ctcggccagg tagtgcgccg cgatcacctc gggccgctcc
    29881 cgcccggacc gcatgtctat gtctgggacg ggacggacga gcgcggccgg ccggtggcgg
    29941 gcggcgtcta cctgtatcgg cttgagacgg ccaacttcac acggtcgaag aagatgatcc
    30001 tcgtgaagtg aggatgcgtt caccttgatc agaaagaact gccatgcgac gaactcagaa
    30061 tacactcgca atcatcctgg ccgcggcaat cgggctcgcc ggcgcccccg cgtggggcat
    30121 gagcctgccg ttccagtggg cgaccaaccc cgagccggtc accagcctgc ccccgggcga
    30181 cacgctgcgg atcggcctct acgtagactc gctcatgaag gccccgatcg gcggctggct
    30241 gcagggggtg caggtcaacc tggcctacga tgaagaccgg ctgcactggg agcggatgga
    30301 gtggaccggg accctgctgc agaactggat ccgcttcgtc ggctgcgact cggccttcgg
    30361 cgagatcgcc gtggccgcgg cgacggccaa agacagcatc gtggaaaacg gcttcctgct
    30421 cttcctggtt ttcaccaact gcagcagcag caatgtcatc ctgcagcccg gtgcgctgcc
    30481 gggtctgctg gaggtcgaga ccgtgatcct caacgaggaa cacgtcgacc cgtttccggt
    30541 ggtgggcgta tccgaaccgg aaccgccgct gccggccggg tacgcgctcg cgcagaacta
    30601 tccgaatcca ttcaatcccg cgacgacgat cgcgttccgt ctggccgagg ccggggacgt
    30661 tgcggtggcc gtctacaacg ccgcggggca actggtccgg ctcctgcgct gcggccgctt
    30721 gggcccaggg aaccacgccg tgatgtggga cgggcgggcg gaggacggtg cgccggcggc
    30781 ctcgggatgc tacctctatc gtctcgaggg ggatgtcgtg tcgccggccc ggaagatgct
    30841 tttggtaaag tagcggctgc gagtcggccg tcgttgttag accaacggaa gcaccccgcc
    30901 cgctgcggcg ggcggggacg ctaaaatccg gggccccctg agggggcggg cgggcttttt
    30961 tgtttctttt gcgggcccca ttaatcagat tggccctcaa aacagtcaag aaggcgcgga
    31021 ggccgcgacg cggcgcccgc gatcgcgccg ctgccggggg gcggagggga gcgcgccctg
    31081 gcggagggcc gcggccggcg gacgccggag tggcggtcgg cgccaaggat gtgagggtcg
    31141 atggacaagc tggttatcaa cggatcgcgg ccgcttaccg ggcagatcag ggtcgagggt
    31201 tcgaaaaacg ccgcgctgcc gatcattttc ggcgcgctcc tgatcgacaa gggggaaacc
    31261 gtcattcgca acgtcccccc gctccgcgat atcttcacca cgatcaaggt gatggagcac
    31321 ctcggggcga agatcacgta cgatccgaag gcgcaggtga tgacggtcaa tgccgagcag
    31381 atcacggaga ataccgcgcc gtacgagctg atgcgccaga tgcgggcgtc gttcctcgtg
    31441 ctcgggccga ttctcgcccg cctcggccgg gcccgcgtgt cgctgccggg cggctgcgtg
    31501 ctgggcgccc gaccggtgga tttccacatc aaagggttcc gggcactcgg ggccaaagtc
    31561 accgaggagg ccgggtacgt catcgccgag gccaagccgc tcgcgggggg gtcggtgtat
    31621 ttcgaccggc cctcccacac cggcaccgag aacctcctct tcggcaccgt cttcgcaaaa
    31681 cggcggacct tcatcgccaa cgccgcctgc gacccggagg tggtcgacgt cgccaatttt
    31741 ctgaacaagg cgggagcccg aatcacgggc gcgggcacgc cgaacgtggt gatcgagccg
    31801 gtcaggcggc tgaaggcggt ggagtacacc gtgtccggag accggctggt cgccggcacg
    31861 tttctgatgg cgggggcgat caccggtggg cgggtgaaag tcgccgggtg cgacccggag
    31921 cacctccggg tggtgctcca caagctcggg gagatgggct gcacatcgga ggccacacgc
    31981 accaccgtga ccgtcgccgg cccgaaacgg cttcagccgg tgcgggtgac gacttttccc
    32041 tatcccggtt tcccgaccga cctgcaggcc tgcattatgg cgatggcctg tattgccgag
    32101 ggaacctcgc atatccggga aactgttttt gaagacaggt tcagccatac gatggagatg
    32161 cgccgcctcg gggcggacat cagcgtgtcc agcgacgagg cggtgatcaa cggcgtttcc
    32221 gcgctgcgcg gggcgaccgt gatggcgtcg gacattcggg cgggggcggg aatcgtgttg
    32281 gcggccctgg cggccgccgg tcggtcggag atactccggg tgtatcatat cgaccggggg
    32341 tataaccgga tagaggaaaa gctcggcgct cttggcgccg atataaccag agaagcggag
    32401 taagacgccc ggttctcccg tgaccggacc gcgggccggt cgtttccccc ggcggcgccg
    32461 cggcggccca aaaccgaaaa ggtgatgccc gacatgagaa aatcccacat tctcgccccg
    32521 ttgctcgccc tggccgccgc cctggtcgtc tccgggaccg ccccggccca ggaaacgtat
    32581 ttcgggaaga acaaggtccg gtacaagaac ttcgactggg agtacatcca gacccgccac
    32641 ttcgatatcc atttctatga aggggcgtat ccgaccgcca agttcgccgc cgccgtgctc
    32701 gagtcggcct acgtggagat cacccaggag ctcggctacc acctccagga gccggtgccg
    32761 attttcgtgt acaactccca caacgatttc cagcagacca acatcatctc cagtctcatt
    32821 cccgaagggg tgggcggttt caccgaggcc ttcaagaacc gcatcgtgat cccgttcacc
    32881 ggatcctacg aggatttccg gcacgtgctc caccacgagc tgacccacgc cgtcatctac
    32941 gatctccttt tcggcaaatc cctgtcctcg ctcatctcgc gcaaccggct gttcaatctc
    33001 ccgctctggt tcgccgaggg ctatgcggag tactcctcgc ggcacggctg ggactactgg
    33061 gccgacatgg tggtgcgcga cgcgacgatc aacggctacc tggccccgcc cgactatgtc
    33121 ggcggctacc ttgcctacaa gcaggggcag gccatgatca agtacatcgc cgacaaatac
    33181 ggcgaggaca agctggggga gatcctgcgc aaggggaagg tctacctgac catggaccgg
    33241 gcgctcaaag aggcgatcgg gattgaccag gagaaactgt ggaaagagtt ttcgctcgag
    33301 atgaagcggc actactggcc ggagattgcg tcccgcaaaa atgccgatga gatcgggcgg
    33361 cagctcacca aggcgcgcaa ggacggctcg tacatgaacg aggaaccggc ctactcgccg
    33421 gagggggaca agatcgccat tttcaccgac cgctccgact acaccgagat cgtgctcatc
    33481 tcggccgcgg acgggcgccg ggtcaagacg ctggtgaaag cagagcggtc cggggacctc
    33541 gagtcgctcc attcctacgt gtcgggcatc tcgtggtcgc cggacggccg gagcatcgcg
    33601 ttcgtcgcca agtccggcgg ccgagacgcg atcttcctgt acgacgtgcg ctcgggggac
    33661 gtgaaagtga aacgggtgct cccctactac aacgtcgtct cgccggcctg gtcgccggac
    33721 ggttcccgga tcgcttttac ggcgctcaag gactacaagc gggacctgtt cgtctacacg
    33781 atcgcgaccg acgaggtgga gcagatcacg gacgatcggt ttgacgacaa ttcgccctcg
    33841 tgggtgccgg gcgcgaacgc gctgatgttt tcctccgacc ggccccaccc gcagtcgccg
    33901 gaggcgcggg ccgaggtcaa cccgtatgcc tcccccggcg ccttcatgcc gggcgacttc
    33961 gtctacgggt actacaacat cttccgggtg gacctgggcg accggaccgt ggcgcccgtc
    34021 gacgtcggcc ccggacagaa caagacgccg gaagtgtcgt ccgacggatc gcggctggcc
    34081 ttcatttcca accggaacgg gatcgacaac atctatgtcc aggtgctcgc gaccgggcag
    34141 tgcttcgcgg tcaccgacat cctgagcggg gtcaaatccc tctcgtggtc gccggacaac
    34201 gagaagatcg cgtttgaggc gttccacgaa ggggcgttcg acatcttcgt gctcgaggat
    34261 ctgcagcctt caggtgacca cggcctgctg gcggcgacgg atttcgtgaa gggggcgtac
    34321 aaccgggcgg tgaaacagcg ggccgggcag gctctggcct cgcaccagga ggcggtcgac
    34381 agcgccgcgg tggcgcgccc caacacggcg gcggccgaca ccgcgaccgc ctttgcggag
    34441 ggggagaggc gcggagactc gctcgccctg ggggcggcgc cggattcgac gcaggccgcc
    34501 gacaccgccg ccgtcccaac cggaggcgcg ccctccggcg ccaccgccgc cgccccgccc
    34561 gacacggcgg ccgccgacac ggcccgggcc gacagcgccg gcgtggtgaa gaagaccggc
    34621 gtgtacggcg gcgagtacgt gttcgtcagc gagggacgcc gcgacccgct cgattcggtt
    34681 ctcgaggagg tgtcgcccga ctcgatccgg gcgctgcggg aggggcaggt ggaaccggag
    34741 aagttcgatg agcggccggc ggccgcctcc gacggcgact acgcggtgaa gaagtacaag
    34801 gtgaaattca cgcccgatta tgtcggcggg ggagtggcct acgacaccta tttcggtctg
    34861 cgcggtcaga cctatttcat tttctccgac tacctcggca accaccagtt ctatgtcgcc
    34921 accgaactgg tgaacacgat cgaccagtcg ttcattcagg cgtactactt caacagcgtc
    34981 caccgcacga gtttcggcgg cggcgttttc cacaccaaga atttctacct cgacgccaac
    35041 gactacctct tctcggaccg gttctacggg atccagctgt tcacgaccta cccgttttcc
    35101 accttcgccc ggatgcagct ctcgggagcg cagatcttca tcgaccggga gtaccacgac
    35161 cagtatgatc tgcgcccgaa ccgcaattcg aaagtgacca ccggggagct ggcctacgtg
    35221 gccgacaaca tcgtgtgggg gatcaccggt ccggtgaacg ggacgcgggc gcgcctggcg
    35281 gtcgaggcgg gcgtcaatct cttcgatgca aacgacatcg ccttccaggc ggcgagcttc
    35341 gactaccgcc gctactggca cttccggaag acctattcga tggcgctgcg gctgaccggc
    35401 ggagcgtcgt tcggaaagac gcccaagctg tatttcctcg gggggacgac caactggatc
    35461 gggaaccgga cggtggatgc gaaggtgtac gatgtcgaga acctgtattt cctcgacgtg
    35521 gtgacgccgc tgcggggggt ggagtactac gagctctcgg gcaaccgcta cgggctggtc
    35581 aactgggagt tccgcttccc gctcatcgat tatttcgtca tgcgtttccc gctcgggctg
    35641 gcgctgacga acatccaggg cgtgatcttc accgacttcg gcgcggcgtg gacggacagc
    35701 gatttcaagt tcggcacgag cgagaacggc caccgtcggc tggcggatcc gaaagcgggg
    35761 ttcggattcg ggatgcgggc gaatctcgga ttcgtggtgc tccgctatga tctggcttgg
    35821 tcgaccgatt tcgcggaggt gtcggcgcgc tcgcgctcat acctctcgct gggtgcggat
    35881 ttctagcggt cccgatcgga cgggaatgaa aaacgcggcc gtggtccggc cgcgttttcg
    35941 ttgttgctct tttagcattg cgcttcgagc ggtgtctgtc gcgcgtgtga cagacacctg
    36001 aatagaaggg caatcacaaa ttttgtcaag ggaaaaagtg gcctgcgggt gcggcgccgc
    36061 gcgcgcggcg ccgccggatc ccccgcgcta cgtccgccgc cggggatgag ccgctctgcg
    36121 ccgcccgcgc ttccgcggcg actccttcgc gccgccggcc ctcttcttgc cggcgctgtc
    36181 ggtacggttg gaggactcgt cgtcgcggcc gaaattctgg agcgtaagcg ccatctccat
    36241 caacttctcg tcgacgatgc cgaacacggt ccctttcggg aaccggccgt tcgccccgcg
    36301 cttgccggcg gggagaccgg tgagaatctc gatcgcctcg gcgatcgtcc tgatcggcca
    36361 gatgtggaag cgccgagctt tcaccgccgc gatcacgtcg ggccggatga gaagatcgtt
    36421 gacattctgg tgggggatga tgacgccctg gtcgccggtg aggccgcggg cggcgcacac
    36481 gtcgaaccag ccttccactt tctgattcac gccgccgatc ggctggatct ccccctgctg
    36541 gttgacggaa ccggtgacgg cgatcccctg gcggataggc aaaccgccga gcgaggagag
    36601 gatggcgtac acctcggccg aggaggcgct gtcgccgtcc acgccgctgt aggcctgctc
    36661 gaacgtgatg gaggccgaga cggcgagcgg tttgtcctgt gcgaagagct ggcggaggta
    36721 gcccgacatg acgagcacgc ccttgttgtg gatcggcccg gagaggtcgg cctcgcgttc
    36781 gatgttgatg atgccggagc ggccgagcga ggtgctggcg gtgatgcggc tgggccggcc
    36841 gaacgagtag tcgccgatgt ggtagaccga cagaccgttg atccgcccga ccgcggcgcc
    36901 ggtggtgtcc accatgagca cattctggtc gaacatctcc tggatcttct cttcggtcag
    36961 gttgacccgc cggcgtttga gcgtgatcgc gcagtggacg tcgtcgcggt tggcggcggc
    37021 ggccccgcgc tggcgggcgc agtaggcggc ctcgcggata atgtcggaga tggcggagaa
    37081 gcgcgacgag agcttgtcgc gccgtccgga gaggcgcatg ccgtactcca ccacggcctg
    37141 catgcccgag aggtcgaagg gggggagcga ctcctcgtcg accaccttgc gcaggaactc
    37201 gaaataccgt ttgaaattgc tgcggtcggc ggtcacctcg gtgtcgaact cggccttgat
    37261 cttgaaaatc ttcttgaaat cctcgtcgag atgccagagg agctggtgga cgcggggctc
    37321 gccgatgagg acgactttga cgtcgatcgg gatgggctcg ggcttgatcc ccgagccggc
    37381 catcatgtag aacggatcgt aggcggcgat ctcgatctcg ccgttgcgga gggagcgctt
    37441 gaggtgggtc cacacgcccg gttcgacgag ggcgtcgagg gcgtcgagca ccaggtagcc
    37501 gccggcggcg cgcaggagcg agccgccgaa aatccgggtg aagtcggtgc gccagtagcc
    37561 gaaccggtcg acgacgcgct cgagggagcc gaagaggttc ttgtaggagg gggacttttc
    37621 gaagaccacc ggcacccgcc gggccccggc gttgtcgagg atgaggttga cggagaattc
    37681 ctcaaacggc tctcgcttgc ggtagggggg cgcctcgtcc tcgccccgcc gcgcctgcga
    37741 ctcgcggaac cgctccaggt ccgactggag cgcctccgcc acctcgtcga ggtactccac
    37801 gaccttggcc ccggggtagc ggcgcttcag caggctcact ttgtcggcga tcagcggcgc
    37861 gaccatcgag tggttgagct tctcaagcgc gtcctccatc ttgccggtca gtttcttggc
    37921 ctcgatcgtg atcgtgtcga actcgcggcg gaggctgtcc caggtccggc ggagctggtc
    37981 gagccgcgac agcgggaact tcccgtcccg cgcctggcgc tcgagctgct ccagcgagac
    38041 cgggttgtcc tcgatcagcg gctggatctc gttgcgcgtc cccagccccg actggatctg
    38101 aaccatgacg aagccgacgt tgctgaggcg ctcctcaaac gcgtggatca gttccttctg
    38161 gcggttctcg tactcccgga tgatgcggct ctggcggtcc ttgtagtcct cggacatgaa
    38221 taccttgggg acgaccttgc gcagcgactg gatcaggtag ctcatgtcct tgcggaaccg
    38281 gctgccctgc ccggcgcgga aagtgagcac ccggggacag tcttcattct tgaagttgtt
    38341 gacgtagcag atatcgggca gcttgggctc cttcgcgcgc gtgaactgct cgaggaggtg
    38401 gagagtcgca tcggtgcggc cggtgccgac gctgccggtg acgaagatgt tgtaacccgg
    38461 cgaggtcatg ttcaggccgg tgcggatcgc ttcaatcgca cgatcctgcc cgatcacgcc
    38521 gaccgcgggc ttcacatcat cggacgactt cactccctgg ggccggaagt tgacggagta
    38581 atcgagttgc cgggcggtca gttcggtgtg tttgcgggtc cgcttggtct tcggcggcat
    38641 gatccaaatc ctccacgggt atgagagttt actacctcaa tatagaattg aggggtgttg
    38701 aaacaacctc aaatgcgacg tcgctggtcc agtcacatag tacgatgtgc ccgcgcggtt
    38761 ggatgaacgg tcatggggag acaaatatcc ggttatgatc taacgacttg agttgtgcga
    38821 ccggtcattg ctgtggagaa ttcctgtagt tggctcatag ttgtctgcca caatgacaca
    38881 tggtgctgcc atttgagcga gtgtgtgatt ggtgtgtgat gcggtggcgg gtggaaaata
    38941 ggcaaaaaaa cttgtcaaat ttcgccgctg actaattagt tcggcccctt ggtattacac
    39001 gagatgattc ttgtaattcg ttgtccggca atggagtcac ttcacttgac aggggtttta
    39061 gattttctat attctgagcc gttgcttttt tagcattgcg cgttgagttt tggcggtatc
    39121 gtctaggggt taggacgtgt ggttctcagc catgaaaccg gggttcgaat ccccgtaccg
    39181 ctaagagtca ggctgtgaag gggtcaggag agtggtcacc tgaccccttc ggctattaat
    39241 cgagcactcc actcgcggtc cttcgctgtc ccctgctaca ggtcgatcgg tccgtcgccg
    39301 tcgccccgat tgatatggag tcgttcccat gcgggatcgg ccgcggcgag gcgccgggtg
    39361 agggccggcg cggtggcgag gatgaaattc gacggcccgg agaggaagcc gccgttggcg
    39421 agggccgagc accaccacgg gtgcatctgg ttggagacga tcaggtcgac atcgcgctcc
    39481 tcgaggtagt cggtggcggc ggcgatcacg gcgtcggcct gctcgggcgc ggcgaagcag
    39541 tcggcgatcg tgccgacgtg catgttgccg aagtagttgt ggtttttcat aagggtgtgg
    39601 agcacgaccg cccagccgac cggagcaccg tcgagagcga cctgcaggcg gtggatgcgc
    39661 cggtcttcgg ccggatacag caggttcagt tccggagcgc ggcggacggc ggcgaaggag
    39721 tagtggcccg cgccgctctc ccacagcccg ttggtccagt gatcgaacgc ggggacaatc
    39781 tccgcggcgg tccggccgag gcgcgcacgc ttcttcctcc gcgaggcctg cagcgagagg
    39841 atcccgatcg agccgacgcc ggtggccgcc gccacatcgc tcaggatacg ccaggcgggg
    39901 cgctcgcgca ggaggctcag gttccgcagg aacgactgag ggtggcagac gcggaaatgg
    39961 aacggcaccg ctttgagctg ccagccggtc gcggcgagca tctgcggcag cgggcggtcg
    40021 aacccgccca ggccgagcgc gtagagcacc ggccggcgcc ggagggcatc cgccagcagc
    40081 tgcgggccca ccgctgcgta ggtgcggtcg atcatcccct ccgagagcgg caactggtaa
    40141 ttgccgaccg ccacgacctc gccgccggcc cggaacggct ggtgtttgag gatatacccg
    40201 ccgcggactg cgccgccgtc gtcaacggca acgtagcatt cctggcggag gtcggaaccg
    40261 gcgcgcgggg gtagccaggc cgggaccgga ttttcgggga attgggagcg cacgcctccg
    40321 tggcgaaggc gccggttgaa ctgcgtcacc tcaaaggtgt gccggtcggt gtatggttcg
    40381 attctcacag ccatcgatat agtccgcatc gtccggcgcc ttgcgcggcg tcggcccgcc
    40441 ggggaaggcg ctggtcggcc gaacgccggc ggtcgaggtc gtcgagagcg gcatagaccg
    40501 cctcgagcgc actgtcgtgg acagtcatcg ttgtggttcc atgttatccg tccgctgggg
    40561 gcccggtttc gccgcccaat gtacgaaatg gttcgggggt tgtcacgcgg gatgtcgggg
    40621 cggcggcgcc gggcgcgggg ggcgcggtcc cgggccaagc gcagcttttg tttccagggc
    40681 cttccccggt cccggtccga agcgttctcg ttgacaaacc ccggtgaacc ggtatattcc
    40741 ggcgtgaggc gaatgcggtt acaggttccc ctgcgcgggg cggcgctgat cggcgcggcc
    40801 gttcttgccg attggctgcc ggcggcggcc caggagagcg ccgcctccac ctccgtgttt
    40861 cgcgccgacc ggatcaacgc gctgatcttc gtcgtacttt tctccaccgc catcctcctc
    40921 tacacacgct gggcgcggca gggaaaaccg ctgtttcttc ggaagatcgc cggtctcgac
    40981 gcgctggagg aggcggtcgg ccgggcgacc gagatgggca agccggtact gttcatcccg
    41041 gggatccttg agcttgacga catccagacg atcgcgagca tctctatcct cggccgggtg
    41101 gccaagatca cggcgcagta cgaaacgccg ctctacgtgc ccgttcgcta cccgctggtg
    41161 ctggcggccg ggcaggaggt ggtggagcag gcgtaccggg aagcggggcg tcccgacgcc
    41221 tacgaccggg acatggtgcg gtacgtggcg ggcgagcagt tcgccttcac cgccaacgtg
    41281 aacgggtaca tggtccggga gcacccggcg accaacatct acatgggggg gtttttcgcc
    41341 gaatcgctca tcctcgcgga gaccggcaac gcggccgggt cgatccagat tgcgggcacg
    41401 gcggaggcct cgcagctgcc tttcttcgtg gccgcgtgcg actacacgct gatgggggag
    41461 gagctctacg cggcctcggc gtacctgtcg cacgagccgg tcatgctcgg cggcctgaag
    41521 gggcaggatt ttgtgaagct gatcatcatc atcgtgatcg tcgtcggcgc ggtgctgatc
    41581 gcgctcggat tcggcgacac atttgtcggg tggttcgatt ccgtgtaggc ctcggcgccg
    41641 cggtcaccta tgagaacgac tttccccatc ttcgtggcat tcgcggtcgg cctgctctat
    41701 gtcgtgtcgt tcttcttcgc cgagtcatcg ccgatcgggc gggtgtcgga gacgctcctc
    41761 gtgtgggtgt cgatcatcgg cggattcacg ctgctgctgg gggtggtgtc gatcgtgcgg
    41821 gtgaactgga cggcggtggc ccgccggcgc ccggggtgga tctatcgtct gtgcacgctg
    41881 gtgtcggtgt ttgtcatggc tttgccggcg ctcttgccgg cctcctggtc gccgctgttc
    41941 gggcgggccg agggctcggt ctacgactgg ctgtttctct atatcgactc gccgatgatg
    42001 gcgacgatgt tcgcaatgct ggccttctac atcgcctcgg ccgcctaccg ggcgtttcgc
    42061 gcccgcaaca tcgaggccac gatccttctg ctgacggcgg tggtggtgat gctctggcgg
    42121 gtgccgatgg gggaggcgtt tttgaatctg ctgcccggcg acatccccgg atggctcaac
    42181 acgtacgtca tgcgcggggt gaacgtggcc gtccagcgcg gcattctcat cggcgcggcg
    42241 ctcggggcgg cctccatgtc gctgcgcatc atcctcggca tcgaacgcac ctacatgggg
    42301 aagggctgag ccgatggcgt ggtggcaggc aatcgacaaa ctggaccgcc gctggaccta
    42361 cctcctggtg gccatcgccg tcatcatccc gttcatcctt ccggccaagt tcccggtgag
    42421 catcacgccc gaggccaagc agctgtacaa ttatgtcgag gagctgccca acggcagcgt
    42481 agtgatgctg gtgttcgact actacccctc gaccattgcg gagtgcgagc cgatgacgcg
    42541 gacggctctg caccatctgt tccgcaagga ctgcaaggtg gtcaccctga gcaacatccc
    42601 actgggcggg ccgaccatgg cggagtcggt gacgcgggag atggcccggc tgtacgggaa
    42661 agtgtacggc gtcgatttcg tcaacctggg ctacaaggcc gactacgtcg ccgttctgcg
    42721 cggcatgggc cagtcgatca aggggatatt ccccgccgac aacagcggga cgccgctcga
    42781 ttcgctgccg ctcatgcgga aggtgaagaa ttaccgggac gtggcgttca tttttgaggt
    42841 ggcggacaac gccaccgcgg actactgggc ctcgatcgtc aacgctcagt tcggggtgcc
    42901 gatggggtgc ggcacgacgg cggtgagcgc cccgcgctac tacgccttcg tctcgtccgg
    42961 gcagtttgtc gggttgctcg gcgggatgaa gggggcggcc gagtacgaga tgctcgtggg
    43021 ccaccccggc atggcgtacc gcggcatgga cgcgcagtcg ctggtgcacc tggtcatcat
    43081 cgggctggta gtggtcggca acgtcggctt cttcgccggc cgggcgagcc gccggagggg
    43141 cgcgcgatga gcgggatgca cgttttcgaa atctggatca tcgccttctt cacgctggcg
    43201 ctgttctcct tcctctacaa ggacaacccg atctaccggt tcgccgagca catttttgcc
    43261 gggctgtcgg ccggctacta cgtggggctg atctggcaca ccgttattct ccagcagttg
    43321 tggaacccga ttttgggcgg ccgctggctg ctggtgatcc cgggtctgct cggggcgctg
    43381 atgttcgccc ggttttcgtc caagaaggcg tggatatcgc ggacgccgct ggcgtttgtg
    43441 atggcggtta cggccgggat tttcctgatc tcgcaactgc acggcctcgt gctgccgcag
    43501 gtgcaggaca ccatgctgcc caagaccgag gcggttctgg ttgattcgac ggcctacgag
    43561 gagcaggcgg accggctcgt gatcaccgac agcctcgccg cgcccggagc gccggcgacg
    43621 gccggcagcg tgacggtgag caagccggag gtcgcacgga ccgggaccga cagcgtggtg
    43681 atcagcgcca cgtttcgcga cccggcgcag ccgccggtcg agcgggtggc gttcaccctg
    43741 aagctgcggc tcccggacga caagacgcag aaggcgctgg tggtgaacgc gaccacgcgg
    43801 acgccggggg tgacgatcgc cgcgggcggc gggggcgtgt acacggccag ctatgtctac
    43861 cgtccggact ccacccgggc ccccggcccc tacgacatct catttcaggc gtaccgcgtc
    43921 gaccacgagg tcatgctcac cctgctcgtg ctgctgggtg tgatcacgac gctgatctat
    43981 ttctactttt ccaaggagca cacgggcgcg ctcggggtgg cggcccggat cggcatctgg
    44041 tttatcatga tcggcttcgg cgcgcacttc ggctataccg tcatggggcg cgtgtcgctg
    44101 ctgatcgggc gggtccagtt cctcctcgag gactggatcg ggacgctgac ttcactcttc
    44161 tgatatcacg ggttatgcga tcaagaccga gagaatcttt gttttcgcgg gcgggcgcgg
    44221 gtgtgctcgc ggcggcggtg attttcctcg cctgcagcgg ggagtacgac ggcgcaaaac
    44281 cggcgtacga cacggcgcgc aatcccggcc aattcccgcc gccggcgacg gcgctcctgg
    44341 acgacctcga ggcggggcgg ctgcgcggct acgacgagac cacagagcgg ttcgccgatc
    44401 tctacacggc ccacccggcg ctcttgggcg acccgggctg gtccggggtg attgaccggc
    44461 tcggtctccg gctgacctgg cggggggacc aactggtgcg ggaggggctc ctccagtacg
    44521 ccgcggcggc cgacctgtac cggctggccg cgcagacgcg gccgaaagac agcctgctgt
    44581 ccgagcgcgc cgagctgttc gccgcgtggc aggaggcggt cggccgcggg gcggtggacc
    44641 cggtttgtct cgcggccccg ggtcggggcg gggcggccga gaagctcgag gctctgaccg
    44701 gtttcgcgct ggccggcgcc cggcaattcc ggtttgcgga gcagtatctc gccgccgcgc
    44761 tgtttgaccg ggcgacgctc gacagcctga ggcgtgcgga agcggcgggg ccgacggcgg
    44821 ccgagcgcgc cctgcgcgcc gcgctcggcg cggggccgag tcccgccgac ccgccgctgg
    44881 cgagtttccg gcaacccgcc gtcgacctca tcgccgtccg cctcacccct gcggcggcgg
    44941 acagcttttg tctggaagcg tacttcctcc cgcgcgcgaa ggtatcggcg gatctggata
    45001 tcacctgtcg gctggagacg cccgatccgg tgctgagcgc ggagcagcag ccgcggttcc
    45061 cgtacgattt ccggccggtg cggccgagtt cgatgtgggc gcccggccgc atcgcggtcg
    45121 gtctctaccg ggggaccttc ccccatccgg tttcggcggt gctgatcggt ctgcacgaga
    45181 tgcgcgaggg gcggacggtg gcgcttccgc tcggcgacag atcgacgctc tgccgcgtcc
    45241 cggtgggggc gtcgggcggg actccggacc cgccgttgtg aggagcgccc cggggttcca
    45301 agattgtgct tgcggaagga ttggccggca tctaccatag ggcaggtttg tcatgagcaa
    45361 gaggatacgc gtcatagttc cggtcgccgg ggccggcacc cggctgcagc cgcacaccct
    45421 tacccttccc aaagccctcc tcccggtggg cgggaagccg gcgctcgcgc acgtgttgga
    45481 gccgctggtg cctctggagc ccgaggaagt ggtgtttgtc atcggccatc tcggcgatca
    45541 gatcatcgac tacgtccggg agaactaccg gatcaaggcg cgtttcgcgg cccaggagaa
    45601 gctgctcggc ctcggctatg ccctgcacct cgcgctgcgg gagatggcgg acggtccgac
    45661 gctggtgatt ctcggcgaca cggtggtcga gtgcgactac ggggcgttcc tgggggccgg
    45721 cgagtacgtc ttgggcctgc ggtcggtgga caacccgcac cgcttcggcg tcgcggaggt
    45781 tgacggcggc cggattgtcg ggctcgagga gaaaccggcc gatccccggt cgaacctcgc
    45841 gctgatcggg ctgtactact tctccgatac ggcacagctg cggaaggagc tggagtacct
    45901 ggtgcgcacg ggtaagacga cgggcgggga gattcagttg accgacgccc tccacgccat
    45961 gattgcggcc ggcgtgcggt tcgcgcccta cgaggtggcg cgctggtacg actgcgggaa
    46021 gatggagacg ctcatttctt cgaaccgcga gatcctcaag cagcgtgcga ctcccgcggc
    46081 gccggccggg tcggtgttcg tgccgccggt ttacgcggct cccaccgcga ccatcatcaa
    46141 cagcattatc ggtccgaacg tatcgatcgg cgaggccgcg cggatcgaac gttccgttgt
    46201 cagcaacgcc attatcggcg aacgatcaca gatagtgaat gccgtgctcg accacacgct
    46261 gatcggccgc gacgtgttcc tcgagggaac gccgcgccgc atgaatgtcg gcgacaagtc
    46321 gcaggtcggc gacgtgtgag cgcagcggcg gcgcaggcac agccggcttg aggtgaagga
    46381 gactgacgaa tgcccggagg caattttctc tttacatcgg aatcggtgac cgagggccac
    46441 cccgacaaaa tgtgcgacca gatttcggac ggcgtgctcg acgaggtgct ccgccgcgac
    46501 ccgcacgggc gggtcgcctg cgagacgttc gtgacggtcg gtctggtgat tgtcggcggt
    46561 gagatcacca ccgacgcctg ggtggacatc gagagactcg tccgcggggt ggtcaaacgg
    46621 atcggctatg tcgatccgac gctggggttc tcgtatgatt cctgcgccat cctcaacgcc
    46681 atcggccagc agtcgccgga catcaagcag ggcgtcgaca cgggcggggc gggcgaccag
    46741 ggcctcatgt gcggctacgc cagccgcgag acggcggagc tcatgccgat gccaatccag
    46801 ttcgcgcaca agctgtgccg ccgcctcgcc gaggtgcgca aaaaagcgat cctcccctac
    46861 ctcggccccg acggcaaggc ccaggtgacg atcgagtacg aagagggccg gccccggcgg
    46921 tgcgaggcga tcgtcgtctc cacccagcac cgggagtcga ttctcgacgc gacggggaag
    46981 aagatcaccg agcaggcgcg cgcggaaata atccagaacg tcgtcgcgcc cgtcattccc
    47041 gccgacatga tcgacgcgcg gacgaagttc tacgtcaatc cgaccggcag attcgtgatc
    47101 ggcgggccgc gctccgacac ggggatgacc gggcggaaaa tcatcgtcga cacctacggc
    47161 ggcatggcgg cgcacggcgg cggagcgttc tcgggcaagg atccgacgaa ggttgaccgc
    47221 tcggccagct acatggcccg ctacatcgcc aagaacgtgg tggcctccgg gatcgccgag
    47281 aagtgcacgc tgcaggtggc ctacgccatc ggcgtcgccg agccggtgtc gctgacagtg
    47341 ttcgccgacg ggacggcgaa gattccggaa cggcgcatcg ccgaactgat ccgcaagcac
    47401 ttcgacctca ccccccgggg aatcatcgag aagctcaatc tgctgcgtcc gatctattcg
    47461 gccaccgcgg cctacgggca tttcggccgc gaggagagcg agttcacgtg ggagcggacg
    47521 gacatggcgg ccgccctggc gaaggatgcg tgagcgatgg gcaagcggac ggacattcat
    47581 aatcccaggc tggccggccc ggggcgcgac cgcatcgagt gggccgagca gcgcatgccg
    47641 gtgctccggc tgatccggga acggttccgc aaggagcagc cgctgaaggg tctccgcctg
    47701 gcctgctgcc tgcacgtgac gaccgagacg gcgaacctcg tgcgcacgct tcaggcgggc
    47761 ggcgcgacgg tcgccctgtg cgcctcgaac cccctttcga cccaggatga cacggcggcc
    47821 tcgctcgtga aagatttcgg catcgccgtc tacgccatcc acggcgagga caccaagacc
    47881 tactaccggc acattcacca ggcgctcgac ggccggccgc acatcacgat ggacgacggg
    47941 gcggacctgg tgtcgacgct tcacagcgag cgccaggaac tcatcggcaa catcctcgcc
    48001 gggaccgagg agaccacgac cggcgtcatc cggctgcggg cgatggccca ggagaagcgg
    48061 ctcctgttcc cggtgatcgc ggtcaacgat tcccggacca aacacttctt cgacaaccgc
    48121 tacggtacgg gccagtcgac gatcgacggc gtcctgcgcg ccaccaacat gctcatcgcc
    48181 gggtccaccg tggtcatcgc cggctacggc tggtgcggcc gggggctggc gatgcgggcg
    48241 aaggggatgg gcgcgcaggt gattgtgacc gaggtcgacg cgctcaaggc gctcgaggcg
    48301 accatggacg gctacacggt gctgccgatg gcgcaggcgg cgcccaaggg ggacctcttt
    48361 atcaccgtga ccggcgacct gcacgtgatc cggctcgagc actttcgccg gatgaaggac
    48421 ggcgcgatcg tgtgcaactc ggggcacttc aatgtcgagc tcgacctggc ggcgctcgcg
    48481 aaggcggcca ggaggaaacg ccgggtgcgg ccgaatgtcg acgagtacgt gctcgaccgg
    48541 cggcgcattt atgtgctcgg cgaggggcgg ctgatcaacc tggcggcggc cgaggggcac
    48601 cccgcgatgg tgatggacat gtcgtttgcc aaccaggccc tggccgccga gtatgtggta
    48661 cacaatcacg ccctgctcga ccggtgcgtc tacactgtgc ccgaggcgat cgaccggcag
    48721 attgcggccc tgaagctgaa atcgatgggc atcgcgctcg accggcttac ccccgaacag
    48781 cgcaagtacc tggccagctg ggacatgggg acgtagggcc gccgcccgcc gagagtctcc
    48841 gcagttgccg ctccatcccg ccttctgccg gccgccgtct gcccacgctc gccggggaaa
    48901 gcaatcggcg gcatggtctt tggcgacggt tccatcgcgg tgtcgcgggg acgtgcttac
    48961 gtgcggcgcc gcggcgaaaa atgtgattgc gcggtccttc cccctgatgt atccttgtgg
    49021 aggaatattc tccatgttcg ggatttcaga aaatgccccg aaacatggca cccacaccac
    49081 acggaggagt ttatgcgcgt acgcacgctc gtgctcggcc tcgtcgcggc ggccgttctc
    49141 cccgggctcg tcctggccca gtcggacgct ccgtcgcagt cggaacaagg gatgcccccg
    49201 atggggccgc ccgaggagat gaaagagtgc gcctggctgg tcggcgactg gaccgcggac
    49261 atgaagatgc ggatggcggc cgactcgccc tgggtggcaa cgagagggtc ggcccactac
    49321 gagacggtgc tcgacggcgg ggccctgctc atgacctttg agattccgcc gtcggcggag
    49381 atgccgatgc tgttccgggg gatggggctc cagacgtggg atcgcgagac caaccagtgg
    49441 cagatgacct ggaccgacaa catgacctgc cgcaccacga tctacacggg accgagaacg
    49501 gaggggcaga cggtgatgac cggcgaggat ttgtggaacg ggatgaagat gctctcccgg
    49561 atcaccgcgt ccgacgagac tccgaccggt ttcgactggc tgatggagac ttcgctggac
    49621 ggcggcaaga gctggtacgc cagcggccag gccaagtaca cgagagtgaa atgagccggc
    49681 cggcaaaccg gcggcaagcg acggcggggc cgaacccccg ccgttttttt ttgccggccg
    49741 gtcatgcccg cacggcgtcc gcgattcttt ttgtcggtcc ccgcgcaacc tgctccggaa
    49801 actcccgtat gaatggtaca tctgcgaaag atcacctcgg gcggagaccg gcgccgcccc
    49861 acattggccg cggcgcccgg aacgagaggc tgcactcagg agagtttacg atgagattta
    49921 gagaccattt ggacggccgg atcgccgtct tcgagttgtc ggggaaaatc atgggcggcc
    49981 gcgattccac gcttttccag ggacgggtga acgagtacgt gaacatcaac cgcaaccgga
    50041 ttctcatcga catggcgggg gtggaatgga ccaacagccg cggccttggg atgttgatcg
    50101 ccgcgttgcg gctggtccag ggctcgggcg ggcgactggc gctcaccaat atcgagcgcg
    50161 tccggtcgct tctggcggtg tcgcggctgg cgaccgtgtt cgagcatttc gacagccggg
    50221 ccgacgctct cgaggcgttg cgctccgact aggcgattcc ctgccgcgcc ggaatttcgg
    50281 ccggcagggc caagggcatt tgggcggtgg ggggagtccg gcggcggtcg cagggcgccg
    50341 agcgggacgg ttccgacgcc gcgattttgt ttgtccccct ttgcggaaag gtctatattg
    50401 tcgccggcga tcggccggcg gcgcgcgctg gccggcctgc cgtataaggc acaggacaag
    50461 gaggatttga tgcccatacc gcgatgcgcc gtctgcctgg cggcggctct cctcattact
    50521 ctcaccatgg catcgtgcgg tgacgatgac gggtcgacca actcggggtt cgtggcgccc
    50581 ggtgtgcatt ggacaattcg atccaccggc accgaacggc ccctcttcga catggccaaa
    50641 ggggacacgc tgtgggtggc ggtcggcggc gccgggactg tgttgacctc gaccgacggc
    50701 cagttgtgga cgacgcagcc gaccgacctg accatagacc tgtacggtat cgcctccaac
    50761 gggcagcgct tcgtggcggt cggcggccag ggacggatcg tcacctcgac cgacggtctc
    50821 acatggacaa cggccgagtc gacgttcacc gccaggttct tcttcgatgt tgcctggtcc
    50881 ggcgaggcgt tcgtggccgt ggggcagggg gggctggtgg cggcctcggc cgacggcctc
    50941 acctgggaga tctattcgag cgcggtcgcc aacaccctcc tcggcgtgcg gtggatgggt
    51001 tcgcgcttcc tcgccgtggg ggagaaaatc atcctgcggc tcgatccgcc ggagctggac
    51061 ggactggact cgctcaccgc ggccgacgtt ctcgcccggc cgccgtacgt cctgtacgac
    51121 gcggcggtca gcgattcggg gatgctggcg gtcggccagt acggcactat tctcaaagct
    51181 gtcaacgaca ccacctgggc ggtccgcgtg tcgggactga atgcttactt gtatgccatt
    51241 gagtgggtgg gcgaccggtt cgtcgccgcc ggcgaaaacg ggataattct gacttcggcc
    51301 gatgccgccc gatgggaact gatcgcgacg ctggatgatg acatccgcgg gttgtactgg
    51361 gacggcgcgc agacgctggc tgtcggggtc gccggaacga ttctccgttc cgtggacgga
    51421 catgagtgga ctgctgtgcc ctccggcccg aacgcgatcc tctctgcggt ggcggccaac
    51481 gagtctctct acgtggcggt cggcgccgga ggggcggcgc tggtcagccg ggatgctgcg
    51541 cgctggacgc tgtggccttc cgggaccacc gagcccttgg gcgcggtggc gactgacagt
    51601 acgacgtttg tcgcggtggg ggcggccggc gtgattgcgg
//
LOCUS       DAOPOE010000010        49672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_902_length_49672_cov_6.257009, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000010 DAOPOE010000000
VERSION     DAOPOE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 49672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49672
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_902_length_49672_cov_6.257009"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PKM94_02615"
     CDS             <1..1052
                     /locus_tag="PKM94_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOD65557.1"
                     /translation="TAAVIARELGIACDGRAVTGAEIESTEDAAFDRLVQEVSVYARV
                     NPEHKLRIVRALQRGGATVAMTGDGVNDAPALKTADIGVAMGITGTDVSKEAADMVLA
                     DDNFATIVAAVEEGRAIFSNIRKFLRYLLSSNIGEVMTMFLGVVGADLLGLRSDGGGA
                     IVLPLLATQILWVNLVTDGAPALALGVDPASRGLMNRPPRPRRERVITRRMWSGILFV
                     GAVIAVGTLFAVDACLPGGLVEGAGSLRYAQTMAFTTLVLFSLFNVFNARSDERSAFR
                     GLFANRWLWGSVLLSLALQAAVIYLPFLQQTFSTVPLSLGDWLFCAAVASSVLWLREL
                     TKAAVRGRRRPANRP"
     gene            1123..2367
                     /locus_tag="PKM94_02620"
     CDS             1123..2367
                     /locus_tag="PKM94_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65558.1"
                     /translation="MDTLRLTARWALPALAVIVAGCSKSVDVEDEETDGTPPLAVANL
                     AVMASTSQSAVLRWTTPADRRDDQSPGAIAAYDLRFSFDSITDENFAAAVPVEPVPAP
                     LPAGHVQQWTVFGLTPDSGHYFALKSRDRRGNWSAVSNCPWVHCAAVRPAAFADGALE
                     QAVRSHIHKPSGDILTSDIDTIGQLVVRDAGIASLAGLEDCRSLAAVDFARNDITSLA
                     PLSGLPLLSGLYLAENNVSDLNPLAGMASLRQIQLQDNPVSDLGPLATIDSLQQLILW
                     GTPVTDFSALYELRFLSDVGFDRMNLADISFMSHLKRPQICGLAFNNITSLEPLRPLY
                     MLESLNLMQNHITDLAGLTPLTGLRELNLANNQIVDLLPLIYNTGLDSGDVIRLEGNP
                     LSDYARAIQIPTLRLRKVTVIF"
     gene            complement(2483..3172)
                     /locus_tag="PKM94_02625"
     CDS             complement(2483..3172)
                     /locus_tag="PKM94_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65559.1"
                     /translation="MKLKKGLLALGVVILAAATGSAQEPARSGAVDSRMVVIPMRSVA
                     EVTADIDNATMVKEMAAAQKAQAEGRLKEIELTIEQKQTALKDVDRRKGEAKKGQRQS
                     EVIALDIEKKVEQQAIDLLKRLKDLRKAEIEQAQVEGELAERRIGVLQLEAELQRKRI
                     EYDSLSTAGASPLSQTAAQQVLGEIEVKLLKLQQEQAGVTQKLASRQKDIVARRIKLH
                     DAQVKLGMPRA"
     gene            3387..4832
                     /locus_tag="PKM94_02630"
     CDS             3387..4832
                     /locus_tag="PKM94_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOD65560.1"
                     /translation="MDALLIAKAGRDLHLLPRMANRHGMIAGATGTGKTVTLQVMAEQ
                     FSRLGVPVFLADVKGDLAGLSQPGGGNARVDERLKALGVNDWIWQGCPVRFWDVYGEQ
                     GHPVRTTVSDMGPLLLSRLLDLNDTQAGVLSLVFRLADDNGLLLVDMKDLRAMLQYAA
                     DNSRRIQTAYGNISPASVGAIQRALLALEEQGGDRLLGEPALDIDDLRQTDGDGRGVV
                     NILAADRLLQTPRVYTTFLLWLLSELFEQLPEAGDLDKPKLVFFFDEAHLLFDEAPKV
                     LVEKIEQVVRLIRSRGVGVYFISQNPLDVPDDVLGQLGNRVQHALRAFTPKDQKAVRS
                     AAETFRANPALDVERAITELATGEALVSLLDAQGSPSPVERALIYPPHSRIGAIAPET
                     RRDLIGQSPLRGRYETLLDRESAYEMLRARADRAAADGASPSPRPGRPSSGASGNAAK
                     IATSIARSAGSQLGRELVRGVLGSLFGRRRR"
     gene            5068..5361
                     /locus_tag="PKM94_02635"
     CDS             5068..5361
                     /locus_tag="PKM94_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65561.1"
                     /translation="MGNLLSIIIQLICGAVGGNVAGSLMKKSSLGTIGNSIAGILGGG
                     IGGQILGALGVGAAGGGTDVGSIVGSILGGGVGGGLLMAIVGVIKKAMSGQKS"
     gene            5776..7011
                     /locus_tag="PKM94_02640"
     CDS             5776..7011
                     /locus_tag="PKM94_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOD65562.1"
                     /translation="MSELIDNAKARQAVLKHLILQLHEGKAPQEVKPQLLRLLGRVPY
                     HEVVAVEQELIKDGVPVEDILRLCDLHSAAMKGALDSSGPPELPPGHPVDTFRKENQA
                     LIGELELADRLFAEIEALPEEADAEEPLGHLRVRFSRLADVGKHYSRKENLVFPYLEQ
                     KGITGPPTVMWGKDDEVRAAVKGALEALAYSGRVSVGDAKGVIDLVLRPAVEAIREMI
                     FKEEEILFPMCLEKLTDAEWYAIARQSPAIGFCIYDPVEDWQPEGPAAETETASPAAP
                     DRLQFPSGSLSPVEIQTILNTIPFDLTFVGADDTVRYFTQGRERIFERNRAIIGRKVQ
                     MCHPPGSVHIVQRIIDDFKAGRADRAPFWIELHGKFIHIEYFAVRDEHGTYLGTLEVS
                     QDLTGKRALTGQKRLLSYE"
     gene            7021..7557
                     /locus_tag="PKM94_02645"
     CDS             7021..7557
                     /locus_tag="PKM94_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOD65563.1"
                     /translation="MAETPRPLITPQTKVGDLLDAYPELEPLLIEIALPFRKLRNPVL
                     RRTVARVTSLAQAARVGNVSVVELVRRLRRAAGQPDFVADVSTAPAASPGSAPPFWMD
                     PSAVIETLDARPMIDAGEQPLGAVMRALARLQAGQICEVITPFEPAPLIDKAAARGYR
                     VWTAAPAAGEFRTYFTPE"
     gene            complement(7579..9093)
                     /locus_tag="PKM94_02650"
     CDS             complement(7579..9093)
                     /locus_tag="PKM94_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOD65564.1"
                     /translation="MREVFRGRIDRLRPRRVYAAARAAGIARLYAAGIGIAFAIGLAA
                     AAALLAELATPRPDLLDNTRFGAVLTLHGTVMVFLVLIPMLPAVIGNLLVPQAVGAAQ
                     TALPRLTLAGWGAYVLGAAGVIAGAELSGYQGGWRMILPPEVGTERAYGVLAAGLAAA
                     AIATTLVNVALAWTILSRRHRTVPMAEMPLLAWFFLLGSLVQTAVVPIRLFFLLLQSS
                     AGLAESAAAWFGAADPVSFQQTLFWMYANPAILAVVLPAAGIALDLLGGAGRPVRLPR
                     RTLITAGMAVALLSLVSWGQHLLTAAEGSRMAAAGSLYGLLAIVPATVIFGLLLRAWL
                     RARLAEPALNAALFGQAAAFAALAASGSALAAPGLGVHLQDTYATVGHLHFGALGIGV
                     MAFVAGTIHARSRSGDPRCAGRAAARWIYGMTAGLLLTFLPLAVVGAQGAPRAFHEYA
                     RGTQPAHLIAAAGGAVLIVSLAGLAIHLRRGARPRETAETPAALGRADGSRAAH"
     gene            complement(9096..9887)
                     /locus_tag="PKM94_02655"
     CDS             complement(9096..9887)
                     /locus_tag="PKM94_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD65565.1"
                     /translation="MTALRNVTWFVVKDIVRSRWSLVNTLFFAAATAGLFYLQADAGK
                     AAVSLSSLCLVFVPLISLLYPAMYYYSAREFVELILTQPVGRRAVYLGLFAGIFASLW
                     LGFALGIGVPYLVLGGVNAAGMTALAAVLLVGTALGAIFTGLALVAAVRFDDRGKGLA
                     VVLGIWLTAAIGYDGAVLAAATAFAAYPLETPLLAAALGNPVDLARVLLLIHSDMAAL
                     MGYTGAVFMRFFDRASGLGLAAAVLTLWAAAPVWYGIRRFARKDF"
     gene            complement(9884..10594)
                     /locus_tag="PKM94_02660"
     CDS             complement(9884..10594)
                     /locus_tag="PKM94_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65566.1"
                     /translation="MIETRGLTKRFRRFVALRDVSFDVTPGRITGVIGPNGSGKSTLI
                     KCLVGLARPSEGVILYDGRLPDAAGQYRRRIGYMPQAATFPQDLTGREVIDLVVRLRG
                     ERPASGGALLERFGLHAHLDKRVRTLSGGTRQKLSAAVAALYDPAVLILDEPTAGLDP
                     GASRVLKEHVLERKGEGRAVLLTTHIIGDLEELADDVVLLLDGAVQFAGSVRELKLAT
                     GSPYLEAAVAQMLARGAA"
     gene            complement(10591..11892)
                     /gene="nosD"
                     /locus_tag="PKM94_02665"
     CDS             complement(10591..11892)
                     /gene="nosD"
                     /locus_tag="PKM94_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HOD65567.1"
                     /translation="MWRMRGISSKRRRGWPAGGRRFGRKLAAALGAALTVTGLASARV
                     VTVQPGGEHATVAAGVAAARAHDTVAVKAGVYREHGITIDRPLTLAGGEGAVVDADGA
                     GGIFTVTAPDVEIRNLRLRGVPTSFTAEHAAIQLEGAVRATIENNSIEACFFGIYAAN
                     SHTCRLQGNIIRGAQTRLTSSGNGIHLWYCRDITILENTIAGHRDGIYMEFVRAGTVD
                     RNRCEENLRYGLHFMFSDSCQYADNLFRRNGAGVAVMYTSNVLMENNTFEQSWGGASY
                     GVLLKDIRDSRVTGNRFCDNSTGVHMEGSDRVYVAGNLFAGNGWALKLMANCLDNTVS
                     ANDFVDNSFAVATNSRQNNSRFEGNYWSGYRGVDLDRDGYGDLPFRPVSLFSLLVESH
                     PPTLVLLRSLLVEVLDLAERAMPTLTPETLVDRRPRMRPNL"
     gene            complement(11864..12325)
                     /locus_tag="PKM94_02670"
     CDS             complement(11864..12325)
                     /locus_tag="PKM94_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOD65568.1"
                     /translation="MRVRLIPAAAVAAAVALAGCGGKGPAAMHYGEDRCDYCRMNIAD
                     PAFGTQLISGKGKVFRFDSIECLAAYELAHQADIDASARWLADVNDPGTFLAGAKAVV
                     VAGERLRSPMGLGLAATASEEAGQRLAAESGGRTVTWEEVRAYVADAWDIK"
     gene            complement(12315..13004)
                     /locus_tag="PKM94_02675"
     CDS             complement(12315..13004)
                     /locus_tag="PKM94_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65569.1"
                     /translation="MSPAILREADDRAAWRSEATGKRGRMHPAGRIALAAAALLLALT
                     YFLPLWEIALEAPQYPEGLGLEIWINRMEGQNAGDIEKINTLNHYIGMKAIHPESIKE
                     LRIMPWVMRGLMLLGLAAAAVGRRTWVLVWLVLFAAVAAGGMIDFYRWGYDYGHNLDT
                     EQAIIKVPGMTYQPPLIGSKRLLNFEAHSWPGPAGWVALGVFAVGTAVYGAEVIRARR
                     KGISGEAGDAG"
     gene            complement(13036..14958)
                     /gene="nosZ"
                     /locus_tag="PKM94_02680"
     CDS             complement(13036..14958)
                     /gene="nosZ"
                     /locus_tag="PKM94_02680"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HOD65570.1"
                     /translation="MVRRLSGKILSGLAGLVALVLLGGCGTGGNEGVAGTGDAAARVY
                     VAPGTYDEFYAFMSGGFSGQVSVYGLPSGRLLRHVPVFSQYAENGYGYSEETKNMLMT
                     SFGFVPWDDAHHPQLSQTDGVPDGRWLFINGNNTPRVARLDLTTFETTEIIELPHSGG
                     NHASPFLTPNTEYVMAGTRFSVPIPQQDVPVSTYKENFKGTLSFIKVDSATGRMSLAF
                     QILVPGFDYDLSHAGKGPSHGWAFFSTYNTEQANTLLEVNASRNDKDYMAAVNWKLAE
                     RYAAEGKAKDVPASYRHNFLDEARVAQSEVVGSVKILDPRDCPGMIYYIPTPKSPHGA
                     DVDPTGEYIVGGGKLATVIPVHSFSRMIKAIENREFEGEVDGIPVLKYEAVLAGEVQN
                     PGLGPLHTEFDGQGYAYTSMFISSEIVKWKLGTWEVVDRAPAYYSIGHLMIPGGDSRA
                     PFGKYVVALNKITKDRYLPTGPELSQSAQLYDITGDKMKLLLDFPTIGEPHYAQALPA
                     ALIKDKSVKYFPIAENRNPHAITAENQARIERSGKLVRVYMSTIRTHFAPDNIEGIKA
                     GDSVLFHVTNLEQDWDAPHGFAVMGANNAELLIMPGETRTLAWKPQAPGVYPFYCTDF
                     CSALHQEMQGYIRVSK"
     gene            complement(15012..15545)
                     /locus_tag="PKM94_02685"
     CDS             complement(15012..15545)
                     /locus_tag="PKM94_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOD65571.1"
                     /translation="MTVEFRKLIGVAGFALTLWLMGCSSGPETEAERQTRWQQGKLTG
                     WELEHGIGPITDALEIGPVDAARAAQGRELFIAKCATCHYLDDRKTGPGLRDVTKRRS
                     PEYVLNQVLNPEQMGKLHPEGKKLVAQYAQFMTIQGITPDDARALLDFLRSEADKPPV
                     PLEQQPGANVPPPPPAN"
     gene            complement(15678..16112)
                     /locus_tag="PKM94_02690"
     CDS             complement(15678..16112)
                     /locus_tag="PKM94_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD65572.1"
                     /translation="MLFSKACSYAIRAAVLAAAKDPDGRRAFIPIRELADEVGVSFHF
                     LTKILRKLTEVEIMESFRGPNGGVGLVRPARQIRVIEVIAAIDGMGLFEGCALGLPQC
                     SDKTPCPLHEAWRKRREDLRAMLTRTTLADLVPKGSAEGKRR"
     gene            complement(16465..16875)
                     /locus_tag="PKM94_02695"
     CDS             complement(16465..16875)
                     /locus_tag="PKM94_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65573.1"
                     /translation="MNKRTGLLLTLALTALALLSCGGSRTLVLYEGERRPAEQVAVLD
                     VSHKSLYVMGIDTLVHRYRALSEEHIEVLPGEHTLVLNYSSWKGTSDEPMTLSFEAQA
                     GHLYQVKASAGYRRWTAWIIDMNDGSVVAGSPPD"
     gene            complement(17230..18768)
                     /gene="malQ"
                     /locus_tag="PKM94_02700"
     CDS             complement(17230..18768)
                     /gene="malQ"
                     /locus_tag="PKM94_02700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOD65574.1"
                     /translation="MTEYRRTSGILLPITALPSVYGIGDFGPGASRFLDFLARARQGV
                     WQVLPLNPTDPAHANSPYSSSSSFAGNPLLISPDRMVEDGFLGAEELQPIPAAPDDRA
                     DYEQATAYKTRLLDRAWQAFRDRPHQEEFREFCREEAGWLEDYALFVVIKRAQQGADW
                     SRWPVGLRDRNPRALDEVRKVHADELGREQFAQWLFFRQWLALKREANARGIRMFGDI
                     PIYVNYDSADTWRDTHLFKLNGGKRPEVVAGVPPDYFSTIGQLWGNPVYDWEALKREG
                     YRWWIDRLAHVFRLYDIVRIDHFRGLVAYWEVPAGHKTAQKGKWREVPTDDFLQTLQR
                     HFGRLPVVAEDLGLITDDVTATMKRYGFPGMKVLLFAFGADDPTHPYLPRNYDENAVV
                     YTGTHDNNTVQGWFEEEAKPAEKKRLFAHLSRAVPRDRVHWAMIELALQSRARLAVIP
                     MQDVLGLPGSARMNTPSTRNGNWTWRLTAQQLAAAPAAQLAELVATSDREPEGQAVPG
                     STPA"
     gene            complement(19234..19449)
                     /locus_tag="PKM94_02705"
     CDS             complement(19234..19449)
                     /locus_tag="PKM94_02705"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOD65575.1"
                     /translation="MKEQIGATAGAIWQVLQKKDKLALSQVPRAVKEKESLAFQAVGW
                     LARENKIEFQVEGKTTYVTLAPTERRA"
     gene            complement(19560..21494)
                     /gene="glgB"
                     /locus_tag="PKM94_02710"
     CDS             complement(19560..21494)
                     /gene="glgB"
                     /locus_tag="PKM94_02710"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOD65576.1"
                     /translation="MTARETSVIHGPTRLTEHDVYLFKEGSHYQLYDKLGAHPMEVDG
                     IKGTYFAVWAPNAEAISVIGDFNGWNRTAHPLGVRWDGSGIWEGFIPRLGDGAIYKYF
                     IHSRHNGYTVEKGDPFAFECELAPRTASVVRNLAYTWNDRAWMHGRRRRNALEAPIAV
                     YEVHLGSWRRVPEEGNRFLTYRELAPLLAEYVARMGFTHVELLPVMEHPFYGSWGYQT
                     VGYFAPTSRYGSPQDFMYLVDYLHQNDIGVILDIVPSHFPSDEHGLVYFDGTHLYEHE
                     DPRKGFHPDWKSYIFNYGRNEVRNFLISSSLFWLDKYHVDGLRVDAVASMLYLDYSRN
                     EGEWIPNQYGGRENLEAISFLKRMNEVVYERYPDVQMVAEESTAWPMVTRPTWLGGLG
                     FGLKWNMGWMHDTLLYMSKDPIHRKYHHDTLTFSLLYAFHENFQLPLSHDEVVHGKGS
                     LLGKMSGDTWQKQANLRLMLGYMWMHPGKKLLFMGGEFGQWDEWHHEESLSWHLTEFP
                     KHRGIQRWVEDLNRFYRGTPALYEVDFNWEGFQWVDGSDYANSVISFLRRDRQGRASI
                     LVVCNFTPVVRSGYLIGAPYPGYWQEVLNSDAQEYGGGGQGNAGGCDTRPVPMHGFPQ
                     SLALNLPPLAVVVLEKQDLD"
     gene            complement(21727..22290)
                     /locus_tag="PKM94_02715"
     CDS             complement(21727..22290)
                     /locus_tag="PKM94_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOD65577.1"
                     /translation="MPFAVSLFLDKEASAIVTDVWRRLADMEVSESMIKANFRPHITL
                     GICEDLRVEPFRRFLAGFAGTHMVFPIILSSLGVFPLDTKGAVFFGVTPSPQLLRVHD
                     DFTRSFAEFGEGIRTYYRPGHWVPHCTLADGIGCEVVPRAMEICQQTRLPVYCLAREI
                     GVLQFPPGHELLAYSLRSDVDGFLAGT"
     gene            complement(22304..23023)
                     /locus_tag="PKM94_02720"
     CDS             complement(22304..23023)
                     /locus_tag="PKM94_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOD65578.1"
                     /translation="MKIIVFTDTHANLPALRAFRRAIEREGYDLAVHTGDAVDIGPYP
                     AESLAELLSIPRVECVMGNHDAMFVHGIPDPLAGVIDGNEAEHCAWTARQLGEDFRER
                     LRHWPYVIPHEFNGLRVTFVHYGLSATLDGFVPAAIADPTPADLDRIFGAFDTDLLFY
                     GHSHPFSDVQGRARYINPGALGCFCRPLARYCVLEFRPGGLGVTYKAAPYDDSVLRAE
                     YARRRVPAARFILKEFFGKNL"
     gene            23167..24546
                     /locus_tag="PKM94_02725"
     CDS             23167..24546
                     /locus_tag="PKM94_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOD65579.1"
                     /translation="MEAFKYHVYICNQEKPEGVPCCRARGGEKLIDALRREVAAQGLG
                     DDVHITTCGSLGLCAWGPNMIVYPEGVWYTGVGAEDVPEIVREHFGRGRPVERLARRD
                     AAAVRAEIETNRAKMLAGLKARDAAGVLPDELMADFRGFQTSRMLLTAVELDLFTAVG
                     GSASAGEIAARLEVDERGAEQLLDALTAYGLLEKHGRRYANSAAARRYLAAGGPDDSR
                     LSLLHLVGLWNRWHTLTECIRKGTSVTYTEAKDRDPGWTEAFIAAMHKNAALRADQVV
                     GALGVAGFERMLDVGGGSGAYAISFAKANPRLRAEVFDLEPVTAIARRHIDRAGLAER
                     VTTRVGDLHTTEFGSPFDLVFISAICHMNSPEENRQLCHKAFAALRPGGTVAIQDFIL
                     NADKTGPFTAALFSLNMLVGTPAGASYSISEYTEWLTGAGFADLRVVALPGPTGLVVA
                     CRPEVGPHG"
     gene            24539..25963
                     /locus_tag="PKM94_02730"
     CDS             24539..25963
                     /locus_tag="PKM94_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOD65580.1"
                     /translation="MDDAEFRRRAHELVDWMADYLANVRAYPVKSPAAPGDIRRQLPD
                     LPPETPEAFDDIMADFRRIIMPGITHWQHPRFMGYFPANSSPASVLAEMLTATLGVQG
                     MLWQTSPAAAELEEQMMLWLGAMIGIPAGWDGVIQDTASTATLTSILTAREQASDYGV
                     NQTGLQDRPRYAVYGSSETHSSIEKAVKIAGLGRENLRKVAVDAGFALRPEVLEEAVC
                     RDEDSGIRPLCAVATLGTTGSTAVDPLRRIGEICRRHRLWLHVDAAYAGTALVLPEMR
                     WMIDGIEEADTFVFNPHKWMFTNFDCSAYFVKDRGALIRTFEILPEYLKTRETAAVNN
                     YRDWGIPLGRRFRALKLWFVIRTYGLSGIREKLRLQIGLARGVADRIMESDDFELLAP
                     APLNVLCFRYRPTGAADSEALNRLNERLLEEVNRTGRVFITHTKLNGAFTLRMVIGQT
                     QVTADDVDLAWETIAGTARALGRG"
     gene            25996..27369
                     /locus_tag="PKM94_02735"
     CDS             25996..27369
                     /locus_tag="PKM94_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HOD65581.1"
                     /translation="MADQSFPENAPEAPAVSAIPLPLAPVADAERVPSLDVLRGFAVL
                     GILAINIQQMGLPQAALLNPHLQGEAGLLHTIAWGGVIVLFLQKFMTIFSLLFGAGLV
                     VMTQRAQAAGGDAASLHYRRMGWLLVIGLLHAYLLWWGDILFSYALCGMLVYLLRRLS
                     PRRLLIIGALVFLAPAPFAAGFSGLIGHLQSKGEAASLKLEAGETLSSDERASLDAWT
                     NVAVALSPAPDQISKEVDAHLGSYGGLFAQRALTALLFQTLILVLFAGWRICGLMLIG
                     MALMKWRVFAAERSKAFYLRLALFGLGIGLPLDLFGASLLVSRDFGAAVQMLVSGTVN
                     YTASAGSALGYIGIVMLLCRSGAARRLRTGLEAVGRTALSNYLLHTVVFTTVFYGYGL
                     GLFGKLGLIPLLALMTLMWLVQLSVSPRWLRHFRFGPIEWLWRSLAYRKRQPFRRVPI
                     VTARAAG"
     gene            complement(27416..28171)
                     /locus_tag="PKM94_02740"
     CDS             complement(27416..28171)
                     /locus_tag="PKM94_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD65582.1"
                     /translation="MIEMKDRVTLITGGSRGIGRAAAELFARAGSHVIINYRRDRAAA
                     LEVQAACARHGVKAAAIQADVADKAAVDRMVAETIDRFGRIDAAVNNAGVWLHHPIDE
                     MTEAQLRETVDINLLGTFHVCMAVTPHMKRQRSGVIINIASTAGQRGEAFHSPYAATK
                     GAVISLTKSLAPELVEYNIRVNCVAPGWVYTDMSIPSLGGAEGEKVLAAIPMRRVAQP
                     EEVAGPILFMASDLATFITGEILNVNGGAVLCG"
     gene            28516..29391
                     /locus_tag="PKM94_02745"
     CDS             28516..29391
                     /locus_tag="PKM94_02745"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD65583.1"
                     /translation="MKRLLLLLGLCGSLMLLFGCSQDTSVTGPSGDQSQLGQLVQRPE
                     FVDHRTDAQIALTLKNAPTNWSAYKPPKPPPDTGGDPNPNPAHKYAYVVGISDYEGTA
                     NDLNYCDDDARSMAQYFQSQGFTVHSDIDRSATAAAIEANLTWLMNAAVAGDEIAFYY
                     SGHGTTYQGYGSCLISTDLYYLTWGWVMQFINGANCTKKMIPMDACKLGSFHTGVPSG
                     MIMATASTSGYSYDAPDLGHGAWTYYFLEATETMVYGEEICTYANSGMKAWAALYRLK
                     VAPKMTDAYSGKLDI"
     gene            complement(29471..30667)
                     /locus_tag="PKM94_02750"
     CDS             complement(29471..30667)
                     /locus_tag="PKM94_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD65584.1"
                     /translation="MDDLIYLDNAATSWPKPDNVYKFMVDFYRSCGVNPGRSGFDKAI
                     EAGNILEDLRKRLTAFFGGDPETPQRLCFGYNATDALNLIIQGLLAPGDHVVTTNLEH
                     NSVIRPVNHLVRDHGVEATFVEFNAEGFVEPDAIREAIRANTRLVIVNHGSNVIGTIQ
                     PVAEIGRVCKDAGVLLAIDVSQTAGVVPIDMRRMNVDVLAFTGHKGLMGSTGIGGMCV
                     RSHVDIRHTRSGGTGVRSAYPYHLDEYPYRMEYGTPNMVGVASLWAGQDWIAEQGGVE
                     AIHAREMKLAQKLVDGFREIEGVTVYCGASLENHLSTVSMNVEGFEAGDVGIMLDVDF
                     GIATRTGLHCAPLVHKQLGTMARHGSVRFAIGPFNTEAHIDRAIEAVSEIAVQARARL
                     VQPGRG"
     gene            complement(30813..31358)
                     /locus_tag="PKM94_02755"
     CDS             complement(30813..31358)
                     /locus_tag="PKM94_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOD65585.1"
                     /translation="MDTHEASGKILVTGRPGVGKTTLIMRLAERLADLHPAGFVTEEI
                     RERGVRVGFALATLDGRQRSRLATVDVSSPYRVGKYGVDIAALERFLAAMEFPHDLHT
                     PVLIDEIGKMECLSPAFRSLVTTLLDSSRPLVATVALAGSAFIAGVKAHPAVRVWTVT
                     PEARDAQLDRLEEAVRRSCSG"
     gene            31574..33712
                     /locus_tag="PKM94_02760"
     CDS             31574..33712
                     /locus_tag="PKM94_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD65586.1"
                     /translation="MALDLVLRMLFLRLAETAGHRTERPDRFTEVLYGSAEVRGPLSV
                     VRRLLPLPTQVACPGAPPVSDEALRPLLGETGFLDRYAFSAQEDPGHDSVILGPAVLA
                     ELIETLAPGRRDRGAFFTPPEVVDFVVRAALMEWLGDHPELIIDSDGSRIPPARRAEI
                     IGRLADLTVLDPACGGGAFLLGTQRILRSLGSALDAPELPTARRLYGADIDPQAVGVA
                     RLRLWLSSPRPTPGPDVDLLISQAVVADTLSADPPYRDRRCWHLVLTNPPYGVKVPPA
                     TRDRIIDPRREGAQSRDAYGLFLANAPRLLTEGGVAGLVVADTWRTIASHLPLRRRLL
                     AQVQIRRIVDLPAGTFKATVGAEIVILRNAPPDPRTPLQAVDLRAGAETGLAERLRDL
                     LRAPHPSGAPAATGRYVYPQSRCRAHRRHPVFIASPALFRFLMDPSMMRIGEIADVRH
                     GLTTGDTRRFIYRRPGAPGRYPIADPERIVEGEDLAEWLTEDERQNGFNPDRLDGRYL
                     VPCDKGGASRTADGWLPCYQVPTDYYIDWRAESVAAMKRLPGFRHDGREYYFRRGVTF
                     SHTGQYAPTFRFGAGGVFDTAGSTLFPRGHDLRLLLALLNSSMVRYLFKNYLNHSVNA
                     AENPLKEIPLPRPDAGAERDLILLVDRIAARQRTDRYYPFHRHEQREIDALVFGLYGL
                     AEEEIEEVERWCVRRYPALQGPRRRARSRQ"
     gene            33969..35333
                     /locus_tag="PKM94_02765"
     CDS             33969..35333
                     /locus_tag="PKM94_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65587.1"
                     /translation="MNKLHFVLLMILATAGVTPAAVPTQVSFQGVLTNASGNPVADGS
                     YGVVFRIYATDVTPVALWTEGQTVTTSGGRFTARLGSVTPLTASIFQSSSRYLGLTVG
                     ADPEISPRTRFLTVPYAFSVATVDSAAGGQITSGVGIGVASGPYRLHVNSSDSVVSRI
                     TGDFDGPSGAIFKAEYLGPTSSDYDAYAVRGLALQNTSSEYGVGGRFEGGYNGVVGVG
                     YSAGVIGTGADANGSCMGVSGSTVSTNIGDAYGVRGTASVTGDANGYGVYGIVTGTTT
                     GVRYAGYFAGNVHVNGTLSKAAGSFKIDHPLDPANKYLYHSFVESPDMMNVYNGNVVL
                     DGAGEATVSLPDYFEVLNRDFRYQLTAIGAPAPNLHVAEKINGGRFRIAGGAPGMEVS
                     WMVTGIRQDAFANAHRIPVEENKGAEERGRYLYPEALGKTAAEGIDSARERAVDQRGD
                     QAED"
     gene            complement(35417..36760)
                     /gene="nhaA"
                     /locus_tag="PKM94_02770"
     CDS             complement(35417..36760)
                     /gene="nhaA"
                     /locus_tag="PKM94_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOD65588.1"
                     /translation="MRETTDRPLSRLTRPLARFLHTESSSGIVLLVCTAAAVAAANSP
                     LADSWMRFWNTVLTVGAGRFSLSYPLWYWVNDGLMTVFFFLVGLEIKRELVSGELREA
                     RRVIAPTAAAVGGAAAPAILFFALQHGQPGQSGWAIPMATDIAFVVGLLALLGDRVPR
                     SLKVFLLTLAIIDDLIAVLVIALFYSSAIKFGWLLGALAGFGVVAAMKQAGVRAVAAY
                     GLAGVGVWLCTLKSGIHPTVSGAALGFLTPARPLLSAESLAAMFDRIRNRLRGSPEGP
                     ELHDALEEAAFASREAVSPLERLETAVHPWSAFLIMPVFALANAGVPFAIESLTSPIA
                     SAIAVGLVLGKPAGVLAALGLVTLLRVAEPPSGVGWGALIGAGCLAGIGFTMSLFVAS
                     LSLEGQLLIDAKSGILIASTLSAVFGFTALRLTLRRPAQSSRALGQRAHEADSPS"
     gene            complement(36947..37546)
                     /locus_tag="PKM94_02775"
     CDS             complement(36947..37546)
                     /locus_tag="PKM94_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD65589.1"
                     /translation="MKKALITVAALLLMSAGVSAQVENPISLYVGGAISIPNSPEEFS
                     EAYNTGFHGSVGLGLAVMPSLQVVGKVEYHRFGLDVTQDMEDMGVTGGHNNMWMYGAD
                     ARYSFGLPAAPIKPFLLGGVGFARISASDLEGTGDLVAGLNELNPDAETNLYLNLGGG
                     VELKTGPMFSFFGQVRYVTVDTDGESSSFIPVTLGLKFF"
     gene            complement(37787..38545)
                     /locus_tag="PKM94_02780"
     CDS             complement(37787..38545)
                     /locus_tag="PKM94_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOD65590.1"
                     /translation="MSFRSAANRFLASRGLVVERSMPAVLASHTRLALDFDFLVRAQI
                     DGRAGSFRFVQIGANDGVSRPDDLIAYVREFQAAGVVVEPQPDVFASLAHNFAAYPGV
                     ALLNKAVHRERKSMTLYRFAPDVLRQRSDLPLWATTNGMASFSRRHVLNHARKLRLGP
                     EAVQELAVECVSLDEVLALVDGPPDVLKIDVEGYDFELLDSLDLARHHPRIIRFENLH
                     MTGAFYAAAIRKLSAAGYRFVANRMDTTAYATLP"
     gene            complement(38688..39557)
                     /locus_tag="PKM94_02785"
     CDS             complement(38688..39557)
                     /locus_tag="PKM94_02785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD65591.1"
                     /translation="MDLSYCEDYWDAPELKEEYRRFLAGLFGLDLSLWDRLGFWDARY
                     RPFSYFHGGTLVSNVCVYSLDMIVRGTRRRVAQISAVGTLPEFRRRGLSRQLLQTAVA
                     WARRDHDFFFLFADAEAFPLYASCGFRPAAEYKARVFLPGLRARPGAVPLDPQDPEHL
                     ALLYRLAAARTPVSDELGVLNARLFMYHCLYGLRRYILYLPDLGAAVLARRAGGILTV
                     FDIVAPSMRPFEDIYRYLGAADGETVEFLFMTDKLHLGDVDLVEVEGNGTHVMGDLPF
                     AGAPFIFPYTSQA"
     gene            complement(39610..42396)
                     /locus_tag="PKM94_02790"
     CDS             complement(39610..42396)
                     /locus_tag="PKM94_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD65592.1"
                     /translation="MYDDSTLPAVRSAEDLRRLVEGEFEADYEKLLAALSRQLNTLLG
                     TDLRAAEEFLRRIEPLYEHMPAKHRPHIVALDARLSHHRGRYRAALARYREADRRYRR
                     ERNYLASARLGQGMTEVLMYLGRYRSALAAGRRSLGYFRRRGMAAAAAGVLVNMGNVY
                     HRLDQNRLALVYYDKARAIFETRGGAPLATVEYNRANIYANRNEPHLAAPLYRRAAEI
                     YRASGLEVLYRKAQYSLAYLLFLGDRFTEALREFENVYVAFERLGDTRSMAVTRLDLV
                     ELNIHLNQFGTAIAAGEEVIAQFRRLGLRYEQAKANYFVALSRLSLGDFRLAAEALNE
                     AARLFRRERNDLWLGMVLLARSKLSSQLGRSGAALRAAAESRRLFTRSGDRRRGTDAE
                     IVHLEALCAAGRRKEAAALARRLLRRPLVSYQSYLVQTAVGRLRRAGGDAAGALDAYR
                     RAIDMVETMLAGLYPDEIRFFFVADKLTAYAGAVECLLQMDRPDESFRANLRALEVLN
                     RRYPLAAEVHARVPAALLAERERLRATLKRFGRFPEKTHRGSDSLAQQSAVEQRLWSA
                     EQRIRAHLYPARGRGRAAGPREDVQERLGGDDLLVSFVAPPGGPPGAFRATRGTVDFI
                     PLAIDAERLRRQVWELYFVLERAVISGRGERRFPGAADFYLADLHETLVAPLLRDHDQ
                     SRVIILTDGIFAQVPFGALLDRAHLPLCRRRDLRLIVNPDDLARDGVPPGARAGRGRV
                     PFAARRNAVFALLSDRLPAVELEAGAVRREFEAAGLYVDERADSTAFKEALGVCDGFI
                     HIATHAARSSENPLFSRVVLADGPFFPFDLFAAGIRADLVALSGCQTAATGLYYGNSF
                     SLAKAFYQAGARYVLATLWPVSDQVSHAFMSRFYARLRATDDVVASYRTAQAEIAATA
                     IDPALWGAFVLLGM"
     gene            42561..43142
                     /locus_tag="PKM94_02795"
     CDS             42561..43142
                     /locus_tag="PKM94_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65593.1"
                     /translation="MADGKRASVEELVGACREGAEWAWRELLGRVSPLVLALCRDSRL
                     SAEESLDIFGQVCYLLVRNIDRIRNPRRTYAFVATITRRQIYNRFHEDRGLEFVDTDR
                     LGETVEDPAGGPDRKFEETEQRLLLTEAIAMLPPRDYELLTALFFDEDKPSYEEIGAR
                     LGMPVPSIGPTRARALQRLERLLVRMGYKFDVF"
     gene            43182..43760
                     /locus_tag="PKM94_02800"
     CDS             43182..43760
                     /locus_tag="PKM94_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD65594.1"
                     /translation="MSSKRDKVERLQQYLDGRLDPEEAVRLEEHLAADPRLRRMLDLL
                     RAMRRTQPGDGALESAARGLIDRVVTDLHRRKDSPHRGLLTFDSGLLPLPAGIRPATL
                     DSRRLRYAAEGLRLELSVYPASPGTFEVIGQIEGSRRGAALEVSLRQGRQTLRERTNP
                     VHVFHFARVPEGAHDMTVEDPDGGRFEFLLEL"
     gene            44016..45407
                     /locus_tag="PKM94_02805"
     CDS             44016..45407
                     /locus_tag="PKM94_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD65595.1"
                     /translation="MTTRNRLLAFVLLLLVGSACPRGAAVAEALPGQALVRVDPDQNL
                     GCILTGTGIVIADSIPGSRLYLVRFSPTVSFDTKRQDLAGRPGISAVWANEPMHLVET
                     HQMSIGVPEDEVPVFVRGVEPQNYYAQPAAYSIGLDSALLVSRGAGVRIAVIDGGVDC
                     SHPLFADLNDSLARDFVDNDADPSEGPGTLRGHGTFVAGLIRLAAPEAELMVVRAFTG
                     DGVSTTFQVAQALDWAVSHGAGVVNMSFGTAADNGVLAQACARAVQAGAVLAASVGND
                     GIVTPMYPASYPYVLAVTAIDTLDLAASFSNGSPEADVCAPGVDIYSALPAPYYWGTW
                     SGTSFSTPLVTAVAGLVRAVRPTLTPQEMETHIRSTAARELAWGTVAPPDLFYGYGRL
                     DAFTAVLLFRLGDMDNSGVVDVVDLNTVTAMATTGATPPNSIRRQGDINSDGTVDAED
                     VVELSDELFGGDQ"
     gene            45998..47110
                     /locus_tag="PKM94_02810"
     CDS             45998..47110
                     /locus_tag="PKM94_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD65596.1"
                     /translation="MKKRLIGRALVSTAVLMLPVLTSAQGAPPTLVVTDSVRQMEFHD
                     QISLTGRTEAWVTSRVVAEVSGRVEAIAAGEGAAVQKDQTLVMIDGERMGLALQAKAA
                     EAEQARVRAALAADNLRRAEDLFSRTLISTAAIDSARAGATMAEQSHRQLEAERRRLE
                     VEHDNCTIRAPFSGYTGRKLVDVGEWVSPGTPVFELVDVSTIRVRVDLPERYFGLLSV
                     GSPATIGLSERGGEAVAGRVVGISPNASEDTHTFPVIVEVPNREGQLAGGMLVRATLS
                     LDNKFTGLAVSKDAIIRQGAQTLVYTVHEGKAAPIPVVTTSTNGKMVAVRSEALRAGT
                     PVVVRGNERIYPGAAVMIADGSRQANPPKAAPQTNQ"
     gene            47141..>49672
                     /locus_tag="PKM94_02815"
     CDS             47141..>49672
                     /locus_tag="PKM94_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD65597.1"
                     /translation="MSLITSSIRFPVTVIVGVLIVLIGGIVALTRVPVQLTPEVERPV
                     VTVTTTWVGASPGEIEKEIIEKQEEYLKSVEGLLEMSSESQDSRGTITLEFPAGTDLT
                     GAVIRVTNKLNEVPSYPATADRPVVTTSGQLEGAIAWFAVKPDSAGVYVPHQQAVIED
                     MVKPRLERVEGVASINIFGGLERELHVTFDPDRLASTGIAIDQLAEALRADNRDISGG
                     DFAEGKRRYVVRTISRYETPEQVEQTVVTVRNGVPIRVGDLAGVELTYQKPAAMVRHL
                     GVPSIAFNAQRRVGANVMEVMDGLMAQIDIINREILHPRGMHIENVYRETVYIDSAID
                     LVLSNIYVGGLLAILTLFIFLRSASAVLVIALSIPISIISTFLAMALFGRTMNVISLA
                     GMAFAVGMVVDSAIVVLENIYRHMQLGKSRWRAASDAAREVWGALLASTVTTVAVFIP
                     IMFVKEQAGQLFQDIAIAISTAIIVSLAVSVTVIPSLSARMLRVSTKLTAGDETQGWL
                     ARLAARVASFVDYVNARGRRRLVTIGAILTVAMGLTVALIPPSEYLPTGNQNLVFGVM
                     LPPPGYNLDEMVQAGQTIEAQIRPLWEADAETADTLPGGGIDNFFFVALPNQAFMGMR
                     GRAPQRGAELVPVANQALWSIPGAIGFAAQASLFGGGFAGTRSVRIDVTGPDLPKVLQ
                     IAQQVFVQVPQVLPGANARPIPGLDLGNPEVRIYPDRVRAADVGFDASAIGRSVNALV
                     DGAIVSEYFLDGREIDLLVKGRDDWSRHTQDIALLPLATPTGRVVTVGDVAEVRQEQG
                     PVQINHVERQRTVSIETVLPDGIALEDAVRRLEEQVVQPMRAQGQI"
BASE COUNT         8364 a        17268 c        15624 g         8416 t
ORIGIN      
        1 gaaccgccgc cgtgatcgcc cgcgagctcg gcatcgcgtg cgacggccgc gccgtcacgg
       61 gcgccgaaat cgagagcacc gaggacgcgg ctttcgaccg cctcgttcag gaagtctccg
      121 tctatgcccg cgtcaacccc gaacacaagc tgcgcattgt ccgggccctc cagcgcggcg
      181 gcgccaccgt ggccatgacc ggcgacggcg tcaacgatgc ccccgctctc aaaaccgccg
      241 acatcggcgt cgccatgggc attaccggca ccgacgtctc gaaagaagcc gccgacatgg
      301 tgctggccga cgacaacttc gccaccatcg tcgccgcggt cgaggagggc cgcgccatct
      361 tctccaacat ccgcaaattc ctccgctacc tcctgtcgtc gaatatcggc gaagtcatga
      421 ccatgttcct cggcgtggtg ggcgccgacc tcctcggcct caggtcggac ggcggcggcg
      481 cgatcgtgct gcccctgctg gccacgcaga tcctgtgggt caacctggtt accgacggcg
      541 cgcccgctct cgccctcggc gtcgatccgg cgagccgcgg cctcatgaac cgtccgcccc
      601 gcccccgccg cgagcgggtc atcacccgcc gcatgtggtc agggatcctc ttcgttggcg
      661 ccgtcatcgc cgtcggcacg ctcttcgccg tcgacgcctg cctccccggc gggctggtcg
      721 aaggcgccgg cagtctccgc tacgctcaga ccatggcctt caccaccctc gtcctcttct
      781 cgctgttcaa cgtcttcaac gcccggtcgg acgagcgcag cgccttccgc gggctgtttg
      841 ccaaccgctg gctctggggg tcggtcctgc tgtcgctcgc cctgcaggca gccgttatct
      901 acctgccttt tctgcagcag accttctcga ccgtgccgtt gtcgctgggc gactggctct
      961 tctgcgccgc ggtcgccagt tccgtgctgt ggctgcgcga actgaccaag gctgccgtgc
     1021 gcggccgccg gcggcctgcg aatcgccctt gacaccgcgt ccgcccggcc ctactctgga
     1081 cggagacaaa tcactctttg ttcggacacc ggaaccatgc tgatggacac ccttcgctta
     1141 accgcccgct gggcgttgcc ggcgctcgcc gtgatcgtcg ccggctgcag caaatctgtc
     1201 gacgttgaag acgaggaaac agacggcacg ccgccgctcg ccgtggccaa tttggccgtg
     1261 atggcgtcga cctcccagag cgccgtgctc cgctggacga cgccggcgga ccgccgcgac
     1321 gatcagtcgc ccggcgctat cgccgcgtac gatctccgct tctctttcga cagcatcacc
     1381 gacgagaact tcgcggccgc ggttccggtc gagccggtgc cggcgccgct cccggccgga
     1441 cacgttcagc agtggactgt cttcggcctg acccccgaca gcggccatta tttcgccctg
     1501 aagtcgcgcg accgccgcgg caactggagc gccgtgtcca actgcccgtg ggtgcactgc
     1561 gccgctgtcc gcccggcggc cttcgccgat ggcgcgctcg agcaggcggt ccgctcccac
     1621 atccacaaac cttccggcga tatcctgacg tccgacatcg ataccatcgg ccagctcgtc
     1681 gtccgggacg ccggcatcgc ctcgctcgcg ggtctggagg actgccggtc tctggcggcc
     1741 gtggacttcg cccgcaatga tatcaccagc ctggcgccgc tctccggcct tcctctgctg
     1801 agcggcctct atctcgccga aaacaacgtc agcgatctca accccctggc gggtatggca
     1861 agcctccgcc agattcagct tcaggataac cccgtcagcg acctgggccc gctggcgacc
     1921 attgactccc tccagcagct catcctctgg gggaccccgg tgactgattt ctccgctctc
     1981 tacgaactcc gttttctcag cgacgtcggc ttcgaccgca tgaaccttgc cgacatcagc
     2041 ttcatgagcc acctgaagcg gccgcagatc tgcgggctcg ctttcaacaa catcacctcc
     2101 ctcgaaccgc tccgcccgct gtacatgctc gagtcgctga acctcatgca gaatcacatc
     2161 accgatctcg ccgggttgac gcccctgacc ggcctgcggg aactcaatct cgccaataac
     2221 cagatcgttg atctgttgcc cctgatctac aacaccggcc ttgattcggg cgacgtcatc
     2281 cggctggagg gaaacccgct ctccgactat gcgcgagcga tccagatacc gaccctccgg
     2341 ttgcggaaag tgactgtcat tttctgaatc gtgcggtcgg cctttcggag cggatcgcgg
     2401 ccggcaaaca ctccaaaaaa ataccggccg ggctgcagcc cggccggcat cattgtcata
     2461 ccgaccgccc ttgcggccaa ggtcacgccc gcggcatgcc gagcttcacc tgcgcatcgt
     2521 gcagcttgat ccgccgcgcg acaatgtctt tctggcggga agccagcttc tgcgtcacgc
     2581 ccgcctgttc ctgctgcagc ttcagcagct tcacttcaat ttctccgagc acctgttgcg
     2641 ccgccgtctg cgaaagcggg ctggcgcctg ccgtgctcag cgaatcgtac tcgatccgtt
     2701 tccgctggag ctcggcctcc agttggagga cgccgatacg gcgctccgcc aactccccct
     2761 ccacctgcgc ctgctcgatc tccgccttcc gcaaatcctt cagccgcttg agcaggtcga
     2821 tcgcctgctg ttcgactttt ttctcgatat cgagggcaat cacctcggac tgccgctgcc
     2881 ccttcttggc ctctcccttg cgccggtcga cgtccttgag cgccgtctgt ttctgctcga
     2941 tcgtgagctc gatctctttc agacgccctt cggcctgcgc cttctgggcg gccgccattt
     3001 ccttcaccat cgtggcgttg tcgatgtcgg ccgtcacttc cgccacactg cgcatgggaa
     3061 tgacgaccat gcgcgaatcc actgcgcccg accgagccgg ctcctgcgcg gagccggtgg
     3121 ccgccgccag gatcaccaca cccagtgcga gaagtccctt cttcagcttc atgtaaacaa
     3181 cctccggccg gcctcgaccg gcccgtttga gaacatacct ccggggccgc cgtaacataa
     3241 ggcccgcgcc gccggtgtca acggccttta taccgaattc ctgtcttcgc ggacggcctg
     3301 gtccgacggc ggctgtcagc gggggatttt tctttgtcga tccccggcag cattgtatac
     3361 tgccgtgcga ggcgaaggag gtacgcatgg atgctctttt gattgcgaaa gccggccgcg
     3421 atctgcacct gttgccgcgc atggcgaatc gccacggcat gatcgcgggc gccaccggga
     3481 ccggcaagac cgtcaccctc caagtcatgg ccgagcagtt cagccgcctc ggcgtgcccg
     3541 tttttctcgc cgatgtcaag ggggacctcg ccggtctcag tcagcccggc ggaggcaacg
     3601 cccgggtcga cgaacgcctg aaggcgctcg gcgtcaacga ctggatctgg cagggttgcc
     3661 cggtgcgatt ctgggatgtc tacggcgagc agggccatcc cgtccgcact accgtctccg
     3721 acatgggccc tctgttgctg agccgcctcc tcgacctcaa cgacacccag gccggggtgc
     3781 tcagcctcgt gtttcgcctc gccgacgaca acggcctgct gctcgtggac atgaaggatc
     3841 tccgcgccat gctccagtac gccgccgaca atagccggcg cattcagact gcgtacggca
     3901 acatctcccc cgcctcggtc ggcgccattc agcgcgctct gctcgcgctg gaggagcagg
     3961 gaggcgaccg gcttctgggc gaaccggcgc tcgacatcga tgatctccgg cagaccgacg
     4021 gcgacggccg cggggtggtc aacatcctcg ccgccgaccg gctcctgcag accccccgcg
     4081 tctataccac ctttctcctt tggctgctct ccgagttgtt cgagcagctc cccgaggccg
     4141 gcgacctgga caagccgaaa ctggtcttct tcttcgacga ggcgcacttg ctcttcgacg
     4201 aagcgcccaa agtgctggtg gagaaaatcg agcaggtcgt gcggctcatc cgctcccggg
     4261 gggtgggcgt ctacttcatc agccagaacc cgctggatgt gccggacgac gtcctcggac
     4321 agctcggcaa ccgggtccag cacgcgctgc gcgccttcac ccccaaagac cagaaagcgg
     4381 tgcgctcggc cgccgagact ttccgcgcca acccggccct ggatgtagag cgagcgatca
     4441 ccgaactggc cacgggcgag gcgctcgtgt cgctgctgga cgcccagggc agcccatcgc
     4501 cggtcgagcg ggcgctgatc tatccgcccc acagccgcat cggcgccatc gcgccggaga
     4561 cccgccgcga cctcatcggg caatcgccgc tgcggggccg ctacgagacc ctcctcgacc
     4621 gcgagtctgc ttacgaaatg ctcagagcgc gcgccgatcg ggcggccgcg gacggcgcct
     4681 ctccctcccc gcggccgggc cggccgtcgt ccggggcgtc gggcaacgcc gccaaaatcg
     4741 ccacttccat cgcgcgctcg gccggcagcc agctcggtcg cgaattggtg cgcggggtgc
     4801 tcgggtcgct cttcggccgc cgccgacgct agtagagcgc gggtggactc cgccctccgt
     4861 agccggggcg cgccctcctc gccgcccgct cagccggaga accgcacttc gcggtacgcg
     4921 tccaatcttt taacttgccc catcatcgac aggtgggttc attattcagt aggggcgact
     4981 ttcatttacc gcgtctgtgc cggagtggag cacagatgca ctcactgccc gagccccaac
     5041 tgtggtcgaa gtgaaaggag caacgacatg ggaaacctct tatcaatcat tatccagctc
     5101 atctgcggcg ccgtcggcgg caacgtcgcc gggtcgctga tgaaaaaatc ttcgttgggc
     5161 acgatcggaa attccatcgc cggcattctg ggcggcggca tcggcggcca gattctcggc
     5221 gcgctcggcg tcggcgcggc agggggcggg acggatgtcg ggagcattgt cggcagcatc
     5281 ctcggcggcg gtgtcggcgg cggcctgctc atggccatcg tcggtgtgat caagaaagcg
     5341 atgtcgggcc agaaatccta aacacgcgcc cgccggccgg gccctgacgc tcggagactt
     5401 cctgcaccac ggcagccccg ctaacggctg ccgttgtgcg tattgaagac tggcagccgg
     5461 gggcgcctct cctccgctct gcgctcgatc gcattcctgc aagctgcccc tcgtgaccca
     5521 tgcattccac cccgcacccg gtgtacgcct gaccctgcct gcgtgcgccg ccgttgccgt
     5581 tggcacgatc tcttcaactc tccgtcttca gctaaagaaa cgccgcgctc cgactgcccc
     5641 ctccccctta accctatgtt gcgccttaag atagactgtt tgcgccttcg gctgacttga
     5701 ctgcttgccg ttcagccagg attcaataat actatcacca gcggcaaaaa cctgcgaacg
     5761 aaggtgataa ccccgatgag cgagctgatc gacaacgcca aggcccgtca ggcggtcctc
     5821 aaacacctga tcctccaact ccacgaggga aaagccccgc aggaggtcaa acctcaattg
     5881 ctccggctcc tcggccgggt cccttaccac gaagtcgtcg ccgtcgaaca ggaactcatc
     5941 aaggacggcg tcccggtgga ggacatcctg cggctgtgcg acctgcactc ggccgccatg
     6001 aagggagcgc tcgattcatc gggaccgccc gagcttccgc ccggccatcc ggtcgacact
     6061 ttccggaagg agaaccaggc cctcatcggc gagctggaac tcgcggacag gctcttcgcc
     6121 gaaatcgagg cgctccccga ggaggccgat gcggaggaac ctctcgggca cctccgggtc
     6181 cgtttcagcc gtctggccga tgtcggcaag cactatagcc gcaaggaaaa cctcgtcttc
     6241 ccctatctcg aacagaaagg catcaccgga ccgccgaccg tgatgtgggg gaaggacgac
     6301 gaggtccgcg ccgccgtgaa gggcgctctc gaagcgctcg cctactccgg ccgggtgtcc
     6361 gtcggcgacg cgaaaggggt gatcgatctg gtcctccgtc cggcggtcga ggcgatcagg
     6421 gagatgatct tcaaggaaga ggagatcctc tttcccatgt gcctggagaa actcaccgat
     6481 gccgagtggt acgccatcgc ccgccagagc cccgcgatcg gcttctgcat ctatgatccg
     6541 gtcgaggact ggcagccgga gggtccggcc gcggaaaccg agacggcgtc tccggccgcg
     6601 cccgaccgcc tccagttccc cagcggcagc ctgagcccgg tcgagatcca aaccatcctc
     6661 aacaccatcc ccttcgatct caccttcgtc ggcgccgatg acacggtcag gtatttcacc
     6721 caaggtcggg aacgcatctt cgagcgcaac cgcgcgatca tcggccggaa agtgcagatg
     6781 tgccacccgc cgggcagcgt gcatatcgtg cagaggatca tcgacgactt caaagccggc
     6841 cgcgccgatc gcgcgccctt ctggatcgag ctccacggca agttcattca cattgaatac
     6901 ttcgccgtcc gcgacgagca cggaacctac ctcggcacgc tcgaggtgag ccaggacctg
     6961 accggcaagc gcgccctgac cggccagaaa cggctcctct cctacgagta ggcgggcgcc
     7021 atggccgaga cgccccgacc gctcatcacg ccgcagacca aagtcggcga tttgctcgat
     7081 gcttacccgg agctggaacc gctgctgatc gaaatcgcgc tacctttccg gaagctccgc
     7141 aacccggtcc tgcgccgcac ggtcgcgcgg gtgaccagcc tcgcgcaggc cgcccgcgtc
     7201 ggtaacgtct ctgtcgtcga attggtgcgc cgcctgcgcc gggccgccgg ccaacccgat
     7261 ttcgtcgccg acgtctccac cgctccggcg gcttctcccg gctccgcgcc gccgttctgg
     7321 atggatccgt cggcggtcat cgagacgctc gatgcgcggc cgatgattga cgccggcgag
     7381 cagccgctgg gcgcggtgat gcgggccctc gcccggctcc aagccggaca gatctgcgag
     7441 gtgatcaccc ccttcgaacc ggcgccgctg atcgacaaag ctgccgcccg cggctaccgg
     7501 gtgtggaccg ccgcgcccgc cgccggcgaa ttccgcacct atttcacccc cgaatgaccg
     7561 caccgaggcc gcggccggtc agtgcgccgc ccggctcccg tcggcccggc cgagcgcggc
     7621 cggcgtctcc gcggtttccc tgggccgcgc gccgcgccgc agatggatcg ccagtcccgc
     7681 cagcgagacg atgaggaccg ccccgcccgc ggccgcgatc aggtgcgccg gctgcgtccc
     7741 gcgggcgtac tcgtgaaacg cccgcggcgc cccctgcgct ccgacgacgg ccagcgggag
     7801 gaaagtcagg agcagccccg ctgtcatgcc gtagatccac cgcgccgccg cccgcccggc
     7861 gcaccgcggg tcgccgctcc ggctgcgcgc gtgaatcgtc ccggcgacaa acgccatcac
     7921 cccgatcccc agcgcgccga aatggaggtg ccccacggtc gcatatgtgt cctgcagatg
     7981 cacgccgagt cccggcgccg ccagcgcgct cccgctcgcc gccagcgccg caaaagcggc
     8041 cgcctgtccg aacagcgcgg cgttgagcgc cggttccgcg agcctcgccc tcagccacgc
     8101 ccgcaggagg agcccgaata tcaccgtggc cggcacgatc gccagaaggc cgtagagact
     8161 cccggccgcc gccatccgcg aaccctcggc cgccgtgagc aggtgctgcc cccacgagac
     8221 gagactgagc agcgccaccg ccatgccggc cgtgatcagg gtgcgccggg gcagacgcac
     8281 cggccggccg gcgccgccga gtagatcgag agcaatcccg gccgccggca gcaccaccgc
     8341 gaggatcgcc gggttcgcgt acatccagaa cagcgtctgc tgaaacgaca ccgggtccgc
     8401 cgccccgaac cacgcggcgg ccgattccgc cagtccggcg ctgctttgaa ggagaagaaa
     8461 gaacagccga atcggaacca ccgccgtctg gaccagcgac cccaacagaa agaaccaggc
     8521 caaaagcggc atctccgcca ttggcacggt ccggtgccgc cggctgagaa tcgtccacgc
     8581 cagcgccaca ttcaccagcg tggtcgctat cgccgccgcg gccagcccgg ccgccagcac
     8641 accgtaagct cgctcggtcc ccacttccgg cggcaggatc attctccatc ccccctggta
     8701 cccgctcagt tccgctcccg caatcactcc cgccgccccc agcacatatg caccccaccc
     8761 ggccagagtc aggcggggca gcgccgtctg cgccgctccc acggcctgcg gcacgagcag
     8821 attgccgatc acggcgggca gcatcgggat cagcacgaga aacaccatca ccgtcccgtg
     8881 cagcgtgagg accgctccga accgcgtgtt gtcgagcaga tcgggccgcg gcgtggccag
     8941 ctccgccagc agcgccgccg ccgccgccag cccgatcgca aacgcgatgc cgatgccggc
     9001 ggcgtagagg cgggcaatcc ccgccgcccg cgccgccgcg tagacccggc gcgggcgcag
     9061 gcgatcgatt ctcccgcgaa agacctcacg catgctcaaa aatccttccg ggcgaagcgg
     9121 cggattccat accacacggg cgccgccgcc cagagcgtca gcaccgccgc cgccagcccc
     9181 agcccgctcg cgcggtcgaa aaagcgcatg aacaccgccc ccgtgtaccc catcagcgcc
     9241 gccatatccg aatgaatgag cagcagcacg cgggcgagat cgaccgggtt gcccagcgcg
     9301 gccgccaaaa gaggcgtctc cagcggatag gccgcgaaag cagtcgccgc cgccagcacc
     9361 gctccgtcgt agccgatcgc cgccgtcagc cagattccca ggaccaccgc cagccccttg
     9421 ccgcggtcgt cgaagcgcac ggccgccacc agcgccagcc ccgtgaagat cgcgccgagc
     9481 gccgtgccga ccaacagcac cgccgccagc gctgtcatcc ccgccgcgtt cacacccccc
     9541 aggacgaggt agggcacgcc gatccccagg gcgaacccga gccagagcga ggcgaatatc
     9601 ccggcgaaca gcccgagata caccgcgcgc cggcccaccg gctgggtgag gatcagctcc
     9661 acgaactccc gcgcgctgta gtagtacatc gccgggtaga gcagactgat cagcggcaca
     9721 aacaccagac agagactgct cagacttacg gccgctttcc cggcgtccgc ctggagatag
     9781 aacagccccg ccgtggccgc ggcgaaaaag agcgtgttca caaggctcca gcggctccgg
     9841 acaatatctt tcaccacgaa ccaggtgacg ttccgcagcg ccgtcatgcc gccccccgcg
     9901 ccagcatctg cgccaccgcc gcctccaggt acggactccc cgtggccagc ttcaactccc
     9961 gcaccgatcc ggcaaactgc accgcgccgt cgagcagcag caccacgtcg tccgcaagtt
    10021 cctcgaggtc gccgatgatg tgggtcgtca gcagcaccgc ccggccctcc ccttttcgct
    10081 cgaggacatg ctccttgagc acccggctgg ctcccggatc gagaccggcc gtcggttcat
    10141 ccagaatgag caccgccgga tcatacaggg cggccaccgc cgcgctcagc ttctgccggg
    10201 tcccgcccga gagcgtccgc acccgcttgt cgaggtgggc gtgcaggccg aaccgctcga
    10261 ggagcgcgcc gccggatgcc ggccgctccc cgcggaggcg caccaccagg tcaatcacct
    10321 cgcgccccgt cagatcctgg ggaaaggtcg ccgcctgcgg catgtacccg atccgcctcc
    10381 ggtactgccc ggccgcgtcc ggcagccgtc cgtcgtagag aatcactccc tcgctcggcc
    10441 gcgccagtcc gaccaggcac ttgatcagcg tcgacttccc cgacccgttc ggcccgatca
    10501 cccccgtgat ccggccggga gttacgtcga acgacacgtc gcgcagcgcg acaaagcggc
    10561 ggaatcgttt ggtcaacccg cgcgtttcaa tcataggttc ggcctcattc gcgggcggcg
    10621 gtccacgagc gtttccggcg tcagcgtcgg catcgcccgc tccgccaggt cgaggacttc
    10681 caccagcaga ctgcgcagga gcaccaacgt cggcggatgc gactccacca ggagcgaaaa
    10741 caggctcacc ggacgaaagg gcagatcgcc gtaaccgtcg cgatccaaat ccaccccccg
    10801 gtagccgctc cagtagttcc cctcgaaacg gctgttgttc tgacggctgt tggtggctac
    10861 ggcgaacgaa ttgtcgacaa agtcattcgc gctcaccgta ttgtcgaggc agttcgccat
    10921 cagcttgagc gcccagccgt tgccggcaaa gagattcccc gcgacataca cgcggtccga
    10981 tccctccatg tgtacgccgg tcgagttgtc gcagaaccgg ttccccgtca cccggctgtc
    11041 gcggatatcc tttagcagca cgccgtagga cgccccgccc cagctttgct cgaacgtatt
    11101 gttctccatc aaaacattgc tcgtgtacat caccgccacc ccggcgccgt tccggcgaaa
    11161 caggttgtcg gcgtactgac agctgtcgga gaacatgaaa tgcagcccgt agcggaggtt
    11221 ctcctcgcag cggttgcggt cgaccgtgcc ggcgcgcacg aactccatgt agatcccgtc
    11281 ccgatgcccc gcgatcgtgt tctccagaat cgtgatatcg cggcagtacc agaggtggat
    11341 gccgttcccc gatgaggtca ggcgcgtctg cgctccccgg atgatgttgc cctgcagccg
    11401 gcaggtgtgg gagttcgccg cgtaaatgcc gaaaaagcac gcctcgatcg agttgttctc
    11461 gatcgtcgcc cgtaccgccc cttccagctg gatcgccgcg tgctcggccg tgaaactcgt
    11521 cggcaccccg cgcagccgga gattccggat ctccacgtcc ggcgcggtga ccgtgaatat
    11581 gccccccgcc ccgtccgcat cgaccaccgc gccctccccg ccggccagcg tgagcggacg
    11641 atcgatcgtg atcccgtgct cgcggtagac ccctgctttc accgccaccg tatcgtgcgc
    11701 gcgtgccgcg gccacgcccg cagcgaccgt ggcgtgctcg ccgccgggct gcaccgtgac
    11761 cacccgcgcc gacgccaggc ccgtcacagt cagcgcggcg cccagcgccg ccgcgagctt
    11821 tcggccgaat ctccgcccgc ccgcgggcca cccgcgccgc cgcttacttg atatcccacg
    11881 catccgccac atacgcccgc acctcttccc aggtgactgt ccgtccgcca ctctcggcgg
    11941 cgagccgttg gccggcctcc tccgacgcgg tggccgccag tcccagtccc atcggactgc
    12001 gcagccgctc ccccgccacc acgaccgctt tcgccccggc cagaaaagtc cccggatcgt
    12061 tgacgtcggc cagccagcgc gccgaggcgt cgatgtccgc ctggtgggcc aactcgtagg
    12121 ccgccaggca ttcgatcgaa tcgaaccgga acacctttcc tttcccggag atgagctggg
    12181 tcccgaacgc gggatcggcg atgttcatcc ggcagtagtc gcaccggtcc tcgccgtagt
    12241 gcatggcggc gggacccttt ccgccgcatc cggccagcgc caccgcggcg gctaccgctg
    12301 ccgccggaat cagcctaacc cgcatcgccc gcctccccgg agattccctt tcgacgcgcg
    12361 cgaatcacct ccgcgccgta gaccgcggtg ccgaccgcga acacaccgag cgccacccac
    12421 ccggccggtc ccggccacga gtgcgcctcg aaattgagca gccgcttcga cccgatcagc
    12481 ggcggctggt aggtcatccc cggcaccttg ataatcgcct gctcggtatc gaggttgtgt
    12541 ccgtagtcgt acccccaccg gtagaaatcg atcatccccc ccgccgcgac ggcggcgaac
    12601 agcaccaacc atacgagcac ccaggtccgc cgtccgaccg ccgcggcggc cagcccgagc
    12661 agcatcaggc cgcgcatcac ccagggcata atccgcagct ccttgatcga ctccgggtga
    12721 atggccttca taccgatata atggttgagg gtgttgatct tctcgatgtc ccccgcgttc
    12781 tgcccctcca tccggttgat ccagatctcc agccccagcc cctccgggta ctgcggcgcc
    12841 tccagggcga tttcccacag cgggaggaaa taggtcaggg ccaacagcag ggccgcggcc
    12901 gccagcgcga tccgtccggc cgggtgcatc cggcctcgct tccccgtcgc ctcggaccgc
    12961 caggcggccc ggtcgtccgc ctcgcggaga atcgcgggac tcatcgccgg gcccgccctg
    13021 tggtttcaga tcgggtcatt tcgacacccg gatgtaccct tgcatctcct ggtgcagagc
    13081 cgagcagaaa tccgtgcagt agaacggata cacgcccggc gcctgcggtt tccacgccag
    13141 cgtccgcgtc tcccccggca taatcagcaa ttcggcgttg ttcgccccca tcaccgcgaa
    13201 gccgtgcggg gcgtcccagt cctgctcgag gttggtcaca tggaacagca ccgagtcccc
    13261 cgccttgatc ccctcgatat tgtccggcgc gaaatgcgtc cggattgtcg acatgtacac
    13321 acgcacgagc ttcccgctcc gctcgatgcg cgcctggttc tccgccgtga tcgcgtgcgg
    13381 attccggttc tcggcgatcg ggaagtactt caccgatttg tccttgatca gcgcggccgg
    13441 cagcgcctgc gcgtagtgcg gctccccgat cgtcgggaaa tccagcagga gcttcatctt
    13501 gtcgccggtg atgtcgtaga gctgggctga ctgcgagagc tccggaccgg tcggtaggta
    13561 ccggtccttg gtgatcttgt tcagcgccac cacgtacttg ccgaaaggcg cccggctgtc
    13621 cccgcccggt atcatcaggt gcccgattga gtagtacgcc ggcgcccggt caaccacctc
    13681 ccacgtcccg agcttccact tcacgatctc cgaggaaatg aacatcgagg tgtaggcgta
    13741 cccctggccg tcgaactccg tatggagcgg gccgagaccc gggttctgca cctcccccgc
    13801 gagcaccgcc tcgtacttga gcaccgggat gccgtcgacc tccccctcga actcccggtt
    13861 ctcgatcgct ttgatcatcc gggagaacga gtgcaccggg atcaccgtcg ccagcttccc
    13921 cccgccgaca atgtactccc cggtcgggtc gacgtccgcc ccgtgcggcg acttcggcgt
    13981 cggaatgtag tagatcatcc ccggacaatc ccgggggtcc aggattttga ccgagccgac
    14041 cacctcgctc tgcgccacgc gggcctcgtc gagaaaattg tgccggtacg aggcgggcac
    14101 gtctttagcc ttcccctccg cggcgtaccg ctcggccagc ttccagttga cggccgccat
    14161 gtagtccttg tcgttgcggg aggcattcac ctccagcagc gtgttcgcct gctccgtgtt
    14221 gtacgtggag aagaacgccc agccgtgcga tggtcctttt ccggcgtgcg agaggtcgta
    14281 gtcgaacccg ggcacgagaa tctggaacgc cagcgacatc cgccccgtcg ccgagtccac
    14341 cttgatgaac gacagcgtcc ccttgaaatt ctccttgtag gtgctcaccg gcacgtcctg
    14401 ctgcgggatt ggcacgctga accgcgtccc cgccatgaca tattcggtgt tgggcgtgag
    14461 aaagggggag gcatggttgc cgccggaatg cggcaattcg atgatctccg tcgtctcgaa
    14521 cgtggtcaga tccagccgcg ccacccgcgg cgtgttgttc ccgttgatga acagccaccg
    14581 cccgtctgga accccgtccg tctgggagag ttgcggatgg tgcgcgtcat cccacggcac
    14641 aaacccgaac gacgtcatca gcatgttctt cgtctcttcg ctgtagccgt agccattctc
    14701 ggcgtactgc gaaaacaccg gcacgtgccg cagcagacgc cccgagggca gtccgtagac
    14761 gctcacttgc cccgaaaatc cccccgacat gaacgcgtag aactcatcgt acgtcccggg
    14821 cgccacataa acccgcgccg cggcatcgcc ggtaccggcg acaccttcgt tgccgccggt
    14881 accgcatccg ccaagtagga ctaacgcaac cagcccggcc aggccggaaa ggattttccc
    14941 agataacctc cgaaccatac ttctctctcc tctcaccaca tcacgaaacg tgctcggttt
    15001 cttcgctcca ctcagttggc cggcggcggc ggcgggacat tcgcccccgg ctgctgctca
    15061 agcggcaccg gcggcttgtc ggcctccgac cgaaggaaat ccaggagcgc ccgcgcgtca
    15121 tccggcgtaa tcccctggat cgtcatgaat tgtgcgtact gcgccaccag cttcttccct
    15181 tccggatgaa gcttccccat ctgctccggg ttgagtactt ggttcagcac atactccgga
    15241 ctacggcgct tggttacatc gcgcagtccc ggaccggttt tccggtcatc cagatagtgg
    15301 caagtcgcgc acttggcgat aaagagctcc cgcccctgcg ccgctcgcgc tgcatcgacc
    15361 ggaccgatct cgagtgcgtc cgtgatcggg ccgatcccgt gttcgagctc ccagccggtc
    15421 agcttcccct gctgccagcg ggtctggcgc tccgcctccg tttccggccc cgaggaacat
    15481 cccatcaacc acagagttag agcaaacccc gcgaccccga tcaacttcct gaactctacc
    15541 gtcatgacaa actacctctt ctgtcggtta ccggctggac cccgattcac acccggagtc
    15601 tgaccgccta taacaagatt tcggagtatg aaatccgtaa tattgccgtg ctcagaccgc
    15661 tcgcgcggcc tgcgcgatta tctccttttt ccctccgccg atcctttcgg cacaaggtcg
    15721 gccagcgtcg tccgggtcag catggcccgc agatcctccc gccgcttccg ccaggcctcg
    15781 tgcagcgggc agggcgtctt gtcgctgcac tgcgggagtc ccagtgcgca cccctcgaac
    15841 aaccccatcc cgtcgattgc agcgatcacc tcgatcaccc gaatttgcct ggccggccgc
    15901 accaagccca cccctccgtt cggtccgcga aaggactcca tgatctcgac ctccgtcaac
    15961 ttgcggagga tcttggtgag aaagtggaac gagacgccga cctcatcggc cagctcgcgg
    16021 atcgggataa acgcccgccg cccgtcggga tctttggccg cggcgagcac ggcggcgcgg
    16081 atcgcgtagc tgcacgcttt ggaaaacagc atcttttcgg ttcacattcc agttttagga
    16141 ctcttttatc tgaaatatta tatcgccgtt catttctgtc aagcccattt tgctgtttca
    16201 cggcacaaaa ttcgcccggt ggacgcctct gtcgtttgcg tccctgcccg gcatccttgt
    16261 gcgcaggatg ttctcggcag ccaacaactc ttacatcaat gacttatggt gtacttgcag
    16321 gagtagccga tagtcccctc aaatctgcct gcctctttcc cagcctcgcc gcgcgcttca
    16381 ttatatgtcg tcagcttccg ccacccgccg agtcaaaccc ctccttcggt cgttccaggc
    16441 cgggcgccgc tacggtaccg cacgtcaatc gggcggcgag ccggccacca ccgacccgtc
    16501 gttcatatcg ataatccacg ccgtccaccg ccggtaaccc gctgatgctt tgacctggta
    16561 gagatgtccg gcctgcgcct caaacgacag cgtcatgggc tcatcggagg tccccttcca
    16621 cgaggagtag ttcaggacca gcgtgtgttc tcccggaagt acctcgatgt gctcttccga
    16681 gagcgctcgg tagcggtgga ccagcgtgtc gatccccatc acgtacagcg acttgtgact
    16741 gacatcgaga accgcgacct gctccgccgg ccgacgctcg ccctcgtaga gcaccagcgt
    16801 ccggctccct ccgcacgaga ggagagcgag tgcggtcagc gccaaagtta aaagcagtcc
    16861 cgttcgcttg ttcatggccg atttcctcat gcctcatggt cgtcgctgtt cgggtgggaa
    16921 gcaagcggaa gttttgccag acacggttga ttctgaccgt gcacaatgtg acactcaccg
    16981 gccgattggt gatttacttt ctccgccgat tgaccgtatt cattggggcg ggacacaatc
    17041 gggaggaaag cgcgagggcc ggcgcccccc tttcgtggcg accgactcgg gggataaccg
    17101 ggatctcgtg aagattgatc aaacgaacga accgctccgt cctaccgggc gggtcacaag
    17161 aggcgtgtcg cttcttgccg atggcggcga gacagcgccg gtgccgccgc tcacaccgtg
    17221 cggccgtcct caggcaggcg tacttccggg caccgcctgt ccctcgggct cgcggtcgct
    17281 ggtggcgacc agttcggcca gttgcgcggc cggcgccgcc gccaactgct gcgccgtcaa
    17341 ccgccaggtc cagttgccgt ttcgggtcga gggcgtgttc atgcgcgcgg accccggcaa
    17401 gccgagcaca tcttgcatcg ggatcaccgc cagccgcgcc cgcgactgca gggccagttc
    17461 gatcatggcc cagtgcaccc ggtctctcgg cacggcccgg ctcaagtgcg cgaacaaccg
    17521 tttcttctcg gccggcttcg cctcctcctc gaaccacccc tgcaccgtgt tgttgtcgtg
    17581 cgtcccggta tacaccaccg cgttctcatc gtagttgcgc ggcaggtacg gatgggttgg
    17641 atcatccgct ccgaaagcga acaggagcac cttcatgccg gggaatccgt accgcttcat
    17701 cgtggctgtc acatcgtcgg tgatcagtcc gagatcctcc gccaccacgg gcaggcgccc
    17761 gaagtgccgt tgcagcgtct gcaggaaatc atcggtcggt acttcgcgcc atttcccctt
    17821 ctgcgcggtc ttgtgtcccg ccggcacctc ccagtaggcg acgaggccac ggaaatggtc
    17881 gatgcgcacg atgtcgtaga gacggaaaac gtgagccagc cggtcgatcc accaccggta
    17941 cccttccctt ttgagcgcct cccagtcgta caccgggttt ccccagagct gtccgatcgt
    18001 gctgaagtag tccggcggga cgcccgccac cacttccggc cgcttgccgc cgttcagttt
    18061 gaacaggtgg gtgtcgcgcc aggtgtcggc gctgtcgtag ttgacgtaaa tggggatgtc
    18121 gccgaacatc cgtatcccgc gggcattggc ctcccgcttg agggcgagcc actggcggaa
    18181 gaacagccac tgggcgaact gctcgcgccc gagctcatcg gcatgaacct tccgtacctc
    18241 gtccagcgcg cgggggttgc ggtcgcgcag cccgaccggc caccggctcc agtccgcccc
    18301 ctgctgggcc cgtttgatca cgacgaagag cgcgtagtct tccagccagc ccgcttcttc
    18361 ccggcagaac tcccggaact cctcctggtg cgggcggtcc cggaaggcct gccaggcccg
    18421 gtccaaaagc cgcgtcttgt acgcggtcgc ttgttcataa tccgcgcggt catccggcgc
    18481 cgccgggatc ggctgcagct cctccgcccc cagaaaaccg tcctccacca tccggtccgg
    18541 gctgatcagg agagggttgc ccgcgaagga tgaactgctg ctgtaggggg aattggcatg
    18601 cgccggatcg gttggattca gcggcagcac ctgccataca ccctgccggg cgcgggcgag
    18661 aaaatcgagg aaccgggaag ctcccggccc gaaatcgccg atcccgtaca ctgagggcag
    18721 cgccgtgatg ggcagcagga taccgctcgt ccgtctatac tcggtcatca tctctcctcg
    18781 cgcggcggcg aaccgccgct gtcccgcacc tcgtagacgc ggcccggaaa gcgccgtggc
    18841 gcgctcgata gtccgccttg cgcatcattg actccgacca gcctcggcgc cgcaccatgc
    18901 gaacgcaatt atagtatcgg cgcgccgggc gcgaatccca aggggagaaa agacccatac
    18961 ccgcgcagcc gaccgcaccc tgtcggtcgg aggtcgcacc ccgttgtaca gccgaacccg
    19021 gccgttcgcc atcaacggaa ccggcttacc ccgcgcgacc gcccacactt cggggcgagc
    19081 gacaaccggt tgtcctgtca agctctgtct gggtaattgc gctgccggcc ggccgaatcc
    19141 acagattcgg ccggccggga cacatgcttg tcgcgagtgc agagaggaga tgggtggcgg
    19201 aacgtgctgc agagaagcgg ccggtgtcgc tgctcaggcc cggcgctcgg tgggagcgag
    19261 cgtcacgtac gtcgtcttcc cttccacctg gaactcgatc ttgttttcgc gggccagcca
    19321 gcccaccgcc tgaaaggcga gcgactcttt ttccttgacc gccctcggga cctgcgacag
    19381 ggccagctta tccttcttct gaagcacctg ccaaatcgcc cccgcggtcg ctccgatctg
    19441 ctccttcata ctccgtaacc ctaacctttc ctattctgag gacgcgctat cattttgaaa
    19501 gtacgctgct cgcgaatcga ggagcaacta atattcgtac cggctgcgcc gaaaactcgc
    19561 taatccaagt cctgtttctc caatacaacc accgcaaggg gcggaaggtt cagggcgagc
    19621 gactggggga aaccgtgcat gggtaccggc cgggtgtcgc atccgccggc atttccctgg
    19681 ccgccgccgc cgtactcctg tgcgtcgctg ttgagaacct cttgccagta gccgggatag
    19741 ggcgctccga tgagatagcc ggagcgcacc accggcgtga aattgcacac taccagaata
    19801 ctcgcccgcc cctggcggtc tctccgcagg aaactgatga cgctgttggc gtagtcgctg
    19861 ccgtccaccc actggaaacc ctcccagttg aagtcaactt cgtagagggc gggcgtcccc
    19921 cggtagaacc ggttcaggtc ttccacccac cgctggattc cgcggtgctt gggaaactcg
    19981 gtgagatgcc agctgaggct ctcctcgtgg tgccactcgt cccactgccc gaactccccc
    20041 cccatgaaga ggagtttctt gccggggtgc atccacatgt agccgagcat gaggcggagg
    20101 ttggcctgct tctgccaggt gtccccgctc atcttgccca gcagcgaccc cttgccgtga
    20161 accacctcgt cgtgcgacag cgggagttgg aagttttcgt ggaaagcgta gagcaggctg
    20221 aatgtcagcg tgtcgtgatg gtacttccgg tggatgggat ctttggacat gtacaggagg
    20281 gtatcgtgca tccagcccat gttccatttg aggccgaagc ccaggccgcc gagccaagtc
    20341 ggccgcgtga ccatcggcca ggccgtcgac tcctcggcca ccatctgcac gtccggatac
    20401 cgctcgtaca ccacctcgtt catccgtttg agaaaactga tggcctcgag attttcccgg
    20461 ccgccgtact gattggggat ccactcccct tcgttccggg agtagtcgag gtagagcatg
    20521 gaggccaccg catcgacccg cagaccgtcg acgtggtact tatcgagcca gaagagcgag
    20581 ctggagatga ggaaattgcg cacctcgttg cggccgtagt tgaagatgta gctcttccag
    20641 tccgggtgaa atcccttccg cgggtcctcg tgctcgtaca ggtgcgtgcc gtcgaaatac
    20701 accaacccgt gttcgtcgga ggggaagtgc gaggggacga tgtcgaggat gacgccgatg
    20761 tcgttctggt ggaggtagtc cacgaggtac atgaagtcct gcggcgagcc gtagcggctg
    20821 gtcggcgcga aatagccgac tgtctggtaa ccccacgaac cgtaaaacgg gtgctccatc
    20881 acgggcagga gctcgacgtg ggtgaagccc atccgcgcga cgtactccgc cagaagcggc
    20941 gccagctcgc gataggtgag gaaccggttt ccctcttcgg ggacgcgccg ccacgagccg
    21001 agatgcacct cgtacaccgc gatcggcgcc tcgagggcgt tccggcgccg ccgcccgtgc
    21061 atccaggcgc ggtcgttcca ggtgtacgcc aggttccgca ccaccgaggc cgtgcggggg
    21121 gcaagctcgc attcgaacgc gaacgggtcg cccttctcga ccgtgtagcc gttgtgccgc
    21181 gaatgaatga aatacttgta gatggcgccg tcgccgagcc ggggaatgaa cccctcccag
    21241 atcccggaac cgtcccaccg cacgcccagc ggatgcgcgg tgcggttcca gccgttgaaa
    21301 tccccgatca cgctgatcgc ctcggcgttg ggcgcccaga ccgcgaaata cgtccccttg
    21361 atcccgtcca cctccatcgg gtgcgccccc agcttgtcgt acagctggta gtggcttcct
    21421 tctttaaaaa ggtagacgtc gtgttccgtg agccgggtcg gcccgtgtat gacggaggtc
    21481 tcgcgggcgg tcatcgctgc attcgccgtt gtccgattcc gggagtgttg ttcggtcaca
    21541 aagactgtct cctgtgtcta ttatcgatca cgcgggggaa atgtttatcc cctcgcacgc
    21601 ctttcggccg ccggccgggc gccggtcgac ttcataaccg cacttggccg cgcctttgtc
    21661 aagaagaaag gcccccccgt cggccaaggg cggcgcggcc gggagcgcgc atcccgcgga
    21721 gccgtctcag gtcccggcca ggaagccatc gacatcggaa cgcagcgagt aggccagaag
    21781 ttcatgtccc ggcgggaatt gcagcacgcc gatctcccgc gccaggcagt agaccggcag
    21841 ccgcgtctgc tggcagattt ccatggcccg cggcaccacc tcacacccga tcccgtcggc
    21901 gagcgtgcag tggggaaccc agtgcccggg gcgatagtag gtgcggatac cctccccgaa
    21961 ttcggcgaac gagcgtgtga aatcgtcgtg cacccggagc agttgcggcg agggcgtcac
    22021 gccaaaaaac accgccccct tcgtgtccag cggaaacacg ccgaggctcg acaggatgat
    22081 cgggaacacc atgtgcgtgc ctgcgaatcc ggccaggaag cggcggaagg gttcgacccg
    22141 aaggtcctcg cagatgccga gcgtgatgtg gggacggaag ttggccttga tcatgctctc
    22201 ggagacctcc atgtcggcca gccgccgcca gacgtcggtg acgatcgcgg aggcttcttt
    22261 gtccagaaac agacttaccg caaagggcat ggcacagctc ccctcacagg ttcttgccga
    22321 aaaactcttt caggatgaat cgggcggccg gcacccgccg ccgggcatac tcggctctga
    22381 ggacgctatc gtcgtacggc gccgccttgt aggtgacccc taatcccccc gggcgaaact
    22441 ccagcacgca gtagcgcgca agcggacggc agaaacagcc gagagcgccg gggttgatgt
    22501 accgggcccg gccctggaca tccgagaagg gatggctgtg gccgtagaac agcagatcgg
    22561 tgtcaaaagc gccgaagatg cggtcgaggt cggccggggt cggatcggcg attgcggccg
    22621 ggacgaaacc gtccaaggtc gccgacaaac cgtagtggac gaaggtcacc cgcaggccgt
    22681 tgaactcgtg cgggattacg tacggccagt gacgcaggcg ctcgcggaag tcctcgccca
    22741 gctggcgggc ggtccaggcg cagtgttccg cctcgttgcc gtcgatcacg cccgcgagcg
    22801 ggtcgggaat gccgtggaca aacatcgcat cgtggttgcc catgacgcat tccacccgcg
    22861 ggatgctcag cagttcggcc agactctcgg ccgggtacgg gccgatatcc accgcgtcgc
    22921 cggtgtgcac ggccagatcg tagccctctc tctcgatcgc ccggcggaag gcccgcagcg
    22981 ccggcaggtt ggcgtgggta tcagtgaaca cgatgatctt catggccgct ccaccgacat
    23041 tctacggcag cccggcccgc cggacaagca attcatcggc cctccctctt gtcccccgcc
    23101 cgttttcctc gtaccttgca gcaacacccg gcacagtgcg ccgtcctgtg gagaaatgag
    23161 ccgcctatgg aagccttcaa gtaccacgtc tacatctgca atcaggagaa gcccgagggc
    23221 gtgccgtgct gccgcgcccg cgggggcgaa aaactgatcg acgctcttcg ccgcgaggtg
    23281 gcggcgcaag ggctgggcga cgatgtgcac attacgacct gcggctcgct cggcctgtgc
    23341 gcctggggcc ccaacatgat cgtctatccc gagggggtct ggtacaccgg cgtaggggcc
    23401 gaggacgttc ccgagattgt ccgcgagcac ttcgggcgcg gccgtccggt cgagcgactg
    23461 gcgcggcgcg acgccgccgc cgtgcgcgcc gaaatcgaaa ccaaccgggc gaaaatgctc
    23521 gccgggctta aggcccgcga cgcggccggc gtcctcccgg acgagctgat ggccgatttc
    23581 cggggctttc agaccagccg tatgctcctg accgccgtcg agctcgacct cttcacggcc
    23641 gtgggcggct ccgcctcggc cggcgagatc gccgcccggc tcgaggtcga tgagcggggg
    23701 gcggagcagt tgctcgacgc tctcaccgcc tacggcctcc tggagaagca cgggcgccgc
    23761 tacgccaact ccgccgccgc gcgccgctac ctggcggcgg gcgggcccga tgattcccgg
    23821 ttgagcctcc tgcacctcgt cgggctgtgg aaccggtggc acacgctgac cgagtgcatc
    23881 cgcaagggaa cgtcggtgac ctacacggag gcgaaagatc gcgaccccgg ctggaccgag
    23941 gctttcatcg ccgccatgca caagaatgcg gcgctgcgcg ccgaccaggt ggtgggcgcg
    24001 ctgggggtgg cggggttcga gcggatgctc gatgtcggcg gcggctcggg ggcctacgcg
    24061 atttcattcg ccaaggccaa tccgcgcctg cgcgccgagg tgttcgacct cgaaccggtc
    24121 accgcaatcg cccggcggca catcgaccgg gccggcctcg ccgagcgcgt caccacgcgc
    24181 gtcggcgacc tgcacaccac cgagttcggc tcccccttcg atctcgtgtt catctcggcc
    24241 atctgccaca tgaacagccc ggaggagaac cggcagttgt gccacaaggc tttcgccgcc
    24301 ctccgccccg gcggcaccgt cgccatccag gatttcattc tcaacgccga caagaccggc
    24361 ccgttcacgg cggcgctctt ttcgctcaac atgctcgtcg gcacacccgc gggagcttcc
    24421 tacagcatca gcgagtatac cgagtggctg accggcgccg gttttgccga cctgcgcgtc
    24481 gtggccctgc cgggtccgac cgggctcgtg gtcgcctgcc gtccggaggt cggtcctcat
    24541 ggatgacgcc gaattccgcc gccgcgccca cgaactggtc gactggatgg ccgactacct
    24601 cgccaacgtg cgcgcctatc cggtcaagtc ccccgccgcc ccgggcgaca tccggcggca
    24661 gctgcccgac cttcctcccg agaccccgga ggcgttcgac gatatcatgg ccgatttccg
    24721 acggatcatc atgccgggca tcacgcactg gcagcacccc cggttcatgg ggtattttcc
    24781 cgccaacagc agcccggcct cggtgctggc cgagatgctc accgccactc tcggcgtgca
    24841 ggggatgctc tggcagactt cgccggccgc cgccgaactc gaggaacaga tgatgctatg
    24901 gctgggcgcc atgatcggca tcccggccgg ctgggatggc gtcatccagg ataccgcctc
    24961 caccgccacc ctcacctcga tcctcaccgc ccgcgaacag gcgtccgact acggtgtcaa
    25021 ccagaccggc ctccaggacc ggccgcgcta tgccgtgtac ggctcgtcgg aaacgcactc
    25081 gtcgatcgag aaggcggtca agatcgcggg gctcggcagg gagaacctcc gcaaagtggc
    25141 ggtcgatgcg gggtttgcgc tgcggccgga ggtcctcgaa gaggcggtct gccgcgatga
    25201 ggacagcgga attcgcccgc tctgcgcggt ggccacgctc ggcacgaccg gctccacggc
    25261 ggtcgatccc ctccgccgga tcggtgagat ctgccgccgc caccgcctct ggctccatgt
    25321 cgacgccgcc tatgccggga cggccctcgt gctcccggag atgcggtgga tgatcgacgg
    25381 gattgaggag gccgacacgt tcgtgttcaa cccgcacaag tggatgttca ccaacttcga
    25441 ctgctccgcg tacttcgtga aagaccgggg cgctctcatc cggacgttcg agatcctccc
    25501 ggagtatctg aagacgcgcg agacggcggc ggtcaacaac taccgcgact gggggatccc
    25561 gctgggccgg cgcttccgcg cgctgaagtt gtggttcgtc atccgcacct atggcctgtc
    25621 cgggatacgg gaaaaactcc gcctccagat cgggctcgcc cgcggtgtgg cggaccgcat
    25681 catggaatcg gacgatttcg aactgctcgc cccggcgccg ctcaacgtcc tctgtttccg
    25741 ctaccggccg accggggcgg ccgacagcga ggcgctcaac cgactgaacg agcgcctgct
    25801 ggaggaggtc aaccgcacgg gccgggtgtt catcacccac acgaaactca acggggcctt
    25861 cacgctccgc atggtgatcg gccagacgca ggtgaccgcc gatgatgtcg acctcgcctg
    25921 ggagacgatc gccggcacgg cgcgggccct cggccgcggt taggtgagac gcctgacccg
    25981 gcgaaggaga tgagcatggc tgaccagtcc ttccccgaga acgcccccga ggccccggct
    26041 gtgtcggcca ttccgctgcc gctggcgccg gtggccgatg cggaacgtgt gccctcgctg
    26101 gatgtgctcc gcgggttcgc cgtactcggc attctcgcga tcaacatcca gcagatgggc
    26161 ctcccgcagg cggcgctcct gaacccgcac cttcagggag aggccggcct gctgcacact
    26221 atcgcctggg gaggggtgat agttcttttc ctgcagaagt tcatgacgat tttcagcctg
    26281 ctcttcggcg cgggcctggt ggtcatgacc cagcgggccc aggccgccgg aggcgacgcc
    26341 gcctccctcc actaccgccg gatgggctgg ttgctggtga tcggcctgct ccacgcctac
    26401 ctgctctggt ggggagacat cctcttctcc tacgccctgt gcggcatgct cgtctacctc
    26461 ctgcggcggt tgtcgccgcg ccggctgctg attatcggcg ctctcgtctt cctggcgcca
    26521 gcgccgtttg cggccggctt ctccgggctc atcggccacc tgcagtcgaa aggtgaggcg
    26581 gcgtcgctga aacttgaagc cggggagacg ctttccagcg atgaacgagc gtctctggac
    26641 gcctggacga acgttgctgt ggcgctgagc ccggctccag accagatttc aaaggaagtc
    26701 gatgcccacc tgggcagcta cggcggcctg ttcgcgcagc gcgctctgac ggcgctgctg
    26761 tttcaaacgc tcatcctggt gctcttcgcc gggtggcgca tctgcgggct catgctgatc
    26821 ggcatggcgc tcatgaagtg gcgcgtcttc gccgccgaac ggtccaaggc gttctacctg
    26881 cgcctggcgc tcttcggact ggggatcggg ctgccgctcg atctgttcgg cgcatcgctc
    26941 ttggtcagcc gcgacttcgg cgctgccgtc cagatgcttg tctcggggac ggtcaactac
    27001 acggcgagcg cgggcagcgc gctcggctac atcggcatcg tcatgctcct ctgccggtcc
    27061 ggggccgcgc ggcggctcag aaccgggctc gaggcggtcg gacggacggc cctgtcgaac
    27121 tacctgctgc acaccgtggt cttcacgacc gtgttctacg gctacggcct ggggttgttc
    27181 gggaaactcg gtctcatccc gctcttggct ctcatgactc tcatgtggct ggtccagttg
    27241 tccgtcagtc cgcggtggct gcggcatttc cggttcggcc cgatcgagtg gctgtggcgg
    27301 tcccttgcct accggaagag gcagccgttc cggcgcgtgc cgattgtgac ggcgcgggcc
    27361 gccgggtgac gggcgacggc cggtcgtcgg aggggctttc cgtcccgccg cacggtcagc
    27421 cgcacaggac cgccccgccg ttcacgttga gaatctcgcc ggtgatgaaa gtcgcgaggt
    27481 cggacgccat gaaaaggatc ggcccggcca cctcctcggg ttgcgccacc cgccgcatcg
    27541 gaatggccgc caacaccttc tccccctccg ctccgcccag cgaggggatc gacatatcgg
    27601 tgtacaccca ccccggagcc acgcagttga cccggatgtt gtactcgacc agctcgggcg
    27661 cgagcgactt ggtcaggctg ataaccgccc ccttggtcgc cgcataaggg gagtggaacg
    27721 cctcgccccg ctgcccggcg gtggaggcga tgttgatgat gacgccggag cgctgccgct
    27781 tcatgtgcgg cgtgaccgcc atgcacacgt ggaacgtccc gaggaggttg atgtcgaccg
    27841 tttcgcgcag ttgcgcctcg gtcatttcgt cgatcgggtg atggagccac acgccggcat
    27901 tgttgaccgc cgcgtcgatc cgcccgaacc ggtcgatggt ctccgccacc atgcggtcga
    27961 ccgccgcttt gtcggccacg tcggcctgga ttgcggccgc cttgacgccg tgccgcgcgc
    28021 aggccgcctg cacctccaga gccgccgccc ggtcgcggcg gtagttgatg atgacatggc
    28081 tgccggcccg ggcaaacaac tcggccgccg cccggccgat cccccgcgac ccccccgtta
    28141 tgagcgtcac gcggtccttc atttcaatca tctgggtctc catcggttgg acggttcttt
    28201 ccttttgctc ccggaaacta ccgggggagg ggggttctgc caagaggatt ccgcgggcgg
    28261 gccggagacg ggcgccggct ccgaggaacc tcggcccgaa aaaaaggtgg acaaaaacgt
    28321 gccatttctg gtatattggg gtcaaccgta tcggatttcc gcgccggtct tcgacccggc
    28381 gcatcgccat cgaaagagga aaagtggatt ctctgtcgat tgagtgcgaa aggaggttct
    28441 gggcgtcctc ctgtccgggc tgaccccccg ccgatcggaa agcatcgcac gaaactctta
    28501 cgaggagcaa gattcatgaa gaggctgtta ttactgctcg gcctgtgcgg ttccctgatg
    28561 ctgctcttcg ggtgcagtca ggatacttcg gtcaccggac cgtcgggtga tcaaagtcag
    28621 ctcggacaac tggtgcagcg cccggagttc gtcgaccacc gcactgatgc acaaatcgcc
    28681 ctcacgctca agaacgcccc caccaactgg agcgcctaca agccgccgaa accgccgccc
    28741 gacacaggcg gggacccgaa cccgaacccg gcccacaagt atgcctatgt cgtcggcatc
    28801 tccgactatg aggggacggc gaacgacctg aactactgcg acgatgacgc ccggagcatg
    28861 gcgcagtact tccagagcca gggcttcacc gtgcacagcg acatcgatcg gagcgccacg
    28921 gcggccgcga tcgaggccaa tctgacctgg ttgatgaacg ccgcggtggc gggcgacgag
    28981 atcgcgttct actactcagg ccacggcacc acctaccagg ggtatggatc ctgcctcatc
    29041 tccaccgact tgtactacct gacctgggga tgggtgatgc agttcatcaa cggcgccaac
    29101 tgcacgaaga agatgatccc gatggatgcc tgcaaactcg gcagcttcca caccggcgtc
    29161 ccgagcggga tgatcatggc cacggcgtcg accagcggct attcctatga cgcccccgac
    29221 ctgggccacg gcgcctggac gtactacttc ctggaagcca cggaaacgat ggtctacggc
    29281 gaggagatct gcacgtatgc caacagcggc atgaaggcct gggcggccct ctaccgtctg
    29341 aaggtagcgc cgaagatgac cgatgcgtac agcggcaagc tcgacatcta gagtttcaag
    29401 tctctgaccg ccaacacaaa gcccatctcg caggagatgg gctttctcat atcagcttgg
    29461 gcgggcaggg tcaaccgcgt cccggctgga ccagccgcgc ccgggcctgc accgcgatct
    29521 ccgacactgc ctcgatcgcc cggtcgatgt gggcctcggt gttgaacggc ccaatggcga
    29581 accggacact cccgtggcgc gccatcgtcc cgagttgctt gtggacgagc ggcgcgcagt
    29641 ggaggcccgt ccgcgtggcg atgccgaaat cgacgtcgag catgatcccc acgtcccccg
    29701 cctcgaaccc ctccacgttc atcgacacgg tcgacaggtg gttctccagg ctcgcgccgc
    29761 agtagacggt cacgccctcg atctcgcgga agccgtcgac caatttctgc gccagcttca
    29821 tctcgcgggc atggatggcc tcgacgcccc cctgctcggc gatccagtcc tgccccgccc
    29881 agagcgaagc cacgccgacc atgttcggcg tgccgtactc catgcggtag gggtattcgt
    29941 ccaaatggta ggggtacgcc gaccgcacgc cggtcccgcc gctccgggtg tggcggatgt
    30001 cgacgtggct gcgcacgcac ataccgccga tgccggttga ccccatgaga cccttgtggc
    30061 cggtgaacgc gaggacatcg acattcatgc gccgcatgtc gatcggcacc acgccggcgg
    30121 tctgggagac gtcgatcgcc aacagcacgc cggcgtcctt gcacacgcgc ccgatctcgg
    30181 cgaccggctg gatcgtgccg atgacgttcg atccgtggtt gactatcacc agccgggtgt
    30241 tggcgcggat cgcctcccgg atcgcatccg gctccacgaa cccctccgcg ttgaactcga
    30301 cgaaggtagc ctcgaccccg tgatcccgca ccaagtggtt gaccggccgg atgaccgaat
    30361 tgtgctcgag attggtcgtg accacgtggt cgcccggcgc gagcagcccc tggatgatga
    30421 ggttgagggc gtcggtcgcg ttgtagccga agcacagccg ctgcggcgtc tcggggtcgc
    30481 cgccgaagaa tgcagtcagg cgcttgcgaa gatcctcgag aatgttcccc gcttcgatcg
    30541 ccttgtcgaa accggaccgt cccgggttga ctccgcaact gcggtagaaa tcgaccatga
    30601 atttgtacac attgtccggt ttgggccacg aggttgccgc attatcgagg taaatcaagt
    30661 cgtccactgg gggcctctta catcacgggt ttctggcggg cctctccacg gccggccaat
    30721 ttgtacaaaa gactccgatt tgtcctctcc agtatactac attgcccgtc tttgtcaacc
    30781 gcgagcttcc ccccgcccgt ccggctgtcc ggtcacccgc tgcacgagcg ccgcaccgct
    30841 tcctccagcc ggtcaagctg tgcgtcgcgc gcctccgggg taactgtcca gacccgcacc
    30901 gcgggatgag ctttgacccc agcgatgaac gccgacccgg ccagagcgac cgtcgccacc
    30961 agcgggcgcg aggagtcaag gagcgtggtc accagagagc ggaacgcggg cgagagacac
    31021 tccatcttgc cgatttcatc gatcaagacc ggggtgtgca ggtcgtgcgg aaattccatg
    31081 gcggccagga aacgctccag tgcggcaatg tcaactccat atttcccaac gcgatatggt
    31141 gacgaaacat cgaccgtggc cagccggctc cgctgccgtc catcgagcgt cgccagcgcg
    31201 aagccgaccc gcaccccgcg ctcgcggatc tcctccgtga caaaccccgc cgggtgaaga
    31261 tccgccagcc gctccgccag gcgcatgatg agggtcgtct tgccgactcc cggccggccg
    31321 gtaacgagaa tcttgccact tgcttcgtga gtatccatat ccgtatggta cgtggacggt
    31381 tgtgacgacg gaacaactct ggcgccgccg ccccggcggt agtctacgac cccccgccgg
    31441 ctccggcaat cgaattgtgc gagaaaagga ctcatcgacc gtgcacaaga ggtttcttca
    31501 agagtgccga aaagccgttc acagtctcga ggcggttctg ccggaacatc tttcgcccga
    31561 aaccaggacc cgcgtggcgc tcgatctggt gctccgcatg ctgtttctcc gcttggccga
    31621 aaccgccggc caccggactg aacggccgga ccgcttcacg gaggtcctct acggatccgc
    31681 cgaggtccgg ggtccgctgt ccgtggtgcg acggcttctt cctcttccaa ctcaggtagc
    31741 ctgtccgggc gcgccaccgg tatcggatga ggctctgcgg ccgctgctgg gggaaaccgg
    31801 ctttctggac cgctatgcgt tctcggccca ggaggacccc ggccacgact cggtgatact
    31861 cggcccggcg gtgttggccg agctgatcga gacccttgcc cccggccggc gcgatcgggg
    31921 agcgtttttc acgccgcccg aggttgtcga cttcgtcgtc cgagcggcgc tcatggagtg
    31981 gcttggggac catcccgagc tgatcatcga cagcgacgga tcccgcatcc ctcccgcgcg
    32041 gcgagcggag atcatcgggc gcctcgccga tttgaccgtg cttgatccgg cctgtggggg
    32101 cggagccttt ctccttggga cgcagcgtat ccttcgcagc ctgggttccg ctctggatgc
    32161 ccccgagctg cccactgcgc gcaggctgta cggcgccgac attgaccccc aggcagtcgg
    32221 ggtcgcgcga ctccgtctct ggctctcctc tccccggccc acgcccggcc cggacgttga
    32281 ccttctgatc tcgcaggcag tggtggccga cacgctctcc gctgatcctc cttaccgaga
    32341 tcgtcgctgc tggcacttgg tgctgaccaa cccgccgtac ggggtgaaag tacctccggc
    32401 gacccgcgac cggatcatcg atccgcgacg agagggggcg cagtcccgcg atgcgtacgg
    32461 gttgttcctc gcgaacgctc cgcgcttgct gaccgagggc ggggtggcgg gcttggtggt
    32521 cgccgacacc tggcgaacga tcgccagcca tctgccgctc cggcggcggt tactggccca
    32581 ggttcagatt cgccgcatag tcgacctccc ggccggcacc ttcaaagcga ccgtgggagc
    32641 cgagatcgtg attttgcgga acgcgccgcc ggatccccgc acgccgctgc aggcagtcga
    32701 ccttcgcgcc ggggccgaga ccggactggc ggagaggttg cgggatctct tgcgagcgcc
    32761 ccatccttcc ggcgcccccg cagcgaccgg ccggtatgtc tacccgcagt cccggtgccg
    32821 tgcgcaccgt cgccatccgg tgtttatcgc gtctcccgct ctctttcgat tcctgatgga
    32881 tccgtcgatg atgcggatcg gggagatcgc cgacgtacgc cacggcctca ccaccggcga
    32941 cacgcgccgc ttcatttacc ggcggccggg cgcccccggc cggtacccga tcgccgaccc
    33001 ggagcggatt gtcgaaggcg aggacttggc ggaatggctg accgaggacg aacggcagaa
    33061 cggattcaat cccgaccggc tggacgggcg ctacctggtc ccctgcgaca aggggggggc
    33121 gagccggacc gccgatggtt ggcttccctg ctaccaggtg cccacggatt actacattga
    33181 ctggcgggcc gagagtgtcg ccgcgatgaa acggttgccg ggatttcggc atgacggacg
    33241 ggagtactat tttcggcgcg gcgtcacttt ctcccacacc ggccagtatg cgcccacctt
    33301 tcggttcggc gcgggcggcg tcttcgacac agccgggtcc actctcttcc cccgcgggca
    33361 cgacctccgc ctcctgttgg ccctcctgaa ttccagtatg gtgcgttacc tgttcaagaa
    33421 ctatctcaac cactcggtca acgcggccga gaacccgctc aaagagattc ccctcccgcg
    33481 ccccgatgcg ggcgccgagc gggatttgat cctgctggtc gaccggatcg ccgcccggca
    33541 acggaccgac cgctactacc ctttccaccg acacgagcag cgggagatcg acgccctcgt
    33601 tttcgggcta tacggtttgg cggaggagga gatcgaagag gtcgagcgct ggtgtgtgcg
    33661 ccggtatccg gccctgcagg gaccccgccg ccgtgcgcgg tccaggcagt aaccccttga
    33721 cagaaacaca attccggtgc taattgggga agaagcaggg atccaggttt tgcgggcgac
    33781 ccccgaccac ttcgccgtct gcttcgcagt cattgaggaa cgaccacacg aatcggaact
    33841 ccggagtgtg cgatcccgca acaggccttt tcgtcacgac aggcgttcgc cggaatcccc
    33901 ctccggtcgc gggtgaatca gcgcatgatt atccaagaca gctatacacc aagcaacgga
    33961 gtcgtctcat gaacaagctt catttcgtcc tccttatgat cctggcgacg gccggggtca
    34021 caccggccgc cgttcccacc caggtcagct ttcagggggt cctgacgaat gcctcgggca
    34081 atcccgtggc ggatggatcc tacggcgtcg tgttccgcat ctatgcgacg gatgtcacgc
    34141 cggtggcgct ctggaccgag gggcagacgg tcacgacatc ggggggccgc tttacggctc
    34201 ggctgggatc ggtgacaccg ctcacggcga gcatattcca gtcttcatcg cggtatctcg
    34261 ggctcacggt cggcgccgac ccggagattt cgccgcggac gaggttcctg accgtcccgt
    34321 acgccttcag cgtcgcgact gtcgacagcg ccgcgggcgg ccagatcaca tccggcgtcg
    34381 gcatcggcgt cgcttccggc ccataccggc tgcacgtgaa ttcctccgat tcggtggtca
    34441 gccgcataac cggcgacttt gacggtccat cgggcgcgat tttcaaggcc gaatatctcg
    34501 gccccacttc gtcggactat gacgcctatg cggtccgcgg gcttgccctg cagaacacgt
    34561 cgtccgagta cggcgtgggc gggcgctttg agggtggcta caacggtgtg gtcggcgtcg
    34621 ggtacagcgc cggcgtcatc ggcaccggcg ccgatgccaa cggttcatgt atgggggtct
    34681 ccggcagcac cgtgtccacc aacatcggcg atgcctacgg cgtccggggc acagccagcg
    34741 tcaccggcga cgccaacggc tacggcgtgt acggcatcgt caccggcacc actaccggcg
    34801 ttcgctatgc cgggtacttc gcgggcaacg ttcacgtcaa cggcacgctc tccaaggcag
    34861 ccggctcgtt caagatcgac cacccgctcg atccggcgaa caagtacctc taccattcgt
    34921 ttgtcgaatc ccccgacatg atgaacgtct acaacggcaa tgtcgtgttg gacggcgccg
    34981 gggaagccac ggtgagtctg ccggactatt ttgaggttct caaccgcgat ttccgctacc
    35041 agctcaccgc catcggcgcc cccgcgccga acctccatgt ggccgagaaa atcaacggcg
    35101 gccggttccg gatcgccggc ggcgcgccgg ggatggaggt ctcgtggatg gtgaccggca
    35161 ttcgacagga tgcgttcgcc aacgcccacc gcatcccggt cgaggagaac aagggcgcgg
    35221 aggaacgcgg gcggtatctc tatccggagg cgctcggcaa gaccgcggcc gagggtatcg
    35281 actccgcccg cgagcgcgcc gtggaccagc gcggcgacca ggcagaagac taactcggct
    35341 ctcccccggg tccgggccga tcgggagacc atcgttcttc gaggggcatc cgtttccatc
    35401 ggatgccctt cgcgcattac gaaggcgagt cggcctcgtg cgcccgctgc cccagggcgc
    35461 gggaactctg cgccggccga cgcagggtca gacggagggc ggtgaacccg aagaccgcgc
    35521 tgagcgtcga cgcgatcaga atccccgact tcgcgtcgat cagcagttgc ccctccaggc
    35581 tgagcgaagc cacaaagagc gacatcgtga agccgatccc ggcgagacag cccgccccga
    35641 tcagcgctcc ccaaccgacg ccgctcggcg gttcggccac tctgagaagc gtaaccagtc
    35701 ccagggcggc cagcaccccg gccggtttac cgagcaccag tccgaccgca atggccgagg
    35761 caatcgggct ggtgagcgat tcgatggcga aggggacgcc ggcattcgcc agcgcaaaca
    35821 ccggcatgat caagaaggcc gaccacgggt gaaccgccgt ttcgagacgt tccagcggcg
    35881 agacggcctc gcgcgaggca aaggccgcct cttccagcgc gtcgtgcaat tcggggcctt
    35941 ccggcgatcc cctcagccgg ttgcggatgc ggtcaaacat cgccgccagc gattccgccg
    36001 acagcagcgg ccgggccggc gtgagaaacc cgagcgcggc cccggagacg gtggggtgga
    36061 ttcccgattt gagagtgcac agccagacgc ccacgcctgc caatccatag gcggccacgg
    36121 cccgcacgcc cgcctgtttc atggccgcca cgacgccgaa cccggccagc gctcccagga
    36181 gccagccgaa tttgatcgcc gatgaataga agagggcgat caccagcacg gcgatgagat
    36241 cgtcgataat cgccagcgtc aggagaaaca ctttcaggct gcggggaacc cggtcgccga
    36301 ggagggcgag gaggccgacg acgaaggcga tgtcggtggc cattgggatc gcccatccgg
    36361 actggcccgg ctggccgtgc tgcagggcga agaacaaaat ggccggggct gccgctcccc
    36421 ccaccgccgc ggccgtcggc gcgatcaccc gccgcgcctc gcgcaactcg ccgctcacca
    36481 gctcccgctt gatctccagc cccacgagga aaaagaagac cgtcatcagc ccgtcgttaa
    36541 cccagtacca gagcggatag gagagagaga agcggccggc gccgaccgtg aggacggtgt
    36601 tccagaagcg catccacgaa tcggccaggg gcgagttggc ggcggccacg gctgccgccg
    36661 tgcacaccag gagcacgatc ccgcttgagg attcggtgtg gaggaagcgc gccagcgggc
    36721 gggtgagccg cgacagcggc cggtcggtcg tctctctcat ggatcctctc ctctgagggt
    36781 tcgtaccgat aataccgcac gatcccggcg cgggcaagcc ggttcggcgc ccggccggga
    36841 gaatcgtcac cggcccggcg cgaacgggaa gggagttgac gagcgggcac aaaaaaaccg
    36901 gggcggctgg ccgccccggc gcataatctc tgccgacaac ccgcacctag aagaacttca
    36961 ggccaagggt caccgggatg aacgacgacg actcgccatc ggtgtctacc gtgacgtagc
    37021 ggacctgccc gaagaagctg aacatcggac cggttttcag ctcgacgccg ccgccgaggt
    37081 tcaggtagag gtttgtctcg gcgtcggggt tgagctcgtt gaggccggcg accaggtcac
    37141 ccgtgccttc aagatcggag gctgagatgc gggcaaaacc gacgccgccc aggaggaacg
    37201 gcttgatcgg cgccgccggc agaccgaacg agtaccgcgc atcggcgccg tacatccaca
    37261 tattattgtg gccgccggtc acccccatgt cctccatgtc ctgtgtcaca tcgagcccga
    37321 accggtggta ctcaaccttg ccgaccacct gcaagctcgg catcacggcc agacccagac
    37381 cgaccgagcc gtggaaaccg gtgttgtagg cctccgagaa ctcctccggc gaattcggga
    37441 tggagatggc gccgccgaca tacaggctga tcgggttctc gacctgagcc gagacgccgg
    37501 ccgacataag caggagagcc gcaactgtaa tcaacgcttt cttcatgtcc tactccttac
    37561 gctttgttgc ctttagtgtg gtcattgcat agatcggccg attcgttccg gaacttgact
    37621 cccggccgag aaatttctcc ccccgcaagt cactgccgca gaacatatta tcggtaagac
    37681 gaggggccgc cactgaccgg gaaagcgggg gaaaggggat gcgcgcacct tggctacggg
    37741 gaatcgggtt gaggtcttgc ggacggatcg gcgccgcggg cgcgcctcac ggcagggtgg
    37801 cgtaggcggt ggtgtccatc cggttggcga cgaaccgata gcccgcggcc gaaagtttcc
    37861 ggattgcggc ggcgtagaat gcaccggtca tgtggagatt ctcgaagcga atgatccgcg
    37921 gatgatggcg cgcaagatcg aggctgtcga ggagttcgaa gtcgtaacct tccacgtcga
    37981 ttttcagcac gtcgggcggg ccgtcgacaa gcgccagcac ttcgtccagg ctcacgcatt
    38041 ccacggccag ctcctgaacc gcctccgggc cgagccggag cttgcgggcg tggttgagaa
    38101 catgccggcg gctgaaactg gccatgccgt tcgtggtcgc ccagagcggc agatcgcttc
    38161 tttggcgcag cacgtccggg gcgaatcggt agagcgtcat cgatttccgc tcgcggtgca
    38221 cggccttgtt gagcagcgcc acccccggat atgcggcgaa attgtgggcc agactcgcga
    38281 agacatcggg ctgcggttcg accaccacgc ctgccgcctg gaactcccgc acgtaggcga
    38341 tgaggtcatc agggcggctg acgccatcgt tggcgccgat ttgcacgaac ctgaaactcc
    38401 cggcgcggcc gtcgatttgg gcgcgcacga ggaagtcgaa gtcgagggcg agccgcgtgt
    38461 gcgaggcaag aaccgccggc atcgaccgct ccactaccag tccccgcgag gcgagaaacc
    38521 tgtttgctgc gctgcgaaaa ctcacatggc ctctttgcgt cgagagcgat cagagagtca
    38581 ccctgcgctt tcccgtatgg tacgtcggcg ccggccgatt ttcaactcaa attctcccgg
    38641 aaggccatct ccggcggccc ggagcctgcg gcctgaacgg ggagcggcta ggcctgcgac
    38701 gtgtacggga atatgaacgg cgcgccggcg aatgggagat cgcccatgac gtgcgtcccg
    38761 ttaccctcca cttccaccaa gtcgacatcc cccagatgca gcttgtcggt catgaaaaga
    38821 aactcgacag tctctccgtc cgccgcgccg aggtaccggt agatgtcctc gaacgggcgc
    38881 attgatggcg ccacgatgtc gaacacagtc aggatgccgc cggcccgccg ggccaggacg
    38941 gccgccccca gatcggggag gtataagatg taacgccgga gaccatacag acaatggtac
    39001 atgaacaggc gggcgttcag gacgccgagt tcatccgaca ccggcgtccg ggcggcggcc
    39061 agacggtaca gcagcgcgag gtgctcggga tcctgcgggt ccagcgggac agcgccgggg
    39121 cgcgcccgca ggcctggcag aaagacgcgc gccttgtatt ccgccgccgg ccgaaaaccg
    39181 caggaggcat agagcgggaa agcttcggca tccgcaaaca gaaagaagaa gtcgtggtcc
    39241 cggcgcgccc acgcgaccgc cgtctgcagc aactgccggc tcagacctct ccggcgaaac
    39301 tccgggagcg tccccaccgc ggagatctgc gccacccggc gccgtgttcc ccggacgatc
    39361 atgtccaggg agtagacgca gacgttggag accagcgtgc cgccgtggaa atacgaaaac
    39421 ggccggtacc gcgcatccca gaatcccaac cggtcccaga gggacaaatc gaggccgaac
    39481 agcccggcca agaagcgtcg gtactcttcc ttcagttcgg gggcgtccca gtaatcctcg
    39541 cagtacgaca gatccatatt tgtctccgcg gtttgcgctg ccgacggcaa aacatccgga
    39601 ccgatataac tacatgccca gaagcacaaa cgctccccac agcgccgggt cgatggcggt
    39661 cgccgcgatt tcggcctgcg ccgtgcggta ggacgcaacc acgtcatcgg tcgcccgcag
    39721 gcgggcgtag aaccggctca tgaacgcatg gctcacctgg tcggacaccg gccacagggt
    39781 cgccaggaca tagcgggcgc ccgcctggta gaatgctttg gcgaggctga agctgttgcc
    39841 gtagtagagg ccggtcgcgg ccgtctgaca gccggagagg gccaccaggt cggcgcggat
    39901 gccggccgcg aacaggtcaa acgggaagaa cggcccgtcg gccagtacca cccgcgaaaa
    39961 cagcgggttc tccgacgacc gcgcggcgtg ggtggcgatg tggatgaacc catcgcacac
    40021 acccagcgcc tccttgaaag ccgtcgaatc cgcccgctcg tcgacataga gaccggcggc
    40081 ctcaaactcc cgccgcacgg cgccggcctc gagttccacc gccggcagac ggtcggagag
    40141 gagggcgaag acggcattgc gccgagccgc gaaaggaacc ctcccccgcc ccgcccgcgc
    40201 tcccggcggt acgccatcgc gggcgagatc gtccgggttg acaatgagcc gaaggtcgcg
    40261 ccgccgacag agcggcagat gcgcccggtc cagcagcgcc ccgaagggta cctgcgcaaa
    40321 gatgccgtcg gtcaaaatga tgacgcggct ctggtcgtgg tcccgcagga gaggggcgac
    40381 cagggtttcg tggagatcgg cgagatagaa atcggccgcc ccggggaacc gtcgttcgcc
    40441 gcggccgctg atgacggccc gctcgaggac gaaatagagt tcccagacct ggcggcggag
    40501 acgctcggcg tcgatcgcca gggggatgaa gtcaaccgtc ccgcgcgtcg cgcggaaggc
    40561 gcccggcgga ccgccgggag gcgcgacgaa actgaccagg agatcgtcgc cgcccagccg
    40621 ttcctgcacg tcctcgcgcg ggcccgcggc gcggccgcgg ccccgggccg ggtagagatg
    40681 ggcgcggatg cgctgttcgg ccgaccacag ccgctgctct acggcggact gctgtgcgag
    40741 gctgtcggat ccccggtgcg tcttctccgg gaaacgcccg aaccgcttga gggtcgcccg
    40801 gaggcgctcc cgttccgcca gcagcgccgc gggcacgcgg gcatgcacct cggccgccag
    40861 cggatagcgc cggttgagca cctccagagc ccgcaggttg gcccgaaagg attcatcggg
    40921 gcggtccatc tgcaggaggc actccaccgc cccggcgtag gccgtgagct tgtcggcgac
    40981 aaagaagaac cggatctcgt ccgggtacag cccggccagc atcgtctcga ccatgtcgat
    41041 ggcccggcgg taggcgtcga gagcgccggc ggcgtcgccg ccggcgcggc ggaggcggcc
    41101 gacggccgtc tgcacgaggt aggactggta gctcacgagc gggcggcgca gcaggcggcg
    41161 ggccagcgcc gccgcctctt ttcggcggcc ggccgcgcaa agggcctcga ggtggacgat
    41221 ctcggcgtcg gtcccccggc gccggtcgcc gctgcgcgtg aagaggcggc gcgattccgc
    41281 cgcggctctc agcgccgcgc ccgaacggcc cagttgcgaa ctcagcttgc tgcgcgccaa
    41341 cagcaccatc cccagccaga gatcgttccg ctcccggcgg aacagccggg ccgcctcgtt
    41401 cagcgcctcg gcggcgagac ggaaatcgcc gaggctgagc cgcgagagcg ccacgaagta
    41461 gttcgccttc gcctgctcat agcggagacc gaggcggcga aactgggcga tcacctcctc
    41521 ccccgcggcg atcgcggtcc cgaactgatt gaggtggatg ttgagttcca ccagatcgag
    41581 gcgtgtgacc gccatggagc gcgtgtcccc gaggcgctcg aacgcgacgt acacgttttc
    41641 gaattcgcgg agcgcctcgg tgaaccggtc gcccagaaag agcaggtagg cgagcgagta
    41701 ttgggctttg cggtagagga cctccagacc ggaggcgcga tagatctccg cggcccgccg
    41761 atagagcggc gccgcgaggt gcggttcatt ccggttggcg tagatgttgg cccggttgta
    41821 ctcgacggtg gcgagcggcg cgccgccgcg ggtctcgaag atggcgcggg ccttatcgta
    41881 gtagaccagc gcgaggcggt tctgatcgag acggtggtag acgttgccca tgttgaccag
    41941 aacgcccgcg gccgccgccg ccatcccgcg gcgccggaaa tatccgagcg agcgccgccc
    42001 cgccgccagg gccgagcggt aacgcccgag gtacatcagc acttccgtca tcccctgccc
    42061 caggcgggcg gaggccaggt agttgcgctc ccggcgatag cggcggtcag cctcgcggta
    42121 ccgggccagc gccgcccggt accggccgcg atggtgcgac aggcgcgcgt cgagggccac
    42181 aatgtgcgga cggtgcttcg ccggcatgtg ctcgtacagc ggctcgattc gccggaggaa
    42241 ctcctcggcc gcgcggaggt cggtcccgag gagcgtattc agttgtcggc tgagggcggc
    42301 caggagtttc tcatagtcgg cctcaaactc cccctccacg agccggcgga gatcctcggc
    42361 cgacctgacg gccggcaatg tggaatcatc gtacatagca gcgtcgccca agagaccgtt
    42421 gccctaagtt aacgcctgga agtggcgggg caaggagttt gtcggccggt cgggcgcggc
    42481 cgcgcggccc cccggcacaa gagtgcttga caaaaggcgt ctccgtgggt ctttttgcag
    42541 catgatgcgg cggggacaac atggcggatg gtaagcgggc ctctgtcgaa gaactcgtcg
    42601 gcgcctgccg ggagggagcg gagtgggcat ggcgggagct gctcggccgg gtgtctccgc
    42661 tggtgctcgc cctgtgccgg gacagccgcc tctccgcgga ggagtccttg gacattttcg
    42721 gccaggtctg ctatcttctg gtgcgcaaca tcgaccgcat ccgcaacccc cgcaggacgt
    42781 acgcgttcgt cgccacgatc acccgccgcc agatctacaa ccggttccac gaggaccgcg
    42841 gactggagtt tgtcgacacc gatcggctgg gggagacagt cgaggacccg gccggcggcc
    42901 cggaccggaa gttcgaggag acggagcagc gcctgctgct gacagaggcc attgccatgc
    42961 tgccgccgcg ggactacgaa ttgctcacgg cgctgttctt cgacgaggac aagccgagct
    43021 acgaggagat cggggcgcgc ctggggatgc cggtcccgtc gatcggaccg acgcgggcac
    43081 gcgcgctaca gcggctcgag cggctgctcg tccggatggg gtataaattc gacgtatttt
    43141 aggcgcggaa aagcgggact atgagatgag gaactggaac catgtcatcg aagcgcgata
    43201 aagtcgaacg cctgcagcag tatctggacg gacgcctgga tccggaggag gcggtgcgcc
    43261 tggaggaaca cctcgccgcc gatccccggc tccgccggat gctcgatctg cttcgggcga
    43321 tgcgccggac acagcctggt gacggcgctc tcgaatccgc tgcccgggga ctcatcgacc
    43381 gcgtggtgac cgacctgcac cgccggaaag acagcccgca ccgcggcctc ctgacttttg
    43441 actcggggct tctccccctg ccggccggga tacgcccggc caccctcgac agccgccgcc
    43501 tccggtatgc ggcggaaggc ctccggctcg aactgtcggt ctacccggcc tctcccggga
    43561 catttgaagt catcggccag atcgaggggt cccgtcgggg tgcggcgctg gaggtctctc
    43621 tgcgccaggg ccggcagacc ctcagggagc ggaccaaccc cgttcacgtg ttccacttcg
    43681 cccgggttcc ggaaggcgcc cacgacatga ccgtcgaaga tcccgacggc gggcggttcg
    43741 agttccttct ggagttgtag ccgggcggac aggttctcgc cgcaacgaga tctctccgcc
    43801 gcgtaaccgg ccggctcacc ggccctgaca tccgccgatc tccctgtact gcagcggatc
    43861 ctgtcaagga accatggaac gctcaccgcc tctggacaac ccgcctcttt gcctccatgt
    43921 gagacaccga caccggtcgg gtaccgcccg tactcgcatg cggatcccga ctgtccctcg
    43981 tgaaacgtgc aacaatgaaa cagggggtag gtcccgtgac aactcgaaac cgtcttctcg
    44041 cgttcgttct cctcttgctg gtcggatcgg cctgtccgcg gggcgccgcc gtggccgagg
    44101 cgcttcccgg ccaggctctc gtccgcgtcg acccggacca gaacctcggc tgcattctga
    44161 ccgggacagg catcgtcatc gccgacagca ttcccggctc gcggctgtac ctggtgcggt
    44221 tctcaccgac cgtgtccttc gacaccaagc ggcaggatct cgcgggccgg ccgggaatca
    44281 gcgccgtctg ggccaatgag ccgatgcacc tggtggagac ccaccagatg agcatcggcg
    44341 ttccggaaga cgaagttccc gttttcgtgc ggggggtgga accgcagaac tactatgccc
    44401 agccggccgc ctattcgatc gggctcgact cggccctcct cgtctcgcgg ggcgcgggcg
    44461 tgcgcatcgc ggtcatcgac ggcggcgtgg actgcagcca tccgcttttc gccgacctga
    44521 acgactctct ggcccgcgat tttgtggaca acgacgccga ccccagcgag ggtccgggga
    44581 cgctgcgcgg gcacggcacg tttgtggccg gtctcatccg gctggccgcc ccggaggccg
    44641 agttaatggt ggtgcgggcg ttcaccggcg atggcgtgag cacgacgttc caggtggcgc
    44701 aggcgctgga ttgggccgtg agccatggcg cgggcgtcgt caacatgagt ttcggcaccg
    44761 ccgccgacaa cggcgtcctc gcccaggcct gcgcgcgggc ggtccaggcc ggcgccgtcc
    44821 tcgccgcctc ggtgggcaac gacggcatcg tgacccccat gtatcccgcc tcgtacccgt
    44881 acgtcctggc ggtgacggcg attgatacgc tcgacctcgc tgcctcgttt tccaacggca
    44941 gccccgaggc cgacgtgtgc gccccgggag tcgacatcta cagcgccctt ccggctccct
    45001 attactgggg gacctggagc gggacgtcgt tctcgacgcc gctggtgacg gcggtcgccg
    45061 ggttggtgcg cgcggtccgc ccgaccctca cgccccagga aatggagacg cacattcgga
    45121 gcacggcggc ccgcgaactg gcctggggga cggtcgcgcc ccccgacttg ttctacgggt
    45181 acggccgcct cgatgccttc acggcggtgc tcctgttccg gctcggcgac atggataact
    45241 ccggggtggt ggacgtggtg gacctgaaca cggtgaccgc catggcgacc accggagcca
    45301 cgccgccgaa cagcatccgg cggcaggggg acatcaacag cgacggcacg gtcgacgccg
    45361 aggacgtggt ggagctctcc gatgagctct tcggcggcga ccagtaggcc atccgcttgc
    45421 cggcccaagg ggatctcgaa acggcgtcgt tcgcacggcg ctgttttcgt gcgatagacc
    45481 gggggcgccg cttttttgat gtatttttcg tcccccttgc gcgctctatc tatcagcagc
    45541 aagacatcgc ccgttacggc aggtggctcc ctctctttcg gcggatgtat ccatagccac
    45601 ctgtcgaggg cgataaagca atgcggggga gggggggttt tttttgcggg gagggcgcgc
    45661 cggctcagga acagaccagc ggtcgggggg gcgacgggca ccgcccccgg atcggaacgc
    45721 gacgcagttc cggccgtcgc gtggctgtcg cagccttcga tgccctgccc ccagggcttc
    45781 tataccctgt cagtacccgg tgcgggccgc tcggtcccac cggggatttt ttgccccttc
    45841 gcgaaacctt acctacatag attcgtttta tctatattca gacaacgtgt ggcgccgccg
    45901 ggggcgccgg gccgcccgga cggaggctat tcggacaagg acattctata tcggccgccg
    45961 gggtggcggt taaaccaaca agggtgcgta agactttatg aaaaaacggc ttatcgggcg
    46021 ggcgctggtg tcgacagcgg tcctcatgct ccccgtcctg acatcggcgc agggggcgcc
    46081 gccgactctg gtcgtcaccg actcggtgcg gcagatggag ttccacgacc aaatctcgct
    46141 taccggccgg accgaagcgt gggtcaccag ccgggtcgtc gccgaggtga gcggccgggt
    46201 cgaggcgatc gcggccggcg aaggggcggc ggttcagaaa gaccagacgc tggtcatgat
    46261 cgatggcgag cgcatggggc tggcgctgca ggccaaggcc gcggaagcgg agcaggcccg
    46321 cgtgcgggcc gcgctggcgg ccgacaacct gcgccgcgcg gaggatctct tcagccgcac
    46381 tctgatctcc acggccgcta ttgactcggc ccgcgccggg gccaccatgg ccgagcaaag
    46441 ccaccggcaa ctcgaggcgg agcgacggcg gctcgaagtc gagcacgaca actgcacgat
    46501 ccgggcgccg ttctccggct atacgggccg caagctggtc gacgtcggcg agtgggtgag
    46561 cccgggaacg ccggtgttcg aactggtcga cgtctcaacc atccgcgttc gggtcgatct
    46621 gcccgagcgt tacttcggcc tgctctcggt cggcagtcca gcgaccatcg gcctgagcga
    46681 gcgcggcggc gaggcggtgg ccgggcgtgt ggtcgggatt tccccgaatg cgtcggaaga
    46741 tactcacacg ttcccggtta tcgttgaggt gcccaaccgc gaggggcagc tcgccggcgg
    46801 catgctggtg cgggccaccc tctcgctgga caacaagttc accggactgg ccgtctccaa
    46861 agacgccatc atccgccagg gggcacagac tctcgtctac accgtgcacg aagggaaggc
    46921 ggcgccgatc ccggtcgtca ctacctcgac caatggcaag atggtggcgg tgcggagcga
    46981 ggctctccgg gcggggacac cggtggtcgt gcgggggaac gagcgcattt acccgggcgc
    47041 ggcggttatg atagcggacg gcagccgtca ggcgaatccg cccaaagccg cgccccaaac
    47101 caaccagtaa gcgccgcccg gcggcaggag tcataccccc gtgagcctga ttacctcctc
    47161 gatccgattc ccggtcaccg tcatcgtcgg cgtgctcatc gttctcatcg gcggcatcgt
    47221 ggcgctgacc cgcgtcccgg tgcaactcac gcccgaggtg gaacgccccg tcgtaaccgt
    47281 caccaccacc tgggtcggtg cgtcgccggg cgaaattgaa aaagagatca tcgagaagca
    47341 ggaggagtat ctcaaatcgg tcgaggggct gctcgagatg agttcggaat cgcaggacag
    47401 ccgggggacg atcacgctgg agttcccggc gggcaccgac ctgacgggcg cggtcatccg
    47461 cgtgaccaac aaattgaacg aggtgccgtc ctacccggcc accgccgatc ggccggtggt
    47521 gaccacctcg gggcagttgg agggggccat cgcctggttc gccgtcaagc ccgattcggc
    47581 cggcgtctat gtcccgcatc agcaggcggt gatcgaggac atggtcaagc cgcgcctgga
    47641 gcgcgtcgaa ggagtggcgt cgatcaacat cttcggcgga ctcgagcggg aactgcacgt
    47701 gacgttcgac cccgaccgcc tggcctcgac cggaattgcg atcgaccagt tggccgaggc
    47761 gctccgcgcg gacaaccggg acatctccgg cggcgacttc gcggagggca agcgccgcta
    47821 cgtcgtccgg accatctcgc gctacgagac cccggagcag gtggagcaga cggtggtgac
    47881 ggtgcgcaac ggcgtgccga tccgcgtcgg cgacctggcg ggggtggagc tgacgtatca
    47941 gaagcctgcc gccatggtgc gccacctggg cgtgccctcg atcgctttca acgcccagcg
    48001 ccgtgtcggc gccaacgtga tggaggtgat ggacgggctc atggcgcaga tcgacatcat
    48061 caaccgcgag attctgcacc cgcgcggcat gcacatcgaa aacgtctacc gcgaaaccgt
    48121 ctacatcgac tcggccatcg acctcgtcct ctcgaacatc tacgtcggcg ggttgctcgc
    48181 catactcacg ctgttcatct tcctgcgctc cgcctcggcc gtgctggtga ttgcgctgtc
    48241 cattcccatt tcgatcatct cgaccttcct cgccatggcg ctcttcggcc ggaccatgaa
    48301 cgtgatttcg ctcgcgggca tggcgttcgc ggtcgggatg gtggtcgaca gcgccattgt
    48361 cgtgctcgag aatatctacc gccacatgca gctcggcaag tcgcggtggc gggccgcctc
    48421 cgatgccgcc cgcgaggtct ggggcgccct gctcgcctcg accgtcacga ccgtcgccgt
    48481 cttcatcccc atcatgttcg tcaaggagca ggccggccag ttgttccagg atatcgcgat
    48541 cgccatttcc acggctatca tcgtgtcgct cgcggtctcg gtcaccgtca tcccctcgct
    48601 gtcggctcgg atgctgaggg tgtcgaccaa actgaccgcg ggggatgaga cgcaagggtg
    48661 gctggcgcgc ctggccgcga gagtcgcgag tttcgtggac tacgtcaacg cccggggccg
    48721 ccgccggctg gtgaccatcg gcgccatcct gacggtggcc atggggctca cggtggcgct
    48781 cataccgccg agcgaatacc tccccacggg aaaccagaat ctcgtcttcg gcgtcatgct
    48841 ccccccgccc ggctacaacc tcgacgagat ggtccaggcc ggccagacca tcgaggcgca
    48901 gattcgtccc ctctgggagg ccgacgcgga gacggccgac actctgccgg gcggcggcat
    48961 cgacaacttc ttcttcgtgg cgctgccgaa ccaggcgttc atgggcatgc gcgggcgggc
    49021 gccgcagcgc ggagcggaac tggtcccggt ggccaaccag gccctgtggt ccatccccgg
    49081 cgcgatcggc ttcgccgccc aggcctcgct cttcggcggt gggttcgcgg gcacccggtc
    49141 ggtgcgcatc gacgtgaccg gtccggacct ccccaaagtc ctccagatcg cccagcaggt
    49201 ttttgtccag gtcccgcagg tgctgccggg agcgaacgcc cgccccatcc cggggctcga
    49261 cctcggcaac ccggaggtcc gcatctaccc cgaccgcgtc cgggccgccg acgtcggctt
    49321 tgacgcctcc gcgatcggcc gctccgtcaa tgccctcgtc gacggagcca ttgtcagcga
    49381 gtatttcctc gacggacgcg agatcgacct gctcgtgaag ggacgcgacg attggagccg
    49441 ccacacgcag gacatcgcct tgctgccgct cgccactccc accgggcggg tggtgacggt
    49501 cggcgacgtc gccgaggtgc gtcaggaaca gggtccggtc cagatcaacc atgtcgagcg
    49561 acagcggacc gtgtcgatcg aaacggtgct ccccgacggc atcgctctgg aggacgccgt
    49621 gcgccggctg gaggaacagg tggtgcagcc gatgcgcgcg caggggcaga tc
//
LOCUS       DAOPOE010000011        49660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_904_length_49660_cov_5.091684, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000011 DAOPOE010000000
VERSION     DAOPOE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 49660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49660
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_49660_cov_5.091684"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKM94_02820"
     CDS             <1..706
                     /locus_tag="PKM94_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD65598.1"
                     /translation="DRVQAVIERARTESRTTIVGAEAIEILCAYGIPAAEYRFASSER
                     EAAAIAKGMGLPVVMKVAAPAILHKTEVGGVMLDLRTEREVRDAFRELRKRVAAERAA
                     GETFAVALQQMVAGGVETVMGMTTDPSFGPLIMFGLGGIYVEIMKDVAFRINPLTTAD
                     VRDMMRSLKSYPLLKGYRGAPPVDLATVEDTLLKVSQLVKDFDAFSEVDINPFIASPE
                     RARCKAVDARFIMKEG"
     gene            816..1388
                     /locus_tag="PKM94_02825"
     CDS             816..1388
                     /locus_tag="PKM94_02825"
                     /inference="COORDINATES: protein motif:HMM:NF023511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65599.1"
                     /translation="MKLELKAVSVWTAVKIGFFLNLIMGFIMGLVAALFMAPLMAVMS
                     TMMPAGAGGSEFAAMPVGLLLVILPIGYAIFGAVFGTITFAIGAGVYNVTARILGGLE
                     ITYTVVEWGDPSGAAQAPPPFPRYPAPAPPYPAYPPPTGRPGPYSGSPMPPPPPPPEA
                     RPEPQAPHRPLPEAPPPGGPPEEPEKPTQP"
     gene            1398..2852
                     /gene="gltX"
                     /locus_tag="PKM94_02830"
     CDS             1398..2852
                     /gene="gltX"
                     /locus_tag="PKM94_02830"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOD65600.1"
                     /translation="MTDRSIRVRIAPSPTGYLHVGTARTAIFNWLFARHSGGKFLVRI
                     EDTDQARSQADLIAPILDAMRWLGLQWDEAITYQSQRLDTYNACARRILELGRGYRCF
                     CTPAELERGREEAKARKQPLRYDRRCLRLPPDEIARRVAAGMPYAIRILVPEGETRFH
                     DLVLGDLGRHSDDIEDMVIARSDGSATYNLAVVVDDHEMGITHVIRGNDHVTNTFKQI
                     HVYRALGWEIPQFGHLPLILRPDKKKVSKRLGDKDVNQYSREGILPEAMFNYLCLLGW
                     NPKTDREIYTREELIALFTPAYFNASNAVFDEEKLEAFNREHLLRRSNHDLATMAAPL
                     LVEAGLTTKYWLETRWKYLCEVVGLLKDRVRRVTDFAALGGYFFAFDYRYDAAAAAKH
                     FHGGAADLLARLADQLEPLAELTADNCERAVEAAAAAAGVKKASVIHPTRLAISGMPA
                     GPGLYEIMALLGRSAVLERLRKAIDYIHATPGAS"
     gene            2873..3373
                     /locus_tag="PKM94_02835"
     CDS             2873..3373
                     /locus_tag="PKM94_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD65601.1"
                     /translation="MAKTKEYIAETLKKAIKGEEDGYTFYDLLMERATNPDAKRKLKN
                     LRDDEVRHKHTLVEIFHKHIGPEIGDLPPKGINALAQVFRKGRLEERKSEMEFINLAI
                     EAELAATRYYQTERDLVDDPEFREVFDSLAEEEHRHYELLQAEKDAISGNYFWFGLDD
                     TSPLEH"
     gene            3430..3615
                     /locus_tag="PKM94_02840"
     CDS             3430..3615
                     /locus_tag="PKM94_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65602.1"
                     /translation="MQWECVICGLIHEDDEPPPVCPVCGAPGSRFTERYEPEDELDEA
                     REEDEGDDDDEYYGEFQ"
     gene            3633..4181
                     /locus_tag="PKM94_02845"
     CDS             3633..4181
                     /locus_tag="PKM94_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD65603.1"
                     /translation="MAAPERVKTRTRTLEVGDEAPDFTLPTHNEGDLNLAWYEGRRIV
                     VLAFYPGDWTPVCATQIPEYQGLIDEFARYEAQLLAISVDSRPCHLAWARSLGGLSFP
                     LMSDFWPHGGVAEKYGVLSERGFAERAVVVIDKRGIIRYIEVVPSSELPDNGKLFRCL
                     AELKREDESAAAAGDRGAGEDR"
     gene            4331..4627
                     /locus_tag="PKM94_02850"
     CDS             4331..4627
                     /locus_tag="PKM94_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD65604.1"
                     /translation="MAGLEDRRADFERHGARVLAVNPATLAEHRQWALLLGLHFPILS
                     DADWRVTRAFGCKTPFLLVVRRTVYALGRDGRVVFAARGHAAWDEVEAAIRAAE"
     gene            4650..5726
                     /locus_tag="PKM94_02855"
     CDS             4650..5726
                     /locus_tag="PKM94_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD65605.1"
                     /translation="MSEHEARHDSRDIYDVSFIGAGPVAMYGMYYAGLRMMRAKAIDM
                     LEEVGGGLMALYPEKYIYDVAGFPKVLAKDLVRQFEAQAAQYPTNTFCLGEKVTEVTR
                     EGDGVFRLTTDRQAHYTRTVVVCAGLGAYIPKRMDVPNVEKLEGRGVYYFCRRLRDFA
                     DKDVLIVGGGDAAFDYSMMLGPVARSVTHIHRNSFFSAHEDSVRKVKNSRVRLLYPFW
                     ELKELRGEDWVEGATIVQTRTGHEERLAVDAVVLNIGFLTNLGPIEGWGLELDKNAIR
                     VDTRMRTNVEGIYAAGDIVTYEGKLKLISTGCGEVAIAVNNAKAYLDPRAKVSPGHST
                     EKHEVVMRKLQQRQQQSRGEEDRE"
     gene            5731..6810
                     /gene="nadA"
                     /locus_tag="PKM94_02860"
     CDS             5731..6810
                     /gene="nadA"
                     /locus_tag="PKM94_02860"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOD65606.1"
                     /translation="MYFALPKEYREATVDDLQARAQAVRDRLGKRLLILTHHYQRVEV
                     AAFHDHLGDSYGLSKLAAAQREAEFIVFCGVHFMAESADVLTAPEQKVFLPNPLAGCP
                     MADMAEMADVLAAWECLEEFGGDRRVMPISYMNTAAGLKAFTGRHGGLICTSSNARAA
                     LTWAFGLREKVFFFPDEHLGYNTGIALGIAPEEMVLWDFKRDGGGLTRAQMEKAKIIL
                     WKGHCHVHTNFRAEHVEQVRRDYPGVKVVVHPECPHEVVRLADSVGSTSHIVDFCRKA
                     PAGSTIAIGTEINLIHRMALEHPDKRIFELSGQTCPVCANMYRTTLNDLVYTLENLDT
                     VKPIAVKEPIRSEARLALEKMLEIA"
     gene            6911..8257
                     /locus_tag="PKM94_02865"
     CDS             6911..8257
                     /locus_tag="PKM94_02865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOD65607.1"
                     /translation="MAVDVLVPQMGESVLEGTILEWKVKIGDRVKADQPLVELMTDKI
                     NIEIPSETDGILTHLYAKEGEVVPVGTRIATIDDGGAAAKTAKTSEERPQAAPAAVSA
                     PAAPPKPRPAVAGTAPAGQGKLSPKVRVMIREYSLDPATIVPTGKDGIVKVEDVLRAV
                     EERSLAQGFAAAPIPAPAPPESAAAGPRPETPPPAVPAAPKVTIEVPKFAPLPESQRE
                     RREPVTGIRKSISEHMVRSVQTSPHVTTFEELDLTDLAAFRNEIKTDFRATYGANITF
                     MPFIVKAVCVALKEYPKLNASMTEKEIIYKNYYNIGIAAAREEGLIVPVIKDADKKSV
                     VELAVEIAQLGDKAKRNRLVPDDVSGGTFSITNAGMFGATASTPIINQPQVAILGVHA
                     IVKRPWVVNGEIAIRDISSFGLSFDHRLIDGHTAVQFLHRIHELLADPRRLALHLR"
     gene            8259..8960
                     /gene="lipB"
                     /locus_tag="PKM94_02870"
     CDS             8259..8960
                     /gene="lipB"
                     /locus_tag="PKM94_02870"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOD65608.1"
                     /translation="MSVYTPRPYGWVLEVGRVEYERALAWQRGLVKMRREGMARDTIM
                     FVEHPAVCTVGRDGHEEHYRNISSAPVFVERGGDVTWHGPGQLVVYFIFNLARRGRDL
                     HAFMTAIQQGIVDALAEWEIAARLGEEYTGVWVGPKKIASIGVAVKKWITFHGAAINL
                     NTDLREFAQMEPCGLSADLMTSAAEQLGRPVDMQAFAQALLRGYAERFHTQFDPVALE
                     AIAEDLESQSGGYTI"
     gene            9001..9747
                     /locus_tag="PKM94_02875"
     CDS             9001..9747
                     /locus_tag="PKM94_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD65609.1"
                     /translation="MEFEGKVAFVSGGTRGIGLAVCRKLAARGAHIVLTYFRSRSNAQ
                     EAVAELEKFGVEATAIRANMGNMEQVADVLKTIREKYRKLDILVSNAALGTYTDMMNI
                     DRKDWEIAMHTNARAFLQLIQMGIPMMPRGSKVVALSSLGSIRYIPGYAAIGVSKAAV
                     ENMVRYAAIEMASREIYINCVSGGFIDTDALKVFPNYEEMKKQVTARTPFGRIGRPEE
                     VAEAVVFLCGDGASWITGQTLIVDGGYSLM"
     gene            10126..12669
                     /locus_tag="PKM94_02880"
     CDS             10126..12669
                     /locus_tag="PKM94_02880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOD65610.1"
                     /translation="MSEKGRSAGGGRRLYGAIRSVAAGLAAALAAPLARASEADIVVP
                     DLNTGDFFGMTGHNLLLWGIVVCLLGMVFGWYAYRGLRKLPVHQAMRDISELIYETCK
                     TYLQTQGKFIMLLWAFIGLIIIVYFGWLRPVGAGNLLIILLFSLVGIAGSYGVAWFGI
                     RINTFANSRSAFASLTGKAYPTFAIPLRAGISVGTLLISIELIIMLAILLFVPGHLAG
                     YCFIGFAVGESLGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGD
                     NAGDSVGPTADGFETYGVTGVALITFILLAVMNPAIQVQLLVWIFVMRVMMIVTSIAS
                     YWINGALARGRYGDQATFNFEAPLTSLVWITSIVSIAMTYVISYAMLAELGGGSLWWK
                     LATIISCGTLAGAVIPELVKIFTSTKSRHVKEVVTAAREGGASLNILSGFVAGNFSGY
                     WMGLSIIALMAIAWGMSTSGLGAIMAAAPVFAFGLVAFGFLGMGPVTIAVDSYGPVTD
                     NAQSVYELSTIETIPNIAQDIKREFGFTPDFEKAKLTLEENDGAGNTFKATAKPVLIG
                     TAVVGATTLIFSIILLLATEAAGLEGVLTLEQINQALMMAGYHFSLLNAPFLLGLIAG
                     GAVIYWFTGASTQAVSTGAYRAVEFIKANIRLDGTSQKASVKDSKRVVEICTQYAQKG
                     MFNIFIVVFFTTLAFACVDSYFFIGYLISIAIFGLYQAIFMANAGGAWDNAKKIVETD
                     LREKGTPLHAATVIGDTVGDPFKDTSSVAMNPVIKFTTLFGVLAVELAIELTQGTRVA
                     LAALFFAIAAAFVYRSFYRMRITTTGVQEPAKPNRKAAAGEPAEVRAAR"
     gene            12886..14610
                     /locus_tag="PKM94_02885"
     CDS             12886..14610
                     /locus_tag="PKM94_02885"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOD65611.1"
                     /translation="MKRFDMRVDPLTNEIYYAVPHKGHMLVSDPLLNKGYAFTRAERN
                     EFELLGLLADQVGTLEQQRTRNHNIIKSMNSDLDKYVELIGLLDRNETVFYSLLAHHI
                     EEMLPIVYTPTVGLGCLKRSHIIRRWRGIYVSPSNVEWMEQILRNVGLPNVSLVVATD
                     GERILGLGDLGADGMGIPIGKIALYVAAAGIHPASTLPVMIDVGTNNERLLNDPLYTG
                     YRQPRLTGDAYYEVIERFVQGLRRVFPRALLQWEDFGKQHAATLLERYHERILSFNDD
                     IQGTGATAAAVLRSAFRLTGRPAAEERIAIFGFGQAGCGVANALVTLLTLEGGLSTAE
                     ARRRIFAVDVNGLLLQGDPADAYQAEFRQPREVAADWPAPKDRAPNLQEVIRHGRITT
                     LIGLSGQKGAFTPEILAALAANAPRPVILALSNPTSCCETTPEAVVAATGGRGIIATG
                     SPSRPVTLPDGREMTVSQCNNLYVFPGMGLGAIVCQAQKVSHRMFHAATCAISDMVTE
                     EMRSTGCLLPALENAREISFAVALAVARQARDEGIGLAASDDRLAALIRGAMWEPKYY
                     PYRADMGH"
     gene            14939..15568
                     /locus_tag="PKM94_02890"
     CDS             14939..15568
                     /locus_tag="PKM94_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOD65612.1"
                     /translation="MLEKLLPRETSFFKFFEQTSGLAIEVCVELDNLVNSSNDVAGRV
                     ARIEQLEHDADEVTHQCIDALHATFITPIDRADIHRLIKRIDDIIDCVDSAAARFQLY
                     GLTVMRPEIRQLTQVLIASVRELDKAVRDLPALNRKAEAIQECCQAVYEAEREADDLL
                     RAALVRLFAEEKDVFLVIKWKEMFERVERATDRCQEVANIISGIVIEAS"
     gene            15573..16604
                     /locus_tag="PKM94_02895"
     CDS             15573..16604
                     /locus_tag="PKM94_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOD65613.1"
                     /translation="MEPLQIVVIGAIGAALVFDIINGFHDAANSIATVVSTRVLNPRL
                     AVLWAAFFNFTAMFVFVPRVADTVSKIVRIDGKDTVYVYVVLAGLIGAIIWDLITWWL
                     GLPTSSSHALIGGVAGAGLAYRGLDVIRWDNVWKTVQFIPLAPMIGLVLGFSFMLGVF
                     WLFRRWRPNSVDRLFRRGQLLSASLYSLGHGGNDAQKTMGIIMALLIAGGMMSPDTKL
                     SLGNWDTMWIIVSSNLAMGLGTAFGGWRIVKTMGMKITKLKPVGGFCAETAGAATLFV
                     ATHWGIPVSTTHTITGGIVGVGATTNLSGIKWGVVQRVVWAWILTLPAAGLIAAGSFW
                     IMQHLFISP"
     gene            16745..18142
                     /locus_tag="PKM94_02900"
     CDS             16745..18142
                     /locus_tag="PKM94_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOD65614.1"
                     /translation="MTPHIPAPGSRRRRPLYLKILYGLLLGVIGGVAANLAAGGTDPT
                     VAWVIHHVTEPVGQLFLRLLLMVVVPLVFSSLIVGVAGIGDLRTLGRVGLKALAYTFA
                     ISTISVVIGITLSNMIRPGERVDPATATALESRYGTEAAERVEAVQTGSGESPLMRVV
                     RTIVPANPLAAVGGETPNMLHLMFFALALGIAATLVPAGLARPFVSLMESLFGLAAKL
                     IDLIMLLAPYAVACLVFTNLAEFGLDLLGALGWFIVTVLLGLALHMFGVYSLSVYLLS
                     RLNPLYFFRRIRTVMLTAFSTSSSNATLPTALAETQRNLGVPREISTFVLTVGATANQ
                     NGTALYEGVTVLFLAQLSGVDLSLGQQILVAYMAILGGIGTAGVPSGSIPFIVIVLAT
                     IGVNPALIAVILGVDRILDMCRTTLNVTGDITAATFVARSEGYEFAPSGGNGDPAEPS
                     AHGPEAAEEGGSGSR"
     gene            18142..18954
                     /locus_tag="PKM94_02905"
     CDS             18142..18954
                     /locus_tag="PKM94_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOD65615.1"
                     /translation="MTTAFDTAILGATGRPVRRLGLSASYWPGKKTIYEAVDCGVTLF
                     FAYGFDFQMIRALRNLFRTGRDRFVVVTGAYNLIWTHTDVRKSCEKRLRQLGTDYLDY
                     FLFLGVMKPREFPARVQEDLLRLREEGKVRGVGVSCHDRRFAGQLIAEGRVDTLMMRY
                     SAAHRGAETDIFPHVAAHHPTVISYTATRWRQLLRRPRGWPADRPIPTAPQCYRFVLS
                     HPCVDVALTAPSNAAQLRQNLLALEQGPLADEEMAFMREFGDVVHGRRPWFM"
     gene            complement(19049..20344)
                     /locus_tag="PKM94_02910"
     CDS             complement(19049..20344)
                     /locus_tag="PKM94_02910"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOD65616.1"
                     /translation="MAKTLKETLADLIPKWREERTRLAKEHGDVKISDVTIAQAFGGM
                     RGVKGLVCDTSVVEPDQGLIIRGIPIGKLTDRLPEEIFWLLCTGNLPTADELKGLQKE
                     LRAAAEIPPYVLKVLKAMPKTSHPMAMLDTGILVMENESQFRKRYTDGMKKDVYWEPA
                     LEDSIRILGTITGIAAAVYRIKYFKGDLIELNKKEDWAADYVKMLGLPPHKGDTARMM
                     RLYLVLHCDHEGGNVSAFSAHTVGSALSDPYYALSAGLNGLAGPLHGLANQECLAWVL
                     ETIEKFGGAPSEEQLRKYAWETLNSGKVVPGYGHAVLRVTDPRFTAFIEFGKKYLAKD
                     PVFQTVNRVFNVVPDVLKEQGKAKDPWPNVDAASGSVLYPFGLTQFEYYTVLFSVSRA
                     MGILSQLVLNRALGTAITRPKSVSTEWLGKQVKKASVPA"
     gene            complement(20501..21865)
                     /locus_tag="PKM94_02915"
     CDS             complement(20501..21865)
                     /locus_tag="PKM94_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOD65617.1"
                     /translation="MRASDFTALRSLLSSPEALVLEVEAHDAYHHDATDFRGRPAAIV
                     FAAGEADVVAAVRFCAERGLPLVGRGAGTGLSGGCVPANGELVVSTERLTELSIDPAR
                     RIAFCGPGVITKHLQDQALVHGLAYPPDPASFDQSTLGGNVAENAGGLRCKRFGVTRD
                     YVLGVRGVTGEGRLLETGIFNQNRGFDLMDILIGSEGTLAIITRIALRLILAGATGIT
                     ILVAFRQPEDAARAVAEITGSGLIPTVLEYVDGDAAAAANAYRRTEGIDRAAAVLLIE
                     TTERDPEEETAHIRAVCERHRASYLRVEPDAQAAETLWDVRRNISTALKAMAAIRVSE
                     DVAVPNSKFPSLVAFVMEMNAASPLRINSYGHAGDGNLHVNFISMTGSEADRAEIGRR
                     VEQLMRRTIALGGTLSGEHGIGLAKRRFLSLEFDPATRAAMQKFKDVFDPHDILGRGK
                     IFEK"
     gene            complement(21948..22193)
                     /locus_tag="PKM94_02920"
     CDS             complement(21948..22193)
                     /locus_tag="PKM94_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD65618.1"
                     /translation="MPTYQYVCNECGCRFEEFQRISELPLTECPKCQGYVERVISGGA
                     GFLFKGSGFYITDYRKKDYKDAAQKETSGITTKTENS"
     gene            complement(22302..23525)
                     /locus_tag="PKM94_02925"
     CDS             complement(22302..23525)
                     /locus_tag="PKM94_02925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD65619.1"
                     /translation="MAAMKLSSNMALLRPSGTTAYFSRVRDLVHEGQDIVSLATGELH
                     FATPEHVARAGMAAISEGHTKYTLSSGIRDLRTAIARFVSETHGLRFAPDQVVVASGS
                     KQALFNALYAISGPGGEVIIPSPYYPSYPEQVKMAGAVPVLLPTRPEDGFQIDEPALR
                     AAITDRSRAVIVNSPHNPTGAVCSESALRAVAAVARERGLWVITDDIYEAIVYPPAES
                     YRLLRLAPDLADQVIIVSGFSKSYAMTGWRIGYAVGPRKVIEAMTLVQSHVTNNASSI
                     SQHAALAALEEPDFFRKDLVIRLQTQRDRALAALRAIPGITCPNADGAFYLFPDISGL
                     LGGKVGDRPMMTSADLALFLLERHGVAVVPGSAFWAENHLRISYAAAEHRLVEGLRRL
                     GEGVAQLRPRVRNGN"
     gene            complement(23630..25300)
                     /gene="pckA"
                     /locus_tag="PKM94_02930"
     CDS             complement(23630..25300)
                     /gene="pckA"
                     /locus_tag="PKM94_02930"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOD65620.1"
                     /translation="MTQYIIDIKTPAQKEAGAVKSEYGLDNHGFTNLHNVYWNLPSEA
                     LYEEAIFRGEGKVTHGGALTVSTGKHTARSANDKFVVREPSTEDRIWWGEYNRPFSLD
                     KFHLVWNRLQGFLQGRDLFVQDCYCGADKNYRLPIRVITEYAWHSLFARNMFIEPATN
                     DELRRFVPEFTIVSIPSFKGLPQIDGTLSETFILLNFDEKLCLIGNSGYGGEIKKSAF
                     TVMNFLLPLEGVLSMHCSANVGDDGASALFFGLSGTGKTTLSADPKRGLIGDDEHGWS
                     DEGIFNFEGGCYAKVIALSPTAEPQIYACTRKFGTILENVAFDTVSRLLDLDDERLTE
                     NTRAAYPLEYIDNAVPDKQAGHPKNIVMLTCDASGVMPPIARLTPEQAMYHFISGYTS
                     KVAGTEVDLGKEPEITFSACFGAPFMVHHPAFYADMLRNKMLKYGATCWLVNTGWTGG
                     PYGIGKRISIHHTRTLLNAALDGTLSQVEYRTDPVFGFQVPKSCPGVPDKVLRPIEGW
                     EDPKAYEDKYLQLAALFIENFKKFADGTPPEVVKAGPTKEGISALRGA"
     gene            complement(25297..26514)
                     /locus_tag="PKM94_02935"
     CDS             complement(25297..26514)
                     /locus_tag="PKM94_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD65621.1"
                     /translation="MSISQLAREVKESPTLKLNEAARLLKERGEPVIHLGAGEPKSKV
                     PIDAVLASAAKLTSAEIRYTPTEGIPSLIKAIIRYTEENYNKVVGPRNVIAFSGAKQA
                     FYNLMMTIVNPQDEVIIVAPYWVSYPEIVRMVYGVPVIVTPESGRFEPTMADITQQVS
                     SYTKAIVINSPNNPSGAVYSEDFIGEIVQYCEKKGIYLIMDDIYHKLVFSDGKWTPCY
                     KYAKDQSENSRLIVVNGVSKSYAMTGFRIGWTVANQNITAAMINISAQNTSCPSVVAQ
                     AAAAGALMGVQSSVESLRLTLENNAKVMVNELRAFNGVVIDPPKGTFYCLPDFRAYQK
                     DSVKLSKFLLDVARVVSVPGKEFGLEGRLRLSFCGTIKEIKEGVARIKWALDPESPNE
                     IYIGDRKMRRDWK"
     gene            complement(26519..27511)
                     /gene="pta"
                     /locus_tag="PKM94_02940"
     CDS             complement(26519..27511)
                     /gene="pta"
                     /locus_tag="PKM94_02940"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOD65622.1"
                     /translation="MDAVSLIKERARKKHGTVVLPEGAEPRSIEAAKKLTAEKIARVI
                     LLGDPDKIAAEAKGRGLQLGAVEVITPRRHPEFEAYAHELYEMRKAKGLTPDQAREQM
                     ATELYFGAMLVRHDRADASVAGAVNTTGNVLRAAIHVLGLKPGITTVSSCFLMTIPEY
                     RGEKNKVFVFADCAVVPNPTPEQLASIAVSSADTLRFLLGEEPRVALLSFSTKGSAAH
                     EDVDKVLAALALLKKEHPDLKADGEFQLDAAIVPEVGAKKAPGSAIAGSANVLVFPDL
                     DAGNIGYKLTQRLAKATATGPIIQGLAKPANDLSRGCSADDIVDVSAIATLLRG"
     gene            complement(27532..28672)
                     /gene="prfB"
                     /locus_tag="PKM94_02945"
     CDS             complement(join(27532..28623,28625..28672))
                     /gene="prfB"
                     /locus_tag="PKM94_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOD65623.1"
                     /translation="MLAELKIRVPELKGRLKSLRGIFDLGARRDKVKELEDASGAPDF
                     WDDNQRAQGILKELAVHRRWLESYERLAADLTAVEELLELADAVESPGELVELEATLD
                     GVEKELARIESAALLSGPDDHRNAILSIHPGAGGTESQDWADMLFRMYNRWAERNGFT
                     VELMDYQPGDEAGLKSATLEIKGDYAFGYLKAESGVHRLVRISPFDAAARRHTSFVSV
                     HVFPEVEGNIEIEIREEEVRIDTYRSSGAGGQHVNKTSSAIRLTHLPTGIVVTCQSER
                     SQHKNKEAAFTVLKSRLYQLKREEEAKKMAKFEESKKKIEWGSQIRSYVFQPYQMVKD
                     HRTSVETGNVQAVMDGDLEEFIQAFLTDPELNDSLGALAPTGNTQ"
     gene            28839..29477
                     /locus_tag="PKM94_02950"
     CDS             28839..29477
                     /locus_tag="PKM94_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD65624.1"
                     /translation="MQVQTLVVGAFQVNCYLYWGDAAGRGVIIDPGDEEERIADAVTK
                     AGFEPAAVLLTHGHGDHIAAVSPLMKQYGIPLWAGAGEEKLLANPTANISALVGRPVT
                     TPVPDKLLRDNEVVSAGPFDLRVLATPGHSPGGVCYLDERAGVLFTGDTLFWGSIGRT
                     DLPGGSHTQLIRSIRAKILTLPDGVICYPGHGPTTTVGGERVNNPFLMDDYV"
     gene            29470..30315
                     /locus_tag="PKM94_02955"
     CDS             29470..30315
                     /locus_tag="PKM94_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOD65625.1"
                     /translation="MSEPNIARLATPHLTDFHVHCDYSTDAVGTIDEYCRAALARGLA
                     EICFVTHYDTNPAVDSGDCFVRIKGVERRAAPEHLAPYVDEVHRAHDEYYPRGLMVKL
                     GIEVGWWEGCEDTVRGAIERYPFDYVLCGIHDVGDVCICSHDTEGNLGRMAPERLIEE
                     YFRQAVLAARTGLFSALAHLTYYRRFGEQYYGSIINELHRPYLPELWEALRAGGTAME
                     INTSAIRHGLGEYYPPPGLIHAARRAGVRVERLGSDSHRPEQVGLDFDAAAPLVAEQA
                     PYYGE"
     gene            30324..30920
                     /locus_tag="PKM94_02960"
     CDS             30324..30920
                     /locus_tag="PKM94_02960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOD65626.1"
                     /translation="MAATRSPKKLPRSFYARPTLEVAPDLLGKFIVYRSPRGRLAARI
                     TEVEAYIGQDDPACHASRGKTARNEVMFGPPGFSYIYFIYGMYYCLNVVTEPEGQPAA
                     VLVRAADPAEGLDLIARRSSGNGPHAWLAGPGKLCRGFGLTRRQNGIDLTRDVLYLED
                     RHAPVEAVGRSPRIGIRVGAERMWRFYDQNAKSLSRPG"
     gene            30946..32298
                     /locus_tag="PKM94_02965"
     CDS             30946..32298
                     /locus_tag="PKM94_02965"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOD65627.1"
                     /translation="MAKSSPVKASETSTKNSGKPKRREHPRSVVSVRDRTIPISVFGN
                     VMKQFDMAAAKLNLEPELLNFIKLPRRSTIMHLPVQMDDGTWRMFLAYRVQHSIARGP
                     AKGGIRYHPDVTLDEVQALASWMTWKSAVVNIPFGGGKGGIVCDPTKLSPSELERLTR
                     RYAADMVDLFGPDQDVPAPDVGTGPQVMAWFMDTYSMRAAHPVPGVVTGKPLQLGGSR
                     GRVEATGRGVALCVREAARYLGFELGRCTAAVQGFGNVGSISAKLLHELGVKVTHVSD
                     VNGALRDPHGLDIEALLEYAQQKRTIVGFPRAEAIEAADVLAAEVDILVPAALENQIT
                     HANAKTVRARIIAEGANGPTTPEADAILNKKGIMVIPDILANAGGVTVSYFEWVQNRI
                     GYFWSEEEVNRRLEEKMVMAFNDVLRVAQEHHVDMRIAAYMLAITRVVEVVRLRGIYA
                     "
     gene            32432..33049
                     /locus_tag="PKM94_02970"
     CDS             32432..33049
                     /locus_tag="PKM94_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD65628.1"
                     /translation="MDADFLQRALLAGPAILFSLTFHEFSHAYLAYWFGDTTARDLGR
                     LTFNPLAHLDLFGTIVMVFSQFTFGWAKPVPVNPWNLRNPRRADFWISAAGPLSNLTL
                     ALAFGSLFRILGPHAGSLPEAVPLFLMFAVQVNIALAVFNLIPLFPLDGSHMLRNVLP
                     EEMGSALDSFDRIAPFVLLLLVVTGAVSVILVPIMRVLIPLLLGA"
     gene            33051..33737
                     /locus_tag="PKM94_02975"
     CDS             33051..33737
                     /locus_tag="PKM94_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65629.1"
                     /translation="MRTRRGIVIMPSAALALVLSAGCAGGYRGGAPETRADLDRVEVT
                     ADAYLFDARLVREGKPTSVRLEIYYTDTLIGLGGRGYLGKGALKGWLREDSLRVYFPG
                     SNEYVYEAVPTLLESDACAQAEVRVPLAGLLSTTPDSLGAAEDLEVEADYVDPDEPRF
                     VIRGRGCPWRLEIRYDRRDDRWQVKEFRFADGADLWLTAQRRTVKHGAKMSAGRFAVA
                     IPENAVRIVP"
     gene            33812..35728
                     /locus_tag="PKM94_02980"
     CDS             33812..35728
                     /locus_tag="PKM94_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65630.1"
                     /translation="MNLYRRALAALKPYWKHLVTASVSAALSAVLAGLLVWMLGPLLM
                     VLFEVSDPMATVAAPTATAEVRPATPGEATGNSGMIQKLSSRLGKVKESMQAWVDGIV
                     RSDNRRQTLLNFCWLAFLVVIAKNVFLYLRGYFMAYVQQALVRRFRDQLFEKYQRLPL
                     GYFHGRRTGQVISRITNDVLVLNDSVDIGFNNLVTDAITVIVLFAFLVILSWKLTLLS
                     MIVMPLVFVFIWFVGKKLRKYSARTQERMADVNSVLEEAVTNLRIVKAFSTEEFETGR
                     FFRATHQYFRSLLRMTRIRHLAAPINDMLATTAGLFILLVAGTRIIAGTGELSAGDFI
                     TFIVAMLAMIKPVKSLSQIHIKLQEGMAAAERIFEVLDAPETVGEPAAPVVLPEFREV
                     IRYEGVWFRYETSDDVLRDVTFEVRKGEVVAIVGPSGAGKSTLLDLLPRFYDPQRGRV
                     AIDGHDIRTLSLASLRRQMGIVTQETLLFNDTIANNIAYGLTDVPRERIEEAARIANA
                     HQFIAEFERGYDTPVGNRGVMLSGGQRQRVAIARALLRDPAILIFDEATSALDTESEL
                     LVQEAIDRLMRDRTVLVIAHRLSTVKNADRIIVLDRGRIVECGSHSDLLRHNGLYNRL
                     YTMQFQDVDAHTPA"
     gene            35709..36842
                     /locus_tag="PKM94_02985"
     CDS             35709..36842
                     /locus_tag="PKM94_02985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD65631.1"
                     /translation="MHILQLDSIEPETYGGMEEWIRLVSGGLAARGHRVTVVGRTGAE
                     FLRRVELDTRGVETLHLAIAGDFGPLVIARLRTFMRQRAVDIVTVNFNKDIRLAGIAA
                     RWAGRPRVIWRVGLDIAGDRLRHRLLTPRLVDAVITPSAALKKQIVAHGYISPEMVTV
                     IPNGIPDRPVHEQRPQAARRLREKYGLPPAALVAVTSGRFVDQKGHAYLVAAAGEIIR
                     QAPDIRFLWLGDGPLETRLKAAIADAGLESFFVFAGMLRDFDLELAGSDFMLHPAVEE
                     PFGFVLIEAMRAGLPVAATRVGGIPEVVAEGSSAVLFPPRDPAALAAAAVALACDSDA
                     RTRLGRAGRERFEQRFSADRLVDDVERFFLSQLEEAGQSHGTA"
     gene            36829..37935
                     /locus_tag="PKM94_02990"
     CDS             36829..37935
                     /locus_tag="PKM94_02990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD65632.1"
                     /translation="MGRLKEFEYSFKRFFYRATASLLRPRPVGEGPLDGAALRKVLFL
                     RPDKLGDMVISFPVFDALRTRYPHLRIGVLASPRNSAITLGDPRFDEIYMYTKRAPHD
                     LRELRRLRRAQYDCVVDLVYGDSVTTLYLSEYVARGKPRIGVGKERYARYYDFNRSGR
                     GQILECTLQVLAAFGIDPAAVNPYAGPHLSRSDQELAAAFLGAAAADGAGSLTVGLNL
                     SAGMPSRLWQPEKSAELAARILAARPRARIIVIVVPGDRRRGQELVQAVRDARVHLVP
                     DGLTLRQVAAVISRLDLLVTPDTSLVHIARALQVPVVGLYSCHERNFRQWRPYGQDIG
                     AVVAPSEDNIFAITVDQVMAAFEQVAAGRLGVEE"
     gene            37935..38717
                     /locus_tag="PKM94_02995"
     CDS             37935..38717
                     /locus_tag="PKM94_02995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD65633.1"
                     /translation="MGLTVVVITKDEEANIERCLESVAWADELIVVDSQSTDRTAELA
                     RRRGAVVCDVEWRGFGPAKRRGVALATQEWVLSLDADEVVGTELAHQIRQVAAGGGEH
                     DGYYVGRRTNFLGRWISHCGWYPDPVLRLFRREKGNVNEAVVHEQVEVDGSVGRLSGE
                     LLHFSYPTLEHYLAKSAVYTTLGAREAFRRGRRARWYDLVIRPPISFFSHYIVRQGFR
                     DGMEGWIISVMSAVAVFVKYAKLRHLQRTATPEQREQYGFGD"
     gene            38701..39744
                     /locus_tag="PKM94_03000"
     CDS             38701..39744
                     /locus_tag="PKM94_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD65634.1"
                     /translation="MALEIDLQPGDKILISRTDRLGDLILALPFVETIKRRYPECRVD
                     VLASLYASPILENNPHIDRIVRVQNDQLWTDNLYKKDLLHRIRMEGYRAVVALYPERQ
                     ISQLFQRAEIPIRIGTARRFHSIYFTHHVTHSRKANVKHEYEYNLDFLQFFRDGETVA
                     SPKVYLRERELRNARRILDDVGAEESFVVIHPGSRGSAERWPPERFIELYCQLSREGL
                     SVVLSGSEEEGQGLSDLTAGLPVAVRDISGQTDLRTLAAVLSQARVVVANSTGPLHLA
                     TAVGTHVVGLYPSRKAVSPRRWGPLGEQHRVIQPTGVKCECPPKGCRCMDTISVERVV
                     EEVVALYHVQSVG"
     gene            39752..41908
                     /locus_tag="PKM94_03005"
     CDS             39752..41908
                     /locus_tag="PKM94_03005"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOD65635.1"
                     /translation="MNLAEFIIRIESFNANVNIPLIRKAYEFSDRAHAGQKRQSGEPF
                     IEHCLEVAFILAEQHMDSTTIAAGLVHDVVEDTKIGIDHLRAEFGDEIADLVDGVTKL
                     GSVEFTSREEQQVEYFRKMLLTMARDIRVILIKLADRLHNMRTLEALPPDKQRRIAQE
                     THDVYAPLAHRFGINRIKTELEDLSLKYLESEVYFEIAARLKEKREEREAYIEQVVRP
                     LKEELTKNSIQATVYGRAKHIDSIYRKMRIRNVPMEDMHDLFAIRCIVNTERECYHTL
                     GIVHAMWKPVSGRFDDYIANPKPNGYRSLHTAVFGPLNKIVEIQIRTHQMHYVVENGI
                     AAHWLYKEGRQEMSRSDRQMVWLRDVLEWQKDMTNPSDFLEYLKIDLYSEDIFVFTPA
                     GKLIHLPKGSTPLDFAFQIHSEVGIHCAGAKINGRLQPLSTELQTGDSVEIITNPNRT
                     PSHDWLKLVKTSQARSRIRRWLKQAGFERSVALGKEIVERKLKEERLKMPDGDTLLGY
                     AQQLDKKTIEELLAAIGSGAMGVGKLMTLIEPALEPEETGFVGRVIERIRGSKGIKVH
                     GLDDMMFRFAGCCQPIPGEDIVGFITRGRGVSIHRADCTVAISLQEQAPERKIDVSWD
                     TAKGQSFVVRLEMVVEDRKNMLRDITQAIATADTNVRAAEMYARDTTAVGEFVVEVSS
                     LAHLNRILDKVRKVKGVIKVVRAKGKDSLENVENME"
     gene            complement(41953..42114)
                     /locus_tag="PKM94_03010"
     CDS             complement(41953..42114)
                     /locus_tag="PKM94_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65636.1"
                     /translation="MIDYLFRGGPAPIPILRGDLNGDCGVNVADVTLLIAVLFRGVPL
                     PTELCEEVA"
     gene            complement(42135..42986)
                     /locus_tag="PKM94_03015"
     CDS             complement(42135..42986)
                     /locus_tag="PKM94_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65637.1"
                     /translation="MFRLLQQLLVDDFGEQDLAPYMGVIWDDLDGSKSTSVYNRFEYD
                     GVPGFYIASRANFLDNWDEWDGHVIAHEFAHHLMFSYMERIPGGGDPYDYLFPLYGSE
                     NARAALTEGRAFYAGCSYLGEVEFVNSTFTDPACDVKNLEYPLPNPPYYDDNQNLEPL
                     PPPPLNPQYDGAQVPGAVAVSLIDLDDNADDGNQYIGGALWGHNQDYNGGSWWAGPAA
                     IWHVLTNYDPARQTQTMITAGPFESSERAGSTWAIPSALSLRTCLPLTQLTQYSYRPA
                     TSMATAR"
     gene            complement(43029..43364)
                     /locus_tag="PKM94_03020"
     CDS             complement(43029..43364)
                     /locus_tag="PKM94_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65638.1"
                     /translation="MVLNEGTANYVNKFGIEWPICNVGLGIYESNDGVRYTLDRQLDI
                     GASGYFSFQTNNRRVRFYFVSINASAEIVYADNHDFSLDDETFWLHAHGIQVDNPEYT
                     NVYVPEVYT"
     gene            complement(43355..44290)
                     /locus_tag="PKM94_03025"
     CDS             complement(43355..44290)
                     /locus_tag="PKM94_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65639.1"
                     /translation="MLIMGSVALVSVAVADTTHANIALEYGDLKGSGGVEIVGEPKLG
                     EPFDVVFRFTPVDSFSHSKGIPDTVLIRGNPGVEFVGGDTLWIGSLTRCQTVELKARF
                     VIRAEIKRVVTGWVHAAQTLGQIIPPQFGPNAGVDVGRECVAQFRSKPIDFRQAEEND
                     QISRLWRLKVTDTGLILADTAASNAPSELKRHPVIDSLDQTNRRGTRRPRQLHGDGPP
                     ILRPRQTTPINLSSESPDTIRIAYDPTREVEFFTDSAFTGSVRVFLLEGNAVYTKSGA
                     RTGVFELKGDSGRALFRVEAGSLERILLVHDAAWY"
     gene            complement(44521..46236)
                     /locus_tag="PKM94_03030"
     CDS             complement(44521..46236)
                     /locus_tag="PKM94_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD65640.1"
                     /translation="MKSMLIAALLLLAPAAPAVRAAYLITADSLRAHEMVLAHDSLEG
                     RRVGEEGEMKAARYIESAFAAAGLQPRGTDGSWLQPFEFVRAIELGPGNRLVLNGAPL
                     ALHGEFEPMKQSASLAFDFDTIVDVGYGITVEEADGNYDDYAGKDVAGKAVLIKRYAP
                     SADENPHVDFEPYSSLTSKINTALEHDAAGIFFITPEDQDDTLQPIGPVRITPKDIPI
                     IFLRRAGFEHLGLSLSDPAVLSAEGAVELVKVHDTGYNVLGYLPGETDTTVIIGAHYD
                     HLGSGGPGSGSRYLGNDPQIHNGADDNGSGTSVLLEIARHYAAQAEKPHYSLLFAAFS
                     GEEFGILGSSHFARDMTVDSAKVRMMVNMDMVGRLAEQDNGLAVMGTGTTEAFTVYFD
                     SLRREDVKITQKKSGQGPSDHTAFYNRGIPVLMFFTGAHNDYHKPEDDWDRIDYAGLV
                     TVADVVTDLVSYFDRYPGPLTFQKTKDPDEGKRASTFSVTLGIMPDYVAEVKGLRVDN
                     VQPDRPGERAGLLAGDIITRMGEIEIGDIYDYMNALGRFRKGDTTVVRVNRNAETVDL
                     TVVFE"
     gene            46461..47159
                     /locus_tag="PKM94_03035"
     CDS             46461..47159
                     /locus_tag="PKM94_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65641.1"
                     /translation="MTDSICAACGKPLTAGDRFCAACGAPTDLDVKPVRGGKPASKKP
                     PAGAPRSASTGMRDGAIIVGALALITVGWFVFRTPAVPPQPPSAATPAGHPEVPGAPM
                     GGMTGGLSDLPADYDGLVQAGNRYMDAGNYAVAAESYRRALALDPSSPDVRTDFGACL
                     HGMGLPERAIEEFREVMQTHPEHGIANFNIGIVYLDMGKPDSARFYWEKYLQLEPTGA
                     AAAKARELLGNLNG"
     gene            47152..48279
                     /locus_tag="PKM94_03040"
     CDS             47152..48279
                     /locus_tag="PKM94_03040"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HOD65642.1"
                     /translation="MDSAATFLGNWAEATWAMVVDSALLLLLSFVLAGLLHLVLNART
                     IGKLVSGGRRREVFATALVGVPLPLCSCSVLPVAYQLRRAGVSKAGTTAFLIATPESG
                     VDSVLLTWSLMDPLMTVARPVAAFLTAFTAGNIEAAFPDDRPTGKDGAAAGGDGCACG
                     CAAEPNAAVAPVRIADRAPRASWLSRVLAGIRYGFTDLFSDLAVYLLVGYLLAGLVAA
                     VFGSALLSLPPWLTAGWGGYAGAIVIGLPLYICATSSTPLAAALLGAGFSPGAVLVFL
                     LVGPATNVASMTVVGKILKGWGLARYLGSIVIVAVLCGIALDWAYRTVGIAAAFRAGG
                     HDHASWYHVAAGLLFVLLLARVLAMKIRRRLRRGRMTPAAG"
     gene            48348..49259
                     /locus_tag="PKM94_03045"
     CDS             48348..49259
                     /locus_tag="PKM94_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD65643.1"
                     /translation="MADQTFPCVSLIIPTYGREEPLVACLRCALNQSYRPLEILVIDQ
                     TGEHDPETVRFLQGAADRVRVIRHAPPSAVTARNCGLHEARGEILIFIDDDTTFGSGF
                     AAAHVAAHRRGAEVVQGRVVEPGRGVAHRPQWVRPWLRFVGSNTHDRAGVTNTLTGCN
                     FSLSRRAADRVGEFDPLFCGALVREDADYGARCRRAGLRMVFDADACLTHHRAAGGVD
                     RGVTGREREFDLSLLRNELYFARKHFAPPAVWLYKLRMARRLRRIARRSGGVPPHPIR
                     KLIRQADGQARLMVREGELPELSDAPR"
     gene            49313..>49660
                     /locus_tag="PKM94_03050"
     CDS             49313..>49660
                     /locus_tag="PKM94_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HOD65644.1"
                     /translation="MRWSALIENPVTRSVLDFLFPPLCLGCGVFYDGDNLVCGRCEAG
                     LAALAFEGTICAGCGAVMSASGRCPECGPAFWPLFAFGDYRPPLKEIILQFKFKGIVA
                     PADWIAARLAPVVD"
BASE COUNT         8601 a        15459 c        16650 g         8950 t
ORIGIN      
        1 ggaccgggtg caggcggtca tcgagcgggc acgcacggaa agccgcacga caatcgtggg
       61 tgcggaggcg atcgagattt tgtgcgccta cggcatcccg gcggccgagt accgcttcgc
      121 cagcagcgag cgagaggcgg ccgcgattgc gaaagggatg gggctgccgg tggtgatgaa
      181 ggtggcggcg ccggcgattc tccacaagac tgaggtcggc ggcgtgatgc tcgacctgcg
      241 caccgaacga gaggtgcgcg acgcgttccg cgaactccgc aaacgggtcg cggccgagcg
      301 cgccgcgggc gagacgttcg cggtggccct ccagcaaatg gtggcgggcg gggtcgagac
      361 ggttatgggc atgaccaccg atccctcgtt cgggcccctc atcatgttcg gactgggcgg
      421 gatctacgtg gaaatcatga aagacgtcgc cttccggatc aacccgctca cgaccgcaga
      481 tgtgcgcgac atgatgcgct cgctgaagtc ctacccgctc ctgaaagggt accgtggggc
      541 cccgccggtc gatctggcga cggtcgagga cacgctgctg aaggtgtcgc agctggtgaa
      601 ggatttcgac gccttcagcg aagtcgacat caaccccttc atcgcctcgc cggagcgcgc
      661 gcggtgcaag gcggtggatg cgcggttcat catgaaagag ggctgagggc cggattatcg
      721 cttgaatttc cgcaccggcg gcgtatcttg gcaccacccc cggcggcgac ccgccgtccg
      781 gggcggagaa gggtgggaac acccgggagg acgcgatgaa actggagctg aaagcggtct
      841 cggtctggac ggcggtcaag atcggttttt tcctgaacct gatcatggga ttcatcatgg
      901 gcctggtggc ggccctcttt atggccccgc tcatggcggt gatgtcgacg atgatgccgg
      961 cgggagccgg cggctcggag ttcgcggcca tgccggtggg actcctcctg gtgattctgc
     1021 cgatcgggta cgcgattttc ggggcggtgt tcgggaccat caccttcgcc atcggcgcgg
     1081 gcgtgtacaa tgtcaccgcc cgcattctgg gcgggctgga aatcacgtac acggtggtgg
     1141 agtgggggga tccgagcggc gcggcgcaag cgccgccgcc gttcccgcgc tatcccgcac
     1201 ccgcgccgcc ctatccggcc tatccgccgc ccaccgggcg gccgggaccc tactccggct
     1261 cgccgatgcc gcccccgccg ccgcctccgg aggcgcgccc ggagccccag gcgccgcacc
     1321 ggccgctccc tgaggcgccc ccgccgggcg gacctcccga ggaacccgag aaaccgaccc
     1381 agccgtagat gccggaaatg accgatcgat ccattcgcgt acgcatcgcc ccgtcgccga
     1441 cgggctacct ccacgtgggg acggcccgga cggccatttt caactggctg tttgctcggc
     1501 actccggcgg gaagttcctc gtgcgtatcg aggacaccga ccaggcgcgg tcgcaggcgg
     1561 atctgatcgc gcccattctc gacgccatgc gctggctggg actgcaatgg gacgaggcga
     1621 tcacgtacca gtcgcagcgg ctcgacacct acaacgcctg cgcgcggcgc attctcgagc
     1681 tgggccgcgg gtaccgctgt ttctgcacgc ccgcggaact cgagcgggga cgggaggagg
     1741 ccaaggcgcg caagcagccg ctccgctacg accggcgctg cctgcggctg ccgccggacg
     1801 agatcgcgcg gcgcgtggcg gcgggcatgc cctacgcgat ccgcattctc gtgccggagg
     1861 gggagacgcg tttccacgac ctcgtgctcg gcgaccttgg ccggcacagc gacgatatcg
     1921 aggacatggt gatcgcgcgc tcggatgggt cggccaccta caatctcgcg gtggtggtcg
     1981 acgatcacga aatggggatc acccatgtga tccggggaaa cgaccacgtc acgaacacct
     2041 tcaagcaaat ccacgtgtac cgggcgctgg ggtgggagat tccgcagttc ggacacctgc
     2101 cgctgattct ccgcccggac aagaagaaag tctcgaagcg gctgggggac aaggacgtca
     2161 accagtacag ccgtgaggga atcctgccgg aggcgatgtt caactacctg tgcctgctcg
     2221 ggtggaatcc caagaccgac cgcgagatct atacccggga ggagctgatc gcgctgttca
     2281 cgccggccta cttcaacgcc tcgaacgccg tcttcgacga ggaaaagctc gaggccttca
     2341 accgcgaaca cctcctccgc cggagcaatc acgacctggc cacgatggcg gccccgctgc
     2401 tggtcgaggc cgggctcacc accaagtatt ggctcgagac gcgctggaaa tatctctgcg
     2461 aggtggtcgg gctgctcaag gaccgggtgc ggcgggtgac cgatttcgcg gccctcggcg
     2521 ggtacttctt cgcgttcgac taccgctacg acgcggcggc ggcggcgaag cattttcacg
     2581 ggggggcggc cgacctgctg gcccgcctag ccgaccagct cgaaccgctc gcggagctga
     2641 cggccgacaa ctgcgagcgg gcggtggagg cggcggccgc tgcggcgggg gtgaagaagg
     2701 cgagcgtcat acacccgacg cggctggcga tttcggggat gccggccggg ccggggctgt
     2761 acgagatcat ggcgctcctg ggtcgctcgg cggttctcga gcgcctgcgc aaggccattg
     2821 attatattca cgcaacgccc ggggcctcat gagaggcccg gaaaggggcc atatggcgaa
     2881 gaccaaagag tacatcgccg agacgctcaa gaaggcgatc aagggagaag aggacggcta
     2941 cacgttctac gatctcctca tggagcgggc gaccaacccg gacgccaagc ggaaactgaa
     3001 gaacctccgg gacgacgagg tgcgccacaa gcacacgctc gtggagattt tccacaagca
     3061 catcggaccg gagatcggcg acctgccgcc gaaggggatc aacgccctgg cccaggtgtt
     3121 ccgcaaggga cggctggagg aacgcaagag cgagatggaa ttcatcaatc tcgccatcga
     3181 ggcggagctg gcggcgaccc ggtactacca gacggagcgg gacttggtgg acgatccgga
     3241 atttcgcgag gttttcgaca gcctggccga ggaggagcac cggcactacg aactgctcca
     3301 ggcggaaaag gacgcgattt ccgggaacta cttctggttc ggtctcgacg atacgtcgcc
     3361 gctggaacac tgacggcgcg gcgccgggac cacgccgccg ggtgaggcgg cggtgacggt
     3421 gaggaacgta tgcagtggga gtgtgtcatc tgcgggctta tccacgagga cgatgagccg
     3481 ccgcccgtgt gccccgtctg cggcgccccg ggcagccggt tcaccgaacg gtacgaaccg
     3541 gaagatgagc ttgatgaagc ccgagaggag gacgaggggg acgatgatga cgagtactac
     3601 ggcgaatttc agtgaccgca ggacggcgaa cgatggcggc gcctgagcgc gtaaagaccc
     3661 ggaccagaac cctggaggtc ggtgacgagg cccccgattt cacgctgccc acccacaacg
     3721 agggcgacct caacctggcg tggtacgagg ggcggcggat cgtggtgctg gccttctacc
     3781 cgggggactg gacgcccgtg tgcgcgaccc agatcccgga gtaccagggg ttgatcgacg
     3841 agttcgcgcg gtacgaggcg cagttgctgg cgatctcggt ggactcccgc ccctgccacc
     3901 tggcctgggc gcgctccctc ggcgggctgt cgtttcccct gatgtccgat ttctggccgc
     3961 acggcggcgt ggccgaaaag tacggcgtgc tctccgaacg gggcttcgcc gagcgcgcgg
     4021 tagtcgtgat cgacaagcgc ggcatcatcc gctacatcga ggtggtccct tcatcggaac
     4081 tgcccgacaa cggcaagctc tttcggtgtc tcgcggagtt gaagcgcgaa gacgagagcg
     4141 ccgcggcggc cggcgaccgg ggcgcggggg aggaccgctg aagagcgcgt tgatcccggt
     4201 gggcggtgcc gcgccgccgt tcgacctgcc cgcctcgagt ggcggccggc tgggaaccga
     4261 ggccctggcc ggacggtttg cggtaatagt gttttacccg aagaacaaca cgcccgggtg
     4321 aaaccaccaa ttggccggtc tggaagaccg gcgggcggat ttcgagaggc acggagcgcg
     4381 ggtcctggcg gtcaatccgg cgaccctcgc ggaacaccgc cagtgggcgc tcctgctggg
     4441 actgcacttc ccgatcctgt cggatgccga ctggcgggtg acgcgggcgt tcggctgcaa
     4501 gacgcccttt ctcctggtcg tgcggcggac ggtgtatgcg ctcgggcggg acggacgggt
     4561 ggtcttcgcc gcgcgggggc acgccgcctg ggacgaagtg gaggcggcga tccgggcggc
     4621 ggagtaaggg acaaggccgg caagaggata tgagcgaaca cgaagcaaga cacgactcgc
     4681 gcgacatcta cgatgtgtcg tttatcgggg ccgggccggt ggccatgtac gggatgtact
     4741 acgccgggct gcgcatgatg cgggccaagg ctatcgacat gctcgaagaa gtggggggcg
     4801 gcctcatggc gctctacccc gagaaataca tctacgacgt ggccgggttc ccgaaagtgc
     4861 tggccaagga tctcgtgcgg cagttcgagg cccaggcggc gcagtacccg accaacactt
     4921 tctgtctcgg agagaaagtc accgaggtga cgcgcgaggg ggacggcgtg ttccggctca
     4981 ccaccgaccg gcaggcgcac tacacgcgca ccgtagtcgt gtgcgccggg ctcggagcgt
     5041 acatccccaa acggatggac gtgccgaacg tggaaaaact cgagggccgc ggcgtctatt
     5101 acttctgccg gcggctgcgc gattttgcgg acaaggacgt gctcattgtc ggcggtggcg
     5161 acgcggcgtt cgactactcc atgatgctcg gaccggtggc ccggtcggtc actcacatcc
     5221 accgcaactc gttcttctcc gcccacgagg attcggtgcg caaggtgaaa aactcccggg
     5281 tccggctgct ctacccgttc tgggagctga aagagctgcg cggggaggac tgggtggagg
     5341 gggcgaccat tgtgcagacc cggaccggcc acgaggagcg cttggcggtc gacgcggtcg
     5401 tgctcaacat cggtttcctc accaacctcg gaccgatcga ggggtggggg ctggaactgg
     5461 acaaaaacgc cattcgggtg gacacccgaa tgcgtaccaa tgtagaaggt atttacgcgg
     5521 ccggagatat cgtgacgtac gaggggaagc tgaagctgat ctcgaccggc tgcggcgagg
     5581 tcgcgatcgc ggtcaacaac gcgaaagcgt acctcgaccc gcgggccaaa gtcagcccgg
     5641 ggcattcgac ggagaaacat gaggtggtga tgcgcaagct gcagcagcgg cagcagcaga
     5701 gccgcgggga agaggatcgg gagtagggtc atgtatttcg cccttcccaa agagtaccgc
     5761 gaggcgacgg tcgacgatct gcaggcgcgg gcgcaggcgg tccgcgaccg gctcggcaaa
     5821 cgcctcttga tcctcacgca ccactaccag cgtgtggagg tggcggcttt ccacgaccac
     5881 ctcggcgaca gttacgggct gtcgaagctg gccgccgccc agcgcgaggc ggagttcatc
     5941 gtgttctgcg gcgtccactt catggccgag tcggccgatg tcctcaccgc gccggagcag
     6001 aaggtcttcc tcccgaatcc gctggccggg tgtccgatgg cggacatggc cgagatggcg
     6061 gatgtgctgg cggcctggga gtgtctcgag gaattcggcg gcgaccgccg ggtcatgccg
     6121 atctcctaca tgaacacggc ggccgggctc aaggccttta ccgggcgcca cggtgggctg
     6181 atctgcacct cgtccaacgc cagggcggcc ctcacctggg ccttcgggct gcgggagaag
     6241 gtcttctttt tcccggacga acacctcggc tacaacaccg ggatcgccct cggcatcgcc
     6301 cccgaggaga tggtgctgtg ggacttcaag cgggacggcg gcgggctgac ccgcgcgcag
     6361 atggagaagg cgaagatcat cctctggaag gggcactgcc acgtccacac caatttccgc
     6421 gccgaacacg tcgaacaggt gcggcgcgac taccccgggg tgaaggtggt cgtgcatccc
     6481 gagtgccccc acgaggtggt ccggctggcc gacagtgtcg ggtcgacctc gcacattgtc
     6541 gacttctgcc gcaaggcgcc agccgggagc acgatcgcga tcggcactga gatcaacctg
     6601 atccaccgga tggccctgga gcatcccgac aagagaatct tcgagctctc ggggcagacc
     6661 tgcccggtgt gcgccaacat gtaccggacg acgctcaatg acctggtcta cacgctggag
     6721 aatctcgata cggtcaagcc gattgcggta aaagagccga tccgcagcga ggctcgcctg
     6781 gcgctggaaa agatgcttga aatcgcttag catttccgca ggggatcctt gactcttccg
     6841 gtagattttc cgtatattaa cagcttggat tagaaatcat tatatgtttg ccagataagg
     6901 aaatggagcg atggcagttg atgttctcgt cccgcagatg ggggaatcgg tgctcgaggg
     6961 aactatcctc gaatggaagg tcaaaatcgg cgaccgggtg aaagcggatc agccgctggt
     7021 cgagctgatg accgacaaga tcaatatcga aatcccgtcg gagacggacg ggattctcac
     7081 gcacctgtat gccaaggagg gggaggtcgt cccggtggga acgcgcatcg ccaccattga
     7141 cgacgggggc gcggcggcga agacggccaa gacctccgag gaacggcccc aggcggctcc
     7201 ggcggctgtt tccgccccgg ctgcgccgcc gaagccgcgt ccggccgtgg ccgggacggc
     7261 gccggcgggg caggggaaac tctcgcccaa ggtgcgggtg atgatccggg agtactcgct
     7321 ggatccggcg accatcgtgc cgaccggcaa ggacgggatc gtcaaggtcg aagatgtgct
     7381 ccgggcagtc gaggagcgga gcctggccca agggtttgcg gcggcgccga tccctgcgcc
     7441 cgcgccgccg gagagcgccg cggccggccc acgtccggag acgccgccgc cggcggtccc
     7501 tgccgccccg aaggtcacga tcgaggtccc caagttcgcg cccctgcccg aatcgcagcg
     7561 tgaacggcgc gagccggtga cgggaatacg caagtcgatc tccgagcaca tggtgcggtc
     7621 ggtccagacc tcgccgcacg tgaccacgtt cgaggaactc gacctgaccg acctcgcggc
     7681 cttccgcaac gagatcaaga cggatttccg ggcaacctac ggggccaata tcaccttcat
     7741 gccgttcatc gtgaaggcgg tctgtgtggc gctgaaggag tacccgaaac tgaacgcctc
     7801 gatgaccgag aaagagatca tctacaagaa ctactataac atcggcatcg ccgccgcccg
     7861 cgaggaaggc ttgatcgtcc cggtgatcaa ggatgccgac aagaagtcgg tcgtcgagct
     7921 ggcggtggag atcgcccagc tgggggataa ggcgaagcgg aaccggctgg ttccggacga
     7981 cgtgagcggg gggacattct cgatcacgaa cgccggcatg ttcggggcga ctgcctcgac
     8041 gccgatcatc aaccagcccc aggtggcgat cctcggcgtc cacgcgattg tgaagcggcc
     8101 ctgggtggtc aatggcgaga tcgccatccg cgacatttcg tcgttcggcc tgtcgttcga
     8161 ccaccggctg atcgacggcc acacggcggt gcagtttctg caccggatcc acgaactgct
     8221 ggccgatccc cggcggctgg cgctgcacct gcggtaggat gtcggtctac acgccacgtc
     8281 cctacggctg ggtgctggag gtcggccggg tcgagtacga gcgggcgctc gcctggcagc
     8341 gcgggctggt caagatgcgg cgcgaaggga tggcccgcga tacgatcatg ttcgtcgaac
     8401 acccggccgt gtgcacggtc ggccgcgacg ggcacgagga gcactaccgg aacatttcct
     8461 cggcgccggt gtttgttgag cggggcggcg atgtcacctg gcatggtccc ggacaactcg
     8521 tggtctactt catcttcaat ctcgcccgcc gcgggcggga tctccacgcc tttatgaccg
     8581 cgatccagca gggaatcgtc gatgctctcg ccgagtggga gatcgcggcc cgcctcgggg
     8641 aggagtacac cggggtctgg gtgggcccga agaagatagc ctcgatcggg gtggcggtga
     8701 agaagtggat cacgttccac ggcgcggcga tcaacctcaa caccgacctg cgcgagtttg
     8761 cccagatgga gccgtgcggg ctcagcgccg acctgatgac ctcggccgcc gagcaactcg
     8821 gccgaccggt cgacatgcag gccttcgcgc aggcgctcct gcgcggctac gccgagcgct
     8881 tccacaccca gttcgacccg gtggcgctcg aggcgatcgc cgaggatctc gaatcgcagt
     8941 ccggcggcta cacaatctag gtttgcgggc gacggggaca acggaaggaa aggagacctg
     9001 atggagttcg aggggaaagt tgcgtttgtg tcggggggga cgcgggggat cgggctggcg
     9061 gtgtgccgga aactcgccgc gcgcggcgcg catatcgtgc tcacctattt ccgcagccgg
     9121 tcgaatgcgc aggaggcggt ggccgagctg gagaagtttg gggtggaggc gaccgcgatc
     9181 cgcgccaaca tggggaacat ggagcaggtc gccgacgtgc tcaagaccat ccgcgagaaa
     9241 taccgcaagc tcgacatcct cgtgtcgaac gcggccctcg ggacctacac cgacatgatg
     9301 aatatcgacc gcaaggactg ggagatcgcc atgcacacga atgcgcgggc gttcctccag
     9361 ttgatccaga tggggattcc gatgatgccg cgggggagca aggtcgtcgc cctctcctcg
     9421 ctcgggtcga tccgctacat tcccggatac gcggcgatcg gcgtgtcgaa agcggcggtc
     9481 gagaatatgg tgcgctacgc ggccatcgaa atggcctccc gggagattta catcaactgc
     9541 gtctccggcg ggttcatcga caccgacgcg ctcaaagttt tccccaacta cgaggagatg
     9601 aaaaagcagg taaccgcccg cactcctttc gggcgcatcg ggcgcccgga ggaagtcgcg
     9661 gaggcggtgg tgtttctgtg cggggacggg gcgagctgga ttaccggcca gacgttgatt
     9721 gtggacggag ggtacagcct gatgtagccg gtcggcggag ccgggggggc gttccggaca
     9781 ggccgatcgg gcgaaaccgg ccgggccgtg accgtgaaga aggcggtaag tttgaccaga
     9841 catcaaaacc tatattgcgg acctatgtgc tccgcaaaag acattgacaa tattgaagtt
     9901 aggccaattt cagcgatcgg gagtgccgca gacggatccc tgtcccccgg tgtcgcttgc
     9961 gcgatccgtc acaagccccg atcaaaaatg ttgcgttgag gatcgcgatt catatatttc
    10021 cgcttttcaa acgccgtgcc ttcaagtgaa aatacagggc cgtgtcaaca gcgggcaaat
    10081 gtgacctgag tgacgatact agtagaaaaa aggagacgcg aatctatgag tgagaagggt
    10141 cgaagcgccg gtggcggccg gcgattatac ggggcgatcc ggtcggtggc ggcgggtttg
    10201 gcggcggcgc tggccgctcc gctggcgcgg gccagcgaag cggacattgt agtccccgac
    10261 ctcaacaccg gcgatttctt cggcatgacc ggccacaacc tcctgctctg ggggatcgtg
    10321 gtctgcctgc tgggtatggt gttcggctgg tacgcctacc gcgggctgcg caagctccct
    10381 gtgcaccagg cgatgcggga catttcggaa ctgatctacg agacctgcaa gacgtacctc
    10441 cagacccagg ggaaattcat catgctgctc tgggccttca tcggcctcat catcatagtg
    10501 tatttcggct ggctccgacc ggtgggcgcg ggcaatctgc tgatcatcct cctgttctcg
    10561 cttgtgggta tcgcggggag ttacggcgtg gcctggttcg ggatccggat caacaccttt
    10621 gccaactccc gctcggcctt cgccagcctg accgggaaag cctacccgac ctttgcgatt
    10681 ccgctgcggg cgggcatcag cgtggggacc ctgctcatct ccatcgaact catcatcatg
    10741 ctggcgatcc tcctctttgt ccccggtcac ctggccggtt actgcttcat cggtttcgcc
    10801 gtcggcgagt cgctcggggc cgcggcgctg cgtatcgccg gcggcatttt caccaagatc
    10861 gccgacatcg gcagcgatct gatgaagatc gtcttcaaca tcaaggaaga cgacgcccgc
    10921 aatcccggcg tgattgccga ctgcacgggc gacaacgccg gcgattcggt cggcccgacc
    10981 gccgacgggt tcgagaccta cggcgtgacg ggcgttgcgc tcatcacctt catcctcctc
    11041 gccgtcatga accccgccat ccaggtgcag ctacttgtgt ggatcttcgt catgcgcgtc
    11101 atgatgatcg tcaccagcat cgcctcctac tggatcaacg gcgcgctggc gcggggccgg
    11161 tacggcgacc aggcgacctt caatttcgag gcccctctga cttcgctcgt gtggatcacc
    11221 tcgatcgtct ccatcgccat gacctacgtt atctcctacg ccatgctggc ggagctgggc
    11281 ggcggttcgc tctggtggaa gctggcgacg atcatctcgt gcgggaccct ggccggggcg
    11341 gtgattccgg agctggtgaa gatatttacg tcgaccaagt cgcggcacgt gaaggaagtg
    11401 gtgacggcgg cgcgggaagg gggcgcctcg ctgaacatcc tgtccgggtt tgtcgcgggc
    11461 aatttcagcg gctactggat gggcctgtcg atcatcgccc tcatggcgat cgcctggggg
    11521 atgagcacct cgggcctggg cgccatcatg gcggccgcgc cggtcttcgc cttcggcctg
    11581 gtcgcgttcg gcttcctcgg catggggccg gtcaccatcg cggtcgactc ctacggcccg
    11641 gtgaccgaca acgcccagtc cgtgtatgaa ctgtcgacga tcgagacgat cccgaatatc
    11701 gcacaggaca tcaagaggga attcggcttc acgcccgatt tcgagaaagc caagctcacg
    11761 ctcgaggaga acgacggcgc cgggaacacg tttaaggcga ccgccaaacc ggtgctcatc
    11821 ggcacagccg tggtgggggc caccaccctc atcttctcca tcatcctgct tctggcgaca
    11881 gaggcggcgg ggctcgaggg ggtgctgacg ctggagcaga tcaaccaggc gctcatgatg
    11941 gccgggtacc acttctcgct cctgaacgcg cccttcctgc tgggcctgat cgcgggcggg
    12001 gcggtgattt actggttcac cggggcgtcg acccaggccg tgtcgaccgg cgcgtaccgc
    12061 gccgtcgaat tcatcaaggc gaacatccgc ctcgacggca cttcgcagaa ggcctcggtg
    12121 aaagacagca agcgggtggt cgaaatctgc acccagtacg cgcagaaagg catgttcaac
    12181 atcttcatcg tcgtcttctt caccaccctg gctttcgcct gcgtcgactc ctacttcttc
    12241 atcgggtacc tcatctcgat cgcgatcttc gggctctacc aggcgatctt catggccaac
    12301 gccggcggcg cctgggacaa cgcaaagaag atcgtcgaaa ccgacctgcg tgagaagggc
    12361 acgcccctcc acgcggcgac ggtgatcggc gacacggtcg gggatccgtt caaggacacc
    12421 tcctcggtgg ccatgaaccc ggtgatcaag ttcacgaccc tgttcggggt gctggcggtg
    12481 gagctggcga tcgagctgac gcaggggacg cgggtggcgc tggccgcgct gttcttcgcc
    12541 atcgcggccg cctttgtcta ccgctcgttc taccggatgc gcatcacgac gaccggagtt
    12601 caggaaccgg ccaagccgaa ccggaaggcg gcggccggcg aaccggcgga agtccgcgcc
    12661 gcgcgctgag cccggtttcc attgagtatt gacagccggc ccgctttttc gagcgggccg
    12721 gttttcttta tggcgcgagc gggtgacagc cggtgtcgcc gcacacggcc ggcgaattcc
    12781 gggcccgggc ggtgctgccc gcgccgcctt gacagtcgcg ccgcctgccg gtatattgca
    12841 gcccggctgc cgcttgacag cggccgccgt gaaggagtgt tactcatgaa gcgctttgac
    12901 atgcgggtgg atcccctcac gaacgaaatc tactacgccg tgccccacaa gggacacatg
    12961 ctcgtgtcgg atcctctgct gaacaagggg tacgcgttca cccgggccga acgaaacgaa
    13021 ttcgagctcc tcggtctact ggcggatcag gtggggacgc tggaacagca gcgtacgcgc
    13081 aaccacaaca tcatcaagtc catgaacagc gaccttgaca aatacgttga gttgatcgga
    13141 ctgctcgacc gcaacgagac cgtcttctac tcgctgttgg cgcaccacat cgaggagatg
    13201 ctgccgatcg tgtacacgcc gacggtcggc ctgggctgcc tgaaacgctc acacatcatc
    13261 cgccggtggc gcggcatcta cgtctcgccc tcgaatgtcg agtggatgga gcaaatcctg
    13321 cgcaacgtcg ggctgcccaa cgtgtcgctc gtggtggcga ccgacggcga gcggattctc
    13381 ggcctcggcg acctgggggc cgacggcatg ggcatcccga tcggcaaaat cgccctctac
    13441 gtcgcggccg ccggcatcca tcccgcctcg accctgccgg tgatgatcga cgtcggtacc
    13501 aacaacgagc ggctgctgaa cgatccgctc tacaccgggt accgccagcc gcgcctgacg
    13561 ggggacgcct actacgaggt gatcgagcgt ttcgtgcagg gcctgcggcg cgtcttcccg
    13621 cgcgccctcc tgcagtggga ggacttcggc aagcagcacg ccgccacgct gctcgagcgg
    13681 taccacgagc gcatcctcag tttcaacgac gacatccagg ggacgggcgc caccgccgcg
    13741 gccgtgctgc gctcggcctt tcgcttgacc ggacggccgg cggccgagga gcggatcgcc
    13801 attttcggtt tcggccaggc cggctgcggc gtggcgaacg ccctcgtcac gctgctcacg
    13861 ctcgagggcg gcctgtcgac ggccgaggcg agacgccgga tattcgcagt cgatgtcaat
    13921 ggactcctgc tccagggcga cccggcggac gcctatcagg cggagttccg gcagccccgc
    13981 gaggtcgcgg cggactggcc ggcgcccaag gaccgggcgc ccaatctcca ggaggtcatc
    14041 cggcacggac ggatcacgac cctcatcggt ctgtcgggac aaaagggagc tttcaccccg
    14101 gagatcctcg cggcgctggc ggcgaatgct ccgcggccgg tcatcctggc gctgtccaac
    14161 ccgacctcgt gctgcgaaac gaccccggag gcggtggtgg cggcgaccgg aggacggggc
    14221 atcatcgcca cggggagccc cagtcggccg gtgacgctgc cggacgggcg ggagatgacg
    14281 gtgtcgcagt gcaacaacct gtatgtcttc cccggcatgg gtctcggggc gatcgtctgc
    14341 caggcgcaga aggtttcgca ccgcatgttt cacgccgcca cctgcgccat ttcggacatg
    14401 gtcaccgagg agatgcggtc gaccgggtgt ctgctgcccg cactcgaaaa cgcccgcgag
    14461 atctcgttcg cggtggcgct ggcggtggcc cggcaggcgc gcgatgaagg catcggcctg
    14521 gccgcctccg atgaccgcct cgcagccctc atccgcggcg ccatgtggga gccgaagtac
    14581 tacccctacc gggcagacat ggggcattag cgtaaacttc gacacacgaa cctgcaatta
    14641 ctcaccggcc cgcctctccg cgcgggccgg tcctatttcg ggacggggga gcgggatcac
    14701 acttggccgg ccggccccgg cggacacttg accggtcaac ctccttattg gctttcggtt
    14761 attcgcggcc gcgacgcccg ggtcgaccgg ccgagcggcg accgacgggg caatatttcc
    14821 gcttaaatcg cttgcccgat ggttcggatc gtgttaagat tgcccctgat ttgtgaccgg
    14881 ggaatatcct cgaacgacgc cgccggacca tgaccgggcg tctcagacgg gagatgccat
    14941 gttagagaaa ctccttccca gagaaaccag cttctttaag ttcttcgagc agacgagcgg
    15001 gctggcgatc gaagtgtgcg tcgaactgga caacctcgtg aattcgtcca acgacgttgc
    15061 cggccgggtg gcgcggatcg agcaactgga gcatgacgcc gacgaggtga cgcaccagtg
    15121 catcgatgcg ctgcacgcca ccttcatcac cccgatcgat cgggccgata tccaccgcct
    15181 gatcaagcgc atcgacgaca tcatcgactg cgtcgactcg gccgcggcgc ggtttcagct
    15241 ttacggtctg accgtgatgc ggccggaaat ccggcagctc acccaggtcc tgatcgcctc
    15301 ggtgcgcgag ctcgacaaag cggtgcgcga tctccccgcg ctcaaccgta aggccgaggc
    15361 gatccaggaa tgctgccagg cagtgtacga ggcggagcgg gaggccgacg acctcttgcg
    15421 ggcggccctc gtgcgcctgt tcgcagagga aaaggacgtg ttcctggtga tcaagtggaa
    15481 ggagatgttc gagcgggtgg agcgggcgac cgatcgttgc caggaagtcg cgaacatcat
    15541 ctcgggcatc gttatcgagg catcctgagt cgatggaacc gcttcagatc gtcgtcatcg
    15601 gggcaatcgg cgcggcgctg gttttcgata tcatcaacgg attccacgac gccgcgaact
    15661 cgattgcgac ggtcgtgtcg acgcgcgtgc tcaacccgcg cctggcggtc ctctgggccg
    15721 ctttcttcaa cttcacagcc atgttcgtct tcgtcccccg cgtggccgac acggtgtcca
    15781 agatcgtgcg catcgacggc aaagacaccg tgtatgtcta cgtcgtgctg gccggcctga
    15841 tcggggcgat catctgggac ctcatcacct ggtggctcgg tctgcccacc tcgtcgtcgc
    15901 atgccctgat cggcggggtg gcgggggccg ggctggccta ccgggggctc gatgtgatcc
    15961 gctgggacaa cgtctggaaa accgtccaat tcatcccgct ggcgccgatg atcggcctcg
    16021 tcctgggatt ctctttcatg ctcggcgtgt tctggctctt tcggcgctgg cggccgaatt
    16081 cggtcgaccg gctcttccgc cgcgggcaac tgctctcggc gtcgctgtac tcgctcgggc
    16141 acggcgggaa cgatgcgcag aagacgatgg ggatcatcat ggcgctcctc atcgccggcg
    16201 ggatgatgag ccccgatacg aaactatcgc tgggcaattg ggatacgatg tggatcatcg
    16261 tgtcgtcgaa cctcgcgatg ggactcggca ccgcgttcgg cggctggcgg atcgtcaaga
    16321 cgatggggat gaagatcacc aaactcaagc cggtcggcgg attctgcgcg gagacggccg
    16381 gagccgcgac cctgtttgtg gccacgcact gggggatccc ggtgtcgacg acccacacga
    16441 tcacgggggg gattgtcggc gtcggcgcga ccaccaacct gtcggggatc aagtggggag
    16501 tggtgcagcg ggtcgtctgg gcctggatac tgacgctgcc ggcggccggg ctgatcgcgg
    16561 ccggcagttt ctggatcatg cagcacctgt tcatcagccc ctgacccctc cgggatccgc
    16621 ggcgtcggcc gccggtcccg aaaggtgaga tcgccaagaa atctttcgcc ggcgggagac
    16681 ggacggcttg cgcgcgggag ggccgcgcct ctatattagc ccaccggaag aaaagcagcc
    16741 gaacatgaca ccacacattc ccgcccccgg ttcccgacgc cgacgcccgc tctatctcaa
    16801 gattctctat ggtctgcttc tgggtgtcat tggcggcgtg gcggccaatc tcgcggcggg
    16861 cggaaccgat ccgacggtcg cgtgggtgat tcaccatgtg accgaacccg tcggacagct
    16921 gtttctgcgg cttctcctga tggtggttgt gccgctggtg ttttcgtcgc tcatcgtggg
    16981 ggtggcgggg atcggcgacc tgcgcacttt gggacgtgtc ggcctgaaag cgctcgccta
    17041 cacgttcgcg atctccacca tctcggtcgt catcggcatc acgctgagca acatgatccg
    17101 gccgggggag cgggtcgacc cggccacggc aacggcgctt gagtcgcgct atgggacgga
    17161 ggcggccgaa cgggtggagg cggtgcagac ggggtcgggg gagtccccgc tgatgcgggt
    17221 ggtgcggacc attgtgcccg cgaacccgct cgcggcggtg gggggggaga cgccgaacat
    17281 gctccacctc atgtttttcg cgctcgctct gggcatcgcg gcgaccctcg tgccggccgg
    17341 gctggcgcgg ccgtttgtct cgctcatgga gtcgctcttc gggctggcgg ccaaactgat
    17401 cgacctgatc atgctcctgg cgccgtatgc ggtcgcctgc ctggtgttca ccaaccttgc
    17461 tgaattcggc ctcgacctcc tcggcgccct cggctggttc atcgtcaccg tgctcctggg
    17521 gctggccctg catatgttcg gcgtgtactc gctgtcggtc tacctgttgt cgcggctcaa
    17581 ccctctgtac ttcttccggc ggatccgcac ggtgatgctc accgccttct cgacctcgtc
    17641 ctcgaacgcc accctgccca ccgcgctggc cgagacgcag cggaacctcg gcgtgccgcg
    17701 cgaaatcagc acctttgtgc tgactgtcgg ggcgaccgcc aatcagaacg gaacggcgtt
    17761 gtacgaagga gtcacggtgc tgtttttggc gcagctctcc ggcgtcgatc tgagcctcgg
    17821 gcagcagatt ctcgtcgctt atatggccat cctcggcggg atcggcacgg ccggcgtgcc
    17881 gtcgggatcg atccccttca tcgtcatcgt cctggcgacg atcggcgtga acccggccct
    17941 cattgccgtg atcctcggcg tcgatcggat tctcgacatg tgtcggacca cgctgaatgt
    18001 gaccggcgat atcacggcgg cgacgttcgt ggcgcgatcg gaggggtacg agttcgcgcc
    18061 gagcgggggc aatggggatc ccgcggagcc gtcggctcac ggacccgagg cggccgagga
    18121 gggagggtca ggcagccggt gatgacgacc gcgtttgaca ccgccatcct gggagctacc
    18181 gggagaccgg tgcggcggct ggggctctcg gcgtcgtact ggccggggaa aaaaacgatc
    18241 tacgaggcgg tcgactgcgg agtaaccctg tttttcgctt acggattcga tttccaaatg
    18301 atccgggcgc tgcgcaatct ctttagaacc ggccgcgacc ggttcgtcgt ggtcaccggc
    18361 gcgtacaacc tgatctggac gcacaccgac gtcagaaagt cgtgcgagaa gcggcttcgg
    18421 cagctcggca cggactatct cgactacttc cttttcctgg gcgtgatgaa accccgcgag
    18481 ttccccgcgc gggttcagga ggatctgctg cggctgaggg aggaggggaa ggtccgggga
    18541 gtgggggtgt cgtgccacga tcggcgcttc gccgggcagc tcatcgcgga ggggagggtg
    18601 gacactctga tgatgcgcta cagcgcggcc caccgcggag cggagacgga catattcccc
    18661 catgtggcgg cccaccatcc gaccgtgatc agctacacgg cgacgcgctg gcggcaactg
    18721 ctgcggcggc ccagagggtg gccggcggac cggccgattc cgaccgcgcc ccagtgctac
    18781 cggttcgtgc tgtcccatcc ctgtgtggac gttgcgctga ccgccccctc gaatgccgcg
    18841 cagttgcggc aaaacctgct cgccctcgag caggggccgc tggcggacga ggaaatggcg
    18901 ttcatgcggg aattcggcga cgttgtccac gggcgaaggc cgtggtttat gtgaggcgcc
    18961 cgccgcgtcc gggcaaaatg aagccggccc gctcgtccga gcaggccggc gtgttttcat
    19021 ggtctcgtgg gaagagacgc ccagccgctc aggccggcac gctcgccttc ttcacctgct
    19081 tgcccagcca ctcggtgctg accgacttcg ggcgggtgat agcggtgccc agggcgcggt
    19141 tgagcacgag ctgggagagg atccccatgg cgcgcgacac gctgaagagc accgtgtagt
    19201 actcgaattg cgtcaggccg aacgggtaga gaaccgaccc cgaagcggca tcgacattcg
    19261 gccacggatc cttcgctttc ccctgctcct tgagcacgtc ggggaccacg ttgaagacgc
    19321 ggttgacggt ctggaagacc ggatccttgg cgaggtactt cttgccgaac tcgatgaacg
    19381 cggtgaagcg gggatcggtg acgcgcagga cggcgtggcc gtagccggga accactttgc
    19441 cgctgttgag cgtctcccac gcgtacttgc gcagctgctc ctcgctcggg gcgccgccga
    19501 atttctcgat ggtctcaagc acccacgcga ggcactcctg gttcgccaag ccgtggagag
    19561 ggccggccag accgttgaga cccgcgctga gggcgtagta gggatcggag agcgcgctgc
    19621 cgacggtgtg ggcggagaag gcggagacgt tgccgccctc gtggtcgcag tggagcacga
    19681 ggtacagccg catcatgcgc gcggtatccc ccttgtgcgg cggcaaaccg agcatcttga
    19741 catagtcggc cgcccagtcc tctttcttat tgagctcgat gaggtcgccc ttgaaatact
    19801 tgatgcggta gaccgcggcc gcgatcccgg tgatcgtgcc gaggatgcgg atcgagtcct
    19861 cgagtgccgg ctcccagtag acgtccttct tcatcccatc ggtgtagcgc ttgcggaact
    19921 gcgactcgtt ctccatgacg agaatgcccg tgtcgagcat ggccatcggg tgcgacgtct
    19981 tgggcatcgc cttgagaact ttgaggacgt acggcggaat ctccgcggcc gcgcggagtt
    20041 ccttctgcag acccttgagc tcgtccgcgg tcgggagatt gccggtgcag aggagccaga
    20101 agatctcttc gggcagacgg tcggtcagct tgccgatcgg gatgccgcga atgatgaggc
    20161 cctggtcggg ttccacgacc gaggtgtcgc acaccagacc cttgacgccg cgcatgccgc
    20221 cgaacgcctg tgcgattgtg acgtcggaaa tcttgacatc gccgtgctcc ttggcgagtc
    20281 tcgtgcgctc ctcgcgccac ttgggaatga gatcggcgag agtttccttc aatgttttgg
    20341 ccatcgattg ctccttttaa ttcttgaagg tttaacgcga gaggatatta ccgttttgtc
    20401 aggtccccaa tatgtgaaag gtttcacatt aacgggcggt agtgtaacac ctggctgcag
    20461 gcttgtcaag tggggtcggg caaaaagccc gaaagggggg tcatttttcg aagattttgc
    20521 cgcgcccgag aatgtcatgc gggtcgaaaa catccttgaa cttctgcatc gccgcccgcg
    20581 tggccggatc gaattcaaga ctgagaaagc ggcgttttgc cagtccgatc ccgtgctcgc
    20641 ccgacaacgt cccgcccaac gcgatcgtcc ggcgcatcaa ttgctcaacc cggcgcccga
    20701 tctcggcccg gtccgcctca gagccggtca tgctgatgaa gttgacatgc aggttgccgt
    20761 cgcccgcgtg gccatacgag ttgatccgca gggggctggc cgcgttcatc tccatcacaa
    20821 acgccaccag cgacgggaat ttggagttcg gaaccgccac gtcttcggac acgcggatcg
    20881 cggccatcgc tttgagggcg gtcgagatgt tgcgtctgac gtcccataga gtttcggccg
    20941 cctgggcgtc cggctcgaca cgcaggtacg aggcgcggtg acgttcgcag acagcccgga
    21001 tgtgcgcagt ttcctcttcc gggtcgcgct cggtggtctc gatcagcagg acagcggcgg
    21061 cgcgatcgat cccttcggtc ctcctatatg cgttggcggc ggcggccgca tctccgtcga
    21121 catactccag aaccgtgggg ataaggccgg agccggtgat ctcagccacg gcccgggcgg
    21181 catcctctgg ttggcggaag gcgaccagga tggttattcc cgtggcgccg gcgaggatga
    21241 gacggagagc tatccgggtg atgatggcca gtgtcccctc ggaaccgatg aggatgtcca
    21301 tgaggtcgaa gccgcggttc tgattgaaga taccggtctc gaggaggcgg ccctccccgg
    21361 tcaccccccg cacgcccagc acgtagtcgc gggtgacgcc gaaccgcttg cagcgcaggc
    21421 cgcccgcgtt ctcggcaacg ttgccgccca gggtcgactg gtcgaatgac gccgggtcgg
    21481 gaggataggc cagaccgtgg acgagggcct gatcctgaag atgtttggtg atgacgcccg
    21541 ggccgcagaa cgcaatgcgg cgggcggggt cgatcgacag ctcggtcagg cgctcggtgg
    21601 acacgacgag ctcgccgttg gccggaacac accccccgga gagcccggtc ccagcgccgc
    21661 gtcccacgag ggggaggccg cgctcggcgc aaaaccggac ggccgcaacg acatccgcct
    21721 caccggcggc aaagacaatg gcggccggcc ggccgcgaaa atcggtcgcg tcatggtggt
    21781 atgcgtcgtg ggcctcgacc tcaaggacca aggcttccgg agaagaaagt aatgagcgca
    21841 gggccgtaaa gtcggaagcg cgcatcgggg gccgtctcct ggtcgggcgt ttgcgaaaaa
    21901 gggcgtgccg gcgattgtgc ggctctccgg cgccgccgct tcggcgctca cgaattttcg
    21961 gtcttggtcg ttatgccgga cgtttctttc tgggcggcgt ccttgtagtc cttcttgcga
    22021 taatcggtaa tgtagaatcc gctgcccttg aagaggaaac cggccccgcc ggaaatcacc
    22081 cgctcgacat agccctggca tttcgggcat tcggtcagag gcagttcgct gatgcgctga
    22141 aactcctcga aacggcagcc gcattcgtta cacacgtact ggtaggtcgg catcggtcgt
    22201 ccatcactcc ttgtcaatca gctcacagat tctcgcccgg tggcctgtcc ggccggggcc
    22261 gagacaggcg caccgacaaa tatatcacat tttacacgga attagttccc atttcgtacc
    22321 cttgggcgga gctgggcgac gccctctccc agccgtcgca aaccctcgac gagtcggtgc
    22381 tcggccgcgg cgtaggatat acggagatgg ttttccgccc agaaagcgga acccggcacg
    22441 acggccacgc cgtgccgctc aagcagaaag agggccaggt cggcggaggt catcatcggg
    22501 cggtcgccga ctttgccgcc gaggaggccg gaaatgtcgg ggaagaggta gaaagcgccg
    22561 tcggcgttcg gacaggtaat gccggggatg gcgcggaggg cggccagggc ccggtcgcgc
    22621 tgggtctgga gccggatcac caggtctttt cggaagaagt ccggctcctc cagcgccgcc
    22681 agggcggcat gctgagagat cgacgaggcg ttgttggtta cgtgcgactg gaccagagtc
    22741 atcgcctcaa tcacttttcg cggaccgacc gcgtagccga tgcgccaccc ggtcatggcg
    22801 tagctcttcg agaagccgct cacaatgatc acctggtcgg ccagatcggg agccaggcgg
    22861 agcaggcgat acgactcggc gggcgggtag acgatcgcct cgtaaatgtc atcagttatt
    22921 acccagaggc cgcgttccct cgcgacggcg gcgacggccc ggagagcgct ctcggaacaa
    22981 acggcgccgg tcgggttgtg aggagaattg acgatcacgg cgcggctgcg gtcggtgata
    23041 gccgcgcgga gggcgggttc gtcgatctgg aaaccgtcct cgggccgggt cggcagcagc
    23101 accgggacag cgccggccat cttgacctgt tccgggtaac tcggatagta cggtgagggg
    23161 atgatgacct cgccgccggg tccggagatt gcatagaggg cgttgaacaa ggcctgcttg
    23221 gagccgctgg cgacgacgac ctggtccggc gcgaaacgga ggccgtgagt ctcggagacg
    23281 aatcgggcga tggcggtgcg gaggtcgcgg atgccggagg acagcgtgta cttggtgtgg
    23341 ccctcgctga tggcggccat cccggcgcgg gcgacgtgtt cgggcgtggc gaaatggagc
    23401 tccccggtgg ccagggagac gatgtcctgc ccctcgtgca ccaggtcgcg cacgcgggaa
    23461 aaatacgcag tcgtccccga gggccgaagc aacgccatat tcgacgagag cttcatcgct
    23521 gccatcttcc ggtcatgcca aggcgaaacc gaaagcggtt tcgcctggca gacacagaca
    23581 ctgtacgggc cgcccgccgc cagtgtgagg cagtgcggcg gcggaagtat caggcgccgc
    23641 gcaacgcgct gatgccctct ttggtcggac ccgccttgac gacctcgggc ggagtgccat
    23701 cggcgaactt cttgaagttc tcgatgaaca gtgccgcgag ctgcaagtac ttgtcctcgt
    23761 atgctttcgg gtcttcccac ccttcgatcg gccgcagcac tttgtccggc acgccgggac
    23821 aggacttcgg gacttgaaag ccgaagacgg ggtcggtgcg gtattcgacc tggctgagag
    23881 tgccgtcgag cgccgcgttc agcagggtcc gggtgtggtg gatgctgatg cgcttgccga
    23941 tgccgtacgg gccgccggtc cagccggtgt tgaccagcca acaggtcgcc ccgtacttga
    24001 gcatcttgtt gcgcagcatg tcggcataga acgccgggtg gtggaccatg aagggcgccc
    24061 cgaaacaggc tgagaaggtg atctccggct ccttgcccag atcgacctcg gtccccgcga
    24121 ccttggaggt gtagccggat atgaagtggt acatcgcctg ctccggcgtc agccgcgcga
    24181 tcgggggcat cacccccgag gcgtcgcacg tgagcatcac gatgttcttg gggtggcccg
    24241 cctgcttgtc gggtacggcg ttgtcgatgt actcgagcgg gtaggcggcc cgggtgttct
    24301 ccgtgagacg ctcatcgtca aggtccagaa ggcgcgagac ggtgtcgaaa gcgacgtttt
    24361 ccaggatcgt cccgaacttg cgcgtgcagg cgtaaatctg cggctccgcg gtcggggaga
    24421 gcgcgatcac cttggcgtag cagccgccct cgaaattgaa gatcccttcg tcgctccagc
    24481 cgtgctcgtc gtcgccgatg aggccccgct tcgggtcggc cgacaaggtc gtcttgccgg
    24541 tcccggagag gccgaagaag agcgccgagg cgccgtcgtc gccgacgttg gccgagcagt
    24601 gcatggagag cacgccttcg agcggcagca ggaaattcat gacggtgaag gcggacttct
    24661 tgatctcgcc gccgtaaccg gaattgccga tgaggcagag cttttcgtcg aagttcagca
    24721 ggatgaaggt ctcggacagc gtgccgtcaa tctgcggcag ccccttgaac gaggggatgg
    24781 agacgatcgt gaactccggc acgaagcggc ggagttcgtc gttggtggcc ggctcgatga
    24841 acatgttgcg ggcgaagagg gagtgccagg cgtactcggt gatgacgcgg atggggagcc
    24901 ggtagttctt gtcggcgccg cagtagcagt cctgcacgaa gagatcgcgc ccctggagga
    24961 aaccctggag gcggttccac acgagatgga acttgtcgag gctgaagggg cggttgtatt
    25021 cgccccacca gatgcggtct tcggtggagg gttcgcggac gacgaacttg tcgttggccg
    25081 accgggccgt gtgtttgccg gtgctcacgg tgagcgcgcc gccgtgggtc actttcccct
    25141 cgccgcggaa gatggcctct tcgtagagag cctcgctcgg gaggttccag tagacgttgt
    25201 ggaggttggt gaagccgtgg ttgtccaggc cgtattcgct cttgaccgcg ccggcctcct
    25261 tctgggccgg ggtcttgatg tcgatgatgt actgggtcat ttccaatccc tccgcatttt
    25321 gcggtcgccg atgtagattt cgttgggcga ctcgggatcg agagcccact tgatgcgggc
    25381 cacaccttcc ttgatctcct tgatcgtgcc gcagaaggag aggcgcaggc ggccctcaag
    25441 gccgaactct ttgccgggga cgctgacaac ccgggcaaca tcgaggagga acttcgacaa
    25501 cttgaccgaa tccttctggt aggcgcggaa atccggcagg cagtagaacg tgcctttggg
    25561 cgggtcgatg acgacgccgt tgaaggcccg cagctcgttc accatcacct tggcgttgtt
    25621 ctccagcgtg agacggaggc tctcgacgct gctctggacg cccatgaggg cgccggccgc
    25681 ggccgcctgc gcgaccaccg acgggcacga ggtgttctgg gccgaaatgt tgatcatcgc
    25741 ggccgtgatg ttctggttgg ccaccgtcca gccgatccgg aacccggtca tcgcgtacga
    25801 tttcgagacg ccgttgacca cgatcagacg ggagttctcg gactggtcct tcgcatactt
    25861 gtagcagggc gtccacttgc cgtcggaaaa caccagcttg tggtagatgt cgtccatgat
    25921 caggtagatc cccttcttct cgcagtactg gacgatctcg ccgatgaagt cctcgctgta
    25981 gacggccccg gatgggttgt tcgggctgtt gatgacaatc gctttcgtgt acgaactgac
    26041 ctgctgggtg atgtccgcca tggtcggttc gaagcggccg ctctcggggg tgacgatgac
    26101 cgggacgccg tagaccatgc ggacgatttc cgggtagctg acccagtagg gggcgacgat
    26161 gatgacctcg tcctgggggt tgacgatggt catcatgagg ttgtagaagg cctgcttggc
    26221 gccggagaag gcgatgacgt tgcgggggcc gacaaccttg ttgtagttct cctcggtgta
    26281 ccggatgatc gccttgatga gggaggggat gccctccgtg ggggtatacc ggatttctgc
    26341 cgaggtgagt ttggccgcgc tggcgagcac ggcgtcgatg gggactttgc tcttgggctc
    26401 tccagcgccg aggtgaatca cgggttcgcc gcgctctttc aggaggcggg cggcctcgtt
    26461 gagcttgagc gtcggcgact ccttgacttc ccgggccagt tgactgatgc tcatggtcct
    26521 accctcgtaa aagtgtcgcg atcgcgctga cgtcgacgat gtcatcggcc gaacagccgc
    26581 gcgagagatc gttggcgggt ttggcgagtc cctggatgat cgggccggtg gcggtggcct
    26641 tggccaggcg ctgggtcagc ttgtagccga tgttgcccgc gtcgagatcg gggaagacca
    26701 ggacgttggc gctgccggcg atcgccgaac cgggggcctt cttcgccccg acttccggca
    26761 cgatcgcggc atccagctga aattctccgt ccgccttcag atccgggtgc tctttcttca
    26821 acagggccag cgcggccagt accttgtcga catcctcgtg cgcggccgaa cctttggtcg
    26881 aaaaggacag cagggcgacg cgcggctcct ccccgaggag gaagcgcaga gtgtcggccg
    26941 aggacacggc aatcgaggcg agctgctcgg gcgtcgggtt gggcacgacc gcgcagtcgg
    27001 cgaacacgaa caccttgttc ttctctcccc ggtattccgg gatcgtcatg agaaagcagc
    27061 tcgagacggt ggtgatgccg ggtttcagcc cgaggacgtg gatggcggcg cgcaggacgt
    27121 tgccggtggt gttgacggcg ccggcgaccg aggcgtcggc ccggtcgtgg cgcacgagca
    27181 tggcgccgaa gtacagctcg gtggccatct gctcgcgcgc ctggtcgggg gtgagcccct
    27241 tggccttgcg catctcgtac agctcgtggg catatgcctc gaactcgggg tgccggcgcg
    27301 gcgtgatcac ctcgacggcg ccgagctgca gcccccggcc ttttgcctcg gccgcgattt
    27361 tgtccgggtc gcccaggaga atgacccgcg cgatcttctc ggcggtgagc ttcttggcgg
    27421 cctcaatact ccggggctcg gcgccttccg gcagcaccac tgtcccgtgt ttcttccggg
    27481 cacgttcctt tatgagcgat acggcatcca tgatgcactc ccacagttcg actattgtgt
    27541 attcccggtc ggcgcgagcg ccccgagact gtcattgagt tccggatcag tcagaaaagc
    27601 ctggatgaac tcctccaggt cgccgtccat caccgcctgc acgttgcccg tttccaccga
    27661 tgtccggtgg tccttgacca tctggtaggg ctggaagacg tacgagcgga tctgtgaccc
    27721 ccactcgatc ttctttttgg actcctcgaa cttggccatc ttcttggcct cctcctcccg
    27781 tttgagctgg tagaggcggg acttgaggac ggtgaaggcg gcctctttat tcttgtgctg
    27841 gctgcgctcg gactggcaag tcaccacgat cccggtcggg aggtgggtca gccgaatggc
    27901 cgaggatgtc ttgttgacgt gctgcccgcc ggcccccgag gaacggtagg tgtcgatccg
    27961 cacctcctcc tcccggatct cgatttcgat gttgccctcg acttccggga agacgtgcac
    28021 ggagacgaag gacgtatggc ggcgggcggc tgcatcgaac ggggagatgc gcacgaggcg
    28081 gtggacgccc gattcggctt tgaggtagcc gaaggcgtag tcccccttga tttcgagcgt
    28141 cgccgatttc agtccggcct cgtcgcccgg ctggtaatcc atgagttcga cggtgaagcc
    28201 gttgcgctcg gcccagcggt tgtacatgcg aaagagcatg tccgcccaat cctgcgattc
    28261 ggtgcccccg gcccccgggt ggatggagag aatggcgttg cggtggtcgt ccggtccgga
    28321 cagcagggcg gccgattcaa tgcgggcgag ctccttctca acgccgtcca gggtcgcttc
    28381 cagttcgacc agctcgcccg ggctctccac ggcgtcggcc agctccagca gttcctccac
    28441 ggccgtgaga tcggcggcga ggcgctcgta actctcaagc caccggcgat ggaccgcgag
    28501 ttctttcagg atcccctggg cgcgctggtt gtcatcccag aagtccgggg cgccggaagc
    28561 gtcttcgagt tctttcactt tgtctcggcg tgcccccagg tcaaagatac ctcgcaagct
    28621 tttcgaggcg acctttcagt tcggggacac ggatcttcaa ttctgccagc atcgcgccgt
    28681 cacctccgta tctggaaggg tcaaatataa gcggtcctct cggaagggtc aacacgaatc
    28741 ggggagagat gttatttttt tcacaagctc cgcgggcgcg cgccgcgcac aaaagggcgt
    28801 tgacaagctg ctctccgccg ctatcttggg accctgatat gcaagtacaa acactcgtcg
    28861 tcggcgcctt tcaggtcaac tgctatctgt attggggcga cgcggccgga cgcggggtca
    28921 ttatcgaccc cggcgatgaa gaagagcgga tcgcggacgc ggttacgaag gccgggtttg
    28981 agccggccgc ggtgctgctg acgcacggac acggcgacca catcgcggcg gtgtcgccgc
    29041 tgatgaagca gtacggaatt ccgctctggg ccggagccgg cgaggagaag ctgctggcca
    29101 atccgacggc gaatatctca gccctcgtgg ggcgcccggt gacgaccccg gtgccggaca
    29161 agctgttgcg ggacaatgag gtggtgtcgg cagggccgtt tgacctgcgg gtgctcgcga
    29221 cgcccgggca ttcgcccggc ggcgtgtgct acctggacga gcgggccggc gtgctcttca
    29281 ccggcgacac cctcttctgg gggtcgatcg gccggaccga cctgcccggc ggttcccaca
    29341 cgcaactgat ccggtcgatt cgggcgaaga tactgacgct gccggacggg gtgatctgtt
    29401 accccggcca cggacccacg accacggtgg gcggcgagcg tgtgaacaac cccttcctga
    29461 tggatgatta tgtctgagcc gaacatcgcg cggctggcca cgccgcacct gaccgacttc
    29521 cacgtccact gcgattattc gaccgacgcc gtggggacaa tcgacgagta ctgccgggcg
    29581 gccctcgccc gggggctggc ggagatctgc ttcgtcaccc actacgacac gaacccggcg
    29641 gtcgattccg gcgactgttt cgtgcggatc aaaggggtcg aacggcgggc cgccccggaa
    29701 cacctggccc cctatgtcga cgaggtgcac cgggcgcacg acgaatacta cccccgcggg
    29761 ttgatggtca aactcgggat cgaggtcggg tggtgggaag gctgcgagga cacggtgcgg
    29821 ggggcgatcg agcgctaccc cttcgactac gtgctgtgcg gcattcatga tgtcggcgac
    29881 gtctgcatct gctcgcacga caccgagggg aatcttgggc gcatggcgcc cgagcggctc
    29941 atcgaggaat actttcgcca ggcggtgctg gccgcgcgca ccgggctgtt cagcgctctg
    30001 gcgcacctca cctactaccg gcggttcggc gagcagtact acgggagcat catcaacgag
    30061 ctccaccggc cttacctgcc ggagctgtgg gaggcgctcc gcgccggggg cacggccatg
    30121 gagatcaata cctcggcgat ccgccacggg ctgggggaat actatccgcc gcccggcctc
    30181 atccacgcgg cccggcgggc cggcgtgcgc gtcgagcgcc tggggtcgga ctcccaccgt
    30241 cccgaacagg tggggcttga tttcgatgcc gcggcgccgc tggtggccga gcaggcgccc
    30301 tactacggcg agtgaggtcc cgggtggcgg cgacacgttc cccgaaaaaa ctcccccgct
    30361 cgttctacgc ccggcctaca ctcgaggtcg cgcccgatct gctgggcaag ttcatcgtct
    30421 accgctcgcc gcgcggccgg ctggccgccc ggatcaccga agtcgaggcc tacatcgggc
    30481 aggacgaccc ggcgtgtcac gccagccgcg gaaaaaccgc gcgcaacgag gtcatgttcg
    30541 gaccgcccgg tttcagctac atctacttca tctacggcat gtactactgc ctgaatgtcg
    30601 tgaccgaacc ggagggacag ccggcggccg tgctggtgcg ggcggccgat ccggcagagg
    30661 gcctggatct catcgcccgg cgatcctcgg gaaacggacc ccacgcctgg ctggcgggac
    30721 cggggaagtt gtgccgggga ttcggactga cccgccggca aaacggcatt gatttgaccc
    30781 gtgatgttct gtatcttgag gaccgccacg cgccggttga ggcggtcggc cggtccccgc
    30841 gcatcgggat ccgcgtcggc gccgaacgga tgtggcgatt ctacgaccaa aatgccaagt
    30901 ccctgtcccg gccgggataa ccgcagtgaa gagaggtgag gaaccgtggc caagtcatcc
    30961 cctgtcaagg cgagcgagac atcgacaaag aactccggca aacccaagcg gcgcgaacac
    31021 cctcgcagcg tggtgtccgt ccgcgaccgc accatcccca tttcggtctt cggcaacgtg
    31081 atgaagcagt tcgacatggc ggccgcgaag ctgaacctcg aaccggaact gctcaatttc
    31141 atcaaactcc cccgccgcag caccatcatg cacctgccgg tgcagatgga tgacggcacc
    31201 tggcggatgt ttttggccta tcgcgtgcag cattcgatcg cccgcggtcc cgccaagggc
    31261 gggatccgct accatccgga tgtcacgctc gacgaggtgc aggcgctggc ctcgtggatg
    31321 acctggaaga gcgcggtcgt caacatacct ttcggcggcg gcaagggagg gatcgtgtgc
    31381 gatccgacca agctctcgcc gagcgagctg gagcggctga cccggcggta cgcggcggac
    31441 atggtggacc tgttcggtcc ggaccaggat gtgccggcgc cggacgtcgg caccggcccg
    31501 caggtgatgg cgtggttcat ggacacctac tcgatgcggg cggcgcaccc ggtgccgggc
    31561 gtggtcaccg gcaagccgct gcagttgggc gggtcgcgcg ggcgcgtcga ggcgaccggg
    31621 cgcggcgtcg ccctctgcgt cagggaagcg gcgcgctacc tcggcttcga acttggccgc
    31681 tgcaccgccg ccgtgcaggg gttcggcaac gtcggttcga tctcggccaa actgctgcac
    31741 gagctggggg tgaaagtcac ccacgtctcc gacgtgaacg gcgcgctccg cgatccgcac
    31801 gggctggata tcgaggcgct cctcgagtac gcccaacaga agcggacgat cgtgggattt
    31861 ccccgcgccg aggctatcga ggcggccgac gttctggcgg ccgaggtcga tatcctggtg
    31921 ccggccgccc tggaaaatca gatcacgcac gcgaatgcga agactgtgcg ggcgaggatc
    31981 atcgccgagg gcgccaacgg accgaccacg ccggaggcgg acgcgatcct gaacaagaaa
    32041 gggatcatgg tcatccccga cattctcgcc aacgcgggcg gcgtgaccgt gtcgtacttc
    32101 gagtgggtcc agaaccggat cggctatttc tggtcggagg aggaagtgaa ccgtcggctc
    32161 gaggagaaga tggtgatggc gttcaacgac gttctgcggg tagcgcagga acaccatgtg
    32221 gacatgcgca tcgccgccta catgctggcg attacgcggg tggtcgaagt cgtgcgcctg
    32281 cgggggatct acgcctgaac cgggcgcccg ctgacaggcc gcgtcggggg gacagctccg
    32341 cgtcgggttt ttggtggccg tcgggccgac cgcgccgtat aatgtgcatg tcgtcgggac
    32401 gcttcaaccc ggcggcgcgc gagattatgt gatggatgcg gactttctcc agcgggcgct
    32461 cctggcggga ccggcgatcc tcttttcgct gaccttccac gaattctcgc atgcctacct
    32521 ggcctactgg ttcggcgaca ccacggcgcg ggacctcggg cggctgacct tcaaccccct
    32581 ggcgcatctg gacctcttcg gcacgattgt gatggttttc tcgcagttca ccttcggctg
    32641 ggcgaagccg gtgccggtca acccgtggaa tctgcgcaac ccgcgccggg cggatttctg
    32701 gatctcggca gccgggccgc tgtccaatct taccctcgcg ctggccttcg gatcgctctt
    32761 ccggatcctg ggtccgcacg cggggtctct gcccgaagcg gtcccgttgt ttcttatgtt
    32821 cgccgtgcag gtcaacatcg ccctggcagt tttcaacctg attcccctgt ttccgctgga
    32881 cggttcgcat atgcttcgca atgttcttcc cgaggaaatg gggtcggcgc tggactcttt
    32941 cgaccggatc gcgccgttcg tgctcctgct gctggtggtg accggagcgg tcagtgtcat
    33001 ccttgtccca atcatgcggg tcctcatccc gctgcttctc ggagcatgag atgcgcacgc
    33061 ggcggggcat agtcatcatg ccgtcggcgg cgctcgcgct tgtcctgtcg gccggctgcg
    33121 ccggcgggta ccggggaggg gcgccggaaa cgcgcgccga cctcgatcgg gtggaagtca
    33181 ccgccgacgc ctacctcttc gatgcgcgcc tggtgcggga ggggaagccg acgtcggtgc
    33241 ggttggagat ctactacacc gacacgctga tcggtctggg tggacggggc tatctcggca
    33301 aaggggcgct caaaggatgg ctgcgcgagg attcactgag agtctatttc cctggttcca
    33361 acgagtatgt ctacgaggcg gtgccgacgc tcctggagtc ggacgcctgc gcgcaggcgg
    33421 aggtccgggt gccgcttgcc ggactgcttt cgaccacgcc cgattcgctc ggggcggcgg
    33481 aggatctcga ggtggaggcc gattacgtgg atcccgatga accgcgcttt gtgattcgcg
    33541 ggcggggatg tccctggcgg ctggagatcc gctacgaccg gcgcgacgac cggtggcagg
    33601 tcaaggagtt ccgtttcgcg gacggcgccg acctgtggct gacggcgcag cggcggacgg
    33661 taaagcacgg ggcgaaaatg agcgccggcc ggttcgcggt cgccatccct gaaaacgccg
    33721 tccggatcgt accctgaaac cgccgggcgg ttgacttccc tccccgccgg agcgatattg
    33781 ccgaccggtc actttcttac ggagcaagtc agtgaatctg tatcgtcggg cgctcgcggc
    33841 cctgaaaccc tactggaagc acctggtcac cgcctcggtg tcggcggccc tctcggcagt
    33901 gctcgccggc ctgctggtgt ggatgctcgg cccgcttctc atggtgctgt ttgaggtgtc
    33961 cgatccgatg gcgacggtgg cggcgccgac ggctacggcc gaggtccggc cggcgacccc
    34021 cggcgaagcc accgggaact cggggatgat ccagaagctc tcctcccggc tgggcaaagt
    34081 caaggagtcg atgcaggcct gggtcgacgg tatcgtgcgc tcggacaacc gccgccagac
    34141 ccttctcaac ttctgctggc tggcctttct ggtcgtgatc gccaagaacg tcttcctcta
    34201 cctgcggggc tacttcatgg cctacgtcca gcaagccctg gtgcggcgtt ttcgcgacca
    34261 gttgttcgag aaataccaac ggctgccgct cgggtatttt cacggccggc gcaccggcca
    34321 ggtcatctct cggatcacca acgacgtcct cgtcctcaat gattccgtcg acatcggatt
    34381 caacaatttg gtcaccgacg ccatcacggt gattgtgctg ttcgctttcc tcgtgatcct
    34441 gagctggaag ctgacgctgc tgtcgatgat cgtgatgccg ctcgtgttcg tgttcatctg
    34501 gttcgtgggg aagaagcttc ggaagtactc ggcccgcacg caggagcgga tggcggacgt
    34561 caactcggtg ctggaggagg cggtcaccaa cctgcggatc gtgaaggcgt tttcgaccga
    34621 ggagttcgag accgggcggt tcttccgggc cacccaccag tatttccggt cgcttctgcg
    34681 catgacccgg atccggcacc tcgcggcgcc gatcaacgac atgctggcga cgacggccgg
    34741 gctgtttatc ctgctcgtgg cggggacgcg catcatcgcc gggacgggag agctgagcgc
    34801 cggggacttc atcaccttca tcgtcgccat gctcgccatg atcaaaccgg tcaaatcgct
    34861 cagccagatc cacatcaagc tgcaggaggg gatggcggcg gcggaacgca ttttcgaggt
    34921 gctcgacgcc cccgagacgg tgggcgaacc ggcggcgccg gttgtgctgc cggagttccg
    34981 cgaggtaatc cgctacgagg gcgtctggtt ccgctacgaa acctccgacg acgtcctccg
    35041 cgacgtgacg ttcgaggtgc gcaagggaga ggtggtcgcg atcgtcggac cttcgggcgc
    35101 gggcaagtcc accctgctcg acctgctccc gcgcttctac gatccgcagc gggggagggt
    35161 ggcgatcgac gggcatgaca tccgcacgct ctcgctggcc tcgctgcggc ggcagatggg
    35221 gattgtcacc caggagacgc tcctgttcaa tgacacgatc gccaacaaca tcgcgtacgg
    35281 cttgacggac gtcccgcgcg agcggatcga ggaggcggcg cggatcgcca acgcccacca
    35341 gtttatcgct gaattcgagc ggggctacga cacgccggtg gggaatcggg gggtgatgct
    35401 gtcgggaggc cagcgacagc gggtggcgat cgcccgcgcg ctcctgcgcg atccggcgat
    35461 tctgatcttc gatgaagcca cgtcggccct cgacaccgag tcggagctgc tcgttcagga
    35521 ggcgatcgac cggctcatgc gggaccggac ggtgctggtg atcgcccacc ggctgtcgac
    35581 ggtcaagaat gccgaccgca tcatcgtgct cgaccgcggc cgcattgtcg aatgcgggag
    35641 ccactccgac ctgctgcggc acaacggact gtacaaccgg ctgtacacta tgcagtttca
    35701 ggacgtcgat gcacatactc cagcttgact ccatcgaacc ggaaacctac ggcggtatgg
    35761 aagaatggat tcggctggtc tccggcggat tggccgcgcg gggacaccgg gtcacggtgg
    35821 tcgggcggac gggcgcagag ttccttcggc gggtcgagct cgatacccgg ggcgtagaga
    35881 cgctccacct ggcaatcgcc ggggatttcg gtccgctcgt gatcgcgcgc ctgcgcacct
    35941 tcatgcgcca gcgcgctgtc gatatcgtca ccgtcaattt caataaagac atccggctgg
    36001 ccggaatcgc cgcccgctgg gcgggccgcc cgcgcgtgat ctggcgggtc ggactggaca
    36061 tcgccggaga ccgcctccgg caccgcctcc tcacgccccg cctggtcgac gccgtcatca
    36121 ccccctcggc cgcgctcaag aagcagatcg tcgcccacgg ctacatcagt ccggagatgg
    36181 tcacggtgat tccgaacgga atccccgacc ggccggtgca cgaacagcgg ccccaggcgg
    36241 cgcggcggct gcgcgagaag tacggcctgc cgccggcggc gctggtggcg gtgacctccg
    36301 ggagatttgt cgatcagaag gggcacgcct acctggtggc ggccgccggc gagatcatcc
    36361 ggcaggcgcc ggacattcgg tttctctggc ttggggacgg ccccctcgag actcgtctca
    36421 aagcggccat cgcggatgca ggcctggaga gctttttcgt ttttgcgggt atgctgcgcg
    36481 acttcgacct ggaactggcg gggagcgatt tcatgctcca cccggcggtg gaggagccct
    36541 tcgggttcgt cctgatcgaa gcgatgcggg ccgggctgcc ggtggccgcg acgcgggtcg
    36601 ggggaatccc cgaggtggtt gcggaggggt cgagcgcagt cttgttcccg ccgcgcgatc
    36661 cggcggccct ggcggcggcg gcggtcgccc tggcctgcga ttccgacgcc cgcacgcgcc
    36721 tgggacgggc cgggcgggag cgattcgagc agcggttcag cgccgaccgg cttgtcgatg
    36781 acgtcgagcg gttcttcctg tcgcaactgg aggaggccgg gcagagccat gggacggctt
    36841 aaggagttcg agtactcgtt caagcggttc ttctaccggg ccaccgcgtc gctgctccgg
    36901 ccgcggccgg tcggggaggg accgctcgac ggcgcggcgc tgcggaaagt gctcttcctg
    36961 cgcccggaca aactcggcga catggtcatt tccttccccg tcttcgacgc tcttcggacc
    37021 cggtatcccc acctgcgcat cggggtgctg gcatcgccgc gaaattcggc catcaccctg
    37081 ggcgacccgc gtttcgacga gatctacatg tacaccaagc gggcgccgca cgacctccgc
    37141 gaactccggc gcctccggcg ggcgcagtac gactgcgtgg tcgacctcgt ctacggcgat
    37201 tcggtcacaa ccctgtacct ctcggagtac gtggcgcgcg gcaagcctcg gatcggcgtg
    37261 ggcaaggagc gctacgcccg gtactacgac ttcaaccgct ccggccgggg acagattctt
    37321 gaatgcacgc tccaggtgct ggcggcgttc ggcatcgacc ctgcggccgt taacccgtac
    37381 gcggggccgc atctgagccg cagcgatcag gagttggccg cggcctttct cggcgcggct
    37441 gcggccgacg gcgccggcag tttgacagtc gggctcaatc tttcagccgg gatgccgtcg
    37501 cgcctgtggc agccggagaa atcggccgag ctggcggcgc gcatcctggc cgcccgtccc
    37561 cgggcccgca ttatcgtcat cgtcgtgccc ggcgaccgcc gccgcgggca ggaactggtg
    37621 caggcggtgc gcgacgcgcg ggtgcacctg gtgccggacg gcctcaccct ccggcaggtg
    37681 gccgccgtga tcagccggct tgacctgctc gtcacgcccg acaccagcct cgtgcatatc
    37741 gcgcgggcgc tccaggtgcc ggtggtcggg ctgtacagct gccacgaacg caacttccgc
    37801 cagtggcggc cgtacgggca ggacatcgga gccgtcgtcg cgccgagcga ggacaacatt
    37861 ttcgccatca cggtcgacca ggttatggcg gccttcgagc aggtcgcggc cgggcggctg
    37921 ggcgtggagg aatgatgggt ctgacggttg tggtcatcac caaggacgag gaggccaaca
    37981 tcgagcgctg ccttgagtcg gtggcctggg cggacgagct catcgtcgtc gattcccaat
    38041 cgactgaccg gacggccgag ctggcgcggc ggcgcggggc ggttgtgtgc gacgtggagt
    38101 ggcgggggtt cggcccggcc aaacggcgcg gtgtggcgct ggccacccag gagtgggtgc
    38161 tgtcgctcga tgccgatgaa gtcgtgggaa ccgagctcgc ccaccagatc cgccaggtgg
    38221 ccgccggcgg cggggagcac gacggctact acgtcggccg gcgcaccaat ttcctcggcc
    38281 gctggatctc gcactgcgga tggtaccccg atccggttct gcgcctgttt cgccgcgaaa
    38341 aggggaatgt caacgaggcc gtggtccacg aacaggtgga agtcgacgga tcggtggggc
    38401 ggctgtccgg cgaactcctg cacttcagct acccgactct ggagcactac ctggccaaat
    38461 cggccgttta cacgactctc ggagcgcgcg aggcgtttcg ccggggccgc cgcgcgcgct
    38521 ggtatgatct tgtcattcgg cccccgatct cgttcttttc gcattatatt gtccggcagg
    38581 gattccgcga tggaatggag ggatggatca tttcggtgat gtcggcggtt gcggtctttg
    38641 tgaaatatgc caagctgcgc cacctgcagc ggaccgcgac cccggaacag agggagcagt
    38701 atggctttgg agattgacct gcagccgggg gacaaaatcc tgatcagccg gaccgaccgg
    38761 ctgggcgacc tcatcctcgc gctgcccttt gtcgagacca tcaagcggcg ctaccccgag
    38821 tgccgcgtcg acgtcctggc gtcgctgtac gcctcgccga ttctggagaa taacccgcac
    38881 atcgaccgga tcgtccgggt gcagaacgac cagctctgga ccgacaacct gtacaagaag
    38941 gacctcctgc accggatacg gatggaaggc taccgggcgg tcgtggcgct gtaccctgaa
    39001 cggcagatct cgcagttgtt ccagcgggcc gaaatcccga tccgcatcgg gaccgcccgc
    39061 cggttccact cgatctactt cacccaccac gtcacccaca gccgcaaggc caacgtgaaa
    39121 cacgagtacg agtacaacct cgacttcctc cagttcttcc gcgatggcga gacggtggcc
    39181 agcccgaagg tgtacctgcg ggaacgggaa ctgcgcaacg cgcggcgcat tctcgacgac
    39241 gtcggggcgg aggaatcgtt tgtcgttatc caccccgggt cgcgcgggtc ggccgaacgc
    39301 tggccgcccg agcgctttat cgagctctac tgccagctgt cccgggaggg gctgagcgtc
    39361 gtgctctccg gatcggagga ggagggccag gggctgtcgg atctcaccgc cgggctgccg
    39421 gtggccgtgc gggacatctc cggccagacc gatctgcgga cgctggcggc cgtgctgtcg
    39481 caggcgcggg tggtggtggc caactccacc ggcccgctcc acttggccac cgccgtcggc
    39541 acccacgtcg tgggcctcta cccgagccgc aaggcggtct cgccgcggcg ctggggaccg
    39601 ctcggcgaac agcaccgggt catccagccg accggagtca agtgcgagtg cccccccaag
    39661 gggtgccggt gcatggatac catctcggtg gagagggtgg tcgaggaagt cgtggccctg
    39721 taccacgtgc agtcagtcgg atgacggcga tatgaatctg gccgagttca tcatacggat
    39781 cgaatcgttc aacgccaacg tgaacattcc cctgatccgc aaagcctatg agttctcgga
    39841 ccgggcccac gccggccaga aacggcagtc gggggagccg ttcatcgagc actgcctcga
    39901 agtcgctttc attctcgccg agcagcacat ggattcgacc acgatcgccg ccgggctggt
    39961 ccacgacgtg gtcgaggaca ccaagatcgg gatcgaccac ctccgggccg agttcggcga
    40021 cgagatcgcg gacctcgtcg acggcgtgac caaactgggc tcggtcgaat tcacctcgcg
    40081 cgaggaacag caggtcgagt atttccggaa gatgctgctc accatggcgc gggacatccg
    40141 cgtcatcctc atcaagctcg ccgaccgcct ccacaacatg cggacgctcg aggccctgcc
    40201 gcccgacaag cagcggcgga tcgcccagga gacgcacgac gtctacgcgc cactcgccca
    40261 ccgcttcggg atcaaccgca tcaagaccga actggaggac ctctcgctga aatacctcga
    40321 gtccgaggta tattttgaaa tcgccgcccg gctgaaagaa aagcgggagg agcgcgaggc
    40381 ctacatcgaa caggtggtgc gtccgctgaa ggaagagctg accaagaaca gcatccaggc
    40441 gaccgtctac gggcgggcca agcacatcga ctcgatctac cgcaagatgc gcatccgcaa
    40501 cgtgcccatg gaggacatgc acgacctgtt cgccatccgc tgcatcgtca acaccgagcg
    40561 ggagtgctac cacaccctgg gtatcgtcca cgccatgtgg aaaccggtgt cggggcgctt
    40621 cgacgattac atcgcgaacc cgaagccgaa cggctaccgg tcgctccaca cggcggtctt
    40681 cggccccctc aacaagatcg tcgaaatcca gatccgcacc caccagatgc actatgtcgt
    40741 cgagaacgga atcgccgcgc actggctgta caaagaggga cgccaggaaa tgagccgctc
    40801 cgaccgccag atggtctggt tgcgcgacgt tctcgagtgg cagaaggaca tgaccaatcc
    40861 ctcggatttc ctcgaatacc tcaagatcga cctgtactcc gaggacatct tcgtgttcac
    40921 gcccgccggg aaactgattc acctgcccaa gggttcgacc ccgctggact tcgccttcca
    40981 gattcacagc gaggtcggca tccactgtgc gggggcgaaa atcaacgggc ggctccagcc
    41041 gctctcgacc gaactgcaga ccggcgacag cgtcgagatc atcaccaatc ccaaccgcac
    41101 gccctcgcac gactggctca agctggtgaa aacctcgcag gcgcggtcgc gcatccgccg
    41161 ctggctcaag caggccgggt tcgagcggtc ggtggctttg ggcaaggaaa tcgtggagcg
    41221 caaactcaag gaagagcggc tgaaaatgcc cgacggcgat acgctcctgg gctacgccca
    41281 gcagctggac aagaagacga tcgaagagct gctcgcggcc atcggcagcg gggccatggg
    41341 cgtcggcaaa ctcatgacgc tgatcgagcc cgccctcgaa ccggaagaaa ccggcttcgt
    41401 cggacgggtg atcgaacgca tccgcgggtc gaaggggatc aaggtccacg gcctggatga
    41461 catgatgttc cgcttcgccg gatgctgcca gccgatcccg ggggaggaca tcgtcggatt
    41521 catcacgcgc ggacgggggg tgagcattca ccgggccgac tgcacggtgg cgatttcgct
    41581 gcaggaacag gccccggagc gcaagatcga cgtaagctgg gatacggcca aggggcagtc
    41641 gttcgtggtc cggctcgaga tggtggtcga ggaccgcaag aacatgctcc gcgacatcac
    41701 ccaggccatc gcgaccgccg acaccaatgt ccgcgcggct gaaatgtatg cgcgcgatac
    41761 gacggcggtg ggggaattcg tcgtcgaggt gtcaagcctc gcgcacctga accgaattct
    41821 cgataaagtg cgcaaggtca agggggtgat caaggtggtt cgggcgaagg ggaaggacag
    41881 cctggagaat gtcgagaata tggagtaggg gagcgtgacg attggggccg ggcgaggaat
    41941 tgtcgtcgct gtctacgcca cttcctcgca cagctcggtg gggagaggca caccgcggaa
    42001 cagcacggca atcaggaggg tcacgtcggc aacgtttacc ccacagtcac catttaggtc
    42061 gccgcgcaga attggaatgg gtgccgggcc gccgcgaaag agatagtcga tcaaacgggt
    42121 gagatcgcta atggttagcg tgctgtcgcc atcgacgtcg ccgggcgata ggagtactgc
    42181 gtcaattgcg tgagcggcaa acaagtcctc aaagacagtg ctgaaggaat agcccaggtc
    42241 gagccagccc gctctgaact ctcgaatggt ccagcagtga tcatggtttg ggtctgccgg
    42301 gcagggtcgt agtttgtcag gacatgccaa attgcggcag gaccggccca ccaggaaccg
    42361 ccattgtagt cctggttgtg tccccacaat gcgccgccaa tatattggtt gccgtcgtcg
    42421 gcattgtcat caaggtcaat aagagaaacg gcgacagctc cggggacctg cgcgccgtcg
    42481 tactgcgggt tcaaaggtgg tggcggcaac ggttcgagat tctgattgtc atcgtaataa
    42541 ggcgggttgg gcaacggata ttcaagattc ttgacatcac atgcaggatc ggtgaacgta
    42601 gagttgacaa actccacctc accaagatag ctgcagcctg cgtagaaagc ccgaccttcc
    42661 gtcagtgctg cacgggcatt ctcgctgcca tacagcggaa acaggtaatc gtatggatca
    42721 cctcctccgg gtatccgctc catatagctg aacatgaggt ggtgggcaaa ttcatgggca
    42781 ataacatgac catcccattc atcccaatta tcaaggaagt tcgcgcggct ggcaatgtag
    42841 aatccaggaa ctccatcgta ctcaaatcta ttgtagacgc tcgttgactt tgagccgtct
    42901 agatcgtccc atatcacccc catgtacgga gccaagtctt gctcaccgaa gtcatctacg
    42961 agaagttgct gaagaaggcg aaacatgtcc agtatgttac acgcgcctcc tacagacaag
    43021 tcagtgatct atgtgtatac ttcgggaaca tagacattcg tgtattcagg gttatcgacc
    43081 tggatgccgt gagcatgaag ccaaaacgtt tcatcatcga gcgagaaatc atggttatct
    43141 gcgtacacga tttcggcgga tgcgtttatg gaaacaaaat agaaccgaac ccggcgattg
    43201 ttcgtttgaa agctgaagta gccgctggcg cctatgtcta gctgcctgtc cagagtatac
    43261 cgcacgccat cattggattc gtagatcccc agaccaacgt tgcagatcgg ccattcgatt
    43321 ccgaacttat ttacataatt ggcggtcccc tcattcagta ccatgcggca tcgtgaacaa
    43381 gcagaattcg ttccagcgac ccagcttcca cccgaaacag ggcccgcccc gaatcacctt
    43441 tcaactcgaa aacgccggtt cttgctccgg acttagtata cacagcgttt ccctccagca
    43501 gaaaaacccg aacggatcca gtgaaagcgg agtccgtgaa gaattccacc tcgcgggtag
    43561 gatcgtatgc gattcggatt gtatccggtg actcagagct gagattgatg ggggtcgttt
    43621 gccgcgggcg cagtatcggc ggtccgtcac cgtgcagctg gcgtggcctc ctcgtcccgc
    43681 gtcggttggt ttggtccaaa ctatcgatca ccggatgcct cttgagttca gaaggagcat
    43741 tgcttgctgc cgtgtctgcc aaaatgaggc cagtgtcggt taccttgagg cgccagagac
    43801 ggctgatctg atcgttctct tcagcctggc ggaaatcgat gggtttgctt cgaaactgtg
    43861 caacacactc acggccaaca tctactcctg catttggacc gaattggggt ggaatgatct
    43921 gccccaatgt ctgtgctgcg tgcacccagc ccgtaacgac tcgcttgatc tccgcccgga
    43981 tgacgaaccg agccttcaac tcgacggtct gacaccgggt aagagagcct atccagagag
    44041 tgtctccacc cacaaattcg acgcctggat taccacgaat cagcactgtg tccggtattc
    44101 ccttcgaatg actgaatgag tccacagggg tgaatctgaa caccacgtcg aagggctcgc
    44161 cgagtttggg ctctccgact atctcaactc caccagatcc cttgaggtct ccgtactcta
    44221 aagcgatatt tgcgtgcgtg gtgtcagcga ctgcaaccga caccaaggcc accgacccca
    44281 ttatgagcat gaagattcgt atagtgactt cctcccgtgt tgaatgccag tgcttacgct
    44341 gcttaatatg atgcggggct cccgatgaac cccttagtat ccatagtgaa cacctgatgc
    44401 atagccgttg tcaagctcca tgtttaccgg aaacaggatc cgtgagtact cctttacagc
    44461 actgattcgg tgctcttttg caggcatcag gggcgtcagg gacttgctca gcaacttctt
    44521 ctattcaaag acaactgtca gatccaccgt ctcggcgttc cgattgaccc ggacgacggt
    44581 cgtgtcgccc ttgcggaagc ggccgagcgc gttcatgtag tcgtagatgt ccccgatttc
    44641 gatctcgccc atgcgcgtga tgatgtcgcc ggccagcaga ccggcccgtt cgccgggacg
    44701 gtcgggctgc acgttatcca cgcgaagacc tttgacctcc gcgacgtagt cgggcatgat
    44761 gcccagggtc acggagaacg tcgaggcgcg cttgccttcg tcaggatcct tggtcttctg
    44821 gaatgtcagc gggccgggat agcggtcgaa ataggaaacg agatcggtca cgacgtcggc
    44881 gaccgtgacg aggccggcgt agtcaatcct gtcccaatcg tcctccggct tgtggtagtc
    44941 gttatgggcg ccggtgaaga acatgaggac gggaatgccg cgattgtaga acgcggtgtg
    45001 gtcggagggc ccttggccgg acttcttctg cgtgatcttc acgtcctcac gcctgaggct
    45061 gtcgaagtag acggtgaagg cctcggtcgt gccggtcccc atcaccgcca gcccattgtc
    45121 ctgttcggcc agccgcccga ccatgtccat attgaccatc atgcgaacct tcgcgctgtc
    45181 gacggtcatg tcccgggcga aatggctcga gccgagaatg ccgaattcct cgccgctgaa
    45241 ggccgcgaaa aggagagaat agtgcggttt ctcggcctgc gcggcatagt ggcgggcgat
    45301 ctccagcagg accgaagtcc ccgatccgtt gtcgtcggcg ccgttgtgaa tctgcggatc
    45361 gttgcccaga tagcgcgagc cgcttccggg gccgccagac ccgagatgat cgtagtgggc
    45421 gccgatgatg actgtcgtgt cggtctcgcc cggcaggtag ccgagcacgt tgtagccggt
    45481 gtcgtggacc ttgaccagtt ccaccgcccc ttcggccgac aggacggccg gatcgctcag
    45541 tgagagcccc aggtgctcga atccggcgcg gcgcaggaag atgatgggga tgtctttcgg
    45601 cgtgatccgg accgggccga tcggctggag cgtgtcgtcc tgatcctcgg gggtgatgaa
    45661 gaagataccg gctgcgtcat gttcgagcgc agtgttgatc ttgctggtga gcgaactgta
    45721 tggttcaaag tccacgtgcg ggttttcatc cgcagatgga gcgtagcgct tgatcagcac
    45781 ggctttgccg gcgacatctt tgcctgcgta atcgtcgtaa ttgccgtcgg cctcttcgac
    45841 cgtgatgccg tagccgacgt ccacaatggt gtcgaagtcg aacgcgaggc tggccgactg
    45901 cttcatcggc tcaaactccc cgtggagcgc caggggcgcg ccgttgagga cgaggcggtt
    45961 gcccggacca agctcgattg ccctgacgaa ctcgaaaggc tgcagccagg aaccgtctgt
    46021 cccgcgcggc tggagcccgg ccgcggcgaa ggcggactcg atgtagcggg cggctttcat
    46081 ttccccctcc tcgccgaccc ggcgcccctc gagggaatca tgggcaagaa ccatctcgtg
    46141 ggcccgcagg gaatccgcgg tgatcaggta agcggcccgg accgccggag cggccggcgc
    46201 cagcagcagc agcgcagcga tgagcatact cttcatacaa cttccttata cccggcaagc
    46261 gccaaaacct cgcactcccg gcgggcggca tcaacccgcc gtcggccgcc gacagagaac
    46321 aggggggaca atatcgcttg ccaatatacg aattgtcacg cgcttgtcac ggcccttgcc
    46381 gaaccgcggg cactttttga ttgccgcgcg gttaaggtgg attatattgc ccgctgaact
    46441 atacaaagga aagcaacggc atgacggaca gcatttgtgc agcttgcggc aaacccctca
    46501 cggccgggga ccgtttctgc gccgcctgcg gcgcgccgac cgaccttgac gttaagccgg
    46561 tccgcggcgg gaaacccgcc tcgaaaaagc ctcccgcggg cgcgccgcgc agcgcctcca
    46621 ccgggatgcg tgacggcgcg atcattgtcg gggcgcttgc gctgatcacg gtcggctggt
    46681 tcgtgtttcg gacccccgcc gttccgccgc agccgccctc cgccgcgacg ccggcgggcc
    46741 acccggaggt gccgggcgcc ccgatgggcg ggatgaccgg aggtctgagc gatctgccgg
    46801 ccgactacga cggactcgtg caggcgggga accggtacat ggacgccggg aactatgcgg
    46861 tggcggccga gagttatcgg cgggcgctgg ccctcgatcc gtcgtcgccc gacgtgcgca
    46921 ccgatttcgg cgcttgcctc cacggcatgg gactgcctga gcgggccatt gaggagttcc
    46981 gtgaggtgat gcagacccac cccgaacacg gcatcgccaa tttcaacatc ggcatcgtct
    47041 atctcgacat gggcaagccg gactcggccc gcttctactg ggaaaagtat ctccagcttg
    47101 agccgaccgg ggcggccgcc gccaaagcgc gcgaactgct cggcaacctg aatggatagc
    47161 gcagcgactt ttttgggcaa ctgggccgag gcgacctggg caatggtggt cgattcggcc
    47221 ttgctgcttc tgctcagttt cgtcctggcc gggctgctcc acctcgtgct caatgcccgc
    47281 acaatcggga aactggtctc cggcggacgg cggcgcgaag tgttcgccac cgcgctggtc
    47341 ggcgtgccgc tgcctttgtg ctcctgttcg gtgctgccgg tggcctatca actccggcgg
    47401 gccggcgtga gcaaagccgg caccaccgcc tttctcatcg ccacgcccga atcgggcgtc
    47461 gattccgtcc tgctcacctg gagcctcatg gatcccctca tgacggtcgc gcggccggtc
    47521 gcggcttttc tgacggcgtt caccgcgggc aacatcgagg cggcctttcc ggatgaccgg
    47581 ccgaccggga aagacggagc ggcagccggg ggagacggct gcgcctgcgg ctgcgcagcg
    47641 gagccgaacg cagccgtcgc gccggtacgg atcgccgatc gcgccccgcg cgcgtcctgg
    47701 ctgagccggg tgctggccgg gatcaggtac gggttcacgg atttgttcag cgatctggcc
    47761 gtctatctcc ttgtcgggta tctgctggcg ggtctggtgg cggccgtatt cggcagcgcg
    47821 ctgttgtctc tgccgccgtg gctgaccgcc ggctggggcg gatacgccgg ggcgatcgtg
    47881 atcggcctgc cgctctacat ctgcgcgacc tcgtcgacac ccctggccgc ggcgctcctg
    47941 ggagccgggt tttcgccggg cgcggtgctg gtcttcctgc tggtggggcc ggccaccaat
    48001 gtggcctcga tgaccgtggt cggcaaaatt ctcaaggggt ggggactggc gcggtacctc
    48061 gggtcgattg tgattgtggc cgtgctctgc gggatcgcgc tcgactgggc ctaccgcacc
    48121 gtgggtatcg cggccgcgtt ccgcgccggc gggcatgacc atgcctcctg gtaccacgtc
    48181 gccgccggcc tgctgtttgt gctgctgctg gcgcgggtgc tggcgatgaa gatccggcgg
    48241 cgcctgcggc gcggacggat gacaccggcg gccggatgag ctcgggcgcg gcgcgggcgc
    48301 aggcgggagc ccttgccgcc ggcgtgaccg caaggtatct tggaggcatg gcggatcaga
    48361 ccttcccctg cgtgtcgctg atcatcccga cctacggtcg cgaagaaccg ctggtggcct
    48421 gtctgaggtg cgcgctgaat cagtcctacc gcccgctgga aattctcgtc atcgatcaga
    48481 ccggggagca cgatccggag acggtccggt ttctccaggg cgcggccgac cgggtgcggg
    48541 tcattcggca cgcaccgccc agcgcggtca cggcgcgcaa ctgcgggctg cacgaggcgc
    48601 ggggcgagat cctcatcttc attgacgacg acacgacgtt cgggtcggga tttgccgcgg
    48661 cccacgtcgc cgcgcaccgg cgcggggccg aggtcgtcca gggacgggtg gtggagccgg
    48721 ggcgcggcgt ggcgcaccgg ccccagtggg tgcggccgtg gctgcgattt gtgggcagca
    48781 acacgcacga ccgggcgggc gtcaccaaca ccctcaccgg ctgcaatttc tcgctctcgc
    48841 gccgagcggc tgaccgggtg ggggagttcg atcccttatt ctgcggcgcc ctggtccgcg
    48901 aggatgccga ctacggggcg cgctgccgcc gcgccgggct gcgcatggtt ttcgacgccg
    48961 atgcctgcct gacccaccac cgggcggcgg gcggagtgga ccgcggcgtg accggacggg
    49021 agcgcgaatt cgatctctcg ctgctgcgca atgaactcta tttcgcccgc aagcatttcg
    49081 cgccgccggc ggtctggctg tacaagctgc gcatggcccg gcggctgcgg cggattgccc
    49141 ggcggtccgg cggggttccg ccgcacccga taagaaagct catccgccag gccgacggcc
    49201 aggcgcgatt gatggtccgg gaaggcgaat tgcctgaact atccgacgcc ccgcgatgat
    49261 ggggaaggcg ttttttgctt gagccgccgg gccggggaga tgagaattcc gtatgcgctg
    49321 gtcggcccta atcgaaaatc cggtcacccg gagcgtgctc gacttcctct tcccgccgct
    49381 gtgcctggga tgcggcgtgt tctacgatgg cgacaacctg gtgtgcgggc gctgcgaggc
    49441 cggcctggcg gcgctggcgt tcgagggaac catctgcgcc ggatgcggag cggtgatgtc
    49501 cgccagcgga cggtgccccg agtgcgggcc ggcgttctgg ccgctgttcg cgttcggcga
    49561 ctaccgcccc ccgctgaaag aaatcatcct ccagtttaag ttcaaaggga tcgtcgctcc
    49621 cgcggactgg atcgcggcga gactggcccc ggtggtcgac
//
LOCUS       DAOPOE010000012        48652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_938_length_48652_cov_5.698027, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000012 DAOPOE010000000
VERSION     DAOPOE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 48652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48652
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_48652_cov_5.698027"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKM94_03055"
     CDS             <1..373
                     /locus_tag="PKM94_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOD65645.1"
                     /translation="GNDLERATKLARRMVCNWGMSERVGPVTFGRTEDHIFLGREMAQ
                     TKDYSEATAELIDQEVRRFIETAEEIARGILTAHIDKLHALSHRLLEKEAVDSREVDE
                     IIGRAPSAAAEVESPTPTPER"
     gene            434..994
                     /gene="folE"
                     /locus_tag="PKM94_03060"
     CDS             434..994
                     /gene="folE"
                     /locus_tag="PKM94_03060"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOD65646.1"
                     /translation="MDHEKIMAGVQLILEGIGEDPNREGLVRTPERMAEYFEEVLAGY
                     AQDPTQELRKYTTSNKDEMIIIKDVSFYSLCEHHLLPFFGLVHIAYIPQNDKVAGFSN
                     MVRVVEILAQRLQVQERMTTDIADAMQAAIEPKGVLVVAEAEHLCLTMRGIKKPGSRI
                     VTSAVRGMMRRPATRNEALTLMGRGE"
     gene            complement(1022..1717)
                     /locus_tag="PKM94_03065"
     CDS             complement(1022..1717)
                     /locus_tag="PKM94_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65647.1"
                     /translation="MQRYLKRETELFVDGLSAGTAAACLEPLQFVLGGGGPGTGARPG
                     ASDFRGLTIHRTALTAAEVPVLRDGFIIPASLEICAPLQDALLAGEPVANLALSAAQL
                     VAYASDSEDCIRALGARYAAADTLRTARLIVAEKQPLSLPESTLQSYVGVYEIAPGSA
                     IEIILGADGLVAINPYGDRLKLYAEAAGAFFVRYPLAEITLSFVLSEDGSVSHAVLRM
                     GDSEVQARRRADN"
     gene            complement(1733..2677)
                     /locus_tag="PKM94_03070"
     CDS             complement(1733..2677)
                     /locus_tag="PKM94_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD65648.1"
                     /translation="MKPLCALVLLAALGVSAGAAPDPADSRPSDSVLTVAVLGSSVAA
                     GWVTSFQTRYDMENGWAARLGRALDPRRYRLVNLSVPGDNTAKALARFDGDVLPADPD
                     FLIIALSLSNEGLETLDPDSVCAAFETGLLSLVARCRDRGIQPILASCYPNSGFSEAQ
                     YAYLQRTNLRLYAWNLPGLNLLGALDDGRGRLLDGYTYDEGHPDDLGHEELSLSIVPT
                     IFDALREGKEAVTEFSGERYVTVGTADSPLPLSWVPSHPVHSFTFAFQVRTDGPGAIA
                     AITSRTHAASLAIDGDGRVIWSGGDGAAIVSAGAAPGR"
     gene            complement(2822..3181)
                     /locus_tag="PKM94_03075"
     CDS             complement(2822..3181)
                     /locus_tag="PKM94_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOD65649.1"
                     /translation="MRMKLKRTYEPPSPGDGYRVLVDRLWPRGLVRQDARIDRWLKEI
                     APSPALRTWFGHDPEKWADFRRRYFTELAANPAVGELREILHTRRTVTLVYAARDKDH
                     NHAVALKMFMERASARS"
     gene            3310..4194
                     /gene="folP"
                     /locus_tag="PKM94_03080"
     CDS             3310..4194
                     /gene="folP"
                     /locus_tag="PKM94_03080"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD65650.1"
                     /translation="MSRETLTHDGTSGRAIRLADGRRLSLARPLIMGTLNVTPDSFSD
                     GGRHTSRSMAIGRALEMEGEGADIIDVGGESTRPGAAPVDVDEEMRRVLPVIEGIRKA
                     SDIPISIDTYKAITAKAALDAGADIINDVSGLRFDEEMAGLVAARGVPVVIMHMKGTP
                     RDMQKNPRYDDCLAEITAFFRERIVHAERQGIAPAQIIIDPGIGFGKRLEDNLEILAN
                     LGRFRDLGFPLLVGPSRKRFIAMIHPSSKPADQRLGGSIAAVVAAALGGADIVRVHDI
                     DQTMEALRVAQALRGAVR"
     gene            4191..4952
                     /gene="cdaA"
                     /locus_tag="PKM94_03085"
     CDS             4191..4952
                     /gene="cdaA"
                     /locus_tag="PKM94_03085"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOD65651.1"
                     /translation="MSLFSWGFVSFGLKDLIDVLIVSFIIYEALKLMKGTRSAQIIVG
                     LFLVAGVAFIAYWFQLEGLTWLFSNLATFGLIVLVVVFQPELRGVLAQLGQRRFFRRF
                     VQLERKKSLEEVSRAALRLSELRYGGLVVIERRTGLRNFAESGKLVNAELSSELLVTL
                     FTPYTPLHDGAVIISGEYIVAASASLPLTTNPRYRKLYGMRHKAAIGVSEISDAVVVV
                     VSEETTGISIAYNGGLEKDIKKSEFRDKLASYLAR"
     gene            5125..5679
                     /locus_tag="PKM94_03090"
     CDS             5125..5679
                     /locus_tag="PKM94_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65652.1"
                     /translation="MAGKSESIGSFLLKALILLVVIAAGVFAGFWISSKTGLTGVFGG
                     NAAPQEMINRSNVRVGDIFPPVTVRRDGAETDIRTLLLGKRSLVAFVSGACEPCRLLV
                     EEAARWPSVIEGNLQVLLLTRTPEAHRGSPLLQVYEIPAEVLPQLEIGVFPTLYVINE
                     AGEVRVALSGYSEAMTEEVLTGRL"
     gene            5772..7577
                     /gene="lepA"
                     /locus_tag="PKM94_03095"
     CDS             5772..7577
                     /gene="lepA"
                     /locus_tag="PKM94_03095"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOD65653.1"
                     /translation="MTTPLKNIRNFSIIAHIDHGKSTLADRLLQVTKTVSERQMREQV
                     LDSMDLERERGITIKAHAIRLEYRAADGAVYQLNLIDTPGHVDFTYEVSRSLAACEGA
                     VLVVDASQGIEAQTLSNLYLAMEHDLEILPVVNKIDLPNAETEKVVAELVDLLGVPRE
                     EVLRVSAKHGTGITEVLEAIVRRIPGPKGDPGAPLQAMVFDSIYDSYRGAMPLLRLFN
                     GTLRTHDRIRAMATGKTYDVDEVGYLRLGTAPAEALVAGEVGYLFASIREVADTKIGD
                     TLTTVENPAAAPLPGFVNIKPMVFSGLYPAVAEDFTRLREALEKLALNDSSLSYTPES
                     SQALGFGFRVGFLGLLHMEIVTERLSREYGQLIINTVPNVEYHVYRTDGTMAAVDNPA
                     DMPPPQETDHVQEPYVDAQIIVPSEYVGAIMKLATERRGEYKNTEYLSAARANLSYAF
                     PLSEIIFDFYDKLKSVSRGYASFDYGVPYYRRSDLVKLDILVNGEPVDALSVIIHRDR
                     AYSWGKSLNEKLRELIPRQMYEVVIQAAIGSRIISRATVKPMRKNVTAKCYGGDITRK
                     RKLLERQKEGKKRMKQFGRVEIPQEAFLAALQIER"
     gene            complement(7605..8900)
                     /locus_tag="PKM94_03100"
     CDS             complement(7605..8900)
                     /locus_tag="PKM94_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOD65654.1"
                     /translation="MIKTSFAFLLIGTVLLAVQCSDPVEPTSPDDPTPVRTPAQLTGP
                     ETDLVQSVNRFGFKLFRLVNENAAAGENIFVSPLSVSYALGLALNGAEGLTRDEMAAT
                     LELAGSSPDETNEAYRGLTEILSGADPAVTFTVANSFWSRLGLRVQPSFTDMGREYFD
                     ARVEELDFNAPWAADTINSWVAGATGGRITEIIESPISPEMGAFLINALCFKASWTYP
                     FNEDFTRELPFHPGGGATVLCPTMWKRTADEIEQDPGRFAARPMMYGEHELFQAATLP
                     YGRSGFRMTILLPHPWVAVDSLVVLLDPDTWRTWSSLPTVADFEILLPRYKFACEATL
                     NDILTRMGMRTAFDPEAADFRRLFADVGSYIGEVKHKTFVEVDEKGTEAAAVTSIGWG
                     VTSLPPLFRVDRPFLFIIHEAESGAVLFMGKVVNPARAG"
     gene            9075..10334
                     /locus_tag="PKM94_03105"
     CDS             9075..10334
                     /locus_tag="PKM94_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOD65655.1"
                     /translation="MKRLLCTLLFIGLPLPAAAARLLIPMDDSQTDHLKAYGVVFQGL
                     EKGSKGEWLLNYRGGSFLLDYNQDNVNRCLLMGVAAEQVSEGQVADIYRQIEAGNMER
                     VELEKAPSIAVYSPPSAQPWDDAVTLALTYAEIKYAIVWDEEVLAGALDDYDWLHCHH
                     EDFTGQYGKFYAAFRNELWYQADVKTNEDMARRLGYMKVSDMKLEVAKAIYDYVRRGG
                     FLFSMCSAPETIDIALAAEGVDISPSEFDGDPVDPNCQARLDYSKTFAFENFTVELNP
                     LEYRHSDIDTYPDRQIRFLSPDEDYFFLFDFAAKLDPVPTMLVQDHTATVAGFMGQTT
                     GFRKSLLKDFVTILGQPDNYDEVRYIHGNLGRGTFTFLSGHDPEDYRHMVHDPATDLA
                     LYKNSPGYRLILNNVLFPAAKRKERKT"
     gene            10339..10776
                     /gene="ruvX"
                     /locus_tag="PKM94_03110"
     CDS             10339..10776
                     /gene="ruvX"
                     /locus_tag="PKM94_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOD65656.1"
                     /translation="MAAGGDTRTLLGIDYGARRIGVAKSDPTGLIATALTTLVVKSAA
                     DALAQVAALIREHQPAGIVIGSPVGLSGESNRKCLEIEAFANALRTVFDGPIHLWDEG
                     YSSVEAADIIHAHGKRVGKDKKRIDRLAAVIILQRFLDEQPER"
     gene            10847..11833
                     /gene="mltG"
                     /locus_tag="PKM94_03115"
     CDS             10847..11833
                     /gene="mltG"
                     /locus_tag="PKM94_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOD65657.1"
                     /translation="MGRRIVTAAAVLLAAAIIGLAVGQHYRTVDLGDRVATVIIAPGD
                     SFAQVAARLDAQGVVPSPRLLRLAARLTGVDRRLIPGRYDFTGKQSAAGVLDRLARGD
                     LLRIRVTIPEGTTLWRTAGLLARSLELDSAAIVGLGSDSAFLAELGKPCLEGYLFPET
                     YFFPWGIDEREAVRTAVAQFDRQTEGLWAGERTLGLSPHEIVILASIIESETPLAGER
                     GLVASVYANRLRQKMRLDADPTVIYGLGGLDRPLSRRDLRKDTPYNTYLHAGLPPTPI
                     NSPGLASIKAALAPDTSDYLFFVADNAGGHFFSKTNAEHERARRRIRQERGR"
     gene            complement(11853..12395)
                     /locus_tag="PKM94_03120"
     CDS             complement(11853..12395)
                     /locus_tag="PKM94_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD65658.1"
                     /translation="MTDKPLPRKRSYLRTPAPTRPIPVDFAVLHEQGAGAPQMFAHLT
                     RELARRYAINRGVLIMRRGAAGPFAAVSTWVNGSLRDGLAVNLPPQSSLFEKVAEHGR
                     VYTENFCASFSGNFFERKLLLGDESRSFVVQPLTSDGEVVGLVAYSSDEPTAFAMFEE
                     GAVEDVARAFAAAIRKQRLA"
     gene            complement(12385..13755)
                     /locus_tag="PKM94_03125"
     CDS             complement(12385..13755)
                     /locus_tag="PKM94_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD65659.1"
                     /translation="MITVRPAAARILVLDPGQTERRSLSLPLADNTLEITEVNTLDSA
                     LAVLATGQIHLVLVRHDAALPDPQELIRRVRRLDPAVEILLVLPAAPPGRIAAALEAG
                     AFDCVAEPPSTEALLARARKAIEHRELKVRLAAVRQHVAMHYSFDNLVGVSRAAQQLR
                     EALARLAPTDIAVLITGPEGSGKELCASILHHHSGRRQRPYVVVDCTAPEPVLVPELF
                     GSEGSDRPGLLVQADGGTVLLRRLDRMPAAVQERLAEVLKTGVLPGRPARLNVRWLST
                     SALSAGELAAAGVRRDLLAKLDTITVAVPPLRERIEDLEPLVAAMLRRIAAETGRTRL
                     DISRAALDRLLHWPWPGNARELESCLRRAAALAGGETIDSDAVVFAEHRPGAAEERPP
                     APRTNSLRLADNQRDLIEKVLIDNNWNYTQTAQELGIGRTTLWRKVKKYNLKPGVAGE
                     AVRDDG"
     gene            complement(13752..15143)
                     /locus_tag="PKM94_03130"
     CDS             complement(13752..15143)
                     /locus_tag="PKM94_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65660.1"
                     /translation="MTTTATTSKQSRLERLDRFESKRFPTAVSEESAVVRAASLLLGV
                     EIRGWLSRSEQEYLDSLAPDNALTFLVRGDRYLHPEEVPLFPPDLIDAAVRQGRDAVF
                     SHRCAAGAASGPGISRVFTGVIEDRNGAPLVLGMLCPDSLTDKVVLERCFERVISAFR
                     SARDSVRGMLAALGPTPPGAARILVNRASGRVFHADPDICTAAACEPSALVAREYGTV
                     EPLVRRLVARFKMKLENHACGCLHVACLTFEPAREAAAETRLSLACVDSLRRDAAGLT
                     TAAEYIAGNSGLLAAGEIQALASEIARTGRSLSRRLARTSLVARYGQMEPARVNLAHQ
                     IDHAIDRFAGLDRERISLVLDGGGEQFLVSAPPEALLVLFETVLEAHAAADRAAATAV
                     RVDCADGAPEARVVFTTIVSDAARIADLERAWPADADRLVRRLGAAVERRVDAPSRSL
                     TTAVTFRTQEIPA"
     gene            15545..16273
                     /locus_tag="PKM94_03135"
     CDS             15545..16273
                     /locus_tag="PKM94_03135"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOD65661.1"
                     /translation="MAGVVAIIPARLGSTRFPRKVLHLYRGRPLLYYVWREVSRARRI
                     DRVAVATDSREIVAAVREFGGEAVRTSARHRTGSDRAAEAAEKIGGRIIVNVQADNFG
                     LTGAALDRVVARMEQDRTIEFATLAFPIGGAEDLADRNLVKVVASDREGRALWFSRWP
                     IPLVRDAGARYRHLGHIGVYFFRRAALRRYAEWPRTAAERAESLEQLRILEHGGRMLV
                     FETRARTVSVDAPPDVAKLDTLYR"
     gene            16282..17958
                     /locus_tag="PKM94_03140"
     CDS             16282..17958
                     /locus_tag="PKM94_03140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOD65662.1"
                     /translation="MADKTKYIFVTGGVVSALGKGISSASIGLLLQRRGLTVYNMKLD
                     PYLNVDPGTMNPYQHGEVFVLDDGSETDLDLGHYERFLDRSLTRDHNVTTGQIYHTII
                     TRERRGDYLGATVQVIPHVTEEIKNRIRRLERLPEKPDVVICEIGGTVGDIESLPFME
                     AIRQMGLEQGYDNVMYVHVTLVPYIATAGEFKTKPTQHSVKELRAIGIQPNMLLCRSA
                     KPLDEGLRQKISLFCSVPTKSVIAAEDVSTIYEMPLRFRAQGMDDIICRHFGLETPEP
                     DLGDWEQMVERISYPTHRLKIAICGKYVMLKDAYKSIIEAFVHAGVHNGAEVKLIWVS
                     SEEIKHNGAGKFLHDVDGLLIPGGFGERGVEGKIEAIRYVRENRIPFLGICLGMQCAV
                     IEFARNVCGLEDAHSYEFYRDLKHPVIHLMADQEGVTELGGTMRLGAYPCRLAAGSLA
                     RQAYGADEISERHRHRYELNNAYREMLGSHGLRLSGMSPDGRLVEIIEHPEHPWFVAS
                     QFHPELKSRPTRPHPLFRDFVKAAVDYKVRRNGASGSNNEEEHDLSEAAR"
     gene            17987..18475
                     /locus_tag="PKM94_03145"
     CDS             17987..18475
                     /locus_tag="PKM94_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65663.1"
                     /translation="MSRAGKARFRPLILLLAAAALAGGCKEDRQALAPPEVYTGMMVM
                     EPDFASGPMEIYPDSTMVTFTVEGTLYELLLEDETDFHLCHTRGTVRSFGTNALTLAP
                     TGTFGAGNCDSIRVPRGELPTVFRGDSLFIGPKDIAYEIHINNTTVTDIMRYTFRLRR
                     VQ"
     gene            complement(18502..19614)
                     /locus_tag="PKM94_03150"
     CDS             complement(18502..19614)
                     /locus_tag="PKM94_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65664.1"
                     /translation="MNFARLRSKSFLVPTAVTFLFLAVVLALLGTGERKYAPLARTLI
                     QWDGRHYLSIAETGYEKFPCPEDPSLVCGNVGWFPLFPLAARVVSVALGPLGLGMAWS
                     MLITGWLCLWAAMLLLYRLFARRFDHTVAAGSLAALALFPTSFYYLTAFPYALYLLLA
                     AAVFLLVEKERYFAAAAPAGLLAITYPTGVAIILPLLWVLVSRWRGLTPRDRAGLRTA
                     SAAVVLALILYALYYRLAFGDFFLYVHFQSKPYYAHGATVPLLPILDAVVHLPGAHPV
                     RVMLIFVAAALALFSTRRIPAAWQVFLWGVLLFTPAAGTTACYYRHVVVAFPLFAMVG
                     LSLRSRRRPLAVLYAAAALALMLGVFLDAYKRGVLM"
     gene            19752..21473
                     /locus_tag="PKM94_03155"
     CDS             19752..21473
                     /locus_tag="PKM94_03155"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOD65665.1"
                     /translation="MDLRKPKVATVGLSAVLALYYALPFLLKLTWWGVRDWDLFTTIA
                     AVPVGTVLHYGQFPFWNPYLGGGNILFHHPEVAVLSPFVLLYFLCGPVVGLKLQVLIA
                     YFLGFWGSLRLFRTLGMPRGSTVVAAVAYFGSVHFALHFAEGHMPFTHYAFLPWFVHF
                     VLKGAEDRRNLLGAVVALALMILGNGAAVPLLYTLLATALLLALRAAQRRSPAEARVF
                     LLAVGLALALAAVKVLPMIAYLAQNRWTGDPAEAIPWSALPAMFFGLKHSLFVQNFAG
                     QKWAWHEYGVYVSPLLVAAGAYAAVARFRRHWPWVVFIAFFLLLGLGNFAPWSPWALL
                     TGLPGFESLRATGRAMHFVILGAAVLGGLGLGCWEDRARAAARGGWLRGAVVAAGLLI
                     AATNLALAWPIMSEGFRKPPKHVDRSPEFRQVVDAEPHAFENYLANRGSLVSPWLSAY
                     HPSRGLVGPGIVYSELFSGGEAKVYVRDYTPNRITYTLNGGGHGGELVIGMGYDPGWR
                     ATDGRTITENQGLIAFPVTPGRQTVVLVYRTPYFSHGAVISLLTLGGMVLWRRRLFGR
                     PGRGANL"
     gene            21576..22901
                     /locus_tag="PKM94_03160"
     CDS             21576..22901
                     /locus_tag="PKM94_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOD65666.1"
                     /translation="MRLVSTILAVLMVTAAAAIAQPDRADSVGAGGYRFFDMPIDPER
                     YLLRPDERLQVTFLGTALAPLTVRVNPEGRIVHPTLGSFDLRGATLAEARVALVKALS
                     SLYQADSIDISVLAPRRVAIQVSGAVQSPGLYTVYTSQRVSEVIDSAGGLSPSGSRRL
                     IRLLGGPREIRVDLDRALLGDERWNPPLYAGYTVIVPAKSARVVNVVGAVDRPRDIEI
                     VDGESVAEILPLVGPFRSSADTLAVQIIRGSERLPAAGVPLQAEDIVFVPTLSRLAEG
                     APVTVYGAVARPGRFLLSASGALSEILDAAGGFAPDAARSELTVFRQVPLEAALRAGE
                     RYSIANLIALDGAVQPFTLQPGDSIFVPMMVGYVKVSGEVRNPGMFPFRVGGTARDYV
                     RAAGGFLPRADREMVEIFHRVSRLTEEHPAEVVVHDGDEVIVRLREEFR"
     gene            22898..24106
                     /locus_tag="PKM94_03165"
     CDS             22898..24106
                     /locus_tag="PKM94_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD65667.1"
                     /translation="MNDRAAANNFWLFLELLARRRTFIVTFVLLATLAAVITAFALPK
                     WYRATALLLPPKDVTLPTPGAGRLEDVVSVTKGLNLPVMVTPSDVYARMLKSRTIARE
                     IVERFDLMALYKEDNFVETYDVLMDRARFRVTEEGLLEISVEDRNAARAAAIANAFVD
                     VLDSINREIVTGRVRQNRTFVQERLAVAKVELDSARAQFREFQMRNRAIDFDEQTRLA
                     IDQAARLKSDLASLDIDIAMKEQTLGADNTDLRELKRRRATVQDRLDQLERSNPDSSF
                     FSLPVSAIPTLRGQYEVLYSRVRVAESIYRILLEQLEEVKYQEQEKQPVLSVLDRAVP
                     PEIKSRPQRSIIVLAAFALAVLAAVLLAAAVDYVDRMRTASPENYRRVQMFAQAWFGW
                     LPGLKRRPAE"
     gene            24178..24939
                     /locus_tag="PKM94_03170"
     CDS             24178..24939
                     /locus_tag="PKM94_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOD65668.1"
                     /translation="MTSSEISPEARIDPTAHIGRFCVIGAQATVGAGCVIGHHVVIHE
                     DTAVGDNVRIDDGAVLGKRPMRAANSAVTREQDLPPCTIAAGCIIGTHVVIYRGARLG
                     AQVLVADLATIRENVTVGRRTIVGRGAAIENQCEIGAYCKLETNVYITAYSTLGDRVF
                     VAPCAATSNDNFVGRTMERFKRFKGVTVKTGGRIGVHATILPGKTVGADALVGAGALV
                     TADVPDGKIVVGMPAKVFRDVPEEQLLKNQGWKDE"
     gene            24952..26058
                     /locus_tag="PKM94_03175"
     CDS             24952..26058
                     /locus_tag="PKM94_03175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD65669.1"
                     /translation="MAVPLLDVKRQYQALKAEMDEAVLRVLAQGQFILGPEVAQLEKA
                     VAALSGVRFGIGVASGTDALLLALRACGVGPGDEVITTDFSFFATAGVVARLGARPVF
                     VDIDPDTYNIDPDLIAAAVTPRTKAIMPVHLFGQPADMDPICALARRHGLAVIEDAAQ
                     SIGAAYKGRPAGSFGDYGCYSFYPSKNLGAIGDGGMIVTDRPENDQRVRILRVHGAEP
                     KYFHKLVGYNSRLATIQAAALLVKLPRLEAWSKERAAHARIYDEAFAGIEAIRRPAVK
                     EYTTFHIFNQYTIAVPNRDRVMEQLKAAGIGCEIYYPVPFHKQECFAYLGYRPGDFPV
                     SSKAADEVLSLPIYSEMTEAEQQEVIAAVRRILG"
     gene            26147..27703
                     /locus_tag="PKM94_03180"
     CDS             26147..27703
                     /locus_tag="PKM94_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOD65670.1"
                     /translation="MTGGSMQRAVFGVAGVLLLSRVFGFVREMVIAHRFGTSADYDLY
                     LIAIMFSALAYGILNYAGYYLFVPYLTRRLQARAGAEGPPDFWPLVNTGAAAALAVTL
                     LLAAAGPALLRLWAVDYVGEGFDRIAFYSRVTAVVVVLGVAEAFMRAFLNVKRVYTHP
                     AAGYIVFNLFSILSIVLLAGRLGVGAIALGWIGGLLAQNVWLAARLVRVHAFGGYRPQ
                     MITEETKYLIATASALIVIELINRSYFLIDRYFAPQFGPGVISALNYSQVIVQLPDSI
                     IGWALGAVVFPMFSAHRGEADRAKFWFLYRRTVTAALVIAVPIAAALFITAPEVIYVL
                     LQRGEFDPQSTATTAAVLRPYVPSLIALFIVSTSIRACYSGGWVRAVFWATVSVFAVK
                     AAATWGLSALFGPGGISAATSAAEVVFAVLLLGIAVRRIAGEGRAAFLGVLGRILAAG
                     AAAAALAGLGQQLLPEWAAAGGRGPALIKIVLSGLAVAALYVLFGRLLGLRRQFADVL
                     ALRTQRAGEA"
     gene            27707..28774
                     /locus_tag="PKM94_03185"
     CDS             27707..28774
                     /locus_tag="PKM94_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD65671.1"
                     /translation="MGARVVIFGWSGSVHVQRWVTGLQARGFEMKVVSLGGPPLADCE
                     TTVLPRTGRWSYLTHAARAAEAARAFAPDLVHAHYATGFGYWAVRSRLRPSLVSVWGA
                     DVIDFPAGWFRARLLRAVLRRATHLSATSQFLRSKVIGLDGSLASQTSVIPFGVAIPA
                     EAAAPPPAPPVRLCYVKNLRPKYGADVLLQAMARARKELPDLRLDIAGEGEMKARLLE
                     KTKNLGLDDLVEFVGFVPNDRVYAFIREHHIMVMPSVCLESFGVAALEAAACGRPVIA
                     SAVGGVPEVVRREETGILVPPGDSEKLAEAIVRLGRDAALRERFGRNGWAFAKENYNW
                     ELSLDRMSELYLRLIDEKKKS"
     gene            28755..29927
                     /locus_tag="PKM94_03190"
     CDS             28755..29927
                     /locus_tag="PKM94_03190"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD65672.1"
                     /translation="MRKKRAERRVPLFDLAVSPAARRYVNDALASGWLTAGPKVKAFE
                     AAVARLTGVRHAAAVASGTQGLHLALAACGGVPGREVVTTPYTFVATVEAILLAGGTP
                     VFADIDPHTLTIDPDAAARKVGKKTAAVVPVDIAGHPCDYPRLNDLCGAHNLPLVADA
                     AHAIGGAYRRRAIPKLCDAAVYSFYSTKNLTAGEGGMAVSRHKELIDRIRLLARHGLT
                     SSTYERKLSNRWEYDAVAFGWKANMAELSAAVGLGEIERFGANQRARAAAAARYRRHL
                     AALAEYVVLPTEAPDCTHSWHLYIIKLRTEALRIGRDRFIREMAARGVECGVHYKPVY
                     ELSWYRRAFDLSARPFPNAEWAWRRAVTLPLYPTLAAADVDYVCACIAEIAAQFRR"
     gene            30015..31013
                     /locus_tag="PKM94_03195"
     CDS             30015..31013
                     /locus_tag="PKM94_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOD65673.1"
                     /translation="MPANLPPQYYELERAYNAEKDPREKLRLAQELLRIMPKHKGTDK
                     LQAEMKAKISRHRKEIEAGGARAGGAAKAVAKDYIEKEGAGQVILIGPPNAGKSSLLD
                     ALTSAKPEIGDYPYTTREPQPGMMTFETVQIQLIDTPPISVELYENYLTGLIRNADLV
                     VLVCDLEARSMLDDLRFIIATLREKRIILQPEVAGEPDDPKYCGKRTVLCAHKEYEDE
                     DGSKRRQLAGAYPGYRIVTTSIIDEASLEAFRRAIFEELRIIRVYTKPVGHDPDYRDP
                     IILPAGGTVAEAAATLHKDFLQKLKFAKVWGKGKFDGQRVQNDYALSDGDIVEFHI"
     gene            31126..31743
                     /locus_tag="PKM94_03200"
     CDS             31126..31743
                     /locus_tag="PKM94_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD65674.1"
                     /translation="MKVLGIALAVILILVLAVAGWIWSGRGGLIDRSQAIDEKWAQVQ
                     NVYQRRFDLVPNLVASVDNFMERQQATLTEVIAMRQRVIDLTASAESALGSGNPALLD
                     SLAGQLSRQLNSFINVVVERYPEIKGDQLYADLMTQLEGTENRIAQERRVYNEAVREY
                     NVYRQRGVKALIAGAVFGFPYAREFFAAQSEAQTAPSVRDAFEKK"
     gene            31758..32477
                     /locus_tag="PKM94_03205"
     CDS             31758..32477
                     /locus_tag="PKM94_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOD65675.1"
                     /translation="MKKLIPIAAALAASALAAAATLPSYTGPINDQAGILDNRAVEQL
                     EAKVLAYRDASGHEIGVLIVPSLEGRGIEDYAHDVFRAWGIGKKGQDNGVLFLAAIED
                     KRARVEVGYGLEEALTDLEAGRLVSRNSPMAQQFRQGDYAGGVAAVVDGIIEAIGGEY
                     APPESDESPAAAIFPLLVMGLILIVALANAASRRGISGRRFGGPFLGGFGGGFGGGGG
                     GGGGFSFGGGSSGGGGASGGW"
     gene            32492..33124
                     /locus_tag="PKM94_03210"
     CDS             32492..33124
                     /locus_tag="PKM94_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65676.1"
                     /translation="MASLVEKYFSAADFAAIEAAVRQAESATSGEIAVELASRSRNWS
                     GERLLCALAVTFVCMVAALLATRRVDWGVYYNTTQTLLWGGVGFVLAYFGGGRVLART
                     ARRRAAVWRRALSAFHQMVPVRGRTGVLIFVSVEEGQAAVIADRGIAAKAPPDYWGGP
                     CGTIVAGMAKGEHAEGIIRAIAAIGEELARHFPRMDDDVNELPDRPKVDE"
     gene            33197..34330
                     /locus_tag="PKM94_03215"
     CDS             33197..34330
                     /locus_tag="PKM94_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65677.1"
                     /translation="MDQPLYYDFRDILIAPARALAAKRIFVMTLFLCLAVAIYDVFVY
                     LAAVLDGASLETFWRWHGLLPLAGLNFSGGLAQAACGLGIVLALLALMTGLFAVSAIE
                     IEQLRGNRFFPMCEAIRFARRRLGQLAAGEAAMVAFLLFILLLFLLLGLVVRIPAVGE
                     WVYAATFLLPVFFIAIFTVFIVAVLQISVILLPAAAAAERHGESFTAILGTFSTVIRQ
                     PVRWLAYTAYGLAAAKAASWIYAYFCYRAVQFTAWAASLTAGEKADRLVREGLGHLPV
                     RSGPVEAAVALVPGFDWRFSISQWAERGDSAAGYVMAAMLLVILASVFGYALAVVATT
                     QARAYAVIRFLKDDYRIAEEPPLFLPEAREDETGSGGAGAPAG"
     gene            34463..35377
                     /locus_tag="PKM94_03220"
     CDS             34463..35377
                     /locus_tag="PKM94_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HOD65678.1"
                     /translation="MTAEKKILVTGVTGAQGGSVARHLLRRGRFAVRGLTRHPESPQA
                     KDLARRGVEIAAGDMGDPQSLVRAMDGCWGVFGVTNFWEHFHREFELGRNLIRAVAET
                     RPEFFIYSSLPNPRKLGRGEYDVPHLELKARLEDEIRALPLRSAFTHVAFYYENYMIF
                     SMLQRAEDGSLSFGFPQGETKLAMVGTEDIGGVVAPLFERPDEFAGRQVGVVGDDRPP
                     AEYAQALARALEVPVSYRHIPREEYAQFPFPGAQELANMFDMNRRYITERTADLEESR
                     KLYPGIRDFDAWVRENREFFASLLEPAR"
     gene            35486..36016
                     /locus_tag="PKM94_03225"
     CDS             35486..36016
                     /locus_tag="PKM94_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOD65679.1"
                     /translation="MLIFATIFAVGFLTLLLSVIFGHDTDFHVDADAGGVGADGHGPG
                     VINIKIIALLLVGFGAVGFGFRATAGWTMMKSSMAGLVGAAAVGAIGYLILRLFYASQ
                     ASSTIGDEEIIGCEARLIDAIAGAEYGQAACVIRGREITFLARSADGRSIARNAPVRI
                     VGKSGGVVTVAPTQNG"
     gene            36051..37643
                     /locus_tag="PKM94_03230"
     CDS             36051..37643
                     /locus_tag="PKM94_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD65680.1"
                     /translation="MSEMIQFFVWPIIGLAIVIALFVLVKALSLFYVRVPPNKAAFFY
                     GARSGKKGLQRATQAEVPGVPGVTVLPPGTVVVTGGGRIRKPIIEDVQFLDLTEITLP
                     QIRVENMPNTDGVLVTVEAVANIRFKDDAQSLLAAGARFLSMPHEKVQSVARETLEAN
                     LRGVVGTLTVDQLIKDRDAFRQQVLREAAEDLARLGMQIDVFNPQSITDQQGYIEALG
                     KKRTAEVKRDAMIGEAEAQAEAKKRSTDAERAAEVVAQENERLKSEAQKNTNVAKQQY
                     AAEISKQTAITEQAGPLATAGERQKVIVAEVKIEEERSRAQIAVEEQNILREQKAQEA
                     EVVVPAKAKADSQVKEAEGYRQAQISRADGDKYATIARADAEKHKRSQEGLGQAEAVK
                     ATGLAEADVIERQGLARAVSVAELAKAYRQFNEAALTLEMLKILPQVIESCGAVFGAI
                     AAPMGNIDKLVVVDAGGGEGKDGALSRFAKTGPLLLFQLVEQAKAAGIDVSALVQRLG
                     IKLGETGEPGAAGAPAGGGATE"
     gene            37640..38254
                     /locus_tag="PKM94_03235"
     CDS             37640..38254
                     /locus_tag="PKM94_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65681.1"
                     /translation="MKRRRFLKNAALADDVISYRPAEPDPSDGPPGAPPREDGAPEEV
                     EADVIRSDGRPMYQLDTRAPEALVIHCGDPRFQTAFRRFVTEELGIRTYTPIVVGGGA
                     HAFGAQVFLPKNFKVLWEQVKFFTRQQNLRRVIIINHEDCLWYRSMKGYHPTVEVPVK
                     GKLDLRHAAQTIVRDFAGIHVESYWAALAEDRVYFVPVAPRAAE"
     gene            complement(38668..38796)
                     /locus_tag="PKM94_03240"
     CDS             complement(38668..38796)
                     /locus_tag="PKM94_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65682.1"
                     /translation="MSIPKPRPMILLTQGANSKTVVLMKSPAQPPRGGSLTLKWPA"
     gene            complement(38854..40266)
                     /locus_tag="PKM94_03245"
     CDS             complement(38854..40266)
                     /locus_tag="PKM94_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD65683.1"
                     /translation="MNTSRLTRAPLALPVPTLLLTLALLAVAAPRLTAAVTASAAGVN
                     SITLTWTAPGDDGVSGRAAQYDVRYSLSPITNATWASASQATGEPAPAAAGSAEIFTV
                     TGLNSGTTYYFAVKTADEALNWSALSNVASGTTAVAGDASSPAAVTNLIVVAPTTTSL
                     TLVWTAPGDDGAVGTASRYDIRYSTLPISNGVTWNMATQLVSEPSPSPAGAAETLTVA
                     GLAEATTYYFALKTADEVPNWSTISNCPSGITAADAAPPAAVVDLRASSGDADSTIDV
                     VWTATGDDGLLGTAAAYEVRYSRTAISESNWSAATLWPSVITPLASGLEQRVTLTGLQ
                     PGEVYYVALKAYDDAANVSPLSNVDTGVAKYGIASDVDPDETLPGGYVLDQNFPNPFN
                     PTTSIRFSVPAAGRAELTVYNTAGQEVTRLVDGALAAGEHTVTWDGRDRTGYPVASGV
                     YYYRLAAGDQQHTKKMVLLK"
     gene            40953..42401
                     /locus_tag="PKM94_03250"
     CDS             40953..42401
                     /locus_tag="PKM94_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOD65684.1"
                     /translation="MRKMMKVLVPVFMLALVVAGCSKGPVEPQTNMPLTASYALPIDA
                     ALESATLHIYVVGATNHPVTAHRVTAAWDEMTVTWNSFGAAFDAAVVDGFMTDAGWQT
                     TDVTSLVQAWMNGTYANYGILLDEADDQTPRSIYASSESADFPTEDPWIEFCYTIGAD
                     PTVYCDTIVITEDTYISEYDPNTNFGENILLFTGRPYLDGTPVEKQTLLYFELEVTPP
                     PLCAIGDFVWYDDNMNGIQDAGEMGVPGVTVQLMDCEGNVLDEMLTDANGYYLFDELQ
                     PGHYNIHFVLPAGYVWTLQDQGADDAVDSDVDANGTTICTELVAGEIDRTWDAGIYMP
                     EQEGCTRTPGYWKNWSGFGPQPDMVTQYLPIWLGTPMGAKSIHVTNAQTAVELLGRTW
                     GSSSNGILKLYCHLLAAKLNVAAGASADDLGTAIADADAFLAMYNWMDWDSLSKANKN
                     MVNRWVGLLDDYNNGCIGPGHCDNDPYAAPCD"
     gene            42899..46015
                     /locus_tag="PKM94_03255"
     CDS             42899..46015
                     /locus_tag="PKM94_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD65685.1"
                     /translation="MKRFVLGSVALLAAAAAVFAAQIIGPPDDQFDMSRITPGPLVAS
                     TFAADTAVNITLDPSSYPIIEMYVDVLDNSGYPIPGLTAGDFCVTQDGAPVPFQVTEV
                     GSQGCPTAICLVIDQSGSMASYGAMELAKNAARQFVRNMDAFDRVALVPFSSCTSVLQ
                     DFTSDTTLLLSRITSLIASGSTALFDGIWLGEDITAPQQGARAVISFTDGGENNSDVC
                     GYPPDGSYYDGTYADDSVIIVNKALAAGIPLYGITVGSGAWADPLIAFAGGSGGYYMH
                     APSAADIDSLYSRIKTTLCSRYLITFTSPDTVMDGTQHQVVVCQGGTLCTPCDTGWYN
                     EPCPPVIVRTQATIDLEAVCQPAATGLSVCALVADTCAPFLSNVTLFWRYANPAVGPY
                     NSAAMVSAGGNEYCYTIPGGLLPLGTSGIDYYVTASDGEATVSVPAANPQNYPYAVAI
                     CPNYAPEITGVAVDCEAGGDAVVAALITDTLDYVDHAVLFYRQAGDILYDSVLMANTG
                     GSNWEGVIPSEVTAGWFDYVVRAWDNFGVMAQYGPLTDSCGDGDEPVPTNEWINVYCG
                     YPLLNGVPLSPGDVIRAYDPDGVLCGMDVVRPDGSFGFMPIYREEPYNPGDQGAEPSD
                     LITFFINGVEAQADPEVYWTANGDGFEVCAFTTCQTFTLDAGWHLISWNREWSGSIED
                     FFALLGKPGPCIEVILGFDRGAQTYDPDLPEFSTLHGLDYHFGYWFLLNCPITFDICG
                     PRVDPTDFITMWPDWNLVSYWPTEELAPPDALASVYGCLMVAVGFDGGGLIHVPGDDP
                     YNTLATMGPGFGYWLKGECGGTLLYPGWTGPVPPLLPRTAAAAPEVPPSRTWLSLYGS
                     NLTLDGAALGDAVFEVFGASGVRCGVGRYSDGLLRFTPVYGYDATSATTVQYPKAGER
                     LTLTVNGERVATPLVWTDQGDRVRLSALTREAGGALPLSYGLAQNYPNPFNPTTAISF
                     TLAAAGPVKLKVINLLGQTVATLVDGPLAQGSHTVTWDASAMASGTYFYRLTAGAFTQ
                     TKKMVLLK"
     gene            complement(46085..47425)
                     /gene="mtaB"
                     /locus_tag="PKM94_03260"
     CDS             complement(46085..47425)
                     /gene="mtaB"
                     /locus_tag="PKM94_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOD65686.1"
                     /translation="MTEPVDAPRKTKVIRLQTVGCRLNQYETERMAAKLYPFGFRRAA
                     DGEPADLTVINTCTVTHRADADCRRLIRQAARRNPHGRIVVVGCYVDHDPEVIAGLEG
                     VDVIVPNRDKDAIDRILPARLPDLFREAPDLSCSPFIEDFHNRNRAWVKISDGCNQWC
                     SFCIVAIVRGRLANRPAADIAAEINALAAQGYREIVLTGVNIGYYRDRGREPQIRSLA
                     GLCRFLLAETDLARLRLSSVEPQAVKDDLIEVFAESRGRLCRHFHLPLQSASSEILCA
                     MRRPYNQAIYVQRASRLKAAVPGTTIGADVIVGFPGETEEHFEQTRRLAASGLIDYLH
                     VFSYSDRPGTAAAALEGKVPAAEVRRRNRILADLSRRLQRANLERQVGRTLEVIAEHR
                     SEPDGAYFALSDNYLRVKLPASAYRERALVPVAVTAAREDYLEGIPVGSPAPPA"
     gene            47580..48155
                     /locus_tag="PKM94_03265"
     CDS             47580..48155
                     /locus_tag="PKM94_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD65687.1"
                     /translation="MDKADKAPTAPVLALVGMPGSGKSTAAEMLAARGWATIRFGQVT
                     IDEIAARGLTVDERNERAIREELRRVHGPDAYARLAMPKILAARETGRVLIDGLYSWS
                     EYKYLHEALASRMLVLAITSARRIRYARLAGRSERPLTAEEARRRDFAEVENLEKGGP
                     IAMADYTIVNDAALDDLRREVDRAIAFLFPS"
     gene            48160..48495
                     /locus_tag="PKM94_03270"
     CDS             48160..48495
                     /locus_tag="PKM94_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65688.1"
                     /translation="MDERAIKKLLAQERQRWFGWGHEDWREMIEATPDDEDGELFFYL
                     APFGGEAFPVEVVCFPDPDGAMEISLIVTIEENGREAEAAWMVYADGEKEMLVETTDR
                     REGDEEGDE"
BASE COUNT         8099 a        15041 c        17549 g         7963 t
ORIGIN      
        1 cggcaacgac ctcgagcggg cgaccaagct ggcgcggcgg atggtgtgca actgggggat
       61 gtcggagcgg gtcgggccgg tgacgttcgg ccggacggag gaccacattt tcctgggccg
      121 cgagatggcg cagaccaagg attactccga ggcgacggcc gagctgatcg accaggaggt
      181 gcggcgtttt atcgagacgg ccgaggaaat cgcccgcggc attctcaccg cccatatcga
      241 caagctccac gccctgtcac accggctgct ggagaaggaa gcagtcgaca gccgcgaggt
      301 ggacgaaatc atcgggcgcg cgccgagcgc ggccgcagag gtggagtcgc cgacgccgac
      361 gccggaacgg tgagaaacgg cgccgggatc gggagtctcc cgccccggcc cccggcggtc
      421 aggattcaat agcgtggacc acgaaaaaat catggcgggc gtgcagctga ttctcgaggg
      481 gatcggcgag gatccgaacc gggaggggct ggtgcggacg cccgagcgga tggcggagta
      541 cttcgaggag gtgctcgccg ggtacgccca ggatcccacc caggagctcc gcaagtacac
      601 gacctccaac aaggacgaga tgatcatcat caaggacgtc tcgttctact ctctctgcga
      661 gcaccacctc ctgccctttt tcggcctcgt gcacatcgcc tacatcccgc agaacgacaa
      721 ggtggcgggc ttctccaaca tggtccgggt ggtcgagatc ctcgcccagc gcctccaggt
      781 gcaggagcgg atgacgaccg acatcgccga tgccatgcag gcggccatcg agcccaaagg
      841 cgtgctcgtg gtcgccgagg cggagcacct gtgcctgacc atgcggggga tcaagaaacc
      901 gggcagccgc atcgtgacct cggcggtccg ggggatgatg cggcggccgg ccacgcgcaa
      961 cgaagcgctc acgctcatgg ggcgcggaga gtaggggacc gggtccccgc ccgcgcaccg
     1021 atcagttgtc cgcccgccgc cgggcctgca cctccgaatc ccccatccgg agaaccgcgt
     1081 gcgacacgga gccgtcctcc gagagcacaa acgagagcgt gatctccgcc agcgggtagc
     1141 ggacgaagaa ggcgccggcc gcctcggcat acagcttcag ccggtcgcca taggggttga
     1201 tggcgaccag accatcggcg ccgaggatga tctcgatggc gcttccgggg gcgatttcgt
     1261 agacgccgac atacgattga agggtgctct cggggaggga cagcggctgt ttttcggcga
     1321 ctatgagccg ggccgtgcgg agggtgtcgg ccgccgcgta gcgggcgccc agcgccctga
     1381 tgcaatcctc tgagtccgag gcataggcca ccagttgggc cgcgctcaga gcgaggttgg
     1441 cgaccggctc tccggcaagc agcgcgtcct gcagtggcgc gcagatctcc aaactcgcgg
     1501 ggataatgaa cccgtcgcgc aacaccggca cctcagcggc ggtcagagcc gtgcggtgga
     1561 tcgtcaggcc gcgaaaatcc gaggcgcccg gacgagcccc cgtcccgggt ccgccgcccc
     1621 cgaggacaaa ctggaggggt tcgaggcagg ctgcggccgt accggccgaa aggccgtcca
     1681 caaacagctc cgtctcgcgt ttcaggtagc gctgcacgag cgcgatgtgg cgctaccggc
     1741 cgggcgccgc accggcggaa acgatcgccg caccgtcgcc gccgctccag atgacccggc
     1801 cgtcgccgtc aatcgccagg ctcgcggcgt gcgtacggga ggtgatcgcg gcgatagcgc
     1861 ccggtccgtc tgtccggact tggaaggcga acgtaaatga gtggaccggg tgtgacggca
     1921 cccagctgag cggaaggggg ctgtcggcgg tcccgaccgt gacgtacctt tccccggaaa
     1981 actccgtcac cgcttccttt ccctcccgaa gcgcatcgaa gatggtcggc acgatgctca
     2041 gggacaactc ctcgtgtccg aggtcgtccg ggtggccctc atcataggtg tagccgtcga
     2101 ggagccgccc ccggccgtcg tcaagggcgc cgaggaggtt gaggccgggg aggttccagg
     2161 cgtagagccg gaggttggtg cgctggaggt aggcatactg ggcttcggag aagccgctgt
     2221 tggggtagca cgaggcgagg attggctgaa tgccgcggtc gcggcagcgg gcaaccaggc
     2281 tgagcaggcc ggtttcgaag gctgcgcaga cggaatcggg gtcgagcgtc tcgaggccct
     2341 cgttggagag ggagagggcg atgatgagga aatcgggatc agccgggagg acgtcgccgt
     2401 cgaaccgggc gagagccttc gcggtgttgt cgcccggcac ggacaggttg accagccggt
     2461 agcggcgggg atcgagggcg cggccgaggc gggcggccca gccgttctcc atgtcgtacc
     2521 tggtctggaa cgaggtcacc cagccggcgg ccacggaaga tccgaggacg gcgaccgtga
     2581 gaacagaatc ggacgggcgg ctatcggccg ggtcgggggc ggcgccggcg gaaacgccaa
     2641 gggccgccag aaggaccaga gcgcagagcg gtttcatgag cagtctccct gcaaggtcgg
     2701 ttttccgagg gcgccacagc accccgcggg gctgcggctc atgcctcttt tgaccggacc
     2761 ggccccggtc aggttgcagg gaaaggaccc cggcgggccg ggacgagact gccggaggcg
     2821 ctcacgaacg ggccgaggcg cgctccataa acatcttcag cgccaccgca tgattgtggt
     2881 ctttgtcccg cgcggcatag accagggtga cggtccggcg ggtgtggagg atctcccgga
     2941 gctcccccac ggccggattg gcggccagct cggtgaaata gcggcggcgg aaatccgccc
     3001 atttttcggg atcatggccg aaccaggtgc ggagtgccgg cgagggagcg atctccttca
     3061 gccatcggtc gatccgggcg tcctgtctga ccaggccgcg cggccagagg cggtccacca
     3121 gcacgcgata cccgtcgccc ggcgacggcg gttcgtaggt gcgtttcagt ttcatccgca
     3181 gttacctcct ggccgagtaa ccgggcggcg gccgcggcgg ttccggcgac gccggcggct
     3241 ttgcattttg agtcttgcat ggcgcggcgg cgcggcgtta tatatcactc acggggacaa
     3301 aaaagcggta tgtccagaga aacgctgaca catgacggaa cgagcgggcg ggccattcgg
     3361 ctggccgacg gacggcgctt gtcgctggcg cggcccctga tcatgggcac gctcaacgtg
     3421 acgcccgatt ccttttccga cggggggcgg cacacctcgc ggtcgatggc gatcgggcgg
     3481 gcgctcgaaa tggaggggga gggggcggac attatcgacg tcggcggtga gtcgacccgg
     3541 ccgggggcgg cgccggtcga cgtagacgag gaaatgcgcc gggtcctgcc ggtgatcgag
     3601 gggatccgca aggcctcgga catcccgatt tccatcgaca cctacaaagc catcacggct
     3661 aaagcggcgc tcgatgccgg ggccgacatc atcaacgacg tctccgggct gcgcttcgac
     3721 gaggagatgg cgggtctggt ggccgcgcgc ggcgtgccgg tcgtcatcat gcacatgaag
     3781 gggacgcccc gggacatgca gaaaaacccc cgctacgatg attgcctggc cgagatcaca
     3841 gcctttttcc gggagcggat cgtgcatgcc gagcggcagg ggatcgcgcc ggcccagatc
     3901 atcatcgacc cggggatcgg gttcggcaag cggctggagg acaacctgga gatcctggca
     3961 aatctcgggc ggttccgcga tctgggtttt ccgcttctgg tcggcccctc gcggaagcgg
     4021 ttcatcgcga tgatccaccc gagcagcaaa ccggctgacc agcggctggg gggctcgatt
     4081 gcggcggtgg ttgcggcggc gctcggcggc gccgacattg tccgggtgca cgacatcgac
     4141 cagaccatgg aggcgctgcg ggtggcccag gcgcttcggg gagcggtccg gtgagcctct
     4201 tcagctgggg attcgtcagt ttcggcctga aagacctgat cgacgtcctg attgtctcgt
     4261 tcatcatcta cgaggcgctc aagctaatga aggggacgcg gtcggcgcag atcatcgtgg
     4321 ggctgttcct ggttgccgga gtggcgttca ttgcctactg gtttcaactt gagggtttga
     4381 cctggctgtt ttcgaatctg gccacgttcg ggctgatcgt gctggtggtc gtgttccagc
     4441 cggagctgcg cggagtgctc gcgcagctcg gccagcggcg cttcttccgg cggttcgtgc
     4501 agctcgagcg aaagaagtcg ctcgaggaag tgtcgcgggc ggccctgcgg ctgtcggagc
     4561 tgcgctacgg cgggctggtg gtgatcgaac gccgcaccgg gctgcggaat ttcgccgagt
     4621 cgggcaagct ggtcaacgcg gaactctcca gcgagctgct ggtcaccctg tttacgccct
     4681 acacgccgct gcacgacggg gcggtgatta tttccggcga gtatatcgtg gccgcctcgg
     4741 cgtcgctgcc gctgaccacc aacccgcgct accgcaagct gtacgggatg cgccacaagg
     4801 cggcgatcgg cgtgtccgag atatccgacg cggtggtggt ggtcgtgtcc gaggaaacca
     4861 ccggcatctc gatcgcctat aacgggggac tggagaaaga catcaagaag tccgagttcc
     4921 gcgacaagct ggcatcctac ctggccaggt gatcggatcg tcgggctttc cgcgcaagtt
     4981 cgcacttgac agccggtcgc cggggggcgt ttctttttcg gcagacgccg ggccggcgtc
     5041 gcgggcgacc ggagaccgcc cggaccggga gacggctcgg ggtcgcacgt tgcaggacac
     5101 aacctgtaat tgcgggagag aggtatggca gggaagtcgg agtcgatcgg gtcgtttctg
     5161 ctgaaagctt tgatcctgct ggtggtcatc gccgccggcg tgtttgccgg attctggatc
     5221 tcatcaaaaa ccgggctgac cggagtattc ggcgggaacg ccgcacccca ggaaatgatc
     5281 aaccgcagca acgtccgggt cggcgacatc ttcccgccgg tcacggtgcg gcgggacggc
     5341 gccgagacgg atatccggac gctcctgctg ggcaagcggt cgctggtcgc gttcgtgtcg
     5401 ggcgcctgcg agccctgccg gctgctggtc gaggaggccg cgcggtggcc gagcgtgatc
     5461 gagggaaacc tgcaggtgct cctgctcacg cggacgccgg aggcgcaccg ggggagcccg
     5521 ctcttgcagg tgtacgagat cccggccgag gttctcccgc agcttgagat cggtgtcttt
     5581 cccacgctct acgtgatcaa cgaggcgggc gaggtgcggg tggcgctgtc ggggtacagc
     5641 gaagcgatga ccgaggaggt cctcaccggg cggctgtagg ggagcggccg gcgggcgcct
     5701 ctttcttgcg tttgacattg ggcgggcggc cccgtatctt ccgccgctgt gactgcagcc
     5761 gagacggaga gatgacgacg ccgctgaaga atatacgcaa tttttccatc atcgcccaca
     5821 tcgaccacgg caagtcgact ctggccgacc ggcttctcca ggtcaccaag accgtgtcgg
     5881 agcgccagat gcgcgagcag gtgctcgact cgatggatct cgagcgggaa cgggggatca
     5941 cgatcaaggc gcacgcgatc cggctggagt atcgggccgc ggacggcgcg gtttaccagc
     6001 tgaatctgat cgacaccccg ggccacgtcg atttcaccta cgaggtgagc cgctcgctgg
     6061 ccgcctgcga gggcgcggtg ctggtggtcg acgcctccca gggaatcgag gcccagacgc
     6121 tgtcgaacct ctacctggcc atggagcacg acctcgagat actcccggtc gtgaacaaga
     6181 tcgacctgcc gaacgcggag acggagaagg tggtcgcgga gctggtcgac ctgctcggcg
     6241 tgccgcggga ggaagtgctc cgggtttcgg ccaagcacgg gacggggatt accgaggtgc
     6301 tcgaggcgat cgtgcgccgg atcccggggc cgaaggggga tccgggtgcg ccgctgcagg
     6361 cgatggtgtt cgattcgatt tacgacagct accggggggc gatgccgctc ctgcggctgt
     6421 tcaacgggac gctgcggacg cacgaccgca tccgcgccat ggccacgggc aagacgtacg
     6481 acgtcgacga ggtggggtac ctgcggctcg gcacggcgcc ggcggaggcg ctcgtggccg
     6541 gcgaggtggg gtacctgttc gcctcgatcc gcgaggtggc cgacaccaag atcggggaca
     6601 ccctcaccac ggtggagaat ccggcggccg cgcccctgcc gggctttgtc aacatcaagc
     6661 caatggtgtt ttcgggcctc tacccggcgg tggccgagga cttcacgcgg ctgcgggagg
     6721 cgctcgaaaa actcgcgctc aacgactcct cgctctcgta cacgccggaa tcctcgcagg
     6781 cgctcgggtt cggcttccgg gtgggatttc tcggactcct gcacatggag attgtcaccg
     6841 agcggctctc gcgcgagtac gggcagttga tcatcaacac cgtgccgaac gtcgaatacc
     6901 acgtctaccg gaccgacggg acgatggcgg cggtcgacaa cccggcggac atgcccccgc
     6961 cgcaggagac cgaccacgtg caggaaccgt acgtcgacgc ccagatcatc gtcccgagcg
     7021 aatatgtcgg ggcgatcatg aagctggcga ccgagcggcg cggcgagtac aagaacaccg
     7081 agtatctctc ggccgcgcgg gccaacctgt cgtacgcttt ccccctgtcg gagatcatct
     7141 tcgatttcta cgacaagctc aagtcggtca gccggggcta cgcctcgttc gactacggcg
     7201 tgccgtacta ccggcgctcc gatctcgtca agctcgacat cctcgtcaac ggagagccgg
     7261 tcgatgccct gtcggtcatt atccaccgcg accgggccta cagctggggg aagagcctca
     7321 atgagaagct gcgggagttg attccgcgcc agatgtacga ggtcgtgatt caggcggcga
     7381 tcggcagccg gatcatcagc cgggcgaccg tcaaacccat gcggaaaaac gtgaccgcga
     7441 agtgctacgg cggggacatc acccgcaagc ggaaactgct cgagcgccag aaggagggga
     7501 agaagcggat gaagcagttc gggcgggtcg agattccgca ggaggccttt ctggcggccc
     7561 tccagatcga gcggtgaggg gaacggcctg tccggccgcg ccgatcaacc cgcacgcgca
     7621 ggattcacca ccttgcccat gaacaggacc gcgcccgatt ccgcctcatg gatgataaag
     7681 aggaacgggc ggtcgacccg gaaaagcggc ggtaacgacg tcacccccca gccgatcgag
     7741 gtcacggccg ccgcctccgt ccctttctca tcgacctcca caaacgtctt gtgcttcacc
     7801 tcgccgatat acgacccgac gtcggcgaag aggcggcgga agtcggccgc ttccggatcg
     7861 aaagcagtgc gcatgcccat gcgcgtgagg atgtcgttga gtgtcgcctc gcaggcgaat
     7921 ttgtagcgcg gcaggagaat ctcgaagtcg gcgacggtcg gcaggctcga ccacgtccgc
     7981 caggtgtccg ggtccagcag gaccaccagc gaatcgaccg ccacccatgg gtgcggcagg
     8041 aggatggtca tccggaaacc ggagcggccg tagggcaggg tcgccgcctg aaacaactcg
     8101 tgctcgccgt acatcatcgg gcgcgcggcg aatctcccgg gatcctgctc gatctcatct
     8161 gccgtccgct tccacatcgt ggggcagagc acagtcgcac cgccgccggg gtggaagggg
     8221 agttcccggg tgaagtcctc gttgaaggga taggtccagc tcgccttgaa acagagggcg
     8281 ttgatcagga acgcccccat ctccggactt atcggcgatt cgatgatctc ggtgatgcgg
     8341 ccgccggtgg ccccggccac ccagctattg atggtgtcgg ccgcccacgg ggcgttgaaa
     8401 tcaagctcct ccacccgggc atcgaaatac tcgcggccca tgtcggtgaa actcggctgc
     8461 acgcgcagcc ccagccgact ccagaaggag ttggcgacgg tgaaggtaac ggccggatcg
     8521 gccccgctga ggatctcggt caggccgcgg taggcctcgt tggtttcatc gggcgaggac
     8581 ccggcgagtt cgagggtggc ggccatttca tcgcgggtga ggccctcagc gccgttgaga
     8641 gccaggccga gcgcgtacga caccgacagg ggggagacga agatgttctc ccccgccgca
     8701 gcgttttcgt tcacgaggcg gaacaacttg aatccgaaac ggttgacgga ctgcacgagg
     8761 tcggtttcgg ggcccgtcaa ctgcgccggc gtgcgaaccg gcgtcggatc atccgggctg
     8821 gtcggctcca ccgggtcgga gcactgcacc gccaggagga cggtgccgat gagcagaaaa
     8881 gcaaaggacg tttttatcat ggcaatctcc cgggttttgt cggccgccgg cgttcgtctc
     8941 tatgctatag atgcagatac ggggctgttg gtgcaacgga aaactgggcc tgcggcggct
     9001 gcgggaaacc ggcgtccccg gcgggagtac aatcagtaat tgccaacgga ctaatttgtc
     9061 gtaagttagg gactatgaag agactcctct gcaccctgct tttcatcggg ttgcccttgc
     9121 cggcggcagc cgcccggctc ctgattccca tggacgactc gcagaccgac cacctcaaag
     9181 cctacggagt cgtgtttcag gggctggaaa aggggtcgaa aggggaatgg ctgctcaact
     9241 accgcggggg atcgttcctt ctggactaca accaggacaa cgtcaaccgc tgtctcctca
     9301 tgggcgtggc ggccgagcag gtgagcgagg gacaggtcgc cgacatttac cgccagatcg
     9361 aagcggggaa tatggagcgg gtcgaactgg agaaggcgcc gtcgatcgcc gtctactccc
     9421 cgccctcggc gcagccgtgg gatgatgcgg tgacgctcgc gctcacctat gccgagatca
     9481 agtacgccat cgtgtgggat gaagaggtgc tggcgggcgc gctcgacgat tacgactggc
     9541 tgcactgcca ccacgaggat ttcaccgggc agtacggcaa gttctacgcg gccttccgca
     9601 acgagttgtg gtaccaggcg gacgtgaaga ccaacgagga catggcccgg cggctgggct
     9661 acatgaaagt gtcggacatg aagctcgagg tggcgaaagc gatctacgat tacgtgcggc
     9721 ggggcgggtt cttgttctcc atgtgctcgg cgccggagac gatcgacatc gcgctggcgg
     9781 ccgagggcgt ggatatctcg cccagcgagt tcgacggcga cccggtcgac ccgaactgcc
     9841 aggcccggct cgactacagc aagacattcg cgttcgagaa tttcaccgtg gaactgaacc
     9901 ccctggagta ccgccactcc gacatcgaca cctatccgga ccgccagatt cgattcctca
     9961 gtccggacga ggactacttc ttcctgttcg attttgcggc caagctcgac ccggtgccga
    10021 caatgctggt gcaggatcac acggcgacgg tggccgggtt catggggcag acgaccggtt
    10081 tccgcaagtc gctgctgaag gatttcgtga ccattttggg tcagccggac aattacgatg
    10141 aggtccggta catccacggc aatctcgggc gggggacatt cacgtttttg tccgggcacg
    10201 atccggagga ttaccggcac atggtgcacg atccggcgac cgatctcgcg ctctacaaga
    10261 actcgccggg gtaccggctg attctcaaca acgtgctgtt ccccgcggcc aagcggaagg
    10321 aacggaagac ctgagggtat ggcggcggga ggcgacacgc gcactcttct cgggattgat
    10381 tacggcgccc gccggatcgg ggtcgccaag agcgatccga ccgggctgat cgccacggcg
    10441 ttgacgacgc tggtggtgaa atcggcggcc gacgcgctgg cccaggtggc cgcgctgatc
    10501 cgcgaacacc agcccgcggg catcgtcatc ggatccccgg tcgggctgtc gggcgagtcg
    10561 aaccggaagt gcctcgagat cgaggccttc gcgaacgcgc tccgcacggt tttcgacggg
    10621 ccgatccacc tgtgggacga gggctactcc tcggtcgagg cggcggacat catccatgcc
    10681 cacggcaagc gggtcggcaa ggacaagaag cggatcgatc ggctggcggc ggtgatcatt
    10741 ctgcagcggt ttctcgatga gcagccggaa cggtaggggg gtgtgacaga gggagcagct
    10801 ccggcgggtg gcggcggtcg cccggccggt cctcgcggca gaaattatgg gtagaagaat
    10861 cgtcacggcg gcggccgtgc ttttggcggc ggccatcatc gggctggcgg tcggacagca
    10921 ctatcgcacg gtggacctgg gagaccgggt ggcgaccgtg atcatcgccc cgggcgactc
    10981 gttcgcccag gtggccgccc ggttggacgc ccagggagtg gtgccgtcgc cgcgcctcct
    11041 gcggctcgcg gcgagactca ccggtgtgga ccgcaggctg atccccggcc ggtatgattt
    11101 cacggggaaa cagtcggcgg ccggcgtgct cgaccgcctg gcccgggggg atttgctccg
    11161 catccgggta acgattcccg aaggcacgac gctctggcgg acggccggtc tgctcgcccg
    11221 cagcctggaa cttgattcgg cggcgatcgt gggcctgggc agcgacagcg cgtttctcgc
    11281 cgaactcggc aaaccctgcc tggagggata cctcttcccc gagacgtact tcttcccctg
    11341 gggaatcgac gagcgggagg cggtgcggac ggccgtggcg cagttcgacc ggcagaccga
    11401 gggactctgg gcgggggaga ggacgctggg gctctcgccg cacgagattg tcattctggc
    11461 ctcgatcatc gaatcggaaa cgccgctggc cggcgagcgc gggctggtgg cctcggtcta
    11521 tgctaaccgg ctgcggcaga agatgcggct ggatgccgac ccgacagtga tctacggtct
    11581 ggggggactc gatcggccgc tctcccggcg cgacctgcgc aaggacacgc cctacaacac
    11641 ctaccttcac gcggggcttc cgccgacgcc gatcaactcg ccgggactgg cctcgatcaa
    11701 ggctgcgctc gctcccgaca cgagcgacta cctgttcttc gtggccgaca acgcgggggg
    11761 gcacttcttc tcgaaaacca atgcggagca tgagcgggcg cgccggcgga tacggcagga
    11821 gcgcggccgg taggcgctcg gccggaggcg gttcaggcga ggcgctgctt tcgtatcgcg
    11881 gccgcgaagg cccgggccac atcctcgacc gccccttcct cgaacatcgc aaaggcggtc
    11941 ggttcgtccg agctgtaggc caccaggccg accacctcgc cgtcgctggt gagcggctgg
    12001 acgacaaagg agcgggactc atcgccaagc aggagcttgc gctcgaagaa gttgccgctg
    12061 aaggaggcgc agaagttctc cgtgtagacg cggccgtgtt cggcgacctt ttcaaacagc
    12121 gaggactgcg ggggaaggtt gaccgccaga ccgtcgcgga gcgatccatt cacccaggtg
    12181 gagacggcgg cgaaggggcc ggcggcgccg cggcgcatga tcagcacgcc ccggttgatg
    12241 gcgtagcgac gggcgagctc gcgcgtcagg tgggcgaaca tctggggcgc ccccgccccc
    12301 tgttcgtgca ggacggcgaa atcgaccgga atcggccggg tcggggcggg cgtccgcagg
    12361 tatgaccgct tgcgcggcag cggcttatcc gtcatcgcgc acagcctcgc cggcgacgcc
    12421 cggtttcaag ttgtacttct tcactttccg ccacagcgtg gtgcggccga taccgagttc
    12481 ctgggcggtc tgggtgtaat tccagttgtt gtcgatgagc actttctcga ttaggtcgcg
    12541 ctggttgtcg gccaggcgca ggctgttggt ccgaggggcg ggcggccgct cctcggcagc
    12601 gcccggacgg tgctcggcaa acacgacggc gtcggaatcg atcgtctcac caccggcgag
    12661 ggcggcggcg cggcggaggc agctctccag ctcgcgcgcg ttgcccggcc agggccagtg
    12721 gagcaggcgg tcgagggcgg cccggctgat gtccaggcgc gttcgcccgg tttccgcggc
    12781 gatgcgccgg agcatagcgg ccaccagcgg ctcaagatcc tcaatgcgct cgcgcagcgg
    12841 cgggacagcc accgtgatag tgtccagttt cgccagcagg tcgcggcgca caccggcggc
    12901 cgccagttct ccggccgaaa gagcggaggt ggagagccac cgcacattca ggcgggcggg
    12961 acggccgggg aggacgccgg ttttgaggac ctcggcgagc cgttcctgaa ccgccgccgg
    13021 catccggtcg aggcgcctca ggagcactgt cccgccgtcc gcctgaacca gcagcccggg
    13081 gcggtcggat ccctcggagc cgaacaactc cgggaccagc accggttccg gggcggtgca
    13141 gtcgacgacg acatagggcc gctggcggcg ccccgagtgg tggtgcaaga tgctcgcgca
    13201 gagctccttg ccggaaccct ccggaccggt gatgagaacg gcgatgtcgg tcggggcgag
    13261 gcgggcgagc gcctcgcgaa gctgctgggc ggcccgcgac acgcccacga ggttgtcgaa
    13321 actgtagtgc atcgcgacgt gctggcgaac ggccgcgagg cggactttga gttcccggtg
    13381 ttcgatggct ttccgggcgc gcgccaggag cgcctccgtc gacggcggtt cggcgacgca
    13441 gtcgaaggcc cccgcctcga gggcggccgc gatccggccg gggggcgcgg cgggaagcac
    13501 caggaggatc tccaccgccg gatcgaggcg gcggacccgg cggatcagct cctgcggatc
    13561 gggcagggcg gcgtcgtgac ggaccagcac caggtggatc tgcccggtgg cgagcacggc
    13621 cagcgcggaa tccagcgtgt tgacctcggt tatttccagc gtgttgtcgg ccaggggcag
    13681 ggacagcgac cggcgctcgg tctgaccggg atcgagcacc agaatgcggg cggcggcggg
    13741 acgaacggtg atcatgcggg tatctcctgg gtgcggaagg ttacagcggt agtgagggag
    13801 cgggaggggg cgtcgacacg gcgctcgacg gcggcgccga ggcgccggac caggcggtcg
    13861 gcgtcggccg gccaggcccg ttccaggtcg gcgatgcggg cggcatcgga aacgatcgtg
    13921 gtgaagacca cccgggcctc cggcgcgccg tctgcgcagt ccacccgcac ggcggtggcg
    13981 gcggcgcggt cggccgcggc gtgcgcctcg aggacggtct caaataggac cagaagggcc
    14041 tcgggcggag ccgagaccaa aaactgctcg cccccgccgt cgagcaccag cgagatccgc
    14101 tcccggtcga ggccggcgaa gcggtcgatg gcgtggtcga tctggtgcgc gaggttgacg
    14161 cgggccggct ccatctggcc gtagcgggcg accagcgagg tgcgggccag ccggcgcgag
    14221 agcgaccggc cggtgcgggc gatctcggac gccagcgcct ggatttcccc ggccgcgagc
    14281 agaccgctgt tgccggcgat gtactcggcg gccgtggtga gtccggcggc gtcgcgccgg
    14341 agcgagtcca cgcaggcgag cgacagtctg gtctcggcgg cggcctcgcg ggccggctcg
    14401 aaagtgaggc aggccacgtg gagacagccg caggcgtgat tttcgagttt catcttgaat
    14461 cgggcgacca gccggcggac gagcggctcg accgtcccgt actcgcgcgc gacgagcgcg
    14521 gacggttcgc aggcggccgc ggtgcagatg tccgggtcgg cgtggaagac ccgccccgag
    14581 gcgcgattga cgaggatgcg ggccgccccc ggcggggtcg gaccgagcgc ggcgagcatc
    14641 ccgcgcacgg aatcgcgcgc gctgcgaaag gcgctgatga cccgctcgaa gcagcgttcg
    14701 agcaccacct tgtcggtcag gctgtcgggg cagagcatgc ccaggaccag cggcgctccg
    14761 ttgcggtcct cgatcacacc cgtgaatacc cggctgatgc ccggccccga cgcggcgccc
    14821 gccgcgcagc gatgcgagaa gacggcgtcg cggccctggc gaacggcggc gtcaatcagg
    14881 tccggcggga acaggggaac ttcctccgga tgcaggtagc ggtcgccgcg cacgaggaag
    14941 gtgagggcgt tgtcgggagc gaggctgtcg aggtactcct gttcgctccg gctcagccag
    15001 ccgcggatct caacgccgag caggagcgac gccgcgcgga cgacggcgga ctcctcggag
    15061 accgccgtcg ggaaccgctt cgattcgaaa cggtcgaggc gctcgagacg ggactgtttc
    15121 gaagtggtcg ctgtcgtggt catctttggg acatccttgt ttcaacttga atcatctgct
    15181 agaggtatcg gccccgggga ggaggaactt tagggcaggg tggagcccgg gggacgggcg
    15241 cgggcggcaa aaaccttgac aggccgcggg cgccgccgta tcatcgctca tgccgctcga
    15301 cagcatccag atcggccgcg gagcacggcc cccccgaccg aaaactccca ctcccctgac
    15361 catagaatcc ccgtttcagc ggctcggagc gcggtggtcg cgccatgcct tgggggcagg
    15421 tcgtcatgcc gctaatccct tgttgcagaa tatgataaac gtcggtagcc cgggtctcac
    15481 gatggacgtc ggccagggag attggcggcc ggaggaaccg ggtgatccgg ccggggagga
    15541 tcaaatggcg ggggtagtcg cgatcattcc tgcgcgcctg gggtcgaccc ggtttcctcg
    15601 gaaggtactc cacctttacc gcgggcggcc gctgttgtac tatgtgtggc gcgaggtcag
    15661 ccgggcccgg cggatcgacc gggtggcggt ggcgaccgac tcccgggaga tcgtagcggc
    15721 ggtcagggaa ttcggaggcg aagcggtgcg tacgtcggcc cggcaccgga ccggatcaga
    15781 ccgggcggcg gaagcggccg agaagatcgg cgggcggatc attgtgaatg tgcaggcgga
    15841 caacttcggc ctcaccggcg cggcgctcga ccgcgtggtc gcgcggatgg agcaggatcg
    15901 gacgatcgag ttcgcgacac tggcatttcc gatcggcggg gcggaggatc ttgcggaccg
    15961 gaatcttgtt aaagtagtgg cgtcggaccg ggaggggagg gcgctgtggt tttcgcggtg
    16021 gccgattccg ctggtgcgcg acgccggggc gcgctaccgc cacctcgggc atatcggagt
    16081 gtacttcttc cggcgggccg ccctccggcg ctatgcggag tggccccgga cggcggccga
    16141 acgggccgag tcgctcgagc agttgcggat tctggagcac ggcggccgga tgctggtgtt
    16201 tgagacgcgc gcgcggacgg tgtcggtgga cgctccgccg gatgtggcaa agttggatac
    16261 gctatacagg tagaggatgc gatggcggac aagaccaaat acattttcgt gaccggcggc
    16321 gtggtctcgg ccctgggcaa ggggatctcg tcggcctcga tcgggctgtt gctgcagcgg
    16381 cggggtctga cggtgtacaa catgaagctg gacccgtacc tcaatgtgga cccggggacg
    16441 atgaacccgt accagcacgg ggaggtgttc gtgctcgacg acggttcgga gacggatctc
    16501 gacctggggc actacgagcg gtttctcgac cgctcgctca cccgcgacca caacgtcacc
    16561 accgggcaga tctaccacac gatcatcacg cgcgagcggc ggggggatta cctcggggcg
    16621 accgtgcagg tcatcccgca cgtgacggag gaaatcaaga accgcatccg gcgcctggaa
    16681 cggcttccgg agaagccgga cgtggtgatc tgtgagatcg gcgggacggt gggggacatc
    16741 gagtcgctcc ccttcatgga ggcgatccgc cagatggggc tggagcaggg gtacgacaac
    16801 gtcatgtacg tgcacgtcac gctcgtgccg tacatcgcga cggccggcga gttcaagacc
    16861 aagccgacgc agcattcggt gaaagagctg cgggccatcg ggattcagcc caacatgctc
    16921 ctgtgccggt cggccaagcc gctcgacgaa gggctgcgcc agaagatcag cctcttctgc
    16981 tccgtgccga ccaaatcggt catcgccgcc gaggacgtct cgacgattta cgagatgccg
    17041 ctgcggttcc gggcgcaggg aatggacgat atcatctgcc gccatttcgg gctggagacg
    17101 ccggagccgg acctgggcga ctgggagcag atggtggagc ggatcagcta cccgacccat
    17161 cggctcaaaa tcgcgatctg cgggaaatac gtgatgctca aggacgccta caaatcgatc
    17221 atcgaggcgt tcgtccacgc gggtgtccac aacggggccg aggtcaagct catctgggtg
    17281 agttccgaag agatcaaaca caacggcgcc ggcaagttcc tccacgacgt cgacggcctg
    17341 ctgatccccg gcgggttcgg ggagcgcggg gtcgagggga agatcgaggc tatccgctac
    17401 gtgcgcgaga acagaatccc gtttttgggc atctgcctcg gcatgcagtg cgccgtgatc
    17461 gagttcgccc ggaacgtctg cggcctcgag gacgcccaca gctacgaatt ctaccgcgac
    17521 ctgaaacacc cggtgattca cctcatggcc gaccaggaag gggtgaccga gctgggcggg
    17581 acgatgcggc tgggggcgta cccgtgccga ctggccgcgg gctcgctcgc ccgccaggcc
    17641 tacggcgcag acgaaatctc cgaacgccac cgtcaccgct acgaactgaa caacgcctac
    17701 cgggagatgc tggggtcgca cggcctgcgg ctgagcggga tgtcgcccga cgggcggctg
    17761 gtggagatca tcgagcatcc cgagcatccc tggtttgtcg cctcgcagtt ccacccggag
    17821 ctgaaatcgc ggccgacccg gccgcacccg ctgttccgcg actttgtcaa ggccgcggtg
    17881 gactacaagg tgcggcgcaa cggcgcctcc ggatcgaaca acgaggaaga gcacgacctg
    17941 tccgaggcgg cgcgctgacg acgcgcgggt gggaggagga ccgaggatgt cgagagcggg
    18001 gaaggcgcgt ttccggccat tgatcctgct gctggcggcg gcggccctgg cgggggggtg
    18061 caaagaggac cggcaggcgc tcgcgcctcc ggaagtgtac acggggatga tggtgatgga
    18121 gccggatttc gcctcgggtc ccatggagat ctacccggac tccaccatgg tgacgttcac
    18181 ggtcgagggc acactctacg agctgctgct ggaggacgag acggattttc acctctgcca
    18241 cacgcggggg acggtgcggt cgttcgggac gaacgcgctc acgctggctc ccacggggac
    18301 tttcggcgcg ggcaactgcg attcgatccg cgtcccgcga ggggaactcc cgaccgtgtt
    18361 ccggggggac agcctcttca tcgggccgaa agacatcgcc tacgagattc acatcaacaa
    18421 caccaccgtc accgacatca tgcgctacac gttccggctg aggcgggtgc agtaggggcg
    18481 gcgcggggtt cggcccggcg gtcacatcag gacgccgcgt ttgtaggcgt cgaggaagac
    18541 ccccagcatg agcgccaggg cggcggcggc gtagaggacc gccagcgggc ggcggcgcga
    18601 gcgcagcgac aacccgacca tcgcaaacag cggaaacgcg accacgacgt ggcgatagta
    18661 gcaggcagtc gtccccgcgg ccggagtgaa cagcagcacg ccccacagga aaacctgcca
    18721 ggcggccggg atccggcgcg tggagaagag cgcgagcgcg gcggcgacga aaatcagcat
    18781 gacacggacc gggtgggcgc cgggcaggtg cacgaccgca tcgaggatcg ggagcagcgg
    18841 tacggtcgcc ccgtgggcgt agtacggctt ggactgaaag tgcacataga ggaagaagtc
    18901 gccgaaagcc agccggtagt agagcgcgta gaggatcagg gcgagcacga ccgcggcgga
    18961 ggcggttcgc aggccggccc ggtcgcgggg cgtaagccct cgccagcgtg agacgagaac
    19021 ccacagcagg gggaggatga tcgcgacccc ggtggggtag gtgatcgcca gcagtccggc
    19081 cggcgccgcc gccgcgaagt agcgctcctt ctccacaagg aggaagacgg cggcggccag
    19141 cagcaggtac agggcatagg ggaaggcggt gaggtagtag aacgaggtgg ggaagagcgc
    19201 gagggcggcc agcgacccgg cggcgacggt gtggtcgaaa cgccgggcga acaggcggta
    19261 cagcaggagc atcgccgccc agaggcagag ccacccggtg atcagcatcg accaggccat
    19321 gccgaggccg agcgggccga gcgcaacact caccacccgc gccgccagcg gaaacagcgg
    19381 gaaccacccg acattgccgc acacgagcga gggatcctcc gggcagggaa atttctcgta
    19441 cccggtctcc gctatcgaca gatagtgccg gccgtcccac tggatcagcg tgcgcgccag
    19501 cggcgcgtac ttgcgctccc ccgtcccgag aagcgcgagg acgaccgcga ggaagaggaa
    19561 agtcacggcg gtcgggacga ggaaactttt actgcgcagg cgggcgaaat tcattaagtt
    19621 gttccgttcg cgcgacccgg cggggccggc gcggatctcc ggccgcggat cgcggctctt
    19681 cggactctcc cggcgcgacc gacgccggcg gcgccggccg ataccggaca ggatggaaag
    19741 tagcatcccg gatggacctg cgaaagccca aagttgcgac tgttggtctc tcggccgtcc
    19801 tggcgttgta ctacgcgctg ccattccttt tgaagctgac gtggtggggg gtgcgggact
    19861 gggacttgtt caccaccatt gccgctgttc cggtggggac cgtccttcac tacggccagt
    19921 tccccttctg gaatccctat ctcggcgggg ggaatatcct ctttcatcat cccgaggtcg
    19981 cggttctgtc gccctttgtg ctgctgtatt ttctgtgcgg gccggtggtc gggctgaaac
    20041 tccaggtgct gatcgcgtac ttcctcggat tttgggggag cctgcggctc tttcgcacgc
    20101 tggggatgcc ccggggcagc actgtcgtgg ccgccgtcgc gtatttcggg tcggtgcatt
    20161 tcgcgctcca ttttgccgag gggcacatgc cgttcacgca ctacgctttt ctgccgtggt
    20221 ttgtgcactt tgtcctcaag ggagcggaag atcgccgcaa cctcttgggg gcggtcgtcg
    20281 cattggcgct catgatcctc ggcaacggcg cggccgtgcc gcttctgtac acgctgctgg
    20341 cgaccgctct gctgctggcg ttgcgggcgg cgcagcggcg ctcgccggcc gaggcccggg
    20401 tgtttctcct ggcggtcggc ctcgcgctgg ccctcgcggc ggtgaaggtt ctcccgatga
    20461 tcgcgtacct tgcccagaac cgctggacgg gcgacccggc ggaggcgatc ccgtggtcgg
    20521 cgctgccggc gatgtttttt ggtttgaagc actcgctgtt cgtgcagaat ttcgccggcc
    20581 agaaatgggc ctggcatgaa tacggcgtct acgtgtcgcc gctgcttgtg gccgcgggag
    20641 cgtacgccgc cgtggcgcgg ttccggcggc attggccctg ggtggtgttc attgcctttt
    20701 tcctgctcct cggactgggt aacttcgctc cgtggtcgcc ctgggcgctg ctcaccggtt
    20761 tgcccgggtt cgagtcgctg cgcgccaccg gccgcgccat gcacttcgtc atcctcggag
    20821 cggctgtgct cggcggactg ggcctgggct gttgggagga ccgggcccgg gcggcggccc
    20881 ggggagggtg gctgcgggga gccgtggtcg cggccgggct gctcatcgcg gccaccaacc
    20941 tggcgctggc ctggccgatc atgtccgagg ggtttcggaa gccgccgaaa cacgtggatc
    21001 gatctccgga gttccgccag gtggtcgacg ccgagccgca cgcgttcgaa aactacctcg
    21061 ccaaccgggg ctcgctcgtg tcgccgtggc tctcggccta ccatcccagc cgggggctgg
    21121 tcggccccgg catagtgtat tcggagctat tctcgggcgg ggaggcgaaa gtctacgttc
    21181 gggactacac cccgaatcga atcacttaca ctctcaacgg cggcggccac ggcggggaac
    21241 tggtgatcgg gatgggctac gatccgggct ggcgcgcgac cgacggccgg acaatcacgg
    21301 agaatcaggg actgatcgca ttcccggtca cgccgggacg gcagacggtt gtcctggtct
    21361 accgaacgcc ctacttctct cacggggcgg tcatttcgct tttgacgctg ggcgggatgg
    21421 ttctgtggcg ccggcgcctc ttcggccgcc cggggcgcgg ggcgaatctg tagattcgga
    21481 tagccgttga cttcgccttg ggcgtgggtt atccttggcg gatgcagcga cggggttaac
    21541 cctgcgctca caccttgtgc ggagtgttcg gaatcatgcg tcttgtgtcc accatactgg
    21601 cggtgctgat ggtgacggcg gcggctgcga tcgcccagcc cgaccgggcc gattccgtcg
    21661 gtgccggcgg ctaccgcttt ttcgacatgc ccatcgaccc ggagcgctat ctgctgcggc
    21721 cggatgaacg gctgcaggtg acttttctcg ggacggcgct ggctccgctg acagtgcggg
    21781 tcaatccgga ggggagaatc gtgcatccca cgctcgggtc gttcgatctg aggggcgcga
    21841 cgctcgcgga ggcccgggtg gcgctcgtga aagcgctctc gtccctgtac caggccgact
    21901 cgatcgacat ctcagttctc gcgccccgcc gcgtggctat ccaggtcagc ggcgccgtgc
    21961 agtcgccggg gctctacacg gtctatacgt cgcagcgcgt ctcggaggtg atcgactcgg
    22021 ccggcggcct tagtccgagc ggctcgcggc ggctgatccg gctcctgggc ggtccccggg
    22081 agatccgcgt cgacctggac cgcgcgctcc tgggcgacga gcggtggaat ccgccgctct
    22141 acgccgggta cacggtgatc gtgccggcga aatccgcccg cgtcgtcaac gtcgtcggag
    22201 cggtggaccg tccccgcgat atcgagatcg tcgacggaga gtcggtcgcc gagattctcc
    22261 ccctcgtcgg gcccttccgc tcttcggccg acaccttggc ggtgcagatc atccgcggtt
    22321 cggaacgcct gccggcggcc ggtgtgccgc tccaggcgga ggacattgtt tttgtcccga
    22381 cgctctcccg gctggccgag ggcgcgccgg tgaccgtgta cggagcggtc gcgaggccgg
    22441 gccggttcct cctgtccgcc tcgggggcgc tgagcgagat cctggacgcg gcgggagggt
    22501 ttgcgcctga cgcggcgcgc tcggagctca cggtttttcg gcaagtcccg ctcgaggccg
    22561 cgctgcgtgc gggtgagcgg tattcgatcg ccaacctcat tgccctcgac ggcgcggtcc
    22621 agccgttcac gctgcagccg ggcgactcga tcttcgtccc gatgatggtc ggctatgtga
    22681 aagtgagcgg cgaggtgcgc aacccgggca tgttcccgtt ccgcgtcggc gggacggcgc
    22741 gtgactacgt gagggccgcc ggcgggtttc tcccgcgcgc cgaccgcgaa atggtcgaga
    22801 tcttccaccg ggtgtcgcgg ctgaccgaag agcatccggc ggaagttgtc gtccacgacg
    22861 gcgacgaggt tatcgtccgg ctgcgggagg aattccgatg aacgaccgcg ccgccgcgaa
    22921 caatttctgg cttttcctgg agctgctggc gcgccgccgg acgttcatcg tgaccttcgt
    22981 cctcttggcc acgctcgcgg ccgtgatcac cgctttcgcc ctgcccaagt ggtaccgggc
    23041 gacggcgctg ctgttgcccc cgaaagacgt cacgctgccg acgccgggtg cggggcgcct
    23101 cgaggacgtc gtgtcggtca cgaagggttt gaacctgccg gtgatggtga cgccgtcgga
    23161 tgtctacgcg cgcatgctga aaagccggac gatcgcccgg gagatcgtcg aacgcttcga
    23221 cctcatggcg ctctacaagg aagacaattt cgtcgagacc tacgacgtgc tgatggaccg
    23281 ggcgagattc cgggtgacgg aggagggact gctggagatt tcggtggagg accggaacgc
    23341 ggcgcgggcg gccgcgattg cgaacgcctt cgtcgacgtg ctcgatagca tcaaccggga
    23401 gattgtgacg gggcgggtgc ggcagaaccg gacgttcgtg caggaacggc tggcggtcgc
    23461 gaaggtcgag ctggattccg cccgcgccca gttccgggag ttccagatgc gcaaccgggc
    23521 gatcgatttc gatgagcaga cgcggttggc gatcgaccag gcggcccggc tcaagagcga
    23581 cctggcctcc ctcgacatcg acatcgccat gaaggagcag acgctggggg ccgacaacac
    23641 cgacctgagg gagctgaagc gccggcgggc cactgtccag gaccgtctcg atcagctcga
    23701 gcggagcaac cccgacagct cgttcttctc gctcccggtg tcggccattc cgacgctccg
    23761 cgggcagtac gaagtgctct acagccgggt gcgggtggcg gagtcgattt accggattct
    23821 cctggagcag ctcgaggaag tgaagtacca ggagcaggag aagcagccgg ttctgtcggt
    23881 gctggaccgg gcggtcccgc cggagatcaa gagccggccg cagcggtcca ttattgtgct
    23941 cgcggccttt gcgctggcgg tgctcgcggc ggttctcctg gcggcggcgg tggactatgt
    24001 ggaccgcatg cgcacggcca gcccggagaa ttaccggcgg gtccagatgt tcgcgcaggc
    24061 gtggttcggc tggctgcccg ggctgaagcg gcggccggcg gaataagcgc gcgggcgggg
    24121 ccgccgacgg tgcgggcgcg cggtctttcg aacacgaaaa attgaggata cacggccatg
    24181 acaagttcgg aaatctcgcc ggaggcgagg attgacccca ccgcgcacat cggccggttc
    24241 tgcgtcatcg gggcgcaggc gacggtcggc gcgggctgcg tgatcggcca ccacgtcgtc
    24301 atccacgagg atacggccgt cggcgacaac gtgcggatcg acgacggcgc ggtgctgggg
    24361 aagcgtccca tgcgcgcggc caactcggcg gtgacccggg agcaggatct gcccccctgc
    24421 acgatcgcgg ccggctgcat cattggcacc cacgtcgtca tctaccgggg ggcccgcctc
    24481 ggcgcccagg tgctggtggc ggatctcgcg accatccggg agaacgtgac cgtcggacgg
    24541 cgcacgattg tcggacgcgg cgcggccatc gaaaaccagt gcgagatcgg cgcctactgc
    24601 aagctcgaga cgaacgtgta catcacggcc tactcgaccc tcggcgaccg cgtgttcgtt
    24661 gccccctgcg ccgcgacctc caacgacaat ttcgtggggc gcacgatgga gcggttcaag
    24721 cgtttcaagg gcgtgacggt gaagacggga ggccgcatcg gcgtccacgc gaccatcctc
    24781 cccggcaaga cggtgggggc cgacgccctg gtcggtgccg gcgccctcgt caccgccgac
    24841 gtgcccgacg gcaagatcgt ggtcggcatg ccggccaaag tgttccggga tgttcccgaa
    24901 gagcagctcc tgaagaatca gggctggaaa gatgagtgag gagaacgaac tatggcggta
    24961 ccgctgctgg acgtaaaacg gcaataccag gcgctcaagg cggagatgga cgaggcggtg
    25021 ctgcgtgtcc tggcccaggg gcagttcatt ctcgggcccg aggtggcgca actggagaag
    25081 gcggtggcgg cgctgagcgg cgtccggttc gggatcgggg tcgcctccgg cacggacgcg
    25141 ctcctgctgg ccctgcgggc ctgcggggtg gggccggggg atgaagtgat caccaccgac
    25201 ttttcgtttt tcgccaccgc cggcgtcgtt gcccgcctcg gcgcgcggcc ggtgtttgtc
    25261 gacatcgacc ccgataccta caacatcgac ccggacctga tcgcagcggc cgtcacaccc
    25321 cggaccaagg ccatcatgcc ggtccacctg ttcgggcagc cggccgatat ggatccgatc
    25381 tgtgcactcg cgcggcggca cggtctggcg gtgatcgagg acgccgcgca gtcgatcggc
    25441 gccgcctaca aggggcggcc ggccggatcg ttcggcgact acgggtgcta ctcgttctat
    25501 ccctcgaaga atctcggggc gatcggcgac ggcgggatga tcgtgaccga ccggccggag
    25561 aacgaccagc gcgtgcgcat cctgcgcgtg catggggccg agccgaagta tttccataaa
    25621 ctggtcgggt acaattcgcg gctggcgacc atccaggcgg cggccctcct ggtgaagctg
    25681 ccgcgcctcg aggcctggtc gaaagagcgg gccgcccacg cccgcatcta tgacgaggct
    25741 ttcgccggga tcgaggcgat tcgcaggccg gcggtgaagg agtacacgac gttccatatt
    25801 ttcaatcagt acaccatcgc cgtccccaac cgcgaccgcg tgatggagca gctcaaggcg
    25861 gcgggaatcg gctgcgagat ctactacccg gtgccgttcc acaagcagga gtgtttcgcc
    25921 tacctcggct accggccggg ggatttcccg gtttcgagca aggcggcgga cgaggtgctg
    25981 tcgcttccga tttacagcga aatgaccgag gccgagcagc aggaagtgat cgcggccgtg
    26041 aggcggatcc tcggttagcg cccggcgggg cggtccctac agggcctatg accagcgcgc
    26101 aggcacagga tcaggcggaa cagccgaggc ggcgggaggc ggcggcatga cgggcgggtc
    26161 gatgcagcgg gcggtgttcg gggtggccgg cgtgctcctt ctgtcgcggg tgttcggttt
    26221 cgtgcgggag atggtgatcg cccatcgctt cggcaccagc gccgactacg acctctacct
    26281 gatcgcgatc atgttctcgg ctctcgccta cgggatcctc aactacgccg gatactacct
    26341 cttcgttccg tatctcactc gccggctgca ggcccgcgcc ggggcggagg ggccgccgga
    26401 tttctggccg ctcgtgaaca ccggcgcggc ggctgcactg gcggtgacgc tcctcctggc
    26461 ggcggcggga ccggcgctgc tccgcctgtg ggcggtcgac tacgtaggcg agggattcga
    26521 ccgcatcgcc ttctacagcc gcgtcaccgc cgtcgtagtc gtgctcggcg tggccgaggc
    26581 cttcatgcgc gctttcctga atgtcaagcg ggtctacacc caccctgcgg ccggctacat
    26641 cgtcttcaac ctgttctcca tcctcagcat cgtgctcctg gcgggccggc tcggggtcgg
    26701 cgcgatcgcg ctcggatgga tcggcggcct gctcgcgcag aatgtctggc tggcggcccg
    26761 cctggtgcgc gtgcacgcgt tcggcgggta ccggccgcag atgatcaccg aggagacgaa
    26821 gtatctgatc gcgacggcct cggcgctcat cgtcattgag ctgatcaacc ggagctattt
    26881 cctcatcgac cgctacttcg cgccgcagtt cgggccgggc gtgatctcgg ccctcaacta
    26941 cagccaggtg atcgtgcagc tgcccgattc catcatcggc tgggcgctcg gggcggtcgt
    27001 gttcccgatg ttctcggccc accgggggga ggccgaccgc gcgaaattct ggttcctcta
    27061 ccggcgcacg gtgacggcgg cactggtgat cgcggtcccg atcgccgcgg cgcttttcat
    27121 caccgcgccc gaagtgatct acgtgctcct gcagcgcggg gagttcgatc cgcagtcgac
    27181 agcgacgacc gcggccgtgc tgcggcccta cgtgccgtcg ctgatcgccc tcttcatcgt
    27241 gtcgacctcg atccgggcct gctattccgg cggctgggtg cgggcagtgt tctgggcgac
    27301 ggtgtccgtg ttcgcggtga aggcggcggc cacctggggg ttgtcggccc tgttcggtcc
    27361 cggcgggatc tcggccgcga cctcggcggc cgaggtggtg ttcgccgtgt tgctcctggg
    27421 catcgccgtg cgccggatcg cgggcgaggg gcgggcggca ttcttgggcg tgctcggccg
    27481 catcctggcg gccggcgccg ccgcggccgc gctcgccggt ctcggccagc agttgctgcc
    27541 ggagtgggcg gcggcgggcg ggcgcgggcc ggcgctcatc aaaattgtcc tctcgggcct
    27601 tgccgtggcg gcgctctacg tgctcttcgg gcgcctgctg ggactgcgcc ggcagttcgc
    27661 cgacgtgctc gcccttcgga cgcagcgggc gggggaggcg taggcggtgg gcgcgcgggt
    27721 cgtgatattc gggtggtcgg gctcggtgca cgtacagcgc tgggtgacgg gtctgcaggc
    27781 gcgcggattc gagatgaagg tggtgtccct gggcggcccg ccgctggcgg actgcgagac
    27841 gaccgtgctg ccgcggaccg gtcgctggtc ctatctcacc cacgcggccc gggcggccga
    27901 ggcggcgcgg gcgtttgccc ccgacctggt gcacgcgcac tacgcgacgg ggtttggcta
    27961 ctgggcggtg cggtcgcgcc tccggccgag cctcgtgtcg gtgtggggcg ccgacgtcat
    28021 cgacttcccc gccggctggt tccgggcgcg gctgctgcgt gcggtcctcc gccgcgccac
    28081 ccacctcagc gccaccagtc agttcttgag gagcaaagtg atcgggctcg acggcagcct
    28141 cgcttcccaa acgtcggtca tccctttcgg ggtggcgatc ccggccgagg cggcggcccc
    28201 cccgccggca ccgcccgtgc ggctgtgcta tgtcaagaac ctcaggccga agtacggcgc
    28261 cgacgtgctc ctgcaggcga tggcccgggc gcggaaggaa ctccccgacc ttcgcctcga
    28321 tatcgccggc gagggggaga tgaaggcgcg gctgctggag aagacgaaaa acctgggcct
    28381 cgacgacctc gtggagttcg tcgggtttgt cccgaacgac cgcgtatacg ctttcatccg
    28441 ggagcaccac atcatggtga tgccgtcggt gtgtctggag tcgttcgggg tggcggcgct
    28501 ggaggcggcg gcctgcgggc ggccggtgat cgcttcggcg gtcggcggtg tgcccgaggt
    28561 cgtgcggcgc gaggaaacgg ggatcctcgt gccgccggga gactccgaga agcttgccga
    28621 ggcgattgtc cgtctcggcc gcgacgccgc cctgcgcgaa cggttcgggc ggaacggatg
    28681 ggcgttcgcc aaggagaact acaattggga gctctcgctc gatcggatgt cggaactcta
    28741 cctgcggttg atcgatgaga aaaaaaagag ctgagcgacg ggtgccgctg ttcgacctgg
    28801 cggtgtcgcc ggcggcgcgg cggtatgtca acgacgcact ggcatcgggg tggctgacgg
    28861 ccggcccgaa ggtgaaggcg ttcgaagcgg cggtggcccg cctcaccggt gtgcgccacg
    28921 ccgccgcggt ggccagcggc acccaggggc tgcacctggc cctggcggcg tgcggcggtg
    28981 tgccgggacg cgaggtggtc accacgccct acacgttcgt agcgaccgtg gaggcgatcc
    29041 tcctggcggg cgggaccccc gtgttcgccg acatcgaccc gcacacgctc actatcgatc
    29101 cggatgcggc ggcgcggaag gtggggaaga agactgccgc cgtggttccc gtggacatcg
    29161 ccgggcaccc ctgcgactac ccgcgcctga acgacctctg cggcgcgcac aacttgccgc
    29221 tcgtggccga cgcggcgcat gcgatcgggg gcgcctaccg gcggcgggcg atccccaagc
    29281 tgtgcgacgc cgccgtgtat tcattctatt ccacgaaaaa cctcaccgcc ggcgaggggg
    29341 gcatggcggt gtcgcgccac aaggagctga tcgaccgcat ccgcctgctg gcccgccatg
    29401 ggctcaccag ctcgacctac gagcggaagc tgtccaaccg ctgggagtac gatgcggtcg
    29461 cgttcgggtg gaaagccaac atggcggagc tgagtgcggc ggtcgggctg ggggagatcg
    29521 agaggttcgg ggccaaccag agggcgcggg ccgcggccgc cgcccgttac cgccgccatc
    29581 ttgctgctct cgccgagtac gtggtgcttc cgaccgaggc gcccgactgc acgcacagct
    29641 ggcacctcta catcatcaaa ctccgcaccg aggcgctgcg catcggacgg gaccggttca
    29701 tccgggagat ggcggcgcgc ggcgtcgaat gcggtgtgca ctacaagccg gtctacgagc
    29761 tctcatggta ccgccgcgcc tttgacctct cggcgcggcc tttcccgaac gccgagtggg
    29821 cgtggcggcg ggcggtgacg ctgccgctgt atccgaccct ggcggcggcc gacgtggatt
    29881 acgtctgcgc ctgcatcgcc gagatcgcgg cgcaattccg gcggtaggcg gcccgggccg
    29941 gccgcggccg ggcgcggggc gggggcgcgg caacaaggtc ttgtcaaacg catccggtct
    30001 gatgatattc cggcatgccg gcaaatttgc ccccgcagta ctacgagctc gagcgcgcgt
    30061 acaacgccga gaaggatccg cgcgaaaagc tccgtctcgc gcaggaactc ctgcgcatca
    30121 tgcccaagca caaggggacc gacaagctgc aggccgagat gaaggccaag atctcgcggc
    30181 accgcaaaga gatcgaggcc ggcggcgcgc gcgcgggcgg agcggccaag gccgtggcca
    30241 aagactacat cgaaaaggaa ggggcgggac aggtgatcct catcggcccg cccaacgccg
    30301 gcaagtcctc cctcctcgac gcgctcacga gcgccaaacc ggaaatcggc gactacccct
    30361 acaccacccg cgaaccgcag ccggggatga tgaccttcga gaccgtccag atccagctca
    30421 tcgacacgcc gccgatctcg gtcgagctgt atgagaacta cctcaccggg ttgattcgga
    30481 atgccgacct ggtggtgctg gtatgcgatc ttgaggccag gtcgatgctc gacgatctcc
    30541 gcttcatcat cgccacgctt cgggagaagc ggatcatcct gcagccggaa gtcgccgggg
    30601 agccggacga cccgaaatac tgcggcaaac ggaccgtgct gtgcgcccac aaggagtacg
    30661 aggatgagga cgggtcgaag cgccggcagt tggcgggggc ctatccgggg tatcggattg
    30721 tgacgacgtc gatcatcgac gaggccagtc tcgaggcgtt ccggcgcgcc attttcgagg
    30781 agctgcggat catccgggtg tacaccaagc cggtggggca cgatccggac tatcgggatc
    30841 cgatcatcct gccggcgggc gggacggtgg ccgaggccgc cgcgacgctc cacaaggatt
    30901 ttctgcagaa gctgaaattc gccaaggtgt gggggaaggg gaagttcgac ggccagcggg
    30961 tgcagaacga ctacgcgctg tcggacggcg acatagtgga gtttcacatt tgaaccgggc
    31021 cggggccgcg ccggagcggc ccgctccgaa ggcagggatg accatccttt ttttatatga
    31081 atacaggtac ggcggccgag tatcttatcg caaaaggaga ttccgatgaa agttttaggc
    31141 attgctctgg cagtcattct gatcctggtg ctcgcagtag cgggctggat ctggtcggga
    31201 cgcggcggcc tcatcgatcg ctcgcaggcg attgacgaaa agtgggcaca ggtgcagaac
    31261 gtctatcagc ggcggtttga cctcgtgccg aatctcgtgg ctagcgtgga caatttcatg
    31321 gaacggcagc aggcgacact gaccgaggtg atcgccatgc gccagcgggt gatcgacctc
    31381 acggcctcgg ccgaatcggc cctcggcagc ggcaacccgg ccctgctcga ctcgcttgcg
    31441 ggacagctct cgcggcagct caactcgttt attaatgtgg tcgtcgagcg gtatcccgag
    31501 atcaaggggg accagttgta cgccgacctg atgacgcagc ttgagggaac cgagaaccgg
    31561 atcgcccagg agcgccgtgt gtacaacgag gccgtgcggg agtacaacgt ctatcgccag
    31621 cggggggtca aggccctcat tgccggagcg gtgttcggat ttccctacgc gcgcgagttc
    31681 ttcgccgccc agtcggaagc ccagacggct ccgagcgtgc gcgacgcctt tgagaagaag
    31741 taggcggtcg gcgaggggtg aagaagctta ttccgatagc cgcggctctc gcggcctccg
    31801 ctctggcggc ggccgccacc ctgccgtcgt acaccggccc gatcaacgat caggccggca
    31861 tcctcgataa ccgggccgtc gaacagctcg aggccaaggt cctggcctac cgcgacgctt
    31921 cgggacacga gatcggcgtc ctcattgtgc cgtcgctgga gggccggggg atcgaggact
    31981 acgcgcacga cgtcttccgt gcctggggga tcggcaagaa gggacaggac aacggcgtgc
    32041 tcttcctcgc ggcgatcgag gacaagcggg cgcgggtgga ggtcggctat ggtctggagg
    32101 aggcgctgac cgatctggag gccgggcggc tggtcagccg caattcgccg atggcgcagc
    32161 aattccggca gggggactac gccggagggg tggcggcagt cgtggacggg atcattgaag
    32221 ccatcggcgg ggagtacgcc ccgcccgaga gcgacgagag ccccgccgcc gcgatttttc
    32281 cgctcctggt catggggctg atcctgatcg tggcgcttgc gaacgcggcc agtcggcggg
    32341 ggatcagcgg ccgccggttt ggcgggccgt tcctcggcgg attcggcggc ggattcggcg
    32401 gcggcggcgg cggcggcggt ggattcagct tcggcggcgg atcctccggc ggcggcggcg
    32461 ccagcggagg atggtaggaa ggcggcaagg tatggcgtca ctggtcgaaa aatatttctc
    32521 ggcggccgat tttgcggcca tcgaggcggc ggttcggcag gcggagagcg ccacgagcgg
    32581 tgagatcgcc gtggagttgg cctcgcggtc gcgcaactgg agcggcgagc ggctgctgtg
    32641 cgctctcgcc gtgactttcg tgtgcatggt ggcggcgctg ctggccaccc gccgcgtcga
    32701 ttggggcgtg tactacaaca cgacccagac gctcctctgg ggcggggtcg gatttgtcct
    32761 cgcctatttc ggcggcgggc gggtgctggc gcggacggcg cgccggcgcg ccgcagtctg
    32821 gcgccgggcc ctgagcgcgt ttcaccagat ggtccccgtg cgcggccgca ccggggtgct
    32881 gatcttcgtc tccgtcgagg aggggcaggc cgccgtcatc gccgaccgcg gcatcgccgc
    32941 caaggccccg cccgattact ggggcggacc ctgcggcacg attgtcgccg gcatggccaa
    33001 gggggagcac gccgagggga tcatccgggc gatcgcggcg atcggcgaag aactcgcccg
    33061 ccacttcccg cgcatggacg acgacgtgaa cgaactgccc gaccggccga aggtggacga
    33121 gtagccgggc ggggcgcggc cgccccggcc cgctatttag cttgacaccc tgcgccgcgc
    33181 gcggcaccct tccggtatgg accagccgct gtattacgac ttccgggaca tcctcatcgc
    33241 gccggcccgg gcgctcgccg ccaaacgcat tttcgtcatg acgctgttcc tgtgcctcgc
    33301 cgtcgccatc tacgacgtgt tcgtctacct cgcggcggtg ctcgacggcg cctcgctcga
    33361 gaccttctgg cggtggcacg ggcttttgcc gctcgccggg ctgaatttca gcggcggctt
    33421 ggcgcaggcc gcctgcggac tggggatcgt gctggcgctg ctggcgctca tgacggggtt
    33481 gttcgccgtg agcgcgatcg agatcgaaca attgcggggg aaccggttct tcccgatgtg
    33541 cgaggcgatc cggttcgcgc gccggcggct gggccagctg gcggccggcg aggcggccat
    33601 ggttgccttc ctcctgttca tcctcctgct ctttctgctg ctcgggctgg tcgttcgcat
    33661 cccggccgtc ggggagtggg tgtacgcggc gacctttctc ctgccggtct tcttcatcgc
    33721 catcttcacc gtgttcatcg tcgccgttct ccaaatctcc gtgatcctgc tccccgcggc
    33781 ggccgccgcc gaacgccacg gcgagtcgtt caccgcgatc ctcgggacgt tttcgaccgt
    33841 catccgtcag ccggtccgct ggctggccta caccgcctac gggctggccg cggccaaagc
    33901 ggcgagctgg atctatgcct acttctgcta ccgggcggtg cagttcacgg cgtgggccgc
    33961 gtcgctcacg gccggggaga aggcggaccg gttggtccgc gaggggctgg ggcatctgcc
    34021 ggtgcgctcg ggcccggtcg aggcggcggt cgcgctcgtg ccggggttcg actggcggtt
    34081 ttcgatctcg cagtgggccg aacgggggga cagcgccgcc ggatacgtta tggcggccat
    34141 gctccttgtc atcctcgcct cagttttcgg ctacgccctg gcggtggtgg cgacgacgca
    34201 ggcgcgcgcc tacgccgtca tccggtttct caaggacgac taccggatcg ccgaggaacc
    34261 gccactcttt cttcccgaag cgcgggagga cgagaccggg tcgggcggcg cgggggcgcc
    34321 ggcgggctga gcggcgcggc ttccccgccg cgccatcttt cggcttgact catcgagcca
    34381 taatgtccat attgccgcgt ccgggaacgc cggggcgggg aagaaacgat cgcgtggacc
    34441 ccacaacgat gcggaggatg cagtgacggc agagaagaag attctggtaa ccggggtgac
    34501 cggggcgcag gggggcagcg tggcgaggca cctcctgcgg cgggggcggt tcgcggtgcg
    34561 cgggctgacg cgccatcccg agtcgccgca ggcaaaggac ctggcgcgcc ggggcgtcga
    34621 aatcgccgcc ggcgacatgg gcgacccgca gagtctggtg cgggcgatgg acgggtgctg
    34681 gggcgtgttc ggcgtcacca acttctggga gcacttccac cgcgagttcg aactcgggcg
    34741 caacctgatc cgggcggtcg cggaaaccag gcccgaattc ttcatctaca gctccctgcc
    34801 caatccgcgg aaactcggcc ggggggagta cgacgtgccg caccttgagc tcaaggcgcg
    34861 cctggaagat gagatccgcg ccctgcccct ccggtcggcc ttcacccacg tggcgttcta
    34921 ctacgaaaac tacatgatct tctccatgct ccagcgggcc gaggacgggt ctttgtcctt
    34981 cggatttccg cagggcgaga cgaagctggc gatggtgggc acggaagata ttgggggcgt
    35041 ggtggccccg ctgttcgagc gcccggacga gttcgccggg cggcaggtgg gggtggtggg
    35101 ggacgaccgg ccgccggccg agtacgccca ggcgctcgcg cgggcactcg aggtcccggt
    35161 gagctaccgc cacatcccgc gcgaggagta tgcccaattc ccattcccgg gcgcccagga
    35221 gctggccaac atgttcgaca tgaaccggcg gtacatcacc gagcggacag ccgatctcga
    35281 ggagagccgg aagctctatc ccgggatccg ggacttcgat gcgtgggtgc gggagaaccg
    35341 ggagttcttc gcctcccttc tggaaccggc gcggtgacgc gccggccggg cgccgataaa
    35401 tctttcgcct ccgcacggtg aatatcgtat acttggggcg agtatatcaa acggtctcgg
    35461 tagagaagtt gcggaggttg aggccatgct catctttgcc accattttcg cagtggggtt
    35521 cctgaccctt ctgctcagcg tcattttcgg gcacgatacg gacttccacg tcgacgccga
    35581 cgccggcggg gtcggagccg acggacacgg gccgggagtc atcaacatca aaatcatcgc
    35641 cctcctcctg gtcggattcg gggcggtcgg attcgggttt cgggccacgg cagggtggac
    35701 gatgatgaag tcgtccatgg cgggcctggt gggggcggcg gcggtcggcg ccatcggcta
    35761 cctcattctc cggctgttct acgccagcca ggcgagttcg acgatcgggg acgaggagat
    35821 catcgggtgc gaggcgcgtc tgatcgatgc gatcgcgggc gcggagtacg ggcaggcggc
    35881 gtgtgtcatc cgcggccggg agatcacctt cctggcccgg tcggcggacg gccggtcgat
    35941 cgcccggaac gcaccggtgc ggatcgtggg caagtcgggc ggcgtcgtga ccgtcgcgcc
    36001 cacccaaaac ggctagaata tcaccaacca caggagcgga ggtctcactc atgtcggaga
    36061 tgatacagtt tttcgtgtgg ccgattatcg gcctggcgat tgtcatcgcc ctcttcgtcc
    36121 tggtcaaagc gctgtcgctg ttctacgtgc gcgtccctcc gaacaaggcg gccttcttct
    36181 acggcgcgcg gagcggcaaa aaggggctgc agcgggcgac gcaggcggag gttcccggcg
    36241 tgcccggggt gaccgtgctc ccgcccggga cggtcgtggt cacggggggc gggcggatcc
    36301 gcaagccgat catcgaggat gtccagttcc tcgacctgac cgaaatcacc ctgccgcaga
    36361 tccgggtgga gaacatgccc aacaccgacg gggtgttggt gacggtcgag gcggtagcga
    36421 atatccggtt caaggacgac gcccagtcgc tgttggcggc cggggcccgc ttcctcagca
    36481 tgccgcacga gaaggtgcag agcgtcgccc gcgagaccct cgaggcgaac ctgcgcggcg
    36541 tggtcggcac gctgacggtc gaccagttga tcaaggaccg cgacgcgttc cgccagcagg
    36601 tgctgcggga ggcggcggag gatctcgccc ggctggggat gcagatcgac gtgttcaacc
    36661 cgcagtcgat caccgaccag caggggtaca tcgaggcgct cgggaagaag cgcacggccg
    36721 aggtcaagcg cgacgcgatg atcggcgagg ccgaggccca ggcggaggcg aagaagcgtt
    36781 cgaccgacgc cgagcgggcc gccgaagtgg tcgcccagga gaacgagcgg ctcaagtccg
    36841 aagcccagaa aaacaccaac gtcgccaaac agcagtacgc cgccgaaatc tccaagcaga
    36901 cggcgatcac ggaacaggcc gggccgctgg cgaccgccgg cgaacggcaa aaggtcattg
    36961 tcgccgaggt caagatcgag gaggaacgct cgcgggccca gatcgcggtg gaagagcaga
    37021 acatcctgcg cgaacagaag gcgcaagagg ccgaggtcgt ggtgccggcc aaggccaagg
    37081 cggactcgca ggtcaaggag gccgagggtt accgccaggc gcagatctcg cgggccgacg
    37141 gcgacaagta cgcgacgatc gcgcgggccg acgccgagaa gcacaagcgc tcgcaggagg
    37201 gtctcgggca agccgaggcc gtcaaggcga ccggattggc cgaggccgac gtgatcgagc
    37261 gccaggggct cgcccgggcg gtgtcggtgg ccgagctggc caaggcctac cgccagttca
    37321 acgaggccgc cctcacactc gagatgctca agatcctccc gcaggtgatc gaatcctgcg
    37381 gcgccgtgtt cggagcgatc gccgcgccca tggggaatat cgacaagctc gtggtggttg
    37441 atgccggggg cggcgagggc aaggacggcg cgctcagccg gtttgccaag accgggccgc
    37501 tcttgctgtt ccaactggtg gagcaggcca aggcggccgg gatcgacgtg agcgcactcg
    37561 tgcagcggct cgggatcaaa ctcggtgaaa cgggcgagcc gggcgcggcc ggcgcaccgg
    37621 ccggaggagg cgcaaccgaa tgaaacggcg gcgatttttg aaaaatgccg cgctggccga
    37681 cgacgtcatc tcgtatcggc cggccgagcc ggatccgtcc gacggacccc cgggcgctcc
    37741 gccccgcgag gacggggcgc cggaggaggt cgaggccgat gtcatccggt cggacgggcg
    37801 gccgatgtac cagctcgaca cgcgagctcc cgaagcgctc gtgatccatt gcggcgatcc
    37861 gcgctttcag actgcgttcc ggcggttcgt gaccgaggaa ctcggcatcc gcacctacac
    37921 gccgatcgtc gtcgggggcg gggcgcacgc tttcggcgcg caggtcttcc tgcccaagaa
    37981 tttcaaggtg ctgtgggagc aggtgaagtt cttcacccgg cagcagaacc tccgtcgcgt
    38041 gatcatcatc aaccacgagg actgcctgtg gtatcggtcg atgaaggggt atcacccgac
    38101 ggtggaggtg ccggtgaagg ggaaactcga tctgcgccac gcggcgcaga cgatcgtccg
    38161 ggacttcgcc gggattcacg tggagtcgta ctgggcggca ctcgcggagg accgggtgta
    38221 ctttgtgccg gtggcgccgc gagcggcgga atagggtacc ttgcggagag gggcggcgag
    38281 ccgcgcgggc ggtcgatgac gtgattggcg gccgagcgag tcaatgctgt cggagaacct
    38341 ggtcctccat ctccccacgc ggcgccatgg gtgagctacc cccgcgctcg atggcgtgac
    38401 cctgcaggct cccgtcctcc gtcgaactga tcgagtgctc aatgcctccc ggcaaatcaa
    38461 aagcccgatg gcggggtgag accgccatcg ggctgtgcca caagaaggca gtaggcaagc
    38521 gggtcacttc accggcggct tcccatgccg gatcatcgtc agcacgtaca cgcggctctc
    38581 gttgccccca ggcccgtgcg cacgcccatt aattttcggc acgcaggtcc gaaccttcaa
    38641 attccgagcg gcgatcccgt gccgcggtca ggccggccac ttcagcgtca acgagcctcc
    38701 tcgcggcggc tgggccgggc tcttcatcag cacgaccgtc ttcgaattgg ccccctgggt
    38761 gagcagaatc atcggcctcg gtttcggtat gctcatatgc gcatcatcct ctctgctggg
    38821 ttggcaacgc aggctccgct caagctcatc cccctacttc agcagaacca ttttcttggt
    38881 gtgctgctgg tcgccggcgg cgaggcggta gtagtagacg ccggaggcaa ccgggtatcc
    38941 cgtgcgatcg cggccgtccc atgtcaccgt atgctcgccg gcggcgaggg cgccgtcaac
    39001 cagacgggtc acctcctggc cggcggtgtt gtagacggtc agttcggcgc ggccggcggc
    39061 cggcacactg aaacggatag acgtggtcgg gttgaacgga ttcgggaagt tctggtccag
    39121 cacgtaaccg ccggggagag tctcgtcggg atcgacgtcg gaagctatgc cgtatttggc
    39181 gacgccggtg tcgacattcg agagcggcga gacgttggcc gcgtcatcgt acgccttcag
    39241 ggcgacatag tagacttcgc cgggctgaag gccggtgagg gtgacccgct gctcgaggcc
    39301 ggaggcgaga ggggtgatga cggagggcca gagggtcgcc gccgaccagt tgctctcgga
    39361 tatggcagtg cgggagtagc ggacttcgta cgcggccgcc gtgcccagaa ggccatcgtc
    39421 gcccgtggcc gtccagacaa cgtcgatcgt gctgtcggca tcgccggagg aggcgcgaag
    39481 gtcgacaact gcggccggcg gcgcggcgtc ggcggcggtg attccggaag ggcagtttga
    39541 gatcgtcgac cagttgggca cctcgtcggc cgtcttcagg gcgaagtagt aggtagtggc
    39601 ctcggccagg ccggcgaccg tcagcgtctc ggcggcgccg gccggggaag gcgacggctc
    39661 ggaaaccagc tgcgtggcca tgttccaggt gacgccgttg gagatcggca gagtcgagta
    39721 gcggatgtca taccgggagg ccgtgccgac cgcgccatcg tcgccgggag cggtccagac
    39781 cagcgtgagc gaggtcgtgg tgggggcgac cacgatgagg ttggtgacgg cggccgggga
    39841 ggatgcgtcg cccgctaccg cggtggtccc ggaggcgacg ttcgacaggg ccgaccagtt
    39901 gagcgcctca tcagcggtct tgaccgcaaa atagtacgta gtgccggagt tgaggccggt
    39961 gacggtgaag atctccgccg acccggcggc cgccggcgcc ggctcgccgg tcgcctgcga
    40021 tgccgaggcc caggtggcat tcgtgatcgg ggacagggag taccggacat cgtactgcgc
    40081 ggcgcggcct gacacgccgt catcgccggg ggcggtccag gtgagggtga tcgaattgac
    40141 gccggcggcg gaggcggtca cggcggccgt gaggcgcggg gccgccaccg ccagaagggc
    40201 gagggtaagg agcagcgtgg gtacggggag cgccagaggg gcgcgggtaa gtcgcgacgt
    40261 gttcatctca cctccgaaac gaaagacttg cttgcctgac cgggggagag tatgcaaagt
    40321 ccggaccatc ccgaagtgaa tttgcaagcc aaactgtggc aagactttgc acgcccgata
    40381 gggatcccgg agaggtgggt tgacggattt ttctgtttga ttccagtgca ttgcacagaa
    40441 tgtgcgcagg aagggtgggc ggcagcggaa atcggcatga gattcgctcc atacggtttg
    40501 gaggagaatt aggactaatt ctgggaactt tagaccgagg ctaaaaggag tatttccgcc
    40561 tgcgagatgg atcttttcgc ttgcctaaaa cggttcggga cattatatta caaagtctca
    40621 acaaagcgct ctccggctgc ttatccggcg gcgccgcaga cggacacgga aaaggtcccg
    40681 gcggagccac accgggaatc ggcagacgaa ataaacggcg gggcgccggc agggcaacga
    40741 cgagccaggg acggatcgtc tcagacttcc caagaacgtg agcagctacc gcgcagtggc
    40801 agggtcaagg ttggccgtcg ggggttagaa gttctccggg tgaagagggc ccggactgcg
    40861 gttacggtcc gccgtgtgaa agtaacagac agtaaacgac ttacctagac acaagatcat
    40921 tcaaccaaac cgctttgagt aaggagatgc gaatgcgcaa gatgatgaag gttctggttc
    40981 cggtgttcat gctggcgctg gtggttgccg gctgcagcaa gggtccggtg gagccgcaga
    41041 ccaacatgcc gctgacggcc tcgtatgcgt tgccgatcga tgcggcgctg gagtcggcga
    41101 cgctgcacat ctatgttgtg ggcgcgacga accatccggt cacggcgcac cgggtcaccg
    41161 cggcctggga tgagatgacg gtcacctgga actcgttcgg cgcggccttc gatgcggccg
    41221 tggttgacgg tttcatgacc gatgccggct ggcagacgac cgacgtgacc tcgctggtgc
    41281 aggcctggat gaacgggacc tacgccaact acgggattct gctggacgag gccgacgatc
    41341 agaccccgcg gtcgatttac gcctcgagcg aaagcgctga cttcccgacg gaagatccgt
    41401 ggatcgagtt ctgctacacg atcggcgccg atccgaccgt ctactgcgac acgatcgtga
    41461 tcacggagga cacgtacatt tccgagtacg atccgaacac caatttcggc gagaacatcc
    41521 ttctcttcac cggccggccg taccttgacg ggaccccggt ggagaagcag accctcctgt
    41581 acttcgagct cgaagtgacg ccgccgccgc tctgcgcgat cggcgacttc gtgtggtacg
    41641 acgacaacat gaacggcatc caggacgccg gcgagatggg cgttccgggc gtgaccgtgc
    41701 agttgatgga ttgtgagggg aacgtcctcg acgagatgct gacggacgcg aacgggtact
    41761 acctgttcga cgagctgcag ccgggccact acaacatcca cttcgtgctg ccggccggct
    41821 atgtctggac gctgcaggac cagggcgccg atgatgcggt ggattcggat gtggatgcga
    41881 acggcacgac gatctgcacg gagctggtgg ccggtgaaat cgaccggacc tgggacgccg
    41941 ggatctacat gccggagcag gaaggctgca cgcggacgcc cggttactgg aagaactggt
    42001 ccggattcgg cccgcagccg gacatggtca cccagtacct cccgatctgg ctcggcacgc
    42061 ctatgggcgc caagagcatc cacgtcacca acgcccagac ggcggtggaa ttgctcggcc
    42121 ggacgtgggg aagcagctcg aacggcatcc tcaagctgta ctgccacctg ctggccgcga
    42181 agctgaacgt ggccgcgggg gcgagcgccg atgacctcgg cacggctatc gccgacgccg
    42241 acgctttcct ggcgatgtac aactggatgg attgggactc gctgtcgaaa gcgaacaaga
    42301 acatggtcaa ccgctgggtg ggcctgctcg acgactacaa caacggctgc attggccccg
    42361 gccattgcga caatgatccg tacgcggcgc cctgcgactg agcaggaacc cgtaatcacg
    42421 gaatcgacaa gcccgcggac cccggtgtcc gcgggctttc ttttttggcc ggcgggcggt
    42481 cccggccggg agcggcgggc cgagggccga aatagaccga tttatccgca aatttgcctt
    42541 gaccggcggg cgcgggaaca ctatcgtaat ccatacatca ggcattgcga atacgtaggt
    42601 gcggtttttc ggcggccggc gaggcgccgg gaaggcgaga ggccgcgcag attgaaggtg
    42661 aagcagggcg tccggaaggt gcagtcatcg tcacagagtt ctcgcgattg aaggtcttat
    42721 agacaagtgt actttggatc acgtttcgac caggcgccgc gaggcgtcgg gagaaaggag
    42781 gagggtgccg cagcgtagaa caacgccaag tgcccgcgag acgggcccat aatgctatca
    42841 gacacgcaac agggaaatag agagtactgg tcaccagttg ttcaggggag tatgaatcat
    42901 gaagagattt gttcttggca gtgtggcgct cttggcggcg gccgcggcgg tgtttgcggc
    42961 ccagataatc ggcccgccgg acgatcagtt cgacatgagc aggattacgc cggggccgct
    43021 cgtggcgtcg acttttgccg ctgacacggc cgtaaatatc acgctcgatc cctccagcta
    43081 cccgatcatc gagatgtacg tggacgtgct ggacaattcg gggtacccaa ttccggggct
    43141 gacggcgggg gatttctgcg tgacgcagga cggagcgccg gtccccttcc aggtaacgga
    43201 ggtcggatcg caggggtgcc cgaccgcgat ctgccttgtg attgaccaga gcgggagcat
    43261 ggcatcgtac ggggcaatgg agttggcgaa aaatgcagcc cggcagttcg tgcgcaacat
    43321 ggacgctttt gaccgggtgg cgctcgtgcc cttctcgagc tgcacgtcgg tgctgcagga
    43381 ttttaccagc gacacgacgc tgctgctttc gcgtatcacc agcctgatcg cctccggttc
    43441 gaccgccctg ttcgacggta tctggctggg cgaggatatc actgctcccc agcagggcgc
    43501 ccgcgctgtg atttcattca ccgacggcgg tgagaataac agcgacgtct gcgggtaccc
    43561 gcccgacgga agctactacg acggcaccta tgccgacgat tcagtgatca tcgtgaacaa
    43621 ggcgctggcg gccgggatcc cgctgtacgg gatcacggtg ggctccgggg cgtgggcgga
    43681 tccgctgatc gcctttgccg gcggatccgg cggatactac atgcacgcac cttcggccgc
    43741 ggatatcgac tcgctttact cgcggatcaa gacaacgctg tgcagccgtt acctgatcac
    43801 gttcacgagt ccggacacgg taatggacgg cacgcagcac caggtcgtgg tctgccaggg
    43861 cgggactctg tgcacaccct gcgatacggg ctggtacaac gagccctgtc cgccggtgat
    43921 cgtgcggacg caggcgacga tcgacctgga agcggtctgc cagccggcgg cgacggggct
    43981 gtccgtttgt gcgctcgtcg ccgacacctg cgctccgttc ctgagcaacg tgacgctgtt
    44041 ctggcgctat gccaacccgg cggtgggtcc gtacaactcg gcggccatgg tcagcgccgg
    44101 cggaaatgag tactgctaca ccattccggg cggtttgctg cccctcggca cgtcggggat
    44161 cgactactac gtgacggcct cggacggcga ggcgacggtg tcggtgcccg cggcaaaccc
    44221 gcagaactac ccgtacgcgg tggcgatctg tcccaactac gccccggaga tcaccggcgt
    44281 cgcggtcgac tgcgaggcgg gcggcgatgc cgtggtggcg gcgctgatca ccgataccct
    44341 cgactacgtt gaccacgcgg tgctgttcta ccgccaggcg ggggatatcc tgtacgacag
    44401 tgtgctgatg gcgaacaccg gcgggtcgaa ttgggagggc gtgatcccat cggaagtgac
    44461 cgcgggatgg ttcgactacg tcgtgcgggc ctgggacaat ttcggcgtga tggcgcagta
    44521 cggaccgctg accgattcgt gcggcgacgg cgacgagccg gtgccgacca acgagtggat
    44581 caacgtgtac tgcggctatc cgctgttgaa cggcgtgccg ctttcgccgg gggatgtgat
    44641 ccgggcgtat gatccggacg gggtgctgtg cgggatggac gtcgtgaggc cggacggatc
    44701 gttcggattc atgccgatct accgcgagga gccgtacaat ccgggcgacc agggggcgga
    44761 gccgagcgac ctgatcacgt tcttcatcaa cggcgttgag gcgcaggccg acccggaggt
    44821 ctactggacc gccaacggcg acggattcga ggtgtgcgcg ttcaccacct gccagacgtt
    44881 cacgctcgat gccgggtggc acctgatttc gtggaaccgg gagtggagcg ggtcgatcga
    44941 ggactttttc gcgctgttgg gaaagccggg gccgtgcatc gaagtgatcc tcgggttcga
    45001 ccggggggcg cagacgtacg atccggacct tccggaattc tcgacgctcc acggtctcga
    45061 ctaccacttc gggtactggt tcctgctgaa ctgcccgatc acgttcgaca tttgcggacc
    45121 gcgggtggat ccgaccgatt tcatcacgat gtggccggac tggaacctgg tgtcgtactg
    45181 gccgacggag gagctggcgc cgccggacgc cctggcctcg gtctacggtt gcctgatggt
    45241 ggcggtcggg tttgacggcg gcggtttgat acacgtgccg ggggatgatc cgtacaatac
    45301 gctcgcgacc atgggtccgg ggttcggcta ctggctgaaa ggtgagtgcg ggggtacgct
    45361 gctctatccc ggctggacgg gcccggtgcc gccgctcctg ccgcggacgg cggcggcggc
    45421 gcccgaggtg ccgccctcgc ggacgtggtt gtcgctctat gggtcgaacc tgaccctgga
    45481 cggcgcggcg ctcggggacg cggtcttcga agtgttcgga gcgagcgggg tgcgctgcgg
    45541 cgtcggccgg tacagcgacg gtctgctgcg gttcacgccg gtgtacgggt atgacgcgac
    45601 gagcgcgacg acggtgcagt acccgaaagc gggggagagg ttgacgctga cggtcaacgg
    45661 ggagcgggtg gcgacaccgc tggtgtggac ggaccagggc gaccgggtgc ggctgagtgc
    45721 gctgacgcgg gaagcaggcg gcgcgctgcc gctgtcctac gggttggcgc agaactatcc
    45781 gaacccgttc aacccgacga cggcgatcag cttcacgttg gcggccgccg gtccggtgaa
    45841 gctgaaggtg atcaacctgc tggggcagac ggtggcgacg ctggtcgacg gtccgctggc
    45901 gcagggttcg cacacggtga cgtgggatgc gagcgcgatg gcgtcgggga cgtatttcta
    45961 tcgtctgacg gcgggagcgt tcacccagac gaagaagatg gtgctgctga agtagggact
    46021 tcgcagcgcc ccagttcgaa acgtacggca gccaccctcg agccatcgag ggtggctgcg
    46081 ttgtttaggc cggcggcgcg ggcgaaccga cgggaatccc ctcgaggtag tcctcgcgcg
    46141 cggcggtgac ggccacgggc acgagcgccc gctcccggta ggcggaagcg gggagtttca
    46201 cccgaagata gttgtcggag agggcaaagt aggcgccgtc gggctcgctt ctgtgctcgg
    46261 cgatgacctc cagcgtccgg ccgacctggc gttcgaggtt ggcgcgctgg agccgccgcg
    46321 agaggtcggc gagaatccgg ttgcggcggc ggacttccgc ggccggaacc ttgccctcca
    46381 aggcggccgc ggccgtgccg ggacggtcgc tgtagctgaa cacgtggagg tagtcgatca
    46441 aaccggaggc ggccaggcgg cgcgtctgct cgaagtgctc ctcggtctcg ccggggaatc
    46501 cgacgatgac gtcggcgccg atggtggtgc cggggacggc cgctttgagg cgcgaggcgc
    46561 gctggacgta aatggcctgg ttgtaagggc ggcgcatggc gcagagaatt tcgcttgagg
    46621 cggactggag ggggagatgg aagtggcggc agaggcggcc gcgcgattcg gcaaagacct
    46681 cgatgaggtc gtccttgacc gcctgcggct cgaccgatga caggcgcagg cgggcgagat
    46741 cggtctcggc gaggaggaac cggcagaggc cggcaagcga acggatctgc ggttcgcgcc
    46801 cccggtcgcg gtagtagccg atgttgacgc cggtgaggac gatctcgcgg tagccctggg
    46861 cggcaagggc gttgatttcg gcggcgatgt cggcggcggg acggttggcc agccggccgc
    46921 ggacgatggc gacgatgcag aaagagcacc actgattgca gccgtccgat attttcaccc
    46981 aggcgcggtt gcgattgtgg aaatcctcga tgaaggggga gcaggagagg tcgggcgcct
    47041 cgcggaacag gtcgggcaga cgggcgggaa ggatgcggtc gatggcgtct ttgtcgcggt
    47101 tggggacgat gacgtcaact ccttcaaggc cggcgatcac ctcggggtcg tggtcgacgt
    47161 agcagccgac gacgacgatg cggccgtggg gattgcggcg ggcggcctgg cggatgaggc
    47221 ggcggcagtc ggcatcggcg cgatgggtga cggtgcaggt gttgatgacg gtgaggtcgg
    47281 ccggctcgcc gtcagcggcc cgccgaaaac cgaacgggta gagcttggcc gccatccgct
    47341 cggtttcata ctggttgagg cggcagccga ccgtctggag gcggatgact tttgttttcc
    47401 tcggggcgtc gacaggctcg gtcatagcac actattatac gcgatttggc gcgggggcgg
    47461 ggagaaaaat gcgggccggg gaagcgtccg cgacccgacc gccgcggggc gcgcgcggcg
    47521 ccgcggaaac gggaaatcct ccttgcccgc cgcgcggttt ggggcgttat tacgcggcga
    47581 tggacaaggc ggataaggcg ccgaccgcac cggtcctggc gctcgtgggt atgccggggt
    47641 cgggaaagtc gaccgccgcg gagatgctgg ccgcgcgggg gtgggcgacg atccggttcg
    47701 ggcaggtgac gatcgacgaa atcgcggcgc gcgggctgac ggtcgatgag cgcaacgagc
    47761 gggcgatccg cgaggaactg cggcgcgtgc acggtccgga cgcctacgcc cggctggcca
    47821 tgccgaagat cctcgcagcc cgggagaccg ggagagtgct gatcgacggg ctgtactcgt
    47881 ggtcggagta caagtacctc cacgaggcac tggccagccg gatgctcgtg ctggcgatca
    47941 cctcggcccg caggatccgg tacgcccggc tggccggccg cagcgagcgg ccgctcacgg
    48001 ccgaggaggc gcgccggcgg gatttcgccg aggtcgagaa cctcgaaaaa ggcgggccga
    48061 tcgccatggc cgactacact atcgtcaacg acgccgcgct cgacgacctc cggcgggagg
    48121 ttgaccgggc catcgcgttt ctcttcccct cctgacatga tggacgagcg ggcgatcaaa
    48181 aagctgctgg cccaggagcg gcagcgctgg ttcgggtggg ggcacgagga ctggcgcgaa
    48241 atgatcgagg cgaccccgga tgacgaagat ggggagctct tcttctacct cgcgcccttc
    48301 ggcggcgagg cctttccggt cgaggtcgtc tgtttccccg acccggacgg ggcgatggag
    48361 atctcgctca ttgtgaccat cgaggagaac gggcgggagg cggaggcggc ttggatggtg
    48421 tacgccgacg gggagaaaga aatgctggtg gagacgaccg accggcggga gggggacgag
    48481 gagggtgatg agtaaggggc cgcagacggg gcggctggcg ccgcccgata tcaaccgcct
    48541 cctccaggag gaacggctgc ggtgggcgcg ccgcccgtac gagtttctcc gcacggttcc
    48601 gtcgcacccc gaggggccgc tcgcctacta ccggttccga tcggggcgga cg
//
LOCUS       DAOPOE010000013        46512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1011_length_46512_cov_5.887164, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000013 DAOPOE010000000
VERSION     DAOPOE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 46512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46512
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_46512_cov_5.887164"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="PKM94_03275"
     CDS             <1..1562
                     /locus_tag="PKM94_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65689.1"
                     /translation="YRLALSVDGRLWHESVFDSAYFETNTAVSLEYDLLEAVAQRKHV
                     RRLYREPGNDYAGCRPHGPHGGLIGCATGLAPGPHDLAITAEDAFGNRSELRFRVVLG
                     PGEFLYGLDSTVRVAADTTDFYFTASEALATVAVDSIVLYVGRGDRWGWTPDGTIERL
                     DGGRLKVRAGGYKTRVTPLRLYLFTEEGGVIADGVFNGLRDDDTGGKFETSHELTEDG
                     LLIKITSLESSAYLSRVDLFYRGKLLGTEYGRMLTPKRYVCLVRPQAKYARIDRYQAV
                     LSHDPARAGRWSDSLNIVAAGIDSTVRAAFDQNAWLDLPKESFFAPVFLELKRNAVVD
                     RSPLALNSDHYLVLPETFPTRTRFAITYHIPYDVEPNRYSGLCWLDKNTNRWVWLENS
                     FDDDRDVLTATAGGGGSFGAVFDYETPRISNLSLREGGVYQNRRPAVNFVLEDTLSGI
                     ADDRNIVIELDGEWLIPEYEPETGRVHSRPLAPLAPGEHYLSIEATDRVGNKTGKYLR
                     FRVAKAGGGDR"
     gene            1626..2309
                     /locus_tag="PKM94_03280"
     CDS             1626..2309
                     /locus_tag="PKM94_03280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD65690.1"
                     /translation="MTVGLIAANTLVFLYSIMGSPRAFQLFIVQYGFIPYELVHFTEL
                     TPQVAAPVWLTMFSSMFMHGGWMHLLGNMLFLWIYGNNIEDYFGPVRFILFYLLSGLS
                     AIFLFTLFGPNSQTPLVGASGAISGVMGAYLVLHPRARVTVLIIFFFIQFVVLPAKMV
                     IGLWFVYQLLLSVVDAIQPSGASGGVAYLAHVGGFVFGYLLLRGLLRFRGPRGRADDG
                     QRIYRVRWD"
     gene            2368..2811
                     /locus_tag="PKM94_03285"
     CDS             2368..2811
                     /locus_tag="PKM94_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65691.1"
                     /translation="MPCKDITDTVKLTVAPDDRVVDYRLFKRTCGGTVGEDKLIGPWL
                     FRRTAAQVAATRNEDFLAKLRAGSDITEYLAVKHFAAVRAALQAALGLAAGRVGDYCT
                     IEAVEYGPEGMAITAHLAVKGLTDEIAACRNCCGRNRSGEETFGV"
     gene            complement(2867..3607)
                     /locus_tag="PKM94_03290"
     CDS             complement(2867..3607)
                     /locus_tag="PKM94_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65692.1"
                     /translation="MLSMRRLSSAVAVVICIFLLSAAGARSAPGDSLLLPSRTVYGGF
                     GVQPVVAKLVAPIRGATIPIKIPAGPEVVELSTEGLVTENWNIALLEIKPDSGFLFIM
                     LANTAGLTIPAGQTPLCNIRYLTGNNSCAGSFSFRWDTALAGSVARRLTYASLDNRTI
                     YPGFNYFRDRVDVLPVVPGDLTSDGAVSIPDLTYFVAYLFRNGPPPGSLNAADVNGDC
                     RGPDVADLTAMIAAFFQAGPPLQCGCVV"
     gene            4104..4460
                     /locus_tag="PKM94_03295"
     CDS             4104..4460
                     /locus_tag="PKM94_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD65693.1"
                     /translation="MDNLRRIMADRRLTINELACRSGVHHVMIRGYLQESDRGKLPSI
                     ETLVALARALHCDLEALTGHEELRGVVEVPKSSPDAEALYDYFTSLPEGDTMREILQR
                     KIYQMLEDNMKKENGT"
     gene            4457..4834
                     /locus_tag="PKM94_03300"
     CDS             4457..4834
                     /locus_tag="PKM94_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65694.1"
                     /translation="MKGLTIVVFLLVVAGAAYGQRLENPDQRPSLIMGMGYSWMNDRC
                     ETTVAPYVIDAVRLGRLIPTFGASVPVSKHVTMLGSMAWGMRRTVGPELSVPVSEYSS
                     VSVQRYLTTFNIEVAVKVYLDRD"
     gene            4850..5206
                     /locus_tag="PKM94_03305"
     CDS             4850..5206
                     /locus_tag="PKM94_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65695.1"
                     /translation="MKRLVSLLLLLPVMAAAQQNNPDRAEHIELGLGFEQTGTDLLIN
                     SVDYRKFDVAGLKLYANYLDPVSDRLTFITSVEWLPDSQGTGYELFGSTIEESTRRFS
                     IMFGLRMYFSGESDRP"
     gene            5507..7777
                     /locus_tag="PKM94_03310"
     CDS             5507..7777
                     /locus_tag="PKM94_03310"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOD65696.1"
                     /translation="MITRQEALDYHSRPRTGKIEVTPTKPCRTQLDLSLAYTPGVAQP
                     CLEIHENPEDVYKYTAKGNLVAVVSNGTAVLGLGNIGAAAGKPVMEGKGVLFKRFADV
                     DVFDIELNTEDPDEVIKCVQLLEPTFGGINLEDIKGPECFYIEETLKRTMKIPVFHDD
                     QHGTAIISAAALLNALELVKKDIDKIKVVYSGAGAAGIACAKLYCSLGVKHENLLMVD
                     SKGVLYEGRGDKWNKYKGEFVRKTDARTLADAMVDADVFVGVSTKDLVTKEMVKSMAK
                     DPIIFAMANPDPEITYPEAMDARKDVIMATGRSDYPNQVNNVLGFPFIFRGALDVAAT
                     AINEEMKIAATHALATLARQNVPESVAKAYGVDHFSFGREYLIPKPFDSRILIWESSA
                     VAKAAMETGVARNPMDIEEYKKLLSERIGQGRKVMTFISEKARRSPKKIVFPEGHSSR
                     ILRAARIVKDEGIALPIVLGDPADIKKIAAEHEVDLLGIEIIDPATSPRRPAYAQAYW
                     KRRARRGITEDKALWLMRERTYFGMMMLATGDADAVLSGVLSDYPSTIRPALEIIDLA
                     PGATRVSGLFAMLQKDRVYMFADTTVNINPSPEELAEIALLSADVARRFGIEPRIAML
                     SFSNFGSARYPESEKVARATTLLKKMAPDLVVEGELQADAAVMPEMLRKLFPFSKLQE
                     PANVFIFPELNSANIAYKLLQRVGGLDAIGPILMGLSKPVHILHRTLDVNEIVDMAAI
                     AVVDAQAPTGGSARAK"
     gene            7972..9000
                     /locus_tag="PKM94_03315"
     CDS             7972..9000
                     /locus_tag="PKM94_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD65697.1"
                     /translation="MGFFDLLSNDIGIDLGTANTLVYVKNQGIVLNEPSVVAIERTTG
                     KVFAIGAAAKEMTGRTPGTIEAIRPLRDGVIANFQISEKLISDFIRRVVKHKYLMKPR
                     VVICVPSGITEVEKRAVRDSAENAGAREVYLLQEPMAAAIGVGLPVEQPTGSMVIDIG
                     GGTSEIAVIALSGIVNNTSIRVAGDEMNEAITQYLKKNYNLAIGELTAEDIKIKIGSA
                     FALDKEISMEIKGRDLVAGVPKNLNLSSVQVREALSETIDIIVEAVRQALEQTPPELA
                     SDILERGIILTGGGALLRGLDKRLRQETNLPVNVADDPLTCVVRGTGKVLENFTAFQK
                     VLERPRKD"
     gene            9120..9695
                     /locus_tag="PKM94_03320"
     CDS             9120..9695
                     /locus_tag="PKM94_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65698.1"
                     /translation="MAGNDRETIDRLVRQAQNGDRAAFSQLVRRTMRDITALTYRMTG
                     DRDAAQDLAQETFVSAWQHLSGFRREARFENWLYRIAANKALNYLGRGGAGRTVSLED
                     AGGAGAERASDEPAPDRALEQARLRDDISAFMRLLPPRQRLVFELRFYRQLGFAEIAE
                     MTGTAVGTVKTNYREAVGKLRTYAAERGWRP"
     gene            9683..10246
                     /locus_tag="PKM94_03325"
     CDS             9683..10246
                     /locus_tag="PKM94_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65699.1"
                     /translation="MAAMTCAEVRDMLHESLGADELSGEAAAHLAGCPACRALHEELR
                     ATAEGARTAGEFELTDLESARLLARIDGEIERLDEAQRGRTPAWLPWLAPAAAAAALI
                     LAIALTGDRFDPRVAVTSDSPATAGEATAAAEDSLYDDLDEPTVQALLEDVSARPAGE
                     PSNALLDDLTDDEYEYLAKNFDIGDIL"
     gene            10243..10860
                     /locus_tag="PKM94_03330"
     CDS             10243..10860
                     /locus_tag="PKM94_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65700.1"
                     /translation="MRRYASVVLAGGLVLCGGAAQAQGPETGPCPGKNRPGPECAIVH
                     AALYAQAEPPAAPEAPEASGPRRRHLEQFRMLKLLELLDLDDKQEMEFLVEFKALRER
                     QLVLQQERRRLVENLATGLREDTLNDSAIAELVARVLTLRREYALASEQFLDRVAGIL
                     TKAQIGKLVVFEERFEFELLDQVRSFRERQGTPGSGWRRQPGDSD"
     gene            10916..11569
                     /locus_tag="PKM94_03335"
     CDS             10916..11569
                     /locus_tag="PKM94_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65701.1"
                     /translation="MRKAMVVLLAVVAAAALAWAQPAPGPNCPQAPQTPQAPMATGMG
                     AGCGMHGGQGMMGCGKHGGQGMMECGMRGHGRMGRDMGDCGGGAGLAQFRKELDLSND
                     QVERLEQLKLNHRMNMIGARADVERAELRLKELLRKDDASTQEVGQAIDQVAQLRAGI
                     QKQCYSQRQECLSVLTDAQKEKLRQLREERRDERREFRQGQGRDRIEFRDRRFGTSR"
     gene            11764..14031
                     /gene="rnr"
                     /locus_tag="PKM94_03340"
     CDS             11764..14031
                     /gene="rnr"
                     /locus_tag="PKM94_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOD65702.1"
                     /translation="MAQTRKEILEFIRAKSAHPMRMKELARALGIPPEEYREFRGQVK
                     ALVDTGELVKLRRGRIGVATEMDVIVGPISVNRSGVGFVAREGEPTDIMIPATQLSTA
                     LDGDRVMVRLTGHLGDRPTGSVVRVVERTRRNIVGTFQKTPHFFCVRPDNPRIHRDIY
                     IPSSHTAGAREGEKVVCRLTVWDDPYVNPEGEISERIGFPGQPGVDMLTVIKSFDLPE
                     EFPEEVRNEGEKAAARLSEEPEEGRLDLTGECIYTIDPADAKDHDDAVNVTRTPRGYR
                     LGVHIADVSHFVQPGTALDAEGFKRGNSVYLPGMVIPMLPESLSNDVCSLKPNRKRLT
                     FSVFMDFDRAGKMLSWEIRPSAIKSRAKLSYEEVQEFFDKGLPEGAGRRKLERVAENL
                     TTARELARLLSARRFAEGSLDFDLPEAKITLNEKGEVIELGNRVRLESHRLIEEFMLS
                     ANKAVALEMFRSAQAFLYRVHDRPDMEKLEAFSAMVARLGYRFPVSPTTKPGDLARFL
                     ETVKDKPEAEFINELMLRSLKKAVYQRNNVGHFGLAFTHYTHFTSPIRRYPDLLVHRL
                     LRMRRPDGKYSPAAARRIPAVIDHAGKHCSETERIAEAAEREAVKVKQVQYMARHVGD
                     FFGGIISGVTGFGFFVRLDNLGAEGLVRVSTIDDDYYRFDEQRYALIGRRTGRMFRLG
                     DRVEVGVLSVDKVNNEIALFAPGLPAQAKAGSGPKGKRKKKGWAEAQPPKAKTPARTR
                     TAPPGYGRTRKKRPR"
     gene            14050..15462
                     /locus_tag="PKM94_03345"
     CDS             14050..15462
                     /locus_tag="PKM94_03345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65703.1"
                     /translation="MVNRTIILLAPETAVASGIAWERLGETVTELSELYRLVRERRVD
                     LVLVDENRADLKRPVARRIRRQNGLTEIWLLTHDREPAPAEVQFIDGRLPLSLGPDGL
                     AQKIAHIYHGKHLLERYDMVGRAPQMKIVAETIERIAPTDVSVLVVGQSGTGKELVAR
                     ALHTQSARKDRPYVAINCGALAEGVLESELFGHERGAFTGSVARREGLFARAEGGTIF
                     LDEIGETKPDTQVKLLRVLEDGTYYPVGSSTARHADVRVIAATNRDLTEAISERRFRE
                     DLYFRIGVVKIVLPPLLDRKQDIEALLQHFWRGKELDYTDPALDLLLKYDWSGNVRQL
                     KNFADRMRALKPRGTVEVEDVERFIDEQHVTAKHLPVTTGKTVEEAGQELIYRAILQL
                     GAEIRLLRDLITAHLPSEPPEETAEAAPAADGATMEEVEERMIRRTLRETGGNRKEAA
                     RRLGIGERTLYRKLKKYGLS"
     gene            15467..17101
                     /locus_tag="PKM94_03350"
     CDS             15467..17101
                     /locus_tag="PKM94_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65704.1"
                     /translation="MRAVTLTAAITIALAAAGGTAQELSTDVYPSEDELLEALRRGDI
                     DQERYDILREILREGIDQPSRYLFNEIPNLAGEERADTALPRLAAEQAEAFTAKRPPA
                     PRLAVRHRYAQEIERSGRGSYRTTVSGRLGPHLDGAVRLERTVSGRERVVGRMIRWRP
                     ERGVICEAAVGSLARRLGLGTVAGYRGKILGSSPRLDGESVRYPDYGGQNGGYVRALA
                     GRWQAEALVSHTRDGEFTLLSAAVAVARRCGKVRAASIAGINRLRSRVRGEPRSDVKY
                     AGLLEYAYGNGRIAAEAAGQAGVRSAFGAVVFEGRHRLTGAEIRYCGWHYDRDYVDLA
                     GGSAGGTIAARDSIPGPAFAFSSRRTGNTGATVRTEAALTARVALETGVAGSIRSSDT
                     LRGEVLAGLIAAASRALQVRADHLERLTRTRESLYDRDAVVRRTRAEVRYDRAALAVR
                     TYVGYTVRSGAADSWNWFARLRYRSARVGDLEIWANLGTISPARRQIDYAYGYVQLRQ
                     MIGEAVAIAVKIAQRYSRGADDPHRTTVALEAEAVL"
     gene            17231..18205
                     /locus_tag="PKM94_03355"
     CDS             17231..18205
                     /locus_tag="PKM94_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD65705.1"
                     /translation="MRKVRNSAAARSQGAGEIGAPAAAASAAPAQAEVLALAKAMLGA
                     FEGTLGFILAVGALLAGAAAPVQAIRLNEVLANPTAGYGSEWIELENETDTAVSLAGW
                     RIGDALRTAVIIDTGAGPAVSDAAVPPRGYAVLVQDSAAFFASAGRPEAPVIEPPSWP
                     VLNNTGDSIRLIAPGGALHDHFVYRAVFPDNRTWCRHPIDPSRWDRSADPGGTPGREN
                     VLPDSDAAGAAALTVTPRVFSPDGDGVDDSTVIGLEADEELDRRVEIYDREGRRVWEM
                     AIPAGQRQAQCVWWGRSRAGERLPIGIYIIYLEAIGGESARRTVVIAR"
     gene            18202..18768
                     /locus_tag="PKM94_03360"
     CDS             18202..18768
                     /locus_tag="PKM94_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65706.1"
                     /translation="MRRPGRAAIGAGLLLACAAGAAAFELPTPRGEGFGRTMLLADAS
                     AAALTKAPTAGIRTGEWRFELGGVRRFELSDLDQVFAAAAGRLGRAVLAAGVSQFGNT
                     ELYAETTVRLAAGWRVGAFGAGGSLAAKIVEFGGGYGRLHAAAVGLSATYAGKRVQAA
                     AAAENLNCPRLDAAAAPDHRRLDVYVEI"
     gene            19031..20203
                     /locus_tag="PKM94_03365"
     CDS             19031..20203
                     /locus_tag="PKM94_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOD65707.1"
                     /translation="MEPILTREEIEQRERATLAPYAALAAESRGRRFPSRDHPLRTVF
                     QRDRDRVVHAAAFRRLEYKTQVFIPHERDHFRTRLTHTIEVAQIARTLARSLRLNEDL
                     AEAIALAHDLGHTPFGHSGEDVLKDLLAGFGGFNHNRQTLRIVDLLEQRYPEHPGLNL
                     TYEVREGIVKHETVWDHKVAEFDDHAHPTLEAALVDVADELAYNAHDIDDGLSAGMLA
                     FDDLCRQPVWEVFGLDGRGAFERLHEDQKRYALVRRLIDLTATDVIAHTAAQIARFRI
                     ASLADVRSAPEKLTGYSPDLRPAVTALKQFLFSDMYQRPRLLALAKRARTILEGIFDR
                     LMAEPERLPARFREMLQTEETEIVVTDYMAGMTDRYAEKVYRELTGKEPPDLPEML"
     gene            20302..22749
                     /locus_tag="PKM94_03370"
     CDS             20302..22749
                     /locus_tag="PKM94_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOD65708.1"
                     /translation="MASHRMGTAVPALLLLLVLAAAVSAADMQVRVYLDGKEDAAGLH
                     PLGLDVVWQGKDFIEIIATADELRELEGLGLRTEIVHGDVAAFYRSRLDHARDMGGYK
                     TLAEIQAYMDEIAFFHDAIVFPKTVIGYTLEGRPMYAMKISDNPELDEPDEPEVLYTA
                     AIHAREVITPETVLRFMDYLTDNYGTDPAVTDLVNSRELWFVPVVNPDGYYYNQVIEP
                     DGGGMWRKNRRNNGDGSYGIDLNRNYGYEWGYDDDGSSPVPSDATYRGTGPFSEPETQ
                     VMRDFISAHEFVFTLYFHSYSNLVLWPWGYDQIYTPEEPLFRILGDSLAAYNGYEPTP
                     SWGLYVVNGSSDDWGYGEQLLKNKNYAFSIEIGGYSDGFWPDPSRIDQLTLENRGPAL
                     FLARVAGNPYQVMPPGQPAVSVDDTVDASGYSVQWSLADTLNPAVLWELVELQGFGRT
                     TDQANAWDNFSSNGFSISGARYSSAPTSFFSGAANNAARRIQSVETIAVRASDVLTLR
                     VWYDIESGWDYAYVEVSTDGVAFTPIAGTITTNDDPHGYNRGNGITGSSGGWTLASFG
                     LAVYAGQSVYLRVSYETDGSQLGEGIYVDDITPVEGYASETVISSALTDTSFAFTDKP
                     TGTYYYRVRAQDAQGQWGPFSAAVPTVVTDGEYQCVDSDLDGFGDPGYPDNDCPTDNC
                     PATANPDQADGDADGVGDVCDLCPNDAQNDGDADGHCGDVDNCPLIANADQADADLDG
                     VGDACCCALRGDSDGDGAIKVGDLTMLVAFLFRGGAGPGCPAHADVNGDGGPAVSDLT
                     MLVGYLFRGGEPPAACP"
     gene            complement(22850..23965)
                     /locus_tag="PKM94_03375"
     CDS             complement(22850..23965)
                     /locus_tag="PKM94_03375"
                     /inference="COORDINATES: protein motif:HMM:NF023242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HOD65709.1"
                     /translation="MSDSAARLRLGAHRDLAGLVALEERAFAIDRFHEEQIEYLLEEA
                     NATTFVVDLGGRIVGAAYMLWRKGAPVGRLYNIAVDPDMRGRGLGQRLLDACRQEAEW
                     RNCTTLSLEVRVDNAGAIALYRRNHFEAVGRLPKYYEDGADGVRMTRPLTAGRPERLR
                     LKVPYYAQTLTFTCGPAALIMAMKYHRPDLEDDRLLEVTLWREATMIFMTSGMGGTGP
                     FGMAHAVVDRGFSARVLLSKNQTPFFSSVRTADKRRVIKLVHEDLRERAYHRGIPVSY
                     YDFSFDDIAVEMLRGKIPIVLISTYQLHGDRAPHWVVVTGFDREFVYVHDPYEAEYGG
                     DPHRARNLKIDLPLFSRMRRYGRDLYKSVLFVGPGEA"
     gene            complement(23962..24852)
                     /locus_tag="PKM94_03380"
     CDS             complement(23962..24852)
                     /locus_tag="PKM94_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOD65710.1"
                     /translation="MGRIGVYIERYTITRSDEMEALMRFAMVARKIGHEVDYLFRPDV
                     AKIPQYDAIFIRALTDPLNTSYVAARMAEMHGKRVIDDPRSIYICCDKVNMYAHLQRE
                     GVPMPDTLFLEEKDLTRARAAQVFEQLGSPVVLKAPNSSFSMYVEKAETPEAFVRVGN
                     RFLRRSDRLVAQRFIKSDFDWRVGIVAGEVLYVCQYTIPRKRWKILTYAENNRAISGP
                     VKGVPIEKADPQLLEVALAAARAIGDGLYGIDLKQVDDGYAVIEVNDNPTINAGEEDQ
                     AAGDLYEKVIRFLVEGKTTP"
     gene            complement(24840..25472)
                     /locus_tag="PKM94_03385"
     CDS             complement(24840..25472)
                     /locus_tag="PKM94_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HOD65711.1"
                     /translation="MTKNTKRAKANRYVAILNEDDTFVNLAGRYEYLELPYYVSQDYE
                     HSRLPIRPTCKEMLDAYIVPLFLERAKLAGLDVPEYYVTNGYFEAPAIVDSINPFMSR
                     SRTVLKMSQQKSVSKSITRNFTYAACVQEIPPEAQIEHFRAVLGWSVAARYRDSAEKI
                     WRVFQIPLARVRVLRTYDDRILLSKISPLLYRDLKQRELEYLEKKIRWEE"
     gene            25740..26120
                     /locus_tag="PKM94_03390"
     CDS             25740..26120
                     /locus_tag="PKM94_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65712.1"
                     /translation="MDCYFGSIHLPEDRQLDQSVVVSFKIPELGIKFKAPFDAVDANH
                     GDLAALLALLEFIDSNQKYFTRHTYQIYGNNLKVINAVNRRDCLPPEFAPLIAKALGY
                     RDKYRFSLAWAPLRSNPELEELFD"
     gene            26113..27150
                     /gene="holA"
                     /locus_tag="PKM94_03395"
     CDS             26113..27150
                     /gene="holA"
                     /locus_tag="PKM94_03395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOD65713.1"
                     /translation="MIDAHGAVIYVGIAMPSPSELLRDINAGVFKPAYYFYGPEDYRI
                     IEATKYLARQFLPDRQLQTNYRKLSGRKNPAREVMAELANLPMLGERQVFSISDFQSY
                     QPADVERILGMLRPPDSTRVVVFSSPSPRTPKKTSKFFKAVQAAGIVMVEFPRLTAKE
                     VRAQIQTRLQKAGLQIEPEALRVLVDLVAGNLGAVAAETDKLANYRGKGGTVTVDDVT
                     AVAAGYETINIFTLADQIVTEQPHRVLRSLEQLIAEGNSPVTICTLLSTHFLRLYLVR
                     HRKPVPGNMAWLESRLRPQANRFDSARLEQILIDLAEAEAQLRGGGLPPRLALEILVV
                     GLMPGRDALYG"
     gene            27143..27742
                     /locus_tag="PKM94_03400"
     CDS             27143..27742
                     /locus_tag="PKM94_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65714.1"
                     /translation="MGENSREENLPRPVSGLDKAAERALVEAAQNGDNKAFGRLIRLH
                     QKRLFRYIYAMLGSFDAAEDVVQEAFVKAYENLKSFKPEYDFYPWLSTIARNTAYNLV
                     RKEEKNESLNALAEKGFDPESGDPGPLEKLLAEEGEKRFYQALKTLPVQHRVAFVLRH
                     LEGMNYAEIASYLKIPPGTVDSRLYRARQMLMEALQDLL"
     gene            27766..29043
                     /locus_tag="PKM94_03405"
     CDS             27766..29043
                     /locus_tag="PKM94_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD65715.1"
                     /translation="MDHGYFKDRVSGYLDKELPDYEMEALRRHLGECAECRALYEDLR
                     RLEALVERHADLGGDEDYWEAAAARIEKRIGIAGAPEVIALPPRRSRGLAWKLVGVAA
                     SIAALTFIAVNYTDIVRQRDESPKMSFPSEPPSARDSAAAGRAGEPSPAGEEQPAEKA
                     ERTAPPTAEPPEPAEIAALPELKRQAAEDEQLPVIAEEAQEAPRLKPAGQELAASGGK
                     AAAQDAEAEANRSAAAPAAPAAPPPEPVSAETRKDEGAADVRLADRLVLRDLMVAKEP
                     ATAAAPSLSLADSSAADRRGRLPLAQSSKGFAAAGESAPAATLADWRNRRDSLESLWS
                     ELRSEHLNLALPKARRDRAALGDIELVERQLLEAQVQVGRLAEGADAPERDRAIQFFR
                     TYLELADVRYRETARLYLDELAPDTVDTSGSRR"
     gene            29113..29574
                     /locus_tag="PKM94_03410"
     CDS             29113..29574
                     /locus_tag="PKM94_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOD65716.1"
                     /translation="MSYTRLYRSAENKVIAGVCGGLGEYFDVDPNLVRVFAVLLFFAT
                     QGFATLAYAAAWIIVPKRPLEGAVEEVRPERPRSSWIRYLPGLVLILIGSLLLVRQHW
                     HWIDFDQWWPALMIAGGLFLLLRPRRRREACRPDLSAAPGEIPGGNEGRTA"
     gene            29571..30473
                     /locus_tag="PKM94_03415"
     CDS             29571..30473
                     /locus_tag="PKM94_03415"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOD65717.1"
                     /translation="MTPARFRWGLFLVLLGLLLLLRNFGVLNDNFWLDLLILFPVVLI
                     MVGLEKIFARTRLSIISYLTSVALFVGGLAIAFTSSRGGDSTSFFSTATYVKDADSAV
                     RFTRAVLDLDESDLTIRDSGDDLVYAKFDKFTRKPRIDFREEDSLAVVEFASRPHGLL
                     GGAIKIETDEAPDWFVRFKRDVPLEMRLAGVKSDMHLNMSTTPLRALDLSADQSSVYL
                     KIGDLEPFVKVSVFGEDTELRLRVPRTVGLRVLAGEEYKAYLYSLGLITRDDAWVNEG
                     FDTLPNKIEVKLDDRLQSVSVDFF"
     gene            complement(30565..31332)
                     /locus_tag="PKM94_03420"
     CDS             complement(30565..31332)
                     /locus_tag="PKM94_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOD65718.1"
                     /translation="MEETPYERIPVYDPNPEPDHVRRFRAASGNAQFRRTWLTAVALL
                     ALVVIYPAVSIAFAEDPSIALKGLNDTMRILMLLVTIVIQWGLFVVLVLAAESEKTGL
                     AGLGFRRFRPVDLAWAVAFLLAANLLLSGLAWVLGQIGLPMSGDIRFLIPTDLVGRIV
                     WVVVAITAGVVEETAFRGYLMTRLRLLGRTRGWLMPTVVSALVFGSLHAYQGAPGVIV
                     IAVYGALFSLLYIRTGTLWPAVIAHFFQDLSALFFPQ"
     gene            31608..32216
                     /locus_tag="PKM94_03425"
     CDS             31608..32216
                     /locus_tag="PKM94_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65719.1"
                     /translation="MRKSRLIYSNGIVGLGRDPGRPPDPPADTPGPALPEPPEDSAAT
                     RAVTAALAALSDEEREFVRCFYYMGCTVAQIAGRTGRAEHRLEALRRRVERRLRARLG
                     PFVRARYGIGADPGRACPICGAARREAIEQAIAAKRPEETWGPVLRQLRERFGLPLRS
                     PQTILSHVKFHPKPPDRPAADSGTQDTPPTPDMNQPTDGAHR"
     gene            32213..32503
                     /locus_tag="PKM94_03430"
     CDS             32213..32503
                     /locus_tag="PKM94_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65720.1"
                     /translation="MSEMREKPEGSRPPAPEAAARTPRNHTIHVFVAHDLKARLEALA
                     ARYDRTTADMVRAVLRIGIPMMEGLSEAEEVMVKEYIELFRNLRQVKSLRDI"
     gene            32616..34193
                     /locus_tag="PKM94_03435"
     CDS             32616..34193
                     /locus_tag="PKM94_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOD65721.1"
                     /translation="MSERSLGRRAALTALCLALLAGCGSDPAPRPADQVEAALAAAGA
                     NRGELEKVLAHFDGKGDTLMLDAARYLIGNMEGHSYVTYALIDSLGDTIPWDAARFPD
                     YGALQAAYGELEKARPGLDFKSAVAARDLETVTADYLIENVELAFAAWRTKPWARKLS
                     YEEFREGVLPYRGSNEPLEPWRAELMARYADLAGKMTDSTDPVEAARLINDDIKSWFR
                     FDERWYFHPTDQGLAEMKTTGRGRCEDMTNLTIYAMRANGLAVTSDYTPYWANAGNNH
                     AWNALILPGGKAIPFMGAEADPGEYNLIRRAAKVYRKSFARQPRNLAFVKEENRKVPG
                     WLAGKSYRDVTAAYGEVCDVAVTFAEAPADSVRFAYLCVFNDGEWKPIQWAEIAGRRA
                     VFAAMAPEVVYLPGFYLNEEIVPAGEPFLLEPGGVQQALTAELAEPVDIVCRAIMSKD
                     LDKASENASGSRLMAGVDYELFFWGGEWKSLGTATAAEEPLVFLAPSGGLYRLAAAGS
                     DGDERIFTWREGAQTWW"
     gene            34301..35791
                     /locus_tag="PKM94_03440"
     CDS             34301..35791
                     /locus_tag="PKM94_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOD65722.1"
                     /translation="MGEPAGTRLDVDGIMNRAAAAAEAFRRCDQEHTDRIVTAVYRAG
                     FDQRIPLAKMAAEETGLGVWQDKVIKNVIATRFVYENIKTLKTVGVIAENEEAGIVEI
                     AQPIGPIFAVTPITNPTSTVLFKILIALKARNPIVIRPHGAARKCSIEAARICYEAAL
                     AAGAPEHCVQWVRRSTEEETLALTRHRKTALILATGSEALVRAAYSSGNPAIGVGPGN
                     VPVFIGKTADVPFAVDQIVMSKTLDHGTVCASEQALVVRACHAAETRRLLIERGAYFL
                     TPEEIRRLETVAFNPATRVMRVEVIGQPAEKIARMAGIEAPAGTRLLVAELEDVGPHT
                     PLSLEILAPVLAFYVAEDIDQAIALCRKINHNGGLGHTISMFSNNEERIRYFASTMNA
                     GRILVNTPASQGVLGGTYNALQPSLTLGCGTGGKNITTDNISARHLLNIQRIARRRVS
                     PCIGPEMMAAYLDETVDALALERRCAEMEAGAARPGDPAGEPPKKT"
     gene            35846..37237
                     /locus_tag="PKM94_03445"
     CDS             35846..37237
                     /locus_tag="PKM94_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOD65723.1"
                     /translation="MYEKQRTIRRPISLSGIGLHTGNLCTMTFHPAPPDHGIRFVRVD
                     LPDRPSVIADIDHVVDISRGTTLQDGEAKVHTVEHVLAAFAGLQLDNMLVELDNNEPP
                     IGDGSAKPYVDKILEAGIESQDADKVYLEIDTPMAYNERERGVDLVVAPSNDLRITFL
                     IDYKNPALGTQYTTLQDLEQEFVDEFAPARTFCFLSEVESLKAAGLIKGGSLDNAVVI
                     YDSDRGQVEVDRIRRALDLKEEAFVGKTGIINDIPLRFYNEPVRHKALDLLGDLFLIG
                     VPFKGHVLAARSGHKANVALARKMRDLYKKKKIASRYAGRASAALFDIDAIQRIMPHR
                     YPFLLIDRILDLEPDTRVSALKNVTINEPFFQGHFPGHPIMPGVLILEAMAQAGGVLL
                     LNAIPQPETKVVYFMSIDNAKFRKPVVPGDQLRFELQMISFRRMTCKMAGEAYVDGEL
                     VASAEFLAMVRDR"
     gene            37234..38007
                     /gene="lpxA"
                     /locus_tag="PKM94_03450"
     CDS             37234..38007
                     /gene="lpxA"
                     /locus_tag="PKM94_03450"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOD65724.1"
                     /translation="MSGIHPTAIISPAAELAEDVSVGPYTVIEDDVRIGPGTAIASSC
                     LIASGARIGERCVIAHGAVIATQPQDLKFGGEKTFVEIGDGTLIREYATVNRGTAAHG
                     TTTVGKGCMLMAYSHVAHDCILGDHVILANAVNLAGHVEIDDYVIIGGIVPVHQFVKI
                     GAHAMIGGGFRVPQDVCPYSLCGGYPLRVMGINAVGLRRRGFTRDAVAAIEQAFKILF
                     FSKLNTSQALERIRAEMEITPEVGRILEFADRSTRGLTK"
     gene            38043..38510
                     /locus_tag="PKM94_03455"
     CDS             38043..38510
                     /locus_tag="PKM94_03455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD65725.1"
                     /translation="MSQTAVVTFRAAKETEYAGFLDLIHQHAADYLEPSLTLLGLDKT
                     EFAHLFRTRGQVVSILADRAVAGFYWIEERDGTLHIHGLVIKPEFQKRGLAAAALAHL
                     ETGASPDVARFELGVHRANGRARKIYERAGYRVTRALPDFDFSIMHKDRPAQS"
     gene            38673..39662
                     /locus_tag="PKM94_03460"
     CDS             38673..39662
                     /locus_tag="PKM94_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD65726.1"
                     /translation="MTRIRTGVVGVGALGRHHLRWFSQIPESHLVGFHDIDPEKRERY
                     AAEYGVAAFATVEELAEAVDAASVAVPTTAHRAAAGALLKRGVHCLIEKPVTSTLDEA
                     HELAELAAASGARVAVGHIERFNPAVQAVLRIGVRPAFIEAHRLAAFDPRGTDVAVVR
                     DLMIHDIDLVLTLTGARVVDIQASAVAVVSDQPDIANARLTLDSGAVANLTASRISLN
                     QMRKLRIFQRSGYFSLDLAKKQADLYRLAAPGEEIEGLRVPLGKSGRELIYASHGDTG
                     QDQLQMELSAFLRALLDGGAMAVPLAQGIEALRVALEVERTGRASLARMLADG"
     gene            39673..40785
                     /gene="lpxB"
                     /locus_tag="PKM94_03465"
     CDS             39673..40785
                     /gene="lpxB"
                     /locus_tag="PKM94_03465"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOD65727.1"
                     /translation="MELFLSAGDLSGDNAAARLVAALRTHDPGLRLCGLGRTRLARLG
                     QEQFAPGGDLAVIGFWEVAKRLPYFRRLLARCADEIARRAPRAVILVDYPGFNLRLAR
                     RVRRLGIPIIYYISPQIWAWGSKRLREIEQLVDLMLVILPFEEDFYRGSRVNVRFVGH
                     YLLEDIAAEMIASPPPERMAGGLALLPGSRPQEIDRLLGPMLETARRFTRAHGTRAVV
                     AALAGAYPYDAALRAEDHAAITVAYDDARRVIHDSALVLTASGTATLEAAVIGRPMVV
                     AYRTGEITYRIARRLITLDKIALVNLVAGQEVVPELVQHEATPDRMTAALERLWNDRE
                     LYLRTAAALGRIPGLLGGTGASDRAARLIIDHLEGK"
     gene            40790..42028
                     /locus_tag="PKM94_03470"
     CDS             40790..42028
                     /locus_tag="PKM94_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOD65728.1"
                     /translation="MLSLYRMFTRFIHTVSYPVTRFRAARGEQIWRDRLGLDTAGIEA
                     ELWLHAASVGEVKVLSCLYRYLKRRRPHLRACVTVTTTPGHQTARKLFGEEAAVRYLP
                     ADTTPIVARVLAAVRPRCLVIAETEIWPILITQAARRRIPVILVNGRMSDTGFRRYRM
                     VAPFLRGLLPCYDRFFFKSEQDAERYRYFGLPESACRVTGDMKFDAPLWPRSAGRRAA
                     VRATVGARDHDWLIVAGSTRPGEEALLLRGIPDLRGAGNGLRLVLAPRHVERAEEVAQ
                     ACRDAAVPCAVYGSADLSAPVVIVDRMGLLMDLYMAADLAFVGGTLVDVGGHNILEPV
                     WAGTPVLYGPSLANVGDAAEYIEAHNYGARVGGPEEFAAAVRDIRAGRRTFATKTEHD
                     YDRSPTAAAGEYILAKLSDD"
     gene            42021..43109
                     /gene="lpxK"
                     /locus_tag="PKM94_03475"
     CDS             42021..43109
                     /gene="lpxK"
                     /locus_tag="PKM94_03475"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOD65729.1"
                     /translation="MIERCWKRFLRRSGMSPWGLLGPPLWLASLLYRFVPGLHRRLTK
                     ERVRLSVPVIAVGNITVGGTGKTPMVHLLARRLIDEGFRIGIVSAGWGRAGDEPILDE
                     GTGLQDRSADDVGDEVLLLAHLVPQAVFSIHPSKSEGARRLAACGERLDAVIVDDAFQ
                     HYRLARDLDLVTYDAALPARVLRPFPYGVMREPRSALRRADIVVITRANFARDIGRLS
                     RRLCKISPRAAHYTAQFSISGLVGAERRYPVKYLEDKSVFLFAGVGNFEPLRRQVSVL
                     AGDLDAALELSDHQRYDAATLARIKRLADRHDSDVIVTTGKDWVKIRHFDFGRELYYL
                     AQSVDLDPGEEQLIAAIEDRLNLRKAGH"
     gene            43109..44710
                     /gene="nadB"
                     /locus_tag="PKM94_03480"
     CDS             43109..44710
                     /gene="nadB"
                     /locus_tag="PKM94_03480"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOD65730.1"
                     /translation="MERHYDFLVIGSGIAGLFYALEVLERHPQARLALVTKKRESDSS
                     TNRAQGGIAAVLSGADSFESHVADTLEAGAGLCRRDVVETIVREAPDAIRDLVEIGVR
                     FTRTAEGYALGREGGHRAARVVHADDLTGQEIERALLAAIKERRGQIDVYRDHIALDL
                     IKGTADGTTVCAGAYVFSEKDHSFYAFYAPVTLLATGGLGQVYFHTSNPKIATGDGVA
                     MAYRAGVPVGNLEFIQFHPTILYSPGRWPFLISEAVRGEGGVLKAVDGRLIMDGAHEL
                     KDLAPRDIVARAIDKELKQSGEEYVLLDISHRDAAFIKKRFPNIYKQCLQRGFDLTRR
                     PIPVVPAAHYACGGVVSRVSGETALAGLYVAGEVAMTGMHGANRLASNSLLEAVVMAK
                     RAAAAACAYAEAAPPAPHAAITPDFYSSLQYPREKVLLAHDRSRLTRVMWDFVGIVRT
                     ENRLRLALEEVQRVKNAVEQYYLATPATYQVVELRNMATVAELIVRSALLRKESRGLH
                     YIEDYPAPAPGAPTDTIITNPHAQG"
     gene            44735..46258
                     /gene="guaA"
                     /locus_tag="PKM94_03485"
     CDS             44735..46258
                     /gene="guaA"
                     /locus_tag="PKM94_03485"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOD65731.1"
                     /translation="MILILDFGSQYTQIIARRVREAHVYCEIVPYNHDLGAYRGRNVR
                     GYILSGGPASLSDPGSPRLAPEFYDTNLPVLGICYGLQLLAERFGGRLVASSTREYGR
                     SRLSVVEADDLLAGIPATSNVWMSHGDSIAVMPPGFRIIGSTETLAAAAIAWPERKLY
                     GVQFHPEVHHTDEGRRILDNFLFDVCGVKGDWTTEAFIDSAIARIRAQVGDGNVVLGI
                     SGGVDSTVAAMLLQRAIGGRLHAVFVNNGLLRKNEFEDVTAMLRTLDINLRPVDASAE
                     FLARLAGVIDPEAKRKIIGNTFIDVFEAAARNIGNVGFLAQGTLYPDVIESTSFRGPS
                     ATIKSHHNVGGLKERMHLKLVEPLRELFKDEVRRLGRTLGLPEQFIRRHPFPGPGLAV
                     RILGEVTKERCDLLREVDAIFIEELHNFNIYDDIWQAFAVLLLIKAVGVMGDERTYED
                     VIALRGVTSVDGMTADWARIDPDILAHISNRIIRHVKGINRVVYDISSKPPATIEWE"
     gene            46295..>46512
                     /locus_tag="PKM94_03490"
     CDS             46295..>46512
                     /locus_tag="PKM94_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65732.1"
                     /translation="MVRMLSWLAVILIGAFVAVNIGLYFAQGRIVFYPSRDFGATPRD
                     AGIAAEDVFIEVVPGQRIHAWYVPPPEPA"
BASE COUNT         8123 a        15377 c        15593 g         7419 t
ORIGIN      
        1 tgtaccggct ggcgctgtcg gtggacgggc ggctgtggca cgaatcggtg ttcgactccg
       61 cctatttcga aaccaacacc gccgtctcgc tcgagtatga cctgctcgaa gccgtcgccc
      121 agcgcaagca cgtgcggcgg ctgtaccggg aaccggggaa cgactacgcc gggtgccgtc
      181 cccatggccc ccatgggggg cttatcggct gcgccaccgg gctggcgccc ggcccgcacg
      241 acctcgcgat cacagcggag gatgctttcg ggaacaggtc ggaactccgg ttcagggtcg
      301 tgctcgggcc gggggagttc ctctacgggc tcgattcgac cgtgcgggtg gcagcggaca
      361 cgaccgattt ctatttcacg gccagcgaag cactggccac ggtggcggtc gattcgatcg
      421 tgctctatgt cggacggggc gaccgctggg gctggacgcc cgacgggacg atcgagcggc
      481 tcgacggcgg gcggctgaaa gtgcgcgccg gcggctacaa aacccgcgtc acgccgttgc
      541 gcctctacct cttcactgaa gaggggggcg tgatcgccga cggcgtgttc aacggactgc
      601 gcgatgacga caccggggga aagttcgaaa cctcccacga gctgaccgag gacggcctgc
      661 tcatcaagat cacctcgctg gaatcctcgg cctacctctc gcgcgtcgat ctcttctacc
      721 gggggaaact gctggggacg gagtacgggc ggatgctcac gcccaagcgg tatgtgtgcc
      781 tggtgcggcc gcaggccaag tacgcgcgga tcgaccggta ccaggcggtc ctctcgcacg
      841 acccggcgcg ggcggggcgg tggtccgact ccctgaatat cgtcgcggcg gggatcgatt
      901 cgacggtccg ggcggccttc gaccaaaacg cctggctcga tctgcccaaa gagtccttct
      961 tcgcccctgt gttcctcgaa ctcaagagaa acgccgtcgt cgaccggtct cccctggccc
     1021 tcaattccga ccactacctc gtgctgccgg agaccttccc gacccgcacg cgcttcgcca
     1081 tcacgtacca cataccgtac gacgtggagc ccaaccggta cagcggtctc tgctggctgg
     1141 acaagaacac caatcggtgg gtgtggctgg agaattcatt cgacgacgac cgcgacgtgc
     1201 tgacggcgac ggcgggggga ggcgggtcgt tcggggcggt gtttgattac gaaaccccgc
     1261 ggatctcgaa tctgtcgctg cgcgaaggcg gggtgtacca gaaccgccgg ccggccgtca
     1321 atttcgtcct cgaggacacc ctgtcgggca ttgccgacga tcggaacatt gtcatcgagc
     1381 tcgacggcga gtggctgatc ccggaatacg agcccgagac cgggcgcgtg cacagccggc
     1441 cgctggcgcc gctggcgccg ggcgaacact acctctcgat tgaggccacc gaccgcgtgg
     1501 gcaacaagac cggcaagtac cttcgctttc gcgtcgcgaa agcggggggc ggcgaccggt
     1561 gacggctgag gcgaaaattg tttattccca tacgcgatga tactccgact atccgcagac
     1621 cgtacgtgac ggtgggcctg atcgccgcca acacgctcgt gtttctgtac tccatcatgg
     1681 gcagcccccg ggctttccag ctcttcattg tccagtacgg tttcatcccc tacgagctcg
     1741 tgcatttcac ggaactcacg ccgcaggtcg ccgcgccggt gtggctcacc atgttctcgt
     1801 cgatgttcat gcacggaggg tggatgcacc tcctgggcaa catgctgttc ctgtggatct
     1861 acggaaacaa catcgaggac tacttcggcc ctgtccgatt catcctgttc tacctgttgt
     1921 cggggctgtc ggcgattttt ctgttcacgc tcttcggccc caattcgcag acgcccctgg
     1981 tgggcgcctc gggcgcgatc tcgggcgtga tgggagccta cctggtgctc cacccgcgtg
     2041 cccgcgtcac cgtgctcatc atcttcttct tcatccagtt cgtcgtgctc ccggccaaaa
     2101 tggtgatcgg gctgtggttt gtctaccagt tgctgctgtc ggtggtcgac gccatccagc
     2161 cgagcggagc ctcgggcggc gtggcctacc tcgcgcacgt gggcgggttc gtcttcgggt
     2221 acctgctgct gcggggattg ctcaggttcc gggggccgcg gggaagggcg gacgacggac
     2281 agcggattta ccgcgtgcgc tgggattgag ccaaccacgg cccggccgac cggcgtacca
     2341 tcaccgggaa atgggagatt gagtcaaatg ccctgcaagg atatcacgga tacggtcaag
     2401 ctcacggtcg cgcccgacga tcgggtggtc gattaccggc tgttcaaacg cacgtgcggg
     2461 ggcacggtcg gtgaagacaa gctgatcggg ccctggctgt tccggcgcac ggcggcccag
     2521 gtggccgcta cccgcaacga ggattttctc gccaagctgc gggccgggag cgacatcacc
     2581 gagtacctcg ccgtgaagca cttcgccgcg gtgcgggcgg ccctccaggc cgcactcggg
     2641 ctggcggcgg ggcgggtggg ggactactgc actatcgaag ccgtcgaata cgggccggag
     2701 ggaatggcga ttaccgccca cctggcggtc aaggggctga ccgacgagat cgccgcctgt
     2761 cggaattgct gcgggcgcaa tcggtcggga gaagaaacct tcggcgtgta gggggatttc
     2821 cccaacctga gaaccccgcg gccccggtcg ctggaccggc gcccggctat acgacgcagc
     2881 cgcactggag aggcgggccg gcctggaaga acgccgctat catcgcggtc agatcggcga
     2941 catccggacc ccggcaatca ccgttgacat cggcggcgtt gagcgacccc ggcggcggac
     3001 cgttgcggaa caggtaggcg acgaaatacg tgaggtcggg aatcgacacc gcaccgtcgc
     3061 tggtgagatc gccgggaacc acaggaagaa cgtcgacccg gtcgcgaaag tagttgaagc
     3121 cgggatagat cgtccggttg tcgagactcg cgtaagtcag gcggcgggcg acgcttccgg
     3181 ccagcgccgt gtcccagcgg aaggagaaac tccccgcgca cgagttgttg ccggtgaggt
     3241 agcggatgtt gcacagggga gtctggccgg ccgggatcgt aagcccggcc gtattggcca
     3301 gcatgatgaa cagaaacccc gaatccggct tgatctcgag cagggcgata ttccagttct
     3361 cggtcaccag gccctcggtg gagagctcga ccacctccgg cccggcgggg attttaatcg
     3421 gaatcgtcgc gccgcgaatc ggggcgacca gtttcgccac caccggctgg acgccgaatc
     3481 cgccgtacac ggtccgcgag ggcagaagca aagaatcgcc gggcgccgag cgcgccccgg
     3541 ccgcggacaa gaggaaaatg cagatcacca ccgcgacggc gctggagaga cggcgcatgg
     3601 acaacacgcg gggcctcccg ccaacaggag ctctgggcca atcagtcatt tctcattcaa
     3661 agtacccgcc gtgggaccga ttgtcaaaag gtattcgcgg cgcacgcata ggccgggcgc
     3721 gacgccggcc gatgagatcg tcggccttga catttcggcg tttgccgtta tattgcggtt
     3781 gcacggacga tcgcctcgaa attggttagc cggtcgttct taattggact agttctgttc
     3841 gggctctaac gctatgacgg tatcggggag actggcgtcg cggtggcata tgatttcgct
     3901 gcccgccttg accgccgcgc gcttgcagtg ttcgggagtg cgaacgtaca atgtgccatt
     3961 gatcgcgatc acctgccgcc ggtccccctt ggctgacgca ttgcggtttc agagattcat
     4021 tccatcttgc catgcgtctt tcaacgattc ttgcgacttc tcttggcctg cagcattgtg
     4081 gcaacatggc aagtaatgac ttcatggata atctgcggcg gatcatggcc gacaggcgat
     4141 tgacgattaa cgaactcgct tgcaggtccg gcgtgcatca cgtcatgatc cggggatatc
     4201 tgcaggaatc cgaccggggg aagttgccgt cgatagagac ccttgtggcg ctcgccaggg
     4261 cgctccattg cgaccttgag gcgctgaccg gccacgagga acttcggggc gtggtggagg
     4321 tgcccaagtc atccccggac gccgaagcgc tgtatgatta ttttacgtcg ttaccggagg
     4381 gcgacacgat gcgggagatt ctgcagcgga agatatatca aatgctcgaa gacaacatga
     4441 agaaggagaa cgggacatga agggcttgac aatcgttgta tttttgctcg ttgtggcggg
     4501 ggccgcctat gggcagcgtc tcgagaaccc ggatcagcgt ccgagtctga tcatggggat
     4561 gggatacagc tggatgaatg atcgatgtga aacgactgtg gcgccatatg tcatcgatgc
     4621 tgtccgcctc ggccgcctga tacctacgtt cggagcatcg gttccggtca gcaaacatgt
     4681 gacaatgctt ggatcgatgg cgtggggaat gcgacggact gtcgggccgg agttatctgt
     4741 ccccgtcagc gaatattctt cggtgtcggt tcagcgatac ttgaccacgt tcaatatcga
     4801 ggtggcggtg aaagtttacc tggacagaga ttagtgggga ggacgacaaa tgaaaaggct
     4861 cgtgtctcta ctcctgctgt tgccggttat ggccgcagcg cagcagaaca accctgatcg
     4921 agctgaacac attgagctcg gtctcggatt cgagcaaacc ggcaccgatc ttctgatcaa
     4981 tagcgtcgac tacagaaagt tcgacgtggc gggtctgaag ctctatgcca actatcttga
     5041 cccggtgagc gaccggctga cattcatcac gagcgtggaa tggctgccgg atagtcaggg
     5101 gacaggatat gagctcttcg gttccactat cgaggaatcg acccggcgtt tcagcatcat
     5161 gtttggcctc aggatgtatt tctcgggcga gagcgaccgg ccctgactgg gagacgtgat
     5221 tggcaaattc ggttcagacc aattaaccgg ggctgtcagt atatgtcaat aaacttacgc
     5281 gggaggggcg gaaaaacggc tcggccgagc ttgcgatgtc gggcggcaac ccgcggcgcc
     5341 ggcggcgaaa ccgcccggag gccgccccgg acaggagcgg tcaaatccgc ttgacttggc
     5401 ctgttcagcc cgtatattcg cgcatgtgaa attctgaaca tggggctcag tggtcagcgg
     5461 gaaatcgccg cgggagcccc tttcgcatca aaggagtcag ttcgccatga ttactcggca
     5521 agaagccctc gactaccaca gccggccgcg caccggcaag atcgaggtca ccccgaccaa
     5581 gccgtgtcgc acccagctcg acctctccct ggcctatacc ccgggggtgg cgcagccctg
     5641 tttggaaatc cacgagaatc cggaggacgt ctacaaatac acggccaagg ggaatctcgt
     5701 ggcggtggtc agcaacggca cggcggtgct cggccttggc aacatcggcg ccgccgccgg
     5761 caagccggtg atggagggca aaggggtcct cttcaaacgg tttgccgacg tcgacgtatt
     5821 cgacatcgag ctgaatactg aagacccgga cgaagtaatc aagtgcgtcc agctactcga
     5881 gccgactttc ggggggatca acctcgaaga catcaagggg ccggagtgtt tctatattga
     5941 ggagacgctc aagcggacga tgaagatccc ggtgttccac gacgatcagc acgggaccgc
     6001 catcatctcg gcggccgccc tgcttaacgc gctggaactg gtcaagaaag acatcgacaa
     6061 gatcaaggtc gtctattcgg gcgccggcgc ggccgggatt gcctgcgcca agctctactg
     6121 ctcgctcggc gtcaagcatg agaacctgct catggtcgac tccaagggcg tcctctatga
     6181 ggggcgcggc gacaagtgga acaagtacaa aggggagttc gtgcgcaaga ccgatgcgcg
     6241 gaccctggcc gacgcgatgg tggacgccga cgtgtttgtc ggcgtgtcga cgaaagacct
     6301 ggtgacgaaa gagatggtga agtcgatggc gaaggacccg atcatcttcg ccatggccaa
     6361 cccggacccg gaaatcacct accccgaagc gatggatgcg cgcaaggacg tgatcatggc
     6421 gacggggcgc tcggactatc cgaaccaggt caacaacgtg ctgggtttcc ccttcatttt
     6481 ccgcggggcg ctcgatgtgg cggcgacggc gatcaacgag gagatgaaga ttgcggccac
     6541 ccacgccctg gcgacgctgg cccggcagaa cgtgccggag tcggtggcca aagcctacgg
     6601 cgtcgaccac ttctcgttcg ggcgcgagta cctcatcccc aagccgtttg attcgcgcat
     6661 cctgatctgg gaatcgtcgg cggtggccaa ggccgccatg gagacgggcg tggcccgcaa
     6721 cccgatggat atcgaggagt acaagaagct gctttcggag cggatcgggc aggggcggaa
     6781 ggtcatgacg ttcatcagcg agaaggcgcg ccgcagcccg aagaagatcg tgttcccgga
     6841 gggccactcg tcacgcatcc ttcgagcggc gcggatcgta aaagacgaag gcatcgccct
     6901 ccccatcgtg ctgggagatc cggcggacat caagaagatc gcggccgagc acgaggtcga
     6961 cctgctgggg atcgagatca tcgatccggc cacctcgccg cgccgtccgg cctacgccca
     7021 ggcctactgg aaacgccgcg cgcgccgcgg gatcaccgag gacaaggccc tctggctgat
     7081 gcgcgagcgg acgtatttcg ggatgatgat gctggcgacg ggcgacgccg acgcggtgct
     7141 ctccggcgtg ctgtcggatt acccatcgac cattcgcccg gccctggaaa ttatcgacct
     7201 cgctcccggc gccacccggg tctcggggct gtttgcgatg ctccagaagg accgggtgta
     7261 catgttcgcc gacaccacgg tgaacatcaa cccctcgccg gaggagctgg ccgagatcgc
     7321 gctgctgtcg gccgacgtgg cgcgccggtt cgggatcgaa ccgcggatcg ccatgctctc
     7381 gttctcgaat ttcggctccg cccggtatcc cgagtcggag aaagtggcca gggccacgac
     7441 tctcttgaaa aagatggcgc cggatttggt cgtcgagggc gagctgcagg cggacgcggc
     7501 ggtcatgccg gagatgctgc gcaaactgtt ccccttctcc aagctccagg aaccggccaa
     7561 cgtcttcatc ttccccgaac tcaactcggc gaatatcgcc tacaagctgc tccagcgggt
     7621 cggcgggctc gacgcgatcg ggccgatcct catggggctg tcgaaaccgg tccacattct
     7681 gcaccggacg ctcgatgtca acgagatcgt cgacatggcg gcgatcgcgg tggtggacgc
     7741 gcaggcgccg acgggcggga gcgcccgggc gaaatagggg gcgcgacggc ggacaatcac
     7801 ggccggggcc cgcgccccgg ccgttttcat atgctccccg gacgccccgc cgggcacttt
     7861 tttgttgccc catagcggga tagcctgtaa actattggac accgtcgttc gataacatgt
     7921 tcagggatga ccgcaccgac gcgtttggcg aaagcgtaaa aaggatttga cgtgggattc
     7981 ttcgacctgc tcagcaacga catcggtatc gacctcggta ccgccaacac gctggtctac
     8041 gtaaagaacc agggaatcgt cctgaacgaa ccgtcggtcg tcgccatcga acgcaccacc
     8101 ggcaaagtgt tcgccatcgg cgccgcggcc aaagaaatga ccggacgcac gcccggcacc
     8161 atcgaggcta tccgccccct ccgcgacggc gtcatcgcca atttccagat ttccgagaaa
     8221 ctcatttccg atttcatccg ccgcgtggtc aagcacaagt acctgatgaa gccgcgcgtc
     8281 gtcatctgcg tgccctcggg aatcaccgag gtagaaaagc gggcggtgcg ggattcggcc
     8341 gagaacgccg gcgcccgcga ggtctacctg ctgcaggagc cgatggcggc cgcgatcggc
     8401 gtcggcctcc ccgtcgaaca acccaccggc tccatggtca ttgacatcgg tggcgggacc
     8461 tcggaaatcg ccgtcatcgc cctcagcgga attgtcaaca acacctcgat ccgcgtcgcc
     8521 ggcgacgaaa tgaacgaggc gatcacccag tacctcaaga agaactacaa cctggcgatc
     8581 ggcgaactca ccgccgagga tatcaagatc aagatcgggt cggccttcgc cctcgacaag
     8641 gagatctcga tggagatcaa ggggcgcgac ctcgtggccg gcgtccccaa gaacctcaac
     8701 ctgtcctcgg tgcaggtgcg ggaagccctc tccgagacga tcgacatcat cgtcgaggcg
     8761 gtgcggcagg cgctcgaaca gacgccgccg gagctggcct cggacatcct cgagcgcggg
     8821 atcatcctga ccggcggcgg ggcgctcctg cgcgggttgg acaagcgcct gcgccaggag
     8881 accaatctcc cggtcaacgt ggcggatgat ccgctcacct gcgtggtgcg gggaaccggc
     8941 aaggtgctgg agaacttcac cgccttccag aaagtgctgg agcggccgcg gaaagactga
     9001 cccgcgccgc cctcggacct ggccgggccg gactgccgtc cggccttttt gcatacactc
     9061 ctccgcggcc attaatcttt tggattgccg ggccggtctt atcggcggaa attaaatgca
     9121 tggctgggaa cgaccgtgag acaatcgacc gcctggtcag gcaggcgcaa aacggcgacc
     9181 gagcggcgtt ctcgcaactg gtacggcgga ctatgagaga cattaccgcg ctgacctacc
     9241 ggatgaccgg cgaccgggac gccgcccagg acctggcgca ggaaaccttc gtctccgcct
     9301 ggcagcacct ctccgggttc cgccgagagg cccgcttcga gaactggctg taccggattg
     9361 cggccaacaa ggcgctgaac tacctgggcc gcgggggggc cggccgcacg gtgtcgctcg
     9421 aggacgccgg cggggcgggg gccgagcgcg cctccgatga acccgcgccc gaccgggcgc
     9481 tggagcaggc ccgcctccgc gacgacatca gcgccttcat gcggctcctc cctccccgcc
     9541 agcgcctggt gttcgagctg cgcttctacc gccagctcgg gttcgccgag attgccgaga
     9601 tgaccggaac cgcggtcgga acggtgaaga ccaactaccg cgaggcggtg ggcaaactgc
     9661 ggacgtatgc ggcggagagg gggtggcggc catgacctgc gccgaagtgc gggacatgct
     9721 ccacgaatcg ctgggggccg atgagctttc gggcgaggcg gcggcgcacc tcgccgggtg
     9781 tccggcctgc cgcgctctgc acgaggaatt gcgggcgacg gccgaggggg cgcggacagc
     9841 gggcgagttc gaactcaccg acctcgagag cgcccgcctt ctggcgcgga tcgacggcga
     9901 gatcgaacgg ctcgatgagg cccagcgggg ccgaaccccc gcctggctgc cttggctggc
     9961 gccggccgcg gccgcggccg cgctgatcct cgcgatcgcg ctgaccggcg accgcttcga
    10021 cccgcgtgtg gcggtgacct ccgattcccc ggcgaccgcc ggcgaggcga ccgcggccgc
    10081 cgaagattcg ctctatgacg atctcgacga gccgaccgtg caggcgctcc tggaggatgt
    10141 gagcgcccgc cccgccggcg aaccgagcaa cgccctcctg gatgacctta ccgacgacga
    10201 atacgagtac ctcgccaaaa acttcgacat aggagacatt ctgtgagacg ctacgcctcc
    10261 gtggtgctgg cgggaggtct ggtcctgtgc ggcggagcgg cgcaggcgca gggtccggaa
    10321 accgggccgt gccccggcaa aaaccgtccc ggaccggaat gcgcgatcgt gcacgcggcg
    10381 ctctacgccc aggcggaacc gccggcggcg cctgaggcgc ccgaggcttc cggaccgcgc
    10441 cggcgccacc tcgaacagtt ccgcatgctc aaactgctcg aacttctcga tctggacgac
    10501 aagcaggaaa tggagttcct cgtcgagttc aaggccctcc gggagcggca gctcgtgctg
    10561 cagcaggaac gccggcggct ggtggaaaac ctggccaccg gcctgcgcga ggatacgctg
    10621 aacgactcgg cgatcgccga gctggtcgcg cgcgtgctca ccctgcgccg cgaatacgcc
    10681 ctcgccagcg agcagtttct cgaccgcgtg gcgggcatcc tcaccaaggc gcagatcggc
    10741 aaactggtgg tgttcgaaga acgttttgaa ttcgaactgc tcgaccaggt gcgttcgttc
    10801 cgggagcggc agggaacgcc cggcagcggg tggcgccggc agccggggga ttcggactga
    10861 ctccggacag cggcaatcgg ataattcggt gaaagaatcg gaaaggagac tgacgatgag
    10921 aaaggccatg gtggttttac tggcggtggt ggcggcggcc gcgcttgctt gggcgcagcc
    10981 cgccccgggc ccgaactgcc cgcaagcacc gcagaccccg caagccccga tggcgacggg
    11041 tatgggcgcc ggttgcggga tgcatggcgg ccagggcatg atggggtgcg ggaaacatgg
    11101 cggccaaggc atgatggagt gcgggatgag gggccacggc cggatgggcc gcgacatggg
    11161 agactgcggc ggcggcgccg gcctcgcgca gttccggaaa gaactggatc tctccaatga
    11221 tcaggtcgaa cggctggagc agctcaagct gaaccaccgc atgaacatga tcggcgcccg
    11281 ggcggacgtg gaacgcgctg aactccgact taaggagctg ttgcgcaaag atgacgcctc
    11341 gacgcaggaa gtcggccagg cgatcgatca ggtggcccag cttcgcgccg gcattcagaa
    11401 gcagtgctac tcccagcgcc aggaatgcct ctcggtgctc accgacgcac agaaggaaaa
    11461 actccgccag ttgcgggaag aacgccggga cgagcggcgg gagtttcgcc agggccaggg
    11521 ccgggaccgc atcgagttcc gcgaccgccg ttttgggacc tctcggtaaa ccggcttttg
    11581 cgttagcagt gtgggcggga gaccggtggt aaaatcccac cggtctccct ttatgggcac
    11641 ataactccgt ggcgcaggga agacttttgt gtataataag ttgcaccaat gatctgccat
    11701 tttggccgat tggtgacaga tgagacttga cagccgtttt tgagaaggtt acgttctggt
    11761 actatggcac aaacacgcaa ggaaatactt gaatttatcc gggccaagtc ggcccacccg
    11821 atgaggatga aagaattggc gcgagcgctc ggcattcccc ccgaggaata ccgcgagttc
    11881 cgcggccagg tgaaggcgct ggtcgacacg ggggagctgg tgaagcttcg ccggggccgc
    11941 atcggggtgg ccaccgaaat ggacgtgatc gtcggcccga tctcggttaa ccgctcgggc
    12001 gtcggctttg tcgcgcgcga gggggagccg actgacatca tgatcccggc gacgcagttg
    12061 agcacggcgc tcgacggcga ccgggtgatg gtccgcctca ccggccacct cggcgaccgt
    12121 ccgaccggca gcgtggtgcg ggtggtcgag cgcacccggc gcaacattgt cgggaccttc
    12181 caaaaaacgc cgcacttctt ctgcgtgcgg cccgacaatc cccgcattca ccgcgatatc
    12241 tacatcccct cctcgcacac cgccggggcc agagagggag agaaagtcgt gtgccgcctg
    12301 accgtgtggg atgaccccta tgtcaatccc gagggcgaga tcagcgagcg gatcggtttt
    12361 cccggccagc ccggcgtgga catgctcacc gtgatcaaga gtttcgacct ccccgaggaa
    12421 ttccccgagg aggtgcgcaa cgagggcgag aaggcggccg cccgtctgag cgaagagccc
    12481 gaggagggcc ggctggacct caccggggaa tgcatctata cgatcgaccc ggccgacgcc
    12541 aaggaccatg acgacgcggt gaatgtcacg cggactcccc gcggctatcg gctaggcgtg
    12601 cacatcgccg atgtctccca cttcgtgcag ccgggaacgg cgctggacgc cgaagggttc
    12661 aagcggggga actccgtcta cctgcccggg atggtgatcc cgatgctgcc ggagagcctc
    12721 tcgaacgacg tctgttcgct caagccgaac cggaagcgcc tgaccttctc ggtgttcatg
    12781 gatttcgacc gggcggggaa gatgctgtcg tgggagatcc ggccgtcggc gatcaagtcg
    12841 cgggccaagc tgagctacga ggaggtgcag gagtttttcg acaaagggct ccccgagggc
    12901 gcagggcggc ggaagctcga gcgggtggct gagaatctca cgacggcccg cgagctcgcc
    12961 cgcctgctca gcgctcgccg gttcgccgag ggatccctcg atttcgacct gcccgaagcc
    13021 aagatcaccc tcaacgaaaa gggggaggtg atcgagctgg gcaaccgcgt gcgcctggaa
    13081 tcgcaccggc tgatcgagga gttcatgctg tcggccaaca aggcggtcgc gctcgagatg
    13141 ttccgcagtg cccaggcctt cctctaccgc gtccacgacc gccccgacat ggaaaaactc
    13201 gaggccttct cggccatggt ggcccgcctc ggataccgct tccccgtctc ccccacgacc
    13261 aaaccgggag acctcgcccg cttcctggaa acggtgaagg acaagccgga ggccgaattc
    13321 atcaacgagc tgatgctccg ctcgctgaag aaggcggtct accagcgcaa caatgtcggc
    13381 cacttcgggc tggcgttcac ccactacacc cactttacct cgcccatccg ccgctacccc
    13441 gacctgctcg tccaccggct gctgcggatg cggcggcccg acgggaagta ctcgccggcg
    13501 gcggcccgac ggattcctgc agtcatcgac cacgccggaa aacactgctc ggagaccgag
    13561 cgcatcgccg aggccgcgga gcgggaggcg gtgaaggtga agcaggtcca gtacatggcg
    13621 cggcacgtcg gcgacttctt cgggggcatc atctccggag tcaccggatt cggcttcttc
    13681 gtccgcctgg acaacctcgg cgccgagggg ctggtgcggg tatcgacgat tgacgacgac
    13741 tactaccggt tcgacgagca gcgctacgcc ctcatcgggc ggcggacggg gcggatgttc
    13801 cgtctgggcg accgggtgga ggtgggggtg ctctcggtgg acaaggtgaa taacgaaatc
    13861 gcgctctttg ccccggggct gcccgctcaa gcgaaagccg ggagcgggcc gaaggggaag
    13921 cgcaagaaga aagggtgggc ggaggcgcag ccgcccaagg ccaagacccc cgcgcggacc
    13981 aggaccgccc cgccggggta cgggcgcacc cgcaagaagc gcccgcgctg agacggggaa
    14041 tcgaccgcca tggtaaaccg cacgatcata ctcctcgcgc ccgaaaccgc cgtcgcctcc
    14101 gggatcgcct gggagcgcct cggcgaaacg gtcaccgagc tgtcggagct ctaccgcctc
    14161 gtgcgcgagc ggcgcgtgga tctggtcctg gtcgatgaga atcgggccga cctcaaacgg
    14221 ccggtggccc gcagaatccg ccgccagaac ggcctgaccg aaatctggct gctcacccac
    14281 gaccgggagc cggccccggc cgaggtgcag tttatcgacg ggcgccttcc gctgtcgctc
    14341 gggccggacg ggctcgcgca aaaaatcgcc cacatctacc acggcaagca cctgctcgaa
    14401 cggtacgata tggtggggcg cgcgccgcag atgaagatcg ttgcggaaac gatcgagcgg
    14461 atcgcgccga ccgacgtctc ggtcctcgtc gtcgggcagt ccggcaccgg gaaggaactc
    14521 gtggcccggg ccctgcacac gcagtcggcg cgcaaggacc ggccgtacgt ggccatcaac
    14581 tgcggcgcgc tcgccgaggg cgtgctcgaa tcggagctgt tcgggcacga gcgcggagcg
    14641 ttcaccgggt cggtcgccag acgcgaggga ctgttcgcgc gggccgaagg ggggacgatc
    14701 ttcctcgacg aaatcggcga aaccaagccc gacacccagg tcaaactgct gcgcgtgctc
    14761 gaggacggca cctactaccc ggtgggatcg tcgacggcgc ggcatgccga cgtgcgcgtg
    14821 atcgcggcca ccaaccgcga cctcactgag gccatcagcg aaaggcgctt ccgcgaggac
    14881 ctgtacttcc gcatcggcgt tgtgaaaatc gtcctgccgc cgcttttgga ccgcaaacag
    14941 gacatcgagg cgctcctgca gcatttctgg cgcggaaaag agcttgacta caccgacccg
    15001 gcgctcgatc ttctgttgaa atacgactgg tcgggcaacg ttcggcaact gaagaatttc
    15061 gccgatcgca tgcgggcgct gaaaccgcgc gggacggtgg aggtcgagga tgtcgaacgg
    15121 ttcatcgacg agcaacacgt cacggcaaaa cacctgccgg tcacgaccgg caagacagtc
    15181 gaggaggcgg gacaggagtt aatctaccgg gcgattctcc agttgggcgc cgaaattcga
    15241 ctcctgcgcg acctcatcac cgcccacctg ccgagcgaac cgcccgagga gacggccgag
    15301 gccgcgccgg cggccgacgg cgcgaccatg gaagaggtgg aagaacgcat gatccggcgg
    15361 accctgcgcg aaaccggcgg caaccgcaag gaagccgcgc ggcggctggg gatcggcgag
    15421 cgcacgctct accggaaact caagaagtac gggctgagct gacaccatgc gagcggtcac
    15481 gctgaccgcc gcaatcacca tcgcactcgc ggccgcggga gggaccgccc aggaactctc
    15541 gaccgacgtc tatccctccg aggacgagtt gctcgaggcg ctgcggcggg gcgacatcga
    15601 tcaggagcgg tacgacattc tccgcgaaat cctgcgcgag ggaatcgacc agccgtcccg
    15661 ctacctgttc aacgaaatcc ccaatctggc cggggaggaa cgcgccgaca cggcgctccc
    15721 gcgcctggcg gcggagcagg ccgaagcctt caccgccaag cggccgccgg cgccgcggct
    15781 ggcggtcagg catcggtacg cgcaggagat cgagcgcagc ggaagaggaa gctaccggac
    15841 cactgtctcc gggcggctcg gcccgcatct cgacggcgcg gtgaggctgg agcggacggt
    15901 ttccgggcgc gaacgggtgg tgggaaggat gatccgctgg cggcccgagc ggggcgtgat
    15961 ttgcgaggcc gcggtcggat ccctcgcccg ccggctgggg ctcggaacgg tggccggcta
    16021 ccgtggaaag atcctcggga gttcgccgcg gctcgacggc gagtcggtcc ggtaccccga
    16081 ctacggcggg cagaacggcg gatatgtccg cgcgctcgcg gggcggtggc aggccgaagc
    16141 gctcgtctcg cacacccgcg acggggagtt caccctgctg tcggccgcgg tggcggtcgc
    16201 ccgccggtgc ggcaaagtgc gggccgcatc gattgcaggg atcaaccgcc tgcgcagccg
    16261 cgtgcgcggc gagccgcgga gcgatgtcaa gtacgcaggg ttgctcgaat atgcctacgg
    16321 caatggccgg atcgccgcgg aagcggccgg gcaggcgggc gtgcgcagcg ccttcggcgc
    16381 cgtcgttttc gaaggacggc accgcctgac gggggccgag atccgctact gcgggtggca
    16441 ctacgatcgc gattatgtcg atctggcggg cggcagcgcg ggggggacga tcgccgcgcg
    16501 agacagcatt cccggtccgg cgttcgcgtt ctcctcgcgg cgcaccggca acaccggcgc
    16561 cacggtgcgc accgaggccg ccctgacggc gcgggtggcg ctcgagaccg gcgtggccgg
    16621 atccatccgc agcagcgata cgctgcgcgg cgaggtcctg gcggggctta tcgccgccgc
    16681 gagccgcgcg ctgcaggtcc gggccgatca cctggagcgc ctcacccgca cgagggagag
    16741 cctgtacgac cgcgacgccg tcgtgcgccg cacgcgcgcc gaggtgcggt acgatcgcgc
    16801 ggccctggcc gtgcgcacct atgtcgggta caccgtccgc agcggcgccg ccgactcctg
    16861 gaactggttc gcgcgtctcc gctaccgctc tgcgcgggtc ggggatctcg aaatctgggc
    16921 gaacctgggg acgatctcgc ccgcgcgcag acaaattgac tacgcctacg ggtatgtcca
    16981 gttgcggcaa atgatcgggg aggcggtggc gatcgcggtg aaaatcgcgc agcgctatag
    17041 ccggggagcg gacgaccctc accgcacgac ggtggcgctc gaggccgaag cagtcctgta
    17101 gcgacgcgag gtgacgcatg gatctggcat gaaatggggg atggttttga cgccggcagc
    17161 gaaaagaaac aattgacgac cataacggtc cggacctgca cgaacacccg gatacagggg
    17221 aggaggtacg atgcggaagg tcaggaattc ggccgcggcg cggtctcagg gtgccggcga
    17281 gataggcgcg ccggccgccg cggcctcggc ggcgccagcc caggcggagg tcctcgcgct
    17341 ggcgaaggcc atgctggggg cgttcgaagg gacgctcggt ttcatcctgg cggtcggtgc
    17401 cctcctggcc ggtgcggcgg cgccggtgca ggcgatccgc cttaatgaag tgctcgccaa
    17461 tccgaccgca ggttatggat cggagtggat cgaactggag aacgaaaccg ataccgccgt
    17521 cagtctcgcc ggatggcgga tcggagacgc gctgcggaca gcggtgatta tcgacaccgg
    17581 agcgggcccg gctgtttcag acgcggctgt cccgccccgg ggatatgccg tcctcgtgca
    17641 ggattccgcc gcattcttcg cctccgccgg ccggccggaa gcgccggtga tcgagccgcc
    17701 ctcgtggccc gtgctcaaca acaccggcga cagcatccgc ctgatcgccc cggggggcgc
    17761 gctccacgat cacttcgtgt atcgggccgt gtttccggac aaccggacct ggtgccgcca
    17821 cccgatcgac ccctcgcggt gggaccggtc ggccgatccg ggaggcacgc ccggccgaga
    17881 gaacgtcctg cccgactctg atgccgccgg cgcggcggcg ctgaccgtga cgcccagggt
    17941 gttctcgccg gacggagacg gcgtcgacga ctccaccgtg attgggctgg aggcggacga
    18001 ggagctcgac cgccgggtgg agatttacga tcgcgaggga cgacgggtgt gggagatggc
    18061 gatcccggcg gggcagcggc aggcgcagtg cgtgtggtgg gggcggtcgc gagcgggcga
    18121 gcgtctcccg atcgggatct acatcattta cctcgaagcg atcggcgggg agtcggcgcg
    18181 ccggaccgtg gtgatcgcgc gatgagacgg ccggggcggg cggcgatcgg cgcgggtctg
    18241 cttttggcct gcgccgccgg cgccgcggcg ttcgaattgc cgacgccgcg cggcgaaggc
    18301 ttcggccgga ccatgctcct ggccgacgcc tcggccgccg cgctgaccaa ggcgccgacc
    18361 gccgggatcc gcacggggga gtggcggttc gaactcggcg gcgtgcggcg ctttgaactg
    18421 agcgacctcg accaggtgtt cgccgccgcg gccggacgac tgggccgggc ggtgctggcg
    18481 gcgggcgtgt cccagttcgg caatacggag ttgtatgccg agacgacggt ccgtctggcg
    18541 gccggctggc gggtgggcgc attcggcgcc gggggcagcc tcgccgcgaa gatcgtcgaa
    18601 ttcggcggcg ggtacggccg cctccacgcc gcggcggtcg gcctctcggc aacctatgcc
    18661 gggaagcgcg tgcaggcggc ggccgcggcc gagaatctta actgcccgcg cctggacgcc
    18721 gccgccgcgc ccgaccatcg ccggctggat gtgtacgtgg aaatctaggg gaacggcgcc
    18781 taccagacga ccgggcgggc gagttttgag ccgacgcagt ccccccggtt cggcatcggc
    18841 cagcggttcg cggtggcgcc gccggccgca atattcgtgg gcctggcctc tgagccgttg
    18901 accttcggcg ggggaatcga tatcatgcat cgcggcgccc gcctcacgta tgccgccggg
    18961 taccacccgg tgcttggcct gacgcacgcg ctggcgctcg ccctggaaac cggccgatgc
    19021 aggtgaacgg atggaaccga tactgacccg ggaggagatc gagcagcggg agcgggcaac
    19081 cctggcgccg tatgcggcgc tcgcggccga atcgcgggga cggcgctttc cctcgcgcga
    19141 ccaccccctg cggacggttt tccagcgcga ccgtgatcgg gttgtccacg ccgccgcctt
    19201 ccgccgcctc gaatacaaga cgcaggtgtt catcccccac gagcgggacc atttccgcac
    19261 ccggcttacg cacacgatcg aggtggcgca gatcgcgcgg acgctcgccc gcagcctccg
    19321 cctcaacgag gatctggccg aagccatcgc cctggcccac gatctcggcc acaccccctt
    19381 cggacattcc ggggaggacg tgctgaagga tctgctcgcc gggttcggcg ggttcaacca
    19441 caatcgccag accctgcgga tcgtcgacct gctcgagcag cgctatcccg agcacccggg
    19501 gctcaacctc acctatgaag tacgcgaggg gattgtcaaa cacgagacgg tgtgggacca
    19561 caaggtggcg gagttcgacg accacgcgca cccgacgctc gaggcggcgc tcgtggacgt
    19621 cgccgatgaa ctggcgtaca acgcccacga catcgacgac ggtctgtcgg cggggatgct
    19681 cgccttcgac gacctgtgcc gccagccggt ctgggaagtg ttcgggctgg acgggcgcgg
    19741 ggcgttcgag cggctccatg aggaccagaa acgctacgcg ctcgtgcggc ggctgatcga
    19801 cctcacggcc accgatgtga tcgcccacac cgccgcgcag atcgcgcgct tccggatcgc
    19861 gagtctcgcc gacgtgcgca gcgcgccgga gaagctgacg ggctactccc cggatctgcg
    19921 cccggcggtc accgcgctca agcagttcct cttctccgac atgtaccaga gaccgcgcct
    19981 cctggcgctg gcgaaacggg cgcgcaccat tctcgaggga atcttcgacc gcctcatggc
    20041 cgaacccgaa cgtttgccgg cgcggttccg ggagatgctc cagaccgaag aaacggaaat
    20101 tgtcgtcacc gactacatgg ccgggatgac ggaccgctac gccgagaaag tgtaccggga
    20161 gctcacgggg aaagaaccgc cggatctccc cgaaatgctc tagttgccgc cccgctcccg
    20221 cagccgatta ttcattggta aaatcggcct tccggtgtat attacgggca atcgaagttt
    20281 ccgcctggtt cggaggacaa catggcttct caccgcatgg ggacggccgt ccccgcgttg
    20341 ctgcttctgc tcgtgctcgc cgccgccgta tccgccgccg atatgcaggt gcgcgtctat
    20401 ctcgacggca aggaggacgc cgccgggctt caccccctgg ggctcgacgt ggtctggcag
    20461 ggaaaggact ttatcgagat catcgcgacg gccgacgagc tccgcgaact cgagggtttg
    20521 gggctgcgca cggaaatagt ccacggcgac gtcgccgcct tctaccgctc gcgtctcgac
    20581 cacgcgcgcg acatgggagg gtacaagacg ctcgcggaaa tccaggcgta catggatgaa
    20641 atcgccttct tccacgacgc gatcgtcttc ccgaaaaccg tgatcggata cacgctcgag
    20701 ggacgcccga tgtacgccat gaagatttcc gacaacccgg agctcgacga acccgacgag
    20761 ccggaggtgc tctataccgc ggccattcac gcccgcgagg tgatcacccc cgagacggtc
    20821 cttcggttca tggattacct gaccgacaac tacggcacgg accccgcggt gaccgacctc
    20881 gtgaacagcc gggagctctg gttcgtgccg gtggtgaacc ccgacggcta ctactacaac
    20941 caggtgatcg agccggacgg cggcgggatg tggcgcaaga accgccgcaa caacggcgac
    21001 ggctcctacg gcatcgacct caatcgcaac tacggctacg agtgggggta tgacgacgat
    21061 gggtcctcgc ccgttccctc cgacgccacc taccgcggaa ccggcccgtt ttccgaaccc
    21121 gagacccagg tgatgcggga cttcatcagc gcccacgagt tcgtgttcac cctctacttc
    21181 cactcctact cgaacctcgt gctctggccg tggggctacg accagatcta cacgccggag
    21241 gaaccgctgt tcaggatcct gggagattcc ctggcggcct acaacggcta cgagccgacg
    21301 ccgtcgtggg ggctctatgt cgtcaacggc agttccgacg actgggggta cggggagcag
    21361 ctgctcaaga acaagaacta cgctttctcg atcgagatcg gggggtactc cgatggattc
    21421 tggcccgacc cgagccggat cgaccagctc accctggaga accggggacc ggcgctcttc
    21481 ctcgcgcggg tcgcgggcaa cccgtaccag gtgatgccgc ccgggcagcc ggcggtctcg
    21541 gtcgacgaca ccgtcgatgc ctcgggctac agcgtgcagt ggagcctcgc cgacacactc
    21601 aacccggccg tgctctggga gctggtggaa ctgcagggtt tcgggcgcac gaccgatcag
    21661 gccaacgcct gggacaactt ctcctcgaac gggttctcca tttccggcgc ccgctacagc
    21721 tccgcgccca cgagcttttt ctccggggcc gccaacaatg ccgcccgccg gatccagtcg
    21781 gtcgaaacga tcgccgtgcg cgccagcgac gtgctcaccc tgcgcgtctg gtatgacatc
    21841 gagagcggct gggactacgc ctacgtcgag gtgtcaaccg acggggtggc gttcacgccg
    21901 atcgccggca ccatcacgac caacgacgac ccccacggct acaaccgcgg caacgggatc
    21961 accgggtcct cggggggctg gacgctggcg tcgttcggtc tcgccgtgta cgccgggcag
    22021 agtgtctacc tgcgggtgtc gtacgagacc gacgggtccc agttgggcga gggaatctac
    22081 gtggacgaca tcacgccggt cgaagggtac gcgagcgaaa cggtgatctc gtcggcgctc
    22141 accgacacct cgttcgcgtt caccgacaag ccgaccggca cctactacta ccgggtgcgc
    22201 gcgcaggacg cccagggcca gtggggtccc ttctcggcgg ctgtccccac cgtcgtcacc
    22261 gacggcgagt accagtgtgt cgactccgac ctcgacggtt tcggcgaccc gggctacccc
    22321 gataacgact gccccaccga caactgcccg gccacggcca acccggatca ggcggacggg
    22381 gacgccgacg gggtcgggga tgtctgcgac ctgtgtccga acgacgccca gaacgacggg
    22441 gacgcggatg gccactgcgg cgacgtcgac aactgcccgc tcattgcgaa cgccgaccag
    22501 gcggacgccg atctcgacgg ggtcggcgac gcctgctgct gcgcgctccg cggcgacagc
    22561 gacggcgacg gcgcgatcaa ggtcggcgac ctcaccatgc tcgtggcgtt tctgttccgc
    22621 ggcggcgccg ggccgggatg tcccgcgcac gccgacgtca acggcgacgg cgggccggcc
    22681 gtgtccgacc tcaccatgct cgtcggctac ctcttccggg gcggcgagcc gcctgcggcc
    22741 tgtccttagg gcgcgaccgt actgatgact tggaggaccg ggctccgcgc ggggcccggt
    22801 ttcttttttg cccggcccgg cgggccggcg cggggcgcgc ggtcggcgcc taggcctcgc
    22861 ccggccccac gaataggacg ctcttgtaga ggtcgcggcc gtaccgccgc attcgggaga
    22921 acaggggcag atcaatcttg aggttgcgcg cccggtgcgg gtcgccgccg tactccgcct
    22981 cgtagggatc gtggacgtag acgaactccc ggtcgaatcc ggtgaccacc acccagtggg
    23041 gagcccggtc gccgtggagc tggtaagtgc tgatgagcac gatggggatt ttgccgcgca
    23101 gcatttcgac cgcgatgtcg tcgaaggaga aatcatagta ggacaccggg atcccccggt
    23161 gataggcccg ctcgcggaga tcctcgtgga cgagcttgat gacgcgccgc ttgtcggccg
    23221 tccgcaccga agagaagaac ggggtctggt tcttcgaaag gagcacgcgg gccgagaatc
    23281 cgcggtcgac gacggcatgg gccatgccga acggtccggt cccgcccatg ccggaggtca
    23341 tgaaaatcat cgtcgcctcc cgccacaggg tcacttcgag cagccggtca tcctcgaggt
    23401 ccggccggtg gtacttcatg gccatgatga gagccgccgg gccgcaggtg aaggtgaggg
    23461 tctgggcgta gtaggggact ttcaggcgga gtctctccgg gcgccccgcc gtcaacgggc
    23521 gggtcatccg cacgccgtca gcaccgtcct cgtagtactt cgggaggcgg ccgacggcct
    23581 cgaagtggtt gcgccggtag agggcgatgg ccccggcgtt gtcgacgcgc acctcgagcg
    23641 acagagtcgt gcagttgcgc cactccgcct cctggcggca ggcgtccagc agccgctggc
    23701 ccagcccccg gccgcgcatg tcggggtcga cggcgatgtt atagaggcgc ccgaccgggg
    23761 ctcccttgcg ccagagcatg tacgcggccc ccacaatccg cccgccaagg tcgaccacga
    23821 acgtggtcgc gttggcttcc tccagcaaat actcgatctg ctcttcgtgg aagcggtcga
    23881 tggcgaaggc gcgttcttcg agcgccacca ggccggcgag atcgcggtgg gcgccgaggc
    23941 ggagacgggc ggcggagtcg ctcatggcgt cgtcttgccc tcgacgagga accggatcac
    24001 cttctcgtac aggtcccccg cggcctggtc ctcttcgccg gcgttgatgg tcgggttgtc
    24061 gttgacttcg atgaccgcgt agccgtcatc cacctgcttg aggtcgatcc cgtagaggcc
    24121 gtcgccgatg gcgcgggccg cggccagggc gacctccagg agctgcggat cggccttctc
    24181 gatcggcact cccttgacag ggccggaaat ggcgcggttg ttctcggcgt aggtcagaat
    24241 cttccagcgc ttgcgcggga tcgtgtactg gcacacgtag agcacttcgc cggcgacgat
    24301 gccgacccgc cagtcgaaat cggacttgat gaagcgctgg gccacgagcc ggtcagagcg
    24361 gcggaggaac cggttgccga cacggacaaa ggcctcgggc gtctccgctt tctccacgta
    24421 catcgagaag ctgctgttgg gcgctttgag aaccaccgga ctgccgagct gttcgaacac
    24481 ctgggccgcg cgcgcccgcg tgagatcctt ctcctccagg aagagcgtgt cgggcatggg
    24541 gaccccctcg cgctggaggt gggcgtacat gttgaccttg tcgcagcaga tgtagatcga
    24601 gcgcggatcg tcgatcaccc gcttgccgtg catttcggcc atgcgggcgg cgacgtacga
    24661 ggtgttgagc ggatcggtca gggcgcggat gaagatcgca tcgtactggg gaatcttggc
    24721 cacgtccgga cgaaagaggt agtccacctc gtgcccgatc ttgcgcgcca ccatggcgaa
    24781 gcgcatgagc gcctccatct catcggaccg ggtgatggtg tagcgctcga tgtagacgcc
    24841 tattcttccc atcgaatctt cttctccagg tactccagtt cgcgttgctt caggtccctg
    24901 tacagcagcg ggctgatctt gctcagcagg atgcggtcgt cgtaggtgcg cagcacccgg
    24961 acccgggcga gcgggatctg gaacacccgc cagatcttct ccgccgagtc gcggtagcgg
    25021 gcggcgacgc tccagccgag gacggcgcgg aagtgctcga tctgggcctc gggcgggatc
    25081 tcctgcacgc aggcggcgta ggtgaaattg cgcgtaatcg atttcgacac cgacttctgc
    25141 tgcgacatct tcagcaccgt ccggctccgc gacatgaacg ggttgatgga gtcgacgatg
    25201 gcgggcgctt cgaagtagcc gttggtcacg tagtactccg gcacgtcgag gccggccagc
    25261 ttggcccgtt ccaggaagag cggcacaatg taagcgtcga gcatctcctt gcacgtcggc
    25321 cggatgggga gccggctgtg ttcgtagtcc tgcgagacgt agtaggggag ttcgaggtac
    25381 tcgtagcgcc cggcgagatt gacgaaggtg tcgtcttcgt tgaggatggc gacgtagcgg
    25441 ttggccttgg cccgcttggt attcttggtc atgggcgcta ataacggcat tcacaagtcc
    25501 cggcgcaaca gtcaatctgc gacgaagccg gtggcgggta agcgattgcc ccggcccgct
    25561 ccgaaccgcc gctgtgcaca aaaacaccac cgccgccgaa gacgggcgcg gccgctcccc
    25621 tgcaaccccg cgcggtccgg ttgcacactc ccggccccgc cgcccgcgct caactgcacc
    25681 gccgaccggg gtttagccgc ccgctccggc cgggcacgcc ccttgcactc tccgccctca
    25741 tggactgcta cttcgggtcg attcatctgc cggaggaccg ccagctggac cagtcggtgg
    25801 tagtgtcgtt caagatcccg gagctcggga tcaagttcaa agcgccgttc gatgcggtgg
    25861 acgccaacca cggcgacctg gccgcgctgc tggcgctgct ggagttcatc gacagcaacc
    25921 agaagtattt cacgcggcac acctatcaga tttacgggaa taacctcaag gtcatcaacg
    25981 cggtcaaccg ccgggattgc ctccccccgg agttcgcgcc cctgatcgcc aaggccctgg
    26041 gataccggga caagtaccgc ttctcgctcg cctgggcccc cctgcgctcc aacccggagc
    26101 ttgaagagct ttttgattga cgctcacggg gcggtgatct atgttgggat cgccatgccc
    26161 agtccgagcg aacttctccg ggacatcaac gccggcgtct tcaaaccggc gtactacttc
    26221 tacgggcccg aggactaccg gatcatcgag gcgaccaagt acctcgcccg ccagtttctc
    26281 cccgaccgcc agctccagac caactaccgc aagctcagcg ggcgcaagaa cccggcccgg
    26341 gaggtgatgg ccgaactggc caacctcccc atgctcggcg agcgccaggt cttcagcatc
    26401 agcgacttcc agagctacca gccggccgac gtcgagcgga tcctcgggat gctccgcccg
    26461 cccgactcga cccgcgtcgt cgtcttcagc tccccctcgc cgcgcactcc caagaagacc
    26521 tcgaagtttt tcaaggcggt ccaggcggcc gggatcgtca tggtggagtt tccccggctg
    26581 acggccaagg aggttcgggc gcagatccag acgcgcctgc agaaagccgg tctgcagatc
    26641 gagcccgagg cgctccgcgt cctggtcgac ctggtggcgg gaaatctcgg cgccgtcgcc
    26701 gccgaaaccg acaaactggc caactaccgg gggaaggggg ggacggtcac agtcgatgat
    26761 gtgaccgcgg tggcggccgg ctacgagacg atcaacatct tcaccctggc cgaccagatt
    26821 gtcaccgaac agccacaccg ggtgctccgc agtctggagc agttgatcgc ggaggggaat
    26881 tcgccggtga cgatctgcac gcttctgagc acccacttcc tgcgcctcta tctcgtacgc
    26941 caccggaaac cggtgccggg gaacatggcg tggctggagt cccgcctgcg gccgcaggcg
    27001 aatcggttcg acagcgcccg gctggagcag attctgatcg atctggccga ggccgaggcg
    27061 caattgcggg gcggcggttt gcccccgcgc ctggccctgg aaatcctcgt ggtcggcctc
    27121 atgccgggcc gggacgcgct ctatgggtga aaattctcgg gaagaaaacc ttccccggcc
    27181 ggtatccggt ctggacaaag cggccgagcg ggccttggtc gaggcggccc agaacggcga
    27241 caataaggcg tttggacggc tcatccgcct gcaccagaaa cggctgttcc ggtacatcta
    27301 cgccatgctg gggtcgttcg atgccgcgga agatgttgtc caggaggcgt ttgtgaaggc
    27361 atacgaaaac ctgaaaagct tcaagcccga gtacgatttc tacccctggc tctctacaat
    27421 cgcccgcaac accgcctaca acctggtccg caaggaggaa aagaacgagt ccctgaatgc
    27481 cttagccgaa aagggtttcg accccgaatc gggcgacccc ggaccgctgg aaaaactgct
    27541 ggcggaagaa ggagagaaac ggttctacca ggctctcaaa actctccccg tgcagcaccg
    27601 cgtcgccttc gtgctccgcc acctcgaggg catgaactac gccgagatcg ccagctacct
    27661 gaaaataccg ccggggacgg tcgattcccg gctctaccgg gcgcgccaga tgttgatgga
    27721 ggcgctccag gacctcctct gaggtcaagg tacagcagtt gaattatgga tcacgggtac
    27781 tttaaggaca gagtctccgg ttatctcgac aaggaactcc cggactacga gatggaggcg
    27841 ctccgccgcc acctcggaga gtgcgccgaa tgccgcgccc tctacgagga tctgcgacgg
    27901 ctggaggcgc tggtcgagcg ccatgccgac ctcggcgggg acgaggacta ctgggaggcg
    27961 gcggccgccc ggatcgaaaa gcggatcggg atcgccggcg caccggaggt gatcgccctc
    28021 ccgccgcgcc ggagccgggg gctggcctgg aagctggtcg gagtggcagc ttcgatcgcc
    28081 gcgctgacat ttatcgcggt gaactacacc gacatcgtcc gccagagaga cgagtcgccg
    28141 aagatgtcct tcccgtccga gccgccgtcg gcccgtgact cagccgcggc cggccgagcc
    28201 ggggagccct caccggccgg cgaagagcag ccggcggaga aagccgagag aaccgcgccg
    28261 cccaccgcgg agccgcccga gccggccgag attgcggcgc tccctgagct caagcgccag
    28321 gcggcggagg acgaacaact cccggttatc gccgaggaag cacaggaagc gccgcgcctc
    28381 aaaccggccg gccaggagct tgcggcctcc ggcgggaagg cggccgcgca agatgccgag
    28441 gcggaggcga accggagtgc ggcggctccc gcggccccgg ccgcgccgcc gccggaaccg
    28501 gtctcggccg agacccgcaa agacgagggc gcggccgacg tccgcctcgc cgaccggctg
    28561 gtcctccgcg acctcatggt cgccaaggag ccggcgacag cggcggcgcc cagtctcagc
    28621 ctcgccgatt cctcggcggc cgatcggcgc ggacgcctgc ccctggcgca gtcgtcgaaa
    28681 gggttcgcgg ccgccggcga aagcgctccg gcggccactc tcgccgactg gcggaaccgc
    28741 cgcgattcgc tcgagtcgct ctggagcgaa ctgcggagcg aacatctgaa cctcgcgctg
    28801 cccaaggcgc gacgcgaccg ggcggcgctc ggggacatcg aactggtcga gcgccagctc
    28861 ctcgaggccc aggtgcaggt cggccgcctg gcggagggag cggatgctcc ggagcgcgac
    28921 cgggcgatcc agttcttccg cacctacctc gaactggccg acgtgcgcta ccgggagacg
    28981 gcgcgcctct atctggacga gttggccccc gataccgtcg acaccagcgg ttctcgccgg
    29041 taaccttttc cgccgattca cgtatagtat cccggaatcc ggctgcggcc ggtagaaagg
    29101 agacgatggc gcatgagcta caccagactc tatcggtcgg cggagaacaa ggtgatcgcg
    29161 ggcgtgtgcg gcggtctcgg cgaatacttc gacgtggacc ccaacctcgt gcgggtgttc
    29221 gcggtgctgt tgtttttcgc cacccagggg ttcgcgaccc tcgcctatgc ggcggcctgg
    29281 attatcgttc ccaaacgtcc gctcgaaggc gccgtcgagg aagttcgccc ggagcggccg
    29341 cgctcctcgt ggatccgcta tctcccaggg ctggtcctca tcctgatcgg ctcgctgcta
    29401 ttggtccggc agcactggca ctggatcgac tttgaccagt ggtggccggc cctcatgatc
    29461 gcgggcggtt tgtttctgct gctccgcccc aggcggcgcc gcgaagcctg ccggccggat
    29521 ctgtccgccg cgccggggga gatcccgggc ggcaatgaag ggaggacggc atgacaccgg
    29581 cccgcttccg ctggggactg ttcctcgtcc tgctcggact gctcctgctc ctgcgcaact
    29641 tcggcgtcct caacgacaac ttctggctcg acctgctcat cctcttcccg gtcgtgctta
    29701 tcatggtcgg cctcgagaag atcttcgccc gcacccgcct cagcatcatt tcctacctca
    29761 cttcggtggc gctgttcgtg ggcgggctgg cgatcgcttt caccagcagc cgcggcggcg
    29821 acagcacgag cttcttttcg accgcgacct acgtgaagga tgcggacagc gccgtgagat
    29881 tcacgcgggc ggtgctggat ctcgatgagt cggatctgac gatccgggat tcgggggatg
    29941 acctcgtgta cgcgaagttc gacaagttca cgcgcaagcc gcggatcgac ttccgcgagg
    30001 aggacagcct cgcggtggtg gaattcgcga gccgcccgca cgggctgctc ggcggcgcga
    30061 tcaagatcga gaccgacgag gcccccgact ggttcgtgcg tttcaagcgg gacgtgccgc
    30121 tcgaaatgcg cctcgccggc gttaagagcg acatgcacct caacatgtcg accacccccc
    30181 tccgggcgct tgatctctcc gccgaccagt cctcggtcta cctcaaaatc ggcgacctcg
    30241 aaccgttcgt gaaagtctcg gtgttcggcg aggataccga gctgcgcctg cgggtgccgc
    30301 gcacggtcgg gctgagagtg ctggccgggg aggagtacaa agcctacctc tactcgctcg
    30361 gcctgatcac cagggacgat gcctgggtca acgagggttt cgataccctt cccaacaaga
    30421 ttgaagtgaa gctcgacgac cggctgcagt cggtgtcggt ggatttcttc tgatcggttc
    30481 tttcctccag ttgtcggtga gatggcggcg gcgcttcggg gcgccgccgt tttccttgcc
    30541 tgcgtgccgg caggccgggg agaactactg ggggaagaag agggcggaga gatcctggaa
    30601 gaagtgcgcg atcacggccg gccagagcgt cccggtacgg atgtacagaa gcgagaagag
    30661 cgccccgtag acggcgatga cgatcacgcc gggcgccccc tggtaggcgt gcaggctgcc
    30721 gaacacgagc gcggacacga ctgtcggcat tagccacccc cgcgtgcgcc cgaggaggcg
    30781 gaggcgcgtc atgagatacc cgcgaaaggc cgtctcctcg accacgccgg cggtgatcgc
    30841 gaccacgacc cacacgatgc gcccgaccag gtcggtcggg atgaggaacc ggatgtcgcc
    30901 cgacatgggc agcccgatct gcccgagcac ccaggcgaga ccggagagca ggaggttggc
    30961 ggccagcagg aaggcgaccg cccaggcgag atccaccggc cggaagcggc gaaaccccag
    31021 ccccgccagc ccggtcttct ccgactccgc cgccagcacg agcaccacga acagccccca
    31081 ctgaatcacg atcgtgacca gcagcatcag gatgcgcatg gtgtcgttca ggcccttcag
    31141 tgcgatcgag ggatcctccg cgaatgcgat cgacaccgcc gggtagatga ccaccagcgc
    31201 caagagagcc accgcggtca gccaggtgcg gcgaaactgc gcgttgcccg aggccgcccg
    31261 gaagcgcctg acgtggtccg gttcggggtt cgggtcatag accggtatgc gttcgtacgg
    31321 ggtttcttcc acgccaccta ctttccgcgc cgcgacgggg tccggcggac ggggcgcggc
    31381 tcgccggagt atttcggccc tggtcactgt atcgccagaa cagccggccg gcttgacctg
    31441 acgcccggcc catccggcga tgcccgtcaa gatggtcggc cgaaccggca gaggcaacgg
    31501 aaaagtgaat cacatgagag cggcggcgcg ggcgggacag gtccgatgtg atgatcacgt
    31561 ggtcgcgaag cgctgccgga ggctgcatct tgaggcggag gaacaacatg cgcaagagca
    31621 gactgatcta cagcaacggc atcgtcggcc tgggacggga tcccggccgg ccgcccgatc
    31681 cgccggcgga cacacccgga ccggcgctgc cggagccgcc cgaggactcc gccgcgaccc
    31741 gggccgtgac cgccgctctc gcggccctgt ccgacgagga gcgggaattc gtgcgctgct
    31801 tttactacat ggggtgtacg gttgcccaga ttgccggacg gaccgggcgg gccgagcatc
    31861 ggctggaggc gctccggcgg cgcgtggagc ggcggctgcg ggctcgcctg ggaccgttcg
    31921 tccgggcgcg ctacggcata ggcgccgatc ccggccgggc ctgcccgatc tgcggcgcag
    31981 cccggcggga ggcgatcgag caggcgatcg cggcaaagcg gcccgaggag acctggggac
    32041 cggttctgcg ccaactgcgc gagcggttcg ggctgccgtt gcgctcgccg cagacaatcc
    32101 tgagccacgt caaattccac ccgaaaccgc cggaccgccc cgcggccgac agtggcacgc
    32161 aggatacccc gccgaccccg gacatgaacc aaccaacgga tggagcacac agatgagcga
    32221 aatgcgggag aaacccgagg gcagccgacc gccggcgccg gaagcggcgg cgcggacgcc
    32281 gcgaaaccac accatccacg tgttcgtggc ccacgacctc aaagcgcggc tcgaagcgct
    32341 ggcggccagg tacgaccgga ccacggccga catggtgcgg gcggtgctgc ggatcggcat
    32401 ccccatgatg gaaggcctgt cggaggccga ggaggtgatg gtgaaggagt atatcgagtt
    32461 gttccgcaac ctccggcagg tgaagtcgct gcgggatatc taaggccgag ccgggcgcgc
    32521 acgtttcagc ttttccgcgg ggcgtgaaat cgctatactg gcggcgtgat tggaactgct
    32581 cgacgagaac atcgcgagaa ccggagagtg cagcgatgag cgaacgatcc ctgggccgga
    32641 gggcggcttt gaccgccctg tgtctggcgt tgctggcggg ttgcgggagc gacccggcgc
    32701 cgcgcccggc cgatcaggtg gaggcggcgc tggccgcggc cggcgccaac cgcggcgaac
    32761 tggaaaaagt gctcgcccat tttgacggca agggggacac gttgatgctc gacgccgccc
    32821 ggtatctgat cggcaacatg gaagggcaca gctacgtcac ctacgccctc atcgacagcc
    32881 tcggcgacac cattccctgg gatgccgcgc ggtttccgga ctacggcgcg ctacaggcgg
    32941 cgtacgggga gctggagaag gcccgccccg gtctggattt caaaagcgcg gtggcggcgc
    33001 gggacctcga gacggtcacg gccgactacc tcatcgagaa tgtcgagctg gcgttcgccg
    33061 cgtggcggac caaaccgtgg gcgaggaagc tctcctacga ggagttccgc gaaggggtgc
    33121 tgccgtaccg ggggagcaac gagccgctcg aaccgtggcg ggcggagctg atggcccgct
    33181 acgccgacct cgcgggcaaa atgacagact caaccgaccc ggtggaagcg gcgcggctga
    33241 tcaacgacga catcaagagc tggttccgtt tcgacgagcg ctggtatttc caccccaccg
    33301 accaggggct ggccgaaatg aaaacgaccg gccgcggccg ctgcgaggac atgaccaacc
    33361 tcacgatcta tgccatgcgg gccaacggac tcgccgtgac ctcggactac accccctact
    33421 gggcgaacgc gggcaacaac cacgcctgga acgccctcat cctgccgggc gggaaagcca
    33481 ttccgttcat gggcgccgag gccgaccccg gcgagtacaa cttgatccgc cgagcggcca
    33541 aagtgtaccg gaaatcgttc gcccgacagc cgcggaacct cgccttcgtc aaggaagaaa
    33601 accgcaaagt gcccggctgg ctggccggca agagctaccg ggacgtcacc gcggcctacg
    33661 gggaggtgtg cgatgtggcg gtgacgttcg ccgaggcccc cgcggattcg gtccgcttcg
    33721 cctacctgtg cgtgttcaac gacggcgagt ggaaaccgat ccagtgggcc gagatcgccg
    33781 gccgacgggc ggtcttcgcc gccatggccc ccgaggtcgt ctacctcccc gggttctacc
    33841 tcaacgaaga gatagtgccg gccggagagc cgttcctcct cgagccgggc ggggttcagc
    33901 aggcgctgac ggcggagctc gccgagccgg tcgacatcgt ctgtcgggcg atcatgagca
    33961 aagacctcga caaggcgtcg gaaaacgcct ccggctcccg gctgatggcc ggggtggact
    34021 atgagctgtt cttctggggc ggggaatgga agtcgcttgg gacggcgacg gcggccgaag
    34081 aaccccttgt gttcctcgcc ccctcgggcg ggctctaccg gctcgcggcg gcgggctccg
    34141 acggggacga gcggatcttc acgtggcgcg agggagcgca gacgtggtgg tgagcgggcc
    34201 ggcggggggg cgatcgtgac cggtccggaa ctctcgccgg gcgggatttc cggcagcctt
    34261 cacaggtgtt cacacttacc gggcagcgag ggagcagact atgggcgagc cggcaggcac
    34321 gcgacttgac gttgacggaa tcatgaatcg ggcggcggcc gcggccgagg ccttccgccg
    34381 ctgcgaccag gagcataccg accgcatcgt gaccgcggtc taccgggccg ggttcgacca
    34441 gcgcatcccc ctggcgaaaa tggcggccga ggaaaccggc ctcggagtgt ggcaggacaa
    34501 ggtgatcaag aacgtgatcg ccacccggtt tgtctacgag aacatcaaga ccctgaagac
    34561 ggtgggggtg attgccgaga acgaggaagc ggggatcgtc gagatcgccc agccgatcgg
    34621 accgatcttc gccgtcaccc cgatcaccaa cccgacctcg accgtgctgt tcaagatcct
    34681 cattgcgttg aaagcgcgca acccgatcgt gatccgtccc cacggggcgg cgcggaagtg
    34741 ctcgatcgag gcggcgcgca tctgctacga ggcggcgctc gcggcggggg cccccgagca
    34801 ctgcgtccag tgggtgcgcc ggtcgaccga agaggaaacg ctcgccctca ctcggcaccg
    34861 caagacggcg ctgattctcg cgaccggttc ggaggcgctt gtccgggccg cctacagctc
    34921 cggcaacccc gccatcggcg tcggtccggg aaacgtgccg gtgtttatcg gcaagacggc
    34981 cgacgttccg ttcgccgtcg accagatcgt catgtcgaaa actctcgacc acggcacggt
    35041 gtgcgcgagc gagcaggcgc tcgtggtgcg agcctgccac gccgccgaga cgcggcggct
    35101 gctcatcgag aggggggcgt acttcctcac cccggaggaa atccgccggc ttgaaacggt
    35161 ggccttcaac ccggccaccc gcgtcatgcg ggtcgaggtg atcggccagc cggccgagaa
    35221 gatcgcgcgg atggcgggga tcgaggcgcc cgccgggacg aggctgctcg tcgccgagct
    35281 tgaggatgtc ggcccccaca cgcctctctc gctggaaatc ctcgcccccg tcctggcctt
    35341 ctacgtggcc gaggatatcg accaggcgat cgccctctgc cgcaaaatca accacaacgg
    35401 gggactgggc cacaccatca gcatgttctc aaacaacgag gagcggatcc gctacttcgc
    35461 ctccaccatg aacgccgggc ggatcctggt caacacgccg gcctcgcagg gggtgctggg
    35521 cggaacctac aacgccctcc agccgtcgct caccctcggc tgcgggaccg gcggaaagaa
    35581 catcaccacc gacaacatct cggcccgcca cctcctcaat atccagcgga tcgcgcgccg
    35641 ccgcgtcagc ccgtgcatcg gcccagagat gatggcggcg tacctggacg agacggtcga
    35701 tgccctcgcg ctggagcggc ggtgcgcgga aatggaggcg ggcgccgcgc ggcccggcga
    35761 ccccgccgga gaaccgccaa aaaagacttg agccggttcc tcctttcttg tatcatcgac
    35821 caaactacga tcgagtaacg gaagtatgta cgagaagcaa cggaccatca ggcggcctat
    35881 ttctttgtcc ggcatcggat tgcataccgg caacctgtgc acgatgacgt tccatcccgc
    35941 cccccccgac cacggcatcc gcttcgtccg cgtcgatctc cccgaccgcc cctcggtcat
    36001 cgccgatatc gaccacgtcg tggatatctc gcgcggcacg acgcttcagg acggcgaggc
    36061 gaaagtccac acggtcgagc acgtgctcgc tgctttcgcc ggcctgcaac tggacaacat
    36121 gcttgtcgag ctggacaaca acgagccgcc catcggcgac ggttccgcga aaccgtatgt
    36181 cgacaagatc ctcgaggcgg ggatcgagtc acaggatgcc gacaaggtgt acctggagat
    36241 cgacacgccg atggcgtaca acgagcggga gcgcggggtc gacctggtgg tggcgccctc
    36301 gaacgacctg cgcatcacct tcctcatcga ctacaagaac ccggctctgg ggacgcagta
    36361 cacgacgctg caggatctcg agcaggagtt tgtcgatgaa ttcgcaccgg cgcggacgtt
    36421 ctgttttctc tccgaggtgg aatcgctcaa ggccgccggg ctgatcaagg gggggagcct
    36481 ggacaacgcg gtcgtgatct acgactccga ccgcggccag gtcgaggtgg accggatccg
    36541 ccgggcgctc gatttgaagg aggaggcatt cgtcggaaag accgggatca tcaacgacat
    36601 tcccctgcgt ttctacaacg aaccggtgcg gcacaaggcg ctcgatctac tcggcgacct
    36661 gttcctcatc ggcgtgccgt tcaaggggca cgtgctggcc gcccgctcgg ggcacaaggc
    36721 caacgtggcc ctcgcgcgca agatgcgcga cctgtacaag aagaagaaga ttgcgagccg
    36781 gtacgccggg cgggcctcgg ccgcgctctt tgacatcgac gccatccagc ggatcatgcc
    36841 gcaccgctac ccctttctcc tgatcgaccg catcctcgat ctcgagccgg acacgcgggt
    36901 gagcgcgctg aagaacgtga cgatcaacga gccgtttttc caggggcatt tccccggcca
    36961 ccccatcatg ccgggcgtgc tgatcctcga ggcaatggcg caggcgggcg gcgtgctcct
    37021 gctcaacgcc atcccgcagc cggaaaccaa agtggtgtac ttcatgtcga tcgacaacgc
    37081 gaaattccgc aagccggttg tgcccggcga ccagctccgt ttcgagctcc agatgatctc
    37141 gttccggcgc atgacctgca agatggcggg cgaggcctac gtcgacggcg agctggtggc
    37201 cagcgccgag ttcctcgcca tggtgaggga ccgatgagcg gcatccaccc gaccgcgatc
    37261 atatcgccgg ccgcggagct ggccgaggac gtttcggtcg gaccgtacac ggtcatcgag
    37321 gatgatgtgc ggatcgggcc ggggacggcg atcgcctcct cctgcctgat cgcctcgggc
    37381 gcccgcatcg gcgagcgctg cgtgatcgcc cacggcgcgg tgatcgccac ccagccccag
    37441 gacctcaaat tcggcggcga gaaaacgttc gtggagatcg gcgacggcac cctcatccgc
    37501 gagtacgcga ccgtgaaccg gggcaccgcg gcccacggaa cgacgacggt ggggaagggg
    37561 tgcatgctga tggcctactc ccacgtcgcc cacgactgca tcctcggcga ccacgtgatc
    37621 ctggccaacg cggtgaacct cgccggacac gtggagatcg acgactacgt catcatcggc
    37681 ggcatcgtgc ccgtccacca gttcgtgaaa atcggggcgc acgcgatgat cgggggggga
    37741 ttccgcgtgc cccaggacgt ctgcccgtac agcctgtgcg gcgggtaccc gctcagggtc
    37801 atgggcatca acgccgtcgg cctgcgccgg cggggcttca cccgcgatgc cgtcgccgcc
    37861 atcgaacagg ccttcaaaat cctcttcttc agcaaactca acacctccca ggcgctggaa
    37921 cgcatccggg cagaaatgga gatcacgccg gaggtcgggc gcatcctcga gttcgcggac
    37981 cgctcgaccc gcggcctgac gaaatagagc aaacgccaac ccgtcagagg ggagagtggc
    38041 ccatgtctca aaccgcagtt gtcaccttcc gcgcggcgaa ggaaaccgag tacgcagggt
    38101 ttctggatct gatccaccag cacgccgccg attacctgga accgtcgctc acgctgctcg
    38161 gcctggacaa gaccgagttc gcccatctgt tccgcacgcg gggacaggtg gtgagtattc
    38221 tcgccgaccg cgccgtggcc gggttctact ggattgagga gcgcgatggg accctccaca
    38281 tccatggact ggtgatcaag ccggagtttc agaagcgggg cctggcggcg gccgctctgg
    38341 cccacctcga aacgggtgca tcgcccgacg tggcccggtt cgagctgggc gtgcaccgcg
    38401 ccaacggccg cgcccgcaag atttatgagc gggccggcta ccgggtgacg cgggcgcttc
    38461 ccgacttcga tttctccatc atgcacaagg accgtcccgc gcagagctga agggccgccg
    38521 cggccgacag cgccgcgggg aacaacattg tccttttgcg gcgccgccgt ttgtattact
    38581 ttcccgtcga tcgcaaacga cggcataccg ccgcccggcc cgggcgggcg cgcggcggcg
    38641 cggaaccgtc acggacaggg aaagcagcag ctatgacacg gattcggacg ggcgtggtcg
    38701 gtgtcggcgc gctcgggcga caccacctcc gctggttctc gcagatcccc gagtcgcacc
    38761 tggtcggctt ccacgacatc gacccggaaa agcgggagcg ctacgccgcc gaatacgggg
    38821 tggcggcctt cgcgacggtc gaggagctgg cggaagcggt tgatgcggcg agcgtcgccg
    38881 tgccgaccac cgcgcaccgg gcggccgccg gcgcgctttt gaaacgcggg gtgcactgcc
    38941 tgatcgagaa accggtgacg tcgacgctgg acgaggcgca cgagctggcg gaactggccg
    39001 cggccagcgg cgcccgggtg gcggtcggac acatcgaacg gttcaacccc gccgtgcagg
    39061 cggtgctccg catcggcgtc cggcccgcct tcatcgaagc ccaccgcctc gccgccttcg
    39121 atccccgcgg caccgacgtc gccgtcgtgc gcgacctcat gatacacgac atcgacctcg
    39181 tcctgacgct caccggcgcc cgggtcgtcg acatccaggc ctcggctgtc gcggtcgtgt
    39241 ccgatcagcc cgacatcgcc aacgcccggc taaccctgga cagcggcgcg gtggccaacc
    39301 tcacggcctc ccgcatttcg ctcaaccaga tgcgcaagct caggatcttc cagcgctccg
    39361 gctatttctc gctcgacctt gcgaaaaagc aggccgacct ctaccgcctc gcggcgccgg
    39421 gagaggaaat cgagggactc agggtaccgc tcgggaaatc ggggcgagaa ctcatctatg
    39481 cctcgcacgg cgacaccggc caggaccagc tccagatgga gctgagcgcc ttcctgcggg
    39541 cgcttctcga cggaggagcg atggcggtgc cgctcgccca gggaatcgag gcgctccggg
    39601 tggcgctgga ggtggagcgg accggccgcg cgtcgctcgc gcgcatgctg gccgacgggt
    39661 aagtcgcgcc ctatggaatt gtttctctcg gccggggacc tctccggcga caacgccgcg
    39721 gcgcggctgg tggcggcgct gcggacgcac gatccggggc tccgcctgtg cggactgggg
    39781 cgcacacggc tcgcccgtct cggccaggag cagttcgcgc ccggcggcga cctcgccgtc
    39841 atcggcttct gggaagtggc caaacggctt ccctatttcc ggcgcctgct cgctcgctgc
    39901 gccgatgaga tcgcccggcg cgccccgcgc gccgtcatcc ttgtcgacta cccgggtttc
    39961 aacctgcgcc tcgcccgccg ggtgcgccgc ctcggcatcc ccatcatcta ctacatctcg
    40021 ccgcaaatct gggcctgggg gtcgaaacgc ctgcgcgaaa tcgaacagct cgtcgatctc
    40081 atgctggtca tcctcccctt cgaggaggac ttctaccggg ggagccgcgt caacgtgcgc
    40141 ttcgtgggac actacctgct ggaggacatc gcggcggaga tgatcgcctc gccgccgccg
    40201 gagagaatgg cgggcggcct cgcgctcctt cccggatcgc ggccgcagga gatcgaccgc
    40261 ctgctcgggc cgatgctcga gactgcgcgc cggttcacgc gcgcgcacgg cacgcgggcg
    40321 gtggtcgccg ctctcgcggg agcgtatccg tacgacgctg ccctgcgcgc ggaggatcac
    40381 gcggcgatca cggtcgccta cgacgatgcg cgccgcgtca tccacgacag cgcgctcgta
    40441 ctcacggcct cggggacggc gaccctcgag gcggccgtca tcggccggcc catggtcgtg
    40501 gcctaccgca ccggcgagat cacctatcgg atcgcccggc ggctgatcac acttgacaaa
    40561 atcgcgctgg tcaacttggt tgccggacag gaggtggttc cggaactggt gcagcacgag
    40621 gcgacccccg accgcatgac ggcggcgctc gagcgcctct ggaacgaccg ggaactctac
    40681 cttcggacgg cggccgccct gggccgcatc cccggtctgc tcggcgggac cggcgcctcg
    40741 gaccgcgccg cccgcctgat catcgaccac ctcgaaggaa agtgattgcg tgctctcgct
    40801 ctaccgcatg ttcacccggt tcatccatac cgtcagctat cccgtgaccc gctttcgggc
    40861 cgcgcgcggc gagcagatct ggcgcgatcg gctcggcctg gacaccgcgg gcatcgaagc
    40921 cgagctctgg ctgcacgccg cctcggtggg cgaggtcaaa gtgctgtcct gcctctaccg
    40981 ctatctcaaa cggcggcggc cgcacctgcg ggcctgcgtg accgtcacca ccacccccgg
    41041 gcaccagacg gcgcggaagt tgtttggcga ggaggcggcg gtgcggtatc tgccggcgga
    41101 caccacgccg atcgtcgcgc gcgtgcttgc ggccgtgcgg ccgcgctgcc tcgtgatcgc
    41161 cgagaccgaa atctggccca tcctcattac gcaggcggcc cggcggcgca tccccgtcat
    41221 tctcgtcaac ggacggatgt ccgacaccgg cttccgccgc taccggatgg tcgccccctt
    41281 cctccgcgga ttgctcccct gctacgaccg gttctttttc aagtccgagc aggacgccga
    41341 gcggtaccgc tatttcggcc ttcccgagag cgcctgccgc gtgaccgggg acatgaagtt
    41401 cgatgccccg ctgtggccgc ggtcggcggg acggcgggcg gcggtgcggg cgaccgtcgg
    41461 cgcgcgcgac cacgactggc tgattgtcgc gggttcgacg cgcccgggcg aagaggccct
    41521 gctcctccgg gggataccgg acctgcgcgg cgcgggcaac ggtctgagac tggtgctggc
    41581 cccgcgccac gtggagcggg ccgaggaggt cgcgcaggcg tgccgcgacg ccgccgtgcc
    41641 ctgcgccgtc tacgggagcg ccgacctgtc ggccccggtc gtgatcgtcg accgcatggg
    41701 cctgctcatg gatctctaca tggcggccga cctcgccttc gtgggcggca cgctggtcga
    41761 cgtgggcggg cacaatatcc tcgagccggt gtgggcgggc acccccgtcc tgtacgggcc
    41821 gtcgctggcc aacgtcggcg acgccgccga gtacatcgag gcgcataact acggcgcccg
    41881 ggtcggcggc cccgaggagt tcgcggcagc cgtgcgcgac atccgggccg gccggcggac
    41941 tttcgcaaca aaaaccgaac acgactacga ccggtcgccg accgccgcgg ccggcgagta
    42001 catattggcg aagctgtccg atgattgagc gatgctggaa gcgctttctc cgccgctcgg
    42061 gcatgtcgcc gtggggactg ctcggccctc ccctgtggct ggcctcgctc ctctaccggt
    42121 ttgtccccgg cctgcaccgc cgcctgacga aggagcgggt gcgcctctcc gtgcccgtga
    42181 tcgcggtggg gaatatcacg gtcggcggaa cggggaagac gccgatggtg cacttgctgg
    42241 cccggcggct gattgacgag ggcttccgca tcggcatcgt ctccgccggg tgggggcgcg
    42301 ccggtgacga gccgattctc gacgagggga ccggcctgca ggaccgctcg gccgacgacg
    42361 tcggcgacga ggttctgctt ctggctcacc tggtccccca ggccgtgttc tccatccacc
    42421 cctccaagag cgagggggcg cggcggctcg ccgcgtgcgg tgagcgtctc gacgccgtca
    42481 ttgtcgacga cgccttccag cactaccgcc tcgcgcgcga cctcgacctc gtgacttacg
    42541 acgccgccct gcccgcccgg gtgttgcgcc cctttcccta tggcgtcatg cgcgagccgc
    42601 gctcggctct gcggcgcgcg gacatcgtcg tgatcacccg cgccaacttc gcccgcgaca
    42661 tcggacggct gagccggcgg ctgtgcaaaa tcagcccgcg cgcggcccac tacaccgccc
    42721 agttctctat cagcgggctg gtcggcgccg agcggcggta cccggtgaag tatctggagg
    42781 acaagtcggt gttcctgttc gccggcgtgg gcaatttcga gcccctccgg cgccaggtgt
    42841 cggtgctcgc cggcgatctc gacgccgcgc ttgaactgtc cgaccaccag cgctacgacg
    42901 ccgcgacgct cgcccggatc aagcggctcg ccgaccggca cgactcggac gtgatcgtca
    42961 cgaccggcaa ggactgggtg aaaatccggc actttgattt cggccgcgag ttgtattatc
    43021 ttgcgcaatc ggtggacctc gatcccggcg aggagcagtt gatcgccgcg atcgaggacc
    43081 gcctgaacct gcgaaaggcc ggccactgat ggaacgccac tacgacttcc tcgtcatcgg
    43141 cagcggcatt gccgggctct tctatgccct cgaggtgctc gagcggcatc cgcaggcgcg
    43201 cctcgcgctg gtcaccaaga agcgggagtc ggactccagc accaaccgcg cccagggcgg
    43261 gatcgcggcc gtcctctccg gcgccgattc cttcgagtcg cacgtcgccg acaccctgga
    43321 ggccggggcg ggcctgtgtc gccgcgacgt ggtcgaaacg atcgtgcggg aggcccccga
    43381 cgcgatccgc gacctcgtgg agatcggcgt gcggttcacg cggacggccg aggggtacgc
    43441 gctcggacgc gagggcggac accgggcggc ccgtgtggtt cacgccgacg acctcaccgg
    43501 acaggagatc gaacgcgccc tcctggcggc catcaaggag cgccgggggc agatcgacgt
    43561 gtaccgcgac cacatcgccc tcgacctgat caagggaacg gccgacggca cgaccgtgtg
    43621 cgccggcgcc tacgtgttct ccgagaagga ccacagcttc tacgccttct acgcgcccgt
    43681 caccctgctc gccacgggcg ggctcgggca ggtctatttc cacaccagta acccgaaaat
    43741 cgcgaccggc gacggcgtgg cgatggccta ccgggccggc gtgccggtcg ggaatctcga
    43801 attcatccag tttcacccga ccatcctcta cagccccggg cgctggccgt tcctcatttc
    43861 ggaagcggtg cgcggggaag gcggggtgct caaagcggtc gacgggcggc tgatcatgga
    43921 cggcgcccac gagctgaaag acctcgcccc gcgcgatatc gtcgcccgcg ccatcgataa
    43981 ggaactcaag cagagcggcg aggagtatgt cctcctggac atcagccacc gcgacgcggc
    44041 ctttatcaag aagcgctttc cgaacatcta caagcagtgc ctccagcgcg ggttcgacct
    44101 cacccggcgg ccgatcccgg tggtgccggc ggcccactac gcctgcggcg gggtggtttc
    44161 ccgggtcagc ggcgagaccg cgctcgccgg gctgtacgtc gccggggaag tggcgatgac
    44221 cgggatgcac ggcgctaacc ggctggcctc caactcgctg ctcgaggcgg tggtgatggc
    44281 caagcgcgcg gccgcggccg cgtgcgccta cgccgaggct gccccgcccg cgccccacgc
    44341 cgccatcacc cccgacttct actcctccct ccagtacccg cgcgagaagg tgctgctcgc
    44401 ccacgaccgc agccgcctca cccgcgtcat gtgggacttc gtgggcatcg tgcgcaccga
    44461 gaaccgcctc cgcctggcgc tcgaggaggt ccagcgggtg aagaacgccg ttgagcagta
    44521 ctacctggcg acgccggcga cctaccaggt cgtggaactg agaaacatgg cgaccgtggc
    44581 ggaactgatc gtgcgctcgg ccctcctgcg caaggagtcg cgcgggctgc actacatcga
    44641 ggactacccc gctcccgccc ccggcgcgcc caccgatacg attatcacca atccccatgc
    44701 gcaaggatag gacaccgtgg cggaaccgca cgacatgatc ctcattctcg atttcggctc
    44761 gcagtacacg cagatcatcg cgcggcgcgt gcgcgaagcg cacgtctatt gcgagatcgt
    44821 tccctacaac catgacctcg gcgcctaccg cggccgcaac gtgcgcggct acatcctctc
    44881 gggcgggccg gcctcgctgt ccgatcccgg atcgccgcgg ctggccccgg aattctacga
    44941 caccaacctg cccgtgctcg gcatctgcta cggtctgcaa ctgttggccg agcggttcgg
    45001 cggacggttg gtggcctcga gcacgcgcga gtacggccgc tcgcgcctca gcgtcgtgga
    45061 ggccgacgat ctcctggccg gcattccggc gacttcgaac gtgtggatgt cccacggcga
    45121 ctcgatcgcc gttatgcccc ccggtttccg catcatcggc tccaccgaga cgctcgcggc
    45181 ggcggccatc gcctggcccg agcgcaaact ctacggcgtg cagttccacc cggaggtcca
    45241 ccacaccgac gagggacgcc gcatcctcga caacttcctc ttcgacgtct gcggcgtcaa
    45301 aggggactgg accaccgagg cctttatcga cagcgccatc gcccgcatcc gcgcccaggt
    45361 cggcgacggg aacgtcgtcc tcggcatctc cggcggcgtc gattcgacgg ttgcggcgat
    45421 gctcctccag cgggccatcg gcggccgcct ccacgcggtc ttcgtcaaca acgggctcct
    45481 ccggaagaac gaattcgagg atgtcaccgc catgctgcgg acgctcgaca tcaacctccg
    45541 gcccgtcgac gcttcggccg agttcctcgc ccggctcgcg ggcgtcatcg acccggaggc
    45601 caagcggaaa atcatcggca acaccttcat cgacgtgttc gaagcggccg cccggaatat
    45661 cggcaacgtc ggcttcctcg cccaggggac gctctacccg gacgtgatcg aatcgacctc
    45721 gttccgggga ccgtcggcaa ccattaagtc ccaccacaac gtgggcggcc tgaaagagcg
    45781 gatgcacctc aagctggtcg aaccgctgcg ggagttgttc aaggacgaag tgcggcggct
    45841 cggccgcacc ctcggtctgc ccgagcagtt catccggcgc cacccctttc ccgggccggg
    45901 gctggcggtg cggatcctcg gcgaggtgac gaaggaacgc tgcgacctgc tccgggaagt
    45961 cgacgcgatc ttcatcgagg aactgcacaa cttcaacatc tacgatgata tctggcaggc
    46021 gttcgcggtg cttttgctga tcaaggcggt cggcgtgatg ggggatgagc ggacttacga
    46081 ggacgtgatc gcgctgcgcg gggtgacgtc ggtcgacggc atgaccgccg actgggcccg
    46141 tatcgatccc gacatcctcg cccacatttc caaccgcatc atccgccacg tcaaaggcat
    46201 caaccgggtg gtctacgaca tttcgtcgaa accgccggcg accatcgagt gggaataggc
    46261 ggcggccgac cggccgccga acggaaaacc ggacgtggtg cgcatgctct catggctggc
    46321 tgttatcctc atcggcgcct ttgtcgccgt caacatcggc ctctacttcg cccaggggcg
    46381 aattgtcttc taccccagcc gcgacttcgg cgccaccccg cgcgacgccg ggatcgcggc
    46441 cgaggacgtc ttcatcgagg tcgtccccgg ccagcggatc cacgcctggt acgtcccccc
    46501 gcccgaaccc gc
//
LOCUS       DAOPOE010000014        41222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1266_length_41222_cov_6.222022, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000014 DAOPOE010000000
VERSION     DAOPOE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 41222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_41222_cov_6.222022"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKM94_03495"
     CDS             complement(<1..679)
                     /locus_tag="PKM94_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD65733.1"
                     /translation="MRLLTTRRFFATLAIIGAYIVYSLAFGSAAGAQGDQNPPAAQAA
                     TGAEQPAAPGPAAGVPVAHGEQSGSGHASEVLSVLLELIIIILAAKLGGDLFERIKAP
                     AVLGELVLGMLVGNAHLIGIVAFQPYLHDPNLNMIFQVLAEIGVMVLLFEVGLESTVR
                     EMMQVGVASFLVATFGVIAPFFLGWGVAAWFIPEESIYVHIFIGATLTATSVGITARV
                     LKDVGKVH"
     gene            1468..3609
                     /locus_tag="PKM94_03500"
     CDS             1468..3609
                     /locus_tag="PKM94_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD65734.1"
                     /translation="MFQEAEALLSQNRFTEVIALLDRVPPQSLSTEERGYCCILKSEA
                     MLYLGLDPGDLIDQASEVFRMHAEAEKFARAKLLKGWYLTTIGRYADSREALLEAYAN
                     YLRCRHKRGAARALNRLAFVHRQLGHYRAAEENLTRSAEIYRELADEISMTGVLTNRA
                     YLLMSGGSLRCAETEYSKMRDTVVQAGEKNVVAHGNMSAMCYALLGDLTTARRTIDEG
                     KPYLDTYIREKAIYHENLGLIEILSGNYEAAEQSLQTGLDISLQIAPESALVSQIKRL
                     FADLYIQTQHWTKAEKFAREALAVAEQINERLEIAACWRVFAQVENHRGNHDQARAWF
                     DQAIDLFNQIGSRYELAVTRCLAALSNLYDRNKRVALLYLAREYFESEGVAPWLTRVT
                     EALENEAPPAPRARPAAAAAVIVGADKSLRQILALAEHAAPTDFTVFLTGETGTGKDL
                     LARHIHACSGRKGEFVAVNAAAIPTDMIEAELFGHVMGAFTGAAADRRGLFEQAAHGT
                     FYLDEIADASPLFQAKLLQVLESRQIRPLGQNGLRPLTCRFVAATNHDLHDLLKSNAF
                     RPDLFHRLNHVPIHLPPLRERLSDIPLLVEHFLKEDGIVARSRNETLALERLCRLLAA
                     RSWPGNVRQLRSVVLRLAMIGGRDLPRMVEAARADIDQPCEAQDELLYMLQTTGWNRR
                     ETARRLGVSEATVRRRIAEFDLAVQIPQPPA"
     gene            4440..5630
                     /locus_tag="PKM94_03505"
     CDS             4440..5630
                     /locus_tag="PKM94_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65735.1"
                     /translation="MRYRSRLALFGLGCMALLWATLALATPPGVFLPFALDLRIVSYD
                     QSTGRVVLDVGIATHVPCPDGLSARVDSLDGLAYFGPDSWQLAMEPGGETLFTRVEFT
                     IPTHDTSGFELITSCGEHVYDWSRYFVTAGDSIAIVCGDPRTWPDPDPAPDSVVRRLV
                     WEEIERQLAEQRRTSPPPPPEGYSSTYIIIYPGDSALADSLTDDGKRRLAIMRSRERE
                     PLVDSDMDTWEIEGRVFQRAKGERQFHRIKGHTTEEWCELAVRMWDSLDAADADKPLE
                     IFVDLRKPEQEAFMRSFVDSLIPTDSAGIYRTVILYRQQKDLEAHGIRYRNASAGMWR
                     QRSRQPVGNPSPESREQEAPGKSADSGRSSMFYEDFEWGSFSNTWIAMDTLPQNGLDY
                     WGSI"
     gene            5685..5987
                     /locus_tag="PKM94_03510"
     CDS             5685..5987
                     /locus_tag="PKM94_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65736.1"
                     /translation="MPEGIHYDNWMDAFMEVKYPVSTEDHTNTQLWFWVDYDTEAGRD
                     FLQVQSQFGDLVWYAISPPYSGSPATERLLPPPPNRPGSGGATRHRELPDATPLVR"
     gene            6181..7329
                     /locus_tag="PKM94_03515"
     CDS             6181..7329
                     /locus_tag="PKM94_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65737.1"
                     /translation="MKYRSGFLILFGLGCMGLVASPAEAWKENYVGYFDCSTKVVNAN
                     PATGQVTVAVTLTPLSGYDRRCEAVSFEITDIEDVEILSPLRWDLPLIDSITPLVAMI
                     DLRVPTHDTAGFALVTTCGTWNYKWPQFFGPAGDSIRWFWSDPRTWPPLGAPAPDSVR
                     ARLAQERSERYLEWLRQQPPPPPPQGYSSTYIIIYPGDSALADSLTEQGKSQLAGMRK
                     REQSPLVDADCEPWSVDDRHFIRHRGESRFRPVETFTLDQARERAQRKRDSLEVAQAH
                     ITYEVLVDLRNPADSLWVAAHVDSLIPTDSTGFYRIKINKRTWRQMGEAGVPWWETST
                     RKPPVPRSHPEPPKPPRSERDATLPLHLADGRSLLFFEGFEGAWPGAC"
     gene            7368..8456
                     /locus_tag="PKM94_03520"
     CDS             7368..8456
                     /locus_tag="PKM94_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65738.1"
                     /translation="MRRWTRACGIMSAGSILAGLMLIVGLLGPACDDDPTGPDHPDLP
                     DGKDYVFYYRDQATGTLYGYHLVSERVDTFTVPYVARFGMFVSADGQRLYVAGPQATG
                     VVALATRTLVAELPYAQYPVGNGIIFSPDGRFMAFLGSTLTILTYPDHEPVFRDTMGA
                     GTGCFTPGGERLYYVPPGADQVRVVETTGGEARVHPRVFNPRLSKIRVLSDESRWVLY
                     QSISCDIGGVVSVYDPIRDTMEFQEWFYLSCGMLAVSADDRWAFYDNPGPLLNPVPGT
                     PLEFRVLNLVTKRLDRLVSTVGIADGLNPQDMAVGWLYVSPDSRWLVAAGTWGDQFLV
                     FDVRHLKVDRYIKLDGFWSDMFAGQEGL"
     gene            9241..9438
                     /locus_tag="PKM94_03525"
     CDS             9241..9438
                     /locus_tag="PKM94_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65739.1"
                     /translation="MKETAKYFLWGVALSALLFLIDRLTGIDDLPYGWSLIAGIGVAI
                     LVGAVFAVVYRLIRRRRAKNG"
     gene            9546..9788
                     /locus_tag="PKM94_03530"
     CDS             9546..9788
                     /locus_tag="PKM94_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65740.1"
                     /translation="MISLVALGLMVIGIVLLVLAYRRQRPDAPRLGSVDAHQPRTWRP
                     VWRQRHLFAEKRGFVCYLIGATLIVSGGMLGAVFVF"
     gene            complement(9847..10692)
                     /locus_tag="PKM94_03535"
     CDS             complement(9847..10692)
                     /locus_tag="PKM94_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD65741.1"
                     /translation="MSTETTSIRKAHRLVHGEGATILCLHASTSSSKQWQPLAERMAA
                     RYRVVAADLYGYGQSPEWHGERPLTLDDEISLIADEIAAADEPVHLVGHSYGGAVAAR
                     VAVAYPDRVASLTLYEPVMFGLLRDDPNSRAAWEEIRAVERFVRTRTAAGRREAAAQH
                     FVDYWSGAGAWTSIPEWRRAGLAAKMGKVTADFQAVCGMPGDLVPFREFPRPTLLLCG
                     LRSPATARRVIQLLLAAMPETELVRIAELGHMGPVTHAGEVNARIEEFIDRVERRRSQ
                     RPQVA"
     gene            10983..11954
                     /locus_tag="PKM94_03540"
     CDS             10983..11954
                     /locus_tag="PKM94_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65742.1"
                     /translation="MKYLSRMILLLPAAFVCLAGRPAAQDSLKIYLELGGKAIQNSKY
                     DRAIEVYTRVLQLDSANYEAISNLGVAYASLGNYARARGCLEQALRRNPGDAQAANNL
                     GTVFSNENDRTAAIEYFEKAVGLAPKNALFLTNLGREYARDGQVGKALPVLREALVLA
                     PDNPLIPYSLGSAFASAGSLDSAEVYFERSLAKGGTGVELHYFLGTVKDRLGKKDEAV
                     NHYKSALEMEPDHLNCLQSLGLIYLSQDRFPEAIEQFRRVIAVDSTQWAGWIGLGASY
                     SLYRMIPQADSILGRLFQADSAIGFQMLDFIGQQHRLRRDSTGSKTR"
     gene            12161..12706
                     /locus_tag="PKM94_03545"
     CDS             12161..12706
                     /locus_tag="PKM94_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65743.1"
                     /translation="MHARVRALCLAGLVLLCSPAAAQDWFNGGPDTLAGADPAPRPET
                     ISDFMHAADLWHDATLKGDPRMIRKRELQLVRFLNEDIAASRRRYESCAARVEAAGGP
                     DSGRVGVFPPSAQRALDDLARAEQWLRVKKRLLRGFEQGGTFAAQYRQIMDYRDILRM
                     EYDQWRVLVADGASADSAETK"
     gene            complement(12785..13633)
                     /gene="arsM"
                     /locus_tag="PKM94_03550"
     CDS             complement(12785..13633)
                     /gene="arsM"
                     /locus_tag="PKM94_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOD65744.1"
                     /translation="MTDKRSPEEIRKAVREHYGTAITKGTGGCCGPQPQALDPAAARR
                     YEELAGYRAGETEGLPDGVTSFGCGNPVNFAEIKPGQTVLDLGSGAGLDLILAAKRVG
                     PAGRVIGLDMTPEMIATCRRNLAAAGIANAEVVPGEMEHMPLPDASVDWIISNCVINL
                     SPDKEKVFAEAFRVLRPGGRVLIADIVTNSLPEEYRDDLSAWVGCLAGAPEEDEYLQL
                     IRAAGFEDVRVIDKMTYDEASLGTLANDACGCGASDRAIDRGVIARFAGKVASARISA
                     RKPERG"
     regulatory      13763..13860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13945..16425
                     /locus_tag="PKM94_03555"
     CDS             13945..16425
                     /locus_tag="PKM94_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD65745.1"
                     /translation="MRCAVLITIMLAPFAAAVEAAPLSGMVQNPDGEPLAAVSLTTNV
                     AGLGTSTDAAGAFTLECPGDVRWVTFSSVGYQPRQYPVDAVPARVVLAYEYSPGGDVV
                     VRGDRAQPGLSPIAFTDYSRKDIARDYSIGEFPLLLQATPNLYTYTDGGGALGYSSVR
                     IRGFDDKRIAASLNGVPLNDPEDQAAYFVDLPDFAANITDIQVQRGVGNALYGAASFG
                     GSLNIVTGALSRDRRVTVTSGYGEYTSGGDRVSDLAKSSIEYVSGLIDGRWLFTGRFS
                     DQRSGGYRRHSWYDGWSYYLSLARLDPRMTTELYLYGGPIRMHLAYYGVDRATLAVDR
                     RANPLTYRNETDNFNQPHFHLHNTYQLSERATLATTLYYIQGEGYYEQLKQLEDPDDF
                     AVYGLDPGELAVDQPRLVRQKWVDKRQWGWNPRLDLAHARGRHSIGGSAYYFTSDHWG
                     QVVWVEGLEDRSYGPRQRYYRYEGRKVEANLFAQEYCRLTDRLAGEATAMLRYQRYDF
                     DQTRMYSFRGFRYDVDWLFFSPRLGFTYTLAPEIALHTAFAVSSRTPTDEDIYEADDP
                     FALPSLDIRSVSADSARWEFGDPTAKAERVYDFELGGGYRSDRYAVELALYWMEFRDE
                     IIPEGAVDASGRRRTINADRSVHAGLELSGTARPTEALALSGNFAWAYNRIRDYTASL
                     DLYDTDWNYAGQYRFDLADKTVPGFPAYLGNVLLDYRYRGLRAVWRHQFVGAQELDLA
                     NTDSLRIEPFQLASVSLSYRFSDFWHLGALTLTARVNNVFDRRYEAGGYGWNYAVAGD
                     GGTDVLGGAEYFAAPERWYYGEVKLEIF"
     gene            16442..17842
                     /locus_tag="PKM94_03560"
     CDS             16442..17842
                     /locus_tag="PKM94_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOD65746.1"
                     /translation="MHLVDYAIIVAYLAVLLYLGLRNPLTGKNSTGELILGGRLLALP
                     AFVASLVSTWYGGILGVGEYSYRHGLSNWLVFGLPYYLAAALFAVLLARRARESLVLT
                     IPDRLAQAYDNRTAIAGAAIIYLMTVPSAYILMTGVLCEYLFGWPFWLGVVVGTFFSI
                     VYVYTGGFVSMVRTDMLQFVLMFLGFIVMLGAAVARYGGWSFLRSAVPADHFTWHGGN
                     APFYVAIWYVIALATLIEPAFYQRCYAATSVRVARVGIYISIAFWAVFDFLTTVTGMY
                     ARAILPELADPVSSYPALAFHLLPPGLLGIFALGLLATVMSTVDSYAHIAATTFGNDI
                     VGRAARLKAGGILFYSRLGLVISSVLAILVAIFFRSVVDIWHAFGSVGTPALLVPVVT
                     SFVGRRRLRPAWALLSIVACGGLSALWLAASYVRADGGYWFGIQPILPGLVLSLLLFA
                     LFSRPVAPGNLPTPPD"
     gene            17858..18334
                     /gene="greA"
                     /locus_tag="PKM94_03565"
     CDS             17858..18334
                     /gene="greA"
                     /locus_tag="PKM94_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOD65747.1"
                     /translation="MSEPIYMSKDGVRKLEVELTRLKFEERPKIVADIKRAREHGDLS
                     ENAEYHAAKEAQAHLERRIAELELKLSRVQTIDTDAIPSDRVYLYAKVLVEDMVDGEQ
                     IQYAVVPPAEADVDNDIISVESPIGKGLLGKAEGEVAEIAVPAGMLRYKILKISRD"
     gene            complement(18533..18799)
                     /locus_tag="PKM94_03570"
     CDS             complement(18533..18799)
                     /locus_tag="PKM94_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65748.1"
                     /translation="MAEDVEKPDRGAGHRTRAQKWQEGHNLRDTSMQCAQCGEEIKGK
                     PVKQEGEIYCSLECAYLAAGLEPEEDEDYFEEDPVEGTFGDDDE"
     gene            19283..20527
                     /locus_tag="PKM94_03575"
     CDS             19283..20527
                     /locus_tag="PKM94_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOD65749.1"
                     /translation="MSIVRPFKGLRPRPEYAGQVAAPPYDVLSSDEARAMAAGNPISF
                     LHVNKPEIDFPPDQDPTAPAVYEAGRKNLQALVDRGVMCRDTSPCLYLYRLTWRGRSQ
                     TGLWALTSCDEYNRNLIKKHEHTRPAKVSDRADHMETLGAQVGPVFSCFRHNEEIRRL
                     FDHVKKVPPMIDITTEQGVRHELWVISHRDTLDSLAAAFGRLPYLYIADGHHRSAAAA
                     EVAGRRAAKNPHHSGDEHYNFFMNVIFPDDELRILPYNRVVRSLNGMTLEELFARAGD
                     TFTFAPSDAPVEPQKPYEFGLYAAGRWYRMSARPGKVAAAGPAEAIDSAVLTRHFLTP
                     YLGIKDIRTDDRIDFVGGIRGVNELERLVDSGAFAIAFSLFPATVDQLLAVADAGEVM
                     PPKSTWFEPKLRSGLVVNYLDE"
     gene            20545..21423
                     /locus_tag="PKM94_03580"
     CDS             20545..21423
                     /locus_tag="PKM94_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOD65750.1"
                     /translation="MLILISDAFDASLPGILSRFGEVTDDKSRLADAEIVLIRSKTKV
                     TREYIDSAPRLRYVIRGGVGLDNVDVAYARTKGIKVDNTADASTTAVAELAFALMIAL
                     PNHIAAADASMRQGKWLKNELKRTELYGKTLGILGIGRIGLSLAVRARAFQMRVLGWH
                     PDVYFTDFAEICPTMEEVLERSDYVSLHMPLVDSTRGIINKETLKRFKDGAYLINTGR
                     GKCIVEQDVVDALKSGKLRGYATDVWNSDPPENSPLLGAPNTILTPHIGASTKENMIR
                     IGIIVERLIEDYTAKK"
     gene            21451..22533
                     /gene="serC"
                     /locus_tag="PKM94_03585"
     CDS             21451..22533
                     /gene="serC"
                     /locus_tag="PKM94_03585"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOD65751.1"
                     /translation="MAHRVYNFNPGPAVLPLEVLETVQKELLDYKGTGMSILESSHRA
                     AEYTEVNDQCIALARELFGLPADYHVLFMTGGASTQFALVPMNFLNGKTGAYIDTGSW
                     SNKAIKEAKIVGKAHVCYSGKEGGYKHIPTQKELDIPADAAYLHMTTNNTIYGTQFSS
                     IPDSKGLPLIADMSSDIASRRLDFTKFDLIYAGAQKNIGPAGVTLVLLRDRLLKTANP
                     NLPTMFKYGTHAEEKSLYNTPPAFGVYVMKLVFEWIKRQGGLAAVEKVNLAKKERVYQ
                     IMDLYPDYYRGHCDKESRSWMNITLRLPTEDLEKKFLAEAKAQGFIGLKGHRSVGGIR
                     VSAYNALPLEGVDKLVAFMEKFKKQN"
     gene            22994..24133
                     /locus_tag="PKM94_03590"
     CDS             22994..24133
                     /locus_tag="PKM94_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOD65752.1"
                     /translation="MGIYPYFHPLQSAPGNEVIVDGKKCIMVGSNNYLGLVDHPKVKE
                     AAAEAARKFGSGCTGSRFLNGTLDLHLELEERLARFMQRPAALVFSTGFQTNLGTIAT
                     LVGKNDAVVIDRSDHASIVDGTRLSFGKVYKFAHNNTEDLDRVLARVRREITRGGVII
                     VVDGVFSMEGDIINLADLVPVAEKYNARVLVDDAHAVGVLGKTGAGTAEHFGLIDRVG
                     ITMGTFSKSFASLGGFIAADWEVINYIKHRARALIFSASIPPSNAAAVLAALDIIQAE
                     PERRERLWKNALRMQKEFRDLGFNICHSQTPIVPLVVGDDLDAFAFWKALFDNGVFTN
                     PVISPAVPPGQAMIRTSYTATHTDDQLDRVVEVIAKVGREKGIIP"
     gene            24140..25270
                     /locus_tag="PKM94_03595"
     CDS             24140..25270
                     /locus_tag="PKM94_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOD65753.1"
                     /translation="MPNNVQVVEVESRAQLRDFITYPNRLYRDDPCYVVPLLSERLEF
                     FDFEKNPFYRAARVRLFLAKRGEVTVGRIATCINYAHNEFHGEKTGFFGFFDCPDDYD
                     VAFVLLKVAMIRLKMEGMEKMRGPMNFSTNHEIGFLVEGFDEPPRIMMPYNPPYLPEL
                     AARFGLRKAMDLLAYKLTDETPIPERVETVIGKIRQRGNLVVRPIRLRDFDNEVDRVN
                     QVYNQAWQNNWGFVPMGDAEFRWIARNLRQVIDPDLALLCEHEGRPVAFILGIPDINQ
                     ALARLRGRLFPLGLLRLLWHTKIRNKIDGVRVLTMGVIPPYQRRGIDMMLYTEVYRRG
                     VKKGYRWAELSWILETNTLMRSAADAIGAKVYKRYRIVELPL"
     gene            25347..25916
                     /locus_tag="PKM94_03600"
     CDS             25347..25916
                     /locus_tag="PKM94_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65754.1"
                     /translation="MPNVLPRNTRAVLGAVLLAALLGAACTSRYRMELYYLADAERSR
                     IDVEQALFTPATRLNDPLAVPDIVSGEGSTAVITVGKRGERYPGTGAYRALSFDQYLQ
                     YRIYLELPSPPWDGLPDTIPVVGRSYVRLMRFYERPPAERTYGPETGAFVIDSVRGSS
                     LYGTFDGAQWVNGAGHRVGFEGRVKMRVK"
     gene            complement(25938..26459)
                     /locus_tag="PKM94_03605"
     CDS             complement(25938..26459)
                     /locus_tag="PKM94_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOD65755.1"
                     /translation="MTENQAFTVEIVKGNIVEALAEAIVNAANTKLWMGAGVAGAIKA
                     AGGESVEQEAIAKGPIPPGQAVATTAGRLRYKYVIHAAVMGQDLRTNDRYIRQATIAS
                     LNLADRLQLKSVALPAFGTGVGGFPMTACANIMVKAARGFRTEARHLERVVFCLYDEL
                     GYRAFLKAAEAHR"
     gene            complement(26459..27070)
                     /locus_tag="PKM94_03610"
     CDS             complement(26459..27070)
                     /locus_tag="PKM94_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD65756.1"
                     /translation="MLETLNAVDRALFLFVNMTLANPVTDLLMPVVTSDVLLRVGYGA
                     AMVILLLRGDARVRRVVLFSAAALVLTDQLSSAVLKPLIARPRPCQALTDIHLLVACG
                     GGKAMPSSHAANAFGQAALFALAAPRWRWLLLALAAVIALSRVFVGVHYVGDVAVGTL
                     LGAAIGTAAAQGYRWVETRMAERRARPGCIPPNESADGKKARE"
     gene            complement(27063..27986)
                     /locus_tag="PKM94_03615"
     CDS             complement(27063..27986)
                     /locus_tag="PKM94_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD65757.1"
                     /translation="MRQTTTTPLDALGMGIIPLDYLAAIPRFPELGGKADATHMTVQG
                     GGPVPNAMVGLARMGCRTAVIAAIGDDHPGQVLLAELAREGVETAHMIVKKRTPSATA
                     YGFIEPDGRRTIVLHRRIFIEPRDLRLSHYPVPRVLHLDGRDIEATVRLARWGKRAGA
                     TVTFDIGSMRNDVTPVLPLVDHLVVADSWALPYTGERETPRALARLAEICGGTVVITE
                     GIRGATGLEAGRRSHHPAYRIEAVDTTGAGDAFHAGYLYGLLRGKDMAERLRLGSAAA
                     ALKCTRPGARGGMPTLSQLRKFLQRPPETYA"
     gene            complement(27958..28581)
                     /locus_tag="PKM94_03620"
     CDS             complement(27958..28581)
                     /locus_tag="PKM94_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOD65758.1"
                     /translation="MEDVYHTIARPARHEIKVKASRFIGECAEAGTPSAALEFLAGIR
                     RREHAATHHCFAYTVGLSEGAMFKYSDDGEPSGTAGRPIYDAVIGRGLANTIVVVTRY
                     FGGTKLGPGGLARAYSETAAGALDAAGTRANYLTERLEVGIEFPLYDLVAKAMHQHEA
                     QQVEAEFTDRVRLVIAVRRSRAVALRRDIVQISGGKATIAADDDDTA"
     gene            complement(28587..29495)
                     /locus_tag="PKM94_03625"
     CDS             complement(28587..29495)
                     /locus_tag="PKM94_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD65759.1"
                     /translation="MEEHIPGYNQAVRVLALCRYAGVGPRLFDLLMGRFGSLDAIFAV
                     SASSLHAIDDLEDDRAEQIRQAETHLGEAQAEYDRLRERDIRIVTRLDAEYPRRFEEL
                     NDPPPLLYVRGSLPANDRKAVTLAGASDATAEGIELTVDLARRFAADGVQVVASLRRG
                     IDAAAHLGARAAEQGSFAVIESGLDRIDAAEQLPVAIDALRGGGVLSELAPDQGHEQG
                     GFAAANRLLAGLSNAVVVTEIYADSREALDLVEFCSQIGKLVFIVAHHRLGPLSDSAA
                     MDRLTEYGAVPLDSPEQADQIIAALV"
     gene            complement(29504..31081)
                     /locus_tag="PKM94_03630"
     CDS             complement(29504..31081)
                     /locus_tag="PKM94_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65760.1"
                     /translation="MVPRPALFLSVLLASSPVQAVDSSHPKVQAPVAVVAPMDTESTA
                     DQPAALADRLGRSLANHLAHLAAMTGRGVTVTLRETPAEDRGLLDSLAGEGVREVLVV
                     RWRGRAGERTGEIRDAHRRRYKAVVEDCLQIEAAYERRQLTAHGWEVAAGGTAAAAGR
                     PDPNARRRPGRTLAGEPYERVVQRLVEQIGDELPPAEPLARALRATAPVKVYLGKELA
                     ADSGAAMALIRRIVDLASTHLAAQFGVALRIVSAAPLPENAADEGSLAAGYPEFRRAV
                     SVSADTLAVAFHRMLSREQYFGGGVREEIGMAQLGRRRVYMDLLPAAAPGDTLWQAMA
                     NALTLLHEIGHALGGIHVSDVNSVMAHGANWVNAAAFDRVNEKIIGAALTGRLRFDDP
                     AEYLATVARVLDSTAYGVADYPGFFHQFLSGIDHPADRKRLREAVGRTSFLQAADAVG
                     LLAAGRRAAAARLLRTALHEDPDQAALYYYLSLAETGPAADGARKMAERMGYLMAGER
                     SRTGAPDAAVDIARTRP"
     gene            31165..32013
                     /locus_tag="PKM94_03635"
     CDS             31165..32013
                     /locus_tag="PKM94_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65761.1"
                     /translation="MLRLLPIVLAALLIAGCGGEPKTEAELREAGKKAFVKEDFAKAR
                     DYLGRLVRLHPSDRDALYFLGMAYSKDLKYDSALYYLERVNTLYEDDREANLELYRIG
                     MLTQDAKVAKDAIGALIRTGDPADMYYEDLARLSLMLKQYALATRYFRILLEREPDQP
                     QRYLDAANAVAGLDSLAVAVALLDSAAARFGARPEFLLNKSLYLTAQEKYGESEAIIR
                     GLIAADTGNLGYKVNLAHVLSSTDSEAKRREAYALYHDLKGRVDARFAVDSLMADLAR
                     KLDSAQ"
     gene            complement(32088..33014)
                     /gene="secF"
                     /locus_tag="PKM94_03640"
     CDS             complement(32088..33014)
                     /gene="secF"
                     /locus_tag="PKM94_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOD65762.1"
                     /translation="MFTIVPETNINFIGARKIAFAISIVLVIGGLFATVLVWTGKADL
                     GIDFAGGTMIYAAFEKPVAIEDLRMAVSSAAPDAQITQLQNFEKPNAFILKTKRPTVE
                     GEGQQRLTAIEGAIRQRFTDNSFEKVSEHIIGPAVGESLRSDTQKAVLLSLLGILVYI
                     WIRFDFRFGVAAAVATFHDVLAVLGIMLLLGFEFDLLLVTALLTLAGYSLTDTVVVFD
                     RIRENLKKWRTKGEFAGAVNRSINEIISRTINTGGTTLVSILALTIFGGEVLRTFSVA
                     MVLGIIVGTYSSWFVASPIVVEWEARRPKRFK"
     gene            complement(33034..34758)
                     /gene="secD"
                     /locus_tag="PKM94_03645"
     CDS             complement(33034..34758)
                     /gene="secD"
                     /locus_tag="PKM94_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOD65763.1"
                     /translation="MSKNNWRIGLTLVLVVAALFAFWHTFSLWTMSDAEKARMQEEQP
                     GELLKLQQKAIRLGLDLQGGIHVVLRVDMAELAPAERDGAVDRAIAIIRNRVDGLGVA
                     EPTIQKQGTDRIIVDLPGYTDANRAEELIGQTALLEFKLLETMDNAQLLLTKIDSAVF
                     GYEKAKAGAAPETPGEPAPTAPTADSAAQEPDVMAELMGDSATKDTTEDPFAFDENTA
                     DAENEKPLSSRLSPALYSSQSGEGWPGFYVAKDQKERIDRWIGLPEVQALMPIDVQWA
                     WSTRSEIRESREVYTLYLLKRKVQFLGRFLENIRLGQGQYGEYTVDFSLSGDGSARFA
                     QLTGANIGKPLAIVLDNRVESAPIINSKIRTNGQITMGGGASIKDAQNLEIVLKAGAL
                     PAPVNIIEKNVVGATLGADSIRKGFYSALLGLCLVLLYMAVYYRISGLIADIGLLFNI
                     FFLLAVMAGLSATLTMPGIAGIILTMGIAVDSNILIFERIREELRTGKTVRAAIEAGY
                     DRAFVAIFDSHVTTLITAGALFLLGSGSIKGFAVTLFWGVAISLYTAYVVTKQIFDIR
                     KSYRSLSI"
     gene            complement(34846..35280)
                     /locus_tag="PKM94_03650"
     CDS             complement(34846..35280)
                     /locus_tag="PKM94_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65764.1"
                     /translation="MTKRDRIELLRRSARTGLTGLVFLIIGGTGLFVAGITWSAGTDW
                     EGPGDGYACTYPPAGHGGQQSMTPNGQPPGEPSDHLILPLIIKTDNSDKRPAAADRAY
                     FHTCRRAGEVRDRTSPCTSTAAVHPLLGRQFTLVGAKPSGTG"
     gene            35437..38208
                     /locus_tag="PKM94_03655"
     CDS             35437..38208
                     /locus_tag="PKM94_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD65765.1"
                     /translation="MSKPVPVIAVLLGALSALVAGPAGSCRAAETYELDNGMTVILKE
                     QHGAPMVSSIVFVRSGSKYESRFENGITHFLEHLLFDGTATLAREQLDASIGDLGGYI
                     NAFTRKELTAYLVLLPKQYIEYGMTVQADMLFNSVFPEAELAKERKVVVEEIRRDADA
                     PGAAAEAFFTDRAYAGTDYARPVLGYPAFIENIPRAAVIDYWRRYYIPRRMTLLVIGD
                     FEPAAMKKTVANVFGRFTNPASAADSAASASLAPAPVLEGQTIYDTAAQVPSTYLNLS
                     LAAPPIGSAEYYAMDLLARYLAMDAVSPLMVALKGGAEPLATEASVSLVPYEEFSRLE
                     ISVITEHPERRDSIITVIFANLAAMARHAADPGSIAGLQTSVQTDDIYSEAKLHYYGF
                     MIAPYLMTAGWDFVQTYAEKMAAVTWADCNAAADRWLARPAYIGTVVRPATDSAAAPY
                     RPEEMSADEVLAHFAEATFPEHELVAGHTITYPPTDSISFTLTDRASYRREQLPNGMT
                     VIVKTVPDNEVFALMVLGRNRSANEPPGRAGITEFVNRCLERGTATRSAKELADALAG
                     IGAQVTLYDNPWIPYDDRYTARAFSFLKFETIEAFARKGFALFSEVAFSPAFDSAEVE
                     NVRQAMIGVLRRQGTSPREAARDLFYTTLFEGSAYARPIMGDFESLNAITVDDLRAHH
                     RRIYAPENTIIAVVTSRPADEILGWFAEGPGRFAPTGFVSAVPAPPQPVRQIIAAQVP
                     LEKEQVAIYLGGPTPGAESPDALALEMAAGILSNRLYLNLREKQGLAYSVGAGADADR
                     GFGWCYASMQTGVDNYRQAVGGILLEMDKLRYDGPLASEVRRARNEIWGRLMSAKLSA
                     INQAFYLAVDEFLGRPLPYDRTLLDRLGAIDREGVRRVAAQYFRTDAYVIATAGRTPA
                     ETAPPAPGE"
     gene            38222..38902
                     /locus_tag="PKM94_03660"
     CDS             38222..38902
                     /locus_tag="PKM94_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOD65766.1"
                     /translation="MFRRLRELFGSEDLLQAAFATTLTMLEMDLRMFEASSAALRRSD
                     SAELPFDVRQEDRRINKYEREVRRNVLTHLSVAGTQNIVPGLVLVSIVIDVERIGDYT
                     KNIAELAQLHPGRLEGGPYEGEVAEIESQVNRIFPLVLRVLREQEAELARNIMAREDR
                     TGKMAEAIVQSLISEKGRGLPVAEAVSLALYARYLKRINAHLTNIASAVGNPFPRLGF
                     RAKPDQGD"
     gene            complement(38961..40199)
                     /locus_tag="PKM94_03665"
     CDS             complement(38961..40199)
                     /locus_tag="PKM94_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOD65767.1"
                     /translation="MADRPHRYDIDPYLWLILIGTFLSCLPGLYLVAAGKHLYAPYAA
                     VVFGVAIFGAAFILSWAAEVAQMDMSQSLAVAILALVAVLPEYAVDFVFTWKAAHDPT
                     QAHYALANMTGANRLLVGLGWPAVLFLYILAKRRREVRLDETQRVEIFYLAMATLYSF
                     TIPLKGSLNLIDTVVLVTLFGLYTRRAAQMPQEEPEMVGPVKIVANAATVPRRLFTAL
                     FFLFSGFVIFLVAEPFAHALVESGGHLGIDEFFLVQWLAPIASESPEFIVAATWALRG
                     QAGAALKALISSKVNQWTLLVGTIPLVYAISAGEIRPFVLDVPYGEGPTDVQTHELIL
                     TAAQSLFAVAVLVNLVLTRWDGVLLFGLFALQLVWQDIRMEVAAVYIVLAVIWHWVNR
                     RHLLPAARIGLGIRGRPGTS"
     gene            complement(40227..41132)
                     /locus_tag="PKM94_03670"
     CDS             complement(40227..41132)
                     /locus_tag="PKM94_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD65768.1"
                     /translation="MKLANLLMDRRIRIDLKAHTKAEAVDELLDLLRAERVALDYDDI
                     VRSVRIREEIESTSYGRGFAFPHARTNAVSELYILIGVSREGLDEGGPDGVPVHVVCL
                     LLTPSAIAKLYLQTLSGLATFARMPGSLDRVRSIINPRDLVKLVADTGVTVDKELMVK
                     DIMRHDVAKVRPDDTLKDVANEMFRHRLSALAVVDENNRLLGVISDRDLIKAALPDYR
                     TLISNLNYSLDVEPFEELLKREDKIKVSQLYREDFETTTPETRIVEVAAMMIFKDFTR
                     VFVTENDNLVGILLRKDIVNMIIRG"
BASE COUNT         6996 a        13955 c        12973 g         7298 t
ORIGIN      
        1 tgtgcacttt gcccacgtct ttgagcaccc gggccgtgat ccccaccgag gtcgcggtca
       61 gcgtcgcccc tatgaaaatg tggacataga tcgactcctc cggaatgaac cacgccgcca
      121 ctccccagcc gaggaaaaac ggcgcgatca ccccgaacgt cgccaccaaa aacgacgcca
      181 ctcccacctg catcatctct cgcacggtcg actccagccc cacctcgaac aggagcacca
      241 tcaccccgat ttcggcgagc acctggaaaa tcatgttgag gttcgggtcg tgcaggtagg
      301 gttgaaaggc gacgatcccg atcaggtggg cgttgccgac cagcatcccg agcaccagct
      361 cgccgagcac cgccggcgcc ttgatccgct cgaacaggtc cccgcccagc ttggccgcca
      421 gaatgatgat gatcagttcc aaaagcaccg agaggacttc gctcgcatgc ccgcttccgg
      481 attgctcccc gtgcgcgacc ggtaccccgg cggccggccc cggcgcggcc ggctgctcgg
      541 cgccggttgc cgcctgggcc gccgggggat tctggtcccc ttgcgccccg gcggcgctgc
      601 caaacgccag gctgtacacg atataggcgc cgataatcgc cagggtcgcg aagaaacgtc
      661 gagtcgtgag cagtcgcacg cacacctccg gagtcgttgg cggcgcgccg ggtggtgatc
      721 ccgaggcgct gcggggcagt caattcggct gaaactagcc aaaagccgga gggcgaacaa
      781 gccttttttg gcccggcccg cccggcgccg gagcccgcgc tgcgctcgcg cccgtagcgg
      841 ccgttctgcg cttactcagg ggcaaaacta tcttttgtgc ttgacagccg ctgtcatctc
      901 atcgttatta gggttgggta caggctggtc aggatcacac accggtttag ccggacacaa
      961 gagttctcag aagtttggac ataacaggcg tgcggcttgg tgttgccgcg tgattcacgc
     1021 ggcggcccgg gttgccgtcg ctatcgataa tgcacggttg atcggcagac cggcaagtag
     1081 gcgtcggtcg cggttggtcg aaccaaggga gagaacgcat tcgtttgacc gaatgcaaaa
     1141 gcaaggtgtt gctcatgaaa cggttgatta tcggaggagt gattgcggcg atgagcttgg
     1201 cgttgggctc gagcgccgcg tgggcaagtg cgtgggaccc ggagcatccg ccggcgtgga
     1261 taccggtgca aatggcatcc gagcgcccca gcggtgacga aggtagctgg gttgacccgc
     1321 ataatcgcat cgcaagtata tcgcccgtcg gagatctgtt tcagcgattg ctgctgaaag
     1381 tcactgtgtg gttcattgta ccaagtgcgc aaccgtcatc agagacaacc attccagata
     1441 ttgggcaaag taccggggga gtctcccatg tttcaggaag ctgaagcgct cctgagccag
     1501 aatcggttca ccgaggtaat cgcgctgctc gatcgagttc caccccaatc tctctcgacc
     1561 gaggaacgcg gttactgctg catcttgaag agcgaggcca tgctctatct cggattggat
     1621 ccgggcgacc tcatcgatca ggcgtccgag gtgttccgca tgcatgccga ggccgagaag
     1681 ttcgcacgcg cgaaactgct caagggctgg tatctgacca ccatcggacg ctatgccgat
     1741 tcccgcgagg ctcttttgga ggcatatgcc aattatctcc ggtgtcggca caagcgggga
     1801 gcagccaggg ccctgaaccg ccttgctttc gtgcaccgcc agctgggcca ctaccgagct
     1861 gccgaggaga acctgactcg cagtgctgaa atctatcgcg aactcgccga tgaaatcagc
     1921 atgaccggag tgctgaccaa cagagcctac ttgttgatga gcggcgggag tctccgttgc
     1981 gctgagaccg agtactcgaa gatgcgtgac acggtcgtcc aagccggaga aaagaacgtt
     2041 gtagcccacg gcaacatgtc ggcgatgtgt tacgccctct tgggcgatct caccaccgcc
     2101 cgaagaacca tcgacgaggg caaaccctac ctcgacacct acatccgcga gaaagccatc
     2161 taccacgaga acctcggcct catcgaaatt ctctccggga attacgaggc ggccgagcag
     2221 tcgctgcaaa ccggtctgga catctcgctg caaatcgccc cggaatccgc gctggtctcg
     2281 cagatcaagc gcctcttcgc cgacctctat attcaaacgc agcactggac gaaagcagag
     2341 aagtttgccc gggaagcgct cgctgtggcc gagcagatca acgagcgcct cgaaatcgcc
     2401 gcctgctggc gggttttcgc ccaagtcgag aaccaccggg gtaatcatga ccaggcccgc
     2461 gcctggttcg accaagccat cgacctcttc aaccagatcg gctcccgcta cgagctcgcg
     2521 gttacgcgct gtctcgccgc cctctcgaac ctgtacgacc ggaacaagcg ggtcgcgctg
     2581 ctctacctcg cgcgggagta ttttgagtct gaaggggtgg ctccctggct gaccagggtg
     2641 acggaggctc tggagaacga ggcgccgccg gcgccgcggg cccggccggc cgcagcggcg
     2701 gccgtcattg tcggggcgga caagtcgctc aggcagattc tcgcgctcgc cgaacacgcc
     2761 gcgccgaccg acttcaccgt cttcctcacc ggcgagacgg gcacgggcaa ggacctcctc
     2821 gcccggcaca tccacgcctg ttccggccgg aagggggagt ttgtcgcggt caacgccgcc
     2881 gcgattccca ccgacatgat cgaggccgag ttgttcggcc acgtgatggg ggcgttcacc
     2941 ggcgcagccg ccgaccggcg gggtctcttc gagcaggccg cccacggcac attctacctc
     3001 gatgagatcg ccgatgcgtc cccgcttttc caggccaaac tgctgcaggt gctggaatcg
     3061 aggcagattc ggccgctcgg ccagaacgga ctccgtccgc tcacctgccg gtttgtcgcg
     3121 gccaccaacc acgatctgca cgacctgctc aagagcaatg cgttccggcc cgatctgttt
     3181 caccgcctca accacgtgcc gatccacctt ccgccgctgc gcgaacggct cagtgacatt
     3241 ccgctgctgg tggagcattt cctcaaggag gacggcattg tggcgcggtc tcggaacgaa
     3301 acgcttgcgc ttgagcgcct ctgccgcctg ctggccgccc ggtcgtggcc cggcaacgtc
     3361 cgccagctcc gctcggtcgt gctgcgactg gcgatgatcg gggggcgcga tctgccccgc
     3421 atggtcgagg cggcgcgcgc cgacatcgac cagccctgcg aggcgcagga tgaactcctg
     3481 tacatgctgc aaaccaccgg ctggaatcgg cgggagaccg cccgccggtt gggggtgagc
     3541 gaagcgactg tccgccgccg catcgccgaa ttcgacctcg cggtgcagat tcctcagccg
     3601 ccggcctgat cctgtagatt cggctgtccc cggcctgcgt catgagcgcg gttcctgacg
     3661 cactcgtgac gcaggcctcg ctttattaat gcctttagtt tcaacagctt tgcgcggcct
     3721 gcgtcacgct gcgtcacgcc gtgcgtcaca acgacgtccc ggccccggcg cggctgctcg
     3781 cgcacgccct ccgcattcct cgccaactgc ttccccgtca atgcgatgct cagaaagctt
     3841 tcaggcgtct ctctgttcgg cacgcgggtt gattatagat cgcatgcaga cggttgggtt
     3901 gcggcggcgg tgacacgagg catctgccta tcgccgaccg caagctaggg aagtcgcagg
     3961 catggtcctc acacgggtcg ggccggctgt tcccgccgag cctactcaac gcaccgggaa
     4021 caccggcctt ccccggggga ccggacacgt ggtgaccgat cagctccggc gacatgtcag
     4081 agcggatcgc gatgcacttg aagcggcatg cgacagggca gtagcacgaa gagcggcttg
     4141 acggaccgcc ggctgggcga cggaatgagc agcacaatgt catggagaat agcggggatg
     4201 gcagtgcccc gagggcgatc tgagagtgtt cgcccatgcc tcttgttcct cttgtgtcca
     4261 atgcacttcc tcctggacgg cggcggggag ttgacggtta ctccccgctg ccgccaatgc
     4321 cgcggagagc gcgtgccgcc ccggacgccg gtctgaccgg ggacactgga cgggaagaag
     4381 ccaatggacg tcctgtagat atagcagtat cttacgacca cacagcacag gagggtacta
     4441 tgaggtatcg aagcagactc gctctcttcg ggcttgggtg catggcgctg ttatgggcga
     4501 cgcttgcctt ggcgacgcca ccgggggttt ttttgccctt tgcactcgat ctccgcattg
     4561 tctcctatga ccagtcgacc ggccgcgtcg tcctggatgt tggcattgct acccacgtcc
     4621 cgtgccccga cggcctttcc gcacgggtcg acagtttgga tggtcttgcg tactttggtc
     4681 ctgatagctg gcagttggcc atggagcctg gcggggagac ccttttcacg cgcgtggagt
     4741 tcacgattcc gacccacgac acgagcggat ttgaactcat tactagttgt ggtgagcacg
     4801 tttacgactg gagccgctac tttgtgacgg ccggtgatag catcgccatt gtttgtgggg
     4861 atccccgcac ctggcccgac cccgaccccg cccccgattc cgtcgttcgc cgactggtgt
     4921 gggaagagat cgagcgccag ttagccgagc aacgccgcac ctccccgccg cctcccccgg
     4981 aaggatacag cagcacttat attatcattt atcccggtga cagcgcgttg gccgacagcc
     5041 tgaccgatga cggcaagcgc cggctggcca ttatgcggag tcgcgagcgg gaaccccttg
     5101 ttgacagcga tatggacacc tgggaaatcg agggccgcgt cttccaacgg gccaaaggcg
     5161 agcgacagtt ccatcgcatt aagggacaca ccaccgagga gtggtgcgag ttagcggtac
     5221 gtatgtggga ctcattggac gccgccgacg ccgacaagcc cttggagatt ttcgtcgatc
     5281 tgcggaaacc ggagcaagag gccttcatgc gctcgtttgt cgactccctc attcctactg
     5341 acagcgccgg tatctatcgg accgtaatcc tgtaccgcca gcagaaggat ttggaggccc
     5401 atggaatccg ctaccgcaac gccagcgccg gcatgtggcg acagcgcagt cgccaaccgg
     5461 tagggaatcc ctcgccggag agccgggaac aggaggcgcc cggtaagtcc gctgacagcg
     5521 ggcgctcgtc catgttctac gaggatttcg agtgggggtc tttttcgaac acgtggatcg
     5581 cgatggacac cttgcctcaa aacggcctgg actactgggg aagcatctag gacgattaca
     5641 cgccgttcgg ctctaaagac gcctggtgcg ccgcgcaggg cgacatgccc gaaggtattc
     5701 actacgacaa ctggatggac gccttcatgg aagtgaagta tcccgtgagt actgaagacc
     5761 atactaatac gcagttgtgg ttttgggtgg attatgacac cgaggcaggt agggatttcc
     5821 ttcaggtcca gtctcagttc ggcgatctgg tctggtatgc gatctcgcct ccctacagcg
     5881 gttccccggc gacggagcgt ctgctcccgc cgcccccgaa ccgccccggc tccgggggag
     5941 cgacaaggca ccgcgaattg cccgatgcga caccccttgt ccgatagtcc gagagcgaaa
     6001 tcttcaccgg gatgacaacc gggaacagaa ggggtgaggg aaagagcggg aagagacgaa
     6061 gcggcagcgc ctggagaaac gagctgacgt gcgggaaggt caatgcgcac ttaaacggga
     6121 agaccgccat aggcgtccct gtatcagggt agtatcttac atgtaacaca ggagggtacc
     6181 atgaagtatc gaagtggatt tctgattctc ttcgggcttg ggtgcatggg cctcgtggcc
     6241 agcccggccg aggcgtggaa ggagaactac gtcggctact ttgactgctc gacgaaggta
     6301 gtcaatgcaa acccggcgac cggccaggtg acggtagccg tgacccttac gcccctgtcc
     6361 ggctatgacc gccgatgcga agcggtttcc tttgagatca ccgacatcga ggacgttgag
     6421 attctcagtc ccctgcgatg ggacctccct ttgatcgatt cgatcacgcc gctggtggcg
     6481 atgatcgatc tccgcgtgcc aacacacgac accgctggtt ttgcgctggt gacgacgtgc
     6541 gggacatgga actacaagtg gccccagttc ttcggtccgg cgggagacag catccgctgg
     6601 ttttggtctg atccccgcac ctggccaccg ctgggcgcgc ccgcccccga ttcggtccgg
     6661 gcccggctcg cccaggagcg gtcggagcgg tacctcgagt ggctccgcca gcagcccccg
     6721 ccgcctcccc cgcaaggata cagcagcact tatattatca tttatcccgg tgacagcgcg
     6781 ttggccgaca gcctcaccga gcaaggcaaa agccaactcg ccggtatgcg caagagagag
     6841 cagagcccct tagtcgacgc cgactgcgag ccgtggtcag tcgacgaccg gcacttcatc
     6901 cgccaccgcg gcgaatcccg cttccgtccc gtggagacgt tcaccctcga ccaggcgcgg
     6961 gaacgcgccc agcgcaagcg ggactctctg gaagtggctc aagcgcacat cacctacgag
     7021 gtgctcgtcg acctgcgcaa cccggcggac tcgctctggg tggccgccca cgttgattcc
     7081 ctcatcccga ccgactccac cggcttctac cggatcaaga tcaataaacg gacgtggcgt
     7141 cagatgggag aggcgggcgt cccctggtgg gagacttcga cgcggaaacc gccggtaccc
     7201 cgctctcacc ctgagccccc aaaaccgccc cgctccgagc gggacgctac ccttccgctc
     7261 cacctcgcgg acggtcgctc gcttctgttc tttgaagggt ttgagggggc atggcccggc
     7321 gcgtgctaac atagagctac aatcacacga atcaaaggag gagcgcgatg cggcgatgga
     7381 cgcgagcatg cgggatcatg tcggccggga gcatcctggc cggattgatg ctcattgtcg
     7441 ggctgctggg gccggcctgc gacgacgacc ctaccggccc cgaccacccg gatttgccgg
     7501 acggcaagga ctatgtcttc tactaccggg atcaggcgac cgggaccctg tacgggtacc
     7561 atctggtcag cgagcgtgtg gatacgttca ccgttcccta cgtggcccgg tttgggatgt
     7621 ttgtgtcggc cgacgggcaa aggctctacg ttgccggacc gcaggccacc ggggtggtgg
     7681 cgctcgccac caggacgctg gtggcggaac tgccgtatgc gcagtacccg gtaggcaacg
     7741 gcatcatctt ttcgccggac gggcggttca tggctttcct gggcagtacg ctcaccatcc
     7801 tcacctaccc ggatcacgag ccggtatttc gtgatacgat gggcgcgggg accgggtgtt
     7861 tcacgccggg cggggagcgg ctgtactatg ttccgccggg ggcggaccag gtgcgggttg
     7921 ttgagacaac cggcggggag gctcgggtgc atccccgggt gttcaatccc cgcctcagca
     7981 agattcgagt gctctccgac gagtcccgct gggtgctcta ccagagtatc tcctgcgaca
     8041 tcggcggcgt ggtatcggtc tatgacccga tccgggacac gatggaattt caggagtggt
     8101 tctacctgtc gtgcgggatg ctcgcagtgt cggccgatga tcgctgggcg ttctatgata
     8161 acccggggcc tttgctgaac cctgtgccgg gcactccact tgagttccgg gttcttaact
     8221 tggtgaccaa acggctcgat cggttggtca gcacggtggg gatagcggac gggctcaacc
     8281 cgcaggatat ggccgtgggg tggctctacg tgtcgcccga cagccgctgg ttggtggcgg
     8341 cgggcacctg gggggatcag tttctggttt tcgatgtgag gcacttaaag gtagatcggt
     8401 acatcaagct ggacggtttc tggtccgaca tgtttgccgg ccaggaagga ctctagacgt
     8461 tccgagcaca ggaggttctg caatgtacag gtggagggat ttactcgcgt gaggcgcagt
     8521 cgtggccacg ctgtgctcga cgacggtcgg ggagacccac tactacgcgg gaggtggtcc
     8581 agtcgaaatg gctaccgaca gcgggtgcgt ccaggtaatg ccggattcga tgattcccgt
     8641 agactcgttg atcaggctga tcacgcagct tgacggcgtg gtcggcctgc ctgacgagat
     8701 tccgttctcc ggcgggtacc ttgtgtgcat cctgacgacc gctactttcg attccactgt
     8761 cttggggttg gaagcgctca tctattctgc atcccccgaa ccgccccggg cccgggggga
     8821 acgagagaaa ggaggcgatg agtgcatcgg ggtgaggcca gaggcatctg ctaaccgccg
     8881 accgcaagat agagaagtcg caggcatggt cctcacacgg gtcgggccgg ctgttcccgc
     8941 tgagcctact caacgcaccg ggaacaccgg ccttccccgg gggaccggac acgtggtgac
     9001 cgatcagcgt gttgggggtc gcgttcggag ctgtccagcg gctctgtcgg cggcgccgcg
     9061 caaaaaacgg gtagcggcgt ttagtcggta ctggccggct gcgcagtctc atcaggcggg
     9121 agccgcaagg ctcccgccgc cgacgcccgc gcaagaaagg gaggatgaca tcgacgtgat
     9181 gtacaaactc gccggttacc atactgcaga taggcaaata tcgggaaagg aggtcctcca
     9241 gtgaaagaga cagccaagta ctttctttgg ggtgtagctc tcagcgctct gctgtttctt
     9301 atcgaccgcc tgaccggcat cgacgatcta ccgtacggct ggtcgctcat agcaggaatc
     9361 ggcgtcgcca ttctcgtggg agccgtgttc gcagtcgtct accggctgat ccggcggcgc
     9421 cgcgcaaaaa acgggtagcg agtctcgtgg atgctcgccc cgtacgcatt ctcatcgggc
     9481 gcgagcgacc ggggcggtgg attaggcggc atcgcacaga cgtctaatag gagcgttcgg
     9541 aaatcatgat ttcattagtc gcactgggcc tgatggtgat cggcatcgtg cttttggtcc
     9601 tcgcctatcg gcggcagcgc ccggacgcac cgcgcctcgg ctcggtggac gcgcatcagc
     9661 cgcgcacctg gcgaccagtc tggcggcagc gtcacctgtt tgccgaaaag agaggctttg
     9721 tgtgctatct catcggagca actctcattg tgagcggcgg aatgctgggc gccgtattcg
     9781 ttttctgagt cgtccggcgg ggctcttcgg gtcccgccgg cgacccaacc ccttcgcccg
     9841 ctctcctcac gccacctgcg ggcgctgcga ccggcgcctc tcgacccggt cgatgaactc
     9901 ctcgatccgc gcgttgactt ccccggcgtg cgtcaccggc cccatgtggc ccagttcggc
     9961 gatgcggacc agttccgtct ccggcatcgc cgccagaagg agctggatca cgcgccgcgc
    10021 ggtggccggg gatcggaggc cgcacaggag aagcgtcggg cgggggaact cgcggaacgg
    10081 cacgaggtcg ccgggcatgc cgcacacggc ctggaaatcg gccgtcacct tgcccatttt
    10141 ggcggccagt ccggcccgcc gccattcggg gatacttgtc caggcgcccg ctcccgacca
    10201 gtagtcgacg aaatgctgcg cggccgcctc ccgccgcccg gccgcggtgc gggttcggac
    10261 gaaccgttcg accgcgcgga tctcctccca cgcggcccgc gaattcggat catcgcgcag
    10321 cagaccgaac atcaccggct cgtacagcgt caggctcgcc accctgtcgg ggtaggcgac
    10381 cgccacccgg gccgcgacgg cgccgccgta ggagtgcccc accaggtgca ccggttcatc
    10441 ggcggccgcg atttcatcgg cgatgagcga aatctcatcg tcgagcgtca gcggccgctc
    10501 accatgccac tcgggcgact ggccgtagcc gtacaggtcg gcggccacga cccgataccg
    10561 ggcggccatc cgctcggcga gcggctgcca ctgcttggag gaactcgtgc tggcgtgcag
    10621 gcagaggatg gttgcgccct cgccgtggac caggcgatgg gctttccgta tcgaagtagt
    10681 ttctgtgctc atactccctt tctggatttc ccgtcatcta tcttggcggc gcggtttgtc
    10741 ccggtccggc cggagcggcc gccgcagggg ccggtgacga tctcagataa aatctatcca
    10801 caatacggcc cggtacgaag aaaaacaaga cttgtttcgt ccggttccgg ttatttttct
    10861 gctgcggact cccgccccgg ccgccggcgg ccgtaactct ttgcccgagg ccccgcttcg
    10921 tccccccagg ggatcttcgg cgcttgacgg acacgcggtg cggttgtatt gtataagccc
    10981 atatgaaata tctttcgaga atgatcctcc tgctgccggc cgcgttcgtg tgcctggcgg
    11041 gccgcccggc ggcgcaagac tcgctgaaga tttatctgga gctgggcggc aaggcgattc
    11101 agaacagcaa gtatgaccgc gcgatcgagg tctacacgcg cgtcctccag ctcgactcgg
    11161 ccaactatga ggcgatctcg aatctggggg tggcctacgc cagtctgggc aactatgcgc
    11221 gggcccgggg gtgtctggag caggcgctcc ggcgcaaccc gggcgatgcg caggccgcca
    11281 acaatctcgg caccgtgttc agcaacgaga acgaccggac ggccgcgatc gagtatttcg
    11341 agaaggcggt cgggctggct ccgaaaaacg cgctgttcct gaccaacttg ggacgggaat
    11401 atgcacgcga tggccaggtc ggcaaagcac ttccggttct tcgggaggct ctcgtcctgg
    11461 cacccgacaa cccgctgatt ccctactcgc tggggtcggc tttcgcctcc gcgggttccc
    11521 tcgacagcgc cgaggtctat tttgagcgca gccttgccaa aggcggcacg ggcgtcgaat
    11581 tgcactactt cctcggcacg gtcaaggacc gcctgggcaa gaaggatgag gcggtcaatc
    11641 actacaagag cgcgctggaa atggagcctg accatctcaa ttgtctccag tcgctcggtc
    11701 tgatctatct cagccaggat cggtttccgg aggcgatcga gcagtttcgc cgggtgattg
    11761 cggtcgactc gactcaatgg gccggctgga tcggcctcgg cgccagctat tcgctgtacc
    11821 gcatgatccc ccaggccgac agcattctcg ggcggctgtt ccaggccgac tccgcgatcg
    11881 ggtttcagat gctcgatttc atcggccagc agcatcgcct ccggcgcgac agcaccggca
    11941 gcaagacgcg ctagccgcgg cgccggggcg gccgggtcgc gccggttccg ctccgccgac
    12001 agcactatcc aaacgccttg ctctcccccc atactgtgtg cccccgcgcc cacatccggg
    12061 cgggcccggc cgggctgccc ccttgtcctt tgacccgtgg cgccccggca caccgtttga
    12121 atcccgcttg gacatggatt ttgtttcgaa aggagaccgt atgcacgcac gcgttcgggc
    12181 gttgtgtctc gccggcctgg tgctcctgtg ttcgccggcc gctgcccagg attggttcaa
    12241 cggagggccg gacaccctgg ccggggcgga tccggcgccg cgtccggaga cgatctccga
    12301 ctttatgcac gcggccgacc tgtggcacga cgccacgctc aagggggatc cgcggatgat
    12361 ccgcaaacgc gagctgcagc tcgtgcggtt tctcaatgag gacatcgccg cttcccggcg
    12421 gcgctacgag agctgcgcgg cgcgggtgga ggcggccggc ggcccggatt ccggccgggt
    12481 cggcgtcttc ccgccatcgg cccagcgcgc cctcgatgat ctcgcgcgcg ccgagcaatg
    12541 gctgcgggtg aaaaagcggc tcctccgcgg ctttgagcag ggcgggacgt tcgcggcgca
    12601 ataccgccag attatggatt atcgggacat tctccgcatg gagtatgatc agtggcgcgt
    12661 gctggtggcc gacggcgcct cagcggacag cgccgagacc aaatagctcc gtcgattgac
    12721 aaccagggca gccctttcga gcgccgccgc ggtttcggcc gcggcggtgt ttttctgccg
    12781 gcggtcagcc ccgctccggt ttgcgggcgg agattctcgc cgacgcgact ttcccggcaa
    12841 accgcgcgat cacgccccgg tcgatcgccc ggtccgaggc cccgcagccg caggcgtcgt
    12901 tggccagcgt gcccaacgac gcctcgtcgt acgtcatctt gtcaatcacg cggacatcct
    12961 cgaacccggc cgcgcggatc aactgcaagt attcgtcctc ttcgggcgcc ccggccaggc
    13021 accccaccca ggccgagagg tcgtcccggt actcctcggg caaactgttg gtgacgatat
    13081 cggcgatcag cacacgtccg cccggcctga gcacgcggaa ggcctcggcg aacactttct
    13141 ctttgtccgg cgagaggttg atcacgcagt tggagatgat ccagtcgacg gaggcgtccg
    13201 gaaggggcat gtgctccatt tcgccgggca cgacttcggc attggcgatc ccggcggcgg
    13261 cgagattccg ccggcaggtc gcgatcattt cgggcgtcat atccaggccg atcacgcgcc
    13321 cggcgggccc gactcttttc gccgccagga tgagatcgag tcccgcgccg ctgcccaggt
    13381 cgagcacggt ctgccccggc ttgatctcgg caaaattgac cgggttgccg cacccgaacg
    13441 aggtcacccc gtcgggcagc ccttcggtct cgcccgcacg gtagccggcc agttcctcgt
    13501 accgccgggc cgccgccggg tcaagcgctt gcggctgcgg gccgcagcaa ccgccggtcc
    13561 ctttcgtgat tgccgtccca tagtgttcgc ggaccgcttt gcggatttcc tcgggagatc
    13621 gtttgtctgt cacggccggc acctttcgta gaggccttcg ctcgtctttg cctattataa
    13681 cccgcgggag tttccccggt ccaggcgaaa aatcgcttgc gccgggtccg tttccgcaat
    13741 atatggagag cgactgaaaa gaccgttccg ttgggggtgg cgtaagaacg accgcctgag
    13801 atcatacccc ctcgacctga tccgggtaat accggcgtag ggaaacggag atgttcgcat
    13861 cgcatcccct ggccttttcc gggctaccta tgacgctcag gacggaacct tgctgaatcg
    13921 tcacatcgac gcgaggttgt tgtcatgaga tgtgctgtcc tcatcacgat catgctggcg
    13981 ccgtttgccg cggcggtgga ggccgcgccg ctgtccggca tggtgcagaa tcccgacggc
    14041 gagccgctgg ccgccgtctc cctgaccacc aacgtcgccg gcctgggaac gagcaccgac
    14101 gccgccggcg ctttcacgct cgagtgtccg ggcgacgtcc gctgggtgac cttctcctcg
    14161 gtcggctatc agccccggca gtacccggtc gacgcggtgc cggcgcgggt ggtgctggcg
    14221 tacgagtact cgccgggggg ggatgtcgtc gtgcgcggcg accgcgccca gccgggtctg
    14281 tctccgatcg cattcaccga ctactcgcgg aaagacatcg cccgcgacta cagcatcggc
    14341 gagttcccgc tgctcctgca ggcgaccccg aacctctaca cgtacaccga cggcgggggc
    14401 gccctgggct acagctcggt gcgtatccgg ggcttcgacg acaagcggat cgccgcctct
    14461 ctcaacggcg tcccgctgaa cgacccggag gaccaggccg cctatttcgt cgacctgccc
    14521 gacttcgccg ccaacatcac ggacatccag gtccagcgcg gggtcggcaa cgcgctctac
    14581 ggcgcggcgt cgttcggcgg ttcactcaac atcgtcaccg gcgccctcag ccgcgaccgc
    14641 cgcgtcaccg tgacctccgg ctacggcgag tacacctcgg gtggcgaccg ggtcagcgac
    14701 cttgccaagt cgagcatcga gtacgtgtcg gggctgatcg acggccgctg gctgtttacc
    14761 gggcgcttct ccgaccagcg ctccggcggc taccgccgcc acagctggta cgacggctgg
    14821 tcctactacc tgtcgctggc ccggctcgac ccgcgcatga cgaccgagct gtacctctac
    14881 gggggaccga tccgcatgca cctggcctac tacggggtcg atcgggcgac gcttgctgtc
    14941 gaccgccgcg ccaacccgct gacctaccgc aacgagaccg acaacttcaa ccagccccac
    15001 ttccacctcc acaacaccta ccagctcagc gagcgcgcca cactcgccac caccctgtac
    15061 tacatccagg gggaaggcta ctacgagcaa ctcaaacaac tggaggatcc ggacgacttc
    15121 gccgtgtacg gtcttgatcc gggggagctt gcggtggatc agccgagact cgtgcggcag
    15181 aagtgggtgg acaaacgcca gtgggggtgg aacccgcggc ttgatctcgc ccatgcccgc
    15241 ggccgccaca gcatcggcgg ttccgcctac tacttcacct ccgatcactg gggacaggtg
    15301 gtctgggtgg aaggtctcga agaccggtcc tacggcccgc gccagcggta ctacaggtat
    15361 gaggggagga aagtcgaggc caacctgttc gcccaggaat actgccgcct caccgaccgc
    15421 ttggccggcg aagccacggc catgctccgg taccagcggt acgacttcga tcagacccgc
    15481 atgtactcgt tccggggttt tcgctacgac gtcgactggc tgttcttctc cccccgcctc
    15541 ggtttcacct acacgctcgc cccggaaatc gcgctccata cggctttcgc cgtgtcttcg
    15601 cgcactccga ccgacgagga tatctacgag gccgatgatc ccttcgccct cccctcgctg
    15661 gacatccgtt ccgtgagcgc cgactccgcg cgctgggagt tcggcgatcc gacggccaag
    15721 gccgagcggg tgtacgactt cgagctgggc ggcgggtacc gttccgaccg gtacgcggtc
    15781 gagctcgccc tgtactggat ggagttccgc gacgagatca tcccggaggg agcggttgac
    15841 gcctccggcc ggcgccggac catcaacgcc gaccgctcgg tccacgccgg cctcgagctc
    15901 agcggcacgg cccggcctac ggaggccctg gccctgtcgg gcaatttcgc ctgggcctac
    15961 aaccggatcc gcgactacac ggcatcgctc gacctctacg acaccgactg gaactacgcc
    16021 gggcaatacc ggttcgatct cgccgacaaa accgtgcccg gtttcccggc gtacctcggc
    16081 aacgtccttc tggattaccg ctatcgcggt ctccgggcgg tctggcggca ccagtttgtc
    16141 ggcgcccagg agctcgacct cgccaacacc gacagtctgc gaatcgagcc gttccagctc
    16201 gcctcggtga gtctctccta ccggttctcc gatttttggc atctgggcgc tctcactctc
    16261 acggcccggg tcaacaacgt gttcgatcgg cgctatgagg cgggcggcta cgggtggaac
    16321 tacgcggttg caggggatgg cgggacggac gtgctcggcg gcgcggagta tttcgccgcg
    16381 cccgaacgat ggtactatgg agaagtgaag ctggagatct tctgaccggc cggcggcggg
    16441 tatgcacctc gttgattacg ccatcattgt cgcctacctg gccgtgctgt tgtacctcgg
    16501 cctgcgcaac ccgctgacgg ggaaaaacag cacgggcgag ctgatcctcg ggggccgcct
    16561 gctcgccctc ccggcctttg tcgccagcct ggtctcgacc tggtacggcg ggatcctcgg
    16621 ggtcggggag tacagctacc gtcacgggct gtccaactgg cttgttttcg ggcttcccta
    16681 ctatctcgcc gcggcgcttt tcgcggtgct cctggcccgc cgcgcgcgcg aatcgctcgt
    16741 gctgactatc cccgaccgcc tcgcccaggc gtacgacaac cgcaccgcca tcgccggggc
    16801 agccatcatc tacctcatga ctgtcccctc cgcctacatt cttatgaccg gcgttctgtg
    16861 cgagtatctg ttcggctggc cgttctggct gggcgtcgtg gtcggcacct tcttctcgat
    16921 cgtgtacgtg tacacgggcg ggttcgtctc gatggtgcgc accgatatgc tgcagttcgt
    16981 actcatgttc ctcgggttca ttgtcatgct cggagcagcc gtcgcgcgct acggcgggtg
    17041 gtcgtttctg cgctcggccg tgccggcgga ccatttcacc tggcacggcg gcaacgcccc
    17101 gttctacgtg gcgatctggt atgtgatcgc cctggccacc ctgattgagc cagcgttcta
    17161 ccagcgctgc tacgctgcga cgagcgtgcg ggtcgcccgc gtcgggatct acatctccat
    17221 cgccttctgg gccgtcttcg acttcctcac caccgtcacc ggcatgtacg cccgggccat
    17281 cctccccgag ctcgccgatc cggtgtcgtc gtatcccgcg ctggcctttc atctcctccc
    17341 gcccggtctg ctggggattt ttgcgctggg gctgttagcg acggtgatgt ccacggtcga
    17401 ttcgtacgcg cacatcgccg ccaccacgtt cggaaacgac attgtcggcc gggccgcacg
    17461 gctcaaggcg ggggggatcc tgttctactc gcggctgggg ctcgtcatct cgtccgtcct
    17521 cgccatcctc gtcgccatct tcttccgctc ggtcgtcgac atctggcacg cattcgggtc
    17581 ggtgggaacg cccgccctgc tggtgccggt ggttacctca tttgtggggc gccggcggct
    17641 tcgccccgcg tgggctttgt tatcgattgt cgcgtgcggc gggttgtcgg cgctctggct
    17701 ggcggcgagc tatgtccgcg ccgacggcgg ctactggttc gggatccagc cgattctccc
    17761 cggccttgtg ctttcgctgc tgctctttgc cctcttttcc cgccccgttg cccctggaaa
    17821 cttgccaact cccccggatt agcgtattat aaggcgtatg agcgaaccga tttacatgtc
    17881 caaagacggc gtccgtaaac tcgaggtgga gttgacccgc ctgaagtttg aggaacgacc
    17941 gaaaatcgta gccgacatca agcgcgcccg cgagcacggc gacttgtccg aaaacgccga
    18001 ataccacgcc gccaaagagg cccaggccca cctcgagcga cgcatcgccg aactggagct
    18061 caaactctcg cgggtccaga cgatcgacac cgacgccatc ccctccgacc gggtgtacct
    18121 ctatgcgaaa gtgctggtgg aggacatggt cgacggggag cagattcagt atgccgtcgt
    18181 ccccccggcg gaggccgacg tcgacaacga catcatttcc gtcgaatccc cgatcggcaa
    18241 gggtctgctg ggcaaagcgg agggggaggt ggcggaaatc gctgttcccg ccggcatgct
    18301 ccgatacaag attctgaaga tctcgcgcga ctgagcggca cccgcgttgg gccgcctgcg
    18361 ttccgcaggg agttcgatgg cgggggtttt tcccggcgcc gggaacgagc aacccatcgc
    18421 ggccctcccc cgaggcgggc aacctgcccg cgggtccgtg tgatcccttg agtccctgcg
    18481 cgaagctccc ccgattccct tgcggcgcgc ctcccccggc ggcgccgctc aatcactcgt
    18541 cgtcgtcccc gaacgtcccc tccaccgggt cttcttcgaa gtagtcctcg tcttcttccg
    18601 gttcgagtcc ggcggcaaga taagcgcatt caagggagca gtatatttcc ccctcttgtt
    18661 tcaccggttt ccctttgatc tcttcgccgc actgtgcgca ctgcatgctg gtatccctca
    18721 ggttatgacc ttcctgccac ttttgtgcgc gggtgcggtg acccgcgccg cggtcaggtt
    18781 tttcgacgtc ttctgccatc gctgatttac ggccgatgac tctgtcgggc ggccgttttc
    18841 agtccccata tagaacgggt gaatttcccc gcgcaactga tttttccatg tcgggcgcgg
    18901 tttttgacct ctcccggcgc gccccgaaag cccgcccgcc gcacgacttc cgggccggca
    18961 cgcgcggtca agcggcgaat tcatccggct gagccaccgg ccgggttcag ggagcgtccg
    19021 gtgcgtcaat ttcagtcgaa ctatgacgga attcgaccgg ctgcgcaccc ttccgccgct
    19081 ccggaggcgg ccgccgcgtt cccgcgtttt cctgttccgg cggtcccgat tgcgccggtt
    19141 ttcctatggg tagccgcgtg cggcgcggag tacggccgcc gcctgcaggc gcgcccgatt
    19201 tcggttgacc tcgcattcct gcgtcctata ttgtacgcaa gttgtgaaat cgttcacatt
    19261 aggccccaaa gcgaggattg caatgtcgat tgtcaggccc ttcaaggggc ttcgcccccg
    19321 acccgaatac gccggccagg tcgccgcgcc gccgtatgat gtcctgtcct cggacgaggc
    19381 gcgcgcgatg gccgccggca acccgatttc atttctccac gtgaacaagc cggaaattga
    19441 tttcccgccg gaccaggacc cgaccgctcc cgccgtttat gaggccgggc gcaagaatct
    19501 ccaggcgctg gtcgaccgcg gcgtcatgtg ccgcgacacc tccccctgcc tctacctgta
    19561 tcgtttgacc tggcgggggc ggagccagac cggtctctgg gcgctgacgt cgtgcgatga
    19621 atacaatcgc aacctgatca agaaacacga gcacacccgt ccggccaaag tctccgatcg
    19681 ggccgaccac atggagacac tcggcgccca agtgggaccg gtcttctcct gcttccggca
    19741 caacgaagag atccggcggc ttttcgacca cgtcaagaag gtaccgccga tgatcgacat
    19801 tacgaccgag cagggcgtgc gccacgagct ctgggtgatc agccaccgcg acacactcga
    19861 cagcctggcg gcggccttcg gccggctgcc gtacctgtat atcgccgatg gccaccaccg
    19921 gtcggccgcg gcggccgagg tggcgggccg ccgggcggcg aagaacccgc accacagcgg
    19981 cgacgaacac tacaacttct ttatgaacgt gatctttccc gacgacgagc ttcgcattct
    20041 cccgtacaat cgcgtcgtcc ggagcctcaa cggcatgacc ctcgaggagc tctttgcgcg
    20101 ggccggcgac accttcacgt tcgcgccgtc ggacgctccg gtcgagccgc agaaaccgta
    20161 cgagttcggc ctctacgccg ccggccgctg gtaccggatg agcgcccggc cggggaaggt
    20221 ggcggcggcc ggtccggctg aggccattga ctccgccgtc ctgacccggc actttctcac
    20281 gccctatctt ggcatcaagg atattcgcac cgacgaccgg atcgactttg tcggcggcat
    20341 tcgcggcgtg aacgagcttg agcgcctggt cgactccggc gccttcgcca tcgcgttctc
    20401 gcttttcccg gcgacggttg accagctgct ggcggtggcc gacgccggcg aagtcatgcc
    20461 gcccaagtct acctggtttg aacccaaact ccgtagtggc ctcgtggtga actacctcga
    20521 tgaatagatg aaagggatgg atgtatgttg attctcattt ccgacgcgtt tgatgcttcc
    20581 ctgccgggca ttttgtcccg gttcggcgag gtgaccgatg acaagtcccg tctggccgac
    20641 gccgagatcg tgctgattcg gagcaagacg aaagtgacgc gggagtacat cgactcggct
    20701 ccccgtctgc gctacgtcat tcggggcggc gtcggcctgg acaacgtcga tgtcgcctac
    20761 gcccggacca aggggatcaa ggtcgacaac acggcggatg cctccaccac cgcggtggcc
    20821 gagctggcgt tcgccctcat gattgcgctg cccaaccaca ttgccgccgc cgacgcgtcc
    20881 atgcgccagg ggaagtggct gaagaacgag ctcaagcgca ccgagctgta cggcaagacg
    20941 ctggggatcc tcggcatcgg ccggatcggc ctgtcgctgg cggtccgcgc ccgcgctttc
    21001 cagatgagag ttctcgggtg gcaccccgat gtctacttca ccgacttcgc cgagatctgc
    21061 ccgaccatgg aagaggtgct cgagcgctcc gactatgtgt cgctccacat gccgctcgtc
    21121 gattccaccc gcggcatcat caacaaggag acgctcaaga gattcaagga cggcgcctac
    21181 ctgatcaaca ccggccgcgg caagtgcatc gtcgagcagg atgtcgtgga cgccctcaag
    21241 agcggcaagc tccgcgggta tgccaccgac gtgtggaatt ccgacccgcc agagaattcg
    21301 ccgctgctgg gcgctccgaa cacgattctt acgccgcaca tcggcgcctc caccaaggag
    21361 aacatgatcc gcatcggcat cattgtcgag cggctcatcg aagactacac ggccaagaag
    21421 taaccgctca cggcataggg agaaaatctt atggctcatc gtgtctacaa tttcaatccc
    21481 gggcccgccg tcctccccct ggaggtcctc gagaccgtcc agaaggagct tctggattac
    21541 aagggcacgg ggatgtcgat tctcgagagt tcccaccgcg cggccgagta caccgaggtc
    21601 aacgaccagt gcatcgccct cgcgcgcgag ctcttcggcc tgcccgcgga ttaccacgtt
    21661 ctgttcatga ccggcggcgc ctccacccag ttcgcacttg tccccatgaa cttcctgaac
    21721 ggcaagacgg gcgcctacat cgacaccggc tcctggtcca acaaggcgat caaggaagcc
    21781 aagatcgtgg ggaaagccca cgtctgctac tccggcaagg agggcggcta caaacacatt
    21841 ccgacccaga aggaactcga tattcccgcc gacgccgcct atctccacat gaccaccaac
    21901 aacacgatct acggcaccca gttttcctcc attccggaca gcaaggggct gccgttgatc
    21961 gccgacatgt cctccgacat cgcctcgcgg cggctcgact tcacgaagtt cgacctcatc
    22021 tacgcgggcg cccagaagaa catcggcccc gccggcgtca ccctcgtcct cctgagggac
    22081 cggctgctga agacggccaa tccgaacctg ccgaccatgt tcaagtacgg cacccacgcc
    22141 gaggagaagt cgctctacaa cacgccgccg gcattcggcg tctacgtcat gaaactcgtc
    22201 ttcgagtgga tcaaacggca gggcggcctg gcggcggtcg aaaaggtcaa cctggccaag
    22261 aaggaacggg tgtaccagat aatggatctg taccccgatt actaccgcgg ccactgcgac
    22321 aaggaaagcc gctcctggat gaacatcacc ctccgccttc ccaccgagga tctcgagaag
    22381 aagttcctcg cggaggccaa ggcgcagggg ttcatcgggc tcaagggcca ccgcagtgtc
    22441 ggcggcatcc gggtctccgc ctacaacgcc ctgccgcttg aaggcgtcga caagctcgtg
    22501 gcgttcatgg agaagttcaa gaagcagaac tgacccggcc cgtgcatccg gtcaacatcc
    22561 gcggcgaggc cccagcctcg ccgctttctt tatccggccc gaccgggcgg cccgccggcg
    22621 ccgcatcggg ccgggagtcc cggttccgga tcgccggcgg caagcggggc gatcgcattc
    22681 gtggatttcg ctcgcaccgt ccctcgcgag ggtgcgcccg gccttcgatt ccggtgggcg
    22741 ggtgagggcg gccaggcggg gggagttcag ctctaagatg ttatttcgca agaggtaacg
    22801 atctcccgaa gcgaaccggc tattaacttg acaaacatac ccagaatggc tttcatagac
    22861 ggttcggacg gagtgtgcca accgcaacaa aggagttgac ttgccagccg agaagaaggc
    22921 cgctgtggtg acgtcatacc cggacctgtt cgagaagtgc tacgcgtaca ccgaaccgca
    22981 gaaggtgcag gcgatgggga tctatcccta tttccatccc ctgcagtcgg cgccgggcaa
    23041 cgaggtgatt gtcgacggta agaagtgcat catggtcggc tccaacaact acctcggcct
    23101 ggtcgaccat cccaaggtga aggaggcggc ggccgaggcg gcccgcaaat tcggttccgg
    23161 gtgcaccggt tcgcgctttc tcaacggcac gctcgacctg cacctggagc tggaggagcg
    23221 gctggcccgg ttcatgcagc gcccggccgc cctggtcttc tccaccggtt tccagaccaa
    23281 cctggggacg attgcgacgc tcgtgggtaa gaacgacgcc gtcgtgatcg accgcagcga
    23341 ccacgcgagc atagtcgacg gcacccggct ctccttcggc aaggtgtaca agttcgccca
    23401 caacaacacg gaggatctcg accgggtgct cgcccgcgtc cggcgcgaga tcacccgcgg
    23461 cggcgttatc attgtcgtcg acggcgtctt ctccatggag ggcgacatca tcaatctcgc
    23521 cgacctggtc ccggtggccg agaagtacaa cgcgcgggtg ctggttgacg acgcccacgc
    23581 ggtgggcgtt ctcggcaaga ccggcgccgg cacggccgag cacttcggcc tgatcgaccg
    23641 cgtcggcatc accatgggca cgttctcgaa atcgttcgcc tcgctcggcg gattcatcgc
    23701 cgccgactgg gaagtcatca actacatcaa gcaccgggcg cgggcgctga tcttttccgc
    23761 ctccatcccc ccgtccaacg ccgccgccgt gctggccgcg ctcgacatca tccaggcgga
    23821 accggaacgg cgcgagcgcc tctggaagaa tgcgctgcgc atgcagaagg agttccgcga
    23881 cctgggcttc aacatctgcc actcgcagac cccgattgtc ccgctcgtcg tcggcgacga
    23941 cctcgatgcg ttcgccttct ggaaagctct cttcgacaac ggcgttttca ccaacccggt
    24001 gatttctccc gccgtgccgc cgggccaggc catgatccgc acctcctaca ccgccaccca
    24061 caccgacgac cagctcgacc gcgtggtcga ggtgatcgcc aaggtcggcc gcgagaaggg
    24121 gatcattccg tagacgccta tgccgaacaa cgtccaggtc gtcgaagttg agtcgcgcgc
    24181 gcagttgcgg gatttcatta cctatcccaa ccgcctctac cgggatgatc cgtgctacgt
    24241 cgttccgctg ctgagcgagc gcctggagtt ctttgatttt gagaagaacc cgttctaccg
    24301 ggccgcccgg gtgcggctct ttctcgccaa gcggggggag gtaacggtgg ggcggatcgc
    24361 cacctgcatc aattatgccc acaacgagtt ccacggcgag aagaccgggt tcttcggctt
    24421 cttcgactgc cccgacgact acgatgttgc gtttgtcctg ctcaaggttg ccatgatccg
    24481 gctcaagatg gaggggatgg agaagatgcg cggccccatg aacttctcca ccaaccacga
    24541 aatcggcttc ctggtcgagg gttttgacga gccgccccgc atcatgatgc cctacaaccc
    24601 gccgtacttg ccggagctcg cggcccgttt cggcctgcgc aaggcgatgg acctcctggc
    24661 gtacaaactc accgatgaga ccccgatccc cgagcgcgtg gagacggtga tcgggaagat
    24721 ccggcagcgc ggcaatctgg tcgtccgccc catccgcctc cgcgacttcg acaacgaggt
    24781 cgaccgggtg aaccaggtct acaaccaggc ctggcagaac aactgggggt ttgtgccgat
    24841 gggcgacgcc gagttccgct ggatcgcccg caatctccgg caggtgatcg atcccgacct
    24901 cgccctgctg tgcgagcacg agggccgccc ggtcgccttc atcctcggca tccccgacat
    24961 caaccaggcg ctcgcccgcc tccgcggccg cctgttcccg ctcgggctgc tccggctgct
    25021 ctggcacacc aagatccgga acaagatcga cggcgtgcgg gtgctcacca tgggagtcat
    25081 tcccccctac cagcgccgcg gcatcgacat gatgctctac acggaggtct accgccgggg
    25141 cgtcaagaag gggtaccggt gggccgagct ctcgtggatt ctcgagacca acaccctcat
    25201 gcgcagcgcc gccgacgcga tcggcgccaa agtctacaag cgctaccgca tcgtcgaatt
    25261 gcccctctag ccccgccggt cggggcgcgc cggcggccgc ccgaaacgcc gttgacctgg
    25321 cgcgcgccaa ccgctatact gtccccatgc cgaacgttct gccgcgcaac acccgggccg
    25381 tgctcggcgc cgttctcctg gccgcccttc tcggcgccgc ctgcaccagc cgctaccgca
    25441 tggagctcta ctacctcgcc gacgccgagc ggtcgcgcat cgacgtcgag caggctctct
    25501 tcacgccggc gacccgcctg aatgatccgc tcgccgtccc cgacatcgtc agcggcgagg
    25561 ggagcacagc cgtgatcacg gtcggcaaac ggggcgaacg atacccgggt accggcgcct
    25621 accgggccct ctcgttcgac cagtacctgc agtaccgcat ctacctggag ttgccgagcc
    25681 ctccctggga cggccttccc gacacgatcc cggtcgtcgg ccgatcctac gtgcgcctca
    25741 tgcggttcta cgagcggccc ccggccgagc gcacctacgg gccggagacc ggcgcgttcg
    25801 tgatcgactc ggtgcgcggc agcagcctct acggcacgtt cgacggcgct cagtgggtca
    25861 acggcgccgg ccaccgcgtc ggcttcgagg gccgcgtcaa gatgcgtgtg aagtagagcg
    25921 cggcggtccc gccgtgctca ccggtgtgcc tccgccgcct tcagaaacgc ccggtacccg
    25981 agttcatcgt acaggcagaa gaccacgcgc tcgaggtgcc gcgcctcggt gcggaagccg
    26041 cgggctgctt tgaccatgat gttcgcgcag gcggtcatgg ggaatccccc cacgcctgtc
    26101 ccgaaggccg gcagcgccac cgacttgagc tgcaggcggt cggccaggtt caggctcgcg
    26161 atggtcgcct ggcggatgta gcggtcgttt gtcctgagat cctgccccat caccgcggcg
    26221 tggatgacat acttgtagcg cagccgcccg gcggtggtcg ccaccgcctg gccgggcggg
    26281 atcggtccct tcgcgatcgc ctcctgctcg accgattctc cgccggccgc cttgatcgcc
    26341 ccggcaacgc ccgctcccat ccagagcttc gtgttggcgg cgttcacgat cgcttcggcc
    26401 agagcctcga cgatgttccc tttcacgatc tcgaccgtga acgcttgatt ttcggtcatc
    26461 actccctcgc tttcttccca tcggccgatt cgttgggcgg gatgcagccc ggccgggcgc
    26521 gccgctcggc catcctcgtt tcgacccagc ggtatccctg ggccgccgcc gtcccgatcg
    26581 ccgctccgag gagcgtcccc acggcgacgt ccccgacata gtggacgccg acgaagacgc
    26641 ggctgagtgc gatcaccgcc gcgagggcga gcagcagcca ccgccaccgg ggcgcggcca
    26701 gcgcaaacag ggccgcctgt ccgaacgcat tggcggcgtg cgaggagggc atggctttgc
    26761 cgcccccgca ggcgacgagc aggtgaatgt cggtcagcgc ctggcacggc cggggccgcg
    26821 cgatgagcgg tttgagcacc gccgaggata actggtcggt gagcacgagc gcggccgccg
    26881 aaaagagcac cacccggcgc acgcgggcgt cgccgcgcag cagcaggatc accatcgccg
    26941 ccccataccc cacccgcagc agcacgtcgg aggtcaccac cggcatcagg aggtcggtca
    27001 ccgggttcgc cagcgtcatg ttgacgaaca agaagagcgc ccggtcgacg gcgttcagcg
    27061 tctcaagcat acgtctccgg cggccgctgg aggaatttcc gcagttggga cagggtgggc
    27121 atcccgccgc gcgctcccgg cctcgtgcac ttgagagccg ccgccgcgct ccccaggcgc
    27181 agccgctcgg ccatgtcctt cccccgcagc aaaccgtaca ggtaccccgc gtggaacgca
    27241 tcccccgccc cggtggtatc caccgcctcg atccggtacg cggggtggtg gctgcggcgt
    27301 cccgcctcca gtcctgtcgc gccgcggatt ccctccgtga tcacgaccgt ccccccgcag
    27361 atttcggcca gtcgcgccag cgcccggggc gtctcccgct cgccggtgta gggcagcgcc
    27421 cacgaatcgg cgaccacgag gtgatccacg agcggcagca ccggcgtgac atcgttgcgc
    27481 atcgatccga tatcgaaagt caccgtcgcc cccgcccgct ttccccaccg ggccagccgc
    27541 accgtcgcct cgatatcccg cccgtcgaga tgcaataccc gggggaccgg gtagtgcgag
    27601 agtctgagat ccctcggctc gatgaagatc cggcggtgga gaacaattgt ccgccgtccg
    27661 tcgggctcga tgaacccgta cgccgtggcc gacggcgtgc gtttcttgac gatcatgtgg
    27721 gcggtctcca cgccctcccg ggccagttcg gccagcaaca cctgtcccgg gtgatcatcg
    27781 ccaatcgccg cgatcactgc cgtccgacag cccatgcgcg ccagaccgac catcgcattc
    27841 ggcaccggcc cgcccccctg caccgtcatg tgcgtcgcat cggctttccc cccgagttcg
    27901 gggaaccgcg gaatagccgc gaggtaatcc agcgggatga tccccatccc gagggcatca
    27961 agcggtgtcg tcgtcgtctg ccgcaatggt tgctttgcct ccggatattt gcacgatgtc
    28021 gcggcggagc gccaccgccc ttgaccggcg cacggcgatg acgagccgga cgcggtcggt
    28081 gaactcggcc tcaacctgct gcgcctcgtg ctggtgcatg gccttggcca cgaggtcgta
    28141 cagggggaac tcaatcccca cttcgaggcg ctccgtaaga tagttcgccc gtgttcccgc
    28201 cgcgtcgagc gcgccggcgg ccgtctcgct gtacgcccgc gccagtcccc cggggccgag
    28261 tttggtcccg ccgaagtagc gggtcacgac cacgatcgtg ttggccagcc cccgcccgat
    28321 tacggcgtcg tagatcggcc gcccggctgt tcccgacggc tcgccgtcgt ccgagtactt
    28381 gaacatcgcc ccctcgctca gcccgaccgt gtaggcgaag cagtggtggg tggcggcgtg
    28441 ctcccgccgg cggatccccg cgaggaactc cagcgcggcc gagggtgttc ccgcttccgc
    28501 acactcgccg atgaaccgcg aggctttgac cttgatctca tgccgcgccg gccgggcgat
    28561 cgtatggtag acgtcctcca tgcggttcac acgagggcgg cgatgatctg gtccgcctgc
    28621 tccggagagt ccagcggcac cgccccatac tcggtcagcc ggtccatcgc cgccgagtcc
    28681 gacagcggac cgagccggtg gtgcgccacg atgaacacca gcttgccgat ctggctgcag
    28741 aactcgacca ggtcgagggc ctcccgggaa tcggcgtaga tctcggtcac gaccacggcg
    28801 ttgctcaggc cggcgaggag gcggttggcc gcggcgaaac cgccctgctc gtgcccctgg
    28861 tccggggcga gttccgacag gacgcctcct ccgcgcaagg cgtcgatcgc caccggcagt
    28921 tgctcggcgg cgtcgatccg gtcgagcccc gattcgatca ccgcaaacga tccctgctcc
    28981 gccgcccgcg cgccgagatg ggcggcggca tcgatccccc ggcgcagcga cgccacgacc
    29041 tggacgccgt ccgccgcgaa ccgccgcgcc agatcgaccg tcagttcgat cccctctgcc
    29101 gtggcatccg aggctcccgc gagcgtaacg gccttccgat cattcgccgg cagacttccc
    29161 cgcacgtaga gcagcggcgg cgggtcgttg agttcctcga accgccgggg atactcggca
    29221 tcgaggcgcg tcacaatgcg gatgtcgcgc tcgcgcagac ggtcgtactc ggcctgcgcc
    29281 tcgcccagat gggtctcggc ctgccggatc tgttccgccc ggtcgtcctc caggtcgtca
    29341 atcgcgtgca gcgaggacgc cgacaccgcg aagatcgcat cgaggctgcc gaaccgcccc
    29401 atcaggaggt caaacaaccg gggtcccacc ccggcgtagc ggcagagggc gaggactctc
    29461 acggcctggt tgtatccggg aatatgttct tccatgttcc aagctacggc cgcgtgcggg
    29521 caatgtcaac ggccgcgtcc ggcgcgccgg tccgggatcg ctcgcccgcc atcagatacc
    29581 ccatgcgctc ggccatcttt cgcgcgccgt ccgccgcggg ccccgtctcg gccagggaga
    29641 ggtagtagta cagggccgcc tggtcggggt cctcgtgcag cgccgtccgg agcagccggg
    29701 cggcggcggc ccgccgcccc gccgccagca gaccgacggc gtcggccgcc tgcaggaacg
    29761 acgtccgccc gaccgcctcg cgaagccgct tccggtcggc cgggtggtcg atcccggaca
    29821 ggaactggtg gaagaagccg gggtagtccg ccaccccgta ggccgtggaa tcgagcacgc
    29881 gcgccaccgt agcgaggtat tcggcggggt cgtcaaaccg taaccggccg gtcagggccg
    29941 cgccgatgat cttctcattg acccggtcaa aggccgccgc gttcacccag ttcgcgccgt
    30001 gcgccatgac cgagttgaca tcggacacgt gaatccctcc cagggcgtgg ccgatctcgt
    30061 gcagcagcgt cagcgcattc gccatcgcct gccacagcgt gtctcccggc gccgccgccg
    30121 gcagcaagtc catgtacacg cgccgccggc caagctgggc catgccgatc tcctcccgca
    30181 ctccgccgcc gaagtattgc tcgcgcgaga gcatccggtg gaaagcgacg gccagcgtgt
    30241 cggcggagac ggacacggcc cggcgaaatt cggggtaccc ggccgccagc gatccttcat
    30301 cggcggcgtt ctccggcaga ggcgccgccg agacgatgcg caaggcgacg ccgaactggg
    30361 cggcgaggtg tgtcgaggcg aggtcgacga tccggcggat gagcgccatt gccgcgcccg
    30421 aatcggccgc cagctctttc ccgaggtaga ctttcaccgg cgcggtcgcg cggagggcgc
    30481 gggccagggg ttcggccggg ggaagctcat ccccgatctg ctcgaccagc cgctgcacca
    30541 cacgttcgta gggctcgccg gcaagcgtgc ggccggggcg gcggcgggcg ttcgggtccg
    30601 gccgccccgc cgcggccgca gttcccccgg ctgccacctc ccagccgtgc gcggtgagct
    30661 gccgccgttc gtacgcggcc tcgatttgga ggcagtcctc gaccaccgct ttgtaccgtc
    30721 gccggtgggc gtcgcggatt tcacccgtcc gctcccccgc ccgcccgcgc cagcggacca
    30781 ccagcacctc tctcacccct tccccggcca gcgagtccag gagaccgcgg tcctccgccg
    30841 gcgtctcccg cagggtcacc gtcaccccgc gcccggtcat ggcggccagg tgcgcgaggt
    30901 ggttggccag cgaccgcccg agccggtcgg cgagcgccgc cggctgatcg gcggtgctct
    30961 ccgtatccat cggcgccacc accgccaccg gcgcctggac tttcggatgg ctgctgtcga
    31021 ccgcctgcac gggggaagac gcgagcagca ccgacaggaa aagggcggga cgagggacca
    31081 tagggcatat attacggccg gagggcttga aattcaagcc gcgtgttcct atcatatgcg
    31141 cgaaccgaca gcctgagagg atctatgctg cgactgctcc cgatcgtcct ggccgccctc
    31201 ctgatcgccg gctgcggcgg cgaacccaag acggaggccg agcttcgcga ggccgggaag
    31261 aaagccttcg tcaaggagga tttcgccaaa gcccgcgact acctcggccg gctcgtccgc
    31321 ctccatccct ccgaccgcga cgcgctctat ttcctcggga tggcctactc gaaagacctc
    31381 aagtatgatt cggccctcta ctacctcgag cgcgtcaaca ctctctacga ggacgaccgg
    31441 gaggcgaatc tcgagctcta ccggatcggc atgctcacgc aggatgcgaa ggttgccaag
    31501 gatgccatcg gcgccctcat ccgcacgggc gacccggccg acatgtatta cgaggacctg
    31561 gcccggttga gcctgatgct caagcagtat gcgctcgcga cccgctattt tcgcatcctg
    31621 ctcgagcgcg agcccgatca gccgcagcgc tatctcgacg ccgccaacgc cgtcgccggg
    31681 ctcgactccc tcgccgtggc ggttgctctc ctcgactccg cggcggcccg tttcggcgcg
    31741 cggccggagt tcctgctcaa caaatcgctc tacctgaccg cccaggagaa gtacggggag
    31801 agcgaggcga tcatccgcgg gctcatcgcc gccgacaccg gcaatctcgg gtacaaggtc
    31861 aacctcgccc acgtcctctc gtcgactgac agcgaggcga agcggcgcga ggcctacgcg
    31921 ctctaccacg acctgaaggg ccgggtcgac gcccgattcg cggtcgattc cctgatggcg
    31981 gatctcgccc ggaagctcga cagcgcgcag tgaccgtgcg cgggccgcgc ccggccaaaa
    32041 aaagggccgg cggatgaacc gccggccgct gccagaagat cagcttctca tttgaagcgc
    32101 ttgggtcggc gggcctccca ttcgaccacg atcgggctgg ccacgaacca ggagctgtat
    32161 gtcccgacga taatccccag caccatcgcc accgagaacg tccggagcac ctcgccgccg
    32221 aagatggtga gggcgagaat tgacacgagc gttgtgccgc cggtgttgat cgtccgcgag
    32281 atgatctcgt tgatcgaccg gttgacggcg ccggcgaact cccctttggt ccgccacttc
    32341 ttgagattct cccggatccg gtcgaacacg accaccgtgt cggtcagcga ataccccgcc
    32401 agcgtcagca gcgccgtcac cagcaggagg tcgaattcga aaccgagcag gagcatgatc
    32461 cccagaaccg ccaggacgtc gtggaaggta gcgacggctg cggccacgcc gaaccggaag
    32521 tcgaagcgga tccagatgta gacgaggatg cccaaaagcg acaggagcac cgctttctgg
    32581 gtatcgctgc ggagcgattc tccgacggcc gggccgatga tgtgttcgga cactttctcg
    32641 aacgagttgt cggtgaaacg ctgccggatc gccccctcga tggccgtgag ccgctgctgt
    32701 ccctctcctt ccacggtcgg tcgcttggtc ttgaggatga aagcgttcgg cttttcgaaa
    32761 ttctgcaact gggtgatctg ggcatcaggc gcggccgagc tcactgccat ccgcaggtcc
    32821 tcgatcgcga ccggcttttc gaacgcggcg tagatcatcg tcccgccggc gaaatcgatt
    32881 cccaggtcgg ccttgcccgt ccagaccagg accgtggcga acaggccgcc gatcaccagc
    32941 acgatggaga tcgcgaaggc gatcttccgc gcgccgatga agttgatgtt ggtctcgggt
    33001 actattgtga acatgtcgtc ttctctctcc gttctagatg ctcagcgacc ggtacgattt
    33061 gcgaatgtcg aagatctgct tggtcaccac gtaggcggtg tagagcgaga tcgccacacc
    33121 ccagaacagc gtgaccgcaa atcccttgat cgagcccgac ccgagcagga acagcgctcc
    33181 ggccgtgatg agcgtcgtca cgtgcgagtc gaaaatggcg acgaaggcgc ggtcatagcc
    33241 cgcctcgatg gccgcccgca ccgtcttccc cgtgcgcagc tcctcgcgga tgcgctcgaa
    33301 gatcagaatg ttcgagtcca ccgcgatacc catcgtgagg atgatacccg cgatcccggg
    33361 catcgtgagg gtcgcgctca gcccggccat cacggccagc aggaagaaga tgttgaagag
    33421 caggccgatg tcggcgatca gccccgagat ccggtagtag acggccatgt acaacagcac
    33481 caggcacagc cccagcaggg ccgagtagaa ccctttgcgg atcgagtcgg cgccgagggt
    33541 cgcccccacg acgtttttct cgatgatgtt caccggggcc ggcagcgcgc ccgccttgag
    33601 cacgatctcg aggttctggg catcctttat cgacgctccc ccgcccatcg tgatctgccc
    33661 gttggtgcgg attttgctgt tgatgatcgg ggcggactcc acgcggttgt ccagcacgat
    33721 cgcgagcggc ttgccgatat tcgcgccggt caactgcgcg aaccgggccg agccgtcacc
    33781 cgacagcgag aaatcaacgg tgtactcgcc gtactggccc tggccgaggc ggatgttctc
    33841 cagaaacctg cccaaaaact gcaccttccg cttcagcagg tacagcgtgt agacctcgcg
    33901 cgactcccga atctccgacc gggtcgacca cgcccactgc acgtcgatcg gcatgagcgc
    33961 ctgaacctcc gggaggccga tccaccggtc gattctctcc ttctgatcct tggccacgta
    34021 gaaccccggc cagccctcgc cgctctgcga ggagtacagc gccggcgaca gccgcgagga
    34081 cagcggtttc tcgttctccg cgtcggccgt gttctcatcg aacgcgaacg gatcctcggt
    34141 cgtgtccttc gttgcggaat cccccatcag ttcggccatg acgtccggct cctgggccgc
    34201 cgaatcggcc gtgggagcgg tcggcgcagg ctcgcccgga gtttccggcg ccgccccggc
    34261 cttcgccttc tcatagccga aaacggccga gtcgatcttg gtcaggagga gctgcgcgtt
    34321 gtccattgtc tccaggagct tgaactccag cagcgccgtc tgcccgatga gctcctccgc
    34381 ccggttggcg tcggtgtaac cggggaggtc gacgatgatg cgatcggtcc cctgtttctg
    34441 aatggtcggc tcggccaccc ccaggccgtc gacacggttg cggataatcg cgatcgcccg
    34501 atccaccgca ccgtcgcgct cggccggggc cagctccgcc atgtcgacgc ggagcacgac
    34561 gtgtattcct ccctggaggt cgaggcccag ccggatcgct ttctgctgca atttcagcag
    34621 ctcgcccggt tgttcctcct gcatccgcgc cttctcggcg tcgctcatgg tccagaggct
    34681 gaacgtgtgc cagaaggcga agagggccgc gaccacgagc accagggtca ggccgatacg
    34741 ccagttgttt ttggacatac tgtttccctt tcagtctttt atcagcacgt tcgagaaatg
    34801 tcggaactgc ggtcggcgcc ggcagagcgg cgcggggacg ggcggtcagc ccgtgccgga
    34861 gggtttggcg ccgaccagcg tgaactggcg gcccagaaga gggtgcaccg cggcggtcga
    34921 agtgcagggg gaggtgcggt cgcgcacttc gcccgcccgg cggcaggtgt gaaaatacgc
    34981 ccggtcggcg gccgccggac gcttgtctga gttgtcggtc ttgatgatga gcgggaggat
    35041 gagatggtcc gagggttccc ccggcggctg cccgttcggg gtcatgctct gctgcccccc
    35101 gtggcccgcc ggggggtagg tgcaggcgta cccgtcgccc ggcccctccc agtcggtccc
    35161 cgccgaccaa gttatcccgg cgacaaacag gcccgtgccg ccgataatca gaaagaccag
    35221 cccggtgagg ccggtccggg cgctccggcg gagcagttct atccggtccc gtttcgtcat
    35281 aagttgcgtg aatataccat gttatccggg gttgtcaacg ccaaaattcc gcccccgcgg
    35341 gccggttgac atccctcccg ccccggtata ccttagcatc ggcagatcgt cggctcgacc
    35401 aaaccgccgc gaaggcaaaa agaaccggga gttcatatgt cgaaacccgt ccccgtcatc
    35461 gccgtcctcc tcggcgcgct ttcggccctg gtggcaggcc ccgccggctc ctgccgtgcc
    35521 gccgaaacct acgagctgga caacggcatg accgtgatcc tcaaagagca gcacggcgcg
    35581 cccatggtct ccagcatcgt gttcgtccgc tccggaagca agtatgagtc ccggttcgag
    35641 aacggcatca cccactttct cgagcacctg cttttcgacg gcacggcgac cctcgcccgc
    35701 gagcagctcg acgcctcgat cggcgatctc ggcggctaca tcaacgcctt cacccgcaag
    35761 gagctcaccg cctacctggt actcctgccg aagcagtaca tcgagtacgg catgaccgtg
    35821 caggccgaca tgctcttcaa ctcggtcttc ccggaggcgg agttggcgaa ggaacgcaag
    35881 gtcgtggtgg aggagattcg ccgcgacgct gatgcgcccg gtgcggcagc cgaggcgttt
    35941 ttcacagacc gggcctacgc cggcacggat tacgctcgcc ccgtgctcgg ctacccggcg
    36001 ttcatcgaga acatcccccg cgccgccgtg atcgactact ggcggcgcta ctacattccg
    36061 cgccgcatga cgctgctggt gatcggcgat ttcgagcccg cggcgatgaa gaagacggtg
    36121 gccaacgtct tcggccgctt taccaacccg gcctcggcgg cggactccgc cgcatccgcc
    36181 tcgctcgctc ccgcccccgt cctcgaggga cagaccatct acgacacggc ggcgcaggtt
    36241 ccctccacct acctgaatct ctccctcgcc gccccgccca tcggcagcgc ggaatactac
    36301 gccatggatc tgctcgcccg gtacctcgcg atggacgccg tctcccccct catggtcgcg
    36361 ctgaaggggg gcgccgagcc gctggccacc gaggcgtcgg tgagccttgt cccctatgag
    36421 gaattctccc gccttgagat ctcggtgatc accgaacacc cggagcggcg cgactcgatc
    36481 atcaccgtca tcttcgccaa ccttgcggcg atggcgcgcc acgccgccga cccgggatcg
    36541 attgcgggac tgcagacctc ggtgcagacc gacgacatct acagcgaggc caagctccac
    36601 tactacggct tcatgatcgc accctacctc atgaccgccg gctgggattt cgtgcagacc
    36661 tacgcggaga agatggccgc cgtcacctgg gccgactgca acgccgcggc ggaccggtgg
    36721 ctggcgcggc ccgcgtacat cggcaccgtc gtgcgcccgg cgacggattc cgccgccgcg
    36781 ccctaccggc ccgaagagat gtcggccgac gaggtcctcg cccacttcgc cgaagcgacg
    36841 ttcccggaac acgagctggt cgccgggcac acgatcacct atccgccgac cgactcgatc
    36901 agcttcacgc tgaccgaccg cgcctcctac cgccgggagc agcttcccaa cggcatgacc
    36961 gtgatcgtga agacggtccc cgacaacgag gtgttcgctt tgatggtgct cggcaggaac
    37021 cgcagcgcga acgagccgcc cggccgggcc ggcatcaccg agttcgtcaa ccgctgcctc
    37081 gagcggggga ccgccacgcg ctcggcgaaa gagctggccg acgccctggc cgggatcggc
    37141 gcccaggtga cgctctacga caacccgtgg attccttacg acgaccgcta cacggcgcgg
    37201 gcgttttcgt ttttgaaatt tgagacgatc gaggcctttg cgcggaaggg atttgcgctc
    37261 ttcagcgagg tggccttttc ccccgctttc gattccgccg aggtcgagaa cgtgcgccag
    37321 gcgatgatcg gcgtgctgcg gcgccagggg acctctcccc gcgaggccgc gcgcgacctg
    37381 ttttacacca ctctcttcga agggagcgcc tacgcgcgtc cgatcatggg ggatttcgag
    37441 agcctgaacg cgatcacggt cgacgatctt cgcgcccacc accgccggat ctacgccccc
    37501 gaaaacacga ttatcgcggt cgtcaccagc cgcccggccg acgagatcct gggatggttt
    37561 gccgaggggc cgggccggtt cgccccgacc ggttttgtga gcgccgtccc cgcccctccg
    37621 caaccggtcc ggcagattat cgcggcccag gtgccgctgg agaaggagca ggtcgccatc
    37681 tacctcggcg gccccacccc gggcgcggag agccccgacg ccctcgctct ggaaatggcc
    37741 gccgggattc tctccaaccg gctctacctg aacctgcgcg agaagcaggg gctggcgtat
    37801 tcggtcggcg ccggggccga cgctgaccgg gggttcggct ggtgctatgc ctcgatgcag
    37861 accggggtcg acaactaccg ccaggctgtc ggcggaatcc tcctggagat ggacaagctc
    37921 cggtatgacg gtcctttggc gtcggaggtc cggcgcgcgc ggaacgaaat ctggggccgc
    37981 ctcatgagcg ccaagctctc cgccatcaat caggcgttct acctggcggt cgatgagttc
    38041 ctcggccgtc cgctgccgta cgatcggacg ctgctcgacc ggctgggcgc gatcgaccgc
    38101 gaaggcgtgc ggcgggtggc cgcccagtat ttccgcaccg acgcctacgt gatcgcgacg
    38161 gccggcagaa cgccggcgga aaccgccccg cccgctccgg gggagtgagc ggaggggacc
    38221 gatgtttcgg aggctgagag aactcttcgg gtcggaggac ctgctccagg cggctttcgc
    38281 gaccactctg accatgctag agatggacct ccgcatgttc gaggcctcga gcgccgcgct
    38341 ccgccggtcg gactcggccg aactcccctt tgacgtccgg caggaggacc gccgcatcaa
    38401 caagtacgag cgggaggtgc gccgcaacgt cctcacccac ctgtcggttg ccggcaccca
    38461 gaacatcgtc cccggcctcg tgctcgtgtc gatcgtcatc gacgtcgagc ggatcggcga
    38521 ctacacgaag aacatcgcgg agctcgccca gttgcacccg ggccggctcg agggcggccc
    38581 gtacgagggg gaggtggcgg agatcgaaag ccaggtgaac cggattttcc cgctcgtcct
    38641 gcgcgtcttg cgggaacagg aggccgagct cgcccgcaac atcatggcgc gggaagaccg
    38701 cacgggcaag atggccgaag cgatcgtcca gtcgctgatt tccgagaagg gccggggcct
    38761 gccggtcgcc gaggccgtgt cgctggcact ctacgcccgg tacctcaagc gcatcaacgc
    38821 ccacctcacc aacatcgcca gcgcggtcgg caatcccttc ccccgcctgg gcttccgcgc
    38881 caaaccggac cagggcgact gaacttgaga ggcgaacctg ttgggtgagg ggcgacgagg
    38941 cggaggctgg atccggccgt ctatgaggtt cccgggcgtc cccggatccc cagcccgatc
    39001 cgcgccgccg gcaggaggtg gcgccggttg acccagtgcc agatcacggc gagcacgatg
    39061 tacacggccg ccacttccat gcggatatcc tgccacacca gttggagcgc aaacagcccg
    39121 aacagcagca cgccgtccca gcgggtgagg acgagattga ccagcacggc cacggcgaag
    39181 agcgactggg ccgccgtcag gatcagctcg tgcgtctgca cgtcggtcgg cccctcgccg
    39241 tagggaacat cgagcacaaa ggggcggatc tcgccggccg agatggcgta caccagtggg
    39301 atcgtgccga ccaggagagt ccactggttg actttcgacg agatgagggc tttgagcgcc
    39361 gctccggcct gcccgcggag cgcccaggtg gcggcgacga tgaactcggg cgattccgag
    39421 gcgatcgggg ccagccactg cacgaggaag aactcatcga tcccgaggtg tcctccgctc
    39481 tccacgagcg cgtgggcgaa cggctcggcg acgaggaaaa tgacgaaccc ggagaacagg
    39541 aaaaacaggg ccgtgaacag gcggcgggga acggtcgcgg cgttggccac gatcttcacc
    39601 ggccccacca tctccggctc ctcctgcggc atctgcgccg cccgccgcgt gtacagcccg
    39661 aacagggtca cgagcacaac ggtgtcgatc aggttgaggc tccccttgag cgggatcgtg
    39721 aacgagtaca gggtcgccat ggcgagatag aagatctcca cgcgctgggt ctcatcgagc
    39781 cggacctcgc ggcggcgctt ggcgaggatg tacaggaaga gcacagccgg ccagcccagc
    39841 ccgacgagga gccggttcgc tccggtcatg ttggcgagcg cgtagtgcgc ctgggtcgga
    39901 tcgtgcgccg ctttccacgt aaagacgaag tccacggcgt actcggggag caccgccacg
    39961 agtgcgagga tggccaccgc cagcgactgc gacatgtcca tctgcgccac ctcggccgcc
    40021 cacgagagga tgaaggcggc cccgaaaatg gccaccccga acacgaccgc cgcgtagggt
    40081 gcgtagaggt gtttcccggc cgcgacgagg tacagccccg gcaggcagga caggaacgtg
    40141 ccaatcagaa tcagccacag gtaggggtcg atgtcgtagc ggtgcggtct gtctgccatc
    40201 cggtcctctc cgcgggcttt ggccgcctag ccccgaataa tcatgttcac gatgtccttg
    40261 cgcaggagga tcccgacgag gttgtcgttc tcggtgacga acacgcgcgt gaaatccttg
    40321 aaaatcatca tcgccgccac ctccacgatg cgcgtctccg gcgtcgtggt ctcgaaatcc
    40381 tcccggtaga gctgcgacac cttgatcttg tcctcgcgct tgagcagttc ctcgaacggc
    40441 tccacgtcga gcgagtagtt gagattcgag atgagcgtcc ggtaatcggg cagggcggcc
    40501 ttgatgaggt cgcggtcgct gatcacgccg agcaggcggt tgttctcgtc gaccaccgcc
    40561 agcgccgaca gacggtgccg gaacatttcg ttggcgacat ccttgagggt gtcgtccggc
    40621 cgcaccttgg ccacgtcgtg ccgcatgatg tccttgacca tcaactcctt gtcgaccgtc
    40681 accccggtgt cggccaccag tttcaccagg tcgcgcgggt tgatgatcga ccgcacgcgg
    40741 tcgagcgagc ccggcatccg ggcgaacgtc gccagccccg acagcgtctg gaggtagagc
    40801 ttggcgatcg ccgagggggt gaggagaagg cagacgacgt gcaccggcac gccgtcggga
    40861 cccccctcgt cgagcccctc ccgcgacacg ccgatgagga tgtacagttc gctgaccgcg
    40921 ttggtccggg cgtgggggaa ggcgaatccc cggccgtagg aggtgctctc gatctcctcg
    40981 cggatgcgca ccgatcgcac gatgtcgtcg taatccagcg ccacccgctc ggcccggagc
    41041 aggtcgagca gttcgtccac ggcctcggcc ttggtgtggg ccttcagatc gattcggatc
    41101 cggcgatcca tgagcaaatt cgcaagcttc acggtaaccc cttactgccg gtgtcggcat
    41161 ccttggcgga ccgttctttg aggagcgccg gcgtcaccac gccccccgag acaatgaact
    41221 tg
//
LOCUS       DAOPOE010000015        40504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1300_length_40504_cov_6.149299, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000015 DAOPOE010000000
VERSION     DAOPOE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 40504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40504
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1300_length_40504_cov_6.149299"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKM94_03675"
     CDS             <1..551
                     /locus_tag="PKM94_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOD65769.1"
                     /translation="GGVPVPTTVLVAAVGAAAWTLTEYWLHRAIMHWVPNNGWGARMH
                     FWIHGIHHEWPKDPYRLVMPPSVSLVLFFLFLALWTAVFGRSGWAFHGGFTLGYLAYD
                     LSHYFYHHRKPEREWLVRLQRHHLRHHFHPRYEERNFAITVPWWDRIFGTATPAEPSG
                     PVPGAARARSSAAEPGLRAGER"
     gene            713..1921
                     /locus_tag="PKM94_03680"
     CDS             713..1921
                     /locus_tag="PKM94_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOD65770.1"
                     /translation="MCADHQKKVHTDAWQIARHVTTLESSIIREILKISSRPGVINFA
                     GGLPDPALFPLEDMKWAMAQAIEEFGPEAVQYSLSAGITPLRKLIAERATARGTKTSA
                     ENIQITAGGQQGIELVARAFIEPGDYILTENPTYVGALQAFNYYRARYCAAEMDGEGM
                     IIDRVRKCLDRYRPKLIYTVSNFQNPTGITMSEQRRRELLDLAMSRNIPIIDDNPYSD
                     IRFTGAPVPTLKSLGGDEVVALRTFSKVLAPGLRVGWINGPADIMVHFEKVKQCTDLH
                     TNTLCQAMIYEYVKAGKLEPHIEKIKANYAEKRNLMIRTLAETFPEGAKWTEPEGGLF
                     LWIELPPRISAKDLLPKAIEAKVAYVYGEPFFPNGGGENTLRLNFATATHEEIVEGIG
                     RLAGLVKANM"
     gene            2113..2958
                     /gene="dapF"
                     /locus_tag="PKM94_03685"
     CDS             2113..2958
                     /gene="dapF"
                     /locus_tag="PKM94_03685"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD65771.1"
                     /translation="MQIPFAKYHALRNDFLVIEGPLRGSFASDPSRLAKSICDRRSGI
                     GADGILLLTPLKRGGRRIDVYNADGSWAERSGNGLRIAGLHELRRKKGSRRWTFEMGG
                     AVSEVRLRPSAPGGARMIAADMGAPDFAAAHVPVRSDLPYMIQVPLRLDDVDIPVTCL
                     SVGNPHTVLFVDDFDFDWRTLGADVERHRAFPRRTNVEFVKIVSRRKLRVAEWERGAG
                     ATGSSGTGAAAAVAAAVISGFADRACEVVFDSGSLFVDWHEETDIIELTGPVTYVGGG
                     VFEGR"
     gene            2955..4415
                     /gene="trpE"
                     /locus_tag="PKM94_03690"
     CDS             2955..4415
                     /gene="trpE"
                     /locus_tag="PKM94_03690"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOD65772.1"
                     /translation="MITYSEVRRLNARGNVIPVFARIPADLVTPVAAYRKLALGKRES
                     FLLESVEGGEKVARYSFLGFDPFLVIEGDDGRVRLIRGRRRHTVEAGPLKFVRELFRF
                     YRPVPVAGLPRFTGGAVGYFAYDTARWIEKIPDANSDPIGLPVMHLGLYRHLVVFDHL
                     KQEILLIANILHDQGKRGLRAKYDEAAATVRSMEQRLSAPARSRPGRPAGRARVTPLC
                     TEAEYRRMVARAKRYIKEGDIFQVVLSRRWRVETEVDPLTVYRRLRRINPSPYMFLLA
                     FGDAAVVGASPEMLVRIEQGTIETRPIAGTRPRGSSEAEDARRIADLLADPKELAEHT
                     MLVDLGRNDLGRVSVPGSVSVPQHMLIERYSHVIHIVSSVVGRLRRGVSPVDGLAACF
                     PAGTVSGAPKVRAMEIIDELEKHRRGVYAGAVAYIDFWGNLDSCIAIRTMVRTGGTYW
                     VQAGAGIVADSRPAREFRETQAKARALLAALTDEPA"
     gene            4484..5089
                     /locus_tag="PKM94_03695"
     CDS             4484..5089
                     /locus_tag="PKM94_03695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOD65773.1"
                     /translation="MILLLDNYDSFTYNLFQYLAELGAQVEVIRNDRMEPGAVWRRRP
                     AAIVLSPGPGRPENAGIMNRLIAEVGGSIPVLGVCLGHQAIAQVYGARIVSAPTLMHG
                     KTSAITHDRSALFAGIDSPFTATRYHSLMVAPAGLPRVFKVTARSADGVIMALQHRRR
                     PLYGVQFHPESILTAAGRRLLANFLKLCLGQGENGEGRGRK"
     gene            5177..6193
                     /gene="trpD"
                     /locus_tag="PKM94_03700"
     CDS             5177..6193
                     /gene="trpD"
                     /locus_tag="PKM94_03700"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOD65774.1"
                     /translation="MITELLQKLLRGEHLTTDEAKQAMNLIMCGEVTPAQIGGFVIAL
                     RQKGETPEEVAGFVEAMRSLSLRIELDRTRETAVDGCGTGGDGANTFNISTAASLVVA
                     AADIPVAKHGNRSVSSKCGSADLLAGLGADIDPGPEVVKENIERVGFGFLFAPRYHPA
                     MRHAALPRRELGVRTIFNMLGPMTNPAGVKRQVIGVYSRQVMYLMAEVLALLGSRHVL
                     VAHSRDGLDEFSITAPTDYIEVRDGWIAKGTLNSEDVGLPERYPGVLEGGDIARNAAI
                     LEDVLAGRESAYRDAVALNAGAMIYVADRAESIREGIVVAQRAIDSGGAKDLLDRWRA
                     ATHS"
     gene            6197..7522
                     /locus_tag="PKM94_03705"
     CDS             6197..7522
                     /locus_tag="PKM94_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD65775.1"
                     /translation="MKSAEPQLDPAPPSARSGPPFVSGLRDYLEVIRLFSRNAWLFLI
                     GSFLMGINFQVFNLLLNLYLKELGYAEGDIGLVASGRAIGMTAMAIPVAVVLSRVRLK
                     PILLATVAIFAFFSVFIVTLDRLPLLVGSAVLSGMAFAFYRVAGGPFYMRNSTARERT
                     HLFSLSFGVMILAGMAGSLLAGKTAALLTARTGDLILAYRLTLVGGIGLSLLALIPFA
                     FIQAAAPSAEENRVIFTRERFRSRGLFYFQITLVNLLIGTGAGLVIPFLNLYFSDRFQ
                     QPADTIGYYFFLVNLSMLIGSLAGPVLAKRHGMVRTVVVTQLASIPFMLVLSYGFILW
                     LAVAAFVIRGGLMNLGSPIVTNLAMELSDRSEQGLVNALLTVSWTGSWMVSTALGGQM
                     IESFGYTVAMNITIILYVISSLVFYFFFRRTEKKVDRVNGWVIMRGETP"
     gene            7519..8298
                     /gene="trpC"
                     /locus_tag="PKM94_03710"
     CDS             7519..8298
                     /gene="trpC"
                     /locus_tag="PKM94_03710"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOD65776.1"
                     /translation="MTDLLQRIVADKRAEVEALKVAHPLESFRDSLDPGSVRDFRAAV
                     SAAGRTNIIAEIKRGSPAKGMLSRVFNPVVLAAQYQQGGAAALSVLTERRHFFGHYEF
                     VRLAGKRAGLPVLCKDFIFDQYQIYHARWIGADAILLIARLLAPDQLAEFISRARAVG
                     LAALVEVHNRAEVRAALDSGAELLGVNSRDLSDFTVRLETAEELAPLIPAAVIKIAES
                     GIRAREDVRRLERAGYRAFLIGEALVTSSNPAALLQELRGV"
     gene            8295..8936
                     /locus_tag="PKM94_03715"
     CDS             8295..8936
                     /locus_tag="PKM94_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOD65777.1"
                     /translation="MIPFRVKVCGITRPEDAAVAVTNGADLIGLIFYSRSPRRVSPDA
                     AAAIITKLPPVVHRVGVFVDDPLDRVLATAARLDLDFLQLHGPYTDDDVRRARRVGFR
                     TIRLRTVRRPSDYAAFYTDPADIVMVDHPSGALAGGTGTAFDWRIRPPVPIVNLMLAG
                     GITADNLAEGVRTFQPLVVDVNSGVEEAPGVKSAEKMKRFFEVANRLRYAKAD"
     gene            8920..10122
                     /gene="trpB"
                     /locus_tag="PKM94_03720"
     CDS             8920..10122
                     /gene="trpB"
                     /locus_tag="PKM94_03720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOD65778.1"
                     /translation="MPRRTRRQPDRRGRFGPYGGRYVPETVMAALDDLERAYRGIART
                     PEFRRELGGHLRDFVGRPTPLYFAERFSERVGRAVYFKREDLNHTGAHKINNVLGQIL
                     LARRMGKRRVIAETGAGQHGLATAAACARFGLTCIVYMGAEDIVRQKPNVEKMALLGA
                     AVVPVESGSRTLKDATSEALRDWVTNVRDTFYVIGSVVGPHPYPMIVRDFQSVIGRET
                     LAQCRRRFGALPDALVACVGGGSNAIGLFAPFLRHDAVRLFGVEAAGLGLRSGRHAAT
                     LTRGKPGVLHGTYSYLLYDRDGQVVLPHSVSAGLDYPGVGPEHAFLKDAGRVEYVAVT
                     DREALAAFRLVSRLEGIIPALETAHALAWMVKAARRFGKSDRIVVNLSGRGDKDMAIV
                     GKAGKTFR"
     gene            10119..10910
                     /gene="trpA"
                     /locus_tag="PKM94_03725"
     CDS             10119..10910
                     /gene="trpA"
                     /locus_tag="PKM94_03725"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOD65779.1"
                     /translation="MSRVSRLLSARRPGHKLLAPFFTAGFPDPAVWRALVGTAAAAGA
                     DIIEIGIPFSDPLADGPAIQFSSHCALRNGLSLAVLLDGIADLRARLDLPFILMGYYN
                     PLLAYGEERFLEDAAAAGVDGLIIPDLPIEEAVSYRRAARRQGLSTVFLVAPTSTPER
                     VKLIDKSSTDFVYAVTVTGVTGTGKTFGEATDAYLRRLTAVLTRPFVAGFGVNGPAAA
                     RRMARYADGVVIGSALVEVIRSAATKRQAATEVGRFLDTVRRVLP"
     gene            10907..11197
                     /locus_tag="PKM94_03730"
     CDS             10907..11197
                     /locus_tag="PKM94_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOD65780.1"
                     /translation="MTAKGIDRLRDQIDALDRRLLSLLNERAGLVLDLARLKRADRLP
                     VFDPAREKQILEQVVGQNRGPLPGDAVARLFERIIDESRRLERTEVYDTPSE"
     gene            11208..12221
                     /gene="aroF"
                     /locus_tag="PKM94_03735"
     CDS             11208..12221
                     /gene="aroF"
                     /locus_tag="PKM94_03735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOD65781.1"
                     /translation="MIVIMQEQASDAQIQQVIAKLIDLGFDVHRSTGALQTVLGVVGN
                     TRALDARNFEILEGVQEVIRITEPYKLTGRTFRRHNTVVRTGEAAIGGERVALIAGPC
                     SVESREQIRKIAAFVRSTGTLLLRGGAYKPRSSPYSFQGLGEEGLKYLREAADENGLR
                     VVSEVMDRLDIIPALKYVDIIQVGARNMQNFALLKELGKIDKPILLKRGMSATLKELL
                     MAAEYIMAGGNHNVVLCERGIRTFESATRNTLDISAIPVLKKLTHLPVIVDPSHGTGL
                     RDKVAPMARAAVAAGADGLMIEVHYDPDQALSDGAQTLFFEQYAQLVTEIGIIASAVG
                     RSL"
     gene            12281..14083
                     /gene="argS"
                     /locus_tag="PKM94_03740"
     CDS             12281..14083
                     /gene="argS"
                     /locus_tag="PKM94_03740"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOD65782.1"
                     /translation="MARDKYKAQFAHAVAKAFRQVYPEVYREAGPAQVFDADFVYDNL
                     EKPKDPAMGRFALPVFRFGKLLRDAPPAIAAKVAAASPANGGKDMLVRVEAAGGFLNA
                     RIDATALAFETIDDILTEANHYGGSQAGKGKTELVEYSSVNIAKPFGIGHLRTTILGN
                     SLRRIFAKLGYRTVGMNYLGDWGTQFGKMIVAYRQWGGGLDLSDNAVEKLVKLYVRFH
                     QEAEGDEALNEQARKAFKDLEDGEPSAVALWQQFRDLSIAEFMRVYRRLGIEFDVITG
                     EAYLNKRMGPMIERLVRTGLASESQGALIVDLGDPQLPPLLLRKADGATLYSTRDLAG
                     AFWRWESYRFHECLYVVATQQADHFRQVFRTVALVEEAENVPPEDRFAGRLKHIDFGW
                     VRFGDANLSTRAGNIIFLEDVIDKAVALAREKIAEKNPGLVAIEDTAEMIGIGAVMFS
                     QMSVRRQKDVNFDWDEVLSFEGETGPYLQYTHARLCSLLRNYGREVSPSFDPVLLERN
                     EEQRVIEALADFPEAVADAARAYDPFYVAAHLIRLAGAFNKLYQRKNEDGRIDKIISD
                     DPALTEARIALVKAVQIVINEGLRLLGIRAPEEM"
     gene            14086..14940
                     /locus_tag="PKM94_03745"
     CDS             14086..14940
                     /locus_tag="PKM94_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOD65783.1"
                     /translation="MAASRGPVRKLAICGCGLIGGSLALDVQARGRGVEIAVWDRPGV
                     LGRIAADRRLRVRPEASFRHAVAGADVIVLAAPPTANGTLLRRLAARPEVAGALILDT
                     GSVKKPIADLAGSLTFPPGTQFLPSHPMAGREKSGSAAAAPGLFAGRPWFIDETVRLS
                     VSHRARWNWLRAAVGATPVLIDSALHDELMAELSHLPQLLATVLGAQTDPRTVELAGP
                     GLASVLRLAGSPHAVWAEIIEANRAPILAALRLYRDNLNTVMDRIARGESLRDVFAAA
                     ARSYRCLS"
     gene            14928..15944
                     /gene="aroF"
                     /locus_tag="PKM94_03750"
     CDS             14928..15944
                     /gene="aroF"
                     /locus_tag="PKM94_03750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOD65784.1"
                     /translation="MLVVMEISASREAVENVCRLIGEMGLTAHAMPGAQRTAIGVTGN
                     KSQIDADRILTQPGVKEIIHVTKPYKLVSREFIPEDTVVDVGGVPVGGPEFAVMAGPC
                     SVESREQTLAVAEAVSRLGARILRGGAYKPRTSPYSFQGLKEPGLKILAEARERYGLK
                     IVTEAMDTDTIDLVADYADIIQIGARNMQNYSLLRRAGQKRKPVLLKRGLAATVDELL
                     MSAEYIMAEGNRQVILCERGVRTFADHTRNTLDLSAIPYVKRASHLPILADPSHGTGR
                     KDKVLPLSRASLAVGADGLLIEVHCDPAKALSDGPQALTPDLFAQLMKELRLMAPVLG
                     RTMA"
     gene            15941..17281
                     /locus_tag="PKM94_03755"
     CDS             15941..17281
                     /locus_tag="PKM94_03755"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOD65785.1"
                     /translation="MRTLLPGRIADTVLRVPGDKSIGHRAAVLALLAEGPLTIRNFPH
                     SADCHASLEAVRALGATVADDGLELVLTPPPELRADPDTVINCGNSGTTARLLAGLLA
                     GSCVEVTLSGDESLRRRPMARIIDPLAAMGATIAASDGHLPMRVRGGRLLPFEYTLPV
                     ASAQVKSALLLAGLASRCAVTIRETAITRDHTEILLAELGEGLAVRKVRPVLVADPHD
                     PRKRRQEMPEPFKREITLASRAVVRGGTVDIPGDISTAAFFFAAAAISGKTITVERVG
                     LNPTRTGILEHLRGLGCRVDISERETVTGEPRGTVAVTGAPLGARRISGETTVELVDE
                     IPVIAVMAAFAEGTTVIRDAGELRVKESDRLAAVADNLKRMGAKCGLLEDGLAIEGGG
                     QLEGADFRTYGDHRIAMAFAVASLFLTGPSTLDDDECVGVSCPTFFDLLNQIRA"
     gene            17278..18123
                     /locus_tag="PKM94_03760"
     CDS             17278..18123
                     /locus_tag="PKM94_03760"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOD65786.1"
                     /translation="MTGRLSFALIGEHIAYSRSPAVFEAIFRADGVEGSFRVHEVSAA
                     AFAEEFPRLLRSGVHGLAVTIPHKERVAPFLDDLDPVARALGAVNSIAIADRLRGFNT
                     DVAGFRFALENYRAVVAGRPALVLGCGGAARAAVYALHRHFGVPSIVITSRRAETLER
                     TVERLRKGMAGLEITPAPAARLLPLDRPVGVVVNATPLGGGNHPRELPLPKAFDWTRA
                     AVYYDLNYNDDNQAVGCAREAGVAALDGAAMLVAQAIESYRRWTGRTVPFDPVFAAAF
                     PRRTR"
     gene            18142..18711
                     /locus_tag="PKM94_03765"
     CDS             18142..18711
                     /locus_tag="PKM94_03765"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOD65787.1"
                     /translation="MKKAKDPEKIFLVGFSGSGKSTIGPLLAARLGRFYVDTDRVIAE
                     KFGKDIPGIFADDGEEAFRAAETEVLGEIAGRAGVREVVVLGGGAFTIAVNRDLVRRA
                     GIAVYIQCSVRELARRMRRHADRPLLTVVPNPGESLPEATRRRVAELLEERREFYLLA
                     DMTVSSTARLPVSVVEEIVRKLENRYEPS"
     gene            18698..19834
                     /gene="aroB"
                     /locus_tag="PKM94_03770"
     CDS             18698..19834
                     /gene="aroB"
                     /locus_tag="PKM94_03770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOD65788.1"
                     /translation="MSRVDVCLGDRRYPVLIGEGGRERDLPRLVHRQARRGGRVFVIF
                     DAQVCALYGPELVARLADSGGSIHQMVLPSGERFKTLSVAAAVYDFLLRRRVARDDFL
                     LAVGGGVTTDLAGYTAATILRGIPWGAVPTTLLGMVDAAIGGKTGVNHPLGKNLIGAF
                     WQPRFVLCDVGFLPTLPDRELVAGLGEVVKYAGLAGETFLRLLESYLDGGNLLEARSL
                     SRLVRLSVAYKAGVVVRDERESGPRMALNLGHTFGHAVEQAAGFGRLRHGEAVLLGLD
                     AAVELSIAQKPSRRAGLAGYRALVRRLLGVLPRRRISAEAVMEALALDKKRAGTTARF
                     VLLDAPGRPLIGTEVPAAAVRDAVRAMLEHYAPEGGRHVQSPRR"
     gene            19812..20249
                     /gene="aroQ"
                     /locus_tag="PKM94_03775"
     CDS             19812..20249
                     /gene="aroQ"
                     /locus_tag="PKM94_03775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOD65789.1"
                     /translation="MSRVLVVNGPNLNLLGRREPEIYGTATLEDLHTRLREFAAEMKM
                     ELAFFQSNAEGEIIEFIQREGPGADGLVINPGALAHYSHALRDAITAVGIETVEVHLT
                     NIYAREEFRRHSVIAPAARGQVVGFGMYGYAMALSYFADTSDK"
     gene            20272..21870
                     /locus_tag="PKM94_03780"
     CDS             20272..21870
                     /locus_tag="PKM94_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOD65790.1"
                     /translation="MKRLVLLLIGLSLLAAACREQPVSRDDVFLVMDSLEHKFDWLGY
                     RLQQETWEIYSTGQSDSLDFYQGLLRYTVADPNLLRTFRQGRLWVTENEDVRRLDLLE
                     AALMRAQVEVAEPVASLHDSLVQIDVTFRAPFDGQNRTQNYLYRTYRSDPDRSRREAA
                     FRAYHRIGDLLADGLARLIRLRNREAAKFGQQNYFSLLFSQQGFDEARYLATLDQLDS
                     LSLEPYRRILANAGEKLGSGPVEIWDLSYAYADINRRGDAYFPADRQFSQIARSLEAL
                     GYDLDKLPIYFDLQPRSDKTQLAYAVPLRPPHDVRVLANVTDGYLSARTLMHEIGHAL
                     HFAHIRQERQLFVDCVSEAWTEGMAQIFASLLDDPQWLVQYAGMTPETAAEFLRARAE
                     QEIIYLRTTLMRLRWEYEAYRNPDQDFNTLYWDLFARYLLLPRHDDIRPWATIIQYTT
                     HPVYLHNYLYADMIASQTLASLHTVYGPLIDNPSLRSFLNQNYFRFGARYDWRDLLER
                     GTGQRLDQNYLIRRLCIEPPAAAF"
     gene            21970..23319
                     /locus_tag="PKM94_03785"
     CDS             21970..23319
                     /locus_tag="PKM94_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD65791.1"
                     /translation="MTRTETITSGPISGTIISLATPVVLAQFMEFALASTDYFWVGRL
                     GPFAQDALTTSMVTVWTMFSAVSVISVGVAALVARHIGAGNPGQAAFFARQGMGMGVL
                     FAIAAALAAWVLTPPFLRFMQAGPQTLPMAQTYMRTVYLFALCFFLLEVSYAVFRAAG
                     DTRTPTIIAAITIVLNIGLDPLLIFGWGPVPAMGVAGAALATGLSVTIAAALALTRLA
                     GGHLGFRLGPVRAFRPDFRQMLRIARIGLPIASQQFIFVAVYWFLIKYVHAFGPTAGA
                     AMGIGNRMESFSYLICSGFAVAASTMVGQNLGAGKPDRAARCAWGATGIAVAVTLAVA
                     VVFVSLPRAIASVFTDDPEVLRMAADYLVILGLSQFTMAVEIVLEGAFSGSGDTLPPM
                     LVSVPGSLARIPLAYLLAFTLGWGINGVWWTLTITTTVKALVLAYWFSRGRWKRKEV"
     gene            23398..24171
                     /locus_tag="PKM94_03790"
     CDS             23398..24171
                     /locus_tag="PKM94_03790"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOD65792.1"
                     /translation="MPRRYARVRLPDRRVVWARLTDDDMVAPLAQAPWRGEAPAGPAV
                     PLAQATLLAPTEPTKIIGIGLNYHAHVQASFSADKAPDHPLIFLKPPSSVIGPGEKII
                     HPPQSQRVDYEAELGVIIGKEARRVPADRAADYIFGLTCVNDVTARDLQKTDGQWSRA
                     KGFDTFCPVGPWAVCGLDYSDLLVEGILNGEIMQSGRTSRMIFKIPELISYVSSVMTL
                     LPGDIISTGTPAGIAPMKPGDRIEVRVEGIGSLVNQMAE"
     gene            24226..25416
                     /locus_tag="PKM94_03795"
     CDS             24226..25416
                     /locus_tag="PKM94_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOD65793.1"
                     /translation="MTERLYYTDPDLLRFEARILDAGREGGRYFAVLDRTAFYPTSGG
                     QLFDTGILNSVSIVDVTESSDGTIRHYSPQAIGEVGAVVSGAVDAARRKRHRRQHTAQ
                     HILSHAFLQLHGCETVSVHLGEEYGAIEFDTGALSDDQLLAVEERAEEIIFADLPVDI
                     LFVPPEQIATIPLRRPPKKEGILRVIRIGDIEYSACGGTHCRRTGQVGLVKIIGVDKM
                     RGHALVNFLAGDQARDDYAARLEVTARLTRHFTCNVRDLGDKVLKLEAEKRGLHQEVV
                     RLQRELLPSQADSLAGQATAVGERLVLVAELPHYDPKLALQLAALLAERINGLAVLLV
                     ESRLYIAVGPGSGLHAGNIVKALGAATGLRGGGGPLQAQLGGADPAQLTTYRALAEKL
                     VREA"
     gene            25406..28945
                     /locus_tag="PKM94_03800"
     CDS             25406..28945
                     /locus_tag="PKM94_03800"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOD65794.1"
                     /translation="MRRNRAIVTAILFLLAAAGAAAQNRQGFVLERSNQLEVVWENER
                     YVTYVDGEVKFRTETGAVYCDSATFVRGEYAKLRGRVLIDDQAYMLRADSVYYDLTRE
                     TALALGSQVQLWSYADSLYAAGVHAFYDRPAQHFEMQERPTVYLGYPDTAAMVEVVAN
                     TVNYFAPARRAEAVGRVVITSQDIAAAAECAVMLMDRNTLDLYDGPTAKRGRSTMTGG
                     FMSIAFGEDQLREIDVIDSARGEFIEPIDSAETDVDRSILAGSRIKMFFDEGDLRQVL
                     ASGQAYSWYYPSPRGTREFHENTVSGDTIRLFVREERLEKVAVVGGAAGTYITGTVSD
                     SSAAGADTAAAPETAVSAAVRDSTAAATAATRLDTVDYRAEHIVYSLVDSLITLDVRS
                     EVKSGAMELRAYDVDFDTRRRVVEAFSAEITPDAADRDYYSLAERLQPAAIPVILKDG
                     ADELHGDYLEYSIDTEKGRIVQTKSEFEQGYYYGGKLFRQKRDVYYIEDGYYTTCGAD
                     EPHFHFYSSRMKLMQDNKLIARPVVFYLGRIPLMAIPYYVFPLKKGRHSGFLPFTFGR
                     FERGERYVNNLGYYWAASEYWDYRGAVDYHDLSRTITFKSEVQFRKRYLLDGYLRGEY
                     RRQTQYNRRVALENSPTSYSFSGAYNHTLSPSFDVRSSGSYVSTASYYTDYSNSLAER
                     LNRNLVSQVNFSKKFGRLTSVTGSVQHTENLDLEQRTDQLPSLSVSLPPIYPFGGSST
                     NDEGETVTRWYNQFILRYNPTLLNYSTRSTILADTIHTLVDTLIDTTFVDDTTIVAID
                     TTYRAVEIDSLMSRSRRHYLKVQHNPSITLPTIRIIPYVPIVPSFSYSETWFKIFETD
                     QSQAAGIDASEFYRTYSYSAGVSANTKLYGTVYPGLFGLLGLRHVLEPSVGYRWSPDI
                     QRYPEVRSFAGGGAASSKSQSIGFGLGHLFQAKVRTGETEKNIDLLSVRSSFAYDLER
                     DFRPLSQMTTSFQTSTIPRLNISGDMRHTFYNPYDDSATTPKLLSPWLLDFDLNLSFD
                     VMGRTFLFTDEAEPGVPLGVDSASALAGPELPMPSAARAGWNLAVTYSFSESGRHRSY
                     SKSSFVNITLGFGLTPSTSVNYNQRYDLVEGRTISSQVNIVRSLHCWTGSLFWVPVGS
                     NTGFGFKLWVNELPDIKLDSNHDSFISTDVLRR"
     gene            29073..29351
                     /locus_tag="PKM94_03805"
     CDS             29073..29351
                     /locus_tag="PKM94_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD65795.1"
                     /translation="MTKDEMIAHIAEASGITKRQATSALDAFMDGVTRQLKKNEKISF
                     AGFGTFAVSQRKARTGRNPQTGEAIKIPATRVPVFKAGKNLKEAIKKK"
     gene            29454..31826
                     /locus_tag="PKM94_03810"
     CDS             29454..31826
                     /locus_tag="PKM94_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOD65796.1"
                     /translation="MAKKKSARRSRKSGGFSERARQALGVVFILLALLLLISLVSHDS
                     LDDLRVTGDADTHLAVFDIQFRNQAGLIGAYLSFLLMLLAGWLSFFLPVGLGVAALRL
                     FAPERAKRWRFNVLLLFAISVLGTMLVNIHRLAAHTLEADPDAVGGYVGEKLTLLAIK
                     LIGELGSYVLLGGSILLLLVLYTSITPVLAKALPLPGGETVRKIYTVMRNAAVTIFSF
                     RWLVSQSEDEEDEAAAGESEPAAPAPSRERPPDSAEFEDLLDEQIEADAVPDGADPAA
                     DREPRPKKPRAKPAASPSDEPVTRGSIDYRFPPLSCLARDPNPANASVTEEELKATSR
                     MLKETLETFGVSVSGEIEYYPGPVITRFELKPAAGVKVNQIVNLSDDLALALKAKRIR
                     IIAPIPGKAAVGIEIPNRKAQLVYLRDILEDDIFARTTMRLPLALGKTTAGKPYAVDL
                     AKLPHLLIAGATGSGKSVCANVLITSLIYKLHPHQIRFIMIDPKMLELTAYSGIPHLG
                     RPVVTNPKRAEKVLSDAVVEMEQRYRRLAAASVRNIEDFNRKQEADDQRLPYIVILVD
                     ELADLLMSSTSSRMELLITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPARLAF
                     QVSSKVDSRTIIDANGAEKLLGNGDMLFLYPGQPEPIRIHGAYLTGDETEAIVTFIKA
                     QELPMLKLENISQATGEAADVEEVDAGDPLFREACEVVIRHKQGSVSLLQRRLGIGYQ
                     RAARLIDKLEKAGVVSPFDGSKAREVLVDKSYLETLAATEGAPVAEQETN"
     gene            31853..32458
                     /locus_tag="PKM94_03815"
     CDS             31853..32458
                     /locus_tag="PKM94_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOD65797.1"
                     /translation="MTKVLISVAVLACSAAAFASDDPFEGAKQDLARAACVQVQFLSV
                     LESDVFDVVDSVPGTAIIAADGRFAVSVGSEEYLYDNERLYTYSAQTDQVIIEKPSGD
                     LFVGNEIALVTRLDRYYRTAPVAAGREYRLTRTDTTGTQLPDELTVLLRPDSSAIEAF
                     LYRDINGDLNRVVLLRQNLSEACADSAFIPAFPESAERIKL"
     gene            32466..33797
                     /gene="rimO"
                     /locus_tag="PKM94_03820"
     CDS             32466..33797
                     /gene="rimO"
                     /locus_tag="PKM94_03820"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOD65798.1"
                     /translation="MKFYIHKLGCPKNDVDADYIAARLIDAGHEPVCDPLAADSIIVN
                     TCGFILPAKEESITEILRLGQLKKEGRLRALFASGCLSQRYGDELLKEIPELDGAFGH
                     GALDSLAAAVDGAPRFDRAVRVEPRRLAYLDWRDRFISDPYPWAYLKISDGCDRGCTF
                     CAIPGMRGRFRSRPLESILREARFLADRGKRELILVSQEATLWGYDLPGRPDILSLLR
                     ALDGLAGVRWIRLMYLYPAALTTELIEYLADDGTKALDYFDLPLQHISDDILRAMRRR
                     GDRRAIESQLRAIREIAPGGTVRTTLIVGFPGETERHFAELREFVERFEFDRLGVFAY
                     SPEEGTPAAEYPGQVPERIRQRRVDDLMTVQQEIAFAKNNSLIGRILEVIIDSAPADG
                     PAVGRTRGDCPEIDQEISVCGERLAAGTFCRVRVEAADGYDLRGCTVGVVA"
     gene            33794..34438
                     /locus_tag="PKM94_03825"
     CDS             33794..34438
                     /locus_tag="PKM94_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOD65799.1"
                     /translation="MIQFEKLGIFLSKPVAFVLVVIYLLQSGLLVYLVKNKYDLERQI
                     SFQQTRIGTLEEKLQIFKAIDDFQIGFSDEEINRLTDVIYEESKKYEYDPLFMLAIIL
                     TESSFKKGQTSPAGAQGLMQVMPFVGRDVAGRSGVAWQGDSTLFQPDINIRLGALHLF
                     EQILKFGDVKQALVAYNLGETRLRGILRDKQPVPKTYLNKVLENYKMLKEAYRV"
     gene            34435..35370
                     /gene="bamD"
                     /locus_tag="PKM94_03830"
     CDS             34435..35370
                     /gene="bamD"
                     /locus_tag="PKM94_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOD65800.1"
                     /translation="MNRITAAGALAGLLLVAALLASCGAGSRSLSQLDARQLFDEGQR
                     LYEDGKYRQAIEYFQTVVFNYPGHAVVDTAQYYLGLAYYGNEDFELAALEFDRLARNY
                     PASAFFANAIFMRGVAYFDSTPKNPGLDQTELEKAIAVFEDFLIDFPESPLVPDVQKY
                     LAAARTRFAKKYFNAGIVYERLGAFKAAKVYFQKVIDEYTDTDLAARAAFHRADMDLR
                     LKDFAAARTGFESFLAAFPESDLAGKARKRAVESAYRQCRLSYDKGDTAEARQCLRQF
                     RESYPANGYEDDVNEMLEALDKHPAGSDTTRHAES"
     gene            35357..35965
                     /gene="nadD"
                     /locus_tag="PKM94_03835"
     CDS             35357..35965
                     /gene="nadD"
                     /locus_tag="PKM94_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOD65801.1"
                     /translation="MRSPEQGGVWGVLGGAFDPVHSGHLALAREILRARNLAGVLFVP
                     AYRHPFKGDRARASWPHRVAMLRLALAGDDRLRVCEIEAEQELSGYTLDTLRALKKAY
                     PQAAFRFLVGADLLDQIDQWHSPEELLRETPVLAGARPGYQLKVSPGLPADRIELVPA
                     ETPAVSSTDLRALIGRGAPRPRLLAWLPAPVLDYIEKEGLYR"
     gene            35962..38688
                     /gene="polA"
                     /locus_tag="PKM94_03840"
     CDS             35962..38688
                     /gene="polA"
                     /locus_tag="PKM94_03840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOD65802.1"
                     /translation="MTPDRKTVYLVDGSAIFYRAYFAFLRNPLINSKGENTSAVFGFV
                     NSLLKVIRDEKPDYLAVAWDSKAPTVRHERYEEYKSTRAKMPEDLVAQLPAIREAVEA
                     FRIRHFEMEGFEADDIIGTLASRCAKAGFDVWCVTGDKDFFQLVTDRIRIYTPQPGAD
                     QAVKMGREEVKAKFGVYPEAVIDKLALMGDSSDNVPGIPGVGPKTADALLEQFGSLEA
                     VLAGANRIKAKAVRARVEAHADLARLSKELVTICTDVPIACDLEQLRFREPDHAKLVE
                     LFQRLEFNRLLKQLVPEAAAAAGVTDEAPGATKPGYHVVAALKDLKTLLKKLSTCRKV
                     AVDTETTSLNPLAAELVGISLSARANEAYYLPLGHTGEGAVNLPLPEALAEVKKLLEN
                     PDVQKFGQNIKYDLAVLRKYGIEIEPVRFDTMLASYVIDPSSRQHSLDALALQHFNHR
                     KTPITDLIGAGKKQISFAQVPVDKACDYSCEDADYTYRLRGKLAPVIDQLGMNSLYYD
                     IEVPLIKVLADMEREGVRIDVSFLGELSVQMERRLEEIRREIYRIAGTEFNINSTQQL
                     AHILFEKLGLPTKGKTTKKTGFSTDVRVLEELAALHELPARILDFRQLTKLKSTYVDA
                     LPKLVDGRTGRVHTSFNQTIAATGRLSSTDPNLQNIPVRTDEGREIRKAFVPRDEGHT
                     LLVADYSQIELRILAHMSEDSTLIEAFRRGEDIHARTAAEVYGVEIGQVTPEQRRAAK
                     TANFAVIYGVTAYGLAAQSALDVGQAKAFIATYFERYPGIRRYLEATKEFARARGYVT
                     TMFNRRRTIREINDRNVNVRQFAERAAINTPIQGTAADMIKKAMIRIHGRLRATRSRM
                     ILQVHDELVLDVPREELDEVREIVRDGMEQAVSLKVPIVVSLGSGPNWLDAK"
     gene            38736..39362
                     /gene="coaE"
                     /locus_tag="PKM94_03845"
     CDS             38736..39362
                     /gene="coaE"
                     /locus_tag="PKM94_03845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOD65803.1"
                     /translation="MLIGLTGLIGAGKSTAARILESFGAVVLDADEIGREVVEASLPL
                     RHRLAEAFGADVLTVDLDLDRKKVAERAFAGEESRRRLNEIVHPYLLHELDSRMRRLA
                     KRCPVVVVDAALLTEWRLHQRMDYTIVIDAPEQVRLDRLAARGMAPSEARAIERLQPS
                     PQDLRVCADFLVVNDGSTDRLAATLSDIWEMITRSKRGDLHEEPDTAP"
     gene            39337..40497
                     /locus_tag="PKM94_03850"
     CDS             39337..40497
                     /locus_tag="PKM94_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOD65804.1"
                     /translation="MKNPTPHPENARCDYRVDELVERARYMRGLNEIALCSARSGHSG
                     GTLSVMDIAAALYLRIARHNPTDPDWPDRDRIIWSAGHKAPALYTALAVAGYFPEPEL
                     MKLRMLDSPLQGHPHRLDLPGVEISSGSLGQGFSVAVGVALAARLDGKDYRVFVISSD
                     GEHQEGSMWEAAMAAAHYRLDNLILIVDKNRLQIDGPVAEVMNIDPLADKYAAFGWHV
                     READGHAMAEIVEALDWARNRNDSGKPVALIFHTVKGKGVSFMENVVGWHGKPPKLDE
                     LERLLAELGLGDTFAVSDLIDYGVRHQIGVDARLNDELPAFSRDFWWNAGPAMRVDMD
                     PTRKGFGRALEACGSDERIVCLGADISDSICISDFHKNHPERRRRTWIYQTE"
BASE COUNT         6657 a        13903 c        12821 g         7123 t
ORIGIN      
        1 ggggcggcgt cccggtcccg acgacggtcc tcgtggcggc ggtcggtgcg gcggcctgga
       61 cgctcaccga gtattggctc catcgtgcga tcatgcactg ggtgcccaac aacgggtggg
      121 gcgcccggat gcatttctgg attcacggca ttcaccacga gtggcccaaa gatccgtacc
      181 gcctcgtcat gcccccgtct gtcagcctcg tcctgttttt cctctttctc gcgctctgga
      241 cggcggtgtt cggccgttcc ggctgggcgt tccacggcgg cttcacgctc ggctaccttg
      301 cgtacgatct gtcgcactac ttctatcatc atcggaaacc ggagcgcgaa tggctggtgc
      361 gcctgcagcg gcatcacttg cggcaccatt tccatccccg ctatgaagag cgcaatttcg
      421 ccatcacggt gccgtggtgg gatcgcatct tcggcaccgc cacccctgcg gagccgagcg
      481 gtccggtgcc gggggcggcc cgcgcgaggt catcggccgc cgaaccgggc ttgcgggccg
      541 gcgagcgttg acttcccctg cggtgcggcc ggcgcgcggt tggcccgctg agtgaatcgt
      601 ccccgtgggc gatgattcct ccttggattt gagttccctc gatcctatct tggcgcaagg
      661 agtccggccg ccgcctcggc gggggggcgg cgaagaagaa agggagaacc gcatgtgcgc
      721 cgatcaccag aagaaggtcc atactgacgc gtggcagatt gcccgccacg tcacgaccct
      781 cgagtcctcg attatccggg aaatcctcaa gatttcctcc cggccggggg tcatcaattt
      841 cgccggcggg ctgcccgacc cggcgctgtt tccgctggag gacatgaagt gggcgatggc
      901 gcaggcgatc gaggagttcg gcccggaggc cgtgcagtac tccctgtccg ccggcattac
      961 gcccctgcgc aaactcattg ccgagcgggc caccgcgcgc ggcaccaaaa cctcggcgga
     1021 gaatatccag atcacggcgg gcggccagca ggggatcgag ctggtggcgc gggcgtttat
     1081 cgaaccggga gactacattc tcaccgagaa ccccacctat gtcggcgccc tgcaggcctt
     1141 caactactac cgcgcccgct actgtgcggc ggagatggac ggcgagggga tgatcatcga
     1201 ccgcgtgcgg aaatgcctcg accgctaccg cccgaaacta atctacacgg tgagcaattt
     1261 tcagaacccg accggcatca ccatgtccga gcagcgccgg cgggaactgc tcgacctggc
     1321 catgagccgc aacatcccga tcatcgacga caacccgtac agcgatatcc gcttcaccgg
     1381 cgccccggtg cccacgctca agagtctcgg cggcgacgag gtggtggcgc tgcgcacctt
     1441 ctccaaagtg ctcgcccccg gcctgcgggt gggctggata aacggcccgg ccgatatcat
     1501 ggtccatttc gagaaggtca agcagtgcac cgacctccac accaacacac tctgccaggc
     1561 gatgatctac gagtatgtca aggccggcaa gctggaaccg cacatcgaga agatcaaggc
     1621 caactacgcg gagaagcgca atctcatgat ccgtacgctg gcggagacct tccctgaggg
     1681 agcgaagtgg acggaaccgg agggcggtct cttcctgtgg atcgaactgc cgccgcgcat
     1741 ctcggccaag gatctgctcc ccaaagcgat cgaggcgaaa gtcgcctatg tctacggcga
     1801 gccgttcttc cccaacggcg gcggggagaa caccctgcgt ctcaatttcg cgaccgcgac
     1861 tcacgaggag attgtcgagg gtatcggccg gctggccggg ttggtgaagg ccaatatgtg
     1921 agacacaggc cggtcggggg cgccgggttt ctccttttgc ctttggcgaa tgccggaccc
     1981 cgagccagcg gtcacggcga cggattgagg ccgcagcgcc ccgggcggag tcgaacggga
     2041 gggggagcgg ttttaccggg cggcccgctg taaaatcttg cccgtcggtc tcaattggcg
     2101 taattaccgc ccatgcaaat accgttcgca aaataccacg cgctgcgcaa cgacttcctg
     2161 gtgattgagg gaccgctgcg cggttctttc gcctccgatc cctcccgcct ggcgaaaagc
     2221 atttgcgatc gccggagcgg catcggggcg gacggcatcc tcctgctcac cccgctcaag
     2281 cggggaggga ggcgcatcga cgtctacaac gccgacggat cctgggcgga gcgatcgggc
     2341 aacggtctgc ggattgccgg cctgcacgag ttgcggcgga agaaggggag ccgccgatgg
     2401 actttcgaga tgggcggcgc ggtcagcgag gtgcgtcttc ggccgtcggc gcccggcggc
     2461 gcgcgaatga ttgccgccga tatgggggcg cccgatttcg ccgccgccca tgtcccggtc
     2521 cggtcggacc tgccgtacat gattcaggtt ccgctccggc tcgatgatgt cgacatcccg
     2581 gtcacctgcc tgtcggtggg caacccgcac acggttctgt tcgtggacga ttttgatttc
     2641 gattggcgga cgctcggcgc cgacgtggaa cggcaccggg cgtttccgcg ccgcaccaat
     2701 gtcgagttcg tgaagattgt cagccgcagg aagctccggg tcgcggagtg ggagaggggc
     2761 gccggcgcca ccgggtcttc cggcaccggc gcggcggccg ccgtcgcggc ggccgtgatt
     2821 tccgggttcg ccgaccgcgc gtgcgaggtg gttttcgact ccggctcgct ctttgtcgac
     2881 tggcacgagg agaccgacat aatcgagctc accggccccg tcacttatgt cggtggggga
     2941 gttttcgaag ggcgatgatc acctacagtg aagtccgccg cctcaatgcc cgcgggaacg
     3001 tgatcccggt gttcgcacgc atccccgccg acctggtgac accggtagcg gcctaccgga
     3061 aactggcgct cggcaagcgg gagtcgtttc tgctggaatc ggtcgagggg ggagagaaag
     3121 tcgcgcgcta ctcgtttctc gggtttgacc cgtttctcgt aatcgaagga gacgacgggc
     3181 gggtgaggct gatccggggt cgccgccgcc acaccgtgga ggccgggccg ctgaaattcg
     3241 tgcgcgagct gttccggttc tatcgtccgg ttcccgtggc gggcctgccg cgcttcacgg
     3301 gcggcgcggt ggggtacttt gcctacgaca cagcccgctg gatcgagaag atccctgacg
     3361 ccaactctga cccgatcgga ctgccggtca tgcacctcgg tctgtaccgc cacctcgtgg
     3421 ttttcgacca cctcaagcag gagatcctct tgatcgccaa catcctgcac gaccagggga
     3481 agagggggct gagggccaag tacgacgaag ctgcggccac ggtccgctcg atggagcagc
     3541 ggctgagtgc gccggcccgg agccgccccg gccgccccgc cggacgggcc cgggtgacgc
     3601 cgctgtgcac cgaggccgag taccgccgca tggtcgcccg cgccaagcgc tatataaagg
     3661 aaggggacat cttccaggtc gtcctctccc ggcgctggcg ggtggagacc gaggtcgatc
     3721 cgctgaccgt gtaccgccgc ctgcgtcgca tcaatccctc cccctacatg ttcctcctgg
     3781 cattcggcga tgccgctgtg gtcggcgcct ccccggagat gctcgtgcgg atcgagcagg
     3841 gcaccatcga gacccgcccc atcgccggca cccggccgcg ggggagcagc gaggccgagg
     3901 atgcccgccg gatcgccgac ctgctggccg acccgaaaga actggccgag cataccatgc
     3961 tggtcgatct cggccgcaac gatctgggcc gcgtcagcgt cccgggttcg gtcagtgtcc
     4021 cacagcacat gctcatcgag cgctactccc acgtgatcca cattgtgagc tcggtggtgg
     4081 ggcgtctccg gcgcggagtg agcccggtcg acggtctggc cgcctgtttt cccgccggca
     4141 ccgtctccgg ggcgcccaaa gtgcgggcca tggagatcat tgacgaactc gagaagcacc
     4201 gccgcggggt ctacgccggg gcggtcgcct acatcgactt ctggggcaac ctcgacagct
     4261 gcatcgcgat ccggaccatg gtccgcacgg gcgggaccta ctgggtgcag gcgggggcgg
     4321 gcattgtcgc cgattcccgg cccgcgcgcg aattccgcga gacccaggcc aaggcccgcg
     4381 ctctgttggc cgctctgacc gacgaaccgg cctaacggtg ttcccccatt tgcccgatca
     4441 tatgcgtacc atcaccgatg aaccccttca tggagtcggc caggtgattt tgttgctgga
     4501 caattacgat tcgttcacct acaacctgtt ccagtacctt gcggagctgg gcgcgcaggt
     4561 ggaggttatc cgcaacgacc ggatggaacc gggggcggtc tggcggcggc gtccggccgc
     4621 gattgtgctg tcgccgggac cggggcggcc ggagaacgcc gggatcatga atcgactgat
     4681 cgccgaagtc ggcgggagca ttcccgtcct cggcgtctgt ctgggccacc aggcgatcgc
     4741 ccaggtctac ggcgcccgga ttgtctccgc cccgacgctc atgcacggca agacctccgc
     4801 catcacccac gaccggtcgg cgctttttgc ggggattgac tcgcccttca cggccacgcg
     4861 ctaccattcg ctcatggtcg ccccggccgg tctgccgcgg gtgttcaagg tcacggcccg
     4921 gtcggccgat ggtgtcatca tggcgctgca gcaccgccgc cggccgctct atggggtgca
     4981 gtttcacccc gagtcgattc tcaccgccgc cggccgccgg ctcctggcga acttcctgaa
     5041 actctgcctt ggccagggcg aaaatggcga ggggcgcggc cgaaaatgaa gtttgccggt
     5101 tgaggcggcc gccgttccga gattggctgc agacgcacca gggcccggcg ccggccgctt
     5161 cgccacaatc gaaacgatga ttaccgaact gctccagaag ctgctccgcg gcgagcacct
     5221 gacgaccgac gaggccaaac aggcgatgaa cctcatcatg tgcggcgagg tcacgccggc
     5281 ccagatcggc ggttttgtca ttgccctgcg ccagaaaggg gagacgccgg aagaggttgc
     5341 gggctttgtc gaggccatgc gcagcctgtc gctccgcatc gaactggacc gcacccgcga
     5401 gacggcggtc gacggctgcg gcacgggcgg cgacggcgcc aacaccttca acatttccac
     5461 cgccgcctcg ctcgtcgttg cggccgccga catcccggtc gccaaacacg gcaaccgctc
     5521 ggtcagctcc aaatgcggct cggccgatct gctggccggt ctcggcgccg acatcgaccc
     5581 cggcccggag gtggtcaagg agaacatcga gcgggtcggg ttcggctttt tgtttgcccc
     5641 gcgctaccac ccggccatga gacacgccgc tctcccgcgc cgggagctgg gggtccgcac
     5701 gatcttcaac atgctcggtc ctatgaccaa cccggccggc gtcaaacgcc aggtcatcgg
     5761 cgtttacagc cgccaggtca tgtacctcat ggccgaggtg ctcgccctgc tcggctcccg
     5821 acacgttctg gtcgctcatt cccgcgacgg tctcgatgaa ttctcgatta ccgccccgac
     5881 cgactacatc gaggtacggg acggctggat tgcgaagggg acgctcaatt ccgaggatgt
     5941 cggtctgccc gagcgctacc ccggtgtgct tgagggcggg gatatcgccc ggaacgcggc
     6001 gattctcgaa gacgtgctcg cgggccggga atcggcctac cgcgatgcgg tggcgctcaa
     6061 cgccggggcg atgatttacg ttgctgacag ggccgagtcg atccgcgagg gcatcgtggt
     6121 cgcccagcgg gcgatcgatt cggggggggc aaaagatctg cttgaccgct ggcgggcggc
     6181 gacgcactct taatcaatga agtccgccga accgcagctt gatcctgcac cgccttcggc
     6241 ccgttcaggt ccgcccttcg tctccgggct gagagactac ctggaggtga tccgcctgtt
     6301 ttcgcggaac gcctggctct ttttgatcgg gtcgtttctc atggggatca atttccaggt
     6361 cttcaacctc ctgctgaatc tgtacttgaa ggagcttggg tacgccgagg gggatatcgg
     6421 cctggtggcc tccgggcggg cgatcggcat gaccgccatg gctattccgg tggcggtggt
     6481 gctcagccgg gtccggctca aaccgattct cctggccacc gtcgccatct tcgctttctt
     6541 ctcggtgttt atcgtgaccc tggaccgcct gccgctgttg gtcggttcgg ccgttctctc
     6601 ggggatggcg tttgcgttct accgggtggc gggggggccc ttctacatgc gcaattccac
     6661 cgcccgggag cggacgcact tgttctcgct gtcgttcggg gtcatgattc tcgcgggcat
     6721 ggccggctcg ctcctggccg ggaagacggc cgccctcctc accgcgcgca ccggcgacct
     6781 catcctcgcc taccgcctca cgctggtcgg cggcatcggt ctgtcgctgc tggcgctcat
     6841 tccgttcgcc ttcattcagg ccgctgcgcc ctccgcggag gagaaccgcg tgattttcac
     6901 gcgggagcgg ttccgcagcc gcggcctgtt ctacttccag atcaccctgg tcaacctgct
     6961 gatcggcacc ggggccgggc tggtcatccc cttcctcaac ctgtatttca gcgaccgctt
     7021 ccagcagccc gccgacacca tcggctacta cttcttcctg gtcaacctgt cgatgctgat
     7081 cggctcgctg gccggaccgg tgctggccaa gcgccacggg atggtgcgca cggtggtggt
     7141 cacgcagttg gcctcgattc ccttcatgct ggtattgtcc tacgggttca tcctctggct
     7201 cgcggtggcg gcgttcgtga tccggggcgg acttatgaat ctcggctctc ccatcgtaac
     7261 aaatctggcg atggagctgt ccgaccgctc cgagcagggg ctcgtgaacg ccctcctcac
     7321 cgtctcgtgg accgggtcgt ggatggtttc gaccgcgctc ggggggcaga tgatcgagtc
     7381 gttcgggtac actgtcgcca tgaacatcac gatcatcctg tacgtgatct cctcgctggt
     7441 cttctatttc ttcttccgga ggaccgagaa gaaggtggat cgggtcaacg gctgggtgat
     7501 tatgcgcgga gagacgccgt gacggatctt ctgcagcgaa ttgtcgccga caagcgggcc
     7561 gaggtagagg cgctcaaagt tgcgcacccg ctggagagtt ttcgggacag cctcgatccc
     7621 gggtcggtcc gggacttccg cgccgccgtc tcggccgccg gccggaccaa tattatcgcc
     7681 gagatcaaac ggggctctcc cgccaagggc atgctgtcgc gggtgttcaa cccggtcgtg
     7741 ctggcggccc agtatcagca ggggggagcg gccgcgctgt cggttctcac cgagcgccgg
     7801 catttcttcg gccactacga gtttgtccgc ctggccggca agcgggccgg cctgccggtc
     7861 ttgtgcaagg acttcatttt cgaccagtac cagatctacc acgctcggtg gatcggcgcc
     7921 gatgcgatcc tcctgatcgc gcggctgctg gccccggacc agctggccga gttcatcagc
     7981 cgcgcccgcg cggtcggcct ggccgcgctc gtcgaggtgc acaaccgggc ggaggtccgg
     8041 gccgcactcg acagcggggc cgaactgctg ggtgtcaaca gccgcgacct gagcgatttc
     8101 acggtccgcc tggagacggc cgaggagctg gcgcctctca tcccggcggc ggtaatcaaa
     8161 atcgccgagt cgggtatacg cgcacgggag gacgtccggc ggctcgagcg ggcgggctac
     8221 cgggcgttcc tgatcggcga ggcgctggtg acttcctcca acccggcggc cctcctgcag
     8281 gagttgcgcg gcgtatgatt ccgtttcgcg tgaaagtgtg cggtataacc cgccccgagg
     8341 acgccgcggt ggcggtcacc aacggggccg acctgatcgg cctgattttc tactcccgct
     8401 ccccgcgccg ggtcagcccc gatgcggccg cggccattat cacgaagctc ccgccggtcg
     8461 tgcaccgcgt cggcgtgttt gtcgatgacc ctcttgaccg ggttctcgcc accgccgcgc
     8521 ggctcgatct cgatttcctt cagctccacg gaccgtatac cgatgacgac gttcgcaggg
     8581 cgcggcgcgt gggtttccgg acgatccgcc tgcgcacggt ccggcgcccg tccgactacg
     8641 cggccttcta caccgacccg gccgacatcg tcatggtcga ccacccgtct ggcgcccttg
     8701 ccggcggcac cgggaccgct ttcgactggc gcatccgccc gcccgtgccg attgtcaatc
     8761 tcatgctggc cggaggcatc acggccgaca acctggccga gggagtgcgg acgttccaac
     8821 cgctcgtggt ggatgtgaac tccggggtcg aggaggcgcc cggcgtcaaa tcggcggaga
     8881 agatgaaacg attctttgag gtcgccaacc ggctacgcta tgccaaggcg gactaggcga
     8941 cagcccgatc ggcgcgggcg gtttggcccc tacggcggcc gctatgtccc cgaaaccgtc
     9001 atggccgcgc tcgatgacct cgagcgggcc taccggggga ttgcgcgcac gccggagttt
     9061 cggcgcgagc tgggcgggca cctccgcgat tttgtcgggc ggccgacccc gctgtatttt
     9121 gcggaacggt tctccgagcg ggtcggccgt gcggtttatt tcaagcgcga agacctcaat
     9181 cacacgggcg cccacaagat caacaacgtg ctcgggcaga tcctcctggc ccggcgcatg
     9241 ggcaagcggc gagtgatcgc cgaaaccggc gccggccagc acggcctggc gaccgccgcc
     9301 gcgtgcgctc gtttcggcct gacctgcatc gtctacatgg gggccgagga tatcgtccgc
     9361 cagaaaccca acgtcgagaa aatggctctc ctcggggcgg ccgtcgtccc ggtggagagc
     9421 ggctcccgga ccctgaagga cgccacgagc gaggccctgc gcgactgggt caccaacgtg
     9481 cgcgacacgt tttatgtcat cggctcagtc gtcggcccgc acccgtaccc gatgatcgtg
     9541 cgcgatttcc agtcggtcat cgggcgcgag accctcgccc agtgccgccg gagatttggg
     9601 gcccttcccg acgccctcgt ggcctgcgtc ggcggcggct cgaacgcgat cggcctcttc
     9661 gccccgtttc tgcgccacga cgctgttcgc cttttcggtg tggaggcggc cggcctcggg
     9721 ctgcgttccg ggcgccacgc cgccaccctc acccgcggaa aacccggcgt gctgcacggg
     9781 acctacagtt acctcctgta cgaccgagac ggccaggtcg ttctgccgca ttcggtatcg
     9841 gccggactgg actaccccgg tgtcggtccg gagcatgcct tcctcaagga tgcgggccgg
     9901 gtggagtatg ttgcggtgac cgatcgcgag gctctcgccg cctttcgcct ggtgagccgt
     9961 ctggaaggca tcatcccggc cctggagacg gctcatgcgc tggcctggat ggtcaaagcg
    10021 gcccgccggt tcggaaaatc cgaccggatc gtggtcaacc tctccggccg gggcgacaag
    10081 gacatggcga ttgtggggaa agcggggaag accttccgat gagtcgcgtg tcccgcctgt
    10141 tgtcggcccg acggcccggg cacaaactgc tggcgccgtt tttcaccgcc ggctttcccg
    10201 acccggcggt gtggcgggcg ctggtcggca cggccgccgc ggcgggtgcg gacataatcg
    10261 agatcggcat acccttctcc gacccgctgg ccgacggccc ggcgatccag ttctcctctc
    10321 attgcgccct ccggaacggc ctcagcctcg ccgttttgct cgacggcatc gccgacctgc
    10381 gggcgcggct ggatctcccg ttcatcctca tgggctacta caatcccctc ctcgcgtacg
    10441 gcgaggagcg gtttctggag gatgcggccg cggccggggt cgatggcctg atcattcccg
    10501 atcttccaat cgaagaagcg gtgtcctacc gccgagcggc gcggcggcag ggtctgtcga
    10561 ccgtcttttt ggtcgccccc acctcgactc ccgaacgcgt aaaactgatt gacaaatcat
    10621 cgaccgattt tgtatatgct gtgaccgtga ccggggtcac cggcacgggg aagacctttg
    10681 gcgaagcgac cgacgcgtat ctgcggcggc tgacggcggt tctgacccgg ccgttcgtcg
    10741 ccgggttcgg cgtcaacggc ccggccgccg cgcggcggat ggcgcgctat gccgacggcg
    10801 tcgtcatcgg ctcggccctg gtcgaagtga tccgctcggc cgccacgaaa cggcaagcgg
    10861 cgaccgaggt gggccggttt ctggacaccg tgaggcgcgt tctcccatga cggcgaaagg
    10921 tatcgacaga ctgcgcgatc agattgacgc cctcgaccgg cggcttctca gcctgctcaa
    10981 cgagcgggcc gggctggtgc tcgatttggc ccggctcaag cgggccgaca ggctgccggt
    11041 gtttgacccg gcccgagaga aacagatcct cgaacaggtc gtcggtcaaa accgcggccc
    11101 cctgcccggc gacgcagtcg cacggctgtt tgaacggatt atcgatgaat cccgccgcct
    11161 cgaacgcacc gaagtgtacg acacgccgtc ggaatgaaag agaccccatg atcgtcatca
    11221 tgcaggagca ggccagcgac gcacagatcc agcaggtgat cgcgaaactc atcgacctgg
    11281 ggttcgacgt ccatcgctcc acgggcgccc tccagacggt gctcggggtg gtcggcaaca
    11341 cccgggcgct cgacgcccgc aatttcgaga ttctcgaggg cgtgcaggag gtcatccgca
    11401 tcaccgagcc ctacaagctc accggccgca ctttccgccg gcacaacacg gtggtgcgga
    11461 cgggggaggc ggcgatcggc ggcgagcggg tcgctctcat cgccggcccc tgctcggtcg
    11521 agagccggga gcagatcagg aaaatcgccg cctttgtgcg gtcgaccggc acgctcctgc
    11581 tgcggggcgg ggcgtacaag ccgcgcagtt ccccatactc gttccagggg ctcggcgagg
    11641 aggggctcaa gtatctgcgc gaggcggccg acgagaacgg gctgcgcgtg gtttccgagg
    11701 tgatggaccg cctcgatatc atcccggccc tcaagtatgt cgacatcatc caggtcggtg
    11761 cccgcaacat gcagaatttc gcgctcctga aggagctggg gaaaatcgat aaaccgatct
    11821 tgctaaagcg cggcatgtcg gctaccttga aggagttatt gatggccgcc gagtacatta
    11881 tggccggcgg caaccataac gtggttttgt gcgagcgcgg tatccgcacc tttgagagcg
    11941 ccacgcgcaa cacgctcgac atttcggcga tcccggtgct gaaaaagctg acccaccttc
    12001 cggtcatcgt cgatccgtcg cacggcaccg ggttgcgcga caaggtggcg ccgatggcgc
    12061 gcgccgcggt ggcggcgggc gccgacggcc tcatgatcga ggtccactat gaccccgacc
    12121 aggcgctctc cgacggcgcg cagacgctct ttttcgagca gtatgcgcaa ctggtgacag
    12181 aaatcggcat cattgcctcg gcggtcggcc gcagcctctg aggcgccggg accgtccccc
    12241 ggcggcagcg cggagcgtag ggaaagcgat atcggatgtg atggccagag acaagtacaa
    12301 ggcgcagttc gcccatgccg tcgccaaggc gtttcggcag gtctatcccg aggtctaccg
    12361 cgaggccggg ccggcgcagg tgttcgatgc cgacttcgtc tacgacaacc tcgaaaagcc
    12421 caaagacccg gccatggggc gctttgctct cccggtcttc cgcttcggca aactcctccg
    12481 cgacgccccg ccggccatcg ccgccaaagt cgccgcggcc tcgcccgcca acggcggcaa
    12541 agacatgctc gtccgggtgg aagccgccgg cgggttcctt aacgcccgca tcgacgccac
    12601 ggcgctcgct ttcgaaacca tcgacgacat cctcaccgag gcgaaccact acggcggttc
    12661 gcaggcgggc aaggggaaga ccgagctcgt cgagtattcc tcggtcaaca tcgccaaacc
    12721 cttcggtatc ggccacctcc gcaccaccat cctgggcaac tccctccgcc ggatcttcgc
    12781 caagctcggc taccggacgg tggggatgaa ttacctcggc gactggggca cgcagtttgg
    12841 caagatgatc gtcgcctacc ggcagtgggg cggcggtctc gacctcagcg acaacgcggt
    12901 cgagaaactg gtcaagctgt acgtccgctt ccaccaggag gcggaggggg acgaggcgct
    12961 caacgagcag gcccgcaagg cgttcaagga tctcgaagac ggtgaaccgt ccgcggtcgc
    13021 tctctggcag cagttccgcg acctctccat cgccgagttc atgcgcgtct accgccgcct
    13081 cgggatcgaa ttcgacgtga tcaccggcga ggcgtacctg aacaaacgga tggggccgat
    13141 gatcgagcgg ctggtccgca ccgggctggc gtccgagtcg cagggggcgt tgattgtcga
    13201 tctaggcgac ccgcagttgc cgccccttct gttgcgcaaa gccgacggcg ccacgctcta
    13261 ctccacccgc gatctggccg gcgccttctg gcgctgggaa agctaccggt tccacgagtg
    13321 cctctatgtc gtggccaccc agcaggccga tcacttccgg caggtcttcc ggacggtcgc
    13381 gctggtcgag gaggccgaga atgtcccgcc cgaggaccgt ttcgccggcc gtctcaagca
    13441 catcgatttc ggctgggtgc ggttcggcga cgccaatctc tcgacccgcg ccggcaacat
    13501 catcttcctc gaagatgtca tcgacaaggc ggtcgccctg gcgcgcgaga aaatcgccga
    13561 gaagaatccc ggcctcgtgg ccatcgaaga caccgccgaa atgatcggca tcggcgccgt
    13621 gatgttttcc cagatgtcgg tgcggcgtca gaaagacgtc aacttcgact gggatgaagt
    13681 gctcagtttc gagggggaga ccggaccgta tctccagtac acgcacgccc ggctctgctc
    13741 gttgctgcgc aactacgggc gcgaggtgtc gccctcgttc gacccggttc tgcttgagcg
    13801 caacgaggag cagcgggtga ttgaagcgct cgccgatttt ccggaggcgg tcgccgacgc
    13861 cgcgcgcgcc tacgatccgt tttacgtggc cgcccatttg atccggctgg ccggggcgtt
    13921 caacaagctc taccagcgca agaacgagga cggccgcatc gacaagatca tttccgacga
    13981 cccggccctc accgaggccc gcattgcgct ggtcaaggcc gtccagattg tcataaacga
    14041 aggcctccgc ctgctgggga tccgcgcccc ggaggagatg tagccgtggc ggcgtcccgg
    14101 gggccggtga gaaaactcgc catttgcggc tgcggcctga tcggcggcag cctggcacta
    14161 gatgtgcagg cgcgggggcg cggcgtggag atcgcggtct gggatcggcc gggcgtcctc
    14221 gggcgcatcg ccgccgaccg ccgtctccgc gttcgccccg aggccagttt ccgccacgct
    14281 gtcgccggcg ccgacgtcat tgttttggcc gcgcccccga ctgcgaacgg gacgcttctc
    14341 cggcgcctgg cggcccggcc cgaggtggcc ggcgccctca tcctggacac cggctcggtc
    14401 aagaagccga tcgccgacct ggccggctcg ctcacgtttc cgccggggac gcagtttttg
    14461 ccgtcgcacc cgatggccgg acgggagaaa tcggggtcgg cggccgcggc ccccggtcta
    14521 tttgcgggcc gcccgtggtt tatcgacgaa accgtccgcc tgtccgtctc acaccgggcc
    14581 cgctggaact ggctgcgggc cgccgttggc gcgaccccgg tgctcatcga cagcgccctg
    14641 cacgatgaac tgatggccga gctgtcccat ttgccgcagc tgctggcgac cgttctcggt
    14701 gcccagaccg atccccgcac ggtcgaactg gcgggacccg gactggcctc cgtgctccgg
    14761 ctggccggtt ccccgcacgc tgtctgggcg gagatcatcg aggccaaccg agctccgatc
    14821 ctcgccgcgc tgcgcctgta ccgggacaac ctgaacaccg tcatggaccg gatcgcccgg
    14881 ggcgaatcgt tgcgcgacgt tttcgccgcg gccgccagga gttatcgatg cttgtcgtga
    14941 tggagatatc cgcctcgcgc gaggccgtgg agaatgtctg ccgcctgatc ggggagatgg
    15001 ggctgaccgc ccacgcgatg ccgggcgccc agcgcacggc gatcggcgtg accggcaaca
    15061 agtcgcagat cgacgccgac cgcatcctca cccagccggg cgtcaaagag atcatccacg
    15121 tcaccaagcc ctacaagctg gtgagccggg agtttatccc agaagatacg gtggtcgacg
    15181 tcggcggcgt gccggtcggc ggcccggagt ttgccgtcat ggccggaccc tgttcggtcg
    15241 agagccgcga gcagacgctg gcggtggccg aggcggtctc ccggctggga gcccggatcc
    15301 tgcggggcgg cgcctacaaa ccccgcacct cgccctattc gttccagggg ctgaaggaac
    15361 ccggcctcaa gatcctcgcc gaggcgcgcg agcgctacgg tctgaagatc gtcaccgagg
    15421 ccatggacac cgatacgatc gacctggtgg ccgactacgc cgacatcatc caaatcggcg
    15481 cccgcaacat gcagaactac tcgctcctgc ggcgggccgg gcagaagcgc aaacccgtcc
    15541 tgctcaagcg ggggcttgcg gcgacggtcg atgaactgct catgtccgcg gagtacatca
    15601 tggccgaggg caaccgccag gtgattctct gcgagcgtgg cgtgcgcacg ttcgccgacc
    15661 acacccgcaa cactctcgac ctgtcggcca ttccctatgt caagcgggcg agccacctgc
    15721 cgatcctcgc cgacccgagc cacggaaccg gccgcaagga caaggttctc ccgctctcgc
    15781 gggcctcgct ggcggtgggg gcggacggcc tgctgatcga ggtgcactgc gatcccgcca
    15841 aggccctctc ggacggtccg caggcgctga cgcccgacct gttcgcgcag ctgatgaaag
    15901 aacttcgtct catggcgccg gtactcggca ggacgatggc atgaggacgt tgctgccggg
    15961 ccggatcgcc gacaccgtcc tccgcgtgcc gggggacaag tcgatcggcc accgggccgc
    16021 ggtgctggca ctgctggccg agggtccgct caccatccgc aacttcccgc attccgccga
    16081 ctgccacgcc tccctcgagg cggttcgggc gctgggggcg acggttgccg atgacggcct
    16141 ggagctggtt ttgaccccgc cgccggaact gcgcgcggat cctgacacgg tcatcaattg
    16201 cggaaactcc ggcacgactg cccgcctcct ggccgggctt ctcgccggct cgtgcgtcga
    16261 agtgacgctc agcggcgatg agtcgctgcg ccgccgcccg atggcgcgga ttatcgaccc
    16321 gctggcggcg atgggggcga cgatcgccgc ctccgacggt catctcccca tgcgtgtccg
    16381 gggcggcaga ctgctgcctt tcgagtatac gctcccggtc gcctcggcgc aggtgaagtc
    16441 ggcgctcctt ttggcggggc tggcctcccg ctgcgccgtc accatccgcg agaccgcgat
    16501 cacccgcgac cacaccgaga ttttgctggc cgaactaggg gaggggctcg cggtgcggaa
    16561 agtcagaccg gtgctggttg ccgacccgca cgatccccgc aagcgccggc aggaaatgcc
    16621 ggagccgttc aaacgggaaa tcacgcttgc ctcgcgggcg gtggtccgcg gcggcacagt
    16681 cgacattccc ggcgacatct cgaccgccgc cttctttttc gccgccgccg ccatatccgg
    16741 caagaccatc accgtcgagc gcgtcggcct taacccgacc cgcaccggga tcctcgagca
    16801 cctccggggc cttggctgcc gggtcgacat cagcgagcgc gaaacggtca cgggtgagcc
    16861 gcgcggcacg gttgccgtca ccggcgcccc gctcggggcg cgccgcatct ccggcgaaac
    16921 caccgtcgaa ctggtcgacg aaatcccagt catcgccgtc atggccgcat ttgcggaggg
    16981 gacgaccgtg atccgcgacg ccggcgagct gcgggtcaag gagagtgacc gcctggctgc
    17041 ggtcgccgac aacctcaagc gcatgggcgc caaatgcgga ctactcgaag acggcctggc
    17101 gatcgagggc ggcggacagc tcgaaggggc cgacttccgc acctacggcg accaccgtat
    17161 tgccatggcc ttcgccgtcg cctcgctctt cctcaccggc ccgtccacgc tcgacgacga
    17221 tgaatgcgtc ggcgtctcct gcccgacgtt cttcgacctc ctcaaccaga tacgggcatg
    17281 accggccggc tctcgtttgc gctgatcggg gagcacatcg cgtactcccg ttcgccggcc
    17341 gtattcgagg ccatcttccg ggccgatgga gtcgaaggct cgttccgggt gcacgaggtc
    17401 tccgccgcgg cgttcgccga ggagttcccg cggctcctgc ggtccggggt gcacggtctg
    17461 gccgtcacca tcccgcacaa ggagcgggtc gcgcctttcc ttgacgacct cgatccggtt
    17521 gctcgggccc ttggcgccgt caattccatc gccatcgcgg accgtctgcg cggtttcaac
    17581 accgatgtcg ccggtttcag atttgccttg gagaattacc gggcggtggt tgcgggacga
    17641 ccggccctcg tgctcggctg cgggggggcg gcccgggcgg cggtgtacgc cctccaccgc
    17701 cacttcggag taccgtccat cgtgatcacc tcgcgccgcg cggagacgct ggagaggacg
    17761 gttgagcggc tccgcaaggg catggcgggc ctggaaatca cgccggcgcc ggcggcgcgc
    17821 ctgctgccgc tcgaccgtcc cgtgggagta gtggtcaacg ccactcctct gggcggcgga
    17881 aaccatcccc gcgaactgcc gctgccgaaa gcgtttgact ggacgcgcgc ggcggtgtac
    17941 tatgacctca actataacga tgacaatcag gcggtcggct gcgcgcgcga ggccggcgtg
    18001 gcggctctcg atggcgccgc gatgctggtc gcccaggcaa ttgaatcata ccggcgctgg
    18061 accggccgaa cggtcccgtt cgatccggtg ttcgccgccg ccttcccgcg gcggacgcgc
    18121 tgaattcgag ccccgagagc gatgaagaag gcgaaggacc ccgagaagat ttttctggtc
    18181 ggtttttccg gaagcgggaa atcgacaatc ggcccgcttt tggccgcgcg cctcgggcgt
    18241 ttctatgtcg ataccgaccg ggtgatcgcg gagaagttcg gcaaggacat ccccgggata
    18301 ttcgccgacg acggggagga ggcgttccgg gccgccgaga ccgaggttct cggcgagatc
    18361 gcgggccgcg cgggcgtgcg cgaggtggtc gtgctgggcg ggggcgcttt caccatcgcc
    18421 gtcaaccgcg acctggttcg ccgcgccgga atcgccgtct acatccagtg ttccgttcgc
    18481 gagctcgccc gccgcatgcg ccgccacgcc gaccgcccgc tgctgacggt cgtccccaac
    18541 cccggcgaat ccctccccga ggcgacccgc cgacgggtgg ccgaactgct cgaggagcgc
    18601 cgcgagttct accttttggc cgacatgacc gtgtcgtcca ccgcccgcct gccggtcagc
    18661 gtggtcgaag agatcgtgcg caagctggag aaccgctatg agccgagttg atgtctgttt
    18721 gggggatcgc cgctacccgg tgctcatcgg ggaaggcggc cgggagcgcg atttgccgcg
    18781 ccttgtgcac cgccaggcgc ggcggggcgg gcgggtcttt gtcattttcg atgcccaggt
    18841 ctgcgctctc tacggcccgg aacttgttgc acgtctcgcc gacagcggcg gttcgatcca
    18901 ccagatggtg cttccctccg gcgaacgttt caagactctc tcggtcgcgg ccgccgttta
    18961 cgacttcctc ctgcgccgtc gggtcgcccg cgacgatttc ctgctggcgg tcggcggcgg
    19021 agtcacgacc gacctggcgg gttacacggc cgccactatc ctgcggggga tcccgtgggg
    19081 agcggtcccg acgacgctcc tgggcatggt cgacgccgcc atcggcggca agaccggcgt
    19141 caatcacccg ctgggaaaga atctgatcgg cgcgttctgg cagccccggt tcgtgctctg
    19201 cgacgtggga tttctgccca ccttgcccga ccgcgaactg gtcgccggtc tcggcgaggt
    19261 tgtaaagtac gcggggttgg cgggggagac gttcctgcgc ctcctggagt cgtatctgga
    19321 tggcgggaat cttctggagg cgcgctccct cagtcgcctg gtgcgtctgt cggtggcgta
    19381 caaggccggc gtggtcgtcc gcgacgaacg cgagtccggc ccccgcatgg cgctgaatct
    19441 tggacacaca ttcggccacg ccgtggaaca ggcggccggg ttcgggcggc tgcgccatgg
    19501 cgaggccgtg ctgctggggc tcgatgccgc agtcgagctg agcatcgcgc agaaaccgtc
    19561 gcgccgggcc gggctcgccg gctaccgggc gctggttcgc cgcctgctcg gggtgcttcc
    19621 gcgtcgccgc atatcggccg aggcggtcat ggaagcgctc gcgctggaca agaaacgcgc
    19681 cggaacgacg gcccgatttg tcttgctcga cgccccgggc cggccgttaa ttgggaccga
    19741 ggtcccggcc gcggcggtgc gcgacgcggt gagggcaatg ctcgagcact atgcgccgga
    19801 aggaggccgc catgtccaga gtcctcgtcg ttaacgggcc caatctcaat ctgctcggtc
    19861 ggcgcgaacc ggagatttac ggcaccgcca ccctggagga tctccacacc cggctccgcg
    19921 aattcgccgc ggagatgaag atggagctgg cgttcttcca gtccaacgcc gagggcgaaa
    19981 tcatcgagtt catccagcgg gagggcccgg gcgccgacgg cctggtaatc aacccgggcg
    20041 ccctggcgca ctacagccac gctctgcgcg acgctatcac ggccgtggga atcgagaccg
    20101 tcgaggtcca tctcacgaac atctacgccc gcgaggaatt tcggcgccac tccgtgatcg
    20161 cgccggccgc gcgcggccag gtggtcggat tcggcatgta cgggtacgct atggccttgt
    20221 cgtattttgc cgatacttcg gataaatgac cgatccccgg attggcgcac catgaaacgg
    20281 cttgtgttac tgctcatagg cctcagcctg cttgcggcgg cctgccggga acagcctgtc
    20341 tcgcgagacg acgtgttctt ggtgatggac tccctcgagc acaagttcga ctggctcggc
    20401 taccgcctcc agcaggagac ctgggagatc tacagcacgg ggcagtccga ctccctggat
    20461 ttctaccagg gactcctccg ctacacggtc gccgatccga atctgctgcg caccttccgc
    20521 cagggccgcc tgtgggtgac ggagaacgag gacgtccggc gcctcgactt gctggaagcg
    20581 gcgctcatgc gggcccaggt cgaggtggcc gagccggtgg cctccctcca cgactcgctc
    20641 gtgcagatcg atgtcacttt ccgagcgccc ttcgacggcc agaaccggac tcaaaattac
    20701 ctgtaccgta cgtatcgatc cgaccccgac cgcagccgcc gcgaggcggc ctttcgcgcc
    20761 taccaccgca tcggcgatct gctggccgac gggctggccc ggctgatccg gctgcgcaac
    20821 cgcgaggcgg ccaagttcgg acagcagaac tatttcagcc ttctcttttc gcagcagggg
    20881 ttcgacgagg cccgctacct tgccaccctg gaccagcttg actcgctgag tctcgagccc
    20941 taccgccgga tactggcgaa cgcgggggag aagctggggt ccgggccggt ggagatctgg
    21001 gacctctcct acgcgtatgc cgatatcaac cggcggggcg acgcctactt tccggccgac
    21061 cggcagttca gccagattgc gcgcagcctg gaggcgctgg gctacgacct cgacaaactg
    21121 ccgatctatt tcgaccttca gccgcgcagc gacaagacgc agctcgccta cgccgtcccg
    21181 ctccggccgc cccacgacgt ccgcgtgctg gcgaatgtga ccgacggcta cctgagcgcg
    21241 cgcacgctca tgcacgaaat cgggcatgcc cttcactttg cccacatccg gcaggagcgg
    21301 cagctttttg tcgactgcgt gagcgaggcc tggactgagg ggatggcgca gattttcgcc
    21361 tcgctgctcg acgatccgca gtggctggtc cagtatgcgg ggatgacgcc ggagacggcc
    21421 gccgagtttc tgcgcgcccg ggccgaacag gagatcattt acctgcgcac caccctcatg
    21481 cgtctgcgct gggaatatga ggcctatcgc aacccggatc aggatttcaa cacgctctat
    21541 tgggatctct tcgcccggta tctcctgctg ccgcgccacg acgacatccg accgtgggcc
    21601 acgattatcc agtatacgac ccacccggtc tatctgcaca actacctgta tgcggacatg
    21661 atcgcgtcgc agacgctggc gtcgctgcac accgtctacg gtccgctcat cgacaatccc
    21721 tcgctccgct ccttcctcaa tcaaaactac ttccgcttcg gtgcgcgcta tgactggcgc
    21781 gacctgctcg agcgcggcac cggtcagcgc ctcgaccaga attatctgat ccgccgactc
    21841 tgcatcgagc ctccggccgc cgccttttag gcggcgcgcg gccgtcccgg gagcggcccc
    21901 ctgaattgcg cttgttcccc gtcctgaaac accctaccat taccgctctg accattgcag
    21961 gagagcccgg tgacaagaac cgagacaatc accagcggac cgatcagcgg caccatcatc
    22021 tcgctcgcga ccccggtcgt gctggcgcag ttcatggaat tcgcgctggc cagcaccgac
    22081 tacttctggg taggcaggct cggcccgttt gcccaggatg ccctcaccac gtccatggtc
    22141 acggtgtgga ccatgttctc ggcggtttcg gtgatctcgg taggcgtcgc cgccttggtc
    22201 gcgcggcaca tcggcgccgg caaccccggc caggcggcgt tcttcgcccg gcaggggatg
    22261 gggatgggcg tcctgttcgc gatcgccgcc gctctggcgg cctgggtgct cactcccccg
    22321 tttctccgtt tcatgcaggc cggtccgcag acgctcccga tggctcagac ctacatgcgc
    22381 accgtctatc tcttcgccct ctgtttcttc ctgctggagg tctcgtacgc cgtcttccgg
    22441 gccgccgggg acaccaggac cccgaccatc atcgccgcca tcaccatcgt gctgaacatc
    22501 ggcctggacc cgctgctcat attcggctgg ggcccggtcc cggccatggg tgtggcgggg
    22561 gccgcgctgg ccaccggcct gtcggtcacg atcgcggcgg cgctggcgct cacccgcctg
    22621 gccggcgggc acctcggctt ccggctcggc cccgtgcggg cttttcgccc cgacttccgc
    22681 cagatgctcc gcatcgcccg gatcggcctg cccatcgcca gccagcagtt cattttcgtg
    22741 gccgtgtact ggttcctcat caaatacgtg cacgcgttcg gcccgaccgc gggcgcggcc
    22801 atggggatcg gcaaccgcat ggaatcgttc tcgtatctca tctgctccgg tttcgcggtg
    22861 gccgcctcga cgatggtggg gcagaacctg ggcgccggga aacccgaccg ggccgcccgc
    22921 tgtgcctggg gcgccaccgg catcgccgtc gccgtgactc tcgcggtggc ggtggtcttt
    22981 gtcagcctcc cccgcgccat cgcttccgtc ttcaccgacg acccggaagt gctccggatg
    23041 gcggccgatt acttggtcat cctcggtctc tcgcagttca ccatggccgt cgaaatcgtc
    23101 ctcgaaggag ccttcagcgg gtcgggcgac accctcccac ccatgctggt gtcggtaccg
    23161 gggtcgctgg cccggattcc gctggcttat ctgctggcgt tcaccctcgg ctgggggatc
    23221 aacggcgtgt ggtggaccct gaccatcacc accacggtca aggcgctggt cctggcttac
    23281 tggttcagcc gggggcgctg gaagcgcaaa gaggtttgat tctatttgac cctgcccgac
    23341 cggccgcctt tattccggga aagcaaatgc ccgtccccga aactggagag aaccggtatg
    23401 ccccgtcgct atgcgcgtgt ccgcctgcca gaccggcggg ttgtgtgggc ccgcctgacc
    23461 gatgacgata tggtcgcgcc gctggcgcaa gccccctggc ggggggaggc ccccgcgggg
    23521 ccggcagttc cgcttgccca ggcgaccctg ctggctccga ccgaaccgac caagatcatc
    23581 ggcatcggtc tgaattacca cgcgcacgtc caggccagtt tctcagccga caaggcgccc
    23641 gaccaccccc tgatcttttt gaagccgccc tcgtcggtga tcgggccggg ggagaagatc
    23701 atccatccgc ctcagtcgca gcgcgtggac tacgaggccg agctgggggt cattatcggc
    23761 aaggaggccc gccgcgtgcc ggccgaccgc gccgccgact acatcttcgg gctcacctgc
    23821 gtgaacgacg tcaccgcccg cgacctgcag aagaccgacg gccagtggtc gcgcgccaag
    23881 gggttcgaca ccttctgccc ggtcggtccc tgggccgtct gcggcctcga ttactccgat
    23941 ctcctggtgg agggaatcct gaacggcgag atcatgcagt cggggcggac gagccggatg
    24001 attttcaaaa tcccggagct catcagctac gtctcctcgg tcatgacgct gctgcccggc
    24061 gacatcattt cgaccggcac gcccgccggg attgctccta tgaagccggg tgaccggatc
    24121 gaggtgcgtg tggagggcat cggctcgctg gtcaaccaga tggccgagta ggcgaggagg
    24181 ccgcggccgg gtacaaagga tgaggctgtt ggaggaacgc cacgcatgac ggagcgactc
    24241 tactataccg atccggacct cctgcggttt gaggcccgca ttctcgatgc cggccgcgag
    24301 ggcggccggt acttcgccgt cctcgaccga accgcgttct accccacttc cggcgggcag
    24361 ctcttcgata ccggcattct caacagcgtc tccatcgtcg atgtcaccga atcctccgac
    24421 ggaacgattc ggcactattc gccgcaggcg atcggagagg tcggggcggt cgtgagcgga
    24481 gcggtggacg ccgcccgccg caaacgccac cgccggcagc acaccgcgca gcacatcctg
    24541 agccacgctt ttctgcagct ccacggctgc gagacggtct ccgtgcacct cggcgaggag
    24601 tacggcgcca ttgagtttga caccggcgcg ctgtccgatg accagctcct cgcggtcgag
    24661 gaacgcgccg aggagattat cttcgccgac ctccccgtgg acatcctctt tgtgccgccg
    24721 gagcagatcg cgaccatccc gctgcgccgg ccgccgaaga aggaggggat tctccgcgtg
    24781 atccgcatcg gcgacatcga gtattccgcc tgcggcggga cgcactgtcg ccggacgggc
    24841 caggtcggcc tggtgaagat catcggggtg gacaaaatgc gcggccacgc cctcgtcaac
    24901 ttcctcgccg gcgaccaggc ccgcgacgac tacgcggccc ggctggaggt gaccgcccgc
    24961 ctgacccggc actttacctg caatgtccgg gatctcgggg acaaggtgct gaaactcgag
    25021 gcggagaagc gcggcctcca ccaggaggtg gttcgtttgc agagagaact gctgccgtcg
    25081 caggccgaca gcctcgccgg gcaggccacg gcggtggggg agaggctcgt cctggtggcc
    25141 gaactgccgc actacgatcc caagctggcg ctgcagctgg cggccctgct ggccgagcgg
    25201 atcaacggcc tggcggtgct cctggtcgaa agccggctct acattgcggt ggggccgggc
    25261 agcggcctgc atgcgggcaa tattgtgaaa gcgctgggcg ccgccaccgg tctgcgcggc
    25321 ggcggcgggc cgctccaggc gcagctcggc ggcgccgacc cggcccagct caccacctat
    25381 cgtgctcttg cggagaagct cgtgcgtgag gcgtaaccgg gccatcgtca ccgccatcct
    25441 cttcctgctg gccgccgccg gcgcggccgc ccagaaccgg cagggctttg tcctcgaacg
    25501 gagcaaccag ctcgaggtgg tctgggaaaa tgagcgctat gtcacgtatg tcgacggcga
    25561 ggtcaagttc cgcaccgaga ccggcgccgt ctactgcgac tccgccacct tcgtccgcgg
    25621 tgaatacgcg aaactccgcg gccgcgtgct catcgacgac caggcgtata tgctccgcgc
    25681 cgactcggtc tactacgatc tgacccggga gacggcgctc gctctgggtt cgcaggtgca
    25741 gttgtggtcc tatgccgact cgctctacgc ggccggcgtc cacgcctttt acgatcgccc
    25801 ggcgcagcat ttcgagatgc aggagcgccc caccgtgtac ctcggctacc ctgacaccgc
    25861 ggcgatggtc gaggtggtgg cgaacacggt caactacttc gctcccgcgc gccgcgccga
    25921 ggcggtcggg cgggtcgtca tcacctcgca ggatattgcg gccgccgccg agtgcgccgt
    25981 catgcttatg gatcggaaca ctctcgatct ctatgacggg cctacggcca agcgcggccg
    26041 ctccaccatg accggcggct tcatgtccat cgctttcggc gaggaccagc tccgcgaaat
    26101 cgacgtcatc gactccgccc ggggcgaatt catcgaaccg atcgacagcg ccgagaccga
    26161 cgtcgaccgc tccattctcg ccggctcccg catcaagatg ttcttcgacg agggggattt
    26221 gcggcaggtg ttggcctccg gccaggccta ctcctggtac tacccctcgc cgcgcggcac
    26281 ccgcgaattt cacgaaaaca cggtctccgg cgacaccatc cgcctctttg tgcgcgagga
    26341 gcgtctggag aaggtcgcgg tggtgggcgg cgccgccggc acctacatca ccggcacggt
    26401 gagcgattca tccgccgccg gcgccgacac ggccgctgca ccggaaacgg cggtctcggc
    26461 cgccgtgcgc gattcgaccg ccgcggcaac cgccgccacc cgcctcgata ccgtcgacta
    26521 ccgcgccgag cacatcgttt acagcctggt ggactccctg atcacgctgg atgtgcgcag
    26581 cgaggtcaaa tcgggggcga tggagctgcg cgcctacgac gtggacttcg acacccgccg
    26641 ccgcgtggtc gaggctttca gcgccgaaat cactcccgat gcggccgacc gcgactatta
    26701 cagcctcgcc gagcggctcc agcccgccgc catcccggtg attctcaagg acggcgccga
    26761 tgaacttcac ggcgactacc tcgagtactc catcgacacc gagaaggggc ggatcgtgca
    26821 gaccaagtcc gagttcgagc aggggtatta ctacgggggg aagctgttcc ggcagaagag
    26881 ggatgtgtac tacattgagg acggctacta cacgacgtgc ggggcggacg agccgcactt
    26941 ccatttctac tcgtcgcgca tgaagttaat gcaggacaac aagctcatcg cccgcccggt
    27001 ggttttctac ctcggccgca ttcccctcat ggccattccc tactacgtct tcccgctcaa
    27061 gaagggccgc cactccggtt ttctcccgtt cacgttcggg cgcttcgagc gcggggagcg
    27121 gtacgtcaac aacctcggct actactgggc cgcttccgag tactgggatt accggggggc
    27181 ggtcgactac cacgacctct cgcgcacgat cactttcaag agcgaagttc agttccggaa
    27241 acggtacttg ctcgacgggt acctgcgcgg ggagtatcgg cgccagaccc agtacaaccg
    27301 gcgcgtcgca ctggagaact cgccgacgag ctattccttc agcggggcct acaaccacac
    27361 tctctcgccg tcattcgacg tccgctcctc cggcagctac gtctcgaccg ccagctacta
    27421 caccgactac tcgaattcgc tcgccgaacg gctcaaccgg aaccttgtct cgcaggtgaa
    27481 tttctccaag aagttcggcc gactgacctc ggtcaccggc agcgtgcagc acactgagaa
    27541 cctcgacctc gaacagcgca ccgaccagtt gccgagcctg tcggtgtcgc tgcccccgat
    27601 ttacccgttc ggcggttcgt cgacgaacga cgaaggggag accgtcaccc ggtggtacaa
    27661 ccagttcatc ctgcgctaca acccgaccct gctgaactac tcgacccgca gcaccatcct
    27721 ggccgacacg atccacacgc tcgtcgacac cctcatcgac acgaccttcg tcgacgatac
    27781 gacgatagtg gcgatcgaca ccacctaccg ggcggtggag atcgactccc tcatgtcccg
    27841 ttcgcggcgg cactacctga aagtgcagca caatccgagc atcacccttc cgaccattcg
    27901 gatcatcccc tacgtgccga tcgtcccgag cttctcgtac agcgaaacct ggttcaagat
    27961 cttcgagacc gaccagtcgc aggcggccgg gatcgacgcc tctgaattct accgcaccta
    28021 ctcctactcg gcgggcgtga gcgccaacac caagctctac gggacggttt acccgggcct
    28081 gttcggtctg ctcggcctgc gccatgtcct ggagccctcg gtcggctacc gctggtcgcc
    28141 cgacatacag cgctacccgg aggtgcgcag tttcgccggc gggggcgcgg ccagttcgaa
    28201 gagccagtcg atcggcttcg gcctcggcca tctcttccag gccaaggtga gaaccggcga
    28261 gaccgagaag aatatcgacc tgctctcggt ccggtcgtcg ttcgcctacg acctcgagcg
    28321 cgatttccgg ccgctgtcgc agatgacgac cagcttccag accagcacta tcccgcggct
    28381 caacatcagc ggcgacatgc gccacacctt ctacaatccg tacgatgact ccgcgaccac
    28441 gccgaagctt ctgtcgccgt ggctgctcga cttcgacctc aacctgtcct tcgatgtgat
    28501 ggggcggacg tttctgttca ccgatgaggc ggagccgggc gtcccgctcg gcgtcgattc
    28561 ggcctcggcg ctggccggcc ccgaactgcc catgccctcg gcggcccgcg ccggctggaa
    28621 cctcgccgtc acctactcgt tcagcgaatc ggggcgccac cgctcctaca gcaagtcgag
    28681 ctttgtcaac atcacgctcg gttttggcct gaccccctcg acctcggtca actacaacca
    28741 gcgctacgac ctggtcgagg gccggaccat ctccagccag gtgaacattg tccgcagcct
    28801 gcactgctgg accggctcgc tcttctgggt gccggtcggc tccaacaccg ggttcggctt
    28861 caaactctgg gtgaacgagc tccccgacat caagctcgac tccaaccacg acagtttcat
    28921 atccaccgac gtgctgcgcc gctaggggcc cggaaaagca ctgcggcgcg ctccgccggc
    28981 gggttttttg ttgacaaacc gagtcgacct ccaatcttta tgcgcgcaat gtcggatacc
    29041 gcaactacca aactcccaag gagggtcagg caatgaccaa ggacgagatg attgcccaca
    29101 tcgcggaggc ctcgggcatc accaagcgcc aggcgacctc ggctctggat gcgttcatgg
    29161 acggtgtgac caggcaattg aagaagaacg agaagatcag cttcgccggg ttcggcacgt
    29221 tcgccgtgtc ccagcggaag gcgcgcaccg gccgcaaccc gcagacgggc gaggcgatca
    29281 aaatcccggc gacccgcgtg cccgtgttca aggccggcaa gaatctcaaa gaggcgatca
    29341 agaagaaata gcgtccgcga ccgagctttc ggaggcagga gtttgcgctt ctgccttttt
    29401 tttattgaat ttgtagttga aacctccggg ccggcgcccc accttggaat cctatggcga
    29461 agaagaaatc cgcgcgcagg agccggaaat ccggcggctt ctccgaacgc gcccggcagg
    29521 cgctcggagt cgtgttcatt ctcctggctc tcttgctgct catctcgctc gtctcgcacg
    29581 acagcctcga tgatctccgg gtgacgggcg acgccgacac ccacctggcc gtcttcgaca
    29641 tccagttccg caatcaggcc gggctgatcg gcgcctacct gtcgtttttg ctcatgttgc
    29701 tggccgggtg gctgtccttt ttcctcccgg tcggtctggg cgtggccgcc ctgcgcctgt
    29761 tcgctcccga gcgggccaag cgctggcggt ttaatgtcct gctgctcttc gccatctccg
    29821 tcctcggcac catgctcgtc aacattcacc gcctggccgc ccacacgctc gaggccgacc
    29881 ccgatgcggt cggcggctac gtgggggaga agctgaccct cctggcgatc aagctcatcg
    29941 gcgaactcgg ctcgtatgtc cttttgggcg gctccatcct gctgctcctg gtgctgtaca
    30001 cttcgatcac ccccgttttg gccaaggctc tgcccttgcc ggggggggag acggtgcgga
    30061 aaatctacac cgtcatgcgc aacgccgccg tgacgatctt ctcgttccgc tggctcgtgt
    30121 cccagtcgga ggatgaagag gacgaggccg cagcggggga gagcgagcct gccgctccgg
    30181 cgccttcccg cgagcgtccc cctgacagcg cggagttcga ggacctcctc gacgagcaga
    30241 tcgaggccga tgccgtcccg gacggggctg atcccgctgc tgaccgtgaa ccgcgtccga
    30301 aaaagccgcg cgcgaagccg gcggcgtcgc ccagcgatga gccggtgacc cgcggctcca
    30361 tcgactaccg ttttccgccg ctttcgtgct tggcccggga ccccaacccg gctaatgcgt
    30421 cggtcaccga agaggagctc aaggcgacct cgcgtatgct gaaagaaaca cttgagacat
    30481 ttggcgtaag tgtatccggg gaaatagaat actatcctgg tccggtgatt acgaggttcg
    30541 agctcaaacc ggccgccggg gtgaaggtca accagatcgt caacctgtcc gatgacttgg
    30601 cgcttgccct gaaagccaag cgcatccgta tcatcgcacc catccccggc aaagcggcgg
    30661 tggggatcga aatccccaat cgcaaagcgc aactggtgta cctgcgcgac attcttgaag
    30721 acgacatttt cgcccggacg accatgcggc tgccgctggc gctcggcaag accacggccg
    30781 gcaagcccta cgccgttgat ctggccaagc tcccccacct cctcatcgcc ggggccaccg
    30841 gttcgggcaa gtcggtgtgc gcgaatgtcc tgatcacctc gctgatctac aagctccacc
    30901 cgcaccagat ccggttcatc atgatcgatc cgaagatgct cgagttgacc gcctactcgg
    30961 gcattccgca cctcggccgc ccggtcgtaa ccaaccccaa gcgggccgag aaagtgctct
    31021 ccgatgccgt ggtcgagatg gagcagcgct accgccggct ggccgccgct tcggtccgca
    31081 acattgaaga tttcaaccgg aaacaggagg ccgatgacca gcgcctgccg tacatcgtga
    31141 ttctcgtcga tgaactggcc gacctcctga tgtcgtcgac ttcctcgcgc atggaactgc
    31201 tgatcacccg cctggcccag atggcgcggg cagtcggcat tcacctcgtt ctggccactc
    31261 agcgtccctc ggtcgacgtc atcaccggcc tcatcaaggc caacttcccg gcccgcctgg
    31321 ctttccaggt atcgtccaag gtcgattccc gcaccatcat cgacgccaac ggggcggaga
    31381 agctcctcgg caacggcgac atgctcttcc tgtatcccgg ccagcccgaa ccgatccgta
    31441 tccacggcgc ctacctcacc ggcgacgaga ccgaggcgat cgtcactttc atcaaggccc
    31501 aggaacttcc catgctcaag ctggagaaca tttcgcaggc cacgggcgag gcggccgatg
    31561 tcgaggaagt ggatgccggc gatccgcttt tccgcgaggc ctgcgaagtg gtcatccgcc
    31621 ataagcaggg gtcggtctcg ctgctgcagc gccgcctcgg catcggttac cagcgggccg
    31681 cccgtctcat cgacaagctc gaaaaggccg gcgtcgtctc gccgttcgac gggtccaagg
    31741 cccgcgaggt gctggtcgac aagagttacc tggagactct ggccgccacc gagggggcgc
    31801 cggtcgccga acaggaaaca aactaatccc ccgctcggtc gtataaatgg caatgactaa
    31861 agtgcttatc tctgtcgcgg tgctcgcctg ctccgcggcc gcgttcgcct ccgatgatcc
    31921 tttcgagggc gccaagcagg acctcgcgag agctgcctgc gtgcaggtcc agttcctctc
    31981 cgtgctcgag tcggacgttt tcgacgtcgt cgactccgtc cccggcacgg ccatcatcgc
    32041 cgccgacggc cgcttcgcgg tctcggtcgg ctccgaggaa tacctctatg acaacgagcg
    32101 cctctatacc tactccgcgc agaccgacca ggtgatcatc gagaagccct cgggcgacct
    32161 tttcgtcggc aacgaaatcg cgctcgtcac gcggctcgac cggtactacc ggacggcgcc
    32221 ggtcgctgcc ggacgggagt atcgcctgac gcggacggac acgaccggca cgcagctgcc
    32281 cgatgagctg acggttctgc tccggcccga ttcctccgcg atcgaagcgt ttctctatcg
    32341 cgacatcaac ggcgatctca accgggttgt tttgttgcgc cagaacctgt ccgaggcctg
    32401 cgccgacagc gcgttcattc ccgcctttcc ggagtccgcg gagcgcatca agttgtagcc
    32461 gcgccatgaa gttctacatt cacaagctcg gctgtccgaa aaacgacgtc gatgccgact
    32521 acatcgccgc ccgcctcatc gacgccggcc acgagccggt ctgcgatccc ctcgccgccg
    32581 actcgatcat tgtcaacacc tgcgggttca tcctcccggc caaggaggag tcgatcacgg
    32641 aaatcctgcg gctcgggcag ctcaagaagg aggggcggct tcgcgcgctg ttcgccagcg
    32701 gctgtctgtc gcagcgctac ggtgacgagc tgctgaaaga aataccggag ctggacggcg
    32761 cgttcgggca cggcgctttg gattcgctcg ccgccgcggt ggacggcgcc ccccggtttg
    32821 accgcgccgt gcgcgtcgaa ccgcgccgcc tggcctacct ggactggagg gaccgcttca
    32881 tctccgatcc gtacccctgg gcgtacctga aaatatccga cggctgcgac cggggctgca
    32941 ccttttgtgc catccccggc atgcgcggcc gctttcgcag ccggccgctc gagtcgattc
    33001 tgcgcgaggc ccggtttctg gccgaccgcg gcaaacgcga actgatcctc gtctcgcagg
    33061 aagcgaccct ctgggggtac gaccttcccg gtcggcccga catcctcagc ctcctgcgcg
    33121 cccttgacgg ccttgcgggg gttcgctgga ttcgcctcat gtacctctac ccggcggccc
    33181 tgacgacaga gttgatcgag tatcttgccg acgacggcac caaggcgctc gactactttg
    33241 atctgccgct ccagcacatc agtgacgata ttcttcgcgc catgcgccgc cgcggcgacc
    33301 gccgcgcgat cgagagccaa ttgcgcgcca ttcgcgagat tgcgccgggc ggcaccgtcc
    33361 gcaccactct catcgttggt ttccccggcg agacggaaag gcacttcgcc gagctgcgcg
    33421 agtttgtgga gcgcttcgag ttcgaccgcc tcggcgtttt cgcctactct ccggaggagg
    33481 gcacgcccgc cgcggagtat cccggacagg tgcccgagcg gatccggcag cggcgcgtcg
    33541 acgacctcat gacggtccag caggagatcg ccttcgccaa gaataattcc ttgatagggc
    33601 ggatcctcga agttatcatt gactcggcgc cggccgatgg tccggcggtc gggcgaaccc
    33661 ggggcgactg ccccgagatc gaccaggaaa tcagcgtctg cggggagcgc ctcgcggccg
    33721 gtaccttctg ccgcgtccgg gtcgaggcgg ccgacgggta cgatctccgc ggatgcacag
    33781 tcggggtcgt ggcatgattc agtttgagaa gctggggata ttcctctcca aaccggtcgc
    33841 cttcgtgctc gtggtgatct acctcctcca gtcgggtctc ctggtctatc tcgtgaagaa
    33901 caagtacgac ctggaacggc agatctcgtt ccagcagacc cgcatcggca cgcttgagga
    33961 gaaactccag atattcaagg ctatcgacga cttccagatc ggcttctccg acgaggagat
    34021 caaccggctc accgacgtca tctacgagga gagcaagaag tacgagtacg acccgctctt
    34081 catgctcgcc atcattctca ccgagtcgtc gttcaagaaa gggcagacct cgccggccgg
    34141 cgcccagggg ctcatgcaag tcatgccgtt cgtcgggcgg gacgtcgccg gccgcagcgg
    34201 cgtcgcctgg cagggggact cgacgctctt ccagcccgac atcaacatcc gcctcggcgc
    34261 cctccacctg tttgagcaga tcctgaaatt cggcgacgtc aagcaggcgc tcgtcgccta
    34321 caatttgggc gagacccgcc tccgggggat cctccgcgac aagcagccgg tcccgaagac
    34381 gtacctgaac aaggtgctgg agaactacaa aatgttgaag gaggcctacc gggtgtgaac
    34441 cggatcaccg ccgccggcgc actcgccggt ctcttgctgg tcgccgcgct gctggcctcg
    34501 tgcggcgcgg gctcccgctc cctgtcccag ctcgacgccc gccagctttt cgacgaagga
    34561 cagcggctct atgaggatgg aaagtaccgc caggcgatcg agtacttcca gaccgtggtc
    34621 tttaactacc cggggcacgc ggtggttgac accgcccagt actacctcgg cctcgcgtac
    34681 tatggcaacg aggacttcga actggcggcg ctggagttcg accgcctcgc gcgcaattac
    34741 ccggcctcgg cctttttcgc caacgccatc ttcatgcgcg gcgtcgccta tttcgactcc
    34801 accccgaaaa accccggtct cgatcagacg gagcttgaga aagccatcgc cgtcttcgag
    34861 gacttcctga ttgatttccc cgaatccccg ctcgttcccg atgtgcagaa gtacctcgcg
    34921 gcggcgcgca cgcggttcgc gaagaagtat ttcaacgccg gcatcgtcta tgagcgcctc
    34981 ggcgctttca aggcggccaa agtgtatttc cagaaagtca tcgacgagta taccgacacc
    35041 gacctggccg cccgggcggc ctttcaccgc gccgatatgg atctccgcct caaggacttt
    35101 gccgccgccc gcaccggttt tgaaagcttc ctggctgcct tccccgaaag cgacctggcg
    35161 ggcaaagcgc gcaagagggc cgtggaatcc gcgtaccgcc agtgccgcct gagctacgac
    35221 aagggcgaca ccgccgaggc gcgccagtgc ctccggcagt tccgggagag ctacccggcc
    35281 aacgggtacg aggatgacgt caatgagatg ctcgaggcgc tcgacaaaca ccccgccggg
    35341 tccgacacca ccagacatgc ggagtcctga gcaggggggc gtgtggggcg tcctcggcgg
    35401 cgccttcgat ccggtgcaca gcgggcacct cgccctggcc cgcgagatcc tccgcgcccg
    35461 caaccttgcc ggcgtcctct tcgtccccgc ctaccggcac ccgttcaagg gtgaccgggc
    35521 ccgcgcctcc tggccccacc gggtcgccat gctccgcctc gctctggccg gggacgaccg
    35581 cttgcgcgtc tgcgagatcg aagccgagca ggagctttcc ggctacaccc tcgacaccct
    35641 gcgggcgctg aagaaggcgt acccccaggc ggccttccgc ttcctcgtcg gcgccgacct
    35701 cctggaccag attgaccaat ggcattcgcc tgaagagctg cttcgcgaga cgccggtttt
    35761 ggccggcgcc cggcccggct accaattgaa ggtcagcccg gggctgcccg ccgaccgcat
    35821 cgagcttgtg ccggccgaaa cgcccgcggt ttcgtcgacc gacctgcgcg ccctgatcgg
    35881 ccgcggcgcg ccgcgccccc ggctgctggc ctggcttccc gcgcccgtgc tcgactacat
    35941 agaaaaagag gggttgtacc gatgacgccc gaccgcaaga ctgtctacct ggtcgatggc
    36001 tcggccatct tctaccgcgc ctacttcgct ttcctccgca acccgctgat caactcgaaa
    36061 ggggagaaca cctcggccgt cttcggcttt gtcaattcgc tgctgaaggt cattcgcgat
    36121 gagaagcccg actacctcgc cgttgcctgg gactccaagg ccccgactgt ccgccacgag
    36181 cgctacgagg agtacaaatc cactcgcgcc aagatgcccg aggacctcgt ggcccagctc
    36241 ccggcgatcc gcgaggcggt cgaggcgttt cgcatccggc actttgagat ggagggtttc
    36301 gaggccgacg acatcatcgg gaccctcgcc agccgctgcg cgaaggcggg cttcgacgtc
    36361 tggtgcgtca ccggcgacaa ggatttcttc cagctcgtta ccgaccgcat ccgcatctac
    36421 acgccccagc cgggcgccga ccaggccgtc aagatgggcc gcgaggaagt aaaggcgaaa
    36481 ttcggcgtct atccggaagc ggtcatcgac aagctcgccc tcatgggcga cagttccgac
    36541 aatgttcccg gcatccccgg cgtcggtccc aagaccgccg atgcgctcct ggagcagttc
    36601 ggcagcctcg aggcggtcct cgccggcgcc aaccggatca aggccaaagc cgtccgggcc
    36661 cgggtcgagg cccacgccga cctcgcccgt ctctccaagg agctggtgac catctgcact
    36721 gatgtcccga tcgcctgtga cctggagcag ctccgcttcc gcgaacccga tcacgccaaa
    36781 ctcgtcgaat tgttccagcg tctcgagttc aaccgtcttc tgaaacaact tgtccccgag
    36841 gcggcggccg ccgcgggcgt caccgacgag gcgcccggcg ccaccaagcc gggctaccat
    36901 gtcgtcgccg ccctcaagga tctcaagact cttctgaaga agctctcgac ctgccggaaa
    36961 gtcgccgttg acaccgagac cacctcgctg aacccgctcg ccgccgagct cgtgggcatc
    37021 tccctgtcgg cccgcgccaa cgaggcctac tacctgccgc ttggccacac gggggagggg
    37081 gcggtgaacc tgccgcttcc ggaggccctc gccgaggtga agaaactcct cgaaaacccg
    37141 gacgtgcaga agttcggcca gaacatcaag tacgatctgg cagtcctgcg caagtacggc
    37201 atcgaaatcg agccggtccg cttcgacacc atgctggctt cgtacgtcat cgacccatcg
    37261 tcgcggcagc actcgctcga tgccctcgcg ctccagcatt tcaaccaccg caagacgccc
    37321 atcaccgacc tcatcggtgc ggggaagaag cagatatcgt tcgcccaggt tccggtcgac
    37381 aaagcgtgcg actattcgtg cgaggacgcc gactacacct accgcctccg cggcaaactc
    37441 gcgccggtga tcgaccagct cggcatgaac agcctctact acgacatcga ggtgccgctc
    37501 atcaaggtgc tcgccgacat ggagcgggag ggagtgcgca tcgacgtctc atttctcggc
    37561 gagttgtcgg tgcagatgga gcgccggctg gaggaaatcc gccgcgagat ctaccgcatc
    37621 gccggtaccg aattcaacat caactccacc cagcagttgg cgcacatcct ctttgagaaa
    37681 ctcggcctcc cgacgaaggg gaagaccacc aagaagacgg gcttttcgac cgacgtccgg
    37741 gtgctcgagg aactggccgc cctccatgaa ctccccgccc gcatccttga tttccgccaa
    37801 ctgaccaaac tcaagagcac ctacgttgac gctctcccga agctggtcga cgggcgtacg
    37861 gggcgggtgc acacgtcctt caaccagacg atcgccgcga ccggccgcct ctcctctacc
    37921 gacccgaatc tccagaatat ccccgtccgc accgacgaag ggcgcgagat ccgcaaagcc
    37981 tttgtcccgc gcgacgaggg ccacaccctg ctcgtggccg attactccca gattgaactg
    38041 cgcattctcg cccacatgtc ggaggactcc accctcattg aggcctttcg acgcggcgaa
    38101 gacatccacg cccgcacggc cgcggaggtc tacggtgtgg agatcggcca agtgaccccc
    38161 gagcagcggc gggccgccaa gacggccaac ttcgccgtca tctacggcgt caccgcctac
    38221 ggcctcgcgg cccagtccgc cctcgatgtc gggcaggcca aggccttcat cgccacctac
    38281 ttcgagcgct accccggcat ccggcgctac ctcgaggcca ccaaggaatt cgcccgtgcg
    38341 cgcggctacg tgacgaccat gttcaaccgg cggcgcacca tccgcgaaat caacgaccgc
    38401 aacgttaacg tccgccagtt tgccgagcgc gcggcgatca acacgcccat tcagggtacc
    38461 gccgccgaca tgatcaagaa agccatgatc cggatccacg ggcggctccg cgccacacgc
    38521 tcccgcatga tcctccaggt gcacgacgag ctcgtcctgg atgtgccccg ggaggagctc
    38581 gacgaggtgc gggagattgt ccgcgacggc atggagcagg cggtgtcgct caaggtcccg
    38641 atcgtggtgt cgctcgggtc cggtcccaac tggctggacg ccaaatgacg ccgccggggg
    38701 cttgcccttt tcccgaataa gcctttatta ccggtatgtt gatcggttta accggattga
    38761 tcggcgcggg gaagtcgacg gccgcccgca ttctcgaatc gttcggcgcc gtggtactcg
    38821 atgccgatga gatcgggcgc gaggtggtgg aggcctccct cccactgcgg caccggctgg
    38881 ccgaagcctt cggggccgac gtgctcaccg tcgacctcga cctcgaccgc aagaaagtcg
    38941 ccgaacgcgc gtttgccggc gaggagtccc gccgccgcct gaatgagatc gtccacccct
    39001 acctcctcca cgagcttgac agccgcatgc gccggctggc caagcgctgc ccggtggtgg
    39061 tggtcgacgc cgcgctgctg accgagtggc gcctgcatca acgcatggac tacacgatcg
    39121 tgatcgatgc ccccgaacag gtccggctcg accggctggc cgcgcggggg atggcgccgt
    39181 ccgaggcccg ggcgatcgag cgcctgcagc cgtccccgca ggacctgcgc gtctgcgccg
    39241 acttcctggt cgtcaacgac ggatccaccg atcgcctcgc cgcaaccttg agcgacatct
    39301 gggagatgat cacccgctcg aaaaggggag acttgcatga agaacccgac accgcaccct
    39361 gagaatgcgc gctgcgacta ccgcgtcgac gaattggtcg agcgcgcgcg ctacatgcgc
    39421 ggcctgaacg aaatcgctct ctgctcggcc cggtcggggc actccggcgg cacgctgagc
    39481 gtgatggata tcgcggccgc cctctatttg agaatcgccc gccacaatcc aaccgacccc
    39541 gactggcccg accgcgaccg catcatctgg tcggccggcc acaaagcccc cgctctctac
    39601 accgccctgg ccgtcgccgg ctatttccca gaacccgagc tcatgaagct ccgcatgctc
    39661 gactccccgc tccaggggca cccccaccgc ctcgacctgc cgggggtgga gatcagctcc
    39721 gggtcgctcg ggcaggggtt ttcagtcgcc gtcggcgtcg ccctcgccgc ccggctcgat
    39781 gggaaggact accgggtgtt tgttatttcg tccgatggcg agcaccagga aggctcgatg
    39841 tgggaggccg ccatggccgc cgcccactat cgtctcgaca acctgatcct catcgtcgac
    39901 aagaaccgcc tgcagatcga cggtccggtt gccgaggtca tgaacatcga cccgctggcc
    39961 gacaaatacg ccgctttcgg ctggcacgtg cgcgaggctg atggccacgc catggccgag
    40021 atcgttgagg ccctcgactg ggcccgcaac cgcaacgact ccggcaagcc ggtcgcgctg
    40081 atcttccaca cggtgaaggg gaagggcgtg tcgttcatgg agaacgtcgt cggttggcac
    40141 ggcaagccgc cgaagctcga cgaactggag cgcctgctgg ccgagctcgg cctcggagac
    40201 accttcgccg tgtccgatct catcgactac ggcgtccgcc accagatcgg cgtcgacgcc
    40261 cgcctgaacg acgaactccc cgcgttctcg cgcgacttct ggtggaatgc cgggccggcc
    40321 atgcgcgtgg acatggatcc tacgcgcaaa gggttcggcc gcgctcttga ggcctgcggg
    40381 agcgacgagc gcatcgtctg cctcggcgcc gatatctccg actccatttg catctccgat
    40441 ttccacaaga accacccgga gcgccgccgc cggacatgga tttatcaaac tgaataaact
    40501 cctc
//
LOCUS       DAOPOE010000016        38379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1428_length_38379_cov_5.240163, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000016 DAOPOE010000000
VERSION     DAOPOE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38379
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_38379_cov_5.240163"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="PKM94_03855"
     CDS             <1..1612
                     /locus_tag="PKM94_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOD65805.1"
                     /translation="TALGFTLANTDGDPANLTENIHNLDIVQASDVAKKTSGSVQIAD
                     AFGNALTIAAAADAASIATGAFAAATAGNVGTYTFTVQYSDVNEGTTSAQTIAVDIEL
                     GDDADAVAAKINTEIGMNSVLSGRISASAAAGVVTLAASNEGAQYSIRTTASSTDATD
                     ALFAVAATSDRGVSANQLTLTATTEANSTVSQTITLTAGTYTSLADIVTEIDTQLGTA
                     FGLVAGGAAGEHDVDAVVTDSDKIQIRTKDEGSAYGLQMGTLGTATGNLEHILGLTED
                     SIANAGTDALIRFDNYTQSVDRVKYGSTWDVEIANKAAGEVGRGTLNLTIAKASTGLN
                     VGSALLDVKAAKFDVRLDGGPATSVTAGVNTTVYNADRSESVVLWYDLTSAGGTETIN
                     NVDRSLVFQIGANVGQTAKIGLRNMAASALGRGFAANMFSSLAEIDVTTVQGAQDAQA
                     IIDAAINEVSTTRGTLGSFQKNTLESNLRNLRIAAENLTASESMIRDTDMAKEMSEFT
                     KNQILVQAGTAMLAQANQVPQIILSLFQ"
     gene            1768..4104
                     /gene="fliD"
                     /locus_tag="PKM94_03860"
     CDS             1768..4104
                     /gene="fliD"
                     /locus_tag="PKM94_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOD65806.1"
                     /translation="MAGIGSIDGIISGLNTTEIVDTIIKGERRNADLLEEEQAKKTAI
                     ISAFKALQAKFLALNTELAQLVRASSYEAAAVTVSDPDVLSATASGRVTGGSYDVQVL
                     SLARNHQLAAQGFAGDTQALLGTGTIRIQTGAGPLKTITIDGTNNSLAGIRQAINDAG
                     AGVTASIVNDGSSSNPYRLVLTADKTGRANRITVTSDLTGGANLNFATPSFDAPEVLS
                     FAGGSTAKVFLGTTAAYTGTANRIYTFTVAGTSTVTVGNEPITLNWTDGTNSGAIVVT
                     QADLETELAGVGSDGLKITLSAGILTGGDTFQVSTFAPTLQEASDARIAIGSQDGQGS
                     PIVVTSATNTFTNVIGGLTLRASQLTAPGETVTVGTERDITAIRERVQNFIKRYNEVI
                     DFIEEQNSYDQEADEAGILFGDATLWMVRNSLSNAVADRIPGIDSKFTQLYALGIRTQ
                     GDGRLAITDAGRFEEALRTNLDDVIALMSRTGSASRTGIEFVSSTAATKVGQGYAVDI
                     TQAATKGYYRGGDLGNLEAAPLVLTGSNNTLKITVDGIKSQELYLTPRSYSSGEALAA
                     EIQARIATDENLGPRGVTVTWVSSAGGGYLTIASASYGSASKVTIEAVDNSAAVALGL
                     AAGESVAGQDVAGTINGETAEGKGQLLTGKTGNATTDGLVLRVTLDETQVISGAEGQV
                     TVTKGVAARLKDVVESLTKAEEGTFDRRIKSYQSQVDTIAKRVAEIDERLKLRRESLL
                     QKYYLMEQTLGEMQSIGNYLESQLAGINTNWGFRTSKQ"
     gene            4128..4562
                     /gene="fliS"
                     /locus_tag="PKM94_03865"
     CDS             4128..4562
                     /gene="fliS"
                     /locus_tag="PKM94_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOD65807.1"
                     /translation="MASSKVAAYRREEMAGKSPLELVIQVYDGAISAFRAAADRYRSD
                     DAAGGREQLERAQTFVTHLYTTLDAERGGDISAQLGRLYAYVINRISVAAATGDPGVI
                     DDNIAILNNLREGWIGLRAQSADPAPSGPAPAAAPGGFVGSA"
     gene            4572..5024
                     /locus_tag="PKM94_03870"
     CDS             4572..5024
                     /locus_tag="PKM94_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65808.1"
                     /translation="MDYDVLKQLERELLAVLKEEYTFYQSLYLTLDKQRDAVRFNRDD
                     HLLDLLAEVERYRQRIARSELTIRQLQEKHPKAFRLAAALPEVKRTINSIRTLVKKSV
                     DVVSECDEYLQGRVNRIKEELGELRNSQQILQYLSEPDPSPQFVDGKK"
     gene            5168..5566
                     /locus_tag="PKM94_03875"
     CDS             5168..5566
                     /locus_tag="PKM94_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65809.1"
                     /translation="MNIGRVRTVRPSLDTARTPGDETTVRRKGRNNAAPEEGGGARKA
                     GTPGDQLVISEDARARLAALADAARAEEILTPPPEDAEGLRMEKMRLASDRMRSGYYD
                     RPEIKRQIADRLADEMCRRSPEQPETPKDE"
     gene            5563..6483
                     /locus_tag="PKM94_03880"
     CDS             5563..6483
                     /locus_tag="PKM94_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD65810.1"
                     /translation="MTHEAVSTHVETPAATIDTRIRQIVSNIRNLPTPPVVFHQIQKV
                     ISDPNASAAQVAAILGEDPAMSVKVLKLTNSVFYGLAREVESVKQAVVVVGMEAIKNL
                     VLSASVLDMFKGNTLDQERQERFWRHSLATAFCSRLIARSVRDRGMIDPDSAFSAGLL
                     HDVGKLVIECFLAQEYAKFAAVRCDNVTATDLEIERETLGYTHDHLGGFLAMQWKLPQ
                     RLGDAIAFHHSPTAAPAPELLACIVHSGNFVAKHTFYGREDQHLIGHPDPEALAKLGI
                     DEGRLEGFADKLRDEYAKAETFMQMLGLTG"
     gene            6824..6900
                     /locus_tag="PKM94_03885"
     tRNA            6824..6900
                     /locus_tag="PKM94_03885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6858..6860,aa:Val,seq:gac)
     gene            6993..8930
                     /gene="thrS"
                     /locus_tag="PKM94_03890"
     CDS             6993..8930
                     /gene="thrS"
                     /locus_tag="PKM94_03890"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOD65811.1"
                     /translation="MQITLPDGSVRPCEQGTTGADLALSISPRLAREAIAVRVNGAVR
                     DLNAPLPSEATVEILTFDHPDGRQVFWHSSSHIMAQAVVELFPNVKLAIGPPIDEGWY
                     YDFDVAKPFAPEDIARIERRMQEIIDEDAEFRCEHRSRTAAIDYYRQRGEPYKVELLE
                     GFADDEVSFYTHSRFIDLCRGPHIPHTGIIKAFKLTATSGAYWRGDEHRPMLQRIYGV
                     SYPKKAMLDAYLQRIEEAKRRDHRLLGKQLELFQISDEIGGGLVLWHPKGAMVRHEIE
                     KFWRDEHLKAGYDLVYTPHIANRELWRTSGHLDFYQESMLGPMTIEEREFQLKPMNCP
                     FHIVMYRSRRWSYRDLPLRWAELGTVYRFEASGVMHGLMRVRGFTQDDAHHFVTQEAM
                     EEEVGWVLDFCVHMLRSFGFEDYDIYLATRPKEKSTGREEDWVRAEASLRQALEVRRL
                     PFDVDEGGGAFYGPKIDIKIKDALNRSWQCSTIQFDFNLPERFDMHYIDADGRPKRPF
                     MIHRALLGSIERFFGVLIEHYAGKFPLWLAPVQVKVLPITDSQNDYGRALADELKRHG
                     IRAQVDDRSDKIGAKIRDAELMKVPYMFVVGGREAENRNVAVRKHGVGDLGVRSLEEA
                     RDMLLAEIRTRGLAQSGAGAR"
     gene            8955..9620
                     /gene="infC"
                     /locus_tag="PKM94_03895"
     CDS             8955..9620
                     /gene="infC"
                     /locus_tag="PKM94_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD65812.1"
                     /translation="MRINRRIRVSPVRLIGPEGEQIGILPTKEALERAETLGLDLVEV
                     SPNSRPPVCRIMDFGKYKYELSKKDRQAKKRQHSFQLKEMRFRPKIDEHDFEFKTKHV
                     RNFLESGSKVRVYVMFRGREMAYQEYGRKVLDRVAEELKDIATVESAPKMEGRNMNML
                     VAPRPEVMKVLKQKAEQRERAELKERERAAEAGEAMPDEQPGELQESPADGGGDRTGE
                     NNE"
     gene            9642..9836
                     /gene="rpmI"
                     /locus_tag="PKM94_03900"
     CDS             9642..9836
                     /gene="rpmI"
                     /locus_tag="PKM94_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOD65813.1"
                     /translation="MPKMRTQRAAAKRFKKTGTGKYKRKRAFKSHILTKKTPKRIRKL
                     RRPAILSHADQGRVKRMLPN"
     gene            9874..10224
                     /gene="rplT"
                     /locus_tag="PKM94_03905"
     CDS             9874..10224
                     /gene="rplT"
                     /locus_tag="PKM94_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOD65814.1"
                     /translation="MARAKNNVAAHRRHKRVLDRASGNWNGRGKLYRTALETVHKGLK
                     YAYRDRRAKKREFRQLWIARISAAARMCDTNYSQLIAGLKTAGVNLDRKMLADIAARD
                     MATFSSLAKMASGK"
     gene            10242..11255
                     /gene="pheS"
                     /locus_tag="PKM94_03910"
     CDS             10242..11255
                     /gene="pheS"
                     /locus_tag="PKM94_03910"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOD65815.1"
                     /translation="MTLPDDIARLEQEAKKRIGSADSLDLLNELRIEYLGRKGGITAI
                     LKGLGQLPPDERKAVGAAANRARAALEQWLAEAAGRLEGAGPRGTIDPTLPGTGQYVG
                     AVHVINQVMGDICRTFYGMGFETAQGPDVETDYYNFEALNFPPDHPARDMQDTLFVEG
                     GRLLRTHTTPVQARVLETRRPPIKCITPGRTFRNEAISTRSHVAFHQVDGFLVDEGVT
                     LADLKGALVAFCRAFYGPDIKLKFRPSFFPFTEPSAEVDISCILCGGTGCQLCKFSGW
                     LEILGCGMIDPNVLTKAGIDSEKYTGYAFGMGVERQALLKYRINDIRLFFNNDIRFLR
                     QFR"
     gene            11259..13643
                     /gene="pheT"
                     /locus_tag="PKM94_03915"
     CDS             11259..13643
                     /gene="pheT"
                     /locus_tag="PKM94_03915"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOD65816.1"
                     /translation="MEVSYQWLIELTGVTWSVEEMARRLTLCGTACEGITPFARHMDR
                     VVVGEVCAVAPIAGADKLRRATVAIGAETLDLVCGAPNVAAGQKVPVALLGAKLAGEV
                     EVRRATIRGVESLGMICSERELGLSDDHSGIMVLEPEMPVGAPLAEALDAADYRMTFE
                     LTPNRPDSMSAIGIARDIAALARVRLRRPAVRLRETAEKAADVISVRLDDPAACPRYA
                     ARVIRNVTLGRSPWWIRRRLMMSGVRPINNVVDVTNLVMLECGHPLHAFDLDRFGSRE
                     VVVRRAREGERFTTLDGKTHVCDPQVLLITNGAVGVAAAGVMGGENSEVAETTRNILL
                     EAAYFDPPTIRKSRRALATQSESSQRFEKGVDPNGIEYAIDRAAALLADLGGGEVLAG
                     MVDAYPRRIDPKAIALRPRRCNDVLGTDLTAAQMKEILEGLEFAVEDGDPLRVTVPTF
                     RPDVEREIDLIEEVVRILGFDRVEDARSTIGPLFTPLHFEDTFKDQVRQVLTGAGFDE
                     VLSHGLADSRLADLAAPELPQLRLVSTASAELNIMRNSMILSAVPILGHNVARRNVDL
                     RIFEIGKVYFPPTPAGEWIEDERILLAVTGDTPHTWREKPRPHDFYDVKAGLERLAEH
                     FHWPALEFVPQAVSFLEPGASFRLRVRETEIGAIGQLSVKLARQADIKQAVWLAEVAT
                     GPLLPLSSRVAAYEPLPIYPAAPRDLALVVDETVPAGDIVGAIRTAAGELAEDVSIFD
                     LYTGKQIEAGKKSIAVAISYRSKERSLSSEEVEAAQQKVVAAVKKLFNAEVRDK"
     gene            13640..13891
                     /locus_tag="PKM94_03920"
     CDS             13640..13891
                     /locus_tag="PKM94_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65817.1"
                     /translation="MNLEEKFQKLLEQVGGLTRQSQTLRKENSDLSRENDSLKRELAG
                     LRAECHRLKLQAADRADLVTSKLSSVLQRLDELERTEGQ"
     gene            14000..14302
                     /locus_tag="PKM94_03925"
     CDS             14000..14302
                     /locus_tag="PKM94_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOD65818.1"
                     /translation="MAANIVVVNIYGEDYPITGVTDAAHISRVADFVNARMQQIAQSS
                     RVKARDKVAILAAMQIASELLEKSEQLESTLGQVEGNLDSLLDRLDRELAPVSDSR"
     gene            14403..15968
                     /gene="rny"
                     /locus_tag="PKM94_03930"
     CDS             14403..15968
                     /gene="rny"
                     /locus_tag="PKM94_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOD65819.1"
                     /translation="MDKIIIVAAAAVVAGVIGYFLSWRAAQRMNREKLAAATVEAERV
                     VEEAKKEAEIRRKEARLEAREEFLKLKGDFEREVAARRQELDAADKRLSELDTNLGRK
                     TEHLDAKEKDLVNREKATQAREKGIALREEELDQIIARQNERLQKIAQMTPEEAKKQL
                     MDNMINAARMEAAQYIKEIREKAEADAEKEAREIILSSIYRCAADHTVETTVSVVNLP
                     NDEMKGRIIGREGRNIRSFETATGIDVIVDDTPEAVILSGYDPVRREIARMALEKLIA
                     DGRIHPTRIEEVVEKAQKEMEVIVREAGEQACFELGIHGLHPDIIKMLGKLHFRTSYG
                     QNVLAHAKEVAMLCGLMAAELELDAPLAKRCGLLHDIGKAIDRETEGTHTQIGADFMR
                     RYKEAPEVINAIESHHGDVPMVGPYPVLVQAADAISGARPGARREPLEAYIKRLQQLE
                     ELADSFKGVAKAYAIQAGREIRVIVENDTLDDLATSILAGDIAARIEREMQYPGQIKV
                     TVVRETRATEFAR"
     gene            16123..17010
                     /locus_tag="PKM94_03935"
     CDS             16123..17010
                     /locus_tag="PKM94_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOD65820.1"
                     /translation="MSLVTVLFIADVCGKVGRQALAHMVKPLRRKYGAHYVIANSENA
                     AGGFGITAEMARKVFAYGVNVQTSGNHIWDRYDIIRYLNENPRMLRPANFPEGVPGRG
                     CSVDRVDGVTIAVINLMGRTYMKDIDCPFQVARRLVEEVRRETSVIIVDMHAEATSEK
                     QAMLYWLDGKVSAVIGTHTHVATADEQVTPKGTAYITDVGMTGPYDSIIGMDKEPSLE
                     RFLTGMPKRFNTAEGDCRLAGAVLTIESETGRAHRIERIMYPFDIATFRPTDIAPADG
                     TEDAAPEEEFDNEADGEEV"
     gene            17010..17915
                     /locus_tag="PKM94_03940"
     CDS             17010..17915
                     /locus_tag="PKM94_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOD65821.1"
                     /translation="MSAKIIDGKEVAASVLDRLQPRIAALRERGVTPGLAAVLVGEDP
                     ASATYVRSKAKKCEEIGLYSEVIRRPADIGQDELMRIVADLNANPKIHGVLVQSPLPR
                     HIDEMAVTLAIRPGKDVDGFHPYNVGMMALGFPRLLPCTPHGIVKLLEFYGLDPAGKE
                     VVVVGRSNIVGKPVAAMLLQKAAWANATVTVAHSRTANLAAVVRRADIVIAAIGRPQT
                     ITGDMVKPGAVVIDVGVNRVEDESAKKGYRLVGDCDYEACAERASWITPVPGGVGPMT
                     IAMLMSNTVAAAELALVGATDYAST"
     gene            18188..21844
                     /locus_tag="PKM94_03945"
     CDS             18188..21844
                     /locus_tag="PKM94_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65822.1"
                     /translation="MKLTLKILLSVLLAARAFGAVDLQAISNTYGPLLREGRLAPPSS
                     AVYVEFREFAVALDDSVAADTARYTLTCRVTAPWILVNSLGGFVFSFPEGFSLHHAEV
                     GALTDDNAGLKFVIQETVIADNVVTIGLGVYGQPGETDSLVTLTLVIADVINPQTAGY
                     YQAAGLAFDRQGMILAGPVLSDPFRIYPRAIQSLEVFPAADTTVTAGTLLAFGAAGYD
                     EFGNRVDDPPVSWSLMEGAIGTVSGNTLATRTVGSGRVQATLGGLTALSGLITVIAGP
                     AALLDFDVPPQQLVGQPLFGPARVLVFDACGNLCGGHDLAADPLEVMGGEGALEPAFL
                     DDNAFISGGIIDLDAAGVVYRGPTAQTTISGRTAYAVSDTEPVSFSGYDFLALLGPDG
                     GTPAALPAESITALRVVVSNRGLLAPAGPAALEADFASSPAGGGTATFVGGSDGRTDT
                     VLLSISTAGLPEGETELTLRLAAEFEIGGRIYPVEQTALYPVTITAPIALDLVSESLG
                     PDTLYPEMPLELGFDVRVSGLALPVDSTRVTIALAGISPALFSGIAAATAGEDGILQY
                     RGLPAAAPAIPAAATPYELTVTLYTDGQIYEAAFTANTAVVLEPVRLTYAPGSLRPRL
                     VPGGYHSAFMFDLDLAVAYDVEWQGVQSAFGLTGVDFAAFASFAPVDGAPAPGLNPMA
                     TEALFVPESQVGGTLACAGTAVVYIPSAGVSDTVALDFGGEGVEVRSLGVMRVAALDV
                     LAPNAPNVNTDQAFRLLARVANISTIDVGPVTLRLTSDGYSTYDSAETVAHIPPGDTA
                     DVYFDCRAAAAPNPGEIFQVRAEPDTPDQAVVQPPDDNVALVRIQLPADLRLTYSLWG
                     VLPAVVVDHSTPFGLTVEIENRGEAAVDSGFYRLLTGGIDFGAPDTLEGPIAVGRHIE
                     FPFTAPSFDTAALFQFRLLRIPHDLNLGAPAHIEEDQFSFILRVESQDADLFVKVTEL
                     GSNLISPGEDRDLFRIDLTNEGSSTVSVIRLERMRMDVRDRTGRPTDAARLFVADRTV
                     VLEDGVPVPAEVTVADQIAVAFDGLTIASGHTRSLTFRGGFVNSLPPPFTLGLETDGF
                     EAVFQGGTHDGFPVRVKGPAGPYLFSRTYVMRGRGLEESYVIENNPFNPKVGPARFEL
                     EIAEPSAVEVRVFTLTGETVYEDEVPQGDVRIEFAWDGRNGEGGMVNNGVYIVAVKVV
                     RTGEEARMKVAVVK"
     gene            21849..23681
                     /locus_tag="PKM94_03950"
     CDS             21849..23681
                     /locus_tag="PKM94_03950"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD65823.1"
                     /translation="MGRRSLSGLAAALLLATAAAVTAGDAGRESPFTVGVGARALGLG
                     GGFTSVADDAATIYYNPAGLTRLDYQEVTAMHMSLLEGTIYDYAAWVYPTVSRGGFGL
                     GVMRLGTGDIVRREEFIETGTFDYVQSQILLSYGRRFHPHFAAGASLKVVYQTLDVYS
                     NWGVGADLGLLARPHEQVSVGMVLRDVIPAGLTLNQEEETAPLSVAGGMSVSGLRIRD
                     DIAGLISFELEQIEDRAVRVHTGGELLFAGAYAVRAGWDRDQLAFGAGFRRGRFQVDY
                     TYKLMDYIPDSHRFSISYDIGPSVAEQAEEQRLAELRKGTDLLEEERRRQFNLYKQKA
                     DEFRARFRLDSALTYYQRALAFDENNQEIIGAIAAMESTQRIQQSEQTDLLERQHELN
                     RMMASYLEQARNFAGKEYYPAALDMIQLIFEVDPNYAPARALKAGIEAAMATAVSETL
                     ETAREARRDGNTVAAIEAYERVLYLDPNNVTAQRGKEALARSLDVGRHLNTGIDLFKA
                     GRYGDARRQFELVLSASPDEPVAQEYLRRIETALEHPPTLDEIQQDPEAWQLYLEGLR
                     FMRNQEYQKAIDAWEKVLERYPGNEATLDNIEQARLRLKSNNQD"
     gene            23791..25833
                     /locus_tag="PKM94_03955"
     CDS             23791..25833
                     /locus_tag="PKM94_03955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD65824.1"
                     /translation="MFRRPVKEINAEVVAEEKYLDSLQRITREACLSAGLSRKEVSPI
                     LLAIEEGATNIIRHAYLYEKGTIRLRIVIYKKMIVFSLIDTGRSYQPESDGKLDLQRL
                     VESGRKGGLGFYMIKKIMDSVEYISSAGYNELRMIKQAQPVASESIPFLRHMATLRAK
                     FSVWTFLIVLVIVGGAYYYFDSQTTRQMKSHLDETVASLVNTIGDQAAGYVINRRSDV
                     EFDELIVSAVRSNAGILRQVVLTDTAGTILAHSDDIHNIRKPYAPPPEVHAARVGVPF
                     RFSENGRALSYLMLPIRQGDRSFGTVHAVYSSEQLQQRLAEARRRILVLTFVLLMVGV
                     AGIWVLSNYFVDPIVRITQRVRRFASGDLRSELSLEGAEEFYEISRAFNDLMTRVSQD
                     RENIVAREKMVKEIEVASQIQKTLMPRRLPDLPGLEIDTFYRAAAVVGGDLYDVFEID
                     PGMYCLAVADVSGKGVPASLVMSMLRTVIQIQAGQSHSSRRTLVLVHEYLRENIPPGM
                     FITIMLAVYDSARRRLNFVSAGHNPLLYYHAASGEIRPLNPAGMPMGVPAAIDTTFEQ
                     RLEQYELELQDGDLFFMFTDGITEASSREGQYYGIDRLIRFMKAQLAQPDVKLPGISA
                     ALMAELEEFCGLSGPADDITFIIGRAALGRLADPPEGTADGARERPSNPTAAGGPN"
     gene            25922..26308
                     /locus_tag="PKM94_03960"
     CDS             25922..26308
                     /locus_tag="PKM94_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD65825.1"
                     /translation="MENISISLSESERDNISEIRVDGVIDTTTAGELEEVMDALMKRR
                     RYRVVVDLAGVDYISSAGWGIFISHIKDVRNLGGDIKLANMVPNVYEIYELLEFDRVL
                     RAHESVDAARREFGAAESGEAKKKAE"
     gene            26323..26616
                     /locus_tag="PKM94_03965"
     CDS             26323..26616
                     /locus_tag="PKM94_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65826.1"
                     /translation="MVDDLGVETADRWGPRGIGPADEVLNGGDLDTAVLRLVKDDPFA
                     SISELSRAVRETGLFPDAGWWRIFGVLRRHGLLSRRSRFRFGRGRGRGPARRF"
     gene            26662..28305
                     /locus_tag="PKM94_03970"
     CDS             26662..28305
                     /locus_tag="PKM94_03970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOD65827.1"
                     /translation="MDDQTVLTVALALLVVVLAALLVHRIRRERRQVEGLRRMKLEEF
                     SEFLRRNSVEGNIVEVARKVSDLLRTAFGCDLILFLRKKRDFLELSYHHGLRDIDRAQ
                     LRAAAERGLLAALATDFRPAPLDRLQGHFADDYLERLRTHGFGLYFPVFWRDNLYGLY
                     FVRSTLETQSPSFHVLVASLAHSLAAAYHIKWHESRYDLVREQLERARASAQKPPAGE
                     GRAASHLLRLVTHRNSQTLLPKLVESIRKDLGLERVAYLYQPNGGGEPQVVQDGVRMN
                     LPAQEKGQVEGVLRAIKNEDVYTLARLAQEQPGLREWVKSLREAGLDHVTAFPAIQGR
                     PALLAYSDRTGAVPREQIDTVRGHVRDLIANVETYEKIEELSQTDALTGLANQRYFKR
                     RLEEEVQRAKRYGRRLALIFFDLDGLKQTNDTYGHLAGDSLIRQMGRILRNNIRSIDI
                     IARYGGDEFCIIMPEADRAMSIRFMERVKIEVAKAEFPVTGLAAPLTSTASLGGALYP
                     DHADNPRQLIFAADMALLKAKEQGRNKAVLYGDAPAESP"
     gene            28473..29429
                     /locus_tag="PKM94_03975"
     CDS             28473..29429
                     /locus_tag="PKM94_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD65828.1"
                     /translation="MNTRLLLLSSLLVLTACGIGSGQEYRIGPGDVLEIEFWQDPTLN
                     STLRVSFDGTITLDIIGEIKASGRTTQELQEDIVRQMSRLNQRISQAVVRVQEYNYLY
                     VFVSGQANHPGKMTFEEIPDLWTIINEAGGVTETGDLSRVTIIRGGEEAGRVEVVNVA
                     AAIAAGQLGGLPKIRRGDTIDIPRTPVGLPSASFDRSAERKNLVYVVGAVTTPGPVEY
                     QPNIDVLEAVALAGGPTNEANVKKARVVCKDEFYGQALEIDLERYSRSGAPARYVLGR
                     EDVVVVPHKSGFFQGFNVTTVATLAGVITTSILVYDQLRRDD"
     gene            29442..30944
                     /locus_tag="PKM94_03980"
     CDS             29442..30944
                     /locus_tag="PKM94_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD65829.1"
                     /translation="MQTPQEKPLNLKDIAAILLKRKWLVIIPIIAVTGLAFASTYLMT
                     PVYRSSTIIWIDQPSNVSRELGMILGREQEGRVSSEEQRRQVQALQNELTSQTYLFQL
                     IKELGLDNDPEVTRQAAKMREANLSYSLDQLKLNLLVDDIRGRVEVSFVGKDQIELAV
                     KSSDPVQAKDMVVSLARIMEQERTKYELEKILDNQDFADLQLQKTEFRYRQMLDSLTA
                     AQARLQSLQLPENISAEANRRDIVTDIDKTQLEIDDYSDERDELRGRLAELALASARL
                     KYTDSIVELRTEIDGQMTRFATMMEKYAWNEQNVINVNVRLNDNLRRLEGAIQQAVDN
                     QFASYEASQRDLLKRYFIAEEHLDVARSRKSQLETSLANIDQRVNRLPRLQAEIQELE
                     RRVDDARKYRDAFKSEETTVGILSERTKERTTYRVIEPARVPVAPEWPNRKKIMVFGV
                     VLGFLLGGAAVFLAEIFDTSVKRIEDIEERLGLPVLATIPRIDKLRSVRR"
     gene            30948..32069
                     /locus_tag="PKM94_03985"
     CDS             30948..32069
                     /locus_tag="PKM94_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65830.1"
                     /translation="METYRLKSRLVDHDREFVIQTANDANLGAISSEIYIDGRLAEAA
                     SQPHPPDIRAEEVLSLLKETHGERKKELETLLGAYRRIMNEGNPEMMLHLGTALFHRN
                     FFADARSVFQAAVALDAKNHLARAYLGMTCLALDEVDEAVAAAEAAATARPGYADYRN
                     GCGEALLAAGQFERAHREFSEAVKINLYYGDAYFNLGLTYVGLASTNEMVKDSRALLN
                     KAADCFRRAAMVNVGYDAGYFDDGMRALAAGEATRAWAAMNEVKSAWKARRRRELAGF
                     HMRYVLHPEWVSEQMIEERIRYLEGEVRRNPNYVDLYTELGRCYLERARLGWQKGIAQ
                     YRRATELNPALTQAVERLRAAEDEYERMRSTLEAMGPKG"
     gene            32077..32874
                     /locus_tag="PKM94_03990"
     CDS             32077..32874
                     /locus_tag="PKM94_03990"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOD65831.1"
                     /translation="MARKPLSIIDLFSLDQPFATEFRRLLHRLRKHGESRETKAIMIT
                     SAMLAEGKSTIAALLALTAARQKGLKTLLIDSDLRRPSIHKFFSTERAPGLADTLGNG
                     VAAADVIRKTALENLHLITAGRATPQPAEVFDAEAIGTIIEEMKFFYDLIIVDAAPLL
                     PVSDPLLLAAKVDGLLLVVKAGSTQREVVERAVDMLELHRDRVLGVILNNVNNSLPYY
                     YDYRYYGYQYPTDKPTEGSAEAGRRHWRRGSRGAAGPSKDNPIKQQH"
     gene            32899..33762
                     /locus_tag="PKM94_03995"
     CDS             32899..33762
                     /locus_tag="PKM94_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOD65832.1"
                     /translation="MNILTVDLEEWFVVEALAERLESRQWDGLESTVAKNCHRLLELF
                     GRHRVSATWFVLGWVAQRHPELIAEIEAEGHEIACHSYYHRRVDTLTPEEFRADTLRA
                     LEAITAAVQRRPRGYRAPSWSINDSIPWAFSILAELEFDYDSSIYPIKHDIYGMPHGP
                     RVIHRMHLEDDRLLYEVPASTYRLLGSNIPIGGGGFLRHSPYWYSKHIIRRLNTSGVP
                     AVVYMHPWEIDPDPPKLAGLTAMQRLRMYGSTSIFYHKLDRLLSDFEFCALADYLRLL
                     KRRQIGFLERP"
     gene            33871..34557
                     /locus_tag="PKM94_04000"
     CDS             33871..34557
                     /locus_tag="PKM94_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65833.1"
                     /translation="MLKRLLLAVLALAVCVIPAEGAKKKPKAGAIKDGWYVDAKYDFK
                     FQVPGNWSVAIKDGDDPVRAVLVQKNYQIPPEYLDAEDYTEVPRLTVYVLQTTMSALE
                     FRDSLLSKTYESDQKKDIRKYFEILNDVSIGEGTRREPVVTKSRELLYLDSLPASLWF
                     GEAQYVKNVTLSAAAQSGKRVNGDYAGTIVAVKNGDNLLLFHLMSEGMYHSSVWQEIR
                     TMIEGLKWPR"
     gene            34682..35047
                     /locus_tag="PKM94_04005"
     CDS             34682..35047
                     /locus_tag="PKM94_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOD65834.1"
                     /translation="MWAVRAVLITVIVIAVVAFAIHNVGEYQKVDVNLIWKSYHQVAL
                     VEVVFWSFVAGIAVSLVMFVSVYIRLSMAGRAMRKQVRALEAELAVLRNRPIEESAQV
                     LDEDVPGRRDLTSPFDERE"
     gene            35050..36144
                     /locus_tag="PKM94_04010"
     CDS             35050..36144
                     /locus_tag="PKM94_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65835.1"
                     /translation="MAEYVLAFLILLAASIIFYLGYDRLVRRTPMPSTALYVEALRDL
                     LDGQRVRAFGKLRQVVNEDYHNIDAYIRLGQILREGNKPDRALQIHKDLTLRAGLPPS
                     QKMGILHQLAEDYSELKDYDMAAAAIKELISLDGNNRWAHIKLLKIQEAKQSWDEAFD
                     TAAHILKLENNKSKKPLAFYKYQQGMQLYKQREYHKARILFKEAIGLNPAYAPAYLAV
                     GDSYRLEERHEDAVNFWVKLIEAVPDKGHLVIDRLKKALYDLGRFSELADICHNILAH
                     DSGNVEARLSLAEFYHAKGEIDQAETLLAEVVERSPENLKAIIGYLRLLVEKGNRRQI
                     DRFFRQLEGRLEKPAADARTAPGGKMIGTA"
     gene            36176..37177
                     /locus_tag="PKM94_04015"
     CDS             36176..37177
                     /locus_tag="PKM94_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD65836.1"
                     /translation="MKKLLCMVMLAAAPAAPASADDLIYRLITQGKLDEARQELSRVS
                     TASVRDGATLFFQALLEPDAVRAAELLEVALGAGVGAEYRELANLRLAQYYSLTKAWD
                     KLERVIVNYRAKFEHGRYREQMMRFSTLLDEQAGAGESALRQADRYLVEYGSGLAQQW
                     GQIDKARVLQGSKKEIGADKMLRGLAREKTGPGVPQALYLLAMDAARRGKADDAVFYY
                     NVLREAYPNAVGLEALVDRLAVMAEGVERRAAEAEQVTGTYYTIQVGVFTDADNARTQ
                     AERFRQTGWKTEIADKTISRVTYRAVYVGRFAAYQEAAAAKARLEREMNETYQVVAR"
     gene            37174..>38379
                     /locus_tag="PKM94_04020"
     CDS             37174..>38379
                     /locus_tag="PKM94_04020"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD65837.1"
                     /translation="MTGPRDTDDSLTPLMRQYYAVKAQHPGKILFFRMGDFYEMFGDD
                     AVKAAPILGIALTSRSHGANERVPLAGVPYHSADKYLARLLAAGEKVVVVEQVEDPRT
                     AKGLVKREIVEILTPGTATIEGEASDRQAVWLAAVCPRGDSALGLAALDLSTGAFLVD
                     EGPTDEVRERLRVLEPREVLYPTGGEAEDGRPGFDPANGAIHLTGFAEWNFDYRTAVR
                     ELSEHFGTSTLDGFGIGEARLGIAAAGAVFRYLRENHRERLSHIDRIVRFDTAEYMVL
                     DYGTVRNLELLRNLAEGTEPNSLFAAVNRCRTAPGARRLAAALVRPFRNKARIEKRLS
                     GVAELVRDRDLAARVREHTQRLPDLEKIAGRLGIGKLNPRQMKAIGEGLAECRALGRI
                     GLVDRLVQRV"
BASE COUNT         7221 a        12114 c        13000 g         6044 t
ORIGIN      
        1 gaccgcgctc gggttcacgc tggcgaacac ggacggcgat ccggccaacc tgaccgagaa
       61 catccacaac ctcgacatcg tgcaggcctc cgacgtggcg aagaagacct ccgggtcggt
      121 gcagatcgcg gacgctttcg gcaacgctct gacgatcgcg gcggccgccg atgcggcctc
      181 gatcgcgacg ggcgcgtttg cggcggcgac cgccggcaac gtcggcacct acaccttcac
      241 cgtccagtat tcggacgtga atgaaggcac gacctcggcc cagacgatcg cggtcgatat
      301 cgagctcggc gacgacgccg acgcggtggc ggccaagatc aacaccgaga tcggcatgaa
      361 ctcggtgctc tccggccgga tcagcgcctc ggcggccgcc ggcgtggtca cgctggccgc
      421 ctccaacgag ggcgcgcagt attcgatccg gacgacggcc tcgtcgaccg acgcgaccga
      481 tgccctgttt gcggtcgccg cgaccagcga ccggggtgtg agcgcgaacc agctgacgct
      541 gacggcgacc acggaagcga actcgaccgt gtcgcagacg atcacgctga ccgcgggcac
      601 ctacacctcg ctggccgata tcgtgaccga gatcgacacc cagctcggga cggccttcgg
      661 cctggtggcc ggcggcgctg cgggcgagca tgatgtcgat gcggtggtca ccgacagcga
      721 caagattcag atccgcacga aggacgaagg ttcggcctac ggcctccaga tggggacgct
      781 gggcacggcg accggcaatc tcgagcacat cctgggcctg accgaggact cgatcgccaa
      841 cgccgggacc gacgccctga tccgcttcga caactacacc cagtcggtgg accgggtgaa
      901 gtacggctcg acgtgggacg ttgagatcgc caacaaggcg gccggcgaag tgggccgcgg
      961 gacgctgaac ctgacgatcg ccaaggcctc gaccggcctc aacgtcggca gcgcgctgct
     1021 cgacgtcaag gctgccaagt tcgacgtgcg cctcgacggc ggcccggcga cctcggtcac
     1081 cgcgggtgtg aacaccacgg tctacaatgc cgaccgctcg gagtcggtag tcctctggta
     1141 cgatctgacc tcggccggcg ggaccgagac gatcaacaac gtcgatcgct cgctcgtgtt
     1201 ccagatcggc gccaacgtcg gccagacggc caagatcggc ctgcgcaaca tggcggccag
     1261 cgcgctcggg cgcggcttcg cggccaacat gttcagctcg ctcgccgaga tcgacgtgac
     1321 gacggtccag ggggcgcagg acgcccaggc gatcatcgac gcggcgatca acgaggtgtc
     1381 gacgacccgc ggcacgctgg gttcgttcca gaagaatacc ctggaatcca acctccgcaa
     1441 cctgcggatc gcggccgaga acctcaccgc gtccgagtcc atgattcgcg acaccgacat
     1501 ggcgaaagaa atgtcggagt tcacgaagaa ccagatcctg gtgcaggccg gcacggccat
     1561 gctggcccag gccaaccagg ttccgcagat cattctgtcg ctgttccagt aagcgaaccg
     1621 cggccgaacc acatgtgccg ggggaggcca atgcctcccc cggccacttg accggaacgg
     1681 cccgaccgcc gcggcggccg ggcgccggac cgaacgggga gacggaagga caatgacagt
     1741 gacgtcgccg acatcaggag gagcgggatg gccgggatag gatccatcga tggaataatc
     1801 tccggtctca acacgaccga gatcgtcgac accatcatca agggggagcg gcgcaacgcc
     1861 gacctgctcg aagaggagca ggcgaagaag acggcgatca tttccgcgtt caaggcgctc
     1921 caggcaaaat tcctcgcgct caacaccgaa ctggcccagc tcgtgcgggc ctcgtcctac
     1981 gaagcggcgg ccgtgaccgt ctccgacccg gacgtgctct cggcgacggc gagcgggcgg
     2041 gtcaccggcg ggtcgtacga cgtccaggtg ctgtcgctcg cgcgcaacca ccagctcgcc
     2101 gcccaggggt tcgccggcga cacccaggcg ctgctcggga ccgggacgat ccggattcag
     2161 accggcgcgg gaccgctgaa gacgattacg atcgacggaa caaacaactc gctcgcgggc
     2221 atccgccagg cgatcaacga cgccggcgcc ggggtcaccg cgagcatcgt caacgacggc
     2281 tcgtcctcga acccctaccg gctcgtgctc acggcggaca agacgggacg ggcgaaccga
     2341 atcaccgtga catccgacct gacgggcggg gcgaacctga atttcgcaac gccgtcgttc
     2401 gacgcgcccg aagtactgtc gttcgcgggc gggtcgacgg ccaaggtgtt cctcggcacg
     2461 accgcggcgt acacgggaac ggccaacagg atctacacct tcaccgtggc cgggacctcc
     2521 acggtcacgg tcggaaacga gccgatcacg ctgaactgga ccgacgggac caacagcggc
     2581 gccatcgtcg tcacccaggc cgacctggag acggaactgg ccggggtcgg atcggacggg
     2641 ctcaaaatta ccctgtcggc cgggatcctg acgggcggcg acacgttcca ggtgagcacg
     2701 ttcgccccca cgctgcagga agcctcggac gcccgcatcg ccatcggctc gcaggacggc
     2761 cagggatccc ccatcgttgt gacctcggcc accaacacct tcaccaacgt gatcggaggg
     2821 ctgaccctgc gggcctcgca gttgacggct cccggggaga cggtgacggt cggcacggag
     2881 cgggacatca cggcgatccg cgagagggtg cagaatttca tcaagcgcta caacgaagtc
     2941 atcgatttca tcgaggaaca gaatagctac gaccaggagg ccgacgaggc ggggatcctg
     3001 ttcggcgacg cgaccctgtg gatggtgcgg aactcgctga gcaacgcggt cgccgaccgc
     3061 attccgggga tcgactcgaa attcacccag ttgtacgcgc tgggaatccg gacgcagggg
     3121 gacggacggc tcgcgattac cgacgcgggt cgtttcgaag aggccctgcg caccaatctc
     3181 gacgacgtca ttgcgctcat gtcgcgaacc gggtccgcct cgcgcaccgg catcgaattc
     3241 gtctcctcga cggccgcgac caaagtcggc caggggtacg ccgtcgacat cacccaggcg
     3301 gcgacgaagg ggtactaccg ggggggggat ctggggaatc tcgaggctgc gccgctcgtg
     3361 cttacggggt cgaacaacac cctcaagatc acggtcgacg ggatcaagtc gcaggagctg
     3421 tacctcacgc cgcggagcta cagctcgggc gaggccctgg cggccgaaat ccaggcgcgg
     3481 atcgccaccg atgagaacct cggaccgcgc ggggtgacgg tcacctgggt gagcagcgcc
     3541 ggcggcgggt acctgacgat cgccagcgcc tcgtacgggt cggcctcgaa agtgacaatc
     3601 gaggcggtcg acaattcggc cgccgtggcg ctcgggctcg ccgccggaga gagcgtcgcc
     3661 gggcaagatg tggccggaac catcaacggc gagacggccg agggcaaggg gcagttgctg
     3721 accggaaaaa cgggaaacgc gaccaccgac ggcctggtgc tccgggtgac gctcgatgag
     3781 acgcaggtga tctccggcgc cgaagggcag gtgacggtga ccaagggcgt ggccgcccgc
     3841 ctcaaagacg tcgtcgagag cctgaccaag gccgaggagg ggactttcga ccggcggatc
     3901 aagagctacc agtcccaggt cgacacgatc gccaaacggg tggcggaaat cgacgagcgg
     3961 ctcaagctcc ggcgcgagtc gctcctgcag aaatactacc tcatggagca gacgctcgga
     4021 gagatgcaga gcatcggaaa ctacctcgaa tcgcaactcg cgggcatcaa cacgaactgg
     4081 ggatttcgaa cgagcaagca gtagtgcctt cgggaggaga acaacagatg gcgagcagca
     4141 aagtggccgc gtaccggcgg gaggagatgg cggggaagtc gcccctggaa ctggtgattc
     4201 aggtgtacga cggggccatc agtgccttcc gggcggcggc cgaccggtac cggagcgacg
     4261 acgcggccgg cggtcgggag caactggagc gggcgcagac gttcgtcacc cacctctaca
     4321 cgaccctgga cgccgagcgg ggcggcgaca tctccgcaca actcgggcgg ctgtacgcct
     4381 acgtgatcaa ccggatcagt gtggccgcgg ctaccggcga cccgggcgtg attgacgaca
     4441 acattgccat actcaataac cttcgcgagg gctggatcgg ccttcgcgcc caatcggccg
     4501 atccggcgcc gagcgggccg gccccggcgg ccgcgcccgg cggtttcgtc ggctcggcat
     4561 gagagagcgc catggactac gacgtgctca aacaactcga acgcgaactg ctggcggtgc
     4621 tcaaagaaga gtatacgttc taccagtcgc tctacctcac cctcgacaag cagcgggacg
     4681 cggtccgctt caaccgcgac gaccacctcc tggatctgct ggccgaggtc gagcgctacc
     4741 gccagagaat cgcccggtcg gagttgacga tccggcagct tcaggaaaaa caccccaaag
     4801 ccttccggct cgccgcggcg ctcccggaag tgaagaggac gatcaacagc atccggacgc
     4861 tggtcaaaaa gagcgtcgac gtggtgtccg aatgcgacga atacctgcag ggacgggtca
     4921 accggatcaa ggaggagctc ggggaactgc gcaacagcca gcagattctc cagtacctca
     4981 gcgaaccgga tccgtcgccg cagtttgtgg acggaaagaa gtagggagtc ggccccgccc
     5041 gccgggcggg ggcagagatg agagggccac gggtgcggtc ggcgcgacgc gcggagcgcg
     5101 ccataggaag gtaacgccgg cgggtgcgcc ggatagaagc aggactttca caccatgagg
     5161 cagccgtatg aacataggaa gggttcggac ggtgcggccg tcgctcgaca cggcgcggac
     5221 gccgggcgat gagacgacgg tgcgcaggaa aggcaggaac aacgccgcgc cggaggaggg
     5281 cggaggagcg cggaaggccg gcacgcccgg cgatcaactg gtgatctcgg aggacgcccg
     5341 ggcacggctg gccgcgctgg ccgacgccgc ccgcgcggag gaaatcctca cgccgccccc
     5401 cgaagacgcc gagggactga ggatggagaa gatgcggctg gcgagcgacc ggatgcgctc
     5461 cgggtactac gaccggcccg agatcaagcg acagatcgcc gaccgcctgg cggacgaaat
     5521 gtgccggcgg tcgccggaac aacccgagac cccgaaagac gaatgacgca cgaagcagtg
     5581 agcacgcacg tggaaacgcc agcggcgacc atagacacgc ggattcgaca gattgtctcg
     5641 aatatccgca atctgcccac cccgccggtg gtcttccacc agattcagaa agtcatcagc
     5701 gaccccaacg cctcggcggc ccaggtggcg gccattctcg gggaagaccc ggcgatgtcg
     5761 gtcaaagtgc tcaagctgac caactcggtg ttctacgggc tggcgcgcga ggtggagtcg
     5821 gtcaaacagg cggtcgtagt ggtcggcatg gaggcgatca agaacctcgt gctctcggcc
     5881 agcgtgctgg acatgttcaa gggaaacact ctcgaccagg aacggcagga acggttctgg
     5941 cgccactcgc tggccaccgc cttctgctcc cgcctcatcg cgcggagcgt gcgggaccgg
     6001 ggtatgatcg atcccgactc ggccttctcc gccgggctgc tccacgacgt gggcaagctg
     6061 gtgatcgagt gcttccttgc gcaggagtac gccaagttcg ccgccgtccg gtgcgacaac
     6121 gtgaccgcga ccgacctgga aatcgagcgc gagacgctcg ggtacaccca cgaccacctc
     6181 ggcggctttc tggccatgca gtggaagctg ccgcagcgcc tgggggatgc gattgcgttt
     6241 caccactcgc ccaccgcggc cccggccccg gagctgctcg cctgtatcgt ccactccggc
     6301 aacttcgtcg ccaagcacac gttctacggg cgcgaggacc agcatctcat cggccacccc
     6361 gatccggaag ccctggcgaa gctggggatc gatgagggcc gattggaggg attcgctgat
     6421 aagctgcggg acgagtacgc gaaggcggag acgttcatgc agatgttggg gctgacgggg
     6481 taggggcgcc ccgctgagag ttcgacgacg accccgcgcc ccacggcgcg gggtctttcg
     6541 ctttcaccaa gggtaatgtc cggtctcctt gcccggaaag gcgggccggt accgcctcgc
     6601 gtgtcccccg tttgtggtcc cgcaattccg aggaaccggg tggggcggcg atggttacag
     6661 gattcgaagc tgaccggtgg cccgacgtcc ttggcggagt cgcgattcgg ccggcgcggg
     6721 tcttgcccgc ggccgggaaa attcggcttg acaactgttc gcccagggta taaaataatc
     6781 aactgcccgc cgatagctta agttggtggg gaacaacgga catgggcgat tagctcagtt
     6841 ggttagagta cttgcttgac atgcaagggg tcactggttc gattccagta tcgcccacca
     6901 ttagtgagtc caaacgatga gcgggtttgc cagtggggga accccacccg tcaatccgct
     6961 cgtgctgtaa acggaaaaga ttcgtggcga ccatgcagat cacattgccc gacgggtcgg
     7021 tgcggccctg cgaacaggga accaccgggg ccgacctcgc cctgtcgatt tccccgcgcc
     7081 tggcccggga ggcgatcgcg gtgcgggtca acggcgcggt gcgggatctc aacgcgccgc
     7141 tgccctccga ggcgaccgtc gagatcctca cgttcgacca ccccgacggg cggcaggtgt
     7201 tctggcactc cagctcgcac atcatggcgc aggccgtggt cgagctgttc cccaacgtca
     7261 agctggcgat cggaccgcct atcgacgagg ggtggtacta cgatttcgac gtcgccaagc
     7321 cgttcgcgcc ggaggacatc gcccggatcg aacggcggat gcaggaaatc atcgacgaag
     7381 acgcggagtt ccgctgcgag caccggtcgc ggaccgcggc catcgattac tacaggcagc
     7441 gcggggagcc ctacaaggtg gagctgctcg aggggttcgc ggacgacgag gtcagtttct
     7501 acacccactc gcgctttatc gacctgtgcc gcgggccgca cattccccac accgggatca
     7561 tcaaggcctt caagctcacc gcgacctcgg gcgcgtactg gcgcggcgac gaacaccgcc
     7621 ccatgctcca gcgcatttac ggggtgtcgt atccgaagaa agcgatgctc gacgcctacc
     7681 tccagcggat tgaggaagcc aagaggcggg accaccgcct gctgggcaaa cagctcgagc
     7741 tcttccagat ttccgatgag atcggcgggg gactcgtcct gtggcatccg aaaggggcca
     7801 tggtgcggca tgagatcgag aagttctggc gcgacgagca tctgaaagcg ggctacgacc
     7861 tcgtctacac cccgcacatc gccaaccgcg agctgtggcg gacgagcggc cacctcgatt
     7921 tctaccagga gtcgatgctc gggccgatga cgatcgagga gcgcgagttt cagctcaagc
     7981 cgatgaactg ccccttccac attgtcatgt acaggtcccg gcgctggtcc taccgggatc
     8041 tcccgctccg gtgggccgaa ctcgggaccg tgtaccggtt cgaggcctcg ggggtgatgc
     8101 acgggctgat gcgggtgcgc gggttcaccc aggacgacgc ccaccacttc gtcacccagg
     8161 aggccatgga ggaggaggtc gggtgggtgc tggatttctg cgtgcacatg ctccgctcgt
     8221 tcggcttcga ggactacgac atctacctgg ccacccggcc gaaggagaaa tcgaccggtc
     8281 gcgaggagga ctgggtgcgc gccgaggcga gcctgcggca ggccctcgag gtgcgccggc
     8341 tgccgttcga cgtggacgag gggggcgggg cgttctacgg ccccaagatc gacatcaaaa
     8401 ttaaagacgc gctcaaccgg tcgtggcagt gctcgaccat ccagttcgat ttcaacttgc
     8461 cggaacggtt cgacatgcac tatattgacg ccgatggccg gccgaagcgg ccctttatga
     8521 tccatcgcgc cctgctcgga tcgatcgagc ggttcttcgg cgtgctcatc gagcactatg
     8581 ccgggaagtt cccgctctgg ttggcgcccg tgcaggtgaa agtgcttccg atcacggaca
     8641 gccagaacga ctacggccgg gcgctcgcgg acgagctcaa gcggcacggc attcgcgcgc
     8701 aggtggacga ccgatcggac aagatcgggg ccaagatccg cgacgccgaa ctgatgaagg
     8761 tgccctacat gttcgtggtc ggcggccgcg aggccgagaa tcggaacgtg gcagtgcgca
     8821 aacacggcgt cggggatctc ggcgtccggt ccctggagga ggcccgcgat atgctgctcg
     8881 cggaaatccg gacgagggga ctggcccaga gcggcgccgg cgccaggtaa ggaggttcta
     8941 tcagcagcaa agacttgcgc atcaatcgcc gcatccgggt gtcgcccgtg cggctgattg
     9001 gtcccgaagg cgagcagatc ggcattctgc cgaccaaaga agcgctcgaa cgggcggaaa
     9061 ccctcgggct ggacctcgtg gaagtgtcgc ccaacagccg ccccccggtc tgccggatca
     9121 tggatttcgg caagtacaag tacgagttgt cgaagaaaga ccggcaggcg aaaaagcggc
     9181 agcactcgtt ccagctcaag gagatgcgtt tccggcccaa gatcgacgaa cacgacttcg
     9241 agttcaagac caaacacgtg cgcaacttcc tcgagtcggg gagcaaggtc cgggtgtatg
     9301 tcatgttccg cggtcgggaa atggcctacc aggagtacgg gcgcaaggtg cttgaccggg
     9361 tcgcggaaga actgaaagac atcgccaccg tcgagagcgc ccccaagatg gaaggccgga
     9421 acatgaacat gctggtggcg ccgcggcccg aggtgatgaa ggtgctcaag cagaaggcgg
     9481 aacagagaga gcgggccgag ctcaaagagc gggagcgcgc ggccgaggcg ggcgaggcca
     9541 tgcccgacga acagcccggg gagctccagg aatccccggc cgacggcggc ggcgaccgga
     9601 ccggtgaaaa taacgagtaa cgagagaagg atagcagcac catgcccaag atgagaaccc
     9661 agcgcgcggc cgcgaagcgg tttaagaaga ccgggaccgg caagtacaag cgcaaacggg
     9721 cgttcaagag ccacatcctg acgaagaaaa cgcccaagcg gatccgcaaa ctgcgccggc
     9781 cggcgatcct ctcacacgcc gaccaggggc gggtcaagcg gatgctgcct aactgagatt
     9841 tgacgacagt cgtaccgaag gagtaccacg cagatggcac gcgcgaaaaa caacgtggcc
     9901 gcccaccggc ggcataaaag ggtgctggac cgggcctcgg gcaactggaa cggccgcggc
     9961 aaactctacc gcaccgccct cgaaacggtc cacaaaggtc tcaagtacgc ctaccgcgac
    10021 cgccgggcaa aaaaacggga attccgccaa ctgtggatcg cccgcatctc ggccgcggcc
    10081 cgcatgtgcg acaccaacta ctcgcagttg atcgcggggt tgaagacggc gggggtgaac
    10141 ctcgaccgga aaatgctggc ggatatcgcc gcccgcgaca tggcgacctt ctcgtcgctg
    10201 gccaagatgg cgtcgggcaa gtagatccgg gaccgcgtga catgacgctc ccggatgaca
    10261 tcgcgagatt ggagcaggaa gcaaagaagc gaatcggctc cgccgattcg cttgatttgc
    10321 ttaatgaact gcgcatcgag tacctcgggc gcaagggcgg gatcaccgcc atcctcaaag
    10381 gactcggaca gcttcccccg gacgagcgca aagcggtcgg ggccgcggcc aaccgggccc
    10441 gcgccgcgct cgagcagtgg ctcgccgagg cggccgggcg cctcgaggga gccggcccca
    10501 gggggacgat cgatccgacg cttcccggaa ccgggcagta tgtcggcgcc gttcacgtga
    10561 tcaaccaggt gatgggggat atctgccgga cgttctacgg gatggggttc gagacggcgc
    10621 aggggcccga cgtcgagacc gactactaca atttcgaggc gctcaatttc ccgcccgacc
    10681 accccgcccg cgatatgcag gacacgctct ttgtcgaagg ggggaggctg ttgcgcacgc
    10741 acacgacccc cgtgcaggcg cgcgtgctcg agacgcgccg cccgcccatc aagtgcatca
    10801 cgcccggccg gacgttccgc aacgaggcga tctccacccg ctcgcacgtc gctttccacc
    10861 aggtggacgg attcctcgtc gacgagggcg tgacgctcgc cgatctgaag ggcgccctcg
    10921 tcgctttctg ccgggcgttc tacgggccgg atatcaagct caagttccgc ccgtccttct
    10981 tccccttcac cgaaccctcg gccgaggtcg acatctcctg catcctctgc ggcgggacgg
    11041 gctgccagtt gtgcaagttc agcgggtggc tggagattct ggggtgcggg atgatcgacc
    11101 ccaacgtcct gaccaaggcc gggatcgaca gcgagaagta caccggttac gcgttcggga
    11161 tgggggtgga gcggcaggcg ctcctgaagt accggatcaa cgacatccgg ctgttcttca
    11221 acaatgacat ccgattcctt cggcaatttc gctagctcat ggaagtctcc taccagtggt
    11281 tgatcgagct gaccggggtg acctggtcgg ttgaggaaat ggcgcgccgc ctgacgctgt
    11341 gcgggacggc ctgcgagggc attaccccct tcgcccggca catggaccgg gtggtggtgg
    11401 gcgaggtgtg cgcggtcgcg ccgattgccg gcgcggacaa gctccggcgg gcgacggtgg
    11461 cgatcggggc ggagaccctg gacctggtct gcggggcccc caatgtcgcc gcgggacaga
    11521 aggtcccggt ggcgctgctc ggcgccaaac tggccgggga ggtcgaggtc cggcgcgcga
    11581 ccatccgcgg ggtcgagtcg cttggaatga tctgctcgga gcgggagctg gggctgtcgg
    11641 acgaccacag cggaatcatg gtgctcgaac ccgagatgcc cgtgggcgcg ccgctggccg
    11701 aggcgctgga cgcggccgac taccggatga cctttgagct gacgcccaac cgccctgact
    11761 cgatgtcggc catcgggatt gcacgcgaca tcgcggccct ggccagagtc cgcctgcggc
    11821 ggcccgcggt ccgcctccgg gaaacggcgg agaaggcggc cgatgtcatc agtgtgcggc
    11881 tcgatgatcc cgcggcctgt ccccgctacg ccgcgcgcgt gatcaggaac gtcaccctcg
    11941 gccggtcccc ttggtggatt cgccggcggc tcatgatgtc gggcgtccgc ccgatcaaca
    12001 acgtggtcga tgtcaccaac ctcgtcatgc tcgagtgcgg gcacccactc cacgccttcg
    12061 acctcgaccg cttcgggtcg cgcgaggtgg tggtgcggcg ggcgcgcgag ggcgagcggt
    12121 tcacgacgct cgacggcaag acccacgtgt gcgacccgca ggtgctgctg atcaccaacg
    12181 gcgcggtcgg cgtggccgct gcgggcgtca tgggcggcga aaactcggag gtggccgaaa
    12241 ccacccgcaa tatccttctg gaagcggcgt atttcgatcc gccgaccatc cgcaaaagcc
    12301 gccgcgcgct cgccacccag agcgaatcgt cgcagcgctt cgagaagggc gttgacccga
    12361 acgggatcga gtacgccatc gaccgggcgg cggctctcct ggccgacttg ggcggcggcg
    12421 aggtgctcgc cgggatggtc gatgcctacc cccggcggat cgacccgaaa gccatcgccc
    12481 tgcgcccccg ccggtgcaac gacgtgctcg ggaccgacct gaccgccgca cagatgaagg
    12541 agatcctgga ggggctggag ttcgccgtcg aggacggcga cccgttgcgg gtcacggtgc
    12601 cgacctttcg gccggatgtc gagcgcgaga ttgatctgat cgaagaagtc gtgaggattc
    12661 tcgggttcga ccgggtggaa gacgcccggt cgacgatcgg tccgctgttc acgccgctgc
    12721 atttcgagga caccttcaaa gaccaggtgc ggcaggtgct gacgggggcg ggattcgacg
    12781 aggtgctcag ccacggtctc gccgacagcc gcctggccga cctcgcggcg ccggagcttc
    12841 cgcagttgcg gctggtcagc acggccagtg cggaattgaa catcatgcgc aacagtatga
    12901 tcctctcggc tgtccccatc ctcggccata acgtggcccg gcgcaatgtc gacctgcgga
    12961 tttttgagat cggcaaagtg tacttcccgc cgaccccggc cggcgagtgg atcgaggacg
    13021 agcggatcct gctggcagtg accggcgata ccccgcacac ctggcgggag aagccgcgcc
    13081 cgcacgactt ctacgacgtg aaggccgggt tggagaggct ggcggaacac ttccactggc
    13141 cggcgctgga gttcgtcccg caggcggtat cgttcctgga gccgggagct tcgttccggc
    13201 tgcgggtgcg ggaaaccgag atcggggcca tcgggcagtt gtcggtgaag ctggcccggc
    13261 aggcggatat caagcaggcg gtatggctgg cggaggtggc gaccgggccg cttctgccgc
    13321 tttcctcgcg ggtggcggcg tacgagccgc tgccgattta tccggccgcc ccgcgcgacc
    13381 tcgccctggt cgttgacgaa acggtgccgg ccggcgatat tgtcggtgcg atccgcacgg
    13441 cggccgggga actggccgaa gacgtgagta tcttcgatct ctatacgggc aagcaaatcg
    13501 aggccggaaa aaaatcgatc gccgtggcga tctcctaccg ctccaaagaa cgcagcttgt
    13561 ccagcgagga agttgaagcg gcgcagcaga aggtggtcgc ggcggtgaag aaactgttta
    13621 acgcagaggt gcgggacaag tgaaccttga ggagaaattt cagaaattgc tggagcaggt
    13681 cggcggcctc accaggcaaa gccagaccct tcggaaagag aactccgacc tgagccggga
    13741 gaacgattca ttgaagcggg agctggccgg gttacgggcg gagtgccacc ggttaaaact
    13801 ccaggccgcc gaccgggcgg atctggttac ctccaagctg tccagcgtgt tacagcggct
    13861 ggacgaattg gagcgcaccg aggggcagta aattttttct tgacaggatt ctccgctcga
    13921 attataaatc gttgactgta agcgggataa gagagaacaa gcgcgatctg acaaaccgcc
    13981 cacaacggcg tttgaaagta tggcagcgaa cattgtcgtg gtgaatatat acggcgagga
    14041 ctacccgatc acgggtgtga ctgatgccgc tcatatttcc agggttgcag atttcgtcaa
    14101 tgcgcggatg cagcaaatcg cgcagagcag ccgggtgaag gctcgcgaca aagttgcgat
    14161 cctggcagcc atgcaaatcg catcggagct gctggagaaa tcagagcaac tggaatcgac
    14221 cctgggacag gtcgagggca acctcgattc cctgctggat cgcctcgatc gtgagctggc
    14281 gccggtttcc gacagtcgtt gagtggatca gcgaaactcc gatccgcgcc gttctcttct
    14341 tactcccttt ttgagataca tgcatcagtc caccggcacg cggtgccggg gtggaggacg
    14401 caatggataa aatcatcatc gttgccgccg ccgcggttgt cgccggcgtc atcggatact
    14461 tcctctcctg gcgtgcggcc cagcggatga atcgcgagaa gctcgcagcg gccacggtcg
    14521 aggcggagcg cgtggtcgag gaggccaaaa aggaagccga aatccggcgg aaagaggccc
    14581 gcctcgaggc ccgcgaggag ttcctcaagc tcaaagggga ctttgaacgg gaggtggccg
    14641 cccgacggca ggaactcgac gcagccgaca aacggctctc cgaactcgac accaacctgg
    14701 gacgcaagac cgagcacctc gacgccaaag aaaaagatct cgtcaaccgc gaaaaagcca
    14761 cccaggcgcg ggaaaagggg atcgctctgc gggaggaaga actcgaccag atcatcgccc
    14821 gacaaaatga acgcctccag aaaatcgcgc agatgacgcc cgaagaagcc aagaagcagt
    14881 tgatggacaa catgatcaac gccgcgcgca tggaggcggc ccaatacatc aaggaaatcc
    14941 gcgagaaggc cgaagccgac gccgaaaaag aagcccgcga aatcatcctc tcctccatct
    15001 accgctgcgc cgccgaccac accgtcgaaa ccaccgtctc cgtcgtcaac ctccccaacg
    15061 acgagatgaa ggggcggatc atcggccggg agggacggaa catccgctcc ttcgaaaccg
    15121 ccaccggaat cgacgtgatc gtcgacgaca cccccgaagc ggtgatcctc tcggggtacg
    15181 atccggtgcg gcgggagatc gcccgcatgg cgctggagaa gttgattgcc gacgggcgca
    15241 tccaccccac ccggatcgaa gaggtggtcg agaaggcgca gaaggaaatg gaagtgatcg
    15301 tccgggaggc gggggagcag gcctgtttcg agctgggcat tcacgggctg catccggaca
    15361 tcatcaagat gctcggcaaa ctgcacttcc gcacctccta cgggcagaac gtgctcgccc
    15421 acgccaagga ggtcgccatg ctgtgcggtc tgatggcggc cgaactggag ctcgatgcgc
    15481 cgctggccaa gcggtgcggg ctcctgcacg atatcggcaa ggcgatcgat cgggagacgg
    15541 aagggacgca cacgcagatc ggggcggatt ttatgcggcg gtataaagag gcgccggaag
    15601 taatcaacgc tatcgagtcg caccacggcg acgtgccgat ggtgggcccc tacccggtgc
    15661 tcgtgcaggc ggcggacgcg atctcggggg cgcggccggg cgcccggcgc gagcccctcg
    15721 aagcctacat caagcgcctc cagcagctcg aggaactggc cgacagtttc aagggggtgg
    15781 cgaaagccta cgcgatccag gcgggccgcg agatccgggt gatcgtggaa aacgataccc
    15841 tcgacgacct ggcgacctcg attcttgccg gcgacatcgc cgcccgcatc gagcgcgaaa
    15901 tgcagtaccc cgggcagata aaggtcacgg tggtgcggga aacacgggcg accgagttcg
    15961 cgcgctagag acgatacgcc cgcccggacg ggcggagatt attcgttgcg ctgaaggcgg
    16021 agttgttgta aactccgcct ttagtctgtg cggccggcag ggtggaagcg gtgggcgccc
    16081 cggccgcggg aaaacagtcg gcccgccctg gaaagttcac tcatgtcgtt agtcaccgtg
    16141 ttgtttatcg ccgatgtctg cggcaaagtc ggccgccagg cgctggcgca catggtcaaa
    16201 ccgctgcggc ggaagtacgg cgcgcactac gtcatcgcca actccgaaaa cgccgccggc
    16261 ggcttcggga tcacggccga gatggcgcgc aaggtgttcg cctacggcgt gaacgtgcag
    16321 acctcgggaa accacatctg ggaccggtac gacatcatcc ggtacctcaa cgagaacccc
    16381 cgcatgctcc ggccggcgaa tttccccgag ggcgtccccg gccgcgggtg ctcggtcgac
    16441 cgggtggacg gagtcaccat cgccgtgatc aacctgatgg gacggaccta catgaaggat
    16501 atcgactgcc ccttccaggt ggcccggcgg ctggtcgagg aggtgcggcg ggaaaccagc
    16561 gtgatcatcg tcgacatgca cgccgaagcc acctccgaaa agcaggccat gctctactgg
    16621 ctcgacggca aggtgtcggc ggtgatcggc acgcacaccc acgtggccac ggccgacgag
    16681 caggtgacgc cgaaagggac ggcctacatc accgatgtgg ggatgacggg gccctacgat
    16741 tcgatcatcg gcatggacaa agagccgtcg ctcgagcgct ttctcacggg gatgccgaag
    16801 cgcttcaaca cggccgaggg agactgccgg ctggccggcg ccgtcctcac gatcgaatcg
    16861 gagacggggc gggctcaccg gatcgagcgg atcatgtacc ccttcgacat cgccaccttc
    16921 cggcccaccg acatcgcgcc ggccgacggg acggaggacg cggcgccgga ggaggaattc
    16981 gacaatgaag ccgacgggga agaggtctga tgagcgcgaa gattatcgac ggaaaagaag
    17041 tcgcagccag cgtgctggac cgcctccagc cgcgcatcgc ggccctccgg gagcgggggg
    17101 tgacgcccgg actggcggcc gtcctcgtgg gcgaggatcc ggcctcggcg acctacgtgc
    17161 ggtcgaaagc caagaagtgc gaggagatcg gcctctactc cgaggtgatt cggcggccgg
    17221 cggatatcgg acaggacgag ctgatgcgca tcgtggccga tctcaacgcg aatccgaaaa
    17281 tccacggcgt gctcgtgcag tcgcccctgc cccggcacat cgatgagatg gcggtcaccc
    17341 tggcgattag accgggcaaa gacgtcgacg gctttcaccc ctacaacgtc gggatgatgg
    17401 ccctcggatt tccccggctg ctaccctgca cgccgcacgg catcgtcaag ctgctcgaat
    17461 tttacggcct cgatccggcg gggaaagaag tcgtggttgt cgggcggtcg aatattgtcg
    17521 gcaaaccggt ggccgcgatg ctgctgcaga aggcggcctg ggcgaatgcc acggtgacgg
    17581 tggcgcactc gcggaccgca aatctcgccg ccgtggtccg gcgcgccgac atcgtgatcg
    17641 ccgcgatcgg ccggccgcag actatcacgg gcgatatggt caagccgggg gcggtggtga
    17701 tcgatgtcgg cgtcaaccgg gtggaggacg aatcggccaa gaaggggtat cgcctggtcg
    17761 gggactgcga ctacgaagcc tgcgccgagc gggcctcgtg gattaccccg gtgccgggcg
    17821 gcgtcggccc gatgacaatc gcgatgctga tgtccaacac cgtggcggcg gccgaactcg
    17881 cactggtcgg cgcaaccgac tatgcaagca cctagctaaa gtgcgcaagc gcttgcgcat
    17941 cgtttcggtt ccgggcggac aggatggatc ttttggtcgg caatcgcccg cagcggacgt
    18001 tctcgctttc ctcacgattc tccaaccgat tccatgccaa tacgttaccg taccgtccgg
    18061 cccggcggag cgaactcgcg ggcatgtcag tctgcaacag cgatagcgaa gtttgagggc
    18121 atagactttg ctttttgcga cgctaggatt tctacacccg gtaggtaggt tccaacgcct
    18181 gaaacagatg aagttgacgc ttaagatact cctttctgtc ctcctcgcgg cccgtgcctt
    18241 cggcgccgtc gatctccagg caatcagtaa cacctatggt ccgttgctcc gcgagggccg
    18301 cctggcgcct ccgtcatctg cggtctatgt cgagttccgg gagtttgccg tggcgcttga
    18361 cgattccgtg gccgcggaca ccgcgcgcta cacgctcacc tgccgggtga ccgcaccgtg
    18421 gattctggtg aacagcctcg gcgggttcgt gttctcgttc cccgagggat tcagcctgca
    18481 ccacgcggaa gtgggggcgc tcaccgacga caacgccgga ctcaagttcg tcatccagga
    18541 aaccgtgatc gccgacaacg tcgtgacgat cgggctcggc gtctacggcc agcccggaga
    18601 gacggattcg cttgtaacgc tcactttggt gatagctgac gtaatcaatc cgcagacggc
    18661 cggctactat caggctgccg gtctggcgtt cgaccggcag gggatgattc tggccggccc
    18721 ggtgctgtca gatccattcc ggatctaccc gcgcgcgatt cagtcgctgg aggtgttccc
    18781 ggcggccgat acgacggtga ccgcggggac gctactggcg ttcggggcgg ccgggtacga
    18841 cgaattcggc aaccgggtcg atgatccccc ggtgagctgg tcgctcatgg aaggagcgat
    18901 cggaacggtg agcggcaata cgctggcgac acgcacggtc gggagtgggc gggtgcaggc
    18961 gacccttgga ggtctcacgg ccctctccgg cctgatcacc gtgattgccg ggccggccgc
    19021 cctgctggat tttgatgtcc ccccgcagca actggtgggg cagccgctct tcggtcccgc
    19081 ccgcgtgctc gtgttcgatg cttgcggcaa tctctgcggc gggcacgatc tcgcggccga
    19141 cccgttggag gtgatgggcg gggaaggagc gctggaaccg gcgtttctcg acgacaacgc
    19201 cttcatcagc ggcggcatca tcgatcttga cgcagccggt gtggtctatc ggggaccgac
    19261 ggcgcaaacg acgatctcgg gtcgaaccgc ctacgccgtg tccgacaccg agccggtgtc
    19321 gttcagcgga tatgactttc tcgcgctgct ggggccggac ggcgggacac cggcggcgct
    19381 cccggccgag tcgatcaccg cgctacgggt ggttgtctcc aaccgcgggc tcctggcgcc
    19441 ggcgggaccg gctgcgctgg aagcggattt cgcgagcagc ccggcgggcg gcggcacggc
    19501 cacttttgtt ggcggctccg acggccggac cgatacggtc ttgctgagca tctccaccgc
    19561 cggtctgccg gagggagaaa cggaattgac gctccgcctg gcggcggagt ttgagatcgg
    19621 cggccggatc tacccggtgg aacaaaccgc gttgtatccg gtgacgatca ctgcgcccat
    19681 cgctctggac ctggtgtccg agtctttggg gccggataca ctttatccgg aaatgcccct
    19741 ggaactcggg ttcgatgtgc gcgtgagcgg gttggcattg ccggtggact ccacgcgggt
    19801 taccattgcg ctggccggaa tcagccccgc gctgttctcc ggcatcgccg ccgcgacagc
    19861 cggcgaggac gggatcctgc agtatcgcgg tctgccggcc gcggctcccg cgatccctgc
    19921 ggcggccaca ccctacgagt tgaccgtcac gctgtacacc gacgggcaga tatatgaggc
    19981 ggcgttcacc gcgaataccg ccgtcgtgct ggagccggtg cggttgactt acgcgccggg
    20041 atccctccgc ccgcggttag tccccggcgg gtaccactcg gctttcatgt tcgatctcga
    20101 tctcgcggtc gcctacgacg tggagtggca gggcgtccaa tcggcgttcg gcctcaccgg
    20161 ggtcgatttc gcggcctttg ccagttttgc cccggtggac ggggcgccgg cgccgggact
    20221 taacccgatg gcgacggagg cgctgtttgt tcccgagagc caggtcggcg ggacgctggc
    20281 ttgcgcggga acggcggtcg tatatatccc atcggccggc gtgtccgaca cggtggcgct
    20341 cgatttcggc ggggaggggg tggaggtgcg gagtctcggg gtcatgcggg tggcggcgct
    20401 cgacgtgctg gcgccgaatg cccccaacgt gaacaccgat caggcgttcc ggctgctcgc
    20461 gcgggtggcc aatatctcga cgatcgacgt ggggccggtg acgctccgcc tgaccagcga
    20521 cggttactcg acctatgaca gcgcagaaac ggtcgcccac attccgccgg gagacacggc
    20581 ggatgtgtat ttcgactgcc gggcggcggc cgcgcccaat ccgggcgaaa tattccaggt
    20641 gcgggcggag ccggacacgc ccgaccaggc ggtcgtgcag ccgcccgatg ataacgtggc
    20701 gctggtgcgc attcagctac cggcggacct gcgcctgacc tacagcctct ggggcgtgct
    20761 cccggccgtc gtcgtggacc acagcacgcc cttcgggctc accgtggaga tcgaaaaccg
    20821 cggcgaggcg gctgtcgaca gcgggttcta ccgcttgctc accggcggca tcgatttcgg
    20881 cgccccggac acgctcgagg gacccatcgc ggtcggacgc cacatcgagt tccccttcac
    20941 cgcgccgtcc ttcgataccg cggccctgtt ccagttccgg ctcctgcgca ttccccatga
    21001 cctcaacctc ggggcgccgg cgcacatcga ggaggaccag ttctccttta tcctgcgtgt
    21061 cgagtcgcag gacgccgacc tgttcgtgaa agtcaccgag ctcggatcca acctgattag
    21121 tccgggtgaa gaccgggact tgttccgcat cgatctgacc aacgagggat cgtcgaccgt
    21181 ctccgtcatc cggctggagc ggatgcgcat ggatgtccgg gaccgcaccg ggcggccgac
    21241 cgatgccgcg cggctgttcg tcgccgaccg caccgttgtg ctcgaggacg gtgtgccggt
    21301 gccggccgaa gtgacggtag ccgaccagat cgcggttgct ttcgacggat tgactatcgc
    21361 ctcgggccac acccggtcac tgacgttccg gggcggattc gtcaacagcc tgccgccccc
    21421 cttcacgctg ggcctggaga ccgacgggtt cgaggccgtt ttccaggggg gaacgcacga
    21481 cggtttcccg gtgcgggtga aggggccggc cggaccgtac ctgttctcgc gcacttacgt
    21541 gatgcgcggg cgggggctcg aggaatccta cgtgattgag aacaacccgt tcaacccgaa
    21601 agtcggcccg gcgcgcttcg aactggaaat cgccgagcct tcggccgtgg aggtgcgggt
    21661 gtttacgctg accggcgaga cggtatacga ggacgaggtg ccgcaggggg acgtgcggat
    21721 cgagttcgca tgggacgggc gcaacggtga aggcgggatg gtgaacaacg gcgtatacat
    21781 cgtggcggtc aaggtggtgc ggaccggcga agaggcgcgc atgaaagtgg cggtggtgaa
    21841 gtagcatgat ggggcgccga tctctgagcg ggctggcggc ggcgctgctg ctggcgacgg
    21901 ccgcggcggt gactgccggg gacgccgggc gggagtcgcc cttcaccgtc ggcgtgggag
    21961 cgcgggcgct cggattgggc ggcgggttca cctcggtggc cgacgacgcg gcgaccatct
    22021 actacaaccc ggccggcctg acgcgcctgg actaccagga agtcacggcc atgcacatgt
    22081 cgctgctcga ggggacgatc tacgactacg cggcctgggt gtacccgacc gtgtcgcgcg
    22141 gggggttcgg gctcggcgtc atgcgcctgg gaacgggcga tatcgtgcgc cgcgaggagt
    22201 tcattgaaac cggcacgttt gactatgtgc aatcgcaaat tttgctgtcc tacgggcggc
    22261 ggttccaccc ccacttcgcc gccggggcct cgctcaaagt cgtgtaccag acgctcgacg
    22321 tctactcgaa ctggggagtg ggggcggatc tcgggctgct cgcccggccg cacgagcagg
    22381 tttcggtggg gatggtgctg cgggacgtga ttccggccgg cctcactctc aaccaggaag
    22441 aggaaaccgc gcccctgtcg gtggccggag gcatgtcggt cagcgggctg cggattcgcg
    22501 acgacatcgc ggggctgatc tcgttcgagc tggagcagat cgaggaccgg gcggtgcggg
    22561 tccacaccgg cggcgagctc ctgttcgccg gagcttatgc cgtgcgggcc ggctgggacc
    22621 gcgaccagtt ggctttcggt gccggcttcc gccgcgggcg gttccaggtc gactacacct
    22681 acaagctgat ggactacatc cccgactcgc accggttctc catctcctac gacatcgggc
    22741 cctcggtcgc cgagcaggct gaggaacagc gcctggcgga actacgcaaa ggcaccgatc
    22801 tgctcgagga agagcggcgg cggcagttca atctgtacaa gcagaaggcg gacgaattcc
    22861 gcgcccgctt ccggcttgat tccgccctga cctactacca gcgggcgctg gcgttcgacg
    22921 agaacaatca ggagattatc ggggcgatcg cggccatgga gagcacgcag cggatccagc
    22981 agagcgagca gacggatctg ctcgagcggc agcacgagtt gaaccggatg atggcgtcct
    23041 atctcgagca ggcgcggaat ttcgcgggga aagagtacta ccccgcggcc ttggacatga
    23101 tccagctgat tttcgaggtc gatccgaact acgcgccggc gcgggcgctc aaggcgggga
    23161 ttgaggcggc gatggcgact gcggtgtcgg aaactctcga aaccgcccgc gaggcgcggc
    23221 gcgacggcaa cacggtcgcc gcgatcgagg cctacgagcg cgtcctctac ctcgacccca
    23281 acaacgtgac ggcgcagcgg ggaaaagaag cgctggcccg gagtctcgat gtcgggcggc
    23341 acctcaacac cggcatcgac ctgtttaagg ccggccggta cggggatgcg cggcggcagt
    23401 tcgagctcgt gctctcggcc agtcccgacg aaccggtggc ccaggagtac ctgcgccgca
    23461 tcgagaccgc gcttgagcat ccgccgacgc tcgacgagat ccagcaggat cccgaggcct
    23521 ggcagctgta cctcgagggc ctgcgcttca tgcggaacca ggagtaccag aaagcgatcg
    23581 acgcgtggga gaaggtgctg gagcggtatc cgggcaacga agccacgctg gacaacattg
    23641 agcaggctcg actccgcctc aagtcgaaca accaggacta ggcggccatc ggctgccggg
    23701 gcggcacggc gcggcgcccc gacccgcggt cccgccggga cgcgcgtttt ttaaatggac
    23761 gccggcccgt ccatcgcata ctatcgacct atgttccgtc gtcccgtcaa agagatcaac
    23821 gccgaagtcg tggcggagga gaaatacctc gacagcctcc agcgcatcac ccgcgaggcc
    23881 tgcctctcgg ccgggctgtc caggaaagag gtctcgccca ttctgctggc gatcgaggag
    23941 ggggccacca acatcatccg ccacgcctac ctctacgaga aggggaccat ccggctccgc
    24001 atcgtgatct acaagaaaat gatcgtcttc tcgctcatcg acaccgggcg gtcgtaccag
    24061 ccggagtcgg acggcaagct cgacctgcag cgcctcgtcg aatcgggccg caaggggggg
    24121 ctggggttct acatgatcaa gaagatcatg gactcggtgg agtacatctc ctcggcgggg
    24181 tacaatgaac tgcggatgat caagcaggcg cagccggtgg ccagcgagag catccccttc
    24241 ctccgccaca tggccacgct gcgggcgaag ttttcggtct ggaccttcct catcgtgctg
    24301 gtcatcgttg ggggcgccta ctactacttc gacagccaga cgacgcggca gatgaaatcg
    24361 cacctcgacg agacggtggc gtcgctggtg aacacgatcg gggatcaggc cgccgggtac
    24421 gtgatcaacc ggcggtcgga cgtcgagttc gacgagttga tcgtgagcgc cgtgcgctcc
    24481 aacgccggca tcctgcggca ggtggtgctc acggacacgg ccggaacgat cctggcccac
    24541 tccgacgaca tccacaacat ccgcaaaccg tacgcgcccc cgccggaggt gcacgcggcc
    24601 cgcgtcgggg tgcccttccg gttctccgag aacgggcgtg cgctgagcta cctgatgctg
    24661 ccgatccggc agggggaccg gtcgttcggg acggtccacg ccgtgtattc ctccgagcag
    24721 ctccagcagc ggctggccga ggcgcgccgg cgcattctcg tcctcacttt cgtcctgctg
    24781 atggtggggg tggccgggat ctgggtcctg tcgaactact tcgtcgaccc gatcgtgcgc
    24841 atcacccagc gggtccggcg cttcgcctcg ggggacctgc gctcggaact gtcgctggag
    24901 ggcgcggagg agttctacga aatctcgcgc gccttcaacg acctcatgac ccgggtgagc
    24961 caggaccgcg aaaacatcgt ggcccgggag aagatggtca aagagatcga ggtggcctcg
    25021 cagatccaga agaccctcat gccgcgccgc ctgcccgatc tgccggggct ggaaatcgac
    25081 acgttctacc gggcggcggc cgtcgtcggg ggcgacctct acgacgtgtt cgagatcgac
    25141 ccgggcatgt actgcctggc ggtggccgac gtttcgggca agggggtgcc ggcctcgctg
    25201 gtgatgtcga tgctgcggac ggtgatccag atccaggcgg ggcagtcgca ctcctcgcgg
    25261 cgcaccctcg tcctcgtcca cgagtacctg cgggagaaca tcccgcccgg gatgttcatc
    25321 acgatcatgc tggcggtgta cgacagcgcg cggcggaggc tgaatttcgt gtcggccggc
    25381 cacaaccccc tcctgtacta ccacgcggcg agcggggaaa tccgccccct caacccggcc
    25441 ggaatgccca tgggcgtgcc ggccgcgatc gacacgacct tcgagcagcg cctcgagcag
    25501 tacgagctcg agctgcagga cggggacttg tttttcatgt ttaccgacgg gatcacggag
    25561 gcgtcgagcc gggaggggca gtactacggc atcgaccggc tcatccggtt catgaaggcg
    25621 caattggccc agcccgacgt gaaactgccg ggcatctcgg cggccctcat ggccgagctc
    25681 gaggagttct gcgggttgtc ggggccggcc gacgatatca ccttcatcat cgggcgggcg
    25741 gcgctgggcc gcctcgcgga cccgcccgag gggacggccg acggggcgcg ggagagaccg
    25801 tcgaacccga cggcggccgg cgggccgaac tgaccggccg tgttttatgc ttgcctgcat
    25861 cgggcggaat cctttccttg aggccgactg aacccaggca gctccggctg cagaaacggc
    25921 catggaaaat atctccattt cgctctccga gagcgagcgc gacaacattt ctgagatccg
    25981 ggtcgacggc gtgatcgaca cgacgacggc gggggagctc gaagaggtca tggatgcgct
    26041 gatgaagcgg cggcgctacc gggtcgtggt cgatctggcc ggcgtcgact atatctcctc
    26101 ggcgggctgg ggcatcttca tctcccacat caaggacgtg cggaacctcg gcggcgacat
    26161 caagctcgcg aatatggtgc cgaacgtcta cgagatctac gagctgctgg agttcgacag
    26221 agtcctccgg gcgcacgaga gcgtggatgc ggcgcggcgg gagttcgggg ccgcggagtc
    26281 cggagaggca aaaaaaaaag cggagtgacc gccggactga cggtggttga cgaccttggt
    26341 gttgagacgg ccgaccgctg ggggccgcgc gggatcgggc cggccgacga ggtgcttaac
    26401 ggcggggatc tggacaccgc ggtgcttcgc ctggtcaagg atgacccctt cgcgagtatt
    26461 tccgaactca gccgcgccgt ccgtgaaacg ggtctgttcc cggatgccgg gtggtggcga
    26521 atcttcggcg ttctccgccg gcatggcttg ctgagccgcc gctcgcggtt tcgctttggg
    26581 cgcgggcgcg ggaggggtcc ggcccggcgg ttctgagccc cggcttcccc gcaggttctt
    26641 ctttggcaga gagtaagcgg aatggatgac cagacggtcc tgacggttgc actggcgctg
    26701 ctggtggtcg tgctggccgc cctcctggtc caccggattc gccgggagcg gcgccaggtg
    26761 gaaggcctgc ggcgcatgaa actcgaggaa ttcagcgagt tcctccgccg caacagcgtc
    26821 gagggcaaca tcgtcgaagt ggcgcggaaa gtgtcggatc tgctgcgcac ggcgttcggg
    26881 tgcgacctga tcctgttcct ccgcaagaaa cgggatttcc tggagctcag ctaccaccac
    26941 gggttgcggg acatcgaccg ggcgcagctc cgcgcggcgg ccgaacgggg cctgctggct
    27001 gcgctggcga ccgactttcg ccccgcgccg ctcgatcggc tgcaggggca tttcgccgac
    27061 gattaccttg agcggctgcg gacccacggt ttcgggctct atttccccgt cttctggcgg
    27121 gacaacctct acgggctgta cttcgtgcgg agcacgctgg agacccaatc gccgtcgttt
    27181 cacgtgctgg tggcgagcct ggcccactcg ctcgcggcgg cctaccacat caagtggcat
    27241 gagtcgcgct acgacctggt gcgcgagcag ctcgagcggg cgcgggcctc ggcacaaaaa
    27301 ccgcctgcag gggaagggcg cgccgccagc cacctgctgc ggctggtcac gcaccgcaat
    27361 tcgcagacgc ttctgccgaa actggtggag tcgatccgca aggatctcgg actcgagcgg
    27421 gtggcctacc tctatcagcc caacggcggg ggggaaccgc aggtggtgca ggacggcgtc
    27481 cggatgaacc tgccggcgca ggaaaagggg caggtcgagg gggtcctgcg ggcgatcaag
    27541 aatgaggacg tctacacgct cgcgcggctg gcccaggagc agcccgggct gcgggagtgg
    27601 gtcaagagcc tccgcgaggc cgggctcgac cacgtgacgg ccttcccggc gatacagggg
    27661 cggccggctc tgctggccta cagcgaccgg accggggcgg tgccccggga gcaaatcgac
    27721 actgtgcgcg gccacgtccg cgacctgatc gccaacgtcg aaacatacga gaagatcgag
    27781 gagctgtcgc agacggacgc gctcaccggg ctggccaacc agaggtattt caagcgccgg
    27841 ctggaggagg aggtgcagcg cgccaagcgc tacggccgcc ggctggccct cattttcttc
    27901 gacctcgacg gtctgaaaca gaccaacgac acgtacgggc acctggccgg cgattcgctc
    27961 atacggcaga tggggcggat cctgcgcaac aacatccgat cgatcgacat catcgcccgc
    28021 tacggcgggg acgagttctg catcatcatg cccgaggccg accgcgctat gtccatccgg
    28081 ttcatggagc gggtcaagat cgaggtggcc aaagcggagt tcccggtgac cggtttggca
    28141 gcgcccctga cttcgacagc ctcgctcgga ggagcgctct accccgacca cgccgacaac
    28201 ccgcgccagt tgatcttcgc cgccgacatg gcgctcctga aggccaagga acaagggcgg
    28261 aacaaagcgg ttctgtatgg ggacgcgccg gccgagtcac cgtgaatctc cagccggcgg
    28321 gaagcatcgc ggcctccgta gcgcaaacgc agatatgcct ttacagagat gttggaagag
    28381 tgtattttga tttaatggag tctcggcgac gcgccgatat gtatataaga cattagatgc
    28441 cggatactta cagggaggtt ggcacaaaaa gtatgaacac gagattgttg ttgctctcat
    28501 cgctgctcgt cctgaccgcc tgcgggatcg gctccggcca ggaataccgc atcggtcccg
    28561 gggacgtcct cgagatcgaa ttctggcagg atccgaccct gaattcgacg ctgcgggtgt
    28621 cctttgacgg gacgatcacg ctggacatca tcggggagat caaagcctcg ggccggacga
    28681 cgcaggagct gcaggaggac atcgtgcgcc agatgtcgcg cctgaaccag cggatttcgc
    28741 aggcggttgt ccgcgtgcag gagtataact acctctacgt gttcgtgtcc ggacaggcga
    28801 accatccggg caagatgacc tttgaagaga ttccggattt gtggacgatc ataaatgagg
    28861 cgggcggtgt aaccgagacc ggggacctga gccgggtgac gattatccgc ggcggcgagg
    28921 aggccggccg cgtcgaggtc gtgaacgtgg ccgccgcgat cgccgcgggc cagctcgggg
    28981 ggctgccgaa gatccggcgg ggcgacacga tcgacatccc ccgcacgccg gtggggctgc
    29041 cctcggcgag cttcgaccgg tcggccgagc gcaaaaacct ggtctacgta gtcggcgcag
    29101 tgaccacgcc cggcccggtc gagtaccagc cgaacatcga cgtgctcgag gcggttgcgc
    29161 tggccggagg cccgaccaac gaagcgaacg tgaagaaggc gcgggtggtg tgcaaggacg
    29221 agttctacgg ccaggcgctc gagatcgacc tcgagcgcta cagccgctcc ggcgcccccg
    29281 cccgctacgt cctcggccgc gaggatgtcg tggtcgtgcc gcacaagagc ggattcttcc
    29341 aggggttcaa cgtcaccacg gtagcgaccc tggccggcgt gatcaccacc tcgatcctcg
    29401 tgtacgatca attgcgccgg gatgactaaa ccgggacagc catgcagaca ccgcaggaaa
    29461 aaccgctcaa tctcaaggac atcgccgcca ttctcctgaa gcggaagtgg ctggtgatca
    29521 tccccatcat cgccgtcacc ggtctggcct tcgccagcac ctacctgatg acgcccgtgt
    29581 accggtcctc gaccatcatc tggatcgacc agccgtcgaa cgtgtcccgc gaactcggga
    29641 tgatcctcgg gcgcgaacag gagggacgag tgagcagcga ggagcagcgg cggcaggtcc
    29701 aggcgctgca gaacgagctg acctcgcaga cgtacctctt ccagctgatc aaagagctcg
    29761 ggctcgacaa cgacccggag gtcacgcgcc aggcggccaa gatgcgtgag gcgaacctct
    29821 cctactccct cgatcagttg aagctcaacc tgctggtgga cgacatccgc gggcgggtcg
    29881 aggtgtcgtt cgtcggcaag gaccagatcg agttggcggt caagtcctcc gacccggtgc
    29941 aggcgaaaga tatggtcgtg agcctggcgc ggatcatgga acaagagcgc accaagtacg
    30001 aactcgagaa gattctcgac aaccaggatt tcgccgacct gcagctccag aagacggagt
    30061 tccgctaccg gcagatgctg gactcgctga ccgcggccca ggcgcgcctg cagtcgctgc
    30121 agcttcccga gaacatctcc gccgaggcca accgccgcga catcgtcacc gacatcgaca
    30181 agacccagct ggagatcgac gactactcgg atgagcgcga cgagctccgc gggcgcctgg
    30241 ccgaactcgc cctggcgtcg gcccgcctga aatacaccga ttccatcgtg gagctgcgga
    30301 cggagatcga cgggcagatg acccggtttg ccaccatgat ggagaagtac gcctggaacg
    30361 agcagaacgt catcaacgtc aacgtccgcc tcaacgacaa cctccggcgg ctcgaggggg
    30421 cgatccagca ggcggtcgac aaccagtttg cctcctatga ggcgagccaa cgggatctgc
    30481 tgaaacggta tttcatcgcc gaggagcatc ttgacgtggc gcggtcgcgc aaatcccagc
    30541 tcgaaacctc gctggccaac atcgatcagc gggtcaaccg cctcccccgc cttcaggccg
    30601 aaattcagga gctcgagcga cgcgtggatg atgcccggaa gtatcgggac gccttcaagt
    30661 cggaggagac cacggtcggc attctgtcgg agcgaaccaa ggagcggacg acctaccggg
    30721 tgatcgagcc ggcgcgggtg ccggtggcgc cggagtggcc gaaccgcaaa aagatcatgg
    30781 tgttcggggt ggtgctcggc ttcctcctcg gcggggcggc cgtgtttctg gccgagatat
    30841 tcgatacctc ggtcaagcgg atcgaggata tcgaggagcg cctcgggctg cccgtgctcg
    30901 ccaccatccc gcgcatcgac aagttgagga gcgtcaggcg ctagaccatg gaaacatatc
    30961 gtctcaagag cagactggtc gaccacgacc gcgagttcgt catccagacg gccaacgacg
    31021 ccaacctcgg cgccatttcg tcggaaatct acatcgacgg ccggctggcc gaggccgcca
    31081 gccaacccca cccgcccgac atccgggccg aggaagtcct ctcgctgctg aaagaaaccc
    31141 acggcgagcg gaagaaggag ctcgagacgc tcctgggggc ctaccgccgg atcatgaacg
    31201 aggggaatcc ggagatgatg ctgcacctgg ggacggcgct ctttcacagg aatttctttg
    31261 ccgacgcgcg gagcgtcttt caggcggcgg tggcgctgga tgcgaaaaac cacctggccc
    31321 gcgcctacct cggcatgacc tgcctggcgc tcgacgaggt cgatgaggcg gtggcggccg
    31381 ccgaagccgc ggccacggcc cggccgggct acgccgacta tcgcaacggc tgcggcgaag
    31441 cgctcctggc cgcgggccag ttcgagcgcg cccaccgcga gttctccgaa gccgtcaaga
    31501 tcaacctcta ctacggcgac gcctacttca atctcggcct cacctacgtc gggctggcca
    31561 gcaccaacga gatggtcaag gactcgcgcg cgctgttgaa caaggcggcc gattgtttcc
    31621 gccgcgccgc catggtcaac gtcgggtacg acgccggcta tttcgacgac ggcatgcggg
    31681 cgctggcggc gggcgaggcg acccgggcgt gggcggcgat gaacgaagtg aagtccgcgt
    31741 ggaaggcccg gcggcggcgc gagctggccg gattccacat gcgctacgtg ctccacccgg
    31801 aatgggtgtc cgagcagatg atcgaggagc ggatccgcta cctcgagggg gaggtgcggc
    31861 gcaatccgaa ttacgtggac ctctacacgg aactgggccg ctgctacctg gagcgggcgc
    31921 ggctggggtg gcagaagggg atcgcgcagt accggcgggc cacggaactc aatcccgccc
    31981 tgacccaggc ggtcgagcgg ctgcgagcgg cggaagacga atacgagcgg atgcgctcca
    32041 cgctggaagc gatggggccg aaaggataat caccacatgg cgcggaaacc cctgtccatc
    32101 atcgacctgt tttctctcga ccagcccttc gccacggagt tccgccggct cctccaccgg
    32161 ctgcgcaagc acggcgagag ccgcgagacg aaagcgatca tgatcacctc ggccatgctc
    32221 gcggaaggga aatcgacgat cgcggccctg ctcgcgctca ccgcggcgcg ccagaagggg
    32281 ctgaaaaccc tgctcatcga cagcgatctg cggcggccct cgatccacaa gttcttctcc
    32341 accgagcggg ccccgggact ggccgacacc ctcggcaacg gggtggcggc ggccgacgta
    32401 atccggaaaa ccgcgttgga gaacctgcac ctgatcaccg ccggacgggc cacgcctcag
    32461 ccggccgagg tgttcgacgc ggaggcgatc ggcacgatca tcgaggaaat gaaattcttc
    32521 tacgacctca tcatcgtcga cgccgccccg ctcctgccgg tgtccgatcc gctcctgctg
    32581 gcggccaaag tcgacggcct cctcctggtg gtcaaggccg ggtccacgca gcgcgaggtg
    32641 gtggaacggg cggtcgacat gctcgaactc caccgcgacc gcgtgctcgg cgtgatcctc
    32701 aacaacgtca acaacagcct tccctactac tacgactatc gctactacgg ctaccaatac
    32761 ccgacagaca agccgacgga ggggtcggcc gaggcgggcc gcaggcactg gcggcgcggc
    32821 agccggggcg ccgccggccc ctcgaaagac aatccgatca agcaacagca ctgagtccgc
    32881 gcccctatgg aatcgtcgat gaacattctg accgtggacc tggaggagtg gtttgtcgtc
    32941 gaggccctgg ccgagcggct ggaatcgcgc cagtgggacg ggctggagtc gacggtggcc
    33001 aagaactgcc accgcctcct ggagctcttc gggcggcacc gggtctcggc gacctggttc
    33061 gtgctggggt gggtggccca gcgccacccg gagctgattg ccgagatcga ggcggagggg
    33121 cacgagatcg cctgccacag ctactaccac cggcgggtgg atacgttgac gcccgaggaa
    33181 ttccgggccg acaccctccg ggcgctggag gctatcactg ccgccgtgca gcgccgcccc
    33241 cgcggctacc gggcgccgag ctggtcgatc aacgacagca tcccgtgggc gttcagcatc
    33301 ctggcggaac tggaattcga ctacgacagc tcgatttacc ccatcaagca cgacatttac
    33361 gggatgccgc acgggccgcg ggtgatccac cgtatgcacc tggaggacga ccggctcctg
    33421 tatgaagtgc cggcctcgac ctaccgcctt ctcggatcaa acatccccat cggcggcggc
    33481 gggtttctcc ggcattcccc ctactggtac tcgaaacaca tcatccggcg gctgaacacc
    33541 tcgggagtgc cggcggtcgt ctacatgcac ccctgggaga tcgaccccga cccccccaaa
    33601 ctggccgggt tgacggcgat gcagcggctg cgcatgtacg gctcgacctc gattttctac
    33661 cacaagctcg atcgcctgct cagcgatttc gagttctgcg ctctggccga ttacctgcgc
    33721 cttctgaagc ggcggcagat cggatttctc gaacgcccgt agccggcggg ggcgggagcg
    33781 cgacgttttc cgcaaaatat ccttgactaa atgtgtataa gagtaaacct tggttgggag
    33841 accggagaac caagaggagg ttaatgacaa atgcttaaac gactgctact tgcggttctt
    33901 gctctggctg tttgcgtgat cccggcggaa ggcgccaaga agaagccgaa agcgggggcg
    33961 atcaaggacg gctggtatgt cgacgcgaaa tatgacttca aattccaggt gccgggaaac
    34021 tggagcgtgg cgatcaagga cggggacgac ccggtgcggg cggtcctcgt gcagaaaaac
    34081 taccagatcc ctccggagta cctcgatgcc gaggactaca ctgaagtgcc tcgcctgacg
    34141 gtctatgttc tgcagacgac gatgagcgcc ctggagttcc gcgattcgct gttgtcgaag
    34201 acctacgaat cggaccagaa gaaggatatc cggaagtact ttgagatcct caatgacgtg
    34261 tcgatcggcg aggggacgcg gcgggagccg gtggtgacca agtcgcgcga gctcttgtat
    34321 ctcgactccc taccggcgtc gctctggttc ggtgaggcgc agtacgtgaa gaacgtcacc
    34381 ctctctgcgg ccgcgcagag cggcaagcgg gtgaacggcg attacgccgg gaccatcgtg
    34441 gccgtcaaaa acggcgacaa cctgctgctg ttccacctga tgagcgaagg tatgtaccac
    34501 tcctcggtgt ggcaggagat tcggaccatg atcgaggggc tgaagtggcc gcgctgagcg
    34561 ggcagcgtgc tacagtttgt tcgggaaagg ccggcgggga ccggcctttc ttttatgttg
    34621 accgggcgct tccgcagtcc tatcatgtaa agctgaggtt tcagcgagcg atggaggtcg
    34681 tatgtgggcc gtcagggccg tgctcatcac cgtgatcgtc atcgcggtcg tcgcgtttgc
    34741 gatccataac gtcggcgaat accagaaggt cgacgtcaac ctgatttgga agagctacca
    34801 ccaggttgcg ctggtggaag tcgtgttctg gtcgtttgtc gccggcatcg ccgtgtcgct
    34861 cgtgatgttc gtgtcggtgt acatccgcct gtccatggcc gggcgggcga tgcgcaagca
    34921 ggtgcgggcc ctggaggccg agctggccgt gctgcgcaac cggccgatcg aggagtcggc
    34981 gcaggtgctg gacgaggacg ttccgggccg gcgggacctc acgtcgccct ttgacgagcg
    35041 ggagtagcta tggccgaata cgtgctggca tttctcattc tcctggccgc ctcaatcatc
    35101 ttctacctcg gctacgaccg gctggtgcgc cgaacgccca tgccgtcgac cgccctctac
    35161 gtggaggccc tccgggacct cctcgacggc cagcgggtgc gggctttcgg aaaactgcgc
    35221 caggttgtca acgaggacta ccacaacatc gacgcctaca tccggctggg gcagatcctc
    35281 cgcgagggca acaagccgga ccgggccctc cagatccaca aggatctgac gctccgggcg
    35341 ggcctgccgc cctcccagaa gatgggcatc ctccaccagc tggccgagga ctactccgag
    35401 ctgaaggatt atgacatggc cgccgcggcc atcaaggaac tcatctcgct ggacggcaac
    35461 aaccggtggg cgcatatcaa gctgctgaag atccaggaag cgaagcagag ctgggacgag
    35521 gccttcgaca cggcggccca catcctcaaa ctcgaaaaca acaagtcgaa aaaaccgctc
    35581 gccttctaca agtaccagca ggggatgcag ctgtacaaac agagggaata ccacaaggcg
    35641 cggattctct tcaaggaagc gatcgggctg aaccccgcct acgcgccggc ctacctggcg
    35701 gtcggtgatt cctaccggct ggaggagcgg cacgaggacg cggtcaattt ctgggtcaag
    35761 ctgatcgaag cggtgccgga caaggggcat ctcgtgatcg accggctgaa aaaggcgctc
    35821 tacgatctgg gccggttcag cgaactggcc gacatctgcc acaacatcct ggcccacgac
    35881 tcgggcaatg tcgaggcgcg gctgtcgctc gccgagttct accacgcgaa aggggaaatc
    35941 gaccaggccg agacgctcct ggccgaggtc gtggagcgtt cgccggaaaa cctcaaagcc
    36001 atcatcggct atctccggtt gctcgtcgaa aagggcaacc ggcggcagat cgaccggttt
    36061 tttcgccagc tcgaagggcg gctggagaag ccggcggccg acgcccgcac ggccccgggc
    36121 ggcaagatga tcgggaccgc ctgatccgaa cgggaatccg cccgcgccac cggtcatgaa
    36181 aaaactgctc tgcatggtca tgctggccgc cgccccggcc gccccggcct ccgccgacga
    36241 cctcatctac cgcctgatta cccaggggaa actcgatgag gcgcggcagg aactgtcgcg
    36301 ggtgtcgacg gcgtcggtgc gcgacggcgc cacgctgttc ttccaggcgc tcctggagcc
    36361 cgatgccgta cgcgcggccg agctgttgga ggtggcgctc ggtgcgggcg tcggggcgga
    36421 gtaccgcgaa ctggccaatt tgcggctggc ccagtactac tccctgacga aggcctggga
    36481 caagctcgag cgcgtgattg tcaactaccg cgcgaagttc gagcacggcc gctaccgcga
    36541 acagatgatg cgattctcca cgctgctgga cgagcaggcc ggggccggcg aatcggccct
    36601 ccgccaggcc gaccggtacc tggtcgagta cggatcgggc ttggcgcagc agtgggggca
    36661 aatcgacaag gcgcgggtgc tccaggggag caagaaggaa atcggcgccg ataagatgct
    36721 ccgcggactg gcgcgcgaaa agaccggacc aggcgtcccc caggccctat acctgctggc
    36781 gatggatgcc gcaaggcggg gaaaggccga cgacgccgtc ttctactaca acgtgctgcg
    36841 ggaggcgtac ccgaacgcgg tcgggctcga ggcgctggtg gatcggctgg cagtcatggc
    36901 cgaaggagtc gagcgccgcg ccgccgaggc cgaacaggtg accggcacgt actacaccat
    36961 tcaggtgggg gtgttcaccg atgccgacaa cgcccgcacc caggccgagc gcttccggca
    37021 gaccggctgg aagaccgaga tcgccgacaa gaccatctcc cgcgtgacct accgggcggt
    37081 gtatgtcggg cggttcgccg cctaccagga ggcggccgcc gccaaagccc gcctcgaacg
    37141 cgagatgaac gaaacctacc aggtcgtcgc gcgatgacgg gcccgcggga cacagacgat
    37201 tcccttactc ccctcatgcg ccagtactac gcggtgaagg cgcagcatcc gggcaagatc
    37261 ctgtttttcc gcatggggga tttctatgag atgttcggcg acgacgcggt gaaggcagcc
    37321 cccattctgg ggattgcgct gacctcgcgg tcccacggcg ccaacgagcg cgtgccgctt
    37381 gccggcgtgc cttaccattc ggccgacaag tacctcgccc gcctgctcgc ggcgggggag
    37441 aaggtggtgg tggtcgagca ggtcgaggac cccaggaccg ccaaagggct ggtcaaacgc
    37501 gagatcgtgg aaatcctgac gccggggacg gcgacgatcg agggagaggc ctcggaccgg
    37561 caggcggtgt ggctcgcggc cgtctgcccg cggggagaca gcgcgctcgg gctggctgcg
    37621 ctcgacctgt caaccggggc gttcctcgtc gacgaggggc cgaccgacga ggtgcgcgag
    37681 cgcctgcgcg tcctcgaacc gcgcgaggtt ctgtacccca ccggcgggga agcggaggac
    37741 ggccggcccg ggttcgatcc ggccaatggc gcgatccacc tgaccgggtt cgcggagtgg
    37801 aatttcgact accggacggc ggtgcgggag ctcagcgagc attttgggac gagcacgctc
    37861 gacggattcg ggatcggcga ggcgcgcctg gggattgcgg ccgcgggggc ggtgttccgc
    37921 tacctccgcg aaaaccaccg cgagcggttg tcgcacatcg accgcatcgt gcggttcgac
    37981 accgcggagt acatggtgct ggactacggg acggtgcgga atctggagct gctgcgcaat
    38041 ctcgccgagg ggaccgagcc gaattcgctc ttcgccgcgg tgaacaggtg ccggacggcc
    38101 ccgggggcgc ggcggctggc ggccgctctc gtgcggccct tccgcaacaa agcgcggatc
    38161 gagaagcgcc tctcgggcgt ggccgaactg gtgcgcgacc gggacctcgc cgcccgcgtg
    38221 cgcgaacata cccagcggct gccggacctg gagaagatcg ccgggcggct ggggatcggg
    38281 aagctgaacc cgcgccagat gaaagcgatc ggcgagggac tggccgagtg ccgcgcgctc
    38341 ggccgcattg ggctggtaga ccgcctggtc cagcgcgtc
//
LOCUS       DAOPOE010000017        37581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1472_length_37581_cov_5.523637, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000017 DAOPOE010000000
VERSION     DAOPOE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_37581_cov_5.523637"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..3259)
                     /gene="secA"
                     /locus_tag="PKM94_04025"
     CDS             complement(218..3259)
                     /gene="secA"
                     /locus_tag="PKM94_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOD65838.1"
                     /translation="MANLLTRIFGSKHDRDIKKIAPLVAEINEHYERLSVLTEEELTG
                     KTAEFRQRLAEGATLDDLLPEAFAVVKEACRRHVGKTLEVVGLPVEWNMVPFDVQLIG
                     GIVLHQGKIAEMATGEGKTLVATLPTYLNALAGKGVHIVTVNDYLARRDSEWMGWIYT
                     YLGLTVGCIQNQMTNAERRTQYLCDITFGTANEFGFDYLRDNMAQRVEDRVHRNFGYA
                     IIDEVDSILIDEARTPLIISGQVETTTVHDRYREMLATVETLVRKQTRLINDMVAEAE
                     EILAAEDGDHFQAGTLLLAARRGAPKNKRLLKVEKEPGVAKLITDVESAYMRDKKLHE
                     IDDRLYFFIDEREHTIALTDLGTAQLTKEEQNLFELPDLSSMLSEIEGDQSLAPEERQ
                     KRIDAAYAVHAERSEKVHAINQLLRAYSLYEKDVEYVLQDGKVMIVDQFTGRILSGRR
                     YSDGLHQAIEAKEGVKIEAETQTLATITLQNYFRMYEKLAGMTGTAETEAAEFWDIYK
                     LDVTVIPTNKPVIRKDMDDQIFRTRREKYNAIIEEIQTQYNAGRPALVGTTSVEVSET
                     LSRMLKRTGIPHNVLNAKQHQREAEIVAQAGQRSAVTIATNMAGRGTDIRLGPGVVEL
                     GGLHIIGTERHEARRIDRQLRGRAGRQGDPGSSLFFLSLEDDLMRLFGGDRLGAIMDR
                     LGVQEGEVITHGMVTRAIERAQKKVEAQNFAIRKHTLEYDDVMNVQRKWIYERRLAAL
                     ERPSIQEEVVELIEGVLDDLLAEYAPEKDHPEEWNWTGLRDKLREIYLLNFEVRTDDI
                     AEFTQAKLREQLLGAVMAFYRQKEAAYGPEIMRQLERYAVLSTIDRHWRDHLAEMDEL
                     RTGIGLRAYHQGMGKPIDIYKREAFKMFEDMVVRIDREAVNLVYKLRVNIPEQSRTDR
                     RRVPQNLQTSHADATNMGLGGGRAPGEPVGAGMRGAGPTGAGAGPAGMAPHERDPMAE
                     ASQAGRQARTIKREMPKVGRNDPCPCGSGKKYKKCHGAGEA"
     gene            3494..4690
                     /locus_tag="PKM94_04030"
     CDS             3494..4690
                     /locus_tag="PKM94_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD65839.1"
                     /translation="MTAPTDRPAVPRIPPGVWVLGFVSMLMDVSSEMIHGLLPLFLTT
                     VLGAGVIAVGLIEGLAEATALIVRVFSGIVSDYLGKRKALALLGYGLGALTKPLFALA
                     PHAGVVLAARLADRVGKGIRGAPRDALVADITPPALRGAAFGLRQSLDTVGAFLGPLA
                     AVGLMLLWHDNFRAVFGLAVIPGAAAVVLLALGLREPGRPPAGRRTNPIRRDNLRLLG
                     PAYWWVAAIGAVFTLARFSEAFLVLRAQQAGMAIALMPLVMVVMNAAYALTAYPFGRL
                     SDRLSHTALLAAGLVVLIAADLVLAAGSGWRLVLVGVTLWGLHMGLTQGLLARMVADT
                     APATLRGTAFGFFNLISGLAMLPASVVAGLMWARFGAAATFYTGAGFGALALAGLLAR
                     RPARSA"
     gene            complement(4718..5971)
                     /locus_tag="PKM94_04035"
     CDS             complement(4718..5971)
                     /locus_tag="PKM94_04035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD65840.1"
                     /translation="MSGSEWSYDQVLRGDPRACAARPWDAVIIGAGPAGSVCARQLAH
                     SGHNVLVIDKERFPRHKVCGDLLVPDALAMLRRVDLHARIAALAHASRTIEVASPSGI
                     RFAVPGEYLALSRKRLDYELCRHAAAAGAHVLAGNAAGVEPAAGDGLVRVHLSGVAEP
                     ITARFAAVATGAVVDLAQRIGLVTDPEPSAIALRCYVRSPRRRDDTIISYDESILPGY
                     GWIVPLGEDHGHPGAFLYNVGCGTMYRHVRDGRHMLKRTLHRFMERFPPAREIMAGAE
                     IVSPIAGAALRCGLRGLATAHCGNVLAIGETIGATYPFTGEGIGKAMETGSLAAETLH
                     EALAADDPSLLGRFSERLRELRPRYRGYAVAERYLGRRRLNDYVARRITRSRYLQRQV
                     EAFMAETESPRRLFHPLSIILSYFC"
     gene            6095..7003
                     /gene="rsgA"
                     /locus_tag="PKM94_04040"
     CDS             6095..7003
                     /gene="rsgA"
                     /locus_tag="PKM94_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOD65841.1"
                     /translation="MTEREKGIVVATRGRLFEVCAADGARVLCEVRRKVKHQTEHETP
                     VAVGDDVVITRVSPGGGAIDEVLPRRTAFVRPAKGRETKKQVLAANLDRLAAVVSIQA
                     PPLRPGLIDRFLIAARLGGLEPLVVINKVDLEPPPGLDDVVAVYRSLGCGVFLVSGLA
                     GTGLGELCEALRGHRTLFAGHSGVGKTTLLNAFVPGLRLRVGQVSPITRRGRHTTTTI
                     EMCELPTGGFVVDSPGLRVLGLWEITPDDLRGMYHEFEPFAGECRYQPCSHTHEPGCA
                     VKKAVAAEKIHALRYENYLAIAASLP"
     gene            7069..7971
                     /locus_tag="PKM94_04045"
     CDS             7069..7971
                     /locus_tag="PKM94_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD65842.1"
                     /translation="MAAKNIEFRVGVIILIGLVVLGASLYWLQGYKLERNAQVIQVLF
                     DDVGTLSVGDRVTVSGVHRGKVDRLDLVDDGVLVKILLYRDVVLHRDARFIIRNMGVM
                     GERFIAITPGKEGAPFDFDSVAVGEYDTGLPEVMGELGEMVIEVRNLVGSFRRNLGSD
                     STLEALNQTVRNLEDVSGSMAEFLDANQSGLNSAIRNFQSAAGRLNELVTRNADQVDS
                     TLATVNRTSARLEEFTYRLDTLAGAARQFADMLNNPDGTAQLLLQDRRLYDDLRSTAD
                     NIDDLVKDIRANPRKYLDLKVEIF"
     gene            7977..10136
                     /locus_tag="PKM94_04050"
     CDS             7977..10136
                     /locus_tag="PKM94_04050"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOD65843.1"
                     /translation="MGTFKLALGIHNHQPIGNFQAVFDEAHRKAYLPFLEAVARSPHI
                     RLSLHQSGILWQWQKRAHPEFFRLVGDLVDRGQVELLTGGFYEPILAAIPERDIRGQI
                     ELLTDYLEEHFETRPAGFWLAERVWEPHLPRPLARAGVRFLAIDDTHFLRAGLDPADL
                     HGPYVTEHEGYALVLLPIRKRLRYLVPFGKPEEIVDCLKRAADRNPSGVAVYADDGEK
                     FGAWPQTFEHCYANGWIQNFFEALEKNRDWLEVVPLGAAARLAPVGRVYLPSGSYAEM
                     DRWALPVAAGLALEQFDKRLAAAGEFDRVEPFVAGGHWRGFLTKYEEANLLHKRMLAV
                     SARLARLEKERPDLIPALAAARDRLYAGQCNCPYWHGVFGGLYLPHIRQAVHAALIAA
                     DRMAAEREGASGVRMERRDYDADGCDDVVVETGAFTAVFRPRRGATLVQLALRRHDFD
                     VTDTLTRRREAYHLRVDEAPLGGAGHRTRSIHDRILAREPGLNALLTEDWYLKRCFID
                     HFFTADVDFDRFRSNRFGEEGDFIIEPFDIALDPADRSVAFARDGHLWRPDGVIPLRL
                     AKRFVFSETQEDIAVDYTLRCGAGRSAEVVFGIENNWTFQAGHAEDRYVLIDGVRLPD
                     SYLDGAAVHREVRTVLLADGCRRLQVGIVSDRPCEIWRLPIWTVSQSEQGFERVYQGT
                     TVVAVYRLRLTEQPTVLSLRLAAGGLAEASAHRPRLD"
     gene            10191..10931
                     /locus_tag="PKM94_04055"
     CDS             10191..10931
                     /locus_tag="PKM94_04055"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOD65844.1"
                     /translation="MVIDEIVRIMRGSRRCVVLTGAGISAESGVPTFRGKEGLWGKFR
                     PEELATMEAFLANPAIVWEWYNWRRTVLGSVQPNPGHFALADMQELFDSFVLVTQNVD
                     GLHRQAGSREVLELHGNIRRNKCLDCAEPHADSADIDPEAIPACPRCGGKIRPDVVWF
                     GEMLPEAVIERAFAESARADVFFSVGTSAIVQPAALLPVEAKHGGAALIEVNPERTPL
                     SHLADLHVSAASGEFLPRLVEAFRTARA"
     gene            11002..13143
                     /locus_tag="PKM94_04060"
     CDS             11002..13143
                     /locus_tag="PKM94_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD65845.1"
                     /translation="MIHTMDHFRTMIARVVVPVLLLLAAGCGAYYNTFYNAKRAFNQA
                     ESERRNTKPGQPERILTGDYQTAIEKSLKVVENHPNSKYYDDAVFVLGVSYYYTNQFA
                     KAERRFREILANYPDGQYARDSKLYLAKAMLRQDAVAEAMDLFAELFESDLDRSFKAE
                     AAMALGKYQFEQGRYQLANRYFLAIRDSLGADIDKRVAQRYIADGLMAQFRYNDALAA
                     YLQVLGMDPAPPERYHAMFSAAQAAFRLQRIDVGQDYLSTLAKDNLYFDSLSSLRLAM
                     ADGYEWQGNLIKAEEMYRLVSDEAANRDHKAQANLALGLIYQYDYDDLAKAKEFYDAA
                     AAASRGSSWGQEALQLSSNIGKLETFARTVKLDSTTTQAQIDDAAYTQYQLAELYWYQ
                     LSKPDSAMAEMQYLVDSFPTAYDAPKAMIALAQMAWQSGPDSARGDSIIREMLGRYPH
                     SDFVPEALGLLGLAGTEADTGYAEWYIRRAENFAVDDENIDSALANYQVVTERFPESK
                     FYLQAKFAGIWLTETYRSPGDSSILWAYQAIADSFPSTEWGQAARSKLTGQPIRTLPK
                     AQPADTITGSRFNITDSSAIDTMAEGESSDTVSMDDKYLAIITAPDGSRCVDIPPNVG
                     IIETRKEFVYPSEAYVSRWEGQLHFQIRLDFSGEVQQAIPKTFADVQEINIRAKETVE
                     STVFDMRLIRPELLDRWFLYVFTVNLPSHLR"
     gene            13140..13934
                     /locus_tag="PKM94_04065"
     CDS             13140..13934
                     /locus_tag="PKM94_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOD65846.1"
                     /translation="MMQATCVDTHVLYKHDLSHGYFALTLAGYPRAGECRPGQFLHVR
                     LPGSEVFFRRPMSVAGVNGDRGEIDIIFKVLGRGTRLMSALHDGDPVNLLGPLGKPFR
                     PPDDGSTVVMVAGGVGFPPLLFLAEWLVQGGWDPARIEFLYGGRSTGDILCRDRIRAL
                     GVRFHPTTEDGSLGRRGLVTEPLEDLIRERGAAGLFVYGCGPEPMLKAVNDLGIARRL
                     RGELSLEAPMPCGIGVCLGCVVPRTNGEHARVCLEGPVFDIGEIAL"
     gene            13931..14848
                     /locus_tag="PKM94_04070"
     CDS             13931..14848
                     /locus_tag="PKM94_04070"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOD65847.1"
                     /translation="MKPDLRVTIAGVEFANPVMTASGCCGYGEELARLYPLCRLGALV
                     TKSITRAPRLGHKPPRTAETASGMLNAIGLANVGIDRFIAEKIPFLQQQDTRIIVNVA
                     GSTLEEYVEVCARLADVPRVDMIELNISCPNVSEGGIEIGSNPRLTEQTVTAVKRVFP
                     RPLVVKLSPNVTSIVPLAQAAAQGGADALSLINSLIGTAVDIETWRPRLTNNRGGLTG
                     PAIKPVALAMVDAVYNACPLPIIAIGGIATAADAVEFILCGAVAVQVGTALFVQPDTP
                     LRIVDDLSSYLERKSLSSIDELRGKVRKY"
     gene            14848..15768
                     /gene="pyrF"
                     /locus_tag="PKM94_04075"
     CDS             14848..15768
                     /gene="pyrF"
                     /locus_tag="PKM94_04075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOD65848.1"
                     /translation="MTALDKLLKAQSKNRSLVCLGLDLDPKKMPPEYSKSAKTMFDFT
                     MRIFEVTADCVCAYKPNIAFYESLGGEGVSLLMQIMHRIPEGIPVILDAKRGDIGNTA
                     THYAQALYDRYNADWVTLNPYMGYDSLRPFIERKDKGAFILCLTSNTGARDFQMLEID
                     GKPLYRIVAEKVEYWNKSRNCGLVVGATLPEQLAEIRRAAGDMPILIPGVGAQGGSVE
                     LAAVNGTDNFQKPAVINVSRSVLYASSGSDFAQKAREEVLKLNQAVNALRTGKKSEPL
                     QPPPLPPRDSAPPPPEPPAAPPASPEPTQP"
     gene            complement(15798..16352)
                     /locus_tag="PKM94_04080"
     CDS             complement(15798..16352)
                     /locus_tag="PKM94_04080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOD65849.1"
                     /translation="MAEKKIIPADPNFIGRHTYLRPETSVDIENIWHWRQLSGLSSLS
                     SRPMLFLTPAEAAERMKAKPRDPWDQRFAIVRKEDGQLVGAINMFDYNPLNRSIELGI
                     IIDPDEREKGYASDAMGVLCRYLFKFRGLNKVHAQTAEFNKGAIRLLGKLGFKRDGVL
                     RDHYYHAGEFHAGYIYSLLAFELD"
     gene            complement(16482..17012)
                     /gene="tpx"
                     /locus_tag="PKM94_04085"
     CDS             complement(16482..17012)
                     /gene="tpx"
                     /locus_tag="PKM94_04085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOD65850.1"
                     /translation="MTTHTAQRTVQTGGGPLPLAGRELKPGDPAPMNATLIRTDMTPA
                     PLTEHRGVPRLYSVTPSLDTSVCNKQTVMFNRKLAEIGGEFQAFAISVDLPAAQKRFV
                     EEYKVDRLTPLSDYRHVAFGEQFGVLIPDIRLLARSVFVVDRHDRVTYVQVTEAVGTE
                     PDYDRAIAALKEAMKR"
     gene            17240..18292
                     /locus_tag="PKM94_04090"
     CDS             17240..18292
                     /locus_tag="PKM94_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65851.1"
                     /translation="MKVGFRALLVAVALAGGVSLGLFELTADALTSAPPADYPAYFWD
                     RHVPRTLYVYHTGSGTIDTMSLAQAPRWGVTVSADGGRLYLAADSLVAVLSARTLQTI
                     AELPYRPGGPVAVSPDGRLVAVVGDSVVVLAAADHRPIHVDTGAVRVAGFTHDSRALY
                     CVDSPESRSIRIIRFSPEGAMAERIKFSDGFPTQVWSDPDGERLFVRIRMQDLLDRVA
                     VVDLLSGAELYSEFIFPGDGIVAGAPGWPWLVYTNSGREGGPEGSSSFKIFDLAANRP
                     VREVRTRRFVNDSTPAAFPVGQAVVTPDGRKMVALDAPEGRQLLVYDLQSDRFLDWLP
                     LGEEAAVAALSVQLRP"
     gene            18403..19110
                     /gene="prxU"
                     /locus_tag="PKM94_04095"
     CDS             18403..19110
                     /gene="prxU"
                     /locus_tag="PKM94_04095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_205240138.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:18649..18651,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOD65852.1"
                     /translation="MAEPLGCARPTGRAVGQDAVAPAAPEQQPRQEVSTMAVQVGRKA
                     PDFEAPAYHKGKFTTVKLSDSLGHWVMLCFYPGDFTFVUPTELATVAATYPELQGLDV
                     HVFSVSVDSQFVHKVWDEQELSKMVDGGIPFPMISDQAGQVGRLYGVYDENLGVNVRG
                     RFLIDPDGVVQAFEIMTPPVGRNIKETLRQVRAFQHVRKTKGAEVTPAEWEPGRAVLK
                     VGPSLVGNVWKEWKRSG"
     gene            19285..20406
                     /locus_tag="PKM94_04100"
     CDS             19285..20406
                     /locus_tag="PKM94_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOD65853.1"
                     /translation="MNPLPLEPPVRTLLGPGPSDPYPEVLAALARPTVGHLDPWFIDL
                     MDRTRELLRYAFRTDNELTIALSGPGSAGMETCLANLIEPGDTAVVCVNGYFGSRMRD
                     IVDRIGGRPVAVNDEWGRPVDPAKVEDALKKNPGAGILAFVHAETSTGAQSDAKTLAE
                     LARRHGYLALVDAVTSLAGTPLYVDDWQIDAVYSGSQKCLSCVPGLAPLSLSERALDR
                     VRRRRTPVAGWFSDLTLLAQYWSGGAKRTYHHTAPVNSIYALYAALERLRSEGLENSW
                     HRHRDNHRLLKTGLESLGLEFLVDESARLPQLNAVRVPTGVDEAAVRRRLLEEFNIEI
                     GAGMGELAGKIWRIGLMGCSSDPLKIERLLAALRKILVA"
     gene            complement(20429..22054)
                     /locus_tag="PKM94_04105"
     CDS             complement(20429..22054)
                     /locus_tag="PKM94_04105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOD65854.1"
                     /translation="MARRRKIPTKGELLQLQKLYKTDEKIGERLGGVPAYLVAYWRRK
                     KNVAKHSVPKFSEAEVRNLWERFGDDDRCGLELGISKAAFYNWRRRYGIREKPPFLKL
                     EQLELNLPGLKQRGHAVSLLGKQSAAQKIIAMAAEREKVEVGELVVVQPDVTIAHQDA
                     AAVVRAFKEIGTEYVWNPARISVALARCNDSDGCVSAADHRLVREFVRRQGIPKFYDV
                     YEGGCHQLVVEDGLVAPGQLVLGSDVYATSYGALSAFAAAISPAEMATVWATGRIWLR
                     VPPTIRIDVTGRRARGVYTRDVILYLLKRLGPQRADYAAIEYQGPAVSHMSISERVTL
                     CNASADLGAKAAICPFEAVTRRYLNARGGGRYQAMLSDKDAEYVEMFQINIDQLTPQI
                     AGPNSAAEIHAARELDGLSVQMVVIGGGTNGRFDDLRVAADILKGKQVHQDCRVLVVP
                     ASRSVYLEALKKGLIRVFAEAGAAVTDPSAGLNAARLPEQVAPGERCLASTNTSLFPR
                     AAELGAELYLCSPATAAASALNARITDPTPYVR"
     gene            complement(22054..22554)
                     /locus_tag="PKM94_04110"
     CDS             complement(22054..22554)
                     /locus_tag="PKM94_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOD65855.1"
                     /translation="MEDNAIWAPWRAGFVLGKKEDGCVFCNRIAHDRDSVENLIVFRA
                     RLNVVILNKYPYNPGHALIVPIRHLGGLDELTVDEANEFFQLTRDAVRLMERAYQPQS
                     FNLGMNLGRESGAGIPGHVHMHIVPRWVGDTNFMPVIGRTKVQSVPLDAVYERLRNEF
                     VEEFGS"
     gene            22745..23704
                     /locus_tag="PKM94_04115"
     CDS             22745..23704
                     /locus_tag="PKM94_04115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD65856.1"
                     /translation="MHYRLLARLDSAVSPVRPALLPYCRLRLGEVWCDPEGRHTVACG
                     DAADRAFVRRLFGARRATLAVQDPPYNLVMGQKLPPEAYANWCRAWVDNTDAILADAA
                     ALYIWLGADQDRHFQPLPQVMAMMAATGFASRSLITMRNQRGYGTQRNWMAVRQELLY
                     YVKGEPPFSVQYTGIPKTLRGYYKTVGGKRTENTERGRGETIRPGNVWVDIQQVFYRR
                     EENVNGCYAQKPLAAIERIVLASSRPGELVADFFSHAGTTLVACERLGRSCVTFDIDP
                     IFAEITIRRLERYRAEGRTGWQAGHPFERELQLKRHAASACAR"
     gene            23716..24204
                     /locus_tag="PKM94_04120"
     CDS             23716..24204
                     /locus_tag="PKM94_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOD65857.1"
                     /translation="MREITGTVVVAGDNITTDHICPAPCRGGKRADIVKSIFRECPAL
                     ADKKLPAEAILIAGDSFGGGPEPALAASALLAAGVRCVIARSFPREFFRPAVNAGLAL
                     VTADLRGQLSEGDRISVNLRKGTVLRGEQEFPFGKYAERVARIVEQGGLVSAVRKELG
                     KE"
     gene            complement(24269..24988)
                     /locus_tag="PKM94_04125"
     CDS             complement(24269..24988)
                     /locus_tag="PKM94_04125"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOD65858.1"
                     /translation="MALYVFSDAHLGSGAAEHENRKVAQIARLFELVRADGDRLVILG
                     DLFDFWFEYRHAIPKDHHQVLFLLTDLVRRGIAVDYVSGNHDFWMDDFFATHIGVTVH
                     RDHLDMAYGGRRLHLIHGDGLAPADRGYRLLKRILRHRFNIWLYRKLPPDWAIPLAKK
                     VSGSSREYTSRRDHTFAKDYQAYAAQRIKAGFDVVVIGHLHIPVCTALGGGIYVNTGD
                     FIHHFTYAKIADGAVTLERLA"
     gene            complement(25068..25562)
                     /locus_tag="PKM94_04130"
     CDS             complement(25068..25562)
                     /locus_tag="PKM94_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65859.1"
                     /translation="MTAYPAKLTSAIVLAAALTTLRAADCDTTGFETAVQTQQSGITE
                     ARDFVIATDSAWAAFWNQVHSLQIPVPERPAVDFSKRVVLVTAMGRRPNGCYNTEITQ
                     IEDKSDGRRVICVTDFSPAPEVMCLEAIVTPAHAVIAPPGAGEALFVHKAPPRPGADS
                     AAGR"
     gene            complement(25610..27865)
                     /gene="purL"
                     /locus_tag="PKM94_04135"
     CDS             complement(25610..27865)
                     /gene="purL"
                     /locus_tag="PKM94_04135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOD65860.1"
                     /translation="MAYQQPEVTPEMVAAHGLTKEEHERIREILGREMTWTELGIFSV
                     MWSEHCSYKNSIALLKTLPREGEALLAKAGEENAGAVDIGDGLAVVFKIESHNHPSAV
                     EPYQGAATGVGGILRDIFTMGARPIAALDSLRFGLPDNPRVRYLVDGVVRGIGDYGNS
                     FGVPTVAGEVCFDEAYTGNPLVNAMAVGLVRADRMASAVARGAGNALMIVGSKTGRDG
                     IHGATFASEELSEKSQEKRPSVQIGDPFTEKLLVEATLEIIEQDLIVGIQDMGAAGIT
                     CSSSEMSAKGKAGVELHIERVPVREPHMTPYEILLSESQERMLVCVKKGKEDAVRAVF
                     DKWGLDSTIIGHVTDDGLMTVRLDGQVVAQVPSRELVLGGGAPVYHRETRRPAYMDEI
                     VRLDPRRYPLERDWNGTLAAMLAAPNICHKGWVFEQYDSMVRTNTAVGPGSDAAVLRI
                     RKTHKALAMTTDCNGRYCYINPRLGAQSAVAEAARNIVCSGGQPLAITNCLNFGNPYK
                     PEIYYGFAEAVAGMGEACRALGTPVTGGNVSFYNEDPRRAVFPTPTIGMIGLIEHTDH
                     ITTQWFKDEGDVVCLIGRNSDHLGASEYLHTIFGETRGAVAPLDLQFERRMQEVLLAA
                     IRAGEIKSAHDAADGGLAVALAECCIARRERPIGAAITTGEDIRPDALLFGEAQSRVI
                     VSCEPGRLANLGRRFERAQIPLRKIGTVGGDRLTIDTLVTMPLADMNDAWYNAMKRFM
                     GEGQVTAPVRG"
     gene            complement(27949..28629)
                     /gene="nth"
                     /locus_tag="PKM94_04140"
     CDS             complement(27949..28629)
                     /gene="nth"
                     /locus_tag="PKM94_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOD65861.1"
                     /translation="MPREGAAARRERAAEIVRILRRHYPGACTTLAYGSVHQLLVATI
                     LSQQNTDKGVNAVTPRLFRKYPTIEAFANADVDDLRADIASINYGPTKARAIIESALQ
                     ILEQFGGEVPRTIGELVRLRGVGRKTASVVLGAGYGIAEGVVVDTHVIRVSGRLGLTA
                     HSDAARIERDLMKILPQRDWIDWTHMVILHGRAVCTARRPACPACPLAARCPAAFKFP
                     HLERGGGR"
     gene            complement(28629..29489)
                     /gene="prmC"
                     /locus_tag="PKM94_04145"
     CDS             complement(28629..29489)
                     /gene="prmC"
                     /locus_tag="PKM94_04145"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOD65862.1"
                     /translation="MTNRLRPLLARYAAQLAAAGIEQAEAELEWIFEHVLGVDRLRLY
                     LEGDDLLTDAAAAQADAIVQRRLTRHPLQFILGEAWFYGRKFSVSEAVMAPTPETELL
                     VETAVRSVGSRGLAAPRVLDVGVGSGVIAVTLALELPACRVTAVDISAEALAVARQNA
                     AAHGVRDRVDLRVSDFFSAVAADERFDLIVSNPPYIAEPDYAGLAPEVRADPKIAMTA
                     GPEGLDAIRVLVQEAPAHLVPGGRLMFEIGHDQADKVAALTARDPRYRTFVLLRDLND
                     RDRVIILGGD"
     gene            complement(29486..30484)
                     /locus_tag="PKM94_04150"
     CDS             complement(29486..30484)
                     /locus_tag="PKM94_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65863.1"
                     /translation="MKITRFALALVLTAALLYIARTNSRGRPEPYVHTDRGFTLEMMT
                     VPKAYEVDTARITATIRGPFDGSVQPVLRHARFGQDETTPLYRYRTTPLEVLDSAGGR
                     YGYLMTAGLKGGRFLYYIEVRDKIGGQRAVFLDGGRPFVMKYVGHVPTWLLLTHIALM
                     FATVYFVVIGALHGARLTGGGTDVFPMARAYGIAALCAFLGGYPFGIPMNWFTFGTTW
                     EGVPFGTDATDNKTQLLFVYLLLVVFAAFGSLTRRRVGRDAYPPRVLGWLGVLGFAVM
                     LGIYLIPHSIQFSPALTRIVCWSFIGLAAAAYFGGLAAGIVRRRAPRAHKAERRAG"
     gene            complement(30481..31566)
                     /gene="prfA"
                     /locus_tag="PKM94_04155"
     CDS             complement(30481..31566)
                     /gene="prfA"
                     /locus_tag="PKM94_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD65864.1"
                     /translation="MLDLVAKLENRLAAIDREMAEPGAASDQRRMIELGRERRRVEDV
                     LAIGRDYRRLRAGIEEAQAVIRADEDDELMALAREELAAYESQIHEVERALKLKLLPK
                     NPNDDKAAIIEIRAGTGGDEAGLFVGDIFRMYQRYADTRGWKLEILSSHPGLSGGFKE
                     IIFSLDGEDAYGRMKYESGVHRVQRVPQTETQGRIHTSAVSVAVLPEAEEVDIEIKEN
                     EIKVDVFRSSGPGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKVKALKVLRARL
                     LDLQIAAQEAKIAAERRSMISTGDRSAKIRTYNFPQSRVTDHRINLTLYRLDQVLAGD
                     VDLLIDELRRHEQEQQLKLQETAGAQL"
     gene            complement(31650..32621)
                     /locus_tag="PKM94_04160"
     CDS             complement(31650..32621)
                     /locus_tag="PKM94_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOD65865.1"
                     /translation="MPDLNVGGQAVIEGVMMRSADRIATAVRKPNGEILVKCEPYLSL
                     TKRHRVLGAPIIRGTVSFAEMLVVGIRTLNFSADVAMREIEAAEAEKNGAAAAPGKDK
                     KSNALVLELSVAAAFALGIGLFFFLPLALASLVGIKKDAVGFNLLAGGIRLALFVGYV
                     WMISFLKDFKRIFQYHGAEHKSIYAYEMGDDLTPGRAALYTRFHPRCGTSFILIVALT
                     AILIYAVSDSLFAVIAGHPPSLATRFAVHLALLPLVAGGSYELLKLSGRTRDHKVTRV
                     LIQPGLWLQAITTREPSFDQLEVAIVALETALGRTESKVAATRIPVS"
     gene            complement(32728..32943)
                     /gene="rpmE"
                     /locus_tag="PKM94_04165"
     CDS             complement(32728..32943)
                     /gene="rpmE"
                     /locus_tag="PKM94_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD65866.1"
                     /translation="MKAEGHPKTFDVTITCSCGATYPNRSTKKNISVEICSNCHPFFT
                     GKQRLIDTAGRVERFIQKYKKYQKDSK"
     gene            complement(33129..33791)
                     /locus_tag="PKM94_04170"
     CDS             complement(33129..33791)
                     /locus_tag="PKM94_04170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD65867.1"
                     /translation="MGDEQTRRYYRRRAPEYEQIYYRDNPPRRKEIDDEAAFLRGLAD
                     GREVLDLGCGTGYWTQILAERAALVAAADIAPEMIAQAGRKEYRRPPHFLIADLEHPP
                     FAEGAFDLVVLAFWLSHQPRETFPTFFPMLGRLVRRGGRLWMIDNNPPAEGRSLPAAG
                     VDAHGNNFRRRFLDNGEPFVILKNYFQRDELVSLCAPYGRVDRLVYGTYYWSILLSDM
                     NL"
     gene            complement(33784..34401)
                     /locus_tag="PKM94_04175"
     CDS             complement(33784..34401)
                     /locus_tag="PKM94_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD65868.1"
                     /translation="MISLLVISGSPVPGASTDILLRQAADAVRRGLRGAARVRTTFVK
                     LNDLRILPCQACGEAPTPQFCFFDDDMAPLYRELARCDCLLFGSPIHFDTVSSQAKLF
                     IDRCNCVRPADFRGADPDHGFLKLIARKRPGAMVLVSGERQWTEGARRVIAGFFKWAE
                     VTNEGLLTYRTRGLEAGEAAGSAAALAEADALGHRLAGLLRRRCG"
     gene            complement(34394..36292)
                     /locus_tag="PKM94_04180"
     CDS             complement(34394..36292)
                     /locus_tag="PKM94_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOD65869.1"
                     /translation="MTLLAAEGVSKKYQDQVLFDAVSFTITAADRIGLVGRNGIGKTT
                     LFELLTGRVAADAGTVTLAKHCVIDYVPQDKTSVLDWELFALVASARRDLAQMRCEIT
                     ELEEHLADDPHDARRLERLGQVQHEFEAAGGFGFESEVRLILEGLGFERARHRDRLAT
                     FSGGEKNRAGLAMALAGCGNLLLLDEPTNHLDIESTVWLEEYLRKLEKACVIVSHDRR
                     FLSRTVDKVWEIQFGKIHQYVGGFEKYLVERAERKRLHEHHYRHQQEEIRRIEEFIRR
                     NMAGQKTRQAQSKLKYLSRIKRIPPPRGEGAGPSIAVRSSGRSFAHVLEVRSVTLAYG
                     AEPVLRNVSFDLYRGEKIGLVGRNGSGKSTLLKALIGELAPVSGELRLGANVDVVYFD
                     QDLDNLRLEATVLDNIWEEDPMAEVGAMRSFLARFGFTGEDCFKRVSALSGGERTKLS
                     LARIIYHPANFLIFDEPTNHLDIDSREALEEALGRFDGSCLIVSHDRHFLDRVADRII
                     HLAGGTAFVYGGGYSHFEEKQAEAAAAALEAAPRPDKEKSREVYQAFKEASRVKARLK
                     KALKSTRSKIADHERELEELEDGIARRIPRTDWEALEAASRRKQEVEAALLALYGELE
                     ELERRDCD"
     gene            complement(36289..>37581)
                     /locus_tag="PKM94_04185"
     CDS             complement(36289..>37581)
                     /locus_tag="PKM94_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65870.1"
                     /translation="RTDLITSLYLDGRRVAQTDAAAPAGGDEAVRFTQSVARGGFHSG
                     WVELSDDRFPSDNRYYFTFRIPEQFTVLMVAGDPAAQFISLALTPSGDLAQYWSVKQA
                     RPEDLGTVDLAEYDVIMLPGTPAIADAYVGRIKAAVRRGTSLFLTYGGGADAASFNRT
                     WADIAGVVIDEAARTDFSRAGYYTLESVVKEHPIFSVFSFAEKLPDLKFYTLPRLHTV
                     GEAAEIATFSGGRPALVEARYGEGRVVTFAGPMAPAYSDMVGHAFFVPFVSRVAEYLA
                     ADLSSYDLKLYARASTPRVISLRGAISGSLELIAPDSSRYYLSPEESEGRVLVRAQPV
                     ERPGIYQVRYAGQEVDRFAVNIDPAEGDLAAADVDRFAQAVGAPEHRELAAEGNLAGL
                     IAESRYGRELWQLFLWIAAGLLLAEMLLARGAPPEEPG"
BASE COUNT         6002 a        13277 c        11918 g         6384 t
ORIGIN      
        1 gaactggctg cggctgttcg gctggctggc ggtcggtttt gtgatctact tcacctacag
       61 ccggcgccac gccgtgacgc gccgtctgcg ggcggccggc gccgcgccgg agcgaaaaga
      121 agccgggaca cggtgagcgc cttcgtcccc gcggcgcggg agccagccgc gcggggcgtg
      181 cgcgcccgcc gggggcggct gcgggcggtt ttcgccctca cgcctccccg gcgccgtggc
      241 acttcttgta cttcttgccc gatccgcatg ggcagggatc gttgcgcccg actttcggca
      301 tctcccgctt gatcgtgcgt gcctggcgcc cggcttgcga ggcctccgcc atcgggtccc
      361 gctcgtgcgg ggccatgccg gccgggccgg cacccgcccc ggtcggaccg gcgccgcgca
      421 ttcccgcgcc caccggttct cccggagcgc gcccgccgcc cagtcccatg ttggtggcgt
      481 cggcatggct ggtctgaaga ttctgcggga cccgccggcg gtcggtgcgc gactgctcgg
      541 ggatgttcac ccgcagcttg tacacgaggt tgacggcctc gcggtcgatc cgcaccacca
      601 tgtcctcgaa catcttgaaa gcctcgcgct tgtagatgtc gatcggtttg cccatcccct
      661 ggtggtaggc gcgcagcccg atccccgtgc gcagctcgtc catctcggcc agatggtcgc
      721 gccagtggcg gtcgatcgtc gacagcaccg cgtaccgctc cagctgccgc atgatctccg
      781 ggccgtaggc ggcctccttc tgccggtaga acgccatcac cgccccgagg agctgttcgc
      841 ggagtttcgc ctgggtgaac tcggcgatgt cgtccgttct gacctcgaaa ttgagcaggt
      901 agatctcgcg cagcttgtcg cgcaggccgg tccagttcca ctcttcgggg tgatccttct
      961 ccggggcgta ttccgccaga agatcgtcga gcaccccctc gatgagctcg accacttcct
     1021 cctgaatgga cggccgctcg agcgcggcga ggcggcgctc gtagatccac ttgcgctgga
     1081 cgttcatgac gtcgtcgtac tccagcgtgt gtttgcggat ggcgaaattc tgggcctcga
     1141 ccttcttctg ggcgcgctcg atcgcccgcg tgaccatccc gtgggtgatc acttccccct
     1201 cctgtacgcc gaggcggtcc atgatcgcgc cgaggcggtc gccgccgaac agccgcatga
     1261 ggtcgtcttc gagcgagagg aaaaacagcg aggatccggg gtcgccctgg cggccggcgc
     1321 ggccgcggag ctggcggtcg atgcggcggg cctcgtgccg ttcggtgccg ataatgtgga
     1381 ggccgcccag ttcgaccacg cccgggccga ggcggatgtc ggtgccgcgg ccggccatgt
     1441 tggtggcaat cgtcaccgcg ctccgctgcc cggcctgggc gacaatctcg gcctcccgct
     1501 ggtgctgttt ggcgttcaac acgttgtgcg ggatccccgt ccgcttcaac atccgcgaaa
     1561 gcgtctccga cacttccacc gaggtcgtgc cgaccagggc cggccggccc gcgttgtatt
     1621 gggtctggat ctcctcgatg atcgcgttgt acttctcccg acgcgtccgg aaaatctgat
     1681 cgtccatgtc tttacggatc accggcttgt tggtcgggat cacggtcaca tcgagcttgt
     1741 agatgtccca gaactcggcc gcctcggtct cggccgtccc ggtcatgccc gcgagcttct
     1801 catacatgcg gaaataattc tgaagcgtga tggtcgcaag cgtctgcgtc tccgcctcga
     1861 tcttgacgcc ctccttggcc tcgatcgcct ggtggaggcc gtcggagtac cgccgccccg
     1921 acaggatgcg ccccgtgaac tggtcgacga tcatcacttt gccgtcctgc agcacgtact
     1981 cgacgtcctt ctcgtacagc gagtacgcac ggagcagttg gttgatcgcg tgcactttct
     2041 cggaccgctc ggcgtggacc gcgtaggcgg cgtcgatccg cttctgccgc tcctctggcg
     2101 ccagcgactg atccccctcg atctccgaca gcatactcga caggtcgggc agctcgaaca
     2161 gattctgctc ttccttcgtc agttgggcgg tgccgaggtc ggtcaaggcg atcgtgtgct
     2221 cgcgttcgtc gatgaagaaa tagagccggt cgtcgatctc gtgcagtttc ttgtcgcgca
     2281 tgtaggcgga ctcgacgtcg gtgatgagct tggccacccc cggctctttc tccaccttga
     2341 gcaggcgctt gttcttcggc gcgccccgcc gggcggccag caggagagtg cccgcctgga
     2401 aatgatcccc gtcctcggcg gccaggatct cctccgcctc ggccaccatg tcgttgatca
     2461 ggcgcgtctg cttgcgcacc agcgtctcca ctgtcgccag catctcccgg tagcggtcgt
     2521 ggacagtcgt ggtctcgacc tgcccggaga tgatcagcgg cgtgcgcgcc tcgtcgatca
     2581 ggatcgagtc aacctcgtcg atgatcgcgt agccgaaatt ccggtgcacc cggtcctcca
     2641 cccgctgcgc catgttatcg cggaggtagt cgaagccgaa ttcattggcg gtgccgaagg
     2701 tgatgtcgca gaggtactgg gtgcggcgtt ccgcgttggt catctggttc tgaatgcacc
     2761 ccaccgtcag cccaaggtac gtgtaaatcc accccatcca ctcggagtct cggcgggcga
     2821 ggtagtcgtt gaccgtgacg atgtgcacgc ctttgccggc cagcgcgtta agataggtcg
     2881 gcagggtcgc caccagcgtc ttcccttccc ccgtcgccat ctccgcgatc ttcccctggt
     2941 ggagcacgat gccgccgatc aactgcacgt cgaacgggac catgttccat tcgaccggca
     3001 ggccgaccac ctccagggtc ttcccgacgt ggcggcggca ggcctccttg acgaccgcga
     3061 aggcctcggg caagaggtcg tcgagcgtcg ccccctcggc cagccgctgc cggaactccg
     3121 ccgtcttgcc cgtcagctcc tcctcggtca gcacagagag tctctcgtag tgctcgttga
     3181 tctcggccac cagcggggcg atcttcttga tgtcgcggtc gtgtttggag ccgaaaatcc
     3241 tggtcagcaa attcgccata cctgcattcg ccgcttcggg tttaccttaa ccttatacaa
     3301 taatacatgg gcccttccgg ggcaatcgtc ttgctggagc atacgccccg gcggacgcgg
     3361 gagcaagata aagcgccccc cggcccccgc acttcgtcgt tgaccggccc gccgccgggc
     3421 cgctatcctg tcggggcggc ggcgcaccgc cgacccggcc gcgcgccggc ggcgccgccc
     3481 cgaaaaaccc tgtatgactg cgccaaccga ccgcccggcc gtcccgagaa tcccccccgg
     3541 ggtatgggtc ctcggctttg tgagcatgct tatggacgtc tcctcggaga tgatccatgg
     3601 gctgctgccg ctgtttctca ccaccgtgct gggcgcgggc gtgatcgctg tcggcctgat
     3661 cgagggtctg gccgaggcca ccgcgctcat cgtcagggtc ttctccggca tagtgagcga
     3721 ctacctgggc aaacggaaag ccctcgcgct cctcggctac ggactggggg cgctgaccaa
     3781 gccgcttttc gccctggcgc cgcacgcagg cgtggtgctg gcggcgcgtc tggcggaccg
     3841 cgtcggcaaa gggatccggg gggcgccgcg cgatgcgctg gtggcggaca tcacgccccc
     3901 ggccctgcgc ggagcggcct tcggtctccg ccagtcgctc gacaccgtcg gggcctttct
     3961 cggcccgctg gcggcggtcg ggctgatgct tttgtggcac gacaacttcc gcgccgtgtt
     4021 cggcctggcc gtaatccccg gcgcggcggc cgtcgtcctg ctcgccctcg gtctccgcga
     4081 acccggccgc ccccccgcgg ggcgccgcac caaccccatt cgccgcgaca atctccgcct
     4141 cctcggcccc gcctattggt gggtagcggc tatcggggcc gtcttcaccc ttgcccgctt
     4201 ctccgaagct tttctcgtgc tccgggcgca gcaggcggga atggcgatcg cgctcatgcc
     4261 gctggtgatg gtagtgatga acgccgctta cgcgttgacc gcctacccgt tcgggaggct
     4321 ctccgaccgg ctcagccaca ccgccctgct ggccgccggc ctggtcgtgc tgatcgcggc
     4381 cgatctcgtc ctcgccgccg ggtccggatg gaggctggtc ctggtcgggg tgactctctg
     4441 ggggctgcac atggggctga cccaggggct gctcgcccgc atggtcgccg ataccgctcc
     4501 cgctactctg cgcggcaccg ccttcggctt cttcaacctc atcagcggac tggccatgct
     4561 gcccgccagc gtcgtcgccg ggctcatgtg ggcgcgtttc ggcgccgcgg cgacgtttta
     4621 caccggggcc ggcttcggcg ctctggcgct ggctggactg ctcgcccgcc gacccgcccg
     4681 ctccgcctga accgcggcgg ccgccgggcc gcgcctctca gcagaagtag gagagaatga
     4741 tgctcagcgg gtggaacagc cgccgcgggc tttccgtctc ggccatgaac gcctccacct
     4801 gccgctgcag gtagcggctt cgcgtgatgc ggcgcgccac atagtcgttc agccgccgcc
     4861 gccccaggta acgctccgcg acggcgtatc cgcggtagcg gggacggagc tcgcggaggc
     4921 gttcgctgaa ccggccgagg agggacggat catcggcggc gagcgcctcg tgcagcgtct
     4981 ccgcggccag cgaccccgtt tccatcgcct tgccgattcc ctccccggta aacgggtagg
     5041 tcgccccgat cgtttcgccg atcgccagca cgttgccgca atgggcggtt gcgagcccgc
     5101 gcagcccgca gcggagagcg gcgccggcga tgggggagac gatctccgcc ccggccatga
     5161 tctcgcgcgc gggaggaaac cgctccataa accggtgcag cgtccgcttc agcatgtgcc
     5221 gtccgtcacg cacgtggcgg tacatcgtcc cgcacccgac gttgtacagg aaggcgcccg
     5281 gatggccgtg atcttcgccc agcggcacga tccagccgta gccggggagg atggactcgt
     5341 cgtacgagat gatcgtgtca tcgcggcggc ggggcgaacg gacatagcag cgcagcgcga
     5401 tcgcgctcgg ttccggatcg gtgaccaggc cgatgcgctg ggcgaggtcg accaccgccc
     5461 cggtcgcaac cgccgcgaaa cgggcggtga ttggttcggc gacgcccgag agatgcacgc
     5521 gcaccaggcc gtccccggcc gccggctcca ccccggcggc gttgcccgcc aggacgtgcg
     5581 cccccgccgc tgccgcgtgg cggcagagct cgtagtcgag gcgcttccgg ctcagcgcga
     5641 gatactcgcc ggggacggcg aagcggatcc ccgaggggct ggcgacctcg atggtccggc
     5701 tcgcatgggc gagcgccgcg atgcgggcgt ggagatccac ccggcggagc atcgccagcg
     5761 cgtccggcac gagcaggtcg ccgcacactt tgtggcgggg aaaccgttct ttgtcgatga
     5821 ccagcacgtt gtggccggag tgcgccaact ggcgcgcgca gaccgacccg gccggaccgg
     5881 ccccgatgat gacggcatcc cacggtcgcg ccgcgcaagc acgcggatcc ccccgcagga
     5941 cctgatcgta tgaccattcc gaaccggaca tcgtgaccgc atcctttcgt tccaccacga
     6001 tacactgcgc gggcgcgcgg gtcaacccgg tcggcggtcg cgggacaaaa tcgcttgata
     6061 cgctccgcgt tcgggcatac ctttacgacg acgtatgacg gaacgggaaa agggtatcgt
     6121 ggtggccacc cgcgggcggc tctttgaggt gtgcgcggcg gacggtgcgc gcgtgctctg
     6181 tgaagtccgc cgcaaagtca agcaccagac ggagcatgag acgccggtcg cggtcggcga
     6241 tgacgtcgtg atcacgcgcg tctctcccgg cggcggggcg attgacgagg tactcccccg
     6301 ccggaccgcc tttgtccggc cggccaaagg ccgggaaacc aaaaaacagg tcctggccgc
     6361 caatctcgac cgcctcgcgg cggtcgtgtc gatccaggcc ccgccgctca ggcccggcct
     6421 gatcgaccgc ttcctcatcg ccgcccgtct cggcggactc gaaccgctgg tcgtgatcaa
     6481 caaggtcgat ctcgagccgc cccccggact cgatgacgtg gtggcggtct accggtcgct
     6541 cggctgcggg gtttttctcg tctcggggct cgccggaacc ggcctgggcg agctgtgcga
     6601 ggcgctccgc ggccaccgga ccctcttcgc gggacattcg ggcgtgggga agacgacgct
     6661 tctgaacgcc tttgtgccgg gccttcgcct gcgcgtcggc caggtctctc cgatcacccg
     6721 ccgcggccgg cacacgacga ccacgatcga aatgtgcgaa ctgcccaccg gcggatttgt
     6781 ggtcgactcg cccgggctgc gcgtcctcgg gctgtgggag atcaccccgg acgacctccg
     6841 cggcatgtac cacgaattcg aaccgttcgc cggcgagtgc cgctaccagc cctgctccca
     6901 cacccacgag ccgggctgcg cggtcaaaaa ggcggtggcg gccgagaaga tccacgccct
     6961 ccggtacgag aattacctgg ccatcgcggc ctccctcccc tgagggaccg gcgcgataaa
     7021 cgaatccccc tccggggccg atacagagag agatagcagg aaaccggtat ggcggcaaaa
     7081 aacatcgaat tccgcgtcgg cgtgattatc ctcatcggcc tggtcgtcct gggcgcctcc
     7141 ctctactggc tccagggcta caaactggag cggaacgccc aggtgatcca ggtgctcttc
     7201 gatgacgtcg gcaccctctc ggtcggcgac cgcgtcaccg tctccggcgt ccaccggggc
     7261 aaggtcgacc ggcttgatct ggtcgacgac ggtgtgctcg tgaagatcct gctctatcgc
     7321 gacgtcgtcc tccaccgcga cgcccgcttc attatccgca acatgggcgt tatgggcgag
     7381 cggttcatcg cgatcacgcc ggggaaagag ggcgcgccct tcgacttcga ttccgtggcg
     7441 gtcggcgaat acgataccgg gctgccggaa gtcatgggag agctggggga gatggtgatc
     7501 gaggtgcgca acctcgtcgg gtcgttccgc cgcaacctcg gctccgactc gaccctggag
     7561 gcgctcaatc agacggtccg caatctggag gacgtctccg gatcgatggc cgaatttctc
     7621 gacgccaacc agagcggcct caattcggct atccgcaact tccagagcgc cgcgggccgc
     7681 ctcaatgagc tcgtcacccg caacgccgat caggtggact cgaccctggc caccgtcaac
     7741 cgcacctccg cgcgcctcga ggagttcacc taccggctcg acaccctggc cggcgcggcc
     7801 cgccagttcg ccgacatgct caacaatccc gacggcaccg cgcagctgct cctgcaggac
     7861 cgccgcctgt acgacgatct gcgctcgacg gccgacaaca tcgatgatct ggtcaaagac
     7921 atccgggcga atccgcgcaa gtatctcgat ctgaaagtgg agatattcta gacgggatgg
     7981 gcacctttaa gctggccctc ggcatccaca atcaccagcc gatcggcaac ttccaggccg
     8041 tcttcgacga ggcgcaccgc aaggcctacc tgccctttct cgaagccgtc gcgcgcagcc
     8101 cccacatccg gctctcgctc caccagtcgg gcatcctctg gcagtggcag aagcgggccc
     8161 accccgagtt cttccgcctc gtcggcgacc tcgtcgaccg cgggcaggtg gaactgctga
     8221 ccggcggctt ctacgaaccc atcctcgccg ccatccccga acgggacatc cgcggacaga
     8281 tcgaactttt gaccgactac ctcgaggagc atttcgagac ccggccggcc ggcttctggc
     8341 tggccgagcg ggtctgggaa ccgcacctgc cgcgcccgct cgcccgggcg ggcgtgcggt
     8401 ttctggccat cgacgatacc cacttccttc gcgccggtct cgacccggcc gatcttcacg
     8461 gaccctacgt gaccgagcac gaggggtacg cgctcgtgct cctgccgatc cgcaagcgcc
     8521 tccgctatct cgtgccgttc ggcaagcccg aggagatcgt cgactgcctt aaacgggcgg
     8581 ccgaccgcaa cccgagcggg gtcgccgtgt atgccgatga cggcgagaag ttcggcgcct
     8641 ggcctcagac gtttgagcac tgctacgcca acggttggat ccagaacttt ttcgaggccc
     8701 tggagaagaa ccgcgactgg ctcgaggtgg tgccgctcgg ggcggcggcg cggctggcgc
     8761 cggtcggccg cgtttacctg cccagcggat cgtacgccga aatggaccgc tgggcgctgc
     8821 cggtggctgc cgggctggct ctcgagcagt tcgacaagcg cctcgccgcg gccggcgaat
     8881 tcgaccgcgt cgagccgttc gtcgctggcg gccactggcg cggctttctc accaagtacg
     8941 aagaggccaa cctcctccac aagcgcatgc tcgccgtttc cgcccgcctc gcccgtctcg
     9001 aaaaggagcg ccccgacctg atccccgcgc tcgcggcggc gcgcgaccgc ctctacgccg
     9061 ggcagtgcaa ctgcccctac tggcacggtg tgttcggcgg gctctacctg ccgcacatcc
     9121 gccaggcggt ccacgccgct ctcatcgccg ccgaccgcat ggcggccgaa cgggagggag
     9181 cctcgggcgt gcgcatggaa cggcgggact atgacgccga cggctgcgat gacgtggttg
     9241 tcgaaaccgg cgccttcacc gccgtcttcc gcccgcggcg cggcgcgacg ctcgtccagc
     9301 tcgccctgcg gcgccacgat ttcgacgtca ccgacacctt gacccgccgc cgcgaggcct
     9361 accacctccg ggttgacgag gcccccctcg gcggcgccgg gcatcgcacc cgctccatcc
     9421 acgaccggat cctcgcccgg gaaccgggcc tcaacgccct cctcaccgaa gactggtacc
     9481 tcaaacggtg cttcatcgac cacttcttca ccgccgacgt cgatttcgac cgcttccgct
     9541 ccaaccggtt cggcgaggag ggggacttca tcatcgaacc gttcgacatc gccctcgatc
     9601 ccgcggaccg gagcgtggcg ttcgcccgcg acgggcacct gtggcgacct gacggcgtca
     9661 tcccgttgcg gctggccaaa cggttcgtct tctcggagac gcaggaagat atcgccgtcg
     9721 actacaccct ccgctgcggg gccgggcggt ccgccgaggt ggtcttcgga atcgaaaaca
     9781 actggacctt ccaggccggg cacgccgagg accgctatgt cctcatcgat ggcgtgcggc
     9841 tgccggacag ctatctcgac ggcgccgccg tccatcggga ggtgcgcacg gtcctgctcg
     9901 ccgacggctg ccgccgcctc caggtgggga tcgtctccga ccggccctgc gagatctggc
     9961 gtctgccgat ctggaccgtt tcccagtcgg agcagggatt cgagcgcgtg taccagggga
    10021 ccacggtcgt ggcggtgtac cggctccgcc tgactgagca gccgaccgtc ttgtcgctgc
    10081 gcctggcggc gggggggctc gcggaggctt cggcgcaccg cccccggctg gactgacccc
    10141 gcccggcccc ttattccgtt tgattttccg cccccgaacc gtatcttccc gtggtgattg
    10201 acgaaatcgt caggatcatg cgcgggagcc gacggtgtgt cgtgctcacc ggagcgggca
    10261 tctcggccga gtcgggggtg ccgacctttc gcggcaaaga ggggctgtgg gggaaatttc
    10321 gccccgaaga gctcgcgacc atggaggcct tcctggccaa ccccgccatc gtgtgggaat
    10381 ggtacaactg gcgccggacc gtcctgggca gcgtccagcc gaacccgggc cacttcgccc
    10441 tcgccgacat gcaggagctg ttcgattctt tcgtgctcgt gacccagaat gtcgacggcc
    10501 tccaccgtca ggcaggctcc cgcgaggtgc ttgaactcca cggcaatatc cggcgaaaca
    10561 agtgcctgga ctgcgcggaa ccccacgccg acagcgccga catcgacccg gaagccatcc
    10621 cggcctgccc gcggtgcggg ggtaaaatcc gccccgacgt cgtctggttc ggagagatgc
    10681 ttcccgaagc cgtgatcgag cgggcgtttg cagaatcggc ccgcgccgac gtcttcttct
    10741 cggtggggac ctcggcgatc gtccaaccgg ccgctctgct gccggtggag gccaaacacg
    10801 gcggcgcggc tctcatcgag gtcaacccgg agcggactcc cctgtcgcac ctggccgacc
    10861 tgcacgtgtc ggccgcctcg ggagagttcc tcccccgcct ggtggaggct tttcggaccg
    10921 cccgcgcctg agcggcggcg ctgaaccggt tattccgccc ggctgtataa ggggcgcggt
    10981 gcaaggcgcg ccgaaacaag cgtgattcac acaatggacc acttccgcac catgatagcc
    11041 cgagtcgtcg tcccggtgct cctgctgctc gccgccgggt gtggcgctta ctacaacacg
    11101 ttctacaacg ccaagcgcgc cttcaaccag gccgagagcg agcgccgcaa caccaagccg
    11161 ggtcagcccg agcggatcct caccggcgac taccagaccg cgatcgaaaa gtccctgaaa
    11221 gtggtcgaga atcaccctaa ctcgaagtac tacgacgatg ccgtgttcgt gctcggcgtg
    11281 tcctactact acacgaacca gttcgccaag gcggagcgcc ggttccgcga aatcctcgcc
    11341 aactaccccg acggccagta cgcccgcgac tccaagctgt acctggccaa ggccatgctc
    11401 aggcaggacg cggtagccga agccatggac ctgttcgccg agctcttcga gagcgacctc
    11461 gaccgctcgt tcaaggcgga agcagctatg gcgctcggca agtaccagtt cgagcaggga
    11521 cggtaccagc tcgccaaccg gtacttcctg gcgatccgcg actccctcgg cgccgatatc
    11581 gacaagcgcg tggcccagcg gtacatcgcc gacggcctca tggcgcagtt ccgctataac
    11641 gacgccctcg cggcctacct ccaggtgctc ggtatggacc cggccccgcc tgagcgctac
    11701 cacgccatgt tcagcgccgc ccaggccgct ttccgcctgc agcggatcga cgtcggccag
    11761 gactacctaa gcacgctggc taaagacaat ctctactttg actccctgtc gtcgctccgg
    11821 ctggccatgg ccgacggtta cgagtggcag gggaacctca tcaaagccga ggagatgtac
    11881 cggctggtgt ccgatgaggc ggccaaccgg gaccacaagg cccaggccaa cctggcgctc
    11941 ggtctgatct accagtacga ttacgacgat ctggcgaaag ccaaggagtt ttatgacgcg
    12001 gcggcggccg ccagccgggg ttcctcgtgg ggacaggagg ccctgcagct ctcctcgaac
    12061 atcggcaaac tggagacctt cgcccgcacc gtcaagctcg attcgaccac cacccaggcg
    12121 cagatcgacg acgccgccta cacccagtac cagctcgccg agttgtactg gtaccagttg
    12181 tccaagccgg attcggccat ggccgaaatg cagtacctgg tggactcatt cccgaccgcc
    12241 tacgatgcgc cgaaagcgat gatcgccctg gcccaaatgg cctggcagag cgggccggac
    12301 tcggcccggg gggattcgat catccgcgag atgctcgggc gctaccccca ttcggatttc
    12361 gtaccggagg cgcttggcct gctcggactg gccggcaccg aggccgacac cggttatgcc
    12421 gagtggtaca ttcggcgagc cgagaatttc gccgtcgacg acgaaaacat cgactccgcc
    12481 ctagccaact atcaggtcgt gaccgaacgc ttccccgagt ccaaattcta cctccaggcc
    12541 aagttcgcgg ggatctggct caccgagacg taccggagtc cgggggactc gtcgatcctc
    12601 tgggcctacc aggccatcgc cgactcgttt ccgagcaccg aatggggcca ggccgcccga
    12661 agcaaactca ccggccagcc tatccgaacc ctcccgaaag cccagccggc cgacacgatc
    12721 accggcagcc gcttcaacat caccgacagc tccgccatcg acacgatggc cgagggggaa
    12781 tcgtcggata cggtcagcat ggacgacaag tacctggcca ttatcacggc ccctgacggc
    12841 tcccgctgcg tcgacatccc ccccaacgtc ggcatcatcg agacccgcaa ggagttcgtc
    12901 taccccagcg aggcctatgt gtcgcggtgg gaaggacagc tccactttca aatccgactt
    12961 gatttttccg gagaagttca gcaagctatc ccgaagacat tcgcggatgt tcaggagatc
    13021 aacattcgcg ccaaggaaac cgttgagtcg accgtgttcg acatgcgcct catccggccg
    13081 gagctgctcg accgctggtt tctgtatgtg tttacggtca atttgccgag tcacctgaga
    13141 tgatgcaagc cacgtgtgtc gacacgcacg ttctctacaa acacgacctc tcccacggct
    13201 acttcgcgct gactctggcg ggctatcccc gcgccggcga gtgccgcccc ggccagttcc
    13261 tccatgttcg gctccccggc agcgaagtct tctttcgccg ccccatgtcg gtcgcgggcg
    13321 tcaacggcga ccggggcgag atcgacatca tcttcaaagt cctcggccgc ggcacgcgcc
    13381 tgatgagcgc gctgcacgac ggcgacccgg tcaacctcct cggtccgctc ggcaaaccgt
    13441 tccgcccgcc cgatgacggc agcaccgttg tgatggtggc cggcggagtc ggctttcccc
    13501 ccctgctgtt cctcgccgag tggctggtgc agggcgggtg ggacccggcg cgaatcgaat
    13561 ttctctacgg cggccgctcg accggtgaca tcctctgccg cgaccggatc cgcgcccttg
    13621 gcgtccgttt ccacccgacc accgaggacg gttccctcgg ccgccggggt ctggtgacgg
    13681 agccgctgga ggatctgatc cgcgagcgag gcgccgccgg gctgttcgtg tacggctgcg
    13741 gaccggagcc gatgctcaag gcggtcaacg acctcgggat cgcccgccgc ctccgcggcg
    13801 agctgtcgct cgaggccccc atgccctgcg gaatcggcgt ctgcctcggc tgcgtcgtcc
    13861 cgcgcaccaa cggcgagcac gcccgcgtgt gcctcgaggg gccggtcttc gacatcgggg
    13921 agatcgccct gtgaaacccg acctgcgcgt cacaatcgcc ggcgtcgaat tcgccaaccc
    13981 cgtcatgacc gcctccgggt gctgcggcta cggcgaggaa ctcgcgcggc tctaccccct
    14041 ctgtcgcctc ggcgccctcg tcaccaagtc gatcacgcgc gccccccggc tcggtcacaa
    14101 accgccccgc accgccgaaa cggcctccgg catgctcaac gccatcggcc tggccaacgt
    14161 cggcatcgac cgcttcatcg ccgaaaaaat cccgtttctc cagcagcagg acacccggat
    14221 catcgtcaat gtcgccgggt ccacgctcga ggagtatgtc gaggtctgcg cccggttggc
    14281 cgatgtcccc cgggtcgaca tgatcgaact gaatatcagc tgccccaacg tgagcgaagg
    14341 gggcatcgaa atcggcagca acccgcgcct gaccgaacag accgtcaccg ccgtgaagag
    14401 ggtctttccg cgcccgctcg ttgtgaagct ctcgccgaac gtgacgagca tcgtgcccct
    14461 ggcccaagcg gccgcccagg ggggagccga cgcactctcg ctgatcaatt cgctgatcgg
    14521 caccgccgtc gatatcgaga cctggcgtcc ccgcctgacc aacaaccggg gggggctgac
    14581 cggaccggcc atcaagccgg tcgcgctggc catggtcgac gcggtgtaca acgcctgccc
    14641 gctgccgatc atcgccatcg gcggcatcgc caccgcggct gacgccgtcg aattcatcct
    14701 ctgcggcgcc gtcgccgtcc aggtgggcac ggcgctcttc gtgcagccgg acacaccgct
    14761 ccggatcgtc gacgacctgt cgtcctacct ggagcgcaag agtctctctt ccatcgatga
    14821 actgagggga aaggtgagga aatactgatg acggcccttg acaagctgct caaagcccag
    14881 agcaagaacc gctcgctggt gtgcctcggg ctcgacttgg acccgaagaa gatgccgccg
    14941 gagtacagca agtcggccaa gactatgttc gatttcacca tgcgcatctt cgaagtgacc
    15001 gccgactgcg tgtgcgccta caaacccaac atcgccttct acgaatcgct cggcggcgag
    15061 ggcgtctcgc tactcatgca gatcatgcac cgcatccccg agggaatccc ggtgattctc
    15121 gacgccaagc gcggcgatat cggcaacacc gccacccact acgcccaggc cctctacgac
    15181 cgctataacg ccgactgggt caccctcaat ccctacatgg ggtacgattc cctccgaccc
    15241 ttcatcgagc gcaaagacaa aggcgccttc atcctctgcc tgacctccaa caccggcgcc
    15301 cgcgacttcc agatgctgga gatcgacggc aagcccctct accggattgt cgccgagaag
    15361 gtcgagtact ggaacaagag ccgcaactgt ggcctcgtcg tcggggccac tctccccgaa
    15421 cagctcgcgg aaatccgccg cgccgccggc gacatgccga tcctcatccc cggcgtcggc
    15481 gcgcaggggg gctcggtgga actggcagcc gtcaacggca ccgacaactt ccagaaaccc
    15541 gcggtcatca acgtctcccg ctccgtcctc tatgcctcca gcggcagcga tttcgcgcag
    15601 aaagcgcgcg aggaagtgct gaaactcaac caggctgtga acgccctgcg gacaggcaag
    15661 aaatccgagc cgctccagcc cccgccgctc ccgccgcgcg actccgcccc gcctccgccc
    15721 gaaccgcccg ctgcgccccc cgcatccccc gaaccgactc aaccgtagcg gcggagcagg
    15781 cgcccgccgc ctgcggacta gtccagttca aacgccagca gggagtagat gtaccccgcg
    15841 tgaaactcgc cggcgtgata gtagtggtcg cgcagcaccc cgtcccgctt gaacccgagc
    15901 ttcccgagca gccggatcgc ccccttgttg aattccgccg tctgggcatg caccttgttc
    15961 agcccgcgga atttgaacag gtagcggcag agcacgccca tcgcatccga ggcgtacccc
    16021 ttctcccgtt cgtccgggtc gatgatgatg cccagctcga tcgaccggtt gagcgggttg
    16081 tagtcgaaca tgttgatcgc cccgaccagt tggccgtcct ccttgcgcac gatcgcgaac
    16141 cgctggtccc agggatctct cggcttggcc ttcatccgct ccgccgcctc ggcgggggtg
    16201 aggaagagca tggggcgcga gctgagcgag gacagaccgc tgagctgccg ccagtgccag
    16261 atgttctcga tgtccacgga ggtttcggga cgaaggtagg tatggcgtcc gataaagttc
    16321 ggatccgccg gaatgatttt cttttcggcc atacgcgctg actccggttt gtggctaggc
    16381 cgcagtatgg cccccgcccg ccggccccgg caactaaaaa ggaaacggcg ccgaccggga
    16441 aaccgatcag cgccgccaag ggagaatcga catccgttcc gctagcgctt catcgcctct
    16501 ttcagcgcgg caatcgcgcg gtcgtagtcc ggctccgtcc cgacagcctc ggtgacctgg
    16561 acgtacgtta cccggtcatg ccggtccacc acaaacaccg agcgggccag cagccggata
    16621 tccgggatca gcacgccgaa ctgctcgccg aaggctacgt ggcggtagtc cgacaggggg
    16681 gtgaggcggt caaccttgta ttcctccacg aaccgcttct gggccgccgg cagatcgacc
    16741 gagatcgcaa acgcctggaa ttcgccgccg atctcagcca gcttccggtt gaacatcacc
    16801 gtctgcttgt tgcacacgga ggtgtcgagc gacggcgtga cggagtacaa gcgcggcacg
    16861 ccccggtgtt cggtcagcgg ggccggggtc atgtcggtgc ggatgagcgt cgcgttcatc
    16921 ggagccgggt cgcccggctt gagctcacgg ccggccaagg gcagcggccc gccgccggtc
    16981 tgcaccgtgc gctgagcggt gtgggtagtc atgaactaat ctccattctt tcactattcg
    17041 tgggaaaacc gtttctgtcc gctttgccgt atctcaacag ccgggaaggc cgagaagttc
    17101 atatgcaatc gcccatgaca ccactttttt cagtcatttc cctgcttgag tcccctatgg
    17161 aatcggtatc gcagaccatt gtgtcattat ctgacgcata ccctggctaa catcacggcg
    17221 gaatttgatg gaggtggaga tgaaagttgg gttccgagcg ctgcttgtcg cggtcgccct
    17281 cgccggcggc gttagtttag ggctattcga attaacggcc gatgcgctga catcggcgcc
    17341 gccggccgac tacccggcct acttctggga ccgtcatgtc ccccgaacac tctatgtgta
    17401 ccacacgggc agcggcacca tcgacacaat gtcgctggcg caggccccgc ggtggggggt
    17461 gactgtgtcg gccgatggcg ggcggttgta tctggcggcc gattccctcg tcgccgtcct
    17521 ctcggcgcgc actctccaga ccattgcgga gctgccgtac cgacccggcg ggccggtggc
    17581 ggtctcgccc gatggccgcc tggtggccgt ggtgggggac tccgtagtgg ttctggctgc
    17641 cgccgaccac cggccgatcc atgtcgatac cggcgctgtc cgggtggccg ggttcaccca
    17701 tgattcccgg gcgctgtact gcgtcgattc cccggagagc cggtcgatcc gcatcatccg
    17761 tttcagcccg gagggcgcga tggcggagcg gattaagttt tccgacggtt ttccgaccca
    17821 ggtctggtcc gaccccgatg gcgagcggct gtttgtgcga atcaggatgc aggacctcct
    17881 cgaccgcgtg gccgtcgtcg acctcctctc cggcgcggag ttgtactccg agttcatttt
    17941 tcccggcgac ggcattgtgg ccggggcgcc cggctggccc tggcttgtct acaccaactc
    18001 cggccgcgag ggcggcccgg aggggtcgtc gtccttcaaa atcttcgacc tcgcggccaa
    18061 ccggccggtg cgggaagttc ggacgcggcg cttcgtcaac gactccaccc cggccgcttt
    18121 ccccgtcggc caggcggtcg tcaccccgga tggccgcaaa atggtggccc ttgatgcccc
    18181 cgagggacgc cagttgctcg tctacgatct ccagtccgat cgctttctcg actggctccc
    18241 gctgggcgag gaggcggcgg tggcggcgct ttcggtgcag cttcggccct gagcgccgct
    18301 tgaggcgggc acacctgcgc ccgccggctt gaccggccgc gcgctcttcc gtatcttggc
    18361 ctcgaatgcg cgggcaacgg gccccgcgaa aaggaggttg acatggcgga acctttgggt
    18421 tgtgcgcgac ccaccggacg tgcggtcgga caggatgctg tcgcgccggc cgcccccgaa
    18481 caacagcccc gacaggaggt atccacgatg gcagtccagg ttggacggaa ggcgcctgat
    18541 ttcgaagcgc cggcctatca caaaggaaaa ttcacgaccg tgaagctgtc cgattccctc
    18601 ggacactggg tgatgctctg tttctaccca ggcgacttca ccttcgtctg accgaccgaa
    18661 ctggcgacgg tcgccgccac gtaccctgag ttgcagggtc tcgatgtcca cgtcttttcg
    18721 gtttcggtcg actcccaatt cgttcacaaa gtctgggacg agcaggagct ctcgaaaatg
    18781 gtcgacggcg ggattccgtt cccgatgatc tcggaccagg cgggccaggt cggccgtctg
    18841 tacggcgtct acgatgagaa cctgggcgtc aatgtccgcg gacggttcct cattgacccc
    18901 gacggtgtcg tgcaggcgtt tgagatcatg acccccccgg tcggccgcaa catcaaggag
    18961 acgctccgcc aggtccgggc gttccagcac gttcgcaaga ccaaaggcgc ggaggtgacg
    19021 ccggccgagt gggagccggg acgcgccgtg ctcaaagtcg gcccgagtct cgtcggcaac
    19081 gtctggaaag aatggaagcg gagcggctga ggctgcaatc cagagccgtc ccgccgcgta
    19141 ctccgagccg ggtttggata taaacccggc tcttttccgg aggtgcggcg gacccggcgg
    19201 catttcacca tcgcgcatcg gcgatcgccg cccccgtgcg agcgtcgagg acgaacgaag
    19261 acttagtccc ggagacccga cctcatgaat cccctgcccc tcgaaccgcc ggtccgcacg
    19321 ctcctgggcc ccggcccctc ggacccgtat ccggaagtgc tcgcggccct cgcccgcccg
    19381 acggtcggcc acctcgaccc ctggtttatc gacctgatgg accggacgag ggaactgctc
    19441 cgctacgctt ttcggaccga caacgaactg accattgcgc tgtccggtcc cggctcggcc
    19501 ggtatggaga cctgcctggc gaatctcatc gagccgggcg acaccgcggt cgtgtgcgtc
    19561 aacggctact tcggcagccg catgcgcgac attgtcgacc ggatcggcgg gcgcccggtc
    19621 gcggtgaacg acgaatgggg ccggccggtc gatccggcga aagtcgaaga cgctctgaag
    19681 aagaaccccg gagccggaat tctcgccttc gtccatgccg agacgtcgac cggcgcgcag
    19741 tcggatgcga aaacgctcgc cgagctggcc cgccggcacg ggtacctggc cctcgtcgat
    19801 gccgtcacgt cgcttgccgg gacgccgctc tatgtcgatg actggcagat cgacgccgtc
    19861 tattccggct cgcagaagtg tctctcctgc gtgccgggcc tcgcgccgct cagtctcagc
    19921 gaacgcgccc tcgaccgggt gcggcggcgc cggaccccgg tggccggctg gttctccgac
    19981 ctcactctcc tggcgcagta ctggtcgggc ggggcgaaac gcacctacca tcacaccgcc
    20041 ccggtcaact cgatctacgc cctctacgcc gcgctggagc ggctccgcag cgaaggtctc
    20101 gagaacagtt ggcatcgcca ccgcgacaac caccggctcc tgaaaaccgg cctggagtcg
    20161 ctggggttgg agtttctggt cgacgagagc gcccggctgc cgcagctcaa cgcggtgcgc
    20221 gtgccgaccg gtgtcgatga agccgcggtg cggcggcggc tccttgagga gttcaacatc
    20281 gagatcggcg ccggcatggg cgaattggcc ggaaaaatct ggcgcatcgg actgatgggc
    20341 tgttcctcgg accccctcaa gatcgagcgc ctgctggcgg cgctgagaaa gatcctcgtc
    20401 gcctgaagcc gggttcttcc tgccccccct accgcacgta cggcgttggg tcggtgatcc
    20461 gggcgttcaa ggccgacgcg gccgcggtcg ccggcgagca caagtacagc tccgcgccga
    20521 gctcggccgc ccgggggaac aacgaggtgt tggtgctggc caggcatcgt tcccccggcg
    20581 caacctgttc cggcagccgc gcggcgttca gccccgccga aggatccgtg accgccgccc
    20641 ccgcctcggc aaacacgcgg atcaatcctt tcttgagcgc ctccaggtac accgagcggg
    20701 aggccgggac caccagcacc cggcagtcct ggtgcacctg tttgcccttg aggatatccg
    20761 cggccacgcg gagatcgtcg aagcggccgt tcgtaccgcc cccgatcacc accatctgca
    20821 ccgagagtcc gtcgagctcc cgcgcggcgt ggatttcggc ggcgctgttc ggcccggcga
    20881 tctggggcgt gagctggtcg atgttgatct gaaacatttc gacatattcg gcgtccttgt
    20941 cggagagcat cgcctggtag cgcccgccgc cgcgcgcgtt gagatatcgt cgcgtcacgg
    21001 cctcgaacgg gcagatcgcc gcctttgcgc ccaggtcggc ggaggcgttg cagagcgtga
    21061 cccgctcgct gatgctcatg tgcgagacgg ccggtccctg gtactcgatc gccgcgtagt
    21121 cggcccgctg cggaccgagc cgcttgagca ggtagaggat gacgtcgcgg gtgtagacgc
    21181 cgcgcgcgcg gcggccggtg acgtcgatac ggatggtggg ggggacgcgc agccagatcc
    21241 gcccggtggc ccagacggtc gccatttcgg ccggcgaaat cgccgccgcg aacgccgaca
    21301 gcgccccgta actcgtggcg tagacgtcgc tgccgagtac caactggccc ggcgccacca
    21361 gaccgtcctc gaccaccagc tggtggcagc cgccctcgta aacatcgtag aatttcggga
    21421 tcccctggcg ccggacgaat tcgcggacga gccggtggtc ggcggccgac acgcagccgt
    21481 cgctgtcgtt gcaccgggcc agggcgactg aaatccgggc cgggttccac acgtattcgg
    21541 tcccgatctc cttgaaagcg cgcaccaccg ccgcggcgtc ctggtgcgcg atcgtcacat
    21601 ccggctgcac cacgaccagt tcgccgacct ccactttctc ccgctccgcc gccatggcga
    21661 tgatcttctg cgccgccgac tgcttgccga gcaaactgac cgcgtggccg cgttgcttga
    21721 ggccgggaag gttgagttcg agctgctcga gcttcagaaa cggcggcttc tcccgaatcc
    21781 cgtaccgccg ccgccagttg tagaacgccg ccttcgatat ccccagctcc agcccgcacc
    21841 ggtcgtcatc cccgaaccgc tcccacaggt tccggacctc cgcctctgaa aatttcggca
    21901 ccgaatgctt ggcgacattc ttcttccgcc gccagtaggc cacgaggtag gcgggcacgc
    21961 cgccgagacg ctcgccgatc ttctcgtcgg tcttgtacag cttctgcagc tgcagaagct
    22021 cccccttggt cgggatcttg cgccgccgcg ccatcagctt ccgaactcct cgacaaattc
    22081 gttccgaagc cgctcgtaca cggcgtcgag cggcaccgac tgcactttgg tccggccgat
    22141 caccggcatg aaattcgtgt cccccaccca ccgcgggaca atgtgcatgt gaacgtgccc
    22201 tgggattccc gcccccgatt cccgcccgag attcattccc aaattgaacg actgcggttg
    22261 gtacgcccgc tccatcaggc gcaccgcgtc gcgcgtcaac tggaaaaact cgttggcctc
    22321 gtcgaccgtc agctcgtcga gcccgcccag gtggcggatc gggacgatca gcgcatggcc
    22381 gggattgtag ggatacttgt tgaggatgac gacattgagt cgggcgcgga agacgatgag
    22441 gttctcgacc gagtcgcggt cgtgcgcgat gcggttacag aagacgcaac cgtcctcttt
    22501 cttgccgagc acgaacccgg cgcgccaggg ggcccatatc gcattgtctt ccatgatggt
    22561 gtagaataaa caaaattacc cacactgcca agccccgccc cggggaatat caacattttc
    22621 acaagctgct gtcgtttaga tggttagcgg cgtctttctc cgcgctccgc ccccgctgtt
    22681 atcgaccgcg ccggcgggcg atgttttagt tgccgctccc acccgaccct cccatcatcc
    22741 ccgcatgcat taccgtctgc tggcccgcct cgattcggca gtcagcccgg tgcgcccggc
    22801 gctgctgccg tactgccgtt tgcgcctggg agaggtctgg tgcgacccgg agggacggca
    22861 tacggtggcc tgcggcgatg cggccgaccg cgcctttgtg cgccgcctct tcggcgcgcg
    22921 ccgcgcgacg ctggcggtgc aggacccgcc gtacaacctc gtcatgggac agaaactgcc
    22981 gcccgaggcc tacgcgaact ggtgccgtgc ctgggtcgac aacaccgatg cgatcctggc
    23041 cgacgccgcc gccctctaca tctggctggg cgccgaccag gaccggcact tccagccgct
    23101 cccgcaggtg atggccatga tggcggcgac cggattcgcc tcgcgctcgc tcatcaccat
    23161 gcgcaaccag cgcggctacg gcacccagcg caactggatg gcggtgcggc aggaactgct
    23221 ctactacgtc aagggagagc cgcccttttc cgtccagtac accgggattc ccaagacgct
    23281 ccgggggtac tacaaaaccg tcggcggaaa acgcactgag aacaccgagc gggggagagg
    23341 ggagacgatc cggcccggca acgtctgggt cgacatccag caggtgttct accgccggga
    23401 ggagaacgtg aacggctgct acgcccagaa gccgctggcg gcgatcgagc gcatcgttct
    23461 cgcctcgtcg cgccccggcg agctggtcgc cgatttcttt tcccacgccg gcaccaccct
    23521 ggtggcctgc gagcgcctcg ggcgctcctg tgtgactttc gacatcgatc cgattttcgc
    23581 cgagatcacg atccgccgcc tcgaacgcta ccgggccgag ggccgcaccg ggtggcaggc
    23641 cgggcacccg ttcgagcggg aactgcaact gaaaagacac gcggcgagcg cgtgcgcgcg
    23701 gtaagcgagg ctgccatgag agaaatcacc gggaccgtcg tcgtcgcggg cgacaacatc
    23761 accaccgacc acatctgtcc ggccccgtgc cgggggggca aacgggccga catcgtcaag
    23821 agcatcttcc gggaatgccc cgcgctggcg gacaagaagc tcccggcgga agcgattctc
    23881 attgccggcg acagcttcgg cggcggaccc gaaccggcgc tcgccgcgag cgccctgctc
    23941 gccgccggcg tccgctgcgt catcgcccgc agcttccccc gcgagttctt ccgccccgcc
    24001 gtcaacgccg gtctcgccct cgtcaccgcc gacctccgcg ggcagctgag cgagggcgac
    24061 cgcatctccg tgaacctgcg caaaggaacc gtcctccgcg gcgaacagga atttccgttc
    24121 ggcaagtatg ccgaacgcgt cgcccggatc gtcgagcagg gcggcctcgt cagcgccgtc
    24181 cgcaaagaac tcggcaaaga gtagccgcgt caaggccgcc tcgccgccga agcgggggcc
    24241 gttgggcgcg gcggttgcgc ggcgcgccct acgccagccg ctcgagcgtg accgccccgt
    24301 ccgcgatctt cgcatacgtg aaatggtgga tgaaatcccc cgtgttgaca tagattcccc
    24361 ctccgagcgc cgtgcacacc gggatatgca ggtggccgat cacgaccacg tcgaacccgg
    24421 ctttgatccg ctgggccgcg tacgcctggt aatccttcgc gaacgtgtgg tcccgccgcg
    24481 aggtgtactc gcgcgaggat cccgagactt tcttggccag cgggatggcc cagtcggggg
    24541 ggagcttgcg gtacagccag atgttgaacc ggtggcgcag aatgcgcttg aggagccggt
    24601 acccgcggtc cgccggcgcg aggccatcgc cgtgaatcag gtgcagccgc cggccgccgt
    24661 aagccatgtc gaggtggtcc cggtgaacgg tcaccccgat gtgagttgcg aagaaatcgt
    24721 ccatccagaa gtcatggttc ccgctcacat agtcgaccgc gattccccgg cggaccaggt
    24781 cggtcagcag gaacaacacc tggtggtggt cctttggaat cgcgtggcgg tattcaaacc
    24841 agaaatcgaa caggtcgccg agaatcacca gccggtcgcc gtcggcccgg accagctcga
    24901 agagccgggc gatctgcgcc accttgcggt tctcatgctc ggccgcgccg gagccgaggt
    24961 gggcgtccga gaaaacatag agcgccatag gtctgtaata tagggaagag acgagggggg
    25021 gaaacaattt tgtcgggcgg ggtgtgcgcc ccgagagccg cggccgccta cctgcccgcg
    25081 gccgaatccg cccccggccg gggcggcgcc ttgtgcacaa aaagcgcctc ccccgccccc
    25141 ggcggcgcga tcacggcgtg cgccggcgtg acgatcgcct ccaggcacat cacctccggc
    25201 gcgggcgaaa agtcggtcac gcagatgact cggcggccgt cgcttttatc ctcgatctgc
    25261 gtaatctccg tattgtagca gccgttcggc cgccggccca tagccgtgac cagcaccacc
    25321 cgcttcgaga aatccaccgc cgggcgctcc gggacgggga tctgcagact gtggacctgg
    25381 ttccagaagg cggcccaggc ggaatcggtg gcgattacaa agtcccgcgc ttccgtaatg
    25441 cccgactgct gggtctgcac cgccgtctca aagcccgtcg tgtcgcagtc ggcggcgcgg
    25501 agggttgtca gcgccgccgc cagcacgatc gcgcttgtca gtttcgccgg gtatgccgtc
    25561 attgcacctt caccgtgtgt ccttgccgcc gccggggccg ccggcccgct cacccccgca
    25621 ccggcgccgt gacctgccct tctcccataa accgcttcat cgcgttgtac caggcgtcgt
    25681 tcatgtccgc gagcggcatc gtcacgagcg tgtcgatggt gagccggtcg ccgcccaccg
    25741 tgccgatttt ccgcaacggg atctgtgcgc gctcgaaacg ccgcccgaga ttggcgaggc
    25801 ggccgggttc gcacgacacg atcacccgcg actgcgcctc gccgaacagg agtgcgtcgg
    25861 ggcggatgtc ctcgccggtg gtgatcgccg cgccgatcgg gcgctcgcgg cgggcgatgc
    25921 agcactcggc cagggccacc gccagcccgc cgtccgctgc gtcgtgcgcc gacttgatct
    25981 cccccgcgcg aatggccgcc aggaggacct cctgcatccg ccgctcaaac tgcagatcga
    26041 gcggcgcgac cgccccccgg gtttcgccga agatcgtgtg gagatactcg ctcgccccga
    26101 ggtggtcgct gttgcggccg atcaggcaga cgacgtctcc ctcgtctttg aaccactggg
    26161 tcgtgatgtg gtccgtgtgc tcgatcaggc cgatcatccc gatcgtcggc gtcgggaaca
    26221 ccgcccgccg gggatcctcg ttgtagaacg agacgttccc tccggtcacg ggcgtgccga
    26281 gcgcccggca ggcctcgccc atcccggcca ccgcctcggc gaacccgtag tagatttccg
    26341 gcttgtaggg gttgccgaaa ttgaggcagt tggtgatcgc cagcggctgg ccgccggagc
    26401 agacgatgtt gcgggccgct tcggccaccg cgctctgcgc gccgaggcgc gggttgatat
    26461 agcagtagcg tccgttgcag tcggtcgtca tggcgagcgc cttgtgcgtc ttgcggatcc
    26521 gcagcacggc ggcatccgag cccggcccga ccgccgtgtt cgtgcgcacc atcgaatcgt
    26581 actgctcgaa cacccacccc ttgtggcaga tattcggcgc cgccagcatc gccgccagcg
    26641 tcccgttcca gtcgcgctcc aaagggtacc ggcgcggatc gagccgcacg atctcgtcca
    26701 tgtaggccgg gcgccgcgtt tcccgatggt acaccggcgc gccgccgccg agcaccagtt
    26761 cccgggacgg tacctgggcg accacctgcc cgtccagccg gaccgtcatc agcccgtcgt
    26821 cggtgacgtg cccgatgatc gtcgaatcca gcccccactt gtcgaagacc gcgcgcaccg
    26881 cgtcctcttt ccccttcttc acgcacacga gcatgcgctc ctgcgattcg gacaggagaa
    26941 tctcgtacgg cgtcatgtgc ggttcgcgca ccgggacgcg ctcgatgtgc agttccaccc
    27001 ccgcctttcc tttggccgac atctccgagg agctgcaggt gatcccggcc gcccccatgt
    27061 cctggatgcc cacgatcagg tcctgctcga taatctcgag ggtcgcctcg accagcagct
    27121 tttccgtgaa cgggtcgccg atctgcaccg aggggcgctt ctcctgcgat ttctcggaga
    27181 gttcctcgga ggcgaacgtc gcgccgtgga ttccgtcgcg gccggtcttc gacccgacaa
    27241 tcatgagcgc gttcccggcc ccgcgggcga ccgccgaagc catccggtcg gctcggacca
    27301 ggccgacggc catggcgttg accagcgggt tgcccgtgta ggcctcgtcg aaacagacct
    27361 cgccggcgac tgtcgggacg ccgaacgaat tgccgtagtc gccgatcccg cgcacgacgc
    27421 cgtcgacgag ataccgcacc cgcgggttgt ccgggagccc gaagcggagg gagtcaagcg
    27481 cggcgatcgg ccgcgcaccc atggtgaaaa tgtcgcgcag aattcccccc acgcccgtcg
    27541 ccgccccctg gtagggctcc accgccgagg ggtggttgtg cgactcgatc ttgaacacga
    27601 ccgccaaacc gtcgccgata tccaccgcgc cggcgttctc ttcgcccgcc ttggccaaga
    27661 gcgcctcgcc ctcccgcggg agtgtcttca agagcgcgat cgagttcttg tacgagcagt
    27721 gctccgacca catgaccgag aatatcccca gctccgtcca ggtcatctcc cgaccgagga
    27781 tctcccggat ccgctcgtgt tcctctttag tcagcccgtg ggccgcaacc atctccggag
    27841 tcacctccgg ctgctgatac gccatctggg ttctgtccct ttcgaccgat gtttctccgg
    27901 cgccgccgcc ggtgtgacag gccaatatat cttcccggcg tcccccggtc aacgtccccc
    27961 gcccctctcc aggtggggga atttgaacgc cgcggggcag cgggccgcca gcgggcaggc
    28021 gggacaggcg gggcggcgcg ccgtgcagac cgcccgcccg tgcaggatga ccatgtgcgt
    28081 ccagtcgatc cagtcgcgct gcggcaggat tttcatcagg tcgcgctcga tccgggccgc
    28141 atccgagtgc gccgtgagcc ccagccgccc gctcacccgg atcacgtggg tatcgaccac
    28201 caccccctcg gcgatgccgt acccggcccc aagcaccacc gaggcggtct tccgccccac
    28261 gccgcgcagc ctcaccagct caccgatcgt gcggggaacc tccccgccga actgctcgag
    28321 aatctgcagg gccgactcaa tgatcgcccg cgccttggtc gggccgtagt tgattgacgc
    28381 gatgtccgcc cgcaggtcgt cgacatcggc gttcgcgaac gcctcgatcg tcgggtactt
    28441 ccgaaacagc cggggggtca ccgcgttcac ccccttgtcg gtattctgct gcgacagaat
    28501 ggtcgccacg agcaactggt ggaccgagcc gtaagccagc gtggtgcacg cgccgggata
    28561 gtgccgcctg aggatgcgga cgatctccgc cgcccgctcc cggcgggcgg ccgcgccctc
    28621 gcgcggcatc agtcgccccc caggatgatg acgcggtcgc ggtcgttgag gtcgcggagg
    28681 agaacgaacg tgcggtagcg cgggtcgcgg gcggtgagcg cagcgacttt gtccgcctgg
    28741 tcgtggccga tttcgaacat gagccgtccg cccgggacga ggtgggccgg ggcctcctgc
    28801 acgaggaccc ggatcgcgtc aagcccctcg gggccggcgg tcatcgcgat cttcggatcc
    28861 gcccgcacct ccggcgccag cccggcgtaa tccggctccg cgatatacgg cgggttggag
    28921 acgatcaggt caaaccgctc gtcggcggcc accgccgaaa aaaaatccga cacccgcagg
    28981 tcgacgcggt cgcggacgcc gtgggcggcc gcgttttgcc gcgccaccgc gagcgcctcg
    29041 gccgatatgt ccaccgccgt cacccggcac gcgggtagtt cgagcgcaag ggtcaccgcg
    29101 atcacgcccg agccgacccc gacgtcgagc acgcgcggcg cggcgagacc tcggctcccc
    29161 accgaccgca ccgccgtctc gaccagcaat tccgtctccg gcgtcggcgc catgaccgcc
    29221 tcgctgaccg agaatttccg tccgtagaac caggcttcgc cgagaatgaa ctggagcggg
    29281 tggcgggtca ggcgcctttg gacaatggcg tcggcctgcg ccgccgccgc gtcggtgaga
    29341 aggtcgtccc cttcgagata gagccggagc cggtcgacac cgagcacgtg ttcgaaaatc
    29401 cactcgagct ccgcctccgc ctgttcgatt cccgccgccg ccagttgggc ggcgtaccgg
    29461 gccagcagcg gtcggagccg gttggtcatc cggcgcgccg ctccgccttg tgcgcccgcg
    29521 gggcccgccg ccgcacgatt ccggccgcca atccgccgaa atacgcggcc gccgccaggc
    29581 cgatgaacga ccagcagacg atcctcgtca gcgcagggga gaactggatg gaatgcggga
    29641 tcagatagat ccccagcatg actgcgaacc cgagtacgcc cagccagccg agcacccgcg
    29701 gcgggtacgc gtcgcggccc acccggcgcc gggtgagcga gccgaacgcg gcaaaaacga
    29761 ccagcaggag gtacacgaac agcagctgcg tcttgttgtc ggtggcgtcg gtgccgaacg
    29821 gcacgccctc ccaggtcgtc ccgaaagtga accagttcat gggaatgccg aacgggtagc
    29881 cgcccagaaa cgcgcacagg gcggcaatgc cgtaggcgcg cgccatcgga aacacgtccg
    29941 tgcccccgcc ggtcagccgc gccccgtgga gagcgccgat caccacgaaa tacaccgtcg
    30001 caaacatcag ggcaatgtgg gtgagcaaga gccaggtcgg cacatggccg acatatttca
    30061 tcacaaacgg ccgcccgccg tcgagaaaca ccgcacgctg cccgccgatc ttgtcgcgca
    30121 cctcgatgta gtacaggaac cggcccccct tgagcccggc cgtcatcagg tacccgtacc
    30181 gccctccggc cgagtccagc acctccagcg gcgtcgttcg gtagcggtac agcggggtcg
    30241 tctcatcctg cccgaaccgc gcgtgacgga gcaccggctg cacgctgccg tcgaacggcc
    30301 cccgaatcgt cgccgtgatg cgcgccgtgt cgacctcgta cgccttcggc accgtcatca
    30361 tctccagcgt gaacccgcgg tcggtgtgca cgtacggttc gggacggccg cgcgagttgg
    30421 tgcgggcgat gtaaagcagc gccgccgtga ggaccagcgc gagagcgaag cgggttatct
    30481 tcatagctgc gcccccgccg tctcctggag tttgagctgc tgctcctgct cgtgccgccg
    30541 aagttcgtcg atgagcaggt cgacgtcccc ggcgagcacc tggtcgaggc ggtagagcgt
    30601 gaggttgatg cggtggtcgg tgacgcgcga ctgcggaaaa ttgtacgtgc ggatcttggc
    30661 cgaccggtcg cccgtcgaaa tcatcgaccg ccgctcggcc gcgatcttcg cctcctgcgc
    30721 ggcgatctgc aggtccagca gccgggcccg aagcactttc agcgccttca ccttgttctt
    30781 caactgcgac ttctcgtctt ggcaggtgac caccaggccg gtggggaggt gggtgatgcg
    30841 gaccgccgaa tcggtcgtgt tgaccgactg ccctcccgga ccgctcgacc ggaacacatc
    30901 gaccttgatc tcgttttctt tgatctcgat atcgacttcc tccgcctccg ggagcacggc
    30961 caccgagacg gccgaggtgt ggatccgccc ctgcgtctcc gtttgcggca cccgctgcac
    31021 gcggtggacg ccggattcgt atttcatccg cccgtaggcg tcctcgccgt cgagggaaaa
    31081 gatgatttcc ttgaatcccc ccgagaggcc cgggtgcgag ctgagaatct ccagcttcca
    31141 tccccgggtg tcggcgtagc gctggtacat gcggaagatg tcgccgacaa acaggccggc
    31201 ctcgtcgccc ccggtgccgg cgcggatctc gatgatggcc gccttgtcgt cgttggggtt
    31261 cttgggcagg agtttgagtt tgagcgcccg ctcgacctcg tggatttggc tctcgtaggc
    31321 ggcgagctcc tcgcgcgcca gcgccatcag ctcgtcgtcc tcatcggcgc ggatcaccgc
    31381 ctgggcctcc tcgatcccgg cgcggaggcg gcggtagtcc cgcccgatcg cgaggacgtc
    31441 ctcgacccgg cggcgctccc gtcccagttc gatcatccgg cgctgatccg atgccgctcc
    31501 cggctcggcc atctcgcgat cgatggcggc caggcggttc tccaatttgg ccacaagatc
    31561 aagcacagct catcctcgcg ttactccaca ttctcggaaa caagggtacg gggaattgtc
    31621 cgccggcgca aggcggaatg tgcgggcgcc tacgacaccg gaatccgcgt cgccgctact
    31681 ttcgattccg tccgcccgag cgccgtctcg agcgccacga tcgccacctc gagctggtcg
    31741 aacgagggct cccgggtggt gatcgcctgc agccagagcc ccggctggat cagcacccgg
    31801 gtgaccttgt ggtcgcgcgt ccggcccgac agcttgagca gctcgtagga tcccccggcc
    31861 accagcggca gcagcgcgag gtgcaccgca aaccgggtcg ccagcgaggg cgggtggccg
    31921 gcgatcaccg cgaatagaga gtcggacacc gcatagatca ggatcgccgt gagcgccacg
    31981 atgaggataa acgaggtgcc gcagcggggg tggaagcgcg tgtagagcgc cgcccgcccg
    32041 ggcgtgaggt catcccccat ctcgtaggcg taaatcgatt tgtgctccgc cccgtggtac
    32101 tggaaaatgc gcttgaaatc cttcaggaac gagatcatcc agacatagcc gacaaacagc
    32161 gccagccgga ttcccccggc caggagattg aacccgaccg catctttctt gatcccgacc
    32221 agcgaggcca gcgcgagcgg gagaaagaaa aacaggccga tgccgagcgc gaaggcggcc
    32281 gccaccgaca gctctaacac cagcgcgttc gactttttat ccttcccggg cgccgccgcg
    32341 gcgccgttct tttccgcctc ggccgcctcg atttcccgca tggccacgtc cgccgagaaa
    32401 ttcagcgtcc tgatgccgac cacgagcatc tccgcaaacg agaccgttcc gcggatgatc
    32461 ggcgccccga gcaccctatg ccgcttggtg agagagaggt agggctcgca cttgacgaga
    32521 atctcgccgt tgggttttcg cacggcggtg gcgatgcgat cggccgagcg catcattacg
    32581 ccctcgatca cggcctgccc gcctacattc aagtccggca tggctctggt tggctcccgt
    32641 cgtggccagg gattcgcgcg cactgccgcc gcccgcctgt ccgccccgcc ggcggtctcg
    32701 ggcggccccg cgcggcccgc tcctgcgtta cttcgaatcc ttctggtact tcttgtactt
    32761 ctggatgaag cgctcgaccc ggccggccgt gtcaatcagg cgctgcttcc cagtgaaaaa
    32821 cggatggcag ttcgagcaga tttcgacact gatatttttc ttggtcgacc ggttcggata
    32881 ggtcgccccg cacgaacagg tgatcgtcac gtcgaatgtc ttcgggtgtc cttcagcttt
    32941 cacaactcgt tcctcaacgt tggctttggg ttcggccctc cggggtcgat cgcccgacgc
    33001 ccggagcctc ttaatatata cgcatccccc tgcctggcaa gatgaatctt cctgccggcg
    33061 cgctcgccgc gcccgcacgt acaacctcgc gcgatcgccc gcggttcccg cgcggcccgc
    33121 caggcgaatc ataagttcat gtcggataat aagattgacc agtagtaggt gccgtagacc
    33181 aagcggtcca cgcgcccgta gggcgcgcac agcgaaacca gctcgtcccg ctgaaagtag
    33241 ttcttcaaga tcacgaacgg ctcaccgttg tccagaaacc gccggcggaa attgttgccg
    33301 tgggcatcaa ccccggccgc cggcagcgac cgtccctcgg ccggcggatt gttatcgatc
    33361 atccagaggc gcccgcccct tctcaccagc cgtccgagca tcggaaaaaa tgtcgggaac
    33421 gtctctctcg gctggtgcga cagccagaac gccaggacca ccagatcgaa cgccccctct
    33481 gcgaacgggg ggtgttcaag gtcggcgatg agaaagtgcg ggggtcggcg gtactccttg
    33541 cggccggcct gggcgatcat ctcgggggcg atgtccgcgg ccgccaccag ggccgcccgc
    33601 tcggccagga tctgggtcca gtagcccgtg ccgcagccga ggtcgagcac ctcgcggccg
    33661 tcggccagtc cgcgcaggaa agcggcctcg tcgtcgatct cttttcggcg cgggggattg
    33721 tcgcggtagt agatctgttc gtactccggt gcgcgtcgcc ggtagtaccg ccgcgtctgc
    33781 tcgtcaccca cagcgcctcc ggagcaatcc ggccagcctg tgtccgagcg cgtcggcctc
    33841 ggccagggcc gccgccgaac cggccgcctc gcccgcctcg agcccccggg tgcggtaggt
    33901 gagcagcccc tcgttggtca cctcggccca tttgaagaac cccgcgatca cgcgccgggc
    33961 tccctcggtc cactgccgct ccccgctcac cagcaccatc gcccccggcc gcttgcgcgc
    34021 gatgagcttg aggaaaccgt ggtcgggatc ggccccgcga aaatccgccg gccgcacaca
    34081 gttgcatcgg tcgatgaaca gcttcgcctg gctcgacacc gtgtcgaaat gaatcgggct
    34141 cccgaacagc aggcagtcgc agcgcgccag ctcgcggtag agcggcgcca tgtcgtcatc
    34201 aaaaaaacaa aactggggtg tcggcgcctc gccgcacgcc tggcaaggga ggatgcgaag
    34261 atcgttgagc ttgacaaagg tcgtgcgcac ccgcgccgcg ccgcgcagac cgcggcggac
    34321 tgcgtccgcc gcctgccgca gaaggatgtc ggtcgaggct cccggcacgg gcgagcccga
    34381 tataaccagc agactaatca cagtcgcgcc gctccagttc ctcaagttcg ccgtacagcg
    34441 ccaggagcgc cgcttccacc tcctgcttgc gccgcgaagc ggcctcgagc gcttcccaat
    34501 cggtgcgcgg aatgcggcgg gcgatcccgt cctcgagttc ctccagctcc cgctcgtgat
    34561 cggcgatctt tgagcgcgtc gacttcaacg ccttcttcag gcgcgccttg acccgcgagg
    34621 cttccttgaa cgcctggtag acctcccgcg atttttcctt gtccggcctc ggcgcggcct
    34681 cgagcgcggc cgcggccgct tccgcctgct tctcttcgaa atggctgtag ccgccgccgt
    34741 agacgaaagc ggtcccgccg gccaggtgga tgatccggtc ggccacccgg tcgaggaagt
    34801 gccggtcgtg gctgacaatg aggcagctcc cgtcgaaccg cccgagcgct tcctcgagcg
    34861 cctcgcgcga gtcgatgtcg aggtggttgg tcggctcgtc gaaaatcagg aaattggccg
    34921 ggtggtagat gatccgcgcc agcgacagtt tcgtccgctc cccccccgac agcgcggaca
    34981 cgcgcttgaa gcagtcctcc ccggtgaatc cgaaacgggc gagaaacgac cgcatcgccc
    35041 cgacctcggc catcggatcc tcctcccaga tgttgtcgag caccgtggcc tcgagccgga
    35101 ggttgtccag gtcctggtcg aaatagacga cgtcgacatt cgccccgagg cggagctcgc
    35161 cgctcaccgg ggcgagctcg ccgatcagcg ctttgagcag cgtcgacttg cccgatccgt
    35221 tccggccgac cagcccgatc ttctcccccc ggtagagatc gaaactgaca ttccgcagga
    35281 ccggctccgc gccgtaggcc agcgtcaccg accgcacctc gagcacgtgg gcaaacgacc
    35341 gtcccgagga cctcaccgcg atactcggcc cggcgccctc gccccgcggc ggagggatgc
    35401 gcttgatccg cgacaggtac ttcagcttcg actgcgcctg cctcgtcttc tgcccggcca
    35461 tgttccgccg gatgaattcc tcgatgcgcc ggatctcttc ctgctggtgg cggtagtggt
    35521 gctcgtggag ccgcttgcgc tcggcccgct cgacgaggta cttctcgaat ccgccgacat
    35581 actggtgaat tttcccgaac tggatttccc acaccttgtc gaccgtgcgg ctgaggaagc
    35641 gccggtcgtg cgagacgatc acgcaggcct tctccagctt gcgcaggtac tcctccagcc
    35701 agacggtcga ttcgatgtcg aggtggttgg tcggctcgtc gagcaacagg agattgccgc
    35761 atccggccag ggccatggcc agccccgcgc ggttcttctc gccgcccgag aaggtggcca
    35821 gccggtcgcg gtggcgggcg cgctcgaacc cgaggccctc gagaatgagg cggacctcgc
    35881 tctcgaatcc gaatccgccc gcggcctcga actcgtgctg cacctgcccg agccgctcga
    35941 gccggcgcgc gtcgtgggga tcgtccgcca ggtgctcctc gagttcggtg atctcgcacc
    36001 gcatctgggc gagatcccgg cgggccgagg ccaccaacgc gaacagctcc cagtcgagca
    36061 ccgaggtctt gtcctgcggc acgtaatcga tcacgcagtg cttcgcgagc gtcaccgtcc
    36121 cggcgtcggc cgccacccgc ccggtgagca gctcaaagag cgtcgtcttg ccgatcccgt
    36181 tgcgcccgac caggccgatc cggtcggcgg ccgtgatggt gaacgagacg gcgtcgaaca
    36241 gcacctggtc ctggtacttc ttggaaacac cctccgcggc gagcagcgtc atcccggctc
    36301 ctcggggggc gcgccccgcg ccagcagcat ctcggccagc aggagcccgg ccgcgatcca
    36361 caggaagagc tgccacagct cccgcccgta gcgggactca gcgatgagac cggcgagatt
    36421 cccctcggcc gccagctcgc ggtgctccgg cgcgccgacc gcctgcgcga accggtcgac
    36481 atccgccgcc gccaggtcgc cctcggccgg gtcgatgttg accgcgaacc ggtcgacctc
    36541 ctgcccggcg taccgcacct ggtagatccc cggccgctcg accggctgcg cccgcaccag
    36601 cacccttccc tccgattcct ccggcgacag gtagtagcgg ctgctgtccg gggcgatcag
    36661 ctccagcgac ccggagatcg cccccctgag ggagatcacc cgcggcgtgc tcgcccgggc
    36721 gtacagcttc aggtcgtagc tcgagaggtc ggcggcgagg tactcggcca cccgcgatac
    36781 gaaggggacg aagaaggcgt ggccgaccat gtcggagtac gccggcgcca tcggcccggc
    36841 gaacgtcacc acccgtccct cgccgtaccg ggcctccacc agcgccgggc ggccgccgga
    36901 gaacgtcgcg atctccgccg cctccccgac cgtgtgcagc ctgggcagcg tgtagaattt
    36961 caggtcgggg agtttctccg cgaacgaaaa cacggagaaa atcgggtgct ccttgaccac
    37021 cgactccagc gtgtagtacc cggcgcgcga gaagtccgtc cgcgccgcct cgtcgatcac
    37081 cacccccgcg atgtcggccc aggtccggtt gaacgaggcc gcatcggcgc cgccgccgta
    37141 ggtgagaaag agcgaggtcc cccgccgcac cgccgccttg atccgcccga cataggcatc
    37201 cgcgatcgcc ggcgtccccg gcagcatgat cacgtcgtac tcggccagat caaccgtgcc
    37261 gagatcctcc ggccgcgcct gcttcaccga ccagtactgg gcgaggtcgc cggagggggt
    37321 gagcgcgaga ctgatgaact gggccgccgg atcccccgcg accatgagca ccgtgaactg
    37381 ctccgggatg cggaaggtga agtagtagcg gttgtccgag gggaaccggt cgtcggacag
    37441 ctccacccag cccgagtgga acccgccgcg ggcgaccgac tgggtgaacc gcaccgcctc
    37501 gtcgcccccc gccggcgccg ccgcgtccgt ctgcgccacc cgccggccgt cgagatagag
    37561 cgaggtgatc aggtcggtcc g
//
LOCUS       DAOPOE010000018        37115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1505_length_37115_cov_5.562250, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000018 DAOPOE010000000
VERSION     DAOPOE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37115
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_37115_cov_5.562250"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PKM94_04190"
     CDS             <1..1069
                     /locus_tag="PKM94_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD65871.1"
                     /translation="GYGGQRFAYDRTAIHVNEWEEYYFQPVYYPVYHPWTLAGNVYID
                     YPFGATVYINGIYWGCTPLYLPRVYVGWHTITVIDHWGYCWESDIHVSRYHTLVLDHH
                     HIRPAPHVKSKYREVVVAGYRDPVKNGYPDYVARTKQIASAKGVRTEEVAVQRPSGER
                     TVEKVAIGPKTHVRGATNLVKTDRGFETAGLVPDWAESSYSRSSKKFTAGSEGRSTKP
                     SDGYQGPGSGYASGKRDVVVGKDGQTESKNQGGAVTQERERTSSEYYQRKSGSDAGRS
                     GFKENAQRREVEVNQGRSKGSSGSRPSYKPSDGGSSKGKGATVAPPSKPSGGSSPSGK
                     SSGGSGTVKSGGGGGKGKGGR"
     gene            1136..1540
                     /locus_tag="PKM94_04195"
     CDS             1136..1540
                     /locus_tag="PKM94_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65872.1"
                     /translation="MIKRIFISLVCAGLLFSLGGNAGAVEKKSKDDKAKTETKAGAVK
                     KDAPVKKDPAAAKKPATKQFDTFVDENGNGIDDRRENLKNKAVAESQAARAKAQSVSA
                     KQAESKKADGSKKADSVKKVEPKKATDKKDGK"
     gene            1759..2817
                     /gene="hrcA"
                     /locus_tag="PKM94_04200"
     CDS             1759..2817
                     /gene="hrcA"
                     /locus_tag="PKM94_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOD65873.1"
                     /translation="MAFENLTEREKQVLSNLIEHYIRSADPVGSRVIAHKFRMGVSSA
                     TIRNTLQDLEELGLVRQPHTSAGRIPTDLGYRVYVDYLLKPEDLTESEKERIKDVIVS
                     HGRGLDDILGQTCRVLSDITNQLGVSIAPRFDRGVLRRIELISLSQGRIMAVVVVESG
                     LARSVIVEVETSIDEASLREVEQVLNERLAGLSLGEIRTTISKRLADHTGSGRLVKLI
                     IDSRDRIWADDPAGAIHLAGVERLLSQPEFSEIDRITRIVRLLEDNRTLKEFLSSAYE
                     EGLFITIGRENKFDEILNCSLVTSSYKVGSIAGTIGIIGPTRMEYGKLVSIVDYTARA
                     ITEVLSGMSSKEGESKDA"
     gene            2810..3442
                     /locus_tag="PKM94_04205"
     CDS             2810..3442
                     /locus_tag="PKM94_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD65874.1"
                     /translation="MRKKDIEPEDPVAPGGQPCESVERSGSGEAPPAAGVENGDAEPG
                     PPPGEIPEEEQLRRRIAELEDRLLRTAADFDNYRKRQARQYDELVKTAADRVLSDLLD
                     IVDNFRRALDAAAGEGNMDAYRQGTELIYGQMTELLARYDVTPIEAVGRPFDPNVHEA
                     LLRVPSAEHPAGTVAVEVSRGYRRGERVLRYAKVGVSTGAPEAPDEGKEN"
     gene            3474..5396
                     /gene="dnaK"
                     /locus_tag="PKM94_04210"
     CDS             3474..5396
                     /gene="dnaK"
                     /locus_tag="PKM94_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD65875.1"
                     /translation="MGKVIGIDLGTTNSVVAVLEGHEPAVIPNQEGSRITPSVVAFTR
                     DGERLVGAPAKRQAVTNPENTISSIKRFMGRRISEVQSEEKLVPYKIVASAEGMVQVE
                     AAGSRYTPPEISAMILRSLKQAAENYLGHEVTQAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTAASLAYGLQKNKNQKIAVYDLGGGTFDISILEIGDGVFEVRATNGDT
                     HLGGDDFDQAVIDWLADEFRRQNGIDLRKDRMALQRLKEAAEKAKIELSSTMQTNINL
                     PFITADQNGPKHLDLTLSRAKLEQLTDQLTQRTVEPCRRAVQDAKLSFSDIDEVILVG
                     GMTRMPRIVALVKELFGREPHKGVNPDEVVALGAAIQGGILGGEMSEKDIVLLDVTPL
                     SLGIETLGGVMTRLIERNTTIPTSKAQIFSTAEDSQTAVSIHVLQGERPMAQDNRTLG
                     RFDLVGIPPAPRGIPQIEVKFDIDANGIVHVSAKDRATGKEQSIRIEASSGLSEQEID
                     RMVKDAERFAADDKRREELIKARNEADQLAYSTEKTLKEAGDKISAEERSRVESAIRK
                     LREAIPRESLQDITSAKDELIAASHKLAEEMYRQAQSQGSPGGAPAGEAPAGGAPGGE
                     GAVDADFEVVDDDKKK"
     gene            5408..6580
                     /gene="dnaJ"
                     /locus_tag="PKM94_04215"
     CDS             5408..6580
                     /gene="dnaJ"
                     /locus_tag="PKM94_04215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOD65876.1"
                     /translation="MNKRDYYEVLAVDRGATEEQIKSAYRKLALKYHPDRNPGNQEAE
                     EKFKEATEAYEVLKDPRKRQTYDQFGHAGVGAGGPGFGGFGGGFEGFDLGDALRAFMR
                     DFGGSGSIFDDLFGMGMGSGTRQRRTVRGEDLRVRIPLALEEINTGLEKSIRVKRLVT
                     CDACDGTGVAAGSSKKTCPQCKGRGQVRTVTRTFLGTVQQVSTCSMCRGSGEIIADPC
                     PSCGGEGRVRGESTVKINIPAGVASGNYLAVEGMGNAAPAGGPPGDLIAVFDEQEHET
                     FERHGDNVVMELPVSFATAALGGTVEVPTLDGSAKLKVPPGTQPGKVLKLKGKGIPHL
                     HTGGRGDQLVRIHVWVPTRLEAREREMLEQLARCESFKAPQGGKSFLSKLRETLGV"
     gene            6594..7475
                     /locus_tag="PKM94_04220"
     CDS             6594..7475
                     /locus_tag="PKM94_04220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOD65877.1"
                     /translation="MAATSPESIYEIRVRVPRAQVDALSDFIVERISAGLVLEEEEGS
                     PETRVLFYANPEAVAAQMAMVRGYVVQLLGADVDPGRLVTATEIRNRAWEEEYRRSVQ
                     PVVIGDIVIRPPWAPAPIATPYDIVIEPKMAFGTGTHETTRSCLRIIRQHLQPGGRVL
                     DLGCGSGILSILADKMGAGYIKAIDYDVTAIDNCRENFRRNAVAAPHDILFGSIGKCR
                     GDRPYDLAVANIIKSTILEMLPRLVELTGRPGHLVLSGLLEQDVPEVAAGLDRLGAAD
                     RAVLPDNEWRTITITFA"
     gene            7479..8237
                     /locus_tag="PKM94_04225"
     CDS             7479..8237
                     /locus_tag="PKM94_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOD65878.1"
                     /translation="MPTPLPIFYAPPEDLSGDAVVLRGDEAHHALRVLRLGIGDRLLV
                     IDGLGAARRGEIVEAGARGATVTVRVRDTIRNFGEPAVRLTLAAGLSAGAKFDAVVQR
                     GTELGVQRFVPILGEKSKVRMEDAGRAAARVRRLEKVALAAVKQCRRSYRPEIALPTG
                     LESFLAYTDPEALNLVFHAAPGGKPLSAQAIPPGVSRVSLLVGPEAGFSEAEAGRAIA
                     AGYAPISLGPRILRSETAGPVVCALVMHALGELS"
     gene            8265..9047
                     /locus_tag="PKM94_04230"
     CDS             8265..9047
                     /locus_tag="PKM94_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOD65879.1"
                     /translation="MNTLILIWIGAVGLAIGSFLNVVIYRLPLKKRFGLGRSICPKCG
                     TQLRWYHNIPLASWAALRGKCAFCRQPISWRYPLVELMTAGAYVYFWAYLGFSPMFFV
                     WAALVSALIVIIFVDLDHQIIPDSITLSGMAAGLAASFLPNGLGIVQSLIGLVVGGGS
                     LYLIAVLGEALFRKEAMGGGDIKMAAMLGAFLGWQKVLLVFLAAAVIGLVVGVIAMLI
                     SSKMRRDRMIPFGPYLAVAAVISGLYGDRIIGFYLTRIVGLN"
     gene            9084..10922
                     /locus_tag="PKM94_04235"
     CDS             9084..10922
                     /locus_tag="PKM94_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD65880.1"
                     /translation="MRPGRHKFQTEVQLGILALVFLLLFLNFTSNFLLYAARSSKRAA
                     VAAHLAEAGQAVLRLMAERGAEGATGEGQSEIMRRFRLESLWILPAAPESRAVEDRRR
                     WLAAVADRFPPDRVPGIVRQVVAGKPGRLNRGEGAEYFLLQAAPGPGGAKLLLVSRRE
                     PELARLDDVYRLALVMSLIALVATAAVYVILARLIFAPFRKAKEEARTAGRTVDESDD
                     DVEVLLNDYRRMIAELREKERELLRLNLEISRRAGSLEQFNRYLLASMPSGIVTVDRA
                     GRIQTINAAAERILGLREGEARGRFHAELMPRELAEAIAGPLVDEAGGEYREVTPAGL
                     PDTPVLGIAASTVWDGEGEPIGAALLINDATEITQLREQLERRLRLAALGEMSGGLAH
                     QLRNSLGAIAGYLNLLKRRLGKRGVEEPAVAELADEAAQAESLVRRFLEFARPLQADL
                     QAVPLRDLIGQAAASFATREAYPRVEIAVGEIPEVVIPADPLLLRQALLNLIENAANA
                     YSGAAGKIEIDAAAGNGETAIRVRDRACGIAPENVAKIFTPFYSSRPSGTGLGLALVS
                     KIVDLHKGRIEVDSRVGRGSTFTITLPVGAPQPAAAEGPAEMRVRG"
     gene            11119..11679
                     /gene="efp"
                     /locus_tag="PKM94_04240"
     CDS             11119..11679
                     /gene="efp"
                     /locus_tag="PKM94_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOD65881.1"
                     /translation="MYSPSDFRKGLRIIVDEQPFYIVDYQHVKMGRGRANIRTRLKHM
                     KTGAVIERVFGSNDTFKAPDTQERKMQYLYENAGEFSFMDTESFEQVVILADSLGDAK
                     WYLVENSEYRVFFLDGQPISVDLPASVTLEVIYTEPAARGDTVSNVTKPAKLSTGLEV
                     KVPPFVKEGDMVKVDTRTGEYLERAN"
     gene            11679..12338
                     /gene="rnhC"
                     /locus_tag="PKM94_04245"
     CDS             11679..12338
                     /gene="rnhC"
                     /locus_tag="PKM94_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOD65882.1"
                     /translation="MAAGDRRVIGVDESGKGDFFGPLVVAALAAPDSALADLKGLGVR
                     DGKGIADRRLLTIDEQLRRAYPFAVEIMPPEEYNRAYEKIRNLNRLLADLHARAIDRL
                     LRDHPADLVVVDQFGKTALVAEAVAFHGHSVALEQRFRAEEIPQVAAASIIARAEFIR
                     QLQTLSREMGVELPKGAAAIVDRAGAALVRQHGRDILRKTAKIHFKNYQRVINPSLFP
                     S"
     gene            12498..14081
                     /locus_tag="PKM94_04250"
     CDS             12498..14081
                     /locus_tag="PKM94_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD65883.1"
                     /translation="MALPWAPFGAALFMLALLVLLRLYGKYLADQYREQFRNLVVGVG
                     LFGLVAVLYLGEATGATAAVPFISQPPFLEVSAAILAITGAVLTASGASVWLPARRTG
                     KILHDVPSEPLRREAEIVRAQLQDALALGAPLADSFTAIARVLKAALGGELSSLAMAY
                     GEWDIRRYTAGGNGTHLTEKSLAREAAGLPQENDSPPPDRPFVTVDFERFLEPALAAV
                     LRRQQLAAALLVPLGDPGGAAMLVAAARSAEEFGERERYLAAQVRFPLRLAVAVERER
                     DRLARGMKRSEALARLSAQAAARLGDIDRLFAEAADVLRRSTGAAAVRIALFEDDGQF
                     LRSRAAAAAHPVENAVPATGYLIRSLMPLHQSVQATGEALLISQAEAAERMSAAEAQQ
                     IYQSDLSTALLSPITAGTETLGIIALADRRGSRRFGFTDDDRTFAAAVAAVLAQAVRL
                     HETPWGALTWRDRESGEDADRRAELRGRIRSSLTGILGSVEMLRARPVGPEAERFVEI
                     IDRSARRIETIFSEETPAR"
     gene            14118..16196
                     /locus_tag="PKM94_04255"
     CDS             14118..16196
                     /locus_tag="PKM94_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65884.1"
                     /translation="MDVPTYKRCAARGPEAAPPELPAAAALYTGGGIPQLFRRLGTAE
                     GADAWLYVPAGTLDSLVPTTPATEGQFASAEGLTVDDSGRFCPLVVGGAVRGFLSGMD
                     RPDGAALKAAVLDWLRTREIATQATLLPAQAELAAALVACDLSPERLLRNALNLLVEQ
                     VPSGLAAVYGESEGVYGLRTAVGDIAMWDMLEGTLPHATVGRWMEGLAVGRHFLPAGF
                     LPERPMLLDAAPDFLFVHEGLRCSRPRSFVIMPVPGDIAREAGERLLTIARFIAALDA
                     DGLSITEDLINVCGQFRFACDQGRPGRELLENLFEVLQRRMGLSRIVLAAADGGGAAA
                     VGRPEMPPSVRAESSPGIAAAIQAAARGGRSFSPDVSALGLLDDDEAKRYYLDNVRSE
                     YCVRPRDAGCGETLVAFGHPETGLRLRQFERLLDSTAMLLDLYYQLEGRLNASPGAAD
                     AQGKPERRWATASAMAEGHFHAVYDTLSGLLGKTEVAAAIGGAAAQTGHSPAGEALAR
                     VVRSLDHLRALVRDTASAADEPVAVERALAELPGMLEGKAHHLEDLRGLALTVAIGPP
                     SGRSFSILRRDLYDHLYPALLAVMEAAVRSGEVRIRAATHRGADAVEVTCPAPLLGRR
                     SIADVMQVWTGEAGEREGGGRTLFDAGRYVWVCDARGRDPQRAWLAIRHDINTLRPDR
                     PVDRHDREVG"
     gene            16196..18331
                     /locus_tag="PKM94_04260"
     CDS             16196..18331
                     /locus_tag="PKM94_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD65885.1"
                     /translation="MRTSGILIQKYYHVRTGHKLAGIIAQVDTLSLMAARQLGCDFCA
                     VFYRTAGNRTLIPVSYQHGSHLSIRNLELLEKAWGEAEVEKTPPERGLRDLPARASAA
                     DAFGAANGFACRYLLPVHEDGRLCAVVIGYWFERPGAIAGEAERMLDLVGSVLRAALS
                     IADQLLVVENYSTRLSELLPVFEAPLEETKFSALVSATLGRAARICPTAYVVLLARDR
                     RTGRIGCAESYGDRKPSSELCDFILRAAEPLTQVEAAGDEDDTRRFRCRDLARADTLG
                     LTDLVVLPVAPGDELYYLLAVGATGEHGLSANDRELLSVFAVFAQTVLRNALLFRRLQ
                     RANRLLERSSDRLADAEAMAALTDLTSGVAHDFNNIFGGIMGRVQLLRLRARELPQFN
                     DELAKVERLVAEGAETVRRLQEFATSGRAKDVQPVDLVALVQKTLQPAQARWRELAVS
                     KSIRIEFRPETEAAVIEGCAEDLATVLRKLLENAVEHAPEGSTVAVALAADASDYRLQ
                     VCDRGKGIPPEAGKKIFYPFFTTKTERGAGLGLAIVHGIVSRHGGKVGYECTPDAGTV
                     FTAAFKRPATVAEISEVTTRRKASAAGLRILVVDDDDQIREVLRDMLVIAGHQAVACA
                     NGFEALETVNREPFDIMITDLGMPGMSGLDLAGAVHEQAPKMPIAMITGWGTQLDQRE
                     IAAKGVRAVLSKPFHLKDIRALVDELAGA"
     regulatory      18397..18520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            18602..19705
                     /gene="ribD"
                     /locus_tag="PKM94_04265"
     CDS             18602..19705
                     /gene="ribD"
                     /locus_tag="PKM94_04265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOD65886.1"
                     /translation="MADETDIRYMQRALELAARGRFRTAPNLMVGAVIVRQGRIIGEG
                     YHRKAGSDHAEIAAIRNAVEPVAGSTVYVTLEPCCHTGRTGPCTAALIEHGVARVVFA
                     ATDPDPRVRGKGARRLRQAGIRVEAGILREQAVRLNEVFFAFHRTGRPFIILKTAQTL
                     DGRIGAGNGDSKWISSPASRRMAHELRAEADAIVVGGGTVRRDNPALTVRHVTGPDPY
                     RVILSASLRFPKSCRLLDDNADARTIIATMAENVERFARTRRGRSLTFWSIRRRRDGQ
                     LDVADLVRKAGQFGLRSLLVEGGSRVATSFLKAGLVDKYVAFVAPKLLGTGIDAVGDL
                     GIASIDRALTFARTEILTCGPDIVVIGYPKRRK"
     gene            19707..20342
                     /locus_tag="PKM94_04270"
     CDS             19707..20342
                     /locus_tag="PKM94_04270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOD65887.1"
                     /translation="MFTGLVEDVGTIADIGLRGNYRVLSIVSVLADEHLRIGDSVNCD
                     GACLTVVRVGAGTFTVEASPETAARTILNDYRRGDRLNLERALRAGDRLGGHFVLGHV
                     DTTGTIEHARSAGESREIAVTFDPAFDALVVEKGSIALDGISLTVNAVRSAWLAVSLI
                     PHSLARTTAGGYQAGRRVNIEFDMIGKYIIKIRQSTGSNTLTLDKLRQSGW"
     gene            20346..21572
                     /locus_tag="PKM94_04275"
     CDS             20346..21572
                     /locus_tag="PKM94_04275"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOD65888.1"
                     /translation="MADEQIFNTVEEAIQEIHAGRCVIVADDEDRENEGDLIMAAEKV
                     TPEWVNFFAKHGRGLICVCMPEERIARLALHPMVDRNTALHGTRFTVSVDAVEGTTTG
                     ISAADRAKTVRVLANPASRPEDLARPGHIFPIQAVTGGVLKRAGHTETAVDLARLAGL
                     NPVGMLCEIMDDDGSMARVPRLAQFAREHGLAFITVRDLIAYRRRTEKNVERVETVDF
                     PTEYGDFRLHLYKSKLDDHYHVALVKGEVAGKKDVLVRVHSSCVTGDIFGSCRCDCGQ
                     QLHAAMRQVDREGCGVVLYMTQEGRGIGFANKIRAYRLQEDGQDTVEANESLGFKPDL
                     REYGVGAQILVDLGLSSIRLLTNNPKKVIGLEGYGLEITERVPLEFAPGKYNRRYLEA
                     KRDKLGHFLSLKRMGA"
     gene            21576..22046
                     /gene="ribE"
                     /locus_tag="PKM94_04280"
     CDS             21576..22046
                     /gene="ribE"
                     /locus_tag="PKM94_04280"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOD65889.1"
                     /translation="MAYKTYEGKLEAAGFRFGLVVSRFNNFLTDKLLEGALDCLMRHG
                     ASEDKIAVAYCPGGFEVPPVAARMVRSGKYDAVVCLGAVIRGDTPHFEYIAAESAKGI
                     ARLAMEAGIPVIYGVVTADTLEQAIERSGTKAGNKGWHAAEAALEMVNLYRAIE"
     gene            22090..22527
                     /gene="nusB"
                     /locus_tag="PKM94_04285"
     CDS             22090..22527
                     /gene="nusB"
                     /locus_tag="PKM94_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD65890.1"
                     /translation="MRPFEQPRRQARELVLQALYATEFGSARPEEALADLLADQPLAE
                     GATAFAQRLFASTRRRVEWANAAIAELAVNWNINRIAAVDRVILRMALVELDEFPDTP
                     VKVVLNEAIELAKKFSTAESSAFVNGILDAWVKRNDRGTGPGT"
     gene            complement(22558..23589)
                     /locus_tag="PKM94_04290"
     CDS             complement(22558..23589)
                     /locus_tag="PKM94_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65891.1"
                     /translation="MYNGSGAKRGHAIALFSGGLDSALAILLILRQNIEVTALTFMTH
                     FGCDLGDRSSCGSNPYPTAEKFGFNVKLMHLGQKFIDIVENPRFGRGKNMNPCTDCRI
                     LMLTEGRTFMEMVNADFIITGEVVGQRPMSQIKPKLRLIEEETGLAGRLLRPLSAKLL
                     PPTIPERTGLVDRNLLEGIEGRSRKRQLELAAAFGLEDYPTPAAGCLLTDVGYSNRLR
                     DLMAHTDRLTFDELNLLKYGRHFRLAPDAKIVVGRDEEDNRHLLALKRPQHVHLEALD
                     TNSPVTLLAGRDRSEEILRTAAAITARYSDAKHAAAVAVTIFEEGAAPRAISVAPAAD
                     ADIQLLILK"
     gene            23745..24563
                     /locus_tag="PKM94_04295"
     CDS             23745..24563
                     /locus_tag="PKM94_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD65892.1"
                     /translation="MWRNRRWERKAAILRAINSWLKHFAFELQRMFFFSLRTSAALFQ
                     RPFYGTETIEQMYNVGVGSLFLVILTGMFAGQGLAYAFAVELAELGAKNYLGRIMAIA
                     VVRELGPLLAALMIAARVSSGITAELGAMKSSHQIDAMVAFGVDPRKKLIVPRIIALL
                     VMVPVLTVVTDVLAILGGYIVGTLVAHLAPSIYWAAVKERLVFGNLFIGLLKPVVFSV
                     VIALVSTYKGFTAEGGTKGVGRATTESVMISSITILVVNFFITRVVFATIKGWL"
     gene            24560..25330
                     /locus_tag="PKM94_04300"
     CDS             24560..25330
                     /locus_tag="PKM94_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD65893.1"
                     /translation="MIALRSVHFAYADRPILRDVSFSLAEGESMVILGPSGSGKSTIL
                     RLILGLECPQRGDVIIDGESICRMKESRKIDVRKKIGMVFQAGALFDSLTVGENVAYY
                     LLEHTRLPWDEVDRRAREMLGFVGLDADEIIDKLPEELSGGMQRRVAIGRALLSTNPR
                     IMLYDEPTTGLDPRSTEQVLKLIQRLNADKGTASVVVTHEIADALAVADRFVLISAGE
                     VAFDGTFEALRDSADPRVLDFLAPFRESFAEVSRKRFI"
     gene            25388..26323
                     /locus_tag="PKM94_04305"
     CDS             25388..26323
                     /locus_tag="PKM94_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOD65894.1"
                     /translation="MRSTVGVKWGGLKVGILIMLAILVLLWASLSGGGTSIFSAKFTY
                     HSYFKNVSGMVAGAPVWMAGVEVGNVRRIEFVNLDSLRRVLITFTVKESVHKMVTSDA
                     RVQLGTIGFLGDKYLEVIPGGIGGPVLPEGSLIPPQDVGSAEAVFAAAENAIGEAEAL
                     AANLDTLLARANAGVGTLGMLATNQELYVQMTRLASEMADLARGLQANQKELLTSVTR
                     AANTMADVGEKLDDSTGTVGRLVTDTMLYANLTALTSQLDSTLTKINRAEGSLGLLVN
                     DTTLYVEIAALLARSNRLVKDIQDDPKKYLGFSIF"
     gene            26335..26967
                     /locus_tag="PKM94_04310"
     CDS             26335..26967
                     /locus_tag="PKM94_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65895.1"
                     /translation="MRRCRILVVLAALSVLAVGGCSKSEQPAPNPDRPQVVPDRVIAV
                     PEGFGVIRLITGPYSPEIVSRGFYHLRGPIPLQPFELGLHQYALMDSIANYEVLRYRE
                     FDGFESVLDSVPPGRYTLLVYVSRLGRAETKLDTVVSPDGDTTITDPVKSMPPYDYYF
                     RPVLVDSIVVADGQVSTIDLSLSLEKWGRPVQTDAGWEAPRARYLLYSPR"
     gene            27119..27421
                     /locus_tag="PKM94_04315"
     CDS             27119..27421
                     /locus_tag="PKM94_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65896.1"
                     /translation="MPRYLIEVPHEEDTVACARVVKVFLETGSHFLTHADWGCEDGEH
                     KAWITVETDTREAARSVVPPAFRSQAKVTQLTYFTMEEIDAILERHGRGARDGSGN"
     gene            complement(27443..29719)
                     /locus_tag="PKM94_04320"
     CDS             complement(27443..29719)
                     /locus_tag="PKM94_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD65897.1"
                     /translation="MNLEQVLDLLRSDKDISENVTHWRSFDARPARYVDFPGALDGRL
                     IGALKTRGIDRLYTHQGEAVAHVAAGRDVVVVTPTASGKTLCYNLPVLDRIMREPESR
                     ALYLFPTKALSQDQLSELYDLIQKLGVDIGTYTFDGDTPQTARRRIRAAGHIVITNPD
                     MLHAGILPHHTKWIKLFENLKYVVIDEVHQYRGIFGSHLANVITRLRRICRFYGSDPQ
                     FICCSATIANPAELTARIIGRPVELVDNNGAPAGEKHFLIYNPPVVNKQLGIRRSALN
                     EAARLGALFLRQRIQTIIFAHYRLYVEVLLTYLQRELQGDFGRGVVIAGYRGGYLPNE
                     RRAIEQGLRAGRIHGVVSTNALELGLDIGSLDVSIIVGYPGTMASLWQQAGRAGRRSK
                     TSLTVMIANSTAINQFLCAEPEYIFGRTPESGIIDPNNLIIRSNHLKCAAFELPFEEH
                     EYARDDGTREILEYLEDAKIVRHAGARYHWSSEIYPAQEVSLRSASPENFVILNESNR
                     SEVIGEVDFFSAPIFLHPQAIYLHRANQYQVTRLDWEGRKAYVKEVSVDYYTDAETKT
                     DLKVLDVDRTRRFGEADISCGEVTVTSVTVLFKKIKFETHENVGWGKLTMPEIEMHTH
                     AVWYSFAGDVAFRVGVGGEDFGGALRGLANILGKIAPLWVMCDARDLRAVSQVRAPFT
                     ECPTIFLYENIPGGVGLAEKLFTEHERLCEAVYHRLRGCGCEAGCPACVGPSMEIGAG
                     GKAGALRLLEYMLATAPV"
     gene            complement(29782..30324)
                     /locus_tag="PKM94_04325"
     CDS             complement(29782..30324)
                     /locus_tag="PKM94_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HOD65898.1"
                     /translation="MAILRSKIVVVPLGEVDPMMVNRLAADIGPVFNRSVDILKGMKM
                     PSEALNVVRSQYYVTVLLAKLERVKANSREKVIAVCEEDLYLPDQDTLLGHADIVAGT
                     SVVSLYQLRQEFYGLPEDEKKVYPRLYKEALHQLAHLFELRECRNPRCVNYYSQMMLD
                     IDNKGRKFCDVCRRKLTGVV"
     gene            complement(30406..30597)
                     /locus_tag="PKM94_04330"
     CDS             complement(30406..30597)
                     /locus_tag="PKM94_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65899.1"
                     /translation="MNGPKDAHHRPFLGMYFKCCRVYSRIYLNREGSAFVGWCPKCAG
                     QVRVGVSPTGRRDRFFTAE"
     gene            complement(30677..31381)
                     /gene="deoC"
                     /locus_tag="PKM94_04335"
     CDS             complement(30677..31381)
                     /gene="deoC"
                     /locus_tag="PKM94_04335"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOD65900.1"
                     /translation="MSDPLLHELHRRFDHAALKPDVTAADIGRLCAEARAHEFCGVAV
                     NPVWIPLCGDHLRGTGVKVIGVAAFPLGANRTDVKVEEAVKGAADGAREIDLVANIGW
                     LVEGRCADVAREISAVRRALPYNVLLKVIIEAGLLPASRWAEAAQAVIDGGAQFVKTG
                     TGFFGGATVEQVRALSRAAGGRIEVKASGGIRTLADARAMLAAGASRLGSSASVAIMR
                     ELASPEPGGDTAAGSH"
     gene            complement(31378..32562)
                     /locus_tag="PKM94_04340"
     CDS             complement(31378..32562)
                     /locus_tag="PKM94_04340"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOD65901.1"
                     /translation="MYIRRAIILVIDACGVGALPDAAAYDDVGAATVPHCAAAVGGLD
                     LPNCRRLGLGNIVPIAGVPPVAAPAAAFGKMAEQSAGKDSTAGHWEIAGLITPVPFPT
                     YPHGFPPSLVREFELQAGVRTIGNIPASGTAIIEQFGEEHLRSGAVILYTSADSVWQM
                     AAHEERYPLAEQYRYCEAARELLTGEHAVARVIARPFIGSPGRFVRTAGRRDFSLPPG
                     GPTLLDLMVAAGRPTYAVGKIWDLFAARGITSHVKPEGNAAGMEATIELVRGDRDHGL
                     IFTNLVDFDQAWGHRRDPANFALALEDFDRRLGALLDALREDDMLIITADHGCDPTLA
                     RHTDHTREYVPLLAYGRRVKAGVNLGARATFADVGATVAELFRLPGSLAGNSFAGEVG
                     LR"
     gene            complement(32698..33546)
                     /locus_tag="PKM94_04345"
     CDS             complement(32698..33546)
                     /locus_tag="PKM94_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65902.1"
                     /translation="MTRLTACLSAVILAAVLALAACQQGEKTVLLRYKFAPGLQLVYN
                     QDMKRNVLVARGDSIIEKSSTAFKVRVMQTIEEVRDENTALMREVDSWSYTVPSREDT
                     LKRDSVSMERTMRILMQSDGNVLDVKFDDSDDLPSQNYIKHFMEQGMPVFPPGEVSPG
                     FSWTQTAKVLLPNENMEASTTYEVVAFVREAGYDCALLNVDGNLVIPVEPNPKDSVQR
                     FGVDRIRTTGKMYFAFREGMVVLQRERWIIQGDYTRVKKSDTATYDVRIEADVDYALE
                     SRKITP"
     gene            complement(33574..34152)
                     /locus_tag="PKM94_04350"
     CDS             complement(33574..34152)
                     /locus_tag="PKM94_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD65903.1"
                     /translation="MTCSDVVARLDDFLDGELSAEAAGRVRDHLADCAACRREHDLAV
                     QVRGLLGSAAAPDPGDEYFAESESHILARTVLAGDPEAVRRGGGEGAAPRSSTALLRA
                     LVSVAASLAILFAAIYLGSSRELLISRTIGPEIPVLATAEVRSELGDSPVFTREDRAR
                     ITRGMMLIGGPGILGRFTALPQIVKTNPLDES"
     gene            complement(34154..34729)
                     /locus_tag="PKM94_04355"
     CDS             complement(34154..34729)
                     /locus_tag="PKM94_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD65904.1"
                     /translation="MHKTVPELVDDFVRGSDAAFTELVHRYQRKVYSLAYQVLGNHLD
                     ADEVVQETFVRIYRRRKELAEVKFFSTFLLRIASNYAIDVLRKQRGHSRMPEDSSSLP
                     GEVQIDLARQVATPSEEFENRALLEEIKRALRELPPRQQLTAILHDIEGYSKAEIAAM
                     LGCPEATVRSNLHIARSKLRKALKKRLRKGS"
     gene            34828..36336
                     /locus_tag="PKM94_04360"
     CDS             34828..36336
                     /locus_tag="PKM94_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD65905.1"
                     /translation="MLAAEVCQSFLRQQLIPVGSSGEHQRIIEAHRHRFFLVGAIGTF
                     MSTLDGSILNVALPTIAADLGADIHLVAWVVLAYTLTIISLMLIFGAWTERKGYDFAY
                     RFAYSFFVLGSAACALSPSIHSLILARVVQGIGSAMFQAVGPGLVTAVFPAEQRGRGI
                     GMMVMMVAAGLMAGPPLGGFILQFLPWQWIFVINLPIGAVGIALAARYFRLLPKPEDD
                     RPIYLAGGAAIATALVAGTLALSMVSEHALRDWQVWAPALAAAGAFLLFLRYESRPAR
                     HMIGLDMFRNRQFSTAIAAAILMFVTMAGGAILLPFYFESVKGYGPSRIGLFLVIIPA
                     IMFVVAPLSGRISDRIGSLFLTTSGAAVAAFGQYLLLHLDAASSDAFIMLVLAVIGLG
                     AAIFNTPNSSSIMGSVGPTERARASSIIGTARNIGNSIGIAMATALFAYFRSRRAVSL
                     GDPAALFVAAFHDVVKVSLGVALLTVPLCLTRHNRPRPGQGGGAPEPPAAGV"
     gene            36413..36488
                     /locus_tag="PKM94_04365"
     tRNA            36413..36488
                     /locus_tag="PKM94_04365"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36446..36448,aa:Ala,seq:ggc)
     gene            complement(36518..>37115)
                     /locus_tag="PKM94_04370"
     CDS             complement(36518..>37115)
                     /locus_tag="PKM94_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014997840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD65906.1"
                     /translation="SETYRSIARKYFSGAIIVADRFHVVRLINQHFLRVWKLHDPEGR
                     KHRGLISLMRRHQWHLSNEQHANLMHYLAAYPVLQQLYVTKQRLVRLMLLKTVTAKRA
                     RKKLPQLTALLEQLLESPLRVLARTLTSWLEPIVAMWRFSKSNGITEGFHNKMEMISR
                     RAYGFRNFENYRLRVLTHCGWDGIINRVRMDVRPPLMG"
BASE COUNT         6339 a        11286 c        13298 g         6192 t
ORIGIN      
        1 gggctacggc gggcagcgct tcgcctacga ccgcacggcc attcacgtca acgagtggga
       61 ggagtactat ttccagccgg tttactaccc cgtgtaccat ccgtggacgc tggcgggcaa
      121 cgtctatatc gactacccgt tcggcgcgac cgtgtacatc aacggcattt actggggctg
      181 cacgccgctg tacctgccgc gggtgtacgt cggatggcac acgatcaccg tcatcgacca
      241 ctgggggtac tgctgggagt cggatatcca tgtgtcgcgc taccacacgc tggtgctcga
      301 ccatcaccat atccgcccgg cgccccacgt gaagtcgaag taccgcgaag tggtggtggc
      361 cggctaccgt gacccggtga agaacggtta cccggattat gtcgcgcgga ccaagcagat
      421 cgcgagcgcc aagggtgtgc gtacagaaga ggtggccgtg cagcggccgt cgggcgaacg
      481 cacagtggag aaggtggcga tcggtccgaa gacgcacgtg cggggggcga ccaatttggt
      541 caagaccgac cgcgggttcg aaacggccgg gctcgtcccg gattgggcgg agtcgtccta
      601 cagccggtct tccaagaagt tcaccgccgg ctccgagggg cgcagcacca agccgtcgga
      661 cggctaccag gggcccggga gcgggtatgc ctcaggcaag cgggacgtgg tagtcggcaa
      721 ggacgggcag acggagagca agaatcaggg cggagccgtc acgcaggaac gcgagcgcac
      781 cagctccgaa tactaccagc ggaagtccgg ttcggatgcg ggccgctccg gctttaagga
      841 aaatgcgcag cggcgcgagg tcgaagtgaa ccagggccga tccaaaggat cgagcgggtc
      901 gcgaccctcg tacaagccgt ccgacggcgg ctcctccaag gggaagggcg caaccgtcgc
      961 cccgccgagc aaaccttcgg gcggatcaag tccctccgga aagagttcgg gcggcagcgg
     1021 gacagtgaag agcgggggcg gcggaggcaa aggaaaaggc ggccggtaaa aggcagactg
     1081 acggagactc cccggggctt ctaccccggg gagtcgaccg gagggagttg agaaaatgat
     1141 caagcggata ttcatttccc tggtgtgcgc cggtctcctc ttctcgctcg gcggaaacgc
     1201 cggggcggtc gagaagaaga gcaaggacga caaggccaag accgagacga aggccggtgc
     1261 ggtgaagaag gacgctcccg tcaaaaaaga tccggcggcg gcgaagaagc cggcgaccaa
     1321 gcagttcgac accttcgtcg atgaaaatgg aaatgggatc gatgaccgcc gggagaacct
     1381 gaagaacaag gcggtggcgg aatcgcaggc cgccagagcg aaggcgcagt cggtctcggc
     1441 caaacaagcg gagagcaaga aagcggacgg ttcgaagaag gcggactcag tcaagaaggt
     1501 cgagccgaag aaggccacgg acaagaagga cgggaagtag taatcgggca gtgaagtgag
     1561 gggaccgaag atggagccgg acgaacagcc cggctttctt ttttgggggc cggttcgggg
     1621 cgagttgaac gtgagaagtt gtgatcgccg gaatcatttg ggcttgactt gcgttataaa
     1681 tcgtatacat ttgttagcgg tctgacaggt atgctgccaa aatggcagga gcccccggtg
     1741 ggtaaacact aagctgttat ggcatttgag aatttaacag agcgcgaaaa gcaggttctg
     1801 tcgaatctga tcgagcacta catccgttcg gcagatccgg tcgggtcgcg ggtgatcgcc
     1861 cacaagttcc ggatgggagt gtcttcggcg acgatccgca acacgcttca ggatcttgag
     1921 gagctggggc tggtgcgtca gccgcacacc tcggccgggc gaatccccac cgacctgggg
     1981 taccgggtgt acgtggacta cctcctgaag ccggaggatc tcacggagtc ggagaaggag
     2041 cggatcaagg atgtgatcgt gagccacgga cgggggttgg atgatatcct ggggcagacc
     2101 tgccgggtgc tgagcgatat caccaatcag ctcggagtgt cgatcgcgcc gcgctttgac
     2161 cggggggtgt tgcgccggat cgagttgatt tcgctgtcgc agggtcggat catggcggtc
     2221 gtggtggtcg aatcggggct ggcccgcagc gtgattgtcg aggtcgaaac gagcatcgac
     2281 gaagcctcgc tccgcgaggt ggagcaggtg ctcaacgagc ggctcgccgg gctgtcgctg
     2341 ggggagattc gcaccacgat cagcaagcgg ctggccgacc acaccgggtc ggggcgcctg
     2401 gtcaagctca tcatcgattc gcgggaccgc atctgggcgg atgatccggc gggggcgatc
     2461 cacctcgccg gagtcgagcg gctgctcagc cagcccgaat tctccgagat cgaccgcatc
     2521 acgcgcattg tccgcctgct cgaggacaac cgtacgctca aggagtttct cagcagcgcc
     2581 tacgaagaag ggctgttcat caccatcggg cgggagaaca agtttgatga gattctgaac
     2641 tgctccctgg tgacgtcgtc atacaaagtc gggagtattg ccgggaccat cggcataatc
     2701 gggccgacgc gcatggagta cgggaagctg gtgtcgatcg tcgactacac ggcgcgcgcg
     2761 atcaccgagg tgctctcggg catgagcagc aaagagggag aatcgaagga tgcgtaagaa
     2821 ggatatcgag ccggaagacc cggtggcgcc gggagggcag ccgtgcgaaa gtgtcgagcg
     2881 cagcggatca ggggaggcgc ctccggcggc cggcgtagaa aacggcgacg ccgaaccggg
     2941 cccgccgcca ggggagatcc cggaagagga gcagctccgg cgacggatcg ccgagctgga
     3001 ggaccgcctg ctgcggacgg cggccgattt cgacaactac cgcaaacggc aggcgcgcca
     3061 gtacgacgaa ctggtgaaga cggccgccga ccgcgtcctg tcggacctcc tcgacatcgt
     3121 ggacaatttc cgccgggcgc tcgacgcggc cgccggcgag gggaacatgg acgcctaccg
     3181 gcagggcacg gaactgatct acggtcagat gaccgagctg ttggcgcgct acgacgtcac
     3241 accgatcgag gcggtggggc ggccgttcga ccccaacgtg cacgaggcgc tcctgcgggt
     3301 gccgtcggcc gagcacccgg cggggaccgt cgcggtggaa gtgagccggg gctaccggcg
     3361 gggggagcgg gtgctgcggt acgcgaaagt gggcgtcagc acgggagcgc cggaggcgcc
     3421 cgacgaaggg aaagagaatt gagtgagata gtgagggaag gagttttggc agcatgggca
     3481 aagtaatcgg catcgacctg ggaacgacca attcggtggt cgcggtcctc gagggccacg
     3541 agccggcagt catccccaac caggaagggt ctcgcatcac cccgtcggtg gtggccttca
     3601 cccgcgacgg cgagcggctg gtgggcgcgc cggcgaaacg ccaggcggtc accaatccgg
     3661 agaacacgat ctcgtcgatc aagcgcttca tgggccgccg gatcagcgag gtgcagtcgg
     3721 aagagaagct ggtgccgtac aagatcgtcg ccagcgccga aggcatggtg caggtggagg
     3781 cagccggcag ccgctacacg ccgccggaga tctcggcgat gatccttcgg tcgctcaagc
     3841 aggcggcgga gaactacctc ggccacgagg tcacgcaggc ggtgatcacc gtgccggcct
     3901 atttcaacga ttcgcagcgg caggcgacca aggatgcggg caggatcgcc gggctggagg
     3961 tcctgcgcat catcaacgag ccgaccgcgg cctcgctcgc ctacgggctc cagaagaaca
     4021 agaaccagaa gattgcggtc tacgacctgg gcggcggcac cttcgacatc tcgatccttg
     4081 agatcggcga cggggtgttc gaggtgcgcg ccaccaacgg cgacacccac ctgggcggcg
     4141 acgatttcga ccaggccgtg atcgactggc tggccgacga gttccgccgg caaaacggaa
     4201 tcgacctgcg caaggaccgc atggctctcc agcggctgaa agaggcggcc gagaaggcga
     4261 agatcgagct ctcgtcgacc atgcagacga acatcaacct gccgttcatc acggccgacc
     4321 agaacggccc gaagcacctc gacctgacgc tgtcgcgggc gaagcttgag cagttgaccg
     4381 accagctcac gcagcggacg gtcgaaccgt gccggcgggc ggtgcaggac gccaagctct
     4441 cgttctcgga catcgacgaa gtgatcctcg tcggggggat gacgcgcatg ccgcgcatcg
     4501 tggcgctcgt gaaggagctt ttcgggcgcg agccgcacaa gggcgtcaac cccgacgaag
     4561 tggtggcgct cggggccgcg atccagggcg gaattctcgg cggcgagatg agcgagaagg
     4621 acatcgtcct gctcgacgtc acgccgctct cgctcggaat cgagaccctc ggcggcgtca
     4681 tgacccggct gatcgagcgg aacacgacga ttccgacgag caaggcgcag atcttctcga
     4741 ccgccgagga tagccagacc gccgtgtcga tccatgtcct gcagggcgag cggcccatgg
     4801 cgcaggacaa ccgcacgctc ggccgattcg acctggtcgg catcccgccg gccccgcgcg
     4861 gaatcccgca gatcgaggta aagtttgaca tcgacgccaa cggcatcgtc cacgtgagcg
     4921 ccaaggaccg ggcgacgggc aaggagcagt cgatccgaat cgaggcctcg tcgggactct
     4981 ccgaacagga gatcgaccgg atggtcaagg atgccgagcg gtttgcggcc gacgacaaac
     5041 ggcgcgagga gctgatcaag gcccgcaacg aggccgacca gctggcctac tcgaccgaga
     5101 agaccctcaa ggaggcgggg gacaagatct ccgccgagga gcggagccgg gtcgagagcg
     5161 cgatcaggaa actgcgcgag gcgattccgc gcgagtcgct ccaggacatc acgagcgcga
     5221 aggacgagct cattgcggcg tcgcacaagc tggccgagga aatgtaccgc caggcgcaga
     5281 gccagggatc gccgggcggc gccccggcgg gcgaggcccc ggcgggcggc gcccccggcg
     5341 gcgagggtgc ggtcgacgcc gactttgagg tcgttgacga cgacaagaag aagtagacgc
     5401 agcccggatg aacaaacgag actactacga ggtgctggcg gtcgaccgcg gcgccaccga
     5461 agagcagatc aagtccgcct atcgcaagct ggcgttgaag taccacccgg accgcaaccc
     5521 cgggaatcag gaggcggagg agaagttcaa ggaggcgacc gaggcgtacg aggtgctgaa
     5581 ggatccgcgg aaacggcaga cctacgatca gttcgggcac gccggggtgg gcgccggcgg
     5641 accggggttc ggcggattcg gcggcggatt cgaggggttc gacctcggcg acgcgctccg
     5701 ggcgttcatg cgcgacttcg gcggcagcgg ctcgatcttc gatgatctct tcggcatggg
     5761 catggggagc gggacgcgcc agcggcgcac cgtgcgcggc gaagatctgc gggtgcggat
     5821 cccgctggca ctggaagaaa tcaacacggg gctggaaaaa tcgatccgcg tcaagcggct
     5881 ggtcacctgc gacgcgtgcg acgggaccgg cgtggcggcc ggatcgtcaa agaaaacctg
     5941 cccgcagtgc aaaggacggg ggcaggtgcg caccgtcacg cgcacgttcc tcggcacggt
     6001 ccagcaggtg tcgacctgca gcatgtgccg ggggtcgggg gagatcatcg ccgatccgtg
     6061 tccgagctgc ggcggcgagg ggcgggtgcg cggcgagagc accgtcaaaa tcaacatccc
     6121 ggcgggcgtg gcgagcggaa actacctcgc ggtcgagggg atggggaacg ccgcgccggc
     6181 cggcggcccg cccggagatc tcatcgccgt gttcgacgag caggagcacg agacgttcga
     6241 gcgccacggc gacaacgtgg tcatggagct gccggtgtcg ttcgcgaccg cggccctcgg
     6301 cggaacggtc gaggtgccga cgcttgacgg gtcggccaag ctgaaagtcc cgccgggcac
     6361 acagccgggg aaagtgctga aactgaaggg gaaggggatc ccccatctgc acaccggcgg
     6421 ccgcggcgac caactcgtgc gcatccatgt ctgggtcccg accaggctcg aggcgcgcga
     6481 gcgggagatg ctcgaacaac tcgcgcggtg tgaatcgttc aaagcgcccc agggcgggaa
     6541 atcgttcctg tcgaaactgc gcgagacact cggagtgtaa cggaggcgcg cctatggcgg
     6601 caacttcgcc ggaatcgatc tacgaaattc gcgtgcgggt cccgcgcgct caggtcgacg
     6661 cattgtcgga tttcatcgtc gaacgaatca gcgccggact ggtgctcgag gaggaggaag
     6721 ggtccccgga aacacgggtg ctgttctacg ccaaccccga ggcggtggcg gcgcagatgg
     6781 cgatggtcag ggggtatgtc gtgcagctgc ttggcgcgga cgtcgacccg gggcggctgg
     6841 tcacggccac cgaaatcagg aaccgggcgt gggaagagga ataccggcgc tcagtgcagc
     6901 cggtggtgat cggcgacatc gtcatccgac cgccctgggc accggcgccg atcgccaccc
     6961 cctacgacat cgtcatcgag ccgaaaatgg cgttcgggac cgggacccat gagacaaccc
     7021 ggtcctgtct gaggatcatc cggcagcatc tccagccggg agggcgtgtc ctcgacctgg
     7081 gctgcgggtc gggaatcctg tcgatcctcg ccgacaaaat gggtgccggc tacatcaagg
     7141 cgattgacta tgacgtgacg gcgatcgaca actgccgcga gaatttccgg cgcaacgcgg
     7201 tggcggcccc gcacgacatt ctcttcggct ccatcgggaa atgccggggc gaccgtccct
     7261 acgacctggc cgtcgccaac atcatcaaga gcacgattct ggagatgctc ccgcggctgg
     7321 tcgagttgac cggccggccg ggacacctgg tgctctccgg gctgctcgaa caggatgttc
     7381 cggaggtggc ggctggcctc gaccgcctgg gcgcggccga ccgggcggtg ctcccggaca
     7441 acgagtggcg cacgatcacc atcacgtttg cctgaaccat gccgaccccg ctccccatct
     7501 tctacgctcc ccctgaggac ctctccggag acgcggttgt gctccggggg gatgaggcgc
     7561 accatgccct gcgcgtcctg cgcctgggca tcggggaccg cctgctggtg atcgacggtc
     7621 tcggcgccgc gcggcggggg gagatcgtcg aggcgggagc gcggggggcg acggtgaccg
     7681 tgcgagtccg cgacacgatc cgcaatttcg gggagccggc cgtgcggctg acgctggcgg
     7741 ccgggctgtc ggcgggggcg aagttcgacg ccgtggtcca gagaggaacc gagctgggcg
     7801 tgcagcggtt cgtgccgatt ctcggggaga aatccaaggt caggatggag gacgccgggc
     7861 gggcggcggc ccgggtgcgc cgcctggaga aggtggccct ggcggccgtc aagcaatgcc
     7921 gccgttccta tcgcccggag attgccctgc cgaccggcct cgagtcgttc ctcgcgtaca
     7981 ccgaccccga ggccctgaac ctcgtgtttc acgccgcgcc ggggggaaag ccgctctcgg
     8041 cccaggcgat cccgccgggc gtgtcaaggg tctcgctgct ggtgggtccg gaggcggggt
     8101 tttccgaggc cgaggcgggg cgggcgatcg cggccggcta cgcgccgatc agtctcggcc
     8161 cgcgcatcct tcggagcgag acagccggtc cggtggtctg cgcgctggtc atgcacgcgc
     8221 tcggcgagtt gagttagcgc ccgatccgcc gatacagaga gtgtatgaat acactcattc
     8281 tcatctggat cggcgcggtc gggttggcga tcggctcgtt tctcaacgtc gtcatctacc
     8341 ggctgccgct gaagaagcgg ttcggcctcg ggcggtcgat ctgtccgaag tgcgggacgc
     8401 agctgcggtg gtaccacaac attccgctgg ccagctgggc cgctctgcgg gggaagtgtg
     8461 cattctgccg gcagccgatt tcgtggcggt acccgctggt ggagctgatg acggccgggg
     8521 cgtacgtgta cttctgggcc tacctcgggt tcagccccat gttctttgtc tgggcggcac
     8581 tcgtctcggc cctcatcgtg atcatcttcg tggatctcga ccaccagatc attcccgatt
     8641 cgatcacgct gtcgggcatg gcggcggggc tggcggcgtc gtttctaccg aacgggctcg
     8701 gcatcgtgca gtcactcatc ggtctcgtcg tgggtggagg atcgctctac ctgatcgcgg
     8761 tgctggggga ggcgctgttc cgcaaagagg cgatgggcgg cggggacatc aagatggcgg
     8821 cgatgctggg ggccttcctc ggatggcaga aagtgctcct ggtgtttctc gcggcggccg
     8881 tgatcggcct ggtcgtgggc gtcatcgcca tgctgatctc atcgaagatg cgccgcgacc
     8941 ggatgattcc cttcgggccg tacctggcgg tggccgccgt gatcagcggc ctctacggcg
     9001 accgcatcat cgggttctac ctgacccgga tcgtcggcct caactgaccc gggtgggggc
     9061 aggcggaagg gatcgaacgc ggtatgaggc ccggccggca caagttccag accgaggtgc
     9121 aactgggcat tctcgccctg gtcttcctgc ttttgtttct gaatttcacg tccaattttc
     9181 tcctttacgc cgcgcgctcg agcaaacggg cggcggtggc ggcgcacctg gccgaggcgg
     9241 ggcaggcggt cctccggctg atggctgagc ggggggccga gggcgcgacc ggggaagggc
     9301 agtcggaaat catgcgccgg ttccgtctcg agagcctgtg gatcctgccg gcggcgcccg
     9361 agagccgggc ggtcgaggac cggcggcgct ggctcgcggc ggtggccgac cggtttcccc
     9421 cggaccgggt gccgggaatt gtgcggcagg tcgtcgccgg aaaaccgggc cggctcaatc
     9481 gcggggaagg cgcggagtat ttccttctcc aggcggcgcc gggacccggg ggggcgaagc
     9541 tgctcctggt ctcccgccgc gagccggaac tggcccgcct cgatgacgtc taccggctgg
     9601 cgctggtcat gtcgctcatt gcgctggtgg ccaccgccgc ggtgtatgtg attctcgcac
     9661 ggctcatctt tgcgcccttc cggaaggcca aggaagaggc gcgcacggcc ggccggacgg
     9721 tggacgagag cgatgacgat gtcgaggtgc tgctcaacga ctaccggcgg atgatcgcgg
     9781 agctgcgcga gaaagagcgg gaactgcttc gcctcaacct cgagatcagc cggcgggccg
     9841 gctcgctcga gcagttcaac cggtacctgc tggcctcgat gccatcgggg attgtcacgg
     9901 tcgaccgggc gggcaggatc cagacgatca atgcggcggc ggagcggatt ctcggcctgc
     9961 gggaaggcga ggcgcgcggg cggttccacg cggagctgat gccccgggaa ctggcagagg
    10021 ctatcgcggg gccgcttgtc gacgaagcgg gcggggagta tcgggaggtg acgccggcgg
    10081 ggctgccgga tacaccggtg ctggggattg cggcctcgac ggtgtgggac ggagaagggg
    10141 agccgatcgg cgcggcgctg ctgatcaatg acgcgaccga gatcactcag ttgcgggaac
    10201 agctcgaacg gcggttgcgg ctggcggcgc tcggcgaaat gtcgggcggg cttgcgcacc
    10261 agttgcgcaa ctcgctgggg gcgatcgccg gctacctgaa ccttctgaag cggcggctgg
    10321 gcaagcgggg ggtggaggaa ccggcggtgg cggagctcgc ggacgaggcg gcgcaggccg
    10381 aatcgctcgt gcggcggttc ctggaattcg cccgcccgct gcaggcggat ctccaggcgg
    10441 tcccgctgcg cgacctgatc ggccaggcgg cggcgagttt tgccacgcgg gaagcgtatc
    10501 cgcgggtcga aattgcggtg ggcgaaatcc ccgaggtcgt gattcccgcc gatccgctcc
    10561 tcttgcgcca ggcgcttctc aacctgatcg agaacgcagc caacgcctac tccggggcgg
    10621 ccgggaaaat cgagatcgac gcggcggcgg ggaacgggga aacggctatc cgggtccgcg
    10681 accgcgcttg cgggatcgcc ccggagaatg tggcgaagat cttcacgccg ttctattcct
    10741 cgcgcccctc gggcaccggg ctggggctgg ccctcgtcag caagattgtc gacctgcaca
    10801 aggggcggat cgaggtggat tcgcgggtgg gccgggggag cacgtttacg atcaccctgc
    10861 cggtcggcgc gccgcagccc gccgccgccg aagggccggc ggagatgcgg gtacgggggt
    10921 aaggaatccg gggcaaccgg tgccgcactg ttttctcacc aggataaagg gagggttggt
    10981 cgcgcctggg atgtgggcgc cgaccggggt ctccggccgc ggcaaacccc gccgggccgc
    11041 cacggcggcc ggggaaaaaa ggtcttttgc ggatccgact ttttgttata tttggggccg
    11101 attctctagg aggaattgat gtattcaccg tcggatttca gaaaagggct gcgcataata
    11161 gtcgacgaac aaccgttcta cattgtcgat taccagcatg tgaaaatggg ccgcgggaga
    11221 gccaacatcc ggacgcggct gaaacacatg aaaaccgggg cggtgatcga aagggtgttc
    11281 ggctcgaacg acacgttcaa ggcgcccgac acccaggaac gaaaaatgca gtacctgtac
    11341 gagaacgccg gggagttcag tttcatggac accgagtcgt tcgagcaggt ggtgatcctg
    11401 gctgattcgc tgggcgacgc caagtggtac ctggtggaga acagcgagta ccgggtgttc
    11461 ttcctggatg gtcagccgat ctcggtcgat ctgccggcat cggtcactct cgaggtcatc
    11521 tatacggaac cggccgcccg cggcgacacg gtgagcaatg tcaccaagcc ggccaagctg
    11581 tccaccgggc tcgaggtgaa agtcccgccg ttcgtcaagg agggggacat ggtgaaggtg
    11641 gacacgcgga cgggcgagta tctcgagcgg gcgaactagt ggcggcgggg gaccgccgcg
    11701 tcatcggggt cgacgaatcc ggcaaggggg acttcttcgg cccgctggtg gtcgccgcgc
    11761 tggcggcgcc cgacagcgcg ctggcggacc tgaaggggct gggggtgcgc gacggcaagg
    11821 gaattgccga ccggcgtctc ctgacgatcg atgagcagtt gcggcgcgcc taccccttcg
    11881 cagttgaaat catgccgccc gaggaataca accgggctta cgagaaaatc cgcaatctca
    11941 atcggctgct ggccgatctc cacgcccggg cgatcgaccg tctgctgcgc gaccacccgg
    12001 cggatttggt ggtggtcgac cagttcggca agactgccct cgtggccgag gccgtggcgt
    12061 tccatgggca ctcggtcgcg ctcgagcagc ggtttcgcgc cgaggaaatt ccacaggtcg
    12121 ccgccgcttc catcatcgcc cgggccgaat tcatccggca actgcagacg ctctcgcgcg
    12181 aaatgggagt ggaattgccg aaaggggcgg cggcaatcgt ggatcgggcg ggagccgcgc
    12241 tggtgcggca gcacgggcgg gacatactgc ggaagacggc caagatccat ttcaagaact
    12301 atcagcgagt catcaatcct tctttgttcc cgtcgtgagg ccttttcgtg gccacaaggc
    12361 tttgtgtttc agggggatac ggaactgaga cggcgaggcc tccggtgccg gtgacaagtt
    12421 ccgacgatgc ataaaccgtc ccccggttct gccgataatc tgaaaaaaga taatcagtta
    12481 gcccgaccgg agggtgtatg gctttgccgt gggcgccgtt tggcgccgcc ttgttcatgt
    12541 tggcgctgct ggtgctgctc agactatatg gcaaatacct ggcggatcaa taccgcgagc
    12601 agttccgcaa tctcgtcgtc ggcgtcggcc tgttcggttt ggtggccgtg ctctacctcg
    12661 gcgaggcgac gggggcgacg gccgctgtgc cgttcatttc gcagccgccg ttcctggagg
    12721 taagcgcggc gattctcgcg atcaccgggg ccgtgctgac ggcctccggg gcctccgtgt
    12781 ggctgccggc gcgtcggacg gggaagatcc tccatgacgt cccgtcagaa ccgcttcggc
    12841 gcgaggcgga aatcgtccgc gcccagctcc aggacgcact cgccctcggg gcgccgctgg
    12901 cggattcgtt cacggcgatt gcgcgtgtgt tgaaagcggc gctcggggga gaattgagtt
    12961 cgctggcgat ggcatacggg gagtgggata tccgccgcta tacggccggc ggcaacggga
    13021 cgcatttgac ggagaagtcg ctggcgcggg aggcggcggg cctcccgcag gagaatgaca
    13081 gcccgcctcc ggaccgaccg ttcgtgaccg tggatttcga gcgtttcctc gagccggcgc
    13141 tggccgcggt gcttcgccgc cagcaactgg cggcggcgct gttggtgccg ctcggcgatc
    13201 cgggcggcgc ggcgatgctg gtcgcggcgg cgcgctcggc cgaggaattc ggcgagcgcg
    13261 aacgctacct cgccgcgcag gtgcgctttc cgctgcggct ggcggtggcg gtcgaacggg
    13321 aacgcgaccg gctggcgcgc gggatgaagc ggtcggaggc gctcgcccgt ttgtccgccc
    13381 aggcggcggc ccggctgggt gacatcgacc ggctgttcgc cgaagcagcc gacgtccttc
    13441 gccggtcgac cggagcggct gcggtgcgga tcgcgctgtt tgaagacgac gggcagttcc
    13501 tgcggtcgcg ggccgcggcg gcggcgcacc cggtggagaa cgccgtgccg gcgaccgggt
    13561 acctgatccg ctcgctcatg cccctgcacc agtcggtcca ggcgacgggc gaggcgctcc
    13621 tgatcagcca ggccgaggcg gccgagcgga tgtcggcggc ggaggcgcaa caaatatacc
    13681 agagcgatct ctcgaccgcg ctcctgtcgc ccatcaccgc gggaaccgag acgctgggga
    13741 tcattgccct ggccgaccgc cgggggagcc gccggttcgg attcaccgac gacgaccgga
    13801 cattcgccgc ggccgtggcg gcggtgctgg cccaggccgt gcgcctgcac gagacaccct
    13861 ggggcgcgct cacctggcgg gatcgcgaga gtggcgagga tgcggaccgc cgggcggagc
    13921 tgcgcggccg gattcgctcg tcgctgaccg gcatcctcgg gtcggtggag atgctccgcg
    13981 cccgcccggt cggccctgag gccgaacgct tcgtggagat catcgatcgg tcggcccggc
    14041 gcatcgaaac aattttctcg gaggagacgc cggcgcgctg atcgccgccg atcggcgaaa
    14101 tcccccggtc acgcagcatg gatgtgccga cctacaagcg gtgcgccgca cgcggcccgg
    14161 aggcggcccc gccggagctg cccgccgcag ccgcgctcta caccggcgga ggcattcccc
    14221 agttgttccg gcgcctcggc accgccgaag gcgcggatgc atggctctat gtgccggccg
    14281 gcactctgga cagcctcgtg ccgacgacgc cggcgacgga gggtcagttc gcctcggcgg
    14341 aagggctgac ggtggacgat agcggtcgct tctgtcctct ggtggtcggc ggggcggtgc
    14401 gcggtttcct cagcggcatg gaccggccgg acggcgctgc gctcaaggcc gcggtgctcg
    14461 actggctgcg gacgcgggag atcgcgactc aggcgacgct gcttccggcg caggcggaac
    14521 tggcggcggc gctggtggcc tgcgacctgt cgccggagcg gctgctgcgc aacgccctga
    14581 acctcctcgt cgagcaggtc ccatcgggac tggcggccgt gtacggcgaa tcggagggcg
    14641 tgtacgggct gcggacggcg gtcggcgaca tcgccatgtg ggacatgctc gaggggacgc
    14701 tgccgcacgc gaccgtgggg cgctggatgg aggggctggc cgtggggcgc cactttctgc
    14761 cggcggggtt tctccccgaa cgccccatgc ttctcgatgc cgcccccgat ttcctgttcg
    14821 tgcatgaggg gctgcgctgc agtcgtcccc ggtccttcgt catcatgccc gtgcccggcg
    14881 acatcgcgcg cgaggccggg gagcggctgc tcacgatcgc gcgcttcatc gccgcgctcg
    14941 atgcggacgg cctgagcatc accgaggatc tgatcaacgt gtgcggccag ttccgtttcg
    15001 cctgcgacca ggggcggccc ggccgggagc tgctggagaa cctcttcgag gtgctgcagc
    15061 gccggatggg gctctcccga atcgtgttgg cggccgccga cggcgggggc gcggccgcgg
    15121 tcggccgccc cgagatgccg ccgtcggtgc gcgccgaatc ctcaccgggg atcgccgcgg
    15181 cgattcaggc ggcggcgcgc ggcggccgca gtttctcgcc cgatgtgtcg gccctggggc
    15241 tcctcgacga cgacgaggcg aagcgctact acctggacaa cgtgcgctcc gaatactgcg
    15301 tgcggccgcg ggatgccggt tgcggcgaaa cgctcgtcgc cttcggccat ccggagaccg
    15361 ggctccgact gcggcagttc gagcgcctcc tcgactcgac cgccatgctg ctggacctct
    15421 attatcagtt ggaggggaga ctcaacgcgt caccgggagc ggcggatgcc cagggaaaac
    15481 cggagcggcg ctgggcgaca gcctcggcga tggcggaggg acatttccac gccgtgtacg
    15541 acaccctctc agggctgctg gggaagacgg aggtggcggc ggcgatcggc ggcgcggccg
    15601 ctcagactgg acattctccg gccggcgagg cgctcgcccg ggtcgtgcgc agcctcgacc
    15661 acctgcgcgc cctcgtgcgc gacacggcgt cggcggcgga tgagccggtc gcggtcgagc
    15721 gggcgctggc cgaactgccg ggcatgctgg aagggaaggc gcaccacttg gaggacctaa
    15781 ggggcctcgc gctgacagtg gcaatcgggc cgccgtcggg ccgctcgttt tcgattctgc
    15841 gccgcgatct ctacgaccat ctctaccctg cgctgctggc ggtcatggag gcggcggttc
    15901 ggtcgggcga agtgcgcatc cgggcggcca ctcaccgggg agccgacgcg gtggaagtga
    15961 cctgtcccgc gccgctgctc ggccggagaa gtatcgcgga cgttatgcag gtatggaccg
    16021 gggaggcggg agaacgcgag ggaggcgggc gcaccctgtt cgacgcgggt cggtacgtat
    16081 gggtgtgcga cgcgcgcggg cgcgatcccc agcgggcgtg gctggcgatc cggcacgaca
    16141 tcaacaccct ccggcccgac cggccggtgg atcggcatga ccgggaggtg gggtgatgcg
    16201 cacttcgggg atactcattc agaaatatta ccacgtgcgc acgggccata agctggccgg
    16261 catcatcgcc caggtcgaca cgctctcgct gatggcggcg cggcaactcg gctgcgactt
    16321 ctgcgccgtg ttttaccgga ccgcgggcaa ccgcacgctc attcccgtgt cgtatcagca
    16381 cgggagccac ctcagcattc ggaacctcga gttgctcgag aaggcgtggg gggaggccga
    16441 ggtggagaag acgccgccgg agcggggttt gcgggacttg ccggccaggg cgtctgcggc
    16501 ggacgctttc ggggccgcga acggcttcgc ctgccgctac cttctcccgg tgcacgagga
    16561 tgggcggctg tgcgcggtgg tgatcggcta ctggtttgag cggccgggcg cgatcgccgg
    16621 cgaagcggag cggatgctcg atctggtcgg gtcggtgctg cgggcggccc tgtcgatcgc
    16681 cgaccaactg ctcgtggtcg agaactactc gacccgcctc tccgagctgc tcccggtgtt
    16741 cgaggcgccg ctggaggaaa cgaagttctc ggccctggtg agcgcgacgc tcgggcgggc
    16801 ggcgcggatc tgcccgacgg cgtacgtggt gcttctggcg cgcgaccgca ggaccgggag
    16861 aatcggctgc gcggagtcgt acggcgaccg gaaaccctcg tccgaactgt gcgactttat
    16921 cctccgggcg gccgagccgc tcacgcaggt ggaggcggcg ggcgatgagg atgacacgcg
    16981 ccggttccgc tgccgcgatc tcgcgcgggc cgataccctc ggcctgaccg acctggtcgt
    17041 gctaccggtc gcgcccggcg acgagttgta ctacctgctt gcggtcggcg cgacggggga
    17101 gcacggcctg tcggcgaacg acagggagct gctgtcggtg tttgcggtgt ttgcccagac
    17161 ggtgctccgg aacgcgctcc tcttccggcg gctgcagcgg gccaaccggc tcctcgaacg
    17221 gtcctccgac cggctcgccg acgccgaggc gatggcggcg ctgaccgatc tgacctccgg
    17281 cgtggcccac gatttcaaca acatcttcgg cgggatcatg ggccgcgtgc aactgctccg
    17341 cctccgtgcg agggaactcc cgcagttcaa cgacgagctg gcgaaggtcg aacggctggt
    17401 cgccgagggg gcggagacgg ttcgccgtct gcaggagttc gccaccagcg ggcgggccaa
    17461 ggatgtgcag ccggtcgacc ttgtcgcgct cgtgcaaaag acgctgcagc cagcccaggc
    17521 gcgctggcgg gaactggcgg tcagcaagag tatccggata gaattccgcc ccgaaacgga
    17581 ggccgcggtc atcgagggtt gcgctgagga tctcgccacc gtcctgcgaa aacttctcga
    17641 aaacgccgtg gagcacgcgc cggaaggatc caccgtggcg gtggcgctgg cggccgacgc
    17701 ctccgactac cggctgcagg tgtgcgacag gggcaagggg attccgccgg aggcaggcaa
    17761 gaagatcttc tacccgttct tcaccacaaa aacggagcgc ggggcggggc tgggcctggc
    17821 cattgtccac ggcatcgtgt cgcgacacgg cggcaaagtc ggatacgaat gtacgccgga
    17881 tgccgggacg gtgttcaccg cggcgttcaa gcggcccgcg acggtggccg agatctccga
    17941 ggtcaccacg cggcggaagg cgagcgccgc cgggctgcgc atcctggtgg tcgacgacga
    18001 cgaccagatc cgggaggttc tccgcgacat gctcgtgatc gccggccacc aggcggtggc
    18061 gtgcgcgaac ggtttcgagg cgctggagac cgtgaaccgg gagccgtttg atatcatgat
    18121 caccgatctg gggatgccgg ggatgtccgg gctcgatctg gccggggctg tgcacgagca
    18181 ggcgccgaaa atgccgatcg ccatgatcac gggttggggg acgcagttgg accagcggga
    18241 gatagcggcc aagggagtgc gggcggtgct gtctaagccg tttcatctca aggatattcg
    18301 cgcgctcgtc gacgaactgg cgggcgcctg agaactttct cacagccagg acaaaaccgt
    18361 tgccttttgc gcgccgcgcc gtatattatt cccggtaaca gtcttcgggg cgaggcgaaa
    18421 atcctcgacc ggcggtgaga gcccgcgact cccacgagag tgggactgag cctgtgaaat
    18481 tcaggcgctg acggtacagt ccggatggga gaagattgcg attcgaccgg cggtgcggcc
    18541 gtgtatgccg cccggcggcc cgtgcgcctc ggccccgaaa ctctgcattc ggggtttttt
    18601 cgtggccgac gagacggaca tcaggtacat gcaacgggcg ctcgagctgg cggcgcgggg
    18661 acggttccga accgcgccga acctgatggt cggggcggtg attgtccgcc aggggcgcat
    18721 catcggcgag gggtatcacc ggaaggcagg ctcggatcac gccgaaatcg cggccatccg
    18781 gaacgccgtc gagccggtgg ccgggagcac ggtgtacgtg acgctggagc cgtgctgcca
    18841 caccgggcgc accggtccct gcacggcggc cctcatcgag cacggcgtgg cgcgcgtcgt
    18901 gttcgccgcc accgatcccg acccccgcgt gcggggaaag ggggcgcggc ggctccggca
    18961 ggcgggtatc cgggtagagg ccgggatcct gcgcgagcag gcggttcggc tcaacgaagt
    19021 attcttcgcc ttccatcgga ccggccggcc gttcattatc ctcaagacgg cgcagacact
    19081 cgatggccgg attggggcgg gcaacggcga ctcgaaatgg atttccagtc cggcctcgcg
    19141 gcggatggcc cacgaactgc gggccgaagc ggatgcgatc gtagtggggg gcggaaccgt
    19201 gcggcgcgac aaccccgccc tgaccgtgcg ccacgtcacc ggacccgatc cctaccgcgt
    19261 catcctcagc gcctccctgc gtttccccaa atcctgccgc cttctggacg acaacgccga
    19321 cgcccgcacg atcatcgcga cgatggccga aaatgtcgag cggtttgcgc ggacccggcg
    19381 cggacgctcg ctcaccttct ggtcgattcg gaggcgccgg gatgggcagc tcgacgtggc
    19441 cgacctggtg cgcaaggcgg gccaattcgg tctgcgctcg ctgttggtcg agggggggag
    19501 ccgggtggca acgtcgtttc tcaaagccgg gctggtcgac aagtatgtcg cgttcgtggc
    19561 ccccaagctc ctcggcacag gaatcgacgc cgtcggcgat ctcggcatcg cctcgatcga
    19621 cagggcgctg accttcgcgc gcactgaaat actcacctgc ggcccggaca tcgtcgtcat
    19681 cgggtatccg aagaggagga agtgagatgt ttaccgggtt ggtcgaagac gtggggacga
    19741 tcgccgacat cggcctgcgg gggaactacc gggtgttgtc gatagtctcg gtgctggccg
    19801 atgagcatct gcggatcggc gattcggtga actgcgacgg cgcctgcctg accgtggtcc
    19861 gggtcggggc ggggacgttc acggtggagg cgtcgcccga gacggcggcg cggacaattt
    19921 tgaatgacta ccggcgcggc gaccggctca atctcgaacg ggctctgcgg gcgggcgacc
    19981 ggctcggcgg gcatttcgtc ctcggacatg tcgacacgac cgggacaatc gagcacgccc
    20041 gatcggcggg ggaatcgcgg gagatcgccg tgaccttcga cccggccttc gatgcgctcg
    20101 tggtcgagaa agggtcgatt gcgcttgacg ggatttcgct gacggtgaat gccgtgcgat
    20161 cggcctggct ggcggtatct ctgattccgc acagccttgc ccggacgacc gcgggcggat
    20221 atcaggcggg ccggcgggtg aacattgaat tcgatatgat tggcaaatat atcattaaga
    20281 tacggcaatc aaccggaagt aatactttaa ccttggataa gctcaggcag agcggatggt
    20341 aagccatggc ggacgagcag atattcaaca cggtggaaga agccatccag gagattcacg
    20401 cggggcggtg tgtcatagtc gccgacgatg aggaccgcga gaacgaaggg gacctgatca
    20461 tggcggccga gaaagtgacg ccggagtggg tcaacttctt cgccaaacac ggccggggtc
    20521 tgatttgtgt ctgcatgccc gaggagcgga tcgcccggct cgcgctgcac ccgatggtgg
    20581 atcggaacac ggcgctccac ggcacgcggt tcacggtgtc ggtggacgcg gtggagggga
    20641 cgacgaccgg gatcagcgcg gccgaccggg cgaaaacggt gcgggtgctg gccaatccag
    20701 cctcgcggcc ggaggatctg gcgcggccgg gacacatctt tcccatccag gcggtgaccg
    20761 gaggcgtcct gaagcgcgcc gggcacaccg aaaccgcggt ggatctggcg cggctggcgg
    20821 gtctgaaccc ggtggggatg ctgtgcgaga ttatggatga cgacgggagc atggcgcggg
    20881 taccccggct ggcgcagttc gccagggagc acgggctggc gttcattacc gtgcgcgacc
    20941 tcatcgccta ccgccgccgg accgaaaaga acgtcgagcg cgtggagacc gtcgacttcc
    21001 ccaccgaata cggggatttc cgccttcacc tgtacaagtc gaaactcgat gaccactacc
    21061 acgtcgccct ggtgaaaggg gaagtggcgg ggaaaaaaga cgtgctcgtc cgggtccact
    21121 cgagctgcgt gaccggggac attttcgggt cctgccggtg cgactgcggg cagcagctcc
    21181 acgcggcgat gcgccaggtc gaccgggaag gctgcggcgt cgtgctctac atgacgcagg
    21241 aggggcgcgg gatcgggttc gccaacaaga tccgcgccta ccggcttcag gaggacgggc
    21301 aggatacggt ggaggcgaac gaatcgctcg ggttcaagcc ggatctccgg gagtacggcg
    21361 tcggggcgca gattctggtt gatctggggc tgagttcgat ccgactattg acgaacaatc
    21421 cgaagaaggt gatcgggttg gaagggtacg gtctggagat caccgagcgc gtgccgctgg
    21481 agttcgcgcc cggaaaatac aaccgccgct atctggaagc gaagcgggac aagctgggac
    21541 actttctctc actgaaacgg atgggagcgt agcgtatggc ctacaagacc tatgagggca
    21601 agctggaggc ggcgggcttt cggttcgggc tggtggtgag ccggttcaac aatttcctga
    21661 ccgacaaact gctcgagggc gcccttgatt gcctgatgcg gcacggggcg agcgaggata
    21721 aaatcgccgt cgcctactgc ccgggcggat tcgaggtgcc gcccgtcgcg gcacgcatgg
    21781 tgcggtcggg gaagtacgac gccgtggtct gcctgggcgc ggtgatccgc ggcgacacgc
    21841 cgcacttcga gtacatcgcc gccgagtcgg ccaagggaat tgcccggctg gcgatggagg
    21901 cggggatccc ggtgatctat ggcgtggtga cggcggacac gctcgagcag gcgatcgaac
    21961 gctcggggac caaggcgggg aacaaggggt ggcacgcggc cgaggcggcg ctcgaaatgg
    22021 tgaatctcta cagggcgatc gaatgaagcg ggcggcggcg gagaacgccg caggcgacgg
    22081 gggggccgcg tgagaccgtt cgaacaaccc cgccggcagg cgcgcgaact ggtgctccag
    22141 gcgctctacg ccaccgagtt cggcagcgcg cggccggagg aagcgttggc cgacctgctc
    22201 gccgaccagc cgctggccga aggggcgacg gcctttgcgc agcgtctgtt cgcctcgacg
    22261 cggcgccggg tcgaatgggc caacgccgcc atcgccgagc tggcggtcaa ctggaatatc
    22321 aaccgcatcg cggccgtcga ccgggtgatc ctgcgaatgg cgctggtgga gctggatgag
    22381 ttccccgata cgccggtgaa ggtggtgctc aacgaggcaa tcgaactggc gaagaagttc
    22441 tcgaccgccg aatcgtcggc cttcgtcaac gggatcctcg atgcgtgggt gaagcggaac
    22501 gaccggggaa cgggcccggg gacgtagggc gagcggccgg cggcgaagtc tgtcccttca
    22561 cttgagaatc agcaattgga tatcggcgtc ggcggccggt gcgacgctga tcgcgcgggg
    22621 cgccgcaccc tcctcgaaaa tcgtgaccgc cacggcggcg gcgtgcttgg catcggagta
    22681 acgcgccgtg atcgccgccg cggtgcggag tatctcctcc gaccggtccc ggccggccag
    22741 gagggtcacg ggggagttgg tgtcgagggc ctcgaggtgg acgtgctggg gccgcttgag
    22801 cgcgagcaga tgccggttgt cctcctcgtc gcggccgacc acgattttgg cgtcgggcgc
    22861 gaggcggaag tggcggccgt acttgagcag gttcagctcg tcgaaagtca gccggtcggt
    22921 gtgcgccatg aggtcgcgca ggcgatttga atagccgacg tcggtgagca gacagccggc
    22981 cgcgggcgtg gggtaatcct ccagaccgaa tgcggcggcc agctcgagct ggcgtttgcg
    23041 ggaacgcccc tcgatcccct cgagcaggtt gcggtcgacc aggccggtgc gttcgggaat
    23101 ggtgggggga agcagcttgg ccgagagcgg tcgcaggagg cggccggcca ggccggtctc
    23161 ttcctcgatg aggcgcagtt tcggcttgat ctgcgacatg ggccgctggc ccaccacctc
    23221 gcccgtgatg atgaagtcgg cgtttaccat ctccatgaag gtccggcctt cggtgagcat
    23281 gaggatgcgg caatcggtgc aggggttcat gtttttgccg cggccgaacc gtgggttctc
    23341 gacgatgtcg atgaatttct gcccgaggtg catgagcttg acgttgaaac cgaacttctc
    23401 ggcggtcggg tacgggttcg agccgcagga ggaccggtcg ccgagatcac agccgaagtg
    23461 ggtcatgaag gtgagggcgg tgacttcgat gttctggcgc aggatgagga gaatcgccag
    23521 ggccgaatcc aggccgccgg agaagagggc gatggcgtgg ccgcgtttgg cgccgctacc
    23581 gttatacatg ggctggcata gtccttcttt acgtcgttgc tgctggcctc tatacgccgg
    23641 ccggcgacgg actgttggcc gcccggacag gcggtgacaa tatacgtccg gccgccgacc
    23701 gggcaagggc aaagaccttg acttgatgct gcggggcggc taatttgtgg cgcaaccgac
    23761 ggtgggaaag gaaagcagcc attctccgcg cgatcaacag ctggctcaag cacttcgctt
    23821 tcgagctcca acggatgttc ttcttctcgc tccgcacgtc ggcggccctc ttccagcgcc
    23881 ctttctacgg gaccgagact atcgagcaaa tgtacaacgt cggggtgggg tcgctctttc
    23941 tcgtgatcct gaccgggatg ttcgccgggc aggggctggc ttacgctttc gcggttgaac
    24001 tggcggaact gggggccaag aactacctgg gtcggatcat ggcgatcgcg gtggtgcggg
    24061 agctggggcc gctcctggcg gccttgatga tcgccgcccg ggtgtcgtcc gggatcacgg
    24121 ccgagctggg ggcgatgaaa tcatcgcacc agatcgatgc gatggtggcc ttcggggtcg
    24181 acccgcgcaa gaagctcatc gtcccccgca tcatcgcgct cctcgtgatg gtgccggtgc
    24241 tgacggtggt gaccgatgtg ctggccattc tcggagggta catcgtcggc acgctcgtgg
    24301 cgcacctggc gccgtcgatc tactgggcgg cggtgaaaga gcgcctcgta ttcggaaacc
    24361 tgttcatcgg tttgctgaag ccggtggtgt tctcggtggt cattgccctg gtgtcgacgt
    24421 acaaagggtt caccgcggag gggggcacca agggcgtggg ccgggcgacc acggaatcgg
    24481 tgatgatttc gtcgatcaca atcctggtgg tgaatttctt catcacccgg gtggtgttcg
    24541 caaccattaa ggggtggctg tgatcgcgct ccgcagcgtc catttcgcct acgccgaccg
    24601 gcccattctc cgggatgtgt cgttttcgct ggcggagggg gagtccatgg tgatcctggg
    24661 gccgtcgggc tccggcaagt cgaccatcct ccggctcatc ctcgggctgg agtgtcccca
    24721 gcggggcgac gtgatcatcg acggcgagag catttgccgc atgaaggagt cacggaagat
    24781 cgatgtgcgc aagaagatcg ggatggtgtt ccaggcgggg gcgctgtttg attcgctcac
    24841 cgtgggcgaa aatgtcgcct actacctgct ggagcacacc cggctgccgt gggacgaggt
    24901 cgaccgccgg gcgcgcgaga tgctcgggtt cgtcgggctc gacgcggacg agatcatcga
    24961 caagctgccc gaggagctct ccggcggcat gcagcggcgg gtggcgatcg ggcgggcgct
    25021 gctgtcgacc aatccccgaa tcatgctcta cgacgagccg acaacgggtc tcgatccgcg
    25081 ttccaccgag caggtgctca agctcatcca gcggctgaac gcggacaagg gaacggcgtc
    25141 ggtggtggtg acccacgaga tcgccgatgc gctcgcggtg gccgaccggt ttgtgctcat
    25201 atcggccggc gaggtggcgt tcgacgggac cttcgaggct ctccgcgata gcgccgatcc
    25261 ccgggtgctc gatttcctcg cccccttccg ggaatcgttt gcggaagtca gcagaaaacg
    25321 ttttatatag agtcgaagcg gcggcggttt ggccgccaaa ggaaaaaggc aagcgacagg
    25381 cgcgcatatg agaagtacgg tcggtgtcaa gtggggcggg ttgaaggtgg ggatcctcat
    25441 catgctggcg atcctggttc tgctctgggc ctcgctcagc ggcggcggaa cctcgatctt
    25501 ctccgccaaa ttcacctacc actcttactt taagaatgtc agcggaatgg tcgccggggc
    25561 gccggtgtgg atggccgggg tcgaagtcgg gaacgtccgc cgcatcgaat tcgtcaacct
    25621 cgacagcctc cggcgggtcc tgatcacctt cacggtcaaa gagtcggtgc acaagatggt
    25681 gacaagcgac gcccgggtgc agctcgggac gatcgggttt ctcggcgaca agtacctcga
    25741 agtgatcccc gggggaatcg gcgggccggt ccttcccgag ggatcgctca ttccgcccca
    25801 agacgtgggg agcgccgaag cggtattcgc ggcggccgag aatgccatcg gtgaggcgga
    25861 ggcgctggcg gccaacctcg atacgctgct cgcccgggcc aacgccggcg tcggcacgct
    25921 cggaatgctc gccaccaacc aggagttgta cgtgcagatg acgcggctgg cctccgagat
    25981 ggccgacctc gcccgcggtc tccaggccaa ccaaaaggaa ctgctgacct cggtgacccg
    26041 ggcggccaac acgatggccg acgtggggga gaagctcgat gactcgacgg ggacagtcgg
    26101 gcggctggtg accgacacca tgctctacgc caatctgact gcgcttacct cgcagttgga
    26161 ctcgaccctg acgaagatca accgggccga agggagtctc gggctgctgg tcaacgatac
    26221 gaccctgtac gtggagatcg ccgctctgtt ggcccggagc aaccggctgg tcaaggatat
    26281 tcaggacgat ccgaaaaagt atctggggtt ttcgattttc tgatgggagt tggaatgcgc
    26341 cgctgccgta tcctcgttgt gcttgccgcc ctgtcggtcc tcgctgtcgg cggttgttcg
    26401 aagtccgagc agcctgcccc gaacccggac cgtccccagg tcgttcccga ccgggtcatt
    26461 gcggtcccgg agggtttcgg cgtgattcgc ctgatcaccg gcccctattc gcccgagatt
    26521 gtctcaaggg gtttctacca cctgcgcggg ccgattcccc tccagccctt cgaactcggc
    26581 ctgcaccagt acgcgctcat ggattccatc gccaactacg aggtgctccg gtaccgcgag
    26641 tttgacggat tcgagagcgt gctcgacagc gtcccgccgg ggaggtacac gctgctcgtc
    26701 tacgtgtcgc ggctgggtcg ggcggagacc aagctggata ctgtggtgag tccggatggg
    26761 gacacgacca tcaccgaccc ggtcaagtcg atgccgccct atgactacta tttccggccc
    26821 gttctcgtgg acagcatcgt ggtggcggac gggcaggtga gcacgatcga tctgtcgctg
    26881 agtttggaga agtgggggcg tccggtccag acggacgccg ggtgggaggc gccgcgggcg
    26941 cgctacctcc tgtacagccc ccggtaaaga ggggatctgg gccggcgacc cacatccggc
    27001 ggcggacgaa cgcgccgctg cgggaatgca ttttgcgctt gcccggcggg caggcgtatc
    27061 gtagattatt ttgatccata cattgcataa tgcgtcggac gaaaggagcg tgattacgat
    27121 gccgcgctat ctcatcgaag tgccccacga ggaagacacg gtcgcctgcg cccgggtggt
    27181 gaaggtgttc ctggagaccg gatcgcattt tctcacccac gcggactggg ggtgcgagga
    27241 cggcgagcac aaggcctgga tcacggtcga gacggacacg cgggaggccg cccgcagcgt
    27301 cgtgccgccg gcctttcgct cccaggcgaa agtgacgcag ttgacctact tcacgatgga
    27361 ggaaatcgac gcgattctcg agcgccacgg ccggggggcg cgggacggct ccggcaactg
    27421 agtccgggcc gggcggggcc ggctagacgg gggcggtggc gagcatgtac tccaggaggc
    27481 ggagggcgcc cgccttgccg ccggccccga tttccatgga cggtccgaca caggcgggac
    27541 agccggcctc acagccgcag ccgcggaggc ggtggtagac cgcctcgcag agccgttcgt
    27601 gctcggtgaa gagcttctcg gccagcccca cgccgccggg gatgttttcg tagaggaaga
    27661 tggtggggca ctcggtgaag ggggcgcgga cctgggagac ggcgcgcaga tcgcgcgcgt
    27721 cgcacatcac ccacaggggg gcgatcttgc cgaggatgtt ggcgaggccg cgcaacgccc
    27781 cgccgaaatc ctcgccgccc accccgaccc ggaaagccac gtcgcccgcg aacgagtacc
    27841 acactgcgtg ggtgtgcatc tcgatctccg gcatggtgag tttgccccac ccgacgttct
    27901 cgtgggtctc gaacttgatt ttcttgaaca gcacggtgac actggtcacc gtgacctcgc
    27961 cgcacgaaat gtcggcctcg ccgaaccgcc gggtccggtc gacatcgagc accttcaggt
    28021 cagtcttggt ttcggcgtcg gtgtagtaat cgacggagac ctctttcacg tacgccttgc
    28081 gcccctccca gtccaggcgg gtgacctggt actggttggc gcggtggagg tagattgcct
    28141 gcgggtggag gaagatgggc gcggagaaga aatcgacctc gccgatcacc tcggagcggt
    28201 tgctctcgtt gaggatgacg aagttttccg gggaggcgga gcgcagcgac acctcctggg
    28261 cggggtagat ttccgacgac cagtggtagc gggcgccggc gtggcggacg atcttggcgt
    28321 cctcgaggta ctcgaggatc tcgcgggtgc cgtcgtcgcg ggcgtactcg tgctcctcga
    28381 aggggagttc gaaggccgcg cacttgaggt ggttgctgcg gatgatgagg ttgttcgggt
    28441 cgatgatgcc ggattcgggg gtgcggccga agatgtactc cggttcggcg cagaggaact
    28501 ggttgatggc ggtcgagttg gcgatcatga cggtaagcga tgtcttcgag cgccgtccgg
    28561 cccgcccggc ctgctgccag agcgaggcca tggtgccggg gtagccgacg atgatggaga
    28621 cgtcgagact gccgatatcg agcccgagtt ccagggcatt ggtggagacg acgccgtgga
    28681 tgcgccccgc ccggagaccc tgttcgatgg cgcggcgctc gttcggcagg tagccgccgc
    28741 ggtagccggc aatgacgacg ccgcggccaa aatccccctg cagctcgcgc tggaggtagg
    28801 tgaggagcac ctcgacgtag agccggtagt gggcgaagat gatggtctgg atgcgctgcc
    28861 gcagaaagag ggcgcccagg cgggcggcct cgttgagggc agagcggcga atcccgagct
    28921 gcttgttcac caccggcgga ttgtagatga gaaagtgctt ctcgccggcg ggcgcaccgt
    28981 tgttgtccac cagctcgacc gggcggccga tgatccgcgc ggtcaactcg gccgggttgg
    29041 cgatggtcgc cgagcagcag ataaactgcg ggtcggagcc gtaaaaccgg cagatccgcc
    29101 gcaggcgcgt gatcacgttc gccaggtggg agccgaagat gccccggtac tggtggacct
    29161 cgtcgatcac cacgtacttc aggttctcga acagcttgat ccacttcgtg tggtggggga
    29221 gaatgccggc gtggagcatg tcggggttgg tgatgacgat gtggccggcc gcgcgaatcc
    29281 tccgccgcgc cgtctgcggc gtgtcgccgt cgaacgtata cgtcccgata tcgactccca
    29341 gcttctggat caggtcgtac agctccgaga gctggtcctg ggagagggcc ttggtgggaa
    29401 acaggtagag cgcccgcgac tccggctcgc gcatgatgcg gtcgaggacc gggaggttgt
    29461 agcagagcgt cttgccggag gcggtggggg tgacgacgac cacgtcgcgg ccggcggcta
    29521 cgtgggcgac cgcctcgccc tggtgggtgt agaggcggtc gatgccgcgg gtcttgaggg
    29581 ccccaatcag gcggccgtcg agggcgccgg ggaaatcgac atagcgggcc ggacgggcat
    29641 cgaacgaccg ccagtgcgtc acattctcag aaatgtcctt gtcggagcgg aggagatcga
    29701 gaacttgttc gaggttcatg gatagccagt ctgggtttgg gcgccggagg agccgatcgc
    29761 gggcgccggc ggcgcggccg ttcacaccac gccggtcagt ttacggcggc acacatcaca
    29821 gaacttccgg cccttgttgt cgatatcgag catcatctgg ctgtagtagt tgacgcaccg
    29881 ggggttgcgg cactcccgca actcgaacaa atgggccaac tggtggagcg cctccttgta
    29941 cagccgggga tacaccttct tctcatcttc gggcaggccg taaaactcct gccgcaactg
    30001 gtacagggac accaccgagg ttccggcgac aatgtcggcg tggccgagca gggtgtcctg
    30061 gtcgggaaga tagaggtctt cttcgcacac cgcgatcact ttttcgcggg aattggcttt
    30121 gacgcgctcg agtttggcca agaggacggt gacatagtac tggctgcgga cgacgttgag
    30181 ggcctcgctg ggcattttca tgcccttgag gatgtcgacg gaccggttga acaccgggcc
    30241 gatgtcggcg gctaaccggt tgaccatcat cgggtcaacc tcaccgagcg ggacgaccac
    30301 gatcttagat ctcaatatcg ccatgaaccc caattcccgg ccgtcggctc gggcggccgc
    30361 aacctgtcgc cgggtcgccc ttcatcggca gccggcggtt gtgcgtcact cggccgtgaa
    30421 aaagcggtcc ctgcggcccg tcggggacac acccacccgc acctggccgg cgcatttcgg
    30481 acaccacccg acaaaggccg aaccctcccg gttcaggtag atgcgcgagt agactcggca
    30541 gcacttgaaa tacatcccga gaaaaggccg gtgatgggcg tctttcggac cgttcatacg
    30601 ctgacaagaa tacggaagaa aaacggaaaa atcaatcact tggaggaggc cctgggggcg
    30661 aaggattcga tcgccgtcag tgagacccgg ctgcggtgtc gccgcccggt tccgggctgg
    30721 ccagttcgcg catgatcgcg accgaggccg agctgcccag ccgcgaggcg ccggcggcga
    30781 gcatggcgcg cgcatcggcc agcgtgcgga tcccgcccga ggctttgacc tcgatccggc
    30841 cgccagctgc ccgagaaaga gcgcgcacct gctccacggt cgccccgccg aaaaacccgg
    30901 tgccggtctt gacgaattgt gcgccgccgt cgatcaccgc ctgcgccgct tcggcccagc
    30961 gggaggcggg gagaaggccg gcctcgatga tcactttgag caggacgttg taggggaggg
    31021 cgcggcgcac ggcgctgatc tcgcgcgcga cgtccgcgca ccgtccctcg accagccagc
    31081 cgatgttggc gaccaagtcg atctcccgcg caccatcggc cgcgcccttc acggcttctt
    31141 caaccttgac gtcggtgcgg ttcgctccga gcgggaaggc ggcgaccccg atcactttga
    31201 cgcccgttcc ccggaggtga tcgccgcaga gggggatcca gacgggattg acggcgacgc
    31261 cgcagaattc gtgggcgcgc gcctcggcgc acagccgccc gatgtcggcc gcggtgacgt
    31321 ccggtttcag cgcggcgtga tcgaagcgac ggtggagctc gtgcaggagc gggtcgctca
    31381 tcgcagtccc acctctccgg cgaaggagtt cccggccagg ctgcccggca ggcggaacag
    31441 ctcggcgacc gtggcgccga cgtcggcgaa ggtcgcgcgg gcgccgagat tgacgccggc
    31501 tttcacccgc cgcccgtacg ccaggagcgg gacatactcg cgggtgtgat cggtgtggcg
    31561 cgccagggtc gggtcacagc cgtggtcggc cgtgatgatc agcatgtcat cctcgcgcag
    31621 ggcgtcaagg agcgcgccga ggcggcggtc gaaatcctcc agggcgaggg cgaaattcgc
    31681 cgggtcgcgg cgatgccccc aggcctgatc gaaatcgacg aggttcgtga aaatgaggcc
    31741 atggtcgcgg tcgccccgga ccagctcgat ggtcgcttcc atgcccgccg cgttgccctc
    31801 gggcttcacg tgggaagtga tgccccgggc ggcgaacagg tcccagatct tgccgaccgc
    31861 gtacgtgggg cggccggccg cgaccatcag gtcgagcagg gtgggtccgc cggggggcag
    31921 cgagaaatcg cggcggccgg cggtgcggac aaaccgtccg ggggagccga tgaaggggcg
    31981 ggcgatcacc cgggcgaccg cgtgctcacc ggtgagcagt tcgcgggccg cctcgcagta
    32041 gcgatactgc tcggccaagg ggtagcgctc ctcgtgggcg gccatttgcc agaccgagtc
    32101 ggccgaggta tacaggataa ccgccccgga ccggagatgc tcttcgccga actgctcgat
    32161 gatggcggtc ccggaggcgg ggatgttgcc gatcgtccgg acgcccgcct ggagctcgaa
    32221 ctctcggaca aggcttggcg ggaaaccgtg cgggtaggtc ggaaagggca ccggggtgat
    32281 gagaccggcg atttcccagt ggccggcggt cgagtctttg ccggccgact gctcggccat
    32341 cttgccgaac gcggccgccg gcgcggcgac cggggggacg ccggcgatgg ggacgatgtt
    32401 gccgaggccg aggcgccggc agttcggcag gtccagcccg ccgaccgcgg cggcgcagtg
    32461 cgggacggtg gcggccccga cgtcgtcgta tgcagcggcg tcaggcaaag cgccgacccc
    32521 gcaggcgtcg atgacaagga tgatggcgcg ccggatatac atgccgccaa agtaggccgg
    32581 cgccggccgg ttgtaaaccg aaaacggggc gcacaaggac gctgcccgcg gcgccggggg
    32641 cttcgcgttg gccgggacca agaccgggaa ccggccgccc ggccgcaggc gtgcgggtta
    32701 cggggtgatc ttccgcgact cgagcgcgta gtcgacgtcc gcctcgatcc gcacatcgta
    32761 cgtcgccgtg tcgctcttct tgacgcgcgt gtagtcgccc tgaatgatcc agcgctcccg
    32821 ctgcagcacc accatgcctt cgcggaacgc gaaatacatc ttgccggtcg tgcggatgcg
    32881 gtcgacgccg aaccgctgga cgctgtcctt cgggttgggc tcgaccggga tcaccaggtt
    32941 gccgtcgacg ttgagcaggg cgcagtcgta gccggcctcg cggacgaagg cgaccacttc
    33001 gtaggttgtg gaggcttcca tgttctcatt gggcagcagc accttggcgg tctgggtcca
    33061 ggagaaaccg ggggacacct cgccgggcgg gaacaccggc atgccctgct ccatgaagtg
    33121 tttgatgtag ttctgcgagg ggaggtcgtc gctgtcgtcg aacttgacat cgaggacgtt
    33181 gccgtcgctc tgcatgagga tgcgcatggt ccgctccatg gacaccgagt cgcgcttgag
    33241 ggtgtcttcg cgcgacggca ccgtgtagga ccaggagtcg acctcgcgca tgagggcggt
    33301 gttctcgtcg cggacctcct cgatcgtctg catgacgcgg accttgaaag cggtgctgct
    33361 cttttcgatg atcgagtcgc cccgggcgac caggacgttg cgcttcatgt cctggttgta
    33421 gacaagctga agtccggggg cgaacttgta gcgcaacagg acggtcttct cgccctgctg
    33481 gcaggccgcc agcgcgagca cagcggcgag gatgactgcg gagaggcagg cggttagtct
    33541 ggtcacaatt ccgagactcc ttcccagagg ctattacgat tcgtcgagcg ggttggtttt
    33601 gactatctgc ggcagggcgg tgaaacggcc gaggattccc ggcccgccga tgagcatcat
    33661 gccgcgggtg atccgggccc gatcttcgcg ggtgaagacc gggctgtcgc cgagctcgga
    33721 gcgcacctcg gccgtcgcca gcacgggaat ctccgggccg atcgtccggc tgatcaggag
    33781 ctcccggctg gatccgaggt agatggcggc gaagaggatc gccagcgagg ccgccaccga
    33841 caccagcgcc cgcaggaggg cggtggagga gcgcggcgcg gcgccttcgc cgcccccgcg
    33901 gcgaaccgcc tctgggtcgc cggccagaac cgtgcgggcg aggatatggc tctcgctttc
    33961 ggcgaaatac tcgtccccgg ggtcgggcgc ggcggccgag ccgagcaggc cgcggacttg
    34021 gacggcgagg tcatgttcgc gccggcaggc tgcgcagtca gccaggtggt cgcgcacgcg
    34081 cccggcggct tcggccgaca gctcgccgtc gagaaagtca tccagccgcg ctacgacatc
    34141 agagcaggtc atctcaactc cctttcctca ggcgcttctt cagggccttt cgcagtttgg
    34201 atctggcgat atggagattg gagcggacgg tcgcctccgg gcaacccaac atcgccgcga
    34261 tctccgcctt cgaataccct tcgatgtcgt gcagaatggc ggttagttgt tgtcgcggag
    34321 gaagctccct gagcgcccgc ttgatctcct ccagcaaagc cctgttctcg aattcttcgc
    34381 tcggcgtggc cacctgccgg gccagatcga tctggacctc ccccggcagg gaggacgaat
    34441 cttccggcat ccggctgtgg cccctctgct tgcgcaggac gtcaatggcg tagttggacg
    34501 cgatgcgcag caggaaggtc gagaaaaact ttacttcggc cagctccttt cgtcgtcgat
    34561 aaatgcggac aaaggtctcc tggacgactt catcggcatc caggtgattg ccgagcacct
    34621 ggtaggccag ggagtagacc ttccgctggt accggtggac aagttcagtg aaggcggcgt
    34681 cactccctcg gacgaaatca tccactaact ccgggacagt cttatgcata atccccgaag
    34741 tcatgatgta tcgaagagac gtacggcatc gccacctgtg caatcatcgg ggtgaaaatt
    34801 ctgttgacta tccccgacgg caagcatatg cttgccgctg aagtttgtca atctttttta
    34861 cggcaacaac ttatacccgt gggcagttca ggcgaacatc agcgcatcat cgaggcgcat
    34921 cgacaccgat tctttctcgt gggggccata gggaccttca tgtcgaccct ggacgggtcg
    34981 atcctcaacg tggccctgcc cacgatcgcc gccgacctgg gggcggacat tcacctggtg
    35041 gcatgggttg tcctcgccta tacgctgacg atcatctcgc tcatgctcat cttcggcgcc
    35101 tggaccgagc gcaaggggta cgacttcgcc taccggttcg cctactcgtt tttcgttctc
    35161 gggtcggcgg cctgtgcgct gagcccgtcg atccactcgc tcatcctcgc ccgggtagtg
    35221 caggggatag ggtcggcgat gtttcaggcg gtcggaccgg gtctggtgac ggccgtcttt
    35281 ccggcggaac agagggggag agggatcggg atgatggtga tgatggtggc ggccgggctg
    35341 atggccgggc cgccgctggg cgggttcatt ttgcagtttc tcccgtggca gtggattttc
    35401 gtgatcaatc ttcccatcgg cgccgtgggg atcgcgctgg cggcccgcta tttccggctc
    35461 ctaccgaagc cggaggacga tcgcccgatc tacctggccg gcggggcggc gatcgcgacc
    35521 gccctggtgg ccggcacact ggcgttgtcg atggtttcgg agcacgcgct gcgcgactgg
    35581 caggtttggg cgccggcgct ggcggcggcg ggggcgttcc tgctgttcct tcgctacgag
    35641 agccgcccgg cgcgccacat gatcggcctg gacatgttcc ggaaccgcca gttctccacc
    35701 gcgattgcgg cggcgatcct gatgttcgtt accatggccg gcggggccat tctcctgccg
    35761 ttctattttg aaagcgtcaa ggggtacggc cccagccgca tcggcctgtt cctcgtgatc
    35821 atccccgcca tcatgttcgt ggtggcgccg ctgtcggggc gaatctccga ccgcatcggg
    35881 tctcttttcc tcacgacgag cggagcggcg gtggcggctt tcgggcagta tctgctgctg
    35941 cacctggatg ccgcttcatc agacgctttc atcatgctcg tgctggcggt catcggcctg
    36001 ggcgcggcca tcttcaacac gcccaactcc tcgtcgatca tggggtcggt cggaccgacg
    36061 gagcgggccc gggccagcag cattatcggg accgcgcgca acatcggcaa ctcgatcggg
    36121 atcgcaatgg cgacagctct cttcgcctat tttcggtcgc ggcgggcggt cagtctcggc
    36181 gacccggccg ccctgttcgt cgcggccttc catgatgtcg tgaaggtctc gctcggggtg
    36241 gcgctgttga cggtgccgct gtgcctcacg cggcacaacc ggccgcgtcc gggacaaggg
    36301 ggcggagcgc cggagcctcc cgccgccgga gtctgacagg tccgccggcg ccgtaaaatg
    36361 cagttgacaa cctctcgggg agggcgtata ctggcgaacc tacaggtcat ttggggccat
    36421 agctcagttg ggagagcgct tgaatggcat tcaagaggcc gggggttcga ctccccctgg
    36481 ctccaccatt tagaaaagac aaacccgtct ctcggctcta ccccattaac gggggacgga
    36541 catccatgcg cacccggttg ataatgccat cccatccaca gtgggtcagc actcgcaacc
    36601 ggtaattctc aaaatttctg aagccgtagg ctcgcctgga gatcatctcc atcttgttgt
    36661 ggaagccttc ggtgataccg ttgctcttgc tgaatcgcca catcgcaacg ataggctcga
    36721 gccagctggt cagcgttctt gcgagaacgc gcagagggct ctccagaagc tgctccagta
    36781 gcgctgtaag ctgcggcagc tttttcctgg cccgcttcgc cgtgacggtt ttcaacagca
    36841 tcaggcgaac cagcctctgc ttcgtcacgt acagctgctg tagcaccggg taagcggcca
    36901 ggtagtgcat caggttggcg tgctgctcgt tgctcaggtg ccactgatgg cgacgcatca
    36961 ggctgatcaa gccacggtgc ttgcgtccct ctgggtcgtg cagcttccag accctcagaa
    37021 agtgttgatt gatcaggcgg acgacatgaa agcggtcagc cacgatgatg gcgccggaga
    37081 agtatttccg ggcaatactg cgataggtct cagac
//
LOCUS       DAOPOE010000019        35505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1588_length_35505_cov_5.490381, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000019 DAOPOE010000000
VERSION     DAOPOE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 35505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35505
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_35505_cov_5.490381"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..587
                     /locus_tag="PKM94_04375"
     CDS             168..587
                     /locus_tag="PKM94_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65907.1"
                     /translation="MTESFFRDDNAAADAAAPERLQQSAEERAARRRGIDEGEFETEE
                     GRLAAVMAYIPILCLVPLLNIRDNKEARFHARQGMLLFLVEVVAVLFLVDGVSSFVFR
                     VILILAVGLAVAGVALALQGRGIRLPLLGDLAEKVKL"
     gene            1098..2042
                     /locus_tag="PKM94_04380"
     CDS             1098..2042
                     /locus_tag="PKM94_04380"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD65908.1"
                     /translation="MDKRTLNRIIVGWILLLILAALIGKVTVAAPNGGQTAADFLRIG
                     FGARSAGMGGAFTAVSSGAQAAYWNPANLWTVTSGEAVLSHFAWYQDVTVEQGAVAHT
                     INEKSSLAASIGFLNYGTIDGRDINGLATGDVTAYDWFGAVSYSYDLSPAVSLGLTGK
                     FVNQKLDDLSASSFAADIGGAYRFDKVTLAAVLANVGPDMSFENVSEHLPSSARVGAA
                     VALFDRQVLAAAEMEKPFYGDWVFRQGLEYGYRDQYFVRAGYNYYPGAEDRSFGSGMS
                     LGAGVQFSRLGIDYAYTVQEHYTSEDLHRFSLEFRFGD"
     gene            2485..3339
                     /locus_tag="PKM94_04385"
     CDS             2485..3339
                     /locus_tag="PKM94_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD65909.1"
                     /translation="MSSVDVSLDHAVATPYLGLVEQVKYFACEYLHGLLFVACSMLFV
                     MAFPAALTKPAKVKAMVNRGVKRTLDIVGAIIGLTLSLPIFLIVPILIKLDSPGPIFY
                     TQTRIGVNRRRRDRRYHQRSEIDTEQRQRNRRRQDLKGKPFQVVKFRTMVANAEQLSG
                     PVWASKGDPRITRLGQFMRKTRLDEIPQLLNVLMGDMSLVGPRPERPTFVAELSSKID
                     NYCDRLQVKPGLTGLAQVECGYDTDLASVVKKVSYDLAYIENWSIWLEIKILLRTVVV
                     VVTGRGAN"
     gene            3505..4218
                     /locus_tag="PKM94_04390"
     CDS             3505..4218
                     /locus_tag="PKM94_04390"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOD65910.1"
                     /translation="MILAAALAPVPVAAQADSDPGVIVRTDPPGCLVTLRGEIQASGV
                     APVHFRQLLIGDYRLKVERPGWETYSSRLVLDPTKQVAVDVNLSRKTRFKGAVRSLVI
                     PGWGQRYADKDLKAGVSFGMALASGAAFLLADDEFDYRHGVYNEEKRKYLALPKDAPA
                     EVQQAMFNSVKTAHEKAVDAENVRRATIGAAIGVWALSLLDAVLFFPSEQGTFSIKGV
                     AVAPGRVSDGYGVTLSGRF"
     gene            4283..5545
                     /locus_tag="PKM94_04395"
     CDS             4283..5545
                     /locus_tag="PKM94_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD65911.1"
                     /translation="MARFIGLLIVLAVLGAAGCERYLDSTDPGVADPGGMATPRITSA
                     AMLDGAVILTWRVDNPRGIVRYRIYATDATDTAGGSYLLRDSTTATTDTITNLPASQW
                     YLFRVAAVRSTGYEGPRSEPFAASVQHLSIAINDGSEYTSNRTVQVRVNAPAGFATHI
                     MMSEAPGLTDAAFTPLFGTAAAFTLSTGDGVKRVYAKLQFEDGLVTAAALVDSITLDT
                     QAEIDSVYFGDPGADGYFGLGDVVTFGLAAAGERGGAAAVSFAGAGTVTLADDGTGGD
                     DAAGDGRYHGRWTVPAGFTLAAGLVTGTFTDAAGNRRQAAAPRFLTVYTPPAPVTATA
                     LATGPTTVALSWTSSLSSIFAAYRVYRSTSSGVGNEATLLTTITAQATTTYDDTGLTP
                     GTTYYYRVDTVDRSGLAARSLVVSARPQ"
     gene            5569..6414
                     /locus_tag="PKM94_04400"
     CDS             5569..6414
                     /locus_tag="PKM94_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65912.1"
                     /translation="MGIVDGINRLIGLILDVFRQIGRGRIWLFLLVYFAVAWFFLLAL
                     HRFTSPLFYSAVTAWTTLIDPQRATGFTHYRGHYLLLPFFFDWAKLIISILFEGVIFG
                     SAAVLFYERFVQVDEEDRFRFRTVARLWIHLVLALVIMNGVILAVNVGLAKVLHPLLE
                     YSPRRQSAFRWVGLPAAYVCVAGLFFYLVPAIAVYGENIIDGLRRTVRVFLRNPVTSV
                     VMAGLVLAGPILLSNIVSSPDTLVDRFRPELVYYLLVAGLFAEAVAGFFWMGLSVRML
                     VDQEE"
     gene            6622..8052
                     /locus_tag="PKM94_04405"
     CDS             6622..8052
                     /locus_tag="PKM94_04405"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD65913.1"
                     /translation="MKNILLVDDDKELSQSLANLFDSDKFRFRFLEDGTEVPQFVTRH
                     DDIDVVMLDVNLPSMSGLEVLKQIKQVRDQLPVIVISGFVSTENAIEAMREGAFEYLT
                     KPFQIEKLIDTVTKACGYSAPGKRPPAAAPESAVDAMQGEIVGQSPEIVEIAKLIGQV
                     AKTDAAVLIFGESGTGKELVAQAIHRNSNRRHNDFLSVNCAALHETLLESELFGHEKG
                     AFTGAYFKRIGKFEQCAKGTLFLDEIGDMSMLTQSKLLRVLQEKRFERVGGNETIEAD
                     VRIIAATNKSLVQAMKEGSFRVDLFYRLKVFSIYLPPLRDRRSDVPVLLDHFIRKFSK
                     QLHRPPKPVSKRAMQMLVKHHWPGNVRELENNIQTALVIAKGDELECEDFPIFSESTG
                     RVEVNLNDLQEDYTETFRRIIEPVMPKLINNNPGQVFHFLESAFERAVIASCLKEFEG
                     NQVKTSEALGISRNTLRDRISRYGIY"
     gene            8212..9327
                     /locus_tag="PKM94_04410"
     CDS             8212..9327
                     /locus_tag="PKM94_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOD65914.1"
                     /translation="MRICHLGDSHLGAGANHPRRGASGLTLRQEDIIAAFAEAVDKVI
                     ALRPDVCIHAGDLFDAVRPLNRVMAAAGELLHRLAEEAGIPTVLISGNHDAPKQPHVG
                     APIDVYRQIDNLYIASAGRREVFRIGEAAVHALPHCLTGPILQEELAQCRPEASARYN
                     VFVGHGVVAGMPEFAMADLGEQEIEVGKLEGFDYIALGHYHNNCEVGKRAWYCGSTER
                     LSQAEREAEKGFLEVVLDPFEVRFHRVHARPMVDIPPIDATGKRGDQLAEMIRAQVTA
                     AGAEDKIIRVRVEGVTEETLKTMPSDVLMELKQQSWALDLTFQKAKAEGAGEAFGRAA
                     IGRLDQEFLRFLESTEIRGFDRDRLREMGLAYLAEEE"
     gene            9529..10179
                     /locus_tag="PKM94_04415"
     CDS             9529..10179
                     /locus_tag="PKM94_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65915.1"
                     /translation="MKKVLAAALLAALLAAGEAMAISAQGSGFGTLATARGIGAGRAS
                     FAGGVGLADATTAFGMVRYGFADYLEGRFKLGLRDDAGMDAVLQIGADVTYQFWSVGP
                     QSPNPMDLAFGGMIEYFPPDYLDDVDESLWMFGGYAIGSYPFALNNGMKLSPYGRLNL
                     RVESFGTDVRGGDNSELEFGLNGGAMLEITPTLNAYGEFQIDGNDGVFLGLEFLIM"
     gene            10221..10994
                     /locus_tag="PKM94_04420"
     CDS             10221..10994
                     /locus_tag="PKM94_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOD65916.1"
                     /translation="MTYLDALILGLIQGLTEFLPVSSSGHLVLAEEILGVKQPGVAFE
                     VLVHLGTLVAVTIYYRRRLWALGRAVFDRTMGSERKMILYLIIGTIPAGLAGVLLEDF
                     FERTFGNPIETSIELIITGVILLITRYFLRGKKPVAAGNALIMGVGQAVAIFPGISRS
                     GTTIAAGIFRGVSPAAAAEFSFLLSIPAIAGAIVLKVGELSDVSAALLGPYAAATVTS
                     FVFGWAAVSFLLAVIRRGKLDYFAYYCFAAGALGLYIFL"
     gene            10996..12588
                     /locus_tag="PKM94_04425"
     CDS             10996..12588
                     /locus_tag="PKM94_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOD65917.1"
                     /translation="MEKILVIRFGSLGDVILTSAAVLNLKISFPGSRLTFFTKERFGG
                     LAAAFDGVDEVVCLPEGGDGRELVRLALELDRRGFDAVADLHGNFRSWLTRKMVTAPH
                     ATVYPKRRLARRRLLRGRNRLAPGPHTIDLYNQAVRAIGGKTPARRPLLPPPVPDADL
                     PPSVREFLGRHQVCVAAAPGAAHRPKQWPVERFAAAAAELHRTYGAGIVWIATSAEQG
                     KGPKPPAVAPEHCVELVDRPIGEIAAVLRRCTATLANDSGIAHLSSAVGTPAAVVFGP
                     THPALGFAPRGLCDRVLEVEEYCRPCSLHGEKACFREEPYCLTRISPEAAAEAVADLI
                     ARRQAERPAILADRDGTVMADKEYLGDPAGVELVPGAAESLRAAQARGYRIVILSNQS
                     GVARGLFAIEAVERVNARLRDLLARAGVRADGMYYCPHYEDGAVREYAAACGCRKPAA
                     GMAEQAARELGIDLRRSSVIGDKADDVNLARVMGGRGVLVRTGHGAAEEAKIRAAAGA
                     AGIAVAEDLAAAVKLVMDGDCA"
     gene            12585..13574
                     /gene="amrS"
                     /locus_tag="PKM94_04430"
     CDS             12585..13574
                     /gene="amrS"
                     /locus_tag="PKM94_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD65918.1"
                     /translation="MIRAAAYSEALPGGGIVCTLCPVRCRFREGRRGVCGSRFMRDGE
                     LLVDNYGEAVTLALDPIEKKPLYHFHPGTDILSTGPNSCNLGCRHCQNWAISQERAET
                     KFVSPESLAAAARRYGAFGVAFTYTEPLMWFEYIRDVAPILRQAGLAVVLVTNGYIEA
                     APLEELIPLVDAMNVDLKGMAPSFYRTVCKGRVEPVLHTIRRSAEAGVHVEVTNLVIP
                     TLNDCEEDIRKLTAFVASVSERIPLHFSAYRPEYKMRLPATPAGTLVRAREIAQERLW
                     YVYLGNVSVPGGGDTLCRGCGKSLVERRGYRTEIIGMDGSRCSSCGFEAGIVR"
     gene            13711..16491
                     /locus_tag="PKM94_04435"
     CDS             13711..16491
                     /locus_tag="PKM94_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65919.1"
                     /translation="MKWVWTAVLAAAAVGLWLPAQAQFYFGKNKVQYTRFDWQVMTTE
                     HFRIYFYSEEEEIARIAAQLAEDSYRALAAKFNHEINEKTPLIIYSSPSYFSQTNVIP
                     SLLPESVGGFTEFMKGRVVLPFHGSYYDFAHVLRHELVHVFQLSKMEAVLARMGRVNF
                     GRPPLWFTEGLAEYWSKAWDAEAEMVVKDMAVNELQLGIEDLYRVAGSYYMYKLGESI
                     CTFIDSTYGPEKLTLLFENLAKGKTFDEVVKLTLGDDLREVSRKWRYSLKKCYYPQLE
                     EAGLPDMESVQITRDGYSVRGVPIMWDAGDGRREWIVFKANRMGYSGIYMTPSGEGRG
                     RRVKTLLKGERSSDFESLYLLRSGIDATDSGMVVFSSKSKGNDVIYVYNLREERVTHR
                     YEFADLAGAHSPRLSPDGTQLVFAGVTLGGMADVYALNLADGAYRPILEDIYYDTDPV
                     FSPDGNQIVFASDRGPEGKEGALNLFSYALGSGTVRQLTFGRYRDQTPDGAAGGIYFT
                     SDRNGTFNIYRLDSLGGLTRQSTYVTGAFEPRVTPDGKGLVYSGYQKMGYQVFRMELP
                     AEPAPVAQETPPPTPSWRPDRIDSKYVKSSVKYETDYSLDIAQSAVGYDPVYGSLGGF
                     QFAMSDMLGNHSYHFLLTNTANTKDEFLQSFNIGVTYLNRERRLNWGIGAFHLYDEYF
                     NDYDGFYWERQAGMIGVFSYPFSKFHRLDVTTVGRHSNREEGTRTRDRRAVLMTNYLS
                     WVYDNSLWDISGPIDGRRYNLTVGLTTRLDGVRSFNRLAAADVRHYFRLGRVSAFANR
                     LFAYWSEGTEPQRIYFGGSWSFRGFDRREWYTRHVLFASNELRFPLINVLSIGSPIGG
                     IGFRAIRGAIFADVGSAWEEEFDQFYGSFGAGFRVALANLILLRFDFSRTTDFETISR
                     NTEFDFFFGWNF"
     gene            16491..17807
                     /locus_tag="PKM94_04440"
     CDS             16491..17807
                     /locus_tag="PKM94_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD65920.1"
                     /translation="MAPRLLTILAAAAAMLAAACSSSLQLSPENLQEGSPWPYYHGGA
                     SATGCAPAGEFAGRLDVIWETSMAGKPAGPLTLHGGSLAVPSTRNRVEMYETGTGRRS
                     GKIKTKGPVQSGLVAEGGYGYCAVSIGNRLSCFDLRSGDEQWKRFVKETARGAIVAAN
                     RLVIGSATGRLTAYDPADGREVWTFEETQDRFMAPPAAGHGLVFQPGVSGFLYALDPN
                     DGTERYRVKVDGPMVGGVAVAELVVGADMPGRVYGIDAQDGTVRWRTDIGAPVWTKPA
                     VAEGRVFVGHSGGELVALNLVDGRVLWRFQAFEVIAASAAVIGRFVAVGTLGGNVYVL
                     DAASGSVLSRRKLEGRIDQAPATDGDRLYLATDRGRVICLGTLPGVAAEQAEAQADDG
                     TESPEAGASGAHRDNPGLTPPAGWTAAGGKAGRAGGGPPGSKAVKR"
     gene            complement(17875..18546)
                     /locus_tag="PKM94_04445"
     CDS             complement(17875..18546)
                     /locus_tag="PKM94_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD65921.1"
                     /translation="MLRHIALTGICVVLIAALVGCGMSKTQKGALIGAGSGAVVGGLV
                     GNAAGNTAVGAIIGAAVGGAAGAYIGHYMDQQAEEMQKDLEGARIERVGEGIKITFAS
                     GILFDVNKADLRPEAQQNLVNLAAILNKYAETNVLIEGHTDTTGSDSYNLTLSRSRAE
                     SVSSFLAAQQVVSNRFIIQGYGEQQPIATENTAAAMQANRRVEIAIYANDKLKKAAEE
                     QVQEG"
     gene            18938..19363
                     /locus_tag="PKM94_04450"
     CDS             18938..19363
                     /locus_tag="PKM94_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD65922.1"
                     /translation="MRSVMIPNRLAREIDSMFNTFISFPQISAECACDFMPRVNIRET
                     KDDVRLVFELPGIDKQDIKVTVKENVLTVSGERKTVDDENAEVVRREILTGAFSRSFT
                     VPDTIDTDKIAADYRNGMLEVKLAKKEAVKPKEIEVSVG"
     gene            19517..21427
                     /gene="dnaK"
                     /locus_tag="PKM94_04455"
     CDS             19517..21427
                     /gene="dnaK"
                     /locus_tag="PKM94_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD65923.1"
                     /translation="MSKIIGIDLGTTNSVVAVVEGGEPTVIANSEGGRTTPSVVAIDR
                     NGERLVGTVAKRQAVTNPLNTVFSVKRLIGRRFDEVQAEIANFPYKVNRGPDESPVIE
                     MAGQTYKPAQISAMVLGKMKKTAEDYLGQAVTKAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTAAALAYGLDKKKVGKVAVFDLGGGTFDISILELSDGVFEVLSTNGDT
                     HLGGDDFDKRIMDWLIEEFRMSDGIDLGKDPMALQRLREAAEKAKVELSSTLQTAINL
                     PFITADQNGPRHLNVTLTRARLESLVDDLLERTKRPVQNALADAKLTVDRIDDVILVG
                     GMTRMPKVQQIVKEFFRKEPNRSVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTPLSL
                     GIETLGGVMTRLIERNTTIPTRKSQVFSTAADNQPQVDIHVLQGEREMAIDNKTIGRF
                     ILDGIPPAPRGVPQIEVTFDIDANGIINVSAKDTATGRAQNVRIEASSGLSEQEIARM
                     VADAQQHEAEDREKKRRVEARNRADAAVWQSDTQMKELGDKLSQEQTAALGAATERVR
                     AALKGDNADEMSRAAEDLQTAWQQVSADLYRQAAGPTAGAQPGGNGSGPQTGAAGRPD
                     ADAVDADFEEVK"
     gene            21539..21775
                     /locus_tag="PKM94_04460"
     CDS             21539..21775
                     /locus_tag="PKM94_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65924.1"
                     /translation="MDSLSLGVIGGLLAGICLFAGWVIKRHFVDQRWAAMGRSVYAAW
                     QNGDERHRIEYVNYLEEDAREEDKEGEFRPGRQV"
     gene            21869..22831
                     /locus_tag="PKM94_04465"
     CDS             21869..22831
                     /locus_tag="PKM94_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOD65925.1"
                     /translation="MAKDYYDILGVKEDASPEEIKKAFRKLAMKYHPDRNPGNAQAEA
                     RFKEISEANETLSDPKKRAEYDTLRKYGAFAGAGQGFDPGAHGNGGFDFSDLFRQGAG
                     GRGGFQTFRFGGGVEGLDEILSQFFGGRGGFGTEFGKERVRPRRARQARGTDLKATVT
                     VTLREAVAGTRKRLRIRQTGRTIQVTIPPGIENGGVIRLAGQGMPAPARPEMPPGVPG
                     DLLITVKVMADHQFERKGNDIYTKVTIPLKDAILGGKARVRTPLKEIQVTIPPGTQPG
                     TKLRLKGMGLSVGGVQGDQYVEVDVRIPRAADLTEEQRKMLEEW"
     gene            22887..24125
                     /locus_tag="PKM94_04470"
     CDS             22887..24125
                     /locus_tag="PKM94_04470"
                     /inference="COORDINATES: protein motif:HMM:NF027613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD65926.1"
                     /translation="MYVVLDTNILMADYLLRGRWFTDLRHFLRLNNGKLVIPRIVLEE
                     TVANYGRDYKAIISEAKRLDKRLKSLSKRNSLDYERLEQESNREMNNYREFLLRELEH
                     WNLDAASEKPPGVDDIITAVVSRRKPFKENGEGIGDYLLWRAVTQVATNSEPTVLITN
                     DTSDFGNQMDPVGLHDALIQELGENAPKVSLYRSIQSFIEIHYPKPPWATEEYLRCLP
                     GFQRVVSLVNAFVKRMPGEFVFPAGLRLIPSEPAVKSFYVYQSGDDAWTTVLRFEVEA
                     SVEGLAPSRVAKPVEGLVPLAAFSGNVTVLASVHCEVRNGDIRLVSTLEREVTREDLR
                     EGTLRYIRRSTAMYSEAASGGVLLSGIYTAPDFRQYPLPYGAVHADPVRPMPPIKPPS
                     APAVLWKRSQPSDGDAKKGD"
     gene            24167..26797
                     /gene="clpB"
                     /locus_tag="PKM94_04475"
     CDS             24167..26797
                     /gene="clpB"
                     /locus_tag="PKM94_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOD65927.1"
                     /translation="MNPNRMTQRSIDAISYAQQQAAAEQHPQITPEHLLHALLVQEEG
                     LVPQLLKKTGAETGAIAGEVDEALKMLPHAAGGQIYPSRELSQVFTRAEAELARFKDE
                     YVSVEHLLLALLEVRSKAQEILKGHGVRRDELLKALSSIRGSYRVTDETPEAKYGVLE
                     KYARDLTQAAREGKLDPVIGREEEIRRVIKILSRRTKNNPVLIGEPGTGKTAVVEGLA
                     QKIALGEVPETLKERRLVALDIGALIAGSKFRGEFEERLKAILKEVEEAEGEVVLFID
                     EMHTLVGAGAAGPGALDASNMLKPALARGEIRFVGATTLKEYRQHVEKDAALERRFAP
                     VLIEPPTVEETVSILRGLRERYEAFHGIKISDGAIVAAAKLSDRYIADRFLPDKAIDL
                     IDEAAAELRIAIDSLPEELDAVEKKIRQLEIEKEGVKNEPDARERLKPIEEVLKDLYA
                     QRNDLRTQWTREKDTVEGIRAVKVEIEQLKHRAAEAERKADYETAAKIKYGQIVEAEK
                     KLQSLTGALAAIQEKRQLLKEAVDEDEIAEIVARWTHIPVARLTESESEKLLRLEEEL
                     HRRVVGQDEAVAAVADAVRQSRAGLSDPHKPIGSFIFLGPTGVGKTELARALAEFLYG
                     TEEAIVRLDMSEYMEKHSMSRLVGAPPGYVGYEEGGQLTEALRRRPYAVVLLDEIEKA
                     HPEVFNILLQVLDDGRLTDNQGRTVSFKNAILVMTSNVAAEYIQTETAGMGEFNRDMI
                     WENIKKNVLDAVRRTLRPEFVNRIDEMIVFHALTPAEIRAIVRLQLRRLERLLAEKDI
                     GLSVTEAAVGQIAEAGYDPAFGARPLKRYIAKELAQDIAKQMLAGTVRPGQTLRIDFA
                     GGEMRFTAGPTAEGSGPQGS"
     gene            complement(26895..27884)
                     /locus_tag="PKM94_04480"
     CDS             complement(26895..27884)
                     /locus_tag="PKM94_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD65928.1"
                     /translation="MHRRLLPLTALFSVLCAAPLRAVEEHVEEVEPTVWDFLFTWHYM
                     LFVVIAVIGMILVMNRLVNKWVRIGAMAAVFVLFGLDYLFPLHPSPMCGVVNLIMFRI
                     TTGMFFPIFVALALAMFVPSLVGRKLFCGWVCPLGALQELANKIPFKWKFKQFNFTAF
                     NAVRLSLFVLFILTVFAVKNHVGLLAERLEADTSVGPWTAFSAYSVYDPINFFELLHW
                     SVDATFIVMMAVLLLAGLVLYRPFCYGICPIGALTWLCEKIAPARVRIDREACTECMD
                     CVAASPCPTIKQIVEGKVKVLADCTSCGECIKACEYNALSFGFTARRGKPVQP"
     gene            complement(28057..28650)
                     /locus_tag="PKM94_04485"
     CDS             complement(28057..28650)
                     /locus_tag="PKM94_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65929.1"
                     /translation="MLPILVFSALLSAATANPFADLIEPGLDSNTAFYEGAARTYVFS
                     PPAGYRLVLEEAAAAGLSMAYIPGDQDFDTADLLVGITIYKVDSAATSFAGIIAEDTA
                     AIRSHYGGRIEMWPVDSMCNAQGEIIPTVYVNDPAAFVPTVMVSYYNGGAEIIILELV
                     ISPRQPRFKAEAVFELILERFKSLRRGDPSKIEFGQG"
     gene            28891..29940
                     /locus_tag="PKM94_04490"
     CDS             28891..29940
                     /locus_tag="PKM94_04490"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOD65930.1"
                     /translation="MAGIVGYGCHLPRHRIKVEEIAKVWGADAPSYKKGLMLREKSVP
                     PPDMDTITLSVEAARRALIRAQTVDPKDIGAIYIGSESHPYAVKPSGTTVGEAIGATP
                     DLHCADFEFACKAGSEAMFVALSHVESGFMKYALAIAADTSQGAPSDALEFSAAAGAG
                     AFIMGKDNLIAECLYTHSYMTDMPDFWRREHEFYPQHGGRFTGEEAYFAHTKGATTAI
                     LKKSGMKPQDFQWAVFHQPNGKFPQRVALELGFTQAQIDPGWLSPRLGNTYSGASPIG
                     LTATLDTAKPGDLIMMCSYGSGAGSDAFIWKVTDRIDKVRDLTIRTHKLLDENLTYVD
                     YGTYAKFRRKIRKNA"
     gene            29952..31112
                     /locus_tag="PKM94_04495"
     CDS             29952..31112
                     /locus_tag="PKM94_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOD65931.1"
                     /translation="MRTVCIAGVGMSQWGEVWRTSLRQLFADAAGEAIKNAGVDHLDS
                     LYVGCMSGGLFVQQEHLGSLMADYMGMPGLPAMRVESACASGGMALRAAYFEVAAGAA
                     EIVMAGGAEKMTDCTGDQATAALATAADQEYEVFNGATFPGLYAMMAHAHMARYGTTR
                     AMLTAVAVKNHRNGSMNPFAQYPFPVKPETVESSVMVADPLRILDCSPITDGAAAAIL
                     CSEEVAKKLGRPYVRIIGSGAATDTIQLAQRADLTTIKAAALATARALQQAGRKITDV
                     QFAEVHDCFTIAELVVLESMGVYKPGGAGPATLAGETALDGKFPVNPSGGLKSKGHPV
                     GATGIAQAVEVFKQLTRQAEGGRQIKGNLRIGMAQNMGGSGGSSVVHIMEVA"
     gene            31112..31513
                     /locus_tag="PKM94_04500"
     CDS             31112..31513
                     /locus_tag="PKM94_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOD65932.1"
                     /translation="MAMFSSRNWREYPQRYRLEAVRFKKSGKTYFPPRSIDPENRDTE
                     RETVRLPDTGKIVTYTVVRVAPSQWGDMAPYALAIVQLTDGTRLFGQMTDCNVDEVKI
                     GMEVRIEFRRIQTEEHHGVLSYGYKFVPKWW"
     gene            31717..32211
                     /locus_tag="PKM94_04505"
     CDS             31717..32211
                     /locus_tag="PKM94_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65933.1"
                     /translation="MLGAVAGDIAREIAQIKQEAAGRGIRVMRGRLIGPGGAEAPRVV
                     WVADGDGDWREFLDFAQSVGVKAVVIDFDRIDMELLRADVEDAAVDEEYEEGRTKMLQ
                     MLEKLDKHDGEIGHVGIIFPHGPLVYQASVRTPWYDDYVTVREIAGAADEFAEEEEDP
                     FDIQ"
     gene            32545..35139
                     /locus_tag="PKM94_04510"
     CDS             32545..35139
                     /locus_tag="PKM94_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65934.1"
                     /translation="MVRSVARFVAPAAVLVSALAAWGGSVQADEGIAYHDLRDRAISA
                     WQEVDTFTFAGQVGDVVLIRVNRTAGELNPLVRLYRPDGSLLAEAGPLWSAYDRTEIL
                     DQALPQGGSYRLCVADDDGLQWGDYAVTLECVNRPTGAETAAYDDVRSDTLTAVPEIK
                     LYRFSGLAGDRVILRANRTAGELNPHLRLYRPDGSLQATADPMWSAYERTELFDQGLT
                     QNGTYTVCVIDDDGFQLGELAFTLQCVNRPVGAEDMVYHGLVSATISAVPEMELYRFS
                     GQIGDRVVLRVNRSSGELNPHLRLYRPDGGLVAEAGPVWPGNDRTELFDQSLLQTGQY
                     TVYVSDDDGSQLGEFALTVECVNRPLGAKAVVYDDLLEDTLETAPRFRLYRFEGQIGD
                     RAIFRVNRTSGEVNPCVRVYRPDGSLLAEAGPLWPNYERAELFDLNLSQTGTHTLLVA
                     DEDGYQLGQYHVTLQCVNRPVGAVTAGYGELVSGQVADVPEMYLFRFDGAVGEQVCIR
                     LNRTSGDLNPWVRLYRPDGSLLAEAGPLWPGYERTELYDQLLSQTGTYTLYVSDEDGY
                     QTGSYAMMLECINDPGEVYPAAYNDLIADTVDAVPRWHVYRFAGQSGDRLIVRANRIS
                     GEVNPWIRIYRPDGSLLADAGPMWPSYDRAELYDVTLNFSGTYTLYVSDEDGYQMGEY
                     GVTLQCVNRPIGAGTMAFGDVAAEVIDSFPRIRLHRFLATVGDRVVIQASRVSGDVVS
                     WVRLYDPSGNVVTEGQSDGAVTICAALIPATGQQTIYLSDDDGFGLGTDTLRLLEFKC
                     GDVNTMSGPAAINVSDITYLIAWTFRGGPPPMCLQAADVTNDGGFTVADLTYLISYLF
                     RGGPAPAC"
     gene            35351..>35505
                     /locus_tag="PKM94_04515"
     CDS             35351..>35505
                     /locus_tag="PKM94_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOD65935.1"
                     /translation="MRIGNAGGYWGDDLGALRRQLTGGPLDYITMDFLAEITMSILQR
                     QRKANPDL"
BASE COUNT         6591 a        10173 c        12609 g         6132 t
ORIGIN      
        1 gctggagcgc ggcttcggtc cggtcgggtt cagcgtgacg attctcgacg gccggcagaa
       61 actcgaaacc tggcccgagg gaaagccgat cgcgctagag gtgttcgagc gccaccggga
      121 gccattctgg tcgtgacccc gaacggcgcg cggggaacgg aggagctatg accgagtcgt
      181 tttttcgcga tgacaacgcg gcggccgacg ccgccgcccc cgagcgcctc caacagtcgg
      241 ccgaggaacg ggccgctcga aggcggggaa tcgacgaggg agaattcgag accgaagagg
      301 ggcgcctggc ggccgtcatg gcctacatcc cgattctctg cctcgtcccc ctcctgaata
      361 tacgggacaa caaggaagcg cgcttccatg cacggcaggg catgctcctg ttcctcgtcg
      421 aggtcgtggc cgtgctcttt cttgtcgacg gggtgtcgtc gttcgtgttt agggtcattc
      481 tgattctcgc ggtcgggctg gcggtggccg gggtggcgct cgcgcttcag gggcggggaa
      541 ttcggctgcc gctgctgggg gatttggccg agaaggtcaa gctctagcat ttttcccctg
      601 cattcttctg ctgccggtca atcggccgtc gtggacggcg gacgcggggc gcaaatgtga
      661 gaacggagaa attttaagtt gatttccgaa ggggagagtc gtacataggc gcgacagatt
      721 gtgcaagcgg gcagtgcacc tgcgcgataa ctgcgagcgc gggtggaaat ctgacaccac
      781 ggtgctctgg ttctgaacag gtcacacatc aggttgtgtg ataggttgtt ggccgagagt
      841 tgcggcgacg gcagcggggt tctgttcaga ggccggtcag aacggtgcga ggcgctcaac
      901 ggcgcccgcc ggtcgggcag cgaagaagct gggcgccgca gcgaaatggg tgggagaaac
      961 ttgttgagcc gcagagcgtt tacttcaggt ggcggccgcg tcgtgacgtt gcacagggaa
     1021 cttctcacgg ccgccggttc ggcatttccc ttgcctcact tcctatggaa catcagaaac
     1081 actcaagagg ctgggaaatg gacaagcgga cactaaatcg aatcatcgtc gggtggattc
     1141 tcctgctgat cctggcggcg ctgatcggca aggtgacggt ggcggcgccc aacggcggac
     1201 agaccgctgc ggatttcctc aggatcggct tcggagcccg gtcggccggg atgggcgggg
     1261 cgttcacggc ggtgtcctca ggggcgcagg cggcgtactg gaacccggcg aatctgtgga
     1321 cggtgacgtc gggggaagcc gtcctctccc atttcgcctg gtatcaggat gtcaccgtcg
     1381 aacagggggc ggtcgcccat acaatcaacg agaagtcctc tctggcggcc tccatcggat
     1441 tcctcaacta cgggacgatc gacggccgcg acatcaacgg attggccacg ggggacgtga
     1501 ccgcctatga ttggttcgga gcggtctcgt actcctacga cctgtcgccc gccgtgtcgc
     1561 tggggctgac ggggaagttc gtcaaccaga agcttgatga cttgagcgcc tcgagcttcg
     1621 ccgcggacat cggcggggcc taccgcttcg acaaggtgac gctggcggcg gtgctggcga
     1681 atgtcggtcc ggacatgtcg tttgaaaatg tcagcgaaca cttgccgtcg tcggcgcgcg
     1741 tcggggcggc cgtggcgctc tttgaccgcc aggtgctggc ggcggccgag atggagaaac
     1801 ccttctacgg agactgggtc ttccgtcagg ggctggagta cggctaccgg gaccagtatt
     1861 ttgtgcgcgc cgggtacaac tactacccag gggccgagga tcggtcgttc ggctcgggga
     1921 tgagtcttgg agcgggcgtg caattcagtc gcttggggat cgattacgcc tacaccgttc
     1981 aggagcatta cacttcggag gatttgcatc ggttttccct ggagttccgg ttcggcgact
     2041 agcggctgaa gtcatttgga gtgatcgagt tggagctggc cagagggccg gctctttttt
     2101 tgtcctcggc ggagctgctg cagggggagt gcaaccaatt gcaaataata tgtggatact
     2161 gtcagcacat gacgcgatgc ggcaggggca cggtcggttc gggggtaatg aaatcgcaga
     2221 attctttacg gcgaaacggc cgttactcat tgggtaataa tggattgcaa atagtcggcg
     2281 gtgaatcggc gcgacgcggg gcgatgtata ggaagttggc aatagttttg ctgagagcag
     2341 atctaaccat gttgcggtaa acagcagggc gtacgaaaac gccgggctct aacgaagaat
     2401 atgagtagtt ccatacctaa tcaccatcat ataacaaatg agagcgccgg agcagtagcg
     2461 cgccgggaat tcgcgtacgg atcggtgtcg agcgtcgacg tttctctgga ccacgcggta
     2521 gcgacgccgt atcttggtct ggttgagcag gttaaatatt tcgcgtgcga gtacctccac
     2581 ggcctgttgt tcgtggcctg ctcgatgctt ttcgtcatgg cgtttccggc ggcgttgacg
     2641 aagccggcta aggtgaaggc gatggtcaat cgaggcgtga agcgcacgct ggacatagtg
     2701 ggtgccataa tcggcctgac gctctccctg ccgatcttcc tgattgtgcc gatcctcatc
     2761 aagttggact cacctggtcc gattttctac actcagacgc ggatcggggt caaccgccgc
     2821 cgccgcgacc ggcgctatca ccagcgctcg gaaattgaca ccgagcagcg gcagcgcaac
     2881 cgccggcgcc aggatctgaa aggcaagccg ttccaggtag tcaagttccg gacgatggtc
     2941 gccaacgccg agcagctcag cggcccggtg tgggcgagca agggagatcc ccggatcacg
     3001 cgcctggggc agttcatgcg caagacgcgg ttggatgaaa tcccgcagct cctcaacgtc
     3061 ctcatggggg atatgtcgct ggtcgggccg cgcccggagc gtcccacgtt tgtggccgaa
     3121 ctctcctcga aaatcgacaa ctactgcgac cggctgcagg taaagccggg gttgaccgga
     3181 ctggcgcagg tcgaatgcgg gtacgacacc gacctcgcct cggtggtgaa gaaggtcagc
     3241 tacgacctgg cctatatcga gaactggtcg atctggctgg aaatcaagat tctgctacgt
     3301 acggtggtgg tggtggtgac gggccggggt gctaattagg cgccccctgt gaccaccgag
     3361 cctgagagac ctgtcaaacg gccgtgttcc acggccgttt gtgttatatc ttgtgccggc
     3421 tcaaacgaca cactattttc ccgataatca gagtaacccg ttcaccaggg aagaaggagc
     3481 ggaaggctga aagggattct ggcattgata ctggcggcgg cgctcgcgcc cgtcccggtc
     3541 gcggcgcagg cggacagcga tccgggagtc atcgtcagga cagacccgcc gggctgtctg
     3601 gtgacgctcc ggggagagat ccaggcaagc ggtgtcgcac ccgtccactt ccgccaactg
     3661 cttatcgggg actaccggtt gaaagtcgag cggccgggtt gggagaccta ttcgagccgg
     3721 ctggtgctcg acccgacgaa gcaggtggcg gtcgatgtca atctgtcgcg gaagacgcga
     3781 ttcaagggcg cggtgcgctc gctcgtcatt cccgggtggg ggcagcggta tgccgacaaa
     3841 gatctcaaag cgggagtgtc tttcgggatg gctttggcct ccggcgcagc ctttctgttg
     3901 gccgacgacg aattcgacta ccggcacggg gtctacaacg aggagaagcg gaagtacctg
     3961 gcgctgccga aagacgcgcc cgccgaggtc cagcaggcga tgttcaactc cgtgaagacc
     4021 gcccatgaga aagccgtcga tgcggagaat gtgcggcggg cgacgattgg cgcggcgatc
     4081 ggcgtgtggg cgctcagcct gctcgacgcg gtgctctttt tcccctcgga acaggggacg
     4141 ttttcgatca aaggggtggc ggtggcgccg gggcgggtaa gcgacggcta cggcgtcacg
     4201 ctgtccggcc ggttctagcg aggcggagcg aggcatggcg cccgacccgg ctcaacatca
     4261 acagcgaggg gtacacctga acgtggcaag atttatcggg cttctgattg tgctggccgt
     4321 gctcggcgcg gccggctgtg aacggtatct cgactcgacc gatcccggcg tggccgatcc
     4381 gggagggatg gcgacgcccc ggatcacctc ggcggccatg ctcgacgggg cggtgattct
     4441 cacctggcgg gtggacaacc cgcgggggat tgtccgctac cgcatttacg ccacggacgc
     4501 gaccgatacc gccggcggca gctacctgct gcgggattcg acgacggcga ccacggacac
     4561 catcaccaat ctcccggcga gccagtggta cctgttccgg gtggccgcgg tgcgctcgac
     4621 cgggtacgag gggccgcgct cggagccgtt cgcggcgagc gtgcagcacc tgtcgattgc
     4681 gatcaacgac gggagcgagt acacctcgaa ccggaccgtg caggtgcggg tcaacgcgcc
     4741 cgccggtttc gccacccaca tcatgatgtc ggaggcgccc ggtctgacgg acgcggcctt
     4801 taccccgctt ttcgggacgg cggcggcgtt cacgctgtca accggcgacg gggtgaagcg
     4861 cgtgtacgcc aagctgcagt ttgaggacgg gctggtgacc gcggccgccc tggtcgactc
     4921 gatcacgctg gacacccagg ccgagatcga ctcggtctat ttcggagatc ccggggctga
     4981 cggatatttc gggctggggg acgtggtcac gttcggcctg gcggcagccg gggagcgcgg
     5041 gggtgcggcg gccgtctcgt tcgccggggc gggaaccgtc acgttggccg atgacggcac
     5101 gggcggggac gacgccgcgg gcgacggccg gtaccatggg cggtggacgg tgccggcggg
     5161 gttcacgctg gcggccggcc tggtgaccgg gacgtttacg gatgccgcgg ggaaccgccg
     5221 ccaggcggcg gcgccgcgct ttctcacggt gtatacgccg ccggcgccgg tgacggcgac
     5281 cgcgctggcg accgggccga ccacggtggc gctcagttgg acgtcctcgt tgagctcgat
     5341 tttcgcggcc taccgggtct accggtcgac ctcctccgga gttgggaacg aggcgacgct
     5401 gctcaccacg atcacggctc aggcgaccac gacttacgac gacaccgggc tgaccccggg
     5461 aacgacgtac tactaccggg tggacacggt cgatcggtcc ggcctggcgg cgcgttcgct
     5521 cgtcgtttcg gcccgaccgc aatagcggcc ggcagggtgg cacaggcgat gggaattgtc
     5581 gacggcatca accggctcat cgggctgatc ctggacgtgt tccggcagat cgggcgcggc
     5641 cggatctggc tgttcctgct ggtgtatttc gccgtggcct ggtttttcct cctggcgctc
     5701 caccgcttca cctcgcctct gttctattcg gcggtgacgg cgtggaccac gctgatcgac
     5761 ccgcagcggg cgaccgggtt cacgcactac cgggggcact acctgctgct gccgtttttc
     5821 tttgactggg ccaagctgat catcagcatt ctgttcgagg gcgtcatttt cgggagcgcg
     5881 gccgtgctgt tctacgagcg tttcgttcag gtggacgaag aggaccgctt caggttccgc
     5941 acggtggccc ggttgtggat ccacctggtg ctcgccctgg tcattatgaa cggcgtcatc
     6001 ctggcggtca atgtcggcct ggccaaggtg ctccacccgc ttttggagta ttcgccacgg
     6061 cggcagtcgg cgttccgctg ggtggggctg ccggcggcct acgtgtgcgt tgccgggctg
     6121 ttcttctacc tcgtgccggc gatcgcggtg tacggcgaga atatcatcga cggcctgcgg
     6181 cggacggtgc gggtgttcct gcgcaacccg gtgacatcgg tggtcatggc cgggctggtg
     6241 ctggcggggc cgatcctcct gtcgaacatc gtcagttcac ccgacaccct ggtggaccgc
     6301 ttccggccgg aactggtcta ctacctgctg gtcgccgggc ttttcgcgga ggcggtggcc
     6361 ggattcttct ggatggggct gtcggtccga atgctcgtgg atcaggaaga gtagtttctc
     6421 ccggtcccgc ttttcttccc ccgttctccc ctcggaaaca ggcgctgttg catttcgaaa
     6481 ctgctgctgg ccgggccgcg cccgggtccg ctgcgcccgc tccgggggcc ggtttttgcc
     6541 tgtgccgcgc cgcttttcca ccttcaagaa tcagccggac ggccgataat acgagtgtct
     6601 cgtagaacgg tgatagcgcc aatgaaaaac atacttctgg tcgacgacga taaagaactc
     6661 tcccagtcgc tggccaacct gttcgacagc gacaaattcc gttttcggtt tctcgaggac
     6721 gggacggagg tgccccaatt cgtgacccgc cacgacgaca tcgacgtggt gatgctcgac
     6781 gtcaacctcc cgtccatgtc gggtctcgag gtgctcaagc agatcaagca ggtgcgggac
     6841 cagctccccg tgatcgtgat ctccgggttc gtatcgacgg agaatgcgat tgaagcgatg
     6901 cgggaggggg cgttcgagta tctgaccaag ccgttccaga tcgaaaagct gatcgacacg
     6961 gtgaccaaag cgtgcggcta ctcggcgccg gggaaacgcc cgccggcggc ggcgccggag
     7021 agcgcggtcg acgccatgca gggggaaatc gtcggacagt cgccggagat tgtcgagatc
     7081 gccaagctga tcgggcaggt ggcgaaaacc gacgcggcgg tgctgatatt cggggagtcg
     7141 ggcacgggca aggaactcgt ggcgcaggct attcaccgga actccaaccg ccgccacaac
     7201 gatttcctct cggtcaactg cgccgcgctg cacgagaccc tcctggagtc cgagctgttc
     7261 gggcacgaga agggggcctt caccggggcc tacttcaagc gcatcgggaa attcgaacag
     7321 tgcgccaagg ggacgctctt cctcgacgag atcggggaca tgtcgatgct cacccagtcg
     7381 aaactcctgc gcgtcctgca ggagaagcgg ttcgagcggg tgggcggcaa cgagacgatc
     7441 gaagccgacg tgcgcatcat tgcggcgaca aacaagtcgc tcgtccaggc gatgaaagag
     7501 gggtcgttcc gggtcgatct cttctaccgg ttgaaagtat tctcgatcta tctgccgccg
     7561 ctgcgcgacc ggcgctcgga cgtgccggtg ctgctcgacc acttcatcag aaagttctcc
     7621 aagcagctgc accggccccc caaaccggtg tcgaaacggg cgatgcagat gctcgtgaag
     7681 caccactggc cggggaacgt gcgggaactg gagaacaaca tccagacggc gctggtgatc
     7741 gccaaggggg acgagctcga gtgcgaagat ttcccgatct tcagcgagag caccgggcgc
     7801 gtggaagtca acctcaacga ccttcaggaa gactacaccg aaaccttccg gcggatcatc
     7861 gaaccggtga tgcctaagct gatcaacaat aatcccggcc aggtcttcca tttcctcgaa
     7921 tcggcgttcg agcgggcggt gatcgcctcg tgcctcaagg aattcgaggg gaaccaggtg
     7981 aaaacctccg aggccctggg gatctcgcgt aacacgctgc gggaccggat ctcgaggtac
     8041 ggaatttact aaaccccgca cgcggccaaa gcgattgcat ccggctgctc ggcccccggt
     8101 ccaggcagcc ggttttcttt ggacgggagc ccgggcgctt ccgccgagcg gccgtcggcg
     8161 ccgccagagg gcgcagttgc ccgtcagccg ctcctggcgt atattcccgg catgaggata
     8221 tgccatctgg gagacagcca cctcggggcg ggagccaacc acccgcgccg gggggccagc
     8281 gggttgacgc tccgccagga ggacatcatc gccgccttcg ccgaggcggt ggacaaggtg
     8341 atcgcgctgc gcccggatgt gtgtattcac gccggcgact tgttcgacgc ggtgcgaccg
     8401 ctgaacaggg tcatggcggc ggcgggggag ctcctgcacc ggctggcgga agaggcgggg
     8461 attcccacgg tcctgatcag cgggaaccac gatgcgccca aacagccgca cgtcggagcg
     8521 ccgatcgacg tataccggca gatcgacaac ctgtacatcg cgtcggcggg gcggagggag
     8581 gtgttccgga tcggggaggc cgcggtgcac gcgctcccgc actgcctgac cgggccgatc
     8641 cttcaggagg aactcgcgca gtgccggccg gaggcgtccg cgcggtacaa cgtgtttgtc
     8701 ggccacggag tcgtggcggg gatgccggag ttcgcgatgg cggatctcgg cgagcaggag
     8761 attgaggtcg ggaaactcga agggttcgac tatatcgcgc tcggccacta ccacaacaac
     8821 tgcgaggtgg gcaaacgggc gtggtactgc ggatcgacgg agcggttgtc gcaggcggaa
     8881 cgggaggcgg agaaggggtt tctggaagtc gtgctcgatc cgttcgaggt gcggttccac
     8941 cgcgtgcacg cccggccgat ggtggatatc ccgccgatcg acgccacggg aaaacggggg
     9001 gatcaactgg ccgagatgat ccgggcgcag gtcaccgcgg ccggggccga ggacaagatc
     9061 atccgggtgc gcgtcgaagg ggtgacggag gagacgctca agacgatgcc gagcgacgtg
     9121 ctgatggagc tgaagcagca atcatgggcg ctggatctga cgttccaaaa ggcgaaagca
     9181 gagggcgccg gggaggcgtt cgggcgggcg gcgatcggac ggctcgacca ggagtttctg
     9241 cggtttctgg agagcaccga gattcgcggt tttgatcggg atcggctcag ggagatgggg
     9301 ttggcgtacc tggcggagga ggagtaggag gccggcgcga ctccgggagg aggcgggaga
     9361 aaatccgggc cgcgccgcag aaatgcccgt ataagaatgg cggggatttt ggttgactct
     9421 cgcgccgggc ggcgataccc tccggcgagg ccgctgcggt tcgagcggcg gtgaatctga
     9481 caagacgaac atgaccacac gtcggagcta ctaaggaagg atagtgcgat gaagaaagta
     9541 ctggcagcgg cccttctggc ggccctcctt gcggccggag aggcgatggc gatttcagcg
     9601 caagggagcg ggttcgggac tctggccacg gcccggggga tcggggcggg acgagcctca
     9661 tttgccggcg gggtcgggct ggccgacgcc accaccgcct tcggaatggt ccgctacggg
     9721 ttcgccgact acctcgaagg gcggttcaag ctgggattgc gcgacgatgc cggaatggac
     9781 gcggtgctcc agatcggggc ggatgtgacc taccagttct ggtcggtggg gccgcagtcg
     9841 ccgaatccca tggacctggc gttcgggggc atgattgagt atttcccgcc ggattatctt
     9901 gacgacgtcg acgagagcct gtggatgttc ggcggttacg cgatcgggtc ctatcccttc
     9961 gcgctgaaca acgggatgaa gctgtcgccc tacggccgcc tcaatctgcg ggtggagagt
    10021 ttcggcaccg atgttcgcgg gggagacaat tccgagctgg agttcggcct gaacggcggc
    10081 gccatgcttg aaatcacgcc gactctcaac gcctacggcg agttccaaat cgacgggaac
    10141 gacggcgtgt tcctcggact ggagttcctc atcatgtaga gcgcggggcg aggccccggg
    10201 aactccacgc cggaactcac gtgacctatc tcgatgcact cattctcggt ctgatccagg
    10261 gtctgaccga gtttctcccg gtgtcatcct ctgggcacct cgtgctggcg gaggaaattc
    10321 tgggcgtcaa gcagcccggg gtggcgttcg aggtgctggt ccacctgggg acactggtgg
    10381 cggtgactat ctactaccgg cggcgcctgt gggccttggg gcgggcggtg ttcgaccgga
    10441 ccatgggctc ggaacgaaag atgatcctgt acctgattat cggcacaatt ccggcaggtc
    10501 tggccggcgt gctgctggag gattttttcg agcgcacatt cggcaacccg atcgagacct
    10561 cgatcgagct gatcatcacc ggggtcatcc tgctgatcac ccggtacttc cttcgcggca
    10621 agaagccggt ggcggcggga aacgcgctga tcatgggggt cgggcaggcg gtggcgattt
    10681 tcccgggcat ttcgcgctcg ggcacaacga ttgcggcggg gatctttcgc ggcgtcagtc
    10741 ccgcggcggc cgccgagttc tcctttctcc tctcgatccc ggctattgcc ggcgcaatcg
    10801 tcctgaaggt gggagagctg tccgatgtga gcgccgccct gctcgggccg tacgcggcgg
    10861 cgaccgtcac ctcgttcgtt ttcgggtggg cggcggtgtc gttcctgctg gctgtaatcc
    10921 gccgcggaaa actcgactac ttcgcctact actgctttgc ggccggggcg ctcggcctct
    10981 atattttcct ctgacatgga gaagatcctg gtcatccggt tcgggtcgct gggtgacgtc
    11041 atcctcacgt cggcggccgt gctgaacctc aaaatcagtt ttccgggcag ccgcctgaca
    11101 tttttcacga aagaacggtt cgggggcctg gcggctgctt tcgacggcgt ggatgaggtc
    11161 gtgtgtctgc cggagggagg ggacgggcgg gagctggtgc ggctggcgct cgaattggac
    11221 cgccgggggt ttgacgccgt cgcggacctg cacggcaatt tccgatcatg gctgacgcgc
    11281 aagatggtga cggcgccgca cgcgaccgtg tatccgaagc ggcggctcgc gcgacggcgg
    11341 ttgctgcggg ggcggaaccg tctggcgccc ggtccgcaca cgatcgacct gtacaaccag
    11401 gcggttcggg caatcggggg gaaaaccccg gcgcggcgcc cgctccttcc cccgccggtg
    11461 ccggacgcgg acctgccgcc ttcggtgcgc gagttcctcg ggcggcacca ggtgtgcgtg
    11521 gcggcagcgc cgggcgccgc ccaccgcccg aagcagtggc cggtggagcg atttgcggcg
    11581 gcggcggccg agctccaccg cacgtacggg gccggaattg tctggatcgc cacctccgcg
    11641 gaacagggga aaggaccgaa accgcccgcg gtcgcgcccg agcactgcgt ggagctagtg
    11701 gaccggccga tcggcgagat cgccgcggtc ctgcgccggt gcacggcgac gctggccaat
    11761 gattcgggga tcgcccatct ctcatcggcg gtcgggacgc cggcagccgt cgtcttcgga
    11821 ccgacccacc cagccctcgg cttcgcgccg cgcggactct gtgaccgggt gctcgaagtc
    11881 gaggaatact gccgcccgtg ctcccttcac ggcgaaaaag cgtgctttcg cgaggagccc
    11941 tactgcctca cgcggatttc gcccgaggcg gcggccgaag cggtcgcgga tctgatcgcg
    12001 cggcggcagg cggagcgccc ggccatcctg gccgatcgcg acggaacggt gatggccgac
    12061 aaggagtacc tcggcgatcc cgccggggtg gaactggtcc cgggggccgc ggagagtttg
    12121 cgggcggcgc aggcacgggg gtaccggatc gtgatcctct ccaatcagtc cggggtggcg
    12181 cgcgggctgt ttgcgatcga ggcggtggag cgggtcaacg cgcgcctccg cgacctgctc
    12241 gcccgggccg gcgtacgggc ggacgggatg tactactgcc ctcactacga agacggcgcc
    12301 gtgcgggagt atgcggccgc gtgcgggtgc cgcaaacccg cggcggggat ggcggaacag
    12361 gcggcccggg aactggggat cgatctgcgg cggtcgtcgg tgatcgggga caaagcggac
    12421 gatgtgaacc tggcgcgggt gatgggcggg cgcggggtgc tggtgcggac cgggcacggg
    12481 gcggcggaag aggcgaaaat ccgagcggcg gccggcgccg cggggatcgc agttgcggaa
    12541 gatctcgcgg ccgccgtaaa gcttgtgatg gacggtgatt gcgcgtgatc agggcggccg
    12601 catacagcga ggcgcttccc ggcggcggga tcgtgtgcac gctctgcccg gtgcgctgcc
    12661 ggttccgcga gggtcggcgg ggcgtgtgcg gaagccgctt catgcgcgac ggcgagctgc
    12721 tggtggacaa ctacggcgag gcggtcacgc tcgcgctcga tcccatcgag aaaaaaccac
    12781 tctaccattt tcatccgggg accgacatcc tctcgaccgg gccgaacagc tgcaacctgg
    12841 ggtgccggca ttgccagaac tgggcgattt cacaggagcg ggcggagacg aaattcgtgt
    12901 cgccggagag tctcgccgcg gcggcccgcc gctacggcgc attcggggtg gcgttcacct
    12961 acacggaacc gctcatgtgg ttcgagtaca ttcgggacgt ggcgcccatc ctccgacagg
    13021 ctggcctcgc agtcgtgctg gtgaccaacg gctacatcga ggcggcgccg ctggaggaac
    13081 tcattccgct ggtggacgca atgaatgtcg atctgaaggg gatggcgccg tcgttctatc
    13141 gaacggtctg caaggggcgg gtcgaaccgg tgctccacac gatccggcgc tcggccgagg
    13201 cgggggtgca tgtcgaggtc accaacctgg tgattccgac gctgaacgac tgcgaggagg
    13261 atatccgaaa gctgaccgcg tttgtggcct cggtgtcgga gcggataccc ctgcattttt
    13321 ccgcctatcg gcccgagtac aaaatgcggc tgccggcgac gccggcgggg acgctggtgc
    13381 gggcgcggga aattgcacag gagcggctgt ggtacgtcta tctcggaaat gtgagtgtcc
    13441 cggggggagg ggacacgctc tgccggggct gcgggaaaag tctggtggag cgccggggat
    13501 atcggacgga gatcatcggt atggacggga gccggtgttc atcgtgcggt tttgaggcgg
    13561 ggattgtccg ataatgacgg ttgtccgaag tgccgataca gattatagtt accaggcaac
    13621 cgaagcttcc tcgcggcctt agggcggtct ctttccgcaa ggcggggcga gtcggcgatg
    13681 ggtgaacggc ggatgagaaa ttcccggaaa atgaagtggg tgtggacggc cgtgctggcg
    13741 gccgcggctg tcggtctgtg gctgccggcg caggcccagt tctatttcgg gaaaaacaaa
    13801 gtccagtaca cccgtttcga ctggcaggtc atgacgaccg agcatttccg catctacttc
    13861 tattccgagg aggaggagat cgcccggatc gcggcccagt tggcggagga cagctaccgg
    13921 gcgctggcgg cgaaattcaa ccacgagatc aacgagaaga cgccgctgat catctactcc
    13981 tcgcccagtt atttcagcca gaccaacgtg atcccgagcc ttctgccgga gtcggtgggg
    14041 ggattcaccg agttcatgaa ggggcgggtg gtgctgccgt tccacgggtc gtactacgat
    14101 ttcgcgcacg tcctccgcca cgaactggtt cacgtgttcc agctctccaa gatggaggcg
    14161 gtgctcgcgc gcatggggcg ggtgaatttc gggcggccgc cgctgtggtt tacggaggga
    14221 ctggcggagt actggtcgaa ggcgtgggac gccgaggccg agatggtggt caaggacatg
    14281 gccgtcaacg aactccagct cggaatcgag gacctctacc gggtggcggg ctcttactac
    14341 atgtacaagc tcggcgagtc catctgcact ttcatcgatt cgacttacgg accggagaag
    14401 ctcactctgc tgtttgagaa tctcgccaag gggaagacgt tcgacgaggt ggtgaagctg
    14461 acgctcggtg acgaccttcg cgaggtctcc cggaagtggc ggtattcgct caagaagtgc
    14521 tactacccgc agttggagga ggcggggctg ccggatatgg agtcggtgca gatcacgcgc
    14581 gacggctatt cggtgcgggg cgtgccgatc atgtgggacg ccggcgacgg gcggcgggag
    14641 tggatcgtgt tcaaggccaa tcggatgggc tactcgggga tctacatgac cccctccggt
    14701 gaggggcgcg gccggcgggt aaagacgctc ctgaaagggg agcgctcgtc cgatttcgaa
    14761 tccctctacc ttctgcggtc ggggattgac gcgaccgact cggggatggt cgtgttctca
    14821 tcgaagtcga aagggaacga cgttatctac gtctacaatc tgcgcgagga gcgggtcacg
    14881 caccgctacg agttcgccga tctcgcggga gcgcattccc cccggctttc gcccgacggc
    14941 acgcagttgg tgttcgcggg ggtgacgctc ggcgggatgg ccgacgtcta cgcgctcaat
    15001 ctggcggacg gcgcctaccg cccgattctg gaggacatct actacgacac cgatccggtg
    15061 ttttcgcccg acgggaacca gatcgtgttt gcctccgacc gcgggccgga ggggaaagag
    15121 ggggcgctca acctgttctc ctacgccctc ggatcgggga ccgtgcggca gttgacgttc
    15181 gggcgttacc gcgaccagac gccggacggc gcggccggcg ggatctattt cacctcggac
    15241 cggaacggga cattcaacat ttaccggctc gacagcctcg gcggcctgac gcggcagtcg
    15301 acctacgtga cgggggcgtt tgaaccgcgg gtgacgccgg atggaaaggg gttggtgtac
    15361 agcgggtacc agaagatggg gtaccaggtg ttccgcatgg agctcccggc cgagccggcg
    15421 ccggtagcgc aggagacgcc gccgccgaca ccgtcctggc ggcccgatcg gatcgactcg
    15481 aaatacgtga agagctcggt gaagtacgag accgactact cgctcgatat tgcccagtcg
    15541 gcggtcggct acgacccggt ctacgggtcg ctcggaggtt tccagttcgc catgtcggac
    15601 atgctgggaa accactcgta ccatttcctc ctgaccaaca cggccaatac caaggacgag
    15661 tttctgcagt cgttcaatat cggagtgacc tacctcaacc gggagcggcg gctcaactgg
    15721 gggatcgggg cgttccacct gtacgacgag tatttcaacg actacgacgg tttctactgg
    15781 gagcggcagg cggggatgat cggggtgttc agctacccgt tctccaagtt ccaccggctg
    15841 gacgtgacga cggtggggcg gcattcgaac cgggaggagg ggacacggac gcgggaccgc
    15901 cgggcggtgt tgatgacgaa ctatctcagt tgggtgtacg acaactcgct gtgggacatt
    15961 tcggggccga tcgacgggcg gcggtacaat ctcactgtcg ggctgaccac gcggctggac
    16021 ggggtgcggt cgttcaatcg gctggcggcg gccgacgtcc ggcattattt ccggctcggg
    16081 cgggtctcgg cgttcgccaa ccggctgttc gcctactggt cggaggggac cgagccgcag
    16141 cggatctatt tcggggggag ctggtcattc cgcgggttcg accggcggga atggtacacg
    16201 cggcacgtgc tctttgcctc caacgaactg cggttccccc tgatcaacgt gctgagtatc
    16261 ggctcgccga tcggcggtat cggctttcgg gcgattcgcg gggcgatttt cgccgatgtc
    16321 gggtcggcct gggaggaaga gttcgaccag ttctacggat cgttcggggc aggattccgg
    16381 gtggccttgg cgaacctgat cctcctgcgg tttgatttca gccggacgac ggatttcgag
    16441 acgatcagcc gcaacaccga gttcgacttc ttttttggat ggaatttctg atggcgcccc
    16501 gtctcctgac catattggcg gcggccgcgg ccatgctcgc ggcggcgtgc tcgtcgagcc
    16561 tgcagctctc cccggagaac cttcaggagg gatccccctg gccgtactac cacggcggcg
    16621 cgagcgccac cgggtgcgca ccggcgggcg agtttgcggg ccgcctggac gtgatctggg
    16681 aaacctcgat ggcggggaag ccggccggtc cgctcacgct ccacggagga tcgctggcgg
    16741 tcccctcgac gcgcaaccgc gtggagatgt acgagaccgg gacaggccgg cgatccggca
    16801 agatcaagac gaagggaccg gtgcagtcgg ggctggtggc ggaaggggga tacgggtact
    16861 gcgcggtcag tatcgggaat cggctgagct gtttcgactt gcggtcgggt gacgagcaat
    16921 ggaagcggtt tgtcaaggaa accgcgcggg gggccattgt cgccgctaac cggcttgtga
    16981 taggctcggc gacggggcgg ctcacggcgt acgatccggc cgacgggcgc gaagtgtgga
    17041 cgtttgagga aacacaggac cggtttatgg ccccgccggc ggcgggacac gggttggtgt
    17101 tccagccggg ggtcagcggg tttctttacg ccctggaccc gaacgacggg acggagcgct
    17161 atcgcgtgaa agtggacggg ccgatggtcg gcggcgtggc ggtcgccgaa ctggtcgtgg
    17221 gggcggacat gccggggcgg gtctacggga tcgacgctca ggacggtacc gtccggtggc
    17281 gcaccgacat aggagcgccg gtctggacca agccggcggt ggccgaggga cgcgtttttg
    17341 tcgggcattc cggcggcgaa ctggtggccc tgaatctggt cgacggccgc gtgctgtggc
    17401 gtttccaggc gttcgaggtg atcgcggcat cggcggcggt gatcgggcgg ttcgtggcgg
    17461 tcgggacgct gggcggcaac gtgtacgttc tcgacgcggc cagcgggtcg gtgctgagcc
    17521 ggcgcaagct ggagggacgt attgaccagg cgccggcgac cgacggcgac cgactctatc
    17581 tcgctaccga ccgggggcgg gtgatctgtt tgggcaccct cccgggggtc gccgcggagc
    17641 aagcggaagc gcaggccgac gatggaacag agtctccgga ggccggggcg agcggggcgc
    17701 atagggataa ccccggactt acgccgcccg ccgggtggac agccgccggc ggcaaggctg
    17761 gccgggcagg aggggggccg ccgggcagta aagctgtcaa gaggtgaacc caaaaagaga
    17821 aagggacagc ttggcagctg tcccttggat ccatcaatcc tcggccggag cgcgtcagcc
    17881 ctcttgcacc tgctcctcgg ccgctttctt cagtttgtca ttggcgtaga tggcgatctc
    17941 tacgcgccgg ttggcctgca tggcggccgc ggtgttctcc gtggcgatcg gctgctgttc
    18001 gccgtaaccc tggatgatga agcggttgct cactacctgc tgggcagcga ggaaactcga
    18061 gaccgactcc gcgcgggagc gggagagcgt gaggttgtag gaatccgatc cggtcgtgtc
    18121 ggtgtgcccc tcgatgagga cattggtctc ggcgtacttg ttgagaatgg cggcgaggtt
    18181 cacgaggttc tgctgggcct cgggccgcag gtcggccttg ttcacgtcga aaaggattcc
    18241 ggaagcgaac gtaatcttga tgccttcgcc gacacgctcg atccgcgccc cttcaaggtc
    18301 cttctgcatt tcctcagctt gctgatccat gtagtggccg atataggcgc cggccgcgcc
    18361 gccaacggcg gcgccgatga tcgcgccgac cgccgtgttg ccggcggcgt tgccgactag
    18421 tccgccgacc acggcccccg acccggcgcc gatcagagcc cctttctgcg ttttgctcat
    18481 accgcagccg acaagggcgg cgatcagcac cacgcaaatc ccggtcaaag caatgtgcct
    18541 aagcatgagt cgctactcct tgaacaattg ggttatgcca tatgccaagc tacccgtact
    18601 acacatcggg agattcgcat gcgaatccaa ctaaaggatg gcgaattccg gggtggcgtc
    18661 caagtttttt ttcggactcc ggactaacca gcttgactgc cgggaggctg actgccggat
    18721 tggcggttga gctggccagt tgacagacta tcaccgccta tttggcagcc atgctgacat
    18781 tcttggcagg ttggtcatcg gaacgagcag attcgttata gtgatattgt tgtcaatcat
    18841 gtgcttatgt ggaatggcac ggaagttgat aatcacttat gacagaagtc acagaaggaa
    18901 tcgagaaaca gagagacgaa ataaggaggt tccagaaatg cgtagcgtga tgattccgaa
    18961 tcggttggcg cgggaaatcg acagcatgtt caacaccttc atcagttttc cgcagatcag
    19021 tgccgagtgt gcgtgcgatt ttatgccccg cgtgaatatc cgcgagacca aggatgatgt
    19081 gcgcctggtg ttcgagctcc cagggatcga caagcaggac atcaaggtca cggtgaaaga
    19141 aaacgtgctc acggtgtccg gagagcgcaa gacggtggac gacgagaacg cggaggtcgt
    19201 tcgccgggag atcctcaccg gggcgttcag ccgatcgttc acggttcccg acacgatcga
    19261 caccgacaag atcgcggccg actaccggaa cggaatgctc gaggtgaagc tggccaagaa
    19321 ggaagcggtg aagcccaagg agatcgaagt cagcgtgggc tgattcacca gtccagaccg
    19381 gaaagccacg ccattgccga ccgacgcgat ggagatttcc gaaggaatcg agaggccggt
    19441 ccatgaccgg ccttttgtta agaagacagg agtgcggacc gggccggggg gctacgaaga
    19501 caggaggatt tgacggatga gcaagataat cggaatagac ctggggacga ccaactcggt
    19561 ggttgcggtg gtcgaggggg gcgagccgac ggtaatcgcc aacagcgagg gagggcgcac
    19621 cacgccctcg gtggtggcga tcgatcggaa cggcgagcgg ctggtgggga cggtggccaa
    19681 gcgccaggcg gtcaccaacc cgctgaacac tgtgttttcg gtcaaacgcc tgatcgggcg
    19741 ccggtttgac gaagtgcagg cggaaatcgc gaattttccc tacaaagtca accgcggacc
    19801 ggacgaatcg ccggtgattg agatggccgg ccagacgtac aagccggccc agatctcggc
    19861 catggtgctc gggaagatga agaagacggc ggaggactac ctcggccagg cggtgactaa
    19921 ggctgtgatt acggtccccg cctatttcaa cgactcgcag cgccaggcga ccaaagacgc
    19981 gggccggatc gccgggctgg aggtcctgcg gattatcaac gagccgacgg cggcggcgct
    20041 cgcctacggc ctggacaaga agaaagtcgg caaagtggcg gtgttcgatc tcggcggcgg
    20101 cacgttcgac atctcgatcc tcgaactgag cgacggggtg ttcgaagtgc tttcgaccaa
    20161 cggcgacacc cacctcggcg gcgacgattt cgacaaacgc atcatggact ggctgatcga
    20221 agagttccgc atgtcggacg ggatcgacct ggggaaggac ccgatggcgc tccagcggct
    20281 gcgcgaggcg gccgagaagg ccaaagtcga actgtcgtcg acgctccaga cagccatcaa
    20341 cctgccgttc atcacggccg accagaacgg gccgcggcac ctgaacgtga ccctcacgcg
    20401 ggccaggctg gagtcgctgg tggacgacct gctcgaacgg acgaagcggc cggtgcagaa
    20461 cgcgctggcc gacgccaagc tcacggtcga ccggatcgac gacgtgatcc tggtcggcgg
    20521 gatgacgcgg atgccgaagg tgcagcagat cgtgaaagag ttcttccgca aggaacccaa
    20581 ccggtcggtc aatccggacg aagtggtggc gatcggcgcg gcgatccagg ggggcgtgct
    20641 cgcgggagac gtcaaggacg tgctgctgct ggacgtgacg ccgctgtcgc tggggatcga
    20701 gacgctcggc ggggtgatga cccggctgat cgagcgcaac acgacgatcc cgacgcgcaa
    20761 gtcgcaggtt ttctcgacgg ccgcggacaa ccagccgcag gtggatatcc acgtgctcca
    20821 gggggagcgc gagatggcga tcgacaacaa gacgatcggg cggttcattc tcgacgggat
    20881 cccgccggcc ccgcgcggtg tcccgcagat cgaggtgacg ttcgacatcg acgccaacgg
    20941 gatcatcaac gtgtcggcca aggacacggc gaccggccgg gcgcagaatg tccggatcga
    21001 agcctcctcg ggcctgagcg aacaggagat cgcgcggatg gtggcggacg cccagcagca
    21061 cgaggcggaa gaccgggaga agaagcgccg ggtcgaggcg cgcaaccggg ccgacgcggc
    21121 cgtctggcag tcggacacgc agatgaagga gctcggcgac aaactcagcc aggaacagac
    21181 ggcggctctc ggggcggcga ccgagcgggt gcgtgcggcc ctcaagggag acaacgccga
    21241 cgagatgagc cgggccgccg aggatcttca gaccgcctgg cagcaggtct cggccgacct
    21301 ctaccggcag gcggccgggc cgacagcggg cgctcaaccc ggcggcaacg gatccggccc
    21361 gcagaccggc gccgccggtc gtccggacgc ggacgcagtc gacgccgatt tcgaagaggt
    21421 gaagtgagga agcggcggat cgcgaggcaa ggcggcggtc ggtcggcgat ttccggccgg
    21481 ccgccggcgc atggtgaacg ccagcgggtc gccgtgataa tcgagaaatg aggagcgcgt
    21541 ggacagttta tcgttaggtg tgatcggcgg tctgctggcg gggatctgtc tcttcgccgg
    21601 ctgggtgatc aaacggcact ttgtggacca acgctgggcg gcgatgggcc ggagcgttta
    21661 tgctgcgtgg cagaacggcg acgagcggca ccggatcgaa tacgtcaatt atctcgagga
    21721 ggatgcgagg gaggaggaca aggagggaga gtttcggccc gggcggcaag tgtgagagcc
    21781 ggcacctccc gggcgcgggc gagaagtatg cttgccgaag cagtattagt tacgatagat
    21841 tagacaaaat cggggggcga aagtcggcat ggcgaaggat tactacgaca tactcggcgt
    21901 gaaggaggac gcctcgccgg aggagatcaa gaaagcgttc cgcaagctgg cgatgaagta
    21961 ccacccggac cgcaatccgg gaaacgccca ggcggaggcg agattcaagg agatctccga
    22021 ggccaacgag acgctgtccg acccgaagaa gcgggcggag tacgacacgc tccgcaagta
    22081 cggggccttc gccggggcgg ggcagggatt cgacccgggc gcgcacggca acggcggatt
    22141 cgatttctcc gatctcttcc gccagggagc gggcggccgg ggcggatttc agacgttccg
    22201 attcggcggc ggggtggagg ggctggacga gattctatcg cagtttttcg gcgggcgggg
    22261 cgggttcgga acggagttcg gcaaagaacg ggtgcgcccg cgccgggccc ggcaggcgcg
    22321 ggggacggat ctgaaggcga cggtgacggt cacgctgcga gaggcggtcg cgggaacccg
    22381 caagcgcctc cgtatccgtc agaccggcag gacgatccag gtgacgatcc cgcctggaat
    22441 agaaaatggc ggcgtgatcc ggctggcggg gcaggggatg cccgcgcccg cgaggcccga
    22501 aatgccgccg ggtgtgcccg gagacttact catcacggtg aaggttatgg ccgaccatca
    22561 gttcgaaaga aaggggaatg acatctacac gaaggtcaca attcccctga aggatgcgat
    22621 tctcggcggc aaggcccggg tcaggacgcc gctaaaggag attcaggtga cgatcccgcc
    22681 gggaacgcag cccgggacaa aactccggct gaaggggatg gggctctcgg tgggcggcgt
    22741 gcagggagac cagtatgtcg aagtggatgt gcggatccca agagcggccg atctgacgga
    22801 ggagcagagg aagatgttgg aggagtggtg agggggcgca aatctcgaga gaatgttacg
    22861 acccatagca taggagcaac cgtagcatgt atgtcgtact agataccaat atcctaatgg
    22921 cggactacct ccttcggggc cggtggttca ctgacctgcg ccattttctc aggttgaata
    22981 acggcaagct agtaattccg agaatcgtcc tggaagagac tgtggccaat tatggtcgcg
    23041 actataaggc catcatctca gaggcgaaaa ggctagataa aaggctgaag agtctctcaa
    23101 agcgaaactc attggattat gagcgcttag agcaagaaag caaccgggaa atgaataatt
    23161 accgggagtt cttgttgagg gagttggaac actggaatct ggacgcggct tccgagaagc
    23221 caccaggggt ggatgacatc ataacagcgg tcgtcagtag aagaaagccc tttaaggaga
    23281 atggcgaagg gataggggac tacctgctgt ggcgtgcagt gactcaggtg gcgacaaatt
    23341 cagaaccgac tgtgctaata acaaacgaca ctagcgactt tgggaatcag atggatccgg
    23401 tgggtctgca cgatgccctg attcaggaac tgggggaaaa tgcgcccaag gtcagtctct
    23461 accggagcat tcagtcattt attgagattc actatccaaa gccaccgtgg gcaacggagg
    23521 agtacttgag gtgtctgcct ggatttcagc gagtagtgag cttggtgaac gcctttgtaa
    23581 aacgtatgcc cggtgaattc gtgtttcccg ctggcttgcg cctgataccg tccgaaccag
    23641 cggtgaaatc tttctacgtc taccagagcg gcgacgacgc atggacgacc gtacttcgat
    23701 tcgaagtcga agccagtgtt gagggtttgg ctccgagcag agtagcaaag ccggttgaag
    23761 gattagtccc attagcggcc ttttccggga atgtcactgt attggcctcg gtccactgcg
    23821 aggttaggaa tggagatata cgccttgtga gcacgttaga gcgggaagtc acaagagagg
    23881 atttgcgaga gggtacacta cggtatatcc gccggagcac agcaatgtat agtgaggcag
    23941 cgtccggcgg ggttcttctc tccgggatat acacggctcc agacttccgg cagtatcccc
    24001 tgccgtacgg cgccgttcac gctgaccctg tgcgtccaat gccaccgatc aaacccccta
    24061 gcgccccggc agtgttgtgg aagcgctccc aaccctcgga cggcgatgcc aagaaaggtg
    24121 actgacaagg tcgaggtttg atatgacgga ggaaggtgac acatagatga acccgaatcg
    24181 aatgacccag cggtcgatcg acgcgatcag ctacgcccag cagcaggcgg cggcggaaca
    24241 acacccgcaa atcacccccg aacacctgct ccacgcgctg ctggtccagg aggaaggact
    24301 cgttccgcag cttctgaaaa agacgggcgc cgagacaggg gccatcgctg gcgaggtcga
    24361 cgaggcgctc aagatgctcc cgcacgccgc gggcggccag atctatccct cgcgcgagtt
    24421 gagccaggtg ttcacccggg ccgaggcgga gctcgcccgt ttcaaggatg agtacgtgtc
    24481 ggtcgaacac ctgctcctgg cgctcctcga ggtcaggtcc aaagcgcagg aaatcctcaa
    24541 gggccacggg gtcaggcggg acgaactgct caaagcgctc tcgtcgatcc gcggaagcta
    24601 ccgggtgacg gacgagaccc cggaggcgaa gtacggggtg ctcgagaagt atgcgcggga
    24661 tctcacacag gcggcgcggg aggggaagct cgatccggtg atcgggcggg aggaggagat
    24721 ccggcgcgtc atcaagatcc tatcgcgccg gaccaagaac aacccggtgc tgatcggcga
    24781 gccgggcacg gggaagacgg cggtagtgga ggggttggcg cagaagatcg ccctcggcga
    24841 ggtgccggag acgctcaagg agcgccggct ggtggcgctc gatatcggcg ccctgatcgc
    24901 cggttcgaaa ttccgcggcg agttcgagga gcggctcaag gcgatcctca aagaggtcga
    24961 ggaggcggag ggggaggtcg tgctgtttat cgacgagatg cacacgctgg tcggcgcggg
    25021 ggcggccgga ccgggcgcgc tcgacgcctc caacatgctc aaaccggcgc tcgcccgcgg
    25081 ggagatccgg tttgtcgggg cgacgacgct caaggagtac cgccaacacg tggagaagga
    25141 cgccgccctc gagcggcgct tcgcgccggt gctgatcgag ccgccgacgg tcgaggagac
    25201 ggtgtcgatc ctccgggggc tgcgcgagcg ctacgaggcc ttccacggga tcaagatttc
    25261 ggatggggcg atcgtggccg cggccaagct gtcggaccgc tacatcgccg accggttcct
    25321 cccggacaag gcgatcgatt tgatcgatga ggcggcggcc gaactgcgga tcgcgatcga
    25381 ctcgctgccc gaggagctcg atgcggtcga gaagaagatc cggcagctcg agatcgaaaa
    25441 ggagggggtg aagaacgagc cggacgcccg cgagcggctg aaaccgatcg aggaggtact
    25501 gaaagacctg tacgcgcagc ggaacgatct ccggacgcaa tggacgcggg agaaggacac
    25561 ggtcgagggg atccgcgcgg tcaaagtcga aatcgaacag ctcaagcacc gggcggcgga
    25621 ggccgagcgc aaggcggact acgagacggc ggcgaaaatc aagtacggcc agattgtcga
    25681 ggccgagaag aaactccagt cgctgacggg cgccctggcc gctatccagg agaaacgcca
    25741 gctgctcaag gaggcggtgg atgaggacga gatcgccgag atcgtcgcgc gctggacgca
    25801 tatcccggtg gcccgcctga ccgagtcgga gtcggaaaaa ctgctgcgcc tggaggagga
    25861 actgcaccgc cgggtggtgg ggcaggacga ggcggtcgcg gcggtggccg acgccgtgcg
    25921 ccagtcgcgg gcgggactgt cggacccgca caagccgatc ggctcgttca tcttcctcgg
    25981 ccccaccggg gtcggcaaga ccgaactggc ccgggcgctc gcggagttcc tctacggcac
    26041 cgaggaggcg atcgtgcggc tcgacatgtc cgagtacatg gagaaacact cgatgtcgcg
    26101 gctcgtgggg gcgccgccgg ggtatgtcgg gtacgaggaa gggggccagt tgacggaggc
    26161 gcttcggcgg cggccctacg cggtcgtgct gcttgatgaa atcgagaagg cgcacccgga
    26221 ggtgttcaat attctcctgc aggtgctcga cgacggccgt ctgaccgaca accaggggcg
    26281 gacggtgtcg ttcaagaacg cgatcctggt gatgacctcg aatgtcgcgg ccgagtacat
    26341 tcagacggag acggccggga tgggggaatt caaccgggat atgatctggg agaatatcaa
    26401 gaagaacgtg ctcgacgcgg tgcggcggac gctgcggccg gaattcgtca accggatcga
    26461 cgaaatgatc gtcttccatg cgctcacccc ggcggagatc agagcgattg tccgcctgca
    26521 gttgcggcgg ctcgagcggc tcctggcgga aaaggatatc gggctgagcg tgaccgaggc
    26581 cgcggtgggt caaatcgccg aagccggtta tgacccggct ttcggggcgc ggccgctcaa
    26641 gcgctacatt gcgaaagaac tcgcgcagga tatcgccaaa cagatgctgg cggggacggt
    26701 ccggcccgga cagacgcttc ggatcgattt cgccggcggg gagatgcggt tcacggctgg
    26761 gccgacggca gaagggtcag ggccgcaagg gtcctgacgg ggctactcac accgttggta
    26821 agaatgtcag gaccacaagg gtcctgaccc gcgtcggcga tttcgcgtga tgaggcggcc
    26881 cggcgaaagc gccgctatgg ttggaccggt ttgccccgcc gggccgtgaa tccgaaactc
    26941 agggcgttgt attcacaggc tttgatgcat tcgccgcacg aggtgcagtc ggcgaggacc
    27001 ttgactttgc cctcgacgat ctgtttgatc gtggggcatg gggaggccgc cacgcaatcc
    27061 atgcactcgg tgcaggcctc gcggtcgatg cggacgcggg ccggcgcgat cttctcacac
    27121 agccaggtca gagcgccgat cgggcaaatg ccgtagcaga acggacggta gagcacgagg
    27181 ccggccagga gcaagacagc catcatgacg atgaacgtcg catcgacact ccagtggagc
    27241 agctcgaaga agttgatcgg atcgtagacg ctgtaggccg agaaggcggt ccaggggccg
    27301 acgctcgtgt ccgcctcgag ccgctcggcg agcagcccga catggttctt gacggcgaac
    27361 acggtcagga tgaagaggac aaagaggctc aggcggacgg cgttgaaggc ggtgaagttg
    27421 aactgcttga atttccactt gaagggaatt ttgttggcga gctcctgcaa cgcgccgagc
    27481 gggcacaccc agccgcaaaa gagtttgcgg ccgaccagac tgggaacgaa catggccagg
    27541 gcgagggcta caaagatggg aaagaacatg ccggtcgtga tcctgaacat gatcagattc
    27601 accaccccgc acatcggact tggatggagg gggaacaggt agtcaaggcc aaacaggacg
    27661 aacaccgccg ccatagcgcc gattcgcacc cacttgttga ccaggcggtt catcacgagg
    27721 atcatgccga tgacggcgat caccacgaag agcatgtagt gccaggtgaa gaggaaatcc
    27781 cagacggtcg gttcgacctc ctccacgtgt tcttccaccg cccgcagggg agcggcgcag
    27841 aggacggaga agagcgcggt gagcggcaac agacgacggt gcatgtgcgg cctttcggag
    27901 agcgacggtt tgtttgcggt agtatagggc gagggggacg agatgtcaat tgccgggctt
    27961 atcgaaggcg gaggggggag gcatggtcca cggaccgtcg cccgccgctg ttttccgatg
    28021 tcgctccggg gcggccgcgc cccggcgcgc cgttccctac ccctgtccga attcgatttt
    28081 cgaggggtcg ccgcggcgaa gggatttgaa ccgttcgaga atcagctcaa acacggcctc
    28141 ggccttgaaa cgcggctggc gtgggctgat gaccagttcg agaatgatga tctcggcccc
    28201 gccgttgtag taggagacca tcacggtcgg gacgaaggcg gcgggatcgt tgacgtagac
    28261 ggtggggatg atttcgccct gggcgttgca catggagtcg accggccaca tctcgatccg
    28321 cccgccgtag tggctgcgga tggcggccgt atcctcggcg atgatgccgg cgaaggaggt
    28381 ggcggcggag tcgaccttgt agatggtgat gccgacgaga aggtcggccg tgtcaaaatc
    28441 ctggtcgccc gggatgtagg ccatcgacag cccggccgcg gccgcttcct caagaacgag
    28501 gcggtagccg gcgggggggc tgaagacata cgttcgggcg gcgccttcgt agaaggcggt
    28561 gttgctgtcg aggcccggct cgatcaggtc ggcgaacgga ttggcggtcg ccgctgagag
    28621 cagggcggaa aagaccaaaa tcgggagcat agggctagta tcggcggggg cggcggtcgg
    28681 ggtgagccaa agattcgcgg cggatgcggc gccgagagcg gggcgggatg gccgggggga
    28741 ccgcgggaac gcgaaaatgt cggaaattgc gctgcccccg ggtcggcggg cttgtgttgc
    28801 cgccgccaaa tgcgtatgtt cggcccaatg gacaggacag caaaagacgt tgtttcgcaa
    28861 cggctaacca atacagaacg aggttgaggt atggcaggta tcgttggcta cggctgccat
    28921 ctgccccggc accggatcaa agtcgaagag atcgccaagg tgtggggggc ggacgcgccc
    28981 agctataaga agggcctgat gctgcgggaa aaatcggttc cgccgcccga catggacacc
    29041 attacgctgt cggtggaggc ggcccggcgc gccctcattc gggcgcagac ggtggacccg
    29101 aaagatatcg gggcgatcta catcggctcg gaatcgcacc cctacgcggt gaagccctcg
    29161 ggaacgacgg tgggggaggc gatcggggcg acgccggatc tgcactgcgc cgattttgag
    29221 ttcgcctgca aagcggggtc ggaggcgatg tttgtcgcgc tcagccacgt ggagtcgggg
    29281 ttcatgaagt acgcgctggc gatcgcggcc gacacctcac agggcgctcc ctcggacgcg
    29341 ctcgaattct cggccgcggc cggggccggg gcgttcatca tgggcaagga caacctcatc
    29401 gccgaatgtc tctacaccca ttcctacatg accgacatgc ccgatttctg gcgccgcgag
    29461 cacgagttct acccgcagca cggcggccgg tttaccggcg aagaggccta tttcgcgcac
    29521 acgaaaggcg ctaccaccgc catcctgaag aaatcgggga tgaagccgca ggatttccag
    29581 tgggcggtgt tccaccagcc gaacgggaag ttcccgcagc gggtggcgct ggaactgggg
    29641 ttcacccagg cgcagatcga tccgggctgg ctgtcccccc gcttggggaa cacctactcg
    29701 ggagcctcgc cgatcggcct gacggcgacg ctcgacacgg ccaaaccggg ggacctgatc
    29761 atgatgtgtt catacggttc gggcgcgggc tcggatgcgt tcatctggaa ggtcacggac
    29821 cggatcgaca aggtgcggga cctgacaatc aggacgcaca agctgctcga tgaaaacctc
    29881 acctatgtcg actacggcac ctatgccaag ttccggcgca aaatccgcaa gaacgcgtag
    29941 gaggagaaag aatgagaacg gtgtgtatcg ccggggtcgg catgagccag tggggcgaag
    30001 tgtggcgcac ctcgctgcgc cagctctttg ccgacgcggc cggtgaagcg ataaagaacg
    30061 cgggcgtcga ccatctcgac tccctctacg tggggtgcat gtcgggcggg ttgtttgtcc
    30121 agcaggagca tctcgggtcg ctcatggcgg actacatggg aatgccgggg ctgccggcga
    30181 tgcgggtcga gagcgcctgc gcctcggggg gcatggcgct ccgggcggcc tatttcgagg
    30241 tggccgcggg ggcggccgag attgtgatgg ccggcggagc cgagaaaatg accgactgca
    30301 ccggcgacca ggcgaccgcg gcgctggcca ccgcggccga ccaggagtac gaagtgttca
    30361 acggggccac cttccccggg ctctacgcca tgatggcgca cgcgcacatg gccaggtacg
    30421 ggaccacgcg ggcgatgctg acggccgtgg cggtaaagaa ccaccgcaac gggtcgatga
    30481 atccattcgc ccagtacccg tttccggtga aaccggagac ggtcgagagc tcggtcatgg
    30541 tggccgaccc gctgcgcatt ctcgactgct cgccgatcac cgacggggcg gcagcggcga
    30601 ttctgtgcag cgaggaagtc gccaaaaagc tcggacgccc gtacgtgcgg atcatcgggt
    30661 ccggcgcggc gaccgacacg atccagttgg cccagcgggc cgacctcacg acgatcaaag
    30721 cggccgccct ggcgacggcg cgggcgctcc agcaggccgg gcggaagatc accgacgtcc
    30781 agttcgccga ggtccacgac tgtttcacga tcgcggaact cgtggtgctc gagtcgatgg
    30841 gcgtgtacaa gccgggcggt gccggtccgg ccaccctggc cggggagacg gcgctggacg
    30901 gcaagttccc ggtcaacccc tcgggcggcc tgaagtcgaa ggggcacccg gtcggagcca
    30961 cagggatcgc ccaggcggtc gaggtgttca aacagctcac gcgccaggcc gaaggcggcc
    31021 gccagatcaa ggggaacctc cgcattggga tggcgcagaa catgggcggg tccggcggtt
    31081 cctcggtcgt tcacatcatg gaggtggcgt gatggctatg ttcagttcgc gcaattggcg
    31141 cgagtacccg cagcggtacc gcctcgaggc cgtgagattc aaaaagagcg gcaagaccta
    31201 tttcccgccg cgctcaatcg accccgaaaa ccgcgacacc gagcgcgaga cggtgcggct
    31261 ccccgacacc gggaagatcg tcacctacac ggtggtgcgg gtggctccct cgcagtgggg
    31321 ggacatggcc ccctatgccc tggcgatcgt gcaactgacc gacggcaccc ggctgttcgg
    31381 gcagatgacc gactgcaacg tggacgaggt gaaaatcgga atggaagtgc ggatcgagtt
    31441 ccgccggatc cagaccgagg aacaccacgg ggtgctgagc tacgggtaca agttcgtgcc
    31501 gaagtggtgg tgaggccgtt cgggaattga caccaggcgc cgccgggcgg aaacgcccgg
    31561 cggtttcttt tgcggctcac gaggacagaa ggcgcccggc cgtcaacttc gattgcgccg
    31621 ccgcccgcgc cgcggtatat tccccgtcgg cttcaggaca gcgcccgacc acgaaacgcc
    31681 tctagcgaga gcaggaaagc gaggaatcag atgtacatgc tgggagcagt cgccggggac
    31741 atcgcccggg agatcgcgca gatcaagcag gaggccgccg gccgggggat ccgggtgatg
    31801 cgcggccgcc tgatcggacc cggcggggcg gaggcgcccc gcgtcgtctg ggtggcggac
    31861 ggagacggcg actggcggga gtttctcgat ttcgcgcaga gtgtgggcgt gaaagcggtc
    31921 gtgatcgatt tcgaccggat cgacatggag ctcttgcggg cggatgtcga ggacgcggcg
    31981 gtcgacgagg agtacgaaga ggggcgcacg aagatgctcc agatgctgga gaaactcgac
    32041 aagcacgacg gggagatcgg gcatgtcggg atcatattcc cgcacggacc gctggtctat
    32101 caggcctcgg tgcggacgcc gtggtacgat gactacgtga cagtgcggga gatcgccggc
    32161 gccgcggatg agttcgcgga ggaagaggaa gaccctttcg atatccagtg acgtgcgggc
    32221 gggggctccg gccgctctgc ttcgcgccgc ggcccgaagg gcgttgacag aacgaggagg
    32281 ggatagtata ctattgaatg cgccccggca gctcgcgatt ggtgaatctc gggattgcag
    32341 tgaagcggcg attagtcccg gccccttggg cgcagggtac acagggattg acagcggaca
    32401 gacatatatc attatatcct atcttatccg cgggtcggga gcgtttggcc gagggcggct
    32461 cgttcgttcc cggcgcgcgc cgcaggctga ttaaccgata ccggagagcg gcgacgcagc
    32521 gggtgaaacg cgcaggaggt cttcatggtc cgaagtgtgg cgaggtttgt ggcgccggca
    32581 gcggtgcttg tgagtgcgct ggcggcatgg ggcggatcgg tccaggcgga cgagggaatc
    32641 gcgtaccacg atctccgcga ccgtgcgatc agcgcctggc aggaagtgga cacgttcacg
    32701 tttgccggtc aggtcggcga tgtcgtgctc attcgcgtga accgaacggc aggggaactc
    32761 aacccgctcg tccggctgta ccgtccggac ggcagccttc tggcggaggc cggaccgctg
    32821 tggtcggcct acgaccggac ggagatactc gatcaggccc tgccgcaagg ggggagctac
    32881 cgcctctgtg tggccgatga tgacggtctg cagtggggag actacgcggt gacgctggag
    32941 tgtgtgaatc ggccgaccgg ggcggagact gcggcgtatg acgatgtgcg gtccgacacg
    33001 ctcacagccg ttcccgagat aaaactctac cgcttctccg ggctggccgg ggaccgcgta
    33061 atcctgcgcg cgaatcggac cgcgggagaa ctgaacccgc acctgcggct gtaccgtccg
    33121 gacggaagcc ttcaggcgac ggccgacccg atgtggagtg cgtacgagcg cacggagctg
    33181 ttcgatcagg ggttgacgca gaacgggaca tacacggtgt gcgtgatcga cgacgacggg
    33241 ttccagctcg gcgaactggc cttcacgttg cagtgcgtga accggcccgt cggggcggag
    33301 gatatggtct accacggcct ggtcagtgcg acgatttcgg cggtgcccga aatggagctg
    33361 tatcgattct ccggccagat cggcgaccgg gtggtcctgc gggtgaatcg gtcttcgggg
    33421 gaactcaacc cgcacctgcg tttgtaccgc ccggacggcg gtctggtggc cgaggccggc
    33481 cccgtgtggc cgggcaacga ccggaccgag ctgttcgacc aaagcctcct gcaaacgggg
    33541 cagtacaccg tgtacgtgag cgacgatgac ggctcccagc tcggggagtt cgcgctcact
    33601 gtcgagtgtg tgaatcgtcc cttgggggcg aaggcagtcg tgtacgacga cctccttgaa
    33661 gacacgctgg agacggcgcc gcgcttccgg ctctaccgtt tcgaggggca gatcggggat
    33721 cgggcgatct tccgggtcaa tcggacgagc ggggaggtaa acccctgcgt ccgggtgtac
    33781 cgacccgacg ggagtctgct ggcggaagcg gggccgctgt ggccgaatta cgagcgggcc
    33841 gagttgttcg atctgaatct ctcccagacc gggactcaca cgctgcttgt ggccgatgag
    33901 gacgggtacc agctgggcca gtaccatgtc acgctgcaat gtgtcaaccg gccggttggc
    33961 gcagtgacgg ccgggtacgg tgaacttgtc agcggtcagg tggccgatgt gccggagatg
    34021 tacctgttcc gttttgacgg cgcggtcggg gaacaggtgt gcatacggtt gaaccggacg
    34081 tccggcgact tgaacccgtg ggtgcggctg tatcggcccg acggcagcct gctggcggaa
    34141 gcaggtccgt tgtggccggg ctatgagcgc accgagctct acgaccagct cctctcgcag
    34201 acgggcacgt acacgctgta tgtgagcgac gaagacgggt accagacggg ttcctacgcg
    34261 atgatgcttg agtgcatcaa cgatccgggc gaagtttacc cggccgccta caatgatctg
    34321 atcgcggata ccgtcgatgc ggtgccgcgg tggcacgtgt accggttcgc cggacagagc
    34381 ggggataggc ttattgtccg cgccaaccgg atctcgggcg aagtgaatcc gtggattcgg
    34441 atctatcgtc cggatggcag tctgctggcc gacgcgggac cgatgtggcc cagctacgac
    34501 cgcgccgagc tgtacgacgt taccctgaat ttctcgggga cttacacgct gtacgtcagc
    34561 gatgaagacg gctaccaaat gggggaatac ggcgtcacgc tccaatgtgt caaccgtccg
    34621 ataggggcgg ggaccatggc gttcggcgac gtggcggcgg aggtcatcga ctcgtttccc
    34681 cgcatccggc tgcaccggtt tttggcgacg gtcggggatc gggtagtaat ccaagcgtcg
    34741 cgcgtgtcgg gggatgttgt gtcgtgggtg cgcctgtacg atccgagcgg caatgttgtc
    34801 actgagggcc aatcggacgg tgcggtcacg atttgcgccg cgctcatccc ggccaccggg
    34861 cagcagacga tctacctgag cgacgacgac gggttcggtt tggggaccga caccctgaga
    34921 cttctcgaat tcaagtgcgg cgatgtcaat acgatgagcg gaccggcggc catcaacgtt
    34981 tccgacatca cgtatttgat tgcctggacg ttccgagggg ggccgccgcc gatgtgcctg
    35041 caggctgccg acgtcaccaa cgacgggggg ttcacggttg ccgatctcac ctacctgatt
    35101 tcgtacctgt ttcggggcgg tccggcgccg gcatgctagg cgacgcggtc gcgggaattg
    35161 cagcgcctgc ggtcggggcg gccggctgca ggcgagttct aaaggggccg cgccaatggg
    35221 caggagggtg aggggacgtg cgggcggggg ctttacaagc gccggggttt gccgtatatt
    35281 cgaagctctt agctcaccgg ccggcgggac cggccggtgc caggaagggt atggccgtgt
    35341 gaagaaaaaa atccgcatcg ggaatgccgg cggctactgg ggggacgatc tcggggcgct
    35401 gcgccgtcag ttgaccggcg gaccgctcga ctatatcacg atggatttcc tggcggagat
    35461 cacgatgtcg attctccagc gccagcgaaa agccaatccc gacct
//
LOCUS       DAOPOE010000020        34599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1644_length_34599_cov_5.843736, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000020 DAOPOE010000000
VERSION     DAOPOE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34599
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_34599_cov_5.843736"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="lptB"
                     /locus_tag="PKM94_04520"
     CDS             <1..668
                     /gene="lptB"
                     /locus_tag="PKM94_04520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOD65936.1"
                     /translation="GVSIEVKQGEVVGLLGPNGAGKTTTFYMIIGFIRPDGGKVVLGG
                     KNIGRLPMYKRARRGIGYLAQEASVFRKLTVEENILAILQFQGLGRKDRRRRLEELLA
                     ELDITHLRKHKAYTLSGGERRRVEITRALVNRPKFMLLDEPFAGIDPIAVEDIQSIIA
                     RLVERGLGVLITDHNVRETLSICDRAYIMCDGTILKAGTADFLANDPEARKIYLGEKF
                     RLN"
     gene            827..2251
                     /gene="rpoN"
                     /locus_tag="PKM94_04525"
     CDS             827..2251
                     /gene="rpoN"
                     /locus_tag="PKM94_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOD65937.1"
                     /translation="MKLNVGLSQRQTLRQSLVMAPQLIQSLKMLQMPALKLEQKIRQE
                     LAINPMLEEVEELEAEQDVEVEQEAEFDVAEPERAEEKAEVDWDQYLFDDEEGYKVRE
                     TRESDEDRFEGTAAASANLYDHLNEQLMLLKLSEEEKLIGEYIIGNVAPDGYLSISVP
                     EMAKELKTEAQKIQAVLEMIQRFDPTGVAARDLRECLLIQLREKALEGTLAWRIVDEH
                     INELDRKSILQVAKMMNVPVERAQAAMETIRSLSPKPAQGRFDPAAMPVVPDLIVERI
                     GDEFVVYHNDSHIPRLQINSGYKSLMRRGRGASPETREYIKQKLEQARWLLNAINQRR
                     STMIRVMEAIVERQREFFERGQAFLRPLIMEDVAQMVSMNVATISRVSNGKYVQTPLG
                     VYEIKYFFNSGIQRENGDEMSKRSVKQRIEEIIAAEPPDKPLSDQEIYRILNTEGIKL
                     ARRTVTKYREELKIQPARLRKRVG"
     gene            2510..2869
                     /gene="raiA"
                     /locus_tag="PKM94_04530"
     CDS             2510..2869
                     /gene="raiA"
                     /locus_tag="PKM94_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOD65938.1"
                     /translation="MLKKVTARHFDLTPEIRAKAEAEMDGLTRFFDNIVSAEMVLDAE
                     RHRRVAELKVNVFNNLIQGRGETDDMYNSIDLAVDKVKGQLKKYKGKLKHRKPEEVAE
                     VKESLTRPTTDVDDVEM"
     gene            complement(3056..4033)
                     /locus_tag="PKM94_04535"
     CDS             complement(3056..4033)
                     /locus_tag="PKM94_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65939.1"
                     /translation="MMRYLDYDAFRAFLSRRCGSEAERRARSVGVDRAHDSGYRDIRR
                     FFGPEAVDPGRVALAYRETPFRLDDPLAASFARRIETQLRAEGRLHRGPMIAAVAEEH
                     FDSPSPRLVLQPCDYGLFAGACFALDLPNSVFSPAGGTLREYHHARGLRLPAGLGVCG
                     LLVTEEPDRTRRVLVVRRAAHLASLTGSVGPSAAGSVDYGVKWKTLEEMARAAMAAEI
                     AEELGLTPAEYAITLLAYAGELFRGGKPQLFCRIDTSLSAATVADRLDAVVARLARDG
                     RLAEHTDFEFLPLDGAARLSDTDVARLNHEAAMNWRLAEESAASTSPGR"
     gene            complement(4044..4973)
                     /locus_tag="PKM94_04540"
     CDS             complement(4044..4973)
                     /locus_tag="PKM94_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD65940.1"
                     /translation="MPYVGEIAGLATSALWAITSILFAEGGKLIGSFKVNNIRLVFAA
                     VIYASLLTILTGLPFPQDINSRQLFWLGLSGLVGLVFGDSCGFKALVMIGPRLTTLMY
                     ATTPIWTTVVAWIFLDERLNWLDILGIVVTIAGIVWVISERRFKKIVPNDIGESHPDA
                     GSLFTGLLLGVGAALGQAVGLVMAKQGMVHAGGAVEPIEASAVRMGVSLVAIWLISGL
                     RGQIPATVKAARHLRAMKFTLGGAVFGPFLGVWMSLLAVRYIEAGVASTLNSMSPVMI
                     LPLLIIIYRERPSLRAWLGAIVAVIGVAILFLN"
     gene            5180..7471
                     /locus_tag="PKM94_04545"
     CDS             5180..7471
                     /locus_tag="PKM94_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65941.1"
                     /translation="MSALWKIAIAALLLGAALPARGAQWSLAGEVVDLDGAPVIGANV
                     AVWIGSTPVAAGAADEHGRFALRLKDSTAAPLRLVVSSVGYLRHESTVAGGGLPGRLR
                     IELRPAPVAVEGVTVQPPEAAGAPCRALTRAEVAAAAQTSFVPGNPIDAVKEPRVLRE
                     GSSHSARLRVSGSSPVYTINGIAIGRNPTHYGMFAVVPAAVVERMTVRTLGTGAAHDA
                     PAAIDLALPRPYGRHRDASLSLSAIDATAAWSLGGPHWFTLGAARKSVLDEIVNRLDT
                     DAGRRTVPPTNFQDLVASSGLRLGSAASLAIDQYHVHDALEYRTGGTARNTRGAQTAL
                     ATAEHYIGARLEMLRSGTLLRLQAAVQSTEESYAAASVPDGDEAEPPILDLHASGVTT
                     TIAGEVVLSLGACELTIGDRVRHVMKQRIDLTQQNWNFLPPDAASDLPNPYQPELNRL
                     YGSYHGTRRRTDHAAYASLRWRAGPVHMESGARIGHFGGLARKEWAVFRQAATLDLDD
                     KGALTFFAGTFAEDPAQRVLEAYQALVHTDYNGLQPVRTALVSLQYTCGPASAALYRK
                     QIAGLPVLTPDFRRVAEDGAAGEGFLRTPSIGEADFAGGDITLAFDSLAGGRLALTAS
                     YGYTAGTKTTASVTAPSEFNAPHRWYAMARYRVSRVVAVGAEAAVRSGYAYTLPPKSP
                     AAPDGDRLTVDYYRAALARENGARFPTHATLNLNAVFDFGAAEAYCAIANITNRANPI
                     IRSADGFIYDAGILPSAGVTVRF"
     gene            complement(7501..7776)
                     /locus_tag="PKM94_04550"
     CDS             complement(7501..7776)
                     /locus_tag="PKM94_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65942.1"
                     /translation="MSALTYVLTLGALIGVVWHVIATMAIYNYLRRRGDKVSFVWLRL
                     MSPIYASRYRAATRQETGRVGHLFYHWVIPINLAFLLVVLAVASRYV"
     gene            complement(7922..8524)
                     /locus_tag="PKM94_04555"
     CDS             complement(7922..8524)
                     /locus_tag="PKM94_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65943.1"
                     /translation="MEENRYKLAGWLAIVQAVLFPVAIVIGLIEAGVAAEFFNIRRPF
                     VGPSDFLMIPFTAIAVYTLLKFRSLLHERYAYRDLDLLIIVSVWWTIAFQVCGLAFGI
                     LTSAFWPVDRVVLLVGFVAFFTGAMVSIGVVDILIAVKLLGAKERFGEYIRGFAYVTM
                     AAGICEVTVLMSVLALLLVPVSAIVLALIFFRDQQEVEFV"
     gene            complement(8569..8781)
                     /locus_tag="PKM94_04560"
     CDS             complement(8569..8781)
                     /locus_tag="PKM94_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD65944.1"
                     /translation="MAIQIKLDLILVKKKMSLTELAARIGISVTNLSLLKTGKVKGIR
                     FSTLEAICRELECQPGDILEYEPDFV"
     gene            9055..10044
                     /locus_tag="PKM94_04565"
     CDS             9055..10044
                     /locus_tag="PKM94_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOD65945.1"
                     /translation="METFLQNHYLLYGAFTAFVLGMLALDLGVFHRRDHVVSMRESFA
                     WTAVWIVLALAFMAFVYYRYETVSPGRGTPAALEFLTGYLIEKSLSIDNVFVFLLIFS
                     YFNVAPIYQHRVLFWGILGALVFRAFFIALGALLIAKFHAIIYLFGAFLVFTGIKMAW
                     AKDKQFHPERNPVLRLFRRFVGVTKDYRGKRFFVRAAGRRLATPLFVVLLLVESSDII
                     FAVDSIPAIFAVTKDPFLVFTANVFAILGLRSLYFALAGVMRLFHHLHYGLSVILVFV
                     GIKMILSDIYKVPIGISLGAVGLIIAVSIAASLVWPKRAVAPGDPPALPEIPA"
     gene            complement(10073..10297)
                     /locus_tag="PKM94_04570"
     CDS             complement(10073..10297)
                     /locus_tag="PKM94_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOD65946.1"
                     /translation="MRRLVVAVIGFTVLALGVALLVLPGPAFLVIPAGLGILSLEFVW
                     ARNLLRRLRRKLTGQPPADPAPPRSTGLGG"
     gene            complement(10392..12818)
                     /gene="gyrA"
                     /locus_tag="PKM94_04575"
     CDS             complement(10392..12818)
                     /gene="gyrA"
                     /locus_tag="PKM94_04575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD65947.1"
                     /translation="MERQKIETIFLEEEMKHSYLDYSMSVITNRALPDIRDGLKPSNR
                     RILVAMNDLNLAPGRPHRKCAKICGDTSGNYHPHGEQVVYPTLVRMAQDFNMRYPLVD
                     GQGNFGSIDGDGAAAMRYTEARLTPMAMEMLDDLKKETVDFMPNYDSTREEPVVLPAK
                     FPNLLCNGTSGIAVGMATNIPPHNLNEIAEAIIRVIDDPECTNDDLMEIVPGPDFPTG
                     GIINGRDGIREAYRTGKGHVYVRARAAIERMKNGKDAIIVSEIPFQVNKTNLLEKIAE
                     LVRDKKIDGIADLRDESDRDGMRIFIELKRDIPPDVVLNQLFTHTTMQTTFSVNMLGL
                     DHGVPKQMSLKQLIRAFVDHRHEVVVRRTQYDLRKAEERAHILEGYRIALDHIDEVIA
                     LIRKSKDTPTAHAGLMKKFGLSDVQATAILEMRLQRLTGLERQKIEDEYRELIQKISE
                     YRAILESQALRMQIVKDETRGLAEKYGDARRTEIQDAAEELSVEDLIAEEEMVITISH
                     QGYVKRLSVSGYRRQQRGGMGVRGIETKEDDWAEHLFVASTHDYVLFFSNKGRCYWVK
                     VHEIPVGGRLAKGKPIVNLVQMEKDENITAFTRVRDFSPDRFVVFATRQGVVKKVALD
                     SFSHPRVTGVNAITLPVEDELIEACLSDGTYDIILATRKGMAIRFKEEKVRAMGRTAY
                     GVKGITLGKKDYTIGMVVVKRDSTILTVCENGYGKRTSVDEYRITNRGGKGIINIKSS
                     ERNGEVVAIKEVLDQDELILITRKGVTCRTAVKGISVIGRNTQGVRLMQLRDDDTVID
                     VAHVVTDESQ"
     gene            13176..14525
                     /locus_tag="PKM94_04580"
     CDS             13176..14525
                     /locus_tag="PKM94_04580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD65948.1"
                     /translation="MLVYAVVTAYRTWLVALLLAAVIVAAVKPAVEVGVRQVLPPVRQ
                     SKMLGLVKREKPRPAALAVTRLLMLAAWAGVGGVVFLRWRRELPDRLAAAEACARRWE
                     HEAERLAETAPDRSRRLYRAALVLTLDREARRRVQARLCAPGGAADAAATCAATPADT
                     LAPPTRAGAEGPAPPGLDRRYRLEEKLGQGAMGIVYRGHDQVLDRPVALKRLAARLVG
                     ESVYSERFRQEARALARLSHPHIVQVFDLIAVGEEIWMVLEYLDGGSLADYLKTHGRL
                     EPAEAARMICGIADGLAVAHREGIIHRDIKPANILLTADRRAKLSDFGIAKLAREATL
                     TQAGSTLGSPAYMSPEQCSGEGLDERSDIYSLGITLYELLAGRPPFAGETSGILAQHL
                     VGDPAPLAQWAPGVPEPLDRLVRLMLAKRREDRPQMMAEVAAALDGWTERTAPVPGA"
     gene            14692..16329
                     /locus_tag="PKM94_04585"
     CDS             14692..16329
                     /locus_tag="PKM94_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD65949.1"
                     /translation="MKACVRAALLAGVAALASTAGASRLTDLTGWWRVIEDNGHFIRL
                     VQQGVDVTILDGDGTLIAPGVFANDTLWAYPTPADTVILVYHGDTLSGLNEEGNPMTI
                     VRYPVDLNGWWANVDGGREIPRFVHEGSAVQLWMDTDTSTWFVADGTFANDTVLLDLP
                     PELGGALTLIYRADTLGGMGGPDADSIILARSPYGKWMPIRCGTVAIGGDFEDWPYMY
                     LMAQDRRDDGTSTPAAELDRVYLCHDSDYYYFRIECVGDAAFPHSGTLWDRYSIVWYI
                     SGTDSTCSIRIESTWEMYFWNNMTQQETMLEPLAVSGRVIEGRIPLELLGRLAEEAEV
                     SVRSAYYDWDFGWLSYDEIQFNAARQACGADPARLTPQPQYAFLSNAVTPVMDTLVIG
                     NLPDRAAGDIRLSSLRVNGAVQPTVCAGHLKCPGFAGSVVTALFPARTFLQGYGLVWD
                     TARAPYTVTGEFRDGAPVNIEGEVTIIGHRSGDVNGDGTASVADVTFLVGYVFRGGRA
                     PQPVELGDVDCDGGITVTDLSSLVAYLLRGGPPPCAR"
     gene            16495..17004
                     /locus_tag="PKM94_04590"
     CDS             16495..17004
                     /locus_tag="PKM94_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65950.1"
                     /translation="MGTMVRRILLSALLGAVLAAAARPAAKFDLKGLAGSWEGEGMFL
                     MPVTDLEVEVEGEGEFVYDAKRDLVKTTMSGSKFLVPYSDSGLLRYDLATDSVSWEVW
                     DNWGQHSMYYGQWRDGVIAADRLWKSRNYQVTVTFPHRDTLNFHLTATDPQTQLKTTR
                     ARFSLWRVK"
     gene            17008..17523
                     /locus_tag="PKM94_04595"
     CDS             17008..17523
                     /locus_tag="PKM94_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65951.1"
                     /translation="MAAWATTVDALVRNFLSALNASVPSMRAARILDDDLTGFDDWER
                     LCDTLYRLLVIEPIRASLPKPAQAVFDLPSYETQYESLEDFSLIQVTPPGKAAPPRQI
                     AGTAVFFRFLPGNEQTGRFDLVETVRVNEGLTIEETSWETARAEEVYFTCLVPERQGW
                     RLLDHVQVEME"
     gene            complement(17645..18265)
                     /locus_tag="PKM94_04600"
     CDS             complement(17645..18265)
                     /locus_tag="PKM94_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOD65952.1"
                     /translation="MRNVRVRRGVLTAAAALCFASGAPQADRSAKAERPCNHSVTIDA
                     VTYDVPERWCERYVPPHERAAADTLVALPKDLCDNGSRIYVTRATREAFVRMAEAARK
                     AGIRFRVDSGYRSPAYQKRVIRRRMAEGQTFHQVVRFVAPPGFSQHHTARALDLVPSE
                     ARFVHTDAYKWLTRHAADFGFVESYPKPVEKSDYDYWESWHWYYRE"
     gene            complement(18267..18791)
                     /locus_tag="PKM94_04605"
     CDS             complement(18267..18791)
                     /locus_tag="PKM94_04605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOD65953.1"
                     /translation="MLADRKQHATCERVIAMADDIKKVLRKFEYGVYVVTMGKGENGN
                     AFTASWVTQVSSDPPLVAIAVHKKRQSIPLLREHQAFVVNLIGEGQEAVAKSFYGPAE
                     AGYDKLKARTLGNSPATGTPVLHGILGYVDCAIVNSVETGNHILFIGEVKAAGLESDR
                     QILTGTNSGLRYVG"
     gene            complement(18845..19786)
                     /locus_tag="PKM94_04610"
     CDS             complement(18845..19786)
                     /locus_tag="PKM94_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD65954.1"
                     /translation="MSSSDRRPFEPNLERCACCPRGCGADRTASPLGWCRSGADLSIG
                     SICAHRGEEPVISGPHGICNIFFTRCNLQCVYCQNYDISRTHEPIIETAMSYDRLADR
                     VEEHLDRGCPSVGFVSPSHYYPQMLRIIDVLAARGRRPVYVCNTNGYDRVEAIRRLEG
                     IIDVYLPDFKYMDERLAREFSQAPRYPEIAAAAIGEMFRQMGAEIELGADGLIRRGLV
                     IRHLVLPGQVQNSIEVLRCIAECFSPDCHLSLMSQYWPTPAVARHPTLGRRVLRDEYD
                     EVLEAMERLGFTRGWVQELGSSEHYRPDFMRDHPFEP"
     gene            complement(19889..22837)
                     /locus_tag="PKM94_04615"
     CDS             complement(19889..22837)
                     /locus_tag="PKM94_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOD65955.1"
                     /translation="MMRSLRCATVLLLLVLCASTVVAVNEHYLRLKADSPKALAELSE
                     VVSIDNVQDGVVLAYATDEQLAALERLGYAFEELPRPSTLIVPEMTSDLRDARAWDVY
                     PTYDAYVAMMNQFAATYPGLCQIVTIGTTTEGRALLAAKISDNVAVDEDEPEVLYLSS
                     MHGDETTGYVLMLRMIDSLLSAYGVNPQITSMVDNLEIWINPLHNPDGTYAGGNSTVY
                     GATRYNANGVDLNRNFPDADDGPHPDGNAYQPETNAMIAFAQARHFIISANFHGGAEV
                     VNYPWDTWSRRHPDDAWWQTVSHVFADSAQYYSSGGYMDGFNDGITNGYDWYTITGGR
                     QDFMNYWHGCREVTIEISETKLLPAGQLPTWWGYLRVSLFNYLRNALRGVRGIVTDAN
                     TGLPLAATIFVLSHDTQADSSQVYTDPVLGNYHRMIAPGTWNFVYSSPGYYPDTVFGI
                     TVASYAGTVTQNVQLQPLPNAPVLTFVSQTAGALDPGESAAMSITLKNEGAGNAVNLA
                     GTLATADSYITITQNSAMFPTITALGGTGTSLTAYQFSVSPSCPLEHPVDFVVHLTAD
                     GGYSDSVLFSLTVGQTIEDFETGNFAAFPWTQSGNAAWVTTTSAPYEGTYSAKSGTIS
                     HSQTSTMQVTLSNLEAGTVTFRYKVSSESGWDYLEFWLDGVRKDRWSGTVAWTEASYA
                     TTAGSHTFRWTYTKDGSQSVGSDCGWVDYIVFPTADPDPDNDGIPTGSDNCPTVANPG
                     QTDADGDGKGDACDNCPSVQNPTQANSDSDTFGDACDNCPTVANQIQTDADSDGKGDA
                     CDNCVTTPNPDQANSDTDAFGNACDNCPAIANATQNDADSDGVGDACDNCAAVPNTSQ
                     TNSDGDVRGDACDNCPTVTNPTQVDGDQDAVGDACDNCPEIANPDQADGDLDGVGDAC
                     DYLCGDVTGDGSFTVGDLTFFIAFMFRGGPSPANMAAADVDGQVGITVGDVTYMIAFL
                     FRGGPALVCQ"
     gene            complement(22960..27507)
                     /locus_tag="PKM94_04620"
     CDS             complement(22960..27507)
                     /locus_tag="PKM94_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOD65956.1"
                     /translation="MRFLRFMLVVTATALSGAVTVAAQVSEGGTPPSFGKAVGAAIDR
                     YVTAPIDVAALLEEDARTPKDVPFRFGYPFDVRLGLDNAGTWEVLADGSRLWRLQIEC
                     PGAASINLIFDRFWLPDGARLFIYNADRSHVIGAFTSRNNKDYGSFATQPVRGDVSVL
                     EYWEPAGLNAQPELRVSRIVHAYRNLFARDFLKDFGESGACNNNVRCPEWAAFDPLIR
                     SVALITTGGGFRLCSGAMINNVRQDLTPYFLTANHCLGGSDTWVFIFNYESPACSNVD
                     GPTYMTVSGSTLRANLAYSDFALLELSEQPPDSYNVYYAGWNAVNAAADSVIAIHHPS
                     GDIKKITFDYNAVTSTSYLGTAVPGDDSHWRIGQWEDGTTEPGSSGSPIFNRQGQIIG
                     QLHGGYASCASITSDWYGKFAKSWSYGSTAATRLRDWLDPDNTGALSLGGRESAGISI
                     THDPLPDTRDTVNDYAVLAAITSDVPLDPDSLLLYYEIGAANYEDTLTPTGNPDEFVG
                     YIPAQAPGTVVSYRLLAVDSDGKADTTDSFSFRVIDYGLTLAPAADTLSGPVADSVWY
                     ALQIVNTGVYTDSYALAVSGNAWTTEIFDAAGTTPLLSTGPLAADSVFAFTVRVIVPP
                     SVYGAADTALVTATSVGDPVYTAASRLRTVSDGEPLAIPFEDEFPAAALSAAHWVQTT
                     GAEANAVALAEPSEPYSLNLDGAPAGADTVMSQVINLAGQGNLIVRYFYERTGGGDSP
                     EANDDLFVEYRDSAGVWQLLQQHLGSGPDMTTFSEVELPLPPAAYHSAFRLRLRSIGT
                     AGAYDDWFVDDLYVGPPQPFKLSLDPAFASQYGPAGDTMQYTLQIANRGSQADQYSFA
                     DSGGAWPVLFWDDAGVAPVGGSGMIAAGDSARFVVKVEVPADAPMNAVDSCRVRAVSA
                     GDPLMFDDSRLVSFSAGPPGGFPWYEPFPVDTVIVSRWTSIVGAAVSAAALFPPSAPY
                     AAALDGGVDTLTSQPIDLSGRSDVVLSYYYERGGSSEPPDAGDDLWVEYKNNSGSWVL
                     VARHLGSGAPMSSFERVTFGLPADALHGGFQVRFRSFGSGADSDFWFVDNISVDYAPA
                     LAVAPSGFSAGLVQGDSAADRLLIANTGLGVLTYSLSVVPVAAKGNTRLGELLASGEL
                     APAHRRLAEGFEDYADHKGADDPRSGPAVEKSAGGPDGHGYFWIDSDESGGPAFEWID
                     VSAAGTDVISALNDDNFAGPYAIGFPFPFYDSAYTEIYIGSNGIIGFAPGAMNSRFKK
                     HIPNDTLPNALLAWLWDDLNPDDVNNAAAAHVYLDTSGGRCVIQFTAYPEYLANPGDV
                     ITAEVILEPDGSIRFQYLSVAAGFDAAGGTIGIESPDGTDGLEVAYLTPYLKDSLCIR
                     FVQPYQWLGLSRFSGTVAAGGTDTVGLMFRSAGLDSGLYQAALVVSCNDPNHPAETVP
                     AELTVTLQPAWICGDINGDGAGPSVADLTFFINFLFRGGPAPPDPAACDFDGSGDIAL
                     PDLTQLINFLFRGGSPPTCGN"
     gene            complement(27924..28424)
                     /locus_tag="PKM94_04625"
     CDS             complement(27924..28424)
                     /locus_tag="PKM94_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOD65957.1"
                     /translation="MMITKKMAKALNEQIGAELDSEYIYLAMAYKFEEMNLTVFAKWF
                     YKQAEEEHKHALKFAKYLVEQGAPVTVPGIEAPKAAWKSAQEICDAAVEHEKYITKRI
                     HDLVKLARAEGDIATEQFLQWYVAEQVEEVASTSELAGMVKLAGTAGQLLQLESRIYR
                     MVEARS"
     gene            complement(28625..29986)
                     /gene="rsmB"
                     /locus_tag="PKM94_04630"
     CDS             complement(28625..29986)
                     /gene="rsmB"
                     /locus_tag="PKM94_04630"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOD65958.1"
                     /translation="MAEQIERKSPQRFDAVRAAAIEALVLIEQGEQTDIAVQQVTSGR
                     ALRPIDKRFLLQLVNGTTKMRRRLDHEIKFYLARPSRELPLKLANILRLGFYQLHFTD
                     RIPAAAAVSESVNLAHYMTDRSRANLVNAVMRSAIREPQKVRFVSKDEDPAKYLGDFY
                     SYPEHFVRYCLTEFGFDKTEQLLRVFNAPPHVTYRVNFLKAKPDEVANILQSNNIEFS
                     YGKYLPEFIHIEESGLPLQGELIDTGKVFVQDESAGLCARLLNPQPRTNVIDLTAAPG
                     GKATYLAIRMRNKGRVTAVDKSHARLQVLVDNAQRLGIKIIAPVVSDMLDFHGGPFDR
                     VLLDPPCSGWGTAGKNSDLRWQKSAEDIANLAKIQAKMIDHAATLVKPGGVMVYSTCT
                     IIRAENDQVVEEFLVRNRQFEIDSAARFFPAELVTERGFVKTYPEVGRLDGAFCTRLR
                     RMT"
     gene            complement(29993..32494)
                     /locus_tag="PKM94_04635"
     CDS             complement(29993..32494)
                     /locus_tag="PKM94_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOD65959.1"
                     /translation="MKRLLEERFFPYVVKPGRYAGGEPGSLVKAHEGRLRYLLAFPDK
                     YEIGHSYLGLQTLYHVVNQDDRFVGERVFAPDRDAEEIMRKHGIPLFSLESRTPAAAF
                     DAIGFTLSYELVYTTVLQMIDLAGLPLRAAERTDEQPLILAGGPAAYNPEPMAPFVDL
                     FFIGDGEEGLPQMLAILHQMRGATRAEKLARLVREVESVYVPRFYDDRRRPLVDFAPA
                     EIAARVVPALKPSYYPPQPVVPLIETVHQHLSVEIMRGCPRGCRFCEAGTIYKPVRLR
                     PPHEILAQVETQERHVGADSVSLLSLSTSDYPDIDDLARKVARLLEPMRAAVSVPSLR
                     PGSITPELLDTLKLVRKAGLTIAPEAGTERLRAFIRKDIPDAAVYDSAEIAFSKGVTT
                     LKLYFMIGLPTETEEDLLAIARMIERVFDLSRNYPGKRTVNVTLSPFVAEPHTAFQWD
                     ATATPAEIDEKLKFIKRHVRSRHVNFKHGSAEEAVLKALLSRGGREMADVIEAVYRAG
                     GRFDGWAEDFNPQRWFDLCRSRGIEIEERLRSVPFSADLPWSHIRKGPSLAHLQEERH
                     RTSTQLREYVPRFRGETAAPLPEPELQFGRGKKKVATRATAAPTRNRLRIRWGKTERL
                     RFMSHLDNLRLIERTIRRSRIPIAYSQGFHPTMKLSFGPPLPLGFTSEAEYFDLTLET
                     NFMPYLLDKVRAAMVEGMAMYEAGVVLGKAPSLSSQLNRVEYTLVLDDDADLAAVRPR
                     AEELLGKARVEIERTGKNGPTAVDIRPAVYAITVADRTVEMVLGVGEGGYVRPDEVLG
                     ELFTAERAKALAHRLHRRAAYRIDEAGQRIDPMQL"
     gene            complement(32658..33182)
                     /locus_tag="PKM94_04640"
     CDS             complement(32658..33182)
                     /locus_tag="PKM94_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD65960.1"
                     /translation="MTCRECRDLLPALVLGEVSESDRPAVMNHLAACAACRSEFECLQ
                     SICSALTSDGEGGLSELERLRLENTVWRRLATPESAASGGGRFARYALRVAAALVLFG
                     LGYFVRPHLGGAPAPAQPVASDRLEATLAALDRRELTRDMRFSATGFKVIAHGRKAVI
                     EKLESASLDRPRLP"
     gene            complement(33179..33685)
                     /locus_tag="PKM94_04645"
     CDS             complement(33179..33685)
                     /locus_tag="PKM94_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD65961.1"
                     /translation="MSEDVAAAMRRLSDGAFGLIRRMVRDREEALDLTQDVLVRVLSR
                     RRPDSPADLKAYAMRAAYRAALNALRDRKRHARIHDRLAAEQRSRPDNPDAIETAERK
                     RMIAEALGELPPKQREAISYRFFGELAVAEIAAAMGISEGAVKVHLFRGLANLNAQLA
                     DKMKEMSI"
     gene            complement(33707..>34599)
                     /locus_tag="PKM94_04650"
     CDS             complement(33707..>34599)
                     /locus_tag="PKM94_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65962.1"
                     /translation="AQVLQSPLALPSAPAWTIIEQRVDSTQAQPELHLKASRTVPYGA
                     PLPESYVTAETPSPELDLHFPTAVTVRTEGNRTYYEFRRTYQARRFGEYDITESPWLW
                     DADLETKAIDSGLVNLSPDERRKYLEQLAAAFSLKHWRMNRNALAELVRSGSLEAATM
                     DLIAARAAEHFERTLTTEFLFRTMQQSDETVTLTLDSLEAAFAETFRARLAEAVGTPA
                     AAVLEQFEAALERVHRDQRITEVLSSDEFSIRLNLPGTIIESNGLLSPEEPGTVRWSF
                     EGKKLHDADLPLYALSVVAR"
BASE COUNT         5907 a        10685 c        12049 g         5958 t
ORIGIN      
        1 ggggagtctc aatcgaagtc aagcaggggg aggtggttgg gcttctggga ccgaacgggg
       61 cgggcaaaac cacgactttc tacatgatta tcgggtttat ccgtccggac ggaggcaaag
      121 tcgtattggg cggcaagaat atcgggcggc tgccgatgta caagcgggcg cggcggggga
      181 tcgggtatct tgcgcaggaa gcgtccgttt tccgcaagct cacggtcgag gagaatatcc
      241 tggcgattct ccagttccag gggctggggc gcaaagaccg gcggcggcgg ctggaagagc
      301 tgctcgccga gctggatatc acgcacctgc gcaagcacaa ggcgtacacg ctgtcgggcg
      361 gggagcggcg gcgggtggag atcacgcggg cgctcgtgaa ccggccgaaa ttcatgctcc
      421 tggacgaacc gttcgccggc atcgacccga ttgccgtgga ggacattcag agtattatcg
      481 cccggctggt cgaacgcggg ctcggagtgc tcatcacgga ccacaacgtc cgggagacgc
      541 tctcgatctg cgaccgggcc tacataatgt gcgacggaac gatcctgaaa gcggggacag
      601 ctgactttct ggcgaatgat ccggaagcgc ggaagatcta cctgggagag aagttccgat
      661 tgaactgaga gcatcctgcc gggtcggacc gccaaccgac cgtctgtcta ataaatgaac
      721 gaacggccgc ccggtcgtaa acacccgccc gatcgattcc gatacaccta gcagagaggc
      781 acagaccgca ccgtcggatg gtttgcatga taacgggtta ctggcaatga agctcaatgt
      841 cggattgtcc cagaggcaga cgctcaggca gtcgctggtg atggcgccgc agctgatcca
      901 gtcgctgaaa atgctgcaga tgccggcgct caaactcgag cagaagatcc gccaggaact
      961 ggcgatcaac ccgatgcttg aggaggtcga ggagctcgag gccgaacagg acgtcgaggt
     1021 cgaacaggaa gccgagttcg acgtcgccga gccggagcgc gccgaggaga aggccgaagt
     1081 ggactgggac cagtacctct ttgacgacga ggaggggtac aaggtccggg aaacccggga
     1141 gtcggatgag gaccgcttcg agggaacggc cgcggcttcg gccaacctct acgaccacct
     1201 caacgaacag ctcatgctcc tgaaactctc cgaggaagag aagttgatcg gggagtacat
     1261 tatcgggaac gtcgcgcccg acggttacct gagcatcagc gtcccggaga tggccaaaga
     1321 gctgaagacg gaggcgcaga agatccaggc ggtgctggag atgatccagc gctttgaccc
     1381 gaccggggtg gccgcccggg atcttcggga gtgcctgctc atccagcttc gggagaaggc
     1441 gctggagggg acgctcgcgt ggcggattgt cgacgagcac atcaatgagc tggaccggaa
     1501 gtcgatcctc caggtggcca agatgatgaa cgtgccggtg gagcgggcgc aggcggccat
     1561 ggagacgatc cggtcgctct cgccgaaacc ggcccaggga cggttcgatc cggcggccat
     1621 gccggtggtg ccggacctca ttgtcgagcg gatcggcgac gagttcgtgg tctaccacaa
     1681 cgacagccac atcccccggc tgcagatcaa ctccggctac aagagcctca tgcggcgggg
     1741 gcggggcgcc tccccggaaa cacgcgagta catcaagcag aaactcgagc aggcgcgctg
     1801 gctgctcaat gcgatcaacc agcggcggtc caccatgatc cgggtgatgg aggcgatcgt
     1861 ggagcgccag cgggagtttt tcgagcgcgg ccaggctttc ctccggcccc tcatcatgga
     1921 agacgtcgcc cagatggtga gcatgaatgt cgccaccatc agccgggtat ccaacggcaa
     1981 gtacgtgcag acgcccctcg gcgtgtacga gatcaaatac ttcttcaact cgggcatcca
     2041 gcgcgagaac ggcgatgaga tgtcgaaacg ctcggtcaag cagcggatcg aggagatcat
     2101 cgccgccgag ccgcccgaca agcccctgtc ggaccaggaa atctaccgca ttctgaacac
     2161 cgaggggata aagcttgccc ggcgtacggt gaccaagtat cgtgaagagt tgaagatcca
     2221 gccggcccgc ctgcgcaaac gggtgggctg agcgaacgac ccggcctgtt tccgcgggcg
     2281 cccgcagcga cgcccgccga gagagagtcc atcggatgga tcagtcaagt gcagtcgcca
     2341 ccgtcggcag cggccgctgc cgaacctctc tgtcgccaca accgtatcaa ctagtgagga
     2401 gtgcgtgtcc ggaccccggt ccggctggct gcgcctgccc cgggcaggcc gtcatacatg
     2461 gagccgcgag aacatttcct gtcgcgtctg ttgggaaagg agcgagcgta tgttgaaaaa
     2521 ggtaaccgcc cgtcactttg acctcacccc ggaaatcaga gcgaaggccg aggccgaaat
     2581 ggacggcctc acccgctttt ttgacaacat cgtctcggcc gagatggtgc tcgacgccga
     2641 gcgccaccgg cgcgtcgccg aactcaaagt caacgtcttc aacaacctca ttcaggggcg
     2701 gggggagacc gacgacatgt acaactccat cgacctggcc gtcgacaagg tcaaggggca
     2761 gctgaagaag tacaagggga aactcaaaca ccgcaaaccg gaggaagtcg ccgaggtgaa
     2821 agagtcgctc acgcggccga ccaccgatgt cgacgacgtg gaaatgtagg ctgcgatcac
     2881 agccggaaga ttctgaagac ccgccccgcc aggggcgggt cttttttgcc ggggctgccc
     2941 ggtgacggcg ggccggtcga gccgaaacgg ggcggccaag gccgagtctc ccgttcgcac
     3001 aggcgcgatt tctccggatc aacagcaggc cgcgtgacgg acccaggtcc gaaggctagc
     3061 gccccggcga tgtgcttgcg gcgctctctt ccgccaggcg ccagttcatc gccgcttcgt
     3121 gattgagtcg ggcgacgtcg gtgtccgaga ggcgggcggc gccgtcgagc gggagaaatt
     3181 caaaatcggt gtgctcggcc aggcggccgt cgcgcgcgag gcgggcgacg acagcgtcga
     3241 ggcggtcggc gacagtcgcc gcagacaggg aggtgtcgat gcggcagaag agctgcggtt
     3301 tcccgccgcg gaacaactca ccggcgtagg ccagcagcgt gatcgcgtac tcggccggcg
     3361 tcaatccgag ttcttcggcg atctctgcgg ccatggcagc gcgagccatc tcctccagcg
     3421 ttttccattt caccccgtag tcgaccgaac ccgcggccga cgggcctacc gacccggtca
     3481 acgaggcgag gtgggcggcc cggcgcacca ccaggacgcg gcgggtgcgg tccggctctt
     3541 cggtgacgag cagcccgcag acgcccagcc cggcggggag gcggaggccg cgggcgtgat
     3601 ggtactcgcg cagcgtgccg ccggctgggg agaaaacgct gttgggcaag tcgagcgcga
     3661 agcaggcgcc ggcgaagagg ccgtagtcgc agggctgaag cacaagacgg ggggaggggg
     3721 agtcgaaatg ctcctcggcg accgcggcaa tcatcgggcc ccggtgcaga cgcccctcgg
     3781 cgcgcaactg ggtctcgatg cggcgggcga acgaggccgc gagcggatca tccagccgga
     3841 acggtgtttc ccggtacgcg agggcgaccc ggccgggatc gacggcttcg gggccgaaga
     3901 agcgccggat atcgcggtaa ccggagtcgt gcgcgcggtc gacccccacc gagcgggcgc
     3961 gtcgttcggc ctcggaaccg cagcggcggc tgagaaaagc gcggaacgca tcgtagtcca
     4021 gataccgcat catccggttc ctctcagttc aggaagagaa tggcgacgcc gatcacggcg
     4081 acgatggcgc cgagccaggc gcgcagcgag ggccgctccc ggtagatgat gatcaggagc
     4141 ggcaggatca tgaccggcga catggaattg agcgtcgaag cgacgcccgc ctcgatgtag
     4201 cgcaccgcca gcagcgacat ccacacgccg aggaagggtc cgaagaccgc gccgccgagg
     4261 gtgaatttca tggcgcgcag gtggcgcgcc gccttcacgg tggcggggat ctgcccgcgc
     4321 aggccggaga tgagccagat ggcgacgagc gagacgccca tgcggacggc cgaggcctcg
     4381 atcggctcga ccgcgccgcc ggcgtgcacc atcccctgct tggccatcac tagcccgacc
     4441 gcctgcccca gagcggcgcc gacgcccagc aacagaccag tgaagagcga accggcgtcg
     4501 gggtggctct cgccgatgtc gttcgggaca atcttcttga accggcgttc cgagatcacc
     4561 cagacaatcc ccgcgatcgt gaccacgatg ccgaggatat cgagccagtt gagccgctcg
     4621 tcgaggaaga tccacgcgac gacggtggtc catatcggag tggtggcgta catgagcgtg
     4681 gtcagacgcg gcccgatcat cacgagcgcc ttgaagccgc agctgtcgcc gaacaccagg
     4741 ccgaccaatc ccgaaagacc gagccagaac aactgccgtg agttgatatc ctggggaaag
     4801 ggcaagccgg tgagaatggt cagcagcgat gcgtagatga cggcggcgaa aacgagccgg
     4861 atgttgttga ccttgaacga gccgatgagc ttgcccccct cggcgaacaa gatcgaggtg
     4921 atcgcccaga gggcggaggt ggccagaccg gcaatttcac ccacgtacgg catcgggcga
     4981 atatagggga gggcggtttc tccggcaaac actgttgtcg gcggcagtgt gtccgccggt
     5041 gcataacaag cacctgggtt cataccgagg gcggcccgcg cacttcgtca agtatctgca
     5101 agataacggg atacaaccgg caagcatttt ccggatccgg gcacggcgtt tgtcctgagt
     5161 cccccggaga gaacccccta tgtcagccct gtggaaaatc gccatcgccg cgcttttgct
     5221 gggggccgct ttgccggccc gcggcgcgca gtggtcgctg gccggagagg tggtcgatct
     5281 cgacggcgcg ccggtgatcg gggcaaacgt cgcggtctgg atcggctcca cgccggtggc
     5341 ggccggagcg gccgatgagc acgggcggtt tgctctgcgg ctgaaggatt cgaccgcggc
     5401 cccgctgcgc ctggtcgtct catcggtcgg atacctccgg cacgagagca cggtcgcagg
     5461 cggcggactg ccgggccggc tccgcattga actgcggccg gcgccggtgg cggtggaggg
     5521 ggtgacggtg cagccgccgg aggcagccgg agcgccgtgc cgggcgctga cgcgggccga
     5581 ggtcgcggcg gcggcccaaa catcgttcgt gccgggcaac ccgatcgacg ccgtgaagga
     5641 gccgcgggtg ttgcgggaag gttcgtcgca ctcggcgcgg ctgcgggtca gcggctcctc
     5701 gccggtctac acgatcaacg gcatcgccat cggccgaaac ccgacccact acggcatgtt
     5761 cgcggtcgtt ccggccgccg tggtggagcg gatgacggtg cgcacgctgg ggacaggcgc
     5821 ggcgcacgat gcgccggcgg cgatcgacct ggccctcccc cggccgtacg ggcgccaccg
     5881 cgacgcgtcg ctgagcctgt cggcgatcga cgccaccgcg gcctggtcgc tcggcggacc
     5941 gcactggttt accctcgggg cggcgcgcaa gtcggtgctc gacgaaatcg tcaaccggct
     6001 cgacaccgac gccgggcggc gcacggtgcc gccgaccaat tttcaggacc tggtcgcttc
     6061 gtcggggctc cggctcggaa gcgccgcctc gctggcgatc gaccagtacc acgtgcacga
     6121 tgcgctcgag taccgcaccg gcggaaccgc gcgcaacacg cggggggcgc agaccgcgct
     6181 ggcgaccgcg gagcactata tcggggcgcg cctggagatg ctccggtcgg ggacgctcct
     6241 ccgcctgcag gcggccgtcc agagcacgga ggagtcgtat gcggcggcgt cggtgccgga
     6301 cggggacgag gccgaaccgc cgatcctcga tctgcacgcc tccggagtga cgacgacgat
     6361 cgcgggcgag gttgtgctgt cgctcggggc gtgcgaactg acgatcggag accgggtccg
     6421 gcacgtgatg aagcagcgga tcgacctgac gcagcagaac tggaatttcc tccccccgga
     6481 tgcggcgagc gacctgccca atccctatca gccggaattg aaccggctct atggatccta
     6541 ccacgggacg cggcggcgca ccgaccatgc cgcctacgcc tcgctgcgct ggcgggcggg
     6601 gccggtccac atggaatcgg gcgcgcgcat cgggcacttc ggggggctgg cccggaaaga
     6661 gtgggcggta ttccgccagg ccgccacgct cgacctggac gacaaggggg cgctgacttt
     6721 ctttgcgggg acgttcgcgg aggatccggc gcagcgcgtc ctcgaggcct accaggcgct
     6781 ggtccacacc gactacaacg ggctgcagcc ggtgcgcact gcgctcgtgt cgctgcagta
     6841 cacctgcgga ccggcgagcg cggccctgta ccggaaacaa atcgccgggc tgccggtgct
     6901 cacgcccgac ttccgccggg tcgctgagga cggagcggcg ggggagggat tcctgcggac
     6961 gccgtcgatc ggggaggcgg attttgccgg cggcgacatt acgctcgcgt tcgacagcct
     7021 cgccgggggg cggctcgccc tgaccgcctc ctacggatac accgctggga cgaagacgac
     7081 tgcgagcgtg acggcgccct ccgaattcaa tgcgccgcac cggtggtacg cgatggcgcg
     7141 ctaccgggtg agccgcgtgg tggcggtcgg ggcggaagcc gcggtccggt cgggatacgc
     7201 ctacaccctg ccgccaaaaa gcccggcggc cccggacggg gaccgcttga ccgtcgacta
     7261 ctaccgggcc gccctggcgc gggagaacgg cgcgcgcttc cccacgcacg cgactctgaa
     7321 tctgaatgcg gtgttcgact tcggcgcggc cgaggcctac tgcgccattg ccaacatcac
     7381 caatcgggcc aacccgatca tccggtccgc cgacggtttc atctacgacg ccgggatcct
     7441 gcccagcgcc ggcgtgaccg tgcggttctg aagcctgaag cccgcttttc ctacaaagag
     7501 ctacacgtac cgggaggcga cggcgagcac gaccagcaga aacgccagat tgatcgggat
     7561 cacccagtgg tagaacaggt ggccgaccct gccggtctcc tgccgggtcg ccgcgcggta
     7621 ccgcgaggcg tagatggggc tcatgaggcg cagccacaca aaactcacct tgtcgccgcg
     7681 gcgccggagg tagttataga tggccatggt cgcgatcaca tgccagacca caccgatcaa
     7741 ggcccccagg gtgaggacat aggtcagggc gctcatacgt gctcctttct gccggggcgg
     7801 gccatcaacc cgccgcgccc aaagaacggc gatacgccgg ccgcgaggac cggggccatg
     7861 aatacggcgc ccgcaggacc gaacccggcc tctcgaacgg gagtgagcgc gttccggttt
     7921 ttcacacaaa ctccacttcc tgctggtcgc ggaagaaaat gagggcgagg acgatggccg
     7981 agacaggcac gaggagaagg gccaacacgg acatcagcac tgtgacctcg cagatcccgg
     8041 cagccatcgt gacataggcg aagccgcgga tgtattcgcc gaagcgttcc ttggccccga
     8101 gcagtttgac cgcgatcaga atatccacga cgccgatcga gaccatggcc ccggtgaaaa
     8161 aggccacaaa cccgaccagg agcaccaccc gatcgaccgg ccagaaggcg gaggtcagga
     8221 tgccgaacgc cagcccgcag acctgaaacg cgatcgtcca ccagaccgag acgatgatca
     8281 ggaggtcgag atcgcggtag gcgtaacgct cgtgcaggag cgagcggaat ttcagcagcg
     8341 tgtagaccgc gatcgccgtg aacggaatca tgagaaaatc ggaggggccg acaaacggcc
     8401 ggcgaatgtt aaaaaactcg gcggctacgc ccgcttcgat aagaccgatc acgatggcga
     8461 cgggaaagag cacggcctgc acgatcgcaa gccaaccggc cagcttatac ctgttctctt
     8521 ccatacttcc aagaccccgc cttcaggatg cgtgaggttc tctgtctcct aaacaaaatc
     8581 cggttcgtat tccagaatgt cgccgggctg gcattcgagc tcgcggcaga tcgcctcgag
     8641 cgtgctgaaa cggataccct tcaccttgcc cgttttcaga agcgagaggt tggtgaccga
     8701 tatcccgatt cgggccgcca gttcggtgag cgacatcttc ttcttcacca gaatcaggtc
     8761 cagtttaatc tgtatagcca cggttggtct ctgcggttga cagtgcgaat tacataaagt
     8821 gttttatgtt acacataaaa tacgttaagt aaaacgaaaa gattcggaga tttccggatt
     8881 ttttttgttt ggagactatg ccccctctta agatactaac ccacaatatc ttagggctgc
     8941 ctccgacggc cggcgccgcg aaggctcgcg gctgatcgtt cgcgcggtcg atgcgcgagg
     9001 acggaaccgg gcaccggggg aggggttgtt aggtctgtcc cgcgaagggt ggagatggag
     9061 acttttttac agaatcacta tttgctctac ggagcattca ccgcttttgt gctcgggatg
     9121 ctggctctgg atctcggcgt ctttcaccgg cgcgaccacg tggtcagcat gcgggagtcg
     9181 tttgcctgga cggcggtgtg gatcgtgctg gcgctcgcgt tcatggcgtt cgtgtactac
     9241 cggtacgaga cggtctcgcc cgggcggggc actccggcgg cgctggagtt tctcaccggc
     9301 tacctcatcg agaagtcgct gagcatcgac aacgtcttcg tcttcctcct catcttctcg
     9361 tacttcaacg tggctccgat ataccagcac cgggtgttgt tctgggggat tctcggggcg
     9421 ctcgtgttcc gggcgttttt catcgccctg ggcgccctcc tgatcgcgaa attccacgcc
     9481 atcatctacc tcttcggcgc tttcctggtg ttcacgggga tcaagatggc ctgggcgaaa
     9541 gacaagcagt tccacccgga gcggaacccg gtgctgcgac tctttcggag gttcgtcggg
     9601 gtcacaaagg attaccgggg gaagcgcttt ttcgtcagag cggcgggccg ccggctggcg
     9661 acgcctttgt tcgttgtgct cctcctcgtg gagtccagcg acatcatatt cgcggtcgat
     9721 tcgataccgg cgattttcgc ggtgacgaag gatccgttct tggtgttcac ggccaacgtg
     9781 ttcgcgattc tcggattgcg ctcgctctac ttcgcgctgg cgggcgtgat gcggctgttc
     9841 caccacctcc actacggcct gtcggtcatc ctcgtctttg tcgggatcaa aatgatcctc
     9901 tcggatatct acaaggttcc gatcggcatc tctctggggg cggtgggact gatcatcgcg
     9961 gtgtcgatcg ccgcttcgct ggtttggccg aaacgcgcgg tcgcgcccgg cgatccgccg
    10021 gccctgcccg agattcccgc gtaagtcgga aagttccggc cggtccggga ggtcagcccc
    10081 cgaggcccgt ggagcgcggg ggcgcagggt cggccggggg ctgcccggtg agcttgcggc
    10141 gcagccggcg cagcaggttg cgcgcccaca cgaactcaag ggagaggatg ccgaggccgg
    10201 ccggaatgac gagaaacgcc gggccgggca gcaccaggag agctacgccg agcgcgagca
    10261 ccgtgaaacc gatcacggcg acgaccagcc ttcgcacttg tcttatcgtt ccggcaatca
    10321 tcgcttcccc cggtcaccgg tacgccggga gcagtcccgg gtcggtgaga ggcggggcgg
    10381 ccggcgcccg ctcactgcga ttcgtcggtg accacgtggg cgacgtcgat cacggtgtcg
    10441 tcatcgcgca gctgcatgag ccgcacgccc tgggtgttgc ggccgatgac gctgatcccc
    10501 ttgaccgcgg tgcggcacgt cacgcccttg cgggtgatga ggatcagttc gtcctggtcg
    10561 aggacctcct tgatcgcgac cacctccccg ttgcgctcgc tgctcttgat gttgatgatg
    10621 cccttgccgc cgcggttggt gatgcggtac tcgtcgaccg aggtccgttt gccgtagccg
    10681 ttctcgcaga ccgtgaggat ggtgctgtcg cgtttgacga ccaccatccc gatggtgtag
    10741 tctttcttgc cgagggtgat ccccttgacg ccgtaggcgg tgcggcccat ggcgcggact
    10801 ttctcctctt tgaagcggat cgccatcccc ttgcgggtgg ccaggatgat gtcgtaggtg
    10861 ccgtcgctga gacaggcctc gatgagttca tcctcgaccg ggagggtgat ggcattgacg
    10921 ccggtgacgc gcgggtggga gaatgagtcg agagcgacct tcttgaccac cccctggcgc
    10981 gtggcgaaga cgacgaagcg gtcgggggag aagtcgcgga cgcgggtgaa ggcggtgatg
    11041 ttctcgtctt tctccatctg gacgaggttg acgatcggct tgcccttggc cagccgcccg
    11101 cccaccggga tctcgtgcac tttcacccag tagcagcggc ccttgttgga gaagaacagg
    11161 acgtagtcgt gggtggaggc gacgaagagg tgttcggccc agtcgtcctc tttggtttcg
    11221 atgccgcgga cgcccatgcc gccgcgctgc tggcgccggt agccgctcac cgagaggcgc
    11281 ttgacgtagc cctggtggga gatggtgatg accatttcct cttcggcgat gaggtcctcg
    11341 acgctgagct cctcggccgc gtcctggatc tcggtgcgcc gggcgtcgcc gtacttctcc
    11401 gccaggccgc gcgtttcatc cttgacgatc tgcatgcgga gcgcctggga ctccagaatg
    11461 gcgcggtact cggagatctt ctggatcagc tcgcggtact cgtcctcgat cttctgccgc
    11521 tcgaggccgg tgaggcgctg gaggcgcatc tcgaggatcg cggtcgcctg gacgtccgac
    11581 aggccgaact tcttcatcag cccggcgtgc gcggtcggcg tgtctttcga cttccggatg
    11641 agggcgatga cttcgtcgat gtggtccagc gcgatgcggt agccctcgag gatgtgggcc
    11701 cgctcctcgg ccttgcgcag gtcgtactgg gtgcgccgca cgaccacttc gtggcggtgg
    11761 tcgacaaacg cccggatgag ctgtttgagg ctcatctgtt tcggcacgcc gtggtcgagc
    11821 ccgagcatgt tgaccgagaa ggtggtctgc atggtcgtgt gggtgaagag ctggttgagg
    11881 acgacgtcgg gcggaatgtc gcgcttgagc tcgatgaaaa tgcgcatgcc gtcgcggtcg
    11941 gactcgtcgc ggaggtcggc gatgccgtcg atcttcttgt cgcgcacaag ctcggcgatc
    12001 ttctccagca ggttggtctt gttgacctgg aaggggattt cggagacgat gatggcgtcc
    12061 ttgccgttct tcatgcgctc gatggcggcg cgggcgcgca cgtacacgtg ccccttgccg
    12121 gtgcggtagg cctcgcggat gccgtcgcgg ccgttgatga tcccgccggt ggggaaatcg
    12181 gggccgggga caatctccat gaggtcgtcg ttcgtacact cgggatcgtc gatcacgcgg
    12241 atgatggcct cggcgatctc gttgaggttg tgcggcggaa tgttggtcgc catgcctacg
    12301 gcgatgccgg aggtgccgtt gcacaggaga ttggggaact tggccggcag gaccaccggc
    12361 tcctcccgcg tgctgtcgta gttgggcatg aagtcgacgg tctctttttt cagatcgtcg
    12421 agcatctcca tcgccatcgg tgtcaggcgg gcttccgtgt accgcatggc cgccgcgccg
    12481 tcgccgtcga tcgagccaaa gtttccctgg ccgtccacca gcggatagcg catgttgaaa
    12541 tcctgcgcca tgcgcacgag cgtcgggtac accacctgct cgccgtgggg atggtaattt
    12601 cccgaggtgt cgccgcagat cttggcgcac ttacggtgcg gccgtccggg cgccaaattc
    12661 agatcgttca tcgccaccag gatgcgtcgg ttcgacggct taagcccgtc gcggatgtcg
    12721 ggcagcgccc gattggtgat caccgacatc gagtaatcca ggtaggagtg cttcatctcc
    12781 tcttcgagga agatcgtctc tatcttttgc cgctccattg tcatggccgc cgaaggtccc
    12841 ttctatcttg cagtcgtaaa gtccacgata cgctgacgat aagctaacca ttatatgcgc
    12901 gaatagcaat aaaaaaccgc cgaaagcctg gcattatccg attgtgcgac aatgtattag
    12961 cggccgcttc ccgaacggcg gacgggtggc ggcatcgggc ttccgccggc cgcgctgccc
    13021 gacgtgcggc tagccgaggc cgatcgctta tgacgccgcc tgggaatgtc gatcaatgga
    13081 ctatggcgac acgtccgcag tttgaccaag cgtccataag aatcaccgct cccgtgtccc
    13141 gcgcgccgct ggggccgctg ctaaggagac cgccggtgct cgtttatgct gtggtcaccg
    13201 catatcgaac ctggctggtc gcgctgctgc tggcggccgt gatagtggcg gcggtgaagc
    13261 cggcggtgga agtcggcgtc cggcaggttc tccccccggt cagacagagc aagatgctcg
    13321 ggctggtgaa gcgggagaag cctcgtccgg cggctctcgc ggtcacgcgc ctgctcatgc
    13381 tggcggcctg ggcgggcgtc ggcggggtcg tgtttctccg gtggcgccgc gaattgccgg
    13441 accggctcgc cgcggcggag gcatgtgccc gccggtggga gcacgaggcc gagcgtctgg
    13501 ccgaaacggc gcccgatcgc agccggcggc tgtatcgggc cgcgctggtg ctgacgctcg
    13561 atcgggaggc ccggcggcgg gtgcaggccc ggctgtgcgc tccgggcgga gcggccgatg
    13621 ccgccgcgac atgcgccgcg accccggcgg acaccttggc cccgcccacc cgggcgggcg
    13681 cggaagggcc ggcgccgccg ggtctcgacc gccgctaccg gttggaggag aaactcggcc
    13741 aaggggcgat gggcatagtc tatcgcggcc atgaccaggt gctcgaccgc ccggtggcgc
    13801 tcaagcggct cgccgcgcgg ctcgtcggcg agtcggtcta cagcgagcgc ttccgccagg
    13861 aggcgcgggc gctggcccgg ctgtcgcacc cgcacatcgt gcaggtgttc gacctcattg
    13921 cggtcggcga agagatctgg atggtgcttg agtacctcga cgggggttcg ctcgcggatt
    13981 atctgaaaac gcacgggcgc ctcgagccgg ccgaggcggc gcggatgatc tgcgggatcg
    14041 ccgacggatt ggcggtagcg caccgagagg gaatcatcca ccgcgatatc aagccggcca
    14101 acatcctgct gaccgccgac cggcgggcca agctctcgga tttcggcatc gccaagctgg
    14161 ctcgcgaggc gacgctgaca caagcgggat cgaccctcgg ctcacccgcc tacatgagcc
    14221 ccgagcagtg cagcggcgag gggctcgacg agcggtccga catctactcc ctcggcatca
    14281 ccctgtacga gctgctggcg gggcggccgc cgttcgccgg agagacgagc ggcatactcg
    14341 cgcagcacct ggtgggcgac ccggcgccgc tggcgcagtg ggcgccgggc gtaccggaac
    14401 cgctcgatcg actggtgcgg ctcatgttgg cgaagcggcg cgaggatcgc ccgcagatga
    14461 tggccgaagt cgcggcggcg ctggacggct ggaccgaaag gaccgctccc gtgcccggcg
    14521 cctgacccga ggcccgtcgg cacgcgcggc tttggcccct cgctcgaaca gcgccctcgg
    14581 tcttgacaaa atggaccgcg ccggttatag ttacggcagg tcccataagt ccttttccct
    14641 cgattgacac cgacgattcc ggcagggcta gtttgacgca ggaggagtaa tatgaaagca
    14701 tgcgtgcggg ccgccctcct ggcgggggtc gcggcgcttg cgagcacggc cggggcgagc
    14761 cggctcacgg atctcacggg ctggtggagg gtgatcgagg acaacgggca tttcatccgg
    14821 ttggtgcagc agggggtcga tgtcactata ctcgacgggg acgggacact catcgccccg
    14881 ggcgtctttg ccaacgatac gctgtgggcc tacccgacgc ctgccgacac ggtgattctc
    14941 gtgtaccatg gggacacgct gagcggtctg aacgaggaag ggaatcccat gacgatcgtc
    15001 aggtacccgg tggatctcaa cgggtggtgg gcgaatgtcg acggagggag ggaaattccg
    15061 aggtttgtcc acgagggctc ggcggtgcag ctctggatgg ataccgatac ctcgacctgg
    15121 tttgtcgccg acggcacgtt tgcgaatgat acggttctgc ttgacctgcc gccggagctg
    15181 ggtggcgcgc tgaccctgat ctaccgggcg gacacgcttg gcggcatggg gggaccggac
    15241 gcggattcca tcattctggc gcgctcgccc tacggaaagt ggatgccgat tcggtgcgga
    15301 acagttgcca ttgggggaga tttcgaagac tggccataca tgtatctcat ggctcaggac
    15361 aggcgcgatg acgggaccag caccccggcg gccgaactgg atcgggtcta tctctgccat
    15421 gattcggact actactactt ccggatagaa tgtgtcggcg acgccgcctt tccgcacagc
    15481 gggacgctct gggaccgcta ttcgattgtc tggtatatta gcggtaccga cagtacgtgc
    15541 agcatcagaa tcgagtccac ctgggaaatg tacttctgga acaacatgac gcagcaggaa
    15601 acgatgctcg aaccgctggc ggtgtcgggt cgcgtcatcg agggaaggat accgcttgag
    15661 ctgctcggtc gccttgcgga agaagcggaa gtgtccgtca ggtcggcgta ctacgactgg
    15721 gatttcgggt ggctgtcgta cgatgagatt caattcaatg cggcccggca agcgtgcggg
    15781 gccgatcccg cgcggctgac cccgcagccg cagtatgctt tcctgtcaaa cgccgtgaca
    15841 cccgtcatgg atacgctcgt tatcggcaac ctgccggacc gcgccgccgg cgacattcgg
    15901 ctgagctctc tgcgggtcaa cggagctgtc cagccgacgg tctgcgcggg gcacttgaaa
    15961 tgcccggggt tcgcgggcag cgtggtgaca gcgctttttc ccgcccggac gtttctccag
    16021 gggtacggac tggtctggga cacggcccgg gcgccataca cggtgacggg ggaattccgg
    16081 gacggtgcgc cggtgaatat cgaaggcgag gttacgataa tcggccaccg gtcgggggac
    16141 gtgaacgggg atgggacggc aagcgtggcg gatgtgacgt tcctggtcgg gtacgttttt
    16201 cgcggcggcc gggcgccgca gccggtcgag ttgggggatg tggactgcga cgggggaatt
    16261 acggtcaccg atctcagcag cctggttgca tatctcctgc ggggcggtcc gcccccgtgc
    16321 gctcgctagc ggatcatgcc gttcgaatca caggggccgc cttcggcggc cctttgtcgg
    16381 agaggggcaa acgggcgcgc cccggcgccg aaaacccggc ccccgaacca attcaacctt
    16441 gccggttaca aagggcatga tatattcccc gcacaagaag gacggaaagg tggtatgggt
    16501 acgatggttc gccgcatcct gttgtctgcg cttctgggag ccgtgctcgc cgccgcagcg
    16561 cggccggccg ctaagttcga tctgaaagga ctcgccggtt cgtgggaagg cgaggggatg
    16621 ttcctcatgc cggtcaccga tcttgaggtc gaggtggaag gggaagggga gttcgtctac
    16681 gatgcgaagc gggacctggt caagaccacg atgtcggggt cgaagttcct tgtcccgtac
    16741 tccgattccg ggctgctccg gtacgacctc gcaaccgact cggtgtcgtg ggaggtgtgg
    16801 gacaactggg ggcagcattc gatgtactac ggccaatggc gcgacggcgt gatcgcggcc
    16861 gaccggctgt ggaagagccg caactaccag gtgaccgtga ctttccccca ccgggacacg
    16921 ctcaattttc acctgaccgc caccgatccg cagacgcagt tgaaaacgac ccgggcgcgg
    16981 ttcagtctct ggcgggtaaa gtgagcgatg gcggcctggg cgacgactgt tgatgcgctg
    17041 gtgcgcaatt tcctgtccgc cctcaacgcc tcggtgccgt cgatgcgggc ggcccgcatt
    17101 ctggacgacg acttgaccgg attcgacgac tgggagcggc tgtgcgatac gctctaccgc
    17161 ctcctggtga tcgaaccgat ccgcgcctcc ctgcccaaac ccgcacaggc cgtctttgac
    17221 ctcccctcgt atgaaaccca gtacgaatcg ctggaggatt tcagtctcat ccaggtgacg
    17281 ccgccgggga aagcggcgcc gccgcggcaa atcgccggaa cggccgtgtt cttccgcttt
    17341 ctgcccggca acgaacagac cgggcggttc gatctggtgg agaccgtgag agtgaacgag
    17401 gggctgacga tcgaggagac ctcgtgggag acggccaggg cggaggaagt gtatttcacc
    17461 tgcctcgtcc cggagcgcca gggatggcgg ctgctcgatc acgtgcaggt cgagatggag
    17521 tagtcgccgg gccgaagggg ggtccgcacg ccccgcggag attttcgaca gttgccgggc
    17581 ccgagaattg cggcgcgccc atcaccccga ccggtgcaat cgccgcccgc atccggcggc
    17641 ggttctactc ccgatagtac cagtgccacg attcccagta gtcgtagtcg gacttctcca
    17701 ccggtttcgg gtaggattcg acaaagccga aatcggcggc gtgccgggtg agccacttgt
    17761 aggcgtcggt atggacgaaa cgggcctcgc tcggcacgag atcgagcgcg cgcgccgtgt
    17821 ggtgctggga gaagccgggc ggagcgacga aacgcacgac ctggtggaag gtctgcccct
    17881 cggccatgcg gcgccggatc acccgcttct gataggccgg ggaacggtac ccggagtcga
    17941 cgcggaagcg gatgccggct ttgcgggccg cctcggccat gcggacaaac gcctcgcggg
    18001 tggcgcgggt gacgtagatc cggctgccgt tgtcgcagag atctttcggt agggccacga
    18061 gcgtgtcggc cgccgcccgc tcatggggcg gaacatagcg ctcgcaccac cgctccggca
    18121 cgtcgtaggt gacggcgtcg atggtgacgg aatggttgca agggcgctcg gccttggccg
    18181 accggtcggc ctgcggggcg ccggacgcga agcagagcgc cgccgcggcc gtcaacacgc
    18241 ctcgtctgac ccggacgttc ctcacattac cctacgtagc ggaggccgga gttcgtaccg
    18301 gtgagaatct gacggtcgct ttccagtccg gccgccttca cctctccgat gaacaggatg
    18361 tggttgccgg tctcgaccga gttgacgatg gcgcagtcga catagccgag gatcccgtgg
    18421 agcaccggcg tgccggtggc gggggaattg ccgagggtgc gggctttgag cttgtcgtag
    18481 ccggcctcgg ccgggccgta gaacgacttg gcgaccgctt cctgtccctc gccgatgaga
    18541 ttgacgacga acgcctggtg ctcccgcagc aacgggatcg actgccgttt cttatgcacc
    18601 gcgatcgcga ccagcggcgg atcggaactg acctgggtca cccaggaggc ggtgaaggcg
    18661 tttccgtttt cccccttccc cattgtgacc acgtagacgc cgtattcgaa cttccggagg
    18721 acttttttga tgtcgtcggc catggcgatt accctttcgc aggtggcgtg ttgctttcga
    18781 tcggccagca taccgtttcc ggcgcgggcc gtcaattact ttccctgtaa ccggccggcc
    18841 agcctcatgg ttcgaagggg tgatcgcgca tgaagtcggg gcggtagtgc tcgctggagc
    18901 cgagttcctg gacccagccg cgggtgaacc ccaggcgttc catcgcttcg agcacctcat
    18961 cgtactcgtc ccggaggacg cggcggccga gggtgggatg gcgggccacg gccggcgtcg
    19021 gccagtactg cgacatgagc gagaggtggc agtcgggcga aaaacactcg gcgatgcagc
    19081 ggagaacctc gatcgagttt tgcacctgac cggggagcac gaggtggcga atgaccaacc
    19141 cgcgccggat caagccgtcg gcgccgagct cgatctcggc gcccatctgc cggaacatct
    19201 ccccgatcgc ggccgccgcg atctcggggt agcggggggc ctgcgaaaac tcgcgcgcga
    19261 gccgctcatc catgtacttg aaatcgggca ggtagacatc gatgatcccc tccagccgcc
    19321 ggatcgcctc gacccggtcg tagccgttgg tgttgcacac gtacaccggc cggcggccgc
    19381 gggcggcgag cacgtcgatg atgcggagca tctgcgggta gtagtgcgag ggggagacga
    19441 agccgaccga agggcagccc cggtcgagat gctcctcgac ccggtcggcc agccggtcgt
    19501 agctcatggc ggtttcgatg atcggttcgt gcgtgcggct gatgtcgtag ttctggcagt
    19561 agacacactg gaggttgcag cgggtgaaga agatgttgca gatgccgtgg gggccggaga
    19621 tgaccggctc ctcgccccgg tgggcgcaga tcgaaccgat agacaggtcg gcgccgctgc
    19681 ggcaccagcc gagcgggctg gcggtgcggt cggcgccgca gccccggggg cagcaggcgc
    19741 agcgctccag gttcggttcg aacggtcggc gatccgatga actcatgggg ccaatatagg
    19801 acaggttctc tccccttgcc agactcatcc ggcggaagac aaaaaaaagg gcaggccgca
    19861 ggcctgccct tgcactctca ctgagccgtc actggcacac cagcgccggg ccccctcgga
    19921 agaggaaggc gatcatgtag gtgacgtcgc cgacggtgat gccgacctgg ccgtcaacat
    19981 ccgccgccgc catgttggcg ggggagggac cgccgcggaa catgaaggcg ataaagaagg
    20041 tcaaatcgcc gacagtgaaa ctgccgtccc cggtaacgtc gccgcacagg taatcgcagg
    20101 cgtcgccgac gccgtcgagg tcgccgtcgg cctggtccgg gttagcgatc tcgggacagt
    20161 tgtcacaggc gtcgccgacg gcatcctgat cgccgtcgac ctgcgtcgga tttgtgacag
    20221 tcgggcagtt gtcgcaggcg tcgccgcgga cgtcgccgtc agaattcgtc tgactggtgt
    20281 tgggcaccgc cgcgcagttg tcgcaggcat ccccgacacc gtcgctgtca gcgtcgttct
    20341 gagtggcgtt ggcgatggca gggcaattgt cgcaggcgtt gccgaaagcg tcggtgtcgc
    20401 tgttggcctg gtccggattg ggggtggtca cgcagttgtc gcaggcgtcc cccttgccgt
    20461 cgctgtcggc gtcggtctgg atctggttgg cgacggtcgg acagttgtcg caggcgtcgc
    20521 cgaaagtatc gctgtcggag ttggcctggg tggggttctg gaccgacggg cagttgtcgc
    20581 aggcgtcccc cttaccgtcg ccgtcggcat cggtctggcc ggggttggcg accgtggggc
    20641 agttgtccga cccggtgggg atcccgtcgt tatcgggatc gggatcggca gtcgggaaga
    20701 caatgtagtc cacccagccg cagtcgctgc cgaccgactg tgatccgtcc ttcgtgtagg
    20761 tccagcggaa cgtatggcta ccggccgtgg tggcgtagga cgcctcggtc caggcgacgg
    20821 tgccggacca ccggtccttg cggacgccgt cgagccagaa ttccaggtag tcccagcccg
    20881 actccgacga gaccttgtag cggaaagtca ccgtgccggc ctcgaggttg ctcagcgtga
    20941 cctgcatggt ggaggtctgg ctgtgcgaaa tggtccccga cttggccgag taggtgccct
    21001 cgtagggcgc cgaagtcgtc gttacccacg cagcgttgcc gctctgcgtc cacgggaagg
    21061 cggcgaaatt gccggtctcg aagtcttcga tcgtctgccc cacggtcaac gaaaacagca
    21121 cgctgtcgct gtagccgccg tcggcggtga ggtgtacaac gaaatcgacc gggtgctcga
    21181 gcgggcagga gggggagacg ctgaactggt aggcggtgag cgacgtgccg gtgccgccca
    21241 gggcggtgat ggtcgggaac atggcgctgt tctgcgtgat cgtgatgtag ctgtcggcgg
    21301 tcgccagcgt cccggccaga ttaacggcgt tgccggcgcc ctcgtttttc agtgtgatgc
    21361 tcatggccgc cgattccccc ggatccagcg cacccgccgt ctggctcacg aacgtcagaa
    21421 ccggcgcgtt cggcagcggc tggagctgta cgttctgcgt gaccgtgccg gcgtaggaag
    21481 cgacggtgat gccgaaaaca gtgtccgggt agtagccggg cgagctgtag acgaagttcc
    21541 aggtaccggg agcgatcatg cggtggtagt tgcccagcac gggatcggtg tagacctggg
    21601 agctgtcggc ctgggtgtcg tggctgagca caaagatcgt ggccgccagc ggcaggccgg
    21661 tgttggcgtc ggtgacgatc ccgcgcacgc cccggagggc attgcgcagg tagttgaaca
    21721 gcgagacccg caggtagccc caccaggtcg ggagctggcc ggcggggagc agcttggtct
    21781 cggagatctc gatggtgact tcgcggcagc cgtgccagta gttcatgaaa tcctggcggc
    21841 cgccggtgat cgtgtaccag tcatacccgt tggtgatgcc gtcgttgaac ccgtccatgt
    21901 agccccccga gctgtagtac tgggcggagt cggcgaacac gtgtgagacg gtctgccacc
    21961 aggcgtcgtc gggatgccgg cgcgaccagg tgtcccaggg gtagttgacc acttcggccc
    22021 cgccgtggaa gttggcggag atgataaagt ggcgggcctg ggcgaacgcg atcatggcgt
    22081 tggtttccgg ctgataggcg ttgccgtccg ggtgcggccc gtcgtcggcg tcggggaagt
    22141 tccggttgag gtcgacgccg ttggcgttgt agcgggtcgc cccgtacact gtcgaattgc
    22201 cgccggcgta ggtcccgtcg gggttgtgca gcgggttgat ccagatctcg agattgtcga
    22261 ccatgctggt gatctgagga ttgacgccgt aggcgctcag gagggagtcg atcatgcgca
    22321 gcatcaggac gtacccggtg gtctcgtcgc cgtgcatcga cgacagatac agcacttccg
    22381 gctcgtcctc gtcgaccgcg acgttgtcgg agatcttggc ggccaacagg gcgcggccct
    22441 cggtcgtcgt gccgatggtc acgatttggc agaggcccgg gtaggttgcg gcaaactggt
    22501 tcatcatggc cacgtaggca tcatacgtgg ggtacacatc ccaggccctt gcatcgcgga
    22561 gatcggacgt catttcgggg acgatcagcg tgctcgggcg cgggagttcc tcgaaggcat
    22621 acccgaggcg ctccagcgca gccagctgct cgtcggtggc gtacgccagc acgacgccgt
    22681 cctgcacgtt gtcgatcgag acgacctcgg agagctcggc cagtgccttc ggggaatcgg
    22741 ccttgagccg caggtagtgc tcgttgacgg cgacgacggt cgacgcgcag aggacaagca
    22801 aaagcaggac ggttgcacag cgaagagaac gcatcataac tgctttcctt tggttcggcg
    22861 gagcctatgt aagtattttc gaacagtacc tccacaatat aaaccaaaaa accgggcgac
    22921 aaaggggttt ttgccgcccg gtaaggtcag tcggtgcaat cagttgccgc aggtcggcgg
    22981 cgaccccccg cggaagagaa aattgatcaa ctgcgtgaga tccggcagcg cgatgtcgcc
    23041 cgagccgtcg aaatcgcaag ccgcgggatc cggcggcgcc ggcccgcccc ggaagaggaa
    23101 gttgatgaag aaagtcaggt cggccacgct cggccccgcc ccgtcgccgt tgatgtcgcc
    23161 gcagatccag gcaggctgga gcgtcaccgt cagctccgcc ggaaccgtct ccgccgggtg
    23221 attcggatcg ttgcagctca ccacgagggc ggcctggtag aggccggaat ccagacccgc
    23281 cgagcggaac atcaggccca cggtatcggt acctccggcc gccaccgttc ccgaaaagcg
    23341 gctgaggccc agccattggt agggctggac gaaccggatg cagagggagt ctttcaggta
    23401 gggcgtcagg tacgcaacct ccaggccgtc cgtgccgtcc gggctctcga tccctatcgt
    23461 gccgccggcc gcatcgaacc cggcggcgac gctcaggtac tggaagcgga tggaaccgtc
    23521 cggttcgagg ataacctccg ccgtgatcac gtcgccgggg ttcgccagat attcgggata
    23581 ggcggtgaac tgaatgacgc aacggccgcc gctcgtatcg aggtagacgt gggccgcggc
    23641 ggcattgttc acatcatccg ggttcaggtc gtcccacagc caggcgagga gggcgttggg
    23701 gagagtgtcg ttggggatgt gtttcttgaa gcgggagttc atcgctccgg gggcgaaccc
    23761 gatgatcccg ttcgacccga tgtagatctc ggtgtaggcg ctgtcataga acgggaaggg
    23821 gaagccgatg gcataggggc ctgcgaagtt atcgtcgttg agggcgctga tgacatcggt
    23881 gccggcggcc gagacgtcga tccattcaaa ggccggtccc ccggattcgt cggagtcgat
    23941 ccagaagtag ccgtggccgt ccggtccgcc ggcgctcttt tccaccgccg gcccgctccg
    24001 cggatcgtcg gcgcccttgt ggtcggcgta atcctcgaaa ccctccgcga gccgccggtg
    24061 cgcgggtgcg agttcccccg acgcgagcag ttcgcccagg cgggtgttcc ccttggcggc
    24121 gaccgggaca accgagagcg aataggtgag aacgccgagg ccggtgttgg cgatgagcag
    24181 ccggtccgcg gcggaatccc cctgcactag gcccgcgctg aatccggagg gcgccacggc
    24241 cagcgcggga gcgtagtcga cgctgatgtt gtcgacgaac cagaagtcgg aatcggcgcc
    24301 ggaaccgaac gagcggaagc ggacctggaa accgccgtgc agcgcgtcgg ccggcagacc
    24361 gaaagtcacg cgctcgaagc tcgacatggg cgcgcccgac ccgaggtggc gggcgacgag
    24421 aacccaggag ccggagttgt tcttgtactc cacccagaga tcgtcgcccg catcgggcgg
    24481 ctcggacgaa ccgccgcgct cgtagtagta ggacaggacg acgtcgctgc gccccgacag
    24541 gtcaatcggc tgggaggtca gcgtgtcgac gccgccgtcg agcgccgccg cgtagggggc
    24601 cgagggcggg aaaagcgccg ccgcgctaac cgccgcgccg acaatgcttg tccaccggga
    24661 gacgatgacc gtatcgaccg ggaagggttc gtaccacgga aatccgccgg gcgggccggc
    24721 cgagaacgaa accagccgac tgtcgtcgaa catgagcggg tccccggcgg agacggcgcg
    24781 cacacggcag gagtcgacgg cgttcatcgg ggcgtcggcc ggcacctcga ccttgaccac
    24841 aaaccgggcg gaatcgccgg cggcgatcat gccgctgccg ccgaccgggg ccaccccggc
    24901 gtcatcccag aacaacacgg gccacgcgcc gcccgagtcg gcgaagctgt actggtcggc
    24961 ctgcgacccc cggttggcga tctgcagcgt gtactgcatg gtgtcgccgg ccgggccgta
    25021 ctggctggcg aaggccgggt ccagagagag cttgaagggc tggggggggc cgacgtagag
    25081 gtcgtcgacg aaccagtcgt cgtaggcgcc cgccgtcccg atactcctca ggcgcaggcg
    25141 gaacgcgctg tgataagcgg ccggcggaag cgggagttcc acctcggaga aagtcgtcat
    25201 gtcggggcca gaaccgaggt gttgctgcag gagctgccac acgccggcgc tgtcgcggta
    25261 ctcgacgaag aggtcgtcgt tggcctcggg ggagtcgccg ccgccggtgc gctcgtagaa
    25321 atagcgcacg atcagattcc cctggcccgc gagattgatc acctgcgaca tgaccgtgtc
    25381 ggctcccgcc ggggcgccgt cgaggttgag gctgtacggt tcggagggtt cggcgagcgc
    25441 gacggcgttg gcctcggccc cggtggtctg gacccagtgc gcggcgctca gcgcggccgc
    25501 cgggaactcg tcctcgaacg gaatcgccag cggctcgccg tcggagacgg tgcggagccg
    25561 gctggccgcg gtgtagaccg gatcgccgac ggacgtggcc gtgacaaggg ccgtgtcggc
    25621 ggccccgtac accgagggcg gcacgatgac gcggaccgtg aacgcgaaaa cggagtcggc
    25681 cgccagcggg ccggtggaca aaagcggcgt cgtcccggcg gcgtcgaaga tttcggtagt
    25741 ccaggcgttg cccgacaccg cgagggcgta ggagtccgtg tacacgccgg tgttcacaat
    25801 ctgcagggcg taccagactg aatcggcaac cgggccggag agtgtatcgg cggcgggcgc
    25861 cagggtcagg ccgtagtcga tgacgcggaa gctgaaactg tcggtggtgt ccgccttgcc
    25921 gtcgctgtcg accgccagca ggcggtagct gacgactgtg ccgggagcct gcgcgggaat
    25981 gtagccgaca aactcatcgg ggttgccggt cggggtgagc gtatcctcgt agttggccgc
    26041 cccgatttca tagtagagca ggagcgagtc ggggtccaga ggaacgtcgc tggtgatggc
    26101 ggcgaggacg gcgtagtcgt tgaccgtgtc gcgggtgtcg gggagcggat cgtgggtgat
    26161 ggagatgccg gcgctctccc ggccgcccag actgagcgca cccgtgttgt cggggtccag
    26221 ccagtcgcgc aggcgggtgg cggcggtcga cccgtagctc cacgacttgg cgaacttccc
    26281 gtaccagtcg gaggtgatgc tcgcgcagga agcgtagccg ccgtggagct ggccgatgat
    26341 ctgtccctgg cggttgaaga tcggcgaccc ggaggacccg ggctcggtcg tgccgtcctc
    26401 ccactggccg atccgccagt gcgaatcgtc gccgggcacc gcggtcccca ggtagctcgt
    26461 ggaggtcacc gcgttgtagt cgaaagtgat cttcttgatg tcgcccgagg ggtggtggat
    26521 ggcgataacg ctgtcggcgg cggcgttgac ggcgttccag ccggcgtagt agacgttgta
    26581 ggaatccggc ggctgctcgg acagctccag gagcgcgaag tctgagtagg cgagattcgc
    26641 ccggagggtg gaacccgaca ccgtcatgta ggtggggccg tcgacgttgc tgcaggccgg
    26701 gctctcgtag ttgaaaatga acacccaggt gtcggagccg ccgaggcagt ggttggcggt
    26761 gaggaaatag ggggtgaggt cctgccgcac gttgttgatc atcgcgcccg aacagaggcg
    26821 gaagccgccg ccggtcgtga tcagggccac cgagcggatg aggggatcaa aggccgccca
    26881 ctcggggcag cggacgttgt tgttgcaggc gcccgattcg ccgaaatcct tgagaaaatc
    26941 ccgcgcgaac agattgcggt aggcgtggac gatgcgcgag acccgcagtt ccggctgcgc
    27001 gttcagcccg gccggctccc agtactccag cacgctcacg tcgccgcgca ccggctgagt
    27061 ggcgaaggag ccgtagtcct tgttgttacg gctcgtgaag gcgccgatga cgtgggagcg
    27121 gtcggcgttg tagatgaaca gccgggcgcc gtcgggcagc cagaaccggt cgaagatcag
    27181 gttgatcgac gcggcgccgg ggcattcgat ctgcagccgc cacagccggc tgccgtcggc
    27241 cagcacctcc cacgtgcccg cgttgtccag accgagccgg acatcgaacg ggtagccgaa
    27301 tcggaaaggc acatctttgg gcgtccgcgc gtcctcctcg agaagcgcgg ccacgtcgat
    27361 cggcgcggtg acgtagcggt cgattgcggc gcccaccgcc ttgccgaaac tcggcggcgt
    27421 gcccccttcg ctgacctgcg cggccacggt taccgcgccc gacagcgcgg tcgcggtgac
    27481 gaccagcatg aacctcagga acctcattgc gcaccccgca ggctgatggt tggtggaacg
    27541 gttggtgagt cgtggggaga agccgtcgac cgtcggccgc gccgggatcg gtcccgcccg
    27601 agaaacgatg gcggccgcgg cgggcggcgg tgcggcggat ggtggctgac ggtttggcac
    27661 tatcggcgat tccccatggg ctgtgtcgcc ggaggcgggc ccgcgcggac ggggcggaac
    27721 cgggactgtc ggcgccggcc gcgggcggtc cggatggcga cttcagaaat ccatatagtc
    27781 aattaagcgg cggacccgcg ggcggtcaag ggaattcatg ataatgtggg tagaatttcg
    27841 ctacgataga ccgccgggcg ggcaaagaaa acggccgccc gcaggggcgg ccgcttattc
    27901 atccgacgcc atccgcgccg aactcagctg cgcgcttcga ccatccggta aatccggctc
    27961 tccagctgga ggagctggcc ggccgtgccg gcaagcttca ccatgcccgc gagctcgctg
    28021 gtcgaagcga cttcctcgac ctgctcggcc acgtaccact ggaggaactg ctcggtggcg
    28081 atgtccccct cggcgcgggc cagcttgacc aggtcgtgga tgcgcttggt gatgtacttc
    28141 tcgtgttcga ccgcggcgtc gcagatctcc tgcgccgatt tccacgccgc cttgggagcc
    28201 tcgattcccg gcaccgtgac cggggcgccc tgctcgacga ggtacttggc gaacttcagg
    28261 gcgtgcttgt gctcctcttc ggcctgctta tagaaccact tcgcgaacac ggtcaggttc
    28321 atctcttcga acttgtaggc catggccagg tagatgtact cggagtcgag ttcggcgccg
    28381 atctgctcgt tcagagcctt ggccatcttc ttggtgatca tcatggtttc aagtcctcct
    28441 tttacagcgt atgatgttcg tcaattacct tggtaaacgg tcctgtattt tgatggttcc
    28501 ttcagatacc gggatttcgc gcaaaaagca agtctctggg gaggaaaagt ctgggaattg
    28561 aaaacagctt tcaataggtc cggtttcgga gggggagcgt cgcggggtgc atcgaccgcc
    28621 gctctcaggt catgcgccgg aggcgcgtgc agaatgcgcc gtcgagccgg ccgacctcgg
    28681 gataggtctt cacgaagccc cgttcggtca ccagttcggc ggggaagaag cgggcggcgc
    28741 tatcgatttc gaactggcgg ttgcggacga ggaactcctc gactacctgg tcgttttcgg
    28801 cccggatgat cgtgcacgtc gaatagacca tcacgccgcc gggtttgacg agggtggcgg
    28861 cgtggtcgat cattttcgcc tggatcttgg cgaggttggc gatgtcctca gcggatttct
    28921 gccagcggag atcggagttc ttgccggccg tgccccagcc ggagcagggg ggatcgagca
    28981 ggacgcggtc gaagggtccg ccgtggaaat cgagcatgtc cgagacgacc ggggcgatga
    29041 tcttgatgcc gaggcgctgg gcgttgtcga ccagcacctg gaggcgggcg tgggacttgt
    29101 caaccgccgt cacccgcccc ttgttgcgca tgcggatggc caggtacgtc gccttgcccc
    29161 ccggcgcggc ggtcagatcg atcacattgg tccggggttg cgggttgagc agacgggcgc
    29221 acaggccggc cgattcgtcc tgcacgaaca cctttcccgt gtcgatcagt tcgccctgga
    29281 gcggcagccc ggattcttcg atgtggatga attccggcag gtacttcccg taggagaact
    29341 cgatgttgtt gctctggagg atgttggcca cctcgtccgg cttggccttg aggaagttga
    29401 cccgataggt cacgtgcggc ggggcgttga acactcgcag cagttgctcc gtcttgtcga
    29461 atccgaactc ggtcaggcag tagcgcacga agtgttcggg gtagctgtag aagtcgccga
    29521 ggtacttggc cgggtcctcg tccttggaga cgaatctcac tttctgcggc tcgcggatcg
    29581 ccgagcgcat gacggcgttg acgaggttgg cgcgggaacg gtcggtcatg tagtgggcga
    29641 ggttgaccga ctccgacacc gcggccgccg ccgggatgcg gtcggtgaag tggagctggt
    29701 agaagccgag gcggaggatg ttggcgagtt tcagcggcag ttcgcgcgag ggccgggcga
    29761 gatagaactt gatttcgtgg tccagcctcc gccgcatctt ggtcgtgccg ttgacgagct
    29821 ggaggaggaa ccgcttgtcg atcgggcgca gcgcgcgccc gctcgtgacc tgctgcaccg
    29881 cgatgtcggt ttgctctccc tgttcgatca ggaccagcgc ctcgatggcg gcggcgcgga
    29941 cggcatcgaa acgctgcggc gatttgcgct caatttgctc cgccataacg ccctacaact
    30001 gcatggggtc gatccgctgc ccggcctcat cgatccggta cgcggcccgc cggtggaggc
    30061 ggtgcgcgag cgctttcgcc cgctccgccg tgaagagctc tcccagcact tcgtcggggc
    30121 ggacatatcc gccctcgccc acgccgagca ccatctcgac cgtgcggtcg gcgaccgtga
    30181 tcgcgtacac ggccggccgg atgtcgaccg ccgtcggccc attcttgccg gtgcgttcta
    30241 tctccacgcg ggctttgccc agcagctcct cggcccgcgg ccggacggcc gcgaggtcgg
    30301 cgtcgtcgtc cagcaccagc gtgtactcca cgcggttgag ctgggacgac agcgagggcg
    30361 ccttgccgag caccacgccc gcctcgtaca tcgccatccc ctccaccatc gccgcccgca
    30421 ccttgtcgag caggtagggc atgaaattgg tctcgagggt gaggtcgaaa tactccgcct
    30481 ccgaggtgaa cccgagcggg agcggcgggc cgaacgacag cttcattgtc gggtggaatc
    30541 cctggctgta ggcgatcggg atgcggctgc ggcggatggt ccgctcgatg aggcggaggt
    30601 tgtccaggtg cgacatgaag cgcaggcgct ccgtctttcc ccaccggatg cggaggcggt
    30661 tgcgggtggg ggccgcggtc gcgcgcgtcg ccactttctt cttcccccgc ccgaattgga
    30721 gctccggttc cggcagcggc gcggcggtct cgccgcggaa tctggggacg tactcccgca
    30781 actgcgtcga ggtgcggtgc cgttcctcct gaaggtgggc gagcgacggc cccttgcgga
    30841 tgtgcgacca ggggaggtcg gcggagaagg ggacggagcg gaggcgttcc tcgatctcga
    30901 tgccccggct gcggcagagg tcgaaccagc gctgcggatt gaaatcctcc gcccagccgt
    30961 cgaaccgccc gcccgcccgg tagacggcct cgatgacgtc ggccatctcg cgcccgcccc
    31021 gggagagcag cgccttgagc acggcttcct cggccgaacc gtgcttgaaa ttgacgtggc
    31081 ggctgcggac gtggcgcttg atgaatttca atttctcgtc gatctcggcc ggggtcgctg
    31141 tagcgtccca ctggaaggcc gtgtgcggtt cggcgacgaa gggcgagagg gtcacgttga
    31201 ccgtgcgctt gcccgggtag ttgcggctga ggtcgaacac ccgctcgatc atgcgggcga
    31261 tagccagcag atcctcctcc gtctcggtcg gcaggccgat catgaaatac agcttgagcg
    31321 tcgtcacgcc tttgctgaat gcgatctcgg cgctgtcgta gacggcggcg tccgggatat
    31381 ccttgcgaat gaacgcgcgc aggcgctcgg tgccggcctc gggggcgatc gtcagccccg
    31441 ccttgcgcac gagcttgagc gtgtcgagca actccggcgt gatgctgccg ggacggagcg
    31501 aggggacgct caccgcggcc cgcatcggct cgagcaggcg ggcgaccttc cgcgccaggt
    31561 cgtcgatgtc gggataatcg gaggtcgaca gcgagaggag agaaacggaa tcggcgccga
    31621 cgtggcgctc ctgcgtctcc acctgcgcga ggatctcgtg cggggggcgc agccgcaccg
    31681 gcttgtagat cgtccctgct tcgcagaacc ggcagccgcg cggacagccg cgcatgatct
    31741 ccacgctcag gtgctggtgc accgtctcga tcagcgggac cactggctgc gggggatagt
    31801 acgagggttt gagagcaggg acgacgcgtg cggcgatctc cgcgggcgcg aagtcgacga
    31861 ggggtcggcg ccggtcatca taaaagcgcg gcacgtacac cgactccacc tctctcacga
    31921 gccgagccag cttctccgcc cgggtagccc cgcgcatctg gtggagaatc gccagcatct
    31981 gggggagacc ctcctcgccg tcgccgatga aaaacagatc gacaaacggg gccatcggtt
    32041 ccgggttgta ggcggccgga ccgcccgcca gaatcagcgg ctgctcgtcg gtgcgttcgg
    32101 ccgcccgcag cggcaggccg gccagatcga tcatctgcag gacggtcgtg tagaccagct
    32161 cgtagctgag ggtgaagccg atggcgtcga aagcggccgc cggcgtgcgc gactcaagcg
    32221 aaaacagcgg aattccatgc ttccgcatga tctcctcggc gtcgcggtcg ggggcgaaga
    32281 cgcgctcccc cacaaaccgg tcgtcctggt tgaccacgtg gtagagcgtc tgaagcccga
    32341 ggtaggagtg cccgatttcg tatttgtcgg gaaacgcgag caggtaccgc aggcggccct
    32401 cgtgcgcctt gacaagcgag ccgggttccc cgccggcgta ccgccccggt ttgaccacgt
    32461 aggggaaaaa tctctcttcg aggagcctct tcatcgcgtc gtacgtgcta cgtaagcatt
    32521 caggtgcttc gggggtccca cttgcttcgg acacttccta tatagttgtc ggcagaatcc
    32581 ggcgcaagcc tgaaagtgcc ggatccacac cgccgcccag gggggacggc ccgggcggag
    32641 cgcacgccgc acacgtctca gggaaggcgc gggcggtcca gcgaggcgct ctcgagtttc
    32701 tcgattaccg ccttacggcc gtgcgcgatc actttgaatc cggtcgccga gaagcgcata
    32761 tcccgggtca gttcgcgtcg gtccagggcc gccagcgtgg cctcaaggcg atcactcgca
    32821 accggttgcg cgggggccgg cgcccccccg aggtgcgggc gcacgaagta tccaagcccg
    32881 aacaaaacca gcgccgccgc cacccgaagg gcgtagcggg caaaccgccc ccctcccgag
    32941 gcggccgact ccggggtggc cagcctgcgc cacacggtgt tctccaggcg gagccgctcc
    33001 agttcgctca ggccgccctc cccatcggag gtcagcgcgg agcagatgct ctgcaggcat
    33061 tcgaactcgg agcggcaggc ggcgcaggcg gccagatggt tcatcacggc cgggcggtcg
    33121 ctttcggaaa cttcgcccag caccagcgcc gggagcaggt cccggcattc ccggcaggtc
    33181 atatgctcat ctccttcatc ttgtcggcca gttgcgcgtt gaggtttgcc agaccccgaa
    33241 acaggtggac cttgaccgct ccctcggaga tgcccatggc ggccgcgatt tcggccacgg
    33301 cgagctcgcc gaaaaagcgg tagctgatcg cctcgcgctg tttgggcggc aattcgccga
    33361 gcgcctccgc gatcatgcgt ttccgctccg ccgtctcgat tgcgtcaggg ttgtccgggc
    33421 ggctgcgctg ctccgcggcc agacggtcgt ggatccgcgc gtgccgtttc cggtcgcgca
    33481 gagcattgag cgccgcgcgg taggcggccc gcatggcata ggccttcaga tcggccgggc
    33541 tgtccggccg ccggcggctc agcacccgca cgaggacatc ctgcgtgagg tccagagcct
    33601 cctcgcggtc ccgcaccatg cggcggatca ggccgaaagc gccgtcggag agccggcgca
    33661 tggccgcggc aacatcctcg ctcacatctg tctcctttcc ccgcggttac ctcgccacca
    33721 ccgacagcgc ataaagcgga aggtcggcgt cgtgcagttt cttcccttcg aagctccacc
    33781 ggacggtgcc cggttcctcc ggcgagagca ggccgttcga ctcgatgatt gtccccggca
    33841 ggttgagccg gatcgagaat tcgtcgctcg acagcacttc cgtgatccgc tggtcgcggt
    33901 gcacgcgttc gagcgcggcc tcaaactgct ccaggacggc cgcggccggc gtcccgacgg
    33961 cctcggccag ccgggcgcgg aacgtctccg cgaacgccgc ctccagactg tcgagggtga
    34021 gggtcaccgt ctcgtcgctc tgctgcatcg tccggaacag gaactcggtg gtgagcgtgc
    34081 gctcgaaatg ctcggccgcc cgggcggcaa tgaggtccat ggtcgccgcc tcgagcgacc
    34141 cgctccgcac gagttcggcc agcgcgttgc ggttcatccg ccagtgcttg aggctgaagg
    34201 ccgccgccaa ctgctcgaga tacttccgcc gctcgtcggg cgacaaattc accaggccgc
    34261 tgtcgatcgc cttggtctcc aggtcagcgt cccagagcca cggtgactcg gtgatgtcgt
    34321 attcaccgaa gcggcgcgcc tggtaggtgc gccggaactc gtagtacgtc cgatttccct
    34381 cggtccggac ggtgacggcg gtgggaaagt gcaggtcgag ctccggggag ggggtctcgg
    34441 ccgtgacgta cgattcgggc agcggcgccc cgtagggaac cgtccgcgag gctttcaggt
    34501 gcaactccgg ctgggcctgg gtggaatcga cgcgctgttc gataatagtc caggcgggcg
    34561 ccgacggcag ggcgaggggg gactgcaaga cctgggcgt
//
LOCUS       DAOPOE010000021        33417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1729_length_33417_cov_5.555213, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000021 DAOPOE010000000
VERSION     DAOPOE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33417
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_33417_cov_5.555213"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1076)
                     /locus_tag="PKM94_04655"
     CDS             complement(96..1076)
                     /locus_tag="PKM94_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD65963.1"
                     /translation="MHRASLFVWVAALAVCPLAAVSSGDVCGDVNGDGSVSVADVTGA
                     VNYLFRGIPEQPYFRPADVNGDYGVTVADLTWLVSYLFRGGPALECPATFTPAMGDGC
                     VEAWTSTAGGQPNPAVRSSGCLTVAPDDSAEYMFVELVGRDLHVHHVNAYYQCCLGYA
                     ATFEIDGRSVTVQEADTSFFPCPCMCYFNLEAILPGFAAAEPGEYAVTLVGLEGDTVG
                     ADTLVIGGPETMSLEVVGKDLRVRHLNAQANCCPAFLTDVFVNGGAITVIEWDTLSAC
                     DCLCTYNLLTVVNDLEPGDYAVTLIGVCTYPVLCAAIGTDTVTIPGRSAE"
     gene            complement(1214..2008)
                     /locus_tag="PKM94_04660"
     CDS             complement(1214..2008)
                     /locus_tag="PKM94_04660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOD65964.1"
                     /translation="MELSARDLSRFQRFAEDTARLAGELLKKRFHAAHRVRYKGRIDP
                     VTEADVKSERLITARLRRAFPGHALLAEEGSGTGAAAEFQWVVDPLDGTVNYAHGFPI
                     YCVSIALQFRGLPAAGAVYDPERDEMFRGARGQGAYCNRTRLRVAREIDLQRALLATG
                     FGYNVATARRNNLGYFARMVKRAQGVRRPGSAAIDLCWLAAGRIDGFWELDLHPWDTA
                     AAVLFIEEAGGKVTRTDGRPFAITDRSILAANPRLHASMLKVLSGR"
     gene            complement(2014..3828)
                     /locus_tag="PKM94_04665"
     CDS             complement(2014..3828)
                     /locus_tag="PKM94_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65965.1"
                     /translation="MSGARDYHEEEALGKAYDARLMRRLLTYMRPYRELTAGAILFIL
                     LAAAAQISLASITKWGIDSYIKAGRADGFHAVALVYLAAVCGILFFSWGQIMTTVRLG
                     QKVQHDLRMQVFEHLQRLHLRYFDRNPVGRLVTRVTNDVNTLNELFGTAVVAVIGDLF
                     MILLIVVYMLYLNWQLALITFLVVPVLVGATFVFRARAREAYRQVRLKLARINAFVQE
                     HVTGMAVVQLFTQEERAYREFGAINLDLQGAHLRSVIYYALFYPAVEIIGAVSLALVL
                     YFGGVRIIGGAMTFGELTAFIWLVERFYQPIRDLSEKYNVLQSSMASSERIFRLLDTA
                     PEISDPPRPRAIAAFRGRIEVENLWFAYEDEDWVLRDLTFSVEPGERVAVVGATGAGK
                     TSLVNVLFRFYDYQKGSIRLDGVEIRDLALRELRSHLGLVLQDVFLFSGDYAGNVRLR
                     NEGIGDEQVRQALSRVGFDRFLKHLPDGIRTEVKERGATLSTGQKQLLSFARALAFDP
                     GILILDEATSSVDTETEHLIQQALEELLAGRTSIVIAHRLSTIEKADKILVLHHGRLR
                     EMGRHEELLSRGGIYRTLYEMQYRRTAAAPVNRQAAAK"
     gene            complement(3825..5594)
                     /locus_tag="PKM94_04670"
     CDS             complement(3825..5594)
                     /locus_tag="PKM94_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD65966.1"
                     /translation="MNTTATQANTPPRQLATVTKYFRTYRWYFLAGLAVTVLSSGLMM
                     YTPRLVGQIIDGLEQGRGAGYAGRRVLILVGLTAASGVFMFLMRRTVIWASRYIETDL
                     RGEMFSHLLRLTPSYYHGTRTGDVMAAMTNDLEAIRMMAGPGVMYTADAAVSLLIGLV
                     MMLTISPSLTLWALLPLLVLPFGVHRIGSVIHKRNAKIQDHFGVLTAAAQENLAGMRV
                     VKAYGQEEREIENFRAVSETYIGLNISLAKVQGIFAPLMRLIAALSYLTVFYMGGLRV
                     INGTETLGTVITFFGYLALMTWPVIAIGWVVSLYQRGLASLARVNRLLHTEPEVPEGG
                     GRAAAGPMAGRIECRNLRFSYNGRPVLDGVNLMIEPGQTVGLLGLTGSGKTTLVNLIA
                     RLHPVERGMLFIDGVDVNDWPLRELRRQIGFATQEPFLFSDSIAANIRFGRPEAGLAA
                     VQQAARIAALDKDVAEFPQGYETMVGERGITLSGGQKQRTAIARAIVVDPRIIILDDA
                     TSAVDTETEHEISQRIKEVLTARTAIVISHRVSSVKDADQIVCFDDGRIIERGDHEAL
                     MARNGFYAQLYRSQLLEKELEAL"
     gene            complement(5657..7921)
                     /locus_tag="PKM94_04675"
     CDS             complement(5657..7921)
                     /locus_tag="PKM94_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65967.1"
                     /translation="MTTLRFMSGACLRRAFLSAAVSALAAIAVPPAARAADPAGYVLS
                     DVRVLYVSDDPERIDWPTLYYLNDEHGCRIDVVLLDERSSYRTAAAGLPERELYVHTL
                     LLPPGDREAMDSATAMLFSERYPDIVLFEALRGQGGYNALRERILAAEPSADRRFNVL
                     KIYEQVTDPNEALDSAAAVFLSRRELAVRYADRLAGEIPQLLGRDYRARAAAEPQLVR
                     YRQVVGRVGSRTAEVNFLAGVPGNRLLAVLEELVADGPKKTTLLRLGRQYLASLNAAL
                     SASGRERAAAIVEGYRALSDLVAAAAGDPLYDAQVDLRVYFAELLAQTEELALRSVGV
                     HWDGGITVRDSPDGPRLKFRASLSADGPQEVRFSNVRFHPYWDTAAVTLDTAARTVAP
                     HQTFAREYLVEIDPRYLEAGRPDSLVFTAEIAFGSNPLTVRSAIPVGERADLDVRFEP
                     DFCFVPPVADLDIDRVVAPLTLRVVIQKPREFAGTVKVNLETPRGLFAGAYRQEISLR
                     KGSLRETVRIPFSISKLFELGLQQQVISLAYRDNVVAADTALVRIASCHVDDKTQVAF
                     LPDTTGRLEDVLGMTDAAFRPLTDRGLATAPLEAYDVILVGSGAFRAYPSFAAMKDRF
                     EDFVRNGGSLVIFGQPVDWPQDVLPVRFVPDVELVDRNDIINRIPDANLLSKPYPIVE
                     TNLLSSFYRKQDVAAAVVAPAERVYVAPSGAALLSVSRIGEGQIIYCGLPLLDMIARL
                     DIDAIHLFANILNY"
     gene            complement(7990..8355)
                     /locus_tag="PKM94_04680"
     CDS             complement(7990..8355)
                     /locus_tag="PKM94_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD65968.1"
                     /translation="MRIHKLLDCPETTAGDGARLRELVHPDRPYPFEGRYSLAHGTVP
                     PGERTAAHVLTTDEVYHILGGKGILYVGEELALVEPGDTIEVPPATVQWIENPGAEPL
                     TFLCLVDPAWRPEDETIIR"
     gene            complement(8474..9259)
                     /locus_tag="PKM94_04685"
     CDS             complement(8474..9259)
                     /locus_tag="PKM94_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD65969.1"
                     /translation="MDIVCLIKQVPEIALIRVNESANKVELPQGPGTVNPFDEYAVEE
                     GLRLKEKHGGTVAVMTVGTERADAALRNCLALGVDEAYLLSDPAFEGSDPQAVARILA
                     AGLKKLGRFTLILAGKQAIDSDSAQVPGAVAALLDLPQAMFVKKVQSVDGGKIVVERT
                     TEDGYDVVELALPAVLSVVKEINEPRLPSLKGKMAAKKKEVAAWNAADLGIDPATVGA
                     HSATATMKVAPPPPRPKGERIDGETPEEIAERLFRKLRDNQIL"
     gene            complement(9397..9684)
                     /locus_tag="PKM94_04690"
     CDS             complement(9397..9684)
                     /locus_tag="PKM94_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65970.1"
                     /translation="MRKATKATRKKTAARRAVRPGAKAWTQGEVQELVRLYKETTAHE
                     VGRRLGRSLASVKAKIRTLKLRKSAAARRRTVAGRRGGAARTAGRKTARRR"
     gene            complement(9893..10645)
                     /gene="rnc"
                     /locus_tag="PKM94_04695"
     CDS             complement(9893..10645)
                     /gene="rnc"
                     /locus_tag="PKM94_04695"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOD65971.1"
                     /translation="MGVWDRLRRFFSPSQAQRRAAAPAAVQQLIGYHFRCPAVLELAL
                     THRSFAHAADQYHLSNERLEFLGDSVLGLVIAERLYQDNPDLREGELTKRKALLVSEA
                     TLAEIGVSTGLNRHLLLAPEEDRSGGRERASIVSDAVESIIGAVFLDGGFEAARDVVI
                     RLVYTREPVIATDASRQNFKGELLELVQSRGQGMPQYDVVAETGPDHEKVFRVEVTLN
                     GQTIGSGSGHSKKEAEQRAAEAALLHVRRAAD"
     gene            complement(10645..11895)
                     /gene="fabF"
                     /locus_tag="PKM94_04700"
     CDS             complement(10645..11895)
                     /gene="fabF"
                     /locus_tag="PKM94_04700"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOD65972.1"
                     /translation="MKKRVVVTGMGVVSPNGNTLEAFWQSLLAGKSGAGRITRFDPEP
                     FPTQIAAEVKDFNPELYLEKKEARRMDLSEQYAVAASDMALVDAGLKDGRLDPDRIGV
                     VIGSGIGGISTFERQHSLMEETGGPAKVSPFFIPMMIIDMCAGLVSIRYGFRGPNYAT
                     VSACASSAHAIADAFRIIQRDEADVMVTGGAEATITRTALAGFCQAKALSVRNDEPER
                     ASRPFDAERDGFVMGEGAAILVLESLDHARARGVRIYAELLGAGMSGDAHHMTAPHPE
                     GLGARRAMAMALRDAGLAPEQIDYVNTHGTATILGDTAETRAIKEVLGPRAYEIPCNS
                     TKSMTGHLLGAAGALEVAACVLSLRDSVVHPTINIDTPDPECDLDYVREGKRAVTIRH
                     ALSNSFGFGGHNVSLVVGTVNGSA"
     gene            complement(11937..12185)
                     /locus_tag="PKM94_04705"
     CDS             complement(11937..12185)
                     /locus_tag="PKM94_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD65973.1"
                     /translation="MAAELEDRVKEIIVEQLGVEASQVTEKAKFVDDLGADSLDTIEL
                     VMALEEEFSIEIPDEDAEKIVSVGDAITYIAEHSPKAK"
     gene            complement(12230..12961)
                     /gene="fabG"
                     /locus_tag="PKM94_04710"
     CDS             complement(12230..12961)
                     /gene="fabG"
                     /locus_tag="PKM94_04710"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOD65974.1"
                     /translation="MDFSGKTVVVTGSARGIGRAIAERFARAGARVVISDVDDAALAA
                     AAAAMPGEAIGVRADVTSSADAEALIAAAQERFGSVDVVVNNAGVTRDTLLVRMDEKD
                     WDMVLDINLKGAFLVTKSAAKVMMKQRSGRIINVSSVVGLFGNAGQTNYAASKAGLLG
                     LTKSAAKELGARGITVNAVAPGFIETDITRVLPEEARARFLERSVIKRAGTPDDVAAA
                     VMFLASDAAAYITGHVIAVDGGMAM"
     gene            complement(12977..13912)
                     /gene="fabD"
                     /locus_tag="PKM94_04715"
     CDS             complement(12977..13912)
                     /gene="fabD"
                     /locus_tag="PKM94_04715"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOD65975.1"
                     /translation="MSKTALFFPGQASQYVGMGRDLYEASADVRRLYELAADAMQFDI
                     ARLSFEGPAEDLRRTRFTQPAILLHSLAALTVLGDGRPAFDYAAGHSLGEYGALAVTG
                     ALTFEQAIRAVVRRAHLMEEACRQQPGTMAAVMGLSAAQVEQVCREAAAAGVVVPANY
                     NSGIQIAVSGSRPGVEKAIELAKSAGAKRAMLLEVGGAFHSPLMEPAREGLRTYLADL
                     AIAAPARAVIANVTAEPATDGATIRRLLIEQVTAPVKWAQTMACLAAEGVTTVYEIGP
                     GKVLTGLARRDMNLTTSVNLDTLADIEALGALQRP"
     gene            complement(13915..14901)
                     /locus_tag="PKM94_04720"
     CDS             complement(13915..14901)
                     /locus_tag="PKM94_04720"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOD65976.1"
                     /translation="MGQIKARIIGTGSYVPPRRLTNAEFEKTIDTSDEWIVTRTGIRE
                     RRIAANGTATSEMATEAARAALAMSDLAPDQIDLVIIGTVTPDYRVPATACLVQERLG
                     LVNAVAFDIAAACTGFISGLTIASSFISSGQHTNALVVGAEKLSSITNWNDRGTCILF
                     GDAAGAAVVTGDDSGRGLLASFMRSDGRYSQLLWQKVGGTAFPYTPEFPFDGSDKLVM
                     NGSEVFKVAVREMANAAERVVADAGIDSNDIALIVPHQANIRIIEALAKRMKVSMDRV
                     YLNIDRYGNTSAASVPLALDEANRAGMLHPGDHILLVAFGGGLIWGSALVRW"
     gene            complement(14831..15928)
                     /gene="plsX"
                     /locus_tag="PKM94_04725"
     CDS             complement(14831..15928)
                     /gene="plsX"
                     /locus_tag="PKM94_04725"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOD65977.1"
                     /translation="MATDSAHTILLDVMGADHGPEGIIAGGIEAARLIGPAARVVLVG
                     HRPTIDRVLAGTVNVPPAVGVLHAETEVPMTIAATDGARMRDSSVAVGLREVRERRAD
                     AFVSPGNTGAVMATALLTLGRIEGVMRPAITTMFPTSTGRPLVLLDCGANVDCKPTHL
                     SQFAVMGSIYSSIMFNLEAPRVGLISIGEERSKGNDLVFSARKLLAASHINFVGNIEG
                     RDILSGTVDVGVTDGFTGNILLKFAESVQPMLAKAVQRQIQTNIFSRIGAFLLLPFLR
                     RMAKQFDYAQSGGAPLLGVDGTVIICHGSSSSLAIQNAVLAAYEMAAKGIRERIHEEL
                     LTNQLGNTNGANKSKDNRDWVVCTASAPDER"
     gene            complement(15980..16162)
                     /gene="rpmF"
                     /locus_tag="PKM94_04730"
     CDS             complement(15980..16162)
                     /gene="rpmF"
                     /locus_tag="PKM94_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOD65978.1"
                     /translation="MPLPKRRHSRTRGRKRRTHWKLSAPNVSECPHCHQPRLPHHVCT
                     HCGYYNGRPIIQPKEA"
     gene            complement(16176..16700)
                     /locus_tag="PKM94_04735"
     CDS             complement(16176..16700)
                     /locus_tag="PKM94_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOD65979.1"
                     /translation="MKLVLRDFETFPAHVCLEAEPGTLPVDYPGVVEVGRVTVDLDIQ
                     KSGEEFFCQGQVAAEVGVECSRCLVRFEEQLHGETDFIVCSYDTWVEAHAEAEDDEEY
                     VFYEGRSLEVDLGEPVRQAVIVALPMTPLCREDCKGLCPKCKINRNEQTCDCAFDDVD
                     PRWNKLRDLRGESD"
     gene            complement(16825..17232)
                     /gene="atpC"
                     /locus_tag="PKM94_04740"
     CDS             complement(16825..17232)
                     /gene="atpC"
                     /locus_tag="PKM94_04740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOD65980.1"
                     /translation="MFTLSIVTPEKVFFEAEIRSLVVPGSEGYLGILSDHAPLITALK
                     PGKIEFRDAADKVRVMAVSGGFLEVSHNTATLLADAVEWAEEIDIARARAALERNQQA
                     LAAAQAKPGTDLGGLPDAIERARNRIRVYEARR"
     gene            complement(17246..18652)
                     /gene="atpD"
                     /locus_tag="PKM94_04745"
     CDS             complement(17246..18652)
                     /gene="atpD"
                     /locus_tag="PKM94_04745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044045207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD65981.1"
                     /translation="MAENIGRVVQVIGPTVDCEFRSDALPNILNAIRIVDQARNIDLT
                     VEASMHVGDKIVRCVALASTDGLVRGMPAIDSGSPITVPVGDQVLGRVFDLLGRPIDS
                     KGEVKEPRKRLPIHRPAPPFEEQATKAEMFETGIKVIDLLEPYVKGGKVGMFGGAGVG
                     KTVIIQELIHNIATEHGGYSVFCGVGERTREGNDLWLEMTESGVINRTAMVFGQMNEP
                     PGARLRIGLSGLTMAEYFRDEEGQDVLIFIDNIFRFVQAGSEVSALLGRMPSAVGYQP
                     TLGTEMGGLQERITSTKAGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIAE
                     LGLYPAVDPLDSTSRILDPQIVGEEHYAVARAVQKILQRYKDLQDIIAILGIDELSEE
                     DKITVSRARKIQRFLSQPFFVAEAFTGRKGVYVKIEDTIRGFRMIIDGELDHVGEQQF
                     YMAGGIDEVLDRYEKSRK"
     gene            complement(18709..19575)
                     /gene="atpG"
                     /locus_tag="PKM94_04750"
     CDS             complement(18709..19575)
                     /gene="atpG"
                     /locus_tag="PKM94_04750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOD65982.1"
                     /translation="MPTLRDVKKRIRSVRSTRRITGAMEMVAAAKLRRAQQKVEQTRP
                     YADKLEEMLAHLAAGSSNEIVHPYFEERPVRKRTLVLFTGDRGFCGSYNANIIRDTLA
                     WLRDHTAVATELVVIGKKGYDYFRRRPQAVVGAHPGFNTTLDYGKARDIVGFLTHRFV
                     SGETDRIDLLYTRFLSLSKYRITLQSYLPIRPPAVGAAAPGGADYIFEPDAEHIYAAL
                     MPSYATTMFVTALVESFASELGSRMIAMNAATKNAGEMIDRLTLDYNKARQAQITKEL
                     LEVVSGAEALKG"
     gene            complement(19582..21144)
                     /gene="atpA"
                     /locus_tag="PKM94_04755"
     CDS             complement(19582..21144)
                     /gene="atpA"
                     /locus_tag="PKM94_04755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD65983.1"
                     /translation="MGLNPEEVTSVIKKELEKYETRLEMESVGTVLQVGDGIARIWGL
                     EDVQMSELIEFPGDIMGLVLNLEEDNVGAAIFGSDTHIHEGDTVRRTGRVASVPVGEA
                     LLGRVVNPLGQPLDGKGPIVTDKFRVIEGHAPGVVDRQPVKEPLQTGLKAIDSMIPIG
                     RGQRELVIGDRQTGKTALVIDTIINQRHSDVYCIYVAIGQKASTVAQVVETLRRYGAM
                     EYTTVVAATATDPAPLQYIAPYAGCAMGEEFMYNGKHALVVYDDLSKQAQSYRQLSLL
                     LRRPPGREAYPGDIFYTHSRLLERAAKLNEKHGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLEGELFFAGVRPAINVGISVSRVGGNAQTKMMKQVAGSLKLDLAQFREL
                     EAFTQFGSDLDEATLRQLNRGRKMVELLKQGQYVPMSVEHQVMIIWVGTKGYLDDVPA
                     EAVKTFEAEFLAFCEKEFPDIAHNLSKEKKISESTEKELQAAVAKFKAQFAASAPGAA
                     PAAAGPGPHKQV"
     gene            complement(21241..21789)
                     /gene="atpH"
                     /locus_tag="PKM94_04760"
     CDS             complement(21241..21789)
                     /gene="atpH"
                     /locus_tag="PKM94_04760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOD65984.1"
                     /translation="MLAQEVAQRYAHALFDAAREKGLLDAGYSQMADLRTVVRADRTL
                     LNFLTAPQVLDEHKIELLRTVFGSRLQRLFVEFLVVLVGKHRINYLVEIIDEFVERVE
                     KAKGIGRARVISAVPLSGPERENLVGRLSAKTGLQIRLEVRIDPAILGGLIVILHNEI
                     IDGSIRHGLGLIEEQLLKVKVN"
     gene            complement(21797..22279)
                     /gene="atpF"
                     /locus_tag="PKM94_04765"
     CDS             complement(21797..22279)
                     /gene="atpF"
                     /locus_tag="PKM94_04765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOD65985.1"
                     /translation="MNFELQQILTHALGFLLTVWILKRYAWGPLMNIMEERRSRIAGE
                     FETIEKEKGKVAAMAAEYEAKLKQIESERRQKLVEAVTEGKRIAEDLKAQARDEAREI
                     AQKARTDLEREVAKARVELKEQMVAITMGAAEKLLREKLDDARHRQLIGRFIDSVEKA
                     "
     gene            complement(22334..22570)
                     /gene="atpE"
                     /locus_tag="PKM94_04770"
     CDS             complement(22334..22570)
                     /gene="atpE"
                     /locus_tag="PKM94_04770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOD65986.1"
                     /translation="MDYQAMLGLAAPLAVGLAAIGSGLGIGRAVGSAMEAIGRQPEAA
                     GKIQTAMIIGAAFIEALTIYVFIVYILASGKIGG"
     gene            complement(22631..23527)
                     /gene="atpB"
                     /locus_tag="PKM94_04775"
     CDS             complement(22631..23527)
                     /gene="atpB"
                     /locus_tag="PKM94_04775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOD65987.1"
                     /translation="MLAYYLTVVATTAAKYAPALAAGVSAEAGGHGAAVSDGHAAGGA
                     HAGPPHLPSLIWLIGWAFGIEDVAWVRFLEHWINVFFAFVVAIFFTIVAMSVYRRRQM
                     IPGRLQNFLEWCVESMYNFIYSILGHETRKYLPFLGTLFFYILALNWIGMIPLGHSPS
                     TSINITASMAVLVFLYAQWTGIRRLGIGGYLYHLAGSPVGAVGWSMVIIMFPLHIIGE
                     LAKPFSLSLRLFGNITGEDTLIAVFVTLGIMLISFSPVGVPLQIPFYFLGLLLSTIQA
                     LVFTLLSTIYILLMLPHDEAAH"
     gene            complement(23528..23944)
                     /locus_tag="PKM94_04780"
     CDS             complement(23528..23944)
                     /locus_tag="PKM94_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65988.1"
                     /translation="MNETIDLGFINRTLKTTGLVLLIFVPFGIYYAGIYPTVAVLSGG
                     VWGMVNLMLLTRLVRAAIRPGGANKMEVAGVALVKFPLLYASGYFLATTPLFGPLALL
                     AGFSVPLAVMVLKVAARALLGADRSAGKRGQLQRAV"
     gene            complement(23937..24197)
                     /locus_tag="PKM94_04785"
     CDS             complement(23937..24197)
                     /locus_tag="PKM94_04785"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD65989.1"
                     /translation="MGLLSSGPDDQSGRDLRQIGLLTAVPFLLLGGVLVGYLIGNWAD
                     RKLGTEPYLAALGVLMGVAAAALEIVQVVRKASADDSEKRHE"
     gene            complement(24468..24881)
                     /locus_tag="PKM94_04790"
     CDS             complement(24468..24881)
                     /locus_tag="PKM94_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HOD65990.1"
                     /translation="MSAADLPSLNAVLNGTSTLLLLAGYAAVKRGRADIHRRFMLAAL
                     ATSALFLASYLVYHARVGSVPYPRHDWTRPVYFGILIPHVILAGVMVPFILLAVRHAW
                     RRRFDRHVRITRWVWPVWLFVSVSGVAIYLMLYGI"
     gene            complement(24878..25465)
                     /locus_tag="PKM94_04795"
     CDS             complement(24878..25465)
                     /locus_tag="PKM94_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOD65991.1"
                     /translation="MSKIITIGVILAAVLGGVALVTLYQADKARSELPVLGTVGPFTL
                     TDQFGEPFTDADLTDKLSVVEFFFTGCRGPCPIMNGHFADLYRAFAETDRVQFVSITV
                     DPEADSTARLADYAARFGVSDRRWVFLTGPLDSIVRISETYFMLAADDLPGNHSTRFA
                     LVDGRHRIRAYYSGTDAASVDVLRTHLRELLDDLP"
     gene            complement(25462..26409)
                     /locus_tag="PKM94_04800"
     CDS             complement(25462..26409)
                     /locus_tag="PKM94_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOD65992.1"
                     /translation="MKSLWRMALVSTIATYVLIFIGGLVRVSGAGLGCPDWPKCFGRW
                     MPPTDVSQLPPDIDPSRFNFTLAWIEYTNRLSGVIVGLLIAATAVLALVRARRTPGIL
                     WPAVAAGLLTALQGWQGRAVITSELAPLVVTVHTVLALVIVSLLIWAAAGAYRQVHPR
                     EAGEGLAAWAQRRLLLLWGLAIVQVMLGTRVREGLEAMREDHPAWSAAKAIMAIGAVS
                     HLHMALGIVVAAVTWWTGRGLLRAPGGSAPAVRQAAVAAMVLVGLQILSGLTFIFWDI
                     PPVVQVFHLWIASLYIGTLLLALAAARQPGPVPAGEMRG"
     gene            complement(26523..26915)
                     /locus_tag="PKM94_04805"
     CDS             complement(26523..26915)
                     /locus_tag="PKM94_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOD65993.1"
                     /translation="MASATHVHTEHVLPLRVYFGVFAALLVLTALTVFIARFHFGALN
                     LVVAMAVAAVKAGLVALFFMHLKYDSKVYAAVFITAVVFLAVFIVLTMSDTMTRGHVD
                     PEKQSPIIPEAVIYRQAAPSADTAAAGG"
     gene            complement(26922..27596)
                     /locus_tag="PKM94_04810"
     CDS             complement(26922..27596)
                     /locus_tag="PKM94_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HOD65994.1"
                     /translation="MAKERMEAPGAAGHGHPKHLAHHFSDPAQQKDSAKLGMWIFLLT
                     EVLLFGGLFCAYAVYRAWNPEIFHNGHAFLNTTRGTVNTLVLITSSLTVALAIRFLQM
                     DNRRAAAANLAATLALAGTFLVIKYFEYAHKFHEGQLPGKFYTFQGMEGTNPHVFFSV
                     YFLMTGLHGIHVLVGMGVITWVLARTLRNHFSAEYYTPVELTGLYWHLVDLIWIFLFP
                     LFYLVG"
     gene            complement(27599..29242)
                     /gene="ctaD"
                     /locus_tag="PKM94_04815"
     CDS             complement(27599..29242)
                     /gene="ctaD"
                     /locus_tag="PKM94_04815"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOD65995.1"
                     /translation="MAEATAVNYLNATRGWKSWLLTLDHKRIAILYMAAIAVFFFVGG
                     VLAMLIRLELLHPGRDLVGADTYNKLFTLHGAIMIFLFIIPGVPAILGNFLLPLMLGA
                     RDVAFPRLNLASWYIFMLGSLFALYSIVAGAADTGWTFYTPYSTQTDTSVISMTLGAF
                     ILGFSSIFTGLNFIVTIHKLRLPGMTWYRMPLFVWSMYATAIIQVLATPVLGITLLLL
                     VIERVAGIGIFDPAMGGDPVLYQHFFWFYSHPAVYIMILPGMGIVSELITAHAHKKIF
                     GYKAVAYSSLGIAFVSFLVWGHHMFVSGQSELAAMLFSFLTFFVGIPSGIKVFNWVTT
                     LYRGSIKLTTPMLYALSFLFLFSIGGFTGIMLGALAVDVHVHDTYFIVAHFHYVMMGG
                     TVIAFLGGLHHWWPKMFGRMYNEPWAKVACALIFVGFNVTFGSQFLLGLHGMPRRYYN
                     YLDQYQPLHAFSTFGSWILGAGFVIMAIYLIHSLARGRRAGSNPWRALTLEWETPSPP
                     PTENFPFTPSLAHGPYDYDRVIPVNVEPGGRAVRPEVVS"
     gene            complement(29247..30215)
                     /gene="coxB"
                     /locus_tag="PKM94_04820"
     CDS             complement(29247..30215)
                     /gene="coxB"
                     /locus_tag="PKM94_04820"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOD65996.1"
                     /translation="MDTTGTLFFPPAGSTLAGEVDSLFYFIFWASAVLFAIVIGLTVF
                     FIIRYRRRRPTGTTYGRDHNLALEITWTAVPTVLILIVFVWGFRAYMRMTIVPRDALE
                     VKVTAQKWFWTFDYPNGANSVNELVAPVDRPVKLLMSSRDVIHSLFVPDFRMKMDVLP
                     NRYSVTWFEATRPGTFNLFCTEYCGTGHSEMLAKVKVVSEREFAEFLASTGGPGAGES
                     PADYGRRLYTSRACNTCHTLDGKPSVGPTWQGLWGRREALQTGDFVTVDENYIRESVL
                     EPQARVTAGFQPVMPTYQGILTDRDIDALIAFIKSLDTTNSEAAGE"
     gene            complement(30225..31055)
                     /locus_tag="PKM94_04825"
     CDS             complement(30225..31055)
                     /locus_tag="PKM94_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOD65997.1"
                     /translation="MQRVLRNIGFLALLAAAGGAAGQTVRTDPPELRGIDVHERLGDT
                     IPRGLGLVDEAGREVALGDYFGGDRPVLLVLGYYECPMLCNLVFNGLTEALPRVGLDL
                     GRQYRVVTVSIDPGETPDLAAAKKASYLAQLGPAAPPASWAFLTGDSAAIAQLADAVG
                     FRYYYDESRDEYAHPAVVHVLSPEGVVSRYLHGIQYNPRDLKLALVEAADGKIGSTLD
                     RVILYCYHYDPGAGGYVVLAQNVMLVGGLLTVLGLGGFLLVLWLRESYRTRHRPAPAG
                     "
     gene            complement(31172..31519)
                     /locus_tag="PKM94_04830"
     CDS             complement(31172..31519)
                     /locus_tag="PKM94_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65998.1"
                     /translation="MTDTENKPAPGREEKAGYDTSDVNVTKALVVGVLLTLLVIAFAV
                     VLNEYFIAVTEEISYEQQRAPESKALRELRAREVEQLESYGVQDGAAGSYRIPIERAM
                     ELQAQEAFTAKAR"
     gene            complement(31529..32710)
                     /locus_tag="PKM94_04835"
     CDS             complement(31529..32710)
                     /locus_tag="PKM94_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD65999.1"
                     /translation="MTIDPQTYRVSGGSRRAAAPLAIGVAGLALSAVACAVDPHRFFP
                     SYLTAFTYWVTIGLGGLFFTMLHHLVNAKWSVVLRRAAENIMSGLPLLAVFAVPLLFG
                     MHDLYHWTHAEAVAEDPLLQAKAAYLNVPFFIVRMVFYFAVWGLLAYRLNRISDLQDR
                     GGPEDVRRRLRVVSAPGMLLFALTSTFAAFDWLMSLDPHWYSTIFGVYIYSGGFLGAL
                     GLLVVLMSYQRRRGLLREVVTVEHYHDLGKLMFAFVIFWAYMAFSQYFLIWYTNVPEE
                     TVWFLVRWQEPWKAVSLAIVFGHFGLPFVALVFRATKRNVAALTTVSLWLLAIHWVDL
                     HWIVQPNFPGAGAVFSWIDAATMAGIGGIFIWYVWRRHLRRPLVPVGDPGLQRSMQLT
                     N"
     gene            complement(32721..33347)
                     /locus_tag="PKM94_04840"
     CDS             complement(32721..33347)
                     /locus_tag="PKM94_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOD66000.1"
                     /translation="MRARLTYGRAAMLVLLAASLLLAGCYRGRPSQRTPIHMVRDMDS
                     QPKYQPMERTEFFGNEAIMRTPPAGTVARGQLRGDAVYFTGVTPAGDTVRQLPVAVTL
                     PFLQRGRERFDIYCSPCHGRVGDGKGIMVQRGYVPPPTFHDARLRSVADGHIFDVITH
                     GLRNMPAYQHQISVEDRWAIVAYLRALQRSQNASAADVPEEIRRGLRP"
BASE COUNT         5768 a        11320 c        10798 g         5531 t
ORIGIN      
        1 gcattgattc acctccttcc ttggactgtt gtgcgcctcc aagaaagaag gagtctgtgg
       61 gtccgtcaag cacttttttg cgcgccggcc gcggatcact cggccgaccg gccgggtatg
      121 gtcacggtgt cggttccgat cgcagcgcac agcaccggat aggtgcacac cccgatgagc
      181 gtgaccgcgt agtcgcccgg ctcgagatcg ttgaccacgg tgagcagatt gtacgtgcac
      241 aggcagtcgc aggcggagag cgtgtcccac tcaatcacgg tgatcgcgcc cccgttgacg
      301 aagacgtcgg tgaggaaagc ggggcagcag ttggcctgcg cgttcaagtg ccggacgcgc
      361 agatccttgc cgaccacctc cagcgacatc gtctccggcc cgccgatcac gagcgtgtcc
      421 gcgccgaccg tatccccttc caggccgacc agcgtgaccg catactcgcc cggctcggcg
      481 gccgcgaagc cggggaggat cgcctcgagg ttgaagtagc acatgcacgg gcaggggaag
      541 aaagaggtgt cggcctcctg gaccgtgacc gagcggccgt cgatctcaaa cgtcgccgca
      601 taccccagac agcactggta gtatgcattg acatggtgga cgtggagatc cctcccgacc
      661 agctcgacaa acatgtactc cgcggagtcg tcgggagcca cggtcagaca ccccgaggaa
      721 cggaccgcgg gattcggctg gccgccggcc gtcgacgtcc aggcttcgac gcagccgtcg
      781 cccatcgccg gggtgaacgt ggccgggcac tcgagggcgg ggccgccccg gaacaggtag
      841 gacaccaacc aggtgaggtc ggccacggtg acgccgtagt ccccgttgac gtcggccgga
      901 cggaaatacg gctgctcggg aattccccgg aagaggtagt tgacggcacc ggtgacgtcc
      961 gcgacgctca cggagccgtc gccgttcacg tccccgcaca cgtcccctga tgataccgcc
     1021 gcgagcgggc agacggccag cgccgcgacc cagacgaaaa gtgatgcgcg atgcatatca
     1081 acctcgctca agtaggggtc gaaagagcgt tccgaatggt cgaaggagtc cgggcatgaa
     1141 gcgagaataa tatacgcgcc gccccacatt tgagcaaaca ctcatctgcc gcggcgtggg
     1201 cgggacacga taactatctg cccgacagga ccttgagcat cgatgcgtgg agccgcgggt
     1261 tggccgcgag gatggaccgg tcggtgattg cgaacggccg gccgtcggtc cgggtcacct
     1321 tcccccccgc ctcttcgatg aagagcacgg cggcggcggt gtcccacggg tgcagatcga
     1381 gttcccagaa tccgtcgatc cgtccggccg ccagccagca gaggtcgatc gccgccgatc
     1441 cgggccgccg cactccctgg gcgcgcttca ccatgcgcgc gaagtagccg aggttgttgc
     1501 gccgggcggt ggccacgttg tagccgaacc cggtcgccaa aagggcgcgc tgcaggtcga
     1561 tctcgcgggc cacccgaagg cgggtgcggt tgcagtacgc cccctgcccg cgcgcccccc
     1621 ggaacatctc gtcccgttcg ggatcgtaca ccgcgccggc ggccgggagc ccccggaact
     1681 gcagcgcgat cgagacgcag tagatcggga aaccgtgggc gtagttgacg gtgccgtcga
     1741 gcggatcgac cacccactgg aactcggcgg cggcgcctgt cccggatccc tcttcggcca
     1801 ggagcgcgtg tcccgggaac gcgcggcgga ggcgcgcggt gatgagacgt tcggatttga
     1861 catccgcctc ggtgaccggg tcgatgcggc ccttgtagcg gacgcggtgg gcggcgtgga
     1921 agcgcttctt gagcagctcc ccggcgaggc gcgcggtgtc ttcggcaaag cgctgaaaac
     1981 ggctcaggtc gcgggccgac agttccatgg tcctcacttg gcggccgcct gccggttgac
     2041 cggcgccgcg gcggttctcc ggtactgcat ctcgtacaag gtacgataga tccccccccg
     2101 gctcaagagc tcttcatgcc gccccatctc gcgcaggcgg ccgtggtgga gcaccagaat
     2161 cttgtcggcc ttctcgatag tcgagagccg gtgggcgatg acaatcgagg tccgcccggc
     2221 gagcagctcc tcgagcgcct gctggatgag gtgctccgtc tcggtgtcca ccgaactggt
     2281 ggcctcgtcc aggatgagga tccccggatc gaacgccagc gcccgggcga acgacagcag
     2341 ctgcttctgc ccggtcgaaa gcgtcgcccc ccgctccttc acctcggtgc ggattccgtc
     2401 gggaaggtgc ttcaaaaagc ggtcgaatcc gacgcgcgag agcgcctggc ggacctgttc
     2461 gtcgccgatg ccctcgttgc gcagccggac gttgccggca tagtcgccgg agaagaggaa
     2521 cacgtcctgc agcaccagcc cgaggtgcga gcgcagctct ctgagggcga gatcgcggat
     2581 ctcgacgccg tcgagccgaa tcgatccctt ctgatagtcg tagaagcgga agagcacgtt
     2641 cacgagcgaa gttttgccgg ccccggtggc cccgaccacc gccacccgct cgcccggctc
     2701 gaccgagaac gtgaggtcgc gaagcaccca gtcttcgtcc tcgtaagcga accagaggtt
     2761 ctcgacctcg atgcgcccgc ggaaggcggc gatcgcccgc ggccgcggcg ggtcggaaat
     2821 ctccggcgcc gtgtccagga gtctgaaaat gcgctccgac gacgccatcg acgactgcag
     2881 cacgttgtac ttttccgaga gatcgcggat cggctggtag aaccgctcga ccagccagat
     2941 gaaggcggtg agttcgccga acgtcatggc gccgccgatg atccgcacgc ccccgaagta
     3001 gaggacgagc gcgagcgaca cggcgccgat gatctcgacc gccgggtaga acagcgcgta
     3061 gtagatcacc gagcgcaggt gcgcaccctg gaggtcgagg ttgatggcgc cgaactcgcg
     3121 gtaggcgcgt tcttcctgcg tgaacaactg cacgaccgcc atgccggtca cgtgctcctg
     3181 gacgaaggcg ttgatgcgcg cgagtttcag gcgcacctgg cggtaggcct cgcgcgcccg
     3241 cgcccggaac acaaaggtcg cccccaccag caccggcacc acgaggaagg tgatcagcgc
     3301 gagctgccag ttgaggtaga gcatgtagac gacgatgagg aggatcataa agaggtcgcc
     3361 gatcaccgcc accaccgccg tgccgaacag ctcgttgagc gtgttgacgt cgttggtgac
     3421 ccgcgtcacc agccgcccca ccgggttgcg gtcgaaatag cgcaggtgca ggcgctggag
     3481 gtgctcgaac acctgcatcc gcaggtcgtg ctgcaccttc tgccccagcc gcacggtcgt
     3541 catgatctgc ccccaggaga aaaacaaaat cccgcagacg gcggcgagat agaccagcgc
     3601 gaccgcgtgg aacccatccg cccggccggc cttgatgtag ctgtcgatcc cccacttggt
     3661 gatggaggcc agcgaaatct gcgcggccgc ggccaggagg atgaacagga tggcgccggc
     3721 cgtgagctcg cggtagggcc gcatgtaggt caggaggcgg cgcatgaggc gcgcgtcgta
     3781 cgccttcccg agcgcctctt cctcgtggta gtcccgggcg ccgctcacag cgcctccagt
     3841 tctttttcca gcagctgcga cctgtagagc tgggcataga agccgttgcg cgccatgagc
     3901 gcctcgtggt cgccgcgctc aatgatccgg ccgtcgtcga aacacacaat ttggtccgca
     3961 tccttgaccg acgacacccg gtgcgagatc acgatcgccg tccgcgccgt cagtacctcc
     4021 ttgatgcgct gactgatctc gtgctcggtc tcggtgtcga ccgcgctggt cgcgtcgtcg
     4081 aggatgatga tccggggatc gaccacgatc gcgcgggcga tggcggtgcg ctgcttctgg
     4141 cccccggaga gcgtgatgcc gcgctcgccc accatcgtct cgtacccctg ggggaattcg
     4201 gcgacatcct tgtcgagggc ggcgatccgg gcggcctgct gcaccgcggc caggccggcc
     4261 tcgggacggc cgaaccggat gttcgccgcg atggagtcgg aaaacaggaa cggctcctgg
     4321 gtggcgaaac cgatttgccg ccgcagctcg cgcagcggcc agtcgttcac gtcgacgccg
     4381 tcgatgaaga gcatgccacg ctcgaccggg tggaggcggg cgatgagatt caccaacgtc
     4441 gtcttgcccg aaccggtgag gccgaggagc ccgactgtct gccccggttc aatcatcaga
     4501 ttgaccccgt cgagcaccgg ccggccgttg tagctgaaac gcaggttgcg gcactcgatg
     4561 cgccccgcca tcggcccggc cgcggcccga cccccgccct ccgggacctc gggctccgtg
     4621 tggagcagcc ggttgacccg ggccagcgag gccaaccccc gctggtagag cgagacgacc
     4681 cagccgatgg cgatcacggg ccaggtcata agcgccaggt agccgaaaaa ggtgatgacg
     4741 gtgccgagcg tttcggtccc gttgatcact ctcaggcccc ccatgtagaa aaccgtgagg
     4801 taggagaggg cggcgatgag gcgcatgagg ggggcgaaaa tcccctgcac tttggcgagg
     4861 ctgatgttca ggccgatgta ggtttcggag acggcgcgga agttctcgat ctcgcgctcc
     4921 tcctggccgt acgccttgac tacccgcatg ccggcgagat tctcctgcgc cgccgccgtg
     4981 agcacgccga agtggtcctg gattttcgcg ttgcgcttgt ggatgaccga gccgatccgg
     5041 tgcacgccga agggcagcac gagcagcggc agcagcgccc acagggtgag cgagggggag
     5101 atggtgagca tcatgaccag cccgatgagc aggctcacgg ccgcgtcggc cgtgtacatc
     5161 acccccggac cggccatcat ccggatcgcc tccaggtcgt tcgtcatcgc cgccattaca
     5221 tccccggtcc gcgtgccgtg atagtacgag ggggtcaggc ggagtaggtg ggagaacatc
     5281 tcgccgcgca ggtcggtctc gatgtagcgc gaggcccaga tgacagtgcg gcgcatgagg
     5341 aacatgaaga cccccgacgc ggccgtcagc ccgaccaaaa tgagcacccg ccgcccggcg
     5401 tagcccgcgc cccggccctg ctcgagcccg tcgatgatct gccccaccag ccgcggcgtg
     5461 tacatcatca ggccggagga caatacggtc accgctaaac cggcgaggaa ataccagcgg
     5521 tacgtgcgga agtatttcgt gacggtggcg agctgccggg gcggggtgtt cgcttgtgtt
     5581 gccgtcgtgt tcattcccta ccaatgccgg aactgccgac cgtcggcagc cgaacctctt
     5641 ataacaccgg ccggagctaa tagttcagaa tgttcgcaaa caggtgaatg gcgtcgatat
     5701 ccagccgggc aatcatgtcc aggaggggca ggccgcagta gatgatctgt ccctcgccga
     5761 tccgcgagac cgacaaaagc gcggcgccgc tcggggcgac atagacccgc tcggccggcg
     5821 ccaccaccgc cgccgccacg tcctgcttcc ggtaaaacga cgagagcagg ttggtctcga
     5881 caatcgggta cggtttggag agtaagttgg cgtcggggat gcggttgatg atgtcgttgc
     5941 ggtcgaccag ctccacgtcg ggcacgaacc gcaccgggag aacatcctga ggccagtcaa
     6001 cgggctggcc gaaaatcacc agcgacccgc cgttgcggac gaaatcctcg aatcgatcct
     6061 tcatggccgc gaacgacgga taggcgcgaa acgcacccga accgaccagg atcacgtcgt
     6121 acgcctccag cggagccgtc gccagccctc ggtcggtgag cgggcggaaa gccgcgtccg
     6181 tcattcccag cacgtcttcg agccgcccgg tcgtatcggg caggaacgcc acctgggtct
     6241 tgtcgtcgac gtggcaggag gcgatccgca cgagcgcggt gtcggccgcc accacgttgt
     6301 cgcgataggc caaagatatc acctgctgct ggaggccgag ttcaaacagc ttcgaaatcg
     6361 aaaacggaat gcgcaccgtc tcccgcagcg atcctttccg gagcgagatt tcctgccgat
     6421 aggccccggc gaacagcccg cgcggcgttt ccagattgac cttgactgtc cccgcaaact
     6481 cgcgcggttt ctgaatgacc acccgcagag tcagcggcgc caccacccgg tcgatgtcga
     6541 ggtccgcgac cggcggcacg aagcagaagt cgggctcaaa ccgcacatcg aggtccgccc
     6601 gctccccgac cgggatcgcg ctccgcacgg tcagggggtt cgagccgaac gcgatctcgg
     6661 ccgtgaacac gagcgaatcg ggacggccag cctcgaggta gcgggggtcg atttccacca
     6721 ggtactcccg tgcgaacgtc tggtgcggag ctaccgtgcg ggcggccgtg tcgagcgtca
     6781 ccgccgccgt gtcccaatag gggtggaacc ggacgttgct gaaacggact tcctgcggcc
     6841 cgtcggccga caggctcgcc cgaaatttca gccgcggacc gtcgggggag tcgcggaccg
     6901 tgatgccgcc gtcccagtgc accccgaccg accggagggc aagctcctcg gtctgcgcga
     6961 gcagctccgc gaagtagaca cgcagatcca cctgcgcgtc gtacagcgga tcgcccgccg
     7021 ccgccgcaac cagatcgctc agggcgcggt atccctcgac tatggcggcg gcccgctccc
     7081 ggccggaggc gctcagggcg gcattcagac tcgccaggta ctgccggccc agacgcagca
     7141 gcgtcgtctt cttcggtccg tcggcgacca gctcttcaag gacggctagg aggcggttcc
     7201 ccggcacgcc ggccaggaaa ttgacttcgg cagtccggct gccgacccgg ccgaccacct
     7261 ggcggtatcg gaccagttgc ggctcggcgg ctgcgcgcgc ccggtaatcc cgccccagga
     7321 gctgcgggat ttcgcctgcg agacgatcgg cgtagcggac cgccagctcg cggcggctca
     7381 ggaaaaccgc ggccgccgaa tcgagcgcct cattgggatc ggtcacctgc tcgtagatct
     7441 tcagcacgtt gaagcggcgg tcggccgagg gttcggccgc gaggatccgc tcccggaggg
     7501 cgttgtaacc cccctggccc cgcaaggcct cgaacagaac gatgtccggg taccgctcgg
     7561 aaaacagcat cgccgtcgcc gaatccatcg cctcccggtc ccccggcggc agcaggagtg
     7621 tgtgcacgta gagctcgcgc tcgggaaggc cggcggcggc cgtgcgatag ctggagcgct
     7681 cgtccaggag caccacgtcg atccggcatc cgtgctcgtc gttgagatag tacaacgtcg
     7741 gccagtcgat gcgctccggg tcgtcggaca catagaggac gcgcacgtcg ctgagaacgt
     7801 aaccggccgg atcggcggcg cgggccgcgg gcggcaccgc gatcgccgcc agcgccgaca
     7861 ctgccgcgga gagaaacgcc cgccgcaggc acgcccccga catgaaccgt agggtggtca
     7921 tcgcccggaa tatactagcc gccggccggg gcctgtcaaa tttcaaatgc aggccgcctc
     7981 cctgtccgct cagcgtatta tcgtctcatc ctccggccgc catgccggat cgacgagaca
     8041 gaggaaggtg agcggttccg cgccggggtt ctcgatccac tggaccgtcg ccggcgggac
     8101 ttcgatcgta tcgcccggct ccaccagcgc cagctcctcg ccgacataca ggatcccctt
     8161 ccccccgagg atatggtaga cctcgtcggt ggtgaggacg tgcgccgcgg tgcgttcccc
     8221 cggcggcacg gtcccatggg ccagcgagta gcggccctcg aacgggtacg ggcggtcggg
     8281 gtggaccagt tcccggagcc gggcgccgtc accggcagtg gtttccggac agtcgagcag
     8341 tttatgaatg cgcatgccgt cttccttagg ctgcggcggg gccggccgcc tcggacagac
     8401 cccttccagg ttctcatgaa agagccgcca ggcgcgcgcc tgacggcggt cgattttcgg
     8461 agtcggcgtc gacttacaga atctggttgt cgcggagttt ccggaaaagc ctctcggcga
     8521 tctcctccgg cgtctccccg tcgatcctct cccccttggg gcgcgggggc ggcggcgcca
     8581 ctttcatcgt cgcggtcgcc gagtgggccc ccaccgtcgc gggatcgata ccgaggtcgg
     8641 cggcgttcca ggccgcgacc tccttcttct tggccgccat tttccctttc agcgacggca
     8701 ggcggggctc gttgatctcc ttgacgactg agaggacggc cgggagagcc agctccacga
     8761 catcgtagcc gtcttcggtg gttcgttcga cgacgatctt gccgccgtcg acgctctgca
     8821 ctttcttcac gaacatggcc tgcggcaggt ccaggagcgc ggccacggcg ccggggacct
     8881 gggcggaatc ggaatcaatc gcctgcttgc ccgcgagaat gagggtgaag cggccgagct
     8941 tcttcagccc ggcagcgaga attcgggcta ccgcctgcgg gtcggacccc tcgaaggcgg
     9001 gatcggacag caggtaggcc tcgtcgacgc cgagcgccag acaattgcgc agcgccgcat
     9061 cggcccgctc ggtccccacc gtcatgaccg ccaccgtccc cccgtgcttc tccttcaacc
     9121 gcagaccctc ttctacggcg tactcatcga acgggttgac cgtccccggt ccctgcggca
     9181 attccacctt gttggcgctc tcgttgaccc tgatgagggc gatttcgggt acttgcttga
     9241 tcaggcagac gatgtccatg ttgactccac tttgcctagc ttcacgggtc ttcctcagag
     9301 tcgaatatca tggctggggg acgaaaagtc aagggcgggg gaggcaggaa aaagcccgct
     9361 ccggggcggg ctttttccgg tactggtaag ggaggtctag cggcggcggg cggtcttccg
     9421 ccccgcggtc ctggccgccc cgccgcgccg tccggcgaca gtgcgccgcc gggccgcggc
     9481 cgatttcctc agcttcaagg tccggatctt ggctttcacc gacgcgagcg agcggccgag
     9541 acgccgcccg acctcgtggg ccgtggtctc cttgtagagc cgcacgagtt cctggacctc
     9601 tccctgcgtc caagccttcg cccccggccg cacggcgcgg cgcgcggctg ttttcttcct
     9661 tgttgccttg gtagccttgc gcatggctgt accctcctgt taaggttgtg gttccgcagt
     9721 caacacggcg atgaccatag ctatgctcgc ccgttgctcc ctcgtcctcc gagaaccctt
     9781 attagtatta cgcaccaagc cgcggatcgc gcaaaaaaaa ctccccggcc ccccggcccg
     9841 cggcgaagga accctcctcc ggggaccgtt cccgacggcg ccggcgttcc gttcagtcgg
     9901 cggcgcggcg gacgtggagg agcgccgcct cggcggcgcg ctgctccgct tccttcttcg
     9961 agtgcccgga accggatccg atcgtctgcc cgttgagcgt cacctcgacc ctgaacacct
    10021 tctcgtggtc gggaccggtt tcggcgacca cgtcgtactg cggcatcccc tgcccccggc
    10081 tctgcaccag ttcgagcagc tcccccttga agttctggcg ggaggcgtcg gtcgcgatga
    10141 ccggctcccg ggtgtagacg aggcggatga cgacgtcgcg ggcggcctcg aacccgccgt
    10201 cgagaaagac cgccccgatg atcgactcca cggcgtcgga gacgatcgag gcccgctcgc
    10261 ggccccccga gcggtcctcc tccggggcca ggagcagatg gcggttgaga ccggtgctga
    10321 cgccgatttc ggcgagggtc gcctcgctga cgaggagcgc cttgcgcttg gtcagttccc
    10381 cctcgcggag atccgggttg tcctggtaga gccgctcggc gatgacgagg ccgaggacgg
    10441 aatcgccgag gaattcgagc cgttcattgg acaggtggta ctggtcggcc gcgtgggcga
    10501 acgaccgatg ggtgagcgcc agctcgagga cagccgggca gcggaagtgg tagccgatga
    10561 gctgctgcac ggcggccgga gcggcggcgc gccgctgcgc ctggctggga gagaaaaatc
    10621 gtctgaggcg gtcccaaaca cccatcacgc agagccgttg actgttccga ccaccagcga
    10681 cacgttgtgt ccgccgaagc cgaaggagtt ggacagggcg tggcggatcg tcaccgcccg
    10741 cttgccctcc cggacgtaat caagatcgca ctcgggatcc ggcgtgtcga tgttgatcgt
    10801 cggatgcacg acgctatccc ggagggatag cacgcacgcc gccacttcga gcgcgccggc
    10861 cgccccgagg aggtggccgg tcatggactt ggtcgagttg cacgggatct cgtaggcgcg
    10921 cgggccgagg acctccttga tcgcgcgcgt ctccgccgta tcccccagga tcgtcgccgt
    10981 cccgtgcgtg ttcacgtagt cgatctgctc gggcgccagc ccggcgtcgc ggagggccat
    11041 ggccatggcc cggcgcgccc cgaggccctc ggggtgcggc gcggtcatgt ggtgggcgtc
    11101 gccgctcatt cccgccccga gcagttcggc gtagatgcgg acgccgcgcg cccgggcgtg
    11161 atcgagcgac tcgagcacga ggatggccgc cccctcgccc atcacaaagc cgtcgcgctc
    11221 cgcgtcaaac ggccgcgagg cgcgctccgg ctcatcgttc cggaccgaca gcgccttggc
    11281 ctggcagaaa ccggccagcg ccgtccgcgt gatcgtcgct tcggccccgc ccgtgaccat
    11341 cacgtcggcc tcgtcgcgct ggatgatgcg gaaagcatcg gcgatcgcgt gggccgacga
    11401 ggcgcaggcg gagacggtgg cgtagttcgg tccgcggaag ccgtaccgaa tcgacaccag
    11461 cccggcgcac atgtcgatga tcatcatcgg gatgaagaac ggcgacacct tggccggccc
    11521 gccggtctcc tccatgaggg agtgctgccg ctcgaacgtg ctgatgccgc cgatgccgga
    11581 gccgatgacg acgccgatcc ggtcggggtc gagccgcccg tccttcagcc cggcgtcaac
    11641 cagcgccatg tcgctggcgg ccacggcgta ctgctccgag aggtccatcc ggcgcgcctc
    11701 tttcttctcc aggtagagct ccggattgaa gtccttgacc tcggcggcga tctgggtcgg
    11761 gaacggctcc gggtcgaacc gcgtgatccg ccccgccccc gatttcccgg ccaggagcga
    11821 ctgccagaac gcctcgagcg tattcccgtt cggggagacg acccccatgc ccgtcacgac
    11881 aacacgtttc ttcatagttg aggttccgtt ccttccgtga acggcctgcc cgccgcctac
    11941 ttggccttcg ggctgtgctc cgcgatatag gtgatggcgt cgcctaccga gacgatcttc
    12001 tcggcatctt catccggtat ctcaatcgag aattcctctt ccaacgccat caccaactcg
    12061 atcgtgtcga gcgaatccgc gccgaggtcg tcgacgaact tggctttctc agtcacctgg
    12121 gaagcttcga cgccgagttg ctcgacgatg atttccttca cccgatcttc cagttctgcg
    12181 gccatagtag cacctcttcc tgagtatggt tgtatgtctt cagtccagtt cacatggcca
    12241 tgccgccgtc gacggcgatc acgtggccgg tgatgtaggc ggccgcgtcg gacgccagga
    12301 acatcacggc ggcggcgaca tcgtcgggcg tgccggcccg cttgatcacg ctgcgctcca
    12361 aaaaccgggc gcgcgcttct tccggcagca cccgggttat gtccgtttca atgaacccgg
    12421 gtgcgacggc gttgacggta atgccgcgcg cccccagttc tttggccgcg gatttcgtca
    12481 atccgagcag tccggctttc gacgccgcgt agttggtctg accggcgttg ccaaacaatc
    12541 cgaccacgga gctgacgtta atgatgcgcc cggagcgctg cttcatcatc accttcgcgg
    12601 ccgatttcgt gacaagaaaa gcgcctttca gattgatgtc aagcaccatg tcccagtctt
    12661 tttcgtccat ccgcaccagg agcgtgtcgc gggtcacgcc ggcgttgttg accaccacgt
    12721 ccacggaacc gaaccgctcc tgtgcggccg cgatcagggc ttcggcgtcg gcgctgctgg
    12781 tcacgtcggc ccgcacgccg atggcctcgc ccggcatggc ggcggccgcg gccgccagcg
    12841 cggcgtcatc gacgtcgctg atcaccacgc gggcgccggc gcgggcgaac cgctcggcga
    12901 tcgcccgccc gatgccgcgg gcggaaccgg tgaccacgac cgtcttgccg ctaaaatcca
    12961 tcatcttccg cctccctcag gggcgctgca gcgcgcccag cgcctcgatg tcggccaggg
    13021 tgtcgagatt gaccgacgtc gtcaggttca tgtcacgtct tgccagtccg gtgagcactt
    13081 tgcccggacc gatctcatac acggtcgtca cgccctccgc cgccaggcag gccatcgtct
    13141 gcgcccactt caccggcgcg gtcacctgct cgatgagcag gcggcgaatc gttgcaccgt
    13201 cagtcgccgg ctcggcggtc acgttggcga tcacggcgcg ggcgggagcg gcgatcgcca
    13261 gatcggcgag atacgtccgc agtccctcgc gggccggctc catgagcggc gagtggaaag
    13321 cgccgcccac ctcgagcagc atcgcccgct tggcgcccgc gcttttggcc agctcgatcg
    13381 ccttctccac tcccggccgg gaccccgaga cggcgatctg gattcccgag ttgtaattgg
    13441 cgggcacgac cacgcccgcg gcggcggcct cgcggcagac ctgctcgacc tgcgcggcgc
    13501 tcagccccat cacggcggcc atggttcccg gctgctggcg gcaggcctcc tccatcaggt
    13561 gggcgcgccg cacgacggcg cggatggcct gctcgaacgt cagggcgccg gtgaccgcca
    13621 gggccccgta ctcgccgagc gaatgcccgg cggcgtaatc gaacgccggc cggccgtccc
    13681 ccagcaccgt cagcgccgcc agcgagtgca gcaggatcgc cggctgggtg aaccgggtgc
    13741 gcctgagatc ctcggccggc ccctcgaacg agaggcgggc gatatcgaac tgcatggcgt
    13801 cggccgccag ctcatacagg cggcgcacgt cggccgaggc ctcatacaaa tcccggccca
    13861 tcccgacgta ctgcgaggcc tggcccggaa agaacaacgc tgtcttgctc atactcacca
    13921 cctcacgaga gcggagcccc agatcaggcc gccgccgaaa gcgacaagga ggatgtggtc
    13981 gccggggtgc aacatccccg cgcggttcgc ctcgtccagg gccagcggca ccgaggccgc
    14041 cgaggtgttg ccgtagcggt ctatgttcag gtacacccgg tccatggata ccttcatccg
    14101 cttggccagg gcctcgatga tccgaatgtt ggcctggtgg gggacgatga gcgcgatatc
    14161 gttcgagtcg atgcccgcgt cggccaccac ccgctcggcg gcgttcgcca tctcccgcac
    14221 cgccaccttg aacacctccg aaccgttcat caccagcttg tccgaaccgt cgaacgggaa
    14281 ctccggcgtg taggggaagg ccgtgccgcc gactttctgc cacagcaact ggctgtaccg
    14341 gccgtcggag cgcatgaacg acgcgagcag gccgcgcccg ctgtcgtcgc cggtgaccac
    14401 ggccgccccg gccgcatcgc cgaagaggat gcaggtgccg cggtcgttcc agttggtgat
    14461 ggaggagagc ttttcggcgc cgacgaccag ggcatttgtg tgctggccgg aggagatgaa
    14521 ggaggaggcg atcgtgagtc cgctgatgaa cccggtgcag gcggccgcga tgtcgaaggc
    14581 gacggcattc accaagccga gccgctcctg cacgaggcag gccgtcgccg gcacccggta
    14641 atcgggcgtc accgtgccga ttatcaccag gtcgatctgg tcgggggcca gatccgacat
    14701 cgccagcgcc gcgcgtgcgg cctccgtcgc catttccgac gtcgccgtgc cgttcgccgc
    14761 aatccggcgc tcccggattc ccgtgcgggt gacgatccac tcgtcggagg tgtcgatggt
    14821 cttttcgaac tcagcgttcg tcaggcgccg aggcggtaca tacgacccag tcccgattat
    14881 ccttgctttt atttgcccca ttcgtgtttc ccagttgatt cgtgagtagt tcttcgtgga
    14941 tgcgctcccg gattcccttg gcggccatct cgtaggcggc gaggacggcg ttctggatgg
    15001 cgagcgaact ggacgagccg tggcagatga ttacggtgcc gtcgacgccc aggagcggtg
    15061 cgccgcccga ctgcgcgtag tcgaactgct tggccatgcg gcgcaaaaac ggcagaagca
    15121 ggaaggcgcc gatgcgggag aaaatgttgg tctggatctg gcgctgcacg gccttggcga
    15181 gcatcggctg caccgactcc gcgaacttga gcaggatgtt cccggtaaac ccgtccgtca
    15241 cccctacgtc gaccgtcccc gacagaatgt cccgaccctc gatgttcccg acgaagttga
    15301 tgtgcgaggc cgccagcagc ttgcgggccg agaacaccag gtcgttcccc ttactccgct
    15361 cctcgccgat cgagatgagg ccgacacgcg gggcctcaag gttgaacatg atgctcgagt
    15421 agatgctgcc catgaccgcg aactgcgaca ggtgggtcgg cttgcagtcg acattcgcgc
    15481 cgcagtcgag cagcacgagc ggccggccgg tggaggtcgg aaacatggtc gtgatggcgg
    15541 ggcgcatcac gccctcgatg cggcccagcg ttaacagtgc ggtcgccatc accgccccgg
    15601 tgttgcccgg cgacacgaac gcatcggccc gtcgctcgcg gacttcccgg aggccgaccg
    15661 ccaccgagct gtcgcgcatg cgggcgccat cggtggccgc gatggtcatg ggaacttcgg
    15721 tctcggcgtg gagaaccccg accgcggggg ggacgttgac cgtgccggcg aggacgcgat
    15781 cgatggtcgg gcggtggccg acgagcacga cgcgcgcggc cggaccgatc aggcgggcgg
    15841 cctcgatccc gccggcaatg atcccctcgg ggccatggtc tgctcccatg acgtccagca
    15901 gaatcgtgtg tgcgctatcg gtcgccatcg actcgactcg ttgccggcgc ggccgcgcgc
    15961 cggcgacatc cgcagcgtat tacgcttctt tcggctgaat gatcgggcgg ccgttgtagt
    16021 atccgcagtg cgtgcacacg tggtgcggaa gccgcggctg gtggcagtgc gggcactcgc
    16081 tcacgttcgg cgcgctcaac ttccagtgcg tgcggcgctt gcggccgcgc gtgcgggagt
    16141 gtcgtctctt gggaagtggc atctcgtttc ctctgtcaat cgctctcgcc gcgcaggtct
    16201 ctgagcttgt tccagcgcgg gtcgacgtca tcgaacgcac agtcgcaggt ctgttcgttc
    16261 cggtttattt tgcacttcgg gcagagcccc ttgcagtcct cgcggcacag cggcgtcatg
    16321 ggcaaagcca caatgaccgc ctgccggacc ggctcgccga ggtccacctc gagcgaccgc
    16381 ccttcgtaga acacatactc ctcgtcgtcc tcggcctcgg cgtgggcctc gacccaggta
    16441 tcgtacgagc acacgatgaa atcggtctct ccatgcagtt gctcctcaaa ccggaccaga
    16501 cagcgggagc actcgacccc aacctcggcc gcgacctgcc cctggcagaa gaactcctcg
    16561 ccggacttct gaatatccag gtcaaccgtg acgcggccga cctcaacaac acccgggtag
    16621 tctacgggca aggtccccgg ctcggcctct aaacagacat gggcgggaaa agtttcgaaa
    16681 tcccgcagta ccaatttcat agccttgtag tgtacaggac cggagccccc aagtcaaggg
    16741 agaaaacgtc ggcctaaccg accgctgtac ttggccttac cgcgtcggtc gggccccgcc
    16801 ccctggcctc ccgccgccgg ccgctcagcg ccgcgcctcg tagacgcgga ttcggttgcg
    16861 cgcccgctcg atcgcgtccg gcaggccgcc gaggtcggtc cccggcttcg cctgcgccgc
    16921 cgccagcgcc tgctggttcc gctcaagagc cgcccgcgcc cgggcgatgt cgatctcctc
    16981 cgcccattcc accgcgtccg ccagcagcgt cgccgtgttg tgcgacacct cgaggaaccc
    17041 ccccgacacc gccatcaccc gcaccttgtc ggcggcgtcc cggaactcga tcttccccgg
    17101 cttgagggcg gtgatcagcg gggcgtggtc ggagaggatg ccgaggtacc cctcgctccc
    17161 cggcaccacc agcgaccgga tttcggcctc gaaaaagact ttctcgggcg tgactatcga
    17221 gagtgtgaac atcgtccccg ccgcttcact tcctggactt ctcgtaccgg tcgagcactt
    17281 cgtcgatgcc gccggccatg tagaactgct gttcgccgac gtgatccagc tcgccgtcga
    17341 tgatcatcct gaagccccgg atggtgtcct cgatcttcac gtacaccccc ttgcggccgg
    17401 tgaaggcttc cgcgacaaag aacggctggg acagaaagcg ctggattttc cgcgcccggg
    17461 acaccgtgat cttgtcctct tccgagagtt cgtcgatgcc caggatggcg atgatgtcct
    17521 ggaggtcttt gtagcgctgg aggattttct gcaccgcccg cgcgacggcg tagtgctcct
    17581 cccccacgat ctgggggtcg aggatgcgcg aggtcgaatc gagcggatcc accgccgggt
    17641 agaggcccag ttcggcgatc tggcgcgaga gcacggtggt cgcgtcgagg tgggcgaagg
    17701 tggtcgccgg cgccgggtcg gtcaggtcgt cggccggcac gtaaatggcc tggaccgacg
    17761 taatcgaacc ggctttggtc gaggtgatcc gctcctgcag accgcccatt tccgtgccca
    17821 gggtcggctg gtagcccacc gccgagggca tgcgcccgag gagcgccgag acctcgctgc
    17881 ccgcctggac gaagcggaag atgttgtcga tgaagatgag cacatcctgg ccctcttcgt
    17941 cgcggaagta ctcggccatc gtcagtcccg acaggccgat ccgcaggcgg gcgcccggcg
    18001 gctcgttcat ctgtccgaac accatggccg tccggttgat cacccccgac tcggtcattt
    18061 ccagccagag gtcgttcccc tcgcgggtcc gctcgccgac cccgcagaac accgagtagc
    18121 cgccgtgctc ggtggcgatg ttgtggatca gttcctggat gatgacggtt ttgccgacgc
    18181 ccgcgccgcc gaacatgccc accttgcccc ccttcacgta cggttcgagc aggtcgatca
    18241 ccttgatccc ggtctcgaac atctccgcct tggtcgcctg ttcctcgaac ggcggggcgg
    18301 ggcggtggat gggcagccgt ttgcgcggct ctttgacttc tcccttggag tcgatcggcc
    18361 gtccgagcag gtcgaacacg cggccgagga cctggtcgcc gaccggcacg gtgatcgggc
    18421 tgccgctgtc gatggccggc atgccgcgca ccaggccgtc ggtcgaggcc agcgccacgc
    18481 accggacgat tttgtcgccg acgtgcatgg aggcttcgac agtcagatcg atatttctcg
    18541 cctgatcgac aattcggatg gcgttgagga tgttcgggag cgcgtccgag cggaactcgc
    18601 agtccaccgt cggcccgatt acctgcacca ccctgccaat gttttcagcc attgagataa
    18661 ctccgcatct atgtcacatc attctcttcg cgtgaatccg ccccggcctc atcctttcag
    18721 cgcctccgcc cccgagacca cttcgagcag ttctttggtg atctgggcct gccgcgcctt
    18781 gttgtagtcg agggtgagcc ggtcgatcat ctccccggcg tttttcgtcg ccgcgttcat
    18841 ggcgatcatc cggctgccca gctcgctcgc gaacgactcc acgagggcgg tgacgaacat
    18901 cgtcgtcgcg tagctcggca tgagcgccgc gtagatgtgc tcggcatccg gctcgaagat
    18961 gtagtcggcg ccgcccggcg ccgccgcccc gaccgccggc ggccggatgg ggagatagct
    19021 ctgcagcgtg atccggtact tgctgagcga gagaaaccgg gtgtagagca ggtcaatgcg
    19081 gtcggtctcg ccgctcacaa agcggtgggt gaggaagccg acaatgtcgc gcgccttgcc
    19141 gtaatcgagc gtcgtgttga aaccggggtg cgccccgacc accgcctgcg ggcggcggcg
    19201 gaaatagtcg taccccttct tgccgatgac gaccagttcg gtcgcgaccg ccgtgtggtc
    19261 gcggagccag gcgagcgtgt cgcggatgat gttggcgttg taggagccgc agaagccgcg
    19321 gtcgcccgtg aaaagcacga gcgtcctctt cctgaccggg cgctcctcga aatacgggtg
    19381 gacaatctcg ttactcgatc ccgccgccag gtgggccagc atctcttcca acttgtcggc
    19441 gtacggccgg gtctgttcga ccttctgctg ggcccgccgg agcttcgccg cggccaccat
    19501 ctccatcgcc cccgtgatgc gccgggtcga gcggacggag cggattctct tcttgacgtc
    19561 gcgcagtgtg ggcatgcgtc gctacacctg tttgtgcggt cccggtccgg ccgccgccgg
    19621 ggcggcgccc ggcgccgagg ctgcaaactg cgccttgaac ttcgccaccg ccgcctgcag
    19681 ctctttctcc gtcgactccg aaatcttctt ctctttgctc aggttgtggg cgatgtccgg
    19741 gaattctttc tcgcagaagg cgaggaattc ggcctcgaac gtcttgaccg cctcggccgg
    19801 cacgtcgtcg agatacccct tggtccccac ccagatgatc atcacctggt gctcgaccga
    19861 catcggcacg tactgcccct gtttgaggag ctcgaccatt ttgcgcccgc ggttgagctg
    19921 gcggagcgtc gcctcgtcga ggtccgaccc gaactgggtg aaggcctcga gctcgcggaa
    19981 ctgggcgaga tcgagcttga gcgacccggc cacctgcttc atcatcttgg tctgggcgtt
    20041 gccgcccacg cgggacaccg agatgcccac gttgatggcg gggcggacgc cggcaaagaa
    20101 cagctcgccc tcgaggaaaa tctggccgtc ggtgatcgaa atcacgttgg tcggaatgta
    20161 ggccgacacg tcgccggcct gcgtctcgat aatgggcagg gccgtgagcg acccgccgcc
    20221 gtgtttttcg ttcagcttgg cggcgcgctc gagcagccgc gagtgggtgt agaagatgtc
    20281 gccggggtag gcttcgcggc ccggcgggcg gcgcagcaag agcgacagct ggcggtacga
    20341 ctgggcctgc ttggacaggt cgtcgtacac caccagggcg tgcttgccgt tgtacatgaa
    20401 ctcctcgccc atggcgcagc cggcgtaggg ggcgatgtac tgcagcgggg ccgggtcggt
    20461 ggcggtcgcc gccacgaccg tcgtgtactc catcgccccg tacctgcgca gcgtctccac
    20521 cacctgcgcc accgtcgagg ccttctggcc gatcgcgacg tagatgcagt agacgtcgga
    20581 atggcgctgg ttgatgatcg tgtcgatcac gagcgccgtc ttgccggtct ggcggtcgcc
    20641 gatcacgagc tcgcgctggc cgcgcccgat cgggatcatc gagtcgatcg ccttcaaccc
    20701 cgtctggagc ggttctttca ccggctggcg gtcgaccacg cccggcgcgt ggccctcgat
    20761 cacgcggaac ttgtcggtga caatcggccc cttgccgtcg agcggctggc cgagcgggtt
    20821 caccacccgc cccagcaggg cctcgccgac cgggacgctg gcgacgcggc cggtccgccg
    20881 caccgtgtcc ccctcgtgaa tgtgcgtatc ggagccgaag atggcggcgc cgacattgtc
    20941 ttcttcgaga ttgagcacga ggcccatgat gtcgccggga aactcgatca gctcggacat
    21001 ctggacatcc tcgagccccc agatgcgcgc gatcccgtcg cccacctgga gtacggttcc
    21061 gacggattcc atttcgagcc tggtttcgta cttctccagc tctttcttga tgaccgatgt
    21121 aacttcttca gggttgagac ccatcttcgt actcctgtct taaccgcacc gccatcgccg
    21181 catccgggcg gggccgcgcc gccgccccgc gcgcgtcgca tggggcggcg cctgcggcgg
    21241 tcagttcact ttcaccttca aaagctgctc ctcgatcagc cccagcccgt gccggatcga
    21301 gccgtcgata atctcgttgt gcaaaatgac gatcaggccg ccgaggatgg ccggatcgat
    21361 ccgcacctcc agccggatct gcaggcccgt cttggccgac agccggccga ccaggttctc
    21421 ccgctccggg ccggacagcg gcacggccga gatcacccgg gcccgcccga tccccttggc
    21481 cttctccacc cgctcgacga actcgtcgat gatctcgacc aggtagttta tgcggtgctt
    21541 gccgaccagc acgacgagga actcgacaaa cagccgttgc agccggctgc cgaacaccgt
    21601 cctgagcagt tcgatcttgt gctcgtcgag cacctgcggg gcggtgagga aattgagcag
    21661 ggtccgatcg gcgcggacaa cggtccgcag gtcggccatc tggctgtacc cggcgtcgag
    21721 cagtcccttc tcgcgggccg cgtcaaagag cgcgtgggcg tagcgttgag cgacttcctg
    21781 tgccagcata gccgcctcag gccttttcga cgctgtcgat gaagcggccg atcagctggc
    21841 ggtgccgggc gtcatcgagt ttttcgcgga gcagtttttc ggcggcgccc atggtgatgg
    21901 ccaccatctg ctctttgagt tcgacccgcg ccttggccac ctcgcgctcg agatcggtgc
    21961 gggctttctg ggcaatctcc cgggcctcgt cgcgggcctg ggccttgaga tcctcggcga
    22021 tcctcttgcc ctccgtcacc gcctcgacca gtttctgccg ccgctccgat tcgatctgct
    22081 tgagcttggc ctcgtactcg gccgccatcg cggccacctt gcccttctcc ttctcgatcg
    22141 tctcgaattc gccggcgatc cgggaccggc gctcctccat gatgttcatg agcggtcccc
    22201 aggcgtaccg cttgaggatc cacaccgtga ggaggaaccc gagcgcgtgt gtcagtattt
    22261 gctgcagttc gaaattcata gtatcaccaa gctcctaggt tgagaccgga agccggcgcc
    22321 ccgacacacg ttttcaaccg ccgattttgc cgctggccag gatgtagacg atgaacacat
    22381 agatggtgag cgcctcgatg aaggcggcac cgatgatcat cgccgtctgg attttgcccg
    22441 ccgcttccgg ctgccggccg atcgcttcca tcgccgaacc cacggcgcgc ccgatcccca
    22501 gaccggaccc gatcgccgcc aggcccaccg ccaggggagc cgccagaccc aacatcgcct
    22561 gataatccat cacatcctcc ttttaccgct tgaaaccgta tggtccatcg ttcgttctgc
    22621 cgcgtgtgcc tcagtgcgcc gcctcgtcgt gcggcagcat gaggagtatg taaatcgtcg
    22681 acaacagcgt gaacacgagc gcctggatcg tcgacagcaa gagccccagg aaatagaacg
    22741 ggatctgcag cgggacgccc accggggaga agctgatcag catgatgccc agtgtcacga
    22801 agacggcgat gagcgtgtcc tcgccggtga tgttcccgaa cagacggagc gacagcgaga
    22861 acggcttggc caactcgccg ataatgtgca gcgggaacat gatgatcacc atcgaccacc
    22921 ccaccgcccc gaccgggctg cccgccaggt ggtacaggta accgccgatc cccagcctcc
    22981 gtatccccgt ccactgcgca tagagaaaca ccagcaccgc catcgaggcc gtgatgttga
    23041 tgctcgtcga aggcgagtgg cccagcggaa tcatcccgat ccagttgagc gcgagaatat
    23101 agaaaaagag cgtcccgaga aacggaaggt actttcgggt ctcatggccg aggatcgagt
    23161 agatgaagtt gtacatcgac tcgacgcacc actccagaaa attctgcagc cgtccgggta
    23221 tcatctgccg ccggcggtag acggacatgg cgacgattgt gaaaaagatc gcaaccacaa
    23281 acgcgaaaaa gacattgatc cagtgttcga ggaaccgcac ccaggccacg tcctcgatgc
    23341 cgaaggccca accgatcagc cagatgaggc tgggcaggtg cggcggtccg gcatgcgccc
    23401 cgcccgcggc atggccgtcg gagacggcgg cgccatggcc ccccgcctcc gccgacacgc
    23461 ccgccgccag cgcgggcgcg tacttggccg cggtcgtggc gacgactgtg agatagtagg
    23521 cgagcattta caccgccctc tgcagttgcc cgcgtttccc ggccgagcgg tcggcgccca
    23581 agagcgcgcg ggcggccacc ttgagcacca tcaccgccag gggaaccgag aacccggcca
    23641 gcagcgccag ggggccgaag agcggcgtcg tggcgagaaa gtagccggac gcgtagagca
    23701 gcggaaactt caccagcgcc acgccggcca cctccatctt gttggccccc ccggggcgaa
    23761 tcgccgcgcg caccaggcgc gtcaacagca tcaggttcac catcccccac acgcctcctg
    23821 acagcaccgc caccgtcggg tagatcccgg cgtagtagat gccgaagggg acgaagatca
    23881 ggagcaccag ccccgtcgtc ttcagcgtcc gattgatgaa accgaggtcg atcgtctcat
    23941 tcatggcgtt tttccgaatc gtccgccgac gccttccgca ccacctggac aatctccagc
    24001 gctgcggcgg cgacgcccat cagcaccccc agggcggcga ggtacggctc cgtccccagc
    24061 ttccgatcgg cccagttgcc gatcagatac ccgacaagca ccccgcccag caaaaggaac
    24121 gggacggctg tcagcaggcc tatctggcgg aggtcccggc cagactgatc gtcgggaccc
    24181 gacgacaaca atcccatctc agcctgcctg cgaaaattca ggatgccaat atatcccggt
    24241 gcgcactact gtcaactgat ttttttggcg ttgtgcggcc cgtttccgta tgagaaagga
    24301 tctttcagtc acaattcccc tctgataatc ggctatttcc aaacgacata ctattgttca
    24361 acctgggtcg ccttacgcca caatgagacg accgtgcgcc tgccggtatg tgacaaatgg
    24421 cgcaagcgga gacccgcgcg aaagaaccgt ccttgagaat aatttcacta aatgccatac
    24481 aacatcaggt aaatggccac cccgctcacc gagacgaaca gccaaaccgg ccacacccac
    24541 cgcgtgatcc ggacgtgccg gtcgaaccgc cgccgccacg cgtgccggac cgccagcagg
    24601 atgaacggga ccatcacgcc cgccaggatc acgtgcggga tgaggatgcc gaaatagacc
    24661 ggccgcgtcc agtcgtgccg cggatagggg accgagccga cccgggcgtg gtagaccagg
    24721 taacttgcga gaaacagcgc cgacgtcgcc agcgcggcga gcatgaacct cctgtggatg
    24781 tccgcccgcc cccgtttgac cgccgcgtac cccgccagca gcagcagcgt gctcgtcccg
    24841 ttgaggacgg cattgaggga ggggaggtcg gcggcgctca tggcaggtcg tccagcagtt
    24901 cgcggaggtg cgtgcgcagc acgtccacgc tggccgcatc cgtccccgag tagtacgccc
    24961 ggatccggtg ccggccgtcg accagcgcga accgcgtgct gtgattgccc ggaagatcat
    25021 ccgccgcgag catgaagtag gtctccgaga tgcgcacgat cgagtcgagt ggaccggtga
    25081 gaaacaccca gcgccggtcg ctgaccccga accgggcggc gtagtcggcc agccgggccg
    25141 tcgaatccgc ctccgggtcg accgtgatcg acacgaactg cacccggtcg gtctccgcaa
    25201 aggcccggta gaggtcggcg aagtggccgt tcatgatcgg gcagggtccc cggcagccgg
    25261 tgaagaagaa ctcgaccacg ctgagcttgt cggtcaggtc ggcatcggtg aacggctcgc
    25321 cgaactggtc ggtcagcgtg aacggtccca ccgtgccgag aacgggcaac tcggaccgcg
    25381 ctttgtcggc ctggtagagg gtgacgaggg cgaccccgcc gagcaccgcc gccagaatca
    25441 ccccgatcgt gataatcttg ctcatcctct catctccccg gccggcaccg gtccgggctg
    25501 ccgcgcggcg gccagggcca gcaggagcgt gccgatatag agcgaggcga tccacaggtg
    25561 gaagacctgg acgaccggcg ggatgtccca gaaaatgaac gtcagaccgc tgaggatctg
    25621 caatcccacg agcaccatgg cggcgaccgc ggcctgccgc accgcgggcg cgctcccgcc
    25681 gggggcgcgc agcaacccgc gcccggtcca ccaggtcacg gccgcgacca cgattcccag
    25741 cgccatgtgg agatggctca ccgccccgat cgccatgatc gctttcgccg ccgaccaggc
    25801 cggatggtcc tcgcgcatcg cctcgagccc ttcccgcacc cgcgtgccga gcatcacctg
    25861 aacgatggcc agcccccaga gcagaaggag ccggcgctgc gcccaggcgg ccaggccctc
    25921 ccccgcctcg cgcgggtgga cttgccgata ggcgcccgcc gccgcccaga tgaggaggct
    25981 gacaatcacg agcgcgagga ccgtgtgcac cgtcactacc agcggcgcga gctccgaggt
    26041 gatgaccgct ctcccctgcc acccctggag cgccgtgagc aggccggccg cgaccgccgg
    26101 ccacaggatc ccgggcgtcc ggcgcgcccg cacaagcgcc agcacggcgg tggccgcgat
    26161 cagcaggccg actatcacgc ccgaaagccg gttggtgtac tcgatccagg cgagcgtgaa
    26221 attgaaccgg ctcgggtcga tgtcgggggg gagctggctg acgtcggtcg ggggcatcca
    26281 gcggccgaaa cacttcggcc agtcggggca gcccagcccg gcgccggaca cgcgcaccag
    26341 gccgccgatg aaaatgagca cgtaggttgc gatggtggat accagcgcca tgcgccagag
    26401 cgacttcata gacccttcca cttctaacgg ccgccggctg cgccgcgccc cgggccgcag
    26461 cgctttcccg atactaccgc cgattcaatc ggggcgcaag cttttcctgc ccggccggcg
    26521 actcagcccc ccgcggccgc cgtatccgcc gatggcgccg cctgccgata aatgaccgcc
    26581 tcggggatga tcggcgattg cttctcggga tccacgtgcc cgcgggtcat cgtgtcggac
    26641 atcgtcagca cgatgaacac ggccaggaag accacggccg tgatgaacac cgccgcgtac
    26701 actttgctgt cgtacttcag gtgcatgaag aacagcgcca ccaggcccgc tttaaccgcc
    26761 gccaccgcca tcgccaccac caggttgagg gccccgaagt ggaaccgggc gatgaagacg
    26821 gtgagtgcgg tcagcaccag gagcgctgcg aagacgccga agtagacgcg cagcgggaga
    26881 acgtgttccg tatgtacgtg tgtcgcgctc gccatagccg ttcaccccac gagatagaac
    26941 aagggaaaga ggaaaatcca gatcaaatcg accaggtgcc agtacaggcc ggtgagctcg
    27001 accggcgtgt agtactcggc cgagaaatgg tttctcagcg tgcgggcgag cacccaggtg
    27061 atgacgccca tgccgaccag aacgtggatg ccgtgcagcc cggtcatgag gaagtacacg
    27121 ctgaagaaca cgtgcgggtt ggtcccctcc atgccctgga acgtgtagaa cttgcccggc
    27181 agctgcccct cgtgaaactt gtgcgcgtac tcgaaatact tgatcacgag gaaggtcccc
    27241 gccagggcga gagtcgccgc gagattggcc gccgccgccc gccggttgtc catctggagg
    27301 aaccggatcg ccagcgccac cgtgagcgaa ctcgtgatga gcacgagggt gttgaccgtg
    27361 ccgcgcgtcg tgttgaggaa ggcgtgcccg ttgtggaata tctccgggtt ccaggcgcgg
    27421 tagacggcgt aggcgcagaa cagcccgccg aagaggagga cctcggtcag caggaagatc
    27481 cacattccga gcttggccga gtctttctgc tgggccgggt cgctgaagtg gtgggccagg
    27541 tgtttcgggt gcccgtggcc ggccgccccc ggcgcttcca tccgttcttt cgccatgctc
    27601 atgagacgac ctccggccgc accgcccggc cgcccggttc cacgttcacc gggatcaccc
    27661 ggtcgtagtc atacggcccg tgggcgagcg acggcgtgaa ggggaaattc tcggtcggcg
    27721 gcggcgatgg cgtctcccac tcgagcgtca gggcgcgcca cggattgctt cccgcccgcc
    27781 ggccccgcgc gagcgagtgg atgaggtaga ttgccataat cacgaaaccc gccccgagaa
    27841 tccacgaccc gaaagtcgag aacgcgtgga gcggctggta ctggtcgagg tagttgtagt
    27901 agcgccgcgg cattccgtgg agaccgagga gaaactggct gccgaaggtg acgttgaaac
    27961 cgacaaagat cagcgcgcac gcgactttcg cccatggctc gttgtacatc ctcccgaaca
    28021 tcttcggcca ccagtggtgc agcccgccga ggaacgcgat caccgtcccg cccatcatca
    28081 cgtagtggaa gtgggcgacg atgaagtagg tgtcgtggac gtggacatcg accgccagcg
    28141 ccccgagcat gatcccggtg aacccgccga tgctgaacag gaacaaaaac gacagcgcgt
    28201 agagcatcgg cgtggtcagc ttgatcgacc cgcggtagag cgtcgtcacc cagttgaaga
    28261 ctttgatccc ggaggggatg ccgacgaaga aggtgaggaa cgagaagagc atggccgcca
    28321 gctccgactg cccgctcacg aacatgtggt ggccccagac gagaaaactc acgaacgcga
    28381 tcccgaggct cgaataggcc actgccttgt agccgaagat cttcttgtgc gcgtgggccg
    28441 tgatcagttc gctcacaatc cccatgccgg ggagaatcat gatgtacacc gccgggtgcg
    28501 agtagaacca gaagaagtgc tggtagagca ccgggtcgcc ccccatggcc ggatcgaaaa
    28561 tcccgatccc cgccacccgc tcgatcacca gcagcagaag cgtgatcccc agcaccggcg
    28621 tcgccagcac ctggatgatc gcggtcgcat acatcgacca cacgaacagc ggcatccggt
    28681 accacgtcat ccccggcaga cggagcttgt gaatggtgac gatgaagttc agccccgtga
    28741 agatcgagga gaagccgagg atgaaggcgc ccagggtcat cgagatcacc gaggtgtcgg
    28801 tctgcgtgct gtagggcgtg tagaacgtcc acccggtgtc ggccgcgccc gcgacgatcg
    28861 agtagagcgc gaagagcgac ccgagcatga agatgtacca gctcgcgagg ttcaaccggg
    28921 gaaacgccac gtcccgcgcg ccgagcatga gcggcagcag gaaattgccg agaatcgccg
    28981 gcaccccggg gatgatgaac aggaaaatca tgatcgcgcc gtgcagggtg aacagcttgt
    29041 tgtaggtgtc ggcgcccacc aggtcccgcc cggggtggag caactcgagg cgtatgagca
    29101 tggccagcac gccgccgacg aagaagaaca ccgcgatcgc ggccatgtag aggatcgcga
    29161 tgcgcttgtg gtcgagcgtc aacagccacg atttccaccc ccgggtggca ttcagataat
    29221 taaccgccgt cgcttccgcc atgtcgttac tcaccggccg cctcgctgtt ggtggtgtcc
    29281 agagacttta tgaaagcaat gagggcgtcg atgtcccggt ccgtcaggat tccctggtag
    29341 gtgggcatga ccggctggaa cccggccgtg actctcgcct gcggctcgag taccgactcc
    29401 cggatatagt tctcgtcgac tgtgacaaag tcgcccgtct gcagcgcctc ccggcggccc
    29461 cacagcccct gccaggtcgg acccacgctc ggcttgccgt ccagcgtgtg gcaggtgttg
    29521 caggcgcggc tcgtgtagag ccggcggccg tagtcggccg gtgactctcc ggccccgggt
    29581 cccccggtcg aggcgaggaa ttccgcgaac tcccgctccg acaccacctt caccttcgcc
    29641 agcatctccg aatgccccgt gccgcagtac tccgtgcaga acaggttgaa cgtcccgggc
    29701 cgcgtcgcct caaaccacgt gaccgagtag cggttcggca gcacgtccat tttcatgcgg
    29761 aaatcgggca cgaaaaggct gtggatgacg tcgcgcgaag acatgagcag cttgaccggc
    29821 cggtcgaccg gggccaccag ttcgttgacg ctgttggcgc cgttcgggta gtcgaacgtc
    29881 cagaaccact tctgcgccgt caccttgacc tccagggcgt cgcgcggcac gatcgtcatg
    29941 cgcatgtagg cgcggaaacc ccatacgaac acgatgagga tgagcacggt cggcaccgcc
    30001 gtccacgtga tctccagcgc gagattgtgg tcccgtccgt aggttgtccc cgtcggccgc
    30061 cgccgccggt agcggatgat gaaaaacacc gtcaggccga tgacgatggc gaagagcacg
    30121 gccgacgccc agaagatgaa gtagaaaagg gaatcgactt caccggccaa agtcgagccg
    30181 gccggcggga aaaacaatgt tccggttgta tccatcgctc cttctcatcc cgccggcgcc
    30241 ggccgatgcc gcgtgcggta cgactcgcgc agccacaaaa ccaagaggaa tccgcccagc
    30301 ccgagcaccg tcagcaggcc gccgaccagc atgacattct gcgccaggac gacatagccg
    30361 ccggcgcccg gatcgtagtg gtagcagtac aaaatgacgc ggtcgagcgt gctgccgatc
    30421 ttcccgtcgg cggcctccac aagcgccagc ttcaggtcgc gcgggttgta ctggatgccg
    30481 tggaggtagc gcgacacgac tccctcgggg gagagcacgt gcaccaccgc cgggtgggca
    30541 tactcgtcgc ggctctcgtc gtagtagtag cggaacccga ccgcgtcggc aagttgcgca
    30601 atcgcggccg aatcgccggt gagaaacgcc cagctcgccg gcggcgccgc cggcccgagc
    30661 tgcgcgaggt agctggcctt cttggccgcc gccaggtccg gggtctcgcc cgggtcgatg
    30721 ctcaccgtga ccacccggta ctgccgcccc aggtccagcc cgacccgcgg cagcgcctcg
    30781 gtcaggccgt tgaacacgag gttgcacagc atcgggcact cgtagtaccc gagcacgagc
    30841 aggaccggcc ggtccccgcc gaaataatcg cccagcgcca cctcccgccc cgcctcgtcg
    30901 accagcccga gcccgcgggg gatggtgtcg ccgaggcgct cgtggacatc gatgccgcgc
    30961 agttcgggcg ggtcggtgcg gaccgtctgg ccggccgccc ccccggccgc cgcgaggagc
    31021 gcgaggaatc ctatatttcg gaggacacgt tgcatggcct gtcttcgtaa ccgccggcgg
    31081 gccggaaagg atccggaaaa ctgtctcctt aaatgatcca tataatccat tttaccggcc
    31141 gcccggagcc gccccccggg gccgtccggc attaccgcgc tttcgcggta aacgcctcct
    31201 gcgcctgaag ttccatggcc cgttcgatgg gaatccggta gctccccgca gccccgtcct
    31261 gcaccccgta cgattccagt tgctcgacct cccgcgcccg cagctcccgc agcgccttcg
    31321 actccggcgc ccgctgctgc tcgtaggaga tttcctcggt cacggcgatg aagtactcgt
    31381 tgagcacgac cgcgaacgcg atgacgagca gggtgagaag caccccgacc acgagcgctt
    31441 tggtcacgtt gacgtcgctc gtgtcgtacc cggccttttc ctcccggccg ggcgccggtt
    31501 tattttcggt gtccgtcata ttccgttcct aattcgtcag ctgcatggac cgctggagcc
    31561 cggggtcgcc caccggcacg agcggccgcc tcaggtgccg ccgccagacg taccagatga
    31621 agatgccgcc gatcccggcc atggtcgccg cgtcgatcca cgaaaacacg gctcccgcgc
    31681 cggggaaatt gggctgcacg atccaatgca gatccaccca gtggatcgcc agcagccaca
    31741 gactcaccgt cgtcagcgcc gccacgttgc gcttggtggc tcggaacacg agcgccacaa
    31801 acggcaggcc gaagtgcccg aacacgatcg ccaggctcac cgctttccac ggctcctgcc
    31861 agcgcacgag aaaccagact gtctcctcag ggacgttcgt gtaccagatg aggaagtact
    31921 gcgagaaggc catgtaggcc cagaatatca cgaacgcgaa catgagcttg ccgagatcgt
    31981 ggtagtgctc cactgtgacc acctcgcgga gcagaccgcg gcggcgctgg tacgacatca
    32041 ggaccaccag cagcccgagg gcgccgagga accccccgga gtagatgtac acgccgaaaa
    32101 tggtcgagta ccagtgcggg tcgagcgaca tgagccagtc gaaggccgcg aacgtcgagg
    32161 tcagcgcaaa cagcagcatc ccgggcgcgc tcaccacccg caggcggcgc cggacgtcct
    32221 ccggtccccc ccggtcctgc agatccgaga tcctgttcag gcggtaggcc aggaggcccc
    32281 acacggcgaa gtagaagacc atccggacaa tgaagaacgg cacgttgagg taggcggcct
    32341 tagcctgcag gaggggatcc tcggccaccg cctcggcgtg ggtccagtgg tagaggtcgt
    32401 gcatcccgaa caaaagcggc accgcgaaca cggccaggag cggtagcccg ctcatgatgt
    32461 tctccgccgc ccggcgcagc acgacgctcc acttcgcgtt gacgaggtgg tggagcatgg
    32521 tgaagaagag cccccccagc ccgatcgtca cccagtacgt gaaagccgtg aggtacgacg
    32581 ggaagaagcg gtgaggatcg acggcgcagg cgacggcgct gagcgcgagg ccggcgacgc
    32641 cgatcgccag cggcgccgcc gcccggcgcg aaccgccgct cacccggtag gtctgcggat
    32701 cgatcgtcat aactgtcccg ctagggccgg agcccccggc ggatttcctc cggtacatcg
    32761 gcggccgagg cgttctggct ccgctggagg gcacgcaggt aggctacgat cgcccaccgg
    32821 tcctcgaccg aaatttggtg ctggtaggcc ggcatattgc gcaggccgtg cgtgatcacg
    32881 tcgaaaatgt ggccgtcggc cacgctccgc agccgcgcgt cgtggaaggt cggcggcggc
    32941 acgtaccccc gctgcaccat gatccccttc ccgtcgccga cccgcccgtg gcagggggag
    33001 cagtagatgt cgaagcgctc gcggccgcgc tgcaagaacg gcaacgtgac cgctaccggc
    33061 agctgccgga cggtgtcccc ggccggggtc acacccgtga agtacaccgc gtcgccccgc
    33121 aattgcccgc gggccaccgt cccggccggc ggcgtccgca taatcgcctc atttccaaaa
    33181 aactccgtcc gctccatcgg ctggtacttg ggctggctgt ccatgtcccg gaccatgtgg
    33241 atgggcgtgc gctgggaggg gcggccgcgg tagcagcccg ccaagagcag gctcgcggcc
    33301 agcagaacga gcatcgccgc ccggccatat gtcaaacgcg cacgcatcat cggcctaccg
    33361 ttcatcctcc ccaccgtcgc cgaccacctc ggctgccgtc gccccgagcg ccaccag
//
LOCUS       DAOPOE010000022        33192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1740_length_33192_cov_6.259136, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000022 DAOPOE010000000
VERSION     DAOPOE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1740_length_33192_cov_6.259136"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PKM94_04845"
     CDS             complement(<1..1723)
                     /locus_tag="PKM94_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66001.1"
                     /translation="MKKQGRLGFVRGILMMSVLLASAMGGSQVVWAQDPPVITCPPGP
                     IDLTLDDIPTAVIELPITGATSVSGGTWANDTIQVTLTMPVKDTIRVIATNTYGADTC
                     DLIVQFPGVTTKKGTMAGAIWTKAESPYCVVGNLAVASLTIQPGVEVLFYGDFELEVT
                     GILTAVGTASDTILFAGAGTQRWQGMLFEATMPGSQLRYCQIVGAQNSGMRLINTEQV
                     IENTEIRGCSSPTGGGAIYLDRRLSVDTLRLRACRFVGNSSPSTHGGGIWAELGTGSL
                     LMQECIVDSNTARMSGGGIISHSTGGHLIVDDCRFEYNGGNPGNSQVGSVYGGAIYAS
                     TASGLIRITRCQIFNNDLYGRVSCTFGCGVNAWGGGAYLSGRTIITNSVFSGNYVYSR
                     VDASTGARNAMSYGGGVCHENGELTIANTVVACNTVYSANGYRAWGAGVYSAGADANI
                     ENSTFANNTENALYRTGDSIAAMNSIFFFNNGTNPEISGNVAVTFSDVQGGYPGTGNI
                     DVGPVFLSPSGCAEEDLTILFPSLCIDAGNPDPAYDDVCFGPSLGGARNDLGAHGGPG
                     ACGWYGFP"
     gene            complement(2052..2474)
                     /locus_tag="PKM94_04850"
     CDS             complement(2052..2474)
                     /locus_tag="PKM94_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66002.1"
                     /translation="MTSYSRTTHAAVLAAVSAAAFFGCSDHRAIQPETPAEIPANSGT
                     IRADLNSVPAGGELLLFCATARMGKAGVVIHWEADAGGFDFADSACVRWTAPGHAAVA
                     HIRVTVSNARGSVTDLVSILVVEPPDPPATRPAVIASL"
     gene            complement(2709..3611)
                     /locus_tag="PKM94_04855"
     CDS             complement(2709..3611)
                     /locus_tag="PKM94_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66003.1"
                     /translation="MNARSLTQITAVVLTATLSLSAAGCGYRIDYRMSEANTPRAADP
                     QPMAVAVALFDDTREIAERSQDGCERAGLQDCGDRTKDKDFRGTVAWEISRVVADHLT
                     WARSFDRVELFPYRTAALTPAVLDSLNGAGIDAVLTGSVDHFVGYYDQHPGTQLLTTA
                     GLAAGLGFAAALLTTETEESTVEMPFQDGFTITETKINPLAISLAAMSGGLLGSLLES
                     TGRRDVDGHALLTLRLLDTKTGQILWAGTAESQDNGHRSMPGFDTAKRKQELALASLR
                     AAVNNLVRQIESFALAEAAAGPSR"
     gene            complement(3677..4138)
                     /locus_tag="PKM94_04860"
     CDS             complement(3677..4138)
                     /locus_tag="PKM94_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66004.1"
                     /translation="MTPTHFPKLAAALTLASLAAYILIPSSASARYHDHSDELPGTDT
                     PVGTYLAVAAGALAVVGIIALVKHSGGKDKDVVPADSSSTGREEPAEETEGEGDAAGQ
                     SSGGESAVSDDAGASRLGLYLGLNPAETIGSDGRRPDFSDLGVKVGFTLSF"
     gene            4256..4822
                     /locus_tag="PKM94_04865"
     CDS             4256..4822
                     /locus_tag="PKM94_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66005.1"
                     /translation="MAGTNQDIDVIDGYLCGDATSCQVIGRYINAALGPFRERLGYQV
                     DDVRSDVEDELRTALGERQFRGEAQLKTFVSRVVSHTAIDYIRYNRRFADGDPEEAIR
                     LLPDKLPDPERELERNQLLRLLWRVVRRLPRDCVKILRMHERDGLTCAMIGQVIGKKE
                     DRVRRRMWECRREAERIREELLRPDKRL"
     gene            4869..5696
                     /locus_tag="PKM94_04870"
     CDS             4869..5696
                     /locus_tag="PKM94_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD66006.1"
                     /translation="MDDLGNYDDRIGRYIHGRLTEAERVEFEEALGRDPSLVEEVGRA
                     RLLAAGLAVQAHVTGDHPEPLDITAFALEPSELDSGLRSRIEAHLRDCPECRVEVELC
                     RQSAQAMVDRPVPASAIRPRWWAWLAGAQPRLAVATVAAAALVLFTGLFLGRTFFASA
                     PPTARIELSAASERGEQTKNLVRIGAGSELVSLEFLVPSREGRRYDLVLADGAQNEIL
                     TLHNQLHRIPFSLAVPAAYLAAGSYEILVIEQVDASQPMDTLAPDTFRIRFETAPAR"
     gene            5719..8511
                     /locus_tag="PKM94_04875"
     CDS             5719..8511
                     /locus_tag="PKM94_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HOD66007.1"
                     /translation="MACACLILFTAAVLTSRNADDQPAAPEPPRLAAIAAARDSAAFD
                     SALAVDAAGVRRYLKTAVTDLTRRAAEGADAAGGGESLGVNWLVDRYTGVTSVGDLSR
                     QRDLLYAWGPAQAAAKTTCDSLFRRGNRVFQRERDDGRAGAGARFLSLLDTLAARYAA
                     LGDGPGRAAVWSYRAGILEQGGDLAVAIAAADSARALAEAFDAPDLLGDVHLRRSEIA
                     LVRQADYLAAGLHHRQAVEAFRRIGDQRRILTAALQQFYALHQLNRTEEALRAVREAM
                     QSARHLNDRRSESYCAHVISECLFDLGDFDSALTYNDRCTSLRLPVDIALPRPSALSD
                     LGYALATRGLVLHALGRTSDARYFYEWADSLFSKAVDTLGLFLSGGRLGNLLLDEGRW
                     EDAEGVFLPILARAGKFETRLAAAYGLGVAEYRRGRPQEARIYLRECVRQCEQMRGRL
                     PSPDLQLGMLADKAGFYDLLVVTFLEEYMAGGSSASLDSALAYLDLAQARSLVESAGE
                     AVGSPCPEENRLLSRLSDLQFSLLVDRDDPAGLGRSQRALEDSLFAARVECAAVSPSD
                     SHPPALPTSRTLCSTVLPRDLVLCWVLSPAGTFAIALSADTVVVRPLAASRDEIAELV
                     REFVAAVAAYPTARDFPESPYHAPGRKLYDILLKPFVNEQDTTRLTVIAPGALAGLPV
                     GALVDAHGRFVVESREVVYAPALSMLARHPEEAERSDPRRMLAFGDARYDDNSVLSGL
                     PFSSEELDRISRVYGANASVYADSAATEANVRRTADSWTGILHLAVHGLVDPDDWSRS
                     ALSFSFDSDGRGSGLLHASEIAHLNIPAALVFLSACESGSGRQIAGEGVMSLARSFLA
                     AGAGSVISTLWRVDDRASAEFVGQFYDRTAAAAPPAEAIAEAQRLMIHSDRPLYRHPY
                     FWASYVLTQTGARKTK"
     gene            8932..9075
                     /locus_tag="PKM94_04880"
     CDS             8932..9075
                     /locus_tag="PKM94_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66008.1"
                     /translation="MRSALSLDGIECYPNVEVTPSVTVSLMAEIDRVIEARGGLPGAF
                     KAD"
     gene            9200..11584
                     /locus_tag="PKM94_04885"
     CDS             9200..11584
                     /locus_tag="PKM94_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOD66009.1"
                     /translation="MSEHFLSALRAQLRAHGQAPLRVIGIDLGTTNCTVAEIVADGAA
                     EDFEPRCIEIEQETPFQGEYTHILIPSIVALHGGREYVGEGAKRLRAGLAGPETGLER
                     NRTIFWEVKNEMGIQRTYARAPEGYRGAAEVSGRILRFLANRYRADHGEPRKAVVTVP
                     ASFQQPQREDTLRAAKLASMELAGGDLLDEPLAAFVDFWMRGELAKALPRGEGNVLVF
                     DFGGGTCDTAVVKVHAGDAAGHLRASPLSVSRYYRLGGGDIDLAIFYEVLLPQIMEQN
                     GLSERDLDFGVKKNQLEPAFLGIAESLKVGLCTQVRRLRELARGAELPIEPVQKLPAT
                     ERCRLTNGREITLQSPALTLPQFEAVLEPFLDRTFVFHRETEYRLTCSIFAPLEDALD
                     RAELDAREIDCCLLVGGSSLIPQVADALRSYFEPEKVVLYSHPDQVQSAVARGAAAQA
                     LSLAVLGRGVLQPASPTGVCIRTTAGKMQLIPPHSPLPYPAAGEWAMNTNLALPKRLS
                     QTERVVRIELIGTDDSLIQRWTWLVPPAAAPGEPLTLQYCMDENQVIRYRLDLESGTG
                     REGLTGSTENPIHNVVNPDLKRIRILELEECLRTSLPAGEQQEEMVVEIANLSRELRQ
                     YDKARYLLERVLRTRGGGDIGVLNYLGIVCGEQGDRQAEEKYYREAARLGSDAAQFNL
                     AYFYRDHGSLAKAAEAIERLVERFPKGPYLVLKADIAEREERIYESKRALEMARKAFG
                     PVKSLSDWELSWYEHGAKLAGDEAAQEMVRAERKRRRRSNAPEPSGLLPEMREA"
     gene            11593..12852
                     /locus_tag="PKM94_04890"
     CDS             11593..12852
                     /locus_tag="PKM94_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD66010.1"
                     /translation="MGVWLVPESELTPDQIRAVQLPTDGHKVVSGPPGSGKTLVLVHR
                     ARYLLDSKGITGGQLRLFVYTRTLRDYIQSALKLLGIADECVLTFDAWCRSFYLSCVG
                     NPPPRDEVNGQLDFREIRRAVLRALETAPVAPVYDYVLVDEGHDLDEISYKILVRIAR
                     HITVCMDRKQQIYDDGAAEERVLHILGLPKRSVALLEAYRCCPYITRLAATLLDRQER
                     QPYLGQMRTEQVEREKPLLFIANAQEAELHELARQIRERQRVQGRNDGGGGSIAILFP
                     QKRMVFGYANALADLGVQVEVHLDRPAQQARYEMVDFRNNLPKILTYHQAKGLTFDSV
                     FMPRLVGYAFKRVSEPRLRRLLFVGITRAKSWVYMSTEDSRMLPCLNAICDESGDFLA
                     VRYSGGNDGPKPDTDSGEEDGLANLFT"
     gene            14130..14324
                     /locus_tag="PKM94_04895"
     CDS             14130..14324
                     /locus_tag="PKM94_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66011.1"
                     /translation="MLVPALSAFVVPLPATAEGPFDPHIDSRPGCHGCFTETGCIFFA
                     FFCSGLVMIIDWAHVSLMAY"
     gene            14341..15189
                     /locus_tag="PKM94_04900"
     CDS             14341..15189
                     /locus_tag="PKM94_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66012.1"
                     /translation="MERGSMNYWLIGYEQKRSYQGNLLRALLSTALLFIGLGVLIRFA
                     EPGAERRVSWSDRLLGGVAAETAAIQPTGRGGGAGGGQGASHAAPFLRGYVGPVVIIR
                     DAPSTIRPAVAVLPGPSEPDPLPSPVPGEPGWGGDFAGQGDNPFGLPASEPYLPPVGN
                     GFDGFDGLGGDGLNWAPAIRIRPAGWPMDADNVPRVDSGVVWLTLTIHADGRLSFVVD
                     SAYPEARGFVPNVEWALIQSRVEPALQAGEPVATTFAMTYIVRRGGVESVVHSKTGYV
                     RGFVAE"
     gene            complement(15398..16471)
                     /locus_tag="PKM94_04905"
     CDS             complement(15398..16471)
                     /locus_tag="PKM94_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66013.1"
                     /translation="MKRVLLFVVLAALSAAGCSRDGQRPSAPDQSAMRPGEMPGAVAA
                     VLDRHAADQGDVASAALRYPEFTGPGALPWTAWDAYAVTFVWGEFPLGPAAVVPPTDW
                     SGSLAIENAAGLLDVVAVIDFEPGQDSVLPSASSPTALWTSFTEGDLDGLSFLVFLDP
                     ESDAAANARVRFKTPLCELALTRDQLTRFTAFYIVGHTHVVAVKARRIWHHACPRGFI
                     SGEWTLQSNTGDSGYFSGLWYDRTNTPIGVMSAVYRKTPEGDRVYAGSISGVLLDVVI
                     GEVSGIWQYDDPRMCPTCGAGHGWFRGRFVFYEMGGEPRREGCMAGIFGNLSAPTPPA
                     LPLRGIWIVDCPDSTSVVSPTAD"
     gene            17216..17836
                     /locus_tag="PKM94_04910"
     CDS             17216..17836
                     /locus_tag="PKM94_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD66014.1"
                     /translation="MEKWQGWDKIRYESELDHHFADEIASIPGGDKLFRCIQCGTCSG
                     MCPLSSYMDYTPRQIVAMIRAGFRGEVLSSYTTWLCASCYACTVECPQEIKITEIMYA
                     AKRLAIRGGMHPKRFPSQVLATEFFRGVERKGRSNEGPLLVRLFLRTNPFKMFGNTGL
                     GLGLFRRGRLSPKLESIKRTGELHTMLAAIEQERMVRRTETTEATA"
     gene            17833..18732
                     /locus_tag="PKM94_04915"
     CDS             17833..18732
                     /locus_tag="PKM94_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOD66015.1"
                     /translation="MKYLYYPGCSLKSTGKSYEESILAVFSALGRPLEELDDWNCCGA
                     TAYMSISEMKAFALSARNFAIAERQGDSDTHLVVPCAACYLGLNKAHRYLDEHPDIRK
                     TIQSALAAAGLSYENGIKVRHPLDVLANDVGLEAIKGAVTRPLAGLKVACYYGCQLVR
                     PYADFDDAHEPMVMDRLITALGGTPVAWPLKTRCCGGSLTGTVPEVGLRLSYVLLREA
                     QKRGCDVIATACPLCQFNLECYQPKMSRMYESRVNVPVVYFSQLMGTAFGLDRRPLGL
                     QRLFVPFAYREPAAAVEGGAHVG"
     gene            18722..20722
                     /locus_tag="PKM94_04920"
     CDS             18722..20722
                     /locus_tag="PKM94_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOD66016.1"
                     /translation="MSADQSSNGTPKVGVYVCHCGINIAHTVDVKACTDFAASLPYVT
                     VAKEYKFMCSDPGQEIIQQDIREGKVNRVVVASCSPLMHENTFRKATAVGGGNAFMFQ
                     MANIREHVSWVTLDTAMATEKAKALIAGAVTRIVRHVPLQRNRVPVHPDVMIVGGGIA
                     GIHAALTMADAGKKVYLVEREPTIGGHMAKFDKTFPTLDCSACILTPKMSAVRSHPNI
                     TLWTYAEVAGVEGFVGNFKVKVRRKPRYVIEEQCVGCLDCIDACIYKTAKVPDEFNVG
                     LSKRKPVYIPFPQATPLVAVIDPETCIEFKTHRCKKSCLEVCQKNAIDFSQTEKIEEV
                     PVGAIILATGFKTFDARRIARYGYGKYPNVYSSLEIERMVNAAGPTGGEVTMPDGAKP
                     KAVGIVHCVGSRDAHTNRYCSRVCCMYSLKLAHLIKERTGAEVYNFYIDMRTPGKGYE
                     EFYDRLLQENVHFIRGRVAEISDWAMSAEEEGKLTIRVEDTLVGAVRRIPVDMVVLSV
                     GLEPQADADEVRRQFNISCSTEGWFLERHPKLAPVSTFTDGIFLAGACQGPKDIPDCV
                     AQAGAAAAEALALIDRGHVELEPNTAYSQEELCSGCHTCIALCPYSAIVFDKAKNAAI
                     VNGALCKGCGTCVAACPSGALQQNLFTDEQIFDEIKGVLSYV"
     gene            20715..21230
                     /locus_tag="PKM94_04925"
     CDS             20715..21230
                     /locus_tag="PKM94_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD66017.1"
                     /translation="MSKTESSRKEFEPRIVAFFCNWCTYTAADLAGTARMKYAGNVRV
                     IRTMCSGRLDPQFVFAALRQGADAVLIGGCHPGDCHYQEGNYKALRRVVLMKQFLAAM
                     GIEPERVRLEWIAASEGDKVQRVINEMTEAVRALGPLRLEQRTLLHAAELPAGQAGRT
                     AEPAGAEGGRR"
     gene            21227..22213
                     /locus_tag="PKM94_04930"
     CDS             21227..22213
                     /locus_tag="PKM94_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOD66018.1"
                     /translation="MSKPKVAFYWCASCGGCEEAVVDLDEAILKVVEAVDIVFWPVAL
                     DFKRTDVEALPDGSIAVSFINGAVRSSEQFEMVEMLRRKSQLVVAFGSCAHLGGVPGL
                     GNLYRREELTATAYREVPSVNGGNGVEPAVSSTVPEGTLTLPELWKTVKTLDQTIAVD
                     YFLPGCPPPVKLILGAVEAILAGKLPAKGSTLAPDMALCDECPRKETKPEKLTVKEFH
                     RPHEILIDPEQCLLAQGLLCLGPVTRQGCGGACTKVSMPCTGCLGPTSRITDFGAKAM
                     SAMASIIEAKDDAGIEATLARIVDPAGTFFRYSLPASLLHRKYEPETTRGMK"
     gene            22210..23679
                     /locus_tag="PKM94_04935"
     CDS             22210..23679
                     /locus_tag="PKM94_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOD66019.1"
                     /translation="MTTTRRVTIDPITRLEGHGKIDILLNDRGDVDHAYFQVPELRGF
                     ERFAHGRPAEDMPQITSRICGVCPTAHHMASTRTLDDLYKVEPPPAAHKVRELLYNIF
                     FVEDHALHFFYLAGPDFVVGPTAPKAERNIIGLLGKVGIEAGKKVIGMRRDLRELMVL
                     AMGKAAHPAFGLPGGISRPIPPEHQKAFIEVCERAVEFAQFSLKVFDDVVLANKTYVD
                     YITSDTYTHRTHYMGLVDAHNRPNYYRGELRVVGPDGREVCKFPVAKYLDHIAEHVEP
                     WSFMKFSYLKDVGWKGFVDGPDSGIIAVAPLARLNAAEGMPTPLAQKAFERFYQTFGG
                     RPVHHTLANHWARLIELLCAAERARELSRDPEIVSKDVRVLPTKTPSEGIGVVEAPRG
                     TLIHHYRTDERGVIKMANLIVATQNNAARISLSVDKAARGVISGGRIDDGVLNLVEMA
                     FRAYDPCNGCATHTLPGSMPLVVRVYDAAHNQVQEIRRD"
     gene            23679..24194
                     /locus_tag="PKM94_04940"
     CDS             23679..24194
                     /locus_tag="PKM94_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOD66020.1"
                     /translation="MMKPVVMGMGNELFGDDGFGIIAARRLAPLAGEAADVIECGAAG
                     LALLDPLIGRPRAVIIDAICTGRQAPGTIYRVSPADLRDITNLSPHYAGLPEVIRMAQ
                     RLELPIPDTIDIIAIEAADITTMGAGLSPAVAAALEPVVALAVELLLGGRRTGSPAAD
                     AQGISAAGTHV"
     gene            24547..25542
                     /locus_tag="PKM94_04945"
     CDS             24547..25542
                     /locus_tag="PKM94_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66021.1"
                     /translation="MRHHAVLQGTYAQTTLLRSALLKAARAWFEREHFLEICVPHITG
                     ATGSCEWFPNAMPVTMYDPAGAETPMFLRQTGQLYLEAFTQAHNRVYTIGPSFRQERK
                     VTDRHLCEFTLIEFEGRDFELDGLMNHIEGLVKTMYRAARRVMPTDRLTRYIRSPFNR
                     ITYREAVRLLCARGHGLTYGDDLGSAEEKALCAHLGGLPTFVTHYPADPRPRPGGVIK
                     FFSMKRREGETLCCDLLLPFVGESVGGAVREGCAETCRRQFRESYMYDHIVGAGIDPR
                     QFDWYFEVLSHGTGQSAGCGIGFERVVQSILATRQQEVTIKGAVELPRSPDFLVP"
     gene            complement(25583..26812)
                     /locus_tag="PKM94_04950"
     CDS             complement(25583..26812)
                     /locus_tag="PKM94_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HOD66022.1"
                     /translation="MNRRLVSAAWAVAALVAGAAAPAEATFSIVAVDTVNGVIGGAGA
                     SCIDNCFIIDDIIESIGAVHTQAYWLQGNKNYAHQLLAQGLTPDSIILLLVQNDVEGT
                     PEYRQYGVVTLAGSGASAAYTGSATSDWAGHLTGPGYAVQGNILLGPEVIADMETAFL
                     GTDGPLEAKLMAAMEAAKRPGADNRCIFAGKSAISAFIKVVRPGDGGTPFLQEIVPTT
                     TGSTDPIDVLHAQFDTWRLRQAAHPDSSTVVIGKTALDGDGQASTVITIAPRNWAGAP
                     PVRIDSVRVGTTGQAVLSAVADNGDGTFACVLTAPLYGAPDDTLTVTVGAYYDFVTEL
                     HSRPAVTYYYCGDVTGDGSIKVSDLTFFVNFKFRGGPGAAFPSSADMNGDGQVKISDV
                     TYIVAYLFAGGPPPICE"
     gene            26953..27909
                     /locus_tag="PKM94_04955"
     CDS             26953..27909
                     /locus_tag="PKM94_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD66023.1"
                     /translation="MERREFLKRTGRAMVLAAASGGIGVLFHNRNAAGYRPLAVFDRR
                     FTVFGDPACPAVAFARHPDPEHALDAALAAVGGIGRFVKPGETVTLKPNVGWDRTPEQ
                     AANTSPALVAAMVRRCLGAGAAAVVVTDMSCNDPRRCFLRSGVRDAAAQAGARVTIPA
                     AGDFVEVDLDGELLTAWPVLRALLHTDRLINMPIVKHHSLTACTIGLKNLYGILGGMR
                     HRLHQQIDQSLVDLTRFCTPTLTVIDATRVLLRNGPQGGSTDDVAVENAVLCATDPVA
                     GDARGCELLGLTADRVPHLLRAEAAGLGRIDYRNAGYRDVTA"
     gene            27906..29600
                     /locus_tag="PKM94_04960"
     CDS             27906..29600
                     /locus_tag="PKM94_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD66024.1"
                     /translation="MTIAAVRHLRRASQALFFAAFFWLILKTTFEADFRATGGGEIRL
                     PYPVSIGLELDPLVGLANLLANGTVYSGLLLGLVVLLPTIFFGRFFCGWVCPLGSLNH
                     WLSEVRSERAANRGARKIEANRYRGYQRIKYYLLLVFLGAALAGVLQIGLLDPIALLA
                     RSLGTVVLPALHVAAEGVRDAVAAVGFAPLTNAAQRVYDTLGPLVLPFRPQFFHALLT
                     IGALFVAILWLNRLFTRFWCRGICPLGALLGLFSRLAVFGLEKNAASCDHCNKCLLHC
                     QGADNPDVGSAWRQEECVLCLNCQAACPHGSLRFRFFPRLEGTRDNPAPVRTPHLTRR
                     KVLGSLAGGLALFPLFRAGDAFSANPNPLLIRPPGAPPEDDFLARCIRCGQCMRVCPN
                     NALHPTLLESGFEGLWTPVLIPRIGYCEPSCTLCGQVCPTGAIEELTLSEKVGDRATP
                     GVHIGTAFVDRGRCLPWAMAIPCIVCEEWCPVSPKAIYLREEIAYDRNGDAVNVKRPF
                     VDPAICNGCGACEFACPVADQAAIRVSSVGESRSRENRILLDRRPARREPSGPRAN"
     gene            complement(29649..30302)
                     /locus_tag="PKM94_04965"
     CDS             complement(29649..30302)
                     /locus_tag="PKM94_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HOD66025.1"
                     /translation="MRGERLRRAAAGAPPAGEAGFDLSRELSFHPDTGRTLFRDSRVL
                     ILDANAIGMLRQNLLDTLGWAQARDFLLRFGYQHGYSDFMQIRHNFEFADEMDLLCAG
                     PVLHTYEGIVKAAPTEIRFDRHSREFQFAGIWTNSWEAEQHLAFNPPSPQSVCWTLTG
                     YATGWCSAFCGWPVLAMEPTCIGKGDPHCGWKVQPPDLWGEEGRPMIEALSVFWASR"
     gene            30491..31609
                     /locus_tag="PKM94_04970"
     CDS             30491..31609
                     /locus_tag="PKM94_04970"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOD66026.1"
                     /translation="MNNSDQLFTADLHCDTIHRILQGADFSRRNEIGHLDLPRLIEGG
                     IDLQVFACFTESFIEAKDRVPLVHRMLDGLHRTVEAHGEKVALARTSSEAARNRAAGK
                     ISAVFSIENGLVLNDNLDTLRQFYERGVRSLTLTHNVSSAWCISSADEHPAFDGLTAF
                     GREVVRMMDELGMIVDLSHAAPSAVAAVLEIATRPVIASHSCVRALCDHHRNLTDEQI
                     RGIAATGGMVGINFLCDFLSPACRDASAAYINAHYDLGRRYWLLFTAECGAEEYAARR
                     AELQPFLAGWEQVVRATGVDVGVVADHIDYIVRLVGPDHVGLGSDFDGITFAPAGLED
                     CAKMPRLADELRGRGYCREDLAKIMGGNFLRVFREVCG"
     gene            31832..>33192
                     /locus_tag="PKM94_04975"
     CDS             31832..>33192
                     /locus_tag="PKM94_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOD66027.1"
                     /translation="MSLHRSARRAALPLFFGLLLAAPYVSAQISEGGTPPSRMVRAGA
                     LAAATSLPTVDTGPVDVPALLAEDAAEQDKGVPFRFGYPFETVCDLTNSGQWDTLSDG
                     GRLWRLRIVAPGAYSINLVYRKFWLPPGAKFFVYSEDYRYVIGAFTERNNKEYGEFAT
                     QPVPGSVSILEYYEPPNAPEPGIISLQRIVHAYRDIFATATMKEAAGFGGSGSCNNNV
                     NCPEGDLWQDDKRAVAMILTSGGYRICTGALVNNVREDLTPYFLTANHCLGGESTWIF
                     MFNYESPSCANIDGPTWMTVQGSVRRANYSSSDFALLELLEQPPDSYNVYYAGWSAVD
                     VAAQSAVGIHHPSGDIKKISFDYDAVVSANYAGSTGGTHWRILQWDDGTTEPGSSGSP
                     LFDSLTHRIVGQLHGGTASCASLTSDYYGKVARSWTGGGTSTSRLSDWLDPDNTGTLA
                     VDGL"
BASE COUNT         5652 a        10888 c        10870 g         5782 t
ORIGIN      
        1 atgggaaccc ataccaaccg cacgcacccg ggccgccgtg ggcaccaaga tcattgcgag
       61 caccaccgag tgatggcccg aagcacacgt catcataggc gggatcaggg ttcccggcat
      121 cgatacagag agaggggaat agaatggtca gatcctcctc ggcgcagccc gacgggctta
      181 ggaacaccgg tcccacatca atgtttcccg ttccgggata accgccttga acatcgctaa
      241 aagtaacggc cacgttgccg gatatttcag ggttagtacc gttattgaag aagaagatcg
      301 agttcatggc ggctatggag tcgccggtgc ggtacaacgc attctcagta ttattggcga
      361 atgtgctatt ctcgatatta gcgtcggccc ctgcagaata tacacctgca ccccacgcgc
      421 gatacccgtt ggcgctgtat acagtattgc acgcaaccac cgtattggcg attgtcagtt
      481 ccccgttctc atgacagact ccgccgccgt agctcattgc gtttctggca cccgtagacg
      541 cgtcgacgcg gctatagaca tagttgccgg aaaataccga gttggtaatg attgttctcc
      601 cacttaagta cgccccccca ccccaagcat tcacgccgca tccaaacgta cagctgacgc
      661 gtccgtacaa gtcattgttg aatatctggc atcgcgtgat ccggatgagt ccacttgcag
      721 tagaggcata gatcgcaccg ccatagacac ttcccacctg gctgttgcct gggttgcccc
      781 cgttgtattc gaatctgcag tcatctacga ttagatgccc gcccgtggag tggcttatga
      841 taccgccgcc gctcatccta gccgtgttcg agtccacaat acattcctgc atgagcaggc
      901 tgcctgtccc caattccgcc caaatcccgc cgccatgggt agacggcgag ctgtttccca
      961 caaaccgaca agccctcagg cgcaacgtgt cgacgctcag ccggcgatcc aggtatatgg
     1021 caccaccgcc ggtcggcgac gagcaccctc gtatctctgt gttctcgatt acttgctccg
     1081 tgttgatcag gcgcatgccg ctgttctggg cacccacgat ctggcaatac ctcagctgcg
     1141 aaccgggcat ggtcgcctcg aatagcatcc cttgccatct ctgtgtgccc gcgccggcga
     1201 agagaatggt gtcactggct gtgccaacgg cggtgagaat gccggtgact tccagttcga
     1261 agtcgccata gaacaggacc tcgacaccag gctgaatcgt gaggctggcc accgccagat
     1321 ttcccacaac acagtagggc gactccgcct tggtccatat ggcacccgcc atcgtcccct
     1381 tcttcgtcgt cactcctggg aactgcacga tcaggtcgca tgtatccgcc ccatatgtgt
     1441 tggttgcgat cacgcggatg gtgtctttga ccggcatggt cagagtcacc tggatcgtgt
     1501 cgttcgccca tgttccaccg gagaccgagg tagctcccgt aatgggcaac tcaatcaccg
     1561 cggttgggat gtcgtcaagc gtcagatcga taggccctgg cgggcaggtg atgaccggcg
     1621 gatcctgcgc ccagacgact tgagatccgc ccatcgccga tgccagcagc acgctcatca
     1681 tcaggatgcc ccggacaaag ccgaggcggc cctgcttctt catacactcc tccttggacc
     1741 gaaacagccg aggctgttca tgatatgatg tggaatcgct atccccacgt tgagaacgag
     1801 gaacctgacg ttcgctaacg caaattaccg atctgagatc cattgtcaac acctccttgc
     1861 cggaaaatga tttcttgctc gggatttcgt tccacccgca accgatcgtg gcggcgtcgc
     1921 accgtgcgaa gggattctgt cgaaatccgg accattgtgg ttctccaatt ctgtttaccg
     1981 gcctgtggtt gataggcgaa ccacattggc ggtcgtttgt cacggccgac cggaaaagaa
     2041 ccatttgcgg gctacagact ggcgatcacg gccggccgag tcgccggcgg atccggcggc
     2101 tcgaccacga gaatcgagac tagatctgtc acggatcccc tggcattgga cacggtgacc
     2161 ctgatgtgcg cgacggcggc atgccctgga gcggtccagc gcacgcacgc gctgtcggcg
     2221 aaatcaaagc cgccggcatc ggcttcccag tggatgacaa ctccggcctt ccccattcgc
     2281 gcggtcgcac agaacagcag gagttctccg ccggccggaa ccgaattgag gtcggctcgg
     2341 atggtcccgc tgttggccgg gatctcggcc ggcgtctccg gctggatagc ccggtgatcg
     2401 ctgcaaccga agaatgccgc cgcgctgaca gcagccagaa cggcagcgtg cgttgttcgc
     2461 gaatagctgg tcatgggatc ctccttgtct ctgcgggtgg gtagtcaccg gctttgatcc
     2521 tacaggcgaa cggcggggga aagggtttgc gggagaatgt cggtcgcgcg ggatcagttt
     2581 ccgtttgggc ggttgcgcgc gggagcagag agcgacatcg cccaatagaa cgccccggag
     2641 accttttcgg tcctcggggc gttgcgagac cctcccctga caggcggcat ggcgacccct
     2701 gttttggtct agcggctcgg accggccgct gcctcggcca gcgcgaaact ttcgatctgc
     2761 cgcaccaggt tgttgacggc cgcccgcagc gatgccaagg caagttcctg cttgcgcttg
     2821 gccgtgtcga atccgggcat ggagcgatgt ccattgtcct gcgactccgc tgtgccggcc
     2881 cacagaatct gccccgtctt cgtgtccagc aaacgcaggg tgagcaatgc atgaccatcg
     2941 acatcccgcc tgccggtgct ttcaagcagc gatcccagaa gccccccgga cattgccgcc
     3001 aaactgatcg cgagcgggtt gatcttggtt tccgtgatcg tgaagccgtc ctggaacggc
     3061 atctcgaccg tggattcctc ggtctcggtg gtcaggagtg ccgctgcgaa ccccaaacct
     3121 gcggccagcc cggcagtcgt cagtaattgt gtgcccggat gttgatcgta gtacccgaca
     3181 aagtggtcca ctgatccggt gagaacggca tcgatccccg cgccattcag cgaatccaga
     3241 accgccgggg tgagagcggc tgtccggtag ggaaagagct ccactctgtc gaatgaccgg
     3301 gcccaggtca ggtgatcagc caccacccgg ctgatctccc acgccacagt gccccggaag
     3361 tctttgtcct tcgtccggtc gccgcagtct tgaagccccg cacgctcgca tccatcctgg
     3421 ctccgctcag caatctcccg ggtatcatcg aagagcgcga ccgcgacggc catcggttgc
     3481 gggtcggccg ccctgggtgt gtttgcttcc gacatgcggt agtcaattcg gtagccgcac
     3541 ccggcagcgg agagcgacag cgtcgcagtc aagaccaccg ccgtgatctg agtcaggctt
     3601 cttgcgttca tgagagttca ctttcctcca caaatgatcg ccccgggcct ccgcccgggg
     3661 cgtgcggttc gtgcgttcag aagctcaggg taaacccgac tttcacgccc aggtccgaga
     3721 aatccggcct gcggccgtcg gacccgatcg tctccgccgg gttcagtccc aggtagagcc
     3781 cgagtcgcga cgctccggca tcgtccgaca cggcgctttc gcctccggac gattgcccgg
     3841 cggcgtcgcc ctctccctcc gtctcttctg cgggctcttc ccggccggtc gagctgctgt
     3901 cggcgggaac cacgtctttg tccttgccgc cgctgtgttt gacgagagcg atgattccga
     3961 ccaccgccag cgcccccgcc gcgacggcga ggtaggtgcc gaccggtgta tcggtgccgg
     4021 ggagctcatc gctgtgatcg tggtatctgg cggaagcgct tgacggaatc aggatgtacg
     4081 ccgcaagaga tgccagcgtc agcgccgccg cgagtttcgg gaagtgggtg ggtgtcatgt
     4141 ctgtgtacct ccgttggaaa gggtcccttg atcggcaatc cgtcatttct tctcctccgg
     4201 cgagcggccc gacaaaacgt ttgcggaaga atcgactaac gtgtagattt ccgccgtggc
     4261 agggacaaac caggacatcg acgtgatcga cggctatctc tgcggggatg ccacgagctg
     4321 ccaagtcatc ggacgctaca ttaatgccgc gctgggtccg ttccgggagc ggctcgggta
     4381 tcaggttgac gacgttcgct ccgatgtaga agatgaactc cgtacggcgc tgggggagcg
     4441 acagttccgc ggcgaggcgc aactcaagac attcgtgagc cgcgtggtca gccacaccgc
     4501 gatcgactat atccgctaca atcgccgctt tgccgatggc gacccggagg aggcgatcag
     4561 actcctgccc gacaaacttc ccgatcccga acgggagctg gagcgcaacc agttgctgcg
     4621 cctgctgtgg cgggtggtgc ggcggctgcc ccgggattgc gtgaagatcc ttcggatgca
     4681 cgaacgggat ggtctgacgt gcgcaatgat cggccaagtc attgggaaaa aagaggatcg
     4741 ggtccggcgg cgcatgtggg agtgtcgccg cgaggccgag cgaatccgag aggaattgct
     4801 gcgacccgac aaacgtttgt gagcctgctt cgccggtagt tcgagcgtta tggcgaagga
     4861 gaatatacat ggacgacctt ggtaactacg acgaccggat tggcagatac attcacggcc
     4921 gcctgaccga agcggagcgg gtggagttcg aggaagctct gggccgggat ccgtccctgg
     4981 tggaggaagt cggccgggcg cgcctgctcg cggcgggact ggcggttcag gcgcacgtca
     5041 ccggcgacca cccggaaccg ttagacatta cggcctttgc cctggagcca tcggagttgg
     5101 acagcggtct ccgcagccgc atcgaggcgc acctgcggga ctgtccggag tgccgcgtgg
     5161 aagtggagct gtgtcggcaa agcgcccagg cgatggtcga ccgccctgtg cccgcatcgg
     5221 caatccggcc gaggtggtgg gcgtggctgg ccggcgccca gccgcgactc gctgtcgcca
     5281 ccgtggccgc ggcggcgctg gtgttgttca ccgggctctt tctcggcagg acgttcttcg
     5341 cgtcagcccc gcccacggcc cggatcgagc tctccgcggc gtccgagcgc ggcgagcaga
     5401 ctaaaaacct cgtcaggatc ggcgccggca gcgaactggt ttcgcttgag tttctggtgc
     5461 cgtcgcgcga gggccgccgt tatgacctcg tattggccga tggcgcacag aatgagattc
     5521 tcaccctgca caaccagctc caccgtattc cgttctcgct ggccgttccg gccgcgtatc
     5581 tggccgccgg atcctatgaa atactggtta ttgaacaggt cgatgcatca caacccatgg
     5641 acacgcttgc acccgatacc ttccgcatcc gtttcgaaac cgcgccggct cgatgaggga
     5701 accgtaagcc gagcggtcgt ggcttgcgcg tgtctgatcc tcttcaccgc cgcggttctt
     5761 acctcgcgga atgcggacga ccagccggcc gcgcccgagc cgccgagact ggcggcaatt
     5821 gccgcggccc gggacagcgc cgctttcgac agcgcactcg ccgttgatgc cgccggtgtc
     5881 cgacgatacc tgaagacggc tgttacagac ctcacgcggc gcgctgccga gggagccgat
     5941 gccgccggcg gcggcgagag tctgggtgtc aactggctgg tggatcgcta cacgggagtc
     6001 acttctgtcg gcgacctgtc tcgacaacgg gacctgctgt atgcctgggg tccggctcag
     6061 gcagccgcga agaccacgtg cgattctctc ttccgccggg gcaaccgggt cttccagcgc
     6121 gagcgcgatg acggccgggc gggagcgggg gcgcggtttc tgtccctgct cgacacgctg
     6181 gcggcccgat acgcggctct cggcgatggt cccgggcggg cagccgtgtg gtcgtatcga
     6241 gccggcatcc ttgagcaggg cggcgacctt gccgtcgcga tcgcagcggc cgacagcgcc
     6301 cgcgcgctgg ccgaagcctt tgacgcaccc gatctcctgg gcgacgtcca tttgcgccgg
     6361 agcgaaatcg ctctcgtgcg gcaggccgac tacctcgccg ccggcctgca ccaccggcag
     6421 gctgtcgagg cctttcggcg catcggcgat caacgccgga ttctgaccgc cgcgctgcag
     6481 cagttctatg ccctccacca gctgaatcgg accgaggagg ccctgagagc cgttcgcgag
     6541 gccatgcagt cggcccggca tctcaacgac cggcggagcg aatcatactg cgcccacgtc
     6601 atcagcgagt gtctgttcga ccttggcgac ttcgactcgg cacttaccta caacgaccgc
     6661 tgcacatcgc tgcggctccc cgtcgatatc gctctccccc gcccaagcgc tctgagcgac
     6721 ctcggctacg cgctcgccac ccgggggctg gttctgcatg cgcttggacg gacctccgac
     6781 gcccggtatt tctacgagtg ggccgattcg ctcttctcga aggccgtcga taccctgggg
     6841 ctgttcctca gcggcggccg gctggggaac ctgttgctcg acgaaggccg ctgggaggac
     6901 gccgaaggcg tgttccttcc catcctggcc agggccggga agttcgagac gcggctggcg
     6961 gccgcttacg gcctgggagt cgcagagtac cggcgaggac gaccccagga agcacgcatc
     7021 tatctccggg aatgcgtgag gcagtgcgag cagatgcgcg ggcggctgcc cagtccggac
     7081 cttcagttgg gaatgctcgc cgacaaggcc ggcttctacg atctgctggt cgtgacattc
     7141 ctggaggagt acatggccgg cgggagcagc gccagcctgg actcggcgct ggcgtacctc
     7201 gaccttgccc aggcgcgctc gctggtcgaa agcgccgggg aggcagtcgg cagcccttgt
     7261 cccgaggaga accggcttct ctctcgcctt tccgacctgc agttcagcct gctggtggac
     7321 cgcgatgatc cggccgggct cgggaggtcc cagcgggctc tcgaggattc gctcttcgcc
     7381 gcgcgggtcg aatgcgccgc cgtcagcccg tccgacagcc accccccggc cctgccgacc
     7441 agtcgaaccc tctgctcaac ggttttgccc cgcgatctgg tgctctgctg ggtgctgtcg
     7501 ccggcgggga cgttcgctat cgctctatcg gccgacacag tcgtcgtgcg gccgctggcg
     7561 gcatcgcgcg acgaaatcgc cgaacttgtc cgggagttcg tcgccgccgt ggcggcctat
     7621 ccaacagccc gagacttccc ggaaagcccg taccacgctc ccgggcggaa gctctacgac
     7681 atcctgctga agcccttcgt caacgaacag gacacgaccc gcctgacagt tatcgccccg
     7741 ggtgcgctcg ccggcctgcc ggtgggcgca ctggttgatg cgcacggccg cttcgtggtg
     7801 gagagccggg aagttgtgta cgcgcccgct ctgtcgatgt tggctcggca ccctgaggaa
     7861 gcggagcggt ccgatccccg ccgcatgctg gctttcggcg acgcccggta cgacgacaac
     7921 tcggttctgt ccggacttcc cttctcatcc gaggagcttg accgcatcag ccgggtgtac
     7981 ggcgcgaacg cctcggtcta tgcggactcg gcggccaccg aggcgaatgt gcggcgcacc
     8041 gccgatagct ggacggggat tctgcactta gcagttcatg ggttggtcga tcccgacgac
     8101 tggtcgcgtt cggccctgag cttttcgttc gactccgacg gccggggatc gggcctgctt
     8161 cacgcctcgg agatcgctca cctgaatatt cctgcggccc tcgtgtttct ttccgcttgt
     8221 gagagcggct ccgggaggca gattgcgggc gaaggcgtaa tgagcctggc gcgatcattc
     8281 ctggcggccg gcgccggctc cgtgatatcg accctctggc gcgtcgatga ccgggcctct
     8341 gcggaattcg tcgggcagtt ctacgaccga acggccgccg cggcgccgcc cgcggaagcg
     8401 atcgccgagg cgcaacgcct gatgatccat tccgatcgcc cgctctaccg ccatccgtat
     8461 ttctgggctt cctacgtgct tacccagacg ggcgctcgga agaccaagta agaacagtgc
     8521 gcggagtcgc tccgggtgac cttgcccggc ctccggcaga cctgcatggt cggcggctct
     8581 ctgcgggggc acattcagga cccgtcttat gactcgcagg acggccgtgt ggaggtcggc
     8641 tcgccccgca gcactcccgg agcggcccaa aaacccttgc gatttgactc gcccggcgct
     8701 atcttcgaac ccggtggcgg cgctaagcac agccgatctg gaagaggttc ggttggggag
     8761 acaacacggc gttgtgacac ggcgattgaa ggctatcacc gatgagcgga gtgggacgac
     8821 ggggcggcga gcttttcatt gtcgaaaacg gcgattccga ccggaaagcc tggcagtgtc
     8881 tccacgactg ggccgagaca gctgacaagc tcgatagtgc gacggggtac tgtgagatcg
     8941 gcgctctccc tggacggtat cgaatgctac ccgaatgtcg aggtcacgcc gagcgtgacc
     9001 gtctccctca tggccgagat cgacagagtg atcgaggccc gcggcggctt gccgggggcg
     9061 tttaaggcgg actagcgggg ccgcacacta tcggactgga acgcgggcat tctccggaca
     9121 gcagggggag acacaacgat gagcagcgcc ccgctccggc gggcgaacaa ccgaacctgt
     9181 gacaagggga ccgggacata tgagcgagca ttttctctcc gccctgcggg cgcagctgcg
     9241 cgcgcacggg caggcgcccc tgcgcgtcat cggcatcgac ctgggcacca ccaactgcac
     9301 ggtcgcggag attgtcgccg acggcgcggc tgaggatttc gagccgcggt gtatcgagat
     9361 cgagcaggag acgccgttcc agggtgagta cacgcacatc ctgataccct cgatcgtcgc
     9421 gctccacggc ggccgcgagt atgtcggcga gggggccaaa aggctgcggg ccggcctcgc
     9481 cggcccggag accggcctgg agcgcaatcg gacgatattc tgggaagtga agaacgagat
     9541 ggggatccag cgcacctacg cgcgcgcgcc ggaggggtat cgcggcgcgg ccgaagtgag
     9601 cggtcggatt ctgcggtttc tggccaacag gtaccgggcg gaccacgggg agccgcgcaa
     9661 ggcggtggtg acggtgccgg cttcgttcca acagccgcag cgagaggata cgctccgggc
     9721 ggccaagctg gcgtcgatgg aactggccgg gggcgacctg ctcgatgagc cgctggccgc
     9781 ctttgtcgac ttctggatgc gcggggaatt ggcgaaggct cttccgcgcg gagagggcaa
     9841 tgtcctcgtg ttcgatttcg gcgggggcac ttgcgacacg gccgtggtga aggtccacgc
     9901 gggggacgcc gcgggccacc tgagggcctc cccgctctca gtgtcgcgct actaccgcct
     9961 cggcggcggc gacatcgacc tggcgatctt ctacgaagtc ctcctcccgc aaataatgga
    10021 gcagaacgga ttgagcgagc gcgacctgga tttcggcgtg aagaaaaacc agctcgaacc
    10081 cgcgttcctc ggcattgccg agtcgctcaa agtaggtcta tgcacgcagg tgcggcgtct
    10141 cagggagctg gcgcggggcg ccgaactccc gatcgagccg gtccagaaat tgccggcgac
    10201 ggagcgctgc cggctcacca acggccggga gatcactctg cagtcccccg ccctgacact
    10261 tccgcagttt gaggctgtgc tggagccgtt tctggaccgc accttcgtgt tccatcgcga
    10321 gacggagtac cggttgacct gttcgatctt cgcgccgctt gaggatgccc tcgaccgggc
    10381 ggagctcgac gcgcgggaga ttgattgctg cctgctggtg gggggcagct cgctgatccc
    10441 gcaagtggcc gatgccctcc gcagttactt tgaacccgag aaagtcgtcc tgtactcgca
    10501 cccggatcag gtgcagtcgg cggtggcgcg gggcgccgcc gcgcaggcgc tctcgctggc
    10561 cgtcctcggc cggggggtgc tgcagccggc ctcgcccacg ggcgtgtgca tccgaacaac
    10621 cgccgggaag atgcagttga ttccgcctca cagccccctg ccgtacccgg cggcaggcga
    10681 atgggcgatg aataccaacc tggctctgcc caagcggctg agccagactg aacgcgtagt
    10741 ccggatcgaa ctcatcggga ccgatgattc gctcatccaa cgctggacct ggttggtgcc
    10801 cccggcagcc gctcccggcg aaccgttgac cctccagtac tgcatggacg agaatcaggt
    10861 gatccggtac cggttggact tggaatcggg aaccggtcgg gagggtctca ccgggagcac
    10921 cgagaacccg atccataacg tggtcaaccc cgacctcaag cgaatcagga ttctcgaact
    10981 tgaggagtgc ctccggacct cgctacccgc cggagagcaa caggaggaaa tggtggtcga
    11041 aatcgccaac ttgagccggg agcttcgcca gtatgacaag gcacgctacc tgctggagag
    11101 agttctccgc acccggggcg gcggcgacat cggggttctc aattacctgg gcatagtgtg
    11161 cggcgagcag ggtgaccgcc aggcggagga gaagtactac agagaagccg cccggcttgg
    11221 ttccgatgcc gctcaattca atctcgccta cttctaccgc gaccacggtt cgctggcgaa
    11281 agccgccgag gcgattgaac gcctggtcga gcggttcccc aagggtccgt atttggtgct
    11341 caaggccgac attgccgaac gggaggagcg gatttacgag agcaagcgcg ctttggagat
    11401 ggccaggaag gcgtttgggc cggtgaaatc gctgagcgac tgggaactga gttggtacga
    11461 gcacggcgcc aaacttgccg gcgacgaggc ggcgcaggag atggttcggg cggagaggaa
    11521 acggcggcgg cgctcaaacg cccccgaacc gagcggactc cttccagaaa tgagggaggc
    11581 ctagtttgtg ccatgggcgt ctggctggta cctgaaagcg aactgacacc cgatcagatt
    11641 cgagcggtcc aattgccgac cgacggccac aaggttgttt ccggtccccc gggatcgggc
    11701 aagaccctgg tgcttgttca tcgcgcgcgg tatctcctcg actcaaaggg aatcaccggc
    11761 gggcagctcc gcctgtttgt ctatacccgc acccttcgcg actacatcca gtcggccctc
    11821 aaactgctgg gtattgccga cgaatgcgtg ctcacttttg acgcgtggtg tcgatccttc
    11881 tatctttcgt gcgtgggcaa cccaccccct agggatgagg ttaatggcca gcttgacttc
    11941 agggagatcc gacgtgctgt tctcagagcg ctcgagactg caccggtagc accggtttac
    12001 gattatgtgc tggtcgacga gggtcacgat ctcgacgaga tttcctacaa gatcctcgtc
    12061 cggatcgccc gccacatcac tgtctgtatg gacaggaaac agcagatcta tgacgatggt
    12121 gcggccgagg aacgggtgct tcacatactc gggctgccca agcgcagcgt tgctctcctt
    12181 gaggcgtatc gctgctgccc ctacatcacc cgcctggccg cgaccctcct ggaccgccag
    12241 gagcgacagc cgtatttggg gcaaatgcgg accgagcagg tggagaggga gaagccgctg
    12301 ctgttcatcg ccaatgcaca ggaagcggag cttcacgagc tcgcgcggca gatcagggag
    12361 cgccagaggg tacagggcag gaatgacggc ggtggcggga gcatcgctat cctgtttccc
    12421 caaaagcgca tggtcttcgg atatgcgaac gcgctggccg atctcggcgt acaggttgaa
    12481 gtgcacctgg accggcccgc ccagcaggcc agatatgaaa tggttgattt ccggaacaat
    12541 ctccccaaga tactgactta ccaccaagcg aaggggctca catttgattc ggtgtttatg
    12601 ccgcgcctgg tcggttacgc gttcaaacga gtatctgaac cgcgcttgcg ccggctgctg
    12661 ttcgtcggaa tcacacgggc caagagctgg gtttatatga gcacggagga ctcgcggatg
    12721 ctcccatgcc tcaacgcgat ctgcgatgag tccggagact ttctggctgt ccgatattcc
    12781 ggcgggaatg acgggccgaa gccggacact gacagcggtg aggaagacgg gttggcgaac
    12841 ctcttcacct gagtgactct cggcacggcc gacaaccggc tcgggcggtg gagacgcgcc
    12901 ggcgccggag tgcttccgct tccttgacgg cctcggattc cgcacggttg tgtgaggcga
    12961 ccctttcgcc gggccggctt tcggcgccgc gcgcgtgggc tgcgtcatct gcgtcaggcc
    13021 tgacgcaccc tgtgacgcac ctgcgcgccg gtaactcgtt tcccctcaac acgatttttg
    13081 ccgctgcgtc gcgctgcgtc acgaactgcg tcacaagaca gatccatccg gccgttcaca
    13141 ctcttcgcac ggcgcctctc tttcgcgata actcacctac acagaatgcg atagcgaatt
    13201 cgtgacagcg ccgcccgccg attggcacgc cggttgatat aactgagcgc agcaggcgcg
    13261 cacacgagcc gcagcttcaa catcacctcc cggctgcctc gctgtgtcga cgcctggctg
    13321 accatactta acgcgaaagg tctccgtcgc cgggcgggct tccaagcgag cccgactcaa
    13381 tccggaaccc gcccggcccg ggaccttaac cgccggcgga ggatacgttc cggccgggtg
    13441 tttcggccga tgcgtcatct gatggcgcgc cgcggtcagc gctcccgcgg gagcgcggca
    13501 acctggtgct cacaggcaga caaacccctt tgggagagag ggggtaaggc tcacatgagg
    13561 aggaacacaa acctagattg aaagcgatgg agggccaatg agaccggacg ggtcgcatgg
    13621 ggagaggcga ccggtcggga gcgcggggcg gcaggcacag cacttcgcgc agccgccatc
    13681 ggctccgccg aatcagccag tatctcacgc gagagccact gggagtgttc cctctcattt
    13741 gagcgcgtgt aatcctcgtg cggccggggc gggctctcgg cggcagtgcg cccacaccaa
    13801 ccgccgacgc ccccccggcc cgaggaaaaa aggaggaacg aggtcgttca ctggaaacgg
    13861 aatcagcaag agacgatcgc cagggcggcg ctgactgagt cgcgggcctc agcctgacct
    13921 tgcgttcact cccctcgacc gccggcgcgg aggcatagcc acatgagtca agggatgaac
    13981 gcgacgacgc gagggatgcg ggtccgcggg ccaaggccgg ttgactcggt gcccggccga
    14041 agttccgtcg tgcagcggag gcttgatcac ctttaacccg aggtttatca aggcactgaa
    14101 cgaggtggtt aagtcggctg ttgctcttta tgctagtgcc ggcgttgtcg gcctttgtgg
    14161 tgccgctgcc ggcgacggcg gaaggcccgt ttgatccaca catcgacagc cggccaggct
    14221 gtcacggctg tttcaccgag accgggtgca tctttttcgc cttcttctgc agcgggttgg
    14281 ttatgatcat cgactgggcc catgtgagcc tgatggcgta ctaacctgag gcgggttcgg
    14341 atggaacggg gaagcatgaa ctactggctg atcggctatg aacagaagcg gtcctaccag
    14401 ggcaacctgc tgagggcgct gctgagcacg gcgcttctgt ttatcggcct cggtgtgctg
    14461 atccggttcg cggaaccggg tgcggaacgc cgggtgtcct ggtccgaccg tctgttgggc
    14521 ggggttgcgg ccgagacggc ggcgatccag ccgacgggcc ggggcggcgg agccggcggc
    14581 gggcagggcg cctcccacgc ggcacccttc ctgcggggct acgtcggccc ggttgtgatc
    14641 atccgggacg ccccgagcac gatccgaccg gcggtagcgg tgctgcccgg accatccgaa
    14701 cccgatcctt tgccgtcgcc ggtgccgggc gaaccgggct ggggaggcga cttcgccggc
    14761 cagggggaca atccgtttgg cctgccggcg agcgaaccct acctcccccc ggtcggcaac
    14821 ggcttcgacg gcttcgacgg cttgggcggt gacgggctga actgggcgcc ggcgatccgg
    14881 attcgaccgg cgggctggcc catggacgcc gataacgtgc cccgtgtcga ctccggcgtc
    14941 gtctggctga cgctgacgat ccatgccgac gggcggctga gcttcgtggt cgattcggcg
    15001 tacccggagg cacgcggatt cgtgccgaat gtcgagtggg ccctgataca gagccgggtg
    15061 gaaccggcgc tccaggcggg ggagccggtg gcgacgacct tcgccatgac ctacatcgtg
    15121 cgccggggcg gggtggaaag cgttgttcac agcaagaccg gctacgtgcg ggggtttgtg
    15181 gcggagtgaa aaaataccgg cctgtaaccg gccgacctgt tgcgtctaca aaaacgcccg
    15241 gtgagtcact tcaccgggcg ttcgttctcc tgattgacgg gcaattcgcg ctgttgcctc
    15301 aggccgcggg ttcacgtcgg ccgggagcct gccggcccgg gtttcttccc gcaccgccgg
    15361 tgaacgccgc gcgggcggcc gccggtgcga accggcgcta gtcggcggtc ggcgacacca
    15421 ccgaggtgga gtccgggcag tcgacgatcc agattccgcg cagcggcagg gcgggcggag
    15481 tcggagcgct gagattgccg aagatcccgg ccatgcatcc ctcgcgccgc ggctcgccgc
    15541 ccatctcata gaacacgaaa cggccgcgaa accacccgtg gccggccccg caggtcggac
    15601 acatccgcgg gtcgtcgtac tgccagatgc cgctcacctc gccgatgacg acatcgagca
    15661 gcacgccgct gatcgagccc gcgtagacgc ggtccccctc cggggtcttg cgatagactg
    15721 cagacatgac accgatcggg gtgttggtgc ggtcatacca gaggccgctg aaatacccgc
    15781 tgtcgcccgt gttgctctgc agcgtccatt cgccggagat gaaaccgcgg gggcaggcgt
    15841 ggtgccagat ccggcgggcc ttgacggcaa cgacatgggt gtggcccacg atgtagaaag
    15901 cggtgaagcg ggtcagttgg tcgcgggtga gcgcaagctc gcacagcggg gttttgaacc
    15961 ggaccctggc gttcgcggcg gcgtcgctct cagggtcgag aaacaccagg aacgacagcc
    16021 catcgagatc cccctcggtg aacgacgtcc acagggcggt tggggaactt gcggacggca
    16081 gcactgaatc ctggccgggc tcgaaatcaa tcacggcgac gacgtcaagc aggccagcgg
    16141 cgttttcgat cgccagcgat cccgaccagt cggtgggcgg aacaaccgcc gcgggcccaa
    16201 gcgggaattc tccccacacg aaagtgacag cgtaggcatc ccaggccgtc cagggaagag
    16261 cgcccggccc cgtgaattcg ggatagcgca gcgcggccga cgcgacatcg ccctggtccg
    16321 ccgcatggcg gtcgaggacg gcggcgaccg ctcccggcat ctcgcccggc cgcatggccg
    16381 actggtccgg cgccgacgga cgctgcccat cccggctgca cccggccgcg ctcagggccg
    16441 cgagcaccac aaacaggagc acacgtttca tgatggtcac tccctccggt tacgattagt
    16501 tcagcgcgac tgcgaacttg accatgtcgg actcccggtg aacccggtcg tcggaccctc
    16561 cggtcgacga cgggggaggt aaatcgacat tacctgtcca caaaatagcg gtcccggcaa
    16621 tgtcggacaa gcgataaacg cgccgccgcg cgcgatgagg gtgacagaga ccgtcggagc
    16681 gcggtggcga gagcgaaatt tgagtgaaac tgccgacttt gtggtatttc ggttgccctc
    16741 aatttgcgcc ggtcggagga atgcccggtt tgtcttgaca cggtagatca gagtgtctat
    16801 cttgcaacta gttaaagagg atgcaatcaa catgtggtgg cattccggcc gcacttcatc
    16861 gacccgcccg ctcgggttgg cttcttccct cgccggcccc cttcgggaga ccagcggcgg
    16921 gggtgagatg cgcccggcgg accatacccg acggaaccca gcattgtcga cgtggagccg
    16981 tgcgcatgcg acgaccggag aatccggccg ttgcgacggc cgcttttgcg tgcccgtgct
    17041 tgtgaaccag ttcacaagct tcggagcgac tcgtcgaccc gcggggaccg gagcgcgggg
    17101 cggtccggcg gactgaggcg ccggtcgaaa tgacgccatg agtgaaccgc cgcccgcggc
    17161 ccggccttcg aaccgacccc gccgcgccgc agcggaccag aggagaccga acgatatgga
    17221 aaagtggcaa ggttgggaca agattcgcta cgagtcggag cttgaccacc acttcgccga
    17281 cgaaatcgcc tcgattccgg gcggcgacaa gctcttccgc tgtatccagt gcggaacatg
    17341 cagcggcatg tgcccgctga gttcctacat ggattacacc ccccgtcaga ttgtcgcgat
    17401 gatccgtgcg ggctttcgcg gcgaggtgct gtcgagttac acgacctggc tgtgcgcctc
    17461 gtgttacgcc tgtacggtcg aatgtcctca ggagatcaag atcaccgaga ttatgtatgc
    17521 ggccaagcgg ctggcgattc gcggcggcat gcacccgaag cgttttccca gccaggtgct
    17581 cgcgaccgag tttttccgcg gggtggagcg caagggccgc agcaacgagg ggccgctgct
    17641 ggtgcggctc tttctgagga ccaacccgtt caagatgttc ggcaacaccg ggctcgggct
    17701 cggcctgttc cggcggggac gcctctcgcc caagcttgag tcgatcaagc gaaccggcga
    17761 gctccacacc atgctcgccg cgattgagca ggagcgcatg gtccgccgga cggagacaac
    17821 ggaggcgacc gcatgaaata cctctactac cccgggtgct cgctgaagag caccggcaag
    17881 tcctacgagg aatcgatcct cgccgtgttc tccgctctgg ggcggccgct cgaggagctg
    17941 gacgactgga actgctgcgg ggcgacggcg tacatgtcga tttcggagat gaaggccttt
    18001 gcgctctcgg cgcgcaattt cgcgatcgcc gagcgccagg gagacagcga cacgcatctc
    18061 gtcgtgccct gcgcggcctg ctacctcggc ctgaacaagg cccaccgcta tctcgacgag
    18121 caccccgaca tacgcaagac catccagagc gcgctcgccg ccgccggcct gagctacgag
    18181 aacgggatca aggtgcgcca cccgctcgac gtgctcgcca acgatgtcgg attggaggcg
    18241 atcaagggag cggtgacccg gccgctggcc gggctgaagg tggcctgcta ctacgggtgc
    18301 cagctcgtgc ggccttatgc cgacttcgac gacgcccatg agccgatggt gatggaccgg
    18361 cttatcacgg cgctcggcgg cacgcccgtg gcttggccgc tgaagacgcg ctgctgcggc
    18421 ggctcgctca ccggcacggt gccggaggtc ggactgcggc tgagctacgt tctgctgcgc
    18481 gaggcgcaga agcgcggctg cgacgtgatt gccaccgcct gcccgctctg ccagttcaac
    18541 ctcgagtgct accagccgaa gatgagccgg atgtacgaat cgcgggtgaa cgtcccggtc
    18601 gtctacttct cgcagctcat ggggacggcg ttcgggctcg accggcgccc gctcgggctg
    18661 cagcggctgt ttgtcccgtt tgcgtaccgc gaacccgcgg ccgcggtgga aggaggcgcc
    18721 catgtcggct gaccagtcct cgaacggcac gcccaaagtc ggcgtctacg tctgccactg
    18781 cgggatcaac atcgcccaca cggtggacgt gaaagcctgt accgatttcg ccgcctcgct
    18841 cccgtacgtc acggtggcga aggagtacaa gttcatgtgc tcggacccgg ggcaggagat
    18901 catccagcag gatatccgcg agggcaaggt gaaccgggtg gtggtggcct cgtgctcgcc
    18961 gctcatgcac gagaacactt tccgcaaggc gacggcggtg gggggcggca acgccttcat
    19021 gttccagatg gccaacatcc gcgaacacgt gagctgggtg acgctggata cggcgatggc
    19081 gacggagaag gcgaaagcgc tcatcgcggg ggcggtcacc cgcatcgtcc gccacgtccc
    19141 gctccagcgc aaccgcgtgc ccgtgcaccc cgacgtgatg atcgtcggcg gcggcatcgc
    19201 cggcatccac gccgcgctca cgatggcgga cgcgggcaag aaggtctacc tcgtcgaacg
    19261 cgagcccact atcggcggcc acatggccaa gttcgacaag accttcccca ccctcgactg
    19321 ctccgcctgc atcctcaccc ccaagatgtc ggccgtccgg tcgcacccca acatcaccct
    19381 ctggacctac gcggaggtgg ccggggtcga gggattcgtg gggaatttca aagtgaaagt
    19441 gcggcgcaaa ccgcgctacg tgatcgagga gcagtgcgtc ggctgcctcg actgcattga
    19501 cgcctgcatc tacaagacgg ccaaggtgcc cgacgaattc aacgtcgggc tgagcaagcg
    19561 caagccggtg tacattccct ttccccaggc caccccgctt gtcgccgtga tcgatccgga
    19621 aacctgcatc gagttcaaga cccaccggtg caaaaagagc tgcctggagg tctgtcagaa
    19681 gaacgcgatt gatttcagcc agacggagaa gatcgaggag gtgccggtcg gggcgatcat
    19741 tctcgcgacc gggttcaaga ccttcgacgc ccggcggatc gcccgctatg gctacggcaa
    19801 gtacccgaac gtctactcca gcctcgagat cgagcggatg gtcaacgcgg ccgggccgac
    19861 cggcggcgag gtgaccatgc cggacggcgc caaaccgaaa gcggtcggca tcgtccactg
    19921 cgtcggtagc cgcgacgccc acaccaaccg ctactgctcg cgggtgtgct gcatgtactc
    19981 gctcaagctc gcccacctga tcaaggagcg gacgggcgcc gaggtctaca acttctacat
    20041 cgacatgcgg acgcccggca aggggtacga ggagttctac gaccgtctcc tgcaggagaa
    20101 cgtccacttc atccgcggcc gggtggccga gatctcggac tgggcgatga gcgccgagga
    20161 ggaggggaag ctcacgatcc gggtggagga cacgctggtc ggggcggtgc ggcggatccc
    20221 ggtcgacatg gtcgttctgt cggtcgggct ggagcctcag gcggacgccg acgaggtgcg
    20281 gcggcagttc aacatcagct gcagcacgga ggggtggttc ctcgagcgcc atcccaagct
    20341 ggcgccggtg tcgacgttca ccgacgggat ctttctcgcg ggagcctgcc agggtcccaa
    20401 ggacatcccc gactgcgtcg cccaggcggg cgccgcggcg gccgaggccc tggcgctcat
    20461 cgaccgcggc cacgtcgaac tggaaccgaa caccgcctac tcgcaggaag aactctgctc
    20521 ggggtgccac acctgcatcg ccctgtgtcc ctacagcgcg atcgtgttcg acaaggcgaa
    20581 gaacgccgcg atcgtcaacg gcgcgctctg caaggggtgc ggcacgtgtg tggccgcctg
    20641 tccctcgggc gccctccagc agaacctctt caccgacgag cagattttcg acgaaatcaa
    20701 gggagtgctg agctatgtct aagaccgaat cgagccggaa ggaattcgaa ccgcggattg
    20761 tcgcgttctt ctgcaactgg tgcacgtata cggcggccga tctcgccggc acggcccgca
    20821 tgaagtacgc cgggaatgtc cgcgtgattc gcaccatgtg ctccggacgc ctcgatccgc
    20881 agtttgtctt cgccgcgctg cggcaggggg cggacgcggt tctcatcggc ggctgccacc
    20941 ccggcgactg ccactaccag gaggggaact acaaggcgct ccggcgggtc gtcctcatga
    21001 agcagttcct cgcggccatg ggaatcgagc cggagcgcgt ccgcctggag tggatcgccg
    21061 cctccgaggg ggacaaggtc cagcgggtga tcaacgaaat gaccgaggcg gtccgggcgc
    21121 tcggtccgct tcgccttgaa cagcggaccc tgctccacgc ggcggaactg cccgccgggc
    21181 aggccgggcg caccgcggag ccggccgggg cggaaggagg acggcgatga gcaagccgaa
    21241 agtcgcgttc tactggtgcg ccagttgcgg cggctgcgaa gaagcggtcg tcgacctcga
    21301 cgaggccatt ctcaaggtcg tcgaggccgt cgacatcgtc ttctggccgg tcgccctcga
    21361 tttcaagcgc accgacgttg aggcgctgcc cgacgggtcg atcgcggtca gtttcatcaa
    21421 cggcgccgtg cgcagctccg agcagttcga gatggtcgaa atgctgcggc gcaaatcgca
    21481 actggtggtt gcgttcggaa gctgcgcgca cctcggcggc gtgccgggac tcggcaatct
    21541 gtaccggcgc gaagagctga ccgccaccgc ctaccgcgag gtgccgagcg tgaacggcgg
    21601 caacggcgtc gagcccgcgg tgtcctcgac ggtgccggag ggaacgctca ctctgcccga
    21661 gctctggaag acggtcaaga cgctcgacca gacgatcgcc gtggactact ttctccccgg
    21721 ctgtccgccg ccggtgaagc tgattctcgg cgcggtggaa gcgatcctcg cggggaagct
    21781 cccggccaag gggagcacgc tcgcgccgga catggcgctg tgcgacgagt gtccccgcaa
    21841 ggagaccaag ccggagaaac tgacggtgaa ggagttccac cgtccgcacg agattctcat
    21901 cgatcccgag cagtgcctgc tcgcccaggg gctcctgtgc ctcgggccgg tcacgcgcca
    21961 gggctgcggc ggcgcctgca ccaaggtcag catgccctgc accggctgcc tcgggccgac
    22021 cagccggatc accgatttcg gcgccaaggc gatgtcggcg atggcctcca tcatcgaggc
    22081 gaaagacgac gccggcatcg aagcgacgct cgccaggatc gtcgaccccg ccggcacttt
    22141 cttccgctac agcctgccgg cctcgctcct ccaccggaag tatgaacccg aaaccacgcg
    22201 ggggatgaaa tgacgacgac acgacgggtg acaatagacc cgatcacgcg gctcgagggg
    22261 cacggcaaga tcgacattct cctgaacgac cggggggacg tcgaccatgc ctatttccag
    22321 gtgcccgaac tgcgcgggtt cgagcggttc gcccacgggc gtccggccga agacatgccg
    22381 cagatcacct cccgcatctg cggcgtctgc ccgaccgccc accacatggc ctccacgcgg
    22441 acgctcgacg acctgtacaa ggtggagccg cccccggccg cccacaaggt gcgcgagctg
    22501 ctctataaca ttttcttcgt cgaggaccac gccctgcact tcttctacct cgcggggccg
    22561 gatttcgtgg tcgggcccac cgcccccaag gccgagcgca acatcatcgg cctgctcggg
    22621 aaagtgggga tcgaagcggg caagaaggtg atcgggatgc ggcgggacct gcgggagctg
    22681 atggtgctgg cgatggggaa agccgcccac ccggccttcg gtctgcctgg cggcatctcc
    22741 cggccgatcc cgccggagca ccagaaggcg ttcatcgagg tgtgcgagcg ggcggtggaa
    22801 ttcgcccagt tcagcctgaa ggtgttcgat gacgtcgtgc tggcgaacaa gacgtacgtc
    22861 gactacatca cctccgacac ctacactcac cggacccact acatgggcct ggtcgacgcc
    22921 cacaaccgcc ccaactacta ccgcggcgaa ctgcgggtgg tcggtcccga cggccgcgag
    22981 gtgtgcaagt tcccggtggc caaatacctc gaccacatcg ccgaacacgt cgagccgtgg
    23041 agcttcatga agttctccta cctgaaagat gtcgggtgga aggggttcgt ggacggtccc
    23101 gacagcggca tcatcgcggt ggctccgctg gcccggctca acgccgccga ggggatgccg
    23161 acgccgctgg cgcagaaagc cttcgagcgg ttctaccaga cgttcggcgg gcggccggtg
    23221 caccacacgc tcgccaatca ctgggcccgc ctgatcgagc tgctgtgcgc cgccgagcgg
    23281 gcgcgcgagc tgtcccgcga ccccgagatc gtgagcaagg atgtgcgggt gctcccgacc
    23341 aagacgccga gcgaggggat cggcgtggtc gaagcgccgc gcggcaccct cattcaccac
    23401 taccggaccg acgagcgggg cgtgatcaaa atggccaacc tcatcgtggc gacccagaac
    23461 aatgccgccc ggatctccct gtcggtggac aaggccgcgc gcggcgtgat ctccggcggc
    23521 cgcatcgacg acggcgtcct caacctcgtc gagatggcct tccgggccta cgatccgtgc
    23581 aacggctgcg ccacccacac ccttccgggg tcgatgccgc tcgtcgtgcg cgtgtacgac
    23641 gccgcccaca accaggttca ggagattcgg cgcgactgat gatgaaaccg gttgtcatgg
    23701 gcatggggaa cgagctgttc ggcgacgacg ggttcggcat catcgccgcc cgccgtctgg
    23761 cccccctggc gggcgaggcc gccgatgtga tcgagtgcgg cgccgccggt ctggcgctgc
    23821 tggacccgct catcgggcgt ccccgggccg tgataatcga tgcgatttgc acggggcggc
    23881 aggccccggg cacgatatat cgggtcagtc cggcagactt gcgggacata accaacctct
    23941 caccgcatta tgccggactg cccgaggtca ttcgcatggc gcagcggctg gagctgccga
    24001 tacccgacac gatcgatatc atcgcgatcg aggcggcgga catcacgacc atgggcgccg
    24061 gtctgagccc cgcggtcgcg gcagctctcg aaccggtcgt cgccctggcg gtcgagctcc
    24121 tgttgggcgg gcggcggacg ggcagcccgg cggcggacgc gcaggggatc tccgcggccg
    24181 gcacacacgt ctgaaacccg cgcccgcgcg gcggggcggg cggcggtttt ttttattttc
    24241 cgcttgacac ggcggcgggg aacatgtacc gttaagctcc agaatgtatt atttccgcac
    24301 agatcaaagc gtttcctcat cggcggccgg ggcgcgcttg cgcccggtcc gcgaccgcag
    24361 cctatgcgcc tgccgctgaa gagaccggac cggcgcccga cgggccgccc tgcggcaagg
    24421 gcgcgcgagg cggtccaatg accgccgccc gcgccgcggg ggttttcttt cccgcacgcc
    24481 aactatcggc cacccggctt tccgggcggc ctttttttat caccgccgta tgccatggag
    24541 gcccgtatgc gtcaccacgc tgtcctgcag ggcacgtatg cccagaccac gcttctgcgt
    24601 tccgcgcttt tgaaggccgc ccgcgcctgg ttcgagcgcg agcactttct tgagatctgc
    24661 gtcccccaca tcaccggcgc caccggctcg tgcgagtggt tccccaacgc catgccggtc
    24721 accatgtacg atcccgcggg cgccgagacg ccgatgtttc tccgccagac cggccagctc
    24781 tacctcgagg cgttcaccca ggcccacaac cgcgtctata cgatcggacc gtcgttccgc
    24841 caggagcgca aagtgaccga ccgccacctg tgcgagttca ccctcatcga gttcgagggg
    24901 cgcgactttg agctggacgg tctgatgaac cacatcgaag gtttggtcaa gacgatgtac
    24961 cgggccgctc gcagggtcat gccgaccgac cggctcaccc gctacatccg gtctcccttc
    25021 aaccgcatca cctaccgtga ggcggttcgg ctcctttgcg cccgcggcca cgggctgact
    25081 tacggcgacg acctcgggag cgccgaggag aaggcgctgt gcgcccacct cggcggtctg
    25141 ccgaccttcg tcacccacta cccggccgat cccaggccgc ggcccggcgg cgtgatcaag
    25201 ttcttttcga tgaagcggcg ggagggcgag acgctctgct gcgacctgct tctccccttc
    25261 gtcggcgagt ccgtcggcgg ggccgtgcgc gagggatgcg ccgagacctg ccgccggcag
    25321 ttccgcgaat cgtacatgta cgaccacatc gtcggcgccg ggatcgaccc gcggcagttc
    25381 gactggtact tcgaggtgct ctcgcacggc accggccaga gcgccgggtg cggcatcgga
    25441 ttcgaacgcg tcgtgcagtc gattctggcc acccgccagc aggaggtgac catcaagggg
    25501 gcggtggaac tcccccgctc gcccgatttt cttgtcccct gacgaccgtc ctgctgacgg
    25561 cccgcgccgg ggaggcggcc ggctattcgc agatcggcgg cggaccgccg gcgaacaggt
    25621 acgccacgat gtacgtgacg tcgctgattt tcacctgccc gtccccgttc atatcggcgg
    25681 aggaggggaa agcggctccc ggaccgcccc ggaacttgaa gttcacaaag aacgtcaggt
    25741 cggagacctt gatgctgccg tcgccggtca catcgccgca gtagtagtag gtgacggccg
    25801 ggcgggagtg cagttcggtg acgaaatcat agtaggcccc cacggtcacg gtgagggtat
    25861 cgtcgggggc gccgtagagg ggggcggtca ggacacacgc gaaggttccg tcgccgttgt
    25921 cggcgaccgc gctcagaacc gcctggcccg tcgtgcccac ccgcaccgag tcgatccgga
    25981 ccgggggcgc cccggcccag ttgcgcggag caatggtgat gaccgtcgac gcctgcccgt
    26041 cgccgtcgag cgcagtcttc ccgatcacca ccgtcgagga atccgggtgg gcagcctggc
    26101 gcagacgcca ggtgtcgaac tgggcgtgca gaacatcgat cgggtcggtc gatccggtag
    26161 tggtggggac gatctcctgg aggaaaggcg tgccgccgtc gcccggccgc acgaccttga
    26221 tgaatgccga gatcgccgac ttgccggcga agatgcagcg gttgtccgcc cccggccgtt
    26281 tggccgcctc catcgccgcc atcagtttcg cctccagcgg gccgtcagtt cccagaaacg
    26341 ccgtctccat gtcggcgatc acctccggcc cgaggaggat gttcccctgg acagcgtagc
    26401 cggggccggt gaggtgaccg gcccagtcgc tggtagcgct gccggtgtag gcggccgagg
    26461 cgccggatcc ggccagggtg acgacgccgt actggcggta ttcgggcgtc ccttcgacat
    26521 cgttctgaac gagcaggagg ataatcgaat cgggcgtcaa tccctgggcg agcaactggt
    26581 gggcgtagtt cttgttgccc tgcagccagt aggcctgggt gtggaccgcg ccgatcgatt
    26641 cgatgatgtc atcgatgatg aagcagttgt cgatgcagga ggctccggcg ccgccaatga
    26701 cgccgttgac ggtatcgacg gccacgattg aaaacgtcgc ttccgccggg gcggcggctc
    26761 ccgcgacgag ggctgcaacc gcccacgcgg ccgacaccag tctccgattc atacgcactc
    26821 ctgggcaagc ggacggtgat gggacctttt ctccaatatg cgcggatttc gcgattcgtc
    26881 aaggcctctt cttgccgcgc gcgttccccc ggcgtatatt ggcggaaaag gtcgggtgac
    26941 cgagggatct tcatggagcg acgcgagttc ctcaagcgaa cgggccgggc gatggtgctg
    27001 gcggccgcct cgggcggcat cggcgtcctc ttccacaacc gcaacgccgc gggttacaga
    27061 ccgctcgcgg tgttcgaccg ccggttcacg gttttcggcg acccggcctg ccccgcggtc
    27121 gcttttgccc gccaccccga ccccgagcac gccctggacg ccgccctggc cgcggtcggc
    27181 gggatcggcc gtttcgtcaa gccgggagag accgtcaccc tcaagccgaa cgtcgggtgg
    27241 gaccgcaccc cggagcaggc agccaacacc agcccggcgc tggtggcggc gatggtccgt
    27301 cggtgcctgg gcgcgggggc ggcggcggtc gtcgtcaccg acatgtcgtg caacgatccc
    27361 cggcggtgtt ttctccgttc gggcgtgcgc gatgccgcgg cgcaggccgg ggcccgcgtt
    27421 accatccccg ccgccggcga cttcgtcgag gtggatctcg acggcgagct gctcaccgcc
    27481 tggccggtcc tccgggcgct cctgcacacc gaccgcctca tcaacatgcc catcgtgaag
    27541 caccactccc tcaccgcctg tacgatcggg ctgaaaaacc tctacgggat tctgggcggg
    27601 atgcgccacc gcctccacca gcaaatcgac cagtcgctgg tggatctcac ccgattctgc
    27661 acgccgactc tgacggtgat cgacgccacc cgcgtgctgc tgcgcaacgg gccgcagggg
    27721 ggatcgaccg atgacgtggc ggttgagaac gccgttctct gcgcgaccga tccggtggcg
    27781 ggcgacgccc gcggctgcga actgctcggg ctgacggccg accgtgtccc ccacctgctc
    27841 cgggcggagg cggccgggct cgggcgcatc gactaccgga atgccggtta ccgggacgtg
    27901 acggcatgac gatcgcggcc gtccgccacc tccgccgcgc cagccaggcg ctcttcttcg
    27961 cggcgttttt ctggctcatt ctcaagacca cgttcgaggc ggatttccgg gcgacgggcg
    28021 gcggggagat ccgtctgccc taccccgtct cgatcgggct ggagctcgat ccgctggtcg
    28081 gcctggccaa cctgctggcc aacgggacgg tctactccgg gctgctgctc gggctggtgg
    28141 tgttgcttcc gacgatcttc ttcggccgct tcttctgcgg ctgggtctgc ccgctcgggt
    28201 cgctcaacca ctggctgtcc gaggtgcgct ccgagcgcgc ggcgaaccgg ggggcccgga
    28261 agatcgaggc caaccggtac cgcggctacc agcgcatcaa gtactatctc ctgctggttt
    28321 ttctgggcgc ggcgctcgcg ggcgtactcc agatcggtct gcttgatccg atcgccctgc
    28381 tggcccgctc gctcggcacg gtggttctgc cggcgctgca cgtggcggcc gagggcgtac
    28441 gggacgcggt cgcggctgtc ggcttcgcgc cgctgacgaa cgcggcgcag cgagtctacg
    28501 atacgctggg gccgctggtg ctgcccttcc gcccccagtt tttccatgcc ctcctgacga
    28561 tcggggcgct gtttgtggcg atcctgtggc tcaaccggct cttcacgcgc ttctggtgcc
    28621 ggggaatctg cccgctcggt gcgctgctcg ggctgttctc gcgcttggcc gttttcggcc
    28681 tggaaaaaaa cgccgcctcg tgcgaccact gcaacaagtg cctgctccac tgccaggggg
    28741 cggacaaccc ggatgtcggg agcgcctggc ggcaggaaga gtgcgtgctg tgcctgaact
    28801 gccaggcagc ctgtccgcac gggtcgctcc gcttccggtt ctttccccgg ctggagggga
    28861 cgcgcgacaa cccggcgccc gtccggacgc cgcacctcac gcggcgcaaa gtgctcgggt
    28921 cgctcgccgg cgggctcgcc ctgttcccgc tcttccgcgc cggcgacgcc ttctcggcca
    28981 atcccaaccc cctcctcatc cgtccccccg gcgccccgcc cgaggacgac ttcctcgccc
    29041 gctgcatccg ttgcggccag tgcatgcgcg tctgtcccaa caacgccctc cacccgactc
    29101 tcctcgagag cggtttcgag gggttgtgga cgccggtcct gatcccgcgc atcggctact
    29161 gcgagccgag ctgcaccctg tgcggccagg tctgcccgac cggcgccatc gaagagctca
    29221 ccctcagcga gaaagtgggc gaccgggcga ccccgggcgt ccatatcggc accgcttttg
    29281 tcgaccgcgg ccggtgcctg ccctgggcca tggccattcc ctgcatcgtg tgcgaggagt
    29341 ggtgcccggt gtcgcccaaa gcgatctatc tccgggagga gattgcctac gaccggaacg
    29401 gggacgcagt gaatgtcaag cggccctttg ttgatcccgc gatctgcaac ggctgcgggg
    29461 cgtgcgaatt cgcctgcccg gtggcggatc aggcggcgat tcgggtgagc tcggtggggg
    29521 aatcgcgctc gagagaaaac cgcattctgc tggaccgtcg ccccgcccgc cgggagccgt
    29581 ccggaccgcg cgccaactga cgcattcgcg gcggccccct cccatgtcgg cccggcggag
    29641 agtcttcgct agcggcttgc ccagaacacg ctgagcgcct cgatcatcgg ccggccctct
    29701 tccccccaca ggtcgggggg ctggactttc cacccgcaat gcggatcacc cttgccgatg
    29761 caggtcggtt ccatcgccag caccggccag ccgcagaagg ccgagcacca gcccgtggcg
    29821 tagccggtca gagtccagca gaccgactgc gggctcggcg ggttgaaagc caggtgctgc
    29881 tccgcctccc aggagttggt ccagatgccg gcgaattgga attcccggct gtgcctatcg
    29941 aagcggatct cggtcggggc ggccttcaca atcccctcgt aggtgtgcag caccggtccg
    30001 gcgcacagga ggtccatctc atcggcgaac tcgaagttgt gccggatctg catgaagtcg
    30061 gagtagccgt gctggtagcc gaaccgcagg agaaagtcgc gggcctgcgc ccagccgaga
    30121 gtgtcgagga ggttctggcg gagcatgccg atcgcgttgg cgtcgaggat caggacgcgg
    30181 ctgtcgcgaa agagggtgcg cccggtgtcg ggatggaagc tcagttcgcg ggaaaggtcg
    30241 aacccggcct cgcccgcggg cggcgcgccg gcggccgcgc ggcggaggcg ttccccccgc
    30301 acagtgaccc agccctcccg ccggcccgcc tcctggggcc gtcggccgcg ggcggccggc
    30361 cggtttcttt tggtcgaaac gatgtccacg cctctcttat cggctcccga taggccgaat
    30421 ttgactccgg ccccgacgga acttgcctga cgctccattt ctgtgtaatc ttggctccgg
    30481 aaaggacgtt atgaacaaca gcgatcaact cttcaccgcc gatctgcact gtgacacgat
    30541 tcaccggatc ctccagggcg ccgatttctc ccggcgcaat gagatcggac acctcgatct
    30601 tccccgcctg atcgaaggcg gaatcgatct gcaggtattc gcctgtttca cggagtcgtt
    30661 catcgaggcg aaggaccggg tcccgctggt ccaccggatg ctcgacgggc tgcaccgcac
    30721 cgtggaggcc catggggaga aggtcgccct cgcccggacc tcatccgagg ccgcgcgcaa
    30781 ccgcgccgcg ggcaaaatca gcgccgtctt ctcgattgaa aacggactgg tgctcaacga
    30841 caacctggac actctccggc agttctatga gcggggcgtc cgaagcctga ccctgaccca
    30901 caacgtctcg agcgcgtggt gcatttcctc ggccgatgag cacccggcgt tcgacggtct
    30961 gactgctttc ggacgggagg tggtgcggat gatggacgaa ctcggcatga tcgtcgacct
    31021 ctcccacgcg gccccgtcgg cggtggcggc cgtactggag atcgccaccc ggccggtgat
    31081 cgcctcccac tcgtgcgtcc gcgctctctg cgaccaccac cggaatctca ccgacgagca
    31141 aatccgggga atcgccgcga ccggcggcat ggtcggcatc aacttcctct gcgattttct
    31201 ctccccagcc tgccgcgacg cttccgccgc ctacatcaac gcccactatg atctgggcag
    31261 gagatactgg ctgctgttca cggccgagtg cggcgccgag gagtacgcgg cccggcgggc
    31321 ggagctgcag ccgtttcttg cgggctggga gcaggtcgtg cgggccacgg gggtcgatgt
    31381 gggggtggtg gccgaccaca tcgactacat tgtccgcctg gtcggcccgg accatgtcgg
    31441 cctggggtcg gacttcgacg ggatcacgtt tgccccggcc ggccttgagg attgtgcgaa
    31501 aatgccgcgt ctggccgacg aactgcgggg gcggggatat tgccgggaag acctagctaa
    31561 gatcatgggg ggcaatttcc tccgcgtttt ccgcgaagtc tgcggctgac ccgaccggaa
    31621 cccccggccc cccttttcgg ttgaacacca cggcggcccg cgttatatta agggcaagtc
    31681 gggatccgcg ccgacgacct ggtcttctca ccggcaaccg tcacgtgtac cgcgggttta
    31741 cagatttcaa aaccgttcga taatgtatag gaacaaggca cttgccggcg gatcgcggcc
    31801 ggcgtcgtcg gaccaccatc ggaggattgc cgtgtctctt catcgatccg ctcgccgcgc
    31861 ggctctgccg ctctttttcg gactgcttct cgccgctccc tatgtttccg cacaaatcag
    31921 cgaaggggga acgcctccaa gccggatggt ccgcgccggc gctcttgccg ccgcaacctc
    31981 cctccccacc gtggataccg gacccgtcga tgtcccggcc ctgctggcgg aggatgcggc
    32041 cgaacaggac aagggcgtcc cgttccgttt cggctacccc ttcgagacgg tctgcgacct
    32101 gaccaattcc gggcagtggg acacgctctc cgacggcggc cggctctggc ggctccgcat
    32161 cgtcgctccc ggcgcgtact cgatcaattt ggtgtatcgg aaattctggc tgccgccggg
    32221 cgcaaagttc ttcgtgtaca gcgaggacta ccgatacgtc atcggcgcct tcaccgaacg
    32281 caacaataag gagtacggcg agttcgccac ccagccggtt cccggatcgg tgagcatcct
    32341 tgaatactac gaacccccga acgcccctga gccggggatc atcagcctcc agcggatcgt
    32401 ccacgcctac cgggatatct tcgcgacggc gacgatgaaa gaggcggccg gattcggcgg
    32461 gtccggttca tgcaacaaca acgtcaactg cccggagggg gatctgtggc aggacgacaa
    32521 acgggcggtg gcgatgatcc tgacttccgg cggctaccgg atctgcaccg gcgcgctggt
    32581 caacaacgtg cgcgaggatc tcaccccata cttcctcacc gccaaccact gcctcggcgg
    32641 cgagagcacg tggatcttca tgttcaacta cgagagcccc tcctgcgcca acatcgacgg
    32701 ccccacctgg atgaccgtgc agggatcggt ccgccgcgcg aactactcca gttccgactt
    32761 cgcgctcctg gagctgctcg aacagccgcc cgattcctac aacgtgtact acgccggatg
    32821 gtcggccgtc gacgtggcgg cgcagagcgc ggtcggcatt caccacccct ccggcgacat
    32881 caagaaaata tcgttcgact acgacgcggt ggtgagcgcc aactacgcgg gcagcaccgg
    32941 ggggacgcac tggcggatat tgcagtggga cgacggcacg accgagccgg gttcctccgg
    33001 ttcccccctg tttgattcgc tgacccaccg catcgtcggc cagctccacg gcggcacagc
    33061 ctcgtgcgcc agtctgacct ccgactacta cggcaaggtg gcgcggtcct ggacgggcgg
    33121 ggggacctcg acgtcgcgcc tgagcgactg gctcgatccc gacaacacgg ggacgctggc
    33181 ggtcgacggt ct
//
LOCUS       DAOPOE010000023        32087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1827_length_32087_cov_5.560783, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000023 DAOPOE010000000
VERSION     DAOPOE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 32087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32087
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1827_length_32087_cov_5.560783"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2693
                     /locus_tag="PKM94_04980"
     CDS             <1..2693
                     /locus_tag="PKM94_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66028.1"
                     /translation="GQFIQRQIVISQPDLQGLYIFDLVVPDYNPEWVSVDVQGFNFQM
                     LEMEPNFIIHTCKGSLDLSFVITGGAASADTCDYYKPRQLDYAPEGMPDFDMKQAGWT
                     DQLGRWSHDGPAALANCLWWFDSKFEPNPVDPRPFGMVPPNDGYPLVTAYLPTDDHDP
                     TNVQPFITDLAANYVQTNVGGTGTQPGYLQTGFKNYLAVRGLAGQYRDTAVLAPTFEY
                     IQRQVLDCQDVILLLSFWEDRGAAAASYIGSHYVTVAGVCTDLAQRQMCISDPWLDAL
                     EGEPPAGSAHIGTVHNDADNISGPHSQIQHDPYQCMSATPPGFTGPVEVVNYPADAMT
                     LNNFYGMNLNIDPTPYGGGQVYTVITEALVICPHTVQPIDTCEYYKLPYEDYAPYGMP
                     DFDQKQDNWRYPPSGAWSHCGPVALANCFWWFDSKFEPSPVDPRPFAPSPISPPWNDN
                     YPLVGIYPVAGAWDDHDTNNVKPFVDSLALYAQTNVGSSGTSVLNLATGAQNWLASVG
                     LGTRYTMVVMPLDPAVPYEFIRDQVLESQDVILLIGFWQEMQTGYCERIGGHYVTVAG
                     TCPDPLDSAFCISDPYFDNNEGEPPAGSAHSAGAHNDAANVSGPHGTIHHDRYDVMPA
                     ACQQFVGAPLFTMELAGYPTATADIAPFYGQNPFDPSLPPVPPQPGLPVHAILEWAII
                     ICPQPPSGADTVKCEPQGGTNPNHPNSYWYDVKPGNPTGLFDFHVKVLDTIPRYYLNW
                     LEPAGWLHQLHKVGADWWVSWWDPTGGNPITATFRFGFDNWNPSAWNDWVTTTSASNN
                     PAAGVSDNSGNHVGDPDGYGYRVHVPYQCCALRGDVTHDGSLAVSDLTYLTAYLFRGG
                     PAPLCPPEADLVVDGAIKVSDLTFLVSYLFRGGPAPTPCP"
     gene            complement(2866..4041)
                     /locus_tag="PKM94_04985"
     CDS             complement(2866..4041)
                     /locus_tag="PKM94_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD66029.1"
                     /translation="MRPLVRFVGAAVVLAIGLAADPAAGQARKPQNIHDNIADATNLD
                     CTQCHDCAEPRPGSSCLKPCPSHTMTHMTAKHQLSEAPETLLLDRLAAQYRPVTFNHK
                     RHASMAEMGNDCATCHHFSPPGRIPPCAECHGEESTAANLRQPSLKGAYHRQCLSCHR
                     EWSHDTKCVVCHLPADLAGVPADRVDSTDIMGVPHPIITVPEKRVYQTPYSEGPVVTF
                     YHDEHIDLFDLACANCHKKENCSYCHDLEQPARFAKSQEEVHGICNDCHRDDPCRRCH
                     DTSERPGFSHQATGFTLSGHHRDLNCRACHPTGKRISRISRECIACHAGWKAGTFSHT
                     ITGLQLDATHADFDCEACHLDFAYDEKPDCSTCHDDNRTHREAPPGVYVKPARVQKP"
     gene            complement(4041..5303)
                     /gene="hybB"
                     /locus_tag="PKM94_04990"
     CDS             complement(4041..5303)
                     /gene="hybB"
                     /locus_tag="PKM94_04990"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HOD66030.1"
                     /translation="MTAAFPVPIRTRFFTPGTKVLFGLMVAGLAAYAYRLIAGIGAAT
                     NLNDQYPWGIWIAIDVASGVALAAGGFTTAALADIFHREEYHVITRPALLTAMLGYTF
                     VVIGLLADLGRYYNVWHPMLPEMWQGNSVLFEVGMCVMIYLSVLYIEFLPIVVERFKG
                     RVRLPGPFRVLNRVTESWLELAEKYLPRFIFFFIIAGVVLSCLHQSSLGTLMVIAPTK
                     LHPLWYTPISPLLFLLSAISVGFPMVIFESILASRSFKLPPETQVLSSIARYTPIILG
                     VYLALKIGDITLRDAWPYVWAFSNESFFWLAEILFGVVAPIAILAGEKRRASVRWLFL
                     GASLVVGGVVLNRLNVFLVGYEPLYPQGTYFPSIFEIMVTVGLISALVLVYRWIVITF
                     PVISVPLHAVPEHARAAEGARPNLKGAK"
     gene            complement(5300..6214)
                     /locus_tag="PKM94_04995"
     CDS             complement(5300..6214)
                     /locus_tag="PKM94_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD66031.1"
                     /translation="MSMNRRAFLGVAATGSAALLLGRQPASADTIESADFSNSRGVLV
                     DTVACIGCRKCEAACNEANRLTSRSAETFEDKTVLEQHRRPDARAFTVINRFSDPARP
                     AEPYTMKVQCMHCNRPACVSACIVGALRKTPEGPVIYDAWKCIGCRYCMVACPFQIPA
                     YEYEKALDPQVRKCTFCYERITAEGKLPACVEICPNEALTYGRRDELIALARTRIKAY
                     PDRYVNHVYGEFEAGGTSWLYLSPTDFASTELPRLSHDPIPERTEKIQHGIFKSFVPP
                     LALYGLLGLIMHALRNGRSSQADREVKS"
     gene            complement(6733..8187)
                     /locus_tag="PKM94_05000"
     CDS             complement(6733..8187)
                     /locus_tag="PKM94_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD66032.1"
                     /translation="MRARLITALIFGMVLGGGPASEAATFRVPADYHTIQGAIDAADG
                     EDTVLVSDGVWTGPGNYDLVVPGTTIVIASVHGPAVTIIDCAGADFLDHRAFDLGSGG
                     LGAGRDNTFALRGFTIRNGVAAGGGGALVGDRWRLDIEYCIFESNRAVDGGAVSCDSC
                     RVDIRTSRFRYNLAERGGGALYGVGGGVISGCRFDLNRAETGGALADFRDLTFLDCVF
                     QQNEARYGAALRPLPADSVSFAASGTTFSRNRGDAAVFIAYGAADLSHCTFAENDGGL
                     ALTAAAPILTDCILADNAGKPVVCFDPMPGRTSDPFLSYCCVSDSGLDLPGCAVSFFT
                     MPNNLIADPMFCYLTPDDYRLEAGSPCVGAASDGGNMGAAPTGCSATDVGSDPDPPLA
                     AAFRLDQNWPNPFNPSTAIGYTLPQSSHVRLVVYDVLGRVVRVLVDAHRPAGAHTAEW
                     DGLTSSGAPAASGVYVYRFTAGPLSASKKMLLLR"
     gene            complement(8322..10640)
                     /gene="pbpC"
                     /locus_tag="PKM94_05005"
     CDS             complement(8322..10640)
                     /gene="pbpC"
                     /locus_tag="PKM94_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOD66033.1"
                     /translation="MPFRAPRRKVKLAAASLALALALLAALDRFVFPLPVERLSRPHA
                     RFVYSRDRVLLASFTSRDSFWRAPVPYNGLSPRLIESVLAVEDRWFRRHPGVNPVALL
                     GAAWDNLRAGRVVRGGSTITMQIARMMEPKERTVGGKVIEMLRAVQLEEHYCKDELLE
                     IYFNLVPYGGNIEGVGAAAHFYFDKPADRLTWSEAAILTAIPASPNTYRPDLHPEQCR
                     ARRDLILRRLHARGVIDAAELARVLTEEIPPGRQERPFVAPHFCQMLATACPDSAVLR
                     STLDFDTQLLCERLAARFHGFLAPRGIHNLAIVVLDNTDGSVLALVGSPDFTDRAHHG
                     QVNGAVARRSPGSALKPFVYALGFDDGLITPRSLLDDIPVAYAGYSPESYDEQYHGVV
                     SASNALVNSFNVPAVNLAYQVGLRRVHALLNDGGLGLNRKYYEYGLPLVLGACEVSLL
                     DLAALYSSFARGGRLLPPSLLAADSAAAGKPLVSPEAAWLTTTVLADLQRPDFPASWE
                     FTRDRPAVAWKTGTSYGRKDAWAIGYNPCYTVGVWTGNFSGAGSPYLVGAETAAPLMF
                     EIFDALMRGREPTWFARPAGIELREVCAVSGMPASADCPATVTDFAIRDRSPVRTCTL
                     HRRVLVDHATGHALCRACAVGRAVDTVTFEEWPARVTGWLLTTSAARPIPDHNPQCTG
                     LIQTDHPVIASPESDRVYELRPHVPRQYQKIPFRASIAHTGRRIHWFLDHELFATSAP
                     GDDVFYDPEPGPHTLMVVDDFGRSSTVSFVVQ"
     gene            complement(10652..16135)
                     /locus_tag="PKM94_05010"
     CDS             complement(10652..16135)
                     /locus_tag="PKM94_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HOD66034.1"
                     /translation="MRALWNRLSGRVPLVLLSLVAVIALATTLTLWAQLQTDDRNVRV
                     KDFRPQETTDRRTNVTITFSADLVSPDSLNRPVAAPPVSFEPPLAGAARWIEPAVLRF
                     FPESPLAPATAYTATVASDLAWRDGRRIAEKRTFSFRTDPLSIMGVSSYTMPAEDRPG
                     HVRVHVDLRFNYPIFIEDLTERLTIEGKKNAEAGRLSFSLLDVINTQTAGRSGAGDES
                     GGPATADIISLATDPVPASDSEQVYLVTIRAGVDCPNCGRPLERDHTGDLVVPPRERL
                     VVHEVGTYTEGIRPLVYLSFSTMIATDRVEDFVAVEPAVDFRAETRFNQILLTGAFTP
                     GETYTVSVASGLRSQAGAVLADDFSGRVTIQDIPPTVLFRSEGIYLPRENSGLLEIET
                     INVDSLAVEIEHIFANNLVYALAGGQADPFERGAADIRAVGRTFFTARHAVPERKNEK
                     VPTTVDVAGIVGDTARGIYKISAYSPTSRWVRDSRLALLTDLGLIARLSEDYLVVWVN
                     RLSTVEPVRGAEVRLYSRNNQLLLQGRTDSRGAAVFEKIVEQTAGFEPYVITVEYEGD
                     LSYLRFAETGLDLTDFEVKGRPLLTRGYEAFVYTDRGVYRPGETTHVAVLVRAPGGAV
                     PADFPYFLNIKDPTGRDFQTVRLTTGRSAFAATDLALPEYARTGKYTAIARIGDDLEI
                     GRTDFQVEEFVPDRIKVSVETARAAYTVGENIPINVAAEFLFGSPAANHAVAGRVQIE
                     PHTFVPSGYSKYSFADSQRKFMPRTDNLPDAALDGDGRHTYSYTVPRDLTPPSALRAL
                     IGAFVSEQGGRAVSGYREVIINPYPRYVGVRLDLEGYATPGEPFAARVIALNPEGRPV
                     PLAAAEARLYRVTWNTVLTRDSQGRYRYKSERDETLLQTQTVALADSGAVVSFTPDTH
                     GEFRLVIADPSGGHSADVSLYASGWGYAPWSMEEPERIEIGLDKTVYRPGEDAVVQIR
                     APFGGTLLVTVEKETVHESFVMAMPDNTAEVRLRVKDGYFPNAYVTATVLKKAAEVDR
                     SAPARAFGIAPLFLQKSERDLNIALEAPEVMQPHRTMTVGVRVGVPGPTQLTLAVVDA
                     GILQLTGFAAPDPLEFFYGQRAPSLRPYDIYSFLYPNIQAAESHLAPGGDRFFEAARR
                     RHLNPFAAGRVQPVALWSGLVTTDASGRASIDFRLPAFNGRLVLMAVAAQGNRFGSAV
                     REVTVRENLIVQESFPRFVSPNDVVTGLVTVFNHTGDSGEVTVELELDGPAEAVGDKA
                     RTIALADGAEGSVAFTFKAGVVPGPNAVRIRATAGADQTEAAFQLPNRPARPLSTRYG
                     AGVVTDTAAARIVPPADVIPRTARFTLQTSSLNALQFRRQIEYLLSYPYGCVEQTTSR
                     VFPLLYFESLARFVSPELLGGNAPDYFVQEGIRRLGAMMRADGTFSFWPGGGPPSPWT
                     SVYAGHFLLEARQAGFVVEDDLYEAVRKRLLAWARGRDLTDADAEIRVYAAYALASTD
                     QLDRKILNELRQIPIAPLSLQAKFHLAGALALAGDRTAAFAIIDPIEIQPAVFPPETG
                     GTLASGTRTSAVMLDVLARLDPDNPSAAVLARDLTESARVGRWYTTQESAFALMALGR
                     YLGSRPAADFTGRVALAGDSAYTFDETGLSLTRENFLGREVTISIDGAGPCYYYWQLS
                     GVSTSPAVEEFARGIRVAREYLDADGNPLELDSVRLGDQVICRIEAIAERDNLSHVAI
                     NDLLPAGFAVENPRLKTTPRLAWIPRESAAIGNSDIRDDRVLLFVDLNPKTAVVYHYS
                     LRAVAAGEFQVPPIAAECMYNPVIAGASGSGVLTVRRPGGDGR"
     gene            complement(16271..17098)
                     /locus_tag="PKM94_05015"
     CDS             complement(16271..17098)
                     /locus_tag="PKM94_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOD66035.1"
                     /translation="MKPVYHFVRLLGTRAGWPDDMTPREEQVMANHFAYLKDLTRKGI
                     VVAAGPVFNPTFGLIILRTGSEAEARALMDSEPSVAAGVHTYELQPMTLSLLADHRSP
                     LRYVSRPSARSLRKEIVVPVSADSAWTLWTTNAGLEAFLVEKANVDLRLGGSFELYFS
                     MASPVGQRGSEDCAILSFLPGRMLSFEWNAPPSFPSRRDGERTWVVVEFEPLAPDRPA
                     ADSGVAPAAPAAAQTRITLTHLGWGEGEDWDSLYAYFDRAWGLVLDALREYLAGTAE"
     gene            17561..20209
                     /locus_tag="PKM94_05020"
     CDS             17561..20209
                     /locus_tag="PKM94_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD66036.1"
                     /translation="MRTRFFGCVLLGCLLLGGAALAQEDPGIPDTMKLVATVCPEAAT
                     GQLKVVVELWGWTDHLVMGNGAGFTWDHPSLVMDSAKPSAALDDAYDVGPFFYENDNL
                     ALTNANRRFLTSGAVMFSSGLPGSTQWRHWATYYFTLTGWSPSDVITLDTLSFSPGSA
                     WDMTAVNDVGQDVYFEPRWKGALHIGDNCEYCVDVDNDGFGDPTVPGQTCLPDNCPTV
                     SNPTQADADGDGLGDACDNCPTMANQNQADGDGDGKGDVCDNCPAIANPDQLDSDGDG
                     KGNVCDNCPTVANPTQADGDGDGFGDACDNCPSLANPDQADADGDGDGDVCDNCLLVA
                     NPTQADADGDGLGDACDNCPAVNNPGQEDGDGDGDGDACDNCLEIANPDQADSDLDGR
                     GNACDNCPTVANADQADGDGDGRGDVCDNCPTIANADQMDSDGDGLGDACDVCPNDPD
                     NDVDGDNVCGDVDNCPAVANADQADGDGDSIGDVCDNCPTDSNTDQADGDADAIGDVC
                     DNCPAEANVDQADGDEDTVGDACDNCPAVVNGDQADGDADAIGDVCDNCPAEANTDQA
                     DLDDDGLGDACDNCPAIANADQADGDGDNVGDVCDNCPAEANTNQADLDDDGLGDACD
                     NCPAVANADQADGDSDGVGDACDNCPAIANADQLDSDGDGIGDACDECPFDAENDIDG
                     DNVCGDVDNCPAVANADQADADGDNVGDVCDNCPAAANPDQDNADGDLYGDACDNCAA
                     EPNDDQADADGDNVGDVCDNCPATANVDQTDGDGDGVGDACDNCPEKYNPDQADTDQD
                     GIGDVCDGCCVGMRGDVNGDGEPVANISDVTYLVNYLFRHGPEPPCLAEANINGDGLE
                     KVDVADLTHLIAYLYRMGPAPANCPW"
     gene            20398..21033
                     /locus_tag="PKM94_05025"
     CDS             20398..21033
                     /locus_tag="PKM94_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66037.1"
                     /translation="MGVRAGVAAALAALVIGCSSGDKQAGTAGGPSGMADRIARVDPA
                     QFDLTGRPVALAGMTFQPPAAWVDLGPSGMRQASYYLRPVSGDADSATVTVFYFGPGG
                     GGSVAANIDRWIGQMATGEKDNPSERAERSSLQADSLPVHVISVGGTYSGAMGGPMGG
                     GGEAHADYRMVAAVIEAPEGNVFFKLTGPEKTAVEMQSGFFAMLKSVKRAG"
     gene            21425..21988
                     /locus_tag="PKM94_05030"
     CDS             21425..21988
                     /locus_tag="PKM94_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66038.1"
                     /translation="MQDNDAQVIEQALAGNQQAYRILTERHRPSVFHIVYKIVRDRET
                     ANDLVQETFMKAFSSLASYRSEYRFSTWLYKIAANCAIDHLRKRRIQVLSLEGQMDRD
                     SSGRTIEVPDYSYHPEHDLVRKEQRFNIEEAIDSLPKKYREVIVYRHKEDKSYEEIAD
                     LLGIPIGTVKARIFRARELLKKKLKSI"
     gene            22015..22926
                     /locus_tag="PKM94_05035"
     CDS             22015..22926
                     /locus_tag="PKM94_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOD66039.1"
                     /translation="MPRLSRTVARLLILLAATAPPAAGEEFTFDYQKIVEAPEPVRLD
                     LRLVRGSVEITGAEDNRVVIEAVKRVRAGSRDEAEELADHIEIKTRLNGTAVEVQTNY
                     LGITSRGRSFWQKVFGKGEDAPGEVAYKVSVPVRSDVTVQGLAATITLASIEGAVRVE
                     NESGATRAEYIFGPVTVSQPQGDIDLLWIEGDIRVKATTGTVTVNQVRGALDIATHTG
                     EVHVRTELDSPRDFFVATTSGKITFAIPPAASGRFLVETASGGIQSQVPLVIESVTRQ
                     QLIGSFGRGGPRINLTTSSGDVTVAQY"
     gene            22959..24257
                     /locus_tag="PKM94_05040"
     CDS             22959..24257
                     /locus_tag="PKM94_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD66040.1"
                     /translation="MRTAVRKFGLSLLLAGLLAGPRQFPAVLAQEAPDERAAESSLAA
                     DLPADVAGPATAPPGIVFFEIRLSEDRAVATDTSGREWVYDFNADVWVEGTPGQEGGR
                     LTDRLQPRTTLPIDLRAIRLVAVKPFERSVTIGYDEYVDGDIIATGRVTVKGWVKGDV
                     RSIDKRVLVTETGQVDGSVEAPSVIIRPGGVVSGTVRETGGPLDVESLTRAVSVDALV
                     LATALACGLLFFGFLVLAVAPRQAGRIDACITGHRVRTCALGFLFSLLLPVIIALFII
                     TLVGILVVPFVPLVYAGAIGMGFVSFGGTIGGWVLRRLTGARQGTQLRTALGTLLLAL
                     LWLLVIVLLGGGNAFLLGLGYALLAAAIPVTSFPVFAGIGGAILTRFGFRDYVSWKER
                     KPADTGPPAPAPPPLHEPPPIITPWPPAPEPPERREPPQE"
     gene            24340..25191
                     /locus_tag="PKM94_05045"
     CDS             24340..25191
                     /locus_tag="PKM94_05045"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HOD66041.1"
                     /translation="MRGVFRFWLLAALAALPAAALQARERKNTPVAAEGAERINVQID
                     FAAGKLRIDPGEIAEAAVFDFAYDEDKVDCAVDYEVRGGVGMLQAESERLSGSGGDTG
                     DNEWEVVFSRAYPMNLSLDIGACDAEIELGGLRLEEVAVEFGAASAEIGFREPNPARM
                     RELRFEAGASSVTATDLGNANFESCEFEGGVGSFDLDFRGEYHGESEIRVKVGLGSAE
                     ITLPVDIPCRIEGPGGGLLSSVEIHGGGLREIADDVWESDDYAGASTRLRLILELGLG
                     AVDVRFR"
     gene            25452..26834
                     /locus_tag="PKM94_05050"
     CDS             25452..26834
                     /locus_tag="PKM94_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOD66042.1"
                     /translation="MHLRQVGVVLLFLFMCAAVTRAAAPTDLQERIYYARDRVLPALV
                     HIQPVIRNYDTGELEKQAVIGSGVIFHKDGYVVTNYHVAGKAVRIICTLYDKEQVPAE
                     FVGGDPMTDLSVIKLDLSKYDKPLQPAEFGDSDSIHVGQYVLAMGSPLSLSRSVSAGV
                     ISTRDRYFDDDVRLPTGEQTGKYNLWIQTDAAINPGNSGGPLVDLDGRVVGINSRATL
                     FANNIGFAIPVNVVKEVTAAILDHGAVERSWIGVHAQALQELADFFGADSGEGVLISS
                     IDPGSPAEARFLKAGDIITAVDGRPVSARFVEELPAFYNRISRIPPGSEVTLSVKRDG
                     ATMPVRLRTKRLGDLQGEDFEAKGWGFTVKAITRQMQIDATLPDTLGVIVVGVKRTGP
                     AGEGGLRAGDVIQKVNDAAVGSLEEFAARYRELSALDTERILLTVSRTGSTRFVLLKA
                     DKNQEVTPHE"
     gene            26827..28281
                     /locus_tag="PKM94_05055"
     CDS             26827..28281
                     /locus_tag="PKM94_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD66043.1"
                     /translation="MSKRGHGRMVPRLAPAAVWAALWLLAPAAAFAQHFDFRTLEEKV
                     RAFTVVIDMDVEISFGVHSTEQTDRYLGTIVNDSGLVIFDGSGVGTETAIPSFSGFAV
                     KTRPVRITVTSMDGKTYAGEYLGYDRYTNFAFLQIDGKGDTFTPVAFRGGQEFQVGQW
                     VGLFWLLPEFVSPPLAADVGMVAARVTSPEEMALTVGFSSLQAASVLFSEQLEPVGML
                     GALADPTTGGDDGSGMLGTFGDAGIPLLGVVTAERLTRLIAEPPRQGEGGRGWLGITL
                     QALTADIAAFWNLETLGGIIVNEVVKGSPAEEAGLEVGDVIFEINGQAIDVDKEEKLP
                     LFQRTISDLGPGAAVELGVLRLGPSTIDTLHVLVTLADAPIAAADAKELKVDEFEFTV
                     RDLVFSDYMIANQDPEKFEGVVVSKIEAGGLAELSGLQIGDVIQRLGSTPVRSVADVE
                     SVMTGMKVEKPREVILFVWRGGKTMFVNLKTDWP"
     gene            28653..29510
                     /locus_tag="PKM94_05060"
     CDS             28653..29510
                     /locus_tag="PKM94_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD66044.1"
                     /translation="MRKLLLTVLAVLLCAAGSEAVPPVAPWAAQVTLDTVAARPGQHV
                     AVPIRLSNNDQVSTGIYIPLRLASSSLTVDSVSFVGSIMTADFTGMAHVSASGLTTVE
                     IALAPNLVAPIPTIPAVNGIIARLWVTVSPSAPLGLARIDSLAIDSALSDGGPAARLW
                     KYVQGATAEGVTMMPGFRAGGVLVESPTGIEDEENLMPGRFELAQNYPNPFNPSTTIG
                     FTLPTAQDVQLVVYNVLGQEIATLVNGRMAAGSHQVTFEASGRPSGVYFYRLTYEDGV
                     ETRKMTLVK"
     gene            29592..31304
                     /locus_tag="PKM94_05065"
     CDS             29592..31304
                     /locus_tag="PKM94_05065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD66045.1"
                     /translation="MTQAVDNPAPPKAVRIGPYLLTAKIGQGGIAVIYKGRQESLDRD
                     VAIKILSPNLSTDPDIVRRFERESMVIARLNHPNIVHIIDKGEAGGRYYFVMDYIDGT
                     SLREVIDSAKIPLRTKLEMIVQVCKALDYAHKNGVIHRDIKPANILIDRQGNALVADF
                     GIAQIVGTPEAEMTASDVVMGTLSYMSPEQKVSSAGVDHTTDIYAVGVILYEILCGKK
                     PLGKFKLPSEITADLGEAYDGIISKCLAQDRGERYQAAVELKDALLEAMGGGSLRGDT
                     SDLTVSGTESFLGKCRYLDTIRETQFGSTILVENRVNKRLYVIKKHHKGEAGRKEAKL
                     LATLKHEHIVDIYGAGGDRKSTVIIAEYMQGGSLADRMVRKYDWEKALAIIRQVAAAV
                     DFAHKNNILHGNLRPSNILFDKDDVVKLSDFALPIHYDGPKQKNWYSPPERKSSRQGD
                     VYGIGVILHQMLTGRNPRYDENDNLDLDDIRMFLPEEIAAMLDRLLAIRIARRYRSCA
                     EFLLDWEDFDRRRQEERRQQESRAAAAPAAVPGKPVWLYLAIGAGIAFALFTVLYLAG
                     VFSL"
     gene            31451..>32087
                     /locus_tag="PKM94_05070"
     CDS             31451..>32087
                     /locus_tag="PKM94_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66046.1"
                     /translation="MRREFIGVGIIYALATGLVLLASTVRPRGLWAFSAASYFGDPWA
                     WAPAAAAVLWAAAVLLYGRRAGDGGDASDGGRRFAWWAVSHTALMAALFIGLRARTHF
                     LGDGYTLLGTYATENPLIKSRALGEALVHLWVRDLVGGPTKAAALLSFQVLAVGAGLI
                     YLGLVWWAAARLFEHRRERLLFAVGMTTPGYMLLYCGYVELYALFVTAVAAF"
BASE COUNT         5688 a         9827 c        11476 g         5096 t
ORIGIN      
        1 aagggcagtt cattcagcgg cagatcgtca tcagccagcc ggatctgcag ggcctctaca
       61 tcttcgacct ggtagtaccg gactacaacc ccgagtgggt ctcggtggat gtgcaggggt
      121 tcaatttcca gatgctcgag atggagccga acttcatcat ccacacctgc aagggatcgc
      181 tcgacctgtc gtttgttatc accggcgggg cggcgtcggc ggacacctgc gactactaca
      241 agccgcggca gctcgactac gcgccggagg ggatgccgga cttcgacatg aagcaggcgg
      301 gctggaccga tcagctcggg aggtggtcgc atgacggccc ggcggcgctg gccaactgcc
      361 tgtggtggtt cgattcgaag ttcgagccca acccggtgga tccccggccg ttcggcatgg
      421 tgccgcccaa cgacgggtat cccctggtga cggcctacct tccgaccgac gaccacgatc
      481 cgaccaacgt ccagccgttc atcaccgacc tggcggccaa ctacgtgcag acgaatgtcg
      541 gcgggaccgg gacacagccc ggatacctgc agaccggatt caagaactat ctggccgtgc
      601 gcggcctcgc gggtcagtac cgggacaccg cggtgctggc gccgactttc gaatatatcc
      661 agcggcaggt tctcgactgc caggatgtca ttctgctgct gagtttctgg gaggaccgcg
      721 gcgccgccgc cgccagctat ataggcagcc actatgtgac ggtggccggg gtgtgcaccg
      781 atttggccca gcggcagatg tgtatttcgg atccgtggct ggatgcgctc gaaggcgagc
      841 cgccggcggg cagcgcgcac atcgggaccg tgcacaacga cgccgacaat atcagcgggc
      901 cgcacagcca gattcagcac gacccgtacc aatgtatgag cgcgaccccg cccgggttca
      961 cggggccggt cgaggtggtg aactacccgg ccgatgccat gacgctgaac aatttctacg
     1021 gcatgaacct gaatatcgac ccgacgccct acggcggggg ccaggtgtat acggtcatca
     1081 ccgaggcgct cgtaatctgt ccgcatacgg tgcagccgat cgacacctgc gagtactaca
     1141 agctccccta cgaggactat gcgccctatg gaatgccgga cttcgatcag aagcaggaca
     1201 actggcggta tccgccgtcg ggagcctgga gccactgcgg accggtcgcg ctggccaatt
     1261 gcttctggtg gttcgactcg aaattcgagc cgagcccggt cgatccgcgg ccgtttgcgc
     1321 caagcccgat ctcgccgccg tggaacgaca actacccgct ggtgggcatt tacccggtgg
     1381 cgggtgcatg ggacgaccac gacacgaaca acgtgaagcc gttcgttgat tcgctggcgc
     1441 tgtatgcgca gacgaacgtc ggcagcagcg ggaccagcgt gctcaacctg gcgaccgggg
     1501 cgcagaactg gttggcgagt gtcgggttgg gtacgcgcta taccatggtg gtgatgccgc
     1561 tcgatcccgc ggtgccctat gaattcatcc gcgaccaggt gctcgagagc caggacgtga
     1621 tcctgctcat cgggttctgg caggagatgc agacggggta ctgcgagcgc atcggcgggc
     1681 actatgtgac ggtggcgggg acgtgcccgg atccgcttga ctcggcgttc tgcatttcgg
     1741 atccgtactt cgacaacaac gagggcgaac cgccggccgg gtcggcgcac agcgccggcg
     1801 cgcacaacga cgcggccaac gtctccggtc cgcacgggac gatccaccac gaccgctacg
     1861 acgtcatgcc ggcggcctgt cagcagtttg tcggggcgcc tctgttcacg atggagttgg
     1921 cgggttatcc gacggcgacg gccgacatcg ccccgttcta cgggcagaat ccgttcgatc
     1981 cctcgctgcc gccggtgccg ccgcagccgg ggctgcccgt ccacgcgatc ctcgagtggg
     2041 cgatcattat ttgcccgcag ccgccctccg gggccgacac cgtgaagtgc gagccgcagg
     2101 gcgggaccaa cccgaaccat cccaactcgt actggtacga cgtgaagccg gggaatccga
     2161 ccgggctgtt cgacttccac gtgaaggtcc tggacacgat cccgcgctac tacctgaact
     2221 ggctcgagcc ggccgggtgg ctgcaccagc tccacaaggt cggcgccgac tggtgggtct
     2281 cgtggtggga tccgacgggc ggcaacccga tcacggccac attccggttc ggcttcgaca
     2341 actggaatcc ctccgcctgg aacgactggg tgacaacgac gagcgcttcg aacaacccgg
     2401 ccgcgggcgt gagcgacaac tccggcaacc acgtgggcga tccggacggc tacgggtacc
     2461 gggtccatgt cccgtaccag tgctgtgcgc tgcggggcga cgtgacgcac gacggatcgt
     2521 tggcggtcag cgatctgact tacctcaccg cctacctgtt ccggggcggc ccggcgccgc
     2581 tctgtccgcc ggaggccgat ctggtggttg acggcgcgat caaggtgagc gatttgacct
     2641 tcctggtcag ctacctcttc cggggcggtc cggcgccgac gccgtgcccg taacgccttc
     2701 gaccgacgag ccactacggt ctgttattaa gaagcgcctg ctccctgacg ggagcgggcg
     2761 cttcgctcag gcgggacgcg gacggacatc cgcgcagggg caaaagaaca cggccctccc
     2821 gatcgggagg gccgtcgtct atccgtcgcg ctctgctcaa cggccttacg gcttctgcac
     2881 ccgggccggc ttgacataga cgcccggcgg cgcctcccgg tgcgtccggt tgtcgtcgtg
     2941 gcaggtggag cagtccggct tttcatcgta ggcgaaatca aggtggcacg cctcgcagtc
     3001 gaagtcggcg tgggttgcat cgagctgcag cccggtgatc gtgtggctga aggtccccgc
     3061 tttccagccg gcgtggcagg cgatgcactc gcggctgatg cggctgatgc gtttcccggt
     3121 cgggtggcag gcgcggcagt tcaggtcgcg gtggtggccg ctgagcgtga aaccggtagc
     3181 ctggtggctg aaaccggggc gttcggaggt gtcgtggcag cgccggcagg ggtcgtcccg
     3241 gtggcagtcg ttgcagatcc cgtgaacctc ctcctgcgat ttcgcgaagc gcgcgggctg
     3301 ctccagatcg tggcagtacg agcagttttc ctttttgtgg cagttggcgc aggcgaggtc
     3361 gaacagatca atatgctcgt cgtggtagaa cgtgaccacg gggccctcgc tgtaaggcgt
     3421 ctggtagacg cgcttctcgg gcacggtgat gatagggtgg gggacgccca tgatgtcggt
     3481 ggagtcgacg cggtcggcgg gtacccccgc caggtccgcg ggcaggtggc aaacgacgca
     3541 cttggtgtcg tggctccatt cgcggtgaca ggagaggcac tggcggtggt aagctccctt
     3601 gaggctgggc tggcggaggt tggcggcggt actctcctcg ccgtggcatt ccgcgcaggg
     3661 ggggatgcgc ccgggcgggc tgaagtggtg gcaggtggcg cagtcgttcc ccatctccgc
     3721 catggacgca tggcgcttgt ggttgaacgt gaccgggcga tactgcgcgg ccagccggtc
     3781 cagcagcagc gtctcagggg cttcggacaa ctggtgcttg gccgtcatgt gagtcatggt
     3841 gtgcgagggg cagggcttca ggcaggagct gcccgggcgc ggctcggcgc aatcatggca
     3901 ctgggtgcag tcgaggttgg tggcatcggc aatgttgtcg tggatgttct gcggctttcg
     3961 cgcctgaccg gcagccgggt cggccgcaag cccgatcgcg agcacgaccg ccgcaccgac
     4021 gaagcgcacg agaggtctca tcacttcgct cccttcaggt tgggtcgggc gccctcggcg
     4081 gcgcgcgcgt gctccggcac ggcgtggagc ggaacggaga tgacggggaa ggtgatgacg
     4141 atccagcggt agacgagcac gagcgcggag atgaggccga cggtgaccat gatttcgaaa
     4201 atcgacggga agtacgtccc ctgcgggtag agcggctcgt agccgacgag gaagacgttc
     4261 aggcggttga ggacgacgcc gccgacgacc agcgaggccc cgaggaagag ccagcggacc
     4321 gaggcccggc gcttctcgcc ggcgagaatg gcgatcgggg cgaccacgcc gaagaggatc
     4381 tcggcgagcc agaagaacga ttcgttggaa aacgcccaga cgtacggcca ggcgtcgcgc
     4441 agcgtgatgt cgccgatctt cagggcgagg tacacgccga ggatgatcgg ggtatagcgg
     4501 gcgatcgacg acaggacttg cgtctcgggc gggagcttga acgagcggct ggcgaggatc
     4561 gactcgaaaa tgaccatggg gaacccgacc gagatggccg agagcaaaaa cagcagcggc
     4621 gaaatcgggg tgtaccacag cgggtgaagc ttggtcggcg cgattaccat gagggtgccg
     4681 agcgacgact ggtggaggca ggagaggacg accccggcga tgatgaagaa gaagatgaag
     4741 cgggggaggt atttctcggc cagttcaagc caggattcgg tgacccggtt gagcacgcgg
     4801 aacgggcccg gcaggcgcac gcgccccttg aagcgttcga cgacgatggg gaggaattcg
     4861 atgtagagga cgctcaggta gatcatcacg cacatgccga cctcgaacag aaccgaattc
     4921 ccctgccaca tctcggggag catggggtgc cagacgttgt agtagcggcc gaggtcggcg
     4981 agcagcccga tgacgacgaa ggtgtagccg agcatggcgg tcagcagggc cgggcgggtg
     5041 atgacgtggt actcctcgcg gtggaagatg tcggccaggg cggcggtggt gaagccgccg
     5101 gcggccagcg ccaccccgga ggcgacgtcg atggcgatcc agatgcccca gggatactgg
     5161 tcgttgaggt tggtggccgc gccgatgccg gcgatcagac ggtaggcgta ggcggccaga
     5221 cccgcgacca tcagcccgaa gaggactttg gtgccggggg tgaagaaccg ggtccggatc
     5281 gggacgggaa aggcggcggt catgacttca cctcccggtc ggcctggctg ctccggccat
     5341 tgcgtagcgc gtgcatgatg agcccgagca ggccgtagag cgcgagcggc gggacgaacg
     5401 acttgaagat gccgtgctgg atcttctcgg tgcgctccgg gatcgggtca tgggacagcc
     5461 ggggaagctc ggtggaggcg aagtcggtcg gcgacaggta gagccaggag gtgccgccgg
     5521 cctcgaactc gccgtagacg tggttgacat agcggtcggg ataggccttg atccgggtac
     5581 gggcgagggc gatcagctcg tcgcgccggc cgtaggtgag ggcctcgttc gggcagatct
     5641 ccacgcaagc cggcagcttc ccctcggccg tgatgcgctc gtagcagaac gtgcacttgc
     5701 gaacctgagg atcgagagcc ttctcatact cgtaggcagg gatctggaag gggcaggcca
     5761 ccatgcagta gcggcagccg atgcatttcc aggcgtcgta aatgaccggg ccttccggcg
     5821 tcttccggag agcgccgact atgcaggccg agacgcaggc gggacgattg cagtgcatgc
     5881 actgcacttt catcgtgtac ggttcggccg gccgggcagg atcggagaag cggttgatga
     5941 cggtgaaggc gcgggcgtcg ggacggcggt gctgttcgag caccgtttta tcttcaaatg
     6001 tctccgcgct ccgcgaggtc agccggttcg cctcattgca cgcggcctcg cacttgcggc
     6061 agccgatgca ggcgaccgta tcgacgagca cgccgcggct gttggagaaa tcggcggatt
     6121 cgatcgtgtc ggcggaggca ggttgtcggc cgaggagcag ggcggccgag ccggtggcgg
     6181 cgacgccgag aaaggcgcgt ctgttcatgg acatgatgtc atccttcggt tgtcggcagc
     6241 ctccaggccg cgggaccggt tatgtgaaat atttcacatc gtcttgggca cgttaacatt
     6301 gtggagaaat actaccatca tatctctctt ttgtcaagaa gtatcggtag gaatgctgaa
     6361 gaaaataaca gggagaaaga ttcaatcagc agagagattt attaagtatc tcacacataa
     6421 tgaaatacgg ctacacgccg gtgaagatgc atcagctgcg ccttcggact taatctacct
     6481 cgtcaacctt taatatcctg ttggaggctc ctcccccgcc gacggacccc acttgtcggg
     6541 aattccgggg tactcaagga aaatcggcca aaactccaat tgccccggtc gacgtcgttg
     6601 tcaaagcggg tcaggcccgg aggctgcggc acaatgcggc aggcacggtc cagggtcggg
     6661 agcgaagcgg ccggcggcca gaccgcaggg ggaagggccg ggtcaacggc agggagaggg
     6721 cagaccgtca ggctatctca ggaggagcat cttcttcgat gccgacagtg gcccggcggt
     6781 gaaccggtag acgtagacac cgctggcggc cggtgcgccg gaggacgtca gaccgtccca
     6841 ctccgccgtg tgggcgccgg ccggccggtg ggcatcgacg agcacccgaa ccacccgccc
     6901 gaggacgtca taaacgacga gccgcacgtg cgaggactgg ggaagggtgt agccgatggc
     6961 ggtcgacgga ttgaacggat tcggccagtt ctgatcgagg cggaatgcgg cggcgagagg
     7021 gggatcggga tcgctgccca cgtcggtggc ggagcagccg gtcggggccg cgcccatgtt
     7081 gccgccgtcg gaggcggccc cgacgcacgg ggatcccgcc tcgagccggt agtcatcggg
     7141 agtgaggtag cagaacatcg ggtcggcgat gaggttgttc ggcatggtga agaaggacac
     7201 cgcgcacccg gggagatcca ggcccgaatc cgaaacgcag cagtaactga ggaaggggtc
     7261 gctcgtgcgg ccgggcatcg ggtcgaaaca aacaaccggt ttcccggcgt tgtcggccag
     7321 gatgcagtcg gtcaggatcg gagcggcggc ggtgagcgcc aggcccccgt cgttctcggc
     7381 gaaagtgcag tgggagaggt cggccgcgcc gtaggcgatg aaaacagcgg cgtcgccgcg
     7441 gttgcggctg aaggtggttc ccgaggccgc gaacgagacg ctgtcggccg gcaggggccg
     7501 cagggcggcg ccgtagcggg cctcattctg ctggaacaca cagtcgagga aagtaaggtc
     7561 gcggaaatcg gcgagcgcgc cgccggtctc ggcgcggttg agatcaaacc gacagccgct
     7621 gatgacgccc ccgcccacgc cgtacagcgc gcccccgccg cgctcggcga ggttgtagcg
     7681 gaagcgcgag gtgcggatat cgacccggca ggagtcgcaa gagacggcgc cgccgtcgac
     7741 ggcgcggttg ctttcgaaaa tgcagtattc gatgtcgagg cgccagcggt cgccgacgag
     7801 cgcgccgccg ccgccggccg cgaccccgtt gcggatcgtg aacccgcgca gcgcgaacgt
     7861 gttgtcgcgc cccgcgccga gcccgccgct cccgagatcg aacgcgcggt ggtcgagaaa
     7921 gtcggctccc gcgcaatcga tgatcgtgac cgccgggccg tggaccgatg cgatgacaat
     7981 cgtggtgccg gggacgacca gatcgtaatt gccgggaccg gtccagacgc cgtcggatac
     8041 cagcaccgta tcctcgccgt cggcggcgtc gatagcgccc tggatggtgt ggtagtcggc
     8101 ggggacgcgg aaagtggcgg cctcggatgc cgggccaccg cccagcacca ttccgaaaat
     8161 cagcgccgtt ataagccggg ctctcatagc gaaccttact gcctcacctc tcgtcggcgg
     8221 ataactcatc ctgctgtccg taatatatgt cggaatcgga acatgccaag ttcttcgttt
     8281 gttcgccggc cccggcgcag gatctgtcag gggccggcaa cttactggac aacaaaggag
     8341 acggtgctgg agcggccgaa gtcgtcgacc accatcaggg tgtgcgggcc cggctcaggg
     8401 tcataaaaga cgtcgtcgcc cggcgcgctg gtggcgaaca actcgtgatc gaggaaccag
     8461 tggatacggc ggccggtgtg ggcgatggag gcgcggaacg ggattttctg gtactgcctt
     8521 gggacgtgcg gccgaagttc atacacccgg tcggactccg gagaagcgat gaccgggtgg
     8581 tcggtctgga tgaggccggt gcactgcggg ttgtgatcgg ggatgggccg ggcggcgctg
     8641 gtggtcaaaa gccagccggt cacccgcgcg ggccattcct cgaaagtcac ggtgtcgacc
     8701 gcgcggccga ccgcgcaggc gcggcagagg gcgtgccccg tggcgtggtc cacgagcacc
     8761 cggcggtgca gcgtgcacgt ccgcaccggc gagcggtcac ggatggcgaa atcggttacg
     8821 gtcgccggac agtcggccga tgccggcatc ccggagacgg cgcagacctc gcgcagttcg
     8881 atcccggccg gccgggcgaa ccaggtcggt tcccggccgc gcatgagggc gtcgaaaatc
     8941 tcgaacatga gcggcgcggc ggtctcggcc ccgaccaggt agggcgaccc cgcgccggag
     9001 aaattgccgg tccagacgcc caccgtgtag cacgggttat aaccgatcgc ccaggcgtcc
     9061 ttccgcccgt acgacgtgcc ggttttccag gcgacagcgg ggcggtcgcg ggtgaattcc
     9121 cacgaggcgg ggaagtcggg ccgctggagg tcggcgagga cggtcgtcgt gagccacgcc
     9181 gcctcgggag agaccagcgg tttcccggct gcggctgaat cggccgcaag cagggaaggc
     9241 ggaagcagcc gcccgccccg cgcgaacgac gagtagaggg cggccaggtc cagcagcgac
     9301 acctcgcagg cgccgagaac gagcggcagg ccgtactcat agtacttccg gttcaaaccg
     9361 aggccgccgt cgttgagaag ggcgtggacg cgccggaggc ccacctggta ggcgaggttg
     9421 accgccggca cattgaacga attgacgagc gcgttgctcg ccgagaccac gccgtgatac
     9481 tgctcgtcat agctctcggg ggagtagccg gcgtaggcga ccgggatgtc gtcgagcaga
     9541 gaccggggcg tgatcaggcc gtcgtcgaag ccgagcgcgt agacgaacgg tttgagggcg
     9601 gagccggggg agcggcgggc gacggcgccg ttgacctggc cgtggtgggc ccggtcggtg
     9661 aaatcgggcg aaccgaccag ggccaggacg ctgccgtcgg tgttgtcgag caccacaatc
     9721 gcgagattgt ggatgccgcg gggagcgagg aaaccgtgga agcgggcggc gagccgctcg
     9781 cacagcagct gggtgtcgaa atcgagcgtg ctgcgcagca cggccgagtc cgggcaggcg
     9841 gtggcgagca tctgacagaa gtgcggggcg acgaaaggcc gctcctgccg cccggggggg
     9901 atttcttcgg tcagcacccg ggcaagttcg gccgcgtcga tcaccccccg cgcgtgcagc
     9961 cggcggagaa tcaaatcccg ccgcgcccgg cactgctcgg ggtggaggtc gggacggtag
    10021 gtgttgggcg aggccggaat ggccgtcagg atcgccgcct ccgaccaggt gaggcggtcg
    10081 gccggtttgt cgaagtagaa gtgggccgcg gcgccgactc cctcgatgtt gcccccgtac
    10141 ggtacgagat tgaagtagat ctcgaggagt tcgtctttgc agtagtgctc ctcgagctgg
    10201 acggcgcgga gcatctcgat gaccttcccg ccaaccgtcc gctctttcgg ctccatcatg
    10261 cgggcgatct gcatggtgat ggtggagccg ccgcggacaa cccgcccggc gcgcaggttg
    10321 tcccacgccg cccccagcag ggcgaccggg ttgacccccg ggtgccggcg aaaccagcgg
    10381 tcctcgaccg ccaaaacgct ctcgatcagg cgcggcgaga ggccgttgta cgggaccggc
    10441 gcgcgccaga acgaatcgcg cgaggtgaag ctcgccagga gaacacggtc gcggctgtaa
    10501 acgaaccggg cgtgcggccg ggacaggcgc tcgaccggga ggggaaagac gaaccggtcg
    10561 agcgcggcca gcagggcgag ggcgagcgcg agactcgccg ccgccagctt cacctttcgg
    10621 cgcggcgcac ggaagggcat cgggtcctcc gctacctccc gtctccgccc gggcggcgca
    10681 cggtgagcac gccgctgccg gaggcgcctg cgatcaccgg gttgtacatg cactcggcgg
    10741 cgatgggggg aacctggaat tcgccggcgg cgacagcgcg gagcgagtaa tgatagacaa
    10801 ccgcggtctt cgggttgagg tcgacaaaca gcaggacgcg gtcatcgcga atgtcggagt
    10861 tgccgatggc ggcgctctcg cgggggatcc aggcgaggcg cggggtcgtc ttcagacgcg
    10921 ggttctcgac ggcgaatccg gcggggagga gatcgttgat cgcgacgtgg gagagattgt
    10981 cccgctcggc gatcgcttcg atgcggcaga tgacctggtc gccgaggcgg acgctgtcga
    11041 gttcgagcgg gttgccgtcg gcatcgagat actcgcgggc cacccggatc ccgcgggcga
    11101 attcttcgac cgcgggagaa gtcgacacgc cggaaagctg ccagtagtag tagcacggac
    11161 cggcgccgtc gatgctgatc gtgacctcgc ggccgaggaa attctcgcgc gtgaggctca
    11221 ggccggtttc atcgaaggtg taggcggagt cgccggccag ggcgacgcgg ccggtgaaat
    11281 cagcggccgg gcgcgacccg aggtagcggc cgagcgccat gagcgcaaag gcgctctctt
    11341 gggtcgtgta ccaccgtccg actcgggcgc tctcggtcag atcgcgggcg agcacagccg
    11401 ccgaggggtt gtccgggtcg aggcgcgcga ggacatcgag catgaccgcg ctcgtccgcg
    11461 tgccggaggc aagggtgccc ccggtttcgg ggggaaaaac ggccggctgg atctcgatcg
    11521 ggtcgatgat ggcgaaggcg gcggtgcggt cgccggcgag ggcgagcgcg ccggccaggt
    11581 ggaacttcgc ctgcagcgac agcggcgcga tcggaatctg gcgcagttcg ttgagaatct
    11641 tgcggtcgag ctggtcggtg gaggcgagcg cgtaggccgc gtagacgcgg atctcggcat
    11701 cggcgtcggt caggtcgcgg ccgcgcgccc acgccaggag gcgcttgcgc accgcctcgt
    11761 agagatcgtc ctcgaccacg aaccccgcct gtcgggcctc cagcaggaag tgtccggcgt
    11821 acacgctcgt ccagggcgaa ggcgggccgc cgccgggcca gaagctgaac gtgccgtccg
    11881 cccgcatcat cgcccccagc cgccgaatcc cctcctgcac gaagtagtcg ggggcgttcc
    11941 cgccgagcag ttcgggactg acgaaacgcg ccagagactc gaaatacagg agcgggaaca
    12001 cccgcgaggt agtctgttca acgcacccat acgggtagga gaggaggtac tcgatctggc
    12061 ggcggaactg aagggcgttc agcgacgacg tctggagggt gaaccgggcg gtgcgcggga
    12121 tcacatcggc cggcggtacg atgcgggcgg cggcggtgtc ggtgacgacg cccgcgccat
    12181 agcgggtgga gagcgggcgg gcgggacggt tgggcaactg gaaggctgcc tccgtctggt
    12241 cggcgccggc ggttgcgcgg atgcggacag cgttgggacc aggcacgacg ccggccttga
    12301 acgtgaaggc caccgaaccc tcggcgccgt cggccagcgc gatcgtccgg gctttgtcgc
    12361 cgaccgcttc ggccggaccg tccagttcaa gctccacggt cacctcgccc gagtcgccgg
    12421 tgtgattgaa cacggtgacg agaccggtga ccacgtcgtt cgggctgacg aagcgcggga
    12481 agctctcctg cacgatgagg ttttcgcgga cagtgacttc gcgcacggcg gagccgaaac
    12541 ggttgccctg tgcggccacg gccatgagca cgagccggcc gttgaaggcg gggaggcgga
    12601 aatcgatcga ggcgcggccg gaagcgtcgg tcgtcaccag ccccgaccag agtgcgaccg
    12661 gttggacgcg gccggcggcg aacgggttga ggtggcggcg gcgggcggcc tcgaagaaac
    12721 ggtcgccccc gggcgccagg tggctctccg cggcctgtat gttggggtag aggaaggagt
    12781 agatgtcgta aggccgcagc gacggggcgc gctggccgta gaagaattcc agcgggtcgg
    12841 gggcggcgaa accggtcaac tggagaatgc cggcatcgac gaccgcgagc gtgagctgag
    12901 tcgggccggg gacgccgaca cggacgccga cggtcatggt gcggtggggc tgcatgacct
    12961 cgggggcctc gagagcgatg ttaagatcgc gctcggattt ctgcaggaag agcggggcga
    13021 tgccgaaagc gcgggccggt gccgagcggt caacctcggc ggccttcttc aacaccgtgg
    13081 ccgtgacgta ggcgttcgga aaatacccgt ccttgacccg gaggcggacc tcggccgtgt
    13141 tgtccggcat ggccatgaca aacgactcgt gcaccgtctc cttctccacc gtgaccagga
    13201 gcgtgccgcc gaagggggcg cggatctgta cgaccgcgtc ctcgccgggc cggtagaccg
    13261 tcttgtccag accgatctcg atgcgctccg gttcctccat cgaccacggg gcgtaccccc
    13321 agcctgacgc gtagagggag acgtcagccg aatggccgcc cgacgggtcg gcgatcacga
    13381 ggcggaattc gccgtgggtg tcgggggtga aggagacgac agccccggaa tccgccaggg
    13441 cgaccgtctg cgtctgaagg agcgtctcat cgcgctcaga cttgtagcgg tagcgtccct
    13501 gcgaatcgcg ggtgagcacg gtgttccagg tgacgcggta caggcgggcc tcggcggccg
    13561 cgagcggcac gggccggccc tcggggttga gggcgatgac gcgcgcggcg aacggctcgc
    13621 ccggcgttgc gtacccttca agatccagcc gcacccccac gtagcgcggg tacgggttga
    13681 tgatcacctc gcggtaccca ctgacggcgc ggccgccctg ctcggagacg aaggcgccga
    13741 tgagcgcccg gagagcggac gggggcgtga ggtcgcgcgg cacggtgtag ctgtacgtgt
    13801 gccggccgtc gccgtccagc gccgcgtcgg gaagattgtc cgtgcgcggc atgaatttcc
    13861 gctgcgagtc ggcgaaactg tacttgctgt aacccgaggg gacgaaagtg tgcggctcga
    13921 tctgcacgcg gccggccacg gcgtggttgg cggcggggga gccgaagagg aactcggcgg
    13981 cgacgttgat cgggatgttc tcgccgaccg tgtaggccgc ccgggccgtt tcgaccgaca
    14041 ccttgatccg gtcgggcacg aattcctcga cctggaaatc ggtgcggccg atctcgaggt
    14101 cgtcgccgat gcgtgcgatg gcggtgtact tgccggtgcg ggcgtattcg gggagagcca
    14161 gatcggtggc ggcgaacgcg ctgcggccgg tggtgaggcg gacagtctga aaatcgcggc
    14221 cggtcgggtc tttgatgttc aggaaatagg gaaagtcggc ggggaccgcg cccccggggg
    14281 cgcgcacgag gaccgccacg tgcgttgtct cccccggacg gtaaacgccc cggtcggtgt
    14341 agacgaacgc ctcgtaaccc cgggtgagca ggggccggcc cttgacctcg aagtcggtca
    14401 ggtcaaggcc ggtttcggcg aagcgaagat aggagaggtc accctcgtat tcgacggtga
    14461 tgacgtacgg ttcaaacccg gccgtctgct cgacgatctt ctcgaaaacc gccgcgcccc
    14521 gggaatccgt ccggccctgg agcaggagct ggttgttccg gctgtacagg cggacttcgg
    14581 ccccccgaac cggctcgacc gtcgagaggc ggttgaccca gaccacgagg taatcctccg
    14641 agaggcgggc gatgaggccg aggtcggtga gcagggcgag gcgggagtcg cggacccacc
    14701 gcgaggtcgg gctgtacgcg ctgatcttgt agatgccgcg cgcggtgtcg ccgacgatgc
    14761 cggcgacgtc gacagtcgtg gggaccttct cgttcttccg ctccggcacg gcgtgccgag
    14821 cggtgaagaa ggtgcggccg acagcgcgga tgtcggccgc gccccgctca aacggatcgg
    14881 cctgaccccc ggccagggca tagacgagat tgttcgcaaa gatgtgctcg atctcgaccg
    14941 cgagggaatc aacgttgatc gtctcgattt ccagaagtcc ggaattctct ctcggaaggt
    15001 agatgccctc cgagcggaac agcacggtgg gggggatgtc ctggatggtg acgcggccgg
    15061 agaagtcgtc ggccagaacg gcgcccgcct gcgaccggag gccggaggcg acgctgacgg
    15121 tgtaggtttc gccgggcgtg aaagcgccgg tcaggaggat ctggttgaag cgggtctcgg
    15181 cgcggaagtc gaccgcgggc tcgaccgcga caaaatcctc gacccggtcg gtcgcaatca
    15241 tggttgagaa ggaaaggtag accagcggcc ggatcccttc cgtgtacgtc cccacctcgt
    15301 gcacgaccag gcgctcccgg ggcggcacga cgaggtcgcc cgtgtggtcg cgctccaggg
    15361 gacggccgca gttcgggcag tcgacgccgg ctctgatcgt gaccaggtag acctgctcgg
    15421 agtcggaggc gggcacggga tcggtggcga gcgagatgat gtcggcggtg gccggaccgc
    15481 cggattcgtc gcccgcaccg ctccgcccgg cggtctgggt attgatgaca tcaagcaggc
    15541 tgaacgagag ccggcccgcc tcggcgttct tcttcccttc gatcgtcagc cgttcggtga
    15601 gatcctcaat gaagattggg tagttgaaac gcaggtcgac gtgaacgcgg acatggccgg
    15661 gccgatcctc ggccggcatg gtgtaggagg agacccccat gatgctgagg gggtcggtgc
    15721 ggaacgagaa cgtgcgcttc tcggcgatcc ggcggccgtc gcgccaggcg aggtcggagg
    15781 cgacggtcgc ggtgtacgcg gtggccgggg ccaggggaga ctccggaaag aatcgcagca
    15841 ccgccggttc aatccaccgg gcggcgccgg ccaggggcgg ctcgaaactc accggcggcg
    15901 cggcgaccgg ccggttcagg ctgtccgggg aaacgaggtc ggcggaaaac gtgattgtga
    15961 cattcgtgcg ccgatcggtc gtctcctggg ggcggaaatc tttgacccga acgttccggt
    16021 catcggtctg caactgggcc cacagggtca gggtggtggc aagggcgatg acggcgacga
    16081 gcgacaacag caccagcggc acccggccgc tcagacggtt ccacagagcg cgcatacaat
    16141 cctcctcaat caactccgga ccggccggcg tcggcccggg tcgaattaat gtattgcgct
    16201 cctctgtcgg ggacaagtct cgttattcgt tatacacgcg ccggggcgcc gcgcgccggc
    16261 gccgccaccc tcactcggcc gtacccgcca ggtactcccg cagagcatcg agaaccagcc
    16321 cccaggcgcg gtcaaagtac gcatagagac tgtcccagtc ttccccctcc ccccaaccga
    16381 ggtgggtgag ggtgatgcgg gtttgcgccg cagccggcgc ggccggggcc acacccgagt
    16441 cggcggccgg ccggtcggga gccagcggtt caaattcgac gaccacccag gtgcgctcgc
    16501 cgtcgcggcg ggacgggaac gaggggggag cgttccactc gaacgagagc atccgtccgg
    16561 gaaggaagct caggatcgcg cagtcctcgc tcccgcgctg gccgaccggg gaggccatcg
    16621 agaaatacag ctcgaacgag ccgccgaggc gcaggtcgac gttggctttc tcgaccagga
    16681 aggcctccag gccggcattg gtcgtccaga gggtccaggc cgagtcggcg gagacgggga
    16741 cgacaatctc tttgcgcagc gagcgcgcgc tcggacggga gacatagcgc agcggcgagc
    16801 ggtggtcggc gaggagggag agcgtcatcg gctgcagttc gtaggtgtgc acgccggccg
    16861 cgaccgaagg ctcgctgtcc atgagggcgc gcgcctcggc ctcggacccg gtgcgcagga
    16921 tgatcaggcc gaaagtgggg ttgaagaccg gcccggccgc caccacgatt cctttccgcg
    16981 tcaggtcctt gaggtaggcg aaatggttgg ccatgacctg ctcctcgcgc ggggtcatgt
    17041 cgtcgggcca cccggcgcgg gtgcccaaca ggcggacgaa gtgatagacg ggtttcatat
    17101 cgggattgtc tccttgagcg gcaggggcgc cggcggacag aatgagaagc aggctgacga
    17161 cgccggccgc gacggcgcgg cgcctggggt gaggatcaca gcggcacagc atagactaaa
    17221 gaatcctcct gtcggttgtt gcggaccgca aacggcagcg acaacatagg cgttggcgac
    17281 ggtcggcgca agcgcgagag gggcgcgggc cacgcgggaa aagcacttga cagactacca
    17341 gatccacgct atgttgaatt agaaatcagc cgaatcacgg atcattttcc gccgtccgga
    17401 tcggcgaccg ccgcttctcc tgcccgcgat cgagggggcg cgcctcggca tatcgatcga
    17461 cggtttcccg gccgggcgtc caccgagaag gatcacaatg agaacattca ttagatcttg
    17521 ggagtccttg tcaccgctaa gcgtttgcgg gaggtatggt atgcggacac gatttttcgg
    17581 atgcgtgctt ctgggatgtc tgctcctggg cggggccgcc ctggcccagg aggatccggg
    17641 tattccggat acgatgaagc tggtggcgac ggtgtgcccg gaagcggcga ccgggcagct
    17701 gaaagtggtg gtcgaactgt gggggtggac ggatcatctg gtgatgggca acggggcggg
    17761 gttcacctgg gatcatccca gcctcgtgat ggacagcgcg aagccgtcgg ccgctttgga
    17821 cgatgcctac gacgtcgggc cgttcttcta cgaaaacgac aatctcgcgc tgaccaacgc
    17881 caatcgccgc ttcctgacca gcggggccgt gatgttctcg tccggcctgc cgggcagcac
    17941 ccagtggcgg cactgggcga cctactactt caccctgacc gggtggtcgc cgagcgacgt
    18001 gatcacgctc gacacgctgt cgttcagccc cgggtcggcg tgggacatga cggcggtcaa
    18061 cgacgtcggc caggatgtct actttgagcc gcgctggaaa ggcgcgctcc acatcggcga
    18121 caactgcgag tactgcgtcg acgtggacaa cgacggattc ggcgatccga ccgtacccgg
    18181 ccaaacctgc ctgccggaca actgccccac cgtcagtaat ccgacgcagg cggatgcgga
    18241 cggcgacggt ctcggcgatg cctgcgacaa ctgcccgacg atggccaacc agaaccaggc
    18301 ggacggggac ggcgacggca aaggggacgt gtgcgataac tgcccggcga tcgccaatcc
    18361 ggaccagctt gacagcgacg gcgacgggaa agggaacgtc tgcgacaact gcccgacggt
    18421 cgccaatccc acgcaggcgg acggggacgg ggacggtttc ggcgacgcct gcgacaactg
    18481 cccgtcgctg gccaatccgg accaggcgga tgccgacggg gacggcgacg gcgatgtgtg
    18541 tgacaactgc ctgttggtgg ccaacccgac gcaggcggat gcggacggcg acggtctcgg
    18601 cgatgcctgc gacaactgcc cggccgtcaa caaccccgga caggaggacg gtgacgggga
    18661 cggggacggc gatgcgtgcg acaactgtct ggaaatcgcc aacccggacc aggcggacag
    18721 cgacctggac ggccggggga acgcctgtga caactgcccg acggtggcca acgccgacca
    18781 ggcggacggt gacggcgacg gacggggcga cgtctgcgat aactgcccta cgatcgccaa
    18841 cgccgaccag atggacagcg acggcgacgg tctcggcgat gcctgcgacg tgtgtcccaa
    18901 cgatcccgac aacgacgttg acggggacaa cgtctgcggc gatgtcgaca actgcccggc
    18961 ggtggccaac gccgaccagg cggacggcga cggagactcg atcggcgacg tctgcgacaa
    19021 ctgcccgacc gacagcaata ccgaccaggc cgacggcgat gccgacgcga tcggcgacgt
    19081 ctgcgacaac tgcccggctg aggcgaacgt cgaccaggcg gacggcgacg aggatacggt
    19141 cggcgacgcc tgcgacaact gcccggcggt ggtcaatggc gaccaggccg acggcgatgc
    19201 cgacgcgatc ggcgacgtgt gcgacaactg cccggcggaa gccaacaccg accaggcgga
    19261 tctcgacgac gacgggctcg gcgatgcgtg cgacaactgc ccggcgatcg cgaacgccga
    19321 ccaggcggat ggtgacggcg acaatgtcgg cgacgtgtgc gacaactgcc cggcggaagc
    19381 caacaccaac caggcggatc tcgacgacga cgggctcggc gatgcctgcg acaactgccc
    19441 ggcggtcgcg aacgccgacc aggcggacgg cgactcggac ggcgtcgggg acgcctgcga
    19501 caactgcccc gcaatcgcca acgccgacca gcttgacagc gacggcgacg ggatcggcga
    19561 cgcctgcgac gagtgcccgt tcgacgccga gaacgacatc gacggcgaca atgtctgcgg
    19621 cgatgtggac aactgcccgg cggtcgcgaa cgctgaccag gcggatgcgg acggcgacaa
    19681 tgtcggcgac gtgtgcgata actgcccggc cgcggccaac ccggaccagg acaacgcgga
    19741 cggcgatctc tacggcgacg cctgcgacaa ctgcgcggcg gagccgaacg acgatcaggc
    19801 ggatgcggac ggcgacaatg tcggcgacgt gtgcgacaac tgcccggcga cggccaatgt
    19861 cgaccagacg gacggcgacg gcgacggggt cggcgacgcg tgcgacaact gccccgagaa
    19921 atacaacccc gaccaggccg acaccgacca ggacgggatc ggggatgtct gcgacggctg
    19981 ctgcgtcggg atgcggggag atgtgaacgg cgacggcgaa ccggtggcca atatctccga
    20041 cgttacctac ctggtcaact acctgttccg tcacggtccg gagccgccgt gtctcgcgga
    20101 ggccaacatc aacggcgacg gacttgagaa ggtggatgtg gccgatctga cccatctgat
    20161 cgcgtacctc taccggatgg gtccggcgcc ggccaactgc ccgtggtgag acggccggct
    20221 gttgtggaat tcgggaagca cccccgcccg cgcgggggtg tttctttgtg gaacggccgg
    20281 gcctccggaa atcggaggtt gccaggggcc gcgatattgc ctaccatgtg ccgcaaaaat
    20341 cgagaaattg accgagaccg tccccaattg acgggacggg cgaggaaagg accaagcatg
    20401 ggtgtacgag cgggagtcgc cgcggcgctg gccgcgctgg tgatcgggtg ttcatcggga
    20461 gacaagcagg ccgggaccgc gggcgggcca agcgggatgg cggaccggat cgcccgggtg
    20521 gatccggcgc agttcgacct gaccggccgg ccggtcgcgc tcgcgggaat gacgtttcag
    20581 ccgcccgccg cctgggtcga cctgggaccg tcgggcatgc gccaggcaag ctactacctc
    20641 cgcccggtca gcggagatgc cgattcggcg acggtcaccg tgttctactt cgggccgggc
    20701 ggagggggat cggtggcggc caatatcgac cggtggatcg gccagatggc gaccggggag
    20761 aaggacaatc cctcggaacg cgccgagcgc tcatcgttgc aggcggacag cctcccggtc
    20821 cacgtgatct cggtcggagg gacctacagc ggggccatgg gcgggccgat gggcggcggc
    20881 ggcgaagccc acgccgacta ccggatggtg gccgccgtga tcgaggcccc cgaaggaaat
    20941 gtcttcttca aactgaccgg accggaaaag acggcggtgg aaatgcagag tggatttttc
    21001 gccatgctca aatcggtgaa aagggccggc tgagacgatc ggtcgggcgg gaacccgggt
    21061 gaaaacatgc ggccgccccg agcggccggc gatcgtgttc ccggagcggg cggacgtgag
    21121 gtaagtggaa ccggcccagg cggcgatgcg gacaacgccc gccggcagag cgccgattca
    21181 cgaggggcgc cgccaaagat ggatctccat ctcggcccac ctgacagggc cccccgagca
    21241 cggtgagcgc ccgagaagtc gcgactcgaa aaggagttat catccaggtt gcatcggcgg
    21301 ccggtccgac cggcggtgcg ccggtcccct acttttccct tgtaagtcgt tgatcggttg
    21361 tatattcttg accgtcgagg cgaaacagcg cttcacctga atcgtacagc agaaagagat
    21421 atcggtgcag gacaacgacg cccaggttat cgagcaggcc ctcgcaggca accagcaggc
    21481 gtaccgtatc ctcaccgagc ggcaccggcc ctcggtcttt catatcgtct acaagattgt
    21541 ccgggaccgg gaaaccgcca acgacctggt tcaggaaacg tttatgaagg ctttttcctc
    21601 gctggcctcg taccggtcgg agtaccggtt ctcgacctgg ctgtacaaaa ttgcggccaa
    21661 ctgcgcgatc gaccacctgc gcaaaaggcg cattcaggtc ctgtcgctgg aggggcagat
    21721 ggaccgcgat tccagcgggc ggacgattga agtccccgac tactcctacc acccggaaca
    21781 cgacctcgtg cgcaaagagc agcgcttcaa tatcgaagaa gcgatcgact cgctgccgaa
    21841 gaagtaccgg gaggtgatcg tctaccggca caaggaagat aaatcctatg aggaaatagc
    21901 cgatctacta ggcataccga tcggaaccgt caaggcccgg attttccggg cccgggagct
    21961 tctgaaaaag aagctcaagt cgatctgaac ccggaaaggt aaagggtgcg caagatgcct
    22021 cggctttccc gcacagttgc ccgccttctg attctcctgg cggccaccgc gccgccggca
    22081 gcgggggaag aattcacgtt cgattaccag aaaatcgtcg aggcccccga gccggtccgt
    22141 ctcgatctcc ggctcgtgcg gggatcggtc gagatcacgg gcgctgagga caaccgggtc
    22201 gtcatcgagg cggtcaagcg ggttcgggcg ggcagccgcg acgaggccga ggaactggcc
    22261 gaccacatcg aaatcaagac ccgccttaat gggacggcgg tggaagtaca gacgaattac
    22321 ctgggcatca cgagccgcgg gcgttcgttc tggcagaaag tgttcgggaa gggagaggat
    22381 gcgcccggag aggtggccta caaagtctcg gtgccggtcc gcagcgacgt gaccgtccag
    22441 ggactcgcgg ccacgatcac cctcgcctcg atcgagggcg cagtcagggt ggaaaacgag
    22501 tccggcgcga cgcgggccga atatatcttc ggcccggtga ccgtcagcca gccccaggga
    22561 gatatcgacc tcctgtggat cgagggggac attcgcgtga aggcgacgac cgggacggtg
    22621 acggtaaacc aggtgcgggg ggcgctcgat atcgccaccc acacgggaga ggtgcatgtc
    22681 cggacggagc tggacagccc gagggacttc tttgtcgcga ccaccagcgg caagattacg
    22741 tttgcgattc cgccggcggc ctccggccgc ttcctcgtag agacggcgag cggaggaata
    22801 cagagccaag tgccgctcgt gatcgagtca gtgacgcggc agcagttgat cgggagtttc
    22861 ggccgcggcg ggccgaggat caacctgacg acgtcatcgg gcgacgtcac ggtggcccag
    22921 tactgatcgt tcgcggcgtt ggaacatagg acgtggccat gcgcaccgca gtgcgaaagt
    22981 tcggtttgtc ccttcttctc gcgggcctcc tcgcgggccc ccgccagttc ccggccgtcc
    23041 tggcccagga ggcgccggat gagcgtgcgg cggaatcgtc gctcgccgcc gatttgcctg
    23101 ccgacgtggc cggtccggcc accgcccccc ccggcatcgt ttttttcgaa atccgtctct
    23161 ccgaagatcg cgccgtggcc accgacacct cgggtcgcga atgggtgtac gacttcaacg
    23221 ccgatgtctg ggtggaaggg acgccggggc aggagggggg aaggctcacc gaccggctgc
    23281 agccgcgcac caccctgccg atcgatctcc gcgccatccg cctggtggcg gtgaagccgt
    23341 tcgagcgctc ggtgacgatc gggtatgatg agtatgtcga cggcgacatc atcgccaccg
    23401 gccgggttac ggtcaagggg tgggtgaagg gggatgtccg gtcgattgac aaacgggtac
    23461 tggtgaccga gaccggacag gtggacggca gcgtcgaggc gcccagcgtg atcattcggc
    23521 cgggcggagt ggtgagcggg acggtgcggg agacgggcgg cccgctcgac gtggagagcc
    23581 tgacccgggc cgtctcggtc gatgccctcg ttttggcgac cgccctggcc tgcgggctgc
    23641 tctttttcgg ctttctcgtt ctggcggtgg ctccgcggca ggcgggacgg atcgacgcct
    23701 gcatcaccgg ccaccgcgtc cgcacctgcg ccctggggtt cctgttttcc ctcctgctcc
    23761 ccgtcatcat cgcgctcttc atcatcaccc tcgtgggtat cctcgtcgtg ccgttcgtgc
    23821 cgctggtgta tgcgggcgcg atcggaatgg ggtttgtgtc gttcggcggg actatcggcg
    23881 ggtgggtgct gaggcggctc accggggccc gccaggggac gcagttgcgc accgcgctgg
    23941 gaacgcttct gctggcgctg ctctggctcc tcgtcattgt cctgctcggc gggggtaacg
    24001 ccttcctgct cgggctcggc tacgccctcc tggcggccgc cattccggtc acaagcttcc
    24061 cggtgttcgc cgggattggg ggggcgatcc tgactcggtt cggtttccgc gactatgtga
    24121 gctggaagga gcgcaagccg gccgacaccg ggccgccggc gccggcgccg ccgccgctgc
    24181 acgaaccgcc gccgatcatc accccttggc cgccggcgcc cgagcccccc gaacgccgcg
    24241 aacctccgca ggagtgagcg gggcaacatt cgctgccccc gtccgtacca tccctcagca
    24301 ggtgtcgtga ttgatcggtc ttggaccgga agggagatta tgcgcggcgt tttcagattc
    24361 tggctcctag cggccctcgc ggcgttgccg gcggcggccc tccaggcgcg ggagcggaaa
    24421 aataccccgg tggcggcgga aggggcggaa cggatcaacg tgcaaattga cttcgcggcc
    24481 ggcaagctcc ggatcgatcc gggggagatc gccgaggcgg ccgtgttcga cttcgcctac
    24541 gacgaggaca aggtcgactg cgccgtcgac tatgaagtgc gcggcggcgt cggaatgctt
    24601 caggccgaga gcgagcggct ctcggggagc gggggcgaca cgggcgacaa tgaatgggag
    24661 gtcgtgttct cgcgcgccta tccgatgaac ctgagcctcg acatcggggc ctgcgatgcc
    24721 gagatcgagc tgggcgggct gcggctggag gaagtcgctg tcgaattcgg cgccgcctcg
    24781 gccgaaatcg ggttccgaga acccaacccg gcccgcatga gagaactgcg gttcgaggcc
    24841 ggcgcgagct cggtgacggc gaccgacctg ggcaacgcca atttcgaatc ctgtgagttc
    24901 gagggcggtg tcggctcctt cgatctcgac ttccgcggag agtaccacgg ggagtcggag
    24961 atccgggtga aggtggggct gggctcggcc gagatcactc tgcccgtgga catcccctgc
    25021 cgcatcgaag gaccgggggg cggcctgctg tcgtcggtcg agattcacgg cggcggcctg
    25081 cgggagattg ccgacgacgt ctgggagtcg gatgactacg ccggagcttc gacccgtctc
    25141 cgactgattc tcgagctcgg gctcggcgcg gtggatgtgc gcttccgctg acgcgggcga
    25201 acggaggacg gcatgcgcgg ccgggcggtt tgagccgccc ggccgtttct tgttgcgccg
    25261 ggggcgttaa aacttgccgg gggcgggcgc gggttctata ctctccgcgg accggagcga
    25321 ccggccgagc ggagccgccg atgccgatga gcaggcggcg gccgcgcggc cggggaccct
    25381 tggggctcac cgaacattcc gcgcacaggc gtgttataag ggcacgagaa aaaacgatgc
    25441 aaggagccag gatgcacctc aggcaagtcg gggttgtgct gctgttctta ttcatgtgtg
    25501 cggccgtgac acgggcggcg gcgccaaccg atcttcagga acgcatctac tatgcccgcg
    25561 accgggtgct cccggcgctc gtgcatatcc agccggtcat caggaactac gacaccggag
    25621 aactcgagaa gcaggccgtc atcggctccg gggtgatttt tcacaaagac ggctacgtcg
    25681 tgaccaacta ccacgtggcc gggaaagccg tgcggattat ctgcaccctg tacgacaagg
    25741 agcaggtacc ggcggagttt gtcggggggg atccaatgac cgatctgtcg gtgatcaagc
    25801 tggacctctc aaagtatgac aagccgctgc agccggcgga gttcggcgac tccgattcga
    25861 tccatgtcgg ccagtacgtg ctcgccatgg gctcgcccct gtccctctcc cgctcggtca
    25921 gcgccggcgt catctccacc cgcgaccggt atttcgacga tgacgtgcgg ctgcctaccg
    25981 gcgagcagac cggaaagtac aatctctgga ttcagacgga tgcggcgatc aacccgggga
    26041 attccggcgg cccgctggtc gatctggacg gtcgggtggt cggaatcaac tcgcgcgcca
    26101 ccctgttcgc caacaacatc gggttcgcca tcccggtcaa cgtagtcaag gaagtgacgg
    26161 cggcgattct cgatcacgga gcggtcgaac gaagctggat cggggtccac gcccaggcgc
    26221 tccaggagct ggccgatttc ttcggcgccg acagcggcga aggcgtgctc atatcctcga
    26281 tcgaccccgg gtcgccggcg gaggcacgct tcctgaaagc cggcgatatc atcacggcgg
    26341 tcgacgggcg cccggtgtcg gcccggttcg tcgaggaact gcccgcattc tacaaccgga
    26401 tctcccgcat ccccccgggg agtgaggtga cgctctcggt gaaacgggac ggcgcgacca
    26461 tgccggtgcg cctgcggacc aaacgcctcg gcgacctgca gggggaagat ttcgaggcca
    26521 aaggatgggg gtttacggtg aaagcgatca cccggcagat gcagattgac gcgacccttc
    26581 ccgacaccct cggggtcatc gtggtcgggg tgaagcgcac cggcccggcg ggggaagggg
    26641 gcctgcgggc gggcgatgtg attcagaaag tcaacgacgc tgcggtgggc tccctcgagg
    26701 agttcgccgc gcgctatcgg gaactgtcgg cgctggacac ggagaggatc ctgctgacgg
    26761 tgagccggac gggatcgacg cggtttgtgc tcttgaaggc ggacaagaac caggaggtta
    26821 ccccccatga gtaagcgcgg ccacgggcgg atggtaccga ggcttgctcc ggcggcggtg
    26881 tgggcggccc tgtggctgct ggcgccggcc gctgcattcg ctcagcattt cgatttccgc
    26941 accctcgaag agaaggtgcg cgcgttcacg gtcgtcatcg acatggatgt cgagatctca
    27001 ttcggggtcc actccaccga gcagaccgac cggtatctcg gcacgattgt caacgacagc
    27061 gggctggtta ttttcgacgg ttcgggggtg gggaccgaaa ccgctatccc ctcattctcc
    27121 ggattcgccg tcaaaacccg gccggtccgc attacggtca ccagcatgga cggaaagacc
    27181 tacgcgggcg aatacctcgg ctatgaccgc tacaccaact tcgctttcct ccagatcgac
    27241 ggcaaagggg acacctttac gcccgtggcg ttccggggcg gccaggagtt ccaggtcggg
    27301 cagtgggtcg gcctgttctg gctgctcccg gagttcgtga gtcccccgct ggcggcggat
    27361 gtgggcatgg ttgcggcgcg agtgacttcg ccggaggaga tggcgctgac cgtgggattc
    27421 agttcgctcc aggcggccag tgtcctcttc agcgagcagt tggagccggt cgggatgctg
    27481 ggagcgctgg cggatccgac caccgggggg gatgacggct ccgggatgct cggcactttc
    27541 ggggatgccg ggataccgct cctgggcgtg gtcacggcgg agcgcctaac gcgcctgatc
    27601 gcggaaccgc cgcgccaggg ggagggcggc cgcggctggc tgggcatcac cctccaggcg
    27661 ctgacggccg atatcgccgc gttctggaat ctggaaacgc tcggagggat catcgtcaac
    27721 gaggtcgtca agggctcccc ggcggaggaa gccggtctgg aagtggggga cgtcattttc
    27781 gaaatcaacg gccaggcgat cgatgtggac aaggaagaga agctgccgct cttccagcgg
    27841 accatctctg acctcggccc gggagcggcc gtcgagctcg gcgtgctccg actcgggccc
    27901 agcacgatcg acacccttca cgtgctggtg acgctggcgg acgcgcccat cgccgcggcc
    27961 gacgcgaagg aactgaaggt cgatgagttc gagttcacgg tccgcgacct cgtcttctcc
    28021 gattacatga tcgccaatca ggatcccgag aagttcgaag gggtcgtggt ctcgaagatc
    28081 gaggcgggtg ggctggccga actcagcggc ctgcagatcg gcgacgtgat tcagcggctc
    28141 ggctccacac ccgtgcggtc ggtggccgac gtcgagagtg tcatgaccgg aatgaaggtt
    28201 gaaaagcccc gggaggtcat tctcttcgtg tggcggggcg gcaagacaat gttcgtgaat
    28261 ttgaagaccg actggccgta aggcaaacgc ctgatccggc agcaagaacg gctaagccga
    28321 cccggcgacg ggtcggcttt tcttttgcgt agctggatcg ccccaaccgc aagcgaatgc
    28381 acaacgcaca ggccccgcca cccggcataa atctccggag ccgatagccc ctctctcgtc
    28441 gacaatcgta agttgttgac ttcgcgttaa ttgcgggagc ccaccggcgg cgggccgcgt
    28501 cgctcacacc gccggcgttt ttcggggacc cggcatgaag gttgcccaaa ggggggccgg
    28561 acctgccggt ctcctccaac tcgtgcgcgg acggatcggc agggtcagaa gacgccaaag
    28621 cacagaagga tgataaggaa agggattgca gtatgagaaa gctgttgtta acggtcctgg
    28681 cggtgctcct ctgtgcggcc ggcagcgagg cggttccgcc ggtcgcccca tgggcggcgc
    28741 aggtcacact ggatacggtc gcggcccggc ccgggcagca cgtggccgta ccgattcgac
    28801 tgagcaacaa cgaccaagtg agcaccggca tctatatccc gctccgccta gcgagttcct
    28861 ccctcacggt ggattcggtc agcttcgtcg gctccatcat gacggccgat ttcaccggca
    28921 tggcccacgt cagtgcgtcg ggtctcacaa cggtggagat tgcgctggcg ccgaatctgg
    28981 tagcgcccat tccaacgatt ccggccgtca acgggatcat tgcgcgcctc tgggtgacgg
    29041 tgtcgccctc ggcgccgctc gggctggccc ggatcgattc cctggcgatc gactcggcgc
    29101 tttcggatgg cggaccggcc gcccgcttgt ggaagtacgt gcagggagcg acggcggagg
    29161 gggtgacgat gatgccgggg ttccgggccg gtggagtgct ggtggagtcg ccgaccggga
    29221 ttgaggacga ggaaaacctg atgccggggc ggtttgagct ggcgcagaat tatccgaatc
    29281 cgttcaaccc gagcacgacg atcggcttca ctttgccgac cgcgcaggac gtgcagttgg
    29341 ttgtctacaa tgtacttggg caggagatcg ccacgctggt gaacggtcgg atggcggccg
    29401 ggagccacca ggtgacattt gaggcctcgg ggcggccgag cggcgtctat ttctaccggc
    29461 tgacctacga ggatggggtc gagacccgaa aaatgacgct cgtcaaataa gaaatcaggc
    29521 cttcgtgccg atgattacag ggatagcggg attctccgct atccctgttt ttctttgccg
    29581 atgggcacga tatgacgcaa gctgtcgaca acccggcgcc ccccaaggcg gtccggatcg
    29641 ggccgtacct gctgacggcg aaaatcggcc agggagggat cgccgtcatc tacaagggcc
    29701 ggcaggaatc gctcgaccgg gacgtcgcga tcaagattct ctcgccgaat ctttccaccg
    29761 acccggacat tgtccgccgc ttcgagcgcg agtcgatggt catcgcccgg ctcaaccacc
    29821 ccaacatcgt ccacatcatc gacaaggggg aggcgggcgg ccgctactac ttcgtcatgg
    29881 actacatcga cgggacctcg ctgcgggagg tgatcgactc ggccaagatc ccgctgcgga
    29941 cgaaactcga gatgatcgtg caggtgtgca aggcgctcga ctacgcccac aagaacggcg
    30001 tgatccaccg cgacatcaag ccggccaaca tcctcatcga ccgccagggc aatgccctgg
    30061 tggcggattt cggaatcgcc cagattgtcg ggacgccgga ggccgagatg acggcttctg
    30121 acgttgtgat ggggacgctg tcgtacatgt cgcccgaaca aaaagtcagc tcagccgggg
    30181 tggaccacac caccgacatc tacgccgtgg gcgtgatcct gtacgagatc ctgtgcggga
    30241 aaaagccgct cgggaagttc aaactccctt cggagatcac ggccgacctg ggagaggcct
    30301 acgacgggat catttcgaag tgcctcgccc aggaccgggg cgagcggtac caagcggcgg
    30361 tcgagctcaa ggacgcgctg cttgaggcaa tggggggcgg gagcctgcgc ggcgacacct
    30421 ccgacctgac cgtctccggg accgaatcgt ttctggggaa atgccggtac ctcgacacca
    30481 tccgcgagac ccagttcggc tcgaccatcc tggtggaaaa ccgggtcaac aagcggctct
    30541 acgtgatcaa gaaacatcac aagggagagg cggggcgcaa ggaggccaag ctcctggcga
    30601 cgctcaagca cgagcacatt gtcgatatct acggagcggg cggggaccgg aagtcgacgg
    30661 tgattatcgc ggagtacatg cagggcggct cgctggccga ccggatggtg cggaagtacg
    30721 actgggagaa agcgctcgcg atcatccgtc aggtcgccgc cgccgtcgac ttcgcccaca
    30781 agaacaacat cctccacggc aatctccgcc cctccaacat cctgtttgac aaagacgacg
    30841 tcgtgaagct ctccgatttc gccctgccga ttcactacga cggtcccaag cagaagaact
    30901 ggtacagccc gccggagcgg aagagctcgc gccaggggga tgtgtacggg atcggcgtga
    30961 tcctgcacca gatgctcacc gggcgcaacc cgcggtacga cgagaacgac aacctggatc
    31021 tcgacgacat ccgcatgttc ctgcccgaag agatcgcggc catgcttgac cggctgctgg
    31081 cgatccggat cgcgcggcgg tacaggtcgt gcgcagagtt cctcctggac tgggaggatt
    31141 tcgaccgccg gcggcaggag gagcgccggc agcaggagag ccgcgcggcg gcggcgccgg
    31201 ccgcggtccc cggaaaaccg gtctggctct acctggcgat cggagccggg attgccttcg
    31261 ccctgttcac cgtgctctac ctcgccggcg tcttcagcct ctgactaccc cactcaccgg
    31321 ccggtcctca cccgaggccg gcccattctc cgccgaccag ggccgccggg aaagcttgtc
    31381 ttgcacccgc cgccggtgta tactggcgta agtcttcctg cggagggaac acggcgccat
    31441 ggcggcaaca atgcgaagag agttcatcgg ggtcggcatc atctacgcgc tcgctaccgg
    31501 gctggtgctc ctcgcttcga ccgttcggcc gagggggctc tgggctttct cggccgcgtc
    31561 gtacttcggg gatccgtggg cgtgggcgcc ggcggcggcg gccgtgctgt gggccgccgc
    31621 ggtccttctc tatgggcggc gggccggcga cgggggggac gcgtccgacg gcggtcgccg
    31681 tttcgcctgg tgggcggtca gccacacggc gcttatggcg gcgctgttta tcggcctgcg
    31741 ggccagaacc cattttctcg gcgacggcta cacgctgctg gggacgtacg ccacggagaa
    31801 tccgctcatc aagtcgcggg cgctcgggga agcgctcgtg cacctgtggg tgcgcgacct
    31861 ggtcggcggg ccgaccaagg cggcggcgct gctgagtttt caggtgctgg cggtgggggc
    31921 cgggctgatc tacttagggc tggtctggtg ggcggccgcg cggctgtttg aacaccggcg
    31981 cgagcgcctg ctctttgcgg tcgggatgac aaccccgggg tacatgctgc tgtattgcgg
    32041 ctacgttgaa ctgtacgccc tatttgtcac ggccgtggcg gctttcg
//
LOCUS       DAOPOE010000024        31979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1837_length_31979_cov_4.490853, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000024 DAOPOE010000000
VERSION     DAOPOE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31979
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_31979_cov_4.490853"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(891..2210)
                     /locus_tag="PKM94_05075"
     CDS             complement(891..2210)
                     /locus_tag="PKM94_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD66047.1"
                     /translation="MKHTGRPLRERLRTTGVAAAMLVAFAVSGAAHDYLPGPPQDRPI
                     LLRNGKLFTVANGVMDGADLLFESGRITAIGRNLEAPEGAQVIDVTGLHVYPGLIAPH
                     STLGLSEIGAVRATEDTQERGLAKGELKAYAAYNPDSEIIPTVRANGITTALIVPGGQ
                     LVMGRSSLVNLDGWTVEDALVRPVVGLHLSWPRASTTSEWWMEESVEEQKKARQEEQA
                     QLRGVFDQARAYYLAKTADYAIPVDSRWEGFLPLFDRTMPLFVHADDYRQIDQAVALC
                     REYGFRMILVGGREAWKMAPLLRDNGIPVIFGPTQRLPMREDDDYDLNYKIPALLHQA
                     GVEFCFSAGGGATGVRNLPFQAGQAVGFGLPWEAALRSLTLTSAEILGIEGELGSLEV
                     GKRATLVVSDGDLLDPLRARVRMEFIDGRAVDLSSKHTELYQKYRAR"
     gene            complement(2194..5277)
                     /locus_tag="PKM94_05080"
     CDS             complement(2194..5277)
                     /locus_tag="PKM94_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD66048.1"
                     /translation="MKSNHSVIILALCAGSVLAASGTTPELGIRDHTPDLTALVNARV
                     VISPDRTIDSAVIVFEHGVIREVSRSARVPEGALVVDLGGRVVYPGFIDPHSDYGLLA
                     EEEGHGQGGRIAWGGPKYEADRIGGSAWNEAIRAEIDWVDSFRPDSTAAQKYLGAGIT
                     VTQTTRQEGIFRGRPAVVLLRQGLPNNQVLRARSYPALSFYKGSSAQDYPSSQMGAIA
                     LIRQTLLDVDWYRAARAAWERNPGQEMPEFNAAIEALGRQADTMIFVAADDKEIIRAA
                     RLAREFGRTFICVGSGCEYERAEEIRGELVELIIPVNYPEAPEVGSLEDELEVTLEDL
                     RHWERAPGNAARLAQEKIPFAFTAAGLDDPSEYLSRVRTAVRYGLPPETALRALTTVP
                     ARLCGVDRYVGTLEPGKLANLVVCSGDLFADTTRILAVWAAGQKYEFEPLHPGEYRGQ
                     WQLRTAADSATLALEGAPSKLAGRLVAGSDSVELGEIEAARDRITFRADSLWGKPGVC
                     RFSGRLFGDSLRGFVAFPDGRRSEWQAVRTAPLAAEKDTAGAPSPAPEPEPFVSRLTY
                     PNRAYGLESVPVPQDVLVRNATIWTSDEAGVLEGADMLVRGGKIAALGRNLESPSGAA
                     VIDAAGKHVSPGIIDEHSHIAIDGDVNEGTHAVTSEVRIADILDPDDLNIYRELAGGV
                     TASRLLHGSANPIGGQAATIKLRWGLPADSLVFAAAPKAIKFALGENVKQSGWGERFT
                     IRYPQTRLGVDAIIRDAFLAAQAYEQRWDRFRRLGKSEQERTIPPRRDLQLEPLADVL
                     HRRMAIHCHGYVAPEMLMMIRLAQEFDLELQSFAHGLEGYKIARELADAGVGVGSIPD
                     WWAYKFETYDGIPHSPALMSEKGVVVAINSDSPELARHLNQEAAKSVMYGGMKPEEAL
                     KMVTINPARLLGVDDLTGSLRVGKDADFVIWSDNPLSIYARAEQTWVDGRKFFDLATD
                     SLLREQLQTEKQRLIQKVLRASGGGSPKRPPRGRPGPPPDGGLSSTEGGAREAHR"
     gene            complement(5480..8458)
                     /locus_tag="PKM94_05085"
     CDS             complement(5480..8458)
                     /locus_tag="PKM94_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOD66049.1"
                     /translation="MVALAAAATGGLYASAPLPDRSADLPIGITLAAASLAFVPNVGQ
                     YAAPVTFRAENGDAIVWVAGNEVFYEFIEPAEGTPPPDDLSRYGYYPAGEAARFTYRL
                     VRVSFAGANPAPAVVGREPMPGLRHYLVGADPADWRTHVPQFGAVAFEDLYDGIDLVY
                     RAAGNALEYDFHIDPHADPSRIRIAVQGAERTVIAADGRLVIETDGGRIEEYAPVVYQ
                     LSGSSRLAVPAQFVILGDNTFGFRLPAGYNPDLPLVIDPVVEYSTMLGGASNDYCRSL
                     AIDADGNAYAAGYTSSVDFPVKAAYDGSYNGGGTFGYDMVVTKISPLGDSLIYSTFIG
                     GATGDDRAYGVAVNGAGEACAVGQTSSSDFPVVSASQGALAGGDDAALLRLSASGDAL
                     LMASYLGGTASDVANGVAVDNSGNLYVVGRTESSDFPLSGSPFDNVFGGTQEAFLGKF
                     SPTGPRVYSTFLGGSANDAALATAVNGTGEAFVTGYTLSTDFPVLSAYDATNAGGSSA
                     GDVFVTRFNAAGSGLVYSTFLGGAADDAGLAIALDSADNAFVTGYTLSTDFPVVAPFD
                     ATHNAYLDAFASKLGPSGNSLAYSTYLGGSGNDLGAGIDVDYLGFAYVTGNTFSPNFP
                     TRAAVDSTFAAGADAFATCFGQPGDTLVYSTFLGSGALDFGYAVAVDTGQNAYIGGYT
                     SSTTFPTVNAIQGTHGGGYDMFVLRLAIDEWICVDTDSDGFGDPGHPENECPTDNCPL
                     VANPDQADADGDGAGDLCDVCPGFDDSLDADADGVPDGCDVCPGFNDNVDTDGDGVAD
                     GCDLCPGFDDAQDGDSDGVPDSCDNCPARANAGQADDDSDGLGNACDNCPTVGNPDQA
                     DIDADGLGNACDPCTDTDGDGYGNPGYPANTCPTDNCPYTYNPSQADADSNGIGDACD
                     AGCCVAPRRGNVNGDAADNISIADVTFLISFLFQGGPTPGCLEEANINGDSSGTIGVS
                     DLTALTAYLFQGGPPPADCP"
     gene            complement(8764..13398)
                     /locus_tag="PKM94_05090"
     CDS             complement(8764..13398)
                     /locus_tag="PKM94_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66050.1"
                     /translation="MRAHTGRHFPLSATLILVILILASAARTAEAETREPVFVPGEMI
                     LQLSSAKAAGQLASDFAALDLRPRQLLSRRLNIWLYEYRADGHKDTAAHEEVLARVRR
                     HPAVELGQFNHEVALRSTFPDDSSFGQQWALHNTGQTGGVADADIDAPEAWDLTTSGT
                     TADGDQIVIAIVDRGYDLNHPDISFFKNTHETPANGIDDDGNGYIDDYDGWDAYSNDG
                     TLPASDHGTHVAGIAAAIGNNGAGVSGVNWQVKVMPIAGSSTSEATVVAAYAYALEMR
                     AQYNESEGALGAFVVATNSSFGVDYGDPVNFPVWCAMFDSLGAYGVLSAAATANIGMD
                     IDANGDVPTACPSDYLIAVTNTTSSDVRNSGAGWGAASIDLGAPGTSVYSTLLGGGYG
                     NKTGTSMASPHVAGAVGFMYSAASPVVIDLARRRPDSVALLIRQAILDGVDSIGSLTG
                     VTVTGGRLNLRTAAVLVQAIPTGVTIVHQPLADTRDTVNDYAVAAQITSDTTLAPDSL
                     RLHYEIASVWTSAILSPAGGVQYSGAIPAQPPGTTIRYYLTAADVEGKADTTDIFTFR
                     VIDYAVSVRPDWQTQTGLVSDTMWFDLWVVNTGLRADDYTLGIGQAKWVTQIFDSTMA
                     GPISATGAVGAEDSVKVKVRVLIPASTYLAADTAVMAVLSTANPAVTAAGAAVTVSDG
                     PALAVPFSEEFAAAAFDSSRWAGVVEAEISEAGLAEPSPPYSLNLDGNRYGADTVLSM
                     AIDLRGQTAAMVGYAYQRTGGGDSPEAGDDLVVEYFDSTGTWRLIRQHLGADPDMTTF
                     EEVEFSLPADAFHRRFRLRLRCTGTIGVFDDWFVDDVYVGPPPAYAVRVTPPQSRQYG
                     FAGDTVMYLVTVHNRGLNTDRYHLFAEDLGGWPVAAYDSLGQGIITVTDFVPSGDSSR
                     FAAKVPIPADAAANDASAARVRVVSLSNTSVADTAALETVSSGPPGGFPWYESFPEDT
                     LIAFRWPQNSGATVTASAVDPPTPPYALDLDGGIDTVVSQVLNLSAVGEANLSFYYQR
                     AASDPPEAGEDLWLEYKTSVGQWVQLDQLAGGGGAMNYFAQRVIPLVPAALHSGFQLR
                     LRSFGSCANCDHWYIDDIRIDYAPDIAVAPAAIEVTLEQGDSAAAHLIIENEGLGGLD
                     YSLSRVPATATLPFARLAAEDGLEPALRSYPDGFDDYAEPKGGEDPRIGAPVTRDAGG
                     PDAYGYFWVDSDEPAGPTFAWIEASTTGINIIGTLTDDSFSGPYAIGFDFPFYDSTYS
                     TLFVGSNGIIGFAPISLNSRFKRHLPDTTAPNALLAWLWDDLNPTDGANPGAAVYVDA
                     TPARCVIQFSAYPEYLAGAGDVVNAEVILYPDGSIVYQYLSIAPGFDVASCAVGIENP
                     AGADGLEVAYLTPYLKDSLAILFTNPSQWLALERTAGSVDPGGADTLALTITTANLGS
                     GSYGLILLVTSNDPDESPWSVPATVHVTLPPQYVCGDLTGNGGTPNVADLTYFVNFLF
                     RGGPPPADMAAADIDGSGGLPTVGDLTYFIGFLFRGGPPPICENGRHRAQ"
     gene            13744..14484
                     /locus_tag="PKM94_05095"
     CDS             13744..14484
                     /locus_tag="PKM94_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOD66051.1"
                     /translation="MRIGLILGPAAAPGLLAPADLQPGHPAVTAAAAVAVLMAVWWIT
                     EAIPIPAGALLPIALHPVLGIMKGDAVAAKYVSSVIFLFIGGFIVALAMERCGLHRRI
                     ALRIITLIGASPRCLLAGFIAATFVLSMWISNPAATMMMIPIAMAIVLKVEEGAPGAP
                     TARFAAVVWAVLTALYAPSAGELKEGKEIFAWLGSLRWLGRRRGEGQREIRVEKARDR
                     FGFCNLLYANTEGSCEEAAANAWNPVSP"
     gene            14554..16683
                     /locus_tag="PKM94_05100"
     CDS             14554..16683
                     /locus_tag="PKM94_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOD66052.1"
                     /translation="MKLSHSLALILIAVALVASAALAQETAVAPHAPVAAMIPRVDTV
                     NGNIVRIDDYYWLRNRGDSAVMAYLEAENAYTEACMAHTAQLQEDLYKEMRARIKETD
                     MSVPARYMDYYYYARTEEGRQYNIYCRKKGSLEAPEEIILDVNRLAENFDYYQVGAFE
                     ISPDQQLLAYTFDTAGSEVYTLVVKNMLTGDYFSDTLPNVSAVAWAGDNLTLFYTVED
                     ETRRPYQLFRHKLGAAPAEDRLVYHEQDPAFYVGVGETRSRRYLMMMIGSQITSEVWY
                     LRTDNPESDFTLFAPREQGVEYSVDHQGDNFYITTNKDAVNFRLLRTPTADPSPANWT
                     EVIPARDSVKLDGVDCFKDYLVVYEHDRGLPMVQIRTVSDGAVHYIAFDQPIYSVYPN
                     RNPSYDASKLRLNFQSLITPSTIFDYDMDSRKRTTLKQEEVLGGYNPEDYAQERVWAT
                     APDGTRIPISMCYRKGMVRDGSHPLYLYGYGAYGMTMEPWFSANRLSLIDRGFIYAIA
                     HIRGGGYLGRPWYEDGKMLRKRNTFTDFIACGDYLAEQGYTAPDRMVISGASAGGLLI
                     GAVVNMRPDLAAVAIADVPFVDVINTMLDESIPLTVIEYEEWGNPHDPAYYDYMMSYS
                     PYDNVTAKDYPAMLVTAGLNDPRVQYWEPAKWTAKLRATKTDDHVLLLKTNMGAGHGG
                     ASGRYDYLKEVALEYAFILDQLGLTGP"
     gene            complement(17037..17396)
                     /locus_tag="PKM94_05105"
     CDS             complement(17037..17396)
                     /locus_tag="PKM94_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66053.1"
                     /translation="MWTDVLIPLVIFGTGVVIVKLVLDYKLRRQILEKGPVDDKAQRF
                     LLREAELARLSSLKWGMVLVGLGIALLVGYMNPDMFDDGGVFGLMLVLAGIGFLIYFA
                     VAQRHLGRLERPPQGSV"
     gene            17528..18127
                     /locus_tag="PKM94_05110"
     CDS             17528..18127
                     /locus_tag="PKM94_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66054.1"
                     /translation="MEQAAPDNRSLIEEILRGSQAAFRTFVSRHQRLVSHIVFRMVAN
                     AADREDVCQDVFLKVFHNLGSFRHDARVSTWVARIAYNACIDHLQRRKIPLLEDLAGE
                     DGSSPLETIADPAEGADAAAETRDLFARVQAEMAYLPPQYRAILTLYHLDEMTYEEIV
                     EITGLPMGTVKNYLFRARRQLRDRLLTKYRTEDIWLTGT"
     gene            18109..18657
                     /locus_tag="PKM94_05115"
     CDS             18109..18657
                     /locus_tag="PKM94_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66055.1"
                     /translation="MVDRHMTDDEMQAYLETGTSALDQVFESHLAECRDCRERVESYR
                     ALFAALGRDPGWVLSPGFVGAVEARLAADRAAGAKRRWYHALWWLGGMAAAFGATAYF
                     MGLGTFTGYLRGVFEPSLQHGTRAFNNARQAVGTLEGVPQSVAGDGGNTLIVVGICLL
                     ILAAVAGADHLIFRSRQAKFCL"
     gene            complement(18716..20617)
                     /locus_tag="PKM94_05120"
     CDS             complement(18716..20617)
                     /locus_tag="PKM94_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HOD66056.1"
                     /translation="MQTVSPTPRRDALRAGSWFFLINAGFAILIATRYMVHFTAIAGL
                     GAYLYIAIVTLSHFVALVFIPWLALYAPVALLVPRRGVLLPWGALIAALTLGLLALDT
                     HVFNLYRFHINRFTLELLFGGAGAEIYEFHPSEYAFVATVLVLGILFELFLFRWVWRG
                     QWWRPLRVARWAVPAVLLMMLAGHAVHAWAAANGSRSITRASRFYPLYFPATANDFFI
                     KHGWASPEQNQLALAAGADESRKYLRYPLAPLRADCGSGANILLILLDSWNYRALDSL
                     VTPRLWHFAASAEQYRNHYSGSNGTRTGIFSLFYSLPGTYWYEVLAAKIRPVFINHLL
                     ACGYRFGVFTSASLVSPPFDQTVFAGVPGIPLRVPGERAHDRDLQITRDWLAFTEDYA
                     ASTGAPPFFGFLFYDALHSISHPDDFTGPFQPAWTHAKYSALGEDTDPAPFWNLYCNV
                     AYFEDSLVGLVLADLEAHGLLENTVVIITGDHGQEFNENGKGYWGHNGNYSRAQLGVP
                     LIIRWPDSLRPTDSLSAPAAQPPVARHWTSHFDIVPTLMQRLFRCQNPVGDYSIGRNL
                     ADAAGRGWLLVGSEDNFAVLEPDRITSINFDRTFDITDTSLNELPGASLNASLINDIL
                     RLSNAYYLK"
     gene            complement(20729..22891)
                     /locus_tag="PKM94_05125"
     CDS             complement(20729..22891)
                     /locus_tag="PKM94_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD66057.1"
                     /translation="MSAVRNRLRASALAALAGVCLASGVRAADPSGGWEGSIDIPGSA
                     LAFTVEFSRDSAGAWAAVVDIPAQNAADLPVEGIIVTDDSLIFALAGIPGAPVFRGAF
                     AADSGAVAGNFTQGGQAFPFSMTRLDVAARQARAAALADNLARIRSFIDTALAPWKVP
                     GLAIAIVKDDSVLMMEGFGLRDRENNLPVTPRTLFPIGSSTKAFTTTALAMLVDSGLV
                     EWDGRVRDYLPDFRLSDPEITDQATVRDLCTHRIGLPRHDLMWYSSDCTREEMYRRLR
                     YLDFSKDFRSEFQYQNLMYMTAGYLVGRVAGTSWEEFVRARIFLPLGMTNSNFSIAEL
                     EKAPEFARGYEEKNDTLALMPYRPIEAIGPAGAINSSVDQMVNWIRLHLANGLRDTVR
                     LVSEGQMIEMHSPCVSLDRAGGRYHETILTSYGLGWFIESYRGHYRVHHGGNIDGFSA
                     LASFLPDDRLGLVILTNKNGTPLPSIVANYVDDLLLGLEPVDYHRRALTQLAAADSSR
                     GTEAQAAADRVPNTKPSHDLSAYAGEYEHPGYGVVTVSLAGVPRKDQHLRAVLHSLES
                     DLEHWHYDVFRMIDEPLADKKARSFLSFSTNTFGDIDRLSVVLEPTLPPIEFVRRPDS
                     RLSEPAYLAQFTGDYLLEQLAVTVAIQADRLTVTVPGQPTYTLLPYRNDEFTFRDLSG
                     YSVAFDRAKDGRITGLRFKQPNGVFSAEKRTAEDTQNK"
     gene            complement(23123..24535)
                     /gene="glnA"
                     /locus_tag="PKM94_05130"
     CDS             complement(23123..24535)
                     /gene="glnA"
                     /locus_tag="PKM94_05130"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOD66058.1"
                     /translation="MNLAELQKLAESHRAEFLDLKFCDLLGVWHHMTLPIAALKPSLF
                     EQGVGVDGSSLPGFSSIERGDMILIPDTATAFMDPFFDRPTLSMIGDVMESADRILPY
                     SRNPRRVAADAERFLQAAFPGVQCILGPEFEFYVFDKVNFFQGAESAFYHLDSVEADW
                     SAAADEESLGFKIPHKKGYHIAPPMDRTFNLRSELAVLLADVGVHLKYHHHEVGSGGQ
                     HEIEVEFSPLRRMADLSMLVKYIIKNHCFRRGKSATFMPKPLFNEPGSGLHVHQYLAD
                     ANGSLFYDKNGPAMLSETGLHFVGGLLKHVDALLAFTNPSTNSFKRLVPGFEAPIIGS
                     YSMGNRTACIRIPGYQRDPRKMRFEFRPPDGTMNFYLAYAAMLMAGLDGIKRKLDPGA
                     PLDRNADHLTPEELKAHHQLPTTLTGALDALENDCDFLLQGGVFTRDLVENWIALKRK
                     EVAQIQIRPTPHEFELYYNT"
     gene            complement(24706..27204)
                     /locus_tag="PKM94_05135"
     CDS             complement(24706..27204)
                     /locus_tag="PKM94_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66059.1"
                     /translation="MASRSPAYCMTLHNVGRLVLAVNNNGTFGSEFSVPSDAPDCFTG
                     QTPPPGGCQYPKGTGAMYMFSAAFWIGAVLGRDTLVSTGSEGWSDVRQFFPNDEPFGN
                     TVRRSIIDPESEEYVNAVSEQDFIALYTDTSTILADAEEDGTAHRPLNIQVTQRSYAW
                     SYDYADDIILFDYEIKNIGNIDLRNVYMGVYVDADVCDNCQDNEGFADDICGFLVTYP
                     REYGRMRCEYNDTVYIAWIADNDGDFNAGEGIQSTPHVTGTRIVRTPADTLDVSFNWW
                     VSNGNAALDFGPREKDSVGRWKEPYRDFRTGGTGTPVGDRNRYYSLRNREFDYDQIFT
                     NTIREGDPSWIKPPSALAPDIADGFDTRYLLSFGPFNIRKGQKLPLSFAYVAGADLHR
                     DPDNGDNLLADPVLYYSNLYFDSLAENATWASWIYDNPGVDSDSDGYSGKFRCMATLP
                     DGSRICLEDCDTTAANAPGAQWEVVAYMGDGIPDFRGARPPDAPRIWLEPKVGQIRVR
                     FNGKASETGVDRFSGLNDFEGYRIYLGRDNRETSFSVLASYDRHDFNKWDYDLDKGWQ
                     LNDPPYTLDSLRALFQDPTLDPGAWTFSRAYTPAVNPDPRFPQYYFAAQDYNVSDLTN
                     LKGIHKIYPDQPYPSSLIPSEADPSELTPDGFFKYFEYEYIIDSLLPTVPYYVNVTAF
                     DYGSPSSGLSSLETSKTVGSKMAYPLATAQAVEQENLDVIVYPNPYRSDGGYRALGFE
                     GRGADDINRPDSRVRDLHFANLPAKCTIRIYTLDGDLVREIKHDMDPSDPNASHDSWD
                     LITRNTQMVVSGLYYWTVEDEEGNTQMGKVAIIM"
     gene            27737..28636
                     /locus_tag="PKM94_05140"
     CDS             27737..28636
                     /locus_tag="PKM94_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD66060.1"
                     /translation="MTEFPVSGVETWWWLPSLVAFCVSTLTSTGGLSGAFLMLPFQVS
                     VLGFTGPGVTPTNLLYNVVGIPSGVYRYGREKRMLWPLAWAIVIGTMPGMAIGTVIRV
                     TLLPDPSAFKVFAGLVLAFVGVRLLRDVVRGEGARPSPRGNGAGRFEVSGGRLTKLTV
                     EYDFGRQRYTASTPKILLLSFLVGVAGGAYGIGGGAVIAPFLVSAFGLPVYTVAGAAL
                     FGTFVSSAAGVAMFWLIDLSGLTGSTAAQPDWLLGLALGIGGAFGTYTGARLQKRVPA
                     RGIKALLTAFLLFVAGRYIVEAF"
     gene            28654..29094
                     /locus_tag="PKM94_05145"
     CDS             28654..29094
                     /locus_tag="PKM94_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD66061.1"
                     /translation="MDDTIAKFASPAPIAVVGATDETRKFGGYLYRELKRQGYRVYPV
                     NARRAEVDGDRAYRSLRDIPDDVEGAVVAIRPDRAGEVVADALARGIRRIWFQQGADF
                     TEAARTAREAGMETVVGKCILMYAGKARGVHRFHAVLARLFGRY"
     gene            29115..30371
                     /locus_tag="PKM94_05150"
     CDS             29115..30371
                     /locus_tag="PKM94_05150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66062.1"
                     /translation="MATEIREIEDRDLDAFIRLQIDAYPGLGMIYERDREKLRQRIAE
                     RRQDVRVTLYGSYRGNRLVGCMRVHDYRMNFRGALVAAGGLGGVAVDLTVKREGVAGG
                     MVRFYLDKYAAAGAPMAVLWPFRPDFYRKMGFGYGGKKYRYTILPETLPHAAGKGHLA
                     HGEERDFAAMTRCHNRLLALRTGMLDETELSLRQTVGAAGGGRAVVYRDGREVRGFMV
                     YRFRPGAPGNFVSNDLCVEECLYESPEVLAEFLAFLHSQRDQVRRVILDLPDPNFHYA
                     PVDPRNGSEGLYASVYHEVAACAVGPMYRVLDVQSLLAAASEAKFGPGEATVAFTLAD
                     RWWGKTAGTTAIHFCEGRAAAAPKRKCDVAVEMEVADFSSVILGAVRLSQLVDYGLAR
                     VSKRKSAEVVDSILAAAQPPYCFSRF"
     gene            complement(30440..31744)
                     /locus_tag="PKM94_05155"
     CDS             complement(30440..31744)
                     /locus_tag="PKM94_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD66063.1"
                     /translation="MSKCLAFACLYLCTASAAFGAPETGWETFRPDRQVQFDRLGRST
                     DTCLLFRHNPDDSVLGYSSSFTAGQQTIVYFDPTSCAADSTYPFGITELSFTLLDPPS
                     YIDSRLYKWPVELEVVVYALAPSGDPCDGPGALLCSVSAACDSASFAFPQVGTVPLPS
                     PCCFDAPFFIGIRYTDTTSLRLPSIMWDTDSSPALCDAFQFYDTAWYAWNIFWVYDPG
                     FPFFWVGGEARSLACCDDSDGDLICAQYDNCPAVANAGQLDTDGDGLGDACDNCPNDP
                     NPGQEDADADGYADACDNCPAVSNASQADADSDSLGDLCDPCPTDPSNDADGDGICGA
                     ADNCPGHYNPLQEDSDADGTGDACEVLDICTGIRGNIDGYLNDLVNVADITFFVAYLF
                     RGGPAPPVMAEADVNADGEIGIADLTFLVAYLFRGGPAPAPC"
BASE COUNT         5713 a         9876 c        10931 g         5459 t
ORIGIN      
        1 agaccgatca taaactacat caacacacaa aattgtcaag aactttcttc aacaaaatac
       61 acaaatcact aaatacggtg agatgtagta ataaaacctc gcaatcacct aagcgagctc
      121 tcgccccggc gcctacccca gagccgaact agtatcgcgg ccccgaccgt caaattggac
      181 cgagcaaaac cggcgtaccc aaccgctagc gacagccgac cgggctcggg tatctatacg
      241 cctggtattc tggcgaacct ggcgccaccg aactgaccgg acggacaaag gcggggtgaa
      301 ggcctcagag cggaagcatg gggcgccctc ctcgcgaggc agattgtgac gatagtcaca
      361 atctgcccgg taacgaccct ctgcgaggca gggtcgatca tagtgttgcc catccatgaa
      421 atcaccgcct gacgtcggcc ccactattgg cgcccgcccc ctaaaagcga gggggaccga
      481 cgcctgctgt tgcctcattt cgcccgtccg ggtcggcccc gccgagccgg accgtttcgc
      541 ctcttggggt cgccgtgata cggttaagac atcctcgctg ccggagtcgc ccgccgaagg
      601 gcgaccctaa cctgagcgac ctcccggtac cggcatcggg cagcccggca ccaccccttt
      661 gatgatcctc tcatgatggc cgggagttgc cccctcacgg agatgcctcg gggaatgact
      721 gtggataaaa tttatctgac cggctcgcga cacttcgatc cgcggcctct taaggtcccg
      781 actgtgtcat agatgacacg ccggctgagg gtcagaaaga gccccgtcat tggttgctca
      841 agagccaatg acggggccgc aaatggccga tagaagatcc ggacacccgg tcaccgggcc
      901 cggtatttct ggtagagctc ggtgtgtttc gaactcaaat ccaccgcccg gccgtcgatg
      961 aactccatcc gaacgcgagc ccgaagcggg tccagaaggt cgccgtcgga caccaccagc
     1021 gtggcgcgct tcccgacctc cagggagccg agctcgccct cgatccccag aatctcagcc
     1081 gaggtcagcg tgagggaccg gagagccgcc tcccacggca gtccgaagcc gaccgcctgg
     1141 ccggcctgga aggggagatt ccggaccccg gtcgccccgc ccccggccga aaaacagaac
     1201 tccaccccgg cctggtgaag gagggcgggg attttgtagt tgaggtcgta gtcgtcgtcc
     1261 tcccgcatcg ggaggcgctg cgtcggtccg aatataaccg ggatgccgtt gtcccggagc
     1321 aggggggcca tcttccaggc ctcccgtccg cccacgagga tcatccggaa accgtactcc
     1381 cggcagaggg ccaccgcctg atcgatctgc cggtagtcat cggcatgcac aaagagcggc
     1441 atcgtgcggt cgaacagggg gaggaagcct tcccagcggg aatcgacggg gatggcataa
     1501 tcggccgttt tggcgagata gtaggcgcgg gcctggtcga ataccccgcg gagctgcgcc
     1561 tgctcctcct gtcgggcttt tttctgctcc tcgaccgact cttccatcca ccactccgaa
     1621 gtggtcgacg cccgcggcca gctcaggtgg agcccaacga ccggccgcac cagcgcgtcc
     1681 tcgactgtcc agccgtccag attgaccaaa ctggaccgtc ccatcacgag ttggccgccg
     1741 ggcacgatca aggccgtggt gatgccgttg gcgcgaacgg tcgggatgat ctcggagtcc
     1801 gggttatacg ccgcgtaggc tttcagttcg cctttggcca ggccccgctc ctgcgtatcc
     1861 tcggtggcgc ggaccgcgcc gatttcggac agaccgagag tcgagtgcgg ggcgatcagg
     1921 ccggggtaga cgtgcagccc ggtgacgtcg atgacctggg cgccctccgg cgcttcgagg
     1981 ttccgcccga tggccgtgat gcgcccggat tcgaacaaca ggtccgcccc gtccatgacg
     2041 ccgttcgcca ccgtgaagag cttgccgttc cggagcagaa tcgggcgatc ctgcggcggg
     2101 ccggggagat agtcgtgggc ggctccgctg acagcaaagg cgacgagcat ggcggccgca
     2161 acacccgtcg tccgcagccg ctcccggaga ggtctaccgg tgtgcttcac gggcgcctcc
     2221 ttctgtcgaa gataacccgc cgtcgggcgg cggacccggc cgtccgcgcg gcggccgctt
     2281 gggcgacccg cctccggaag cccgcagtac tttctgaatc aaccgctgct tctctgtctg
     2341 aagctgctcc cggagcaggc tgtcggtggc caggtcgaag aacttccgac cgtccaccca
     2401 cgtctgctcc gcccgggcat atatggagag ggggttgtcg ctccagatga caaagtcggc
     2461 gtccttgccc acccgcaggc ttccggtcaa gtcatccaca ccgaggagcc gggccggatt
     2521 gatcgtgacc attttgagcg cctcttccgg cttcatcccg ccgtacatga ccgatttggc
     2581 cgcctcctgg ttcaggtggc gggccagctc cgggctgtcg gagttgatcg ccaccaccac
     2641 gcccttttcg ctcatgaggg cgggggagtg ggggattccg tcataggtct cgaacttgta
     2701 tgcccaccag tcggggatgc tgcccacgcc gacgccggcg tcggccagct cgcgggcgat
     2761 cttgtagccc tcgagaccat gggcgaaaga ctggagttcg aggtcaaact cctgggcgag
     2821 gcggatcatc atgagcatct ccggcgccac gtatccgtga cagtggatcg ccatccgccg
     2881 gtggagaacg tcggcgaggg gctcaagctg caagtcgcgg cgcgggggaa tggttcgctc
     2941 ctgctcgctc ttgccgaggc ggcggaagcg gtcccagcgc tgctcgtagg cctgggcggc
     3001 cagaaacgca tcgcgtatga tcgcgtccac gcccagcctt gtctgcggat agcggatggt
     3061 gaagcgctcc ccccagccgc tctgtttgac gttttcgccg agggcgaact taattgcctt
     3121 cggcgcggcc gcgaacacca ggctgtccgc cgggagcccc cagcgcagct tgatggtggc
     3181 cgcctgtccg ccgatcgggt tggccgatcc gtggagcagg cgcgaggccg tcacgccgcc
     3241 ggccagttcg cggtagatgt tcaggtcgtc cggatcgagg atgtcggcga tcctcacctc
     3301 ggaggtcacg gcatgggttc cctcgttgac gtcgccgtca attgcgatgt gcgagtgttc
     3361 gtcgataatc cccggcgaga cgtgtttccc ggcggcatcg atcaccgccg cccccgaggg
     3421 cgactcgagg ttgcggccga gcgcggcgat cttgccgccc cgcaccagca tgtcggcgcc
     3481 ctcaagtacg ccggcctcat ccgacgtcca gatggtcgcg ttgcgcacga gcacgtcctg
     3541 cgggaccggc accgattcca gaccgtaggc ccggttgggg taggtcaggc gggacacaaa
     3601 cggctccggt tcgggcgcgg gggacggcgc cccggcggtg tctttttccg ctgctagcgg
     3661 ggcggtgcgg accgcctgcc attcgctgcg ccggccgtcc gggaaggcca cgaatccccg
     3721 aagcgagtct ccgaacagcc gcccagagaa ccggcagact ccgggcttcc cccagaggct
     3781 gtcggcccgg aacgtaatgc ggtcgcgggc ggcttcaatc tcgcccagct ccacggaatc
     3841 ggagccggcc accagtcggc ccgcgagttt cgatggtgca ccttccaggg cgagtgtcgc
     3901 gctgtccgcc gcagttcgca gttgccactg accccggtat tcccccggat gcagcggctc
     3961 gaactcgtat ttctggccgg ccgcccagac ggccaggatg cgcgtcgtgt cggcgaacag
     4021 gtcgccgctg cacacaacga ggttggccag cttccccggt tcgagggtac cgacataccg
     4081 gtctactccg cagagccgcg cagggaccgt cgtaagcgct ctcagggccg tttccggggg
     4141 cagaccgtac cgcacggccg tgcggacgcg ggagaggtat tcggaggggt cgtccaagcc
     4201 ggccgcggtg aaagcaaacg ggatcttttc ctgggccagg cgcgcggcgt tgccgggggc
     4261 gcgctcccag tgccggagat cctccagggt gacctcgagt tcgtcctcca gagagcccac
     4321 ttccggcgct tccgggtagt tgaccggaat gatcagctcc accagctcac cgcggatttc
     4381 ctcagcccgc tcgtattcgc acccgctgcc gacacaaatg aaggtccgcc cgaactcccg
     4441 ggcaagccgt gcggcccgga tgatttcttt gtcatcggcc gccacgaaaa tcatggtgtc
     4501 agcctgccgt cccagagcct cgatggccgc gttgaattcg ggcatttcct gaccggggtt
     4561 gcgttcccag gccgcccgcg cggcccgata ccagtctacg tcgagcaatg tttgccgaat
     4621 cagggcgata gcccccattt gcgaagaggg gtaatcctgc gccgatgagc ccttgtagaa
     4681 cgacagcgcc ggataggagc gggcccgcag cacctgattg ttgggcagcc cctgccgcag
     4741 gagaaccaca gccgggcgtc cccggaagat tccctcctgt cgggtcgttt gcgtcaccgt
     4801 gatgcccgcc ccgaggtact tctgggccgc cgtcgagtcg ggtcggaagg aatcgaccca
     4861 atcgatctcg gcgcggatcg cctcgttcca ggcgctgccg ccgatccggt cagcctcata
     4921 cttgggaccg ccccaggcga ttcggccgcc ctggccgtgt ccctcttcct cggcgagcag
     4981 gccgtagtcg gagtggggat cgatgaatcc ggggtagacc acgcggccgc cgaggtcaac
     5041 caccagcgct ccctcgggta cgcgcgccga ccggctgacc tcgcggatga caccgtgctc
     5101 gaacacgatc accgccgaat cgatcgtccg gtcgggggag atgacgacgc gggcgttgac
     5161 cagggctgtg agatccggcg tatgatcgcg aatgcccagc tccggggtgg tgccggaggc
     5221 ggcgagcacg ctgccggcac acagcgcgag aattatgacc gagtgatttg acttcatgat
     5281 gatgctccca gttgcctcga cctgcagtac ggcgaaccgc ctgcgccaga gttaggatgt
     5341 cgcggcaata tgtcgggaaa cacggccgat ggcaagcggc aaacggttcc gacggcccgc
     5401 gtgcgaaaac gccgccggcg gcggccggcg gcgcgaagtc tgttgcagtg gaggaccggc
     5461 gatcccggcc cccgcgcctc tatgggcagt cggccggcgg ggggccgccc tggaagaggt
     5521 aggcggtcag cgccgtcagg tcggacactc cgatggttcc ggaggaatcc ccgttgatgt
     5581 tggcctcctc aagacagccc ggcgtcggcc cgccctggaa aaggaacgag atcaggaatg
     5641 tcacatcggc aatggagatg ttgtcggccg cgtcgccgtt cacgttgcct cgccgggggg
     5701 ccacgcagca cccggcgtcg cacgcgtcgc cgatgccgtt gctgtcggca tcggcctgcg
     5761 acggattata ggtgtacggg cagttgtccg tcgggcaggt gttggccggg tacccggggt
     5821 tgccgtaacc gtcgccgtcc gtatccgtgc acggatcgca ggcgttgccg aggccgtcgg
     5881 cgtcaatgtc ggcctggtcg ggattgccca ccgtcgggca gttgtcgcag gcgttaccaa
     5941 ggccgtcgct gtcgtcgtcg gcctgcccgg cgttggcgcg cgccgggcag ttgtcgcagc
     6001 tgtccggcac gccgtcggag tcgccgtcct gggcatcgtc aaacccgggg cacaggtcgc
     6061 acccgtcggc gacgccgtcg ccgtccgtat cgacgttgtc gttaaagccg ggacagacat
     6121 cgcagccgtc gggcaccccg tcggcatcgg catcgaggct gtcgtcgaat ccggggcaga
     6181 cgtcgcagag gtcgcccgcg ccgtcgccgt cggcatccgc ctggtccggg ttggcgacca
     6241 gcgggcagtt gtccgtgggg cactcgtttt cggggtggcc gggatcgccg aacccgtcgc
     6301 tgtcagtgtc cacgcatatc cattcgtcga tcgccagccg caggacgaac atgtcgtacc
     6361 cgccgccgtg ggtcccctgg atggcgttga cggtcggaaa cgtcgtgctg ctggtatacc
     6421 cgccgatgta ggcattctgc ccggtgtcga ccgccaccgc gtagccgaaa tccagcgccc
     6481 cggagccgag gaaagtcgag tacacgaggg tgtcgccggg ctggccgaaa caggtcgcga
     6541 aagcatcggc gccggccgcg aacgtgctgt caacggccgc gcgcgtggga aaattcgggg
     6601 agaaggtgtt gcccgtgaca taggcgaacc ccaggtagtc gacgtcaatc cccgccccga
     6661 gatcgttccc gctcccgccg aggtaggtgc tgtaggcgag gctgttcccg gacgggccga
     6721 gcttggacgc gaaagcgtcc agataggcgt tgtgcgtcgc gtcgaagggc gcgacgacgg
     6781 ggaaatccgt cgagagggtg tagccggtga caaaggcatt gtccgccgag tcgagggcga
     6841 tggccaggcc ggcgtcatcc gccgctccgc ccaaaaacgt gctgtacacc agcccgctgc
     6901 cggcggcatt gaaacgcgtc acaaacacat cgccggccga ggatccgccg gcgttcgtgg
     6961 cgtcgtaggc cgagagaacc gggaaatctg tcgacagcgt gtagccggtc acgaatgctt
     7021 cgcccgtacc gttcactgct gtcgccagcg ccgcgtcgtt ggcgctgccg cccagaaacg
     7081 tgctgtacac ccgggggccc gtcggcgaga acttgcccag aaacgcctcc tgcgtgccgc
     7141 cgaacacgtt gtcaaagggc gacccggaga gggggaaatc ggatgactcg gttcgcccca
     7201 cgacatacag attgccggag ttgtccacag caacgccgtt ggccacgtcc gaggcggttc
     7261 ccccgaggta cgacgccatc agaagggcgt cgccgcttgc cgacagccgc agcagcgcgg
     7321 cgtcgtcgcc gcccgcgagc gccccctggg aggcgctcac gaccgggaaa tcgctcgagg
     7381 acgtctgtcc gaccgcgcag gcctcgccgg cgccgttgac cgcgacaccg taggcccggt
     7441 catcccccgt ggccccgccg atgaaagtgc tgtaaatcag cgagtccccc agcggcgaaa
     7501 tcttcgtcac gaccatgtcg taaccgaatg tccccccgcc gttgtagctg ccgtcgtacg
     7561 cggccttgac ggggaaatca accgacgagg tgtacccggc cgcgtaggca ttgccgtcgg
     7621 cgtcgatcgc gagactccgg cagtaatcgt tcgatgcgcc cccgagcatc gtggaatact
     7681 cgaccaccgg gtcaatgaca agggggaggt ccgggttgta accggccggc agccggaacc
     7741 caaaggtgtt gtctccgaga attacaaatt gggccggcac ggccagccgc gacgatccgg
     7801 agagctggta gacgaccggc gcgtactctt cgatccggcc cccgtcggtt tcgattacca
     7861 gacggccgtc ggcggcgatc accgtccgtt ccgcgccctg caccgcgata cggatgcggg
     7921 acggatcggc atgcgggtcg atgtggaaat catactccag agcgtttcct gcggcgcggt
     7981 acaccagatc gatgccgtcg tacaagtctt caaatgcaac cgccccgaat tgcggcacgt
     8041 gggtgcgcca gtctgcggga tccgcgccca cgaggtagtg gcgcaggccc ggcattggct
     8101 cgcgcccgac cacggccggc gccggattcg ccccggcgaa agagaccctc accaatcggt
     8161 aggtgaaacg cgcggcttcg ccggccgggt aatacccgta gcgggagaga tcgtccggag
     8221 gcggcgttcc ctccgcgggt tcgatgaatt catagaatac ttcgtttccg gcaacccaga
     8281 caatggcatc gccgttctcg gcccgaaacg tcaccggcgc ggcgtactgg cccacgttgg
     8341 ggacgaacgc cagggaagcg gccgcgagtg tgattccgat cggcaggtcg gcggaacgat
     8401 cgggcagcgg cgcggacgcg tacagtccac cggttgcggc ggccgcgagc gcaaccacat
     8461 atcccgataa gactgtcagt gtgaatttgg ccctccgggc caaaaaccgg cagtttgtca
     8521 cttgttactc cggatgtcga tctgcaacga aactacaaag aaagagatgt caatgcactt
     8581 acacttccaa taaacgccgc gaccctccgc ttgtcaagtc caaatccgga atcggccgca
     8641 ggcccaacac atatgcctag attcgacaga ctccgcgccg gggtttatcg ttgccggccc
     8701 gcttttttcc gaggcgtccc tgtatacgca gtaatacgga atggacgccg cgctcccgaa
     8761 ggtctattgc gcacgatgcc ggccgttttc gcaaatcggc gggggtccgc cccgaaacaa
     8821 aaatccgata aaataggtca ggtcgcctac cgtcggaagt cccccggaac cgtcgatgtc
     8881 cgcggctgcc atgtccgccg gcggcgggcc gccgcggaaa aggaaattga cgaaataggt
     8941 aaggtccgcc acgttcggcg tcccgccgtt cccggtgagg tccccgcaca cgtactgggg
     9001 ggggagcgtc acgtgcaccg tcgccggcac cgaccacggg ctctcgtcgg gatcgttgct
     9061 ggtcaccagc aggatcaggc cgtagctgcc ggagccaaga ttcgccgtcg tgattgtcag
     9121 ggcgagcgtg tcggccccgc ccgggtcgac cgacccggcc gtccgctcca gcgccagcca
     9181 ctgggaggga ttggtgaaga gaatggccag cgagtctttc agatagggcg tcaggtaggc
     9241 cacctccagc ccgtcggcgc ccgcggggtt ctcaatcccc accgcgcagc tggcaacgtc
     9301 gaaacccggc gcgatcgaca ggtactggta gacgatcgac ccgtccggat acaggatcac
     9361 ttcagcgttc accacatccc cggcgccggc caggtactcc ggatacgcgg agaactggat
     9421 gacgcagcgg gccggcgtcg cgtcgacata cacggccgcc cccgggttgg ccccgtcggt
     9481 gggattcaga tcatcccata gccaggccag cagggcgttc ggcgcggtag tgtcggggag
     9541 gtgcctcttg aaccggctgt tcagacttat cggtgcgaac ccgatgattc cgttcgatcc
     9601 cacaaagagc gtcgagtacg tgctgtcgta gaaggggaaa tcgaagccga tcgcatacgg
     9661 cccgctgaac gaatcatcgg tcagcgtgcc gatgatgttg atcccggtgg tgcttgcctc
     9721 aatccaggca aaggtcggcc cggccggctc atcggagtcg acccagaaat acccgtaggc
     9781 gtccgggcct ccggcatcac gcgtcaccgg cgctccgatc cggggatcct cgccgccctt
     9841 gggctcggcg tagtcgtcga acccatccgg ataggaccgc agcgccggct ccaggccatc
     9901 ttctgctgcc aggcgcgcga acggcaacgt ggccgtcgcg gggacccggc tgagcgagta
     9961 gtccagcccg cccagcccct cattttcgat aatcaggtgc gcggccgccg aatccccctg
    10021 ctcgagcgtc acctcaatgg cggccggggc cacggcgatg tccggggcgt aatcgatgcg
    10081 gatatcgtca atgtaccagt ggtcgcagtt ggcgcaggaa ccgaacgagc gcagccgcag
    10141 ctggaacccg ctgtgcaggg ccgccgggac cagggggatc acccgctgtg cgaagtagtt
    10201 catggcgccc ccgccccccg ccagttgatc gagctgcacc cactgcccga ccgatgtctt
    10261 gtactccagc cacaaatcct cgcccgcttc cggcgggtcg ctcgccgccc gctggtaata
    10321 gaaggagaga ttcgcctcgc cgaccgcgct caggttgagc acctggctga caaccgtgtc
    10381 gatcccgccg tcgaggtcaa gcgcgtacgg cggggtcggg ggatccaccg ccgacgcggt
    10441 gaccgtggcc cccgaattct gcggccaccg gaaggcgatg agggtatcct cggggaagct
    10501 ctcgtaccac gggaatccgc cgggcggtcc gctcgagacc gtttcaaggg cggcggtgtc
    10561 ggctaccgac gtgttgctca gcgagaccac gcgcacccgg gcggcgctcg cgtcgttggc
    10621 ggccgcgtcg gccgggatgg gcactttcgc ggcaaaccgg ctcgagtctc cgctggggac
    10681 gaaatcggtc acggtaataa tcccctggcc gagcgaatcg taggccgcga ccggccaccc
    10741 gccgagatcc tcggcgaaca ggtggtagcg gtccgtgttg agcccccggt tgtgcaccgt
    10801 caccaagtac attaccgtgt cgccggcgaa cccgtactgc cgggactgcg gcggtgtcac
    10861 gcgaacggcg taggccggcg gcggcccgac atacacgtcg tcgacaaacc agtcgtcgaa
    10921 cacgccgatt gtgcccgtgc agcgcaggcg cagacggaag cgccggtgga aggcgtccgc
    10981 gggtagcgaa aactccacct cctcgaacgt cgtcatgtcg ggatcggcgc cgaggtgctg
    11041 gcggatcagg cgccacgtgc cggtcgaatc gaaatactcg accacgagat cgtcgccggc
    11101 ttccggcgaa tctccgcccc cggtccgctg gtaagcgtac ccaaccatgg cggccgtctg
    11161 cccccgcaga tcgatcgcca tcgagagtac cgtatccgcg ccgtaccggt tgccgtcgag
    11221 gttgaggctg tagggcggcg agggttccgc gagcccggcc tcgctgatct cggcttcgac
    11281 cacccccgcc cacctggagg aatcgaacgc ggcggcggcg aattcctcgc tgaacggaac
    11341 ggcgagggcc ggtccgtccg acaccgtcac cgcggcgccg gcagcggtca ccgccggatt
    11401 cgccgtcgag aggacggcca ttaccgccgt gtccgccgcc agataagtgc tggcggggat
    11461 gagcacgcgg accttgactt tcaccgaatc ttccgccccc accgccccgg tcgcgctgat
    11521 cgggccggcc atggtgctgt cgaaaatctg ggtcacccac ttcgcctgtc cgatccccag
    11581 ggtgtagtcg tccgcgcgca gccccgtgtt gaccacccag aggtcaaacc acatcgtgtc
    11641 ggacaccagc cccgtctgcg tctgccagtc ggggcgcaca ctcaccgcgt agtcgatcac
    11701 ccggaacgtg aagatgtcgg tggtgtccgc ttttccctcc acgtccgccg cggtcagata
    11761 gtagcggatc gtcgtgccgg gcggctgggc gggaatggcg ccgctgtact ggactccgcc
    11821 ggccggggaa aggatcgcgc ttgtccacac gctcgcaatc tcgtagtgca ggcgcagcga
    11881 atccggcgcc agagtcgtgt ccgacgtaat ctgggcggcg accgcgtaat cgttcacggt
    11941 atcccgggtg tccgcgagcg gttggtggac gatcgtcacg ccggtcggga tggcctgcac
    12001 cagcaccgcc gcggtgcgaa ggttgagccg tccgcccgtg accgtcacgc cggtgagcga
    12061 cccgatcgag tcgacgccgt ccaagatagc ctggcggata agcagggcta ccgagtcggg
    12121 gcgccgccgc gccaggtcga tgaccacggg ggaggctgcg ctgtacatga acccgactgc
    12181 ccccgcgaca tggggggagg ccatcgacgt ccccgtcttg ttcccgtacc cgcctccgag
    12241 cagcgtggag tagaccgagg tcccgggtgc gcccaggtcg atcgaggcgg cgccccagcc
    12301 ggccccgctg ttgcgcacgt cgctggaggt tgtgttggtc accgcaatca ggtagtcgct
    12361 ggggcaggcg gtggggacgt cgccgtttgc gtcgatgtcc atgccgatgt tggcggtggc
    12421 cgccgcgctc aacacgccgt aggccccgag cgagtcaaac atggcgcacc agacagggaa
    12481 attgaccggg tcgccgtagt ccacgccgaa cgaggaattg gtcgccacca cgaacgctcc
    12541 gagcgcgccc tccgattcgt tgtactgcgc ccgcatctcc agggcgtagg cgtacgcggc
    12601 caccaccgtc gcctcggaag tcgaggaccc ggcgatcggc atcaccttca cctgccagtt
    12661 gaccccgctc acccccgcgc cgttgttgcc gatggccgcg gcgatccccg ccacgtgcgt
    12721 tccgtggtcg ctcgccggca gcgtcccgtc gttcgagtac gcatcccagc cgtcgtagtc
    12781 gtcgatatac ccgttgccgt cgtcgtcaat cccgttcgcg ggcgtttcat gggtgttctt
    12841 gaaaaaggag atgtcggggt ggttcaggtc gtacccgcgg tcgacaatgg cgatcacaat
    12901 ctgatccccg tcggccgtcg tcccggaggt cgtcagatcc caggcctcgg gagcgtcaat
    12961 atcggcatcc gcaacccctc ccgtctggcc ggtgttgtgc agcgcccact gctggccgaa
    13021 cgaggagtcg tcggggaagg tcgagcggag ggccacttca tgattgaact ggccgagctc
    13081 gaccgcggga tgccgccgca cccgggcgag cacctcctca tgcgcagccg tgtccttgtg
    13141 cccgtcggcg cggtattcgt agagccagat gttcaggcgg cgcgagagca gttgccgggg
    13201 ccgcaggtcg agggcggcga aatccgaagc cagttggccg gcggctttgg ccgaggacaa
    13261 ctgcagaatc atctctcccg gcacaaagac cggttcccgg gtttctgcct ccgccgtccg
    13321 agcggcggag gccaggatga gaatgaccag gatgagggtc gccgagaggg ggaagtgccg
    13381 gcctgtgtgc gcgcgcatgg aatacctcag agtcgggtgt gacgatttgc ccgtccggcc
    13441 acatcggcaa cgcggtcgtt ggcggtcgtt cggcggatgc ggtccgacgt acttctccgg
    13501 gtcggtacta cactacaatc tacgtgcttg ccggtcgatg tcaacacctt tgaccggcgg
    13561 cgcgggcggc aataatggat tgccgccgcc gacggcggcc ggtcgcccgg gggggaggtc
    13621 ctcccgggat tgccgcggct cccggccgca cagtttctgt cgctcgccgt ctgcttcctg
    13681 ccgtacctct tcctcatcaa tccctcctga tgatcggagc gccgcatgtg gagccgttgg
    13741 aaaatgcgca tcggccttat tcttggtccg gctgcggccc cgggcctcct ggcgccggcc
    13801 gatctccagc ccggccaccc ggccgtcacc gccgccgccg cggtcgctgt cctgatggcc
    13861 gtctggtgga tcacggaggc cattcccatt ccggccggcg cccttctgcc gatcgccctg
    13921 catccggtcc tggggatcat gaagggggac gccgtggccg ccaagtacgt cagcagcgtc
    13981 atcttcctct ttatcggcgg attcatcgtg gcccttgcca tggagcggtg cggtctccac
    14041 cggcgcatcg ccctgcgcat catcaccctc atcggcgcct ccccccgctg cctgctggcc
    14101 ggcttcatag ccgccacctt tgttctgtcg atgtggattt ccaacccggc ggcgacgatg
    14161 atgatgattc cgattgcgat ggccattgtg ctgaaggtgg aggaaggcgc accgggcgct
    14221 ccgaccgcca ggttcgccgc cgttgtctgg gccgtcctca ccgccttgta tgcaccgtcc
    14281 gccggcgagc taaaggaggg aaaggaaatc tttgcgtggt tgggatctct acgttggctc
    14341 ggccggcgcc gcggggaggg ccaacgagaa atcagggtgg aaaaagcacg ggaccggttc
    14401 ggcttctgta acctgctgta cgccaatacg gaaggctctt gcgaagaggc ggcggcgaac
    14461 gcatggaacc cagtttctcc gtgacagtct gccgtcgagc gtataaattg gagctttcat
    14521 gctgtgtgtt caacctcagg aggtttcggt tcgatgaagc tctcacactc gctggctctc
    14581 attttgatcg cggtcgccct tgtcgcctcg gccgcgttgg cccaggaaac ggccgtcgca
    14641 ccgcatgccc cggtagccgc catgatcccg cgcgtcgaca ctgtgaacgg caatatcgtc
    14701 cgcattgacg actactactg gctccgcaac cgcggggact cggctgttat ggcgtacctc
    14761 gaagccgaga atgcctacac tgaggcctgc atggcgcaca ccgcgcagct ccaggaggac
    14821 ctctacaagg agatgcgcgc ccgcatcaag gaaaccgaca tgagcgtgcc ggcccgctac
    14881 atggactact actactacgc ccggacagag gaaggccggc agtacaacat ctactgccgc
    14941 aagaaaggct cgctggaggc tccggaggag atcattctcg atgtgaacag gctggccgag
    15001 aatttcgact actatcaggt cggcgctttc gagatcagcc ccgatcagca actgctggcc
    15061 tacaccttcg ataccgccgg cagcgaggtg tacacgctgg tggtcaagaa tatgctcacc
    15121 ggcgattact tcagcgacac cctccccaat gtctcggctg tcgcctgggc cggcgacaac
    15181 ctcacgctat tctacacggt cgaggacgaa acccggcggc cctaccagct cttccgccac
    15241 aagctgggcg ccgcgccggc cgaggaccgg ctggtctatc acgaacagga tccggccttc
    15301 tacgtcggcg tgggggagac ccgcagccgg cggtacctga tgatgatgat cggcagtcag
    15361 atcacgtccg aggtctggta tctgcggacc gacaatcccg agagcgactt cacgctcttt
    15421 gcgccccgcg agcaaggggt ggaatattcc gtcgaccacc agggggacaa tttctacatc
    15481 acgacgaaca aggatgcggt caatttccgc ctgctgcgca cccccacggc tgacccgtcg
    15541 ccggccaatt ggaccgaggt catcccggcc cgggattcgg tgaaactgga cggcgtcgac
    15601 tgcttcaagg actatctcgt cgtctacgag cacgaccgcg gtctccccat ggtccagatc
    15661 cggaccgtga gcgacggcgc cgtgcactac atcgccttcg accagccgat ctactcggtc
    15721 tatccgaacc gcaacccgtc gtacgatgcg tcgaagctcc gcctcaattt ccaatcgctc
    15781 atcacgccga gcacgatctt tgactacgac atggattcgc gcaagcgcac cacgctgaaa
    15841 caggaggaag tgctcggcgg ctacaacccc gaggactacg cccaggagcg ggtgtgggcg
    15901 acggcaccgg acggcacacg gatccccatt tccatgtgct accggaaagg gatggtgaga
    15961 gacggttccc acccgctcta cctgtacggt tacggcgctt acggcatgac gatggagccc
    16021 tggttttcgg ccaaccggct gagcctgatc gaccgcggtt tcatctacgc catcgcccac
    16081 atccggggcg ggggatacct cgggcggccg tggtacgagg acggcaagat gctgcgcaag
    16141 cgcaacacct tcaccgattt catcgcctgc ggcgactacc tggccgaaca gggctacacc
    16201 gcgcccgacc gaatggtgat ctcgggggcc agcgccggcg gcctgctcat cggcgcggtc
    16261 gtcaacatgc gccccgacct ggcggccgtg gccatcgccg atgtcccgtt tgtcgacgtc
    16321 ataaatacca tgctcgatga gtcgatcccc ctgaccgtga tcgagtacga ggagtggggc
    16381 aatccccatg acccggccta ctacgattac atgatgtcct actcgccgta cgacaacgtc
    16441 accgcgaagg attatcccgc gatgctcgtc acggcagggc tgaacgaccc gcgcgtgcag
    16501 tactgggagc cggccaagtg gacggcgaaa ctccgcgcca ccaagaccga tgaccacgtt
    16561 ctgctgctca agactaacat gggggcgggg cacggtgggg cctccggccg ctatgattac
    16621 ctgaaagagg ttgccctgga gtacgccttc attctcgacc agctcggcct gaccgggccg
    16681 tgagcggagg aagcggcctg ggcgcaaacc ggagggccgg ggacccgacc ggggcgcccc
    16741 ggcgcagtcg tcaggcaaac aagaaactcg cccgggcggg gacaccgcgg gcgagtttct
    16801 ttaggggagg tctcatgaag cgacggcagt ataataggcc ttatggatca agtcaatctt
    16861 tttttgcacc aaaacgcgac tttttttatc ccctcgtcgc gccggtcccg gacagcaaac
    16921 caaggcacgt atcctattgc gtcacaaact attgcgttgt cgtgtggccg tcgaaagaaa
    16981 ggaaaacggc gctccccagg cgggacagcg ccgttaaggt ccatcggaga atgacctcag
    17041 actgatccct gcggcggccg ctcgagccgc ccgaggtgcc gctgggcgac ggcgaagtag
    17101 atgaggaatc cgatcccggc caggacaagc atcagcccga acacgccccc atcgtcaaac
    17161 atgtccggat tcatgtagcc gaccagcagc gcaatcccga gcccgaccag caccatgccc
    17221 cacttcagcg acgacagccg ggccagttcc gcctcccgga gaaggaaacg ctgcgccttg
    17281 tcatccaccg gtcccttctc gaggatctgg cgccggagct tgtagtccag caccagtttg
    17341 acgatgacga cgccggtccc gaaaatcacc agcgggatca agacatcggt ccacataaat
    17401 actctccttg catcgcgttt ccgtcctgtc tgaccgattc gaccgcccgg cgcggcgttt
    17461 tgttgcaccg gagaatgcat tttctttgat gcaacaccgc ggccgcctat actgtcttta
    17521 ggtatgtatg gagcaggctg cgccggacaa ccggtcctta attgaggaaa tcctccgcgg
    17581 cagccaggcg gcgttccgga cgttcgtgag ccggcaccag cggctggtca gccacattgt
    17641 tttccggatg gtggcgaatg ccgccgaccg cgaggatgtc tgccaagacg tgttcctcaa
    17701 ggttttccac aacctcggca gcttccgcca tgacgccagg gtgtcgacgt gggtggcccg
    17761 gatcgcctac aacgcgtgca tcgaccacct gcagaggcgg aagatcccgc tgctggaaga
    17821 tctggccggc gaggacggat cgtcgccgct ggagacgatc gccgatccgg cggaaggggc
    17881 ggatgcggcg gctgagacgc gcgacctgtt tgcccgggtg caggcggaga tggcgtatct
    17941 gccgccgcag tatcgggcga tcctcacgct gtaccatctc gacgagatga cgtacgagga
    18001 gatcgtggag atcaccggcc tgcccatggg cacggtgaag aactacctgt tccgggcgcg
    18061 ccggcagttg cgcgaccggc tgttgacgaa ataccgaacg gaggacatat ggttgacagg
    18121 cacatgaccg acgacgagat gcaggcgtat ctggagaccg ggacgtcggc gctcgaccag
    18181 gtgttcgagt cccaccttgc cgagtgccgc gactgccggg agcgggtgga gagctatcgg
    18241 gccctctttg cggcgctcgg ccgggatccc ggctgggtgt tgtcgccggg gttcgtcggg
    18301 gcggtcgagg cgcggctggc cgcggaccgg gctgccggcg ccaagcggcg ctggtaccac
    18361 gccctctggt ggctgggagg gatggcggcg gcgttcgggg ccacggcgta cttcatgggg
    18421 ctcggcactt tcacaggcta tctgcgcggg gtgttcgagc cgtccctgca gcatgggaca
    18481 cgcgcgttca acaacgcccg ccaggcggtc ggaacgctcg agggggtgcc gcagtcggtg
    18541 gctggggacg gcggcaatac gctcatagtg gtagggatct gcctgctcat tctcgcagcc
    18601 gtggccgggg ccgatcacct gatctttcgc agccgccagg ccaagttctg tctctgacga
    18661 tcccgcccgc caagaggccg agtccgaacc gggccgacga ggccggcgcg accggctatt
    18721 tcaggtagta cgcgttggac agccggagaa tgtcgttgat gagggaggcg ttgagcgagg
    18781 ctccgggcag ttcgttgagc gatgtgtcgg taatatcgaa agtgcggtcg aagttgatgc
    18841 tggtgatgcg atcaggttcg agcacggcga agttgtcctc gctgccgacc agcagccagc
    18901 cgcgtccggc ggcgtcggcg aggttgcggc cgatgctgta gtcgccgaca gggttttggc
    18961 agcggaacag acgctgcatg agagtcggta caatgtcgaa atggctggtc cagtggcgag
    19021 cgacgggtgg ttgggctgcg ggcgccgaca gcgaatcggt cggccgcagg gaatcgggcc
    19081 agcggatgat cagcgggacg cccagctggg cgcgcgagta gttgccgttg tgcccccagt
    19141 aacccttgcc gttttcgttg aattcctggc cgtggtcgcc ggtgataatc acgaccgtgt
    19201 tttcaagcaa accgtgcgcc tcgaggtcgg ccagaacgag gccgacgagc gagtcttcga
    19261 agtaggccac gttgcagtag aggttccaga agggggccgg gtcggtgtcc tcgcccaacg
    19321 ctgagtactt ggcgtgggtc caggcgggct gaaaggggcc ggtgaaatcg tccgggtggc
    19381 tgatcgagtg gagggcatcg tagaagagaa agccgaagaa ggggggggcg ccggtggagg
    19441 cggcatagtc ttcagtgaac gcgagccagt ctctggtaat ctgaaggtcg cggtcgtgcg
    19501 cgcgctcgcc ggggacgcgg aggggaatgc ccgggacgcc ggcgaacacc gtctgatcga
    19561 aaggcgggct gacgagactg gccgaggtga agaccccgaa ccgatagccg caggcaagca
    19621 ggtggttaat gaataccggg cggatcttgg ccgcgagcac ctcataccag taggtgccgg
    19681 ggaggctgta gaacaacgag aagatgcccg tgcgggtccc attggagccg ctgtagtggt
    19741 tgcggtactg ctccgccgag gcggcgaaat gccagaggcg cggcgtgacg agcgagtcca
    19801 gcgcccggta gttccaggag tcgagcagga tgagcagaat gttggcgccg gagccacagt
    19861 cggcgcggag gggggccagc gggtagcgga ggtacttgcg ggactcatcg gcgccggcgg
    19921 ccagagcgag ctggttctgt tccggcgacg cccagccgtg tttgatgaaa aaatcgttgg
    19981 cggtggcggg gaagtagagc gggtagaagc gcgaggcccg ggtgatcgag cgcgagccgt
    20041 tggcggccgc ccaggcatgg acggcgtggc cggccagcat catcaggagc accgccggga
    20101 ccgcccagcg ggcgacgcgc aggggacgcc accactgtcc gcgccacacc cagcggaaga
    20161 ggaacagttc gaaaaggatt ccgagaacga gcacggtcgc gacgaacgcg tactccgagg
    20221 ggtgaaattc atagatctcc gcgccggccc cgccgaaaag cagctcgagg gtgaagcggt
    20281 tgatgtggaa gcggtagagg ttgaacacgt gagtgtccag agccagcagg ccgagggtaa
    20341 gcgcggcgat caacgccccc cacggcagca gcacgccgcg gcgcggcacg aggagcgcca
    20401 ccggggcata gagcgccagc caggggatga aaaccagggc cacaaagtgg ctgagggtca
    20461 cgatggcgat gtacaggtag gccccgaggc ccgcgatcgc ggtaaagtgg accatgtagc
    20521 gggtcgcgat gaggatggcg aacccggcgt tgatcaggaa gaaccaactg ccggcgcgga
    20581 gagcatcgcg gcggggtgtc ggagacacgg tctgcatagg gtgagataat cgtagggcga
    20641 aaggaggggg aagtcaaggc ggttgtgacg cgcgcgggcc ggggcgtgcg gacggccggc
    20701 gagggccgac cgcacgccgc cgccccgttc acttgttctg cgtgtcctcc gcggttcgtt
    20761 tctcggcgct gaagacgccg ttgggctgct tgaagcgaag accggtaatc cggccatcct
    20821 tggcgcggtc gaacgcgaca ctgtacccgg acaggtctct gaaggtgaat tcgtcattcc
    20881 ggtagggcag gagggtgtag gtcggctggc cggggacagt gacagtcagg cggtcggcct
    20941 ggatggcgac ggtcacagca agttgctcca agaggtagtc gccggtgaac tgggccaggt
    21001 aggcgggttc cgagaggcgg ctgtccggcc ggcggacgaa ttcgataggc gggagagtcg
    21061 gttcgagaac gacgctgagg cggtcgatgt cgccgaaggt gttggtcgag aaggagagga
    21121 aggaccgcgc tttcttgtcg gccagcggct catcgatcat gcggaaaaca tcgtaatgcc
    21181 agtgctccag atccgactcg agggagtgca ggacagcgcg gaggtgctgg tccttccggg
    21241 gaacgccggc gaggctcacc gtaacgacgc cgtagccggg gtgttcgtac tcccccgcgt
    21301 aggcgctgag gtcgtgcgag ggtttggtgt tcgggacgcg gtcggcggcc gcctgggcct
    21361 cggttccccg gctgctgtcg gcggcggcga gctgggtcag ggcgcgccga tggtagtcga
    21421 ccggctcaag gccgaggagg aggtcatcga cgtagttcgc gacgatcgag gggaggggcg
    21481 tgccgttctt gttggtgagg atgaccaggc cgagccggtc gtccgggagg aacgacgcga
    21541 gggcggagaa gccgtcgatg ttgccgccgt ggtggacgcg gtagtggccg cggtaggact
    21601 cgatgaacca gccgagaccg tagctcgtga gaatggtctc atggtagcgg ccaccggcgc
    21661 gatcgaggga gacgcagggg gagtgcatct caatcatctg gccttcgctc accagtcgca
    21721 ccgtgtcccg gaggccgttg gccaggtgga ggcggatcca gttgaccatc tgatcgacgg
    21781 aggagttgat ggcgccggcc gggccgatcg cctcgatcgg acggtacggc atgagcgcca
    21841 gggtgtcgtt cttctcctcg taaccgcggg caaactcggg cgccttctcc aactcggcga
    21901 tcgagaagtt tgagttggtc atgccgaggg ggaggaaaat gcgggcgcgg acaaactcct
    21961 cccaactggt accggcgacc ctgccgacca gatacccggc cgtcatgtac atcaggttct
    22021 ggtactggaa ttcgctgcga aagtccttgc tgaagtccag gtagcgcaga cggcggtaca
    22081 tctcctcgcg cgtgcagtcg gagctgtacc acatgaggtc gtggcgcggc aggccgatgc
    22141 ggtgggtgca gaggtcgcgc acggtcgcct ggtcggtgat ctcggggtcg gagaggcgga
    22201 aatcggggag gtagtcgcgc acccggccgt cccactccac cagcccggag tcgacgagca
    22261 tggcgagggc ggtagtcgtg aaagctttcg tggaggatcc gatggggaac agggtgcggg
    22321 gggtgaccgg gaggttgttc tcgcggtcgc gcagaccaaa cccctccatc atcagcacac
    22381 tgtcgtcctt gacgatcgca atggccaggc cggggacttt ccagggggcg agcgcggtgt
    22441 cgatgaacga gcggatccgg gcgaggttgt ccgccagagc cgcggcccgc gcctgccggg
    22501 cagccacatc gaggcgggtc atggagaagg ggaacgcctg gccgccctgg gtgaagttac
    22561 cggcgacggc gccggaatca gcggcgaagg cgccgcggaa gaccggggcg ccgggaattc
    22621 cggcgagggc gaagatgagg gaatcgtcgg tgacgatgat gccctcgacc ggcaggtcgg
    22681 cggcgttctg ggcggggatg tcgaccacgg cggcccaggc gccggcggaa tcccggctga
    22741 attccacggt gaaagcgagg gcagaaccgg ggatgtcgat cgagccctcc cacccgccgc
    22801 tcggatcagc ggcgcgaaca cccgaggcaa ggcacacacc ggcgagcgcc gcgagagcgc
    22861 tcgcgcgaag gcggttgcga acagcagaca tgaggtcctc cacaacgaga atatgggtca
    22921 cagcctgtcc ttaaggacag acggaggaaa tgtttgcgat gttgcgcggg gaacgcaaga
    22981 gcattcggcg gggaagaggc cgctttgcac gccgacgccg tccaccgcaa taaaaagccg
    23041 gcgcgcgccg gcagccggcg gggagggaaa gaccggccac cggcattccg cagccggcga
    23101 aatcagcgct atttatacaa catcaggtgt tgtagtacaa ctcaaattcg tgcggtgtgg
    23161 gacgaatttg tatctgggcg acttcttttc gcttcagggc aatccagttt tccaccaggt
    23221 cgcgggtaaa gacccccccc tggagcagaa agtcgcagtc gttctccagg gcatccagcg
    23281 cccccgtcag ggtggtgggc agctggtggt gggccttgag ttcctcgggc gtgaggtggt
    23341 cggcgttgcg gtcgaggggg gcgcccgggt cgagtttgcg cttgatgcca tccagcccgg
    23401 ccatgagcat ggccgcatag gcaaggtaga agttcatggt gccgtccggg gggcggaact
    23461 caaaacgcat cttccgcgga tcacgctggt aaccggggat gcggatacag gcagtcctgt
    23521 ttcccatcga gtaagaaccg atgatagggg cttcgaaacc gggcaccagg cgcttgaacg
    23581 agttcgtcga cgggtttgta aaggccagca gggcatcgac atgcttcagc agaccaccga
    23641 caaagtgcag gccggtctcg gagagcatcg ccgggccgtt cttgtcgtag aacaaggaac
    23701 cgttggcatc ggcaaggtac tggtggacgt ggaggccgga accgggttcg ttgaagagcg
    23761 gcttgggcat gaaggtggcg gatttgccgc ggcggaaaca atggttcttg ataatgtact
    23821 tcacgagcat cgacaggtcg gccatgcggc ggaggggaga aaactccacc tcgatctcgt
    23881 gctgtccgcc ggaaccgacc tcgtggtggt ggtacttaag atggacgccg acatccgcaa
    23941 gcaggaccgc gagttccgag cgcaagttaa aggtgcggtc catgggcgga gcgatatgat
    24001 accccttctt gtggggaatc ttgaagccga ggctctcctc gtcggccgcc gcgctccaat
    24061 cggcttcgac cgagtcgagg tggtagaagg cgctctcggc cccctggaag aagttgacct
    24121 tgtcgaatac gtagaactcg aattccgggc ccagaatgca ctggacgccg gggaaggctg
    24181 cctggaggaa acgctcggcg tcggctgcaa cccggcgggg gttccgggag tacgggagga
    24241 tccggtccgc cgattccatc acgtccccga tcatggagag cgtcggacgg tcgaagaagg
    24301 ggtccatgaa ggcggtcgcg gtgtcgggga taaggatcat gtcgccgcgc tcgatcgagg
    24361 agaaaccggg gagggaggag ccgtcaacgc cgacgccctg ttcgaagagg ctgggtttca
    24421 gcgcggcaat gggaagggtc atgtgatgcc acacaccgag aagatcgcag aatttcaggt
    24481 caaggaactc cgcccggtgt gattcggcaa gtttttgcag ttcggccaga ttcatgtatc
    24541 gtcctttctg ccttacgagc ttgtgataca attcacatta tatgacatcg cgccggtatc
    24601 tgacaaggag attctttcgc cgcgcggcat accgcgagag gcggcgggca aagaaaaccg
    24661 gcgggtcgga acccgccggc tgggaatgtc catggggcga gggtctcaca tgatgatggc
    24721 gactttgccc atctgggtgt tgccctcttc gtcctccacc gtccagtagt acagaccgga
    24781 cacgaccatc tgcgtgttgc gcgtgatcag atcccaggaa tcatgggacg cattgggatc
    24841 gctcggatcc atgtcgtgct tgatctcgcg caccaggtcg ccgtcgagcg tgtaaatccg
    24901 gatcgtgcac ttggccggca gattggcaaa gtgcaggtcg cgcacccggc tgtcgggccg
    24961 gttgatgtcg tcggcgccgc gcccctcgaa gcccagagcg cggtacccgc cgtccgatcg
    25021 atacgggttc gggtacacga tgacatcgag attctcctgc tccacggcct gcgccgtagc
    25081 cagcgggtaa gccatcttcg aaccgaccgt cttggaggtc tccagcgacg acaggccgga
    25141 ggagggcgaa ccgtagtcga acgccgtcac gttcacgtag tacgggacgg tggggaggag
    25201 cgagtcgatg atgtactcgt actcgaaata cttgaagaag ccgtcgggag tcagttcgct
    25261 cggatccgcc tcggaaggaa tcaggctgga ggggtaaggt tgatccgggt agattttgtg
    25321 gatgcccttc aggttggtca gatccgacac gttgtagtcc tgcgcggcga agtagtactg
    25381 ggggaagcgc ggatccgggt tcacggccgg cgtgtaggcg cgcgagaagg tccacgcacc
    25441 cgggtcgaga gtgggatcct ggaagagagc gcgcagggag tcgagcgtat agggcgggtc
    25501 gttgagctgc cagcccttgt ccagatcgta gtcccacttg ttgaagtcgt gccggtcgta
    25561 ggacgccagc acgctgaagc tggtctcccg gttgtcgcgg ccgaggtaga tccggtagcc
    25621 ttcgaagtca ttcaggccgg agaagcggtc aaccccggtt tcggaggcct tgccgttgaa
    25681 gcggacccgg atttggccga ccttgggttc aagccagatg cgcggggcat ccggcggccg
    25741 ggcgccgcgg aagtcgggga tgccgtcgcc catgtaagcg actacctccc actgcgcacc
    25801 cggcgcattg gcggccgtcg tgtcgcagtc ctccaggcag atgcgagacc cgtcgggcag
    25861 ggtcgccatg cagcgaaact ttcccgaata accgtcgctg tcgctgtcga cgccggggtt
    25921 gtcataaatc caggaggccc aggtggcgtt ttcggccagc gaatcgaaat agaggttgga
    25981 gtagtagaga accggatcgg ccaggagatt gtcgccgtta tcgggatccc gatggagatc
    26041 ggccccggcc acataggcga atgaaagcgg cagcttctgt cccttgcgaa tgttgaacgg
    26101 tccgaaggag agcaggtagc gggtatcgaa tccgtcggcg atgtccggcg cgagcgccga
    26161 gggcggctta atccaactgg ggtcgccctc gcggatcgtg ttggtaaaga tctggtcgta
    26221 gtcgaactcg cggttccgga gcgagtagta gcggttgcgg tcgccgaccg gggtgccggt
    26281 gccgccggtc cggaagtcac ggtagggttc tttccagcgt ccgaccgagt ccttctcgcg
    26341 cgggccgaaa tcgagcgcgg cattgccgtt gctcacccac cagttgaaag agacatcgag
    26401 cgtgtcggcg ggggtgcgga cgatgcgggt gccggtgacg tgcggcgtgg actgaatccc
    26461 ctcgccggcg ttgaagtcgc cgtcgttgtc cgcgatccag gcgatgtaca cggtgtcgtt
    26521 gtactcgcag cgcatgcgcc cgtattcccg cggataggtc acgaggaagc cgcaaatgtc
    26581 gtcggcaaac ccttcgttgt cctggcagtt atcgcacacg tcggcatcca catagacgcc
    26641 catatacacg ttgcggaggt cgatgttgcc gatatttttg atttcgtagt cgaagaggat
    26701 gatgtcgtcg gcatagtcat aggaccacgc ataggaacgc tgggtgacct gaatgttcag
    26761 cggccggtgg gcggtgccat cctcctcggc atcggccagg atcgtggagg tgtcggtata
    26821 gagcgcaatg aaatcctgtt ccgagacggc attgacgtac tcctccgact cggggtcgat
    26881 aatcgagcgg cggacagtat tgccgaaagg ttcgtcattg gggaaaaact gccggacatc
    26941 gctccagccc tccgaaccgg tcgacacaag ggtgtcgcgg ccgaggacgg cgccgatcca
    27001 gaaggcggcc gagaacatat acatggcgcc cgtgcccttg gggtactggc agccgccggg
    27061 cggaggggtt tggcccgtga agcagtccgg agcgtcgctg ggcacggaga attccgaacc
    27121 gaaggttccg ttgttgttga cggccaggac gaggcggccg acgttgtgca gggtcatgca
    27181 gtaggccggg ctgcggctgg ccatgggccg gttgtacccg cggccggaaa ggccgccgtc
    27241 gtcacgggcc tccgcggaac cggatatcag caacaggcct gcggcaaggg cggcagtgat
    27301 agtgagcact cttcgcaaca tataccttcc catcggatta aaccgtttta cctgacagac
    27361 gcacaggacc gctcaggcgt ttaatcgcat tatgcaaagt cacacacggg gtaaactaat
    27421 gtcggtacat cggataaagc aaggaatttc ggcgaaaaaa accgccctct ccggaggtcc
    27481 gaccgcccgg ctgcccatcg gtctgaaatg tttgccaaat ggacgtttag acgagttcgg
    27541 gggatcgcag attctgccgg gccgggacgt gcaaactccg cagggcgccg tcgctcccaa
    27601 cgcctctggc cgccaatgtc cgggtccgcc aaaaaccccc gtgcaaacac cgagtcgttg
    27661 cggccgggcg ttgaaacccc gatattgcgg atcgttaacg aaccgccggc gcgtggggga
    27721 attgcggagc cgatgagtga ctgagtttcc ggtcagcggc gtcgagacct ggtggtggtt
    27781 gccatcgctg gtggctttct gcgtgtcaac gctgacttcg accggcgggc tgtcgggggc
    27841 attcctcatg ctgccctttc aggtgagcgt gctaggcttt accgggcccg gagtcacgcc
    27901 cacgaacctc ctgtacaacg tggtcggcat accgagcggc gtctaccggt acgggcggga
    27961 gaaacggatg ctctggccgc tggcttgggc gatcgtgatc gggaccatgc ccggcatggc
    28021 gatcgggacg gtcatccgcg tgaccctgct gccggacccg tcggcgttca aagtgtttgc
    28081 cggattggtg ctcgcctttg tcggcgtacg gttgctccgc gatgtggtgc gtggggaagg
    28141 cgcaaggcca tcaccgcggg gcaacggggc ggggcggttc gaggtctcgg ggggacggct
    28201 gacgaagctc actgtggagt acgatttcgg gcgccaacga tacacggcct caactccgaa
    28261 aatcctcctg ctgagtttcc tggtcggcgt ggccggcggg gcttacggga tcggcggcgg
    28321 cgcggtcatt gcgccgtttc tggtctcggc cttcggtctg cccgtctaca ccgtggccgg
    28381 ggccgcgctc ttcggcactt ttgtcagctc ggcggccggt gtggccatgt tctggctgat
    28441 cgacctctcc gggttgaccg gcagcacggc ggcccagccg gactggctgc tgggtctggc
    28501 cctggggatc ggcggggcct tcgggacgta tacgggagcg cggctgcaga agcgggtgcc
    28561 ggcgcggggc ataaaggcgc tcctgacagc gttcctgctg tttgtcgccg ggcggtacat
    28621 agtggaagct ttctgacaca acagggtcac gatatggatg acacgattgc gaaattcgcc
    28681 tccccggcgc cgattgccgt ggtgggcgca accgacgaga cgcggaaatt cgggggctac
    28741 ctgtaccgcg aattgaagcg gcaggggtac cgggtgtacc cggtcaatgc gcggagagcg
    28801 gaggtcgacg gggaccgggc gtaccggtcg ctgcgggata tccccgacga cgtcgaggga
    28861 gcggtggtcg caatccgtcc ggaccgggcg ggggaggtgg tggcggacgc tcttgcccgc
    28921 ggcattcggc gcatctggtt tcagcaagga gcggatttca ccgaggcggc ccgcacggcc
    28981 cgagaggcag gcatggagac ggttgtgggg aagtgcattc tgatgtatgc cggaaaggcc
    29041 cgcggcgtgc accggtttca cgcggtgctc gccaggctct tcgggcggta ctgaaaatca
    29101 gacggaggac gcgcgtggcg acggagattc gggaaattga ggaccgcgac ctggacgcct
    29161 tcattcgcct ccagatcgac gcctatccgg ggctgggtat gatctacgag cgggaccggg
    29221 agaaactccg gcaacggatc gcggaacggc gccaggatgt gcgcgtgacc ctctatggaa
    29281 gctaccgggg gaaccgcctg gtcgggtgca tgcgggtcca tgactaccgg atgaatttcc
    29341 gcggtgcgct ggtggcggcg ggcgggctgg gcggagtggc ggtggatctg acggtcaagc
    29401 gcgagggagt ggccggcggc atggtccggt tttacctcga caagtatgcg gccgccggcg
    29461 cgccgatggc ggtgctgtgg ccgttccggc cggacttcta ccgcaagatg ggtttcgggt
    29521 acggcggcaa gaaataccgc tacacgattc tgcccgagac gctgccgcac gcggccggca
    29581 agggccacct ggcccacggg gaggagaggg atttcgccgc catgacgcgc tgccataacc
    29641 ggctgctggc gctacggacc ggcatgctgg acgagaccga gctgtcgctt cgccagacgg
    29701 tgggggcggc cggcggaggg cgcgcagtcg tgtaccgcga cgggcgcgaa gtgcgcggct
    29761 tcatggtcta tcgctttcgc cccggggcgc cgggcaattt cgtcagcaac gatctctgcg
    29821 tggaggagtg cctgtacgaa tctcccgaag tgctggcgga gttcctggcc tttctccaca
    29881 gtcagcgcga tcaggtgcgg cgggtgattt tggacttgcc cgatccgaat ttccactacg
    29941 cgccggtcga tccgcgcaac ggttcggagg gcctttacgc ctcggtgtac cacgaggtgg
    30001 ccgcctgcgc cgtggggccg atgtaccgcg tcctcgacgt gcagtcgctc ctggcggcgg
    30061 cgtcggaggc gaaattcggg ccgggcgagg caaccgtggc gtttacgctg gccgaccggt
    30121 ggtggggaaa aaccgccggg acgaccgcca tccacttctg cgagggcaga gccgcagcgg
    30181 cgccgaaaag gaagtgcgac gtggcggttg aaatggaggt cgccgatttc tcctctgtga
    30241 tattgggggc ggtgcggctg tcgcaactgg tggattacgg cctggcacga gtgtcgaagc
    30301 gcaaatctgc agaggttgtc gacagcatcc tggccgccgc ccagccgcct tactgcttct
    30361 caagattctg acgcgcgttc cgcggtccgc ccgacgtcac gatccctcaa gccggggcgg
    30421 cggcgaccgc cgccgccgct caacacggcg ccggggccgg accgccgcga aagaggtacg
    30481 cgaccaggaa ggtcaagtcg gcgatcccga tctcgccgtc ggcgttgaca tccgcctcgg
    30541 ccatcacggg aggcgcgggg ccgccccgga aaaggtaggc gacaaagaac gtgatgtcgg
    30601 ccacgttgac caggtcgttc aggtaaccgt cgatattccc ccggatgccg gtgcagatgt
    30661 cgaggacttc gcaggcgtcg ccggtgccgt cggcgtcgct gtcctcctgg agcgggttgt
    30721 agtggccggg gcagttgtcg gcggcgccgc agatgccgtc cccgtcagca tcgttggaag
    30781 gatcggtcgg gcagggatcg cacagatcgc cgaggctgtc ggagtcggcg tcggcctgtg
    30841 aggcattgga cacggccggg caattatcgc aggcgtccgc ataaccgtcg gcatcggcgt
    30901 cctcctggcc gggattggga tcattcgggc agttgtcgca ggcgtcgccg aggccgtcgc
    30961 cgtcggtgtc gagctggccg gcgttggcga ccgcggggca gttgtcatac tgggcgcaga
    31021 tcaggtcgcc gtcggaatcg tcgcagcagg ccaaagagcg ggcctctccg cctacccaga
    31081 agaaggggaa tccgggatcg taaacccaga agatgttcca ggcgtaccag gcggtgtcgt
    31141 agaactggaa ggcgtcgcag agggccgggg aggaatcggt gtcccacatg atggagggga
    31201 gacgcaacga ggtggtgtcg gtgtacctga tgccgatgaa gaagggtgcg tcgaagcagc
    31261 agggagaggg gagagggacg gtgccgacct gcgggaacgc gaaagaggcg gagtcgcagg
    31321 cagccgaaac cgagcacagc agagcgccgg gaccgtcgca cggatccccg gacggggcca
    31381 gggcgtagac gaccacctcg agctcaaccg gccacttgta gagacgggaa tcaatgtagg
    31441 acggcgggtc gaggagcgtg aaggacaatt cggtgatgcc gaagggatag gtggaatcgg
    31501 cggcacagga agtcgggtcg aagtagacga tcgtctgctg gccggcagtg aatgacgaac
    31561 tgtagccgag aacggaatcg tcgggattat ggcggaagag caggcaagtg tccgtgctgc
    31621 ggcccaatcg gtcgaactgc acctggcgat ccgggcggaa tgtctcccag ccggtttccg
    31681 gggcgccgaa ggcggcgctc gcagtgcaca ggtacaggca ggcgaaggca agacatttgg
    31741 acattggcag cctccagtgt tacgtggccc cgccgccggc aggagtttcg gcggcgacgt
    31801 gcggccagag caacggtgta cgcattcagt ataccttgtg ccgcggcagg gcacaagcga
    31861 aaatgcgggc aagcgaaccc ccgcctcagc agtcggctga ggcgggggtg agtagaaaag
    31921 ctgctatcaa tggcagatgg gaggcggacc gctgcggaag aggaaggcga ccaggtagg
//
LOCUS       DAOPOE010000025        31822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1851_length_31822_cov_6.272704, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000025 DAOPOE010000000
VERSION     DAOPOE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31822
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_31822_cov_6.272704"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PKM94_05160"
     CDS             complement(<1..1414)
                     /locus_tag="PKM94_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOD66064.1"
                     /translation="MGSLVLTAAAVVVAAAAGAVDVDSLLVQSVGGPEAYRTLAAMTS
                     YRTEGKADINGLEGTYTQVYAAPDRFYMEIRIGGMTLVSAFDGRTAWQRDHNGRVSEV
                     EGMEKRQLIEQVYLASYSFIISGRLPGRSEFLGDTTIADTSWLRVALYPRPDDTVVML
                     LDGQSALPAYVVQRLDNLTGVTRLSDYRTVAGVALAHSARTEFPEAALYTEMTTESVA
                     FNVEVDPALFDLPGAEPVDYRFAAGADSVVIPFTYYRGHVWLPAAVNGATVAWFILDS
                     GASANLLNDALTAPLGLTSVGSVPAKGVGGFEEVPLVRTDSITIGSLTLVDQIAAAMD
                     WDRLGIEPPPGAQAGGILGHDFLSRFPVLIDYSDSTLTAYNPAAFTAPAGGTAVDFHL
                     TMLVPTISASVGGVEGDFIIDLGNSVGLVLHDRFFQDHRLDTVLTNLASVDRPIGGVG
                     GTARTRSAVVPAFRIGGMVLS"
     gene            complement(1654..2022)
                     /locus_tag="PKM94_05165"
     CDS             complement(1654..2022)
                     /locus_tag="PKM94_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66065.1"
                     /translation="MSKRVIIAANNMIFTSKIMTALDSLKAEGIKAIRSEEIFSKAQD
                     LQPDLIIIDLNLNGIEAPALINKLRSYGATKQIPIVCYSPNLLKDQMKAAKVAGATEV
                     YPNSQMTSRMNQILGKYIQN"
     gene            2283..3710
                     /gene="pyk"
                     /locus_tag="PKM94_05170"
     CDS             2283..3710
                     /gene="pyk"
                     /locus_tag="PKM94_05170"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOD66066.1"
                     /translation="MKHTKILATFGPAIDDPDRLRELVSAGVNLFRVNCSHGESADFC
                     RAAATIRQATARACFPIGLLFDISGPKLRLDRFEGSLDIAEGTELTLVADRTDLAHRL
                     IAVNHPAVIASLRVDDRLFMDDGQFLFHVTARTEQAVTVRSHSSGKLLGGKGINLPGV
                     PLPIPTITAKDRQDIETAVRCGADYIALSFVRSADDVVQARELIAAAGGGPKVIAKLE
                     KREAIDRLDEIMSAADGVMIARGDLGVELPPEVLPRLQRHIIKVANKFHKPVIVATQM
                     LESMRFNPRPTRAEVNDVASAVLDFVDAVMLSAETATGKFPVEAARMMTQVITATEEA
                     LPAASAVRVERTVTDPVPQAIADAVSFVQEQGTARVVFAFTSSGFTAQLISNLFSPYP
                     IIALTLNRCVMSALSLHRSVYPVLVEQPASFDEIARLVSAIGRAYAGVRPGDRVVVTG
                     GAPFGQMKPTNFMMIVTILDERESG"
     gene            3717..4631
                     /locus_tag="PKM94_05175"
     CDS             3717..4631
                     /locus_tag="PKM94_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD66067.1"
                     /translation="MWHMCSRLCRHSGRAAVVVLLLAGLSFTSAEAKGDHEICITFDE
                     LPAAQGFSSTAPDQVLRPILKALKKYDVKAAGFVVGQNLESSFDLLGQWLNDGHCLGN
                     LTYSYSDLNEVGAVNVEAFIADIVAGQQAIEPMLEGFGQQRRYFRYPFLHYGTSLEMY
                     RAVAGYLDANDIAVAHASVIVDDYLYDLGLQKVGGRPTDKEYEQLMNEYVNHVLDQIE
                     AAEVLAKEILGRPCRQILQLRANQLNAMYLDVMLQAIKDMGYEFISLDRALQDEAYQK
                     PHAYFGGKGVGYLEMIDRSDPDLLPAGE"
     gene            4696..5379
                     /locus_tag="PKM94_05180"
     CDS             4696..5379
                     /locus_tag="PKM94_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66068.1"
                     /translation="MRRARSFLLVLLATLPAAPAGADPLDRPGEAALSAGITWAEGDV
                     SRYGLRAFSVLGRVTLRPKPMSIFALWSNLSGNLFNSEEEVVSVPTDGGYMPATRSTT
                     ETAFALHLGVQLNPPAARSPIRPRVALGAGLWMFNSDIAYRVDREDDPFLSDDETSFE
                     FGLRGNGGIDVFFAEKWGIALDFTFDRLFGVDRVVPTDDAGGGRPAAQTARYFSAALG
                     LVAPLDRLF"
     gene            5667..6317
                     /gene="pyrR"
                     /locus_tag="PKM94_05185"
     CDS             5667..6317
                     /gene="pyrR"
                     /locus_tag="PKM94_05185"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOD66069.1"
                     /translation="MEVSLPAQTDLILDEKKIARALQRIAHEIVERNAGAESLVIIGV
                     LTRGAFLARRIARHIEELEGVKVPVGLMDISLYRDDVHSKLDQPIVQQTQILFAVVDR
                     NVILIDDVLFTGRTVRAALDQIIDFGRPRSIQLAVLVDRGHRELPFRPDYVGVNIPTA
                     RDDRVVLEVKEKEGQDRVYVIRAGGLKSRPAAPGTAPAKKTARAAGKKPKGKGSAK"
     gene            6314..7252
                     /locus_tag="PKM94_05190"
     CDS             6314..7252
                     /locus_tag="PKM94_05190"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOD66070.1"
                     /translation="MSRLSSNHLLGLEGVPRADIELILDTADTFREVLERTIKKVPTL
                     RGLTVLNLFYEPSTRTRISFELAEKRLSADTIAFTKAGTSVSKGESLRDTVQNIEAMK
                     IDMVVVRHSSAGAPYFLTQCVDANIINAGDGSHEHPTQALLDMYTIRKKYGRLDGLRV
                     VLVGDVRHSRVIRSNIWGLKTMGASVALCGPSTLLPREIEQFGCDSYTDLDEALDGAD
                     VVNVMRIQLERQQAGLFPSQREYTNLWGINRERLKRLNKNYTIMHPGPINRGVEITSD
                     VADGDRSVILDQVTNGQAVRMAVLYLLRGKDEGEVE"
     gene            7252..8562
                     /locus_tag="PKM94_05195"
     CDS             7252..8562
                     /locus_tag="PKM94_05195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOD66071.1"
                     /translation="MPKKSYDLVIRGGRVIDPALGFGREASIFIKDGKIVKMEKTSEG
                     SRDLKGMPEEKVIDAAGMIVTPGLIDVHVHLREPGREDEETVETGCRAAAAGGFTSLC
                     CMPNTAPAIDNQETVKFVQDRAQSADARVYVVGAITKGRKGAELSEIGDLVKVGAVAV
                     SDDGAYLQNAEIMRRALQYSRMFNIPVLQHAEDQQLCEGAVMNESFESTKLGLKGAPP
                     EAEEIAVLRDIALARSTGGRVHFQHITTKRAIEAIRQAKKEGIRVTAEAAPHHLVLND
                     AEIAKAFNTNLRVNPPLRSESDRQALIEGLVDGTIDCIASDHAPHSEEEKDCEFDLAP
                     PGMIGLETSLALINTFLIEKGLMNWADVIRKMTVNPAHILKLPGGTLAVGSSADITII
                     DPEKKWTVRADKFRSKSKNSPYIGWKLTGQVARTILGGRTVYQR"
     gene            8747..9727
                     /locus_tag="PKM94_05200"
     CDS             8747..9727
                     /locus_tag="PKM94_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD66072.1"
                     /translation="MPTLSRSRMARIAARTLANMARRRPINVSFEITYNCNARCEHCD
                     LGDYVKEPRLGPEVFADWLTRLKPAVAQISGGEPLLRKDLVDIVRAMRERDPVPVFVI
                     TTNVQIMTEKKYLQLREAGMDEFSFSLDYPDDRHSAFRHLKDNFRKMSELVPTLAAHG
                     HKDIVLACVVQSDNFRDLPKIAELAREWGVGVNFSTYNALRTGKKYYLITSPQDLAEL
                     ETIVDRLAAMQREGYPIMTSEWTMRQMIDFFRTGGHPRCRAGERFMIVNPWGKLTPCG
                     MCRDHYDDPAQIRERFSRTNECGDCYTAIRANSEKTPYRLIADALRVVRR"
     gene            9782..10696
                     /locus_tag="PKM94_05205"
     CDS             9782..10696
                     /locus_tag="PKM94_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD66073.1"
                     /translation="MPTPAPKAPTVLGHLGLFYAAAIWGATFFVVKGALAAVDPLILV
                     GYRFLIAGGLLLAVVIILGRNLTAHLGRSAILAVILWTLYISQTVGLQYTTASNSGFI
                     TGLFIAFVPILLRLLFRRRPTIMEVIASAVSLVGLWILTGGLRQVNVGDMLTLISAMT
                     YALHLLYSDKYLKEGLDPWVMSCQQFLLVGALSIAAGWVTGRPFAVRTAGAMWVVIFL
                     ALLPTASAFVIQMLAQRIIAPVRVSLVFAFEPVFAGVFAWTLGGEPFILRTAGGGLLI
                     FLGLILSALPDPPAFRPKRAPTARRRAT"
     gene            complement(10689..13583)
                     /locus_tag="PKM94_05210"
     CDS             complement(10689..13583)
                     /locus_tag="PKM94_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD66074.1"
                     /translation="MTFRMGFLTLAAVMLGAAPVVADKLVEVTLRDFAEAQQLEAAGA
                     TAVAAAGNLYLAVISDSGLARLTAAGVEAAVVAQGVAADRLAIDRSAPGAYRGPHAVV
                     FARDRLRLVQVDSRAAAEKDPELVPITDFPVPIRYSPARPAPQPAAGAMDTIDSLVAL
                     VSEDSLRSYTERLQAFYRRVSDTDSSRAARDWLAAKFAAFGYDSVVLDYFDPYPSSST
                     PPGECCNVVACRPGAAYPEQQIVIGAHYDAVASSPGADDNASGTAAVLELARVLAGCA
                     APVTLTYIPFDAEESGLLGSRHYADSMAALNREIVLMVNFDMIGHETNDSVARVYFNH
                     DSVYADLWVLLAPTTGPLVGDIAGYGGRSDHVPFGSNGYDFLSLTEDDFSTHYHSPSD
                     STTYLNFDYVERIVRVSLAAVYAAATLPPPVPIAAIRQPGDGRSLLVEWRPLRRTALD
                     HYRLCWYRQGSPATLQCLDLPPSDSIHTVEGLTEGAAYIFYVQAFDSAGQTSLTWPDG
                     IGIPQSRPAPPEGLTGLPIHQGVALQWGRNNQELDLSHYAIYRDGALAGTTSDTVFQD
                     QDPGLGEEFHEYLVAAVDDDGIVSDTIGRTPFEARAATLTPGRILAINRDNVAVPGAE
                     VALTGEFLRAALAGAACDYRSDSAYADASSRTRLYDLVSYELVVVAGETFYDNVAFSP
                     AGGGLLDTLAYYLAIGGKLLVFGRFGPSSGNTVVTIPYAATAAAWDDAYSTVFHLAER
                     SVLPLVAGGQVSGDLVGAHPQQAGWPALRWDSLRTAQQEPSIQTPFGICAGSFVTLNS
                     SLPDVLYTYDSGTDDTLTEGKAVAWRYLGADYRYIWFDLPLSFFDEAAAIAAVHRAVN
                     DLTGTATALPPEETLPDGFALTPNYPNPFNPRTTIGYTLPRTVRVKLTVYNLLGQQVR
                     VLVDGEQAGGRHEAVWDGTTGKGRPAASGVYLYRLETPSRCAARKMLLLK"
     gene            complement(13620..16502)
                     /locus_tag="PKM94_05215"
     CDS             complement(13620..16502)
                     /locus_tag="PKM94_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD66075.1"
                     /translation="MSRVLAAAFCICLAASAAAEDLLEVTIGSHRDARALRESALTPV
                     ALTGNVYLALGERAEWKKLVEAGVRIEPVAFDVARPLLAFDRRPPGDARPGYPAVYDR
                     HAFRLVALPNRSALAGPHAADLAPLVRGPVAVRYVPPRPAPQPAAGAMGTIDSLVALI
                     SEDSLRSYTERLQAFHRRLTGTDSSAAARDWIAARFLAFGYDSVAIDPFVGEQLWDRV
                     PVPSYNVVACKVGALYPEQEIIIGGHFDAVPDCPGADDNATGTAGTMEIARVLAAGDF
                     PMTLKFIAFDSEESWMWGSYHYADSVAAEGAEIVLMINLDMIAHESNTAGANLYWGAD
                     QSYAALWADLAPTYGGIAASLMGGTASDHLPFAENGYDVIFVQERNFSTHYHEPSDST
                     THLNFEYMTRMVKVSLATAATVWTLPPPVRITAIREPGDGQSQLVQWRAIHSANIDHY
                     RVYWYPDGSPGNAHNLTLPAGDSSALVTGLTEGVTYVFGVRAYDVQGRTALAVEEVLG
                     RPDSRPAAPPGLAALPISHGVRVWWRHTDHELDFEHYAVYRDGTLAGTTADSVYEDRD
                     PALGTELHRYLVAAVDFDGNLSDTAAAVEARAATLTPGRILAINRDNVAVPGAEVALT
                     GEFLRAALAGAACDYRSDSAYADASSRTRLYDLVSYELVVVAGETFYDNVAFSPAGGG
                     LLDTLAYYLAIGGKLLVFGRFGPSSGNTVVTIPYAATAAAWDDAYSTVFHLAERSVLP
                     LVAGGQVSGDLVGAHPQQAGWPALRWDSLRTAQQEPSIQTPFGICAGSFVTLNSSLPD
                     VLYTYDSGTDDTLTEGKAVAWRYLGADYRYIWFDLPLSFFDEAAAIAAVHRAVNDLTG
                     TATALPPEETLPDGFALTPNYPNPFNPRTTIGYTLPRTVRVKLTVYNLLGQQVRVLVD
                     GEQAGGRHEAVWDGTTGKGRPAASGVYLYRLETPSRCAARKMLLLK"
     gene            16668..17009
                     /locus_tag="PKM94_05220"
     CDS             16668..17009
                     /locus_tag="PKM94_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOD66076.1"
                     /translation="MIQIFGTRKCPDTRKAERFFRERGVAIQVIDLREKGVSPGELRS
                     LARAVPLSELIDTEGKEYERLNLRYIRHNVEEMLLAHPLLFRTPIVRSGPRAAAGYHP
                     EIWQTFAEAEK"
     gene            complement(17035..19680)
                     /locus_tag="PKM94_05225"
     CDS             complement(17035..19680)
                     /locus_tag="PKM94_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HOD66077.1"
                     /translation="MKVLVVAAAVIASLAGRALADIAMRIERSELARPGDRVTLTLAA
                     VDSTGAFQMGGFEMTVRYDTTLTLIAVNRGQMLNLCGWEYFTSNLIGVNNIHITAIAD
                     ISNGAHHPSCFGMPTADLVDITFDIPWDYSLYGRFLPVKYIWYDCGDNAVSSRWGDTL
                     YMSDDVYGFDGISEWEITRDTAFPTPYGAPSWCSSVAGGRILDFYSGGVSLIQQDFQP
                     PVAHCPNDTTVAAGPSECAAVVEFTATVTDNRPGATISCTPPSGTYFYRGANYVQCIA
                     VDAWGNRDTCGFTVTVTDTSAPILAALRDTVIGNAPNECGTVWTYSAATYDNCPGVTW
                     SGSPPPGSYFSVGATDVIVTAVDYSGNRKVDTFWVTVEDRQWPVAHHPDSVMVAAAPG
                     QCSAPATFSVTATDNCPLATAVAYPPSGSVFPVGTTMVQVIATDLLNNCDTTWFPVTV
                     RDTEPPQLIYPESLVVGVDSGQCAAAVTYTLSAVDNCGTPAVTVSLPPGSLFPLGTTA
                     VTAAAADLSGNVDSAAFYITVVDDEPPRLTAPHDLMVPAEPGVCSAMVAYAVTAADNC
                     GSAALSVNPPSGSYFPVGVSTVEAVAVDAAGNVDTARFTVTVRDEEPPAVTAPADLDL
                     PNDSGACGAAAAYEAAASDNCGLPAVTLQPSAGSFFPVGATAVRAVAVDAAGNADTAF
                     FTVTVHDTEPPTVQCPEAVEVTSDSGLYGAVVHFAIAALDNCAGALAAAEPPSGSLFP
                     LGSTVVTVAAQDAAGNADTAQFTVTVVLDDPDADGLPSWADNCAEDHNPDQTDTDGDG
                     VGDACCCAVRGDVNDSGLPGAQVNVSDLTALIAYLLQGGATPPCPEQANANGSTESGG
                     RITVSDLTFLISYLFRGGPAPSPCP"
     gene            19886..20584
                     /locus_tag="PKM94_05230"
     CDS             19886..20584
                     /locus_tag="PKM94_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66078.1"
                     /translation="MMDSAAYIQTDEVCRYYRRGASEVRAVDRVSLAVERGGFLAIVG
                     ASGSGKSTLLNLLAGLDTPTSGHIAVAGAPLGAMSRRELSAYRANRVGVVFQTFNLIP
                     HLTALRNVELALCFNGTPRPERRRRAAAMLDRLGLADRADHRPADLSGGEQQRVALAR
                     ALVKGPEILLADEPTGNLDRENTRQLADLLTAHNRDGLTVILVTHDLPLAERLAGRVV
                     RMHYGRLVEGTEAG"
     gene            20581..22053
                     /locus_tag="PKM94_05235"
     CDS             20581..22053
                     /locus_tag="PKM94_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66079.1"
                     /translation="MMTVRDLVEISAGNLWRMKLRTCLTLAGVVIAIGAFVAMVSFGA
                     GAEKNVSEEYEKLGLFTTMQVYPPAADSLGDSVPPPPLTDSVVARLARLPGVRLAYPY
                     DAFSVSVRLDTLELSAEAQVLPPAAVATRLYSRLVAGRRPDANGSREAMVTREFLEDH
                     ALGPADSILGRSIAVSVSSSTLDSAVVHVVRQESRRVEQFIDAFDFDSLDNVGYLRRT
                     ATGEFNAAARSFLEGYLGRPMITTDTLTIVGVIDGPQGRRLRVKPLIIPEQSARRFAA
                     AKSGPDPAELLAAFQSGRVGNLFGGDTAAPDAYSRVTLDLEPGTGYAALSDSIRAMGY
                     ETFSYLEQFAEIQRFFLYFDLMLGVVGMIALVTASLGIVNTMVMSILERRREIGVLKS
                     LGADDRDVWLQFLAESGVIGSVGAVLGILLGWVVTRIASFVAVRVMENQGMTAVELFD
                     LPLWLIAVALLFGTAVSVAAGLYPAARAARVNPVEALRNE"
     gene            22080..23075
                     /locus_tag="PKM94_05240"
     CDS             22080..23075
                     /locus_tag="PKM94_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOD66080.1"
                     /translation="MDRRSREAFAAGRTALLSEAAREWDFEPRTAAELDGFENHIFEV
                     ARGGRPFVLRLCHSLHRTAALVCAELHWLDFLARRGLPVAAPVPGRGGALARVIPAAD
                     SFFVASLCAKAPGRRPLRSDYSDRFFENWGELVGRIHRVTREYLPLAPPVRRFHWHED
                     PTLDPAPYRPAVDDDVLARFARIVAQLRALPVDRTAYGLIHNDLHPGNFFIDGDCLTA
                     FDFDDCHYQFLVNDVAMALFYALPLPARPRTAEAFAETFLPPFLRGYRRHAALGPEDL
                     RRLPMFLKLRELDLYSQIVLHLGADVGGWPGAYLEGRRDRILADRPVVDLDFAEF"
     gene            complement(23104..24054)
                     /locus_tag="PKM94_05245"
     CDS             complement(23104..24054)
                     /locus_tag="PKM94_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD66081.1"
                     /translation="MPTKPSRPGPAGALAAQRRTGYLLALISAVGGGAATATGKWNLV
                     HLSPLAMNALIFSLATVMLSAWVLPGYGPARVFRQTRRAWFWILLFSLSSWFAVLLVW
                     AGVQKMDPSLASFLNRSEVLVAIALGILFLGERLSRIETLGVLLSLAGIALMRLTLRG
                     EYSVGFGLVLLGSIGFGLTEFISKHAVRHVDPLILTYIRNGLLALGYWAVLFAAGGDL
                     AGLAQVWPGVIALALFGPLVSRLFYLQALARLELTKVAVISQSQPVVVMLIALALLHQ
                     LPTAREALGGLLLLIGCVIMAAAHRRPWARFGQQADTRAG"
     gene            complement(24060..24884)
                     /locus_tag="PKM94_05250"
     CDS             complement(24060..24884)
                     /locus_tag="PKM94_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66082.1"
                     /translation="MAARHYAILSISAAALLPAACGSDQDRFPTITYEMKTIERRFGT
                     EGDTVNGFAYLNVTYPEVTAASERGVADSVSARVWQFLLRPMAGAGTFETVDDMAKEF
                     IRNFKDRPDPARTASYGWTWLRKARVETDTLGILGVSLVETRYQGPEDQHAERRYVNF
                     DARTGRLLALSDLLIPGGDTLLNRTAEQAFRRARRLDLVESLAAAGFAFDSTAGFRHN
                     GNFLITPKGLAFYVNRGEAGKEELGPTGYTVPYTDLAALARPDGPLTPFVAPPPGL"
     gene            25089..26420
                     /locus_tag="PKM94_05255"
     CDS             25089..26420
                     /locus_tag="PKM94_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOD66083.1"
                     /translation="MTDAPSEPPDEIVPRKPPPVLSDDLTRRIAEQVLSGERVSRWQR
                     LLPFLGPAFIASVAYVDPGNFATNIQGGAEFGYRLLWVIVFSNLMAMLLQTLSAKLGI
                     ATGYNLAEHCRREFPTWVAWGMWVLMEAVAMSTDLAEFLGAALGFYLLLGIPLIWGAV
                     LTGIATFLILGLERRGFRRLEAVITAMVAVIVFAYVVESIMDRPDWGLVACHAVVPSL
                     PGPEAVLLAAGILGATVMPHAIFLHSALTQGRIQVRGAAQLQRLFRFQIIDVTVAMGL
                     ASLVNMAILIMAAATFHRHGLSEIASIEEAHRTLEPLLGSAASGVFAVSLLASGLSSS
                     TVGTSAGQVIMQGFLRRHIPVWVRRLVTMAPALIVIALGLDPTRTLVISQVVLSFGLP
                     FAVVPLVLFTARRSIMGVLVNRKPTTVAAAIVASLIIGLNVFLLYQLLFRE"
     gene            26424..27329
                     /locus_tag="PKM94_05260"
     CDS             26424..27329
                     /locus_tag="PKM94_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD66084.1"
                     /translation="MFERVLVPLDGSAVAEQVLPAVGCFAERLGAAVTLVHLIERRAP
                     AEIHGERHLRTAEEAEQYLGAIARRIAAGRLAVRTHVHTVEVASVAQSIVEHAEEFAS
                     GVIALCTHGRGGLRHRLFGSIAQQVIRLGTTPVLLVPAGGGPDPAAFACRSILVPLDG
                     DPDHEGVLPAVAALAARCAATVVLALVVPTFETLSGAMTAPSRLMPAATARLLDMSAQ
                     EAKNYLASPRRRLVGQGVRVSAHVFRGDPAAVVRRAAAAPGIDLVALATHGGTGSGAL
                     GTANLTARVCRSAGLPLLLVPVRTG"
     gene            27403..28509
                     /locus_tag="PKM94_05265"
     CDS             27403..28509
                     /locus_tag="PKM94_05265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD66085.1"
                     /translation="MKVPYLDLQAQYQSIKDDIAAAVQRVLDSSTYVLGPSVREFEEA
                     FARYCGTAHCAGVNSGTSALVLALRALEIGPGDEVVTAANTFVATVAAIAHTGAHPVL
                     VDIDPRTRTIDPRLLPMALSRRTRAVIPVHLYGRPADMEAIMRLAEKYEVSVVEDAAQ
                     AHGARYRGRRIGSFGRLAAFSFYPGKNLGAYGEAGAVTTDDPELDRRLRMLRDHGSER
                     KYYHDLVGYNARMEGIQGAVLGVKLRRLDQWNEARRRVAARYDELLRDLPVTTPPVDP
                     EVEPVYHLYVIETERRDALQAFLAERGVPTLIHYPVPVHLQRGYDFLGRRKGDFPVAE
                     RQAEQILSLPIFPEMTEAQVDYVAAQIQAFFHGA"
     gene            28499..30688
                     /locus_tag="PKM94_05270"
     CDS             28499..30688
                     /locus_tag="PKM94_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66086.1"
                     /translation="MERNAGFSPRLEVIAALALLALVALLRSYQLGADPPLGLSISTG
                     EQTDPPQYTLFARNYVQTGDFDPFDDARRAVFLKSSVTALAVAVFSLLGAGLWQAHLV
                     GLLLGLAALVLFWLFMRKVAGPLAGLLFLFLAAFNYNLIFFGRLPFLEQALVFWAFLA
                     AALLAYGRRPAIAAAAGASLALGIYFGKAIGLVFLFPFACWFAYEILVGRRERGRWLL
                     RLAAFAAGFLVVLAGWYLWVFAPAQAQVAGYYSEQIVDLYGAPEGLQSVDDFFFKLVT
                     FGATTRLFPRMRSAALLSAAFLLIMLFHVTRRTSWREGFGRLAAGHVFVAAMIVAFYG
                     ALMIWNYRPLRYQVVLIYPIYGAAAVVLAGLWRPWGVPSRRSVPLLFYPLAVPIVMVP
                     VYQIWDSLSEHYGGGLEWADARVWVVAASAVIVAAIALGLRFRPFGGRPGTQTAARAL
                     VGAVLLGTTVLGISNYHNWSVRPTFTARDVSRDLAMTLGPGAVVSGPYAPLLAAENNL
                     PVVVHMFGVAKADPGLFGRFPITHLLVDEANEAKARADYPAIMDSAAHVVTYHVGTDQ
                     VRLYRIAGRTPNPAANAYRPSLLERAIDAYHGGRTGEGHAWAAQFLERHPRNLSAYLA
                     VGEIADLEGQFDLAEESLKGALAFSPTSYNLNARLAELYRERFDETGDPELRHKGLLL
                     YEEAIRLAPSVAKLKSACNQLKTGRTEPEEEVDSPAPAGADSANLEN"
     gene            30806..31777
                     /locus_tag="PKM94_05275"
     CDS             30806..31777
                     /locus_tag="PKM94_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD66087.1"
                     /translation="MPKPSARFPFFVSTIVPACNEAGNIEQFCRLFADMLQSAPFDGE
                     LVYIDDGSTDDTLDKIKAAAAAYPFVRYVSHQHRRGVTDALRSGFSAARGDVFVFYPA
                     DLQFLPEDIPALVAPIADGADLVAGWKQGEYQKRFVSTVYNWLSRKIFNLKVHDLNAC
                     KAFRRGVVEQIFMRKDWHRYLVVLAANEGFRVEEVKVRLYPRAWGRSKYQSIWRVPVG
                     VLDMLAVKFQLTFLRKPLMFFGFTGLGFFLLGFLVGLWAVYLKYWRGETQLPLLYLVL
                     LLVGLGIALFAMGFITEGQAAIKEELASMRREVESRLRQLSRDRDHG"
BASE COUNT         5135 a        10984 c        10333 g         5370 t
ORIGIN      
        1 tggacagcac catgccgccg atgcgaaacg ccggcaccac cgccgagcgg gtgcgcgcgg
       61 ttccccccac cccgccgatc ggccgatcaa cggaggcgag gttggtcagg accgtgtcga
      121 gacggtgatc ctgaaaaaac cgatcgtgca gcaccagccc gaccgagttg ccgaggtcga
      181 tgatgaagtc tccttcgacg ccgccgaccg acgccgaaat cgtcgggacg agcatggtca
      241 ggtgaaaatc gaccgccgtt ccaccggcgg gcgcggtgaa ggcagccggg ttgtacgcgg
      301 tcagcgtcga atcggagtag tcaatcagca ccggaaaacg ggacaggaag tcatggccca
      361 ggataccccc tgcctgggcg cccggcggcg gttcgatccc cagccggtcc cagtccatcg
      421 ccgcggcaat ctggtccacc agggtgagcg agccgatcgt aatggagtcg gtccgcacca
      481 gagggacttc ctcgaacccg cctacccctt tggcggggac cgacccgacc gatgtcagtc
      541 ccagcggcgc ggtcagcgca tcgttgagca ggttggccga ggccccggaa tcgaggatga
      601 accaggcgac cgtcgcgccg ttgaccgcgg cgggcagcca cacgtgcccc cggtagtacg
      661 tgaaaggaat caccacggaa tcggcgcctg cggcgaagcg gtagtcgacc ggttccgccc
      721 ccggcaggtc gaacagcgcg ggatcgacct cgacgttgaa cgccacgctc tcggttgtca
      781 tctccgtata taacgccgcc tcggggaatt cggtccgcgc ggagtgcgcc agcgccaccc
      841 ccgcaaccgt ccggtaatcg gacagccgcg tcacgcccgt gaggttatcg agccgctgca
      901 cgacgtacgc cggcagggcc gactgcccgt ccagcagcat gacgaccgtg tcgtccggcc
      961 ggggatagag ggcgacccga agccacgagg tgtcggcgat cgtcgtgtcc cccagaaact
     1021 cggagcggcc gggcagacga ccggagatga taaatgaata cgaggccagg tagacctgtt
     1081 caatcaactg gcgcttctcc atcccttcca cttccgacac ccggccgttg tggtcgcgct
     1141 gccaggcggt ccggccgtca aaggctgaca ccagcgtcat cccgccgatc cggatttcca
     1201 tatagaatcg gtccggggcg gcatagacct gagtgtaagt cccctccaga ccgttgatat
     1261 cggccttccc ctccgtccgg taggaggtca tggcggcgag cgtgcggtac gcctccggcc
     1321 cgccgaccga ctgcaccagg agcgaatcca catcgaccgc tccggccgcg gccgcgacga
     1381 caactgccgc cgctgtcagc accaggctgc ccataacggt ccgttgtctt gtacacctgt
     1441 ccacgatgca cgtatacgct aaatcggcgc cgggcgaaag ctaaaatgtc gccgggaaaa
     1501 tcgaatccgg aatggtgccg gtgcggcgat gattttcccg gaagatccgc ggcccgtcgg
     1561 acggccgggg atgatgccgc gatctgtcag cgccgctgcc gaacggggga agactgcccg
     1621 cccgccggtc gtgtgaccgg ccccctccga tcgtcagttc tggatatact tgcccagaat
     1681 ctggttcatg cgcgacgtca tctgggagtt cggatacacc tcggtggcgc cggccacctt
     1741 ggccgctttc atctggtcct tcagcaggtt cggcgaataa cacacgatcg ggatctgctt
     1801 ggtggcgccg tacgagcgga gcttgttgat cagcgccggc gcttcaatcc cgttcagatt
     1861 caggtcgatg atgatgagat caggctggag gtcctgggcc ttgctgaaga tttcctccga
     1921 tctgatggct ttgatcccct cggccttgag agaatccagc gccgtcatga tcttggaggt
     1981 gaagatcatg ttgttggcag cgataatgac tctcttactc actgtgtcaa tccattgtta
     2041 cggtttcatc cctaacccag ggaagagcca tccccggtaa cccctgaccg gggcaatata
     2101 cactatccgg cgggcgaagt caaaccggga gccgtagaaa agtggtaact ggttgtcagc
     2161 cagatagatc acatgccgcc ggtggcaccc tccgccatcc gtgatgccct cgaccttccc
     2221 cgcccgtctg cggctgcgcc tttttgttga tttcccgatt cttatcccgt actaagaagt
     2281 tcgtgaaaca tacgaagata ctggccacct tcggtccagc catcgacgat cccgatcggc
     2341 ttcgggagct cgtctcggcc ggcgtcaacc tgttccgagt caactgctcc catggcgagt
     2401 ccgccgattt ctgccgggcc gccgccacga tccgccaggc caccgcccgg gcttgtttcc
     2461 cgatcggcct gttgttcgat atttccgggc ccaaattgcg cctggatcgc tttgagggga
     2521 gcctcgacat tgccgagggg accgagctca ccctggtcgc cgaccggaca gacctggccc
     2581 atcgactgat cgcggtcaac catcccgccg tgattgcctc gctgcgtgtc gacgaccggc
     2641 tgttcatgga tgacggtcag tttctctttc acgtgaccgc acggactgaa caggccgtga
     2701 ccgtgcggtc gcacagctcc ggaaagttat tgggcggcaa gggaattaac cttcccggcg
     2761 tgcccctgcc gattcccacg atcaccgcca aggaccgcca agatatcgag accgcggtgc
     2821 gctgcggcgc cgactatatc gcgctctcgt ttgtgcgctc ggccgacgat gtcgttcagg
     2881 cgcgggaact catcgccgcc gccggcggtg gcccgaaggt tatcgccaag ctcgagaagc
     2941 gcgaggcgat cgaccggctc gacgagataa tgtcggcggc cgacggggtg atgatcgcgc
     3001 ggggggatct cggcgtcgaa ctgccgccgg aggtcctgcc ccgcctgcag cggcatatca
     3061 tcaaggtcgc gaacaagttc cacaaaccgg tgatcgtcgc cactcagatg ctcgagtcga
     3121 tgcggttcaa tccgcggccc acccgggcag aggttaacga cgtagcctcg gcggtgctcg
     3181 actttgtcga cgccgtcatg ctctcggcgg aaacggccac cggcaaattc ccggtggagg
     3241 cggcccgcat gatgacgcag gtgatcaccg ccaccgagga ggccctcccc gccgcaagtg
     3301 ccgtccgggt cgagcgaacg gtcaccgatc ccgtcccgca ggcgatcgcc gacgccgtca
     3361 gcttcgttca ggagcaggga accgcccgcg tcgtattcgc ctttaccagc tccggcttca
     3421 ccgcccagct catctcaaac ttgttttcgc cgtatccgat tatagctttg actttgaatc
     3481 gctgcgtgat gtcggccctg tcgctccacc gctcggtgta tccggtgttg gtggagcagc
     3541 cggcttcgtt tgatgagatc gcgcgtctgg tgagcgcgat cggccgcgcc tacgccggcg
     3601 tgcggccggg cgaccgcgtg gtagtgaccg gcggtgcgcc gttcggccaa atgaaaccga
     3661 caaatttcat gatgatcgtg accattctgg acgaaaggga atccgggtag agaagtatgt
     3721 ggcacatgtg cagccggctg tgccggcact ccgggcgggc cgccgttgtc gtcctgctgc
     3781 ttgccggtct gagtttcacc tcggccgagg cgaagggcga ccacgagatc tgcatcacct
     3841 ttgacgagct cccggccgcc caggggttct cgtccaccgc gcccgaccag gtcttgcggc
     3901 cgatcctcaa ggcgctcaag aagtacgatg tcaaagcggc cggcttcgtc gtcggccaga
     3961 accttgaatc ctcattcgat ctgctcggcc aatggctcaa cgacggccac tgcctgggca
     4021 atctcacgta ctcctacagc gatctcaacg aggtcggcgc ggtcaatgtc gaagctttca
     4081 tcgccgacat cgtcgccggg cagcaggcga tcgagccgat gctcgagggg ttcggtcagc
     4141 agcgccgcta tttccgctac cccttcctcc actacggcac ctccctcgaa atgtaccgcg
     4201 ctgtcgccgg gtacctcgac gccaacgaca tcgcggtcgc ccacgcgagc gtcatcgtcg
     4261 atgactacct ctacgacctc gggctgcaga aagtcggcgg ccggccgacc gacaaagagt
     4321 acgaacagct catgaacgaa tacgtcaacc atgtcctcga tcagatcgaa gccgcggagg
     4381 tgttggccaa ggagatactc ggccggccct gccgccagat cctccagttg cgcgcgaacc
     4441 agctcaacgc gatgtacctc gacgtcatgc tccaggcgat caaggacatg ggctacgagt
     4501 tcatttccct cgaccgggcg ctccaggacg aggcctacca gaagccacac gcgtatttcg
     4561 gcggcaaggg ggtcgggtat ctcgagatga tcgaccgaag cgacccggac ttgctcccgg
     4621 cgggcgagtg acagtcactg cggcgcggga ggttgggtac agccggcccg cgccgcgcgg
     4681 acgggaggag gttttatgcg cagggcaagg tcattcctgt tggttctgtt ggcgacgctg
     4741 ccggccgccc cggccggcgc cgatccgctt gaccgccccg gagaggcggc tctcagcgcc
     4801 ggcatcacgt gggccgaggg ggatgtgtcc cgctacggtc tccgcgcctt cagcgtgctc
     4861 ggccgggtca ctttgcgccc gaaaccgatg agcatcttcg ccctctggtc caatctcagc
     4921 ggcaaccttt tcaacagcga ggaggaggtc gtgagtgtgc cgaccgacgg cgggtacatg
     4981 ccggccaccc gctcgaccac cgagactgcg ttcgccctcc acctgggcgt ccagctcaat
     5041 ccgcccgccg cccgcagccc gatccgcccg agagtcgcgt tgggggcggg cctgtggatg
     5101 ttcaacagcg acatcgccta ccgcgtcgac cgcgaggacg acccgttcct gtccgatgac
     5161 gagaccagtt tcgaattcgg tctccgcggc aacggcggga tcgacgtgtt cttcgccgag
     5221 aagtggggaa ttgcgctcga tttcaccttt gaccgcctgt tcggggtgga ccgggtggtt
     5281 ccaaccgacg atgccggggg cggccgcccg gcggcgcaga ccgcccgcta cttctcggcc
     5341 gcgctcgggc tggtcgctcc gctggaccgg ctcttctgac gcctcgcgcc gggctgagcg
     5401 gaggccccga aaaaagctgc ttttttttcg cgcggggagc ttgacggccg ggcggcagag
     5461 cgtattttgc gccccggaaa tgaccgcagg ccgcccgcgc gcctgcgccc ggtcgcccga
     5521 ccgaaccttt taaggtgatc cggtgaggtc accaaaggat tgacggagtt gaacgccgaa
     5581 caaggatacg cgccgccggc cgattccccg cgccgcccgc gccgcgcacc acggagcatg
     5641 cgccaaccct atccactgtt gccgacatgg aggtttcatt gcctgcacag acggatctaa
     5701 ttctcgatga gaagaaaatc gcccgcgccc tgcagcggat cgcgcacgaa attgtcgagc
     5761 gcaacgccgg ggccgaatcg ctcgtcatca tcggtgtgct cacccgcggc gcctttctcg
     5821 cccgccgcat cgcccggcac atcgaggaac tggaaggcgt caaagtcccg gtcgggctca
     5881 tggatatcag cctctaccgg gacgacgtgc actcgaaact cgaccagccc atcgtccagc
     5941 agacccagat cctcttcgcc gtcgtcgacc gcaacgtcat cctcatcgac gacgtgctct
     6001 tcacggggcg cacggtgcgc gccgcgctcg atcagatcat cgatttcggc cggccgcgct
     6061 cgatccagct ggccgtgctc gtcgaccgcg gccaccgcga actgcctttc cgccccgact
     6121 acgtgggcgt gaatatcccc accgcccgcg acgaccgggt ggttctggaa gtgaaggaga
     6181 aggaaggtca ggaccgggtg tacgtgatcc gcgccggggg cctgaagagc cggccggcgg
     6241 ctcccgggac agcgccggcg aagaagaccg cacgcgcggc cgggaaaaaa ccgaaaggga
     6301 aggggagcgc caaatgagcc gactgagttc caaccacctg ctgggactcg aaggggtccc
     6361 gcgcgccgac atcgaactga tcctggatac ggccgacacc ttccgcgagg tgctggaacg
     6421 gacgatcaag aaggtgccga ccctgcgcgg cctcaccgtt ctcaacctct tctacgagcc
     6481 gtccacgcgc acccggattt ccttcgagct ggcggagaag cggctctcgg ccgacaccat
     6541 cgcctttacc aaggccggca cctcggtgtc gaaaggggag tccctccgcg acacggtgca
     6601 gaatatcgag gccatgaaga tcgacatggt cgtggtgcgg cacagctcgg ccggcgcgcc
     6661 ctacttcctg acccagtgcg tcgacgccaa catcatcaac gccggcgacg ggtcgcacga
     6721 acaccccacc caggcgcttc tggacatgta cacgatccgc aagaagtacg gccggctcga
     6781 cggtcttcgg gtggtgctgg tcggggacgt gcggcactcg cgcgtgatcc gctcgaacat
     6841 ctgggggctc aagaccatgg gcgcctcggt cgccctctgc ggaccatcca ccctcctgcc
     6901 gcgcgagatc gagcagttcg gttgcgacag ctacaccgat ctcgatgagg cgctcgacgg
     6961 cgcggacgtc gtcaatgtca tgcgcatcca gctcgaacgc cagcaggccg gccttttccc
     7021 gtcccagcgg gagtacacca acctctgggg catcaaccgt gagcgcctca aacggctcaa
     7081 caagaactac acgattatgc acccgggtcc gatcaaccgc ggcgtggaga tcaccagcga
     7141 cgtggccgac ggcgaccggt cggtgatcct cgaccaggtc accaacgggc aggcggtccg
     7201 catggccgtc ctctatctcc tgcgcggcaa ggacgagggg gaggtcgagt aatgccgaaa
     7261 aaatcatacg atctggtcat tcgcggcggc cgcgtcatcg accccgcgct cggcttcggc
     7321 cgcgaagcga gcattttcat caaagacggc aagatcgtca agatggaaaa gacctccgag
     7381 gggtcgcgcg acctgaaagg catgcccgag gagaaagtca ttgatgcggc ggggatgatc
     7441 gtcacccccg gcctgatcga cgtgcacgtc cacctgcgcg agccggggcg cgaggatgag
     7501 gaaacggtcg agaccggctg ccgggcggcc gccgccggcg gcttcacctc gctctgctgc
     7561 atgcccaata ccgccccggc catcgacaac caggaaaccg tcaagttcgt ccaggaccgc
     7621 gcccagtcgg ccgacgcccg cgtgtacgtg gtcggcgcca tcaccaaggg acgcaaaggg
     7681 gccgagctct ccgaaatcgg ggatctcgtg aaggtcggcg cggtggcggt ctccgacgac
     7741 ggcgcctacc tgcagaacgc cgagatcatg cgccgcgccc tccagtacag ccgcatgttc
     7801 aacatcccgg tcctccagca cgccgaggac cagcagctgt gcgagggcgc cgtcatgaac
     7861 gaatcgttcg agtcgaccaa gctcggcctg aaaggggcgc cgcccgaggc cgaggagatc
     7921 gccgtgctcc gcgacatcgc cctcgcccgc tcgaccggcg gacgggtgca cttccagcat
     7981 atcacgacca agcgggcgat cgaggccatc cggcaggcca agaaggaagg gatccgcgtc
     8041 accgccgagg ccgccccgca ccacctcgtc ctcaacgacg cggaaatcgc gaaagcgttc
     8101 aataccaacc tccgggtcaa cccgcccctc cgctcggaga gcgaccggca ggcgctcatc
     8161 gaaggcctcg tggacggcac gatcgactgc atcgcctccg atcacgcacc ccactccgag
     8221 gaggagaaag actgcgagtt cgacctggct ccccccggta tgatcgggct ggagacttcc
     8281 ctcgcactca tcaacacgtt cctcatcgag aagggtctga tgaactgggc cgacgtgatc
     8341 cgcaaaatga ccgtcaaccc ggcccacatc ctgaaactcc ccggcgggac gctggcggtc
     8401 ggttcctccg ccgacatcac gatcatcgac ccggagaaga agtggacggt gcgggccgac
     8461 aagttccggt cgaaatcgaa gaactcgccc tatatcgggt ggaagctgac gggccaggtc
     8521 gcccggacca ttctcggcgg ccgcacggtc taccagcgct aaggtgcggc gcaggacgcc
     8581 ccgggggcgg cggttcgccc ccggcagtcc cgcgcggggc cgcgcgtgcg gtttcccaaa
     8641 atgaccttgt gatcggggcc gcgcccgcgt acctttgacc cattgacagc ggatcgcggc
     8701 ggcccggccg cccggtccga tcggcatcca ccagcaagga ggcgaaatgc cgaccttaag
     8761 tcgcagccgc atggcgcgga tcgctgcacg gacgctcgca aacatggccc gccgccggcc
     8821 catcaatgtt tcgttcgaaa tcacctacaa ctgcaacgcc cggtgcgaac actgcgacct
     8881 cggcgactat gtcaaggaac cccggctcgg cccggaggtg ttcgccgact ggctgacccg
     8941 cctcaaaccg gcagtcgccc agatctccgg gggagaaccg ctgctgcgca aggacctcgt
     9001 cgacatcgtg cgcgccatgc gcgagcgcga cccggtcccc gtcttcgtca tcaccaccaa
     9061 tgtccagatc atgaccgaga agaaatacct ccagctccgg gaggccggca tggacgagtt
     9121 ctccttctcg ctggactatc ccgacgaccg ccacagcgcc ttccgccacc tcaaggacaa
     9181 ctttcgcaag atgagcgagt tggtcccgac gctggcggcc cacggccaca aggacatcgt
     9241 gctcgcctgc gtcgtgcagt cggacaactt ccgcgacctt ccgaaaatcg ccgagctcgc
     9301 gcgggaatgg ggggtggggg tgaatttcag cacctacaac gctctgcgca ccgggaagaa
     9361 gtactacctg atcacgtcgc cgcaggatct cgccgagctg gagacgattg tcgaccggct
     9421 ggcggcgatg cagcgggagg gctacccgat catgacctcc gaatggacga tgcggcaaat
     9481 gatcgacttt ttccgtaccg gcggccaccc ccgctgccgc gccggggaac ggttcatgat
     9541 cgtcaacccg tgggggaagc tgaccccgtg cggcatgtgc cgcgaccact acgatgatcc
     9601 ggcgcagatc cgcgagcggt tcagccgcac gaatgagtgc ggcgactgct acacggcgat
     9661 ccgcgccaat tccgaaaaaa ccccctaccg gctgattgcc gatgccctcc gggtggtgcg
     9721 gcgctaggcc cggcactctc ttaagcgcga cgtccgcgcc gcccactccc gggtgagccc
     9781 tatgccgaca ccggcgccga aagcccctac cgtcctcggc cacctcggcc tcttctacgc
     9841 cgccgctatc tggggggcga cctttttcgt cgtcaaaggg gcgctcgccg ccgtcgatcc
     9901 cctcatcctg gtcgggtacc gcttcctcat cgccggcggg ctcctgctgg cggttgtgat
     9961 catcctcgga cggaacctca cggcccatct cggacggtcc gccatcctcg ccgtgattct
    10021 ctggaccctc tacatctcgc agacggtcgg cctgcagtac accacggcct ccaactccgg
    10081 cttcatcacc ggtctattca tcgcttttgt cccgatcctt ctgagactgc tctttcgccg
    10141 ccggccgacg atcatggagg ttatcgcttc ggcggtctcg ctggtcgggc tgtggattct
    10201 caccggggga ttgcgccagg tcaatgtcgg cgacatgctc accctcattt cggccatgac
    10261 ctacgccctg cacctgctgt attccgacaa gtatctcaag gagggcctgg atccttgggt
    10321 gatgagctgc cagcagtttc tcctggtggg cgcactgagc attgcggcgg ggtgggtgac
    10381 cgggcggccg ttcgcggtgc ggacggccgg tgcgatgtgg gtggtgatct tcctggccct
    10441 gctgccgacc gcctcggcgt tcgtgattca gatgctggcc cagcgcatca tcgcgccggt
    10501 gcgggtgtcg ctggtttttg cctttgagcc ggtgtttgcg ggggttttcg cctggactct
    10561 gggcggggag ccgttcattc tccgcacggc cggcggcggt ctgctcatat tcctcggcct
    10621 cattctctcc gccttgcccg atcccccggc tttccggccg aaacgcgcgc ccaccgcccg
    10681 ccggcgggct acttgaggag gagcattttc ctggcggcgc agcgggaggg ggtctccagg
    10741 cggtagaggt agacgccgct ggccgccggc cgccccttcc cggttgtccc gtcccagacc
    10801 gcctcatgcc gtccccccgc ctgctcccca tccaccagca cccgcacctg ctgcccgagc
    10861 agattgtaca ccgtcagctt cacccgcacc gtccggggaa gcgtgtaccc gatggtcgtg
    10921 cgcggattga acggattggg gtaattgggc gtcagggcga atccgtccgg cagcgtctcc
    10981 tccggcggca gagcagtcgc cgtgccggtc aggtcattga ccgcccggtg caccgccgcg
    11041 atcgccgccg cctcgtcgaa gaacgacaag ggcaggtcaa accagatgta gcggtagtcc
    11101 gcccccaggt accgccaggc caccgctttc ccctccgtca gcgtgtcatc cgtgcccgaa
    11161 tcgtaggtgt agaggacatc gggcagggaa gagttcagcg tcacaaacga ccccgcgcag
    11221 atgccgaaag gtgtctggat cgagggctcc tgctgcgccg tccgcagcga atcccaccgc
    11281 agcgccggcc agcccgcctg ctggggatgg gccccgacca gatccccgga gacctggcct
    11341 cccgccacca ggggcagcac cgaccgctcg gcgaggtgga acacggtgct gtaggcgtcg
    11401 tcccaggcgg ccgcggtggc cgcgtagggg atggtcacca ccgtgttgcc cgaactcgga
    11461 ccgaaccggc cgaagacgag cagcttcccg ccgatcgcga ggtagtaggc cagggtgtcg
    11521 agcagtccgc cccccgccgg gctgaaggcg acattgtcgt agaaggtctc tccggccacc
    11581 accaccagtt cgtagctgac gaggtcgtag agccgggtgc gggaggaggc gtcggcgtag
    11641 gcggaatcgg agcggtagtc gcaggcggcg ccggccaggg cggcgcggag gaactccccg
    11701 gtgagcgcga cctcggctcc gggaaccgcg acattgtcgc ggttgatcgc caatatccgc
    11761 cccggcgtca aggtcgccgc ccgggcctcg aacggcgtcc ggccgatcgt gtcggagaca
    11821 atgccgtcgt catccacggc cgcgacaagg tactcatgga actcctcccc cagccccgga
    11881 tcctggtcct gaaaaaccgt gtcggaagtt gtgcccgcaa gcgcgccgtc gcggtagatc
    11941 gcgtagtggc tcaaatccag ttcctggttg ttccgacccc actgcagcgc cacgccctga
    12001 tgaatcggca gtcctgtcag cccctccggc ggcgccggcc gcgactgcgg gatgccgatc
    12061 ccgtcgggcc acgtcagcga ggtctgtccc gcggaatcga acgcctgcac gtagaagatg
    12121 tacgcggccc cttcggtcag cccctccacg gtgtgtatgc tgtcggaagg agggaggtcg
    12181 agacactgga gggtcgcggg gcttccctgg cggtaccagc agaggcggta gtgatcgagc
    12241 gcggtccggc ggagggggcg ccactccacg aggagcgacc ggccgtcgcc cggctgccgg
    12301 atcgcggcta tcggcaccgg cggcggcagc gtcgccgccg catacacggc ggccagcgac
    12361 acgcgcacga tccgctccac gtaatcgaaa ttgagatagg tcgtcgaatc gctcggcgag
    12421 tggtagtggg tggagaagtc gtcctcggtc agactgagga agtcgtagcc gttgctcccg
    12481 aacggcacgt ggtccgatct cccgccgtac ccggcgatat ccccgacgag cggtccggta
    12541 gtcggggcga ggagcaccca cagatcggcg tagaccgaat cgtggttgaa atacacgcgc
    12601 gcgaccgaat cgttcgtctc gtgcccgatc atgtcgaaat taaccatgag gacgatctcg
    12661 cggttgagcg ccgccatcga atcggcgtag tggcgcgagc ccagcaggcc gctctcctcg
    12721 gcgtcgaacg ggatatacgt cagagtcacg ggcgccgcgc acccggcgag aacgcgggcg
    12781 agttcgagca ccgccgccgt ccccgaggcg ttgtcgtcgg cgccggggct gcttgccacc
    12841 gcgtcgtagt gcgcgccgat cacgatctgc tgttcgggat atgccgcgcc cggccggcag
    12901 gccaccacgt tgcagcactc gccggggggc gtgctcgatg aggggtaggg atcgaaatag
    12961 tcgagcacca ccgaatcgta gccgaacgcg gcaaacttag ccgccagcca gtcccgcgcc
    13021 gcccggctcg agtcggtgtc ggacacgcgg cggtagaatg cctgcagccg ctcggtgtaa
    13081 ctgcgcagcg agtcctcgct caccagggcg accaggctgt cgattgtatc catggcgccg
    13141 gcggccggct gcggcgccgg gcgcgcgggg gagtagcgga tcggcacggg gaagtcggtg
    13201 atcggaacca gctcggggtc cttctcggcg gcggcgcgag aatcaacctg caccagccgg
    13261 agtcgatcgc gcgcaaacac caccgcgtgc ggtccccggt acgcgccggg ggcgctgcgg
    13321 tcgatcgcca gacggtcggc tgcgacgccc tgggctacga cagcggcctc cacgccggcc
    13381 gcggtcaggc gggccaggcc cgagtcggag atcaccgcca ggtacaagtt ccccgccgcc
    13441 gccaccgccg tcgcgccggc ggcctcgagc tgctgggcct cggcaaaatc ccgaagcgtc
    13501 acttccacca atttgtcggc gactaccggg gcggcgccga gcatcaccgc cgctagcgtc
    13561 aggaatccca tgcggaacgt catatccact ttcctccaac gccaccgccc gccggcgggc
    13621 tacttgagga ggagcatttt cctggcagcg cagcgggagg gggtctccag gcggtagagg
    13681 tagacgccgc tggccgccgg ccgccccttc ccggttgtcc cgtcccagac cgcctcatgc
    13741 cgtccccccg cctgctcccc atccaccagc acccgcacct gctgcccgag cagattgtac
    13801 accgtcagct tcacccgcac cgtccgggga agcgtgtacc cgatggtcgt gcgcggattg
    13861 aacggattgg ggtaattggg cgtcagggcg aatccgtccg gcagcgtctc ctccggcggc
    13921 agagcagtcg ccgtgccggt caggtcattg accgcccggt gcaccgccgc gatcgccgcc
    13981 gcctcgtcga agaacgacaa gggcaggtca aaccagatgt agcggtagtc cgcccccagg
    14041 taccgccagg ccaccgcttt cccctccgtc agcgtgtcat ccgtgcccga atcgtaggtg
    14101 tagaggacat cgggcaggga agagttcagc gtcacaaacg accccgcgca gatgccgaaa
    14161 ggtgtctgga tcgagggctc ctgctgcgcc gtccgcagcg aatcccaccg cagcgccggc
    14221 cagcccgcct gctggggatg ggccccgacc agatccccgg agacctggcc tcccgccacc
    14281 aggggcagca ccgaccgctc ggcgaggtgg aacacggtgc tgtaggcgtc gtcccaggcg
    14341 gccgcggtgg ccgcgtaggg gatggtcacc accgtgttgc ccgaactcgg accgaaccgg
    14401 ccgaagacga gcagcttccc gccgatcgcg aggtagtagg ccagggtgtc gagcagtccg
    14461 ccccccgccg ggctgaaggc gacattgtcg tagaaggtct ctccggccac caccaccagt
    14521 tcgtagctga cgaggtcgta gagccgggtg cgggaggagg cgtcggcgta ggcggaatcg
    14581 gagcggtagt cgcaggcggc gccggccagg gcggcgcgga ggaactcccc ggtgagcgcg
    14641 acctcggctc cgggaaccgc gacattgtcg cggttgatcg ccagtatccg ccccggcgtc
    14701 aaggtcgccg cccgggcctc gacagccgcc gcggtgtccg acaggttgcc gtcgaagtcg
    14761 accgccgcga ccaggtagcg gtgcagctcg gttcccagcg cggggtcgcg gtcctcgtac
    14821 acgctgtcgg cggtcgtgcc tgcaagcgtg ccgtcgcggt agacggcgta gtgctcgaaa
    14881 tcgagttcgt gatcggtgtg gcgccaccag acccggaccc cgtgagagat cgggagcgcc
    14941 gccagtccgg gcggcgcggc cggccgcgaa tcgggccgtc cgagcacctc ctccaccgcc
    15001 agcgccgtcc gcccctgcac gtcgtacgcc cgcacgccga agacatacgt gactccttcg
    15061 gtgagcccgg tcaccagcgc gctgctgtcg ccggcgggga gggtgaggtt gtgcgcgttt
    15121 cccggagaac cgtccgggta ccagtacacc cggtagtggt cgatgttggc gctgtgaatc
    15181 gcgcgccact ggaccagttg cgactggccg tcgcccggct cccggatggc cgtgatgcgc
    15241 accggcggag gcagcgtcca gactgtcgcc gcggtcgcca ggctcacttt caccatgcgc
    15301 gtcatgtatt cgaaattgag gtgggtggtg ctgtcgctgg gctcgtggta gtgggtcgag
    15361 aagttgcgct cctggacgaa aatcacgtcg tagccgttct ccgcgaacgg caggtggtcg
    15421 gaggccgtgc cccccatcag gctcgcggca attccgccgt aggtcggcgc caaatccgcc
    15481 cagagcgccg cgtaggactg gtcggctccc cagtagaggt tcgctccggc ggtgttggat
    15541 tcatgcgcga tcatgtcgag gttgatcatg aggacgattt ccgccccctc ggccgccacc
    15601 gaatcggcgt agtggtacga tccccacatc caggactctt ccgagtcgaa cgcgatgaat
    15661 ttgagggtca tggggaagtc cccggcggcg agcacgcgcg cgatctccat cgtgcccgcc
    15721 gttccggtgg cgttgtcgtc ggcgccggga caatccggca ccgcgtcgaa atggccgccg
    15781 atgatgatct cctgctccgg gtagagcgcc ccgactttgc acgccacgac gttgtagctc
    15841 ggcaccggga cccggtccca gagctgctcg ccgacaaagg gatcgatcgc caccgagtcg
    15901 tagccgaagg ccaggaaccg ggcggcaatc cagtcgcgcg ccgcggcgga ggaatcggtg
    15961 ccggtgaggc ggcggtgaaa ggcctgcagg cgctcggtgt acgaccgaag cgaatcttcg
    16021 ctgatcagcg cgaccaggct gtcgatggtg cccatggcgc cggcggccgg ctgcggcgcc
    16081 gggcgcggag ggacgtagcg gaccgcaacc gggccccgga cgagcggcgc caggtcggcg
    16141 gcgtgcgggc ccgcgagggc cgagcggttc ggcagagcga cgaggcgaaa ggcgtggcgg
    16201 tcatacacgg ccgggtagcc gggccgggca tctcccggcg gacggcggtc gaacgccagc
    16261 agaggccgcg ccacatcaaa ggcgaccggt tcgatccgca cccccgcctc gaccagcttc
    16321 ttccactccg cgcgctcccc cagcgcgagg tacacgttgc ccgtcagcgc caccggggtc
    16381 agcgcagatt cgcgcagcgc ccgggcgtca cgatggctgc cgattgttac ttcgaggaga
    16441 tcttccgccg cggccgaggc cgccaggcag atacagaatg ccgctgcgag cacccgactc
    16501 ataggtctgt tccctattga ggttccttct actgccaata tacgaaaaca caacggccgc
    16561 gccgcagacg gttgtgaaaa aaaacgcccc gaaccggccg gcgcagactc ggcttttttg
    16621 ttctctcccg ggccgccccg cgctaagttg agaaaaagga tccgtcgatg attcagatat
    16681 tcggcacgcg aaaatgcccg gacacccgca aggcggaacg gttcttccgc gagcgcgggg
    16741 tggctatcca ggtcatcgat ctgcgcgaga agggagtgag tccgggggag ctgcgcagcc
    16801 tcgcccgcgc cgtgcccctt tccgaactga tcgacacgga gggcaaggag tacgagcggc
    16861 tgaacctgag atacatccgc cacaacgtgg aggagatgct cctggcccac ccccttcttt
    16921 ttcgcacgcc gattgtgcga tcaggcccgc gcgcggcggc cgggtatcat ccggaaatct
    16981 ggcagacttt cgccgaggcc gagaagtgag tcgcggcgcg cgggatcccg ccggtcaggg
    17041 acagggggac ggcgccggac cgccgcggaa caggtaggag atcaggaacg tcaggtccga
    17101 tacggtgatt cgcccgccgc tctccgtgct gccgttggcg ttggcttgct ccgggcacgg
    17161 gggcgtggct ccgccctgca gcaggtaggc aatgagggcc gtcagatcgc tcacattgac
    17221 ctgcgcgccc ggcaacccgc tgtcgttgac gtctccccgc accgcgcagc agcaggcgtc
    17281 gcccacgccg tcgccgtccg tgtccgtctg atcggggttg tggtcctcgg cgcagttgtc
    17341 cgcccacgag ggcagcccgt cggcatccgg atcatcgagc accacggtca cggtgaactg
    17401 cgcggtatcg gcattgcccg cggcgtcctg cgcggcgacc gtcaccacgg tgctcccaag
    17461 cgggaacagc gatccggagg gcggctcggc cgcagccaga gccccggcgc agttgtcgag
    17521 cgcggcgatc gcgaaatgga caaccgctcc atacaacccc gaatcgctcg tcacctcgac
    17581 ggcctcggga cactggacgg tcggcggttc ggtgtcgtgc acggtcactg tgaaaaaggc
    17641 ggtatcggca ttgccggcgg catcgaccgc caccgcccgg accgcggtcg ccccgacggg
    17701 aaagaacgaa cccgcggacg gctggagcgt caccgcggga aggccgcagt tgtccgacgc
    17761 cgcggcctcg taggcggccg cggccccgca ggctccggag tcgttcggca gatccagatc
    17821 ggcgggcgcc gtcaccgcgg gcggctcttc atcccgcacc gtcaccgtga accgcgcggt
    17881 gtcgacgttg ccggcggcat cgaccgccac cgcctcgacc gtggagactc cgaccggaaa
    17941 gtacgatccc gagggcgggt tgaccgacag cgcggcgctc ccgcagttgt cggccgccgt
    18001 caccgcgtac gcgaccatcg cgctgcacac tcccggttcg gcgggcacca tcaggtcatg
    18061 gggcgccgtg aggcgcggag gctcgtcgtc cacgaccgtg atgtaaaagg ctgcggagtc
    18121 gacgttgccg gagaggtccg ccgccgccgc cgtcaccgcc gtcgtgccca gcgggaagag
    18181 cgagccgggc ggcagcgaca ccgtcaccgc cggcgtgccg cagttgtcca ctgccgacag
    18241 ggtgtaagtg acggccgcgg cgcactggcc gctgtcgacg ccgaccacca ggctctccgg
    18301 gtagatcaac tgcggcggct cggtatcgcg aacggtgacc ggaaaccagg tcgtatcgca
    18361 gttattcagg aggtcggtgg cgatcacctg caccatcgtt gtccccaccg ggaagaccga
    18421 tcccgatggg gggtaggcga ccgccgtggc gagcgggcag ttgtcggttg ccgttaccga
    18481 aaacgtcgcc ggggcgctgc attggccggg tgcggcggcg accatgaccg agtcggggtg
    18541 atgggccacc ggccattgcc ggtcctccac cgtcacccag aatgtgtcga ctttccggtt
    18601 gccggagtag tcgacggcgg tgacgatcac gtcggtggcc ccgacggaga agtacgaacc
    18661 ggggggaggc gacccggacc aggtcacgcc cgggcagtta tcgtacgtcg cggcgctgta
    18721 ggtccacacc gtgccgcact cgttcggggc attgccgatg accgtatcgc gcagggcagc
    18781 caggatcggc gcggaggtgt cggtcaccgt aaccgtgaac ccgcaggtgt cccggttccc
    18841 ccaggcgtcg acggcaatgc actgcacgta gttggcgccc cggtaaaaat acgtgccgga
    18901 gggaggggtg cacgagatgg tcgctcccgg gcggttgtcg gtgacggtgg cggtgaactc
    18961 caccactgcg gcgcactcgc tcggcccggc cgccaccgtc gtatcattcg ggcagtgcgc
    19021 caccggcggc tgaaaatcct gctgaatgag actgaccccg ccgctgtaga aatccagaat
    19081 ccgcccgccc gccaccgagc tgcaccagct gggcgcaccg tacggggtgg gaaaggccgt
    19141 atcccgcgtg atctcccact ccgagatccc atcgaaaccg tagacatcgt ccgacatgta
    19201 gagcgtatcc ccccagcgcg aggacacggc gttgtcgccg cagtcgtacc agatgtattt
    19261 cacgggcagg aaccggccgt agaggctgta atcccagggg atgtcgaaag tgatgtccac
    19321 cagatccgcg gttggcatcc cgaagcacga ggggtggtgg gcgccgttgg atatgtcggc
    19381 gatcgcggtg atgtggatat tgttgacgcc gatcaggttg gaggtgaaat actcccaccc
    19441 gcagagatta agcatctgcc cccggttgac cgcgatcagc gtgagcgtgg tgtcgtagcg
    19501 gaccgtcatc tcgaatccgc ccatctgaaa agcccccgtg ctgtcgacgg ccgccagggt
    19561 cagcgtcacc cggtcgcccg ggcgcgccaa ctccgaccgc tcgatccgca tcgctatatc
    19621 ggcgagcgcc cggcccgcga ggctcgctat gacggctgcc gcaacgacga gtactttcat
    19681 ggacgcatac acccagctac accctaatct aacggattcg tctccgctgt caaacgtcga
    19741 ttcccgacgt tgccgaatct aattgcacgc cgcgacgtac tctagtatca atcggctgcg
    19801 gacgggagct tggttactcc cgccgcggcg gaagtggagg cgcgtatcgg cggcccgcca
    19861 gccggtcagc atcggcgaac ttctcatgat ggattctgcg gcctacatcc aaaccgacga
    19921 ggtctgccgc tactatcgac gcggcgccag cgaagtccgc gcggtcgacc gggtcagcct
    19981 ggccgttgag aggggcggct tcctcgccat cgtaggggct tccggttccg gtaagtcgac
    20041 attgctcaat ctcttagccg gtcttgacac gcccacctcg ggacacatcg cggtcgccgg
    20101 agccccgctc ggggcgatgt cgcggcgcga gctgtcggcc taccgcgcca accgggtggg
    20161 ggtcgtcttc cagaccttca acctgattcc ccatttaacc gccctccgca acgtcgagct
    20221 ggcgctctgt ttcaacggta cgccgcgccc cgaacgccgc cgccgggccg ctgcgatgct
    20281 cgaccgcctc ggcctggccg accgggccga ccaccgcccg gctgatctgt cgggcggcga
    20341 gcagcagcgg gtcgcgctgg cccgcgcgct ggtgaaaggc ccggagattc tgcttgccga
    20401 tgaaccgacc ggcaacctcg accgcgagaa cacgcggcag ttggcggacc tcctgaccgc
    20461 acacaatcgc gacgggctga ccgtgatcct cgtcacccac gatctcccgt tggccgagcg
    20521 gctcgccggc cgcgtcgtgc ggatgcacta cgggcggctg gtcgaaggaa ccgaggccgg
    20581 atgatgaccg tccgcgacct cgtcgagatc tccgccggca atctctggcg gatgaaactg
    20641 cggacttgcc tcaccctggc gggtgtcgtt atcgccatcg gcgccttcgt cgccatggtc
    20701 tcgttcgggg ccggcgcgga gaagaacgtc tccgaggagt atgagaaact cggtttgttc
    20761 acgaccatgc aggtctaccc gccggcggcc gacagcctcg gcgactcggt gccgccgccg
    20821 ccgctgaccg actcggtcgt cgcccgcctg gcgcgtctgc cgggcgtgcg cctcgcttat
    20881 ccctacgacg ccttcagcgt ctccgtccgc ctcgacacgc tcgagctctc ggctgaggct
    20941 caggtgcttc cgccggcggc cgtcgcgacc cgtctgtatt cccgtctcgt cgcggggcgg
    21001 cgaccggacg ccaacggctc gcgcgaggcg atggtgacgc gcgagtttct ggaagatcac
    21061 gcgctcggac ccgccgactc gatcctcggt cgatcgatcg ccgtctccgt ctcgtcctcg
    21121 acgctcgaca gcgccgtggt ccacgtcgtc cgccaggaga gccggcgcgt cgaacaattc
    21181 atcgacgcct ttgacttcga ttccctcgac aacgtcgggt accttcggcg gacggcgacc
    21241 ggcgaattca acgccgcggc gcggagcttt ctcgaggggt atctcgggcg gccgatgatt
    21301 accaccgaca cgctgaccat tgtcggcgtg atcgacggcc cgcagggccg ccggctccgg
    21361 gtcaaacccc tcatcattcc ggagcaaagc gcccggcgct tcgccgcggc caagagcggc
    21421 ccggacccgg ccgaactgct ggccgccttc cagagcgggc gcgtcggcaa cctcttcggc
    21481 ggcgacacag cggcgcccga cgcctacagc cgcgtcacgc tcgacctcga accggggacc
    21541 ggctacgccg ccctgagcga cagcatcagg gcgatgggct acgagacctt cagctacctc
    21601 gagcagttcg ccgagatcca gcgcttcttc ctctatttcg acctcatgct cggggtggtg
    21661 gggatgatcg cgctggtaac cgcctccctg gggatcgtca acaccatggt catgtcgatc
    21721 ctcgaacgcc gccgcgagat cggcgtgctg aaatcgctcg gcgccgacga ccgcgacgtc
    21781 tggctccagt tcctcgccga atccggcgtc atcggctcgg tcggcgccgt cctcggcatt
    21841 ctcctcggtt gggttgtgac ccgcatcgct tcgttcgtgg ccgtgcgggt gatggagaac
    21901 cagggaatga cggcggtgga attgttcgat ctccccctgt ggttgatcgc ggtcgcgctc
    21961 ctgttcggca cggcggtgag cgtcgccgcc ggcctttacc ccgccgcgcg ggccgcccgc
    22021 gtcaatccgg tcgaagccct ccgcaatgag tgagcccgct ccggagaagg gagacgtctg
    22081 tggaccgacg atcccgtgag gcattcgcag ccggccgaac cgcgctgctt tcggaagcgg
    22141 cgcgcgagtg ggattttgag ccgcgcacgg ccgccgaact ggatggcttc gagaaccaca
    22201 tctttgaggt cgcgcgcggc gggcgtccgt tcgtgctccg tctgtgccac tcgctccacc
    22261 gtacggccgc gctcgtctgc gctgaattgc actggctgga cttcctggcc cggcgcgggt
    22321 tgcccgtggc cgcgcccgtt cccggccgcg gcggcgccct cgcccgcgtg atccccgccg
    22381 ccgactcctt cttcgttgcc tcactctgtg cgaaagcccc cggccggaga ccgctccgga
    22441 gcgactacag cgaccgcttc ttcgaaaact ggggggagct ggtcggccgg atccaccgcg
    22501 tcacccggga gtacctgccg ctcgcgccgc cggtccggcg cttccactgg catgaggacc
    22561 cgacgctcga cccggcgccc tatcgcccgg ccgtcgacga cgacgtcctc gcccgcttcg
    22621 cccggatcgt ggcgcagctc agggcgctgc cggtcgatcg tacggcctac ggtctgatcc
    22681 acaacgacct tcaccccggc aatttcttca tcgacggcga ctgtctgacg gcgttcgatt
    22741 tcgacgattg ccactaccag tttctggtca atgatgtagc gatggccctg ttttatgcct
    22801 tgcccttgcc cgctcggccg aggaccgccg aggcgttcgc ggagacgttc cttccgccgt
    22861 tcctccgcgg ttaccgccgt cacgccgcgc tggggccgga ggacctgcgg cgtctcccga
    22921 tgttcctcaa actccgcgag ctcgacctct acagccagat tgtgcttcac ctcggagccg
    22981 acgtcggcgg ctggcccggc gcgtacctcg aggggcggcg cgaccgcatc ctcgccgatc
    23041 gcccggtcgt cgacctggat ttcgcggaat tttgacccgg gccccggtgg gcggcagccc
    23101 gtcctatccg gcccgggtgt ccgcctgttg cccgaatcgc gcccacggcc tccgatgcgc
    23161 ggcggccatg atgacgcagc cgatcaggag cagcaacccg ccgagcgcct cccgcgccgt
    23221 cggcaactgg tgcaagagcg ccagcgcgat gagcatcacc accaccggct ggctctggct
    23281 gatcaccgcc accttggtga gttccaaccg ggccagcgcc tgcaggtaga acagccggga
    23341 gacgagcggc ccgaacagcg ccagggcgat caccccgggc cacacctggg cgagtcccgc
    23401 cagatcgccg cccgcggcga acagcaccgc ccagtacccc agcgcgagca gtccgttgcg
    23461 gatgtatgtc aggatcagcg ggtcgacatg ccgcaccgcg tgcttggaaa tgaactcggt
    23521 gaggccgaaa ccgatcgacc ccaggagcac gagcccgaac ccgaccgagt attccccccg
    23581 cagcgtcagc cgcatgagcg cgatcccggc cagcgacagc agcaccccca gcgtctcgat
    23641 ccggctgagc cgctccccga ggaacagaat gccgagcgcg atcgccacca gcacctccga
    23701 ccggttgagg aaggaggcca gcgatggatc catcttctgg acgccggccc acaccaggag
    23761 gacggcgaac caggacgaga ggctgaacag gagaatccag aaccaggcgc gccgggtctg
    23821 ccggaaaacc cgcgccggac cgtagccggg cagcacccac gccgacaaca tcaccgtcgc
    23881 cagcgagaaa atcagcgcgt tcatggccag gggggagagg tgcacaaggt tccatttccc
    23941 ggtcgccgtg gccgccccgc ccccaaccgc cgaaatcagc gccaaaaggt aaccggtgcg
    24001 ccgttgcgcc gcaagcgcgc cggccggtcc ggggcggctc ggcttggtcg gcatgacggc
    24061 tacagccccg gtgggggcgc gacaaagggg gtcaggggac catcggggcg ggcgagcgcc
    24121 gccaggtcgg tataggggac cgtgtagccg gtcgggccca attcctcctt gcccgcctcg
    24181 ccgcggttga catagaaggc caagcctttc ggggtgatga ggaagtttcc gttgtgacgg
    24241 aaaccggccg tggaatcgaa cgcaaacccg gccgcggcca gcgactccac gagatcgagt
    24301 ctgcgggcgc gccggaacgc ctgctcggcg gttcggttga gaagtgtgtc gccgccggga
    24361 atcagcaggt cgctcagggc gagcagccgg cccgtccggg cgtcgaaatt gacataccgc
    24421 cgctccgcat gctggtcctc cggaccctgg taccgggtct ccaccaggct cactcccaag
    24481 atgccgagcg tgtcggtctc cacccgggct ttccgcaacc acgtccagcc gtagctggct
    24541 gtccgcgcgg gatccggccg gtccttgaaa ttccggatga actccttggc catgtcgtcg
    24601 accgtctcga acgtgcccgc tcccgccatc ggccgcagga ggaactgcca gacgcgcgcg
    24661 gagaccgagt cggcgacccc tctctccgag gctgccgtga cctccgggta ggtcacgttg
    24721 aggtaggcga aaccgttgac cgtgtctccc tcggtgccga accgccgctc gatcgttttc
    24781 atctcatacg tgattgtggg gaagcggtcc tggtcggaac cgcaggccgc gggcaggagg
    24841 gccgccgcgc tgatcgagag aatcgcgtaa tgtcgcgccg ccatgggtga tacctcgagt
    24901 tggttccgcc aaggtagcgc aaactcccgg taaattcaac ttctttgtgg gaaccgccga
    24961 tggggggtgc ggcggctgcg gtgcgaagag aatcttgcgc ctgcgggaga ctcctgcgta
    25021 cgtgttgtcg gcgggcggcg cagccccgaa agtaacgcgc ccgacccgat ttcgacagcg
    25081 agcgaaatat gaccgacgcg ccatccgaac cgcccgacga gattgtcccg aggaagccgc
    25141 cgcccgtgct ctcggacgac ctgacgcggc ggatcgccga gcaggtgctg tcgggggagc
    25201 gcgtgtcccg ctggcagcgg ttgctgccgt tcctcgggcc ggccttcatc gccagcgtcg
    25261 cctacgtcga tcccggtaat ttcgccacca atatccaggg cggcgccgag ttcggctacc
    25321 gcctcctctg ggtgatcgtc ttctccaatc tcatggccat gctgctccag acgctctccg
    25381 cgaaactggg catcgccacc ggctacaacc tcgccgagca ctgccgccgc gagtttccga
    25441 cctgggtcgc ctgggggatg tgggtgctca tggaagcggt cgccatgtcc accgacctgg
    25501 ccgaattcct cggcgccgcc ctcgggttct acctcctgct cggcatcccg ctcatctggg
    25561 gcgcggtgct taccgggatc gcgaccttcc tcattctcgg tctggagcgc cgggggttcc
    25621 gccgcctgga ggcggtcatc acggccatgg tcgccgtcat cgtcttcgcc tatgttgtgg
    25681 aatcgatcat ggaccggccg gactgggggc tggtggcctg ccatgcggtc gtcccgagcc
    25741 ttcccggccc ggaggcggtg ttgctggcgg ccgggattct cggcgcgacc gtgatgccgc
    25801 atgcgatttt tctccattcg gccctgaccc agggccgcat ccaggttcgc ggcgccgccc
    25861 agctgcagcg gctctttcgt ttccaaatca tcgacgtcac cgtcgccatg gggctcgcca
    25921 gcctcgtcaa catggccatc ctcatcatgg ccgcggccac tttccatcgc cacggcctgt
    25981 ccgagatcgc ctccatcgag gaggcccacc ggactctcga accgcttctc ggcagcgccg
    26041 ccagcggcgt cttcgccgtg tcgctgctgg cctccgggtt gtcctcctca actgtcggta
    26101 cgagcgccgg ccaggtgatc atgcagggct tcctgaggcg ccacatcccg gtctgggtgc
    26161 gccggctggt gacgatggcg ccggcgctga tcgtcatcgc cctcgggctc gacccgacgc
    26221 ggacgctggt catcagccag gtggtcctga gtttcggcct cccgttcgcc gtcgtgccgc
    26281 tcgtgctgtt caccgctcgc cgctcgatca tgggcgtgct ggtgaaccgg aaaccgacta
    26341 ccgtcgcggc cgcgatcgtg gcgtcgctca tcatcggcct gaacgtgttc ctcctgtacc
    26401 aactgctttt cagggagtga gctgtgtttg aacgcgtgct ggttcctctc gatggttcgg
    26461 cggtggccga gcaggtgctc cccgccgttg ggtgcttcgc cgaacgcctc ggcgcggcgg
    26521 tgacgctcgt gcacctgatt gaacggcggg cgccggccga aatccacggc gagcgccacc
    26581 ttcgcacggc ggaggaggcc gagcagtatc tcggcgcgat cgcccgccgt atcgccgccg
    26641 ggcggctggc ggtccgcacc cacgtgcaca cggtggaggt cgccagcgtg gcgcagagca
    26701 ttgtcgagca cgccgaggag ttcgcgagcg gcgtcatcgc cctgtgcacc cacgggcgcg
    26761 gcggtctgcg gcaccgcctg ttcggcagca tcgcccagca ggtcatccgc ctcggaacca
    26821 cccccgtgct cctggtcccg gccgggggtg gaccggaccc cgccgcgttc gcgtgccgct
    26881 cgatcctcgt gccgctcgac ggcgatcccg accacgaggg ggtgttgccc gcggtggcgg
    26941 cgctcgccgc gcggtgcgcc gcgaccgtcg tcctcgccct ggtcgtcccc actttcgaaa
    27001 cgctctcggg ggccatgacc gcgccgagcc gcctgatgcc ggccgccacc gcccggctcc
    27061 tcgacatgtc ggcgcaggag gcgaagaact acctcgcatc cccccgccgg cggctggtcg
    27121 gacagggcgt gcgggtaagc gcgcacgtgt tccgcggcga cccggcggcg gtggtccgac
    27181 gggcggcggc ggcgcccggc atcgatctgg tcgcgctggc gacccacggc ggcacgggga
    27241 gcggggcgct cggaacggcc aacctgaccg cccgcgtttg ccgctccgcc ggtctgcccc
    27301 tcctcctggt ccccgtgcgc accgggtagc tccccgcgat cccatccgcc ttgacttttg
    27361 ccccggcgtc gggtactttg gcatcctacc aaaggaagca tcatgaaagt accctacctc
    27421 gacctgcagg cccagtacca atcgatcaaa gacgatatcg ccgccgccgt ccagcgcgtg
    27481 ctggacagtt ccacttatgt cctcgggccc tcggtgcgcg agtttgagga ggcgttcgcc
    27541 cgctactgcg ggacggcgca ctgcgcgggc gtcaacagtg gcacgagcgc cctcgtgctc
    27601 gccctccgcg ccctcgagat cggtcccggg gacgaggtgg tcaccgccgc caacaccttt
    27661 gtcgcgaccg tcgccgccat cgcccacacc ggggcccatc ccgtgcttgt cgacatcgac
    27721 ccccgcaccc gcacgatcga cccgaggctg ctgccgatgg cgctctcccg acggacccgc
    27781 gccgtcatcc cggtccacct ctacggccgc ccggccgaca tggaggcgat catgcgcctg
    27841 gccgaaaaat acgaggtgag cgtggtcgag gacgccgccc aggcccacgg ggcgcgctac
    27901 cgggggcggc ggatcggatc tttcgggcgg ctcgccgcct tctctttcta tcccggcaaa
    27961 aacctcggcg cctacgggga ggcgggggcg gtgaccaccg atgaccccga actcgaccgc
    28021 cgcctgcgca tgctccgcga ccacggaagc gagcggaagt actaccacga cctggtcggc
    28081 tacaatgcgc gcatggaggg gatccagggc gcggtcctcg gcgtcaaact tcgccgcctc
    28141 gaccagtgga atgaggcccg gcgccgggtc gcggcccgct acgacgagct gctccgggat
    28201 ctcccggtga ccaccccccc ggtcgatccc gaggtcgagc cggtctacca cctttacgtg
    28261 atcgaaaccg agcgccgcga cgcccttcag gccttcctcg ccgagcgggg cgtcccgact
    28321 ctcattcact acccggtgcc ggtgcacctg cagcgcggct atgacttcct cggccggcgc
    28381 aagggagatt tcccggtcgc cgagcgccag gcggagcaga tcctgtcgct cccgatcttt
    28441 ccggagatga cggaggcgca ggtggattac gtcgccgcgc agatacaagc gttcttccat
    28501 ggagcgtaac gccggtttca gtccgaggct cgaggtgatc gcggcgctgg ccctgctcgc
    28561 gctggtggcc ctcctgcgca gctaccagtt gggggccgac ccgccgctcg gcctgtcgat
    28621 atccaccggc gagcagaccg acccgcccca gtacacgctg ttcgcgcgga actatgtcca
    28681 gaccggggat ttcgatcctt tcgatgatgc ccgccgggcg gttttcctga aatcgagcgt
    28741 caccgccctc gccgtcgcgg tcttctccct cctcggcgcc gggctgtggc aggcgcatct
    28801 ggtcggcctg ctcctggggc tggccgctct cgtgttgttc tggctcttca tgcggaaagt
    28861 cgccggcccg ctcgccggcc tgctcttcct ctttctcgcg gccttcaact acaacctcat
    28921 tttcttcggg cggctgccct ttctggaaca ggcgctggtg ttttgggcgt ttctcgcggc
    28981 cgcgcttctg gcctacggcc ggcgaccggc catcgccgcc gccgccggcg cctcgctcgc
    29041 actcggcatt tacttcggca aagcgatcgg cctcgtcttc ctctttccct tcgcctgctg
    29101 gttcgcctac gagatcctcg tcggccggcg ggaacggggg cgatggctgc tccggctggc
    29161 cgcttttgcg gccggtttcc tcgtcgtgct cgccggttgg tacctctggg tgtttgcccc
    29221 cgcccaggcg caggtggccg gctactacag cgagcagatc gtggatctct acggggcgcc
    29281 cgaggggctg cagtcggtcg acgatttctt cttcaagctc gtgactttcg gcgccaccac
    29341 ccggcttttc ccgcgcatgc ggagcgcggc gctgctctcc gcggcgttcc tgttgatcat
    29401 gctgttccac gtcacccgcc ggacctcatg gcgcgaggga ttcggccggc tcgccgccgg
    29461 gcacgtcttc gtcgccgcga tgatcgtggc cttctacggc gcgctcatga tctggaacta
    29521 ccgccccctc cgctaccagg tggtgctcat ctacccgatt tacggggcgg cggccgtggt
    29581 gctggcgggc ctctggcggc cgtggggggt gccctcgcgg cggtcggtcc cgctgctctt
    29641 ctatccgctc gccgttccga tcgtcatggt gccggtgtat cagatctggg acagtttgtc
    29701 ggagcactat ggcgggggcc tcgaatgggc cgacgcgcgg gtgtgggtgg tcgccgcgtc
    29761 cgccgtgatt gtcgccgcca tcgctctcgg ccttcgcttc cgcccgttcg gcgggcggcc
    29821 cgggactcag acagccgccc gggcgctggt gggggcggtg ctgctcggca ccaccgtgct
    29881 cggcatcagt aactaccaca actggagcgt gcggccgacc ttcaccgccc gcgacgtgag
    29941 ccgtgatttg gcgatgaccc tcggccccgg cgcggtggtg tccggcccct acgcccccct
    30001 cctggccgcg gagaacaacc tccccgtggt cgtccatatg ttcggtgtgg ccaaggccga
    30061 ccccggcctg ttcggccgtt tcccgatcac tcatctcctg gtcgatgagg ccaacgaggc
    30121 aaaagcgcgg gccgattatc ccgcgatcat ggacagcgcc gcccatgtcg tcacctacca
    30181 cgtggggacc gatcaggtgc gcctctatcg gatcgctggc cgtaccccca atcccgccgc
    30241 gaacgcctac cggccctcgc ttctggagcg ggcgatcgac gcctaccacg gcgggcggac
    30301 gggggagggg cacgcgtggg ccgcccagtt cctcgaacgc catccccgga acctctccgc
    30361 ctacctcgcg gtgggggaga tcgccgacct tgagggacaa ttcgatctgg ccgaggagag
    30421 cctcaagggc gctctggcgt tttcacctac cagctacaac ctcaacgccc gcctggccga
    30481 gctctaccgg gaacggttcg atgaaaccgg cgaccccgag ttgcggcaca agggcctcct
    30541 gctgtacgag gaagccatcc gcctggcccc gtcggtcgcc aagctcaagt ccgcctgcaa
    30601 tcagttgaaa accggccgga ccgagcccga ggaggaggtc gactccccgg ctccggccgg
    30661 cgccgactcg gcgaacctcg aaaattagcg ttgagtttca ccggccgatt ggcgatgttt
    30721 tgagtatgca ggatgcttta ctcaggttgg gagatcgcgc acccgggacc gccttcagcc
    30781 ggtagcccgc ggtgggggtt agtgtatgcc gaaaccgtca gctcgctttc cgttcttcgt
    30841 ctccacgatt gttccggcct gtaacgaggc gggcaacatc gagcagttct gccggctgtt
    30901 tgccgacatg cttcagtcgg cccctttcga cggtgagttg gtctacatcg acgacggttc
    30961 gaccgatgac acgctggaca agatcaaggc ggccgccgcg gcttaccctt ttgtccgcta
    31021 tgtttcgcac cagcaccggc gcggcgtgac cgacgcgctc cgctccgggt tcagcgccgc
    31081 gcggggagat gtgtttgttt tctatccggc cgatttgcag tttctccccg aagacatccc
    31141 ggccctggtg gcgccgattg ccgacggcgc cgacctggtg gccggctgga aacagggcga
    31201 gtaccagaag aggtttgtct cgacagtcta taactggctg tcccgcaaga tcttcaacct
    31261 caaggtccac gatcttaacg cctgcaaagc gttccgccgc ggagtggtcg aacagatctt
    31321 catgcgcaaa gactggcacc gctatctcgt cgtgctcgcc gccaacgagg gtttccgggt
    31381 cgaggaagtc aaggtccgtt tgtacccgcg ggcctggggc agatcgaagt accagtcgat
    31441 ctggcgcgtc ccggtcggcg tgctggacat gctggcggtg aagttccagt tgacctttct
    31501 gcgcaagccg ctcatgtttt tcggcttcac cggcctgggg ttcttcctgc tcgggttcct
    31561 cgtcggtctg tgggccgtgt acctgaaata ctggcgcggg gaaacgcagc tcccgcttct
    31621 gtacctcgtg ctgttgctgg tcggtctggg gatcgctctc tttgccatgg gattcatcac
    31681 cgagggtcag gccgccatta aagaggagct tgcctccatg cgccgcgagg tcgagagccg
    31741 gctgcgtcag ctcagccggg accgcgacca cggctgagcc gttttcctgc cgtcatcttt
    31801 ccgggggcca tgattctcgg gg
//
LOCUS       DAOPOE010000026        31618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1867_length_31618_cov_6.739980, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000026 DAOPOE010000000
VERSION     DAOPOE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31618
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_31618_cov_6.739980"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKM94_05280"
     CDS             <1..816
                     /locus_tag="PKM94_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD66088.1"
                     /translation="IILGAAVIDDVMGLVILAVVTGVIAAVAHGGGEGITSLGILWIL
                     GKAVLFIAGAIVIGNYILPSVFRVAFRLRGHGMLLSVSLLVLFALSYAAGKAGLAPIV
                     GAFAAGLILEEVHFRRFTERGEHSLRELIEPIATFLVPIFFVRMGMMVDLTAFGRVEI
                     LGFAAMLTLAAIVGKQACSLAIFDRRTNRLAIGLGMIPRGEVGLIFAGIGLTLMLDGH
                     AVVSSETYAAVVIMVIVTTLVTPPLLIWSMHRGGTTRTEAPAEKSGAAGRPTP"
     gene            818..1681
                     /locus_tag="PKM94_05285"
     CDS             818..1681
                     /locus_tag="PKM94_05285"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66089.1"
                     /translation="MEIAPLTIAGLEFRLREEPGIEREWFTARLRDYLAYEPGGCFAL
                     AEGGETVGMVTALCYQTTAWLGWLYVAAARRGAGLGERLMRQGMGYCIARGMRSIVLE
                     AVVEAAPLYRRIGFQRQFRTQHYRLEKTRRGAAPSPSVAVRRFAADDLVRIADLDRRM
                     FGQDRRRVLDLVRRNPLHRGWLAEKEGKVAGYLWATKAAANRQAGPWVIDPGTGDAAE
                     TAAALCDAAFAESDRPLILRCPLVTESRARLWDDLGAQAIDYHTERMYAGEEYAPEGE
                     GVLCLGCPGKG"
     gene            1826..1899
                     /locus_tag="PKM94_05290"
     tRNA            1826..1899
                     /locus_tag="PKM94_05290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1860..1862,aa:Met,seq:cat)
     gene            2169..2594
                     /locus_tag="PKM94_05295"
     CDS             2169..2594
                     /locus_tag="PKM94_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66090.1"
                     /translation="MFVHNILAGRWHADYNIIKGNGHYRESRLSEETQMRDRTHRAFV
                     EVIRALSLPTICHDALLWSPDGDPVPPGPGRAGGGAPFGGIELFPAGTFGTDRPCAGA
                     AWYRAAGGRSLSFTASGLRSLALPPLFFLSIRFAQEVTS"
     gene            2594..3766
                     /locus_tag="PKM94_05300"
     CDS             2594..3766
                     /locus_tag="PKM94_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66091.1"
                     /translation="MTRARKHYLLLIPAIAFAAALGAGGCGTKEGVPAAGPPEVRVAV
                     AATKDVPVSREWLGQTLGAVDIEIRARVDGWLESIHFREGSEVRKGDLLYTIDPAELE
                     QKVVQAKAKVAEAQTMFVRAESDANRYRPLAEAKAVSQRDLEAVLAEYGARQGEVEAA
                     RAALRLAEIELGYATITAPIDGLIGLSAARVGDLVGRPPSPVILNTVSRVDSVRVRFS
                     ITENEYLELARRLQATPDGPPARERFPLRLILSDGTRYPHVGSVSVGQREIDPATGTL
                     QLEALYPNPERLLRPGQFARVVAEVGMRKDAVVVPARAVTELQGQQMLFTVGADNKVA
                     VRRVAVGPSAGPFTVIDRGLAAGEKVIVEGQQRVRPDMPVRPVEIPADSLAGGGGR"
     gene            3770..6958
                     /locus_tag="PKM94_05305"
     CDS             3770..6958
                     /locus_tag="PKM94_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOD66092.1"
                     /translation="MSKFFVNRPIVAMVIAIVMVLVGLIVMRGLPISQFPDIVPPMIN
                     IATTYVGASAIDVEQSVATPLELQVNGVEDMIYMKSINTSDGMMTLQVSFEVGADLDV
                     ANMLTQNRVSQAQASLPPAVKNYGVTTKKSLVFPLLLVALTSSEESYDNIFLSNYAAI
                     NINDRLSRINGVGDVKLFGGGDYAMRIWVRPDALARLGLTVSDLVRAVQEQNVISPAG
                     QLGAPPAPPGTEFTYTVRTQGRLFNEEEFGRIVLRTNPDGSQVRLRDVARIELGTLMY
                     GQTSRNNGKPAAVIGVYQLPGSNALAVEESVSKTMEELAQRFPPDLVHEITLNTTRAV
                     EEGINEIVHTLFEAVLLVILVVFLFLQNWRATLIPLIAVPVSLVATFMVFPLLGFSIN
                     TLSLLGLVLAIGIVVDDAIVVVEAVMHHIEEGLPPKEATLKAMEEVSGPVVAIALILT
                     AVFIPVALIGGITGQLYQQFAITIAISVLFSAFNALTLSPALAAKLLRPKGQGRRSPL
                     QRFYDWFNRGFGRFTGGYLSVTGILVRKVMRGVVFIGILLAATALLLKGVPTGFVPEE
                     DNGYFLITVQLPDAASLERTDAVCRKIEAILGETRGVANYTAVAGYSISSQAPGSNVG
                     TFFVSLEPWEERTTGEESSFGLVAALNQRFAREIPGAAVFAFGPPAIQGLGTGAGFTF
                     MLQDMTGGSPQELAAMAEQFAAAARQRPEIGRVATFYRAGVPQMYAEVDRDKALKLGV
                     SIADVNTTLGSLLGANYINDFNRFGRVYKVYLAAEGEYRNETWDLGLFYVRNKNGEMV
                     PLSTLVTMKPVSGPQYTNRFNLYRSAEMMGGPAAGYSSSQALAALEEVAADVLPAGWG
                     YDWANMSYQEKKAAGTGGVVFVMALVFVFLILAAQYESWSLPLSVLLATPFAIFGAMT
                     GLWVSRMVDTTYVNNVFAQIGFVTLIGLAAKNSILIVEFAKMQKEKGLPTVEAALAAA
                     KLRLRPILMTSFAFILGVVPLVLAAGAGAEARKVMGMAVFAGLLVATSLGIFLIPMLY
                     VVVQKYFVRERSPEPARRCDDGAGAAEKGAGV"
     gene            6955..8406
                     /locus_tag="PKM94_05310"
     CDS             6955..8406
                     /locus_tag="PKM94_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOD66093.1"
                     /translation="MMKFLMAGFSAALVAGCAVGPNYVRPEIETPGRWQNPDSVFAAD
                     TAAVRSETAWWGLFGDTVLTNLIAAALQENADVRIAAARVEEVMGRYGVARSDFFPKL
                     GAAGAASRGQLLSDEAGRGLESTRNRFQVNLAADWEIDLWGKLRRATEAARADLLATE
                     EARRGVVLTTVALVASTYVDLLSMDWQLEIARQTALTRREALDLFEVRREKGDLSDLI
                     FSQAEAEYWLAMSQIPLIEENIAFLENMISCLVGRNPGPVARGKTLEDLAPPAVPVGL
                     PSELLERRPDIRFAEEQLRAANARIGVARALYFPSLSLSGLLGYASDDLSDLVNSDTR
                     TWRVGGDVLAPIFRFGEIKGQVKAAEGRQKQALYAYVEAVHNAFRDTENALVRRRCTN
                     EQLQAEGKRVAALATYARLARMRFDEGVTSYLEVLDAERALFATVLAYTQTTADLHKS
                     VIALHQALAGSWLDAAAAESFRIEEGIKLRGQP"
     gene            8504..9334
                     /locus_tag="PKM94_05315"
     CDS             8504..9334
                     /locus_tag="PKM94_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66094.1"
                     /translation="MDSNRTTCMLAAVMRAVIVAGAALLLLGGSPAHAEHWFFGVGTG
                     FTFMNIQGEQGYTVINYGPVQYDLDLDPDDISDVLESAFGFAGYATDGTWIIQYSLGV
                     FTLGGDGRVDLPPEVGGGTLDVDVEFNVTNGKITLGRTVYRDKKMKFSFTPYAGLRYL
                     RHEINADFVVTQGDESTPIFRGRDYKWTDALVGATISYRLTPRFTWTASGDAVFGGSD
                     GTFYFTTSLPFTPTPHFSFGPNLSYTAIDYENDDKGDADWYLYDADEFGAGIFLAVNF
                     "
     gene            9496..10770
                     /locus_tag="PKM94_05320"
     CDS             9496..10770
                     /locus_tag="PKM94_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017635362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOD66095.1"
                     /translation="MLDRYDAPHLAVSRGSRRRFHCMIKAIGSACNLGCAYCFYLSKA
                     HLPGGPGRGRMSQEMLARLIQQYIEGVTGDEVVFSWQGGEPTLLGLDFFRRVVALEKQ
                     FARPGQRIENDLQTNGTLITEEWCEFLKENRFLVGLSIDGPRELHDTYRVTRTGRPTF
                     DRVFRAAMLLRRYGVPYNTLTCINRMNARRPLDVYRFLRHEVGSTYMQFIPVVEYRGF
                     ELTAPHRWHNDALPRDGEAAARPGHPDSIVTDWSVDPEDWGYFLCRTLDEWLNRDFGK
                     VLVNHFETLVSQRLGRGSQLCLYGEFCGKGVAVEHDGAVFACDHYVYPEYRLGSIGET
                     SLVDLVFSRAQARFGYAKSETLPPWCRDCPYLTDCWGECPKNRIIRTAGGEPGLNYLC
                     RGFKKFFARAIPEVERLAAALRRSPQQPRKRM"
     gene            11441..12985
                     /locus_tag="PKM94_05325"
     CDS             11441..12985
                     /locus_tag="PKM94_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOD66096.1"
                     /translation="MPAQAQTAPAQGQKPNILVIFGDDIGWSNISAYNMGMMGSRTPN
                     IDRLAKEGAIFTDYYGQQSSTAGRAAFITGQHPFRVGLLKVGMPGAELGLQPEDPTIA
                     DLLKNEGYVTGQFGKNHLGDRNKFLPTVHGFDEFFGNLYHLNAEEEPEDPQYPKMPEF
                     IQQFGPRGALLCKATEVDDSTVDPRFGRVGKQTIKDTGPITRKRMETAENEFLNASLD
                     FMDRAVKANKPFFIWHNSTRMHVWTRLSPKYQDKTGYGLFADGMQELDDVVGALLKKL
                     DDLGIADNTIVVFTTDNGAEKATWPDGGATPFRGEKGSTWEGGMRVPCAIRWPGVVKP
                     GTVINGIMSHEDWTPTFLAAAGEPDVKDKLLKGHTANGKTFKVHLDGYDQREMLAGTG
                     PSKRQEIFYFDDNGNLNAVRVRDWKAHLALPGDWYQAGDPVPQTFPKIVNLRMDPFED
                     HVLFGESPMAARWMTDKFWAFVPMQGVVAQFLQTFKEFPPRQRSASFNIEQVMLQLQQ
                     SSTARN"
     gene            complement(13109..13732)
                     /locus_tag="PKM94_05330"
     CDS             complement(13109..13732)
                     /locus_tag="PKM94_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66097.1"
                     /translation="MRQRHALAAILSAALVAGCAVGPRVVDTWRDPAYTGPAFKKIAV
                     VGITDRESSRRIFETAFAATLREQGVAAVPSYDVYRTDEKMPREEIERLVRENGIDAI
                     ITARVVDRTQRIEITPATALAVPWPAHYRGYYGFYDYAWAFYHDPGYVTASDVVSIET
                     NVYETSGFNIVWTGTSRTTNPANLEEESKGLAGGIIRELISSKLLAP"
     gene            14072..14866
                     /locus_tag="PKM94_05335"
     CDS             14072..14866
                     /locus_tag="PKM94_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66098.1"
                     /translation="MPWNSPANQRRLRRLFDEGFSAMDIAEPLASFDDDRSAAAVRAF
                     LEQRDLMVAGVRRDGFVCGFADRRELGRGRLGAYLRPFPPECILSEADSLRKVILAMR
                     GGGPCFVSVLGEVGAVITLADLEKPPVRMFLFGMITIFEMIMTWGIRTYFPGDSWRAH
                     IPPGRLAKAEQLWQERQRRRSTADLLDCLQFSDRAQLLLRIPRVTDLLAQGGIPSRRA
                     ALTAIGELETLRNNIAHSQEIIPESWSRIVVFSTRLELLLGEAFRS"
     gene            14890..17193
                     /locus_tag="PKM94_05340"
     CDS             14890..17193
                     /locus_tag="PKM94_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66099.1"
                     /translation="MRSCKLAGAALLLAVLAWPLAAQDAPPVDGGWPRTFTTASDGEM
                     TLFAPQVAAWENQERLVAFCAVSYLPAGATERRLGTIKIHAATEVSLEERLVRMTNLQ
                     IVESNFPGLPKDQVRGLLAELMASYADTARVIALDRLLAAIDKSQLGPGNLDGVKAEP
                     PPLYVSTRPAVLVIFDGAPVWSPIKDNDLKFAVNTNWDCFAHAPTQTFYLRWEDSWLQ
                     APDAGGPWSPAGTLPGSFDKLPDDGNWNEVAAHLPGRALPAAEAPAVFVSTVPAELLL
                     LEGEPEYEAVAGTRLFWVRNTESDVFRLGTDGTVYLLLSGRWFSAPDFTGPWTFATPD
                     LPEDFRNIPREHERSDVLACVPGTDEAAEAVLLAGIPQTARVNKAELKPPEVVYAGDP
                     EFTPIEGTSCRQATNTDKDILRVGDLYYMCFQGVWFMSRTPTGPWEVTGDVPAAVYEI
                     PPSSECHHVTYVVVEHYDPADVYVTFSYTSGYTGVIIAWGVPVYGTGWYYPPFVYWGY
                     PYPVYYPRWVTYGYAVRYNPWTGIYAASARIYGPYGGARYGVAYNPRTGTCVRGAMAY
                     GPYGAAGAAAAWNPRTGAYAATRQGANAYGSWGESYVQRGDNWAHTARYTDAAAGQTK
                     RVTRTDEATVVNTRGPQGGGFIGAKGDDVYAGHDGSVYKRDENGNWSKYSDGGWTDTE
                     KPARAGERAKGEADRPSRDAVAPQAGKASSGTPSAASTLGQLERDSAARSQGARRADQ
                     YGRQRKQAATSSREAARPRSGSGGRRR"
     gene            complement(17309..18004)
                     /locus_tag="PKM94_05345"
     CDS             complement(17309..18004)
                     /locus_tag="PKM94_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD66100.1"
                     /translation="MLRRGTRLAALIILTAAAAEAKVDVELQPYGGLRTATALGEGYY
                     ENDLADRLDVAPGVSFGLTCDFPVGGIGPTGQQAMIELSFSYQRSDLRFEPASMDKVP
                     DVYLDRFEVKDDKLILGDIEVMYLHGGGLYRFGNYSGWLPFANFGVGATFFAAPDGGA
                     DSRTKFSFSFGAGAARMFSERLGARLGFRGYMTSLPAEEAYWRDPWGGVWSITDDNWL
                     FQGELSLGLVVRF"
     gene            18668..20122
                     /locus_tag="PKM94_05350"
     CDS             18668..20122
                     /locus_tag="PKM94_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOD66101.1"
                     /translation="MRCIPGVHTRTAIGLVCAAFFWLVGSVGWGQAGGEAEAETDPLV
                     RAKLEWFQDMKFGLLMHWGTYSQWEIVESWSLCPEDEDWCRRRGEQAGDYFEYKRAYE
                     DLQTTFNPTKFNPDSWAAAAKAAGMRYVILTTKHHDGFCMFDTRETDYKVTSSACPFS
                     ANPRANVAKEIFAAFRTQGFGIGAYFSKPDWHNPNYWWPYFPPFDRNVNYDIARYPER
                     WEAFKTFTANQIRELMTDYGAIDILWLDGGWVQPMTAASPRWGKHPCDQDIDMPRIAA
                     MARSLQPGLIVVDRAVEGRYQNYRTPEQEVPDTALDYVWETCMTMATSWSYVSTDTYK
                     PTSQLIRLLAEVAAKGGNLLLNIGPSPEGELPPTALARLAEIGAWMAVNGEAIYGSRA
                     VAPYGEGRVCFTRSPDGSINAIYLAGDGEHRPPDSIAIASFAPKAGSEVRMRGVEDPV
                     AWEPAAAGFVIHVPPSARVHPPCDYAWTFRFVIK"
     gene            20163..21494
                     /locus_tag="PKM94_05355"
     CDS             20163..21494
                     /locus_tag="PKM94_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOD66102.1"
                     /translation="MRILQIIAVVLSTMLVPLSGRGKQADQPKDESKMQWFADAKLGI
                     FIHWGIYAVDGVGESWSFHNRQLPYDDYMRQLAGFTASSYDPGEWAEAIGEAGARYAV
                     LTSKHHDGVALWDTKMNDLSVPRRTPAGRDVIAPFVEALRARGIKVGLYFSLIDWSHP
                     DYPGFLRDSSRYNAGAAPDRWRRFVQFYQGQLNELMTQFDPDLYWFDGDWEHSAEEWQ
                     AAGVRDLIRRHNPAAIINGRLQGFGDYDTPEQNLPIERPGSRCWELCQTMNDSWGYQG
                     RDTNYKTPSEIITIFADVISMGGNLLLDIGPQADGTIPEKQRNILRELGRWTARHAEA
                     IFGTVAGPPPGHFYGPATLSKDSLSLYLFLPGRTGGDVVVKGLNCGVAGVTVVGSGET
                     VTYRTVGKISWSPVPGLIFIHPPENQDEYMTVLKVSLDSPLALYRGKGGLQ"
     gene            21491..23743
                     /locus_tag="PKM94_05360"
     CDS             21491..23743
                     /locus_tag="PKM94_05360"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOD66103.1"
                     /translation="MKRRSVLLLPLVLACLLAGGAANVAPAPSLTLATDGRSEYRLCL
                     PPHASGQVTAAADTLVKYIREVSGATIPLSEGGAAEGPRIVFEIGGTEDPSAGIAGLG
                     DDGFAIRSEGDDLFLAAETPYGLQNAVYTFLETYLGCRLYCPAVRLIPRRSTLTLPAI
                     NDRQVPVIAFRMLNVRDSGFTTWHKLNRNDDFGLFVHTFRLLVPPDRYFPDHPEYFSL
                     LKGNRTASGQLCLSNPEVFRIVCEKLRALMNEKPGARFWSVSQNDTYVPCECSACRAI
                     DSAEGSPSGSILAFVNRVADEFPDMTISTLAYQYSRSAPARLKPRPNVNIMLCSIECN
                     RSRPLAEDPSSASFVKDVADWSRLTHNIFLWDYVINFRNLVSPFPNLRVLQPNIRFFV
                     QHGITSVFEQGLSGFHGEFAELRSYLIAKLLWNPDIDVDSVMDDFLQGFYGEAAPYIR
                     QYIDTMHAAMEASGEGLDIYGYPVRSADGYLSAAMLDLYGRLFDQAEAAVQGEPAVLA
                     RVRTARLPVQFAVLEQAKVAAAGERGCFVRAADGSLSVRPTIDSLLAAFVQGCREAGI
                     PALWEYGTTPDKYGAATRAFLESGTTTHLARGKAVRLGRPASPKYHDGDRAALTDGLK
                     AWDDYHMHWLGFEGEDMDATVDLGTVRTISRISTSFLQDINSWVFMPESVTFSVSTDG
                     RDYRLVGTVRDTVPADRGGKVVAPFNVAFAATNARFVRVYASSLKTCPTWHKGSGGPA
                     WIFIDEIAVE"
     gene            23754..26378
                     /locus_tag="PKM94_05365"
     CDS             23754..26378
                     /locus_tag="PKM94_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOD66104.1"
                     /translation="MKVLKFILLSVLACISAPAAPEAQPYRDPSLPVDRRIDDLLSRM
                     TPEEKFRQLFMLAENPGDSLERHAHGVFGFQIGGGEDAPVAVVANRIQRHFVERTRLG
                     IPVILFAEALHGLVQKGATSFPQAIALAATFDTALMHDVAATIAEECRARGIRQVLSP
                     VVNIASDARWGRVEETYGEDPFLVSAMGVAFVSEFERRGVITTPKHFIANVGDGGRDS
                     YPVHMNERLLREIFLPPFAACIGRGGSRSIMAAYNSYDGSPCSASNWLNNHLLKEELA
                     FPGFVISDAGGVGGANVLHFTAADYPEAGEHAINGGLDVIFQTSDNHAALFLPPFLDG
                     RIRSDVIDRAVRRVLRVKFELGLFEDPYVDTADGGLRQRRETGRELARRAAREAIVLL
                     KNDGNTLPLDSTPVRIAVIGPDAAEARLGGYSGPGNDPVSILEGIRARAGRAGAVTYA
                     RGCRRLDTAYVTVPSTVLSCVVRDSVRAGLLGAYYANAALTGAPSLVRVDPEIQFQWT
                     LFSPEPGLLPYDCYSAQWTGTLTAPASGLFRIGLEGSDGYRLYLDDSLVLDRRRQVSS
                     AARLAEFRFEQNRRYAIRLEFAEPAGNARLRLVWDVGVSTDGDSLPAEAVAAAAASDV
                     ALVVVGLEEGEFRDRVSLPLPGRQEELIRRVAATGTPVIVAIVGGSAVTMSRWIDSVA
                     AVLEVWYPGEAGGDAVADVVFGDVNPGGRLPITFPLAEGQLPLVYNHKPTGRGDDYFD
                     LSGRPLFPFGYGLSYTQFAYGDLRFDRGEIGIGDTGIVRFTVTNVGAREGDEVVQLYV
                     RDLLASVARPVIELKGFRRVHLKPGQTAMVTMEIPPEALTTLDERLRPTIEPGDFRVM
                     IGSSSTDIRLHGTLRVRP"
     gene            complement(26629..28566)
                     /locus_tag="PKM94_05370"
     CDS             complement(26629..28566)
                     /locus_tag="PKM94_05370"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOD66105.1"
                     /translation="MRAFVLPFLVWACTAGILPPALARGAPPVTHVISHDRTLAVTDP
                     STGSNPLCAWAVFPPESVAFRTAVLSVTYACPDGLHCGEWDYIDYIFLRRVGGQAAPA
                     QDLEVARMISPYGWRFDSTWSFTWHVDITDFAWMLHDSVEVEFLHTGYESNTDRGWVI
                     TLDFALTEGRPAMTCLGMETLWQGSIPYGDSARPIARTLAPVSVIAPTGAARARLRIL
                     QTGHGMDDQENCAEFCRKTRRIIVNEVLVDERPVWRTCGDNPLFPQAGTWLFDRANWC
                     PGSIVQPDLYDLPVTPGSTHTVALEMEPYVNPGNPSAAYVLSSYLFYYAPPWAAHDAT
                     LEEILIPSSADEYSRLNPSCRGARLLIRNSGREALTAVAISYGPPEAPQTFEWTGELA
                     SQARTEVALPGSIAGDRPFAARLELPNGRPDEYPADNELTSTGPAVPVYAPDLVLALR
                     SNNDSAHNSYRLTDGSGAIVRERALGTLGAGALYRDTLHLAPGCYELTVDDTAGDGLD
                     FWFNPEGGYGYVRLLDGEGRLVKSFVPDFGRGIRHSFMVAQDAPPAAAAEPLPIVNPF
                     PIRNRGVFAIDFFDNDPHPVAVTVLSEDSSRTVYERSYPEVREMMIPVDISAEPDGYY
                     WVRVTAGDRTATRRIKVTHQD"
     gene            29017..>31618
                     /locus_tag="PKM94_05375"
     CDS             29017..>31618
                     /locus_tag="PKM94_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017597624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD66106.1"
                     /translation="MKNQDVKVCAAMSGGRWPAVSAILFLLAAAPALAESPESVAGGP
                     PRTDSPALIGDPQSAPAENVYYINFDSNLEGWYFLNHPDTVQEYYPDQYTCDELTRMR
                     ANGASFALSWGYFDGSGAMMVTCPFRGWYRDSCYYSWRIKSVQVNVPDEWDGLYRVVA
                     RVYKGTGSRIWAKIGYRPAGSANTYYLPGWSELPTEGWTTLTLSEPSQGAFADLDAVS
                     ILFGAYSSDGNVYVDWVQAYEDQPDVPVLVAPANDQTIQANLPTFQWTGNGDYYTLQV
                     ATDPKFTNVLIDTSYIYSTSCKVTRPLPFSDYDYLPGGGGAHDPHYYWRVKAHRVGPS
                     GVSLYSNYFQFIINGPHEVPSEYPSILAARGALPPYIGGTVLVAPGTYTGRENCSIGL
                     GGEKPVDIIATGGAAVTVIDCQNTYYAFSYDAYTAGAPVVEGFTIRNARADGSDEAAI
                     VCRTGAGTIRNCIIENGAGTGVLARSGGIAYLVNTEIRNQTGDATEATDGGRILASSC
                     RFIGNDGYGGRLIQSSAGAGACSYDSCLFADNGSEGGGGLYVGAATSLGITNSTFTGN
                     VNGIEFATTGQSVTTIANCLIAFSNGRGIDWTSYSHDLSVLCSDAYGNGGGDWVNLAA
                     NELGLRGNIQRDPLFCDATAGDYRLAAGSPCAAEYSACGGIGLFGIGCILELGCAMAA
                     SDSGGPAPWTISFSAWASRGQPPYSYHWDFGDGGSGSSDTALHTYTVSGVYTVRLTVT
                     DQLGATCEQTSAVQIGPGLSCTAAADPTSGPAPLVVSLQAAGSGGVPPYRFSWAFGDG
                     GGSTQQNPIHEYSAEGVYTCSLAVFDQRGYSCTSGLSIVVGEPLQCAAAANPARGPAP
                     LTVNFSASASG"
BASE COUNT         5464 a        10004 c        10559 g         5591 t
ORIGIN      
        1 atcattctcg gggcggcggt gatcgacgac gtcatggggc tggtgatcct cgcggtggtg
       61 acgggcgtga tcgcggccgt ggcccacggc ggcggcgaag ggattacctc gctggggatt
      121 ctgtggatac tcggcaaggc ggtgctgttc atcgccgggg ccatcgtcat aggaaactac
      181 atcctgccga gcgttttccg ggtcgctttc cgcctgcgcg ggcacggcat gctgctgtcg
      241 gtgtcgctcc tggtcctgtt cgcgctgtcg tacgcggcgg gaaaggcggg cctcgcaccg
      301 atcgtggggg cgttcgcggc ggggctgatc cttgaagaag tgcattttcg ccgcttcacc
      361 gagcgcgggg aacactcgct ccgcgagctg atcgagccga tcgcgacctt cctcgtgccg
      421 atcttcttcg tgcgcatggg catgatggtg gatctcacgg ccttcggccg ggtcgagatt
      481 ctagggtttg ccgcgatgct cacgctggcc gcgattgtcg gtaaacaggc ttgctcgctg
      541 gccattttcg accgccgcac caaccggctc gcgatcggac tgggaatgat cccgcgcggc
      601 gaggtcggac tgatcttcgc cgggatcggg ctcacgctca tgctcgacgg gcacgcggtc
      661 gtgtcgagcg aaacttacgc tgcggtcgtg atcatggtca tcgtgacgac cctggtgacg
      721 ccgcccctct tgatctggtc gatgcaccgg ggagggacca cccggaccga ggcgccggcg
      781 gaaaagtccg gtgcggccgg ccggccgacg ccgtagaatg gagatcgcgc cgctgacaat
      841 cgcagggctg gagttccggc tgcgggaaga accgggaatc gaacgggaat ggttcacggc
      901 gcgcctgcgg gactatctcg cctatgaacc gggagggtgt ttcgcgctcg ccgaaggggg
      961 cgagacggtc gggatggtga ccgctttgtg ctaccagacg accgcctggc tggggtggct
     1021 ctatgtggcg gcggcgcgcc gcggcgccgg actcggggaa cgcctcatgc gccagggaat
     1081 ggggtactgc atcgcgcgcg ggatgcgctc gatcgtgctc gaagcggtgg tggaggcggc
     1141 gccgctctac cggcggatcg ggtttcaaag acagtttcgc acccagcact accgcctcga
     1201 aaaaactcgc cgcggcgcgg cgccctcgcc gagcgtggcc gtgcggcgct tcgcggccga
     1261 tgatctcgtc cgcatcgccg atctggaccg gcggatgttc ggacaggacc gccgccgggt
     1321 gctcgacctc gtgcggcgca atcccctgca ccgggggtgg ctggcggaga aagaggggaa
     1381 ggtcgcgggg tacttgtggg ccaccaaggc ggcggccaat aggcaggcgg gaccctgggt
     1441 gatcgacccg gggacggggg atgcggcgga aacggcggcc gcgttgtgcg acgccgcctt
     1501 cgcagagagc gaccggccgc taatcctgcg ctgcccgctc gtgaccgaat ctcgggcgcg
     1561 gctgtgggac gatctcgggg cgcaggcgat tgactatcac accgagcgga tgtatgcggg
     1621 cgaggagtat gcgcccgagg gagaaggtgt cctttgcctg ggctgtccgg ggaaaggata
     1681 ggcggcgggc cacgcaactt ggacgggcgc accggggcgg ggcggcgcgc gttcgggagg
     1741 agacatcatt tctcaagaaa tcgacgagcg cacgagaatc ggacttgaca acgggggttg
     1801 gcggggtatc ttggggtctg ttgatcgcgg ggtggagcag tctggtagct cgtcgggctc
     1861 ataacccgaa ggtcggaggt tcaaatcctc cccccgccat tactcaagtt ggatgctccc
     1921 ccgataatcc ccagaatccg ctccactcgc ggcttcccct ccagcatcac tccgcgctga
     1981 tcgttctcgg ttaccgctag agcggtcagg aggacccgca gtccggcgat atagcatgca
     2041 gcctcgtaca ctcgccgcga atcggcgggc ggatgtcgcc tcgatcagat cactgcgccg
     2101 acatgagctc gcattgggac tgcagattgt caccgaacat ctatgcccga tatgcaacga
     2161 aaatagtgat gtttgtccac aacatacttg caggccggtg gcatgctgac tataatatca
     2221 tcaaaggcaa tggtcattat cgtgagagtc ggctctccga ggaaacgcag atgcgtgacc
     2281 gaacacatcg ggccttcgtc gaagtcattc gggcgctgag cctgccgacg atatgtcacg
     2341 acgcgctgct ttggtctccc gatggtgatc ccgttccgcc cgggcccggg cgggcagggg
     2401 gcggcgcgcc attcggcgga attgagttgt ttcccgccgg aacattcggg accgatcgcc
     2461 cgtgcgccgg cgccgcatgg tatcgcgccg ccggcggccg cagcctttcg ttcaccgcat
     2521 ccggcttgcg ttcgctggcc ttaccaccac tgttttttct ctccattcgc tttgcccagg
     2581 aggtgacatc gtgatgactc gcgcgaggaa gcactatctc ttgttgatcc ctgcgatcgc
     2641 tttcgcagcg gccctcggcg ccggtggctg cggcacgaaa gaaggggttc ctgccgccgg
     2701 cccgccggag gtcagggtgg cagtggccgc gaccaaagat gtgcccgtca gccgcgagtg
     2761 gctcggccag accctcggag ctgtcgacat cgaaatccgg gcgcgcgtcg acgggtggct
     2821 ggagagcatt cacttccgcg aaggcagcga ggtcaggaag ggggacctcc tctacacgat
     2881 cgacccggcc gaactggagc agaaggtggt tcaggcgaaa gccaaggtgg ccgaggcgca
     2941 gacgatgttc gtgcgggcgg aatccgacgc caaccgctac cgtcctcttg cggaggccaa
     3001 agcggtgagc cagcgggacc tggaggcggt tctggccgaa tacggtgcac ggcaagggga
     3061 ggttgaggcg gcccgggcgg ctctccgcct ggccgagatc gagctcgggt atgccacgat
     3121 cacggcgcct atcgacggcc tgatcggcct gtcggccgcg cgggtcggcg acctggtcgg
     3181 ccgtccgccc agccccgtca tcctcaacac cgtttcccgc gtggattcgg tccgcgtccg
     3241 cttctccatt acggaaaacg agtatctgga gttggctcgg cggctgcagg ccacgcccga
     3301 cggtccgccg gcgcgcgagc gtttccccct gcgcctgatc ctgtctgacg gcacccgcta
     3361 cccccatgtg gggagcgtca gcgtcgggca gcgcgagatc gatccggcca ccgggacgct
     3421 gcagcttgag gcgctctatc ccaacccgga gcgcctcctg cggcccggcc agtttgcgcg
     3481 ggtggtcgcc gaggtcggga tgcgcaagga cgctgtcgtg gtgccggcgc gggccgtgac
     3541 cgaactccag gggcagcaaa tgttgttcac ggtcggcgca gacaacaagg tggcggtgcg
     3601 ccgggtcgcg gtcgggccct cggccggccc cttcaccgtg atcgaccgcg ggctggcggc
     3661 gggcgagaaa gtcatcgtcg agggccagca gcgcgtccgt cccgatatgc cggtccggcc
     3721 ggtcgagata ccggcggatt ccctcgcggg cggcggaggg cgctgaccta tgtcgaaatt
     3781 cttcgtcaac cgcccgatcg tcgcgatggt catcgccatt gtcatggtcc tcgtcggctt
     3841 gatcgtcatg cgcggcctcc cgatctcgca gtttcccgac atcgtcccgc ccatgatcaa
     3901 catcgccacc acttacgtgg gcgcctcggc tatcgatgtc gagcagtcgg tggccacgcc
     3961 gctcgagctg caggtgaacg gcgtggagga catgatctac atgaagtcta tcaacacgag
     4021 cgacggcatg atgacgctgc aggtgtcgtt cgaggtcggc gccgacctgg acgtcgccaa
     4081 catgctcacc cagaaccggg tgtcgcaggc ccaggcgtcg ctacccccgg ccgtgaagaa
     4141 ctatggcgtc acgaccaaga agtcgctcgt gttccccctg ctcctggtgg ccctgacctc
     4201 gtccgaggag tcctacgaca atatcttctt gagcaactat gcggctatca acatcaacga
     4261 ccggctgagc cgcatcaacg gtgtcggcga cgtcaagctt ttcggcggcg gcgactacgc
     4321 gatgcgcatt tgggtccggc ccgatgccct ggcgcggctc gggctgaccg tgtcggacct
     4381 cgtgcgggcc gttcaggaac agaacgtcat cagcccggcc ggccagctcg gcgctccccc
     4441 ggcgccgccg gggacggagt tcacctacac cgtgcgcacc cagggccggc tgttcaacga
     4501 agaggagttc ggccgcatcg tgctccgcac caatcccgac ggttctcagg tgcgcctgcg
     4561 cgacgtcgcc cggatcgaac tggggactct catgtacggc cagacgagcc gcaacaacgg
     4621 caagccggcc gccgtcatcg gcgtgtacca gctccccggt tccaacgccc tcgcggtcga
     4681 ggagagcgtg agcaagacga tggaggagct cgcgcagcgc tttccgcccg acctcgtgca
     4741 cgagattacg ctcaacacca cgcgggcagt ggaggagggg atcaacgaga tcgtccacac
     4801 gctgtttgag gcggtgctgc tcgtgatcct ggtggtgttc ctcttcctgc agaactggcg
     4861 ggccacgctc attccgctga tcgccgtgcc ggtgtccctg gtggcgacct tcatggtgtt
     4921 tcccctcctc gggttctcca tcaacacgct gtcgctgctg gggctggtgc tcgcgatcgg
     4981 catcgtggtc gacgacgcta tcgtggtcgt cgaggccgtg atgcaccaca tcgaggaggg
     5041 tttgccgccg aaggaggcga cgctgaaggc gatggaggag gtgtcgggac cggttgttgc
     5101 tatcgccctc atcctgaccg cggtgttcat tccggtggcc ctcatcggcg ggatcaccgg
     5161 ccagctgtac cagcagttcg ccatcacgat cgcgatctcg gtgctcttct cggctttcaa
     5221 cgctctgacc ctgagtccgg ctctggcggc gaagctgctg cggcccaagg gccaggggcg
     5281 gcggtcgccg ctgcagcgtt tctacgactg gttcaaccgc ggtttcggcc ggttcaccgg
     5341 cggctacctc tccgtcaccg gcatcctcgt gcgcaaggtg atgcgcggcg tggtcttcat
     5401 cggcatcctg ctggcggcca cggcgctcct cctgaagggc gttcccaccg gtttcgtgcc
     5461 cgaggaggac aacggctact tcctcatcac cgtccagctc cccgacgccg cgtcgctgga
     5521 gcgcaccgac gccgtctgtc ggaagatcga ggcgatcctc ggagagacgc ggggggtcgc
     5581 caactacacg gcggtggccg gttacagcat cagttcccag gcgcccggtt ccaacgtggg
     5641 cacgtttttc gtttcgcttg agccctggga ggagagaacg accggggagg agagttcgtt
     5701 tggtctggtg gcggcgctca accagcgctt tgcccgcgag atccccgggg cggcggtgtt
     5761 cgcgttcggg ccgccggcca tccagggcct cggcaccggc gccgggttca ccttcatgct
     5821 gcaggacatg accggcgggt cgccgcagga actggccgcc atggccgaac aatttgcggc
     5881 ggcggcgcgc cagcgccccg aaatcggccg cgtggcgacc ttctaccgcg ccggcgtccc
     5941 gcagatgtac gccgaggtgg atcgcgacaa ggcgctcaaa ctgggcgtga gcatcgccga
     6001 cgtcaacacc accctgggat cgctcctcgg ggcaaactac atcaacgact tcaaccggtt
     6061 cggccgggtc tataaggtgt acctggccgc cgagggcgag taccgcaacg agacgtggga
     6121 tctcggactt ttctatgtcc gcaacaagaa cggcgagatg gtgccgctga gcaccctggt
     6181 gaccatgaaa ccggtttcgg gtccccagta caccaaccgc ttcaatctgt accgatcggc
     6241 ggagatgatg ggcggtccgg cggccggcta cagttcgagc caggcactgg ccgcgctcga
     6301 ggaggtggcg gcggacgtcc tccccgccgg ctggggttac gactgggcga acatgtccta
     6361 ccaggagaag aaggcggccg gcacgggcgg agttgtcttc gtcatggccc tcgtcttcgt
     6421 cttcctgatc ctggccgccc agtacgagag ctggtcgctg ccgctgagcg tgctcctcgc
     6481 cacgcccttc gccatcttcg gcgccatgac cgggctctgg gtgtcgcgga tggtcgatac
     6541 cacgtacgtc aacaacgtct tcgcgcagat cggttttgtg acgctgatcg gtttggcggc
     6601 gaagaactcc attctgatcg tggagtttgc gaagatgcag aaggagaagg gtctgccgac
     6661 cgtcgaggcg gccctggcgg ccgccaaact ccgcctgcgc cccattctga tgacctcctt
     6721 cgccttcatt ctcggcgtcg taccgctcgt gctcgccgcg ggggcgggcg ccgaggcgcg
     6781 caaggtgatg gggatggcgg tcttcgccgg cctgctcgtc gccaccagcc tggggatatt
     6841 cctgatcccg atgctgtacg tcgtggtgca aaaatacttc gtgcgcgaga ggtcccccga
     6901 gcccgcccgg cgttgcgacg acggcgccgg ggccgcagag aaaggagcag gcgtatgatg
     6961 aaattcctca tggccgggtt ctcggcggcc ctcgtggccg gatgcgcggt cggccccaat
     7021 tatgtccggc cggagatcga gacacccggg cggtggcaga atcccgactc cgttttcgcg
     7081 gccgacaccg ccgcggtgcg ctctgagacc gcctggtggg gcctgttcgg cgacaccgtt
     7141 ctgaccaatc tcatcgccgc cgccctgcag gaaaacgccg atgtccggat cgcggccgcc
     7201 cgggtggagg aagtcatggg ccggtacggc gtggcccggt cggatttctt tcccaagctc
     7261 ggagccgccg gcgcggcctc gcgcgggcag ctcttgtcgg acgaggccgg ccgcggtctc
     7321 gagtccaccc gcaaccgctt tcaggtgaat ctcgcggccg actgggagat tgatttgtgg
     7381 ggcaagctcc gccgggccac cgaggcggcg cgggccgact tgctggcgac cgaagaggcg
     7441 cgccgcggcg tggtgctgac cacggtcgcc ctcgtcgcga gcacctatgt cgacctgctc
     7501 tcgatggatt ggcaacttga gattgcgcgc cagacggccc tcacccggcg ggaggcgctc
     7561 gatctgttcg aggtgcggcg cgaaaagggg gatctgtcgg atctcatctt cagccaggcc
     7621 gaggcggagt actggcttgc catgtcgcag atcccgctca tcgaggagaa catcgccttc
     7681 ctggaaaaca tgatcagttg cctggtcggc cgcaaccccg gtccggtggc gcgcgggaag
     7741 acgctcgagg atctcgcgcc gcccgccgtg cccgtcgggt tgccgtccga actcctcgag
     7801 cgccggcccg acatccgctt cgccgaggag cagctgcgcg ccgccaacgc ccggatcggc
     7861 gtggcccggg cgctctactt cccctccctt tcgctgtcgg ggctgctcgg ttacgcgagc
     7921 gacgatctct ccgatttggt caattccgac acgcgcacgt ggcgggtcgg gggcgatgtt
     7981 ctcgcgccga tatttcgatt cggcgagatc aaggggcagg tcaaggcggc cgagggccgg
     8041 cagaaacagg cgctctatgc ctacgtcgag gccgtgcaca acgctttccg cgacaccgaa
     8101 aacgctctcg tgcggcgccg ctgcacgaac gagcagctcc aggcggaggg taaacgcgtc
     8161 gccgccctcg ccacctacgc gcgcctcgcc cgcatgcgct ttgacgaggg cgtgaccagc
     8221 tatctcgagg tcctcgatgc cgaacgggcg ctcttcgcta ccgtgctcgc ctacacccaa
     8281 accacagcgg acctccacaa gagcgtcatc gctctccacc aggcgctggc cggcagctgg
     8341 ctggacgccg cggccgccga atcatttcgg atcgaggagg gcatcaagct ccgcgggcaa
     8401 ccgtgaacca aagcttctgc gatacatcgg aagccaagac cggaagtcac gattctctgg
     8461 gacgccgggt accacaactt ttcagcgaat ggaggctcac accatggaca gcaatcgtac
     8521 tacctgcatg ctagcggccg tcatgcgcgc ggtcatcgta gcgggcgcgg ccctgctgct
     8581 gttgggcggc tcgccggcgc acgccgagca ttggttcttc ggcgtcggca ccggcttcac
     8641 gttcatgaat atccaggggg aacagggcta caccgtcatc aactacggac cggttcagta
     8701 cgacctcgac ctcgatccgg atgacatcag cgatgtcttg gaatctgcgt tcggctttgc
     8761 cgggtacgca actgacggta cgtggataat ccagtactcc ctcggtgtgt tcacactggg
     8821 cggagacggc agagtagatc tcccgcccga ggtcgggggc ggaaccctgg atgttgatgt
     8881 ggagttcaac gtcacgaacg gcaagattac gcttggccgc accgtctacc gcgacaaaaa
     8941 gatgaaattc agtttcaccc cgtacgccgg actacgctac ctgaggcatg aaatcaacgc
     9001 cgacttcgtg gtgactcagg gagacgagtc tacaccgatc ttccggggtc gggattataa
     9061 gtggaccgac gcgctcgtcg gtgcaacaat cagctaccgg ctgacgccca gattcacctg
     9121 gactgcgagc ggcgacgccg tcttcggagg ttcggacgga accttctact ttacgacgag
     9181 cctgcctttc accccgacgc cgcatttttc gtttggcccg aatctctcat ataccgcgat
     9241 tgactatgaa aatgacgata agggtgatgc agactggtac ttgtacgatg ccgacgagtt
     9301 tggcgcgggc atctttctag ccgtcaattt ctgacgccgg actttgagcg gaccggcaga
     9361 gaaaacgagg cggattggcg ctggccgagg agagttgcga cagcattctg agaggtggtt
     9421 gtgcccgacg ctgacaagaa gcacccgtca cttcccgacg gattcaacgt agccgacccc
     9481 gctgaggctc cctggctgct ggacagatac gatgcgccgc acctggcggt gagtcgcggc
     9541 agccggcggc ggttccattg catgatcaag gcaatcggat cggcctgcaa tctcggctgc
     9601 gcctactgtt tctatctgag caaggcgcat ttgcccgggg ggcccggaag ggggcggatg
     9661 tcccaggaga tgctggcacg gctcattcag cagtacatcg aaggcgtgac cggcgatgaa
     9721 gttgtctttt cctggcaagg cggcgagccg acgctgctcg gcctggactt cttccgccgg
     9781 gtcgtcgccc tggaaaagca attcgcccgg cccggtcagc gtatcgagaa tgatcttcag
     9841 accaacggca cgttgatcac ggaggagtgg tgcgaatttc tcaaggagaa tcggtttctg
     9901 gttgggctga gcatagacgg cccgcgggag ctccacgaca cgtaccgggt cactagaaca
     9961 ggccgaccga ccttcgacag agtgtttcga gccgcgatgc ttctcaggcg ctacggcgtt
    10021 ccgtacaaca cgctgacgtg catcaatcgg atgaacgccc ggcgcccgct ggacgtttat
    10081 cgcttcctgc ggcatgaggt cggatccacc tatatgcagt tcattcccgt cgtcgagtac
    10141 aggggatttg agctgaccgc cccgcacagg tggcacaacg acgcgctgcc gagagatggt
    10201 gaggcagcag ctcgaccggg gcatccggat tcgattgtaa ccgactggtc ggtcgatccc
    10261 gaggactggg gttacttcct gtgccggacc ctggatgagt ggctgaaccg cgacttcggg
    10321 aaggtgctgg tcaaccactt cgaaacgctc gtgtcgcagc gcctgggccg gggctcacaa
    10381 ctgtgccttt acggcgagtt ttgcggcaag ggcgtggcgg tggagcacga cggggcggtg
    10441 ttcgcctgcg atcactacgt ctacccggag taccggctgg gcagcatcgg cgaaacatcg
    10501 ctcgtcgacc tggtgttctc gcgcgcacag gcgcggtttg gctatgccaa gagcgaaacc
    10561 ctgccgccgt ggtgccggga ttgtccttac ttgaccgatt gctggggcga gtgcccgaag
    10621 aaccggatca ttcgaacggc cgggggggaa ccaggtctga attatctctg ccgcgggttc
    10681 aagaagttct tcgcccgcgc catacctgag gttgagcgcc ttgccgccgc attgcgccgg
    10741 tctccgcaac aaccacgcaa gcgaatgtaa gacgtaagga agtgcctgta tcggaatgat
    10801 agcggccatt agattgaacg gcgaatcggt cgcaggtacg ctcgcgaccg gtttcctcgc
    10861 ccctgccgca acagacttga gcggcgacga cccacttcct tcgcggaacg aaggtcccgc
    10921 catacaggcc accgccgctt tcgtcagcaa gtcggagggt gatcggtaga tgctggagtg
    10981 gacgcaggcc cgcggaggtt cgcggttgat gctgctggcg catcacgacg atgccgcgcg
    11041 cgagtgccgt tacaggactg aatcggaggt cagcatgttc agcgatgagc tccggactca
    11101 ggcggaacac aacggctgga cgattgtcag tatgaaggac ggccggaagc gcgttctccg
    11161 gttcaagccc accagccggc gcgtcaagga gcgatccggt cggctcgatc ggtccgcaga
    11221 taggaaagga atgtgcatgg agacaatgtg ttgagcaatg acagagcgac gcaacagcaa
    11281 acaaggaaaa gccgcggcca tcaggcccgg ttcgcaggag gtaacaatgg gaacaccgca
    11341 cagacccccg gagaagacgg ttaccggcga attcaacagc tgcgggcgaa ctgtggccat
    11401 gctgggtatc gcgggcgggg tggcgttcgc aaccggcacc atgccggcac aggcccagac
    11461 cgcgcccgca cagggccaga agcccaacat tctcgtcatc ttcggcgacg acatcggctg
    11521 gtctaacatc agcgcctaca atatgggcat gatgggcagc cgcacgccga atatcgaccg
    11581 gctcgcaaaa gaaggggcga tcttcacgga ctactatggc cagcagagca gcaccgccgg
    11641 cagggccgcg ttcatcaccg gccaacaccc cttccgcgtc ggattgctga aggtcggtat
    11701 gccgggtgcg gagctcggac tgcaacctga ggatcccacg atcgccgatc tgctcaaaaa
    11761 cgaaggctac gtcaccggcc aattcggcaa gaaccacctc ggcgaccgca acaagttcct
    11821 gccgacggtg cacggattcg acgagttttt cggcaacctc tatcacctta atgcggagga
    11881 ggagccggaa gacccgcagt atcccaagat gccggagttc atccagcagt tcggcccgcg
    11941 cggggcgttg ctgtgcaagg ccacggaggt cgacgattcg accgtggacc cacgctttgg
    12001 ccgggtcggc aaacagacga tcaaggacac cggcccgatt actcgcaagc gtatggagac
    12061 cgcggagaac gagttcctca atgcgtcact agacttcatg gaccgcgccg tcaaagccaa
    12121 caaaccgttt ttcatctggc ataactcgac cagaatgcat gtctggaccc gactatcccc
    12181 caaataccag gacaagacgg gatatggctt gtttgccgac ggcatgcagg aactcgacga
    12241 cgttgtaggc gccctgttga agaagctcga cgacctcggc atcgccgaca acacaatcgt
    12301 ggtcttcacg accgacaacg gcgctgaaaa ggccacctgg ccggatggcg gggccacgcc
    12361 gttccgggga gagaagggca gtacgtggga gggcggcatg cgcgtgccgt gcgctatccg
    12421 ctggccgggt gtggtgaaac ccggcacggt catcaacggt atcatgtcgc acgaagactg
    12481 gacgccgacg ttcctggcgg ctgccggtga gcccgacgtc aaggacaaac tcctgaaggg
    12541 gcacactgcc aacggcaaga cgttcaaagt tcacctcgac ggctacgatc agcgggagat
    12601 gctggccggc accgggccga gcaagcggca agagatcttc tactttgacg acaacgggaa
    12661 cctcaacgcc gtgcgggtgc gggactggaa ggcccacttg gctctgcctg gtgactggta
    12721 tcaggccggc gacccggtgc cccagacctt tcccaagatt gtaaacctgc gcatggatcc
    12781 gttcgaggac catgtccttt ttggcgagtc gcccatggcc gcgcggtgga tgaccgacaa
    12841 gttctgggcc ttcgtgccga tgcagggtgt ggtggcgcag tttctgcaga cattcaagga
    12901 gtttccacca cggcaaagat cggccagctt caacatcgag caggtcatgc tgcaactgca
    12961 gcagagttca acggcacgca attaacgttg tgagtccggc cacaggcgcc gcaagataag
    13021 gcatacggtt tgacggcggc caggatgctt tccggccgcc gtcgtcattc ggtgttccct
    13081 ccgcccgtca aacgggcgac cggcggcctc agggcgcgag gagcttgctt gagatcaatt
    13141 cccggatgat gccgccggcg agacccttcg actcctcttc gaggtttgcc ggattggttg
    13201 tccggctggt gccggtccag acgatgttga acccggacgt ctcgtagacg ttggtctcga
    13261 tgctcacgac gtcggatgcc gtgacgtaac cgggatcatg atagaaagcc caggcgtagt
    13321 catagaaccc gtagtagccc cggtagtgcg cgggccaggg gacggccagg gcggtcgcgg
    13381 gcgtgatctc gatccgctgg gtgcggtcga ccacgcgcgc cgtgatgatc gcgtcgatcc
    13441 cgttttccct gacgagccgt tcgatttctt cgcggggcat cttctcgtcc gtgcggtaga
    13501 cgtcgtagct ggggacggcc gcgaccccct gttcgcgcag ggtggcggcg aaagcggttt
    13561 caaaaatgcg ccgggaggat tcccggtcgg tgatgccgac gaccgcgatc ttcttgaacg
    13621 ccgggccggt gtaggccgga tcgcgccagg tgtcgacgac gcgcgggccg acggcacagc
    13681 cggcgacaag cgccgccgac aggatggccg caagtgcatg gcgctgcctc ataccaagac
    13741 ctccttggga ttcgccacat ggttccgtat ctcaggaaga aaccgaccgg gcggttcacc
    13801 cgcaagcgct ttcccaaacc ggcgggccgc ccttcggcgc gcagcggcgg ccgcgggtgc
    13861 ggaaagagcc gcgccgctgt tgacattcgg gagggcgcat agtatcgtat cttctgacgg
    13921 caggtagtcg gactcgtgcc gccgcccgcc cgcagatcgc cggcggcggc cctgacatga
    13981 ggttcccttc attgttcaaa accgaacagc aggacaggat ccgctctcac cggcggacgg
    14041 ttccctgttg ccgcaccggg gagtctgtgc tatgccctgg aacagcccgg ccaatcaaag
    14101 gcgcctgagg cgcctgtttg acgaggggtt ctcggcgatg gacatcgccg agccgctcgc
    14161 gtcgttcgac gacgaccgtt cggcggcggc ggtccgggct ttcctggagc agagggatct
    14221 catggtcgcg ggtgtgcgcc gcgacggctt cgtgtgcggt tttgccgaca ggcgagaact
    14281 cggccgcggc cgcctgggcg cttaccttcg cccgttccct cccgagtgta tcctctccga
    14341 ggccgattcc cttcgcaaag tgatactggc gatgcggggg ggaggcccct gtttcgtcag
    14401 cgtactgggt gaagtcggcg ccgtcatcac gctggccgat ctcgagaagc ccccggtccg
    14461 gatgttcctc ttcgggatga tcactatttt cgagatgatc atgacgtggg gcattcggac
    14521 atatttcccc ggcgattcct ggcgcgccca catcccgccc ggacggctgg ccaaagccga
    14581 gcaactgtgg caggagagac agcgccgccg gagcaccgcc gatctgctgg attgcctcca
    14641 gttctccgat cgggcgcaac tgctgctgag gatcccgcgc gtgacggacc tcctggccca
    14701 gggagggatt ccatcccggc gggcggcgct caccgcgatt ggcgaactgg aaacgctccg
    14761 caacaatatc gcccactccc aggagatcat ccccgagagc tggtcccgca tcgtcgtctt
    14821 ttccacgcgc ctggaactgc tcctcggcga ggctttccgg agttagcgcc gagaggagga
    14881 atactgactg tgagaagctg caaactggcg ggtgcggcgc ttctgcttgc cgtcctcgcc
    14941 tggccgctgg cggctcagga tgcgccgccg gtcgacgggg gctggccccg gaccttcact
    15001 accgcaagcg acggcgaaat gacgctcttc gccccgcagg tcgccgcctg ggagaatcag
    15061 gagcggctcg tcgcattctg cgccgtgtcc tacctcccgg ccggggcgac cgagcgccgc
    15121 ctgggcacga tcaagatcca tgcggccacc gaggtctcgc tcgaggagcg gctcgttcgc
    15181 atgaccaacc tccagatcgt cgagtcgaac ttccccgggc tccccaaaga ccaggtgcgc
    15241 gggctgctcg ccgaactgat ggcctcgtac gccgacacgg cgagggtgat cgcgctggat
    15301 cggctgctcg cggcgatcga caagagccag ctcgggccgg ggaacctcga cggggtgaag
    15361 gccgagccgc cgcccctcta tgtcagcacc cgcccggcgg tattggtcat tttcgacggc
    15421 gcacccgtct ggagtcctat caaggacaat gacctgaagt ttgcggtcaa caccaactgg
    15481 gactgcttcg cacacgctcc cacccagacg ttctacctga ggtgggagga ctcgtggctg
    15541 caggcgcccg acgccggcgg cccgtggtcg ccggccggca cgctgccggg gagttttgac
    15601 aaattgcccg acgacgggaa ctggaacgaa gtcgccgcgc acctcccggg acgcgcgctg
    15661 ccggccgccg aggcgccggc ggtgtttgta tcgacggtac ccgccgagct gctgctcctg
    15721 gagggcgagc cggaatacga ggcggtcgcc ggaacgcggc tgttctgggt ccgcaacacc
    15781 gagagcgatg tgttccgcct gggaacagac ggaaccgtgt acctcctgct ctccggccgg
    15841 tggttctcgg cccctgattt caccggaccg tggacattcg cgacgcccga tctcccggag
    15901 gacttcagga acatcccgcg cgagcacgag cgctccgacg tcctggcgtg cgtgccgggc
    15961 acggatgagg cggcggaggc cgtcctgctg gccgggattc cgcagaccgc gcgggtgaac
    16021 aaggcggaac tgaagccgcc cgaggttgtc tatgcgggcg atcccgaatt cacgcccatc
    16081 gagggaacct cctgccgcca ggccacaaat acggacaagg acattctcag agtcggcgac
    16141 ctgtactaca tgtgtttcca gggggtctgg ttcatgtcgc ggacgccgac cggaccgtgg
    16201 gaggtcacgg gtgacgtgcc ggccgccgtt tacgagatcc cgcccagttc cgagtgccac
    16261 cacgtgacct acgtcgtcgt cgagcactac gatccggcgg atgtctacgt caccttttcc
    16321 tacacctccg gctataccgg cgtcattatc gcctgggggg tgccggtcta cggcacggga
    16381 tggtactacc cgccgttcgt gtactggggc tatccctatc cggtgtacta cccgcgctgg
    16441 gtcacctacg gatatgccgt gcggtacaac ccctggaccg gcatctacgc cgcctcggca
    16501 cggatctacg ggccgtacgg cggggcccgg tatggcgtgg cgtacaatcc gcggacggga
    16561 acgtgcgtcc gcggcgcgat ggcctacggc ccgtacggcg cggcgggagc ggccgcagcc
    16621 tggaatccgc gcaccggcgc gtacgcggcc acgcggcagg gcgccaacgc gtacggcagt
    16681 tggggcgaat cgtacgtcca gcgcggcgac aactgggccc acaccgcgcg atacacggac
    16741 gcggccgccg ggcagacgaa acgggtcacg cgaaccgatg aggcgacggt cgtgaacacg
    16801 cgcggtccgc agggaggcgg gttcatcggc gccaagggtg acgacgtcta tgccggccac
    16861 gacggcagcg tgtacaagcg cgacgagaac ggtaactgga gcaagtacag cgacgggggg
    16921 tggacggata cggagaagcc ggcccgcgcc ggcgagcggg ccaaagggga ggccgaccgt
    16981 ccttcccgag acgccgtcgc gccgcaggcg ggcaaagcga gcagcgggac accgtccgcg
    17041 gcgtcaaccc tcggccagct cgagcgggat agcgccgcgc ggtcgcaggg cgcgcgacgc
    17101 gcggatcagt acggccggca gcggaagcag gctgcgacga gcagccgcga agcggcccgg
    17161 ccgcgcagcg gttccggggg caggcggcgc tgaaggcttc gatgtgttat gtcgccacgg
    17221 gtggcggacg ggatgtcccg ccccgggaaa aacaacgctt cgacatccgg cagcccgcgg
    17281 tcgggctgcc ggaattttca ccgcaggact agaaccggac cacaagtccg agcgagagct
    17341 ctccctggaa gagccagttg tcgtcggtga ttgaccacac cccgccccac gggtctctcc
    17401 aataggcctc ctcggccggc aggctggtca tgtaaccccg gaaacccagg cgcgcgccga
    17461 ggcgctcgct gaacatccgc gccgccccgg cgccgaacga gaacgagaat ttcgtccggc
    17521 tgtccgctcc gccgtcgggc gcggcgaaaa atgttgcgcc gacgccgaag ttggcgaaag
    17581 gcagccaacc ggagtagttg ccgaaccgat acaggccgcc gccgtggaga tacatcacct
    17641 cgatgtcccc caggatgagc ttgtcatcct ttacctcgaa gcggtcgaga tacacatccg
    17701 ggactttgtc catcgacgcc ggttcgaacc ggagatccga cctctggtag ctgaaactga
    17761 gttcgatcat cgcctgttgc ccggtggggc cgatcccgcc gaccggaaag tcacacgtca
    17821 gaccgaagct tacgccgggc gccacgtcga gcctgtcggc gaggtcgttt tcgtagtaac
    17881 cctcgcccag cgcggttgcc gtgcgcagcc ccccgtacgg ctggagttcg acatccacct
    17941 tcgcctcggc ggccgcggcc gtcaagatga tgagcgcggc gagccgcgtt ccccttcgca
    18001 acatactgac ctcctgactt ccatgaagtc ctgcttcttt gtgcgttcgc aacctctccg
    18061 gcagccggtg cgggcgacag cccgggactg cccgcgatgc tgaagtgtcc aatgagaaca
    18121 ccaggaacct ttcagccgct gcgcacatct gcgagcgggc gccgcataag agccctcagg
    18181 ccaacggctc acccatcacg gtcactcaag taacccccgc tctcctcagg tgtcaagagc
    18241 caaacggcgc cgtcgctgcc ggatcggctg gaatggagat ctgtccacat gcgtgagtcg
    18301 cgggggaacg ccaaccgcaa gcctgcgttg ccctgtcatc ttctgcccgg ccggcgccgc
    18361 cgacctccat cgggctccct tcgcttcgag aatggtcgct tcgaggtcac accgcgctgc
    18421 gaccccctgt cctgccctgc cggcgagagc gccggcggct cccggccggc ggatgccata
    18481 gcacggccgc cgaaatcgcc gcttgccaga cgggtggaga tcagagtatg ctgtagacaa
    18541 tggattcttc acgcgcggtc aggcgcgaaa gcggaatgac gcggtcgcat tcaggcggag
    18601 cgggagccgg ttggtcttcg gcccgcccga tccggcaacg tcccgcggcg atgcggagga
    18661 caaggtgatg cgctgtattc ccggcgtgca tacgcggacg gcaatcggtc tcgtctgtgc
    18721 ggcgttcttc tggctggtcg gctcggttgg ttgggggcag gcgggtgggg aggccgaagc
    18781 ggaaaccgac ccgctggtgc gggcgaaact ggaatggttc caggacatga aattcggtct
    18841 gctcatgcac tggggcactt acagccaatg ggagatagtg gagagttgga gcctctgtcc
    18901 cgaggacgaa gactggtgcc gtcggcgcgg cgaacaagcg ggggattact tcgaatacaa
    18961 gcgggcctac gaggatctgc agacgacgtt caacccgacg aaattcaatc ccgactcctg
    19021 ggccgcggcg gcgaaagccg ccgggatgcg ctacgtgatt ctcacgacca aacatcacga
    19081 tggcttctgc atgttcgata ccagggagac cgactacaag gtgacgtcga gcgcctgtcc
    19141 attcagcgcc aacccgcgcg ccaatgttgc gaaggagatt tttgccgctt tccgcacgca
    19201 gggattcggc atcggcgcct acttctccaa gcccgactgg cacaatccga actactggtg
    19261 gccgtacttt ccgcccttcg accgcaacgt caattacgac attgcccgct accccgagcg
    19321 ctgggaggcg ttcaagacct ttaccgccaa ccaaatcagg gagctgatga ccgactatgg
    19381 tgcgatcgac attctctggc ttgacggcgg ctgggttcag ccgatgacgg ctgcctcgcc
    19441 gcgttggggg aagcacccct gcgaccagga catcgacatg ccgcgcatcg cggcgatggc
    19501 gcgctcgctg cagccggggc tcattgtcgt tgaccgggcg gtcgagggcc gctaccagaa
    19561 ctaccggaca cccgagcagg aggtccccga tacagcgctc gactatgtct gggagacgtg
    19621 catgacgatg gcgacgtcgt ggagttacgt ctcgaccgac acttacaagc cgacctcgca
    19681 gctaatccgc ctgctggcgg aggttgcggc caaagggggc aatctgctgt tgaacatcgg
    19741 gccgagtccg gagggggagc tgccgccgac ggcgctggcg cggctggccg aaatcggcgc
    19801 ttggatggcg gtcaacggcg aggcgattta cggcagccgg gcggttgcgc catatgggga
    19861 aggccgcgtc tgtttcaccc ggtcgcccga cggcagtatc aatgccatct atcttgccgg
    19921 cgacggcgag caccgccctc cggattcgat tgcgatcgcg tcctttgcgc ccaaagcggg
    19981 cagcgaagtc aggatgcggg gcgtcgagga tccggtggcg tgggagccgg cggcggcggg
    20041 attcgtgata cacgtgccgc catcggcccg agttcatccc ccgtgcgact acgcctggac
    20101 cttcaggttt gtaatcaaat gaccccatgc gaaacagtaa aaccctcgcc tgcggaacac
    20161 ccatgcgcat tctccaaatc atcgcggttg tgctgtcaac aatgctcgtc ccgctgagcg
    20221 gtcggggcaa gcaggccgac cagccgaagg acgagtcgaa aatgcagtgg ttcgccgatg
    20281 ccaagctggg gattttcatc cactggggga tctacgccgt ggacggcgtc ggcgagtcgt
    20341 ggtcctttca caaccgccag ttgccgtatg acgactacat gaggcagctg gcgggattca
    20401 cggcgtcgtc ctatgatccc ggggaatggg cggaggcaat cggggaggcg ggggcgcgct
    20461 atgcggtcct caccagcaag caccacgacg gggtggcatt gtgggacacg aagatgaacg
    20521 acctgagtgt gccgaggcgc acgccggccg gacgggacgt gattgcgccg tttgtcgagg
    20581 ccctgcgcgc ccgcgggatc aaagtcggac tgtacttttc gctgatcgac tggtcgcacc
    20641 cggactaccc cggctttctg cgcgattcca gtcggtacaa cgccggggcg gcgccggatc
    20701 ggtggcggcg gttcgtccaa ttctaccagg gccaactgaa cgagctcatg acgcagttcg
    20761 atccggacct gtactggttc gacggcgact gggagcattc ggcggaggaa tggcaggccg
    20821 ccggcgtgcg tgacctgatc cgccggcaca atcccgcggc gatcatcaac ggtcggctcc
    20881 agggtttcgg cgactacgac acgccggagc aaaacctccc tatcgagcgt cccgggtcgc
    20941 gctgctggga gctctgccag accatgaatg attcgtgggg ctaccagggc cgcgacacca
    21001 actacaagac gccgtccgag atcatcacga ttttcgccga tgtgatcagc atggggggaa
    21061 acctgctgct ggacatcggg ccgcaagccg acggcacgat tccggagaag cagcggaata
    21121 tcctgcggga actcgggcgc tggacagcca ggcacgccga ggcgatcttc gggacggttg
    21181 ccgggccgcc cccggggcat ttctacgggc ccgcgacgct ctcgaaagat tcgctgtcgc
    21241 tgtacctgtt tcttcccggg cggaccggcg gggacgtcgt cgtgaagggt ctgaattgcg
    21301 gggtcgccgg cgtcacggtg gtcggcagcg gcgagaccgt gacttacaga acggtcggca
    21361 agatctcgtg gagtccggtg cccggactca tcttcatcca tccaccggaa aaccaggacg
    21421 agtatatgac ggttctgaag gtgtcgctcg acagtccgct cgcgctgtac cgcggcaaag
    21481 gaggacttca gtgaaaaggc gcagtgttct ccttctccct ctcgtgctcg catgtctttt
    21541 ggccggcggg gcggcgaacg tggcgccggc gccttccctc acgctggcga ccgacggccg
    21601 cagcgagtat cggctctgtc tgccgccgca tgcctccggt caggtgacgg ccgccgccga
    21661 cacgcttgtg aagtacatcc gggaagtatc gggcgcgacg atcccgctgt cggaaggtgg
    21721 cgccgccgag ggcccgcgca ttgtgttcga gatcggcggg accgaggacc cgtcggccgg
    21781 gattgccggg ctcggcgacg acggcttcgc aattcgcagc gagggggacg atctctttct
    21841 tgcggcggaa acgccgtacg gtttgcagaa tgccgtgtac acgttcctcg agacctatct
    21901 cggctgccgt ctctactgcc cggcggtgcg gctaattccc cggcgcagca cgctcacact
    21961 cccggccatc aatgatcgcc aggttccggt gatcgcgttt cggatgctga atgtccgcga
    22021 ctcgggcttc acgacctggc acaagctcaa caggaacgat gactttgggc tcttcgtgca
    22081 cacgttccgg ctgctcgtgc cgccggaccg gtacttcccg gatcatccgg agtacttctc
    22141 gctcctcaaa ggtaatcgca cggcgagcgg gcagttgtgc ctgtccaacc ccgaggtgtt
    22201 ccggattgtc tgcgaaaagc tgcgggcgct gatgaacgag aagccgggcg cgcggttctg
    22261 gtcggtgtct cagaacgaca cctacgtgcc gtgcgaatgc agcgcctgcc gggcgatcga
    22321 cagcgccgaa gggtcgccct cgggctcgat cctggcgttt gtcaaccggg tcgccgatga
    22381 attcccggac atgaccatct cgacgctggc gtaccagtac tcgcgatcgg cccccgcgcg
    22441 gctcaagccc cggccgaacg tgaatatcat gctctgcagc atcgagtgca accggagcag
    22501 gccgctggcg gaggatccgt cgagtgcatc gttcgtcaag gatgttgcgg actggagccg
    22561 gctcacgcat aacatcttcc tgtgggacta cgtcatcaac ttccgcaacc tggtcagccc
    22621 gtttcccaat cttcgggtgc tgcagcccaa catccggttc tttgtgcaac acgggatcac
    22681 ctccgtgttc gagcagggtt tgtccggctt ccacggcgaa ttcgcggagc tgcggagcta
    22741 cctgatcgcg aaactgctgt ggaatccgga tatcgacgtg gattccgtca tggatgattt
    22801 cctgcagggg ttctatggag aggcggcccc gtatatacgg cagtacatcg acaccatgca
    22861 cgccgccatg gaggcatcgg gagaggggct cgatatctac ggctaccccg ttcggtcggc
    22921 cgacggctac ctgtcggcgg ccatgctgga tctctacggc cgcctctttg atcaggcgga
    22981 ggcggcggtg cagggggagc cggctgtcct ggcccgtgtc cggacggcgc ggttgccggt
    23041 gcaattcgcg gtgctggaac aggccaaggt cgcggcggcc ggggagaggg ggtgttttgt
    23101 gcgggcggcc gacggcagcc tgagcgttcg accgacgatc gactcgctcc tggcggcttt
    23161 tgtgcagggg tgcagggagg ccggcattcc cgcgctgtgg gagtacggta caacacccga
    23221 caagtacggc gcggcgacgc gcgcctttct ggagagcggc acgacaaccc atctcgcccg
    23281 gggcaaagcg gtccgccttg ggcggcccgc tagcccgaag tatcacgacg gcgaccgggc
    23341 ggcgttgacc gacgggttga aagcctggga cgattaccac atgcactggc tgggattcga
    23401 gggggaggat atggacgcga ccgtcgacct cggcaccgtc cggacgatct cccgcatcag
    23461 caccagtttc cttcaggaca tcaactcgtg ggtgttcatg cccgaaagcg tcacgttctc
    23521 cgtctccacg gacggccggg actatcgcct cgtcggcacg gttcgggata cggtgccggc
    23581 cgatcgcggc ggcaaggttg tcgcgccctt caatgttgcg tttgcagcga caaacgccag
    23641 attcgtccgg gtctatgcga gcagtctcaa aacctgcccg acctggcaca agggctcagg
    23701 cggtccggcg tggatattta tcgacgagat tgcagtggaa tgagcggccg gatatgaagg
    23761 ttctgaagtt cattctgcta tctgtgctcg cctgcatctc agccccggct gcaccggaag
    23821 cgcagccgta ccgcgatccg agcctgccgg tggaccgccg catcgatgat cttctctccc
    23881 gcatgacgcc ggaggagaaa ttccggcagt tgttcatgct agccgagaat cccggcgact
    23941 cgctcgaacg ccacgctcac ggcgtctttg gttttcagat cggcggcggc gaagatgctc
    24001 cggtcgccgt cgtcgccaac cgcatccagc ggcattttgt cgagcgcacg aggctgggga
    24061 tcccggtgat cctgtttgcg gaggctctcc acgggctggt gcagaagggg gcgacttcct
    24121 ttccccaggc aatcgcgctg gcggcgacat tcgatacggc gctgatgcac gacgtggccg
    24181 cgaccatcgc cgaggagtgc cgcgcgcggg gcatccggca ggtgctctcg ccggtcgtca
    24241 atatcgcgag cgatgcccgc tggggacggg tggaggagac ctacggagag gatccgtttc
    24301 tcgtgtcggc gatgggcgtt gctttcgtat ccgaattcga acggcgcggc gtgatcacga
    24361 cgccgaaaca cttcattgcg aatgtcggtg acgggggccg cgactcgtat ccggttcaca
    24421 tgaacgagcg gctgctgcgg gagatctttt tgccgccgtt tgccgcctgc atcggccggg
    24481 gcgggtcgcg gtcgatcatg gccgcataca attcgtacga tggatccccg tgctcggcga
    24541 gcaactggct caacaaccat cttctgaaag aagagctcgc cttccccggc tttgtcattt
    24601 ctgatgccgg cggggtcggg ggcgccaacg tgctgcactt cacggccgcg gactatcccg
    24661 aggccggaga acacgccatc aacggagggc ttgacgtcat attccagacc tcggacaatc
    24721 acgcggcgct gttcctgccg ccgtttctgg acggacgtat tcggagcgat gtgatcgacc
    24781 gggcggtgcg gcgcgtgttg cgcgtcaaat ttgagctggg gttgttcgag gatccgtatg
    24841 tcgatacggc cgacggcggc cttcggcaac gacgggaaac cggccgcgaa ctcgcccggc
    24901 gggcggcgcg ggaggcaatc gtgctcttga agaacgacgg gaacacgctg ccgctggaca
    24961 gcacgccggt ccggattgcg gtgatcggac cggacgcggc ggaggcgcgg ctgggaggat
    25021 acagcggtcc cggcaacgat cccgtgtcga tccttgaggg tattcgcgcg cgggccggcc
    25081 gggccggagc cgtgacctat gcccggggct gccgccggct cgacacggcc tatgtgacag
    25141 tgccgtcgac tgtcctctcg tgcgtggtcc gcgactcggt tcgggcgggg ctgctcggtg
    25201 cgtactatgc caacgccgcg ctgacgggcg cgccctcgct ggtccgggtc gatcccgaga
    25261 ttcagtttca atggactctc ttctcgcccg agccgggcct gctgccctat gactgttact
    25321 ccgcgcagtg gacgggaacc ctcacggctc ccgcgagcgg gctgttccgg atcggccttg
    25381 aggggagcga tggataccgg ctctatcttg acgattcgct tgtcctcgat cgccggcggc
    25441 aggtctcgag cgctgcgcgt ctggcggaat ttcggtttga gcagaatcgc cggtacgcga
    25501 tcaggctgga atttgccgaa ccggcgggca atgcgcggct gcggctggtc tgggatgtgg
    25561 gtgtgagcac cgacggcgat tcattgccgg ccgaggccgt ggcggcggcc gcggcctccg
    25621 acgtcgcgct cgttgtcgtc ggactcgagg agggagagtt ccgggaccgg gtatcgctgc
    25681 ctctgccggg ccgtcaagag gaacttatca ggcgggtagc ggcgaccggc accccggtga
    25741 tcgtggcgat tgtcggcgga agcgcggtga cgatgagccg gtggattgat tcggtcgcgg
    25801 ccgtcctcga ggtgtggtat cccggcgagg ccggggggga tgcggtggcc gacgtggtgt
    25861 tcggcgatgt caatcccggc gggcgactgc cgattacatt ccccctggcc gagggccagt
    25921 tgccgctcgt gtacaatcac aagccgaccg ggcgcggcga cgattatttc gatttgagcg
    25981 gccggccgct gtttcctttc gggtatggtc tgagctacac gcagtttgcc tacggagacc
    26041 tgcgttttga ccggggagaa atcggcatcg gcgataccgg gatcgtccga ttcacggtga
    26101 cgaatgtcgg tgcgcgcgag ggcgatgaag tggtgcagct gtatgttcgc gacctgctcg
    26161 catcggtggc gcgcccggtg attgagctca aaggattccg gcgcgtgcat ctgaagccgg
    26221 ggcagacggc gatggtcacc atggaaattc ctccggaggc gctcaccacg ctcgatgagc
    26281 gcctgaggcc gacgatcgag ccgggcgatt tccgggtgat gatcggatct tcatcgacgg
    26341 acattcgcct ccacggaacg ctgcgcgtgc ggccgtgagc ggatggcggc cggggacggg
    26401 aggccgctca gccggtcggt cgactctgac gtgcgccgtt ccgaaggccg gcatgacaaa
    26461 tgacccctcg acagaaaccc ctgttttgcc gaacgcgtcg caccgggaga cgcgcacctc
    26521 gcgcggccgc cgaggtgtgc atgcatgacc tcgtgatggc aaccggggcg cccgggcgtc
    26581 cgccgccgcg gtccgccgcc gaaccgacag cgcgccggtg gggggtcctc agtcctgatg
    26641 tgtcaccttt attctgcgcg tggccgtcct gtcgcccgcc gtcacccgca cccagtagta
    26701 gccgtcgggc tcggcgctga tatccaccgg aatcatcatc tcccggacct cggggtacga
    26761 acgttcgtac accgtccggc tgctgtcctc gctcaggacc gtcaccgcca ccgggtgcgg
    26821 atcgttgtca aagaaatcga tcgcgaacac tccccggttg cgaatgggaa acgggttgac
    26881 gatcggcagc ggctcggccg ccgccgcggg cggcgcgtcc tgggcgacca taaacgagtg
    26941 gcgaatcccc cggccgaaat cgggaacgaa cgacttgacc agccggccct ccccatcaag
    27001 aagccgcacg tagccgtacc cgccttccgg gttgaaccag aaatcgaggc cgtcgccggc
    27061 tgtgtcgtcc accgtcaatt cgtaacaccc cggcgccagg tgcagcgtgt cgcggtagag
    27121 ggctcccgcc ccgagcgtgc cgagcgcccg ctcccgcacg atcgcccccg atccgtcggt
    27181 gagccggtac gaattgtgtg cggagtcgtt gttgctgcgg agggcgagca ccaggtcggg
    27241 ggcgtaaacg gggacggccg ggccggtcga cgtcagttcg ttgtcggcgg ggtattcgtc
    27301 cggcctgccg ttgggcagct ccagccgggc ggcaaacggc cgatcgccgg cgatgctccc
    27361 cggcagggcg acttccgtcc gcgcctgcga ggcgagctcc ccggtccact cgaatgtttg
    27421 tggcgcctcc ggggggccgt aggatatcgc cacggccgtc agcgcttccc gcccgctgtt
    27481 tctgatcagc aggcgcgcgc cgcggcacga ggggttcagc cgggaatact catcggcgga
    27541 actcggaatg agaatttcct cgagggtggc gtcgtgcgcg gcccatggcg gagcataata
    27601 gaaaagatac gacgagagga cataggcggc gctcggattt ccgggattga cgtacggttc
    27661 catctcgagg gccaccgtgt gcgtcgagcc gggagtgacc ggaagatcat acagatcggg
    27721 ctgcacaatg gacccgggac accagttggc gcggtcgaag agccacgtgc ccgcctgtgg
    27781 aaacagcggg ttgtcgccgc aggtgcgcca gaccgggcgc tcgtcgacga gcacctcgtt
    27841 gacgatgatc cggcgcgtct tgcggcagaa ttccgcgcag ttctcctggt cgtccatgcc
    27901 gtggccggtc tggagaatgc gcaggcgggc gcgcgcggcc cccgtcggag cgatgaccga
    27961 aaccggggcg agggtgcgcg cgatcggtcg ggcgctgtcg ccgtacggga tcgatccctg
    28021 ccagagggtc tccatgccga ggcaggtcat cgccggtcgc ccctccgtga gggcgaagtc
    28081 aagcgtaatg acccagccgc ggtcggtgtt gctctcgtag ccggtatgca gaaactccac
    28141 ctcgaccgaa tcgtgcagca tccaggcgaa atcggtgatg tcgacgtgcc acgtgaaact
    28201 ccaggtggaa tcaaaccgcc agccgtaggg ggaaatcatg cgggcgacct cgagatcctg
    28261 cgccggcgcc gcctgcccgc cgacccggcg gagaaagatg tagtcgatat agtcccactc
    28321 gccgcagtgc aggccatccg ggcacgcata cgttacggag agcaccgccg tgcggaaggc
    28381 gacgctctcc ggcgggaaga ccgcccacgc gcacagcgga ttgctgcctg tggatgggtc
    28441 ggtcacggcc agcgtgcggt cgtgggagat gacgtgagtg acgggcgggg cgccccgggc
    28501 gagcgccggc ggcagtatgc cggcggtgca ggcccagacg aggaacggca aaacgaaagc
    28561 gcgcataatc gcggaacctc cagaggtgtt cgtccacagc ggcaatcagc ccaatatggc
    28621 acaccggagg cctgcgggcc accgttttcg ccgaccctcg gcagtgcctc cggcgattcg
    28681 ttgctcggac caagcagcgg cgcctcaacg gcttagcggg cagtccgcgg tcggcccggc
    28741 gggcaaccgc cccggcatgt catccgtaga cctccgctgt ccagattctg ctcgccgtgg
    28801 gcgcccccac gattgtcgga aggatcctgt tcagaaaaag cacagtcggt ccggccggtc
    28861 tcccgatccg gtccatttca tgggccgcgg ggatcccggt gagagattac cgattgacga
    28921 aagacggttc gcggttatat tgaaccaaca gcaggtggtc ctttgtcctg agtcgaactt
    28981 gatgcagaac gagaattctt cccggcggtg gatactatga aaaatcaaga cgtgaaggtc
    29041 tgtgcggcga tgagcggcgg gcgatggccg gccgtgagcg ccatcctgtt tctcctggcg
    29101 gcggctccgg ccctcgcgga atcgccggaa tcggtcgccg gaggaccgcc ccgaacggac
    29161 agcccggctc tcatcggcga tccgcaatcg gccccagccg agaacgtcta ctatataaat
    29221 ttcgactcca acctggaggg ctggtatttc ctcaaccacc cggacacggt gcaggagtac
    29281 taccccgatc aatacacctg cgatgaactc actcggatgc gcgcgaacgg cgcgagtttt
    29341 gccctctcct ggggctactt cgacgggagc ggcgccatga tggtcacgtg cccgtttcgc
    29401 ggctggtaca gggacagctg ctactactcg tggcgtatta agtcggtcca ggtcaatgtc
    29461 ccggacgagt gggacggcct ctaccgggtg gtggcgcggg tctacaaggg aaccggctcc
    29521 cggatatggg cgaaaatagg gtatcgaccg gccggcagcg ccaatacgta ctacctgccg
    29581 ggctggagcg agctccccac ggaaggctgg acgactctca ccctcagtga accatcccag
    29641 ggagcttttg ccgacctcga cgccgtgtcg attctgtttg gcgcctactc gtccgatggc
    29701 aacgtctacg tggactgggt gcaggcgtac gaggatcagc cggacgtgcc ggtgctggtc
    29761 gctcccgcaa acgatcagac tatccaggcc aaccttccca catttcaatg gaccggcaac
    29821 ggcgactact acacgctcca ggtggctacc gaccccaagt tcaccaacgt gctgatcgac
    29881 acttcataca tctacagcac cagctgcaaa gtgacgcggc cgctgccatt ctcggactac
    29941 gactacctgc ccgggggagg gggcgcccac gacccgcact actactggcg ggtcaaagcg
    30001 caccgggtcg gaccgagcgg ggtcagcctg tactccaatt acttccagtt cattatcaat
    30061 ggtccacatg aagtaccctc ggagtaccct tcgatcctgg cggcccgggg cgccctgcct
    30121 ccgtatatcg gggggaccgt tctcgtggcg cccggcacct ataccggcag agaaaattgc
    30181 agtatcggtc tcggcggcga gaagccggtc gacatcatcg ctaccggcgg ggcggccgta
    30241 accgttatcg actgtcagaa cacttactac gctttctcgt atgacgccta cacggccggg
    30301 gcccccgtcg tcgaggggtt cacgatccgg aacgcccggg ccgacgggag cgacgaggcc
    30361 gcgatcgtgt gccggacggg cgccggcacc atcaggaact gcattatcga aaacggcgcc
    30421 gggaccggtg tgcttgcgcg cagcggcggg attgcctatc tggtcaacac cgaaatcaga
    30481 aaccagaccg gcgatgccac cgaggctacc gatggcgggc gaatactggc gtccagctgc
    30541 cggttcatcg gcaacgatgg atacggcggg cgcctgatcc agagctccgc cggcgccggc
    30601 gcctgctcgt atgactcctg tctgttcgcg gacaatggga gcgagggcgg cggagggttg
    30661 tatgtcgggg cggcgacaag cctcggtatt accaattcga cgtttaccgg gaacgtcaac
    30721 ggcattgagt ttgcgactac gggccaatcg gtgaccacga tcgcgaattg cctcatcgcg
    30781 ttctcgaacg gacgcgggat cgactggaca tcatactccc atgatctgtc ggtgttgtgc
    30841 agcgatgcat acggcaacgg cggcggggat tgggtcaacc tggcggccaa cgagctcggc
    30901 ctgcgcggga atatccagcg ggatccgctc ttctgcgatg cgactgcggg cgactaccgg
    30961 ctggcggcgg gttctccctg cgccgcggaa tacagcgcct gcggcgggat cgggctgttc
    31021 gggatcggct gcatcctcga gctgggctgc gccatggcag cgtccgattc tggcggcccc
    31081 gcgccctgga ccatctcctt ttcggcctgg gccagccgcg gacaaccgcc ttacagctac
    31141 cactgggatt tcggagacgg cgggagcggc tcctccgaca ccgctctcca cacctacacg
    31201 gtatcgggcg tctacaccgt gcggctgact gtgaccgatc aactcggcgc cacgtgcgag
    31261 cagacttctg cggtgcagat cggaccggga ctgagctgca cggcggctgc cgatccgacc
    31321 agcggcccgg ctcctctggt cgtgtcgctg caggccgcgg gcagcggcgg agtccctccc
    31381 taccggtttt cctgggcttt cggcgacggc ggcggcagca cgcagcagaa cccgatacac
    31441 gagtactccg ccgagggagt ctacacctgc tctctcgcgg tctttgacca gcgcgggtat
    31501 tcctgcacgt ccgggctctc cattgtcgtg ggtgagccgc tgcagtgcgc ggcggcggcc
    31561 aatccggccc gcggcccggc gccgctgacg gtcaatttca gcgcgagcgc ctctggcg
//
LOCUS       DAOPOE010000027        31207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1889_length_31207_cov_6.354712, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000027 DAOPOE010000000
VERSION     DAOPOE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_31207_cov_6.354712"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..2660
                     /locus_tag="PKM94_05380"
     CDS             183..2660
                     /locus_tag="PKM94_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66107.1"
                     /translation="MAKKKKAGDAPPAAKRETSRLNIEQSPWFTPVAFAIIFIGLVIL
                     FRDFIFSRLMLAGSDMIQAGIFFRSFLVDYYHAHGAVPRWNPYIFGGMPFVDAFHGDI
                     FYPLSFLKYFGNIFRMLGWVQFWHIYLAGIFMYLAARQFRLSKIAALLAGVAYMFAPY
                     LVSFVAPGHDGKMFVTALFPLTVYFLDRGIARGSFFDFSMLGLVIGFIVLSPHPQMSY
                     FTLWALAAYMVFRLVFLFREKRRIRLLVRPAALTAYAVVIGLLLSAIQFYPGYTYTRE
                     FSPRADTKRGWEWATSWSLHEEEAVSLVIPEFAGVMTRGAQTFYWGKNAFKDNSEAVG
                     AVTFFLALLGAFFARRREAYFFAGLALFALLYALADTTPVFRLFYWLIPMVKSLRAPS
                     MIMFLFSFSAAMLAGMAVQRLRDARQAGNADPPGARFEWLLWGVPGGLFVLALLFSAA
                     GEGMLNLWTSLFYSEAAQTQVARGVSKLDVAYRNLPAIQSGAWLAFVFAALAAALIWA
                     YRSRRAGAAVLAVLVLVLMVDGIRLNSRFIEVMDPRQEWPENHPVAQFLLEQPGPFRV
                     MNFSVLRMDLLPYYGIPVVTGYHGNQLRWYDDLLGGPGAPHAGGDRMPAAANPRFLNL
                     VGARYLLVPGGTRLPDGFLGDRPVTELASSAQASVLQNDNAFPRAFLADRFRVVEDNA
                     AIDSLVLHGDENLREIVYLEKAPGLDIPRDSLGADSVWFVDYQPDTVVLGLQAAANKL
                     LVLTDNWYDAWQVMLDGRPAEILRADGSFRAVAVPAGTRAAMFVYKSDRYRLGKLVTV
                     LTALFLAGVVVFQVVRTRRRRGEEMAA"
     gene            2662..3396
                     /locus_tag="PKM94_05385"
     CDS             2662..3396
                     /locus_tag="PKM94_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOD66108.1"
                     /translation="MPAPHSQRALIIFPTYNERENIEKIVHAVLPLDPRINVLVVDDN
                     SPDGTGEIVDRLTRQEPKVHVLHREKKEGLGRAYLAGFQWALENGYDFIFEMDADFSH
                     GPEYIKDFLREIQRHDLVIGSRYINGVNVINWPMSRLLLSYFANVYTRWITGLPLRDA
                     TGGFKCFRRKVLESLDFDAIRSNGYSFQIEVSMRVWKKGFRIKEIPIIFYDRTSGESK
                     MSKRIVREAIWMVWWLRIKAMFGLLK"
     gene            3408..4274
                     /locus_tag="PKM94_05390"
     CDS             3408..4274
                     /locus_tag="PKM94_05390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD66109.1"
                     /translation="MSADKETVSVVIVTHNSMPPLEDCLRSLRAGANGTRLEIINVDN
                     RSADDSPEVVKRYFPQAKIIRNPSNVGFGRACNLAAEHATGDYLLFLNPDLQVDRNSI
                     ENLLEVCRRAERVGTAVGRMRFPDGSFQATCRRLPQFNNMLFSRGSIFARLMRTNGQY
                     TLPDFPEVTPVPAAAGTMMMIEKRLFRDVGGFDKRFFMFMEDVDLCLRLGLLGYRNYF
                     VPAAGAVHLWGRGSRVGKFRRNLHHHINVWRYFAKHYPNILSYALLPFALALNLLLVT
                     VLPVSQPANRRP"
     gene            4418..5377
                     /locus_tag="PKM94_05395"
     CDS             4418..5377
                     /locus_tag="PKM94_05395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOD66110.1"
                     /translation="MPEGPILDFERPIRELEKKISGMKDFSIGESIELSGEIALLERK
                     LERLREEVYSNLNRWQMVQLSRHPKRPYTLDYIRLMTTGFIELHGDRRFADDPAMVGG
                     LAWLDDQPVMIVGQQKGRDTKEKVRRNFGMAHPEGYRKALRLFRLAEKFDVPIVILID
                     TPGAFPGIGAEERGQAEAIAYNIREMFGLRVPIVIVIIGEGASGGALGIGVGDVVMML
                     QFSWYSVISPEGCAAILWRDAAKAPEAAEALRPTASDLMALKIVDKVIPEPSGGAHSD
                     PEGAAAAVRAEIQGALADLKALPVDRLLERRLAKYRVIGEFDE"
     gene            5398..5565
                     /locus_tag="PKM94_05400"
     CDS             5398..5565
                     /locus_tag="PKM94_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOD66111.1"
                     /translation="MPLSDKLLSKLACPKCRGPLSFDADQNRLLCSACRLSYPVREDI
                     PVLLVDEAEKL"
     gene            5581..6033
                     /locus_tag="PKM94_05405"
     CDS             5581..6033
                     /locus_tag="PKM94_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD66112.1"
                     /translation="MKLSKFCDETLVKFNLKSATKDDLIEELVDLVATSNMVKDRDQL
                     LTDVRERENLVTTGVGYGVAFPHAKTKSVKGIVIAFGRSKQGVDFDAMDHKPVHLFFL
                     IAAPEDAIGAHLNVMARLSFLMKSEKNRQKLLEANSPGDVLALMDNVE"
     gene            6056..6850
                     /gene="mreC"
                     /locus_tag="PKM94_05410"
     CDS             6056..6850
                     /gene="mreC"
                     /locus_tag="PKM94_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD66113.1"
                     /translation="MTWISTQFAKHWRNIQYTLVAVLSAALILWPALLDQTVRPVVLN
                     GLYYPFFKLRSAVELLRSQAAANQQLREALMAASLELAASREAVRENARLREQLGYTP
                     PPGRTILPAEVVAVAGAEMPVSAVIDRGSADFVRVDQPVINQYGLIGRVRSTTPRFAT
                     VQLLTDPANRVAGRIERSREMGIVRYLPGRGMILDNFPIFGDIAVGDTVVSSGLGGIY
                     PEGLVVGVVTDVHRGEHAAFADVAIDPAVNFQSIEELFLLRTEGRP"
     gene            6847..7332
                     /gene="mreD"
                     /locus_tag="PKM94_05415"
     CDS             6847..7332
                     /gene="mreD"
                     /locus_tag="PKM94_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOD66114.1"
                     /translation="MTVIPYLLYLLLVAFHEVILRDLTAIYGVTINLPVLLVLGVALY
                     KPEAAALWFGFAAGLAASAARPELSGWYALLTAGLAAAAWHVRERLNLEALLSKLLLV
                     FLGVLAYNLVAAAVGGLEGYWFHVYRLQIPGAVYTALVAWVFFLFKERRVTAQKLKAM
                     F"
     gene            7351..9240
                     /gene="mrdA"
                     /locus_tag="PKM94_05420"
     CDS             7351..9240
                     /gene="mrdA"
                     /locus_tag="PKM94_05420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD66115.1"
                     /translation="MTHPHLPLELRAKAALALVIAGALFLVAGLVRLQIFKYAQMAAQ
                     SEANRLRIVPIVPRRGVVYDRDGRRIIDNRPSYTLSVVPAEEVRGVTVPNLARLLGVD
                     TLEIRKRIRRNTISVFQPAPVQRDVSFETIAVIEEQVEQFPGVGYQVEQVRQYPVGLG
                     AEVFTGYVGEVSREELERYGNDNYPLGSMIGKKGIEKQYDQLLRGREGTRYMEVSATG
                     QLLGEYRGRQRQEATPGTDLTLTIDLDLQRACGEALDTLRCGAIVAADPRTGEILAMA
                     SYPAFDANVFSGVIPESLWTAISSDTLHPLLNRPLAGLYPPGSTAKVVTVGAGLEEGL
                     ITPSSTFRSCIGGYQFGNRFFRCWEKGGHGSLVAAHAIEQSCDVYMYQAGLKLGVDRL
                     SEYYIKCGFGRPTGINLPGELKGFIPTTEWYNRTYGKSGWTRAVVLNNSIGQGEVLAT
                     PLHLLQLYCGLAGNGIVQQLHIVRRFTTPAGDELEVSPKIKFALPFSESTLAILREGI
                     ELVVQGDRGTARSLRRKEYRIGGKTGTAQNPHGNDHAWFCGVAPLEAPEIVVVAIVEN
                     AGHGSTAAAPVVGKIIDAYMKKKRDPEAFFAEQQARADSLAAARALAGLSDTAAAPAR
                     AARGN"
     gene            9240..10463
                     /gene="rodA"
                     /locus_tag="PKM94_05425"
     CDS             9240..10463
                     /gene="rodA"
                     /locus_tag="PKM94_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOD66116.1"
                     /translation="MLDYRQLDWKLIGAVFALSAIGVVLIMTAQHHAESEYARTFYLR
                     QLLWLVIALAAAAAVLHLPYRLFDFAAYIFYAVAVLLLVAVLMFGATRMGATRWFSFG
                     PINFAPADVAKLALIFALARFFAYSKSPLLSGQRVAVSLGLTVVPMMLILKQPDLGTA
                     LTFPVVMYALWFWSGIPPLYLLLIASPLLSLVAASNAISWSIYMAVLLAVLILFKPGR
                     QLIFSVLIVVANLAFGAVMPLVWNRLEEYQQLRIISFVDPSQDPRGAGYQIIQSKIAI
                     GSGGLWGKGFLGGSQTKLDFLPERHTDFIFSVLGEEFGLWGALIVLALFFVFFYRGIR
                     IAARSRSRFLSYVSFGGVAIIFFQFLVNIGMTLGLMPVTGVPLPFLSYGGTSLVLSWV
                     LVAFLLKADYHWQEY"
     gene            complement(10480..11400)
                     /locus_tag="PKM94_05430"
     CDS             complement(10480..11400)
                     /locus_tag="PKM94_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOD66117.1"
                     /translation="MRSYCPMVLSLLMLAAPAAPAQQVPDSAFRPPIENPAFPAPNGP
                     VVLIDEGHHNFHTAGGRYYTFAELLRRDGYRVLPSGCRFTGDSLARGEILVVSNALHE
                     RNAQEWSLPTPSAFTPDEIAAVAAWVQGGGALLLIADHMPFPGAAAELAAAFGFHLHN
                     GFAVDTASPTGGTYVFRRADGTLRDHAITRGRNDRERIDSVASFTGEAFRADSAAGLM
                     VFGDGVVSLAPDTAWQFDANTAAVAVSGWCQGAVKVIGAGRVAVFGEAAMFSAQLAGP
                     NRTPAGMNSPAAPQNARFLLNVLHWLSGEL"
     gene            11514..12455
                     /locus_tag="PKM94_05435"
     CDS             11514..12455
                     /locus_tag="PKM94_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD66118.1"
                     /translation="MRRHSQERTGRALAILAFGALCISFAAVFVTLLGAGRLGPGAIA
                     FWRTFFGAGILFVWAALAGHRLGLPWALKRWTILAGVLFAGDLFVWHRSVLLVGVGMS
                     TIFANTQVFGTAVLSYFIFRERLTLGFFAAAAGAILGLILLIGVGSDIAFTAPYLRGV
                     LLGLLTAVFYAHYIVTIKIAGQREGPPPSFITLMAWVSSAAAFFLLIASLLYERHEFF
                     PPDWTSVGILVLLALVAQALGWWAISQALPRLDAHRSGLALLLQPVLAAVWGALFFAE
                     RLTPLQTLGAAITLLGIYLGTVRRPAGQQSRTTPAGT"
     gene            12477..13157
                     /locus_tag="PKM94_05440"
     CDS             12477..13157
                     /locus_tag="PKM94_05440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOD66119.1"
                     /translation="MESNPRTGSLSEKTAAWLKHIEQVNRHPMTLNRGSSALLVVDMQ
                     NFFLDPNSAAFTCGGPPIIPNVILLIAAFRAQNRPVIYTKHVHHPSGHDAGIMAWWWK
                     GMCLEGTPESEIHPDLAPAAGEKVIAKHRYSAFYGTDLEIVLRGLRITDLVITGVMTN
                     LCCESTARDAYFRDYRVFFPADAAGSVSEELHLAALMGLAFGFAHVTRTADLLRDLTE
                     SRPARTEP"
     gene            complement(13246..14526)
                     /locus_tag="PKM94_05445"
     CDS             complement(13246..14526)
                     /locus_tag="PKM94_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOD66120.1"
                     /translation="MADVRTTHRGPVGYSTYSDQMIYDFESSYGAHHYSRQHLVVRKA
                     EGIWLHDEKGDRYLDCLAAYSAVNQGHHHPRIVQAVVEALQSGYGSVISNVVYTDPLG
                     VFLKKLATLVPQMGPRFGDNGNKVLPKNGGVESVETAVKLARYYGYKEKGIPDGQQEI
                     VVFNNNFHGRMITTVSFSSNAYYKQGFGPLTPGFRTVDFGSLDKVEKAITKNTCGILV
                     EPMQGEGGMYQAPAGFLKGLRELSDRHNLILLFDEIQVGLGRTGKMFAFEHEGVVPDG
                     IILGKALSGGLVPLSCFVTNAKLMDIVFQPGRDGSTYGGYPLACVAGNAALDVIVEER
                     LPENADQVGAYLAEQIRAIGARSPHVKEVRARGLFIGIEVKNGDAMKYCREMLGARLM
                     ANDSHGHTIRISPPLIITKKDADFIAAGLERVLV"
     gene            14931..15809
                     /locus_tag="PKM94_05450"
     CDS             14931..15809
                     /locus_tag="PKM94_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOD66121.1"
                     /translation="MPKIVDNVELGPMIWKMRVAVPRLVAKAKAGQFVILRVNNEGER
                     VPMSIAGLDQANGLLTIVYQVVGKTSALMTTVAPGHELSDCVGPLGIPTHVDTWGTAC
                     VVGGGIGIAPVYPIAQAFKKAGNKVVTIIGARTRDLLFYEEEHKAVADEFYPCTDDGS
                     YGHHGFVSDVLKQLLDRGAPIKHIMAIGPVPMMRVVSKLTAPYGVSTWVSLNPLMIDG
                     TGMCGGCRVKIGEETKFACVDGPDFDGHLVDFDLLTSRLTAYREQERRAMKRLLDDPN
                     CRLTKSVQAFAYPDEK"
     gene            15904..17298
                     /gene="gltA"
                     /locus_tag="PKM94_05455"
     CDS             15904..17298
                     /gene="gltA"
                     /locus_tag="PKM94_05455"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOD66122.1"
                     /translation="MRIPRQKMPEQQAEVRVGNFKEVPFGFTIDLALTEAQRCLECPK
                     APCIKGCPVEVDIPGFIRLILEKDFAAAARKIKETNSLPAVCGRVCPQEEQCEIACVL
                     HKKFQPVAIGALERFVADYERENHLVQLPRKQPPTGRKAAVVGAGPAGLTVAGDLILL
                     GHEVTIFEALHKPGGVLVYGIPEFRLPKAIVQSECDYLRDLGVEFRNSTVIGKMDTID
                     ELFEMGYDAVFIGTGAGLPNFMGVPGENLIGIYSANEYLTRSNLMRAFEEDSYDTPII
                     RGRNVAVLGGGNTAMDAVRTALRLGAENAYIVYRRSEAEMPARKEEIHHALEEGVQFH
                     MLTTPIRFLGDERNRVRAMECLQMELGEPDASGRRRPVPIEGSNFTMEVDLVVVAIGN
                     GSNPLIPQTTPGLKTNRWQNIVVDEETMQTSREGVYAGGDIVTGGATVILAAGAGKKA
                     ARAMHKYMMEKVRT"
     gene            17403..18401
                     /gene="queG"
                     /locus_tag="PKM94_05460"
     CDS             17403..18401
                     /gene="queG"
                     /locus_tag="PKM94_05460"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOD66123.1"
                     /translation="MLTSELVKRLAAAAGFDLCGVTSPEVIPEARDRFLRWLAEGRHA
                     EMHWIARSTHRRVDPRELLPSVKSVIMLGVNYYNPNSPDRPPGHGRVSRYARGRDYHK
                     VIRRLTLHLIQRLQEQVAPQRHDFIWYVDYGPFLERAYAARAGLGFIGKNSMLINRTF
                     GSWVFLSEILTSLALDPDEPCGGRHGACGECTACIDACPTGAIVGPAMIEAAKCISYL
                     TIERPRAIPEALQSRMGALIFGCDICQQVCPYNRRATPTPHRELLPAAGAGEFVDTRR
                     VLALRDREEFLAFAAGTPLVRPRLEGLQRNARIVLENESRRTREGGGAAGGGEVPQ"
     gene            18626..20350
                     /locus_tag="PKM94_05465"
     CDS             18626..20350
                     /locus_tag="PKM94_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66124.1"
                     /translation="MKPGAFSISAGRTEAACQRALRRLERNAVLPRIMVRDAGLFSRQ
                     PAVRDKIANRLGWVDVAGTIRKRVAAIESFGHDVIKAGFRDVVLLGMGGSSLCPDLFG
                     RIFGRHRSLDSFAVLDSTDPRAVTAALRKVQLDRTLFIVSSKSGATVETRSQMICFLD
                     RLRQEGFSRQGRQFVAITDSDSALHSAARSEGFRRIYLNPPDIGGRYSALSYFGLVPG
                     FFAGVNLAALLDDAYAMEKILRARDDETNPAAALAALMAAGAESGSDKMTFIASRGLA
                     PLVPWIEQLVAESTGKKTRGVVPIDGEPLGAAADYRDDRMFVTLRLASEKPPLPENLR
                     AAFRKKGFPIVDLSLPSYDALGGQFLLWEVAVAAAGYLMGINPFDEPNVTESKRNTER
                     ILDQYRESGQFADLAAGRTYGRLTLVSHGGESHLRPSDLADLKRLLTRFLAGLRPPEY
                     VALLCYSKEERALDRQFAGMRAAIRRRRGVATLCGYGPRYLHSIGQLYKGGPDVGRFI
                     VFLRADYDRLEIPGRFYDFGQLITAQAIGDVRALASRDLPVLVFAVKGNVTTAVKEFA
                     DVLHAVCQ"
     gene            20364..22301
                     /locus_tag="PKM94_05470"
     CDS             20364..22301
                     /locus_tag="PKM94_05470"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOD66125.1"
                     /translation="MKDFDPWRGFTSSALSPEWRKKLSETARRCRAQILKMTSLAASG
                     HPGGSMSSLELYLTLYHMARVDPRNPMRDDRDRIIVSHGHTSPGAYTALAAAGFFDPA
                     AAMHGFRQAGSPFEGHVERSVPGIEWDTGNLGQGLSVGIGKALYARLSGLNFHTFVIM
                     GDGEQQKGQVGEARRVAAKFALSRLTAIIDRNRLQISGRTVDILPQDLAAEWEADGWQ
                     AVEIDGHSFDQIYGELHRAVTNEGRPIVIIANTVMGKDVGFMENDEAFHGAPVAPDML
                     TPALALLGDHGKDLPALRELRKQGPPPAFKIPRPPYPPVETGQPATYGADVKMDNRTA
                     FGKALLAVADLNLPREDFVMAVFDCDLAGSVKTAAFGQKYPDNFFQCGITEHNTAAIA
                     GSLSAERAVSIWADFAVFGVDETFNQARLNDINHANLKLFCTHTGVQVGEDGKTHQCI
                     DYFALLNSTFGWRVITPADPNQTDRITRWVLSRPGNFAVFMGRSATPIIPDADGRPFF
                     GGDYEYRYGRMDVLRRGERLALVAAGNMCAIALDAWQRLSREGVGISLISVADWSDFH
                     ADDLQMLAAHEHLVVLEDHNVKTGLGTALAAELFQAGARSTLTKMGVTAYASSGPPAD
                     LFRLLGLDPASVVARVKAILG"
     gene            22494..23465
                     /locus_tag="PKM94_05475"
     CDS             22494..23465
                     /locus_tag="PKM94_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOD66126.1"
                     /translation="MFVASVSRRTVRLAAVTEAQGRVTLKDQARFDAADFDSFETILQ
                     RYFSRRRTEGALFFVAVAGLPVGSTVSSALLPWAIDGDSIRAHFSFDVVRLVNEHLAT
                     ARGLFDLPPDALFTINDQPAVPGNIGLMAVDELLAEVLMVRRDNGYLSFLTNGGHTGL
                     APATQLEAELWQYLYAQRDVVEVGDVVSSAGLVRLVDFYLESHGHEVPEWLQNAPDPP
                     SRIVETALAGKQQVAVDAVDLFVDCFAGEAANLALRGATTGGLYLAGRVTTELLPSLD
                     QGRFLDNYLKRGAAEEKLRPIPIRVILDSAAPLRGVARMALEFEHPQ"
     gene            23462..24130
                     /locus_tag="PKM94_05480"
     CDS             23462..24130
                     /locus_tag="PKM94_05480"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOD66127.1"
                     /translation="MTRAVAFLHGVYRRRDLPFYRALCRGRLTIAVDGGYRFFRMAGL
                     FPDILLGDFDSLKRLPANLPPRTRVLRFPSRKDKTDAHLAIECCRERGLGAIDLVMPG
                     LGEIDHALGVVFLLGLRDRPAARSGRADIRMVSPQCEIRLVADSRITYRRRGGDLVSV
                     LPLSARIRLTTEGTEYDVTEAWLQFGDTRGLRNRIVRARATVAVRGQALVVRRFSGGL
                     AARP"
     gene            24331..24401
                     /locus_tag="PKM94_05485"
     tRNA            24331..24401
                     /locus_tag="PKM94_05485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24363..24365,aa:Gln,seq:ctg)
     gene            complement(24431..25555)
                     /locus_tag="PKM94_05490"
     CDS             complement(24431..25555)
                     /locus_tag="PKM94_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD66128.1"
                     /translation="MDMLLNFLQQGAFFNFSWGNLVMLLAGCGFLFLALTKGYEPLLL
                     VPIGFGILMGNIPLAPGQEIGIYEEGSVLSILYKGVSEGWYPPLIFLGIGAMTDFSAM
                     LASPRLILLGAAAQLGIFVTFLGALWLGFDADSSASIGIIGGADGPTAIFLTSKLAPH
                     LLGAIAVSAYSYMALVPVIQPPIIKLLTTRNERLIRMKPAPPVSKRLRILFPIVAFII
                     TCFIAPGALILLGMLFFGNLLKESGVTERLAATARTAFIDIVTILLGLTVGASTDASR
                     FLTPDSIKVFVLGAASFAIATAGGVLFAKVMNLIARNKINPIIGAAGVSAVPHSARVC
                     QVMGAREDPNNYLIMHAMAPNVAGVIGSAVAAGVLLATFG"
     gene            complement(25584..25967)
                     /locus_tag="PKM94_05495"
     CDS             complement(25584..25967)
                     /locus_tag="PKM94_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOD66129.1"
                     /translation="MKLRITVHGVAYEVDVEVLDAGEGFPAAAPRPRPRQSLTAGGPP
                     APAPTPAAPAAAGQGAVASPIAGTVVEIKAKVGMAVKQGDILLSIEAMKMKTAIAAPT
                     AGKVKAIPVAVGDSVRENQTLVEFE"
     gene            complement(26006..26353)
                     /locus_tag="PKM94_05500"
     CDS             complement(26006..26353)
                     /locus_tag="PKM94_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOD66130.1"
                     /translation="MADGAEFNVALFVVVGLLVVFAALAIIVTAVSLFGRLDKRWRAR
                     EAQADEAAFGKEPTIDTTTLVLIAAAAATVTQGRFHITRIRRLLPVDGGTRTWSASGR
                     AVLMGSHVVPRRR"
     gene            complement(26368..27918)
                     /locus_tag="PKM94_05505"
     CDS             complement(26368..27918)
                     /locus_tag="PKM94_05505"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOD66131.1"
                     /translation="MADPRIEALRRKRGQMTAGGGPQAVDKQHERGKLTARERIDLLF
                     EPGTFRESLLFIQHRQTAFGMAGKEFPGEGVVCGHGIIDGRPVYVASQDFTVGGGSVG
                     EMTGRKISEAMDAALKTGDPLVMINDSGGARIQEGVDSLQGYGHIFYRNVLLSGVVPQ
                     ISIIAGPCAGGAAYSPALTDFIIQVRHQGQLYITGPLVIKQVTGEEITAEQLGGAESH
                     AYYSGVVHFIAENDEHALALARRLLSYLPSNNTEDPPFDAALHEEAIGPDPAMNEIVP
                     EDPREPYDMREVIAGILDQGDFLEVMEHFAPNILIGFGRLAGYSIGVVANQPVQKAGV
                     LDIDSSDKAARFIRFCNAFNIPILTLVDVPGFMPGVQQEFSGIIRHGAKMLFAYAAAT
                     VPKMTVVIRKAYGGAYLAMCAKSMGADLLCAWPSAEIAVMGAEGAVNVLYRKEIGEAA
                     DPEEERARRITEYKDMFANPYAAAAHGLVDEILEPADTRPYLAQALEVLKAKRELRPA
                     KKHGLIPL"
     gene            complement(28167..29870)
                     /locus_tag="PKM94_05510"
     CDS             complement(28167..29870)
                     /locus_tag="PKM94_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOD66132.1"
                     /translation="MASDERATSNFIRDIIDDDLAKGTYDGRVHTRFPPEPNGYLHIG
                     HAKSILLNYGLAQTYGGKFNLRFDDTNPTKEEEEYVNSIIEDVRWLGADWEDRLFFAS
                     DYFPQLYEWAVQLIKAGKAYVDDLTPEEIRATRGTLTEPGQESPFRNRPIEENLDLFE
                     RMKNGEFPNGAKTLRAKIDMGAGNVNLRDPVMYRVLHARHHRTGDAWCIYPMYDWAHG
                     QSDSVEGITHSICTLEFEDHRPLYNWFLDQLGIYHPRQIEFARLDLTYTVMSKRKLLR
                     LVQEKYVDGWDDPRMPTISGLRRRGYTPESLRDFAERVGVAKKKGSVADLALLEHCLR
                     DDLNKRARRVMAVLDPLKVVITNYPEDRVEEIEAVNNPENAAEGTRMVPFAREIYIER
                     DDFMEHPPAKFFRLSPGAEVRLKHAYFIRCQEAVKDAAGKIVELRCTYDPASRGGEAP
                     DGRKVKGTLHWVSAAHAFEGEVRWYENLFSNEFPEEIAPEEAAAGKDFTAHINPHSLR
                     ILTGCRLEPMLKEARPGDRFQFLRHGYFCVDNKFSAPGRPIFNRTVGLRDTWAKLAAK
                     D"
BASE COUNT         5119 a        10878 c         9423 g         5787 t
ORIGIN      
        1 gattctcggg gccgcgccac gccatgatcc ggggggacaa aaaaggttgc tttgccgatg
       61 gtttgtagtt atgcttggcc tcgggtatcg gcgcggccga aacgccctaa cagtttgtct
      121 cacaagcccg tggccgtgtt cgacacttcc cggaagctga ggcgcagaga gcggagaatc
      181 gtttggccaa aaagaaaaaa gcgggggatg ctccccccgc cgcaaagcgc gaaacgagtc
      241 gcctgaatat cgagcagtcg ccctggttca ccccggtcgc cttcgccatc atcttcatcg
      301 ggctcgtcat cctcttccgc gacttcatct tctcccgact catgctcgcc ggatcggaca
      361 tgatccaggc cgggattttc ttccgctcct ttctggttga ctactaccac gcccacggcg
      421 cagtgccgcg ctggaacccc tacatcttcg gtgggatgcc ttttgtcgat gcctttcacg
      481 gcgacatttt ctaccccctg tcgttcctga aatacttcgg caacatcttc cgcatgctcg
      541 gctgggtgca gttctggcac atttatctcg cgggcatttt catgtacctt gccgcccggc
      601 agtttcggct ctcgaaaatc gccgccctgc tggccggcgt ggcgtacatg ttcgctcctt
      661 acctcgtgtc gttcgtcgcg cccggccacg acggcaagat gtttgtgacc gccctgtttc
      721 cgctgacggt gtatttcctc gaccggggga tcgcccgcgg gtcctttttc gacttctcga
      781 tgctcgggct cgtgatcggg ttcatcgtac tctcgccgca cccgcagatg tcgtatttca
      841 cactctgggc gctggcagcg tacatggttt ttcgacttgt atttctcttt cgggagaagc
      901 gcaggatccg cctgctggtg cgtccggcgg cgctcaccgc ctacgccgtg gtgatcggcc
      961 tcctcctgtc ggccatccag ttctatcccg gttacactta cacccgggag ttttcgccgc
     1021 gcgccgacac caagcgcggc tgggagtggg cgacgtcgtg gtccctccat gaggaggagg
     1081 ccgtctcgct ggtgatcccg gaattcgccg gggtgatgac ccggggggcg cagacgttct
     1141 actggggcaa gaacgctttc aaggacaatt ccgaggcggt cggggcggtg acattcttcc
     1201 tggcgctgct gggcgccttt ttcgcccgcc gccgcgaggc ctacttcttc gccggcctgg
     1261 ctctcttcgc cctcctctat gctctggccg acaccacccc ggttttccga ttattctact
     1321 ggctcatccc gatggtgaaa tcgctccgcg ccccgtccat gatcatgttc ctgttttcgt
     1381 tctcggcggc gatgctggcc gggatggcgg tccagcgtct gcgcgatgcc cgtcaggccg
     1441 ggaatgccga cccgcccggc gcgcgtttcg agtggctcct ctggggcgta cccggcgggc
     1501 tgttcgtcct cgcgctgctg ttcagcgcgg cgggggaggg gatgctgaat ctctggacct
     1561 cgctgttcta cagcgaggcg gctcagaccc aggtggcccg gggcgtgagc aagctcgacg
     1621 tcgcctaccg caatctcccg gccatccaat ccggggcctg gctcgcgttt gtttttgcgg
     1681 cgctggcggc cgcgctgatc tgggcctacc gctcgcggcg cgccggcgcg gccgtgctgg
     1741 ccgttctcgt gctggtgctg atggtcgacg gaattcgtct caacagccgc ttcatcgagg
     1801 tcatggatcc ccggcaggag tggccggaga accatccggt cgcccagttc ctcctcgagc
     1861 agcccggccc gttccgggtc atgaacttca gcgtcctgcg catggatctg ctcccctact
     1921 acgggatccc ggtggtgacc ggctaccacg gcaaccagct ccgctggtac gacgacctcc
     1981 tcggcggccc gggggcgccc cacgcgggcg gcgaccggat gccggcggcg gccaatcccc
     2041 gcttcctcaa tctcgtgggg gccaggtacc tgctcgtacc cggcggcacg cggctgcccg
     2101 acggttttct gggcgaccgc ccggtgaccg agctcgcctc cagcgcccaa gcctcggtcc
     2161 tccaaaacga caacgctttc ccccgcgcgt tcctggccga ccggttccgc gtggtcgagg
     2221 acaacgccgc catcgactcg ctcgtcctcc acggcgatga gaacctccgc gagatcgtct
     2281 acctcgagaa agcccccggc ctggacattc cccgcgactc gctcggcgcc gactccgtct
     2341 ggtttgtcga ctaccagccc gacaccgtgg tcctggggct gcaggcggca gcgaacaagc
     2401 tccttgtcct gaccgacaac tggtacgatg cctggcaggt gatgctcgac ggccgccccg
     2461 ccgagatcct gcgcgccgac ggcagtttcc gcgccgtggc cgtgccggcc gggacaagag
     2521 ccgccatgtt tgtgtataaa tccgataggt accggctggg gaaactggtc accgtcctga
     2581 ccgccctctt cctcgccggt gtggtcgttt tccaggttgt gcgaacgcgc cgccgccgcg
     2641 gcgaggagat ggctgcctga tatgcccgcg ccccactccc agcgtgcgct gatcatcttc
     2701 ccgacttaca acgagcggga aaacatcgaa aagatcgtcc acgcggtcct gccgctggac
     2761 ccacgcatca atgtgctcgt ggtcgatgac aactctcccg acggcacggg ggagattgtc
     2821 gaccgcctga cccggcagga acccaaagtc cacgtccttc accgcgaaaa aaaggaaggg
     2881 ctgggccgcg cctatctcgc cggtttccaa tgggcgctcg aaaacggtta cgactttatc
     2941 ttcgaaatgg atgccgactt ctcgcacggg ccggagtaca tcaaggattt cctccgcgag
     3001 attcagcgtc acgatctggt gatcggctcg cgctacatca acggtgtcaa cgtcatcaac
     3061 tggcccatgt cgcgcctgct tctgtcctat ttcgccaacg tctacacacg ctggatcacc
     3121 ggcctgcctt tgcgcgacgc caccggcggg ttcaagtgct tccggcggaa agtgcttgaa
     3181 tcgcttgatt ttgatgctat ccgctccaac ggctactcgt tccagatcga agtttccatg
     3241 cgcgtctgga aaaaaggctt tagaatcaaa gaaatcccca ttatctttta tgatcggacc
     3301 tccggggaat ccaaaatgtc caagcggatt gtccgggagg ctatctggat ggtgtggtgg
     3361 ctgcgcatca aagcgatgtt cggcctgctg aaatgagaga cggggacatg tcggcggata
     3421 aagaaaccgt atcggtcgtc atcgtgacgc ataactccat gcccccgctg gaggactgtc
     3481 tgcgcagcct gcgcgccggc gctaacggca cccgcctgga gattatcaac gtcgataacc
     3541 gctccgccga cgattcgccc gaggtggtga aacgctactt cccgcaggcc aagattatcc
     3601 gcaacccctc caatgtcggg ttcggccgcg cctgcaacct ggctgccgag cacgccaccg
     3661 gcgactacct tctgttcctc aaccccgatc tccaggtcga ccggaattcg attgagaacc
     3721 tcctggaggt gtgtcggcgc gccgagcggg tgggcacggc cgtggggcgg atgcggtttc
     3781 ccgacggttc gttccaggcg acctgccgcc gcctgccgca attcaacaac atgctcttct
     3841 cgcgcgggtc gatcttcgcg cggctcatgc gcaccaacgg ccagtacacg ctcccggatt
     3901 ttcccgaggt gaccccggtg ccggcggcgg ccgggacgat gatgatgatc gagaagcggc
     3961 tgtttcggga cgtcggcggg ttcgacaagc gcttcttcat gttcatggaa gacgtggatc
     4021 tctgtctgcg gctggggctc ctgggctacc ggaactactt tgtgcccgcc gcgggggccg
     4081 tgcacctgtg ggggagaggc agccgggtcg gcaagttccg ccgcaacctc caccaccata
     4141 tcaacgtctg gcgctacttc gccaagcact acccgaatat cctgtcctat gccctgttgc
     4201 ccttcgctct ggcgctcaac cttctcttgg tgacggtgct gccggtgtcg cagccggcca
     4261 accgccgccc ctgacacccc gcccttccca tcgctccatt ttttgaggtt gttatttcat
     4321 tgccgatcgt tatcttaaaa acttgaccgc gtcccgcggg gcggccgggg aagcctgtgc
     4381 gcgccgcgga cggcacaagt agcgacgggg actgcgtatg ccggaaggcc cgatactgga
     4441 tttcgagaga ccgatccgtg agctggagaa gaagatctcc ggcatgaagg atttcagcat
     4501 cggcgagagc atcgagctgt cgggggagat tgccctcctc gagcgcaagc tcgagcgcct
     4561 gcgggaggag gtttactcca acctgaaccg ctggcagatg gtgcagctct cgcgccaccc
     4621 caagcgcccc tacaccctcg actacatccg cctcatgacc accgggttca tcgagctgca
     4681 cggcgaccgg cggtttgccg atgatccggc catggtcggc gggttggcct ggctcgatga
     4741 ccagcccgtc atgatcgtcg ggcagcagaa ggggcgcgac accaaggaaa aagtgcggcg
     4801 gaatttcggc atggcccacc ccgaggggta ccgcaaggcc ctgcgcctct tccgcctggc
     4861 cgagaagttc gacgtcccga tcgtcattct catcgacacc cccggcgcct tccccggcat
     4921 cggcgcggaa gagcgcggtc aggcggaggc gatcgcctac aacatccgcg agatgttcgg
     4981 gctccgggtg ccgatcgtca tcgtcatcat cggcgaaggc gcctccgggg gggcgctcgg
     5041 aatcggcgtg ggcgacgtgg tgatgatgct tcagttctcg tggtactcgg tgatttcgcc
     5101 ggagggatgc gcggccatcc tctggcgcga cgcggccaaa gcgcccgagg cggccgaggc
     5161 cctccggccc accgcctccg acctcatggc gttgaagatc gttgacaagg tgattcccga
     5221 gccgagcggt ggggcgcaca gcgatcccga gggcgccgcc gccgctgtcc gggctgaaat
     5281 ccagggcgcg ctcgccgatc tcaaagctct cccggtcgac cggctgctcg agcgccggct
     5341 cgccaagtac cgggtgattg gagaatttga cgagtaagta gacgtggagt tgtgcatatg
     5401 ccgttgtccg acaaactgct ttccaaactg gcctgcccca aatgccgggg cccgctctct
     5461 tttgacgccg accagaaccg gctgctctgt tccgcctgcc ggctctcgta cccggtccgc
     5521 gaggatatcc cggtcctgct cgtcgatgag gcggaaaaac tgtaggcaaa ggtaggggca
     5581 atgaaactgt ctaagttctg cgacgaaacc ctggtcaagt tcaacctcaa gtcggccacc
     5641 aaggacgacc tcatcgagga actggtcgac ctcgtcgcca cctccaacat ggtcaaggac
     5701 cgcgaccagc tgctgaccga cgtccgtgaa cgcgagaacc tcgtcaccac cggggtcggc
     5761 tatggggtcg ccttccccca cgccaagacc aagtcggtca aaggcatcgt gatcgcgttc
     5821 gggcgaagca agcagggggt ggacttcgac gccatggacc acaagccggt ccatttgttc
     5881 ttcctgatcg ccgcccccga ggacgccatc ggcgctcacc tgaacgtgat ggcgcggctg
     5941 tcctttttga tgaagtccga gaagaaccgg cagaaactgc tcgaggcgaa ttcgccgggg
     6001 gatgtactgg ccttaatgga taacgtcgaa tagccgccct gccgatcatc ccggtatgac
     6061 ctggatttcg acgcagtttg ccaagcactg gcgcaacatc cagtatactc tcgtggccgt
     6121 tttgtcggcc gcgctcattc tttggcccgc cctcttggac cagacggtcc ggccggtggt
     6181 gctcaacggt ctgtactacc cgtttttcaa acttcgcagc gccgtggaac tgcttcgctc
     6241 gcaggcggcg gccaaccagc aactgcgcga ggcgctcatg gccgcgtcgc tcgaactggc
     6301 cgccagccgc gaggcggtcc gcgagaacgc ccgcctgcgc gagcagctcg gctacacccc
     6361 accccccggc cgcaccattc tccccgccga ggtggtcgcc gtggcgggcg ccgagatgcc
     6421 ggtgagcgcg gtgatcgacc gcggctcggc cgacttcgtc cgcgtcgacc agccggtcat
     6481 caaccagtac ggcctcatcg gccgggtgcg ctcgaccacc ccgcgcttcg ccaccgtcca
     6541 gctcctcacc gaccccgcca accgcgtcgc cgggcggatc gaacgcagcc gcgagatggg
     6601 catcgtccgc tacctgccgg gccgagggat gatcctcgac aacttcccca tcttcggcga
     6661 catcgccgtc ggcgacacgg tcgtctcctc cggtctcggc ggaatctacc ccgagggtct
     6721 cgtggtcggg gtggtgaccg acgtccaccg aggggaacac gccgcttttg ccgacgtcgc
     6781 cattgacccg gccgtgaatt tccagtccat cgaggagctc ttcctcctgc gcacggaggg
     6841 gcggccatga ccgtcatccc ctacctcctc tatctgctcc tggtggcttt tcacgaggtg
     6901 atcctgcgcg acctcacggc catctacggc gtcacgatca acctgccggt tcttttggtc
     6961 ctcggggtgg cgctgtacaa gccggaggcg gcggccctgt ggttcggctt cgccgccggg
     7021 ctcgcggcct cggccgcccg gcccgaactg tccggctggt acgccctcct gacggcgggg
     7081 ctggccgccg ccgcctggca cgtgcgcgag cggctcaatc tcgaggctct cctgtcgaaa
     7141 ctcttactgg tgttcctcgg cgtcctggcg tataacttgg tggccgcggc cgtggggggg
     7201 ctggaggggt actggttcca cgtctaccgg ctgcagattc cgggcgccgt gtacaccgcc
     7261 ctcgtcgcct gggtgttctt tctcttcaag gagagacggg tgacggcgca gaaactgaaa
     7321 gcgatgtttt gacgcggttt cccgcaggtt atgacgcacc cgcatttgcc actggagctt
     7381 cgcgcgaagg ccgccctcgc ccttgtgatc gcgggcgccc tgtttctcgt cgccgggctg
     7441 gtccgccttc agattttcaa gtatgcccag atggccgcgc agtcggaggc caaccgcctc
     7501 cgcatcgtgc cgatcgttcc ccgccgcggt gtcgtgtacg atcgcgacgg ccgccggatc
     7561 atcgacaacc ggccctccta caccctctcc gtcgtccccg ccgaggaagt ccgcggggtg
     7621 acggtgccga atctcgcccg cctcctcggt gtcgacacac tcgagatccg caagcgcatc
     7681 cgccgcaaca ccatcagcgt tttccagccc gccccggtcc agcgcgacgt ctccttcgag
     7741 acgatcgccg tgatcgaaga acaggtcgag cagttcccgg gtgtcggtta ccaggtggaa
     7801 caggtgcggc agtacccggt cggtctcggg gccgaggttt tcaccggcta cgtgggcgag
     7861 gtgtcgcgcg aggaactcga gcgctacggc aatgacaact acccgctcgg cagcatgatc
     7921 gggaaaaagg ggatcgagaa gcagtacgac cagcttttgc gggggcggga aggcacgcgc
     7981 tacatggagg tctcggccac cggccagttg ctcggcgagt accgggggag acagcgccag
     8041 gaggccactc ccggcaccga tctcaccctc accatcgacc tcgatctcca gcgcgcctgc
     8101 ggcgaggccc tcgacacgct ccgctgcggc gccatcgtcg ccgccgaccc gcgcaccgga
     8161 gagatcctcg ccatggcctc gtacccggct tttgacgcca acgtcttctc cggcgtcatc
     8221 cccgagtccc tctggaccgc catctcctcc gacaccctcc acccgctgct caaccgcccc
     8281 ctcgccgggc tctatccgcc cggttcgacc gccaaggtgg tcacggtcgg cgccggcctc
     8341 gaggagggcc tcatcacgcc ctcgtcgacg ttcaggtcgt gcatcggggg ctaccagttc
     8401 ggcaaccggt tcttccgctg ttgggagaaa gggggccacg gcagccttgt ggccgcccac
     8461 gccatcgagc agtcctgcga tgtctacatg taccaggcgg gactcaagct cggcgtcgac
     8521 cggctgagcg agtactacat taagtgcggc ttcggccgcc cgaccggcat caatctcccc
     8581 ggcgagctga aaggtttcat cccgaccacc gagtggtaca accggaccta cggcaagagc
     8641 ggctggacgc gcgcggttgt gctcaacaac tccatcggcc agggcgaggt cctcgccacg
     8701 ccgctccacc tcctccagct ctactgcggc ctggccggca acggcatcgt gcagcagctc
     8761 cacatcgtgc gcagattcac caccccggcg ggggatgaac tggaggtgag tccgaagatc
     8821 aagttcgccc tccccttctc cgaatctacg ctggcgattc tccgcgaggg catcgagctg
     8881 gtcgtgcagg gagaccgcgg cacggcccgc tcgctgcggc gcaaggaata ccgcattggc
     8941 ggcaaaaccg gcacggccca gaacccgcac ggcaacgacc acgcctggtt ctgcggcgtc
     9001 gccccgctcg aggcccccga gatcgtcgtc gtcgctatcg tcgagaacgc cggccacggt
     9061 tccacggcgg ccgccccggt ggtcggcaag atcatcgacg cctacatgaa gaagaagcgc
     9121 gatcccgagg ccttcttcgc cgaacagcag gcccgcgcgg acagcctggc cgcggcgaga
     9181 gccctcgcgg gcctttccga cacggcggcc gcgccggcgc gcgccgcgag ggggaactga
     9241 tgctcgacta tcgccagctg gactggaaac tcatcggcgc cgtcttcgcg ctctcggcca
     9301 tcggggtggt tctcatcatg accgcccagc accatgccga gagcgagtac gcccgcacct
     9361 tctacctgcg ccagttgctc tggctggtca tcgccctggc cgcggccgcg gccgtgctcc
     9421 acctgcccta ccggctgttc gacttcgccg cctacatttt ctacgccgtc gccgtgctcc
     9481 tgctggtggc ggtgctgatg ttcggcgcca cgcggatggg ggcgacgcgc tggttttctt
     9541 tcggccccat aaatttcgcg ccggcggatg tcgcaaaact cgcactcatt ttcgccctcg
     9601 cccgcttctt cgcctactcg aaaagccccc tcctgtcggg tcagcgggtg gcggtctcgc
     9661 tcggactcac cgtcgtcccc atgatgctga tcctgaagca gcccgacctc ggcaccgccc
     9721 tcactttccc cgtggtcatg tacgcactct ggttctggtc gggcattccc cctctctacc
     9781 tgctgctcat cgcctcgccc ctgttgtcgc tggtagcggc cagcaacgcg atctcgtggt
     9841 cgatctacat ggccgtgctt ctggccgtgc tcatcctgtt caagccgggc cgccagctca
     9901 tcttcagcgt cctcatcgtc gtcgccaacc tcgccttcgg cgccgtcatg ccgctcgtgt
     9961 ggaaccgtct cgaggagtac cagcaactgc gcatcatctc gttcgtcgat ccctcgcagg
    10021 atccgcgcgg cgccggctat cagatcattc agtcgaaaat cgccatcggg tcgggcggtc
    10081 tctggggcaa agggtttctc ggcgggtcgc agaccaagct tgatttcctc cccgagcggc
    10141 acaccgattt cattttcagc gtgctcggcg aggagttcgg cctctggggg gcgctcattg
    10201 tcctcgccct cttcttcgtc ttcttctaca gggggattcg catcgccgcc cgttcccgct
    10261 cgcgttttct ctcctatgtc tctttcgggg gcgtggcgat catcttcttc cagttcctcg
    10321 tcaacatcgg catgacgctc ggtctgatgc ccgtcaccgg cgtgccgctc ccgtttctct
    10381 cctatggcgg cacgtcgctc gtcctctcgt gggtgctggt cgcgttcctc ctcaaagccg
    10441 actaccactg gcaggaatac tgatccgccg gggccgcgct cacagctccc cgctcagcca
    10501 gtgcaggacg ttcagtagaa accgggcatt ctgcggcgcc gccgggctgt tcatcccggc
    10561 cggcgtccgg ttcggacccg ccagttgcgc cgagaacatc gccgcctccc cgaacacggc
    10621 cacccgcccc gcgccgatca ctttcaccgc tccctgacac cacccgctca ccgcgaccgc
    10681 ggctgtgttg gcgtcgaatt gccaggcagt atcgggcgca agcgacacca ctccatcgcc
    10741 gaacaccatc agcccggccg cgctatccgc acgaaaagcc tcgcccgtaa acgacgcgac
    10801 cgagtcgatc cgttccctat cgttgcgccc gcgcgtgatc gcgtggtcgc gcagcgtgcc
    10861 gtccgcccgg cggaagacat acgtgccgcc ggtcggcgag gccgtgtcga cggcgaaacc
    10921 gttgtgcaaa tggaagccga aggccgcggc gagttcggcc gccgccccgg gaaacggcat
    10981 gtgatcggcg atcaacaaga gcgcgccgcc cccctgcacc caggccgcga ccgcggcgat
    11041 ttcgtcgggc gtgaacgccg agggcgtcgg cagcgaccac tcctgcgcgt tgcgctcatg
    11101 gagcgcgttg gagaccacca ggatctcccc ccgcgcgagc gaatcccccg tgaaccggca
    11161 acccgagggg agcacgcggt acccgtcgcg gcgcagcagc tctgcgaacg tgtagtagcg
    11221 gccgccggcg gtgtggaagt tgtgatgccc ctcgtcgatc agcaccaccg ggccgttcgg
    11281 ggcggggaag gccgggtttt cgatcggcgg ccggaaagcc gagtccggca cctgctgggc
    11341 cggggcggcc ggcgccgcca gcattaacag cgacagaacc atcgggcaat acgatcgcac
    11401 gcaagcctcc ttccacggcc gctgccgttt ccgcacgatg atgccaagag acgcggggct
    11461 gcagaatcta cgaatttcct tggccggtgt tgcggccgcg gctatcatgg gccatgcgca
    11521 gacattcgca agagagaacc gggcgggcgc tggcgattct ggcgttcggg gcgctgtgta
    11581 tcagtttcgc cgcggttttt gtcacattgc tcggggctgg ccggctgggg ccgggcgcca
    11641 tcgctttctg gcgaaccttc ttcggcgccg gcatcctctt tgtctgggcg gctctcgccg
    11701 gccaccgcct cggcctgccc tgggcgctga aacgctggac tatcctcgcc ggcgtcctct
    11761 tcgccggcga cctcttcgtc tggcaccgct ccgttctcct ggtcggcgtc ggcatgtcga
    11821 ccattttcgc caacacgcag gtgttcggca ccgccgtcct gagctacttc atcttcaggg
    11881 agcggctcac gctcggattc ttcgccgccg cggccggtgc gattctcggc ctgatcctgc
    11941 tgatcggggt cgggagcgac atcgccttca ccgcccccta cctccgcgga gtgctgctcg
    12001 gcctgctcac ggccgtgttc tatgcccact acatcgtcac gatcaagatt gccgggcagc
    12061 gggagggacc gccgccgagt ttcatcacgc tcatggcctg ggtgtcgtcc gccgcggcgt
    12121 tcttcctgct gatcgcctcg ttgctgtatg agcggcacga attcttcccg cccgactgga
    12181 cgagcgtggg catcctcgtc ctgctcgccc tcgtcgcgca ggcgctcggc tggtgggcga
    12241 tctcgcaggc gctcccgcgt ctcgatgccc accggagcgg cctcgccctg ctgctgcagc
    12301 cggtgctggc cgccgtctgg ggggcgctct ttttcgctga acgcctgacc ccgctgcaga
    12361 cgctcggagc agccataacc ctgctcggaa tctacctcgg aaccgtccgc cgccccgccg
    12421 ggcagcaatc ccgcaccacc ccggcgggca cgtgaacgac tcgtgaggca ttgccaatgg
    12481 aaagcaaccc ccgtaccggc tccctgtcgg aaaagaccgc ggcctggctg aaacacatcg
    12541 aacaggtgaa tcgccacccc atgaccctga atcggggatc ctccgccctc ctcgtggtcg
    12601 acatgcagaa cttcttcctc gacccgaact ccgccgcctt cacctgcggc ggtccgccga
    12661 tcattcccaa tgtaattctg ctgatcgctg ccttccgggc gcagaaccgt cccgtcatct
    12721 acacaaagca cgttcaccac ccgtccggtc acgacgccgg catcatggcc tggtggtgga
    12781 aaggaatgtg cctcgaggga accccggaga gcgaaattca ccccgacctc gccccggcgg
    12841 cgggggagaa ggtcattgcg aaacaccgct actcggcttt ctacggcacc gacctcgaga
    12901 tcgtcctgcg cggcctgcgc atcaccgacc ttgtcatcac cggcgtgatg accaacctct
    12961 gctgcgaatc caccgcccgc gacgcctact tccgcgacta ccgcgtgttc tttccggccg
    13021 atgccgccgg gtcggtcagc gaggaactgc atctggccgc cctcatgggc ctggcgttcg
    13081 ggttcgcgca cgtaacgcgg accgccgacc tcctccggga tctcactgaa tcccgccccg
    13141 cccggactga gccttgatcc aaagctgatg cgccgcacaa aaaaagcacg gcggatcacc
    13201 tgatccgccg cgctgttata aacgtcgctc gtgggctccc gcccgctaca cgagcacgcg
    13261 ctccaaaccg gccgcgataa agtcggcgtc cttcttcgtg atgatgagcg gcggcgagat
    13321 ccggatcgtg tgcccgtggc tgtcgttggc catcaggcgc gctccgagca tctcccggca
    13381 gtacttcatg gcgtcgccgt tcttgacctc gatgccgata aacagcccgc gggcccgcac
    13441 ctctttcacg tgcggcgagc gcgccccgat cgcccggatc tgctcggcga ggtaagcgcc
    13501 gacctgatcg gcgttctccg gcagccgctc ctcgacaatc acgtcgagcg ccgcattccc
    13561 ggccacgcac gcgagcgggt agccgccgta ggtcgacccg tcccggcccg gctggaaaac
    13621 gatgtccatg agtttcgcgt tggtgacgaa acacgacagg ggcaccagcc cgcccgagag
    13681 cgccttgccc aggatgatgc cgtccggaac caccccttcg tgctcgaacg caaacatctt
    13741 gccggtccgc ccgagtccca cctggatctc gtcgaacagg aggatgaggt tgtggcggtc
    13801 ggagagctcg cgcagccctt tgaggaaccc ggccggcgcc tggtacatcc cgccctcccc
    13861 ctgcatcggc tcgacgagga tcccgcaggt gttcttggta atggctttct ccaccttgtc
    13921 gaggctgccg aaatcaaccg tccggaaccc cggcgtgagc gggccgaatc cctgtttgta
    13981 gtaggcgttg ctggagaagg acaccgtggt gatcatgcgg ccgtggaaat tgttgttgaa
    14041 gaccacgatc tcctgctgcc cgtccggaat ccccttctcc ttgtagccgt agtaccgggc
    14101 cagcttgacg gccgtctcca ccgattcgac cccgccgttt ttcggcagca ccttgttgcc
    14161 gttgtcgccg aaacgcgggc ccatctgcgg caccagcgtc gccagcttct tgaggaacac
    14221 gcccagggga tcggtataga cgacgttgga aatgaccgag ccgtaaccgc tctggagcgc
    14281 ctcaacgacc gcctgaacaa tacgcggatg gtggtggccc tggttcacgg ccgagtaggc
    14341 cgccaggcaa tcgaggtacc ggtcgccctt ctcatcgtgc aaccagatcc cctcggcctt
    14401 ccggaccacc agatgctgac gactgtaatg gtgtgcaccg taactgctct cgaaatcgta
    14461 gatcatctgg tcagagtacg tcgagtaccc aaccggtccg cggtgagtcg ttcttacatc
    14521 agccatggta cacctcgtaa aaaagccata aaaaatggcg ttacacgtcg cccaaatata
    14581 tgtcgtccgc cccaaaaact caagtgatgc cgctcgaaaa acgcgcattc tgcgttgtag
    14641 ttcagtcact ttcgcgcctg tttccggccc gctccgcagc gtgatttcat tcggggaatc
    14701 gtcgaaaata gaccagatcg gggctatttg gtccctccgt cgcgcgtcgg accacccgtt
    14761 ccgggtcggt cgggaaggac atgtgcccca agcgaatgtg cgcgaaaaaa aatacccgat
    14821 cgtaccggtt ttgctcgacc taacaggagt aaaattgacc ggataagctt gatttttcgg
    14881 ccccaattgg tatattcttc ggctgattct accaccactt tggaggtctg atgcctaaga
    14941 ttgtcgacaa tgttgaactc ggcccgatga tctggaaaat gcgcgtggct gtcccgcgcc
    15001 tcgtcgcgaa agccaaagcc ggccaattcg tcatcctgcg cgtcaacaac gagggggagc
    15061 gcgtgccgat gtcgatcgcc ggcctcgacc aagccaacgg actcctcacc atcgtctacc
    15121 aggtcgtggg gaagacctcg gcgttgatga cgaccgttgc gccgggccac gagctgtccg
    15181 attgcgtcgg gccgctcggc atccccaccc acgtcgatac ctggggcacc gcctgcgtcg
    15241 tcgggggcgg catcggcatc gcgccggtct acccgatcgc ccaggccttc aaaaaagccg
    15301 gcaacaaagt ggtcacgatc atcggcgccc gcaccaggga cctgcttttt tacgaagagg
    15361 agcacaaggc cgtggccgat gagttctacc cgtgcacgga cgacggatcc tatggccacc
    15421 atggttttgt ctccgacgtt ctcaagcagc ttctcgaccg cggcgccccg atcaagcaca
    15481 tcatggcgat cggcccggtg ccgatgatgc gcgtcgtgtc aaagctgacg gcgccctacg
    15541 gcgtctcgac ctgggtgtcg ctgaacccgc tgatgatcga cggcacgggc atgtgcggcg
    15601 ggtgccgcgt gaaaatcggc gaggaaacca agtttgcctg cgtcgatggt ccggatttcg
    15661 acggccacct ggtcgatttc gacctgctca cctcccgcct gacggcctac cgcgagcagg
    15721 agcggcgggc gatgaaacgg ctgctcgacg acccgaactg ccgcctgacc aagtccgtcc
    15781 aggccttcgc ctacccggat gagaagtgag gaaacgacca tgagcgaaaa agacaccgcc
    15841 ctggggcagg atgaatcccg ggccgaggcc ggctcctcga atccgatcgc caagaaagac
    15901 cgcatgcgca tcccgcgcca gaagatgccg gaacagcagg cggaggtgcg ggtcggcaat
    15961 ttcaaagagg ttcccttcgg gttcacgatt gacctggcgc tcaccgaggc ccagcgctgt
    16021 ctcgagtgtc ccaaagcccc ctgcatcaag ggctgcccgg tggaggtcga catccccggc
    16081 ttcatccgcc tcatcctgga aaaggacttc gccgccgccg cccgcaagat caaggagacg
    16141 aactccctgc ccgccgtctg cggccgcgtc tgcccgcagg aggagcagtg cgaaatcgcc
    16201 tgcgtgctgc acaagaagtt ccagccggtc gcgatcggcg ccctcgaacg cttcgtcgcc
    16261 gactacgagc gcgagaacca tctcgtccag cttccccgga agcagccgcc gacggggcgc
    16321 aaagccgcgg tcgtcggcgc cggcccggcc ggactcacgg tcgccggcga cctcatcctt
    16381 ctcggccacg aggtcactat tttcgaggct ctccacaagc ccggcggcgt gctcgtctac
    16441 ggcattcccg aattccgcct ccccaaggcg atcgtccagt ccgagtgcga ctacctccgc
    16501 gacctcggcg tcgaattccg caattccacc gtcatcggca aaatggacac catcgacgag
    16561 ctcttcgaga tgggctacga cgcggttttc atcggcactg gcgccggcct gccgaatttc
    16621 atgggcgtcc ccggcgaaaa cctcatcggc atctactccg ccaacgagta cctcacccgg
    16681 tcgaacctca tgcgggcgtt cgaggaggac agctacgaca cgccgatcat ccgcggccgc
    16741 aacgtcgccg tcctcggcgg cggcaacacg gccatggatg ccgtccgcac cgccctccgg
    16801 ctcggcgccg aaaatgccta catcgtctac cgccgcagcg aggccgaaat gcccgcccgc
    16861 aaggaggaaa tccaccacgc gctcgaagag ggcgtgcagt tccacatgct cacgaccccc
    16921 atccggttcc tgggcgatga acggaaccgg gtgcgggcga tggagtgcct ccagatggag
    16981 ctcggcgaac cggacgcctc cggccggcgc cggcccgtcc ccatagaagg ttcgaatttc
    17041 accatggagg tcgatctcgt ggtcgtggcg atcggcaacg gttccaaccc gctgatcccg
    17101 cagaccactc ccggcctcaa gaccaaccgg tggcagaata tcgtcgtcga cgaggagacc
    17161 atgcagacct cccgcgaggg ggtctacgcc ggcggcgaca tcgtcaccgg cggggccacg
    17221 gtcatcctcg ccgccggggc ggggaagaaa gccgcccgcg ccatgcacaa gtacatgatg
    17281 gagaaagtca ggacataagc ccgcggcgga tttctccctc cgggtgatgg tagcaataca
    17341 gcagaggcgg gccgcgcggt ccgccttcgc tgtttcaccc tccgccgccc gtcaaggccg
    17401 ccatgctcac cagcgaactc gtcaaacgcc tcgccgccgc cgccggcttc gacctctgcg
    17461 gcgtcacctc ccccgaggtc atccccgagg cccgcgaccg gtttctccgg tggctcgccg
    17521 agggccgcca cgccgagatg cactggatcg cccgctccac acaccgtcgc gttgacccaa
    17581 gagaactgct gccgagcgtc aagtcggtca tcatgctcgg cgttaactac tacaatccca
    17641 acagccccga tcgcccgccg ggccacggcc gcgtctcccg ctacgcccgg ggccgcgact
    17701 accacaaggt catccggcgc ctgaccctcc acctgatcca acgcctgcag gagcaggttg
    17761 ccccgcagcg ccacgacttc atctggtacg tcgactacgg cccctttctc gaacgcgcct
    17821 atgcggccag ggccgggctc ggcttcatcg gcaagaacag catgctcatc aaccggacgt
    17881 ttggatcgtg ggtcttcctc tccgagatcc tcacatcgct ggcgcttgat ccggacgaac
    17941 cctgcggcgg gcgccacggc gcctgcgggg agtgcaccgc ctgcattgat gcctgcccga
    18001 ccggggccat cgtcggcccg gccatgatcg aggccgcgaa gtgcatctcg tacctgacca
    18061 tcgaacgacc ccgcgcgatt cccgaagcgc tgcagagccg catgggggcg ctcattttcg
    18121 gctgcgacat ttgccagcag gtctgcccgt acaatcggcg ggcgacgccg accccgcacc
    18181 gggaactcct gccggccgcc ggcgcggggg agttcgtcga cacccggcgc gtcctcgccc
    18241 tgcgcgaccg cgaggagttc ctcgcgttcg cggccggcac gccgctcgtt cggccgcgcc
    18301 tggagggcct gcagcgcaac gcccggatcg tcctggagaa cgagagccgc cgcacccggg
    18361 aaggcggcgg cgccgccggc gggggagagg ttccgcaata aacggttgcc ccgtccgggg
    18421 gatgtgccta ttattgccgt atggtcaagg acgcgcaagc gacagcgatc cgaacgggct
    18481 ccggccgggg tccggggcgg atctccgtgc gccgctgctc ccccgggcct tcggccccgg
    18541 tcgcgctctc cgtccctccc gcgtcgcgcc gcgcccgcac gagcaggaaa atcccagttc
    18601 caatagcagt gaggtggcaa caacggtgaa gccgggagcc ttttccatta gtgccggtcg
    18661 gacggaggcg gcctgtcagc gcgccctgcg ccggctggaa cgcaacgcgg ttctgccgcg
    18721 catcatggtg cgcgacgccg ggctgttttc gcggcagccg gccgtccgcg acaagatcgc
    18781 caaccgcctc ggttgggtcg atgtggccgg caccatccgc aaacgcgtcg ccgccatcga
    18841 atcgttcggt cacgacgtca tcaaggcggg cttccgcgac gtcgtgctcc tcggcatggg
    18901 cggatcctcg ctctgccccg atctcttcgg ccgcattttc ggccgccacc gatcgctcga
    18961 ctcttttgcc gtgctcgatt ccaccgatcc ccgcgccgtc accgccgctc tccgcaaggt
    19021 ccagcttgac cgcacgctct tcatcgtctc ctccaagtcg ggcgccacgg tcgagacgcg
    19081 ctcgcagatg atctgcttcc tcgaccgtct gcggcaggag ggtttttcgc ggcaggggcg
    19141 ccagttcgtc gccatcaccg actcggacag cgcgctgcac tccgccgccc ggagcgaggg
    19201 attccgccgc atctacctga acccgcccga catcgggggg cgctactcgg ccctctccta
    19261 cttcgggctc gtccccggtt tcttcgccgg ggtcaacctg gcggcgctgc tggacgatgc
    19321 gtatgcgatg gagaagatcc tccgcgcgcg cgacgacgag accaacccgg ccgcagcgct
    19381 ggccgcgctc atggcggcgg gcgccgagtc gggcagcgac aagatgacat tcatcgcctc
    19441 gcggggtctc gccccgcttg tcccgtggat cgagcaactg gtggccgagt cgaccgggaa
    19501 gaagaccagg ggtgtagtcc cgatcgacgg cgagccgctc ggcgcagcgg cggactaccg
    19561 ggacgatcgg atgttcgtca ccctccgcct ggcctccgaa aaacctccgc tgcccgagaa
    19621 ccttcgcgcc gctttccgga agaaagggtt cccgatcgtc gacctcagtt tgccctcgta
    19681 cgatgccctg ggcggccagt ttctcctctg ggaggtcgcc gtggccgcgg ccggctatct
    19741 catggggatc aacccgttcg atgaacccaa cgtcaccgag agcaaacgca acaccgaacg
    19801 catcctggac caatatcgcg agtcgggcca gttcgccgac ctggccgcgg gccggaccta
    19861 cggccgtctc acgctcgtga gccacggggg cgagagtcac ctccgcccgt cggacctggc
    19921 cgatttgaaa cggctgctca cccgattcct ggccggcctc cggccgcccg aatatgtcgc
    19981 gctgctctgc tacagcaagg aagagcgcgc tctggatcgc cagttcgcgg gcatgcgggc
    20041 ggccatccgg cgccgccggg gggtcgcgac cctgtgcggc tacggaccgc gctacctcca
    20101 ttccatcggc cagctctaca agggcgggcc cgatgtcggc cggttcatcg tcttcctccg
    20161 ggccgattat gaccggctcg agattcccgg gcggttctac gatttcgggc agctcatcac
    20221 ggcccaggcg atcggcgatg tgcgcgccct ggcgagtcgc gacctccccg tgctcgtctt
    20281 cgccgtcaag ggaaacgtca ccactgcggt aaaggagttc gccgatgtcc tccatgcggt
    20341 ttgccaatga acaggatacc cgagtgaaag atttcgatcc ctggcgcggc ttcacctcca
    20401 gcgctctctc gcccgagtgg cggaagaaac tcagcgagac ggcccgccgt tgccgcgcgc
    20461 agattctcaa gatgaccagc ctcgccgcct ccggccatcc cggcggttcc atgtcgtcgc
    20521 tcgagctgta cctcactctc taccacatgg cccgcgtcga tccccgcaac ccgatgcgcg
    20581 atgaccgcga ccgcatcatc gtgtcgcacg gccacacctc gccgggcgct tatacggcgc
    20641 tggcggccgc cggtttcttc gatcccgccg cggccatgca cggtttccgc caggccggct
    20701 cccccttcga agggcatgtc gagcggagcg tccccggcat cgaatgggac accggcaacc
    20761 tcgggcaggg gctctcggtc ggcatcggca aagcgctcta cgcgcggctc tcgggtctga
    20821 atttccacac gttcgtgatc atgggcgacg gcgagcagca gaaggggcag gtgggcgagg
    20881 cgcgccgggt cgccgccaaa ttcgccctct cccgtctgac cgcgatcatc gaccgcaacc
    20941 gcctccagat ttccggccgc accgtcgaca tcctgccgca ggatctcgcc gccgagtggg
    21001 aggccgacgg ttggcaggcg gtcgaaatcg acggccactc atttgaccag atctacgggg
    21061 aactgcaccg cgccgtcacc aatgaaggcc gcccgatcgt catcatcgcc aacaccgtca
    21121 tgggcaaaga cgtcggcttc atggaaaacg acgaggcttt ccacggggct ccggtcgccc
    21181 ccgacatgct caccccggcc ctcgccctcc tgggcgacca cgggaaagac ctccccgcgc
    21241 tgcgcgagct gcgcaagcag gggccgccgc ccgcgttcaa gatcccccgg cccccctacc
    21301 cgccggtcga aaccgggcag ccggccacct acggcgccga cgtcaaaatg gacaaccgca
    21361 cggctttcgg caaagctctc ctggccgtcg ccgatctcaa cctcccccgc gaggacttcg
    21421 tcatggccgt cttcgactgt gacctcgccg gctcggtcaa gactgcggcc ttcggccaga
    21481 agtaccccga caattttttc cagtgcggca ttaccgaaca caacactgcc gccatcgccg
    21541 gctccctctc cgccgagcgc gccgtctcga tctgggccga cttcgcggtc ttcggcgtcg
    21601 acgagacttt caaccaggcc cgcctgaacg atatcaacca cgccaatctg aaactcttct
    21661 gcacccacac cggcgttcag gtcggcgagg acggcaagac ccaccagtgc atcgactact
    21721 tcgccctcct caactcgact ttcggctgga gagtcatcac cccggccgat cccaaccaga
    21781 ccgaccgcat cacccggtgg gttttgagcc ggccgggcaa cttcgccgtg ttcatggggc
    21841 gttccgcgac gccgatcatc cccgacgccg acggccgtcc cttcttcggc ggcgactacg
    21901 agtaccgcta cgggcgcatg gacgtcctcc ggcgcggcga gcgcctcgcc ctcgtcgccg
    21961 ccggcaacat gtgcgcaatc gctctcgacg cctggcagcg gctgtcccga gagggcgtgg
    22021 gaatctcgct catcagcgtg gccgactggt cggactttca cgccgatgat ctccagatgc
    22081 tggcggccca cgagcacctt gtcgtgctcg aggaccacaa cgtcaagacc ggattgggga
    22141 ccgctcttgc cgccgagctc ttccaggccg gcgcccgctc cacgctcacc aaaatgggcg
    22201 tgacggcgta cgcctcctcg ggcccgcccg ccgacctctt ccggcttttg gggctcgacc
    22261 cggcgtccgt ggtcgctcgg gtgaaggcca ttctcggctg acggtcctcg gcagtatccg
    22321 cgccgtcggg ccgcaggcgt ccgccggccc gggcggggct gtgcctcccg ccgccgggcg
    22381 tttccgccgg caagttttcc cttgcccgcc ctccccgcgc gcggttatgc taccgccgtg
    22441 aacgccaccg ttccccgaac ctgctgtcca caattgcgga gcctgtgagc gccatgtttg
    22501 tcgcgtccgt cagccggcgt actgtccgcc ttgccgccgt taccgaggcc caggggcgcg
    22561 tcaccctgaa agaccaggcc cgctttgacg cggcggactt cgacagtttc gaaactattc
    22621 tccagcgcta tttcagccgg cggcgcaccg agggcgcgct cttcttcgtc gccgtggccg
    22681 gcctcccggt cggctcgacc gtctcctcgg ccctgctgcc atgggcgatc gacggcgaca
    22741 gcattcgcgc gcacttctca tttgacgtcg tccggctcgt gaacgaacac ctcgccaccg
    22801 cgcgcggtct gttcgacctg ccgcccgatg cgctcttcac gatcaacgac caaccggcgg
    22861 ttcccggcaa catcggtctc atggcggtcg atgaacttct cgccgaggtg ctcatggtgc
    22921 gccgggacaa cggctacctc agcttcctca ccaacggcgg ccacaccggt ctcgcccccg
    22981 ccacccagct cgaggccgag ttgtggcagt atctctacgc ccagcgggat gtggtcgagg
    23041 tcggcgacgt ggtcagttcg gcggggcttg tccggctcgt ggacttctat ctcgagtcgc
    23101 acggccacga agtgccggag tggctgcaga acgccccgga cccgccctcc cgcatcgtcg
    23161 agacggccct cgcgggaaaa cagcaggtgg ctgtcgacgc cgtcgacctg ttcgtcgact
    23221 gcttcgcggg cgaagcggcc aacctcgccc tgcgcggcgc gactacgggc gggctctacc
    23281 tcgccggccg cgtcaccacc gagctgctcc cctcgctcga ccagggccgc tttctggaca
    23341 actacctcaa gcgcggggcc gccgaggaga agctgcgtcc cattcccatc cgggtgattc
    23401 tggacagcgc cgcgccgctg cggggagtcg cccgcatggc tctggaattc gagcacccgc
    23461 aatgacgcgc gccgttgcgt tcctccacgg cgtctaccgc cgccgcgatc ttccgttcta
    23521 ccgcgcgctc tgccgcgggc ggctgacgat tgcggtcgac ggcggttacc gctttttccg
    23581 gatggctggg ctgtttcccg acattctgct gggcgatttt gactcgctga aacgcctccc
    23641 cgccaacctc ccgccccgca cccgcgtcct gcgattcccg tcccgcaagg acaagaccga
    23701 cgcccacctg gcgattgagt gctgtcggga gcgcggcctc ggggcgatcg acctggtgat
    23761 gcccggcctc ggcgagattg accatgccct cggcgtcgtc tttctcctcg gcctgcgcga
    23821 tcgccccgcc gcccgaagcg gccgcgccga tatccgtatg gtcagcccgc aatgcgagat
    23881 ccgcctcgtg gccgacagcc ggatcaccta tcgccggcgc gggggagacc tcgtctcggt
    23941 tctgccgctc tcggcgcgaa tccggctcac caccgagggg accgagtacg acgtgactga
    24001 ggcttggctg caattcggcg atacccgggg gctgcgcaat cgcatcgtcc gcgcccgcgc
    24061 gacggtcgct gtccgggggc aagcgctggt ggtgcgccgt ttttccggcg gcttggcggc
    24121 ccgcccataa ggcggtgtct cccatcggga aaactgctgt cgcggccgcc cagccgcccg
    24181 gcgcgcgccg gcccggcgct cccgactctc ccccgcaaaa ttgccccccg tttatcttga
    24241 caacagggtg cagatgccta tattcagggc ctgtaaaaat cggccgggca tccagagatc
    24301 gttgtgtcct gccgacaaga ccgtagacac tgggcgatcg tccaatggta ggacatgcgg
    24361 ctctggaccg tagaatcggg gttcgaatcc ctgtcgccca gcttttttat gcccgccgcg
    24421 cccggcgctc tcagccgaac gtcgccaaca gcacccctgc cgccaccgcc gacccgataa
    24481 ctcccgccac attcggcgcc atggcatgca tgatgaggta gttgttggga tcctcgcggg
    24541 cgcccatcac ctggcagacg cgcgccgaat gcggcaccgc cgagacgccc gcggcgccga
    24601 tgatcgggtt gattttgttc cgcgcgatca ggttcatcac tttcgcgaac agcacgccgc
    24661 cggccgtcgc gatcgcaaac gaggccgccc cgagcacgaa caccttgatc gagtccggcg
    24721 tgagaaaccg gctcgcatcg gtcgaggcgc caacggtgag gccgagcagg atcgtcacga
    24781 tgtcgataaa cgccgtccgc gccgtcgccg ccagccgctc cgtgaccccg gactccttga
    24841 gcagattccc gaaaaacagc atgcccagca gaatcagcgc gcccggggcg atgaaacagg
    24901 tgatgatgaa ggcgacaatg gggaagagga tgcgcagccg cttgctcacc ggcggcgccg
    24961 gtttcatccg gatcaggcgc tcgttgcgcg tcgtcagcag cttgatgatc ggcggctgaa
    25021 tcaccggcac cagcgccatg taggagtagg ccgaaaccgc gatcgccccg agcaggtgcg
    25081 gcgccagctt cgacgtcaga aagatcgccg tcggtccgtc cgccccgccg atgatgccga
    25141 tcgaggcgga actgtcggcg tcaaagccga gccagagcgc ccccaggaaa gtcacgaaga
    25201 tgcccaactg ggccgccgcg ccgagcagaa tcagccgcgg actggcgagc atggccgaga
    25261 agtcggtcat cgccccgatc cccagaaata tcagcggcgg gtaccacccc tcgctcactc
    25321 ccttgtagag gatcgacagc acgctccctt cctcgtaaat gccgatctcc tggccgggcg
    25381 cgagggggat gttccccatc agaattccga agccgattgg cacgagcagg agcggctcgt
    25441 accctttggt gagggccagg aagaggaagc cgcacccggc gaggagcatt acgaggttgc
    25501 cccaactgaa gttgaagaac gcgccctgct ggaggaaatt gagcagcatg tccatagcgt
    25561 gtcccgtggc ggggccgcgc ccgctactcg aactccacga gtgtctggtt ttcgcgcacc
    25621 gagtcgccga ccgccaccgg gatcgccttc accttgcccg ccgtcggcgc cgcgatcgcg
    25681 gtcttcattt tcatcgcctc gatcgacagg aggatgtccc cctgtttgac cgccattccg
    25741 actttcgcct tgatctcgac caccgtcccg gcgatcggcg aggcgaccgc tccctggccg
    25801 gcggcggccg gcgccgccgg ggtcggggcg ggggcaggcg gcccgcccgc cgtcagcgac
    25861 tggcgcggcc gcgggcgcgg cgccgcggcc gggaaaccct ccccggcgtc aagcacctcg
    25921 acatcaactt catacgccac cccgtgcacc gttattctca gcttcatcgt ctcactcctg
    25981 gtctctcgtt tctttctctg gggcgctacc ttcgccgcgg cacgacgtgc gatcccatca
    26041 gcaccgcccg gcccgaggcc gaccacgtgc gcgtcccgcc atcgaccggc aacagccgcc
    26101 ggatgcgggt gatgtggaac cgcccctggg tgacggtggc cgcggccgcc gcgatcagca
    26161 ccagcgtggt ggtgtcgatc gtcggctcct tcccgaaagc cgcctcgtcg gcctgcgcct
    26221 cgcgcgcccg ccaccgcttg tcgagccggc cgaacaggga gacggccgtg acgatgatcg
    26281 ccagcgcggc aaacacgacc agcaggccga ccacgacaaa aagcgcgaca ttgaactctg
    26341 ctccgtccgc catcaccgtc tcccgggcta gagcgggatc agcccgtgtt tcttggccgg
    26401 ccgcagctcc cgcttggctt tcagcacttc gagcgcctgg gctaggtagg ggcgggtgtc
    26461 ggccggttcc agtatctcat cgacgagtcc gtgcgccgcg gcggcgtacg ggttcgcgaa
    26521 catgtccttg tactccgtga tccgccgcgc ccgctcctcc tcggggtcgg cggcctcgcc
    26581 gatttctttg cggtagagaa cattgaccgc tccctcggcg cccatgacgg cgatttcggc
    26641 cgagggccag gcgcagagca ggtcggcgcc catgctcttg gcgcacatgg cgaggtaggc
    26701 gccgccgtag gccttgcgga tcaccactgt catcttcggc acggtcgcgg cggcgtaggc
    26761 gaagagcatc ttcgcgccgt ggcggataat cccgctgaat tcctgctgca cccccggcat
    26821 gaaccccggc acgtccacga gcgtgagaat ggggatattg aaggcgttgc agaaccggat
    26881 gaaccgcgcc gccttgtccg aggaatcgat atcgagcacc cccgctttct gcacgggctg
    26941 gttcgccacc accccgatcg aataccccgc cagccgcccg aacccgatca gaatgttcgg
    27001 ggcaaagtgc tccatcactt cgaggaagtc cccctggtcg aggattccgg cgatcacctc
    27061 ccgcatgtcg tacggttccc ggggatcctc cggcacgatt tcgttcatcg ccggatcggg
    27121 gccgatcgcc tcctcgtgca gcgccgcgtc gaacggcggg tcctcggtgt tgttgctcgg
    27181 caggtaggac agcagccgcc gcgcgagggc gagcgcgtgt tcgtcgtttt ccgcaatgaa
    27241 atgcaccacc cccgagtagt aggcgtggct ctccgcgccg ccgagctgtt cggccgtgat
    27301 ctcctcgccc gtcacctgct tgatcacgag cgggccggtg atatagagct gtccctggtg
    27361 gcgcacctgg atgataaaat cggtcagcgc gggggagtag gccgcgccgc ccgcgcacgg
    27421 accggcgatt atgctgatct gcggcaccac cccggacaag agcacgttgc ggtagaaaat
    27481 gtgcccgtat ccctggagcg agtccacccc ctcctgaatc cgcgcgccgc cggagtcgtt
    27541 gatcatgacc aggggatccc cggttttgag cgccgcgtcc atggcttcgc tgatcttccg
    27601 tccggtcatt tcacccaccg acccgccccc gaccgtgaaa tcctggctcg cgacgtacac
    27661 cgggcggccg tcgatgatcc cgtgcccgca caccacccct tcgcccggaa attccttccc
    27721 cgccatcccg aacgccgtct gccggtgctg gatgaacaga agcgactcgc ggaatgtccc
    27781 tggctcaaac aacaggtcga tgcgctcgcg ggcggtcagc ttgccgcgct cgtgctgttt
    27841 gtcgactgcc tggggaccgc cccccgcggt catttggccg cgtttccggc gcagggcctc
    27901 gatgcgtgga tcagccatgt ccaatccttg ttacaaattt cacatacttc gccccggtag
    27961 tgtaagtgct ttcctctaaa atgcaagcga aagtttgccc ccgctccgct tttggcgcgg
    28021 ccgcgcacag ggcgcccgcc ggggggcggc ggaggccggt cgaccgcgct tctcctgccg
    28081 ccggctgtca cccctttccc gcaaaaaagg agaccgccgg cggatacccg acggcggccc
    28141 gaatgtccga tgtcgcgtgc gcgcggctag tctttcgcgg cgagtttcgc ccacgtgtcc
    28201 cggaggccca cggtcctgtt gaaaatcggc ctcccgggcg cggagaactt gttgtccacg
    28261 cagaaatagc cgtggcgcag aaactggaag cggtcgcccg gacgggcctc tttcagcatc
    28321 ggttcgagtc tgcagccggt caggatgcgc agcgaatgcg ggttgatgtg ggcggtgaaa
    28381 tccttcccgg cggccgcctc ctccggcgca atctcctccg gaaactcgtt cgagaacagg
    28441 ttctcgtacc accgcacctc gccttcgaac gcgtgcgcgg ccgacaccca gtggagcgtc
    28501 cccttcacct tgcggccgtc cggcgcctcg cccccccgcg aggccgggtc gtacgtgcag
    28561 cggagctcca caatcttccc ggccgcatcc ttgaccgcct cctggcaccg gatgaagtag
    28621 gcgtgcttca ggcgcacttc ggcgccgggg gagaggcgga aaaacttcgc gggcgggtgc
    28681 tccatgaagt catcgcgctc gatatagatt tcccgggcga acggcaccat gcgggttccc
    28741 tcggccgcat tttccggatt gttgaccgcc tcgatctcct cgacccggtc ctccgggtag
    28801 ttcgtgatca cgaccttgag cggatcaagc accgccatca cccgccgcgc ccgcttgttg
    28861 aggtcgtcgc gcaggcagtg ctccagaagc gcgaggtcgg ccaccgagcc tttcttcttg
    28921 gccacgccca cccgctcggc gaaatcgcgc agcgactccg gcgtgtaccc ccgccgccgc
    28981 aggccggaaa tcgtgggcat ccgcggatcg tcccacccgt cgacatactt ctcctgcacc
    29041 agccgcagca gcttccgctt gctcatcacc gtgtaggtga gatcgaggcg ggcgaactcg
    29101 atctgccgcg ggtggtagat ccccagctgg tcgagaaacc agttgtacag cgggcggtgg
    29161 tcctcgaact cgagcgtgca gatcgagtgc gtgatcccct cgaccgagtc cgactgaccg
    29221 tgcgcccagt cgtacatggg gtagatgcac caggcgtcgc cggtgcggtg gtgccgggcg
    29281 tgcagcaccc ggtacatgac cgggtcgcgc aggttgacat tccccgctcc catgtcgatc
    29341 ttcgcccgca gcgtcttcgc cccgttggga aactccccgt tcttcatccg ctcgaacaga
    29401 tccagattct cctcgatcgg ccggttgcgg aagggcgact cctggcccgg ctcggtcagc
    29461 gtcccccgcg tcgcgcggat ctcctcgggc gtcagatcat cgacataggc tttccccgcc
    29521 ttgatcaact gcaccgccca ctcgtagagc tgcgggaaat agtccgaggc gaaaaagaga
    29581 cggtcctccc agtcggctcc gagccaccgg acatcctcga tgatggagtt gacgtactcc
    29641 tcctcctctt tagtcggatt cgtgtcgtcg aagcggaggt tgaacttccc gccgtaggtc
    29701 tgggccaggc cgtagttcag gagaatcgac ttggcgtgcc cgatatggag gtacccgttc
    29761 ggctccggag gaaagcgggt gtgcacccgt ccgtcgtagg ttcccttcgc aaggtcatca
    29821 tcgataatgt cgcgaatgaa gttcgatgtc gctcgttcgt cgctggccat aggcaccctt
    29881 catcaatctt ctcgtatcac tctttatcgg cggcgcgcag ggtcgggctg aacgcctccg
    29941 ccgcccaatt ccacacacta atacaccctt tccgcggagt caacgcaatt tccgcctggc
    30001 cccggacgtt ggccacgttg cggttcggcg gccccatccc cgaacctccc gccgtttctg
    30061 tccggcatac tgcgccggcg acggtccgat ttccgactat attcaattag tgatggactt
    30121 gctggatcag gctgcgccag aagacatacg gtagccgtaa acctgctggg ctcgatcatc
    30181 caggggcgcc gccgctgccg gttcaggatt caccttcgat gacgctgcta tcgcatgccc
    30241 ggccaggcca tctgtctcgc tctcgggcag actgaatgct gctcctcgtg ctggtctggg
    30301 attacatacc cccggcagga ctcgaacctg ctaccctctg cttagaaggc agatgctcta
    30361 tttttcgggt tgtcccgata aaagagcgtt gcggcacaac gacttacggc gctacgtttg
    30421 cctatgagcc gtgaaaaatc agaggcccta ttattcgggg cttgaggggt gattttgacc
    30481 aaagggcctc tgacagtcac ttgcttcatg gcggcaagtg tactcgtttc aatgttctga
    30541 atcaacaaaa aacaacactc ctcttgtcat acgcgtttcg ccgatgcagc ttcttcagcc
    30601 tcctgccgag acaaggtgtc accttaggaa atagaaagtg gcccggcctg tcctccctga
    30661 agcttctgag tcaccacaat caagaagctt ttctcttgcg attgattttt gatgctgata
    30721 catttgtaac aagaatgagc gattcgacat agaattgcag aatatggatt gtgaggagga
    30781 atactcatga ggcaaacaag tcactgcaga ttcagaaatc taccgatcga gtttgacgaa
    30841 atagttgttg aacaaccaca ggaactcgga aacaatagtg gtttaccacc tgtaacggag
    30901 tttgaaaatt gctctgaata cagcgaggcg gactgcgctc gattcggctg ccttctcgat
    30961 ccgacgtgtg gtttgggtgg cgctgcccct ttttcagagg agtaatcagc agcgttcatg
    31021 agtattatca gtacgagacc tgcccgcttt cattgtatcg ggtctccaat cttgggtctt
    31081 attcagttca acacagtgtc ccgatagaat ccaccgtaca atttattctt aggactgaga
    31141 gattttttgt ttgcgcttga gtttttgtat agatatattt gtaataagta cgagtggctc
    31201 cataaag
//
LOCUS       DAOPOE010000028        31163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1892_length_31163_cov_5.041404, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000028 DAOPOE010000000
VERSION     DAOPOE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31163
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_31163_cov_5.041404"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2072
                     /gene="lon"
                     /locus_tag="PKM94_05515"
     CDS             <1..2072
                     /gene="lon"
                     /locus_tag="PKM94_05515"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD66133.1"
                     /translation="VVDEITHLDPSYSPEQVTVLKMNQKDPSLLADRAASIFHFPLPA
                     KQEILESVDLSMRYDRLLASLNSELMRVATAASVNENVRRNMAEEERRVFLRQQLAEI
                     RRQLGEDPSEEREAAALRHQIKSSNSLPPDVVARATIEIDRLSELSPASAEYGVTKNY
                     LNWLLSLPWGKVTPEDYSMAEVARVLSAEHYGPTQLKEQILQRLSVRKLLGGTSEGPT
                     LCLIGAPGTGKASLAKAMAKAMGKRLVRISVGGISEVSEIKGSPRTFLGAMPGKIVRA
                     MRDAGSCDPVVLIEDIDYFNMDNDSSVNMVLLEAIDSRWNKAFLDNYLGVPFDLSKAF
                     FICSVRSFEEIPEQFVPRLDIIELPAYIEKEKIVISKKYIIPKMLRKHGLSKAEVEFE
                     TKALTRIISNYTMEAGLLVFSQQIEKILRKIALEKAEKPRKNWVIRPDTIELYLGQPV
                     FIPEKAETKPEIGCATGLAWTGAGGDLMFIEGLKMKGDGQIITTGSLGDVMRESIQAA
                     HSYVRSKADVLGIDFNDFNEFDIHIHFPSGAIPKDGPSAGITVCLVIASLMAERPIRN
                     EIAMTGEVTLRGRVLPVGGIKEKVSAAYRAGILTVVLPKENEKDLKDLPKEILRRTAF
                     RFIERVDELFELCLLDFTPSAYTLEKIFAEEIEKAKRRSSAGKAAARKVSKKPGGKPS
                     TKRPRTR"
     gene            2441..4786
                     /locus_tag="PKM94_05520"
     CDS             2441..4786
                     /locus_tag="PKM94_05520"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66134.1"
                     /translation="MKSKLLVLMLILLLAGAGPAAAQFQQDPDDAGAADTIDLVYSVV
                     PDHATNQLHLRADLYGFTDANTIFGVTMGFKWINPNLQMDSANSPADIRTAFDLGTFY
                     YENANIALTNTNHRFIFGGLSAFSVGLQPAPNRRLWASYFFTLSSWGECDTVSIDTLF
                     WQPGTEFNLVDDNNNEIIPYWTGRERYKDTSCAEPSNLIVTPDSLFFSAVQGGGAPSG
                     QQFVISSDNEPLAFTVTETSTWMAVSPLAGNTPRSITVLPNIVGLAPATYIDSVMVAA
                     PAAANTPQWVKVVLLVQEPPPTIAATPTAFFFNAVAGGANPDPKTLTIKNTGGQVLNW
                     TLSHSQGWLSLAPMAGTDSAAVTVSVDITGLAFNTYYDTVVITAAGATNTPVRVPVTL
                     SVGSDLPIIAVDSAFNYVVVPWPAREVGPWTITIRNDGAGAMNFWLEENASRILSMSP
                     MSGAAPLDVQVTFKLNAGNAGVDYYDTLWVYSNEAINSPFPVVFLFHYVSDPALMIVT
                     ADTVRLTLYECEMGIRNLPPVGGFIVDNAGGDDPMEFVLGWESELFDISTLSNVAPAT
                     VSVQANFLNLPIGVYYDTIVVTARKSTNPADTVIVKYSVIPPTQQPKIWVSNSSFVVP
                     AQENSGPLPDIGMAIHNEFGGCMPWYIVENVPWLFPSDTSGINPASLIFGVNSDGFLF
                     GEYPDSIKLYAPSASNSPGTIRVRLKVWRFHGDCDYNSVINVLDLTYMISFLFRGGPQ
                     PQPTQLVGDLNCDHLVNIADLTYFIEYLFRNGPIPCWNPYK"
     gene            5014..7242
                     /locus_tag="PKM94_05525"
     CDS             5014..7242
                     /locus_tag="PKM94_05525"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOD66135.1"
                     /translation="MSSASVTRILAVLAALVLLATAAGFGDDDESRDWNTAPASEIHE
                     RLAAAKAGDLQRRTALKQLLGGSEATTQTNYDVTFYDVKLRINDTTEIVWGRVTFVAT
                     AAEGNVGSVEVDLFLNMTIDSIVGSSGQLAFTRLGNVVTIDLDGTYRVGEQFAFDFWY
                     HGHPTESAGFGAFTFGTYGAYTAISTLSEPYFARTWWPCKDRMDDKADSFNIAIEVDT
                     SLYAASNGTLDSMVAHGGNTHTYYYRVRYPMATYLFMLAISEFQVWYDQWVYNGGADT
                     MPLEYAVYPSWYTASLSSFAVTPGALTIFSDVFGPYPFATEKYGHAHFTWGGAMEHQT
                     MTSLTANSFSMSTTVIVHEMAHQWWGDLITCKSWRDIWLNEGWASYAEALYYEALGGW
                     TAYINHMIGMYFTAGGTVYCQDTSSTDAIFTSRVYDKGAWVLHSLRGVLGDSLFFAGV
                     HAYYNSPYQFKAATTEDFKNVFEAATGVELDWFFNEWVYGTYYPNFWWYWMSEPAAGG
                     GWDVYLRVRQVQTTEPRVFTMPIKFTFTGVSDTVTVWADEKIERTKIHLPANPSALSL
                     DPMNWMIKSQQRLAWDMFVITPSDGLDTVRQYSAYTDTIELRGGSGWQTWSVTAGSLP
                     TGLSLSNEGVISGTTSDTGRFAFTVFVDDNSYSYTDQVDFTLYAAPSAGKPGDVTNDG
                     QVNVADLTLLIDFLFRGGEPPVILNSADVNGSCDISVADVTYLVAHLFRGGPAPLPGC
                     VN"
     gene            7398..8720
                     /gene="miaB"
                     /locus_tag="PKM94_05530"
     CDS             7398..8720
                     /gene="miaB"
                     /locus_tag="PKM94_05530"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOD66136.1"
                     /translation="MATTRDIRTFHITTFGCQMNLADSSTLAASLLTRGYRRAASPAE
                     ADLLILNTCSVREKAEQRVFGRLGEISQLKRRKPHVKVAVVGCMAQRLGDELVRRAPQ
                     VDIVLGTDRLFELPDVLEGIEGTPRIMTAFGHEDMDSIVPVKETPFSGFVTISRGCDN
                     YCTYCIVPYVRGRERAHAAGFILDAVRKMADEGVVEVTLLGQNVNSYAWEGIGFPELL
                     LRVARETGVQRVRYMTSHPKDLSRRLVDVMADEPKIMPHIHLPLQSGSDRILRRMGRA
                     YTLEHYLKIIDYIRARLAYAALTTDLIVGFPSETEAEFEQTLAAVRRIEYDAAFMFRY
                     SVRPGTTAARSADDVPEAEKIRRLNTLIALQQQISHARNQREVGQVRSALVEGPSRRD
                     PRVLRARTEGNKTVLFDSGRAKPGDVVAVRIAAADAFTLHGEMVEAGA"
     gene            8717..11644
                     /locus_tag="PKM94_05535"
     CDS             8717..11644
                     /locus_tag="PKM94_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66137.1"
                     /translation="MIEHLPIILLPAAVALTVIFLTVRLRLFADEEAAGRWLFVAGGA
                     VLTAAAVWHGVRQTAAYADWFVAAAYPYLDLAHFAVILAGLLLAAAGLAFYADGWQTR
                     REDIEVRDQKLSILDNLQRDARGPYQLVDLLSMSIREIVSHLPDAAGAVFLLNRKHRQ
                     FVLTASVGLSQEETALLEYFPLQRNTVSQSIDIGEPILGRSFSFIERSGAERPSRFQS
                     VLVLPLVSGMEKIGGILLVAEAEHYFSRAEIRYLQPVAEWIAEKIKSARLERELTAAV
                     RQSEERAQRGDDLATRLRAATAALGARDGVAAFCRALAGVAESRSVHLFGLTGGALQF
                     YGGSESLGDISENYRTALVDALDRNKPLVINQEGVGDGGRKFVAVSSLVAPLGGREAS
                     ALLLRREDGPFRVDDDDLKQLELFGGLARLALQRADTERLDLTRRKGFETVLQLLRFD
                     VDVEPDKQPDFFTAHMAPALPAGSLAVLFRRENDGSFTAVGGAPGAADLRLLPGEGIV
                     GEAANRAEGRFVFSRAAVGRAIEALESGSREQMQALFGEQGVPGFAAACPILRLDRVA
                     AVALFLLYDATESERGEWERLLTLASSLYSVRLTVAELNRRVLEAQTTGGGVDQDLVG
                     RMVNELNNHLSAVIGNAELAFTRTNLTGDVAQHLRSVIAEAEQAAKALKDSLGRMRLP
                     APSTAVAAVEDKSLGAVVKAVLRRLQVAENLYLAGGRAREMAVLAEADGVVPVAEERL
                     YGLVEEMLNRFGAAAADDDVLTLRLYRSDDRLCLDLSRHHRNFPPVDKVAGFGEYEGA
                     PEALRQRPSDTFLRYLADSDVTYAHDRHTQPSTYLSFQFPTAAPGGSARAARPKEAVR
                     ILAIDDQTVILDLIAAMSQSMGYEVAVASSGPEGIALARRQRFTLVLTDLAMPGMSGL
                     EVAAEIRKLQPDTPIVLVTGWEVTTDRAQLEEAGIVRVLYKPFRIEQLTEIIRSAVQI
                     SPI"
     gene            11736..13079
                     /locus_tag="PKM94_05540"
     CDS             11736..13079
                     /locus_tag="PKM94_05540"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD66138.1"
                     /translation="MDRIGIIEQIRRNDRLLSIPQVLSKVLEEVGKENFSPDSLAKVI
                     LNDPNLTSRILHLVNSPFYHRLAEIKTVNQAISVMGVTTVKCLALSTSIFHPDKVSAE
                     SGIDAKAFFQYELAVAAAAEQTAKAVKYRSPEEAMIAGLLQDIGVLFFLHHYPREYRR
                     VAQRKDKSCTLAEAEREVFSIDHTEVGGYLAEAWKLPEYIRAAIREHHETAMVGEQQT
                     LSNIVRMAVLLTNDHFSGYEPRIEQRLEDINRVSRGLGIEKKTLDEISSSLLAATIST
                     AEFLGVDIGNVEEMLVKANQEIWKSYLTIENLFKERQALTQKLLAEERAKGAIESKNI
                     AMATLSHYLNNAVMAIYGRSQLLRMQHGKGAAASPLLEKLPSNLDVIDRSVKKIVAVL
                     EEMKELSPIDKQKFYNMSEALNIDDRIARRMESMETSGDIGGVLTAAEEAAHLGS"
     gene            13111..14310
                     /gene="aroC"
                     /locus_tag="PKM94_05545"
     CDS             13111..14310
                     /gene="aroC"
                     /locus_tag="PKM94_05545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOD66139.1"
                     /translation="MLTYVTAGESHGPQLTAILDGLPAGFRIDPAEVNYQLARRQKGF
                     GRGGRMKIERDEAEFVSGVRGGVTMGGPITLVIRNRDWENWARIMHPLEPMPGELDLR
                     DRQRTAATRRPRPGHADLPGAVKWNHHDMRNVLERASARETAARTALGALARQLLEHF
                     GVEFASHVVRIGEVEVPRGYDLSDLTRVREVTEASEVRCLDAAAGERMIEAIRAARAV
                     RDSLGGIVEIIVRGLPIGLGGFSQWRQRLDGRLAGALMAVPSVKGVEIGLGFAAAGRR
                     GSEAHDEIFYDPDGDPRRKRFLRRTNNAGGIEGGVTNGEDVVVRVAGKPLSTLNRPLR
                     TVDVATKEPGEAMVERTDTCAVPALGVIGEGVAALVLAEAFLEKFGADNLAETERNFT
                     TYLDTQF"
     gene            14420..15556
                     /locus_tag="PKM94_05550"
     CDS             14420..15556
                     /locus_tag="PKM94_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66140.1"
                     /translation="MRRMLVPAAAAVLTAALIVGCGDGDEPTGPAPDQWYTEAVDTAG
                     RTGLFTSIAVDGSDRPHLVYYNEFGPDNLKYAHYDGAQWQFAVIDANTATSDLAPPVI
                     LVDRAGGSHVAFIDQTADEVRYASELVNGWQVETVDGAGERFCALSMTSTADGVPVIS
                     YGKTISDSVAGLWLAVLDVQADAWEIAAIDSGAAIVASSAAIDSQGLCHVAYIGNAGY
                     PGFDLYYAVGSPGAWAIDTVVGDTTAWYQCSLGVTPDGEPQIAFNSVDPAGMAYAVKT
                     ETGWSIEVIDGAALGPAPEMRTPDGEQLSLRLDAGGVPHLAYWSWADSSLVYAVRGTG
                     WAAERADNSGSVGAYCSLALDHQGRPHIAYQDRDRADLKYAIKR"
     gene            15598..17106
                     /locus_tag="PKM94_05555"
     CDS             15598..17106
                     /locus_tag="PKM94_05555"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOD66141.1"
                     /translation="MILKYSAEDPAGTAADCLVLCVTQYDKPVDPYLKRLDAGTRGGI
                     TRLRQTEEFFGKDGQYAVILSPEGFAAPQVVLVGVGERRVAGHDAYRRALGRIANLPA
                     LTRAESLAIHLGTATRADCFQAAAEGYLLGGWKQREFKSGEEAKDPRRTTRITFMVKG
                     RAQLRPLQQAVERGWTIAEGQLLVRRLAGTPSNRLTPSLYAQEAAALARRHKIEVTVL
                     DEAAIKKEKMGALLAVAQGSAQKPRFVVLRYHGGGRGQKPVVLVGKGVTFDTGGISLK
                     DPLNMHEMKGDMAGSAAVLAALVTAARLRLRINLVGLMPLAENMPSGTATRPGDIVTS
                     RKGKTIEIINTDAEGRLLLADALDYANTFNPQAVIDIATLTGATLYILGYAGAPILGN
                     HEGLLARIKAGSAATGERVWPLPIWDDMREQMKSPIADLTNSGGKPAGTIAAAAFLEN
                     FVGDWPWAHIDIAYVDVEPAGRPYVPKGPTGFGLRLLVDLLMRWKKLEGKKR"
     gene            complement(17204..18295)
                     /gene="traF"
                     /locus_tag="PKM94_05560"
     CDS             complement(17204..18295)
                     /gene="traF"
                     /locus_tag="PKM94_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HOD66142.1"
                     /translation="MECRTIYRLAAILALSGLIASPATASENVGFKDIRSLAMGGTGV
                     AHQANFNAVVFNPALLAKAGFDLHIVSLQVTASQDIKDLVEFIDDNADSLDNFTDISD
                     EAQQQLLDDMAPFDNKWMGAQAAPQIGFATRGFALGVYGTGDVDFRVDKGIIPPRIGA
                     KGRIDRVIVGAAALKAPTAVANILPGQTWIGGTLKIIERRETSLETSFEDADFESVVD
                     SLEEYTTNGWGFDVGLLWDAPGGGLTLGANLVDLIGSLGDDSPPMVVNVGGAYRLSEK
                     LTLAADIDDLFFHEGMSFFNRVYIGGEFLPAKFFALRGGFGQGYPSFGMGLDFSVLRL
                     DAAVYGLERTDNPGGESDYNYVARLVLGY"
     gene            complement(18297..19217)
                     /locus_tag="PKM94_05565"
     CDS             complement(18297..19217)
                     /locus_tag="PKM94_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66143.1"
                     /translation="MYRRLPVLLAVVLSAGLLTVGCNIFDWAAEDDPIAEGNALMREG
                     DYAAAAEQFAKAMDEDPENSDARYYHAKAVIHASGFNALRLGSELEEDEFASNDLLPF
                     FGDNWPDEKANRLFGAVRTAVDDLRPIWDGKTHGNFGPRDIDLDLALLVGMRSILQWR
                     DIDFDGTIGPDDFQFDIEWFEGFNADGFALKNLAEYISADKSAGDADLLCNPAHVAIF
                     NELVDSVSTNIDFTADVLTQILSDSVGVDIDVDVVKEMLGFVKDVAHMYKVNDGDDND
                     YDGQYNEEEIDLIDNDGDGWIDEDSQCQEI"
     gene            complement(19682..20710)
                     /locus_tag="PKM94_05570"
     CDS             complement(19682..20710)
                     /locus_tag="PKM94_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66144.1"
                     /translation="MRSTDNRTARLLATLVAGLALAASAAAAEKLPPARAGKIDPPTG
                     QIAFLRNGNVWIMNADGSGQQLVSQVGNADGSLSWAPDNRRIAFVRSGKIDLRGPDPN
                     VGGLHKVYDIFIAWLDSAYANNTLWWTRLSEDLGSRGPEWSLDGSEIVFWKDMNANYA
                     NAFLPNFQICTMSPDGGKFNILRKDWQNFSNDFLTFPTANRKGQIACVHMSDLKGKGL
                     VVFDRANPMISIDSIRAASAKNLRCIAPSWSPDDQWLAYILDDISAPQVCIATPDLKE
                     HFTVFSPPVGIMLYTNPPSFSPDSKWLTFATSDGSVWICDITGNGARRLTPPGLDKNP
                     AWSKPQPR"
     gene            complement(20798..21646)
                     /locus_tag="PKM94_05575"
     CDS             complement(20798..21646)
                     /locus_tag="PKM94_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66145.1"
                     /translation="MRRFVCLVSAFALLVSAAGTLSAETEQEIVNRYMKKVEKKHVQK
                     IGWVALNYTLNRINRNNDYNSFASSISAQIAEADLPWLGQASTFGVDFAVGVKPRLAW
                     TVGGEYWLKFGSDIAGPLTYSPPSGGATVVDNLTSEIKVYGVTTGLQYFLLNAPAPDR
                     PAAGLSARAFGAVGYYHASWELWPEYQNLNLSTAASESENSTFEGNAPGFTLGLGGEI
                     PLNVLNLSLGADFGYLYLNFTNIAWYNAQDEEIIVTYTGDQDGRVDLDLSGFRGKVEL
                     KRYFAF"
     gene            complement(21885..25451)
                     /gene="nifJ"
                     /locus_tag="PKM94_05580"
     CDS             complement(21885..25451)
                     /gene="nifJ"
                     /locus_tag="PKM94_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOD66146.1"
                     /translation="MVTIDGNTAAAYVAHATNEVIAIYPITPSSNMGEIADEKSARGE
                     KNIWGVIPEVIEMQSEGGASGAVHGALTTGALTTTFTASQGLLLMIPNMFKIAGELTP
                     TVFHVSARAVASHALSIFGDHSDVMACRSTGFGLMASGSVQEVMDLALITQAASLGSR
                     VPFVHFFDGFRTSHEVQKVEEITIDDMAAMLDHDLIVAHRRRALSPDNPTIKGTSQNP
                     DVFFQHRETVNRYYLAAPALVQKAMDRFAGIVGRGYHLFDYMGHPEADRVVVVMGSGA
                     EVVHETVDALAARGEKVGVIKVRLYRPFSVAHFVQALPKTVRRIAVLDRTKEPGSVGE
                     PMYTDVQTAIREALDKGIAPFSQPPLVIGGRYGLGSKEFNPPMAKAVFDNLAAARPKD
                     HFTVGIVDDVTHTSLEPDYSFDLEGEAFRGLFYGLGADGTVGANKNSIKILGENTDNY
                     AQGYFVYDSKKSGAITVSHVRFGKNLIRKPYLIRSAQFVACHNFSFLEKYDMLTPLLP
                     GGTFLLNAPYGPDEVWDRMPREVQQQLIDKKARFFVIDAISLAKELGLGARINMIMQT
                     AFFAISDILPRDEAITAIKHAIVKTYGAKGEKVVKMNFAAVDGATDALREVKYPHKVT
                     STITRPPIVPAHAPEFVKNVTAEIIAGRGDDLPVSAFPDDGTYMTATTQYEKRNIAVD
                     IPIWDREICTQCNICSIVCPHAAIRPKVYEKKDLAGAPAEFPFVEAKTKQLAGKYYTL
                     QVAPEDCTGCEVCVNACPVEAKDAQGTKTGRKAINMTLQAPIRERERANCFAFFLSIP
                     ETDPSLYKRDSVKGSQFIRPLFEFSGACAGCGETPYLKLMTQLFGDRAICANATGCSS
                     IYGGNLPTTPYCRRADGRGPAWSNSLFEDNAEFGLGMRLTADKLQDYALTLVRELVPD
                     MYDALASADQSEQAGIAQQRERVAALLARLDSLGDSDKVATLRSIAYYLVKKSVWVIG
                     GDGWAYDIGYGGLDHVLASGKNVNLLVLDTEVYSNTGGQMSKSTPRGSVAKFAAAGKP
                     RGKKDLGMMMMSYGSVYVAQVALGASNGQTVKAMIEAERYNGPSLIIAYSACIAHGID
                     MAHSMDQEDKAVRCGHWMLYRYNPDLIEQGKNPLQLDSKPPSIPFSEYAYSEIRFRSL
                     KAIDPERAAMLLKLGQQDCDRRWRLYSQLAGLDYSLPPRAGD"
     gene            complement(25562..26125)
                     /gene="dcd"
                     /locus_tag="PKM94_05585"
     CDS             complement(25562..26125)
                     /gene="dcd"
                     /locus_tag="PKM94_05585"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOD66147.1"
                     /translation="MAVMADRWIIEMAERRRMIEPFARDQIRAGISFGVSCYGYDFRL
                     ADEFKLLDTTRVAELDPKNVRPDCWMDARAESLAIPANSFILARSLEYFRIPRNIITL
                     CTGKSTYARSGLVVNVTPFEPEWEGYATLSLANTAPVPLRVYAGEGIAQILFLQGDQD
                     CLRSYRDKSGKYQAQKDITVSRADDSR"
     gene            complement(26229..26654)
                     /locus_tag="PKM94_05590"
     CDS             complement(26229..26654)
                     /locus_tag="PKM94_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD66148.1"
                     /translation="MQFTKAEEYGILGVLYLAERNDATVTPLSEISEARAIPEKFLAK
                     IFQSLSRAGIVRSHRGVRGGFTLAKGPADISVKEILEAIQGPYHLIKCIKDNAICEKT
                     DFCALRELLRAAEDRLVSVFEEHSLADLLSWEKNKAATI"
     gene            26827..27459
                     /gene="cobO"
                     /locus_tag="PKM94_05595"
     CDS             26827..27459
                     /gene="cobO"
                     /locus_tag="PKM94_05595"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOD66149.1"
                     /translation="MTAVDFERQCSTLRLSGLHRHKDRKVMAETEREKKNTGLLIVYT
                     GNGKGKTTAALGMCVRAVGYDWQVCVIQFVKGSWKYGELKGIKRLAPNVELHTVGEGF
                     VGIVDDTKDFEEHRAAARKGVALALEKIRSRAYPLVILDELNVAVDLGLVTDDEVREL
                     LAARDAEQSLVVTGRGARDWLVEQADLVTEMREIKHPFQKGVLAQKGIDW"
     gene            27569..28534
                     /gene="trxB"
                     /locus_tag="PKM94_05600"
     CDS             27569..28534
                     /gene="trxB"
                     /locus_tag="PKM94_05600"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOD66150.1"
                     /translation="MSTAEKNYDIVVVGAGPGGLCAGLYGARARRKVICLEKYMPGGQ
                     IANTEEVEDYLGFVRISGHELATRMAEHAMKFGLEVGSEEVEEIYVDGDDRVVQCASG
                     TRYRAKAVILATGGSPVKLGVPGEREYAGRGVSYCAICDGAFFRDQVIAVVGGGDAAV
                     EEGAFLTKFGSKVHIIHRRKELRAQKIIQQRAKDNPKIEFVLETVVESINGDGRKISD
                     LTLRNVTTGEVSTLPVGACFIFVGFRPNSGLTREELRKDAGGYIITDDKMETSIPGIF
                     ACGDVRAQLVRQITNAVGDGTTAAVAAEKYIEHLEDKRTAQSASR"
     gene            28991..30913
                     /locus_tag="PKM94_05605"
     CDS             28991..30913
                     /locus_tag="PKM94_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD66151.1"
                     /translation="MSRLSHLIIVQVVFVFAALVLIIFSPGHYLSGRAETPRLPDFAP
                     VAAAAAAEAAGRMPADYRTEGAGGQALAALLGGHTAVRQAVLYELAAGRPAALWGYGR
                     EEDSPLPPSWTDHELTHLRLSAADTAGADTHGWPTVPLRLTEQFIVYGAPVEFRDGSS
                     GLLVAAWDHNLFVSDRRALGYALLVLFLCAGLVALLTVYLIHNQFTQPFRRMIHRFER
                     TSDGELYHMLENCGEGELNRLAAVFNKFSRTLLADQRELSRYDAQLRDASSSLEDSQR
                     LLAAVIDHSPVGVITTDAGGRILLFNRRAGAIFGYRAAEALGRPIADLFVNDVQAQAQ
                     AEGEYEDEHGLEVVGRRRDRSQFAAYLVASALRTDAGAGIGYVYIVRDISQRKDFQAM
                     IVRLDRYYTRGEMASEIAHEINNYLSILMGNIELVPLLLRKGNTERLEQKLEVMHGTV
                     EKIARFTDGLLDGSHDQIQFARCDINQLVQAVVAFLQPQNRFDNTAIVCELSPDIPLV
                     EADPGLLQQLLVNLVYNAGDAVLGSEGPRRIEIRTFPAAGAGAPTVGITVRDTGPGVA
                     PDKLPLLFKRRFTTKPRGHGIGLIACRKILDSHGGDIAYAHDGGAVFSVTLPIEHRSR
                     ESAASPAAAAADIPQV"
     gene            30956..>31163
                     /locus_tag="PKM94_05610"
     CDS             30956..>31163
                     /locus_tag="PKM94_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66152.1"
                     /translation="MAERANGKPTWVERRIPAIGAIVLAVAVGVYAWIGISESRKDSL
                     GLLVTQGAAFTEALAFVFQAQDMEL"
BASE COUNT         5411 a         9069 c        11211 g         5472 t
ORIGIN      
        1 acgtggtcga tgaaatcacc catctcgacc cgagctactc gccggagcag gtgacggtgc
       61 tcaagatgaa ccagaaggat ccgtcactct tggcggaccg ggcggcctcg atattccact
      121 tcccgctgcc ggccaagcag gaaatcctcg aatcggtcga tctctcaatg cgctatgacc
      181 gcctgctggc ctcgctcaac agcgaactga tgcgggtggc gacggcggcc tcggtgaacg
      241 aaaatgtccg aaggaacatg gccgaggaag agcggcgggt gttcctgcgg cagcagttgg
      301 cggagatccg ccggcagctg ggggaggatc cctcggagga gcgggaggcg gcggccctgc
      361 ggcaccagat caagtcgtcg aacagccttc cccccgacgt ggtagcgcgc gcgacgatcg
      421 agatcgaccg cctgtcggag ctttcccccg cctcggccga gtacggggtg accaagaact
      481 acctgaactg gctgttgtcg ctgccgtggg ggaaggtgac gccggaggac tactcgatgg
      541 cggaggtggc gcgggtcttg tcggcggagc actacggtcc gacacagctc aaggagcaga
      601 tcctgcagcg gctgtcggtg cgcaagctgc tcggggggac gagcgagggg ccgacgctgt
      661 gtctgatcgg cgcgccgggg acggggaaag cgtcgctggc caaggcgatg gcgaaagcca
      721 tgggcaagcg cctggtgcgg atctcggtcg gggggatctc ggaggtgtcg gagattaagg
      781 gttcgccgcg gactttcctc ggggcgatgc ccgggaaaat cgtccgggcg atgcgcgacg
      841 cggggagctg tgacccggtt gtcctgatcg aggatatcga ctacttcaac atggacaacg
      901 actcctcggt caacatggtg ctcctcgagg cgatcgacag ccggtggaac aaggccttcc
      961 tggacaacta cctcggggtg cccttcgacc tgagcaaggc cttcttcatc tgctcggtgc
     1021 ggtcgttcga ggagatcccc gagcagttcg tgccgcggct tgacatcatc gaactgcccg
     1081 cctatatcga aaaagaaaaa atcgtcatct cgaagaagta catcatcccg aagatgttgc
     1141 gcaaacacgg gttgtcgaag gcggaggtgg agtttgagac gaaggccctg acgcgcatca
     1201 tctccaacta cacgatggag gcggggctgc tcgtgttctc gcagcagatc gagaagattc
     1261 tgcgcaagat cgcgctggag aaggcggaga agccgcgcaa gaactgggtg atccggccgg
     1321 acactatcga attgtatctc gggcagccgg tgttcatacc ggagaaggcg gaaaccaagc
     1381 cggagatcgg gtgcgcgacc gggttggcgt ggaccggggc ggggggggac ctgatgttca
     1441 tcgaagggtt gaagatgaag ggggacgggc agatcatcac gaccgggtcg ctcggggacg
     1501 tgatgcggga gtcgatacag gcggcccatt cgtatgtccg gtcaaaagcg gatgtactgg
     1561 ggattgattt caacgacttc aacgaattcg acattcacat tcactttccc tcgggggcaa
     1621 tccccaaaga cggaccctcg gccggaatca cggtgtgtct ggtgatcgca tcgctcatgg
     1681 ccgagcggcc gatccgcaac gagatagcga tgacgggtga agtgacgctt cggggacggg
     1741 tgctccctgt gggcgggatc aaggagaaag tctcggcggc ctaccgggcc gggattctca
     1801 cggtcgtgct gccgaaggag aacgagaagg atttgaagga cttgcccaaa gaaatccttc
     1861 gccgcaccgc attccgattc atagagcggg tggacgagtt gttcgagttg tgcctgctgg
     1921 atttcacacc gtcggcctat accctcgaga agatcttcgc cgaggagatt gagaaggcga
     1981 agcggcggtc gtcggcgggg aaggcggcgg cccgcaaggt gtcgaagaag ccgggcggga
     2041 agccctcgac caagcgtccg cgcacgcggt agtctcacat tccaattcat gggtgcggga
     2101 ggggctcagg cagggccggg gttggcgatt gcgccccgcg gggtctctat ttcccttgac
     2161 aggcgggggc cgtcggagta tatttaagca gcgtgtaatg ggcagaaagt cctccgtgtg
     2221 tccggcagtc aacctcagag ttatgaaccg gcaagacgtc aggtgtcaat cgaagtgagt
     2281 gcgggaagag cggtccgaag gtctggtcgg cccgatacac gcggcggtta tgggaatcag
     2341 taggtgcggc ccttcggccc gtggcggaag tgacggcccg ccgccggggg ggtgccgggg
     2401 ttaaagagac gataaacgga attcgcatgg aggcacaccg atgaaaagca agctgctcgt
     2461 gctgatgctg atcctgttgc tggcgggcgc cggcccggcg gcggcgcagt ttcagcagga
     2521 tccggatgat gccggcgccg ccgacacaat cgacctggtt tattccgtgg tgccggacca
     2581 cgccaccaat caactgcatt tgcgggctga tctgtatggg ttcacggacg ccaacaccat
     2641 attcggtgtg acgatggggt tcaagtggat caacccgaac ctgcagatgg acagcgccaa
     2701 ttcgccggcg gacatccgca ccgctttcga tttggggacc ttctactacg agaacgcgaa
     2761 catcgcgctg accaacacca accatcggtt cattttcggc ggtttgtcgg ctttttcagt
     2821 cggcttgcag ccggcgccga accgtcgcct ctgggcgagc tacttcttca ccctgagcag
     2881 ctggggagaa tgcgacaccg tctccatcga cacgctgttc tggcagccag ggacggaatt
     2941 caacctggtt gacgataaca acaacgagat cattccctac tggacgggcc gggagcggta
     3001 caaggacact tcctgcgccg agccgtcgaa cctgatcgtg acgccggatt cgctgttttt
     3061 ctcggccgtg cagggaggcg gggcgccgtc ggggcagcag ttcgtcatca gttcggacaa
     3121 cgagccgctg gccttcacgg tgaccgagac gtcgacctgg atggcggtga gtccgctggc
     3181 ggggaacacg ccgcggagca tcacggtgtt gccgaacatc gtgggcctgg cccccgccac
     3241 gtacatcgat tcagtcatgg tggcggcgcc ggcggcggcc aacacgccgc agtgggtgaa
     3301 agtggtgctc ttggtgcagg agccgccgcc gacgatcgcg gccacgccga cggcgttctt
     3361 tttcaacgcc gtcgcgggcg gggcgaaccc ggatccgaag acactgacga tcaagaacac
     3421 cggcgggcag gtgctcaact ggacgctgag ccacagccag ggctggttgt cgctggcgcc
     3481 gatggcgggg acggactcgg cggcggtgac ggtgtcggtg gatatcacag ggctggcctt
     3541 caacacctac tacgacacgg tggtgattac ggcggcgggg gcgaccaaca ccccggtgcg
     3601 ggttccggtg acgctctcgg tggggtcgga tctgccgatt atcgcggtcg attcggcgtt
     3661 caactacgtc gtggtgccgt ggccggcgcg ggaggtcgga ccgtggacga taacgatccg
     3721 gaatgacggc gcgggggcaa tgaatttctg gctggaggaa aacgccagcc gaattctgag
     3781 catgtcgccg atgtccgggg cggcgccgct ggacgtccag gtcacgttca agctgaacgc
     3841 gggcaatgcc ggggtcgact actacgacac gctttgggtg tactcgaatg aggccatcaa
     3901 ttcccccttc ccggtggttt tcctgttcca ttatgtgtcc gatcccgccc tcatgatcgt
     3961 gaccgccgac accgtgcgcc tgacgctgta tgagtgcgag atggggatac gcaacctgcc
     4021 gccggtcggc gggttcatcg tcgacaatgc cggcggggac gatccgatgg agtttgtgct
     4081 cggttgggag tctgagttgt tcgatatctc gaccctgagc aacgtggcgc ccgcgaccgt
     4141 ctcggtccag gcgaatttcc tgaatctgcc gatcggggtg tactacgaca ccattgtcgt
     4201 gacggcgcgg aaatcgacca atccggcgga cacggtgatc gtgaagtact cggtcatacc
     4261 gccgacgcag cagcccaaga tctgggtttc gaacagctcg tttgtcgtgc cggcgcagga
     4321 gaacagcggg ccgctgccgg atatcgggat ggcgattcac aatgaattcg gcggctgcat
     4381 gccgtggtac atcgtggaaa acgtcccctg gctgttcccc tccgacacct ccgggatcaa
     4441 cccggcgtcg ctgatcttcg gggtgaattc ggacgggttc ctgttcggag agtatccgga
     4501 ttcgatcaag ctgtacgctc cctcggcctc caacagcccg ggcacaatcc gggtgcgcct
     4561 gaaagtgtgg cgttttcatg gggactgcga ctacaactcg gtgatcaacg ttcttgacct
     4621 cacctacatg atttcgttcc tgttccgggg cggtccccag ccgcagccga cgcagttggt
     4681 cggggatctg aactgcgacc acctggtgaa tatcgccgac ctcacgtatt tcatcgaata
     4741 tctgttccgc aacggtccta taccgtgttg gaacccgtac aagtgacggc ccggtgaagg
     4801 ccggctggag aggcgccgcg gcggaaagcc gcggcgtttt tctatgggcg cggggcaaaa
     4861 tgtgcgcctc ggctaacagc cgaattggcc ttgacttacg gggcggatcg gttattttgc
     4921 ggaacgatcg acgctccccg tacatacagt tgtaccttgg tccgccgacg atgaataaca
     4981 agtgatcggt ccatagagag tttggaggca ccattgagct cagcatcggt gacacgcatc
     5041 ctggcggttc tggcggcgct tgtgctgctt gcgacggccg ccggtttcgg ggatgacgat
     5101 gagagccggg actggaacac cgccccggcg tcggaaattc acgagcgtct ggcggcggcc
     5161 aaggcggggg acctgcagcg gcgcaccgcc ctgaaacagc tcctcggcgg gtccgaagcg
     5221 accacgcaga cgaactacga cgtcacattc tacgatgtga agctccggat caacgacacc
     5281 accgagatcg tgtgggggcg ggtgacgttc gtggcgacgg cggccgaggg gaatgtcggc
     5341 agcgtggagg tcgatctgtt cctcaacatg acgatcgact cgatcgtcgg ttcctcgggc
     5401 caactggctt tcacccgcct gggcaacgtg gtgacgatcg acctcgacgg cacctaccgg
     5461 gtgggcgagc agttcgcgtt cgatttctgg taccacggcc acccgacgga atcggccggg
     5521 ttcggcgcgt ttacgttcgg cacctacggt gcgtacacgg cgatttcgac cttgtcggag
     5581 ccctatttcg cgcggacctg gtggccgtgc aaggaccgca tggacgacaa ggcggactcg
     5641 ttcaacatcg ccatcgaggt cgacacttcg ctctacgcgg cctcgaacgg cacgcttgat
     5701 tcgatggtag cccacggcgg caacacgcac acgtactact accgggtgcg ctatccgatg
     5761 gcgacctacc tgttcatgct ggcgatttcg gagttccagg tgtggtacga ccaatgggtg
     5821 tacaacgggg gcgccgacac gatgccgctg gagtacgccg tgtacccgag ctggtacacg
     5881 gcctcgctgt cgagtttcgc ggtgacgccg ggggcgctga cgattttctc ggacgttttc
     5941 gggccgtatc cgtttgcgac ggagaagtac gggcacgcgc actttacctg gggcggggcg
     6001 atggagcatc agacgatgac ctcgctgacg gccaacagtt tctccatgtc aacgacggtg
     6061 atagtccacg agatggccca ccagtggtgg ggggacttga tcacctgcaa atcctggcgc
     6121 gacatctggc tgaacgaagg gtgggcatcc tacgcggagg cgctctatta cgaggcgctg
     6181 ggcggctgga cggcctacat caatcacatg ataggcatgt acttcacggc cggcgggacc
     6241 gtctactgcc aggacacttc gagtacggat gcgattttca cgagccgcgt gtacgacaag
     6301 ggggcgtggg tgctgcactc gctgcgcggg gtgctcgggg attcgctctt ctttgcgggc
     6361 gtgcacgcct actacaactc cccctaccag ttcaaggccg cgaccaccga ggatttcaag
     6421 aacgtgttcg aagcggcgac cggggtggaa ctggactggt ttttcaacga gtgggtctac
     6481 ggcacgtact acccgaattt ctggtggtac tggatgagcg agccggccgc cgggggcggc
     6541 tgggacgtct acctccgcgt ccggcaggtg cagaccaccg agccgcgggt gttcacgatg
     6601 ccgatcaagt tcacgttcac cggggtcagc gacacggtga cggtgtgggc ggacgagaag
     6661 atcgagcgga cgaagattca tctgccggcg aacccctcgg cgctgagtct cgacccgatg
     6721 aactggatga tcaagtcgca gcagcggctg gcctgggaca tgttcgtcat cacgccgagc
     6781 gacggtctcg atacggtgcg gcaatacagc gcctacaccg ataccatcga gctgcggggc
     6841 ggctccggct ggcagacgtg gtcggtgacg gccggcagtc tgccgaccgg tctgagcctc
     6901 tccaacgagg gcgtgatcag cgggaccacg agcgacaccg ggcggtttgc ttttacggtt
     6961 ttcgtggacg acaacagtta cagctacacc gaccaggtcg atttcacgct ctacgcggcg
     7021 ccgagcgccg ggaagcccgg ggacgtgacc aacgacgggc aggtcaacgt ggccgacctg
     7081 acgctgctga tcgactttct cttccggggc ggtgagccgc cggtgattct caactcggcg
     7141 gatgtcaacg gttcctgcga catcagcgtg gccgacgtga cctacctggt ggcgcacctg
     7201 ttccgggggg gaccggcgcc tctgcccggg tgtgtgaact gacccagggg ggaccatgag
     7261 ggttggggcg gcggcctccc ggcggggccg ccgtcgtcgt tttcggccgg gtttttgatt
     7321 gcgccgaggc cggaaaaagg tatcattagg gcagtccgcg cgcgaccgtt ctcgccggca
     7381 aaggaattcg aaaagcgatg gccaccacca gggacatacg gacgttccac attacgactt
     7441 tcggctgcca gatgaatctg gcggactcct cgaccctggc cgcctcgctg ctcacgcggg
     7501 gataccgccg ggcggccagt ccggccgagg ctgacttgct cattctcaac acctgctcgg
     7561 tgcgggagaa agccgaacag cgggtgttcg gccgactggg cgagatttcc caactcaagc
     7621 ggcggaagcc ccatgtgaag gtggcggttg tgggctgcat ggcgcagcgg ctgggcgacg
     7681 aactggtgcg ccgggccccg caggtcgaca tcgtgctcgg taccgaccgg ctgttcgagc
     7741 tgcccgacgt cctcgagggg atcgagggga cgccgcggat tatgacggcc ttcgggcacg
     7801 aggatatgga ctcgatcgtc ccggtcaaag agacgccctt ttcgggcttt gtaactatct
     7861 cgcggggctg cgacaattac tgcacgtact gcatcgtgcc gtacgtccgg gggcgggagc
     7921 gggcccacgc ggccggattc attctcgacg ccgtccgcaa aatggcggac gagggggtgg
     7981 tggaggtgac cctgctcggg cagaacgtga acagctacgc ctgggaggga atcgggtttc
     8041 ccgagctgct gttgcgggtg gcgcgggaga ccggggtgca gcgggtgcgg tacatgacgt
     8101 cgcatccgaa ggatctctcg cggcggctgg ttgacgttat ggcggacgag ccgaagatca
     8161 tgccgcacat ccacctcccg ctgcagtcgg gcagcgaccg catcctgcgg cggatggggc
     8221 gtgcgtacac gctcgaacac tacctgaaga tcatcgacta catccgcgcc cggctggcct
     8281 acgcggcgct caccaccgat ctcattgtcg gttttcccag cgagaccgag gcggaattcg
     8341 aacagacgct cgcggcggtg cggcggatcg agtacgacgc ggctttcatg ttccgctatt
     8401 cggtgcggcc ggggacgacg gcggcgcgct cggccgacga cgtgccggag gcggagaaga
     8461 tccgccgcct gaacacgctc atcgcgctcc agcagcagat ctcccacgcc cggaaccagc
     8521 gcgaggtcgg gcaggtgcgc tcggccctgg tcgagggacc gtcgcgtcgc gacccgcggg
     8581 tgctgcgcgc ccgcacggag gggaacaaga cggtgctgtt cgacagcggg cgggcgaagc
     8641 cgggcgatgt ggtggcggtg cgcatcgcgg ccgccgacgc tttcacgctc cacggcgaga
     8701 tggtcgaggc cggcgcatga tcgagcatct cccgatcatc ctcctgccgg cggcggtggc
     8761 gctcaccgtg atttttctca cggtgcggct gcggttgttc gccgacgagg aggccgcggg
     8821 gaggtggctc tttgtggcgg gcggggcggt gctcacggcg gcggcggtct ggcacggcgt
     8881 gcggcagacg gcggcctacg ccgactggtt cgtggccgcg gcctacccct atctcgacct
     8941 cgcgcacttt gccgtgatcc tcgccgggct gctcctggcg gcggcgggcc tggcgttcta
     9001 tgccgacggg tggcagacgc ggcgcgagga catcgaggtg cgcgaccaga agctgtcgat
     9061 cctcgacaac ctccagcggg acgcgcgcgg accgtaccaa ctcgtggacc tgctctccat
     9121 gtcaatccgg gagatcgtgt cgcaccttcc ggacgcggcg ggggcggtgt tcctgctcaa
     9181 ccggaagcac cggcagttcg tactcaccgc ctcggtcggg ctctcccagg aggagaccgc
     9241 gctgctggag tatttcccgc tccagcggaa taccgtcagt cagagtatcg atatcggcga
     9301 gccgattctc ggtcggagtt tcagcttcat cgagcgttcg ggggcggagc ggccctcgcg
     9361 ctttcagtcg gtgctggtgc tgccgctggt gtcggggatg gagaagatcg ggggcatcct
     9421 gctggtggcc gaggcggagc actatttcag ccgggcggag atccggtatt tacagccggt
     9481 ggcggagtgg attgcggaga agatcaagtc ggcgcggctg gagcgggagc tgacagcggc
     9541 ggtgcggcag agcgaggagc gggcgcagcg gggggatgac ctggcgaccc gtctgcgggc
     9601 ggcaacggcg gccctgggcg cgcgcgacgg ggtggcggct ttctgccggg cgctcgcggg
     9661 cgtcgccgag agccgctccg tccacctctt cgggctgacc ggcggagcgc tgcagtttta
     9721 cggcgggtcg gaatcgctcg gggacatttc ggagaattac cggacggcgc tggtggatgc
     9781 gctcgaccgc aacaagccgc tcgtgatcaa ccaggagggg gtgggcgacg gcgggcggaa
     9841 attcgtggcc gtgtccagtc tcgtggcgcc gctgggcggg cgggaggcga gcgcgctcct
     9901 tctgcggcgg gaggacgggc cgttccgggt cgacgatgac gatctcaaac agcttgagct
     9961 gttcggaggt ctcgcccggc tggcgctcca gcgggcggac accgagcggc tcgacctgac
    10021 gcggcggaag gggttcgaga cggtgctgca gttgctgcgg ttcgatgtcg acgtcgagcc
    10081 cgacaagcag cccgatttct tcacggcgca catggccccg gcgctgccgg cggggagtct
    10141 ggccgtcctg ttccgtcggg agaacgacgg ctcgttcacc gctgtcggcg gcgcgccggg
    10201 ggcggcggat ctccgcttgc tgccgggcga ggggatcgtg ggggaggcgg ccaaccgcgc
    10261 cgaggggcgg ttcgtgttca gccgggcggc ggtcggacgg gcgatcgagg ctttggaatc
    10321 gggatcgcgc gagcagatgc aggcgctctt cggcgagcag ggcgtgccgg gcttcgccgc
    10381 ggcctgcccc attttgaggc tcgatcgcgt ggccgcggtg gccctctttc tcctgtacga
    10441 cgcgaccgag agcgagcggg gagagtggga gcggctgctc accctggcct cctcgctgta
    10501 ttcggtgcgg ctgacggtgg ccgagctcaa ccggcgggtg ctggaggcgc agacgacggg
    10561 gggcggcgtg gatcaggatc tcgtggggcg gatggtcaac gagttgaaca accacctctc
    10621 cgccgtgatc gggaacgccg aactcgcttt cacgcggacc aatctcacgg gggatgtggc
    10681 gcagcatctc cgcagcgtca tcgccgaggc cgaacaggcg gcgaaagcgc tgaaggattc
    10741 gctgggacgg atgcggctgc cggcgccgtc gacggcggtg gcggcggtcg aggacaagtc
    10801 gctcggggcg gtggtgaaag cggtgctccg ccgcctgcag gtggccgaaa atctctacct
    10861 ggccggcggg cgggcgcgcg aaatggccgt gctcgccgaa gccgacggcg tcgtgccggt
    10921 ggccgaggaa cgcctctacg gactggtcga agaaatgctc aaccggttcg gggcggcggc
    10981 ggccgacgac gatgtgctca cgctgcgcct gtatcggtcg gacgaccggc tgtgcctcga
    11041 tctctcgcgg caccaccgca atttcccgcc ggtcgacaag gtggccggct tcggcgagta
    11101 cgagggcgcc ccagaggcgc tccgccagcg gccatcggat acgttcctgc gctacctggc
    11161 cgatagcgac gtcacctacg cccacgaccg ccacacccag ccctcgactt acctgtcgtt
    11221 ccagtttccg acggcggcgc cgggagggtc ggcgcgggcg gcccgtccga aggaagcggt
    11281 ccggattctg gcgatcgacg accagacagt cattctcgat ctgatcgcgg ccatgagcca
    11341 gtcaatgggc tacgaggtgg cggtggcgtc ctcggggccg gagggaatcg cgctggcgcg
    11401 gcggcagcgg ttcacgcttg tgctgaccga cctggcgatg ccggggatgt ccggcctgga
    11461 ggtggcggcg gagattcgca agcttcagcc ggacacgccg atcgtgttgg tcacggggtg
    11521 ggaggtgacg accgaccggg cgcagcttga ggaggcaggg attgtccggg tcctctataa
    11581 accctttcgg attgaacagc ttacagagat cattcggtcg gccgtgcaga tctcaccgat
    11641 ctgagttcga tatttccggt ttcccggggc tcgcggttaa ctcctgaatt cccaagccga
    11701 taacggtagt atgacaggga aaggattatc cgcgtatgga tcgaatcggg attatcgagc
    11761 aaatacgccg gaacgaccgg ttgttgtcca ttccgcaggt gctctcgaaa gtgctggaag
    11821 aggtggggaa ggagaacttc tctccagact cgctggcgaa agtcattctc aatgatccga
    11881 acctgaccag tcggattctc catctggtca attcgccttt ctatcatcgc ctggcggaga
    11941 tcaagacggt caaccaggcg atttcggtca tgggggtcac gacggtcaag tgcctggccc
    12001 tctccacctc gatcttccac ccggacaaag tctccgccga atcggggatc gacgccaagg
    12061 cgttcttcca gtacgagctg gcggtcgccg cggccgccga gcagacggcg aaggcggtga
    12121 agtaccggtc gcccgaagaa gcgatgattg ccggtctgct ccaggacatc ggggtgctgt
    12181 tcttcctcca ccactatccc cgggagtacc gcagggtggc gcagcggaag gacaagagct
    12241 gcacgttggc ggaggccgaa cgggaggtgt tcagcatcga ccacaccgag gtcggggggt
    12301 accttgcgga ggcctggaag ctgcccgagt atatccgggc ggccattcgc gagcaccacg
    12361 agacggcgat ggtcggggag cagcagacgc tctcgaacat tgtccggatg gcggtgctgc
    12421 tcaccaacga ccatttctcc gggtacgagc cgcgaatcga acagcggctc gaggacatca
    12481 accgggtatc ccgggggctg gggatcgaga agaagacgct cgacgagata tcgtcgtcgc
    12541 tgctggcggc cacgatctcg acggcggaat ttctcggcgt cgatatcggg aacgtggagg
    12601 agatgctggt caaggcgaac caggaaatct ggaagtcgta cctgacgatc gagaacctct
    12661 tcaaggagcg gcaggcgctg acccagaagc tcctggcgga ggagcgggcg aagggggcga
    12721 tcgagtcgaa gaatatcgcc atggcgactc tgtcgcacta cctcaacaac gcggtgatgg
    12781 cgatctacgg gcggtcgcaa ctgctccgga tgcagcacgg caagggagcg gcggcgtcgc
    12841 cgctgctgga gaagctcccg tccaatctcg acgtgatcga ccggtcggtg aagaagatcg
    12901 tggccgtgct cgaagagatg aaagagctct cgcccatcga caagcagaaa ttctacaaca
    12961 tgtcggaggc gctgaatatc gacgaccgga tcgcgcggcg gatggagtcg atggagacct
    13021 cgggggatat cggcggcgtg ctcacggcgg ccgaagaggc cgcccatctc ggcagttgac
    13081 cggcaggcgg cggcgccgta ccatggggtc atgctgacgt atgtgacagc cggcgaatcg
    13141 cacggcccgc aactgacggc gatcctcgac ggtcttccgg cggggttccg gatcgacccg
    13201 gccgaggtca attaccagtt ggcgcggcgc cagaagggat tcgggcgggg cgggcgcatg
    13261 aaaatcgagc gcgacgaggc cgagttcgtc tcgggtgtgc gcggcggggt gaccatgggg
    13321 ggaccgatca cgctggtgat ccggaaccgc gactgggaaa actgggcgcg gatcatgcac
    13381 ccgctcgaac cgatgccggg ggagctggat ctgcgcgacc ggcagcggac cgccgccacc
    13441 cggcgtcccc ggcccgggca cgccgacctt ccgggggccg tcaaatggaa ccaccacgac
    13501 atgcgcaacg tgctggagcg ggcttcggcg cgggagacgg cggcgcggac ggcgctcggg
    13561 gcgctggcgc gccagctgct cgagcatttc ggcgtcgagt tcgcttccca cgtcgtacgg
    13621 atcggcgagg tcgaagtccc ccgcggctat gacctcagcg atctgacccg ggtccgcgag
    13681 gtcacggagg cctcggaagt gcgctgtctc gacgccgcgg ccggagagcg gatgatcgag
    13741 gcgatccggg cggcccgcgc ggtgcgtgat tcgctggggg gaattgtcga gatcatcgtc
    13801 cgcgggctgc cgatcggcct gggcgggttc tcgcagtggc ggcagcggct cgacggccgc
    13861 ctggcggggg cgctcatggc ggtgccctcg gtgaaggggg tcgagatcgg cctgggtttc
    13921 gccgccgcag ggcggcgcgg gtccgaagcg cacgacgaaa tcttctacga tcccgacggc
    13981 gacccgcggc gcaagcggtt cctccgccgg acgaacaacg cggggggaat cgaggggggc
    14041 gtcaccaacg gcgaggatgt ggtcgtgcgg gtggcgggaa aaccgctctc cacgctcaat
    14101 cggccgctcc ggacggtgga tgtggccacg aaggagccgg gggaggcgat ggtggagcgg
    14161 accgacacct gcgccgtgcc ggcgctgggc gtgatcggcg agggcgtggc ggcgctcgtg
    14221 ctggcggagg cgttcctgga gaagttcggg gcggacaacc tggccgaaac ggaacgcaac
    14281 ttcaccacct atctcgacac gcaattctag ccccttatgt ggccctccgg ccggtgtgca
    14341 tcgggctgtc gcggctgttg ccccggccgc gggaatccgc tatactgccc ctgaatctgg
    14401 ctgacggaaa ggggtagtca tgagacgaat gcttgtgccg gcggccgcgg cggtgttgac
    14461 ggcggctctc attgtcggct gcggcgacgg agacgagccg acgggtcccg cgccggatca
    14521 gtggtacacc gaggcggtcg acacagccgg gcggaccggg ctgttcacct cgattgccgt
    14581 ggacggatcg gatcggcccc acctggtcta ctacaacgag tttgggccgg acaatttgaa
    14641 gtatgcgcac tatgatgggg cgcaatggca attcgcagtc atcgatgcca acaccgcgac
    14701 ctcggacctc gcgccgccgg tgattctggt cgaccgggcc ggcgggtcgc acgtcgcttt
    14761 catcgaccag actgccgacg aggtgcgcta cgcctccgag ctggttaacg gctggcaggt
    14821 ggagacggtc gacggggcgg gggaacgttt ctgcgcgctg tcgatgacgt ctacggccga
    14881 cggcgtcccg gttatctcct acggcaagac aatcagcgat tcggtcgcgg gcctgtggct
    14941 ggcggttctc gacgtgcagg cggacgcctg ggaaatcgcg gcgatcgatt cgggggccgc
    15001 gattgtcgcc tcgtcggcgg ccatcgattc gcaggggctt tgccacgtcg cctatatcgg
    15061 aaacgccgga tacccgggat tcgatctgta ctacgcggtc ggctcgccgg gagcctgggc
    15121 gatcgacacg gtggtgggcg acaccacggc ctggtaccag tgctcgctgg gcgtgacgcc
    15181 ggacggggag ccgcagattg cgttcaactc ggtcgacccg gcggggatgg cgtatgcggt
    15241 gaagaccgag accgggtggt cgatcgaagt gatcgacggg gcggcgctgg gtccggcccc
    15301 cgagatgcgc acgcccgacg gcgagcaatt gagtctgcgg ctcgacgccg gcggggtgcc
    15361 gcacctcgcc tactggagct gggccgattc ctcgctggtg tacgccgtgc gcgggaccgg
    15421 ctgggcggcg gagcgggcgg acaattcggg atcggtgggc gcctactgca gtctggcgct
    15481 cgaccaccag ggacgccccc atattgccta ccaggaccgc gaccgcgcgg acctgaaata
    15541 cgcgatcaag cgctaacggc gcactcaacg gacggcgtgc gataaatgga gtctcacatg
    15601 atactgaagt attcggcgga agacccggcc gggaccgcag ccgactgcct ggtcctgtgc
    15661 gtgacgcagt atgacaagcc ggtcgatccg tatttgaagc ggctcgacgc ggggacgcgg
    15721 ggggggatca cgcggctgcg ccagacggag gagtttttcg gcaaggacgg gcagtatgcg
    15781 gtgattctga gcccggaggg tttcgccgcc ccgcaggtgg tgctggtcgg ggtcggcgag
    15841 cgccgtgtcg ccggccatga cgcctaccgc agggcgctgg ggcggatcgc gaacctgccg
    15901 gcgctcaccc gcgccgagtc gctggcgatc catctcggca cggcgacgag ggcggattgc
    15961 tttcaggcgg cggcggaagg gtatctgctc ggcgggtgga agcagcgcga gttcaagtcc
    16021 ggcgaggagg ccaaagaccc gcggcggacg acccggatca cgttcatggt caaggggcgg
    16081 gcgcagctca ggccgctgca gcaggcggtg gagcgcgggt ggacgatcgc ggagggacaa
    16141 ctcctggtgc ggcgcctggc cggcacgccg tcgaaccgcc tcacgccctc gctctacgcc
    16201 caggaggccg cggcgctcgc ccgccggcac aagatcgagg tcacggtgct cgacgaggct
    16261 gcgatcaaaa aggagaagat gggagcgctg ctcgcggtcg cgcagggatc ggcgcagaaa
    16321 ccgaggtttg tcgtgctgcg ctaccacggg ggcgggcgcg ggcagaagcc ggtggtgctg
    16381 gtcggcaagg gcgtgacatt cgacacgggc gggatcagcc tgaaggatcc gctcaacatg
    16441 cacgagatga agggggacat ggccggctcg gcggcggtcc tggcggcgct cgtgacggcg
    16501 gcgcgcctgc ggctccggat caacctggtc gggctcatgc cgctggcgga gaatatgccc
    16561 tcgggtacgg ccacgcggcc gggagacatc gtaacctcgc gcaagggaaa gacgatcgag
    16621 atcatcaaca ccgatgccga ggggcggctt ctcctggccg acgccctcga ctacgcgaac
    16681 acgttcaatc cgcaggcggt gatcgacatc gccacgctca ccggggcgac gctgtacatc
    16741 ctcggctacg ccggtgcgcc catcctgggg aaccatgaag gtctgctggc gcggatcaaa
    16801 gctgggtccg cggccaccgg ggaacgggtg tggccgctgc cgatctggga cgacatgcgg
    16861 gagcagatga agtcgccgat cgccgatctg acgaactcgg gcggcaagcc ggccgggacc
    16921 atcgcggcgg cggccttcct ggagaacttc gtcggcgact ggccctgggc gcacatcgac
    16981 atcgcttatg ttgatgtgga gccggccggc aggccgtatg tcccgaaagg gccgaccgga
    17041 ttcgggctgc ggctgctggt ggacctgctg atgcgctgga agaagctgga ggggaagaag
    17101 cggtagagcc ggcgcggccc gcaccggacc gggatcagac aggataatgc ccgttcgcgc
    17161 agcgcgcgaa cgggcattgt ggtatcactc ggcgggtgga agcctagtag cccaggacga
    17221 gccgggcgac gtagttgtag tcggattcgc cgccggggtt gtcggtccgc tcgaggccgt
    17281 agaccgcggc gtcgagccgg agcaccgaga agtcgagacc cattccgaag gagggatacc
    17341 cctggccgaa gccgccgcgg agggcgaaga atttcgcggg caggaattcg ccgccgatgt
    17401 acacgcggtt gaagaaggac atcccttcgt ggaaaaagag gtcgtcgatg tcggcggcca
    17461 gcgtcagttt ttcggacaga cggtaggccc cgccgacgtt gacgaccata gggggagaat
    17521 catcgccgag gctgccgatc aggtcgacga ggttggcgcc gagggtgagc ccgccgcccg
    17581 gagcgtccca caacaagccg acgtcgaaac cccagccgtt ggtggtgtac tcctcgaggc
    17641 tgtcgacgac gctctcgaaa tcggcgtcct cgaacgaggt ctccagggag gtctcgcggc
    17701 gttcgatgat cttgagggtg ccgccgatcc aggtctggcc ggggaggatg ttggcgaccg
    17761 cggtgggcgc tttgagggcc gccgcgccga cgatcacgcg atcgatccgc cccttggcgc
    17821 cgatgcgcgg cgggatgatc cccttgtcca cgcggaagtc cacgtcgccc gtgccgtaga
    17881 ccccgagggc aaagccccgg gtggcgaaac cgatctgcgg cgccgcctgc gcacccatcc
    17941 acttgttgtc gaacggggcc atgtcgtcca gcagttgctg ctgggcctcg tcgctgatgt
    18001 ccgtgaagtt gtcgagggaa tcggcgttgt cgtcgatgaa ctccacgaga tccttgatgt
    18061 cctgcgacgc ggtcacctgg aggctcacga tgtgcaggtc gaagccggcc ttggccagga
    18121 gggccgggtt gaacacgacc gcgttgaagt tggcctgatg cgcgacaccc gtgccgccca
    18181 tggcgagcga gcggatgtct ttgaagccga cgttttccga agcggtggcc ggcgaggcga
    18241 tcagcccgct cagcgcgagg atcgcggcca gccggtaaat tgtcctgcat tccatgctat
    18301 atctcctggc actgcgaatc ttcatcgatc cagccgtcgc cgtcgttgtc gataaggtcg
    18361 atttcctctt cgttgtactg cccgtcgtag tcgttgtcgt ctccgtcatt gaccttgtac
    18421 atgtgggcga cgtctttcac gaatcccagc atttctttga cgacgtcgac gtcgatgtcg
    18481 acgcccacgg agtcggagag gatctgggtg aggacgtcgg cggtgaagtc gatgttggtg
    18541 ctgacgctgt cgaccagctc gttgaagatg gcgacgtggg cgggattgca caggaggtcg
    18601 gcgtcgccgg ccgacttgtc ggcggagata tactcggcca ggtttttcag ggcgaaacca
    18661 tcggcgttga atccctcgaa ccattcgata tcgaactgga agtcatcggg gccgatggtg
    18721 ccgtcgaagt cgatatcgcg ccactgcagg atggagcgca taccgaccag gagggcgagg
    18781 tcgagatcga tgtcgcgcgg gccgaagttc ccgtgcgtct tgccgtccca gatcggccgg
    18841 agatcgtcga cggcggtgcg gacggcgccg aagaggcggt tggccttctc gtccggccag
    18901 ttgtcgccga agaacggcag caggtcgttg ctggcgaatt catcttcttc gagttcgctt
    18961 ccgagccgga gggcgttgaa gccggaggcg tgaatgacgg ccttggcgtg gtagtagcgg
    19021 gcgtcggaat tctccggatc ttcgtccatc gccttggcga actgctcggc ggccgcggcg
    19081 taatcgccct cgcgcatcag ggcgttgcct tcggcgatcg gatcgtcctc agccgcccag
    19141 tcgaagatgt tgcagccgac cgtcagcaaa ccggcggaga gcacgacagc cagcagaaca
    19201 gggagcctac gatacatggg aacctccagg gacatatagg atgagtaggt ttagttcggg
    19261 ggcggggcgg ggcgccgaag acacaggagc aggcgcgtcg ggacctgcgg gtgaaagcgg
    19321 gcggagtccg cgacgggggc ccttgctagt catcatcagc gtaaatccct gcgtttcgtc
    19381 cggcctggtc tggttgattg ctcagcgacc ccggtgcaac ggactcggcg ggtcgccggt
    19441 cacgcgatcg atggatgaaa tcttccttca gcgggcacgg gcaaccggcg caacgaaggg
    19501 gcagaaaacg gaagccgccg cctgctcgtg ccagaccctg acacccctta acataccgta
    19561 tgaagtcgtt gatgtcaagg gaattatggc ggccccgccg gtggacgagc gccgggaagg
    19621 ggtcaaaaca aaaccgccct cgggggaggg cggttgggga agatcggttg ccgaagcgcc
    19681 gtcagcgcgg ctgcggtttt gaccaggccg ggttcttgtc caggccgggc ggggtcagac
    19741 ggcgcgcgcc gttgcccgtg atgtcgcaga tccacaccga cccgtccgac gtggcgaatg
    19801 tcagccactt cgagtcgggc gagaacgagg ggggattggt gtagagcatg attccgacgg
    19861 gcggcgagaa gacggtgaag tgctctttca ggtcgggcgt ggcgatgcag acctggggag
    19921 cgcttatatc gtccaggatg taagccaacc actggtcatc gggcgaccag gagggggcga
    19981 tgcagcgcag gtttttcgcc gaggcggcgc ggatggaatc gatggagatc atcgggttgg
    20041 cccggtcaaa caccaccagc cccttcccct taaggtcgga catgtggacg caggcgattt
    20101 gccccttgcg gttggcggtg gggaaggtga ggaagtcgtt gctgaagttc tgccagtcct
    20161 tgcgcaggat gttgaacttc ccgccgtccg gggacatggt gcagatctgg aagttgggga
    20221 ggaaggcgtt ggcgtagttg gcgttcatgt ctttccagaa gacgatctcg gaaccgtcga
    20281 gcgaccattc cgggccgcgg ctgccgaggt cctcgctgag ccgcgtccac cagagcgtgt
    20341 tgttggcata ggccgagtcg agccaggcga tgaagatgtc gtagaccttg tggagaccgc
    20401 cgacattggg gtcgggcccg cgcaggtcga tcttgccgga gcggacgaaa gcgatgcggc
    20461 gattgtccgg ggcccacgac aggctcccgt cggcgttgcc cacctgcgag accagttgct
    20521 ggcccgagcc gtcggcgttc atgatccaga cgttgccgtt gcggaggaag gcgatctgcc
    20581 cggtcggggg atcgatcttc ccggcgcggg cggggggaag tttctccgcg gcggccgcac
    20641 tcgcggcgag agccaggccc gccacgagcg tcgccagcag gcgcgcggtc cggttgtcag
    20701 tacttctcaa gatacgtctc tccatctgag gttggcacag ttcggtgcaa taacagggat
    20761 cgcgccgaac ttgtcaacct ccgcggcgcc ttccgactca gaaggcaaag taccgtttca
    20821 gttccacctt gccccggaag ccggagaggt cgagatcgac gcggccatcc tggtcaccgg
    20881 tataggtgac gatgatctcc tcgtcttgag cattatacca ggcaatgttg gtgaagttca
    20941 ggtagaggta cccgaagtcg gcgccgaggc tgaggttgag gacgttgagc gggatctcac
    21001 cgccgaggcc cagggtgaaa ccgggggcgt tgccctcaaa ggtcgaattc tcgctctcgg
    21061 aagccgcggt ggagaggttg aggttctgat actcgggcca gagttcccag gacgcgtggt
    21121 agtacccgac ggcgccgaag gcgcgggcgg acaggccggc ggccggccga tcgggagcgg
    21181 gcgcattgag gaggaagtac tgcagcccgg tggtcacgcc gtagactttg atttcgctgg
    21241 tgaggttatc gaccaccgtg gcgccgccgc tgggagggga ataggtgagg gggccggcga
    21301 tatcggagcc gaatttcagc cagtattctc cgccgaccgt ccatgcgagc cggggcttga
    21361 cgcccacggc gaagtcgacg ccgaaagtgg acgcctggcc cagccagggg agatcggcct
    21421 ccgcaatctg ggcggagatg gagctggcga aactgttgta gtcgttgttg cggttgatac
    21481 gattcagggt gtagttgagc gccacccagc cgattttctg cacgtgcttc ttctcaactt
    21541 ttttcatgta gcggttgacg atctcctgct cggtttccgc cgagagggtt ccggccgcag
    21601 acactaggag cgcaaaggcg ctcaccaggc acacgaaacg acgcatggca acctcctgtg
    21661 aactggtaag aatcagtttg tgattttatc ggaaccgctt tcgggggact tgacggaaga
    21721 gcgtgtcttc gaaggcgttc gcgcaaaaat aggacgggcg gaggcggggc gcgggattgt
    21781 tcagattgtg cacagcggag cggggcggcc gggccggagc ccaaagaaaa cggggctcgc
    21841 gaagagcccc gtggaaatgg cacagaaaac ggccggtcgg gcggctagtc cccggccctg
    21901 ggcggcaggg agtagtcgag gccggccagt tgggagtaca gccgccagcg gcggtcacag
    21961 tcctgctggc cgagtttcag gagcatggcg gcccgctccg ggtcgatggc tttgagcgac
    22021 cggaagcgga tctcgctgta ggcatactcg gagaagggga tggacggcgg cttgctgtcg
    22081 agctggagcg ggttcttccc ctgctcgatg agatccgggt tgtagcggta gagcatccag
    22141 tggccgcagc ggacggcctt gtcttcctgg tccatggagt gggccatgtc gatgccgtgg
    22201 gcgatgcagg cgctgtaggc gatgatgagc gagggaccgt tgtagcgctc ggcctcgatc
    22261 atggctttga cggtctggcc gttgctggcg ccgagggcga cctgggccac atagacgctg
    22321 ccgtacgaca tcatcatcat gccgaggtcc ttcttgccgc gcggcttgcc ggcggcggcg
    22381 aacttggcca ccgaaccccg cggcgtcgac ttcgacatct ggccgccggt gttggagtag
    22441 acctcggtgt cgaggacgag aaggttgacg ttcttgccgg aggccaggac gtggtcgaga
    22501 ccgccgtagc cgatgtcgta cgcccagccg tcgccgccga tcacccagac cgatttcttg
    22561 acgaggtagt aggcgatgct ccgcagggtc gcgaccttgt ccgagtcccc gagggagtcg
    22621 agccgcgcca gaagggcggc gacgcgttcg cgctgctgcg cgatgccggc ctgctccgac
    22681 tggtcggccg aggcgagggc gtcgtacatg tcggggacga gttcccggac gagggtgagg
    22741 gcgtagtcct ggagcttgtc ggcggtgagg cgcatgccga gtccgaactc ggcgttgtcc
    22801 tcgaacaggg agttggacca ggccggtccg cggccgtcgg cccgccggca gtacggagtc
    22861 gtggggaggt tgccgccgta gatcgaggag cagccggtgg cgttggcgca gatggcgcgg
    22921 tcgccgaaga gctgggtcat cagtttgagg tagggggtct cgccgcagcc cgcgcaggcg
    22981 ccggagaact cgaagagcgg acggatgaac tggctgccct tgaccgagtc gcgtttgtag
    23041 agcgacgggt cggtttcggg aatggagagg aagaaggcga agcagttggc gcgctcgcgc
    23101 tcgcggatcg gcgcctggag cgtcatgttg atcgccttgc ggccggtctt ggtcccctgc
    23161 gcgtctttgg cctcgaccgg gcaggcgttg acgcagacct cgcagccggt gcagtcttcg
    23221 ggcgccacct ggagggtgta gtacttcccg gcgagctgct tggttttggc ctcgacgaag
    23281 gggaactcgg ccggcgcgcc ggcgaggtct ttcttctcgt acactttcgg gcggatggcc
    23341 gcgtgcgggc agacgatcga gcagatgttg cactgcgtgc agatttccct gtcccagatc
    23401 gggatgtcga ccgcgatgtt ccgcttctcg tactgggtgg tcgccgtcat gtaggtgccg
    23461 tcatcgggga aggccgagac cggcagatcg tcgccgcggc cggcgatgat ttcggcggtc
    23521 acgttcttga caaactcggg ggcatgggcg gggacgatgg gcggccgggt gatggtcgat
    23581 gtgaccttgt gcggatactt gacttcgcgg agggcgtcgg tggcgccgtc gaccgccgcg
    23641 aaattcatct tgacgacctt ttctcccttg gcgccgtagg tcttgacgat cgcgtgcttg
    23701 atggccgtga tcgcctcgtc gcgggggagg atgtcggaga tggcgaagaa ggcggtctgc
    23761 atgatcatgt tgatgcgggc gccgaggccg agttccttgg ccaggctgat ggcgtcgatg
    23821 acgaagaacc gcgccttctt gtcaatcagc tgttgctgga cctcgcgggg catccggtcc
    23881 cagacctcgt cggggccgta gggcgcgttg agcaggaaag tgccgcccgg cagcagcggc
    23941 gtaagcatgt cgtacttctc gaggaaggag aagttgtggc aggcgacgaa ttgggcggag
    24001 cggatgaggt agggcttgcg gatgaggttc ttgccgaacc ggacgtgcga gaccgtgatc
    24061 gcgcccgact tcttggagtc gtacacgaag tagccctggg cgtagttgtc cgtgttttcg
    24121 ccgaggatct tgatcgagtt cttgttggcg ccgacggtgc cgtccgcccc caggccgtag
    24181 aacaggccgc ggaaggcctc cccctcgagg tcgaacgagt agtcgggttc gagggaggtg
    24241 tgggtgacgt cgtcgacgat gccgacggtg aagtggtcct tgggccgggc ggcggcgagg
    24301 ttgtcgaaga ccgccttggc catcggcgga ttgaattcct tcgacccgag cccgtagcgg
    24361 ccgccgatga cgagaggcgg ctgggagaag ggggcgatcc ccttgtcgag cgcctcgcgg
    24421 atcgccgtct ggacatcggt gtacatcggt tcgcccaccg agcccggttc tttcgtgcgg
    24481 tcgaggacgg cgatgcgccg gacggttttc gggagggcct gcacgaagtg cgcgaccgag
    24541 aagggacggt agaggcgcac cttgatgacg ccgactttct cgccccgggc cgcgagggcg
    24601 tcgacggttt cgtggacgac ctcggcgccg ctgcccatga cgaccacgac gcggtcggcc
    24661 tccggatggc ccatgtagtc aaagaggtgg tagccgcggc cgacgatccc cgcgaagcgg
    24721 tccatggctt tctggaccag ggcgggggcc gcgaggtagt accggttcac cgtctcgcgg
    24781 tgctggaaga agacgtcggg attctgcgaa gttcccttga tggtcggatt gtcgggggag
    24841 agagcgcggc gccggtgggc gacgatcagg tcgtggtcga gcatcgccgc catgtcgtcg
    24901 atagtgatct cctcgacctt ctgcacttcg tgggaggtgc ggaagccgtc gaagaagtgg
    24961 acgaagggga cgcgggaccc gagggaggcg gcctgggtga tgagggcgag gtccatgacc
    25021 tcctggaccg acccggaggc catgaggccg aaaccggtcg agcggcaggc catgacgtcg
    25081 gaatggtcgc cgaagatcga gagggcgtgg gaggcgacgg cgcgggcgga gacatggaag
    25141 accgtcgggg tcagctcccc cgcgatcttg aacatgttcg ggatcatcag gaggaggccc
    25201 tgggaggccg tgaaggtcgt ggtcagggcg ccggtggtga gggcgccgtg gacggcgccg
    25261 gaggcgcctc cctcggactg catctcgatc acctcgggga tgacgcccca gatgttcttt
    25321 tccccgcggg ccgacttctc gtcggcgatc tcgcccatgt tcgacgaggg tgtgatcggg
    25381 tatatggcga tgacttcgtt ggtcgcgtgg gcaacgtatg ccgcggcggt attgccgtca
    25441 atcgtcacca tgcgccgccg gtgcggctgc ggtttcggca cgcgcttttc catcgatgaa
    25501 acagcttctt gcggcattct gagactcctg taggtagcag cttgcatttc cccgacggcg
    25561 actatcggct gtcgtcggcg cgggaaaccg tgatatcttt ctgagcctgg tattttccgc
    25621 tcttgtcccg gtacgaccgc aggcagtcct ggtcgccctg gaggaagagg atttgggcga
    25681 tgccctcgcc cgcgtagacg cgcaggggca cgggcgccgt gttggccagc gagagcgtgg
    25741 cgtacccctc ccactccggt tcaaaggggg tcacgttgac gacgagcccg gagcgggcgt
    25801 aggtggattt gccggtgcag agggtgatga tgttccgggg gatgcggaag tattccaggg
    25861 accgggccag gatgaaggaa ttggccggga tcgccaaaga ttcggcccgg gcgtccatcc
    25921 agcagtcggg gcggacgttt ttcggatcga gctcggccac gcgggtcgtg tcgaggagct
    25981 tgaactcgtc ggccaggcgg aagtcgtagc cgtagcagga gacgccgaaa ctgatgccgg
    26041 cccggatctg atcgcgggcg aagggctcga tcatccggcg ccgttcggcc atttcaatta
    26101 tccagcggtc cgccatcacc gccatagcac gcgcgactcc cttgttattt tcttcacaaa
    26161 cctcaagata cagcgcgggt ggcgggatgt aaaccggaaa ctacataaaa atcacctgtc
    26221 gggacacgct agatagtggc ggccttgttc ttctcccacg agaggagatc ggccagagaa
    26281 tgttcttcga acacggagac gagacgatct tcggccgccc ggaggagttc gcggagggcg
    26341 cagaaatcgg ttttctcgca gatggcgttg tctttgatgc acttaatgag gtggtagggc
    26401 ccctggatgg cttccagaat ctccttgacg gagatgtcgg ccgggccttt ggccagcgtg
    26461 aagccgcccc gcacgccacg gtgcgagcgc acgatcccgg cccgggacaa cgactggaaa
    26521 atcttcgcga gaaacttctc aggaatggcc cgtgcctccg agatttccga cagcggtgtc
    26581 acggtcgcgt cgttgcgctc ggccaggtac aaaacgccga ggatgccgta ttcttcagct
    26641 ttagtgaact gcatgactaa ccttctttcg ctgcgctttt cggagtctgc ctgtcatgag
    26701 atgtcagaag gcgacaagtg ttaccgaatt tgttttctac atgatacgca acatagacaa
    26761 gggcgtcaat aaaaaaaggc aaaaacgcgg ataaaaatgc cgattttgtc ctccgaatgc
    26821 acagtgatga cagccgttga ctttgagcgg caatgtagta ctttgcggct aagcggatta
    26881 caccggcaca aggacaggaa ggttatggcg gaaaccgaac gcgagaagaa gaatacgggg
    26941 cttttgatcg tctacacagg caacgggaaa gggaaaacca cagccgccct ggggatgtgc
    27001 gtccgggcgg tgggctacga ctggcaggtg tgcgtgatcc agttcgtcaa ggggagctgg
    27061 aagtacgggg agttgaaggg gatcaagcgg ttggcgccga atgtcgaatt gcacacggtc
    27121 ggagaggggt ttgtcgggat tgtcgacgac accaaagatt tcgaggaaca ccgggcggcg
    27181 gcccggaagg gggtggcgct ggcactggag aagattcgct cccgggcgta tccgctggtc
    27241 atcctggacg agctcaacgt ggcggttgac ctcggcttgg tgaccgatga cgaggtgcgc
    27301 gagctgctgg ccgcccgcga tgcggaacag tccctggtag tgaccgggcg cggggcgcgc
    27361 gactggctgg tggagcaggc ggatttggtc accgagatgc gggagatcaa gcaccccttt
    27421 cagaaggggg tgctggcgca gaaggggatt gactggtgag gggcggcaga gctaattggt
    27481 gagccgaacc ggcgccgcgc cgggagtgtt tcagcaggtg cggccgcccg gcgcggccga
    27541 agaggaagaa ccctggacga ggagtctcat gtctacagcc gagaagaact acgatatcgt
    27601 cgttgtcggc gccggtcccg gcggcctgtg cgcggggctc tacggggcgc gggcgcggag
    27661 aaaggtcata tgcctggaga agtacatgcc ggggggccag atcgccaaca ccgaagaggt
    27721 ggaggattac ctcggattcg tccgcatctc cggccacgag ttggccacgc gcatggcgga
    27781 gcacgccatg aagttcggcc tggaggtcgg ctcggaagag gtggaggaga tctatgtgga
    27841 cggtgatgat cgggtggtgc agtgcgcctc gggcacacgc taccgggcca aagcggtgat
    27901 cctggccacg ggcggttcgc cggtgaaact cggcgtgccg ggcgagcggg agtacgcggg
    27961 gcgcggggtc tcctactgcg cgatctgcga cggagccttt ttccgggacc aggtgatcgc
    28021 cgtggtcggc ggcggcgacg ccgcggtcga ggagggggcg tttctcacca agttcggttc
    28081 caaagtccac atcatccatc gccgaaaaga gctgcgggcg cagaagatca tccagcagcg
    28141 ggcgaaagac aaccctaaga tcgagtttgt cctcgaaacg gtggtggagt cgatcaacgg
    28201 cgatggccgc aagatcagcg acctgacgct gcgcaatgtg acgaccggag aggtctcgac
    28261 gcttccggtc ggtgcctgtt tcatcttcgt gggtttccgg ccgaattcgg gcctcacgcg
    28321 cgaggagctg cgcaaagacg ccggcgggta catcatcacg gacgacaaga tggagacgtc
    28381 gatcccgggg atttttgcct gcggcgatgt gcgggcgcag ttggtgcggc aaatcaccaa
    28441 cgcggtgggg gatgggacga cggcggcggt ggcggccgag aagtacatcg agcacctcga
    28501 ggacaagcgg acggcgcaat cggcctcccg ctgacacggg ggccgttcgg cgggtaaagc
    28561 gcggttgcgg ccgcaggcgt cggccgagcg gggcggacgc acgccggcga gcgaccgcgg
    28621 ccccagcctg aggcggccga ccggcgggaa ctaagtaact cccctgcggg tgtaagtcgt
    28681 atcctgacac gccatggaaa ccgggggcgg tgcggattcg gacggtgcag agatcgcgtc
    28741 ggccgggagc tgttcgtttt tcgccggccg gccgatagag caatcagatt ctgggagtgg
    28801 cgtgtgctgg actttccagc cacctgacgt tagagtcggg catccccctc cgccactccc
    28861 agttttttta tgcctgcccc ggccgttcgg ccggctaata ccatcgggag gtcggccgat
    28921 aacaggaggt atggaggcaa gtcgcgagtc tttgctcgat cggctgacgg cgggttggaa
    28981 gccgggcctg atgtcgcgct tgagccacct gattatcgtg caggtggttt ttgtgtttgc
    29041 cgcgctggtg ctgattatat tctcccccgg ccactacttg tcagggcggg cggaaacgcc
    29101 gcgccttccg gatttcgcgc cggtcgcagc ggcggcggcc gccgaggcgg cgggccggat
    29161 gccggccgac taccgcaccg agggagcggg cgggcaggct ctcgccgcgc ttctcggcgg
    29221 ccatacggcg gtccggcagg cggtgctcta cgagctggcg gcgggtcgcc cggcggccct
    29281 gtggggctac ggacgggagg aggactcgcc gctgccgccg tcgtggacgg accacgaact
    29341 gacccatctc cggctgagcg cggccgacac ggcgggcgcg gacacccacg gctggccgac
    29401 cgtgccgctc cgtttgacgg agcagttcat cgtctacggg gcgccggtcg agttcaggga
    29461 cggatcgagc gggctgctgg tcgcggcctg ggaccacaac ctgttcgtgt cggaccggag
    29521 ggcgcttggc tacgcgctgc tcgtgctgtt tctgtgcgcc ggcctggtgg cgctgttgac
    29581 ggtgtatctc atccacaacc agttcacgca gccgttccgg cggatgatcc accggttcga
    29641 gcggacctcc gacggcgagc tgtaccacat gctggagaac tgcggggagg gggagctcaa
    29701 ccggctggcg gcggtgttca acaagttttc tcgcacgctg ctggccgacc agcgggagct
    29761 cagccggtat gacgcgcagt tgcgcgacgc gagcagttcg ctggaggatt cgcagcggct
    29821 gctggcggcg gtgatcgacc actcgccggt cggcgtgatc acgaccgatg cgggcggccg
    29881 catcctgctg ttcaatcgcc gggccggcgc gatattcggc taccgcgcgg ccgaggcgct
    29941 cggccgcccg atcgccgacc tgttcgtcaa cgacgtgcag gcgcaggcgc aggcggaggg
    30001 ggaatacgag gacgagcacg gcctggaggt ggtggggcgg cggcgtgacc gctcgcagtt
    30061 tgccgcctac ctggtggcct cggccctgcg caccgacgcg ggagcgggca tcggctacgt
    30121 ctacatcgtc cgcgacatct cgcagcggaa agatttccag gccatgattg tgcggctcga
    30181 ccggtactac acgcgggggg agatggccag cgagattgcg cacgagatca acaactacct
    30241 ctcgatcctc atgggcaata tcgagctcgt gccgctcctg ctgcgcaagg ggaataccga
    30301 gcggctggag cagaagctcg aggtgatgca cgggacggtg gagaagattg cgcggttcac
    30361 cgacgggttg ctggacggga gccacgacca gatccagttc gcgcggtgcg acatcaacca
    30421 gctcgtgcag gcggtcgtgg cgttcctgca gccgcagaac cggttcgaca acaccgcaat
    30481 cgtgtgcgaa ctgtcgcccg acatccccct ggtggaggcc gacccggggc tcctccagca
    30541 gttgctggtg aacctcgtgt acaacgccgg cgacgccgtt ctcgggagcg agggtccgcg
    30601 ccggatcgag atccggacgt tcccggcggc gggtgcgggg gcgccgacgg tagggatcac
    30661 ggtgcgcgac acggggccgg gggtggcgcc ggacaagctg ccactgctgt tcaagcggcg
    30721 ctttaccaca aagccgcgcg ggcacgggat cggcctgatt gcgtgccgga aaatcctcga
    30781 ctcccacggc ggcgacatcg cctacgcgca cgacggcggc gccgtgttca gcgtgacgct
    30841 gccgatcgag caccgttccc gggagtccgc agcctccccc gccgcggcgg cggcggatat
    30901 tccccaagtc tgatttttgt tgccgcgccg gggggccgcg ggtagaatca acgtgatggc
    30961 cgagcgtgcc aacgggaagc ccacctgggt ggagcgccgg attccggcga tcggggcgat
    31021 tgtgctggca gtcgcggtgg gcgtgtacgc ctggatcggg atctcggaga gccgcaaaga
    31081 cagtctcggc ctgctggtga cgcagggggc ggccttcacc gaggcgctgg ccttcgtgtt
    31141 tcaggcccag gacatggagc tgc
//
LOCUS       DAOPOE010000029        31066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1900_length_31066_cov_5.413015, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000029 DAOPOE010000000
VERSION     DAOPOE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31066
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_31066_cov_5.413015"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKM94_05615"
     CDS             <1..428
                     /locus_tag="PKM94_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66153.1"
                     /translation="GLRLVVELHLDLAAVRAGWEGFLAELRQRKAMLSSQLAMATLVE
                     VKDNELAVVFGAGGTMPLQIVTRTENLHVITTALREHYRAPLRIKFSLDPRRQGPGPT
                     EEKRGLDRAQIEKLIADSPRIRRLLDLVEGEVIGVRKVT"
     gene            453..779
                     /locus_tag="PKM94_05620"
     CDS             453..779
                     /locus_tag="PKM94_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOD66154.1"
                     /translation="MRGGLGDMMKQVQQMQAKMAALQEELENTELEASSGGGMVKVVM
                     NGKNVVRSITIDREVVDPEDVEMLQDLIVAAVNQAREKVQELQAQQMAQITGGLNLPG
                     LGGLPF"
     gene            802..1398
                     /gene="recR"
                     /locus_tag="PKM94_05625"
     CDS             802..1398
                     /gene="recR"
                     /locus_tag="PKM94_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD66155.1"
                     /translation="MFKSAASVERLTNRLARLPGIGRKSAARLAFHLLKLPREEALEL
                     ADAIREVKDKVGFCSVCNNISETDPCQICSDPRRRRSLICVVEETADAAAIDRVEGFD
                     GVFHILGGRLSPLDGIGPDDLKIKELLARIGGGDTEVVIATNPNVEGEATAMYLSRLL
                     KPMGVRVTRIARGLPVGSDLEYADAVTLARALDGRQEF"
     gene            1352..3442
                     /locus_tag="PKM94_05630"
     CDS             1352..3442
                     /locus_tag="PKM94_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66156.1"
                     /translation="MPSRSHGRSMGGRNSDWPAMHRTGTHSQLGLLAGLTVLLFVAGR
                     LASGALGPNNWSFTHWQALARPSAAAWLAVALVLGVALGRFLPAIAAFLQPRRKAAVG
                     ATLLIVLIVLLRCDSFVAGGGNLRIAQIAQTADVILPWYEYGAVAAVRLFYRCGVIAG
                     LPEAAAAAAAWQVFGFACTILALAAAWRLARLLSDDPTRRLGLFVIVFFGPQTAVYFG
                     FIGPVPVIAAAALWLAYALVRAAEKPSPRRLGLVWGVFALSVAMHAAMVFALPAVVAG
                     TVGGLRRRGRPGVAPFLAGAVVWIGLPAALYLVANEYLPLKASLLLAAGKPPHGDYSL
                     FSPRRLGDLGQVLFLLFPLGLVALYALIRWTRRAVTDLACSTALLLFLGGLSAVFVID
                     PVHSIVLDLPLYAPFLAPGAVFLAVSLRATSGSPSISPSALGMIAAASLMLPAGYLPV
                     YTRLATVERYITPYLDRHSAYYRTAAVALRDAYFYRKDLERANYWDQSYRRKSPDYLN
                     LTGIEDFILAQRLDIALPSLNKLIARNPYWAEPRAVYGKVMTEQRRFDLGKPQLDTAL
                     MLEPYNPRRHMNLYGYYRDKGEFAQALTAVTRAAELFPNNDEIRTDLMIISYRAGHGE
                     RARELAEHLLRLDSTRAYPYAIMGFLAEEAGDLSSAMANFERFCSLAPEAPETPQIRK
                     RANAIYLKLNPAAP"
     gene            3574..4035
                     /locus_tag="PKM94_05635"
     CDS             3574..4035
                     /locus_tag="PKM94_05635"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOD66157.1"
                     /translation="MQKSEQPGRFRYAGLGRRFAALMLDFALGSLVFFPVTKIAKGVW
                     ILRPEEHAWRYGWLITDPLCLMFLAVILAYFILSEGLLGATLGKRLVAIRVVRVNGER
                     PGLGRSLIRNVLRAVDALPAFNILGIVLIVSSPEKARAGDRVAGTRVIALR"
     gene            complement(4083..5477)
                     /gene="sthA"
                     /locus_tag="PKM94_05640"
     CDS             complement(4083..5477)
                     /gene="sthA"
                     /locus_tag="PKM94_05640"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HOD66158.1"
                     /translation="MREFDLVVIGSGPAGEKAAIEAAKMRRSTAIVERRSVQGGVCIH
                     TGTIPSKTLRETVLYISGLRQRSVYGLVGGVRANVSVAELMYRKGQVIQQEVDIIQQN
                     MARHRIEVIQGTGRLRDAHTVEVVNDRGAQIELRGEVIVISTGARSWHAPNIEFDHKH
                     IYDAESIINIDELPRSLTIIGGGVIGCEYASIFGHIGVNVTIVDSRERLLSFLDREIA
                     DALAYLMRKYRINLVLGDGMKEVFRRDGQVVTVTQSGRRVAADRLLFTAGRVGNTQNL
                     GLENLGIEVDDRGLIRVDSLYRTRVPNIYAVGDVIGFPSLASVSMDQGRLAAIHAFNH
                     GDTSCINSLLPFGIYTIPEVSMVGQTEEALSAEGAEYEVGMARYYELARGQIINDHDG
                     MLKLIFDPRSRRVLGVHIVGERATELIHIGQAVMTFGGTIDYFTDTVFNYPTLSEAYK
                     VAALDGFVRLSARG"
     gene            complement(5480..6541)
                     /gene="rmuC"
                     /locus_tag="PKM94_05645"
     CDS             complement(5480..6541)
                     /gene="rmuC"
                     /locus_tag="PKM94_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOD66159.1"
                     /translation="MDPALVLLVVLTAMLTAAVVGLAVSLRRRQTAETQTVQAAVDRL
                     RAELVGAHLEGLATLRSSLDAAARGLNERLAEGTSAMDRRLTVVAEIEHRLGELQRQT
                     QNLEDIGRNIQALSDLLKPPKLRGGLGELLLENLLGQILPPALYAMQHGFRDGSRVDA
                     VIRLGDRFLPVDSKFPLDAFARLSAESAGEAAAREFQRAVKVQIDAIAAKYIRPDEQT
                     TDFAVMYVPSEAVYARLISDEGGGLLPYALARRVIPSSPGHLYAFLASVAAIYREAGL
                     AGNTRRLSVVVSGVRAALERLDRTHERMAGSTRMLALNLDKARAEAGAMKELLDRVHG
                     PDAAEPAEDNPAAGEGRIK"
     gene            complement(6553..8139)
                     /locus_tag="PKM94_05650"
     CDS             complement(6553..8139)
                     /locus_tag="PKM94_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66160.1"
                     /translation="MKGRSFVLAALLVAFRPAVSPAAEPFSTRPAIETLEEYFDLLVS
                     GNLASAEGFWTQAAIERSGRFDITFTDIPLKIDAGSPVVRNLPVMRDYLVPAVTQVDL
                     LDGGRYVRMKFKKTVNGEEVEHPYYAYWDSTYFWLTYPQDWVAALWPVRETRYFRIHT
                     QPGAEAYLHPVLLDEADRFIERAADSLSLGAAVLREIAEKKIEFFYCTSDSTVLTITG
                     HLTKGTYDLASNDIISATFPHNHEVVHLLVNLKLRSLPLMTQPIVREGIAVHLGGRWG
                     KAPTSLVYLGAFLHKNGLVQVDSLVSMDRFEKQAGADMAYPVAGLFTAFLLDRMGYSR
                     YFDLYLKLSGSSTEMPAMLAPEVKRVVYETVGVSSWDNLMMEFSAYCDRLESERAVFA
                     PGLVERKQGGFDDGGVKVRLYQRWVSLSFPANGPDGPRGNLLFGYDKRLKDVKSVLFE
                     EQYQGGTPFPGYRWGIRYDANEAGLYDYATNQLVGKYIYGITPSAAYRGTAQEVQVRF
                     LIDLSEGLAPTAKDHKLLAD"
     gene            complement(8284..9258)
                     /locus_tag="PKM94_05655"
     CDS             complement(8284..9258)
                     /locus_tag="PKM94_05655"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD66161.1"
                     /translation="MKKTLCIIALLLIAGNAFAEFSKVGSAGAQFLKIGVGSRYQAMG
                     DASVACVSDVYAAYWNPAGLAEIDNSAVSFTNVNWVLDVNLNYAALAHYFEDVGTFAV
                     SATVLSMDDQEITTLEEYEGTGDYYSASSYAVGLSYARMLTNRFSFGATVKYVGERIH
                     HENANGFAFDMGTQLYTGFKSLRLGMSISNLGPEMEFTGSDIDFREDDGTDASYITTQ
                     YDLPMVFRFGLAYDLETGPNSVLTLSSELKHPNDNIQQGALGAEWGFRNQYFLRGGYK
                     INYEEEGLTFGGGLQTAMGSETRLVIDYAWQDFGRLDNTHRFSVGFTF"
     gene            complement(9271..11997)
                     /locus_tag="PKM94_05660"
     CDS             complement(9271..11997)
                     /locus_tag="PKM94_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD66162.1"
                     /translation="MKTFYHTVTAGCLISVSALCLLAAAAGAMAPPQPRPGAPVQIQQ
                     LSPHPATWDQITHNVGNIATTVDNWGYIGGYWYYGLPSGEWPRNSGRNYLGELMLWMG
                     GITPTGDTLVANTEDDFQALPNPDFADNPYKILLSTDTARYYLYDPTDTVGAGEGNPA
                     RGWRVWDSEADQWDYNEVYNSLSASFRPGGPTSMQDSHLRFTDNAGGSSLMGLEVTQT
                     IYQWNYCYNEDFLFVSYDITNASTTDYHDFAFGLYIDIDVGGPDGTGENGRLQDVVAF
                     DQSENLAWIYDVVGVDPGWGPLVRTGIMGTKLLETPDNIGMTAFRTDDWAYLPDNDAG
                     RYAMINSAQFDASLPPTDQFYVQCTRGIDLTAGKTVRVVFALVAGQDEADFRNNAELA
                     QQLYDNNFVGPEPPATPELTGKAGSRKAYLSWSDTSEVGLDPMSGIADFVGYKLYRSD
                     DRGQTWGVQIEDERQLFSNCLSKDYYPVAQYVVNAPGDPITRSFVDTGLVNGVEYWYC
                     LVAFDRGDSVLGVDPLQNGWGIAGEVSNVIKVVPTASPAGYYTAQSTLLHTYSGAEQP
                     SEGAVYADVFNAHARSGEQYAVVFVDSVDETYWHLVNETTGDTVLGYQTATDLEPGHF
                     PIVEGLRVVVTNGPRTPRSTAQTAAGANGLNLVAGSFYGPAIPALTGDTNDVFSDAPF
                     RNTYELRYTGDSTRATWMLDGFYGLDNVYWVPFECWNTSRSERVSLAVYDFESDGDWD
                     PYDLLAVVDYPYDSLSSVTPQAFPYAYSWLIGFDETRYNPSEGDVFTMVGAPMNGPDD
                     RFTFKVDDVNASSAAGALKNIKVAPNPFFVRNNYATAVGELQFKNIPDKCTIRIYTLA
                     GDLVKTIEHADGTGEAYWNLLSGNAQQVASGIYLYHVESDYGEFLGRFAVIK"
     gene            complement(12014..14680)
                     /locus_tag="PKM94_05665"
     CDS             complement(12014..14680)
                     /locus_tag="PKM94_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD66163.1"
                     /translation="MRNRLHMIFCVVGCLAVLALPSAAEAGVTGKVSGVVLDSATGEP
                     IVGAIIRVVGTDMGALTDVDGEYFIINLPGGSHDVSVSYMGYEPVTKTGVRVLIDLTT
                     PLDFRMVPAPVELGRQVVVSAANPIVQQDLTATRIIFTADRLEELPNIVSVQSVLRNY
                     PGVVEDREQALHVRGGRAGQVTYFYDGFNIQDPFTATQGIEIIPSALEELSLTSGGYT
                     AEYGEALSGVVSAVSRDGTAQYHGRLKMYEGFTHPYDVDKGDWGGLERIGNRSLAFDL
                     SGPLPGLDPRRYTFFSAGQYLTDPTSLPHNEVTSWTGTGKLTMYPASGWKFKTNVTYY
                     SADGSVYDHRDVNNRSYDFNLDGLPVFEKEAYLVGFSTDYAFNERAVLTGAVNRFHTR
                     TKSAPDHLFDTYWDEWPGYAVDSAGVYNGTIDDENYLNEPDYSDPMQVVGFTVGDDYY
                     PTYRLRETWYDAFSLRFMHQVNKWNQLKTGAEFRQYDINWDFKQFFNDRPYGEKYQSK
                     PTYASFFVQDKMEYDYFIVNLGLRYDFHNADISYNADPYAATPVWKKAETKHGLSPRL
                     GVSFPIAENSKMHFNYGVYYQEPRFQYLYMNYQGDITTGLPLLGNPDLEPERTTSYEL
                     GLDHLLDNRWHLNVTAYYKDIEDLVTARQDGLIAGREVTRITNGDYGSVKGIDLALEV
                     LPNGGCFTGSVSYGYMIATGNGSYALEPYYTYITSTTDTVQPFTEYPLDFDQRHTVTG
                     VLSYRVPSDWDGRLLGQKLPGDWGLTLVGYYGSGLPYTKTDQSGNRVGERNAWRLPAN
                     YKVDLRFNKDFPLRAGSRLSLFVEVDNLFDRRNVVNLYTSTGLPDNDGVDYMGSLSVS
                     QQALDHYDRLYDNDPQNYAPPRTIRTGLQLNF"
     gene            15009..15950
                     /locus_tag="PKM94_05670"
     CDS             15009..15950
                     /locus_tag="PKM94_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD66164.1"
                     /translation="MKWAFAAALAMSCLASGATPGRSETVAVLMSDTLTSTTRTLQGA
                     KKTIEAANPGIELRTFMIGQSPAGDQAVADSVRSCNPRLVLTIGSTATGIAQHHLPQV
                     PIVFAGVMYPVLSGFVQTIQEPGGHITGASLDIPNEVQFRYFKKIIPGIERIGVLYTA
                     NTASLIAPARAVARGMGLELHAIEIRDSREIPQALDSLALTVQGVWSVADPVLFDPQA
                     TRYILLNTLRKNLPFMGFSRYVVESGALFALDFDYKAVGIQAGQIANRVLAGEAPASV
                     SVSAADVIWFHYNEKTAQHIHVTIPDELVAVAKEVYR"
     gene            15947..17617
                     /locus_tag="PKM94_05675"
     CDS             15947..17617
                     /locus_tag="PKM94_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD66165.1"
                     /translation="MSFLERIMSFRLRTKFVVPISLLLIASILTVSGYLIKRQADGFR
                     RELRTSGETMVRILAMNAESGVLFESRYELQELLQILNQFDAVTYAAIYAEAGKPLAS
                     YGMWSESDIVVKRALDRGARADTIGGGFYVKNADGHEFLELNYPVYSRKEILSRETLG
                     MTSGIDQSLGQRYVTEQIGSIRLILSLAKVNLSIAEAQKTAILLTVVILILTILVLTF
                     FVRVVTKPVQMLVAVTDQVSRGDLSRLVEINQLDEIGHLANTFNKMIASLRQSREEIE
                     EYNRNLEEKIIERTIALEEAQAQLIQSEKMSAIGQLAAGVAHELNNPLGGILGYAQFT
                     LEKMKKNAAAAGLTKEAESYIRYLTDIETQARRCKNIVQNLLRFSRSSHTTEFEDVDV
                     NQVIEDTRTFVEHQLLMNQISLTVDLAEDLPLIQANASQLQQVFTNLIINAMHASRSD
                     SQIRIESRYSPAVGEFGGAVELRFIDQGHGIPPEIINKIFEPFFTTKEVGKGTGLGLS
                     VSYGIVKEHGGEIKVDSAVGKGSTFTLIFPVQKHPVDADNSLKSGIGV"
     gene            17645..18016
                     /locus_tag="PKM94_05680"
     CDS             17645..18016
                     /locus_tag="PKM94_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66166.1"
                     /translation="MDESVRRSVLVVDDEEIIRSFLFEVLSEEYDVSLACDGDEAIEQ
                     IRKRRYDVIITDLKMPRVSGEEVVKFACDRDPQSKVIVISGFSSLYTVSQSINNGACA
                     FLSKPFSIKELMRTVTNALCA"
     gene            18027..18395
                     /locus_tag="PKM94_05685"
     CDS             18027..18395
                     /locus_tag="PKM94_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66167.1"
                     /translation="MARILIIDDSEVIRNLLEEYLTDAGYEVETACDGREGIDRALGG
                     DFDVAFCDIHMPHKNGYQVFREVRRRKPGMAFIMTDSLPDQLAEMAQNEGAHCCLTKP
                     FDLNQVRRVLETLLAKARTV"
     gene            18392..20113
                     /locus_tag="PKM94_05690"
     CDS             18392..20113
                     /locus_tag="PKM94_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66168.1"
                     /translation="MSGTETRSINVLVVDDETIVLSLVRDALEDEGYEITTAVGGDEA
                     LQALDSRPVDLLITDIRMPHMDGTELVRRARERNPHIGVIYMTGYANLNSAKDAIKQG
                     AFDYILKPFELTEIRQAVHKAAGKLRERAGQDSGEQLERLSDLNQMLVTVGDRKSLAS
                     VSLRFAMMHFDATQGALLHWDRERTAVETITIHGDQLQETALDSPAVREFIRAIDPAS
                     FREPVIVTSPERHPLAGPAAAPEIRAALFPSWLTEGSAMVVMPINRANILYGIIMIRC
                     SPDATALTGTDTRLLNIAAGQLAVSLENLFLLEETQTAYARLKEMQDETIQLEKLATR
                     GEMSAEIGHELNNFLGVVAGNISLLDHQLKKSGAATAEKYMSAIQTNIEKMKQFTSNL
                     MDLTPIASKKETVYFDQVLAEVIDYLTPQKRFRGVSIDHTVDHESIPFEADSIHIQQL
                     LYNLFNNAADATRGCARREIVSRVEYRPGEPHFRFVIRDTGVGIEPELLAKAFEQKFT
                     TKPDGHGFGLLVCKRIIDAHSGRLHVDSTPGEGTAISIEFPVAGAADSVDATAGQFAI
                     TDKIPVG"
     gene            complement(20235..21251)
                     /locus_tag="PKM94_05695"
     CDS             complement(20235..21251)
                     /locus_tag="PKM94_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66169.1"
                     /translation="MPRPLPLFIRAAAGAVMLAAALAGCGEKDVPVVVDRDILLRYLA
                     TSEDAVDLFRTTGLFPTEEYRIPNDPARYRIVVDSTRRIITLDTEIDTVKKRTWPDGS
                     VEYYRSYHDYGNPFGRLWASEATVDDYFYVRLSRTEDEETATASHVWAITRYGYFLKL
                     GGDDQPYVGWKLWAYNGGAAPTGSMAVRTESGAEFAGDLAGYDQIPYLYVRECLSTSN
                     PALPCSTVTAAQTSRQVFRRLDRLGAVGPGERLVADCRDVSLRTRYRLLSAATAAGEQ
                     VRVMQPAGPGRFVDTLQMPGTAGRLWEFLFMQDFEYVPIPGTQPPDSQLTWTGWCVPY
                     RIVP"
     gene            complement(21255..23300)
                     /gene="uvrB"
                     /locus_tag="PKM94_05700"
     CDS             complement(21255..23300)
                     /gene="uvrB"
                     /locus_tag="PKM94_05700"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOD66170.1"
                     /translation="MAKPTAPTAAGHTPFRLRSQFSPRGDQPQAIAQLLAGLREGRMH
                     QVLLGVTGSGKTFTIANVIAQYGRPALVLSHNKTLAAQLYGELTAFFPDNAVEFFISY
                     YDYYQPEAYLPTTDTYIEKDTSINEDIDRLRLRATASLLERQDVIIVASVSCIYGLGS
                     PVEYKNQYIPLVRGGEYNRDQVIRKLIDIHYNRNDIDFSRGNFRVRGDTVELIPAYHD
                     HAMRIEFFGDEVERITEIDPLTGEVLRERDRIAIYPAKHFITSPERMQEAIRGIREEL
                     AERLEFFRSRNLLLEAQRLEMRTKYDLEMLEEVGYCSGVENYSRHLSGRRPGERPFTL
                     LDFFPDDFLTIIDESHQTIPQVRGMFAGDRSRKEVLVEHGFRLPSALDNRPLVFEEFE
                     RVVLKRVYVSATPADYELTKAEGIVVEQVIRPTGLLDPVITVRPLGTQVDDLLAEARR
                     RAERGERVLVTTLTKRMAEDLTGYMEKLGLRVRYLHSEIDAIDRTGIIRDLRLAEFDV
                     LVGVNLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTAGRAARNKGGEVILYADKV
                     TESMRKAIEETRRRREKQAAYNREHGIDPETIFKTREEILRSTQFADSKTPEEAPRFE
                     KPSSFTRMSREDQLAFMLAAMRKAAENLDFETAIMIRDEINALRGDGRKNPKPKKGK"
     gene            23553..23873
                     /gene="rplU"
                     /locus_tag="PKM94_05705"
     CDS             23553..23873
                     /gene="rplU"
                     /locus_tag="PKM94_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOD66171.1"
                     /translation="MYAVFEVAGFQYSAQEGDVLRVPHQKADPGTSLAIDRILLVRGD
                     GATAIGTPTVADARIDAEVVGEGLADKVLIYKYKRRTKYRRTQGHRQRFTEIRINKIH
                     APGA"
     gene            23940..26363
                     /gene="bamA"
                     /locus_tag="PKM94_05710"
     CDS             23940..26363
                     /gene="bamA"
                     /locus_tag="PKM94_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOD66172.1"
                     /translation="MLSLSAHAQNTAEQFRVVDIQVEGNRVATRSLILGVASLEVGAV
                     LSVTDVSESIRRLYALGIFRDVRIEAEEVTGGIRIFIIVEELPKLAGLAFEGNKKLKD
                     DKLRKEIGLGVGGYISPYLIEKSRAKIFDLFAAEGYFRAKIEPELIYGADSVEAVLQF
                     NIRERDKVKVEQVVVTGSRRVEAGDLVGRMRNRKRGLLRTSDFAQDKYDEDLQKVIDE
                     YHKRGFIDAYLINDSLAIDTARNRMTIYLDVYEGPRYYFGETSFKGNERLSADHLAKL
                     LKHKAGQVFDSEKYDASLGEVASGYYDIGHLHVEIFDERSTRSDSLIDITYDITEGLP
                     SNINLVKIVGNYKTKDRVIRRELSTLPGQVFDRTALIRSIRDVMALNFFDKVEPRPVN
                     LPSGDVDLVYEVAEKQTGQVSAGAGYNSQDKVVGTFGMGIPNFRGLGQNLSFNVSLGK
                     SYNSFSVSFTEPWMFGYPTSFGASAFSLNRRWYGDYTEGRAGGSLRLGRRLRWPDNYF
                     RVYASYALERTRYYDFDATFAASNSYQSRYYYNNPETISPADSLMAARTYGPYPGSIL
                     EYEDWQTASRVSLTLLRDSRNLPEFATKGSILSYTLELTGGLLGGYWEYTKHDFEAAK
                     FIPLMWGWALAAKAQYGVVTAPAGDARIMISDRFTPGGTAYDGIVRGYDDGSLTPDSA
                     ITTDTIFYYSNENAVIGVDPPDGRYFPLDTTRVRGKYMFVANVEVQIPIAARQLYGLL
                     FFDAGNSWLYRRDIDLLNDLYKSVGFGFRIIVPGIGTLGFDFGYPLDDVEGEPRSWKP
                     HFQIGTTFR"
     gene            26388..26918
                     /locus_tag="PKM94_05715"
     CDS             26388..26918
                     /locus_tag="PKM94_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD66173.1"
                     /translation="MRVTKAASVLSAVLALTVILSGAAAAQTLKIGFVRDDRIYAEYT
                     EWQRAQETWETDRKAWDDEASAKEQELQELTADYEKQRLILSDEKKREREAAIRAKRE
                     GLDAYTKQIYGPGGTAEQREAQLIEPLLTKIRQAMEAVATENSYDVIFTLQGIGYIKE
                     EYDVTDKVLAALNEME"
     gene            26978..27994
                     /gene="lpxD"
                     /locus_tag="PKM94_05720"
     CDS             26978..27994
                     /gene="lpxD"
                     /locus_tag="PKM94_05720"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOD66174.1"
                     /translation="MAFTVAYLAERVGGTVAGDGSVEIGAAAPIETAGPGDISFIANP
                     RYARFLATTRAGAVVVSPQTECPRLPLIRHAQPYLAFACILDLLYPDERLVAPGIDPR
                     AVVAAGAVVDPTAGVGALCHIREGVRIGAGTELVSSVFVGRDVVVGDNCRIYPGVCLM
                     DGTRLGNNVIIHSSTVIGSDGFGFAPSPTGLKKIKQVGWVEIDDDVEIGSNCSVDRGA
                     LGPTRIGRGTKIDNLVQIAHNVEIGRHAIIVSQVGISGSTRVGNGVVLAGQVGLVGHI
                     EVGDGVQVGAQSGVARSVPAGKKVFGSPARDLAETGRIEAALVRLPELLKRVRRLEDK
                     LGGE"
     gene            complement(27998..28576)
                     /locus_tag="PKM94_05725"
     CDS             complement(27998..28576)
                     /locus_tag="PKM94_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66175.1"
                     /translation="MSYASRFIPRTDTPEGQQVATRAMLLIGSAVTLSALIIIAFNWT
                     LSRSTLEQIGSGVELAALTLVPYMIAAAVAAITALCVLLMVPAAQLRQPATVIRDRLQ
                     ALANGDLASRIQGRKPAPHVQELVYELNLATSNLSHQIAQWKVINRQQWELLEAMREI
                     TVVDGHRQLVRIIEQMQANWEKIAEIEERLKT"
     gene            complement(28749..29177)
                     /gene="ssb"
                     /locus_tag="PKM94_05730"
     CDS             complement(28749..29177)
                     /gene="ssb"
                     /locus_tag="PKM94_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD66176.1"
                     /translation="MGVNKAILIGRLGKDPELKYTASGRPVTTFTLATTERWTNQEGQ
                     KQEQTTWHNIVAWGRPAEIMNEYLAKGREVYIEGRIVNRSYDDKDGNKRHISEIVVQN
                     FQFIGSGREGGGQGGGERAPAAEDAAAPEGAAPAEDDLPF"
     gene            29424..30239
                     /locus_tag="PKM94_05735"
     CDS             29424..30239
                     /locus_tag="PKM94_05735"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOD66177.1"
                     /translation="MEARPEIDLDRAVTTITRFLTGKLLQSGLDGYVVGVSGGLDSAV
                     AAALAVAAVGPDKVLGLLLPYRTSSAESLHDARLMVERLGIPHRVIDISPMIDAYYPR
                     ITDELKRRAGNKMARERMAILFDVALETSRLVLGTGNRSEIALGYTTWHGDSACSVNP
                     IGELYKTEVRLLARKLAVPEPIIAKPPTADLWKDQTDEGEIGVTYPQIDRLLARIVDQ
                     GVRSMSQLAAEGFPPTEISRVVSLLNRNAFKRALPEVAPLQRKPIPANVQLSE"
     gene            30250..30960
                     /gene="rsmI"
                     /locus_tag="PKM94_05740"
     CDS             30250..30960
                     /gene="rsmI"
                     /locus_tag="PKM94_05740"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD66178.1"
                     /translation="MPESPGLLYLVPTPIGNMADLTRRAEEMLAAADLVACEDTRHSG
                     RLLKALGLNKRLISYHDFNERTRAEQLIDLIRQGQTVAVITDAGSPGISDPAYRIVRT
                     AIDNGITIIPLPGPTSIIPALTASGLPTDRFFFEGFLPHKSGARRTRLARLTEFDHTL
                     VFFESPHRVVRSLEDIREVLGDRPACLARELSKLHEEFLRGSVSEILAALGGRTVRGE
                     IVLIVGGADSLRQPREEG"
BASE COUNT         5316 a         9896 c        10266 g         5588 t
ORIGIN      
        1 gtggactccg gcttgtcgtc gagcttcacc ttgacctggc cgcagtccgg gccgggtggg
       61 agggcttcct ggccgaactc cggcagcgca aagccatgct ctcgtcgcag ttggccatgg
      121 cgacgctggt cgaggtcaag gacaacgagc tggccgtggt gttcggcgcc ggtggaacca
      181 tgcccttgca gatcgtgacg cggaccgaga accttcacgt gatcacgacc gcgctgcgag
      241 aacactaccg cgcgccgctg cgtataaagt tcagtctcga cccgcggcgc cagggcccgg
      301 gacccacgga agagaagcgc ggactcgacc gcgcgcagat agagaaactg atcgccgact
      361 caccccgcat ccgcaggctc ctcgacctcg tcgaagggga agttatcggc gtgcgaaaag
      421 taacctgagc gacccgaaag gaaagcacca ccatgagagg cggactgggc gacatgatga
      481 agcaggttca gcaaatgcag gccaagatgg ccgcgctgca ggaagagctg gaaaacaccg
      541 aactcgaggc ctcgtcgggc ggcgggatgg tcaaggttgt catgaacggc aagaacgtgg
      601 tccgctcgat caccatcgac cgggaagtgg tcgatcccga ggacgtggag atgctccagg
      661 atctcatcgt ggcggcggtc aaccaggcgc gcgagaaggt gcaggaactg caggcgcagc
      721 agatggcgca gatcaccggc gggctgaatc tcccgggcct cggcggcctg cccttctgag
      781 cgggacggag gccggacacc catgttcaaa tccgcagcat cagttgagcg gctcaccaac
      841 cgcctggccc gccttcccgg gatcggccgg aaatccgcgg cccgcctcgc cttccacctg
      901 ctcaaacttc cccgcgagga ggcgctggag ctcgccgacg ccattcgcga ggtcaaggac
      961 aaggtcgggt tctgctcggt ctgcaacaac atctcggaga ccgatccctg ccagatctgc
     1021 tcggatccgc ggcggcgccg cagcctcatc tgtgtggtcg aagagaccgc cgatgccgcc
     1081 gcgatcgacc gggtcgaggg gttcgacggg gtcttccaca tcctcggcgg acggctctcg
     1141 cctttggacg gtatcgggcc ggacgacctc aagatcaaag agttgctcgc ccgcatcggg
     1201 ggcggggaca cggaagtggt gatcgccacc aacccgaatg tcgaggggga ggcgaccgcc
     1261 atgtacctct cccgcctgct gaagccgatg ggggtgcggg tcacgcggat cgcgcggggg
     1321 ctgccggtcg gctcggacct cgaatatgcc gatgccgtca cgctcgcacg ggcgctcgat
     1381 gggcggcagg aattctgatt ggccggcgat gcaccgcacc gggacacata gtcaactggg
     1441 acttctcgcc ggcctgaccg tgctcctgtt tgtggcgggg cgtctggcga gcggggcgct
     1501 ggggccgaac aactggtcgt ttacgcactg gcaggcgttg gcgcgtccct cggccgccgc
     1561 ctggctcgcg gtcgcgctgg tgctcggcgt ggctctgggc cggttcctgc cggcgatcgc
     1621 cgctttcctc cagccccggc gcaaggccgc ggtcggcgcc acgctgctga tcgtactcat
     1681 cgtccttctc cgctgcgatt cattcgtcgc cggaggggga aacctccgca ttgcccagat
     1741 cgcccagacc gcggacgtga tcctcccgtg gtacgagtac ggcgccgtgg ccgcggtccg
     1801 gctgttctac cggtgcggcg tgattgccgg acttccggag gcggcggccg ccgccgcagc
     1861 ctggcaggtg ttcggtttcg cctgcacaat tctcgcgctc gcggccgcct ggcggctggc
     1921 ccggctgctt tcggacgatc ccacgcgccg cctgggattg ttcgtcatcg tctttttcgg
     1981 tccgcagacg gcggtgtatt tcggcttcat cggcccggtg ccggtgatcg ccgccgccgc
     2041 gctctggctg gcgtatgcgc tggtccgggc ggcggaaaag ccctcccccc gccgactcgg
     2101 cctggtctgg ggcgttttcg ccctgagtgt cgctatgcac gcggcgatgg ttttcgcgct
     2161 cccggccgtg gtcgccggca cggttggcgg gctgcgccga cgggggcggc cgggcgtcgc
     2221 gccgtttctc gccggagcgg tcgtgtggat cgggctgccg gccgcgctct accttgtggc
     2281 gaatgagtac ctcccgctga aagcgtcgct gctgctggcg gcgggcaagc cgccgcacgg
     2341 ggattattcg ctgttctcgc cgcggcgact cggcgacctg ggccaggtgc tgttcctgtt
     2401 gtttccgctg ggtctggtcg ccctgtacgc gctgatccgg tggacgcgcc gggcggtgac
     2461 cgacctcgcc tgttcgaccg cgctcctcct gttcctgggg ggactcagcg ccgttttcgt
     2521 catcgacccg gtgcacagca ttgtgctgga tcttcccctg tacgctccct tcctggcgcc
     2581 gggcgcggtg tttctcgcgg tgagcctgcg ggcgacttcg ggcagtccga gcatctctcc
     2641 atccgcactc gggatgattg ccgccgcctc gctcatgttg ccggccggat acctacccgt
     2701 ctacacccgc ctcgcgacgg tcgagaggta cattaccccc tacctggacc ggcacagcgc
     2761 gtactatcgg acagccgccg tggccctgcg ggacgcgtat ttctatcgga aggatctgga
     2821 gcgcgcgaac tactgggacc agagctaccg gcggaaatcc ccggactacc tcaatctcac
     2881 cggcatcgaa gatttcattc tggcccagcg ccttgacatc gccctcccga gcctgaataa
     2941 actgatcgcc cgcaacccgt actgggcgga accgcgcgcc gtgtacggga aagtgatgac
     3001 cgagcagagg cgtttcgatt tgggcaagcc gcagctcgac acggcgctga tgctcgagcc
     3061 gtacaatccg cggcgccaca tgaacctgta cggctactac cgggacaagg gggagtttgc
     3121 ccaggcgttg acggcggtca cccgcgcggc ggagttgttt ccgaacaacg atgagatccg
     3181 caccgacctg atgatcatca gctaccgcgc cgggcacggc gagcgcgcgc gggagctggc
     3241 cgagcacctg ctgcggctgg actcgacgcg ggcctatccg tacgccatca tgggtttttt
     3301 ggcggaggag gcgggcgacc tgagctcggc catggccaat ttcgagcgct tctgcagtct
     3361 cgcacccgag gctccggaaa cgccccagat ccgcaaacgc gccaacgcga tctatctcaa
     3421 gcttaatccg gccgccccgt gaacccctcc gcaggggcat ggcggctcca cccctgggtc
     3481 ggccgccggg ccgtcgagcg gtacgacgaa acagcacttg gaccgaagcg gtccgggtga
     3541 tagattgccc cggagtctcc cgagggtaac cgcatgcaga agagcgaaca acctggccgc
     3601 tttcgttacg ccggcctggg gcgccgcttc gcggccctca tgctcgactt cgcgttgggc
     3661 agcctggtct tcttccccgt caccaagatc gcgaaggggg tctggatcct gagaccggag
     3721 gaacacgcct ggcgatacgg gtggttgatc accgatcccc tgtgcctaat gtttctcgcg
     3781 gtgattttgg cctacttcat tctctcggag ggcctgctgg gcgcgacgct gggcaagcgg
     3841 ctggtggcca ttcgcgttgt ccgggtgaac ggggagcgac cggggctggg ccgttccctg
     3901 atccgcaatg tcctgcgggc cgtggatgct ctccccgcgt tcaacattct cggcatcgtg
     3961 ctgattgttt cctcgccgga aaaagcgcgt gcgggcgatc gcgtggccgg gacgcgggtg
     4021 atcgctctga ggtaattggg agaggtcgct ctgcgctcag gtgacgccag tccggcggtg
     4081 atctatcccc gcgcgctcaa ccgcacgaaa ccgtcgaggg ccgcgacttt gtacgcctcc
     4141 gagagcgtcg ggtagttgaa caccgtgtcg gtgaaataat cgattgtccc gccgaaagtc
     4201 atgaccgcct gcccgatgtg aatgagttcg gtcgcccgtt cgccgacaat gtgcacgccg
     4261 agcacgcggc gggaacgtgg gtcgaagatg agcttgagca tcccgtcgtg atcgttgatg
     4321 atctgccccc gggcgagctc gtagtagcgc gccatcccca cttcgtactc ggccccctcg
     4381 gccgagagcg cttcctccgt ctgccccacc atcgacacct ccgggatggt gtagatgccg
     4441 aagggaagga gggagttgat gcacgaggtg tcgccgtggt tgaaggcgtg gattgcggcc
     4501 agccgcccct ggtccatcga aaccgaggcg agcgacggga acccaatgac gtcgcccacc
     4561 gcgtaaatgt tcggcacgcg ggtgcggtag aggctgtcga cgcggatcag cccgcggtcg
     4621 tcgacctcga tgccaagatt ctccaggccg agattctgcg tgttgccgac ccgtccggcg
     4681 gtgaacagga ggcggtcggc cgcgacgcgc cgcccgctct gggtcaccgt caccacctgg
     4741 ccgtcccggc gaaacacctc cttcatcccg tcgccgagaa ccaggttgat gcggtatttt
     4801 cgcatgaggt aggcaagggc gtcggcgatc tcgcggtcga ggaacgacag gagccgttcg
     4861 cggctgtcga caatggtgac gttgacgccg atgtggccga agatggaggc gtactcgcag
     4921 ccgatgaccc cgccgccgat gatggtcagg gagcgcggga gttcatcgat gttgatgatg
     4981 ctctcggcgt cgtagatgtg cttgtggtcg aattcgatgt tgggggcgtg ccacgagcgg
     5041 gcgcccgtgg agatcacgat cacttcgccc cgcagttcga tctgtgcgcc gcggtcgttg
     5101 acgacttcga ccgtgtgcgc gtccctgagc cgtcccgtcc cctggatgac ttcgatccgg
     5161 tggcgggcca tgttctgctg gatgatgtcg acttcctgct ggatcacctg ccctttgcgg
     5221 tacatcagtt cggccaccga gacgttggcg cggacgccgc cgacgagtcc gtagacggac
     5281 cgctggcgca ggccggagat gtagagcacg gtttcgcgga gggtcttgga ggggatggtg
     5341 ccggtgtgga tgcagacgcc cccctggacg gagcggcgct cgacgatggc agtggaccgg
     5401 cgcatcttgg ccgcctcaat ggcggctttt tcgccggccg ggccggaccc gataacgacc
     5461 agatcgaatt cgcgcacgat catttgatcc tgccctcccc cgccgccggg ttgtcctcgg
     5521 cgggttccgc cgcatcggga ccgtggaccc ggtcgagcaa ttctttcata gcgcccgctt
     5581 cggcgcgcgc cttgtcgaga ttgagcgcca gcatacgggt cgacccggcc attcgctcgt
     5641 gcgtccggtc gaggcgctcg agcgccgccc ggaccccgct cacaacaacc gacagccgcc
     5701 gggtgttccc tgccaggccg gcctcgcggt agatcgcggc caccgaggcc aagaaggcgt
     5761 agaggtggcc gggggagctg ggaatcaccc gtcgcgccag ggcgtagggc agcaggccgc
     5821 ccccctcgtc ggagatcagc cgggcgtaga ccgcctccga aggaacgtac atgaccgcaa
     5881 aatcggtcgt ctgctcgtcc ggccgaatgt acttggcggc gatggcgtca atctgcactt
     5941 tgacggcgcg ctggaattcg cgggccgcgg cctcgcccgc gctctcggcg gagagacggg
     6001 caaaggcgtc cagcgggaat ttcgaatcca cggggaggaa ccggtcgccg aggcggatga
     6061 cggcgtccac ccggctgccg tcgcgaaacc cgtgctgcat ggcatacagg gcggggggca
     6121 gaatctggcc gagcaggttt tccaggagga gttcgcccag gccgccgcgg agcttgggcg
     6181 gcttgagcag atcggagagc gcctggatat tgcgcccgat gtcctcgagg ttctgcgttt
     6241 gccgctggag ttcgccgagc cggtgctcga tctcggccac caccgtcagc cgccgatcca
     6301 tggccgaggt tccttcggcg agccgctcgt tgagtccgcg cgccgcggcg tcgagactgg
     6361 agcgcagggt cgccagtccc tcgaggtggg cgccgaccag ttcggcccgc agccggtcga
     6421 ccgccgcctg caccgtctgt gtctcggcgg tctgacggcg ccgcagcgat acggccagcc
     6481 cgacgacggc ggccgtcagc atcgccgtca gcaccaccag gaggacgagc gcgggatcca
     6541 tgccgggtcc ggtcagtcgg ccaggagttt gtggtccttg gcggtcggcg cgaggccctc
     6601 ggacaggtcg atcaggaacc tgacctggac ttcctgcgcc gtcccacggt aggccgcgga
     6661 gggggtgatg ccgtagatgt atttcccgac cagttgattg gtggcgtaat cgtagaggcc
     6721 ggcttcgttg gcgtcatagc ggattcccca gcggtagccg ggaaagggcg tcccgccctg
     6781 gtactgctcc tcgaacagca ccgacttgac gtcttttaac cgcttgtcgt agccgaagag
     6841 gagattgccc ctggggccgt cgggcccgtt ggccgggaac gagagcgaaa cccagcgctg
     6901 gtagaggcgc actttgacgc cgccgtcatc gaaccccccc tgtttccttt cgaccagacc
     6961 gggggcgaag acggcccgct ccgattccag ccggtcacag taggccgaga actccatcat
     7021 caggttgtcc caggaactga cgccgaccgt ctcgtagacg acccgtttta cctccggcgc
     7081 gagcatggcc ggcatctcgg tcgacgaccc ggagagtttc aggtacaggt cgaagtagcg
     7141 gctgtagccc atgcggtcca gcaggaaggc ggtgaacagc ccggccaccg ggtaggccat
     7201 gtcggccccg gcctgtttct cgaaacggtc catcgacacg agcgagtcca cctggaccag
     7261 cccgttcttg tgcaggaacg ccccgaggta gacaagcgac gtgggggctt tcccccaccg
     7321 gccgccgagg tgcacggcga tcccctcgcg cacgatcggc tgtgtcatga gcgggagcga
     7381 gcggagcttg aggttgacca gcaggtgcac gacctcatgg ttgtgcggga aggtagcgct
     7441 gatgatgtcg ttggaggcca ggtcgtaggt gcctttggtc aggtgcccgg tgatggtgag
     7501 gacggtggag tccgacgtgc agtagaagaa ctcgattttc ttctcggcga tctcgcgcag
     7561 cacggccgcc ccaaggctga gggaatcggc ggcccgctcg atgaaccggt cggcctcgtc
     7621 cagcagcacc gggtgcaggt aagcctcggc gccgggctgg gtgtggatac ggaagtacct
     7681 ggtctccctt accggccaga gcgccgccac ccagtcctgc gggtaggtca gccagaaata
     7741 ggtgctgtcc cagtaggcat agtaggggtg ttccacctcc tcgccgttga cagtcttctt
     7801 gaatttcatc cgcacgtacc ggccgccgtc gagcaggtcc acctgggtca cggcggggac
     7861 gaggtagtcc cgcataaccg ggagattgcg taccaccggc gagccggcgt cgattttcag
     7921 gggaatatcg gtgaaggtga tatcgaaccg gccggaccgc tcgatggcgg cctgggtcca
     7981 gaatccctcg gccgaggcca ggtttcccga gacgagcagg tcgaaatatt cctcgagcgt
     8041 ttcaatcgcg ggccgggtcg agaacggttc ggcggccggc gacaccgcgg gccggaaggc
     8101 gacaagcagg gcggcgagaa caaacgaacg gcctttcatc gattttctcc tgttctggcg
     8161 cttggtggta cggtccccaa tatacattct ttgtaccgtc tatgtccagc cgggggtgca
     8221 aaaaaagccc cccgccggtc cttgcggggg cggggggcgt aattgccgcg gcggggtccg
     8281 ggctcagaac gtgaacccga ccgagaaccg gtgggtgttg tccagccgcc cgaagtcctg
     8341 ccaggcgtag tcgatcacca gccgggtctc gctgcccatg gccgtctgga ggccgccgcc
     8401 gaaggtcagc ccctcctcct cgtagttgat cttgtacccg ccgcggagga agtactgatt
     8461 gcggaagccc cattcggcgc cgagcgcgcc ctgctggatg ttgtcgttcg ggtgcttcag
     8521 ttccgacgac agggtaagca ccgagttcgg gcccgtctcg aggtcgtagg ccagcccgaa
     8581 ccggaagacc atgggcagat cgtactgggt cgtgatgtac gaggcatcgg ttccgtcgtc
     8641 ctcgcggaag tcgatgtcgg agcccgtgaa ttccatctcc gggccgaggt tgctgatgct
     8701 catgccgagc cgcagcgact tgaacccggt gtacagctgg gtgcccatgt cgaaggcgaa
     8761 cccgttggcg ttctcgtggt ggatgcgctc gccgacgtac ttgaccgtcg cgccgaagga
     8821 gaatcggttg gtcagcatcc gcgcgtagga cagcccgacc gcgtacgagg aggcggagta
     8881 gtagtcgccg gtcccctcgt actcttcgag ggtcgtgatc tcctggtcgt ccatggagag
     8941 gacggtggcg ctcacggcga aggtgccgac gtcctcgaag tagtgggcga gggcggcgta
     9001 gttgagattg acgtcgagca cccagttcac gttggtgaag ctgacggcgc tgttgtcgat
     9061 ctcggcgagg ccggccgggt tccagtaggc ggcgtagacg tcgctgacgc aggccaccga
     9121 ggcgtcgccc atggcctggt accgcgagcc gacgccgatt ttgagaaact gcgcgccggc
     9181 cgatccgacc ttggagaact cggcgaacgc gttccccgcg atcagcagca gggcgataat
     9241 gcaaagcgtt ttcttcacct cgtacctcct ttacttgatc accgcgaatc ggccgaggaa
     9301 ctcaccgtag tcggactcga cgtggtagag atagatcccc gaggccacct gctgggcgtt
     9361 gcccgacaaa agattccagt acgcctcacc agtgccgtcg gcgtgctcga tcgtcttcac
     9421 caggtcgccc gccagggtgt agatgcggat cgtgcactta tcggggatgt tcttgaactg
     9481 cagttcgccc accgcagtcg cgtagttgtt gcgcacgaag aacgggttcg gcgccacctt
     9541 gatgttcttc agcgccccgg cggccgacga cgcgttcacg tcgtccacct tgaaggtgaa
     9601 gcggtcgtcc gggccgttca tgggcgcccc gaccatggta aacacatcgc cttccgacgg
     9661 gttgtaccgg gtctcgtcaa aaccgatcag ccacgagtag gcgtacggga acgcctgcgg
     9721 ggtgaccgac gagagcgagt cgtaggggta gtccaccacg gccagcagat cgtacggatc
     9781 ccagtcgccg tccgactcga aatcgtacac ggcgagagag acgcgctcgc tccggctcgt
     9841 gttccagcac tcgaacggca cccagtagac gttgtccagc ccgtagaatc cgtcgagcat
     9901 ccaggtcgcc cgggtcgaat cgcccgtgta gcggagctcg taggtgttgc ggaagggcgc
     9961 gtcgctgaag acgtcgttcg tgtcgccggt cagggccggg atggccgggc cgtagaacga
    10021 accggccacc aggttcaggc cgttggcccc ggccgcggtc tgcgcggtgc tccgcggcgt
    10081 ccggggaccg ttggtcacca ccacgcggag gccctcgacg attgggaagt ggcccggttc
    10141 gaggtcggtg gcggtctggt agccgagcac ggtgtcgccg gtggtttcgt tgaccaggtg
    10201 ccagtaggtc tcgtcgaccg agtcgacgaa cacgaccgcg tactgctcgc cggagcgcgc
    10261 atgggcgttg aacacgtcgg cgtagacggc cccctccgag ggctgctccg ctccggagta
    10321 cgtatgcagg agggtcgact gggcggtgta gtagccggcg ggactggccg tcggcaccac
    10381 cttgatgacg ttgctcacct cgccggcgat gccccagccg ttctggaggg gatcgacccc
    10441 gaggaccgag tcgccgcggt cgaaggccac caggcagtac cagtactcga cgccgttgac
    10501 gagcccggtg tcgacaaagg agcgcgtgat gggatcgccc ggggcgttga cgacatactg
    10561 ggctacgggg tagtaatcct tgctcaggca gttgctgaac agttggcgtt cgtcctcgat
    10621 ctggacgccc caggtctggc cgcggtcgtc gctccggtac agcttgtagc cgacgaaatc
    10681 ggcgatcccg ctcatcgggt cgaggccgac ttccgaggtg tcggaccagg agaggtaggc
    10741 cttgcggctg cccgctttgc cggtcagctc gggcgtggcc ggcggctcgg ggccgacgaa
    10801 attgttgtcg tacagctgct gggccagttc ggcgttgttg cggaaatccg cctcatcctg
    10861 gccggcgacg agcgcgaaca ctacccgcac cgtcttgccg gcggtcaggt cgataccgcg
    10921 ggtgcactgg acgtagaact ggtcggtcgg cggcagcgag gcatcgaact gggcggagtt
    10981 gatcatggcg tagcggccgg catcgttgtc gggcaggtac gcccagtcgt cggtgcggaa
    11041 cgccgtcatg ccgatgttgt ccggcgtctc gagcagtttc gtccccatga tgccggtgcg
    11101 gaccaggggc ccccagccgg ggtcgacgcc gacgacatcg taaatccagg ccagattctc
    11161 ggactggtcg aaggccacga catcctggag gcggccgttc tcgccggtgc cgtcgggacc
    11221 gccgacgtcg atgtcaatgt agaggccgaa cgcgaagtcg tggtaatcgg tcgtcgaggc
    11281 gttcgtgatg tcgtaggaga cgaacaggaa gtcctcgttg tagcagtagt tccactggta
    11341 gatcgtctgc gtcacctcca gccccatgag ggatgagccg ccggcgttgt cggtgaaccg
    11401 caggtgggaa tcctgcatgg aggtcgggcc gccgggccgg aagctggcgg agaggctgtt
    11461 gtagacctcg ttgtagtccc actggtcggc ctccgaatcc cagacccgcc agccgcgggc
    11521 gggattcccc tcgcccgccc cgaccgtgtc ggtcggatca tagagatagt agcgggcggt
    11581 gtccgtggac aggaggatct tgtaggggtt gtcggcgaaa tcagggttgg ggagggcctg
    11641 gaaatcatcc tcggtattgg ccaccagggt atcgccggtc ggggtgatgc cgcccatcca
    11701 gagcatcaat tcgccgaggt aattgcggcc ggagttccgg ggccactcac ccgagggcag
    11761 gccgtagtac cagtagccgc cgatgtagcc ccagttgtcg acggtggtgg cgatgtttcc
    11821 gacgttgtgt gtgatctgat cccaggtcgc cgggtggggc gacagttgct ggatctgtac
    11881 cggcgcgccc ggacgcggct gcgggggcgc catcgctccc gccgcagccg ccagcaggca
    11941 cagcgccgaa accgagatca gacaacccgc cgtgacggta tggtagaagg tcttcatcgt
    12001 tatctgctcc ggtttagaaa ttcagctgca gccccgttct gatcgtccgg ggcggcgcgt
    12061 agttttgcgg gtcgttgtca tagagccggt catagtggtc gagcgcctgt tggctgaccg
    12121 acagactgcc catgtaatcg accccgtcgt tgtccgggag gccggtgctg gtgtagaggt
    12181 tcaccacgtt gcgccggtca aacaggttgt ccacttcgac aaacaggctc agccgcgatc
    12241 cggcgcggag cgggaagtcc ttgttgaagc ggaggtcgac cttgtagttg gcggggaggc
    12301 gccaggcgtt gcgctcgccc acccggttgc cgctctggtc ggtcttggtg tacggcaggc
    12361 cggagccata gtacccgacc agggtcagcc cccagtcgcc cggcagcttc tgacccagca
    12421 gccggccgtc ccagtcggac ggcacccggt agctcaggac gccggtgacc gtgtggcgct
    12481 ggtcgaagtc gagggggtac tcggtgaacg gctgcacggt gtcggtggtc gaggtgatgt
    12541 acgtgtagta gggttcgagc gcgtacgagc cattgccggt cgcgatcatg tacccgtagg
    12601 agaccgaccc ggtgaagcag ccgccgttgg gcagcacttc cagggcgagg tcgatcccct
    12661 tgaccgagcc gtagtcgccg ttggtgatgc gggtcacttc ccgcccggcg atcaggccgt
    12721 cctgccgcgc cgtcaccaga tcctcgatgt ccttgtagta ggcggtgaca ttcaggtgcc
    12781 agcggttgtc gaggaggtgg tcgaggccga gctcatacga ggtggtccgc tcgggttcga
    12841 ggtcgggatt ccccagcagc ggcagaccgg tggtgatgtc gccctggtag ttcatgtaga
    12901 ggtactggaa gcggggctcc tggtagtaca cgccgtagtt gaagtgcatc ttgctgttct
    12961 ccgcgatggg gaacgagacc ccgaggcggg gggacagccc gtgcttggtc tcggccttct
    13021 tccacaccgg cgtcgccgcg tacggatcgg cgttgtagct gatgtcggcg ttgtggaaat
    13081 cgtagcgcag gccgaggttg acgatgaagt agtcgtactc catcttatcc tgcacgaaga
    13141 acgacgcgta ggtcggcttg ctctggtact tctcgccata gggccggtcg ttgaagaact
    13201 gcttgaagtc ccagttgatg tcgtactgcc ggaactcggc gcccgtcttc aactggttcc
    13261 acttgttcac ctggtgcatg aaacgcagcg agaaggcgtc gtaccacgtc tcgcgcagcc
    13321 ggtaggtcgg gtagtagtcg tcgccgacgg tgaaaccgac cacctgcatg ggatcggagt
    13381 agtcgggttc gttcaggtag ttctcgtcgt cgatggtgcc gttgtacacg ccggcggagt
    13441 cgaccgcgta gcccggccat tcgtcccagt aggtatcgaa gaggtgatcc ggggccgatt
    13501 tcgtccgggt gtggaaacgg ttgaccgcgc cggtgaggac cgcccgctcg ttgaacgcgt
    13561 agtcggtgct gaaccccacc aggtaggcct ccttctcgaa cacggggaga ccatcgagat
    13621 tgaaatcata cgaccggttg ttgacgtcgc ggtggtcgta caccgaaccg tcggccgagt
    13681 agtatgtgac gttggttttg aacttccacc cgctggcggg atacatggtg agcttcccgg
    13741 tcccggtcca cgaggtgacc tcgttgtgcg gcagggaggt cgggtcggtc aggtactggc
    13801 cggcgctgaa gaaggtatag cgccgggggt cgagccccgg gagcggcccg gagaggtcga
    13861 aggccaggga gcggttgccg atccgttcga ggccgcccca gtcccccttg tccacgtcgt
    13921 aggggtgggt gaatccttcg tacatcttaa ggcggccgtg gtactgcgcg gtgccgtcgc
    13981 gggagaccgc gctcacgacg ccggagaggg cctcgccgta ctcggccgtg tagccgcccg
    14041 aggtcagcga cagctcttcc agggccgagg ggatgatctc gattccctgg gtcgcggtga
    14101 acggatcctg aatgttgaat ccgtcataga agtacgtcac ctggccggcg cggccgccgc
    14161 ggacgtggag cgcctgctcc cggtcttcga ccacgcccgg gtaattccgc agcaccgact
    14221 ggacgctcac gatgttgggg agttcctcca gccggtcggc cgtgaagatg atgcgggtgg
    14281 cggtcaggtc ctgctggacg atggggttgg cggccgagac caccacctgc cggccgagtt
    14341 cgacgggcgc gggcaccatg cggaagtcca gcggggtggt caggtcgatg agcacccgca
    14401 cgcccgtttt ggtcaccggt tcgtacccca tgtaggacac ggagacgtcg tgggagccgc
    14461 ccgggaggtt gataatgaag tactcgccgt ccacgtcggt cagggcgccc atgtcggtgc
    14521 cgacgacgcg gatgatggcg ccgacgatcg gctcgcccgt cgcgctgtcg agcaccaccc
    14581 ccgacacctt gccggtgacc ccggcctcgg cggcggaggg cagtgcgaga acggcgaggc
    14641 agccgaccac gcagaagatc atgtgcagcc gattacgcaa ggcaaatcct cctcgcatat
    14701 gtttccacaa tcgaaagttg tttgcttcct cgatttatgt cgatggtccg cgccgggcgc
    14761 ggaacgcagc ttgccggtct tttcgagagt atcggcggcc cctgtaaatc cttgacaccc
    14821 ggtcggggat catcgagtcc tttttttgcc ggcgctttcc gccgtccgga tcaagtttcc
    14881 ggggggccgc gccgatatct gagacgtgtc atgacggcgt cacgcccgga ttcgacggga
    14941 agcaacggtg acggctacaa cgtcgaggaa acagagagga taagcgtgct cacccgatga
    15001 acagcgtgat gaaatgggcg ttcgcggcag ccctcgccat gagctgcctc gcctcaggag
    15061 caacgccggg gagatccgaa actgtcgccg tgctgatgag cgacacgctc acgtcgacca
    15121 cgaggactct gcagggggcc aagaagacga ttgaagcggc caatccgggg attgagctgc
    15181 gcacgttcat gatcggccag tcgccggccg gcgaccaggc ggtcgccgat tcggtccgga
    15241 gctgcaatcc ccggctggtg ctgaccatcg ggagcacggc cacagggatc gcccagcacc
    15301 acctgccgca ggtcccgatt gtctttgccg gcgtcatgta cccggtcctc tccgggtttg
    15361 tgcagacgat tcaggaaccc ggcgggcaca tcaccggggc gtcgctggat attcccaacg
    15421 aagttcagtt ccgctacttc aagaagatca tcccggggat cgaacgcatc ggcgtcctct
    15481 acacggcgaa cacggccagc ctgattgccc cggcccgggc ggtggcccgg ggcatgggtc
    15541 tggaattgca cgcgatcgag atccgcgaca gccgcgagat cccgcaggcg ctcgattcgc
    15601 tggcgttgac ggtgcagggg gtgtggtcgg tcgccgaccc ggtgttgttc gacccgcagg
    15661 cgacccggta cattctgctc aacacgctgc gcaagaatct gccgttcatg ggtttttccc
    15721 gctacgtcgt ggagtcgggc gctctcttcg cgctcgattt cgactacaag gcggtgggga
    15781 tccaggccgg gcagatcgcc aaccgcgtgc tcgccggcga ggctcccgcc tccgtcagcg
    15841 tctcggccgc cgacgtcatc tggttccact acaacgagaa gaccgcccag cacattcacg
    15901 tgaccatccc cgacgaactg gtagccgtgg ccaaggaggt ttaccgatga gcttccttga
    15961 gcgcatcatg agcttccgcc tgcgcaccaa gttcgtcgtg ccgatttcgc tcttgctgat
    16021 cgccagcatt ctcaccgtga gcggctacct catcaagcgg caggcggacg gcttccggcg
    16081 cgaactccgc acgagcggcg agacgatggt ccggatcctg gccatgaacg ccgagagcgg
    16141 cgtcctcttc gagtcgcgct acgagctcca ggagctgctc cagattctca accagttcga
    16201 cgccgtcacc tacgccgcca tctacgcgga ggccggcaag ccgctcgcct cctacggcat
    16261 gtggagcgag agcgacatcg tggtcaagcg cgcgctcgac cgcggcgccc gcgccgacac
    16321 catcggcggc ggtttctacg tgaagaatgc cgacggccac gagtttctcg aactgaacta
    16381 ccccgtgtac tcccggaaag aaatcctctc ccgggagacg ctcggcatga cgagcgggat
    16441 cgaccagtcg ctcggccagc gctacgtgac cgaacagatc gggtcgatcc ggctgatcct
    16501 ctcgctggcc aaggtcaacc tgtcgatcgc cgaggcccag aaaaccgcca ttctgctcac
    16561 cgtggtgatc ctcatcctca ccatcctcgt cctgactttc ttcgtgcgcg tcgtcaccaa
    16621 gcctgtgcag atgctcgtcg ccgtcaccga ccaggtgagc cggggcgatc tcagccgcct
    16681 ggtggagatc aaccagttgg acgagatcgg ccacctcgcc aacacgttca acaagatgat
    16741 cgcgagcctg cgccagagcc gcgaggagat cgaggagtac aaccgcaatc tcgaggagaa
    16801 gatcatcgag cggacgatcg ccctcgagga ggcccaggcc cagctcatcc agtcggagaa
    16861 aatgagcgcc atcggccagc tcgcggccgg cgtggcccac gaattgaaca acccgctggg
    16921 gggcatcctc gggtacgccc agttcacgct cgagaagatg aagaagaatg ccgcggccgc
    16981 gggcctgacc aaggaagccg agagctacat ccgctatctc accgatatcg agacccaggc
    17041 ccgccggtgc aagaacatcg tccagaacct cctccgtttc tcccggtcct cccacaccac
    17101 ggagttcgag gacgtggacg tgaaccaggt gatcgaagat acgcgcacct ttgtcgagca
    17161 ccagctcctc atgaatcaga tctcgctgac ggtcgacctg gccgaagatc tgccgttgat
    17221 ccaggccaac gccagccagt tgcagcaggt cttcacgaac ctgatcatca acgccatgca
    17281 cgcctcccgg tccgattcgc agatccggat cgagtcccgt tacagcccgg ccgtcgggga
    17341 gttcggcggc gccgtcgagc tccgcttcat cgaccagggc cacggcatcc cccccgaaat
    17401 catcaacaag atcttcgagc cgttcttcac caccaaggaa gtgggcaagg ggaccggcct
    17461 cggcctctcc gtgagctacg ggatcgtcaa ggagcatggg ggggaaatca aggtcgactc
    17521 ggcggtcgga aaaggctcca ctttcactct gatattcccc gttcagaaac acccggtcga
    17581 cgccgataat tctctaaaga gcgggatagg agtgtaagta cttccgaact tgcgggatgt
    17641 ttcaatggat gaatcggtca gacgttccgt cctcgtcgtg gacgacgagg aaatcattcg
    17701 cagttttctg tttgaagtgc tcagcgagga gtacgacgtc agcctggcct gcgacggcga
    17761 cgaggcaatc gagcagatcc ggaagaggcg ctacgacgtc atcattacgg atctcaagat
    17821 gccccgggtg tcgggcgagg aggtcgtcaa gttcgcctgc gacagggatc cgcagtcgaa
    17881 agtcattgtg atttcgggct tttcaagcct atacacggtc agtcaatcca taaacaacgg
    17941 cgcctgcgca ttcctctcga agcctttttc catcaaggaa ctgatgcgga cggtcacgaa
    18001 tgccctgtgc gcctagggac acggcaatgg cacgaatact gatcatcgac gattccgagg
    18061 ttattcgcaa cctgctggaa gagtatttga ccgacgccgg gtacgaggtc gagacggcct
    18121 gcgacggccg ggaggggatc gaccgggctc tgggggggga tttcgacgtg gctttctgcg
    18181 acatccacat gccgcacaag aacgggtacc aggtgttccg ggaggtgagg cggcgcaagc
    18241 ccgggatggc gttcatcatg accgactctc tgcccgatca actggccgaa atggcccaga
    18301 acgaaggcgc ccactgctgc ctgaccaaac cgtttgatct gaaccaggtc cgccgcgtcc
    18361 tggagacgct gctggccaaa gcgcgcacgg tatgagcggc acggaaactc gcagtataaa
    18421 tgtcctcgtg gtcgacgacg agaccatcgt cctgtccctc gtgcgcgatg cgctcgagga
    18481 cgaggggtat gagattacga ccgcggtcgg gggggacgag gccctgcagg cgctcgactc
    18541 ccggcccgtc gaccttctca tcaccgatat ccgcatgccg cacatggacg gtaccgagct
    18601 ggtgcgccgc gcccgcgagc gcaaccccca catcggcgtc atctacatga cgggctacgc
    18661 caatctgaat tcggccaaag acgcgatcaa acagggcgcc ttcgactata tcctgaaacc
    18721 gttcgagctg accgaaatcc gccaggcggt gcacaaggcc gcggggaaac tccgcgagcg
    18781 ggccgggcag gactcgggcg aacagctcga acgcctctcc gacctcaacc agatgctcgt
    18841 cacggtcggc gaccgcaagt cgctggcgag cgtctccctt cgtttcgcca tgatgcattt
    18901 cgacgccacc cagggggccc tcctccactg ggaccgggag cggaccgcgg tggagacgat
    18961 cacgattcac ggcgaccagc ttcaggagac ggcgctggac agcccggcgg tgcgggagtt
    19021 catccgcgcc atcgacccgg cctcgttccg ggagccggtc attgtcacgt cgccggagcg
    19081 gcacccgctg gccggcccgg cggcggcccc ggagatccgc gcggccctgt tcccctcctg
    19141 gctcaccgaa gggagcgcca tggtcgtcat gccgatcaac cgcgcgaaca ttctgtatgg
    19201 catcatcatg atcaggtgct cccccgacgc cactgcgctc accgggaccg acacccggct
    19261 tttgaacatc gccgccggcc aactggccgt ttcgctggag aatctgtttc tgctcgagga
    19321 gacgcagacc gcctacgcgc ggctcaagga gatgcaggac gagaccatcc agctggagaa
    19381 actggccacc cgcggggaga tgtcggcgga gatcggccat gagctgaaca acttcctcgg
    19441 ggtcgtcgcc ggcaacattt cgctgctgga ccaccagctg aagaagtcgg gcgcggccac
    19501 ggccgaaaag tacatgagcg ccatccagac caacatcgag aagatgaagc agttcacgag
    19561 caacctcatg gacctcaccc cgatcgcctc gaaaaaggaa acggtctact tcgaccaggt
    19621 cctggccgaa gtcatcgact acctcacgcc gcagaagcgt ttccggggag tgtccatcga
    19681 ccacaccgtc gaccacgagt caattccgtt cgaggccgac agcatccaca tccagcagtt
    19741 gctctacaac ctgttcaaca atgcggccga cgccacccgc gggtgcgcgc gccgggagat
    19801 tgtgtcgcgc gtcgagtacc ggcccggcga accgcacttc cgcttcgtca tccgcgacac
    19861 gggcgtcggc atcgaaccgg aacttctcgc caaggcgttc gagcagaagt tcacgaccaa
    19921 gcccgacggg cacggattcg gcctgctcgt ctgcaagcgg atcatcgacg cccacagcgg
    19981 ccgcctccac gtcgactcca cacccggcga ggggaccgcg atcagcattg aattcccggt
    20041 ggccggtgcc gcggacagcg tcgacgcgac ggccgggcag ttcgccatca ccgacaagat
    20101 ccccgtcggc tgacccgcct ctccctcaca acgccgcgga acgaaagcgg ccgcgccccc
    20161 ggggaccgca tgccagccgg aggaggccag ccacggaggc ccggcgcccc tgcgccgccg
    20221 catctcggga gctctcaggg gacaatccgg taggggacgc accagccggt ccaggtcagt
    20281 tgcgagtcgg gcggctgggt tcccggaatg gggacgtatt cgaaatcctg catgaacagg
    20341 aactcccaca aacgcccggc cgtccccggc atctggaggg tgtccacgaa gcgcccggga
    20401 ccggccggct gcatcacgcg cacttgctcg cccgcggccg tcgcggccga gagaagccgg
    20461 tagcgcgtcc tcagcgacac atcccggcag tcggcgacaa ggcgctcgcc cggcccgacg
    20521 gcgcccaagc gatcgagccg gcggaacacc tggcggctcg tctgcgccgc ggtgaccgtg
    20581 gagcagggaa gcgccggatt gctcgtcgag agacactcgc gcacgtacag atacgggatc
    20641 tggtcgtagc ccgccaggtc gccggcgaac tccgcccccg actccgtccg caccgccata
    20701 ctccccgtcg gcgccgcgcc cccgttgtag gcccacagtt tccatccgac atacggctgg
    20761 tcatcgccgc ccagtttcag gaagtagccg tagcgcgtga tcgcccaaac gtgcgacgcc
    20821 gtggccgtct cctcgtcctc ggtgcgcgac agccggacgt agaagtagtc gtcgacggtg
    20881 gcctccgagg cccagaggcg cccgaaggga ttgccgtagt catggtagct gcggtagtac
    20941 tccaccgagc cgtccggcca ggtgcgtttc ttcacggtgt cgatttcggt gtcaagcgtg
    21001 atgatccgcc gggttgaatc gacgacgatg cggtaccggg cgggatcgtt cggaatccgg
    21061 tattcctcgg tggggaacag accggtggtg cggaagaggt cgacggcatc ctccgaggtg
    21121 gcgaggtacc ggagcagtat gtcgcggtcg acgacgacgg gaacgtcctt ctctccacag
    21181 cccgcgagcg cggcggccag catgacggcg ccggcggcgg cgcggatgaa cagaggcagt
    21241 ggtcgcggca tcggctattt tccctttttc ggtttcgggt ttttccgccc gtctccgcgc
    21301 agcgcattga tttcgtcgcg gatcatgatg gccgtctcga agtcgaggtt ctcggccgcc
    21361 ttgcgcatgg ccgcgagcat gaacgcgagc tggtcctcgc gggacatgcg ggtgaaactg
    21421 ctcggtttct cgaagcgcgg cgcctcctcg ggcgtcttgc tgtcggcgaa ctgggtggag
    21481 cggaggatct cctcgcgcgt cttgaaaatc gtttccgggt cgatgccgtg ctcgcggttg
    21541 taggcggcct gcttctcgcg gcggcggcgg gtctcctcga tcgccttgcg catcgattcg
    21601 gtgactttgt cggcgtacag gatcacctcc ccgcccttgt tgcgggcggc ccgtccggcc
    21661 gtctggatga gcgaccgctc cgagcggagg aatccctcct tgtcggcgtc gagaatggcc
    21721 accagtgaca cctcggggag gtcgagtccc tcccgcaaga ggttcactcc gacaaggacg
    21781 tcgaactccg ccaggcggag gtcgcggata atgcccgtgc ggtcgatcgc gtcgatctcg
    21841 ctgtgcaggt agcgcacccg caggccgagc ttctccatgt agccggtgag gtcctcggcc
    21901 atgcgtttgg tgagcgtcgt caccagcacg cgctcgccgc gctccgcccg ccgcctggcc
    21961 tcggccagca ggtcgtcgac ctgcgtcccg agcgggcgga cggtgatgac ggggtcgagc
    22021 agaccggtcg gccggatgac ctgctcgacc acgatcccct ccgccttggt cagttcgtag
    22081 tcggccgggg tcgcggacac gtacacccgc ttcagcacca cccgctcgaa ctcctcgaat
    22141 acgagcggcc ggttatccag cgccgagggc aggcggaaac cgtgctcgac gagaacctcc
    22201 ttgcggctgc ggtcgccggc gaacatcccc cgcacctggg ggatggtctg gtgcgactcg
    22261 tcgataatgg tcaggaagtc gtcggggaag aaatccagca gggtgaaggg gcgctcgccc
    22321 ggccggcgcc ccgacaggtg ccgggagtag ttctcgaccc cggagcagta cccgacctct
    22381 tcgagcatct cgagatcgta cttggtgcgc atctccagcc gctgggcctc caagagcagg
    22441 ttgcgggagc ggaagaactc cagccgctcg gccagttctt cgcggatgcc gcggatcgct
    22501 tcctgcatcc gctcgggcga ggtgatgaag tgtttggccg ggtagatggc gatccggtcg
    22561 cgctcgcgca gcacctcccc cgtcagcgga tcgatctccg tgatccgctc gacttcgtcg
    22621 ccgaaaaact cgatccgcat ggcgtggtcg tggtaggccg ggatgagttc gacggtgtcg
    22681 ccgcggacgc ggaaatttcc ccgcgagaag tcgatgtcgt tccggttgta gtggatgtcg
    22741 atgagcttgc ggatgacctg gtcgcggttg tactcccccc cgcgcacgag cgggatgtac
    22801 tggttcttgt attcgaccgg cgacccgagg ccgtaaatgc aggagaccga agccacgatg
    22861 atgacgtcct ggcgctcgag cagcgaggcg gtcgcgcgca gccgcagccg gtcgatatcc
    22921 tcgttgatcg aggtgtcctt ctcaatgtac gtgtccgtgg tcggcaggta cgcctccggc
    22981 tggtagtagt cgtagtagct gatgaagaac tcgacggcgt tgtcggggaa gaaagccgtg
    23041 agctcgccgt agagctgggc ggcgagcgtc ttgttatgcg acagcacgag cgcgggacgc
    23101 ccgtactggg cgatcacgtt ggcgatggtg aacgtctttc cggaaccggt gacgccgagc
    23161 agcacctggt gcatgcggcc ttcccggagc cccgcgagca gttgggcgat cgcctggggc
    23221 tggtcgccgc ggggcgagaa ctggctgcgg agccggaacg gggtgtggcc ggcggcggtc
    23281 ggggcggttg gtttggccat gtggttcatc gtcctccact gacgcaggtg cgccgcaata
    23341 tatgcaatac aaccgagggg gcaatggcga ttgttcgcgg ccggccctgg tgccggtcgt
    23401 ccacaattgg aacccgttgg gcgaccgcgg gtttaaaagg tgatttggac gtcttcgacg
    23461 caagtttggc cttgacttgg cgtgcaaaag gtatacttta tgcggtttaa cctgatttgg
    23521 gataacagct ttccacgttt ggaggaatgt tcatgtacgc tgtcttcgaa gtcgccggtt
    23581 ttcagtatag cgctcaggaa ggggatgtcc tccgggtacc ccaccagaaa gccgaccccg
    23641 gcacgtcgct ggccatcgac cgcatcctct tagtccgcgg cgacggggcc acggcgatcg
    23701 gtacgccgac ggtcgccgat gcgcgcatcg atgcggaagt tgtgggtgaa ggcctggccg
    23761 acaaggtgct catctacaaa tataagcgcc ggaccaagta ccgccgcacc caggggcatc
    23821 gccagcgttt caccgagatt cgcatcaaca aaatccacgc ccccggcgcc tgagcgcagt
    23881 tgagtcggac gaacggaccc cgagcaggga ttgctcgcgc ctgtctcttg gcggcgctgg
    23941 tgctgagcct gtcggctcac gcccagaata ccgcggagca gtttcgggtg gtcgatatcc
    24001 aggttgaggg caaccgggtg gcgacccggt cgctcatcct gggcgtcgcc tctcttgagg
    24061 ttggcgcggt tctttcggtc accgacgtct ccgagtcgat ccgccgcctc tacgccctcg
    24121 gcatcttccg cgatgtccgc atcgaggccg aggaggtgac cggcggcatc cggattttca
    24181 tcattgtcga agaactcccc aagctggccg ggttggcatt cgaggggaac aagaagctca
    24241 aggacgacaa actccgcaag gagatcggcc tgggcgtggg cggctacatt tccccctacc
    24301 tgatcgagaa gagccgggcg aagattttcg acctgttcgc cgccgaggga tatttccggg
    24361 ccaagatcga gccggagttg atctacggcg ccgattccgt cgaggcggtc ctgcagttca
    24421 acattcgcga gcgcgacaag gtcaaagtcg agcaggtcgt ggtcacgggc agccggcggg
    24481 tggaggccgg cgacctggtc ggcaggatgc gcaaccgcaa gcgcggtctc ctgcgcacct
    24541 ccgacttcgc ccaggacaag tacgacgagg acctgcagaa agtcatcgac gaataccaca
    24601 agcgcggttt catcgacgcc tacctcatca acgattcgct ggcgatcgac acggcccgca
    24661 accggatgac catctacctc gacgtctacg aaggcccccg ttactatttc ggcgagacgt
    24721 cgttcaaggg aaacgagagg ctctcggcgg accatctcgc gaagctgctg aagcacaaag
    24781 cggggcaggt gttcgactcc gagaagtacg acgcctcgct cggggaggtg gcctccggct
    24841 actacgatat cggccacctc cacgtggaga ttttcgacga gcggtcgacc cggtccgact
    24901 cgctcatcga tatcacctac gacatcaccg aggggctccc ctccaacatc aacctcgtga
    24961 agatcgtcgg gaactacaaa accaaggacc gggtgatccg gcgggagctg tcgacgctgc
    25021 cgggccaggt gtttgaccgg acggcgctga tccggtcgat ccgcgacgtc atggcgctga
    25081 acttttttga caaagtcgag ccgcgcccgg tgaacctccc cagcggcgac gtcgacctgg
    25141 tgtacgaagt ggccgaaaag cagaccgggc aggtgtcggc cggcgccggg tacaacagcc
    25201 aggacaaggt ggtcggcacg ttcggcatgg gcatcccgaa tttccggggg ctcggccaga
    25261 acctgtcttt caacgtcagt ctcggcaaat cgtacaactc cttctcggtc tcgttcaccg
    25321 agccgtggat gttcggctat ccgacctcgt tcggagccag cgccttctct ctcaaccgcc
    25381 gctggtacgg cgactacacc gaggggcggg cgggcggctc gctccgcctg gggcgacggc
    25441 tgcgctggcc ggacaactat ttccgcgtct acgcctccta tgccctggag cggacgcgct
    25501 actacgattt cgacgcgacc tttgcagcct ccaattccta ccaatcgcgg tactactaca
    25561 acaacccgga gaccatctcg ccggcggatt ccctcatggc cgcccggacc tatgggccgt
    25621 acccgggttc cattctcgag tacgaagact ggcagacggc atcgcgggtg tcgctgacgc
    25681 ttctgcgcga ctcgcgcaac cttccggaat tcgccaccaa gggttccatc ctctcgtaca
    25741 cgctcgagtt gaccgggggg ctcctcggcg gctactggga gtacacgaag cacgacttcg
    25801 aggcggccaa gttcatcccg ctcatgtggg gctgggcgct cgccgccaag gcgcagtacg
    25861 gggttgtgac ggccccggct ggcgacgccc gcatcatgat ctccgaccgc ttcacgcccg
    25921 gcggcaccgc ctacgacggc atcgtccgcg gctacgacga cggcagcctg acgcccgact
    25981 cggccatcac caccgacacg atcttctact acagcaatga aaacgcggtc atcggggtcg
    26041 atccgcccga cggcaggtat tttccgctgg acaccacccg ggtccgcggc aagtacatgt
    26101 tcgtggccaa cgtcgaagtg cagataccca tcgccgcccg gcagctctac ggcctgctgt
    26161 ttttcgacgc cggcaactcc tggctctacc gccgcgacat cgatctgctc aatgacctgt
    26221 ataagagcgt gggattcggc ttccgcatca tcgtcccggg catcggcacg ttagggtttg
    26281 acttcgggta cccgcttgac gatgttgaag gcgaaccgag aagctggaag ccccatttcc
    26341 agatcggaac aacattcaga taagaatacg agcaagtgag gattcgcatg cgagtaacaa
    26401 aagctgcatc agttctgtcc gccgtcctgg cgctcacagt catcctgtcc ggggcggccg
    26461 ccgcccagac cctgaagatc ggtttcgtgc gcgacgaccg catctacgcc gaatacaccg
    26521 agtggcagcg cgcccaggaa acctgggaga ccgaccggaa agcgtgggac gacgaagcca
    26581 gcgccaaaga gcaggaactt caggagctca ccgccgacta cgagaaacag cgcctgatcc
    26641 tctccgacga gaagaagcgg gagcgcgagg cggctattcg ggccaagcgc gagggtctcg
    26701 acgcctacac caagcagatc tatggtccgg gcggcaccgc ggaacagcgg gaggcccagc
    26761 tgatcgagcc gctgttgacc aagatccgcc aggccatgga ggcggtggcc accgagaaca
    26821 gctacgacgt catattcacc ctccagggga tcggctacat caaggaggag tacgacgtca
    26881 cggacaaggt gctggcggcc ctgaacgaaa tggagtaaag ctccgcgccg ccgccgatcc
    26941 ccggtcggag gcgggcggcc gcggggcgcg aggactcatg gccttcaccg ttgcatatct
    27001 ggccgagcgc gtcggaggga ccgttgccgg cgacggcagc gtggagatcg gggcagccgc
    27061 gccgatcgag acggccggtc ccggcgacat cagcttcatc gccaacccgc gctacgcgcg
    27121 ctttctcgcc acgacccgcg ccggcgccgt cgtcgtgagc ccgcagaccg agtgcccccg
    27181 gctccccctc atccgccacg cgcagcccta ccttgccttt gcctgcatac tcgacctgct
    27241 ctaccccgat gaacgcctgg tcgctcccgg catcgacccg cgcgcggtgg tggccgcggg
    27301 cgcggtcgtg gacccgacgg ccggggtcgg cgcgctctgc cacattcgcg agggggtgcg
    27361 catcggcgcc gggaccgaac tcgtgtcctc ggtttttgtc gggcgcgacg tcgtggtcgg
    27421 ggacaactgc cgcatctacc cgggggtttg cctcatggac ggcacgcgcc tcggcaacaa
    27481 cgtcatcatc cattcttcga cggtgatcgg gtccgacggt ttcgggtttg cgccgtcacc
    27541 gaccggattg aagaagatca aacaggtggg gtgggtggag atcgatgacg atgtggagat
    27601 cggatcgaac tgctcggttg accgcggggc gctcggaccc acccggatcg gccgggggac
    27661 caagatcgac aacctcgtgc agatcgccca caacgtcgag atcggtcggc acgcgatcat
    27721 cgtgtcccag gtgggcatct cgggctccac acgggtcggc aacggcgtgg tgctggccgg
    27781 ccaggtcggc ctggtggggc acattgaagt cggcgacggc gttcaggtgg gggcccagtc
    27841 cggggtcgcc cgctcggttc ccgcgggcaa gaaagtcttc ggttcgccgg cccgcgatct
    27901 ggcggagacg ggccgcatcg aagcggccct cgtgcggctg ccggaactgc tcaaacgggt
    27961 caggcggctc gaagacaagc tcggcggcga gtaggtctca cgtcttcagg cgctcctcaa
    28021 tctccgcgat cttctcccag ttcgcctgca tctgctcaat aatgcggacc agttgccggt
    28081 gtccatccac aacggtaatc tcccgcatcg cctcgaggag ttcccactgc tggcggttga
    28141 tcactttcca ctgggcgatt tggtggctca gattcgaggt ggcgaggttc agttcataca
    28201 ccagttcctg cacgtgtggc gccggcttgc gcccctggat tctggaggcc aggtcgccgt
    28261 tggccagcgc ctgcaggcgg tcgcgtatga cggtggccgg ctggcgcaac tgggccgccg
    28321 gcaccatcag caggacgcac agtgccgtga tcgccgcgac cgccgccgcg atcatgtacg
    28381 ggaccagcgt gagagccgcc aactccacgc cgctgccgat ctgctcaagc gtagagcggg
    28441 agagggtcca gttgaaggcg atgatgatca gcgccgagag ggtgacggcg gacccaatga
    28501 ggagcatcgc ccgggtggcc acctgctggc cctccggggt atcggtacgg gggatgaagc
    28561 gcgaggcata cgacatagtc tgtccctcct atgatcgttc cgaggtaacc ttgtagtcgg
    28621 ctcatcgacg gtggggcagg ctgggcttag cccaatctcg gcgcattctc cggaactcgc
    28681 ctgaacagta aaaggccctg tcatccaatg gattacaggg ccccgtgaaa ccgttggtca
    28741 gggtttaatc agaacggaag gtcgtcctcg gcgggtgcgg ctccctcggg ggccgcggcg
    28801 tcttccgcgg ccggggcacg ctcgccgccc ccctggccgc caccctcccg gccgctgccg
    28861 ataaactgga agttctgtac gacgatttcc gagatgtgcc gcttgttgcc gtccttgtca
    28921 tcgtaggacc ggttgacaat ccgcccttcg atatagacct cgcgcccctt ggcgaggtac
    28981 tcgttcataa tctcggcggg gcgtccccag gccacgatgt tgtgccaggt ggtctgctcc
    29041 tgtttctggc cctcctggtt ggtccaccgc tcggtggtgg ccagggtgaa agtcgtgacc
    29101 ggccgaccgc tggccgtgta cttgagctcc gggtctttgc ccaggcggcc gatgaggatt
    29161 gctttgttga ctcccataat catttacctc tagaggttag gcgcactcgt tctccacctg
    29221 ctgcggcggc cgctccggaa ccacggcctg tgcacgagct ggggcgcgcc gcggtcgaga
    29281 ggtccgcggg ctcacgccgg agcatctccg cacggaacga acgggacctg ccgtgcgttc
    29341 atgggtacaa tatttagctt gtctgcacaa gcgcaatcac atatttagtg gcgatttcga
    29401 aagaatctat tgtgtgctca gccatggaag ctcgcccgga aatcgacctc gaccgcgccg
    29461 tgaccacgat cacgcggttt ctcaccggca agctgctgca gtcgggcctt gacggctatg
    29521 tcgtcggcgt atccgggggg ctggactccg ccgtggcggc tgctctggcg gttgcggcgg
    29581 tcggtcccga caaagtactc ggtctgcttt tgccctaccg aacttcatcg gcggagtcgc
    29641 tgcacgacgc ccgtctgatg gtggaacggc tcggcattcc gcaccgggtc atcgacatct
    29701 cgccgatgat cgacgcttac tacccacgga tcaccgatga gctcaagcgc cgtgccggca
    29761 acaagatggc gcgggagcgc atggctatcc tgttcgacgt cgcgctggag acctcgcggc
    29821 tggttctcgg gaccggcaac cgcagcgaaa ttgcgctggg ctacacgacc tggcacggcg
    29881 attccgcctg cagcgtcaat ccgatcggcg agctgtacaa gaccgaggtg cgcctcctcg
    29941 cccgcaagct ggcggtcccg gaaccgatca tcgccaagcc gcccaccgcg gatttgtgga
    30001 aagatcagac cgatgagggg gagatcggcg tcacctaccc gcagatcgac cgcctgctgg
    30061 cccggattgt cgaccagggc gttcgttcca tgagtcaact ggcggcggag ggtttcccgc
    30121 ccaccgaaat atcgcgggtc gtgtcgctgc tcaaccgcaa cgccttcaag cgcgccttgc
    30181 cggaggtcgc ccccctgcag cgaaagccga tcccggcgaa cgtgcaactc tcggaataag
    30241 ggggtcgtta tgcccgagtc gcccggcctg ctctatctgg ttccgacgcc gatcggcaac
    30301 atggccgatc tgacccgtcg ggcggaagaa atgctggccg ccgccgacct ggtcgcctgc
    30361 gaggacaccc ggcacagcgg ccggctcctc aaggcccttg gtctgaataa gcggttgatc
    30421 agctaccatg atttcaatga gcggacgcgc gccgagcagc tcatcgacct catccggcag
    30481 ggacagacgg tggcggtcat caccgatgcc ggctcgcccg gcatttcgga tccggcctac
    30541 cgcatcgtgc gcaccgccat cgacaacggc attacgatca tccccttgcc cggtccgacc
    30601 tcgatcatcc cggccctgac ggcatcgggg ctgccgaccg accggttctt tttcgagggg
    30661 tttctgccgc acaagtcggg agcgcgccgc acgcggctgg cccggctgac cgagtttgac
    30721 cacaccctcg tcttcttcga atcgccccat cgcgtggtgc ggtcgctgga ggacatccgg
    30781 gaggtgctcg gcgatcgtcc ggcctgcctt gcccgggagc tgtccaagct gcacgaggag
    30841 ttcctgcgcg gttcggtctc ggagatcctg gccgcactcg gcgggcgcac ggtccgcggc
    30901 gagatcgttc tcatcgtcgg cggcgccgat tcgcttcggc agcctcggga ggagggctga
    30961 gcgcgtgggt ccggctcttt ctcgcttcta ccttttcgat cggatcctct tcggatactg
    31021 cctcttcatg gtcgggctgc tgctggcagt cgggcggcct ctcggc
//
LOCUS       DAOPOE010000030        30363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1957_length_30363_cov_6.157054, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000030 DAOPOE010000000
VERSION     DAOPOE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30363
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_30363_cov_6.157054"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PKM94_05745"
     CDS             <1..1432
                     /locus_tag="PKM94_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOD66179.1"
                     /translation="DLTEIIAAERGLRLDREGFDRAMERQRTQSKAGARFAAQVSSLN
                     EVLDAAGLRAAPTVFARDRFEAAASIQHVFPWPGGDPRRVGIILDTTPFYTEAGGQVD
                     DTGVIVGPGAAVRVEAVHGYRDWSVHEGVVTEGSPADLRAGDGVTAKIDAERRWAIMR
                     NHTVTHLTHAALRTVLGPHIKQSGSYVGPDRMRFDFSHHRPMTPEEIREVERLVNEQI
                     LRGTAVQTEIMDIDAARRTGAMALFGEKYGDRVRVVSVPGFSKELCGGTHVQNVAQIG
                     PFFITLETGIASGVRRLEAITGTAAIGYMLDAKQFRSEAAQAVGRSEKDALEGVRQLR
                     ENCAELQRELKRVKAEMFSGSPRAVGAEEQIGAVTLATHDFGDTDRDIMAGWIDRQKD
                     RPDPVVAVALGKVDGKQVYVASASSEAVSRHRVNIGALSKEILPQFGGRGGGKPTFAQ
                     GSVADEAEAARVFAAVRASLTAAQRR"
     gene            1471..2238
                     /locus_tag="PKM94_05750"
     CDS             1471..2238
                     /locus_tag="PKM94_05750"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOD66180.1"
                     /translation="MVEGKVYRRLMEIRADRGGVFLLLIDPDRTSARHNRRLCEAAAE
                     CGVDALLVGTSFMLDTHFAEAVREVKQAAALPVIIFPGSFAQLTPDADAVLFTSLISG
                     RNPEYLIGEQARGAPLVKRFGLEAIPTGYLLIESNNLTSVQYISGTLPIPAAKADIAC
                     AHALAAQYLGMRLVYLDAGSGAHEAVPCAMVREVSAYVEIPVIAGGGLCRPEDCAARI
                     EAGAAMVVVGNSIETDPSFGRLREMAAAVHAKESVRS"
     gene            2235..2825
                     /locus_tag="PKM94_05755"
     CDS             2235..2825
                     /locus_tag="PKM94_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOD66181.1"
                     /translation="MKRENRLAELRDLAAQTARLRLEVVEQLGPLLLDLAARVAGVIG
                     AGGKLLIAGNGGSAADAAHFAAEMVVRLTAARSRQALPALALTADTSVLTAAGNDFGF
                     ENVFARQVEGLGHKGDMLLVISTSGNSENLVRAVGAARQRRMLTAGLLGGQGGRLAGL
                     VDPALIVPSASTQRIQEEHIFLIHLLVEYIESDLFG"
     gene            2822..4090
                     /locus_tag="PKM94_05760"
     CDS             2822..4090
                     /locus_tag="PKM94_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD66182.1"
                     /translation="MSAPATTLADRVLFGLFALFLLSSSFSIALAQSSLGLALAVYIG
                     ILAVRRENPFGGALRPVYLAIGLYVLWVLISALAGPTPGRSVLREREDWLFLIIPIAV
                     HLVRIEPFRRRLVRTLAIAVLLMGAYGVVQHFTGLNWPHATPPLKALEGGYIVQGSFA
                     HRLTYGNYFAVASIFVLACGILAAGRETGVWRRFLFLAAFVGLAATVLSYARMALAGL
                     PLALLFLAALKGRRWLAAAAGAVAIGAVAVYLWVPGVAGGFRAALDRDLTGENQASRT
                     YIWEKSLAIVAENPITGVGLGNFYEAYARQNDSLRGENRVWPHAHNDVLNVAAVAGIP
                     GALLFVAMWVIVFWYLRRGWQRFRGDPAYRALIGAAMAASVMFALCSLSEAAFADEEP
                     RQLLMGVWGMGLGWVNRPSEETPTAAGSAE"
     gene            4162..4425
                     /locus_tag="PKM94_05765"
     CDS             4162..4425
                     /locus_tag="PKM94_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66183.1"
                     /translation="MAKQAKTTASQPATTETRWPFGKRNYIMLAVALAVIIIGYIALG
                     MGSITLAPLLLVVGYCVLIPVALIIKDPSRTRAAGSDDTPPAP"
     gene            complement(4522..7023)
                     /locus_tag="PKM94_05770"
     CDS             complement(4522..7023)
                     /locus_tag="PKM94_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD66184.1"
                     /translation="MKAIVMAGGFGTRLRPLTINIPKPMAPIGNLPMMEHVVSLLVRH
                     GITDVTALLYFQPEKIRNHFKNGAAFGVNMEYAQPDDDYGTAGAVRYALTTATEPVLV
                     ISGDLITDFDLTEAIAWHAARRSQATLLLTRMENPLAYGIVITDDDGRIVRFLEKPSW
                     GEAFSDTINTGIYILEPAAIEMIPPRVNFDFSQNLFPLMLSRQMGLFGKIMAGYWKDV
                     GNVGEYRRVHADFFNGTLNLDLKRPVQPLPSGELYAGENVRIADNVQLSGRVVLGHDA
                     KIEAGARLHNCVIGDRTRIGPGAKLSNTVVWHDANIGAESLVAESVICNSARLGRNVQ
                     FMDDVIVADDTVIGDRATVKANCKIWPGKTVDSGAIVSTSIVWGDRWNRELFTNSKIT
                     GLALTEITPEMTVKVGAAFGAFLGKGAAVLTSRDASDGSRLLKRGLMSGLLAAGVNVA
                     DLETQPIPVVRYGLTRGSYAAGIYVRHSPADFRLIDAIFFDGSGLDMPTAKLKKVERM
                     YFGEDFERASLGDIGHLDQPQHVLEDYREDFLKSLDIDLIRKAGFKVVIDHSNGSSSQ
                     IFPTLSSELGVSAVELNANLNPRKFSTSPEEMAQAMVQLSAIVSSLKADIGFLLNPAA
                     EKLTVVDELGQPVDPQLLLLIVLDLFLQLNKVRRVAVPVAASMGVEEIARDRGAEVIR
                     VANDHLSMMEVMRRGEADFVGGTRGGFIFPGFQSGADAMFATCKILEMMAATRSRFGA
                     LRRQFEDLQRETISVPCPWSKKGTVMRHLIVNSQTRERQLVDGVRIRENGGWVLLIPD
                     RETASFNIMAETRSREATQALVNRYRSLVEEWQTE"
     gene            complement(7043..7714)
                     /locus_tag="PKM94_05775"
     CDS             complement(7043..7714)
                     /locus_tag="PKM94_05775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HOD66185.1"
                     /translation="MTRPAVFCDFDGTIAVRDVGYSIFHRFSGGRNLALLPDWKAGRM
                     SSREILRREAAMAPVTESELLEFLEDFTLDPGFAAFADRCHRQEIDLVVVSDGLDLYI
                     NLLLARNRLSHLPLIANHGYVRDGHIEIEFPFDNRDCARCGSCKGERIREYRLSHPGA
                     GPAVFVGDGFSDACAAHEADLLFAKKDLERYCRMHNIPFVPYQDFFDVARELERRELL
                     SRNDV"
     gene            complement(7717..8010)
                     /locus_tag="PKM94_05780"
     CDS             complement(7717..8010)
                     /locus_tag="PKM94_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66186.1"
                     /translation="MREHYFRTDIVVRLQRWFWWTIVLLFGAATALWAVDAGRARWVT
                     TWGINVVFLIVAFIIVLMAELFRRARLYRYALLSYLLLVILLSTIALKLYVTG"
     gene            complement(8007..8777)
                     /locus_tag="PKM94_05785"
     CDS             complement(8007..8777)
                     /locus_tag="PKM94_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD66187.1"
                     /translation="MPPELVWYIVCGMTAGLLGGYLGLGGGEIVVPFLTVVLGLDVKT
                     AAPVSLVAVTVNALSSSAEYLKRGMVDLELAIIIATFMVMGNIAGSTVSAAVSEELVR
                     VLLTGVLVYTAVTLLRGRRPAERLVFADNRRRFMGLVVVLAFLIGVLAGLIGVGGGVL
                     LIPLMYLVIGCPLATARGTTTLIVVFSAAAASAVYFLRGAVALPVAAPVVFGILLGGR
                     AGAYFGASARPAVVRALFFAVMMYLAWRLSARALEGLW"
     gene            complement(8779..10353)
                     /locus_tag="PKM94_05790"
     CDS             complement(8779..10353)
                     /locus_tag="PKM94_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD66188.1"
                     /translation="MMPSAAPDRVDLLISMVVIVLLVALAGGMGVYVVSDPGLRAAAP
                     LVRAAEVIRSRWVEPVDGARLTEAGRRAMFDLLDRYSGYVDRTDFDRMREELSGAYGG
                     IGVTIVPHDGGLLIMSVREDGPAGRVGLLTGDILVGTDSVPFARLTPAEASALLRGRP
                     GTDVRVRVYRPVAGDTITVALTRQRIPLVHVPFAGLTDRNIIYIRVLDFDAGAADDIR
                     AAVDSLLERAENRPAGLILDLRNNPGGLFSEGYRTANIFLKPGAFIVGTDARSRWNEE
                     VHRAMYRDVTDGLPMAVLVNRGSASSAEIVAGAFQQAGRAVLVGDTTFGKGLVQGFIS
                     FPDGDGLRLTMSRYYFDNGVYLNRLDGSLADSGTGLVPDYYYAYPELDPFVQKLENSL
                     LLQEFAYAHLEELAAEDDLDPAGPLLSAFGRFAAARGFAFTSATTAAARELLRRTEEE
                     AAAPPAGKAAAEALRLAEQQDRALVFSYAGYIAQRLRQIAWERAHGAARTYREVILRS
                     RGDIRLAEEILLARRS"
     gene            complement(10331..10978)
                     /gene="tmk"
                     /locus_tag="PKM94_05795"
     CDS             complement(10331..10978)
                     /gene="tmk"
                     /locus_tag="PKM94_05795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOD66189.1"
                     /translation="MSKNRRGLFISFEGIDGCGKTTQVMLVYKYLIAQGRQVKLVREP
                     GSTEAAERIRALLLDRRLAIDERTELLLYEAARADLAAREIAPALARGLVVLCDRFYD
                     STTAYQGFGRRLDVAMVRALHRVAVDDLEPDLTLVFDCDIRTAMGRRAGDPDRLEAES
                     RAFFERVRKGFLEIARKERRRVKVIDAAQPVETVFADVKKHLVRKLRNHDAERRP"
     gene            complement(11061..12284)
                     /locus_tag="PKM94_05800"
     CDS             complement(11061..12284)
                     /locus_tag="PKM94_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOD66190.1"
                     /translation="MGKIRVAVIGVGNCASSLIQGVEFYKKAREDEKVPGLMHVNLGG
                     YHISDIEFVAAIDIDKNKVGKDLAEAIYTWPNNTYQFCKVPKTGIIVQRGMTHDGLGF
                     YLSQIIEKAPGDTVDIVKLLKDTKTDVVVNYLPVGSEEATKWYVEQILLAGCGFINCI
                     PVFIAKEQYWQKRFKEKGLPVIGDDIKSQVGATIIHRIMTRLFMDRGVKLERTSQLNV
                     GGNTDFLNMLERSRLQSKKISKTSAVTSMLDYDIGAKNVYIGPSDYVEWLDDRKWAHI
                     RLEGTTFGNVPLNVEMKLEVWDSPNSAGVVIDAIRCCKLGLDNGLSGALIAPSAYFKK
                     SPPVQYPDDVARQMVEQFIAEYGWKQKTTAVRPGVRAAAKDRKTARPATGARPAGGGK
                     KPAAAVPKRIIRKKK"
     gene            12454..13131
                     /locus_tag="PKM94_05805"
     CDS             12454..13131
                     /locus_tag="PKM94_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD66191.1"
                     /translation="MTERQRPAPDRLDENEVLRLVRRGAATDTFRRLPAEKKAFIYRT
                     ALRLFGEFGYDGLAVDRFCREAGISKGSFFQYFPSKSHLLEFCVLLFDRSLGEWLADI
                     RRTDGGGLARQRLLHLYRAAAVTSRLHPTEERFYLFVTRALNHAAVALEGIDCERHFS
                     DYVAEIIARGEQTGEIRRDFESALTGRLVALILGALVERAFAGRALPPREAERCVISL
                     LFDGLKA"
     gene            complement(13128..13472)
                     /locus_tag="PKM94_05810"
     CDS             complement(13128..13472)
                     /locus_tag="PKM94_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOD66192.1"
                     /translation="MEPTITFEDFARVDIRVGRIIEVADFSRARNPSYKLRIDFGPEI
                     GIRQSSAQFKQNYAPEALRGTQCLAVVNFPPRNIAGFMSEVLVLGVPRADGNGLAIVR
                     PADDAALGGRLH"
     gene            complement(13533..14468)
                     /locus_tag="PKM94_05815"
     CDS             complement(13533..14468)
                     /locus_tag="PKM94_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD66193.1"
                     /translation="MITALGNPVYDLITTRKVRPTGRVLSGCSTNAALALAKLGERVR
                     LVGAVGEDFRPQLAADLARHGIDHVIIPSAESGGFALNYYDDHGNRTLDLIGRAAPID
                     PIDPAWFADAEAVLVGPILGEISLDNIRRIRRLYDGFFFCDPQGLLRGADAGGRIYHE
                     KAPGIDDILGLFDVVKPNELEARVLTGVDCREDPYRAARLIQKAGPRVVIVTLAELGS
                     VIYDGREFIDIPPYTDIALLDSTGAGDTYMAGFTFEYLRTRDLRRAGCFASCVSSFMI
                     EHTGPDFPLAERAVRARQEELLALADYRVRVAVNR"
     gene            complement(14505..15155)
                     /locus_tag="PKM94_05820"
     CDS             complement(14505..15155)
                     /locus_tag="PKM94_05820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOD66194.1"
                     /translation="MKSIEGTKAAPGLNQRKRQFYEEWSLSLGRVCVRLGFKPNYITA
                     VSLLCAIISGVFFWRGQWLWGVVWMVITSFTDMLDGSTARAGKMGTVFGGILDHVSDR
                     YGEFFILAGITLSGRVAPGWGLFALFGMIIASYTRAAAESMGKIENCAVGIMGRLEKF
                     ILIIIGSLLEYFLPVGAFPRSGWLELALIVVGATSYITSVQRMVYTYKVLGDKREV"
     gene            15402..18020
                     /locus_tag="PKM94_05825"
     CDS             15402..18020
                     /locus_tag="PKM94_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66195.1"
                     /translation="MKRLGVLIVGVLACWIGPDPARARMTEAADGLRGYRRPLAAVSP
                     AHMLAAHRAGRIELAVANNGTFGKEYHPGEASDAFTGEAIPLSCQYPKGSLVAYLFGG
                     AFWIGAVVGRDTLVSVGADGWQQVYEFFPDPAPFGNMVRRSIRDPEDSLAVSEEDYIS
                     VYTDTFPELVNPDPFAAVHTPLCVQVTQASYAWSYEYTEDFVLFDYRIKNIGRETLRQ
                     VYMGIYVDDMICYDCFGSNLGYTDDHSGFLHTYPVRYGRCEYLDTVFLAWQADNDGDL
                     ETIFSDGRRHPCPDVIATRIIRTPAEDLDVSFNWWIGNSNAQLDFGPREREFVGRWKE
                     PVRDFGTGGLGTPEGDRNKYYVLRNQEFDYDQIFTATISSSDSRWLYPDPSLAADYAD
                     GYDTRYLLSFGPFDIQPGRSLPVSFAYMAGEHLHRSPDNAAHLPARPDLYYANLDFSD
                     LALNAGWASRVYDNPGVDTDGDGYRGRFRLMKTRADGTVECLPDGIYIGPPGDSLQGG
                     EPRLDTVWYQGDGVPDFRGAAPPPPPEFRLASPSAGTILVRFNGLLSETAQDQFSGLY
                     DFEGYRVYLGRDDREASFSLAASYDREDFNKLVWDADASEWRLFDPPYTLAELRCLYA
                     DSCGDATFDPLRYTRVNRFVHPRYPDSLFCFEKQDYNAAGSGDLGAIVKSYPDQPYPS
                     SPDPALVRPDELTAEGRLKYFEYEFTITGLLPSVPYWVNVTAFDFGSPSTGLPALESS
                     VTVGAQSIYTVLGGTEAGERLRVIAYPNPYRADGGYRGNGFEGRLAPDRPEDRVRAVH
                     FANLPARCTIRILTLDGDLVREIVHDQPPGSARANHDQWDLITRNTQTVVSGLYYWTV
                     EDGQGNVQLGKLAIIM"
     gene            complement(18058..20433)
                     /locus_tag="PKM94_05830"
     CDS             complement(18058..20433)
                     /locus_tag="PKM94_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD66196.1"
                     /translation="MKYSIVCGLFPALLAGAAAASVIHVPGDHATIQAGIAAAAAGDT
                     VQVAPGTYLENISLAGIDIVLLGAGMDSTILKPADGGLPLVRIHQGETRAAVVRGFSF
                     EESGMSSALVIEGSSPTICFNRITRCPNLSNTPAVLSADSASAPAIHHNLFLRNDGYY
                     QIKAAGPIWIYNNTIYDSRYIGISASHDSAAILNNIIIDCQHRALITSLARIDYNLVL
                     YDIYCCLPYAEHDLLADPLWCDSAAGDLRLWIGSPLIDAGHPDYRDPDGTPTDLGAFS
                     VAGAPPAAWQIAVDSGTLVPTIAWQYADTSGLTQTGYEIEIGRDRDWTAAELWASGPV
                     PSGIPAAAYGGGPLADRGLYYYRLRLQNAEGWGSWREGLFAVQIAPHRKIAVPADLPT
                     IQEAIFAAADGDTIAVAAGTYTGPFNRDLNFLGKQVVLYAPSGPDSTILNLGGDAGLQ
                     RHAFTFRNDEGPTTEVVGFTITNGLGFIRPYSQSGVIKCEGTGPTFRNCRIVDNAGSG
                     IMFSAELGDTLRVIGCDISRNSWEGIGGGTSGVIAVDSTSFLDNSADGIQVGWGTVLR
                     ADRSLFVGNAQRGIFAWYEMVPRGLYVTNCTFADNGTGLWYDWDFPKDGAPAPAVLDS
                     GLSRIIGNVFAFNHGDGLHGSAPCPYFVTCNDWFGNGAADVVIFTDDPIDSANNNSVD
                     PLFCDTAAGDYRVAAGSVCLPENNACGAVIGARGLGCEGGCCAHMGDFQHNGAVTIAD
                     LTAFVAHLFRGGPAPLCFEEVDFNRDAQLTVVDLTLLVAAMFRGAPWPVCP"
     gene            20566..21552
                     /gene="obgE"
                     /locus_tag="PKM94_05835"
     CDS             20566..21552
                     /gene="obgE"
                     /locus_tag="PKM94_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOD66197.1"
                     /translation="MFVDYAEIEVTAGKGGHGVVSFHREKFIPKGGPDGGDGGHGGSV
                     AAVADPQLTTLLDYRYKKHYRAENGQPGQGSLKTGRSGHDIELRLPVGTIVTDLDTGE
                     QLADLDSPGARVILARGGKGGLGNNHFKTSTNQTPRKATTGYPGEHRRLALELKLLAD
                     VGLVGKPNAGKSTILATFSAARPKIADYPFTTLIPNLGIVRLREHKSCVMADIPGLIE
                     GAAEGKGLGLQFLRHIQRTVVLVYVVDINDGDIEGTLRLLRRELARYDESLARRPSLV
                     AVTKIDTVSESDLKAVSEKLSDGYIYLSAVAGLGAGSFLEAIERILDQQRAT"
     gene            21530..22645
                     /gene="dprA"
                     /locus_tag="PKM94_05840"
     CDS             21530..22645
                     /gene="dprA"
                     /locus_tag="PKM94_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD66198.1"
                     /translation="MTSSERHRRLVDTIGLLQVPGIGGGRFDRLVRAFGSAGAASAAS
                     IAELAAVPGISRALATAIHEQYDEASAEATAGQIAQLGWAVIFPDDADYPPMLSALEP
                     PPPLLFRVGEPAHASDRMIAIVGTRHPTERGRMFAHGLARALAESGIAVVSGMAEGID
                     TAAHTGALDGGGRTVAVWGSSLDQVYPPTNRGLADRIRERGAIYSEYLPGTFPDRPHF
                     PERNRIISGMAWGVVVVEAGDRSGALITAEHALRQNRTVFAVPGPPGATASLGCNELL
                     KQGAGLVTSVEDIFRELPTLRGEVKVQRAAERPDLTPTEREIVALFTAGPVQLDDLAR
                     RLNKPVAELTQFLLALELKGVVLELAGKRFTLSDEFA"
     gene            22642..22914
                     /locus_tag="PKM94_05845"
     CDS             22642..22914
                     /locus_tag="PKM94_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOD66199.1"
                     /translation="MITATATIVNKLGLHARPSAMLVTTASRFESEVWVTKDGLRVNG
                     KSIMGVMMLAAERGSQLLIEVNGPDEQEALQAVLRVIEGGFGERPD"
     gene            22976..24751
                     /gene="ptsP"
                     /locus_tag="PKM94_05850"
     CDS             22976..24751
                     /gene="ptsP"
                     /locus_tag="PKM94_05850"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOD66200.1"
                     /translation="MIGMDGKEYTIDGVPISSGIVLGTARVMLPGEAQVSEMPIPASR
                     VPREIDALQKAVDETLDELREVHQAAIRKIGGPVAKIFEAQVMIASDTEFLKRVRAAI
                     EEQRLGAAYVYQKLVQETVDPLQNSPDRYMRQVALDIEAVAARVVARLSGRIPQQKRF
                     APNTIMVGKSFSPNDVVSYRERKATGFVISEGGADSHMALIARALLLPVVLAEEAWQK
                     IPNNTRIIADAVSGRVIVNPSQATWEDYQRRRRRLGPTVLSRIKKLGRIPPVTADGVE
                     VAIGANLTLPGPADEILAHQRVQVGLYRTEFLYLANGAFPSEEAQYDYYRRIARTFGP
                     TPVVLRTFDLGGDKVFNGGGFPVEDNPALGLRGIRTMLAMREEFRTQIRAILRASAHG
                     NLRILLPMVTELEEIERARKIISQEKQKLRREAIPFGEDVPLGIMIEVPSAALTADTL
                     SRRVDFVSIGTNDLTQYTLAADRMNSHVAHLYNALHPAVLNLVWHTVKTCRANGVPVT
                     VCGELAGQRLALPLLIGMGVDVLSMAPARLVEVCRLIRKINSTRARDLVGPVLAGGTL
                     QAVVDRLRDYRETLEHVDPRMREDS"
     gene            24754..25830
                     /locus_tag="PKM94_05855"
     CDS             24754..25830
                     /locus_tag="PKM94_05855"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOD66201.1"
                     /translation="MNILDDIEKIRAHDPENMYNRIFDFPEQLADAVKLGKLWKFDAS
                     AFPGIKNIVTVGMGGSGIAGDLMRSYLRTKLLVPVDICRSYALPEYVDDETLVVAASY
                     SGNTEETLAAVDDALNRKAMLAAIATGGLLEEVAQLNDIPLAKLPGGLQPRAALGYLF
                     VMTMMFLEQVGHAKGFSKELEKVIPQLQKHREKYIEDNSTTINPAKNLAQKLHKKIVI
                     VYSGPTLTDAVGRRWKAQLCENAKTLAFANQFPEFNHNELVGWSPSIEELASHLAVVI
                     LRDLGDHPQVRQRMNIVGELIRSRGVEAIDVHSRGETELERMLSLVQFGDFVSYYLAI
                     LNEVDPTPVKIIDALKKALAEAKK"
     gene            26021..26221
                     /locus_tag="PKM94_05860"
     CDS             26021..26221
                     /locus_tag="PKM94_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOD66202.1"
                     /translation="MITPETRVDEMVERYPDAVRFLMSRGLPCVVCGEPFWGSLEELC
                     RQKGWPTVRLAELVDEFNRLHS"
     gene            26544..26918
                     /locus_tag="PKM94_05865"
     CDS             26544..26918
                     /locus_tag="PKM94_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66203.1"
                     /translation="MKRLLTVSVVLVLLLATAVSSVSARRNPIAVPYPRPSSEDHPWG
                     GEQNYFPDSRLSQPGTSSGVAPGIVGIETSLLWRLVMPLWFERLTGHTIVTLPQPSTD
                     GNAIVGENNPGATAPANNGRGN"
     gene            26931..29174
                     /locus_tag="PKM94_05870"
     CDS             26931..29174
                     /locus_tag="PKM94_05870"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD66204.1"
                     /translation="MRVITDTADGLLAAAAHYLEELLAARDFSGAVKYYEANRRQLET
                     AGSALAGRILRLVARAHLALASYPTALRLARMAQAIHSEHGDTLELAETFLTVAEVLR
                     DSGELKQAEKAFRDAESIFRRNDCAEGQSRALNLLAGLYFRQNDYRNSLTVLMEAIGL
                     AKRLDDRKKLAYMMGNIGRIQTFLGEFAEAKKHLEINLTLSEELGQTLEAARAALSLG
                     YVQMQEGDFAGAETTFQKAAAGLRRVESPRDQVILRTYLGELYYRSERAAEAAEMLES
                     ALAAAEAIGRETTLTGRVLRHLAEASLRVGHDRVAARYAARALAILAKTGEMVEQGAV
                     LKVQAILAERGGRIAEARELFVKALDTLHQSGVRFEKAEALVAAGQSAAFDPRKRLTC
                     LFRAEEYFRRGGMQRRLEEIDRLLAGTDRLRPGDAPGAAARTSEVEFLTANEQIKRFK
                     QQLSYVAKSDLPVLLTGETGVGKDHMARYFHAICRPDGPFVAVNCASIPETLLESELF
                     GYAKGAFTGADRDKEGLFVTANGGVLYLDEIGDMPPTLQAKLLGVLESRRVVPVGSTK
                     EVPLDIRLVVATNRDLQAMVERGLFRRDLYYRISGITFHLPPLRDRKEDIPLLLRHFL
                     QNCRRLAESPLPADVIHRFVEYAWPGNVRELNNKVKRLEVMAELAAEGDLSELAHAVF
                     GDPGRDQRSSLFEQVERFERQMLIEALLATGGNKSEAARILGIHEATVRTKLKRYGIS
                     LEGGALN"
     gene            29783..29950
                     /locus_tag="PKM94_05875"
     CDS             29783..29950
                     /locus_tag="PKM94_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66205.1"
                     /translation="MSTFEMVRQRIRWYHVAGLAVLVVVTAGLVWFWLERRRTAALRA
                     EDKTDLAGGAE"
     gene            30078..>30363
                     /locus_tag="PKM94_05880"
     CDS             30078..>30363
                     /locus_tag="PKM94_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD66206.1"
                     /translation="MALLTDKVAVVTGASRGVGAATARLLAAEGARVVLCARRREGLE
                     QAAHTLDPDGSRCLIAPADVGTLDGMKGLVDAALGRFGRIDLFINNAGAGV"
BASE COUNT         5165 a         9739 c        10432 g         5027 t
ORIGIN      
        1 cgacctgacc gagatcatcg ccgccgagcg cggcctgcgg ctggaccggg aagggttcga
       61 ccgggcgatg gagcggcagc gcacgcagtc gaaggcgggg gcgcgcttcg ccgcgcaagt
      121 gtcgtcgctc aacgaggttc tcgacgccgc cggtctgcgc gcggcgccga ctgtgttcgc
      181 gcgcgaccgg ttcgaggcgg ccgcctccat ccagcacgtc ttcccctggc cgggcggcga
      241 tccccggcgg gtcgggatca tcctcgacac cacgccgttc tacaccgagg ccggcggcca
      301 ggtggacgat accggcgtga tcgtcggccc gggcgcggcc gtgcgggtcg aggccgtgca
      361 cggctaccgg gactggtcgg tgcacgaagg ggtggtgacc gagggctcgc cggccgacct
      421 gcgggccgga gacggggtta cggcgaaaat cgacgccgag cggcgctggg ccatcatgcg
      481 caaccacacc gtcacccacc tcacccacgc cgcgctgcgg acggtgctcg gcccgcacat
      541 caaacagtcg ggatcgtacg tcggaccgga ccgcatgcgc tttgacttct cccaccaccg
      601 gccgatgacg ccggaagaga tccgcgaggt cgaacggctg gtcaacgagc agatccttcg
      661 cggcacggcg gtgcagaccg agatcatgga tatcgacgcg gcccggcgga cgggagcgat
      721 ggcgctgttc ggagagaaat acggcgaccg ggtccgggtg gtgtcggtcc cgggattctc
      781 caaggagctg tgcggcggca cccacgtgca gaacgtcgcc cagatcggac cgttcttcat
      841 caccctggag acgggcattg cctcgggcgt gcggcgcctc gaagcgatca ccggcacggc
      901 cgcgatcggc tacatgctcg atgccaagca gttccgcagc gaggcggccc aggcggtcgg
      961 gcggagcgaa aaagacgccc tcgagggcgt gcggcagctt cgcgaaaact gcgccgaact
     1021 gcagcgcgaa ctgaagcggg tgaaggcgga gatgttctcc ggctcgccgc gcgcggtggg
     1081 cgcggaggag cagatcggcg ccgtgacgct ggccacccac gatttcggcg acaccgaccg
     1141 cgacatcatg gccgggtgga tcgaccgtca gaaagaccgg cccgatccgg tggtcgcggt
     1201 ggcgctgggg aaagtcgacg gcaagcaggt gtacgtggcg tcggcctcga gcgaggcggt
     1261 cagccggcac cgggtgaaca tcggggcgct ctccaaagag atcctgccgc agttcggagg
     1321 ccggggcggc ggcaaaccga ctttcgccca ggggagcgtg gccgacgaag ccgaggcggc
     1381 ccgggtgttc gcggcggtgc gggcgagcct caccgcggcg cagcgccggt agcggccctc
     1441 accgcgggcg cgccggctta gggacacgag atggttgaag gcaaagtata ccgcaggctt
     1501 atggagatcc gggccgaccg cggcggggtg tttctcctgc tgatcgaccc ggaccgcacc
     1561 tcggcgcggc acaaccgccg gctctgcgag gcggccgccg agtgcggcgt cgacgccctg
     1621 ctggtgggga cctcgttcat gctcgacacc catttcgccg aggcggtccg cgaggtcaaa
     1681 caggcggccg cgctcccggt gatcattttc cccgggtcgt ttgcgcaact gacgcccgat
     1741 gcggacgcgg tgctgttcac ctcgctcatc tccggccgca acccggagta tctgatcggg
     1801 gagcaggcgc gcggggcgcc gctggtcaag cgcttcggcc tcgaggccat ccccaccggc
     1861 tacctgctca tcgaatcgaa caacctgacc tcggtgcagt acatctcggg gacgctgccg
     1921 atcccggccg ccaaggccga tatcgcgtgc gcgcacgcgc tggcggccca gtacctcggc
     1981 atgcggctgg tctatctcga cgccgggagc ggggcgcacg aggcggtgcc gtgcgcgatg
     2041 gtccgcgagg tgagcgccta tgtcgagatc ccggtgatcg cggggggcgg gctgtgccgg
     2101 ccggaggact gcgcggcgcg gatcgaggcg ggcgcggcaa tggtggtggt gggaaattcg
     2161 attgaaaccg acccgtcgtt cgggcgcctg cgggagatgg cggcggcggt gcatgcgaag
     2221 gagtccgtgc gatcatgaaa cgggaaaatc gtctggccga actgcgcgac ctggccgccc
     2281 agaccgcgcg gctccgcctt gaggtcgtcg aacaactcgg ccccctcctg ctcgacctgg
     2341 ccgcccgcgt cgccggcgtg atcggcgcgg ggggcaagct gctgatcgcc gggaacggcg
     2401 gctcggcggc cgatgccgcg cacttcgcgg ccgagatggt ggtccgattg acggcggccc
     2461 ggagccgcca ggccctgccg gcgctcgcgc tcaccgcgga cacatcggtc ctgacggccg
     2521 cggggaacga tttcggcttc gagaacgtgt tcgcccgcca ggtggaaggg ctaggccaca
     2581 agggagacat gctcctggtg atctcgacct cgggcaattc ggagaatctc gtgcgggcag
     2641 tcggcgctgc gcgccaacgg agaatgctga ccgcggggct gctcggcggc caggggggac
     2701 ggctggccgg actggtggac cctgccctga tcgtgccgag cgcgtcgacc cagcggatcc
     2761 aggaggagca tatcttcctc atccacctgc tcgtcgagta catagaaagc gacctgttcg
     2821 gatgagcgcg ccggcgacca cactcgccga ccgcgtgctc ttcggcttgt tcgcgctctt
     2881 ccttctgagt tcaagtttct cgatcgcgct ggcccagagc agcctcggcc tggcgctggc
     2941 cgtctatatc ggcatcctcg ccgtgcgccg ggaaaatccg ttcggcggcg cgctccggcc
     3001 ggtctacctg gcgatcgggc tgtatgttct ctgggtgctg atctcggcgc tggcgggacc
     3061 gacccccggc cggtcggtgc tgcgggagcg ggaggactgg ctcttcctca tcattccgat
     3121 cgccgtccac ctcgtgcgca tcgagccgtt ccgccggcgg ctggtgcgga cgctcgccat
     3181 cgccgtgctg ctgatgggcg cgtacggcgt cgtgcagcac ttcaccgggc tcaactggcc
     3241 gcacgcgacg ccgccgctca aagcgctcga gggagggtac atcgtgcagg ggtcatttgc
     3301 gcaccgcctc acctacggca actatttcgc ggtggcctca atcttcgtgc tggcctgcgg
     3361 aatactggcc gccgggcggg agaccggggt gtggcggcga tttcttttcc tcgcggcctt
     3421 cgtgggcctg gcggcgaccg tgctctccta tgcgcggatg gcgcttgccg ggctgccgct
     3481 cgccctgctg ttcctcgcgg ccctcaaagg gcggcggtgg ctggcggcgg ccgcgggcgc
     3541 agtggcgatc ggcgccgtcg ccgtgtacct ctgggtgccc ggggtggccg gggggttccg
     3601 ggcggcgctg gatcgggacc tgaccggcga gaaccaggcg tcgcgcacgt acatctggga
     3661 gaaatcgctc gcgatcgtag cggagaaccc gatcaccggg gtcggcctgg gcaacttcta
     3721 cgaggcctat gcgcggcaga acgattcttt gcgcggtgag aatcgggtgt ggccgcatgc
     3781 gcacaacgac gtcctcaacg tcgcggccgt ggccggaatt cccggcgccc tcctttttgt
     3841 cgccatgtgg gtcatcgtgt tttggtatct ccggcgcggc tggcagcggt tccggggcga
     3901 tcccgcttac cgcgcgctga tcggcgcggc gatggcggcg tcggtcatgt ttgccctctg
     3961 ctcgctgtcg gaggcggcgt ttgccgatga ggagccccgc cagttgctga tgggcgtctg
     4021 ggggatggga ctgggctggg tcaaccggcc gtccgaggag acgccgacgg cggcgggttc
     4081 ggcggagtga gcgggcgggg ccgccccgga aaaccgcttg acaggaacag gtccggcgat
     4141 cttattggaa gtcgacaggt tatggcaaaa caagcaaaaa caacggcatc gcagccggcg
     4201 acgaccgaga cccgttggcc gttcggcaag cgcaactaca tcatgctggc ggtggccctg
     4261 gccgtgatca tcatcggcta catcgctctc ggcatgggct cgatcacgct ggcgcccctg
     4321 ctactcgttg tcggctactg cgtcctgatc ccggtcgcgc tgatcattaa ggatccgtcg
     4381 cggacccgcg cggccggcag cgacgacact ccgccggcgc cctgagcagc ggcggtctcc
     4441 gcggtcggcg ggccgggccg cgccgagagg cggcgctcct gcccgggtcg gcgccgcaca
     4501 tgcctgatgg cgtgtttccc cctattcggt ctgccactcc tccaccaggc tccggtagcg
     4561 gttgacgagc gcctgggtgg cctcgcgcga acgcgtttcc gccatgatgt tgaacgacgc
     4621 cgtctcgcgg tcggggatga gcaggaccca gcccccgttt tctctgattc gcacgccgtc
     4681 gaccaactgc cgctcgcgcg tctgggagtt tacaatcagg tggcgcataa ccgtgccctt
     4741 cttcgaccac gggcagggga cggagatggt ttcgcgctgg aggtcctcga attgtcggcg
     4801 gagggcgccg aagcggctgc gggtcgcagc catcatctca aggatcttgc aggtggcgaa
     4861 catcgcatcg gccccgctct ggaagccggg gaagatgaag ccgccgcggg tgccgccgac
     4921 aaagtccgcc tctccgcgcc gcatcacctc catcatcgag aggtggtcgt tggcgacgcg
     4981 gatgacctcg gcgccgcggt cgcgggcgat ttcctcgacg cccatcgagg ccgccaccgg
     5041 gaccgccacc cgccgcactt tgttcaactg caggaacaga tcgaggacga tgagcaggag
     5101 cagctgcggg tcgaccggct gcccgagttc atcgaccacg gtgagtttct cggccgccgg
     5161 gttgagcagg aagccgatgt cggccttgag ggaggagacg atggcggaga gctgcaccat
     5221 ggcctgggcc atctcctcgg gcgaggtcga gaacttgcgc gggttgaggt tcgcgttcag
     5281 ttcgacggct gagacgccga gctcggagga cagggtcggg aagatctggc tcgaggagcc
     5341 gttggagtgg tcgatgacga ctttgaatcc ggccttgcgg atcaggtcga tgtcgagcga
     5401 tttgaggaaa tcctcgcggt agtcctcgag cacgtgctgg ggctggtcga ggtggccgat
     5461 gtcgccgagc gaggcgcgtt cgaaatcctc gccgaaatac atgcgctcca ccttcttcag
     5521 cttggccgtc ggcatgtcca gcccggagcc gtcgaagaag atcgcgtcaa tcaagcggaa
     5581 atcggccggg gagtggcgca cgtagatccc ggcggcgtaa ctcccccgcg tcagaccgta
     5641 gcgcaccacg ggaatgggct gggtttcgag gtcggcaacg ttgacgcccg cggccaggag
     5701 gcccgacatg agcccgcgtt tgagcagacg cgacccgtcc gaggcgtcgc ggctggtcaa
     5761 cacggccgcg cccttgccga ggaaagcgcc gaaggcggcg ccgactttga ccgtcatctc
     5821 gggcgtgatt tcggtcagcg ccaggccggt gattttcgag ttggtgaaca actcccggtt
     5881 ccagcggtcg ccccagacga ttgaggtgga gacgatggcg ccggaatcga ccgtcttgcc
     5941 cggccagatt ttgcagttgg ctttgaccgt cgcgcggtcg ccgatcaccg tgtcgtcggc
     6001 gacgatgacg tcgtccatga actgcacgtt gcggccgagc cgtgcggagt tgcagatcac
     6061 gctctcggcc accaggctct cggcgccgat gttggcgtcg tgccacacca ccgtgttgct
     6121 gagcttggcg cccgggccga tccgcgtgcg gtcgccgatg acgcaattgt ggagcctggc
     6181 ccccgcctcg atcttcgcat catggccgag gacgacacgc ccggagagct ggacgttgtc
     6241 ggcgatacgg acattctccc cggcgtagag ctcgcccgag gggagcggtt gcaccggccg
     6301 cttgagatcg aggttgagcg tgccgttgaa gaagtcggcg tgcactctcc ggtactcccc
     6361 cacgttgccg acgtctttcc agtagccggc catgatctta ccgaacagcc ccatctgccg
     6421 cgagagcatg agggggaaga gattctgcga gaagtcgaaa ttgacccggg gcgggatcat
     6481 ctcgatggcc gcgggctcga gaatgtagat gccggtgttg atggtatcgg agaaggcctc
     6541 gccccacgag ggtttctcca ggaagcgcac gatccgaccg tcgtcgtcgg tgatcacgat
     6601 tccgtaggcg agtggattct ccatgcgggt gagcaggagc gtcgcctgcg agcgccgggc
     6661 ggcgtgccag gcgatagcct cggtgagatc gaaatcggtg atcaggtcgc ccgagatcac
     6721 gagcaccggt tccgtggccg tggtgagagc gtagcggacc gcccccgccg tgccgtaatc
     6781 gtcatccggc tgcgcgtact ccatgttcac cccgaacgcc gcgccgttct taaaatgatt
     6841 gcggatcttc tccggctgga agtacagcag cgcggtcacg tcggtgatgc cgtgcctgac
     6901 caggagggac accacgtgtt ccatcatggg caggttgccg atcggggcca tgggtttggg
     6961 tatgttaatg gtcaaagggc gcagtcgggt tccgaaaccg cccgccatga cgatcgcctt
     7021 catgtcgact tctcccttaa gtctaaacgt cgttacgact taacaattcg cgtcgctcca
     7081 gttcgcgagc aacgtcgaag aaatcctgat acggcacgaa tggaatattg tgcatgcggc
     7141 agtagcgttc aaggtctttt ttcgcaaaga gaaggtcggc ctcgtgcgcc gcacaggcgt
     7201 cggaaaatcc gtcgccgaca aagaccgccg gcccggcccc cgggtggctt aagcggtact
     7261 cgcgaatccg ctcccctttg cagctgccgc acctggcgca gtcccggttg tcaaagggga
     7321 attcgatttc gatgtgcccg tcgcgcacat agccgtgatt ggcgatcagc ggaaggtgcg
     7381 acaggcggtt gcgggccaag agcaggttga tgtacaaatc gaggccgtcg gacacgacca
     7441 ccagatcgat ctcctgccga tggcagcggt cggcgaaggc ggcgaaaccg gggtcgagtg
     7501 tgaaatcctc gagaaactca agcagttcgc tctcggtcac cggcgccatc gcggcctcgc
     7561 ggcggaggat ctcccggctc gacatgcgcc ccgctttcca gtcggggagg agagccagat
     7621 tccggccgcc ggagaagcgg tggaaaatgc tgtagccgac atcgcgaacg gcgatggtgc
     7681 cgtcgaagtc gcagaaaacg gccggtcgcg tcatggtcat ccggtaacat acaacttgag
     7741 cgcaatggtc gaaaggagaa tcaccaggag caggtacgac agcagcgcgt agcggtagag
     7801 tcgggcgcgc cggaacagct ccgccatcag gacgatgatg aaggcgacga tgaggaagac
     7861 cacgttgatc ccccaggtcg tgacccatcg ggcgcggccc gcatcgaccg cccacagcgc
     7921 ggtcgcggcg ccgaacagca gcacgattgt ccaccagaac cagcgctgga ggcggacgac
     7981 gatgtcggtg cggaagtagt gttctctcac cagaggccct ccagcgcgcg ggcggagagc
     8041 cgccacgcga ggtacatcat caccgcgaag aagagcgcgc ggacgaccgc cgggcgggcg
     8101 ctggcgccga aataggcgcc ggcccggccg ccgaggagaa tgccgaagac gaccggcgcc
     8161 gccaccggca gcgcgaccgc gccgcgcagg aaatagaccg ccgacgcggc ggccgcggaa
     8221 aagaccacga tgagggtggt cgtcccccgg gctgtcgcca gcgggcagcc gatcacgagg
     8281 tacatcaggg ggatcagcag gacgcctccg ccgacgccga tcagcccggc gagaacgccg
     8341 atgagaaagg cgagcacgac gaccaggccc atgaagcggc ggcggttgtc ggcaaacacc
     8401 agccgctcgg ccgggcggcg gcccctaagc agggtcaccg ccgtatagac caataccccc
     8461 gtcaggagaa cccgcaccag ctcctcggac accgcggcgc tcaccgtgct gccggcgatg
     8521 ttgcccatga ccatgaaggt ggcgatgatg atggccagtt ccagatcaac catgccccgc
     8581 ttgaggtact cggccgacga cgagagcgcg ttcactgtga cggcgaccag cgagaccggg
     8641 gccgcggtct tgacgtcaag cccgaggacg acggtgagga aggggacaac gatttcccct
     8701 ccgccgaggc cgaggtagcc gccgagcagt ccggcggtca tcccgcaaac gatgtaccag
     8761 acgagttcgg gtggcatgtc atgaccgccg ggcgaggagg atctcttcgg ccagtcggat
     8821 gtcgccgcgg gagcggagga tcacttcgcg gtaggttcgc gccgcgccgt gcgcccgttc
     8881 ccaggcgatc tgccggaggc gctgggcgat gtagccggcg taggagaaca ccagggcgcg
     8941 atcctgctgc tccgccaggc ggagcgcctc cgcggcggct ttccccgccg ggggcgccgc
     9001 ggcttcttcc tccgttcgcc ggagcagttc gcgggcggcc gcggtagtgg cgctggtgaa
     9061 cgcgaatccg cgcgcggcgg cgaagcggcc gaacgcggac agcagcggtc ctgccgggtc
     9121 gaggtcatcc tccgccgcca gctcctccag gtgcgcatag gcaaattcct gcaggagcag
     9181 cgaattctcc agtttctgca cgaaggggtc gagttcgggg taggcgtagt agtagtccgg
     9241 aaccaggccg gtgccggaat cggcgaggct cccgtccagc cggttcaggt agaccccgtt
     9301 gtcgaagtag tatcgtgaca tggtgagccg aagcccgtcg ccatccggaa aactgatgaa
     9361 cccctggacg agtcctttgc cgaaggtggt gtcgccgacc agcaccgccc gccccgcctg
     9421 ctggaacgcc ccggcgacaa tctcggccga cgaggcggag ccgcggttga ccaacaccgc
     9481 catcggcaga ccgtcggtga cgtcgcggta catcgcccgg tggacctcct cgttccagcg
     9541 ggagcgggcg tcggtgccga cgataaaggc gccaggtttg aggaagatgt tggccgtccg
     9601 gtagccctcg ctgaacaggc cgccggggtt gttgcgcagg tcgaggatga gccccgccgg
     9661 ccggttctcg gcgcgctcca ggagggaatc gacggcggcg cggatatcgt cggcggcccc
     9721 ggcatcgaaa tcgagcacgc ggatgtagat tatgttgcgg tcggtgaggc cggcgaaggg
     9781 cacgtggacg agcgggatac gttggcgggt gagagcgacc gtgatcgtgt cgccggccac
     9841 cggccggtag acccggacgc gcacgtcggt gccgggccga ccccggagca gggcgctggc
     9901 ctcggccggg gtgagccggg cgaagggcac ggaatcggtg ccgaccagga tgtcgccggt
     9961 gagcagcccg acgcggcccg ccggtccgtc ctcgcgcacc gacatgatca gcagtccgcc
    10021 gtcgtggggg acgatggtca cgccgatgcc gccgtaggca ccggagagtt cctcgcgcat
    10081 gcggtcgaag tcggtgcggt cgacgtagcc cgagtagcgg tcgagcaggt cgaacatggc
    10141 gcggcggccg gcctcggtca ggcgcgcgcc gtcgaccggc tccacccagc gtgagcggat
    10201 cacctcggcg gcccgcacca ggggcgcggc ggcgcgcagg cccgggtcgg acacgacgta
    10261 cacgcccatg ccgccggcga gcgcgaccag cagcacgatg acgaccatcg agatcaacag
    10321 gtcgacccgg tcaggggcgg cgctcggcat catgatttct cagtttgcgc acgaggtgtt
    10381 tcttgacgtc ggcgaacacg gtctccaccg gctgggcggc gtcgatcact ttgacgcggc
    10441 ggcgctcctt gcgcgcgatt tcgaggaacc ccttgcgaac ccgctcgaag aaggcgcgcg
    10501 attcggcctc gagccggtcg gggtcgcccg cccggcggcc catggcggtg cgaatgtcgc
    10561 agtcgaacac cagcgtcagg tcgggttcga gatcgtccac cgccacccgg tgaagggccc
    10621 gcaccatggc gacatccagc cgccggccga aaccctggta ggcggtggtg ctgtcgtaga
    10681 agcggtcgca caagaccacc aggccgcgcg cgagcgccgg ggcgatctcc cgggctgcca
    10741 gatcggcccg cgccgcctcg tagagcagca gctcggtgcg ctcgtcgatg gccaggcggc
    10801 ggtcaaggag gagggcgcgg atccgctcgg ccgcctcggt cgacccgggc tcgcgcacca
    10861 gcttgacctg gcgcccctgc gcgatcaggt atttgtagac gagcatcacc tgggtggtct
    10921 tgccgcaccc gtcgatgccc tcgaaagaga tgaacaatcc gcgtcggttc ttgctcacac
    10981 ggctaccctg acggccacaa aaacataccg gcggtggaac ccaccgccgg taaagtagcc
    11041 tgcgcaaaga gggcgggaaa ctactttttc ttgcggatga tgcgcttggg cacggccgcc
    11101 gccggtttct ttccgccccc agccgggcgg gcgccggtcg cggggcgggc cgtcttgcga
    11161 tccttggcgg cggcgcggac gcccggccgg accgccgtcg tcttctgctt ccagccgtac
    11221 tcggcgatga actgctcgac catctgccgg gccacgtcgt cggggtactg caccggcggg
    11281 gatttcttga agtaggccga gggggcgatg agggcgcccg agaggccgtt gtccaggccg
    11341 agcttacagc agcggatcgc atcgatcacg acccccgccg agttcgggct gtcccagacc
    11401 tcgagtttca tttcgacgtt gagcgggaca ttgccgaacg tcgtgccctc cagccggatg
    11461 tgcgcccact tccggtcgtc gagccactcc acgtagtcgg aggggccgat gtagacgttt
    11521 ttcgcgccga tgtcgtagtc gagcatgctg gtgacggcgc tggtcttgga gatcttcttg
    11581 gactgcaggc gcgaccgctc gagcatgttg aggaagtcgg tgttgccgcc gacgttgagc
    11641 tggctggtgc gctcgagctt gacgccgcgg tccatgaaca gccgggtcat gatgcggtgg
    11701 atgatggtgg cgccgacctg cgacttgatg tcgtcgccga tcaccggcag ccccttctcc
    11761 ttgaaccgct tctgccagta ctgctccttg gcgatgaaga ccgggatgca gttgatgaag
    11821 ccgcagccgg ccaggaggat ctgctcgacg taccacttgg tggcctcctc cgagccgacc
    11881 gggaggtagt tcacgacgac gtcggtcttg gtgtctttca ggagtttgac aatgtcgacg
    11941 gtgtcgccgg gggccttctc gatgatctgc gacaggtaga accccagtcc gtcgtgcgtc
    12001 atgccgcgct ggacgatgat gcccgttttc ggcaccttgc aaaactggta ggtgttgttc
    12061 ggccaggtat agatcgcctc ggcgagatcc ttgccgacct tgttcttgtc gatgtcgatg
    12121 gcggccacga actcgatatc gctgatgtgg tagccgccga ggttgacgtg catgagcccc
    12181 gggaccttct cgtcctcgcg ggccttcttg tagaactcaa cgccctggat gagcgatgag
    12241 gcgcagttgc cgacgccgat gaccgcaact ctgatttttc ccataccatt cttccttcct
    12301 gctctggcgg gccggtgcgg cccgtttgcc tttttcgccg cgaatataga gatcggccgc
    12361 cgcccctgtc aaattgaaag aaaccgcttg ccgtcattat agaacaatag tatatcacag
    12421 gcgaccaaac agtcatatcc tgtggagggc cgtatgaccg agcgacagcg gccggcgccc
    12481 gaccggctcg acgaaaatga agtcctccgg ctggtgcggc gcggcgccgc caccgatact
    12541 ttccgccgcc tgccggccga gaagaaggcg ttcatttacc ggaccgcgct ccggctgttc
    12601 ggggagttcg gttacgacgg tcttgcggtg gaccggttct gccgcgaggc gggaatcagc
    12661 aaaggctcgt tcttccagta cttcccctcc aagagccacc tcctggagtt ctgcgtgctg
    12721 ctcttcgacc gttcgctggg ggagtggctc gcggacatcc ggcgaaccga cgggggcggg
    12781 ctggcccggc agcggctgct gcacctctac cgggcggcgg cggttacctc gcggctgcac
    12841 ccgaccgagg aacggttcta cctgttcgtt acgcgggcgc tcaatcacgc ggccgtggcg
    12901 ctggagggaa tcgactgcga gcggcacttc agcgactacg tggctgagat catcgcgcgg
    12961 ggagagcaga cgggggaaat tcggcgggat ttcgagagcg cgctgacggg gcggctggtg
    13021 gcgctcattc tcggggcgct ggtcgagcgg gcattcgcgg ggcgggccct gccgccgcgg
    13081 gaggcggagc gctgcgtcat ttcgctgctc ttcgacggcc tcaaggccta gtggagccgc
    13141 ccgccgagcg ccgcgtcatc ggccgggcgg acgatcgcga gcccgttgcc gtcagcccgc
    13201 ggcactccca ggacgagcac ctccgacatg aagccggcga tgttgcgcgg cgggaagttg
    13261 accaccgcga gacactgcgt cccgcgcagc gcttcgggcg cataattctg cttgaattgg
    13321 gcggaggact gccggatccc gatttccggg ccgaagtcga tgcggagttt gtaggagggg
    13381 ttgcgggcgc gcgagaagtc ggccacctcg ataatgcggc cgacgcggat gtcgacccgg
    13441 gcaaaatcct cgaacgtaat cgtgggttcc atgctgtctc tccgttgctg cgcgcgcggg
    13501 agcgcgccgc gacacggcgc gcccgccctg ggctagcggt tgacggccac ccgcacgcgg
    13561 tagtcggcca gggccagcag ctcctcctgg cgggcgcgga cggcgcgctc ggcgagcggg
    13621 aaatcggggc cggtgtgctc gatcataaaa gaggagacgc acgaggcaaa acagccggcc
    13681 cgccgaaggt cccgcgtacg caggtactcg aacgtgaagc cggccatgta ggtgtcgccg
    13741 gcgccggtgg aatcgagcag ggcgatgtcc gtgtaagggg ggatgtcgat gaactcccgg
    13801 ccgtcgtaaa tgaccgagcc gagttcggcg agcgtgacga tcaccacccg cggccccgcc
    13861 ttctggatga gccgggccgc ccggtaggga tcctcgcggc agtcgacgcc ggtgagaacg
    13921 cgcgcctcga gctcattggg cttgacgacg tcgaagaggc cgaggatgtc gtcgatgccg
    13981 ggggctttct cgtgatagat gcgcccgccg gcatcggcgc cgcggagcag gccctgcggg
    14041 tcacagaaga agaacccgtc gtagaggcgg cggatgcgcc ggatattatc gagcgaaatc
    14101 tcgccgagga tgggaccgac cagcaccgcc tcggcatcgg caaaccaggc cgggtcgatc
    14161 gggtcgatcg gcgcggcccg gccgatcagg tcgagcgtcc ggttcccgtg gtcgtcgtag
    14221 taattcaggg cgaaaccgcc gctctccgct gacgggatga tgacgtggtc gatcccgtgg
    14281 cgtgcgaggt cggcggccag ctgcggacgg aaatcctcgc cgacggcgcc gacgaggcgg
    14341 acgcgctcgc ccagcttcgc caacgcgagc gcggcgttgg tcgagcagcc ggagagcacg
    14401 cggccggtgg gccggacctt ccgggtcgtg atgaggtcgt agaccgggtt gccgagcgcg
    14461 gtgatcatgg gggaaaaatc ctttccgggc tcccgcggcg gcgtctacac ctcgcgcttg
    14521 tcgcccagca ccttgtaggt gtacaccatc cgttggacgg aggtaatgta cgaggtcgcg
    14581 ccgaccacga tcagcgccag ctccagccag ccgctgcgcg ggaaagcgcc cacggggaga
    14641 aaatactcca gcagcgagcc gatgatgatg aggatgaatt tctcgagccg ccccatgatg
    14701 ccgacggcgc aattctcaat cttgcccatc gactcggccg cggcccgcgt gtacgaggcg
    14761 atgatcatgc cgaaaagggc gaacagaccc caccccgggg ccacccggcc ggagagtgtg
    14821 atgccggcga gaatgaagaa ctcgccgtag cggtcggaga cgtggtcgag gatgccgccg
    14881 aagacagtgc ccattttccc ggcgcgggcc gtcgagccgt caagcatgtc ggtgaacgac
    14941 gtgatgacca tccacaccac cccccacaac cactgccccc gccagaaaaa gacgccggag
    15001 atgatcgcgc agaggaggct gacggcggtg atgtagttgg gtttgaagcc gagccggacg
    15061 cacacccgcc cgagcgagag cgaccactct tcgtaaaact gccgtttgcg ctggttgagt
    15121 cccggcgccg ctttcgtccc ttcgatgctt ttcatcccgg cgagtaatat atgcgctttt
    15181 tgggggaggt caacgagtca acccggcgcg cgcggcggtt tttttgggcc cgtgtccgcc
    15241 ccgcggggtg ctccccgccc gcaaaaacca tcttgcccgg caagggctta gcgcggccgc
    15301 ggaaaaccgc ttggccaggc ggcgttgaac tgttttcgtt ggagtagacg gccggcggcg
    15361 gtcgcatacg tattccgccc gccgccgcgc ggcgtagttc catgaaacga ctgggagtcc
    15421 tgatcgtggg cgtgctcgcc tgctggatcg gcccggatcc ggcgcgggcc cgcatgacgg
    15481 aagccgcgga cggcctccgc gggtaccgcc gtccgcttgc ggccgtctcg ccagcccaca
    15541 tgctcgccgc ccaccgggcc ggccgcatcg aactggccgt cgccaacaac ggcaccttcg
    15601 gcaaggagta ccatcccggc gaagcctccg acgctttcac cggcgaggcc atcccgctct
    15661 cctgccagta ccccaaaggt tcgctggtcg cctacctctt cggcggcgcc ttctggatcg
    15721 gcgccgtggt cggacgcgat accctcgtgt cggtgggcgc cgacggctgg cagcaggtgt
    15781 acgagttctt ccccgacccc gcccccttcg gcaacatggt ccgccgctcc atccgcgatc
    15841 ccgaagactc cctggccgtc tccgaggaag actacatctc ggtttacacc gataccttcc
    15901 cggaactggt caaccccgat ccgttcgccg ccgtccacac cccactttgc gtgcaggtga
    15961 cgcaggcgtc gtacgcctgg tcgtacgagt acaccgagga tttcgtcctc ttcgactacc
    16021 gcatcaagaa catcggccgc gagacgctgc gccaggtgta catgggcatc tacgtcgacg
    16081 acatgatctg ctacgactgc ttcggcagca acctggggta caccgacgac cacagcggat
    16141 ttctccacac ctacccggtg cggtacggcc gctgcgagta tctcgacacc gtcttcctcg
    16201 cctggcaggc ggacaacgac ggcgacctgg agacgatatt ctcggatggc cggcggcatc
    16261 cctgcccgga cgtgatcgcg acccgcatca ttcggacgcc ggcggaggat ctcgatgtga
    16321 gtttcaactg gtggatcggg aacagcaacg cgcagcttga tttcggtccg cgcgagcggg
    16381 agttcgtggg gcgctggaag gagccggtgc gggatttcgg cacgggcgga ctggggacgc
    16441 ccgaggggga ccggaacaag tactacgtgc tgcgcaacca ggagttcgac tacgaccaga
    16501 tcttcacggc gaccatttca agctccgaca gccgctggct ctacccggac ccgagcctgg
    16561 cggccgatta cgccgatggc tatgacaccc gctacctgct ctcgttcggg ccgtttgata
    16621 tccagcccgg ccgctcgctc ccggtctcgt tcgcctacat ggccggcgaa cacctccacc
    16681 gctcccccga caacgccgcc catctccccg cgcggcccga cctctactac gccaaccttg
    16741 atttctccga tctcgccctc aacgccggct gggcctcccg cgtctacgac aaccccggcg
    16801 tggacaccga cggcgacggg taccgcggga gatttcgcct gatgaaaacg cgggccgacg
    16861 gcaccgtgga gtgcctgccc gacgggatct acatcgggcc gccgggcgac agtctgcagg
    16921 ggggcgagcc gaggctggat acggtgtggt atcagggcga cggggtgccg gatttccggg
    16981 gggccgcccc ccctccgccc cccgaattcc gcctcgcctc tccctcggcc ggcacgatcc
    17041 tggtccgctt caacggcctg ctgtccgaga ccgcccagga tcaattctcc gggctgtacg
    17101 atttcgaggg ataccgggtg tacctggggc gcgatgaccg ggaggccagt ttcagcctcg
    17161 cagcctcgta cgaccgtgag gacttcaaca agctcgtctg ggatgccgac gcgtccgagt
    17221 ggcggctgtt cgatcccccc tacacgctgg ccgagctgcg ctgtctgtac gccgattcgt
    17281 gcggcgatgc gacgtttgac ccgttgcgct acacccgggt caaccgcttc gtccacccgc
    17341 gctacccgga ctccctcttc tgcttcgaga agcaggatta caatgccgcc gggtcgggcg
    17401 atctcggcgc catcgtcaag agctatcccg accagccgta tccctcaagc cccgatcccg
    17461 cgctggtccg gccggacgaa ctgacggccg aggggcgact gaaatacttc gaatacgaat
    17521 tcaccatcac cggcctgctc ccctccgtgc cctactgggt gaacgtgacc gcgttcgatt
    17581 tcggttcgcc gagcacgggg ctgcccgccc tggagtcctc ggtcacggtc ggcgcgcagt
    17641 cgatctacac ggtgctcggc ggaacggagg cgggcgagag gctgcgggtg atcgcctatc
    17701 cgaatcccta ccgggccgac ggcgggtacc gggggaacgg gttcgagggc cggctggccc
    17761 cggaccggcc ggaggaccgg gtgcgggccg tgcatttcgc caatctcccc gcccgctgca
    17821 cgatcaggat cttgacgctc gacggcgacc ttgtccggga aatcgtccac gatcagccgc
    17881 cgggatcggc gcgcgccaat cacgaccagt gggatctcat cacccgcaac acgcagacgg
    17941 tggtcagcgg gctgtactac tggacggtgg aagacgggca gggcaacgtg cagctcggca
    18001 agctggcgat catcatgtaa tgccgggcgg cccgcgtcgg accaccgcca cgcccggtca
    18061 cgggcacacg ggccaggggg cgccgcgaaa catcgccgcc accagcaggg tgaggtcgac
    18121 gacagtgagt tgcgcgtcgc ggttgaaatc gacctcctca aaacacaacg gggcgggacc
    18181 gccgcggaag agatgggcga cgaaagcggt caggtcggcg atagtgacgg cgccgttgtg
    18241 ctggaaatcg cccatgtgcg cgcagcagcc gccttcgcag ccgaggccgc gggcgccgat
    18301 gaccgccccg caggcgttgt tctccggcag gcagacggag ccggccgcca cccggtagtc
    18361 gccggcggcc gtgtcgcaga agagcgggtc gaccgagttg ttgttggcgc tgtcgatggg
    18421 gtcgtcggtg aagatcacca cgtccgccgc gccgttgccg aaccagtcgt tgcaggtgac
    18481 aaagtacggg cagggcgcgg agccgtgcag accgtcgccg tggttgaagg cgaagacgtt
    18541 gccgattatc cgcgacaggc ccgagtccag cacggccggg gccggagcgc cgtccttcgg
    18601 aaaatcccag tcgtaccaga gcccggtccc attatccgcg aaagtgcagt tggtgacata
    18661 gagaccgcgc ggcaccattt cgtaccaggc gaaaattccg cgctgggcgt tgccgacaaa
    18721 aagcgagcga tcggcccgga gcaccgtccc ccagccaacc tgtatgccgt ccgcgctgtt
    18781 gtccaagaaa ctcgtcgagt cgaccgctat cacgccgctc gtcccgccgc cgatcccctc
    18841 ccaggagttg cggctgatgt cgcagccgat cacccgcaaa gtgtctccca gctcggctga
    18901 gaacatgatg cccgagcccg cgttgtcgac aatgcggcag ttgcggaaag tcggccccgt
    18961 gccttcgcac ttgatgacgc ccgattggga ataggggcgg atgaagccga gaccgttggt
    19021 gatggtgaac ccgaccacct ccgtcgtcgg cccctcgtcg ttgcggaaag tgaacgcgtg
    19081 gcgctggagg ccggcgtcgc ccccgagatt aaggattgtg gagtccgggc cggacggggc
    19141 atagaggacg acctgtttgc cgaggaagtt gaggtcgcgg ttgaacgggc cggtgtacgt
    19201 gccggcggcc acggcgatcg tgtcgccgtc ggcggcggcg aaaatggctt cctgaatcgt
    19261 gggaaggtcg gccggcacgg cgattttccg atggggggcg atctgcaccg cgaaaagccc
    19321 ttcccgccag gaaccccagc cctcggcgtt ctgcaggcgg aggcggtagt agtacaaccc
    19381 ccggtcggcc agcggcccgc ccccgtacgc ggctgccggg atgccggacg gtaccggccc
    19441 ggatgcccac agctcggccg ccgtccagtc gcggtcgcgg ccgatctcga tttcgtaccc
    19501 ggtctgcgtc agtccgctcg tatctgcgta ctgccaggcg atggtcggaa cgagagtccc
    19561 ggagtcgacg gcgatctgcc aggcggcggg cggcgcaccg gccaccgaga aagcgccgag
    19621 atcggtcggc gtgccgtccg gatcccggta atccggatgc ccggcgtcaa tcagcggcga
    19681 accgatccac aggcgcaggt cgcccgcagc cgaatcgcac cacagcgggt ccgcgagcag
    19741 atcgtgttcg gcgtacggga gacagcagta tatgtcgtag aggacgaggt tgtagtcgat
    19801 ccgggcgagc gaggtgatga gcgcccggtg ctggcagtcg atgatgatgt tgttgaggat
    19861 ggcggcgctg tcgtgcgagg ccgatatccc tatataacgg gagtcgtaaa tggtgttgtt
    19921 gtagatccag atcggccccg ccgccttgat ctgataataa ccgtcgttgc gaagaaacaa
    19981 gttgtgatgg atcgcggggg cgctcgccga atcggccgag agaaccgccg gcgtgttgct
    20041 gagattcggg cagcgggtga tccggttgaa acagatcgtg ggggaactcc cctcgatcac
    20101 cagggcgctc gacatcccgc tctcttcgaa actgaacccc cgcaccacgg cggcgcgggt
    20161 ctctccctgg tggatgcgga caagcgggag gccgccgtcg gcgggtttca ggatcgtgga
    20221 atccatgccc gctccgagaa ggacgatgtc gatcccggcg aggctgatgt tttccagata
    20281 ggttccgggc gccacctgca cggtgtcgcc ggccgcggcg gcggcgatgc ccgcctggat
    20341 ggttgcgtgg tcgccgggaa catggattac ggacgcggcc gcggcacccg cgaggagcgc
    20401 cgggaacaga ccgcaaacaa ttgagtattt catggcagcc ttctttggga tgagcgcggt
    20461 accgatgaac gcgttcctgc tttctcccaa gatagcgcgg atcgggggtt ctgtcaatac
    20521 acgtgtggtt tgacattgtc ggcggcgggg cccatattgt cacccatgtt tgtcgactac
    20581 gccgagatcg aggtcaccgc cggcaaaggc ggacacggag tagtctcctt ccaccgggag
    20641 aagttcatcc cgaaaggggg tccggacggc ggggacggcg gccacggcgg gagtgtcgcc
    20701 gcggtggccg acccccaact gaccaccctg ctcgattacc ggtacaagaa gcactaccgg
    20761 gcggagaacg gacagcccgg ccaggggtcg ctcaagaccg ggcgttcggg gcacgatatc
    20821 gaattgcggc tcccggtggg gacgatcgtc accgacctcg acaccggcga gcagcttgcc
    20881 gatctcgact cgccgggggc gagagtgatc ctcgcccgcg ggggcaaagg gggactcggc
    20941 aacaatcact tcaagacctc gaccaatcag acgccgcgca aggcgacgac ggggtatccc
    21001 ggcgagcacc gccggctggc gctggagttg aagttgctgg ccgacgtcgg cctggtgggc
    21061 aagccgaacg cggggaaatc gactatcctg gccacgttct cggccgcccg ccccaagatc
    21121 gccgactacc cgttcacgac gctgatcccg aatctgggga tcgtccgact gcgcgagcac
    21181 aagtcctgcg ttatggccga catccccgga ctcatcgagg gggcggccga ggggaaagga
    21241 ctggggctgc agtttctccg gcacatccag cggaccgtcg tgctcgtgta tgtcgtcgat
    21301 atcaacgacg gggatatcga agggacgctc cggctgctgc ggcgcgagct ggcccggtac
    21361 gacgagtcgt tggctcggcg gccctcgctg gtggcggtga cgaagatcga cacggtgagc
    21421 gaaagtgatc tgaaagcggt ttccgaaaag ttgtccgacg gctatattta cctgtccgcg
    21481 gtggccggcc tgggggccgg gagcttcctt gaggcgatag agcgaatcct tgaccagcag
    21541 cgagcgacat agacgattag tcgacacgat cgggctgctg caggtgcccg gcatcggcgg
    21601 cggccgattc gaccgacttg tccgcgcctt cggttcggcc ggcgcggcgt ccgcggcctc
    21661 gatcgccgag ctggcggctg tcccgggcat ttcccgcgcc ctggcgactg ctatccatga
    21721 gcagtatgac gaggcgtcgg cggaagcgac cgccggccag attgcgcagc tgggctgggc
    21781 cgtgattttt cccgacgatg cggactaccc gccgatgttg agcgcactcg agccgccacc
    21841 ccctcttttg ttccgggtcg gagagccggc gcacgcctcg gatcgcatga tcgcgatcgt
    21901 gggcacgcgc cacccgaccg aacgcgggcg gatgttcgcg cacggcctgg cgcgggcgct
    21961 ggccgagtcg ggcatcgcgg tcgtgtcggg catggccgag gggatcgaca ccgccgccca
    22021 cacgggagcg ctcgacgggg gcggccgcac ggtcgccgtc tggggcagct cgctcgatca
    22081 agtttacccc ccgaccaacc ggggcctggc cgaccggatc agggaacggg gggcgatcta
    22141 ctcggagtac ctccccggca cgttccccga ccgcccgcat ttccccgagc gcaaccgcat
    22201 catctccggc atggcctggg gagtggtggt ggtcgaggcg ggagaccggt cgggggcgct
    22261 gattacggcg gaacacgccc tgcggcagaa ccgcacggtg ttcgccgtcc ccggcccccc
    22321 gggtgcgacc gccagcctcg gctgcaatga acttctcaag cagggggccg ggctggtgac
    22381 ctcggtcgag gacatttttc gcgagttgcc caccctgaga ggcgaggtca aggtgcagcg
    22441 ggcggccgag cgcccggacc tgacgccgac cgagcgggaa atcgtggccc tcttcacggc
    22501 cggaccggtg cagctcgatg acctcgcgcg ccgcctgaac aaaccggtcg ccgaactcac
    22561 ccagttcctg ctggcgctgg agctcaaggg ggtcgtcctc gaactggccg gcaaacgctt
    22621 cacgctgagc gacgaattcg catgatcacg gccaccgcga cgatcgtcaa caaactgggt
    22681 ctccacgccc gtccctcggc catgctggtc accaccgcat cgcgcttcga gtccgaggtc
    22741 tgggtcacca aagacggttt gcgggtgaac gggaaatcga tcatgggggt aatgatgctg
    22801 gcggccgagc gcggctcaca actgctcatc gaggtgaacg gacccgacga gcaggaggcc
    22861 ctccaggcag tcctccgcgt gatcgagggg gggttcgggg aacggcccga ctgatattcc
    22921 gcttgccttt agtctgcggg gcttcctata atgtatggtt accccggaaa ggggtatgat
    22981 cggcatggat ggcaaggaat acaccataga cggggtgccg atctccagcg ggattgttct
    23041 cggaaccgcc cgggtgatgc tgccgggcga ggcgcaggta tcggaaatgc cgatcccggc
    23101 gtcccgggtg ccccgggaga tcgacgccct gcagaaggcg gtcgacgaga ccctcgacga
    23161 gcttcgcgag gtccaccagg cggccatccg caagattggc ggtccggtgg cgaagatatt
    23221 cgaagctcag gtcatgatcg ccagcgacac cgagttcctc aagcgggtgc gggccgccat
    23281 cgaggagcag cggctcggcg ccgcctatgt ctaccagaag ctggtgcagg agacggtcga
    23341 cccgctgcaa aactcccccg accgttacat gcggcaggta gcgctcgata tcgaggcggt
    23401 tgcggcccgg gtggtggccc ggctatcggg gcggatcccc cagcagaagc ggttcgcccc
    23461 caacaccatc atggtgggga aatcgttttc ccccaacgac gtggtctcgt accgggagcg
    23521 caaagccacc ggcttcgtca tcagcgaagg gggcgccgat tctcacatgg cgctcatcgc
    23581 ccgcgctctt ttgctgccgg tcgtgctggc cgaggaggca tggcagaaaa tccccaacaa
    23641 cacccggatt atcgccgatg ccgtgtccgg ccgggtcatc gtcaacccca gccaggcgac
    23701 ttgggaagat taccagcggc gccggcgacg gctcgggccg accgtgctca gccgcatcaa
    23761 gaagctcggc cgcatcccgc cggtcacggc cgatggcgtt gaggtggcga tcggggccaa
    23821 cctgacgctg cccggcccgg ccgatgaaat cctcgcccac cagcgcgtcc aggtcggtct
    23881 ctaccgcacc gagttcttgt acctcgcgaa cggggcgttt ccctcggagg aggcccagta
    23941 cgattactac cggcggatcg cccggacttt cggcccgacg ccggtggtgc tgcggacttt
    24001 cgacctcggg ggcgacaagg tgttcaacgg cggcggcttc ccggtcgagg acaaccccgc
    24061 cttgggactg cggggaatcc gcactatgct ggcgatgcgg gaagaattcc gcacccagat
    24121 ccgggcgatc ctccgcgcgt ccgcccacgg caatctgcgg atcctgctgc cgatggtcac
    24181 cgagctggag gaaattgagc gggcccggaa gatcatttcg caggagaagc agaaacttcg
    24241 gcgggaagct atcccctttg gcgaagacgt tcccctgggg atcatgatcg aagtcccatc
    24301 ggccgccctc acggccgaca cgttgagtcg gcgtgtggac ttcgtgtcga tcgggacgaa
    24361 cgatctgacg cagtatacgc tggccgcgga ccggatgaac agccacgtgg cgcacctgta
    24421 caacgccctg cacccggccg tgctgaacct ggtctggcat acggtcaaga cctgccgggc
    24481 caacggcgtg ccggtgaccg tgtgcggcga gctggccggc cagcggctgg ccctgcccct
    24541 gctgatcggt atgggcgtgg acgtgctgtc gatggcgccg gcgcgcctgg tcgaggtctg
    24601 ccgtttaatc cgcaaaatca actccacccg ggcgcgggac ctggtcgggc cggtgctcgc
    24661 cggcggcacg ctgcaggcgg tggtggaccg cctgcgtgac tatagagaga ccctggaaca
    24721 cgtggacccc agaatgagag aggacagctg attttgaata tactcgacga cattgagaag
    24781 atccgcgccc acgatccgga aaacatgtac aaccgaatct tcgattttcc cgagcagttg
    24841 gcggatgcgg tgaaactcgg caaactctgg aagttcgatg cgagcgcgtt cccgggcatc
    24901 aagaatatcg ttaccgtggg catgggcggg tccggaatcg ccggcgacct catgcgcagc
    24961 tacctccgca ccaaactgct cgtgccggtc gacatctgcc gctcctacgc gctgcccgag
    25021 tatgtcgacg acgagaccct ggtggtcgcc gcctcctaca gcggcaacac cgaggagacg
    25081 ctcgcggcgg tggacgacgc gctcaaccgc aaggccatgc tcgccgcgat cgccaccggc
    25141 ggtctgcttg aggaagtggc ccagctcaac gacatcccgc tggccaaact gcccggcggt
    25201 ctccagcccc gcgccgccct cggctacctc ttcgtgatga ccatgatgtt tctcgaacag
    25261 gtcggccacg ccaaggggtt ctccaaggag ctcgagaagg tcatcccgca gctgcagaag
    25321 caccgggaga agtatatcga agacaattcg acgaccatca acccggccaa gaacctcgcc
    25381 cagaaactgc acaagaagat cgtgatcgtc tatagcggac cgaccctgac cgatgcggtc
    25441 gggcggcgct ggaaagctca gttgtgcgag aacgccaaga ccctcgcctt cgccaaccag
    25501 ttcccggaat tcaatcacaa cgagctggtc ggctggtcgc cgtcgatcga ggaactcgcc
    25561 tcgcacctgg ccgtcgtgat cctgcgcgac cttggcgacc acccgcaggt gcgccagcgc
    25621 atgaacatcg tcggcgaact gatccgctcg cgcggggtgg aggcgatcga cgttcactcg
    25681 cgcggggaga ccgagctcga gcggatgctc tcgctggtcc agttcggcga cttcgtctcc
    25741 tattacctcg cgattctcaa cgaggtcgac ccgacgccgg tgaagatcat cgacgccctg
    25801 aagaaggcgc tcgcggaggc caaaaaatag cgccggggga ccgacggcaa ttgggcgggg
    25861 ccgccagacc ggccccgcct ttttttctcc cgcgcgcggc ccggcgcgcc aaaccctccc
    25921 ccgcgtcttc cacggcactc tgtcgggctc cgggcgttta acagtttgag gcggcgcgcc
    25981 gcacggcgcg accgcgatcg gtcccgagcg gagcaggaag atgattacgc cggaaaccag
    26041 agtcgacgaa atggtggagc gctatccgga cgcggtgcgc tttctcatga gccggggact
    26101 gccgtgcgtc gtgtgcgggg agccgttctg gggaagcctg gaggagttgt gccggcagaa
    26161 gggttggccg acggtccgcc ttgcggaact cgtggacgag ttcaatcggc tgcactcgtg
    26221 aagggggagc ggccggtgcg gcggtcgcag agactgtgtt gcggggcccg gccggcgaac
    26281 agacgacggg cggggcggcc acgccggcgc gcgcctttcg catggtacgt catcctaccg
    26341 gagacgacag gaaagcagcg cggaatctgc tccgcggcgg gtgagttcaa aaatcccatt
    26401 gacaaccggt gcgctccaca ttatagtaga aggggcaggg acttgaccgg aggtatgctc
    26461 atgtctctgc acaatcccct ctctcgcccg ccggccctgt gtctttggca aaacttgtgt
    26521 cactataaac caaggagtgt gtcatgaaac gtcttctcac tgtttcggtt gtcctggtgc
    26581 tgttgttggc caccgccgtc agcagtgtca gtgctcgccg gaacccgatt gctgtcccgt
    26641 atccgcggcc gtccagcgag gaccatccgt ggggcgggga gcagaactat ttccctgatt
    26701 ctcgcctgtc ccagcccggc acttcgtcgg gagtggcgcc cggaattgtc ggcattgaga
    26761 ccagcctgtt gtggcggctg gtgatgccgc tctggttcga acggctgacc ggccacacca
    26821 tcgtgacact gccgcagccg tcgactgacg gcaacgcaat tgtgggagag aacaacccgg
    26881 gtgcgacggc cccggcaaac aacggtcgag gaaactgagg gaacgaaggt atgagagtga
    26941 ttaccgacac tgccgacgga ctgcttgcgg cagcggctca ctacctggag gaattgctcg
    27001 ccgcgcggga tttttcgggc gcggtcaagt actacgaggc gaatcgccgc cagcttgaga
    27061 cggcgggaag cgcgctcgcg ggacgaattc tccggttggt ggcccgggct caccttgcgc
    27121 tcgcttcgta ccccacggcg ctccgcctgg cccgcatggc gcaggccatc cactccgagc
    27181 acggcgacac gctcgagttg gcggagactt tcctcacggt ggccgaagtt cttcgggaca
    27241 gcggcgaact gaagcaagcg gagaaagcat tccgagacgc cgaatcaatc tttcggcgca
    27301 acgattgtgc ggaagggcag agcagggcgc tgaacctgct ggccggcctc tacttccgcc
    27361 aaaacgacta tcgcaattcg ctgacggtgc tgatggaggc catcggcctc gccaagcgcc
    27421 tggacgaccg caagaaactt gcgtacatga tgggcaacat cggccgtatc cagacattcc
    27481 tcggcgaatt cgcagaggcc aagaaacatc tcgaaatcaa cctcaccctg tccgaggaac
    27541 tgggacagac tctcgaggcg gcgcgggcgg cgctgtccct cggctacgtg cagatgcagg
    27601 aaggggactt tgccggggcc gagacgacgt tccagaaagc ggcggccggt ttgcgccggg
    27661 tcgaaagccc gcgcgatcag gtaatcctcc gcacctacct gggcgagctg tactaccgga
    27721 gcgagcgggc ggcggaagcg gccgagatgc tcgagagcgc gctggcggcc gccgaggcta
    27781 tcggtcggga gacgaccctg accggccgcg tgctgcgcca cctggccgag gcctcgctgc
    27841 gtgtcggcca cgaccgggtt gccgcgcgct atgcggcccg ggcgctggcg attctggcca
    27901 aaaccggcga gatggtcgag cagggagccg tgctgaaagt gcaggccatc ctcgccgaac
    27961 gcggcggtcg catcgccgaa gcccgcgagc tgttcgtgaa agcgctcgac acgctccacc
    28021 agtcgggcgt tcggtttgag aaggccgagg cgctggtggc ggccggccag tcggccgctt
    28081 tcgacccgcg caaacgcctg acctgcctgt tccgcgccga agagtatttc cgccgcgggg
    28141 ggatgcagcg gcgtctggag gagattgacc gcctgctggc cggcacggac cgcctgcggc
    28201 cgggcgacgc ccccggcgcg gccgcgagga cctccgaggt ggaattcctg accgccaacg
    28261 agcagatcaa gcggttcaaa cagcaactga gctacgtcgc caagtccgat ctccccgtcc
    28321 tgctgaccgg cgagacgggt gtcggcaagg accacatggc ccggtacttc cacgcgatct
    28381 gccggcccga tggtcctttt gtggcggtca attgcgcctc gattcccgag acccttctgg
    28441 agtcggagct gttcggatac gccaaggggg ctttcaccgg agccgaccgc gacaaagaag
    28501 gacttttcgt gaccgccaac ggcggcgtgc tctacctcga cgagatcggg gacatgccgc
    28561 cgactctcca ggccaaactg ctcggcgttc tggaaagccg ccgggtggtg ccggtcggat
    28621 cgacgaaaga ggtgccgctc gacatccggc tcgtcgtggc gaccaaccgc gatctccagg
    28681 cgatggtgga gcgcggattg ttccgccgcg atctctacta ccgcatcagc ggcattacgt
    28741 tccacctccc cccgctgcgc gaccggaaag aggatatccc gctcctgctg cggcacttcc
    28801 tccaaaactg ccgccgcctg gcggagagcc cgctgccggc cgacgttatc caccgctttg
    28861 tcgagtatgc ctggccgggc aacgtgcgcg agctgaacaa caaagtcaaa cggctcgagg
    28921 tgatggcgga actggccgcc gagggagatc tctccgaatt ggcccatgcc gtgttcggcg
    28981 accccggtcg ggatcaacgc tcctcgctct tcgagcaggt tgagcggttc gagcggcaga
    29041 tgctgatcga ggccctgctg gccaccggcg gcaacaagtc ggaggcggcg cgcatcctcg
    29101 gaatccacga ggccaccgtc cgcacgaaac tcaagcgcta cggcatcagc ctggaaggag
    29161 gtgcgctcaa ctaggctgaa accgacacga gccggatttc ctcgccccgc tccgtgtgat
    29221 cgtcgcgacc gagcgtgtga gatggcgctt ggaggcatcg gtaccggaac cgggcgggtg
    29281 gaatcgtagc ggcttacgaa cagacacagt tactccttgg ttgcggtaag cgtttgattg
    29341 acaggcgctt accgcttttt ttcgggaccg gactcgtatg atcgtacgaa attccacgaa
    29401 cgaatgtgga acagggaacc gtagcacaga gctaatatgc tgtgatgcaa acgactacgg
    29461 cgtcgtcagg caatgcgaca ccatgcggca cgaagcttgc tgttccaata actgtcatct
    29521 taactccctg agcgttgcag taattgttac gcttatcttt tctctttggc ggatcgccac
    29581 ccgtgcttga ggggagcacg ggtggtattt ttttggccga tcactttaag aacgaaccgc
    29641 cggagagcgg gcgcttcagc tctgggcgga tatcgccgct tgtgcgcgta atgggattgc
    29701 gttttttcgg gggcggcgtt atttggatgt gggaagggaa ggccgatgat ttcaacggga
    29761 ccgccaggaa cggaggtatg cagtgagtac atttgaaatg gtcagacagc ggatccggtg
    29821 gtatcacgtg gccgggctcg cggtgctggt ggtggtgacc gcggggctgg tctggttctg
    29881 gttggagcgc cgccggaccg cagcgctccg ggctgaagat aagacggatc tggccggcgg
    29941 ggcggagtga gaacacttcc cgggggcatc cgcgggtccg cggtatctcc cgggctgaac
    30001 ggccggcttg cgcgcctgcc ggaggcgacg gagactctcc gccgggtgaa ccgcgctcgt
    30061 cgggtcgatc tgtagccatg gcattactca cggacaaagt ggcggtcgtc accggggcct
    30121 cccgaggggt cggggcggcg accgcccggc ttctggcagc cgaaggggcc cgggtcgtgc
    30181 tctgcgcgag gcggcgggag gggctcgagc aggccgccca cacgctcgat ccggacggct
    30241 cgcgctgtct catcgccccg gcggacgtcg ggacgcttga cggtatgaag ggactggtcg
    30301 acgcggcgct cggccggttc gggcgaatcg acctgttcat caacaatgcc ggggcgggag
    30361 tcc
//
LOCUS       DAOPOE010000031        30226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_1966_length_30226_cov_5.951841, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000031 DAOPOE010000000
VERSION     DAOPOE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30226
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_30226_cov_5.951841"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2565)
                     /locus_tag="PKM94_05885"
     CDS             complement(<1..2565)
                     /locus_tag="PKM94_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD66207.1"
                     /translation="MPNHPRLDDVTTAGPAHSAFKKPAEALEQHLLPPGLQVPALKLD
                     YFCDVQDYTSGSPAYFWTIPDAYGDDLFNMRFTADAGYDCTLKVAWLLMYADAQVGSP
                     TLRVYLWDDDGFGFPGNKLDSVDIATPPAGGSGLYWLSADFSAADWVFSDGVNYHLGF
                     TQLDQAGSVVAVISDAADGPFAGEERASEYYGTSWGTMLGDWGLDVSFFIQAERCCAE
                     IPFTDCYSQAYIENVAYFWRAPHPVYGDEQYAEKFDVGGPETLTSIDIAIYGGGATWG
                     SVTDPVGNNDIYIRVYDDNAGFPGDLLTTVTLPAGTYALYPALQNVPMPANFVLTNDF
                     HVSFGTNGVFATSWESCLSSDGTDGSGRSSSYFDGGTGYEWFSFLDGWGMDAQFYWVA
                     NLCKDEYSDCSINYCYNGTAYYWQLPDRYGDYGEAQKFSSVGEECRVDAVTIAWYWNR
                     AQYSWPRYTTPSYIKVWADAGGAPGAELGSLTVNPADYGIATPTPTASVTHWATYDMG
                     GITVVGDYWVGVISDAADTLTGISVLSDAGGGGCMEGYAEYWGDWGLMYVDWGFGAGY
                     EDLAMVIESEHCCIPYSERTCTTPTDWFTRGYGYNRSNASFVQMSDAWCDLTLNWAYP
                     AATPVGFAGPVVYDGKVVCSFLNNYKVFDLISGALLTTITPQAGPFAPGDIRCTPTVT
                     TIAGYPDPVLFVTGGTNDEVLAYNFNTYALLWSRHIGNMGPTGVFGDTRFGVFVVLNQ
                     GGTDVVYWTTDGGRLVAADALTGALYGGWAVNPFNPGKPCHSSLATNGSLLFFGTQAS
                     GVEGDVYAIDAATGAMVWQLSAAGGLQAATVYASTGGYAGTEGFTGGIAFDPQRNLL"
     gene            3441..5885
                     /locus_tag="PKM94_05890"
     CDS             3441..5885
                     /locus_tag="PKM94_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOD66208.1"
                     /translation="MTPPAVFFLAMRLSSLKIRNFRVLKSVDLTFADEVIGITGGNGA
                     GKSSLVEAIAWALYGNQAARSRREEIKAAFARPEDLCEVSLEFLINDEKYRVIRRLVG
                     KSERPEVELYRGESSESVGVNETRKYVGELLGLDWRGFLTSFLARQQELNALSVLQPA
                     RRKDHLAGMLGIDRLERAIVRVKEDVKIAGGQTELLARQVADGESLTTAIAQLTARRS
                     ELEGLQSAAAAERKAAEEAFAAARAEYIKAQETRAAWSELGAGLEAARKTAAALAVRL
                     KELEADTVQLAQADSAAAEVRARIAALEPARRELEVLSEARARRQLLDQMERQRTELQ
                     AELDQTARAVEESARALAGLESDLAAIPASITAAAEAGKADLDAARQEYGRLLAEQKS
                     IEVQKEKLAAQMRSIASLGPDSVCDRCLRPFGDDLPAIRRHLAEELEQLTAAFAGLGR
                     ELASAAARGTQLKQQAEALEARSLARRELVIRREAAAREREALSARLAELGRRGEAAR
                     VKIAEIGEVVFDEDRFAQAQQAVAALEAEHRQLHRLEGQLARREAVTAARARTDAALV
                     QAQAEADTLLQRRDGLGYAEETFAACRERFEAAQARFETAREAAVGAVKEFELTGREL
                     DGKREQLARLEAARAQLDQSRARQYYGEKLAGLFAEFRELLISRIRPTLSDISGRLLA
                     EMTDGRYSMVELDEKYNLQVLDDGQFYGIDRFSGGEKDLANLCLRLAISEALLQSAGI
                     DRSFIILDEVFGSQDDGRKELIVQALTNLTHRFPQIFLITHVGDIRDRVECLIEVAPT
                     GLGWSKVTVRGEETEA"
     gene            5863..6525
                     /locus_tag="PKM94_05895"
     CDS             5863..6525
                     /locus_tag="PKM94_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOD66209.1"
                     /translation="MARKRKHKARLAGRLALLLVLIAAVLIVRRSDIGRDPAPVPLTT
                     VVKIIDGDTMVLGNGETVRLLAIDTPEDGEPLHDEAAALLAQVALGKEARIVSSGRRR
                     DTYGRLLAYVYVDDTLLVNRVLVDSGLAYVYLFRDTDTGRPETDRIIAAQRAALDRRA
                     GLFALPRAAEPYYVAGRTSYRFHRPGCPSTAEFTSGRARTFATRRDALAEGLSPCRRC
                     KP"
     gene            6704..8668
                     /locus_tag="PKM94_05900"
     CDS             6704..8668
                     /locus_tag="PKM94_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66210.1"
                     /translation="MSFRTILPVLSAVAVLLAAATMADAPRVISFQGRLLDDAGAPLP
                     DGPKSIRIIIWNDPASTLPSHEVWNSGPLTVETAGGLFSLNLGETPMPPLGPGLLADS
                     GLWIGITVGAEAEGSPRIKMTSSAFAFRATLADTAAYADEASYAGEAAHADEANRAGA
                     AVQADSAGYADTAAYAFSAPVSAVDHGSLAGLADDDHPQYLRADGGRMMTGDLDLGKH
                     QIHSLEAGTTSSDAVRYDQAVKDGDAAGGDLTGTYPAPQLAADAVGTAEIAAGAVGTS
                     EVADNSLTATDLASSSVSTAEVTNNSLLHDDLATGAVRSDEIQDGAIVNADINAAAAI
                     APSKIAGTACDLATDQAITGNKTFRAATYFYDSTMAVGSYSVLIGTDSITPQWTYPLQ
                     VSRKYSTSNVTHGIDVHLRNEGIGNIWGYYSRVGVNDASSGTRSGIQVTAGDSSNTVG
                     TSYGVRTYAYGGDQTYGIYARANAGDIVNYAGLFSGNVDVIGTLSKDAGAFRIDHPLD
                     PDNKYLQHSFVESPDMMNVYNGNVTTDAQGDAAVALPDYFESLNKDFRYQLTVIGQFA
                     QAMVASEISGGRFTIRTDKPNVKVSWQVTGIRKDPYAEAHRIQVEVEKPPYERGSYRF
                     PEGFGLGSERSVDRANDAELENSAPAPAAQ"
     gene            complement(8744..9250)
                     /locus_tag="PKM94_05905"
     CDS             complement(8744..9250)
                     /locus_tag="PKM94_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66211.1"
                     /translation="MKNALCGSLAILLLAALAVPGALAQESYNQPGFRGWGPRLGATV
                     DPDQVHFGLHVDVGNFAEHIRFEPNFELGFGDNVTLAAINFDALYRFASNWDVWSPYL
                     GGGLGVNIYSFDIEGVGNHSETELGVNLIGGIERGLASGDRFFIVGKIGLADAPDFKV
                     TVGWTFLN"
     gene            complement(9310..9840)
                     /locus_tag="PKM94_05910"
     CDS             complement(9310..9840)
                     /locus_tag="PKM94_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66212.1"
                     /translation="MTARLIFLCFGPALMLLAPAGAAAPTGPSGTTALGWGPHVGLTV
                     DPDQIHLGVHLQVGPPQPGVRFRPAFDLGFGDNVTAIALNFDAVCLLRTRDPRLMPYL
                     GAGIGANYYDFDRGVSARRRDDRDEGDGSDVEPGVNLLGGLDWRLRGGAHFFGEARIG
                     LVDAPDLKLTIGWTFF"
     gene            10139..11512
                     /locus_tag="PKM94_05915"
     CDS             10139..11512
                     /locus_tag="PKM94_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD66213.1"
                     /translation="MPNILVVDDKDSMRNMLAETLSEEGHRVDAALDGRSAIDLVRNK
                     SYDLVLTDLKMPDISGLEVLTTVKEVDSETAVIVMTAYGTIEDAVAAMKAGAYDFITK
                     PFDTEHLCVLINRALENRRLMAENTLLREEAMSGKEFANIIGKNHQMAEVCALVQKVA
                     GSDASVLLQGESGTGKELFARAIHGLSSRANGPYITINCAAIPRELLENELFGSEKGA
                     FTGAHARKMGKFEIANTGTIFLDEIGDMDIALQAKLLRVLQQRNFERLGGTKPIEVDV
                     RVIAATNMDLTELIRAKRFREDLYYRLSVFPLTIPPLRERLDDLPLLADFFIAKYCTE
                     MRKPVKSLTRPALELLQKYHWPGNVRELENTIERAVILAEGKKITPDHLAIRLRRTSE
                     IQLRDGAGLKEIGALAQMQAERSVILRVLKDTRGNKRKAARILKIDYTTLFDKIKKYD
                     IESALNE"
     gene            12059..13780
                     /locus_tag="PKM94_05920"
     CDS             12059..13780
                     /locus_tag="PKM94_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66214.1"
                     /translation="MFTTGRIRRLAVLAPVAALALIFACSDSGTDGPPVEGDCTTLTA
                     QANAALAALMQGDIDSIINHGDSTFRPHDVDFSIPHGLYQQALAADPNCADAKMGAAF
                     TGVLMFMTDADFNSLVEKFKHAYDTLTFNPMRPNSLAPAVGFGGDILTDAIPLKAGRF
                     TGLIPSPLELDRAALRLAAVDPDLTALQDRLEESLLPKIRSARTRLLDLVADPDYTFE
                     ITPAMQGNEGAEPVLLDRSDIQLFIAMTYAAEAGLEVFFARNLSLSAYTTAGVEEALA
                     RTSEFLSLKSGGVGTQHMGNAKQCVLDAVNALKTAIGYLRAEIDTYQPYDFIEVYEED
                     RDDLQDAENVADSVLDYFTGPRPLQVTFNREWECVWVVDHDSCYWVEDTLLLDVDIAE
                     FFDNPITNYKLFVPLYTVTVDPLNDLYKAHAAAHFSRARYWTALGALHGITAPNDAAY
                     FGYHLPDGDTDEFYRFLAEWPQSHQTVFGWDDVENWCGPGDTNLTCYFSLNAQNRYER
                     YWDSPDQILACYDWAAADFGSWTFPNPTMNGLLPGMTSDRLKEIWSVDLGFPWYPSQC
                     DTLGFDF"
     gene            13832..15256
                     /locus_tag="PKM94_05925"
     CDS             13832..15256
                     /locus_tag="PKM94_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66215.1"
                     /translation="MPKRSALAALLLSLLAGPPAAAVRLDGSSSGYQTMVLFRDSPGE
                     QWSVGRSRMEAAVSADRFGAGWQLRLGHTVPTGELRFGDYSGQWPAAAPGAADRFLGR
                     VGPPRPSTDVALAARFWRRRLAVELPCRFRSTGDPDCRLDVSFSPLESIRLGLAWGRV
                     TPDPGPIDLFYEYDDSGARARAGGRVHWWSPARLAQAEAEIAPLPHLRLRSVVRDLDF
                     APATPAFDGEPDGLYRACLEGSLHDGALGADWRLARGWLAAYTYRSLRLDARLQGYDG
                     GVPWAYFGIVRGDLHQHVLSLQRGDRRLSWEWGRGDGAVQGSVKLRPFLEGLYRLLGE
                     RRHIVASGAIDWKHLALESPLVSRGCVSLRGALDWLRAEPRVEWVTWRPVLGLGIDDL
                     RTGRWDLVRADLLRVRLLQTVRLGAVALDLDVSQWLPLSIRRAGGASAGSGAPGPADG
                     SVSESAGGGLWSGFSFAARVRLIR"
     gene            15515..16060
                     /locus_tag="PKM94_05930"
     CDS             15515..16060
                     /locus_tag="PKM94_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOD66216.1"
                     /translation="MQTSSLVNRRVLLLNQNYEPLSVCSAKRAIVLVIEGKAEMIESA
                     DGLKLRTVRTSYSLPSVVRLWNFRKVPLKRIMLTRKNIILRDDGRCQYCGANKGPMTV
                     DHVIPRTEGGSDSWSNLVCACERCNNLKGNRTPEQAGMGLLRRPTRPSYITFIQRNYG
                     VADQWRPYLFLDYNNGTTYTT"
     gene            16088..16582
                     /locus_tag="PKM94_05935"
     CDS             16088..16582
                     /locus_tag="PKM94_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66217.1"
                     /translation="MPDLLWLTDIHLDFLDTDGRRRFFRRIGESCADGVVVTGDIGEA
                     DTVEGHLAELAEAAAHLSEVLPGALARYSRLLVLTHVPPFREACRYAGRIADGDHLPH
                     FSSQVVGEVLEAHLRANPSRFATVLCGHTHGGGKAEVLPNLTVFTGAADYGRPRIQKV
                     LHLS"
     gene            16694..17569
                     /locus_tag="PKM94_05940"
     CDS             16694..17569
                     /locus_tag="PKM94_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HOD66218.1"
                     /translation="MTHPQGPPPYPTQAYTAGYGDAAGSCHTYAAPPPPGYAQPLSAR
                     GRAVATVYALGVTLLFHLISIPLLLWENSLVQRYGMAIWDGGIAEGEFGAWGLVTFGN
                     ALFLIAATVTTVVLFLMWIHRAYRVLEVNKVAGLKATAGWAVGWWFIPIANLVKPFEV
                     TKEIFVRSERPPAGGAPWGAGPTPAPAGFVVSTAQSRESSWIVGAWWLTWLAASFATN
                     LSGFLSGAFYGLGGNNQPTAEQYVRGNLLDIGATVLFILAGVLAIMVVRDIDRRQHRA
                     FSQQPAPLPPSAWPR"
     gene            17628..18380
                     /locus_tag="PKM94_05945"
     CDS             17628..18380
                     /locus_tag="PKM94_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66219.1"
                     /translation="MPESLIALLVFGGFAVVFVAVIRAARAQRRARIARLQQIGFFEW
                     KDPDQTLVARFVPLISGGGKKAPIGTVLRRTSGAGEMYTFLPHGDTSAAGVLIGRVPD
                     LALPRFRLMRRPPINGRLGTWVVGLVTKLMGGDLQPVAFASRPDLAEQFLAFAETPAA
                     FESFALRHWADLLKGAPVGFGLAGDRDLLAFSFHADLDRSARRRSALPALPSTVTMTR
                     DRQDLCARLAESVRQAEQFIAAAQTSWSAHTV"
     gene            18675..19979
                     /locus_tag="PKM94_05950"
     CDS             18675..19979
                     /locus_tag="PKM94_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOD66220.1"
                     /translation="MAKMRFSTMTTRLRRSEIRELLKLTRTPGTISFGGGLPDPVVFP
                     REAVEAASKRAIRERGDLALQYSPTEGEPFLKEQLAAYMQRHDEIARPEDMLVVSSSQ
                     QALDLLGRIFIDPGDPVLIERPTYVGSIQAWRALDADFQGVDMDDDGIIPEKLDAKIR
                     ELVDGGRPPKFIYVIPDFQNPSGINLSLERRHEVLAIASRYDLPIIEDSPYRELRFSG
                     ELIPSMYSLDTERRVILMKTLSKIYTPGFRLGWIVAPPDVLDKLIMVKQGADLCTSAY
                     VSIITGYLFEDGAIERQVERCRDLYARKADVMLAAMEREMPKIPGLWWSKPTGGMFLW
                     LRMPNHMDTMEMIHDAINLKVAYVVGTAFYTDGSGRNEMRLNYSYPTEEQIELGVERL
                     GKLIRMRVRGEKRAGKTPADDTDEEECAACADTGDRTSEQDR"
     gene            complement(20064..21167)
                     /locus_tag="PKM94_05955"
     CDS             complement(20064..21167)
                     /locus_tag="PKM94_05955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD66221.1"
                     /translation="MSSAPTTRHVVHFRPATALADLPEEGTALVVTSPPYPMIEMWDN
                     SFGDQDERIGDALRQEQGEAAFALMHQLLDRVWGEVARVLAPGGIACVNIGDAARSIG
                     SGFRLFPNHARVIDAFLRLGLHNLPNIIWRKPTNAPNKFMGSGMLPPGAYVTLEHEYI
                     LIFRKGDKRVFRSPDEQRRRAESAIFWEERNRWYSDVWSDLTGAPQKLASAARSRSGA
                     FPFELAWRLVNMFSIKGDVVLDPFVGTRTTLLAAAANERHSIGYEIEFGLRPALLRQL
                     GETPGVSRQVLRERIERHLALVGRRTAEGKPPTFTVSRHGWPCVSRQETGMVLRPAAS
                     VRQFSEETTALSVQWEVSYGEIGPGEQVEMPLA"
     gene            21344..22495
                     /gene="trpS"
                     /locus_tag="PKM94_05960"
     CDS             21344..22495
                     /gene="trpS"
                     /locus_tag="PKM94_05960"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD66222.1"
                     /translation="MVNVKSKTTTRPASKNKSDTTRKAVSPESDQAVRETVHAEKEPV
                     TKPRPAKKAKPGAEKEVILSGMQPTGSLHIGNLEGALRNWIELQDQYTMYCCIVDWHA
                     LTAVWEHPEVLRDNIFMMAVDYLASGLDPEKCAIFIQSEVKEHAELHLLFSMLISVPT
                     LMRLPTYQEKAEHLDSYGFLGYPVLQAADICVYNAHKVPVGKDQAKHVWLANDLAKRF
                     NHYYGPTLNEPEGLYREIPLILGSDNRKMSKSYDNHIPLDFTEEETAKRLRTFYTDSQ
                     KIRLGDPGRPEQCPIYLLHRIYTPGAEESVAAPCRSGALGCVECKKRLADNLNAALRP
                     IRARRQELLRRPGDVWDVLADGARRARERAAEVMEKIHTAMKLNYRKQG"
     gene            22499..23296
                     /locus_tag="PKM94_05965"
     CDS             22499..23296
                     /locus_tag="PKM94_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOD66223.1"
                     /translation="MNAPFAAPAENYRVDLPVFNGPLDLLLYLIKKEEVDIYDIPIAR
                     ITQQYLKYMELMKELNLEVAGEFVVMAATLIHIKVRLLLPRDPDQAEEDDPREELIMA
                     LLEYKKYREASEILREKAIMEERYFVPELPVGRPDLKVDLSPGTTLFDLLTAFREVLA
                     NRSDEQVHRVDTFEVSIEDRIGHVMRILRGREAVAFRELFADLPRRIVAVVTFIALLE
                     LVRTRRIAIDQSEPFRELRVYRGEQFDAPQREIDIVDFSRLEVQAAG"
     gene            23303..24121
                     /gene="scpB"
                     /locus_tag="PKM94_05970"
     CDS             23303..24121
                     /gene="scpB"
                     /locus_tag="PKM94_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOD66224.1"
                     /translation="MSEDVFNAAVVEALILASPEPLPARKIAEQLEDLTPAQVGRAVA
                     ALNDRYFQAGASFRIREIAGGFQFYILPEFTGYVEEMFTRRRKMRLTRAALETLAIVA
                     YRQPVTKAEIEHIRGVASDGVLQNLLEKNMVAIRGRAATVGKPLQYGTTDEFLKFFGL
                     AGLDDLPKMKEIEELIRAAEPDNGDQLSFETSVLDVAMARKLNVADGTFDPQTREADP
                     QDDDRPAPPPGAPDNLGAEPPGADDADAEDELGDTALEEAAEQDESDSDAGRHP"
     gene            24153..24875
                     /locus_tag="PKM94_05975"
     CDS             24153..24875
                     /locus_tag="PKM94_05975"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOD66225.1"
                     /translation="MIRINKYLSQCGVTSRRGAEALIKEGRVAVNGRPLTEVGAVIEE
                     ATDEVRVDGEVVAPVEQQVYIVLNKPAEVMTTLRDPFRRRTVARFLKGVPCRVYPVGR
                     LDFDVEGVLLLTNDGDLAYRLTHPKYDVPKVYEAKVEGKFQRVDSEGIARGVALEDGA
                     IGRAAVTVLEYARRTTRVRLTLTEGRKREVKQLCAAVGHPVVYLERVEFAGITAKGLA
                     RGRWRHLTAAEIERLKSLVGLK"
     gene            complement(24940..26595)
                     /locus_tag="PKM94_05980"
     CDS             complement(24940..26595)
                     /locus_tag="PKM94_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66226.1"
                     /translation="MGRHTTTYRAWYAAVLGAYCLYAAIYIARTSFVIDGTRYFTLLD
                     DAMISMRYAENLIRGYGPVFNAGVAPVEGYTNPLWMLYMALVHLLPVGAAKISLVIQI
                     SGLFLGLATLVAVKRLAEALRPHHPLVPLAAVALTAFYLPLNTYNLQGMEVGLLAFGV
                     TLAMWLAARALQGGGALWPLFALLGGLTLVRIDAAIPAAVLTAGVFWALPARRGEVVR
                     WSALCVGGLLAAQTALRYWYYGDLLPNTYYLKMTGYPARLRIAHGWEVLVGFLNRQYW
                     LPFAAPLLAVRWLRHRAYLLLLAVILAQAAYSVYVGGDVWDWWGGANRFISIAMPLLF
                     VALSLALATVAARLRPERKGNGGSLSRPARRLFVAAAAVCLVLLNMLRGPASLSEWAL
                     TSRPLHWTDNANMVRRANLVRELTSENATIAVTWAGAIPYFSGRPTVDLLGKTDRRLA
                     HGSAAERPPFRRWGEFLPGHMKWDYAYSIGVLQPDVIVQLWGSATAAAPWLRDQYDVV
                     PLGPFVFSFRREAPTVHWQRLAELSGRTFSDGRGSIPPSPSDL"
     gene            complement(26758..27261)
                     /locus_tag="PKM94_05985"
     CDS             complement(26758..27261)
                     /locus_tag="PKM94_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD66227.1"
                     /translation="MQVTDIFEYAMKMETDGRDFYLRQAAKADQKQFKQIWEQLADDE
                     LKHFHIFKALRDGQAAEYKEGSATPILATARNVFEDLKAAGRDFTFAGDVEKAWVEAR
                     EVERRSEEFYRDKATQVETEGKRTILNRIADEEHKHFVTLDNVIHFLRRPKEWLEDAE
                     WRHSDEY"
     gene            complement(27498..28499)
                     /locus_tag="PKM94_05990"
     CDS             complement(27498..28499)
                     /locus_tag="PKM94_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD66228.1"
                     /translation="MKCLLPRLIAAAVALMLAAPGGAGAITADHASVAPFGEIPATVI
                     QQVRSQYRIFYGHTSHGSQIVTGMQMIRSEDTLYRFNEGAGTLAITELWTDLGGTGDL
                     AWAEATRSHLNEPGNATNVVVWSWCGGVSTNTPEGIDLYLSEMSALESEYPGVTFVYM
                     TGHLDGTGPTGNLYLRNDQIRSYCTLHDKALFDFADIESYDPEGAYYPEGSDDCAWCA
                     AWCTVNPCPPCDGCAHSHCYNCYRKGKAFWWLLARLAGWQDEPCCTLRGDFTSDGFIK
                     VSDLTALSTYLFRGGPPPACPAHGDATGDGVIRVADLTMLIAYLFRSGPPPSACLPE"
     gene            28653..29660
                     /locus_tag="PKM94_05995"
     CDS             28653..29660
                     /locus_tag="PKM94_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOD66229.1"
                     /translation="MKCHCGYAACRFPAALWFALLAGILAPGGGACAQAAGSVAAPVE
                     RAHFTYYFDQPHYIDLADTALERARRQLFGLVRDTLTYRADVYLVDNADKFQGLVRGR
                     IPDWGAAVAYPSKGRIAVKSPDKFNLGKPLAQLLTHEYAHLVMAQRTGFHEAPRWFDE
                     GLAQRVSTEWSWLDNVAMSKAAVFGQYLPLAEIELMNRFNEDKAHVAYAQSLVAVNYF
                     FDAYGVAAVNVFLDAVREGASYDAALMRATGSDYAGFEEEFRRYWSGRFNLVTLLADM
                     MWLWFALAVIVVVAAFLKYRKRRSYYRKWEREERLQSTDFDYGDPDNPEEVDDDEPWK
                     S"
     gene            29648..>30226
                     /locus_tag="PKM94_06000"
     CDS             29648..>30226
                     /locus_tag="PKM94_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66230.1"
                     /translation="MEKLSYEFEYLLAAAGTGLAQRLSPAAADRFAVTLGDTVHAVWT
                     TRREIARENLRQALGPRMAEPLIRATVKRVFENMARSFIEVSRFERLGPDGARAIIVG
                     DGLAHIEQARAKGRGCVFITAHYGNWELLGNWPALMGHPTVLLVGVQHNPKIHALFNR
                     FRQTLGITTIETGVAARGVLRALRENKVVGIAG"
BASE COUNT         5414 a        10201 c         9612 g         4999 t
ORIGIN      
        1 cagcaggttg cgctgcggat caaaggcgat gccgccggtg aaaccttcgg tcccagcgta
       61 gccgccggtc gaagcataca ccgtcgcggc ctgcaggcca cccgctgccg acaactgcca
      121 caccatggcg ccggtggccg cgtcgatcgc gtacacgtcg ccctcgacgc ccgacgcctg
      181 cgttccgaag aacagcaagc tgccgttggt cgccagcgag ctgtgacacg gcttgcccgg
      241 gttgaacggg ttcaccgccc agcccccata cagcgcgccg gtcaacgcgt cggccgctac
      301 cagacggccg ccgtcggtcg tccagtacac aacgtccgtc ccgccctggt tcagcacgac
      361 aaacacgccg aagcgcgtgt cgccgaacac gccggtggga cccatgttgc cgatatggcg
      421 cgaccagagc aaggcgtagg tgttgaagtt gtaagccagg acctcgtcgt tcgtcccgcc
      481 ggtcacgaag agcacgggat ccgggtaacc ggcaatcgtg gttaccgtcg gcgtgcaccg
      541 aatgtcgccg ggagcgaacg gacccgcctg cggcgtgatc gtcgtcagaa gggcgcccga
      601 gatcagatcg aacaccttgt agttgttcag gaacgaacag accaccttac catcgtacac
      661 caccgggccg gcaaagccaa ccggggtggc cgcggggtag gcccaattca atgtcagatc
      721 gcaccaggca tccgacatct gaacaaacga ggcgttcgag cgattgtacc cgtagccgcg
      781 cgtaaaccag tcggtcggcg tggtgcaggt acgctcgctg tacggaatgc agcaatgctc
      841 ggactcaatg accatcgcca ggtcttcgta cccggcgccg aagccccagt caacatacat
      901 gaggccccag tcgccccagt actccgcata gccttccatg cagccgccgc cgccggcgtc
      961 ggacagcacg gaaataccgg tcagtgtgtc cgcagcatcc gaaatcacgc ccacccagta
     1021 gtcgccgacg acggtgatgc cgcccatgtc atacgtggcc cagtgcgtga ctgaagccgt
     1081 cggagtcggg gtggcgatgc cgtaatcggc cgggttgacg gtgagggagc cgagttcagc
     1141 gcccggcgcg ccgcccgcat ccgcccacac cttgatgtaa gacggagtcg tgtagcgcgg
     1201 ccagctgtac tgcgcgcggt tccagtacca ggcgatcgtc actgcatcga cccggcactc
     1261 ctcgccaacc gaggagaact tctgcgcctc tccgtaatcg ccgtagcgat ccggcagctg
     1321 ccagtagtac gcagtgccgt tgtagcagta gttgatcgag cagtcggagt actcgtcctt
     1381 gcagaggttc gcaacccagt agaactgggc gtccataccc cagccatcga ggaacgagaa
     1441 ccattcgtac ccggttcctc catcgaagta ggacgaagac cgcccgcttc catccgtacc
     1501 atcagacgac aagcaggact cccagctcgt ggcaaagacg ccgttggtcc cgaaggacac
     1561 gtggaagtcg ttggtcagta cgaagttggc cggcatcggg acattctgca gagccgggta
     1621 caaggcatag gtgccggccg gcagagtcac cgtcgtcagc agatcgccgg gaaagccggc
     1681 gttgtcgtcg tagacgcgga tgtaaatgtc attgttacca acgggatcgg tgaccgagcc
     1741 ccaggtcgcg ccgccaccat agatggcgat gtcgatcgag gtaagggtct ccggcccgcc
     1801 cacgtcgaac ttctcagcgt actgctcgtc cccgtagacc gggtgcggcg cgcgccagaa
     1861 gtacgcgacg ttctcaatgt aagcttgtga atagcaatcg gtaaagggta tttcggcgca
     1921 acaacgttcc gcttggataa agaacgaaac gtcgaggccc cagtcgccta acatcgtccc
     1981 ccaggaagtc ccgtagtact cgctggcgcg ttcttcaccg gcaaacggac catcagcagc
     2041 gtccgaaatg acggcgacga ccgaaccggc ctgatcgagc tgcgtgaacc cgaggtgata
     2101 gttcacacca tcggagaaca cccagtccgc agcggagaag tccgctgaga gccagtacag
     2161 accgctgccg ccggccggcg gagtcgcaat atccaccgag tcgagtttgt ttcctggaaa
     2221 gccaaaacca tcgtcgtccc agaggtagac tctcagtgtc ggcgacccaa cctgtgcgtc
     2281 ggcgtacata agaagccagg ccaccttcag agtacagtca tacccggcgt cggccgtgaa
     2341 gcgcatgttg aacaggtcgt ccccgtacgc atccggaatc gtccagaagt aagcgggcga
     2401 accgctcgtg taatcctgga cgtcgcagaa gtaatccagc ttgagagccg gaacctgcag
     2461 accgggcggc aggagatgct gctcgagagc ttctgccggt ttcttgaacg ccgaatgcgc
     2521 cggcccggcc gtcgtgacat catccaaacg cggatggttg ggcacgatct tctcgaccgg
     2581 attcagagca gacgacggct tggctacgtc agaaagtgct gtaccgctaa ggcctacgac
     2641 cagacccaag acgaatactg ctatgaacag tctttttaac atttatttcc tccttaagca
     2701 aggaaactgg atgatttgtt tgccaaagac tgacggcttg ccgagcatac cgattctagt
     2761 atcggcagcc atgaggcatt ccgccatgtt cccgccggtt ggtctaggga aactcgggaa
     2821 caaatctttg acactcagca tcatcttctt ccttcctatc tcttggagtc ggagcttggg
     2881 gctccggtgg agttgtgtta aaaagaactt atgcttctgg gctcgcgaac taatctggtc
     2941 tgtgtatcag tatgtcacct cctgttgccc tgccacgtca acaacggttg cccgccgcct
     3001 cgtggagatg tcaaggtaaa ggaaatagtg agaagacgat tggacgctgg cccgcagtag
     3061 tgtcgtaaac agctgtccgg gaagctccgc ccgaaaagaa cagaaccacc caaacaagca
     3121 tgacgggacc accgaagtag gcccgaggtg cgttaactac tgcaaatccg gtaaagaggt
     3181 ttcgcgcggt ttgccaagcg acgaattcat agtgtggctc tcgaactcac caccgggcgt
     3241 gacacacgct cgatgccaaa atacgaaatc ctcccgtttt gtcaaggcct aaaacccgct
     3301 ccgggagccc aaaaactgca cagccgggaa gaaacgggat actttttgtg gtatctcccg
     3361 ccagatgtcc cccgaccccc cgtcgattgc catgtcactt tcagcgtccc cccgccccgc
     3421 cccccgacta acaacttgac ttgaccccgc ccgccgtctt ctttctcgcc atgcgcctga
     3481 gttcacttaa aatccgaaat ttccgggtcc tgaaatcggt cgacctgact ttcgcggacg
     3541 aagtgatcgg catcaccggc ggcaacggcg ccggcaagtc ctccctggtc gaggcgatcg
     3601 cctgggccct ctacggcaac caggcggccc gctccaggcg ggaagagatc aaggcggctt
     3661 tcgctcgtcc cgaagacttg tgcgaagtat ccctcgaatt ccttattaat gacgaaaagt
     3721 accgtgtaat ccgccgcctc gtcgggaaat cggagcggcc ggaagtcgag ctctaccggg
     3781 gggaatcctc ggagtcggtc ggcgtcaacg agacccggaa atacgtgggg gagctgcttg
     3841 gtctggactg gcggggtttt ctgacctcgt ttctcgcccg ccagcaggag ctcaacgccc
     3901 tctccgtcct gcagccggct cggaggaaag accatctggc cgggatgctg ggaatcgacc
     3961 ggctcgagcg ggcgattgtc cgggtcaagg aagatgtgaa aatcgcgggc gggcaaaccg
     4021 agctcctggc ccgccaggtg gccgacggag agagcctcac tacggcgatc gcccagttga
     4081 cggcccgccg atccgaactg gaagggctcc agtccgctgc tgcggccgag aggaaagcgg
     4141 ccgaggaggc gtttgccgcg gcccgcgccg agtatatcaa agcccaggag acccgggccg
     4201 cctggagcga actcggggcg gggctcgagg ccgcccgcaa gaccgccgcc gcgctggcgg
     4261 tccgcctcaa agaactcgag gccgacaccg ttcagttggc ccaggcggac tcggccgcgg
     4321 ccgaagtccg ggcccgtata gccgctcttg agccggcccg gcgggaactc gaagtcctgt
     4381 ccgaggcccg cgcccgccgc caactgctcg accagatgga gcggcagaga accgaacttc
     4441 aggcggaact cgaccagacc gcgcgggcgg tcgaggaatc ggcccgcgcg ctggccgggc
     4501 tggaatccga cctcgcggcc attcccgcat cgatcaccgc cgccgccgag gccggcaaag
     4561 ccgacctcga cgccgcccgg caggagtacg gccgcctgct ggccgaacag aagtcaatcg
     4621 aggtccagaa agagaaactg gcggcgcaga tgcggtcgat cgccagcctc ggccccgatt
     4681 ccgtctgcga ccggtgcctc cggccgtttg gcgatgatct cccggcgatc cgccggcacc
     4741 tggccgagga gctcgagcag ttgaccgccg cctttgccgg gctcggccgg gagctcgcct
     4801 ccgccgccgc ccgcggcacg cagctcaaac agcaggccga ggcgctggag gctcgctcgc
     4861 tggcccggcg ggaactcgtg atcaggcggg aagccgccgc ccgggagcgc gaagcgctct
     4921 cggcgcgtct ggccgagctc gggcggaggg gagaggcggc ccgggtcaag atcgccgaaa
     4981 tcggcgaggt ggtgttcgac gaggaccggt ttgcccaggc gcagcaagcg gtcgcggcgc
     5041 tcgaagccga gcaccgacaa ctccaccgcc tcgaggggca actggcgcgg cgggaggcgg
     5101 tcaccgccgc ccgcgcccgc accgacgcgg cgctcgtcca ggcccaggcc gaggccgata
     5161 ccctcctgca gcggcgcgac ggcctcggct acgccgagga gacattcgcc gcctgccggg
     5221 aacggttcga ggcggcgcag gcgcggttcg aaacggcccg cgaggcggcc gtcggggcgg
     5281 tcaaggaatt cgaactgacc ggccgggagc tcgacggcaa gagggagcaa ctggcccgcc
     5341 tggaggccgc ccgcgcccag ctagaccagt cgcgcgcccg ccagtactac ggcgagaaac
     5401 tggccgggct gttcgccgaa ttccgcgaac tcctcatctc ccgcatccgc ccgaccctct
     5461 ccgatatctc cggccgcctg ctggccgaga tgaccgacgg gcgctactcg atggtcgagc
     5521 tcgatgagaa gtacaacctg caggtgctcg acgacgggca gttttacggc atcgaccgct
     5581 tctccggcgg cgaaaaggat ctggccaacc tctgcctccg cctggcgatc tcggaggcgc
     5641 tgctccagtc agccggcatc gaccgctcct tcattatcct cgacgaggtg ttcgggtcgc
     5701 aggacgacgg gcgcaaggag ctgatcgtac aggcgctcac caacctcact caccgttttc
     5761 cccagatatt cctcatcacc cacgtgggcg atatccggga ccgcgtcgag tgcctgatcg
     5821 aggttgcgcc caccgggctg gggtggtcga aggtgacggt gcgtggcgag gaaacggaag
     5881 cataaagccc ggctggccgg acggctggcg ctccttttgg tcctgatcgc ggcggtcctg
     5941 atagtccgcc gctcggacat cggccgcgac ccggcgccgg tccccctcac cacggtggtg
     6001 aagataatcg acggcgacac catggtgctc ggcaacggcg agacggtccg cctgctggca
     6061 atcgacacgc ccgaagacgg ggagccgctc cacgacgagg cggccgcgct gctcgcgcaa
     6121 gtcgctctcg gcaaagaagc ccgcatcgtt tcttccggcc gccggcgcga cacctacggc
     6181 cgcctcctgg cgtacgtgta cgtcgacgat accctgctgg tgaaccgggt gcttgtcgac
     6241 agcggcctcg cttacgtcta cctgttccgg gacaccgaca ccgggcgccc cgagaccgac
     6301 cggatcatcg ccgcccagcg agccgcgctc gaccgccggg cggggctgtt cgcgctcccg
     6361 cgggccgcgg agccctacta tgtcgccggc cgaacctcct accgcttcca tcgtcccggc
     6421 tgcccctcga ccgccgaatt cacgtccggc cgggcccgca cttttgcgac ccgccgcgac
     6481 gccctggccg agggactctc gccatgccgt cgctgcaaac cctgagcgcc gcgcccggac
     6541 ggccgttaag gccgcggacc aatcggtcga ttatcgccat tgacagcctc tgtaaggaga
     6601 ctatctttct cttggcggcg ggttctcccg caacccgccc gccgcgcttc gtccgatgca
     6661 ggaaaccaga gctgtagtgt atagctatca ggaggaggtc ttcatgtctt ttcgcacgat
     6721 cctgccggtg ctgtcggcgg tcgcggttct gctggcggcc gcgacgatgg ccgacgcccc
     6781 ccgcgtgatt tccttccagg gccggctgct cgacgacgcc ggggcgccgc tgcccgacgg
     6841 cccgaagagc atccgcatca tcatctggaa cgaccccgcc tccaccctcc cctcccacga
     6901 agtctggaac tccggtccgc tgacggtgga gacggcgggc gggctgtttt cgctcaatct
     6961 cggagaaacg ccgatgccgc cgctcgggcc ggggttgttg gccgacagcg ggctctggat
     7021 cgggatcacg gtcggggcgg aggccgaggg atccccgcgc atcaagatga cttcatcggc
     7081 tttcgccttc cgcgccaccc ttgccgatac agccgcatac gccgacgagg cgagctatgc
     7141 cggtgaagct gcccatgctg acgaagccaa ccgtgccggc gccgcggttc aagcggacag
     7201 tgctgggtat gccgacacgg ccgcgtacgc cttttccgcg cctgtatcgg ccgtcgacca
     7261 cgggagcctg gccgggctcg ccgatgatga ccacccgcag tacctgcgcg ccgacggcgg
     7321 ccgaatgatg accggggatc tcgacctcgg aaagcaccag atccacagtc tggaggcggg
     7381 gaccacgtca tcggacgccg tgcgctatga ccaggcggtg aaggatgggg atgccgcggg
     7441 cggcgacctg accggaacct atccggcccc ccagttggcg gccgatgcgg tgggtaccgc
     7501 ggagatcgcc gccggcgccg tcggcaccag cgaggtggcc gacaactccc ttaccgccac
     7561 cgacctggcg agcagctcgg tcagtacggc cgaagtaaca aacaactccc tgctccacga
     7621 tgacctcgcc accggagcgg tccgcagcga tgaaatccag gacggggcga tcgtcaacgc
     7681 cgatatcaac gcggcggccg ccatcgcccc gtccaagatc gccggaacgg cctgtgatct
     7741 tgcgacggac caggccatca cggggaacaa gacctttcgg gccgcgacgt atttctatga
     7801 cagcacgatg gcggtcggca gttactccgt tttgatcggg accgattcca tcacgccgca
     7861 gtggacatac ccgctgcagg taagccggaa atactccact tcgaacgtga cccacggtat
     7921 cgatgtccac ctgcgcaacg agggaatcgg gaacatctgg ggctactatt cccgggttgg
     7981 cgtcaacgac gccagctccg gtacgcgcag cggaattcag gtcacggcgg gtgattcctc
     8041 caacactgtg ggtaccagtt acggcgtgcg cacctacgcc tacggcggcg accagacgta
     8101 cggcatctac gcacgggcca acgcgggcga catcgtcaat tacgcagggc tgttcagcgg
     8161 caacgtcgat gttataggaa cgctctccaa agatgccggc gccttccgaa tcgaccaccc
     8221 cctcgacccg gacaacaagt acctccagca ctccttcgtc gaatcccccg acatgatgaa
     8281 cgtctacaac ggcaacgtga ccaccgatgc ccagggcgat gccgcggtgg cgctccccga
     8341 ctacttcgag tccctcaaca aggactttcg ctaccagctc acggtcatcg gccagttcgc
     8401 ccaggccatg gttgccagcg agatttccgg cggccgtttc acgattcgga ccgacaagcc
     8461 gaacgtgaaa gtctcctggc aggtgaccgg catccggaaa gacccgtacg cggaggctca
     8521 ccgcatccag gtggaggtcg agaagccgcc gtacgaacgg ggttcctacc gcttccccga
     8581 gggcttcggc ctggggagcg agcggtcggt cgatcgggcc aatgacgcgg agttggagaa
     8641 ttcggcgccg gccccggccg cgcagtaaga tgtgcgccag cgccatggac aacggcggac
     8701 ccgtcggggt ccgccgttca ctttttccgg atgggaatgc ggctcagttg aggaatgtcc
     8761 agcccacggt gaccttgaaa tccggggcgt cggccaggcc gatcttcccc acaatgaaaa
     8821 accggtcgcc gctggccaga ccgcgctcga tgccgccgat caggttcacg cccagctccg
     8881 tctccgaatg attcccgact ccttcgatat cgaagctgta aatgttgacg ccgagaccgc
     8941 cgccgagata gggcgaccag acgtcccaat tcgacgcaaa ccggtagagg gcgtcgaaat
     9001 tgatcgccgc cagagtcacg ttgtcgccga agccgagttc gaaattcggc tcgaagcgga
     9061 tgtgctcggc gaaattgccc acgtcgacgt ggaggccgaa atggacctgg tcggggtcga
     9121 ccgtggcgcc caggcgtggg ccccagccgc gaaaaccggg ctgattgtat gattcctggg
     9181 ccagcgcacc cgggacggcc agcgccgcca gcaacaggat cgccaggctg ccgcacaaag
     9241 cgttcttcat cgtgattctc ctccgtgaag gattgctggg gactagtatc ggcatttcgc
     9301 cggctgccgt tagaagaatg tccacccgat cgtcagcttc aaatccgggg cgtcgaccag
     9361 gccgatcctg gcctccccga aaaagtgtgc gccgccgcgc agccgccagt cgagcccgcc
     9421 cagcaggttg actcccggct cgacgtcgga gccgtcgccc tcgtcgcggt catcgcgccg
     9481 gcgcgccgaa acgccccgat cgaaatcgta atagttggcc ccgattcccg ccccgagata
     9541 cggcatcagg cgcggatcgc gggtccgaag gagacacacc gcgtcgaaat tgagcgctat
     9601 cgcggtgacg ttgtccccga acccgaggtc gaatgcgggg cggaaccgca ctcccggttg
     9661 cggcgggccg acctgcaagt gcacacccag gtgaatctga tccgggtcaa ccgtcaggcc
     9721 gacatggggt ccccagccga gcgcggtcgt tcccgatggc cccgtcggag ctgccgcccc
     9781 ggcgggcgca aggagcatca gcgccgggcc aaaacacagg aagataaggc gggccgtcat
     9841 catggaggat ctcctttccg tcaatctcgt tcgcgattgc cgggttctcg tcagtccgct
     9901 tgcagggaga gaagcgcggc gtggtactgc cgatcaagat acggcgcaag cgcgccgggc
     9961 gcaagggggg cgtcgcggtt cgggggcggt gcccggcggc tcttcggacc ttccctgccc
    10021 aacccttgca caccccgccg cccgcgcccg gcaaattcct cctgccccct ccaaaactat
    10081 aggattcttc cgatatatcc ctcgatagca gattccgaac cagacaggga gccagccgat
    10141 gcccaatatt ctcgtggtcg acgacaagga ctcgatgcgc aacatgctcg cggaaacgct
    10201 cagcgaagag ggccaccggg tcgacgcggc cctggacggc aggagcgcga tcgacctggt
    10261 ccgcaacaag tcctacgatc tcgtcctcac cgacctgaaa atgccggaca tcagcggcct
    10321 cgaggtgctc accacggtga aggaagtgga cagcgaaacc gccgtcatcg tcatgaccgc
    10381 ctacgggacg atcgaggacg cggtggcggc gatgaaagcc ggcgcctacg acttcatcac
    10441 caaacccttc gacaccgagc acctgtgcgt gctgatcaac cgcgctctgg agaaccgccg
    10501 cctcatggcc gaaaacacgc tgctgcggga ggaggcgatg agcggcaagg aattcgccaa
    10561 catcatcgga aagaaccacc agatggccga ggtctgcgcc ctggtgcaga aggtcgccgg
    10621 gtcggacgcc tcggtgctgc tccaggggga gtcgggcacg ggcaaggagc ttttcgcccg
    10681 cgccattcac ggcctctcgt cgcgcgccaa cggcccctac atcaccatca actgcgccgc
    10741 cattccgcgc gaactgctcg agaatgagct gttcggttcc gagaaggggg ccttcaccgg
    10801 cgcccacgcc cggaaaatgg ggaaattcga gattgccaat accgggacga tcttcctcga
    10861 cgaaatcggc gacatggaca tcgccctcca ggcgaaactc ctgcgcgtcc tccagcagcg
    10921 caatttcgaa cgcctcggcg gaaccaaacc gatcgaggtc gacgttcgcg tgatcgccgc
    10981 caccaacatg gatctcaccg aactcatccg cgcaaagcgg ttccgcgagg atctctacta
    11041 ccggctctcg gtttttccgc tcaccatccc gccgctccgc gagcgtctcg acgatctgcc
    11101 gctgctggcc gatttcttca tcgcgaaata ctgcaccgag atgcgcaagc cggtgaaatc
    11161 gctcacccgt ccggccctgg agctgctcca gaaataccac tggccgggga acgtccgcga
    11221 actggaaaac acgatcgaac gcgcggtcat tctcgccgag ggcaagaaaa tcacgcccga
    11281 ccacctggct atccgcctcc gccgcacgag cgaaatccag ttgcgcgacg gcgcggggct
    11341 caaggagatc ggcgccctgg cccagatgca ggccgagcgc agcgtcatcc tccgcgtcct
    11401 taaagacacg cgcggcaata aacgcaaggc cgcccgcatc ctcaagatcg actacaccac
    11461 tctgttcgac aagatcaaga agtacgacat cgagagcgcc ctgaacgaat aggcgcgtcc
    11521 gccggtcgct ccagtaaaat gcgaaaggcc ccgccctccg gcggggcctt tgtgctgact
    11581 gcgccaattg cagaccgtag gtatgcgccg accggccgcc gctggccggc ggcggagtcg
    11641 tcgcgccgaa gcagacgccg tgcaccgccg gtgcgaaagg ggagaggtcg cggacgccga
    11701 acgtcgtgaa ccttcggtgt ccccagattg ccgttcggag tccctgtgca aacacagtcg
    11761 agcaggtggt cgtcacggac ggagccatcg tacatacgcg cggtcgaatg ctcccaaggg
    11821 aagctgctcc ccggcagcct cgccaacagc cggcgcgtca tcctgcggcg gcgctgcgtt
    11881 caacatcctg cgtccttgcc gttccgatcc cggttgtccg cgttggcggc cggtgcgaag
    11941 attccgcttg gcgaacccgc ctgccggcgg taaattgaca gatgtttcag ggggtcgcgc
    12001 agggtgactt ggtgtctgct cgacaacctg ccgcacatcc acagactcag gaggtctcat
    12061 gttcaccacc ggccgcatcc gtcgcttggc ggtgcttgcc cccgtggcgg cactggcgct
    12121 gattttcgcg tgcagcgatt cggggaccga cggtcccccc gtcgagggcg actgcacgac
    12181 tctcaccgcc caggcaaacg ccgccctggc cgccctcatg cagggcgaca tcgacagcat
    12241 catcaatcac ggcgactcga cttttcgccc gcacgacgtc gacttcagca ttccccacgg
    12301 cctctaccag caggccctgg cggccgatcc caactgcgcg gacgccaaga tgggcgcggc
    12361 attcacgggc gtcctcatgt tcatgaccga cgccgacttc aacagcctgg tggagaaatt
    12421 caagcacgcg tacgacacgc tgacgttcaa tccgatgcgc ccgaacagcc tcgcccccgc
    12481 ggtcggtttc ggcggcgaca ttctcaccga tgctattccc ctcaaagcgg ggcgcttcac
    12541 cggcctgatc ccctcgccgc tcgagctcga tcgcgcggcg ctgcggctgg cggcggtcga
    12601 tcccgacctc accgctctgc aggatcggct cgaagagtcg ctcctgccga aaatccggag
    12661 cgcgcgcacc cgcctgctcg acctggtcgc cgatcccgac tacacgttcg agatcacgcc
    12721 ggccatgcag ggcaacgaag gcgccgagcc cgtccttctc gaccggtccg acatccagct
    12781 ctttatcgcc atgacctacg ctgcggaggc ggggctggag gtctttttcg cccgcaacct
    12841 ttccctttcg gcctatacga cggccggcgt tgaagaagcc ctggcccgca cttcggagtt
    12901 cctcagcctc aagtccggcg gcgtcggcac gcagcacatg ggcaacgcca agcagtgcgt
    12961 cctcgatgcc gtcaacgccc tcaagaccgc gatcggctac ctccgcgccg agatcgacac
    13021 ctaccagccc tacgatttca tcgaagtgta cgaggaagac cgcgacgatc tgcaggacgc
    13081 cgagaacgtc gccgattccg tgctcgacta cttcaccggc ccgcgcccgc tgcaggtgac
    13141 cttcaaccgc gagtgggagt gtgtctgggt cgtagaccac gattcctgtt actgggtcga
    13201 ggacacgctg ctcctcgatg tcgacatcgc cgagttcttt gacaatccga tcaccaacta
    13261 caaactcttc gtgcctctgt acacggtcac tgtcgatccc ctgaacgacc tgtacaaggc
    13321 ccacgccgcc gcgcatttca gccgtgcgcg ctactggacg gcgctgggcg ctctccacgg
    13381 cataactgcg cccaatgacg ccgcctactt cggctaccac ctgcccgacg gtgataccga
    13441 tgagttctac cgtttcctcg ccgagtggcc gcagtctcac cagaccgtgt tcgggtggga
    13501 cgacgtggaa aactggtgcg gtccgggcga taccaacctg acgtgctact tctccctcaa
    13561 tgcgcagaac cgttatgaaa ggtactggga ctctcccgat caaatactcg cgtgctacga
    13621 ctgggcagcc gccgacttcg gttcgtggac cttccccaac ccgaccatga acggtctgct
    13681 gccgggcatg acctcggacc gactgaagga aatctggtcc gtcgacctcg gctttccgtg
    13741 gtatccctcc cagtgcgaca ccctggggtt cgacttctga ggggattgac ctctgatggc
    13801 ctcatgtggc gatagtgatg cccggcgctg cgtgccgaag aggagcgccc tggcggcgct
    13861 cctcttgagc ctcctggcgg gtccgcccgc cgccgcggtg cgcctggacg gctcgtcctc
    13921 gggctaccag accatggtgc tcttccgcga ctctcccggc gagcaatggt cggtcggccg
    13981 ctcgcgcatg gaggccgcgg tctcggcgga ccgtttcggc gccggctggc aactgcgcct
    14041 cggccacact gttccgacgg gcgagctgcg cttcggcgac tactccggcc aatggccggc
    14101 cgcggcgccc ggagcggccg accgctttct cggccgcgtc ggaccgcccc gaccgagcac
    14161 cgacgtggcg ctggcggctc gcttctggcg gcggcgcctc gcggtcgagc tcccctgccg
    14221 ctttcgctca accggcgacc ccgactgccg tctcgacgtc tccttttcgc cgctggagtc
    14281 gatccggctc ggcctcgcct gggggcgggt gacgcccgat cccggaccga tcgacctgtt
    14341 ctacgagtat gacgactccg gagcgcgggc ccgcgcgggc ggccgggtgc actggtggag
    14401 ccccgctcgg ctcgcgcagg ccgaagcgga gatcgcccct ctgccgcacc tgcgtctgcg
    14461 ctcggttgtc cgcgaccttg acttcgcgcc cgccacgccg gcattcgacg gtgagcccga
    14521 cggtctctac cgggcctgcc tcgagggttc gctccatgac ggcgcgctcg gcgccgactg
    14581 gcgcctcgcc cggggttggc tcgccgccta cacctaccgg agcctccgcc tcgatgctcg
    14641 cctccagggg tacgacggcg gcgttccctg ggcttacttc ggcatcgtcc gcggcgatct
    14701 gcatcagcac gtcctgagcc tgcaacgggg cgatcgccgt ctgagttggg agtgggggag
    14761 aggcgacggg gccgtccagg ggagcgtcaa actgcgcccc ttcctcgagg ggctctaccg
    14821 gctcctgggc gagcggcggc acatcgtcgc ctcgggcgcg atcgactgga aacatctggc
    14881 cctcgagagc ccgctggtct cgcgcgggtg cgtctccctt cggggcgcgc tcgactggct
    14941 gcgcgccgag ccgagggtcg agtgggtgac ctggcgcccg gtgctggggc tcggcatcga
    15001 cgacctccgc accggccgct gggaccttgt gcgcgccgat ctgctgaggg ttcgcctgct
    15061 gcagacggtg cgcctcggcg cggttgcgct cgatctcgac gtctctcagt ggctccccct
    15121 ctctatcagg cgcgccggcg gagcgtcggc cggatcgggc gcccccggcc cggccgacgg
    15181 gtccgtctcc gaatccgccg gcggcggtct gtggagtggc ttctcgttcg ccgctcgcgt
    15241 gcgtctcata cgttgacccg ccctgttcgg ccgcgccccg cccccaaaaa cctgttgacc
    15301 aaagtccgat cagcccatac attctccccg atgtcatgcc tcgaggtacc cgcgggggcg
    15361 acggcggctc gtgacaggcg cgcgggggga tgccggagtt cccgttccgg cggcctgcgg
    15421 gggagcgcgc tccggtgcgc cgacgaccgc ccggcaccgc cgacccactc cgccgccgga
    15481 tccgtccggg tcgttcgagc gtaaaaggag acagatgcag acatcatcgc tagtcaaccg
    15541 gcgcgttctg cttttgaacc agaactacga accgctctcg gtgtgttcgg ccaagcgggc
    15601 gatcgtcctg gtcatcgagg gtaaagccga gatgatcgag tcggccgacg gtctcaagct
    15661 gcgcaccgtg cggacctcct actccctgcc cagcgtggtt cgcctctgga acttccgcaa
    15721 agtccccctc aaacggatca tgctcacgcg gaaaaacatc attctccgcg acgacggccg
    15781 gtgccagtac tgcggcgcca acaaggggcc gatgacggtc gaccacgtca ttccccggac
    15841 cgaggggggc agcgactcct ggtccaacct tgtgtgcgcc tgtgagcggt gcaacaacct
    15901 gaaaggcaat cggacgccgg agcaagccgg gatggggctg ctgcgaagac cgacccgccc
    15961 gagttacatc acgttcatcc agcgcaacta cggggtcgcc gaccagtggc ggccttacct
    16021 gttcctcgac tacaacaacg gaacgacata cacaacgtag ggggaccggc agggcagagg
    16081 tggcgcagtg cctgatcttc tctggctcac cgacatccat ctcgacttcc ttgataccga
    16141 cggccgacgg cggttcttcc gccggattgg ggagagctgc gccgacggcg tggtggtcac
    16201 gggcgacatc ggggaggcgg ataccgtcga ggggcacctc gccgagctcg cggaggcggc
    16261 cgcgcacctg tccgaggttc tgccgggagc cctcgcccgg tactctcgtc tgctcgtgtt
    16321 gacccatgtg ccgcctttcc gcgaggcctg ccggtacgcc ggacgcatcg cggatggcga
    16381 tcatctgccg cacttctctt cgcaagttgt cggcgaggtt ctggaggcgc acctgcgggc
    16441 caacccctcc cggttcgcga ccgtgctctg cgggcacact cacggcggcg ggaaggcgga
    16501 ggtgcttccg aatctcacgg tgtttacggg agcggccgac tacggccgtc cgcggatcca
    16561 gaaggttctt catctctcct agctccccgc tgccctcttt ctgcacattc gcccggaccc
    16621 cgccaaaatc gccgcgccga ccgtccgata attcgggaaa acagcattcc ttttcacgat
    16681 tgaggataga ggtatgacgc acccgcaagg ccccccccct tacccgaccc aggcctacac
    16741 cgccggatac ggcgacgcgg ccgggagctg ccacacctac gccgccccgc cgccgcccgg
    16801 ctacgcccag ccgctctcgg cccgcgggcg agccgtcgcg accgtctacg ccctcggcgt
    16861 cactctcctg ttccacctga tctcgatccc gctcctgctc tgggagaaca gcctggtcca
    16921 gcgctacggc atggcgatct gggacggcgg cattgcggag ggggaattcg gcgcctgggg
    16981 gctcgtgact ttcggcaacg ccctgttcct gatcgccgcg accgtcacca cggtcgtcct
    17041 ctttctcatg tggatccacc gcgcctaccg ggtgctcgaa gtgaataagg tcgccggcct
    17101 gaaagcgacc gccgggtggg cggtcggctg gtggttcatc ccgatagcca acctggtgaa
    17161 gccgttcgag gtgaccaaag agatcttcgt gcggagcgag cggccgccgg ctggcggagc
    17221 gccctgggga gccggtccga cgccggcccc ggccgggttc gtcgtgagca ccgcccagtc
    17281 gagagaatcc tcgtggatcg tcggcgcctg gtggctgacc tggctggcgg cctcgttcgc
    17341 caccaacctc tccgggtttt tgtcgggggc gttctacgga ctgggcggca acaaccagcc
    17401 gaccgcggag cagtatgtcc gcggcaacct gctggacatc ggcgccaccg tgctgttcat
    17461 tttggcgggc gtactcgcca tcatggtggt ccgcgacatc gaccgccgcc agcaccgcgc
    17521 tttcagccag cagccggccc cgctgcctcc gtcggcctgg ccgcgctgac ctgtggaacc
    17581 ggggccgggc ccgtcggatc cgaactctta tcggcagtga ggtctctatg ccggagagcc
    17641 tgatcgccct tctcgtcttc ggcggctttg ccgtcgtctt tgtcgccgtc atccgggcgg
    17701 cgcgcgcgca gcggcgcgcc cggatcgcgc gcctccagca gatcggcttc ttcgaatgga
    17761 aggaccccga ccaaaccctc gtggcgcgtt ttgtgccgct gatcagcggc ggcggcaaga
    17821 aagccccgat cggcaccgtc ctgcgccgca cctccggcgc gggggagatg tacactttcc
    17881 tgccgcacgg cgacacttct gccgcgggag tgctcatcgg acgggttccc gacctggccc
    17941 ttccccgctt ccgcctgatg cgccgtccgc cgataaacgg cagactcggc acctgggtgg
    18001 tggggctggt cacgaagctg atggggggag acctgcagcc ggtggcgttc gcttcccggc
    18061 ccgatctcgc cgagcagttc cttgccttcg cggagacccc ggccgcgttc gaatcattcg
    18121 cgctgcgcca ctgggcggac ctgctgaaag gcgccccggt cggtttcggc ctcgccggcg
    18181 atcgcgacct gctcgccttt tccttccacg ccgacctcga ccggtcggcc cgccgccgtt
    18241 ccgcactgcc ggcgctccct tcgacagtga caatgacccg ggaccgccaa gatctttgcg
    18301 cccgactggc cgagtcggtg cgccaggcgg agcaattcat cgccgccgcg caaacctcgt
    18361 ggtcggcaca cacagtgtga cgccgcggcg gccgaacgcg acttccccac gaagccggcg
    18421 agacagcagg gctcctcgga aacgcagtcg cacacattac acgcattgaa tccacgcacg
    18481 agcccagggc ggctctccgg aaaatgttaa cagactgcct cgttgcctct taacatttcc
    18541 gcatcgatcg tcaccaaaat tgtcctcgtt tgacaccttc gccggtcgat tttcgtaaca
    18601 ctcttccgcg aaattcgtta tcttacggcg gaactatggc ttctcgttac tgacgaatgt
    18661 aggtgaggtc caatatggcg aaaatgcgat tttccacgat gacgacgcga ctgcgtcgca
    18721 gcgagatccg cgaacttctg aaactcacgc ggacccccgg aacgatctcg ttcggcggcg
    18781 gcctgcccga cccggtggtg tttccccggg aggcggtcga agcggcatcc aagcgggcta
    18841 tccgggaacg cggcgatctc gcactccagt acagtccgac ggaaggggaa ccgttcctca
    18901 aggagcagct cgcggcgtac atgcagcgcc acgacgaaat cgcccgcccc gaggacatgt
    18961 tggttgtctc gtcctcacag caggcgctcg acctgctcgg ccgaatcttc atcgacccgg
    19021 gcgacccggt ccttatcgaa cggcccacct atgtgggctc gatccaggcc tggcgggccc
    19081 tcgacgccga cttccaggga gtcgacatgg atgacgacgg aatcatcccg gagaaacttg
    19141 acgccaagat ccgggagctg gtcgatggcg gccgcccgcc caaattcatc tacgtcatcc
    19201 ccgacttcca gaacccctcc ggaatcaacc tctcgctgga acgccgccac gaggtgctcg
    19261 cgattgcctc gcgttacgat ctgccgatta tcgaagacag cccctaccgc gaactccggt
    19321 tctccggcga gctgatcccg tcgatgtact ccctggatac cgagcggcgg gtgatcctca
    19381 tgaagacgct gtcgaagatt tacacgccgg gattccgcct cggctggatt gtcgctccgc
    19441 ccgacgtgct cgacaagctc atcatggtca aacagggcgc cgacctgtgc acctcggcct
    19501 atgtctcgat catcaccggc tacttgttcg aagacggggc gatcgagcga caggttgagc
    19561 gctgccgcga tctctacgcc cgcaaggccg acgtgatgct ggcggcgatg gagcgcgaga
    19621 tgcccaaaat ccccgggctc tggtggtcca aaccgaccgg cggcatgttc ctctggctgc
    19681 gcatgccgaa tcacatggac accatggaaa tgatccacga tgcgatcaac ctgaaagtcg
    19741 cctacgtggt cggcacagcg ttctacaccg acggctccgg ccgcaacgag atgcggctca
    19801 actactccta cccgaccgaa gagcagatcg aactgggcgt cgaacgtctc ggcaaactca
    19861 tccgcatgcg cgtgcgcggc gaaaagcgcg cgggaaagac gcctgccgac gacaccgacg
    19921 aagaagagtg tgcggcttgc gctgacaccg gcgaccgcac atcggagcag gataggtagg
    19981 gaccatccgc cggtgatatc ggcccgctca cgcaaagtgg cgggccgccc gtcggtcgag
    20041 gcgcccgacg ccgcgccgtc ggcctaggcg agcggcatct ccacctgctc ccccggtccg
    20101 atctcgccgt agcttacctc ccactgcacg gagagcgcgg tcgtctcctc gctgaactgc
    20161 cgcacgctcg ccgccggccg gagcaccatc cccgtctcct ggcggctcac gcacggccac
    20221 ccgtggcggc tcactgtgaa cgtcgggggc ttgccctcgg ccgtccgccg gccgaccagg
    20281 gccaggtggc gctcgatccg ttctctcaac acctgtcgcg agacccccgg cgtttccccg
    20341 agctggcgca gcagcgccgg gcgcagaccg aactcgattt cgtagccgat gctgtggcgt
    20401 tcgttcgccg cggcggccag gagggttgtc ctggtcccga cgaacgggtc gagcacgaca
    20461 tcccccttga tcgagaacat gttgaccagc cgccaggcga gttcgaacgg aaaggcgccg
    20521 ctgcgcgagc gcgccgccga ggccagtttc tgcggcgccc cggtgagatc cgaccagacg
    20581 tcggaatacc accggttgcg ctcctcccag aaaatggcgc tctccgcccg ccgtctctgc
    20641 tcgtcaggac tgcggaacac ccgcttgtcc cccttgcgga atatgaggat gtactcgtgc
    20701 tcgagcgtca cgtaggctcc cggcgggagc atccccgagc ccatgaattt gttcggtgca
    20761 ttggtcggct tgcgccagat aatgttgggg aggttgtgca gcccgagccg gaggaaggca
    20821 tcgatcaccc gggcatggtt gggaaagagg cggaacccgg acccgatact gcgggcggca
    20881 tcgccgatat tgacgcacgc gattcccccc ggcgcgagca cgcgggcgac ttccccccag
    20941 acgcggtcca gcagttggtg catgagggca aaggccgcct ccccttgttc ctgccggaga
    21001 gcatccccaa tcctctcgtc ctgatcgccg aagctgttgt cccacatctc gatcatgggg
    21061 tatggggggg aggttacgac cagcgcggtc ccctcctcgg gcagatcagc cagggcagtc
    21121 gccgggcgaa agtgcacgac gtggcgggtg gtcggggcgc tcgacatgga cggaagatac
    21181 cccggccgcg gcaacggcga aaccggtttt tcctccgtcg taagatgttg cggaccgatc
    21241 ggttatcccg cgacagaatt tagccaaagt tttatttgat ttcccctcgg cccgcgcgta
    21301 agctccggca ccggcgtata acgtccgtgc gtcggttatt aacatggtga acgtgaaaag
    21361 caaaaccacg acccgcccgg cctccaagaa caagtcggac acgacccgca aagcggtgtc
    21421 gccggagtcg gaccaggcag tccgggagac ggtgcacgcc gaaaaggaac cggtgaccaa
    21481 gcccaggccc gctaagaagg ccaaaccggg cgcggagaag gaagtcatcc tgtcggggat
    21541 gcagccgacc ggctcgctcc acatcggcaa cctcgagggg gcgctgcgca actggatcga
    21601 gctgcaggac cagtacacca tgtactgctg catcgtcgac tggcacgccc ttactgccgt
    21661 ctgggaacac ccggaagtgc tgcgcgacaa catcttcatg atggcggtcg attacctcgc
    21721 ctccggcctc gacccggaaa agtgcgccat cttcattcag tcggaagtca aagagcacgc
    21781 cgaactccat ctgctgttct cgatgctcat ctcggtgccg actctgatgc gcctgccgac
    21841 ttaccaggag aaagcggagc accttgattc ctacggtttc ctcggctatc cggtgctgca
    21901 ggcggccgac atctgcgtct acaacgcgca caaggtccct gtcggcaagg accaggccaa
    21961 gcacgtctgg ctggcgaacg atctggccaa gcggttcaac cactactacg gtccgacgct
    22021 gaacgagccg gaggggctgt atcgcgagat tccgctcatc ctcggttcgg acaatcggaa
    22081 gatgtcgaag tcgtacgaca accacatccc gctggatttc acggaagaag agacggccaa
    22141 gcggcttcgg acattctaca cggactcgca gaaaatccgc ctgggcgatc cgggacggcc
    22201 ggagcagtgc ccgatctacc tgctccaccg catctacacg ccgggggccg aggagtcggt
    22261 ggccgcgccc tgccggagcg gggcgctggg ctgtgtcgag tgcaagaagc ggctggccga
    22321 taatctgaat gccgccctgc ggccgatccg cgcccgccgc caggaactgc tgcggcgccc
    22381 cggcgacgtg tgggatgtcc tggccgacgg cgcgcggcgc gcccgcgagc gggccgccga
    22441 ggtcatggag aagatccata ccgccatgaa attgaactac cgaaaacaag gctgaggcat
    22501 gaacgcacca tttgccgcac ccgccgaaaa ctaccgcgtc gacctccccg tcttcaacgg
    22561 tccgctcgac ctgctgctct acctgatcaa aaaggaagag gtcgacatct acgatatccc
    22621 gatcgcccgc atcacccagc agtacctgaa gtacatggag ctgatgaagg agctcaatct
    22681 cgaggtggcg ggcgagttcg tggtcatggc ggcgaccctc attcacatca aggtccgcct
    22741 cctgctcccg cgtgacccgg accaggccga ggaggacgac ccgcgcgaag aactgatcat
    22801 ggccctcctc gagtacaaga agtaccgcga ggcgagcgaa atcctccgcg agaaagcgat
    22861 catggaggag cgctacttcg tccccgagct gccggtcggg cggccggacc tcaaggtgga
    22921 tctctccccg ggcaccaccc tcttcgacct cctcacggcc ttccgggagg tgctcgctaa
    22981 ccgctccgat gaacaggtcc accgggtcga cactttcgag gtgtcgatcg aggaccgcat
    23041 cggccacgtc atgcgcatcc tgcgcgggcg cgaggcggtg gcgttccgcg agttgttcgc
    23101 cgatctgccc cgccgcatcg tcgccgtcgt caccttcatc gccctcctcg agctcgtgcg
    23161 cacgcgccgg atcgccatcg accagtcgga accgttccgc gagctccgcg tgtaccgggg
    23221 cgagcagttc gatgcgccgc agcgggagat cgacatcgtc gacttcagcc ggctcgaggt
    23281 gcaggcggcg ggatagcgac ctatgagcga ggacgtcttt aacgccgcgg tggtcgaagc
    23341 gctgatcctg gcctctcccg aaccgctgcc ggcccgcaaa atcgccgagc agctcgaaga
    23401 cctgacgccg gcgcaggtcg gccgggcggt cgccgcactc aacgaccgct acttccaggc
    23461 cggggcctcc ttccggatcc gcgaaatcgc cggcggcttc cagttctaca tcctccccga
    23521 attcaccggc tacgtcgaag agatgttcac gcgccgccgc aaaatgcgcc tcacccgcgc
    23581 cgccctcgaa accctcgcga tcgtcgccta ccgccagccg gtcaccaaag ccgagatcga
    23641 acacatccgc ggcgtcgcct ccgacggcgt gctgcagaac ctgctggaaa aaaacatggt
    23701 cgcgattcgc ggtcgcgccg ccaccgtcgg gaaaccgctc cagtacggca ccaccgacga
    23761 attcctcaaa ttcttcgggc tggccggcct tgacgatctg cctaagatga aagaaatcga
    23821 ggaattgatc cgggccgccg aaccggacaa cggcgaccag ctcagtttcg agacctcggt
    23881 gctcgacgtg gcaatggcgc ggaaactcaa cgtggccgac ggcaccttcg acccgcagac
    23941 gcgggaggcc gacccgcagg acgatgaccg gccggcgccc ccgcccggcg cgccggacaa
    24001 cctcggcgcg gagcctcccg gggcggacga tgccgacgcc gaagacgagc tcggcgacac
    24061 cgcgctcgaa gaggccgccg agcaggatga gtccgacagc gacgcgggcc ggcatccctg
    24121 acaacccggg cggccgccgg agggctgcat cagtgatcag gatcaacaaa tacctctcgc
    24181 aatgcggggt cacctcgcgg cggggcgccg aggcgctcat caaggagggc cgcgtcgccg
    24241 tcaacggccg gccgctcacc gaggtcggag ccgtcatcga ggaggcgacc gatgaggtgc
    24301 gggtcgacgg cgaggtcgtc gcccctgtcg agcagcaggt ctacatcgtt ctgaacaaac
    24361 ccgccgaggt gatgaccacg ctgcgcgacc cgttccgccg ccgcacagtc gcccgcttcc
    24421 tcaagggtgt tccctgccgg gtctatccgg tggggcggct cgatttcgac gtcgagggcg
    24481 tgctcctgct caccaacgac ggggatctcg cctaccggct cacccacccg aaatacgacg
    24541 tgccgaaagt gtacgaggcg aaagtggagg ggaaattcca gcgcgtcgac agcgagggga
    24601 tcgcgcgtgg ggtcgcgctc gaggacgggg ccatcggccg ggccgcggtc acggtgctgg
    24661 aatatgcgcg ccgcaccacc cgcgtccgcc tgacgctcac cgagggacgc aaacgggagg
    24721 tcaagcagtt atgcgccgcg gtcgggcacc cggtcgtcta cctcgagcgc gtggagtttg
    24781 ccgggatcac ggccaagggg ttggcgcgcg gcagatggcg gcacctcacg gccgcggaga
    24841 tcgagcgcct caaaagcctg gtcggcctga aatagccccc ccgatactcc cgatggtccg
    24901 tcccgcccgc cgggcggcag accgcccgtg cggccggttc tacaggtcgg agggggacgg
    24961 ggggatgctg ccgcggccat cggagaacgt ccggccgctg agttcggcca gccgctgcca
    25021 gtgcaccgtc ggggcctctc tgcggaagga gaagacgaac ggtccgagcg gcacgacgtc
    25081 gtactgatcg cgcagccagg gcgcggccgc ggttgctgaa ccccacaatt gcacgatgac
    25141 atcgggctgc agaacgccga tcgagtaggc atagtcccac ttcatgtggc cggggagaaa
    25201 ctcgccccag cggcgaaacg ggggacgttc ggcggccgag ccgtgcgcca gccggcggtc
    25261 ggtcttcccg aggaggtcaa cggtcgggcg gccggagaag tacgggatcg ctccggccca
    25321 ggtcacggcg atggtggcgt tttcgctcgt cagctctctg accagattcg cccggcgcac
    25381 catgttggcg ttgtccgtcc agtgcagggg gcggctggtc agcgcccact cggacaggga
    25441 ggcgggaccg cggagcatgt tgagcagcac cagacagacg gcggcggccg cgacaaacag
    25501 tctccgcgcc ggcctgctga gcgacccgcc gttgcctttc cgctcgggcc gcaggcgcgc
    25561 cgcgaccgtc gcgagggcca ggctcagggc gacgaagagg agcggcatgg ctatgctgat
    25621 gaaccggttc gcgccgcccc accagtccca gacatcgccg ccgacataga cgctgtaggc
    25681 cgcctgggcg aggataacgg cgagcagcag aagataggcg cggtggcgca gccaccgcac
    25741 ggccagcagg ggcgccgcaa acggcagcca gtattgccgg ttgaggaacc cgaccagcac
    25801 ctcccacccg tgcgcgatcc gcaaccgggc cgggtagccg gtcattttca gatagtacgt
    25861 attcgggagg aggtcgccgt agtaccagta gcgcagcgcc gtctgcgcgg cgagcaggcc
    25921 gccgacgcag agcgcgctcc accgcaccac ctctccccgg cgcgccggga gcgcccaaaa
    25981 cacgccggcg gtgaggacgg ccgcgggaat ggctgcgtca atgcgcacga gcgtcaaccc
    26041 gccgagcagc gcgaagagcg gccagagagc gccgcccccc tgcagggccc gcgccgccag
    26101 ccacatcgcg agcgtgaccc cgaacgccag caggcccacc tccattccct gcaggttgta
    26161 ggtgttgagg ggcagataga atgcggtcag ggcgacggcg gcgagcggga ccaggggatg
    26221 gtgcggacgc agcgcctcgg ccagccgctt caccgcgacc agcgtcgcca gccccaaaaa
    26281 gaggccggag atctggatga caagagagat cttggctgcg cccaccggca gcaggtgcac
    26341 cagcgccatg tagagcatcc acagcggatt ggtgtacccc tcgaccggcg ccacgccggc
    26401 gttgaacacc gggccgtacc cccggatgag gttttcggcg tagcgcatcg agatcatggc
    26461 atcgtcgagc agcgtgaaat agcgcgtgcc gtcgatgacg aaactcgtgc gggcgatata
    26521 gatcgccgcg tagaggcagt acgccccgag aacggcggca taccaggccc gataggtggt
    26581 tgtgtgtcgg cccataccaa aaatacgtac gctgcgcaaa gacggctgtt ggcaccggcg
    26641 gccgcccttc ggacgccgcc gcctgtcacc attatcggcc ggtatgggcg ggagcttggg
    26701 gacaaaacgg ggcgccctac aggcgccccg ccaaatgatc ggttgaccgg aacgagtcta
    26761 gtactcgtcg gagtgacgcc actcggcgtc ctcgagccac tctttcgggc ggcggaggaa
    26821 gtggatgacg ttgtcgaggg ttacgaaatg cttgtgctct tcatcggcga tccggttgag
    26881 gatcgtgcgc ttgccctcgg tctcgacctg cgtggctttg tcgcggtaga actcctcgct
    26941 ccgccgctcc acctcgcggg cttcgaccca ggctttctcg acgtctccgg cgaaggtgaa
    27001 atcgcggccc gcggctttca ggtcctcgaa cacgttgcgc gcggtggcga gaatcggcgt
    27061 tgcgctcccc tctttgtact ccgccgcctg gccgtcgcga agcgccttaa aaatgtggaa
    27121 atgtttgagc tcgtcgtcgg cgagctgctc ccagatctgc ttgaactgct tctggtcggc
    27181 cttggcggcc tggcgaaggt aaaaatcgcg gccgtcggtt tccatcttca tggcgtattc
    27241 aaaaatgtcc gtcacctgca tcgctggcat ctcctttggg tttcaggccc ggtcaattct
    27301 gccggagggt acgaaaatcg cgggtggaag tcaactgcca tgcgccgcgc cggccgacca
    27361 gggatgacag ctcgcgtctg cggcgtctgt tgaaagccaa ggggttcaag agaatcggcc
    27421 ccgccccgcg ccttttgccg tcggcgccgc ccacggatgg ggaagcagtc gaaagccacc
    27481 cgccgtcgaa acgacactca ctcgggcagg caggccgagg gcggcgggcc ggagcggaag
    27541 agatacgcga tgagcatggt caggtcagcc actctgataa caccgtctcc ggtggcgtcg
    27601 ccgtgggccg ggcaggccgg gggcggcccc ccgcggaaca ggtaggtgct gagcgccgtg
    27661 agatcgctta ccttaatgaa accgtcgctg gtgaaatcgc cgcgcagggt gcagcacggc
    27721 tcgtcctgcc acccggcgag gcgcgccagc agccaccaga acgccttgcc cttccggtag
    27781 cagttgtagc agtgcgagtg ggcgcagccg tcgcacggcg ggcaggggtt gacggtgcac
    27841 caggcggcgc accatgcgca gtcgtcggag ccctcgggat agtacgcgcc ctccgggtcg
    27901 tagctctcga tgtcggcaaa gtcgaacagc gctttgtcgt gcagcgtgca gtagcttcga
    27961 atctgatcgt tgcggagata gaggttgccg gtcgggccgg tgccgtcgag atgccccgtc
    28021 atatagacga aggtgacgcc cgggtactcg ctttccagcg cggacatctc actcaggtag
    28081 aggtcgattc cctccggcgt gttggtcgac acgcccccgc accacgacca cacgaccaca
    28141 ttcgtcgcat tgcccggttc attgagatgg ctgcgggtcg cctcggccca ggccaggtcg
    28201 ccggtgcctc ccagatcagt ccagagctcg gtgatagcga gtgtccccgc gccttcgttg
    28261 aaccgataga gggtgtcttc ggaccggatc atctgcattc cggtgacgat ctggctcccg
    28321 tgcgatgtgt ggccgtagaa aatccggtac tggctgcgca cctgctggat gacggtagcc
    28381 gggatttcgc caaacggggc cacactggcg tggtcggcgg tgatggcgcc ggcgccgccg
    28441 ggcgcggcga gcatgagcgc gaccgcggcc gcgatgaggc gaggcaatag gcacttcata
    28501 cgacatcccc ttgtcatgac ggcggtccct taccgaatgc actatagtca aaagatagcg
    28561 tccggacgcc gccctgtcaa tcggatagac ggcgccggcg gcgctttccg cgccacttgc
    28621 cctcacccgc ccgtttgcct atacttggcc cgatgaaatg tcactgcgga tacgccgcgt
    28681 gccgatttcc ggccgccctg tggtttgctc tgctggccgg gattctcgca cccggcgggg
    28741 gcgcttgcgc gcaggcggcg gggtcggtcg ctgcgccggt ggaacgggcg cacttcacct
    28801 actacttcga tcagccgcac tacatcgacc tggccgatac ggccctggag cgggcccggc
    28861 gacagctgtt cgggctggtg cgcgatacct tgacctacag ggcggatgtc tatctggtcg
    28921 ataatgccga caagttccag ggtctcgtgc gggggcgtat cccggactgg ggggcggcgg
    28981 tggcgtaccc gtccaaaggg cggatcgcgg tgaaatcgcc ggacaagttc aatctcggca
    29041 aaccgctcgc gcagttgctc acgcacgaat atgcccacct cgtcatggcg cagcggacgg
    29101 ggttccacga ggcgccgcgc tggtttgacg aggggctcgc ccagcgggtg tcgaccgagt
    29161 ggtcgtggct ggacaacgtg gcgatgagca aagcggccgt gttcgggcag tatctcccgc
    29221 tggccgagat cgaattgatg aaccgcttca acgaggacaa ggcccacgtt gcctacgccc
    29281 agtcgctcgt ggcggtcaac tatttcttcg acgcctacgg ggtggcggcg gtcaacgtgt
    29341 tcctcgacgc ggtacgcgag ggggcctcgt atgacgcggc cctcatgcgg gcgaccggat
    29401 cggactacgc cgggttcgag gaggagttcc gcaggtactg gagcggccgc ttcaacctcg
    29461 tcaccctgct ggccgacatg atgtggctgt ggtttgccct ggcggtcatc gtcgtggttg
    29521 cggccttcct gaagtaccgc aagcgccgga gctactaccg gaagtgggag cgggaggagc
    29581 ggctgcagtc gaccgatttc gactacggcg atccggacaa tcccgaggag gtcgatgacg
    29641 acgagccatg gaaaagctga gctacgagtt cgaatatctg ctggcggccg ccgggaccgg
    29701 gcttgcacag cggctgtcgc cggcggcggc cgaccggttt gcggtcacgc tcggcgatac
    29761 cgtccatgcg gtttggacga cgcgccggga gatcgcccgg gaaaatctgc ggcaggcgct
    29821 ggggccccgg atggccgagc cattaatccg cgcgaccgtt aagcgtgtct ttgagaatat
    29881 ggcgcggtcg tttattgaag tctcccggtt cgagcgactc gggccggacg gggcgcgggc
    29941 gattatcgtc ggcgacggtc tggctcacat cgaacaggcg cgggcgaagg ggcgcgggtg
    30001 cgtcttcatc accgcccact acggcaactg ggagctctta gggaactggc cggcgctgat
    30061 ggggcacccg acggtgctct tggtgggggt gcagcacaac ccgaagatcc acgcactgtt
    30121 caaccggttc cggcagacgc tggggatcac gacgatcgag acgggggtgg cggcgcgcgg
    30181 tgtgctgcgg gcgctccgcg aaaacaaggt agtggggatc gccggc
//
LOCUS       DAOPOE010000032        29385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2042_length_29385_cov_6.892431, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000032 DAOPOE010000000
VERSION     DAOPOE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29385
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_29385_cov_6.892431"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKM94_06005"
     CDS             complement(<1..207)
                     /locus_tag="PKM94_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOD66231.1"
                     /translation="MKTSDIRRSFLDFFARHDHRVVPSSPVIPFDDPTLLFTNAGMNQ
                     FKDVFTGQRTLPYARATSSQKCIRA"
     gene            complement(203..853)
                     /locus_tag="PKM94_06010"
     CDS             complement(203..853)
                     /locus_tag="PKM94_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOD66232.1"
                     /translation="MDERISLIDIRLVGSRAEIHLSTHERPLVLSPETVHRYRLVKGT
                     VLTRSQAAQLETEAARFAVASEATRLLARRPHSAGELRQKLLRQFDAELVGAAIRDFT
                     ARGVLDDAQYAHQLADSLVRRKPCGRGYVIAFLESRRIERALAAEAADAVLSEHSPEE
                     LAERALRAKWREFGQFDLETARRRAYNYLARRGFPFDIARAAVQTLARESTDGGND"
     gene            1200..2066
                     /locus_tag="PKM94_06015"
     CDS             1200..2066
                     /locus_tag="PKM94_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOD66233.1"
                     /translation="MDLDLQGNIERFTLPEILQLIASGRKSGTLGIQKADSIVMVYFR
                     EGNIIYGYGPRETYHLGQLLKERGLITAEQLEEAVRMQAVTDNSRRLGEILITRGFID
                     RADLQDVVRRQVESLLYSLLSWTSGSFKFYEDQFPTEEEITVDLSVENVILEGLRRID
                     EMNLIRETLPNLEAVYTISAAQGGRARNVALHADEWNIMALVDGHRTLNEVCRLSPLG
                     RDETLRKLAQLKLAGLITKTDRPIEPPAPAADLEKMVGRLAGLLESYMTEKAAPRPAE
                     RRITQSLLEQVD"
     gene            2063..2764
                     /locus_tag="PKM94_06020"
     CDS             2063..2764
                     /locus_tag="PKM94_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66234.1"
                     /translation="MSQIHTLKSLEQVLETFLERVVAYKQPRLQVLEGINRLDDLART
                     PDPGTDFTEQVGEWFAAHNRLLDQKQLRTADLNRIGGILAEIRSRLDLGGDSPPAAHK
                     ISSEIERWRSGAPTAVPPRITLARGPERSSPPAPSPHPPVTDPGDSIARFGDFLTHAQ
                     ALYADTAGNKKHLLSVLDDLLSAATLRQNKDALILSAFIIYYLKLGNYKVEPFVKRLK
                     HAEELVKGGQSHAEA"
     gene            2751..2999
                     /locus_tag="PKM94_06025"
     CDS             2751..2999
                     /locus_tag="PKM94_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66235.1"
                     /translation="MLRPDEKAYCQALLALRQKDYRAALRFFDAAAPGFSDNREFSLY
                     REATRLLLAVKDKLAELENKDTIDTKETVTHGQETELR"
     gene            2974..3159
                     /locus_tag="PKM94_06030"
     CDS             2974..3159
                     /locus_tag="PKM94_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66236.1"
                     /translation="MAKRQSFADKASKKSHQVICPVCESPVQYVKHVKAEKNGAGWKF
                     RAVSVGVCKCNEKEIYA"
     gene            3236..5296
                     /locus_tag="PKM94_06035"
     CDS             3236..5296
                     /locus_tag="PKM94_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66237.1"
                     /translation="MNRPGDTPGPGISFLLATLLTLAVWMLVALFPGGRTWGLAIWAW
                     LPRLPALVVLGLAVLAAVALHVFLRRAPAAAGPSDAPLSVRRRPWLLPVALTGAVALF
                     WWARTRTHFLGDGYTLLSLLAADRPFLKTRQLGEALLHVWVKDLSGLEGQQGALFSFR
                     FLSILSGTVCVGVVAWAAARLFQDRRSRVLFSAGVLTGGYMLLYFGYVENYSLFVTSV
                     CAFVLVGLLVAQGRLWRGWLLVPLGAAIFFHIMGVTLIPGAVYLLLAPTRLGQRARGW
                     PPRTWAALAVLVGAAGFSAVAYFRHTSVFFRLAVLPLYDGRLTNGYTVLSPAHLGDFL
                     NLIPLLLPAAGLLALALVGFVKSRRPVTRPFAFLVVAVLSTLGAAFIFEPELGMPRDW
                     DLFAFAGVPLVVGFYYLVLNGRRERRPTVEVAALGILLGAAALGGRVATLRDEQVSLR
                     PIAHYIALDPARMSPVFHILREYYAARGDALAADSVEAEWRKHNPEAELTSRAVDLIE
                     MGQYAEAAALARRAIEINAAHWSGYAELGAALIRMQRYDSALSCLQMAGGLNPYSPRI
                     LHNLALCYDRTGDWRKAEQLWQKAYRLDTTQTPTLVELCRLYQIRRLPDKLLAALERL
                     ADQPDAPPEGLAKLADMYAEAGRLPEAGEVFRRAVQAGLDTALARQIVAARPRLKSFV
                     PGFP"
     gene            5325..5846
                     /locus_tag="PKM94_06040"
     CDS             5325..5846
                     /locus_tag="PKM94_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOD66238.1"
                     /translation="MEWLPRLWLLSVAALLFQAAYYWPRLPERLASHFDASGVPNGYS
                     SRASFIAVWLAVVLLVNIWPAIIGPLIRKLPPGLINTPRKDYWLATQERRTRLIAIVA
                     AMLSGIMLGANVMLLVTFHYTAAYNTGGRGSSGSWIGYAVVAETLLTLGAVVWGLVTL
                     SRPPADFRPQPPS"
     gene            complement(5824..6564)
                     /locus_tag="PKM94_06045"
     CDS             complement(5824..6564)
                     /locus_tag="PKM94_06045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66239.1"
                     /translation="MLKPEEDAFGRGMCDHLHGRYTSLIVERDDGFIDASHVGHRLCL
                     ERYARWWKVERQAIRYARGRVLDVGCGAGRVALHLQEKGHPVVAIDYSPLLVHVCRER
                     GVRDVRLMRFADVSADMGVFDAVVLFGGNFGLLETLQKGRRMLRRLHGMTSDRGRIIA
                     SVRHPYKVSDVQHLEYHECNRRRGRRGGQLRLRVRYEKLATPWFDYLLVSPEELREIV
                     AGTGWQVSRLFEDESAFYSMVLTKEAAV"
     gene            complement(6593..7594)
                     /gene="tsaD"
                     /locus_tag="PKM94_06050"
     CDS             complement(6593..7594)
                     /gene="tsaD"
                     /locus_tag="PKM94_06050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006438956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOD66240.1"
                     /translation="MLALGIETSCDETAAAVIADGSRVLANVILSQADHARFGGVVPE
                     IASREHLKTIVPIYRQAMETAGVGIGDLGLIAATMGPGLVGPLLVGLTFAKGLAFAAR
                     LPFVPVNHLEGHLAANLLAYADLSPHHLTLVVSGGHTMLIEVNGFGVYRVLGRTQDDA
                     AGEAFDKVAKLMDLGYPGGARLDKLAQQGNPGYVRFPRAMLDREDYHFSFSGLKTAVA
                     LHLKKLSPGELARHKADIAASFQEAVAEVLAEKTLRAARDLQIHDVTVSGGVAANSRL
                     RALLGERLTPEGRRLFSPPPELCTDNAAMIAAAGYFRYRKEGAGEMSANAVPYLRLT"
     gene            complement(7601..8527)
                     /locus_tag="PKM94_06055"
     CDS             complement(7601..8527)
                     /locus_tag="PKM94_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66241.1"
                     /translation="MKSLLENFWLKIVALIMGLLLWFHVATEKIYNYEVRVPISEIAL
                     DDSLTLANRPPDSITVVVAAKGKQLLRQTWRDYGVRILATAYGPGRHVVPLSAANTTL
                     IAGGNRVAIDGVVSPTSINLHVDEIVRATVDVVLDLDIKTDEGFAVSRISDPLPPQVV
                     VRGAELVVDRLSAVSTIPRQITGVRNNLSLLLPLRRPEGYRIGLDPDSVLVEVEVLAI
                     KTRVFENIPVVVYNVPADRQVSIQPRVINVELTGPPEDIDLLNPNALVASVDYRRRDS
                     LNAAPIKVDAPSNFRVKKTSAESVQFTTRRPQ"
     gene            complement(8629..10098)
                     /locus_tag="PKM94_06060"
     CDS             complement(8629..10098)
                     /locus_tag="PKM94_06060"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOD66242.1"
                     /translation="MKARTTIAGILAGLLLAVAAHAGYPEGQAVTFSDFSRTRSIAVT
                     VVRVYFATAQGIICYNQGEDRWEEPLPAIPGVDPEDISRIWADQFGRTLYAATPDGRY
                     VYDQTLAMWFLTSETPEGDGAAREVPPPAIMHAPPGFNYFAGGQLIDPYGRTWQINEM
                     VDDGSGMYWMAIWGYGPAKARTTNLIIDLLPFGLVQRRVNALDQEGGRLWVSGAVYDD
                     LRTGITGFEPAMMTFRHLESGVRPEFPADDINCLRSDSAHLYIGTARGLLVMDLESEM
                     IIRTVGERQGLAGENVLSIAVTGDSVFIGTDEGLNLVIGASDSAALILPTTFEGQYIW
                     DIEEVGDYVWVAAESGAYRLTKGAERLQRYRDPENVLFSHVYDIERWADKLWFVSDGG
                     LAELDLQTAESRSFHDMTQQLLPRALAVNETIAAVASDKGLTILFHTADKPFKRDFTV
                     DDGLASNNVYALAVDGDYLWVGTDRGLTRFLWNNPDRVD"
     gene            complement(10095..11297)
                     /locus_tag="PKM94_06065"
     CDS             complement(10095..11297)
                     /locus_tag="PKM94_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOD66243.1"
                     /translation="MPHSPDRPDRILAAAKRIAARQAALRRHLHQYPELSAREFRTSE
                     YLAAALRRLGLRLLATTTDTGVLAELQGVAGGRTVVVRSDIDALPITERTGLPFASKN
                     PGCMHACGHDMHMAVVWGVAAVLSGLRGQIAGTVRFIFQPSEEMPPGGARPMIESGAL
                     TDASAVFGLHVDPHVRTGKIGLRDGPTMAAVTDFDLIVRGRSAHAARPHLGVDAITTA
                     AEVIEAVQKVVAREIDPVEPAVITFGQIEGGVARNVVADEVRVAGTARTLSPRAEKAV
                     PRLIRRVAENIGRARGARVELRLIADYPVLSNDPRANRVLARNMERLYGRGKIETTEV
                     MLGGEDFACYLKQAPGAMVRLGVGNRKIKADQPWHSPAFIADEAALPVGTALLAAAVL
                     DVLSGGLA"
     gene            complement(11300..12025)
                     /locus_tag="PKM94_06070"
     CDS             complement(11300..12025)
                     /locus_tag="PKM94_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOD66244.1"
                     /translation="MQYRQIEKWPVTRAEAAALQTALAREIRIAPAASEPRLIAAVDT
                     AYGRDGKRIYAAAVVVRYPGLEPVDRAEGCAPAIFPYVPGLLFFREGPAVIAALHRLA
                     TEPDMLMIHGHGLAHPARCGMAGLLGVLFNRPSTGCSRKLLAGTHRPVADAKGSWEPI
                     RIEGAEVGCAYRSKDRVKPIFVSPGHGCDLAGARELVVRCLRDYRLPEPLRLAHVFAN
                     KYKRYQENGTAGRKAGSPPPERT"
     gene            complement(12111..13013)
                     /locus_tag="PKM94_06075"
     CDS             complement(12111..13013)
                     /locus_tag="PKM94_06075"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOD66245.1"
                     /translation="MTRCVAALLAILAAAVPLRDSRGADPAPPAPFDSLLAWYRQTYS
                     FISMDDTIYTFETEFRLPEGFHRPDSAALTGYQYWVSHLPLWNQWKPVGVWKGGKAYE
                     YGEVSRVVHMPWRGTMYTDIGFPLRVFAEYCRYAGRESAMHIVTRADGDTLAYAAWLQ
                     SKVSYTPLGGPRLVPATPREASEMEFYRFLTVAMEQQTYQTLAANCDSLPPEEVRPGD
                     LVIGHDDRLLVGKVYIVVNMLVNDEGERMYTVATGCPKPNACDLHIPLLTPDRHSPWI
                     TPGHLAALVDDLPQWGFFRLRGSQ"
     gene            complement(13010..13354)
                     /locus_tag="PKM94_06080"
     CDS             complement(13010..13354)
                     /locus_tag="PKM94_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOD66246.1"
                     /translation="MSDRVQQFRQEREKLNEIVLERDNLDIKRFFALDGAVYRDGALD
                     AKTKELLGLAASLVLRCDDCIAYHVIRARENRVTDAEFDEVMAIALVVGGSITIPHLR
                     RAARLWKEMKEE"
     gene            complement(13422..15311)
                     /gene="pepF"
                     /locus_tag="PKM94_06085"
     CDS             complement(13422..15311)
                     /gene="pepF"
                     /locus_tag="PKM94_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOD66247.1"
                     /translation="MWLTSRVLSPVLLAVGLFLAAASAVISADSAIPQRADIADQYKW
                     RVADIFPTDSAWEAAFAGLQGSIGLFDQYRGRLGEGPATLLACLATRDSIELIADNLY
                     VYAYLILDEDNRVSTYQEMGGRIRQLWSRISEGTSFIEPEIIAAGKDKIVGFAGQDPK
                     LAVYRHYLDDLFRKQAHVLSDKEEALLALAGPAVSASGEIFNMVDNADIDYGTVADEN
                     GAEVALTKERVYKMLESTDRELRRRVSDRYNAAYLDYENTLAATLAASVKSDYFQAQA
                     RGYATCLESSLDRYNIPVAVFHSLIAAVNENLAPLHKWASLRKRILGVDTLFTFDLSV
                     PLMPEGRKEYTYEQAQEILRRGLQPLGPQFLTDLEKGFTSGWIDVYETEGKGSGAYSW
                     GTYSSHPYVLLNYNGTLASVFTLAHEMGHAMHSYYTNRHEPYISHNHSLFAAEVASTG
                     NEAVLMKYLLDNTTDRGDKMELLNYYIEQIIGSFFTQVMFSEFELTIHERIESGQALS
                     AEFMRKTYRDIYQKYWGPDLTIGAVNDLGCLRIGHFYSQYYVYQYATSYAAAQAMSQK
                     VLEDPKYLDTYMQFLAVGNSDYPVEILKKAGVDLTSPEPVQRTMKLFADLVDQMEALL
                     AESGK"
     gene            15430..15831
                     /locus_tag="PKM94_06090"
     CDS             15430..15831
                     /locus_tag="PKM94_06090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD66248.1"
                     /translation="MYTYRTAVKLHDTDAAGVLFFANYFRLAHEAYEAWMSAVGFDFF
                     HLIHEADYLVLIVRAECDFRRPLRVGDGVTVRMTASGIGRSSFTLAYTVVDDDDRVVA
                     QGATVHACVGKPDLNSVPLPAGLKTELEKLQ"
     gene            complement(15931..17475)
                     /locus_tag="PKM94_06095"
     CDS             complement(15931..17475)
                     /locus_tag="PKM94_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD66249.1"
                     /translation="MTDIPCPLGEAARRRPAAPFLVTPAREYSYAEANRAVNRRAAAL
                     ARLETRLGPLRRLALLVPKAGDEPSLDVIITFLAAGRAGATALVVDGALPAPLVRAAL
                     PAEQGIVAVVAQDRREHIPFAAATWEEVAEIEAQILLSPAEADRTDFRRNLRLEATGV
                     LTSGSTGTPKLCLHTVGNHYYSALGSNENLPLAEGDRWLLSLPLHHVGGIAILYRAML
                     AGAAVVPGRRPANPAESIETLGVTHCSMVPTQLRRYLAEGGHRAGLKAVLIGGAAAPR
                     GLIEAAAARGLPLFTSYGLTEMCSQVTTTAPDDPLRRLFGSGRLLPFRELRIDESGEI
                     LVRGPVLSPGYLESNGLRTIADAEGWFHTGDRGELEPDGYLRVTGRKDHMLISGGENV
                     QPELVEKMLLELPDVAEAVVVAVEDAQYGQRPFAFVSFVRKDPRTYDGTTRLLRDYVL
                     LAGGRADTPAQVREMFRERLPAYAVPVRVALIPEEARAGLKPDRKYLERVAQALYGQA
                     GEKTAG"
     gene            complement(17468..18583)
                     /gene="menC"
                     /locus_tag="PKM94_06100"
     CDS             complement(17468..18583)
                     /gene="menC"
                     /locus_tag="PKM94_06100"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOD66250.1"
                     /translation="MRIAQVQLYRYVLPFRRPPVIRGEELRSREGMLVKVTLEDGATA
                     WGEAAPLPGHSRESLADAERELRELRAGVQGAQIPAGLERLDGGFGNWLGHSWLTASV
                     SVAMETAVLQLVAHRQGKSLHRLLCPNPRERITVNALLSGSADEAFGQAERRKGEGFG
                     AFKLKVGRRPFDEDIEAVRRVRETIGPRAKLRLDANRAFDIPTAIRFGQAAAPYDIEY
                     IEEPVRSFLELLELTHRGGFRLPVALDESLRTISPKALSQVRGVRAIVLKPTLLGFEW
                     AMWFARRARRAGMLAVISSAYESSLTLAMLAEIGACLNDGEAGENVAMGLDTQDALAE
                     DLTVEPLRIAQGQIDLSALPAGTFEVRPDRLGEPAHD"
     gene            complement(18580..19536)
                     /locus_tag="PKM94_06105"
     CDS             complement(18580..19536)
                     /locus_tag="PKM94_06105"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOD66251.1"
                     /translation="MLSRRSANRISASFPGSPDTVAAGAPIGRPQVWLLAARPKTLFA
                     SAAPVVMGTAMAAEAGAFHLWSALAALAGALLIQIGTNFANDYSDFKSGADAIARVGP
                     IRVTQAGLVTPGAMKAATALVFGLAVLCGAYLVYRGGWPVTVIGLSSVLWGILYTAGP
                     YPLGYIGLADLFVFVYFGPVAVGGTYYVQTLAITPPVLVAGAGTGLFSVAILTVNNLR
                     DIESDRRAGKRTLAARFGRTFAQWEYVLAVAIAAGIPAALALLTEGHSSAIIASLTIL
                     PALAAFRTIRRATDGPTLNRLLATTGKLLLLYSLLFSIGWWW"
     gene            complement(19481..20302)
                     /gene="menB"
                     /locus_tag="PKM94_06110"
     CDS             complement(19481..20302)
                     /gene="menB"
                     /locus_tag="PKM94_06110"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOD66252.1"
                     /translation="MARIIWTEAGAYSDITYHKAEGIAKITINRPEVRNAFRPLTVQE
                     MMAALADAREDPQIGVIILTGAGDQAFCSGGDQRIRGDSGYTDTSGVNRLNVLEFQRQ
                     IRTCPKPVVAMVAGYAIGGGHVLHVLCDLTIAADNAVFGQTGPKVGSFDGGYGSSYLA
                     RLVGQKKAREIWFLCRRYDARQALEMGLVNAVVPLARLEEETVQWCREILANSPTAIR
                     CLKAALNADCDGQAGLQELAGNATMLFYMTAEAQEGRDAFKEKRKPDFGKFPRQP"
     gene            complement(20313..21110)
                     /gene="menH"
                     /locus_tag="PKM94_06115"
     CDS             complement(20313..21110)
                     /gene="menH"
                     /locus_tag="PKM94_06115"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HOD66253.1"
                     /translation="MAGDFRFHLETAGSPRNPAVVALHGFMGTGADWSTVMAALAGER
                     YGLAPDLPGHGKTRGPEGAGSWRMEQVAAALALELARRGIAQTDLIGYSMGGRLGLHL
                     ALHCRARVRRLVLESASPGLSDGDERIARRRQDDTLARRLETEPLERFLRDWYDQPMF
                     AGLKRVPEEFAGMLERRRAQDGGALARALREMGTGAQEPQWDRLADAPPTLLIVGAED
                     AKFREIAARMAERMPGARVATVAGAGHAVHVEQPARFVELVQEFLSG"
     gene            complement(21115..22875)
                     /gene="menD"
                     /locus_tag="PKM94_06120"
     CDS             complement(21115..22875)
                     /gene="menD"
                     /locus_tag="PKM94_06120"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOD66254.1"
                     /translation="MNSRFPNLNSLWAEAAVEELVRCGVRLFCLCPGSRSTPLTAAAA
                     RHEAARKIVHFDERGAAFCALGYARATEKPAAVVVTSGTAAANLYPAVIEAAMDGVPL
                     LVLTADRPPELRASGANQTIDQTKLYADYPRWFFDLPCPSRETPPEFVLTTIDQAVYR
                     AQHGPAGPVHLNWMFREPLAPVGAEEDFGDYLRSVGCWRTGESPWTIYRSPVKVCEPA
                     RMEAATGLLNRARRGVIVVGRLRREAERVAIAKVAEALGWPVIADIGSGLRLGAAGHV
                     IAPAEYMLAAESFADALAPDTVLHFGPIVTSKRVAQWLKRSQPAAYIHAAAAPERHDP
                     HHQVTHRFEADIDMFCSFLLPFLREKAKETGLAAWRAGADKAEAAIARVLAEDATLSE
                     PLTARLVAENISAAGALFVASSMPIRDLDALAAGDGPAAPVAANRGASGIDGTVASAA
                     GFGLGWERPVTLLTGDLALLHDLNSLALVRRLAQPMTIVVLNNNGGGIFHFLPIAERA
                     DLFEPYWGTPHGITFKQAAQMFGLSYAHPTSAEEFARIYREAQAGPGGGTLIEVRTDR
                     RRNRQLHARLTEAVVRALEG"
     gene            complement(22872..24257)
                     /locus_tag="PKM94_06125"
     CDS             complement(22872..24257)
                     /locus_tag="PKM94_06125"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOD66255.1"
                     /translation="MMPSVFKDSDTHTPAGAAAHLAEEIARRAAHPRAGSPHVERCEV
                     PLAAMEPAAWLAAQRQSERIFWSDREDRSAVAGVGAAAVEFRAVCADPHDLMGRIREL
                     IGDAEGVRCYGGIRFDSDREQADHWRQFGGARFVLPRFELAARGGAAVLAVNLRFPED
                     RGQVSRIIEDLAQLRFAPADPGIPPAGVRRRVDTPGREDWLDIVGAAVDEINAGRMDK
                     IVLARESRCELGSPADPFRLTARLAASAPGCFVFAFKPPDGAAFLGATPERLYRREGR
                     RITSEALAGTRRRGETPAEDALLGEKLLTSRKEAQEHACVADAVRGALAALCREAAEE
                     KGRELVKLARVQHLATRFAGVLRDGVGDADILARLHPTPAVGGAPTATARQRIRELEA
                     FDRGWYAGPVGWIGADSAEFAVAIRSALAAGDTLRLYSGAGIVGGSHPDREWDEIENK
                     IDNYLKLLAGP"
     gene            complement(24286..25026)
                     /gene="ubiE"
                     /locus_tag="PKM94_06130"
     CDS             complement(24286..25026)
                     /gene="ubiE"
                     /locus_tag="PKM94_06130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOD66256.1"
                     /translation="MAAEDHADKTADPIPEKGESPQMFDRIAHRYDLVNRVLTLRLDV
                     RWRKKLAAMFPDRDNLAVLDVACGTGDVLTIGLKQRKNVSLGVGLDPAARMMARGRDK
                     ARECGLAERIAFVRGDALRLPFADGSFDVVTISFGIRNVTDVPRALAEMHRVLAPGGR
                     VLVLEFSFPDNRALRAAYLVYLRHILPRIGAAISGDAAAYRYLDRTVESFPYGEAFCD
                     LLRRAGFAAVRPRPLTFGVATVYSGEKK"
     gene            25166..26125
                     /locus_tag="PKM94_06135"
     CDS             25166..26125
                     /locus_tag="PKM94_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HOD66257.1"
                     /translation="MKYRRVGRSGLKVSEIALGAWLTYGGPVSASQTAPIVRRAIAHG
                     VNFIDLADIYARGAAEETLGRILKEFRRADLVLSSKLFWPMSDNPNDRGLSRKHIRES
                     IEGTLRRLGTDYLDIYFCHRFDPETEVEETVRAMDDLVRQGKIIYWGTSVWEAAQIER
                     AVGLARDYRAYAPIVEQPRYHLLDRHIEPEIMPVCAHNGMGLTVWSPLAQGLLTGKYN
                     AGIPDGSRGATSMWLKPSLTEANIAAVRRLSELAASLDITVARLALAWVLRRPEISCA
                     IIGATAPEQVDENVKAADTVLSAETLAAIEQILGNDPAARTQA"
     gene            26189..27031
                     /locus_tag="PKM94_06140"
     CDS             26189..27031
                     /locus_tag="PKM94_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOD66258.1"
                     /translation="MRLIFHPTCLTYHLPGHPETPRRVRLAWEHLSRQYETVPPEPAG
                     DEDILTVHTPRHLEMVRTGQFLDWDTPALPFIFDHALLAAGGALTAMRLALDGVAAIS
                     LLRPPGHHAGPDSVAGFCYFNNIAIAAARALEQVDRVAIVDFDCHHGNGTQDIFLGRD
                     RVVYLSLHQSPFYPGTGLFSERNCFNYPLRMGADEEMWLRALDDGLAKVRAFGPDLIA
                     VSAGFDLYKLDPFSFIPLSHEAFRAAGERIGALGRPVFSVLEGGYSDGLGRCAAAYLE
                     GLAG"
     gene            27082..27504
                     /locus_tag="PKM94_06145"
     CDS             27082..27504
                     /locus_tag="PKM94_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOD66259.1"
                     /translation="MKRFEFLDHTADVILQGHGDTLAEAFAAAADGLFAVITDLDRIA
                     PTLDLDVAVEAIDREGLLVAFLSELIIRHEVDRIVAKDFAVVLEGDTRLAARGRGEKF
                     DERRHECGMHVKAVSYHMIEVVAGGPGRPARVRVVLDV"
     gene            27558..29036
                     /locus_tag="PKM94_06150"
     CDS             27558..29036
                     /locus_tag="PKM94_06150"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOD66260.1"
                     /translation="MAWQGPLDKIDAWRYQIPSSFASPVIREKGLRMRVPGLIFTDEA
                     MLEAIRRDDSLFQVANVATLPGIVEKSIAMPDIHHGYGFAIGAVAAFDGAAGVISPGG
                     VGYDINCGVRLMRTDLHLGDVLPRIETLVDTMFANVPSGVGSSGRISLSEREVDEVLA
                     TGARWAVSREYGLAEDLEAMEEGGCLEESDPSVISKNAKARGHDQLGTLGAGNHFLEI
                     QRVDAIYEPDAARVFGLEEVGQIAVMIHTGSRGCGHQICTDYLESMRRASRKYGIALP
                     DQQLMCAPVDSPEARQYFLAMNAAANFAWCNRQVIMHWVRESFEAVFGQPADRLGLRL
                     VYDIAHNMAKREQHDIGGKKRWVYVHRKGATRAFGPGHRDVPEKYRAVGQPVIIPGDM
                     GTASYLLVGTAQAMRETFGSSCHGAGRRLSRTASIKEHRAEDVIRKLREGGIYLRAKS
                     KRVVAEEAPGAYKSVDEVVAISHHAGITRKVARLTPVGVVKG"
     gene            complement(29127..>29385)
                     /locus_tag="PKM94_06155"
     CDS             complement(29127..>29385)
                     /locus_tag="PKM94_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD66261.1"
                     /translation="FELAQNYPNPFNPTTTISFTLPTPGDVTLEVFNVLGQRVAVLAS
                     GPYGAGTHHVEWNASGVSSGVYLYRLSAGEFTETRKMLLVK"
BASE COUNT         4726 a        10347 c         9415 g         4897 t
ORIGIN      
        1 cgcccgaatg cacttttgcg agctggtcgc ccgggcatag ggcagggtgc gctgcccggt
       61 gaacacatcc ttgaactggt tcatcccggc gttggtgaac aggagcgtcg gatcgtcgaa
      121 gggaataacc ggagatgacg ggaccacccg atggtcgtgg cgggcgaaaa agtcaaggaa
      181 agatcggcga atatctgatg tcttaatcgt tgcctccgtc cgttgattca cgcgcgagcg
      241 tctgcacggc ggcgcgggcg atgtcgaaag ggaagccgcg ccgggccagg taattatatg
      301 ctctgcggcg ggccgtttca aggtcaaact gcccgaactc ccgccacttc gcccgcagcg
      361 cgcgctcggc cagctcttcc ggcgagtgct ccgaaagcac ggcgtccgcg gcctcggccg
      421 ccagcgcgcg ctcgatccgc cggctctcga ggaacgcgat cacgtaaccg cgcccgcagg
      481 gttttcggcg cacgaggctg tcggccagct ggtgcgcgta ctgcgcatcg tcgagcacgc
      541 cgcgcgcggt gaaatcgcga atcgccgcgc cgaccagctc cgcatcgaat tgcctcagca
      601 gcttctggcg aagctctccg gccgaatggg ggcgccgcgc cagaaggcgg gtcgcctcgc
      661 tggcgaccgc gaaccgcgcc gcctcggtct ccaactgcgc ggcttgcgat cgggtcagaa
      721 cggttccttt gaccaaccgg tagcggtgca cggtctccgg cgacagcacc agcggccgct
      781 cgtgcgtgga cagatgaatt tcggcccggc tcccgaccag gcggatgtct atcagagaga
      841 ttctctcgtc cattgttgcc aaccccggat caagccttca ttaatagcga tttcttcggc
      901 gggtaaagaa aaaaagcgct gggcgggccg caggctgcgg aaaaatccgc cagcatccgt
      961 gtttttatag tgcattttct cacagacatt tgtacttcat tgttgacagg tgtcgacaga
     1021 acgattattg tgagttgttc tgaatgatcg cgtgtaacct tttataagca agcaggtttc
     1081 cgcgaacaaa atttgtttga accttaaaat tttgctgttc ataaagtaat gactgaggct
     1141 tcgtccgcgc tcaccgagga cggagccggt ggacagccca aacatatcgg ggagcatcaa
     1201 tggatcttga cctgcaggga aatatcgagc ggttcactct tccggaaatc cttcaattaa
     1261 tcgcctcggg ccgcaagtcc gggactctcg ggatccagaa ggctgactcg atcgtcatgg
     1321 tctatttccg ggagggcaac atcatttacg gctacggccc gcgcgaaacc taccacctgg
     1381 ggcagttgct caaggaacgg ggactcatca cggccgagca gctcgaggaa gcggtgcgca
     1441 tgcaggcggt caccgataac agccgccgcc tcggggaaat cctcatcacc cgcggcttca
     1501 tcgaccgcgc cgatctccag gatgtggtcc gccgccaggt cgagagtctc ctctattcgc
     1561 tcctctcgtg gaccagcgga tcgttcaagt tctacgagga tcagttcccg accgaggagg
     1621 agatcacggt ggacctgtcg gtcgagaatg tcattctcga aggactgcgg cggatcgacg
     1681 agatgaacct gatccgggag acgctgccga acctcgaggc cgtctacacg atctcggccg
     1741 cccagggggg gcgcgcccgc aacgtcgccc tgcacgccga cgagtggaac atcatggcgc
     1801 tggtcgacgg gcaccggacg ctcaacgagg tctgccggct ctcgccgctg gggcgcgacg
     1861 agacgctgcg gaaactcgcg caactgaaac tggccgggct gatcacgaag accgaccggc
     1921 cgattgagcc gcccgcgccc gccgccgatc tggagaaaat ggtcggccgt ctggccggtc
     1981 tgctggagag ctacatgacc gagaaggccg ccccccggcc ggccgagcga agaatcaccc
     2041 aaagcctcct ggagcaggtc gattgagcca gattcacacc ctcaagtcgc tcgagcaggt
     2101 gctcgagact ttcctggaac gggtggtcgc gtacaaacag ccccgcctgc aggtgctcga
     2161 ggggatcaac cggctcgatg atctcgcccg gacgccggat cccgggaccg atttcaccga
     2221 gcaggtgggg gagtggtttg cggcccacaa ccgccttctg gaccagaagc agcttcgcac
     2281 cgccgacctc aaccggatcg gcggcatttt ggcggaaatc cgcagccgcc ttgaccttgg
     2341 cggggattcg ccgccggcgg cccacaagat ctcctcggag attgagcgct ggcgctccgg
     2401 cgccccgacc gcggtcccgc cccgcattac gctcgcccgg ggcccggaga ggagttcccc
     2461 gcccgccccg tccccccacc ccccggtgac cgacccgggc gactcgatcg cccgcttcgg
     2521 cgacttcctc acccatgccc aggcgctgta tgccgatacc gccggcaaca aaaaacacct
     2581 cctgtcggtc ctcgacgacc tcctgtcggc cgccaccttg cggcagaaca aagatgcttt
     2641 gattttgtcc gcgttcatca tatattacct gaagctaggc aactataaag tcgagccgtt
     2701 tgttaaacgg ctcaaacatg ccgaggagct ggtgaaggga ggccaaagcc atgctgaggc
     2761 ctgacgagaa agcctattgc caggcgttgc tggctctccg gcagaaagac tatcgggcgg
     2821 cgctgcggtt tttcgatgcc gcggctcccg ggttttccga caaccgggag ttcagcctgt
     2881 accgcgaagc cacccgcctg cttctggccg tcaaagataa attggcggaa ctggagaaca
     2941 aggacactat cgatacgaag gagaccgtga cccatggcca agagacagag cttcgctgac
     3001 aaggccagca agaagtccca ccaggtgatc tgccctgtct gcgaatcccc ggtgcagtac
     3061 gtcaagcacg tgaaggccga gaagaacggg gcgggatgga agttccgcgc ggtgagcgtg
     3121 ggcgtctgca agtgcaacga gaaagaaatc tacgcctgag cggcgcctga accgaatgcg
     3181 caaaccctcc gccgcccggc ggagggtttt ctgttagtcg ttccgcccaa ccgccatgaa
     3241 ccgcccaggc gatactcccg gtcccggaat ttcgtttctg ctggcgaccc tgctcacgct
     3301 ggccgtctgg atgcttgtgg ctctgtttcc cggcgggcgc acctggggac tggccatctg
     3361 ggcctggctg ccgcgcctgc cggccctggt cgtcctcggc ctggccgttc tcgccgcagt
     3421 cgccctgcac gtgttcttgc ggcgcgctcc ggcggccgcc ggcccatcgg acgccccgct
     3481 gtcggtccgc cgccgcccct ggctgctccc ggtggcgctc acaggcgcgg tcgcgctgtt
     3541 ctggtgggcg cgaacccgga cccatttcct cggcgacggc tacacgctgc tcagtctgct
     3601 cgccgccgat cgcccgtttc tcaagacccg ccaactcggg gaggcgctgc tgcacgtgtg
     3661 ggtcaaggac ctctccgggc ttgaggggca gcagggggcc ctgttcagtt tccggttcct
     3721 ttcgattctc tcggggaccg tgtgcgtcgg cgtcgtcgcc tgggcggccg cccggctctt
     3781 tcaggacagg cggagccggg ttctcttttc cgcgggcgtg ctcaccggcg gttacatgct
     3841 cctgtatttc ggttatgtcg agaactactc gctcttcgta acctccgtgt gcgccttcgt
     3901 gctggtcggt ttgctggtcg ctcaggggcg gctttggcgc gggtggctcc tggtcccgct
     3961 gggggcggcg atcttcttcc acatcatggg ggtgacgctc atccccggcg cggtctacct
     4021 gctcctggcc cccacccggc tgggccagcg ggcgcgcggc tggccgcccc ggacctgggc
     4081 ggcgctggcg gtgctcgtcg gcgcggcggg attctccgcc gtggcatact tccgccatac
     4141 gtccgtgttt ttccgtctgg ctgtgctgcc gctctacgac ggtcggctga ccaacggcta
     4201 cacggtcctc tcgcccgccc acttgggcga cttcctcaac ctcattcctt tgctcctgcc
     4261 ggcggcaggg ctgttggcgc tggcgcttgt cggtttcgtg aaatcccgcc ggccggtgac
     4321 ccggccgttc gcttttctgg ttgtggcggt gctttcgacg ctcggcgcgg ccttcatttt
     4381 cgaacccgaa ctcggcatgc cgcgcgactg ggatctgttc gctttcgccg gcgtccctct
     4441 cgtggtcggc ttctactacc tcgtgctcaa tgggcgcagg gaaaggcggc cgacagtgga
     4501 agtcgccgcc ctggggatcc tgctcggcgc ggcggcgctg ggcggccggg tggccaccct
     4561 gcgggatgag caggtgtcgc tgcgcccgat cgcccactac atcgccctcg accccgcccg
     4621 catgtcgccg gtgtttcaca tcctccgcga atactacgcc gcccggggcg atgccctggc
     4681 ggccgattcg gtcgaggccg agtggcggaa gcacaacccc gaggcggagc tgacctcccg
     4741 ggcggtcgac ctgatcgaga tgggccagta tgcggaagca gccgccctcg cccgacgggc
     4801 gatcgagatc aacgccgcgc actggtcggg ctacgccgag ttgggcgccg ctttgatccg
     4861 gatgcagcgc tacgacagcg ccctgagctg ccttcagatg gccggcggac tgaatccgta
     4921 cagcccgagg atcctccaca atctcgcgct ctgctacgat cgcaccggcg actggcggaa
     4981 agccgaacag ctttggcaga aggcgtaccg cctcgatacc acgcaaactc ccacgctcgt
     5041 ggagctctgc cgcctctacc agatccgccg gcttcccgac aagctcctgg ccgctctcga
     5101 acgtctcgcc gaccagccgg acgcgccgcc ggaggggctg gcgaaacttg ccgatatgta
     5161 tgctgaggcc ggccggcttc ccgaggccgg ggaggtattc cggcgggcgg tgcaggccgg
     5221 gctggatacc gcgctggcac ggcagatagt ggccgcccgc ccccgactga aatctttcgt
     5281 cccggggttt ccgtagcccc ggtctgcagg atgaggatgc gccgatggag tggttgcccc
     5341 gtctctggct gctgagtgtc gccgcgctgc tctttcaagc ggcctactac tggccgcgcc
     5401 tgcccgagcg cctggcctcg cacttcgatg cctccggcgt accgaacggc tactcctccc
     5461 gcgcctcttt catagccgtg tggctggccg tggtgctcct ggtcaacatc tggccggcga
     5521 ttatcggccc gctcatccgc aaactcccgc cgggcctgat caacaccccg aggaaagact
     5581 actggctggc gacgcaggag cgccgcaccc gcctgatcgc gatcgtggcc gccatgctct
     5641 caggcatcat gctgggggcc aatgtgatgt tgctcgtcac gttccactac acggccgcct
     5701 acaacaccgg cggccgcggt tcgagcggaa gttggatcgg gtatgccgtc gttgccgaaa
     5761 ccctgctcac cctcggagcc gtggtgtggg ggctggtgac tctcagccgc ccgcccgccg
     5821 acttcagacc gcagcctcct tcgtgagaac catcgagtag aaagccgatt cgtcctcgaa
     5881 caatcgagag acctgccagc ccgtcccggc aacgatctcc cgcaattcct ccggcgacac
     5941 caaaaggtaa tcgaaccagg gagtcgcgag cttctcgtac cggaccctca gccggagctg
     6001 gcctccccta cgcccccgtc ggcgattgca ctcgtggtat tccaggtgct gcacgtccga
     6061 aaccttgtac ggatgtcgca ccgaggcgat gatgcggccg cggtccgatg tcatcccgtg
     6121 aagccgcctc agcattctcc gccccttttg gagcgtttcg agaagtccga aattcccccc
     6181 gaacagcacg acggcgtcga acactcccat gtcggcggag acatcggcga accgcatcag
     6241 ccggacatcc cgcactcccc gctcccggca cacgtgcacg agcagaggcg agtagtcgat
     6301 cgccactacc gggtgcccct tctcctgcag atgcagcgcg acccgtccgg ccccgcatcc
     6361 gacatcaagc acccggccgc gggcatagcg aatcgcttgc cgctcgactt tccaccacct
     6421 ggcgtaacgc tccaggcaca accggtgtcc gacatgactg gcatcgatga acccgtcgtc
     6481 gcgctccaca atgagggagg tgtaccgccc gtgcagatga tcgcacatcc cccgtccgaa
     6541 cgcgtcctct tccggcttca gcatgatccc gctccctgct tcaggtggag ttttacgtaa
     6601 gtcgcaggta cggcacggcg ttcgccgaca tttcccccgc gccttctttg cggtagcgaa
     6661 agtaccccgc cgccgcgatc atcgccgcgt tgtcggtgca cagttccggc ggcggagaga
     6721 acagccgccg cccctccggc gtcagccgct cgcccaggag ggcgcggagc cggctgttgg
     6781 cggccacgcc gccggagacg gtgacgtcgt gtatctggag gtcgcgggcc gcccggagcg
     6841 tcttctccgc cagcacctcg gccaccgctt cctggaacga cgcggcgata tccgccttgt
     6901 gccgcgcgag ctccccgggc gatagcttct tcaggtgcag cgccaccgcg gttttcagcc
     6961 cagagaacga gaagtggtag tcctcccggt cgagcatcgc ccgcggaaac cgcacgtagc
     7021 cggggtttcc ctgctgcgcg agtttgtcca accgcgcgcc ccccgggtag ccgagatcca
     7081 tcagcttggc caccttgtcg aacgcttccc cggcggcgtc atcctgcgtc cggccgagca
     7141 cccggtagac gccgaaccca ttcacctcga tgagcatcgt gtgccctccc gaaaccacca
     7201 gcgtgaggtg gtggggggag aggtcggcgt aggccagcag gtttgccgcg aggtgtccct
     7261 cgaggtggtt caccggcacg aacggcaggc gcgccgcgaa ggccagtcct ttggcgaagg
     7321 tcagccccac caggagcggc ccgaccagcc ccggccccat ggtggcggcg atcagtccga
     7381 ggtcgccgat ccccaccccc gccgtctcca tcgcctgccg gtagatcggc acgatcgttt
     7441 tcaggtgttc ccggctggcg atttccggca ccacgccgcc gaagcgggcg tggtcggcct
     7501 gcgagaggat gacgttggcg agcacccgcg agccgtcggc gatcacggcc gccgcggttt
     7561 cgtcgcacga tgtctcaata cccagcgcga gcatggccgg tcactgcggg cggcgagtcg
     7621 tgaactggac cgactcggcc gaggttttct tgacccggaa attcgagggc gcgtcgacct
     7681 tgatcggcgc cgcgttgagc gagtcgcgcc ggcggtagtc gaccgaggcc acgagcgcgt
     7741 tcgggttgag caggtcgata tcctccggcg gaccggtcag ctccacattg atcacgcggg
     7801 gctgaatcga cacctggcgg tcggccggca cgttgtagac gaccaccggg atgttctcga
     7861 agacccgggt cttgatcgcg agaacctcga cctccaccag caccgaatcg ggatcgagcc
     7921 cgatgcggta tccctccggc cgccggagcg gcaagaggag ggagagattg ttgcgcacgc
     7981 cggtgatctg gcgggggatg gtcgacaccg cgctcaggcg gtcgaccacc agctcggccc
     8041 cccgcaccac cacctggggc ggcagcgggt ccgaaatccg gctcaccgca aaaccctcgt
     8101 cggtcttgat gtcgagatcg agcaccacgt ccaccgtggc ccgcacgatc tcgtcgacgt
     8161 gcaggttgat cgaagtcggc gacaccacgc cgtcaatcgc cacccggttg ccgcctgcga
     8221 tcagcgtcgt gttcgccgcc gacagcggca caacgtgccg ccccgggccg taagcggtcg
     8281 ccaggatgcg cacgccgtag tcccgccagg tctgccggag gagctgtttc cccttcgccg
     8341 ccaccacgac cgtgatcgaa tcgggcggcc ggttggccag cgtcagggag tcatccagcg
     8401 cgatctccga tatcggcacc cgcacctcgt agttgtagat tttctcggtc gcgacatgga
     8461 accacaggag gagccccatg atcagggcca cgatcttcag ccagaagttc tcgagcaggg
     8521 atttcatatg tcacggttcc cacggccggg cggcgggcga ccggccgccg atatggtaac
     8581 acacgtcccg gccgcaaaca accaacaatt gcggaaatcc ggccggaact agtccacgcg
     8641 gtcgggattg ttccagagaa accgggtcag cccccggtcg gtccccaccc acaggtagtc
     8701 cccatccacc gccagtgcgt agacgttgtt cgaggccagc ccgtcgtcga ccgtgaaatc
     8761 ccgcttgaac ggtttgtccg cggtgtgaaa gaggatcgtc agccccttgt ccgaggccac
     8821 cgccgcgatc gtctcgttga ccgccagcgc ccgcgggagc agctgctggg tcatgtcgtg
     8881 gaacgaccgc gactccgccg tctgcagatc cagctccgcc agcccgccgt cggacacaaa
     8941 ccagagcttg tccgcccacc gctcgatgtc gtagacgtgg ctgaacagca cgttctccgg
     9001 atcgcgatac cgctggagtc gctccgcgcc cttcgtcagc cggtaggcgc cgctctccgc
     9061 ggcgacccaa acgtagtcgc cgacttcctc gatgtcccag atgtactggc cctcaaacgt
     9121 cgtggggagg atcagggcgg ccgaatcgga ggccccgatc accaggttca gcccctcgtc
     9181 ggtcccgata aacaccgaat cgccggtcac cgcgatgctc aggacattct cgccggccag
     9241 tccctgccgc tccccgaccg tccgtatgat catttcgctc tccaggtcca tcacgagcag
     9301 cccgcgcgcg gtcccgatgt acaagtgggc ggaatcggag cggagacaat tgatgtcgtc
     9361 ggcgggaaac tccggccgca ccccggactc gaggtggcgg aaagtcatca tcgccggctc
     9421 gaatcctgta atcccggtgc gcaggtcgtc gtacaccgcc ccggacaccc acagccggcc
     9481 gccctcctga tcgagggcgt tgacgcgccg ctggaccagt ccgaacggga gcaggtcgat
     9541 gatgaggttg gtggtccgcg ctttcgccgg gccgtatccc cagatggcca tccagtacat
     9601 cccgctcccg tcgtcgacca tctcgttgat ctgccaggtc cgcccgtagg ggtcgatgag
     9661 ctgtccgccg gcgaaatagt tgaaaccggg cggagcgtgc atgatggccg gcgggggcac
     9721 ctcgcgcgcc gccccgtccc cctcgggcgt ctcgctggtg aggaaccaca tcgccagcgt
     9781 ctggtcgtaa acgtacctcc cgtccggcgt cgccgcatag agcgtgcgcc cgaactggtc
     9841 ggcccagatc cggctgatat cttccggatc gactcccggg atcgccggca ggggctcctc
     9901 ccagcggtcc tccccctggt tgtagcagat gatcccctgg gcggtggcga aatatacgcg
     9961 gacgacggtc acggcgatgc tcctggttcg ggagaaatcc gagaaggtca cggcctgccc
    10021 ctcgggatag ccggcgtggg ccgcaacggc cagcaggagt cccgcgagga tccccgcgat
    10081 cgtcgtgcgc gccttcatgc gagcccgcct gagaggacgt ccaggaccgc cgccgccagc
    10141 agcgcggtgc cgaccggcag ggccgcttcg tcggctatga acgccggcga gtgccagggt
    10201 tggtcggcct tgattttcct gttccccaca cccaaccgca ccatcgcccc cggcgcctgc
    10261 ttgaggtagc aggcgaaatc ctcgccgccg agcatcacct cggtcgtttc gattttaccg
    10321 cgtccgtaga gccgttccat gttgcgggcc agcacacggt tggcgcgcgg gtcgttgctg
    10381 agcaccggat agtcggcgat caggcgcagc tcgacccggg cgccgcgggc ccggccgatg
    10441 ttttcggcca cccggcggat caggcgcggc accgccttct cggcccgcgg cgacagcgtc
    10501 cgcgccgtcc cggccacccg cacctcgtcg gcgaccacgt tgcgcgccac cccgccctcg
    10561 atctgcccga acgtgatcac ggccggctcg accgggtcga tctcgcgcgc gaccactttc
    10621 tgcaccgcct cgatcacctc ggcggcggtt gtgatcgcat ccactcccag gtggggccgg
    10681 gcggcatggg cgctccggcc gcgcacaatg agatcgaaat cggtcaccgc cgccatcgtc
    10741 ggaccgtccc gcaggccgat tttccccgtc cgcacgtgcg ggtcgacatg gagaccgaac
    10801 accgcggagg cgtctgtcag ggcgcccgac tcgatcatcg gccgcgcccc gccggggggc
    10861 atttcttcgg agggttggaa gatgaaccgg acggttccgg cgatttggcc gcgcagaccg
    10921 ctcagcacgg ctgccactcc ccagactacc gccatgtgca tatcgtggcc gcaggcgtgc
    10981 atgcagcccg ggtttttcga tgcgaacggc aggccggtgc gctccgtaat cggcagcgcg
    11041 tcgatgtcgc tgcgcacgac caccgtccgc ccccccgcaa ccccctgcaa ctccgccagc
    11101 acaccggtgt cggtcgtggt cgcaagcaac cgcaggccca gccgccgcag cgcggcggcc
    11161 aagtactcgc tcgtccgaaa ctccctcgcg gaaagttcgg gatactgatg gaggtggcgg
    11221 cgcagcgccg cctggcgggc agcgatccgc ttcgccgcgg cgagaatccg gtctggtcgg
    11281 tcaggggagt ggggcacgtt caggttcgct ccggtggggg tgatccggcc ttacggccgg
    11341 ctgttccgtt ttcctgatag cgcttgtact tattggcaaa cacatgcgcc agccgcaaag
    11401 gttccggcaa ccggtagtcc cgcagacagc ggaccaccag ttcccgggcg ccggccagat
    11461 cgcaaccgtg tccgggggag acaaagatcg gtttcacccg gtctttcgaa cggtaggcgc
    11521 acccgacttc ggctccctca atgcggatcg gctcccaact ccctttcgca tcggctaccg
    11581 gccgatgagt tcccgcgagg agcttgcggc tgcacccggt cgagggccgg ttgaacagga
    11641 cgccgagcag gccggccatg ccgcagcggg ccgggtgggc caagccgtgg ccatggatca
    11701 tgagcatgtc cggctcggtc gccaggcggt ggagcgctgc gatgaccgcc ggtccctcgc
    11761 ggaaaaacag cagccccggc acatagggaa aaatcgcggg ggcacacccc tcggcccgat
    11821 ccaccggctc caggccggga taccggacga cgacggcggc ggcgtaaatc cgcttgccgt
    11881 cgcggccata ggcggtgtcg accgccgcga tcaggcgggg ctcggaggcc gcgggggcaa
    11941 tccggatctc gcgggcaagg gccgtctgga gcgccgccgc ctcggcccgc gtcaccggcc
    12001 acttctcaat ctgccgatac tgcacgcctg cctgtgaaca ccgcgggctg cggttctgaa
    12061 ttctgcgaaa cgatacgaag aacctcccgt cccgtcaagg cgcgggaccg tcactgcgaa
    12121 ccccgcaggc ggaagaaccc ccactgcggg agatcgtcga caagtgccgc gaggtgcccc
    12181 ggcgtgatcc agggggagtg gcggtccggc gtcagcagcg gaatgtgcag gtcgcaggcg
    12241 ttcggtttgg ggcagccggt cgccaccgta tacatccgct ccccctcgtc gttgaccagc
    12301 atgttgacca caatgtagac ctttccgacc aacaggcggt cgtcgtgccc gatgacgagg
    12361 tctccgggtc gcacctcctc aggggggagg gaatcgcagt tggccgccag cgtctggtac
    12421 gtctgctgct ccatcgccac cgtcaggaac cggtagaatt ccatctcgct ggcctcgcgc
    12481 ggcgtggccg gcaccagccg cggcccgccg agcggcgtgt agctcacttt gctctggagc
    12541 caggcggcgt aggcgagcgt gtcgccgtcc gcccgcgtca cgatgtgcat ggccgactcg
    12601 cggccggcgt accggcagta ctccgcgaac acgcgcagcg gaaacccgat atcggtgtac
    12661 atcgtccccc gccagggcat gtgcacgacg cgcgagacct cgccgtactc gtacgctttc
    12721 ccgcccttcc acaccccgac cggcttccac tggttccaca gcggcaggtg ggagacccag
    12781 tactggtatc cggtcagggc ggccgaatcc ggccggtgga acccctcggg gagacggaac
    12841 tccgtctcga acgtgtagat cgtgtcgtcc atgctgatga aggagtaggt ctggcggtac
    12901 caggccagca gggaatcaaa gggggccggc ggggccggat ccgccccgcg gctgtcccgg
    12961 agcgggacgg ccgccgccag aatcgcaagc agcgcagcga cgcagcgcgt cattcctctt
    13021 tcatctcctt ccacagccgg gccgcccggc gcaggtgggg aatggtgatg gagccgccga
    13081 ccaccagcgc gatcgccatc acctcgtcga actccgcgtc ggtcacgcgg ttctcccggg
    13141 cgcggatgac gtggtaggcg atgcagtcgt cgcagcggag caccagcgag gccgccagtc
    13201 cgagcagctc cttggttttg gcgtccagcg caccgtcccg gtacacggcg ccgtccagag
    13261 cgaagaagcg cttgatatcg aggttgtccc gctcgaggac aatctcgttc agcttctccc
    13321 gctcctgcct gaactgttgt acccgatccg acatcgattt ctccgatatt tttccgcgcc
    13381 gccggggcgg ccgttgccgc cgccccgggg accgccgccc cttatttgcc gctctcggcc
    13441 agcagcgcct ccatctggtc caccaggtcg gcgaacagct tcatcgtccg ctggaccggt
    13501 tcgggcgagg tcaggtcgac ccccgccttc ttcaggatct ccaccgggta gtcggaattg
    13561 ccgaccgcca ggaactgcat gtaggtatcg agatatttcg gatcctcgag caccttctgg
    13621 gacatcgcct gcgccgccgc gtaggaggtg gcgtactggt agacgtagta ctggctgtag
    13681 aaatgcccga tgcgcaggca ccccaggtcg ttgaccgccc cgatcgtcag gtccggcccc
    13741 cagtacttct ggtagatgtc gcggtaggtc ttgcgcatga attcggccga cagcgcctgc
    13801 ccgctctcga tccgctcgtg gatcgtcagc tcgaattcgc tgaacatgac ctgggtgaag
    13861 aacgacccga tgatctgctc gatgtagtag ttgagcagct ccatcttgtc gccccggtcg
    13921 gtcgtgttgt ccaggaggta cttcatgaga accgcttcgt tccccgtgga ggccacctcg
    13981 gccgcgaaga gcgaatggtt gtggctgatg tacggttcgt gccggttggt gtagtagctg
    14041 tgcatggcgt ggcccatttc gtgggcgagc gtgaacaccg aggccagcgt cccgttgtaa
    14101 ttcagcagca cgtaggggtg cgagctgtaa gttccccacg agtacgcccc cgaccccttc
    14161 ccctccgtct cgtagacgtc gatccagccc gacgtgaacc ctttctcgag gtcagtaagg
    14221 aactgcggcc ccagcggctg caagccgcgg cggaggatct cctgggcctg ctcgtaggta
    14281 tactccttcc gcccctccgg catgagcggc acggagaggt caaacgtgaa gagcgtgtcg
    14341 accccgagga tgcgtttgcg cagtgaggcc cacttgtgca gcggggcgag gttctcgttg
    14401 accgccgcga tgaggctgtg gaagaccgcg accgggatgt tgtagcggtc gagcgaggac
    14461 tccaggcagg tcgcgtagcc gcgcgcctgg gcctggaagt agtcgctctt gaccgaggcc
    14521 gccaacgtcg ccgccagcgt gttctcatag tcgaggtagg cggcgttgta ccggtcgctc
    14581 acccgtcggc ggagttcgcg gtcggtggac tcgagcatct tgtatacgcg ctctttcgtg
    14641 agcgccacct ccgcgccgtt ctcatcggcc accgtgccgt agtcgatatc ggcgttgtcg
    14701 accatgttga agatttctcc ggatgcgctc accgcaggcc cggccagcgc caggagagcc
    14761 tcctccttgt ccgacagcac gtgcgcctgc ttgcggaaga gatcgtccag gtagtggcgg
    14821 tacaccgcca gcttcgggtc ctgcccggcg aatccgacga tcttgtcctt gccggcggcg
    14881 atgatctccg gttcgatgaa ggaggtcccc tcggaaatcc gggaccacaa ctgccggatg
    14941 cgccctccca tctcctggta ggtgctcacc cggttgtcct catcgagtat caggtaggcg
    15001 tagacataca ggttgtcggc gatcagctcg atgctgtcgc gggtggccag acaggccagg
    15061 agggtcgccg gtccctcgcc cagacggccg cggtattggt cgaacagccc gatgcttccc
    15121 tggagaccgg cgaaggcggc ctcccaggcg ctgtcggtcg ggaagatgtc ggcgacccgc
    15181 cacttgtatt ggtcggcgat atccgcccgc tggggaatgg cgctgtcggc ggaaatgacg
    15241 gcggacgcgg ctgcgagaaa cagcccgacc gcgaggagga cgggggaaag gacacgcgat
    15301 gtgagccaca taggaggtct ccgggatgag tatttcactt gttccacgct tgctttcggc
    15361 gttaccatag gctgcaaata tacgcgccgc ccgcgcgtcg cgcacgaact tttcttgaag
    15421 gtcaccgcca tgtacaccta tcggactgcg gtcaagctcc acgataccga cgccgccgga
    15481 gtgctctttt tcgccaatta tttccggctc gcccacgagg cctatgaggc ctggatgtcc
    15541 gccgtcgggt tcgacttctt ccacctgatc cacgaggccg actatctcgt gctcatcgtc
    15601 cgcgccgagt gcgatttcag gcgcccgctc cgcgtgggcg acggtgtgac cgtgcggatg
    15661 accgcttccg gcatcggccg ctcctccttc acgctcgcct acactgttgt ggacgacgac
    15721 gaccgcgtcg tcgcccaggg cgccaccgtg catgcctgcg tgggcaagcc ggatctcaac
    15781 tccgtccctc tcccggccgg actgaaaacg gaactggaga agctgcagta aggggggcgg
    15841 ggcgccggca acccttcggg gagatcctga agcttccgga ggagcgttct tttcgcggcc
    15901 ctctctcccg taaaagggaa cgctcgccgt ctaccccgcg gtcttctctc ctgcctgccc
    15961 gtagagtgct tgcgccaccc gctcgagata ctttcggtcc ggtttcaatc ccgcccgcgc
    16021 ctcctcgggg atcaacgcca cccgcaccgg caccgcgtag gccgggagcc tctccctgaa
    16081 catctcccgc acctgcgcgg gagtatccgc ccgcccgccg gcgagaagga cataatcccg
    16141 caggagccgc gtcgtgccgt cgtacgtgcg cggatctttc cgcacgaaag agacgaaagc
    16201 gaacggccgc tgcccgtact gcgcgtcctc gaccgcgacc accaccgcct cggcgacatc
    16261 cggcaactcc agaagcatct tttccaccag ctccggctgc acgttctcgc cgccggagat
    16321 gagcatgtgg tcctttcggc cggtcacgcg aagatacccg tcgggctcca gctccccccg
    16381 gtcgcccgtg tggaaccatc cctcggcgtc ggcgatcgtc cgcaggccat tcgattccag
    16441 gtaccccggc gagagcacag gcccccgcac gaggatctcg cccgactcat cgattcgaag
    16501 ttcccggaag ggcaggagtc tgcccgaccc gaacagccgc cggaggggat cgtccggggc
    16561 cgtcgtggtc acctgagagc acatttccgt cagaccgtag gaggtgaaca gcggcagacc
    16621 ccgcgcggcg gccgcttcga tcaggccgcg cggcgccgcc gctcccccga tcaggacagc
    16681 cttcaacccg gcccggtggc ctccctcggc gaggtaccgc cgcagttgcg tcggcaccat
    16741 cgagcagtgg gtgacgccga gcgtctcgat cgactccgcc ggattcgccg gtcggcgccc
    16801 cggcacgacc gccgcgccgg cgagcatcgc ccggtacagg atcgcgatcc cgcccacgtg
    16861 gtgcagcggc agcgacaaca gccaccggtc gccctccgcc agcgggaggt tctcgtttga
    16921 tccgagcgcg ctgtagtagt ggttcccgac cgtgtgcaga cacaacttcg gcgtcccggt
    16981 ggaccccgag gtgagcacgc cggtcgcctc caaacggagg ttgcggcgga aatccgtccg
    17041 gtccgcttcc gccggcgaga ggagaatctg cgcctcaatt tcggcaacct cctcccaggt
    17101 ggcggcggcg aaggggatat gctcccggcg gtcctgagcg acgaccgcga caattccctg
    17161 ctccgccggc agcgccgccc gcaccagcgg cgccggcaga gcgccgtcga ccaccagcgc
    17221 ggtcgccccg gctcttcccg cggccaggaa cgtgatgatg acgtcaaggg agggctcgtc
    17281 cccggctttc gggacaagca gagcgagccg ccgcagcggt ccgagccgcg tttccaagcg
    17341 ggccagcgcc gccgcgcggc gattcacggc gcggttggcc tcggcgtagc tgtactcccg
    17401 cgccggcgtc acgagaaagg gcgccgcggg ccgccggcgc gccgcctccc ccagcggaca
    17461 ggggatatca gtcatgcgcc ggttctccga ggcggtccgg acgcacttcg aaagtccccg
    17521 cgggaagggc ggacaggtcg atttgcccct gcgcgatgcg gagcggttcc acggtcagat
    17581 cctcggcgag cgcgtcctgc gtgtccagtc ccatggcgac attctcgccg gcctcgccgt
    17641 cgttgaggca ggcgccgatt tcggccagca tcgccagtgt gaggctcgat tcgtaggccg
    17701 agctgatcac cgccagcatc cccgcccggc gcgcccgccg ggcaaaccac atcgcccact
    17761 cgaatcccag cagggtcggc ttgagcacga tcgcccgcac cccgcggact tgcgacagcg
    17821 ctttcggcga gatcgtgcgc aggctctcgt cgagcgcgac aggaaggcgg aatccgccgc
    17881 ggtgagtcag ttcgagcagc tcgagaaaac tgcgcaccgg ctcctcgatg tactcgatgt
    17941 cgtacggcgc ggccgcctgc ccgaaccgga tcgcggtggg gatatcgaac gcccggttgg
    18001 catcgagccg gagcttggcg cgcgggccga tcgtctcgcg cacccggcgc accgcctcta
    18061 tgtcctcgtc gaacggccgc cgccccactt tgagcttgaa cgccccgaaa ccttcgccct
    18121 tgcggcgctc tgcctgcccg aacgcctcat ccgccgatcc gctcagaagg gcgttgacgg
    18181 tgatcctctc ccggggattg ggacagagca ggcggtgcag cgatttcccc tggcggtgtg
    18241 ccaccagttg gagcacggcg gtctccatcg cgaccgacac cgaggctgtc aaccagctgt
    18301 ggcccagcca gttgccgaaa ccgccatcga gccgctcgag accggcgggg atttgcgccc
    18361 cctgcacacc ggcccggagt tcccgcagtt cccgctcggc atcggccagc gattcccggc
    18421 tgtgtcccgg aagcggcgcc gcctcccccc aggccgtggc gccgtcctcg agcgtcactt
    18481 tcacaagcat gccctcgcgg gagcgcagtt cctcgccccg aatgaccggc ggccgccgga
    18541 acggcagtac atagcggtag agctgaacct gcgcgattct caccaccacc atcctatcga
    18601 aaacagcagg ctgtacagca agagcagttt ccccgtcgtg gccagcagcc tgttgagcgt
    18661 cggtccgtcg gtggcgcggc ggatcgtacg gaacgccgcc agcgccggaa gtatcgtgag
    18721 cgatgcaata atcgccgacg agtgcccctc ggtcaacagc gcgagcgcag cgggaatgcc
    18781 cgcggcgatt gcgacggcga ggacatactc ccactgggcg aaggttcgcc cgaaccgcgc
    18841 ggcgagcgtg cgcttgccgg cccgacggtc gctctcgatg tcgcggagat tgttgaccgt
    18901 caggatcgcc acggagaaca acccggtccc cgccccggcg accagcaccg ggggagtgat
    18961 ggccagcgtc tgcacgtaat aggtcccgcc gaccgccacg ggtccgaagt agacgaacac
    19021 aaagaggtcg gccagaccga tatagccgag cgggtacggg ccggccgtgt agagaatccc
    19081 ccagaggacg gagctgagtc cgatgacggt caccggccag ccaccgcgat atacgaggta
    19141 ggcgccgcag agcacggcca gcccgaacac cagcgccgtg gccgccttca tcgcgcccgg
    19201 tgtgaccaga cccgcctgag tcacccggat gggaccgacc cgggcgatcg catcggcccc
    19261 cgatttgaaa tcggaataat cgttggcgaa attggtcccg atctggatga gcagcgcccc
    19321 ggccagcgcc gcgagcgccg accagaggtg aaaggcgccc gcctccgccg ccatcgccgt
    19381 acccatcacc accggcgccg cggaggcaaa gagcgtcttg ggccgggccg cgagcagcca
    19441 cacctgcggg cggccgatcg gcgcgcccgc cgcgactgtg tcagggctgc cggggaaact
    19501 tgccgaaatc cggtttgcgc ttctccttga aagcatcgcg tccttcctgc gcctccgccg
    19561 tcatgtagaa cagcatcgtc gcgttgccgg ccaactcctg cagccccgcc tgcccgtcgc
    19621 agtcggcgtt gagcgccgct ttcagacagc ggatcgcggt gggcgagttg gcgagaatct
    19681 cccggcacca ctggaccgtc tcctcctcca gccgggcgag cgggaccacg gcgttgacga
    19741 gccccatctc cagcgcctgc cgggcgtcgt accggcggca gaggaaccag atctcccgcg
    19801 ccttcttctg gccgaccagg cgggcgaggt aggatgatcc atagccgccg tcgaacgacc
    19861 ccactttcgg cccggtctgt ccgaagacgg cattgtcggc cgcgatcgtg aggtcgcaca
    19921 gcacgtggag cacgtgcccg ccgccgatcg cgtagccggc caccatggcg accaccggct
    19981 tggggcaggt ccggatttgc cgctgaaact ccagcacgtt caggcggttg accccgctgg
    20041 tatcggtgta gccggagtcc ccccgaatcc tctgatcccc ccccgaacag aacgcctggt
    20101 cgcccgcgcc ggtgagaatg atgacgccga tctgcgggtc ctcccgcgcg tcggccagcg
    20161 ccgccatcat ctcctgcacc gtcagcggcc gaaacgcgtt gcggacctcg gggcggttga
    20221 tcgtgatctt ggcgatcccc tccgccttgt gatacgtgat atcgctgtat gcgcccgctt
    20281 ctgtccagat gatcctcgcc atggcaccct ttctatccgc tcaggaactc ctgtaccagc
    20341 tcgacaaacc gggccggttg ttcgacgtgg accgcgtgcc ccgcccccgc caccgtcgcg
    20401 acccgcgccc ccggcatccg ctcggccatc cgcgccgcga tctcccggaa tttagcatct
    20461 tcggcgccga caatcaacag cgtcggcggc gcgtcggcca ggcggtccca ctgcggttcc
    20521 tgcgccccgg tccccatctc acgcagcgcc cgggccagag ctccgccgtc ctgggcgcgg
    20581 cggcgttcca acatcccggc gaattcctcc ggaacccgct taagtcccgc gaacatcggc
    20641 tgatcatacc agtcgcggag aaagcgctcg aggggctcgg tctccagccg ccgcgcgagt
    20701 gtatcgtcct gccgccgccg cgcgatccgc tcgtctccgt cgctcagtcc cggcgaggcc
    20761 gactcgagca cgagccgccg cacgcgcgcg cggcagtgca gcgcgaggtg cagccccaac
    20821 cgccccccca tcgagtagcc gatcagatcc gtctgcgcga tcccccggcg cgccagctcg
    20881 agggcgagcg cggcggcgac ctgctccatg cgccacgatc ccgcgccctc cggaccccgg
    20941 gtctttccgt gccccgggag atcgggagcg aggccatacc gttctccggc cagggccgcc
    21001 atgacggtgc tccagtccgc gcccgttccc atgaaaccgt ggagcgccac caccgccgga
    21061 ttgcgcggcg accccgccgt ctcgaggtga aaccggaaat cccccgccat gcgcttaccc
    21121 ctcgagcgcg cggaccaccg cctcggtgag ccgggcgtgc agttgccggt tgcgccgccg
    21181 gtctgttcgc acctcgatga gcgtcccccc gcccggcccg gcctgggcct cccggtagat
    21241 gcgcgcgaac tcttcggcgc tcgtgggatg ggcgtacgaa agcccgaaca tctgcgccgc
    21301 ttgcttgaac gtgatcccgt ggggcgttcc ccagtacggc tcaaacaaat cggcgcgctc
    21361 ggcgatcggg aggaaatgga atattccgcc gccgttgttg ttcagcacga cgatagtcat
    21421 gggctgcgcc aagcgccgca cgagcgccag ggagttgagg tcgtgcaaca gcgccagatc
    21481 cccggtcagc agggtcaccg gccgctccca cccgagcccg aacccggccg ccgaggcgac
    21541 cgtgccgtcg attccactcg cgccccggtt ggctgcaacc ggcgccgccg ggccgtcgcc
    21601 ggcggccagg gcatccaggt cccggatcgg catgctcgag gcgacaaaga gcgcgcccgc
    21661 ggctgagata ttctccgcca ccagccgcgc cgtcagcggc tccgacagcg tcgcatcctc
    21721 cgcaagcact cgggcgatcg ccgcttccgc cttgtccgca cccgcccgcc aggccgcaag
    21781 gccggtctct ttggctttct ccctcaggaa cggcaggaga aaactgcaga acatgtcgat
    21841 gtccgcttcg aaccggtggg tgacctgatg gtgcgggtcg tggcgttcgg gggcggcggc
    21901 tgcgtggata taggccgccg gttgcgaccg tttcagccat tgggcgaccc gcttcgaggt
    21961 tacgatcgga ccgaagtgga gcaccgtgtc cggcgcgaga gcgtcggcaa acgattccgc
    22021 ggcgagcatg tactcggccg gtgcgatcac gtgtcccgcc gccccgagcc gcaggcccga
    22081 cccgatatcg gctatgaccg gccacccgag cgcctcggcc actttcgcga tcgcgacccg
    22141 ctcggcctcg cgccgaagac gcccgaccac gatcacaccg cggcgcgccc ggttcagcag
    22201 cccggtcgcc gcttccatcc gcgccggttc gcacactttc accggcgagc gatagattgt
    22261 ccacgggctc tcgcccgttc gccagcaccc gaccgaccgc aggtagtcgc cgaaatcttc
    22321 ctccgcgccg accggggcga gcggttcccg gaacatccag ttgaggtgca ccggaccggc
    22381 cggtccgtgc tgcgcccggt agaccgcctg gtcgatcgtc gtgagcacaa actccggcgg
    22441 tgtctcccgg ctggggcagg ggagatcgaa aaaccaccgg ggataatcgg cgtagagctt
    22501 cgtctggtcg atcgtctgat tcgcgccgct tgcccgcagc tcgggcgggc ggtccgccgt
    22561 gaggaccagc agcggcacgc cgtccatcgc cgcctcgatc accgccgggt agaggttggc
    22621 tgccgcggtt cccgaggtca cgaccacggc cgccggtttt tccgtcgccc gcgcgtagcc
    22681 gagcgcgcag aaggccgccc cgcgctcgtc gaaatggacg atcttcctag ccgcctcgtg
    22741 ccgcgccgcc gccgccgtca gcggcgtcga ccgcgagccg gggcagaggc agaacaacct
    22801 caccccgcag cgcaccagtt cttcgaccgc ggcctccgcc cagaggctgt tcagattcgg
    22861 aaatcggctg ttcatggccc cgccagcagc ttcaggtagt tatcgatctt attttcgatc
    22921 tcgtcccatt ctctgtcggg atgcgaaccg ccgacaatcc cggctcccga atagagccgc
    22981 agcgtgtctc cggccgccag cgccgagcgg atcgcgaccg cgaattccgc gctgtctgcc
    23041 ccgatccaac cgaccggtcc ggcataccag ccccggtcga aagcctcgag ttcgcgaatc
    23101 cgctgccggg cggttgcggt cggcgccccc ccgaccgccg gcgtcgggtg gaggcgcgcc
    23161 agaatatccg cgtccccgac gccgtctctg agaacgcccg cgaaacgggt cgcgaggtgc
    23221 tgcacgcgcg ccagtttgac cagttcccgt cccttctcct cggctgcctc ccggcacagc
    23281 gccgcgagcg ccccgcgcac cgcgtcggcg acgcaggcgt gttcctgcgc ttctttccgg
    23341 ctggtcagga gtttctcgcc caagagggcg tcctccgccg gcgtctcccc gcgccgccgg
    23401 gttccggcca gagcctcgct cgtgatccgc cgcccctccc ggcggtacag ccgctccggc
    23461 gtcgctccca ggaaggcggc gccgtccggc ggcttgaagg cgaagacgaa acagcccggc
    23521 gccgaggccg ccagccgggc cgtcaggcgg aacggatccg ccggactgcc cagctcacag
    23581 cggctttcgc gcgcgagcac gatcttgtcc atccgcccgg cgttgatttc gtccaccgcc
    23641 gctccgacaa tgtccagcca atcctctcgg cccggcgtat cgacccgccg gcgcacgccg
    23701 gccggcggga taccggggtc cgccggcgcg aagcgcaact gcgccaggtc ttcgatgatg
    23761 cgggaaacct gcccgcggtc ctcggggaac cgcagattga ctgccagcac ggccgccccg
    23821 ccgcgggcgg cgagctcgaa gcgggggagc acaaaccgcg ctccgccgaa ctgccgccag
    23881 tgatcggcct gctcccggtc gctgtcgaac cgtattccgc cgtagcaccg cacgccctcg
    23941 gcgtcgccga tcagttcccg gatccgcccc atcaggtcat gcggatcggc gcagaccgcg
    24001 cggaattcca ccgccgcggc gcccaccccg gcgacggccg agcggtcctc gcggtcgctc
    24061 cagaagatcc gctcgctctg ccgctgcgcc gcgagccagg ccgccggttc catcgcggcg
    24121 agagggacct cgcaccgctc cacgtgcggg ctgcccgccc gcggatgcgc cgcccgccgg
    24181 gcgatctctt cggccagatg ggccgccgcc ccggcgggcg tgtgcgtgtc gctgtctttg
    24241 aatactgagg gcatcatggg cctgcaccgt tggttccgca ctatatcact ttttctcccc
    24301 gctgtaaaca gtggccactc cgaacgtcag cggccgcggc cggaccgccg cgaacccggc
    24361 ccggcgaagg aggtcgcaga acgcctcgcc gtagggaaac gactccactg tccggtcgag
    24421 gtagcggtag gccgccgcat ccccgctgat cgccgcgccg atgcgcggga ggatgtgccg
    24481 gaggtagacc aggtaggccg cccggagggc gcggttgtcg gggaaggaaa actcgagcac
    24541 cagcacgcgc ccgccggggg ccagcacccg gtgcatctcg gccagtgccc gcggcacgtc
    24601 ggtcacattg cgaatcccga aactgatcgt cacgacgtca aaacttccgt cggcaaacgg
    24661 caggcgcagg gcatcgcccc gcacgaatgc gatccgctcg gccagcccgc attcccgcgc
    24721 tttgtcccgg ccgcgggcca tcatgcgcgc cgccggatcg agccccaccc ccaggctcac
    24781 attcttccgc tgcttcagcc cgatcgtcag tacatccccg gtgccgcagg cgacgtcgag
    24841 cacggcaaga ttgtcccggt cgggaaacat cgccgccagc ttcttccgcc agcggacatc
    24901 caggcggagt gtcagcacgc ggttgaccag gtcgtaccgg tgcgcgatcc ggtcaaacat
    24961 ctggggcgac tccccctttt cggggatcgg gtccgccgtc ttgtccgcgt gatcttccgc
    25021 cgccatttct atccgctttc cggccgatcc gttccgaccg ccgcaatata cgcaatccct
    25081 ccgctccctc aaacccgatt gcctcgcgcg gtcctttttc gtaccttgcc caaaaggctg
    25141 ccgcaacgcg aaaggatcag cgttcatgaa gtatcgtcga gtcggccgca gcgggctgaa
    25201 agtctcggag atcgctcttg gggcctggtt gacttacggc gggccggtct ccgcctcaca
    25261 gactgcgccc atcgtccgcc gggcgatcgc ccacggcgtc aatttcatcg acctcgccga
    25321 catctacgcc cggggcgcgg ccgaagaaac cctcggccgt attctcaagg aattccgccg
    25381 cgccgacctg gtcctgtcca gcaagctctt ctggccgatg tccgacaacc ccaacgaccg
    25441 gggcctctcg cgcaaacaca tcagggaatc catcgaggga acgctccgcc gtctcggcac
    25501 cgactacctc gacatctact tctgccaccg cttcgatccg gagacggagg tcgaggaaac
    25561 cgtccgcgcc atggatgacc tggtgcggca ggggaagatc atctactggg gaacctcggt
    25621 gtgggaggcg gcccagatcg agcgggcggt cggtctggcc cgcgactacc gcgcctacgc
    25681 gccgatcgtg gaacagcccc gctaccatct gctcgaccgt cacatcgagc cggagatcat
    25741 gccggtgtgc gcccacaacg gcatgggcct gaccgtgtgg tcccccctcg cccaggggct
    25801 gctcaccggc aagtacaacg cggggatacc ggacggaagc cgcggcgcca cgagcatgtg
    25861 gctcaagccg agcctgaccg aggccaacat cgccgccgtc cggcggctca gcgaactcgc
    25921 cgcgtcgctg gacatcaccg tggcccggct cgccctcgcc tgggttctgc gccggccgga
    25981 aatcagttgc gccattatcg gcgccaccgc gccggaacag gtcgatgaga atgtgaaagc
    26041 ggccgacacc gtcttgtcgg ccgagaccct cgcggcgatt gaacagatcc tcggcaacga
    26101 cccggcggcc cgcacccagg cgtaacatcc ccctccggtc cgagagggtg cggggtccgg
    26161 gcgcgcggcc gcgaacggat tggcacagat gcggctgata ttccatccga cctgtctgac
    26221 ctaccacctg ccggggcatc ccgaaacgcc ccggcgcgtg cggctcgcct gggagcacct
    26281 gtcccggcag tacgagacgg tgccgccgga accggccggc gacgaggata ttttgaccgt
    26341 ccacacgccc cgccacctgg agatggtgcg gaccgggcaa tttctcgact gggacacgcc
    26401 cgccctcccg ttcatcttcg accatgccct gctcgcggcg ggcggcgccc tgacagcgat
    26461 gcggcttgcg ctcgacgggg tcgcggcgat ctccctcctc cgtccgcccg gccaccacgc
    26521 cggcccggac tcggtcgccg ggttctgcta tttcaacaac atcgccatcg ccgccgcccg
    26581 ggccctcgaa caggtggacc gcgtcgccat cgtcgatttt gactgccacc acggcaacgg
    26641 cacgcaggac atttttctcg gccgcgaccg ggtcgtgtac ctctccctcc accagagccc
    26701 gttctacccc ggcaccgggc tgttttccga gcggaactgc ttcaactatc cgctccggat
    26761 gggcgccgac gaggagatgt ggctgcgggc gctggatgac ggcctggcga aagtgcgggc
    26821 tttcggcccc gacctgatcg cggtgtcggc cgggtttgac ctctacaagc tcgatccgtt
    26881 ttcgtttatc ccgctgtcgc acgaagcgtt ccgggccgcc ggggagcgta tcggcgcgct
    26941 cggccgaccg gtgttcagcg tcctggaggg gggctactcc gacggcctcg gccggtgcgc
    27001 cgccgcttat cttgaagggc tggccgggta aaccggccgc aattgtcctt gcggccgccg
    27061 ccgcccggca tactatacgg tatgaagcgg ttcgaatttc tcgaccacac cgccgacgtc
    27121 atcctccaag gccacggcga cacgctcgcg gaggcattcg ccgccgcggc cgacggattg
    27181 ttcgcggtga tcaccgatct cgaccggatc gcaccgacac tcgacctcga cgtcgcggtc
    27241 gaggcgatcg atcgcgaggg gctgctggtg gctttcctca gcgagctcat catccggcac
    27301 gaggtcgacc ggattgtggc gaaggatttc gcggtcgtgt tggaggggga cacccgcctc
    27361 gccgcgcgcg ggcgggggga gaagttcgac gagcgccgcc acgagtgcgg gatgcacgtg
    27421 aaggcggtgt cgtaccatat gatcgaagtc gtcgcgggcg gcccgggccg gccggcccgg
    27481 gtgcgggtcg tcctcgacgt gtgaggatcc ccgcggccgg gaaatgcgcc cggcgccgcc
    27541 ggaaatcgga gtgtgctatg gcatggcagg gtccgctgga caaaatcgat gcgtggcgtt
    27601 accagatccc gtcgtcgttc gccagccccg tgatcaggga gaaggggctg cggatgcgcg
    27661 tgccggggct gattttcacc gatgaggcga tgctcgaggc gatccgccgc gacgactcgc
    27721 tcttccaggt cgccaacgtc gccaccctcc cgggcatcgt cgagaagtcg atcgccatgc
    27781 ccgacatcca ccacggttac gggttcgcca tcggggcggt cgcggcgttc gacggcgcgg
    27841 ccggggtgat ctccccgggc ggggtcgggt acgacatcaa ctgcggcgtg cgcctcatgc
    27901 gcaccgacct ccatcttggc gacgtcctcc cgcggatcga gacgctggtc gacacgatgt
    27961 tcgccaacgt cccctccggc gtgggttcct ccggaagaat cagcctgtcg gagcgcgagg
    28021 tcgatgaggt gctggccacc ggcgcccggt gggcggtcag ccgcgagtac ggcctcgctg
    28081 aggatctcga agcgatggag gaaggggggt gtctggagga gagcgacccg tcggtgatct
    28141 cgaagaacgc caaggcgcgc ggccacgacc aactcggcac tctcggcgcc gggaatcact
    28201 ttctcgaaat ccagcgcgtc gacgccatct acgaaccgga cgccgcgcgg gtgttcggcc
    28261 tggaggaagt ggggcagatc gcggtcatga tccacaccgg gtcgcgcggc tgcggacacc
    28321 agatttgcac cgactatctg gagagcatgc gccgggcctc aaggaaatac ggcatcgcgc
    28381 tgcccgacca gcagctcatg tgtgcaccgg tcgattcccc cgaggcccgg cagtattttc
    28441 tggcgatgaa cgcggcggcc aacttcgcct ggtgcaaccg ccaggtgatc atgcactggg
    28501 tgcgggaatc gttcgaggcg gttttcggac agccggccga tcggctcggc cttcgcctcg
    28561 tgtacgacat cgcccacaac atggccaaac gcgagcagca cgacatcggc gggaagaagc
    28621 gctgggtgta tgtccaccgc aaaggggcca cccgggcctt cggcccgggg cacagggatg
    28681 tcccggagaa ataccgggcg gtcggccagc ccgtgatcat ccccggcgac atgggcaccg
    28741 ccagctacct cctggtcggc acggcccagg ccatgcggga gactttcggc tcctcctgcc
    28801 acggcgccgg gcggcggctg tcgcgcaccg cctcgatcaa ggagcaccga gccgaggacg
    28861 tgatccgcaa actccgcgag gggggaatct acctccgggc caagagcaag cgggtcgtcg
    28921 ccgaggaagc gcccggcgcc tacaagagcg tcgacgaggt cgtcgccatc tcccaccacg
    28981 ccggcatcac ccgcaaagtc gcccgcctga ctccggtcgg ggtcgtcaag ggatagaccc
    29041 ggcgcggacg cgctccgccc aatataaagc gggcgggtcg accgacccgc ccgcatggcg
    29101 tctgccgaat tcctgtccgg acgatgctac ttcaccagca gcatcttccg ggtttccgtg
    29161 aactcgccgg ccgagagccg atacagatac acgccgctgg acacgccgga ggcgttccac
    29221 tcgacgtggt gcgtgccggc cccgtacggc ccgctcgcca gcaccgccac ccgctgaccg
    29281 agcacgttga acacttcgag cgtcacgtcg cccggggtcg ggagcgtgaa gctgatcgtc
    29341 gtcgtcgggt tgaacgggtt cgggtagttc tgggcgagtt caaac
//
LOCUS       DAOPOE010000033        29057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2077_length_29057_cov_4.305565, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000033 DAOPOE010000000
VERSION     DAOPOE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29057
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_29057_cov_4.305565"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKM94_06160"
     CDS             complement(<1..231)
                     /locus_tag="PKM94_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOD66262.1"
                     /translation="MIFGVGLDIVDVERVAQQLSRHAGLREQVYTADEISYCSGRPHA
                     ARHFAARFAAKEAFLKALGLGLGAGIGLNEIEV"
     gene            complement(228..1205)
                     /locus_tag="PKM94_06165"
     CDS             complement(228..1205)
                     /locus_tag="PKM94_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOD66263.1"
                     /translation="MKRILITGAIGQIGTELTMALRQRYGADHVIATDIRMPADRSFL
                     ESGPFEFLDVLDPHHITRVMSIHQADTIYHLAAILSAVGENRPNQAWQVNVNGLYNVL
                     EAARQYHCAVFVPSSIGVFGNSTPLDRTPQVTIQRPETMYGVTKLAGELLCQYYFRRF
                     GVDTRGVRFPGIISYETEPGGGTTDYAVEIFIEAIKHKKYTCYLRPDTSLDMMYMPDA
                     IRAMVDLMEADPDRLEHRNAYNVTAMSFTPSELAREITQYVPGFAIEYQIDPVRQAIA
                     DSWPNSMDDSAARTEWGWRPEFDMRAMTRGMLEVLAKRHGHTDFRLVSR"
     gene            complement(1341..2183)
                     /locus_tag="PKM94_06170"
     CDS             complement(1341..2183)
                     /locus_tag="PKM94_06170"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOD66264.1"
                     /translation="MGRFLIRDIAVAGLVAAMYAVLSLAFAPISFGVYQVRVAEALSV
                     LPFLTGAAVPGLYAGCLLANIFGNMGWPDIVFGPFVTLAAALITYGLRRLGRRAALVL
                     AGLPVVLLWVGAVYLLSGFHLAVATVSGALLAAAAILPLVPRKGGRAGQQEYREWLGT
                     RLVIALIMALVAAIFLTATKDAYFIVAGGLLVIAAVAGAAVLAWSLVSDVGPAILLAP
                     LPPVVLNAFGVAAYLAPLLGVNYWFAAQMIGVGQLIACYVIGLPLLLLLRRRMVFAVP
                     PRET"
     gene            complement(2233..2871)
                     /locus_tag="PKM94_06175"
     CDS             complement(2233..2871)
                     /locus_tag="PKM94_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOD66265.1"
                     /translation="MYVLGLLKNQTAVHIADMARECGVSERTVYRDISSLIKLGFVVQ
                     YRDGYKLTPDADLPQTSLSGSEVALVNYCLRTNALAAHPYFRRRFAAIEQKLRGFAGG
                     ADRDDERAVEFESSESALNGATDPDLLAEFFRALQQRRKIRIKSKDAGANGGDLYTPI
                     SVGLRHQGQVLKVADDQGRVVEYVLDSLAWIGVTDVPYRRPAAADQASSSVV"
     gene            complement(3363..6170)
                     /locus_tag="PKM94_06180"
     CDS             complement(3363..6170)
                     /locus_tag="PKM94_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD66266.1"
                     /translation="MHNDEPQGGNSAAPQGVSPDLSARDAGLLLEQAGIGLMVMDPAG
                     TIRYANNAAPHLLGIEAGLVGRPMRDLLPLREHPRLAEILAAPGEDQPDAEWRILLSE
                     PGGRGPQREIALEYSWLRTGADRRLYATVRAIGGSAARDAGSSRVSGTPAPELDYRAI
                     ADCAYDWETWIGLDGRLVWVNPAVHRITGYTQEECLVMADYPDALLHPDDRARMVRSF
                     QGAMRGSTGNDIEFRVLRKDGRSVWVAVSWQPILSSAGVLLGHRSTIRDIGRRKAVEE
                     ELRESRRQLSTLLSNLPGMVYRSANEPEWPMDFVSDGCCALTGCEASEIMSGQVPYGN
                     LIHPDDKQMVWDAVQAGITIGRPFQIRYRIVRKDGAVRWVWEQGRMVEMEGGRSALEG
                     FIADITVPKLAEEELRRREQQYRSVVDSSPMGMHLYRLEPDGRLVFEGANPAADLILG
                     VCNEKFLGMTIDEAFPQLAQTEVPQRYRQTAAHGVTWQTEQLTYQDERITGAFHVVAF
                     QTAPGRMAAMFEDITERKRTQDALRESEHRFRLAFRTIPDAVAISRFDTGVFVEINEG
                     FTANTGYTRDDVVGVPADQVRVWVDPRVREHLVRMLFERGHMENLEAEFRRKDGTVLV
                     GLMSACLLTLNGRPHILSVTRNIQDLKDTQEALRREMERAQQYLDVAATLMMAVADDG
                     RVLMVNRKGADLLGYREEQLIGRSWIRDLLADEDQEQTEATFQKVVSGRMQGLDYYEC
                     RVVTASGEKRLIAWYLTDFRDRHGAIRGILTSGIDITERRRMEEEMQRIYEQLEHEHD
                     ALQKKNIALREVLGQIEGEKDAIKRLVVANIESDIVPTVQRLRGMASEPQGPLLDLLE
                     DSLAHIASEFAAGLKERFHGLTPRQVQICRLIKNGYSSKEIAESIGTSLLTVHKHREA
                     IRDKLGLKNRNISLNSYLQTL"
     gene            6576..8636
                     /locus_tag="PKM94_06185"
     CDS             6576..8636
                     /locus_tag="PKM94_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66267.1"
                     /translation="MGRLDQRRSREDSGSPIAMFFLKAGTKLLLGVIIALVFLTLAQC
                     TIKAPESPTWTTHAVVPVVNRTYPMGELLRKMDQEGLGIDDSGRVIFSLTEELDTVSL
                     DEDALATADLSFLAGERLGPVDLEIPALAPVTVRFAQLGGLGLPFIGDSVLVEDTTFT
                     IATALPPIASFRTATLARGRLDVTIDNELDLTLDNISLDLVETATGAIIGSGASPDPL
                     PSGAQAQVAIPLDGATIASDMTLVAGYHTLSDTVRAASGRYLRTGFAFGDTLQAVSAT
                     ARIPALARADSTEAPLAETDRLDSAYLASGVLNLTVHNATNLEATVTVSAPDFSYYGQ
                     PLAVVAPVAARSQAEVTVDLSGYALQPAVTATPQHIRVIAGAAMPGSGDALVAVDQTD
                     SFTVAARISDVTFHSVTGVFQSVRANFDGLSQDLDIPTGLEQVELTCAVLTLTIDNGI
                     DLPGAVNVSLTGDNGAQTTFVSDIAPRGLSTAATTTVVNDSAADFLYPLPTRLDVSGS
                     ISFGNGAYYGTITRSDFVVGRVSLYAPLEMVVHPTVVETDITGESIDQTDIDLITDHF
                     LSGRFVYRLTSRLPIGAHVNVFLGGDSASLFSAPQLRFDSLGLAAAPVGPGGIAIDTI
                     QSAEQEILLDSVDIRILKNPVLYAGHQIMLAGSDGQTVTFTADDYLSITGRLEIDYLF
                     DGDF"
     gene            8648..9904
                     /locus_tag="PKM94_06190"
     CDS             8648..9904
                     /locus_tag="PKM94_06190"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOD66268.1"
                     /translation="MNSHPRTAYLLIPAAAAALLLAPGTPAAAASARGMAMGDAYIGL
                     AAGVDAARYNPANLGLDGHRRSEVEIAGLGLNLTNNAFTLGDYNAYTGAFLTDDDKAD
                     LLSKVPPQGLALTADVQASAVSFAQGSFAFSTSGVGQAGVQLSRDLLELVLNGNTFGD
                     SVSITGSYSDAVAYAAAGISYGRALYTAGSRQFAVGGTLKYLRGLAVEQVVELEGLAA
                     AFATGLEGEGRLVARTASGGSGFALDLGAALKISDAYTAGLQVRNLVSRLSWSGTPEE
                     HRYIFSFDTVTVDNMDDDFVVAESGTRSLDPFTTTLPAVLTAGFARTSGKLLWAIDWQ
                     QGFRRAAGASTKPRLGLGLESRHLRFLPLRAGFAAGGEQPTAFSFGSGVAAGPLFFDA
                     AVMTGTDLSPYSAKGLNAALSIGLHL"
     gene            9923..11671
                     /locus_tag="PKM94_06195"
     CDS             9923..11671
                     /locus_tag="PKM94_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOD66269.1"
                     /translation="MSRTHDLLISLLGLGVAASLGLAVSACPPHPDLLAKIEAGAQPA
                     PYFLAHRPALRVQGVSTGMTMRRVPDGPPRLVPGQPPASGAYRALAVLVQFSDHPSAV
                     PAVYFDSLLFDSAGRSLRSYYRDVSYAQLDIVTLNLPSAHGWRTAPQTYAWYVNNQNA
                     LGDYPQNSQRLTEDIVDQIDPFVNFSQYDNNGDGYVDAVILIHSGSGAEKTGSNADIW
                     SHQWAITPRLKDGVYISDFTIQPELWVNPGDMTIGVYAHELGHAFGVPDLYDTDGTQS
                     RGLGVWCLMAYGSWLGPGGMGGSPSHPSAWSRIRMGFAAYTNVTANAVGQEIAAVESG
                     GPIYRLWSGGSPSPEYFLVENRQRTGYDSYLYSSGLFIWHIDETKATADNTDNTQEWW
                     PGAGMNPAEHYRVALEQADGLWELEKRLDYGDAGDPFPGALDRRVFDATTTPNSDSYL
                     AGTSFTKVDNISPSAAVMTADLIVGLAADVDSGDDNPTLPAAFELAQNYPNPFNPSTT
                     ISFELSGPMHVTLEIFNIVGQRVRTLMDGPAEAGRTERTWDGCDEAGRTAASGLYLYR
                     LTTGSGSRARKMLLLK"
     gene            complement(11765..11977)
                     /locus_tag="PKM94_06200"
     CDS             complement(11765..11977)
                     /locus_tag="PKM94_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOD66270.1"
                     /translation="MDRGFEPKILICSECGQEFVFTAAAQQYFAERGYMDDPKRCKTC
                     HTQYKKDQRSGSRTADYNHSEIHYPE"
     gene            complement(12120..14132)
                     /locus_tag="PKM94_06205"
     CDS             complement(12120..14132)
                     /locus_tag="PKM94_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOD66271.1"
                     /translation="MRKRTFLWFVILGLGLAAGSTASAVSDDPLALIAQDYRAGAITL
                     DQKVLLQIQAITSPAALPDRYVSAGLAASGGALTRCATPVILEIRREWELLQPETKAV
                     FSAAFQRAVTEAEYLSPSGWFKLHYDTTGADAVPPEDADMTGVPDLVEKCAAYLDSSR
                     TRHLDYGYRMPPPDSGLGGDDLFDVYFEETGAYGYAMPEGPGPEPWNDVYSYLVLNND
                     FLGFAPNYDPEGDQLGAAKATCAHEFHHCVQFAYDSNEDSWFMEMDATHIEDIVFDAT
                     DDNYNYLPTFFNAPERSLMEAGSHAYSTFLWGIYLAESFDTLLMPAIWEGARFQTAFA
                     AMTDTLFGQYGWTRDSAFADFTVWNYLTSGRDDGLHYGETYPQSVKIGRAHSMYPVLL
                     QTSPSNAAGYGACYIEFYPGSKIGRLEVTVNGDDARQWSAYLIKSTAPNVHTVEKIPL
                     ASGSYFGVGRVPEFESYYRVALVVANIMEYSSGAFFNYSAEVIPPYEVTCAAVTLDSS
                     VYSGGRRDFEFLITNVAPLSDIIDVTARDDSGWVVSGPAAYSLSPESDSVVRVQIAPP
                     EGTVLGSVSAVWCRAASRGNPAVVDSQLVFAQTVLQRGDVNFDGGIQVSDLTYFIAYL
                     FRGGAAPVPAVAAGDVSCDGGVDVRDLSALVAYLFRGESPAPCNPY"
     gene            complement(14377..16032)
                     /locus_tag="PKM94_06210"
     CDS             complement(14377..16032)
                     /locus_tag="PKM94_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66272.1"
                     /translation="MPSLLLVIAWSSLAALGLPAGAGALAPEADLDSLGPIVDSIVID
                     NRNVYDTDDPKYDNFLFRLANRIHIVTREHVIRRELLFRTGEPYPYEQAEESARNLRS
                     RLALYDAWIETETLPNGHLLVRVVTIDEWTLAFGPSVRTEGGDHEYEFLLTERNVLGR
                     NQEVRARYVFGNSEKDFVDLAYTNQRMFGRPVRAAGGYRTDPVNGVKWVELSHPYYNL
                     TQSFTYRAEVGASEVRRDVYSDDLLIGQSFREGDWCELGIGSRTGTYRRKLELSCRYY
                     YRFDRTVGTALIDTDPDLMEQALRSLPDDSAYHLVEVGTAFSNLDFTTLTRIDGFSYT
                     EDFELGYGLGISYGRAFLPDFDRHAYDRLSASVSHGRRFGSNLWYGSWRYTVWLRGER
                     DLRRLSDVYSRFYRRWFEFLTLAMGFSYRSDWRADGTEGLVMGGETGLRGYDQYFRTG
                     DRMAVLNLEARFFPDVRIHSAVFSPVLFTDFAQRWKRGEAFGTGDVYGAIGAGLRIAL
                     EHSARARLVRVDASYSEVNSWRFSISAEQYFIAPRSGFFLTSP"
     gene            16271..18004
                     /locus_tag="PKM94_06215"
     CDS             16271..18004
                     /locus_tag="PKM94_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD66273.1"
                     /translation="MEKKKIGDLLVEKGYVTAAQVEDALAEQTLTGRRLGEILVSRNA
                     LTEDQLVDVVSERLGIPKITLTAMVIDPEVIRRVPVGLARRYTLIPIFEIGATLTLAM
                     ADPLNIIAIDEIRYLTGKNIKRAIATPGEIKAAIDEFYSVADSLNQIIGAHAAPAGES
                     GAPRPGADVESESAVVRMVNLMIEQAAKDRASDIHIEPEENRLRIRYRINGAMREEAA
                     PPKAMQNELISRIKIAANLDVSEKRLPQDGRFMMTVDKAAIDLRVSTLPTIHGEKIVI
                     RLLDRRNLLLSFRQLGFGPTLETAWKTVIYRPEGLILISGPTSSGKTSTLYATLHEIN
                     SVEKNIITVEDPVEYSLPLIIQIQINEKAGLTFPTCLRAILRQNPDVIMIGEIRDPET
                     ARMAIRSALTGHTVFSTIHTNDAPSAIARLVDMDIERYLVASALKGVLAQRLVRVTCP
                     ECAAPYRPAESVLLQAGLRDRDSQFDFRKGVGCPKCKNTGFRGLTGIYEYVEVTPTIA
                     DMLMRNAPLNEIRESARRQGYVPLFEAGLEAVRAGRVCLEELLKETSNAEDYLRPGES
                     ASRRAVHAEPV"
     gene            17988..19202
                     /locus_tag="PKM94_06220"
     CDS             17988..19202
                     /locus_tag="PKM94_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD66274.1"
                     /translation="MQSRYKYRAADAAGRTREGVLLADSREQVLEHLAGQQLVPIRVA
                     AVAGRRGFSLLSFFSGPHYERLIAFTANLGTMYRAGVPLLRALALIRIGPAAGHFNRA
                     IDDIRLAVQAGRPLSQAMAAHDDLFARAYVAGVAAGEESGKLDEILAQLRTMLEQDLE
                     LSRAVKAGTRYPLMVVGALGAAALVLVLYVVPRFTAFYGQFNAQLPLPTRLLIGLQHT
                     VSAYWPALLMALVLAALAVRQAIGTERGKSWLDHRLLRLPIVGPLIIKSNVARFAAMF
                     HVLFQAGLPIIACLEILAETVKNSALAGEIRTMAGLFREGRDSRLAEHEFRYFPPLAL
                     QMMTIGLESGSLAEVLREVGEHYGKEVQYTSRQLTAVLEPILTFVMGIFVLILALAIF
                     LPMWNLIRVFNG"
     gene            19289..19714
                     /locus_tag="PKM94_06225"
     CDS             19289..19714
                     /locus_tag="PKM94_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD66275.1"
                     /translation="MNQLRNRRGFTLIELVIIIVILGILAAVAIPKYQDLSGEAREAS
                     ARSALGSMRSAVTIFYANQAVQTGTATWPSLADLQTRGTVMAQGLPPNPYQSNAPDSV
                     VDGTGQVRGTVIGNRGGWVYDPVSGELWANTDTHDENEW"
     gene            19704..20192
                     /locus_tag="PKM94_06230"
     CDS             19704..20192
                     /locus_tag="PKM94_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD66276.1"
                     /translation="MSGERIRPDPARSDRRLSVHPAGFTLVELVIIIVILGILAAVAI
                     PQLADMSGSAKTAATRKEMGELRRALVGSPEIVAGGRPVAAGFQGDVGRLPGRLSDLT
                     VRPDSVPPYDRLARYGWNGPYIDPSGGNYLSDAWGVPYVYDPDARILLSVGGGDTLRV
                     TL"
     gene            20200..21798
                     /locus_tag="PKM94_06235"
     CDS             20200..21798
                     /locus_tag="PKM94_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66277.1"
                     /translation="MRSLLKKFTAGAHAPSSPASTGPAERPLAPRGRFLIGRTLAGRV
                     SDDALSLAAVRHFGARRIVTDVLHADLPPGALPEAGSEFAISQVADFWSRHGGRGARF
                     AACVAGDGVTVRTFCLPRMSRADLASAVEIEASRQIPFPVGEFTCGFRPVARLEGEGG
                     GRCRVALLAATHRLLRQALVPLEQAAVPVQAVYAGAQAVGVLLTALPNFDPDRIYTLV
                     RLGLRESETAFYRAGNLEFSHHGGTGTAMLGTAGDEARREFFAETLASEIQTSLDYFA
                     GQHLRLHEPEVFVYGGAAGHTRLLRLLSERLGCPVEPFPLDRLAGIRLAGHIERDVAL
                     AALPAIAAALCRSDLVNLLPPDRRAEWRRRRVDALGRTAVAAVLLFLAAGGGFLYHDL
                     HVQQETDLALRQQLALYENSGAYRALSSLRTRLAAADSYLKASAKNPTSLALNLKELS
                     HLTPPEVRLTRYDFTPAAAGANLRLSGVVTSSDVPPEVVLAAFVDRLNAGRFFDSVTV
                     ERHVKRRQDETFILEFQLALRGRL"
     gene            21795..22355
                     /locus_tag="PKM94_06240"
     CDS             21795..22355
                     /locus_tag="PKM94_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66278.1"
                     /translation="MKPRVLIYGGIALALVVLWAFLVYTPYRHKAASARAAVRDTERR
                     IDEAHRLTLQLPAFLKAEQTAESLRLDLDARLYAHADVLALVDDLVARADRHRLRVAE
                     ITPPVEELLQLDRTAAAASPPFLNLEITVTGEFEQLGRFVADVEGSDYARGINRCIIA
                     GAPDGSAPLSLQLGLRALLGSRGGSA"
     gene            22352..22795
                     /locus_tag="PKM94_06245"
     CDS             22352..22795
                     /locus_tag="PKM94_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66279.1"
                     /translation="MSPARRRLFLYVLLGLAVVWGAYSFRRPRPIATPPPATGAAADT
                     AVVPRSDPPARQSDVSSLAARPWGEDPFRVRVRRTDREPAAPAPSWRLSGIVYSENHP
                     LAIINSRPVGVGDRVDGAVVIDINKRQVTLEADGRRFQINVAAKG"
     gene            22800..24080
                     /locus_tag="PKM94_06250"
     CDS             22800..24080
                     /locus_tag="PKM94_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66280.1"
                     /translation="MLTRTMFVCLCLGAAPAAGAEVTPDSAQLLTLSLENVAVADVLN
                     LIAVQNGLNLVVSGEVTGTVSLRLEEVSWTSALDAILLPMGYNYFVRGEVIIVKPFDQ
                     VSPGELDTRTVTLRFLDPVTAKKALEPLKSAQGQIIVLDKSPSAEAVALPAAETYRPN
                     RLLLADFPSVVDRMLAVLEEIDQPEPVVTIEARIIETNVDDDTRIGFTWPTVLTATLG
                     GDSASTGGSGLSGSSLAWDPNNGDLSWARLSAEQVSVMLHLLEQGGNSRLISDPHVTT
                     LANHEAEIKIQTVIPIATINRFTEGAVIQDIVTFQDEEVGISLRVTPRVNREGMITME
                     VIPVVEDIIDFTGPVDNQKPITTERSVRTTVAVAEGETLALGGLRKESELKQKQRVPL
                     LGRIPLLGNLLFTYTSNEKKTTDLIILITPRVLP"
     gene            complement(24049..24531)
                     /locus_tag="PKM94_06255"
     CDS             complement(24049..24531)
                     /locus_tag="PKM94_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOD66281.1"
                     /translation="MRRLIDNDYLTLLIRLFVGGVFIYASYYKIINPMDFAKSIWYYH
                     MVPGSLINLMAIVLPWWELVAGVCLVLGVWYRGTVWSMLLMTVMFGAALASAAIRGLD
                     IQCGCFKASVGAGDEAAATLVRDLGLFVLTLQLLFSRARRFFVGRARPLTEGRAALSR
                     "
     gene            complement(24519..25037)
                     /locus_tag="PKM94_06260"
     CDS             complement(24519..25037)
                     /locus_tag="PKM94_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD66282.1"
                     /translation="MALRQLIFIVTLSAVLGLAANLVSPHAIPYIGTWRELSSGDGPI
                     KPPEHAPGDPPYIGIDVAHVEYTGGGVIFIDARSPEEFACGTIPGAVNIPFDELPEGD
                     LTPYFDSALAGAPRDTRLITFCSGEECDLSLHLARNLQAVGYTDLLIFFGGSREWEKN
                     GLEMERRAKCDG"
     gene            25705..27192
                     /locus_tag="PKM94_06265"
     CDS             25705..27192
                     /locus_tag="PKM94_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66283.1"
                     /translation="MKATVLWIFIMLAAVLLSGTTGYTQVPGTINYQGRLTGTGGAPL
                     DTTVNMVFTIYTASSGGTALWTETRTGVVVADGLFSVALGSVNPINLSVFGGADRWLG
                     IKVGTDAEMTPRALLSAAPYAQVASWAKTSDTADYAMAGAASGWTDNGANVVLQSSSD
                     NVGIGTNAPQYKLHVAGTILMDNAVDQGVELRFAENGLRKWGIFYAPSDGRDLSIYDD
                     MGNRYLMTFDSGTGFVGIGTRTPTVPLDVAGKIKCSDDLSATERIFIDGVGGDNAEMR
                     LLEDGVGKWGMFFRPSDHRRLSIYDDMANRYVMTFDSGTGFVGIGTGTPVATLDVNGR
                     ARVASSLLVGDTMTCDIVKITGGADLAEPFDLHNAETIQPGMLLVIDPANPGKLTVAN
                     APYDHCVAGVVSGAGGIAPGLVMGQAETIASGKYPVAMTGRVYCLADADRAPIRPGDL
                     LTTSAVPGHAMRVTDFDRAQGAVIGKAMTALESGRGLVLVLVSLQ"
     gene            27209..28837
                     /locus_tag="PKM94_06270"
     CDS             27209..28837
                     /locus_tag="PKM94_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD66284.1"
                     /translation="MTITKQSICKAPPLPWLLIALALTVTAVTTSWSVPAEDVQRAAE
                     TWVRYVTAQPKPDAMVIRMEPYEAEGEVVAYIAHLAGGGYCLCSNDTLLLPIYLYNPS
                     GEYDLEVPAVQCILEGIAAKHRTIKTGVERDDPVVIERLPALVQRVSAWRSLIAGQVP
                     LGYGVYDDLRADPDSMRLPLTCKWDQPSPYNDFAPVLHPGTDSHCVAGCAPISAAQLL
                     YYWQWPTTGSGSKTKYYPRRYSPTWVERPYPNSITVPAGILHNRFEWSSAGGGKIRGW
                     GYWDFYVKDKASELSSDPAYQAALDAMFYAIPADSVPVSANFGGTTYDWALMEDVHTD
                     EVADPGDTAAAWLCRDVAIAISARYGYGGTSGAVSPDSLHKYFLYGTDGHHLPVNADT
                     AKAEIRWFRPVVAEGCSHSWLVYGYYNATGEFLHNLGWGPDNSAALGPIDSIVPCTTG
                     GDWMATMIAPEDYVRFVGNTIAGDGSPNSPHLNIDAALASAPDGATLVFKAGTTHISA
                     AADLIISRPLTLKGYNVIIKGAAKSPGLGKGEPE"
     gene            28834..>29057
                     /locus_tag="PKM94_06275"
     CDS             28834..>29057
                     /locus_tag="PKM94_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66285.1"
                     /translation="MKRPIGNWWWVLGAALLLMPLAGFMQPTATPPAPDASSAPLRDA
                     DAVAINWQVVASGGGPGVSANFLLNGTVGQT"
BASE COUNT         5046 a         9964 c         9016 g         5031 t
ORIGIN      
        1 cacctcgatc tcgttcaggc cgatgccggc gcccagcccg agaccaagcg ctttcaggaa
       61 ggcctccttg gcggcgaacc gggcggcgaa atgccgcgcg gcgtgcggcc gcccgctgca
      121 gtatgaaatc tcgtccgccg tatacacctg ttcccggagc cccgcgtgac gcgacagctg
      181 ttgcgcgacg cgctctacat cgacaatatc aaggccgact ccgaatatca ccgtgacacc
      241 aggcggaaat ccgtatggcc gtgccgcttg gccagaacct cgagcatccc gcgggtcatc
      301 gcccgcatgt caaattccgg ccgccacccc cactcggtgc gggcggccga gtcatccatc
      361 gagttcggcc aggagtcggc aatcgcctgc cgcaccgggt cgatctggta ctcgatcgca
      421 aaccccggca catactgagt gatctcgcgg gccagctccg agggggtgaa actcatggcc
      481 gtgacgttgt aggcattccg gtgctccagg cggtccgggt cggcctccat caggtcaacc
      541 atcgcccgga tcgcatccgg catgtacatc atgtccagac tcgtgtccgg cctcaggtaa
      601 cacgtgtact tcttgtgctt gatcgcttcg atgaaaatct cgaccgcgta atcggtggtg
      661 ccgccgcccg gctcggtctc gtaggagatg atgccgggga aacggacgcc gcgggtgtcc
      721 acgccgaacc gccggaaata gtactggcag agcaactccc cggccagctt ggtcacgccg
      781 tacatcgtct ccggccgctg aatcgtcacc tgcggcgtgc gatccagagg ggtcgagttg
      841 ccgaacaccc cgatcgaact ggggacaaac acggcgcagt ggtactggcg ggccgcctcg
      901 aggacgttgt acaggccgtt gacattgacc tgccaggcct gattggggcg gttctcgccg
      961 accgccgaga ggatagccgc aagatggtag atggtgtcgg cctggtggat cgacatgacg
     1021 cgggtaatgt ggtggggatc aagaacatcg aggaactcaa acggtcccga ttcgaggaac
     1081 gaccggtcgg cgggcatccg gatgtcggtt gcgatgacgt ggtcggcccc gtagcgttgc
     1141 cgcagggcca tcgtgagctc cgtaccgatc tgaccgatcg cgccggtgat gagaatccgt
     1201 ttcatgctgc tccaccagtt gcagttggcg tccggcgcat acaaccagcc gccggcgcgc
     1261 aggttatctc aattttcggc gtacataata accgccggag gtcgccctgg caaggggaaa
     1321 aagtccttcc ctgtccgggt ttacgtctcg cgcggcggaa cggcgaagac catgcggcgg
     1381 cgcagcagca gcaacagcgg cagaccgatg acgtagcagg cgatcagttg ccccaccccg
     1441 atcatctggg ccgcaaacca gtagttcacg ccaagcagcg gcgccaggta ggccgccacc
     1501 ccgaacgcat tgagcaccac tggcggcagg ggagccagca ggatcgccgg accgacatcc
     1561 gaaaccaggc tccaggcgag aacagcggcg ccggccacag cggcgatcac cagcagtccg
     1621 ccggcgacga tgaaataggc gtccttcgta gcagtcagaa aaatcgccgc caccagcgcc
     1681 atgatgagcg cgatcaccaa ccgggtcccg agccactcgc ggtactcctg ctgcccggcc
     1741 cgtcccccct tgcggggaac cagcggcagg atcgccgccg cggccagcag cgcgcccgac
     1801 acggtcgcca ccgccaggtg gaaaccggaa agcaggtaga cggccccgac ccagagcagc
     1861 acgaccggca gcccagccag gaccagcgcg gcccggcggc cgagacgccg cagaccgtag
     1921 gtgatgagcg ccgccgccag cgtcacgaac ggcccaaaca cgatatcggg ccagcccatg
     1981 ttgccgaaga tgttggccag gaggcacccc gcatacagcc ccggcaccgc cgcccccgtc
     2041 agaaacggca ggacactcag ggcctcggcg acgcgcacct ggtagacgcc gaatgatata
     2101 ggcgcgaaag ccagactgag caccgcgtac atggcggcca ccagcccggc gacagcgatg
     2161 tcccgaataa gaaaccggcc cattcagtga agaatgaacc ggttttgggg aggtgacgtc
     2221 aactctgaac tgtcagacca ccgaagagga tgcctgatcc gcggccgccg gccggcgata
     2281 cggcacatcg gtcacgccga tccaggccag cgaatcgagc acgtactcga cgacacgtcc
     2341 ctggtcgtcg gccaccttca gcacctgccc ctgatggcgc agcccaaccg agatcggggt
     2401 atacaggtca ccgccgttgg cgccggcgtc cttggatttg atgcgaattt tacgccgctg
     2461 ctggagagcg cggaagaact cggcgagcag gtcggggtcg gtggccccat tgagcgccga
     2521 ttcgctcgac tcgaactcca ccgcccgctc gtcgtcgcgg tccgcgccgc ccgcgaaccc
     2581 gcgcagtttc tgctcgatcg cggcaaagcg ccggcggaaa tacggatggg ccgccagcgc
     2641 gttcgtccgc aggcagtagt tgaccagcgc gacttccgac ccgctcagcg acgtctgcgg
     2701 cagatcggca tccggagtca gcttgtagcc gtcccggtac tggaccacaa agcccagctt
     2761 gatgagggag ctgatatcgc gatacacagt ccgctccgat acgccgcact cgcgcgccat
     2821 atcagcgata tgcacggccg tctgattctt taacaggccg agtacgtaca tcagtcgttc
     2881 cgacttagtc aagataagtc ccctttgtgc ctgttgacgc ggcggtccgg tccccggcca
     2941 atgccttgct ccgcccgccc gcggcagtgc gggccccggg ccggagtcga ccggaggcag
     3001 acggtcccgt caatgtgtga gttctgcgtt ctcaaaagct gattcctctt taccacctgc
     3061 gccttaggag caaattgtgg aggcgtcggt ctcgttctct cattttcccg gcaaccgggt
     3121 caggcagctt ttccggtgca catccctggc caagccgtct gtccacgttg tcatcgttgt
     3181 cgtacccgct gtcagttctg tcggctgcac gctctcgaca gtggtctcgc gcacgctgcc
     3241 gtcgccagct gcctgacccg gtgtccggga gtttcggcgg tttctgacag gaaaatcggt
     3301 gaattgacgg aaaaggtcaa taagagtaga ttaccagagt attgcgtatt cataatacgc
     3361 aatcatagtg tctgcagata agaattgaga cttatgttgc ggttctttag cccaagtttg
     3421 tcacgaattg cctcgcggtg cttgtgcact gtaagcagcg atgtcccaat tgattccgca
     3481 atctccttgg agctgtaccc gttcttgatc aggcggcaga tctgtacctg ccgcggcgtc
     3541 agaccgtgaa accgctcctt tagaccggcg gcaaattctg acgcaatatg cgcgaggctg
     3601 tcctccagca ggtcgagcag gggcccctgg ggctcgctcg ccatgcctcg gaggcgctgc
     3661 acggtaggga caatatccga ctcgatgttg gcgaccacca gacgcttgat ggcgtccttt
     3721 tccccctcga tctgtccgag gacctcccgc agcgcaatgt tcttcttctg cagggcatcg
     3781 tgctcatgct cgagctgctc gtatatgcgc tgcatctcct cttccatccg gcgccgctcg
     3841 gtgatgtcga tcccggaagt caaaatgccc cggatagctc cgtgtctgtc ccggaagtcg
     3901 gtcaggtacc aggcaatgag ccgcttttcg cccgaggcgg tcactacgcg gcactcgtag
     3961 taatccagcc cctgcatccg ccccgacacc actttctgga aggtcgcttc cgtctgctcc
     4021 tggtcctcgt cggccaggag atcccgaatc cacgaccgcc cgatgagctg ttcttcccgg
     4081 taccccagca ggtccgctcc cttgcggttg accatgagca ctcggccgtc gtccgcgacc
     4141 gccatcatca gcgtggccgc cacatccagg tactgctgcg cccgttccat ttcgcggcgg
     4201 agggcctcct gagtgtcttt gagatcctgg atgttgcggg tgaccgagag aatgtgcggc
     4261 cggccgttga gagtcagcag gcaggccgac atgagcccga ccagcaccgt gccgtctttg
     4321 cgccgaaact ccgcttccag gttctccatg tggccgcgtt cgaagagcat gcgcaccaga
     4381 tgttcgcgga cccgcgggtc gacccagacg cggacctggt cggccggcac gcccacgacg
     4441 tcgtcgcgcg tatacccggt gttggccgta aacccctcgt tgatctcgac aaagacgccg
     4501 gtgtcgaagc gggagatcgc cacggcgtcg gggatcgtgc ggaaagccag tcggaaacgg
     4561 tgttcgctct cgcgcaaagc gtcctgcgtc cgcttccgct ccgtgatatc ctcaaacatg
     4621 gccgccatgc gccccggcgc agtctggaac gcgaccacgt ggaaagcgcc cgtgatgcgc
     4681 tcgtcctggt aggtcaactg ctcggtctgc cacgttacgc cgtgggccgc cgtctgcctg
     4741 taacgctgcg ggacttcggt ctgcgccagt tgtggaaacg cctcatcgat cgtcattccg
     4801 aggaatttct cgttgcagac gccgaggatc aggtccgcgg ccggattggc cccctcgaac
     4861 accagccgcc cgtccggctc cagccggtac aggtgcattc ccatcggcga cgaatccacc
     4921 accgaacggt actgctgctc gcggcggcgc agctcctcct cggccagttt tgggaccgtg
     4981 atgtcggcaa taaacccctc cagcgctgac cgtcccccct ccatttcaac catccgcccc
     5041 tgctcccaga cccaccggac cgcgccgtct ttgcgcacaa tccggtacct gatctggaac
     5101 ggccggccga tcgtgatccc ggcctgcaca gcgtcccaca ccatctgctt gtcgtcgggg
     5161 tggatgaggt tgccgtaggg aacctggccc gacatgattt cgctggcctc gcagccggtc
     5221 agggcacagc acccgtcgct gacgaaatcc atcggccact ccggctcgtt ggcgctgcgg
     5281 tacaccatcc cgggcagatt gctcagaagc gtgctcagtt ggcggcggct ttcgcgaagt
     5341 tcctcctcga ccgccttgcg ccgcccgata tcccggatcg ttgaccggtg gccgagcagc
     5401 accccggccg aactgagaat cggctgccac gacaccgcca cccagacgga ccgcccatcc
     5461 ttgcgcaata cccggaactc gatgtcgttg cccgtgctcc cgcgcatcgc cccctggaaa
     5521 ctgcggacca tgcgggcgcg gtcatccgga tgcagcagcg cgtcgggata gtcggccatg
     5581 accaaacact cttcctgcgt gtacccggtg atgcggtgga cggccgggtt cacccacacc
     5641 aagcggccgt cgagccctat ccatgtctcc cagtcgtagg cgcagtcggc gatcgcccgg
     5701 taatcgagct ccggcgccgg agttccgcta actcgcgacg accccgcgtc tcgcgccgcc
     5761 gaaccgccga tcgcgcgcac ggtcgcatac aggcgccgat ccgcgccggt ccgcagccaa
     5821 ctgtattcga gggcaatctc gcgctggggg ccacggccgc ccggttcgct gaggagaatc
     5881 cgccattcgg catcgggctg gtcctctccc ggcgccgcca ggatttctgc cagccgcgga
     5941 tgctcccgca ggggcaggag atccctcatc gggcggccga ccagccccgc ttcgatccca
     6001 agcagatgcg gcgccgcgtt gttggcgtag cggattgtgc cggcggggtc catgaccatc
     6061 aggccgatac cggcctgctc cagcagcaat cccgcatcgc gtgccgacag atccggggaa
     6121 actccctgcg gagcggcact attccccccc tgcggttcgt cgttgtgcac tcagcctttc
     6181 ctgttatggt ctttgttcgg gggaatctgg gaccggacct gtcccggtac gtatcaggat
     6241 ccccccggaa cgaaccctga agcgttggag gccgggacag aatggcagat tgagcctcgc
     6301 gagtcaatcg aaatcatcaa cctgtcagct tctgtcatcc gtaacgttgc cccggcaggc
     6361 gacgttcccg caatccggcc gcgcagtggg aattggggga tcgcgcccac agtttccgct
     6421 gtcttctcac cagcaccggt cccgcgccga agccgcccgg ctgacccaag tcgctgccgt
     6481 cgtattgctt acgcgagcgt cggcacccgg catccggttt gcacccccct gttgtggaaa
     6541 cagtcctgaa actgctgtgc cgtaaggggg accctatggg gcgtttagac caacgacggt
     6601 cgcgcgagga ctccggttcg ccgatcgcca tgttcttcct caaagccggc accaagctcc
     6661 tgcttggcgt catcatcgca ctggtcttcc tcacgctggc ccagtgcacg atcaaggctc
     6721 cggaatcacc cacctggacc acccacgcgg tggtcccggt ggtgaaccgg acctacccca
     6781 tgggagaact gctccggaag atggatcagg aggggctcgg aatcgatgat tccggccggg
     6841 tcattttcag cctcacggaa gaactggata cagtgagcct cgacgaggac gcgctggcca
     6901 cggccgattt gtccttcctg gccggcgagc gtctcggccc cgtagacctc gaaattcccg
     6961 ccctggcccc ggtgacggtc cgcttcgccc aactgggcgg cctcggtctc cccttcatcg
     7021 gcgactcggt tctggtggaa gacaccactt tcaccattgc gacggccctg ccgccgatcg
     7081 cgagtttccg caccgcgacg ctggcgcgcg gacggctcga cgtcacgatc gacaatgaac
     7141 ttgacctgac cctggacaac atcagcctcg atctggtcga gacggcaacc ggcgcgatca
     7201 tcggctccgg cgcctccccc gatcccctgc cgagcggtgc gcaggcgcag gtggcgattc
     7261 ccctcgacgg ggcgaccatt gcgagcgaca tgacgctggt ggcggggtac cacacgctga
     7321 gcgacacggt ccgggccgcc tccgggcgct acctccgcac cggcttcgct ttcggcgaca
     7381 cgctccaggc ggtgtcggcc acggcgcgca ttccggctct cgcccgcgcc gactcgaccg
     7441 aggcgccgct ggcggaaacg gaccgtctcg actcggccta tctggcgagc ggggtgctga
     7501 acctgacggt gcacaacgcc accaatctcg aggcaaccgt caccgtctcg gcgccggatt
     7561 tttcctacta cggtcagccg ctggcggtcg tcgcaccggt tgccgcgcgc tcgcaggctg
     7621 aggtgacagt cgacctgtcc ggctacgcgc tgcagcccgc cgtgaccgcg accccgcagc
     7681 acatccgggt gatcgccggc gcggccatgc ccggctcagg cgatgcgctg gtggcggtcg
     7741 accagaccga cagcttcacc gtcgcggccc gcatttccga cgtcactttc cactcggtca
     7801 ccggcgtctt tcagtcggtg cgggcgaatt tcgacggcct ctcgcaggat ctcgatatcc
     7861 cgaccggtct ggagcaggtc gaactcacct gcgccgttct cacgctgaca atcgacaacg
     7921 gcatcgacct gccgggggca gtcaacgtga gtctcaccgg cgacaacggg gcgcagacaa
     7981 ccttcgtgag cgacatcgcc ccccgcggcc tgagcaccgc ggccaccacg acagtcgtca
     8041 acgactccgc ggccgatttc ctctacccgc tgccgacgcg cctggacgtc tccgggtcga
     8101 tcagtttcgg caacggcgcg tactacggca cgatcacgcg gagtgatttc gtcgtcgggc
     8161 gggtcagcct ctacgctccg ctggagatgg tggttcaccc gaccgtcgtc gaaaccgaca
     8221 tcaccggcga gtccatcgac cagaccgata tcgacctcat caccgaccac ttcctctccg
     8281 ggcgctttgt ctaccgattg accagccggc tgccgatcgg cgcgcatgtc aacgtgtttc
     8341 tcggcggcga ctccgcctcc ctgttcagcg cgccgcaact gcggtttgat tccctgggtc
     8401 tggcggccgc tccggtcggc cccggcggca tcgccatcga caccatccag tccgccgagc
     8461 aggaaatcct cctcgactcg gtcgacatca ggattctgaa aaaccccgtc ctctacgcgg
     8521 ggcatcagat catgctggcg ggctccgacg ggcagaccgt gacgttcacg gccgacgatt
     8581 acctctcgat caccgggcga ctcgagattg actacctctt tgacggcgat ttttagggag
     8641 cgcacgcatg aattcacacc cgcgcacagc gtatctgctg atcccggccg cagccgccgc
     8701 cctgctcctt gcgccgggaa cgcctgcggc ggcagcctcg gcccgcggga tggccatggg
     8761 ggacgcctac atcggcctgg ccgccggcgt cgacgcagcc cgctacaatc cggccaatct
     8821 cggtctggac ggccaccgcc ggagcgaggt ggagatcgcc ggccttgggc tgaacctgac
     8881 caacaacgcc ttcaccctcg gcgattacaa cgcctacacc ggcgccttcc tgaccgacga
     8941 cgacaaggcc gacctcctga gcaaagtgcc gccgcaggga cttgcgctca cagcggacgt
     9001 ccaggcctcg gccgtctcgt tcgcccaggg ctcattcgcc tttagcactt cgggcgtggg
     9061 gcaggccggc gtccagctct cccgcgacct gctcgaactc gtgctcaacg ggaacacttt
     9121 cggcgattcg gtgagcatta ccggttccta ttcggacgcc gtcgcctacg ccgcggcggg
     9181 gatctcgtac gggcgcgcgc tctacacggc aggctcccgc cagttcgcgg tcggggggac
     9241 gctgaaatat ctccgcgggc tggccgtgga gcaggtggtc gaactcgagg ggctggccgc
     9301 cgctttcgcc accggacttg aaggcgaggg gcgcctcgtc gcccgcaccg cgagcggcgg
     9361 ttccggcttc gccctcgacc tcggcgccgc cctgaaaata tcggacgcct ataccgccgg
     9421 cctccaggtg cgcaacctcg tcagccgcct gtcgtggagc ggcacgcccg aagagcaccg
     9481 ctacattttc agcttcgaca ccgtcaccgt ggacaacatg gacgacgatt tcgtggtcgc
     9541 ggagagcggg acgcggtccc tcgatccgtt caccacgact ctcccggcgg tcctcaccgc
     9601 cggattcgcc cgcacctccg gaaagctcct ctgggcgatc gactggcagc aggggttccg
     9661 ccgggcggcg ggggcctcca ccaaaccgcg gctgggtctc ggcctggagt cgcggcacct
     9721 tcggttcctc ccgctgcgcg ccgggtttgc cgccggcggc gagcagccga ccgccttttc
     9781 gttcggctcg ggcgtggccg ccggtccgct tttcttcgac gcagcggtga tgaccgggac
     9841 cgacctttcg ccctattcgg ccaagggtct gaacgccgcc ctctctatcg ggctgcacct
     9901 ctaaagaagg agattgggga acatgtcgcg cacacacgac ctgctcatat ccctgctggg
     9961 cctgggagtc gccgcctccc tcgggctggc ggtctcggcc tgtccgccgc atcccgacct
    10021 gctggcgaag atagaagcgg gcgcacagcc ggctccgtat ttcctggccc accggccggc
    10081 tctccgcgtc cagggcgtgt cgaccggcat gaccatgcgc cgcgtccccg acggtccgcc
    10141 gcggcttgtc ccgggccagc cgcccgcgtc cggcgcctat cgggcgctgg ccgtcctggt
    10201 ccagttctcc gaccatccct cggccgtccc ggcggtctat ttcgactcgc tcctgttcga
    10261 ctcggcgggc cggtcgctgc gcagttacta ccgcgatgtg tcctacgcgc agctcgacat
    10321 cgtgaccctc aacctcccct ccgcccacgg gtggcggacg gccccgcaga catacgcgtg
    10381 gtacgtcaac aaccagaacg ccctcggcga ctacccgcag aacagccagc ggctgacgga
    10441 ggatatcgtc gaccagattg acccctttgt gaatttctcg caatatgaca acaacggcga
    10501 cggctatgtt gacgccgtga ttctgatcca ctccgggtcg ggggccgaga agaccggcag
    10561 caacgccgac atctggtccc accagtgggc catcacgcca cgtctcaaag acggcgtgta
    10621 tatttccgac ttcaccatcc agccggagct gtgggtgaat ccgggcgaca tgacgatcgg
    10681 cgtctacgcc cacgagctgg gccacgcctt cggcgtgccc gacctgtacg acaccgacgg
    10741 cacgcagtcg cgcggcttgg gcgtgtggtg cctcatggcg tacggatcct ggctcggtcc
    10801 cggcggcatg ggcggctctc cctcccaccc ctcggcctgg agccgcatcc ggatgggatt
    10861 cgccgcctac acgaatgtga cggcgaacgc ggtcgggcag gagatcgccg cagtcgaaag
    10921 cggcggaccc atctaccgcc tgtggtcggg aggctcgccg agccccgagt acttcctcgt
    10981 cgagaaccgc cagcggaccg gctatgactc gtacctgtac tcctcgggcc tgttcatctg
    11041 gcatatcgat gagaccaagg cgaccgccga caacaccgac aacacccagg agtggtggcc
    11101 gggcgcgggg atgaatccgg ccgaacacta ccgggtggcg ctggaacagg cggacggcct
    11161 ctgggagctg gagaagaggc tcgattacgg cgacgccggc gatcctttcc ccggcgctct
    11221 cgaccgccgg gtcttcgacg cgacgacgac cccgaattcc gactcctacc tcgccggcac
    11281 ctcgtttacg aaagtggaca acatttcgcc ctcggccgcc gtgatgaccg ccgatctcat
    11341 tgtcgggctg gcggccgatg ttgattcggg cgacgacaat cccacgctgc cggccgcgtt
    11401 cgagctcgcg cagaactacc ccaacccgtt caacccgagc accaccatca gtttcgaatt
    11461 gagcggcccg atgcacgtga cgctggagat cttcaacatt gtcgggcagc gcgtgcggac
    11521 cctgatggac gggccggcgg aggcggggcg gacggagcgg acgtgggacg ggtgcgatga
    11581 ggccgggcgg acggccgcct cgggtctcta cctgtaccgc ctcaccaccg gctcgggcag
    11641 ccgggcgcga aaaatgctgt tgctgaaata gctctccccc ctcagttcgg ctcaccggcg
    11701 gcggcaatgc cgccgccctg cggaccccgg cgaaaaaccg gggttctctt ttggccgccg
    11761 cccgctactc gggatagtgg atctcggagt ggttgtagtc ggccgtccgg ctgccgctcc
    11821 gctgatcctt cttgtactgc gtgtggcagg tcttgcagcg cttcgggtcg tccatgtacc
    11881 cgcgctcggc aaagtactgc tgcgcagcgg cggtgaacac gaactcctgt ccgcactcac
    11941 tgcaaatgag gattttaggc tcgaatccgc gatccatgtt tccccctctt tcgcgttccg
    12001 cacatcttcc ttgacgagca aaacgacgga aacaatggcg aaagaagcaa agtctatcta
    12061 cttttgcctg aattgatacc ggggcggtgc acccgtgtca acaaaaaacc ggcaattgat
    12121 cagtacggat tgcagggagc cgggctctcg ccgcggaaca ggtaggcgac gagggcggac
    12181 aggtcgcgca catccacccc gccatcgcag gaaacgtccc cggccgcgac cgcggggacc
    12241 ggcgcggcgc cgccgcggaa gagataggcg atgaaatagg tcaggtcgct cacctggatg
    12301 ccgccgtcga aattcacgtc cccccgctga agaaccgtct gcgcgaacac caactgggaa
    12361 tcaaccaccg cggggttgcc ccgcgaggcg gcccggcacc acaccgccga cacgctcccg
    12421 agcactgtcc cctcgggcgg cgcaatttgc acccgcacca ccgaatccga ctccggactc
    12481 aggctgtagg ccgccggacc ggacaccacc catcccgagt cgtcccgggc cgtcacgtca
    12541 atgatgtcgg agagcggggc gacgttggtg atgaggaact cgaaatcgcg gcgcccgccg
    12601 gagtagaccg acgaatccag ggtcaccgcc gcacatgtca cctcgtacgg ggggataacc
    12661 tcggccgagt agttgaagaa cgcgccggag gagtactcca tgatgttggc caccacgagc
    12721 gcgacccggt agtaggactc aaactccggc acccggccga ccccgaagta ggaaccggac
    12781 gcgagcggga tcttctcgac cgtgtgcacg ttcggcgccg tcgacttaat caggtacgcc
    12841 gaccactgcc gggcatcgtc gccgttcacc gtgacctcca ggcggccgat cttggagccg
    12901 gggtagaatt cgatataaca ggcgccgtag cccgccgcgt tggagggcga cgtctgcagc
    12961 aacaccgggt acatactgtg cgcgcgcccg atcttcaccg actgcgggta ggtctcgccg
    13021 tagtgcagac cgtcgtcgcg ccccgaggtc aggtagttcc agaccgtgaa gtcggcaaag
    13081 gcgctgtcgc gggtccagcc gtactgcccg aagagcgtgt ccgtcatggc cgcgaaggcc
    13141 gtctggaagc gcgccccttc ccagatcgcc ggcatgagca aggtgtcgaa actctcggcc
    13201 aggtaaatgc cccagaggaa ggtcgagtag gcatgggacc cggcctccat cagcgaccgt
    13261 tccggggcgt tgaaaaacgt cgggaggtag ttgtagttgt cgtcggtggc gtcaaagacg
    13321 atgtcctcga tgtgcgtggc gtccatttcc atgaaccagg agtcctcgtt gctgtcatac
    13381 gcaaactgca cgcagtggtg gaattcgtgc gcgcaggtcg ctttggccgc ccccagttga
    13441 tccccctccg gatcatagtt gggagcgaaa ccgagaaagt cgttattgag cacgaggtag
    13501 ctgtagacgt cgttccaggg ctccggaccg ggaccctccg gcatcgcgta gccatacgcc
    13561 cccgtctcct cgaagtagac atcgaacagg tcatcgccgc cgagcccgct gtccggcggg
    13621 ggcatccggt agccgtagtc caggtgccgg gtccgcgagc tgtcgagata agctgcgcac
    13681 ttctcaacga gatccggaac tccggtcatg tccgcatcct cgggcgggac cgcgtccgct
    13741 cccgtcgtgt cgtagtggag cttgaaccag ccgctcgggg acaggtactc cgcctccgtg
    13801 accgcccgct ggaaggccgc gctgaagacc gctttcgtct ccggctgcag caattcccac
    13861 tcgcgccgga tctccagaat taccggggtc gcgcaccggg tgagcgcgcc gccgctcgcg
    13921 gccagcccgg cggaaacgta acggtcgggc agagccgcag ggctcgtgat ggcctggatc
    13981 tgcagcagca ctttctggtc gagcgtgatt gcgccggccc ggtagtcctg cgcgatgagc
    14041 gcgagaggat catcggaaac agcgctcgcc gtcgacccgg cggcgagccc gaggccaagg
    14101 atgacgaacc agaggaacgt gcgcttgcgc atcaattctc cacccggagc gcccggggcg
    14161 cttccttacc atcagagtat cggtccgacc gccgacatat ccagtgcaac gtgcgtaaca
    14221 gggctttttc ttgcttccgc agaccttggg cggaccacga agtattctac caacagcggc
    14281 cgatcctgcg ccgttcccgc cacgagctcg cgggcaaacg ggagcgcggc gcgccggtgg
    14341 ggtagatttc cacggtccac cataatttat tgtatttcag gggcttgtca agaagaatcc
    14401 ggagcggggg gcgatgaaat actgctcggc cgagatcgaa aaccgccagg aattcacctc
    14461 cgaataggag gcatccaccc gcaccagccg ggcccgggcc gagtgctcca acgcaatccg
    14521 caggccggcc ccgattgccc cgtagacgtc ccccgtcccg aacgcctccc cccgcttcca
    14581 ccgctgcgca aagtccgtga acagtaccgg tgagaacacg gccgagtgta tccgcacgtc
    14641 ggggaaaaag cgcgcctcca ggttcaaaac cgccatccgg tcgccggtgc ggaagtactg
    14701 gtcataaccg cgcagcccgg tctcccctcc catgaccaga ccctcggtgc cgtcggcccg
    14761 ccaatcggac cggtagctga accccatggc cagcgtgagg aactcgaacc accggcgata
    14821 gaagcggctg tagacgtcgc tcagccgccg gagatcgcgt tcccccctga gccacaccgt
    14881 gtaccgccag gaaccgtacc acagattgga cccaaaccgg cggccatggg aaacgctcgc
    14941 cgacagccgg tcgtaggcat gccggtcaaa atcgggcagg aaggcgcggc cgtaggagat
    15001 gcccaggcca taacccagtt caaaatcctc ggtatacgag aacccgtcaa tccgcgtcag
    15061 cgtcgtgaag tcaaggttgg agaaggcggt ccccacctcg acgaggtggt aggcggagtc
    15121 gtccggcagc gatcgcagcg cctgctccat cagatcggga tcggtgtcga tcagcgcggt
    15181 ccccaccgtc cggtcaaacc ggtagtagta gcggcaggaa agctccagct tgcgccggta
    15241 ggtgccggtc cgggatccga tgcccagttc gcaccagtcc ccctcccgaa acgactgccc
    15301 gatgaggagg tcgtcgctgt atacgtcccg gcggacttcc gacgcgccca cctcggcccg
    15361 gtaggtgaag ctctgggtga ggttgtagta ggggtggctc agctcgaccc atttcacgcc
    15421 gttcaccggg tcagtgcggt acccgccggc cgcccggacc gggcggccga acatgcgctg
    15481 gttcgtgtag gcgaggtcga cgaagtcttt ctcgctgttg ccgaagacgt agcgcgccct
    15541 gacctcctgg ttgcgcccga gcacgttgcg ctcggtcagc agaaactcgt attcatgatc
    15601 cccgccctcg gtgcgcacgc tcggcccgaa cgccaacgtc cattcgtcga tcgtcaccac
    15661 ccgcacgagc aggtgtccgt tgggcagggt ttccgtctcg atccaggcgt cgtacagagc
    15721 caggcgcgac cggagattgc gggccgactc ctccgcctgt tcataggggt agggctcgcc
    15781 ggtgcggaac agaagctccc gccggatcac gtgctcgcgg gtgacgatgt gaattcggtt
    15841 ggcgaggcgg aagaggaagt tgtcgtactt ggggtcgtcg gtgtcgtaga cgttgcggtt
    15901 gtcgatgacg atggagtcga cgatcgggcc gaggctgtcg aggtcggcct ccggcgccag
    15961 cgcgcccgcc ccggcgggca gccccagagc cgcgagactc gaccaggcga tgacaagcag
    16021 aagtgacggc aaatgctgca atccgttctc cagactcctc tccccaataa acggcccctt
    16081 gctgcctttg taaacaacta cgcgcctccg ggatccatcg gctgccgccg ccgccggccg
    16141 ctgcggaccg gccaaaagcc ggcggcccgg tcccgcctcc ggccccgcgc ggttcagcca
    16201 tcatcggtcc ccgccgatat aacgagcggc ggcccgttgg cgccgaaacc cgtatcggaa
    16261 ccggaacgct atggaaaaaa agaaaatcgg cgacctcctg gtcgaaaagg gctacgtcac
    16321 cgccgcccag gtcgaggacg cgctggccga gcagacgctc accggccggc gcctgggcga
    16381 aattctcgtg tccagaaatg ccctcaccga ggaccagctc gtcgacgtcg tctccgaacg
    16441 gctgggcata ccgaagatca cgctcaccgc catggtcatc gaccccgagg tgatccgccg
    16501 tgttccggtc ggcctcgccc gccgctacac cctcattccg atcttcgaaa tcggggccac
    16561 cctgacgctg gcgatggccg atccgctgaa tatcatcgcc atcgacgaaa tccgctacct
    16621 taccggcaag aacatcaagc gggccatcgc caccccgggg gagatcaagg ccgcgatcga
    16681 cgaattctac tcggtggccg actcgctcaa ccagatcatc ggcgcccacg ccgccccggc
    16741 cggcgagtcg ggcgcgcccc gtccgggcgc cgacgtcgaa tcggagagcg ccgtcgtgcg
    16801 gatggtcaac ctcatgatcg agcaggcggc caaggaccgc gcctcggaca tccacattga
    16861 gcccgaggaa aaccgcctgc gcatccgcta ccgcatcaac ggcgccatgc gcgaggaggc
    16921 ggccccgccc aaagccatgc agaacgaact gatctcgcgc atcaagatcg ccgccaacct
    16981 cgacgtctcc gagaagcgcc tcccgcagga cggccgcttc atgatgaccg tcgacaaggc
    17041 cgcgatcgac ctccgcgtct cgaccctgcc gacgatccac ggcgagaaga tcgtcatccg
    17101 cctgctcgac cggcgcaacc tgctgctctc gttccggcag ttggggttcg gcccgaccct
    17161 cgagaccgcc tggaaaacgg tcatttacag gccggaaggc ctcatcctga tttccggccc
    17221 gacctcgagc ggcaagacct cgaccctcta tgccaccctg cacgaaatca actccgtgga
    17281 gaagaacatc atcacggtcg aagacccggt cgaatactcc ctgccgctca tcatccagat
    17341 ccagatcaac gagaaggccg ggctcacctt tccgacctgc ctgcgggcga tcctgcgcca
    17401 gaatcccgac gtcatcatga tcggcgagat ccgcgacccg gagacggctc ggatggccat
    17461 tcgctcggcg ctgacggggc acacggtgtt ctcgacaatc cacaccaacg acgccccctc
    17521 ggccatcgcc cgcctcgtcg acatggacat cgagcgctat ctggtggcct cggcgctcaa
    17581 gggcgtgctg gcccagcggc tcgtgcgcgt cacctgcccc gaatgcgcgg ccccctatcg
    17641 gccggcggag tcggtgctgc tgcaggccgg gctgcgcgac cgggacagtc agttcgattt
    17701 ccgcaagggc gtcgggtgcc cgaagtgcaa gaacaccggg ttccggggcc tcaccggcat
    17761 ttacgagtat gtcgaggtga ctccgacgat cgccgacatg ctcatgcgca acgcgcccct
    17821 caacgagatc cgggagtcgg cccgccgcca gggttacgtg cccctgttcg aggccggtct
    17881 ggaggccgtg cgggctggcc gcgtctgcct tgaggaactg ctcaaagaga cctcgaacgc
    17941 cgaggattac ctccggccgg gggagagcgc gtcgcggaga gccgtccatg cagagccggt
    18001 ataagtatcg cgcggccgac gcggcggggc ggacccgcga gggagtgctc ctggctgaca
    18061 gccgcgagca ggtgctggag catctggccg ggcagcagct ggtgccgatc cgcgtggccg
    18121 cagtcgccgg ccggcgtgga ttttcgctcc tcagcttctt ctccgggccg cactacgagc
    18181 gcctgattgc gtttacggcc aatctgggca cgatgtaccg ggcgggcgtg ccgctgctcc
    18241 gggccctggc cctgatccgc atcgggcctg ccgccgggca cttcaaccgc gcgatcgacg
    18301 acatccgcct ggccgtccag gccggccgcc cgctctcaca ggcgatggcg gcccacgacg
    18361 acctcttcgc gcgcgcctac gtcgccggcg tcgccgccgg cgaggagtcc ggcaagctcg
    18421 acgaaatcct cgcccaattg cgcacgatgc tcgaacagga cctcgagctc tcgcgcgcgg
    18481 tcaaggcggg cacccggtat ccgctgatgg tggtgggggc gctgggtgcc gccgccctcg
    18541 tgctcgtgct gtacgtggtc ccccggttca ccgctttcta tgggcagttc aacgcgcagc
    18601 tgccgctgcc gacgcgcttg ctgatcgggc tccagcatac ggtctccgcc tactggccgg
    18661 ccctgctgat ggcgctcgtg ctcgccgccc ttgcagtgcg ccaggcaatc ggaaccgaac
    18721 ggggcaagtc gtggctcgac caccgccttc tgcgtctgcc gatcgtcggt ccgctgatca
    18781 tcaagagcaa cgtcgcccgg ttcgccgcga tgttccatgt gctctttcag gccggcctcc
    18841 ccatcatcgc ctgcctcgaa attctggccg aaacggtcaa gaacagcgcg ctggccggag
    18901 aaatccgcac catggcgggt ctcttccgcg agggccgcga cagccgtctg gccgagcacg
    18961 agttccggta cttcccgccc ctggcgcttc agatgatgac catcgggctg gagtccggct
    19021 ccctcgccga ggttctccgc gaggtcggcg agcactacgg caaggaggtg cagtacacct
    19081 cccgccagct caccgctgtg ctcgagccta ttctcacgtt tgtcatgggc attttcgtgc
    19141 tcattctggc gctggcgatc ttcctgccaa tgtggaacct gatccgggtc ttcaacggct
    19201 gaggcgggct aaaaagggcg ggatattatt ccgataacga tagaagagta agtgtccaat
    19261 aatcagcccc ctgcaggagg tagattccat gaaccaactc cgcaaccggc gcggcttcac
    19321 gctgatcgag ctcgtcatca tcatcgtgat cctcggcatc ctcgccgccg tcgccatccc
    19381 gaaataccag gatctttcgg gcgaagcccg cgaggcgtcg gcccgcagtg ccctcggctc
    19441 gatgcggtcc gcggtcacca tcttctacgc caaccaggcc gtccagaccg gcaccgccac
    19501 ctggccctcg ctggccgatc tgcagacccg cggcacggtc atggcgcagg gcctgccgcc
    19561 gaacccgtat cagtccaatg cacccgactc ggtcgtcgac ggcaccggcc aggtgcgcgg
    19621 cactgtcatc ggcaaccgcg gcgggtgggt ttatgacccg gtctccggcg agctctgggc
    19681 caacaccgac acccacgacg aaaatgagtg gtgagcgcat ccggcccgac cccgcccgct
    19741 ccgatcgacg gctcagtgtt catccagccg gcttcacgct ggtcgaactg gtcataatca
    19801 tcgtcatcct cgggattctc gccgccgtgg ccatcccgca gctcgccgac atgagcggga
    19861 gcgctaaaac ggcggccacc cgaaaggaaa tgggcgagct gcggcgggcg ctggtcggct
    19921 cgcctgagat tgtcgccggc ggccggccgg tggccgctgg cttccaggga gatgtcggcc
    19981 ggttgcccgg acggctgagt gatctaacgg tccggcccga ctcggtcccg ccgtacgatc
    20041 ggctcgcccg gtacggctgg aacggcccgt acatcgaccc ctccggcgga aattacctca
    20101 gcgatgcctg gggcgtaccg tatgtgtacg acccggacgc ccggatcctc ctctccgtcg
    20161 ggggcggaga cactcttcgc gtgacgttgt gacaccgcta tgcgctcgct actgaagaag
    20221 ttcaccgccg gcgcacacgc gccctcctcc ccggcctcta ccggcccggc ggaacgcccc
    20281 cttgcgcccc gcggccgctt tctgatcggg cgtacgctcg ccgggcgggt cagcgatgat
    20341 gccctgtccc tggcggctgt ccgccacttc ggtgcccggc ggattgttac cgacgtcctt
    20401 catgcggatc tcccgccggg cgcacttccc gaggccggtt ccgagttcgc aatctcgcag
    20461 gtcgccgact tctggagccg gcacggcggg cgcggcgccc gcttcgcagc ctgcgtcgcc
    20521 ggggacggcg tcacggtccg gacgttctgc ctgccccgaa tgagtcgggc cgatctggcc
    20581 tcagccgtcg agattgaggc cagccgccag atccctttcc cggtcggcga gttcacctgc
    20641 ggttttcgcc cggtggcgcg actcgagggc gagggcgggg gccggtgcag ggtggcgctg
    20701 cttgcggcca cgcaccggct cctccgccag gcactcgttc ccctggaaca ggccgcggtt
    20761 ccggttcagg ccgtgtatgc cggagcgcaa gcggtcggcg tgctcctgac cgcgctgccg
    20821 aattttgacc ccgaccgcat ctacacgctg gtgcgtctcg gactgcgcga gagtgaaaca
    20881 gccttctacc gggccggcaa tctcgaattc tcccaccacg gcggcaccgg cacggcgatg
    20941 ctggggacgg cgggcgacga ggcgcggcgg gagttcttcg ccgaaacgct ggcctcggag
    21001 atccagacgt cgctcgatta tttcgcgggc cagcacctgc gcctccacga gccggaggtg
    21061 tttgtttacg gcggcgcggc cggccacacc cgcctgctga ggctgttgtc cgagcggctg
    21121 ggatgccccg tggagccgtt tccgctcgac cgtctcgcgg ggatccgcct ggccggccat
    21181 atcgaacgcg acgtcgcact ggccgctctg ccggcgatcg cggccgccct ctgccgctcc
    21241 gacctggtca acctcctgcc cccggatcgc cgggccgagt ggcgccggcg gcgggtcgat
    21301 gccctcggcc ggaccgccgt cgccgccgtc ctgcttttcc tggccgcggg cgggggattc
    21361 ctctaccacg atctccacgt gcagcaggag accgacctcg ccctccggca gcaattggct
    21421 ctctatgaaa actcgggagc gtaccgcgcg ctgagcagcc tgcggacccg tttggccgcc
    21481 gccgactcct acctcaaggc gagcgcgaag aatccgacct cgctggccct caatctaaaa
    21541 gagctttcgc atctgacgcc gcccgaagtg cggctgacgc gctacgactt cacccccgcc
    21601 gccgcggggg ccaacctccg cctgtccggc gtcgtcacat cttccgatgt gcctcccgag
    21661 gtagttttgg cggccttcgt cgatcggctg aacgcgggcc gcttcttcga cagcgtgacg
    21721 gtcgaacgcc acgtcaagcg ccgtcaggac gaaacattca tcctcgaatt tcagctcgcg
    21781 ctgcgggggc gcctgtgaaa ccgcgggtcc tgatatacgg aggaattgcc ctggccctgg
    21841 tggtgctgtg ggctttcctg gtgtacacgc cctaccgtca caaggcggcc tcggcccggg
    21901 ccgcggtgag agacaccgaa cgccggatag acgaggcgca ccggctcacc ctgcagcttc
    21961 cggcgtttct caaggccgag cagaccgccg agtcgctccg cctcgatctt gacgcccggc
    22021 tctacgcgca cgccgatgtg ctcgcgctgg tcgacgatct ggtggcgcgc gccgaccggc
    22081 accgcctgcg ggtggcggaa atcaccccgc ccgtcgagga gctgttgcag cttgatcgca
    22141 ccgccgccgc cgccagcccg ccgtttctca acctcgagat cacggtgacg ggggagttcg
    22201 agcagctcgg ccggttcgtg gccgacgtgg aggggagcga ttacgcccgg ggtatcaacc
    22261 ggtgcatcat tgccggcgct cccgacggtt ccgcgccgct ctccctgcag ctcggtcttc
    22321 gggcgctgct gggctcgagg ggaggcagcg catgagcccc gcccgccgcc gcctgtttct
    22381 gtacgtcctg ctcggcctgg cggtcgtgtg gggtgcctac agttttcgca ggccgcgccc
    22441 gatcgcgacg ccgccgccgg cgaccggcgc tgccgcggac actgcggttg tcccgcgctc
    22501 cgatccgccg gcgcgtcaga gcgacgtgtc ctcccttgcc gcgcggccct ggggggagga
    22561 tcccttccgg gtgcgggttc gccgcaccga tcgggaaccc gccgcccccg ccccgtcctg
    22621 gcggctgtcc ggcattgtgt actccgagaa tcacccgctg gcgattatca acagccggcc
    22681 cgtcggggtc ggcgaccgtg tggacggcgc cgtggtgatc gatatcaaca agcggcaggt
    22741 gacgctggag gccgacggtc ggcgcttcca gatcaacgtc gccgccaaag gatagccata
    22801 tgcttacgcg cacgatgttt gtctgtcttt gcctgggggc cgcgccggcg gccggtgcgg
    22861 aggtcacgcc cgactctgcc caactgctca ccctcagcct tgaaaacgtc gccgttgccg
    22921 acgtcctcaa cctgatcgcc gtccagaacg gcctcaacct tgtcgtttcc ggcgaggtca
    22981 ccgggaccgt gtcgctccgg ctggaggagg tatcctggac cagcgccctg gacgctatcc
    23041 tgctgccgat gggctacaat tatttcgtgc gcggcgaagt tatcatcgtc aaaccgttcg
    23101 accaggtctc ccccggcgag cttgacaccc gcaccgtgac cctgcgcttt ctggatccgg
    23161 tgacggcgaa gaaggcgctc gaacccctga aatcggcgca ggggcagatc attgtcctgg
    23221 acaaatcccc ctcggctgaa gcggtcgcgc tcccggcggc cgagacctac cggcccaacc
    23281 gcctcctcct ggccgatttc ccgtcggtcg tggaccgcat gctggccgtg ctcgaggaaa
    23341 tcgatcagcc cgaaccggtc gtcaccattg aagcccgcat catcgaaacc aacgtcgacg
    23401 acgacaccag gatcggcttc acctggccga ccgtgctgac cgccaccctc ggcggcgatt
    23461 cggcctcgac cggcgggtcc ggtctgtcgg gttcctcgct cgcctgggat cccaacaacg
    23521 gcgacctctc ctgggcgcgg ctctccgccg agcaggtcag cgtcatgctc cacctgctcg
    23581 agcagggcgg caacagccgt ctcatttccg atccgcacgt caccaccctg gccaatcacg
    23641 aggccgagat caagatccaa accgtcattc ccatcgccac catcaaccgc ttcaccgaag
    23701 gggcggtgat tcaggatatc gtcactttcc aggacgagga ggtcggcatc tccctgcgcg
    23761 tcaccccgcg cgtcaaccgc gaggggatga tcacgatgga agtgatcccg gtcgttgagg
    23821 atatcatcga tttcacgggc ccggtggaca accagaaacc gatcaccacg gagcgctcgg
    23881 tgcggacgac ggtggctgtc gccgagggag aaacgcttgc cctgggcggg ctgcgcaaag
    23941 agagcgagct gaagcagaaa cagcgggtgc cgctgctcgg ccgcatcccg ctgctgggaa
    24001 atctcctctt tacctacacg tcgaacgaaa agaagacgac cgacctcatc atcttgataa
    24061 cgccgcgcgt ccttccgtaa gtggccgtgc gcggccgaca aagaaccgcc gcgcgcgcga
    24121 gaagagcaac tgaagggtga gcacgaagag gccgaggtcg cgcacgagcg tggccgcggc
    24181 ctcgtcgccg gcccccaccg aggccttgaa acagccgcac tggatgtcca gcccccgaat
    24241 cgccgccgag gccagcgccg ccccgaacat aaccgtcatc aggagcatcg accacaccgt
    24301 cccccgatac cagactccga gcaccaggca cacccccgcg accagttccc accagggcag
    24361 cacgatcgcc atcaggttga tcagactccc cggcaccatg tggtagtacc agatgctctt
    24421 ggcgaaatcc atcggattga tgatcttgta gtaggacgca tagatgaaca caccccccac
    24481 gaacagccgg atgagcagcg tcagatagtc gttgtcgatc aaccgtcgca tttcgccctc
    24541 cgttccattt ccagcccgtt cttctcccac tcccgcgagc cgccgaaaaa aatcagcagg
    24601 tccgtgtaac cgaccgcctg caggttgcgg gccaggtgca gcgagaggtc gcactcctcc
    24661 cccgagcaga acgtgatcag ccgggtgtcc ctgggcgctc cggccagggc ggaatcgaaa
    24721 tacggggtga gatccccctc ggggagttcg tcaaacggga tattgaccgc gcccgggatt
    24781 gtcccgcagg cgaattcctc cggcgaccgc gcatcgatga aaatcacgcc gccgccggta
    24841 tactcgacat gggcgacatc gatcccgata tacgggggat cgcccggcgc atgctccggc
    24901 ggcttgatcg gcccgtcgcc cgatgacaac tcccgccatg tcccgatgta cgggatagcg
    24961 tggggcgaga ccagattggc cgccagacca agaaccgccg agagggtgac gatgaagata
    25021 agttgtcgta acgccatgct gtctgctcgc ttgcgagtcg gattcctttg gtcggttata
    25081 cagaccagcg gtccgaaggt ttggcctttc ggacgactac gagatcggcg ccttcggccg
    25141 ctttggcgga aacaacgctc ccccggttct caagtcaagt tgaaacgcgc cctggcagcg
    25201 tcatccattc cgcgcgcctc ggcggttcct ctccgggcat ctgatttgac ttgactcagc
    25261 cgcggcggcc tcgtatcttt cagtcaaagt cataaacctg actgacgaaa cgcagggcgg
    25321 tgtcggggac agcctcgaga ccacttcttc tcgccacaag acggtccgcc caaccgacgt
    25381 cgagccaatt gcccggactt ccgccgttac ggcatagtcg cggaggccgc tgtggctgcc
    25441 atgtcctggt cccggtcctt gggcggagcg cagggtgtgc accggatctc gtgcgtgcgc
    25501 ttttgccttc ggagagcaat ccccgcttcg tcgcccggcc cttccccccg gtgctccaca
    25561 tctcccaacc agagacgggt gttctgcagc gcgggagaga cgagacctgg ggccgaagcg
    25621 caggcttcaa tgatcatgcc gaaataccgc agtccgaccc acaatcaaac cgggccgctt
    25681 ggcccaacaa cgcaggaggc ctctatgaag gcaacggtgt tatggatctt catcatgctc
    25741 gccgcagtct tactcagcgg cacgaccggt tacacgcagg tgcccggcac catcaactac
    25801 caggggagac tcaccgggac cggcggcgcc ccccttgaca ccacggtcaa catggttttc
    25861 accatctaca cggcctcgag cggaggtacg gccttgtgga ccgagactcg cacgggagta
    25921 gtcgtcgccg acgggctgtt cagcgtcgcc ctcggatcgg tgaaccccat caacctctcg
    25981 gtctttggcg gggccgaccg ctggctgggg atcaaggtcg ggacggacgc ggagatgacc
    26041 ccccgggctc tgctctcagc cgctccctac gcccaggtgg cgtcgtgggc caagacctcg
    26101 gacacggccg attacgccat ggcgggcgcg gccagcggct ggactgacaa tggtgcgaat
    26161 gtcgtcctgc aatcctcgag cgataacgtc ggcatcggca ccaatgcgcc gcagtacaaa
    26221 ctgcacgttg cgggaaccat tctgatggat aacgctgtcg accaaggcgt ggaactgaga
    26281 tttgccgaaa acggactccg caaatggggt attttctatg caccgagcga tgggcgcgat
    26341 ctcagcatct acgatgacat gggtaaccgc tacctgatga ctttcgactc cggtaccggt
    26401 tttgtcggta tcggcaccag gaccccgact gtgccactag acgtagccgg taaaatcaag
    26461 tgcagcgatg acctctcagc caccgagaga atcttcatcg atggcgtcgg gggggacaac
    26521 gctgagatgc ggctcttgga agatggggta ggcaaatggg gcatgttctt ccgtccgagc
    26581 gatcaccgga gactaagcat ttacgatgac atggccaatc gctacgtcat gacgttcgac
    26641 tccggcaccg gcttcgtggg catcggcaca ggcactcccg ttgccaccct cgacgtcaac
    26701 ggacgtgccc gggtggcctc gtctcttctc gtcggggata ccatgacctg cgatatcgtc
    26761 aagatcaccg gcggcgccga tctcgcggag ccgtttgatc tgcacaacgc cgaaaccatc
    26821 cagccgggca tgctgctggt cattgacccg gccaaccccg gcaaactcac ggtcgcaaat
    26881 gcgccttatg atcactgcgt tgccggcgtc gtgagcggcg cagggggtat cgcccctggc
    26941 ctggtgatgg ggcaagccga gacgattgcc tcgggcaagt atccggtggc gatgaccggg
    27001 cgggtgtatt gtttggcgga cgccgacagg gcgccgattc gccccgggga tcttctcacg
    27061 acctcggcgg tgccgggcca tgccatgcgg gtcaccgatt tcgaccgagc ccagggagca
    27121 gtgatcggca aggcgatgac ggccctcgag agcggccgcg gcctcgtcct cgtgctcgtg
    27181 agcctgcagt aggcgaggag aaaaagcaat gactatcacg aaacagtcaa tctgcaaggc
    27241 tcctcccctg ccatggctcc ttatcgcgct ggcgctgacg gtgaccgcgg tgaccaccag
    27301 ttggagtgtg ccggcggagg atgtccagcg cgcggcggaa acctgggtgc ggtatgtgac
    27361 ggcccagccc aagccggacg cgatggtgat ccggatggaa ccgtacgagg cggagggcga
    27421 ggttgtcgca tatatcgcgc acctggcggg cggcggttac tgcctctgct ccaatgatac
    27481 gctgttgctg ccgatctacc tgtacaatcc gtcgggggag tacgatctgg aagtgccggc
    27541 ggtgcagtgt atcctcgaag gtattgcggc caaacaccgg acaatcaaga cgggggtcga
    27601 gcgcgatgac ccggtggtga tcgagcgcct ccccgcactg gtccagcgcg tgtccgcgtg
    27661 gcgctcgctg atcgccggac aggtaccctt ggggtacggg gtctatgacg atctgcgggc
    27721 tgacccggat tctatgcgcc tgccgctcac gtgcaagtgg gaccagccct cgccttacaa
    27781 cgactttgcg ccggtccttc accccggaac cgattcgcac tgtgtggccg gttgcgctcc
    27841 gatttcggca gctcaactgc tgtactactg gcagtggccg accaccggaa gcgggagcaa
    27901 aacgaagtac tacccgcggc gctattctcc cacgtgggtg gaacgaccct atcccaattc
    27961 cattactgtt ccggcaggaa tcctccataa ccggtttgag tggagttcag ccggcggagg
    28021 gaagatccgc ggatggggtt actgggactt ctacgtcaag gacaaggcat ccgaactcag
    28081 ttccgatccg gcgtaccaag cggcgcttga tgccatgttc tatgccatcc cggcggactc
    28141 ggtaccggtt tcggccaact ttggcgggac cacgtacgat tgggcgctca tggaggatgt
    28201 ccacaccgat gaagtagccg atcccggtga caccgccgcc gcctggttgt gccgagacgt
    28261 cgctatcgcc ataagcgcgc gctacggcta cggcgggacg tcaggggctg ttagcccgga
    28321 ctcactgcat aagtacttct tgtatggaac cgacggccat cacttgcctg tcaatgcgga
    28381 cacggcgaag gccgagattc gctggtttcg cccggtggtg gctgaaggat gcagccattc
    28441 gtggctcgtc tatggctact ataacgcaac cggcgagttc ctccataatc tcgggtgggg
    28501 tcctgacaac agcgccgctc tgggaccaat cgattcaatc gtgccctgca cgacgggggg
    28561 cgactggatg gcgaccatga ttgcccccga agactatgtc cggtttgtcg gcaacacgat
    28621 tgccggcgac gggtcgccga attcgcccca cctgaatatc gatgcagcgc tggcatcggc
    28681 cccggacggc gcgactcttg tcttcaaagc cgggacgaca cacatatccg cagcggctga
    28741 cctaatcatc tcgcgtccgc tgaccctgaa gggttacaat gtcattatca agggcgcggc
    28801 gaaatcgccc gggctcggaa agggggaacc cgaatgaaac ggccaatcgg aaactggtgg
    28861 tgggtgctcg gggcggcgct cctgctgatg cccctcgcgg gcttcatgca gccgaccgcg
    28921 acgccgccgg ccccggacgc gtcaagcgca cctctgcgcg acgccgatgc cgtcgccatc
    28981 aactggcagg tcgtggcttc cggtgggggg ccgggcgtct cggccaactt cctgctaaac
    29041 gggacagtcg gccagac
//
LOCUS       DAOPOE010000034        28986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2084_length_28986_cov_5.632643, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000034 DAOPOE010000000
VERSION     DAOPOE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28986
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2084_length_28986_cov_5.632643"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKM94_06280"
     CDS             complement(<1..906)
                     /locus_tag="PKM94_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD66286.1"
                     /translation="MAKDTILLVDDDPLVLEALRDLFSDDYTPLSARSGEEAVRIVEG
                     HRDICVVVMDIRMPGMDGIAAARAIARMEPELAVIFHTGYPGDYDEHEIDEREAPYDY
                     VEKGVSGLRLLRSVRHAAERSRLKGDVERLRGHAADCLGILGRSAGMLEVFRLIHRAG
                     PSNSKVMILGETGTGKELVAKALHALSPRAGKPLAVFNCNHKSTDLVESELFGHLKGA
                     FTGAIADRIGLFRYADGGTVFLDEIGDLDITTQAKLLRVLESGEFHPIGSPRIQACDV
                     RVLCATHRDLAGMVRESKFREDLYYR"
     gene            complement(948..3203)
                     /locus_tag="PKM94_06285"
     CDS             complement(948..3203)
                     /locus_tag="PKM94_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD66287.1"
                     /translation="MSSFLCVLFLLVNPPPAMGAASVSHFAVPVTRDVRLLPYRVDLI
                     FSGGVSPGAPLVCHPWRVFSAAESASDSVVILATSNQPESSDLPSMVELLRYPERLPL
                     EEHPIWLTVTDAGVWERNGEPPKGLWFAGYAQDSAWVCLVNGAAEPGGRLFLAAGTDS
                     TGDAVWRCALQEVGAFDYDFDGAVEHFFYLDPERGLRPRYLYCVEPAARRIEWGARVS
                     SPLPLGCIVPCGDSADAGILAITYAPGQGASDSLFSDSFGYLVRLDRAGAVRYRKIVS
                     RYSEATGLVPAADASGWYILHTLPLVDSVASGDLRPGPSVISRIDRDFRVVKSIAMPD
                     RVQYIFPADYDRDGAADLYAVSHEGVIRIFGADLTLKAESDSSYLQYRGQMTGWPGAD
                     TVLFFQERDTGGVYTPQFEKLAAVPTSDYRDVFAYDDRRRVAAILGADRDRHFIGYVR
                     KSTFMEAATAFYVDYQAWVLAALSSLLVGVAVLGAFRHRLSRRKQELESAHRKLAQAY
                     ADLRAAQEKIVAQEKYRQAKDIAGGFAHEIKNALFPVDGALLRQEKLLREPAPDSARL
                     RSTHATIRSAITRAVNLTRQITEYSRLEAEIRPEPADVRAILGEVLAANAAPVEERAI
                     GVAAEGEAAAVAAVRAGHLRSAFTNLLMNSMEALEGRDTPRITIAWTVRDRRILLEWT
                     DNGCGIAESDLPRIFDAFFSTKPTTGTGLGLSLVKRIVELYGGSIAAESRPGKGTAFH
                     LVLPMIPEQSG"
     gene            complement(3410..3760)
                     /locus_tag="PKM94_06290"
     CDS             complement(3410..3760)
                     /locus_tag="PKM94_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD66288.1"
                     /translation="MAAKRTKYFFQASNQQVDLVARAFRALGDPTRLKMLKALESGEQ
                     SVQDLVAQFTCSQPNISRHLSILTAAGLVAKNKQGPYVLYRVADPRIFELCDLVCGHV
                     YSLLARYSGKASSA"
     gene            complement(3889..7182)
                     /locus_tag="PKM94_06295"
     CDS             complement(3889..7182)
                     /locus_tag="PKM94_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD66289.1"
                     /translation="MTRHDTAGRGGEGLAGRLARMFVDSKLTPLFIIASILLGLFAVS
                     LLPREEEPQIKVPMIDVMVGVPGFSAEEVEKRVAAPMEKLIWEIPGVEYLYSISNPEQ
                     ALVIVRFRVGEDVEKSLVKLNQKLQSNFDRIPPGVSFPLLKPRSIDDVAILALTFHSP
                     TEDHYFLRRVVAEIEEQIKQVPNVSETHMIGGLRRQYRVQLDPDALAARNLDPLSVIP
                     ALQAANRQMRAGGIVRNNREVIVETGGFLRDVEDINRVVIGVFNGAPVYVRDVATVVD
                     GPEELTQVVVYGSAEASGRRHDDEAAVTLSVAKRAGTNAIEVANAVLEKVEHIRPRLV
                     PSDVQMTITRHYGETAEEKSNELLLHMLIAIVSVSILILISLGWRESIIVALAIPSTL
                     ALTLLVFFLTGFTLNRITLFALIFSIGILVDDAIVVVENITRHLHLPRNQRRPWKTIA
                     IEAVSEVGNPTVLATWAVIAAVLPMATVGGLMGPYMRPIPVGATAAMLFSLFVAFIVT
                     PWAAVRVLAWKRGAGHTAGHHPEGKEDRATRLYRRWMGRLVASPARSTVFFAVVGLLL
                     VGSIGLVGLGWVKVKMLPFDNKNEFQVIINMPEGSSLERTVEAAREIAQAVSREPEVT
                     NYQIYAGTSSPFNFNGLVRHYFLRRGEHVADIQVNLLPKGERSAQSHDIATRVRPRVQ
                     EIARRFAARVAVAEVPPGPPVLQTLVAEIYGPTDSSRTMLAHAVKAIFDSTPGVVDVD
                     TYIEDDQRKLTFEIDHEKAALHGISADQIARTLRVAVSGMQVDIAHRRHEREDIALVL
                     ELPRARKTSAADVLNLRVQSPRTGVLVPLAELVTVREAIVDKTIYRKNLMPVTYVIGD
                     VAGAIESPVYAIGEMNRKIAALNARDFGGTLPALKVYNASMPFTDLEPSLKWDGEWHI
                     TLEVFRDLGIAFAAVLVLIYVLLVGWFRSMVTPIILMVVIPLSLIGILPAHGAMDAFF
                     TATSMIGFMAGAGIVIRNSIILIDFAELRLREGSTLAEAVIDAGAVRFRPILLTAGAV
                     VVGAGIILFDPIFQGLAIALIAGEVASLVISPVAVPLLYYLVNRTKAAAGGGSESQTA
                     PTEGAPETL"
     gene            complement(7179..8210)
                     /locus_tag="PKM94_06300"
     CDS             complement(7179..8210)
                     /locus_tag="PKM94_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66290.1"
                     /translation="MKTIHYFSALVALAALAGCSTPGDRTPARAAASPKPVSVAKVER
                     LADQAADEIMGTVVARNTADIGSKVQARVERVLVDIGSRVNKGAVLAELDAREFEAKV
                     RQAQAMHEQAAQDLERFEKLLAQNAATRQEYDGIKARAAVARAALDEAETFLSYTRIT
                     APFAGAVTRKMIDVGDLTAPGMPTFTLEESGPPRFEATIPESYRRRIAAGQSVRVSIP
                     VIDTAVTGRVVDISPSADPMSRTFTAKIELPALADLRPGQFGRLQLQAEGDDATLAVP
                     RSACVHRGQLDLVYVVTPDKRAMLRLIRIGRQFPDRVEILSGLEVGETVVVSDQRDLL
                     DGDVVEEVS"
     gene            complement(8213..9517)
                     /locus_tag="PKM94_06305"
     CDS             complement(8213..9517)
                     /locus_tag="PKM94_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD66291.1"
                     /translation="MKLVVTVLTALVVAVRPAAAGELTLEQAVGLAREQSPQARIAAL
                     GVSEAAARAAEARAAFLPMVRVASGYTASDNAVNAFMFALNQGQFALAGDLNNPPRWD
                     NFQLSAQVGLNLFNGGRDLANLRAARAARAGAEFARSAADQEMVLGVTRMYLAVLTAQ
                     EAEEAARLAVEAYASAEQVMASRVENGTALRTDLLGIQVEKARAEERLLQARNGLALA
                     KEGLRLAIGLDTLGFDEFQSLDEIAPAAPAAVQAGVRPEVQAQTSFARAAFHEYRAAW
                     AGYLPSISAFAAVDYFRGLTYEHDNQSWTAGVMLNWTVFDGLFTGAAIREKRARLRAA
                     DEGARLARLQTSVELTSAAHGVEEAAGRVEVMQRAFDLAAEAVRLTEARFAQGLALTS
                     QVIDAQNNLAQTAVGLAQAKADRILALAQLRRALSVPILGER"
     gene            complement(9774..10682)
                     /locus_tag="PKM94_06310"
     CDS             complement(9774..10682)
                     /locus_tag="PKM94_06310"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOD66292.1"
                     /translation="MPEDADVLQLNGIFPPIPTAFDAEENLLPGRLQENLAHLSRFGL
                     AGFLVLGSNGEKVMLTDDEKRRVYEAARAAIPRDKLMLAGAGEEATRATVRMTRWAAE
                     AGADAVLVLNPGYYKGLMRREALLGHYRTVAESSPVPVVIYNMPACTGADLGAELVIE
                     LAAHPNIIGLKDSGGNITKMADIVRGTGPAFQVLAGSAGFLLPALSIGAAGGILALAN
                     IAPRECLAVYEHYRSGDPEKARAAQFRLIPLNAAVTSGGGVAALKAAMDHLGLYGGPA
                     RRPILPPGPDERKRLLALVDASSIAL"
     gene            complement(10798..11355)
                     /locus_tag="PKM94_06315"
     CDS             complement(10798..11355)
                     /locus_tag="PKM94_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66293.1"
                     /translation="MKSLRIFSAWVILLLGSALACSVAENRTAAPHSELPPVIDTLRG
                     LFDELQQAVLQGQPERFSALLDPAQADTLEKLIRKHGYLSLRSYIERQFARWPNLDTL
                     QLYEIKESGDYIRLTYAGPGTSFGAHRERVRYTFLLYKRIKGTWRLAAVTDFESDRYD
                     PYGYGCELSYHETDLPPRLRFPRLF"
     gene            complement(11492..12769)
                     /gene="clpX"
                     /locus_tag="PKM94_06320"
     CDS             complement(11492..12769)
                     /gene="clpX"
                     /locus_tag="PKM94_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOD66294.1"
                     /translation="MSADSEKPRPPQRCSFCGKPASQAKRLFSGYESYICNECVELCN
                     DLLQSAPDVALPEEIRDVPAPAEIKSYLDGYVIGQEKAKRTVAVAVYNHYKRINWELS
                     HRLNSSLAKDSDNVELEKSNILLLGPTGTGKTLIARTLAKFLKVPFTIADATVLTEAG
                     YVGEDVENILVRLYQAANFNQQKTERGIVYIDEIDKIARKDGNPSITRDVSGEGVQQG
                     LLKILEGTVANIPPKGGRKHPEQAFVQINTTNILFICGGAFDGLERIIARRLGKKQVG
                     FDAESTHIREDAPDLFEHVIPADLMEYGLIPEMVGRLPVTAALHALDEAALMNILTEP
                     KNALTKQYAKLLEMEGVNVVFETGALRAAVRIAQQRKTGARALRSIFERAMLDTMYSL
                     PSQSDVLEVIISEETITEGKPPRLRTKAEKKVG"
     gene            complement(12818..13435)
                     /gene="clpP"
                     /locus_tag="PKM94_06325"
     CDS             complement(12818..13435)
                     /gene="clpP"
                     /locus_tag="PKM94_06325"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOD66295.1"
                     /translation="MDEKIQAQYLVPMVVEQTGRGERAYDIFSRLLKDRIIFIGTPID
                     DNVANLVIAQMLFLEAEDPDKDIFVYLNSPGGSVTAGLAIYDTMQFIKPDVVTTCMGI
                     CASMGAFLLAAGTHGKRAALPNSRVMIHQPLAGTQGQISDIVIMTKEFTRTKERLNDL
                     LAQHTGQPLERIEKDTDRNFFMSASEAKDYGLIDKVYDFKRSERK"
     gene            complement(13484..14740)
                     /gene="tig"
                     /locus_tag="PKM94_06330"
     CDS             complement(13484..14740)
                     /gene="tig"
                     /locus_tag="PKM94_06330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOD66296.1"
                     /translation="MKVEIRELEELVREVSIEIEPAEVNKKLESKFSEVQRDVTLKGF
                     RKGHAPLNIIKSQFADEVKADVADELIKATISEALRERALPVATPPTVTDLNYTDNGG
                     FNYTARVEVFPTIPPIAFDNLKVSFYDLTVTDEEVDRAAEQVRKAYAERRTVTRAVGE
                     GDIVVVDLVQTFDSPKEEPEKAYPDSVIELSNPLTVKEFREQLPGLTAGDTREIEVHY
                     AADYPDEEFAGATVRYRTTVKEVREEIQPAFDDYLAKRTGHAQTALELRMKIREDLKA
                     QKERELRRYHRNEIVQQVIERNKVSVPAGLVKEYLDAMVKDLRRQDSTADEAELRGAY
                     HGMATAALQWNLLSDHIARQENIQVTREDTDKVIKRFAENYEITEAEAREGLRRSGKY
                     DNITDTLREEKVLDFLISKAEVVKKE"
     gene            complement(14910..14994)
                     /locus_tag="PKM94_06335"
     tRNA            complement(14910..14994)
                     /locus_tag="PKM94_06335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14958..14960),aa:Leu,seq:tag)
     gene            complement(15114..16316)
                     /locus_tag="PKM94_06340"
     CDS             complement(15114..16316)
                     /locus_tag="PKM94_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66297.1"
                     /translation="MSGSSETRAVWLIAGLLTGLSLLNHLISPSSFHLSAEAWDDLLA
                     YHRDFDVFRVRPVTTAVVGFLAEQTGLSVRSAFFLLQFALFFVTGPAFYHFLRSLQFS
                     PARSRAGMVILLLSLPLFLAHFEPVWTWDDFWCYILLPSAMAFALGRRYWPAAACIAG
                     AMIARETSAAFLPVLFLLFRTNPKVGIARPLLATLAPVAVLVVFRAAVLGAVSGSPVW
                     SLAFNFESWARTRDSLFSLIVSLGFLWPVGLTQAFRRPEESPAWYDAVRFGALLTAGA
                     YLVSGLLFGHIRESRLLLPPALLFIPLVLVYIEENGRRLGELRAVAAPGPLRTAIALG
                     LLLAGSLLAAHGLFPSFEYRRWQDGNRLYFALHLALTAVFLGVEFMRRGRGRSPVGRR
                     PGADARAA"
     gene            16606..17019
                     /locus_tag="PKM94_06345"
     CDS             16606..17019
                     /locus_tag="PKM94_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOD66298.1"
                     /translation="MHELGIAQSIVDKVLAKMTAEGYPEIVAIGLRIGALTDVVPDAL
                     TFGFESSVVGTPLARTRLAIETVPVAGVCETCGRTCAVEEFLFVCPHCGSRDIRMTQG
                     DELEIAYIEIPEPSPEIPGDKKPGGRESGRPAPQE"
     gene            17022..17693
                     /gene="hypB"
                     /locus_tag="PKM94_06350"
     CDS             17022..17693
                     /gene="hypB"
                     /locus_tag="PKM94_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOD66299.1"
                     /translation="MTERVEVKEKVLSENDRLAADLRRQLRDRRVFALNLVSSPGSGK
                     TSLLERTLAEFNDTIRIAVVAGDVQTENDANRLKAAGGRIVRPIVTGGLCHLDARMVT
                     KAVAEIDLAALDLLVVENVGNLVCPSSYDLGEDMKVVVISTTEGDDKPLKYPGMFRRS
                     SVMVVNKIDLLGSSDFTLAKVKENALSINGNLKIFEVSCRTGEGLDKWYQWLRDQVAA
                     SKASP"
     gene            17722..20022
                     /gene="hypF"
                     /locus_tag="PKM94_06355"
     CDS             17722..20022
                     /gene="hypF"
                     /locus_tag="PKM94_06355"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOD66300.1"
                     /translation="MLSAQDIQRQRLTVNGVVQGVGFRPFIYRLAHRLALAGSIRNDA
                     RGVHIEVEGPAAKLDQFRRLLRAEAPALARIDSLRVEPLPPRGQNGFSIEPSRHGETA
                     AALIPPDIAVCADCLREMHDPSDRRYRYPFTNCTNCGPRFTIVEGIPYDRPKTSMKRF
                     PMCPACEREYHDPLDRRFHAQPVACPACGPRLIAHDGARELAVADPVAWAVDLLASGR
                     ILALRGLGGFHLAVDAASEEAVRRLRDRKQRQQKPFALMAPDVARVRKFCEVSPEEEQ
                     LLTDVSRPIVLLRRRPGETAIAPSVVDDNMYYGFMLPYTPLHHLLLHDTFDALVMTSG
                     NRAEEPIVTENAEALARLGNIADGFLLHDRDILQRCDDSIVRRLAGGTRIVRRARGYV
                     PLSVPLPVRLERPILAVGGELKSTVALGRADQVVLSQHIGDLDNPAALAFFEHALSHL
                     QKLLECEPELIACDLHPEYLSTKWAHTQQALPVIGVQHHHAHLVSVLADNGCTEPAIG
                     LILDGSGYGTDGTIWGGEVLIGDAAAFRRFAWLEPVPLPGGTVAIEQPWRMALSYLSY
                     AYGGEARRFPLPLFDRLDSLQIDIILAMIDKGLNSPLTSSCGRLFDGVAALAGLALEN
                     TYEAQAAIALEMAATRAGGALPDVEPAAPPAGGGPLSFAHLIREVVRRVRSGDPVEQI
                     AAQFHAALAELFAGAALAARAATGLTTVALSGGVCQNALFFTRLAHRLENAGFRVLAH
                     REVPTNDGGLALGQAVIADAVHRRTS"
     gene            20032..20313
                     /locus_tag="PKM94_06360"
     CDS             20032..20313
                     /locus_tag="PKM94_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOD66301.1"
                     /translation="MCLAIPGKVIEIFDEDGLTMGRIDYAGTVRTACLDSVPEVRVGQ
                     YVLVHAGFALNVLDEDEARRTLDLFDEIARQNAEEGLDPFGEPLPPEAP"
     gene            20319..21416
                     /gene="hypD"
                     /locus_tag="PKM94_06365"
     CDS             20319..21416
                     /gene="hypD"
                     /locus_tag="PKM94_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOD66302.1"
                     /translation="MKYLDEFRDPRRAAALLGEIRRTVTRPWVIMEICGGQTHSIIRN
                     GLDQLLPPTIRLVHGPGCPVCVTSLELIDRALAIAARPDVIFASFGDMLRVPGSSKDL
                     LTVKSEGGDVRMVYSPLDALRLARAHRDRRVVFFAVGFETTAPATATAIRQARREGLD
                     NFLLLVAHVLVPPAMRALLEAPGNRVQAYLAAGHVCAVTGWEEYCPIAEGYRVPVVVT
                     GFEPADILDGILAAVRQLETGQARVENRYARAVREQGNPHARAAVEAVYEVCDRKWRG
                     IGTIPQSGYRLRPEFAAHDAERIFRLADLEVLEPPECISGLVLQGLRRPVDCPAFARR
                     CTPQTPLGATMVSSEGACAAYYRYHRRAAES"
     gene            21430..22494
                     /gene="hypE"
                     /locus_tag="PKM94_06370"
     CDS             21430..22494
                     /gene="hypE"
                     /locus_tag="PKM94_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOD66303.1"
                     /translation="MPDEPLPEPSFSCPLPLSAPATIQLAHGSGGRLANDLIARLFVE
                     AFDNPMLRRQDDQATVEIDGLRLSFSTDSFVVDPLFFPGGDIGDLAVNGTVNDLSMNG
                     ARPLFLSAGFIIEEGLPIADLARVVQSMRKAAAAAGVQIVTGDTKVVNRGKGDKLFIN
                     TAGIGVISHPYDIGVDRLRPGDRVLISGPLGNHGLAVLSRREGLAFESPIVSDTAPLN
                     GLVAALVAGVGGALHAMRDPTRGGLAATLNEFARASRVGIRLVGAQVPIDPPVAGACE
                     LLGLDPLYVANEGKLTAVVSAEAADRALALMRAHPLGARAAVIGEVHADRPGEVTLRT
                     VLGTERIVDMPVGEQLPRIC"
     gene            22596..23684
                     /locus_tag="PKM94_06375"
     CDS             22596..23684
                     /locus_tag="PKM94_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOD66304.1"
                     /translation="MSHLPPPAIMYRALRDRDATFEGVFYVGVRTTGIFCRPTCPARK
                     PKEENVQFFAFPREALQAGYRPCARCAPLEVERPAPALVRALRNLVDESPGGKISGAL
                     LRARGIDPSTARRQFQRYYGMTFHAYQRARRLGLALHEIRRGESVIGAQIDHGYESAS
                     GFFEAFRNAFGAPPSRLETVACLFAQAIETPLGPMVAVADDSGLHLLDFVDRGGWQQT
                     VGKLRERSATAVVPGPHPHLAAVAEQLKSYFEGRSLDFSVPLVPDGTPFELAVWSLLQ
                     TIPPGAAWSYAQMAARLGRPAAVRAVGRANSRNRLALVIPCHRVIRADGTLCGYGGGI
                     WRKQWLLAHERRVLSRRETSPVTPGPTG"
     gene            complement(23662..25104)
                     /locus_tag="PKM94_06380"
     CDS             complement(23662..25104)
                     /locus_tag="PKM94_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD66305.1"
                     /translation="MTTAVDTGKTRVAETERDEKLVNAFFVLYRNARIMEPANKAFVK
                     QCAHFLELLQPGLKADAEVAIKAVRGRYFTNERFVRFSDPSGAAAAVTADWEALGIGG
                     VRFLPEVTEDHLRGFFSFMAALTPRGRSLAELQEELDRHIPATVFLLALPDENEDDED
                     EAERRRQLRLQARTTFFRAVSAVSELLARTAVERDVNLAKTRRVVHALIDHLSKDKDS
                     LVELSAIKDFDDYTYAHSVNVSVYSLTLGIRLGLDRPRLSQLGFAALFHDAGKVRLDR
                     DLINKPDSFDENDWIQMQMHPALGAKTILRSMKLDQHTARAARGAFEHHINADYTGYP
                     LLHDIRPLNLYSQIITIADTFDALTSDRVYFRKAMAPDQVIRKMRFQMTAKFNPILIA
                     LFNEVMGIYPAGSMALLSTDEIAVIMAANAELYDRPYVQIVGNREGLLPEPEWVDLAR
                     ADQINRRIVRLIDPERYGLKVRDFILSGPE"
     gene            complement(25101..26945)
                     /locus_tag="PKM94_06385"
     CDS             complement(25101..26945)
                     /locus_tag="PKM94_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD66306.1"
                     /translation="METPNTDRPPDQEIAVLLRDLLRTIKAVCMYPETNSLPQSLRQS
                     FASRLLDLLEEQGEIRLGVDNGVILRNGEIVHRDKSREDNLAAIFFSAGITELSFKRG
                     LTAASIDRLLGAIRTYLTAPAGHGDLPSLLWEAAPEGFSFQSAEDVALAEYDAGFRVQ
                     EYFHREDERRRAGGDTPDGYEAIFLSEDALEGRAAPETVDGHLTPGGVFEVEPEEAAR
                     LRAQAAAEAMGLSNLPPVPVPSVRDILSRENSLSADEQARMEQLLAQDAAFNLYESTS
                     HLLIELLMQETTLKGFDETAIICEKMLNEFVAQGQVAHAAFLLRSLRNLGERIRGAKP
                     RWADRLEGIYMTAGSRERLRLLAKALNEHTEITESDLRRYLDLFDWQALSALTDLLGE
                     LEHRPHRETLCRYLVDHGRGKEEILAKGVYDKRWYVVRNSVMILGHIGDDRALQHLKQ
                     AVAHEDRRVRIELVEAVRGKEHAGALEILGRLVFDPDEEVHREAMAALMGSSGAGAFE
                     ALAGAVTAESFGQLDPQDQIALLVAYSRRGKERAVPHLARLIARFNPTRNPIRTFFRA
                     AAFDALEQNPSEKAGALLQRLTGSWRPDIRRRATRCLSRRKAPTGGEA"
     gene            complement(27075..28424)
                     /locus_tag="PKM94_06390"
     CDS             complement(27075..28424)
                     /locus_tag="PKM94_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD66307.1"
                     /translation="MAKKSAPKQSGVSGHRQLMEKITSKKAKAGVIGLGYVGLPLAVE
                     LVHAGFEVTGFDIAEPKVRLLNSGRSDIDDVPDAVVADMVAAKKFRATTDFRALGEMD
                     TISICVPTPLSKTKDPDVSFILAAVGSVKQFMRKGTLVVLESTTYPGTTEDLILPLLQ
                     EKGMKAGEDFFLAFSPERVDPGNQRFTTKNTPRVVGGITPKCTQVAKTFYEQSIPDIY
                     AVSSTQAAEMVKLLENTFRSVNIGLVNEVALMCDRLGLNVWEIIDAAATKPFGFMPFY
                     PGPGLGGHCIPIDPHYLAWKLKSLNYYARFIELAGDINSSMPHYVVDRVMRMLNERFA
                     KALNKSSVLVLGIAYKANIKDVRESPALDVIKLLEDRGARVVFNDPHVPSIQWEGTAR
                     RSTRLTAALLRKTDLVVIVTNHAQYDYQWIVDNAKAVFDTRNATRKVRNGRKKIEVL"
     gene            complement(28444..>28986)
                     /locus_tag="PKM94_06395"
     CDS             complement(28444..>28986)
                     /locus_tag="PKM94_06395"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD66308.1"
                     /translation="PTSPYGISKAAGEHLCRSHFRRYGLPVTVARSFNHSGPRQAPIF
                     VIADFARQAAAIDLGLQPPRVRVGNLAARRDFSDVRDIVRGYRLLAERGAPGEVYQLC
                     SGRATSIETVLRLLLGFTAKPVAIAVDRGKWRAADIPVLRGSNRRAVEELGFANRYSL
                     KQTLRETFAYWKERLSAENE"
BASE COUNT         4741 a        10115 c         9251 g         4879 t
ORIGIN      
        1 acgatagtac aggtcctcgc ggaatttgga ttctctcacc atgcccgcca ggtcgcggtg
       61 ggtcgcacag agcacccgca catcgcacgc ctgaatccgg ggcgagccga tgggatggaa
      121 ctccccggac tcgagaacgc ggagcaactt cgcctgcgtc gtgatgtcga gatcgccgat
      181 ttcatcgaga aaaaccgtgc cgccgtcggc gtatctgaac agcccgatgc ggtcggcgat
      241 cgccccggtg aaagcccctt tcagatggcc gaacagctcg gactccacga gatcggtcga
      301 cttgtgattg cagttgaaca cggccagcgg ttttccggcg cgcgggctca gcgcatggag
      361 ggccttggcc accagctctt tgcccgtgcc ggtctcgcct agaatcatga ccttgctgtt
      421 cgacgggccg gcccggtgaa tgagccggaa cacctccagc atgcccgccg accgccctag
      481 gatacccagg cagtcggcgg cgtgtccccg gagtcgctca acatctccct tcagccggct
      541 gcgctccgcc gcatgccgaa ccgatcggag gaggcgaagc ccggagactc ctttctcgac
      601 atagtcgtag ggtgcctcgc gctcatcgat ctcgtgctcg tcatagtcgc ccggatagcc
      661 ggtgtggaat atgaccgcca gttccggctc catgcgggcg attgcgcggg cggcggcgat
      721 cccgtccatc cccggcatgc ggatgtccat gaccacgacg cagatgtcgc ggtgcccctc
      781 cacgatgcgg acggcctcct cgccggaccg ggcgctcagc ggagtgtagt cgtcggagaa
      841 cagatcgcgc agggcctcga gcaccagcgg atcgtcatca accagaagaa tcgtgtcctt
      901 tgccatacct gtcgccgccc tgtggttgca ccagcctgtt ttctcgttta accggattgc
      961 tccggaatca tgggcaagac cagatgaaaa gccgttccct tccccggccg gctctcggcc
     1021 gcgatgcttc ccccatacaa ctcgactatc ctcttcacca gcgagaggcc cagccctgtc
     1081 ccggtcgtcg gcttggtcga gaagaaagcg tcgaagatcc gcggcaggtc ggattccgca
     1141 atcccgcacc cgttgtcggt ccactccaac aggatgcggc ggtcgcggac cgtccaggcg
     1201 atcgtaatgc ggggggtgtc ccggccctcg agcgcctcca tgctgttcat gaggagattg
     1261 gtgaaggcgc tccgcaggtg gccggcccgc acggccgcga ccgcggccgc ctcgccctcc
     1321 gcggccaccc cgatcgcccg ctcctcgacc ggggcggcgt tggccgcgag gacttccccg
     1381 agaatcgcgc ggacgtctgc cggttccggt cggatctctg cctcgagccg ggagtattcg
     1441 gtgatctggc gggtgaggtt taccgcccgc gtgatcgcac tgcggatcgt cgcgtgcgtc
     1501 gatctcagcc gcgcgctgtc cggggccggc tcccgaagga gcttctcctg ccggagcagg
     1561 gcgccatcca ccgggaagag cgcgttcttg atctcgtggg cgaaaccgcc agcaatgtct
     1621 ttcgcctgcc ggtacttctc ctgggcgacg atcttttcct gggccgccct gaggtccgca
     1681 tacgcctgcg cgagtttccg gtgggccgat tccagctcct gcttgcggcg cgacagcctg
     1741 tggcggaagg cgccgaggac ggcgaccccg accagcagcg acgagagcgc cgcgagtacc
     1801 cacgcctggt agtccacgta gaacgccgtc gccgcctcca tgaaggtgct cttcctcacg
     1861 tagccgatga agtggcggtc ccgatcggcg cccagaatgg cggccacccg acgccgatca
     1921 tcataggcga aaacatcgcg gtagtcgctc gtcgggaccg ccgccagttt ctcaaactgc
     1981 ggcgtgtaaa caccgccggt gtcccgctcc tgaaagaaga gcactgtgtc ggcgccgggc
     2041 cacccggtca tctgaccgcg atactggaga tagctggagt cggactcggc tttcaatgtc
     2101 agatccgcgc cgaagatccg tatcactccc tcgtgcgaca ccgcatagag gtccgccgcc
     2161 ccgtcgcggt cgtagtcggc cggaaagatg tactggacgc ggtcgggcat ggcgatgctc
     2221 ttgaccaccc gaaagtcgcg gtcaatccgc gagatcacac tgggccccgg ccggagatcg
     2281 cccgacgcca cgctgtcgac cagggggagc gtgtgcagga tataccagcc cgaggcatcg
     2341 gcggccggga ctaatcccgt cgcttccgaa tagcgcgaga cgattttccg ataccggact
     2401 gctcccgccc ggtcgaggcg gacgagatac ccgaaagagt cgctgaaaag cgaatccgag
     2461 gccccctggc ccggcgcgta ggtgatcgcg aggattcccg catcggcgct gtccccgcac
     2521 gggacgatac acccgagcgg cagcgggctg gatactcgcg cgccccactc gatccggcgg
     2581 gcggccggct ccacgcagta taagtaccgg ggtcggaggc cgcgttcggg atccaggtag
     2641 aagaagtgct cgaccgcgcc gtcaaagtca tagtcaaacg cccctacctc ctggagggcg
     2701 catcgccaca ccgcatcccc cgtcgagtcg gtacccgcgg ccaggaacaa ccggccgccg
     2761 ggctcggccg ctccgttcac caggcacacc caggcgctgt cctgcgcgta gcctgcaaac
     2821 cagagcccct tcggcggctc tccattgcgc tcccagaccc cggcatcggt gacggtcagc
     2881 cagatgggat gttcctccag ggggagccgt tccgggtagc gcagcagttc caccatcgac
     2941 ggcaagtcgc tcgattccgg ctggttggat gtcgcgagga tcaccacgga gtcgctcgcc
     3001 gactccgcgg cgctgaagac tctccagggg tggcacacca gcggggcgcc cgggctaacc
     3061 ccgccggaga aaatcaggtc gacccgatag ggcagcaggc gcacatcgcg cgtcaccgga
     3121 acggcgaaat gggagaccga tgccgcgccc atcgcggggg gaggattcac gagcaggaaa
     3181 agcacgcaca ggaaggaact cacgccagaa acctccgccg gacaggagac acttttctgc
     3241 tctcacccgg atgccgctgc cggagaaggg aaggatccgc agcacccgcc gggaagatac
     3301 aatactgcaa tatacgcaag cgggtcccga atggaaagac ccattgcgcc cgtccccgga
     3361 ggatcttggc cgagcctcgc cggaggccgc cactgcggtg gcggaccagc tacgccgagg
     3421 acgccttgcc gctgtaccgc gccagcaggc tgtagacgtg cccgcacacc aggtcgcaca
     3481 gttcgaaaat gcgcggatcc gccacccggt acaggacgta cggaccctgc ttgtttttgg
     3541 ccaccagccc ggccgcggtg aggattgaga ggtgccgcga aatgttgggc tgggagcaag
     3601 taaactgggc caccagatcc tgcaccgact gctcgcccga ttcgagcgcc ttgagcattt
     3661 tcagccgcgt cgggtcgccc agcgcccgga aggcgcgggc gaccagatcc acctgctggt
     3721 tgctcgcctg gaaaaagtac ttggttcgct tggctgccat gttccgggta ctcccgtctc
     3781 gtcttgattc cgtcactgca caatgccggg cgcgatcctg cctccgcctg ccgcccgccc
     3841 gggccgggcg ggtgggtttg gcttccggct ctgcgccggc cggtcgcgtc acagcgtctc
     3901 cggcgccccc tcggtcggcg ccgtctggga ttccgatcct ccgcccgccg ccgccttcgt
     3961 gcggttgacc aggtagtaca ggagcggcac ggcgaccggg ctgatgacga gcgaggcgac
     4021 ctcgccggcg atgagcgcga tggccaggcc ctggaagatc gggtcgaaga ggatgatgcc
     4081 ggcgcccacg accaccgcgc ccgccgtgag cagaatgggg cggaatcgca ccgcgccggc
     4141 gtcgatcacc gcctcggcca gggtgctgcc ctcacgcagg cgaagctcgg cgaagtcgat
     4201 caggataatt gaattgcgga tcacaatccc ggcccccgcc atgaaaccga tcatcgaggt
     4261 cgccgtgaaa aatgcatcca tcgccccgtg ggccggcaga atgccgatga gcgagagcgg
     4321 gatcaccacc atcagaatga tcggcgtgac catcgaccgg aaccatccca ccagcaggac
     4381 atagatgagc acgaggaccg cggcgaacgc gatgcccagg tcgcggaaca cctcgagcgt
     4441 gatgtgccac tcgccgtccc acttcagcga gggctcgagg tcggtgaacg gcatcgacgc
     4501 gttgtacacc ttcagcgccg ggagcgtccc cccgaaatcg cgggcgttga gggcggcgat
     4561 cttccggttc atctccccga tcgcgtagac gggggactcg atcgcgccgg cgacgtcgcc
     4621 gatgacgtag gtcaccggca tcaggttctt gcggtagatc gtcttgtcga cgatcgcctc
     4681 ccgcaccgtc acgagctcgg cgagcggcac cagcaccccg gtccggggcg actgcacgcg
     4741 gagattaaga acgtcggcgg cgctggtttt ccgcgcccgc ggcagctcga gcaccagggc
     4801 gatgtcctcg cgctcgtgcc ggcgatgcgc gatatccacc tgcatccccg acaccgccac
     4861 ccggagcgtc cgggcgatct ggtcggcgct gatgccgtgg agcgccgcct tctcgtggtc
     4921 gatctcaaac gtcagcttcc gctgatcgtc ctcgatgtac gtgtccacgt ccaccacgcc
     4981 cggcgtcgaa tcaaagatgg ccttgactgc gtgggcgagc atggtgcggc tggagtcggt
     5041 cggcccgtag atctccgcga ccagcgtctg cagcacgggc gggccgggcg gcacctcggc
     5101 caccgccacc cgggccgcga agcgccgggc gatttcctgc acccggggac gcacgcgggt
     5161 ggcgatgtcg tggctctgcg ccgagcgctc ccccttgggc agcagattca cctggatgtc
     5221 ggcgacgtgc tccccccgcc gcaggaaata gtgccgcact aggccgttga agttgaaggg
     5281 ggacgacgtc ccggcgtaaa tctggtaatt ggtgacctcc ggctcccggc tcaccgcctg
     5341 ggcaatctcg cgcgccgcct ccacggtgcg ctccagcgac gatccctcgg gcatgttgat
     5401 gatgacctgg aattcattct tgttgtcaaa cggcagcatc ttgaccttga cccatccgag
     5461 gccgaccagg ccgatcgaac cgaccagcag cagtcccacg acggcgaaga agacggtgct
     5521 gcgcgcgggc gaggccacca ggcgccccat ccaccgacgg tagagccgcg tcgcgcggtc
     5581 ctccttcccc tcggggtgat ggccggcggt gtggccggcc ccccgcttcc aggcgagcac
     5641 gcgcaccgcc gcccagggcg tgacgatgaa cgcgacaaag agcgaaaaca gcatcgccgc
     5701 cgtcgccccc accgggatcg gccgcatgta cggccccatc agcccgccca ccgtggccat
     5761 cggcagaacc gccgcaatca ccgcccaggt ggccaggacc gtgggattgc ccacctccga
     5821 gaccgcctcg atggcgatgg tcttccacgg ccgccgctgg ttcctgggaa ggtgcaggtg
     5881 ccgggtgatg ttctcgacca ccacgatcgc atcgtcgacc agaatgccga tcgagaagat
     5941 gagagcgaac agggtgatgc ggttgagtgt gaagccggtg aggaagaaca ccaggagggt
     6001 gagcgcgagg gtggacggaa tcgccagggc gacaatgatc gactcgcgcc agcccaggct
     6061 gatgaggatc agaatcgaca ccgacacgat cgcaatcagc atgtgcagca agagttcgtt
     6121 ggacttctcc tcggccgtct cgccgtagtg gcgcgtgatc gtcatctgca catccgaggg
     6181 cacaagccgc gggcggatgt gctccacctt ctcgagcacc gcgttggcca cctcgatcgc
     6241 gttcgtgccg gcccgcttgg ccaccgagag ggtgacggcg gcctcatcgt cgtgccgccg
     6301 ccccgacgcc tccgcggatc cgtacaccac aacctgggtg agttcctccg gaccgtcgac
     6361 caccgtcgcc acgtcgcgca cgtacacggg cgcgccgttg aagacgccga tgacgacccg
     6421 gttgatgtcc tcgacgtcgc gcaggaaccc gccggtctcg acgatgacct cgcggttgtt
     6481 gcggacgatg ccgcccgccc gcatctgccg gttggccgcc tggagcgccg ggatgacgct
     6541 gagcggatcg aggttgcggg ccgccagggc gtccgggtca agctgcaccc ggtactgccg
     6601 ccgcaggccg ccgatcatgt gcgtctccga cacgttcggc acctgcttga tctgctcttc
     6661 gatctccgcc accacgcggc ggaggaagta gtggtcctcg gtggggctgt ggaaggtgag
     6721 ggccaggatc gccacgtcgt caatcgaccg cggcttgagc agcgggaacg acacgcccgg
     6781 cgggatgcgg tcgaaattcg actgcagctt ctggttcagc ttgaccagcg atttctcgac
     6841 atcctccccg accctgaacc gcactatcac cagcgcctgc tccgggttgg agatggagta
     6901 gaggtattcg acgccgggaa tctcccagat cagcttctcc atcggagccg ccacccgctt
     6961 ctcgacttcc tccgcgctga aaccgggcac gccgaccatg acgtcgatca tcggcacctt
     7021 gatctgcggt tcctcctcgc gcggcaggag cgagacggcg aagaggccca gcagaatgga
     7081 ggctatgata aacagcggcg tgagcttcga gtcgacaaac atccgggcca ggcggcccgc
     7141 cagtccctcg ccgccgcggc cggcggtatc gtgacgcgtc atgagacctc ctcaacgacg
     7201 tcgccgtcga gcaggtcgcg ctggtcgctg accaccaccg tctcccccac ctccaggccg
     7261 gagaggatct ccacccggtc ggggaactgc cggccgatgc ggatgaggcg gagcatcgcc
     7321 cgcttgtccg gcgtgaccac gtacacgaga tccagctggc cgcggtggac gcaggcggac
     7381 cggggcaccg ccagcgtggc atcgtccccc tccgcctgca gctgcaggcg gccgaactgc
     7441 cccggccgca ggtcggccag cgcgggcaat tcaattttcg cggtaaacgt ccggctcatg
     7501 ggatcggccg agggggagat atccaccacg cggccggtga cggcggtgtc gatcaccggg
     7561 atcgagaccc gcaccgactg gccggcggcg atgcggcggc ggtacgattc cggaatagtc
     7621 gcctcgaacc gcggcggacc ggactcctcg agcgtgaatg tcggcatgcc aggcgcggtc
     7681 aggtcgccga cgtcgatcat cttgcgggtg acggctcctg cgaagggcgc ggtgatgcgc
     7741 gtgtagctga gaaaggtctc cgcttcgtcg agcgccgccc gggccaccgc cgcgcgcgcc
     7801 ttgatgccgt cgtactcctg ccgcgtcgcc gcgttctggg cgagcagctt ctcgaaccgc
     7861 tcgagatcct gcgccgcctg ctcgtgcatc gcctgcgcct gccgcacctt ggcctcgaat
     7921 tcgcgggcgt cgagctccgc cagcactgcc cccttgttga cgcggctgcc gatatcgacc
     7981 agcacccgct ccacgcgcgc ctggactttg gagccgatgt cggcggtgtt gcgggccacc
     8041 actgtcccca tgatctcatc ggccgcctgg tcggcgagcc gctccacttt ggccacggag
     8101 acgggcttgg ggctcgccgc cgcgcgcgcc ggcgtcctgt cgcccggggt gctgcagccg
     8161 gccagggccg ccagagccac gagcgcggaa aagtaatgga tcgtcttcat ggtcaccttt
     8221 ctcccagtat gggcacgctg agcgcccggc gcagttgcgc cagcgccagg attctatctg
     8281 ctttcgcctg ggccagaccg accgccgtct gcgccagatt gttctgagca tcgatcacct
     8341 gcgaggtcag ggcgagcccc tgcgcaaacc gcgcctccgt gagccggacc gcctccgcgg
     8401 ccagatcgaa tgcccgctgc atcacctcaa cccggcccgc cgcttcctcc acgccgtggg
     8461 cagccgaggt caattcgacc gaggtctgca gccgggccag acgcgccccc tcgtcggccg
     8521 cccgcaggcg ggcccgcttc tcccggatcg ccgcgcccgt gaacagaccg tcgaacaccg
     8581 tccagttcag catcaccccc gccgtccagc tctggttgtc atgctcataa gtgagcccgc
     8641 ggaaatagtc cacggcggca aaggcggaaa tggagggcag atatcccgcc caggccgccc
     8701 ggtactcgtg gaaggcggcg cgggcgaacg aggtctgcgc ctgcacttcc ggacgcacgc
     8761 cggcctggac cgcggccggc gcggccggcg caatctcatc gagcgactga aactcatcga
     8821 accccagcgt gtccagcccg atcgcgagcc ggagcccctc tttggccagt gcgagaccgt
     8881 tgcgcgcctg gaggagccgc tcctcggccc gcgccttctc cacctgaatg ccgagcagat
     8941 ccgtccgcag cgccgtgccg ttctccaccc ggctcgccat gacctgctcc gccgaggcgt
     9001 acgcctcgac cgccaagcgc gcagcctcct ccgcctcctg cgcagtcagc accgcgaggt
     9061 acatccgggt gactcccagc accatctcct gatcggcggc gctccgggcg aactcggcgc
     9121 ccgcccgggc cgcccgggcg gcccgaaggt tggccagatc gcggcccccg ttgaagaggt
     9181 tcaggccgac ctgggccgag agctggaaat tgtcccagcg cggcgggttg ttcagatcgc
     9241 cggccagcgc aaactgccct tgattgagtg cgaacatgaa ggcgttgacg gcattgtcgc
     9301 tggccgtgta gccggaggcc acccgcacca tcggcagaaa cgccgcccgc gcttcggccg
     9361 cccgcgcggc cgcctcgctc actccaagcg cggcgatccg ggcctgcggc gactgctccc
     9421 gcgccaggcc gaccgcctgc tccagcgtca actccccggc ggccgccggc cgcaccgcca
     9481 ccaccagggc ggtcagaact gtgaccacaa gcttcatacg actcctcgga ctctgtccat
     9541 tctgggcctc ccgggggcgg cgcctcccgg atccgccata tataacgata taaacatata
     9601 gttatatata tttgtgccga gaaaagtgca agggtttttt ccttttttgg gggctgtccc
     9661 gacagaaaaa accggcccgc cggctccccg ctcgaatcag gtcgggtcgg gagggccgct
     9721 gtcaggaaag tcgcgctgag ggagaaggcc cccggccggc cgcggcgccc gttttagagg
     9781 gcgatgctgc ttgcgtccac cagcgcgagc aggcgctttc gctcgtccgg gcccggcggg
     9841 agaatcggac ggcgggccgg tccgccgtag aggccgaggt gatccatggc cgctttgagc
     9901 gccgccaccc cgccgccgct agtgacggcc gcgttgagcg ggatgaggcg gaactgggcg
     9961 gcgcgcgcct tctccgggtc gccgctgcga tagtgctcgt agaccgccag gcactcgcgc
    10021 ggggcgatat tggccagagc caggataccg ccggccgccc cgatcgacag ggcgggcagc
    10081 aggaaaccgg ccgacccggc cagcacctgg aacgccggtc ccgtgccgcg cacgatgtcc
    10141 gccattttcg tgatgttccc ccccgagtcc ttgagcccga ttatgttggg gtgggccgcc
    10201 agctcgatga cgagctcggc gccgagatcc gcgccggtgc aggccggcat gttatagatg
    10261 acgaccggga cgggcgaact ctccgccacc gtccggtaat ggccgagcag cgcctcccgc
    10321 ctcatcagcc ccttgtagta gccgggattg agcaccagca cggcgtcggc gccggcctcg
    10381 gccgcccacc gcgtcatccg caccgtcgcc cgcgtcgcct cctcccccgc gccggcgagc
    10441 atgagtttgt cgcgcgggat ggcggcccgg gctgcctcgt acacccggcg cttctcgtcg
    10501 tcggtcagca tcaccttctc gccgttcgac ccgagcacga gaaagccggc cagaccgaac
    10561 cggctcaggt gcgcgagatt ctcctggagc ctgcccggca gaagattctc ctccgcatcg
    10621 aaagcagtcg ggatcggggg gaagattccg ttcagttgaa gcacgtccgc gtcctccggc
    10681 atcacttgtt cttcggacca aatgataacc tcccggggac agcgcggcaa ctgctccttc
    10741 gcgccgccct cggacaagcc cgctggccgg cccggaggcc ggaggcggcc gtggggatca
    10801 aaacagccgg ggaaatcgca ggcgcggggg aaggtcggtt tcgtggtagg agagttcgca
    10861 gccgtagcca tagggatcgt agcggtcgga ttcgaagtcg gtgaccgccg ccagccgcca
    10921 ggtgcccttg atccgtttgt acagcaggaa cgtgtagcgc acccgctccc ggtgcgctcc
    10981 gaacgatgtg ccggggccgg cataagtcag acggatatag tcgcccgact ccttgatttc
    11041 gtagagctgc agggtgtcga gattgggcca ccgggcaaac tggcgctcga tatacgaccg
    11101 aagcgacagg tagccgtgct tgcgaatcaa cttctccagc gtatccgcct gggccgggtc
    11161 gaggagcgcg gagaaccgct ccggctggcc ctgaaggacg gcttgctgca actcgtcgaa
    11221 cagcccgcgc agcgtgtcga tcacgggcgg aagctccgag tggggagccg cggtgcggtt
    11281 ttcggcgaca ctgcacgcca aagcgctgcc caacaagagg atcacccatg ccgagaatat
    11341 ccggaggctc ttcataggca aaatcctccc tctgtacgta actatcggcg ccgcggatgg
    11401 ggaaacttga acaaaatcga agcccgccac cgggggcggg acacgcggga gatgaaaccc
    11461 tcgagcaggc cgcaggggcg gccggccggg ctcagccgac cttcttctcc gccttggtgc
    11521 ggagccgcgg cggctttcct tcggtgatcg tctcctccga gatgatcact tccagaacgt
    11581 cggactggga cgggagcgag tacatggtgt cgagcatggc ccgttcgaaa atggagcgca
    11641 gcgcccgggc cccggtcttg cgctgctggg caatcctgac cgcggcgcgg agcgccccgg
    11701 tttcgaacac gacgttgacg ccctccattt ccaggagctt ggcgtactgc ttggtcagcg
    11761 cgttcttcgg ctcggtgagg atattcatga gcgccgcctc atcgagcgca tgcaaggcgg
    11821 cggtcaccgg cagacggccg accatctccg gaatcagccc gtactccatg aggtcggccg
    11881 ggatcacgtg ctcgaacaga tccggggcgt cttcccggat atgcgtcgac tcggcatcaa
    11941 aacccacctg cttcttgccc agccggcggg cgatgatccg ctccagaccg tcgaacgccc
    12001 cgccgcagat gaacaggatg ttcgtggtgt tgatctgcac gaacgcctgc tcggggtgtt
    12061 tgcgcccccc cttcggcgga atgttggcca ccgtcccctc cagaatcttc aagagcccct
    12121 gctgcacacc ctcccccgac acgtcgcggg tgatcgacgg attgccgtcc ttgcgcgcaa
    12181 tcttgtcgat ctcgtcgata tacacgatcc cccgctccgt cttctgctgg ttgaaattgg
    12241 ccgcctggta gagccggacg aggatgttct ccacatcctc cccgacatag cccgcttcgg
    12301 tgagcacggt ggcgtcggcg atcgtgaagg gaaccttgag aaacttcgcc agcgtccgcg
    12361 cgatgagcgt cttgccggtc ccggtcgggc cgagcagcag aatgttggac ttctccagct
    12421 cgacattgtc gctgtccttc gcgaggctgc tgttcaggcg gtgggagagc tcccagttga
    12481 tccgcttgta gtggttgtag accgcgaccg cgaccgtccg cttggccttc tcctggccga
    12541 tcacgtagcc gtcgagatag ctcttgatct ccgcgggcgc gggcacgtcg cggatctctt
    12601 ccggaagagc aacatccggg gccgattgga gaaggtcgtt gcagagctcg acgcactcgt
    12661 tgcagatgta cgactcgtag cccgaaaaga gccgtttcgc ctggctggcc ggcttgccgc
    12721 aaaatgagca gcgctgcggc ggacggggtt tctcggagtc cgctgacata tcctatatcc
    12781 gtttcccgac cggcgattcc ctcgcccggt ctagctccta cttgcgctcc gaccgcttga
    12841 aatcgtagac cttgtcgatc aggccgtagt ccttggcctc cgaggccgac atgaagaagt
    12901 tccggtcggt atctttctcg atccgttcga gcggctgccc ggtatgctgg gccaaaagat
    12961 cgttgaggcg ctctttggtc cgggtgaact ctttggtcat gatcacgatg tccgagatct
    13021 gcccctgggt gccggcgagc ggctggtgga tcatcacccg gctgttgggc agcgccgccc
    13081 gcttgccgtg ggtgccggcg gccagcagga acgcccccat cgaggcgcaa atccccatgc
    13141 acgtcgtcac cacgtcgggc ttgatgaact gcatcgtatc gtagatggcc agccccgccg
    13201 tcaccgaccc gcccggcgag ttcagataga caaagatgtc cttgtccgga tcctccgcct
    13261 cgaggaagag catctgggcg atgaccaggt tggccacgtt gtcgtcgatc ggcgtcccga
    13321 tgaagatgat gcggtccttc agcaaacgcg agaagatatc gtacgcccgc tccccgcggc
    13381 cggtctgctc gaccaccatc ggcaccaggt attgcgcctg tatcttttcg tccataatct
    13441 cctgcataac tccttggacc tcagtccgtt ttcgtcgtcg cggctattcc ttcttcacaa
    13501 cctcggcctt ggaaatgagg aaatcaagca ctttttcctc gcgcaaggtg tccgtgatgt
    13561 tgtcgtactt gcccgaccgc cggagcccct cgcgcgcctc cgcttcggtg atctcatagt
    13621 tctccgcgaa ccgctttatt actttgtcgg tatcttcccg cgtaacctgt atgttttcct
    13681 gccgggcaat atggtcagag agcaggttcc actgcagcgc cgccgtcgcc atcccgtggt
    13741 acgcgccgcg cagttccgct tcgtccgccg tgctgtcctg gcgccggagg tccttgacca
    13801 tcgcgtcgag gtactccttc accagtccgg cggggaccga gaccttgttc cgctcaatca
    13861 cctgctggac gatttcgttc cggtggtacc ggcgcaactc ccgctccttc tgggccttca
    13921 gatcctcccg gatcttcatc cgcaactcca gagccgtctg ggcgtgtccc gtcctcttgg
    13981 ccaggtagtc gtcgaacgcc ggctgaatct cctcgcgcac ctctttcacc gttgtccggt
    14041 agcgcacggt cgctcccgca aactcctcat ccgggtaatc cgcggcgtag tgcacttcaa
    14101 tttccctggt gtcgccggcc gtgagccctg gaagttgctc gcgaaattcc ttgaccgtca
    14161 acggattaga gagctctatg accgaatcgg ggtaggcctt ctccggctcc tccttggggg
    14221 aatcgaacgt ctgcacgagg tcaacgacca caatgtcccc ctcaccgacc gcgcgggtga
    14281 ccgtacggcg ttcggcgtac gccttccgaa cctgctccgc ggcccgatca acctcctcat
    14341 cggtcaccgt cagatcgtag aacgagactt tcagattgtc gaacgcgatc ggcggaatag
    14401 tcgggaagac ctccacgcgc gccgtgtaat tgaacccgcc gttgtcggtg tagttcaggt
    14461 cggtgaccgt ggggggcgta gcgaccggga gagcgcgctc gcggagcgcc tccgatatcg
    14521 tcgccttaat cagttcatcc gccacgtcgg ctttgacctc gtcggcaaac tgcgatttga
    14581 tgatgttcaa cggggcgtgc cccttccgaa aacccttgag cgtcacgtcc cgctggacct
    14641 cgctgaactt ggactccagc tttttgttca cctcggccgg ctcgatctcg atcgacacct
    14701 cgcgcaccag ttcttcgagt tccctgattt cgaccttcac gttcgtctga cctttccgcg
    14761 gttgcccggc tgtcgtggcc ggcggcggcc gtcgcggttg cgctttcggt ccgccggcga
    14821 gaacatccgt gccggttaag gatgtccgtt cctgtaatgt ccggggcgtt cctcccgttc
    14881 cggcaaatcg gctgagggga cgccgcggct ggtgcgaaag gggggattcg aacccccact
    14941 ccggatggaa ctggatccta agtccagtgc gtctaccaat tccgccactt tcgcctgcaa
    15001 taacttacgt tggctccggc cgaagcgcaa ccggctttct ggacaattcc cgcgccgccg
    15061 gaagctcgcg ccgggagttc gccagttaag ccgccgtgtc ctcgaaccgc cgcctacgcc
    15121 gcccgcgcgt cggcccccgg ccgtcggccc accgggcttc tcccgcgccc tcgccgcatg
    15181 aactccaccc cgagaaacac agccgtcaac gccaggtgga gggcgaaata cagccggttg
    15241 ccatcctgcc agcgccggta ttcgaagctc ggaaagagcc catgggccgc caacagcgag
    15301 ccggccagga gcagtccgag cgcaatcgcc gtgcggaggg ggcctggagc cgccactgcc
    15361 cgcagttctc ccaaccggcg cccgttttcc tcaatatata cgagcacgag cggaatgaac
    15421 agcaacgccg gcggcagcag caggcgactc tcccttatat ggccaaagag aagacccgac
    15481 accaggtacg cgccggccgt caacagcgcc ccgaatcgca ccgcgtcgta ccaagcgggg
    15541 ctctcctccg gccggcggaa cgcctgagtc agccccaccg gccacaggaa acccagcgac
    15601 acgatcagcg agaacaggga gtcgcgcgtc cgcgcccagc tttcgaaatt gaaggccagc
    15661 gaccacacgg gcgagccgct gaccgcgccg agcacagccg cccggaacac gaccagcacc
    15721 gcgaccggcg ccagcgtcgc cagaagcggg cgggcaatcc cgactttcgg gttggtgcgg
    15781 aacaggagga acagcaccgg cagaaaggcc gcgcttgtct cgcgggcaat catggctccc
    15841 gcaatgcagg ccgccgccgg ccagtaccgg cgacccaggg caaaggccat cgccgacggg
    15901 agcaggatgt agcaccagaa atcatcccat gtccacaccg gctcgaagtg ggccaggaac
    15961 aatggaagcg acagcagcag aatcaccata cccgcccgac tccgtgccgg gctgaactgc
    16021 agcgaccgaa ggaaatggta gaacgccgga ccggtgacga agaagagcgc gaactgcaga
    16081 aggaaaaacg cgctccgcac cgacagcccc gtctgctccg ccaggaaacc gaccaccgcc
    16141 gtggtcaccg gccgcacccg gaacacgtca aaatcccggt ggtaggcgag caaatcgtcc
    16201 caggcctcgg cggagagatg gaacgaactg ggactgatca ggtggttcag cagactcagt
    16261 ccggtcagca gcccggcaat caaccagacc gcgcgcgttt ccgatgaacc tgacacccgg
    16321 atagacctcg ttcccctgtg atttcggccc gactcgccgc cgcaccgcca catgcctgca
    16381 gccgtacggg cgagtgatga tagagagcgc cgggcgcgcg ccgcaagcaa aaacggcgca
    16441 atcggcccgg cgccgccgcc ccccccggcc tatccgcccg cccgatctcc ttgccccgcg
    16501 ccgccccgtc gattatcttc cctctgaaat ggcggttcaa ccggccgggc aatccccctt
    16561 cgtagtcagg ccgaccggtc cccccgcctc aggcggagcg ctgacgtgca cgagcttggc
    16621 attgcccagt caattgtcga caaggtgctg gcgaaaatga cggccgaggg ctacccggag
    16681 atcgtcgcca tcggactccg gatcggcgcc ctgaccgacg tggtccccga tgccctcacg
    16741 ttcggattcg aatcgtcggt cgtcggcacc ccgctggccc gaacccgcct ggccatcgaa
    16801 accgtcccgg tcgccggagt ctgcgagacc tgcggccgga cctgcgccgt cgaagagttc
    16861 ctgttcgtct gcccgcactg cggcagccgc gacatacgga tgacgcaggg agacgaactg
    16921 gaaatcgcgt atattgaaat accggagccg tccccggaga tcccgggcga caagaaacca
    16981 ggcgggcggg aaagcggccg ccccgcaccg caggagtgac gatgacggaa cgcgttgagg
    17041 tcaaagagaa ggtgctctcc gaaaacgacc ggctggccgc cgacctgcgg cggcagctgc
    17101 gggaccggcg ggtgttcgcc ctgaacctcg tgagttcgcc gggatcgggc aaaaccagcc
    17161 tcctggaacg tacgctggcc gagttcaacg ataccatccg gattgcggtg gtcgccggcg
    17221 atgtgcagac cgagaacgac gccaaccgcc tgaaagcggc cggcgggcgt atcgtccgcc
    17281 ctatcgtcac cggcgggctg tgccatctcg atgcccgcat ggtcacgaaa gcggtcgccg
    17341 aaatcgacct cgccgccctc gacctgcttg tcgtcgaaaa cgtcggcaac ctggtctgcc
    17401 cttcgagcta tgacctcggc gaggacatga aagttgtcgt catcagcacc accgaagggg
    17461 acgataagcc gctgaagtac cccggcatgt tccggcggtc atcggtgatg gtcgtgaaca
    17521 agatcgatct cctcggcagc tccgatttca ccctggcgaa agtcaaggag aacgccctct
    17581 cgatcaacgg caacctgaag atattcgagg tctcctgccg gaccggcgag gggttggaca
    17641 agtggtacca gtggctgcgg gatcaggtgg cggccagcaa ggcgtctccc tgaaccaagc
    17701 ggactcgtcc gcgggccact catgctgtcg gcgcaagaca tacagagaca acggctgact
    17761 gtgaacggag tcgtgcaggg agtcggattc cgccccttca tttaccgcct ggcccaccgg
    17821 ctggcgctcg ccggttcgat ccgcaacgat gcgcgcggcg tccacatcga ggtcgagggc
    17881 cccgcggcga agctcgacca gttccgccgg ctcctgcgcg ccgaggcccc ggcgctggcc
    17941 cgcatcgatt ccctccgcgt cgagccgctg cccccgcgcg ggcagaacgg cttctcgatc
    18001 gagccgagtc ggcacgggga gacggcagcc gcgctcatcc ctccggatat cgcggtgtgc
    18061 gccgactgcc tgcgcgagat gcatgatccc tccgaccggc gctaccgcta cccgttcacc
    18121 aactgcacca actgcggtcc gcgcttcacc attgtcgaag gcatccccta cgaccggccg
    18181 aaaacgtcga tgaagcgttt ccccatgtgc cctgcctgcg agcgggaata ccacgatccg
    18241 ctcgaccggc ggtttcacgc gcagccggtc gcctgcccgg cctgcggccc gcgactgatc
    18301 gcgcacgacg gcgcccgcga gctggcggtc gccgacccgg tcgcctgggc cgtcgatctg
    18361 ctcgcctcgg gccgcatcct cgccctgcgc ggcctcggcg ggttccacct ggcggtcgat
    18421 gcggccagcg aagaggcggt ccggcgcctc cgcgaccgca agcagcggca gcagaaaccg
    18481 ttcgccctga tggcgcccga tgtcgcgcgc gtgcggaaat tctgcgaggt ctcgccggaa
    18541 gaagagcagc tcctgaccga cgtgagccgc ccgatcgtgc tcctgcggcg gcgccccggc
    18601 gaaaccgcca tcgccccctc ggtcgtcgac gacaacatgt actacgggtt catgctcccc
    18661 tacacacctc tccaccacct cctcctgcac gatactttcg atgccctcgt catgacgagc
    18721 ggcaaccggg ccgaggaacc gatagtgacc gagaacgccg aggcgctcgc gcggctgggg
    18781 aatatcgccg acggattcct gctgcacgac cgcgacatcc tccagcgctg cgacgattcc
    18841 attgtccggc gcctggccgg cggcacccgc atcgtgcgcc gcgcccgcgg gtacgtcccc
    18901 ctgtcggtcc ctctcccggt gcggctggag cggccgatcc tcgcggtcgg cggagaattg
    18961 aagagcacgg tggcgctggg ccgcgccgac caggtcgtgc tcagccagca catcggcgac
    19021 ctcgataacc cggccgcgct cgcgtttttc gagcacgccc tctcccacct gcaaaaactg
    19081 ctcgagtgcg aacccgaact gattgcctgc gatctccacc cggagtacct gtcgaccaag
    19141 tgggcacaca cccagcaggc gctcccggtg atcggggtcc agcaccacca cgcccacctc
    19201 gtgtcggtcc tggcagataa cggctgcacc gagccggcaa tcgggctgat cctcgacggc
    19261 agcggttacg ggaccgacgg caccatctgg ggtggcgagg tcctcatcgg cgacgccgcc
    19321 gcattccggc gctttgcctg gctcgaaccg gtgccgctgc cgggcggcac ggtcgccatc
    19381 gagcagccct ggcgcatggc gctgagctat ctcagctatg cctacggggg cgaggcccgg
    19441 cgtttcccgc tcccgctgtt cgaccgcctc gacagcctgc agatcgacat catcctcgcg
    19501 atgatcgaca agggcctgaa cagccccctg acctcgagtt gcgggcggtt atttgacggc
    19561 gtcgccgccc tggcgggcct ggcgctcgag aacacctacg aggcgcaggc ggcgatcgcg
    19621 ctggagatgg ccgccacccg ggcgggcggg gcgctgcccg atgtcgaacc ggccgcgccg
    19681 ccggccggcg ggggtccgct ctccttcgcc cacctgatcc gcgaggtcgt ccgccgcgtc
    19741 cgctccggcg atccggtcga gcagatcgcg gcgcagtttc acgccgcgct cgcggagctg
    19801 ttcgccggtg ccgccctcgc cgcccgggcc gcgaccggcc tgacgaccgt cgctctcagc
    19861 ggcggtgtct gccagaacgc gctctttttc acccgcctcg cgcaccggct ggaaaacgcc
    19921 ggcttccgcg tgctcgccca ccgcgaggta cccaccaacg acggcggcct ggcgctcgga
    19981 caggccgtga tcgccgacgc cgtccaccgt cgcacgagct gaggagtcgc catgtgtctc
    20041 gccattcccg gaaaagtgat tgagattttc gatgaagacg gcctgacgat gggccggatc
    20101 gactacgccg ggacggtgcg caccgcctgt ctcgattcgg tgcccgaggt gcgggtgggg
    20161 cagtacgtgc tcgtccatgc cggcttcgcc ctcaacgtgc tcgacgaaga cgaggcgcgc
    20221 cggacgctcg atctcttcga tgagatcgcc cggcagaacg ccgaggaggg gctcgatccg
    20281 ttcggggagc ccctgccgcc ggaggcgccg tgaccggcct gaagtatctc gatgaatttc
    20341 gggatccccg gcgggccgcg gccctgctcg gcgagatccg gcgcaccgtc acccgaccct
    20401 gggtgatcat ggagatctgc ggcggccaga cgcactcgat catccgcaac ggcctcgacc
    20461 agcttttgcc gccgacgatc cggctcgtcc acggtccggg gtgcccggtg tgcgtgacct
    20521 cgctcgagct catcgaccgc gctctggcca tcgccgcccg ccccgatgtg atcttcgcct
    20581 ccttcggtga catgttgcgc gtgccgggat cgtcgaaaga cctcctgacc gtgaaatccg
    20641 agggtggcga tgttcgcatg gtgtattcgc cgctggacgc cctccgtctc gcccgcgcgc
    20701 accgcgaccg acgggtggtg tttttcgccg tgggtttcga gaccaccgcc ccggcgaccg
    20761 ccaccgccat ccgccaggcg cgccgcgagg ggctggacaa cttcctgttg ctggtcgccc
    20821 acgtcctcgt gccgccggcg atgcgggcgc tcctcgaggc gcccggcaac cgcgtgcagg
    20881 cgtacctggc cgccggccac gtctgcgccg tcaccggctg ggaggagtac tgcccgatcg
    20941 cggagggcta ccgcgtgccg gtcgtggtca ccggcttcga gccggcggac attctcgacg
    21001 gcatcctggc cgccgtgcgc cagctcgaga ccgggcaggc ccgggtcgag aaccgctacg
    21061 cgcgggctgt ccgggagcag ggcaacccgc acgcgcgcgc cgccgtcgag gctgtgtacg
    21121 aagtgtgcga ccggaagtgg cgcgggatcg gaaccatccc gcagagcggc taccgcctcc
    21181 gccccgaatt cgccgcccat gacgccgagc gcatattccg gctggccgat ctcgaggtgc
    21241 tcgagccccc cgaatgcatc agcggcctcg ttctccaggg cctgcggcgg ccggtcgatt
    21301 gcccggcgtt cgcccggcgc tgcacaccgc agacgccgct cggcgccacc atggtgtcct
    21361 ccgagggcgc ctgcgccgcc tactaccgct accaccgccg cgcggcggaa agctgaaggg
    21421 ttacccgcta tgcccgatga acctctcccc gagccgtcct tctcctgccc gctgcccctc
    21481 tcggcccccg cgaccatcca gttggcccac ggcagcggcg gccggctcgc caacgacctg
    21541 atcgcgcggc tctttgtcga ggctttcgac aatccgatgc tgcggcggca ggatgaccag
    21601 gccacggtgg aaatcgacgg cctgcgcctc tccttctcga ccgattcctt cgtcgttgac
    21661 ccgctcttct ttcccggcgg cgacatcggc gacctggccg tgaacggcac cgtcaacgac
    21721 ctctccatga acggcgcgcg cccgctcttt ttgagcgccg ggttcatcat cgaggagggg
    21781 ctgccgatcg cggacctggc ccgcgtcgtg cagtccatgc ggaaggcggc ggccgccgcc
    21841 ggcgtgcaga tcgtcaccgg cgacaccaag gtcgtcaacc ggggcaaagg ggacaagctg
    21901 ttcatcaaca cggccgggat cggagtcatt tcccacccct acgacatcgg cgtcgaccgg
    21961 ctccggccgg gggatcgcgt gttgatcagc gggccgctcg gcaaccacgg cctggcggtc
    22021 ctctcccggc gcgagggtct cgctttcgaa agccccattg tcagcgacac ggcccccctc
    22081 aacgggctcg tggccgcgct ggtcgccggg gttggcggcg cgctccacgc catgcgcgat
    22141 ccgacccgcg gcgggctggc cgccaccctc aacgaattcg cccgcgcctc ccgggtcggc
    22201 atccgcctgg tcggggcgca ggtgccgatc gacccgcccg tcgccggcgc ctgcgagctg
    22261 ctcggcctcg accccctcta cgtcgccaac gagggcaagc tgacggcggt ggtgagcgcc
    22321 gaggcggccg atcgcgccct cgccctcatg cgcgcccatc cgctcggcgc cagggcggcg
    22381 gtcatcggcg aggtccacgc cgaccggccc ggagaggtga ccctgcggac cgtgctcggc
    22441 acggagcgga tcgtcgacat gcccgtgggc gagcaattgc cgcgcatttg ctgacgtctc
    22501 tttccggaat tccgtgcccc gttttcggat gcgcgaacgg cccgcccccg ttaccttggg
    22561 acaaaaagct taaccggaga tacgaaccac cgaccatgag ccacctgccg cctcccgcca
    22621 tcatgtaccg ggcgcttcgc gaccgtgacg ccaccttcga gggcgtcttc tacgtcggcg
    22681 tccgcaccac cggcatcttc tgccggccca cctgcccggc ccgcaaaccc aaagaggaga
    22741 acgtccagtt cttcgccttc ccgcgggagg cactccaggc cgggtatcgc ccctgcgccc
    22801 ggtgcgctcc gctcgaggtc gagcggcccg ccccggcgct ggtccgcgcc ctgcgcaacc
    22861 tggtcgatga gtcccccggc gggaagattt ccggcgccct actccgcgcg cgcgggatcg
    22921 atccttcgac cgcccggcgg cagttccagc gctactacgg catgaccttc cacgcctacc
    22981 agcgcgcccg gcgcctgggc ctggcgctcc acgaaatccg ccggggggaa tcggtcatcg
    23041 gggcccagat cgaccacggg tacgagtccg ccagcggctt cttcgaggcc ttccggaacg
    23101 ccttcggcgc accgccgagc cgcctcgaga cggtggcttg cctgttcgcg caggcgatcg
    23161 agacgccgct cggtccgatg gtcgcggtgg ccgacgactc cgggctgcat ctgctcgatt
    23221 ttgtcgaccg cggcgggtgg cagcaaaccg tcgggaagct ccgggaacgg tcggcgaccg
    23281 cggtcgtccc cggtccccat ccgcacctcg ccgcggtggc ggagcaactg aagagttatt
    23341 tcgaggggcg ttcgctggac ttctcggtcc cgctcgttcc ggacggcact cccttcgaac
    23401 tggcggtctg gtcgcttctg cagactatcc cgccgggggc cgcctggtca tatgcgcaga
    23461 tggccgcgcg gctcggccgt ccggccgccg tgcgcgcagt cgggcgggcc aacagccgca
    23521 atcgcctggc cctcgtgatc ccctgccatc gggtgattcg tgcggacgga accctgtgcg
    23581 ggtacggcgg cgggatatgg cgcaagcagt ggctgctggc ccatgagcgg cgggtgctgt
    23641 cgcgccggga aacgagtccg gttactccgg gcccgacagg atgaagtcgc gaaccttcag
    23701 gccgtagcgc tccgggtcga tcagccggac gatgcggcgg ttgatctgat cggcgcgggc
    23761 cagatcgacc cactccggtt ccggcaggag gccctcccgg ttgcccacga tctggacata
    23821 gggccggtcg tacagctccg cattagcggc catgatcacc gcgatctcat ccgtgctcaa
    23881 cagcgccatg ctcccggccg ggtagatgcc catcacctcg ttgaacaggg cgatcaggat
    23941 cggattgaat ttggccgtca tctggaaccg catcttccgg atcacttgat ccggcgccat
    24001 cgctttccgg aaatacaccc ggtcggaggt gagggcatcg aacgtgtcgg cgatcgtgat
    24061 aatctgcgag tacaggttca gcggccggat gtcgtggagc agggggtagc cggtgtaatc
    24121 ggcgttgatg tggtgttcga acgccccgcg cgccgcccgc gccgtgtgct ggtcgagctt
    24181 catgctgcgc aggatcgtct tggcccccag cgccgggtgc atctgcatct ggatccagtc
    24241 gttctcatcg aacgagtccg gcttgttgat caggtcgcgg tccagccgca ccttccccgc
    24301 gtcgtggaag agcgcggcaa agccgagctg tgacaggcgc ggacggtcga ggcccaggcg
    24361 gatccccaga gtcagcgagt acaccgacac gttgaccgag tgggcgtagg tgtagtcgtc
    24421 gaaatccttg atggccgaca gctccaccag cgaatccttg tccttcgaca ggtggtcgat
    24481 gagcgcgtgc acgaccctcc gggtcttggc cagattgaca tcgcgctcca cggccgtgcg
    24541 ggcgagcagt tccgagactg cggagacggc gcggaaaaac gtcgtccgcg cctggagccg
    24601 gagctggcgg cgccgctccg cttcgtcttc gtcgtcttcg ttttcgtccg gcagcgcgag
    24661 gaggaagacg gtggcgggga tgtggcggtc gagttcctcc tgcagttccg cgagactccg
    24721 cccgcggggc gtcagcgcgg ccatgaaact gaaaaacccg cgcagatgat cctcggtgac
    24781 ctccggcagg aaacggaccc cgccgattcc gagggcctcc cagtcggcgg tgacggcggc
    24841 ggccgcgccg ctggggtcgg agaagcggac gaagcgctcg ttggtgaaat agcggccccg
    24901 caccgccttg atcgccacct cggcatcggc cttcagcccc ggctggagca gctcgagaaa
    24961 atgggcgcac tgcttcacga acgccttgtt ggccggctcc atgattcggg cgttccggta
    25021 cagcacgaag aaggcgttga cgagcttttc gtcccgttcc gtttccgcca cccgggtttt
    25081 gccggtatcg actgcggtcg tcatgcctca cctccggtcg gcgctttgcg gcggctcaga
    25141 cagcgggtcg cgcgccggcg gatatccggg cgccacgagc cggtcaggcg ttgcaggagc
    25201 gcgcccgctt tctcgctcgg gttctgctcg agggcatcga atgcggccgc tcggaaaaac
    25261 gtccggatcg gattccgcgt cgggttgaag cgggcgatca gccgggccag gtgcggcacc
    25321 gcccgctctt tcccccggcg cgagtacgcc accagcagcg cgatctggtc ctggggatcg
    25381 agttggccga acgattcggc cgtgaccgct ccggcgagcg cctcgaacgc gcccgccccg
    25441 gaactcccca tcagcgcggc catcgcctcc cggtgaactt cttcgtccgg gtcgaatacc
    25501 agccggccga gaatctcgag ggctccggcg tgctctttcc cccgcaccgc ctccaccagc
    25561 tcgatccgca cccgccggtc ctcgtgggcg accgcctgtt tcaggtgctg gagcgcccgg
    25621 tcgtcgccga tgtggccgag gatcatcacc gaattccgca ccacgtacca ccgtttgtcg
    25681 tacacaccct tggcgaggat ttcctccttg ccgcggccgt gatccaccag gtaccggcag
    25741 agcgtctcgc ggtgggggcg gtgctccagt tcgccgagta gatcggtcag cgcgctcagg
    25801 gcctgccagt cgaacaggtc gaggtaccgc cggaggtcgc tctcggtgat ctcggtgtgc
    25861 tcgttcagcg ctttggccag gaggcgcagg cgctcccggc tcccggcggt catgtagatc
    25921 ccttccagcc ggtcggccca gcgcggcttg gctccccgga tgcgctctcc gagattcctc
    25981 agggaccgca gcaggaaggc cgcatgcgcc acctggccct gggccacgaa ctcgttcaac
    26041 attttctcgc agatgatcgc cgtctcgtcg aatcccttga gcgtggtctc ctgcatgagc
    26101 agctcgatga ggaggtgcga cgttgactcg tacaggttga aggcggcatc ctgcgccagc
    26161 agttgctcca tccgggcctg ctcgtcggcg ctgaggctgt tctcgcggga gaggatgtcg
    26221 cgcacgctgg gcaccggcac cgggggcaga ttggacagcc ccatggcttc cgccgccgcc
    26281 tgcgcccgga ggcgggcggc ctcctccggc tcaacctcga acacgccgcc gggtgtcagg
    26341 tgcccgtcca ccgtctccgg cgccgctctc ccctcgagcg cgtcttcgct caggaatatc
    26401 gcttcatacc cgtcgggcgt atcgccccct gcccggcgcc gctcgtcctc ccggtggaag
    26461 tactcctgga cgcggaaacc ggcgtcatac tcggccaggg ccacatcctc cgccgactgg
    26521 aacgagaaac cctcgggcgc cgcttcccac agaagcgacg ggagatcgcc gtggcccgcg
    26581 ggcgccgtga gataagtccg gatcgctccg agcagacggt cgatgctcgc cgccgtcagc
    26641 ccccgcttga aactgagttc cgtgatgccg gcgctgaaaa agatggcggc gagattgtcc
    26701 tcccgcgact tgtcgcggtg cacgatttcc ccgttgcgca gaatgacccc gttgtcgacc
    26761 cccagccgga tctccccctg ctcctcgagc aggtcgagca gccggctggc gaatgactgc
    26821 cggagcgact gcgggagcga attcgtctcc gggtacatgc agacagcttt gatcgtccgc
    26881 aggagatccc gcaggagaac ggcgatttcc tgatccggag ggcggtcggt attcggtgtt
    26941 tccatcatcc tgccgttggt tttctgatca cagtatcggc agaatgcccc gcccgattga
    27001 ccggccgccg ggccggtcaa cgaaaaaccc ggcggcgccg ggttttccgt caaagcctcc
    27061 ggtcggtcaa gcgcttacag cacttcgatc ttcttccgcc cgttccgcac tttccgggtc
    27121 gcgttgcggg tgtcgaatac cgccttggcg ttgtccacta tccactggta gtcgtactgg
    27181 gcgtggttgg tcacgatcac caccaggtcg gtcttgcgca gcagcgcggc cgtcagccgg
    27241 gtggaccgcc gggccgtccc ctcccactga atcgacggga cgtgcgggtc attgaacacc
    27301 acccgcgcgc cgcggtcctc cagcagcttg atgacgtcca gcgccggcga ctcccgcaca
    27361 tccttgatgt tcgccttgta ggcgatcccc agcacgagca ccgaggactt gttgagcgcc
    27421 ttggcgaacc gctcgttgag catcctcatc acccggtcga ccacgtagtg gggcatcgag
    27481 gagttgatgt cccccgccag ctcgatgaac cgggcatagt agttcagcga cttcagcttc
    27541 cacgccaggt agtggggatc gatcgggatg cagtgcccgc cgagccccgg accgggatag
    27601 aacggcatga acccgaaggg cttggtcgcc gccgcgtcga tgatctccca cacgttcagc
    27661 ccgagccggt cgcacatcag cgccacctcg ttcaccaggc cgatattcac cgagcggaaa
    27721 gtgttctcca gcagcttgac catctccgcc gcctgggtcg acgacacggc gtagatgtcc
    27781 gggatcgact gctcgtagaa tgtcttggcc acctgggtgc acttgggcgt gatccccccg
    27841 accacccgcg gcgtgttttt cgtcgtgaac cgctggttcc ccggatccac ccgctcgggc
    27901 gagaaggcga ggaagaaatc ctcccccgct ttcatcccct tctcctgaag cagcggcagg
    27961 attaggtcct ccgtcgtgcc cggataagtg gtcgattcga gcacgacgag cgtccctttg
    28021 cgcatgaact gcttgaccga gccgaccgcg gccaggataa acgacacgtc gggatctttc
    28081 gtcttcgaca gcggggtcgg cacgcagatc gaaatggtgt ccatctcgcc gagagcgcgg
    28141 aagtcggtgg tggcgcggaa ctttttggcc gccaccatgt cggccaccac ggcatcgggc
    28201 acgtcgtcga tgtccgaccg tccgctgttg agcagcctga ctttcggctc ggcaatgtca
    28261 aaaccggtga cctcaaaccc ggcgtgcacc agctccaccg ccaggggcag tccgacgtag
    28321 cccagaccga tcacaccggc cttggctttc ttgctcgtga tcttctccat aagctgccgg
    28381 tgaccggaca cgccggactg cttgggagct gatttcttgg ccatacagga ttactccatc
    28441 accttattca ttctcggcgg acagacgttc tttccagtac gcgaacgtct cccgcagcgt
    28501 ttgcttcaga ctgtatcggt tggcaaatcc gagttcttca acggcccggc ggttggatcc
    28561 gcgcaggacg gggatgtcgg cggcgcgcca ctttccccgg tccaccgcga tggcgacggg
    28621 tttcgccgtg aacccgagca ggaggcggag gacggtctcg atcgaggtcg cccgtccgga
    28681 gcacaactgg tagacttcgc ccggggcgcc gcgttcggcc aacaggcggt aaccgcggac
    28741 gatgtcgcgg acatcgctga aatcccgccg ggccgcgaga ttccccacgc gcacccgcgg
    28801 gggctgcagc cccaggtcaa tggccgccgc ctgacgggcg aaatcggcga tcacgaaaat
    28861 cggcgcctgc cgcggaccgc tgtggttgaa cgaccgcgcc accgtgaccg gaaggccgta
    28921 gcggcggaaa tgcgagcggc agagatgctc cccggccgcc ttcgaaatgc cgtacggcga
    28981 agtggg
//
LOCUS       DAOPOE010000035        27623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2220_length_27623_cov_5.326502, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000035 DAOPOE010000000
VERSION     DAOPOE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27623
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_27623_cov_5.326502"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKM94_06400"
     CDS             complement(<1..212)
                     /locus_tag="PKM94_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOD66309.1"
                     /translation="MTEEYKKPSAEEILIEDYRPKYREHFRALNYEWLERYFSVEPYD
                     RIVLADPQREVIGRGGCVLFALIGEDV"
     gene            complement(231..713)
                     /locus_tag="PKM94_06405"
     CDS             complement(231..713)
                     /locus_tag="PKM94_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOD66310.1"
                     /translation="MTGRKRRRHDLIEVRESRIQGRGVFALRRIRKGQRIIEYAGERI
                     TPEEVDRRYDDLAMDRHHTFLFEVDENTIIDGAVGGSAASYINHSCDPNCEAVDVDGR
                     IFIYAARNIQPGVELTYDYQYDLDEALTEELKRRYPCHCRARRCRGTILVDKRRRRAG
                     "
     gene            complement(1116..1370)
                     /locus_tag="PKM94_06410"
     CDS             complement(1116..1370)
                     /locus_tag="PKM94_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOD66311.1"
                     /translation="MVEEKTFTEEFKVTGQQLVETVKKLIHEGNVRHVIIKNEEGKSL
                     IEIPVTVATVGALLLPVLAALGAIAALVADCTIVVVKTEK"
     gene            complement(1734..2078)
                     /locus_tag="PKM94_06415"
     CDS             complement(1734..2078)
                     /locus_tag="PKM94_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOD66312.1"
                     /translation="MSRLAGLKNTLMYNGPFTLFGPAEQTFAALPAGTVEALLQDPAT
                     LSNILLYHVTSGVFKAADVVTLTEAAMANGGTVEIAVVDGNVKVNSATVLFVDVAARN
                     GVLHIIDGVLIP"
     gene            complement(2111..2722)
                     /locus_tag="PKM94_06420"
     CDS             complement(2111..2722)
                     /locus_tag="PKM94_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOD66313.1"
                     /translation="MFTRKFTLMTALVAALAIGMTGCSNDDENPMNSGSPNPTMDIVE
                     TAAAAGNFTTLLAAAEAADLVDALKGAGPITLFAPTDDAFAKLPAGTVEALLADKDAL
                     TAILTYHVVPGAVTADQVVRLSSAETLNGASVTITLGGDGTVRIDNAVVTATDIKATN
                     GVIHVIDAVLIPGASSSARMNPLGAKASLSVPAGSPRRSGAAS"
     gene            complement(2800..3006)
                     /locus_tag="PKM94_06425"
     CDS             complement(2800..3006)
                     /locus_tag="PKM94_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOD66314.1"
                     /translation="MKSFDVIAVALMVVGGLNWGLVGLLHFDLVATLLGDATLLSRLV
                     YMVVGLGALYQVVQWKAIQRRWVA"
     gene            3790..4188
                     /locus_tag="PKM94_06430"
     CDS             3790..4188
                     /locus_tag="PKM94_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD66315.1"
                     /translation="MDNRIIVSTILIAVFAMAVPGLADSTSQKAEKTYKIHGFLGESS
                     TVAAPNKTVTLLDADSNTALANTNSNFFGKYAFKKLPPGHYLVKVAEKVMEVYLIDKD
                     IRLDVDLSANDGTMNYAAGAAAQAAKPAEK"
     gene            4282..4563
                     /locus_tag="PKM94_06435"
     CDS             4282..4563
                     /locus_tag="PKM94_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66316.1"
                     /translation="MGGGGGSESQIAFCPDGSYFESYESGYYGSGQWGQAGQSRASGT
                     WSITGTIETGTITITYANGKQETVRYETTGESGCYKFGGRLYCYNGACD"
     gene            4929..5498
                     /locus_tag="PKM94_06440"
     CDS             4929..5498
                     /locus_tag="PKM94_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66317.1"
                     /translation="MKRSHLSDQQLWESVLVGKARAWDELVSRYGPLVYAVCRQVGIS
                     ESDAADVFQHSWIALYENRFRLEDPTRIASWLLTTAKREALRYRQRQARLQSDDCLAE
                     RADERPLPDEELRRLELQARLELALRSIDRLCRDLLRAFFFAGEDQSYEQIAAAFHIA
                     PNSLGAKRRRCLDKVRAVLIRLGYLEERK"
     gene            complement(5728..6051)
                     /locus_tag="PKM94_06445"
     CDS             complement(5728..6051)
                     /locus_tag="PKM94_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66318.1"
                     /translation="MHDKAGGRARRPPLAYPEVIRIDHFCVGLYDKSVGGADALRDAR
                     PPGLFLFNPQLYRDPSRFEVQAAVVFDRRSAHADRRRRRPGIEYQTRGHLDQSGTRRR
                     TFPGD"
     gene            6087..8849
                     /locus_tag="PKM94_06450"
     CDS             6087..8849
                     /locus_tag="PKM94_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD66319.1"
                     /translation="MEANETDIAARRFLRTGSTGVHGESAVAAACEKLIQKAFRTSIR
                     RALGLAERFHARAQAHGGRVLGQTAWRSLGRMHHFSGRHAEARHCYRAARTLCGDDLL
                     IRGRIDRTLIDINMYLGNDRAAAAAGRAALRAFSALEAESDLAQTRVNLGNLYHRRDK
                     PVEADRLYRQAALFFARSGNKLALARTNYNRANVLVQLFQMDEAEKLYRDALAAYTES
                     GYELDACDTRYALAWLNLLTGRFHQALSDLAACEATYRTAGDPRGVALCLLDRAEVFL
                     QLGLYRDALDLAGRAGKRFRTLKLEYESGKAHLFAAYALTGLGRKDRARAAWARAEQG
                     FAALRHAGYLGTLHLLRTDLDQGSPGTRQTALRAARRCFDRAALFFWQAVCDLKAIEL
                     SGRTPVYLARLRRNPAAWCVPHLYAAWQVAEGDLHLVSGRRAAALTAWRKAADRLDLV
                     RSALPPMELRNQYARAAKSPHERLVESYFSDTPREAAAWSERARTAGIWNAGARHPAL
                     AAARRRVQQSLGALSARVQTLAQELMIPAHRSIGAGAALPSAARLNFLRREIRECLLT
                     IDQAPTAEALDTSRLGARIAAASRQAPIVQFQVAGGDILAFVHVGGEVATRRFVGGRE
                     RLERLAERWRFCIERNVVRHAPAQESRTPLEQALWREFGAWLWAALEIPDEAAQVIIV
                     PDGPLANLPWQAIECDGGLLGERHALVLAPSLRHHWFARTRPGAGLPIHLFRGAGSDL
                     ASAQGELARLAALAPGRTIMHAPATRASWPQEGEFFIWHYAGHAQLVVENPFYSYLRL
                     EDGPLFAADFQIRSCRVELATLAACRSGEEVTLPGEESTGFVRALLEMGARNVLAGRW
                     PVLDQSTALYMEHFYAELLSSKSLVQAMHNAERRTRLKYPSAYHWAAFGLHGAGFLGE
                     DHAKTP"
     gene            8833..10239
                     /locus_tag="PKM94_06455"
     CDS             8833..10239
                     /locus_tag="PKM94_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66320.1"
                     /translation="MRRLLKILSLIGIVLLTGPRPAMIRADAERPNHTPGDVVCRVLP
                     GSTIEEINQTYGTAVKGHLPVTDVYLLMIPQGQNPDTIAARIDADARVDFCRPNYYLA
                     APEGFQRSSPFVDAELIGTFEDQPAAVTLDLATAALVSTGAGARIALIDGGVAANHPV
                     FASFPGDLVSRWDYLEEDSVAQADTAGSCAAHGTFVAAVLQLVAPGADVYVYKVLDTA
                     GQGNAYAIAAAILQAIDDGCDVINLSLGMTRVDESLDDALRTARQSGVSVVASAGNDS
                     TNLASLFPFPASRSNVIAVAALDSVNVKADFSNYGVRIDVCCPGTRVYSAYADTLFAW
                     WDGTSFAAPMAAGVIACLRSRHPGLTPERIDTILARSSHNVDSLNPGLEAQLGAGLID
                     PPAALALAGQITRGDVTLDGRVDINDLTALVNYLFRGATLDVLADADVSCDSAVNIAD
                     LTLLVKYLFLGAAMPCGA"
     gene            10437..11639
                     /locus_tag="PKM94_06460"
     CDS             10437..11639
                     /locus_tag="PKM94_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66321.1"
                     /translation="MNGRTKWLSLMGAGLLLTAMIGCSESPTAPQTGGSDGTAVESKA
                     PAADSLQLRARVQTVDQNRLRLTLEGETDTVATLQNCQIVRYSGFSEAPVPFTEIRVN
                     DSVEVAGHRYQNACVNAYRIGICEEPGAVRYAARVETVNRDRRMLTFAGKPDSVIANL
                     NCAVVRLNGNSEAPVPFDEINPGDSVQLYGERNQNRYVYARHLGVCSPGGGGYDVAVR
                     DTITSIDYAAGTFTIAGRTELITVDSETVIWTNLRILHEQPDENGRDRMGMALSDGGN
                     FYRSDHDSILAFTDVAVGDVIELRADIVNETTLRAAVIKLTPECLSRCLAFSDRLATI
                     DVAARLVTFENETRICWVCPGAKLRDRDGAEITLADFAVGDFVAVKGYPLAGDTLKIS
                     QMALTAEP"
     gene            12417..13298
                     /locus_tag="PKM94_06465"
     CDS             12417..13298
                     /locus_tag="PKM94_06465"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD66322.1"
                     /translation="MKRLLLVLSLLGMMTAFFGCSNDPQTTGPAVGQDTPGTLVQRPE
                     FIDNRPDAVKAAFTKLGTPIESSDRVVADGFSAADPNPTPAHKYAYVIGISDYDGTAN
                     DLNFCDDDARDVIAFLNSQGFTVRSDIDYSASAANIQAGLIWLRDAAVPGDEVAFFYS
                     GHGTKVRPSGGSSIISRDLYYLTHAYVMGYLNAANCTKKTINLDACVIGDFHADCKTG
                     TVLSTASVSKYSYDAPDLQNGAWTYFFLDGAINQGLAYNEAISTYAEVNMKAWATANR
                     LKVDPKHTDMYTGDLDI"
     gene            complement(14054..14143)
                     /locus_tag="PKM94_06470"
     tRNA            complement(14054..14143)
                     /locus_tag="PKM94_06470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14107..14109),aa:Ser,seq:tga)
     gene            complement(14309..15517)
                     /locus_tag="PKM94_06475"
     CDS             complement(14309..15517)
                     /locus_tag="PKM94_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD66323.1"
                     /translation="MRKRLVFSVLVLFAALPAAAERLTVERGSDLQSVINYAASGDTI
                     VIAAKTFSARPRLFTDSLCGNCLDPHTPVEASHGFIIRGKSLELIGESRTESRLETNA
                     GYGLFIVDCPEVRIANLTITGGRRDKDGNATDAGIVVRNSRVDISGVTIAGNTHRLDS
                     VVVGIGGVFGREGSEIEIRDCRIVDNGWDGVALYRGAAATVTDCVIRDGRGAGIGVTW
                     DATCAAYRNDISGYWKGIGAFGTSWVIARNNLVHDNLGWGIIATGASYMDIANNVVHH
                     NGNCGVAPWSSEARGRIANNIITANGWREQWVCPCVGVWNYGDWAKWEFRFNIVYGNQ
                     DGNYEAIWDQSGINGNLNGDPLFAGEADFRLTKGSPGINGGDTLIYNADGSRSHIGLE
                     GGPQAKRVRR"
     gene            15639..16745
                     /locus_tag="PKM94_06480"
     CDS             15639..16745
                     /locus_tag="PKM94_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66324.1"
                     /translation="MAHYAALLLSRQALRPCAAAPWVRQTMAALDWLRAEGLTVISSV
                     GMPTWELICAAAGLRGVPQVVIVPGPGGARGTAPDSLCAAFGLGETASFHPLPVDSRK
                     AFPSARDQVVFSLADLLVPISIRPGGFMATRLAEYRSAGRAVVDRFAVAYRPREEPLR
                     YAISERELNPDLEKLSRHYVTHWTRAARGPWPDELPIDYYRSILASPAYPRTALDTLR
                     RILTSRRLVASDRHLPRGVRAVAFSGLSPHRVLPLIRWRARYREMSFEPYGVAIDASV
                     AREHGILPVEYCDPATRPALPPDDHWRLQSSGVVGDWVREEEYRCEGDFDLGTVPEEA
                     LLAFCRFPAEAGDIARTSGVRCLPLLARDPRPPG"
     gene            complement(16754..19621)
                     /locus_tag="PKM94_06485"
     CDS             complement(16754..19621)
                     /locus_tag="PKM94_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66325.1"
                     /translation="MSVAGMRMLSIALSLWILTPPMLSAGRLAPELEERLQGKAAGEK
                     IRVWIGVTGAVDPRALKAALTSSYPDGATRHREGMRRLKEAHGARQANVLGFLRGLEA
                     RGEAARVRPHWLGNIIEAELTPGQIRAAARRGDVDLVAAPPELTLIAPEPAPSSATAV
                     GANLSVIKANLAWQAGYTGAGRLICSFDTGVDGAHPAIASGWKGLDGNAAAAWFYPLD
                     EEPFPHTVGNDGHGTHVMGLLVGHDDATGDTVGVALDARWISAAVIDIHGAPIFDAFE
                     WAVDPDGDPTTFGDVPDVVNHSWGIRFAECRELFYTVVENMEALGIVNIFAAGNEGSL
                     PESVRNPADRALDSLDCFAVGNIIHTMSPPVIYSSSSRGPSPCTGGIKPNVCAPGYAV
                     RSSLPGNTYGFLTGTSMAAPHVSGLVALLRQKNPNATPDQIKAAILTTAQDYTYSLPD
                     NTYGWGVIDCQAALNALPGPTAAPNLRIWSFPHPLVHAGDVVTGPVILQNRGTVVANV
                     AGTITGSHPALSVLDGSVSFGTAPAWDTVRSSDVLAVEVSDTVTEGSILSIDFQIAGS
                     GYSVPAKLYFVVEPRSQRLQADHDVNNLHCTITNYGTYGLDTGSFFPANGIGFRTAPL
                     FDNEVYESAFLLGRSITQVSDGARNSAGEPDGDFRVAHGGNIVRSTPGKKADQETSAR
                     FDDSRAENPIGLAVVQETYAFTEASFDDFIIFRYILTNSGGVEITGLRAGLYLDWDVE
                     QFSLNAGGWDAADSVLWMAYNDAGTLSLFRGIKLLDGPLASAFTADASLTTYYGGGLS
                     DGWTEAEKYAALAAGFGSAETFKTATKDLMQVLAAGPITMAAGQTDTVAFAVVTGNTL
                     GEIRANADAASTVYEDVIADCCLGLRGNVNGDPDENITVVDVTYLIAYVFRGGPPPLC
                     PEEANANSDAGGAITVADVTYLVAYVFRGGPAPAACGTTAP"
     gene            19939..20463
                     /locus_tag="PKM94_06490"
     CDS             19939..20463
                     /locus_tag="PKM94_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOD66326.1"
                     /translation="MNLGHLSPDTLRAMLADFAKNWLAHDGLWFQAVERRYGLETAIA
                     LDAEAWEQFTAIEARRIMALHRLPENGGLEALKTALGFRLYAFINEQEILQEERAADG
                     SLVSFEFQMVDCRVQSARRRKGLPLFPCKSVGLVEYEGFARTIDPRIRTQCLCCPPDA
                     AAGADQYCAWRFSL"
     gene            complement(20562..22283)
                     /locus_tag="PKM94_06495"
     CDS             complement(20562..22283)
                     /locus_tag="PKM94_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD66327.1"
                     /translation="MVVSGIGKRALLFCGAVLAAGVPAAAAEVAGRVYDQASGEAIPG
                     AVVRVGNSLVQLTVADTTDAEGEYLVSGLASGTDYTVMASAAGYQSAALTMAAVPLTH
                     DFALRSFADGYLVGTINQGTVHYAYAGGACYRSGGEVEAEEGFFPHIRVPSASVDSLV
                     GLIGVDPAATPTSDSLVWEACRRVWHWLAVNARAAGSHPQWTEANNFLMGYDDGWPSV
                     EAMAETYFQFGFLPWGTCMSRAHILASLLFTAGLPDRDLGIAETRWMFRYSQHMFAAV
                     RVNLRWLYLDATRMVGELPDYAALASCPVDGSEDIDYCYPYSLKVIPGTAAAGVPLLT
                     HRTSNSRHLCVAAPPDGAVTLCPAIAVWVLQSDQTAPGVKVNGQMCEALPDGLYCELA
                     AAEGALPIVAEGMAAGVPVRDSIRVERAACPLDADGDGWCYGCDNCAAVANPDQKDAD
                     GDGIGDACDNCQDHANADQVDADKDGRGDACDNCPMAENPDQADADGDGIGDACCCTA
                     RGNVDMAGGITVADVTHLVGYLFRGGPGAGCPAHADVNGNGGTEVADLTHLVAYLFRA
                     GPPPAGC"
     gene            complement(22469..23395)
                     /locus_tag="PKM94_06500"
     CDS             complement(22469..23395)
                     /locus_tag="PKM94_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD66328.1"
                     /translation="MRFLVTGGAGFIGSNLAAALAADGNQVRILDNFSSGRQENLADL
                     PASVEVVDGDIRDYWTVVQAVAGVDYVLHQAALPSVPRSVKNPLTSNEVNVGGTLNVL
                     EAAKNARVKKFVMASSSSVYGESEELPKHEGMRPSPLSPYAITKLTCEYYCKVYWELY
                     GFPTVALRYFNIFGPKQDPASQYAAVVPRFITALMRNESPTVFGDGEQSRDFTYIDNC
                     VQANILAATNDRIKGDYFNVACGGQFTLNELLDRLRRIIGTSTQAAYVDPRPGDIRHS
                     FAAIDKLGAFGYKPSVSFDEGLRRTVAFFKAK"
     gene            complement(23685..25229)
                     /locus_tag="PKM94_06505"
     CDS             complement(23685..25229)
                     /locus_tag="PKM94_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOD66329.1"
                     /translation="MRAPSAPPLPWRRRLMYAAVTTVVLLGLIEGGARLLTSQLDSHA
                     MPAGGIGWQAVFFRSLFTWNEPDPDLLWRFRPNLHNPLIRTNSRGLIADEVPYARPAG
                     TLRVMVLGDSSPVGLGLKDRTFAFPEVLTRLLAAALDPDVRVEMINASVTGYTSEQLA
                     RLYERELLRYEPDYVVVYCGNNDASVSGSWSDLDLLRSQRAAGLRRLLARSAAYRLLR
                     DLLVRPVRDRGALGAPLVPRVSPERYAANLERIIRACRDDRTRVVLVAPPQRYLWPAG
                     LQFKIFSHVTGEDGEFVFPPQLARLLDRPILYCLDGRRFSVTDTAVDHMTRAVYRSAA
                     SDPLDPAAAAAHWQEAAGLAPGDPVAANNLGVALWRNRAWSEADRAFAAARRRWAGQD
                     AASAVERDAAGSPILFNRAINLLDSLADTSMVLALGSVPFVLLDSALQADWMSLRIKQ
                     PYLEALWALREKPGVTVVTVAEEFRANGGDGLFVDHCHPTRRGHEIIARALADVILRQ
                     ERGGGE"
     gene            complement(25401..26369)
                     /locus_tag="PKM94_06510"
     CDS             complement(25401..26369)
                     /locus_tag="PKM94_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66330.1"
                     /translation="MAGDGKFRALRFVRWAAGPAAAAALWLGACGDDAQERAARLFPA
                     GPPIAHLEEFPFNADSLLPGGGAGPEEPPTVRKIGALAVDSSSDPGYLAYDFIGEAAA
                     RYTADEAEVRVTIAQFAEPIAAYGWYAAHRPNGAATDTIGRQSFWTGTRLYVYKGSYA
                     IVAEAGDTSAAARAAAERLAREAANRATGEMRLSPYHILFPMHDRVAASDRYVGYGFL
                     GAPRLNDVYTMDFVAGIDTARWFLTTDTSGLKLVVLRDWAEDGTRRLTVPAVLPFEPE
                     SAVYVEHPRYGRIIAGMAAQKLVGVVGYRPERQEQKLRSWLEGLRK"
     gene            complement(26330..27202)
                     /locus_tag="PKM94_06515"
     CDS             complement(26330..27202)
                     /locus_tag="PKM94_06515"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOD66331.1"
                     /translation="MIHDILRLVRPAQWLKNGIMVLAIVFAGELAHADKIILMACAIA
                     LYCLLSSAVYTFNDVVDAERDRLHPLKKNRPLAAGRIRVATGAVLAGVLAAAGLVGAW
                     FINGPFFLSAVTFLALNLLYSLWWKHVVILDVLTIALGFVVRAYAGAFAIDVPASTWF
                     LINTLLLALFLGLGKRRHELLYLDKEAQAHRKTLVMYSPYLLDQLIAVVTAAMLVIYM
                     LYTFSTEVMHKLGTDKLFVTIPFVVYGVFRYLYLIHRKDQGGSPTRVLIDDRPILLTV
                     MLWLVTASFVLYVL"
     gene            27327..>27623
                     /locus_tag="PKM94_06520"
     CDS             27327..>27623
                     /locus_tag="PKM94_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOD66332.1"
                     /translation="MRKRQLKGAGGHYLIAVNQQAGQFSDRLLRRLTGAIRSQGSHYT
                     VIEPASAADLGATAREYCGLAKAARPAPPPVAKRGRVTALVAAGGDGTVNALAAV"
BASE COUNT         4690 a         9472 c         8682 g         4779 t
ORIGIN      
        1 acgtcctcgc cgatcagggc gaacagcacg cacccccccc gcccgattac ctcccgctgg
       61 ggatcggcga gcacgatacg gtcgtacggc tcgaccgaga aatagcgctc gagccattcg
      121 tagttcaatg cgcgaaaatg ctcgcggtat ttcggccggt agtcttcaat caggatctcc
      181 tcggccgaag gtttcttgta ttcctccgtc atcgtcccgc cccggttcgg ttatcccgcc
      241 cgccgccgtc gcttgtccac gaggatagtc ccgcgacacc gtcgggcccg gcaatggcag
      301 ggataccgcc gtttgagttc ttcagtaagc gcctcatcca gatcgtactg gtagtcatag
      361 gtcagctcca ccccgggctg gatattgcgg gccgcataaa tgaatatcct tccgtccacg
      421 tcgaccgcct cgcaattggg atcgcacgag tggttgatat aactcgccgc gcttcccccg
      481 acggcgccgt cgattatcgt attttcgtcg acttcgaaca ggaaggtatg gtggcggtcc
      541 atcgccaggt cgtcgtagcg gcggtcgacc tcctcggggg ttattcgttc cccggcgtac
      601 tcgataatgc gctgaccctt ccggatccgg cgcagggcga acactccgcg cccctggatg
      661 cgggactcac ggacttcgat caggtcgtgg cgccgtcgtt tgcgaccggt cataacggta
      721 tgcacgtttg atggttttcc gcccgaactt ctcacccttt aacgccgccc caataaatgt
      781 ggttccaccg ttcccgtcca ccggaatctg tcgagcagcg gtcgcccggg ccccggccgg
      841 cgaacgtcca tctcgggctg gatactccgt cagccgcctg cgctgtcgcg cgcaggggcg
      901 tggttacgag gtcgatcgag gcgccgccgg cccggctgag tttcccccgg actgcggctg
      961 gaaactccgg ccgcggcggc tcccctgccg cccgcccaac ctctgatttc tccccgtcct
     1021 gggacggccc ggtcggcgcc ggagtgcagg gacgcccctc tccggcgggg tttgaatcat
     1081 cgcgccttgg cagacgaggg agacctacgc gaggttcact tttcggtctt gaccaccaca
     1141 attgtgcagt cggcaaccag tgcggcgatc gcccccaggg cggccagcac cgggagcagg
     1201 agcgccccca cggtcgcgac cgtgaccgga atctcaatca gcgacttgcc ttcctcgttc
     1261 ttgataatca cgtggcggac gttcccctcg tgaatcagct tcttgaccgt ctccaccagt
     1321 tgttgaccgg tcaccttgaa ttcttccgtg aacgtcttct cttcgaccat ggggcacctc
     1381 tcaggattga cgtgatccgt ggcgggcggc ggtccccgcg gcgtgcgctg cgggatggcc
     1441 cggatacttc caggcggcaa ggtagagcgc gctccgtccg gtggcaacgg gttttctcga
     1501 tgcggacgcc gtctcaccgc gccgcggagg ttccctgctc cccggcccgg cgccgaacgc
     1561 cccgcccgcc gaccttcacc cgtatgccgc gcggccggcc gcatcgggcc tgccgttcga
     1621 caaagctccc tggcgtccgg ggagcacccg gcgatccggg aaaatggagc gccgaagatc
     1681 gtttccgatc tccggcgcgc cgtacctgtg cccggttgtc ggttcgcagt ccgctacgga
     1741 atcagaaccc cgtcgatgat gtggaggacg ccgttcctcg cggcgacatc cacaaacagc
     1801 acggtggcgc tgttgacctt cacgttgccg tccacgaccg cgatctcgac cgtcccgcca
     1861 ttggccatcg cggcctctgt cagcgtgacg acgtcggcgg ccttgaacac tccggaagtc
     1921 acgtggtaca ggaggatgtt gctcaacgtc gccggatcct gaagcagagc ctcaaccgtt
     1981 cccgccggaa gagccgcaaa agtctgttcc gccggtccaa acagggtgaa cggaccgttg
     2041 tacatcaggg tgttcttgag accggccagc cgggacaccg tgtagagcga cagatacttg
     2101 gtgaaccggt tcagcttgcc gctccggacc gcctgggcga tccagccgga accgagagcg
     2161 acgccttggc cccaagcggg ttcatccgcg ccgaggagga ggcgccggga atgaggacgg
     2221 catcgatcac atggatcacg ccgttggtgg ccttgatgtc ggtcgccgtg accaccgcgt
     2281 tgtcgatcct gaccgtgccg tcgccgccga gagtaatagt gacgctggcg ccgttgagag
     2341 tctcggcgct ggacagcctc acgacctgat ccgcggtgac cgcacccggc accacgtggt
     2401 aggtcaggat agccgtcaaa gcatccttgt cggccagcaa tgcttcgacc gtaccggccg
     2461 ggagcttggc aaaagcatcg tctgtggggg caaacagcgt gatcggtccc gcacccttga
     2521 gcgcatcgac gagatcagcg gcttcagccg cggccagaag agtcgtgaaa ttgccggccg
     2581 ccgcggccgt ctcgacgatg tccatggtgg gattcggaga accgctgttc atcggattct
     2641 cgtcgtcgtt gctgcagcca gtcatgccga tcgccagggc agccacaagg gccgtcatca
     2701 gtgtaaactt ccgggtgaac atctttttgc tccttactat actatgaacg ttttcttttg
     2761 ttttcggtat tcgtccgcat ctgtcggtgt tgcggccggc taggccaccc accggcgctg
     2821 aatggccttc cactgaacca cttgatagag agcccccaaa ccgaccacca tatacaccag
     2881 ccggctcaag agggtggcgt cgccgagcag tgtggcgacc aggtcgaaat gcaggaggcc
     2941 caccagaccc cagttgagtc ccccgactac catcagggcg acagcgatca cgtcgaatga
     3001 tttcattcca gtactccttt ttgcgtattc tgttcattat ttgtctaagt tctgacttat
     3061 gtaactccga agctggcgga tcggttcacg aaaaaggaga aaatggcgaa aaatatcata
     3121 accaattgta atcacactag ttatgttata ttctcatggg ctatgtcagg ttcacgatga
     3181 tcgccaaagt gtccatatta ttgaacagat acgagacggg tgaatcactc gacagcactg
     3241 ccggtggcct gcggggagat cgccaaaggc agcagggccg aaaaacgcct gcgcggaggg
     3301 ccaccgccgc gtgcgcgatt cgattgcccc gccgttaggc acgccgtccg ttacagccat
     3361 caccaggtat ctgagtagtg tcctcgtgtc agataagaca agagcggggc gcggagtcga
     3421 tccgagagta ctgcgacagc tcatcatgag ttgtcttact cagaatgtca gggccggcga
     3481 tcggagagtc acccggtccc cgccgacgcc cggcgtcggc gacggaatgt ctatgcccga
     3541 ttagggagtc gggggcttgt cgcgccccgg ttgcaggccc tgccgcgtgg cccggagtct
     3601 tgtcgatgtg agggacaccg caattcttgg cgagactatc gtgcgagcta tcatcaggga
     3661 gtgagcatcg agctccgcgg gaggttcgac cgacttcaaa gtatcgtttg aaacgaggtc
     3721 cgggatatga gtatcttccg gtatgcatga acctcaccgc agtaccacca ctgtaaagga
     3781 ggagtatcat tggacaatcg aataattgta tcaacgattc ttatcgccgt cttcgccatg
     3841 gctgttcccg gcctggccga ctcgacctcc cagaaggcgg aaaagactta taagattcac
     3901 gggtttctgg gggagtcatc gacggtggcg gcgcccaaca agactgtcac gctgctcgat
     3961 gccgacagca acacggcgct ggctaatacc aacagcaact tcttcggaaa gtacgccttc
     4021 aagaaactgc cgccagggca ctatctcgtc aaagtagccg agaaagtcat ggaggtctat
     4081 ctgatcgaca aagacattcg tctcgatgtc gacttgagcg ccaatgacgg gaccatgaac
     4141 tatgccgcag gggccgcggc gcaggccgcg aagccggccg aaaagtaagg cgaggggact
     4201 ccccccggcc ggccggaggg ggacaccgat ctcgccaaac aattctccgg caagtactac
     4261 agctacaccg gaggctcgac attgggcggc gggggcggtt cggaaagtca gatcgctttc
     4321 tgtcccgacg gcagctattt cgaaagttat gagtcgggct actatggttc cgggcaatgg
     4381 gggcaggcgg gccaaagccg cgcgagcggc acctggtcaa tcaccggcac gatcgaaacc
     4441 gggacgatca ccatcactta cgccaacgga aaacaagaga cggtacgcta cgagacgacc
     4501 ggtgagagcg gctgctacaa attcggcggc cggttgtact gctacaacgg cgcctgtgat
     4561 taggaggaga gcatctatta ttgtggatat tccggcgtga ggttccagat ccgattgcgc
     4621 cactcctcgg ttggcggaag cgtgctcgga gagggtcggt tgccagagtg ccacgggagg
     4681 caggtgagcc tgttcctgac acacggtgtg atcagcgtct cgaccatccg gaccggttca
     4741 tgaccggtga cgccggttat gtcggcaacg tctgctcatc gaccgggtga ggttacccgc
     4801 catcacaagg aacagcggag ctgagtgcgg cgtgcagaag ttccgccggg cgacagccgg
     4861 cggatttcgt ttgccttatc cggcatttga gttactttga cggcagaact gcgcccccag
     4921 ggagaaccat gaagcggtcg cacctgtccg atcagcagct gtgggaaagc gtgctcgtgg
     4981 gcaaagcccg ggcctgggac gagcttgtca gccggtacgg gccgctcgtc tacgccgtgt
     5041 gccgccaggt gggcatcagc gagtccgatg ccgccgatgt attccaacac tcctggattg
     5101 ctctctacga aaaccgcttc cgtctcgagg acccgacccg catcgcctcg tggctgctga
     5161 caaccgccaa acgggaggcc ctgcggtacc ggcagagaca ggcgcggctg cagtccgatg
     5221 actgcctggc cgaacgggcc gacgagcgac cgctgccgga cgaagagctc cggcgcctcg
     5281 agctccaggc ccggctggag ctcgccctgc ggtcgatcga ccggctgtgc cgcgatctct
     5341 tgcgggcgtt cttcttcgcc ggggaggatc agtcgtacga gcaaatcgcc gccgccttcc
     5401 acattgcccc gaactcgctg ggcgccaagc ggcgccgttg tctggataag gtgcgcgccg
     5461 tgctcatccg tctgggatat ctggaggaac ggaaatgacc cagaccgcct ctgttctagc
     5521 ggagaaggag tgatcatgag cccagagaag cacctgactc ggaagcggct aatcgaaatt
     5581 gcgcgcgcgg gcactccccc gtcggattct cacttggccg cctgcgacgt gtgccgggag
     5641 gcgtacgaga tgctgtcccg gtactactcc ctgatcggcc gccctcccct tcccgcacca
     5701 ccggccgact tcctgaggcg ggccaggtca atcgcccggg aaggtgcgcc gtcgggtgcc
     5761 cgactggtcg aggtggccgc gcgtctggta ttcgattcct gggcggcgcc ggcggcggtc
     5821 ggcgtgcgcg gaccggcggt cgaaaacgac cgccgcctgc acttcgaagc ggctggggtc
     5881 gcggtagagc tgcgggttga acagaaagag accgggtggt cgtgcatcgc gcagtgcgtc
     5941 ggcacctccg accgatttgt catagagacc gacgcagaag tggtctatcc ggatgacctc
     6001 ggggtatgcc agtggcgggc gccgcgcccg cccgcccgcc ttgtcgtgca caccgccgca
     6061 acccgcgtca ttgtctccag actccaatgg aagcgaacga gacggacata gcggcgcggc
     6121 ggttcctgcg cacgggatcg accggggttc acggcgagag cgccgtggcc gccgcctgtg
     6181 agaagctgat ccagaaggcg tttcgcacgt cgattcgccg cgcgcttgga ctggccgagc
     6241 ggtttcacgc ccgggcgcag gcgcacggcg gacgggtact ggggcagacg gcctggcgct
     6301 cgctcggacg gatgcaccac ttctccggcc ggcacgccga ggcgcggcac tgctaccggg
     6361 ccgcgcgcac gctctgcggc gacgacttgt tgatccgcgg ccgcatcgac cgcacgctca
     6421 tcgacatcaa catgtacctg ggcaatgacc gggcggccgc cgccgccggc cgcgctgccc
     6481 tccgcgcctt cagcgccctc gaggccgagt ccgatctggc gcagacgcgc gtcaacctgg
     6541 gcaatctcta ccaccgccgg gacaaaccgg tcgaggccga ccggctctac cggcaggccg
     6601 ccttgttttt cgcgcgaagc ggcaacaaac tggccctggc ccgcaccaac tacaaccggg
     6661 ccaacgtact cgtgcagctc ttccaaatgg acgaggccga gaagctctac cgggacgccc
     6721 tcgccgcgta caccgagtcg gggtacgaac tcgatgcctg cgatacccgc tacgctctgg
     6781 cctggctgaa tcttctgacc ggccgtttcc accaggccct gtccgacctc gctgcatgcg
     6841 aggcaacgta tcggaccgcg ggcgatcccc gcggcgtggc gctctgcctg cttgaccggg
     6901 cggaagtgtt tctccagttg gggctctatc gagacgccct cgacctggcc ggccgggcgg
     6961 ggaaacggtt tcggacgctg aagctcgaat atgagtcggg gaaggcgcac ctgttcgcgg
     7021 cctatgcgct gaccggcctc ggccgaaagg accgggcccg ggcggcgtgg gccagagccg
     7081 agcaggggtt tgccgccctt cggcatgccg ggtacctggg aaccctgcac ctgctgcgga
     7141 cggatctcga ccagggatcg ccggggacgc ggcagaccgc gctgcgcgcg gcgcgccgat
     7201 gcttcgaccg ggcggcgctg ttcttctggc aggcggtgtg cgatctgaag gcgatcgaac
     7261 tgagcggacg cacgccggtc tacctggcgc gccttcgccg caatccggcc gcgtggtgcg
     7321 tgccccatct ctacgccgcc tggcaggtgg cggaggggga ccttcacctc gtctccgggc
     7381 ggcgggcggc ggcgctcacg gcctggcgga aagcggccga ccgcctcgat ctggtccgat
     7441 cggcgctgcc gccgatggaa ttgcgcaacc aatatgcgcg cgcggcgaag tcgccgcacg
     7501 agcgcctggt agaatcgtat ttcagcgaca ccccgcgcga ggcggcggcc tggtcggaaa
     7561 gggccaggac ggccggaatc tggaacgccg gcgcccgaca ccccgccctg gcggccgccc
     7621 gccggcgggt acagcagagc ctcggggcgc tgtcggcacg ggtgcagacg ctggcgcagg
     7681 aactcatgat ccccgcgcat cggtccatag gtgccggcgc cgctctcccc tcggccgccc
     7741 ggctgaactt cctccgccgc gagattcgcg agtgcctgct gacgatcgac caggctccaa
     7801 cggcggaggc tctcgacacc tcccgattgg gcgcccgcat tgcggccgcg tcgcggcagg
     7861 cgcccatcgt gcagtttcag gtagccggcg gcgacatcct cgcctttgtc cacgtcgggg
     7921 gagaggtcgc cacccgccgc tttgtcggcg ggcgggagcg cctcgagcgc ctggccgagc
     7981 ggtggcgatt ctgcatcgaa cgaaacgtcg ttcgccacgc gcccgcgcag gagagccgaa
     8041 cgccgctgga gcaggcgctt tggcgcgagt tcggcgcctg gctgtgggca gcgctggaga
     8101 tcccggacga ggcggcacag gtcattatcg ttcccgacgg tccgctggcc aacctcccgt
     8161 ggcaggcgat cgagtgcgac ggcgggctgt tgggcgaacg ccacgcgctt gtgctggccc
     8221 ccagcctccg acaccattgg ttcgcccgca cccgtccggg cgccggattg ccgatacacc
     8281 tcttccgcgg cgccggatcc gacctggcgt ccgcccaggg cgaacttgcc cgcctcgcgg
     8341 ctcttgcccc cgggcgcacg atcatgcatg cgcccgcgac ccgggcgtcg tggccgcagg
     8401 aaggcgaatt cttcatctgg cattacgccg ggcacgcgca actggtggtc gaaaatccct
     8461 tctattccta cctgcggctg gaggacggtc cgctctttgc cgccgatttt cagatccgct
     8521 cgtgccgcgt ggagttggcg acgctcgcgg cctgccgctc gggcgaggag gtcacgctgc
     8581 ccggcgaaga atccacgggc ttcgtgcggg cactcctgga gatgggtgcg cgcaacgtcc
     8641 ttgccgggcg gtggcccgtt ctggatcagt cgaccgccct gtacatggag cacttttatg
     8701 ccgaactact gagcagcaaa tcgctggttc aggcgatgca caatgccgag cgccggacgc
     8761 ggcttaaata cccgtccgcc tatcactggg ccgccttcgg cctgcacggc gccggctttc
     8821 tcggagagga ccatgcgaag actccttaag atcctgtccc tgatcgggat cgtgctgttg
     8881 accggcccga ggccggccat gatccgggcc gacgcggagc ggccgaacca cacgcccggc
     8941 gatgttgtct gccgtgtcct ccccggttcg acaatcgagg agatcaacca gacctacggt
     9001 accgccgtca agggacacct cccggtcacc gacgtgtacc tcctgatgat ccctcagggc
     9061 cagaaccccg acacgattgc ggcccggatc gacgctgatg cgcgggtgga cttctgccgc
     9121 cccaactact atctcgcggc gccggagggg tttcagcgga gttcaccgtt cgtcgatgcc
     9181 gagctgatcg gtacttttga agaccagccg gcggctgtga cgctcgatct ggccaccgcc
     9241 gccctggtgt cgacgggggc cggcgcacgc attgcgctga tcgacggcgg cgtcgccgcg
     9301 aaccacccgg tattcgcctc gttccccggc gacctggtca gtcgctggga ttacctggag
     9361 gaggattccg tggcgcaggc cgacacggcc ggttcctgcg ccgctcacgg cacgtttgtg
     9421 gccgccgttc tccaattggt cgcacccggc gccgatgtgt acgtctacaa agttctcgat
     9481 acggccggcc agggcaacgc ctacgccatc gcagccgcca tcctccaggc gatcgacgac
     9541 ggctgcgacg tgatcaacct gagcctgggc atgacccggg tcgatgagag tctcgatgac
     9601 gcactgcgca cggcccggca gtccggcgtg agcgtcgtgg cgtccgccgg caacgattcc
     9661 accaatctcg ccagcctctt cccctttccg gcctcgcgct ccaacgtcat tgccgttgcc
     9721 gcgctggact cggtcaacgt gaaggcggat ttctccaact acggggtgcg catcgacgtg
     9781 tgctgccccg gtacgcgggt ctattcggcc tacgccgaca ccttgttcgc ctggtgggac
     9841 gggacgagct ttgccgcgcc catggcggcg ggcgtgatcg cctgcctgcg cagccggcac
     9901 cccggtctga cacccgagcg gatcgacacg atcctggcgc ggtcatccca caacgtcgat
     9961 tcgctcaacc cgggtctgga agcgcagttg ggagccgggc tgattgaccc gccggcggcg
    10021 ctggcgctgg ccgggcagat cacccgcggc gatgtcacgc tcgacggcag ggtcgacatc
    10081 aacgacctca ccgcgctcgt gaattacctg ttccgcgggg ccacactgga cgtgctcgcc
    10141 gacgccgacg tctcctgcga cagcgccgtc aacatcgccg atctgactct gctcgtcaaa
    10201 tacctcttcc tcggcgccgc catgccctgc ggcgcgtgag gaccggcttc gccttgtcct
    10261 cgttccgacg ccgggacgcc cccgcggccc ccggcgtttc tgcttgattt gccgcaccgc
    10321 aattccgccg cgtcgatccg aactccctgc acgaaacatt ccccgccgcc ctctgcttaa
    10381 caggacacga ggcaagcgag gacaacaaac gtttaatact ctgtcaagga ggtttcatga
    10441 acggacggac gaagtggctc tcgctgatgg gagcgggcct gctgttgacg gcgatgatcg
    10501 gctgcagcga atcgcccacg gcaccgcaga ccggagggag cgacggcacg gcagtcgaat
    10561 cgaaggcgcc cgccgccgac tcgctgcagc tccgcgcccg cgtacaaacc gtcgaccaga
    10621 accgcctcag actcaccctg gaaggggaaa ccgacacggt ggcgaccctg cagaactgcc
    10681 agattgtccg gtacagcggc ttctcggaag ctcccgtccc cttcacggaa atccgggtga
    10741 acgactcggt cgaagtggcc ggtcaccgct accagaacgc ctgcgtgaac gcctaccgga
    10801 tcgggatctg tgaggaaccg ggagcggtgc ggtatgcggc gcgcgtcgag acggtcaacc
    10861 gggaccgccg catgctgact ttcgccggga aaccggattc tgtcatcgcc aacctgaact
    10921 gcgcggtagt ccgcctcaac ggtaacagcg aggcgcccgt cccgttcgac gagatcaatc
    10981 cgggcgattc cgtgcagctc tatggcgagc gcaaccagaa ccgctatgtc tacgcccgcc
    11041 acctgggcgt gtgcagcccc ggcggaggcg gctatgatgt cgccgtgcgg gacacgatca
    11101 cctcaatcga ctacgccgcc ggcaccttca cgatcgccgg ccgcaccgaa ctcatcaccg
    11161 tggactccga gaccgtcata tggaccaacc tccggatcct ccacgaacag ccggatgaaa
    11221 acgggcgcga ccgcatgggt atggcgctgt cggacggcgg caatttctat cgctccgacc
    11281 acgactcaat tctggccttc accgacgtcg cggtcggcga cgtgatagaa ttgcgggccg
    11341 acatcgtcaa cgaaaccacc ctccgtgcgg cggtgatcaa actgacgccc gagtgtctga
    11401 gccgctgcct cgccttcagc gaccgcctcg cgacgatcga tgtcgccgct cggctcgtca
    11461 ccttcgaaaa cgagacgcgg atctgctggg tctgcccggg ggcgaaattg cgggatcgtg
    11521 acggagcgga gatcaccctc gccgatttcg cggtcggcga tttcgttgcc gtcaagggct
    11581 atcccctggc gggcgatacg ttgaagatct cccagatggc gctgacggca gaaccgtaga
    11641 cgtctccacc acaaagtcgg tgagacgccc tcggcgtccg gactccctcc ccgccggacg
    11701 ccgatgcttt cgtcgctatc cgggcaggca tggagagagt agaccggtct tgagctggac
    11761 ggtctcagga tggagggccg gatcagggtc cggccttcct ctttgtcttt ttggcgcgtt
    11821 cccccgcccg ccctccggac ccggctatcc cgtggctgag gggggagcaa gctcacgcac
    11881 gaagtcgccc ggctggtgcg caggtatctt ctcggttcgg gggagggact cgctcacggc
    11941 cactgcttcc ccacgaccgg attgcccggc gcacagccta atccggagcc ctccatcacg
    12001 cagcatggcg tatccatcac gctcccctcg cggccgggcg cagggtcgga gccaagggga
    12061 accgaacgcc ggatgcccgc ctggaatttg actttcacaa gaactcgccg ggcctgcggc
    12121 cggggtcgcc ggaaacctcc ttgccccgtt gtgctcttgc gcctggtgac gccgaaccgg
    12181 gcaggcgaat ttcggccggg ccgacgaaaa aagcgtggac agatttgttg gacagagcat
    12241 atattggggc agcacccaca gctgcggcgt ccgtcctccg gctacgatac ggggggacgg
    12301 gctgtcgaag caaccaagcc gtcgtgtcct tcgggagagg aggtcgtcgc gtttggctct
    12361 ggggtgaact ccctcctcat cagtgttttc aaatcaaccc taaggagagg tctcacatga
    12421 aaaggctatt actggttctg agtctgttgg gcatgatgac ggcgttcttt ggttgctcga
    12481 acgacccgca gacgaccggt cccgctgtcg gccaggacac ccccggtacc ctggttcagc
    12541 gccccgagtt catcgataac cgcccggatg cggtgaaagc tgcattcacc aagctgggca
    12601 cacccattga gtcgtctgac cgggtggtcg ccgacggctt ttccgccgcc gatcccaacc
    12661 cgaccccggc tcacaagtat gcgtatgtca tcgggatctc cgattacgac ggcacggcca
    12721 atgatctgaa tttctgcgat gacgatgcgc gcgatgtcat agcgttcctg aacagccagg
    12781 gtttcaccgt gcgcagcgac attgactaca gcgcctcggc ggccaacatc caggcgggcc
    12841 tgatctggtt gcgcgacgcg gcggttcccg gcgacgaggt ggccttcttc tattccggcc
    12901 acggcaccaa agtgcgcccg tcgggtggtt cgagcattat ctcgcgggac ctgtactacc
    12961 tgacccacgc atacgtcatg ggctacctca acgcggccaa ctgcacgaag aagacgatca
    13021 atctcgatgc ctgtgtcatc ggcgacttcc atgccgactg caaaaccggc accgtgctct
    13081 ccaccgcctc cgtatccaaa tactcctacg acgcccccga tctgcagaat ggcgcctgga
    13141 cctatttctt cctggacggc gccatcaatc aggggttggc gtacaacgaa gcgatctcta
    13201 cgtatgccga agtgaacatg aaagcctggg cgaccgcgaa cagactcaaa gtcgatccca
    13261 aacacaccga catgtacacc ggcgatctgg atatctgacc cctcttcgcc tgttcctgag
    13321 taatgacagc ccgcttcctc ggaggcgggc ttgtcatttg gggtgggcag gggggagcgc
    13381 gcgtgagtgc aagtctctcg tcggaccgct ccgcccaaaa gaaccaccgc ggactgtttt
    13441 tttctgttat ccggacgaac ggatttccag gcaagctccc tctgatagcc aagaggtgaa
    13501 ctcggaacca ggaacctgag cggctgtgtc aacggctggc acgagtatgg cgggttccgg
    13561 gctgtgggtg ctacacagca gaggccgggg accgcgcccg gccttccttt cagcttcatc
    13621 cccccgagcg accgcccgac ggtctgccac gagcggcgtt gccatgacag cctggggggg
    13681 atcagccgcc ggcggtcttc ccgacacgac taacagccag aaacgccact catgaattgt
    13741 ctgcttgacc agcgagtgta cgttgcgtcg cctcgggtgc ggcctgccac cgcatgggcg
    13801 agatggtggc gcgttggaac ttgatcttat ccaccgcctc ggcgaggtgg gcgtagatca
    13861 cggtcgtcgg gatgtccgag tggcccatga gcttcttgac cgtgggcagg tcgaccccgg
    13921 acatgaccag gtggccggcg aaggggtggc ggagggtatg gagccttcga acttcacgct
    13981 caccgactga ggcctctccc ccacttacga gaagcccttc agctaccttg ccgaagagcc
    14041 caaaactgaa atatggcgga ggaggtagga ttcgaaccca cggtgccctc gcgggcacaa
    14101 cggttttcaa gaccgccgct ttcgaccact cagccactcc tccgttccgt tatgtctgtc
    14161 tcgtccactt cggccgccct tcccgccctg cgtccgtctt ccgaaccccc tcgtcgtcca
    14221 agcgtccggt tcagcggatt gcccgccccg gttccgcacc tttcctgcgc tgcacccggc
    14281 gcggccgtgt cccggcgctc ttacgcagtc agcgccgcac tcgttttgcc tgcgggccgc
    14341 cctcaaggcc aatgtgcgaa cggctgccgt cggcgttata gatcagcgta tcgccgccat
    14401 ttatacccgg ggatcccttg gtgagacgga aatccgcctc tcccgcaaac agcgggtcgc
    14461 cgttgagatt cccgttgatg ccggactgat cccagattgc ctcgtagttg ccgtcctggt
    14521 tgccgtagac gatgttgaac cggaactccc acttggccca gtcgccgtag ttccagactc
    14581 ccacgcacgg acacacccat tgctcgcgcc agccgttggc ggtgatgata ttgttggcga
    14641 tccggccgcg ggcctcgctc gaccagggcg ccactccgca gttgccgttg tggtgcacga
    14701 cattgttggc aatatccatg tacgaggcgc cggtcgcgat gattccccac ccgagattgt
    14761 cgtgcacgag gttgttgcgc gcgatcaccc aggaggttcc gaacgccccg attcctttcc
    14821 agtagccgct gatgtcgttc cggtaggccg cacaggtcgc atcccaggtc acgccgatcc
    14881 cggcgccccg gccgtcccgt atcacgcaat cggtcacggt cgccgccgcg ccccggtaga
    14941 gcgccacgcc gtcccagccg ttgtcgacaa tccggcagtc ccggatctcg atctcggagc
    15001 cctcccgtcc gaacacgcct ccgatgccga ccacgactga atccaaccgg tgcgtgttgc
    15061 ccgcgatggt gacgccggag atgtcgacgc gcgagttgcg caccacgatc ccggcgtcgg
    15121 tcgcgttccc gtccttgtcc cgccgtcccc cggtgatcgt gaggttggcg atgcgcacct
    15181 ccgggcagtc gacgatgaac agcccgtagc cggcgttggt ctccagccgg ctctcggtgc
    15241 ggctttctcc gatcagctcg agcgacttgc cgcggataat gaacccgtgg gacgcctcca
    15301 ccggcgtgtg cggatcgagg cagtttccgc agagcgagtc ggtaaacagg cgcgggcggg
    15361 cgctgaaagt cttcgccgcg attacaatag tgtcgccgct cgcggcgtag ttgatcaccg
    15421 actgcaggtc ggagccgcgt tccacggtga ggcgctccgc cgccgccggc agagccgcga
    15481 aaagcacgag cacactgaac accagtctct ttcgcatacg ccgtattaag cgctgcgcgg
    15541 cggcggcggg caagcaaaaa gccgccggga ggccgggcca ccgccgcggc cgcagaatgt
    15601 gattccccgc gccccgcgcg ccgcccagat tcccgctgat ggcccactat gccgctctcc
    15661 tgctgtcccg ccaggcgctt cggccgtgcg ccgccgctcc gtgggtgcgg caaaccatgg
    15721 ccgccctcga ctggctccgc gccgagggac tgactgtgat cagctctgtg gggatgccta
    15781 cctgggagtt gatttgtgcc gccgccggtc tgcgcggtgt gccccaggtc gtgatcgttc
    15841 cgggaccggg tggggctcgc ggcacggcgc ctgacagtct ctgcgccgcc ttcggactcg
    15901 gcgagaccgc ctcctttcac ccgctcccgg tcgactcgcg caaggccttc ccatccgccc
    15961 gggaccaggt cgttttcagc ctggccgacc ttctggtccc catttccatc cgccccggcg
    16021 gtttcatggc gacccggctg gcggagtacc ggtcggcggg ccgcgcggtc gtcgaccgct
    16081 tcgccgtcgc ctaccgccct cgggaagagc cgttgcggta tgcgatctcc gaacgcgagc
    16141 tgaaccctga tctcgagaag ctctctcgtc attatgtcac ccattggacg cgtgcggccc
    16201 gcggaccatg gccggatgaa ctcccgatcg actactatcg gagcatcctg gcctcccccg
    16261 cctacccccg taccgcattg gacacgctga ggaggattct gacatcgcgg cgtctcgttg
    16321 cgtcggaccg ccatttgccc cggggtgtgc gcgccgtcgc tttctccggc ctgtccccgc
    16381 accgagtgct gccgctgata cgctggcgcg cccggtatcg cgagatgtca ttcgaacctt
    16441 acggcgtcgc tatcgacgcc tcggtcgccc gcgagcacgg cattctcccg gttgagtact
    16501 gcgatcccgc aacccggccc gcgctgccgc ccgatgatca ctggcgcctg caatcaagcg
    16561 gcgtcgtcgg cgactgggtg cgggaggagg agtaccgctg cgagggtgat ttcgacctgg
    16621 ggacggttcc ggaggaggcc ctcctggctt tctgccggtt tcccgccgaa gccggcgata
    16681 tagcgcggac ctccggcgtt cgctgcctgc ccctgctcgc ccgcgacccg cgccccccgg
    16741 gttgaggccg gacttagggc gccgttgttc cgcacgccgc cggggcgggc ccgccccgga
    16801 aaacgtaagc gaccaaatag gtcacgtcgg cgaccgtgat cgcgccgccc gcgtcgctgt
    16861 tggcgttggc ctcctcggga cacagcggcg gcggcccccc ccggaacaca taggcgatca
    16921 ggtaggtcac gtcgaccacg gtgatattct cgtccggatc gccgttcacg ttgccgcgca
    16981 ggccgagaca gcagtcggcg atcacgtcct cgtagaccgt gctcgcggca tcggcgttgg
    17041 ccctgatctc cccgagcgtg ttaccggtca ccacggcgaa cgccacggtg tcggtttgcc
    17101 cggcggccat ggtgatcggg ccggccgcca gcacctgcat aagatctttc gtggccgtct
    17161 tgaacgtctc cgccgacccg aaacccgcgg cgagagccgc gtatttctcc gcctccgtcc
    17221 acccatccga cagaccgccg ccgtaatacg tggtcaggga cgcatcggcg gtgaacgccg
    17281 acgccagcgg gccgtccagc agtttgatcc cccggaacag cgacagcgtc ccggcgtcgt
    17341 tgtacgccat ccacagcacg ctgtcggcgg catcccaccc ccccgcgttg aggctgaact
    17401 gctcgacgtc ccagtccagg tagaggccgg cgcgcagccc ggtgatctcg accccgccgg
    17461 agttggtgag gatatagcgg aagatgataa agtcgtcgaa ggaggcctcc gtgaaggcgt
    17521 acgtctcctg gacgaccgcg aggccgatgg ggttctccgc ccggctgtca tcgaagcggg
    17581 cggacgtttc ctgatcggct ttctttccgg gcgtggagcg gacgatgttc ccgccgtggg
    17641 ccacccgaaa atccccgtcc ggctcgccgg cggagttgcg cgctccatcc gagacctgag
    17701 tgatgctccg cccgaggagg aacgccgact catagacctc attgtcgaag agcggggcgg
    17761 tccggaatcc gataccgttg gccgggaaga acgagccggt gtccagcccg taggttccgt
    17821 agttcgtgat cgtgcagtgc aggttgttga catcgtgatc cgcctgcagc cgctgcgagc
    17881 gcggctcgac cacgaagtac agcttggccg ggacggagta cccgctcccg gcgatctgga
    17941 agtcgatcga cagaatgctg ccctccgtca ccgtgtcgct cacttccacg gcgaggacat
    18001 cgctcgaccg caccgtgtcc cacgccgggg cggtcccgaa cgacaccgag ccgtcgagaa
    18061 cgctgagcgc ggggtgggat ccggtgatcg ttcccgccac attggcgacc accgttccgc
    18121 ggttctggag gatcaccggc ccggtgacga cgtccccggc atgcacgagc ggatgcggga
    18181 acgaccagat gcggagattg ggcgcggccg tgggcccggg cagcgcgttg agcgccgcct
    18241 ggcagtcgat caccccccag ccgtaggtgt tgtccggaag agagtacgtg taatcctggg
    18301 cggtcgtcag aatggccgcc ttgatctggt ccggcgtcgc gttcggattc ttctgccgca
    18361 gcagtgcgac caacccgctc acgtggggcg cagccatcga cgtccccgtc aaaaatccgt
    18421 aggtgttgcc cggcaggctc gagcgcacgg cgtaacccgg cgcgcacacg ttcggcttga
    18481 tcccgccggt gcagggggag ggtccgcggg acgaggagct gtaaatgacc ggcgggctca
    18541 tcgtgtggat gatgttgcct actgcaaagc agtcgagcga gtcgagcgcc cggtcagccg
    18601 ggttgcggac cgactccggc aacgatccct cgttacccgc cgcgaaaatg ttgacgatgc
    18661 cgagcgcctc catgttctcc accaccgtgt agaacaactc gcggcactcg gcgaatctga
    18721 tcccccagct gtggttcacc acgtccggga cgtcgccgaa ggtcgtcggg tcgccgtccg
    18781 ggtcgaccgc ccactcgaaa gcatcaaaga tcggggcccc gtggatatcg atcaccgccg
    18841 ccgagatcca ccgggcgtcc agcgccaccc ccaccgtatc gcccgtggca tcgtcgtgcc
    18901 cgaccagaag gcccatcacg tgcgtgccgt gcccgtcgtt gccgacggtg tgcggaaacg
    18961 gttcctcgtc gagcggataa aaccaggcgg ccgcggcgtt gccgtccaga cctttccatc
    19021 cggaggcgat cgccgggtgc gcgccgtcga cccccgtgtc gaacgagcag atgagccgcc
    19081 cggcgccggt gtacccggcc tgccaggcca gatttgcctt gatcaccgag agattggccc
    19141 cgaccgccgt cgccgatgac ggcgccggtt ccggtgcgat gagagtgagc tcgggcggcg
    19201 ccgccaccaa gtccacgtcg ccgcgccgcg ccgccgcccg gatctgtccc ggcgtcagtt
    19261 cagcttcgat gatgttgccc agccagtgcg ggcggacccg ggcggcctcg ccgcgggctt
    19321 caagcccccg gagaaatccg agcacattcg cctggcgcgc gccgtgggcc tctttgagcc
    19381 gccgcatccc ctcgcggtgc cgggtcgccc catcggggta ggatgaagtc agagcggctt
    19441 tgagagcgcg gggatcgacc gcccccgtca ccccgatcca gaccctgatt ttctctcccg
    19501 ccgctttccc ctgcagccgc tcttccagct ccggcgccag ccggccggcc gacagcatcg
    19561 gcggcgtcag aatccagagg ctcagtgcta tggacagcat ccgcataccg gcgacactca
    19621 tacgacccgt cctcatttca gaatcaactc cctagtgacg cgtccggcgc ccgattgacg
    19681 caaccgaatc gagcgccccc gaccgctgcg gcgcccttgc ggcgccggtg tggggcgggt
    19741 gcctgaacag cccggatgag gactcgccga ccgcccgtcg tcggccgagg cccgaacagc
    19801 ctgggcggcg cggcgtcggc ggccggtctc tcaccgcttg agagcaagat acttcctaag
    19861 cttgcctttg ccaagcggtt tgctgatatt tccgataaac aagagagcga ccactgcctc
    19921 acgaggacac gaaagaccat gaatctcggc cacctctcgc ccgacacgct gcgcgccatg
    19981 ctcgcagatt ttgcgaaaaa ctggctggct catgacggcc tttggttcca ggccgtcgag
    20041 cgccgctatg gattggaaac agcaatcgcc ctcgatgccg aggcctggga gcagttcacc
    20101 gccatcgagg cccgccggat catggccctc catcgccttc ccgaaaacgg cggcctggag
    20161 gctctcaaga ccgcccttgg gttccgcctc tacgccttca tcaacgagca ggaaattctc
    20221 caagaggagc gggcggccga tggctccctg gtctcgttcg agttccagat ggtcgactgc
    20281 cgcgtacagt ccgctcggcg ccggaaaggc cttccccttt tcccctgcaa atcggtcggt
    20341 ctggtcgagt acgagggttt tgcccgcacg atcgatcccc gcatccggac acaatgcctc
    20401 tgctgtccgc cggacgcggc cgccggcgcc gaccagtact gcgcctggcg gttctcgctt
    20461 tagcccgagc ccctgatcgg cgccgaactg cggtctctcc gcgcgttcct caagtgtgac
    20521 cggggggctt caaatggcgg aagagttcgc cccggcgttc ttcagcaccc cgccgggggc
    20581 ggtcccgccc ggaaaagata cgccaccagg tgcgtcaggt ccgccacttc cgtccccccg
    20641 ttcccgttca catcggcgtg ggccggacac cccgcccccg gtccgccccg gaacagatag
    20701 ccgaccagat gagtcacgtc ggccacggtg atgccacccg ccatgtcgac attcccccgg
    20761 gccgtacagc agcaggcgtc gccgatcccg tcgccgtccg catcggcctg gtccggattc
    20821 tccgccatcg ggcagttgtc gcaggcatcc ccccgcccat ccttgtccgc atcgacctga
    20881 tcggcgttgg cgtgatcctg gcagttgtcg caggcgtccc cgattccgtc gccatccgca
    20941 tccttctggt ccggattggc gaccgcggcg cagttgtcgc acccgtaaca ccagccgtcg
    21001 ccgtcggcat cgagcggaca ggcggcccgc tcgacccgga tggaatctct caccggtacg
    21061 ccggccgcca tgccctcggc cacgatcggc agcgctccct ccgccgccgc cagctcgcag
    21121 tacagcccat ccgggagcgc ctcacacatc tgcccgttga ccttcactcc cggcgctgtc
    21181 tgatcggact gaagcaccca caccgcgatc gcggggcaca gcgtcaccgc cccgtcgggc
    21241 ggcgccgcga cacacaggtg gcgcgagttg ctcgtccggt gcgtcagcag cggcaccccg
    21301 gccgccgccg tccccggaat gaccttgagc gagtaggggt agcagtagtc gatgtcttcc
    21361 gatccatcca ccgggcagga ggcgagagcg gcgtagtcgg ggagttctcc gaccatgcgg
    21421 gtcgcgtcga gatacagcca gcggagattc accctcaccg ccgcgaacat gtgctggctg
    21481 taccggaaca tccagcgcgt ctcggcgatc cccaggtcgc ggtccgggag gccggcggtg
    21541 aagagcagcg aggcgagtat gtgcgcccgg ctcatgcacg ttccccaggg caggaatccg
    21601 aactggaaat aggtctcggc catcgcctcc accgagggcc acccgtcgtc gtaacccatg
    21661 aggaaattgt tggcctcggt ccactggggg tggctgccgg cggcgcgggc gttgaccgcc
    21721 agccagtgcc agactctgcg gcaggcctcc cagaccagcg agtcgctcgt cggcgtcgcc
    21781 gccggatcta cgccgatcag ccccacgagc gaatccacgc ttgccgaggg cacccggatg
    21841 tgcgggaaga atccttcctc cgcctccacc tccccgccgc tccgatagca ggccccgccg
    21901 gcgtacgcgt agtgcaccgt cccctgattg atggtcccga ccaggtagcc gtcggcgaag
    21961 gatcgcaggg cgaagtcgtg ggtgaggggc actgcggcca tagtcagcgc ggccgattgg
    22021 tagcccgccg ccgaggccat caccgtgtaa tccgtgccgc tcgccagccc gctcacgagg
    22081 tattccccct ccgcgtcggt ggtgtccgcg accgtcaact ggaccagcga attgcccacc
    22141 cgcaccacgg cgccggggat ggcctcgccg ctcgcctggt cgtacacccg ccccgcgacc
    22201 tcggcggcgg ccgccggcac cccggcggcc agcaccgcgc cgcaaaacaa aagcgcgcgc
    22261 tttccgatac ccgaaacaac catcgcacac ctccgcttga ccgaatcgcc ggcgccccgc
    22321 ccgggtcctc ggaccttccg cccgccgcgc cgggactatc tccaatatag ttcatggatc
    22381 aaaggctcca atagaaaaga agacggcccc ggcgggcgcc ggggccgtgg actcgtgctc
    22441 tctgatttga agtcggagaa ggcggcgctt atttcgcttt gaagaacgcc accgtgcgcc
    22501 gcaacccttc gtcgaacgac acgctcggct tgtacccgaa cgctcccagt ttgtcgatcg
    22561 cggcgaagga gtgtctgata tctcccggcc ggggatcgac gtaggcggcc tgggtgctcg
    22621 tcccgataat gcggcgcagg cggtccagca gctcgttgag cgtgaactgc ccgccgcagg
    22681 cgacgttgaa ataatcccct ttgatccggt cgttggtcgc ggcgaggatg ttggcctgca
    22741 cgcagttgtc gatataggtg aaatcgcgcg actgctcccc gtcgccgaac acggtcggcg
    22801 actcgttccg catcagcgcc gtgatgaagc gcggaaccac cgccgcgtac tggctcgccg
    22861 gatcctgctt cggtccgaaa atgttgaaat accgcagcgc caccgtcgga aacccataca
    22921 gctcccagta gactttgcag tagtactcgc aggtcagttt cgtgatcgca tagggagaca
    22981 gcgggctcgg ccgcatcccc tcgtgcttgg ggagttcctc cgactcgccg tataccgagg
    23041 acgatgacgc catgacgaac ttcttcaccc gggcgttctt cgccgcctcg agcacgttca
    23101 gcgtgccgcc gacattgacc tcgttggacg tgagcgggtt cttcaccgac cggggcaccg
    23161 acggcagcgc cgcctggtgg agcacgtagt cgaccccggc caccgcctgc accaccgtcc
    23221 agtagtcgcg aatgtccccg tcgaccacct ccacgctcgc gggcagatcg gcgagattct
    23281 cctgccggcc ggacgagaaa ttgtccagga tccggacctg gttcccgtcc gccgccagcg
    23341 ccgcggcgag attcgatccg ataaaacccg ctcccccggt cactagaaaa cgcataccat
    23401 cgtcccttct tgacgttgcc gtccttctcc catgactatc ggctgttcgg ctcccgatgt
    23461 tagcgcacca ggcagtagtc ggcagagcct gcctgagaag cagtccaagc tacgggatcg
    23521 gtttcccgct gtaaagcgaa atcacggttc tccggcggcc cgcgccgggt cgactccggc
    23581 gcctccttcc cggccccggc gggaattatc tcaacgaccg tccgcccgcc acattctgtc
    23641 agccctccca ccggggcccg actgcggagg ccgcttggaa gatcctactc tccgccgccc
    23701 cgctcctgcc tgagaatcac gtccgcaagg gcccgggcga taatctcatg cccccggcgg
    23761 gtggggtggc aatggtccac gaacagcccg tcgccgccgt tggcccgaaa ctcctccgcg
    23821 actgtcacca ccgtgacgcc gggcttttcc cggagcgccc aaagcgcctc gaggtacggc
    23881 tgcttgatgc gcagggacat ccagtctgcc tgaagcgccg agtccagcag aacgaaagga
    23941 actgagccga gcgccaggac catggaggta tccgcgagcg agtcgagcag gttgatcgcc
    24001 cgattgaaga ggatgggcga cccggcggcg tcccgttcca ccgcggaagc tgcgtcctgg
    24061 ccggcccagc gccggcgggc cgcggcgaat gcccggtcgg cctcgctcca cgcgcggttt
    24121 cgccacaggg ccacacccag gttgttggcg gcgaccggat cccccggcgc cagccccgcg
    24181 gcctcctgcc aatgcgccgc cgcggctgcg ggatcaagcg gatccgaagc cgccgaccgg
    24241 tacacggccc gggtcatgtg gtccaccgcg gtgtcggtca cgctgaagcg cctgccgtcg
    24301 agacaataga ggatcggccg gtcgagcaga cgcgccagtt gcggtggaaa aacgaattcc
    24361 ccgtcctccc cggtgacgtg gctgaagatt ttgaactgaa gaccggccgg ccagagatag
    24421 cgctgcggcg gcgccaccag caccacccgt gtccgatcgt cccggcatgc ccggatgata
    24481 cgctcgagat tcgccgcgta gcgctccggc gagacccggg gcacgagcgg cgcgccgaga
    24541 gcgccccggt cgcgcacggg ccgcaccagg agatcgcgca gcagccggta cgccgccgaa
    24601 cgggccagca ggcgccgcag gccggccgcc cgctgtgacc ggagcaaatc cagatcggac
    24661 caggaacccg acaccgaggc gtcgttgttg ccgcagtaca caacgacata gtccggctcg
    24721 taccggagaa gctccctctc atagaggcgg gcgagctgct cgctggtgta cccggtgacc
    24781 gaggcgttga tcatctccac gcgcacgtcc ggatcaaggg ccgcggccag aaggcgggtc
    24841 aacacctcgg gaaaggcgaa ggtgcggtct ttcagaccga ggccgaccgg cgaggagtcg
    24901 ccgagcacca tcacccgcag cgtgcccgcc ggccttgcgt acggtacctc atcggcgatg
    24961 aggccgcggg aattggtcct gatgagggga ttgtgcaggt tggggcgaaa ccgccagagg
    25021 agatccgggt cgggctcgtt ccaggtgaag agcgatcgga agaagaccgc ctgccagccg
    25081 atacccccgg cgggcatcgc gtgagaatcg agttgcgagg tcagcaaccg ggcgccgcct
    25141 tcgatcagcc cgagcagaac gaccgtcgtc acggcggcat acatgagccg ccgcctccac
    25201 ggcagcggcg gggcgcttgg tgctctcatg atagacaaac ctagcgcaca atcgtgccgt
    25261 tgtcaaaggg tgcggccggc cggattcgac aagcgcccgc gctccctccg ggccggaagt
    25321 cgcgggcgtg ttttccgacc ctgacgggcc ttatgaagcc agtgcatttg agcctcgcgc
    25381 cggcccgcgc ccgcgtgccg ctattttcgc agaccctcca gccaactccg cagcttctgt
    25441 tcctgccgct cgggccggta tccgaccacg ccgaccagct tctgggccgc cataccggcg
    25501 atgattcgcc cgtagcgcgg gtgttccaca tatacggccg attccggctc gaacggcagc
    25561 accgccggga ccgtgagccg ccgggtcccg tcctcggccc agtcccgcag caccacgagt
    25621 ttcagtccgc tcgtatcagt cgtcaagaac caccgcgcgg tgtcgattcc cgccacaaag
    25681 tccatcgtgt agacgtcatt gagccggggc gcgccgagga aaccgtagcc gacgtaccgg
    25741 tcggaggccg ccacccgatc gtgcatggga aacagaatgt gataggggga cagacgcatc
    25801 tcgcccgtcg cccggttcgc cgcctcgcgc gcgagccgct cggccgccgc gcgggccgcg
    25861 gccgaggtgt ccccggcctc ggcgacgatc gcgtagctcc ccttgtacac gtagagcctc
    25921 gtccccgtcc agaacgactg ccgcccgatc gtgtcggtcg ccgccccgtt gggccggtgc
    25981 gcggcatacc acccataggc cgcgatcggc tcggcaaact gggcgatcgt cacccgtacc
    26041 tccgcctcgt ccgccgtgta ccgcgccgcc gcttccccga tgaagtcgta ggccaggtat
    26101 cccggatccg agctgctgtc aaccgcgagc gcgccgattt tccggaccgt gggcggctcc
    26161 tccggtcctg ccccgccgcc cggcagcagc gagtcggcat tgaacggaaa ttcttccaga
    26221 tgcgcgatcg gcggccccgc cggaaagagc ctggctgccc gctcctgggc gtcatccccg
    26281 caggccccga gccagagcgc cgccgcggcc gccggcccgg ccgcccacct cacaaaacgt
    26341 agagcacgaa acttgccgtc accagccata acatcaccgt gagaagaatc ggacggtcgt
    26401 cgatcagcac ccgcgtcggc gatcccccct ggtccttccg gtggatcagg tagagatacc
    26461 ggaagacgcc gtagacgaca aacgggatcg tcacgaacag cttgtccgtc ccgagcttgt
    26521 gcatcacttc cgtcgaaaaa gtgtagagca tataaatgac cagcatcgcc gcggtgacca
    26581 ccgcaatcaa ctggtccagc aggtagggcg agtacatcac cagcgttttc cggtgcgcct
    26641 gcgcctcctt gtccaggtag agcagttcgt gcctccgctt tcccagcccg agaaacagcg
    26701 ccaaaagcag ggtgttgatg aggaaccagg tcgaggcggg cacgtcgatc gcaaacgccc
    26761 cggcgtaggc ccggaccacg aaccccagag cgatcgtgag gacatcgagg atcaccacgt
    26821 gcttccacca caggctgtac aggagattga gcgccaggaa cgtgaccgcg gacaagaaga
    26881 acgggccgtt gatgaaccac gctccgacca gcccggcggc ggccagcacc cccgccagca
    26941 ccgcccccgt cgccacccgg atccgcccgg ccgccagcgg ccggttcttt ttcaacgggt
    27001 gcaggcggtc ccgctccgcg tccacgacat cgttgaacgt gtagacggcc gaggacaaca
    27061 ggcagtagag ggcgatcgcg caggccatga gaatgatctt gtccgcgtgc gccaattccc
    27121 cggcgaacac gatcgccagc accatgatcc cgttcttgag ccactgcgcg ggacgaacga
    27181 gcctgaggat atcgtggatc atctcgacga aggatacgac aaggcaaaat ccggttcaag
    27241 ttgaaactga ccggccggga cggcccccgc tccgcccccg gttattttct tttgcttgac
    27301 cgcccctttg cctatactta ccgcaaatgc gaaaacgaca gcttaaaggc gccggcggcc
    27361 attacctgat cgcagtgaac cagcaggccg ggcagttttc cgaccgcctc ctgcgccggc
    27421 tcacgggcgc cattcggagt caggggagtc actacacggt tatcgagccg gcctcggccg
    27481 ccgatctggg agcgacggcg cgcgagtact gcgggctggc caaggccgcc cgcccggcgc
    27541 cgcccccggt ggccaaacgg ggccgggtga ccgccctggt cgccgccggc ggtgacggca
    27601 ccgtcaacgc cctcgccgcc gtg
//
LOCUS       DAOPOE010000036        27455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2235_length_27455_cov_5.936387, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000036 DAOPOE010000000
VERSION     DAOPOE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27455
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_27455_cov_5.936387"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..347
                     /locus_tag="PKM94_06525"
     CDS             6..347
                     /locus_tag="PKM94_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD66333.1"
                     /translation="MIKHHELFKGLGDPTRLRIVALLLVRELCVCDLMAVLRLPQSTV
                     SRHMIRLKGAGLVQDRREGKWVHYRLKSNPIVSDLRRFLKSHVTDAEQFRKDAAGLAR
                     YSRTKRGAPAQ"
     gene            356..784
                     /locus_tag="PKM94_06530"
     CDS             356..784
                     /locus_tag="PKM94_06530"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOD66334.1"
                     /translation="MRILVLCTGNSCRSQIAEGLFRHYGGGQVGVYSAGLEPKGVNPR
                     AVQVMREIGIDIAGHTSDHLSQYLERSFDFVITVCDNAATNCPVFPGAATKLHWPFAD
                     PANATGSEEEITASFRSVRDHIELTVKRWLSEALGRGEGA"
     gene            complement(987..1060)
                     /locus_tag="PKM94_06535"
     tRNA            complement(987..1060)
                     /locus_tag="PKM94_06535"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1024..1026),aa:His,seq:gtg)
     gene            complement(1235..1489)
                     /locus_tag="PKM94_06540"
     CDS             complement(1235..1489)
                     /locus_tag="PKM94_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66335.1"
                     /translation="MIKAFVAVIGVIFLMGLLGLGGIGLLLGLIGGLFGLIAGILGAA
                     LGLVVGLFGVVFGTIGGLVGILLPVAAVVVILAGLAHLLA"
     gene            complement(1619..2101)
                     /locus_tag="PKM94_06545"
     CDS             complement(1619..2101)
                     /locus_tag="PKM94_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOD66336.1"
                     /translation="MPRRYWLMKSEPNRFSIDDLRAAPQGIAPWDGARNYQARNLLRD
                     RVKAGDGVLFYHSGTATPGIAGIAEVVRAGYPDHTAWEANGEQFDPKSTPARPVWYMV
                     DVRFVEKFRQLIPLAKLKTTPGLEGMEVARRGSRLSVQPVSRSEWEIVRRIARNMDPG
                     "
     gene            2216..2875
                     /locus_tag="PKM94_06550"
     CDS             2216..2875
                     /locus_tag="PKM94_06550"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOD66337.1"
                     /translation="MADRTKEEKYALLRSRMVDEQLVRRGISDPGVLAAMRAVPRHLF
                     IPKKYRADAYRDSPVPIGSGQTISQPYIVASMTEELALDHASTVLEIGTGCGYQTAVL
                     AEIAGRVYSIERLPDLHELACATLGLLRYDRVNLRLGDGYRGWAEEAPFDGIIVTAAA
                     PRIPERLTGQLSVGGRLILPVGSEFGRQELVLIERTATGLVEKVLYEVRFVPMQGDSQ
                     A"
     gene            3264..3662
                     /locus_tag="PKM94_06555"
     CDS             3264..3662
                     /locus_tag="PKM94_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD66338.1"
                     /translation="MRMKYIQKQSGLSLLEVLIGMIVLAMGLLGLAPMLVMSIEGNVM
                     SRDNTVAANLAKQQVEQYEGMNSLPAIPFTLQETGLAGGRYTRSTYIRGHATDASIPT
                     GVYQIDVTIAWTDNQNVPRTTQYSTFILEP"
     gene            3682..4245
                     /locus_tag="PKM94_06560"
     CDS             3682..4245
                     /locus_tag="PKM94_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD66339.1"
                     /translation="MYRPLRHSAGYTIMELLVALVLTGIVAAAGFSFYVSMHNSALTQ
                     EDISNMQHTSRSSLQEIAKTLRMAGYKLAGHAAYRISGDSLYVFFSDTQPVDTVLYYL
                     QENTALGVNAPNGWKPMFLMKKVNNQPAAVFSDVVRSITYTVIDSANVQVSIEAQTAK
                     SDESWDEDSGYRSFTATKRVTIRNLEL"
     gene            4259..5428
                     /locus_tag="PKM94_06565"
     CDS             4259..5428
                     /locus_tag="PKM94_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOD66340.1"
                     /translation="MKAKLQTIRGSALLIALLLTGMLSLIAIMAIESSNTDIELSYSQ
                     SHLDAAFYNAEAGAKRAFVALNDNDDWRSGYADFGFGQGTFSVNLFDSLTTPSLADTV
                     VIRSRGDVREAQAEVEMWTVPVYINPFVWAMFADSAITMDQTTCTDSYNSDSGAYAAT
                     VLQDEGSIGTNGRISTSKTVTIGGDAKTAVGGSITLGAGSQVLGDTSTTQDSVNIDII
                     PQSEYDWARTNSLAPLGLSGTGWSYNNGTRKLTMGSYSSVVLQSGVYYFSEIVMGQGS
                     TISLAPGAQVRIYMSGNIDFNQNGRVNASGAPQNLQIYSQGNLFQLDQGNIFCGTFFG
                     PNAHIQYDQTTQVYGALVGNSIKLDKGACFHYDRNLAKIRHGTTGEMIQVAWKEL"
     gene            5533..6690
                     /locus_tag="PKM94_06570"
     CDS             5533..6690
                     /locus_tag="PKM94_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOD66341.1"
                     /translation="MDKPFTTERGSALLIALLLTGMLTLVAFIAMDNSNTDIALSYNV
                     MNSDRAFYAAEAGVKRSLVALNADPSWNEGFADVPIGAGSFSVAVEDSSVTPALADTI
                     IVTSTGLVRDAQSTIEMTLVPVRFNPFLKALFGDNSVDIKNSMESDSYNSDSGAYAGT
                     RTWHDGDVGSNGTITIANGAIVGGGVATSQPGGLSINAGATVYDDTTSTAPRQEVPDV
                     PQTEFDWAAAHNQNLSGISGSFTYNPSTKAFETSGSVTLSSGVYYFSSIILKNSASME
                     VAPGATVMIYVTGDIEMKNSAAMNTAGAPKDLQIYSQGDIVLKNSGDIHAVFYSAGGE
                     ADLRNSGQFFGAVVARDIVVHNSAKFHYDRSLGEIEKNWDAHMKVVAWREL"
     gene            complement(6781..9258)
                     /locus_tag="PKM94_06575"
     CDS             complement(6781..9258)
                     /locus_tag="PKM94_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD66342.1"
                     /translation="MSRPLRTACALFLLLALLGPATGPAAQENITYQTPPQAIVDIVD
                     APRTPRLSLGPDRQNLLLLEYPALPPLEEVAQPELRLAGERINPRTNGPSRGGYYSSM
                     KLRTLDDRTEYPITGLPSPVRITDIEWSPDGSRVAFACTIEDRIELWLLDLAARTARR
                     LSERPLNAVFGSPFTWFSDSRTLLVRFLPDDRGEPPVKPRVPEGPNVQENLGAKAPAR
                     TYQDLLANPCDEQLFDYYGAAQTALVTVDGAVTPVGRTGVLVNTSPSPDGRYILAETI
                     HRPYSYTVPAERFPRTIEVWDRAGATVYRVADLPLADRVPITFGSAPTGPRDVDWRAD
                     ADAALFWVEALDGGDAAVVTDERDRLFLLPAPFTGEPAALLTLGFRYSGVQWGNDSLA
                     LVNETWWPTRTVRTWRLRPGRPEARAELIVDRSWEDRYQDPGAPMLDRSGRGTYVLAT
                     ADTGTVIFRTGAGASPEGNQPFLDAFNVDTKETTRLFRSAAPYYEEPVDFLDIDRRLV
                     LTRRESVDQPPNYFRRDLRHDDLMQITFFPHPTPQLRDVKKELVTYTRADGVQLSGKL
                     YLPPRYDPKRDGPLPMLMWAYPEEFKSAGAAGQVTDSPYRFVRASWSSPLLWLALGYA
                     VLDDPSMPIVGEGDAEPNDTYVEQLVASARAAVDEMVRRGVADRDRIAVGGHSYGAFM
                     TANLLAHSDLFAAGIARSGAYNRTLTPFGFQSEERTFWEAPQTYFTMSPFMQADRINE
                     PLLLIHGEADNNPGTYPMQSERFYAALKGQGATVRLVMLPHESHGYQARESVLHVLWE
                     MTQWLDTYVKNRRLAEPGAVKSMSGAE"
     gene            9394..10218
                     /locus_tag="PKM94_06580"
     CDS             9394..10218
                     /locus_tag="PKM94_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOD66343.1"
                     /translation="MMRTACYMSRAGCALAALSVGAALSAGCSKSTDAEPPPAPDSTR
                     TYTYTVIDSFPHDSTAYTQGLAFGPEGYLYEGTGLYGGSSLRRVELTTGRVDTMIGLA
                     ALYFGEGIALLGNRIVQLTWRENSAFVWNRASFAREGQFSYAGEGWGLTFDGSRFIMS
                     NGGSALQFRDTATFEIRRSAQVRDDDGPVDRLNELEYIDGEVWANIWLTDWIARIDPD
                     DGRVTGYVDLTGLLSRTAHPTAEVLNGIAWDSAGARLFVTGKYWPWLYQIEVREAP"
     gene            10215..11024
                     /locus_tag="PKM94_06585"
     CDS             10215..11024
                     /locus_tag="PKM94_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOD66344.1"
                     /translation="MTAGPGRPLRGAVIAALLAVLPAACGSSQKQPAPPAKIAAYRVK
                     VIRTYPHDSLAFTQGLAYEGGYLYEGTGKPGASSVRRVRLETGAVLQRRDLPAPHFGE
                     GIALVGDRLVQLTWTSRTGFVYDRATLAPLSEFRYLTEGWGLTYDGIRLIMSDGSATL
                     QLLDPQTYEYCSFLAVRADGEPVERLNELEYVDGEIFANVWPGDRIARISAATGQVTG
                     WIDASGLLAPEDYGDSTDVLNGIAWDAEHRRLFLTGKCWPKLFEVELEPVE"
     gene            11043..11600
                     /locus_tag="PKM94_06590"
     CDS             11043..11600
                     /locus_tag="PKM94_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66345.1"
                     /translation="MRAGKSIATLRLELIPATPSLLAADVARSARLAEELGAVVPPDW
                     PPSELAEVLPRLLSALERRPDLRGWWAYYWVASGEGRGPRTLIGSGGFHGRPDAEGCI
                     EIGYGVLPDFQGRGYATEAVAALVAWALRHRTVTMVFANTGADNQPSIRLLERLGFLR
                     AGIGAEPWTVRFEVDRRRFRRPMPR"
     gene            complement(11608..12024)
                     /locus_tag="PKM94_06595"
     CDS             complement(11608..12024)
                     /locus_tag="PKM94_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66346.1"
                     /translation="MVIEYVDSAAGLAPDRLTGFFAGWPNPPSPEMHRRILRRSDCVV
                     LAIDPSAGRVVGFINALTDRVLSAYIPLLEVLPEYRGQGIGSELVRRLLKRLSGLYMI
                     DLMCDPSVQPSYERLGFVRAAGMSRRDFSAQGGKPD"
     gene            complement(12028..12555)
                     /locus_tag="PKM94_06600"
     CDS             complement(12028..12555)
                     /locus_tag="PKM94_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66347.1"
                     /translation="MDSYVAWVTARPLLSAALQFALLGTLGEIVSVSIRRGRPALPGT
                     WWETLAKVPAWAVLGIVIKYGFTGMKGFTAALLDHHFLPAACGAGLGWAFAVSVLTNL
                     LFGPQMMFFHRLEDNLILRRWDMTGLTTAWWTLLWFWIPAHTLTFSLPTEYQIGLAAL
                     WSFVLGIILSFAKGR"
     gene            12873..16124
                     /locus_tag="PKM94_06605"
     CDS             12873..16124
                     /locus_tag="PKM94_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66348.1"
                     /translation="MIDPLINWPTYYGQQNTGLVRATFGDNGSFGWSFLRRTMGIPGW
                     PEAGFEVPVSSGVDYLFGGAVWIGGVVGSDTLVSTGSEGWVNVREFFPDDLAGAVDEL
                     FGPADFAMRAEFTDTSVARVSVTDEFDSRLHLPLDVRITNRSHAWRTSPADAAVVYDL
                     VVTNIGVEAVHDGYIGFYFDSDVCGDCDANSGFADDLSGSLREQGIGYVIDNDGDSLP
                     GETPRAFCFKLLHSSVPLPDTSFNWWASSAIAELDFGPRMKDRPGDPYRDFGTGGTGT
                     AVGDANRYYLMRHQEWDYDQVWCHSIGPDDTLWWQPPLSLAYDLADGFDARFLMSFGP
                     FDLAPGQSERFIIATFTTDSIHTLPGNAANLPGDPAAYLANLDFTSMIRNANVADSLA
                     AILVDPLQPPAGLQVRATSGNDVTIGWDGSCLPEVTGHVVYLSVVDPASLPYPGVAPP
                     WLEMTELEIEATPGPEQSAWTLPNLATDRFYLASVSYRSASGTGEPAHPITVWAGERA
                     EAPEFVGSEYVFVAPGDNIPLSWTTLSSMAVDHFNLYRFSTPQDAENAYHAFYDRGFA
                     ASAIAPADSFDVNGRRYYYYAMSPYQVLPGAATLFVDEPVAESTTYIVAAVDPAGFES
                     HFSEPVEAVHVPPRDKDILVLTHGSLAGVQFQSLSRVVEFYDSLLTGYDYDLYDWGDS
                     NRIYIETDWHLLMEYNLVVIDDDLRDGIFRPVWEDTTQAFTRYLLSGGRLAYFGSGGS
                     MAGVDAITGAPSTRNLTHSLVRRFFGAESGRIVPAIYLAALYHFPPSYADTIFGFQRA
                     DPAALEYPALTYDLASGRINPGALQLWPTNAPLSVSTFAPTAAGEVTHLFGADAAINS
                     TMAGEPVGIRRQVGPAETYLFGFHLWYLELPGARALIDGMFDRAVLARFDPDTVLMVS
                     AWSLEPETLTVHVGNLPSGLSVYDIESGSVRLSETVVPLSTEYTSGVPGFVGDVLAVR
                     VSAADLLSLYGQVYGYFRIPIKVSGSFAGGDPFAVYAGLVLLGKLVGDYDWNGKVNIA
                     DITMGVDRLFRGAYPELAPEDMDVSGDCRFGVADLAVLISYVFRDGPPLMRCP"
     gene            complement(16510..19605)
                     /locus_tag="PKM94_06610"
     CDS             complement(16510..19605)
                     /locus_tag="PKM94_06610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOD66349.1"
                     /translation="MTSVPYIELHCHSNFSFLDGASDPEALVDRAVELGYPALAITDT
                     NGLYGIVRFDQQARRAGLRPIFGAAALLDTGHRLVLLVKDGAGYANLSELLSTAQLES
                     PKGEARVSRALLERHADGLIALVHDDLAACLLREEHSEAERLLADYRDLYGRDHVYVE
                     LRHHNLPVHEHLCAALVRLGRRLGLPAVAANDVHYAAPDGRRLQDVLTCIRHHTTLDE
                     AGELLYPNAERCLQPPAVMARRFARYPEAAAHTLAIAERCWFSLEQLETSLPDFPVPA
                     GHTEQSYLEELTWAGARRRYPEMAEPHVRQLRHELRIIDRLKLAGYFLIVWDIARFCA
                     ERAILCQGRGSAANSAVCYCLGITAVDPIKLDLLFERFISEERREPPDIDIDIANNRR
                     EEVIQYVYAKYGRTHAAMVCEVISYRGRSAVRDIGKTLGFSPDEVTRLSKLLDHWASP
                     EDVAERLREAALNLGDRRVRLLLDLCRQIHRFPRHLGIHVGGMIITRKPLSQVVPIEN
                     ATMPDRSVIQWDKDDVGAAGLVKIDLLGLGMLTLIDLALKLVKEHRGVTIDPARLSYD
                     DPKVFDLLCRAETLGVFQVESRVQMNALPRHRPRCFYDLVIEVALIRPGPIQGDMVHP
                     YLRRRNGEEPVTYPHPCLEPILRRTLGVPLFQEQGMKVAVAAAGFTASQADELRRAMG
                     HKRSRERMAALAERLIDGMQRHGIPHDAARRIFDQLAAFADFGFAESHAASFALLVYV
                     SAYLKVYYPQEFYCSLLNAQPMGFYAPSSIVYEARRRGVMILPVDVSRSRFDSTVEGD
                     AVRLGLRTVRHIGETARATIESEQARGPFTSLKDFVRRSRLGPQPLAQLARVGAFDCF
                     GYHRRQALWIVLGLSRRAGELDLAVEETGHTTLRPMHIGEQIVADFKGMDLSTGPHPM
                     ALIRDQLAACKILAAADLRHRRDKSRVAVAGVVVIRQRPVTAKGFLFLTMEDETGFIN
                     VVVMPSMLARHRRTIVHHQALIVQGILERQDGVINVIGRQFAPLEAAPGDVTFRSRDF
                     R"
     gene            19604..20212
                     /locus_tag="PKM94_06615"
     CDS             19604..20212
                     /locus_tag="PKM94_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66350.1"
                     /translation="MDLLGQAPCGPDILAIDEDDPVRTIKVLTYKKNLLTEVSLKVIR
                     FYISPTLVIKSSLGEPCQGGVPVVERVAYEAVFDRVIMDIVYMALQICRTADDMIPVA
                     MLPQAALAHMMSSPIECGEILFHAMDDYIGLSIPRLQNSMPVLGLNYPRQYSVFQDHA
                     GSPDSGQQQIRAVEENGLPVVCNAGDEICPARFEETTQFRHR"
     gene            complement(20312..21835)
                     /locus_tag="PKM94_06620"
     CDS             complement(20312..21835)
                     /locus_tag="PKM94_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOD66351.1"
                     /translation="MNERRLACIHLPDFPLVLYSRLHPEVRAHPLVLTTAEGETATVT
                     ICNARAAQYDVAAGMTVARANSLCPGLVIGVRECGRETAVLREIGGALAGVGPEIEIE
                     TGGVIFLESARLGKLYGDEPGLARRIQAVLQPFGYPVAIGFAANKFVALTAARQAAPG
                     GWVIVPSGSESAWLQQLQVEHLALSDDTAETLSDLGIATVGQAAALPANEVVERFGAE
                     GAVLARRARGDDPEPFTPDRPAADWCATAGLDSPVRDAATILAHIEALLKHLLAQPGC
                     AGCAVREIQVCLHLDDRTEQVFTAAVEQPSAAAARFLRQVRLALERMSPAAAVRDITL
                     RIPHPAAAAAEQTAFAQPGARGEIDAETAATIRRTLSGNRLCTARLLPSHLPERAFRL
                     SPVTPSPAKRPSASSLSAGAAPLDRLPPYSLRRIAGLRLLQPPQPAEVHPADGWLRDG
                     SGKHRVTGRRGPWKLSGDWWQTGFDRLYYELQTEEHRQYLVFFDRSRARWFVQGVFD"
     gene            complement(21828..22484)
                     /locus_tag="PKM94_06625"
     CDS             complement(21828..22484)
                     /locus_tag="PKM94_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOD66352.1"
                     /translation="MIAPGDIPQDLAALKRLIQRKWPEAVGLASERLDLAFRTGLPAL
                     DALFPHEGIPCGQLIEITGGPSSGKTTLLLTLLASLSRSGRAVYIDLSRSFFPAAAAA
                     CGVDLERLLTVAPANGPTALRVAELLLQSRIASWIVLDLVGQKEPLPGLMLHRLRLKV
                     SRSRGLVLLLTENNSGVIPPSMASLRLAVARAGRGVRLTVARSRICKEGESVEVCFDE
                     "
     gene            22758..24431
                     /locus_tag="PKM94_06630"
     CDS             22758..24431
                     /locus_tag="PKM94_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOD66353.1"
                     /translation="MSSKHLLAAMTVAFLMPVLAAASAEDNSTVIDSRIDRVTVYPDR
                     AGVVRLSTLNLPAGEHSILFEGLPIGIDPASFRSSARGPRGTMVLGLTHETVAHLDAP
                     QARAAELERQIQTLERETRQALQVRLEVLNRQRDLLAALAKEHGEEPDRNGGVGRLDM
                     ANWAAAYEFLSRHLVQVGDSVRLVTIALEDADRQLALLREQLDKLRADQGRETRTVRV
                     DLALAQAGTVEIELQYTVAGAGWRPIYDARLRTAADSVELACFGEVIQRTGEDWENVA
                     LTLSTAIPSQGMEPAAFRPFFLSVLSDAYEAKRQRNTALEAPAPAMMDMAAMGKAEQE
                     LAEAVNAAAIINAGGLATTFTAARREDVPCDGRGVRAPIAQWTFGGTLALISRPRFAD
                     GAYRRVTMTNTSDAPFLPGRVAVFAGSDFLGSTAFTKFIAAGETFDLPFGRDNDVTVE
                     RKIVRHEVGDGSTWLGIGGNRRRVSETVAITIKNNGRRARTITIEEPLPVSQDDRIEV
                     SLKDFSRKPDDQDESGRAIWTVTVNPGAEDRLTYRYEIVHPADVIISGI"
     gene            24608..25684
                     /locus_tag="PKM94_06635"
     CDS             24608..25684
                     /locus_tag="PKM94_06635"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD66354.1"
                     /translation="MEQPSARQTAPPKPARRPRYVRRDRSDNIQKWRFAAQLFSTAVS
                     LWIGVQFYLWTKKLEGAALWSWVPRPPGVEGWLPIGAIVSLRYWIESHVFNYVHPAGL
                     VIFLFILGSSWLFKKAFCSWVCPVGFISEMLGDFSKRFLRLRIRPWKVLDWPLRMIKY
                     LLLGFFVWAILIQMTPASIEAFVYSNYNQVADILMLRFFTDISRFALAVTGALVLLSL
                     VVRGFWCRYLCPYGALLGLIGLISPTRIRRNETTCIDCARCAKACPAFIKVDKVKYVV
                     SDECVGCMACVDSCPVKDALTVHMFTKKRPVNKRYWALALVVLFWVGLAGFKLFGPWE
                     NNISDAQYREHLPRLERGEYVHPR"
     gene            25731..26492
                     /locus_tag="PKM94_06640"
     CDS             25731..26492
                     /locus_tag="PKM94_06640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66355.1"
                     /translation="MNESFPSVFEVYAHEYDLITNAAQREPHHAAEVAAMIARFAPAS
                     VLDAGCATGLTTLLFARQGVAAVGLDRSPRILAVARQTRCPEGLPISYRRGTFEDLPK
                     SLRNRFDLVVCLANAIAGVGTVARLNLALRNFSAALRPGGHLVLQMLNFAVVKENTLL
                     PVKATHNDGLVYERFSERRGKTLAIYVTRADFSRTPPTFEVFRHSFGSFTPEQVTGSI
                     ARAGFTDLARFGDLLFTRRFSRRSRDLVIAARRPA"
     gene            26537..>27455
                     /locus_tag="PKM94_06645"
     CDS             26537..>27455
                     /locus_tag="PKM94_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66356.1"
                     /translation="MKNLWWTLAAVLLGAGAASAQRAVAPRPSLEAFRPVGEYRLWTF
                     IQKDTVLGTIASTVERTVEIGGLRGYALREDFLLDFTKRSGENRFAAAGEHFVGADGA
                     YLGDDLKLNVNGQEGRINVKRKGDVIRGLVGQGRQRREATVTVPSGMFAIDNYMADQF
                     EIYFALRGLTAGETIDDSIFVPRDLLAAPLRGRVTNFSWVQLYKGVFDSVFTVQLDQP
                     VPVELYINRELHLRKLNYRAQDMRVYLDVVREQAELVKPRLTLGALARMIPKYLVFAL
                     IGLVGLVLYAGSAVRRLTLWLGALTGAVGF"
BASE COUNT         4834 a         8889 c         9015 g         4717 t
ORIGIN      
        1 gcgccgtgat caagcaccac gagctgttca aggggctcgg cgaccccacc cggctgcgca
       61 tcgtcgctct cctgctggtg cgggagctgt gcgtgtgcga tctcatggcg gtgctgcgat
      121 tgccgcagtc taccgtatcc cggcatatga tccgcctgaa gggcgccggt ttggttcagg
      181 atcgccggga agggaagtgg gtgcactacc gcctcaaatc gaacccgatc gtcagcgacc
      241 tgcgccggtt tctaaaaagc cacgtgaccg acgccgaaca attcaggaaa gacgccgccg
      301 gtttggcgcg gtacagccgg accaagcgcg gtgcaccggc acaatgagcg ggaagatgcg
      361 catcttggtc ctctgcacgg ggaactcctg ccggtctcag attgccgagg gactcttccg
      421 ccattacggc ggcgggcaag tcggggtcta ttcggccgga ctggagccca agggggtgaa
      481 tccgcgagcc gtccaggtta tgcgcgagat cggcatcgac atcgcgggcc acacgtccga
      541 ccacctgtcg cagtatctgg aacggtcgtt cgatttcgtc atcacggtgt gcgacaacgc
      601 ggccaccaac tgccccgtct tccccggcgc cgcgacaaag ttgcactggc cgtttgccga
      661 cccggcgaac gcgaccggct cggaggaaga gatcaccgca tccttccgca gcgttcggga
      721 ccacatcgaa ctgaccgtga agagatggct tagcgaggcg ctgggcagag gtgagggcgc
      781 ttgacggact cgcgagcccg ctcgcgacag cggtgagggt tgagccgggc accgccccgg
      841 cggattccgc gtgtggatct gtgcggcgtc tggtgatgac gctcagagct atcctctgat
      901 ttttccccac tcaaccgcct tgaccgtcgc gccgtcccga ccgaacaagg gaggccgcca
      961 aggtgcggga gtgggcagcg agtacatacc ggggggcagg atcgaactgc cgacctcctg
     1021 atccacagtc aggcgctcta accagctgag ctaccccggc atgggcgccg acaaggtagc
     1081 gctttttgac ccgatgtcaa ggaaattgga gtcccgcgat cgttcccccc gcgggcgctc
     1141 gccccgcaat cgttcgtcgc gcgggcgctc gtgccgcggg cgcccggccg ccgcggcggc
     1201 cccgcaggcg cgcaccgcaa ttgtcggacc cgcttcaggc cagcaggtgg gccaggccgg
     1261 ccaaaatcac aactaccgcc gccaccggca agaggatacc gacaagcccg ccgatcgtgc
     1321 cgaagacgac tccgaacagg ccgaccacca ggccgagagc cgcgccgagg atccccgcga
     1381 tcaggccgaa cagcccgcca atcaggccga gcaacagccc gatcccgccg agcccaagca
     1441 atcccatcag gaagatgact ccgattaccg ccacaaacgc cttgatcacg tctcgttcct
     1501 cctcatcctt cggaccagcc agccactctt cactacgtta tccggccgtc gaggttgcat
     1561 ccgcacggag ccgccggcgc tgccgtcaac gaggattacg cccgagcggc cggactattc
     1621 acccgggatc catgttccgg gcgattctcc gtacaatctc ccactccgat ctcgacaccg
     1681 gctggaccga caggcgggag ccgcggcgag caacctccat gccttcaagc ccgggcgtcg
     1741 ttttcaactt cgccagcggg atgagctgcc gaaacttctc cacgaaccgc acatcgacca
     1801 tgtaccagac cgggcgcgcc ggagtcgatt tgggatcaaa ttgctcgcca ttggcctccc
     1861 aggccgtgtg atcgggataa ccggcccgca ccacctcagc gatccccgcg attccggggg
     1921 tggccgtgcc gctgtgatag aagagaactc cgtcaccggc tttcacgcgg tcgcggagga
     1981 gatttcgggc ctggtagttg cgggcaccgt cccagggagc gatcccctgc ggtgcggcgc
     2041 gaaggtcatc gatggagaag cggttcggtt ccgatttcat cagccagtat cggcggggca
     2101 tctttttgcg tcctttctct gttgagcgtc caccgccatc cagtatattg cagccgcctc
     2161 gggccggcaa gtcggtcggc cctggcggcg gcttgcgcct tcggagagtg acgcaatggc
     2221 ggatcgaacc aaggaagaga agtacgcgct gctgcgcagc cggatggtcg atgagcagct
     2281 ggtgcggcgc ggtatcagcg atcccggggt gctggccgcc atgcgggcgg tgccgcggca
     2341 cctgttcatt cccaagaagt accgggccga cgcctaccgc gacagccccg tgccgatcgg
     2401 ctccgggcag actatctcgc agccctacat cgtggcctcg atgaccgagg aactggcgct
     2461 cgaccacgca agcacggtgc ttgaaatagg gaccggctgc ggctaccaga ccgctgtcct
     2521 ggccgagatc gccggccgcg tctattcgat cgagcggttg cccgatctcc acgagctggc
     2581 ctgcgccacc ctcggtctgc tccgctacga ccgcgtgaac ctgcgtctcg gcgatggtta
     2641 ccggggctgg gcggaggagg cgccgtttga cggcatcatc gtcaccgcgg ccgctccgcg
     2701 gatcccggag cggttgacgg gtcagttgtc ggtcgggggg cggctcattc tgccggtcgg
     2761 atccgaattc ggccggcagg agctggtcct catcgagagg acggccaccg gcttggtgga
     2821 gaaagtcctc tacgaagtga gatttgtacc gatgcagggg gatagccagg cctagcggcg
     2881 gtcccgcccc cctgggatct tccgaagtcc tgccgctgct tttcgtccac accgaccctt
     2941 caagctaagc ctcctttgag aacgtcccct tgagccccat gggcctcgat cgcgactggg
     3001 ccggaatgct cccacgatcg cccggcttct cggccacggg cttcctgatc tcctgcgctc
     3061 ccgggggatg ggctgagctc cggcagagta ttgggaacct cccccatctt ggccgggcgc
     3121 ggggcggccg gggcgataaa tcgcccggcg gttcgtattg tccgccaata ggttgcagcc
     3181 cgacgagcag tcccgggaac gggtcggagc gtttggcagc cgttttgcgg tggtccaggg
     3241 aaaagacaaa ggagtctgcg gctatgagaa tgaaatacat tcagaaacag agcggcctgt
     3301 cgctgctgga agttctcatc ggcatgatcg tgctggcgat gggtctgctg ggtctcgccc
     3361 cgatgctggt catgtcgatt gaggggaacg tgatgtcccg ggacaacacc gtggccgcca
     3421 acctggccaa gcagcaggtg gaacagtacg aagggatgaa ctcgctcccg gcgattccgt
     3481 ttacgctgca ggagacgggc ctcgccggcg ggcggtacac ccgctcgacc tatatccgcg
     3541 ggcacgccac cgacgcctcc atccccacgg gggtctacca gatcgacgtg acgatcgcgt
     3601 ggacagacaa ccagaacgtg ccgcgcacca cgcagtactc cacgtttatt ctcgaaccat
     3661 gacggggaag gacggaaggc aatgtataga ccacttcgac acagcgcggg ctacacgatc
     3721 atggaactgc tcgtggccct ggtgctcacg ggcattgtgg cggcggccgg tttcagcttc
     3781 tacgtctcca tgcacaactc ggcgctgacg caggaggaca tctccaacat gcagcatacg
     3841 tcgcggtcga gcctgcagga gatcgccaag acgctgcgca tggccgggta caagctggcc
     3901 gggcacgcgg cctaccggat cagcggcgac agcctgtacg tcttcttcag cgacacccag
     3961 ccggtggaca cggtgctgta ctacctgcag gagaacaccg cgctcggggt caacgcgccg
     4021 aacgggtgga agccgatgtt cctgatgaag aaggtgaaca atcagcccgc cgcagtcttc
     4081 tccgatgtcg tgcgctcgat tacctacacc gtcatcgact cggccaacgt ccaggtctcg
     4141 atcgaggcgc agacggcgaa gtccgacgag tcgtgggacg aggacagcgg ctaccggtcg
     4201 ttcacggcga ccaagcgggt gacgatccga aacctcgaac tctagcgagg atgtcatgat
     4261 gaaagcgaag ttacagacga tacgagggag cgccctgctc attgctctcc tgctgacagg
     4321 gatgctctcg ctgatcgcca tcatggcgat cgagagctcc aacacggata tcgaactgtc
     4381 gtacagccag tcccacctcg acgccgcgtt ctacaacgcg gaggcggggg cgaaacgggc
     4441 tttcgtggcc ctcaacgata acgacgactg gcggtccggg tacgccgatt tcggcttcgg
     4501 gcaggggacg ttctcggtga acctgtttga ctccctgacc acgcccagcc ttgccgacac
     4561 cgtggtcatt cgctcgcgcg gcgacgtgcg ggaggcacag gccgaggtcg agatgtggac
     4621 tgtgccggtg tacatcaacc cgttcgtctg ggcgatgttc gccgattccg caatcactat
     4681 ggatcagacg acctgcaccg attcctacaa ctccgactcg ggcgcgtacg cggcgaccgt
     4741 gctgcaggac gaggggagca tcggcaccaa cgggcgcatt tcgacttcca agacggtcac
     4801 gatcgggggg gatgccaaga ccgcggtcgg cggcagcatt accctcggtg ccgggagcca
     4861 ggtgctgggc gacacctcga cgacgcagga ttcggtcaac atcgacatca tcccgcagtc
     4921 ggaatacgac tgggcgcgca ccaacagcct ggccccgctg ggtctctccg ggaccggctg
     4981 gagctacaac aacggcaccc gcaaactgac catggggtcg tactccagcg tcgttctcca
     5041 gagcggcgtc tactacttct cggagatcgt gatggggcaa gggtcgacca tcagccttgc
     5101 gccgggagcc caggtcagaa tctacatgag cggcaacatc gacttcaacc aaaacgggcg
     5161 cgtgaacgcg agcggcgccc cgcagaacct ccagatttac tcgcagggca acctcttcca
     5221 gctcgaccag gggaacatct tctgcggtac gtttttcggc cccaacgcgc acatccagta
     5281 cgaccagacg acgcaggttt acggcgccct ggtgggcaac agcatcaagc tcgataaggg
     5341 cgcctgtttc cactacgacc gcaacctcgc caagatccgc cacggcacga ccggcgagat
     5401 gatccaggtg gcgtggaagg agctctgacg gtgccttccg tccgccggac cgagacacag
     5461 aacacggtgg gtcggggcgg ccatgacagc ggccgccccg ccttcacctt tcaatctggg
     5521 gagtacgtac cgatggacaa acctttcacc actgaacggg ggagcgccct gctgatcgcc
     5581 ctgctgctga cagggatgct cacactggtg gccttcatcg ccatggacaa ctccaatacc
     5641 gatatagccc tgtcctacaa cgtgatgaac agcgaccgcg ctttctatgc cgccgaggcc
     5701 ggggtcaagc ggtcgctcgt cgcgctcaac gcggatccct cctggaacga aggcttcgcc
     5761 gacgtcccga tcggcgccgg cagtttcagc gtggcggtcg aggacagctc ggtcactccg
     5821 gccctggccg acaccattat cgtgacttcg accggcctgg tccgcgatgc ccaatcgacg
     5881 atcgagatga cgcttgtgcc ggtccggttc aatccgttcc tcaaagccct tttcggcgac
     5941 aactcggtgg acatcaagaa cagcatggaa tccgattcgt acaactccga ttccggcgcc
     6001 tacgccggca cgcggacgtg gcacgacggg gatgtcggct cgaacggaac gatcacgatc
     6061 gcgaacgggg cgattgtcgg cggcggcgtg gcgacctcgc agcccggcgg gctgagcatc
     6121 aacgccggcg cgaccgtcta cgacgacacg accagcacgg ccccgcggca ggaggtgccc
     6181 gacgtgccgc agacggaatt tgactgggcc gccgcccaca accagaatct cagcggtatt
     6241 tccgggagct tcacctacaa cccctcgacg aaagccttcg agacgtccgg cagtgtgacc
     6301 ctgagcagcg gcgtgtacta tttcagcagt atcatcctga aaaacagcgc ctcgatggag
     6361 gtcgcaccgg gggcgacagt catgatctac gtgacggggg acatcgagat gaaaaactcg
     6421 gcggcgatga acactgccgg tgctcccaaa gacctgcaga tctactcgca gggggacatc
     6481 gtgctcaaga acagcggcga catccatgcc gtgttctatt ccgcgggcgg cgaggccgac
     6541 cttcgtaatt cgggccagtt cttcggggcg gtggtcgcgc gcgacatcgt cgtccacaac
     6601 agcgccaagt tccactacga ccgctcgctg ggcgaaatcg agaagaattg ggacgcccac
     6661 atgaaagtcg tcgcctggcg cgaactctag cgcccgcggc ccaggtccgc gcgaactaca
     6721 gcggctgccg ttcgatggcg acggcagccg ctttctttga tcgtccggcc cgcctccggg
     6781 ctactcggcg ccggacatgc tcttgaccgc gccgggctcc gccagccgcc ggttcttcac
     6841 gtaggtgtcc agccattgcg tcatctccca cagcacgtgg aggaccgatt cccgcgcctg
     6901 gtagccgtgg ctttcgtgcg ggagcatcac gaggcggact gtcgccccct gccctttcag
     6961 agcggcgtag aaccgctcgc tctgcatggg gtacgtgccg gggttgttgt cggcctcgcc
     7021 gtgaatcagt agcagcggtt cgttgatccg gtcggcttgc atgaaggggg acatcgtgaa
     7081 atatgtctgc ggggcctccc agaaggtgcg ctcctccgac tggaacccga agggggtgag
     7141 ggtgcggttg taggcgccgc tgcgggcgat cccggcggcg aacaggtcgg agtgggcgag
     7201 caggttggcg gtcatgaacg cgccgtagga atgcccgccg acagcgatgc ggtcgcggtc
     7261 ggccacgccc cggcggacca tctcgtcgac cgccgcccgg gcgctcgcca ccaactgctc
     7321 cacgtacgtg tcgttcggct ccgcatctcc ctccccgaca atcggcatcg agggatcgtc
     7381 gagaacggcg tatccgagcg ccagccagag caggggagac gaccaggagg cgcggacaaa
     7441 gcggtagggg gagtcggtca cctggcccgc ggcgcccgcc gacttgaact cctccgggta
     7501 ggcccacatg agcatcggca gcgggccgtc ccgcttcggg tcataccgcg gcggcagata
     7561 gagcttgccg ctgagctgca cgccgtcagc ccgcgtatag gtgaccagct ccttcttgac
     7621 gtctctcaac tgcggggtcg ggtgggggaa gaacgtgatc tgcatgagat cgtcgtggcg
     7681 aagatcgcgg cggaaatagt tcggcggctg gtcaaccgat tcccggcggg tgagcaccag
     7741 gcggcggtcg atatcgagga aatccaccgg ctcttcatag tacggggccg cggaacgaaa
     7801 cagacgggtg gtctccttcg tgtccacatt gaaggcatcg aggaagggct ggttgccctc
     7861 cggcgaggcg ccggccccgg tgcgaaagat caccgtcccg gtgtcggccg tcgccaggac
     7921 atacgtgccg cgcccgctcc ggtccagcat cggtgcgccc gggtcctggt aacggtcctc
     7981 ccacgaccgg tcgacgatca gctcggcgcg ggcctccggc cggccgggac ggaggcgcca
     8041 ggtccggacg gtgcgggtcg gccaccaggt ctcgttcacc aacgccaagc tgtcattgcc
     8101 ccactggaca ccggagtaac ggaaacccag cgtcaacagc gccgccggct cgccggtgaa
     8161 cggggccgga agcagaaaga ggcggtcgcg ctcgtcggtg acgacggcgg catccccccc
     8221 gtcgagcgcc tccacccaaa agagcgccgc gtcggcatcc gcgcgccagt cgacgtcgcg
     8281 tgggccggtc ggggccgagc cgaacgtgat cggcacccgg tcggccagcg ggaggtcggc
     8341 gacccggtag acggtcgcgc cggcgcggtc ccacacctcg atcgtgcgcg ggaaacgctc
     8401 ggcgggcacc gtgtacgagt aggggcgatg aatcgtctcc gccagaatgt agcggccgtc
     8461 gggcgaagga gacgtgttta cgaggactcc ggtccgaccg accggcgtca ccgcgccatc
     8521 gacggtcacg agcgccgtct gggcggcccc gtagtagtcg aacaattgct cgtcgcaggg
     8581 gttggccaga aggtcctggt aagtgcgggc gggagctttc gccccgagat tctcctggac
     8641 gttcgggccc tcaggaacgc gcggtttgac gggcggctct ccccgatcgt cggggaggaa
     8701 ccgaacaagc agcgtccggc tgtccgagaa ccatgtgaac gggctgccga acacggcgtt
     8761 caggggccgc tccgaaagcc gccgggcggt gcgtgcagcc aggtcgagca gccacaactc
     8821 aatccgatcc tcgatcgtac aggcaaaagc gacccgtgac ccgtccggag accactctat
     8881 gtcggtgatg cggacgggag agggcagacc cgtgatggga tactcggtcc gatcatcgag
     8941 cgtccgcagc ttcatgctgc tgtaataccc gccgcggctg gggccgttgg tgcgggggtt
     9001 gatgcgctcg ccggcaagcc ggagttccgg ttgggccacc tcctcgagag gcgggagggc
     9061 ggggtactcc aggaggagaa ggttctgtct gtcggggccg agcgacagcc gcggcgtccg
     9121 gggtgcgtcg acgatgtcga caatcgcctg cggcggcgtc tggtaggtga tgttctcctg
     9181 ggccgccggc cccgtcgccg ggccgagcag ggccagaaga aggaagagcg cgcaagcggt
     9241 tcgcagcggg cgagacatca tggcgagact cctgtagaaa agagcacaca tgttgctggt
     9301 cacgccggga gtataacacg gccggcggcc gggcacaaga ccgtcggaat tgtcgttgcg
     9361 tcttgaggcc gcgcccggta gtttggccag cggatgatgc gaactgcgtg ctacatgagc
     9421 cgcgccgggt gcgcgctggc cgcgcttagc gtgggcgcgg ccctctccgc cggctgctcg
     9481 aaatcgaccg atgccgaacc gccgccggcc cccgactcca cgcgcactta cacgtacacg
     9541 gtgatcgaca gcttccccca cgactcgacc gcgtacaccc agggcctcgc tttcggtccc
     9601 gagggttacc tgtacgaggg gaccggtttg tacggcggct cgtcgctgcg ccgggtcgaa
     9661 ctgacgaccg gccgggtcga tacgatgatc ggcctcgccg cgctctattt cggcgagggg
     9721 attgccctgc tggggaatcg catcgtccaa ctcacctgga gagaaaactc ggcgttcgtg
     9781 tggaaccgcg catcgttcgc gcgggaaggt cagttcagct atgcgggcga gggctggggg
     9841 ctgaccttcg acggcagccg gtttattatg agcaacggcg gctcggctct ccagtttcgt
     9901 gacacggcca cgttcgagat ccggcggagc gcccaggttc gcgacgacga cgggccggtt
     9961 gaccgcctca acgaactgga gtatatcgac ggcgaggtct gggccaacat ctggcttacc
    10021 gattggatcg cccggattga cccggacgac ggccgggtga cgggctacgt cgacctgacc
    10081 gggctgctct cgcgaactgc ccacccgacg gcggaggtcc tcaacggcat tgcctgggat
    10141 tcggccgggg cgcggctctt cgtcaccggc aaatactggc cctggctcta ccagatcgaa
    10201 gtgcgggagg cgccgtgacg gccgggcccg gcaggccgct gcgcggcgcg gtcatcgccg
    10261 ccttgctggc ggtgctgccg gccgcctgcg gatcgtcgca gaagcagccg gcgccgccgg
    10321 cgaagatcgc cgcctaccgg gtgaaagtga tccgcacgta tccgcacgac agtctcgctt
    10381 tcacgcaggg cctggcctac gaaggaggct acttgtacga ggggaccggg aaacccggcg
    10441 cgtcgagcgt gcgccgggtc cggctcgaga ccggtgcggt gctgcagcgc cgcgatctgc
    10501 cggcgcccca tttcggcgag ggaatcgctc tggtcggaga ccgcctggtt cagctcacct
    10561 ggacctcccg caccggattc gtgtacgacc gggcgacgct cgcgcctctg tccgagttca
    10621 ggtatctcac cgaaggctgg gggctcacgt acgacgggat ccgtctgatc atgagcgacg
    10681 gcagcgcgac gctgcagctt ctggatccgc agacgtacga gtattgcagt ttcctggccg
    10741 tgcgggccga cggcgaaccg gtcgagcggc tcaacgagct ggagtatgtc gacggcgaga
    10801 ttttcgccaa cgtgtggccc ggcgaccgca tcgcccgtat cagcgccgcc accggacagg
    10861 tcaccggctg gatcgacgcc tccgggctgc tcgcgcccga ggactacggc gacagcaccg
    10921 atgtcctcaa cggtatcgcc tgggatgccg agcaccgtcg tctgttcctc accggcaagt
    10981 gctggcccaa gctgttcgaa gtagaactgg aacccgtgga atgactgcaa ggagtgtgag
    11041 ctgtgcgcgc cggtaagtcg atcgcgacgc tgcggctgga actgatcccg gccacgccct
    11101 cgttgctggc ggccgatgtc gcccgttcgg cccgattggc ggaggaactg ggggcggtcg
    11161 tgccgcccga ctggccgcct tccgaactgg ccgaggtgct cccgcgcctg ctgtcggctc
    11221 tggagcgccg tccggacctg cgcggctggt gggcctacta ctgggtggct tcaggcgagg
    11281 gacgcggacc gcgcaccctg atcggcagcg gcggattcca cggccggccc gatgccgaag
    11341 ggtgcatcga aatcggctac ggcgtgttgc cggacttcca gggccgcgga tacgcgactg
    11401 aggcggtggc tgccctggtc gcctgggcgc tgcgccatcg cacggttacg atggtcttcg
    11461 ccaacaccgg agcggacaac cagccgtcca tccgtctcct ggagcggctc ggattcctcc
    11521 gcgcaggcat cggggccgag ccctggacag tccgtttcga ggttgaccgg cggcgctttc
    11581 gccgccccat gccccggtag cctcctgcta atccggtttt ccgccttgcg ccgaaaaatc
    11641 ccgccggctc atccccgccg cgcgcacgaa tcccagacgc tcataagacg gctggactga
    11701 cggatcgcac atcaggtcga tcatatacag ccccgacagc cgcttaagca gtcgccggac
    11761 caactcgctt ccgatgccct gtccccggta ctcggggaga acctccaaaa gcggaatgta
    11821 ggcggagagg acgcggtcgg tgagagcgtt gatgaagccg acgacgcggc cggcggatgg
    11881 atcgatcgcg agcacgacac agtcgctccg ccggaggatg cgccggtgca tctccggcga
    11941 cggaggattc ggccagccgg cgaaaaagcc ggtcagacgg tcgggcgcga gaccggccgc
    12001 cgaatccaca tactcgatca ccatggatca cctccccttg gcaaagctta gaatgatccc
    12061 caaaacgaac gaccagagcg ccgccaggcc gatctggtac tcggtcggca acgaaaaggt
    12121 cagcgtgtga gccgggatcc agaaccacag gagcgtccac cacgcggtgg ttagcccggt
    12181 catgtcccag cggcggagga tgagattgtc ctcgagccgg tggaagaaca tcatctgcgg
    12241 cccgaaaagg aggttggtga gcacggagac ggcgaacgcc cagccgagcc cggcgccgca
    12301 cgccgcgggc agaaaatgat ggtccagcag ggccgccgta aatcccttca tccccgtgaa
    12361 cccgtacttg atgacaatgc cgagcactgc ccaggccggg actttggcga gcgtttccca
    12421 ccacgttccg gggagggcgg ggcggcctct ccggatgctg accgagacga tttcaccaag
    12481 cgtgcccagc agggcaaact gcagggcggc cgagagcagc gggcgggccg tgacccaggc
    12541 gacgtacgaa tccatcatcg ctccaaaggc ggtaggttga tgtcgacgac agcatatcgg
    12601 ccgcaggcgg tgagttcaag aaaaaacagg agagattaaa cagacaccgg gcgactgccg
    12661 tcattgattg acagcacagg catggcgcgc tattgtcagg catcaaattc aatcgaggag
    12721 gaacgaggat gaccaggtct ggtattgtga cagcgtgggc gctggccaca gcctttctct
    12781 ggactgcagg ccaggcccga gatcaaaaac cggacgccgg tgaggggcgg cagaaccggg
    12841 cgctgtatgt tgcaccgtac gccgcagaca ctgtgattga tccgctgatc aactggccga
    12901 cgtactatgg ccagcagaac acgggactgg tgcgcgcgac attcggggat aatggctcct
    12961 ttgggtggtc cttcctgcgg cgtacgatgg ggattcccgg ttggcccgag gcaggtttcg
    13021 aagtaccggt gagcagcggg gtcgattacc tcttcggagg cgccgtctgg atcggcgggg
    13081 tggtggggag cgacacgctg gtgtccacag gatctgaagg ttgggttaac gtgcgggagt
    13141 tcttccccga tgatctcgcg ggcgcagtcg atgagctgtt cggtccggcg gactttgcga
    13201 tgcgggctga attcaccgat accagcgtcg cccgcgtttc cgtgacggat gagtttgact
    13261 cccgcctgca cctgccgctg gatgtccgga tcaccaaccg cagccatgcc tggcgcacgt
    13321 cgccggccga tgcggctgtc gtctatgacc tggtagtgac caacatcggc gtcgaggcgg
    13381 ttcacgacgg ttatatcggg ttctacttcg actcggatgt ctgcggtgac tgtgacgcca
    13441 actcaggttt cgcggatgat ctctccgggt ctctgcgcga acagggaatt ggctacgtca
    13501 ttgacaacga cggggactca ttgccgggtg agacgccccg ggcgttttgt ttcaagctgc
    13561 tgcactcgtc cgtgccgctg cccgacacga gcttcaactg gtgggcctca agcgcaatcg
    13621 ccgaactgga tttcggtccg cggatgaagg accggccggg cgacccgtac cgggatttcg
    13681 gaaccggcgg gacaggcacc gccgtcgggg atgccaaccg gtactacctg atgcgccatc
    13741 aggagtggga ctacgaccaa gtatggtgtc actccattgg tccggatgat accctctggt
    13801 ggcagccgcc gctgtccctg gcctacgacc tggcggacgg ctttgacgcc cgattcctga
    13861 tgtcgttcgg gccgttcgat ctggcgccgg gccagagcga gcggttcatc atcgccacct
    13921 tcacgaccga ttcgatccac acgctccctg ggaatgccgc caacttgccg ggggacccgg
    13981 cagcctatct ggcgaacctg gacttcacca gcatgatccg caacgccaac gtggccgatt
    14041 cactggccgc gatccttgtt gacccgctgc agccgccggc cggcttgcag gtgagagcga
    14101 ccagcgggaa cgacgttacc atcggttggg atggctcctg cctgcccgag gtgaccggcc
    14161 atgtcgttta cctgtcagtg gttgaccccg cctctcttcc ctatcccggg gttgccccgc
    14221 cctggctgga aatgaccgaa ttggagatcg aggccacgcc cgggccggag cagtccgctt
    14281 ggacgctccc gaaccttgcg actgatcgtt tctatctcgc ctcggtttcc taccggagtg
    14341 cctccgggac cggtgaaccc gcccatccga tcacggtctg ggccggcgag cgcgccgagg
    14401 cgccggaatt tgtcggcagc gagtacgtgt tcgtcgcgcc gggcgacaac atcccgctga
    14461 gctggacgac actttcttcg atggcggttg atcacttcaa tctctaccga ttttctacgc
    14521 cgcaggatgc agagaacgcg taccatgcgt tctacgaccg tgggttcgca gcgtctgcga
    14581 tcgcgccggc ggattcgttc gacgtgaacg ggcggcggta ctactactat gccatgtcgc
    14641 cgtatcaagt acttcccggc gcggcgacat tatttgtgga cgaaccggtt gcggagagca
    14701 cgacctacat cgtcgcggca gtcgatccgg ccggattcga gtcgcacttc tcggagccgg
    14761 tcgaagcggt gcacgtgccg ccgcgcgata aagatatcct cgtgctcacc cacggttcgc
    14821 tcgcgggcgt gcaatttcag agcctcagca gggtggtcga gttctatgac tcactgctga
    14881 ccggctacga ttacgaccta tacgactggg gcgactccaa ccgcatctac atcgaaaccg
    14941 actggcacct gctgatggag tataatctgg tggtcatcga tgacgatctg cgggacggca
    15001 tcttccgccc ggtctgggag gacaccacgc aggcgttcac ccggtacctc ctttctgggg
    15061 ggcgtctggc gtatttcggt tcagggggca gcatggccgg ggtggatgcc attacaggcg
    15121 cgccctcgac gcgaaacctg acccattcgt tggtgagacg gtttttcgga gcggagtccg
    15181 gccgaatcgt cccggccatc tacctggccg ccctctacca ttttccgcca agctatgcgg
    15241 acacgatatt cggattccag cgggccgatc cggcggcgct ggagtacccc gcgctgacct
    15301 atgatctcgc gagcgggcgc atcaaccctg gcgccctgca gctctggcca actaatgcgc
    15361 cgctctcggt ctcgactttc gcgccgaccg ccgccgggga ggtcacccac ttgttcggag
    15421 ccgatgccgc catcaattcg accatggccg gagaacccgt gggtatccgg cgacaggtcg
    15481 gcccggccga aacgtacctg tttggattcc acctgtggta cctggaattg ccgggcgcgc
    15541 gggcgctcat cgacgggatg ttcgaccggg cggtgctggc gcggtttgat ccggataccg
    15601 ttctgatggt atcggcctgg agccttgagc cggagacgct gactgtccat gtgggcaacc
    15661 ttccgtccgg cctgtcggtg tacgacatcg agtcgggatc ggtccggctg tcggagacgg
    15721 tggtccctct gtcaacggag tatacgtccg gtgtgccggg gtttgtgggt gacgtgctcg
    15781 cggtcagggt ctccgccgca gacctgctgt cgctctacgg acaggtctac gggtatttcc
    15841 gaattccgat caaagtctcc ggatccttcg ccggcggcga tccgttcgcc gtgtacgcgg
    15901 ggctcgtgct gctcggcaag ctggtcggag actacgactg gaacggcaag gtgaatatcg
    15961 ccgacatcac catgggggtc gatcgcctct tccgcggtgc ttatccggag ttggcgcccg
    16021 aggacatgga tgtctccggc gactgccggt tcggcgtagc ggatctggcg gtactgattt
    16081 cgtacgtgtt ccgcgacggt ccgccgctca tgcggtgtcc ctgaccgggg cccggtgcgc
    16141 gggagccgga cacaggttcc gggggcgccg aaaggaggtt ctttttcttg ttgtcaagag
    16201 ggagtccgga ggatacattg tcgtcaggaa tggcgccgct gccgaagcgg tggctgtcga
    16261 accggcgctg tgccggtccg aggtttcaac cgtctctcct ttcagggagg tcgcatgctc
    16321 tatatcatcg atccgatcgg caagaagccg agcccgtgcg ggcgcctgag catctacccg
    16381 gtgtgcccgc cggtggtgat ctgcaaagtg tacaagtgat cggcgcttgc tgatcgagtt
    16441 cgtgaggttt gtgtgacacg gcgctccgtc gcgatggcgg gcgccgtggc tctgtttggg
    16501 ggggcgggct tagcggaaat cgcgcgagcg gaacgtgaca tcccccgggg ccgcctcgag
    16561 cggcgcgaac tgcctcccga tcacgttgat cactccgtcc tggcgttcaa gaatcccttg
    16621 cacgatcagc gcctggtggt ggacaatggt gcggcggtgg cgggccagca tgctcggcat
    16681 gacgacgacg ttgatgaacc cggtttcgtc ctccatggtg aggaagagaa aacccttggc
    16741 ggtcaccggg cgctggcgga tgacgaccac gccggcgacc gcgacccggc ttttgtcgcg
    16801 gcgatggcgc aggtcggcgg cggcgaggat tttgcatgcg gccagctggt ctcggatcag
    16861 cgccatcggg tgcggcccgg tggaaagatc catgcccttg aaatcggcaa caatctgctc
    16921 tcctatgtgc atggggcgca atgtcgtatg gcctgtttcc tcaactgcta agtcaagctc
    16981 tcccgcgcgc cgcgaaaggc cgagcacaat ccagagcgcc tgccgccggt gatacccgaa
    17041 acaatcgaac gccccgaccc gcgccaactg cgccagcggc tgcgggccga gacggctgcg
    17101 gcgcacgaaa tctttgagcg aagtgaacgg tccccgggcc tgttcgcttt cgatcgtcgc
    17161 ccgcgcggtt tcgccgatgt gccgcacggt ccgcaggccc agccgcacgg cgtcgccttc
    17221 gaccgtgctg tcgaaccggc tgcgggacac gtcgaccggg aggatcataa cgccgcggcg
    17281 ccgggcctcg tagacgatgc tcgacggcgc gtagaagccc atcggctggg cgttgagcag
    17341 cgagcagtaa aattcctggg ggtaatacac tttcagatac gcggagacgt acacgagcag
    17401 ggcgaaggag gcggcgtgcg actcggcaaa gccgaaatcg gcgaaggcgg ccagttggtc
    17461 gaaaatccgc cgggcggcgt cgtggggaat gccgtgccgc tgcatcccgt cgatgagccg
    17521 ctccgccagc gccgccatcc gctcgcgcga gcgcttgtgc cccatcgcgc ggcgaagttc
    17581 gtccgcctgg ctggccgtga acccggccgc cgctaccgcg accttcatcc cctgctcctg
    17641 aaaaagcggc accccgagag tgcgccggag aatcggctca aggcacgggt gcggataggt
    17701 gaccggctcc tcgccgttgc ggcggcgcag gtaggggtgg accatgtccc cctggatcgg
    17761 gccgggacgg atgagcgcca cttcgatcac gagatcgtaa aaacagcgcg gccggtggcg
    17821 gggaagggcg ttcatctgca cgcgcgactc gacctggaac acgccgagcg tctccgcccg
    17881 gcagaggagg tcgaaaactt tcggatcatc gtaggagagc cgggccgggt cgatcgtgac
    17941 gccgcggtgc tctttcacca gcttgagcgc gaggtcgatc agggtgagca tgcccagccc
    18001 gagcaggtcg atcttgacca gcccggccgc accgacgtcg tccttgtccc actggatcac
    18061 gctccggtcg ggcatcgtcg cattctcgat cggcaccacc tgcgagaggg gcttgcgggt
    18121 gatgatcatg ccgccgacat gaatcccgag gtggcggggg aaacggtgaa tctgacggca
    18181 gaggtccagc agcaggcgga cgcggcggtc gccaagattg agcgccgcct cgcgcagccg
    18241 ctccgcgaca tcctccggcg aggcccagtg gtcgagcagt ttggacaggc gggtgacctc
    18301 gtccggagaa aacccgagcg tcttgccgat atcgcgcacc gccgaccgtc cccgatagct
    18361 gatcacctcg cataccatcg ccgcgtgcgt ccgcccgtac ttggcataca cgtactggat
    18421 gacctcctcg cggcggttgt tggcgatgtc gatgtcgatg tcgggcggct cgcggcgttc
    18481 ctcggaaatg aagcgctcga acagcaggtc gagcttgatc ggatcgaccg ccgtgatccc
    18541 gaggcagtag cagaccgccg agttggcggc cgagccccgc ccttggcaga gaatggcgcg
    18601 ctcggcgcag aaacgggcga tatcccagac gatgaggaag tagccggcca gcttgagccg
    18661 gtcgatgatg cgcagctcgt gtctaagctg gcggacatgc ggctccgcca tctccgggta
    18721 gcgccgccgc gccccggccc aggtcagttc ctccaggtag ctctgctcgg tgtgccccgc
    18781 cggaaccggg aaatcgggca gcgaggtttc gagctgctcg agcgagaacc agcagcgctc
    18841 ggcgatcgcg agggtgtggg ccgccgcctc ggggtagcgg gcgaaccggc gcgccatcac
    18901 cgccggcggc tggaggcacc gctccgcatt cgggtagagc agctcgccgg cctcgtcgag
    18961 cgtggtgtgg tggcggatac aggtaagcac gtcctgcagc cggcggccgt cgggggcggc
    19021 gtagtggaca tcgttggcgg cgaccgcggg aaggccgagc cgccgtccca gccgcaccag
    19081 cgcggcgcag aggtgctcgt gcaccggcag gttgtgatgg cgaagctcga catacacgtg
    19141 gtcgcgcccg tacaggtcgc ggtagtcggc gagcagccgt tccgcctccg agtgctcctc
    19201 ccgcaagaga cacgccgcga ggtcatcgtg gacgagcgcg atgagaccgt cggcgtgccg
    19261 ctccagcaga gcgcgcgaca cccgtgcctc ccccttgggg ctctccaact gggcggtgga
    19321 gagcagctcg gagaggttgg cgtagccggc gccgtctttg accagcagca ccagccggtg
    19381 cccggtgtcc aaaagcgcgg ccgccccgaa aatcggacgg agccccgccc ggcgcgcctg
    19441 ctggtcaaaa cggacaatac cgtacagacc gttggtgtcg gtgatcgcca gcgcgggata
    19501 gccgagttcg accgcccggt cgaccagcgc ctccgggtcg ctggcgccgt cgaggaacga
    19561 aaagttcgag tggcagtgga gttcgatgta gggaacggag gtcatggatc ttctcggtca
    19621 ggccccttgt ggtcctgaca ttcttgccat cgatgaggac gaccccgtca ggaccataaa
    19681 ggtcctgacc tacaagaaaa atctactcac cgaagtctcc ctcaaggtta tccggtttta
    19741 catttcccca accctcgtca taaaatcctc gctcggcgaa ccatgccagg gaggagtgcc
    19801 ggtagtcgaa agggttgcct acgaggccgt gtttgaccgg gttataatgg atatagtcta
    19861 catggcgttg cagatctgcc ggactgcgga tgacatgatc ccagtagcga tgctgccaca
    19921 agcggccctg gcgcacatga tgtcgagccc gatagagtgt ggagaaattc tgtttcatgc
    19981 gatggatgat tacatcgggc tgagtattcc gcggttgcag aatagcatgc cagtgctcgg
    20041 gttgaattac ccacgccagt attcggtatt tcaggaccat gcgggttctc ctgatagcgg
    20101 acagcagcaa atccgcgcag tcgaggagaa tgggctgccg gttgtatgta acgcaggtga
    20161 tgaaatatgt ccggccaggt tcgaagaaac gacgcagttt cgacataggt gatttcttgt
    20221 aggtcaggac ccttgtggtc ctgacattcc tcctatcctc gataattcac ccgtcaggac
    20281 cataaaggcc ctgacctgcc atttctcccc atcaatcaaa caccccctgc acaaaccacc
    20341 gcgcccggct tcggtcgaaa aaaacgaggt actgccggtg ctcctccgtc tgtagctcgt
    20401 agtacaagcg gtcaaaaccg gtctgccacc agtcgcccga cagtttccac ggcccccgcc
    20461 gtccggtgac ccgatgcttc cccgaaccat cgcgcaacca accatccgcc ggatgcacct
    20521 cggccggctg gggcggctgc aacagccgga gtccggcgat ccggcgcagg ctgtacgggg
    20581 gaaggcgatc gaggggtgcg gcgccggcgg acaacgaaga ggcggagggg cgtttggccg
    20641 gcgacggcgt cacgggggag aggcggaacg cccgctccgg caggtgcgac ggcagcaggc
    20701 gcgccgtgca caggcgattc ccgctcagcg tgcgccggat tgtcgcggcc gtctccgcat
    20761 cgatctcgcc ccgcgcgccc ggctgcgcga acgcggtctg ctccgcggcc gccgcggcgg
    20821 ggtgcggaat gcggagggtg atgtcgcgca ccgcggcggc cggcgacatc cgttcgagcg
    20881 ccaggcgcac ctggcgcagg aagcgggcgg cggcggccga cggttgttcg accgccgcgg
    20941 tgaaaacctg ctcggtgcgg tcatcaaggt gcaggcagac ctggatctcg cgaaccgcgc
    21001 acccggcgca gcctggctgc gcaagcaggt gcttgagcag tgcttcgatg tgggcaagga
    21061 tcgtcgccgc gtcgcgcacg ggagaatcaa gtcccgccgt tgcgcaccaa tccgcggccg
    21121 ggcggtcggg agtgaacggt tccggatcat ccccgcgcgc ccggcgggcc agcactgcac
    21181 cctcggcgcc gaaccgctcc accacctcgt tggccggcag cgccgccgcc tgcccgaccg
    21241 tggcgattcc cagatccgac agcgtctcag cggtgtcgtc cgacagcgcc aggtgttcaa
    21301 cctggagctg ctgcagccag gcactctcgc tcccgctcgg aacgatcacc cagccgccgg
    21361 gcgcggcctg gcgggcggcg gtcaacgcga caaacttgtt ggcggcgaac ccgatcgcca
    21421 ccgggtaacc gaaaggctgc aggaccgcct ggatccggcg ggccagcccc ggttcatcgc
    21481 cgtacaattt ccccaggcgc gccgactcca gaaaaatcac accgccggtc tcgatctcga
    21541 tttccggccc gactccggcg agcgcgccgc cgatctcccg gagaaccgcc gtttcgcggc
    21601 cgcactcgcg caccccgatg accagcccgg ggcagagaga attcgcccgg gcaaccgtca
    21661 tcccggccgc cacgtcgtat tgggcggcgc gcgcgttgca gatagtcaca gtcgccgtct
    21721 ccccctccgc ggtcgtcagc acaagcgggt gcgcccgcac ttcaggatgg agcctgctgt
    21781 acaacacgag cggaaaatcg ggcagatgga tgcatgccag ccgccgctca ttcatcgaaa
    21841 cacacctcca cgctttctcc ctctttgcag atgcggctcc gggcgaccgt gaggcgcacc
    21901 ccgcggccgg cccgcgcgac cgccaggcga agcgacgcca tgctcggcgg aataaccccg
    21961 gagttgtttt cggtcaacag caggaccagc ccccgggaac ggctgacttt cagacgcagc
    22021 cggtgcagca tgagccccgg gagcggttcc ttttgaccga ccaggtcgag cacgatccac
    22081 gaggcgatac gggattgcag gagcagttcg gcgacacgga gagcggtcgg tccattggcc
    22141 ggagcgaccg tcagaagccg ctccagatcc accccgcagg ccgccgccgc cgcagggaaa
    22201 aacgaccggc tcagatcaat atacaccgcc cgcccgctcc ggctcaaaga ggccagcaga
    22261 gtcagcagca atgtcgtttt ccccgaggac ggcccgccgg tgatctcaat caactggccg
    22321 cagggaatcc cctcatgagg aaaaagagca tcgagcgccg gcaacccggt ccggaacgcc
    22381 agatccaggc gctccgaagc cagcccgacc gcctccggcc actttcgttg aatcagccgc
    22441 ttgagcgcgg ccagatcttg cgggatgtca ccaggagcta tcatacggtt catgctacca
    22501 cgagagacac aacagccgcc gggcgccggg gcggcctgac ggcaggttaa tcggctgata
    22561 ctgttcattt gtgcagtatg tatacgaaaa ttccctgaat tgtcaagggg cggccaatga
    22621 atatgtgaca ccggaatgcg ggttctccgg aaggccggga agaaacggtt cgccgggggg
    22681 tatttccagc aagaacacag acgatggttc ccccggatcg gccggattcg ggttaaccca
    22741 gggcaaggag tactcgtatg agcagcaagc atttactggc ggcaatgacg gttgcgttcc
    22801 tgatgccggt tctagcggcg gcctcggccg aagacaattc cacggttatc gactcgcgga
    22861 tcgaccgggt caccgtatac ccggatcggg cgggcgtggt gcggctcagc acactgaacc
    22921 tcccggcggg ggaacactca atcctgtttg aaggcctgcc gatcggaatc gaccccgcgt
    22981 cgtttcgctc aagcgcccgg ggtcccaggg gaacgatggt gttgggattg acccatgaga
    23041 cggttgcgca cctcgatgct ccccaggcca gggccgcgga actggaaagg cagatacaga
    23101 cgctggagcg ggagacgcgc caggcgctgc aggtgcgcct cgaagtgtta aaccggcagc
    23161 gcgacctgct ggcggcgctg gccaaagagc acggggaaga gccggatcgc aacgggggcg
    23221 tgggacgcct cgatatggcc aactgggcgg ccgcctacga gtttttgtcg cgccacctcg
    23281 tgcaggtggg cgactccgtg cgcctggtca cgatcgccct tgaagacgcc gaccggcagc
    23341 tcgccctgct tcgcgaacag ctcgacaaac tgcgcgccga tcaggggcgc gagacccgta
    23401 cggtccgcgt ggatctggcg ctggcgcagg ccggcacggt agagatcgaa ctgcagtaca
    23461 cggtcgcggg cgcagggtgg cggccaatct acgacgcccg cctccgtacc gccgccgaca
    23521 gcgtcgaact cgcctgtttc ggcgaggtga tccagcggac gggcgaggat tgggaaaacg
    23581 tggcgctcac cctctccacc gcgatcccct cgcaggggat ggagccggcc gctttccggc
    23641 cgttcttcct gagcgtgctg agcgacgcct acgaagcgaa gagacagcgt aacacggcgc
    23701 tcgaggcacc cgcgccggcc atgatggaca tggcggcgat ggggaaggcg gagcaggaac
    23761 tcgcggaggc ggtcaatgcc gcggccatca tcaacgccgg aggacttgcg accaccttca
    23821 cagcggcccg gcgcgaggat gtcccgtgtg acggtcgcgg cgtccgggct cccatcgccc
    23881 agtggacctt cggcggcaca ctcgccctca tcagccgccc gcgctttgcc gacggcgcgt
    23941 accgccgcgt gaccatgacc aacacctccg acgctccttt cctgcctgga cgggtggcgg
    24001 ttttcgccgg gtcggatttc ctcggatcga cggcgttcac gaaattcatc gccgcggggg
    24061 agaccttcga cctcccgttc gggcgcgata acgacgtcac ggtcgagcgg aaaatcgtcc
    24121 gccacgaggt cggcgacggt tccacctggc tgggaatcgg cggcaaccgg cggcgggtga
    24181 gcgaaacggt cgcgatcacc atcaagaaca acggccgccg cgcgcggaca atcacgatcg
    24241 aggaaccgct gccggtcagc caggacgacc gaatcgaggt cagcttgaag gatttcagcc
    24301 ggaaaccgga cgatcaggat gaatcaggcc gggcgatctg gacggtgacc gtcaatcccg
    24361 gggcggagga ccggctgacc taccggtacg agattgtcca ccccgccgac gtgatcatca
    24421 gcggaatcta gccttcggcc ctccccaggc gcgctcgcga caaccgccgc cgggactccc
    24481 cccggcggcg gctttcctgt gacagagaaa tgacggaagg ccttgaccca ggtcaaggag
    24541 tcttcgcccg ccgccggcga tcctggtaca gcatgatgga gagagattaa cgacgatgcc
    24601 ggacacgatg gaacagccaa gcgcgagaca gaccgccccg ccgaaacccg cccggcgccc
    24661 ccgctatgtc cggcgcgacc ggagcgacaa catccagaag tggcgcttcg ccgcccaact
    24721 cttctcgacc gcggtcagcc tgtggatcgg cgtgcaattc tacctttgga cgaagaagct
    24781 cgagggcgcg gccttgtggt cgtgggtgcc ccgcccgccg ggcgtcgagg gctggctgcc
    24841 gatcggcgcc atcgtctccc tccgctactg gatcgaaagc cacgtgttca attatgtcca
    24901 ccccgccggt ctcgtcatct tcctgttcat ccttggttcc tcgtggcttt tcaagaaggc
    24961 gttctgttcg tgggtttgtc cggtcgggtt catctcggaa atgctgggcg atttctcgaa
    25021 acgattcctg cgtctgagaa tccgcccctg gaaagtgctc gactggccgc tccggatgat
    25081 caagtacctc ctcctcggtt tcttcgtctg ggcgatcctc atccagatga ccccggcgtc
    25141 gatcgaggcg ttcgtctact cgaactacaa ccaggtcgcc gatattctca tgctccgctt
    25201 cttcaccgac atctcccgct tcgccctcgc cgtgaccggc gccctggttt tgctgtcgct
    25261 ggtcgtgcgc ggcttctggt gccgctacct gtgtccctac ggcgctcttc tcggcctgat
    25321 cggattgatc tcgcccaccc gcatccggcg gaacgagacg acctgtatcg actgcgcccg
    25381 ctgcgccaag gcctgcccgg ccttcatcaa ggttgacaag gtaaaatacg tggtctccga
    25441 tgagtgcgtg ggctgcatgg cctgcgtcga cagctgcccg gtgaaggatg ccctgacagt
    25501 gcacatgttc acgaaaaaac ggcccgtcaa caagcgctac tgggcgcttg ccctggtcgt
    25561 cttgttctgg gtcgggctgg ccgggttcaa gctgttcgga ccctgggaaa acaacatcag
    25621 cgacgcccag taccgcgagc acctgccgcg cctcgagcgg ggagaatacg tccacccgcg
    25681 ttaggccgac acgcttgcgg ggcggggaca atcaccgtat catccctccc atgaatgagt
    25741 cattcccgtc tgtttttgag gtctatgccc acgagtacga cctcatcacc aacgcggccc
    25801 agcgcgagcc gcaccacgcc gccgaagtgg cggcgatgat cgcccggttc gcgccggcct
    25861 cggttctgga tgccggctgc gccaccggtc tcacgaccct cctgtttgcc cggcaggggg
    25921 tggctgcggt tggtcttgac cgctctcccc ggatcctcgc cgtagcccgg cagacccgct
    25981 gtccggaggg actgccgatc tcgtaccgcc ggggtacttt cgaggatctg ccgaaatcgc
    26041 tgcgcaaccg ctttgatctg gtcgtgtgtt tggccaacgc catcgcagga gtcggcaccg
    26101 tcgcccgctt gaacctggcc ctgcggaact tttccgccgc cctccggccc ggcggtcacc
    26161 tcgtgctcca gatgctcaac ttcgcggtgg tcaaggaaaa cacgctcctg ccggtgaagg
    26221 ccacccacaa cgacggcctc gtctacgagc gcttctcgga acggcggggc aagacgctgg
    26281 cgatctacgt cacccgcgcc gacttcagcc ggacgccccc gacgttcgag gtgttccgcc
    26341 acagtttcgg cagtttcacg cccgagcagg tgacgggcag catcgccagg gccgggttta
    26401 cggacctcgc ccggttcggc gatctgctgt tcacccggcg tttctcgcgc cgctcccggg
    26461 acctcgtcat tgccgcccgg aggccggcgt agctttttat tgacgcccgg ctgcggtttg
    26521 ctatcttggc ccgatcatga agaacctctg gtggaccctc gccgcagttc tcctcggcgc
    26581 cggggcggca agcgcccagc gggccgttgc tccccggccg tcgctcgagg ctttccgccc
    26641 ggtcggagaa taccgcctgt ggacattcat ccagaaggac acggtcctcg gtacgatcgc
    26701 ctcgaccgtc gagcgtacgg tcgagatcgg cggcctccgc ggctacgcct tgagggagga
    26761 tttcctcctt gatttcacca agaggagcgg ggagaaccgt ttcgccgccg ccggcgagca
    26821 cttcgtcggc gctgacggcg cttacctcgg cgatgatctc aagctgaatg tgaacggcca
    26881 ggaggggcgg atcaacgtga agcgcaaggg ggacgtgatc cgggggctgg tcgggcaggg
    26941 ccggcagcgc cgcgaagcca cggtcacggt gccgtccgga atgttcgcga tcgacaacta
    27001 catggccgac cagttcgaga tctatttcgc cctgcgcggc ctgacggccg gggagactat
    27061 cgacgattcc atctttgtcc cccgcgacct gctggcggcg ccgctgcgtg ggagagtgac
    27121 gaatttttcg tgggtgcagc tctacaaagg ggtgtttgac tcggtcttca cggtgcagct
    27181 cgaccagccg gtcccggtgg agctgtacat caaccgcgag ctccacctgc ggaagctgaa
    27241 ctaccgcgcc caggacatgc gcgtctacct cgacgttgtc cgggagcagg cggagctggt
    27301 caagccccgg ctcacgctcg gcgcgctcgc gcggatgatt cccaagtatc tggtgttcgc
    27361 gctcatcggc ctggtggggc tggtgctcta cgcggggtcg gcggttcgcc gcctcaccct
    27421 ctggctgggc gcgctgaccg gcgcggtcgg cttcg
//
LOCUS       DAOPOE010000037        27393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2239_length_27393_cov_5.258322, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000037 DAOPOE010000000
VERSION     DAOPOE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_27393_cov_5.258322"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKM94_06650"
     CDS             <1..316
                     /locus_tag="PKM94_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD66357.1"
                     /translation="PGDIAEAREAIDLGFVISVGGVITFGDTKMARVAQAVPLDRIIL
                     ETDAPFLTPAPHRGKTNYPAYVRYVYRRLAELKELPAAHLERTVDGTVQKLFGLVETL
                     EG"
     gene            319..1116
                     /gene="rsmA"
                     /locus_tag="PKM94_06655"
     CDS             319..1116
                     /gene="rsmA"
                     /locus_tag="PKM94_06655"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOD66358.1"
                     /translation="MPGHRPQKRLGQHFLVSAEIVRKLIDLIAASPEDVIVEIGPGRG
                     ALTVPLAASGARVVAVEFDRNLAGPLAASLRGYARTTVLNQDFLAFDPERAGLERFVL
                     VGNLPYNLTSPVVEWAVQRRAHILRAVFMVQREVAARLCAAPGGKDWSPLGIFTQLVF
                     EAQRRFDVGPEHFRPRPKVHSAVVELRPRTDAPAAPEGFEPVVRAAFARRRKQLVNNL
                     APALVPDAAAAQRAIAACGLPAQVRAEQLTTEDFFNLTRRLRSGSID"
     gene            1165..1899
                     /locus_tag="PKM94_06660"
     CDS             1165..1899
                     /locus_tag="PKM94_06660"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD66359.1"
                     /translation="MSSLTVNVNLIAMLRELRRLEEPDPAQAAVLAELNGAEGIAVQV
                     RRDRRFVRERDLYVLQAVAKTKLVVELPPADDLIGRMMEIKPHTVVFAADQPDLEAQA
                     APIDFSAPQVDFRDLTERLAAVGVQVAYLVEPEADQIKGASKARANAVLIDCGGYTRA
                     RRREEAQRELDRIDEAGRLAAKAELTVMLGRGITYRNVAPLAELGYVDDFVIGQAIAG
                     RGMLVGFGQAVREMAALVRRQEPTAN"
     gene            1912..2892
                     /locus_tag="PKM94_06665"
     CDS             1912..2892
                     /locus_tag="PKM94_06665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD66360.1"
                     /translation="MAETRELTITVPETVAAERVDRFLAARAELNLTRNRIQRLIAKG
                     LITVNGAAVAKRHEITAGDTIALVIPPPPPRAVIPEDLPLTIVYEDEFLAVVNKPAGM
                     IAHPAAGRRAGTLVNALVHRYGTLAAGSAEDRPGIVHRLDRDTTGLLVVARNDDVYLA
                     LQKKLQAREIHRHYLALVCGHVREEEGTIDAPIGRSTAGRRKMQVNGIRARQAVTHYR
                     LKDRFRSYDLLEVRLETGRTHQIRVHLAHLGHPVFGDPDYGGRESWHRGIHGPERPLA
                     ARLLAVMNRQALHAFRLAFTHPATGEALRFEAPPPEDFAELLRLLDAEGR"
     gene            2939..3772
                     /locus_tag="PKM94_06670"
     CDS             2939..3772
                     /locus_tag="PKM94_06670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66361.1"
                     /translation="MDDTKRITEANRRAWNEATPLHQRHRTVDLRAKFAEPGYSCLDP
                     IETAKLLQIGLAGQRVAQLCCNNGREILSLVNLGAAEGVGFDISDAAIAEAEELARIA
                     GANCRFVRTDVYDIGREWDGRFDLVYITIGALSWLPDLGRFFGVAARLLAPGAALAVY
                     EQHPFLYLFPTPGDPEYDSENELTAVFSYFRTAPWEETQGIDYIGGTTYASPTSYSHT
                     QKISDILNPILRSGLMLREFHEYPHDISLVWRHLEKYHVVPLCYLLVAHKPKDGAPAG
                     P"
     gene            3886..4314
                     /locus_tag="PKM94_06675"
     CDS             3886..4314
                     /locus_tag="PKM94_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015117153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD66362.1"
                     /translation="MSRVKNGDTVTVHYTGRLADGTVFDSSREREPLVVNVGSGDVIA
                     GFEKGLVGMAVGETRTLSLEPEEAYGDHRDDLVVQVETGQFPDHITPRIGEQLQVRQP
                     DGSVVEVEITNIEDGLVTLDANHPLAGERLLFDVEIMGIG"
     gene            complement(4376..5212)
                     /locus_tag="PKM94_06680"
     CDS             complement(4376..5212)
                     /locus_tag="PKM94_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD66363.1"
                     /translation="MSGILFLRTGDLERTAAFYIARLDCRRWLDQPGCVILRHDNFLF
                     GLCARERPQTDVLLTFFYDTPAEVDRLYARHRDTADGPPRLNETYRIYQFFARDPEGR
                     GLEFQHFADPVAQFRSGDDLLATRRSVREFAPDPVPDDVIERIIESCRFAPSAWNRQP
                     QCYRIIRDPETLRYLAASRQEATAPIGRAPAAVAVSVDTTVSPYPAVDGAIAAYHFLL
                     AAWFYGLGTCWMGNMDAPEIKARLGLPPGHFLATVTPLGYPKERAAPPPARKPFAAFL
                     RG"
     gene            complement(5251..5580)
                     /locus_tag="PKM94_06685"
     CDS             complement(5251..5580)
                     /locus_tag="PKM94_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66364.1"
                     /translation="MTTYRSDDLEGWEFKIVRSLTGAFSKPEKVEQLRRDEAQAGWEM
                     IEKFDNNRIRFKRRTDRRANDHLLPFDPYRTNYGIGEGRMAAVVITSMVVVIGLVIVL
                     ALLFGDR"
     gene            5871..6755
                     /locus_tag="PKM94_06690"
     CDS             5871..6755
                     /locus_tag="PKM94_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOD66365.1"
                     /translation="MAPLVTIVEWWRRHPRLVQGYTSRRLGNLSYRTAPAAEAAERRV
                     RLAAELGIDPGRMFSIPLGHTNRVVVLRDDSFLARRDRRGYVEPEADEILAWPVLTPE
                     AYAAMPHDREYADGVVFAAANVYALVITADCAAVGFYDPVRQAAGNAHVGLLGAVNEL
                     PKGMVAALVESFGSRPADIEAVIFPCIRPCHYATANSQTWLRIRERVHAAFGEDNPLF
                     ASGYFDLPGMVRSQLRASGLLEEHIFDVGLCTVCNADTLFSHVAAGSAEAQAREGRFG
                     AVMGFRPEAPGGPTGGGR"
     gene            6796..7218
                     /locus_tag="PKM94_06695"
     CDS             6796..7218
                     /locus_tag="PKM94_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOD66366.1"
                     /translation="MSSMNHDDPPPGLPERLLRYHLPVLLYAGGIIALSSIRDFRPPD
                     LPIPSLDKVVHLLEYAVFAYLVFRSFYHMGRSPRLGRALLWSALFVTFFALADEMYQR
                     YVPGRHSDWRDFVVDVLGALLVLGILGIYRWRRRRRSY"
     gene            7717..12231
                     /locus_tag="PKM94_06700"
     CDS             7717..12231
                     /locus_tag="PKM94_06700"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOD66367.1"
                     /translation="MKLRLLMALVVALGLSAGSVLAQAPPPQDPFGTMDTVALVAVGD
                     PASGHFQVELWVYSDEDIGGATTGFVWDNAKVQMDSAKASALTAGAFTLGTFFYEQSN
                     ITLTNSNRRFLFGGLAFTSALPGDVSTRRLWATYYFNTTGWTGSDNVTFETQVWGIGT
                     DYSYVEFNTNEEFYPVWMGALTIPPPSLNDPPVLAAIGAKSVNEGALLSFGVSATDPN
                     GDAITLTASPLPTGANFTDNGNGTGTFSWTPNFTQANVYNIKFKAVDSKNASDSEIVA
                     ITVNNVNQAPVLNAIGPKSVDEGALLTFPVSASDPDGTIPALSATNLPAGASFTDNGN
                     GTGTFSWTPNFTQAGVFNVTFAASDGVLTDDEIVAITVSNVNQAPVLAAIGPQSTPVE
                     VLLSFGVSATDPDGTIPTLSATNLPTGASFTDNGDGTGTFDWTPAIGQEGVHNVTFTA
                     SDGVLTDDEIVAITVTGGANEAPVLNAIGGKTIDENALLQFTVSASDPDGGFPELTAE
                     DLPTGATFVDSLNGVGLFTWTPDFTQAGVYNVRFIASDGLLADSELVEITVNNVNRPP
                     VLITMAEDTTIDECGSASFWFDATDPDGGVLTFSADGLVANMAFDQLDSGALFTFNPD
                     TTQAGSYTVTVRVQDALLATDSVIFTITVEECTTPPPCVDLILSDTVLFIHDTITFAD
                     GPYGFRVVTVGSTGPGAGCFIVLDTTAWLSATPPETCTPANVTISYDATGLPAGDYYG
                     IVMFQGDATVCEPRTYYVMVALQLVDTTTVPEGADTLFVGIVPAAPGAEVVVPMEFTN
                     SCDLFSAQATLEWNPAYLTLDSVGFEGSRLDDFPVKAVDIFAGHAVISADFGGSAPMV
                     GPGFGGFANLYLTVAPGAPVGFHEISIVTPTEDTYFGINCGGPDLFIPPVPENGGVAI
                     DTSTAFTCGYVVDTAGDPIAGATVEMWSGFPGMGPEASTTSGADGYFEFTTELVYGQF
                     DLWAYKEGYYPGLIENVNWGETGIMIVLTPYAPVYPTLYWVDFYCDANTYFGSPLPIG
                     SVIDAYDPDGVRCGSWFVKEAGKYGFMPVYGEEEWNEGDQGAEPGDEIRFFINGVEAE
                     ATGNTLWANAPTVPQMVCLNLDVCRQVCDLTAGWNLVSWSIDTESDDIEAALASIAPH
                     IQLVLGFEQGGLTYDPTLPQFSTLWYVDHLSGYWIKVDADVEMVIEGNCANVSTPITV
                     TPGWNLVSYLPHVAMPTPDALTSIYDDLMVALGFDGGALIYVPSDSGFNTLDEMAPCF
                     GYWVKVDAPGELIYPSDGPVVFAQQRQPLGHPLPKAAEGVTATTQWMNLYAQKLTVDG
                     AVVPTGARVEAFTLDGHRIGSSQVRADGLFGFMPVYADDPNTSAVDGLRTGEPFTLTV
                     NGAATAETFVFTGHGTNLEVSGLTAKAGEEPTMPGTYALAQNYPNPFNPTTTIEFSLP
                     KETRAKVEVFNVLGELVATPFDGTAAAGTTSVTWDGRSRTGSPVASGVYFYRLSADNY
                     TETKKMMLMK"
     gene            12315..13343
                     /locus_tag="PKM94_06705"
     CDS             12315..13343
                     /locus_tag="PKM94_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD66368.1"
                     /translation="MVKRRFVRLLIVAAAAALPTSPLWAQGIIPTPYSDQFKGTVMIN
                     GQPAPVGTVIDAFDPDGVNNGRDTTRFGGLFGYFAAYGDDVSNTPALDEGARENDPIT
                     FRILGRPAAIDSGDATWNNMDTSTVHLSVSGVTIGLLLVDAPLTGVGSPRDTVRFEVG
                     IRNTGNIRDFYDIIVEQSKVPAWGVIYPAELVYNGPDSTAYLWFDVVVADFPGEPPLN
                     HLTYTVRSRIDTTATASGTVDLDVDVVGTEDRDELLPGAFALAQNYPNPFNPTTTIAF
                     TLPRASTVRLEVYNTLGRLVEERDLGTYPVGDHAVAFDGTSLASGVYLYRVATEYGSA
                     SRKMVLMK"
     gene            13494..14411
                     /locus_tag="PKM94_06710"
     CDS             13494..14411
                     /locus_tag="PKM94_06710"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOD66369.1"
                     /translation="MALVIQAVGKTDVGLVRKGNEDNLYLDDRNHLYVVCDGMGGHQA
                     GEVASMSAVTTLETVHNQLRREILGDERLSPGRALPDETNLLLRAIRLANREIYGKAA
                     ANSSMAGMGTTIVAIAFEHDLAGIAHVGDSRAYRLEARRLVPLTADHSWVSEMQAAHN
                     LSAQEAESLVGRNVITRALGVREAVEVDCRLVRVKPGDILLMCSDGLCGFADDDEIFE
                     AAHRLRDDLKKMVDALVQFANDRGGNDNVTVVAMRVMEAKATDIPELPVFTLTAESRE
                     VLAAEDEWLPKIREAAEALHAGGKRQKKK"
     gene            14610..14683
                     /locus_tag="PKM94_06715"
     tRNA            14610..14683
                     /locus_tag="PKM94_06715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14644..14646,aa:Arg,seq:acg)
     gene            complement(14747..15736)
                     /locus_tag="PKM94_06720"
     CDS             complement(14747..15736)
                     /locus_tag="PKM94_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOD66370.1"
                     /translation="MKATWLPAGVLAATALVFWACSGDDDSTNPDQPTIPTVTTSAVS
                     AVGSTTATCGGTVTADGGAEVTARGVCWRTSADPTIADDTTSDGIGVGSFTSSLTGLS
                     GGESYHMRAYATNRAGTGYGEDVPFATLGTVADYDGNTYQTVKIFNRWWMAENLKVTH
                     YRNGDPVSDVTDPTAWNALASGACCEYANDPANVAIYGRLYNWFAVGDARGLAPAGWH
                     VATDSEWQELVDSLGGGAVAGGKLKAAGTAYWADPNAGATNESGFSGLPGGYRGGMGD
                     FNGIHYGAGFWTATPHQYFSDQAWNRNLGYNRPEMVHDYSGKKSGNAVRCVKD"
     gene            complement(15858..16712)
                     /locus_tag="PKM94_06725"
     CDS             complement(15858..16712)
                     /locus_tag="PKM94_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOD66371.1"
                     /translation="MRWQGRRTSDNVEDRRGIGGRGLAVGGGLGGILAVVLFLVLGGN
                     PDEVMQSLQQTGVEVPAGSTAELSDEERQEGQFVGVILAETEDVWNRLFQQAGQSYRE
                     PKLVLFSDQISSACGYAGASAGPFYCPADERVYIDLSFFKEMQAQLGAHGDFALAYVI
                     AHEVGHHVQNLLGISDQVMGQRERMSEAEFNRLTVRMELQADFLAGVWAHFAERTANL
                     LESGDIEEGINAAAAVGDDRIMRQTQGYVVPDAFTHGTSEQRVRWFRRGYESGDITQG
                     DTFSSQEL"
     gene            complement(16873..17607)
                     /locus_tag="PKM94_06730"
     CDS             complement(16873..17607)
                     /locus_tag="PKM94_06730"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOD66372.1"
                     /translation="MKVIGLIGGTGWVSTCAYYRLINELTNERRGGLHAARCLLYSFD
                     YADIDALNRAGDARGVFGLVRAAADSLERAGAECLVLCANTLHQFAEELAGCTRLPLV
                     NVAEAAADEISRRHFSTVGLLGTRMTMEENFYKDVLRRRGIRVRVPDRDDCAFIHTTI
                     LTELLKNVVSDRAKDRFRRIIAGLADGGAEAVVLGCTELPLLVAPEDVEIPLIDTLRL
                     HCRAAVDFALAQTVRTPPEAQSDMPA"
     gene            complement(17625..19640)
                     /locus_tag="PKM94_06735"
     CDS             complement(17625..19640)
                     /locus_tag="PKM94_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD66373.1"
                     /translation="MTGRTFALCAAVVLLAAVRLPAAEIHDAVAAGDVERVRVLLAAD
                     PSLINARDSAQNLPLHVAALQGRTAMIELLLAEGADINIGDRENTSPLTIAAMRNHLD
                     AVRLLAERGAQAAARDINGETPLISAARRGNLEMVRCLVAHGAPLYETSARGSNCLHI
                     AASIGADSLAAYLLAQEFDINARNGDGRDALYFAAANGFTSIALRLIAQGADVQYRNE
                     QGETRLHTAVFRGDTVLAGALVAAGLAIDARDNWGVTPLTNACWGTVDMVRWLLDHGA
                     DVNARTDSTHTPLSIAVLAGHADLVRLLVGRGADVNYHGRLSGPALQSAAVQGNDEIA
                     GILLQAGADPEFTDADYGRTMLHIAAIRGDSALARVLLDHGVPPAAQDRSGRTPLYYA
                     VHYANPSVAALLRDAGAAPLPAESEADPPDLLAATLGEKEAVVWYLNHSGWAIRTARH
                     FLIFDYALPPCNPDSPCLANGRIDPSEIKDLKVMVFSSHEHRDHYDTSIFAWRNAIPD
                     ISYVLGHRQDGAPAHRYLGPRSDEVIDGMRVRTIDATDAGVGFLVEVDGVVIFHAGDH
                     ANGQVGLHAPYTDEIDYLASLGAPIDLAFLPILGCSLGTPESVREGVVYALQKLSPAV
                     YFPQHAMNSEYRFRAFSDGLKRSGLTVQTGLAENGGDCFRYRGNRIL"
     gene            complement(19927..20787)
                     /locus_tag="PKM94_06740"
     CDS             complement(19927..20787)
                     /locus_tag="PKM94_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66374.1"
                     /translation="MQRVLIVLSALAVFLLLPLGCSDDESCADCPATPAGFVDGSITV
                     NPQAIVNAVIYGYGGIVPSIDSIRLGDSLMTRPTDYNWFQDAPGRRHWQIGFSESAGG
                     SFLYSSGDVARLEIWGPGRQAVCDVVMLDPRDSEAQIISPVPGEDTVAPGESVTIVWQ
                     RIEHAQFYALMLTFFNRANAVTRQSLVTDWTADTSYTVRPVLYPDSLQLISVELSGFT
                     GPNPSVGSNFSGGWLTGRLISFSAPAHTFIWGWNAPDKARAEFPDEAAVSAPRSPAEI
                     IDAIYRQAVR"
     gene            complement(21008..21865)
                     /locus_tag="PKM94_06745"
     CDS             complement(21008..21865)
                     /locus_tag="PKM94_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66375.1"
                     /translation="MQRIVLVLSAVAVSLLPLGCSDDCADCPEHATPAGFVNGTVHVT
                     PQVSLSATINGYGSILPTVDSIRLGDSLLTSSGDYYVAMSAPANPLWRISFYENISGD
                     VMYSPGDVAHIEIWGPGRLAYCDVVVLDPNESAADMISPLPGTETIAPDESAAVIWHR
                     ALDAQYYAVQLRFIDFANGVTRESNVTDWTADTAYTVGPTLYPDSLAALIVEITPFTG
                     PNPAVGSNFAGGWLAGRLISFGGPASTMLHGWNEPDKSIKTLPDDLAASQRLSPAEII
                     DGIYRQAAK"
     gene            complement(22082..22945)
                     /locus_tag="PKM94_06750"
     CDS             complement(22082..22945)
                     /locus_tag="PKM94_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66376.1"
                     /translation="MKRTMIVLSAVVVALLPLGCSDDDCAECPAPVTPVGFIQGSIDL
                     HPGVYFEALIFGDGSILPTLDSARVGDSLLVFNKDFFYSYRSEPVNPYWTIYCTEDTG
                     PDYMYTQGDTAHLEFWSEGRLATCDLPLLDPNLTQLFITDPYPGTDTVAPGEPAIVFW
                     NRVEHAQFYAVQVILANRVGDVFTFSDRFDWTADTSFTVSPSLYPPSLAQFSVRVVPF
                     TGPNPAAGSNVLGAWLSGRIVSVGYGAETAIVGWNPPPITKSALPAGETLFPQRTPAE
                     IVEAVYRQAAE"
     gene            complement(23107..24624)
                     /locus_tag="PKM94_06755"
     CDS             complement(23107..24624)
                     /locus_tag="PKM94_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD66377.1"
                     /translation="MKWKPPRRPKMASTGRACGKRALLAALVLAAACAGCDRTLESAD
                     RHPADLPQIKRRGVLRVLTAFSSTGYFVYRGRTMGFEHELLARLAEHLDLRLEVIPVR
                     DIDSLIILLAAGEGDLVAYNLTVTAERAAQVAFAEPLTSTHQVLVQRKPELWRRMDPR
                     VLESCLIRAPDSLAGRTVHVRHGSAYYERLVELAGEIAGAVDIEIVDGRVPTEELIRR
                     VAAGDITYTVSDQNLAHIIAADYPNLDVGTAVGPPQQIAWAVSKSSPLLLAALNTWLR
                     QFKRTPDYYAVYNKYYRNRKAFRQRPVGAWATVESDRLSPYDGLIRRHAAEIGWDWRL
                     LAAVIYEESQFDPEAESQAGAAGLMQLLPSTAAHFGVIGDDIFDPARSIAAGAAYLDF
                     LERQFSDVPDTVERLKFALAAYNAGAARVQEARRLAERYGEDVRFWTDIVEDYLRLRA
                     DSPAVEDETLRFGYLRSAETADFVRDVFARYEHYCRRMPSATGDQAQTETLSAPR"
     gene            complement(24693..25409)
                     /locus_tag="PKM94_06760"
     CDS             complement(24693..25409)
                     /locus_tag="PKM94_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOD66378.1"
                     /translation="MKTFSANGKDYRVDDKGFLLDPGEWDEGFAEAMAREAGIERGLT
                     EAHWRVIRFIRNTFEQINQCPLIYVACKKNHLGLGDLKKLFPTGWLRGACKLAGVTYR
                     EGYLQHVWLEGDIAHFTNTYERRTYELDEHGFLMNPDDWDENFAVHKAYESGISGNLT
                     ERHWQIIRYLRGAYGRTGTVPTVYETCEANGLELDELEQLFPGGYHRGAVRLAGLHAM
                     ERPARPGGRPDRPRPDSPHK"
     gene            complement(25534..26421)
                     /locus_tag="PKM94_06765"
     CDS             complement(25534..26421)
                     /locus_tag="PKM94_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66379.1"
                     /translation="MVQLVSPLVRKFAATAALVLLSVPTAGAAEFVGDLTLKSARQTM
                     TGRLFVKDSLYRMDFSQDAGKVYVLVDQSAGECRIVNPPARTYRILPLNAPTVVQNDP
                     FQAARIGDSLGVRDYRGADTLEGFPCDRFTIRLGKNPLMNQWIPTRLGFPIRIENLMS
                     PDSAYVAVTNIAIGPVDSALFVIPLDYSLQAEPSPPPRDPRRGAKAGDSVLVRLDTAL
                     AVEVGLIGLDSSGAECVVELLRDGKAIAETAAGPESSRTFRFAAVGEQVRRIYAVAAD
                     GLRVRVTRGEVLIDVSQPR"
     gene            26796..>27393
                     /locus_tag="PKM94_06770"
     CDS             26796..>27393
                     /locus_tag="PKM94_06770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD66380.1"
                     /translation="MRRPLRHPVLPAILAAIILTGLPAAATHAVAGWPVGYDVDLSSG
                     FGDYRFGHFHFGIDIRTGGVTGKEVLAPVDGYVWRLRTSYTGYGKVLYLRGNDGCTYV
                     MAHLQDFNSALDRRLKTAQVSAKRYYQDLYFPEDSVPVKKGELLAYSGQTGSGAPHLH
                     FEKRAGDIPLNPLSHGYRLADKTPPVITRLGLRLTDDHS"
BASE COUNT         4777 a         8665 c         9373 g         4578 t
ORIGIN      
        1 ccctggggac atcgccgagg cgcgcgaggc gatcgatctc gggttcgtca tttccgtcgg
       61 aggggtgatc acgttcggcg atacgaaaat ggcgcgggtg gcccaggcgg ttcctttgga
      121 tcggattatc ctggagaccg atgccccctt cctcaccccc gcgccgcacc gcggcaaaac
      181 caactacccg gcctatgtcc ggtacgtcta ccggcggctg gccgagctca aagaactccc
      241 cgccgcgcac ctcgagcgga cggtcgacgg gacggtgcag aagctgttcg ggctggtcga
      301 gacgctggag gggtgaccat gccggggcat cgcccgcaga agcggctggg gcagcacttc
      361 ctggtgtcgg ccgaaattgt gcggaagctg atcgatctca tcgcggcctc gccggaggac
      421 gtgattgtcg agatcgggcc ggggcggggg gcgctgaccg tgccgctggc ggcgagcggg
      481 gcgcgcgtgg tggccgtgga gttcgaccgc aacctggccg gaccgctggc cgcgtcgctc
      541 cgcggctatg cgcggaccac cgtgctcaac caggatttcc tcgctttcga tccggagcgc
      601 gccgggttgg agcgcttcgt gctcgtcggg aatctcccct acaacctcac ctcgccggtg
      661 gtcgagtggg ccgtgcagcg ccgggcgcac atcctccgcg cggtgttcat ggtgcagcgg
      721 gaagtggccg cgcggctgtg cgcggcgccg gggggaaaag actggtcgcc gctgggcata
      781 ttcacgcagc tggtgttcga ggcgcagcgg cggttcgacg tgggaccgga gcattttcgc
      841 ccgcgaccga aagtgcactc ggcggtcgtg gagcttcgcc cgcggacgga cgcgccggcg
      901 gcgccggagg ggttcgaacc ggtcgtgcgg gcggccttcg cccgacggcg caagcagctg
      961 gtcaacaacc tcgcgcccgc gctcgtaccc gacgccgccg cggcccagcg ggcgatcgcc
     1021 gcctgcggtc tgccggcaca ggtgcgggcc gagcagctca ccaccgagga ctttttcaat
     1081 ttgacgcgcc ggttgcgatc cggtagtatt gactgaccga acgaaagctc taacccaagc
     1141 cttgcggcgc aagcggaggt cggcgtgtcg tcactgacgg taaatgtaaa tctcatcgcc
     1201 atgctgcgcg aactgcggcg cctggaggag cccgatccgg cccaggcggc cgtgctggcg
     1261 gaactcaacg gcgcggaggg gatcgcggtc caggtgcggc gcgaccggcg cttcgtccgg
     1321 gagcgcgacc tctacgtgct ccaggcggtc gccaaaacca agctggtggt cgagctgccg
     1381 ccggccgacg acctcatcgg gcggatgatg gagatcaagc cgcacacggt cgtgttcgcc
     1441 gcggatcagc cggacctcga ggcgcaggcc gcgccgatcg atttcagcgc gccgcaggtc
     1501 gatttccgcg atctgaccga gcgactggcc gcggtcggtg tgcaggtcgc ctacctcgtc
     1561 gagccggagg cggaccagat caagggcgcc tccaaggctc gggccaacgc cgtgctgatc
     1621 gactgcggcg gctacacgcg ggcccgccgg cgcgaggagg cccagcgcga acttgaccgg
     1681 atcgacgagg ccggacggct ggcggccaaa gcggagctca cggtgatgct cggacgggga
     1741 atcacctacc gcaatgtcgc gcccctggcg gaactcggtt atgtcgacga tttcgtgatc
     1801 gggcaggcga tcgccggccg ggggatgctg gtcgggttcg gacaggcggt gcgggaaatg
     1861 gcggctctcg tgcgccgaca ggaaccgacc gcgaactgag cgggcggcgc tatggcggaa
     1921 acccgcgagc tcacgatcac ggttccggag acggtggccg cggagcgggt ggatcgtttc
     1981 ctggcggcgc gggcggagct caatctcacg cgcaaccgga tccagcggct gattgccaag
     2041 gggctgatca cggtcaacgg cgcggccgtc gccaagcggc acgaaatcac ggccggcgac
     2101 acgatcgcgc tggtgattcc gccgccgcct ccgcgcgcgg tgattcccga ggacctcccc
     2161 ctgacgattg tctacgagga cgaattcctc gcggtggtga acaagccggc ggggatgatc
     2221 gcgcacccgg ccgccgggcg ccgtgcgggg acgctggtca acgcgctcgt gcaccgctac
     2281 gggacactcg cggcgggatc ggccgaggac cgcccgggga tcgtgcaccg gctcgaccgc
     2341 gacaccaccg ggctgctggt ggtggcccgc aacgacgacg tctacctcgc cctgcagaaa
     2401 aaactccagg cgcgcgaaat ccaccgccac tacctcgccc tcgtgtgcgg gcacgtgcgc
     2461 gaggaggagg ggacgatcga cgcgccgatc gggcgctcga cggccggccg ccggaagatg
     2521 caggtgaacg gcattcgggc gcggcaggcg gtcacccact accgcctgaa agaccgcttc
     2581 cgctcctacg atctgctcga ggtgcgcctc gagacggggc ggacccacca gatccgcgtg
     2641 cacctggcgc acctcggcca ccccgtgttc ggcgacccgg actacggcgg ccgggagtca
     2701 tggcatcggg ggatccacgg tccggagcgg ccgctggcgg cgcgcctgct ggctgtcatg
     2761 aatcgtcagg cgctccacgc gttccggctg gcgtttacgc accccgccac cggcgaggcg
     2821 ctccggttcg aggcgccgcc gccggaggat ttcgccgaac tgttgcgcct gctcgacgcc
     2881 gaggggcggt gagggagttt tcggtggcgc ccgcaccgcc cgggccctat cttggcgcat
     2941 ggacgacacc aagcgcatca ccgaggccaa tcgcagagcc tggaacgagg cgacgccgct
     3001 ccaccagcgg caccgcacgg tcgatctcag agcgaaattc gccgaaccgg ggtattcctg
     3061 cctcgatccg atcgaaacgg cgaaactgct tcagatcgga ctggcgggac agcgcgtcgc
     3121 gcaattgtgc tgcaacaacg ggcgcgaaat cctctctctg gtaaacctcg gggcggccga
     3181 gggagtcggg ttcgacatct ccgatgccgc gatcgccgag gccgaggaac tggcccgcat
     3241 cgccggcgcc aactgccggt tcgtgcgcac cgacgtgtac gacatcgggc gggagtggga
     3301 cggccggttc gacctcgtct acatcacgat tggggcgctc tcctggctgc ccgacctcgg
     3361 ccgatttttc ggcgttgccg cccgcctgct cgccccgggc gcggccctgg cggtctacga
     3421 acagcacccc ttcctgtatc tcttcccgac gccgggggac cccgagtacg actccgagaa
     3481 cgagctgacc gcggtcttct cgtattttcg gaccgcgccg tgggaggaga cgcaggggat
     3541 cgactacatc ggcggcacca cctatgcgag tccgaccagc tacagccaca cgcagaagat
     3601 ttcggatatt ctcaatccga tcctcagaag cgggctgatg ctgagggagt ttcacgagta
     3661 cccgcacgac atatcgctcg tgtggcgcca tttggagaaa taccacgttg tcccgctgtg
     3721 ctacctgctg gtggcgcaca agccgaagga cggtgctccg gcggggccat gacaatcgcg
     3781 catcgcagtc tgggctgctg agggtttcgg cgccgtcatg cagtgacggg atctcagaag
     3841 tctgggccgg ataatgatca gagcggaacc agacaggagg acgacatgag ccgggtgaaa
     3901 aacggagata ccgtcaccgt ccactacacg gggcggctgg ccgatggaac agtattcgac
     3961 agctcgcgcg aacgcgaacc gctggtggtg aacgtgggct ccggcgatgt gatcgccggt
     4021 ttcgagaagg ggctggtggg gatggctgtg ggggagacgc gcaccctttc gctggagccg
     4081 gaagaggcct acggcgacca ccgcgacgac ctcgtggtcc aggtcgagac cgggcagttc
     4141 cccgaccaca tcacgccgcg aatcggcgag cagctccagg tgcggcagcc ggacggcagc
     4201 gtcgtggaag tcgagatcac gaatattgag gacggtctgg tgacgctcga cgccaaccat
     4261 ccgctcgccg gcgaacgcct cctgttcgac gtcgagatca tggggatcgg gtagcgggcg
     4321 gccggccggc gcatcgcacg ggccaacgtg cgaggcgccg cggggtccgg cgggctcagc
     4381 cgcggagaaa ggcggcgaaa ggcttgcgcg ccggcggcgg cgccgcgcgc tccttcggat
     4441 aacccagcgg cgtgaccgtg gcgaggaagt gccccggcgg gaggccgagg cgggccttga
     4501 tctccggcgc atccatgttt cccatccagc acgtcccgag accgtaaaac cacgccgcga
     4561 gcaggaaatg gtacgcggca atagcgccgt cgacggccgg gtaggggctg acggtggtat
     4621 cgacgctcac cgcgaccgcc gccggcgccc ggccgatcgg ggcggtcgcc tcctgccggc
     4681 tggcggccag atagcgcagc gtctcgggat cgcggataat ccggtaacac tgcggctggc
     4741 ggttccaggc gctcggagca aaacggcaac tctcgatgat gcgttcgatc acgtcgtcgg
     4801 ggacgggatc gggcgcgaat tcgcggacgc tgcggcgggt cgccaacaga tcgtcgccgc
     4861 tgcggaactg ggccacggga tcggcgaaat gctggaattc gagcccccgg ccctccgggt
     4921 cgcgggcgaa gaactggtag atgcggtagg tctcgttgag ccgcggcggc ccatcggcgg
     4981 tatcccggtg gcgggcgtac aggcggtcga cctcggcggg agtgtcgtag aagaaggtga
     5041 ggagaacgtc cgtctgcggg cgctcgcggg cgcacaagcc gaacaggaaa ttgtcgtggc
     5101 ggagtatgac gcaccccggc tgatcgagcc accggcggca gtcgaggcgg gcgatgtaga
     5161 aggccgctgt ccgctccagg tcccccgtgc gcaaaaagag aatgccgctc atggtgtcag
     5221 ggcgcaaccg ctcgcccgag cccgtccctc tcagcggtcg ccgaatagga gcgcgagcac
     5281 gatcacgagg ccgatcacga ccaccatgga tgtgataacg accgccgcca tccgcccctc
     5341 gccgatcccg tagttcgtgc ggtacggatc gaacggcagc agatgatcgt tggcgcggcg
     5401 gtcggtgcgg cgtttgaaac ggatgcggtt gttgtcgaac ttttcgatca tctcccagcc
     5461 cgcctgcgcc tcgtcgcggc ggagctgctc aaccttctcc ggcttgctga aagcgccggt
     5521 cagggagcgg acgattttga actcccagcc ctccaggtca tcggaacgat acgtggtcaa
     5581 gacttcctcc tcctggcgtt cctgttcttg cttcgcctgc gccgcaatca ccgcggcccc
     5641 tccggcactc attctgcacc tccggtttga aacaattacg ccgcgtgcgc cgggtctatt
     5701 caagaaaaaa gattgctggt tgacacgagg ggaccaaatg cgcgagtatc gggggtggtt
     5761 cgggaacagg cgggggcgcc cccgggcggc tgcattcggc gccttcccgg tacgcggccc
     5821 gcttgcaggg aaaaggagag atgcgcccgc ggccggcggg cgacgcggca atggcaccgc
     5881 tggtcacgat agtggaatgg tggcggaggc acccccgcct ggtgcaggga tatacgtccc
     5941 ggcggctggg caacctgtcc taccgcaccg cgccggcggc cgaggcggcc gagcggcggg
     6001 tgcgtctggc ggccgaactg gggatcgatc ccggccggat gttctcgatt cccctcgggc
     6061 acaccaaccg cgtggttgtg ctccgcgatg attcctttct cgcccgccgc gaccgccggg
     6121 gatatgtgga accggaggcc gacgagatcc tggcctggcc ggtgctgacc cccgaggcgt
     6181 acgccgcgat gccgcacgac cgggagtatg ccgacggggt ggtgttcgcg gccgcgaacg
     6241 tctacgccct cgtgatcacg gccgattgcg ccgccgtcgg attctacgac cccgtgcgcc
     6301 aggcagccgg gaacgcccac gtcgggctcc tcggggccgt gaacgaactt ccgaagggga
     6361 tggtcgcggc gctggtcgag agtttcggca gccgcccggc cgatatcgag gcggtgatat
     6421 ttccttgcat ccggccctgc cattatgcaa ccgccaactc ccaaacctgg ctgcgcatcc
     6481 gcgaacgcgt gcacgccgcc ttcggagagg acaatcccct gtttgcctcg gggtacttcg
     6541 acctgcccgg catggtgcgc tcccaactgc gcgcctcggg gctgctcgag gagcacatct
     6601 tcgacgtcgg cctgtgcacg gtgtgcaacg ccgataccct cttctcccac gtggccgccg
     6661 gatcggccga ggcccaggcg cgggaaggac gcttcggggc ggtcatgggg ttccgccccg
     6721 aggcgccggg cggtcccaca ggcggtggcc ggtgagcggg aattttgttg acgcccggtg
     6781 cgggcaggtg tacccatgag ctccatgaat cacgatgatc cgccccccgg ccttcccgaa
     6841 cggctcctcc gctaccatct gccggtgctc ctctacgccg gcgggataat cgccctctct
     6901 tcgatcagag actttcgccc tcccgatttg ccgatcccct cgcttgacaa ggtcgtccat
     6961 ctgctggagt acgcagtgtt tgcgtacctc gtgtttcggt cgttctacca tatggggcgc
     7021 tctccgcgac tcggccgggc cctgctgtgg tctgcacttt ttgtaacttt ctttgcactg
     7081 gcggatgaaa tgtatcagcg ctatgtgccg gggcgccatt ccgactggcg ggattttgtc
     7141 gtcgatgtcc tcggggcgct cctggtgctg gggatactcg ggatctaccg gtggcggagg
     7201 cggcgcagat cttattaaga tttttcgggc gcgccggccg tgaacctttt tagtcaattc
     7261 tcttgacaat cagcttggcg gccatatatt tggtagagtc tgatagcgac tgaacgggta
     7321 gtcgtctgtg gcggacaccg ttttcaaatt ggtccaacta gagcgttgaa acgcaaggtc
     7381 tgcacaaaaa gggaaggtcg gtcggaccac ggagcagagc ggccggcaga agatccggtc
     7441 gtggaacgcc atttgcgtgc aggactctga atgatcctgc gccggctaac ggtatccgca
     7501 gcgaggcaga cgcaaggaca gacgaagctt tgcgcggggt tgtagagaga actcaccgga
     7561 taccgacgga gtgaggcggc ctgccgaatt gcggatggcg gcggagagat tgcgcggccg
     7621 acgtcccgca tggaacgaag aaaacaaaaa cgcgaatttg cgaacaatca acaagtttat
     7681 ggtaccagac gttcagagtg aagggagagc agaagtatga aattgcgcct actcatggct
     7741 cttgttgtgg cactcgggct ctcggccggg tcggtcctgg cccaggcgcc gcccccacag
     7801 gatccattcg ggaccatgga tacggtcgcc ctggtggcgg ttggcgatcc cgccagcggc
     7861 catttccagg tggagctgtg ggtgtacagc gacgaagata tcggtggggc gaccaccggg
     7921 ttcgtttggg acaatgcgaa agtgcagatg gacagcgcca aggcttcggc actgacggcc
     7981 ggcgctttca ctctcggaac attcttctac gagcagtcga atatcaccct gaccaacagc
     8041 aaccggcgat tcctcttcgg cggtttggct ttcacttcgg cgctgcccgg cgacgtgtca
     8101 actcgccgtc tctgggcgac gtactacttc aacaccacgg gctggacggg atctgataac
     8161 gtcaccttcg agacccaggt ctggggtatc gggaccgact attcgtacgt cgagttcaac
     8221 acgaacgagg aattctatcc ggtgtggatg ggggccctca caattccacc tccgtcgcta
     8281 aatgacccgc cggtgctggc ggcgatcgga gcgaagtcag tcaacgaggg ggcgctgctg
     8341 tcgttcggcg tcagcgccac cgatccgaac ggcgacgcca tcactctcac ggcgagcccg
     8401 ctgccgacgg gagcgaattt caccgacaac ggcaacggga cggggacctt cagttggact
     8461 ccgaatttca cgcaggcgaa cgtctacaac atcaagttca aagccgtgga ctcgaagaac
     8521 gcgtcagaca gcgagattgt cgcgatcacc gtcaacaatg taaatcaggc gccggtgctg
     8581 aatgcgattg ggccgaagtc ggttgacgag ggcgcgctgc tcaccttccc cgtgagcgcg
     8641 agcgatccgg atgggacgat cccggcgctc tcggcgacca atcttcccgc cggggcaagc
     8701 ttcaccgata acggcaacgg gacggggact ttcagttgga ccccgaactt cacgcaggcg
     8761 ggcgtcttca acgtgacgtt tgcggcgagc gacggcgtgt tgacggatga cgagatcgtc
     8821 gcgatcacgg tcagcaacgt gaaccaggcg ccggtgctgg cggccatcgg gccgcagtcg
     8881 acgccggttg aggtgctgct atcgtttggc gtgagtgcaa ccgatccgga cggcacgatc
     8941 ccgacgctgt cggcgacgaa tcttccgacc ggcgcaagct tcaccgataa cggcgacggg
     9001 accgggacgt tcgactggac gccggcgatc ggccaggaag gcgtgcacaa tgtgacgttc
     9061 acggcgagcg acggcgtgtt gacggacgac gaaatcgtcg cgatcacggt gacgggcggc
     9121 gcgaacgagg cgccggtgct caacgcgatc ggcggcaaga cgatcgacga gaacgcgctt
     9181 ttgcagttca ccgtgtcggc gagcgatccc gacggcggtt tcccggagct gacggccgag
     9241 gaccttccga cgggcgcgac ttttgtcgac agcctgaacg gggtcggcct gttcacctgg
     9301 acgccggatt tcacgcaggc gggtgtctac aacgtgcgct tcatcgccag cgacggcctg
     9361 ctggccgatt ccgaactggt cgagatcacg gtgaacaacg tgaaccggcc gccggtgttg
     9421 attacgatgg cggaagacac gacgatcgac gaatgcggct cggcgtcgtt ctggttcgac
     9481 gcgaccgacc ctgatggcgg ggtgctgacg ttctcggcgg acgggctcgt cgcgaacatg
     9541 gcgttcgatc agctcgattc cggggcgctg ttcacgttca atccggacac gacgcaggcc
     9601 ggttcctaca cggtgacggt gcgcgtgcag gatgccttgc tggcgaccga ctccgtgata
     9661 ttcacgatca cggttgagga gtgcacaact ccgccgccgt gtgtcgacct tattctctcc
     9721 gacacggttc ttttcatcca tgacacgatc acctttgcgg acgggcccta cggcttccgc
     9781 gtggtgacgg taggcagcac cgggcccggc gccgggtgct tcatcgtgct cgacacgacg
     9841 gcctggctga gcgcgacgcc gccggagacg tgcaccccgg cgaatgtgac gatcagctat
     9901 gacgctaccg gcctgccggc gggcgactac tacggcatcg tcatgttcca gggcgacgcg
     9961 accgtgtgcg agccgcgcac ctactacgtc atggtggcgt tgcagctggt ggacacgacg
    10021 acggttcccg aaggagccga cacgttgttt gtcgggatcg tgcctgcggc gccgggcgcc
    10081 gaggtggtgg tgccgatgga attcaccaac tcgtgcgatc tgttcagcgc gcaggcgacc
    10141 ctggagtgga acccggccta cctgacgctt gactcggtcg ggttcgaggg ctcgcgcctc
    10201 gacgatttcc cggtgaaggc ggttgatatt ttcgccggcc atgcggtcat ttcggccgat
    10261 ttcggcgggt ctgcgccgat ggtcggcccc gggttcggcg gcttcgcgaa cctctacctg
    10321 accgtggcgc cgggtgcgcc tgtaggcttc cacgaaatct cgatcgtgac tccgacggag
    10381 gacacgtatt tcgggatcaa ctgcggcggt ccggacctgt tcatcccgcc cgttccggag
    10441 aacggcggcg tggcgatcga tacctcgacg gcgttcacct gcggctacgt ggtcgacact
    10501 gccggcgacc cgatcgccgg cgcgaccgtc gagatgtggt cgggtttccc cggcatgggc
    10561 ccggaagcct cgacgacctc cggggccgat ggttactttg aattcactac tgagcttgtc
    10621 tacggccagt tcgacctgtg ggcctacaaa gagggttact accccgggct catcgaaaac
    10681 gtgaactggg gcgagacggg catcatgatt gtgctgacgc cctacgcgcc ggtctacccg
    10741 accctctact gggtcgactt ctactgcgac gcgaacacct atttcgggtc gccgctgccg
    10801 atcggatcgg tgattgacgc ctatgatccg gacggcgtgc ggtgcggctc gtggttcgtg
    10861 aaggaggccg gcaagtacgg cttcatgccg gtgtacggcg aggaagagtg gaacgagggc
    10921 gaccagggcg ccgagccggg cgacgaaatc cggttcttca tcaacggcgt cgaagcggag
    10981 gcgactggca acaccctgtg ggccaacgca ccgacggttc cccagatggt ctgcctgaac
    11041 ctcgacgtct gccgccaggt gtgcgatctc acggctggct ggaacctcgt gagctggtcg
    11101 atcgacaccg aatccgacga catcgaggcg gccctggcct cgatcgcgcc ccacatccag
    11161 ctcgtcctgg gcttcgaaca gggcggcctc acctacgatc cgacgctgcc ccagttctcg
    11221 acgctgtggt atgtggacca cctctcggga tactggatca aggtggacgc ggacgtcgag
    11281 atggtgatcg agggcaactg cgcgaatgtg tcgacgccga tcacggtgac gccgggctgg
    11341 aacctcgtgt cgtacctgcc ccacgtggcg atgccgaccc ccgacgcgct gacgtcgatc
    11401 tacgacgatc tcatggtggc gctggggttt gacggcggcg ccctgatcta cgtgccgagc
    11461 gacagcgggt tcaacacgct cgacgagatg gcgccgtgct tcgggtactg ggtgaaagtc
    11521 gacgcccctg gcgagctgat ctacccgtcg gacgggccgg tcgtgttcgc tcagcagcgc
    11581 cagccgctcg gacatcccct gcccaaggcg gccgagggtg tcacggcgac gacgcagtgg
    11641 atgaacctct acgcgcagaa gctgacggtt gacggcgcgg tggtcccgac gggggcccgg
    11701 gtcgaggcgt tcacgctcga cgggcaccgg atcggcagca gccaggtgcg ggccgacggt
    11761 ttgttcgggt ttatgccggt atacgccgac gacccgaaca cgagcgcggt tgacggtctc
    11821 cggacgggcg agccgttcac gctgacggtc aatggcgcgg ccactgccga gaccttcgtc
    11881 ttcacaggcc acggcaccaa tcttgaggtg agcggcctga cggcgaaggc gggcgaggag
    11941 ccgacgatgc cggggaccta cgcactggcg cagaactatc cgaacccgtt caacccgacg
    12001 accacgatcg agttcagttt gcccaaggag acccgggcca aggtcgaggt gttcaacgtg
    12061 ctgggcgagc tggtggcgac gccgtttgac ggcaccgccg cggccggcac cacctcggtg
    12121 acctgggacg gccgttcgcg gacgggctcc ccggtggcct cgggtgtgta cttctatcgt
    12181 ctcagtgccg ataactacac cgagacgaag aagatgatgc tgatgaagta ggcggcgatc
    12241 ctgcttcctc agaatgtcga gtagaaaccg gccggccgcg ggcatccgcg gccggtcggc
    12301 gcagagaagg tgctatggtg aaaagacgat tcgtgcggct cctgattgtg gcggcggcgg
    12361 ccgcgctccc gacgagcccg ctctgggcgc aggggatcat cccgacgccc tactcggacc
    12421 agttcaaagg cacggtgatg atcaacggcc agcccgcgcc ggtcgggacc gtgatcgacg
    12481 cctttgaccc ggacggggtg aacaatgggc gcgacaccac ccgcttcggc gggctgttcg
    12541 gctacttcgc cgcgtacggc gatgacgtgt cgaatacgcc ggcgctcgac gagggggcgc
    12601 gcgaaaacga cccgattacg ttccgaattc tcgggcggcc ggcggcgatc gacagcggcg
    12661 acgccacctg gaacaacatg gacaccagca ccgtgcacct ctcggtgtcc ggggtgacga
    12721 tcggcctgct gctggtcgac gcccccctga cgggagtcgg ctccccccgg gatacggtcc
    12781 ggttcgaagt gggcatccgc aacaccggca acatccgcga cttctacgac atcatcgttg
    12841 agcagtccaa agtgccggcc tggggggtca tctacccggc ggagttggtc tacaacggcc
    12901 cggattccac cgcctacctg tggttcgatg tcgtggtggc ggatttcccc ggcgaaccgc
    12961 cgctcaacca cctgacctac acggtgcggt cgcggatcga caccacggcc acggcctcgg
    13021 ggacggtgga tctcgatgtc gacgtggtcg gcacggagga ccgcgacgag ctgctcccgg
    13081 gtgccttcgc cctcgcccag aactacccga acccgttcaa cccgacgacg accatcgcct
    13141 tcaccctgcc ccgcgcttcg acggtgcggc tggaggtcta caataccctg gggcgcctcg
    13201 tagaggagcg cgacctggga acctacccgg ttggggacca cgcggtggct ttcgatggaa
    13261 cctcgttggc cagcggcgtg tacctctacc gcgtggcgac cgagtatggg tcggcgtcgc
    13321 ggaagatggt cctgatgaaa taggggcggg atcaagagtg ccagttgaaa aaaaccccgg
    13381 ttgacaccgg ggtttttttg tgcttttata gatgctcggt gcgacctttc cggcccggcc
    13441 gtacagccgg gcggtcgccc ggatgagaac tgccgaagag agaggaatgc gacgtggcgc
    13501 tcgtgataca ggcggtgggg aaaaccgatg tggggctggt ccggaaaggg aacgaagaca
    13561 acctgtatct ggatgaccgg aaccacctgt acgttgtgtg cgacggcatg gggggccacc
    13621 aggcgggcga ggtcgcgtcg atgtcggcgg tcacgactct cgagaccgtg cacaaccagc
    13681 tccggcggga gattctcggc gacgagcggc tcagccccgg gcgcgcgctt cccgacgaaa
    13741 ccaacctcct cctgcgcgcg atccgcctgg ccaaccggga gatctacggc aaggcggccg
    13801 cgaactccag catggcgggc atgggcacca cgattgtcgc gatcgccttc gagcacgacc
    13861 tggcgggcat cgcccacgtg ggcgacagcc gcgcctatcg cctcgaggcg cgccgcctcg
    13921 tccctctgac cgcggaccac tcctgggtga gcgagatgca ggccgcccac aacctgtcgg
    13981 cgcaggaagc ggagtcgctg gtcgggcgga acgtcatcac gcgcgccctc ggcgtgcgcg
    14041 aggcggtcga ggtcgattgc cgcctcgtcc gggtaaaacc gggcgacatt ctcctgatgt
    14101 gctccgacgg actctgcggg tttgccgacg acgatgagat tttcgaggcc gcccaccggt
    14161 tgcgcgacga cctgaagaag atggtggacg ccctcgtgca gttcgccaat gaccgcggcg
    14221 gcaacgacaa cgtgacggtg gtggcgatgc gggtgatgga ggccaaagcg accgatatcc
    14281 ccgaactgcc ggtgttcacg ctgaccgccg agtcgcgcga ggtgctggcg gccgaggatg
    14341 agtggctgcc gaaaatccgc gaggcggcgg aggccttgca cgccggcgga aaacgccaaa
    14401 aaaaaaagta ggccgctcct cgggtggctt tcggggctgg tggtgatctg gggggtggtg
    14461 gcggcggttc tcggcgccgc ccgcgggcgc tgattctcac gtcaattcag gtgaagaccg
    14521 cgccgggaga cggccggggg cggggaaaaa agccttgaca ttccggggcg gcggcgttac
    14581 taattgaacc gcaccgacaa agtggaaatg cgcccatagc tcaattggat agagcgtctg
    14641 actacggatc agaaggttgg gggttcaagt cctcctgggc gtattttgtt ttgttcggtc
    14701 gcgcgctgga gccgccgcgc gagcgaatcg ccgcgccccg cgcccttcag tccttcacgc
    14761 agcgcacggc attcccgctc ttcttgccgc tgtaatcgtg caccatctcg gggcggttgt
    14821 agcccaggtt gcggttccag gcctgatcgg agaagtactg gtgcggggtg gcggtccaga
    14881 acccggcgcc atagtggatt ccgttgaagt cacccatccc gccgcgatag ccgcccggga
    14941 gcccggagaa gccgctctcg ttggtggcgc cggcgttcgg atcggcccag tacgcagtgc
    15001 ccgcggcttt cagtttcccg ccggccaccg caccgccgcc gagcgagtcg accagttcct
    15061 gccactcgga atcggtggcc acgtgccacc cggcgggagc caaaccgcgg gcgtcgccga
    15121 ccgcaaacca gttgtacagc cgaccgtaga ttgccacatt ggccgggtcg ttggcgtact
    15181 cgcaacaggc gccgctcgcc agagcattcc aggcggtggg atcggtaacg tcggaaacgg
    15241 ggtcgccgtt gcggtaatgc gtgaccttga ggttctcggc catccaccac cggttgaaga
    15301 tcttgaccgt ttggtaggtg ttgccgtcgt agtcggcgac ggttcccaat gtcgcgaacg
    15361 gcacgtcctc gccgtagccg gtgccggcgc ggttggtcgc atacgcccgc atgtgatagc
    15421 tctctccgcc gctcaatccg gtcagagagc ttgtgaaact tccgaccccg atgccgtcgg
    15481 acgtggtgtc gtcggcaatc gtcgggtcgg cgctcgtgcg ccagcacacg ccgcgggcgg
    15541 tcacttcggc gccgccatcg gcggtgaccg tcccgccgca ggtcgccgtc gtggacccaa
    15601 cggcgctgac cgcggacgtg gtcacggtcg ggattgtcgg ttggtcgggg ttggtggaat
    15661 cgtcgtcccc cgagcatgcc cagaacacca gggccgttgc cgccaggacg ccggccggca
    15721 gccaggtcgc tttcatgata tcacctccgt cttgaccgta acagccgcac tcagcgaaac
    15781 aaacacgcgg cgagctcagt tgtcaagcgc ggcgagccgg caaatgacgg ccccgggagt
    15841 ggggacccgc gaaggcgtca gagctcctgg gagctgaacg tgtccccctg ggtgatatcg
    15901 ccggattcgt atccccgtcg gaaccaccgg acgcgctgct ccgaggtgcc gtgcgtgaaa
    15961 gcatcgggga cgacataccc ctgggtttgc ctcatgatgc ggtcgtcgcc gacggcggcc
    16021 gccgcgttaa tgccctcttc gatgtctccc gactccagga gattggcggt gcgctccgca
    16081 aagtgggccc acacgccggc caggaaatcg gcctgaagtt ccatccggac cgtgagccgg
    16141 ttgaactccg cctcgctcat gcgctcccgc tggcccatca cttggtcgga gataccgagg
    16201 aggttctgca catggtggcc gacctcgtgg gcaatgacat aagcgagcgc gaaatcgccg
    16261 tgggcgccga gctgcgcctg catttccttg aagaagctca ggtcgatgta gacgcgctcg
    16321 tcggccgggc agtagaacgg tcctgcggaa gcgccggcat aaccgcaggc cgacgagatc
    16381 tggtctgaga agagcacgag tttgggctcg cggtaggact gcccggcctg ctgaaagaga
    16441 cgattccaga catcttcagt ctcggccaga atgacgccga cgaactggcc ctcctgccgc
    16501 tcctcatcgc taagctccgc cgtgctcccg gcgggcactt ccactcccgt ctgctgcagc
    16561 gactgcatca cttcatcggg attgcccccg aggacaagga agaggacgac ggcgagaatc
    16621 ccgcccagac ccccgcccac ggcgagacct cggccgccga ttccccgccg gtcttcgacg
    16681 ttatcactgg ttcgccgacc ctgccatctc atatcgcaat cctttcgttg cggccgttca
    16741 tgcgatcggg ccatcgcgga tcgcccattg cggctggccg cgccgggctc tggagtcatt
    16801 atagcctgcg cggtcctcat tgtcaaacgc tcgctcgtcg cagccgcgcg cgtcgcatcc
    16861 gcctgagtca gctcaggcgg gcatgtccga ctgcgcctcc ggcggagtgc gaaccgtctg
    16921 ggccagggcg aaatcgacgg cggcgcggca atggagacgg agagtgtcga tcaggggaat
    16981 ttcgacatcc tcaggcgcga cgagcagcgg cagttccgta cagcccagga cgacggcctc
    17041 cgcgccgccg tcggcgaggc cggcgatgat cctccggaac cggtctttcg cccgatcgga
    17101 gaccacgttt ttcagaagct cggtcagaat ggtcgtgtgg atgaatgcgc aatcgtcgcg
    17161 gtcgggcacc cgcacgcgga tgccgcggcg ccggagaacg tctttgtaga agttctcctc
    17221 catcgtcatg cgggtgccga ggagaccgac ggtggagaaa tgccggcggc tgatttcatc
    17281 cgcggcggcc tcggcgacgt tgacgagggg gaggcgggtg caacccgcga gctcctctgc
    17341 gaattggtgc agggtgttgg cgcacaggac aaggcactcg gccccggcgc gttcgaggga
    17401 atcggcggcg gcgcgcacca gcccaaaaac cccccgggcg tcccccgccc ggttcagcgc
    17461 atcaatgtcg gcgtaatcga acgaatagag gagacagcgg gcggcgtgga ggccgccccg
    17521 ccgctcgttg gtcagctcgt tgatgaggcg gtagtaggcg caggtggaca cccatcccgt
    17581 gccgccgatc aaaccgatca ctttcatctc ggccgccgtc cgcgtcagag aatgcggttg
    17641 ccccggtagc ggaagcagtc gccgccgttt tcggccaggc ccgtctgcac cgtcaggccg
    17701 ctccgcttca gaccgtcgct gaacgcccgg aaccggtact cgctgttcat cgcgtgctgc
    17761 gggaagtaca ccgccggcga gagtttctgc agggcgtaca caacgccctc gcgcactgat
    17821 tcgggggtcc ccaggctgca gcccagaatc ggcagaaagg cgaggtcgat gggggcgccg
    17881 agagaggcga ggtaatcgat ttcatcggtg tagggcgcat ggagaccgac ctggccgttg
    17941 gcgtggtccc cggcgtgaaa gatcaccacc ccgtcgacct ccaccaggaa cccgaccccg
    18001 gcgtcggttg cgtcaatcgt ccggacgcgc atgccgtcga tcacctcatc gctccgcgga
    18061 cccaggtagc gatgcgccgg agcgccgtcc tggcggtggc cgagcacgta gctgatgtcc
    18121 ggaatcgcgt tccgccaggc gaaaatcgaa gtgtcgtagt ggtcgcggtg ttcgtgcgag
    18181 gagaacacca tcaccttgag atccttgatc tcagacgggt cgatccggcc gttggcgagg
    18241 cagggcgaat cgggattgca cgggggaagg gcgtaatcga agatgaggaa gtggcgggcc
    18301 gtccggatcg cccagccgga gtggttcagg taccacacca ccgcttcttt ctcacccagg
    18361 gtcgccgcca gcaggtccgg gggatcggcc tcgctctcgg ccgggagcgg cgccgcgccg
    18421 gcgtccctca ggagagcggc caccgacggg ttggcgtagt gaacggcgta gtagagcggc
    18481 gtgcgcccgc tccggtcctg ggcggccggc ggcacgccgt ggtcgagaag cacccgtgcc
    18541 agggcggagt cgccgcggat cgcggcgatg tggagcatgg tccggccgta gtcggcatcg
    18601 gtgaactcgg ggtccgcccc ggcctgaagg agaatcccgg cgatttcgtc gttcccctgc
    18661 actgcggcgg actggagcgc gggaccggac agccgcccgt ggtaattcac gtctgcgccg
    18721 cggccgacaa gcaggcgcac gaggtcggcg tggcccgcca gcaccgcgat cgacagcggc
    18781 gtgtgcgtcg agtcggtgcg ggcgttgacg tcggctccgt gatcgagcaa ccaccgcacc
    18841 atgtcgacgg tcccccagca ggcgttggtg agcggcgtca cgccccagtt gtcgcgcgcg
    18901 tcgatggcca gcccggccgc gaccagcgcc ccggccagca ccgtgtcgcc gcggaaaacc
    18961 gcggtgtgga ggcgggtttc gccctgctcg ttgcggtact ggacgtcggc tccctgggcg
    19021 atcaggcgga gggcgatgga cgtgaaaccg ttggcggcgg cgaaatagag cgcgtcgcgt
    19081 ccgtcgccgt tgcgcgcatt aatgtcgaac tcctgggcga ggaggtacgc cgccagcgag
    19141 tcggcgccga tcgaggcggc gatgtgcagg cagttggagc cgcgcgcgct cgtctcgtac
    19201 aggggcgcgc cgtgcgcaac cagacagcgc accatctcga ggttcccccg ccgcgccgcc
    19261 gagatcagcg gggtctcgcc gttgatgtcc cgggcggctg cctgggcgcc ccgctcggcc
    19321 aagaggcgga cggcgtccag gtggttgcgc atcgccgcga tcgtcagcgg gctcgtgttc
    19381 tcgcggtcgc cgatgttgat gtccgctccc tcggcgagca ggagttcgat catggcggtg
    19441 cggccctgga gcgccgcgac gtggaggggc aaattctggg cgctgtcgcg cgcattgatc
    19501 agggacggat cggcggccag cagaacgcga acgcgctcga catcgccggc cgcgaccgca
    19561 tcgtggatct cggccgccgg cagccggact gccgccagca gaaccacggc ggcgcaaagg
    19621 gcaaaggttc ttcctgtcat atctgtcctg cccatggata gggtcctcgg gacgaggcgt
    19681 tttgtttaaa tgttatgacg cggggcaaag cggattgttc agtctatcac cgcgccggcg
    19741 tcggcgtact ttctgccccg gcaggaaaca gccccgctct ctgcattcag gcgggagcga
    19801 acgtctttaa tgcggactat ttgtatacgg attggcacgc cggcgccgcc aggatggcac
    19861 gaccggacgg gcgccgccgg gcgagccgcg cccggcggcg aatcagtctg gactcaaggc
    19921 cggccgctag cggacagcct ggcggtaaat ggcatcgatg atctcggccg gcgaccgcgg
    19981 cgccgacaca gcggcctcat cgggaaactc cgccctcgcc ttgtccggtg cgttccagcc
    20041 ccagatgaag gtgtgtgcag gcgcgctgaa actgatcagg cgtccggtca gccagccgcc
    20101 ggagaagttg ctccccaccg atggattcgg gccggtaaaa ccggaaagct cgaccgaaat
    20161 gagctggagg gaatccggat aaaggaccgg tcgcaccgtg taggacgtgt cggctgtcca
    20221 gtccgtgacc agggactggc gggtcacggc gttggcgcga ttgaagaacg taagcatgag
    20281 ggcgtaaaac tgggcgtgct cgatccgctg ccagacgatg gtcaccgatt cgccgggggc
    20341 caccgtgtcc tcgccgggaa cagggctgat gatctgcgct tcagaatcgc ggggatcgag
    20401 catgaccaca tcgcacaccg cctggcggcc gggtccccaa atctcaaggc gggcgacgtc
    20461 gcctgaagaa tacaggaagg agccgccggc cgattcgctg aaccctatct gccagtggcg
    20521 acggccgggg gcgtcctgga accagttgta gtccgtgggc cgcgtcatga gcgaatcgcc
    20581 gaggcggatg gaatcgatgc tcggaacaat acccccataa ccgtagataa cggcgttcac
    20641 gatagcctgg gggttgactg ttatgctgcc gtcaacaaaa cccgccggcg tcgccgggca
    20701 gtcggcgcag ctctcgtcgt cgctgcagcc gagcggcagc agcaggaaga cagccagggc
    20761 tgaaagaacg atcaaaacgc gctgcatgag aagcctcctt gtgatgagcc cggctcgcgg
    20821 catttcccac ggcgtgcgac cgggccgcaa gttacgctca agaacgaaac atggcgcgga
    20881 gggggacagc aaggaaaatc ggcggcgagt tcttctgtaa tcaatcggtg gccagcccgg
    20941 ccaacagcag gccgccgggc cgaaacccgg cggcggtgcg cgtggtgcga ggacgagaag
    21001 cagcagtcta ctttgcggcc tggcgataga ttccgtcaat gatctcggcg ggggagagcc
    21061 gttgcgaagc ggctaggtca tccggtagcg tcttgatcga cttgtccggc tcattccagc
    21121 catgtagcat tgtgctcgcg gggccgccga aactgatgag tcgccccgcc agccacccgc
    21181 cggcgaagtt gcttccgaca gccgggttcg ggcctgtaaa gggtgtgatt tcaactatga
    21241 gagccgccag agaatcgggg tacagggttg gaccaacagt ataggcagtg tcggcggtcc
    21301 agtcggtgac attcgattcg cgggtcacgc cgttggcgaa atcgatgaat ctgagttgta
    21361 ccgcgtagta ctgggcgtcc agcgctcgat gccagatgac ggcggccgac tcatccgggg
    21421 caatggtctc cgttccggga agagggctga tcatatcggc agcggactca ttgggatcga
    21481 gcacgaccac atcgcagtaa gccaagcggc cgggtcccca gatctcgatg tgcgccacgt
    21541 cgccgggcga atacatgacg tcgccgctga tgttctcgta gaaggatatg cgccagagag
    21601 gattcgcggg agcgctcatc gcaacatagt aatcgccgga cgacgtcagg agcgaatcac
    21661 ccagccggat ggagtcgacc gttgggagga tgctcccgta accgttgatc gtggccgaca
    21721 acgacacctg cggagtcacg tgaactgtgc cgttaacgaa gcctgcggga gtcgcgtgct
    21781 cgggacagtc cgcgcagtca tcgctgcagc cgagcggcag caggctgacc gccacagcgg
    21841 agagaacaag aacgatgcgc tgcatgagaa atctcctggt gatgagttgt atctacaccg
    21901 gtttcggaga aaccgaaccg aacggcaaac cacatgatag aacgagaccg cctgcccctc
    21961 cggcgagcaa gataaaacgg cggccattcc gtctctgacc ggtttcaccg cacggatttg
    22021 ccacttcacc gccggatggg agtaaacagg cggcggtgaa tttgccgcga gagacaggca
    22081 gttactcggc agcctgccgg tagactgcct cgacaatctc ggcgggcgtc cgctgcggga
    22141 agagcgtctc cccggccggg agtgccgact tcgtaatcgg gggaggattc caccctacga
    22201 tggccgtctc agccccgtac cctacactga caatccgtcc ggacaaccac gccccgagta
    22261 cgttgctgcc ggcggcgggg ttggggccgg tgaacgggac cacccgaacc gagaactgag
    22321 cgagtgaggg aggatacaga ctgggcgaca cagtgaacga ggtgtcggcg gtccagtcga
    22381 accggtcgga gaacgtgaaa acatcgccca cgcggttggc aaggatgacc tgcacggcat
    22441 agaactgggc atgctccacg cggttccaga acacgatggc gggttcaccc ggggcgacgg
    22501 tatcggtccc ggggtaggga tcggtgatga ataactgcgt cagattgggg tcgaggagcg
    22561 ggagatcaca agtggcgaga cgcccctcac tccagaattc gaggtgggcc gtatcgcctt
    22621 gggtatacat gtagtccggg ccggtgtctt cggtgcagta gatcgtccag taggggttga
    22681 ccggctcgct ccgatacgag tagaagaaat ccttgttaaa aaccagtagc gaatcgccga
    22741 cgcgcgctga gtcgagcgtg ggcaggatac tcccatcgcc gaaaatcaag gcttcaaagt
    22801 aaacgcccgg gtgcaggtca atgctgcctt gaatgaaccc gaccggcgtc acgggcgccg
    22861 gacactccgc gcagtcgtca tcgctgcacc cgaggggcaa cagtgcgacc accacggcgg
    22921 aaaggacgat catggtccgc ttcatgagtt acctcctgtg aaggatatat ggccgagatg
    22981 gtttccgtga aacagtaccg ttcaacggaa tctcctgcgg aaaaatgaat tcgcgccacg
    23041 agttgcggca acacaaaaac gcggccgggc acaacggcgc agcgggttgt cgccgcagcg
    23101 ccgcggttac cggggtgcag agagcgtttc agtctgcgcc tgatcgccgg tcgccgatgg
    23161 catccggcgg caatagtgct cgtagcgggc gaagacgtcc cggacgaagt cggccgtctc
    23221 ggcgctgcga aggtagccga agcggagggt ctcatcctcg accgcggggg agtccgctct
    23281 gagccgcagg taatcttcca cgatgtcagt ccagaagcgg acgtcttcgc cgtagcgttc
    23341 ggcgagccgc cgggcctcct ggacgcgcgc cgcgccggcg ttgtaggcgg ccaacgcgaa
    23401 cttcaggcgc tccacggtat cgggcacgtc ggagaactgc ctctccagga agtccaggta
    23461 ggcggcgccc gcggcgatgc tgcgggcggg atcgaagatg tcgtcgccga tgacgccgaa
    23521 gtgggcggcg gtcgagggga gcagttgcat gaggccggcc gcgccggcct gcgactctgc
    23581 ctcgggatcg aactgcgatt cctcgtagat caccgcggcc agcagccgcc agtcccaacc
    23641 gatctccgcc gcatggcgcc ggatgagtcc atcatacggc gaaaggcggt cgctctcgac
    23701 cgttgcccag gcgccgacgg ggcgctgccg gaacgccttg cgattccggt agtacttgtt
    23761 gtagaccgcg tagtagtcgg gggtccgctt gaactgccgg agccaggtgt tcagcgcggc
    23821 gagcagcagg ggagaggact tgctcacggc ccaggcgatc tgctggggcg gcccgaccgc
    23881 ggtgccgaca tcgagattcg ggtaatcggc ggcgatgatg tgcgccagat tctgatcgga
    23941 gaccgtgtaa gtgatgtcgc cggcggccac ccggcggatg agctcctcgg tgggcacgcg
    24001 gccgtcgacg atttcaatgt cgacggcgcc cgcgatctct ccggcgagct cgacgagccg
    24061 ctcgtagtag gctgaaccgt gccggacgtg cacggtccgg ccggcgagcg agtcgggcgc
    24121 gcggatcagg cacgactcca gaacccgcgg gtccatgcga cgccacagtt cgggcttgcg
    24181 ctggaccagc acctggtggg tcgaggtgag cggctcggcg aacgccacct gcgcggcgcg
    24241 ctcggcggtg accgtaagat tgtaggccac gagatccccc tcgcccgccg cgagcagaat
    24301 gatcaagctg tcgatatccc ggacggggat gacctcgagc cggagatcga gatgttcggc
    24361 caggcgggcg agcagctcgt gttcgaaccc catggtgcgg ccgcggtaga cgaagtaccc
    24421 ggtggaactg aaagctgtga ggacgcggag aacgccccgg cgcttgatct gcggaaggtc
    24481 ggcggggtgg cggtcggccg actcgagggt gcggtcacag ccggcgcagg cggccgccag
    24541 gacgagggcg gcgaggagcg cgcgcttccc gcatgcgcgg ccggtcgatg ccatcttcgg
    24601 gcgcctgggc ggtttccact tcatgccaat gcctaccgga gcaaccgggt gtcctccggt
    24661 ccaatctacc gggcgccgcg cggccgggca atttatttgt gcggggaatc ggggcgcggc
    24721 cgatccggcc ggccccccgg tcgagccggg cgctccatgg cgtggaggcc ggcgaggcgg
    24781 acggcgcccc ggtggtagcc gcccggaaac aactgctcca gctcgtcgag ctccagtccg
    24841 ttcgcctcgc acgtctcgta caccgtcggc accgtcccgg tccgcccgta cgccccccga
    24901 aggtaccgaa tgatctgcca gtggcgctcg gtaaggttgc cggaaatgcc cgactcgtaa
    24961 gccttgtgca cggcgaaatt ctcgtcccag tcgtcgggat tcatcaggaa tccgtgctcg
    25021 tccagctcat aggtcctgcg ctcgtaggtg ttcgtgaagt gggcgatgtc gccctccagc
    25081 cagacgtgct ggagataccc ctcgcggtag gtgacgccgg cgagcttgca ggcgccgcgc
    25141 agccagccgg tggggaagag cttcttgagg tcgcccaggc cgaggtggtt tttcttgcag
    25201 gcgacgtaaa tgagggggca ctggttgatc tgctcaaacg tgttgcggat gaaacggatt
    25261 acgcgccagt gggcctcggt caggccgcgc tcgatccccg cctcccgggc catggcttcg
    25321 gcaaaaccct catcccattc ccccggatcg agcaggaacc ccttgtcgtc gacgcgataa
    25381 tccttgccgt tggccgaaaa cgtcttcatg gccccatcca atgccgcaac tcacgcgctg
    25441 cggggcgttc gggcggagac cagccgggcc gccctccgaa ttccacttca tggtacgata
    25501 aagcgaacgc cttgacaatg gttttgtcgg gactcagcgc ggctgggaga cgtcgatcaa
    25561 cacctcgccg cgcgtcacgc ggacgcgcaa cccatcggcg gcgacagcgt agatccgccg
    25621 cacctgttcg cctacggcgg cgaagcggaa cgtgcggctg ctctccgggc cggccgcggt
    25681 ctccgcgatc gccttcccgt cgcgcaggag ttccacgacg cactcggcgc ccgaggaatc
    25741 gagtccgatg agccccacct caaccgcgag cgcggtgtcg aggcggacga ggaccgagtc
    25801 gcccgctttc gccccgcggc gcgggtctcg cggcggcggc gacggctcgg cctgaagcga
    25861 atagtcgagc gggatgacaa agagggcgga atcaaccggg ccgattgcga tgttggtgac
    25921 ggccacgtac gcggagtccg gggacatgag gttctcgatg cggatcggga agccaagccg
    25981 cgtggggatc cactggttca tgagcgggtt cttgccgagt cggattgtga agcggtcaca
    26041 gggaaacccc tccagcgtgt cggcgccgcg gtagtcgcgt acgcccagcg agtcgccaat
    26101 ccgggcggcc tggaagggat cgttctgcac aaccgtcgga gcgttcagcg gcagaatccg
    26161 gtaggtccgc gccggcgggt tgacaatccg gcactccccc gcgctctggt cgaccagcac
    26221 gtacactttg cccgcatcct ggctgaaatc catgcggtag agggagtctt tgacgaagag
    26281 ccggcccgtc atcgtctggc gtgcgctctt cagagtaagg tcgccgacaa actcggcggc
    26341 cccagccgtc ggcaccgaca gcaggaccaa agcggctgtc gccgcgaact tccgcacaag
    26401 cggcgagaca agctgcacca tgaacacgtc tccgtttcgg ttaagggcga tcctcttctc
    26461 caattccgcc ttcggcggcg gcaaagtcaa cggcaaagat cgtcccggga cttgtcgctt
    26521 tatacccctg aacgggccgg ccggacggga gattcggatg gagcgcggtc gccgacaaac
    26581 cgctggtcgc cggtgccgac gcgcgggggg cgcatccgcg gcggaatgac ttcggctgcg
    26641 gcccaggtca tgtgaaaccc tttcgcccgg ccggaaaaaa gcgttgtctt ttgccaccat
    26701 ttgcccctta attcgtttaa aggagaatca acgtaaacgt aggcaacagg ttgagaatat
    26761 tggaccagca gcaaaaggcg cccgcccacg tccccgtgcg taggccactc cgccacccgg
    26821 tacttccggc gatcctggcg gcaattatcc tcaccggcct gcctgcggcc gcgacgcacg
    26881 ccgtggccgg atggccggtc ggctatgacg tcgacctctc cagcgggttc ggggattacc
    26941 ggttcggaca cttccacttc ggcatcgata tccgcaccgg cggcgtaacc ggcaaagagg
    27001 tgctcgcccc ggtcgacggc tacgtctggc ggctgcggac ttcctacacc ggctacggga
    27061 aagtgctcta tctccggggg aatgacgggt gcacctatgt catggcgcac cttcaggatt
    27121 tcaactccgc cctcgaccgg cgcctgaaaa cggcccaggt gagcgccaag cggtattacc
    27181 aggatctgta ttttccggaa gactcggtcc cggtgaagaa gggggagttg ctggcctatt
    27241 cggggcaaac cgggtccggg gcgccgcacc tgcatttcga gaagcgtgcg ggggacatcc
    27301 ccctcaaccc gctgtcgcac ggttaccggc tggccgacaa gacgccgccg gtcatcaccc
    27361 ggctggggct gcggctgacc gatgaccaca gcg
//
LOCUS       DAOPOE010000038        27222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2260_length_27222_cov_5.424964, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000038 DAOPOE010000000
VERSION     DAOPOE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_27222_cov_5.424964"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(427..2595)
                     /locus_tag="PKM94_06775"
     CDS             complement(427..2595)
                     /locus_tag="PKM94_06775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD66381.1"
                     /translation="MVDRSRGFFDRWGLLVVLAVALLVRVVYLFQYHATGDWSQLTVD
                     ANYHHHWAQDIAAGDVFGDTTYFRAPFYVFCLALLYAVLGDSLWIGRLFGLAIGLASV
                     ALTSLLARRLFGAKAGFFAGLVHALCMMAVYFEPELLVDPLYTLLLLAALHGFVTWYD
                     SRAVRDALTFAVLLGLAAITRPNALVMVPVAVLVLAVRWRRIAAPVPHLGALALGLLL
                     VIGPITLRNYLKADDPALIATSGGINLYIGNNPEADGAAAVLPPPMGPYWRLEQTEYI
                     AESDLGRDLTPGEVSAYWTDRALAWMKDNPGAFLRLYARKFFLFFSNAPVSNNRDLEP
                     FISRIAVLRLNPVRFALVFGLAVAGLAAGALASRPLRWILLFVALSILSGAVFFYATR
                     FRLPLEPVYFALAGFALARLPDLWRESRLRCGAALGGGILAALVSFALPGPPVTGYSI
                     HPLVEAGGYEYQKGNYVQAWRHYQMAAAVDSVYPGVNTNIGSVLLALEQADSAEVYFQ
                     REVRFNPWYAPGWTSLAHVAMARGDLRGAIGMARQALARAPYAAGPNLVLVRALAADT
                     AVSDDSLAGEIARAAQRARDDIYLLQMGAELLLNRGALAPASRFARRALEAEGPPIEM
                     DHEAFRHDFRNSRDKLNSQRAAAAVQLAYIAGNGNDIESAIAFARRAVELAPHQAEGY
                     LNLYSGLTAAGRTAEAGALLKEMAERFPDNRFVREMLRRR"
     gene            complement(2694..3230)
                     /locus_tag="PKM94_06780"
     CDS             complement(2694..3230)
                     /locus_tag="PKM94_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66382.1"
                     /translation="MNLIGLVIAVALLAFVHESLHAATAALFGEYGSFRVHWYGFEVI
                     YRTAVELRAGIHWALIAGLPNLVTVLLGYLLLTGRSVFARIAPLAGRSLAYWATLIFL
                     LADPLNIAVGPFLYGGDAGGIAHGLGLNQYFLQALAFGVFLWNRELVASRLLPAFGVV
                     TSHPLFRPWLSGGPAAGE"
     gene            complement(3414..4259)
                     /locus_tag="PKM94_06785"
     CDS             complement(3414..4259)
                     /locus_tag="PKM94_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOD66383.1"
                     /translation="MFDKSDLNEYLRRQVFVAFDTETTGLWAPVNRVVEIGAVRFELE
                     VEGGRSFESLINPERTMPLDVIRVHGITDQMVAEAPLAADILKQFKEFCGSDTVLIAH
                     NAPFDISFLGMEFSRAGISYPENIIVDTVDILHRTVPGLPSYSLEALARRFRFAQVQE
                     HRALADALLVAALFRLAAPGLEGIGKKEDWGRNLAAYRMAEWHDDSIRLPEEFAALDR
                     AVRLNQRITIVYEARPNAPQRRTIQPRRIHRLRNAYYIEGWCEKARADRTFRLDRITR
                     YTLEE"
     gene            4694..6217
                     /locus_tag="PKM94_06790"
     CDS             4694..6217
                     /locus_tag="PKM94_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOD66384.1"
                     /translation="MPIVLNGSGLTIEKLVRIARHNEAVELAPEALERIKACRAMLER
                     KIEAREIMYGINTGIGEFSEVVLSDDQVQDFQKYLIYNHAAGIGEPMGLEAVRGAMAS
                     RVNVHAHGNSGCRPEITQTLVAMLNRGVTPYVCLKGSVGACGDLAPMAQIALLMMGEG
                     KAYYRGELLPGRTALERAGIPIPGLKARDGLATINGSNVMTAKAALFLHDANRWLKQA
                     EIACAMSLEALRANMKPYTPKLHEIRGFAGAVRSAKAIRKVVEGGDLAEGRVPCKVQD
                     AYSMRSTPQVIGAAHDALAYARAQVEIELNGVGDNPIFWPEENLQLSGANFQGTPVSV
                     PMDMAGYCITMVSVLSERRMNRLNNPALSVGLPPFLTKGAGMFSGLMLSQYTADSLIV
                     EQRILSMPASVQSIPAAADQEDFVSMGMNTAIKNDQILENAYGVLGIEFMAAAQALDF
                     RDYKFGVGTRTAHDIVRRHVAFLDIDRPLYPDHTAMREVVRSCEILDGVEAAVGSLG"
     gene            complement(6312..6737)
                     /locus_tag="PKM94_06795"
     CDS             complement(6312..6737)
                     /locus_tag="PKM94_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66385.1"
                     /translation="MRTLTKEDVMFTRRFAALTLGAALVSLAAAPGVMSKDEGASAAP
                     AAAPAAAEGKTWYLVISPHTQEGCLAALDSVAAEGAAVLQQFDWGCMAGDHTGYARVQ
                     AAGKAEALKMVPASHRSKARAIELNKFTLDQIRAFHESH"
     gene            7194..7994
                     /locus_tag="PKM94_06800"
     CDS             7194..7994
                     /locus_tag="PKM94_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66386.1"
                     /translation="MANRCAWVFGAALAAAVTAAGCGGGGTLAIVADRSDGLAADGAA
                     RDWDALSARYIEADRVVVGAATDGERLFVLLRFRDPQYAAQIRSEGLTLALDPTGGFG
                     ERFVLHYRGMPAGTDGVPAALQAELRRLRSAAPGDTLRAEELRIAIRDEIETKPIPAT
                     GEEGPSAAAGFDQGFFVYEFQVPLGKSAVRQYRVPAAPDAEVGVVLEWGGRAEADLLN
                     EIAGGGLMVDGPLFPTADEPPRRDGAEDPGKSRLGRHEIRLRVSLGGR"
     gene            complement(7991..9994)
                     /locus_tag="PKM94_06805"
     CDS             complement(7991..9994)
                     /locus_tag="PKM94_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOD66387.1"
                     /translation="MADSPTDTPRSPLAPPRAVAFLFGAYLVGLIVFAAARLVFLLHF
                     PHEVRAAGLGPLLLAFAVGLRFDTVVIAAVLLPLVAGLPFLMRRPRLGLAIIYLVAVC
                     SVLFLLLLGDIRFFEEFDNHLNFHVIDYLTEGRTAWNLVLFDSSFWSLTGVWVALAGL
                     LTLGLLVVRRLVRPLPLVRRWPVATGYTVLLVLLAALGIRGRVDLAPIDWGVAYFSHN
                     HFINQLALNGVYTLGRTATEHDGRLSALRPPDRWEFTEFSEAIDTVRAMLDLPGDTWL
                     EPGRSLRRTTHQPEPKFGFRPNIVLVLMESFSGRNTGVLGSPENLTPCFDSLARRGIL
                     FSNFFATGTRTSYGIAGAIGSFPSVPGRSILKRYEARHPFVMLSEILRRRGYVNAFIY
                     GGDLVFDNMGGFLRTKAYDRILGEEDLGRERSFSKWGIPDHLLLRSALELADTLPRPF
                     QLTILTLSNHSPFELPDSSVRRYQGDDRLSRIYNAQIYADWAIGRFMAEAADRPVFDS
                     TIFVFTADHALWGVASNSLSPVNLHIPLLIYGPALLGDSGRTIATYSGQCDLLPTLMG
                     LLGGDYTHESWGRDILRVPPADSGFAIFNSSYRIGYLVPDFFLLEHLGGKPGLFDPDF
                     LGQEEAMVNEQYPDVLRRLQRRLHYYLQAAEQATLPEAQRRGL"
     gene            complement(10061..10381)
                     /locus_tag="PKM94_06810"
     CDS             complement(10061..10381)
                     /locus_tag="PKM94_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOD66388.1"
                     /translation="MIVTTSEFIAHKRIAKTLGLVRGNTIRARHLGHDIAALFRNIIG
                     GEVNNYTKLIAEAREQALDRMIEEARGLGANGIVAARFATAEIMAGAAEIVAYGTAVV
                     LEDE"
     gene            complement(10378..10821)
                     /locus_tag="PKM94_06815"
     CDS             complement(10378..10821)
                     /locus_tag="PKM94_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD66389.1"
                     /translation="MRNEIRELLVGYLDGELSDEERTAVEAALAEDPDLRAELEEFRK
                     LKEVTGMAQYADLPPQVWEQYWESLYRKLERGAGWIFLSLGAIVLLGFGLYEAFRGMY
                     YDPLIPLWVKVGVSVGAAGVVILLVSYGRERYFAFTRDRYRRVLR"
     gene            complement(10814..11362)
                     /locus_tag="PKM94_06820"
     CDS             complement(10814..11362)
                     /locus_tag="PKM94_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD66390.1"
                     /translation="MRTDTTLISACVAGDKKAFGQLVERHKKQAYLWALGLVGNKDDA
                     YDISQEAFLRAYRSLDRFDRTRPFAPWFYAIVANLSRSWLRRRALQNRRSVDLDDVSY
                     LLTTESTPEQEYARKALVGQLRAALMRLPYNDREIIVLQHFRNMSYDEIAELLGIPRG
                     TVMSRLYYARKKLATEMRHCHA"
     gene            complement(11588..12112)
                     /locus_tag="PKM94_06825"
     CDS             complement(11588..12112)
                     /locus_tag="PKM94_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD66391.1"
                     /translation="MRVFLSACVLAGALALVIGCSSPQTAKIGDRVKIEYVGKTDDGV
                     VFDSTTAGQGIDFVIGQRQVIPGFENAVVGMAVGETKSVSIPPEEAYGPHRPELTHTY
                     PADSFPDNIQPELGMQLQMQTPDGQPVTLVITAIDRDSNVTVDANHHLAGKTLLFDLK
                     LVEIMAVAGDGAEE"
     gene            complement(12330..12797)
                     /gene="mscL"
                     /locus_tag="PKM94_06830"
     CDS             complement(12330..12797)
                     /gene="mscL"
                     /locus_tag="PKM94_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOD66392.1"
                     /translation="MIKEFKEFAMRGNVVDMAVGIIIGAAFGSIVKSLVDDVIMPPIG
                     LLLGNVDFSSLFVTLKEGATAGPYATPALAKAAGAVTLNYGLFINSIISFLIVAFAVF
                     LLVKNVNRLKSKMETPAPAAVPTTRECPFCLSTIPKAATRCPHCTSQVQADKV"
     gene            complement(12956..14590)
                     /gene="recN"
                     /locus_tag="PKM94_06835"
     CDS             complement(12956..14590)
                     /gene="recN"
                     /locus_tag="PKM94_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOD66393.1"
                     /translation="MLRSLTIRNVALIDDAELEFGPGLIALTGETGAGKSVIVTALAL
                     ALGGRADREFIRHGSPTAAIRAVFEDPATRTRCEVFREIAETAGTSKLTIDGAPAAVA
                     QLRERAAPMAEILGQHAGQMLMNEDNHLLFLDRFAGLEPGRGEAGRLFREWRETAEEL
                     RRVRSRRDQLAAERELLMFQQAEIEKAQVRAGEEEGLERERRILDAGRSLMESATRIT
                     DAIGGDDESILDRLQLARRELDKMAAVDPALEKQAETLAEIDFQLEDLRRHLEQYGAS
                     IEDNPARLEEINFRLDELYGLKRKYGGSEEAVLAALERIREQLKERPDTDSLIAALER
                     QAEQCAATYADHALRLTAARRQAAADLRTLVIEELAELAVDHASFLCDFAYEEDEGGV
                     ILGDRAVRPHETGLELVRFLFSANPGEPLRSLVKTASGGEISRVLLALKSAELRHGRL
                     GPGLLVFDEVDAGIGGRTANEVARKLKKLSVSCQVLVITHLHQIARLADQHFVAEKTV
                     ARGKGKRTVITVRRLDGAAIRQEIDRMVALPEPPGR"
     gene            complement(14590..16317)
                     /gene="dnaG"
                     /locus_tag="PKM94_06840"
     CDS             complement(14590..16317)
                     /gene="dnaG"
                     /locus_tag="PKM94_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD66394.1"
                     /translation="MIAPDTIEQVRQATDIVALIGQYVRLKRQGRCLVAVCPFHTEKT
                     PSFSVSPDRQMYYCFGCQRGGTVFTFLQEHEGMSFVEAVQFLAQKANIHIREDRERDG
                     RREASEKIAYANSVALEFFQKQLFNPRYRAALEDYLMKRRGITRDTIREFQLGLAGEE
                     WDSLLRFARPKGLSPEDLVECGLALYSDKSRQHYDRFRQRLMIPIFNLSQKPIAFGGR
                     TLKKGETVKYMNSPETALYSKGSVLYNLNFARDPIRSENAVFVVEGYFDVISLWQAGV
                     KNVVASSGTAFSLPQARLLARFAESVYLFFDADSAGRAAALRSVDVLYDAGLEVKVVV
                     APPGEDPDSVARAHGREKIEQLRRAAVGYITYRTQDIDVRRAGIIGREKLVKEFAALG
                     ARIADPTRRSLFWEEAATVLGLNPDLLISAAPREAAAAETAPAKRARKYDPLALHLLS
                     LMLHNPGAIDAVFERVAPEDFEAPELTRLYAAMARQYRVTGEIDARGLVDTAQDEAFQ
                     SLIAAVAAIEWEPDKVEAEARKMLKEFLTQRSRASLPELKRQLAEAEAAGDREAIARL
                     MAEIHRRVQ"
     gene            complement(16321..18681)
                     /locus_tag="PKM94_06845"
     CDS             complement(16321..18681)
                     /locus_tag="PKM94_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOD66395.1"
                     /translation="MIDQHTLDILEYPKVIAQVAGRCLTPYGADEVNRIRPLYDPAEI
                     ERRGSEISQMRDIVSFGMAFPLARMENCRPELLRAQVEGVFLDPEQILRVLELVEVSI
                     ALNGYDREGRPKFPLIAAYLEQVRAFPELKAEIRRAIDENGEVKDSASPKLKQVRAEF
                     GDAKRRLVARLTAIQADRPHQAGVQDDIVTMRNGRYVITVPSSSYRANMGILHDRSQT
                     GATLYIEPPETVELNNRLNLLQQEERLEIDRILRAITAEIARRGEALLENTRLIGRLD
                     SLYACAKYSKAVGGNKPALGPESSLSLVDARHPLLAVQFGKPEAVVANSLALDDLRQA
                     ILVTGPNTGGKTICLKTVGLAVLMAQSGLHIAAADKSSVGIFEYVCADIGDEQSIELS
                     LSTFSSHVRNLIDGVRKASPRTLLLFDEIGAGTDPKEGAALAEAIILHAIRKGARMIV
                     TTHFSQLKTLPMDNPEIENASFEFDRTTLAPTYRLQLGTPGSSYAIEIASRLGMSEEI
                     TERASRLVGSGEKSLAALISSLESELAVVRKDRAELTDRLAEARRLEDTYRSRVDELT
                     TNVEETRRRALAETEAFIEQTRREVEQLVADIRRSQAEPQQVKNFHRSLRERAKELSS
                     LKKASETPAPEPGRFEKGDRVEIVSLGQAGEIDELLGDDRARVKVRNVFTTVEIRHLR
                     RLEEGGAPARPEATGARVSARPVESPEIHLRGLTGDEAIEELERYLDRAVVAGLHQVY
                     VVHGKGTGALRKRITEYLREHPEVASVRLGNWNEGGAGVTIVKLKE"
     gene            complement(18772..19404)
                     /locus_tag="PKM94_06850"
     CDS             complement(18772..19404)
                     /locus_tag="PKM94_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOD66396.1"
                     /translation="MNWVDIVLFVLLLAMVIVGSKKGLVRELMAFLVFLIAIVVSVNY
                     IDRFAVWVYEKVGGSPLVAAFLSFAVLIALSYAAFKLMGMVFYKVANIKSIGKKDQMG
                     GALIGFLRGWLLVGFVTLLLFLLPMPASFYTAFEQSFFGPMVAKTVPLVFEGTAPVHP
                     RNRTFIAKIETALLTAQTSNKKTTDAQRSEVYEVLHQMRRFFATSDPAQP"
     gene            complement(19470..19916)
                     /locus_tag="PKM94_06855"
     CDS             complement(19470..19916)
                     /locus_tag="PKM94_06855"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOD66397.1"
                     /translation="MSLLETIDKDLTSALKAGDRHKATVLRGLKSDLKYKKIDKGAEL
                     THEDIIAVLSTAAKKRRESIEQFQRGNRMDLVEQEQRELDLITAYLPRQLSEDDLRTL
                     IAEAIRETGAQSAADLGRVMKALMPKVRGQADGKLVNQLAAKMLAK"
     gene            complement(19924..20121)
                     /gene="rpsU"
                     /locus_tag="PKM94_06860"
     CDS             complement(19924..20121)
                     /gene="rpsU"
                     /locus_tag="PKM94_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOD66398.1"
                     /translation="MPGVRVRDDESFERALRRFNKFCEKTGVLSDIKKHQHYEKPSEA
                     KKRKMAAARRKQRRLRRLSRY"
     gene            20337..21173
                     /locus_tag="PKM94_06865"
     CDS             20337..21173
                     /locus_tag="PKM94_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HOD66399.1"
                     /translation="MPFTVAHAAAGLPLKAVLPRRLSLSGLMAGAMAPDLLYFLVMTT
                     VDRGLSHSWPGLFLFCLPAGTAFALAFHGLIKYPLLTNLPSPWDRRFSGLATARFHPH
                     GVRGWSVVVLSVGIGALTHFFWDSFTHPGGELARALPFLQAQVRAFGQVIPVSRIAQH
                     TSTLLGISALLSGSWRGWLLPAPATGARFRTARDKWRFWLGSGLFATLFALAVTWAYR
                     TYWPDRVATTFTTFGLAGWAGFFWVVAVCGAISRFLPGRGRSGPAGDRARQPVGAWRS
                     QS"
     gene            complement(21246..21578)
                     /locus_tag="PKM94_06870"
     CDS             complement(21246..21578)
                     /locus_tag="PKM94_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOD66400.1"
                     /translation="MASIFNRIIRREIPAKVFYEDDDCIVIADHRPKDRVHLLIIPKT
                     ETPSFFEAPPEVLAMLDEKTKLVARRLGLENHFRIQINNGYGQEIPHLHYHFLSNEGS
                     ERVHFVEE"
     gene            21827..22576
                     /locus_tag="PKM94_06875"
     CDS             21827..22576
                     /locus_tag="PKM94_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOD66401.1"
                     /translation="MSGHSKWATIKRKKGKLDAERGKIFTRHIKEIVTAAREGGGDPD
                     GNPRLRTAIQAAKASNMPADNIKRAIMKGTGELPGVSYESVVYEGYGPAGVAIYLEVL
                     TDNRNRTVSEIRHFLTKYGGNLGENGCVGWMFHKKGVIEIDKTAADEDTVMEIALENG
                     AEDVRTDSGSFEVVTPAEALETVRAAFEKKGVPMASVEVTMIPETTVRLEKEGEASSM
                     LKLYELLEEHDDIQKVYSNFDIDERLMEQLA"
     gene            23048..23326
                     /locus_tag="PKM94_06880"
     CDS             23048..23326
                     /locus_tag="PKM94_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66402.1"
                     /translation="MQCNSPRGSELPTDLSSVGERYVLEADSSGLCSSKDGLLHVDFT
                     FSTKPNSLTFTQGIAHPTYWHSVTTSTLTLKTLDGTGYFAVQFLLARQ"
     gene            23438..24229
                     /locus_tag="PKM94_06885"
     CDS             23438..24229
                     /locus_tag="PKM94_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HOD66403.1"
                     /translation="MTRGRLQGISGILCDIDGVLWDSQDRKRPTPGAHEAIRCIKEER
                     RLPVRFVTNTTTRSRDTLYREIRQRGYPIERDEIVAPTTLGAEWLRRKGRPSVFLVMR
                     EDTQEEFAEFPRDDVRPDYIVIGNNRDRWDYALVNRIFLMLVNGSEMLALHKQRYWQS
                     GDDIAVDIGCFVTGLEYSSGKRATAIGKPEPLFFQAALEAIGVAPAEAIMVGDDIETD
                     IGGAQRAGLRGVLVRTGKFREYLVRQSTVAPDLVIASLGDLPSHL"
     gene            24321..24812
                     /gene="ruvC"
                     /locus_tag="PKM94_06890"
     CDS             24321..24812
                     /gene="ruvC"
                     /locus_tag="PKM94_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOD66404.1"
                     /translation="MRVLGIDPGLQITGYGVIEDGGGAPVVLEAGVIRTTSGHDMSLR
                     LHELSCEIKGVIEQFRPDIIAVEELYSHYGHPRTAIIMGHARGIVFLKAAEAGVAVVP
                     YASTRIKKSLTGNGRASKRQMQMMIRSALHLPRPPEPPDAADALAVALCHCRAAAHHE
                     VAV"
     gene            24809..25456
                     /locus_tag="PKM94_06895"
     CDS             24809..25456
                     /locus_tag="PKM94_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOD66405.1"
                     /translation="MISRISGRLVNIENDTALVETGGLAYEIMLPSALAERLRDDGKV
                     GGDISLETLYYIDAGERKSAHFPRLVGFVNPIDREFFSVLTHVSGLGVKKVLKSLVMP
                     IRDIATAIENKDAAQLNRLPGIGARLANKIIAELHGKVAKFALSKEDQALSKAAEPVE
                     PLGEEARAVLLQLGYSRSEADRMVEGALKHGLKAARVEDLITVIFRNEQQKKAAE"
     gene            25456..26484
                     /gene="ruvB"
                     /locus_tag="PKM94_06900"
     CDS             25456..26484
                     /gene="ruvB"
                     /locus_tag="PKM94_06900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOD66406.1"
                     /translation="MARERIVSGEQIIPDEDKALVTLRPSRLSEYIGQNKLRNKLKVT
                     LDAARGRGEAVEHMLFYGPPGLGKTTLAYIIANEMGTKLVATAGPALQRAGDLMGILT
                     NLNEGDILFIDEIHRLTPVIEEFIYPAMEDFKVDFVVDKGAFAKVINVPLKPFTLIGA
                     TTRAGFLSAPLRDRFGLYYHLDFYPPEELADIVLRSARLLEVEIETEAAATIARRARG
                     TPRVANRLLRRVRDFAEVKAAGVITPEVAAAALDAEGVDSLGLDALDRNLIRVIIEFY
                     HGGPVGIEALGATLNEEINTLVDMVEPYLLKIGFVRRTRQGRVASEAAAKHLGVTLPR
                     TGRQGRLL"
     gene            26496..27191
                     /locus_tag="PKM94_06905"
     CDS             26496..27191
                     /locus_tag="PKM94_06905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOD66407.1"
                     /translation="MQSEPWIEVDRYLTALHVPPDPVLESALAASREAGLPSIQVTPL
                     QGQLLQVLALACGARRILEIGTLGGYSTIWLARALPPGGRLVTLEVNSSHAEVARANI
                     ARAGLAGTVEIRVGPALASLEAIAARGEGPFDLTFIDADKEGTPEYFRRALDLSRVGG
                     FIVVDNAVRHGKVVERDSDDPRVAGMRRFLELAAAEPRVKAATVQTVGAKGYDGFTLA
                     LVTAERRGGGGNG"
BASE COUNT         4842 a         8302 c         9448 g         4630 t
ORIGIN      
        1 ccctcgtccg gtgccgggaa atcaggggag aaaatcgttt ctctggtcgg gttggtggga
       61 aatggttgtg tgaaatgact tcggcgttta ttatcaagga gttacgggga gcgcgggctt
      121 tgagactgtt ttgcggtagg tcgtcgttcc ctccaccaaa tcccgcgacc gggcccggcg
      181 acaaatccgg gcccggtctt tttttgtgca cggggagaaa aagatcgagt tttccgctca
      241 agtcggcgcc ccggggcgcc gatacttctt atggagaatg accagggccc gctggtcggg
      301 ccgcgcggga agtgccccgg agccgcaagg cgccgggcgc ggagcgaaat gggcattctc
      361 taccgtaggg gcggattcat atccgccctt ctttttttgc ccatcggagg cagtgctgtg
      421 gggggctcag cggcgcctga gcatctcgcg gacaaagcgg ttgtcgggaa accgttccgc
      481 catctctttc agcagcgccc cggcttcggc cgtccgtccg gcggccgtca gacccgaata
      541 cagattcaag tatccctcgg cctggtgcgg cgccagctcg acggccctgc gggcaaaggc
      601 gatggccgac tcgatgtcgt tgccgttgcc ggcgatgtag gccagttgaa cagcggccgc
      661 ggcgcgctgg ctgttgagtt tgtcgcggct gttgcggaag tcgtggcgaa acgcctcgtg
      721 gtccatttcg atcggggggc cctccgcctc gagcgcgcgg cgggcgaaac ggctggcggg
      781 cgcgagtgcg ccccggttca aaagcagctc cgcccccatc tgcagcaggt agatgtcgtc
      841 ccgggcgcgc tgcgcggcgc gggcgatttc ccccgccagc gagtcatcgg acacggccgt
      901 gtcggcggcg agggcgcgca ccagaacgag gttgggaccg gcggcatagg gggcgcgggc
      961 gagcgcctgc cgggccatac cgatcgcgcc gcggagatcg ccgcgcgcca tggccacgtg
     1021 cgccaggctc gtccagccgg gagcgtacca cgggttgaaa cgcacctccc gctggaagta
     1081 cacctcggcg gagtccgcct gctcgagggc caggagcacg gacccgatgt tggtgttgac
     1141 gccggggtag accgagtcga cagccgccgc catctggtag tgccgccagg cctggacata
     1201 attccccttc tgatactcat acccgcccgc ctcgaccagc gggtggatgg agtagccggt
     1261 gaccggcggc ccgggcaggg cgaacgaaac cagggccgcg aggatgccgc ctccgagagc
     1321 ggcgccgcag cggagacggc tctcgcgcca gaggtcgggc agtcgggcga gggcgaaccc
     1381 ggccagcgcg aagtacaccg gttccagcgg caggcggaac cgggtcgcgt agaagaagac
     1441 cgcgccgctg aggatcgaca gggcgacaaa caggagtatc cagcgcagcg gccgcgacgc
     1501 aagcgcgccg gcggcgaggc cggccacggc cagaccgaag acgagcgcga agcggaccgg
     1561 gttgagacgg aggacggcga tccgactgat gaacggttcg aggtcgcgat tattggagac
     1621 cggagcgttg ctgaagaaga ggaagaattt ccgcgcgtag aggcggagga aggcgcccgg
     1681 gttgtctttc atccaggcca gtgcccgatc ggtccagtag gcggacacct cgcccggagt
     1741 gaggtcgcgg ccaaggtcgc tttcggcgat atactcggtc tgttcgagcc gccagtaggg
     1801 acccatgggc ggcggcagca ccgccgccgc accgtcggcc tcggggttgt tcccgatgta
     1861 gaggttgatg ccgcccgagg tggcgatgag agcgggatca tcggccttga ggtagttccg
     1921 gagcgtgatc gggccgatga cgaggagcaa accgagggcg agcgccccga ggtgggggac
     1981 cggggcggcg atgcggcgcc accggaccgc cagcacgaga accgccaccg gaaccatcac
     2041 gagggcgttg ggccgggtga tggcggcgag gccgaggagg acggcgaacg tgagggcatc
     2101 gcggacggcg cgcgagtcat accaggtcac gaagccgtgc aacgccgcca gcaggagaag
     2161 ggtgtagagc ggatcgacca gcagttccgg ctcgaaatag acggccatca tacagagagc
     2221 atgaaccagg ccggcgaaaa agccggcttt cgcgccgaag agccggcgcg ccagcagact
     2281 ggtgagagcg accgaggcca ggccgatcgc caggccgaag agccgcccga tccagagaga
     2341 gtcgcccaag accgcataca ggagggcgag gcagaacaca tagaaggggg cgcggaaata
     2401 ggtggtgtcg ccgaagacat cgccggcggc aatatcctgc gcccagtggt gatggtagtt
     2461 ggcgtcgacc gtgagctgcg accagtcgcc ggtggcgtgg tactggaaga gatagaccac
     2521 gcgcaccagg agggcgacag cgaggacgac gagaagcccc cagcggtcga aaaatcctcg
     2581 cgaccgatca accatgccgc aatataggcc ggttgttaaa ggaacgcatg catcaaattc
     2641 ggcgcggccc ggaccgcccc tgcggccggg gccgcaggcg gcggccgccg gctctattca
     2701 ccggccgctg gcccgccgga aagccagggg cggaaaaggg ggtggctggt gacgaccccg
     2761 aacgccggca agaggcggga ggcgaccagt tcccggttcc acaggaagac gccgaacgcg
     2821 agtgcctgga gaaagtactg gttgaggccg aggccatggg cgatcccgcc ggcgtccccc
     2881 ccgtacaaga agggcccgac cgcaatattc agcggatcgg ccagcagaaa aatgagagtc
     2941 gcccagtagg cgagggaacg cccggccagc ggcgcgattc gggcgaatac gctccgcccg
     3001 gtcagcagca ggtagcccag tagcacggtc acgaggttgg gcaggccggc gatgagcgcc
     3061 cagtgaattc ccgcgcgcaa ttcgaccgcg gtccggtaaa tcacttcaaa gccataccag
     3121 tgcacacgaa acgatccgta ttcgccaaac agcgccgccg tggccgcatg gaggctttcg
     3181 tggacgaagg cgagcagggc aacggcaatc acgagaccga tgagattcac cgtgagacct
     3241 cctccagcga gtagttgtcg tttaaaaagt ccgctcaatg atcatgtacg cggatgaagc
     3301 cgagatgttc aaatggggga ggcaatcggc ggtaattggg gcggaggaca gggggaaggt
     3361 tgacgaggcg atcccgtcat cgccggcagt gatgccgggc cggccgcccc tgttcactct
     3421 tccagcgtgt accgggtgat tcggtcgagg cggaacgtgc ggtcggctct ggctttctcg
     3481 caccagccct cgatgtagta agcgttgcgc agcctgtgaa tgcggcgcgg ctgaatcgtg
     3541 cggcgctgcg gcgcattcgg ccgcgcttcg taaacgatcg tgatccgctg attgaggcgg
     3601 accgcccggt caagagcggc gaactcctcg gggaggcgga tgctgtcgtc gtgccactca
     3661 gccatgcgat aggccgcgag gtttcttccc cagtcttcct tcttgccgat cccctcgaga
     3721 ccgggcgccg ccagacggaa caacgcggca accagaaggg catcggccag cgcccggtgc
     3781 tcctgaacct gcgcaaagcg aaaccggcgg gcgagcgcct cgagtgagta ggagggaagg
     3841 cccgggaccg tgcggtggag gatgtcgacc gtgtcgacaa tgatattctc cgggtaactg
     3901 atcccggcgc ggctgaactc catgccgaga aaggagatgt cgaacggggc gttgtgggcg
     3961 atcaggaccg tgtcgctccc gcagaattcc ttgaattgct tgaggatatc ggccgccagg
     4021 ggcgcctcgg cgaccatctg atcagtgatc ccgtgcaccc ggatgacatc gagcggcata
     4081 gtccgctcgg gattgatgag cgactcgaag gaccggcccc cctcgacctc gagctcgaac
     4141 ctcaccgccc cgatctcgac gacccggttg accggggccc agagaccggt ggtttcggta
     4201 tcgaacgcga caaacacctg gcggcgcagg tattcattca ggtctgactt gtcgaacatc
     4261 cggtcccttt cacctggagg tcgctcccgc agtatagacc atgcgaatca ggttgacaat
     4321 gccgtttctg catctatctt ggaggcgcca gaggcggaat tcactccctg gcggcgagag
     4381 tatgagctac cagacggcaa acatcacgct gcggactccc gatccgcgcc cggtcacgac
     4441 catcatgacc cggtaccagc cgacctccgc ctcgggtctc cccgacacct gagcggacaa
     4501 ttcacgcaca attgaggatc gagtcgccga tccgccgaag cggcgggcgt ggcgtgagtc
     4561 aatcagttcg cctccgcatg ggcgccggtc tggagcgccc ggcggggaag cgaatgtgtc
     4621 gggaccgcgg gagtcccggc ctgcagaaga tgagaccgcg aatcaatgat gtttgggaag
     4681 cagaggtttc gatatgccga ttgtgttgaa tgggtccggg ttgaccattg agaagctggt
     4741 gcggatcgcc cggcacaatg aagcggtgga actggcgccg gaggcgctcg agcgcatcaa
     4801 ggcctgccgg gcaatgctgg agcgcaagat cgaggcgcgc gagatcatgt acggcatcaa
     4861 cacgggaatc ggggagtttt cggaagttgt gctgtccgac gaccaggtgc aggattttca
     4921 gaagtacctg atttacaacc atgccgcagg gatcggggaa ccgatggggc tggaggcggt
     4981 gcgcggggcg atggcgtcgc gggtcaatgt ccacgcccac ggcaactcgg gctgccggcc
     5041 ggaaatcacc cagaccctcg tggccatgct caaccgggga gtcaccccct atgtctgtct
     5101 caagggatcg gtcggggcgt gcggcgatct cgcgccgatg gcgcagatcg ccctgctcat
     5161 gatgggagaa gggaaagcct actaccgcgg cgaactgctt ccggggcgga cggcgctgga
     5221 gcgggcgggg atcccgatcc cggggctgaa agcgcgcgac gggctggcga cgatcaacgg
     5281 gtcgaacgtg atgacggcca aggcggcgct gtttctgcac gacgccaacc ggtggctgaa
     5341 gcaggcggag atcgcctgcg cgatgtcgct ggaggcgctg cgggcgaaca tgaagccgta
     5401 cacgcccaag ctgcacgaga tccgcggatt cgcgggcgcc gtgcggtcgg cgaaagcgat
     5461 tcgcaaagtg gtggaagggg gcgacctggc cgagggacgg gttccctgca aggtgcagga
     5521 cgcctactcg atgcggtcga cgccgcaggt gatcggcgcg gctcacgacg ccctcgccta
     5581 cgcccgcgca caggtcgaga tcgaactcaa cggggtcggc gacaacccca ttttctggcc
     5641 ggaggagaat ctccagctct cgggcgcaaa tttccagggg acgcccgttt cggtgcccat
     5701 ggacatggcc ggatactgca tcaccatggt gtcggtcctc tcggagcgcc ggatgaaccg
     5761 tctgaacaac ccggccctgt cggtcggact gccccccttc ctcaccaaag gggcggggat
     5821 gttctccggg ctgatgctct cccagtacac ggccgacagc ctgattgtcg agcagcgaat
     5881 actctcgatg ccggcgtcgg tgcagtcgat cccggccgcc gccgatcagg aggatttcgt
     5941 ctcgatgggg atgaacaccg cgatcaagaa cgaccagatc ctggagaacg cctacggggt
     6001 gctggggatc gagttcatgg cggcggccca ggcgctcgat ttccgcgact acaagttcgg
     6061 cgtcggcacc cggaccgcgc atgacatcgt acgccgccac gtcgcgttcc tcgacatcga
     6121 ccggccgctc tacccggacc acacggcgat gcgggaggtg gtccggtcgt gcgagattct
     6181 ggacggtgtg gaggccgcgg tcggaagtct cggatagcgg cgccgccggg acgggcttat
     6241 gcctcaccgg gcgcggcagg ctcaggccgg gcctgccgcg cgcgggggtg aggcccttgc
     6301 ggcaaccgcc gctagtggct ctcgtggaaa gcgcggattt ggtcgagggt gaacttgttc
     6361 agctcaatcg cccgcgcctt ggaccggtgt gaagccggca ccattttcag cgcctccgct
     6421 ttgccggccg cctgcacccg ggcgtaaccg gtgtggtcgc ccgccatgca cccccagtcg
     6481 aattgctgca gcaccgcagc gccttcggcg gccacgctgt cgagcgccgc gagacaaccc
     6541 tcctgggtgt gcggagaaat cacgagatac cacgttttcc cctcggcagc ggccggcgcc
     6601 gccgccgggg cggccgatgc cccctcgtct ttgctcatga cgcccggggc cgccgccaat
     6661 gacaccagcg cggcgccgag ggtgagtgct gcaaacctcc gggtgaacat gacgtcctcc
     6721 ttggttaggg ttctcatacg aatgacaacc gatgtcgggc agttttgccg atgaatccgc
     6781 ctggcgcatc gccatcctgt cttgcgccca tactcgctcc cgggccggcc tggacagcgg
     6841 cccatatctc cgcatgcaaa agacactgac ggtgtgagtg cgccggacgc aggacggttg
     6901 accgtgccgg cgatactgga tagaattagt ctctgttggc ggattttgtc aagcgatatt
     6961 ttggggggct cccccggcgg ggtggcccgg tgcgggcgcc gccggagtcg gcccgccgac
     7021 tgccgtccgt tcggagggcg cccctcgctg tgccgcaaga ggttgcggtg gctccggcgc
     7081 accctccccg taggtctccg tcagcagcgg ttaaacattg cggaggtctg cggccaatgt
     7141 caaattgagg gggtgaacca gtgaatgacg tggattggga gacggagcag actatggcga
     7201 accgatgcgc atgggtgttc ggcgcggcgt tggcggcggc ggtgacggct gccgggtgcg
     7261 ggggaggcgg gaccctggct atcgtggccg accgcagcga cggcttggcg gccgatggcg
     7321 cggcgcggga ctgggatgcg ctcagcgcgc ggtacatcga agccgaccgc gtcgtcgtgg
     7381 gggcggccac cgacggcgaa cgcctgttcg tgctgctgcg gttccgcgac ccccagtacg
     7441 cggcccagat caggagcgag gggctgacgc tcgcgctgga tccgacgggg gggttcggcg
     7501 agcggttcgt gcttcactat cggggaatgc cggcgggcac cgacggcgtg ccggcggcgt
     7561 tgcaggccga gcttcgccgc ctgcggtcag cggcgcccgg cgacacgctc cgggcggaag
     7621 aacttcggat tgccatccgc gatgaaattg agaccaaacc gattccggcc accggggagg
     7681 agggaccatc ggccgccgcc ggtttcgacc agggtttctt cgtctacgag ttccaggtgc
     7741 cgctggggaa gtcggccgtt cggcagtacc gggtgcccgc ggcgccggat gcggaagtcg
     7801 gggtggtgct ggagtggggc gggcgggctg aggcggatct gctgaacgaa attgcgggcg
     7861 gcgggctcat ggtggacggt ccgctgtttc cgaccgccga cgagccgccg cgccgcgacg
     7921 gggcggagga tccgggcaaa tcgcgtctcg gccggcatga gattcgattg cgggtcagcc
     7981 tgggcgggcg ctaaagcccg cgccgctgcg cctccgggag ggtcgcctgc tcggcggcct
     8041 gcaggtagta gtgcagccgc ctctgcagac gccggagaac atcggggtac tgctcgttca
     8101 ccatggcctc ttcctgtccg agaaagtcgg ggtcgaacag gccgggcttg ccgccgaggt
     8161 gttccagcag aaagaagtcg gggacgagat accctatccg atacgagctg ttgaatatgg
     8221 cgaagccgga gtcggcgggg gggactcgga ggatgtcccg tccccagctc tcgtgcgtgt
     8281 agtcccctcc gaggagtccc atgagggtgg ggagcaggtc gcactgcccc gagtaggtgg
     8341 cgatcgtgcg ccccgagtcg cccagcagcg ccgggccgta gatgagcagg ggaatgtgga
     8401 ggttcacggg gctcagggag ttgctcgcca cgccccagag agcatggtcg gcggtgaaga
     8461 cgaagatggt gctgtcgaag accggccggt cggccgcctc tgccatgaac cggccgatcg
     8521 cccagtcggc atagatctgc gcattgtaga tccgcgagag ccggtcgtcc ccctgatagc
     8581 ggcgcacgga ggagtcgggc aattcaaacg gactgtgatt ggagagcgtc aggatggtca
     8641 gttgaaacgg ccgggggagg gtgtcggcca gctcgagcgc gctgcggagc aggaggtggt
     8701 ccggaatccc ccacttggag aacgaccgct cccgcccaag atcctcctcg ccgaggatgc
     8761 ggtcgtacgc tttcgtccgc agaaatcccc ccatgttgtc gaagacaagg tcgccgccgt
     8821 aaatgaaggc attgacgtag ccgcggcggc ggagaatctc cgacagcatc acgaaggggt
     8881 ggcgcgcttc atagcgcttg aggattgacc ggccggggac cgaaggaaag gaaccgatgg
     8941 cgccggcgat gccgtacgag gtccgcgtgc cggtcgcgaa gaaattcgaa aagaggatcc
     9001 cccggcgggc aagcgaatcg aaacagggcg tgaggttctc cggggagccg agcacgccgg
     9061 tattgcggcc ggagaaactc tccatgagga caaggacgat gttgggccgg aagccgaatt
     9121 tcggttccgg ctgatgcgtg gtccgccgga gcgaccggcc cggctccagc caggtgtcgc
     9181 cgggcaggtc gagcatggcc cggacggtgt cgatggcctc ggagaactcg gtgaactccc
     9241 agcggtcggg cggccggagg gcggagagcc ggccgtcgtg ctcggtcgcg gtgcgcccga
     9301 gggtgtacac gccgttgagg gcgagctggt tgatgaagtg attgtggctg aagtaggcca
     9361 ctccccagtc gatgggggcg agatccaccc ggccgcgaat acccagcgcc gccagcagca
     9421 cgagcagcac cgtgtagccg gtggcgaccg gccagcggcg caccagcggg agcggccgga
     9481 ccagccgccg gacgacgagc aggccgagcg tgagcagccc ggccagggcg acccacaccc
     9541 cggtcagcga ccagaacgag gaatcgaaca agacaaggtt ccaggccgtt cgcccctcgg
     9601 tgaggtagtc gatgacgtgg aaattcaggt ggttatcgaa ttcctcgaag aagcggatgt
     9661 cgccgagcag gaggaggaag aggacgctgc agacggccac caggtaaatg atggcgaggc
     9721 cgaggcgcgg gcggcgcatg agaaagggga gtcctgccac cagcggcagg agaacggcag
     9781 caatgaccac cgtgtcaaag cgcaggccga cggcgaaagc gaggaggagg ggaccgaggc
     9841 cggcggcgcg cacctcgtgc gggaaatgca gaaggaagac caggcgcgcg gccgcgaaca
     9901 cgatcagacc gaccaggtag gcgccgaaaa gaaacgccac ggcccggggc ggcgcgagcg
     9961 gggaacgcgg ggtgtcggtc gggctatcgg ccatgacgca attaacggcg cacgggcgtc
    10021 atgtcaagcc gccgcgcggc cggccccggc aaagaggcga ctactcgtcc tcaagaacga
    10081 ccgcggttcc ataggcgacg atctcggcgg cgccggccat tatctcggcc gtggcaaagc
    10141 gggcggcgac gatgccgttg gcgcccaggc cgcgggcttc ctcaatcatc cggtccagcg
    10201 cctgttcccg ggcctccgcg atcagcttcg tgtagttgtt gacttcgccg ccgatgatgt
    10261 tgcgaaagag ggcggcgatg tcgtggccga ggtggcgggc gcggatcgtg ttcccccgca
    10321 ccagcccgag cgtcttggca atgcgcttgt gcgcaatgaa ctccgaggta gtaactatca
    10381 cctgaggacc ctccgatacc ggtcgcgcgt gaacgcgaaa tagcgctccc gtccgtacga
    10441 caccagcagg atgacgacgc cggccgcgcc gacggagacg cccaccttga cccacagcgg
    10501 gatcagggga tcataataca tcccccggaa ggcttcgtaa aggccaaagc ccaggagaac
    10561 gatggcaccg agcgagagga atatccaccc cgccccgcgc tcgagtttgc ggtagagcga
    10621 ttcccaatac tgctcccaga cctgcggcgg caggtcggca tactgagcca taccggtcac
    10681 ctctttgagt ttgcggaatt cctccagctc ggcccgaaga tcggggtctt cggcaagggc
    10741 tgcctcgacc gctgtccgct cctcatcgga cagctcgccg tcgagatagc cgaccaggag
    10801 ctctcggatc tcgttacgca tggcagtgcc tcatttcggt ggcgagtttc tttcgggcgt
    10861 agtacaaccg cgacatgacc gtgccgcgcg ggattcccag cagctccgcg atctcgtcgt
    10921 acgacatgtt gcggaagtgc tggaggacga tgatctcgcg gtcgttgtag ggcagccgca
    10981 tgagcgccgc gcgcagttgc cccaccagcg ccttgcgggc gtactcctgt tcgggcgtgc
    11041 tctcggtggt gagcaggtag gagacgtcgt ccaggtcaac cgagcgacgg ttctgcagcg
    11101 cgcggcgccg cagccaggat cgggacaggt tggcgacaat ggcgtaaaac cacggcgcga
    11161 acggccgagt ccggtcgaaa cggtcgagcg accggtaggc ccgcagaaag gcctcctgcg
    11221 aaatgtcgta ggcgtcatcc ttgttgccga ccaaccccag cgcccagagg taggcctgct
    11281 ttttgtgccg ctccacgagc tgcccgaaag ctttcttatc tccggcgaca catgccgaaa
    11341 tcagggtagt gtccgtacgc atggcgacca tgtctcgttt caaccgtgcg attgttcctt
    11401 ccagatcatt acgaactggg gggcgggaat attcgaaaat cgcgccgcaa aggagcgacc
    11461 ggcgcgcggg ggggccgata ggccccgggg aagctcgcgg taacgtgcgg taacgcaatg
    11521 ggttggccgg ccgaacaaga aaacggggcg aaaccgcccc gtgagacaac ggattggctg
    11581 acgcgcccta ttcctcggcc ccgtcacccg cgaccgccat gatctccacc agcttcagat
    11641 caaacagaag ggtcttgccg gcgaggtggt gattggcatc gaccgtaaca ttgctgtccc
    11701 gatcgatggc ggtgatgacg agagtcaccg gctggccgtc gggcgtctgc atctggagct
    11761 gcatcccgag ctccggctgg atgttgtcgg ggaagctgtc ggccgggtag gtgtgggtga
    11821 gttcgggccg gtggggtccg taagcctctt ccggcgggat ggagacggac ttggtctcgc
    11881 cgacagccat tccgacgacg gcgttttcaa agccggggat cacctgccgt tggccgatga
    11941 cgaaatcgat gccctggccg gccgtcgtgc tgtcaaacac cacgccgtca tcggtctttc
    12001 ccacatattc gattttcacg cgatcgccga tcttggcggt ctgcgggctg ctgcagccga
    12061 tgacgagcgc gagcgcgcct gcgagaacgc atgccgacag gaaaactctc acaagaacct
    12121 cctcaggagt gtgaccggtt cgaggcccgc cgactgcggg aatgccgccg gcgccgattt
    12181 ctccaaaact acggtacgga ccgccatcgg gcaagaagat ttccggcgcc ggcacacggc
    12241 ggagcgccgg ccgattccgc ggccgcgaaa aagctgcggc gccgccccgg ccggagcggc
    12301 gccgtgcggt atcgagccga ggtgcgcaat cagaccttgt cggcctgcac ctgcgacgtg
    12361 cagtgcgggc agcgcgtcgc ggccttagga atggtcgaca gacagaacgg acactcccgg
    12421 gtggtcggga ccgccgccgg agccggcgtc tccatcttgc tcttgagacg gttgacattc
    12481 ttcacgagca ggaagacggc gaaagcgacg atgaggaagc tgatgatgct gttgatgaag
    12541 aggccgtagt tgagggtgac cgccccggcc gccttggcga gggcgggagt ggcgtagggt
    12601 ccggctgtcg caccctcctt gagcgtgaca aagaggctgg agaaatcgac attgccgagc
    12661 agcaagccga tcgggggcat gatgacgtca tcgacaagcg acttgactat cgagccgaac
    12721 gcggcgccga taatgatacc gaccgccatg tcgacgacat tgccgcgcat ggcgaattct
    12781 ttgaattctt tgatcatggc catgaactgt tctctccttc acataaaggg ccaaagaaca
    12841 caggcaacgg tgagccaaga gaaatatccg gagccgctcc tcggcgatgc aactgaaaac
    12901 ttgtctccgg ccgtccataa agctctccgg ccggcgggcg ccgggacggg agcgcttagc
    12961 ggcccggcgg ttcggggagg gcgaccatgc ggtcgatttc ctgccggatg gcggcgccgt
    13021 cgagccgccg caccgtgatc acggtccgct ttcccttgcc ccgggcgacg gtcttctccg
    13081 cgacgaagtg ctggtcggcc aggcgggcga tctggtgcag gtgcgtgatc accaggacct
    13141 ggcacgagac cgacagtttc ttcaatttgc gggccacctc gttggccgtc cggccgccga
    13201 tgccggcgtc cacctcgtcg aacacgagca gaccgggtcc cagccggcca tggcgaagtt
    13261 cggcggactt gagggccaaa aggacccgcg agatctcgcc gccggaagcg gtcttgacga
    13321 gcgaccggag gggttcgccg ggattggccg aaaacaggaa gcggaccagt tccaggccgg
    13381 tttcgtgcgg ccgcacggcg cgatcgccga ggatcacgcc cccctcgtcc tcttcgtatg
    13441 cgaagtcgca caggaaactg gcgtggtcga cggcgagctc cgcgagttcc tcaatgacga
    13501 gggtgcgcag atcggccgcc gcttggcggc gggcggccgt gagccggagg gcgtggtcgg
    13561 cgtatgttgc ggcgcactgt tcagcctgcc gttccagcgc cgcgatgagc gaatcggtgt
    13621 cgggacgttc cttgagttgt tcacgaatgc gctcgagcgc ggccagcacc gcctcctccg
    13681 acccgccgta tttcctctta aggccgtaga gctcatcgag gcggaagttg atttcctcga
    13741 gccgcgccgg gttgtcctcg atcgaagcgc cgtactgctc gaggtggcgg cgaaggtcct
    13801 cgagctggaa gtcgatctcg gcgagcgtct cggcctgctt ttccagggcg gggtcgaccg
    13861 cggccatctt atccagctcc cggcgcgcca gttggaggcg gtcgaggatg ctttcgtcgt
    13921 cgcccccgat cgcgtcggtg atcctggttg ccgattccat gaggctgcgg ccggcatcaa
    13981 ggatgcggcg ttcgcgctca agtccttctt cctcgccggc ccgcacctgc gccttctcga
    14041 tctccgcctg ctggaacatc aggagctcgc gctcggcggc cagttggtcg cggcggctgc
    14101 gcacgcggcg cagttcctcg gccgtctcgc gccactcgcg gaacagccgc cccgcctcgc
    14161 cgcgcccggg ctcgaggccg gcgaagcgat cgaggaacaa caggtggttg tcctcgttca
    14221 tgagcatctg cccggcgtgc tggccgagga tttccgccat cggcgcggcc cgctcgcgga
    14281 gctgggccac agcggccggg gcgccgtcga tcgtcagctt cgaggtcccc gccgtctccg
    14341 cgatctcgcg gaagacctca caccgggtgc gggtcgcggg atcttcgaac accgcccgga
    14401 tggcggcggt gggggagccg tggcggatga attcgcggtc ggcccggccg cccagggcga
    14461 gggcgagggc ggtgacgatg accgatttgc cggcccccgt ttcgccggtc agggcgatca
    14521 gaccggggcc gaactctagc tcggcgtcgt cgatcagcgc gacattgcgg atggtcagcg
    14581 agcgcagcat cattgcaccc ggcggtgaat ctccgccatc agccgcgcga tcgcctcccg
    14641 gtccccggcc gcttccgcct cggcgagctg ccgtttcagt tcgggaaggc tcgcgcggct
    14701 tctctgcgtg aggaactcct tcagcatctt gcgggcctcg gcctccactt tgtcgggctc
    14761 ccattcgatg gcggcgaccg cagcgatgag cgactgaaag gcctcgtcct gcgcggtgtc
    14821 gaccagtccg cgggcgtcga tctcgccggt cacgcggtac tgccgggcca tggcggcata
    14881 gagccgggtc agttcggggg cctcgaaatc ctccggggcc acgcgttcga aaaccgcgtc
    14941 aatggcgccc gggttatgga gcatgaggga gagcaggtgg agggcgagtg gatcgtattt
    15001 ccgcgcccgc ttggccggcg cggtctcggc ggcagccgcc tcccgcggag ccgccgaaat
    15061 gagcaggtcg ggattgaggc cgagcaccgt ggccgcctcc tcccagaaga ggctgcggcg
    15121 ggtgggatcg gcgatgcgcg ctccgagggc ggcgaattct ttgaccagct tctcccgccc
    15181 gatgatgccc gcgcggcgca cgtcgatatc ctgcgttcga tatgtgatgt atccaaccgc
    15241 ggcgcggcgg agctgctcga ttttctcgcg gccgtgcgcg cgcgccaccg aatcggggtc
    15301 ctcgcccggc ggcgccacca caaccttcac ctccagcccc gcgtcgtaga gcacgtcgac
    15361 cgagcggagg gccgcagccc ggccggccga gtcggcgtcg aaaaagagat agacgctctc
    15421 cgcaaagcgg gccaaaagcc gggcctgcgg caagctgaaa gcggttcccg acgaggccac
    15481 gacgttcttc acgcccgcct gccagaggga gatgacgtcg aagtagccct cgactacgaa
    15541 gacggcgttt tcgctgcgaa tgggatcgcg ggcgaaattg agattgtaca ggacactgcc
    15601 cttggagtag agcgccgtct ccggcgaatt catgtacttg accgtctcgc ccttcttcag
    15661 cgttcgcccg ccgaacgcaa tcggcttctg gctcaggttg aaaatgggga tcatgaggcg
    15721 ctggcgaaaa cggtcgtagt gctggcggga cttgtcggaa tacagggcca gtccgcattc
    15781 gacgagatcc tcgggggaga ggcctttggg acgggcgaaa cggaggaggc tgtcccactc
    15841 ttcgccggcc aggccgagct ggaactcgcg gatggtatcg cgcgtgatgc cgcggcgctt
    15901 catcagatag tcctcgagcg ccgcccggta ccgcggatta aacaactgct tctggaagaa
    15961 ctcgagggcg acgctgttgg cgtaggcgat cttctcgctc gcctcccggc ggccgtcgcg
    16021 ctcgcggtcc tcgcggatgt gaatgttggc tttctgggcg aggaactgta cggcctcgac
    16081 gaacgacatc ccctcgtgct cctggagaaa ggtgaacacc gtcccccccc gctggcagcc
    16141 gaagcagtag tacatctgcc ggtcggggga gacggagaag gagggggttt tctcggtgtg
    16201 gaaggggcag acggcgacca ggcaccgtcc ctggcgcttg aggcggacgt actggccgat
    16261 gagagcgacg atatcggtgg cctggcggac ctgctcgatt gtgtcgggcg caatcatggg
    16321 ctactctttg agcttgacga tggtgacccc ggcgccgcct tcgttccagt tgccgagccg
    16381 cacggaagcg acctccggat gttcccggag atactcggtg atgcgtttgc gcagggcgcc
    16441 ggtccccttg ccgtgcacga catacacctg gtgcagtccg gccacgaccg cccggtcgag
    16501 gtagcgttcg agttcttcga tggcctcgtc gccggtcagg ccgcgcaggt ggatctcggg
    16561 ggactcgacc ggccgggccg acacccgcgc gcccgtcgcc tccggccgcg ccggcgcacc
    16621 gccctcttcg agccggcgga gatggcggat ctcgacggtg gtgaagacat tccgcacctt
    16681 cacccgggcc cggtcgtcgc cgaggagttc gtcaatctcg ccggcctggc cgagggagac
    16741 aatctcgacg cggtcgccct tttcgaaccg gcccggctcc ggcgccgggg tctccgaggc
    16801 tttcttcagc gacgaaagtt ccttggcccg ctcgcgcagg gagcggtgaa agttcttgac
    16861 ctgttgcggc tcggcctgcg agcggcggat atcggccacc agctgctcga cctcgcggcg
    16921 ggtttgctcg atgaaagcct ccgtctcggc gagcgccctg cggcgcgttt cctccacatt
    16981 ggtcgtgagt tcatccacgc ggctcctgta ggtgtcctcg aggcggcggg cctcggcgag
    17041 ccggtcggtc agttcggcgc ggtctttgcg gacgacggcc agctcgctct ccagcgagga
    17101 gatgagcgcg gccagcgatt tttcccccga cccgacgagc cgggaggcgc gctcggtgat
    17161 ctcctcggac atgcccagcc ggctggcgat ttcgatggcg taggacgacc ccggcgtgcc
    17221 gagctggagg cggtaggtcg gggcgagcgt cgtgcggtcg aactcgaacg aggcgttctc
    17281 gatttcaggg ttgtccatcg ggagcgtctt gagttgcgag aagtgggtgg tgacgatcat
    17341 gcgggccccc ttgcggatcg cgtgcaggat gatcgcctcg gccagggcgg ccccctcctt
    17401 ggggtccgtg ccggcgccga tttcatcgaa cagcagcagc gtgcgtggcg aggccttgcg
    17461 gacgccgtcg atgaggttgc ggacgtggga ggagaaggtc gacagggaca gctcgatgga
    17521 ctgctcgtcg ccgatgtcgg cgcagacata ttcgaaaatg ccgaccgagg atttatccgc
    17581 ggcggcaatg tggagccccg attgcgccat gagcacggcc aacccgaccg tcttcaggca
    17641 aattgtcttt ccgcccgtgt tgggcccggt cacgaggatt gcctggcgga ggtcatcgag
    17701 ggccaggctg ttggcgacca cggcctcggg cttgccgaac tggaccgcga ggagcgggtg
    17761 gcgggcatcg acgagactca acgaggactc cggcccgagc gcgggcttgt tcccgccgac
    17821 cgctttcgaa tatttggcgc aggcgtacag actgtcgagc cgtccgatca gccgcgtgtt
    17881 ctccaggagc gcctcgcccc ggcgggcgat ctcggcggtg atggcccgca ggatgcggtc
    17941 gatctccagc cgctcctcct gctgcagcag gttgagccgg ttgttcagtt cgacggtctc
    18001 cggcggttcg atgtacagcg tggcccccgt ctgcgaacgg tcgtggagaa tccccatgtt
    18061 ggcccggtag gagctcgacg gcacggtgat gacgtagcgg ccgttgcgca tcgtcacgat
    18121 gtcgtcctgc acgccggcct ggtgggggcg gtcggcctga atggcggtga ggcgggcgac
    18181 gaggcgccgc ttggcgtcgc cgaattccgc ccgcacctgc ttgagcttgg gcgacgctga
    18241 gtccttcacc tcgccgttct cgtcaatggc gcgccggatc tccgccttca gctcggggaa
    18301 agcgcggacc tgctcgaggt aggcggcgat gagcgggaac ttcgggcgcc cctcccggtc
    18361 gtacccgttc agggcgatcg agacctcgac cagttcgagg acgcggagga tctgctcggg
    18421 atcgaggaat accccttcca cctgggcgcg gagaagttcg gggcggcagt tttccatgcg
    18481 ggcgaggggg aaggccatgc cgaagctgac gatatccctc atctgcgaga tttcgctgcc
    18541 gcggcgctcg atctcagccg ggtcgtacag aggccgtatg cggttgactt catccgcacc
    18601 atagggggtg aggcaccggc cggcgacctg ggcgatcacc ttcggatatt ccagtatgtc
    18661 aagagtatgc tggtcaatca tttcgcgttc tcgacggcgg cggcaggcaa agaaaaagcc
    18721 gaccgtcggt cggcttaaat ataccgctcg gaccgcgaaa gaaaagccaa atcacggctg
    18781 ggcggggtcc gaggtggcaa agaagcggcg catctggtga agcacctcgt agacctcgga
    18841 ccgctgggca tcggtcgtct tcttgttgct cgtctgcgcc gtcagcagag ccgtctctat
    18901 cttggcgatg aaggtgcggt tgcgcgggtg caccggcgcg gtgccttcga ataccagggg
    18961 aacggtcttg gcgaccatcg gcccgaagaa tgactgctca aaggcggtat agaacgaggc
    19021 cggcatgggc aggaggaaca gcaggagtgt gacaaacccg accagcagcc acccgcggag
    19081 gaagccgatg agcgcgccgc ccatctggtc cttcttcccg atcgatttga tgttggcgac
    19141 tttgtagaag accatcccca tcagcttgaa ggcggcgtag gagagagcga tgagcacggc
    19201 aaacgacaga aaagccgcaa ccagaggcga ccccccgact ttctcataga cccagacagc
    19261 gaagcggtcg atgtagttga cggaaacgac aatggcgatc aggaatacca ggaacgccat
    19321 cagctcccgg acgagccctt ttttcgagcc gacgatgacc atcgccagca agagcacgaa
    19381 gagcacaata tcaacccagt tcatccactg cgactcctgt caagaagtgg gtgcgatgta
    19441 acctgggaat agcgtagggg atttttaaat cacttggcaa gcattttcgc ggccagctgg
    19501 ttgaccagct tgccgtcggc ctgcccccgc accttgggca tgagcgcttt catcacgcgc
    19561 ccgaggtcgg ccgccgactg ggccccggtc tcccggatcg cctcggcgat gagggtgcgc
    19621 agatcgtctt cggataattg tctgggaagg tatgcggtga tgaggtcaag ctcccgctgc
    19681 tcctgctcga ccaagtccat gcggttgccg cgctgaaact gctctatcga ctcgcggcgt
    19741 ttcttggcgg cggtggagag gaccgcgatg atatcctcgt gggtcagctc cgcgcccttg
    19801 tcgatcttct tgtacttcag gtccgacttc agaccccgaa ggacggtggc tttgtggcgg
    19861 tcaccggcct tcagggccga agtcagatcc ttgtctatcg tttcaagcag actcatcgat
    19921 gacttagtac ctcgatagtc ttctcaaccg tctctgtttg cgtcgcgcgg cagccatttt
    19981 gcgtttcttg gcttccgacg gcttctcgta gtgctgatgc ttcttgatgt cgctcagtac
    20041 tccggtcttt tcgcaaaact tgttgaagcg gcgaagggct ctctcaaaag actcatcgtc
    20101 tcgaactctg acgcccggca attagataca cctccttttt ggtgcctgtt aaagattacc
    20161 gccggcacaa cggtcggcga gctatccccg catccgggca tagtaccact ctatatcggt
    20221 aagatacaca cacttataca gttgtcaacg acaatcgcag aataaaaggc ccgattcaac
    20281 cggtttgttc cggccaaagg aaacaatcgg aggccgatgt cgatagaaga actacgatgc
    20341 cgtttactgt cgcccatgcc gccgccggcc tgccgctgaa agcggtcctc ccgcgccgac
    20401 tctccctgtc cggcctgatg gccggggcta tggccccgga tctcctgtat ttccttgtga
    20461 tgacgacggt tgaccggggg ctgtcgcact cctggccggg gctgttcctg ttctgcctcc
    20521 cggccgggac agcgttcgcc ctggccttcc acggtttgat caagtacccg ctcctgacca
    20581 atctgccctc cccctgggac cgccgatttt cggggctggc cacggcccgg ttccacccgc
    20641 acggggtgcg tgggtggagt gttgttgtcc tctcggtcgg cataggcgcg ctcacccact
    20701 ttttctggga ctctttcaca caccccgggg gggagctggc gcgggcgttg ccgtttctcc
    20761 aggcgcaggt tcgggcattc ggacaggtga tccccgtgag ccggatcgcc cagcatacga
    20821 gcacactgct tgggatatca gccctcctgt ctgggtcttg gcgggggtgg ctgctgccgg
    20881 cgcccgcgac cggggcgcgc tttcggacag ctcgcgataa atggcgtttc tggctgggca
    20941 gcggtctttt cgcgaccttg ttcgccctgg ccgtcacctg ggcgtaccgg acctactggc
    21001 ccgaccgggt ggccacgaca ttcacgactt tcggcctggc cggctgggcc gggtttttct
    21061 gggtggtggc ggtgtgcggg gcgatctctc gctttcttcc ggggcgaggt cgatcaggcc
    21121 cggccggaga ccgggcccgc cagccagttg gagcgtggcg cagtcaaagc tgagttcttc
    21181 cacttcattg ggcgggcgta tcgcgcggga actcgatttc tcattcgtca gggcgcccgg
    21241 tcgtcttatt cctccacaaa gtgcacccgc tcgcttccct cgttcgagag gaaatggtag
    21301 tggaggtggg gaatctcctg gccgtagccg ttgttgatct ggatgcggaa gtggttttcc
    21361 agcccgagcc ggcgcgccac cagcttggtt ttctcatcga gcatggcgag cacctccggc
    21421 ggagcttcaa agaacgacgg cgtttccgtt ttggggatta tgagcaggtg cacgcggtct
    21481 ttcgggcggt gatcggcgat gacaatgcag tcgtcatcct cgtagaacac cttggcggga
    21541 atttcgcgtc tgatgattct attgaagatt gaggccatcg ttgtcatgat ccttgtaact
    21601 tgttgactgt tagtcggttg ctattgtcgg cgcaacccag cgggcagcgc ggccacccta
    21661 aagtattata cggggcggac ggagcaagcg ccgatcggag cgggggcagg caaatgatgg
    21721 tttggccgat ttccgcctct tgcggagttt gcgggcatca tatattttag taaatgggag
    21781 attatatatg agaatagtaa cgccttaata tacaaggagg atggccatgt caggtcattc
    21841 aaagtgggcg accatcaaac gcaaaaaagg caaactcgac gccgagcgag gcaagatttt
    21901 cactcggcac atcaaagaaa tcgtgaccgc ggcgcgcgag ggaggagggg atccggacgg
    21961 caacccgcgt ctgcgcaccg cgattcaggc cgccaaggcc tcgaacatgc ccgctgacaa
    22021 tatcaagcga gccatcatga aaggcaccgg cgaactgccg ggcgtgagct acgagtcggt
    22081 ggtgtacgag gggtatggtc cggcgggggt agcgatttat ctcgaagtcc tcaccgacaa
    22141 ccgcaaccgc acggtcagcg agattcgcca cttcctcacc aagtacggcg ggaacctggg
    22201 cgagaacggc tgcgtcggtt ggatgttcca taagaagggc gtgatcgaga tcgacaagac
    22261 cgcggccgat gaggacaccg tcatggaaat cgcgctggaa aacggggccg aggacgtgcg
    22321 caccgacagc ggctcgttcg aggtggtcac gcccgcggag gcgctcgaga ccgtccgcgc
    22381 cgccttcgaa aagaagggag tcccgatggc gtcggtcgag gtgaccatga ttcctgaaac
    22441 cacggtgaga ctggagaaag agggagaggc gtcgtcgatg ctgaagctct acgagctgtt
    22501 ggaggagcac gacgatattc agaaagtgta ctcgaacttc gatatcgacg agaggctgat
    22561 ggagcagctg gcgtagccgg gcggagagga gagtttgcag cgcgccgggc agcaggccga
    22621 agagggtctg cggcccggcg cgttcttttg tgcgtcggcg ccgccaggcc gccacgatcc
    22681 cgggagcacg ggcacagtgg gggatctgcg acagcgcggg agggagattg tcgccgcgcc
    22741 gcgacagcgc ccggaggcgg gtcgccggat cggccgacgc agccgcgcac agcggaccat
    22801 gcgacgtctc gacggtccgg aagaaccgcc gacctatttc tgcggcattc acgagcctga
    22861 gcggccgccc ggacgccggc cgcgggaagc ggcgagctct tccatgcgca gacttccccc
    22921 ggtgcgtgcg atccggcaca atcgttgctt tggcggaagg cgaaagaggc aaccaaacgg
    22981 ccggcgctgg tccggctgtg ccgacaacac taactccggt ttgggaggtt ctgatgaaga
    23041 catactcgtg caatgcaact cgccgcgcgg cagcgaactc cccaccgacc tctcgagcgt
    23101 cggcgagagg tacgttttgg aggctgactc cagcgggctg tgttcgagca aggacggctt
    23161 gctccatgtc gacttcacgt tctccacgaa gccgaattcc ctcaccttca cccaggggat
    23221 tgcgcacccc acgtactggc actcggtgac cacgagcact ctcacgctca agactctcga
    23281 cgggacgggg tatttcgcgg tgcagtttct cctcgcccgt cagtagcgac ggggctcttc
    23341 ggcggactcc ggcacaggcc ggggtccgcg cggcgacgag ggccgggtgg aaccgatctt
    23401 gacggcgccg gtcgaatcgg cgtaccttag cctcgctgtg acaagaggaa gactgcaagg
    23461 aatcagcggc atcctgtgcg atatcgatgg cgtcctttgg gacagccagg atcgcaagag
    23521 gccgacgccg ggggcgcacg aggctatccg ctgcatcaag gaagaacggc gtctcccggt
    23581 gcgcttcgtc accaacacca ccacccgcag ccgcgacacg ctctaccggg aaatccgcca
    23641 gcggggctac ccgattgagc gggacgagat tgtcgcgccg accacgctgg gtgccgagtg
    23701 gcttcgcagg aaaggacgcc ccagcgtctt cctggtcatg cgcgaggaca cgcaggagga
    23761 attcgccgag ttcccgcgcg acgatgtgcg gcccgactac atcgtgatcg gcaacaaccg
    23821 cgaccggtgg gactatgccc tggtgaaccg gatttttctc atgctcgtca acggctccga
    23881 gatgctcgcc ctgcacaagc agcgttactg gcagagcggg gacgacatcg cggtcgatat
    23941 cggctgtttc gtgaccgggc tggaatactc cagcgggaag cgcgcgaccg cgatcggcaa
    24001 gcccgagccg ctatttttcc aggcggcgct cgaggcgatc ggggtggcgc cggcggaagc
    24061 gatcatggtc ggtgatgata tcgagacgga catcgggggg gcgcagcggg ccgggctgcg
    24121 gggtgtgctt gtgcgcacgg gcaaattccg cgagtacctc gtgcggcagt caacggtggc
    24181 cccggacctg gtgattgcct cgctggggga cctgccatcg catctgtaac ggggtggtgg
    24241 ctgccgcgca taagccgaat gtgacttggg cacggccaga tttaagttga attcgggccg
    24301 gctttgcctt acttgagcct atgcgcgtgc taggcatcga tcccggactg cagatcaccg
    24361 ggtacggggt gatcgaggac ggcgggggag cgccggtggt gctcgaagcc ggtgtgatcc
    24421 ggacgacgag cgggcatgat atgtccttgc gcctgcatga gttgtcgtgc gagattaaag
    24481 gggtgatcga gcagttccgg ccggatatca tcgctgttga ggagctgtat tcgcactacg
    24541 gtcacccgcg cacggcgatt atcatggggc acgcccgagg aatcgtgttc ctcaaagcgg
    24601 ccgaggccgg ggtcgcggtg gtcccgtacg cctcgacgcg catcaagaag tcgctgacgg
    24661 gcaacggccg ggcgagcaaa aggcaaatgc agatgatgat ccgttcggcc ctgcaccttc
    24721 cccgcccacc ggaacccccc gatgcggccg atgcgctggc ggtggcgctg tgccactgcc
    24781 gggcggccgc gcaccacgag gtggccgtat gatcagccgc atctccgggc ggctcgtgaa
    24841 catcgagaac gacaccgccc tggtggagac ggggggcctg gcctacgaaa tcatgctgcc
    24901 gtcggccctg gccgagcggc tgcgcgacga cgggaaagtc ggcggggaca tcagtctcga
    24961 gacgctgtac tatatcgatg ccggggagcg gaagtcggcc catttccccc gcctggtcgg
    25021 tttcgtcaac ccgatcgacc gggagttttt ctcggttttg acccatgtat ccggtttggg
    25081 ggtaaaaaag gttctgaagt cgctggtcat gccgatccgc gacattgcga cggcgattga
    25141 gaacaaagac gcggcccaac tcaaccggct gccggggatc ggcgcgcggc tggcgaacaa
    25201 gattatcgcc gaactgcacg gcaaggtggc gaagtttgcg ctctccaagg aggaccaggc
    25261 gctctccaaa gcggcggaac cggtcgagcc gctcggcgag gaggcccgcg cggtcctgct
    25321 ccaactgggg tactcgcgga gcgaggccga ccggatggtg gaaggggcgc tcaagcacgg
    25381 tctgaaggcg gcgcgggtcg aggatttgat cacggtgatt ttccgcaacg aacagcagaa
    25441 gaaggcggct gagtgatggc gcgcgagcgg atcgtgtcag gcgagcagat tattcccgac
    25501 gaggacaagg cgctggtgac gctgcggccg agccgcttgt cggagtatat aggacagaat
    25561 aagttacgaa acaaacttaa ggtcacgctt gatgccgccc ggggtcgggg ggaagcggtc
    25621 gaacacatgc tgttctatgg gccgccgggg ttgggcaaga ctacgctggc gtacattatc
    25681 gccaacgaga tggggaccaa actggtggcg accgccggcc cggcgctcca gcgggccggc
    25741 gacctcatgg ggattctgac caatctcaac gagggggata tcctgttcat cgacgaaatc
    25801 caccgcctca ccccggtgat cgaggagttc atctatccgg cgatggagga tttcaaggtc
    25861 gatttcgtgg tggacaaggg ggcgttcgcc aaggtcatca acgtgccgct gaaaccgttc
    25921 acgctgatcg gggcgacgac gcgggcgggg tttctgtcgg cgcccctgcg cgaccggttc
    25981 gggctgtact accatctcga tttctacccg ccggaggagc tggccgacat cgtcctgcgc
    26041 tcagcccgcc tcctggaggt ggagattgag acggaggcgg cggcgacgat cgcccgccgg
    26101 gcgcggggca cgccgcgggt cgccaatcgg ctcctgcggc gcgtacgcga cttcgccgag
    26161 gtcaaggcgg cgggggtaat cacgcccgag gtggcggccg cggcattgga cgccgagggg
    26221 gtcgattcgc tcggactgga tgcgctggac cggaatctga tccgcgtgat tatcgaattc
    26281 taccacgggg ggccggtcgg tatcgaggcg ctgggggcga ccctgaacga agagatcaac
    26341 acgctggtcg atatggtgga accgtacctg ctgaaaatcg ggtttgtgcg gcgtacgcgc
    26401 caggggcgag tggcctccga ggccgccgcg aaacacctcg gcgtcaccct gccccgcacc
    26461 ggtcgccagg gaaggctgct gtagaaaaga acacgatgca atccgaaccc tggatcgaag
    26521 tcgaccgcta tctgaccgct ctccacgttc cccccgatcc cgtgctggag tcggccctgg
    26581 cggccagccg ggaggcgggc cttccgtcaa tccaggtcac gcccctgcag gggcaattgc
    26641 tgcaggtgct cgcgctggcg tgcggggccc ggcggatcct cgagatcggc actctcggcg
    26701 gttacagcac catctggctg gcccgggccc ttccgcccgg cgggcggctg gtcacgctcg
    26761 aagtcaactc ttcgcacgcc gaggttgccc gcgccaacat cgcgcgggcg ggactcgccg
    26821 ggacggtcga aatccgggtc ggacccgcgc tcgcctcgct ggaggcgatc gccgcgaggg
    26881 gggaggggcc gttcgacctg acctttatcg acgccgacaa ggagggcacc ccggagtatt
    26941 tccgccgggc gctcgacctg tcccgggtcg ggggtttcat cgtggtcgac aatgctgtgc
    27001 gccatgggaa agtggtggag cgcgacagcg atgacccgcg ggtggccggc atgcggcgct
    27061 ttctggaatt ggcagccgcc gaaccgcgcg tcaaggcggc gacggtccag acggtcggcg
    27121 cgaagggcta cgacggcttt acgctcgcgc tggtgacggc ggagaggcgc ggagggggcg
    27181 ggaatggatg acgatatccg gctgctgctc gaggcgctgg gc
//
LOCUS       DAOPOE010000039        27101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2272_length_27101_cov_5.938142, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000039 DAOPOE010000000
VERSION     DAOPOE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27101
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_27101_cov_5.938142"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1223
                     /locus_tag="PKM94_06910"
     CDS             228..1223
                     /locus_tag="PKM94_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOD66408.1"
                     /translation="MPEYKQIDRVIRILQRLYQQGHVTVRELYELFERQVPKRTLQRD
                     LVDISGAGVPLYNEKGRGREQIWCVDKHFLRFVPQTIGSQELLASFFLEQLALAARGT
                     QLEEDVRSLLDKARQLAGPGVFQSLQSGDLAHDMFGATFMGYVDYSEHSEKIDTLVKA
                     CAESRTCRFVYKAATREKPSEFEAEPYLMLYHKGAMYAVVYVPAHDNYIFLPVQRIRS
                     VEYTGERFTRQAGFSLDKLREGRFGIYGGPEYRPERVVLKFKPEIADVVAERVWHYSQ
                     QMTRNADGSLTLEMCTVISDELRAWIGSWLDRVEVLEPQGLVPQAGSGEHPEAAH"
     gene            complement(1282..3129)
                     /locus_tag="PKM94_06915"
     CDS             complement(1282..3129)
                     /locus_tag="PKM94_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOD66409.1"
                     /translation="MSRRLLFALAALLLLGIAASAAGREDESLPIGLTEEEKGRLDDI
                     GKAHRSTYPPTGSLRNPAEWERSVGVIIRWPLGISYALVAEMSEELMVTTIVSSTTVE
                     AQARAAYQANGVNMANVNFLIAATNSIWTRDYGPWFIFSGDRIGIVDHIYNRPRPLDD
                     VIPQAIGSAWGIPVYGMDLIHTGGNHMSDGLGRSMSTRLVYDENPSLTSAQVDSIMLA
                     YLGNQYTVLGYVESSGIHHIDCWAKFLDPATIIVKDVPPSDPSYSLLNARAAYLSQQM
                     SAWGRPYEIIRVYCPAGTAYTNSIILNGKVLVPIFGSPSDAVAIQTYQDAMPGYEILG
                     FTGSWLDDDAIHCRAMGVPDRQMLFIDHDPLHGRVRSGGNGQTVSAFIDDYSRAGLIA
                     ESLLVRYRVDGGVWSDAPLAPAGSFYDGLIPAQPGGSAVEYFILAADNSGRVEQHPFI
                     GAPGAHAYSVNFPPAIISPDSFLCRVGDRFAYSPGLSDSDDTAHAVWFEGYPAWLQPA
                     GDSLAGTPAERDTATFSVFAADPFDTTGADVFVRTYICGDVDDNGAGPNVADLTYLVT
                     FLFRGGPEPPVLPAANLNGSTGPGNNIEVSDLTLLIAYLFRGGPAPTCG"
     gene            3506..4741
                     /locus_tag="PKM94_06920"
     CDS             3506..4741
                     /locus_tag="PKM94_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOD66410.1"
                     /translation="MGNPDTFLTRRRFIETSLGCAVTAGLATLVPGRSFGQAEPPATQ
                     PSTPAPGSIIHRTLGRTGFTLPIVNMGVMNNNSPEVVRASYELGVRYFDTAARYQNGR
                     NEEMVGAVIRQLGVRDKVVISTKEILPGRREGDGAGAVKRKLVEQCEASLKRLGMETV
                     DILYLHGVSSPEEMKEAGALEALAELKKQGKVPAVGVSTHQNMAAVLKAAAEGGFYDV
                     VLTVINVSLADDADLLGAIAAAARAGVGVVAMKTQAGGARLPNPATLKDYAGGTIATA
                     SLKWVLRNEHIASAIPGYDNFQHMEEDFSVARGLDYTAEEAKFLSDNRLTLGLAFCRQ
                     CRRCEPSCPHRADIPALMRTHMYAAQYANFEEARATLGEIAPGRGLHACGACERCAAR
                     CAHAVDIPGRIERLKTIYA"
     gene            complement(4754..5641)
                     /locus_tag="PKM94_06925"
     CDS             complement(4754..5641)
                     /locus_tag="PKM94_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66411.1"
                     /translation="MKHSFAGAAAAAALLALSVAGCGGKESAGTAGGPAQFLRTRLGA
                     AEPTGEVRTFTGETLWEYIDGDAEMYRQYRFVEIATADYRFPEVEFIVDVYRFADDAG
                     AYGLYAAVRPDEPDLLAIGTQGFCTESTVTAVKGRYLARLIAFDESSQTGAMLAVAAD
                     SLMRRLPGSTDRPAAFARFPDSARVPCGDRYYPASYLGQAFLTDVYEQAYALGPDTVR
                     LFFAADSSGGKFLRWQEAAGAEKVQTDLPFDGGAALMIRSYADTAAAGLRQFTLAGMR
                     AYAPRHRDFFARWLAALGG"
     gene            complement(5691..6248)
                     /locus_tag="PKM94_06930"
     CDS             complement(5691..6248)
                     /locus_tag="PKM94_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD66412.1"
                     /translation="MDQHRADSADLHRMRRADRARDEAWISDLVARAPFCHIATVAGD
                     QPFITPMSFAYDPDRQALYFHTAGAGRLRTNTERGSRVCVSFAEMGRLLPAKTARGFS
                     VEYRSVIAFGRISLVDDPAAAARHMQMLIDRYFPRLRPGVDYRPIQPEEIEEISVYRI
                     DIDAWSGKEKSAPDEFPGAFSWESR"
     gene            6408..7067
                     /gene="rpe"
                     /locus_tag="PKM94_06935"
     CDS             6408..7067
                     /gene="rpe"
                     /locus_tag="PKM94_06935"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOD66413.1"
                     /translation="MIEIAPSVLAADFSRLGAEIADTAAAGADRLHLDIMDGHFVPNI
                     SYGPDIVRTVNRLTGLPLDVHLMLTNPEAFFEPFAAAGADAITFHIEVHPDPVAHLKR
                     LEEMKVARGLSLNPDRTAESVLPFLELCDQLLVMSVFPGFGGQRFIEETLRTVEAARR
                     YIDRHRLRTQIAIDGGIDGNNAQRAVDAGADLLIMGSAFYRSTDRAGLVRKVHGLKRA
                     G"
     gene            7417..8619
                     /locus_tag="PKM94_06940"
     CDS             7417..8619
                     /locus_tag="PKM94_06940"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOD66414.1"
                     /translation="MANVKTKKGVIGILTGGGDVPGLNPAIRAVTIRAIREGYRVIGL
                     RHGWAGTIDIVRDKKHDNSGAFVELTEEIVNKAGRTGGTFLHSSRTHPGHVTQENIPP
                     HLRDKYKGAKSDLTPEVIKNLDWLGIDYLIPIGGDDTLSYAVRLYKEGVKIVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTIMMTNLLRTSAGSHERFLVLEVFGRYAGFTAMLPTMAG
                     AANRCVIPEHQFNIDLLAELLTYDRNHNPSRYAVVLVSEGAMFEGGEMVFQDSAADAY
                     GHKKLGGIGDLVSAELLVRSPKYNNGKQIRCITQKLGYLVRGGDPDAVDSIVPMAYGN
                     LALDLILKNIHGRLVVLKNGRYDDVPVDVVSSTKKLVDVERYYNRERLRPFYHSFEMQ
                     PLFIMTSE"
     gene            9019..10305
                     /locus_tag="PKM94_06945"
     CDS             9019..10305
                     /locus_tag="PKM94_06945"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOD66415.1"
                     /translation="MSKGSFNPFLMAQTQFDGVADILELDKATRELLRNPLREYQFSI
                     PVRMDDGAVQVFRGFRVQHNDSRGPCKGGIRFHPMETIDTVRALAMWMTWKCAVVDIP
                     LGGGKGGVVCDPHNLSQREQEAICRGWVRQLAKNVGPIADVPAPDVMTSPQHMLWMLD
                     EFEHIHGGKYPGFITGKPVGMGGSLGRTEATGFGVVFTLREALKQLHIQPDKTAASVQ
                     GFGNVAQYAIKLYQQLGGTVIAVSSWDQADQTSYTFRRTSGIDLQQLLGITDRFGGID
                     KGKAAGLGYEVLPGEAWLEQEVDVLIPAALENQISGENVEKISRRVKVIAEGANGPTT
                     PEADKVISARNIFVIPDFLANAGGVTCSYFEQVQSNMNYYWPKEEVLQKLDQKMTAAF
                     LAVSELASRKKLYMRDAAYVIAVGRVAQACRDRGWV"
     gene            10480..13701
                     /locus_tag="PKM94_06950"
     CDS             10480..13701
                     /locus_tag="PKM94_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD66416.1"
                     /translation="MTDEQKPIGDYWVLDRLIGSLQERAKELNCIYRVGAILTNPDEP
                     IGRVCPLLIEAIPTGWQYPKLCQVKIVIEDNIFATPNFIETPWLQSADIAVQDIVIGS
                     ITVSYREETPSAAEKLFLADESRLLKTIAERLGYFICHQKMRQITEEWQTVRPEVSVG
                     RPEWQVVLKLLRDTDRELYQTIARKMLNHLCWSGIAEAEKLLRSFTSNESLEDEENPE
                     HWNRPHQRKSLVMSAAFSSAAFKIAADHLDAGEILNLIQKWIQEDKLGFLVQAVNRNL
                     TLSEVADAIRRYQHLTQDEPVTLSSSMRGIQVSLIRRFLSDQQQYINVAKNYVDVADF
                     CKLIQNLIYTSESYGRLGGKSAGFFLAKQILLKKAADNELLANVQIPKTWYITSDVIL
                     YYMHYNNMDDVVEQKYKDIGQIRREYPHVVQSFKNARFPADIIKGLSMALDDLGDRPL
                     IVRSSSLLEDRVGAAFSGKYKSLFLANRGSKQQRLDALTDAIAEVYASTFGPDPIEYR
                     AERGLIDFAEEMGIIIQEVVGRRIGKYFLPSFAGVAFSNNEFRWSPRIRRTDGLIRLV
                     PGLGTRAVDRLSNDYPILIAPGQPTLRTNVTPDEIARYSPKYIDVINLETEDFETIPL
                     LDFLREVNFEVPGIQHMLSLSMDGRLVAPGLTADISSGAPVVTFDGLVSRTRFLKRLR
                     AIMQTLEVTLGEPVDIEFACDGDDFYLLQCRAQSYSAESAPAPIPKDIPMQQIVFSAN
                     RYVSNGRVPDVTHVVYVDPRKYAEIEDRATLVAVGRAVSRLNKLLPKRKFILIGPGRW
                     GSRGDIKLGVNVTYSDINNTSMLIEVAIRHGNYVPDLSFGTHFFQDLVEANIRYLPLF
                     PGDDHVVFSERFLTEAPSVLPELLPECDELAEVIRVIDIPQATGGKVLSVLMNADLDE
                     AVGILTPPGGGLEIAASPAESPAQVRGDFWRWRLQMAEQIAARIDPVRFGVAALYLIG
                     SAKNANAGPDSDIDLLVHFRGSPEQRQDLVSWLEGWSLSLDEVNFIRTGYRTGGLVDV
                     HMVTDDEVSAKSGYAARIDAVTDPARPLAMAQRQPAGKGTAGH"
     gene            complement(13750..15246)
                     /gene="amrB"
                     /locus_tag="PKM94_06955"
     CDS             complement(13750..15246)
                     /gene="amrB"
                     /locus_tag="PKM94_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD66417.1"
                     /translation="MTRFTVCFLLVLPAAAALAASAPPVTRPPAVAGSFYPGDSAELA
                     AMIAGQLAAAPAAAIDGRIVAIVVPHAGLVYSGPIAAHAYKALAGRPIETAILCGPAH
                     RYGFRGASVYGPGVTWQTPLGPVPCDDARIERLLGASSWLKVVPEAHLKEHGLEVQLP
                     FLQTVLPGAHIVPIAMGYPDGHTVDGLTAALVAAARPPGAVLIASTDWQHYRPAAGGY
                     PMDSLGLACLAAFDADALLRNLEAKKVEACGGAGVVAVMRAARKLGADKVKILGYGDS
                     GDLTGDKSSVVGYAAVAFYCSADGENSAAPGEAAAPEKGMEPYDLTQEERQTLLTIAR
                     RSIIGYLEDGAAPDFEAAGKLAEPGAAFVTLEKQGRLRGCIGHVVAADPLFRTVAACA
                     VQAAVGDPRFEPVTRSEIDQLTIEISVLTPLQAVTIFEEIEVGRDGLIISLGPNRGLL
                     LPQVATEYGWTREEFLRQTCRKAGLSPDAYKSPQAKVERFQALVFGEE"
     gene            15330..16247
                     /locus_tag="PKM94_06960"
     CDS             15330..16247
                     /locus_tag="PKM94_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD66418.1"
                     /translation="MQRVVVVKSVSFSGGRRPGPETYRRLLEVGLAHLAGLPPAAALR
                     SLLPGGTIGMKVNALVGPRNSTPPALTAAAGDLLEAAGWAAGDLIVWDRSNRELDRAG
                     FTLNVGGNGRRCFGTDTAGFGYTAEIHTYGEVGSRVSRVLTEAVDSNINLPVLKDHSI
                     VGLSGGLKNMYGAIHNPNKYHDHCGDPYCAHVSLLEPVRRKNRLTIADAVLVQYHGGP
                     GFREEHVAPYGAVLLSDDPVAVDRVGLEIIEYLRARAGLGTLAEDKRAVTYLDTAACG
                     LGAADLARIDVIVRAVDGAGRETSGELFS"
     gene            16244..17386
                     /gene="amrS"
                     /locus_tag="PKM94_06965"
     CDS             16244..17386
                     /gene="amrS"
                     /locus_tag="PKM94_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD66419.1"
                     /translation="MNRRSFLRCLGCGAAATALPHADFLPDLLGGIENACAIDLSDAL
                     SSEEARHYTKLAGGGIECRLCPRRCRVTDLERGYCGVRENRGDTYHTLVYGKPCAVNV
                     DPIEKKPLNHFYPGTAAFSLATAGCNVNCKFCQNWEISQSRPEQTKNFDLPPEAAVAL
                     CRQQGAPTIAFTYSEPTVFYEYMYDIARLGRAQGIRSVMITGGYIEEAPLAELMPHLD
                     AIKVDLKAIRASYYRDVVRGELTPVLERLQQIRKAGLWLELVYLVVPTLNDSDAEFRE
                     LGQWVFRYLGADVPLHFSRFTPQYLLEHLPPTPVATLERAHAIAGAEGLRFVYVGNVP
                     GHRAESTWCPGCGALVIERRGYRTSAAGLAGDRCVACGREIPGRFK"
     gene            17383..19533
                     /locus_tag="PKM94_06970"
     CDS             17383..19533
                     /locus_tag="PKM94_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66420.1"
                     /translation="MNRSAFLLGFVATGGQTLLLRELVSSLGGSELFIGSALFGWLAA
                     VAAGAWAGGRWPGRLRAALLLVLASLVLPLCVAAARLAPLAYGVAVGEAIPLVPALTI
                     SFLVMAPLGVLSGWLFPAVAGEHGAPADGIVRVYLFEGIGAFAAGLAVVLAAGTVLST
                     PAVALALVVIVMAAALGGGASMRTVRGAGAVLAAVSLLAAVAALDHPLNRALEAAKYA
                     GYAVAASFDTPYSHQALLLRDGAWVLLTDNAVEATRPDAAAAESHLLPVLAYRPGARE
                     VLFIGRTEFGVSALARQLPDLTVTSVDPRARLTAALAEHGLAGEGDPPVHELPRRFIA
                     GGTGRGEFDAIVLDPGDPSTYRAAGLLTPQFLGQLRRVLNDSGVVLLATAYDTDRFVG
                     RREAELLATLRASFAQVFAHVILWPGEQTLLLASDAAVFDVPLDTVFARLDSLPCRPL
                     WVNSSYLFDRLSPLRTERLEAALAGQGAPTTIDRPALTLEHIGFRAERHPVDAAIVGL
                     TGSPAWWAVGVPAALVGLIVWGAAGRRAGRRYGLVLYFVAGAVSLAAEQLAFYVYQSR
                     AGSLYSEIALLIGAFMLGLAVGTWYAARQPQQRGLIWPSLSTLALALAIYLTLTGRID
                     PSAFLLYHMLFLFVAAAATGSLFVAATRRCYPSAAEANRGLGYACELAGSSLGALLVP
                     TVLLPLLGVPAILIGLIGLVGLAGVGAAIVDRRT"
     gene            19612..21366
                     /locus_tag="PKM94_06975"
     CDS             19612..21366
                     /locus_tag="PKM94_06975"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOD66421.1"
                     /translation="MREELRTGCMDIQRGRGRDDAARLRRLLEAAWDWAMADRPEWAT
                     STGYPKYHDRWTDCSEGAFARRREATALLIETLAAIDREKLSARDRLSYDLFLWRQEQ
                     DRARQRFPEELLGLNQMDGVQQDAARIVGLMPVETAAQRDAVLARLEGLPLLVEQTIG
                     LLAQGLARGITPPREPLREVPRQVLNQIPAAAAEAPLLSVLRRLPTALSGAARERFCA
                     QATALFAERVAPAFRGLHRFLEGSYLPGCRETVSWRDLPGGDDWYRTLIRYYTTSELT
                     PDAVFETGMAEVGRIRGEMERIIAETGFAGDFAAFCEFLRTDPRFFCTSAEQLLSTYR
                     DIAKRIDPELARLFGRLPQLPYGVIPIPPYAEKSQTTAYYQPGSLRAGRAGYFYANTY
                     NLASRPTWEMEALTLHEAVPGHHLHIALMQEMEDLPEFRRYSWLEAYGEGWALYAESL
                     GEEMGFYRDPYAEFGRLTYEMWRAIRLVVDPGLHARGWTRQQAIKFFRANSSKADHDI
                     AVEIDRYIVWPGQAVSYKTGEMKIKALRRRAEERLGERFDIRAFHDELLAHGCLPLTV
                     LEERMAGWLAGQEAPAAR"
     gene            complement(21373..22023)
                     /locus_tag="PKM94_06980"
     CDS             complement(21373..22023)
                     /locus_tag="PKM94_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOD66422.1"
                     /translation="MKEFVVIGLGNFGATVARELTRLHCRVTAVDANEARVRDLQDIV
                     HVAIVADAAERRFLEHLEVENFDAFIVSTGVDSHASILITLHLKELRARQVIVKANSA
                     DHAKILIKVGADDAIIPEEQMASRLSHSLAQSNLLDYLPLSAEHFVAELVPPDSFVGK
                     SLRDIQLRARFNIQVIATKHARTGDYIFAPGGDYVIRDHDLLIILGKEEDIDRLRQ"
     gene            complement(22074..23423)
                     /locus_tag="PKM94_06985"
     CDS             complement(22074..23423)
                     /locus_tag="PKM94_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOD66423.1"
                     /translation="MEFLGADPGRKAILYFLAAILLGAFVLSLPLSHGARPVGVIDAL
                     FTATSAVCVTGLTVVDTGTAFSPFGQVVILVLIQLGGLGIMTFATLLIAAAGAQVAFR
                     DRLGVAGSLAAGTFMRTKSLLQAVIGVTLTIELIGALVLFLLFSGSLPLGQAAWHAVF
                     HSVSAFCNAGFSTFSANLEACADRPAILLTVAALIILGGLGFAVITELLHRASGQSRR
                     LSLHTRICLSATAVLLVGGTLAVFFAEYGNTLAGFSPAGKAVNAFFQAVVCRTAGFNS
                     VSQARLTEVAVLVSMVLMFIGACPGSTAGGIKTTTITVIVTTAWDRFRGRRTPSIFHR
                     RIGEESIIRAVGVFILAVFVVAAMLALLAVLWDRPPLRAGSVHGWLAENLFEVVSAFG
                     TVGLSLGITPELNMPGKIVIILTMFIGRVGLLTLAFGLARPTATGEIVYVEEPIAIG"
     gene            23528..24922
                     /gene="tilS"
                     /locus_tag="PKM94_06990"
     CDS             23528..24922
                     /gene="tilS"
                     /locus_tag="PKM94_06990"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOD66424.1"
                     /translation="MLNKVRQAIRDGHLLAPGDAVLVGLSGGPDSVALLHALWALRKE
                     LGLALTAVYINHGIRPAAAKREERFCAALCRQLAVELTIVREDIPMLAGRERKGLEET
                     ARDYRYRIYERLADERGCRRIALGHQANDQAETVLFRVLRGTGRRGLAGIPRKRGRVI
                     RPLLDVWRDEILGYLKEHRLTFCRDESNRDLRFRRNFLRHKVMPLVRKSFNPKVERAL
                     ADLADTLAAEEEYLEGVLAGVQKKVLTETPGGKFELDLRRFDGYDKWIRRRLLRYCLS
                     KISSDGTSPDKEVVERLDRAALAGRAMLSLPGGARAERIGAVLVLGRRVGAFRAELTP
                     GVPAKLERPRLNITARIRRGGSSAIRPVRRGRKVYLDWEKVVPPLEVRSIRPGDRFRP
                     LGLGGRKKVGDFLTDRKVPRLRRDEIPVVADRHGIVWLVGWEIAERVKIDSATRKVLT
                     IECSERLPGQAETV"
     gene            24879..25427
                     /gene="hpt"
                     /locus_tag="PKM94_06995"
     CDS             24879..25427
                     /gene="hpt"
                     /locus_tag="PKM94_06995"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOD66425.1"
                     /translation="MSAARDYQAKPKPFELLLAQDRIARRINELGQAITRDYAGRTPV
                     FLGVLKGCVVFMADLMRAVKLPLEVEFISAESYHDGTAQQELKLDHWFTRSLRGRDVL
                     IVEGVVDSGRTVEAISRELAAVEPASVEVVTLIDKPANHRRKLVIKYVGFALGNEFVI
                     GFGLDNTQRYRNLPFIGRMIEE"
     gene            25626..>27101
                     /gene="ftsH"
                     /locus_tag="PKM94_07000"
     CDS             25626..>27101
                     /gene="ftsH"
                     /locus_tag="PKM94_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD66426.1"
                     /translation="MFWIALALAVVVIYSYMNSFSGDQAEVTYSEFMAEVESGNVAEV
                     TFKDREVQGSLREPKAFESIQRGQTVTKFKTRIPFPDYNYGLVDRLQKQNVKIIAKTE
                     SPLLGYLLAAAPWLLIVLVWLFFIRQMQGASGPRGLFSFGKSKAKLLTDERPKVTFKD
                     VAGVVEAKEELSEIIDFLREPGKFQRLGGKIPKGALLLGPPGTGKTLLARAIAGEAGV
                     PFFSMSGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQIVVPNPDVKGR
                     EGILRVHARKVKLSEEVDFEVLARGTPGMSGADLANVVNEAALLASRKNRDAVTMEDF
                     EEAKDKVLMGSARRSLVISDEEKKVIAYHESGHTLVAKYLPKADPIHKVTIVPRGMAL
                     GVTQSLPVDERHTHSKEYLESTLAVLMGGRVAEMIVFNQLDT"
BASE COUNT         4590 a         8401 c         9664 g         4446 t
ORIGIN      
        1 cactttcgca attcgcggtc ggtcgctgcc tccccgatca gcgcctcgac cgctcagacc
       61 cccgcttgcc gcaccgcacc cccgcgatga cccccgatca cggggtgcga actgcggccc
      121 gcacgacgtc tacccataat tagtgttgcc atgcgtcatt cggtgactca tactctgttt
      181 aatttcggga aaacatgagc acgagagctc gctaatggag ggtttagatg cctgagtaca
      241 agcagatcga ccgggtcatc cgcattcttc aaaggcttta ccagcaaggg cacgtcaccg
      301 tcagagaact ctacgagctg tttgagcgcc aggtcccgaa gcggacgctg cagcgcgacc
      361 tcgttgatat ttcgggggcg ggggttccgc tgtacaacga aaaggggcgg ggccgcgagc
      421 agatctggtg tgtcgacaag cactttctgc gttttgttcc gcagacgatc ggcagtcagg
      481 aactgctggc gtcgtttttc ctggagcaac tggctctcgc agcgcggggg acacagctgg
      541 aagaggatgt ccgctcgctc ctggacaagg cgcggcaatt ggccgggccg ggagtctttc
      601 agtccctcca gagcggggat ctggcgcacg acatgttcgg ggcgacgttc atgggttatg
      661 tcgactacag cgagcactcc gagaagatcg acacgctcgt caaggcctgc gccgaatcgc
      721 gcacctgccg atttgtctac aaggcggcga cgcgggagaa accctccgaa ttcgaggccg
      781 aaccgtatct gatgctctac cacaaggggg cgatgtacgc ggtggtgtac gtgccggccc
      841 acgacaacta catcttcctg ccggtgcagc ggattcgctc ggtcgaatac acgggggaga
      901 ggttcacgcg ccaggccggg ttcagcctgg acaaactgcg cgaggggcga ttcggcatct
      961 acggcggacc cgagtaccgg ccggagcggg tggtgctgaa gttcaagccg gagatcgccg
     1021 acgtggtcgc cgagcgcgtg tggcactata gccagcagat gacgcgcaac gccgacggtt
     1081 cgctgacgct ggaaatgtgc acggttatct ccgacgaact tcgggcctgg atcggcagct
     1141 ggctcgaccg cgtggaagtg ctggagccgc agggtctggt gccgcaggcc ggctccgggg
     1201 agcatccgga ggcggcgcac tgaggccgcg gcggggggca cgctgccggg tgttcgatcc
     1261 gggcagggat ccgcgggttg ttcacccgca ggtcggggcc gggccgcctc ggaacaggta
     1321 ggcgatcagc agggtcaggt cggacacctc gatgttgttg ccggggccgg tgctgccgtt
     1381 caggttcgcc gccggcagca cgggcggttc gggcccgccg cggaacagaa acgtgaccag
     1441 ataagtcagg tcggcgacat tcggtcccgc gccgttgtcg tcgacgtcgc cacagatgta
     1501 ggtccggacg aaaacgtcgg cgccggttgt gtcgaacgga tccgcggcga agaccgagaa
     1561 cgtcgccgta tcgcgctcgg cgggagtccc cgcgagcgaa tcgccggccg gctgcagcca
     1621 ggccgggtat ccctcaaacc acaccgcatg ggccgtatcg tcgctgtcgg agagccccgg
     1681 cgaatacgcg aatcgatcgc cgaccctgca gagaaacgaa tcgggcgaga tgatagcggg
     1741 ggggaagttc acgctgtagg catgagcgcc cggggcgccg atgaaggggt gctgctcgac
     1801 ccggccggag ttgtcggcgg cgagaatgaa gtattcgacc gcgctgccgc ccggctgcgc
     1861 cgggatcagg ccgtcgtaga aactgccggc gggcgccagc ggggcgtcgc tccacacgcc
     1921 gccgtcgacc cgataccgga ccaggagcga ctccgcgatc aacccggcgc gggagtagtc
     1981 gtcgatgaag gcgctgactg tctgtccgtt tccgcccgac cggacgcggc cgtggagagg
     2041 atcgtggtcg atgaacagca tctggcggtc ggggaccccc atggcgcggc agtggatggc
     2101 gtcgtcatcc agccaggaac cggtgaagcc gaggatctcg tagcccggca tggcgtcctg
     2161 gtacgtctgg atggcgacgg cgtcggaggg gctgccgaaa atgggcacca gcactttgcc
     2221 gttcagaatg atcgagttgg tgtaggcggt cccggccggg cagtagacgc ggatgatctc
     2281 atagggccgt ccccaggccg acatctgctg cgagaggtag gcggcgcggg cattcaggag
     2341 ggaatagctg ggatcgctgg gagggacatc cttgacgatg atggtcgccg gatcgaggaa
     2401 tttcgcccag cagtcgatgt ggtggatgcc gcttgactcc acgtaaccca gaacggtgta
     2461 ctggttcccg aggtacgcca gcatgatcga atcgacctgg gccgaggtga ggctcgggtt
     2521 ttcatcgtac acgagccggg tcgacattga cctccccaat ccgtcggaca tgtggttgcc
     2581 gcccgtatgg atcaggtcca tgccgtagac gggaatcccc caggcggagc cgatcgcctg
     2641 cgggatgacg tcgtcgagcg gccgcgggcg gttgtagatg tggtcgacga tgccgattcg
     2701 atcgccggag aatatgaacc aggggccgta gtcgcgcgtc cagatggaat tggtggcggc
     2761 gatgaggaag ttgacgttgg ccatgttgac cccgttcgcc tggtaggcgg cgcgggcctg
     2821 ggcctcgacg gtggtgctgc tgacgatcgt ggtgaccatc agctcctccg acatctcggc
     2881 gacaagcgcg taggagatcc cgaggggcca gcggatgatg acgccgacgg agcgttccca
     2941 ttcggccggg ttgcggagcg aaccggtcgg cgggtaagtc gaccggtggg ccttgccgat
     3001 gtcatcgagg cggccctttt cttcttcggt gagaccgatg gggagcgact catcttcccg
     3061 cccggcggcg gaagcggcga tcccaagcag caggagtgcg gcgagggcaa agagcagtcg
     3121 gcgagacatg cgggtcctcc aatcagcgtt gacgcgcgac ggttgcggcg gacacggtat
     3181 gacaccacca atatacgcgg tccggcgaat tcggcaatca attatagtgc gggagttcga
     3241 tttttgggcg ccgccggaga cgattccgcc cctccggagg ggagccgaac gttgacaaaa
     3301 cgcgggagcg caccgtacct tggacctgtt ccttcccgcc tttgccggac acggctgcgc
     3361 ggcggccggg aggcccgggc ccgcgcggaa gcgtccggcg gcgcaggcgt ccccggcctg
     3421 gcggcggtcc gcggaccgac gaatgacaag cggcagcaag agcgaaactc cgagaggaaa
     3481 atcctccgag acaggaagaa cagccatggg caaccccgac acgtttttga cccgccgacg
     3541 ttttatcgag acctcgctgg gctgcgcggt cacggccggg ctggcgacgc tcgttcccgg
     3601 ccggtctttc ggccaggccg aaccgcccgc gacccaaccg tccactcccg cgcccggatc
     3661 gatcatccat cgcactctcg gccgcaccgg tttcacgttg ccgatagtca acatgggggt
     3721 gatgaacaac aattccccgg aggtggtgcg cgcctcgtac gaactcgggg tccgttactt
     3781 cgacaccgcc gcgcggtacc aaaacggccg gaacgaggag atggtgggcg cggtcatcag
     3841 acaactcggg gtacgggaca aagtcgtcat cagcaccaaa gagatcctgc cgggccgccg
     3901 cgagggagac ggcgccgggg cggtgaagcg caaactggtc gagcagtgcg aagccagcct
     3961 gaaacggctc ggcatggaga cggtcgacat tctctatctc cacggagtgt cgtcgccgga
     4021 ggagatgaaa gaggcggggg cactcgaggc gctggcggag ttgaagaagc aggggaaggt
     4081 gcctgcggtc ggggtctcca cgcaccagaa catggccgcc gtgctcaagg cggcggcgga
     4141 ggggggattc tacgacgtcg tgctcaccgt gatcaatgtc tcgctggcgg acgatgccga
     4201 cctgctcggc gcgatcgcgg cggcagcgcg cgcgggggtc ggggtggtgg cgatgaagac
     4261 gcaggccggg ggggcgcgcc tgcccaatcc ggcgaccctg aaggactatg ccggtgggac
     4321 gattgcgacg gcctcgctca agtgggtgct ccggaacgag cacattgcat cggccattcc
     4381 cggctacgac aatttccagc acatggaaga ggatttttcg gtggcgcgcg ggctcgatta
     4441 tacggcggag gaggcgaagt ttttgtcgga taaccgcctc acgctcggtc tcgctttctg
     4501 ccgccagtgc cggcggtgcg agccgagctg tccgcaccgg gcggacatcc ccgcgctcat
     4561 gcgcacgcac atgtacgcgg cgcagtatgc gaattttgag gaggcgcggg cgacgctcgg
     4621 ggagatcgcg ccggggcgcg gactgcacgc ctgcggggcg tgcgaaaggt gcgcggcgcg
     4681 ctgcgcccac gcggtcgata tcccgggccg catcgagcgc ctgaaaacga tctacgcctg
     4741 aggcgggtcc gggctagccc ccgagcgcgg ccagccaccg ggcgaagaag tcgcggtggc
     4801 gcggggcgta ggcgcgcatt cccgccagcg tgaactgccg caggccggcg gccgccgtgt
     4861 ccgcatacga ccgaatcatc aaggccgcac cgccgtcgaa cggaaggtcg gtctggacct
     4921 tctcggcgcc cgccgcctcc tgccaccgga ggaacttccc gccgctggaa tcggcggcaa
     4981 aaaagaggcg gaccgtgtcg ggtccgagcg cgtacgcctg ctcgtacaca tcggtgagaa
     5041 acgcctgccc gaggtacgag gcggggtaat agcggtcccc gcagggcacg cgggcggaat
     5101 cgggaaagcg cgcgaacgcc gccgggcggt cggtgctccc cggcagcctt cgcatgagcg
     5161 aatccgcggc cacggcgagc atggcgccgg tctgcgaaga ctcgtcgaat gcgatcagcc
     5221 gggcgaggta ccggcccttg acggcggtga cggtcgactc agtgcaaaat ccctgggtcc
     5281 cgatcgccag caggtccggc tcgtccggtc gcaccgcggc gtagaggccg taggcgccgg
     5341 catcgtcggc gaaccggtag acatcgacaa tgaactcaac ctcggggaag cgatagtcgg
     5401 cggtggcgat ctcgacaaag cggtactggc ggtacatctc ggcgtcgccg tcgatgtatt
     5461 cccagagcgt ttcgccggtg aaggtgcgta cctcgccggt cggttccgcc gcccccaggc
     5521 gggtccgaag gaactgcgcc ggtcccccgg ccgtcccggc tgactccttc ccgccgcagc
     5581 cggcgacaga gagggcgagc agggcggcgg ccgccgcggc gcccgcgaac gaatgcttca
     5641 tagcaacctt cctcgatttc gatgccggcg catcttcagc gccggccggt tcagcggctc
     5701 tcccaggaaa acgcgccggg gaattcgtcc ggggcgctct tctccttgcc cgaccaggcg
     5761 tcgatgtcga tccggtagac ggatatttcc tcgatttcct ccggctggat cggccggtag
     5821 tcgacgcccg ggcggaggcg gggaaagtag cggtcgatga gcatctgcat gtgtcgagcg
     5881 gcggcggcgg ggtcgtccac cagcgagatt cgtccgaagg cgatgacgct gcggtattcg
     5941 acggagaagc cgcgggccgt tttggccggc agcagccgcc ccatctcggc gaacgacacg
     6001 cacacgcgcg agccccgctc ggtgttggtg cggaggcggc cggccccggc ggtgtggaaa
     6061 tagagggcct gccggtcggg gtcataggcg aagctcatcg gcgtgatgaa cggctggtca
     6121 cccgcgaccg tggcgatgtg gcagaagggc gcgcgggcga ccaggtcgga aatccaggcc
     6181 tcgtcgcggg cccggtcggc gcggcgcatc cggtggagat cggcgctgtc ggcgcgatgt
     6241 tgatccatgc gatccatcct tgtccgagtg agaggccgcc ggcggaggcg accccgccgc
     6301 aacataggac ggggggggcc gggggtcaag caaaaccgcc ccggccgagg cgcggggcgc
     6361 gcatgtgaag acttgactcc ccgcccgcgc ccgtcctaca ttgcgccatg atcgaaatcg
     6421 caccgtcggt tctcgccgcc gacttcagcc gcctgggcgc ggagatcgcc gacacagccg
     6481 ccgccggggc cgaccgcctg cacctggaca tcatggacgg ccacttcgtg ccgaacatct
     6541 cctacggacc ggatatcgtc cgaaccgtca accgtctgac cggcctgccg ctcgacgtcc
     6601 acctgatgct gaccaatccc gaggccttct ttgagccgtt cgccgccgcg ggcgccgatg
     6661 ccatcacgtt ccacatcgag gtccaccccg acccggtcgc ccatctgaaa cgcctcgagg
     6721 aaatgaaggt ggcgcgcggg ttgtcgctca atcccgacag gacggccgag tccgtgctgc
     6781 cgtttctgga actgtgcgac caacttctgg tgatgtcggt gtttccggga ttcggcggcc
     6841 agcggttcat cgaggagacc ctgcggacag tcgaggccgc ccgacggtat atcgatcgcc
     6901 accgcctgcg cacgcagatt gcgattgacg gggggatcga cggcaacaac gcgcagcggg
     6961 cggtagacgc cggcgccgac ctgctgatca tgggatcggc cttttaccgg agcaccgacc
     7021 gggcggggtt ggtgcgaaag gtccatgggc tgaaacgggc aggatgaagc ggggggcgta
     7081 cttgccgccg gtattcctga ggacttgtag cacattgtgc aacagcagca tggctcgctt
     7141 gcggcggggt ggcggcggcc ggatagctta ggggagccac gaaaagggcg gagttccggt
     7201 tctgattcgt gcgggaggag cggccggtcg gcggggaggc cggttcccgg tctctattgc
     7261 ggcgccggtc cgcccgcggt ggggggggga gggcggcgga cccttgtgcg cgcggccggg
     7321 tcgagcgtat gttaataatg aatcggaggg ttcctgtcga tgtagtagtg agtgcgggcc
     7381 aaggtcgacc cgccgactga acacggaggt acacaggtgg caaacgtgaa gaccaagaag
     7441 ggcgtgatcg gcatactgac cggcggcggc gacgtccctg gtctgaatcc cgccattcgc
     7501 gccgtcacca tccgcgccat ccgcgaaggt taccgggtga tcggcctccg ccacgggtgg
     7561 gccggaacga tcgacatcgt gcgcgacaag aagcacgaca acagcggggc ctttgtagag
     7621 ctgacggaag agatcgtgaa caaggcgggc cgcaccggag gaacattcct ccacagttcg
     7681 cgcacgcatc cggggcacgt cacccaggag aacatcccgc cccacctccg cgacaagtac
     7741 aaaggggcca agagcgatct gacgcccgag gtgatcaaga acctcgactg gctcggcatc
     7801 gactacctga ttccgatcgg gggcgacgac accctgtcct acgccgtccg cctctacaaa
     7861 gagggcgtca agatcgtggc gatcccgaag acgatggaca acgacgttcc ggggaccgac
     7921 tactgcatcg gcttctccac ctgcgtgacg cgcacgatca tgatgaccaa tctcctgcgc
     7981 acttcggccg ggtcgcacga gcggttcctc gtgctcgagg tgttcggccg ctacgccgga
     8041 ttcaccgcca tgcttccgac aatggccggg gcggccaacc ggtgcgtgat tcccgaacat
     8101 cagttcaaca tcgacctgct cgccgaattg ctcacctatg accgcaacca caaccccagc
     8161 cgctacgcgg ttgtgctggt ctccgaggga gcgatgttcg aaggcgggga gatggtgttc
     8221 caggattcgg ccgccgacgc ctatgggcac aagaaactgg gcggcattgg cgacctggtg
     8281 tcggcggagc tgctcgtccg gtcccccaag tacaacaacg gcaagcaaat ccggtgcatc
     8341 acccagaaac tcgggtacct cgtgcgggga ggggatcccg acgctgtcga ttcgatcgtg
     8401 ccgatggcct atgggaacct ggcgctggat ctgatcctga agaacattca cggacggctg
     8461 gtcgtgctga agaacggccg ctatgacgat gttccggtgg atgttgtgtc gagcactaag
     8521 aagctggtcg acgtcgagcg gtactacaac cgggagcggc tgcggccgtt ctatcactcg
     8581 ttcgagatgc agccgctgtt tatcatgacc agcgagtaga gcgatcgcgg cgagtcaata
     8641 cattcagacg gccggacaat cgtccggccg tttctgtggg tggccgagca caggtagggg
     8701 ccgaccgggc gaaaggtgta agattctgta atacaacaag ttgtgctggt attagtgagg
     8761 ccggcggaga ccggtcgccg gatgaagcgg agacagcggg aacgcggggt gggccgataa
     8821 ttgtcatttc ttcgcggttt gccttgacag cgggcgtggc cccgctatat tcggacttgt
     8881 gaaaaaagta acatatcgcc ggtgtctgtc cggcggccgg cgctggttcc aaggggcaaa
     8941 caagtacttt tagaaacacg gcggttgtcg gccgggccgg gtcaatcgcc acagcatgag
     9001 tgatgagagg tcgtggtcat gagtaaaggg tcattcaatc catttttgat ggcgcaaacg
     9061 cagttcgacg gcgttgccga catcctggaa ctggacaagg cgacccgtga acttctccgc
     9121 aacccgctgc gggaatacca gttcagcatc ccggtccgga tggacgacgg ggcggtccag
     9181 gttttccggg ggttccgcgt ccagcacaac gactcccgcg gtccgtgcaa gggcgggatc
     9241 cggttccatc cgatggagac gatcgacacg gtgcgcgccc tggcgatgtg gatgacctgg
     9301 aagtgcgcgg tggtcgacat tccgctcggc ggcggcaagg gaggcgtggt gtgcgacccg
     9361 cacaacctga gccagcgcga gcaggaagcc atctgccgcg gctgggtccg ccagttggcg
     9421 aaaaacgtgg ggccgatcgc cgatgtcccg gcgcccgacg tcatgaccag cccgcagcat
     9481 atgctctgga tgctcgatga attcgagcac atccacggcg gcaagtatcc ggggttcatc
     9541 accggcaagc cggtggggat gggcggctcg ctcggccgca ccgaggccac cggcttcggc
     9601 gtcgtgttca cgctgcgcga ggcgctcaag cagctccaca ttcagccgga caagacggcg
     9661 gccagcgtgc agggattcgg caacgtggcg caatacgcca tcaagctcta ccagcagctc
     9721 ggcgggacgg tgattgcggt ttccagctgg gaccaggcgg accagacctc gtacacgttc
     9781 cggcgcacga gcggcatcga tctccagcag ctcctgggca tcaccgaccg cttcggcggc
     9841 atcgacaagg gtaaagccgc cgggctcggc tacgaggtgc tgccgggcga ggcctggctg
     9901 gagcaggagg tcgacgtgct catcccggcc gccctggaaa accagatctc gggcgagaac
     9961 gtggagaaga tctcgcggcg ggtgaaggtg attgcggaag gggcgaacgg tccgacgaca
    10021 ccggaggccg acaaggtcat ctcggcccgc aacatcttcg tcatccccga cttcctggcg
    10081 aacgcgggcg gcgtgacctg cagctacttc gagcaggtgc agtcgaacat gaactactac
    10141 tggccgaagg aggaagtgct gcagaagctc gaccagaaga tgacggcggc cttcctggcg
    10201 gtcagcgagc tggcgagccg caagaagctg tacatgcgcg acgcggccta cgtgatcgcc
    10261 gtgggccggg tggcgcaggc ctgccgggat cgcggctggg tctgagattt agtgcggtga
    10321 acgcaagtca acggccgtgc gcagcccgcg cgcggccgtt tcctgttgca ttgggcgcgt
    10381 tcccgcgcga tattgtcgcg aggggcaggc cgatgcgggg cgcaggccgg cgccgccgcg
    10441 ccgcaggccg ccccgccgac gcgaaggaat cacagctcta tgaccgatga acagaaaccg
    10501 ataggagact actgggtgtt ggatcgcctg atcggctctc tccaggagcg ggccaaggag
    10561 ctcaactgca tctaccgggt gggcgcgatt ctcaccaatc cggacgagcc gatcgggcgc
    10621 gtctgcccgc tcctcatcga agccattccg accggctggc agtaccccaa gctgtgccag
    10681 gtcaagatcg tcattgagga caacatcttc gccaccccga acttcatcga gacgccctgg
    10741 ctgcagtcgg ccgatatcgc cgttcaggat atcgtcatcg ggtcgatcac ggtatcctac
    10801 cgcgaggaga cgccgagcgc ggccgagaag ctgtttctcg ccgatgagtc gcggctgctc
    10861 aagacgatcg ccgagcgtct cggatatttc atctgccacc agaagatgcg gcagatcacg
    10921 gaggagtggc agacagtccg ccccgaggtc tcggtcgggc gccccgagtg gcaggtggtg
    10981 ctgaaactcc tgcgggacac ggatcgggag ctctaccaga cgatcgcgcg gaagatgctc
    11041 aaccacctgt gctggagcgg catcgccgag gccgagaaac tcctccgctc gttcacctcg
    11101 aacgagtcgc tggaggacga ggaaaacccg gagcactgga accgaccgca ccagcgcaaa
    11161 tcgctggtga tgtcggcggc tttcagttcg gcggctttca aaatcgcggc cgaccacctc
    11221 gacgccggcg agatcctcaa cctcatccag aagtggatcc aggaggacaa gctcggcttc
    11281 ctcgtccagg cggtcaaccg gaacctgacg ctgtcggagg tggccgacgc cattcggcgc
    11341 taccagcacc tcacccagga cgagccggtg acgctgtcgt cgtcgatgcg ggggatccag
    11401 gtgtcgctga tccggcggtt cctgtctgac cagcagcagt atatcaacgt ggcgaagaac
    11461 tatgtcgatg tcgccgactt ctgcaagctc atccagaacc tgatctacac atccgagagt
    11521 tacgggcggc tggggggcaa gagcgccggc tttttcctgg ccaagcagat tttgctcaag
    11581 aaagccgcgg acaacgagct gctggccaac gtgcagatcc ccaagacctg gtacatcacc
    11641 tccgacgtca tcctctacta catgcactac aacaacatgg acgacgtggt ggagcagaag
    11701 tacaaggaca tcgggcagat ccgccgcgag tacccgcacg tcgtccagtc gttcaaaaac
    11761 gcccgcttcc ccgccgacat catcaaggga ctctcgatgg cgctcgacga cctgggggac
    11821 cgcccgctca tcgtgcgcag ctcgagcctt ctggaagacc gggtcggggc ggcgttctcc
    11881 ggcaagtaca agagcctctt tctggccaat cgcggctcca agcagcagcg cctggacgcg
    11941 ctgacggatg cgatcgccga ggtctatgcg agcacgttcg gcccggaccc catagaatac
    12001 cgcgccgagc gcgggctgat tgatttcgcc gaggagatgg ggatcattat ccaggaggtg
    12061 gtggggcggc ggatcgggaa gtatttcctt ccctcgtttg cgggggtggc tttcagcaac
    12121 aacgaattcc gctggtcgcc gcggattcgg cggacggacg gtctcatccg gctggttccc
    12181 ggcctgggca cccgcgccgt cgaccggctg agcaacgact acccgatcct gatcgcgccg
    12241 gggcagccga cgctgcggac gaacgtgacg ccggacgaga tcgcgcggta ctcgccgaag
    12301 tatatcgacg tgatcaatct ggagacggag gattttgaga cgattccgct gctggacttt
    12361 ctccgggagg tgaatttcga agttccgggg atccagcaca tgctgtcgct cagcatggat
    12421 gggcggctgg tggcgccggg cctgacggcc gatatttcca gcggcgcgcc ggtggtgacg
    12481 ttcgacggcc tggtgtcgcg gacgcgcttc ctcaagcgtc tgcgggcgat catgcagacg
    12541 ctcgaggtca ccctgggaga gccggtggat atcgagttcg cgtgcgacgg ggatgatttc
    12601 tacctgctcc agtgccgggc ccagagctac tcagcggaaa gcgccccggc ccccattccc
    12661 aaggatatcc cgatgcagca gattgtcttc tcggccaacc gctacgtctc caacggccgg
    12721 gttccggatg tgacgcacgt cgtctacgtg gacccgcgga agtacgccga gatcgaggac
    12781 cgggccacgc tggtggcggt ggggcgggcg gtcagccgcc tgaacaagct gctgcccaag
    12841 cgcaaattca tcctcatcgg accgggccga tgggggagcc gcggcgacat caaactcggc
    12901 gtcaacgtca cctactccga catcaacaac acctccatgc tcatcgaggt ggcgatccgc
    12961 cacggcaact acgtgcccga cctgtcgttc ggcacgcact tcttccagga cctggtcgag
    13021 gccaacatcc ggtacctgcc gcttttcccg ggggacgatc acgtggtgtt cagcgagcgg
    13081 tttctcaccg aggcgccgag cgtgctgccg gagctccttc cggagtgcga cgagctggcg
    13141 gaggtgatcc gcgtcatcga cattccccag gcgacggggg ggaaggttct ctccgtgctc
    13201 atgaacgccg acctggacga ggcggtgggg atcctgacgc cgccgggggg cgggctggag
    13261 atcgcggcct cgccggcgga atcccccgcc caggtgcgcg gggatttctg gcggtggcgc
    13321 ctgcagatgg ccgagcagat cgccgcccgg atcgacccgg tccggttcgg cgtggccgcg
    13381 ctgtacctga tcgggtcggc caagaacgcg aacgcgggtc ccgacagcga tatcgacctt
    13441 ctcgtgcatt tccgcggctc ccccgagcag cggcaggacc tggtgtcgtg gctggaaggg
    13501 tggagcctca gtctcgacga ggtgaatttc atccgcaccg gttatcggac gggcggcctc
    13561 gtcgatgtac acatggtgac cgacgacgag gtgagcgcca agtcgggtta cgcagcccgt
    13621 atcgacgccg tgaccgatcc ggcccgtccg ctggcgatgg cgcagcggca gccggcgggc
    13681 aaaggaactg ccgggcacta ggcggcgcgg ggagcgcccc ggcgccgccc cgggccgccg
    13741 gccgggttct cactcttcgc cgaacacgag cgcctgaaac cgctccactt tcgcctgcgg
    13801 cgacttgtag gcatcgggcg acaagcccgc tttgcggcag gtctggcgca gaaactcctc
    13861 ccgtgtccac ccgtactctg tcgccacctg cggcagcaga agcccgcggt tcgggccgag
    13921 tgaaatgatc aggccgtccc gcccgacctc gatttcctcg aatattgtca ccgcctgcag
    13981 cggggtgagg accgagatct caatcgtgag ttgatcgatt tcggaccggg tgacgggttc
    14041 gaaccgcggg tccccgacgg ccgcctgcac ggcgcaggcc gccaccgtcc ggaagagggg
    14101 atcggcggcg acgacatgcc cgatgcatcc gcgcagccgg ccctgtttct cgagcgtcac
    14161 gaaggcggcg ccgggttcgg ccagcttgcc ggccgcctcg aaatccggtg ccgcgccgtc
    14221 ctcgagatac ccgatgatag agcggcgcgc gatcgtcagg agcgtctgcc gctcctcctg
    14281 ggtcagatca tatggttcca tacctttttc cggggcagcg gcttcacccg gcgcggccga
    14341 gttctcgccg tcggcgctgc aatagaaggc gacggccgcg tatcccacga ccgagctctt
    14401 gtcgccggtg agatcgccgg agtcgccgta gccgaggatt ttcactttat cggcgccgag
    14461 ttttcgggcg gcgcgcatta cggccacgac ccccgctccg ccgcaggcct cgaccttctt
    14521 cgcctcgagg ttgcgcagca gcgcgtcggc atcgaaagct gcgaggcagg ccaggccgag
    14581 cgaatccatc gggtacccgc cggcggccgg gcggtagtgc tgccagtcgg ttgaggcgat
    14641 gagcacggcc ccggggggcc gggcggcggc aacgagcgcc gcggtcagcc catcgaccgt
    14701 gtggccgtcg gggtagccca tagcgatcgg gactatgtgc gcgccgggga ggacggtttg
    14761 gaggaagggg agctgcacct cgagcccatg ctccttgagg tgggcctcgg ggacgacttt
    14821 gagccaggag gaggcgccga ggaggcgttc gatgcgggcg tcatcgcacg gcaccgggcc
    14881 cagcggcgtc tgccaggtca ctcccggccc gtaaaccgag gcgccgcgga acccgtagcg
    14941 gtgggcgggg ccgcagagga tggccgtttc gatgggccgt cccgcgagcg ctttgtaggc
    15001 gtgcgcggcg atcggccccg aatacacgag ccccgcatgg gggacgacga tggccacgat
    15061 gcggccgtcg atggcggccg cgggggcggc ggccaactgg ccggcgatca tggccgccag
    15121 ctccgcgctg tcgccggggt agaaggaacc ggcgaccgcg ggcgggcggg tgacgggcgg
    15181 cgcggatgcg gccagagcag ccgccgccgg cagcacgagc aggaaacaaa cggtgaagcg
    15241 tgtcatgagc actccccgat ctccggcggt gcgggcccgc cgggcggaaa gaatttgtcg
    15301 ttctccggcg ggccgtatat tattaagata tgcagcgcgt cgttgtggtc aagtcagtat
    15361 ctttttccgg cggacggcgg ccgggtccgg agacctaccg gcgcctgctg gaggtcggcc
    15421 tggcgcacct ggcgggcctt cccccggcgg cggcgctgcg cagcctgctt ccggggggca
    15481 caatcgggat gaaggtgaac gcgctggtcg ggccgcgcaa ttcgacgccg cccgcgctca
    15541 ccgccgccgc cggggacctt ctcgaagcgg ccggctgggc cgccggcgac ctgatcgtgt
    15601 gggaccgctc caaccgcgag cttgaccggg cgggtttcac tctcaacgtg gggggaaatg
    15661 ggcggcgctg tttcgggacc gacaccgccg gcttcggcta caccgccgag atccacacgt
    15721 acggggaggt cggctcgcgg gtgagccgcg tgctcaccga ggccgtcgac tcgaacatca
    15781 acctgccggt gctcaaggac cactccattg tcggtctctc gggcgggctg aagaacatgt
    15841 acggcgcgat tcacaatccc aacaagtatc atgaccactg cggggatcca tactgcgcgc
    15901 atgtctcgct gctggagccg gtgcggcgga agaaccggct gacgatcgcc gatgcggtgc
    15961 tcgtgcagta ccacggcggc ccgggtttcc gcgaggagca cgtggcgccg tacggggcgg
    16021 tcctgctgtc ggatgatccg gtggcggtcg accgggtcgg cctggagatc atcgaatacc
    16081 tgcgcgcgcg ggcggggctg gggacgctgg cggaggacaa gcgggcggtg acctatctcg
    16141 ataccgccgc ctgcgggctc ggcgccgcgg acctcgcccg gatcgacgtg atcgtgcgcg
    16201 ccgtcgatgg ggccggtcgg gagaccagcg gggagttgtt ctcatgaacc ggcggagctt
    16261 tctgcgctgc ctcggctgcg gggcggcggc gacggcgctc ccgcacgccg acttcctccc
    16321 cgacctcctc ggggggatcg agaatgcctg cgccatcgac ctgagcgacg cgctgtcgtc
    16381 ggaggaggcg cgccactata cgaagctggc gggcgggggc attgagtgca ggctctgccc
    16441 gcgccgctgc cgcgtcaccg acctcgagcg cggctactgc ggggtgcggg agaaccgggg
    16501 ggacacctat cacacgctcg tgtacggcaa gccctgcgcc gtgaatgtcg acccgatcga
    16561 gaagaaaccg ctcaatcatt tctacccggg cacggcggcg ttttctctgg ccaccgccgg
    16621 gtgcaacgtc aactgcaagt tctgccagaa ctgggagatt tcgcagtccc gcccggagca
    16681 aaccaagaat ttcgatctgc cgccggaggc ggcggtggcg ctctgccggc agcagggcgc
    16741 gccgacgatc gctttcacct acagcgagcc gacggtgttc tacgagtaca tgtacgacat
    16801 cgcgcggctc ggccgggcgc aggggatccg gtcggtgatg atcacgggcg ggtacatcga
    16861 agaggctccg ctggccgagc tgatgccgca tctcgacgcg atcaaagttg acctcaaggc
    16921 gatccgcgcg tcctactacc gcgacgtggt gcgcggggag ctcacgccgg tgctcgagcg
    16981 tctgcagcag attcgcaagg ccgggctgtg gctggaactc gtctacctgg tggtgccgac
    17041 gctcaacgac agcgacgccg agttccggga actcggccag tgggttttcc ggtatctggg
    17101 cgcggatgtt ccccttcact tttcgcgttt cacgccgcag tacctcctgg agcaccttcc
    17161 gccgacaccg gtggcgacgc tcgagcgggc gcacgcgatc gccggcgccg agggattgcg
    17221 cttcgtatat gtgggcaacg ttcccggcca ccgggcggag tcgacctggt gccccggttg
    17281 cggcgccttg gtgatcgagc gccgcgggta ccgcacctcg gccgcgggcc tggccggcga
    17341 ccggtgcgtg gcctgcggga gggaaatacc cggacggttc aaatgaaccg ttcggcgttt
    17401 ctgttgggtt tcgtagccac cggcggacaa accctcctgc tgcgggaact ggtgtcctcg
    17461 ctcgggggga gcgagctgtt catcgggagc gcgctcttcg ggtggctggc ggcggtcgcg
    17521 gcgggagcct gggcgggggg tcgatggccc ggccgcctgc gggctgcgct cctgctcgtc
    17581 ctcgcctcgc ttgtgctccc gctctgcgtg gccgctgccc gcctcgcgcc gctcgcttac
    17641 ggcgtggcgg tcggggaggc aatcccgctc gtgccggccc tgaccatctc cttcctcgtt
    17701 atggctcccc ttggggtgct ctcgggctgg ctctttccgg ccgtggccgg ggagcacggg
    17761 gcgccggccg atggaatcgt ccgggtgtac ctgttcgagg ggataggcgc ctttgcggcg
    17821 gggctcgcgg tcgtgctcgc ggccgggacg gtgctctcga cgccggcggt cgcgctggcg
    17881 ctggtggtga tcgtcatggc cgccgcgctc ggcggcggcg cgtcgatgcg gacggtccgg
    17941 ggggcggggg cggtcctggc ggcggttagt ttgctggcgg cggtggcggc gctcgaccat
    18001 ccgctcaacc gcgcgctcga ggccgccaag tatgccggct acgcggtcgc ggcgtcgttc
    18061 gacactccct acagccacca ggcgctcctc ctgcgggatg gcgcctgggt tttgctcacc
    18121 gacaatgcgg tggaggcaac ccgaccggac gcggccgcgg ccgagagtca cctgctgccg
    18181 gtcctggcct accggcccgg ggcgcgcgag gtgcttttca tcggacggac cgaattcgga
    18241 gtgagcgcgc tggcccgcca acttcccgat ctgacggtga cttcggtgga cccgcgcgcg
    18301 cggctcactg cggcgctggc cgagcacgga ctcgccggag aaggtgatcc cccggtgcac
    18361 gagctgccgc ggcgatttat cgcggggggg accgggcggg gggagttcga tgcgatcgtg
    18421 ctcgaccccg gcgacccatc gacctatcgc gcggccggcc tgctgacccc gcagttcctc
    18481 ggtcaacttc ggcgcgtgct gaacgactcc ggggtcgtgc ttctggcgac ggcctacgat
    18541 accgaccggt ttgtcgggcg gcgcgaggcg gaattgctgg cgaccctccg ggcatcgttc
    18601 gcgcaggtgt tcgcccacgt catcctgtgg ccgggggagc agacgctgct tctggcatcc
    18661 gatgcggcgg tattcgatgt cccgctggat acggtttttg cccgcctcga cagtctgccc
    18721 tgccgaccct tgtgggtgaa ctcgtcgtac ctgtttgacc ggctcagccc gctgcggacg
    18781 gagcggctgg aggcggcgct cgcggggcag ggagcgccga ccacgatcga tcgccccgcg
    18841 ctgacactgg agcatatcgg tttccgggcc gagcggcacc cggtcgatgc cgcgatcgtc
    18901 gggctgaccg gctcgccggc gtggtgggcg gtcggcgtgc ccgcggcgct ggtcggcctg
    18961 atcgtctggg gcgccgccgg ccgacgggcc ggccggcgct acggcctggt cctctatttc
    19021 gtcgccggtg ccgtctcgct cgcggccgag cagctggcgt tctacgtgta ccagtcgcgg
    19081 gcggggtctc tgtactccga aatcgccctg ctgatcggcg ccttcatgct cgggctggcg
    19141 gtcgggacgt ggtatgcggc gcgccagccg cagcagcgcg gtctgatctg gccctcgctg
    19201 tcgactctgg ccctggcgct ggcgatctac ctgactctga ccggccggat agatccctcg
    19261 gcctttctgc tctaccacat gctgttcctc tttgtggcgg cggccgccac ggggagtctg
    19321 ttcgtggcgg cgacgcggcg ctgctatccg agcgcggccg aggccaaccg ggggctcggg
    19381 tatgcgtgcg agctggccgg ctcatcgctg ggcgccctgc tggtcccgac cgtgctgctc
    19441 ccgctcttgg gtgtcccggc gatactgatc ggtctgatcg ggttggtcgg gctggccggg
    19501 gtcggggcgg cgattgtcga tcggcgcacg taggcgcgcg cggttgtgcg gcggccggct
    19561 cgcccctata ttgcttcaac gacaccacct cttgcggaaa ggacgcgccc catgcgggaa
    19621 gaattgcgca ccggttgtat ggatatccag cgcggccggg ggcgggatga tgccgcccgg
    19681 ttgcggcgtc tgctcgaggc ggcctgggat tgggcgatgg ccgatcgccc cgagtgggcg
    19741 acgagcacgg gttatccgaa gtaccatgac cgttggacag actgctccga gggcgctttc
    19801 gcccggcgac gcgaggcgac ggcgctcctg atcgagacgc tcgccgccat cgaccgggag
    19861 aagctctccg cgcgcgaccg gctgagctac gatctctttc tctggcggca ggagcaggac
    19921 cgggcgcgcc agcgcttccc cgaggagctg ctcgggctca accagatgga cggagtgcag
    19981 caggacgcgg cgcgcatagt cgggctgatg ccggtggaaa ccgcggccca gcgcgacgcc
    20041 gtgctcgccc ggctggaagg gctgccgctg ctggtcgagc agacgatagg gctgctcgcg
    20101 caggggctgg cgcgggggat cacgccgccg cgagaaccgc tccgggaggt tccccgccag
    20161 gtgctcaacc agatccccgc cgcggcggcc gaggcgccgc tgttgtcggt gctgcgccgg
    20221 ctgccgacgg ctctgtcggg ggccgcccgc gagcggtttt gcgcccaggc gacagcgctg
    20281 tttgcggagc gggtggcgcc ggcctttcgc ggcctgcacc gtttcctcga ggggtcctac
    20341 ctgccgggct gccgggaaac ggtctcctgg cgcgatctgc ccgggggcga cgactggtac
    20401 cgcacgctca tccggtacta cacgaccagc gagctgacgc ccgacgcggt attcgaaacg
    20461 gggatggcgg aggtcgggcg catccggggc gagatggagc gcatcatagc ggagaccggt
    20521 ttcgcgggcg acttcgccgc gttctgcgag tttctgcgca ccgatccgcg tttcttctgc
    20581 acgagcgcag agcaactgct gagcacgtac cgcgacatcg ccaagcgcat cgaccccgag
    20641 ctggcccgcc ttttcggacg gctcccgcaa ctgccctacg gagtcattcc gatcccgccg
    20701 tacgcggaga aatcgcagac cacggcgtac taccagccgg gatcgctgcg ggcgggccgg
    20761 gccgggtact tctacgccaa cacctataac ctggcctcgc ggccgacctg ggagatggag
    20821 gctctcacgc tgcacgaggc ggtgcccggc caccacctgc acattgcgct catgcaggaa
    20881 atggaggatc tgccggagtt ccgcaggtat tcgtggctgg aggcatacgg cgaaggctgg
    20941 gcgctctacg ccgagagcct cggcgaggag atgggattct accgcgaccc ctatgcggaa
    21001 ttcggccggc tgacctacga gatgtggcgg gcgatccgcc tggtggtcga tccgggcctc
    21061 cacgccaggg gctggacccg gcagcaggcg atcaagttct tccgggcgaa ttccagcaag
    21121 gccgaccacg atatcgcggt cgagatcgac cgctacatcg tctggcccgg ccaggcagtg
    21181 tcgtacaaga cgggcgagat gaaaatcaag gccctccggc gccgggccga agagcgcctg
    21241 ggggagcgtt tcgacatccg cgccttccac gatgaactgc tcgcccacgg ctgccttccc
    21301 ctgaccgtcc tggaggagcg gatggcgggg tggctggccg ggcaggaggc gccggcggcc
    21361 cggtgagacc ggtcactggc ggaggcggtc gatatcttcc tctttgccga ggatgatgag
    21421 caggtcgtga tcgcggatga cgtagtcgcc gccgggggcg aagatgtagt cgccggtgcg
    21481 ggcgtgtttg gtggcgatga cctggatgtt gaagcgggcg cggagctgga tgtccctgag
    21541 cgacttgccc acgaaactgt ccggggggac gagttcggcg acgaagtgct cggccgagag
    21601 gggcaggtag tccagcaggt tggactgggc cagggagtgg gacaggcgcg aggccatctg
    21661 ctcctcgggg atgatggcgt cgtcggcgcc gaccttgatc agaatcttgg cgtggtcggc
    21721 ggagttggcc ttgacgatca cctggcgggc gcgcagctct ttgaggtgga gggtgatgag
    21781 gatggaggcg tgggaatcga cgccggtcga gacgatgaag gcgtcgaagt tctcgacctc
    21841 gaggtgttcc aggaagcggc gttcggcggc gtcggccaca atggcgacgt ggacgatgtc
    21901 ctgcagatcg cgcacccggg cctcgttggc atcgaccgcg gtcacgcggc agtggaggcg
    21961 ggtgagctcg cgggcgacgg tggcgccgaa attgcccagg ccgatgacga cgaattcttt
    22021 cataagccca accagtttaa actgttgttc cctctgcggg acggcggccg cgctcagccg
    22081 atggcgatcg gttcctcgac ataaacgatc tccccggtcg cggtcggccg cgccaggccg
    22141 aaggccagcg tcagcagtcc gacgcggccg atgaacatgg tcaggatgat aacgattttt
    22201 cccggcatat tcaattcggg agtgatgccg agggagaggc cgaccgtgcc gaaggcggag
    22261 acgacctcga agaggttttc ggccagccag ccgtggacgg agccggcgcg cagggggggc
    22321 cggtcccaga ggacggcgag cagggcgagc atggcggcga cgacgaacac cgcgaggatg
    22381 aacacgccga ctgcgcggat gatggactcc tcgccgatgc ggcggtggaa gatcgacgga
    22441 gtgcggcgtc cccggaagcg gtcccaggcg gtggtgacaa tgacggtaat ggtggtggtc
    22501 ttgatgccgc cggcggtcga gcccgggcag gcgccgatga acatgagcac catggagacc
    22561 agcacggcaa cctcggtgag ccgggcctgg gagacggagt tgaagccggc ggtgcggcag
    22621 acgaccgctt ggaagaaggc gttgacggct ttgccggccg ggctgaaacc cgccagcgtg
    22681 ttgccgtact cggcaaagaa gaccgccagc gtgccgccga cgagcaggac cgcggtcgcg
    22741 ctcaggcaga tgcgcgtgtg cagcgagagg cgacgggact ggccggaggc gcggtggagg
    22801 agctcggtaa tgacggcgaa cccgagcccg ccgaggataa tcagggccgc cacggtcagc
    22861 aggatggccg gccggtcggc gcaggcctca aggttggcgg agaaagtcga gaacccggcg
    22921 ttgcagaagg cgctgaccga gtgaaagacg gcgtgccagg cggcctgccc gagcggcagc
    22981 gacccgctga agaggaggaa gaggaccagg gcgccgatca gttcgatggt gagggtgacg
    23041 ccgatgacgg cctggaggag cgacttggtg cgcatgaagg tcccggcggc gagcgacccg
    23101 gccacgccga gccggtcgcg gaaagcgacc tgtgccccgg cggcggcgat caggagagtc
    23161 gcgaacgtca tgataccgag cccgccgagc tgaatcagaa cgaggattac gacctggccg
    23221 aagggggaga acgccgtgcc ggtgtcgacc acggtgaggc cggtaacgca cacggcggag
    23281 gtggcagtga agagggcgtc gatgaccccg accggccggg cgccgtgact gagcggcagg
    23341 ctcagcacga acgcgccgag caaaatggcg gccaaaaagt agaggattgc tttccggccg
    23401 ggatcagcgc cgaggaactc catcgctggg ggccaatatg gccgcccgcc gacggacctg
    23461 tcaaggtctg aatggcgcac taagatttcg ctcgaccttt gcccgccggc ctgtatcttc
    23521 tgaggtaatg ctgaacaagg tgcgacaggc gattcgggac ggccatctcc tggcgcccgg
    23581 cgacgcggtc ctggtcgggc tttcgggcgg accggattcg gtggcgctgc tgcacgccct
    23641 gtgggcgctc cggaaggagc tcggcctggc cctgaccgcc gtgtacatca atcacgggat
    23701 ccgaccggcg gctgcgaaaa gggaggagcg cttctgcgcc gccctgtgcc ggcagctcgc
    23761 ggtggaactg acgatcgtgc gcgaggatat cccgatgctg gccggacggg agcggaaggg
    23821 tctggaggag acggcccggg actatcggta ccggatctat gagcggctcg cggacgagcg
    23881 gggctgccgg cgcatcgcac tcggccacca ggcgaatgat caggcggaga cggttctgtt
    23941 ccgcgtcctg cgcgggacgg ggcggcgggg gctggcgggg atcccgcgga aacgcgggcg
    24001 ggtgatccgg ccgcttctcg atgtgtggcg ggatgagatc ctgggctacc tgaaggagca
    24061 tcgcctgact ttctgccgcg acgagagcaa ccgggacctc cggttccggc gcaacttttt
    24121 gcggcacaag gtcatgcctc ttgtccggaa gagcttcaac ccgaaggtag agcgggccct
    24181 tgcggatctg gccgatacgc tggcggcgga ggaagagtac ctcgagggag tgctggccgg
    24241 ggtgcagaag aaggttctca cagagacgcc ggggggcaag tttgagcttg atttgcggcg
    24301 gtttgatggt tatgataaat ggatccgtcg ccggctgtta aggtattgtc tttcaaaaat
    24361 ttcaagcgac ggaacgagcc cggacaaaga ggtggtggaa cgcctcgacc gggctgccct
    24421 tgccgggcgg gcgatgctgt cgctccccgg cggcgcgcgc gccgagcgca tcggcgcagt
    24481 cctggtgctg ggccgtcgcg tcggcgcctt tcgcgccgaa ttgactccgg gcgtaccggc
    24541 gaagctggag cggccgcggc tgaatatcac ggcccgcatc cggcgcggcg ggtcgtcggc
    24601 cattcgaccg gtgcgccgag ggcggaaggt ctatctggac tgggaaaagg ttgtgccgcc
    24661 tttggaggtg cgctcgattc ggccggggga tcggtttcgg ccgctgggtc tgggcggccg
    24721 caagaaagtc ggggacttcc tgaccgatcg gaaagttccc cggctgcggc gggacgagat
    24781 cccggtggtg gccgaccgcc acggcattgt ctggctcgtg ggttgggaaa ttgcagagcg
    24841 ggtaaagata gatagtgcaa cgaggaaggt gctgacaatt gagtgcagcg agagattacc
    24901 aggccaagcc gaaaccgttt gagttgctcc tggcacagga tcggatcgcc cgacgcatca
    24961 acgagctggg tcaggcgatc acccgcgatt acgccgggcg cacccccgtg tttctcggcg
    25021 tgctcaaagg gtgcgtggtg ttcatggccg atctcatgcg ggccgtcaaa ctcccgctgg
    25081 aggtggagtt catttcggcc gaatcttacc atgacgggac cgcgcagcag gaattgaagc
    25141 tggaccactg gttcacgcgc tcgctgcgcg gccgggacgt gctgattgtc gagggcgtcg
    25201 tcgactccgg ccggacggtg gaggcgatca gccgggaact ggcggccgtg gaaccggcgt
    25261 cggtcgaggt tgttaccctc attgacaagc cggccaacca ccggcgcaag ctggtgatca
    25321 agtatgtcgg gttcgcgctg ggcaacgagt tcgtcatcgg attcggcctg gacaacaccc
    25381 agaggtaccg caacctcccc ttcatcggcc ggatgatcga ggagtgagcg gtcgggggag
    25441 acacgtgaat tcccgggcgc ctgccgggta taagagtcag ttgcggctaa cgaagaaaac
    25501 acagcggagc ataggtgtct agtcataagc caaagggttc cccgccccca ccgggttcgg
    25561 ggagagagaa ccggccggac ggatccaagc agccgcccgg actgcggggg gcgggccgat
    25621 ccctcatgtt ctggatcgcg ctggccctgg ccgtcgtcgt catctactcg tacatgaact
    25681 ccttctcggg tgaccaggcc gaggtcacct actcggagtt catggccgag gtcgagagcg
    25741 gcaacgtcgc cgaggtcacg ttcaaggatc gcgaggtcca gggcagtctg cgcgagccga
    25801 aagcgttcga atcgatccag cgggggcaga cggtgacgaa attcaagacc cggattccct
    25861 tcccggacta caactacggg ctggtggacc gtctccagaa gcagaacgtc aagattatcg
    25921 cgaaaacgga gagcccgctt ttgggctacc tcctggcggc ggctccctgg ctcctcatcg
    25981 tgctcgtctg gctgttcttc atccgccaga tgcagggggc gtccggtccc cgggggctgt
    26041 tctcgttcgg caagagcaag gccaagctgc tgaccgacga gcggccgaaa gtgacgttca
    26101 aggacgtggc cggggtggtc gaggcgaaag aggaactgag cgagattatc gattttttga
    26161 gggagccggg aaaattccag aggctgggcg gcaagatccc caagggggcg ctcctgctcg
    26221 ggccgcccgg cacggggaag acgctcctgg cgcgggcgat cgccggcgag gcgggcgtcc
    26281 cgttcttcag tatgtccggg tccgactttg tcgagatgtt cgtcggcgtc ggcgcgagcc
    26341 gggtgcgcga cctgttcgag caggggaaga agaatgcccc ctgcatcatt ttcatcgatg
    26401 agatcgatgc ggtcgggcgg caccgcggcg ccggcctcgg cggcgggcac gacgaacggg
    26461 agcagacgct caaccagctg ctggtggaga tggacgggtt cgaatcgaac gagggcgtga
    26521 tcctcatcgc cgccaccaac cggcccgacg tgctcgatcc ggcgctcctt cggccggggc
    26581 gtttcgaccg ccagattgtt gtgccgaacc cggatgtgaa ggggcgggag gggattctcc
    26641 gcgtccatgc gcgcaaggtg aagctttcgg aggaagtgga tttcgaggtg ctggcccgcg
    26701 gcacgccggg gatgtccgga gccgacctgg ccaacgtggt caacgaggcg gccctgctgg
    26761 cctcgcgcaa gaaccgcgac gcggtgacga tggaggattt cgaggaagcc aaggacaagg
    26821 tgctcatggg ctcggcccgg cggtcgctgg tgatctcgga cgaggagaag aaggtgatcg
    26881 cctaccacga atcggggcac acgctggtgg ccaagtatct gccgaaggcc gacccgattc
    26941 acaaagtgac gatcgtcccg cgcggtatgg cgctgggcgt gacccagtcg ctgccggtgg
    27001 acgagcggca cacccactcc aaggagtacc tcgagtcgac gctggcggtg ctcatgggcg
    27061 ggcgggtggc cgagatgatc gtgttcaacc agctcgacac g
//
LOCUS       DAOPOE010000040        27095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2273_length_27095_cov_5.272855, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000040 DAOPOE010000000
VERSION     DAOPOE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27095
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2273_length_27095_cov_5.272855"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKM94_07005"
     CDS             <1..572
                     /locus_tag="PKM94_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOD66427.1"
                     /translation="RARAIRIGNGLDAKTQMGPHINKDQLETSLGYVEIAKGEGAKLL
                     CGGQRVTGGSYDKGWFIQPTIFGEVTQSMRIWREEVFGPVLSIAVCRNFDEAIEMAND
                     TAYGLSASIYTQDINKAYTAMRDVYTGIFYVNAPTTGAEIHLPFGGTKETGNGHREAA
                     TAALDVFTEWKSVFVDFSGVLQRAQIDNA"
     gene            599..727
                     /locus_tag="PKM94_07010"
     CDS             599..727
                     /locus_tag="PKM94_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66428.1"
                     /translation="MRCRYVYHSRHGYLPLYLVEQLWLASSIGRRGGGTRGGHRQS"
     gene            754..2130
                     /locus_tag="PKM94_07015"
     CDS             754..2130
                     /locus_tag="PKM94_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOD66429.1"
                     /translation="MAKTKKRIIIMGAAGRDFHNFNVLYRDNDSVEVVAFTATQIPDI
                     EGRKYPKVLAGKNYPNGIPIHAEDELINLIRKHGADEVVFSYSDVPYHYIMQKAAYVM
                     AAGARFAVEGGAATMLASAKPVVAVTAVRTGCGKSQTTRRVAEVLQALGKKVVAIRHP
                     MPYGDLGKQVVQRFAHLRDLDTHKCTIEEREEYEPHIDRGVVVYAGVDYEKIIRQAEQ
                     EADVILWDGGNNDMSFYKPDLYITVVDPHRPGHEISYYPGQNNLLLADVVVINKIDSA
                     EPEGVAEVRANVSAYNPDAVVIDAASPLDVDKPALIKGKRVLVVEDGPTLTHGEMAYG
                     AGVVAAEKFGAAELVDPRPYTVNSISETFKKYPDIGVLLPAMGYGPKQIKDLETTINR
                     TECDAVVIATPIDLSRIIKIRKPTVRVGYSLQEIGRPNLADVLGEFVGRQKKGAAKAP
                     APRKKARG"
     gene            2127..3152
                     /gene="arcC"
                     /locus_tag="PKM94_07020"
     CDS             2127..3152
                     /gene="arcC"
                     /locus_tag="PKM94_07020"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOD66430.1"
                     /translation="MTTARAGENPRPAGMNCNEDRPIRQTAANRTAVVALGGNAITRP
                     DQEDTIANQFANTRKSLDGIVDLARDGFNLVISHGNGPQVGNALLRVELARGRAPILP
                     LGVLVADTEGGMGYMIEQSLQNRLRSEKIDRRVVTLITQMLIDMNDPALQNPTKFIGQ
                     FYTEEEARRFAKERGWTMKQDAQRGWRRVVPSPKPTAVVNGETIRELVARGIIVIAAG
                     GGGIPVYLDGRGYYEGFDAVIDKDLASAVLANEIGADILCILTAVDHVAVAFGTPQEK
                     KLEEVTVTRMRQYLREGHFPPGSMGPKVEAAVKFIEHGGKLVIITSLENAHAAVHGQA
                     GTRIRPD"
     gene            3154..4551
                     /locus_tag="PKM94_07025"
     CDS             3154..4551
                     /locus_tag="PKM94_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD66431.1"
                     /translation="MNELSREAIEAVLEKGRMYEVGGTVRDRLLAGSPASADRDFLVT
                     GIGYDELSRLLRQFGQVNLVGRSFGVIKYTEYRDGAPVTFDISLPRTEHSTGVGHKDF
                     EVRYDPALAIEDDLVRRDFTINAMAIALGSDEIIDPLGGQEDLRKRLVRMVYPESFTD
                     DPLRMLRAVGFAARFEFDIEERTFAAIAAKAPLIRTVSAERIAEELRKLLERAARPSR
                     GLQLMERSGLLAEVLPELQACVGVAQPGGFHAYDVFEHTLRTIDACPRGLAVRLAALF
                     HDINKPQAKREVIREGQPGATFYGHEMLGAATARSVLRRLRFAHDLIRQVTTLVERHM
                     FTTDVTDKGLRRLVRRVGVPLIFDLLDLRRADVTAQGMGGRTDDVDEFEARIRAELDR
                     KPPFSLNDLALDGGDVMRILKVAPGPLIGRVLDYLLEQVLDDPELNQPETLADLAREF
                     YEATKESPNDKESDL"
     gene            4548..5687
                     /gene="sucC"
                     /locus_tag="PKM94_07030"
     CDS             4548..5687
                     /gene="sucC"
                     /locus_tag="PKM94_07030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOD66432.1"
                     /translation="MKIHEYQAKQLFARHGIPVPAGDVATTPLEAYQIADRVNKPVMV
                     KAQVHVGGRGKAGGVKYAENAEAAKVLATTILGMDIKGLTVKKVLVTEAADIISEAYV
                     GIILDRASRRPVIMVSAAGGIDIEEVAAKTPEKIHKLAVDPVTGLKLFQARNLAYKLY
                     RDISQVRQAADIIMKLYETFWAVDASLVEINPLITNAASEVVAIDAKVNIDDNALYRQ
                     PAITAMRDLDAEPPTETQAREADLSYVKLEGTIGCMVNGAGLAMATMDLVKRYGGEPA
                     NFLDIGGSSNPQKVVTALRIITSDPNVKAILVNIFGGITRCDDVANGLVQALDELKPK
                     TPIVVRLAGTNEAEAARILARYHLPFEDTLDKVVKKAIALAGGQD"
     gene            5700..6554
                     /gene="sucD"
                     /locus_tag="PKM94_07035"
                     /pseudo
     CDS             5700..6554
                     /gene="sucD"
                     /locus_tag="PKM94_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            6826..7092
                     /locus_tag="PKM94_07040"
     CDS             6826..7092
                     /locus_tag="PKM94_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD66433.1"
                     /translation="MTAIETESGETREKPGPQNKYDYSKGPAPLNINLAWCKACNICI
                     ALCPRQVFEPDRDGKPVLARPENCTQCQICWIHCPDFAITSNYK"
     gene            7107..8240
                     /locus_tag="PKM94_07045"
     CDS             7107..8240
                     /locus_tag="PKM94_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOD66434.1"
                     /translation="MPLNQQLLQGNAACVQGALYAGMRFYAGYPITPSTEIAEVSARE
                     LPKLGGRFIQMEDEIASIAAIVGASVSGLKAMTGTSGPGFSLMVENLGYAYMTEVPVV
                     IVNVQRGGPSTGLPTKVSQSDTMQARWGTHGDYTAIAVAPSNVNDTFTETVRAFNLAE
                     RFRTPVTVLLDEVIGHSREIIRIPEPGELEVRDRVRPTVPPEQYVPYEMTANGVNPMP
                     AYGEGYRYNVTGLTHDAKGFPTNRADEIKPKLDKLRFKIEHFVDEIAKLRTEYVEDAE
                     YVYVTYGSVARTALQATKLARQAGFKVGCVQLYTVWPFPDRQLRELCGRARQVIVGEL
                     NMGQIIHEVRRVLPPDIPVHGLQRYDGEILTPMQLLEKLEEVL"
     gene            8240..9106
                     /locus_tag="PKM94_07050"
     CDS             8240..9106
                     /locus_tag="PKM94_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOD66435.1"
                     /translation="MATVTDSKESRKQRSDVTLGYLRPGKKFPNVWCPGCGIGVVMGA
                     FIRALDKLGYGKDEVVMVSGIGCSSRMPVYMDFNSLHTTHGRALPFATGVKFARPEMK
                     VVVITGDGDALAIGGNHFIHACRRNIDITTILINNNIYGMTGGQGSPTTPANAFSTTT
                     PYGNSEKHFDACELAKAAGATWVGRGTVYHTPQLENLITKALEHKGFSLVEAVSNCHT
                     YFGRLNRQGDAVTMLQSFKENAVMASKAKSMTKEELEGKFTIGVLHDDTQTSEFCDEY
                     RMLVERVRAKKK"
     gene            9115..9693
                     /locus_tag="PKM94_07055"
     CDS             9115..9693
                     /locus_tag="PKM94_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD66436.1"
                     /translation="MAHRLLDKIQVPTDRFEMRLSGSGGQGMIFASVVLSKAIGTGEQ
                     AKKVCQSQSYGPEARGGASKSDVVVSSDEIYYPKAMKLDLLLAMTQEALDKYFGDLKG
                     DGILIVDSSLVSEYPADRAYALEFTRMARNDIGSIMVANVIALGSIAALTGLVSCEKL
                     TEAVLQHAPKGTEDKNRRAVELGWEEALKLKK"
     gene            9776..10183
                     /gene="ndk"
                     /locus_tag="PKM94_07060"
     CDS             9776..10183
                     /gene="ndk"
                     /locus_tag="PKM94_07060"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOD66437.1"
                     /translation="MNRTLLIIKPDATERNLIGHVIGRLERAHFRIIAMRMERLTAAE
                     AERFYEVHRERPFFRSLVAFMTSGPVVSMVLEKENAVADLRTLMGATNPAQAACGTIR
                     QEIGVNVEKNSVHGSDSEENAQREIAFFFESPV"
     gene            10287..11513
                     /locus_tag="PKM94_07065"
     CDS             10287..11513
                     /locus_tag="PKM94_07065"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOD66438.1"
                     /translation="MQQVALHYGRGELVLAIPDEYAVTAFAPRPADEPVTYERFRAEF
                     IGRGGLAAIRGEPVVVVNDGFRRTPTALVLEWLDRIDSGLLDRASFLAATGTHGTPSE
                     EDWGKIFGRFRERVRARTAVHDCRDAGSMTRLGEDPLGGEVYVNRAVLGTRPVVVIGS
                     VEPHYFAGYTGGRKGIFPGMVDLATTERNHNLARAMAAAPLRLAGNPVAEHLQALMDR
                     IDGARFFSIQAVLDAAQRVAGVFCGGMTESFQRATVLADQIYAFRGAGPFGTVLAELR
                     PPLDRNLYQLQKAVENCQAAVADGGALVVVSACAEGMGSPHFADLATRWDPGGNAPRD
                     GGPATFGSHKLSRVVAIARRIAVRVHTEMKDDEVRRVFYEPLDNVANFLYLRRDACEN
                     NNLAVVYDAGHTVLTA"
     gene            11547..12473
                     /gene="mdh"
                     /locus_tag="PKM94_07070"
     CDS             11547..12473
                     /gene="mdh"
                     /locus_tag="PKM94_07070"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOD66439.1"
                     /translation="MNRKIAVVGAGNVGASCAMYLAEANLADVAMVDIIEGLPQGKGL
                     DLSQAGPVRGYNAMIDGSNDMAAVKGADIVVMTAGLARKPGMTREDLLTKNAEIVGGV
                     AKEIKKHAPKAYVIVVSNPLDLMTYHMMKVTGFPKERVIGQAGVLDSTRFRTFVAMEL
                     GVAMADVQAMVLGGHGDTMVPLSRYTTVAGIPIGELIPKERIKAIEERTANGGGEIVK
                     LLKTGSAYYAPAAATVQMVRAILNDEKRLLPCSCYLTGEYGIEDMHIGVPCILGAKGV
                     EKILELKLNKAELTSLQKSAATYKEHMKIMGY"
     gene            12485..13729
                     /gene="icd"
                     /locus_tag="PKM94_07075"
     CDS             12485..13729
                     /gene="icd"
                     /locus_tag="PKM94_07075"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOD66440.1"
                     /translation="MAKYKHIVVPSEGTRIVVKNKQLQIPDEPIIPFIEGDGTGRDIW
                     KATRRVVDAAVEKAYGGRKRIVWMEIYAGEKANELYKEWLPDETVDAIRKYVVALKGP
                     LTTPIGGGFRSLNVSLRQFLNLYACVRPVRYFRGVGSPVCHPEKLNVVIFRENTEDVY
                     AGIEWQKGSKEAQKVIAWLNKNMKQAIRTDSGIGIKPISMTGTTRLVRKAIKYALEKK
                     HKSVTLVHKGNIMKYTEGAFRDWGYRVAKKEFGAHTITEDELWSKYDGKQPEGKLLIK
                     DRIADSMFQQILTRPDEYEILATPNLNGDYLSDACAAQVGGLGMAPGANIGDFVALFE
                     ATHGTAPKYADKDMINPGSLILSAAMMLDYLNWPEAGALIHSALQTTIDRKTVTYDLE
                     RQMPGATKVGTAEFGMQIIKNM"
     gene            complement(13817..14236)
                     /locus_tag="PKM94_07080"
     CDS             complement(13817..14236)
                     /locus_tag="PKM94_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD66441.1"
                     /translation="MKSVWLICSCVLLLVAAPLVCTPVGAADEEPADSLSAPTPDDFV
                     AVDRMPEVIHSEQPVYPPEAREKGIEGSLYIRALIDTAGVPSRVKVQKSSGIPALDSS
                     AVDAAGKNRYTPAVYEGKPVSVWISYKVVFALGDKPK"
     gene            complement(14343..14966)
                     /locus_tag="PKM94_07085"
     CDS             complement(14343..14966)
                     /locus_tag="PKM94_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOD66442.1"
                     /translation="MFYRQGVFHVDAAHAVHGRPRGGELRLGPGLESVTDSSIRALSS
                     ATAGSITLVGPMEPGIPPVPPSTITLMTKAGASIGVGTDLPTKALHVVGNICSAGSIG
                     ACSDVRFKNDIEPLDGALPPVDRLTGVRYSWRVEDFAEIHFGTDRQVGLVAQEVRKVL
                     PEAVTEQSDGSLTVDYSRLTPVLIQAIKELRSENESLKERVEAPERT"
     gene            complement(15097..15552)
                     /locus_tag="PKM94_07090"
     CDS             complement(15097..15552)
                     /locus_tag="PKM94_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD66443.1"
                     /translation="MTALRIILLVTLAVLPAGCVRDFFPGRVDRESFSFYAGPCDTPP
                     APADSAFDTRLQPQVIRRPADAYPPLPPWAATAGSVRLRVLVGCDGRVIESVVSTSSG
                     SPILDAAAREGVALCRFAPAVENNRPVASWIRVRYDFLPPRADGARLRR"
     gene            complement(15549..15950)
                     /locus_tag="PKM94_07095"
     CDS             complement(15549..15950)
                     /locus_tag="PKM94_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66444.1"
                     /translation="MWRFAFFAWLPLPVLCVLSGVLRQLPLTPHLGEPAACPLSGLSL
                     SLLIAAYVVLLGRRRRLAPGPTRATGGLWLVLTVAFELLIGYYAAGDSWSRLLANYDI
                     TSGNLWLLDVVWVAAAPRSLLSGRAPAAGSR"
     gene            complement(15972..16616)
                     /locus_tag="PKM94_07100"
     CDS             complement(15972..16616)
                     /locus_tag="PKM94_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOD66445.1"
                     /translation="MASLIDISQPLVAGMAVWPGDAPFRPFWTARLAETGSVNIGGVT
                     MSLHTGTHLDAPKHFRDSGSPVADLSLDPCVGEAVVFDLASARGPLDTEAILPELLAS
                     LKDNFAPRVLLKTGTGGDGRTFPGSFSHLTLPAACFLADLGVRLIGVDTPSVDRVDSR
                     DMGVHHLLADRGIVILEGLVLRHVVPGRYELIALPLKFADMDASPVRAVLRTLD"
     gene            complement(16616..17806)
                     /locus_tag="PKM94_07105"
     CDS             complement(16616..17806)
                     /locus_tag="PKM94_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD66446.1"
                     /translation="MADLLSYRRHFDSLSRCCYLISNSLGAMPNAARSAAAAYADQWA
                     ARGVRAWHDEWWELPRTVGDHIGAFMHAAPDTVAMQPNVTTAEAVILSCFDFAAPRNK
                     IVMVDMEFPSIKYLCQEWAAARGGRVEIVPCPDGVTVPTERLLEAIDEATLLVPISHV
                     LFRSSFIIDAPAVIEKAHRVGARVVLDVYQSLGSIPVDLGALDADFAVGGCLKWLCGG
                     PGAAFLYVRPDLIGSLRPSFTGWLAHDAPFAFEPPPIRRAAGSYRFLNGTPVIPALYT
                     CRPGLDLVSEIGIERIRAHSQAMTARLLELARLRGWKTTAPEDPARRAGTVAIDLDHG
                     LAIATELNARSFLCDYRPRAGIRLSPHFYNTEREIEDVIAAIETIIAEGAYRKHLGED
                     RVVT"
     gene            complement(18147..18587)
                     /locus_tag="PKM94_07110"
     CDS             complement(18147..18587)
                     /locus_tag="PKM94_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD66447.1"
                     /translation="MLVRDLLKTKTRAVITIVPEAVVARAMHLLLANRISCLPVVDSG
                     GMLRGIISDKDIFRAAHDRDRDFKTIPVADLMTTDLIIGVLDDDIEYIAAVMTNNRIR
                     HVPIIDDGRLANLLSIGDILKAQLRDLKVDNRYLRQYIDGAYPG"
     gene            18774..19454
                     /locus_tag="PKM94_07115"
     CDS             18774..19454
                     /locus_tag="PKM94_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66448.1"
                     /translation="MSRQKATINILFALFLAMAGGMRGQAESEDPVLRYYCQRAAGQG
                     ESRDPAKTGLRYEVWVRTYLTHFGRGNKVELTDSALFRRFCSFGAIDSQQTEAVTKES
                     LAKFDLSYPNVFQGAYEFRFFPNDTGGAALAIGFESDTVQNLSPVGLAVIDRTSFYLQ
                     RLYLYYPNAEKYERYSRTFSLRLQDGYVFPDTVVTMYSEAGIFAVEHYRIDTYVDSVR
                     IFRDRPGE"
     gene            19514..20278
                     /locus_tag="PKM94_07120"
     CDS             19514..20278
                     /locus_tag="PKM94_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOD66449.1"
                     /translation="MAADADRLKDQVLARPDRIPRHVAIIMDGNGRWAAKRNKPRTYG
                     HEAGVKAVRATVRAAGELGIRYLTLYTFSSENWQRPRSEVAALMSLLSRTTRRELDEL
                     VKHDVKLITVGRVNGLSASRQRVLARAVEKTRRNKGLTLCLALNYGGRTEILDAVKAI
                     AGAARAGMIDMPEITEELFSSFLYTAGLPDPDLLIRTSGEMRISNFLLWQTSYTELYV
                     IDTLWPDFGRIELFQAVADYQRRERRFGKLSEKTRL"
     gene            20275..21129
                     /locus_tag="PKM94_07125"
     CDS             20275..21129
                     /locus_tag="PKM94_07125"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOD66450.1"
                     /translation="MSRNLALRILVAALFIPAILWIGYAGGWWLYAMLLVFLTVGLGE
                     YLIREGYRLDRPVFWLTEATAVAVYSVVALPRLGVEGLGEFVIYRWHVAAAGLLAAFF
                     LVSGMLFTLGKRPPAELFTRHCRLCWGLAYLSITWPIVFLVGSGVAGQHGGDSLLFLF
                     GLLWVGDTAAMAVGKGLGRHKLSPAVSPNKTVEGFIGGIAGAAAIGVVMYFWKFEGLG
                     LGHALAAAVGASVCGQLGDLVESMWKRSVGVKDSSAIIPGHGGVLDRFDSLLFAAPFL
                     LLYFTLIR"
     gene            21142..22053
                     /locus_tag="PKM94_07130"
     CDS             21142..22053
                     /locus_tag="PKM94_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOD66451.1"
                     /translation="MRWLDYLFAARPLLHVPVWSIYLVSLHYHQMASGREFHGEDLAT
                     PALLSLLCAGAYYLNQVYDREGDALNGKLGFLGPGRLSERGLLGAHLALSVLALAGAA
                     LLSWGLLLVFAQLLVLGYIYSAPPLRLKDRPFWGLAANAWSFGLLIPLTVMRDLSEHT
                     VGLLGGDTPVYFLLAVGGTYLLTTIPDRAGDGATGKRTLAVVWPVGWVKAAALALTGL
                     AAAVAWRGGLAELAGVALISLAPIAVTLLAPSPSSERLAAKAPIAILTLAAGWWFPAY
                     LIFVVVLAAATSVYYRARFNQIYPRLT"
     gene            22053..22568
                     /locus_tag="PKM94_07135"
     CDS             22053..22568
                     /locus_tag="PKM94_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD66452.1"
                     /translation="MRVTAILLLLYTGLLFGCAGYPRYTGGLVERPAEVGERQVDFTT
                     AEYVRLGLIVQSFLGKPYAGASPYDPGIDCSLFIREVFEKYGRIKLPRTAEEQFAAGT
                     QVHRNLLRYGDLVFFRTVPGQVSHVGVYLGNDDFIHASSSRGVIISSLREEYWARSYA
                     GARRVLDSPKG"
     gene            22599..24449
                     /gene="uvrC"
                     /locus_tag="PKM94_07140"
     CDS             22599..24449
                     /gene="uvrC"
                     /locus_tag="PKM94_07140"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOD66453.1"
                     /translation="MAPHIAELDLKLRNLPSSPGVYLFRDRRGRILYIGKAKILRNRV
                     RSYFQRGNGHEERIARMVAQVADLEVIVTDSEVEALIFEANLVREHKPHYNVNLKDDK
                     HFPYIKITTNEPFPRVLIVRRIEKDGATYFGPYTSAKGMRRTVAFLTQLFKIRSCSYV
                     IPPPEGKTLRVCLDYHIKRCGGPCEGLVDQANYRRGVEAVIMVLRGKSRSLTEQLTAR
                     MHEASAAERFEEAAEARDQIEAIESVMIKQKVDVGEPVDRDIVAVAREGGDAVGVVMQ
                     IREGVLIGRQDFQLTAGADRDDQAVLATFLMHYYNHQPNLPDEVHLPFAMEDLDLFAE
                     WLRRQKGAKVKVQAPQIGEKSKLVELTATNARLLLDELLIQKRQVADRTSRMVTGLKD
                     ALKLAVSPREIVCFDISNTGETDAVGSAVYFSNGKPYKSEYRHFRIKGVKGQDDFKMM
                     REVVGRYFHRRTEEQKRMPDLVVVDGGKGQLSAARKELQRAGLAAQPVIGLAKRLEEI
                     YVPGAADPIVIPRSSPALLLLKHVRDEAHRFAVEYNRNVRTKRTIKSVLGEIRGIGPA
                     RQQALLREFGSVEQIRQQTAERLAEVKGITRALAERVLEALREKSGEGGE"
     gene            24643..25872
                     /gene="xseA"
                     /locus_tag="PKM94_07145"
     CDS             24643..25872
                     /gene="xseA"
                     /locus_tag="PKM94_07145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD66454.1"
                     /translation="MQQPKAYTVTAITRMIKNVLEESFQGIWVEGEISNYIHHTSGHR
                     YFSLKDDNAVLKGVMWRSLGNGLKFEPKNGQKVLAFGDIAVYEKGGQYQLNCRKIVPV
                     GIGPLELAYRQLYEKLSAEGLFAAERKQPLPRFAQKIGIVTSPTGAAIRDIVQIARRR
                     NRSVELILYPAQVQGEGAEATIAAGIRYFNTRADIDVLIVGRGGGSLEDLWPFNTETT
                     VRAIAGSRIPVISAVGHEIDFTLADYAADMRAPTPSAAAELAVWPAEEMRRLVRQLVV
                     RQATLLGGQVREARRRLAGSLSRPVLARPMEMVFHRQQRLDHAAERLATAGKNSFEKY
                     RNRLSLVVTRMDALSPLAILARGYSVTRRLPGQTIVRSAGETAPGDRLETLVVDGEII
                     SVVQECRRKQDDEQEEA"
     gene            25850..26158
                     /gene="xseB"
                     /locus_tag="PKM94_07150"
     CDS             25850..26158
                     /gene="xseB"
                     /locus_tag="PKM94_07150"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOD66455.1"
                     /translation="MTNKKKPKDFESALERLEAITEQLEAGESSLEESIRLYTEGLEI
                     ARFCDRKLGEAEKRIKMIQESDGAAVEVETEADAADDDGEEDDEDEEGGDGTGWPSGR
                     "
     gene            26140..27024
                     /locus_tag="PKM94_07155"
     CDS             26140..27024
                     /locus_tag="PKM94_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD66456.1"
                     /translation="MAIGTVSIGAYLREQSAVVEALLDQYLPPASAPPQRLHEAMRYS
                     VLAPGKRLRPILARLSFEYCGGDASGTDKPIHCAMAALEMVHTYSLIHDDLPCMDDDV
                     LRRGMPTCHVKFGEATAVLAGDALHDVAFELLARTGEIQTVAELAEALGTAGMIGGQM
                     ADVEAEGRPVGRGDVMRIHTRKTGALIRCAARLGGILAGAGEPTMARLTRYGENVGLA
                     FQIIDDILDVEGDRELLGKNVGSDSKKLKATYPGAVGLEAAHRDAAALVHEAAALFAA
                     ESDSRLSELALYIGQRNR"
BASE COUNT         5044 a         7970 c         9868 g         4213 t
ORIGIN      
        1 agcgggcccg ggcgatccgg atcggcaacg ggctcgacgc gaagacgcag atgggtccgc
       61 acataaacaa ggaccagttg gagacttcgc tcggctacgt ggagatcgcc aagggggagg
      121 gcgcgaagct cctgtgcggc gggcagcggg tgacgggggg ctcgtacgac aagggatggt
      181 tcattcagcc caccattttc ggcgaggtga cgcagtcgat gcggatctgg cgggaggagg
      241 tgttcgggcc ggtgctgtcg atcgccgtgt gccgcaattt tgacgaggcg atcgagatgg
      301 ccaacgacac cgcctacggg ctgtcggcct cgatttacac gcaggatatc aacaaggcgt
      361 acacggcgat gcgcgacgtg tacacgggga ttttctatgt caatgcgccg acgaccgggg
      421 ccgagatcca cctgccgttc ggcgggacga aagagaccgg caacggccac cgcgaggcgg
      481 ccacggcggc gctggacgtg ttcacggagt ggaagtcggt gtttgtcgat ttctcgggcg
      541 tgttgcagcg cgcgcagatc gacaacgcct gagacatgcg ggcgaaagga aagtccaggt
      601 gcggtgcaga tacgtttacc atagccggca cgggtacctc ccgctgtacc tggtggagca
      661 actgtggctg gcctcgtcga tcgggcggcg gggcggcggg acgaggggcg gtcacaggca
      721 atcgtgaaca ggtgcgctaa ccggaggtag gacatggcca agacgaagaa acggatcatt
      781 atcatggggg cggcgggacg agattttcac aatttcaacg tcctctaccg cgacaacgac
      841 agcgtcgaag tggtcgcatt cacggccacg cagatccccg acatcgaggg gcggaagtac
      901 ccgaaggtgc tcgcgggcaa gaactacccg aacgggatcc ccattcacgc cgaggacgag
      961 ctgatcaacc tgatccgcaa gcacggcgcc gacgaggttg tgttctccta ctcggacgtc
     1021 ccctaccact atatcatgca gaaagcggcc tacgtgatgg cggcgggggc gcgcttcgca
     1081 gtcgaagggg gcgcggcgac catgctggcc tcggccaagc cggtggtggc ggtgacggcg
     1141 gtgcggaccg gctgcgggaa gtcgcagacg acccggcggg tggccgaggt gctgcaggcg
     1201 ctcggcaaga aagtggtggc gatccgccac cccatgccct acggcgacct gggcaaacag
     1261 gtggtccagc gcttcgccca cctgcgcgat ctcgatacgc acaagtgcac gatcgaggag
     1321 cgggaggaat acgagcccca catcgaccgg ggcgtggtgg tctacgccgg cgtcgattac
     1381 gagaagatca ttcgccaggc cgagcaggaa gccgacgtga tcctgtggga cggcgggaac
     1441 aacgacatgt cgttctacaa gccggacctg tacatcacgg tggtcgaccc gcaccgcccg
     1501 ggccacgaga tctcctacta ccccggccag aacaacctcc tgctggcgga cgtggtcgtg
     1561 atcaataaga tcgactcggc cgagccggaa ggggtggccg aggtgcgggc caacgtcagt
     1621 gcctacaatc cggacgcggt cgtgatcgac gcggcctcgc cgctcgatgt cgacaagccc
     1681 gcgctgatca agggcaagcg ggtgctcgtg gtcgaggacg ggccgacact cacccacggc
     1741 gagatggcct acggggcggg cgtggtggcg gccgagaaat tcggcgcggc cgaactggtc
     1801 gatccccggc cctacacggt caactcgatc tcggagacat tcaagaagta cccggacatc
     1861 ggagtcctcc tcccggccat gggctacggc ccgaagcaga tcaaggatct ggagacgacg
     1921 atcaaccgga cggagtgcga cgcggtggtg atcgccacgc cgatcgacct gtcgcggatc
     1981 atcaagatca ggaaaccgac ggtgcgggtg ggttacagcc tgcaggagat cgggcggccg
     2041 aacctggcgg acgtgctggg cgaatttgtc ggacggcaga agaaaggggc ggccaaggcg
     2101 ccggccccga ggaagaaagc ccgcggatga ccacggcgcg ggccggggaa aacccccggc
     2161 ccgccggcat gaactgcaac gaggacagac caatcaggca gacagcagca aacagaacgg
     2221 cagtggtggc gctcggcggg aacgcgatca cccggccgga ccaggaagac acgatcgcca
     2281 accagttcgc gaacacgcgg aaatcgctgg acgggatcgt ggatctggcg cgcgacggtt
     2341 tcaacctggt gatctcgcac ggcaacgggc cgcaggtcgg caacgcgctc ttgcgggtgg
     2401 aactcgcccg cggacgggcg ccgatcctgc ccttgggcgt gctggtggcg gacaccgagg
     2461 gcgggatggg ctacatgatc gagcagtcgc tccagaaccg gctgcgcagc gagaaaatcg
     2521 accggcgcgt ggtcaccctc atcacccaga tgctcatcga tatgaacgac ccggccctcc
     2581 agaacccgac caaattcatc gggcagttct acaccgaaga ggaggcgcgg cgcttcgcca
     2641 aggagcgcgg gtggaccatg aagcaggatg ctcagcgggg gtggcggcgc gtggtcccgt
     2701 cgccgaaacc gaccgccgtg gtcaacggcg agacgatcag ggagctggtc gcccgcggta
     2761 tcatcgtcat cgccgccggc ggcggcggca tcccggtcta cctcgacggc cggggatact
     2821 acgaggggtt cgatgcggtg atcgacaaag acctcgcctc ggcggtgctg gccaacgaga
     2881 tcggcgccga catcctgtgc atcctcaccg ccgtcgacca cgtcgccgtc gctttcggca
     2941 ccccgcagga gaaaaagctc gaagaagtga cggtgacgcg gatgcggcag taccttcgcg
     3001 agggacactt ccccccgggg tcgatggggc cgaaggtgga agcggcggtg aaattcatcg
     3061 agcacggggg aaaactcgtg atcattacgt cgctggaaaa cgctcacgcc gcggtgcacg
     3121 gccaggcggg aacgcgcata cgcccggatt gaaatgaacg aattgtcacg ggaagcgatc
     3181 gaggcagttc tggagaaagg ccgcatgtac gaggtcggag gaacggtccg ggaccggctg
     3241 ctggccggga gtccggcgag cgcggaccgg gatttcctcg tcacggggat cgggtacgac
     3301 gagttgtcgc gcctgctgcg gcagttcggc caggtaaacc tggtgggccg cagtttcggg
     3361 gtaatcaagt acacggagta ccgggacggg gcgccggtga cgttcgacat ttcgctgccc
     3421 cggacggagc actcgacggg ggtggggcac aaggatttcg aggtgcggta cgatcccgcg
     3481 ctggcgatcg aagacgacct cgtgcggcgg gatttcacga tcaacgcgat ggcgatcgcg
     3541 ctggggtccg acgagatcat cgacccgctc ggcgggcagg aagacctgcg caagcggctg
     3601 gtccggatgg tctacccgga gtcattcacc gacgacccgc tgcgcatgct gcgggcggtg
     3661 gggtttgcgg cgcgcttcga attcgacatc gaggagcgga cgtttgcggc gatcgcggcc
     3721 aaggccccgc ttatccggac ggtctcggcc gagcggatcg ccgaggagct gcgcaaactg
     3781 ctcgagcggg cggcccgccc ctcgcgcgga ctccagctga tggaacggag cgggctcctg
     3841 gcggaggtcc tccccgaact gcaggcctgt gtcggcgtgg cgcagccggg cgggttccac
     3901 gcctacgacg tgttcgaaca cacgctgcgg acgatcgacg cctgcccgcg cgggctggcg
     3961 gtgcggctgg cggcgctgtt ccacgatatc aacaagccgc aggccaagcg cgaggtgatc
     4021 cgggaggggc agccgggggc gacgttctac ggccacgaga tgctcggcgc ggcgaccgcc
     4081 cggagcgtgc tgcgccgcct gcgcttcgcc cacgacctga tccggcaggt gaccaccctg
     4141 gtcgagcggc acatgttcac gacggacgtg accgacaagg ggttgcgccg gctggtccgc
     4201 cgggtaggcg tgccccttat tttcgacctt ttggacctgc ggcgggccga tgtgaccgcc
     4261 cagggcatgg ggggacggac ggacgatgtg gacgaatttg aggcccggat ccgcgcggag
     4321 ctggaccgga aaccgccgtt ttcgctgaac gatctggcac ttgacggcgg cgacgtgatg
     4381 cgtatcctga aggtggcgcc cgggccgctg atcggccgcg tgctggacta cctgctggag
     4441 caggtgctcg acgacccgga gctgaatcag ccggagacgc tggcggacct ggcccgggag
     4501 ttctacgagg caacgaagga gagtccgaac gataaggaat ccgacctatg aagattcacg
     4561 agtatcaggc gaagcaactg ttcgcgcgcc acgggatccc ggtgccggcg ggggacgtgg
     4621 cgacgacgcc gctggaggcg tatcagatcg ccgaccgggt gaacaagccg gtgatggtga
     4681 aggcgcaggt ccacgtcggc ggccgcggca aggccggggg ggtcaaatac gccgaaaacg
     4741 ccgaggccgc gaaagtgctc gccaccacca tcctggggat ggacatcaag ggtctgacgg
     4801 tcaagaaggt gctggttacg gaggccgccg acatcatctc ggaagcgtac gtgggaatca
     4861 tcctcgaccg ggcgagccgg aggccggtga tcatggtgtc ggcggcgggc gggatcgaca
     4921 tcgaggaagt ggcggcgaag acgcccgaga agattcacaa gctggcggtc gatccggtca
     4981 ccgggctgaa gctgttccag gcgcggaacc tcgcctacaa gctctaccgg gacatcagcc
     5041 aggtccgcca ggcggccgac atcatcatga agctctacga gacgttctgg gcggtggatg
     5101 cgtcgctggt ggaaatcaat ccgctcatca cgaacgcggc gagcgaggtg gtggcgatcg
     5161 acgcgaaagt caacatcgac gacaacgccc tctaccgcca gccggcaatc acggccatgc
     5221 gggacctgga cgcggagccg ccgaccgaga cccaggcgcg cgaggccgac ctctcgtacg
     5281 tgaaactcga ggggacgatc gggtgcatgg tcaacggagc ggggctggcg atggcgacca
     5341 tggatctcgt gaagcgctac gggggcgagc cggccaattt cctcgatatc ggcggctcgt
     5401 ccaacccgca gaaggtggtg acggcgctgc gcatcatcac ctcggatccc aacgtgaagg
     5461 ccatcctggt caacatcttc ggggggatca cgcggtgcga cgacgtggcc aacggactcg
     5521 tgcaggcgct cgacgagttg aagccgaaga cgccgatcgt agtgcggctg gccggaacga
     5581 acgaggcgga ggcggcgcgc atcctcgcgc gctaccatct gccgtttgaa gatacgctcg
     5641 acaaggtggt caaaaaagcg attgcgctgg cgggcggaca ggactaggcg aggtagcgta
     5701 tggcgatatt catcaatcag aaatccagag tgctcgtgca gggaatcacc ggccgcgacg
     5761 gctcgtttca cgcccagcag atgaaggctt acggcacgca ggtggtgggc ggcgtgacgc
     5821 ccggaaaggg cggctcccag gtggcgggaa tacccgtgtt caacacggtg gaagaggcgg
     5881 tcggggcgac cggggcggac gtgtcggtcg tctacgtgcc gccggcgttc gccgtcgatg
     5941 cgatctacga ggcggtcgac gccgggatcg ggctggtggt gtgcatcacc gaaggacttc
     6001 cggcgaacga catgatgaag atgtacccgt acgtcaaggc gcgcggggcg cggctgatcg
     6061 ggcccaactg ccccggcctc atctcggtcg accagtgcaa gatcgggatc atgccggcga
     6121 cgattgtgaa aaagggcaac gtcggagtgg tctcgcggtc ggggacgctc acctacgagg
     6181 cgatctgggc gctcacgctg gcgggaatgg ggcagaccac ctgcatcgga atcggcgggg
     6241 accaggtgat cgggaccact ttcatcgact gcctggaggc gttcgaggcc gacccggcga
     6301 cgcggggggt ggtcatgatc ggggagatcg gcggatccga cgaggaggag gcggccgcgt
     6361 tcatcaagaa gcgcatgacc aagccggtgg tagccttcat cgccgggcgt tcggcgccgc
     6421 cgggcaagcg gatggggcac gccggggcga tcatctccgg cggcagcggc acggccgagg
     6481 ggaaggtggc ggcgctcacg gcggcggggg tgccggtggc gggatcgccg accgagatcc
     6541 ccgcgctgtt gagagacctg atggccaggc gcgcgggcaa ggccaggagc gcggcgcggc
     6601 cggcggccaa accggcgtcc cgcgcgggga ccaagatcag gaccacatcc ggccgagcaa
     6661 gcgcggcgcg cgcggcccga ccggcggcaa aagcaaacaa aaagccgcac gcggcgaaga
     6721 agaccgggac caaggccagg aagaaagctc cggcgggcag acgaaaatga gcggcgtcgg
     6781 cggacgttcg gccgccacgg cggccgacaa cctcggagac gtgagatgac ggcgatagag
     6841 accgagtcgg gcgagaccag agagaagccc ggcccgcaga acaagtacga ctattcgaag
     6901 gggcccgccc ctttgaacat caacctcgcg tggtgcaagg cctgcaacat ctgcatcgcc
     6961 ctgtgcccgc gtcaggtatt cgaaccggac cgcgacggca agccggtcct cgcgcggccg
     7021 gagaactgca cccagtgcca gatttgctgg attcactgtc cggatttcgc gataacctcg
     7081 aactacaagt gatgggagcg tcgcttatgc cactcaatca acaactgctg caggggaacg
     7141 ccgcgtgcgt gcagggcgcc ctgtacgcgg ggatgcgatt ctacgccggc taccccatca
     7201 cgccctcgac cgagatcgcc gaggtgagcg cgcgcgaact gcccaaattg ggcggccggt
     7261 tcatccagat ggaggatgag atcgcctcga tcgcggccat cgtgggagcc tcggtgtcgg
     7321 ggctcaaagc gatgacgggg acctcggggc ccgggttctc cctcatggtg gagaacctcg
     7381 ggtatgccta catgaccgag gtgccggtcg tgattgtcaa cgtccagcgc ggcggaccct
     7441 cgacgggact gccgaccaag gtgagccagt cggacacgat gcaggcgcgc tgggggacgc
     7501 acggcgacta caccgcgatc gccgtcgcgc cctcgaacgt caacgatacc ttcaccgaga
     7561 ccgtccgggc attcaacctg gccgagcgct tccgcacgcc ggtcacggtg ctgctcgacg
     7621 aggtgatcgg gcactcgcgg gagatcatcc ggatcccgga gccgggggag ctcgaagtgc
     7681 gcgaccgggt gaggccgacg gtgccgccgg agcagtatgt gccgtatgag atgacggcca
     7741 acggggtgaa cccgatgccg gcgtacggcg agggataccg ctacaacgtg accggactca
     7801 cccacgacgc gaaggggttt ccgaccaacc gggcggacga gatcaagccc aagctcgaca
     7861 agctgcggtt caagatcgag cactttgtgg acgagatcgc gaagttgcgg accgagtatg
     7921 tcgaggacgc cgagtacgtg tacgtgacct acggatcagt ggcgcggacg gccctgcagg
     7981 cgacgaagct ggcgcgccag gcggggttca aggtagggtg cgtacagctc tacacggtgt
     8041 ggccgttccc cgaccgccag ctgcgcgagc tgtgcgggcg ggcgcggcag gtgattgtcg
     8101 gcgagctgaa catgggacag atcattcacg aagtcaggcg ggtgctgccg ccggatattc
     8161 cggtgcacgg attacagcgg tatgacgggg aaatcctgac gccaatgcag cttctggaga
     8221 agctggagga ggtgctctaa tggccactgt gaccgacagc aaagagagcc ggaagcagcg
     8281 gtcggacgtg accctgggct accttcggcc ggggaagaaa ttcccgaacg tgtggtgtcc
     8341 gggctgcggg atcggcgtgg tgatgggggc gttcatccgc gccctcgaca agctcgggta
     8401 cggcaaggac gaggtggtca tggtctcggg gatcggctgt tcgagccgga tgccggtgta
     8461 catggatttc aactcgctcc acacgaccca cggacgggcg ctgccgtttg cgaccggtgt
     8521 gaagttcgcg cggccggaga tgaaagtggt cgtgatcacg ggcgacggcg acgcgctggc
     8581 gatcggcggg aatcacttca ttcacgcctg ccggcgcaac atcgacatca ccaccatcct
     8641 catcaacaac aacatctacg gcatgaccgg gggacaggga tcgccgacga caccggccaa
     8701 cgcgttttcg acaaccaccc cctacgggaa ctccgagaag cacttcgacg cgtgcgagct
     8761 ggccaaggcg gcgggggcga cctgggtcgg gcgggggacg gtctaccaca cgccccagtt
     8821 ggagaacctg atcaccaagg cgctcgaaca caagggcttt tcgctggtgg aggcggtgtc
     8881 caactgccac acctatttcg ggcggctcaa ccggcagggg gacgcggtga cgatgctcca
     8941 gtcgttcaag gaaaacgcgg tcatggcgag caaggccaag tcgatgacca aggaggaact
     9001 ggaggggaag ttcacaatcg gcgtgctcca cgacgacacc cagacgagcg agttctgcga
     9061 cgagtaccgg atgctggtcg agcgcgtccg ggccaagaag aaatgaggcg cactatggca
     9121 catcggttac tcgacaagat acaggttccg accgaccggt tcgagatgcg gctgtcgggg
     9181 tcgggcggcc agggaatgat attcgcctcg gtcgtgctgt cgaaggcgat cgggaccggc
     9241 gagcaggcga agaaagtctg ccagagccag tcgtacggtc cggaggcgcg cggcggggca
     9301 tcgaagtccg acgtggtggt gtcctcggac gagatctact acccgaaggc gatgaagctt
     9361 gacctgctgc tggcgatgac gcaggaggcc ctggacaagt acttcgggga tctcaagggc
     9421 gacgggatcc tgatcgtgga ttcctcgctg gtcagcgaat acccggcgga ccgggcctac
     9481 gcgctggagt tcacacgcat ggcgcggaac gacatcggga gcatcatggt ggccaacgtg
     9541 atcgcgctgg gatcgatcgc cgccctcacc gggttggtct cgtgtgagaa gctgaccgag
     9601 gcggtgctgc agcacgcgcc caaggggacc gaggacaaga accgccgggc cgtcgagctc
     9661 gggtgggagg aggcgctgaa gctgaagaag taggggggcg aaccggaccg gatcgacccc
     9721 cgacccgcgg gagcgcgggg ggcgggccgc gcacgaagca gacaaaggag tcggattgaa
     9781 caggacgctg ctgataatca aaccggatgc gacggagcgg aatctgatcg ggcatgtgat
     9841 cggccggctc gaacgggcgc atttcaggat catcgccatg cgcatggagc ggctgaccgc
     9901 cgcagaggcg gaacggttct acgaggtgca ccgggagcgc ccgttcttcc gttcgctcgt
     9961 ggctttcatg acgtccggcc cggtggtgtc gatggtgctg gagaaggaga atgccgtggc
    10021 cgatctccgc acgctcatgg gggcgaccaa cccggcccag gcggcgtgcg ggacgattcg
    10081 gcaggagatc ggcgtgaacg tggagaagaa ctcggtgcac ggctccgact ccgaggagaa
    10141 tgcgcagcgc gagatcgcgt tcttctttga aagtccggtg taaacagcgg cgcgcgggcg
    10201 gcccggccgc ccgccgcccg acggtgtcat gacgggccgg atcccccgcc gggagccggc
    10261 ctgtcgcatt ggggaagtcc ttgcgcatgc agcaggtggc gcttcactac gggcggggag
    10321 aactggtcct cgccattccc gacgagtacg cggtcaccgc cttcgccccc cggccggcgg
    10381 acgagccggt cacctacgag cggttccgcg cggagttcat cggacggggc gggcttgcgg
    10441 cgatccgggg agagccggtc gtggtggtca acgacggttt tcgccgcacg cccacggcgc
    10501 tcgtgctcga gtggctcgac cggatcgact ccggactcct ggaccgggcg tcatttctgg
    10561 cggccacggg aacgcacggg acgccgagcg aggaggactg ggggaagatt ttcggccgct
    10621 tccgggagcg ggtgcgggcg cggacggccg tccacgactg ccgcgacgcc ggatcgatga
    10681 cgcggctggg agaggacccg ctgggcggcg aggtctatgt caatcgggcg gtgctcggga
    10741 cgcggccggt ggtggtgatc ggatcggtcg agccgcacta tttcgcgggc tacaccgggg
    10801 ggcgcaaggg gattttcccc ggcatggtcg acctggcgac gacggagcgg aaccacaatt
    10861 tggcgcgggc gatggcggcg gcgccgcttc gtctggccgg gaacccggtg gccgagcatt
    10921 tgcaggcgct catggaccgg atcgacgggg cgaggttttt ctcgattcag gcggtgctcg
    10981 atgcggcgca gcgggttgcg ggcgtcttct gcggaggaat gacggagtcg ttccagcggg
    11041 cgacggtcct ggccgaccaa atctacgctt tccgcggggc ggggccgttc gggacggtgc
    11101 tggcggaact ccgtccgccc ctggaccgga acctgtacca gcttcagaaa gcagtcgaga
    11161 actgtcaggc ggcggtggcc gacggggggg cgctggtcgt ggtctcggcc tgcgcggagg
    11221 ggatggggtc gccgcatttt gccgatttgg cgacgcgctg ggatccgggc ggcaatgccc
    11281 cgcgcgacgg cggcccggcc actttcgggt cgcacaagct gtcgcgggtc gtggcgatcg
    11341 cccggcgcat tgcggtgcgc gtccacacgg agatgaaaga tgacgaagtg aggcgggtgt
    11401 tttacgaacc gcttgacaac gtggcgaact ttttgtactt aaggcgcgac gcatgtgaaa
    11461 ataataactt agcggttgta tatgacgcgg gccacacggt tctcactgca taacgctccg
    11521 cgccgtggca acaccaggag gttcgaatga acagaaagat cgccgtagta ggagccggga
    11581 atgtgggtgc gtcgtgcgcg atgtacctgg ccgaggcgaa cctggccgat gtcgcgatgg
    11641 tcgacatcat tgaggggctg ccccagggga aggggctcga cctgagccag gccgggccgg
    11701 tgcgcgggta caacgccatg atcgacgggt cgaacgacat ggcggctgtc aagggcgcgg
    11761 acattgtagt gatgacggcc gggctggccc gcaagccggg catgacgcgc gaggatctgc
    11821 tgaccaagaa cgccgagatc gtgggcggcg tggccaagga gatcaagaag cacgcgccca
    11881 aagcctacgt gatcgtggtc tccaacccgc tcgacctgat gacctaccac atgatgaaag
    11941 tgacgggctt ccccaaggag cgtgtcatcg gccaggccgg cgtgctggat tcgacacgct
    12001 tccgcacatt cgtggccatg gagctggggg tggcgatggc cgacgtgcag gcgatggtgc
    12061 tcgggggaca cggcgacacg atggtgccgc tgtcgcgcta cacgaccgtg gcgggcatcc
    12121 cgatcggcga gctgatcccg aaagagcgga tcaaggcgat cgaggaacgg acggccaacg
    12181 ggggcggcga gatcgtcaag ctgctcaaaa ccgggtcggc ctactacgca ccggcggcag
    12241 cgaccgtgca gatggtgcgg gcgatcctca acgatgagaa gcgtctcctg ccgtgctcgt
    12301 gctacctcac gggcgagtac gggatcgagg acatgcacat cggcgtgccg tgcattctcg
    12361 gcgccaaggg tgtggagaag atcctcgagc tgaagttgaa caaggcggaa ctgacgtcgc
    12421 tgcagaagtc ggcggccact tacaaagaac acatgaagat catgggatac tagaggagcg
    12481 agcgatggcg aagtacaagc atatcgtcgt cccgagcgag gggacgcgca tcgtcgtcaa
    12541 gaacaaacag ctccagatcc ccgatgagcc gatcatcccg tttatcgagg gagacggcac
    12601 cgggcgggat atctggaaag cgacgcggcg ggtcgtcgat gcggcggttg agaaggccta
    12661 cggcggcagg aagcggatcg tgtggatgga gatctacgcg ggggagaagg ccaacgagct
    12721 gtacaaggag tggctgccgg acgagaccgt ggatgcgatc cgcaaatacg tggtggcgct
    12781 caaggggccg ctcaccacgc cgatcggggg cgggttccgc tcgctcaacg tgagcctgcg
    12841 ccagttcctc aacctctacg cctgcgtccg gccggtgcgc tatttccgcg gcgtcgggtc
    12901 acccgtctgc cacccggaga agctcaacgt cgtcatcttc cgcgaaaaca cggaagacgt
    12961 ctacgccggg atcgagtggc agaaggggtc gaaggaggcg cagaaagtga tcgcctggct
    13021 gaacaagaac atgaagcagg cgatccggac cgacagcggg atcggaatca agccgatctc
    13081 gatgaccggg accacccggc tggtgcgcaa agcgatcaag tacgcgctgg agaagaagca
    13141 caaatcggtg actttggtgc acaaggggaa catcatgaag tacaccgagg gcgcgttccg
    13201 cgactgggga taccgggtgg cgaagaagga gttcggcgcc cacacgatca ccgaggacga
    13261 gctgtggtcg aagtacgacg ggaagcagcc ggagggcaag ctgctgatca aggaccggat
    13321 cgccgattcg atgttccagc agattctcac ccggccggac gagtacgaga tcctggcgac
    13381 gccgaatctg aacggggact acctgtcgga tgcgtgcgcc gcgcaggtcg ggggcctggg
    13441 gatggcgccg ggcgcgaaca tcggcgattt cgtggccctg ttcgaggcga cccacggcac
    13501 ggcgcccaag tacgccgaca aggacatgat caaccccggc tcgctgatcc tgtcggcggc
    13561 gatgatgctg gattacctga actggcccga ggccggtgca ttgattcact cggcgctgca
    13621 gacgacgatc gaccggaaga cggtcaccta cgacctggaa cgccagatgc cgggggcgac
    13681 caaggtcggg acggcggagt tcggaatgca gatcatcaag aacatgtaga tcggaccggc
    13741 cgacacatga aaaggcagga ccgcgagcgt cctgcctttc ttttttggct gagcggcggc
    13801 gaggggcgcg cgccggtcat ttcggcttgt ccccgagggc aaacacgacc ttgtaggaga
    13861 tccagacact cacgggtttg ccttcgtaaa ctgcgggggt gtagcggttc ttgccggcgg
    13921 cgtcgaccgc gctgctgtcg agcgccggga tgcccgacga cttctgcact ttcacccgcg
    13981 aaggaacgcc ggccgtgtcg atgagcgccc ggatatagag ggagccttcg attcccttct
    14041 cccgggcctc cggcgggtac accggctgtt cgctgtggat gacctcgggc atccggtcga
    14101 cggccacgaa atcatcgggc gtcggcgcgg acagactgtc ggcgggttct tcatcggcgg
    14161 cgccgaccgg cgtgcacacc agcggcgcgg cgaccaaaag caggacgcag gaacagatca
    14221 gccagacgct tttcatcgtc tacctccagt aaaatggtct gttgcaaaaa cgcgccgcgg
    14281 ggacaaaacg tgcaatgaga tttcggtctt cgtcgcgggc cggccgcgcc gccccggggc
    14341 ggctacgtcc tctccggcgc ctccacccgt tctttgagcg actcgttttc ggaccggagt
    14401 tccttgatcg cctgtatcag cacgggcgtc aggcggctgt agtcgaccgt cagagatccg
    14461 tccgattgct cggtgacggc ctcggggagc actttccgga cctcctgggc cacgaggccg
    14521 acctggcgat cggtgccgaa atggatctcg gcgaagtcct caacgcgcca ggagtagcgg
    14581 acgccggtga gccggtcgac cggcggcagg gcaccgtcga gcggctcgat gtcattcttg
    14641 aagcggacat cggagcaggc gccgatagag ccggcggagc agatgttgcc gaccacgtgc
    14701 agggccttgg tggggaggtc agtgccgaca ccgatgctcg cgccggcctt ggtcatcaag
    14761 gtaatcgtgc tcggcgggac cgggggaatg cccggctcca tgggacccac gagcgtgatc
    14821 gagccggccg tggcggacga aagggccctg atggaggagt cggtgacgga ctcgaggccc
    14881 ggtccgagac ggagttcgcc gccgcgaggg cggccgtgca cagcatgcgc cgcatcgaca
    14941 tggaagactc cttgccgata gaacacggtt gtttctcgga gcggcaattc cggggttcat
    15001 tctgcgtgtc tgcaatgtcc tggccgaacg gttcctgggc atgttgaaac tggagaacac
    15061 tgtctgaaat ggaatcccgc gcggggcgca ccggggctag cggcggaggc gggcgccgtc
    15121 ggcgcgcggc gggaggaagt catagcgcac gcgaatccag gaggcgaccg ggcggttgtt
    15181 ctcgaccgcc ggggcgaacc ggcagagcgc gacgccctcc cgggcggccg cgtcgagaat
    15241 cggcgatccg ctcgaggtgc tcaccacgct ctcgatgacg cgcccgtcgc agcccaccag
    15301 cacgcggagg cggacgctcc cggcggtcgc ggcccacggc ggcagcggcg ggtaggcgtc
    15361 ggcaggccgg cggatcacct ggggctggag gcgggtgtcg aaagcgctgt cggcgggggc
    15421 gggcggcgtg tcgcacggcc cggcgtagaa gctgaagctc tcccggtcga cgcggccggg
    15481 gaaaaaatcg cggacgcatc cggccgggag aaccgcaagg gtgaccagaa gtatgatgcg
    15541 aagcgctgtc atcgcgatcc ggcggcggga gcccgcccgc tcaaaagcga tcgcggcgcc
    15601 gccgcaaccc ataccacgtc gaggagccag aggttgccgg acgtgatgtc gtagttggcc
    15661 aggagccggc tccaggaatc tcccgccgca taatagccga tgaggagctc gaaagcaacg
    15721 gtgaggacaa gccagaggcc cccggtcgcc cgcgtcgggc cgggcgcaag acgcctgcgg
    15781 cggcccagga gaaccacgta cgctgcgatc agcagagaga gcgagagccc ggacagcggg
    15841 caggccgccg gttccccgag atgcggggtc aacgggagct gccgaagcac gccgctgagc
    15901 acgcagagaa cgggaagggg aagccaggcg aagaacgcga atctccacat tgcggcgccc
    15961 gaccgggacg gtcaatcgag agttcggagg acggcgcgga ccggcgaagc gtccatgtcc
    16021 gcgaatttga gcggcagggc gatcagctcg tagcggccgg ggaccacgtg ccttagcacg
    16081 agaccctcga gaatgacgat gccgcggtcg gcgaggagat ggtgcacgcc catatcacgg
    16141 gagtcgacgc gatcgacgga gggggtgtcg acgccgatga gccgcacgcc gaggtcggcc
    16201 aggaagcagg ccgccgggag agtgaggtgg gagaacgacc ccggaaaggt ccgaccgtcg
    16261 ccgccggtcc cggtcttcag cagcacccgc ggggcgaagt tatctttcag actggcgagg
    16321 agttcgggga gaatcgcctc ggtgtcgagc ggcccgcggg cggatgcaag gtcgaagacg
    16381 acagcctcgc cgacgcacgg gtcgagactc aggtcggcca caggggaccc cgagtcgcgg
    16441 aaatgcttgg gggcgtcgag gtgggtgccg gtgtgaaggc tcatggttac gccgccgatg
    16501 ttgaccgagc cggtctcggc cagccgcgcg gtccagaagg gccggaaagg ggcatcgccc
    16561 ggccagaccg ccatgccggc gacgagcggc tgcgagatat cgatcagact tgccatcagg
    16621 taaccacgcg gtcttccccg aggtgctttc ggtaggcgcc ctcggcgatg atggtttcga
    16681 tcgcggcgat cacgtcctcg atctcccgtt cggtgttgta gaagtgcggc gagaggcgga
    16741 tgccggcccg ggggcggtaa tcgcacagga agctgcgggc gttgagctcg gtggcgatcg
    16801 ccagaccgtg gtcgagatcg atggcgaccg tgccggcgcg gcgcgcggga tcctccggag
    16861 cggtggtttt ccagccgcgg aggcgggcca gctcgagaag gcgggcggtc atcgcctgcg
    16921 agtgcgcgcg gatgcgttcg atgccgatct ccgagacgag atcgaggccg ggacggcacg
    16981 tgtagagagc cggaatgacc ggggtgccgt tcaggaagcg gtaggagccc gcggcgcggc
    17041 ggatcggcgg cggctcaaag gcaaacgggg catcgtgggc cagccagccg gtgaagctcg
    17101 ggcggaggga gccgatgagg tcgggacgga catagaggaa cgcggcgccg ggaccgccgc
    17161 acagccattt gaggcagccg ccaacggcga agtcggcatc aagggcgccg aggtcgaccg
    17221 gaatcgaacc gagcgactgg taaacgtcga gaaccacccg ggcgcccacg cgatgggcct
    17281 tctcgatgac ggccggggca tcgatgatga acgagctgcg gaaaaggacg tgcgagatgg
    17341 gaaccagcag cgtggcctcg tcaatggcct cgaggaggcg ctcggtcggg accgtgacgc
    17401 cgtcggggca gggcacgatc tcgacacggc cgccgcgggc agcggcccac tcctggcaga
    17461 gatacttgat cgaggggaat tccatgtcga ccatgacgat cttgttacgg ggggcggcga
    17521 agtcgaagca ggagaggatg acagcctcgg cggtggtgac gttcggctgc atggcgacgg
    17581 tgtcgggggc ggcgtgcatg aaagcgccga tgtgatcgcc gacagtgcgg ggcaactccc
    17641 accactcgtc gtgccaggcc cgcacccccc gggcggccca ttggtcggcg taggcggcgg
    17701 ccgcggagcg ggcggcgttg ggcatggctc cgagagagtt ggagatgagg tagcagcaac
    17761 ggctcaggga atcgaaatgc cggcgatagc tcagaagatc ggccatgaga tgtttcctcg
    17821 gggcgcccgg gcccccgtac atcggttgag cctatagtac gagggccggg gcggcgggga
    17881 caaacggtat tttcggggcc gggtggaaaa agtgggaagt tttagtcgac agcggtggcc
    17941 ggagccggct ttcgtagaat atcagcaccg gcatcacgcg atcggcggtg ttgctatgcg
    18001 aaatggcgcc ggcggacatc tagtccgccg gtttctcttt ccaaccggcc aaccggagaa
    18061 gagacgatgg atttcctgct gctgccggcg gtgacgccgg ccgtcgcagt cgtcgtcacg
    18121 gcggccggtc gcgcgcccgc ccgccctcag ccgggatacg cgccgtcgat gtactgccgg
    18181 aggtagcggt tgtcgacttt caggtcgcgc aactgcgctt tcaggatgtc cccgatcgaa
    18241 aggaggttcg ccagccggcc atcgtcgatg atcggcacgt ggcggatgcg gttgttggtc
    18301 atgacggccg cgatgtactc aatgtcgtcg tcgaggacgc cgatgatcag gtcggtcgtc
    18361 atgaggtcgg cgaccgggat ggtcttgaag tcgcggtcgc ggtcatgggc cgcccggaag
    18421 atgtctttgt cgctgatgat gccccggagc attcccccgg agtcgacgac cggcaggcaa
    18481 ctgatgcggt tggccagcag gaggtgcatg gcgcgggcga cgacggcttc gggcacaatc
    18541 gtgatcacgg cccgggtctt ggttttaaga aggtctctga cgagcatgcg taatcctccg
    18601 cggcgaaggg tttcgggacg gctcctttac ggacgtgctc atcggcataa cctctgtcgg
    18661 gtgactgttg tccggccggc gggaaacccg atgaagcggc agatgccggg gcaagggagt
    18721 cggacaaagg attcgttggc aacttgaata caatgtgata tactgagttg gctatgtcaa
    18781 ggcaaaaagc gaccataaat atcctctttg ctctttttct ggccatggcc ggcggtatgc
    18841 gggggcaagc ggaatccgaa gatcccgtgc tgcgctacta ctgccagcgg gcggccgggc
    18901 agggtgaaag ccgcgacccg gccaaaaccg gccttcgcta cgaagtgtgg gtgcggactt
    18961 acctgaccca cttcgggcgg gggaacaagg tggagctgac cgattcggcg ttgtttcggc
    19021 gcttctgcag tttcggcgcg atcgactcgc agcagacgga ggcggtgacg aaggagtcgc
    19081 tggcgaagtt cgacctgtca tacccgaatg tctttcaggg cgcctacgag ttccggttct
    19141 ttccgaacga caccggcggg gcggcgctgg cgatcgggtt cgagagcgac acggtgcaga
    19201 acctgagccc ggtgggattg gcggtgattg accggaccag tttctacctg cagcggctct
    19261 acctctatta cccgaacgcg gagaagtatg agcggtactc gcggacgttc agtctccggc
    19321 tgcaggacgg gtacgtgttt ccggacacgg tggtgacgat gtactccgag gcggggatat
    19381 tcgcggtgga gcattaccgg atcgacacct atgtcgattc ggtgcggatt tttcgggacc
    19441 ggccgggcga gtgaacctcg cgggcggcga aagggcttgc ccggcaggcg ggagcaggat
    19501 tactttgcgc cgtatggcag cggacgcgga tcgactcaag gaccaggtac tggcgcggcc
    19561 ggaccgcatc ccccggcacg tggcgatcat catggacggc aacggtcgct gggcggccaa
    19621 gcgcaacaag ccgcggacct acgggcatga ggcgggggtc aaggcggtgc gggcgacggt
    19681 gcgggcggcc ggggaactgg gaatccgcta cctcaccctc tacacgttct cctcggagaa
    19741 ttggcagcgg ccgcggtcgg aagtggccgc gctcatgtcg ctgctgtcgc gcacgacgcg
    19801 gcgggaactc gatgaactgg tcaagcacga cgtcaagctg atcacggtcg ggcgggtgaa
    19861 cggtctttcc gccagccgcc agcgggtgct cgcccgggcg gtcgagaaaa cgcgccggaa
    19921 caaagggctc acgctctgtc tcgcgctcaa ctacggcggg aggaccgaga ttctcgacgc
    19981 cgtgaaagcg atcgccggcg ccgcacgggc ggggatgatc gacatgcctg agatcaccga
    20041 agaattgttt tcgagctttc tctacaccgc ggggctgccc gacccggacc tcctcatccg
    20101 gacttccggg gagatgcgga tcagcaattt tctgctgtgg cagacgagct acaccgagct
    20161 gtacgtgatc gacacgctct ggccggactt cggccgaatc gagttgttcc aggcggtggc
    20221 cgactaccag cggcgcgagc ggcggttcgg caagctcagc gagaaaacca ggttatgagc
    20281 cgcaacctgg ccttgcgaat tctcgtcgcc gccctcttca tcccggccat cctctggatc
    20341 ggctacgcgg gcgggtggtg gctctacgcc atgctgctgg tgtttctgac ggtcgggctc
    20401 ggcgagtatc tcatccgcga gggctatcgc ctcgaccgtc cggtattctg gctgacggaa
    20461 gccacagcgg tggcggttta ttcggtggtc gcgctccccc ggctgggggt cgagggtctg
    20521 ggtgaattcg tgatctaccg gtggcacgtc gcggcggccg gactcctggc ggctttcttc
    20581 ctcgtctcgg gcatgctgtt cacgctgggc aaacggccgc cggcggagtt gttcacgcgc
    20641 cactgccggc tgtgctgggg actggcgtac ctgagcatca cctggccgat cgtctttctc
    20701 gtcgggagcg gagtcgcggg tcagcacggc ggcgattcgc tgctgtttct gttcgggctt
    20761 ctgtgggtgg gggacacggc ggccatggcc gtgggcaaag gactggggcg gcacaaactc
    20821 tcgcccgcgg tgtcgccgaa caagaccgtg gaggggttca tcggggggat cgcgggagcg
    20881 gcggcgatcg gggtcgtgat gtatttctgg aaattcgaag ggcttggcct ggggcatgcg
    20941 ctcgcggcag ccgtgggcgc cagcgtgtgc gggcaactgg gcgacctggt cgaatcgatg
    21001 tggaaacggt cggtcggggt gaaggattcg tcggcgatca tccccggcca cggcggtgtg
    21061 ctcgaccgct tcgactcgct tctgttcgcg gccccgtttc tccttctgta tttcaccctc
    21121 atcagatgac ggaggggcgg agtgcggtgg ctcgattacc tatttgccgc ccggccgctt
    21181 ctccacgtgc cggtctggtc gatttacctg gtctcgctgc actaccacca gatggcgtcg
    21241 gggagagaat ttcacggcga agatctggcg actccggcgc tgttgagtct gctctgcgcc
    21301 ggcgcctact atctgaacca ggtgtatgat cgggaggggg atgcgctcaa cgggaagctg
    21361 ggatttctcg ggccgggacg cctgagcgaa cgcggcctgc tcggcgccca cctggcgctc
    21421 tcggtccttg cgctggcggg cgcggcgctc ctctcgtggg gcttgttgct ggtgttcgcc
    21481 cagctcctcg tgctcggcta catttattcg gcgccgccgc tgcggctgaa ggaccggccg
    21541 ttctgggggc tggcggccaa cgcctggagc ttcggcttgc tcatcccgct gaccgtgatg
    21601 cgggatctgt cggagcacac cgtggggctg ctgggcggcg acacgcccgt gtatttcctt
    21661 ctcgcagtgg ggggcacgta ccttctgacc accatcccgg accgcgccgg cgacggggcg
    21721 acggggaagc ggacgctggc ggtggtctgg ccggtggggt gggtgaaagc ggcggcgctg
    21781 gcgttgacgg ggctggcggc ggccgtggcg tggcgcggag ggttggccga actggccggg
    21841 gtcgccctta tctcgctggc gccgatagca gtaacgctcc tggccccaag cccatcgagc
    21901 gaacgcctgg cggccaaagc cccgatcgcg atcctgaccc tggcggccgg atggtggttt
    21961 cccgcatacc tcatctttgt cgttgtgctg gcggccgcca ccagcgtata ctaccgggcg
    22021 aggtttaacc agatttatcc acgattgacg tgatgcgagt taccgccatc ttgctgctgt
    22081 tgtacaccgg gctgctgttc ggctgcgccg gctacccgcg ctacaccggg ggactggtgg
    22141 agcggccggc cgaggtgggg gaacgccagg tggatttcac gaccgcggag tatgtccggc
    22201 tcgggctgat tgtgcagtcg ttcctcggca aaccctacgc cggcgcctcc ccctacgatc
    22261 ccggcatcga ctgctcgctc ttcattcgcg aggtgttcga aaagtacggc cggatcaaac
    22321 taccgcggac ggccgaggag cagtttgcgg cggggacaca ggtgcaccgg aatctgctgc
    22381 gctacggcga cctggtcttt ttccgcacgg tgccgggaca ggtgtcgcac gtcggggtgt
    22441 atctcggcaa cgacgacttc atccacgcct cgagctcgcg gggcgtgatc atcagcagcc
    22501 ttcgcgaaga atactgggcc cggtcgtacg ccggcgcccg gcgggttctc gactcgccga
    22561 aggggtgaac tcccgccggc cccgggcgtt ccacaaacat ggcaccgcac attgccgaac
    22621 ttgacctgaa actcaggaac ctgccgtcct cgccgggggt ctacctgttt cgcgaccgcc
    22681 gggggcgaat cctgtacatc gggaaagcca agattctgcg caaccgcgtg cggtcgtatt
    22741 ttcagcgggg aaacggccac gaggagcgga tcgcgcggat ggtggcgcag gtggccgatc
    22801 tcgaggtcat tgtcaccgac agcgaggtcg aggcgctcat tttcgaggcc aacctggtgc
    22861 gcgagcacaa accccactac aacgtcaacc tgaaagacga caaacatttt ccgtacatca
    22921 agatcacgac caacgaaccc ttcccgcgcg tcctgatcgt gcggcgcatc gagaaagacg
    22981 gggccaccta tttcggcccg tacacgagcg cgaaggggat gcggcgcaca gtggcgtttc
    23041 tcacgcagtt gttcaagatc cgctcctgca gttatgtgat tccgccgccc gagggaaaga
    23101 cgctgcgggt gtgtctggac taccatatca aacggtgcgg cggaccatgc gagggactgg
    23161 tggaccaggc gaactaccgg cgcggggttg aggcggtcat catggtgctg cgggggaaat
    23221 cgcgctccct gaccgagcag ctcaccgcgc gcatgcacga ggcctcggcc gcggagcggt
    23281 tcgaggaagc ggccgaggcg cgcgaccaga tcgaggcgat cgagtcggtc atgatcaagc
    23341 agaaggtcga tgtgggggag ccggtcgacc gggacatcgt ggccgtggcg cgcgagggag
    23401 gcgatgcggt cggcgtcgtc atgcagattc gcgagggggt gctgatcggg cggcaggatt
    23461 tccagttgac ggcgggggcg gatcgcgacg accaggcggt gctggcaacg ttcctcatgc
    23521 actactacaa ccaccagccc aaccttcccg acgaagtgca cctcccgttc gccatggagg
    23581 atctcgacct gttcgcggag tggctgcgga ggcagaaggg ggcgaaggtc aaagtgcagg
    23641 cgccgcagat cggggagaag tcgaagctgg tcgagctcac cgcgacaaac gcgcggctgc
    23701 tgctcgatga actcctgatc cagaagcggc aggtggcgga ccgcacgagc aggatggtga
    23761 ccggcctgaa agacgcgctg aaactcgccg tctcgccccg ggagatcgtc tgtttcgaca
    23821 tctcgaacac gggcgagacg gacgcggtcg ggtcggcggt gtacttctcc aacggcaagc
    23881 catacaagag cgagtaccgc catttcagga tcaagggggt gaaggggcag gatgatttca
    23941 agatgatgcg cgaggtggtg gggcggtatt ttcaccggcg cactgaagag cagaagcgca
    24001 tgccggacct ggttgtggtc gacggcggga aggggcaatt gtcggcggcg cggaaggagc
    24061 tgcagagagc ggggctggcg gcgcagccgg tgatcgggct ggccaagcgg ctcgaagaaa
    24121 tatacgtccc gggagcggcc gacccgatcg tgatcccgcg cagttctccg gccctgctgc
    24181 tgctcaaaca cgtgcgcgac gaggcgcatc ggttcgcggt cgagtacaat cggaatgtcc
    24241 ggacgaagcg gacgatcaag tcggtgctgg gggagatcag ggggatcggc ccggcccggc
    24301 agcaggcgct gctgagggaa tttgggtcgg tcgagcaaat ccggcagcag acagcggagc
    24361 ggctggcgga ggtgaagggg ataacgcggg cgctggcgga gcgggtgcta gaggcgctgc
    24421 gggagaaaag cggcgagggc ggggagtaaa atgcgccggc gggagccggg cggccccctc
    24481 tcctgcgggc ttgtgcgaaa gcaggaatgg accatctgcc cgacgggaat catcgcgctg
    24541 acgtccatgc ctcccgtccg gcacggatgg ctcgccgacg gcggccgccg ggggcgagga
    24601 tagtggttgc aatcggggac cggccgtgcc tatactcccc tcatgcagca gcccaaggcg
    24661 tacacggtca cggcgatcac ccggatgatc aagaatgtcc tcgaagaatc cttccagggg
    24721 atctgggtgg aaggcgagat ctccaactac atccaccaca cgtcgggcca ccggtatttc
    24781 agcctcaagg acgacaatgc cgtcctcaag ggggtgatgt ggcggtcgct ggggaacggg
    24841 ctgaaattcg agccgaaaaa cgggcagaaa gttctcgcgt tcggggatat cgcggtctat
    24901 gaaaaagggg ggcagtacca gctcaactgc aggaagattg tgccggtggg aatcgggccg
    24961 ctcgaactgg cctaccgcca gttgtacgag aaactctcgg ccgaggggct gtttgcggcc
    25021 gagcggaagc agcctctccc gcgctttgcc cagaagatcg ggatcgtgac ctcgccgacg
    25081 ggggcggcga ttcgggatat cgtgcagatt gcgcggcggc ggaaccggtc ggtcgagctg
    25141 atcctctatc cggcgcaggt gcagggggag ggggccgagg cgacgatcgc ggcgggcatt
    25201 cgctatttca acacgcgggc ggatatcgac gtgctcatcg tcgggcgcgg cggcggctcg
    25261 ctggaggacc tgtggccgtt caacacggag accacggtgc gggcgatcgc cgggtcgcgc
    25321 atcccggtga tctcggccgt cgggcacgag atcgatttca cgctggccga ctacgccgcc
    25381 gacatgcggg cgccgacccc ctcggcggcg gccgagctgg ccgtctggcc ggccgaggag
    25441 atgcggcggc tcgtgcggca gttggtggtg cggcaggcga cgctcctggg ggggcaggtg
    25501 cgggaggccc ggcggcggct tgcaggttcg ctctcgcgcc cggtgctcgc gcggccgatg
    25561 gagatggtgt tccaccgcca gcagcgcctc gaccacgccg cggaacggct ggcgacggcg
    25621 ggaaaaaaca gttttgaaaa gtacagaaac cgtctatctt tggtcgtgac ccggatggac
    25681 gctctgtcgc cgctggctat tctcgcccgc gggtattcgg tcacgcggcg gctacccggc
    25741 cagacgatag ttcgaagtgc gggggagacg gcgcccggcg accgcctgga gacgctcgtg
    25801 gtcgacggcg agatcatttc ggtcgtgcag gaatgcaggc ggaaacagga tgacgaacaa
    25861 gaagaagcct aaagatttcg agagcgccct ggaacggctc gaggcgatca ccgagcagtt
    25921 ggaggcggga gaatcgtcgc tcgaagagtc gatccgcctg tacaccgagg ggttggagat
    25981 cgcccggttc tgcgaccgca aactcggcga ggcggaaaaa cggataaaga tgatccagga
    26041 atccgacggg gcggcggtcg aggtggaaac ggaagccgac gcggccgacg acgacgggga
    26101 ggaggacgac gaggacgagg aggggggaga cgggacggga tggccatcgg gacggtgagt
    26161 atcggcgcct atctccgcga gcagagcgcg gtggtcgagg cccttctcga ccagtacctt
    26221 ccgccggcga gcgccccgcc gcagcggctg cacgaagcga tgcggtactc ggtgctggcg
    26281 cccggcaaac ggctccggcc gattctcgcc cggctcagtt tcgaatactg cggaggggac
    26341 gcctccggga ccgataaacc gattcactgc gccatggcgg cgctcgaaat ggtgcacacc
    26401 tactcgctca ttcacgacga tctgccgtgc atggacgacg acgtcctgcg gcgggggatg
    26461 ccgacctgcc acgtcaaatt cggcgaggcc acggcggtcc tggccgggga cgcgctgcac
    26521 gacgtggcgt tcgagctgct ggcgcggacg ggggaaatcc aaacggtggc ggagctggcc
    26581 gaggcgctcg ggacggccgg gatgatcggc gggcagatgg cggatgtgga ggcggaggga
    26641 cggccggtcg gccgggggga cgtcatgagg atccacaccc gcaagaccgg ggcgctcatc
    26701 cggtgcgcgg cccggctggg aggaattctc gccggagccg gcgagccgac gatggcgcgg
    26761 ctcacgcgct acggcgaaaa cgtggggctg gctttccaga ttatcgacga catcctcgac
    26821 gtcgaggggg accgggaatt gctgggaaag aacgtcggat ccgacagtaa aaagcttaag
    26881 gcgacctacc ccggcgcggt cggtctggag gcggcgcacc gggacgccgc ggcgctcgtg
    26941 cacgaggcgg cggccctgtt tgccgccgaa agcgacagcc ggctgagcga actggccctc
    27001 tatatcggtc aacggaaccg gtgagcgggg acggagaaga agcatatgaa gcatctgcag
    27061 aatatcaact gcccggcgga tctcaagcgt ctgtc
//
LOCUS       DAOPOE010000041        26873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2305_length_26873_cov_5.498658, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000041 DAOPOE010000000
VERSION     DAOPOE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26873
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_26873_cov_5.498658"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..826)
                     /locus_tag="PKM94_07160"
     CDS             complement(320..826)
                     /locus_tag="PKM94_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD66457.1"
                     /translation="MYHSLKESLESWKGESERTQQVLDALTDASLAQSIAAGHRTLGR
                     IAWHIVTTIPEMMTYTGLKLENLLNPDKPVPAAAAEIAEAYRALSAALVDQVSGTWND
                     RSLDIEDDLYGQKWPRRITLMVLIQHQIHHRGQMTVLMRQAGLKVPSIYGPSLEAWQE
                     HGMNAPEV"
     gene            complement(986..1342)
                     /locus_tag="PKM94_07165"
     CDS             complement(986..1342)
                     /locus_tag="PKM94_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOD66458.1"
                     /translation="MFRPVILSCRAAAVLSLALAGGGCGEPGTPGATAAADSAVVLVV
                     ADDSATVLELTRRQHRVECRNTAMGAFVTGIDTLRAGGQAYWVYSVNGTTPDRAADKF
                     LAAPGDTIRWRLRKVQ"
     gene            1469..2356
                     /locus_tag="PKM94_07170"
     CDS             1469..2356
                     /locus_tag="PKM94_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66459.1"
                     /translation="MNYSEIISSSFRLAWQNKSLWLLGLFSSGYGLYSFQSTFDLDSE
                     HPWIDLSGLGGYDFSLGGWLLAAALLVVLLMAATAVFLTALCTPAIIDAVNRLSRGGR
                     YSLGASFAAGFRFLGRALGVLILHFLVYIAFFAITVPLGALALLVHPLLLVAAVLTGI
                     PLLMLFVFAAETVFQLAYRAMVVRDIPIAAALREGLGLLGRYPGPCAILFLLHVGLYI
                     VTAMIAAGAIVVMAIPFVLLAMSSSAGLIAALAIGIPLFWLVSLPAGGFLGAVFETMY
                     TLFYFRLLEPIPAGAPATA"
     gene            2430..3530
                     /locus_tag="PKM94_07175"
     CDS             2430..3530
                     /locus_tag="PKM94_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD66460.1"
                     /translation="MKALFLIMLVAAVLPAQAQEQAVPASPPAGPIPQVRLNYGAVEL
                     QRALYPVHYGASSARADLRWAQEHNAGLESFWAAQGDSILSLLAALSGIAWTESAFDL
                     FVLRYFLSTGAPEPLIIPTGGIRRGEAIEAAPAGKVMKLNVIYQLARRLLDQASESAG
                     HSGLADHPLLRPGPYRRDILALVLAGAAAEGVLGRDSAQGAIRSAFWRNHFPGIEIYE
                     SYFAGGDWALSPQDPLVTRVLAEPPTSALMAATNPAPRPESTGKRTSVEGLPLSGELG
                     MAISVADNGLLRVDALDPQRAAYASGLREGDLIRTVDGRRVKNHRELVEQILKTMTRR
                     GATLQLVREGGAKAVVVRPVTAAERPGNGDAD"
     gene            complement(3628..5619)
                     /locus_tag="PKM94_07180"
     CDS             complement(3628..5619)
                     /locus_tag="PKM94_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOD66461.1"
                     /translation="MNARCSRRYRLSLLLLVTLLLCLPAAVSAQDDNETCLSCHSDNE
                     LTGLNRKGAEISMFVAPGSLDSSVHAGMSCIDCHTDLAGNADYPHAEELQPVDCGTCH
                     SDVAELYATSAHATSNPNAPTCASCHSHHNILSDTNPEAPTSAGKLPYTCSSCHSKQT
                     LTRDPDVRIADSFDRYMRGIHARGIQKGIGSAASCNDCHGMHSLKRASDPQSLVHKMN
                     IPKTCAKCHNDIYIQYARGIHGKALAQGILDSPNCSDCHGEHEILEVDREGSPVNHAN
                     LADYVCGKCHTDPQMVEKYGLSNNQFTSYQDSYHGLAVRGGSLKAATCVSCHEAHAIL
                     PQANPASSIHPDNVTATCQQCHPKANAKFAQSYTHQAFIAGDNRIDDVVETIYVWLIV
                     ITIGAMLVHNLIIVGRYVVEKHRKMKGQPTVQRFTGAMIYQHMVVTIAFITLVLTGFA
                     LRYPDAWWVHILNFLHLNEGSRSVIHRIAAVLLMYIAVHHGLYLFLARKGRKELRAIW
                     LSTQDLKHLVQNVRYHLGMTREKPRFGRFDYTEKAEYWALVWGTVVMALTGFILWFPT
                     FFTGFLPAWIVKVAETIHLYEAWLATLAIVVFHFFFVIFHPEQYPMSFTWLTGRMTVK
                     ECEEHHPEWYECIASGEHREHVGAGPDPGRSGDRPAKQD"
     gene            complement(5705..8614)
                     /locus_tag="PKM94_07185"
     CDS             complement(5705..8614)
                     /locus_tag="PKM94_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD66462.1"
                     /translation="MRRLLPLVLLALLPALPALAQDNETCLSCHADEDLAGTLHGREI
                     SMHVGEDALAGSVHAELECIMCHTDLAGVEDWPHAEDLAPAACGDCHDAGALRTSAHG
                     TAAQCWDCHGSHRILSPADSDPETGGVRCGVCHETTAKIYYQSLHGRLVHEGAELAPR
                     CWDCHGGHNILPRTDPNSQVAKFNIPFVCGECHKEGSPVHQRYDIPQDSIMQHYSESI
                     HGIGLFKQGLTVTAVCSDCHTAHNVRDHADPQSSIHRNNIAATCRQCHGRIEQVHQQV
                     IRGELWEKEPGKVPACVDCHSPHQIRRVFYQEGMSDRECLACHGRPNLTMVRGGKTVS
                     ISVDSLETHSSVHRGVTCAQCHTGVTPRHNERPCATVVNRVDCSICHAEVVATYREST
                     HGQLVDRGDPDAPECIDCHGVHGVLGRKDPQARTFPTNVPRLCAECHRAGARAAARIN
                     EGHDRVEEYIESIHGKGLLQSGLVVTAMCTDCHTAHHVLPQTDARSSVHRANIPKTCA
                     RCHNGIYEQYAKSIHSATANPDAKDPLPICNDCHHSHTITRADLADFRLTVRKQCGAC
                     HADVTESYFETVHGKVSQLGGTAAAQCYDCHGAHDTLPPSDPGSHLSRAHIVETCGQC
                     HKGSHRQFAGYLTHATHHDRTKYPILFYAFWFMTTLLVGTLIVAGAHTILWLPKSWQM
                     MREHKQVRTQYRGSLEYRRFKPLHSRLHIMVVTSFIALAITGMTLKFSYLGWARWISE
                     LLGGFESTSYIHRVAAVVTFAYFGIHLWDLISEKRRQRKSWREYLFTKNSMLPNRDDL
                     REAIATIKWFLGRGPRPRYGRWTYWEKFDYFAVFWGVAIIGTTGLMLWFPEFFTRFLP
                     GWIINVAQIVHSDEALLAVAFIFTVHFFNTHFRPDKFPMDTVIFTGRVPVEELKNDRP
                     REYEELIQSRKIKDHLVPPLPPVMVRTAKVFGAVALTIGLILILLIIYAEIFGYR"
     gene            complement(8640..10127)
                     /locus_tag="PKM94_07190"
     CDS             complement(8640..10127)
                     /locus_tag="PKM94_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOD66463.1"
                     /translation="MRHPLAALGGALVLAGGALFIILVLIDLSAAHENMYRLLVTFIL
                     SPAVILLGVLLFLISVRLQIGRARRAGEDVRFTLSIDPADPKYIRNLWLFLAFTAVLV
                     LVVVYAGTEAYDATESVVFCGETCHRVMEPQYVTYHNSPHARVACAECHIGPGASFWV
                     KSKVDGLRQVWATALNTYPEPIPTPIVNLRPARETCEQCHWPEQFYGDKLLVRTYYRT
                     DEQNSPWTVALRVKIGGGTPRATGQAGGIHYHMLTERRLEYIPADEKRSVIAWVRSIG
                     PEGDTTVWVNADAEQPAVADSADAIRAFDCMDCHNRPSHRFNPPATAVNRALNRHELS
                     AELPSIRKLAVDLLNAEYKTKPQALEAIAQGLREYYRTNYPDRVEAQKDDIERAAAVL
                     QEIYTLNFFPEMKTDYRVRENHLSHFVSDGCFRCHTPSMTNAAGEPMKNDCRTCHLIV
                     AQGPSTAIDSLEMNLAGLEFRHPEDIDEAWREVNCTACHTADQGY"
     gene            complement(10363..10815)
                     /locus_tag="PKM94_07195"
     CDS             complement(10363..10815)
                     /locus_tag="PKM94_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66464.1"
                     /translation="MRVSEESADSVIAPFHYGTTYEMAGYTTNAHTFYLSAAYQPIAK
                     MRLTSTLTYNLSKAGFEEGIMPDGRAFYDGDRVLEHMDFSFAEMDAYSDLDFEILRFA
                     AGVEYQFTPTFTFTLDGDVAHVVDYQGWVWGDETGRYYMVRSGVLVNF"
     gene            complement(10849..10974)
                     /locus_tag="PKM94_07200"
     CDS             complement(10849..10974)
                     /locus_tag="PKM94_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66465.1"
                     /translation="MSRFAIAPNLNVIRTPDDRFSATIGYTFNHYRSTGPVSVAL"
     gene            complement(10986..11486)
                     /locus_tag="PKM94_07205"
     CDS             complement(10986..11486)
                     /locus_tag="PKM94_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66466.1"
                     /translation="MRPSAITSVLCLALLAVAAPRAAQEEAESYDEYSTWLGGHYSEF
                     LNDGKKAGEYRIIEDDGWAEVRLRLLSRRGNSLLTANGHFFDKDNLQARMSLVNGRTL
                     DLDFGCRTLIHQLGQDRLLDFAAREWAAGADTLGGKMLTHTILDSLVDCHVSRREMLT
                     RIDLQL"
     gene            complement(11497..12438)
                     /locus_tag="PKM94_07210"
     CDS             complement(11497..12438)
                     /locus_tag="PKM94_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HOD66467.1"
                     /translation="MGCLISGGLRIPRAALAAAGLALALLLVGPAVAEVPDSACAGCH
                     DIVDMFHKSPHGAYFAGDPALAAGTCEACHGSAVDHVASGDPADIINPARADQFGAAA
                     LCLNCHKNHQFDDWAFSAHYAAGVTCASCHRIHAEYTDDRLRDPALCYSCHAEVRAAA
                     YMPSHHPIAEGKMRCLDCHSVHGGGTMFVKDMTNGELCFSCHAEKEGPFIYEHSPVQE
                     NCMICHSPHGTVANSLLKQNEPALCLDCHPMHFHAAVPGIGGTFQPPAAPERRTVSTR
                     ESRRIGMLTKCTQCHTQIHGSDLPSQSMSARGAGLTR"
     gene            complement(12426..12866)
                     /locus_tag="PKM94_07215"
     CDS             complement(12426..12866)
                     /locus_tag="PKM94_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66468.1"
                     /translation="MAALYGERRRVAKRFAPGERRRGCPSKITAAPGVFVKKVTTRWA
                     VRYFFTWPVRSPRLVITDKFAGIGDSAVTDRKADIPDEAVNLFVRLGGGGKTTKPLAC
                     GISTPPLRRRPQERPQTGRETRKAKINCQGVSQHEQTEEVSWVV"
     gene            12915..13448
                     /locus_tag="PKM94_07220"
     CDS             12915..13448
                     /locus_tag="PKM94_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66469.1"
                     /translation="MHDLVARLLPEVNEITDPTLREKTIACWADAIAFRGWTEHLLRN
                     IPFTLLAQNVKITFVDHVRAVCRMCLACDDVLTEVHGANRTRVNRDYLIAGALLADVG
                     KLMEYEIVDGKPVKSDYGKHLRHPFSGVGLAFRHGLPSEVMHVIATHSKEGEGEKRLP
                     ESIIFHHCDFIDFDLVK"
     gene            13522..15495
                     /gene="lysF"
                     /locus_tag="PKM94_07225"
     CDS             13522..15495
                     /gene="lysF"
                     /locus_tag="PKM94_07225"
                     /EC_number="4.2.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase"
                     /protein_id="HOD66470.1"
                     /translation="MSHSQTLVEKIAQRYAVGLEPGHAVRAGDYLSIRPAHVMTHDNT
                     GAVIPKFQSIGAARVANPRQPVFTLDHNVQDTTESNLAKYRKIESFAAAHGIDFYPAG
                     RGIGHQIMCEEGYAWPGTMVVASDSHSNMYGGLGCLGTPIVRTDAAAIWATGRTWWQV
                     PPVARCQLKGQLPPGATGKDVIITLCGYFNKDEVLNHAVEFAGDGLRSLSVDERLAIA
                     NMTTEWGALVGLFPIDDVTLDWLRRRIEFVRRRGLAGVPSDADGGGEHPRLRTARLED
                     LQRHIPAADPGAFYARELMLDLATVTPHVSGPNHVKVMTSVADLARQKKRIHKAYLVS
                     CVNSRVEDLAAAAAVVRGKRVAAGVEFYIAAASSEVQAESEKRGDWAALLAAGAIPLP
                     AGCGPCIGLGRGLLGEGEVGISATNRNFKGRMGAREAEAYLASPAVVAASAIAGYITG
                     PAEATARPTTGEMRVNRKPAGVTKAARVIDGFPTRLSGRLAFCRQDNLNTDGIYPGKY
                     TYIDDMTPEQMAAVVMENYDPEFGKLVRKGDILVGGFNFGTGSSREQAATALQHRGIA
                     LVVAGSFSETYKRNALNNGFLAIECPDLVNDLKAACDPNRLTAVSDSEATVDFAAASL
                     VFAGKTYEVDPIGAAAQELVLSGGLEGWVRQRL"
     gene            15548..16309
                     /locus_tag="PKM94_07230"
     CDS             15548..16309
                     /locus_tag="PKM94_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66471.1"
                     /translation="MALKYHSSVASALAALIAAGPLFLACGSDDGNPFGGGGPDHALN
                     PAEYFGLVPGRTITAGYVNYVGDSLRQFVPNSPVDAWSFTPVIGLPTICNGRLAYPVS
                     AAPIPSNYSDPDYAFTLYFSADSSGLYWHGGSYCGLDLPLPSPGRIAPYPLASGLKWQ
                     TIGHAETDIDQCNRSVADTLNATCRRITNAVVRGNAYPEVFLIRIPFIDVPGHIGTRY
                     YEAWIAPGYGPVRGASFDQAYEHPDEGFEFISYSP"
     gene            16387..17598
                     /locus_tag="PKM94_07235"
     CDS             16387..17598
                     /locus_tag="PKM94_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOD66472.1"
                     /translation="MDWLIILVFGVVYLGMILGRIPGLALDRTGVALLGALCLLGFGR
                     VTTADLPAAVDMSTIALLFALMVVSSQFRLAGAYSFLVRRAGELAVSPAALLAVVIGV
                     TALLSAVLVNDIVCLAATPVVIEACAHRRLNPVPYLLGLAAAANIGSAATLIGNPQNI
                     LIGEVLRLSFSGYLLESLIPVVFGLVLLWLILAWQYRGRLGAETVAPPVAAPLFSRWQ
                     TAKGTLVLAALLVIFLAGSWPRDLAALGAAGVLLLSRRMQSREMLGLIDWPLLVLFLA
                     LFVVNYAVNSGGYMAGVMVGLDAAGIPLDHPGWLFAASVVLSNLVSNVPATMLLLPSA
                     THPLGGAVLALSSTLAGNLLVVGSIANIIVLDQAERLGVRISWRRHARTGVPVTAATL
                     LAAGVWLWLRA"
     gene            complement(17603..19048)
                     /gene="amrB"
                     /locus_tag="PKM94_07240"
     CDS             complement(17603..19048)
                     /gene="amrB"
                     /locus_tag="PKM94_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD66473.1"
                     /translation="METDRATIRKPAVAGAFYPRDPGELATAIARLYAQAGKRIITGH
                     PVVVVAPHAGYPYSGLTAAKAYKQLEGEQFDTVVVISPSHTVFFKGSSVFAGAGYETP
                     LGVVETDRPLADRIAAINPSNVYYSNMGHATGSTRGEHALEVQLPFLQVMLGRFKLVA
                     IVMGDQEEESVRILGETLAAALKGANALMVASSDLSHFHNAKVASRLDGTVRQAVEQF
                     DEAFLLETLETGKGEACGGGPIAAAIIAGRRLGATRAQVIDYSHSGQTTGDFNEVVGY
                     LSAVFLSERKAAAREVIGARAAGEPRKREERIADDDRRLLAAVARDAIRARLEGREYA
                     PPAPESLAMQKGAFVTLHLQGQLRGCIGRLRSDEPLPRTIADMAVAAAFEDPRFPELH
                     ERELDRIDIEISILSPLARVRDIEEIQVGRDGLMIKLDWHSGLLLPQVAAENGWDRME
                     FLGQTCLKAGLPKSSWKDKNAEIYRFSAEVF"
     gene            complement(19119..20093)
                     /locus_tag="PKM94_07245"
     CDS             complement(19119..20093)
                     /locus_tag="PKM94_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD66474.1"
                     /translation="MDTGILIFLLAAIAFAVVIVSFSVKVVGPYERGVVVRLGKYQRV
                     LAPGLNVIFPLLDDVRKIDMREQVLDVPPQQVITKDNVVVEVDAVIWAQVTDPVRATF
                     EISNYFDAASKLAQTNLRNVMGEMDLDTCLSSRDKINGQLRDVLDDATDKWGVRINRV
                     ELQRIDPPRDITEAMSRQMKAERDKRAAILEAEARRQAAILLAEGNGKAAVLEAEGFA
                     EAVRMKADAERYRQITVAEGEALAINNVFGAIHASRPDDKLIMLKYLEMLPKLAENQA
                     NKIFLPYEASGMIGALAALIEGARPNGGGKTASPRPAIAPVDTEEVAG"
     gene            complement(20099..20539)
                     /locus_tag="PKM94_07250"
     CDS             complement(20099..20539)
                     /locus_tag="PKM94_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOD66475.1"
                     /translation="MATMFWIWMAAAVIFLIIEITSPSFLAICFAIAAAAAGVFAQFN
                     PESYYWQVGLFIIASLVLLPLMRPLARRITKPSPVQSNIDRLIGQIGIVTKPIDRESP
                     GQIVVDGETWRAVAAGTFAPAERVRVASVQGNRLRVERAEESEE"
     gene            20938..21288
                     /locus_tag="PKM94_07255"
     CDS             20938..21288
                     /locus_tag="PKM94_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD66476.1"
                     /translation="MTNVARRETNGMRNRLPSPFFEDFFHWPPAPWEPHHWPRVDVRE
                     TSEDATLLFEAPGMRKEDLEVQVGNNRLTVSGERRLEADDSDEGWGPPRDRDRDIQAP
                     VYAPVGYRPALGQG"
     gene            complement(21439..22644)
                     /locus_tag="PKM94_07260"
     CDS             complement(21439..22644)
                     /locus_tag="PKM94_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66477.1"
                     /translation="MSVPRWSYRKSLASLLACLILLQAAAVIVAGPDRPPWGDESHFV
                     DTVRLFASRPSLETLRTYPEMSGPLPFALYAAWGHLAGLGLWNLRLLSLVIALGSYLV
                     FHRLLWRMCGDGRLAFLFALLFALQPYMIGFSIFVFTDGAAILFLLAALWGAVAGRAV
                     LILLGLAGALLCRQYAVFAVMAVAAWALLLRNSPERREFAGRALGAAAAAAVPLLTLF
                     LLWGHVSPDNRLRGLYLGGGLAFHPSALVLYISLLFWYLLPAAAYAGRRVYTRGRLIA
                     AAALSPLYWLFPVEASPAAKAIGVQTVGLLHKAVENFPAAPWLPHAFFFAGFLFGLPI
                     VLHIAQSAVARIRARTGDVVLLLDSGVIAFLLVMPFSYLAWEKYMMPLAPLVLARLAA
                     LRVPGDPAS"
     gene            complement(22804..24216)
                     /locus_tag="PKM94_07265"
     CDS             complement(22804..24216)
                     /locus_tag="PKM94_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66478.1"
                     /translation="MSLPRSAGRGILGPGALLLVLAAGCSSGLNREKFYAPLEADLRS
                     GNYAAAVATVEEARSKGAYKEKDRFLYYLDAGLAYHYASQFDSSNLRLAVAERAAEEL
                     FTKSISKGVLANTLLNDNVLEYPGEDYEVLYTNLLMALNYLALGKHDDAFVEIRRAHD
                     KLNLLEQKYADAAAAIAADAARDTTGACVAFAGTDVRFNSSAFARYLSMHMYAAEGLD
                     DDARIDSQALADAFARQPNIYAFPPPPVAYAADSGGVLSVVGLVGLAPVKQPLNLRVR
                     TDENLHLLTVIYDDARPEHTVFANYPLPEGIGDFYAKLSLPQLVPRPSEIAAVRVSSG
                     GRVLGELQLLEDVAAVAAASFEARQSIILWRTVVRVVLKTLSASRLKNAVDNGGIAGW
                     LGKLAADVAYDVSEQADLRSAVLLPGRIFVGDFVVPPGAYDLTVEYLDAEGAVVESRH
                     YPGVSVRERAFNLVRTFCLR"
     gene            complement(24213..24815)
                     /locus_tag="PKM94_07270"
     CDS             complement(24213..24815)
                     /locus_tag="PKM94_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOD66479.1"
                     /translation="MTMRYLLVVLALAALAIGCGGGQTVTRLDPGGTTDLSGKWNETD
                     ARLVAEEMIADALARPWLMNFVEKNGEKPVVTVGTIRNKTNEHIDTEVFTADFERELI
                     NSGNVRFVGSHDERYEVREERQDQAEFASEETRKLLHDELGADFILLGAIKTITDAVE
                     GRSTVFYQTDLELINIETNEKVWIGTKKIKKGIAQSGTKW"
     gene            complement(24991..25680)
                     /locus_tag="PKM94_07275"
     CDS             complement(24991..25680)
                     /locus_tag="PKM94_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOD66480.1"
                     /translation="MDSFAVAAATAFWLGLLTSVSPCPLATNIAAMSYVGRRIERPRL
                     VLLSGVLYTVGRLAAYAALAALIVSSLTAVPELARWLQRWMNVVLGPILILAGLFLID
                     LIPLRLPGIGVGRRLEQRVDRLGIWGGGLLGVMFALSFCPVSAALFFGSLIPLALEQR
                     SGFLMPVLYGLGTALPVVVFAALIAFGARFIGSVFHRVNAFSRWARRLTGVVFIAVGA
                     YYAVLYIFGSR"
     gene            complement(25680..26165)
                     /locus_tag="PKM94_07280"
     CDS             complement(25680..26165)
                     /locus_tag="PKM94_07280"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOD66481.1"
                     /translation="MNPVAFTMLLFGCLLGAALPASAAEEPAAPAADTAAIDSVEPAP
                     AEAPVQVIATYFHGNRRCATCRKLEAYAQEAVTGGFPDEIAAGVLQWRVVNFDEDANA
                     HFLEDYELFSQAVILSRLEGGKETVWKNLDRIWTLVGDQEAYLAYVRSELRTFLAAGA
                     D"
     gene            complement(26196..26429)
                     /locus_tag="PKM94_07285"
     CDS             complement(26196..26429)
                     /locus_tag="PKM94_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOD66482.1"
                     /translation="MKKIQVFGPGCAKCEELARLAAAAVAELGLACEVEKVSDIRAIM
                     EAGVMITPALAVDGRVLVAGKVPSLAKLKEILA"
     gene            complement(26448..>26873)
                     /locus_tag="PKM94_07290"
     CDS             complement(26448..>26873)
                     /locus_tag="PKM94_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOD66483.1"
                     /translation="GEPGEWFAQTWGFAKQILPLLVIGVLAAGALLGRPGEEGLIPQT
                     WVAAAVGGNSLQANLLASVIGAFMYFATLTEIPILQGLIGSGMGPGPALALLLSGPAV
                     SLPSMLVIRSVIGTKKTVVYVLLVVGMATLSGLIYGALL"
BASE COUNT         4491 a         8224 c         9502 g         4656 t
ORIGIN      
        1 gggcttcggc tattaccaca aacccccgcc accgccaccg cgccggcgcg cctgttcggc
       61 aaaacccagg cgacaggaga gcgcccacag cctctcccct gactcgggtt cttgagcgat
      121 gcggattgaa cctcccgccg gagaaccagg cggaggggga attacaaatc acagatcaca
      181 tgtggcgagc gctctgtcgc gttggcgggg ggacggacgg cggcggcgat tcagagtgac
      241 ggttcgcccg ccgccaccga ctggcggtga cggcaggctt tagtcattcg ggagcccgac
      301 agacgggccg aggcggcgat cagacctccg gggcattcat gccgtgttcc tgccaggcct
      361 cgagcgacgg cccataaatc gaaggcactt tcagtcccgc ctggcgcatc aggacggtca
      421 tctgcccccg gtggtgaatc tggtgctgga tcagcaccat cagggtgatc cgccgcggcc
      481 atttctggcc atagagatcg tcttcaatat cgagcgaccg gtcattccac gtaccgctga
      541 cctggtccac aagggcggcg gaaagggccc gataggcttc ggcgatctcc gccgcggctg
      601 cgggaaccgg tttatcgggg ttgaggagat tctcgagttt caggccggtg taggtcatca
      661 tctcgggaat ggtggtcacg atgtgccagg cgatccggcc aagcgtccgg tggccggccg
      721 caatggactg ggccagggag gcatcggtga gagcatcaag aacctgctgc gtcctttcgg
      781 actccccttt ccaggactca agagattctt tcagggagtg gtacacggga gactcctttc
      841 cggtctatgg atcagttcgc ttcatgtcag ccgcgcttga gttgcacgcg actggggtcc
      901 tgcggcaggc gcccgggcac tcccgccgcg tcggcggcgg cgcgaccgcc cggagcgcac
      961 tgcggtcagc cggcgatcgc accggtcatt gcactttccg gagacgccag cggatggtgt
     1021 cgccgggagc ggccaggaat ttgtcggcgg cgcggtcggg tgtcgtgcca ttgacggagt
     1081 agacccagta ggcctggccg ccggcccgca gggtgtcaat gccggttacg aacgcaccca
     1141 tcgcggtgtt gcggcattcg acccgatgtt gccggcgggt gagctccagc acggtggcgg
     1201 agtcatcggc aaccacaagc accacggcgc tgtcggccgc ggcggtcgcg cccggagtgc
     1261 cgggctcccc gcaacccccg ccggccaagg ccagcgacag gacagcggcc gcccggcacg
     1321 acaatatgac cggcctgaac atacggtgaa gtaaccatgc ttttcccgac cggacaagtc
     1381 ctttggcgag gcaattccgg ttgtccttcg cagatcgggt gaatactgcg cggtttccgt
     1441 gagtaatatc catgtcagga gaatggctat gaactacagc gagatcatca gcagttcctt
     1501 ccgtctcgct tggcagaaca aatcgctctg gctactgggg ctgttttcat cgggctacgg
     1561 actgtactcg tttcagtcga cttttgatct ggactcggag cacccgtgga tcgacctgtc
     1621 ggggctgggc gggtacgact tctcactcgg cgggtggctt ttagccgccg ctcttctggt
     1681 cgttctgctg atggcggcga cggcggtgtt tttgacggct ctgtgcacac ccgcgattat
     1741 cgacgccgtc aaccggttat cacgcggcgg gaggtactcg ctgggcgcgt cgttcgcagc
     1801 cgggtttcgt ttcctcgggc gggcgctcgg cgtgctgatc ctgcactttc tggtctacat
     1861 cgctttcttc gccatcacgg tgccgctcgg cgcgctcgcc cttctcgtac acccgctgct
     1921 gttggtcgcg gctgtgctga ccgggatccc gctcctgatg ttgtttgtct ttgcggcgga
     1981 gacggtgttc cagctggcct accgcgcgat ggtggtgcgg gacattccga tcgcggcggc
     2041 gctccgcgag ggtctcggtt tgctggggcg gtatcccggc ccgtgtgcaa tcctctttct
     2101 gctccatgta gggctgtata ttgtcaccgc gatgattgcg gcgggggcga tcgtcgtgat
     2161 ggcgatcccg tttgtgctct tggcgatgtc ctcatcggcc ggcctgatcg cggcgctcgc
     2221 gatcgggatt cccctgttct ggttggtgtc gctgccggcg ggcgggtttc tcggggcggt
     2281 gttcgagacg atgtatacgc tgttttactt tcgcctgctc gagccgatcc cggcgggagc
     2341 gccggcgacg gcgtagcggg agccgggggg ctcggtcgga ccgagagttt catcttgcga
     2401 tcgccggtcg gtgcggttta tttggcgtca tgaaggcgct tttcctcatt atgttggtgg
     2461 cggccgtgct gccggcccag gcccaggagc aagcggtccc ggcgtcaccg ccggcgggcc
     2521 cgattccgca ggtacgtctg aactacggcg ccgtcgagct ccagcgggcg ctgtacccgg
     2581 tccactacgg cgccagcagc gcgcgcgccg acctgcgctg ggcgcaggaa cacaatgccg
     2641 ggttggagag tttctgggcg gcccaggggg attcgatcct ctccctgctg gcggccttgt
     2701 ccggaattgc ctggacggag tcggcattcg atctgttcgt gctgcggtac ttcctctcga
     2761 cgggcgcgcc ggagccgctc atcatcccga ccggggggat acggcgcggg gaggcgatcg
     2821 aggcggcacc ggccgggaag gtcatgaagc tgaacgtcat ctaccagctc gcgcggcggc
     2881 tgctcgacca ggcgagcgag tccgccggcc attcggggct ggcggaccac ccgctcctgc
     2941 gtccggggcc gtaccgccgg gatatcctcg cgctggtgct cgccggggcg gcggcggagg
     3001 gtgtgctcgg gcgcgactcg gcccaggggg ctatccgctc ggcgttttgg cggaatcatt
     3061 ttccggggat cgagatttac gagagctatt tcgcgggcgg cgactgggcg ctgtccccgc
     3121 aggacccgct cgtcacccgg gtgctggccg aaccgccgac ctcggcgctg atggcggcga
     3181 ccaacccggc gccgcggccg gagagcacgg ggaaacgaac ttccgtcgag ggactgccgc
     3241 tgagcggcga gctggggatg gcgatcagcg tcgccgacaa cgggctgctg cgagtggatg
     3301 cgctcgatcc ccagcgggcc gcgtatgcgt cggggctgcg ggagggggat ctcatccgga
     3361 ccgtggacgg ccggcgggtc aagaatcacc gggaactggt ggagcagatt ctcaagacga
     3421 tgacgaggcg gggggcgacg ctgcaactgg tgcgggaagg cggcgcgaaa gcggtcgtgg
     3481 tgcggccggt gacggcggcg gagcggccgg ggaacgggga cgccgactag aaagcccgcc
     3541 ggtgcgcggc gcagaatcaa aaaccgggcg cccgacggcg cccggtccta gctccctcta
     3601 ccgacaatcg tatccgcgca cgggtcctca atcttgtttg gccgggcggt cgccggagcg
     3661 gcccgggtcg ggacccgccc cgacgtgctc gcggtgctcc ccggaggcga tgcactcgta
     3721 ccactccggg tgatgctcct cgcactcctt gaccgtcatc ctgcccgtca gccaggtaaa
     3781 cgacatcggg tactgctccg ggtggaagat cacgaagaag aagtggaaca cgacgatggc
     3841 gagggtggcg agccaagcct cgtagaggtg gatggtctcg gcgactttca cgatccaggc
     3901 ggggaggaag ccggtgaaga aagtggggaa ccagaggatg aagccggtga gggccatgac
     3961 gacggtgccc cagacgagcg cccagtactc ggccttctcg gtgtagtcga aacgtccgaa
     4021 gcggggtttc tccctggtca tgccgaggtg gtacctgacg ttctggacca ggtgtttgag
     4081 atcctgggtg ctgagccaga tggcacggag ttctttccgg cctttgcgcg ccaggaagag
     4141 atagaggccg tggtggacgg cgatgtacat gaggaggaca gcggcgatgc ggtggatcac
     4201 cgagcgcgac ccctcgttga ggtgcaggaa attgaggatg tgaacccacc aggcgtccgg
     4261 gtagcgcagg gcgaagccgg tcaggacgag ggtgatgaag gcgatggtga caaccatgtg
     4321 ctggtagatc atggccccag tgaaacgctg gacggtgggc tggcctttca tcttgcggtg
     4381 tttctcgacg acgtagcgcc cgacgatgat gaggttgtgg acgagcatgg cgccgatagt
     4441 gatgacgatc agccagacgt agatggtctc cacgacgtcg tcgatccggt tgtcgccggc
     4501 gatgaaggcc tggtgcgtgt agctttgggc gaacttggcg ttggccttgg ggtggcactg
     4561 ctggcaggtg gccgtgacgt tgtcggggtg gatggaggag gccgggttgg cctgcgggag
     4621 gatggcgtgg gcttcgtggc acgacacgca ggtggcggct ttgagcgacc cgccgcgcac
     4681 cgccaggcca tggtaggagt cctggtagga cgtgaactgg ttgttgctca agccgtattt
     4741 ctcgaccatc tgcgggtcgg tgtggcactt cccgcagaca taatcggcca ggttggcgtg
     4801 gttgaccggc gagccctccc ggtcgacctc gagaatttcg tgttcgccgt gacagtcgga
     4861 gcagttgggg gagtcgagga tgccctgggc gagggccttg ccgtggatgc cgcgggcgta
     4921 ctggatgtag atgtcgttgt ggcacttggc gcaggttttc gggatgttca tcttgtgcac
     4981 cagcgactgc gggtccgagg cgcgcttgag actgtgcatc ccgtgacagt cgttgcagga
     5041 ggcggccgac ccgatgcctt tctggatgcc gcgggcgtga atgccgcgca tgtaacggtc
     5101 aaaggagtcg gcgatgcgga catccggatc ccgcgtgagc gtctgcttgc tatggcaact
     5161 gctgcacgta taggggagtt tccccgccga ggtcggtgct tccgggttgg tgtcggacag
     5221 aatgttgtgg tggctgtggc aggaggcgca ggtgggggcg ttcggattgc tggtggcatg
     5281 ggcggaggtg gcgtacaact cggcgacgtc gctgtggcag gtgccgcaat cgaccggctg
     5341 caattcctcc gcgtggggat agtcggcgtt gcccgcaaga tcggtgtggc agtcgataca
     5401 gctcatgccg gcgtggaccg aggagtcgag cgagcccggg gccacgaaca ttgagatctc
     5461 ggcccctttc cggttcaggc cggtgagttc attatcggag tgacagctca ggcaggtttc
     5521 gttgtcatcc tgagccgata ccgccgcggg caggcacaga agcagcgtga ccagcaggag
     5581 cagggaaagg cgataccggc gggagcagcg tgcgttcatg tttgacctct tcgactagtc
     5641 aggtcctttt tgtgatatat ttcacgaaga aatatacgga tgtgtcgatc aattttcaag
     5701 agaattaacg gtagccgaag atttcggcat aaatgattaa caggattagg attaacccga
     5761 tggtcagggc gaccgcgccg aaaaccttcg ccgtgcgcac catcaccggc ggaagcggcg
     5821 gtacgagatg gtctttgatc ttgcgggact ggatcagctc ctcgtactcg cgggggcggt
     5881 cgttcttgag ttcctcgacg gggacgcggc cggtgaagat cacggtgtcc atcgggaatt
     5941 tgtcgggccg gaagtgggtg ttgaagaagt ggacggtgaa gatgaaggcg acggcgagga
     6001 gggcctcgtc ggagtgcacg atctgggcca cgttgatgat ccagccgggc aggaagcggg
     6061 tgaagaattc ggggaaccag agcatgaggc cggtggtgcc gatgatggcg acgccccaga
     6121 agacggcgaa atagtcgaat ttctcccagt aggtccagcg cccgtagcgg gggcgcggtc
     6181 cccggccgag gaaccacttg atggtggcga ttgcctcgcg gaggtcatcg cggttcggga
     6241 gcatcgagtt tttcgtgaac aggtattccc gccatgattt ccgctgccgg cgtttctcgc
     6301 tgatgagatc ccagaggtgg atgccgaagt aggcgaaggt gacgacggcg gcgacgcggt
     6361 ggatgtagct ggtcgactcg aacccgccga gcagttcgct gatccaccgg gcccagccga
     6421 ggtaggagaa tttcagcgtc atgccggtga tcgccagggc gatgaagctg gtcacgacca
     6481 tgatgtggag ccggctgtgg aggggcttga agcgacggta ctcgagactg ccccggtact
     6541 gcgtgcggac ctgtttgtgt tcgcgcatca tctgccacga tttcggcagc cagaggatgg
     6601 tatgggcgcc ggcgacgatg agcgtgccga ccagcagggt ggtcatgaac cagaaggcgt
     6661 agaagaggat ggggtacttc gtgcggtcgt ggtgggtggc gtgggtcagg tagccggcga
     6721 actggcggtg ggaccccttg tggcactggc cgcaggtttc gacgatgtgg gcgcgggaga
     6781 ggtgcgagcc gggatcgctc gggggcagag tgtcatgggc gccgtggcag tcgtagcact
     6841 gggccgcggc cgtgccgccg agctgcgaca ctttgccgtg gacggtctcg aaataggact
     6901 cggtgacgtc ggcgtggcac gcgccgcact gcttgcgcac ggtgaggcgg aaatcggcca
     6961 ggtcggcgcg ggtaatggtg tgggagtggt ggcagtcgtt gcagatcggc agcgggtctt
     7021 tggcgtccgg gttggcggtc gccgagtgaa tgctcttcgc gtactgctcg tagatgccgt
     7081 tgtggcagcg ggcgcaggtt ttggggatgt tcgcgcggtg gacgctcgag cgggcgtccg
     7141 tctgcggcag cacgtggtgg gcggtgtggc agtcggtgca catggcggtg accaccaggc
     7201 cgctctgcag cagccccttg ccgtggatgc tctcgatgta ctcctcgacc cggtcatgcc
     7261 cctcgttgat gcgcgcggcc gcgcgcgcgc ccgcccggtg gcattcggcg cacaggcgcg
     7321 gcacgttggt cgggaaagtc cgcgcctgcg ggtctttccg cccgagcacg ccgtggaccc
     7381 cgtggcagtc gatacactcg ggggcgtcgg gatcaccccg gtcgaccaac tggccgtggg
     7441 tgctctcccg gtaggtggca acaacctcgg cgtggcagat ggagcagtcg accctgttca
     7501 cgaccgtggc gcaggggcgc tcgttgtgcc ggggggtcac gccggtgtgg cactgggcgc
     7561 aggtgacgcc ccggtggacc gagctatggg tttcgaggct gtcgaccgag atcgagacgg
     7621 tctttccccc gcgcaccatg gtcaggttcg gccggccgtg gcaggccaaa cactcccggt
     7681 ctgacatccc ttcctggtag aagacgcggc ggatctggtg gggggaatgg caatcgacgc
     7741 aggcggggac tttccccggt tccttctccc acagctcgcc ccggatgacc tgttggtgga
     7801 cctgttcaat ccggccgtgg cactgccggc aagtggccgc gatgttgttc cggtggatgc
     7861 tcgactgggg atcggcgtgg tcgcggacgt tgtgggcggt gtggcaatcc gagcagacgg
     7921 cggtgacggt gagtccctgc ttgaacagcc cgatgccgtg gatcgactcg gagtagtgct
     7981 gcatgatgct gtcctgcggg atgtcgtagc gctgatggac gggcgagcct tccttgtggc
     8041 actccccgca gacgaagggg atgttgaact tggcgacctg ggagttcggg tcggtgcgcg
     8101 ggaggatgtt gtggccgccg tggcagtccc agcagcgcgg ggcgagctcc gcgccttcgt
     8161 ggacgagccg gccgtggagc gactggtagt agattttcgc ggtggtttcg tggcagacgc
     8221 cgcagcgcac gccgccggtt tccgggtcgg agtccgccgg cgagagaatc cggtggctgc
     8281 cgtggcaatc ccagcactgg gcggcggtcc cgtgcgcgct cgtgcggaga gcgccggcgt
     8341 cgtggcagtc gccgcaggcg gccggcgcga gatcctcggc gtggggccag tcctcgaccc
     8401 cggcgaggtc ggtgtggcac atgatgcact ccagctcggc gtggaccgat ccggcgaggg
     8461 catcctcccc cacgtgcatc gaaatttccc gcccgtgcag cgtcccggcc agatcctcgt
     8521 cggcatggca ggacagacag gtttcgttgt cctgcgccag agcagggagc gccgggagca
     8581 gcgcgagcaa caccaacggc agcagtcgtc tcataacggc atcgcggtcc ggcccgcggt
     8641 caatacccct gatccgcggt gtggcaggcc gtgcaattca cctctctcca agcctcatcg
     8701 atatcttccg ggtggcggaa ttccagtccg gccagattca tttccaggct gtcgatggcg
     8761 gtcgagggtc cctgggcgac aatgaggtgg caggtccggc agtcgttttt catcggctcg
     8821 ccggcggcgt tggtcatgct cggcgtgtgg caccggaaac agccgtcgct cacgaagtgc
     8881 gagaggtggt tttcccggac gcggtaatcg gtcttcatct ccgggaagaa attgagggta
     8941 tagatttcct ggaggacggc ggccgcccgc tcgatgtcat ccttctgcgc ctcgacccgg
     9001 tcggggtagt tggttcggta gtactcgcgg aggccttggg caatcgcctc cagcgcctgc
     9061 ggcttggtct tgtattccgc attcaggagg tcgacggcaa gcttgcggat ggagggcagc
     9121 tccgcggaga gctcatgcct gttcagggcg cggttgacgg cggtggcggg cgggttgaac
     9181 cggtgggagg ggcggttgtg gcaatccatg cagtcgaacg cccggatcgc atcggcggag
     9241 tcggcgaccg ccggctgctc ggcatcggcg ttcacccaca cggtcgtgtc gccctccggc
     9301 ccgatcgagc ggacccaggc gatcacgctg cgcttctcat cggcggggat gtattcgagc
     9361 cttcgttcgg tcagcatatg gtagtggatg ccgccggcct ggccggtagc ccgcggcgtg
     9421 ccgccgccga ttttgacgcg gagggccacg gtccacggcg aattctgctc atcggtccgg
     9481 tagtaggtgc ggacgaggag cttgtcgccg tagaactgct ccggccagtg gcactgctcg
     9541 caggtttccc gggccgggcg caggttcacg atgggcgtgg gaatgggttc ggggtaggtg
     9601 ttgagggcgg tcgcccacac ctgccgcaga ccgtccactt tcgacttcac ccagaacgag
     9661 gcgcccggtc cgatgtggca ttcggcgcag gccacccggg catggggcga gttgtggtac
     9721 gtcacgtact gcggctccat cacccggtgg caggtttcgc cgcaaaaaac caccgattcg
     9781 gtggcgtcgt acgcctcggt gccggcgtac acgaccacga gcacgagcac tgcggtgaaa
     9841 gcgaggaaca gccagaggtt gcggatgtat ttcgggtcgg ccggatcgat ggagagggtg
     9901 aagcggacgt cctcgccggc gcgccgggcc cggccgatct gcagccgcac cgaaatcaga
     9961 aacagcaaca cgccgaggag aatcaccgcc ggcgaaagga tgaacgtcac cagcaaccgg
    10021 tacatgttct catgggcggc ggacaagtcg atcaggacga gaatgataaa gagggcgccg
    10081 ccggcaagca cgagcgcgcc gccgagcgcc gccaggggat ggcgcaagag tgttcgtctg
    10141 ggatggccat tcgtagtcat tagtcgatta cctcgttatc gtttgcgact ccgatcggcg
    10201 ccaacatacg gcaaaacggc cggcgaatca agacatgcag gctcatattt cgccgcaatc
    10261 agcactttcc cgaaagcgga ccgcccagtc cgcccgatac gccccgcctg gtcggggtgg
    10321 cggaccgacc ggtttgtccg gtcggtccgc acccgcgcca gatcagaagt tcaccagcac
    10381 tcccgaccgg accatatagt agcggccggt ctcgtccccc cagacccagc cctggtagtc
    10441 gaccacatgg gcgacgtccc cgtcgagcgt gaaggtgaaa gtcggcgtga attggtactc
    10501 caccccggcc gcgaaacgga ggatttcgaa gtcgaggtcg gagtacgcat ccatctcggc
    10561 aaacgagaag tccatgtgtt cgagcacccg atcgccgtcg tagaaagcgc gaccgtcggg
    10621 cataatcccc tcctcgaacc cggctttcga caggttgtag gtgagggtgg aggtcagccg
    10681 cattttcgcg atcggctggt aggcggcgct caggtagaac gtgtgagcat tggtcgtgta
    10741 gccggccatc tcgtacgtcg tgccgtagtg gaagggcgcg atgacggagt cggccgattc
    10801 ttcggagaca cgcacggtgt ctgtaaagag gtggcctgct cacccgtcct acagggcgac
    10861 cgacaccggc cccgtggaac ggtaatgatt gaacgtgtac ccgatagtcg ccgagaagcg
    10921 gtcgtccggc gtgcggatga cgttcaaatt cggggcaatc gcgaaccggc tcacgtcggg
    10981 gctgttcaga gctggaggtc gatccgggtg agcatttcgc ggcggctgac gtggcaatcg
    11041 acgagcgagt cgaggatcgt gtgggtgagc attttcccgc cgagggtgtc cgccccggcc
    11101 gcccactcgc gggcggcgaa gtcgagcagg cggtcctggc cgagctggtg gatgagtgtc
    11161 cggcagccga agtcgagatc gagagtgcgg ccattgacca gactcatcct ggcctgcagg
    11221 ttgtccttat caaagaaatg cccgttcgct gtcaggagac tgttcccacg gcgggagagg
    11281 agcctgagcc ggacctcggc ccagccgtcg tcctcgatta tgcggtattc cccggccttc
    11341 ttgccgtcgt taaggaactc cgagtagtgt ccgcccagcc aggtcgagta ttcgtcgtac
    11401 gactcggcct cctcctgggc ggcacgcggg gcggcgacgg cgagcagggc gaggcaaaga
    11461 acagatgtta tcgcagaggg tcgcatcttt cacctcctac ctggtcaggc ccgcgccgcg
    11521 ggcggacatg gattgtgagg gaaggtcgct gccgtgaatc tgggtgtggc actgcgtgca
    11581 cttggtcagc atgccgatcc tccgcgactc gcgggtcgag acggtgcgcc gttccggggc
    11641 cgcggggggc tggaaggttc cgccgatgcc ggggacggcg gcgtggaagt gcatggggtg
    11701 gcagtcgagg cacagagccg gctcgttctg tttgagcaga ctgttggcca ccgtgccgtg
    11761 cggggagtgg caaatcatgc agttttcctg aaccggggag tgctcgtaga tgaagggccc
    11821 ctccttctcg gcgtggcagg agaagcacag ttccccgttg gtcatgtcct tcacgaacat
    11881 tgtcccgccg ccgtggacgc tgtggcagtc gagacaccgc atcttgcctt cggcgatagg
    11941 gtggtgggac ggcatgtagg cggcggcccg cacctccgcg tggcaggagt agcacaaggc
    12001 ggggtcgcgg agccggtcgt cggtgtactc ggcatggatg cggtggcagg atgcgcaggt
    12061 gacgccggcg gcgtagtgtg cgctgaacgc ccagtcatcg aactggtggt tcttgtggca
    12121 gttcaggcac agcgccgcgg cgccgaactg atcggccctg gccggattga tgatgtccgc
    12181 cgggtcgccg gatgcgacgt ggtcgaccgc cgatccgtga catgcttcac aagtgcccgc
    12241 ggcgagcgcg ggatcgccgg cgaaatatgc gccgtggggg gacttgtgga acatgtcaac
    12301 gatgtcgtgg cagccggcgc acgcgctgtc cgggacttcc gccacggccg ggccgaccag
    12361 gagcaaggcg agcgccagcc cggccgccgc cagcgcggcc cggggtatcc gcagtcctcc
    12421 tgaaatcaga caacccatga aacctcctct gtttgttcgt gctgtgaaac cccttggcag
    12481 tttatttttg ctttgcgtgt ttccctgccg gtctgcggac gctcctgggg acggcgacgg
    12541 agagggggcg tgctgatgcc gcaggcgagc ggtttcgtcg tcttgccgcc accaccgagt
    12601 cggacgaaga ggttgacggc ttcgtcggga atatccgctt ttcggtccgt caccgccgag
    12661 tcaccaatcc ccgcgaattt gtcagtaatc acgagccgcg gagagcggac gggccaagtg
    12721 aaaaagtacc gtacggccca tcttgttgtg acttttttca cgaaaacgcc gggcgcggcg
    12781 gttatcttgg atgggcagcc gcggcggcgc tcccccggcg caaagcgctt ggcaacccgc
    12841 cgccgctcgc cgtataatgc ggccacggag acgagccgcc tgaaaagaaa cgctgacaga
    12901 gaatgaggta cgccatgcac gacctggttg cgcgcctgtt gcccgaagtc aacgagatca
    12961 ccgacccgac actgcgggag aagacgatcg cctgctgggc ggatgcgatc gcattccgcg
    13021 gctggaccga acacctcctg cggaacatcc cgttcacgct gctggcgcag aatgtcaaaa
    13081 tcacctttgt cgaccatgtc cgggcggtct gccgcatgtg tctggcctgc gacgacgtgc
    13141 tgaccgaagt gcacggggcc aaccgcacca gggttaaccg cgattacctc atcgcggggg
    13201 cgctcctggc ggacgtgggc aagctgatgg agtacgagat tgtcgacggc aagccggtga
    13261 agagcgacta cggcaagcac ctccggcacc ccttctccgg ggtcggactg gcctttcggc
    13321 acgggctgcc ctcggaagtg atgcacgtca tcgcgacgca cagcaaggag ggggagggtg
    13381 aaaagcggct gccggagagc atcattttcc accattgtga ttttatcgat tttgacctgg
    13441 tgaaataagc gtggggggga gccgcggggc tcccgcctgc cgaacgggaa aacgaaggcc
    13501 cacgtcataa aggatcgccc catgtcccat tcgcagactc tcgttgagaa gatcgcccag
    13561 cgctacgcgg tcgggctcga gcccggtcac gcggtccgcg cgggcgacta cctctcgata
    13621 aggccggccc acgtcatgac gcacgacaac acgggcgccg tgataccgaa attccagtcg
    13681 atcggcgcgg cccgggtggc caacccgcgc cagccggtgt tcacgctcga ccacaacgtg
    13741 caggacacta ccgaatcgaa cctcgccaag taccgcaaga tcgagtcgtt cgcagcggcg
    13801 cacgggatcg atttctaccc ggcgggacgg gggatcggcc accagatcat gtgcgaggaa
    13861 ggatacgcct ggccggggac catggtggtg gcgtccgatt cgcactcgaa catgtacggc
    13921 gggctggggt gtctcggcac gccgatcgtc cgcaccgacg cggccgctat ctgggccacc
    13981 ggtcgcacct ggtggcaggt gccgccggtc gcccgctgtc aactcaaggg acaacttccg
    14041 ccgggtgcga ccggcaagga tgtgatcatc acgctctgcg gctatttcaa caaggacgag
    14101 gtgctcaacc acgcggtcga atttgccggc gacgggctcc gctcgctgtc ggtcgatgag
    14161 cggctggcga tcgccaacat gacgaccgaa tggggggcgc tggtcggcct cttccccatc
    14221 gacgacgtca ccctggactg gctccgccgg cggatcgagt ttgtgcggcg gcgcgggctc
    14281 gccggcgtgc cctcggacgc cgacggcggc ggggagcacc cccggctccg cacggcgcgg
    14341 ctggaggacc tccaacgcca catcccggcc gccgatccgg gggcgttcta tgccagggag
    14401 ctcatgctcg acctcgccac ggtcaccccg cacgtctccg ggccgaacca tgtcaaggtc
    14461 atgacctcgg tggccgacct ggcgcgtcag aagaagcgca tccacaaggc ctatctcgtg
    14521 tcctgcgtga acagccgggt ggaggatctc gcggccgcgg ctgcggtggt gcgcggaaag
    14581 cgggtggccg caggggtcga gttctacatc gccgcagcct cgtccgaggt gcaggcggag
    14641 agcgagaagc ggggtgactg ggcggctctg ctcgcggccg gggcgatccc gctcccggcc
    14701 ggctgcggac cgtgtatcgg tctgggccgg gggctgttgg gcgagggcga agtcgggatc
    14761 tccgcgacca accggaattt caaaggccgc atgggcgcgc gcgaggcgga ggcctacctg
    14821 gcctcgccgg cggtggtggc cgccagcgca atcgccggct acatcaccgg accggccgag
    14881 gcgacggcac ggccgaccac cggcgagatg cgggtgaacc gaaaacccgc cggagtaacc
    14941 aaggcggcgc gggtgatcga cggcttcccc acccggctgt ccgggcggct ggctttctgc
    15001 cgccaggaca acctcaacac cgacgggatc taccccggca agtacaccta tatcgacgac
    15061 atgacgcccg agcagatggc ggcggtggtg atggagaact acgatcccga attcggcaag
    15121 ctcgtgcgca agggagatat tctggtcggc gggttcaatt tcggcaccgg ctcctcccgg
    15181 gaacaggcgg cgacggcgct gcagcaccgc gggatcgcgc tggtggtcgc cggctcgttc
    15241 tccgagacct acaagcggaa cgccctgaac aacggttttc tcgcgatcga atgccccgac
    15301 ctggtgaacg atctgaaagc cgcgtgcgac ccgaaccggc tgacggcggt ttccgacagc
    15361 gaggcgacgg tcgattttgc ggccgcgtcg ctggtgttcg caggcaagac ctacgaggtc
    15421 gatccgatcg gcgcggcggc gcaggagctg gtgctcagcg gcgggctgga aggatgggtg
    15481 cggcagcggc tgtaggagag aacggccgag agacggcatt ggccaacccc cacgcggagg
    15541 aatagacatg gcgctcaagt atcacagctc agtcgcgtcg gcgctggccg cgcttattgc
    15601 cgccggcccc ctgttcctcg cgtgcggttc cgatgacggc aaccccttcg gcggcggcgg
    15661 gccggaccat gccctcaacc cggccgagta tttcggcctg gttccgggcc gaaccataac
    15721 agccgggtac gtgaactatg tcggcgacag tctccgccag tttgtgccga acagcccggt
    15781 cgacgcctgg tccttcaccc cggtcatagg tctgccgacg atctgcaacg gtcggctggc
    15841 ctacccggtg tccgccgcgc ccatcccgag taactattcc gacccggatt acgctttcac
    15901 gctctacttc agcgccgact ccagcggcct gtactggcac ggcggctcgt actgcggact
    15961 ggaccttccc ctgccctcgc ccggcaggat cgccccatac ccgctggcga gcggattgaa
    16021 gtggcagaca atcggccatg cggagaccga catcgaccag tgcaaccggt cggtcgccga
    16081 tacgctcaac gcaacctgtc gacggattac gaacgccgtc gtgcgcggga atgcctaccc
    16141 ggaggtattc ctgatccgaa taccgttcat cgacgtgccg gggcatatcg ggacgcgcta
    16201 ctacgaggcg tggatcgccc cgggctacgg tccggtgcgc ggcgcgagct ttgatcaggc
    16261 gtacgagcat ccggacgagg ggttcgagtt catttcctac tccccgtgag gctccccggg
    16321 gccgattccc cggcgccggg acgatagctt tcagccggcg cgggcagcgg cccgaaaggc
    16381 aaggcgatgg actggctcat catactcgtg ttcggcgtcg tgtacctcgg gatgatcctt
    16441 gggcggattc ccggtctggc ccttgaccgg accggggtgg cgcttttggg ggcgctctgt
    16501 ctgctggggt tcgggcgggt gacgacggcc gatctgccgg cggccgtgga catgtcgacg
    16561 atcgcgctgc tgtttgcgct catggtggtg tcatcgcagt ttcggctggc cggggcctac
    16621 tccttcctgg tgcggcgagc gggggagctg gcggtgtcgc cggcggcgct gctggcggtg
    16681 gtgatcgggg tgacggcgct gctgtcggcc gtgctggtca acgacattgt ctgtctggcg
    16741 gcgacgccgg tggtgatcga ggcctgcgcc caccgccgcc tgaacccggt cccgtacctg
    16801 ctggggctgg cggcggcagc gaacatcggc tcggccgcga ccctgatcgg caacccgcag
    16861 aacatcctca tcggggaggt cctccgcctg tcgttttcgg ggtacctgct cgagagcctg
    16921 attccggtgg tgttcgggct cgtattgctg tggctcattc tcgcctggca gtaccggggc
    16981 cggctgggag cggagacggt ggcgccgccg gtcgcggccc cgctgttcag ccggtggcag
    17041 acggccaagg ggacgctcgt gctggcggcg ctgctggtga ttttcctggc cggcagttgg
    17101 ccgcgcgacc tggcggcgct cggcgcggcg ggagtgctcc tgctgtcgcg gcggatgcag
    17161 tcgcgcgaga tgctcgggct gatcgactgg ccgctcctgg tgctgtttct cgcgctcttc
    17221 gtggtcaact acgcggtcaa cagcggcggc tacatggccg gcgtcatggt cggactggac
    17281 gcggccggca ttcccctgga ccacccgggg tggctcttcg cggcgagcgt cgtgctgagc
    17341 aacctggtga gcaatgtgcc cgcaaccatg ctcctgttgc cctcggcgac ccatcccctg
    17401 ggcggagcgg tgctcgcgct gtcatcgacg ctggcgggga atttgctcgt ggtcggctcg
    17461 atcgcgaaca tcatcgtgct cgaccaggcg gagcggttgg gagtgcgcat ttcatggcgg
    17521 aggcacgccc ggacgggagt gccggtgacg gcggcgacgc tgctggcggc gggggtatgg
    17581 ctgtggctgc gggcgtagtc tctcagaaca cctcggcgct gaaacgatag atctcggcgt
    17641 tcttgtcttt ccagctgctt ttcggcagcc cggctttcag gcacgtttgc ccgaggaatt
    17701 ccatccggtc ccagccgttt tccgcggcga cctgcgggag gagcagcccg gagtgccagt
    17761 cgagtttgat catgaggccg tcgcggccga cctggatctc ttcgatgtcg cgcacccgcg
    17821 cgagcggcga gaggatcgag atctcgatat cgatacggtc aagctcgcgt tcgtgcagtt
    17881 cggggaaccg ggggtcctcg aacgccgccg ccaccgccat gtcggcaatc gtccggggca
    17941 gcggctcatc ggagcggagc cgcccgatac agccgcggag ctgtccctgg aggtggaggg
    18001 tgacgaacgc gcctttctgc atggccagcg actcgggagc gggcggggcg tactctcggc
    18061 cctccaggcg cgcgcggatc gcgtcgcggg cgaccgccgc cagcagccgc cggtcgtcat
    18121 cggcgatgcg ctcctccctt tttcgcggtt cgccggcggc ccgggcgccg atgacctcgc
    18181 gggcggccgc tttccgctcg gacagaaaga cggccgagag gtagccaacc acctcgttga
    18241 aatcgccggt cgtctggccg gagtgggagt agtcgatcac ctgggcccgg gtggcgccga
    18301 ggcgccgccc ggcgataatg gccgcggcga tgggcccgcc cccgcaggcc tcgcccttcc
    18361 ccgtctcgag cgtctccagc aggaacgcct cgtcgaactg ctcgactgcc tgccggacgg
    18421 tgccgtcgag acgggaggcg actttcgcgt tgtggaaatg ggacaaatcg ctggaagcga
    18481 ccatgagcgc gttggcccct ttgagcgcgg cggcgagggt ttcgccgagg atgcggaccg
    18541 actcctcctc ctggtcgccc atcacgatcg cgacgagctt gaagcggccg agcatgacct
    18601 ggagaaacgg caactgcacc tcgagagcgt gttcgccgcg ggtcgagccg gtcgcgtggc
    18661 ccatgttgga gtagtaaacg ttggacgggt tgatggcggc gattcggtcg gccagcgggc
    18721 ggtcggtctc gaccacgccg agcggcgtct cgtagccggc gccggcgaag accgatgagc
    18781 ctttgaagaa gaccgtgtgt gacggcgaga tgaccacgac cgtgtcgaac tgctccccct
    18841 cgagctgctt gtaggctttg gccgcagtca ggcccgagta cgggtagccg gcgtgggggg
    18901 cgacgaccac taccgggtgg ccggtgatta tgcgtttgcc cgcctgggca tagagtcggg
    18961 cgatggcggt ggccagctcg cccgggtcgc gcgggtagaa ggcgcccgcg acggcgggct
    19021 tgcgtatggt cgcacggtcg gtttccatgg cgatagtata cgccggggaa ggcgggtgtg
    19081 tcaaatggag gggggcgagc cggtccaatt aaatcggctc agccggcgac ctcttcggta
    19141 tcgaccgggg cgatcgcggg ccggggcgac gccgttttgc cgccgccgtt gggccgcgcc
    19201 ccttcgatga gcgccgccag cgccccaatc atcccgctcg cttcgtacgg caggaagatc
    19261 ttgttcgcct ggttctcggc cagtttcggc agcatctcga ggtatttcag catgatcagc
    19321 ttgtcgtcgg gccggctggc gtggatggcg ccgaagacgt tattgatggc gagcgcctct
    19381 ccctcggcca ccgtgatctg gcggtagcgc tcggcatcgg ctttcatacg gacggcctcg
    19441 gcgaatccct cggcttcgag gacggcggcc ttgccgttgc cctcggccag caggatggct
    19501 gcctggcgcc gggcctcggc ctcgaggata gcggcccgct tgtcgcgctc cgctttcatc
    19561 tggcggctca tcgcttcggt gatgtcgcgg ggggggtcga tgcgctggag ttcgacgcgg
    19621 ttgatgcgga cgccccactt gtcggtggcg tcgtcgagga catcgcgcaa ctggccgttg
    19681 atcttgtcgc gggacgagag gcaggtatcg aggtccatct cccccatcac gttgcgcagg
    19741 ttggtctggg ccagcttgga ggcggcgtcg aagtagttgg agatttcgaa agtcgcccgc
    19801 accggatcgg tcacctgcgc ccagatgacg gcgtcgacct cgaccacgac gttgtccttg
    19861 gtaatgacct gctgcggggg aacatcgagc acctgctcgc gcatgtcgat cttccggaca
    19921 tcgtcgagga gcgggaaaat gacgttgagg ccgggcgcga ggacgcgttg gtatttgccg
    19981 aggcgcacga ccacgccccg ttcgtaggga ccgaccactt tgaccgagaa cgagacgatg
    20041 accacggcga aggcgatcgc cgcgagcaga aatatcagga ttccggtatc catggggctc
    20101 actcctcact ttcctcggct ctttcaacgc gcaggcggtt cccctggacg gaggcgaccc
    20161 gcacgcgctc ggcgggggcg aaggttccgg cggcgacggc ccgccacgtc tcgccgtcga
    20221 ccacgatctg cccggggctc tcgcgatcga tcggtttggt cacgatgccg atctgtccga
    20281 tcaggcggtc gatgttggat tgcaccggcg agggtttggt gatgcgccgc gccagggggc
    20341 gcatcagcgg caggagcacg agcgaggcga tgatgaacaa ccccacctgc cagtagtagc
    20401 tttcgggatt gaactgggcg aagacgccgg ccgcggccgc cgcgatggcg aagcagatcg
    20461 cgagaaacga cgggctggtg atttcgatga tcagaaaaat gactgccgcg gccatccaga
    20521 tccagaacat ggtcgccata gaagctcctt tgcgcgagtg tcggcgcggc cgctgtcgcc
    20581 cggccgcaac tggtcaaagc tagccacgag gcggcccggg attcaacaaa aactccggtt
    20641 cgccggtcgc cccggagggc gtaaacgcag ccggcgggag ccgccgtcgc gcgccccggc
    20701 ccggcgggac cgggggggta cgctcgctct ctgccggatt gaccgcatcg ggaagcagaa
    20761 tcgacagccg cagttgccac gatgggagac ctgcggcagg ccgctctccc accgcgagag
    20821 caggtgcttg gccgagcgcg tgcgaccgaa tgggtacatg ctttgattac gtgcagagtg
    20881 aaaacaacaa cagccataga tgacctagct gaggcaaacg caaggaggtg ttgcgaaatg
    20941 acgaacgtgg cgagacgtga aactaacggc atgcggaatc gcctgcccag cccgttcttc
    21001 gaggacttct tccactggcc gccggcgccg tgggagccgc atcactggcc gcgggtcgat
    21061 gtccgggaga cgagcgagga cgccaccctc ctgttcgagg ccccgggcat gcgcaaggag
    21121 gatctcgaag tgcaggtcgg gaacaaccgc ttgacggtgt cgggcgagcg gcgcctcgag
    21181 gccgatgact ccgatgaggg gtggggtccg ccgcgagatc gcgaccggga cattcaagcg
    21241 ccagtatacg ctcccgtcgg ctatcgacca gcactcggtc aaggctgagt acaagaacgg
    21301 cttcctgcgg ctgacgcagg ccaagcggga ggaagcgaag ccgaagcaga ttgacgtgaa
    21361 agtcccgtga cgatgcggtc tcctcgctct gcactgtgtg acccggccgg cccgatgggc
    21421 cggccgcgtg ttttcgcgtc aggacgcagg gtctcccggg acgcgcagag cggcgagacg
    21481 cgccagaacc agcggcgcca gcggcatcat gtatttctcc cacgccagat acgaaaaggg
    21541 catgacgagc aggaaagcga tcacgccgct gtcgagcagg agtacgacat cacccgtccg
    21601 ggcgcggatc cgggcgactg cgctctgggc gatgtgcagc acgatcggca acccgaagag
    21661 gaatccggca aaaaagaacg cgtgcggcag ccacggagcg gccggaaagt tttcgaccgc
    21721 cttgtgcagg agaccgaccg tctggacgcc gatcgccttg gcggcgggcg aagcttcgac
    21781 cggaaacagc cagtaaaggg ggctgagagc cgccgccgca atcagccgcc cgcgcgtgta
    21841 gacccgccgc ccggcatagg ctgccgccgg aagcaggtac cagaagagca ggctgatgta
    21901 gagcaccagc gcggaagggt gaaaggcgag gcctccgccg aggtagagcc cgcgcagacg
    21961 gttgtcgggg ctgacatgcc cccacagcag gaacagcgtg agcagcggaa cggccgccgc
    22021 cgcggccgcg ccgagcgccc gcccggcaaa ttcccttcgc tccggagaat tcctgagcag
    22081 cagcgcccag gccgcaaccg ccatcaccgc aaataccgca tactgacggc acagcagagc
    22141 tcccgcgagg ccgagcagga tcagcaccgc ccggccggcg accgctcccc acagggccgc
    22201 cagcagaaac aggatggccg cgccatcggt gaagacgaaa atcgaaaaac cgatcatgta
    22261 cggctgcagg gcgaacagga gcgcgaacag aaaggcgagg cggccatccc cgcacatccg
    22321 ccacagcagc cggtgaaaca ccaagtagga accgagggca atcacgagcg acagcagacg
    22381 gaggttccat agaccgaggc cggcgagatg cccccaggcc gcatagaggg cgaagggcag
    22441 cggcccggac atctcgggat aggtgcgcag cgtttcgaga ctgggacggg aggcgaagag
    22501 gcggaccgtg tcgacgaagt ggctctcgtc cccccagggc ggccggtcgg gaccggcgac
    22561 gatcaccgcc gccgcctgaa ggagaatcag gcacgccagc agagacgcca gggacttcct
    22621 gtaggaccat cgcggtactg acacttcaca gctccggcgt gatggggccg acggggggaa
    22681 agatcggccg gcgtgcggga cgtgtcaacg ttgatcaggt cggccgccca cggcgaaatc
    22741 cgcaccggga cccacggcct gaaagggaga tgggcgccca cgcggccgcc cggggcggcc
    22801 ggatcagcgc aggcagaaag tgcgcacgag gttgaatgcg cgctcgcgga ccgacacgcc
    22861 ggggtagtgg cggctttcga cgaccgctcc ctcggcgtcc aggtactcaa ccgtcagatc
    22921 gtacgccccg ggggggacga caaagtcgcc gacgaagatg cggccgggca ggagtacggc
    22981 cgagcggaga tccgcctgtt cggaaacgtc gtaggcgacg tcggcggcca gtttgcccag
    23041 ccacccggcg atcccgccgt tgtcgacggc gtttttcagt ctggaggcgg agagggtttt
    23101 gagcacgacc cgcacgacgg tgcgccagag aatgatcgac tggcgggctt cgaaagaggc
    23161 ggccgcgacc gccgcgacat cctcgagcaa ctgcagctcg ccgagcaccc ggccgcccga
    23221 ggagacccgg acagccgcga tttccgacgg tctcgggacc agttggggga gcgacagttt
    23281 cgcgtagaag tcgccgatcc cctcgggcag cgggtagttg gcgaacaccg tgtgctccgg
    23341 gcgggcgtcg tcgtagatca ccgtcagcag gtgcaggttc tcatcggtgc ggacgcgcaa
    23401 attgagcggc tgcttcaccg gcgcgagacc caccagcccg accacgctga gcacgccgcc
    23461 gctgtccgca gcgtaggcga ccggaggcgg agggaacgcg tagatgttgg gctggcgcgc
    23521 gaaggcatcg gccagcgcct gcgagtcgat ccgcgcgtcg tcgtcgagcc cctcggcggc
    23581 gtacatgtgc atcgacaggt agcgggcgaa agcggagctg ttgaaacgca catccgtgcc
    23641 cgcgaaagcg acgcaggcgc cggtcgtgtc gcgggcggcg tcggccgcga ttgccgcggc
    23701 cgcatccgcg tacttctgtt ccagcaggtt gagcttgtcg tgggcgcggc ggatttccac
    23761 gaacgcatcg tcgtgcttcc ccagggcaag gtagttgagg gccatgagga ggttcgtgta
    23821 gagaacttca tagtcctcgc cggggtactc caggacgttg tcgttgagca gagtgttggc
    23881 gagcaccccc ttggagatac tcttggtgaa gagttcctcg gcggcccgct cggcgaccgc
    23941 gaggcgaagg ttggaggagt caaactggga ggcgtagtgg taggccagac cggcatcgag
    24001 gtagtacagg aagcggtcct tctccttgta ggcccctttc gatcgggcct cctcaacggt
    24061 ggcgacggcg gcggcgtagt tgcccgagcg cagatcggcc tcgagcggcg cgtagaactt
    24121 ctcccggttg aggccggagg agcagccggc cgcgagcacc agcaaaagcg cgcccggacc
    24181 caggatcccc cgccccgccg atcgcggcag gctcaccatt tcgtgccgga ctgggcaatc
    24241 cccttcttga tcttcttcgt cccgatccag actttctcgt tggtctcaat gttgatgagt
    24301 tcgaggtcgg tctggtagaa gacggtgctc cggccctcga ccgcatcggt gatggtcttg
    24361 atcgcgccga gcaggatgaa gtcggcgccg agctcatcgt ggaggagttt gcgggtctcc
    24421 tcggaggcga actccgcctg gtcctgccgc tcctcgcgaa cctcgtagcg ctcatcgtgg
    24481 ctgcccacga agcgcacgtt gccggagttg atcagttcgc gttcgaagtc ggcggtgaac
    24541 acctcggtgt cgatgtgctc gttggttttg ttgcggatgg tgccgacagt gacgaccggc
    24601 ttttcgccgt ttttctcgac gaaattcatc agccaggggc gggcgagcgc atcggcgatc
    24661 atctcctcgg ccacgaggcg cgcatcggtc tcgttccact tgccgctgag gtcggtggtg
    24721 ccgcccggat cgaggcgggt cacagtctgg ccgccgccgc agccgatcgc cagcgccgcc
    24781 agcgccagca caactaacag gtatctcata gtcatctcct ctgccgccac gttgcggcct
    24841 atctggtagt aagggttacc ggcactcgcg gtccggcagg aagccgaccc ggcttccccg
    24901 gaacatcgtt tcatattccg ggacgggctt gccggaaaca agtttttttg tggccggtcc
    24961 gccccacggc atgccgggcc gcccgcccgg ctaccgggag ccgaagatgt agaggacggc
    25021 gtagtaggcg ccgacggcga tgaagacgac tccggtgagg cggcgcgccc agcgggagaa
    25081 ggcgttcacg cggtggaaga cggagccgat gaagcgggcg ccgaaagcga tgagcgcggc
    25141 aaagaccacc accggcaggg ccgtccccag cccgtagagc acgggcatga gaaaaccgga
    25201 ccgctgttcg agagcgagcg gaatcaagct gccgaaaaag agggccgctg agaccgggca
    25261 gaacgacagg gcgaacatca ccccgagcag cccgcccccc cagatcccca gccggtcaac
    25321 ccgctgctcg aggcggcggc caacgccgat tccgggaagg cggagcggga tcaggtcgat
    25381 gaggaacagc ccggcgagaa tcagaatcgg cccgagcacg acgttcatcc agcgctggag
    25441 ccagcgggcc agctcgggga cggcggtgag gctgctgacg atgagcgcgg ccagcgccgc
    25501 gtaggccgcc agccgcccca ccgtgtacag cacaccggac aggagcacca ggcgggggcg
    25561 ttcaatccgc cgccccacat agctcatggc ggcgatgttg gtcgccagcg ggcacgggga
    25621 cacggaggtc agcaaaccca gccagaaagc cgtggcggcg gcaaccgcga acgaatccat
    25681 cagtcggccc cggcggcgag aaaggtccgc agctcactgc ggacgtaggc aaggtaggcc
    25741 tcctgatcgc cgaccagcgt ccagatccgg tcgaggtttt tccagactgt ctccttcccg
    25801 ccctcgaggc gcgagaggat aaccgcctgg ctgaacagct cgtagtcctc gaggaagtgc
    25861 gcgttggcat cttcatcgaa gttgacgacg cgccactgga ggacgccggc ggcgatttca
    25921 tcggggaatc ccccggttac cgcctcctgc gcataagcct cgagcttccg gcaggtggcg
    25981 caccggcggt tgccgtggaa gtacgtggcg atgacctgga ccggcgcttc ggccggggcc
    26041 ggttcgacgg agtcgatcgc ggccgtgtcg gccgccgggg ccgcgggttc ctcggcggcg
    26101 gaggcgggaa gcgcggcgcc gagcaggcac ccgaacagaa gcatggtgaa tgcgaccggg
    26161 ttcatgcggt tctcccttct ggttccgcct gagagtcagg cgaggatttc tttcagtttg
    26221 gccagggacg ggactttgcc cgcgaccaac acccggccgt cgaccgccag cgccggtgtg
    26281 atcataacgc cggcctccat gatcgcccgg atgtcggaca ctttttccac ctcacaggcg
    26341 aggccgagtt cggccaccgc ggctgcggcc agccgggcga gttcctcgca tttcgcgcag
    26401 cccggcccaa aaacctggat cttcttcatg ttgtatcgcc ctttgcctta gagtaatgcg
    26461 ccatagatca gtccgctgag ggtcgccatc ccgaccacca gcaggacgta caccacggtt
    26521 tttttggtcc caatcaccga gcggatcacg agcatgctcg gcagcgaaac ggccgggccg
    26581 gacagaagca gggcgagcgc cggccccggt cccatccccg agccgatcag cccctgcagg
    26641 atcgggatct cggtgagcgt cgcaaagtac atgaaagcgc cgatgaccga ggcgaggagg
    26701 ttcgcctgaa gggagttgcc gcccacagcc gcggccaccc aggtttgcgg gatcagcccc
    26761 tcctctcccg gccggccgag aagcgctccc gcggcgagca cgccgataac gagcagcggc
    26821 agaatctgtt tggcgaaccc ccaagtctgg gcgaaccact cccccggttc gcc
//
LOCUS       DAOPOE010000042        26812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2312_length_26812_cov_6.188100, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000042 DAOPOE010000000
VERSION     DAOPOE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26812
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_26812_cov_6.188100"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKM94_07295"
     CDS             <1..386
                     /locus_tag="PKM94_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOD66484.1"
                     /translation="GALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVVVIALKDPV
                     HDKVMSNIAEIRARNGQVIAIATEGDTEIADRVNHVIYVPPTYRLFTPLLSVIPLQLL
                     AYHIAVLRGCPVDQPRNLAKSVTVE"
     gene            complement(807..1832)
                     /locus_tag="PKM94_07300"
     CDS             complement(807..1832)
                     /locus_tag="PKM94_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD66485.1"
                     /translation="MSYVFLALLSAALFGAATPFSKALLSTLDAFQLAGLLYLGAAIG
                     ASGLAFGKRPYTLPWRMDRRNQFRLAGAVFFGGVLAPVLLLFGLKAASAASVSLWLPL
                     ELVATAVLGTLLFRDHLGRFGWFGMVGVLLAGTLLSIGEKSAGVVAGLLIMGACLGWG
                     FDNNFTSLIDGITPAQSTFWKGIVAGTVNLGIGLVSSEYAATAATTAAALGVGIVCYG
                     ASIVLYITSAQNIGATRGQMFFAANPFFGVILSVIMLNDEITVVHVAAGLLLALSLLL
                     LFREQHAHEHEHEALDHEHSHSHDDGHHEHVHESIISGRHTHKHLHKPVVHRLPHWPD
                     LHHRHQH"
     gene            1984..2448
                     /locus_tag="PKM94_07305"
     CDS             1984..2448
                     /locus_tag="PKM94_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HOD66486.1"
                     /translation="MKWITRSKVKVDRVACPWLINRFVDRNAEFIFVPANQAVAEAKK
                     QNAIAFDIPGVEPGHHGRECSFEAILKKYRLTDDPALTLLGKIVNGADTDNQLWNQPE
                     GPGLSAIAEGFRHLGFEDDREINTAEWIVYDAIYACCQEMIRQGRHDGALKE"
     gene            complement(2570..3769)
                     /locus_tag="PKM94_07310"
     CDS             complement(2570..3769)
                     /locus_tag="PKM94_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003395828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD66487.1"
                     /translation="MDFSLTDKQLAVRDMARELARRVVAPTITEYDRRQQMNPEVLPA
                     MREANLLGFCIPEEYGGLGTDYISLGLASEELEYADTSARVILSVHIGLFSLPVLTWA
                     NEAQKRKYLVPAARGEKIATFGLTEPAAGSDAVGIQSTAVRRGDHYVLNGEKMWISLA
                     DVADQFLVFAWTDLDKKRARDHSGLSAFIVERGWEGVSTGSLHGKLGVRAGNTGYLAF
                     ADVRVPAENLVYGEGQGFKIAMFCLDQGRYTVAAGSCGLIRACRDASVAYALDRRTFE
                     QPIGEHQLVKQMIANMEAGYEYCTYLWMKAGWLKNRGVRSTKATSLAKWIACREAEAA
                     AANAVQVFGAYGFSDEYPVERFYRNAKGASIYEGTREIHTLMQADYALGFRSDKQLER
                     DLPEAEQ"
     gene            4009..4485
                     /locus_tag="PKM94_07315"
     CDS             4009..4485
                     /locus_tag="PKM94_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66488.1"
                     /translation="MTEPARVKADLVPFRPEDAEKVMSWIDSGETLQYLCGDDTWPPA
                     EDLLASWQKADAASYLLLADRRPVAYAEIWDRPLEQAAEIGHLLVDPARRGRGYGTLM
                     LRLLVERAAHHPRVRRVVLNFHHGDETVLGCYLKAGFELVGSGPDGEGLRMERPAG"
     gene            4588..5373
                     /locus_tag="PKM94_07320"
     CDS             4588..5373
                     /locus_tag="PKM94_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD66489.1"
                     /translation="MTEYKNIIVRKDAGCLWVTINRERALNALNRETIDELQQLFSFY
                     WTDDEVRVVVVTGTGEKAFVAGADITELAETDLRTGTDYSARGLYLMKTIQNFPRPVI
                     AMVNGFALGGGCELAMACDIRIASDKAKFGQPEVNLGIIPGFGGTQRLPRLVGRGTAL
                     KMILTGEMIAADEAKRIGLVQEVVPAAELTARVQAIVELICAKAPMAIAAAKECVNRG
                     LDVSMSVGCDLEKVSFGQMCATGDKNEGCEAFLEKRKPMFTGH"
     gene            5634..6383
                     /locus_tag="PKM94_07325"
     CDS             5634..6383
                     /locus_tag="PKM94_07325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66490.1"
                     /translation="MQSDAIPLHRLYGDLAWLWPLMSPPAEYVAEAAHWKAALRENLG
                     PGRRCLLELGVGGGHNLSHLTGEFDAVAVDLSEPMLAHSRRLNPGVEHFLGDMRTVRL
                     GRTFDAVVIHDAIAYMLSGDDLRAAFATAAAHLGTGGVFVTSPDYVAETFVDGSIACE
                     TRPFDGGSFTYVEYQWDPDPSDTQTETVFTYIIRRGGKVQIEQDRHLFGLFPLRTWES
                     LMAQAGFAVARRDYPVRDDGQPGWLLIGRKL"
     gene            6399..6878
                     /locus_tag="PKM94_07330"
     CDS             6399..6878
                     /locus_tag="PKM94_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66491.1"
                     /translation="MGITINPVAESDRPWVEEVFRSWGADFVVTRGRKVCPEETEGLY
                     AADERGRRIGLLTFQIAGTQCEVVTLDAFEKFRGIGTALMDRAVALARARGCTRVWLI
                     TTNDNLDAIRFYHRRGLVIAHVHVGAITESRRLKPSIPLLGAYGIPIRDEIEFELVL"
     gene            7011..7307
                     /locus_tag="PKM94_07335"
     CDS             7011..7307
                     /locus_tag="PKM94_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOD66492.1"
                     /translation="MKTFFLFTKLAPSDGKRMRERQSLGRAWLHEVKEKCPDVKFLSH
                     YALLGDYDFVDIYEAPDEETAMKVSMISMDKGAFQAKSVLAIPYARLVELANEL"
     gene            7463..8041
                     /locus_tag="PKM94_07340"
     CDS             7463..8041
                     /locus_tag="PKM94_07340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66493.1"
                     /translation="MGQSALTFHPLTPERWGDFEALFGVRGACGGCWCMYWRLFRLAF
                     SIGKGEGNRRAMQKLVRAGTEPGILAYDGERPVGWCAVAPREEYVRLTAARTLKPIDT
                     QPVWSIVCLFVDKGYRRRNVSTELIRAAAAFACARGARIVEGYPYDYRGRAKHQPPPF
                     VYTGLIQAFERAGFVEVARPSKSRAIVRLTRR"
     gene            complement(8052..9566)
                     /locus_tag="PKM94_07345"
     CDS             complement(8052..9566)
                     /locus_tag="PKM94_07345"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HOD66494.1"
                     /translation="MSRPAPTISLPARTAHALAIALFILLAATGLRIVVVNGAEWLSP
                     EVRALIDRLTPTGNIYRLHLFAGVLLAATGIFYCAYLLFTGEHRRLTDLFHRSEYSFT
                     RKLTYLSMLVLAAAALLTGTSLAAGLYSGGPGYLFNSFVHQWSFRLLVLFAALHTLEV
                     SLSLRGRVACMFSGKPLVGAVRWPALAIAAAVAVAGGTALHVSLGRPPTLACRAAGSP
                     IVIDGYLGEGEWAGADSAIVQTQGGANFLYAAAPVTVKTARDRQRIFFLLRWPDDTRS
                     LNRHLVKTDTGWIPERSVFTGPYGEDIFFEDQAALYFSRSGGCASTCHVGRASRPGRH
                     FTGGDTADVWVWMAVSTNPTAEADDRYWAAPAGESGDGRFFDNLAAGGYRDNLDSILR
                     FPYFVPTHRLFRDWLLYGTPGYEAYDHRADTFPLGHRIPAVLVAPSTGDRGDIEARGV
                     WREGVWTVELSRLLATGSPTDIGFQSELYLGIAVFDNAEKKHAGHLRPLRLVME"
     gene            9806..10387
                     /locus_tag="PKM94_07350"
     CDS             9806..10387
                     /locus_tag="PKM94_07350"
                     /inference="COORDINATES: protein motif:HMM:NF017771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HOD66495.1"
                     /translation="MFATDSATTRRRIEDLRHQLLELRRQVRELRKTVPEEVIGREYV
                     FAGPRGIRITLADLFGDRDELIIIHNMGRACRYCTLWADGLNGLVPHLAGRASLVLVS
                     PDPPEIQQAFAASRGWQFDLWSTGAEFLVDMKMADEKGSPWPGISAFVRRPDGTIVRT
                     AFDYFGPGDDYCAVWHIWDMFPSGAGGWEPREG"
     gene            10550..11362
                     /locus_tag="PKM94_07355"
     CDS             10550..11362
                     /locus_tag="PKM94_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOD66496.1"
                     /translation="MADTLRLMGILAHPDDESLGMGGAFARYAREGVETFLITATRGE
                     RGRFRDLRGEARPPMPVVARAREGELRAAARVLGVREVHFLDYVDGDLDRAEPAEAIA
                     RIVGHLRRLNPQVVVTFAPDGAYGHPDHIAISQFTTAAIVAAADPAFPAPGAPPHAVA
                     KLYYLAWTRAKWDAYQAAFKQLATTVDGVERRATPWPDWAITTVVDTAAYWAVAWEAI
                     SCHETQLAIYTKLADLPPEYHRSLWGTQEFYRAFSLVNGGRKRETDLFEGLR"
     gene            11399..12907
                     /locus_tag="PKM94_07360"
     CDS             11399..12907
                     /locus_tag="PKM94_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD66497.1"
                     /translation="MNSNDRTAARHTPIDLSPEEFRALGRDLVDRLAGLFASLRTRPV
                     TPEAAPGRIRERLGADRPLPDQGSDPAALLADAADLLFEHSLFNGHPRFWGYITSSPA
                     PIGALADLLAAAVNPNVGGWKLSPMASEIEAQTVRWIAELVGYPADCGGLLTSGGNAA
                     NFIGILAARAACAPWAVREEGMRGGRRMIIYASSETHTWLQKAADLFGFGTEAIAWIP
                     VDRFRRMDLARLEARVRTDRAEGHFPLAVVGSAGTVSTGAVDPLRDLAAFGRDQGLWF
                     HVDGAYGGFAAALPEASEDLHSLALADSLAIDPHKWLYAPLEAGCALVRDAEKLRHAF
                     SYHPPYYHFEEEAINYFDYGLQNSRGFRALKVWLALRQVGRAGYVQMIRDDIALARHL
                     FAHLAAYPDLERFTCDLSIATFRYVPADLRAGGGEEAREYLNELNENILERIERGGEA
                     FLSPAMIEGVYALRACVVNFRTTREDIEALPEIVVRVGRAADAARREKTLRR"
     gene            complement(12926..13678)
                     /locus_tag="PKM94_07365"
     CDS             complement(12926..13678)
                     /locus_tag="PKM94_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66498.1"
                     /translation="MSHPPFTVATLRDCPGLESEINRLASDLWPKFMLRDPVVEDNWR
                     LLYERYPEYQLFLLDGEDRRVIAVGNSCPLPWEGKPAELPDTGIDWILTELAARATFA
                     PKNQFALQIMVDRAQLGHGLSGQVVAAMAAIGRARGCRNLFAPVRPNRKGDYPLIPME
                     HYIRWTRDDGLPFDPWMRVHARLGAEIVGVCARSMYIPGSIAQWEAWTGLTFPGSGEY
                     VVPGGLAPVRVDRERDRAEYLEPNVWMRHRLT"
     gene            13837..14289
                     /locus_tag="PKM94_07370"
     CDS             13837..14289
                     /locus_tag="PKM94_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOD66499.1"
                     /translation="MKPVTCRAELVQDDATSGCGITLPFDPKAQFGKVRAPVVATINR
                     YSFRTTVFSMGGCYWIPVNKSNRDAAGIAPGDRFKLRIALDTEPRAVTPPADLKEALA
                     GDPTAKTVWDKLSYTHRREYVEWIAQAKKPETRARRLQKTLERLKEPR"
     gene            14403..14888
                     /gene="bcp"
                     /locus_tag="PKM94_07375"
     CDS             14403..14888
                     /gene="bcp"
                     /locus_tag="PKM94_07375"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOD66500.1"
                     /translation="MAVSSAVTVGRKAPAFALPNQQGDTARLSDYEGKWVVLYFYPKD
                     DTPGCTTEACDFTDNLSRLDKLNAVVLGVSPDSVESHARFVEKHNLKVTLLSDPEKKV
                     LQMYGAFGEKMMYGKATVGVIRSTYLIDPGGRIAYAWPNVKATGHAEKVAAKLAELAQ
                     R"
     gene            15009..15596
                     /locus_tag="PKM94_07380"
     CDS             15009..15596
                     /locus_tag="PKM94_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66501.1"
                     /translation="MIARRSAVPRVLPTLDGERVRLRPLRHDDALAICRWLQDPELSR
                     RLELLERPFTLGTARMWVRIAVAGVGDRTGFFFAIADCRTDDLLGMIALHGVNWHDRN
                     GEIFYWLARPFWRSGLTEDALRLLLGFAFRRLRLHRVWASVVETNRPSTALLEKAGFS
                     REVVRRDARRRGRAWLDMYTYGLLGEEFAPQRRRV"
     gene            15593..16183
                     /locus_tag="PKM94_07385"
     CDS             15593..16183
                     /locus_tag="PKM94_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66502.1"
                     /translation="MITTAPDSITKIPSLQGALINLRPVRPADIRAIARYANDPSVAR
                     WVPNLPHPYTSAHALQWVRLAKQLASDAAGYSFGIEHPDSGEIIGMIGLKDVDWTDRR
                     AEVGYWLAKTMWRRGYMEEALGLAARFAFRDLRLHRLTALVLAPNVGSARLLEKVGFR
                     YEGTRRKDCRLRRRWYDVAVYGLLAREWAGPPARRP"
     gene            16359..20459
                     /locus_tag="PKM94_07390"
     CDS             16359..20459
                     /locus_tag="PKM94_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HOD66503.1"
                     /translation="MKGRVRARLRRRHALLSALAAGLLLIPGLASAETAPPIGDIHRP
                     FSVRTTDAEARQLQAESAPGLHAALAGAAGPQRLEVPLPTGEAALLELERFDVVAPDA
                     RFVIAGPGGGADLPPSDVLLYRGRIAGDEYSSAFLAVSGSGMINGFIDAAGTRYLLAT
                     PSASLKSGDRTVTIREAAGIGELEVPFCGTEPGSAFDDDLEMVKEAALKTSAGPKLLY
                     LAIDADQAYVNMFGSAVEARDYIVQLAGAASAIYVRDLDLRLWLRFARLWPSGGEPFS
                     PQDLYGFRDYWQVNEDPSPFNIIHMFSGRRDTPYGGVSFISNTCNGNAYGIDALLNGS
                     FTAPVTYPDNGNWDINVFPHEMGHNCGTYHTHDDQYNPHIDDCGNGTPSVSTMMSYCH
                     TFQGYQRNIDLRFHRRVVGIIRSVTYAAGCHGFDCNGNGVPDDADIAAGSSLDVNSDG
                     IRDECQDCNGNGTLDPLEIAGGAPDVDGNGILDACEADCNSNGLPDRYETWALLAADA
                     DGNFRPDACDPDCDNNGQLDYSQIQANLALDLDRNRVLDQCQDCNGNFVPDWMDMGRQ
                     FNVFTGDRVLTGIAEFHEASGVTVGSYPSVATANDVLADPAGSYMWVAATDGQVYRHA
                     VGSGTLSVFIAAGSGGLGQPSGLLIPPSGELLVLDLTNAAVRRYNRSTGAFLGNLVAP
                     GGGGVTQPRGMVFSPDGASLLVNSSDNGVYRFLAATGASLGLFVSPGSGGLSGPRGMA
                     FLPNGNLVVSSYNTNQVLEYDGTTGAFVREFTDEFGLSLPWGLRIGPNGHLFIGAEGA
                     VFEYFPDLGKYYRRFVRGTSAVDQVSGLDFLPASPHDVNGNRVLDVCEAGDLDGDGVP
                     NVNDNCPTHPNPAQTDGDGDGAGDACDNCLATVNPDQRDVDNDTHGDLCDDCPAEYDP
                     AQADSDGDGRGDACDNCPALANADQADDDFDFVGNLCDLCPNDPINDPDGDDLCASVD
                     NCPLNYNPGQEDLNGNGVGDACELLVYDTVATQSTQLIIGSTGNFAREGTGGATLDYY
                     DAGDCEWIYLYDGSPVIAYQSGPATIAASSLYGDNGFKNDPAGKLSVPTIDSGAFQVF
                     RSANVTTADGTIGLQKIWYAPRQPDTAQFVIQRLRVYSADGAVHTGVAIGEFMDWDIP
                     GSGNAGGIDAVRNLIYLQGSGSGCEDNSLRQGGVALLGTVVDSLCIDPAAVPYGAITQ
                     ANSVYIYPTGNLVPSEVWTLMQQPGYSALTSYTDQFILMTYYNAASVAPGDTIDIYSV
                     LATTLGSAPRTLPVVVDQARVWFAGHVGCYQGGGCCVLRGDFNDDGSVKVSDLTGLVN
                     YLFRGGPAPACLDHGDTNGDGSIKVSDLTLLINYLFRGGPPPVACP"
     gene            20703..22988
                     /locus_tag="PKM94_07395"
     CDS             20703..22988
                     /locus_tag="PKM94_07395"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOD66504.1"
                     /translation="MCKSVASLVVAGVYLLFGSTVLSAPVSTVASPSFHVPPAFVSNH
                     GQWSPEVHFRAKVGDLTAWLTPGGVVYGLRGARELVRARFLDARPAPVMSPSEPAGFA
                     VNFLYGSRPDAWVADAGAFGRVTLEQIYPGIDAAWYLRDGRLEYDLILAGGADPGTIR
                     MAYEGADTLAICDSGDLLVMVGQDTLRESAPVVWANAGGSRHRLDCRFVLLGPTTVGF
                     SVEAGPAPAFGLVIDPILKYSTYLGGSEADLATDLAVEPDGSICVVGQTSSANFPTLG
                     AMDPTYNSGLDIFVARFAANGGALLTATFIGGSLDDEATSVSIDLDGNIYVTGATQST
                     DFPVKAAYDSVQNGEADVFLVKLAAGGGSIVFATYLGGLENEKAEAVAVGPNGLIWLT
                     GRTESPGFPKVNAIDATLNGAYDAFVSLFPPAGKPLSFSTYLGGNFADEGRDAAPVSY
                     GVYISGLTESPLFPVTAGAYDVSYNGNGDMWVAKLAFSGGLGSLSYSTFLGGTGPETD
                     DCSINLMADGRVAVAGMSGSADFPVSPDAFDPSYNDNGDATISVLSPDLADLDYSTFF
                     GGSSYEGAVGTAADADGNIFIVGPTFSTNLPLLHPFDSANMNIDGFVARLNERDGLVF
                     STYYGGSQIDLIAAAVVDQAGSVVFAGQTSSTDLPVGNAWDPTYNAGGSDGFVAKLYG
                     INSGCCQERRGNINDDDVETPNISDLIFLVNYLFRAGPTPPCLLEANVDGDPGGQLNV
                     ADITRMVNFMFRGGDPLPLCP"
     gene            23391..24827
                     /locus_tag="PKM94_07400"
     CDS             23391..24827
                     /locus_tag="PKM94_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD66505.1"
                     /translation="MRYPLMLLYLLGLGGSVCSQGLDSVWSRVYDFRQTPDDWYVLND
                     GVHTSDNSFVLIGCVQPVGPEGQLFIVKIGKDGDTAWSKTVGDELTHDVGVGIIEMSS
                     SDLMAMSIVQFEGNWGRIRLHKMTPGGDVIWERTYLAETEYAYGYGIAETADSGTVVT
                     GVSESDMFVLKTDSNGDSLWIRYFGGDFYDAGVAVAPTSDGGAIAVGYLSTADQKYDS
                     LVVVRVDPLGDEVWGKVFPFSDGEVFDASADIYRDEGGEYILTATPDFGPVFSYKVDE
                     NGTVLWSSDLDQDGYLTVLDICEGRNESTYITGSRGPAPHTLVGKIDSDGDSLFLSSL
                     VLSENSTGEFIHQDDSGYIYVGGRLLQSHTFYIAKLKEDTESDIAEDRAITAPIYALS
                     QNYPNPFNPSTRIEFSLPASTHVNMDIFNILGQRVRTLVDEDRPAGVYAVVWDGIDQD
                     GGQVASGLYFCRLIAGDFVQSKKMLLTR"
     gene            25639..>26812
                     /locus_tag="PKM94_07405"
     CDS             25639..>26812
                     /locus_tag="PKM94_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOD66506.1"
                     /translation="MTEAIRRYFRFEEHGATFRRELIGGATTFMTMSYIIIVNPKILE
                     VAGIPFGPSMVATILTAFFGTLLMGVYARRPFAIAPYMGENAFIAFTVVQVLGYSWQS
                     ALGAIFIGGLLFTLITIFGVRSWLANAIPQNLKIGFAVGIGLFLTFIGLNETGIVGLG
                     VEGAPVHVGDFGTTSILLAIVGFVLIAVMMLRRVKAALLMGILLTTVLAFLFGEIRWP
                     ADWVSLPPSLGPIFLKLDIGAALTWGFFSVILTVLVMDFVDTMGTLLGLSLKAGLLDD
                     RGNLPEMEKPMLCDALATVFAALVGTSTAGAFIESATGIQSGARTGLASVFTALLFLL
                     ALFLAPFLTVVPPAAYGPALIIVGLLMLSPITKLDFDDLSEAVPAFVVIALMSFTYN"
BASE COUNT         4725 a         8725 c         8536 g         4826 t
ORIGIN      
        1 agggggcgct caagctcaag gaaatctcct acatccacgc cgagggctac ccggcggccg
       61 agatgaaaca cgggccgatc gcgctcatcg atgaaaacat gccggtcgtg gtgatcgccc
      121 tgaaagatcc ggtgcacgac aaggtgatga gcaacatcgc cgagatccgc gcccgcaacg
      181 gccaggtgat cgccatcgcc accgaggggg acaccgagat cgccgaccgc gtcaatcacg
      241 tcatctatgt cccgccgacc taccggctgt tcacgccgct cttgtcggtg attccgctgc
      301 aactgctggc ctatcacatc gccgtgctgc gcggctgccc ggtcgatcag ccgcggaatc
      361 tcgccaagag cgtgacggtc gagtagaccg tttcgcacat gagtgtcgac ggcggcccgc
      421 accggcggac cgccgtttct gttggcgggg aaggggggct gcgatgggga gtcaccttga
      481 acgctttctc aactggtcat aacggccccc cggtctcgcc gggcgggtcc cacttcggtg
      541 ctgtagacct gaccgttgag tctgattcgg cgagagccgg acctctgctt ggtgtcgggg
      601 gtcctaccga gtcaaggccc cgatgtcgga aagtactgac gggcgaacgg cgacacggcg
      661 gccccgaccc cactctccgt aaacgttggt gaccgtcagc tttcggatcc gattacacta
      721 tggcagaacc aacgacactc gctggaaagt aatcacttgg ggttcgccga ccgaacctgc
      781 ctggactcaa tcctgagcga cgtggttcag tgctgatgcc gatggtgcaa gtccggccaa
      841 tgcggaagcc ggtgcaccac aggtttgtgc aggtgcttat gcgtgtgccg acccgatatg
      901 atgctctcat gcacgtgttc atgatgtccg tcgtcgtgac tgtggctgtg ctcgtggtcc
      961 agtgcctcgt gctcgtgttc gtgggcgtgc tgctcccgaa atagcagcaa taacgacaga
     1021 gccagaagca atccggcagc aacatgaacg acagttattt catcattgag catgatgacc
     1081 gagagaatca cgccgaagaa cggatttgcc gcgaagaaca tttggccacg cgttgctccg
     1141 atgttctgcg cggatgtgat gtaaaggaca atgcttgcgc cgtaacagac gattcctacg
     1201 ccgagcgcgg cggcagtcgt agccgcagtc gcggcgtatt cgctcgacac gagcccaatg
     1261 ccgaggttga ccgtaccagc cacgatcccc ttccaaaacg tgctctgggc aggcgtaata
     1321 ccatcgatga gggaagtgaa attgttgtcg aatccccaac cgagacaagc tcccatgatc
     1381 agcaacccgg cgactactcc ggcgctcttt tcgccaatag acaatagtgt tccggcaagc
     1441 agcacaccaa ccatgccaaa ccacccaaac cgtcccagat gatcgcgaaa caggagagta
     1501 ccaaggacag cggttgccac aagttcgagc ggtaaccaaa gcgacaccga cgccgctgac
     1561 gcagctttaa gtccaaacaa taggagaact ggagccagca cacccccaaa gaacacagcg
     1621 ccggccaatc ggaattggtt tctccggtcc attcgccatg gcagcgtgta gggccgcttt
     1681 ccgaaagcca gccccgatgc tccaatggcg gcgcccaggt acagtaaccc agccagctga
     1741 aaggcatcaa gagttgagag caacgccttg ctgaacggag tcgccgctcc aaacagagcg
     1801 gccgaaagaa gggcaagaaa cacgtaggac atcctgcaat aagatgcctt ctttcgaatg
     1861 ctgtttcaag aaagtcctga tcatgcttcg gccggctcca tgattgtcgc aattcaacac
     1921 tcccgattag aaggggatga gcgctgtatc gaataatgca tcgaaggccg gaaggagatt
     1981 gagatgaaat ggataacccg ttccaaagtc aaagttgacc gcgtcgcctg tccgtggctc
     2041 atcaacaggt tcgtcgacag gaatgccgag ttcatcttcg tcccggcaaa tcaggcagtg
     2101 gcggaagcga agaaacagaa tgccattgcg ttcgacatcc ctggcgtcga accaggccat
     2161 catggccggg aatgttcatt tgaagcgatc ctcaaaaagt acagactcac agacgatcct
     2221 gccctcacgc tactcggtaa gattgtcaat ggtgcggaca cagataatca attgtggaat
     2281 cagccggagg gacccgggtt gagtgcaatc gccgaaggat tccgccatct cggattcgag
     2341 gatgatcgcg aaatcaacac tgctgagtgg atagtctacg atgctatata tgcttgttgt
     2401 caggagatga tcaggcaggg caggcacgac ggtgctttga aggaatgaac ctgaggccgg
     2461 aacccacagc cgagccgccg tcgtatgctc tcgaacgaca acaacttaca gatgcggcgt
     2521 acttcaacag gccggcccgc cggaggactt caccgggtgt gcatcccccc tactgctccg
     2581 cctccggcag atcccgctcg agctgtttgt cggaccggaa accgagggcg tagtcggcct
     2641 gcatgagggt gtggatctcg cgcgtgccct cgtaaatgga cgcccctttc gcgttccggt
     2701 agaaccgttc caccgggtac tcatcggaga agccgtacgc gccgaagacc tgcacggcgt
     2761 tcgccgccgc cgcctccgcc tcgcggcagg cgatccactt ggccagcgag gtcgctttgg
     2821 tcgagcgtac cccccggttt ttcagccagc ccgctttcat ccagagatag gtgcagtact
     2881 cgtagccggc ctccatgttg gcgatcatct gcttgaccag ctggtgctcg ccgatgggct
     2941 gctcgaacgt gcgccggtcg agggcgtagg cgacgctcgc atcgcggcag gcgcgtatca
     3001 gcccgcacga tccggccgcc accgtgtagc gcccctgatc gagacagaac atcgcgatct
     3061 tgaacccctg cccctctccg tacaccaggt tctcggcggg gactctgaca tccgcgaacg
     3121 cgaggtaccc ggtgttgccg gcgcggaccc cgagcttgcc gtggagcgag ccggtcgaaa
     3181 cgccctccca gccgcgttcg acgatgaacg ccgagaggcc ggaatggtcc cgcgcgcgct
     3241 ttttgtccag atccgtccag gcgaacacga ggaactggtc ggccacgtcg gccagcgata
     3301 tccacatctt ctcgccgttc aggacatagt ggtcgccgcg cctgaccgcc gtcgactgga
     3361 tccccaccgc atccgacccg gcagccggtt cggtcaggcc gaaagtcgcg atcttctccc
     3421 cccgggccgc cggaactagg tacttccgct tctgcgcctc gttggcccag gtcagaacgg
     3481 gcagcgagaa caggccgatg tgaaccgaga ggatgacgcg cgccgaggtg tcggcgtact
     3541 cgagctcctc cgaggccagg ccgagagaga tgtagtcggt tcccagcccg ccgtactcct
     3601 cggggatgca gaagcccagg aggttggcct ctcgcatggc ggggagcacc tcggggttca
     3661 tctgctgcct gcggtcgtac tcggtgatgg tgggggcgac aacgcggcgg gcgagttcgc
     3721 gggccatgtc gcgcacagcc aactgcttgt cggtcaggct gaaatcgatc atcgggccgg
     3781 gttctccttg aagttggtgg ttccgctccg gatatatacg cagtccgggt tcccctgtca
     3841 aaccgggggg gcgggcggtt tgtgcccgga atcgcggcgc tctttggcgt ttggatcgga
     3901 caaaatgggt cggatcagcc cgcatcgacc gcccgttgcc ggacccgccc ttttcgcgta
     3961 tattgccgca ccctctgccg ggggaaaacg aagcgcgaag gaccagcagt gacggaacca
     4021 gcacgtgtca aggccgacct tgtccccttc cgacccgagg acgcggaaaa ggtcatgtcg
     4081 tggatcgact ccggggagac gctgcagtac ctgtgcggtg acgacacctg gccgccggcc
     4141 gaggatctgt tggcgtcgtg gcagaaagcc gacgcggcct cgtacctcct gctggccgac
     4201 cgccggccgg tcgcctacgc ggagatctgg gaccgtcccc tcgagcaggc ggccgagatc
     4261 gggcacctgc tggtcgaccc ggcgcggcgt ggccgcggct acggcactct catgctgcgg
     4321 ctgctcgtcg agcgggccgc acaccatccc cgcgtgcgcc gggtggtgct gaattttcac
     4381 cacggcgacg aaacggtgct cggctgttac ctgaaagccg ggtttgagct ggtgggcagc
     4441 ggaccggacg gcgaggggct gcgcatggag cgcccggcgg ggtagcgcgg aggcgcgcca
     4501 ccgccaggcg cggcgcccgg aaaacgtgga gatgacgaca agcggactcc gtacgatact
     4561 cttaacgata gcgtaaggac tttctgtatg accgagtaca agaacatcat cgtcaggaaa
     4621 gacgcgggct gtctctgggt gaccatcaac cgggagcggg cgctcaacgc gctcaaccgc
     4681 gagactatcg atgaactgca gcagctgttc agtttctatt ggaccgacga cgaggtgcgg
     4741 gtggtcgtgg tcaccggaac gggtgagaaa gcatttgtcg ccggcgccga tatcaccgag
     4801 ctcgctgaaa ccgaccttcg cacgggcacc gactactcgg cccggggcct gtacttgatg
     4861 aagacgatcc agaattttcc caggccggtg atcgccatgg tcaacgggtt cgcgctcggc
     4921 ggcgggtgcg agctggccat ggcctgcgac attcggatcg cctccgacaa ggccaagttc
     4981 ggccagcccg aggtcaacct cggcatcatc cccggattcg gcggcaccca gcggctcccc
     5041 cgactggtcg ggcgcggcac ggcgctgaag atgatcctca ccggagagat gatcgcggcc
     5101 gacgaggcca agcgaatcgg gctcgtccag gaggtggttc cggcggccga attgaccgcc
     5161 cgcgtgcagg caatcgtcga gttgatctgc gcgaaagcgc cgatggcgat cgcggccgcg
     5221 aaagagtgcg tcaaccgcgg gctcgatgtc tccatgtcgg tcgggtgcga cctggagaaa
     5281 gtgagtttcg gccagatgtg cgcgaccggg gacaagaacg aggggtgcga ggccttcctg
     5341 gagaaacgca agccgatgtt cacgggacac tgaggggcgc gttcgaattg ccgccggcgc
     5401 cgggccgtgc acccggcgcc gcgcgatgcc gcgacccggc ggcgctgctg cgccgcaccc
     5461 ctctggatcc tctcccgcgc aggggtgctc tcctccgtgt gcaaagccgc cgtcgcgacg
     5521 acaaccgcca tcaacgaagt caggagtcat tgcgccatcc tgcttccctg cggaaacggg
     5581 gtctgttaca ttgcggggaa accggaggct gcgaacttgt caagggagca ctaatgcaga
     5641 gcgacgccat ccccctccat cggctctatg gcgacctggc ctggctctgg ccgctcatga
     5701 gtccgcccgc ggagtacgtc gccgaggccg cccactggaa ggccgccctt cgcgaaaacc
     5761 tcggccccgg ccgccgctgc ctgctcgagc tcggcgtcgg cggtgggcac aacctgtcgc
     5821 acctgaccgg cgaattcgat gccgtggcgg tcgacctctc cgagcctatg ctggcgcact
     5881 cgcgccgcct caacccgggg gtggagcact tccttggaga catgcgcacg gtgcggctgg
     5941 ggcggacgtt cgatgccgtg gtgatccacg atgcgattgc gtacatgctg agcggggacg
     6001 atctgcgcgc cgcgttcgcc accgccgccg cccatctggg caccggcggg gtgttcgtca
     6061 cctccccgga ctacgtggcc gagacgttcg tcgacgggtc gatcgcctgc gagacgcggc
     6121 cgttcgacgg cgggtcgttc acctatgtcg agtaccaatg ggatcccgac ccgtccgaca
     6181 cgcagaccga gacggtgttc acgtatatta ttcgccgcgg cgggaaggtg cagattgagc
     6241 aggaccggca cctgttcggg ctgtttccgc tccgaacctg ggaatcgctg atggcgcagg
     6301 ccgggttcgc ggtcgcgcgc cgcgactacc cggtgcgtga cgatggccag ccgggatggc
     6361 tgttgatcgg gagaaagctc tagtccagcg aggagcgcat ggggataaca atcaatccgg
     6421 tggccgaaag cgaccgcccc tgggtggagg aggttttccg ctcctggggg gccgattttg
     6481 tcgtcacccg cgggcgcaag gtctgcccgg aggaaaccga ggggctgtat gcggccgacg
     6541 agcgggggag gcggatcggt ctgctcactt tccagatcgc cgggacgcag tgcgaggtcg
     6601 tgacgctcga cgcgttcgag aagttccggg gcatcggcac ggccctgatg gaccgcgcgg
     6661 tcgctctcgc ccgcgcgcgt gggtgcaccc gcgtctggtt gatcacgacc aacgacaacc
     6721 tcgatgcgat tcgcttctac caccggcggg ggttggtgat cgcccatgtg catgtcggag
     6781 ccatcacgga gtcgcgtcgg ctcaaaccga gcatcccgct cctcggcgcc tacgggattc
     6841 cgatccggga cgagatcgag ttcgagctgg ttctctgacc cggccagggg ctctttacag
     6901 cggcccgccg ggtgcgtata ctacggacgc cggttgcccg cgggatcgcc ggcggacgga
     6961 cggtgcggcg gcgggaatcc ggccgcatct cgattgcgaa ggaggactcc atgaaaacgt
     7021 tctttctgtt caccaagctc gcgccgtcgg acggcaagcg gatgcgcgag cggcagtcgc
     7081 tcgggcgggc gtggctccac gaggtcaagg agaagtgccc ggacgtgaaa ttcctctccc
     7141 actacgcgct cctcggcgac tatgatttcg tcgacatcta cgaggcgccc gacgaagaga
     7201 cggcgatgaa ggtctcgatg atcagcatgg acaagggggc gttccaggcc aagagcgtcc
     7261 tggcgatccc ctacgcccgc ctggtggaac tggccaatga gttgtgaggc ggagggggag
     7321 aaccccgtcg ccgggccgag cgccgcgacc gggtgcgtcg gagcatcggg aaatcgactt
     7381 gcgggcgcgg acgaaagcat tctccggccg ggactgcggc ggtcccggcg gtcgtgaatg
     7441 gtgacctgtg agtaaaccac cgatgggaca atccgctctc acgtttcacc cgctcactcc
     7501 ggagcgatgg ggtgattttg aggccctctt cggcgtgcgt ggcgcctgcg gcggatgctg
     7561 gtgcatgtac tggcggctgt tccgcctcgc attctcgatc ggcaaggggg agggcaaccg
     7621 ccgggccatg cagaaactcg tccgggcggg aacggaaccg ggcattctcg cctacgacgg
     7681 cgagcggccg gtcggctggt gcgcggtggc gccccgggag gagtatgtcc ggctaacggc
     7741 ggcgcgcacg ctcaagccga tcgacaccca gccggtctgg tcgatcgtgt gcctcttcgt
     7801 ggacaagggc taccggcggc ggaacgtgtc gaccgagctg atccgcgccg ccgcggcgtt
     7861 cgcttgcgcg cggggcgccc gtatcgtcga ggggtatccc tacgactacc gtggccgcgc
     7921 gaagcaccag ccgccgccgt ttgtgtacac cgggctgatt caggctttcg agcgggcggg
     7981 gtttgtcgag gtcgcgcggc cgtcgaaatc acgcgccatc gtccgcctca cccggcgctg
     8041 acccaagccg cctactccat caccagccgc agcggcctca ggtggccggc atgcttcttc
     8101 tcggcgttgt cgaagacggc gatgccgaga tagagttcgc tctgaaaacc gatatcggtc
     8161 ggcgatccgg tcgccagcag gcgcgacagc tcgaccgtcc acacgccctc gcgccacacg
     8221 ccccgcgcct cgatgtcgcc ccggtcgccg gttgacggcg ccacgagcac cgccggaatg
     8281 cggtgcccga gcggaaaggt gtcggcccga tggtcgtagg cctcgtaccc cggtgtcccg
     8341 tagagcagcc agtcgcggaa gaggcggtgg gtggggacga agtaggggaa gcggaggatg
     8401 ctgtcgaggt tgtcgcgata gccgccggcg gcgaggttgt cgaagaaccg cccgtcgccg
     8461 ctctccccgg ccggggccgc ccagtagcgg tcgtccgcct ctgccgtcgg gttggtgctc
     8521 accgccatcc acacccacac gtcggccgtg tcgccgccgg tgaaatgccg tccggggcgg
     8581 ctcgcgcggc cgacgtggca ggtcgaggca caccctcccg agcggctgaa atagagcgcc
     8641 gcctggtcct cgaagaagat gtcctcgccg tacgggccgg tgaaaaccga ccgctccggg
     8701 atccatccgg tgtccgtctt gacgaggtgg cggttgagtg agcgggtgtc gtcgggccag
     8761 cgcaggagga agaagatgcg ctggcggtcg cgcgcagtct tgaccgtcac cggcgcagcc
     8821 gcatagagga agttggcgcc gccctgggtt tggacgatgg ccgaatctgc accggcccac
     8881 tcgccctccc ccaggtaacc gtcgatcacg atcgggctgc ccgcagcccg gcaggcgagc
     8941 gtcggcggcc ggccgaggga gacgtggagc gcggtcccgc cggccacggc gacggcggcg
     9001 gcgattgcca gcgcgggcca cctcaccgcg ccgaccaagg gcttcccgga aaacatgcag
     9061 gcgacccgcc cgcgcagcga cagagaaacc tccagcgtgt gcagcgccgc gaagaggacg
     9121 aggagccgga agctccactg gtgcacgaag ctgttgaaca ggtagccggg gccgccggag
     9181 tagagaccgg ccgcgaggct tgtgccggtg aggagtgcgg cggcggcgag gaccagcatg
     9241 gacaggtatg tcagcttccg cgtgaacgaa tattcggacc tgtggaagag atcggtcagc
     9301 cggcggtgct cgccggtgaa caggagatag gcgcaataga agatgccggt ggcggccaag
     9361 agcacgccgg cgaaaaggtg gaggcggtag atgttgccgg tcggggtaag ccggtcgatg
     9421 agcgcgcgga cctcgggtga cagccactcc gcgccgttga cgacgacgat gcgcaagccg
     9481 gtcgccgcca agaggatgaa cagcgcgatg gccagcgcgt gggcggtccg tgcgggtaag
     9541 ctgatcgtgg gggccgggcg cgacatgctt tcataatgcg gagggtggcc gcgccggcca
     9601 agcgatttgt tgcccgcggg cgaatttccc ttggataatc gccgcgccgg cccgtacatt
     9661 atagcatgca gggtgcggtg tgaaccgcgt gtcgccgcgt tcactgacag gcgctctcag
     9721 gcgagtgcga ccccacgttg cgaaatgcac ggaccggcct gtgatcacgc ggttcggcct
     9781 cactactacc agtcaggagg ccgagatgtt tgcgaccgat tcagcgacca cccgccggcg
     9841 tatcgaggat ttgcgccatc aactcctcga actccggcgc caggtgcgcg agttgcggaa
     9901 aaccgtgccg gaggaggtga ttggccgcga gtacgtcttt gcgggtccgc gcggaatccg
     9961 catcaccctc gccgacctct tcggggaccg ggacgagctc atcatcatac acaacatggg
    10021 acgcgcctgc cggtactgca cgctctgggc ggacggcctc aacggtctcg tcccgcacct
    10081 ggccggccgg gcgtctctcg tcctcgtgtc gccggatccg cccgaaatcc agcaggcgtt
    10141 cgccgcctcc cgcgggtggc agttcgacct ctggtcgacc ggcgccgagt tcctcgtcga
    10201 catgaagatg gccgacgaga agggttcccc ctggccgggg atctccgcct tcgtccggcg
    10261 gcccgacggg accatcgtgc gcaccgcttt cgattacttc ggcccgggcg acgactactg
    10321 cgcggtctgg cacatctggg acatgttccc ttccggcgcc gggggatggg agccgcgcga
    10381 gggctgaacg ggagggccgc cccgccaacg cgccgtgcgc agccgcggcc gggagccatg
    10441 aattcggcgc ggaccgcaac aatcctcttg tgccggcgta tctaatagac taagttggtc
    10501 tattatcctg ccggcggccg gcaggggcag agaggagacc gcgaccacgg tggcggacac
    10561 gctgcgactc atgggcatcc tggcgcaccc ggacgacgaa tcgctcggca tgggcggagc
    10621 cttcgcccgc tacgcccgcg agggggttga aacctttctc atcaccgcca cccggggcga
    10681 gcgcggccgg tttcgtgatc tccgcggcga ggcccgcccg ccgatgccgg tcgtggcccg
    10741 cgcgcgcgag ggagagctcc gcgcggcggc ccgcgttctg ggcgtccggg aggtccactt
    10801 cctggactat gtcgacggcg acctcgaccg ggccgaaccg gccgaggcga tcgcccggat
    10861 cgtcggccac ctgcggcggc tgaacccgca ggtggtggtg acgttcgcgc ccgacggcgc
    10921 ctacggccac ccggaccaca ttgcgatttc gcagttcacc accgccgcca tcgtcgccgc
    10981 cgccgatccg gccttccccg ctcccggcgc gcctccccac gcagtcgcca agctgtacta
    11041 cctggcctgg accagggcca agtgggatgc gtatcaggcg gcgttcaagc agctcgcgac
    11101 gacggtcgac ggggttgagc gccgggcgac tccctggccc gactgggcga tcaccacggt
    11161 cgtcgacacc gccgcctact gggcggtcgc gtgggaggcc atctcctgcc acgagaccca
    11221 gctggcgatc tacaccaagc tggccgacct gccccccgag taccaccgct ccctctgggg
    11281 cacgcaggaa ttctaccgcg ctttcagcct cgttaacggc ggccggaagc gcgagaccga
    11341 cttgttcgag ggactgcgct gaccgacacc cgaaacgtcc gctacctggg agagacccgt
    11401 gaacagcaat gaccgcactg ccgcccgcca tacgccgatc gatctgtcgc cggaggaatt
    11461 ccgggcgctc ggccgcgacc tcgtggaccg ccttgccggg ctgttcgcct cgctgcgcac
    11521 ccgtccggtg acgcccgagg cggcgccggg ccgcatccgc gagcgcctcg gcgccgaccg
    11581 cccgctgccc gatcagggat ccgacccggc cgcgctgctg gccgacgccg ccgacctgtt
    11641 gtttgagcac tctctgttca acggccaccc ccgcttctgg ggatacatca cctcctcgcc
    11701 cgcgccgatc ggcgcgctcg ccgacctgct cgcggcggcg gtgaatccga atgtcggcgg
    11761 gtggaaactg tcgccgatgg cctccgagat cgaggcccag accgtccgct ggatcgccga
    11821 actggtcggg taccccgccg actgcggcgg cctgctcacc agcggcggca acgccgccaa
    11881 cttcatcggc atcctcgccg cccgcgccgc gtgcgcgccc tgggccgtgc gcgaggaggg
    11941 gatgcgcggg ggccggcgca tgatcatcta cgcctcctcc gagacgcaca cctggctgca
    12001 gaaggccgcc gacctgttcg gattcggcac cgaggcgatc gcctggatcc cggtcgaccg
    12061 tttccggcgg atggatctcg cccgccttga ggcgcgggtg cggaccgacc gggccgaggg
    12121 acactttccg ctggcggtcg tgggctcggc ggggacggtg agcaccgggg cggtcgaccc
    12181 gctccgcgac ctcgctgcgt tcggccgcga ccaggggctg tggttccatg tcgacggcgc
    12241 ctacggcggg ttcgcggccg cgctcccgga agcgtccgag gatctgcaca gcctcgccct
    12301 ggccgattcg ctcgcgatcg atccgcacaa gtggctctat gcgccgctgg aggccggctg
    12361 cgcgctggtg agggatgccg agaagctgcg ccacgccttc tcctaccacc cgccgtacta
    12421 ccatttcgag gaggaagcga tcaactactt cgactacggc ctgcagaatt cccgcggttt
    12481 ccgcgcgctc aaagtctggc tggccctccg ccaggtcggt cgggcgggct atgtgcagat
    12541 gatccgcgac gacatcgccc tcgcccgcca cctcttcgcg catctcgccg cgtatccgga
    12601 cctggagcgt ttcacctgcg atcttagcat cgccactttt cgctacgtgc cggcggacct
    12661 gcgggccggc ggcggggagg aggcccggga gtacctgaat gaactgaacg aaaatatcct
    12721 tgagcgcatt gagcgcgggg gagaggcctt cctctcgccg gcgatgatcg agggcgtcta
    12781 tgcgctgcgc gcctgcgtgg tgaattttcg caccacgcgc gaggacatcg aggcgctccc
    12841 ggaaatcgtc gtgcgggtcg ggcgcgcggc ggacgcggcg cggcgcgaaa agacactccg
    12901 ccggtagcgg gccgccggcg ctccctcagg taagccggtg gcgcatccag acattcggct
    12961 caaggtactc ggcccggtcc cgctcgcggt ccacccggac cggcgccagc ccgccgggga
    13021 caacgtactc gccggaaccg gggaaggtca gccccgtcca cgcttcccac tgggcgatcg
    13081 agccggggat gtacatcgag cgcgcgcaga cgcccacgat ctccgcgccc aggcgggcgt
    13141 gcacacgcat ccacggatcg aacggcagtc cgtcatcgcg cgtccaccgg atgtaatgct
    13201 ccatggggat cagcgggtag tcgcctttgc ggttgggccg caccggggcg aacaggttac
    13261 ggcagccgcg ggcccgcccg atcgccgcca tggccgccac cacctgtccg ctcaggccat
    13321 gcccgagctg cgcacggtcg accatgatct gcagggcgaa ctggttcttt ggtgcaaacg
    13381 tcgcccgcgc cgccagctcc gtgagtatcc agtcgatccc ggtgtccgga agctcggccg
    13441 gttttccctc ccatggcagg gggcaggaat tgccgaccgc gatcacccgc cgatcttcgc
    13501 cgtcgagcag gaagagctgg tattccgggt agcgttcgta gagcaggcgc cagttgtcct
    13561 cgacgaccgg atcccggagc atgaatttcg gccacaagtc cgatgccagc cggttgattt
    13621 ccgattccaa gccgggacag tcgcggagcg tggccacggt gaacggcgga tgagacatgc
    13681 aatgcttcct ctacacggct ttggggccga actttgccgg ccagtatagc acgcgcgcgg
    13741 gaaaaaggcc agccgaatca ggccggcgca attgacaact tgccccgccg cgacaaacgg
    13801 cgtatactga accgcacctg caaccaaggg gtcgctatga aaccggtcac ctgccgggcc
    13861 gaattggtcc aggatgacgc caccagcggc tgcggaatca cgctgccgtt cgacccgaaa
    13921 gcgcaattcg gcaaagttcg ggcgcccgtg gtcgccacga tcaaccggta ttcattccgc
    13981 acgaccgttt tctccatggg cggctgctac tggattccgg ttaataaatc caaccgtgac
    14041 gcggccggca ttgcgccggg agaccggttc aagctccgaa tcgcactcga tactgaaccc
    14101 cgcgccgtta ctccgccggc ggacttgaag gaagccctgg ccggcgatcc cacggccaaa
    14161 accgtttggg acaaactcag ctatacccac cgccgcgagt acgtcgagtg gatcgcgcag
    14221 gccaaaaagc cggagacccg ggcgcgccgc ctgcagaaaa cgctcgaaag gctgaaagaa
    14281 cctcggtgac ggcgctggcg gcagagcgct cgcccgatcc gcccgggagc aaaggtggct
    14341 tcgcggcggt tgtactcatt gatcgccgaa taaggagctt tcttcaataa ggagttttca
    14401 ccatggcggt atcaagcgcg gtgacagtcg gcaggaaagc cccggccttc gccctgccca
    14461 accagcaggg cgatacagct cggctctcgg attacgaggg gaaatgggtg gtcctctact
    14521 tctaccccaa ggacgatacg ccgggatgta cgaccgaagc ctgcgacttc accgataatc
    14581 tgagccggtt ggacaagctc aatgccgtcg tgctcggcgt ctcgcccgat tcggtcgaga
    14641 gccatgcgcg gttcgtggag aaacacaacc tgaaggtcac cctcctttcg gatcccgaga
    14701 agaaggtgct gcagatgtac ggcgccttcg gcgagaagat gatgtacggc aaggcgacgg
    14761 tcggggtgat tcgctccacc tatctgatcg atccgggcgg ccgcatcgcc tacgcttggc
    14821 ctaacgtgaa agcgaccggc catgctgaga aagtcgccgc gaaactggcg gagctggcgc
    14881 agcgatgaaa aagggacgca cccccgtgcc gccggtggcg ttgctactga caatcgagac
    14941 caaccggctt gcttcgacgt cccggccgga gttgcaaggt gaaaccacat gcgcctgacg
    15001 gacatacgat gatcgcccgt cgttctgcag ttccccgcgt gttgcccact ctcgatgggg
    15061 agcgggtgcg ccttcggcct ctgcgccatg atgacgcctt ggccatatgc cggtggctcc
    15121 aggatcctga gctatcccgc cgtctcgaac tgcttgagcg accgttcaca ttggggaccg
    15181 cccggatgtg ggttcggatc gccgtcgcag gtgtcgggga ccggaccggc ttctttttcg
    15241 cgatcgccga ttgcagaacc gacgatttgc tcgggatgat agctctgcat ggggtgaact
    15301 ggcatgaccg caacggcgag atcttctact ggctggcacg gccgttctgg cggagcggtc
    15361 tcaccgaaga tgcgctccgc ctgctcctcg gatttgcttt tcgccggctc cgccttcacc
    15421 gcgtctgggc cagcgtcgtc gaaaccaacc gcccttccac ggccctgctc gaaaaagccg
    15481 gcttctcccg cgaggtggta cggcgcgacg cgcgccgccg ggggcgcgcc tggttggata
    15541 tgtacaccta tggtctcctg ggggaggaat ttgccccgca gcgccgccgc gtatgataac
    15601 aaccgccccc gacagtatca ctaagatccc ctcccttcag ggcgctctca tcaacctccg
    15661 gccggtacgc ccggccgata tccgcgccat cgcccgctat gccaacgacc cctcggttgc
    15721 ccggtgggtg ccgaaccttc cccaccccta tacctccgcc cacgccctgc agtgggtgcg
    15781 gttggcgaag cagctggcga gcgatgccgc cgggtacagc tttggtatcg agcatcccga
    15841 ctcgggggaa attatcggta tgatcgggct gaaagatgtg gattggaccg accgccgggc
    15901 agaagtcggc tactggcttg cgaagaccat gtggcggcgc ggctatatgg aggaggcgct
    15961 tgggctggcc gcccggttcg ccttccgcga cctccgcctc caccgcctca ccgccctcgt
    16021 cctggccccc aacgtcggct ctgccagatt gctggagaaa gtcgggttcc ggtacgaggg
    16081 gacccggcgc aaggactgcc gcctccggcg ccgctggtac gatgtggccg tctacgggct
    16141 gctcgcgcgc gagtgggcgg gtccgccggc ccgccgcccg tgattgtcgt tgacggacgg
    16201 gggagatgag actatacttg tacatgtaga ggttcctatc gaccccgccc cggggccagg
    16261 ctcgcctccc cgaccgccgg gagtaccgtc tctagtccgt gtccgcgtcc atcgaatgaa
    16321 aagaataaag cgacgataag tatggaaagg atggtgccat gaaaggccgt gttcgcgccc
    16381 gactccgccg tcggcatgcg ttgctgtcgg cgctggccgc gggactgctc ctgatcccgg
    16441 gccttgcctc cgccgagacc gccccgccga tcggcgatat tcaccgcccc ttcagcgttc
    16501 gaacgaccga cgccgaggcg cggcagttgc aggccgagtc ggcgccgggt ctccacgccg
    16561 cgctggccgg agccgccggc cctcagcggc tcgaggttcc gctgccgacc ggcgaggcgg
    16621 cgctgctgga actggagcgc ttcgacgtcg tggcgccgga cgcccggttc gttatcgcgg
    16681 gccccggcgg cggcgccgac ctgcccccgt ccgacgtcct cctctaccgc ggccggatcg
    16741 ccggcgatga gtactcctcg gctttcctcg ccgtctccgg gagcggcatg atcaacggtt
    16801 tcatcgacgc ggcggggacg cgctatctgc tggcgacgcc gtccgcctcc ctgaaatcgg
    16861 gcgatcgcac ggtgaccatc cgcgaggccg ccggcatcgg cgaactcgaa gtgcccttct
    16921 gcggcacgga accggggagc gcgttcgacg acgatctgga gatggtcaag gaagcggcgc
    16981 tcaagacgtc ggccggaccg aaactgctgt atctggcgat tgacgccgac caggcctatg
    17041 tcaacatgtt cggcagcgcc gtggaggcgc gcgactacat cgtgcagctg gccggcgcgg
    17101 cgagcgccat ctacgtgcgc gacctcgacc tgcgcctgtg gctgcgtttc gcccgcctct
    17161 ggccgtccgg cggcgaaccc ttctcccccc aggatctcta cggattccgc gactactggc
    17221 aggtgaacga agacccctcg ccgttcaaca tcattcacat gttttccggg cggcgggaca
    17281 cgccctacgg cggcgtgtcc ttcatctcga atacctgcaa cggcaacgcc tacggaatcg
    17341 acgccctgct caacggcagc ttcacggccc ccgtcaccta ccccgacaac ggcaactggg
    17401 acatcaatgt attcccccac gaaatgggcc acaactgcgg cacgtaccac acgcacgacg
    17461 accagtacaa cccgcacatc gacgactgcg gcaacggcac gccgtcggtg agcaccatga
    17521 tgagctactg ccacactttc cagggatacc agcgcaatat cgacctgcgt tttcaccgcc
    17581 gggtggtcgg gatcatccgc tccgtcacct atgccgccgg ctgccacggc ttcgactgca
    17641 acggcaacgg ggttcccgac gacgccgaca tcgccgccgg gtcgagcctg gacgtcaaca
    17701 gcgacggtat tcgcgatgag tgccaggact gcaacggcaa cgggactctc gacccgctgg
    17761 agatcgccgg cggggctccc gatgtcgacg gcaacggcat tctcgacgcc tgcgaggccg
    17821 actgcaacag caacgggttg cccgaccggt acgagacgtg ggctctgctg gcggccgatg
    17881 ccgacgggaa tttccggccc gacgcctgcg accccgactg cgacaacaac ggccaactcg
    17941 attacagcca gatccaggcc aacctggctc tcgatctcga tcgcaaccgc gtgctcgacc
    18001 agtgccagga ctgcaatggg aacttcgtcc ccgactggat ggacatgggc cgccagttca
    18061 acgtgttcac gggcgaccgt gtgctcacgg ggatcgccga attccacgag gccagcggcg
    18121 tgaccgtggg cagctacccg tctgttgcca cggccaacga cgtgctggcc gacccggccg
    18181 gcagctacat gtgggtggcg gcgaccgacg gtcaggtgta tcggcacgcg gtcggcagcg
    18241 gcacgctcag cgtcttcatc gccgccggca gcggcggact cgggcagccc tccggcctgc
    18301 tcattccgcc gtccggcgaa ctgctcgtgc tcgacctgac caacgccgcc gtccgccgct
    18361 acaaccgcag caccggagcc ttcctcggca acctcgtcgc tcccggcggc ggcggggtga
    18421 cgcagccgcg cggcatggta ttcagccccg acggcgcttc cctgctggtc aacagctccg
    18481 acaacggcgt ctaccgcttc ctggccgcga ccggcgcctc tctcgggctg ttcgtttcgc
    18541 ccggcagcgg cggtctctcc ggcccgcgcg gcatggcctt cctccccaac ggcaacctgg
    18601 tcgtgtcgtc ctacaacacc aaccaggtgc ttgaatacga cggcacgacc ggcgcgtttg
    18661 tccgggagtt caccgatgag ttcggcctca gcttgccgtg ggggctccgc atcggcccga
    18721 acggccacct cttcatcggc gccgagggcg ccgtgttcga gtatttcccc gatctcggta
    18781 agtactaccg ccgctttgtg cgcggcacga gcgctgtcga ccaggtctcg ggtctcgact
    18841 tcctgcccgc ctcgccgcac gacgtcaacg gcaaccgcgt gctcgatgtc tgcgaggcgg
    18901 gcgaccttga cggcgacggc gtccccaatg tcaacgacaa ctgcccgaca catcccaatc
    18961 cggcgcagac cgatggcgac ggcgacggcg cgggcgacgc ctgcgacaac tgcctggcga
    19021 ccgtgaatcc cgaccagcgc gatgtggata acgacactca tggcgacctc tgcgacgatt
    19081 gtcccgccga gtacgatccc gcgcaggccg actccgacgg cgatgggcgc ggcgatgcct
    19141 gcgacaactg ccccgcgctg gccaacgccg accaggccga tgacgacttc gactttgtcg
    19201 gcaacctctg cgatctctgc ccaaacgacc cgatcaacga tcccgacggc gacgacctct
    19261 gcgcctcggt cgacaactgc ccgctcaact acaatcccgg gcaggaggat ctgaacggca
    19321 acggcgtcgg cgatgcctgc gaactgctgg tctacgacac cgtcgccacc cagtccacgc
    19381 agttgatcat cggaagcacg gggaacttcg cgcgcgaggg cacggggggc gccaccctcg
    19441 attactacga cgccggcgac tgcgagtgga tctacctcta cgacggatcg ccggtgatcg
    19501 cctaccagag cggcccggcc acgatcgccg cctcgtcgct ctacggcgac aacggtttca
    19561 agaacgaccc ggcgggcaag ctctcggtcc cgacgatcga ctccggcgcc ttccaggtct
    19621 tccgctcagc caacgtgacc acggccgacg ggacgatcgg cctgcagaaa atctggtatg
    19681 cgccgcggca gcccgacacc gcccagttcg tgatccagcg gctccgcgtg tattcggccg
    19741 acggggccgt ccacaccggc gtcgccatcg gcgagttcat ggactgggac atcccaggca
    19801 gcggcaacgc cggggggatc gatgctgtcc gcaacctgat ctacctgcag gggagcgggt
    19861 ccggctgcga ggacaactcc ctgcggcagg gcggagtcgc gctgctgggg acggtggtcg
    19921 acagtctctg catcgacccc gccgccgtgc cctacggagc gatcacccag gccaattccg
    19981 tctacatcta tccgaccggc aatcttgttc ccagcgaggt ctggacgctc atgcagcagc
    20041 ccggctactc cgctctgacg tcctacactg atcagttcat cctgatgacc tactacaacg
    20101 ccgcctcggt tgcgcccggc gacaccatcg acatctactc cgtcctggcg accacgctcg
    20161 ggtcggcgcc ccggaccctc ccggtggtgg tggaccaggc gcgggtgtgg ttcgccggcc
    20221 acgtcggctg ctaccagggc ggcggctgct gcgtacttcg cggggacttc aatgacgacg
    20281 gctccgtgaa ggtctccgac ctgaccggtc tggtcaacta cctgttccgg ggcggccccg
    20341 ccccggcctg cctcgaccac ggcgacacca acggcgacgg gtcgatcaag gtctcggatc
    20401 ttaccctgtt gatcaactac ctgttccggg gcggtccgcc cccggtcgcc tgcccgtagg
    20461 ggagaggcgg ccgcgcgagc tcgacggccc cccggttgcc ggggggccgt ttcttttcgc
    20521 cgcgggcacc gtccgacgca ctgtctgccc gtcccatgtg cgaatctcat tgacagaatc
    20581 ggaaaagccg atatattagg ggtaccctat tttgaggaat accgcagcat ctaatcgcta
    20641 accgatccgt agtccctggt cagtttaata ctaacgttct ttgccagtgg ggtagtgtac
    20701 ctatgtgcaa gtcggtggcc tccctcgtcg tagccggcgt gtatcttctc ttcggctcaa
    20761 ccgtcctttc cgcccccgtt tccacggtgg ccagcccctc gttccatgtc ccacctgcgt
    20821 tcgtttccaa ccatggccag tggagtccgg aggtccactt ccgggcaaag gtcggcgatc
    20881 tcaccgcctg gctgaccccg ggcggcgtcg tgtacggact gcgcggtgcc cgcgaactgg
    20941 tccgggcgcg gttcctcgat gcccggccgg cgccggtcat gtccccctcc gaaccggccg
    21001 ggtttgcggt caatttcctg tacggttccc gtcccgatgc ctgggtcgcc gatgccggcg
    21061 ctttcggccg ggtcaccctg gagcagattt accccggtat cgacgccgcc tggtacttgc
    21121 gggacgggcg cctggagtac gatctgatcc tcgccggcgg ggccgatccc gggacgatcc
    21181 ggatggccta cgagggcgcg gataccctgg cgatttgcga ttcgggcgat ctgctggtga
    21241 tggtcgggca ggatactctg cgcgagtccg ccccggtggt ttgggcgaac gccggcgggt
    21301 cccgccatcg cctcgactgc cggtttgtgc tcctgggtcc gacgacggtc ggattctccg
    21361 tggaggccgg tcccgccccg gctttcggcc tcgtgattga cccgatcctc aagtacagca
    21421 cgtaccttgg cgggagtgaa gccgacctcg cgaccgacct ggccgtcgag cctgacggca
    21481 gcatctgcgt ggtggggcag accagttcgg ccaacttccc cacgctcggg gccatggacc
    21541 cgacctataa ctcgggcctg gacatcttcg ttgcccggtt tgccgccaac ggcggcgcgc
    21601 tcctgacggc cacatttatt ggcggcagcc tcgatgacga ggcgaccagc gtgtcgatcg
    21661 atctcgacgg caacatttat gtgaccggcg ccacccagtc gactgatttc ccggtcaaag
    21721 ccgcctacga cagcgtgcag aacggcgagg ccgatgtctt tctcgtcaag ctggcggccg
    21781 gaggcggaag catcgttttc gccacctacc tcggcggact tgagaatgag aaagcggagg
    21841 cggtcgcggt cggcccgaac ggcctcatat ggctgaccgg ccggaccgaa tcccccggtt
    21901 ttcccaaagt caacgccatc gacgccaccc tcaacggcgc ttacgatgcg tttgtctcgc
    21961 tattcccgcc ggccggaaag ccgctgtcat tctcaaccta cctcggcggc aacttcgccg
    22021 atgagggccg cgatgcggct ccagtctcct acggggttta tatcagcggc ctgaccgaat
    22081 ccccgctgtt tccggtcacc gccggcgcct atgacgtgag ctacaacggc aacggcgaca
    22141 tgtgggtggc caaactggcc ttcagcggcg gtcttggctc cctcagctat tcgacctttc
    22201 tcggaggcac cggcccggag accgacgatt gctcgatcaa cctgatggcc gacgggcggg
    22261 tggcggtggc cggcatgtcc ggctcagccg atttccccgt gtcccccgat gccttcgacc
    22321 cctcgtacaa cgacaacggc gacgccacca tcagcgttct cagccccgat ctcgccgatc
    22381 tcgactacag caccttcttt ggaggttcct cctatgaagg cgcggtggga accgcggccg
    22441 atgccgacgg caacatcttt atcgtcgggc cgaccttctc gaccaatctc ccgctgctcc
    22501 acccgtttga cagcgcgaac atgaacatcg acggtttcgt ggcgcgactc aacgaaaggg
    22561 acggcctggt cttcagcacc tactacggcg gctcgcagat cgatctcatc gctgcggcgg
    22621 tggtcgatca agccggcagc gtcgtgttcg ccggccagac ctcctcgacc gacctccccg
    22681 tcggcaacgc ctgggatccg acctacaacg ccgggggatc cgatggtttc gtggccaaac
    22741 tctacggcat caattccggg tgctgtcagg agcggcgcgg aaacatcaac gatgatgacg
    22801 tcgaaacacc gaacatctcc gatctcattt tcctggtcaa ttatctcttc cgcgccggtc
    22861 ccacgccgcc gtgcctgctg gaggcgaatg tcgacggcga cccgggcggg cagttgaacg
    22921 tggccgatat cacgcgcatg gtcaatttca tgttccgcgg cggtgatccg cttcccctct
    22981 gtccctgagg cagccgcggg agtgtgctta aagcagcggt tgtgagttcg tccaacgccc
    23041 ggcgtccact ggcgacaccg ggcgttcttc cggcgaaccg cgcgatggcg ttttgtaggt
    23101 cagggtcttt atggtcctga cggcgccgtt tgcgtcgttg gcaagaatgt caggaccaca
    23161 agggtcctga cctacacaag atccagagga cgtccgttgc ttatgtgtcc ggctccaagt
    23221 gtcacctatc tgactagttc acccccgaga tgaacccacc ataaatggtg ggccacggtc
    23281 cgacaaacag agcgtcacta tcacgatagg gcgcggtcat ctatcttgtt gacaggcact
    23341 ttgtgagccg gttatgtgct gatgattgat ctaatactgg aggatctacc atgagatatc
    23401 ctcttatgtt gttgtatctt cttgggcttg gcggaagcgt gtgctcccag ggactagata
    23461 gtgtctggtc cagggtctac gatttcagac aaacccccga tgactggtat gtgctaaatg
    23521 atggtgtcca tacgtccgac aacagcttcg tcctaatcgg atgtgttcag cccgtcggac
    23581 ctgaagggca attgttcatt gtgaagatcg gcaaggatgg agatacagcc tggtccaaga
    23641 ccgtgggcga tgagttgact catgacgtcg gtgtcggcat catagaaatg agcagtagtg
    23701 atttgatggc tatgagtatt gtccagttcg agggcaattg gggccgcatc cggctccata
    23761 aaatgacgcc gggaggagat gtgatttggg aaaggacata tttggccgaa accgaatacg
    23821 cgtatgggta cggtatagct gaaacggccg acagcggaac agtcgtcacg ggggtatccg
    23881 aatcggatat gttcgttctc aagaccgatt caaatggtga ttcactctgg atccgttatt
    23941 ttggcgggga tttttatgac gctggcgttg cggtcgcgcc gacttctgat ggaggcgcga
    24001 ttgctgtcgg gtatttgtcc acagctgacc agaaatacga ttcactcgtg gtcgtcaggg
    24061 ttgatccttt aggagatgaa gtctggggaa aggtttttcc atttagcgat ggcgaggtat
    24121 ttgatgcgag cgccgatatt tacagagacg aggggggcga atacattctg actgccacgc
    24181 ctgatttcgg accggtcttc tcctacaagg ttgatgaaaa cggaactgtt ctatggtcat
    24241 ctgatctgga tcaggacggc tatttgacgg ttcttgatat ctgtgaaggt cgtaatgagt
    24301 caacgtatat taccgggagc cgcgggccgg ccccacacac actcgtaggc aaaatcgatt
    24361 cggacggaga ttcactcttc ttaagcagtc tagtcttgtc agaaaactca acaggtgagt
    24421 tcattcatca ggatgacagc gggtacatat atgtcggtgg cagattactt caatcgcata
    24481 ccttttatat cgcgaaactg aaagaagaca cagagtccga cattgccgag gatagggcca
    24541 ttactgcgcc tatatatgca ttgtcccaaa actatcccaa tccatttaac ccaagcacaa
    24601 ggattgagtt ttcgttgccg gccagtaccc atgtcaacat ggacatcttc aatattctgg
    24661 ggcagcgcgt gcgaacgctc gtggacgagg accggccggc cggggtctac gcggtagtgt
    24721 gggacgggat agatcaagac ggaggccaag tagcttctgg attatacttc tgtcgcctca
    24781 ttgccggcga ttttgtgcag tcgaagaaga tgctgttgac caggtaacca gcatatcgat
    24841 tcaatcaggg ccgggcattt gggcgggcct gacctttgca gcggttcagc taaagttaaa
    24901 cacggagggg ctttctgcta agacccagtt acgctgacat tttcttggaa gcaggagaaa
    24961 gccatgcgtg tcagagtgtc tatgcattga aacaatggcg agccaataag gatgttgtgt
    25021 ccgttttgtg cccatcgacc gaccaaaacc accgggtcgg atacgacccg acacaaaacc
    25081 ataaaaatac gtaactccat acggttaagc gtgttgcgac gcaacgtgcc gtggtacaaa
    25141 tcactgccat ttaaccggtc ggagaccaca agggtcctga cctacacaag atccggagga
    25201 cgtctgtagc ttatgtgtcc ggctccaagt gcgacctatc tgactagttc acccccgaga
    25261 tgaacccacc ataattggtg ggccacggtt gagaaactcc cggcgaaata cgttttacag
    25321 gtcagggtct ttatggtcct aacggggccg cctgcgtcgt tggccagaat gtcaggacca
    25381 caagggtcct gacctacagc ggatccggag gattgtctcc gaggatggca gagacatggg
    25441 gaccacgagg gttgtgatct gccctccctg catgtccgcc gtcttggcct ggccgtcgga
    25501 gacggtgcgc acaggccggc tgcacccgcc tcgggcgccc ggctgcgtgc gaggcgccgc
    25561 tttccatttt cctcgacaag ctggtctccg ttttatacct tgaccgaggg aacagtcaat
    25621 ccgagtggga gaacagacgt gactgaggcc atccgacgct atttccggtt cgaagaacac
    25681 ggggccacct tccgccgcga gctgatcggc ggggcgacca cgttcatgac catgtcgtac
    25741 atcatcattg tgaacccgaa gatcctcgag gtggcgggga ttccattcgg cccctccatg
    25801 gtggcgacca tcctcaccgc ctttttcggc acccttctca tgggcgtgta tgcgcgccgt
    25861 ccctttgcca tcgcccccta catgggggag aacgcattca ttgccttcac cgtcgtgcag
    25921 gtgctcggct actcgtggca gtcggcgctt ggggcgattt ttatcggcgg cctccttttc
    25981 actctcatca cgattttcgg cgtacggagc tggctcgcca acgccattcc gcagaacctg
    26041 aaaatcggtt tcgcggtcgg catcgggctc tttctcactt tcatcggact gaacgaaacc
    26101 ggcattgtcg gcctgggcgt cgagggcgcc ccggtccatg tcggcgattt cgggacgacc
    26161 tcgattctcc tggccatcgt cggcttcgtg ctcatcgcag tcatgatgct gcgccgcgtc
    26221 aaagcggcgc tgctgatggg cattctcctc accaccgttc tggccttcct cttcggggag
    26281 attcgctggc ccgccgactg ggtcagtctg ccgccgagcc tcggcccgat cttcttgaaa
    26341 ctcgatatcg gggcggccct cacctggggc ttcttctcgg tcattctcac cgtactggtg
    26401 atggactttg tcgatacgat gggcaccctg ctcggactgt cgctgaaagc cggcttgctc
    26461 gatgaccggg ggaaccttcc ggagatggag aagccgatgc tgtgcgatgc gctggcgact
    26521 gtgttcgcgg cgctggtggg gacgagcacg gccggcgcgt tcatcgagtc ggccaccggg
    26581 atccagagcg gcgcgcgcac gggcctggcc tcggtgttca ctgcgcttct gttcctgctg
    26641 gcgctcttct tggcgccctt tctcaccgtc gtgccgcccg ccgcctacgg ccccgcgctc
    26701 atcatcgtcg gcctcctcat gctctcgccg atcacgaaac ttgatttcga tgatctctcc
    26761 gaggcggtcc cggccttcgt cgtgatcgcg ctcatgagtt tcacctacaa cc
//
LOCUS       DAOPOE010000043        26557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2346_length_26557_cov_5.776847, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000043 DAOPOE010000000
VERSION     DAOPOE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26557
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_26557_cov_5.776847"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..9685)
                     /locus_tag="PKM94_07410"
     CDS             complement(<1..9685)
                     /locus_tag="PKM94_07410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD66507.1"
                     /translation="MTAQDIAVVTVNAGALSASLIAGAGGSVDYVSIDRDTTAKMVGV
                     HSNAGGSTSVAAASRALIEGRSATAGGGGLASIAGATSIHTIQGTTEAVIHAGSRQSQ
                     INQDTGFAGNGVSVTATDYAKIDSRAGTYSASLGAGFGASLTVDTIRRNVSALIGDQS
                     QVRSGGDITVKALAAHYISSSATAFAGAVGVGIAGTLAVAAIGGAIDDEAYDEVVKVK
                     SAINDLLDLSGGIDGLLSDDVAEQAEDTLAEHDLTINSAISSGAARKIVAARIDGGTI
                     TASGNVTVKAIRQLGAVLEEGQVAEALLAGVGGSAGFLAIRGRTAAVIGQDAAAGVDN
                     STVAGAVVTAGGAVTLQALTEKYDNDDDDMFLIDVVTGNGGIAGIGSAVGKLLLVSDT
                     DVSIGRSSSITADGKAGISAESDIDTVNVRANGTVSGFYADGDIDVAATGALRSRTVL
                     GTDAAVTAGGNLDVLSIMDVEGLTAAAAGGLGGLVAQGGQKATVAFTAVDATISAVTG
                     ELTAGGAATVYADTASSFKANVYQVADDDAVATDRAKAYVTLSNRNAEDLSRPAVNAL
                     VNLDIIQITADSIAVQALVSRSEAVAKAVVDTRTLGVDADAVADVDLDVLADVTLHVA
                     IFDAPSVTIEAQTLGLNADSESDAEADSGYADVYSEALNFAMQSANIQVNSGVIRTHD
                     LFVYANTLDPASHYGVAAKTAVDAAVGKITTSYTRDGHNQYSAILGGCDVYYDVTYDR
                     NLTFEIDSAGNVVKNDGDIPYNIDSDTGAIVVGSFFYNGSLTPPTARFCVSGSYEIGN
                     TANINCTFYSHGVDTVDIINNSSRDLVLGFIVGDNGEGVEWLVDIVAPYNYSKTNIKP
                     LASYPESLNVYSKGDIVLVLGASLELPTLTVTLESEGDIWFAGSNRVEARQLEMHAGG
                     NIGTAAKPLYLYTGGPISIAAGGDLYLEVVPVFFYSAFETLKFAELTAGGILSASVFS
                     AVDGDGNAFLDGDGMAIATKVLFSGPVSAGGALTLEVDNSGGHVDFRDEVTAGGDIGI
                     TAEGLIQLFSTITTPGKVTMVSTGGISFFNGLITAHDVDLHTDTYHLANVRVHLTGGA
                     VSAVALDGYAQITSVGGPLTMAKLFVGNGLAILTAEDTEALDDMIVLAAGSALNVKNG
                     IWLNAGDGIQVLGEGTSRGIITTEGEIHITLDATPDLPGNATVAYTDPDPGVGATFTF
                     DSGVYQLTGTSKTVRLQTGADADTILLPVLAEEDYVLSMATGAGDDNLTVGRQAANGT
                     WSTDLVKGQILFYSEEGYDTISIDNSGVSMGEEAGSLVLTDTQEMLYTGPSIAMSGMS
                     KLWYLGVESLAIKLAHAETNTVAIDATGEEMETVSILGSFGNEDFIVGGASTHLLSSV
                     HGVLTLNGGSDDFGTDTLAIVENNFLSGLSFPDDVLTATSFTGQGMDGVHYLGMESLS
                     LTFGSSDDTLVIEDTPQYVPATIDLGGGDDAVFIGGGNLETGFGGTLTLDGGADDDTI
                     AYDDSLYAEAYTAFLNGALFARSLADGSNTLTPAISSTGFEATTILLGSGGGTLDVKA
                     LPTGTTRIVGGAGADDITLEAGPASGSILDISAGAGDDTLRAMGSTVLASLAGAVFFA
                     GGEGSDQIRLSAGDAAAGTNLFLKSPDSLPLLHSGETGWALIQGLGMGGGIFYGAEDL
                     EITLTNHADTIELTATSATTDSVALYGEAGNDAFYAVGGANGLDELHGDFELRGQEGT
                     DLFWVQNQAATKDFDTGEAYDAVYGLAGFSGQGLTGKIIDIDIEDVAFYLGSGKDSLA
                     LDFISGGKAMLVAMAAGDDRIRISPTYANEITLSGGDGTDLISLDDSADSTNRSITVT
                     GRNFTGLLSGSAEIMVATDIEAAELLLGGGYVDVTINGATLPLVVKGEEGAYTYTVTS
                     LDAGQQVDLYGGDGWYDKLYVGDQDGSASVYGTVNFHAGATGSEVYVYNLAASGALWV
                     EGGAGSDSLALDGGATGTVQYIDGAIVFAGNGGTDYVTVVETTSASVTYPLTFDKSEA
                     PDGSTMAHIGGLGMGKGIWYAAEDVGAWLEDATTTPVNVVATAAENTRLQVMVVNAAA
                     QFYLGTTKENYGSAVPGSLANLHGSIDLLGSGEGTMVIDDSSATAGWENAVVNATTAS
                     GMWDGSLSYDGVNFLTILPGTGADRIHLDAIPNWYSGSTKVNGLGTNDVVFTSPDVVN
                     QYEDGNNYWRLNNANALGVRVTENQTFVEQNPSATIEIFKNFTTKDISDLENDVWVSS
                     GRIEYVSTTHIDNDYAQFLFTYRWISTTAADEDVEVYVLDDDNYDDDKVRLVTAARFH
                     VDVQDVAPTVTLADQTASEGSVSLAGSFSDAGFRNGETYTALWQILDPTTGELVQEVA
                     GSYSRNGSEYNAQIAGSVDLAMGTYTLSATVTDSDGMATTKTSILTVANDAPVVNAGL
                     DRNVEGLTTTLNGTFTDAGQSFGEGYTATWEVTNSDGETVASGDDLDLNFTVADYGTY
                     TATLTVTDSHGVAASDSCQVAFAANFAPVVEAGADVTVDIAHTTTLTGAWQDPGTPAA
                     VGWTVVNASGVEVDQVSGATLTFGPAALGTYTATFSVTDSSGVTSTDTLTITVANFAP
                     VVNAGADVTVDVGSQASVSQLINFTGSFVGTDGKTYTSADSVYERVENGWNRYFYRTT
                     DGKIYTCNWEFYNITGKDPDDPATWPSPHTGLFDSSPTCLDVHNTWNFVYTETFALKL
                     TVTEVYTDASWNITGTGASASDYMLVKVTADDWITLESANAINALVGNNTSGAYFGLY
                     KMRSPVDASAVTGIVDDLNQTTFIDWGDGTSTAARVDLSQTIPVGQYNLVYLVVVVWG
                     DSSTAETRHFYSAGSYDQADGVYPVTVQSTWMKSLTIHTTATVQSVTPVATVTPDIVA
                     AGKETAVRVQFGDQSGLTSDLSLHVTWGDGTEEVYTVAFEADTKYAVDLTHTYAEAGE
                     YIPHFYYTRTDTAGATVTGATGHGHITVTEEAPLTIANLPETCTEGTALQLTAAVDHT
                     IAGPFTYAWTVTKNGAAYATADTKELTLTPNDEGTYAITLLVTDERGSTRKASGILAV
                     ANVAPTVSIAGLPATSTEGTAISLTSTVNDPGTADVLTYAWSVTKNGSAYQSGTAGSF
                     TFTPDDNGDYVVTLTVSDGDGGSGQATGTVTVGNVAPTVSIVGLPAESPEGTQISLTS
                     LVTDPGATDTPTYAWSVTKDGTVYGTAGTGATYDFTPTDDGTYVVTLTVTDK"
     gene            complement(10167..24992)
                     /locus_tag="PKM94_07415"
     CDS             complement(10167..24992)
                     /locus_tag="PKM94_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HOD66508.1"
                     /translation="MWSLGRKKTSAEKTEKKARRKDRRPFQLEPLEPRLLLSADVPLP
                     VGDIKEAPGIVPAVVESVTVTPADTVQAADELNPAIRQNGPPAENAENIDQPLTAAVP
                     GSDQADRQPPAFSPGKIPGANPAQEKSKADISTPLPAAQSAAEEAVSDYSALSGEKRI
                     LRELVIVDSSVHDYESLVTELKAKIAAENTQSTEIEWTICVLTANTDGIEQISEILKS
                     YQDLDALHILSHGGTGEMSLGSTAVLQEVLQSREAEISAWADSLKDDGDILLYGCDVA
                     AGAAGIEFVETLAGITGADVAASTDTTGSADGGNWELEYVVGTVETGTVFTGADFAYG
                     YLLQDIVGTDADETLTGTDEGDRYVFDDGWGTDTMSDPGSSGTDILDFSNVEADLTFA
                     VATDGTLSVTDGTNTLSGVSGIEKILYGQGENLFSYSGGVSEKIKDFFTMGFLVGDDA
                     DLKMVLTLGPDEVTLAAGLELGKLRLYNDENEFIFAAPTSSLTIQMGLGNDEFTVGDL
                     GEIGYDLTINGGYGTDSGWVDGEDSIVFAASNEFDGRDIEANAETVTVQAGVALVTTG
                     AAGQSAGDIAFSGSTVDVKDNARLITVGQAGGAPGDITLTAQVTDLSGLSPVDVFPAD
                     DVVVRVGAAELRGGAIEITANRTSMTGSPMTIVSVQSKTATIDLKNAVISGSEVFIEA
                     SAEDQSLFDSPGGNIFSNYLLTPLLNYGDVIGLLMGSIPIAGAALQAVSVSVRTAESY
                     VTLDGASIQSTGNVTIHTSTSVESAASAGGGIDLTKPKRGNSTASQLANSAPYAVSYA
                     EADAVAETVLSGCTTIAAGGTVTVGSDAENGVSAQSYTAANSAPGGKKTKTNTSVGAT
                     SIAVTNASTTSLTTVDSSVVITAGGNANIAAVGQVENSADSGVSIFVDGFGGVGLALA
                     IDDTDIKAEINGKVTAAGESTAISLELANVNTANDTITIPDHGLKTGDELLYLAADPD
                     SGITVSSIGGLESGTTYRVVVVDENTIQLVRGQTIEFDTTATLNVNPDSVQSVSRKAT
                     ISFEPATAVDVTQNTITFGTAHEFTTGQVVDYYVASDAGEAIGGLENEGTYVVAKVDA
                     DTIKLATANPEDEDVTGETFTCNGTTYYVIDLILTEGSATKSTITFDPTTADADTGAT
                     AVSGNEIVFADTHAFTTGQRLVYATGGGAAIGGLVDGGTYWAIVTGTDRIQLALSAEA
                     ADAGTAIALDGTRASGTAHSFTGYHTHLLSYVQTALTFKVVDAVDTENGAIDFGYNHG
                     LSTGDAVVYATDPSFATEVDIDRGTQFSVADSTVAFNPSGIIDEEAVVDADGSTIVLG
                     LIHDFVTGERIHYDTGGGTAITGLTGDTDYFVIASGDDRIQLAATRADALAGIAITDL
                     SAAGATGSGHTLTANAVDIADNLLVITNQEFATGQEIVYAVGEGGTAIGGLVSGQTYY
                     AIRGEGDILQLAASQQNASAGIAIDFTSVHTAGMQGFSASTVVCTFDGSRTVSSLDLA
                     AATIEIAAHGLSAGQLVTYSASDGEVIGGLTEGADYYIITVDANHIRLAASADDAANG
                     KAVALTSIGAIGIDLHALTTWDADGNEIATLYFDPTLKPAVVEGGIWNPNHGYETGEL
                     VTYLAGGGTAIGGLQDGHAYYVIVLDANRFGLADASNPANDPANPGSLAAVTLVGGVA
                     TGEVHGFERASTGTRHDVAIGGLTAGEIYFVTVVDSHTIRLSESLLTALAAAPVDLTL
                     SAEQQAGAATLTHTLQAPNKKAGINVVASLEASNSAVVKSSVGAIPSLSDIIGGNVPP
                     DPKVLKSVVKGAGKSYNADKKTAVSVAASVGVSVFDHDVTATVGPTAVLKSGADVTIS
                     ASAEEGASVNAQGNISTEATNSKKSKGTGGGGKKVAAVSLAIGVGAYDSNVQTIVKGG
                     AAIDARQTVSVLSDLSYPLLVDSIPFNPAGFDIAQDSIIDQVAGLLDGQLGMTGVFNM
                     WVTTTGRAPDSQLTITGSVGMTFYQNHSEALIEAGALINQDNTYWNADQSVLVAATTE
                     MELINIAGVVQLKLNDQGIQNLTKARKIGGRSSPISLFGNEAKTLGIGGSVLVQSIDN
                     ETIAQIGASAVHAGANGAVTVSAAEDIFTFDYVQTGGNSGKAGVSGSFALVEQTSDTV
                     AQIASGAKITGGDLAVDAESSVTHLNFTGAVQLARQLGIGVSSGTTLLERNTEALIGN
                     RLTATDTAVGNAGTVLDVAALSLSAVNSGDILVIGVAGAAVMDVPGLTIKDTTGAGAQ
                     PAAIGAGIAGVVSIAEVDDLTRAYINDSASLDLNYGAIELAATDSTAVQTLAGAAAFA
                     KTSKTAVGIAGAFSYSGLEMTTEALITGAQMNSAGAVALNAERTGNILSLAIGVAIAA
                     TVPTAGGSGTGTGVAGAVTGSAAINTVSGSTAARLKNANLSSPRSVIINATDESDISS
                     VAIGGAGAGGDSSAVAITGALALNVIDTSIEALIDSSRVTASGAVQLAATDNTAISAD
                     AGGVAIAISAKKTGGAATTGAVAVGAGAAANEIGQKSAHTVHALIEDSYITATADVEL
                     TAVANATINALAIGGAGAAQTGGAGAGTKITLAGAGAGSGNAVNFDIKAAISADREVT
                     AGGSGGILLTASNQSKIYADAGGVAFALTAGAATGSTVTASVGVAAALNDIEQSVVAT
                     IDDSPAIAVGDVALSAASDLTVQAFALGLAASGSSGGGTGMSGTLAGAGSGAVNTLNS
                     NIEASITDGSEAQAQTGSVGITAADRTKVRADAGGVAIAVQAGGGSGSSGSLAFGASV
                     SLNDVADTVSALVDNSWIHAAKNIDLAASSKIDVDALTLAGSGSVAAGGGSSIGAAIA
                     GAGAGSGNSIANTTNAIVKGGSALQSATGSIGITATDAAAITADAAGGSVVVSAGSQN
                     SGAVAVGVSVAVNEIADTVRAAIENSTITKVDDLTVAADAQGSIDAVSVAAAVSVAAG
                     ATTGVAVSGGGAVATNYIGTDVAGYLSGANVTSTGNITVTADNTAEVNAEVAAISAAV
                     GVGPQNGGAAAIGVSVARNFVGWSPDTDTDYDYSTEDGLGRGQTLQSGDCVLIESGAR
                     AGDVYEYLGESRTQATAKYTTASGKRKLASGDIVSIAQNYTGAAITGSLYRYIGSGAT
                     VNLAKADYLGDDWEACWIDLQTEDYSDTTVWKQVNLAEGPALTQAYAAGGKIAATGAL
                     TIAATAAESIDAVVATAAVAVAGGGQTGVAVSAAGVFAENRINTDIDGYISGGDIDTG
                     SISVTADDSSAINSVAAAASISGAYGGTAGVAVSVGLSLAYNEIANDVSAYIKNADAT
                     ATTGAVTVAATASGEHLFDMDLTGLSLAASDLDDAAAAAGDSADSAGDAAILEKIRSA
                     FAAQGETLAVTDSVTPASLYSTGAGVKVLVNGDTVRIEEGYTGGGIEGRVYRYKHVQP
                     LHVNLGSENYANFARWELVTAELTLTTLVEGQSWSLVDGSGATYLLHANGNALEVTRG
                     TINAVSAAASLAATYGGTAGVAVSGAGAFSANSVLTRVNAYAQSSDLVSAADVTVSAA
                     DTAAVSSAVLAASTAIAGGGIAGVGASIGASLAWNLIGSDLAGNSRPAQVRAYVTDST
                     ISAAGDLQQTAISSQSVSSIVLAGSVALAASGTVGIGVSGSGVWAENRIATDIAAFID
                     TSANISADTITLNARDDSLIRALAGAVAIAQAYSGTMSAGIALGVSAAQNVIANSVDA
                     YVNDAALLQATAGPIVIGAAETAQISACAFAASIAASGSGVVGVGVSGAGAEATNAIL
                     TGVHAFLAGSAAQSTGNVTIEALNSSTLQALIASVSASVGGGTAGVGVAIGASVAQNY
                     IGYDENGARVADSAQVQAYLQETELHAGGGVTLKAQAAQEIEAIVLAGSVAVSGGVGG
                     FSGSGSGVWAQNKIASAVAAFIDGSNGITADTGNIILNAQNTSSINANAEAASVAASI
                     SIGGALSIGASLAFNEIANHVSAFIDDAAYVKALQGGISVTAADDSSILSSSVMASVA
                     AGLVGVSGGGASSTNAITNTVTARIANETGITEVLAGRGAVTVSAVSNSTIASLAGAL
                     NVGAGAGGVAIGAAVGVNRIENSVTAAIEKASVTAVAASGMENSGDVTVSATSTPVLI
                     GMSLAGSFSTTASLAGSLTINSIANTVDAHIADSAIEADGDVLVTAAETPVLYSVAGM
                     AALGGAAVGASLAYNYVGGSIDPDDPNVLTYTETDEGMSKPAVTGSTGARTSGVTAAI
                     TGSTVDAQGAITVSASFGDPESLPADDVLDGIELPVSIEAGITGVTVAGSGAAAFALG
                     GAVTLNFLRAEVSAQIADTPTGGKIAAGGDVIVSALDESQVTGLAGTLAGAGAAAIGA
                     SVVVNDIRNSVSSAIAGAAVDAAGKVAVLAAEEAIDLNVAVGGAAGGGVAIGGSVSVN
                     RIDNTTTASLSANGGKESDVRATGDITLSATDRSAISTIAGNAGAAGAVAAAGAFSFN
                     RIGSSTEAVLSDSRARSTAGAITVSATAGPPATLPEGLSAQISAVSVSGVGAGIAGAA
                     GSVSVNDIDATVEAVISHSTVSAAGSFTVDAADSSTIDSLAGAVVIAGGGGIDAAVAT
                     NDIANRVKARITDGSAVTAGGAAQVSASSESAIRSLAAGGAGAAGFAAGGSVTLNEID
                     NTLRADVWDSDLTAQGIEISAQSDSTIESLAGSIAVSGIASVGAAVATNDIADDVYAT
                     VRRSNLDAGAQELEITASETSTIEAAAAGFAVAAYSLAGGVALNSTNNTVEAEVTDDS
                     TVTAGAITIEATDSAKIEVRAGAVAAGLAWGLGGSAAYNEIADTVRAKVADSTLTATD
                     GSIAVLATAEGDIDAVAAGGSGGGHYCRPDGFQYRGSAGGGKHPRCFRHGPGRRRLGG
                     RYRCRRRHRLPGRHCRHRRHHRPEHAAQQRQGRHQRLHGHGRRQRNRKSSDPLEPQGQ
                     RKRLGRRPVAGDRPVCQGRRRHCRR"
     gene            complement(25008..26420)
                     /locus_tag="PKM94_07420"
     CDS             complement(25008..26420)
                     /locus_tag="PKM94_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOD66509.1"
                     /translation="MLKMKWLRIMMISNHYKSMGKPSAFCWRELVTLLAVLFLVPLSA
                     AQAEGILDIYQQAIKNDPAYQGAQFSHDASKETLAQARSAFLPKISAEGAYTGTHQNV
                     GRSDNTSYTPGSSSYPTTEYSVSLIQPLFNKSSLSNLFRAQAKVKGADMELEAARQDL
                     IVRVTKVYLGVLAARDKLNLAQTEEASIRMQYELAYSKYSLGLARKTDYLDAKARLAE
                     VRANRIAAESALDGALQALREVTGKKAASLAPLRSDLPLKRPEPGEADAWIAAAVKQN
                     PAIEVQRQSVEMALQEYKRQVAGHYPVLNLEAKYSNIDTQGTLFGGGYEAGTSNVLVR
                     LNFPIFEGGVVNSRSREAYNLYQASLQEQERQLRALQKETRAAYHGVLDAMERVAALR
                     EAVEAQELLVQAKQDGYRSGLYILVAVLDAQRDLTRGRQDYATARYDYVMNSLRLKKA
                     AGTLAEGDMVTVQGWLEDSK"
BASE COUNT         4425 a         8427 c         8573 g         5132 t
ORIGIN      
        1 ccttgtccgt gacggtcagg gtcaccacgt aggtcccgtc gtcggtcggg gtgaagtcat
       61 aggtggcgcc ggtcccggcg gtcccgtaga ccgtgccgtc cttggtcaca ctccaggcgt
      121 aggtgggcgt gtcggtcgcc ccggggtcgg tcaccaggga ggtgaggctg atctgcgtcc
      181 cttcgggact ttcggccggc aggccgacga tcgagaccgt gggagccacg ttgccgaccg
      241 tgacggtgcc cgtggcctgg ccgctcccgc cgtcgccgtc ggaaaccgtc agggtgacga
      301 cgtaatcgcc gttgtcgtcg ggggtaaagg tgaagctacc ggctgtaccg ctctgatagg
      361 ccgaaccgtt tttcgtgacg ctccaggcat aggttaacac atccgccgtt cccggatcat
      421 taaccgtgga agtaaggctg atggccgttc cctcggtact cgtggccggc aggcccgcta
      481 tggagaccgt gggggcgaca ttggcaaccg ccaggattcc gctcgccttg cgggtgctgc
      541 cccgttcgtc ggttaccagc agcgtgattg cataggttcc ttcgtcattg ggggtgaggg
      601 tcaactcttt cgtgtcggca gtcgcataag ccgcgccgtt cttggtcact gtccaggcat
      661 aggtgaaggg gccggcaatc gtgtggtcca ccgcggccgt gagctggagc gccgtcccct
      721 cggtgcaggt ttccgggagg ttcgcgatag tcagcggcgc ttcctccgtg acggttatat
      781 ggccgtggcc cgtagcgccg gtgacggtgg caccggccgt atcggtccgc gtgtagtaga
      841 agtgcgggat atattcgccc gcttcggcat aggtgtgggt cagatcgacg gcgtatttgg
      901 tatccgcttc aaaggccacc gtgtaaacct cttccgtgcc gtcgccccag gtcacgtgga
      961 ggctgaggtc ggaggtgagg cctgactggt cgccgaactg gacccggacg gccgtctcct
     1021 tgcctgccgc aacgatatcg ggggtcacgg tcgccaccgg cgtgacggat tgcaccgtgg
     1081 ccgtggtgtg gatggtgagc gatttcatcc aggtgctctg aacggtgacc ggatagaccc
     1141 cgtcggcctg gtcatagctg ccggcggaat agaaatggcg ggtctcggcc gtgctcgaat
     1201 cgccccaaac cacgacgacc agatagacca gattgtattg accgaccggg atcgtctgcg
     1261 acagatcgac gcgggccgcg gtgctcgtcc cgtcacccca gtcgatgaac gtggtctgat
     1321 tgagatcgtc cacgatgccc gtaacggcgc tcgcgtcaac gggagagcgc atcttgtaaa
     1381 gtccgaagta ggcgccgctc gtgttgttgc ccaccagggc gttgatggcg ttggccgatt
     1441 cgagggtgat ccagtcgtcg gccgtgacct tgaccagcat gtagtcgctg gcactcgccc
     1501 cggtgccggt gatgttccag gaagcgtctg tgtagacctc ggttaccgtg agtttgagcg
     1561 cgaacgtctc tgtatagaca aagttccacg tattatgcac atcgagacac gtcggcgacg
     1621 aatcgaagag acccgtgtgc ggactgggcc acgtggcggg gtcgtcgggg tccttgcccg
     1681 taatgttgta gaactcccag ttgcaggtgt agatcttccc gtccgtggtg cggtagaagt
     1741 agcggttcca accgttttcg acgcgctcgt aaaccgaatc cgcgctggta taggtcttgc
     1801 cgtccgtgcc gacgaagctc ccggtgaagt tgatgagttg agaaaccgac gcctgggacc
     1861 ccacgtcaac agtcacgtcc gccccggcgt tcaccaccgg cgcgaagttg gccacggtga
     1921 tcgtcagggt gtcggtggag gtcacgccgg agctgtccgt gaccgagaag gtcgcggtgt
     1981 aggtgccgag cgcagcgggg ccgaaggtca gggtcgcgcc ggagacctgg tcgacctcga
     2041 cgccgcttgc attcacgacg gtccagccga ccgcggccgg tgtgccgggg tcctgccagg
     2101 cgccggtgag cgtggtcgtg tgggcgatgt cgaccgtgac atccgcacct gcctccacga
     2161 ccggggcgaa gttcgccgca aaggcaacct ggcaagaatc acttgcggcg acaccgtggc
     2221 tgtcggtgac cgtcaatgtc gcggtgtagg tgccgtaatc cgcaacggtg aagttcagat
     2281 caagatcgtc gcctgatgca acggtctctc cgtcactgtt tgtaacctcc cacgtggccg
     2341 tatagccttc gccgaaactc tgccctgcat cggtgaaggt gccgttcaag gtcgttgtca
     2401 gcccctcgac gttgcggtcc aggccggcat tgactaccgg cgcgtcgttg gcgaccgtca
     2461 ggattgatgt cttggtggtg gccatgccgt ccgaatcggt gacggtggcc gatagggtgt
     2521 aggtgcccat ggcgaggtcc acagagcccg cgatctgggc gttgtattcg gaaccgttgc
     2581 ggctgtagct ccccgcgact tcctgcacca gctcgccggt ggtggggtcg aggatctgcc
     2641 agagggccgt gtaggtctcg ccgtttctga agccggcgtc gctgaaggag ccggccaggg
     2701 aaacgctccc ctcggaggcg gtctggtcgg ccagcgtcac cgtaggggcc acgtcttgga
     2761 cgtcgacatg gaaccgggcc gcggtgacca gtcgcacttt atcgtcgtca tagttgtcat
     2821 cgtccagaac ataaacctct acatcctcat cagccgccgt ggtggaaatc cagcggtagg
     2881 tgaagaggaa ctgggcataa tcgttgtcga tatgcgttgt gctgacgtac tcgatccggc
     2941 cgctggagac ccagacgtcg ttttcaaggt cgctgatatc cttggtcgtg aagttcttga
     3001 agatttcgat tgtggccgac ggattctgtt cgacaaaggt ctggttctcg gtgacgcgta
     3061 cgcccagggc gttggcgttg ttgagcctcc agtagttgtt tccatcctcg tactggttga
     3121 cgacgtcggg ggaggtaaag acgacatcgt tcgtgcccag gccgttcacc ttcgtggagc
     3181 cggaatacca gttggggatc gcatccaggt gtatccggtc cgcgccggtg cccggcagga
     3241 tcgtcaggaa gttgaccccg tcataggaga gagaaccgtc ccacatgccg gaggccgtag
     3301 tggcgttgac gacggcgttc tcccaccccg ccgtggcaga agagtcgtcg atgaccatcg
     3361 tgccctcgcc cgaaccgagc aggtcgatgg agccgtggag gttggccagg gagccgggga
     3421 cggcgctgcc gtaattctcc ttggtggtgc ccaggtagaa ctgggccgcc gcgttgacga
     3481 ccatgacctg gagcctggtg ttctccgccg cggtggccac gacgttgacc ggtgtggtgg
     3541 tggcatcctc gagccaggcc cccacgtctt ccgcggcata ccagattccc ttgcccatcc
     3601 ccaggccgcc gatgtgggcc atcgtgctgc cgtcgggggc ctccgacttg tcgaaggtaa
     3661 ggggatatgt cacggaagcg gatgtcgtct ctacgacggt aacgtaatcg gtcccgccgt
     3721 tgcccgcaaa aacgatggcg ccgtcgatgt actgaaccgt cccggtcgca ccgccgtcca
     3781 gcgccaacga gtcgctgccc gcgccgccct cgacccacag cgcgccgctg gcggcgaggt
     3841 tgtagacgta gacctcgctc cccgtggccc cggcatggaa gttcaccgtg ccgtataccg
     3901 aggccgaacc gtcctgatcg ccgacataga gtttgtcgta ccagccgtcg ccgccgtaga
     3961 gatcgacctg ctggccggcg tcgagcgacg tcacggtata ggtgtaggcg ccttcctcgc
     4021 ccttcacgac caggggcagc gtagcgccgt tgatcgtcac atcgacatag ccgcccccga
     4081 gcagcagctc cgccgcctca atatcggtgg cgaccatgat ctcggcgctg ccgctgagga
     4141 gtccggtaaa gttccggccg gtgaccgtaa tactgcggtt ggtcgagtcg gcgctgtcgt
     4201 ccagggagat gagatccgtt ccgtccccgc cggaaagggt gatctcattg gcataggtgg
     4261 gagaaatgcg gatgcggtcg tcgccggcag ccatagcgac cagcatcgcc ttgccgcccg
     4321 agatgaaatc cagggccagg ctgtccttgc cgctgcccag atagaaggcc acatcctcga
     4381 tgtcgatgtc gatgattttg cctgtcagcc cctggccgct aaagccggca aggccataga
     4441 cggcgtcgta ggcttctccg gtatcgaagt ccttggtggc ggcctggttc tggacccaga
     4501 acaggtccgt gccctcctgg ccccggagtt cgaaatcgcc gtggagctcg tcgaggccgt
     4561 tggccccgcc gacggcgtag aaggcgtcgt tccccgcttc gccgtagagg gcgaccgaat
     4621 cggttgttgc actggtcgcc gtcagttcga tggtgtccgc gtgattggtg agggtgatct
     4681 cgagatcctc cgccccatag aagatcccgc cccccatgcc gagcccctgg atcagtgccc
     4741 agccggtctc cccgctgtgg agcaacggta agctgtccgg cgacttgagg aagagattcg
     4801 tcccggccgc ggcgtcgccg gcggagagcc ggatctggtc gctcccctcc ccgccggcga
     4861 agaagaccgc gcccgccagt gaggccagca ccgtggaccc catggcccgc aaggtgtcgt
     4921 ctcccgcgcc ggcgctgatg tccaggatgc tgccggatgc gggacctgct tcgagggtga
     4981 tgtcgtcggc cccggcgccg cccacgatcc gggtggtccc ggtggggagg gccttgacgt
     5041 cgagggtccc gccgcccgag cccaggagga tcgtcgtcgc ctcgaatccc gtggagctga
     5101 ttgccggggt cagtgtattg ctgccgtcgg cgaggctccg ggcaaagagc gctccgttga
     5161 ggaaggccgt ataggcctcg gcgtagaggc tgtcgtcgta ggcgatcgtg tcgtcatccg
     5221 cgccgccgtc gagggtaagg gttccgccga aaccggtctc caggtttccg ccgccgatga
     5281 agaccgcgtc gtcgccgccg cccaggtcga tggttgcggg aacgtactgg ggtgtgtctt
     5341 cgatgacgag ggtatcgtcg ctcgagccga acgtcaggga gaggctctcc atgcccaaat
     5401 agtggacgcc gtccatcccc tgtcccgtga agctcgtggc cgtcaggacg tcgtcgggaa
     5461 aactcagccc ggacaggaaa ttgttctcca cgatggcaag cgtgtcggtc ccgaaatcgt
     5521 cgctgccgcc gttgagggtg agcacaccgt gcaccgacga gagcaggtgg gtggacgcgc
     5581 cgcccacgat gaaatcctcg ttcccgaaac ttccgagaat cgagacggtc tccatctcct
     5641 cgcccgtggc gtcgatcgcc acggtgttgg tctcggcatg ggcgagcttg attgccagcg
     5701 actcgacacc caggtaccaa agtttggaca tgcccgacat cgcaatcgac gggccggtgt
     5761 agagcatctc ctgcgtgtcg gtgaggacaa gactgccggc ttcttcgccc atgctgacgc
     5821 cggagttgtc gatagagatc gtatcataac cctcctcgct gtagaagaga atctgtccct
     5881 tgaccaagtc ggtgctccag gtgccgttcg cagcctgccg gccgacggtg aggttgtcgt
     5941 cgccggcccc ggtagccatg ctcaggacgt agtcctcctc ggcaagtacg ggaagcagga
     6001 tcgtgtcggc gtcggcgccg gtctggagcc gcactgtctt gctcgtgccg gtaagctggt
     6061 agacgccgct gtcgaaggtg aaggttgcgc ccacgccggg atcgggatcg gtataggcga
     6121 ccgtcgcatt gccggggagg tccggcgtcg cgtccagggt gatgtggatc tcgccctccg
     6181 tcgttattat gccgcggctc gtgccctcgc ccaggacctg gatcccgtcg ccggcgttga
     6241 gccagatccc gttcttcaca ttcagggccg atccggcggc caggacgatc atgtcatcca
     6301 gggcctccgt gtcttcggcc gtcaggatgg cgaggccgtt ccctacaaac agcttggcca
     6361 tcgtcagggg accgccgacg ctggtgatct gggcgtaccc gtccagggcc acggcgctca
     6421 cggcgccacc ggtgaggtgc acccggacat tggccaggtg gtaggtatcg gtgtggaggt
     6481 cgacgtcgtg ggccgtgatc agtccgttga aaaaactaat cccgccggtg ctaaccatgg
     6541 tcaccttgcc cggggtcgtg attgtgctga ataactggat cagcccttcg gccgtgatgc
     6601 cgatgtcgcc ccccgccgtg acctcgtccc taaagtccac gtgacccccc gagttgtcga
     6661 cttccagggt caaggccccg cctgccgaaa ccgggccgct gaagagcact ttcgtggcga
     6721 tcgccatacc gtcgccatcc agaaacgcat tgccgtcgcc gtccacggcg gagaagacgg
     6781 acgcgctcag gatgccgccc gctgtcagct cggcaaactt cagggtctcg aaggcgctgt
     6841 agaagaagac cggcaccacc tccagataga ggtcgccgcc ggccgcaatg ctgatgggcc
     6901 ctcccgtgta caggtataag ggcttcgctg ccgtgccgat gttcccgccc gcgtgcatct
     6961 cgagctgtct cgcctccacc cggttgctcc ccgcgaacca gatgtctcct tcactttcca
     7021 gagtgacggt caacgtaggc agttcgagcg atgcgcccag gacaaggaca atgtcgcctt
     7081 tcgagtagac attcaggctt tccggatagc tcgctaaggg cttgatattc gtcttggaat
     7141 agttgtaggg ggcgacaatg tccacgagcc attctacccc ttcgccgtta tcccccacga
     7201 tgaagccgag gacgaggtcg cggctggagt tgttgatgat gtcgacggta tccacaccat
     7261 gggagtaaaa ggtgcaattg atgttcgccg tgttgccgat ctcgtaggac ccgctcacgc
     7321 agaagcgcgc cgtaggcggt gtcagggagc cgttgtaaaa gaaactgccg accacgatcg
     7381 cgccggtgtc ggaatcgata ttgtagggga tatcaccatc gttcttaacc acattgcctg
     7441 cggagtcgat ttcaaacgtt aagttccggt cgtaggtcac gtcgtaatag acgtcgcatc
     7501 ccccgagaat cgcggaatac tgattatggc cgtcgcgcgt gtacgaggtg gtgattttgc
     7561 cgaccgcggc atcgaccgcg gtcttggccg cgacgccgta gtggctcgcg ggatcgaggg
     7621 tattggcata gacaaacagg tcgtgcgtgc ggatcacgcc gctgttcacc tggatgttcg
     7681 cactctgcat cgcgaagttg agggcctcgg aatagacgtc ggcatagccg gaatcggcct
     7741 cggcatcgct ttcggagtcg gcgttgagtc ccagggtctg agcctcgatg gtcacggacg
     7801 gtgcgtcgaa gatggccaca tggagcgtca cgtcggccag gacatccagg tccacgtcgg
     7861 ccacggcgtc ggcgtccacg cccaaggttc tggtgtcgac gaccgctttc gcaaccgctt
     7921 cgctgcggct gaccagggcc tggaccgcga tgctgtccgc cgtgatctgg ataatgtcca
     7981 ggttgaccag ggcattgacc gcgggacggg agagatcctc ggcgttccga ttggaaagcg
     8041 tcacataggc cttggcccgg tccgtggcca ccgcgtcgtc gtcagcaacc tgatacacgt
     8101 tggccttgaa ggaggaggcc gtatcggcgt agacggtcgc ggcgccgccg gcggtcaatt
     8161 ctccggtgac cgcgctgatc gtggcatcca cagcggtgaa ggcgaccgtc gccttctggc
     8221 cgccctgggc cacgaggccc cccaggccgc cggccgcggc ggcggtcaga ccttccacgt
     8281 ccatgatgct caggacatcc agattcccgc ctgccgtgac ggccgcgtct gttcccaaga
     8341 cggtccggct gcggagcgcg cctgttgcgg caacatcgat gtctccgtcg gcatagaaac
     8401 cgctcactgt gccgttggcc cggacgttaa cggtgtcaat gtcgctttcc gccgagattc
     8461 cggccttgcc gtccgccgtg atggagctgg acctgccgat ggaaacatcc gtgtcgctga
     8521 cgagtaaaag cttgcccacg gccgaaccga tacctgcgat gccgccgttg cccgtgacga
     8581 catcgatcag gaacatatca tcgtcatcat tgtcatattt ttccgtcagc gcctggaggg
     8641 tgacagcgcc gccggctgtg actacggcgc cggcaacggt ggaattatca acaccggccg
     8701 cggcatcctg gccgattacc gctgccgttc tgccccggat agcgaggaat ccggccgaac
     8761 cgccgacacc ggccagcaac gcttcggcaa cctggccctc ttcgagaacc gcacccagtt
     8821 gacggatggc tttcaccgtc acattaccgg aagcagtgat cgtacccccg tcaatccgcg
     8881 ccgccacgat ttttcttgca gcaccggaac tgatggccga gttaatggtc aaatcatgct
     8941 ccgccagggt atcctccgcc tgctctgcca catcatccga gagcagtccg tcgatgcctc
     9001 cggagagatc cagcaggtcg ttgattgcgc ttttgacctt gacaacttcg tcgtaagcct
     9061 cgtcgtcgat cgcaccgccg atggcggcaa cagccagcgt cccggcaatc ccgacgccga
     9121 cggcgcccgc aaacgccgtg gccgaagagg atatataatg ggccgccagt gccttcacgg
     9181 tgatgtcccc gccggaacgg acctgcgact gatcgccgat caaggcgctc acattgcgcc
     9241 ggatggtatc gacggtcagg gaggccccga atcccgctcc caggctggcc gaataggttc
     9301 cggcccggct gtcgatcttc gcgtaatcgg tcgccgtcac actgacgccg ttgcccgcga
     9361 atcctgtatc ctgattgatt tgggactggc gtgatccggc gtggataacc gcctcggttg
     9421 ttccctgaat agtatggatg gaagtggcgc cggcaatcga ggccaaaccg ccgccgccgg
     9481 ccgtggccga acggccttca atcaaggcgc ggcttgcggc cgccacactg gtgcttcctc
     9541 ccgcattgct gtgcaccccg accattttgg cggtggtgtc gcggtcgatc gagacgtaat
     9601 cgaccgaacc gcccgcaccg gcgatcagcg aggcggaaag ggcgccggca ttaaccgtta
     9661 cgacggcaat gtcctgcgcc gtcactgcaa taccgcccgt ttcggtcgta atgtcggctt
     9721 ctttgatgaa agcctttgta tctccggaaa tggaaacggc ggagacggcg ccggccgccc
     9781 caaccagatt cgctgccgcg ctacccgtcc gagaaaagac atattcctgg ctccaggcac
     9841 ttacctccac accgcgggaa ccagcctgta cgctctttat gtccgttgta ttgctgattg
     9901 atgccgtaac ggccgtatcg ataatggtcg tgtcgaagcc gcccacgacc ccgaggccgt
     9961 acacgccggc cgcgccgccc tgattgttca cagcggttac ctgcccggcc cgaacgaaaa
    10021 caccgcccgc tccgatcaga ggctcggtta tggtggaatc cgtgattttg gcagctacgg
    10081 tatcgccgat caggttgacc gccttgttca ccgccaggcc cacgaacccg acggatgccg
    10141 aggcgaaggc gctgtcgagc tcctcgtcag cggcggcaat gacggcgacg cccttgacat
    10201 actggacggt ctcctgcaac cggtcgtctt cccagccgct ttcgttggcc ttgcggctcc
    10261 agcgggtcgc tgcttttcct gttccgttgc cgccggccgt gaccgtggag ccgctgatgg
    10321 cggccttgac gttgttgagc agcgtgttca ggacgatggt gccgccgatg ccgacaatgc
    10381 cgcccaggga ggcggtgccg ccgtcggcat cgatatctcc ctcccagtcg gcggcgacca
    10441 ggaccgtgtc tgaagcagcg agggtgcttt cctccaccag cgcttccacg gtattggaaa
    10501 ccgtctggac ggcaatagtg cccgcctccc gaaccgccgg cggcgacggc gtcgatgtcg
    10561 ccctcggccg tggccagcac ggcgatgctg ccgtctgtgg cggtcagggt gctgtccgca
    10621 accttggccc gcaccgtgtc ggcgatctcg ttgtaggccg cagagccccc gagcccccag
    10681 gccaggcccg cggccacggc gccggcccgg acctcgatct tggccgagtc cgtggcctcg
    10741 atcgtgatgg cgcccgccgt aacggtcgag tcgtcggtca cctccgcctc gacggtattg
    10801 ttggtgctgt tcagggccac accgccggcc agggagtaag cggcaacggc aaaaccggcg
    10861 gcggccgcct cgatggtcga ggtttcggaa gccgtaattt ctaattcctg agctccggca
    10921 tccaggttgc ttctcctcac tgtggcatag acatcatccg cgatgtcgtt ggtcgccacc
    10981 gccgcgccca ccgacgcgat gccgctcacc gcgatcgacc cggccagcga ctcgatggtg
    11041 gagtcgctct gggcgctgat ttcgattccc tgggccgtca ggtcgctgtc ccacacgtcg
    11101 gccctgagcg tgttgtcgat ctcgttcaaa gtgaccgagc cgccggccgc gaatcccgcg
    11161 gccccggccc cgcctgcggc gagcgagcgg atcgcgcttt ccgaggaggc gctgacctgg
    11221 gccgcccccc cggcggtcac ggcggagccg tcggtgatcc gggccttgac gcggttagcg
    11281 atgtcattcg tcgccacggc cgcgtcgatc ccgccgcccc cggcaatgac cacggcgccg
    11341 gcgagggaat cgatcgtcga tgagtccgcg gcatcgaccg tgaagctgcc tgcggccgag
    11401 acggtcgaat gcgagatcac ggcctcgacc gtggcgtcga tgtcgttgac cgagacggag
    11461 cccgcggcgc cggcgatgcc cgcgcccacc ccgctcaccg atacggccga aatctgggcg
    11521 gagagcccct ccggcagggt tgcgggcggc cccgcggtcg cggaaaccgt gatggcgccg
    11581 gccgtggatc tggcccggga gtccgagagg accgcctccg tcgagcttcc gatccggttg
    11641 aacgaaaagg ccccggccgc ggccacggcg ccggcagcgc cggcattgcc cgcgatggtc
    11701 gagatcgcgg agcggtcggt ggccgaaagg gtgatgtcgc ccgtggcccg gacgtcgctt
    11761 tcctttccgc cgttggcgga aagggaggct gtcgtcgtgt tgtcgatgcg attcaccgag
    11821 acggagccgc cgatcgccac gccgcccccg gcagccccgc ccacggccac gttgaggtcg
    11881 atcgcctctt cggcggccag gaccgccacc ttgccggcgg catcgaccgc cgcccccgcg
    11941 atggccgaag agacgctgtt gcggatgtcg ttcaccacga ccgaggcccc gattgcggca
    12001 gctccggccc cggcgagcgt accggcaagg ccggtcacct gggattcgtc cagagccgaa
    12061 acaatgacgt cgccgccggc cgcgatcttg ccgcccgtgg gggtgtcggc gatctgggcg
    12121 gaaacctccg cacgcaggaa gttgagcgtg acggccccgc ccagggcgaa ggcggccgcg
    12181 ccggagccgg ccacggtgac gccggtgatt cccgcctcga tcgacacggg cagttcgatc
    12241 ccgtcgagga cgtcgtctgc gggcagggat tcgggatcgc cgaaggaggc gctcacggtg
    12301 atcgcgccct gtgcgtcgac ggtggaaccg gtgatggcgg cggtcacgcc gctcgtccgc
    12361 gcacccgtgc tcccggtcac cgcgggtttg ctcatcccct cgtcggtttc ggtgtaggtc
    12421 agaacgttcg gatcgtcggg gtcgatggat ccgcccacat agttgtaggc cagggaggcg
    12481 ccgacagccg cgccgcccag cgccgccatg ccggcgaccg agtagaggac cggcgtctcg
    12541 gcggccgtga cgaggacgtc cccgtccgcc tcgatcgcgg agtcggcgat gtgggcatcc
    12601 acggtgttgg cgatggaatt gatcgtgagc gaaccggcga gcgaggccgt ggtgctgaag
    12661 ctcccggcaa ggctcatccc gatcaggacc ggggtcgacg tcgccgaaac ggtcacgtct
    12721 cccgaatttt ccatgccaga agcggcaacg gccgtgactg aggccttctc gatggccgcc
    12781 gtcacgctgt tttcgatccg gttgacgccg accgcggcgc cgatggcgac tccgcccgcg
    12841 cccgcaccga cgttcagggc cccggccagg gaggctatcg tggagttgct tacggcactg
    12901 accgtcacgg cgccccttcc ggccaggacc tcggttattc cggtttcgtt ggcgatccgg
    12961 gcggtcaccg tattggtgat ggcattcgtg gacgaggcgc cgccgccgct gacaccgacc
    13021 aggccggcgg ccacggaagc catgacggaa gaggaaagga tggaggaatc gtccgccgcg
    13081 gtcacactga tcccgccctg cagcgccttg acgtatgccg catcgtcgat aaaggcggaa
    13141 acgtggttgg caatttcatt gaaggccagg gaggcgccga tcgatagggc accgccgatg
    13201 ctgatgctgg ccgccacgga agccgcttcg gcgttggcat tgatactgga tgtgttttgg
    13261 gcattcagaa taatgttgcc cgtatccgcc gtaatcccgt ttgatccgtc gataaaagct
    13321 gccacggcgg aagcgatttt gttttgcgcc cagacgccgg agccgcttcc cgagaagccg
    13381 ccgacgccgc ccgaaaccgc tacggacccg gccagcacga ttgcttcgat ctcctgcgcg
    13441 gcctgcgctt tcagtgtgac gccgccgccg gcatgcagtt cggtctcctg caaataggcc
    13501 tgaacctgag cagagtctgc aactctggcg ccgttttcgt catatccgat gtaattttgg
    13561 gcaacggagg cgccgatggc cactcccaca ccggccgttc cgccgccgac gctcgcggag
    13621 acggaggcga tgagcgcctg gagggtcgag ctgttgaggg cctcgatagt gacattgccg
    13681 gtgctttggg cggcacttcc cgccaggaag gcgtggacgc ccgtgaggat ggcattggtt
    13741 gcctccgctc cagcgccgct cacacccaca ccgaccactc ccgaaccgga agcggcaatc
    13801 gaggcggcaa aggcgcaggc gctgatttga gccgtttcgg cggcgccgat gacgatcggg
    13861 ccggctgtag cctgaagaag agcggcatcg ttcacatacg cgtcaacgga gttggcaatt
    13921 acgttttggg ccgcggagac gccgagcgca atgccggcgc tcatggtccc ggaataggcc
    13981 tgcgcaatgg cgactgcccc ggccagggca cggatgagcg agtcatcccg ggcgttcagg
    14041 gtaatggtat cggcgctgat gtttgcggag gtgtcgataa aggcggcaat atccgttgca
    14101 atccggtttt cggcccagac gcccgatccg ctcaccccga tgccgacggt cccgctggcg
    14161 gccagcgcca ccgagcccgc cagaacgatg gaactgacgc tctgggaaga gatggcggtt
    14221 tgctgaagat cgccggccgc ggaaattgtt gagtccgtta cgtaggcccg cacctgggcc
    14281 ggacggctgt ttcctgccag atcggagccg atcaggttcc aggccagaga cgctccgatc
    14341 gaggcgccga cgcccgcaat tccgccgccg gcaattgccg tggaggcggc aagcaccgca
    14401 gaagagacgg ccgccgtgtc ggcggcgctg accgtaacgt cggcggcgct gacaagatcg
    14461 ctcgactgcg cgtaggcatt gacccgggtg agaacggagt ttgcggaaaa agcccccgcg
    14521 ccgctcacgg ccaccccggc ggtgccgccg taagtggcgg ccagggaggc ggccgcggag
    14581 accgcattga tcgtgccccg ggtgacctcg agggcgttgc catttgcgtg cagcagatag
    14641 gtggcgccgg agccgtccac gaggctccag gactggccct cgaccagggt ggtcagggtc
    14701 agctccgcgg tgaccagttc ccagcgggcg aagttggcgt agttctcgct cccgagattg
    14761 acgtgtaacg gttggacatg cttgtagcgg tagacccggc cttcgatccc gcccccggtg
    14821 tacccctctt cgatccggac cgtgtccccg ttgacgagta ccttgacccc ggcgccggtc
    14881 gagtagaggc tcgccggggt gaccgaatcg gtcacggcca gggtctcgcc ctgggcggca
    14941 aaggcgctcc ggatcttttc gaggatggcc gcatcgccgg cgctgtcggc gctgtcgccg
    15001 gccgcggcgg cggcatcgtc caggtcggag gctgcaagcg aaagacccgt cagatccatg
    15061 tcgaagaggt gctcgccgct ggcagtcgcc gcaacggtta cggcgccggt cgttgccgtg
    15121 gcatccgcat ttttgatgta agcggaaaca tcattggcaa tttcattgta tgccagggaa
    15181 aggccaacgg agacggctac accggcagta ccgccataag ctccggagat ggaggccgcg
    15241 gcggccacgg aattgatggc ggagctgtcg tccgccgtaa cggaaatgct gcccgtatcg
    15301 atgtcgccgc cgctgatata gccgtcgatg tccgtattga tccggttttc ggcaaatacc
    15361 cccgccgcgc tcaccgccac gccggtctgc ccgccgccgg ccaccgcgac ggcggccgtc
    15421 gccactaccg cgtcaatgct ctctgcggca gtggccgcaa tcgtcagcgc cccggttgcg
    15481 gcaatcttgc cgccggctgc ataggcctgc gtcagtgccg gaccttccgc caggtttacc
    15541 tgtttccaga cggtggtatc cgaataatct tcggtttgca gatcgatcca gcacgcttcc
    15601 cagtcgtccc ccaggtaatc ggccttggca agattgacgg tcgccccgga accgatatac
    15661 cggtagaggc tgccggtgat ggcagcaccg gtgtaattct gggcaatgga aacgatatcg
    15721 ccgctggcca gctttctctt accgctggcc gtcgtatatt tggccgtcgc ctgggtgcgg
    15781 ctttctccca ggtattcgta gacgtcgccg gcccgcgctc ccgattcgat cagtacgcag
    15841 tcgccggatt gcagggtttg cccccgtccc aaaccgtctt cggtggaata gtcgtagtcg
    15901 gtatcggtgt ccggagacca gccgacgaag ttacgtgcca ccgaaacgcc gatggccgcc
    15961 gccccgccat tttgcggacc gacgccgaca gccgccgaga tggccgccac ctcggcattg
    16021 acttccgccg tgttgtcggc ggtaactgta atattgccgg tgctcgttac attcgcgccg
    16081 ctcaggtatc cggcaacatc ggtgccgatg tagttggttg ccaccgcccc gccgccgctc
    16141 accgccacgc cggtcgtcgc tcccgccgcc acagagaccg ccgcggctac ggaaaccgcg
    16201 tcaatcgagc cttgggcatc ggccgccacg gtgaggtcgt ctaccttcgt aattgttgaa
    16261 ttttcgattg ccgcccggac cgtatcggca atttcattta cggcaaccga cacacccacg
    16321 gccaccgccc cggaattctg ggaaccggcg gaaaccacca cggagccgcc ggccgcatcg
    16381 gcggtaatgg cagccgcatc ggtggccgta atgccgatac tgccggttgc ggactggagg
    16441 gcgcttccgc ctttgacgat tgcgttggtg gtatttgcga tgctgttgcc ggaaccggcg
    16501 cccgcgccgg cgatagccgc tccgatggag ctgccgcccc cggcggccac gcttcccgaa
    16561 cccgccaggg ttagcgcatc gacatcaatt ttcgaggaag cggcaagatc aatgttctta
    16621 gcggcatgaa tccaggaatt atcaaccagg gcgctgaccg tgtcggcaac gtcgttgagg
    16681 gacaccgacg cgccgaacgc caatgatccg ctgctgccgg aaccgccgcc ggcctgtacg
    16741 gcaatggcca caccacccgc atcggcccgg actttagtcc ggtcggcggc ggtaattccg
    16801 acagaaccgg tctgggcctg agcttcactg ccgtcggtaa tcgacgcttc aatattcgag
    16861 ttaagggtat tgaccgctcc cgaacccgcg cccgccagcg tgccgctcat tccggtcccg
    16921 ccgccggaag atccggacgc ggcaagcccc agagcgaagg cctgaacggt caggtcggat
    16981 gcggcggaaa gtgcgacgtc accgaccgca attgccgggg agtcgtctat cgttgcgaca
    17041 acgctttgtt cgatgtcatt gagcgcggcg gccacaccga ccgaagcggt taccgtgctg
    17101 cccgtagctg ccccggctgt gagggcaaag gcgacaccgc ccgcgtcggc atagattttc
    17161 gattgattgg aagccgtaag cagaatacct cccgaaccgc cggctgtaac ctctctgtct
    17221 gcgctgatcg ccgctttaat atcgaaattg acggcattgc cgcttcccgc gcccgcaccc
    17281 gccagcgtga ttttcgttcc cgctccggcg ccgcccgtct gggcggctcc cgctccgcca
    17341 atggcgaggg cgttgatcgt tgcgttggca accgccgtca gttcgacatc ggcggtggcg
    17401 gtaatatacg aatcttcgat caacgcgtgt acggtgtggg cgctcttttg cccgatctcg
    17461 ttggccgccg ctccggcgcc caccgccacc gctcccgttg tcgcggcgcc tccggtcttc
    17521 ttggcggaaa tagcaatggc gacaccgccc gcgtcggcag aaattgccgt attgtcggtg
    17581 gccgcaagct gaactgcacc ggaggccgtg actctcgagg agtcgatgag cgcttcgatg
    17641 gaggtgtcaa tgacattgag ggccagtgcc ccggtaatgg caaccgcgct gctgtcaccg
    17701 ccggcgcccg ctccgccgat tgccacggag gagatatcgg attcgtccgt cgcattgatg
    17761 atgacgcttc tcggagatga gagattcgca tttttcagcc gggcggcggt ggaacccgaa
    17821 acggtgttga tggccgccga accggtcacg gccccggcca ccccggttcc ggttccggaa
    17881 cctcctgctg tgggcacggt ggcggcgatt gccacaccga ttgccagcga caagatgttt
    17941 ccggtgcgtt cggcattgag cgccacggcc ccggcactat tcatctgagc gccggtaatc
    18001 agggcttccg tggtcatttc aaggccgctg tagctgaagg cgccggcgat accgactgcc
    18061 gttttggatg ttttggcaaa agcggcggca ccggccaggg tctgcacggc cgtgctgtcg
    18121 gttgccgcaa gttcgattgc cccgtagttt aaatccaggg aggcgctgtc gttgatatag
    18181 gcccgcgtaa gatcgtccac ttcggcaatc gaaaccacgc ccgcaatccc cgcgccgatg
    18241 gctgccggct gggcgccggc gccggttgta tctttaatcg tcaaaccggg aacatccata
    18301 acggccgcgc cggccacacc gatcaccaga atatcgccgc tgtttacggc actcagggag
    18361 agcgcggcaa catcgagaac agtgcccgca tttccgaccg ccgtgtcggt ggcagtcagc
    18421 cggttgccga tcagcgcttc ggtgttgcgc tccagcagcg tcgttccgga gctgacgccg
    18481 attccgagct gccgggccag ttgtaccgcg ccggtaaagt tgaggtgtgt gacactgctt
    18541 tcggcatcga ccgccagatc gccgccggtt attttggccc cgctggcaat ctgcgccacc
    18601 gtatcgcttg tctgctcgac cagcgcgaag ctgccggaaa ccccggcttt cccggaattg
    18661 ccgccggtct ggacatagtc aaaggtaaag atatcttccg cggcgctgac cgtaactgca
    18721 ccgttcgctc ccgcatgcac ggcagaggct ccgatttggg cgatggtttc gttatcgatc
    18781 gactgcacca gcaccgagcc gccgattccc aaggtcttgg cttcgttgcc gaacagcgag
    18841 atgggcgagc tgcggccccc gatcttgcgc gccttggtga ggttctggat cccctggtcg
    18901 ttgagcttga gctggaccac cccggcgatg ttgatcaatt ccatttccgt ggtcgccgcc
    18961 accagcaccg actggtccgc gttccaatag gtattgtcct ggttgatcag agccccggcc
    19021 tcgatcaggg cttcggagtg gttctggtag aaggtcatcc ccacggaccc ggtgatggtc
    19081 aactgggaat cgggagcgcg gcccgtggtg gtgacccaca tgttgaagac gcccgtcatg
    19141 ccgagctggc cgtcgagcaa cccggccacc tggtcgatga tgctgtcctg ggcgatgtca
    19201 aagccggcgg ggttgaaggg gatggagtcg accaaaagcg ggtaggagag gtccgagagg
    19261 accgagaccg tttgcctggc atcgatggcc gccccgccct tgacgatcgt ctgcacgttg
    19321 ctgtcgtagg cccccacgcc gatcgccagg gagactgcgg cgaccttctt gccgccgcct
    19381 cccgtcccct tcgatttctt ggaattcgtc gcctcggtgg agatgttccc ctgggcgttg
    19441 acggaggccc cctcttccgc gctggccgag atggtgacat cggcgccgga tttgagcacg
    19501 gccgtgggac ccaccgtggc cgtcacgtcg tggtcgaaga ccgaaacccc gaccgaggcg
    19561 gccacgctca ccgccgtctt cttgtcggcg ttgtaggact tgccggcccc cttcaccacc
    19621 gatttgagca ccttgggatc gggtggcacg ttcccgccga tgatgtcgct cagactgggg
    19681 atggcgccca cgctcgactt gaccacggcg ctgttggaag cctcgaggct cgcaacgacg
    19741 ttgatccccg ccttcttatt gggggcctgg agggtgtggg tcagcgtcgc agcgccggcc
    19801 tgctgctccg cgctcagggt gaggtcgacc ggcgccgcag ccagggccgt caggagggac
    19861 tccgaaagcc ggatcgtgtg gctgtcgacc acggtgacga agtagatttc gcccgcggtg
    19921 aggcccccga tggccacgtc gtggcgggtc ccggtggagg cgcgctcgaa gccgtggacc
    19981 tctccggtgg ccaccccccc gacgagggta accgccgcga ggctcccggg gttcgcggga
    20041 tcgttggccg gattggacgc atcggcgagg ccgaagcggt tcgcgtccag cacgatcaca
    20101 tagtaggcgt ggccgtcttg aagccccccg atcgccgtgc cgccgccggc gaggtacgtg
    20161 acgagctcgc cggtttcgta tccgtggttc ggattccaga tgccgccctc gacgacggcg
    20221 ggcttcaggg tcgggtcgaa atagagcgtg gcgatctcgt tgccgtcggc gtcccaggtg
    20281 gtgagggcgt ggaggtcgat gccgatggcg cctatggagg tgagcgcgac cgccttgccg
    20341 ttggctgcgt cgtcggccga cgcggccagc cggatatggt tcgcatcgac ggtgatgatg
    20401 tagtaatcgg ccccttcggt gagccccccg atcacctcgc cgtcgctggc cgaataggtg
    20461 acgagctggc ccgcggaaag gccgtgggcc gcgatctcga tcgtggcggc ggcaaggtcg
    20521 agcgaggaaa ccgtgcgcga cccgtcgaag gtgcagacca cggtggaggc cgagaacccc
    20581 tgcatgcctg ccgtgtggac cgaggtgaag tcgatggcga tcccggccga ggcgttttgc
    20641 tgcgaggcgg cgagctgcaa aatgtctccc tccccgcgga tcgcgtagta ggtctggccg
    20701 ctcacgagcc cgccgatcgc cgtcccgccc tcgccgaccg catagacgat ctcctgcccg
    20761 gtcgcgaact cctggttggt gatcaccaag aggttgtcgg cgatgtccac cgcgttggcc
    20821 gtgagggtat ggccactccc cgtcgcgccc gcggccgaaa gatccgtgat cgcgatgccg
    20881 gccagggcgt cggcgcgcgt ggcggcgagc tgaatgcggt cgtcgccgct ggcgatgacg
    20941 aagtagtccg tgtcgccggt caggcccgtg atcgccgtcc cgccacccgt gtcgtagtgg
    21001 atccgctccc cggtgacgaa atcgtggatc agcccgagca cgatggtcga accgtcggcg
    21061 tcgaccaccg cctcttcatc gatgatgccg gagggattga aggcgacggt gctgtccgca
    21121 acagaaaact gggttccccg gtcgatgtcg acctcggtgg cgaagctcgg gtccgtggcg
    21181 tagacgaccg cgtccccggt ggaaaggccg tggttatagc cgaagtcgat ggcgccgttc
    21241 tcggtgtcca ccgcatctac caccttgaag gtcagcgcgg tctggacata gctcagcagg
    21301 tgggtgtggt atcctgtgaa gctgtgggcc gtgcccgagg ccctggtccc gtcgagggcg
    21361 atggccgtgc ctgcatcggc ggcctccgcg gacagggcca gttggatccg gtcggtgccg
    21421 gtgacgatgg cccaataggt gccgccgtcc acgaggccac cgatggccgc gccgccgccc
    21481 gtcgcgtaga cgaggcgctg gcccgtggta aacgcgtggg tgtcggcaaa gacgatctcg
    21541 ttgcccgaaa ccgcggtggc gccggtgtcg gcgtccgcgg tcgtgggatc gaaggtgatg
    21601 gtgctcttgg tggcgctccc ctcggtcagg atcaggtcga tgacataata ggtcgtgccg
    21661 ttgcaggtga aggtttcgcc cgtcacatcc tcgtcttccg gattggcggt ggcgagcttg
    21721 atcgtgtcgg cgtcgacctt ggccaccacg taggtgccct cgttctccaa gccaccgatc
    21781 gcctcgccgg cgtcggaggc cacgtagtaa tcgaccacct ggccggtggt gaactcgtgc
    21841 gccgtgccga aggtgatcgt gttttgtgtg acgtcgacgg ccgtcgccgg ctcgaaggag
    21901 atcgtggcct tgcggctgac gctctggacg gagtcggggt tgacgttgag cgtggcggtc
    21961 gtgtcgaatt cgatggtctg gccgcgcacg agctggatag tgttttcgtc cacgaccacc
    22021 acccggtagg tcgtgcccga ctcgagcccg ccgatcgagg aaacggtgat tcccgaatcc
    22081 ggatctgccg cgaggtagag gagctcgtct cctgtcttga ggccgtggtc ggggatggtg
    22141 atcgtgtcgt ttgccgtgtt gacgttcgcc agttccaggg agatggccgt gctctcgccg
    22201 gcggcggtga ccttgccgtt gatctcggcc ttgatgtcgg tatcgtcgat cgccagggcc
    22261 agccccacgc cgccgaagcc gtccacgaag atcgagacgc ctgaatcggc gctgttttcc
    22321 acctgcccca ccgcggcgat gttcgcgttg ccgccggcgg taatgacgac ggagctgtcc
    22381 actgtggtca gcgacgtggt ggaggcgttg gtgaccgcga tcgaggtggc gcccacggag
    22441 gtattcgtct tcgtcttttt cccgcccggc gcggagttgg ccgcggtgta ggactgggcc
    22501 gagacgccgt tttccgcgtc cgagccgacc gtgaccgtgc cgcctgccgc gatggtggta
    22561 cagcccgaga gaaccgtctc ggcgaccgca tcggcctcgg cgtagctcac ggcatagggg
    22621 gccgagttcg caagctgcga ggcggtgctg ttccctctct tgggcttggt caggtcgatc
    22681 ccgccgccgg cggaggccgc gctctccacg ctcgtactgg tatggatcgt gacgtttccg
    22741 gtgctttgga tggaggcgcc gtcgagcgtg acataggact ccgcggtccg gacggagacg
    22801 gagaccgcct ggagcgcggc cccggcgatc ggaatcgacc ccatcagcaa accgatgacg
    22861 tcgccgtagt tgagcagcgg ggtgagcagg tagttgctga agatgttccc gcccgggctg
    22921 tcaaacaggc tttggtcctc ggccgaggcc tcgatgaaga cctcgctgcc ggagatgacg
    22981 gcgttcttca agtcgatcgt ggcggtcttg gactgcaccg agacgatcgt catgggcgat
    23041 ccggtcatgc tcgtgcggtt cgccgtgatc tcgatcgcgc cgccgcggag ctcggcggcc
    23101 cccacgcgga cgaccacgtc atcggcgggg aagacgtcga cgggcgagag ccccgaaagg
    23161 tcggtcacct gggcggtcag ggtaatgtcc cccggggcgc cgccggcctg ccccacggtg
    23221 atgagcctgg cgttgtcctt gacgtccacg gtcgatccgg aaaacgcgat gtcgcccgcg
    23281 ctttgacccg cggccccggt cgtcacgagc gcgacgccgg cctgcacggt gacggtctcg
    23341 gcattggcct cgatgtcacg gccgtcgaat tcgttcgacg ccgcaaaaac gatgctgtcc
    23401 tcgccgtcga cccagccgga atcggtcccg tagccgccgt tgatggtgag gtcgtagccg
    23461 atttctccca gatccccgac cgtgaactcg tcgttcccca ggcccatctg gatcgtgagc
    23521 gacgaggtgg gggcagcgaa gatgaactcg ttctcgtcat tgtagagccg gagcttcccc
    23581 aattcgagtc ccgcagcgag ggtcacctcg tccggcccca aggtgagcac catcttgagg
    23641 tccgcgtcgt cgcccacgag gaagcccatg gtgaagaagt ccttgatctt ttccgacacg
    23701 ccgccggaat aggaaaacaa attctccccc tggccataga ggatcttctc gatgccggag
    23761 acgccgctta acgtattggt cccgtcggtc accgagagcg tgccgtccgt cgcgaccgca
    23821 aaggtcagat ccgcctccac attggagaag tccaggatgt ccgtgccgga gctccccgga
    23881 tcggacatcg tatccgtccc ccacccgtcg tcgaagacat accggtcgcc ctcgtcagtt
    23941 ccggtcagcg tttcgtcggc gtcggtgccg acaatgtctt gcagcagata accgtaagcg
    24001 aaatccgcgc cggtgaaaac cgtcccggtt tccacagttc ccacgacata ttccagctcc
    24061 cagtttccgc cgtccgccga tccggtggtg tccgtggatg cagccacgtc cgcgccggtt
    24121 atcccggcca gggtctcgac gaattcgatt cccgcggcgc ccgcggcgac atcgcaaccg
    24181 taaaggagaa tgtcgccatc atctttcagg gagtctgccc aggcggaaat ttccgcctcc
    24241 cggctttgga gcacctcctg gagcaccgcg gtcgatccca ggctcatttc gcctgtgccg
    24301 ccgtgggaga gaatgtgcaa ggcgtcgaga tcctgatagg acttcagaat ttccgagatt
    24361 tgttcgatgc cgtccgtgtt tgccgtaagg acgcagatgg tccattctat ctccgtggac
    24421 tgcgtgtttt cggcggcaat tttcgccttg agttcggtaa cgagggattc atagtcgtgg
    24481 acggaagaat cgacaataac cagttcgcgt aaaatccgct tttctccgga aagcgcggaa
    24541 taatcggaaa ccgcttcctc cgctgcggat tgcgccgccg gcaatggcgt agaaatgtcc
    24601 gcttttgatt tctcttgcgc cggattggcg ccgggaatct tgccgggtga aaatgccggg
    24661 ggttgccggt ccgcctgatc actgccgggc acggctgccg tcagtggctg atcaatattt
    24721 tccgcatttt ccgcaggcgg cccattttgc cggatggcgg ggttgagttc atcggctgct
    24781 tgcaccgtat ccgcgggggt aactgttacg ctttcgacaa cagccggaac aattccgggc
    24841 gcttctttga tgtcgcctac cggcagagga acgtctgcgg agaggaggag tctcggctcc
    24901 agaggttcca gctggaacgg gcgccggtct ttccggcggg ctttcttttc cgttttttcc
    24961 gcggatgttt ttttacgccc caaactccac atcaaaagcc tcttttgcta tttactgtct
    25021 tccagccacc cctgcacggt aaccatgtcg ccttccgcca atgttccggc cgcctttttc
    25081 aggcgcagac tattcatgac ataatcatag cgggccgtcg cataatcctg ccggccccga
    25141 gtgaggtccc gttgcgcatc cagaaccgcc accaggatat acaacccgga gcgatagccg
    25201 tcctgcttgg cctgcacgag aagttcctgc gcttccacgg cctcgcgcag cgcggcaacg
    25261 cgttccatcg catcaagcac gccgtgatag gcggcccgtg tttccttttg cagcgcccgc
    25321 agttggcgtt cctgttcctg gagggacgcc tggtaaagat tgtaagcttc ccgcgaccgg
    25381 gaattgacga cgccgccctc gaaaatcggg aaattcagcc gcaccaggac attgctcgtt
    25441 cccgcttcgt agccgccgcc gaagagtgtt ccttgggtat cgatattaga atacttcgct
    25501 tccagattca acacagggta atgcccggca acctggcgct tgtactcctg caaagccatc
    25561 tccacgcttt gccgctgaac ttcaatggcg ggattctgct tgacggccgc agcaatccag
    25621 gcatcggcct ctcccggctc cggacgcttc aggggcaagt cgctacgcaa cggcgccagc
    25681 gacgcggctt tttttcccgt aacttcgcgc aaggcctgga gagctccgtc cagagcgctt
    25741 tccgcggcaa tccggttcgc ccggacttcc gccagccgcg ctttggcatc aaggtaatcc
    25801 gtcttgcggg ccaggcccag cgaatattta ctgtaggcca gctcatattg catccttatc
    25861 gacgcttcct cggtttgcgc caagttaagt ttgtcacggg cggccagaac tcccagatat
    25921 actttggtga cgcgcacaat gagatcctgc cttgctgctt ccagttccat atctgccccc
    25981 ttcaccttcg cctgcgcccg gaagagattg gacagagagc tcttgttgaa cagcggctgg
    26041 atcagcgaca cggaatattc cgtagtcgga tacgaggatg atcccggggt ataggaggtg
    26101 ttgtcacttc tgccgacgtt ctggtgggtg ccggtgtagg ctccctcggc ggaaattttc
    26161 ggcagaaacg cggaacgtgc ctgcgccagt gtctccttgg aagcatcatg gctgaactgt
    26221 gcgccctgat aagccggatc gtttttgatc gcctgttgat aaatatccag aataccttcg
    26281 gcctgcgccg ccgacagcgg aacaagaaac aaaacggcca ggagtgtgac aagttctctc
    26341 cagcagaaag cggacggctt tcccatactt ttataatggt tcgatatcat catgatccgc
    26401 aaccatttca tttttaacat gaatcttaaa ttgagaaaat ccgaccagca gtcaggaaaa
    26461 gacaaaaatc ctgcatgcaa tattcggatt catatttgac ggcatcgtaa aaagcgccct
    26521 agcccgccga ctgccgggcc tgtataaaag aagtaag
//
LOCUS       DAOPOE010000044        25604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2445_length_25604_cov_5.855298, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000044 DAOPOE010000000
VERSION     DAOPOE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25604
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_25604_cov_5.855298"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PKM94_07425"
     CDS             complement(<1..1403)
                     /locus_tag="PKM94_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOD66510.1"
                     /translation="MANPLFAKKPLALCLEEMKGENRLRRILGPVRLTSLGVGAIIGT
                     GIFVLVGQVAHDKAGPALMLSFMVAGLACVFAALCYAEFASMVPIAGSAYTYAYATLG
                     ELPAWIIGWDLVLEYTVSSATVAHGWSKYFQDFLGIFGLKLPYALSTAPFDYDPGVGH
                     LVSTGAVIDLPAVVVACLITFVLVKGIRESAGFNAALVAVKVAIVLFVIAVGAFFINP
                     DHWRPFAPYGYTGISFFGNLVSGQADAGGAPLGMLSGAAMIFFAYIGFDSVSTHAEEA
                     KRPNRDVPIGIIASLIICTVLYLAVAAVLTGMVPYDRINIEAPVSDAFRQIGLPWAQF
                     LISLGAVAGITSVLLVMMLSQPRVMLAMARDGMVPPSFFGAVHPKFRTPWKSTILTGI
                     FVSVMAAALPLRILAELVNIGTLLAFVIVCAAVLIMRYTNPHAERPFRAPLFPLVPLL
                     GIGSCVLLMFSLPVENWL"
     gene            complement(1560..2297)
                     /locus_tag="PKM94_07430"
     CDS             complement(1560..2297)
                     /locus_tag="PKM94_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66511.1"
                     /translation="MMPCFRKGRLGAAMAVLAAPAVLLLAPAAAAQPPPTPPRAQAEP
                     PAPAYPEQFFLHAGVGGGLAVFSSGDIERRLSDEFHESFKLTNAGVDFAYLVRCGYRN
                     IAQAEYRVEKGFGHKFYYDLGTASTASTDRVEVEANVDSWEWLLKINPAFTWLRDKSM
                     GIYLLYGRGTSEYFGDLGVQFDGGEKELFGIEFLKLYRWYAFTVSAEYHTITYDSYAD
                     EDSRPTAGAWDASYWIIQGSLSAGFGD"
     gene            2472..3320
                     /locus_tag="PKM94_07435"
     CDS             2472..3320
                     /locus_tag="PKM94_07435"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOD66512.1"
                     /translation="MDGVKKIVIVSDGTGRTAKRLMDAVLSQYAQAEVGFRLEGTFQN
                     VRNRKAVDKVLAEISNEYLVIYSFISPDIHKYFHRRLAERDILHINVLDPMLKTMRKF
                     LGVHPDYRPGILHRIDDRYYKKVDAIGFTVEHDDGRGAMIGDADLVLVGLSRTCKTPI
                     SMYLACNHGLKVANIPIVPGVTTELALKHHLGHLPRERIVGLLMDPDILSHVREERLE
                     LLARTDSAVAELSDYVDVRVIRRDYRWCRELFERNGWRTVDVTRRAIEEVAVEILDEL
                     GIPREE"
     gene            complement(3328..4878)
                     /locus_tag="PKM94_07440"
     CDS             complement(3328..4878)
                     /locus_tag="PKM94_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD66513.1"
                     /translation="MKLVTAEQMRAIDRETIEVHGIPGPVLMENAGRGIAERLAVAVI
                     PKVRGAKVAVVCGKGNNGGDGFVVARHLAGMGAQVAVYYLGPPEQMSEDCRLNFERAK
                     ELGFPMTEVTAVEALPETLPDDFIVDAVFGTGFSGAPRGLAGELIEYINRHEDRTVIA
                     VDMPSGLNADTGEGEGAVVQAHYTFPLALPKYGLYVSPGRELAGAVAVVPIGVPDEVL
                     AKFDLRSDLITLGMVRQWLPDRPPDGHKGTFGRLLIVAGSLGMTGAAALAGTAAYRCG
                     CGLVKIGCPRSVLPIIAALIAEATSHPLPDVAKRGALALRGLGEVRRLAEDHDAVAVG
                     PGIGRHRETFELVRRFTAGLTKPAVIDADGINAFAGHAALLAGREGGAALVLTPHPGE
                     FARLTGETVPAGIHDRMALVRKWAKELRAVLVLKGSPTLVAAPNGSCFLNPTGNEGMA
                     CGGTGDVLTGAIGSFLAQGMGPLSAAVCGVYVHGMAGDFAADARTSRAMIAGDLNQYL
                     SEVFGQLE"
     gene            complement(4875..7289)
                     /gene="lon"
                     /locus_tag="PKM94_07445"
     CDS             complement(4875..7289)
                     /gene="lon"
                     /locus_tag="PKM94_07445"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOD66514.1"
                     /translation="MTTDQPQTPDEAKQAAGPRILPILPLKGTIVYPYLVVPLMIQDA
                     EQARLVDEALMRGSRIGLFLQKDAEQEHPGPDDLYHTGCSGSILKMLRFPDGTVRFLI
                     QGLARLRIVRFTHTERYLMAEVEELREQIDDGIKIDALQRSILGRVQQLVELAPYLTE
                     EFHVSAINQDSPSKLADFIASNLNIPIRQKQEFLEETEVLRRLQKLHQAINKEVEVLQ
                     LSQKIQAQAASELGKSQRDYILREQMKAIQRELGQGEETSEIEEFERKIAEAHMPEVA
                     AQAARKEVDRLRHMNPSSAEYTVSRTYLDWLVSMPWSKSTEDRLDLRRAKKVLDDDHY
                     DLATVKDRILEYLAVRKLKADIKGPILCFVGPPGVGKTSLGRSIARAIGREFMRVSLG
                     GMRDEAEIRGHRRTYIGAMPGRIIQSIRRAGSNNPVIMLDEIDKLGADFRGDPSSALL
                     EVLDPEQNDTFSDHYLEVPFNLSRVMFITTANWLEPIPAVLRDRMEVIRIPGYTDIEK
                     LQIARRHLIPKQLENHGLTGDQLVVEDSAVTAIIEGYTREAGLRNLEREIAAVARKAA
                     RKVAQGDSAKVVVTGKEIPKLLGPQRFTREVLMRKGQIGVVPGLAYTSVGGELLFIEA
                     TSMVGKESFTLTGQLGEVMKESAQAALSYVRSNAETLGIDPTVFEKRALHIHVPAGAT
                     PKDGPSAGITMAVALASLFAARPVKPCLAMTGEITLRGQLMPIGGLKEKLLAAYRAGI
                     EEVILPEDNRKDIAEIPPEIKKAIRFKYFSAAIEAIKYALDRPAVCGGAAANAAAGRK
                     RKRPGR"
     gene            complement(7399..8451)
                     /locus_tag="PKM94_07450"
     CDS             complement(7399..8451)
                     /locus_tag="PKM94_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOD66515.1"
                     /translation="MKLGIIGKPQAGKTTIFNAASGQQEAVGDFSQAAHRAIIKVPDP
                     RLAVLAEIEDPKKITPAEIEFLDAPGFTGKGKEAGAVEISQEVRLMEALILVVDAFSP
                     DAAPERDLRNLTEEMILSDLSIVEGNLDKKGRKMKLTGDKSATHDLELLETCRAQLEG
                     EQPLIDLDMAEEDLKALRGYSFLTLKPLLIAVNIAEDALDRAGAMREGYAGLAAPGKR
                     DVAVVCGRIEAELAALPEIERAAFLADLGIATPAVDQVIRKSYDLLGLISFLTAAKPE
                     VRAWTIRAGTTAQRAAGVIHSDIERGFIRAEVTRFEDYARYRTPAALKAAGKTRLEGK
                     DYVVQDGDVILFRFNV"
     gene            complement(8772..9623)
                     /locus_tag="PKM94_07455"
     CDS             complement(8772..9623)
                     /locus_tag="PKM94_07455"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOD66516.1"
                     /translation="MQFGAHESIAGGVFTAIERGHRATCDTIQMFNKSNNQWKAKKLT
                     TEEIDLFFARIEETGVTAAVSHSSYLINIASPDEALAEKSYASLKEEMERCRTLRIPA
                     LVLHPGSHVGSGEEIGMQRVALSINRLFDELPDNTVTLLLETTAGQGSNLGCTFEQLA
                     YMIDRVDNKAQIGVCLDTCHIFAAGYPIVEPADYRKTMKQFDEVIGWERLKVIHANDS
                     LKELGSRRDRHEHIGRGHIGLKGFGNFINDRRLKKIPMIIETPKGDDLTEDIENLKVL
                     RGLVKKK"
     gene            10086..11108
                     /locus_tag="PKM94_07460"
     CDS             10086..11108
                     /locus_tag="PKM94_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66517.1"
                     /translation="MCTFLSVDEAAFTDTHWRQFHSFLVNMHARGFGNVPPSTAQALR
                     QRIEARLALRDGFREWVIVKDSAVVGWLGIHVRPGSETAPPVMFLVFDFVPGLEVAPL
                     SARAAGLVAAAMRENRCGEVHCMAAEAHLDSLRQTWGGRILSRLHRYRLDRRTANRDL
                     INEWLAEFPRQFPSLRIEIFTEIPERHLDSWIALFGQFLADMPNEGTAAAPFTMTRAE
                     VRKFEALRRRNGQYLYTGALLDGEGRMIGHSNMATHDDPPTEWHQAMTGIERAWRGRG
                     LSKWLKAALFVRTGEDFPQSRYIWTEMRAVNEPIHLVNRAMGFELSSEGYEWSIDRAA
                     LDRLAG"
     gene            complement(11172..11531)
                     /locus_tag="PKM94_07465"
     CDS             complement(11172..11531)
                     /locus_tag="PKM94_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD66518.1"
                     /translation="MKFRVIKHHQAAGGPSRELESGERLQFERRPSEYEGWVWCTAED
                     GGGGWVPETWLTITQDPAGGATAGWCFLKRDYSPEELTVHPGDILEGRREESGWLWAC
                     TADGRKGWVPRDAVEPV"
     gene            complement(11610..12020)
                     /locus_tag="PKM94_07470"
     CDS             complement(11610..12020)
                     /locus_tag="PKM94_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOD66519.1"
                     /translation="MTPRSDNPARRRVSSGSPYEEPFGFCRAIRVGNAIHVAGTGPIA
                     ADGSTGAPGDPYGQTMRCLEIIRTAIEQLGGRAADVVRTRIYVTDLTRQDEIGRAHGE
                     FFREINPAATMIGVAGLVRADWYVEIEAEAIVDE"
     gene            complement(12025..13455)
                     /locus_tag="PKM94_07475"
     CDS             complement(12025..13455)
                     /locus_tag="PKM94_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOD66520.1"
                     /translation="MARTYKMYLGGEWVDRADKIAVANPFNDEIVGAAASASAEDYTQ
                     AIEIAHRTFAVTRDLPAYKRSETCLAVANGLEKNIEQFTKMMCRELGKAYRDSRAEVK
                     RAIGVFTVAAEEARRIGGEIIDLDWAAGSEERVGLVRRFPRGVVAGIAPFNFPLNLVA
                     HKIAPAIAAGNTIVLKPASKTPLIALMLAALIDKTDHPKGAVSILPGSSAAAAPLLED
                     RRVRLITFTGSSAVGWWIKKHAAEKEVVLELGGNAGVAVADDADLAYAVERLAYGAFA
                     VAGQSCISVQRIFVHEDVYDEFVRRLKARVKRITVGDPLDPNVDMGMMVDRASVQATL
                     DTLRRAVAQGAKVIAGGKALCDGRTLEPTLLENVKADMDVCSKEAFAPLAVLMKYRDF
                     HRVVDAINDSDYGLQAGIFTQRIKDIWYAFERIEAGGVVVNDIPTYRADHQPYGGMKS
                     SGVGREGVRYAIQDQTEVKILSLNLK"
     gene            13793..14158
                     /locus_tag="PKM94_07480"
     CDS             13793..14158
                     /locus_tag="PKM94_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD66521.1"
                     /translation="MPMSRCRDQRGLTLLEVLVAMVVISASLLLLLGMGVTALHGNHW
                     ANKTTVATQMMQQRLEHIQSTGDFSNGTASAEGVDVSWTCTPIGNYLRRVDISANWVD
                     AGTRSRTNSLTAYITSDSL"
     gene            14170..15006
                     /locus_tag="PKM94_07485"
     CDS             14170..15006
                     /locus_tag="PKM94_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66522.1"
                     /translation="MAQTREHTSGARGTSLLEVLIALVVMGVITTALFGLYITQHRTY
                     ISQDDVTLVQQNARASIDELARHIRMTGHGLPLEMMPLSAFNTDPDTIVIRYRTDNCE
                     TYLAEAMATPSSDLTCATDVGCFQPGEWVYILEPDSGGGEWLALSEVQTGARVLRHGT
                     MVLSKAYAKDALVLTARQVTFYVDTTTDPQHPALTVKVPGQTPQVFAENVEDLQLQYR
                     MKNGLVVDVPVVVSDVREVLISITARSNRPDHEMAEVTGHSDAYRRRTFTTSVFLRNV
                     GI"
     gene            15023..16669
                     /locus_tag="PKM94_07490"
     CDS             15023..16669
                     /locus_tag="PKM94_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HOD66523.1"
                     /translation="MTTRLPTTAGHRGFATFIALILVVMLTLLGIAALSTSDDEMKIA
                     GNELHEMKAFYAAEAGLADAAAAMRYEYDSTGLPPAVLPSGTSNLNNCLVTFTTADDG
                     AASPRILTSGTLAGLHALVKSFTITSEAENLQDGAKVTLSQSFEVALVPLFQFAVFYD
                     NDLEIAPGPEMNLIGRVHSNGNMYLQSNNTLRMNSYVTAAGDIRHGRKGPGSAGYGDV
                     QIKSASGAFVSMKQGSGWLDAGDPQWYDSSVGRWQGRVQDAAHGQESLNLPLSQEAGG
                     DPHRLIERAAGNVDSYENKATLKFIDGRAFQRVGGIWTDITADMTSRNIISSSDDKFY
                     DQREKKWVDPMELDLGKLYDEGYGPANGVIYFSDQTSDFPALRIKNADELDGGLSVVS
                     ENPMYVVGNYNATDKKPAALIADAITFLSSAWDDSKSTLSKTQRVATTTVVNACYATG
                     NVETTSSDYSGGFENLPRFLETWNGKTLRWKGSAVNLWASRQATGTWNGTYYDPPNRD
                     WLYDTDLDDPYKLPPETPMIRVFQRSGWKQEHVGYADADF"
     gene            complement(16730..19147)
                     /locus_tag="PKM94_07495"
     CDS             complement(16730..19147)
                     /locus_tag="PKM94_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOD66524.1"
                     /translation="MRFHRWTALLAAAAAIPLGAKAQEVPFPDDVFYYAPAATAFGPE
                     AVWVNPAVLGPYKPATVAFMGDLAEGSVLKNWGTVITRRGFGLAYRRIDSDAGEDFKE
                     YALAGGLSLGSSLSVGGSYRYFPSGPSPYDHRHYWNIGLTSRGNSPFAVGAVWSNLNR
                     SELEGEDTRVIQRYSLGYRPQGATVTLAVDALSSAAAAEDDWTYIYHAQVIPATGLSI
                     EAFVDSDGNFEIGARANLLEYFVGTQSHFDSDAGHRFSHLVVGATDSRQPSLIRGSAR
                     RLSVSVSGAADENPVQTFFGARRPAFADLVLGIYRAAGDPGVGEMVLHLDNLRLTLAQ
                     AQEMREAMAWFRAQGKSIVSHLTGPNNIAYYVACAGDSILIPPVSDLDLIGLRAELTF
                     FAGTMEKLGVAADMVRIGDYKTAPEAYTMRTPSEANREMTNRLLDDLYAQFVGGLAEG
                     RGMDPEAMRGLIDSGPYTSTEALDLGLVDGLTYRDRLVPDKFLSPLPEISFRAYRADT
                     LMNDGWPPLPEIAVVYADGEIQPEGAADPSPLSGRGREVTPALMESAFGQALANPQVQ
                     GVVFRIDSPGGYALAGNEIFHRAELAARQKPVVVSMGSRAASGGYYIAMAGRHIFADP
                     ATITGSIGIFGGKPVLAGLYDKIELGKELFTRGRHAGMMTSMRPFTDEERARLGAMLG
                     AFYDHFVGLVAENRRLPRDSIDQLGRGRVWTGQAGREHGLVDELGGLKQALDYTASHL
                     GLREYRVVRYPERRRLIDLPQVPVLGWLGRWAGLVGEEVRAAAAPLADLPEGELLARL
                     PYDIDIR"
     gene            complement(19147..20226)
                     /locus_tag="PKM94_07500"
     CDS             complement(19147..20226)
                     /locus_tag="PKM94_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66525.1"
                     /translation="MTRSHSRPARRAACALLAAAVVGIGPARAQEIHDAGEGRFATRP
                     LTVAAPLAPGEKLLVRSAATLAGKITIRATETDRAVVVYTKQAVTDSRSRAVDYIDLI
                     AVDIGRVADGARLQLRAPNPAPWSGAESGSVDAEIRVPENVFVDIDALYFDVTAEGPF
                     SGVVNPSSLGRLEVYRVRGPVDLGTANRRVILEDVAGDIAVRTSNSTLTASDIESREH
                     QAVFRNDGGEIRIEGLTGEVSIRNDFGLIDVTDFRPLGRRNVIRGQSAPITLEIADLG
                     ESQLVVSNRFEDVDITAAADLSAVMSLAVGESGRIEVSGLRFQTDLVQRDRLGLVVGG
                     GQGFISSSISGSGNIYIRGRGQGEE"
     gene            complement(20210..20998)
                     /locus_tag="PKM94_07505"
     CDS             complement(20210..20998)
                     /locus_tag="PKM94_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD66526.1"
                     /translation="MQVTGTELKNLQALLTKKGRRRQGRFLAEGIRLLEESLRHSFRP
                     DEVFYAPADLSARGRELAARFRAARVPCRELSVRQLSKLADTPAPQGIVGVFTIPRID
                     PAELCARRHRTILVCENVADPGNLGTLLRSALAFGFRLVVTVGTTAEPFAPKVVRSSA
                     GAVFGVRIAAADCDTLARLVASEGITLLAAGRGGTQTLSESLRPDPAARYALAVGSEA
                     EGLSDRMRALAACTVRVAHAPEVESLNAAVAGSILMKGIYDSLS"
     gene            21064..23679
                     /locus_tag="PKM94_07510"
     CDS             21064..23679
                     /locus_tag="PKM94_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD66527.1"
                     /translation="MKTPALTLLCSLLLVSLLHAENRPIIIGGLDGLDPWAEEAGYPV
                     LLALSGGGIRGLAGIGALRAFEETGIRVVAIAGTSMGGVVGGLYAAGYSPAELEEIVH
                     GLDFAALFQNTPPRATMLQTRRREHGRDLIRLRFEGLRPQIPQALTSAQYLTNVLTEL
                     TTQATYRSDGDFSRLRVPYAAVSTDIISGQQEVLRSGSLADAMRATMAFPLAFTGLEI
                     DGKFLMDGGMVDPVPVDVVRDLSDSTDFVVAVNTASTLRSREELVTPLDIADQATTIM
                     TAQRLRESLDEANFVLEPAPPGIQSTDYRFSDSLIALGYRAALAAADSLRRLLEQRVD
                     HTEFTVASVAAATFSPEAQAIFRDALLNRPFTRAGLIRELKRLVRALDLFRLEAVLEP
                     ATGGGAPGEAVELRLNGFANFAPGAAAFLFFGNTRFDDSDMEETLALGDSAVTPASLR
                     RGLDRILGRYAAAGWDLACIRRTSVDPDRARITIVIDEALVRDIAVDGVRRTRPWYVR
                     AHCPLRSGEPYSTAQAAAGLKNIYGTDLFDRVTIDIRPSGGRPVVVIGVEEKAYPQVR
                     LGWHWDDEYESEEFIELADDNLGGIGVEGLVHARYSPDRQDYHVGLRADRIFKTYLTS
                     TISGYRTLLDRHLYDADDSLIGARRESKAGIEIRLGQQIARLGTVTAGLVVEQVEYSH
                     EPDGSVESDFGLRILEIESMIETLDRVPFPRTGERIYLQLQQAGKLVGGDAEYTRFSG
                     SLEAYFPLCGWLTYHPRAAAGLSRPALPWSEKFYLGGMHSLAGYRTHQLAGDQMLVLS
                     QELRLALPLRFYLSLRYDVGDVHDALEEIRLSSVRHGAGISLALDAPIGPVEIGYGVS
                     ADDHEQLYLRAGFDF"
     gene            23946..24593
                     /locus_tag="PKM94_07515"
     CDS             23946..24593
                     /locus_tag="PKM94_07515"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOD66528.1"
                     /translation="MRISLIVSFVLLGLGCGAGERRPAVDAPDLAATLTQAAALVTDR
                     FQQDLRGELMAAMNTGGAAAAIEVCRTRAPEIAAQHSRVGAWSIARVSDRPRRPEQAA
                     DDRQRAALAKFSDTALADQALTEWLADERGDSSFVYYRPIRIGGLCLSCHGTPDQLAA
                     GVAERLAEVYPGDQATGYAVGELRGMFVVAMAWPAARAEAERLVQASRDTTGTAR"
     gene            complement(24624..>25604)
                     /locus_tag="PKM94_07520"
     CDS             complement(24624..>25604)
                     /locus_tag="PKM94_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66529.1"
                     /translation="MVHLIKTTALRFALSLALALAAASGGRAAKYAGEPFTLGAGARG
                     LALGGAVAAGPFDATAAYWNPAGMARLDGHYLTAMHAETFGSLLNHDYVGFTSASADS
                     ASPIRTYGFYVYYLGGGGIKITQLDEFNYPYVVEEESHADILIGAALAGRLADRLDFG
                     LTAKIIYRDIGVKSGQGLTVDAGLLYRAAAWADVGVMVTDVTTGFIRYGGDTLAGANT
                     ESIYPTVKPGLRLERRYREFTGRFMMSGDVKFESLEAAAQYWSGPLSLDTHFGWEIGY
                     RELLFGRAGFDIGRFTAGGGVDVRHVTIDFAYLRQTDFDDTFRVSAAYRF"
BASE COUNT         4260 a         8291 c         8700 g         4353 t
ORIGIN      
        1 agccagttct ccaccgggag cgaaaacatc agcagcacac aactcccgat cccgagcagc
       61 gggaccagcg ggaacagagg agcgcggaag ggccgctcgg cgtgcggatt cgtgtagcgc
      121 atgatgagga ccgccgcaca cacgatcacg aaggcgagga gagttccgat attcacgagc
      181 tccgcgagga tgcgcagggg gagcgcggcg gccatcaccg acacgaagat cccggtgaga
      241 atggtcgact tccacggcgt gcggaatttc ggatgcacgg cgccgaagaa cgacggcggc
      301 accatgccgt cccgcgccat cgctagcatc acccggggct ggctgagcat catcaccagc
      361 agcactgaag tgatgccggc aaccgcgccg agcgagatca ggaactgcgc ccagggcagg
      421 ccgatctgcc gaaaagcatc cgacacgggc gcctcgatgt tgatccggtc gtaggggacc
      481 atgccggtga ggacggcggc cacggcgagg tagagcacgg tgcaaatgat gagcgaggcg
      541 atgattccga tgggaacgtc gcggttgggc cgcttggcct cctcggcgtg ggtggagacg
      601 gagtcgaagc cgatgtaggc gaagaagatc atggcggccc ccgagagcat gccgagcggc
      661 gcgcccccgg cgtcggcctg gcccgagacg aggttaccga aaaaactgat gccggtgtat
      721 ccgtacggcg cgaacggtcg ccagtgatcg ggattgatga agaaggcgcc cacggcgatg
      781 acgaacagga cgatcgccac cttgacggcc accagcgcgg cattgaaccc ggcgctctcc
      841 cggatcccct tcaccagcac gaacgtgatc agacaggcga caacgaccgc cggcaggtcg
      901 atcaccgcgc cggtcgagac gaggtgtccc acgccgggat cgtagtcgaa gggagcggtg
      961 gacagcgcgt agggcaattt gaggccgaat attcccagga aatcctgaaa gtacttggac
     1021 cacccgtggg ccaccgtggc cgaactgacg gtgtactcga gaacgaggtc ccacccgatg
     1081 atccaggccg ggagttcccc cagggtggcg taggcatagg tgtaggcgga gccggcgatc
     1141 ggcaccatcg aggcgaactc ggcgtagcag agggcggcga agacgcaggc gaggccggcc
     1201 accatgaagg agagcatgag ggcggggccg gccttgtcat gggcgacctg cccgaccagg
     1261 acgaaaatcc cggtgccgat gatcgccccg accccgaggc tggtcagccg caccggaccg
     1321 aggatgcgcc ggagccggtt ctcccctttc atctcctcca ggcacagggc gagcggtttc
     1381 ttggcgaaca agggattggc catgggtctg cctccggatt gtcgtgtcgg tcaatttccg
     1441 ttgtccgtcc ccggtacccg ccgcgggcca tggccgccgg ccgggcacga accctgcatt
     1501 tttcgaaagt gcgaatttga ggaaaccctg tcaagaaatt ccgcccgcgc gccgccggcc
     1561 tagtccccga acccggccga gagactcccc tggatgatcc agtaggaggc gtcccaggcg
     1621 cccgcggtcg gccgcgagtc ctcgtccgcg tacgaatcgt aggtgatcgt gtggtactcg
     1681 gcgctcacgg tgaaggcgta ccagcggtag agtttcagaa actcgatccc gaacaactcc
     1741 ttctccccgc cgtcgaactg cacgccgagg tcgccgaaat actccgatgt tccccggccg
     1801 tagagaaggt agatccccat ggacttgtcc cgcagccagg tgaaggcggg gttaattttg
     1861 agcagccact cccaggaatc gacattcgcc tccacctcga cgcgatcggt cgaggccgtg
     1921 ctcgccgtgc ccaggtcata gtagaacttg tggccgaacc ctttttccac gcgatactcc
     1981 gcctgggcga tgtttctgta accgcagcgc acgaggtagg cgaagtcgac ccccgcgttg
     2041 gtcagcttga aggactcatg gaactcgtcg ctcaaccggc gttcaatgtc gccggagctg
     2101 aagacagcca gcccgccgcc gaccccggcg tggaggaaga actgctcggg gtaggcgggt
     2161 gcagggggtt ccgcctgcgc gcgcggcggg gtgggcggcg gctgcgccgc ggccgcaggg
     2221 gccagcagca gcaccgccgg cgcggccagc acggccatgg cggccccgag acgacccttc
     2281 cggaaacacg gcatcatagg cacttccctt cacaactgag gccggccgct ccatgcggcc
     2341 gcccggaata tagctcacgc cgacagcccc gtccagcaga tcatcgcccg gcgcaattga
     2401 caacggccgg ggcggggcgc tataatcggg agtccgcggc cgggtcgccg gctcggacgg
     2461 gagaggttat catggacggg gtcaagaaga tcgtcattgt ttccgacggc acggggcgta
     2521 cggccaagcg gctcatggac gcggttctct cgcagtatgc ccaggccgag gtggggttcc
     2581 gcctcgaggg cacgtttcag aatgtccgca accgcaaggc ggtcgacaag gtgctggcag
     2641 agatctccaa cgagtacctg gtgatctact cgtttatctc cccggacatc cacaagtact
     2701 tccaccgcag gctggccgag cgcgacatcc tccacatcaa cgtcctcgac ccgatgctca
     2761 agacgatgcg caagtttctc ggcgtccacc ccgactaccg cccgggcatc ctccaccgca
     2821 tcgacgaccg gtactacaag aaggtcgatg cgatcgggtt cacggtcgag cacgacgacg
     2881 gccggggggc gatgatcggg gacgccgacc ttgtcctggt cggcctgtcg cgcacgtgca
     2941 agacgcccat ttcgatgtac ctggcctgca accacgggct gaaagtggcc aacatcccga
     3001 tcgtgccggg ggtgacgacc gagctggcgc tcaagcacca cctcggccac cttccccgcg
     3061 agcggattgt cgggctcctg atggaccccg acatcctctc ccacgtgcgg gaggaacgcc
     3121 tggagctgct cgcccgcacc gacagcgccg tggccgaact gagcgactac gtggatgtgc
     3181 gggtgattcg ccgggactac cgctggtgcc gggagctgtt cgagcggaac gggtggcgga
     3241 cggtcgacgt gacccgccgg gcgatcgagg aggtggcggt ggagattctc gacgaactgg
     3301 ggatcccgcg ggaggagtga ccgcgggcta ttccagctgc ccgaacactt ccgacaggta
     3361 ctggttcaag tcaccggcga tcatggcgcg cgaggtgcgg gcatcggcgg cgaaatcccc
     3421 cgccatgccg tgcacgtaca cgccgcacac ggcggccgac agcggcccca tgccctgggc
     3481 gagaaacgag ccgatcgccc ccgtcaatac gtcgcccgtc ccgccgcagg ccatgccttc
     3541 gttgccggtg gggttgagga aacacgaacc gttgggcgcg gccaccaaag tcgggctccc
     3601 cttgagcacg agcaccgctc tgagttcttt cgcccatttc cgcacgagcg ccatccggtc
     3661 gtgaatcccg gccgggaccg tctccccggt gaggcgggcg aactcgcccg gatgcggggt
     3721 cagcacgagc gcggcgcccc cctcccgccc ggcgagcaga gcggcgtggc cggcgaacgc
     3781 gttgatgccg tcggcatcga tcaccgccgg tttggtcaga ccagccgtga agcgccgcac
     3841 gagttcgaag gtttcgcggt ggcggccgat tcccggtccc acggcgaccg cgtcatggtc
     3901 ctcggccaga cggcgcactt cgccgaggcc acgcagagcg agcgcccccc gcttggccac
     3961 gtcgggcagg gggtgcgagg tcgcctcggc gatgagcgcg gcgatgatcg ggagcacgct
     4021 ccgggggcag ccgatcttga ccagcccgca gccgcagcgg taggcggccg tcccggccag
     4081 ggccgccgcg ccggtcatgc cgagcgagcc ggccacgatc aggagccggc cgaatgtccc
     4141 cttgtgcccg tcgggcgggc ggtcgggcag ccattggcgc accatgccga gagtgatcag
     4201 gtcggagcgg aggtcgaatt tcgccagcac ttcatcgggc accccgatcg gcaccaccgc
     4261 caccgcgccg gccagttcgc gcccgggcga cacgtagagc ccgtacttgg gaagggccag
     4321 cgggaaagtg tagtgggcct gtacgaccgc cccctccccc tcgcccgtgt cggcgttgag
     4381 gcccgagggc atgtcgacag cgatgacggt ccggtcctcg tggcggttga tgtactcgat
     4441 cagctctccc gccagaccgc gcggcgcccc ggagaacccg gtgccgaaaa cggcgtcgac
     4501 aatgaagtca tcgggcagcg tctccgggag cgcctcgacc gccgtcacct cggtcatcgg
     4561 gaatccgagc tctttggcgc gttcgaagtt gaggcggcag tcctccgaca tctgctcggg
     4621 cgggcccagg tagtagacgg cgacctgggc gcccatgccg gcgaggtgcc gggcgaccac
     4681 gaaaccgtcg ccgccgttgt tcccctttcc gcagacgacc gcgactttgg cgccgcggac
     4741 tttggggatg acggcgacgg cgaggcgttc ggcgatcccc cgcccggcgt tctccatgag
     4801 gacgggaccg gggatgccgt ggacctcgat cgtttcccgg tcgatcgcgc gcatctgttc
     4861 ggcggtgacg agtttcatcg gcccggccgt ttccgtttcc gcccggccgc ggcgttggcc
     4921 gccgcgcccc cgcacacggc ggggcggtcg agcgcatatt tgattgcttc aatcgccgcg
     4981 gagaaatact taaaccggat cgccttcttg atctccggcg ggatctcggc gatgtctttg
     5041 cggttgtcct ccggcaggat gacctcctcg atgccggcgc ggtacgcggc gagcagcttt
     5101 tctttcagcc cgccgatcgg catgagctgg ccgcggagcg tgatctcgcc ggtcatggcc
     5161 aagcacggtt tcacgggacg ggcggcgaag agcgacgcga gggcgactgc catcgtgatc
     5221 cccgccgagg ggccgtcttt gggcgtggcg ccggcgggca cgtggatgtg gagggcgcgc
     5281 ttctcgaaca cggtcggatc gattccgagg gtttcggcgt tcgagcgcac gtaggagagc
     5341 gcggcctggg ccgattcttt catgacctcg ccgagctggc cggtcagcgt gaacgactcc
     5401 ttgccgacca tcgaggtcgc ctcgatgaag agcagttcgc cgccgaccga ggtgtaggcg
     5461 agtcccggca cgacgccgat ctgccccttg cgcatgagca cttcgcgggt gaagcgctgc
     5521 ggaccgagga gtttggggat ttccttgccg gtgacgacga ctttcgcaga gtctccctgg
     5581 gcgacctttc gcgccgcctt gcgggccacc gccgcgatct cgcgctcgag attgcgcagc
     5641 ccggcctcgc gcgtgtatcc ctcgatgatg gcggtgaccg ccgaatcctc gaccaccagt
     5701 tggtccccgg tcagcccgtg gttctcgagt tgtttgggga tcaggtgccg gcgggcgatc
     5761 tggagtttct cgatgtcggt gtagccggga atgcggatca cctccatacg gtcgcgcaga
     5821 acggcgggga tgggctcgag ccagttggcg gtcgtgatga acatcacccg ggacaggttg
     5881 aacggcacct cgagatagtg gtcggagaag gtgtcgttct gctcggggtc gaggacctcg
     5941 aggagggccg aggaggggtc gccgcggaaa tcggcgccga gcttgtcgat ttcgtcgagc
     6001 atgatcaccg ggttgttgct gccggcgcgg cggatcgact ggatgatccg gccgggcatg
     6061 gcgccgatat aggtgcgccg gtgcccgcgg atctcggcct cgtcgcgcat gcccccgagc
     6121 gagacgcgca tgaactcgcg tccgatggcg cgggcgatcg accgcccgag cgaggtcttc
     6181 cccacccccg gcggcccgac aaaacagagg atcggcccct tgatgtcggc tttcagtttg
     6241 cgtacggcga ggtattcgag gatgcggtcc ttcaccgtcg ccagatcata gtggtcgtcg
     6301 tcgagtactt tcttcgcccg ccgcagatcg agccggtcct cggtcgactt gctccacggc
     6361 atcgacacca gccagtcgag gtaagtgcgc gagacggtgt actcggccga cgaggggttc
     6421 atgtggcgga gccggtcgac ctctttgcgg gccgcctggg ccgccacctc gggcatgtgc
     6481 gcctcggcga tctttctctc gaactcctcg atttccgagg tctcctcccc ctgtccgagc
     6541 tcgcgctgga tcgctttcat ctgttcgcgg aggatgtagt cccgctgcga tttgccgagc
     6601 tcgctcgccg cctgcgcctg gatcttctgc gagagctgca gcacctcgac ctccttgttg
     6661 atcgcctggt gcagcttctg cagccgcctc agcacttccg tctcctcgag aaactcctgc
     6721 ttctgccgga tcgggatgtt gagattggag gcgatgaagt cggccagttt cgagggcgag
     6781 tcctggttga tcgccgagac gtgaaactcc tcggtcaggt agggggcgag ctccacgagc
     6841 tgctggacgc gtccgaggat gctgcgctgg agggcatcga tcttgatccc gtcgtcgatc
     6901 tgctcgcgga gttcctcgac ctccgccatg aggtagcgtt cggtgtgggt gaagcggacg
     6961 atgcggaggc gggcgagacc ctggatgaga aaccgcaccg tgccgtccgg gaaccgcagc
     7021 atcttgagaa tgcttccgga gcagccggtg tggtagagat cgtcggggcc ggggtgttcc
     7081 tgttcggcgt ccttctggag gaagagcccg atccgcgagc cgcgcatgag ggcctcgtcg
     7141 acgaggcggg cctgctcggc atcctgtatc atcagcggca ccacgaggta cggatagacg
     7201 atggtgcctt tcagcgggag aatgggcagg atgcgcgggc cggccgcctg cttcgcctca
     7261 tccggagtct gcggttggtc ggtcgtcatg gtcacaatac ctcattcggt gacgccccgc
     7321 ggggcggggc gggctgttgt cgcactttcc cgcgcggccg gcccggccgc ggcacgggcg
     7381 gggcgcgttt cggcatccct acacgttgaa gcggaacagg atcacgtcgc cgtcctgcac
     7441 cacatagtcc ttgccctcga ggcgcgtttt gccggccgct ttgagcgccg ccggcgtgcg
     7501 gtaccgggcg tagtcctcga accgcgtgac ctcggcccga atgaagccgc gctcgatgtc
     7561 gctgtgaatg acgccggccg cccgctgggc cgtcgtgccg gcgcggatcg tccacgcccg
     7621 cacctccggc ttggcggccg tgagaaacga aatcagaccg agcaggtcgt aggacttccg
     7681 gatcacctgg tcgaccgccg gcgtcgcgat cccgaggtcg gccaggaacg cggcccgctc
     7741 aatctcaggc agcgcggcca gctcggcctc gatccggccg cacacgaccg ccacatcccg
     7801 cttccccggc gccgcgaggc cggcataccc ctcgcgcatc gccccggccc ggtccagcgc
     7861 gtcttcggcg atgttaaccg cgatcaggag cggcttgagc gtcaggaagg agtatccccg
     7921 gagcgcttta aggtcctctt ccgccatgtc cagatcgatg agcggctgct ccccctcgag
     7981 ttgggcgcgg cacgtttcga gcagctcgag gtcgtgcgtc gccgacttgt ccccggtcag
     8041 tttcatcttc cgtcccttct tgtcgagatt gccttcgaca atggagaggt cggagaggat
     8101 catctcctcg gtcaaattcc ggagatcgcg ttccggcgcg gcgtcgggcg agaaggcatc
     8161 gaccaccagg atgagcgcct ccatgaggcg gacctcctgg ctgatctcga cggctccggc
     8221 ctctttcccc ttgccggtga acccgggagc gtcgagaaat tcgatttccg ccggcgtgat
     8281 cttcttggga tcctcgatct ccgccagcac ggccagccgg gggtcgggca ctttgataat
     8341 cgcccggtgg gccgcctgac tgaaatcgcc gaccgcctcc tgctgtccgg aggcggcgtt
     8401 gaaaatcgtc gtcttccccg cctgcggttt gccgatgatg ccgagtttca tagagcgtaa
     8461 ttaagggtgg ggcgaaggcg aaggcaaggc tgattccggc cggtcaggag gttcgcccgc
     8521 ttccgctcgg atctcatcgg atttccgccg gggccggacg gcacggcaat cgtgtgaaag
     8581 tgccgctgtg tccgccggct gcggctctgg ataacgggga ccacatcgcc cacgttgcgc
     8641 gggacaggtc gggcgaaggg ggaaagcgac cgcctgggac gatagaccgc aggcacgggc
     8701 tcttctgctg cgatagttgc ggagtggggg cagcccggtt aagtggtgaa ggggaaccgg
     8761 ctgcgccggt cctacttctt cttcaccaaa ccgcgcagca ctttcagatt ctcgatatcc
     8821 tccgtcagat catcgccttt gggcgtctcg atgatcatgg ggattttctt taaccgcctg
     8881 tcattaatga agttaccgaa tcccttcagg ccgatatgcc cccgcccgat atgctcgtgg
     8941 cggtcccgcc tggacccgag ctctttcagg ctgtcgttgg cgtggatcac tttcaggcgt
     9001 tcccaaccga tcacctcgtc gaactgcttc atcgttttcc ggtagtccgc cggttcgaca
     9061 atcgggtaac cggccgcgaa gatgtggcag gtgtcgagac acacgcctat ctgagccttg
     9121 ttgtcgacgc gatcgatcat atatgccagt tgctcgaaag tgcagccgag attgctcccc
     9181 tgcccggctg tcgtctccag gagcaacgtt accgtgttgt ccggcagttc gtcaaagaga
     9241 cggttgatgg agagggcgac gcgctgcatc ccgatctctt ccccgctccc gacatgcgag
     9301 ccggggtgga gcacgagagc cgggatccgc agcgtgcggc agcgctccat ttcctctttg
     9361 agcgaggcgt atgatttctc ggcgagggct tcatcggggg aagcgatgtt gatcaggtag
     9421 gaggagtgcg ataccgccgc ggtgacgccg gtttcttcga tacgggcgaa gaacaggtcg
     9481 atctcttcgg tggtgagttt tttggctttc cactggttgt tcgatttgtt gaacatctgg
     9541 atcgtatcgc acgtggcccg gtggccgcgc tcaatggcgg tgaagacgcc gccggcaatt
     9601 gactcgtgag ctccgaactg catgtggccc tcgctggttg atggtcaggt cggaaaaacg
     9661 cccgatgaac ataccggttt ttttcggcac gcgcaagccg cgggcgagcc tgatctgaca
     9721 taagcggcta ttaatcaatt tgttacgccg aagtcacgag agctcgagag tgagaaaaga
     9781 cacaaaatag gtgaaaaatc tttttttgaa gtgacagaat atgacacaat ggcggtgtac
     9841 actactatcg gtcttttgac cgacctgtcc caaccaaggt ggaccgaatc ccttagtagg
     9901 gcacaccggg gcggggtcgc gttgcgaccc tgccctttat ctatcgccgc atctcgtccc
     9961 ccgaaaggct cttaaggtgc cggcctatcc tttcacgggg cggtgcgaat cccaattgcc
    10021 gctctccccc actccccgca tattaactcc cgataaccag tgctgacagg gagctaccga
    10081 agattatgtg cacatttctg tccgtcgacg aggccgcatt caccgacact cactggcggc
    10141 agttccacag tttcctcgtg aacatgcacg cacggggatt cgggaatgtg ccgccgtcca
    10201 ccgcccaggc gcttcggcaa cgcatcgaag cgcgcctggc gttgcgggac ggattccgcg
    10261 agtgggtaat cgttaaggat tcagccgttg tcggctggtt gggtattcat gtccggccgg
    10321 gttcggagac tgcgccgccg gtgatgttcc tcgtgttcga cttcgtgccg gggctggagg
    10381 tcgcgccgct ctcggcccgc gcggcgggcc tggttgccgc cgcgatgcgg gagaaccggt
    10441 gcggcgaggt ccactgcatg gcggcggagg cccacctcga cagcctgcgg cagacctggg
    10501 gcgggcgaat tctcagccgg ctccaccgct accgtctcga ccgccggacc gccaaccggg
    10561 acctcatcaa cgagtggctg gcggagtttc cccgacaatt cccctcgctg cgcatcgaga
    10621 tattcaccga gatccccgaa cggcatctcg attcctggat cgccttgttc gggcagtttc
    10681 tggccgacat gcccaacgag ggaacggcgg cggcgccatt cacgatgacg cgcgcggagg
    10741 tgcgcaaatt cgaggcgctg cggcggcgca acgggcagta cctgtacacc ggggcgctgc
    10801 tcgacgggga gggccggatg atcgggcact cgaacatggc gacccatgac gacccgccca
    10861 cggagtggca ccaggcgatg accggaatcg agcgcgcctg gcggggccgg gggctctcga
    10921 agtggctcaa agcggccctg tttgtgcgca ccggcgagga tttcccgcaa agccgctaca
    10981 tctggaccga gatgcgcgcg gtcaacgagc cgatccatct cgtgaaccgg gctatgggat
    11041 tcgagctgtc cagcgagggg tacgagtggt cgattgaccg cgcggcgctt gaccggctgg
    11101 ccgggtagtc cggcggcggg acagggcagg cgcggccggc gaacgccgcg ccgccgtgct
    11161 gggtaatccg gttacaccgg ttcgacggcg tcgcgcggca cccagccctt gcggccgtcg
    11221 gcggtgcacg cccacaacca gcccgactcc tcgcggcggc cctcgaggat atcccccggg
    11281 tggacggtga gttcctcggg gctgtagtct cgtttgagaa agcaccagcc ggccgtcgcg
    11341 ccgcccgcgg ggtcttgcgt gatcgtgagc caggtttccg gcacccaccc cccgccgccg
    11401 tcctcagcgg tgcaccacac ccagccctcg tactcgctcg ggcggcgttc gaactggagg
    11461 cgttcgccgg attccagctc gcgcgacggg ccgccggcgg cctggtggtg tttgatgacg
    11521 cggaatttca tgtcgcggtt gctcctttct cgcgcgcgcc ggagcgccgt gttcggcgcc
    11581 cggcgggcct tcggttgccg cgctcccgct cactcgtcga cgatagcctc ggcttcgatc
    11641 tcgacatacc aatcggcgcg gacaaggccg gccacgccga tcatggtggc ggccgggttg
    11701 atctcgcgga aaaactcgcc atgggcgcgg ccgatttcgt cctggcgcgt gaggtcggtc
    11761 acatatatgc gggtgcgcac cacatcggcc gcccgcccgc cgagttgctc gatagcggtg
    11821 cgaatgatct cgaggcatcg catcgtctgc ccgtacggat cgcccggcgc gcccgtggag
    11881 ccatcggctg cgatcggccc tgtgcccgcc acatggatgg cgttgccgac ccggatcgcc
    11941 cggcagaacc cgaacggctc ctcgtagggg gagccggagg agacccggcg gcgtgcggga
    12001 ttgtcggatc gcggcgtcat gaccctattt caggttcaac gagagaatct tcacttcggt
    12061 ctggtcctgg atggcgtagc ggaccccttc ccggcccacg ccggagctct tcatcccgcc
    12121 gtagggctgg tggtcggcgc ggtaggtggg gatgtcgttg acgaccactc cgcccgcctc
    12181 gatccgctcg aaagcgtacc agatgtcctt gattcgctgg gtgaagatgc cggcctgcag
    12241 gccgtagtcc gagtcgttga tcgcatcgac cacacggtgg aagtcgcggt acttcatgag
    12301 gaccgcgagc ggggcgaagg cctctttgga gcagacatcc atgtcggcct tgacattctc
    12361 cagcagagtc ggctcgagcg tgcggccgtc acagagcgct ttgccgccgg cgatcacttt
    12421 cgctccctgg gcgaccgccc ggcggagggt gtcgagggtc gcctgcaccg acgcccggtc
    12481 gaccatcatc cccatgtcga cgttcgggtc gagcgggtcg ccgaccgtga tccgtttgac
    12541 ccgggctttc aaccgccgga caaactcatc gtacacgtcc tcatgcacaa aaatgcgctg
    12601 aaccgagatg cacgattgcc cggcgaccgc gaaggcgccg taggcgaggc gctcgacggc
    12661 atacgctagg tcggcatcgt cggccaccgc cactcccgcg ttaccgccga gttccagcac
    12721 gacctccttc tcggcggcgt gtttcttgat ccaccagccg accgctgagg agccggtgaa
    12781 cgtgatgagc cggacgcggc ggtcctccag cagcggcgcc gccgccgccg aggaaccggg
    12841 gaggatcgag acggcgccct tggggtggtc ggtcttgtcg atgagcgccg cgagcatgag
    12901 ggcgatgagc ggggtcttgc tggccggttt gaggacgata gtgttgccgg cggcgattgc
    12961 cggggcgatc ttgtgggcca ccagattgag cgggaaattg aacggcgcga tcccggcgac
    13021 caccccccgg gggaaccgcc gcaccaggcc gacgcgctcc tcgctccccg ccgcccagtc
    13081 cagatcgatg atctccccgc cgatccgcct ggcctcttcg gccgcgaccg tgaagacgcc
    13141 gatcgcccgt ttcacctcgg cccgcgaatc tctgtacgcc ttgccgagtt cgcggcacat
    13201 cattttcgtg aactgctcga tgttcttctc gagcccgttg gccaccgcga ggcaggtttc
    13261 cgagcgtttg tatgcaggga gatcgcgtgt gacggcgaat gtccggtgcg cgatttcgat
    13321 ggcctgcgtg tagtcctcgg ccgaggccga agccgcagcc ccgacgatct catcgttgaa
    13381 gggattggcg accgcgatct tgtcggcgcg gtcgacccat tcgccgccga ggtacatctt
    13441 atacgttctg gccatgggca actccgatgt gtgtatatcc tgtctgttcg ggactccggg
    13501 cggggattcg ccgccgcgcc gggggctccg accgtgctcc tgtataggga ttcggccggg
    13561 cgggcgcaag tcgattgtgg ccggtgggaa agtcttggac ttgacccaca gattagggag
    13621 ttcgagcaac actcgcgagc tcgtattcac gtgcgggaga tcctgggccc gctgtaagat
    13681 cttgcccgca ggttagataa gagccgcgcc cgctttaggc cgccacttct ggtcagcggc
    13741 accggttttg cctgaaacta ggtcagcgag cgttgcggaa aggagggata agatgccgat
    13801 gtcacgctgt cgggatcaac ggggcctgac cctgctggaa gtcctggtgg cgatggtagt
    13861 cataagcgct tccctgttgt tgctcctggg aatgggcgtg acggctcttc acggcaatca
    13921 ttgggccaac aagacgactg tggccacgca aatgatgcag cagcggctgg agcacataca
    13981 gagcacaggc gacttctcga acgggacggc atcggccgaa ggcgtcgatg tttcctggac
    14041 gtgcacgccg atcggcaact atctgcggcg cgtcgacatc agcgccaact gggtcgatgc
    14101 cggaactcgg agccgcacca actccctcac ggcgtacatc acctcggact ctctttagga
    14161 ggtgcgccta tggcacagac acgcgaacac acctccggcg cccgagggac atcgcttctg
    14221 gaagtgctga ttgccttggt ggtcatgggc gtcatcacga ccgcgctgtt tgggctctac
    14281 atcacccagc accgtaccta tatctcgcag gatgacgtga ccctggtgca gcagaacgcc
    14341 cgcgcctcaa tcgatgaatt ggcgcggcac attcgcatga ccggccacgg cttgccgctg
    14401 gaaatgatgc cgctttcggc gttcaatacc gacccggaca cgattgtcat caggtaccgc
    14461 acggacaact gtgagactta cctggcggag gcgatggcga cgccgtcctc ggatctcacg
    14521 tgcgccaccg atgtcggctg tttccagccc ggtgagtggg tctacatcct cgaacccgat
    14581 tcgggcggcg gcgaatggct tgcgctcagc gaggtgcaga ccggcgcacg ggtcttgagg
    14641 cacggcacga tggttctgtc caaagcgtac gccaaggatg cgctggtcct gaccgccagg
    14701 caagtgacgt tctatgtcga cacgacgacc gacccacagc acccggcgct gacggtcaag
    14761 gtgccgggcc aaacgccgca ggttttcgcg gaaaacgtag aggatcttca gctgcagtac
    14821 cgcatgaaga acggccttgt ggtcgatgtt ccggtcgtgg tgagcgatgt gcgcgaggtc
    14881 ctgatcagca ttacggcccg ctcaaatcgc ccggaccatg aaatggcgga ggtgacggga
    14941 cacagcgacg cttaccggcg gcgcacgttc acgacatccg tctttctgcg caacgtgggc
    15001 atctagttgg aggaaaagcg ctatgacgac gcgactgccg acaactgccg ggcaccgcgg
    15061 cttcgccacc ttcatcgctc tgatcctggt ggtgatgctg acgctgctcg gcatcgcggc
    15121 cttgtccacc tcggacgacg aaatgaagat agccggcaac gaactgcacg agatgaaggc
    15181 gttctatgcc gccgaggcgg ggctggcgga cgccgcggca gcgatgcggt acgagtatga
    15241 cagcaccggg ctgccgcctg cggtactccc ctccggcacc tccaacctga acaactgcct
    15301 ggtgaccttc accactgccg acgatggtgc ggcctctccg cgcattttga ccagcggcac
    15361 gctggccggg ttgcacgcgc tggtgaagtc cttcaccatc acctcggagg cggagaatct
    15421 tcaggacggc gcgaaggtga cgttgtcgca atcgtttgag gtggccctgg ttcccctctt
    15481 tcagtttgcc gtgttctacg acaacgatct ggaaatcgcg cccggtccgg agatgaacct
    15541 gatcggccgc gtacactcca acggcaacat gtacctgcag tcgaacaaca cgctccgcat
    15601 gaacagctac gtgaccgcgg cgggggacat tcgccacggc cgcaaaggtc ccggcagcgc
    15661 cgggtacggc gatgttcaga tcaagagcgc gtccggggcc tttgtctcga tgaagcaggg
    15721 atcgggttgg ctggatgcgg gcgacccgca gtggtatgat tcatcggtgg ggcgctggca
    15781 ggggcgggtg caggatgccg cccacggcca ggagtcgctc aatctgccgc tctcgcagga
    15841 ggcgggcggc gatccgcaca gattgatcga gcgggccgcc ggcaacgtgg attcctatga
    15901 gaacaaggcg accctgaaat tcatcgacgg ccgggctttc cagcgggtcg gcggcatctg
    15961 gaccgacatc acggccgaca tgacgtcccg caacatcatt tcctcctccg atgacaagtt
    16021 ctacgatcag cgggagaaga agtgggtgga ccccatggag ctcgacctgg gcaagctgta
    16081 tgatgagggc tatgggccgg ccaacggagt gatttacttc tccgaccaga cctcggactt
    16141 tccggccctc cggattaaga acgccgatga gttggacggg ggtctgtcgg tcgtcagcga
    16201 gaatcctatg tatgtcgtcg gaaactacaa tgcgactgac aagaaaccgg cggccctcat
    16261 tgccgatgcc atcaccttcc tgtcatccgc ctgggatgac agcaagagca cgctcagcaa
    16321 aactcagcgg gtcgctacca ctaccgtcgt caatgcctgc tacgcaaccg gaaacgtgga
    16381 aacgacctcc agcgattaca gcggcggatt cgagaacctc ccccgattcc tggagacctg
    16441 gaacgggaag acgctccggt ggaagggctc ggccgtcaac ctgtgggcgt cccgacaggc
    16501 tactggcacg tggaacggaa cctactacga cccgccgaac cgcgactggt tgtacgatac
    16561 tgacctcgat gatccctaca agctgcctcc ggaaacgcca atgatccgcg tgttccagcg
    16621 cagcgggtgg aagcaggagc acgtcgggta cgcagacgcg gacttctaaa aggctacccg
    16681 cactgaaaaa ccgaaaaccc gcctgcgggc gggttttctt ttgccggcct cagcgaatat
    16741 cgatgtcgta ggggaggcgg gccaggagct cgccctccgg gagatccgcc agcggagcgg
    16801 cggccgcccg cacctcctcg ccgacgagac cggcccagcg gcccagccag ccgagcacgg
    16861 gcacctgggg caggtcgatc agccgccggc gttcgggata gcgcacgacc cggtattccc
    16921 ggaggcccag gtgcgaggcg gtgtagtcga gcgcctgctt gaggccgccg agctcgtcga
    16981 ccagaccgtg ctcccgccct gcctgtccgg tccacacccg gccgcggccg agctggtcga
    17041 tgctgtcgcg gggaaggcgg cggttttccg ccaccagtcc gacgaagtgg tcgtagaagg
    17101 cgccgagcat ggcgccgagg cgggcccgtt cctcgtcggt gaacgggcgc atcgaagtca
    17161 tcatgccggc gtgccggcct cgggtgaaca gctctttgcc cagctcgatc ttgtcgtaga
    17221 gtccggccag caccggtttg ccgccgaaga tgccgatcga accggtgatg gtggcggggt
    17281 cggcgaagat gtgtctgccg gccatggcga tgtagtaccc gccggaggcg gcccgcgaac
    17341 ccatcgagac caccaccggc ttctgccggg cggccagctc ggcccgatgg aagatctcgt
    17401 tgccggccag ggcgtagccg ccgggagagt cgatgcggaa cacgactccc tgcacctgcg
    17461 ggttggcgag cgcctgcccg aaggcgctct ccatgagcgc cggggtcacc tcgcggccgc
    17521 ggcccgagag cgggctggga tccgcagccc cctcgggctg gatttccccg tcggcgtaca
    17581 cgacggcgat ttcgggcagc ggcggccaac cgtcgttcat cagcgtgtcg gcccggtagg
    17641 cgcggaaact gatttccggc agcggactga gaaacttgtc ggggaccaag cggtcccgat
    17701 aggtgagccc gtcgaccagg ccgaggtcga gggcctcagt cgaggtgtag ggcccgctgt
    17761 cgatcaggcc gcgcatggcc tccgggtcca tgccgcgccc ctccgccagg ccgcccacga
    17821 actgggcgta gaggtcatcg agcagccggt tggtcatctc ccggtttgcc tccgacggcg
    17881 tccgcatcgt gtacgcctcc ggggcggtct tgtagtcgcc gatacgtacc atgtccgcgg
    17941 ccacgccgag cttctccatc gtcccggcaa agaaagtcag ttcggcccgg aggccgatga
    18001 ggtcgaggtc gctgaccggc gggatgagga tcgagtcgcc ggcgcaggcg acgtagtagg
    18061 cgatgttgtt cggtccggtg aggtggctga cgatgctttt cccctgcgcc cggaaccacg
    18121 ccatggcctc gcgcatttcc tgggcctggg cgagggtgag gcggaggttg tccagatgga
    18181 gcaccatctc ccccacgccc gggtcgccgg ccgcccggta gatgccgagc acgagatcgg
    18241 cgaaggcggg acgccgcgcg ccgaagaatg tctgcaccgg gttctcgtcg gcagccccgc
    18301 tcacggacac ggacagacgg cgcgccgacc cgcggatcag cgacggctgg cgcgaatcgg
    18361 tggcccccac gacgaggtgg ctgaaacgat ggccggcgtc ggaatcgaag tggctctgcg
    18421 tcccgacgaa atactccagc aggttggccc gcgcgccgat ctcgaagttg ccgtcggagt
    18481 cgacgaacgc ctcgatggag aggccggtgg cggggatgac ttgcgcgtgg tagatgtagg
    18541 tccagtcatc ctcggcggcg gcggccgacg agagggcgtc gaccgcaagc gtaaccgtcg
    18601 ccccctgcgg ccggtagccg agcgaatagc gctggatgac gcgggtgtcc tcgccctcaa
    18661 gctcggaccg gttcaggttc gaccagaccg cgcccacggc gaaggggcta ttgccgcgcg
    18721 aggtgaggcc gatgttccag tagtggcggt gatcgtaggg actggggccg gacggaaagt
    18781 accggtagga accccccacc gagagcgagg aaccgagcga caggccgccg gcgagcgcgt
    18841 attccttgaa atcctccccg gcgtcgctgt caatgcgccg gtaagccagt ccgaatcccc
    18901 gtcgcgtgat gacggtgccc cagtttttca gaaccgaacc ctcggcgagg tcgcccatga
    18961 aagcgacggt ggcgggtttg taggggccga ggacggccgg gttgacccaa accgcttcgg
    19021 ggccgaacgc ggtggccgcg ggcgcatagt agaacacgtc gtcgggaaaa ggcacttcct
    19081 gcgccttcgc cccgagcggg atggccgcgg ctgccgcaag cagcgcggtc cagcgatgaa
    19141 accgcatcat tcctctccct ggcctcggcc acgaatgtag atgttgccgc tgccgctgat
    19201 ggaactgctg atgaatcctt gtcccccgcc gaccaccaat ccgaggcggt cgcgctggac
    19261 gaggtcggtc tggaaccgca gaccggacac ttcgatccgg ccgctctccc ccaccgcgag
    19321 cgacatcacc gccgacaggt cggcggcggc ggtgatgtcg acatcctcga accggttgct
    19381 caccaccaac tggctctcgc ccaggtcggc gatctcgagc gtgatcgggg cggactggcc
    19441 gcggatcaca ttgcgccgtc cgagcggccg gaagtcggtg acgtcgatga ggccgaaatc
    19501 gttgcggatg ctgacttcgc cggtgagccc ctcaatgcgg atctccccgc cgtcgttgcg
    19561 gaacaccgcc tggtgctccc ggctctcgat atcggaggcc gtgagggtcg agttgctcgt
    19621 ccggacggca atgtcgccgg cgacgtcctc gagaatcacg cggcggttgg ctgtgccgag
    19681 gtcgaccggt ccccgcaccc ggtagacctc caggcgcccg agcgacgacg ggttgaccac
    19741 cccgctgaac ggaccctcgg ccgtgacgtc aaaatagagc gcgtcgatat cgacgaagac
    19801 gttttcagga acgcggattt ccgcgtcgac gctccccgac tcggcgccgc tccacggcgc
    19861 ggggttgggc gcgcgcaact ggagccgggc gccgtcggca acgcggccga tgtcgacagc
    19921 gatgaggtcg atgtagtcga cggcgcggga gcgcgagtcg gtgacggcct gcttggtgta
    19981 gacgacgacg gcccggtcgg tttcggtagc gcggatggtg atctttccgg ccagcgtggc
    20041 ggccgagcgg acgagcagct tctcgccggg ggccagcggg gcggcgacag tcagcgggcg
    20101 ggtggcgaag cggccctcgc cggcgtcgtg aatttcctgc gcccgggccg ggccgatacc
    20161 caccactgcg gcggccaaca gcgcgcaggc ggcgcgccgg gccggtcggc tatgagagcg
    20221 agtcataaat ccctttcatg agtattgatc ccgcgaccgc cgcgttgagc gactcgacct
    20281 cgggggcgtg ggccacgcgg acggtgcagg ccgccagcgc ccgcatccgg tcggacaggc
    20341 cctccgcctc cgaccccacc gcgagcgcgt agcgggccgc cgggtccggg cggagagatt
    20401 cgctcagcgt ctgcgtcccc ccgcgcccgg ccgccagcag ggtgatcccc tcgctcgcca
    20461 ccagacgcgc gagggtgtcg cagtcggcgg cggcgatccg gacgccgaag acggccccgg
    20521 ccgacgaacg cacaactttg ggtgcaaacg gctcagccgt cgtcccgacc gtcaccacca
    20581 accggaaacc gaacgcgagc gcggaccgca ggagcgtgcc gaggttgccc ggatcggcca
    20641 cgttttcgca cacaagaatg gtacggtggc ggcgcgcaca aagttcggcc gggtcgatcc
    20701 ggggaatggt aaagacgccg acgatgccct gcggagcggg cgtgtcggcc agcttggaga
    20761 gctggcggac cgacagctcg cggcagggaa cccgggcggc gcgaaagcgc gccgccagtt
    20821 cgcggccgcg cgcgctgagg tcggcgggcg cgtagaacac ttcatccggc cgaaacgagt
    20881 gacgcagcga ctcctcgagc aggcggatcc cctcggcgag gaagcgtccc tgacgccgcc
    20941 gccccttctt ggtcaggagc gcctgcagat tttttaattc cgtgccggtg acctgcatat
    21001 tttattggcg gtgacacgta tcgccgcccc gataaggaat tgacgacccg ttaggcttgg
    21061 caagtgaaaa ctcccgcgtt gacgctcctg tgcagtctgc ttctggtgtc cctgctgcac
    21121 gccgagaacc gtccgatcat tatcggcggg ctcgacggcc ttgatccgtg ggcggaggag
    21181 gcgggttacc cggtgctcct ggcgctctcg ggcgggggca ttcgcgggct ggccgggatc
    21241 ggcgcgctgc gggcattcga agaaacgggt atccgggtgg tcgccatcgc cggcacctcc
    21301 atgggcgggg tcgtcggcgg gctttacgcc gccggctaca gcccggccga gctcgaggaa
    21361 atcgtgcacg ggcttgattt cgccgcgctc ttccagaaca ccccgccccg cgcgaccatg
    21421 ctgcagacgc ggcggcgcga acacgggcgc gacctgatca ggctgcgctt cgagggcctg
    21481 cggccgcaaa tcccgcaggc gctcacctcg gcccagtacc tcacgaacgt cctgacggag
    21541 ctgacgaccc aggcgacgta ccggtccgac ggggacttca gccgcctgcg cgtgccctac
    21601 gcggccgtca gcaccgacat catcagcggt cagcaggagg tgctgcggag cggctcgctt
    21661 gccgacgcca tgcgggcgac catggcgttc ccgctggctt tcaccggcct ggagatcgac
    21721 ggcaagtttc tcatggacgg gggcatggtg gatccggtgc cggtcgacgt ggtgcgcgat
    21781 ctgtccgact ccaccgattt cgtggtggcg gtgaacacgg cgagcacgct gcggagccgg
    21841 gaggagctgg tgacgccgct cgacatcgcc gaccaggcga cgacgatcat gacggcgcag
    21901 cggctgcggg agtcgctcga cgaggcgaat ttcgtcctcg agccggcgcc gcccggcatt
    21961 caatcgacgg actaccggtt cagcgacagc ctcatcgcgc tcggctaccg ggccgccctg
    22021 gcggcggccg acagtctccg gcgcctgctg gagcagcggg ttgaccacac tgagtttaca
    22081 gtcgcgagcg tggccgcggc gaccttcagc ccggaggccc aggcgatctt tcgggatgcg
    22141 ctcctgaatc gcccgttcac ccgcgccggg cttatccggg aactgaagcg gctggtgcgc
    22201 gctctcgacc tgttccgtct ggaggccgtg ctggagccgg cgacgggcgg cggcgcgccg
    22261 ggggaggcag ttgagcttcg cctcaatgga ttcgccaatt tcgcgcccgg tgccgcggcc
    22321 ttcctcttct tcggcaacac ccgtttcgac gacagcgaca tggaggaaac cctggcgctt
    22381 ggcgattcgg cggtgacgcc ggccagcctt cggcgcggcc tcgaccgcat cctcggccgg
    22441 tacgccgccg caggctggga cctcgcctgc atccgccgga cgtctgtcga tcccgaccgg
    22501 gcgcgcatca cgatcgtgat cgacgaagcg ctggtgcggg atatcgcggt ggacggcgtg
    22561 cggcgcaccc gcccctggta cgtgcgcgcc cactgcccgc tgcgatcggg ggagccctat
    22621 tcgacggcgc aggccgccgc gggactcaag aacatctacg gcaccgatct cttcgaccgg
    22681 gtcaccatcg acatccgccc ctccggcggg cggccggtcg tagtcatcgg cgtggaggaa
    22741 aaggcctacc cgcaggtgcg gctgggctgg cactgggacg acgagtacga gtcggaggaa
    22801 ttcatcgaac tggccgacga caacctcggc gggatcgggg tggaggggct ggtgcacgcg
    22861 cgctactccc ccgaccggca ggactatcat gtcgggttgc gcgccgaccg catattcaag
    22921 acctacctca cgagcacgat cagcgggtac cgtaccctcc tggaccgcca cctgtacgat
    22981 gccgatgact cgctgatcgg cgcgcgccgg gagtcgaagg ccggcatcga gatccgtctg
    23041 ggccagcaga tcgcccggct ggggacggtc acggccggcc tggtggtgga gcaggtcgag
    23101 tactcgcacg agccggacgg ctcggtcgag agcgatttcg gcctgcgcat cctcgaaatc
    23161 gagtcgatga tcgagacgct cgaccgggta ccgttcccgc gcaccggcga gcgcatctac
    23221 ctccagttgc agcaggcggg gaaactggtc ggcggcgatg ccgagtatac gcggttttcg
    23281 gggtcgctcg aagcgtactt cccgctgtgc ggttggctca cctatcatcc ccgggccgcg
    23341 gccgggctct cgcgcccggc cctgccctgg tcggagaaat tctacctggg cggcatgcac
    23401 tccctggccg gctaccggac gcaccagttg gccggagacc agatgctcgt gctcagccag
    23461 gagttgcggc tggccctgcc gctgcggttc tacctctcgc tccggtacga tgtcggtgat
    23521 gtgcacgacg cgctggagga gatccggctg tcgtcggtgc ggcacggcgc gggcatcagt
    23581 ctcgcgctgg acgccccgat cgggccggtc gagatcggct acggggtgag cgcggacgac
    23641 cacgagcagc tctacctgcg ggcggggttc gacttctagg ggcgcacggc ccccccttgg
    23701 tgtcacgcgg ctgccgcgtc ccggcctcct gtcggcgccg caattctcac cacgccgccg
    23761 ctcgaaagca ctctctgtgt cggggtgaat tctctgctgt ccgctcgtag aacacagcca
    23821 tcgggagttc agttcccggc gcgctccggg ccggggggtc tatgcccgcg cggggcggca
    23881 aagggtttgc ctgtgcggtc gcgatggtgt agcttctccc ggtgtccgga caaatcggag
    23941 gtgatatgcg aatcagcctg attgtttctt tcgtactgct gggtctcgga tgcggcgccg
    24001 gcgagcgccg cccggcggtg gacgcgcccg atcttgcggc caccctgacc caggcggccg
    24061 ctctcgtgac cgaccggttt cagcaggatc tgagggggga gttgatggcg gccatgaaca
    24121 cgggtggggc ggcggccgcg attgaggtct gccgcacgcg ggcgcccgag attgccgcgc
    24181 agcacagccg ggtgggggca tggtcaatcg cccgggtatc cgaccggccg cgccgcccgg
    24241 agcaagccgc cgatgaccgc cagcgcgcgg ccctggcgaa attcagcgac accgccctgg
    24301 ccgaccaagc tctcaccgaa tggctggccg acgagcgcgg cgactccagc ttcgtctact
    24361 accggccgat ccggatcggc gggctgtgcc tctcgtgcca cggaacgccg gaccaactcg
    24421 ccgccggcgt tgcggagcga ctcgcggagg tctaccctgg ggatcaggcg accgggtacg
    24481 cggttggaga gctccgcggg atgtttgtgg tcgccatggc ctggccggcg gcccgggcgg
    24541 aggccgagcg cctcgtgcag gcatcgagag atacgaccgg caccgcccgc taggagccag
    24601 gtgcgacggc cggggcgggc cggctaaaaa cgatacgcgg cgctgacccg gaaagtatcg
    24661 tcgaaatcgg tctggcggag gtaggcgaaa tcgatcgtga cgtggcggac atcgacgccg
    24721 ccgccggccg tgaaccggcc gatgtcaaag ccggcgcggc cgaacagcag ctcgcggtaa
    24781 ccgatttccc agccgaagtg ggtgtccagc gagagcgggc cgctccagta ctgggcggcc
    24841 gcctcgaggc tttcgaattt cacatccccg ctcatcatga accgccccgt gaactcgcgg
    24901 tagcgccgct cgagccgcag gccgggcttg accgtcgggt aaatcgactc ggtgttcgcc
    24961 cccgcgagcg tgtcgccgcc atagcggatg aaaccggtgg tgacgtcggt gaccatcacc
    25021 ccgacatcgg cccacgcggc ggcccggtag agcagaccgg catcgaccgt gagaccctgg
    25081 ccggacttca cgccgatgtc gcggtagatg atcttggccg tgaggccgaa atcgaggcgg
    25141 tcggcgagac ggccggcgag ggcggcgccg atcaggatgt cggcgtggct ctcctcctcg
    25201 accacgtagg gatagttaaa ctcatcgagc tgagtgatct tgatgccccc cccgccgaga
    25261 tagtagacgt agaaaccata cgtccggatc ggggaggcag agtccgcgga agcactggtg
    25321 aatccgacat agtcatggtt gagcagcgag ccgaaggttt ccgcgtgcat tgcggtgaga
    25381 tagtgaccgt cgaggcgggc catgccggcc ggattccagt aggcggccgt ggcatcgaac
    25441 gggccggccg ccacggcgcc gccgagggcg aggccgcggg caccggcgcc gagcgtgaag
    25501 ggttcgccgg cgtacttggc ggcacggccg ccggaggccg cggccagggc gagcgcgagg
    25561 ctgagggcga aacggagcgc ggttgtcttt ataaggtgca ccat
//
LOCUS       DAOPOE010000045        24780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2550_length_24780_cov_5.584146, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000045 DAOPOE010000000
VERSION     DAOPOE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24780
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_24780_cov_5.584146"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKM94_07525"
     CDS             complement(<1..186)
                     /locus_tag="PKM94_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOD66530.1"
                     /translation="MLATIEDIRACFRNDPACRNIEFLLYPGFHATTAHRFIHLLYKA
                     GIPFLPRLLSQIVRFLTG"
     gene            complement(259..1179)
                     /gene="cysK"
                     /locus_tag="PKM94_07530"
     CDS             complement(259..1179)
                     /gene="cysK"
                     /locus_tag="PKM94_07530"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007409942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOD66531.1"
                     /translation="MRFNDIVEAIGYTPLVRVNRLTDARSATVFLKMENFNPAHSVKD
                     RIGRSMIEAAERAGVLKPGMTIVEPTSGNTGIALAMVAAAKGYRCVLTMPDTMSIERR
                     ALCRQFGAEIVLTPGAEGMKAAIAKAREIAERGNCWIPMQFANPANPEVHRRTTGPEI
                     INDLGELRLDAFVSGVGTGGTITGAGAVLKAEYGCRIIAVEPTDSPVLSGGQPGPHKI
                     QGIGAGFVPENYDPAVVDEVIQVTNDDAFAMARELARQEGVLVGISSGAILWAALRVA
                     ERLGPGKNVVSIICDTGERYLSTPLFDKEP"
     gene            1594..1785
                     /locus_tag="PKM94_07535"
     CDS             1594..1785
                     /locus_tag="PKM94_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOD66532.1"
                     /translation="MKVRVDHELCSGDGICEELCPAVFKMNENDQADVIVDEVPPEEA
                     DAVREAAESCPEECIHIEE"
     gene            1919..2944
                     /locus_tag="PKM94_07540"
     CDS             1919..2944
                     /locus_tag="PKM94_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD66533.1"
                     /translation="MNILIIGGTRFMGPVVAAQLLADGHRVTVFHRGETEQDVPAGVH
                     HIHGDRGRLADYRRQWERLRPDVVLDMMALTGPQAGELVAAMSGLAGRLVAASSCDVY
                     RNFGLILRRETGRATSGRLTEDSPLRDHEYPYRQDAQGPADPFYDYDKIHVEREIQRG
                     PLPACAIRLPMVYGPNDPKHRLYSYARRMSDRRPALLLDTVRADWRGIRGYRDNCAHA
                     LCLAVARGAGRHRVYNVGEAEALTEAAWIRAIGAALGWEGDIVTVADRDLPDYLRTPL
                     DWRPQLDVDSSRIRNELGYGEIVDLQPGLARTLEWELAHPPDHPADRFDYAQEDAILR
                     RSRPSPS"
     gene            3001..4155
                     /locus_tag="PKM94_07545"
     CDS             3001..4155
                     /locus_tag="PKM94_07545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD66534.1"
                     /translation="MAADETFPIASYCSSQAWGGLEMNVLRFLHWMRGRGWPAVLFAR
                     PGSPLLTWAAAWGLPARALQSRSKASDLLGARTLARMAAADSARVLILHQSRDLLMAV
                     LTKIHTGNRLKVVYQQHMHIAGDKRDLLHAWQYRRLDAFVAPAPSLARQVAERTPIRP
                     ERLHVIPLGLEADRFLRKPPRSQARRQLGLPLDVPLAGIIGRLDPKKGQHVAIKALRR
                     VHETGRPLHLLVVGAPTRGEHADYEAHLHRLADDLGLAPFVHFRSHLDEPATAYAAMD
                     IFILASQSETYGMVTIEAMASELAVAGTDAGGTVDLIAHERNGLRFPPGDESACAAAV
                     IRFLADPHFAGRMAAQARRDVLANFTHDRQCAQWETLLRGLAGAAPAGSK"
     gene            complement(4244..7906)
                     /locus_tag="PKM94_07550"
     CDS             complement(4244..7906)
                     /locus_tag="PKM94_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD66535.1"
                     /translation="MRTLATAGLLLALAAAGGISAETPRHVENPSVTQGLPSFLGHVP
                     DRFIVVLKEGVAVDHAKDLRAPTALAALPGFADLQGRFQVKALRPQFPGADRQTRAAG
                     DAGRLARHYKVTIGTGSLDEAMAAYGALPDVDHVEPIGIHTLFALPNDPYYDNPPPEY
                     PYDQWHYWDSYGIRAHTAWDSETGDPAVIVGDLDIGTMYNHGDLGGSNPPGPNDASTN
                     GNIWVNAYEVPGNGIDDDGNGYTDDLIGWDFVDRTDWYSYPCIDIDCGGADNDPSDGN
                     GHGTHTAGTIAAITNNGYAVAGVAGGYGEGTFAGGGTGVKVVPCRIGYVLDYWIYGPT
                     GVVIMDYVAEAMYYMANLKLAGWNVAAINCSFGSSNSGGLGAAADFLIAQDVVICVAA
                     GNSSSSTPSYLGGRGDCLDVAATDQSGNNASFTNYGSWVDIAAPGVSVLSTITDPADP
                     GVDYIATMDGTSMACPHAAGVVALLESFNPGLSAAQKIALITDPANTNPYGGSRDLGA
                     GIIDARKCLDAAGGGCDLAADFSGTPVSGCLPLTVNFTDLSTGTGIDGWAWTFGDGGT
                     STASNPGHTYYAAGTYTVTLTITSSNQGCNAQAAKAAYITVNSLPSAAFVGSPTSGQA
                     PLTVTFTSQSTNATAFSWDFGDGGTSTAQNPSHSYAAAGTYTVALTASGACGSDTETK
                     VGYITVTEPPQLPPAAEFTGSPTSGTAPLTVQFTDLSANAPTAWSWDFGDGGASTAQN
                     PSHTYTAAGTYTVSLTATNAYGSDTETKLGYISVSELPQATRAYPSADLPAAGTVTGT
                     FARLAASDNVSQTITEVLYTGHPVKTYSYLEHKWTIDVGSGGAAMMFVVEASRPANSD
                     NDNIAFAYSTDNAAYTTLVTVASAAEQAYSAALPAGITGTVYIRAVDTDRNWGKTSLD
                     QVAIDQMYIEYTAGPTAPNAQFAGIPASGAAPLTVQFSDLSTGGPTAWSWTFGDGDAS
                     AAQNPSHAYSAVGTYTVTLTVSNAYGSDAETKTDYITVTDPNAAMHVGGMAVTRAKVG
                     ANYFGGCVVTIVDQNGSPVGGATVSAAYDGATSGSTSGVTAGDGTVTLRSSGVKKPSG
                     EWCFTVTNVAHASLAYQAAANAVTRACESGWVYGEDLGPLARTAPAGFDLAQNYPNPL
                     NPATEISFSLPTPQHATLEVFNVVGQRVAVLADASFGAGIHTVTWDASRVSSGMYFYR
                     LTAEGFVETKKMLLLK"
     gene            8200..9651
                     /locus_tag="PKM94_07555"
     CDS             8200..9651
                     /locus_tag="PKM94_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD66536.1"
                     /translation="MNVKALDDAGDRGRSRADWRWAVLLLLPFAPALLIAAHYRALVA
                     AAYFTALAALVMLAFPRLLFTLFLISIGLFLPYYVTDTMAISPADLLLALLAVIALLD
                     FLLRDSTAIRLSRIDGPFLALIGGTVISAFFAHNQAYSIIPVVRIVVVYAAYRLTFKF
                     GSELGVRCVLRRYVLFVAALSLINAVLFVLAGGRERVFGPAWLGYEPLSMTALPMAVT
                     FAVWAERHAERLRYIFAALLIGFGMLAAGSRGPMLAVLLVVPLLMFFGWRKARREQTI
                     TTRRVLRGMLLPLVAILALVIVLQELLFTGLIDRIRTLVESIGDPQETVLLRVVLAKA
                     AIGAFLSDPLTGIGIGNFRVVDEVVPQIRLEPVWFYIRGMSAHNVLLHYLAETGLLGA
                     VPLVLLALTGVRTAYRCFRRPLAHRHQQVSAALFAGMLVFAITLLYMRAWTWGQEGYV
                     MGLLFGLTAAWAWETEAGPPDTKGPRGYPPAET"
     gene            complement(9677..11857)
                     /locus_tag="PKM94_07560"
     CDS             complement(9677..11857)
                     /locus_tag="PKM94_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD66537.1"
                     /translation="MNRAVSIGTIIAAAAVLAAAGTVRGAESTTIASSDTVAASRPAG
                     DSAAAGPEVELGTVLVTATRSAHRKLDLPAAASSLSGASARSERLARTLPDALAEVPG
                     AMVQKTAYGQASPYLRGLTGFHTLLLVDGIRLNNSVFRSGPNQYWSTVDLHSVEAVEV
                     VMGPGSVLYGTDAVGGVVNVIPPHPSALLANRTARPGWQYRYASAEDSHTGRISLAAA
                     PAEHLGLAVGLTGKLFGDLRAGGGVGAQPKTGYDEWNVDVRAEYSPRRNSQLAGAYQR
                     TTQEDVWRAHKTIYGISWEGTTVGSDRRCVLDQERDLGYARYTVFDLSGRVQRLSGTL
                     SWHRQNEEQDRVKKDRTARRQGCTVRTVGLSVETELALLQARATVGIDLYRDRVSSWA
                     SDYDAAGALVQESAQGPVADRSTYDTWDFFAQAEWTLGRFSLLAGGRFSYVGVDAGRI
                     QDPLQADRVYSLERDWRHAAATGRIMWRPAEEWRIYGGVSHAYRAPNLSDLTRFDIAR
                     TNELEIPAPDLEPETFLCYEAGVKTRRGRWSGEVAVFYTAIRDLIIRVPTGEMLEDNY
                     VVTKRNAATGYVTGLEARAAVYLGAGLTVAAGGAWIDGRADSYPTSSGEEAREPLDRL
                     MPPEFTCEAVWRSADRRMWAGGELTVAGRQDNLSAADRQDTQRIPPGGTPGYAVVQVR
                     AGVKVGRAVTVSAALDNVFDAAYRIHGSGVNEPGRNLAVSLTWE"
     gene            complement(11894..13861)
                     /locus_tag="PKM94_07565"
     CDS             complement(11894..13861)
                     /locus_tag="PKM94_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD66538.1"
                     /translation="MVSLSDIVVEADRIPLAVWEVPAAVTVRALGPDRALRYRTPADL
                     AASLPGLRAYPSGNPWGQAVVDVRGFYGGGLAQYLLVSLDGIPLNDISTGLAPLTDLG
                     LEAIGRVEATRGPVSAQYGDFAMGGLLGFSTAERGHHRIGLALTGSADDALGAVVTIQ
                     RPAGPCDVLASGGVRRSHGWRAHSRFVQETGLVRVMSTAARAARLCGMLSFSHTEEQQ
                     PGAVTEAQLATDRAGAARDAYGNPLEDDAESRCFTAGFSGTGTLGRRGQWHGSSWLRV
                     TDADKIVTTTQAMDYRPRVISAGGEGAVEMQATAAGRPWRTSLGVSLDCGRLDSEYRE
                     RQSKNVIVGGSGWRAAVAAFARTRLELMPRLAVTGGLRADHAATEFEYEATNLTALST
                     TQSQSWFRLSPSVAVSVAAGRGVQCYGMVSGAFKSPTLNHLYGSAPFNAGPPTNSFIL
                     LSNSNLEPMTGTAWEIGTKFRNRRGGYGTVSIYHYRLREEIDFDQGAMCYINVGRSHH
                     TGVEIAGGGPVRPDLTVEFSGIYTRAVIRSGEHADHRLAGVPEWSYRAEAVYAVAPGL
                     AAGVLISGRIDQWLDAANTRRLSDHAEVAVMCGYGWRAFRLDVRIDNLFDERYEYDGY
                     FDPLLAQVGQQPYRYYPAAPRQATVSLRVEL"
     gene            14259..14960
                     /locus_tag="PKM94_07570"
     CDS             14259..14960
                     /locus_tag="PKM94_07570"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOD66539.1"
                     /translation="MTHSLFPALVSLLAAVAAAGVRSETDVPSPPADTLVRYVSDHEI
                     RVGNVLMDISALTVSAPGWVNQKTGLVEYLAVTSEGKRHESVLVLDVQPLHLQTALLL
                     FGLDFGQNLAFQGDSVPPRGDSVDISVAWLGPEGDSVVVPASELLFDYHDSVVAPTTP
                     WVFTGSMIHLDRLQADLEGSIIATYSDPVAILNNPASARSDDTFYGVNEALVPPPGTP
                     VVLIITVPLRTGEDK"
     gene            14957..16135
                     /locus_tag="PKM94_07575"
     CDS             14957..16135
                     /locus_tag="PKM94_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOD66540.1"
                     /translation="MKTFAAVAILAAFPLLIAPPLPAQPAHPFDLSLRHEVERSVAAG
                     LQYLAGTQKDNGSWSYYVGITGLATTAFLQAPSAVKEQYWANIDRGLAFVLANVRPNG
                     GLYPDIEPQLHAYNTAICLMALVAADNPAYKDIIINARDYLLSLQADEDEGISPDSST
                     YGGIGYNRDERSDLSNMQFALEALRASEDFKPTAEYAGKVEYRGDAVQETAEVSNKEL
                     FWEKAILFLQRSQNYRKYNDYEWSSDDGGFIYYPGSSKAGGTTSYGGMTYAGMKSFIH
                     AGLRREDERVQKAYAWIRANYTVEENPELGRQGLFYFYSVMAKALSLFGDSLLVDSGG
                     VAHDWRGDLADKLISIQQSDGSWVNENGRWWENNPDLVTAYCVMALEKLLQPLAPKME
                     "
     gene            16483..19713
                     /locus_tag="PKM94_07580"
     CDS             16483..19713
                     /locus_tag="PKM94_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD66541.1"
                     /translation="MNDVAVFSMRLARLLAALAVVAILTGGAAAASGSANPTGQPKPA
                     GSQDVLPPAFSEGFTLPAPEAVALEPEIVPSLGDPKVAPSVNVMPGKFVGRGEPYTWV
                     GRILTIWGNVKNGAPPLTFEWQFGDGSAPVTGSVSNAKYIATTHTYASSGIKRAVLTV
                     TDADGFSDRDTVIIDVIGTPTLKDSINASVEDGLRYLYNSQNTGGLWIGYSSYYVSAT
                     AQAVLCFENQGYLPSDDPNENIYVECIQQGLDYLTANLRPVAIGLQTYCDPEAVAPGN
                     TDANGLGIYPVSGSPYEAGPIMMALAGSGDAMKTHPAGTGVANVIGRSYYEILVDMVD
                     WCAWAQNESGRGGWRYSANYSSSDNSVSQWPTIGLEAAEKQWGISPPPCVKSELRLWI
                     NASQLANGRFTYYPSTYATSYVPMTGAGLCEMSFVGIPTDSGRVKRAMQQLFLGWSDY
                     YGKGFPYAMYGIKKGCDIARPEPIEDLDTTAGYFDWYSDYARYLLAPGRQNATTGQFL
                     IHHGYLGDARIHDHTWCILVLEPVTCVPDAIINSADTVGVSQPFVLDGSASNHPCGEI
                     LQWRWDFDVSDGIDFDNPDAIGQVVTHPGVPLPPGDPLGDVTVALRVYDDNDPVQTGD
                     DQKTIVISRGCKAPVAVLGGPYAGHPGDVITFDACASYDPDAEDPDSECPDSIVSFAW
                     DFNGDGIYNDYADSASCTPAHSWPAPTEDYICVEVRDSYGKADSLCVPVKISPIDLDL
                     QPENISFNPATGTEGDLITIRADIFFNSEFDTTIHNVLIRFYDGDPNVLVQQIGPDQI
                     LASIDSGQTVVLEVNWTVPDLLPHDIYIVVDPLGTIPEWDESNNRAYRTWVPGQCEED
                     RLVVIPWVQYEFYARHIGPLTLDSLCLWADDSCTWLQTPITVNPLQSYAWIGNFADPY
                     GPDDVDTATLMINDAVPLHGFAADLVGSPESFPFLDQIFPGQLVLRMRYSIPALVQYY
                     IDSLAALPEPELMWDEAVYPLAVKGQYLDGTAFNLCTDLIIIGHRSGDVNGDDSRTVT
                     DITFLIGYLFRGGPAPDPVYLGDVNHDGQTTVADITRLVGIIFRGE"
     gene            complement(20211..21149)
                     /locus_tag="PKM94_07585"
     CDS             complement(20211..21149)
                     /locus_tag="PKM94_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOD66542.1"
                     /translation="MKSYFRILLGSKNCFAEEAYRSNCIAAGFITDRDLTHHLPDNWR
                     DFNREFIPVWMKQNPGKPKVSAGLACGMLWTVAKGVQVGDVVLCPDGKGNYFVGEVTG
                     GYEYKKGEELPHRRVVRWFDRPLTRDEMSEPLRKSLSSGPPVINVSRHAAEIERLIAG
                     GQPLTIVSTDATVEDPSAFALEKHLEDFLVQNWHHTELGKHYDVFEEDGELAGQQYQT
                     DTGPIDILAVSKDGKEIVVIELKKGRASDVVVGQVQRYMGYVKEELAESNQNVRGVII
                     AFEDDIKLRRALAVAPNIDFYTYKVHFKLERKNQRR"
     gene            complement(21533..21742)
                     /locus_tag="PKM94_07590"
     CDS             complement(21533..21742)
                     /locus_tag="PKM94_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD66543.1"
                     /translation="MRGNVDFDEEDKVTVVDITFLINHVFRGGPAPDCLAEADVTADG
                     KLAVADITFLIAYVFRGGPAPVACP"
     gene            complement(21773..22003)
                     /locus_tag="PKM94_07595"
     CDS             complement(21773..22003)
                     /locus_tag="PKM94_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66544.1"
                     /translation="MNDAVNALTVFNGALIAGGYFTAAGGTSANYIAASDGSTWSPLG
                     SGMNDWVNALTVFDRTLIAGGRFTVAGSLVSA"
     gene            complement(22174..24249)
                     /locus_tag="PKM94_07600"
     CDS             complement(22174..24249)
                     /locus_tag="PKM94_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOD66545.1"
                     /translation="MKRLHLISKLISAMLIVACAAALALAAVPTTFTYQGRLTTSAGA
                     NVPDGSYLIRFVIYNAPTEGVVLWDNGYRHVTVIGGLFAYTLGDSTALPSTLFTGDTT
                     RYLGIKVGADPEIIPRVKLNTVPFAFKALFADTAAMAFGVAGGGGGWTDGGPTVRLTT
                     LTDKVSIGTNGANNKLDVTMTDATGGTAYFNLDNAANPTPALYSATSGSGHGISGSAV
                     SGSSAGVFGYCGSGYGVQGSSASKYGIYGTGSKGVFGEHPTSHNYGYLGGLNDGVFGK
                     DTSSGCSGTLGGEYFGVAGSAQQANEVGIFGECTSGVGIRASSTDSTGVYASSAFGLG
                     VYGLGRRGVYGKGSTYGIMGEHSSSGNYATLGEDTRAILAEAIGKYDEGLLALNDSGG
                     AIVTIASNRTGVLAQTDTGSGHWADCSGALAYYGKAVCGFGDSAGFFTGNVKITRFSE
                     HIGGLTLHTDSHNDPGRCGIFFSNNQLGTLEGDDTEDQYFGFYSGFTGNRSYDAHLAV
                     YGRNPSGWGSRIEMYHNGTDGYLATDVGDIALLPNLNVGVGTSTPGYKLDVAGSCHAT
                     SFPTSSDDRLKANVAPIVDALEKIEQINGVSFDWNERYDSLGRSSGHREIGVIAQEVE
                     KVLPELVTTWGDQNYRAVDYGRLTAVLVEAVKQLAKENSSLNADNSAIRQRLDELQHK
                     VEQLMKVDQ"
BASE COUNT         4182 a         8346 c         7746 g         4506 t
ORIGIN      
        1 cccggtgaga aagcggacga tctgcgacag caggcggggg agaaagggga ttcccgcctt
       61 gtacaggagg tggataaacc gatgggcggt agtggcgtgg aaccccggat agagcagaaa
      121 ctcgatgttc cggcaggccg ggtcattgcg aaaacacgcc cgaatgtcct cgatggttgc
      181 cagcatagtc gttcccgact tccgatcttg ggttgggttg acggatctgg tgcattactc
      241 tcattgtcgg ccgcgggcct acggctcctt atcaaaaagc ggggtcgaga ggtaccgttc
      301 gccggtgtcg cagatgatgc tgaccacgtt cttccccggt ccgagccgct cagccacccg
      361 cagcgccgcc cacaggatag cgcccgagga gatcccgacc agtacccctt cttggcgcgc
      421 cagttcgcgg gccatggcaa aagcatcgtc gttggtcacc tgaatgactt catccacgac
      481 cgcggggtcg tagttctccg gcacaaaccc cgccccgatc ccctggatct tgtgcggccc
      541 cggctgaccg cccgacagca ccggagaatc ggtcggttcg acggcgatga tgcggcagcc
      601 gtattcggct ttcaacaccg cccccgcgcc ggtgatagtt ccgccggtgc ctactcccga
      661 cacgaaggca tcgaggcgca gctcccccag gtcgttgatg atttccggtc cggtggtccg
      721 gcggtgcacc tccgggttgg ccgggttggc gaactgcatc ggaatccagc aattcccgcg
      781 ttcggcaatc tcccgggctt tggcgatggc cgctttcatg ccttccgctc ccggcgtcag
      841 gacaatctcg gccccgaact gccggcacaa cgcccgccgt tcgatactca tggtgtcggg
      901 catggtcaac acgcaccggt accctttcgc cgccgccacc atagccagcg cgattccggt
      961 attccccgag gtcggttcga caatcgtcat cccgggtttc agcactcccg ctcgctcggc
     1021 cgcttcgatc atcgaccgtc cgatgcggtc tttgaccgag tgcgcgggat tgaagttctc
     1081 cattttgaga aacacggtgg cggaacgggc atcggtcagg cggttgacgc gcaccagcgg
     1141 ggtgtacccg atagcctcaa caatgtcgtt aaatctcatt cgactgactc ctataagttg
     1201 ttatcgtcca tctacctgag ttcattgagg ggccttgccc cggacggacc gttcggcccg
     1261 gcgcgggaca cgcgagtgcc cggccgccgc cgcggcgcac atcagggcat ccccagaaat
     1321 gtcatgtgct tcatgaagac cctcagatac tataacaaat tacgccgccc aacgcacccg
     1381 gaaatttccg ccggtcacgc ccaaaaaaaa tcggctgagg gcttgacgga agtcaaaagc
     1441 cgcccttgac atcacccggt ttttgtgtat gcttcgcttc gaaatcaggc cgaaggcgcc
     1501 caacacgccg gagccgggag cgtgggacgc catgacctgt gaagcgaaag catgtgaacg
     1561 acatacatcc gagatgaccc taaggaggtt acgatgaagg tacgcgtgga ccacgaactg
     1621 tgcagcggcg acggcatctg cgaggagctc tgcccggccg tgttcaagat gaatgagaac
     1681 gatcaggccg atgtgatcgt tgacgaagtt cctccggaag aagcggatgc cgttcgggaa
     1741 gccgccgaga gctgccctga ggaatgcatc cacattgaag agtgagcgct gacttcccgc
     1801 ccggcgggaa cgacagatat gcctggagcg cggccgcgtg cgaccgcgct cttttcttct
     1861 tccccttcga ccgggatcaa ggagctccga cccggccgca gcgcagcgca ctgcccgtat
     1921 gaacatcctc atcatcggcg gcacccgctt catgggaccc gtcgtcgccg cccaactcct
     1981 ggcggacggg caccgtgtga ccgtgtttca tcgcggcgag accgaacagg atgtgcccgc
     2041 gggcgttcac catattcacg gcgaccgcgg ccgtctcgcc gactaccgcc gccagtggga
     2101 gcggctgcgg ccggatgtcg tgctcgacat gatggccctg accggaccgc aggccgggga
     2161 actcgtggcc gcgatgtccg gcctcgcggg gcggctggtg gcggcgagca gttgcgatgt
     2221 gtacaggaat ttcggcctga tcctgcgccg ggaaaccggc cgcgcgactt ccggccgcct
     2281 caccgaggac tccccgctgc gggaccatga gtatccctac cggcaggacg cccagggacc
     2341 ggcggatccc ttctacgact acgacaagat ccatgtggag agggagattc agcgcgggcc
     2401 gctgcccgca tgcgcgatcc gcctgcccat ggtctacggg ccgaacgatc ccaaacaccg
     2461 gctctacagc tacgccaggc gcatgagcga ccggcgcccc gccctcctgc tggatacggt
     2521 gcgcgccgac tggcgcggaa tccgcggcta ccgcgacaat tgcgcccacg ccctctgtct
     2581 cgccgtcgcg cggggagcgg gtcgccaccg ggtctacaat gttggggagg cggaggcgct
     2641 gaccgaggcg gcgtggatca gggcgatcgg cgcggccctc gggtgggagg gggatattgt
     2701 caccgtggcc gaccgcgatc tgccggacta tctccggacc cccctcgact ggaggccaca
     2761 gctcgacgtc gattcctcgc gcattcgcaa cgaactgggg tacggagaaa tcgtcgatct
     2821 ccaaccgggg ctggcccgca cgctcgaatg ggagctggcc catccgccgg accacccggc
     2881 cgaccgcttc gactacgcgc aggaggacgc catccttcgg cggagccgcc cctcgccctc
     2941 ctgagcgggt cgggcgacaa agagcttggc cgcggcggcg gccccccgat atattggcgc
     3001 atggcggccg acgagacctt cccgatcgct tcgtactgct cctcgcaggc ctggggcggg
     3061 ctcgagatga acgtcctgcg ctttctccac tggatgcgag ggcggggctg gccggcggtc
     3121 ctcttcgccc ggccgggcag tccgctgcta acctgggccg cggcatgggg cctgcccgcc
     3181 cgggcgctcc aatcgcgctc gaaagcctcc gacctgctgg gggcccggac cctggcccgc
     3241 atggccgccg ccgacagcgc gcgcgtgctc atcctgcacc agtcgcgcga tctgctgatg
     3301 gcggtcctca ccaagatcca caccggcaac cggctgaaag tcgtctacca acagcacatg
     3361 cacatcgcgg gagacaagcg cgacctgctc cacgcctggc agtatcgccg gctcgatgct
     3421 ttcgtcgcgc ccgctccgtc gctggcccgg caagtggcgg agcgcacccc catccgcccc
     3481 gaacgcctcc atgtcatccc cctgggcctc gaggccgacc gttttctccg gaagcccccc
     3541 cgctcgcagg cccgccggca gctcgggctg ccgctcgacg ttccgctggc ggggatcatc
     3601 gggcggctcg atccgaagaa ggggcagcac gttgcgatca aagcgctgcg gcgcgtacat
     3661 gaaaccggcc gcccgctgca cttgctggtc gtcggtgcac cgacacgcgg cgaacacgcc
     3721 gactacgagg cgcacctcca tcgcctggcc gatgacctcg gcctcgcgcc gttcgttcat
     3781 ttccgctccc acctcgatga gccggcgacg gcctacgccg ccatggatat cttcatcctc
     3841 gcctcgcaat cggaaaccta cggcatggtg acgattgagg cgatggcgtc ggagctggcg
     3901 gtggcgggca ccgacgcggg ggggacggtc gacctgattg cccacgagcg caacggcctg
     3961 cgctttccgc cgggggacga gtccgcctgc gcggccgcgg tcattcgctt tctggccgac
     4021 ccgcactttg cggggcggat ggccgctcag gcgcggcgcg acgtcctggc gaatttcacg
     4081 cacgaccggc agtgcgcgca gtgggagacg ctcctgcgcg gtctcgcggg cgcggccccg
     4141 gccggatcca aatagcaggg gcaggagcgt actgctcctg cccctggcca cgggtggctt
     4201 gttcgcccgc ccgccggcgg ccggcgtgcg gcttctcccg ctgctatttc aacagcagca
     4261 tcttctttgt ctcaacaaag ccctcggccg tcagacggta gaaatacatg cccgaggaca
     4321 cccgcgaggc atcccaggtc accgtgtgta ttcccgcgcc gaagcttgca tcggcgagca
     4381 ccgccacgcg ctgcccgacc acgttgaaca cttcgagagt cgcgtgttgc ggggtcggca
     4441 gcgagaagct gatctcggtc gccgggttga gcgggttcgg gtagttctgg gcgaggtcga
     4501 agcccgccgg ggcggtccgg gccagcgggc caaggtcctc gccgtacacc cagccgctct
     4561 cgcaggcccg ggtcacggcg ttggcggcgg cctggtaggc gaggctcgcg tgcgcgacgt
     4621 tggtcacggt gaaacaccac tcgccgctcg gcttcttgac accgctcgac cgcagcgtca
     4681 ccgtgccgtc gcccgccgtc acgccgctcg tgctgccgga ggtggcgccg tcgtacgcgg
     4741 cggagacggt cgcgccgccc acgggcgatc cgttctggtc gacgatcgtg acgacgcacc
     4801 ccccgaagta gttcgcgccg accttcgcgc gcgtcacggc catgccgccg acgtgcatcg
     4861 cggcattcgg gtccgtgact gtaatgtagt cggtcttggt ctcagcgtcc gacccgtagg
     4921 cgttcgacac cgtgagcgtg accgtgtacg tgcccacggc gctgtacgcg tggctcgggt
     4981 tctgcgcggc cgaggcgtcg ccgtcgccga aagtccagct ccacgcggtc gggccgcccg
     5041 tcgacaggtc ggagaactgc accgtcagcg gcgcggcccc cgaggcggga atgcccgcga
     5101 actgcgcatt cggcgcggtc ggaccggcgg tgtactcgat gtacatctgg tcgatggcga
     5161 cctgatccag cgacgtcttt ccccagttgc ggtcggtatc gacggcgcgg atgtacacgg
     5221 tgccggtgat tccggcgggc agggccgcgg aatacgcctg ctcggccgca ctggccaccg
     5281 tcaccagcgt cgtgtacgcc gcgttgtccg tcgagtaggc gaaagcgatg ttgtcgttgt
     5341 cgctgttggc cgggcgagac gcctccacga cgaacatcat ggcggcgccg ccgctgccga
     5401 cgtcgatcgt ccacttgtgc tcgaggtagc tgtaggtctt gaccgggtgc ccggtgtaca
     5461 gcacctccgt gattgtctgg ctgacattgt cgctcgccgc caggcgggcg aatgtgccgg
     5521 tcaccgtccc cgccgccggc agatcagccg atggatacgc ccgcgtcgcc tgcggcagct
     5581 cgctcaccga gatgtatcca agcttggttt ccgtgtccga gccgtacgcg ttggtcgcgg
     5641 tcagggacac cgtgtaggtt ccggcggccg tatacgtgtg gctcgggttc tgcgcggtcg
     5701 aggcgccgcc gtcgccgaaa tcccagctcc aggcggtcgg cgcgttggcc gacaggtcgg
     5761 tgaactggac agtgagcggg gccgtgcctg aggtcgggct gcccgtgaac tcggccgcgg
     5821 gcggcagctg cggcggctcg gtgacggtga tgtagcccac tttcgtctcg gtatccgatc
     5881 cgcacgcccc gctcgcagtc agcgccaccg tgtaggtccc ggcggccgcg tacgagtggc
     5941 ttgggttctg cgcggtcgac gtgccgccgt caccgaagtc ccaactgaag gccgtcgcat
     6001 tcgtcgactg actggtgaaa gtcaccgtga gcggcgcctg gcccgaggtc gggctgccga
     6061 cgaatgccgc cgagggcagc gaattgacgg tgatgtacgc cgctttagcc gcctgggcgt
     6121 tgcagccctg gttgctgctg gtgatggtca gcgtgaccgt gtaggtcccc gccgcgtagt
     6181 aggtgtggcc ggggttcgag gcggtcgacg tgccgccatc gccgaacgtc cacgcccaac
     6241 cgtcaatccc cgtgccggtc gacagatcgg tgaaatttac ggtgagcggg aggcatccgg
     6301 agacgggcgt gccgctgaaa tcggcggcca ggtcgcagcc gccgccggcc gcatcgagac
     6361 acttgcgggc gtcgatgatc cccgctccga ggtcgcgcga tccgccgtag gggttggtgt
     6421 tggcaggatc ggtgatgagg gcgatctttt gcgcggcgct cagccccgga ttgaacgact
     6481 ccagcagcgc gaccacgcct gccgcgtgcg ggcaggccat cgaggtgccg tccatggtcg
     6541 caatgtagtc aacgccggga tcggccggat cggtaatcgt gctgagcacg ctcacccccg
     6601 gcgcggcgat gtccacccac gagccgtagt tggtgaaact ggcgttgttg ccgctctgat
     6661 cggtggccgc cacatcaagg cagtcgccgc ggccgccgag gtagctcggc gtgctcgaac
     6721 tggagttgcc ggccgccacg cagatgacga cgtcctgggc gatgaggaaa tcggccgccg
     6781 cgcccagccc gccgctgttg gagctgccga aggagcagtt gatggcggcg acattccagc
     6841 cggccagctt caggttggcc atgtagtaca tggcctcggc gacataatcc atgatcacga
     6901 cgccggtcgg tccgtagatc cagtagtcga gcacgtaacc gatccggcac ggcaccactt
     6961 tcaccccggt gccgccgccg gcgaaggtgc cttcaccgta gccgccggcg acccccgcca
     7021 cggcgtagcc gttgttggta atcgccgcga tcgtcccggc cgtgtgcgtc ccgtggccgt
     7081 tgccgtcgga gggatcgttg tcggcgccgc cgcagtcgat atcgatgcag gggtaggaat
     7141 accagtcggt gcggtcgacg aaatcccagc cgatgagatc atcggtgtag ccgttgccgt
     7201 cgtcgtcgat tccgttgccg ggcacctcgt aggcgttgac ccagatgttc ccgttggtgc
     7261 tcgcgtcatt cggtcccggc gggttgctcc ccccgagatc gccgtgattg tacatggtgc
     7321 cgatatcgag atccccgacg atcacggccg ggtcgcccgt ctcgctgtcc caggcggtgt
     7381 gggcacggat cccgtaagag tcccaataat gccactggtc ataggggtat tccggcggcg
     7441 ggttgtcgta gtaagggtcg ttcgggaggg cgaagagggt gtggatgccg atcggttcga
     7501 cgtggtcgac atccggcagg gcgccgtagg cggccatggc ctcgtcgagc gatccggtcc
     7561 cgatcgtgac tttgtagtgc cgcgcgagcc ggccggcgtc ccccgcggcc cgcgtctgcc
     7621 ggtcggcgcc ggggaattgc ggccgcaagg ctttcacctg gaaccgcccc tgcaggtccg
     7681 caaaaccggg cagggcggcg agcgccgtcg gcgcccgcag atccttggcg tggtcgaccg
     7741 ccaccccttc tttcaggacc acgatgaatc gatcgggcac atgacccaga aaactcggga
     7801 gcccctgcgt cacactgggg ttctccacgt gtcgcggggt ttcggcagag attcctcccg
     7861 cggcggccag ggcgagaagc agcccggccg ttgccagcgt tctcattgtt cctcctgtga
     7921 gtcttcctgt tcttgtccga atggtgagtt ccgtgattca cggtcaaagg ctcatgacgg
     7981 caagattgtc tgggtcgcat cacctcctcg gacgccggac cggcggattc gtcgggcgct
     8041 gcatcctgct cttcgtatta aatcgatgcg atacggcctg ctgaacgggc ttcgttctca
     8101 atatacgccg cgcggaggtt ccgacaagtg aaaagagggc gggcgccggg cggcgaagaa
     8161 actgttgtcc cggcgggcgc catcggcgat attcgcccca tgaacgtgaa ggctctcgac
     8221 gacgcaggtg atcgcggccg ctcccgggcc gactggcgct gggccgtgct gctccttttg
     8281 cccttcgcgc cggcgctcct gatcgcggcg cactaccgcg cgctggtcgc cgccgcctac
     8341 ttcaccgccc tcgccgcgct cgtgatgctc gctttccccc gcctcttgtt caccctgttt
     8401 ctcatctcca tcgggctgtt cctcccctac tacgtcaccg acacgatggc gatcagcccg
     8461 gccgacctgc tcctggcgct gctggcggtg atcgctctgc tggacttcct cctgcgcgat
     8521 tcgacggcta tccgcctgag ccggatcgac gggccgtttt tggcgctcat cggtgggacc
     8581 gtgatctccg ctttcttcgc ccacaaccag gcttattcga tcatccctgt tgttcgtatt
     8641 gtggtggtct acgcagccta ccgtctgact ttcaagttcg gcagcgagtt gggggttcgt
     8701 tgcgtgctaa ggcggtacgt tctgttcgtg gcggcgctct cgctcatcaa cgcggtgctg
     8761 tttgttctcg ccggcgggcg cgagcgcgtc ttcgggccgg cctggctggg ttacgagccg
     8821 ctgtcgatga cggcgctgcc gatggcggtg acgttcgcgg tgtgggccga aaggcacgcc
     8881 gagcggctcc ggtacatctt cgccgcactc ctcatcggct tcggtatgct ggcggcggga
     8941 tcgcgcgggc cgatgctcgc ggtgttgctg gtggtgccgc tcctgatgtt tttcgggtgg
     9001 cgcaaggccc gccgcgagca gaccatcaca acccggcgcg tgctgcgcgg gatgctcctc
     9061 cccctggtcg ccattctcgc cctcgtcatc gtgctccagg aactcctgtt cacgggtttg
     9121 atcgatcgga tccggactct ggtcgagtcg atcggcgacc cgcaggagac cgtactgctc
     9181 cgcgtggtgc tggcaaaagc ggcaatcggc gcgttcctca gcgatcccct caccggcatc
     9241 ggcatcggca atttccgggt tgtcgatgag gtcgtgccgc agattcgcct cgaaccggtg
     9301 tggttctata tccgcggcat gtcggcgcac aacgtcctgc tgcactactt ggcggaaaca
     9361 ggcctgctgg gcgccgtgcc gctggttctt ctggcgctca ccggcgtgcg caccgcgtac
     9421 cgctgttttc ggcggccgct tgcccaccgc caccagcagg tatcggcggc gctgtttgcc
     9481 ggcatgctgg tcttcgcgat cacgctcctg tatatgcggg cgtggacctg ggggcaggag
     9541 ggctatgtga tgggtctgct tttcggactc accgccgcct gggcctggga gacggaagct
     9601 ggccctccgg atacgaaagg accgcgcggt tacccgccag cggagacttg attggcctgg
     9661 ggcgagcccc accccctcac tcccaggtca ggctcacggc caggttccgc cccggttcgt
     9721 tgacgcccga accgtggatc cggtacgccg catcgaacac gttgtccaga gccgcgctca
     9781 ccgtcaccgc gcgccccacc ttcacgcccg cccgcacctg caccaccgcg tacccgggcg
     9841 tccctcccgg cggaatgcgc tgcgtgtcct ggcggtcggc tgcggacagg ttgtcctggc
     9901 ggcccgccac ggtcagttcc cccccggccc acatgcgccg atcggccgag cgccagaccg
     9961 cctcgcacgt gaattccggc ggcatcagcc ggtcgagcgg ctcccgcgct tcctctcccg
    10021 aggaggtcgg gtagctgtcc gcccgtccgt cgatccaggc gccgcccgcg gccaccgtaa
    10081 gccccgcacc gaggtacacc gccgcgcgcg cctccagtcc ggtcacatag ccggtcgcgg
    10141 cattgcgttt ggtcaccacg tagttgtcct cgagcatctc gccggtgggg acgcggatga
    10201 tgaggtcgcg gatggccgtg tagaaaaccg cgacctcccc cgaccaccgc ccccggcgcg
    10261 ttttcacgcc ggcttcatag cagagaaagg tttcgggctc aaggtcgggg gcggggattt
    10321 cgagttcatt ggtgcgggcg atatcgaagc gggtgaggtc cgagaggttc ggcgcccggt
    10381 aggcgtgcga cacccctcca taaatccgcc actcttcggc cggccgccac atgatgcggc
    10441 cggttgcggc cgcgtgccgc cagtcgcgtt ccagcgagta cactcggtcg gcctgaagcg
    10501 ggtcctggat gcgcccggcg tcgaccccca cgtacgagaa tcgccccccg gccagcaggc
    10561 tgaaccgccc gagggtccat tcggcctggg cgaagaaatc ccaggtgtcg taggtggagc
    10621 ggtcggccac cggcccctgg gccgactcct gcacgagcgc ccccgccgca tcgtagtccg
    10681 aggcccacga ggaaaccctg tcccggtaga ggtcgatccc gaccgtggcg cgggcctgca
    10741 ggagcgccag ctccgtctcg accgacaagc cgaccgtacg caccgtgcac ccctgccgcc
    10801 gcgcggtgcg gtccttcttc acccggtcct gttcctcgtt ctggcggtgc cacgacaggg
    10861 tgcccgagag ccgctggacc cggcccgaca ggtcgaacac cgtgtaccgc gcatatccca
    10921 ggtcgcgctc ctggtccagc acgcaccggc ggtcgctgcc gacggtcgtg ccttcccagc
    10981 tgatcccgta gatcgtcttg tgcgcccgcc agacatcctc ctgcgttgtc cgctggtatg
    11041 cgcccgccag ctggctgttt cggcgcggcg aatattcggc gcgcacgtcc acattccact
    11101 catcgtagcc ggttttcggc tgcgcgccga ccccgcctcc ggcgcggagg tcgccgaaga
    11161 gtttccccgt gaggccgacc gcaaggccga ggtgttcggc cggcgccgcg gcgagactga
    11221 tccggcccgt gtgcgaatcc tcggcgctcg cgtagcggta ctgccagccg ggacgcgccg
    11281 tgcggttcgc caggagcgcc gagggatgcg gcgggatcac attcacgacc cccccgaccg
    11341 catcggtgcc gtagagcacc gatcccgggc ccatgaccac ctcgaccgcc tcgaccgaat
    11401 ggaggtcaac cgtgctccag tactggttgg gaccggaacg aaagaccgag ttgttcaacc
    11461 ggatgccgtc gacgagcagg agcgtgtgaa acccggtaag cccccgcaag tacggagagg
    11521 cctggccgta cgccgttttc tgcaccatgg cgccgggaac ctcggccagc gcatccggaa
    11581 gcgtccgcgc cagccgctcg ctccgcgccg aggctccgct gagcgaggag gcggcggccg
    11641 gaagatcgag cttgcggtgg gcgctgcggg tggccgtcac gagaactgtc cccagttcga
    11701 cttccggccc ggcggcggcc gaatccccgg ccggccggct cgcggcgacg gtgtccgatg
    11761 aggcgattgt ggtgctctcg gcgccgcgca ccgttccggc ggcggccagc acggccgctg
    11821 ccgcaatgat cgtgccgata ctcactgctc tgttcacctt gcctctgccc ttcatcactt
    11881 gcaggcgcgc cggttagagt tcaacccgca gcgagacagt tgcctgccgg ggcgccgccg
    11941 gatagtaccg gtacggctgc tgtccgacct gggcgagcaa cggatcgaag tacccgtcgt
    12001 attcgtatcg ctcgtcaaaa aggttatcga ttcgcacatc cagccggaac gcccgccagc
    12061 catacccgca catcaccgcg acctccgcat ggtcggagag ccgccgggtg ttggcggcat
    12121 ccagccactg gtcaatccgc ccggatatca gaacgcccgc cgccagaccc ggcgccacgg
    12181 catagactgc ttccgcccga tacgaccact ccggcactcc ggccagccgg tggtcggcgt
    12241 gctcgccgga ccggatcacg gcgcgggtgt agatgcccga aaactcaacc gtcaggtccg
    12301 gccgcaccgg cccgccgccg gcgatttcca cacctgtgtg atgcgaccgt ccgacattga
    12361 tataacacat ggccccttgg tcgaaatcga tctcctcccg cagccgatag tggtagatgc
    12421 tcaccgtacc gtagccgccg cggcggttgc ggaatttcgt cccgatctcc cacgcggtcc
    12481 cggtcatcgg ctcaaggttg ctgttcgata gcaggatgaa ggagttagtg ggcgggccgg
    12541 cgttgaatgg agcggatcca tacaagtgat tcagggtcgg ggacttaaag gcccccgaga
    12601 ccattccgta acactgcacg ccccgtccgg ccgcgacact gactgcgacg ctcggcgaca
    12661 accggaacca tgattgagat tgggtcgtgg agagggcagt caggttggtg gcctcatact
    12721 caaactcggt agcggcgtgg tcggcgcgaa gcccgccggt tactgccagc cgcggcataa
    12781 gttcgagccg ggtccgggcg aaagcggcca ccgccgcgcg ccagccggag ccccccacga
    12841 tcacgttttt cgattgccgt tcccggtact ccgagtccaa ccgcccgcaa tccagtgaca
    12901 ccccaaggct tgtgcgccag ggtcggccgg ccgccgtcgc ctgcatttcc acggccccct
    12961 ctccgcctgc cgatatgacc ctggggcggt aatccatcgc ctgcgtcgtc gtcacgatct
    13021 tgtcggcgtc ggtcacccgg agccaggagc tgccatgcca ctggccacgc cgcccaagcg
    13081 ttcccgtccc cgagaaaccg gcggtaaagc agcggctctc ggcgtcatcc tcaagagggt
    13141 tgccgtacgc atcgcgcgcc gctcccgccc gatcggtcgc cagttgcgcc tccgtcaccg
    13201 cccccggctg ctgctcttcg gtatgcgaaa acgagagcat gccgcacagg cgcgcagcgc
    13261 gggccgccgt cgacatcacc cgcaccaggc ctgtttcctg cacaaaccgg ctgtgggccc
    13321 gccatccgtg gctccgacga acgccgcctg aggccagcac atcgcacgga ccggccggcc
    13381 tctggatagt gacaaccgca ccgagcgcat catcggccga tccggtcaac gccaggccga
    13441 tccggtggtg tccccgctcc gcggtcgaga atccgagcaa cccccccatc gcgaagtcgc
    13501 cgtactgggc cgagaccggg ccgcgcgttg cctcgacccg cccgatcgcc tccaagccaa
    13561 ggtcggtcag cggcgcgaga ccggtcgaga tgtcgttcag cgggatgccg tcgaggctga
    13621 ccagcaggta ctgggccagc cccccgccgt agaagccgcg cacatcgacc accgcctgtc
    13681 cccacgggtt gccggaggga tatgcgcgga gaccggggag tgaggcggcc aggtcggccg
    13741 gggtgcggta gcgaagcgcg cggtccggcc cgagcgcccg gaccgtcacc gccgccggga
    13801 cctcccagac ggcgagcggg atccggtcgg cctcgaccac gatgtccgac agcgagacca
    13861 cggctgtatc cggctggccc tgagccggca ccgcggcggt gtcgcgagcg gcctggggag
    13921 cgcccacagc cccaggtccg atcaaagccg ctcccgctgt cacgatgcag agtgtggccg
    13981 cacgaaagta catacagggt gttttcggca ctccgaagtc gtcgatgtga gccgggcctg
    14041 gggaaaccgc ctgcgtcttg ccgggcgccc cgccgcctgc ccacaagata ccatcgtttc
    14101 gcgcgaagtc aaggcgcgga tcgtagtata attgaacgat ttatccttga cagcgcagcg
    14161 ttccgtgcgt agcttgggcc aagacaaaga gcagggcgag gaccctttct tcgagcgact
    14221 ctgacccagt gccgggccgc cgatccgaac agaatgttat gacacactcc ctgttccccg
    14281 cgctcgtgag cttgctggcg gctgtggctg ccgcgggcgt gcggtcggaa accgatgtac
    14341 cctccccccc cgcggacacc ctcgtccggt acgtgagtga ccatgagatt cgcgtcggga
    14401 atgtcctgat ggacatctcg gcgctgacgg tttcggcgcc cggctgggtg aaccagaaaa
    14461 ccggtctcgt cgagtacctg gcggtcacat ccgaagggaa gcgtcatgag tcggtgctgg
    14521 ttctcgatgt ccagccgctc catctccaga ccgctctgct cctgttcggt ctcgatttcg
    14581 gccagaacct cgccttccag ggggactccg tccccccgcg cggggacagc gtcgacattt
    14641 cggtcgcctg gctaggcccg gagggggatt cggtggtggt gccagcctcc gagctgctct
    14701 ttgactatca cgactcggtg gtggcgccta ccactccgtg ggtgttcacc ggctccatga
    14761 ttcacctgga tcggctccag gccgaccttg agggatcgat catcgcgacc tattccgatc
    14821 ccgtcgccat cctcaacaac ccggcctcgg cccggtcgga cgataccttc tacggcgtga
    14881 acgaagcgct cgtcccgccc cccggcacac cggtggtcct gattatcaca gtgccgttgc
    14941 gcacgggaga agacaaatga agacgttcgc ggccgtcgcg atcctggcgg cgtttcctct
    15001 cctgatcgcc ccgcccctgc ctgcccagcc ggcgcacccg ttcgacctct cgctgcggca
    15061 tgaggtcgag cgaagcgttg cggccggcct gcagtacctg gcgggcacgc agaaagacaa
    15121 cggctcgtgg agctactacg tggggatcac cgggctggcg acgaccgctt tcctccaggc
    15181 gccgtcggcc gtgaaggagc agtactgggc caacattgac cgcggcctgg cgtttgtgct
    15241 cgccaatgtc cggcccaacg gcggcctcta ccccgatatc gaaccgcaac tgcacgccta
    15301 caacacggcc atctgcctca tggccctcgt ggccgccgac aacccggcct acaaagacat
    15361 catcatcaat gcgcgcgatt acctcctcag ccttcaggcc gacgaagacg aggggatcag
    15421 tccggacagc tcgacctacg gcggtatcgg ctacaatcgc gacgagcgct ccgacctgtc
    15481 caacatgcag ttcgccctcg aggcgctgcg ggcatccgag gacttcaaac cgaccgccga
    15541 gtacgccggc aaggtggaat accgggggga cgcggtgcag gagacggctg aagtctccaa
    15601 caaggagctt ttctgggaga aggccatcct gttcctccag cgttcgcaga actaccgcaa
    15661 gtacaacgac tacgagtggt cgagcgacga cggcggcttc atctactacc cgggatcgtc
    15721 gaaagccggc ggcacgacgt cgtacggcgg catgacctat gccggcatga agagtttcat
    15781 ccacgccggg ctgcgccggg aggatgagcg ggtgcagaaa gcgtatgcct ggattcgcgc
    15841 caactacacg gtcgaggaaa atcccgagct cggccgccag ggtctcttct acttctacag
    15901 cgtgatggcc aaggcgctct cgctctttgg cgacagcctg ctcgtcgaca gcggcggcgt
    15961 cgcccacgac tggcggggcg atctcgccga caaactgatt tcgatacagc agtccgacgg
    16021 ttcctgggtc aacgagaacg ggcgctggtg ggaaaacaac cccgacctgg tgacggcgta
    16081 ctgcgtgatg gctctggaga aactgctgca accgctggcg ccgaagatgg aatagaatga
    16141 caacgcggtg gcgccggcac cgccgcaaga gaccggcagt cgccggtccc ggcgggcgcg
    16201 gcgaagggag gtgactgatc gggggagcga ggtaatcgga gtgcgcatcc gcgcggcccg
    16261 ggccgggaac gcaaccgccg gctggacagg cgaccgcctg cggccgcccg acgcctcgcc
    16321 acggatgcgc cgcaggatcc tcaggcatga gaacgccggc gctctcttcg gccggctccg
    16381 gctgtcgccc ctgcggctgc gaccgacggc cggctcaaga agtccggctc gtgaatacgg
    16441 atgaatttgg acagatgctc gcttcgagca aggtggattc acatgaacga tgtagcggtc
    16501 ttttcgatgc ggctggcgcg tctcctcgcg gcgctcgccg tcgtcgcgat tctcaccggc
    16561 ggcgccgcgg cagcctccgg cagcgcgaac ccgacggggc agccgaaacc ggcgggcagt
    16621 caggacgtgc tcccgcccgc gttcagcgaa ggtttcactc ttcccgcccc cgaggcggtg
    16681 gccttggagc ccgagattgt tccgtcgctg ggcgacccca aggtggctcc cagcgtcaat
    16741 gtcatgcccg gcaaattcgt gggccgcggc gaaccgtaca cctgggtcgg gcgcatcctg
    16801 accatctggg gcaacgtcaa gaacggcgcg ccccccctga ccttcgagtg gcagtttggc
    16861 gacggcagcg ccccggtcac aggatcggtg agcaacgcca aatatatcgc cacgacccac
    16921 acctatgcgt cctccggcat taagcgcgcc gtcctgaccg tcaccgatgc cgacggattc
    16981 tccgaccggg ataccgtcat catcgacgtc atcggcacgc cgacgctcaa ggacagcatc
    17041 aacgcctcgg tggaggacgg actgcggtac ctctacaatt cgcagaacac cgggggtctc
    17101 tggatcgggt actcgagtta ctatgtcagc gcgaccgcgc aggcggttct ctgttttgaa
    17161 aatcagggct accttccgag cgacgatccg aacgagaaca tctacgtcga gtgcatccag
    17221 caggggctcg attacctcac cgccaacctg cgaccggtcg cgatcggcct gcagacgtac
    17281 tgcgatccgg aagccgttgc tcccggcaac accgacgcca acggtctcgg catctacccc
    17341 gtctcgggat ccccgtatga ggccgggccg atcatgatgg cgctagccgg ttcgggcgac
    17401 gcgatgaaga cgcatcccgc cggcaccggc gtcgccaacg tcatcggccg ctcctactac
    17461 gagatcctcg tcgacatggt cgactggtgc gcctgggccc agaacgagtc cggccgcggc
    17521 ggctggcgct attcggccaa ctacagctcc tcggacaact ccgtctccca gtggccgacg
    17581 atcggcctgg aggcggccga gaagcagtgg ggcatttcgc cgccgccctg cgtgaaatcg
    17641 gaactgcggc tctggatcaa cgccagccag ctcgccaacg gacgcttcac ctactacccg
    17701 agcacgtacg ccaccagcta cgtgccgatg accggagccg gcctgtgtga aatgtccttt
    17761 gtcggcattc ccaccgacag cggccgggtc aagcgggcca tgcagcagct cttcctcggc
    17821 tggtccgact actacggcaa ggggttcccg tacgccatgt acggcatcaa gaagggctgc
    17881 gacatcgccc gccccgaacc gatcgaggat ctcgacacca ccgccggcta cttcgactgg
    17941 tattccgact acgcccgcta cctgctggct cccggccgcc agaatgcgac caccggccaa
    18001 ttcctcatcc atcacggcta cctgggcgac gcccgcatcc atgaccacac ttggtgcatc
    18061 cttgtgctcg agccggtcac ctgcgtcccc gacgccatca tcaactccgc cgatacggtc
    18121 ggcgtctccc agccgtttgt gctcgacgga tccgcctcca accacccctg cggagagatc
    18181 ctccagtggc ggtgggattt cgatgtcagc gacggcatcg atttcgataa tcccgacgcc
    18241 atcggccagg tcgtcaccca tccgggagtc cctctcccgc cgggggatcc gctgggcgac
    18301 gtgaccgtgg ctctccgcgt ctacgacgac aacgacccgg tgcagaccgg cgacgaccag
    18361 aagacgatcg tcatttcgcg cgggtgcaag gcgccggtcg cggtgctcgg cggaccctac
    18421 gccggccacc ccggcgacgt catcaccttc gacgcctgcg cgtcgtacga tcccgatgcc
    18481 gaggatccgg attccgagtg ccccgactcg atcgtctcgt tcgcatggga cttcaacggc
    18541 gacggcatct acaacgacta cgccgactcc gcttcctgca cccccgccca ctcgtggccg
    18601 gcgccgaccg aggattacat ctgcgtcgag gtgagagatt cttacggcaa ggccgactcc
    18661 ctgtgtgtgc ccgtcaaaat ctcgcccatc gatctggatc tgcaacccga gaacatctcc
    18721 ttcaacccgg cgacgggaac ggagggggat ctcatcacca tccgagcgga catcttcttc
    18781 aactccgagt tcgacacgac catccataat gtgctgatcc gtttctacga tggggacccg
    18841 aacgttctcg tccagcagat cggccccgat caaatcctgg cgagcatcga ttccggtcag
    18901 accgtcgtgc tcgaggtcaa ctggacggtt cccgacctgc tcccccacga catctacatc
    18961 gtcgtcgacc cgttgggcac catcccggaa tgggacgaga gcaacaaccg ggcgtaccgg
    19021 acctgggtcc cgggccagtg cgaggaggac cgcctggtgg tcatcccctg ggtgcagtac
    19081 gagttctacg cccgccacat cgggccgctg acgctcgatt ccctgtgcct gtgggcggac
    19141 gattcctgca cctggctcca gacgccgatc acggttaatc cgctccagtc ttatgcctgg
    19201 atcggcaatt tcgcagaccc ttacgggccg gacgatgtcg acacggcgac gctcatgatc
    19261 aacgatgcgg tcccgctcca cgggttcgcc gccgacctgg tcggctcccc cgagtcgttc
    19321 ccgttccttg accagatttt ccccggccag ttggtgctgc gcatgcgcta cagtattccc
    19381 gccctcgtgc agtactacat cgactcgctg gccgctctcc ctgaaccgga actcatgtgg
    19441 gacgaagcgg tctatccgct ggctgtgaag gggcagtacc tcgacggcac cgcgttcaac
    19501 ctgtgcaccg acctgatcat catcggccac cgctcgggcg acgtcaacgg cgatgacagc
    19561 cggacggtca ccgatatcac gttcctgatc ggctacctgt tccggggcgg cccggcgccg
    19621 gatccggtgt atctcggcga cgtcaaccac gacggtcaga ccactgtggc tgatatcaca
    19681 cgccttgtcg ggatcatctt ccggggcgag tagccgtccc gaccgcgggc gcggcacggc
    19741 cgcgcgccaa tcggctcaca ctaaccggct ggagcttcgg ctccggccgg tttcgtttgt
    19801 ggccgcaggc gacggccgag ggctccgccg attatcccgc cgatcccccc gctgatcctc
    19861 ccgccgatcg cctctcttat gggccgggac catgccccgc ttccgcacgc cgcacgccgc
    19921 cgcgcaaacc acgagatccg taggggagag gagagagggg aggagaagat catgccgaag
    19981 gggggactct acaggcagcg tttctgtaat gctatgccgt taagcgggtt gcgccgtaag
    20041 ccattatccg ggatgggttt aggcgttttt atgcttttgt atcgagttct gtcggacgcg
    20101 gaggttttga ccctcacgtg ggcacaaaat ggacacaagc acctcagtcg cgcggcccgg
    20161 ccgacggtct aaggatgccg acatcgcgca cgcatcgtgt cccaccgcat tcatcgccgc
    20221 tggttcttcc tttcgagctt gaaatgaacc ttgtacgtgt agaagtcaat gttcggagcg
    20281 acggccagtg cccgacggag ctttatgtcg tcttcgaagg cgatgataac cccgcgtacg
    20341 ttctggtttg attccgccag ctcctccttc acgtatccca tgtaccgctg cacctgcccg
    20401 accaccacgt cgctcgccct gcccttcttg agttcaatga cgacaatttc ctttccatcc
    20461 ttgctgaccg ccagaatgtc aatcggtccg gtgtcggtct ggtactgctg ccctgccaac
    20521 tccccgtcct cctcgaatac gtcgtagtgc ttaccgagct ccgtatgatg ccagttctgt
    20581 acaaggaagt cttccaggtg tttctccaac gcgaaagcgc tcggatcttc aacggtcgcg
    20641 tcagtactga caatggtcag tggttggccc ccggcgatga gccgctcgat ctcggcggca
    20701 tgccgggaga cgttgatgac cggagggccc gacgaaagcg attttcggag cggctcactc
    20761 atctcgtcac gggtcaaggg ccggtcgaac cagcgcacca cgcggcggtg cggcagttct
    20821 tctcccttct tgtactcata tccgcccgtc acctcaccga cgaagtaatt ccccttgccg
    20881 tcggggcaga gtacgacatc ccctacctga acgcccttgg ccacggtcca cagcatgccg
    20941 caagccaggc cggccgatac cttgggcttg ccagggtttt gcttcatcca gacagggatg
    21001 aactctctgt tgaaatcccg ccagttgtcg ggcaaatgat gtgtcaggtc tcggtcggtg
    21061 atgaatccgg ccgcgatgca gttgctcctg tacgcctctt cagcaaaaca gttcttactg
    21121 ccaagcagaa tgcggaaata gcttttcatg atgcggattt gagcctcatt ccatttatgt
    21181 gctcttgcgc gatacccaga actctcgctg ttgtgctacc gctcgattat gaccacgcca
    21241 caaattaacg agcactgtac aatgaggcaa ccgctttctc agactccgcg ctctgacgct
    21301 ggccgctcct tagctcgatt cggccccgcc acggggagga gaaacgtggg tggtagtcgc
    21361 acactacgga ttccttatgc tctcgcgtag aatgaaacgc acgaaccgcc cttcgcagct
    21421 gatcgacgac acgggtccca tccatctcgg ctgccagccg cccgtcgcgt ttgacaaaga
    21481 caaggcccgt gaccgaagcc acgagcctac atatccgcat ctcaccgcca actcacgggc
    21541 acgctaccgg agccgggcca ccgcggaaca cgtaggcgat gaggaaagtg atatccgcaa
    21601 cagccaactt tccatccgcc gtcacatccg cctcggcaag gcagtccggt gcaggcccgc
    21661 cgcgaaagac atggttgatg aggaacgtga tatccacgac cgtgaccttg tcctcttcat
    21721 cgaaatcaac attccctctg ataccgttgc agcacttggt ccactcagcg atttaggcgg
    21781 agaccaggct gcctgcgacg gtgaagcggc cgcctgcgat cagggtgcgg tcgaacacgg
    21841 tcagggcatt gacccagtca ttcattcccg agccaagcgg cgaccaggtg gaaccgtccg
    21901 aagcggcaat gtaattggca ctggtcccgc ctgccgcggt gaaatagccg cctgcgatca
    21961 aagcgccgtt gaacacggtc agggcgttga cggcatcatt cattcccggc ccgagcgcag
    22021 ggaatccgtc ccccgcggcc agcacccacg ctttcggtcc ccgtcgcggt cggacacgac
    22081 tgccacgccc gtgctgctgg tcggcagtac tcgaggtcac atcttggccg cctacacaaa
    22141 gaaggccaga tcctcgctgc cctgtgcgac tacctattgg tcaaccttca tgagctgctc
    22201 gaccttgtgc tggagttcgt caaggcgctg acgaattgcg gagttgtccg cgttcaacga
    22261 cgagttctcc tttgccaact gcttgaccgc ttccaccaag acggcggtga gccgaccata
    22321 gtcgacggcg cggtaattct ggtcgcccca ggtcgtaacc aactccggaa gtactttctc
    22381 aacctcctgt gcgatgacgc cgatttcccg atgaccggag gaccgtccga gggagtcgta
    22441 tcgctcattc caatcgaaag aaacgccgtt tatctgctcg atcttctcaa gggcgtcgac
    22501 aattggcgcc acattcgcct tgagacggtc gtccgacgaa gtggggaagc tggtcgcgtg
    22561 gcatgagccc gcaacatcga gcttgtagcc gggtgtggag gtaccgacac cgacgttgag
    22621 attgggtaag agagcgatat caccgacgtc ggtggccaga tagccgtcgg tgccattgtg
    22681 gtacatctct atccgactgc cccagcccga cggatttctc ccgtagaccg caaggtgcgc
    22741 gtcatacgat ctgttacccg tgaatccgga gtagaagccg aagtactgat cctcagtgtc
    22801 gtctccttcc aacgtcccaa gctgattatt gctgaaaaag atgccgcagc ggccgggatc
    22861 attgtgactg tcggtgtgaa gtgtcaatcc tccgatgtgt tctgagaagc gggtgatctt
    22921 gacgttacca gtgaagaatc cggccgaatc accgaagccg cagaccgcct tgccgtaata
    22981 agccagtgcg ccggagcagt ctgcccaatg acctgagccg gtatcagtct gggcaagaac
    23041 tccagtacgg ttcgacgcga tagtgactat tgcgcctccg gaatcattta gcgccaataa
    23101 cccctcgtcg tactttccta tcgcttcagc aagaatcgcg cgtgtatcct cgccaagcgt
    23161 cgcgtagttg ccgctactgc tatgctctcc cataatccca taggtggagc ctttcccata
    23221 gactccgcgc cgcccgagcc cgtacactcc tagaccgaac gcacttgaag cgtatacccc
    23281 tgtgctgtct gtactggaag cgcgaatgcc gaccccggaa gtacactcgc cgaatatacc
    23341 aacttcgttt gcctgctggg ctgaacctgc aacgccaaaa tactcacctc ccagcgttcc
    23401 actgcaaccg gaactggtgt cctttccgaa cacgccgtca ttcaggcctc ccagataacc
    23461 gtagttgtgc gaggtcggat gttcaccgaa cactcccttg ctccccgtcc cgtagatacc
    23521 atacttagac gcactggagc cttgcacgcc atagcccgaa ccgcaataac caaagactcc
    23581 agctgaactg ccgctcactg cactgccgga aatcccgtgc cccgatccac tggttgcact
    23641 gtagagtgca ggagttgggt tggcggcgtt gtcgaggttg aagtatgcgg tgccgcccgt
    23701 tgcatcggtc atggtgacat cgagtttgtt gttcgctcca ttcgtaccta tcgagacctt
    23761 gtcggtgagc gtcgtcagcc ggaccgtagg tccgccgtcg gtccacccgc cgccaccgcc
    23821 ggcgactccg aaggccatgg ctgccgtatc ggcgaatagc gccttaaagg cgaaggggac
    23881 ggtgttcagt ttcacgcggg ggatgatttc cgggtccgcg cctactttga tgccaaggta
    23941 ccgcgtggtg tcgccggtga agagtgtgct cggcagggcc gtggaatccc cgagagtata
    24001 ggcaaacaac cccccgatta cggtgacgtg tcggtagccg ttgtcccaga gaaccacccc
    24061 ctcggttgga gcgttgtaga tgacgaagcg aatcagataa ctgccgtcgg gaacattggc
    24121 gccggccgat gtggttagcc gtccctggta agtgaacgtg gttggaacgg cggcgagcgc
    24181 cagcgctgcg gcgcacgcca caatgagcat ggccgaaatg agtttggata tgagatgaag
    24241 tcgcttcatg gcggattctc cttggctgtt gatgatcata tcttccctgc gagtaatgga
    24301 acgttccgac aggaaccgaa ctgttcgctg tcgatgattc gatatgaata agatagcccc
    24361 atttgcatat ttgtcaacgc tgtattgaac ggggtttggc tgctccgagc cggcagtgca
    24421 gacgggatgc tcacgcgtga ccgccgctgg gggggagcgg cgtggggggt agttcgcagg
    24481 ctatggattc gttgctgtcg ggcgcttgac gaaacgcaca acccccaact cacttctaat
    24541 gcacggcacc ggtcccgagc agctttcacc tcggttagcg gccgcgttcg acaaagagaa
    24601 ggcccgtgac cgaagtcacg ggcctacata tatgcatctc ataggtcaac tcacgggcag
    24661 gctaccggcg gcggcccgcc tctgaaaacg taggcaatca gaaacgtgac gtcagcgacg
    24721 ttcacgtacg ttgggtcatc acctgtgacg tcagcatgaa ggctgcaatt cggaggtggg
//
LOCUS       DAOPOE010000046        24723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2555_length_24723_cov_5.603332, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000046 DAOPOE010000000
VERSION     DAOPOE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24723
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_24723_cov_5.603332"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKM94_07605"
     CDS             complement(<1..509)
                     /locus_tag="PKM94_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66546.1"
                     /translation="MYNLLRRLVFAAALVATAPLIFITWLEGVVTSRRSEKLYGSCKE
                     LLASVPTFFGEYFRGAFYWAVCAHVETGVVFLYGSMVAHRDTTVRRGAIIGCHSIVGY
                     ADIGPDVMLAARVSILSGKYQHGRPGELRGAGVEGSYSRVTIGERSWIGEGALVLANV
                     GKDCTVGAG"
     gene            complement(535..1629)
                     /locus_tag="PKM94_07610"
     CDS             complement(535..1629)
                     /locus_tag="PKM94_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66547.1"
                     /translation="MRKRLILLTLAVLLTAGTLAARTQRVSVIFIHYSTGTIIVEGYC
                     WGNQWNRNITEALDTTVVSYGIDTARIVFHSYRLNNDAGFSALSDTCHGSGENGCAFN
                     RFSNFSYDLVTSSYNRMRIWNSDGGFTSGSYAGLLKHFFDVPNKEQQTFWKLFKTHKT
                     PSQFPDSVAEEDGYDLVIIKNPYACWAYMTPAQADSIRVLYRAVRDSIAHHPEINVAL
                     AFGTPLRLGTQVFDSAQAKITYDLATWFVSDSFFTHTNDGPYRNLWKVDTYRPYCEMS
                     PDSVNRYCLKNAYYGDGDSHPNPQAGATLGQDILIDFLRTAVRDILIQRSGAGGVTRA
                     DIDRKIRDFQQGLASEEDVLTLIRQYNSAR"
     gene            complement(1837..2949)
                     /locus_tag="PKM94_07615"
     CDS             complement(1837..2949)
                     /locus_tag="PKM94_07615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66548.1"
                     /translation="MRLEIITSLAAFRDLNGPWNDLLRGHPLDHAFMRHEWFACWLAH
                     FSGSSALCCAVARDRDRLVAAAPMLVSRERIKGLSARVLGFAASTITPRCNVILAPDC
                     AAEEFFAFLLAIPGVDLVVARGLDREAAATAAFTGFLSQTGRAAVSEPGRLSPFITTG
                     GAWGDYFRSLPKSFRQNLRTGANKLGRAGDVSIKKVDTYVEFKHLLPELVNVSARSWK
                     AAEGTDLGSLPQVVAFLDDFSRLGEPAGLWELWVLFLDGRPIAFDYYLCGPQRLSLIR
                     TDFDLAFKDLSPGHNLQQAVLQDLFARPGVWEHDMGGQAYGYKLRWTDKLRPHIDLWT
                     AGSRRWGRVLMFGKSRFLPLIRRPSRRSESETGETA"
     gene            3136..4323
                     /locus_tag="PKM94_07620"
     CDS             3136..4323
                     /locus_tag="PKM94_07620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66549.1"
                     /translation="MKETTDPAAPAGTPGVRVAVYRRIEELEPLAAAWDRLVFADTGR
                     LPMSSHAWVASYLAERHPAARRWMCLAAFAGHRLEGVWPIEVEEIRILGRRLPMLRQP
                     WDSHMQSVEAVVRPGRERAVLTALRAALDDAAPAWFGLNFDHLEEGAPLAGEAARALT
                     RTTLLCEGSNYASVVKCTGDWDEYLAGLGRNFRKHLRKEHRRLAEIGEVRTEFLAGPQ
                     ADPELLRRFARIEAAGWKGKRGTAISSRENLTAFYRALCRRLAERGALEWHFLRAGGH
                     DLAGHFAIRTGKALVIQKIGYDEAFAAYSPGVVLMERALERAFADAATAEVNLVTDRA
                     WHDAWHPEKRPYIQVRMYPRRVRSLVAGAWPHRVRLALRRVRPLRRAVGWWRSRRTGP
                     GAD"
     gene            4474..5655
                     /locus_tag="PKM94_07625"
     CDS             4474..5655
                     /locus_tag="PKM94_07625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66550.1"
                     /translation="MTNEVLVSRRPAAPHLEVRVYRDPAELAAIGEAWDELALGAAGR
                     LPMLSYAWVSAHLELRQPAARPWLCLAAFAGQQLAGVLPLEERRLSVLGKTVTALRPP
                     GDAHTRSVDAVVAPGREAEAAEAFYRALEEAVPGWFGLEMPGVEGTSPLIAAAAAGAP
                     ARARAAQIPAGLATVRETQGTWDEFFESSSGHFRKHYRQYGRKLAKLGTVEAEFLSGA
                     QAHPEYLERFARLEAAGWKGRAGSAIGADPALTEFYRRLCARLHARRALEWHFLRVGD
                     TYVAGHLAVRTGRTLVLWKIGYDEAYAACSPGFLLIGEMLQRAFADPATDAVNFVTDR
                     TWLDSWRPEKHRYVDLWLYPRRLRSLVGGWWPRQARQRLRETPGVKPLLRALRGKPEP
                     Q"
     gene            5728..6753
                     /locus_tag="PKM94_07630"
     CDS             5728..6753
                     /locus_tag="PKM94_07630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOD66551.1"
                     /translation="MRQPVSVIIPTYNRAAMLPRALESVLDQLTAQDEVVVVDDGSVD
                     RTAAVIEEIRAKRGSTNGTIRYLFQDNAGAGAARNRGIREAAHDLVAFVDSDDEWLPG
                     KASMQCALMAARPELVVCFGDFKLRYADGRVIDRGMVRWHQDARTWEERLGAPMNLSE
                     LAEWPKEQDVRVYMGDLYAAHMRACYLSAFTMMVRRSRAGAGFYFTEGIRMYEDWGCF
                     GRLTEAGPAGFLDCDLAIQNGHREGRLTDADAFVRAVSRAVVLEEVWGADPEFLRTHG
                     EEYRALLDQQRVVKAAGYLSQGQPAAARAQLAQVRKAPLSYRLLAMLPAPAVRGLLAL
                     RRAIRRG"
     gene            6861..7703
                     /gene="xrt"
                     /locus_tag="PKM94_07635"
     CDS             6861..7703
                     /gene="xrt"
                     /locus_tag="PKM94_07635"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HOD66552.1"
                     /translation="MTTATQVRSYVGRKPILGIGLLFVLVFLPVLIDLVTRWWEDSNY
                     SHGFLVPLIAGWVVWRKREEIGGLPAEGSRAGLAVLAAGMLLFVLGNAGVEYFVVAVA
                     MVTVLFGLTLYLFGAEVIRRTWFAFLILLFMIPIPGVIYYSLTFPMQLLASKVSVAAM
                     HALGMTVVRQGNVILLPGQALEVAEACSGMRSLISLMAMGALYGYLSQRRFWAQVLLF
                     ALTVPIAVVSNVVRVFVTAAVAYAGGIDPTAEPLHTLFGLSVFVVAFVLLTIAAVVLR
                     RWQR"
     gene            7700..8332
                     /locus_tag="PKM94_07640"
     CDS             7700..8332
                     /locus_tag="PKM94_07640"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOD66553.1"
                     /translation="MTNRVIIAAILLAAGGALGDFFRFVGRTPGRPADLEGIPAAAAD
                     YQGREEPFDAATYEVLNATSATLRQYAGADGSMAELFVAYFASQRFGAGIHSPRHCLP
                     GGGWQIVRHEAAALEAPGGPARTVNRLLIEFEGRQSLMLYWYQTRSGVVRGEYGLKLD
                     LLRNALAMRPTDAALVRVTVPVAGDEGEAMARAVQFARTIDPYVQQALPF"
     gene            complement(8352..9173)
                     /locus_tag="PKM94_07645"
     CDS             complement(8352..9173)
                     /locus_tag="PKM94_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66554.1"
                     /translation="MSKPLFVVLGLLAAAPAAAQEPVYPAGAGVASTYTLSDTVLALG
                     DTLAVTRTLVNGGSSPLAGLYFSENLPPDFTVVGESATVNGIPVAVLREGPLAGEVIP
                     GYAAYRWIIDSPDRAVTRTLSPGDRAELTYRIVSSDIGRFPLPLHDAAGCGDSDGVFA
                     VSSGDTVEILLSLGVDDDPGYLLPANALQTIAFPNPFNAEVSIAFHNAVAGGGALQFD
                     VYDILGRRVDRQVIRPEGSEGIVRWEPDPSVGSGVFLYRLTDARHTAAGKVLLLK"
     gene            complement(9231..9497)
                     /locus_tag="PKM94_07650"
     CDS             complement(9231..9497)
                     /locus_tag="PKM94_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD66555.1"
                     /translation="MSVRTELRQFIRDNFMIGRDPEELTDSGSLLEMGIIDSTGVLEL
                     VAFLEERYGIHVDDTELVPDNLDSLNNLEAYISRKQASAPTSGA"
     gene            9814..10206
                     /locus_tag="PKM94_07655"
     CDS             9814..10206
                     /locus_tag="PKM94_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66556.1"
                     /translation="MREKPTTLPELLQAALRSQVELGLGEMIIDPARVETLAAEAARG
                     AANQREEWVDRMDAAVRRTSPPPVDLFGGAGGTGPKAAQFGTLAEHRAAICECRNCPL
                     GRDAHEIRLRRGESAGGADVRGGGAGAG"
     gene            10175..10630
                     /locus_tag="PKM94_07660"
     CDS             10175..10630
                     /locus_tag="PKM94_07660"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOD66557.1"
                     /translation="MFVGEAPGRDEDLKGEPFVGRAGQLLDKILAAMSMARAEVYIAN
                     VLKCRPPDNRDPLPEEMAECLPYLKEQIRIIRPKVLCALGRIAGQALLETKTPLGQLR
                     KRWHEYEGVPLLVTYHPAALLRFPEYKKETWEDMQMVLAKLDELRSRAG"
     gene            10656..12089
                     /gene="dnaB"
                     /locus_tag="PKM94_07665"
     CDS             10656..12089
                     /gene="dnaB"
                     /locus_tag="PKM94_07665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOD66558.1"
                     /translation="MAYRSQEEKREEKLQPPQSLDAEQAVLGAILKDSDAMNQVVEVL
                     DDESHFYAARHQAIFRAAKALYERGEPTDITMVANELLRNDELGRIGGRVYLVELVEG
                     VASTANLSAHATVVLERSILRRLITTSNEIIKSCYAFEQPVAGLLDRAESTIFRLSES
                     RLRKGFTSIRDLIPSTFEDIEALQSNESGLTGLPTGYEELDLMTNGMHKGDLVIVAGR
                     PSMGKSALAMNIAEHVAIKQRKGVGVFSIEMSKEQLALRMLCGRAGISQQKLRAGKLR
                     DTEWSRLATVGGILSEAPVFIDDSPTITSLEIRAKARRLKAQQDVGVIIVDYLQMVHG
                     SGRFENRQQEMATISQGLKALAKELEIPVIACSQLSRLVEQRGGEKRPQLSDLRESGA
                     IEQDADLVLFIYRPEHYLTHLEQNDPKMQEVAGLAEIIVAKQRNGPTGVVKLHFRKEF
                     ARFENLERHHREIPPGVEPVAGGDAPF"
     gene            12099..12878
                     /locus_tag="PKM94_07670"
     CDS             12099..12878
                     /locus_tag="PKM94_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66559.1"
                     /translation="MNLGVTTLGRQGVNAIDEVGGMAILFGRFLVALSGLPRSFRLYV
                     EQVYSLGVRSLPLIVIISVFVGAVSAWQGAYQFSFIGAPLRFLGQAVGKAVVIELAPV
                     LSAIVFAGRVGAGVAAELGTMRVTEQIDALESMGIDPVRFLVMPRILACLTMVPFLVV
                     FANFIAILGGMGVAVLGVDISAEMFLDGFHSSFSLSDFINGEIKAAVFGLLIGLVGCY
                     EGFNTKGGAAGVGEATTTSVVISSVLILVSNFLFAIVLFRF"
     gene            12882..13658
                     /locus_tag="PKM94_07675"
     CDS             12882..13658
                     /locus_tag="PKM94_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66560.1"
                     /translation="MDTLRPQAESVCFEVEDLHKAFGAQRVLDGVSLKVQTGESVAII
                     GQSGSGKSVLLKHLIRLLVPDRGRVLYRGRDLADMSSGELVEVRRKIGMLFQSAALFD
                     SMTVAENVGLGLREARRHEQREIDRIVAEKLEMVGLAEAGDKYPAELSGGMRKRVGLA
                     RAIANDPDVLLYDEPTTGLDPVTADVINDLIVNLNRQLRVTSVVVTHDMKSAFRIAGR
                     VVMLYDARVRFDGSPQELQRAGDPVLRQFIAGDSAGPIRI"
     gene            13655..15052
                     /gene="radA"
                     /locus_tag="PKM94_07680"
     CDS             13655..15052
                     /gene="radA"
                     /locus_tag="PKM94_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOD66561.1"
                     /translation="MTGSRAAMADKRSGKVKTAFFCTQCGAEHTRWQGQCRECAEWNT
                     LVEERLVKPAGGKGRGTRPPAEARRLPEISLAAEAGIESGIGEFDRVLGGRLLPGMSV
                     LIGGEPGIGKSTLLLQAAEAYSRRGHRVAYVTGEESLAQIRLRAERLGVAGENIVAVN
                     ANALEEICDLVAEGAYEIVLVDSIQTVASAMFDSPPGTVAQVRESAQRLMGLARQHGF
                     ALFLVGHVTKDGMVAGPKVLEHMVDTVISFEGDGSHLYRMLRVMKNRFGSVAEIGLFE
                     MQSRGLVEVTNPSSLFLSQRQDARRTGSVVAGICEGQRPLLVEIQALVTAASYGNPQR
                     VAGGIDNKRLALLIAILEKKGGFPMGMNDVFVSIAGGLRLSEPALDLAMLAAIASSLL
                     NKPVDPRTMVVGEVGLSGEVRGVTMIDRRLAEAAKLGFTAAVIPAMNAAQAKRELPSV
                     HGVGSLQEAIEVLLG"
     gene            15049..16164
                     /gene="tgt"
                     /locus_tag="PKM94_07685"
     CDS             15049..16164
                     /gene="tgt"
                     /locus_tag="PKM94_07685"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOD66562.1"
                     /translation="MIRILCRDGAARRGEAATAHGLFQTPAFMPVGTAGAVKALTCED
                     LEEIGVEIILGNTYHLYLRPGPETIRESGGLAAFSGWGKPTLTDSGGYQVFSLKDRLR
                     ISDDGVEFRSHHDGSTHLFTPEKVIQIQHAIGADIIMAFDQCVPYPAERRAVEEGVRR
                     TGEWAARCAREHERRSESGPDPGRLFGIVQGGTYADFRTRSAEQILALDLPGNAIGGL
                     SVGESKAEMEAMLAHTVPLLPADKPRYLMGVGYPEDILTAVSYGVDMFDCVLPTRNAR
                     TGNVFTTVGPITYRNAAYAQDQRPLDPHCGCRVCRRYTRAYLRHLYNQSEITGIVLAS
                     YHSVYFFQELMRGIRRAIEERRFDAFRREFLARYAGE"
     gene            complement(16350..17078)
                     /locus_tag="PKM94_07690"
     CDS             complement(16350..17078)
                     /locus_tag="PKM94_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOD66563.1"
                     /translation="MERYRRQLPVDRWNQDRLKSARVLVVGAGGLGGISATCLAAAGV
                     GHLCICDGDRVELSNLNRQFLFAAGDIGKPKAVLAAEKLAAQNPEITVEAIPLKLTED
                     NAGELASGCDVIIDGLDNHAGRLILNDVSFRLSIPYVYGAIHEWLGQLSFLNPPKTAC
                     LACLLHKDALHAGPTPVFGALPGVIGSLQATLALRYLMTGDMPVSNTLLIFRADTMAF
                     EMVGFEKRPDCRVCGKPVPPDPRP"
     gene            complement(17112..17351)
                     /locus_tag="PKM94_07695"
     CDS             complement(17112..17351)
                     /locus_tag="PKM94_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66564.1"
                     /translation="MIVFLRAGGQLRAMLRPDVDHYTRRVQIDGDRTIGEILEAISLD
                     PAYVAVIYVDGHVKDLSFKPSNGQTITLQPPVSGG"
     gene            complement(17507..19444)
                     /locus_tag="PKM94_07700"
     CDS             complement(17507..19444)
                     /locus_tag="PKM94_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOD66565.1"
                     /translation="MAHESASCGGMAGTTLRINLTTGEIRRHPTDAKLVREWFGGRGT
                     IARILYDEVPRDADPFGPENLFIMNAGIMSGCFIPAGAKVEFGSISPLTNGHGDSNMG
                     GHLGPALKFAGYDMVIMSGISPVPVYLFIDDDTVELRDASPYWGMGSLECEPAMKKDL
                     GEDFEIATIGPAGENGIRFSCITHDFGRQAGRTGQGAVMGSKKLKAIAVRGTRGIKVH
                     DLDRLTEHVSAIIKRTQTHPNMAPWQKYGTAFFIDWSNVNAVLPTRNFQTTYYENSKG
                     IDGDALVEKCLITHKACFGCWMNCGKYTKAVVPGKETVYLEGPEYETGALCGSNLGMT
                     DINQVAYLNWLMDNVGMDTMSGGGVVAFGMECFQRGLISADDLEGHTLNWGSVDDAEH
                     FIDMIVHRRGIGDYFADGTRRAAEKIGRGSEKFAMQVKGQEMSGYDGRYAPGMLLSYM
                     TSDIGAHHNRSWTITVDMAEGRDRIEGRAKVVVYLQHIRPLFDCWCICRLFWGELDVE
                     PEEIVVALNYITGWNVDTAEALKTSEKIWNLVRCHYLERNRANGRTFDYPPARSWEDK
                     IPTGPSAGKGLTRDQIEAMLDEYYAARGWDADGNPSREVLHDLKLHFAADNIEKIGLL
                     GKPLPNGIPAVRGERYKPKAF"
     gene            complement(19444..19860)
                     /locus_tag="PKM94_07705"
     CDS             complement(19444..19860)
                     /locus_tag="PKM94_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD66566.1"
                     /translation="METKRLKVSPGKCSGCRLCMQICAISHYHEINPRKASLRIEVLF
                     PQPGLYKPKVCTQCGRCLEACPEHAIFRRDDGAYIVIAEKCTNCGDCIAVCKPGVIFQ
                     HRDVDHVIICDNCFQCAELCNTGAITVFTRPAKEGK"
     gene            complement(19878..20519)
                     /locus_tag="PKM94_07710"
     CDS             complement(19878..20519)
                     /locus_tag="PKM94_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66567.1"
                     /translation="MRRYEKILVLLVLGSLWGALELFGRDLLRAAGAPEKSAVLFGLG
                     IIILYASKRLVDFPGSVVVMALIAGLFKTASSYFFPCQFAAVMINGIVFDIAYSAFRE
                     RLDSSPLYRVLAAPVIVYASYAAFALIAAYVLREASWAANGWAGIRSFLASDAVSASL
                     ISIVTINLGYYLGNAIQPYALLRKWRAPAVLFRVVSVVLVAAIWIAGQVYAKA"
     gene            complement(20911..22659)
                     /locus_tag="PKM94_07715"
     CDS             complement(20911..22659)
                     /locus_tag="PKM94_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD66568.1"
                     /translation="MKTTAIFGLVLIALGSPAPAQTPCDDLSDAPVRFAVLGDRTGDH
                     QDGVYESVVAEIERLRPDFVVTVGDMIEGHTTDTIEMRSRWDEYFAIIRPLTMPVRFT
                     PGNDDISTDAMESTYRARAGEPYYAFDHRGIHFVVLDNSRTESAGEIAQEQLGWLKDD
                     LAKNRSACYTIVFLHKPFWYRTLGSGQPDALHDIFRANGVDAVFTGHMHCYFSAEFDG
                     ILYTSLGSSGAETEESPDGLRYHFGWVTVDDRGVHIAPIKKDAVLPWDVQTVAEARAA
                     SRVRSIGIRFAQPLPLTDGLKPAAGAVTVLLDGPTAGGQWTDTLRWDVPEGWTITPAA
                     CPFTLVPGATVTAQFAIEPAPALLPLPVISTRLPYAAEKSTRVSRELEVARAARCIRV
                     NEPVVIDGQLIEPCWARPVTALFSKNGTPAAQDPTMFYFAYDSDNLYLAAHCRDSDMA
                     AIHAAMTERDAPVFTEDAVGWMIELGGTNRVVAQVYVNALGTLYDQQVQQASDGYWAG
                     RSDWNGDFEVKTVRGADFYAVEMRIPLAQFAATPDSGDRWRLNFRRRQVRMNSSASFQ
                     TSWSYDPAAFGELVFE"
     gene            complement(22894..23502)
                     /locus_tag="PKM94_07720"
     CDS             complement(22894..23502)
                     /locus_tag="PKM94_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66569.1"
                     /translation="MLTILIAAWLVSPLYPLNPVVEDTVNGVNMHFTIGVGGPNVLTD
                     PGLLISAKYEFLLRHPFLLRACADYGFAAMRSKTYPDGALHSMILSAEILLYRGTDAM
                     TGFVGMGPLLYKGYFRFDGLEPTDFVPGAVGLDIAMAPKIGYRATLGLRFNRNFSLEI
                     GMSEARPTIRITGDTGDSRLRTFSQPVRLHDVRMSVGYLVPL"
     gene            23863..>24723
                     /locus_tag="PKM94_07725"
     CDS             23863..>24723
                     /locus_tag="PKM94_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOD66570.1"
                     /translation="MSEPRTFLNLIKGEWVRSKSGKTYDNINPANTDEVVGVFQKSNA
                     EDVKDAIDAAAEAYKSWRLVPAPKRGDILFKVAARLQKEKENLARDMTREMGKILDEA
                     RGDVQEAIDMAFYMAGEGRRLFGQTTPSELRNKFNMSIRQPLGVCAFITPWNFPMAIP
                     SWKIMPALVAGNTVVIKPATDTPLSVVNLVRIMHEEGVPAGVVNVVTGSGGALGEPLI
                     RDPRVRVVSFTGSTEVGRKIAEMCAPQFKHTCLEMGGKNVQIVMDDAKLELAVEGALW
                     GAFGTTGQRCT"
BASE COUNT         4248 a         7200 c         9077 g         4198 t
ORIGIN      
        1 ctgccggcgc cgacggtgca gtctttgccg acgttggcga ggacgagggc gccctcgccg
       61 atccaggaac gctcgccgat cgtgacccgg ctgtagctgc cctcgacgcc ggcgccgcgg
      121 agttcgccgg gtcggccgtg ctggtacttg cccgagagga tcgagacgcg ggcggcgagc
      181 atgacgtcgg gcccgatgtc ggcgtagccg acgatcgagt ggcagccgat gatcgcgccc
      241 ctacgcaccg tcgtgtcgcg gtgggcgacc atgctcccgt agaggaacac gacgccggtc
      301 tcgacgtggg cgcagaccgc ccagtagaat gcgccgcgga agtactcgcc gaagaaggtg
      361 gggaccgagg cgagcagctc tttgcacgag ccgtagagct tctcgctccg ccgcgaggtg
      421 acgacgccct ccagccaggt gatgaaaatg aggggcgcgg tcgcgacgag cgcggcggcg
      481 aacaccaggc gacggaggag attgtacacg gcggttcccg gaactcctgc gccctcagcg
      541 ggcgctgtta tactgccgga tgagggtgag cacgtcttcc tcgctggcga gaccctgctg
      601 gaagtcgcgg attttgcggt cgatatcggc gcgcgtgacc ccgcccgcgc cgctgcgctg
      661 gatgaggatg tcgcggacgg cggtgcggag gaagtcgatg aggatgtcct ggccgagcgt
      721 ggcccccgcc tgcgggttcg ggtgcgaatc gccgtcccca tagtacgcgt tcttcaagca
      781 gtagcggttg acgctgtcgg gactcatctc gcagtagggg cggtaggtgt cgactttcca
      841 gaggttccgg tagggaccgt cattggtgtg ggtgaagaag gagtcgctga cgaaccaggt
      901 ggcgaggtca tacgtgattt tggcctgggc ggagtcaaac acctgcgtgc cgagtcgcag
      961 tggcgtgccg aacgccaggg cgacgttgat ctcggggtgg tgggcgatgg agtcgcggac
     1021 ggcgcgatag agcacgcgaa tgctgtcggc ctgtgccggc gtcatgtacg cccagcaggc
     1081 ataggggttc ttgatgatga ccaggtcgta gccgtcctcc tcggccaccg agtcggggaa
     1141 ctgcgaggga gtcttgtggg tcttgaagag cttccagaag gtctgctgct ccttgttggg
     1201 aacgtcgaag aagtgcttga ggaggccggc gtaggagccg ctcgtgaagc cgccgtcgct
     1261 gttccagatg cgcatgcggt tgtaggagct ggtgacgaga tcgtaggaga agttggagaa
     1321 gcggttgaag gcgcagccgt tctcgccgct gccgtggcag gtgtcgctca gcgcgctgaa
     1381 gccggcatcg ttgttgaggc ggtagctgtg gaagacgatg cgggcagtgt cgatgccgta
     1441 ggagacaacg gtggtgtcga gcgcctcggt gatgttgcgg ttccactggt tgccccagca
     1501 gtagccctcg acaatgatgg tgccggtgga gtagtggata aagatgaccg agacgcgctg
     1561 ggtgcgcgcc gcgagcgtcc ccgcggtaag gaggacggcc agtgtgagga gaatcaatct
     1621 tttccgcatc tgagcattcc ttgtgaggtc aggccgcact cgccgagcct gccgacatat
     1681 aatgtcgaac ggggcgacga ggcaaccgta aagtggagga ggaccggtta cgtttcgcaa
     1741 gcaccgtacc cgcaacaagg tacgggcgta acgccgcgcc ccctccggtt gttccttttg
     1801 gacataatgt cggcggaaca ggcgggcaac cttacctcaa gcggtctccc cggtctcgct
     1861 ctccgagcgg cgggaagggc ggcgtatgag ggggaggaag cggcttttac cgaacatcag
     1921 cacgcgaccc cagcggcggg agccggcggt ccataagtct atgtgcgggc gcaacttgtc
     1981 ggtccaccgg agcttgtacc cgtacgcctg gccgcccatg tcgtgctccc atacccccgg
     2041 gcgggcaaac aagtcctgca gcaccgcctg ctggagattg tggccggggc tcagatcctt
     2101 gaaggcgagg tcgaaatcgg tgcgaatcag cgagaggcgc tgcgggccgc agaggtagta
     2161 gtcgaaggca atggggcggc catcgagaaa aagcacccag agctcccaca acccggccgg
     2221 ttcgcccagg cggctgaagt catcgagaaa agccaccacc tgcggcagcg aaccgagatc
     2281 ggtgccctcg gcggctttcc agctgcgggc ggagacgttg acaagttcag gaaggagatg
     2341 cttgaattcg acgtacgtgt cgactttctt tatactgacg tcgcccgcgc ggccgagttt
     2401 gttggcgccc gtacggaggt tctggcggaa agatttgggc agagaccgga agtagtcccc
     2461 ccacgcgccg ccggtggtga tgaacgggga gaggcgtccg ggttcgctca cggcggcgcg
     2521 cccggtttgg ctgagaaacc cggtgaaagc ggcggtggcg gcggcctcgc gatccagccc
     2581 gcgggcaacg acgagatcga cgccggggat agccaggaga aaagcgaaga actcttcggc
     2641 ggcgcagtcg ggagcaagga tgacgttgca gcgcggggtg atcgtggagg cggcgaagcc
     2701 gaggacgcgg gcggaaaggc ccttgatgcg ttcgcgcgaa accagcatcg gggcggcggc
     2761 gacgaggcgg tcgcggtcgc gggcgacggc gcagcagaga gccgacgagc cgctaaagtg
     2821 cgccagccag caggcgaacc actcgtggcg catgaaggca tggtcgagcg ggtggccgcg
     2881 aagcaggtcg ttccacgggc cgttcaggtc ccggaaggca gcgagcgaag tgatgatctc
     2941 caggcgcata gtcattcaca cagcgcgtgc gttagcggcg gaccgggcat tcggtcctcc
     3001 ggccgcaact aatgcggatt ggaaactcag ggcaagcaga gaaacgcgga gcgaatcggg
     3061 cggcggagca gcggcgggcg ccgcacaccg cttccggccg ggccgacccg gccgcgaccg
     3121 agggaagggc gggtcatgaa ggaaacaaca gacccggctg caccggccgg gacgccgggc
     3181 gtgcgggtcg cggtgtaccg gcggatcgag gagctagaac cgctggcggc cgcctgggac
     3241 cgcctcgtgt tcgccgacac cgggcgcctg cctatgagtt cgcacgcctg ggtggcctcg
     3301 tatctggcgg agcggcatcc ggccgcccgc cgctggatgt gcctggcggc gttcgcggga
     3361 caccgtttgg agggagtgtg gccgatcgaa gtcgaagaaa ttcggatcct ggggaggcgc
     3421 ctgccgatgc tgcggcagcc ctgggattcg cacatgcagt cggtggaagc ggtggtccgg
     3481 ccgggaaggg agcgggcggt gctcaccgcg ctgcgggcgg cgctcgatga cgccgcgccg
     3541 gcgtggttcg gactgaattt cgaccacctc gaggagggtg cgccgctggc cggagaggcg
     3601 gcgcgggcgt tgacccggac gacgctgctg tgcgagggga gcaactatgc ctcggtggtg
     3661 aagtgcaccg gggactggga cgaatacttg gcagggctgg ggcggaattt ccgcaaacac
     3721 ctccgcaagg agcatcgccg gctggcggag atcggggagg tgcggacgga attcctggcc
     3781 gggccgcagg ccgatccgga gttgctgcgg cggttcgcgc gcatagaggc ggccggatgg
     3841 aaagggaaac ggggaacggc aatctcctcc cgggagaacc tgaccgcatt ctaccgcgcg
     3901 ctgtgccggc ggctggcgga gcgcggcgcg ctggagtggc attttctgag ggcggggggc
     3961 cacgatctgg cgggacattt cgcgattcgg acagggaagg cgctcgtgat tcagaagatc
     4021 ggctacgatg aagctttcgc cgcgtactcg ccgggggtgg tgctgatgga gcgcgcgctg
     4081 gagcgcgcgt tcgccgacgc ggcgacggcg gaggtcaatc tagtgaccga ccgggcgtgg
     4141 cacgacgcct ggcatccgga aaaacggcct tacatccagg tgcggatgta cccgcggcgg
     4201 gtgcggtcgc tggtcgcggg agcgtggccg caccgggtgc gcctcgcgct gcggcgggtg
     4261 cgaccgctgc gccgggcggt cgggtggtgg cggtcgcggc ggacggggcc cggagcggac
     4321 tagcgggagc gggggtgggg aaggcgcgcc tgaaagccga taaccgaaac aaggcggggc
     4381 gccggcgacg atcgaccggc gaccgtaaaa tagatatagt acgtccaatt tgtcgtttct
     4441 cgggagcacc gggccggtta tattcgaacc actatgacga acgaagtatt ggtgtcgcgg
     4501 cggccggcgg cgccgcacct ggaagtgcgg gtttaccgcg acccggcgga actggcggcg
     4561 atcggggagg cctgggatga gctcgcgctc ggcgcggcgg ggcggctgcc catgctgtcc
     4621 tatgcgtggg tgtcggccca tctggaactg cgccagccgg cggcgcgccc ctggctgtgc
     4681 ctggcggcct ttgccggcca acagctcgcc ggcgtcctcc ctctggagga gcggcggctc
     4741 tcggttctgg ggaagacggt gacggcgctc cggccgccgg gagatgcgca cacgcgctcg
     4801 gtggatgcgg tggtggcgcc gggacgggag gccgaggcgg ccgaggcgtt ctaccgcgca
     4861 ctcgaggaag cagtccccgg gtggttcggc ctggagatgc cgggggtcga ggggacgtcg
     4921 cccctgattg cggcggcggc ggcgggcgcg cccgctcggg cgcgggcggc gcagatcccg
     4981 gccgggctgg cgacggtgcg ggagacgcag ggtacctggg acgagttctt cgagtcgtcc
     5041 tcggggcact tccgcaagca ctatcggcag tacggacgga agctggcgaa gctggggacg
     5101 gtcgaggcgg agtttttgag cggtgcgcag gcgcacccgg agtacctcga gcgctttgcg
     5161 cggctggaag cggcgggctg gaaggggcgg gcgggttccg cgatcggggc ggacccggcg
     5221 ctgaccgagt tctaccggcg cctctgcgca cggctgcacg cccgccgggc tctcgaatgg
     5281 cacttccttc gggtcggcga cacctatgtc gccgggcacc tggcggtgcg gaccgggcgg
     5341 acgctcgtgc tgtggaaaat cggctacgat gaagcgtacg ctgcgtgctc gccgggattt
     5401 ctgctgatcg gcgagatgct ccagcgcgcg tttgcggatc cggcgacgga cgcggtgaat
     5461 ttcgtgacgg accgcacctg gcttgacagc tggcggcccg agaagcatcg ttacgtggat
     5521 ctgtggcttt atccgcggcg gctgcgctcg ctggtcggcg ggtggtggcc ccggcaggcg
     5581 cggcagcggc ttcgggaaac acccggagtg aaaccgcttc tgcgggcgct gcggggaaag
     5641 ccggagcccc aatgaagtcg ggcgtaccgg ccccgggccg gtgagagggc agaggccgcc
     5701 ccgacgaagg cggagaaggg agcaggtatg cggcaaccgg tgtcggtgat catccccacg
     5761 tacaatcggg cggccatgct cccgcgcgcg ctggagagcg tcctcgacca gctgactgcg
     5821 caggacgaag tggtggtcgt ggacgacggc tcggtcgacc gcacggcggc ggtcatcgag
     5881 gagatccggg ccaaacgggg gagcaccaac ggaacgatcc gctatctctt ccaggacaac
     5941 gcgggagcgg gagcggcccg aaaccgcggc attcgcgagg cggcgcacga cctcgtggcc
     6001 ttcgtcgatt ccgacgacga atggcttccg ggcaaggcct cgatgcagtg cgcgctgatg
     6061 gcggcgcggc cggagctggt tgtctgtttc ggcgatttca agctgcgcta tgccgacgga
     6121 cgggtgatcg accgggggat ggtccggtgg caccaggatg cgcggacgtg ggaggagcgg
     6181 ctcggggcgc ccatgaacct gtccgagctc gcggagtggc cgaaggagca ggacgtacgg
     6241 gtgtacatgg gggacctgta cgcggcccac atgcgggcgt gctacctttc ggcgttcacg
     6301 atgatggtgc gcagaagccg cgcgggagcg ggcttctatt tcaccgaggg gatcaggatg
     6361 tacgaggact ggggatgttt cgggcgcctg acagaggcgg ggccggcggg atttctcgac
     6421 tgcgacctgg cgatccaaaa cgggcaccgg gaagggcgat tgaccgatgc cgatgcgttc
     6481 gtgcgggcgg tgagccgggc ggtggtgctg gaggaagtct ggggggccga cccggagttc
     6541 ctgcgcaccc acggcgagga gtaccgcgcg cttttggacc agcagcgggt ggtgaaggcg
     6601 gcgggatacc tctcgcaggg acaaccggcg gcggcgcggg cgcaactggc gcaggtacga
     6661 aaggcaccgc tgtcctaccg gctcctggcc atgctcccgg caccggccgt gcggggcctg
     6721 ctcgcgctgc ggcgggcgat caggcggggc tgaggcccgc ggcggcggcg ttttatgttg
     6781 ccgcggggag cggcagcggc taccatggca ccgccggccg gggcgccaag gcgccggccg
     6841 cgaaacggat gatctcaaga atgaccactg cgacccaggt gcgatcatat gtcgggcgga
     6901 agccgatcct tggaatcggc ctgctgttcg tgctcgtctt tctcccggtg ctgatcgatc
     6961 tggtgacgcg ctggtgggag gattccaatt actcgcacgg ctttctcgtg ccgttgatcg
     7021 ccggctgggt ggtgtggcgc aagcgggagg aaatcggggg actgcccgcc gaggggagcc
     7081 gggccgggct ggcggttctg gcggcgggga tgctcctgtt cgtgttggga aacgcgggcg
     7141 tcgagtactt cgtggtcgcg gtggccatgg tgacggtgct gttcgggctg accctctacc
     7201 tgtttggggc cgaggtgatc cggcggacct ggtttgcgtt tctgatcctg ctctttatga
     7261 ttccgattcc gggagtgatc tactactcgc tcacgttccc gatgcagctt ctggcgagca
     7321 aggtgtcggt ggcggcgatg cacgccctgg ggatgacggt ggtgcggcag ggaaatgtca
     7381 ttctgctgcc gggacaggcg ctcgaggtgg cggaggcgtg ctcggggatg cggtcgctga
     7441 tttcgctgat ggcgatgggg gcgctctacg gctacctgtc gcagcggcgg ttctgggcgc
     7501 aggtgctgct ctttgcgctg accgtgccga tcgcggtggt cagcaatgtc gtgcgggtgt
     7561 tcgtcaccgc ggcggtcgcc tacgcggggg ggatcgatcc gacggcggag ccgctgcaca
     7621 cgctgttcgg gctgtcggtg ttcgtggtgg cgttcgtgct gctcacgatt gccgcggtcg
     7681 tgctgcggag gtggcagcgg tgaccaaccg ggtgatcatc gcggcgatcc tgctggcggc
     7741 gggcggcgcg ctcggggatt tcttccggtt cgtcgggcgg actcccggcc gaccggccga
     7801 cctcgagggg atcccggcgg cggcggcgga ctaccagggg cgggaggagc cgttcgatgc
     7861 ggccacctac gaggtgctca acgcgacctc ggcgacgctc cggcaatacg cgggcgccga
     7921 cggcagcatg gcggagttgt ttgtcgcgta ctttgcctcc cagcggttcg gcgcggggat
     7981 ccattcaccg cggcactgtt tgccgggcgg gggctggcag atcgtccgcc acgaggcggc
     8041 ggcgctggag gcgccgggcg ggccggcccg gacggtgaac cggctcctga tcgagttcga
     8101 ggggcgccag agcctgatgc tctattggta ccagacgcgc tcgggggtgg tgcgcgggga
     8161 gtacggtctg aagctggatc tgctgcgcaa cgcgctggcg atgcgcccga cggacgcggc
     8221 gctcgtgcga gtcacggtgc cggtggcggg ggacgagggg gaggcgatgg cgcgggcggt
     8281 gcagttcgcc cgaaccatcg acccgtacgt gcagcaggcg ctgcccttct aagagcgcgc
     8341 ggggaccccg gtcatttcaa aagcagcact ttgccggcgg cggtgtgccg ggcatcggtc
     8401 aggcggtaga gaaagacgcc ggagccgacg ctcggatcgg gctcccaccg gacgatgccc
     8461 tcggagccct ccggccggat cacctgccgg tcgacgcggc gcccgaggat gtcgtagacg
     8521 tcgaattgaa gcgcaccgcc gccggcgacg gcgttgtgga aggcgatgct cacctcggcg
     8581 ttgaaggggt tggggaaggc gatcgtctgc agcgcattcg cgggaaggag atagccgggg
     8641 tcatcgtcga cgccgagcga aaggaggatc tcaacggtgt cgccggagga gactgcaaat
     8701 acgccatcgc tgtcgccgca accggccgcg tcgtgcagcg gcagggggaa gcggccgatg
     8761 tcggagctga cgattcggta ggtgagctcg gcccggtcgc ccgggctgag ggtgcgggtg
     8821 acggcgcggt cgggggagtc gatgatccag cggtacgcgg cgtagccggg gattacctcg
     8881 ccggccagcg ggccctcgcg gaggacggcg accgggatgc cgttgacggt ggccgactcg
     8941 ccgacgacgg tgaagtccgg cgggaggttc tcggagaaat agagcccggc gaggggagac
     9001 gacccgccgt tgacgagagt ccgggtgacg gcgagggtgt cgccgagggc cagcacggtg
     9061 tcactgagag tgtaagtgct cgcgacgccg gctccggccg ggtagacggg ttcctgggcg
     9121 gcggccggcg cggccgcaag aaggcccagg accacaaaca ggggtttgct catgctctgt
     9181 ctatcggccg cggcgggggg agatttgaat aaaaagtgcc ggcggcgggg tcaggcgccg
     9241 gaagtcgggg cggacgcctg cttgcgcgag atataggcct cgaggttgtt gagcgagtcg
     9301 aggttatccg ggacaagttc ggtgtcgtcc acgtggattc cgtagcgttc ttcgaggaag
     9361 gcgaccagtt ccaggacgcc ggtcgagtcg atgatgccca tttcgagcag tgagccggag
     9421 tcggtcagtt cttccgggtc gcgcccgatc atgaagttgt cgcgaataaa ttgccgcagt
     9481 tcggtgcgaa cgctcacgta tcctcctgtc aaccaacagc tgctttgtca ctttgccggc
     9541 aacggcccga gggccgggcg gctgcccgtc acccgcatga gacgtcccgg ccggcaatat
     9601 cggcggcgcg ggggccggtg tcaaccgcaa agggggattg cgccccgctg gaccggctgt
     9661 cgctaatatc ggaagattcg ccggaaggtt gaaccggtgc ccgggcggga caggcgccta
     9721 tataatgagg aaaccatcag ccgccaaaat tccgctcgac tccgcgcttg ataaacgcgg
     9781 gcggggcgtg taccttgtcg ggactgggat agcatgagag agaaaccgac gacactaccg
     9841 gaactgctgc aggcggccct gcgttcgcag gtggaactgg ggctgggcga gatgattatc
     9901 gatccggccc gcgtggagac gctggcggcg gaggcggccc gcggggccgc gaaccagaga
     9961 gaggagtggg tcgaccggat ggacgccgcg gtgcggcgca cctcgccgcc gccggtggac
    10021 ctgttcggag gggcgggcgg gacggggccg aaagcggccc aattcggcac cctcgccgag
    10081 caccgggccg ccatctgcga gtgccggaat tgccccctgg gaagagacgc gcacgaaatt
    10141 cgtctacggc gtggggagtc cgcaggcgga gctgatgttc gtgggggagg cgccggggcg
    10201 ggatgaagat ttgaagggag agccgttcgt gggacgggca ggacagttgc tggacaaaat
    10261 cctggcggcg atgtcgatgg cgcgggcgga ggtctatatc gccaacgtgc tgaaatgccg
    10321 cccgccggac aaccgcgacc cgctgccgga ggaaatggcc gagtgcctgc cctacctgaa
    10381 agagcagatc cgaatcatcc ggccgaaagt cctctgcgcc ctgggccgga tcgccgggca
    10441 ggcgctcctg gaaaccaaga cgccgctcgg gcaactgcgg aaacggtggc acgagtacga
    10501 gggcgtcccc ctgctggtga cctatcaccc ggccgcgctg ctgcgtttcc ccgagtacaa
    10561 gaaggaaacc tgggaggaca tgcagatggt gctggcgaaa ctggacgagc tgcggagtcg
    10621 ggcggggtag ccgcgcccgg cgaggaagag cgcgcatggc gtatcgttcg caggaagaga
    10681 aacgggagga gaagctccag ccgccgcagt cgctggatgc ggagcaggcg gtgctgggag
    10741 cgattctcaa agacagcgat gcgatgaacc aggtggtgga ggtgctcgac gacgagagcc
    10801 atttctacgc ggccaggcac caggcgattt tccgcgcggc caaggcgctc tacgagcggg
    10861 gggaacccac cgacatcacc atggtggcca acgagctgct gcgcaacgac gagctggggc
    10921 ggatcggcgg gcgggtgtac ctcgtggaac tcgtcgaggg agtggcctcg acggccaacc
    10981 tctcggccca cgcgacggtg gtgctcgagc ggtcgattct ccggcggctg atcacgacct
    11041 ccaacgaaat tatcaagagc tgctacgcgt tcgaacagcc ggtggccggc ctgctcgatc
    11101 gggcggagtc gaccattttc cgtctgtcgg agagccggct gcggaagggc ttcacctcga
    11161 tccgggacct gatcccctcg acgtttgaag acatcgaagc gctccagtcc aacgagagcg
    11221 gactgaccgg gctcccgacc gggtacgagg aactcgacct gatgaccaac gggatgcaca
    11281 agggggacct ggtgattgtg gcgggacggc cgtcgatggg gaaatcggcg ctggcgatga
    11341 acatcgcgga gcacgtggcc atcaagcagc gcaagggcgt gggggtgttc tcgattgaaa
    11401 tgtcgaaaga gcaactggcg ctccggatgc tgtgcgggcg ggccgggatc tcgcagcaga
    11461 aactgcgggc gggcaagctg cgggacaccg agtggagccg gctggccaca gtgggcggga
    11521 tcctgtcgga ggcgccggtg tttatcgacg attccccgac gatcacctcg ctggagatcc
    11581 gggcgaaggc gcgacggctg aaagcgcagc aggatgtcgg cgtgatcatc gtcgactacc
    11641 tccagatggt ccacgggtcg gggcggttcg aaaaccggca gcaggagatg gcgaccatct
    11701 cgcaggggct caaggcgctg gcgaaggaac tggagatccc ggtgatcgcc tgctcgcagc
    11761 tctcgcggct ggtcgaacag cggggcgggg agaagcggcc gcaactgtcc gatctgcggg
    11821 agagcggggc gatcgagcag gatgccgacc tcgtcctctt catttaccgg ccggaacact
    11881 acctcaccca cctggagcag aacgatccca agatgcagga ggtggcgggg ctggcggaga
    11941 ttatcgtggc caagcagcgc aacggtccga cgggggtggt gaagctgcat tttcggaagg
    12001 agtttgcccg gtttgagaat ctcgaacggc accaccgcga gattccgccg ggagtggaac
    12061 cagtggccgg cggcgatgcg ccattctagc ggagcggtat gaacctgggc gtaacgacac
    12121 tggggcggca gggggtcaac gcgatcgacg aggtcggggg gatggcgatc ctgttcggcc
    12181 ggtttctcgt cgccctctcc gggttgcccc ggtcgtttcg gctctatgtc gagcaggtgt
    12241 attcgctggg ggtgcggtcg ctcccgctca tcgtgatcat ctcggtcttt gtcggggcgg
    12301 tgtcggcctg gcagggggcc taccagtttt cgttcatcgg ggcgccgctg cgctttctgg
    12361 gccaggcggt cggcaaggcg gtggtgatcg agctggcgcc ggtgctctcg gcgatcgtgt
    12421 tcgcggggcg ggtcggcgcc ggggtggcgg ccgagctggg gacgatgcgg gtcaccgagc
    12481 agatcgacgc gctcgagtcg atggggatcg acccggtgcg ctttctcgtc atgccgcgca
    12541 tcctcgcctg cctgacgatg gtgccgttcc tggtcgtgtt cgccaatttc atcgccattc
    12601 tcggcggcat gggggtggcg gtgcttgggg tggacatttc ggcggaaatg tttctcgacg
    12661 gctttcacag ctcgttcagc ctctcggatt tcatcaacgg ggagatcaag gcggccgtgt
    12721 tcggcctgct catcgggctg gtcgggtgct acgaggggtt caacaccaaa ggcggggcgg
    12781 ccggcgtggg ggaggcgacc accacctcgg tcgtcatctc gtcggtgctg atcctggtct
    12841 cgaatttcct tttcgccatc gtgctcttcc ggttttgacc tatggataca ctacggcccc
    12901 aggcagagag cgtctgtttc gaggtggagg atctccacaa ggcgttcggc gcgcagcggg
    12961 tgctcgacgg cgtcagcctg aaagtgcaaa ccggggagtc ggtggcgatc atcgggcagt
    13021 cgggctcggg gaaatcggtg ctgctgaaac acctgatccg gctgctggtg ccggaccgcg
    13081 gccgcgtcct ctaccggggg cgggacctcg ccgacatgag ttccggcgag ctggtcgaag
    13141 tgcggcggaa aatcggtatg ctctttcagt cggcggcgct gttcgattcg atgacggtgg
    13201 cggagaatgt cgggctggga ctgcgggagg cgcggcggca cgagcagaga gagatcgacc
    13261 ggattgtcgc ggagaagctc gagatggtcg ggctggccga ggcgggggac aaatatcccg
    13321 ccgagctgtc gggcgggatg cgcaagcggg tggggctggc ccgggcgatc gccaacgacc
    13381 cggacgtgct gctgtacgac gaaccgacga ccgggctgga cccggtgacg gccgacgtaa
    13441 tcaatgactt gatcgtcaac ctcaaccggc agctgcgggt gacctcggtg gtggtgacgc
    13501 acgacatgaa gtcggcgttc cggatcgccg gccgggtggt gatgctctat gacgcgcggg
    13561 tgcggttcga cggcagcccg caggaactgc agcgggcggg cgacccggtg ctccgacaat
    13621 ttatcgccgg cgactccgcc gggccgatac ggatatgacg ggaagtcggg cggcgatggc
    13681 ggacaagcgc agcggcaaag tgaagacggc gtttttttgc acccagtgcg gggccgagca
    13741 cacccgctgg caggggcagt gccgggagtg cgcggagtgg aacacgctgg tcgaagagcg
    13801 gctggtcaaa ccggcggggg gcaaggggcg ggggacgcgc ccgccggccg aggcgcggcg
    13861 acttccggaa atatcgctcg ccgcggaggc ggggatcgaa agcgggatcg gcgaattcga
    13921 ccgcgtgctg ggcggacggc tgctgccggg gatgagcgtg ttgatcggcg gcgagccggg
    13981 gatcgggaag tcgacgcttc tcttgcaggc ggccgaggcc tactcgcggc gggggcaccg
    14041 ggtcgcttac gtcacgggcg aggaatcgct cgcccagatc cgcctgcggg ccgagcggct
    14101 cggcgtggcc ggcgaaaaca tcgtggctgt caacgccaac gcgctggaag aaatctgcga
    14161 cctggtggcc gagggcgctt acgagatcgt gctcgtcgac tcgatccaga cggtggcctc
    14221 ggcgatgttt gactcgcccc cggggacggt cgcgcaggtg cgggagtcgg cccagcggct
    14281 gatgggactg gcgcggcagc acgggttcgc cctgttcctc gtggggcacg tgacgaagga
    14341 tgggatggtc gcggggccga aggtcctcga gcacatggtc gatacggtga tctctttcga
    14401 gggcgacggg agccacctgt accggatgct gcgggtgatg aagaaccggt tcgggtcggt
    14461 ggccgagatc gggctgttcg agatgcagtc gcgcgggctg gtggaggtga ccaatccctc
    14521 gtcgctgttt ctgtcgcagc gccaggatgc gcgccggacg gggtcggtgg tggcgggcat
    14581 ctgcgagggg cagcggccgc ttttggtcga gatccaggcg ctcgtgacgg ccgccagcta
    14641 cggcaacccg cagcgggtgg ccgggggcat cgataacaaa cggctggccc tcctgatcgc
    14701 gattctcgaa aaaaaggggg gctttccgat ggggatgaac gacgtgttcg tgtcgatcgc
    14761 cggggggctg cggctgagcg aaccggcgct cgatctggcg atgctcgcgg cgatcgcctc
    14821 ctcgcttctg aacaaaccgg tcgatccgcg gacgatggtg gtgggagagg tcggtctgtc
    14881 gggggaggtg cgcggcgtga ccatgatcga ccggcggctg gccgaggcgg ccaagctggg
    14941 gttcacggcc gcggtgatcc cggcgatgaa cgcggctcag gccaagcggg aactgccttc
    15001 ggtgcacggc gtggggtcgc tccaggaggc gatcgaggtg ctgctcgggt gatacggatc
    15061 ctttgccgcg acggcgcggc gcggcggggg gaagcggcga ccgcgcacgg cctgtttcag
    15121 acgcccgcgt tcatgccggt cggcaccgcg ggggcggtca aagcgctcac ctgcgaggac
    15181 ctcgaggaga tcggtgtcga aatcatcctc ggcaacacct accatctcta ccttcgcccc
    15241 ggccccgaga ccatccggga gagcggcggg ctggcggcct tcagcggttg ggggaagccc
    15301 acgctcaccg acagcggggg gtaccaggtg ttctcgctca aagaccggct gcggatctcc
    15361 gacgacggcg tggagttccg ctcgcaccac gacgggtcga cgcacctgtt cacgccggag
    15421 aaggtgatac agattcagca cgcgatcggc gcggacatca tcatggcgtt cgaccagtgc
    15481 gtgccctacc cggcggagcg gcgggcggtt gaagaggggg tgcgccggac gggggagtgg
    15541 gcggcgcgct gtgcgcggga gcacgagcgg cgcagcgaga gtggtccgga ccccggccgc
    15601 ctgttcggga ttgtccaggg agggacctac gccgactttc gcacgcgctc ggctgagcag
    15661 atcctcgcgc tcgatctgcc ggggaatgcg atcggcgggc tatcggtggg ggagagcaag
    15721 gccgagatgg aggccatgct ggcgcacacg gtgccgctgc tgccggcgga caagccgcgc
    15781 tacctcatgg gggtggggta tccggaggac atcctgactg cggtgtcgta cggggtggac
    15841 atgttcgact gcgtgctgcc gacgcgcaac gcgcgaacgg ggaatgtgtt cacgacggtc
    15901 ggaccgatca cctaccgcaa cgccgcgtac gcgcaagacc agcggccgct cgacccgcac
    15961 tgcgggtgcc gcgtgtgccg ccgctacacc cgggcatacc tccggcatct ctacaaccag
    16021 tcggagatca cggggatagt gctcgcgagc taccattccg tgtatttctt ccaggaactg
    16081 atgcggggga tccggcgggc gattgaggaa aggcggtttg acgcgtttcg gcgggaattc
    16141 ctggcgaggt atgcgggaga gtgaccgggc ggcagccgcc gccggagtcc cggcgtccct
    16201 gtcgcgcctg gccgggaggg ggtaatccgg aaccctgtga ggccggtggg ggcagcagtt
    16261 ccggcgccat cctccgacga tccgttgggc gccggtcggg gagtggagcc atcacgcgtg
    16321 gtcgggcgcc cggcggccct tccgggctgt cagggtcggg ggtccggcgg cacgggtttc
    16381 ccgcacaccc ggcagtccgg ccttttctcg aaccccacca tctcaaaggc catcgtatcg
    16441 gcgcggaaaa tcagaagcgt gttcgaaacc ggcatgtcgc cggtcatcag gtagcgcagc
    16501 gccagcgttg cctgaagcga tccgatcacc cccggcaatg ccccgaacac cggcgtcggc
    16561 ccggcgtgca gcgcatcctt gtgcaggaga caggccaagc aggcggtctt cgggggattg
    16621 aggaaactca gttgcccgag ccattcatgg atagccccgt agacataggg aatgctgagg
    16681 cggaaagaga catcgttcag tatcaaacgc ccggcgtgat tatccaggcc atcgatgatc
    16741 acgtcgcatc ccgaggccag ctcgccggcg ttgtcctcgg tcagcttcag cgggatggct
    16801 tcgacggtta tttcgggatt ctgcgccgcg agcttctcgg ccgcaagaac cgccttgggt
    16861 ttgccgatgt ccccggcggc aaagagaaac tggcgattca ggttggagag ctcgaccctg
    16921 tcgccgtcgc atatgcacag gtgccccacg ccggctgcgg ccaggcaggt cgcactaatc
    16981 ccgcccagtc ccccggcccc gacaaccagg acgcgcgcgc ttttgagtct gtcctggttc
    17041 caacggtcaa ccgggagttg gcgtcggtac ctctccattt caggtttcga caggggcatg
    17101 agaaggggtt ctcagccgcc ggagaccggc ggctggagag tgattgtctg gccgttggat
    17161 ggcttgaagg acaaatcctt cacgtggccg tccacgtaga tgacggcaac ataggcgggg
    17221 tcgagcgaaa tagcctcgag gatttctccg atcgtcctgt cgccgtcaat ctggaccctg
    17281 cgggtatagt gatcgacatc aggtctcagc atcgcgcgca gctgacctcc ggctcgaagg
    17341 aagactatca tgtttaattt cctcaaagac gggcaacggc gcggacgcgt caccttggag
    17401 gcacggcgag cccgccgcgc cgaaacggtc cggcagtcgg cctcgttacg ctgtccgcgc
    17461 cccgacccgc tccgctgaga gagacgaggc ccgccgcggc ccagggctag aacgctttcg
    17521 gtttatatcg ttctccgcga actgccggga tgccattcgg gagcggcttt cccaagagcc
    17581 cgatcttttc gatattgtcg gccgcgaaat ggagtttcag gtcatgcagc acttcgcggc
    17641 tcgggttgcc atcggcatcc cacccgcgcg cggcgtaata ctcgtcgagc attgcctcga
    17701 tctggtctct ggtcagcccc ttgccggcac tggggccggt cgggatcttg tcctcccagg
    17761 atcgcgccgg cgggtagtcg aaggtgcggc cgttggcccg gttgcgttcc aggtaatggc
    17821 atcgcacgag attccagatt ttctcagaag ttttgagcgc ttcggcagta tcgacattcc
    17881 acccggtgat ataattgagc gccacgacaa tctcctccgg ctcgacgtcg agctcgcccc
    17941 agaagaggcg gcagatgcac cagcagtcga agagcggccg gatgtgctgc aggtacacaa
    18001 cgaccttagc acgtccctcg atccggtcgc gtccttcggc catgtcgacc gtgatcgtcc
    18061 acgagcggtt gtgatgggcg ccgatgtcgc tcgtcatata cgagagcagc atgcccgggg
    18121 cgtagcgtcc gtcatacccc gacatttcct ggcccttgac ctgcatggcg aatttctcgc
    18181 tgccccgccc gattttctcg gcggcccgcc tggtgccgtc cgcgaaatag tcgccgatcc
    18241 ctcggcggtg cacaatcatg tcgatgaagt gttccgcgtc atcgacagaa ccccagttga
    18301 gcgtgtgccc ttcgaggtcg tcggcgctga tcagcccgcg ctggaagcac tccataccga
    18361 acgccaccac gccgccgccg gacatggtgt ccatgccgac gttgtccatc agccagttca
    18421 ggtaggcgac ctggttgatg tcggtcatgc cgaggttgga gccgcacagg gcgccggttt
    18481 catattccgg gccttcgaga tagaccgttt ccttgcccgg gacgaccgcc ttggtgtact
    18541 tgccgcagtt catccagcag ccgaagcagg ctttgtgcgt gatgaggcac ttttcgacca
    18601 gggcgtcgcc gtcaatgccc ttggaattct catagtaggt ggtctggaaa ttccgggtgg
    18661 ggagaaccgc gttgacgttt gaccagtcga taaagaatgc agtgccgtat ttctgccacg
    18721 gcgccatgtt cggatgggtc tgcgtccgct tgatgatggc cgacacatgt tcggtgagcc
    18781 ggtcgagatc gtgcactttg atcccgcggg taccgcggac agcgatggct ttgagctttt
    18841 tcgagcccat gaccgccccc tgcccggtcc gccccgcctg gcggccgaaa tcgtgggtga
    18901 tgcaggagaa gcgaatgccg ttttcgccgg ccggcccgat ggtggcgatt tcgaaatcct
    18961 cgcccaggtc cttcttcatg gccggttcgc actccaacga gcccatgccc cagtacgggg
    19021 aggcatcgcg cagttcgacc gtgtcgtcat cgataaagag gtaaaccggt acgggggaga
    19081 tgccggacat gatgaccata tcgtatccgg cgaacttgag ggccgggccg agatggccgc
    19141 ccatgttgga atcgccatgg ccgttggtca ggggggaaat ggagccgaat tcgactttcg
    19201 cgcccgccgg gataaagcac ccgctcataa tcccggcgtt catgatgaac aggttctccg
    19261 gcccgaacgg gtcggcatcg cgggggacct cgtcgtagag aatcctggcg atcgtgcccc
    19321 gtcctccgaa ccattcgcgc accaacttcg catcggtggg gtgcctcctg atctccccgg
    19381 tagtgagatt gatgcgcagg gtcgtgccgg ccatgcctcc acagctcgcg gactcgtgcg
    19441 ccatcactta ccctcctttg ccggcctggt gaagacggtg atggcgccgg tgttgcacag
    19501 ctcggcgcat tggaagcagt tgtcgcagat gatgacgtga tcgacatcgc gatgctgaaa
    19561 tatcacacct ggcttgcaga ccgcgatgca gtcgccgcaa ttggtgcact tttcggcgat
    19621 cacgatatag gcgccgtcat cgcgacggaa gatggcgtgt tcggggcagg cctcgaggca
    19681 tcggccgcac tgcgtgcaga ctttgggctt gtacagcccc ggctgcggaa agagaacctc
    19741 gattctcaag ctggcctttc tcggattgat ctcgtggtag tggctgatgg cgcaaatctg
    19801 catgcacagg cggcacccgc tgcacttgcc cggcgaaact ttgagtctct ttgtttccat
    19861 gatgccctgg ttgactccta tgctttcgcg tatacctgcc cggcaatcca gatggcggcc
    19921 acgagcacga cggaaacgac tcggaacagg acggccggcg cgcgccattt gcgcaggagg
    19981 gcgtagggtt gaatggcatt gcccaggtaa taaccgaggt ttatcgtcac aatcgagatc
    20041 agcgaggcgc tgacagcgtc ggaggcaagg aacgaacgaa tccccgccca cccgtttgcg
    20101 gcccacgagg cctcgcgcag gacgtacgcg gcgatgagcg cgaaggcggc gtacgaggca
    20161 tagacaatga ccggcgcggc gagaactctg tagagcgggc tggaatctag ccgttcacgg
    20221 aacgccgagt aggcgatatc gaagacgatc ccgttgatca tcaccgccgc gaactggcag
    20281 gggaagaagt agctggaggc ggttttgaac aggccggcga tcagcgccat gaccactacg
    20341 gagccgggaa agtcgacgag ccgcttggag gcgtagagga tgatgatgcc aagtccaaag
    20401 agcacggccg atttctccgg cgccccagcg gctcggagca ggtcacgccc gaagagctcc
    20461 agtgcgcccc acagcgaacc gagaacgagg aggacgagga tcttttcata tcttctcatg
    20521 gtctgctcca gcaggtggac gctgatgttt ttgttggccg gtccccgccg cggtcgacct
    20581 gcggccgcaa gatctcaggc caacctcctt agcgacaaca tgatctgtca tgcggcacgc
    20641 ctgcccgcag gctgccatct gtatggacct ggaatcctac ggacaagaca ctctatatta
    20701 atacacgcct tcccttatag caatgacttt cggagtgttg tcggcggttt tgggcggtca
    20761 cgattcgtcc gaatgcgttg tgaccgcagc cgcaccccgc gttcttcgag ccgattgttg
    20821 tgtgccggtc cggttaagaa gcgacctcgg ccctgctcca ttgcggccgg cgtacggtgc
    20881 ctgatttggg gacccctggc cgtccgccgc ctactcgaat acgagctcgc cgaaggcagc
    20941 cgggtcgtag gaccaggaag tctggaacga tgccgaggag ttcatccgca cttgtcggcg
    21001 gcggaagttc agtcgccagc gatctccgga gtcgggcgtc gctgcgaact gcgcgagcgg
    21061 gatacgcatc tcgacggcgt agaagtccgc cccgcggacc gttttcacct cgaaatcgcc
    21121 gttccagtcc gagcggccgg cccagtaccc gtcgctcgcc tgctggactt gctgatcata
    21181 cagggtcccg agggcgttga catacacctg ggcgacgacg cggttggtcc cgcccagctc
    21241 gatcatccag ccgactgcat cctccgtgaa cacgggggcg tcgcgctcgg tcatcgcggc
    21301 atggatcgcg gccatgtccg aatcgcggca atgcgcggcg aggtacaggt tgtcgctgtc
    21361 gtaggcgaag tagaacatgg tcggatcctg tgccgccggt gtcccgtttt tgctgaacag
    21421 cgcggtgacc ggccgcgccc agcagggttc gatcaactgc ccgtcgataa cgactggttc
    21481 attcacccgg atgcaccggg cggcgcgggc gacctccaat tcgcggctca cccttgtcga
    21541 tttttccgcg gcgtagggaa gccgggtgga aatcacaggc agtggaagca gcgcgggcgc
    21601 cggctcaatg gcaaactgcg cggttacggt cgcccccggg accagcgtaa atggacaggc
    21661 cgcgggcgta attgtccaac cttcaggtac gtcccagcgc aaggtgtcgg tccattgtcc
    21721 ccctgccgtc ggaccatcca gcagcacggt cactgccccg gctgccggct tcagcccgtc
    21781 ggtgagcggg agcggctgtg cgaacctgat cccgatgctc ctgacccgac tggcggcccg
    21841 cgcctctgcg acggtctgca catcccacgg cagcaccgca tccttcttga tgggcgcgat
    21901 gtgtacgcct cgatcatcga ccgttaccca gccgaagtga taccgcagac catcggggct
    21961 ctcctcggtc tcggcgccgg aactgccgag ggatgtgtac aggatgccgt caaactcggc
    22021 cgagaagtag cagtgcatat gaccggtgaa aacggcatcg accccgttgg cccggaagat
    22081 gtcgtgcagg gcgtccggct gcccgctgcc cagtgtccga taccagaacg gcttgtggag
    22141 aaagacaatc gtgtagcagg cactgcgatt cttcgccaga tcgtctttca gccagccgag
    22201 ttgctcctgg gcgatctcgc cggcgctctc tgtacggctg ttgtcgagca ccacgaaatg
    22261 gatgccgcgg tggtcgaagg cgtagtacgg ctcccctgcg cgggcgcgat aggttgactc
    22321 catggcgtct gtgctgatat cgtcgttgcc gggtgtgaag cggaccggca tggtgagcgg
    22381 tctgataatg gcgaagtact catcccaccg gctgcgcatt tcgatcgtat cggtggtgtg
    22441 cccctcgatc atgtcgccga cggtcaccac gaagtcgggc cgcagccgct caatctcggc
    22501 gaccaccgat tcgtacactc cgtcctggtg gtcaccggta cggtcgccaa gaaccgcgaa
    22561 tcgaaccggg gcgtccgata gatcatcgca tggcgtctgg gctggagctg gagatccgag
    22621 ggcgatgagg acaaggccaa atatggcggt agttttcatc acgtcgcaac acccgtcttt
    22681 gagtttgccg gttttgcccc gaaagataca ccgggtccgc gtccgacaca atgcaatctg
    22741 aagtgttcgg tacctgggcg agggacgaga atccgccaaa ggggtggggg aaccggcaga
    22801 gcgggcggcc gccctgccgc ggctcgcggc agtacggtcg gagcggtcaa ggagcgcggg
    22861 cctttcggat gctccgggcg acggcatggg cggctagagc gggacaaggt agccgacaga
    22921 catgcggaca tcgtggaggc ggacgggttg ggagaaagtg cggaggcggc tgtcgccggt
    22981 gtcgccggtg atgcggatgg tggggcgcgc ctccgacatg ccgatttcga gcgagaagtt
    23041 gcgattgaag cgaaggccga gggtcgcccg atagccgatt ttcggggcca tggcgatgtc
    23101 gaggccgacg gcgccgggaa cgaaatcggt cggttcgagg ccgtcgaaac ggaagtatcc
    23161 cttgtagagg agggggccca tgccgacaaa cccggtcatg gcatcggtgc cccggtagag
    23221 gaggatctcg gcgctgagaa tcatagagtg cagcgcaccg tcggggtagg tctttgagcg
    23281 catggcggcg aatccgtaat cggcgcaggc gcgcaggagg aacgggtggc ggaggaggaa
    23341 ttcgtacttg gcggagatga ggagaccggg atcggtgagg acgttgggtc cgccgacacc
    23401 gatcgtgaaa tgcatgttga cgccgttcac cgtgtcctca acgaccgggt tgagcgggta
    23461 gagcggggaa accagccagg cggcaatcag gatggtcagc atactcgtag ggcctcacag
    23521 cggtgatcta aaagaataac gcaccgcgat cagaaaaagt cgaagaaaag ttgcggggat
    23581 ggggccaggc cgcgaagggg cgcgattgcg gcactggcgg ctccggagcg ggggaagccg
    23641 gcgccggcag cctgggtgcg gcaggcggcc gggtgaggtc atgcaagcgc ttgcggcaca
    23701 aggagcatat cgatctgatt tgaccgcgaa agcagttgac aaccgagcga gggagggtat
    23761 attaacgctc tgtgtatgcg ataaggtgtg aacaaattca caaaatgaca cggcgtcgtc
    23821 cgtggtaagt gtctgtaagg aataacctta gaacaaacaa ctatgagtga gccgagaact
    23881 ttcctgaatc tcatcaaggg cgagtgggtg cggtcgaagt cgggcaagac gtacgacaac
    23941 atcaacccgg ccaacacgga tgaagtggtc ggcgtttttc agaaatcgaa cgccgaggat
    24001 gtgaaagacg cgattgatgc cgcggccgag gcctacaagt cctggcgcct cgtgccggcc
    24061 cccaagcggg gagatatcct gttcaaagtg gcggcccgcc tgcagaaaga gaaagagaat
    24121 ctggcgcgcg acatgacgcg cgagatgggg aaaatcctcg acgaggcgcg cggagacgtg
    24181 caggaagcga tcgacatggc tttctacatg gccggggaag ggcggcggct gttcggtcag
    24241 acgaccccct cggagttgcg caacaagttc aacatgtcga tccgccagcc gctgggcgtg
    24301 tgcgcgttca tcacgccgtg gaatttcccg atggcgattc cctcgtggaa gattatgccg
    24361 gccctggtgg ccgggaacac ggtggtgatc aagccggcga cggacacgcc gctgtcggtg
    24421 gttaacctgg tgcggatcat gcacgaggaa ggggtgccgg ccggcgtggt caacgtcgta
    24481 accggctcgg gcggagcgct gggcgaaccg ctcatccgcg acccccgcgt gcgcgtggtg
    24541 tcgttcaccg ggtcgaccga agtggggagg aagattgccg agatgtgcgc cccgcagttc
    24601 aagcacacgt gtctggagat gggcggcaag aatgtccaga tcgtgatgga tgacgccaag
    24661 ctggagctgg cggtggaagg ggcgctgtgg ggggcgttcg ggacgaccgg gcaacgctgc
    24721 acg
//
LOCUS       DAOPOE010000047        23597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2708_length_23597_cov_5.555985, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000047 DAOPOE010000000
VERSION     DAOPOE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23597
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_23597_cov_5.555985"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKM94_07730"
     CDS             complement(<1..406)
                     /locus_tag="PKM94_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005275015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOD66571.1"
                     /translation="MTRKIRKILVANRGEIAVRVIRACHDLGIGSVAVYSDCDHAALH
                     VRMADEAYHIGPSPAAESYLVHENIIAAARRAGADAIHPGYGFLAENADFARKVVAAG
                     LVFIGPAPETIALQGDKLAAREVAQRAGLPVTP"
     gene            complement(419..1969)
                     /locus_tag="PKM94_07735"
     CDS             complement(419..1969)
                     /locus_tag="PKM94_07735"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOD66572.1"
                     /translation="MSIEDRLQRLEAARREARLGGGQERIDAQHAKGKLTARERIELL
                     CDPNSFEEFDMFVTHRSSDFGLDKQRILGDGVITGCGKVHGRLVYIFSQDFTVFGGSL
                     SEAHAEKICKVMDMAMKVGAPVIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVVP
                     QISAILGPCAGGAVYSPAITDFTLMTKGTSYMFVTGPNVVKTVTHEEVTSEDLGGAAV
                     HASRSGVAHFACDNEADTIAKIKRLLEYIPQNNCEDPPVAECADPPGREDDLLNTLVP
                     DNPNQPYDMKEVIARIVDQGSFFEVHEDFAPNIIVGFAHLGGRSIGIVANQPAVLAGV
                     LDINSSRKGARFIRFCDCFNIPILTFEDVPGFLPGTDQEHNGIIVNGAKLLYAYCEAT
                     VPKVTVITRKAYGGAYDVMNSKHVRGDMNYAWPTAEIAVMGPKGAVEIIFRKQIADAS
                     SPELETQRQTEMYREKFANPYIAASRGYIDDVIEPKTTRPRLIRAFEMLATKKDSNPP
                     KKHGNIPL"
     gene            complement(2040..2924)
                     /locus_tag="PKM94_07740"
     CDS             complement(2040..2924)
                     /locus_tag="PKM94_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOD66573.1"
                     /translation="MPTVFLCHSSKDKPFVRGLAAELTKQAIDVWIDEKEINVGDYLA
                     ERVSKGISDSKFMVVVLSRNSVDSRWVQVEMNKGMSKEIEDRRTVVLPIVIDDCQIPP
                     VLQGKVYADFRSPSDFEEPFRLLLRAIGISELPEAISNPQASEATTGLASQVQVVKHP
                     EDVLHYFPDITVLGVDRGNVKRIDDVGGGLALYDVPFILSVTPPAGWKSIFDEERAHP
                     RHTMWRKARVEEDRIVVRCALYEVRQYHFDDIRQDVAASNQKYRDSLVKQEREYRDRV
                     RQESDEQERIDDALEGLD"
     gene            complement(2937..3791)
                     /locus_tag="PKM94_07745"
     CDS             complement(2937..3791)
                     /locus_tag="PKM94_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD66574.1"
                     /translation="MRLGDCDITPFVERRFRLDGGSMFGVVPKAIWQTLLPADGENRI
                     AMVTNLFVLRGRGAVTLFDAGFGDTLTPREEKIYGVESASGLEEGLAGLGLAPADVTR
                     VVLSHLHTDHAAGAVRRADGRFVPRFPGAVYVAHRREWEAAMHPNERTAAVYSPERLQ
                     ALADAGQVQFIEGDTVLSEGLRAVPTGGHTEGHFGLEIEGGGRKVWYYADLFPTAAHL
                     KPAYVPAADLYPLETMAVKLRLLPRIVAEGVILAVDHDTAMPLATLAMEGTRTVARSV
                     EQYRQRHR"
     gene            complement(3791..4936)
                     /locus_tag="PKM94_07750"
     CDS             complement(3791..4936)
                     /locus_tag="PKM94_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD66575.1"
                     /translation="MNRNIDPRQQAVRDQVKQFAEKEIYPITRELDQMPEPRRFPTEL
                     YRKIGAAGFIGYAMPKELGGSGKSHLEYITLVEELCYHDPAVGLLCAVGELATHPIIH
                     FASDQQKKKYVPDCARAARIPAFVLTEPNAGSDAANQQTEAVEGDGGFILNGEKIFIM
                     HGDAADLAVVFCKVKDKPKLSAFIIDDLKQPGWQARTLRQKMGMRAATTGGIHLKNVF
                     VPKENLLGEFGKGFRYAMTTLDSARVGVAAQGVGIAQRMLDEAVAYAKKREAFGAPIA
                     KLQAIQWMIADMATRLAAARHLTYHAAKLQDAGESFGLEASMAKLYCSETARFCVDRA
                     MQIHGGYGYIGEFSHIEKFYRDQRVLEIYEGTSEVQRLVIAGNVIGR"
     gene            complement(5044..5463)
                     /locus_tag="PKM94_07755"
     CDS             complement(5044..5463)
                     /locus_tag="PKM94_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOD66576.1"
                     /translation="MPDKIRVLLAKPGLDGHDRGIKVIAAALRDAGMEVIYTGLRQTP
                     PMIVDAAIQEDVDAIGVSILSGAHMTLFPAILEEMKKKGAGDIILFGGGIIPEDDKAA
                     LENMGVGKIFTPGAPTEAAIEYLREAVAKKQKGEQIM"
     gene            complement(5510..7207)
                     /locus_tag="PKM94_07760"
     CDS             complement(5510..7207)
                     /locus_tag="PKM94_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD66577.1"
                     /translation="MFLKKFLDQLKAHRQTWDQTAEKYRAQKSMPRFFTVSGVDINDL
                     YTPDSIAGADREDYFWRNLGFPGEYPYTRGVHHTLYRTKLWTMRQFAGMGTPRQTNER
                     FHYILANGGTGLSTAFDLPTLMGYDSDHPRSLGEVGKCGVAVDTLADMEIIFDRIDLG
                     RVSTSMTINSPASMILAMYLAVAEKQGVPFAAVTGTLQNDILKEYIAQKEFIFPPRPS
                     LRLITDMIAYCTRHVSKFNTISISGYHIREAGSTAVQELAFTLADGFQYVDSCLEAGM
                     GVDEFAPRLSFFFNAHSDFFEEIAKYRAARRIWARHLRERYRAQDERSWKLRFHTQTA
                     GVSLTAQQPENNIARVAIQGLSAVLGGTQSLHTNSMDETLALPSEKAARIALRTQQII
                     AYETGVANTIDPLAGSYFVEALTDQMEAGAEAYFREIAERGGVLKCIEDGFFQQEIAR
                     AAYAHQQELERDERVIVGVNRFQLENEEIDIPVLRIDPQIERDQVAFLKKVKAERDRA
                     RVEASLETLRRVARGQGNTFEALLECARAYASVGEMCDVLRQEWGEWVESQAAMMPS"
     gene            7561..8742
                     /locus_tag="PKM94_07765"
     CDS             7561..8742
                     /locus_tag="PKM94_07765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD66578.1"
                     /translation="MLGIDYGDPWDIRTFSGSSRYLWSEFRRQGVLVDAFTPYPSRPL
                     TMFYKARAFHPNMRRWRARWRRSLPFRKCLSARAVRKILRNYRGAFNASLQIGAYYNL
                     AAALDCPRGLLADGNCALSQRTYATYQTSDAIFRKQREFERAVYRSMDRVFCFSRFLA
                     QSMIDDFGCAPERVKVVGAGINIDETLIRNPERNYGAKTILFSAFDWVHKGGPVLLEA
                     FEKVKREAPEARLILLGPTLTGLPQGVICHGPLSKDQPQQLQTLGEAFRSASIFVHPT
                     LADAFPNVIREAMAAGLPCVASDIGSIPEMVTEGQTGYLVPKGNAAQLADRVLRLLAN
                     PGLAQAMGEAGYARYRERFTWARVCATIVDELGQVPAERSTGAWPADQTMETALRDSA
                     E"
     gene            8996..11320
                     /locus_tag="PKM94_07770"
     CDS             8996..11320
                     /locus_tag="PKM94_07770"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOD66579.1"
                     /translation="MRDCISLIIAAILVAAAAAPANCRPSTPTFTENRGQWPDSILFR
                     AEDGPTALWFTTGGVYYQFTRVLGDRHAAGRSALSTDQVARKPEAAELLTVKAALAGA
                     NRAATATGVNPTASRCHFFLGDDPSRWRTEVANFAEIRLTEVYPGIDLVYRGIAADPG
                     SPGGAVEYDFRIAAGADYTQIRVRYEGAEGLAIDPEGGLVIKTAWGDLRESRPVAHQE
                     AHGVRWSVEAAFVITGPREFSFRLGAGYDPSQPAVIDPVLVYGTFLGGTDREQGNGVA
                     VDNAGAIYVAGTTYSADFPTAAPYDGTLGGDADLFVTKLAADGSDLVYSTYLGGSGAD
                     YGFDLAVTGEGSAAVTGQTYSTDFPTVSAWQTDPDVPDGASADAVIAKLAPAGNSLTF
                     STYAGGTNAEYAWGIALGADGAAYVTGYTLSDDLPTINAIQTFQGGADAFVVKIPSAG
                     GPALYSTYLGGEGEDWGRGIAADAAGAAYVTGHTRSTAFPTQSPFQGDQPGHDAFAVK
                     IAAAGGSLVYATYLGGSAYDAGMGIAVDGAGFASITGRTYSNNFPTQNAYQANQDTLD
                     AFVVKLTAGGNGLVYGTYLGGNGLDEGWDIALGSDGGAYITGNTYSTNFPQSYAYQGD
                     QGTADAFVTRLSPNGRSALWSTYLGGQMIDLGTDIARDSGGGVYVAGYTYSDDFPTES
                     AYQAARTGIDLFVAKFDAACCTGIRGNAEGDSNDNITVADITFLIAAVFRGGPLPECF
                     EEGDVSGDGELKVGDITYLIAYVFRGGPAPAACP"
     gene            11564..13966
                     /locus_tag="PKM94_07775"
     CDS             11564..13966
                     /locus_tag="PKM94_07775"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66580.1"
                     /translation="MTFTSRLIARAAAAFLLAGGLLEVGLAQSRPALPPPEPAGSGTS
                     DAAFGHPDDVFWDNTLSPSFPGVSGAVNAMAAYNGNLVIAGSFTVAANVEARNIAVWN
                     GSAWSALGSGVNSSIYALVVYDGQLIVGGWFDSAGGIAANDIAVWDGTAWSTFGAGID
                     DSAVYALTVHDGELIAGGGFNTIGGVTAHKVAAWNGVSWAPLGAGIGGSYPYVNALKS
                     YNGELIAGGAFNSAGGSPAVGIAAWNGSSWRSLGTGVGGESFYVQSLAEYDSRLIAAG
                     GFTSAAGAPANNIAAWDGSRWSALGAGVSSSVYALAVYGGDLVAGGQFATAGNDTARG
                     IAAWNGSGWSPIGTGGEAYYTVSALVEFDGVLMAGGSFTSGEGRPAGGIAAWDGVTWS
                     SLSPISGFDGTVWALTVFGTDIIAAGSFTATGGAAANRIAAWNGSAWSALGDGIGGGY
                     NPHVTDVAVYDGKLIAAGQFTLAGGADAANIAAWDGAAWAPLGAGTNNRVAALEVFDG
                     RLIAAGAFTMAGGSPRSGIAAWDGGSWSALGTGLGGTSPQGQALAVFDGKLIVGGWFT
                     AAGGTPANRVAAWNGFAWQALGSGIGGPNPYVAALALWDGRLIAGGYFATAGGNPAAN
                     VAAWNGTAWSAVGSGLNSVVQTLGAFDDLLIAGGWFSSTADNLQVLDNIGAWDGAAWT
                     PLGSGTNSSIYVVQPVEQDLLVGGSFVRAGDKIAPCLAAWTKRTTCCAGIRGNANDDP
                     GDLVNVADITFLIAAVFRGGPLPECFEEGDVSGDGELKVGDITYLIAYVFRGGPAPAA
                     CP"
     gene            14222..16036
                     /locus_tag="PKM94_07780"
     CDS             14222..16036
                     /locus_tag="PKM94_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66581.1"
                     /translation="MMKSAFMFAGAAAVVLAAGNAAAQSDPFGTMDTVTLVGIGDPAS
                     GQFRIELWVYSDEDLGGTTTGFIWDNAKVQMDSAKASPLTAGAFTLGTFFYERGEIAL
                     TNANRRFLFGGLAFMDPLLGDGSGRRLWATYFFNTTGWTADDEVTFDTLFWGVGTNYS
                     YVEFNSNQEFYPTWNGAITIPPGPPTDLIMQPPRLDFAAVAGAGNPPGRTFVISSGQP
                     LAFAVLATSDWLELSPLNDTTPGTITATVDVTGLAAGTYHDTIRVTSEEATNSPLLGP
                     VTLVVSEPYSLVLSPDTLYFQGNEGGLNPLPQKFAVREEFDRVIPFTVTEEAAWLATG
                     SAGGATPDSVTVLADLAGLTAGTYIDSIAVQSSAAAETRFLFIRLELGPPLTANLVLT
                     PTSAAFVMPENPGTGVGAQDPGPGAGGLKCRDITVESDGAPLTFTVSAALPWMELRLA
                     SSVTPAILTIFPDIVGLAPGEYYDTVSVVSPQAGNSPQFIPVHVIILPDADQDEVPDM
                     YDNCPLVANPGQENSDDEGPGDACCCARCGDVDGSGSIQVTDVTYLVSHLFMGGPGCG
                     CPAHGDFDADGNIDVADVGQLIGYLYRGGRAPVSCEAR"
     gene            complement(16320..16823)
                     /locus_tag="PKM94_07785"
     CDS             complement(16320..16823)
                     /locus_tag="PKM94_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66582.1"
                     /translation="MGFKFACPNCGQEAASSFLSKGEEFLGRHCGVRNRVHAEAARTG
                     ERSSLIEAASETAGSRPSVRSSDPDWSKPWTRFWARTLDGLIGIVAMVIIVLVVSNFQ
                     PAPKDDSAGFAAILSFSLLHFCCIIPLYGFIAAAATNVVVVTPEDWTVKGRLGRGRTS
                     PIPPNAG"
     gene            complement(16928..17875)
                     /gene="meaB"
                     /locus_tag="PKM94_07790"
     CDS             complement(16928..17875)
                     /gene="meaB"
                     /locus_tag="PKM94_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOD66583.1"
                     /translation="MTVLERFRNGDVRALSRLVSFVEDQENGYRDLLGRLYASVGKSL
                     RIGVTGPPGAGKSTLVNGLTHELLRENRRVGIIAVDPTSPFTGGALLGDRVRMNEFPA
                     DGRVYFRSMATRGATGGLAAATNNVAVVYDSFGFDVTLIETVGVGQVELDIINACDVV
                     VVVMVPESGDAVQAMKAGVMEIADVFCVNKSDRPGAERIAHELRYALETRRQTEDAWK
                     VPIVLTEGLSRKGVDQLLERINAFVAFMKARGRFEQHRRSQIADQIINVLRYRFQREF
                     IDKIQSAEEFRNDLDRIFRGETDPYTVGDRLYRKYCDTH"
     gene            complement(17880..19625)
                     /gene="recJ"
                     /locus_tag="PKM94_07795"
     CDS             complement(17880..19625)
                     /gene="recJ"
                     /locus_tag="PKM94_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOD66584.1"
                     /translation="MNVAHRSKPLKWVVAVEPDPALVEHLAAATGISKNIVKILVNRE
                     IRTPEAIDRFLNPKLSDLEDPFTLKGMTEGIERVTRALFQNEKITIYGDYDVDGITAT
                     ALLYMVLNKLGAQVDFYLPNRLTEGYGLSREGIDESKNNGVSLIITVDTGITAVEEIK
                     YANSIGIDVLVTDHHEPGGDLPAAYTIINPKQIACDYKSELSGVGVAFKFCQALYRAL
                     NQDETELWEHLDLVALGTSADIVPLVNENRILTKFGINQISRTTKPGLKSLTFVAGLM
                     GKDISTGQVVFILAPRINALGRLGDAGKAIRLLATRDERAAAEIARKLDSENKRRKEI
                     DETTLEEALAQMDDVCDLEADRAIVLAQNDWHQGVIGIVASRLVERFHLPTVMISIVN
                     GEGKGSARSIPGFHLCEALKECEHLLIRYGGHKYAAGLSIAPDKVEEFRRLFVEVSNR
                     ILSEEDITPKMHIDLEIELSDIDDHFMNAIEAFAPFGPQNMRPVFLTRNCEVVGQPYV
                     VGNNHLKMRLRKGDTQFDVIGFGFGDMARMISARGCLVDAVYVVEYNTYNGVTRKQIR
                     LKDLRLTAGTGPVPL"
     gene            complement(19658..20045)
                     /gene="rnpB"
                     /locus_tag="PKM94_07800"
     ncRNA           complement(19658..20045)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM94_07800"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(20105..21571)
                     /gene="guaB"
                     /locus_tag="PKM94_07805"
     CDS             complement(20105..21571)
                     /gene="guaB"
                     /locus_tag="PKM94_07805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOD66585.1"
                     /translation="MVKISADYALTFDDVLLVPAHSTVLPKDTDVSTVIGPDLRLNIP
                     LVSAAMDTVTESALAIALARQGGLGIIHKNMSPEEQAGEVDKVKRSESGMIVDPITLP
                     PDRTIGDALEVMSRFRISGIPITEPGGRLVGILTNRDLRFQKNTQQPIGEVMTRENLV
                     TAPEGTDLETARDILHHHRIEKLLIVDDRYMLKGMITVKDIMKKILYPNACKDARGRL
                     RVGAAVGVADLEARADALVAAGVDVLCVDSSHGHSQGVLDAVRQLKARHPNIPLIAGN
                     VATGEGTDALIDAGADCVKVGIGPGSICTTRVVTGAGVPQVTAILNAVAAADRRAIPV
                     IADGGIRYSGDIAKALACGARAVMIGSLFAGTEESPGETVLYEGRSFKVYRGMGSVGA
                     MKRGSSDRYFQEHAEEASKFVPEGIEGRVPYKGALADTVYQLIGGVRAGLGICGAASL
                     EQLRARAAMVRITSAGVIESHPHSVPITKEAPNYRRIY"
     gene            complement(21616..22335)
                     /locus_tag="PKM94_07810"
     CDS             complement(21616..22335)
                     /locus_tag="PKM94_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOD66586.1"
                     /translation="MNNDIELLLKLQVIDYDLGELERSKEYIPDMMANLQREIREAQE
                     KLDAAKQQLSEAKVSQKHFEIEIKAKEGDLARYQQQMMTIKTNKEYDALVAQIDAVKN
                     EIGYREKMMLEAMERIDALEKLLPELTQRFTQVNENNSRQLAVLQEKINSIGDTVTGK
                     QSERVHLLEGIPRRIMSVYERVRKGKGGSAVVPVKKRSCSACHKALTPKLAQEIRRSD
                     TILTCENCGRLLYWDDDISDT"
     gene            complement(22532..22605)
                     /locus_tag="PKM94_07815"
     tRNA            complement(22532..22605)
                     /locus_tag="PKM94_07815"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22569..22571),aa:Val,seq:cac)
     gene            complement(22636..22709)
                     /locus_tag="PKM94_07820"
     tRNA            complement(22636..22709)
                     /locus_tag="PKM94_07820"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22673..22675),aa:Ile,seq:cat)
     gene            22939..>23597
                     /locus_tag="PKM94_07825"
     CDS             22939..>23597
                     /locus_tag="PKM94_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66587.1"
                     /translation="MRVRVGFLGVAGMLLAAPLAFASGISITTPVYGTWWNSDHLSIS
                     PGCVAEVKFRLTNTSGFDVTGFTNGFAIYTAESPLGPITNNLFYLAADTLPIGWQTNY
                     MEGVFITRRSWDLIGADTAGFSGFVINKPGIPDGFSEDVWVIRAIPDPESPFGYLCID
                     TAFFPPGGAWLWSSTNGEIVPDWSGRFCLQVEMCPCGWVVFRDTATVIDVPVNAPFAY
                     T"
BASE COUNT         3914 a         7006 c         8278 g         4399 t
ORIGIN      
        1 cgggcgtgac cggcagaccg gctctctgcg ccacctcgcg ggccgcgagt ttgtcgccct
       61 gcagggcgat ggtctcgggg gccggaccga tgaagacgag gccggccgcg accaccttgc
      121 gggcgaagtc ggcgttctcg gccaaaaagc cgtagccggg gtggatggcg tcggccccgg
      181 cgcggcgggc ggcggcgatg atgttctcat ggaccaggta gctctcggcg gccggggagg
      241 gaccgatgtg gtaggcctcg tcggccatgc gtacgtggag ggcggcgtgg tcgcagtcgg
      301 agtagacggc gaccgatccg atgccgaggt cgtggcaggc gcggatgacg cggacggcga
      361 tttcgccgcg gttggcgacg aggattttgc ggatcttgcg ggtcatggtc ctctccggct
      421 acagcggtat attcccgtgc ttcttcggcg ggttgctgtc tttcttcgtc gccagcatct
      481 cgaacgcccg gatgaggcgc gggcgggtcg tcttcggctc gatcacatcg tcgatgtagc
      541 cgcgcgaggc ggcaatgtag ggattggcga atttctcgcg gtacatctcg gtctggcgct
      601 gggtctcgag ctcggggctg gaggcgtcgg cgatctgctt gcggaagatg atctcgacgg
      661 cgcccttggg gcccatgacg gcgatctcgg ccgtcggcca ggcgtagttc atgtcgccgc
      721 ggacgtgttt ggaattcatg acgtcgtagg cgccaccgta ggctttgcgg gtgatgacgg
      781 tgactttggg cacggtggcc tcgcagtagg catagaggag tttggcgccg ttgacgatga
      841 tgccgttgtg ttcctggtcg gtgccgggca ggaaaccggg gacgtcttcg aaagtgagga
      901 tggggatgtt gaagcagtcg cagaagcgga tgaagcgcgc gcctttgcgc gaggagttga
      961 tgtcgagcac gccggcgagc acggccggct ggttggcgac aatgccgatc gagcgcccgc
     1021 cgaggtgggc gaaaccgacg atgatgttcg gggcgaagtc ctcgtggacc tcgaaaaacg
     1081 aaccctggtc gacaatgcgg gcgatgacct ctttcatgtc gtagggctgg ttggggttgt
     1141 cgggaacgag agtgttgagg agatcgtcct cgcgccccgg cgggtcggca cactcggcga
     1201 cgggcgggtc ctcgcagttg ttctggggga tgtactcgag cagccgcttg atcttcgcga
     1261 tcgtgtcggc ctcgttgtcg caggcgaagt gggcgacccc ggaccggctc gcatggaccg
     1321 ccgcgccgcc gaggtcctcg gaggtgacct cctcgtgggt gaccgtcttg accacgttgg
     1381 ggccggtaac gaacatgtag gaggtgcctt tggtcatgag ggtgaagtcg gtgatggcgg
     1441 ggctgtagac ggcgcccccg gcgcagggac cgaggatggc cgagatctgc ggcacgacgc
     1501 ccgaggcgag ggtgttgcgc aggaagatgt cggcgtagcc gccgagcgag acgacgccct
     1561 cctggatgcg ggcgccgccc gagtcgttga ggccgatgac cggggcgccg actttcatgg
     1621 ccatgtccat gaccttgcag atcttctcgg cgtgggcctc ggagagggag cctccgaaga
     1681 cggtgaaatc ctggctgaag atgtagacga ggcggccgtg gaccttgccg cagcccgtga
     1741 tgacgccgtc gccgaggatg cgctgtttgt cgaggccgaa atcgctcgag cggtgggtga
     1801 cgaacatgtc gaattcctcg aacgagttcg ggtcgcagag gagttcgatc cgctcgcggg
     1861 cggtcagctt gcccttggcg tgctgggcgt cgatgcgctc ctggccgccg ccgaggcggg
     1921 cctcgcggcg cgccgcctcc aggcgctgga gtctgtcttc aatggacatg gcgttcactc
     1981 tcgctctaga ctatgccgaa taccgcttgt tttcagtctc tttgcaccgg aagtgacaat
     2041 caatcaagcc cttccaacgc atcgtcaatg cgctcttgct cgtcgctctc ttgcctgact
     2101 ctgtctcgat actcgcgctc ttgcttcacg agcgagtccc ggtatttctg attggaagcc
     2161 gcaacgtctt gcctgatatc gtcgaaatgg tattgcctca cctcatacaa agcacatcgg
     2221 acaactatgc ggtcttcctc aacacgcgcc ttgcgccaca tcgtatgccg aggatgggcc
     2281 cgctcctcgt cgaagatgga tttccagcca gcgggaggag tgacggacag tatgaagggc
     2341 acatcataaa gcgcaagccc accaccgacg tcatcaatcc gcttaacgtt acctctatcc
     2401 acgcccaaga ccgtgatatc ggggaagtaa tgaagaacat cttctgggtg cttcactact
     2461 tgaacctggc tagctaaccc ggtcgttgcc tcagacgcct gcgggttcga tatagcttcg
     2521 ggcagttcac tgattccgat agctcttaac aggagcctaa acggctcctc gaagtctgac
     2581 gggcttcgaa agtcggcata caccttccct tggagtacag gggggatttg acaatcatca
     2641 atgacgattg gcaacactac ggtgcgccga tcctcaattt ccttgctcat gcccttgttc
     2701 atctcgacct gaacccatct ggagtcgaca gagtttcttg atagcacgac caccatgaac
     2761 ttggaatcag agatgccctt cgagactctc tcagccaagt aatcgcctac gttgatttct
     2821 ttttcatcga tccaaacatc tatcgcttgc ttggtcagct cggctgctaa tcctcttacg
     2881 aagggcttgt ctttggagct gtggcaaaga aacaccgtcg gcattatccc ctccatttat
     2941 cgatgacgtt ggcgatactg ttcaacagac ctcgccactg tccgagtccc ctccatggcc
     3001 agcgtggcca gcggcatggc cgtgtcgtgg tcgacagcga gaatgacgcc ctcggcgacg
     3061 atgcgcggca gcagcctgag cttgaccgcc atggtctcca gcgggtagag gtcggcggcc
     3121 gggacgtagg cgggtttcag gtgcgcggcc gtgggaaaca ggtcggcgta gtaccagact
     3181 ttccgcccgc cgccctcgat ctccaggccg aagtgtccct cggtgtggcc gccggtgggg
     3241 acggcgcgca ggccctcgct caggaccgtg tccccctcga tgaactgcac ctgtccggcg
     3301 tcggcgagcg cctgcaggcg ttcggggctg tagacggcgg cggtgcgctc gttggggtgc
     3361 atggcggcct cccattcgcg tcggtgggcg acgtacacgg ccccgggaaa gcgcgggacg
     3421 aagcggccgt cggcgcgccg gacggcgccg gcggcatggt cggtgtggag gtggctcagg
     3481 acgacgcggg tgacgtcggc gggggccagc ccgaggccgg ccaggccctc ctcaaggccc
     3541 gaggccgact cgacgccgta gatcttctcc tcgcgcgggg tgagggtgtc gccgaaaccg
     3601 gcgtcgaaga gcgtcacagc cccgcggccg cgcagcacaa acaggttggt caccatcgca
     3661 atccgattct cgccatccgc cggcaggagg gtctgccaga tcgccttggg gacgaccccg
     3721 aacatactcc ctccgtcgag gcgaaaccgc cgttcgacga agggagtgat gtcgcaatcg
     3781 cccaggcgca ttaccgtccg atcacattgc ccgcgatgac caggcgctgg acctcggagg
     3841 ttccctcgta gatttcgagc acgcgctggt cgcggtagaa tttctcgatg tgggagaact
     3901 cgccgatgta gccatagccg ccgtggatct gcatggcccg gtcgacacag aagcgggcgg
     3961 tctcggagca gtagagcttg gccatggagg cctccagccc gaacgattcg ccggcgtcct
     4021 gcagtttagc ggcgtggtag gtcaggtggc gggccgccgc caggcgggtg gccatgtcgg
     4081 caatcatcca ctggatcgcc tgcagcttgg cgatcggcgc gccgaaggcc tcgcgcttct
     4141 tcgcgtaggc cacggcctca tcgagcatcc gctgggcgat gccgacgccc tgggcggcca
     4201 cgccgacacg ggccgagtcg agggtggtca tggcgtagcg gaaacccttg ccgaactcgc
     4261 cgagcaggtt ctctttgggg acaaagacgt tcttgaggtg gatgccgccg gtggtcgccg
     4321 cgcgcatccc cattttctgc cggagggtgc gggcctgcca gccgggctgc ttgagatcgt
     4381 cgatgatgaa ggcggagagt ttgggcttgt ccttgacctt gcagaaaacc acggccaggt
     4441 cggccgcgtc gccgtgcatg atgaagatct tctcgccgtt gagaatgaag ccgccgtccc
     4501 cctcgaccgc ctcggtctgc tggttggcgg cgtcggaacc ggcgttcggc tcggtgagca
     4561 cgaacgcggg gatgcgcgcg gcgcgggcgc agtcgggcac atacttcttc ttctgctggt
     4621 cggaggcgaa gtggatgatc gggtgggtgg cgagttcgcc gacggcgcag aggaggccta
     4681 cggccgggtc gtggtagcag agctcctcca ccagggtgat gtactcgagg tgcgacttgc
     4741 cggaaccgcc gagttctttg ggcatggcgt agccgatgaa gccggcggcg ccgatcttgc
     4801 ggtagagctc ggtggggaag cggcgcggtt cgggcatctg gtcgagttcg cgcgtgatcg
     4861 ggtagatctc cttctcggcg aactgcttga cctggtcgcg gacagcctgt tgccgcgggt
     4921 cgatattcct gttcatgggt cctccctgac ggggttgtta tagagtgaca ttgtgcctgt
     4981 attcgcaggg ggtcatcggc gggccgccgg gtcgccgcgg gcggccggcc ctcggaaccc
     5041 ggcctacatg atctgctcgc ctttctgttt cttcgcgacc gcctcgcgga ggtactcgat
     5101 ggcggcctcg gtcggagcac cgggggtgaa gatcttgccg acgcccatat tctcgagcgc
     5161 ggctttgtcg tcctccggga tgatgccgcc gccgaagagg atgatatccc cggccccctt
     5221 cttcttcatt tcctcgagaa tcgcgggaaa gagggtcatg tgggcgccgg agaggatgga
     5281 gacgccgatc gcgtcgacat cttcctgaat ggcggcgtcg acgatcatgg gcggggtctg
     5341 gcggaggccg gtgtagataa cctccatgcc ggcgtcgcgg agggccgcgg cgatgacttt
     5401 gatgccgcgg tcatggccgt cgaggccggg tttcgcaagc aggacgcgga ttttgtctgg
     5461 catacctgaa tctctctgtc gtaaggctcg tgtagctttt ccgccgagtt cagctcggca
     5521 tcatggccgc ctgggactcg acccactccc cccactcctg ccggaggacg tcgcacatct
     5581 cccccacgct cgcgtaggcc cgggcgcact ccaggagggc ctcgaaagtg ttgccctggc
     5641 cgcgggcgac ccggcgcaga gtctcgaggc tcgcttcgac ccgggcgcgg tcgcgctcgg
     5701 ctttcacttt tttcagaaag gcgacctggt cgcgctcgat ctgcgggtcg atgcggagga
     5761 cggggatgtc gatttcctcg ttctcgagct ggaagcggtt gacgccgacg atgacgcgct
     5821 cgtcgcgttc gagttcctgc tggtgggcat aggcggcgcg ggcgatttcc tgctggaaga
     5881 agccgtcctc gatgcatttg agaacaccgc cgcgctcggc gatttctctg aagtaggcct
     5941 cggccccggc ctccatctgg tcggtgaggg cctcgacgaa gtacgagccg gcgagcgggt
     6001 cgatggtgtt ggcgacgccg gtttcgtagg cgatgatctg ctgggtgcgc agggcgatcc
     6061 gggcggcctt ctccgagggc agcgcgagcg tctcatccat cgaattcgta tggagcgact
     6121 gcgtgccgcc gaggacggcc gagagtccct ggatggcgac gcgggcgatg ttgttctcgg
     6181 gctgctgggc ggtcagcgag acgcccgcgg tctgggtgtg gaagcggagt ttccacgagc
     6241 gttcatcctg cgcccggtag cgctcgcgca ggtggcgcgc ccagatgcgg cgggcggcgc
     6301 ggtatttggc gatctcctcg aagaagtcac tgtgggcgtt gaagaagaac gacagccgcg
     6361 gcgcaaactc atcgacgccc atccccgcct cgaggcacga atcgacgtac tggaaaccgt
     6421 cggcgagagt gaacgccagc tcctgcacgg cggtcgagcc ggcctcgcga atgtggtaac
     6481 cggagatcga aatggtgttg aatttcgaaa cgtgccgcgt gcagtaggcg atcatgtcgg
     6541 ttatgaggcg gagcgacggg cgcggcggga agatgaattc cttctgcgcg atatactcct
     6601 tgaggatgtc gttctggagc gtgccggtga ccgcggcgaa cggaacgccc tgcttctcgg
     6661 ccaccgcgag gtacatcgcg aggatcatcg acgccggcga gttgatggtc atggaggtcg
     6721 agacccggcc gaggtcgatg cggtcgaaga tgatctccat atcggccagc gtgtcgacgg
     6781 ccaccccgca cttccccacc tcgccgagcg aacgggggtg gtcggaatca taccccatca
     6841 gggtgggcag gtcaaaggcg gtcgagaggc cggtgccgcc gttggcgagg atgtagtgga
     6901 aacgctcgtt ggtctgcctg ggggtgccca tgccggcgaa ctggcgcatg gtccagagct
     6961 tggtccggta cagggtgtgg tgcacgccgc gggtgtaggg atactcgccg gggaagccga
     7021 ggttgcgcca gaagtaatcc tcgcggtcgg cgcctgcgat ggaatcgggg gtgtagaggt
     7081 cgttgatgtc gacgccggag acggtgaaga aacggggcat ggatttctgg gcgcggtact
     7141 tctcggccgt ctggtcccag gtctgacggt gggccttgag ctggtcgaga aatttcttca
     7201 ggaacatggc cggctcactc cttcaggtta cagtgttcaa gactcgaaga taagcggtcg
     7261 ggcggggaaa cgcaagcggc gcgggggaaa atccgccccc ggcggcgccg ggaaagcaaa
     7321 agattctcat ggggttagcg gccgggtctc gcaaaccgcc cgtcatcgga ggagcgcacc
     7381 gcgcgaaagt ccttgccgtg tcgggccgac cggtgtatac tggaactata gggcgtatat
     7441 ctctgcgatc cagagatcgc ccggcggccg ggtcgccggc ggccgacagc acgacgcccg
     7501 acataccctt gaagcaaggg gaggtgactc atcgcaaggc cgaactgcga ttgtgtcagg
     7561 atgctgggga ttgactacgg cgatccctgg gacatcagga cattttcggg gtcctcgaga
     7621 tacctgtgga gcgagttccg tcgccaaggt gtgctcgtgg atgcattcac cccgtatccc
     7681 tcgcgcccgc tcacgatgtt ctacaaggcg cgggcgttcc acccgaacat gcggcggtgg
     7741 cgggcccggt ggcgaaggag cctgcccttt cgcaagtgcc tgtcggcgcg ggcggtcaga
     7801 aagatcctcc gaaattaccg cggcgcgttc aacgcgagcc tccagatcgg ggcgtactac
     7861 aatctcgccg ccgcgctcga ttgcccccgg gggctgctgg ccgacggcaa ctgcgctctg
     7921 tcgcaacgca cgtacgccac gtatcagaca tccgacgcca tatttcgcaa acagagggaa
     7981 ttcgagcgcg ccgtctaccg ctcgatggac cgggtgttct gtttcagccg gtttctcgcg
     8041 cagtcgatga tcgatgactt cggctgcgcg cccgagcgcg tgaaggtggt cggcgcgggg
     8101 ataaacatcg acgagacgct gatccgcaac cccgaacgaa attacggcgc caagaccatc
     8161 ctcttctccg ctttcgactg ggttcacaag ggtggaccgg tgctgctgga ggcgttcgag
     8221 aaggtgaagc gggaggcgcc ggaggcccgg ctcatcctgc tggggccgac tctcacgggg
     8281 ctgccgcaag gggtgatctg ccacggaccg ctgtccaagg atcagccgca gcaattgcag
     8341 acactcggcg aggcgttccg atccgcctcg atcttcgtgc accccacgct ggccgatgca
     8401 ttccccaatg tgattcggga ggcgatggcc gccgggttgc cgtgtgtcgc gagcgacatc
     8461 ggctccatcc cggagatggt caccgaggga cagaccggct atctggtgcc gaaagggaac
     8521 gccgcgcagc tcgcggaccg tgtgctccgg ctgctggcga atccgggttt ggcgcaagcg
     8581 atgggcgagg caggttacgc gaggtaccgg gagcggttca cctgggcacg cgtctgcgcc
     8641 accattgtcg acgagttggg gcaggtgccg gccgagcgaa gcaccggggc atggccggcg
     8701 gatcagacaa tggagacggc gttacgagat agcgccgaat gacccgccgc tttgtctctc
     8761 gacgttcgct ccgggctgtg tcatcggcgc gccgggcgag gtcggaaacg acgaccggca
     8821 atggggtaac gcgaggcgca ggccgcgcgc aggccgagac gcgggcgtca aagatgttgg
     8881 ctatccgcgc cgacactagt atattaggtg gtcttcgcgg aaaaccgggg ctgcagcgcc
     8941 agcggcgcgg ggcctctgaa tactcggaca acgccatacc caggaacacc agcgtatgcg
     9001 ggattgtatc tctctcatca ttgcagctat ccttgtggcg gccgccgccg cgccggcgaa
     9061 ctgccgcccg tcaacgccga cattcacgga gaatcgcgga cagtggcccg acagcatcct
     9121 ctttcgggcc gaggacggac cgaccgctct gtggttcacg acgggcgggg tgtactacca
     9181 gtttacgcgg gttctcggcg accgccacgc ggcgggccgc tcggctctct cgaccgacca
     9241 ggtcgcccgc aaaccggagg cggcggaact gctgacggtc aaagcggcgc tggccggagc
     9301 gaaccgtgcg gcgacggcga cgggagtgaa tccgaccgcc tcccggtgcc atttcttcct
     9361 cggcgatgac ccttcgcgct ggcggaccga ggtcgcgaat tttgcggaga tacggctgac
     9421 cgaagtgtac cctgggatcg acctggtgta ccgggggatt gcggctgatc cggggtcgcc
     9481 ggggggagcg gtcgagtatg atttccggat cgcggcgggt gcggattata cgcagatccg
     9541 ggtgcgctac gagggggccg aggggctggc gatcgacccg gagggcgggc tagtgatcaa
     9601 gaccgcctgg ggagatctcc gcgagtcgcg gccggtcgcg caccaggagg cgcacggggt
     9661 gcggtggtcg gtggaggcgg cattcgtcat caccggcccg cgggaattca gcttccgtct
     9721 gggggcaggg tacgacccct cacagccggc ggtcatcgac ccggtgctcg tgtacgggac
     9781 gtttctcggc ggaacggacc gggagcaggg caacggcgtg gccgtggaca acgcgggggc
     9841 gatctatgtg gccgggacaa cgtactcggc ggattttccg accgcggcgc catacgacgg
     9901 aacgctgggc ggggacgccg atctgtttgt aaccaagctt gcggccgacg gcagcgacct
     9961 ggtctattcc acctatctcg gggggagcgg ggcggattac ggctttgacc tggcggtgac
    10021 cggcgaggga agcgccgccg tcaccggaca gacgtactcg acggatttcc cgactgtgag
    10081 cgcctggcag accgacccgg atgtgccgga cggcgccagc gcggacgcgg tgatcgcaaa
    10141 actcgcgccg gccggcaaca gcctgacgtt cagcacgtac gccggcggaa ccaacgcgga
    10201 gtatgcctgg gggatcgcac tcggcgccga cggtgcggcc tatgtcaccg gatacacgct
    10261 ctcggacgac ctgcccacga tcaacgccat ccagacgttc cagggcggag cggacgcgtt
    10321 cgtggtgaag attccgtcgg ccggcggccc cgctctctac agcacatacc tcggcggcga
    10381 aggcgaggac tggggccgcg ggattgcggc cgacgctgcc ggggcggcct acgtgaccgg
    10441 acacacgcgg tcgacggcgt ttccgacgca aagcccgttc cagggcgacc agccggggca
    10501 cgatgcgttc gccgtgaaga tcgcggcggc cggcgggagc ctggtgtacg cgacctacct
    10561 cggggggagc gcttacgacg cggggatggg gattgcggtg gacggggcgg ggttcgcctc
    10621 cattaccggc cggacctatt cgaacaattt cccgacgcag aacgcctacc aggccaatca
    10681 ggacacgctc gatgcatttg tagtgaagct gacggcgggc gggaacggcc tcgtgtacgg
    10741 aacctacctg ggcggaaacg gactcgacga gggatgggac atcgcgctgg ggagcgacgg
    10801 cggggcatac atcaccggga acacctactc gaccaatttc ccgcagagct atgcgtacca
    10861 gggagaccag ggaacggcgg acgcctttgt cacccggctg tcccccaacg ggcggagcgc
    10921 gctctggagc acctacctgg gcggccagat gatcgacctg gggacggaca tcgcgcgcga
    10981 cagcggcggc ggagtctatg tcgcgggata cacctactcc gacgacttcc cgaccgagag
    11041 cgcataccag gcggcgcgga caggcatcga cctgttcgtc gccaagttcg atgcagcgtg
    11101 ctgcacaggg attcgcggga acgccgaggg cgacagcaac gacaacatca cggtggccga
    11161 catcactttc ctgatcgcgg cggtgttccg gggcgggccg ctgccggagt gtttcgagga
    11221 gggggatgtg agcggcgacg gcgagttgaa agtgggggac atcacctact tgatcgccta
    11281 tgtttttcgc ggcgggccgg cgccggcagc ctgtccgtga gaccttctcc cccgcgccga
    11341 tccgccggca gaatacccgg acggccgccg gccgtgacgc agaccggttt gaccttgccg
    11401 atcggtcttg gtccgatata ttgagggtac gacgggcatt ccactcggca aaacacccga
    11461 tgaagcgggg accagccgag ggcgccgcag atcagatcgc cgcgacgtcg cccattcgtt
    11521 gctgagacaa caacccgaga ccgcacctga cgaagggatc ctcatgacat tcacatcgcg
    11581 cctgattgcc cgggctgcag cggcttttct cctggcgggc ggactgctcg aagtgggcct
    11641 ggcccagtcg agaccagcgc ttccgccccc cgagccggcc ggaagcggaa cctctgacgc
    11701 cgcctttggc catcccgacg acgtcttctg ggacaacacc ctgtcgccgt cttttccggg
    11761 cgtgtccggg gcagtgaacg ccatggccgc atacaacggc aatctggtca tcgccgggag
    11821 tttcaccgtc gcggcgaacg tcgaggcgcg caatatcgcc gtctggaacg ggtcggcatg
    11881 gtcggcgctc ggctccggcg tgaattcttc aatctatgct ctcgtggtgt acgacggcca
    11941 actgatagtc ggggggtggt tcgactcggc gggcgggatc gcggcgaatg acattgccgt
    12001 ctgggacggc accgcgtggt cgactttcgg cgcggggatc gatgacagcg cggtatacgc
    12061 gctgaccgtg catgacggag agttgatcgc cggcggcggg ttcaatacga tcgggggcgt
    12121 gaccgcccac aaggtggcgg cgtggaacgg cgtctcctgg gcgccgctcg gcgccgggat
    12181 cggcgggagt tacccgtatg tcaacgcgct caagagctac aacggcgaac tcatcgccgg
    12241 cggtgcgttc aactccgccg gagggtcgcc cgcggttggg atcgccgcct ggaacggcag
    12301 ttcgtggcgc tcgctcggga cgggcgtcgg cggggagtct ttctacgtgc agtcactggc
    12361 ggagtacgac agccgactga tcgccgcggg cggcttcacc tcggccgccg gggcgccggc
    12421 gaacaacatc gccgcctggg acggaagccg gtggtcggcg ctcggcgccg gcgtgagttc
    12481 atcggtgtac gccctagcgg tgtacggcgg cgacttggtg gcggggggac agttcgccac
    12541 cgccggcaac gacacggcgc ggggcatcgc cgcgtggaac ggcagcgggt ggtcgccgat
    12601 cggaaccggc ggggaggcgt actatacggt ttccgcgctg gtcgagttcg acggcgtgct
    12661 gatggccggg ggcagtttca ccagcggcga gggccggcca gccggcggaa tcgccgcgtg
    12721 ggacggtgtc acatggtcga gcctgagccc gatttcaggc ttcgacggca ccgtgtgggc
    12781 gctgacagtc ttcgggacag acattatcgc ggccggttct ttcaccgcaa ccggaggcgc
    12841 ggcggctaac cggattgccg cttggaacgg atcggcctgg tcggcgctgg gagatggaat
    12901 cggcgggggg tacaatcccc atgtgaccga tgtagcggtg tatgacggca agctgatcgc
    12961 cgcggggcag ttcacgctcg cgggcggggc cgacgccgcc aacatcgccg cttgggacgg
    13021 tgccgcctgg gcgcccctgg gcgcggggac caacaaccgg gtcgcggcgc tggaggtttt
    13081 cgacggacgg ctgatcgccg ccggggcgtt cacaatggcc ggcggttcgc ccaggagcgg
    13141 catcgccgcc tgggacggcg gctcgtggtc cgcgctggga accgggctcg gaggcacgtc
    13201 tccgcagggc caggccctcg cggtcttcga cggcaagctc atcgtgggcg ggtggtttac
    13261 ggccgccggc gggacgccgg ccaaccgggt ggccgcctgg aacggtttcg cctggcaggc
    13321 gcttggatcc gggataggcg gaccgaaccc gtatgtcgcg gctttggcgc tgtgggacgg
    13381 caggctcatt gcgggggggt atttcgccac agcgggggga aatccggcgg ccaatgtcgc
    13441 cgcctggaac ggcacggcct ggtcggccgt cggctccggg ttgaattcgg tcgtccagac
    13501 gctcggcgcg ttcgatgacc tcttgattgc gggcgggtgg ttctcgtcga ctgccgacaa
    13561 cctgcaggtc ctggacaata tcggcgcgtg ggacggtgcg gcctggacgc cgctcgggtc
    13621 gggaacaaac agcagcatct acgtggtcca gccggtcgag caggatctcc tcgtgggcgg
    13681 gtctttcgtc cgcgccggcg acaagatcgc cccctgcctg gcagcgtgga ccaaacggac
    13741 cacctgctgc gcgggaatcc ggggcaacgc caacgatgat ccgggggatc tggtcaatgt
    13801 ggccgacatc actttcctga tcgcggcggt gttccggggc gggccgctgc cggagtgttt
    13861 cgaggagggg gatgtgagcg gcgacggcga gttgaaagtg ggggacatca cctacttgat
    13921 cgcctatgtt tttcgcggcg ggccggcgcc ggcagcctgt ccgtgagagc ggcactggaa
    13981 ccgggcttcg gaagaccggc aggattcgct tgacaccgag gttttcaggt atctccttgg
    14041 cggtaagcgg ggaacctgcc gacccatggc gattcggata tgcctgcgcg gcttgacggt
    14101 caccagggga ttaccgcggt aatctagacg cgggcaagcg gtcgcgacgc tatggggtcc
    14161 gctactcacg cctggggcgg tcttttcccc gcgcaacacc tacgtgggga aaggtcacgc
    14221 catgatgaaa tcagccttca tgttcgcagg agcggcggcc gtcgttctgg ccgccggaaa
    14281 cgctgcggcg cagagtgatc cgtttggaac catggacacg gtcacgctcg tcggtatcgg
    14341 cgatccggcg agcggccagt tccggatcga gttgtgggtc tacagcgatg aggatctcgg
    14401 cggaacgacg accggattca tttgggacaa cgcgaaggtg cagatggaca gcgccaaggc
    14461 atcgccgctg accgcaggcg ccttcacgct cggcacattt ttctatgaga ggggagagat
    14521 tgccctgacc aacgcgaacc gccggttcct cttcggcgga ctggctttca tggacccgct
    14581 gctcggcgac gggtcaggtc gccgtctctg ggctacgtat ttcttcaata cgacgggctg
    14641 gacggcagac gacgaggtaa cgttcgatac gcttttctgg ggagtcggga caaactattc
    14701 gtatgtcgag ttcaactcga accaagagtt ctatccgacg tggaacggtg cgataacgat
    14761 tccgccgggc ccgccgaccg atctgatcat gcagcccccg aggctggatt ttgcagcggt
    14821 agcgggagcc ggaaacccgc ccgggcggac attcgtgatt tcgtccgggc aaccgctggc
    14881 gttcgccgtg ttggcgacct cggactggct cgagttgtcg ccgctcaatg acacgacgcc
    14941 ggggaccatc acagcaacgg tcgatgtcac gggtttggcg gcggggacgt atcacgatac
    15001 gattcgggtg acatcggagg aggcgacgaa ttcgccgctg ctggggccgg tgacgctggt
    15061 ggtcagcgag ccgtacagcc tggtcctatc accggatact ttgtatttcc aggggaatga
    15121 aggggggctc aacccgctgc cgcagaagtt cgcagtgcgg gaagaattcg accgggtgat
    15181 cccgttcaca gtcaccgaag aggcggcatg gttggccacg ggctcggccg gcggggcaac
    15241 gccggacagc gtgaccgtgc tggcggatct cgcgggcctg acggcgggaa cctacatcga
    15301 ttcgattgcg gtccaatcat cggcggccgc ggagacccgg ttccttttca tccggctgga
    15361 gctggggccg ccgctgacgg caaatctggt gcttacgccg acctcggcgg cgttcgtgat
    15421 gccggagaac cccggcacgg gcgtcggtgc gcaggacccc ggaccgggag cgggggggct
    15481 gaagtgtcgg gatatcacgg tggagtcgga cggcgcgccg ttgacgttca ctgtcagcgc
    15541 cgctctcccc tggatggagc tgcgtttggc gtcgtcggtc acacccgcga tcctgacgat
    15601 cttccccgat attgtaggcc tggcgccggg cgaatactac gataccgtat cggtggtttc
    15661 gccgcaggcg gggaactcgc cgcagttcat cccggtgcac gtcatcatac tgcctgatgc
    15721 cgatcaggac gaggttccgg acatgtatga caactgcccg ctggtggcca accccggtca
    15781 ggagaacagc gatgacgagg gtccggggga cgcatgctgc tgcgcgcggt gcggggacgt
    15841 ggacggttcg gggagcattc aggtcacgga cgtcacgtac ctcgtgtccc atctgtttat
    15901 gggcggcccc ggatgcgggt gtccggcgca cggagatttc gatgcagacg ggaacatcga
    15961 tgttgcggat gtggggcagt tgatcggcta cctctaccgg ggcggcagag cgccggtctc
    16021 gtgcgaggcg agatgacggg cgccgacgcg atcggtgcag cgggtatgga ccagccttcg
    16081 gttgtgaagc ggtcggcccg tcggggctga ccgctctttc gtcagggatc acctgtcttc
    16141 accccgggcg aggttttcgc cggcgcaggc cctcgactcg cacgcagccc gtgggagtcc
    16201 ggaacggggc ggcgcacctc gctgcagcac acaggggaca gtgcgaaaaa tccgacaggt
    16261 cagatcagca ggctctatct attcaagtca cttcccgccg agtcccgctg cgcagacagc
    16321 tatcccgcat tgggagggat gggggaggtc ctcccgcggc ccagccggcc tttgaccgtc
    16381 caatcctcgg gcgtaactac caccacattt gtcgcggcgg cggcgatgaa tccgtagaga
    16441 gggatgatgc aacagaagtg cagcaacgag aaagacagaa tggcggcaaa accggcagaa
    16501 tcgtccttcg gcgccggctg aaagttcgac acgacaagga cgatgatgac catcgcgacg
    16561 atgcctatca gaccgtcgag agtcctggcc cagaatcgtg tccagggttt ggaccagtcc
    16621 ggatcagagg atctcaccga cgggcggctg cccgccgttt ccgacgcggc ctcgatgagc
    16681 gatgagcgct cgccggtccg agctgcttcc gcatgaaccc ggttccgcac accgcaatgg
    16741 cggccgagaa actcctctcc cttgctgagg aaactggagg ccgcctcctg gccgcaattg
    16801 ggacaagcga acttgaatcc cacaggacct cccctctcgt cgactccggg cctgctatcg
    16861 caccttcccg gcaaggatac ggcagaggcc gggcagggag gtcggcaagt gaattcgaca
    16921 ggtcgattca gtgcgtatcg cagtatttcc ggtacaggcg gtcgcccacc gtgtaggggt
    16981 cggtttcgcc gcggaaaatg cgatcgaggt cgttgcggaa ttcctcggcc gactggatct
    17041 tgtcgatgaa ctcgcgctgg aaacggtagc ggaggacgtt gatgatctgg tcggcgatct
    17101 ggctgcgccg gtgctgctcg aaccggccgc gggctttcat gaaagcgacg aaagcgttga
    17161 tgcgttcgag cagttggtcc acccccttgc ggctgagccc ttcggtgagc acgatcggga
    17221 ctttccaggc gtcctcggtc tggcggcgcg tctcgagggc gtagcggagc tcgtgagcga
    17281 tgcgctcggc gcccgggcgg tcggacttgt tgacgcagaa gacgtcggcg atctccatca
    17341 cacccgcctt catcgcctgg accgcgtcgc ccgactcggg caccatgacc accaccacca
    17401 cgtcgcaggc gttgatgatg tcgagttcga cctggccgac gccgacggtc tcgatgaggg
    17461 tgacgtcgaa gccgaagctg tcgtagacga cggcgacgtt attggtggcg gcggcgaggc
    17521 cgccggtggc gccgcgcgtc gccatcgagc gaaaatacac ccggccgtcg gccggaaact
    17581 cgttcatgcg gacgcggtcg ccgaggaggg cgccgccggt gaaaggggag gtcgggtcga
    17641 cggcgatgat gccgacgcgg cggttctccc tgagcagttc gtgggtgagg ccgttgacga
    17701 gggtcgactt gccggcgccg ggcgggccgg tgacgccgat gcggagggat ttgccgacgc
    17761 tcgcgtagag ccgcccaagc agatcgcggt agccgttctc ctgatcctcg acgaaggaga
    17821 cgaggcgcga gagggcgcgg acgtcgccgt tgcggaagcg ttcgagtacg gtcatggcgt
    17881 cacagcggca ccggcccggt gccggcggtc aggcgcaggt ctttcaggcg aatctgcttg
    17941 cgcgtgacgc cgttgtaggt gttgtactcg acgacgtaga cggcgtcgac gagacagccg
    18001 cgcgccgaga tcatgcgggc catgtcgccg aagccgaaac cgatgacgtc gaactgggtg
    18061 tcgcccttcc ggaggcgcat tttcaagtgg ttgttgccga ccacgtaggg ctggccgacc
    18121 acctcgcagt tgcgggtgag aaacaccggg cgcatgttct ggggaccgaa gggggcgaag
    18181 gcctcgatgg cgttcatgaa gtggtcgtcg atgtcggaca gctcgatttc gaggtcgatg
    18241 tgcatcttgg gcgtgatgtc ttcctcggag aggatccggt tggagacctc gacgaagagc
    18301 cgtcggaact cctcgacttt gtcgggggca atggacaggc cggcggcgta cttgtggccg
    18361 ccgtagcgga tgaggaggtg ctcgcactcc ttgagggcct cgcagaggtg gaagccgggg
    18421 atggagcggg cggagccttt gccctcgccg ttgacgatgg agatcatgac cgtggggagg
    18481 tggaagcgct cgacgagacg ggaggcgacg atgccgatga cgccctggtg ccagtcgttc
    18541 tgggcgagga cgatggcgcg gtcggcctcg aggtcgcata cgtcgtccat ctgggcgagg
    18601 gcctcctcga gggtggtttc gtcgatttct ttgcggcgct tattttcgct gtcgagtttg
    18661 cgggcgattt cggccgcggc gcgctcgtcg cgcgtcgcca gcaggcggat cgccttgccc
    18721 gcatccccca ggcggccgag ggcgttgatc cgcggagcca ggatgaagac gacctggccg
    18781 gtgctgatgt ctttgcccat gaggccggcc acgaaggtga gggatttcag tccgggtttg
    18841 gtggtgcggg agatctggtt gatgccgaac ttggtgagga tgcggttctc gttgacgagc
    18901 gggacaatgt cggcggaggt gccgagggcg acgaggtcga ggtgctccca gagttcggtt
    18961 tcatcctggt tgagggcgcg gtagagggcc tggcagaatt tgaaggcgac gccgacgccg
    19021 gagagctcgc tcttgtagtc gcaggcgatc tgcttggggt tgatgatggt gtaggccgcg
    19081 ggcaggtcgc cgcccggttc gtggtggtcg gtgacgagaa cgtcgatacc aatggagttg
    19141 gcgtatttga tctcctcgac ggcggtgatg ccggtgtcca cggtgatgat gaggctgacg
    19201 ccgttgttct tggattcgtc gatcccctcg cgcgagaggc cgtacccctc ggtcaggcgg
    19261 ttggggaggt agaagtcaac ctgggcgccg agtttgttga ggaccatgta cagcagggcg
    19321 gtggccgtga tgccgtcgac gtcatagtcc ccgtaaatgg tgatcttctc gttctggaag
    19381 agggcgcggg tgacccgttc gatcccctcg gtcatgccct tgagggtaaa cggatcctcg
    19441 aggtcggaga gcttcgggtt gaggaagcgg tcgatggcct cgggggtgcg gatttcacgg
    19501 ttgacgagga tcttgacgat atttttggaa atgccggtgg ccgcggcgag gtgctcgacc
    19561 agcgcgggat cgggttccac ggcgacgacc catttgagcg gcttggaccg gtgggccaca
    19621 ttcatcgcag cctccataag taggaattta tgaaggcttg ggaagcagga aataagccga
    19681 gttctgtcgt gaccgccgca gcggcgggac agtcatttat cttggcggtc cggttacccg
    19741 gccgctccgc gcggcccacc cgggaacata agcgggcaaa tcaaccctgt tcccctattt
    19801 ggccttgctc cgggcggggt ttgcccgccg cgtccgttac cggacgcggc cgtggtctct
    19861 tacaccgcga tttcaccctt accgggggcc gcaggcggac ccgcggccgg tcggcggtat
    19921 ggtttctgtg gcactttccg tcggatcact ccgccccggt gttaccgggc gccctgattt
    19981 ttcggagctc ggactttcct cactttaaga cacgacgttt aaagcgcgac tgtctctcct
    20041 acttccacaa gcattctatc tatatataca caccaaaaaa cgtcacgttg atccgctccg
    20101 gcggtcagta gatccgccgg tagttggggg cctccttggt gatcggcacc gagtgcgggt
    20161 gcgactcgat gacgccggcc gaggtgatcc gcaccatggc ggctctggcc cgcagttgct
    20221 ccaggctggc ggcgccgcag atgccgagtc cggcgcggac gcccccgatg agctggtaga
    20281 cggtatcggc gagggcaccc ttgtagggca cccggccctc gatcccttcg ggaacaaatt
    20341 tggaggcttc ctcggcgtgt tcctggaagt accggtcgct gctgccgcgt ttcatggcgc
    20401 cgaccgatcc catgccgcgg tacaccttga acgagcgccc ctcgtagagg acggtttcgc
    20461 cgggggattc ctcggtgccg gcgaagaggg agccgatcat gacggcgcgg gcgccgcagg
    20521 cgagagcctt ggcgatatcg ccgctgtacc ggataccgcc gtcggcgatc accgggatgg
    20581 cgcggcggtc ggccgcggcc accgcgttga gaatcgcggt gacctggggg acgccggcgc
    20641 cggtgacgac gcgggtggtg cagatactgc cggggccgat gccgactttc acgcagtcgg
    20701 cgccggcgtc gatgagagcg tccgtgccct cgccggtggc cacgttgccg gcgatgagcg
    20761 ggatgttcgg gtgccgcgcc ttgagctgcc tcaccgcatc gagcactccc tggctgtggc
    20821 cgtgggagga atcgacacag aggacgtcga ccccggcggc caccagggcg tcggcccgcg
    20881 cttccaggtc ggccacgccg acggcggcgc cgacccggag gcgcccgcgg gcgtccttgc
    20941 aggcgttcgg gtacagaatc ttcttcatga tgtctttcac cgtgatcatg cccttgagca
    21001 tgtagcggtc atcgacaatc aggagtttct cgatgcggtg gtggtggagg atgtcccggg
    21061 cggtttcgag gtccgttccc tcgggggcgg tgacgaggtt ctcccgggtc atcacctcgc
    21121 cgatcggctg ctgggtattc ttctggaagc ggaggtcgcg gttggtgagg atgccgacca
    21181 gcctcccccc cggctcggtg atggggatgc cggagatgcg gaagcgggac atgacttcaa
    21241 gagcgtcgcc gatggtccgg tcgggcggga gggtgatggg gtcgacgatc atgccggact
    21301 cggagcgctt gactttgtcg acctcgccgg cctgttcctc gggggacatg ttcttgtgga
    21361 tgatgccgag gcccccctgg cgggccaggg cgatggcgag ggcggactcg gtgaccgtgt
    21421 ccatggcggc cgagacgagc gggatgttga ggcggaggtc ggggccgatg acggtggaga
    21481 cgtcggtgtc tttgggcagg acggtcgagt gggccgggac cagcaggacg tcgtcgaaag
    21541 tcagggcgta gtcagcggat attttcacca tagcaggccg tgatcgtttt cctcccggcg
    21601 cgcgcgccgg gtgtttcagg tatccgaaat gtcgtcatcc cagtacagga ggcggccgca
    21661 gttctcgcag gtgaggatgg tgtcggagcg gcggatctcc tgggccagtt tgggcgtcag
    21721 ggccttgtgg caggcgctgc acgaccgttt cttgaccggg accacggccg agccgccttt
    21781 gcccttgcgg acccgctcgt agaccgacat gatccggcgc gggatgccct cgaggagatg
    21841 gacgcgttcg gactgcttgc cggtcacggt gtcgccgatc gagttgatct tttcctgcag
    21901 aaccgccagc tggcgggagt tgttctcgtt aacttgggtg aatcgctggg tgagttcggg
    21961 gaggagtttt tcgagggcgt cgatgcgctc catcgcctcc agcatcattt tctcgcggta
    22021 gccgatctcg ttcttgacgg cgtcgatctg ggccacgagg gcatcgtact ccttgttggt
    22081 cttgatcgtc atcatctgct gctggtaccg ggcgaggtcc ccttccttgg ccttgatctc
    22141 gatttcgaag tgtttttggg agaccttggc ctccgagagc tgctgtttgg cggcatcgag
    22201 cttctcctgt gcttcgcgaa tttcccgctg gaggttggcc atcatgtcgg ggatatattc
    22261 tttggagcgc tccagttcgc cgagatcgta gtcgatcacc tgaagcttca gaagcagctc
    22321 gatgtcgtta ttcatatgaa cggactcctt ggcggactga gaatggttca tgggaaactc
    22381 cataatgaaa aagcgcatcc gccggatggg ccggtcgaat gcgcctggtc ttcacgtcgg
    22441 gggatttcgc ccgcccgcgc acccaggggg caggcgagga atccatatgt gcgagtcgcg
    22501 gagtgatacg ccattcctct gtcaaagaca atgggcaata ctggactcga accagtgacc
    22561 tctcggatgt gaaccgaacg ctctaaccaa ctgagctaat tgcccatttc aaatctgact
    22621 tcctcaccaa tctcgtgggc cctgcagggt tcgaacctgc gacccgctga ttatgagtca
    22681 gctgctctaa ccaactgagc taagggcccc gttgggctta acttataatt atacagtcgg
    22741 ctcccgcacc cgtcaagtcc atattgcgaa taatttgccc ccgcgcggcc gcctaagccg
    22801 ttgcgactga tgggccaagc cggcggcgcc ggaaaacgcc ttgacagatt ctgtcccagg
    22861 gctatactaa aagtagacaa gccataacac ctttgaccgt ctctgcgccg cagagggcag
    22921 actcaccgga gggcactcat gagagtgcga gttggattcc ttggggtggc agggatgctg
    22981 ctggcggcgc cgctggcctt cgccagcggt atcagcatca ctaccccggt ttacggcacg
    23041 tggtggaaca gcgaccattt atccatctcc cccggatgtg tggccgaggt gaagttccgc
    23101 ctcaccaaca ccagcggatt tgacgtcacg ggtttcacca acggcttcgc gatttacacc
    23161 gccgagagcc cgctcggccc gatcacgaac aacctgttct acctggccgc cgacacgctg
    23221 ccgattggtt ggcagacgaa ctacatggag ggggtgttca tcacccgccg cagttgggat
    23281 ctgatcgggg cggatacggc cggattctcc gggttcgtca tcaacaaacc cgggattccc
    23341 gacggtttca gcgaggacgt atgggtgatc cgggcgatcc ctgatccgga aagcccgttc
    23401 ggctatctct gcatcgacac cgccttcttt ccccccggcg gggcctggct ctggagttcg
    23461 accaacggcg agatcgtgcc cgactggagc gggcgcttct gtctccaggt cgagatgtgt
    23521 ccctgcgggt gggtggtctt ccgggacacc gccaccgtca tagacgtgcc ggtcaacgcg
    23581 ccgtttgcct atacctt
//
LOCUS       DAOPOE010000048        22648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2840_length_22648_cov_4.600673, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000048 DAOPOE010000000
VERSION     DAOPOE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22648
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_22648_cov_4.600673"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKM94_07830"
     CDS             complement(<1..348)
                     /locus_tag="PKM94_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD66588.1"
                     /translation="MIDSHCHLDFAAFEGRREEALADARAAGVHTVINIGVDLAASER
                     SVALAEQHAMVYATVGVHPHDATTLDADTLARLRALARHEKVVAVGEIGLDFFRDRSP
                     RETQKRAFHQQLEL"
     gene            complement(348..2276)
                     /gene="metG"
                     /locus_tag="PKM94_07835"
     CDS             complement(348..2276)
                     /gene="metG"
                     /locus_tag="PKM94_07835"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD66589.1"
                     /translation="MAKPIYLTTPIYYVNDRPHIGHAYTTIAADLLARFHRLAGREAF
                     FLTGTDEHGTKVAEAAAAAGMTPQAFCDRTVETFRRAWENLAIDYDYFIRTTSPRHAQ
                     AVHRLLDAMRAARTDDGREAVYSDYYEGLYCTGCEKFITEKELVDGLCPDHKRPPEKL
                     REKNYFFRLSAYLPKIREKILSGELRILPEERRREVLGLIEQDLGDFSISRERVKWGI
                     PLSFDRTQVAYVWVDALSNYISAVGYGDDPAAFAKWWTNAEVVHLMAKDILKFHCLYW
                     PAMLMAAGVRLPDTMFLHGFFTVDGEKMSKSLGNQIDPNAMVAQFGADGTRYLLLTQY
                     PFGVDGDIQAQRFAAQYNADLANDLGNLVSRVVKMVQTNFEGRLPGPHREIAGLQELM
                     EAAEAAPAAAYEHIRNFRIGQAVAEGMNLVRAANKFFNDRVPWQLARDGRRDEMGGIL
                     YACCEVIRIVSIILAPVMPNKMRAVRGIFGLDDGTLTLDAARVFFALEPGTPVAFGEA
                     VFPRIAPPQEAPPATAAAPPPAEGSADGLIDIADFGRVQLRVAEVLRAERVPGADRLL
                     RLQISLGDETRQIVAGVAEHYTPEQITGRRIVVVANLKPATIRGLESRGMLLAAKKGK
                     VLRLVVPDGDLPPGAAIS"
     gene            complement(2298..3197)
                     /gene="ricT"
                     /locus_tag="PKM94_07840"
     CDS             complement(2298..3197)
                     /gene="ricT"
                     /locus_tag="PKM94_07840"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOD66590.1"
                     /translation="MAELYLVEFKGSRKEFFFNRYYHALTLNSYVIVEVERGEELGYL
                     KKKIETEVSFGEGNHPRSILRPAGSEDAARWEYLRDQEEGYKYEVIDIISRHGLPMKV
                     VDVECQFDGNKMTVFFTADHRVDFRELVKDLAARYRTRIELRQIGVRDEARRLGGYGI
                     CGREQCCNSFIRNFEPISTQHAREQDLPLNPSKISGNCGRLLCCLRYEADMYRKVKSR
                     FPEPGTMVRTRLGEGVIERIDVFNEEAIIRDAERVTFRVGLKDILERERKVEEAAAGP
                     ARDLEAGETEAEALKKLDEGEGR"
     gene            complement(3239..4357)
                     /locus_tag="PKM94_07845"
     CDS             complement(3239..4357)
                     /locus_tag="PKM94_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66591.1"
                     /translation="MPFDISQFQPRAWEVMARSVESDRVAGTYLLCGPDGCGRWGLAV
                     SFAALLNCEARTRTAGGALRPCGGCRNCRLIFALNFEGLAVAAPLPPHKGEDEAVELT
                     NEVIDLKRREPFAVLSATANLTIPIDTARRIKRTMATKPPPGVYRTIIFYQMERMRPE
                     AADALLKMIEEPPSQAVILLIAEKAERLLPTIQSRSRKVTLRSSPPEVVAEYLRAQYG
                     LAEARAELAARTAEGIAGRAVQAAESGDDEASGRVTAFQLFRSLFEDSGPETVARLTA
                     ALGERDRGEAEYLLHLWQSLARDCMQLAVAGAGGAVVNTDFRAELERLAEHFADPATA
                     VRLAEEIKIALAHFRLNVHIHGALAALALRTKAAASRH"
     gene            complement(4347..5522)
                     /locus_tag="PKM94_07850"
     CDS             complement(4347..5522)
                     /locus_tag="PKM94_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD66592.1"
                     /translation="MRRAAWAAALLLVLGAADVQADKGKDKDRIRSQQEELERIRKDV
                     ERSEKKLDSLRSAELQTTEQISRYDQKITANKKVIGRLTRQLADVHEQMQATEDRLGQ
                     SRDRLERARRKYLGDIRQFYAKATGRTARPLWSGPAKGLEENRQIVYLAALAQFESDN
                     VADAGAYLSETLAMLDTLASQRTKVQSLKKDKETATALDLSQREKREKALEKIRRTKL
                     AEGDKMLTLQQAADEIERVIALLEEQRERRESRPSEPAPPSAFATLKGQLPPPAPGKV
                     TVPFGPAQDPVTKLKSYSPGITIAAAPGAAVRAVAAGSVAYVGTLRGYGRFVIISHDS
                     DYYTTYAGLGEPQVRTGDAVTGTTVLGRVGPEGQVKFELRQGREPLDPVLWIRLDAF"
     gene            complement(5519..6376)
                     /locus_tag="PKM94_07855"
     CDS             complement(5519..6376)
                     /locus_tag="PKM94_07855"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOD66593.1"
                     /translation="MTRTAHIAGEFARSLGRHPFTALGSLLSLTLLYLLFDLYWIGAA
                     TVEQVFARAVAEVQMEVFVREEVAEGEVAGVQAEVAAIPGVTGLQYISKEAAREIMQA
                     SLGMDLLAGYDTLNPLPRSFVLTFAPAALTSDGLAAIAGELRRMPAVADLYYNAEWLR
                     KTEGTQAVILRVGLALGILILVTAVISSLNNLRLMIRTRAEGLAQMRLLGASRLFLVA
                     PMLLEGAVLGGAAAAIGWAALWYARGQVSFTHVAPVFPPLAEIAGFCLGAALLGVVSG
                     YLGVRRMLA"
     gene            complement(6373..7038)
                     /locus_tag="PKM94_07860"
     CDS             complement(6373..7038)
                     /locus_tag="PKM94_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD66594.1"
                     /translation="MPDTAIVELSNVDLAYPRSGELFRNLSLSVRPGETAVITGGPGS
                     GKTTIAELLVGRKFADAGSVEVFGENLRRGRGRALNRVRRRLGGVGGIFGLVPTYTAA
                     DNITYPLVLAGVRKRARKERLLRMLTEFSLLTRAQDYPATLTRVEQTLVQLARASIAD
                     QPLLVIDEPMAGLDTATARRVFEYLVKASLSGRTMVLLMSDHPPGTLPNSTSYVIREG
                     RLT"
     gene            7170..8117
                     /locus_tag="PKM94_07865"
     CDS             7170..8117
                     /locus_tag="PKM94_07865"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOD66595.1"
                     /translation="MRKYLLTPLVLSLCLSAPRAQDWVPVTLPTGDNITGIEFLHPDT
                     GFLSIQPGDLGRTYDGGRTWTIHPVALDLALEDVSFANAKLGLVCGKAGALYRTSDGG
                     ERWESVSPVGRDSTVAFFSVKFIDPRTALVTGMSRHEETPWSGIAYRSLDGGATWTRL
                     EPMGLMYSEIFYSPTAGLVFPALGLLNRSDDKGKSWRQNGAIESALNRSVAVAGDLIV
                     LVGPKGFLAVSRDNGKTWKKIEQDVERTFVAVTAIDDRTVFVAGPNSLMLRSSDGGET
                     WTQELLARSFDVLDLARAGSRLYAVGTDGAVIYKELGRK"
     gene            complement(8191..>9736)
                     /locus_tag="PKM94_07870"
     CDS             complement(8191..>9736)
                     /locus_tag="PKM94_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD66596.1"
                     /translation="IRNCVFQGDLQAGIVAEFAYVKVVDCRFLGVQQGVACDHGRNVR
                     MEGCTFYGCGFAGGGHGSYIDVRRCIAAYGGMTFSSDECGGYGGQWVECSDLYGNTVD
                     WPPAIERYRTWLGNMTRPPIFCDTAAGNLDIDELSPCAPESPFNTCGVLIGAGQPACR
                     VCDDGDGDGYCAELDNCPAAYNPAQTDSDGDGSGDLCDVCPEDSLNDGDGDGLCAQED
                     NCPLAHNPGQEDEDVDGAGDACDNCLGLPNPEQGDLDRDGTGDACDACTDSDGDGYGN
                     PGYAANECPDDNCPELSNPDQTDVDGDGIGDACDWCIDSDGDGFADPGYSTEYCGADN
                     CPGVANADQADGDGDGVGDVCDNCPAAGNPGQADGDGDGIGDACDNCPALPNAEQANA
                     DGDTLGDLCDACPNDPDNDADEDLFCADADNCPAVPNPDQADFDGDGVGDACCCMLRG
                     DCTGDGTVAVSDLTFAIAYLFRGGVSAGCPAHMDVNGDGSPAVSDLTLLIGYLFRGGP
                     VPAACP"
     assembly_gap    9737..9746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9747..10741)
                     /locus_tag="PKM94_07875"
     CDS             complement(<9747..10741)
                     /locus_tag="PKM94_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66597.1"
                     /translation="MRSRLLRAVVRCLLAVALAIAGAAGGLQADPSGSSPEPISRPAA
                     DLSDTQAGGGRYECPGCDYVSYCGSAAYIYRLPSRSAATRAAVRFDVASFGNPGELAL
                     GAVQLALYPSLMTGQPGLRVYVYADNGSGYPGALLDSTEVPYDSLPTTLAWVTVPLGA
                     LDVGFEMDQSFHIGLGAIGGDGDTLVLVTDSGDGPSAGEHRSTVYDGDQWESLYALSG
                     GQADYCFLVQVSRCDPTQLPTALYVPSEYPTIAEALAAAHYCDTVVIEDGIYTIDSTN
                     WMVWNRMPMMVRSANGPEHTIVQVALANYPPAILIQASDTLPTVEGLGFRAAPGW"
     gene            10905..11390
                     /locus_tag="PKM94_07880"
     CDS             10905..11390
                     /locus_tag="PKM94_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66598.1"
                     /translation="MKHALAILIGLLLCLAVSAPLAQEDAKPWFDLEKCAFCKQLLSE
                     EGLIDHMHPAYHNLKSGVLWITVVDPEYRDAFKRAGAKMEEVGAEMQKTGEVPYMCGF
                     CSAYGELMMAGAVPECVETEVAEICLMQSDKPELVERLHAFAARSSEEMAKLKAAESA
                     E"
     gene            complement(11497..11937)
                     /locus_tag="PKM94_07885"
     CDS             complement(11497..11937)
                     /locus_tag="PKM94_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66599.1"
                     /translation="MGRVRVSIPEPAVSRASLLRGAGVAWAIGAAVLLWRAGILTRGL
                     PGGSAWVWGAAAVLGVAKYRLALRPVARRNVARIRELSPHKDKICLFAFQSLQSYVIV
                     AAMVGAGIALRHAGVPPIVLVAVYVVIGVALALGAGMHFREAGR"
     gene            complement(12004..13251)
                     /gene="rho"
                     /locus_tag="PKM94_07890"
     CDS             complement(12004..13251)
                     /gene="rho"
                     /locus_tag="PKM94_07890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOD66600.1"
                     /translation="MEVADLKQKTIADLLKIAEELDIPGVSGLRKSELIYKIMESTSS
                     GEGMILAEGVLEILEEGYGFLRSPSYNYLPGQDDIYVSPSQIKRFDLRTGDVISGQVR
                     PPKDNERYFALLKIESVNFDDPEVAKHKTLFDNLTPLYPNVQFHLEVGAEEVTTRIID
                     LMCPIGKGQRALITSPPKAGKTIILQKIAQAITANHPEVILIVLLIDERPEEVTDMKR
                     SVRGEVVSSTFDEPAERHVQVAHMVLEKAKRLTEYGKDVVILLDSITRLARAHNAVVP
                     HSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIETGSRMDEVIFEEFKG
                     TGNMEMVLDRRLSDRRIFPAMDINRSSTRKEELLQDQDTLNKIWILRKFLAEMNPIEA
                     MEFLLGRLKKTKNNAKFLASMKD"
     gene            complement(13303..13638)
                     /gene="rsfS"
                     /locus_tag="PKM94_07895"
     CDS             complement(13303..13638)
                     /gene="rsfS"
                     /locus_tag="PKM94_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOD66601.1"
                     /translation="MALAAGYLALSKKGFDVKILKLKEISSVCDYFVIASGDADVQVK
                     AIARAIEDGLREQGIKPYQVEGAEAGNWVLIDYVDVVIHVFYEPTRRFYQLERLWGDA
                     PAEELADAV"
     gene            complement(13659..14126)
                     /locus_tag="PKM94_07900"
     CDS             complement(13659..14126)
                     /locus_tag="PKM94_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66602.1"
                     /translation="MIRKFTTIFLTAGLAAVTIYLGLVGTRLVRGVSLTENPPSEVVR
                     LQVLQTGGSGAGLNRLVQRVKALAGADLEVQVVETGVYDLRAVTRTVVVSRTEDLEGA
                     GMLAEQLGLDDDAVWFQPLEHNTRQVTATLILGPDIEDLLSAQEIKKESQQQS"
     gene            complement(14247..14729)
                     /locus_tag="PKM94_07905"
     CDS             complement(14247..14729)
                     /locus_tag="PKM94_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66603.1"
                     /translation="MHKLIVILATLTLAASAAAQMSDRADLEAAARANVMGIEPAKSP
                     LSLIDLSRLHWSHSYSLSYLSGGGQSASVGMLTTSMFYEFSPKLSLLLNVGVAHNAGA
                     LWGEGVSDATVLPGFMLDYHPSESFRMTVGMQSYTGGWYGPYYRRGSLFGPGSWWNRY
                     "
     gene            complement(14869..15633)
                     /locus_tag="PKM94_07910"
     CDS             complement(14869..15633)
                     /locus_tag="PKM94_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOD66604.1"
                     /translation="MPETRAAIVLAAGKGKRMKSDLPKVLHEIDGRPMVRILLDTLLP
                     MRFERIVVVIGHKGEMVQEALRGCPVEFAWQREQLGTGHAVMMTKELLGDFAGTTLVA
                     AGDVPFLSRRSLEALLDVHSRTGAAATCLSAVFANPYGYGRIVREGNTDMLAEIVEEK
                     DASDEVRRIREINTGILCFDNRHLFAALTEIGRENAQGEYYLTDTIKVMHDKGLRVAV
                     VTAENPDEVLGVNSVEQLAELARKFGYPAAGSGKYQ"
     gene            complement(15691..16044)
                     /locus_tag="PKM94_07915"
     CDS             complement(15691..16044)
                     /locus_tag="PKM94_07915"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOD66605.1"
                     /translation="MLITVCKSKIHRATITQCSLDYVGSMTIDEQLMKAADLVEYEKI
                     QVANVTNGERFETYVIKGKPGSGTIALNGAAAHKGAVGDLVIIIAYGLIDHKDGSVHK
                     PAVVHVDQNNKQIRP"
     gene            complement(16217..16693)
                     /locus_tag="PKM94_07920"
     CDS             complement(16217..16693)
                     /locus_tag="PKM94_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD66606.1"
                     /translation="MNESTKIPVESPGVNVAVVKKDPDGWKFLVLQWSAEDAYGGYWG
                     FVNGGKQGGETVAQVVVRKLKVELGLNPLSMWATEYVVHFYEPVYDKVLILPLIVAVV
                     DPESQVKLSPEVSSFRWLPPGKARHLVSWKNLERMIEEITDELEIFPARNWVQITA"
     gene            16907..17458
                     /locus_tag="PKM94_07925"
     CDS             16907..17458
                     /locus_tag="PKM94_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD66607.1"
                     /translation="MTTTPGRKDNRPASAAVETLIGVYAAAPARLSGALAGLSDHELA
                     ARPIDDKWSIQEIVFHLADSELMAAARFRQAISGSHPTFAYYSQDIWAEKFDYRHADR
                     RALEEAVDLFAALRAATTRLLRRLSGDQWALTGHHPERGPLSVRQILETYANHGENHV
                     KQILERRRLLGKPLDLAPLTPGA"
     gene            complement(17544..18389)
                     /gene="panC"
                     /locus_tag="PKM94_07930"
     CDS             complement(17544..18389)
                     /gene="panC"
                     /locus_tag="PKM94_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOD66608.1"
                     /translation="MRIIRSIRQMQRAAREAAAAGKTIGVVPTMGYLHEGHLALVRRA
                     KKAADLVVVTIFVNPAQFAPGEDFARYPRDEKGDVKKIRSAGGDLVFIPSAADMYPPG
                     FQTYVTVGGVTEALEGAVRPGHFLGVATVVAKLFNIVRPDVAVFGMKDYQQAVVLRQV
                     ARDLDYPIRMVIAPTVRESDGLAMSSRNAYFKEPQHRAEARCLYYALRTARAMAKAGA
                     VDPKKIRAEMEKVIHGACPSAEIDYIAFTDLWSVQPAAAVRPGVVCSLAVRVHGVRLI
                     DNMRM"
     gene            18952..20808
                     /locus_tag="PKM94_07935"
     CDS             18952..20808
                     /locus_tag="PKM94_07935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD66609.1"
                     /translation="MFDALRRMILPIILIVLLLFAGMIVLEWGMGLSGRQRFADANVA
                     GVVNGEEISWERYNTILNNMMQSEQQDSDEDLPDAKVRELQLEAWKQVVQDQIMQQKV
                     RDEAIVVTDEELFSYLAYSPPPELRTLPYFLTNGQFDYQKYQQAMLDPQAAPFWSQIE
                     AAARNDIAKMKVQELVLQDVQVTENEVREWFLGSQEKVTVGMINVDFARFSRPAPTGT
                     AEEIEAYFNAHRDKYTVEERAALNVVLIEKSPAPSDWEASYERARAIYDSIKAGTDFA
                     EMAGLYSEDPGSKEKGGDLGWFARGQMVGEFEKVAFQLNKDQVSEPVKTQFGWHIIKL
                     FDRKTEREVPRGKTEPEDVEKVLASHILIQAKPSQETLDQAYRRLEAFRTDAKKIGFF
                     KAAEDHQFPVRNTGLFFRGRNIQYLGSDAQAGMFAFDEAVDAISDVWENNSAIYVVQV
                     AEKRPAGLATFEEARERVTLDVQQEKVMALCVDTAAAIWNLIQQGSDPKTAAERFGEQ
                     YETPDPFTRSGYAPGIRRDPLAIGAAFGLSRPGEFSGPVKYDQGVVIFRLLSKEAPDM
                     SVYTEKRDSVRTVVLNNKRQELFTRWMEETVKSSKVENYVQRALVEQRGMPY"
     gene            complement(20831..21097)
                     /locus_tag="PKM94_07940"
     CDS             complement(20831..21097)
                     /locus_tag="PKM94_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66610.1"
                     /translation="MTALVPGSAGVALLLAAFVLSLLGRLSEHGIAYLAMNALGAALA
                     CWYAWASGLLPFVVLEGAWAAAALVRLAVGTGKKRGSPQTGEPR"
     gene            complement(21122..>22648)
                     /locus_tag="PKM94_07945"
     CDS             complement(21122..>22648)
                     /locus_tag="PKM94_07945"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOD66611.1"
                     /translation="YAGAHSLSGAVMDPRGIRELIPDFETLGAPLAAKVGADSLYWLS
                     ERGATKFPFQPPALSNHGNYIISLNKFVGWLAEQVEGAGVDIYAGLAGYQLLVENGAV
                     TGLQTVDLGVDKHGNPKSNFEPGSIIRAKVTVLCEGVRGSLTRQAFEKIPALTAGAVP
                     QAYLTGVKEVWEVPAGRIAAGTVIHTIGWPQPRHEYGGGWVYAMSDTMVSVGYAVGLN
                     SPDPTNDPHMKFQRYKTHPFIRRLLDGGQMQHYGAKAIPDNGCFAMPKLYHDGLLLCG
                     DGAGMLDPARLKGIHLAIKGGMLAAETLFECAKSGDYSTARLKAYAERFERSWAGAEL
                     RKTRNFHAGFEGGLYAGMFHAAVQTVTGGRGLFDRRVKKPDHEYMLTLAGFERRFGRR
                     PEKARVKCDNAYTFDKLTDVYKSGTMHEEEQPPHLVIADYDICNNRCPEEYGNPCQHF
                     CPAQVYEMVDDDQRPGKVRLQLTPSNCVHCKTCDIADPYQIIRWMTPQGGEGPNYENL
                     "
BASE COUNT         3716 a         7996 c         6971 g         3955 t
ORIGIN      
        1 cagctcaagc tgctggtgga aggcccgttt ctgcgtctcg cggggcgagc ggtcgcggaa
       61 gaaatccagc ccgatctcgc cgaccgccac cactttttcg tgccgggcca gcgcgcgcag
      121 gcgcgccagc gtgtccgcgt ccagcgtcgt ggcgtcgtgc ggatgcactc ccacggtcgc
      181 gtacaccatg gcgtgctgct cggccagcgc gaccgagcgc tccgaggcgg ccagatccac
      241 cccgatgttg atgacagtgt ggacgccggc ggcccgcgcg tcggccagcg cctcctcgcg
      301 ccggccctcg aaagccgcga aatcgagatg gcagtgggag tcgatcatca gcttatcgcg
      361 gcgcccggcg gaagatcccc gtcgggcacg accagccgaa gcactttccc ctttttcgcc
      421 gccagcagca tcccccgcga ctccagcccc cggatggtcg cgggcttgag gttggcgacg
      481 accacgatgc gcctgcccgt gatctgctcc ggcgtgtagt gctccgccac cccggcgaca
      541 atctggcggg tctcgtcgcc gagtgaaatc tgcaggcgga ggagccggtc cgctccgggg
      601 acccgctcgg cgcggagcac ctcggccacc cggagctgca cgcggccgaa gtcggcgata
      661 tcgatgaggc cgtcggccga gccttccgcg ggcggcggcg cggccgcggt cgcgggcggc
      721 gcttcctgcg gcggcgcgat ccgcgggaag accgcctcgc cgaaggccac tggcgttccc
      781 ggctccagcg cgaaaaacac ccgcgcggcg tcgagcgtga gcgtcccgtc gtccagtccg
      841 aaaatgccgc gcaccgcgcg catcttgttg ggcatcaccg gggcgaggat gatcgagaca
      901 atccggatca cctcgcaaca ggcgtagagg atcccgccca tctcgtcgcg ccgcccgtcg
      961 cgggcaagct gccacggcac gcggtcgttg aagaacttgt tggccgcccg cacgaggttc
     1021 attccctcgg cgacagcctg cccgatccgg aaattccgga tgtgctcgta ggccgcggcc
     1081 ggcgcggcct cggccgcctc catcagctcc tgcagcccgg caatctcccg gtgcggaccc
     1141 ggcagccggc cctcgaaatt cgtctgcacc atcttgacca cccgcgacac gaggttcccc
     1201 agatcgttcg cgaggtcggc gttgtactgc gccgcaaacc gctgggcctg gatgtccccg
     1261 tcgaccccga acgggtactg agtgagcagc aggtaccggg tgccgtcggc cccgaactgg
     1321 gcgaccatgg cgttcgggtc gatctggttc ccgagcgact tcgacatctt ctcgccgtcg
     1381 accgtgaaaa acccgtgcag gaacatcgtg tccggcaacc gcactcccgc cgccatcagc
     1441 atcgccggcc aatagaggca gtggaacttg aggatgtcct tggccatcag gtgcacgact
     1501 tcagcgttgg tccaccactt cgcgaacgcc gccggatcgt cgccgtaccc gaccgccgag
     1561 atgtagttcg acagcgcgtc cacccacacg taggccacct gcgtgcggtc gaacgacagc
     1621 gggattcccc acttcacccg ttcgcgggag atcgagaaat ctcccagatc ctgctcgatc
     1681 aggccgagca cctcgcggcg gcgctcctcc ggcaaaatcc gcagctcgcc cgagaggatc
     1741 ttctcgcgga tcttcggcag gtaggcggac aggcggaaga agtagttctt ctcgcggagc
     1801 ttctccggcg ggcgcttgtg gtcggggcag aggccgtcga ccagctcctt ctcggtgatg
     1861 aacttctcgc agccggtgca gtagagcccc tcgtagtaat cggagtagac ggcctcgcgg
     1921 ccgtcgtcgg tgcgcgccgc gcgcatggcg tcgagcagtc tgtgcacggc ctgggcgtgg
     1981 cgcggcgagg tagtgcggat gaagtagtcg tagtcgatcg cgaggttctc ccaggcgcgc
     2041 cggaacgtct ccaccgtccg gtcgcagaac gcctggggcg tcatgccggc ggcggcggcc
     2101 gcctcggcca ccttggtccc gtgttcgtcg gtgccggtga ggaaaaacgc ctcgcgcccg
     2161 gccaggcggt gaaagcgcgc gagcaggtcg gccgcgatcg tggtgtaggc gtgcccgatg
     2221 tgcgggcggt cgttgacgta gtaaatgggc gtcgtgaggt aaatcggctt ggccacagct
     2281 ggtctccgcg ttcgggttta ccgcccctcg ccttcgtcga gcttcttgag cgcctcggct
     2341 tccgtctccc cggcctcgag gtcgcgcgcc gggccggctg ccgcctcctc cactttgcgc
     2401 tcccgctcca gaatatcctt caacccgacc cggaacgtca cccgctccgc atcgcgtatg
     2461 attgcctcct cgttgaacac gtcgatccgc tcgatcacgc cttcccccag ccgcgtccgc
     2521 accatcgttc ccggctcggg gaaccggctc tttactttgc ggtacatgtc ggcctcgtag
     2581 cgcaggcagc acaggagccg cccgcagttg ccggagattt tcgaggggtt cagggggaga
     2641 tcctgttccc gcgcgtgctg ggtcgaaatc ggttcgaaat tgcggatgaa ggaattgcag
     2701 cactgctccc ggccgcagat gccgtagccg cccagccgcc gcgcctcgtc gcgcacgccg
     2761 atctggcgca gctcgatccg cgtccggtag cgggccgcca ggtccttgac cagctcgcgg
     2821 aaatccaccc ggtggtcggc cgtgaaaaac accgtcatct tgttcccgtc gaactggcac
     2881 tctacgtcca ccaccttcat cggcagtcca tgccgggaga tgatgtcgat aacctcgtac
     2941 ttgtacccct cttcctggtc gcggagatat tcccaccggg ccgcgtcttc actgccggcc
     3001 ggccggagga tcgagcgggg gtggttcccc tccccgaacg agacctcggt ctcgattttc
     3061 ttcttgaggt agccgagctc ctctccgcgc tcgacctcga cgatcacgta gctgttgagg
     3121 gtgagcgcgt ggtagtagcg attgaagaag aactccttgc gggagccctt gaattccacg
     3181 aggtacagtt ccgccataca aaaaatcccc gcttgcccgg ggggtgcccg ctttccggtc
     3241 aatgccgact cgccgccgcc ttggtcctga gggccagcgc cgccagcgcc ccatgaatat
     3301 gtacgttcag ccgaaaatgg gcaagcgcta tcttgatctc ctcggcgagg cgaacggcgg
     3361 tcgccggatc ggcgaagtgt tcggccagcc gctccagttc ggcccggaaa tcggtgttca
     3421 ccacggcgcc gcccgctccg gccaccgcca gctgcatgca gtcccgggcc agcgactgcc
     3481 acaggtggag caggtactcc gcctcgccgc ggtcgcgctc tcccagcgcg gccgtcagcc
     3541 gcgccacggt ctccggcccg ctgtcctcaa acagcgaccg gaacaactgg aatgcggtca
     3601 cccgtcccga ggcctcgtcg tcgcccgact cggccgcctg caccgcccgg ccggcaatcc
     3661 cctcggccgt gcgcgcggcc agctcggcgc gcgcctccgc cagaccgtac tgcgcccgca
     3721 ggtactccgc gaccacctcg gggggagacg aacgcagcgt cactttccgc gaccgcgact
     3781 ggatcgtcgg cagcagccgc tcggctttct ccgcgatgag gaggatgacc gcctgcgacg
     3841 gcggttcctc gatcattttc aaaagcgcgt ccgccgcctc cgggcgcatc cgctccatct
     3901 ggtagaagat gatggtccgg tagacgccgg gcggcggttt ggtcgccatc gtccgcttga
     3961 tccggcgcgc ggtatcgatc gggatcgtca gattggccgt agccgagagc accgcgaacg
     4021 gttcccggcg cttgaggtcg atcacctcgt tggtcagctc gaccgcctcg tcctcgccct
     4081 tgtgcggggg caggggcgcg gcgaccgcga ggccttcgaa attgagcgcg aaaatcaggc
     4141 ggcagttccg gcacccgccg cagggccgga gcgcgccgcc ggcggtccgc gtccgcgcct
     4201 cgcagttgag cagcgcggcg aacgagaccg ccagtcccca ccggccgcat ccgtccggcc
     4261 cgcagagaag gtaggtgccg gccacccggt cggactcgac cgaccgcgcc atcacttccc
     4321 aggcgcgggg ttgaaactgc gagatgtcaa aaggcatcga ggcgtatcca cagcaccgga
     4381 tcgaggggtt cgcgaccctg gcgcaactcg aactttacct ggccctccgg ccccacccgc
     4441 ccgagcacgg tggtccctgt caccgcgtcc ccggtgcgca cctggggctc gccgagcccg
     4501 gcgtaggtgg tgtagtagtc gctgtcatgg cttatgataa caaaccgccc gtatccccgc
     4561 aacgtcccga cataggcgac cgatccggcc gccaccgcgc ggaccgccgc cccgggcgcg
     4621 gccgcgatcg tgattcccgg cgagtacgac ttcagcttgg tgaccggatc ctgggccggc
     4681 ccgaacggca cggtcacttt ccccggcgcc ggcggtggga gctgcccctt cagggtggcg
     4741 aacgccgagg gaggcgcagg ctcgctcggc cggctttccc gccgctcccg ctgctcttcg
     4801 agcagcgcga tcacgcgctc gatttcatcg gccgcctgct gcagcgtaag catcttgtcc
     4861 ccctcggcga gcttggtccg gcggattttc tccagcgctt tctcccgctt ctctcgctgg
     4921 ctcaggtcga gcgccgtcgc cgtctctttg tcctttttga gcgactggac cttggtccgc
     4981 tggctagcga gcgtgtcgag catggcgagc gtctcgctca ggtaggcgcc ggcgtcggcg
     5041 acgttgtccg attcgaactg ggcgagggcg gcgaggtaaa cgatctggcg gttttcctcc
     5101 agccccttcg caggccccga ccagagcggc cgggccgtcc gcccggtggc cttcgcgtag
     5161 aactggcgga tatcgccgag atatttgcgg cgcgcccgtt cgagccggtc gcgcgactgc
     5221 ccgagacggt cctcggtcgc ctgcatctgc tcgtgcacgt cggccagttg gcgcgtcagc
     5281 cgcccgatca ctttcttgtt cgcggtgatc ttctggtcgt accgcgagat ctgttccgtc
     5341 gtctgcagct cggcgctgcg cagcgagtcg agcttcttct cgctccgctc gacgtccttg
     5401 cggatgcgct ccagctcttc ctgctggctg cggatgcggt ccttgtcctt gcccttgtcc
     5461 gcctgcacgt ccgccgcccc caggaccagc aggagggccg ccgcccacgc cgcacgtctc
     5521 acgcgagcat cctccgcacc ccgaggtacc cgctgaccac tccgaggagc gcggccccga
     5581 ggcagaagcc ggcgatttca gcaagcgggg ggaacaccgg cgcgacgtgg gtgaagctca
     5641 cctgtccgcg ggcgtaccag agcgccgccc agccgatggc cgccgccgct ccgccgagca
     5701 ccgccccctc gagcagcatc ggcgccacca ggaacagccg gctggctccg agcaggcgca
     5761 tctgcgccag tccctccgcc cgcgtgcgga tcatgagccg gagattgttc agcgagctga
     5821 tcacggccgt caccaagatc aggattccga gcgccaggcc gacccgcaga atgacggcct
     5881 gggtcccctc cgtcttgcgc agccactcgg cgttgtagta gaggtcggcc accgccggca
     5941 tccgccgcag ctctccggca atcgccgcga gaccgtcgct cgtgagcgcc gccggcgcga
     6001 acgtcagcac gaacgaccgc ggcagcgggt tgagggtgtc atacccggcg agcaggtcca
     6061 tgcccagcga cgcctgcatg atctctcgcg ccgcctcctt cgagatgtac tgaaggccgg
     6121 tcaccccggg gatcgcggcg acctccgcct ggacgcccgc gacctccccc tcggccactt
     6181 cctcccggac aaacacttcc atctgcacct cggccacggc ccgcgcgaac acctgctcga
     6241 ccgtcgccgc cccgatccag tacaggtcga acaggaggta cagcagggtc agggagagca
     6301 gcgatcccag cgccgtgaac gggtgccgcc cgaggctccg cgcaaattca cccgctatgt
     6361 gggccgtccg ggtcacgtca gcctcccctc ccggatcacg tagctggtgc tgttgggcag
     6421 ggtgccgggg ggatggtccg acatcaggag aaccatcgtc cggcccgaca gcgaggcttt
     6481 gaccaggtac tcgaacaccc ggcgcgccgt cgccgtgtcg agtcccgcca tcggctcgtc
     6541 gatcaccagc agcggctggt cggcgatcga ggcccgcgcc agttggacga gcgtttgctc
     6601 cacccgcgtg agcgtcgccg ggtaatcctg cgcgcgggtc aagagcgaga actcggtgag
     6661 catgcgcagg agccgttctt tgcgcgcccg cttgcgcacg cccgccagca ccagcgggta
     6721 agtgatgttg tcggccgccg tgtaggtcgg cacgaggccg aaaatgccgc cgacgccgcc
     6781 aagccgacgc cgcacccggt tgagcgcccg cccccgtccg cggcggagat tctcgccgaa
     6841 cacctcgacc gaccccgcgt ccgcaaactt ccgccccacg agcagttccg caatcgtggt
     6901 cttgcccgaa cccggccccc ccgtaatcac cgccgtctcc cccggtcgca ccgacaagct
     6961 cagattgcgg aaaagctctc cgctgcgggg ataggcgaga tcaacattgc tcagttcgac
     7021 gatcgcggtg tcgggcatac caactccatc cgttcgaacg accgttgacg ataacctcga
     7081 agttccgtat actgcggccg ttcgacgcca aaacttatac cggagtcgat ggtacaaaga
     7141 caaagaagcg gcctcaacta aaacaacgta tgagaaaata tctccttact ccccttgtgc
     7201 ttagcctgtg cctatcggcc ccgcgcgcgc aggactgggt ccccgtcacc ttgcccaccg
     7261 gcgacaacat caccggcatc gagttcctcc accccgacac cggctttctc tccattcagc
     7321 cgggcgacct cggccgcacc tatgacggcg gccgcacctg gacgatccac ccggtcgccc
     7381 tcgacctggc gctcgaagac gtctcgttcg cgaacgcgaa actcggcctg gtctgcggca
     7441 aagccggcgc cctgtaccgc acctccgatg gcggcgaacg ctgggagagc gtctccccgg
     7501 tcgggcggga cagcacggtc gcttttttca gcgtcaaatt catcgacccc cgcaccgcgt
     7561 tggtcaccgg catgagccgg cacgaggaga ccccctggag cggcatcgcc taccgctctc
     7621 tcgatggcgg ggcgacctgg accagactcg agcccatggg tctcatgtac agcgagattt
     7681 tctattcccc gaccgccggg ctggtctttc cggcgctcgg cctgctcaac cggtccgatg
     7741 acaaggggaa atcgtggcgt cagaacggcg cgatcgagag cgccctcaac cggagcgtcg
     7801 ccgtcgccgg cgacctgatc gtcctcgtcg gcccgaaagg tttcctcgcc gtttcccgcg
     7861 acaacggcaa gacgtggaag aaaatcgagc aggacgtcga acgcacgttt gtcgcggtca
     7921 ccgcgatcga cgaccgcacg gttttcgtcg ccggacccaa ctcgctcatg ctgcgctcgt
     7981 ccgacggcgg cgagacgtgg actcaggagc tgctggcccg ctcgttcgac gtccttgatc
     8041 tggcgcgggc gggcagccgt ctctacgctg tcggtaccga cggcgccgtg atctacaagg
     8101 aactcggccg caagtagggt agctgcgggc ccggccgccg ggccgcgccc gccatcgaat
     8161 ggcacccgaa ccgccccggc tcccggtccc ttacggacag gccgccggca ccgggccgcc
     8221 gcggaagaga tagccgatca ggagggtcag gtcggacaca gccggcgaac cgtccccgtt
     8281 cacatccatg tgggcggggc agcccgccga gactccgccc cggaacaggt acgcgatcgc
     8341 gaacgtcaga tccgacaccg ccaccgtccc gtcccccgtg cagtcgccgc gaagcataca
     8401 gcagcaggcg tctcccacgc cgtcgccgtc gaaatccgcc tggtccgggt tgggcacggc
     8461 cgggcagtta tcggcgtcgg cgcagaaaag atcctcgtcg gcgtcgttgt ccggatcgtt
     8521 cgggcaggcg tcgcagaggt cgccgagcgt atcgccatcg gcattagcct gctcggcatt
     8581 gggcagggcc gggcaattgt cgcaggcgtc ccctatcccg tcgccgtcgc cgtccgcctg
     8641 gccgggattg ccggcagccg ggcagttgtc gcacacgtcc ccgactccgt ccccgtcccc
     8701 gtcggcctga tcggcattgg cgactccggg gcaattgtcg gcgccgcagt actcggtcga
     8761 atagccggga tcggcgaaac cgtcgccatc cgagtcaatg caccagtcgc aggcgtcccc
     8821 gattccgtcc ccgtcgacat ccgtctgatc cgggttgctc agctcggggc agttgtcgtc
     8881 gggacactcg ttggccgcgt accccgggtt cccgtacccg tcgccgtcgc tgtctgtgca
     8941 cgcgtcgcag gcgtcccccg tcccgtcgcg gtcgagatca ccctgctccg ggttaggcag
     9001 accgaggcag ttgtcgcagg cgtcgccggc cccgtcgaca tcctcatcct cctgccccgg
     9061 gttatgcgcg agcgggcagt tgtcctcttg tgcgcacaac ccgtccccgt cgccgtcgtt
     9121 cagcgaatcc tccgggcaca cgtcgcagag atccccgctg ccgtcgccgt cggaatcagt
     9181 ctgtgcggga ttgtacgcgg ccgggcagtt gtccagttcg gcgcagtagc cgtcgccgtc
     9241 cccgtcatcg cacacccggc aggcgggctg tccggccccg atcaggacgc cgcaggtgtt
     9301 gaacgggctt tccggggcgc acggcgagag ttcgtcgata tcgagattcc ccgccgccgt
     9361 gtcgcagaag atcggcgggc gggtcatgtt gcccagccac gtgcggtacc gctcgatggc
     9421 cggcggccaa tccaccgtgt tgccgtagag gtcgctgcac tcgacccatt gccccccgta
     9481 gcctccgcac tcgtcggatg agaacgtcat accgccgtag gccgcgatgc agcggcgcac
     9541 atcgatgtac gatccgtgcc cgcctccggc aaaaccgcag ccgtagaatg tgcagccctc
     9601 catccgcaca ttgcgcccgt gatcgcacgc gaccccctgc tgcacgccga ggaagcggca
     9661 atccacgacc ttcacatagg caaactccgc cacgatcccg gcctggaggt cgccctggaa
     9721 gacgcagttg cgaatgnnnn nnnnnntgcc agcccggggc cgcccggaag ccgagtccct
     9781 ccactgtcgg cagcgtgtcg ctcgcctgga tcagtatggc cggcgggtag ttcgccagcg
     9841 ccacctgcac gattgtatgc tccggcccgt tcgctgaccg caccatcatc ggcatccggt
     9901 tccacaccat ccaattggtg ctgtctatcg tgtagatccc gtcttcgatc accaccgtat
     9961 cgcagtaatg ggccgccgcc agcgcctcgg cgatcgtcgg atactccgat gggacgtaca
    10021 gcgcagtcgg aagctgcgtc ggatcgcagc ggctcacctg gacgaggaag cagtagtcgg
    10081 cctgcccgcc ggacagcgcg tacagcgact cccactggtc gccgtcgtat acggtcgacc
    10141 ggtgctcccc cgccgacggg ccgtcgccgc tgtccgtcac cagaacgagc gtgtcgccgt
    10201 ctccgccgat cgcccccagg ccgatatgaa acgactggtc catctcgaag cccacatcca
    10261 gcgcccccag cggcaccgtt acccaggcga gtgttgtcgg gagcgaatcg tacggcacct
    10321 cggtcgaatc gagcagcgcg ccggggtatc cggagccgtt gtccgcgtac acgtagacgc
    10381 gcagtcccgg ctgcccggtc atgagcgaag gatagagcgc gagctgaacg gcgccgagcg
    10441 ccagttcgcc cggattgccg aaactggcga cgtcgaaccg cactgcggcc cgcgtggcgg
    10501 ccgaccggct cggcaagcgg tagatatacg cggccgatcc gcagtacgac acgtagtcgc
    10561 accccggaca ctcatagcgg ccgccgcccg cctgcgtgtc cgacagatcg gctgccggcc
    10621 gggagatcgg ctcaggacta ctcccggagg ggtctgcctg cagtcctccg gccgctccgg
    10681 caatggcgag ggccacggcc agcaaacagc ggacgaccgc tcttaataag cgagagcgca
    10741 taccattccc tgctcgtttt tggatcatgt ctcggatacc tatatctatg atacgacaat
    10801 cccccgtgtc gtcaagggca aactgcgact ggaattccct tgccctgttt gtcctcgttg
    10861 cgtattctga gtctgtcgca gaacaccatt tcgcggagga gcgaatgaaa cacgcactcg
    10921 ccattctcat cggccttctc ctctgcctcg cggtctcggc gccgctggcc caggaggacg
    10981 cgaaaccctg gttcgatctt gaaaaatgcg ctttctgcaa gcagcttctg tccgaggagg
    11041 gtctcatcga ccacatgcat cccgcgtacc acaacctcaa gtccggggtc ttgtggatca
    11101 cggttgtcga tccggagtac cgcgacgcgt tcaaacgcgc cggggcgaag atggaggaag
    11161 tcggcgccga gatgcagaag accggcgagg tcccctatat gtgcgggttc tgcagcgcgt
    11221 acggggagct gatgatggcc ggagcggttc cggaatgcgt cgaaaccgag gtcgccgaga
    11281 tctgcctcat gcagtccgac aagcccgagc tggtcgaaag gctccacgct ttcgccgccc
    11341 ggagcagcga agaaatggcc aagctgaagg cggcggaatc ggccgagtag cggccggtgc
    11401 caggcacagc cctttccaat cgggggtatc cggcgggtca ctcgcccgcc tcagcccact
    11461 gcccgcagtg attccggacc cgcccggcgg ccgccgttat ctgccggcct cgcggaaatg
    11521 cattcccgcg cccagcgcca gcgccacccc gatcaccacg tacacggcaa ccagcacgat
    11581 cggcggcacc cccgcgtggc gcagggcaat cccggctccg accatcgcgg ccacgatcac
    11641 gtacgactgc agcgactgga aggcaaagag gcagatctta tctttgtgag gggagagctc
    11701 gcggatacgg gcgacgttgc ggcgcgccac cgggcggagg gccaatcggt acttggcgac
    11761 ccccaggacc gcggcggcgc cccacaccca ggccgagccg cccggcagcc cccgggtgag
    11821 gatccctgcc cgccagagca gcacggccgc gccgatagcc caggcgacgc ccgccccgcg
    11881 gaggagactc gcccgcgaca ccgccggctc gggaatcgat acccgcacgc gccccatggc
    11941 tgcctttcac gaaaaaagca ggcgtccgca ccgcctgcct ttctctccct tcgcgcccgg
    12001 ccctcagtcc ttcatcgagg ccaggaattt ggcgttgttc ttggtcttct tcagccgccc
    12061 gaggaggaac tccatggcct cgatcgggtt catttccgcc aggaacttgc ggagaatcca
    12121 gatcttgttg agcgtatcct ggtcctgcag gagttcttcc ttgcgggtcg atgagcggtt
    12181 gatgtccatg gccgggaaaa tgcgccggtc ggagagccgg cgatcgagca ccatttccat
    12241 gttgccggtg cccttgaact cctcgaaaat gacttcgtcc atgcgcgacc cggtttcgat
    12301 cagggcggtg gcgatgatcg tcagcgaccc gccctcctcg atgttgcggg ccgccccgaa
    12361 gaaccgtttc ggcttgtgga gggcgttgga gtcgacgccc ccggagagga ttttgcccga
    12421 gtgcggcacg accgcgttgt gggcgcgggc gagccgggtg atcgagtcga gcaggatcac
    12481 cacgtccttg ccatactccg tcagccgctt ggccttctcc agcaccatgt gggccacctg
    12541 cacgtgccgc tccgccggct cgtcgaacgt cgaggacacc acctcgcccc gcaccgaccg
    12601 cttcatgtct gtgacctcct ccggccgctc gtcgatgagg agcacgatga ggatcacctc
    12661 gggatggttg gcggtgatcg cctgggcgat cttctgcagg atgatcgtct tgcccgcttt
    12721 cgggggcgag gtgatgagcg cccgctggcc cttcccgatc gggcacatga ggtcgataat
    12781 ccgggtcgtg acctcctccg caccgacctc gaggtggaac tgcacgttcg ggtagagggg
    12841 ggtgaggttg tcaaagaggg tcttgtgctt ggccacctcg ggatcgtcga aattgaccga
    12901 ctcaatcttc agcagggcga aataccgctc gttgtctttg ggcggccgga cctgccccga
    12961 gatcacgtcg ccggtccgca gatcgaaccg cttgatctgc gacggggaga cgtagatgtc
    13021 gtcctggccg ggcagatagt tgtaggacgg cgagcgcagg aatccgtacc cctcctcgag
    13081 aatctccagc accccttcgg ccagtatcat cccctcgccc gaggaagtcg actccatgat
    13141 cttgtagatc agttccgact tgcgcaatcc cgagacgccc gggatgtcga gttcctcggc
    13201 gattttgagg agatccgcga ttgtcttttg tttcagatcc gcaacttcca tttgaccacc
    13261 tgtccttatc ctgcctcttg ctcgcaatca gaattttcgt tttcacacgg cgtcggccag
    13321 ctcctccgca ggcgcgtctc cccagaggcg ctccagctgg tagaaccgcc gcgtcggttc
    13381 gtagaacaca tgtatcacga cgtcgacata atcgatgagc acccagttgc cggcctcagc
    13441 tccttcgacc tggtagggct tgatcccctg ttcgcgcaag ccgtcctcga tcgcccgggc
    13501 gatcgccttc acctgaacgt ccgcgtcgcc ggaggcgatc acgaagtagt cgcacaccga
    13561 cgagatctct ttgagcttca ggatttttac gtcaaagccc tttttggaga gcgccagata
    13621 accggccgcc agggccaact cgtgggagga taactcgctc aagactgctg ctgtgactcc
    13681 ttttttattt cctgtgcgga gagaaggtcc tcgatgtcgg ggccgagaat cagggtcgcg
    13741 gtgacctggc gggtgttgtg ctccaggggc tggaaccaca ccgcgtcgtc gtccagcccg
    13801 agctgttccg ccagcatccc ggccccctcc agatcctcgg tccgcgacac caccaccgtg
    13861 cgcgttacgg cccgcaaatc atacacgcct gtctcgacca cctggacctc gaggtcggcc
    13921 ccggccagcg ccttgacccg ctgcaccagc cggttcagcc cggccccgct gcccccggtc
    13981 tggaggacct gcaggcgcac gacctcggag ggcgggtttt ccgtcagcga caccccgcgc
    14041 acgagccggg tcccgaccaa ccccagatag atcgtcaccg ccgccaggcc ggccgtgagg
    14101 aaaatcgtcg tgaatttgcg aatcatgtgt ttgttcatgt cgaagcgggg tgccggcgcg
    14161 tcaggccgcc ggcgggatgg cgagagtcgg aaagcgcgga aatcgggaaa agctgcggta
    14221 gggggccgaa ttcactctcc tcgctttcag tagcggttcc accaggaccc gggcccgaac
    14281 aggctgcccc gccgatagta cggcccgtac caaccgccgg tatacgactg cattccgacc
    14341 gtcatccgaa aactctcgga cgggtgatag tcaagcataa accccggcag caccgtcgca
    14401 tcgctcacac cctctcccca cagcgccccg gcgttgtggg ccaccccgac attgagcagc
    14461 agcgacagct tcggcgaaaa ctcgtaaaac atactcgtcg tcagcatccc caccgaggcc
    14521 gattgcccgc cccccgacag gtaggacagc gagtagctgt gcgaccagtg caggcgggag
    14581 aggtcgatca gcgacagcgg gcttttcgcc ggttcgatcc ccatcacatt agcccgcgcg
    14641 gccgcttcca ggtcggctcg gtcggacatc tgggccgcgg ctgaagccgc cagagtcagc
    14701 gtggcgagga ttacgatcaa cttgtgcata tgcgctacca ccaattctaa caccggctta
    14761 atgtaacgcg ccgggaaggc cccggcaata aaaaaaaccc ggcccccttg acctagtatc
    14821 gacccggccc ccggtcatcc tttataacgt accggcccgg cgatccgctc actgatattt
    14881 cccgctcccg gcggccgggt agccgaattt tctggccagt tcggccaact gctcgaccga
    14941 gttgacccca agcacctcgt cggggttttc ggcagtcacc acggccaccc gcaagccctt
    15001 atcatgcatc accttaatcg tgtccgtcag atagtattcc ccctgtgcat tctcccgccc
    15061 gatctcggta agcgcggcga agaggtggcg gttgtcaaaa cacaaaatgc cggtgttgat
    15121 ctcccggatg cggcgcacct cgtcggaggc gtctttctcc tccacgattt ccgccagcat
    15181 gtcggtattt ccctcccgca cgatccggcc gtagccgtag gggttcgcga acaccgccga
    15241 cagacaggtg gccgccgccc cggtccggct gtgaacgtcc agcaatgctt ccagcgaccg
    15301 gcggctgaga aacgggacat cccccgccgc caccagcgtc gtcccggcga aatcgccgag
    15361 gagttctttc gtcatcatga cggcgtgccc ggtccccagc tgctcgcgct gccaggcgaa
    15421 ttcgaccggg cagccgcgca gcgcttcctg gaccatctcc cccttgtggc cgatcaccac
    15481 gacaatccgc tcgaaccgca tcggcagcag cgtatccagc aggatccgca ccatcgggcg
    15541 gccatcgatt tcatgcagca ccttcggcag gtccgacttc atgcgttttc ccttgccggc
    15601 cgccaggaca atcgctgcgc gtgtctcggg cattgaactc ctcaagtggg cgggtggatg
    15661 ccgtgcgggc cgggaggacc ggcccgtcgg tcacggcctg atctgtttgt tgttttggtc
    15721 gacatggacg accgccggtt tgtgcaccga cccgtccttg tggtcgatca gtccgtaggc
    15781 gatgataatt acgaggtcgc cgaccgcccc tttgtgcgcc gccgctccgt tgagggcgat
    15841 cgtcccgctc cccggcttcc ccttgattac gtatgtttcg aaccgttcgc cgttggtcac
    15901 gttggcgacc tggattttct cgtactccac gaggtcggcg gctttcatga gctgctcgtc
    15961 gatcgtcatc gagccgacat aatcgagcga gcactgggtg atcgtcgccc tatgtatttt
    16021 tgacttgcag accgttatca gcatttcgtc ttcactctcg tccggctgtt cggtccggcg
    16081 ccggggtaat atagccgccc ggcggcgtcc gtcaaggccc ggggttcctc gtttgtccgc
    16141 ttttcgaacc cccgagccgc cttaaagttc ccgcggccac ggcggcgcgg ccgcggaatc
    16201 cgccgtccgg cgccgcttac gcggtgatct gcacccagtt gcgggccggg aatatctcca
    16261 gctcgtcggt gatctcctca atcatccgct cgaggttctt ccaactgacg aggtgccgcg
    16321 ctttccccgg cggcagccag cggaaactgc tcacctcggg cgacagcttc acctgcgact
    16381 ccgggtcgac cacggcgaca atcagcggca ggatcagcac tttgtcgtag accggttcat
    16441 agaagtgcac gacgtactcg gtcgcccaca tgctcagggg attgagcccg agctcgacct
    16501 tgagcttgcg caccacgacc tgggccaccg tctccccgcc ctgcttgccg ccgttgacaa
    16561 acccccagta cccgccgtag gcatcctcgg ccgaccactg cagcaccagg aacttccatc
    16621 cgtccgggtc ctttttgacc acggcgacat tcacgcccgg cgattcgacc gggatcttag
    16681 tcgattcatt catggatatc cgctctgcct cacaaccatc tcattctctg caacttccgg
    16741 taatctacac cccccggcgc aaaggtcaag ccccttttcc gccggtccgc accgcgaaaa
    16801 cctcggctcc ggtctttccc cctcaacaaa tgccgccgtc cccccgtaat actcagtgtt
    16861 ttcggaacta ttcaaaaact gagagcagct tcttcaggag gagagcatga ccacgacccc
    16921 cggccgcaag gataaccgcc cggcatccgc cgccgtcgag acgctcattg gcgtctacgc
    16981 cgccgccccc gcccggctct ccggcgcact ggccggactg agcgaccatg agctcgcggc
    17041 acgccccatc gacgacaagt ggtcgatcca ggagatcgtt tttcacctcg ccgactccga
    17101 acttatggcc gccgcccggt tccgccaggc tatcagcggt tcgcatccca ctttcgcgta
    17161 ttacagccag gatatctggg cggagaagtt cgactaccgg catgccgacc ggcgtgcgct
    17221 ggaggaggcg gtcgacctgt tcgccgccct tcgggcggcc acgacccggc tgctgcggcg
    17281 cctttccggc gaccagtggg cgctcaccgg ccaccatccg gagcgcggcc cgctctcggt
    17341 ccggcagatt ctggagacgt acgccaacca cggcgagaac catgtgaagc agattctcga
    17401 acggcgccgg ctgctcggca agccgctgga cctcgccccg ctgacccctg gcgcctgagc
    17461 tgccggccgg gggcgtgcct gtcacgatct gttcccggcg agcggggcgg cccggcgctg
    17521 cgtgcgccac gccccccgcc ccctcacatc ctcatgttgt caatcagccg cacgccgtgc
    17581 acccgcaccg ccagcgagca gaccacgcct ggccgcaccg ccgccgcggg ctgcacgctc
    17641 cacaggtcgg tgaacgcgat gtagtcgatc tcggctgagg gacacgcacc gtggatcact
    17701 ttctccatct cggcccggat cttcttcgga tcgacggctc ccgctttcgc catcgcccgc
    17761 gccgtccgca gcgcgtagta gaggcagcgc gcctccgccc ggtgctgcgg ctccttgaag
    17821 taggcgttcc gcgaggacat cgccagcccg tcggactccc gcaccgtcgg cgcgatgacc
    17881 atccggatcg gatagtccag gtcccgggcg acctgccgca gcacgaccgc ctgctggtag
    17941 tctttcatgc cgaacacggc cacgtccggc cgcacgatgt tgaacagctt cgccaccacc
    18001 gtcgccactc cgaggaaatg ccccggccgc accgcacctt ccagcgcctc ggtgaccccg
    18061 ccgaccgtca cataggtctg gaaccccggc gggtacatgt cggcggcgga ggggataaac
    18121 acgagatccc cgccggccga ccgaatcttt ttcacatccc ccttctcatc gcgcgggtac
    18181 cgggcgaagt cctctcccgg ggcgaactgg gcggggttga cgaaaatcgt gaccaccacg
    18241 agatccgcag cctttttcgc ccgccgcacc agggccaggt gcccctcgtg gagatacccc
    18301 atggtcggga ccacgccgat tgtcttcccc gccgccgccg cctcccgcgc cgcccgctgc
    18361 atttgccgga tggaacgaat gattcgcatg ggcggtagat aacggtttgc cggtgcgaaa
    18421 tcaagcgggg ccgccgcctc cgaccgctgc cccgaggctg tccgcatcga ccggagcgcc
    18481 aattgaccca ccctcacccc gaggcaattc tgttacggtg acccggtgga aatcatcgcc
    18541 gatgccggcg aaacccgctg gttcatgcac acgcgcatgg gcggcgaggt ctcggcgttc
    18601 gaagtaccgg aggttgcctt ggactccctc atctttcgcg gtccttttgt gcccggctgc
    18661 ggtgccgggg cgccccccgc caccgccggg gccggcggcc gaccgccatg cgggccgccc
    18721 gtgtcattga cccctacgcc ttctgcctcg aactcgttgc tccaccctaa gcccttgtcc
    18781 cccaatatgg ctacggtggt cgtcgcccct tgccctcttt ttgtataatt cttgcacccc
    18841 ccggttgcat tatcggggcg gtacattata ttccatgact cggtttatcc cgctacggca
    18901 tgacccgata agactggata gaacggaact ggaaataagg agtttgaacg gatgttcgat
    18961 gcacttcgcc gcatgattct cccgattatc ctcatcgtcc tccttctctt cgcgggaatg
    19021 atcgttctcg agtggggcat ggggctttcg ggccggcaga ggtttgccga cgccaatgtc
    19081 gccggcgtcg tcaacggcga ggaaatctcg tgggagcgct acaacacgat cctcaacaac
    19141 atgatgcaga gcgagcagca ggactccgac gaggacctcc ctgacgccaa ggtccgcgag
    19201 ttgcagctcg aagcctggaa acaggtcgtc caggatcaga tcatgcagca gaaagtgcgc
    19261 gacgaggcga tcgtggtcac cgatgaggag ctgttctcct acctcgccta ctccccgccg
    19321 cccgagctgc ggacgctgcc gtacttcctg accaacggcc agttcgacta ccagaagtac
    19381 cagcaggcga tgctcgatcc ccaggccgcc cccttctggt cccagatcga ggccgcggcg
    19441 cgcaacgaca tcgccaagat gaaagttcag gagctggtgc tccaggacgt ccaggtgacc
    19501 gagaacgaag tccgcgagtg gttcctcggc tcgcaggaga aggtcacggt cggcatgatc
    19561 aacgtcgact tcgcccggtt ctcccgtccg gccccgacgg gcacggcgga ggagattgag
    19621 gcgtatttca acgcccaccg cgacaagtac acggtcgaag agcgcgccgc cctcaacgtc
    19681 gtcctcatcg agaagtcgcc cgcgccgtcc gactgggagg cctcctacga gagggccaga
    19741 gccatctacg actccatcaa agcgggcacc gacttcgccg agatggccgg cctctactcc
    19801 gaggatccgg gctcgaaaga gaaggggggc gacctcggct ggttcgcccg cggccagatg
    19861 gtcggcgagt tcgagaaagt ggcgttccag ttgaacaagg accaggtctc cgaaccggtc
    19921 aagacccagt tcggctggca catcatcaag ctctttgacc gcaagaccga gcgggaggtt
    19981 ccgcgcggca agaccgagcc ggaggatgtc gagaaagtcc tcgcctcgca catcctcatc
    20041 caggccaaac cctcgcagga gacgctcgat caggcctacc gccggctcga ggcgttccgc
    20101 acggacgcca agaagatcgg cttcttcaag gccgccgagg accaccagtt ccccgtgcgc
    20161 aacaccggcc ttttcttccg cgggcgcaac attcagtacc tcggcagcga tgcgcaggcc
    20221 ggcatgttcg ccttcgatga agccgtcgac gccatcagcg atgtctggga aaacaacagc
    20281 gccatttatg tcgtccaggt cgccgagaag cgtccggccg ggctggccac gttcgaggag
    20341 gcccgcgagc gcgtcactct cgacgtgcag caggagaaag tcatggcgct gtgcgtcgac
    20401 accgcggccg ccatctggaa cctgatccag caggggagcg acccgaagac cgccgccgag
    20461 cgcttcggcg agcagtacga aacccccgac cccttcaccc gctccggcta cgcgcccggc
    20521 atccgccgcg atccgctggc gatcggggcc gcattcggcc tgagtcggcc gggcgagttc
    20581 tccggaccgg tcaagtatga ccagggggtc gtgatcttcc ggctcctcag caaagaggct
    20641 cccgacatgt cggtgtacac ggagaagcgc gactccgtcc gcaccgttgt cctcaacaac
    20701 aagcggcagg agctgtttac gcggtggatg gaggagacgg tgaagagttc gaaagtggag
    20761 aactacgtcc agcgcgcgct ggtcgagcag cggggcatgc cctactgagt ttcgaccccg
    20821 gcgccgccga ttagcgaggc tcgcccgttt gcggcgagcc tctctttttg ccggtcccga
    20881 ccgccagccg cacaagcgcc gccgccgccc aggccccctc gaggaccacg aacggcagca
    20941 gaccgctcgc ccaggcatac cagcaggcga gcgccgcgcc cagcgcattc atcgccagat
    21001 aggctatccc gtgctccgac agccgcccca gcagactcag cacgaaggcg gccaggagga
    21061 gggccacgcc ggccgacccc ggcaccaacg ccgtcatgcg cgatccgtca tcccgcggcc
    21121 ctcagagatt ctcgtagttc ggcccctcgc ctccctgcgg cgtcatccag cggataatct
    21181 ggtagggatc ggcgatatcg caggtcttgc agtggacgca gtttgagggc gtgagctgga
    21241 gccgcacttt tcccggccgc tggtcgtcgt ccaccatctc gtacacctgc gcgggacaga
    21301 agtgctggca ggggttgccg tactcctcgg ggcaccggtt gttgcagatg tcgtagtcgg
    21361 cgatcaccag gtgcggcggc tgctcctcct cgtgcatcgt ccccgacttg tacacgtcgg
    21421 tgagtttgtc gaaggtgtag gcgttgtcgc acttcacgcg cgctttctcc ggccgccgcc
    21481 cgaaccggcg ttcgaatccg gcgagggtga gcatgtactc gtggtctggt tttttaacgc
    21541 ggcggtcgaa cagcccgcgc cccccggtca ccgtctgcac cgcggcgtgg aacattccgg
    21601 cgtagagccc gccctcgaag cccgcgtgga aattgcgcgt cttgcggagt tccgccccgg
    21661 cccagctccg ctcgaaccgc tccgcgtatg ccttgagccg cgccgtcgaa tagtccccgc
    21721 tcttcgcgca ttcgaagagc gtctcggccg cgagcatccc ccccttgatc gccaggtgaa
    21781 tccccttgag ccgggcggga tcgagcatgc cggcgccgtc gccgcacagg aggagaccgt
    21841 cgtggtacag cttgggcatc gcgaagcacc cgttgtcggg aatcgccttc gcgccgtagt
    21901 gctgcatctg gccgccgtcg agaaggcgcc ggatgaaggg gtgggttttg tagcgctgga
    21961 acttcatgtg ggggtcgttc gtcgggtcgg gactgttcag cccgaccgcg tacccgaccg
    22021 acaccatggt gtccgacatg gcgtagaccc agccgccacc gtactcatgg cggggctgcg
    22081 gccagccgat cgtgtggatc accgtccccg ccgcgatccg ccccgccggc acctcccaca
    22141 cctctttcac gccggtgagg tacgcctgcg gcacggcgcc ggcggtgagc gccgggatct
    22201 tctcgaacgc ctgccttgtc agcgaccccc gcactccctc gcacagcacc gtcaccttgg
    22261 cccggatgat cgaccccggc tcgaaattcg atttcggatt gccgtgcttg tcgaccccca
    22321 gatcgaccgt ctggagcccc gtcaccgcgc cgttctcgac cagcagttgg tacccggcga
    22381 gtccggcgta gatgtcgacg cccgcgccct cgacctgctc ggccagccag ccgacgaatt
    22441 tgttcaggga gatgatgtag ttgccgtggt tggacagggc gggcggctgg aaggggaatt
    22501 tcgtcgcccc gcgctcgctc agccagtaga gggagtccgc gcccaccttc gccgccagcg
    22561 gcgcgccgag cgtttcgaag tcggggatga gttcgcggat cccccgcgga tccatgaccg
    22621 ctcccgacag ggagtgggcg ccggcata
//
LOCUS       DAOPOE010000049        22502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2866_length_22502_cov_4.998797, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000049 DAOPOE010000000
VERSION     DAOPOE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_22502_cov_4.998797"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..651
                     /locus_tag="PKM94_07950"
     CDS             190..651
                     /locus_tag="PKM94_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66612.1"
                     /translation="MTKRPSIQTNEGAPDKHVSITATVVLRPDSTYPLVISPYKIDLS
                     QLGDKVIDQSPITFKNVSEQTLDIHLIAEEGDYFDLDLPKQIGPGQQVEGKVTLRASA
                     GTMSFDKSFTIEATPPGNGNGAAARFTVPVKRTVRVANQAGATKVLPRGGH"
     gene            complement(782..2242)
                     /locus_tag="PKM94_07955"
     CDS             complement(782..2242)
                     /locus_tag="PKM94_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOD66613.1"
                     /translation="MQPTRLIVLAAAALVAGAAPAQSGTGENLTVYAGFAVYPDPAYD
                     TVALVQVPFSLAQNEFEFFRPTEVDSFLYARIFAQIDLLGNAGVAVDSASTYFSVRVS
                     DSREAHETGIRIFNKLLLFARPGVYSARLTVIDAVSKRSGSAFLDRIVVEPPRRDAVA
                     LGGPYLAYSAAPFTDSSAVNLRLVENGLAVLPNPLGVFGTGDSTMYCVGEVYQPGKEV
                     GHAGPSTRLTFELLDSRDSLLRVLGARPALPGGATIAFTERFDIGGWLPGVYRLRVVA
                     DDSAAARADTQVVRFAVVSPADLLVMRDQSIPGDPYRLLTTDQRTALTWYFLSPEQQR
                     VLERLTPEGKETFLAQYWAEHDRDPATPLNEGRVDAVARFHYVNERFSLTEARDDGWR
                     TDRGRVYLVYGAPDEVDDRVVPLTDQPGIGADNSGRPYQIWYYRGVGEGKLFVFVDAR
                     SNNEYRLVHSNVYGEVYNKYWESLLQQGFPDLDRDL"
     gene            complement(2264..3607)
                     /locus_tag="PKM94_07960"
     CDS             complement(2264..3607)
                     /locus_tag="PKM94_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOD66614.1"
                     /translation="MRTFLRLTVLGLLVWVVAAAAAFGQSPLLRRAITGEAKKLIVGT
                     GVFPSEEPGRVRLEVYYQVYNYALDFQQLGGLFRARYVTTIRVNDRKADTTAAVSREE
                     REAVVDALAKTTSTADYRAAQVNFDLPPGDYEVLFLIENVGSSAILKRDFDLRLEPFD
                     SRSAELSDIELAQAAGPNESDSAAGAFAKGEFIVVPSLTGSFSGDDGGRLLFYLEIHR
                     GGDSLSEVTVVTALRHESKGMVYRDSLTTALPQRITRQLREISLDGFMPGDYRLEVSL
                     HGRRYKQLDQRQAEFRIPWTAASVLRYDWNAALDQLELIADGNEIKPLRKIDSLADRV
                     AALDRFWAERDPDPTTAVNEIKVVFYYRVDLANRLFSILNMEGWRTDRGRVLIRYGEP
                     DQIDDYPLVADRYPYQEWHYYKDRRYRKFVFVDVNDDGDYRLQYPYDGLYQRPDF"
     gene            complement(3715..4968)
                     /locus_tag="PKM94_07965"
     CDS             complement(3715..4968)
                     /locus_tag="PKM94_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66615.1"
                     /translation="MLMTFVEMRDCVLMALGSLRANKLRSGLTILGVLIGVASVIGLA
                     SLINGLNAAMEQEISGMGAAAIIEVERLPAMRDWDDLTEEERNRPELTVGEAEAIRDH
                     CPSVDGVAPTNHYWAPGGNVVKYQNRKVPNVRYFGTWPDYMRVRERSLQQGRFLGSMD
                     EARRAHVAVIGHDIANTLFEGRNPIGQEIRVNDERFEVIGVFEKAKSNFGNTYENNAV
                     AIPLSTFEKIHPWEEALGLSVRAVTLDKLEQAKEEVIGALRVYRKVPFGKENNFDLLT
                     QDAIREQVEKITDYIYIAMLVITSVGLMVGGIGVMNIMLVSVTERTREIGVRKAIGAK
                     RSNILLQFLTEATSLSGAGGTAGIIIGVLFGVAVNSAIGFPIAISVFWIVVGFLVAVS
                     VGMISGMYPAVKAAKLDPIEALRYE"
     gene            complement(4978..6198)
                     /locus_tag="PKM94_07970"
     CDS             complement(4978..6198)
                     /locus_tag="PKM94_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66616.1"
                     /translation="MVVLPILNLAVQALWAHKLRSGLTLLGVIIGVTSVITIVSALEG
                     MQDAIEAEFDSLGPTTFFVQRHGIIMSHQDWLEQMKRKRLEENLVPLLDESCDLCEVI
                     SPGVWTMSEVKFGDRSVRNVPVIGTTFNRVDIVDVEVQEGRWHSREDDLYKRRVAFLG
                     HVLYDELFNGQDPVGKDIRIGGRKYQVIGLAKASGSLFGDDNDKQVYIPFSAHRQDFD
                     SERDGIHLAIKARSLARLDDAMDQTRMILRAARHVPFDKPDDFAILTADSILDTLNNA
                     TRLFRMGLLGVSMISIVVGGIVVMNIMMVSVTERTREIGIRKAVGAKQTYILLQFLFE
                     ALLTTVFGGIIGIVLGFFIARALVGMINMDISPSALAVILGLGISTSVGLIFGLYPAM
                     KAARLDPIKALSYE"
     gene            complement(6203..6907)
                     /locus_tag="PKM94_07975"
     CDS             complement(6203..6907)
                     /locus_tag="PKM94_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66617.1"
                     /translation="MALIETRDLWRTYQMGAELVQALRGVSITIERGEYVAIMGPSGS
                     GKSTLMNLIGCLDTPTRGEYYLNGKAVSVMNDDELANIRNREIGFVFQTFNLLARATA
                     LHNVELPLIYNGSPAAERRRMAEEALARVDLADRMLHKPNELSGGQRQRVAIARALVN
                     RPSLLLADEPTGNLDSKTSIEIMRLFDQLHQQGNTIILVTHEPDIARHARRVLSIFDG
                     QIAGDERVPEHARVSY"
     gene            complement(6910..8229)
                     /locus_tag="PKM94_07980"
     CDS             complement(6910..8229)
                     /locus_tag="PKM94_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66618.1"
                     /translation="MSKKKLLIIIGVVVVVAVLVIANLTMNTSKAAKVQADVVTTREI
                     VERVSASGRVQPQTKVNITSEVNGEIIGLYVREGDRVEAGKLLVLLDTVQVASDVQQA
                     RYALSEIKARLDGAKTTLDQAEEEFRRQEKLMEKDLTSETIYKNARYAHLNAKATYEA
                     MLAQEQQAQSRLEKQLDYLQKCRITAPMPGVITFLDVEVGEIAAAQTAFTQGKTLMTI
                     SNLDVFEVEVEVDETEIVKVEQGQPVDIEIDAFPDTVFKGGVVEIGNTAILATTSSSG
                     MSTNFRVKVIFMDVNKKVRPGMSATVDIETARRDNALSVPYSAVVVRSLDRDSLEHSR
                     AGQGEGEPAAGSTSGVMAATAAESSAAGESGREAREEVRGVYVIRNGAAEFVAIQTGI
                     ADQKFIEVTSGLAEKDSVVAGPYRVLRTINPGDAVAPEAAVPMNEGE"
     gene            complement(8277..9680)
                     /locus_tag="PKM94_07985"
     CDS             complement(8277..9680)
                     /locus_tag="PKM94_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD66619.1"
                     /translation="MRFMRAIGMTVLAALAAAPGMVSAQQVLTLDDCIDLALKNRAAV
                     IAARGDYEIAKWDKVYAIGQFLPTVNAGYSYTDYRNWDQEAEQLVVTETDTVSWEGTL
                     MDDSPVTLRQEIPRATERRTVPIDDDEGTNKDLSISARLSLFNVPNWFDLAAAGAAKE
                     QARLNVLASEQDMVKSVKIAYYLYLAAVEGVAVDSEAVKRSEEQLKLIESKYELGSAS
                     RSDVLKQRVQMGNDRLTLLRSENSVVTTRAELAYVVGVDPRADVGFSTDYPRRSYEGG
                     LDEAIQYAMEHNPALLASGKSVDAARHSVKAAWATYLPTLTGSASMSANDGTRGDTVT
                     YDFSSKSRTFGLSLNWTIFDGFLRERQVAWAKVSLNNARAYRVDQLNSTVAQVKSFYL
                     EIKQVQEQLTVAGETVAAAEEDMRITQEKYNLGAATILDLLDAQVSLKEAQLGLIRAN
                     FDLNLAIARLESAMGKV"
     gene            complement(9693..10427)
                     /locus_tag="PKM94_07990"
     CDS             complement(9693..10427)
                     /locus_tag="PKM94_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOD66620.1"
                     /translation="MENVMAPAAPVEKVHHPGLGFKGLWQVFVAPSQLFAKVKEDPKV
                     LVPYIVLGLAILGLMLLLADYILQAQMQSEQFQKSLEQRGVTREQLTGQVLTFMKLST
                     VAAGTLGMLLVPLLIAALAAFWGKFVFAGRASFKALLSVSLFGEVLYALGSYVTIPLV
                     MAKKSILVSLSAAALLPNPDLTSLSYALLSKISLFHIWELIVLGIGFSIILGLPRNKG
                     IWVAVLSMGLLAILHALSSGIGAMFA"
     gene            complement(11159..12562)
                     /locus_tag="PKM94_07995"
     CDS             complement(11159..12562)
                     /locus_tag="PKM94_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOD66621.1"
                     /translation="MTRRSNRLLLLALAAAALTFYFGCSQPEDIVTPVTTSVLTLNPS
                     KLPSLPNGMVYELWAARSGDTVSLGRFGYDQTNRVFLNESGQVRPEGGSFRVGGDVLA
                     YDWIFMSVENAGAADTDLKAPANCLLRDRATNPRDNQVALVLPYSDSIFYSTCYFCLS
                     TPSDRDRDTNNMDAGLWFCQYQLEQVNRDVRIGLDSVTYQQKVYPQESIGVVIYTDYT
                     IDPENPLETLQLNYSFDTLEIIQARVTIDSVIISDPPETVMVANPYWQLGERIDTLFD
                     NFTQGTEGFGMVDYSEFGLQYRGWVMAQAVEAQNASVGTFTPPVWYRDNLFNVFRWPE
                     GSGLISTGAFGSVEDFDLGNPHSASTRVPPYPGEDFLVDLPHGAAGPLNLIAKGELRY
                     YPGDVFISLEPWNYKDTTTNFPLILYTGFLPFGAKGELYPDTTSDSPIQYFVMRNLTS
                     PDADIGMPKVIVDIDRF"
     gene            12782..14890
                     /locus_tag="PKM94_08000"
     CDS             12782..14890
                     /locus_tag="PKM94_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66622.1"
                     /translation="MRICPACKTEHISESATTCRECGHALEVPNADTAREDDLEFRVY
                     EKESDDREFVGGARDFDADADRPATPAPAEADAAGPPASTIDPDFSPIAGSEACVPEP
                     AEDDDASFGSQPPPEEPQLTASGKIRRLTEEEVKSIAGSLYRADAFLSDKDKKELMKK
                     LDDKEQLFAGSAPIEPPKRPEREKPKPADVLAEIENLGPSPKMAGRGRGVAYFYRNLI
                     QLRGSQQLFAGDEIAVAGKTYELRRKTIGRTPLLVGGGVAFVVILFLVATLFVSRPGR
                     EGRVAGIVLDDMQLPVNRGASVVFPELGQTIRVNGQGMFQTEALPPGSHKVEVLVNGK
                     TFASDYVTVAGGELSLIALKPMTGEEAAAAEPEAAAPSAVLGVTVPEPAPARETPGKT
                     AAPPKAKPKAQTSTGAAGSAGLKLAANVDGARLTVDGKVLGVGNLVYPQLPPGAHSYT
                     VSREGYAPATGTITLAEGKTQTLEVTLAPLSTAQKLEEYSAEDYYLSGLSAVQEQDYQ
                     AAVTDLRQAVGQEPGHADAWFALGQAHAGVKDWPNAHDAFLRAAEIYRFGNDHSRAIT
                     AYNAAIRVNDKSAAAYLGRGDLFLQRGDERAAAVDFETVVRLDRRNPHAYIGLGRSRM
                     NQGMYEEAIKHFKDARSIEPNNPVVYQYLMLCHMAESNFDEVRRSYAKFAETASENQM
                     RQLQADQRYAAALRVVNGHP"
     gene            14921..15217
                     /locus_tag="PKM94_08005"
     CDS             14921..15217
                     /locus_tag="PKM94_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66623.1"
                     /translation="MKERRHRLNCWEFKNCGREPGGLMAGLLGQCPVAAAMKYDGVNG
                     GLGGGRICWLVDRQEGCAGGGRCGTGRCHECDFYRRVVFEEDDAATYAFRTAPT"
     gene            15406..17349
                     /locus_tag="PKM94_08010"
     CDS             15406..17349
                     /locus_tag="PKM94_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD66624.1"
                     /translation="MRLLLVLATTAVLGSTLLLSCTAESESDGPVTPPVSVLNQGDYI
                     PDIATFLQIGGCSPTAYSWDGRDVYFTSSMSGASQVYRLTREGWPHQLTTFEDGIDFF
                     ALSYSGTMAVVGASVGGSEQSNLYLMDTRTGRVKQLTAGDQVQYGSVAWSRDDREIFF
                     RSNEENKRDFFIYRMDIATGEYRKIFGLPGYNGVADLSQDGSKMIVYQFNSNVDDDLY
                     LLDLASGEHRRITSDTSDVMYDSPTLMPDNKTIWLICNDNPDGLPRLARMTVDGPQVE
                     SVTDGWLDPKWAVEGLGFSRDYRFMAAILNEDGYARLKLREAATGKELKSPPLEGIVG
                     GGLFDRQGNCLISFNSPTRAPDVWRWNPQSEKLEQLTFSIYAGIDRELFREPQLVRYK
                     SFDSLEIPAFLYLPPNYKQGQPVPFIVNAHGGPEGQYRPYFERNLQYFLLNGFGLFAP
                     NVRGSDGYGRNYLNMDNYRNRKHSLMDYRAGVEWLIANGYAAPGMIGIRGASYGGYVV
                     LGMVTEFPDLFGAAVDEVGIANFQTFLENTAPYRRALRESEYGPLSDPAFLREISPIH
                     KAHLIKTPLLVVHGENDPRVPVGEARQIIKAVKQSGGVVDSLIFPDEGHGASKRVNII
                     AEYRKQVEFFTRHLKPAGEARKE"
     gene            17540..18289
                     /locus_tag="PKM94_08015"
     CDS             17540..18289
                     /locus_tag="PKM94_08015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66625.1"
                     /translation="MTAQEPWYEREDFWEWTAPFMFGEERFERAASSVGELTAFLRLA
                     SPAPVLDLCAGPGRFAVPLAAAGYSVTAVDRSRAFLATLTARARGRSLDIETVCCDMR
                     EFRREGAFEAVLNMYTSFGYFEDPEDDRRVLENVFSSLRPGGRLVMELHGRESLIRAF
                     RRRDWEEAHGITIIEERSFVDHFTRLHNRWIVLRGSERREFEFDLRIYGASELCALLR
                     SVGFASAEAYADFQGAPYDIAAGRLVALAAK"
     gene            complement(18282..19667)
                     /locus_tag="PKM94_08020"
     CDS             complement(18282..19667)
                     /locus_tag="PKM94_08020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD66626.1"
                     /translation="MPQLAAAARLRLQAGDAPGALECVRAVMRLAPHDPDALAVAGEV
                     AAAAGLEAEAEALLQRALAADPMLGSAHRGLCDLWGRVLARLAPDLRRKRDLLRTLHW
                     LSRYAPDCAAAPLLRENLRLREEALAAGRNRWVHADTRILLHLPPPGALRSLIENWAE
                     VLAYGGIPARLLRREDPTEPVFEQFRPDVFITLAEPAAQVGLDRDYIGRYRRAHGLRV
                     GHLTSFRHEYPPCDFLIALGCPADLPRADGAETPLLDMPPAINPLRHYMRPRAEVWDY
                     AFVGDNTPQTVERVKACLLPVLRLYSGVLAGRGWDIGLGELHQAEEAVLFNFARVCPN
                     LPLPGPSWESFAANLRALAIPACGGFALTDNTAFGGAFFAPDETAAADSPGRFVEMFD
                     YFRNHPDQRLPIIRAGMERVYREHTLFHRMDLLLAFLGCRGGSLRRERQPVPPWEESA
                     TRPVTWADRLT"
     gene            complement(19869..20282)
                     /locus_tag="PKM94_08025"
     CDS             complement(19869..20282)
                     /locus_tag="PKM94_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD66627.1"
                     /translation="MKHYREFLEFNTSRRREYINITASIEQALQRSAIREGLILVSAM
                     HITASVYINDAEPGLIQDIDAWLDKLAPHRDDYRYHRTGEDNADAHLKNLLLHHQVLL
                     PVSDGRIDLGPWQQVYYAEFDGRRRKRVIVKIIGE"
     gene            complement(20328..20900)
                     /locus_tag="PKM94_08030"
     CDS             complement(20328..20900)
                     /locus_tag="PKM94_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD66628.1"
                     /translation="MRSLGILCCLILAAACGLAGCSKDDDIVELEREVMAQQGQTDSH
                     PAAVSDVPVLPAAASAPDSVRPELNAGAIPDEPPDEPLNETPVEAQDVAPETTPDQPL
                     TPVDLPVPPAGDGFVVQLAGCEDPDYAAELVEKFRERGYEPFVSTETVGGETYHRVRL
                     GVYPTYAEARQVQQELSDKYSFDTWIDRIR"
     gene            21084..22076
                     /locus_tag="PKM94_08035"
     CDS             21084..22076
                     /locus_tag="PKM94_08035"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOD66629.1"
                     /translation="MRTVEHILDLVGHTPLVRLRTGMTDPGPRAYVKLEFFNPTGSVK
                     DRMAYYIITKALRDGRLKPGDTVIDNTSGNTGSAVAMVCSVLGLRAVLTTPVKTSQEK
                     IDLIKSFGAEVIVTRETEDHADPEGAYMVARRLAREHGYFDLDQYNSQENVEAHYRLT
                     GPEIWDDTEGRLTHFVAGIGTGGTFSGVARFLKEKNPAIKAIAVDPEGSVFAAYIRSG
                     RTTTPAAYKIEGIGSDVITEALHPQYIDEVITVSDRDAFQRARLIARTEGISGGGSSG
                     AVAVAIEQVARRARPEDIIVGIFADAGIRYLSKCYNDSWMHQHGFLPAGIPEKT"
     gene            22073..>22502
                     /locus_tag="PKM94_08040"
     CDS             22073..>22502
                     /locus_tag="PKM94_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD66630.1"
                     /translation="MTPKGRALISAVAFAAALPLAAWTGTQSPAPGKGLDFPTAVFDF
                     GHIGIDYTVRFRYPVINRSPDTVRVRKVDVHCDCSAAFVADSVLAPGDSTNIHLRFNT
                     RDFYGPQNKSLTITTDREGQSTIDLYYLSIIGQWFNGIKPD"
BASE COUNT         4079 a         7130 c         7417 g         3876 t
ORIGIN      
        1 tacgtgccgc agaactccac gatctcgcac tcattctggc tgttttcgac tggcgacgac
       61 acgctgaaga ttctgaaagt ggtgcctggg tgaggctgca cgaaggctcc gctggagaag
      121 tcggagcttg ctgtcggtga cagcacacgc ctgcaaatca ttttcgacac caagacgtat
      181 tcgagccggg tgaccaagcg cccgagcatt cagaccaacg agggcgcgcc cgacaagcac
      241 gtctcgatta cggccaccgt cgtgctgcgg ccggattcga cctatcccct ggtcatcagc
      301 ccctacaaga tcgacctgtc gcagctcggc gacaaggtga tcgaccagtc gccgatcacg
      361 ttcaagaacg tgtcggagca gaccctcgac attcacctga tcgccgaaga gggggattac
      421 ttcgatctcg atctgccgaa acagatcggg ccgggccagc aggtggaggg caaggtcacg
      481 ttgcgcgcct cggccgggac catgtcgttc gacaagtcgt tcacgatcga ggccaccccg
      541 ccgggcaacg gcaatggggc agcggcccgg ttcaccgtac cggtgaagcg gacggtccgc
      601 gtcgccaatc aggcgggagc cacgaaggtg ctgccgaggg gcgggcactg agcgcgcccc
      661 ggccggagcc ggaacggata aaactcgcac gccgccgatc ggatacgatc ggcggcgtta
      721 ttttgtcggc gggggcaggt cgaaccgggc cccgcccgga gcccgcaacc ctataccgct
      781 tctacaggtc ccggtcgaga tccgggaaac cctgctgcag caggctttcc cagtacttgt
      841 tgtacacctc gccgtaaacg ttggagtgga cgagccggta ctcgttgttg ctccgggcat
      901 ccacaaacac gaacagcttc ccctccccga cgccccggta gtaccagatc tggtacggac
      961 ggccggagtt gtccgccccg attccgggct gatcggtcag cggaaccacc cgatcatcca
     1021 cttcatccgg cgctccatac accaggtaaa cccgtccccg gtcggtacgc cagccgtcat
     1081 cgcgcgcctc ggtcagcgaa aaccgttcgt tgacatagtg gaagcgggcc acggcatcga
     1141 cccgcccttc gttcaggggg gtggcggggt cgcgatcgtg ctccgcccag tactgggcga
     1201 ggaaagtctc cttcccctcc ggcgtcaacc gctcgagcac tctctgctgc tcgggagaga
     1261 ggaagtacca ggtgagcgcc gtccgttggt cggtggtgag caggcggtag ggatcgccgg
     1321 ggatgctctg gtcgcgcatg acgaggagat cggccggcga gaccacggcg aaccgcacga
     1381 cctgggtatc ggcgcgggcg gcggcggagt cgtcggcaac cacccgcagg cggtacacgc
     1441 cgggcagcca gccgccgatg tcgaaacgct cggtgaaggc gattgtggcg ccgccgggca
     1501 acgccggccg ggcccccaac acgcgcagca gcgaatcccg ggaatcgagc agttcaaagg
     1561 tgagccgggt ggacgggccg gcatgcccga cctctttccc cggctggtac acctcgccca
     1621 cgcagtacat ggtgctgtcg ccggttccaa aaacgccgag cgggttgggc agcacagcga
     1681 gcccgttctc caccaggcgg aggttgaccg ccgatgagtc ggtgaaaggc gcggcgctgt
     1741 aggccaggta cggaccgccc aaggcgaccg cgtcccggcg cggcggctcg accacgatcc
     1801 gatccaggaa ggccgacccg gagcgcttgc tcaccgcgtc gattaccgtc agccgggccg
     1861 agtacacccc cggacgggcg aacaggagga gcttgttgaa gatccggatg cccgtctcgt
     1921 gggcttcgcg gctgtcgctc acgcgcaccg agaaataggt agaggcggaa tccacagcca
     1981 cgcccgcgtt tcccaggagg tcaatctgag cgaaaatacg ggcgtagaga aagctgtcga
     2041 cttcggtcgg ccggaaaaac tcgaattcgt tctgggccag cgagaacggc acctgaacca
     2101 gcgccaccgt gtcgtaggcc gggtccgggt atacggcaaa gccggcgtag accgtcaggt
     2161 tctcccccgt tccggactgc gccggcgcgg cgcccgccac gagcgccgcg gctgcaagaa
     2221 cgattaatcg ggtcggttgc atcagtgccc atccttcttc ggctcaaaaa tccggccgct
     2281 ggtagaggcc atcgtacgga tactgcaggc ggtagtcgcc gtcatcgttc acgtcgacaa
     2341 agacaaattt gcggtaccgg cggtccttgt agtagtgcca ctcctggtag gggtaacggt
     2401 cggcgacgag cgggtagtcg tcgatctggt ccggctcgcc gtagcggatc agcacccgcc
     2461 cccggtcggt gcgccagccc tccatgttga gaatgctgaa gaggcggttg gcgagatcga
     2521 cgcggtagta aaagacgacc ttgatctcgt tgacggcggt ggtgggatcg gggtcgcgct
     2581 ctgcccagaa gcggtcgagc gcggccaccc gatcggccag cgagtcgatc ttccgcaggg
     2641 gcttgatctc gttgccgtca gcgatcaact ccaactggtc gagcgcggcg ttccagtcgt
     2701 agcgcaggac gctcgcggcg gtccagggga tgcggaactc ggcctgccgc tggtcgagct
     2761 gcttgtaccg tcgcccgtgc agcgacacct ccagcctgta atcgccgggc atgaagccgt
     2821 ccagcgagat ctcccggagc tgtcgggtga tgcgctgcgg gagcgcggtc gtgaggctgt
     2881 cgcggtacac catccccttg ctctcgtggc gcagggcggt gacgacggtc acctcgctga
     2941 gggaatcgcc cccgcggtgg atttccaggt aaaagagcag gcggccgccg tcgtcgccgg
     3001 aaaaggagcc cgtgagcgac gggaccacga tgaactcgcc cttggcgaaa gcgccggcgg
     3061 ccgagtccga ttcgttcggc ccggcggcct gggccagttc aatatccgag agctcggcgg
     3121 agcggctgtc gaacggctcc aggcggaggt cgaaatcccg cttgaggatg gccgaggagc
     3181 cgacgttttc gatcaggaag agaacctcgt agtcgccggg cggcagatcg aaattgacct
     3241 gggcggcgcg gtagtcggcg gtcgacgtcg ttttcgccag cgcatcgaca accgcctcgc
     3301 gctcctcgcg cgacaccgcc gccgtcgtgt ccgccttccg gtcgttgacc cgaatcgtcg
     3361 tcacgtagcg ggcgcggaac agcccgccca actgctgaaa gtcgagggcg tagttgtata
     3421 cctggtagta gacctccagg cgcacccgac ccggctcctc cgacggaaag acgccggtgc
     3481 cgacgatcag cttcttggct tccccggtga tggcgcggcg caggagcggg gactgcccga
     3541 aagcggcggc cgcggcgact acccacacca gaagtccgag gacggtcagg cgaaggaagg
     3601 ttctcaccat atcacccttt aaacgaaggg gttttccggt ccgttccaag gtatcgacgg
     3661 cggtaccccg gcggcaagat caaaaatatc cggggccggg aagaaagaaa ccagttattc
     3721 gtagcgcagg gcctcgatcg ggtcgagctt ggccgccttg actgccgggt acatgccgga
     3781 aatcatcccc actgagacag cgaccaggaa gccaacgacg atccagaaga cggagatggc
     3841 gatggggaag ccgatcgcgc tgttgacggc gactccgaag agcacgccga tgataatgcc
     3901 ggccgttccg cccgcgccgc tgagggaggt ggcctcggtg aggaactgca ggaggatgtt
     3961 ggaccgtttg gcgccgatcg ccttgcggac cccgatctcc cgggtgcgtt cggtgaccga
     4021 gacgagcatg atgttcatca ccccgatccc ccccaccatc agtccgaccg aggtgatcac
     4081 gagcatggcg atgtagatgt agtcggtgat cttctccacc tgctcgcgga tggcgtcctg
     4141 ggtcagcagg tcgaagttgt tctcttttcc gaaggggacc ttgcggtaga cgcggagggc
     4201 gccgatgacc tcttccttgg cctgctcgag cttgtcgagg gtgacggcgc gcaccgacag
     4261 cccgagcgcc tcctcccagg ggtggatctt ttcgaaggtg gacagcggga tggcgacggc
     4321 gttgttttcg taggtgttgc cgaaattcga cttggccttt tcgaagacgc cgatcacttc
     4381 gaaccgttcg tcgttgaccc ggatttcctg gccgatcgga ttgcgtccct cgaagagggt
     4441 gttggcgatg tcatggccga tgacggccac gtgggcccgc cgggcctcgt ccatgctgcc
     4501 caggaaacgc ccctgctgga gcgagcgctc ccgcacccgc atgtagtccg gccaggtgcc
     4561 gaaataccgc acgttgggga ctttccggtt ctggtatttg acgacgttgc cgccgggggc
     4621 ccagtagtga ttggtcggcg ccacgccgtc gaccgacggg cagtggtcgc ggatcgcctc
     4681 cgcctctccg accgtcaact ccggccggtt gcgctcctcc tcggtcaggt cgtcccagtc
     4741 gcgcatggcg gggaggcgtt cgacctcgat gatggcggcc gcgcccatgc cggagatctc
     4801 ctgttccatg gcggcgttga ggccgttgat gagcgaggcc aggccgatga ccgaggcgac
     4861 gccgatcagc accccgagga tggtcagccc ggagcgcagt ttgttggcgc gcagagagcc
     4921 gagcgccatg agcacgcagt ccctcatttc gacaaaggtc atcagcatgg gccgtcctca
     4981 ctcatagctc agcgccttga tggggtcgag ccgggcggcc ttcatcgccg ggtacagacc
     5041 gaagatgagc ccaaccgagg tggaaatgcc caggccgaga atgaccgcca gcgccgacgg
     5101 ggagatgtcc atgttgatca tcccgacgag ggcccgggcg atgaagaagc cgaggacgat
     5161 gccgatgatg ccgccgaaga cggtcgtcag cagggcttcg aacaggaact gcaggaggat
     5221 gtacgtctgc ttggccccca ccgccttgcg gatgccgatc tcgcgcgtcc gctcggtcac
     5281 cgagaccatc atgatgttca tgacgacgat cccgcccacg acgatcgaaa tcatcgatac
     5341 tcccaacagc cccatccgga acaggcgggt ggcgttgttg agcgtgtcca ggatgctgtc
     5401 ggccgtgagg atggcgaaat catcgggctt gtcgaagggc acgtggcggg cggcccgcag
     5461 gatcatgcga gtctggtcca tggcgtcgtc aagccgcgcc aggctgcgcg ctttgatcgc
     5521 caggtggatg ccgtcgcgct cgctgtcgaa gtcctgacgg tgggcgctga acgggatgta
     5581 aacctgcttg tcgttgtcgt cgccgaagag actcccggac gctttggcca gcccgatgac
     5641 ctggtacttt cgtccgccga tacggatgtc cttgccgacc gggtcctggc cgttgaagag
     5701 ttcgtcatag agcacgtggc cgaggaaggc cacccgccgt ttgtagagat cgtcctcccg
     5761 cgagtgccac cgcccctcct gcacctcgac gtcgacgata tcgacgcggt tgaaggtggt
     5821 gccaatgacg gggacgttgc ggacgctgcg gtcgccgaat ttcacctcag acatcgtcca
     5881 tacccccggc gaaatgacct cgcacaggtc gcagctctcg tccaaaagcg ggacgaggtt
     5941 ctcttcgagc cgcttgcgct tcatttgctc gagccagtcc tggtgggaca tgatgatgcc
     6001 gtggcgctgc acgaaaaagg tcgtcgggcc gaggctgtcg aactcggcct cgatggcgtc
     6061 ctgcataccc tcgagggcgg agacaatcgt gatcacgctg gtaacgccga tgatcacgcc
     6121 caggagcgtc agcccggagc ggagcttgtg ggcccagagg gcctgcacgg cgaggtttag
     6181 gatggggagg acaaccatac catcaatagc tcacgcgggc atgttccggc acccgctcgt
     6241 cgccggctat ctgcccgtca aaaatcgaca gcacccgccg ggcgtgccgg gcgatgtccg
     6301 gttcatgggt gacgaggatg atcgtgtttc cctgctggtg gagctggtcg aagaggcgca
     6361 tgatctcgat ggaggtcttg ctgtcgaggt tgccggtcgg ttcgtcggcc agcaagagcg
     6421 acggccggtt gaccagcgcg cgggcgatcg ccacccgctg gcgctggccg cccgagagct
     6481 cgttgggttt gtggagcatg cggtcggcca ggtcgaccct cgcgagcgcc tcctcagcca
     6541 tgcgccggcg ctcggcggcc ggggaaccgt tgtagatgag gggcagctcg acgttgtgca
     6601 gggccgtggc ccgggccagc aggttgaacg tctggaacac aaaaccgatt tcccggttgc
     6661 ggatgttcgc cagctcatcg tcgttcatca ccgacacagc tttcccgttg aggtagtatt
     6721 ccccccgggt gggcgtgtcg agacagccga tgaggttcat gagggtcgac ttgccggaac
     6781 cggaaggccc catgatggcc acgtactcgc cgcgttcgat ggtaatggag acgccgcgca
     6841 gcgcctgcac gagttcggcc cccatctgat acgtgcgcca gagatccctg gtctcaatga
     6901 gcgccatggc tattcgccct cattcatcgg gactgccgcc tccggcgcga ccgcatcgcc
     6961 cggattgatg gtgcgaagca cgcggtacgg tccggccacg acagagtctt tttcggccag
     7021 cccggacgtg acttcgatga atttctggtc ggcgatgccg gtctggatgg cgacgaattc
     7081 ggccgcgccg ttgcggatga catagacgcc gcggacctcc tcgcgcgcct cgcgccccga
     7141 ttccccggcg gccgaggatt cggcggccgt cgccgccatc acgccgctgg tcgacccggc
     7201 ggccggctcc ccctcaccct gacccgcccg gctgtgctcc agcgagtccc ggtccagcga
     7261 gcggacgaca actgcgctgt aaggcacgga gagggcgttg tcccgacggg ccgtctcgat
     7321 gtcgacggtc gccgacatgc ccggccggac cttcttgttc acgtccatga aaatgacttt
     7381 gacccggaaa ttggtcgaca tgcccgagga cgacgtggtg gcgagaatgg cggtgttgcc
     7441 gatctccacg actcccccct tgaacaccgt gtccggaaag gcgtcgatct cgatatcaac
     7501 cggctggccc tgttcaacct tgactatctc tgtctcgtcg acctccacct ccacctcgaa
     7561 cacgtcgagg ttggaaatgg tcatgagtgt cttgccctgc gtaaacgccg tctgggcggc
     7621 cgcgatctcc cccacctcga catcgaggaa ggtgattacc ccgggcatgg gagcggtgat
     7681 ccggcatttc tggaggtaat cgagctgctt ttcgaggcgc gactgggcct gctgttcctg
     7741 ggccagcatc gcctcatagg tcgccttggc attcaggtgg gcgtagcggg cgttcttgta
     7801 gatggtctcg gaggtgagat ccttctccat cagcttttcc tggcggcgga attcctcctc
     7861 cgcctggtcg agcgtggtct tggccccgtc caggcgggcc ttgatttcgc tcagcgcgta
     7921 gcgggcctgc tgcacatcgc tggccacctg cacggtatcg agcaggacga gcagtttgcc
     7981 ggcctcgact cggtcgccct cgcgcacata gagtccgata atttccccat tgacctcgga
     8041 ggtgatgttg accttggttt gcggctgcac acgtcccgag gccgacaccc gttccacgat
     8101 ctcgcgcgtc gtgacgacgt cggcctgcac cttcgccgct ttcgacgtgt tcatggtcag
     8161 gttggcgatc accaggaccg cgacgacaac cacgacgccg atgatgatca gcagtttctt
     8221 cttcgacata gacgactcac ttatcctatc gtatacgcct gaagccggcg gccggctcag
     8281 accttcccca tggcgctttc gaggcgggcg atggcgagat tgaggtcgaa gttcgcccgg
     8341 atgagcccca actgagcctc cttcagggac acctgggcgt ccagcaggtc gagaatcgtg
     8401 gccgccccca ggttgtattt ctcctgggtg atgcgcatat cctcctccgc ggcggcgacc
     8461 gtttcgccgg ccacggtgag ctgctcctgg acctgcttta tctcaaggta gaaactcttg
     8521 acctgggcga ccgtgctgtt caactggtcg acccggtagg cgcgggcatt gttcagcgac
     8581 accttggccc aggcgacctg ccgctcgcgg aggaacccgt cgaaaatcgt ccagttcagc
     8641 gagagaccga aagtgcggct cttcgacgaa aaatcgtagg tgaccgtatc cccgcgcgtg
     8701 ccgtcgttcg ccgacatcga ggccgagccg gtcagggtgg gcaggtaggt cgcccaggcc
     8761 gctttcaccg aatgacgggc ggcatccacc gatttcccgg aggccaacag cgcggggttg
     8821 tgctccatcg catactggat cgcctcgtcg agcccccctt catagctgcg ccgcggatag
     8881 tcggtggaga atcccacgtc ggcgcgcggg tcgacgccga cgacgtaggc cagctccgcc
     8941 cgcgtggtga ccacagagtt ctccgaccgg agcagagtga ggcggtcgtt ccccatctgg
     9001 acccgctgct tgagcacgtc ggagcgcgag gccgaaccga gttcgtactt ggactcgatc
     9061 agcttcaact gctcctcgct gcgcttcacc gcctccgaat cgaccgcgac cccctcgacg
     9121 gcggccaagt agaggtagta ggcaatcttg accgatttca ccatgtcctg ctcggaggcg
     9181 agcacgttga gacgggcctg ctctttggcg gcgcccgccg ccgccaggtc aaaccagttg
     9241 ggcacattga acagcgagag gcgggccgaa atcgagaggt ccttgttcgt gccctcgtcg
     9301 tcatcgatcg gaacggtacg ccgttccgtg gcgcgcggaa tctcctgcct cagggtgacc
     9361 ggagaatcgt ccatgagcgt cccctcccag ctcaccgtgt cggtctcggt caccaccaac
     9421 tgctcggcct cctggtccca attgcggtag tcggtatagg agtagccggc gttgacggta
     9481 ggcaggaact ggccgatcgc gtagaccttg tcccacttgg ctatctcata atcgccgcgg
     9541 gcggcgatca cggcggcccg gtttttcagg gccaggtcga tgcagtcgtc cagcgttagc
     9601 acctgctgcg cggaaaccat ccccggggcg gccgcaagcg cggccaaaac agtcattcca
     9661 atggcacgca tgaacctcat tcactgtctc ctctatgcga acattgctcc gattccgctt
     9721 gagagcgcat ggagaatcgc cagcagtccc atcgataaga ccgccaccca gattcccttg
     9781 ttgcgcggaa ggccgagaat gatcgaaaaa cctatcccca gaacgatcag ttcccagatg
     9841 tgaaacaggc tgatctttga caacagagcg tagctcagcg aggtcaggtc ggggttgggc
     9901 aggagggcgg ccgccgagag cgacaccagg atgctcttct tcgccatcac cagcgggatg
     9961 gtcacgtaac tgcccagcgc atagagcacc tcaccgaaca gcgacaccga caggagggcc
    10021 ttgaacgatg cgcgaccggc gaagacgaac ttgccccaga aggccgcgag cgcggcaatg
    10081 agcagaggca ccagcaacat cccgagcgtg ccggcggcca ccgtcgacaa cttcataaac
    10141 gtgagcacct gcccggtcaa ctgctcgcgg gtgacgccgc gctgttcgag cgatttctgg
    10201 aactgctcgc tctgcatctg ggcctggagg atgtagtccg ccaacagcag catcagcccc
    10261 aggatggcca ggccgaggac aatgtacggc acaagcacct tgggatcctc tttgaccttc
    10321 gcgaacaatt gagagggagc cacaaagacc tgccacagcc ctttaaaccc cagcccgggg
    10381 tgatgcactt tctccacggg cgccgcggga gccatcacgt tctccatctt cagtttctcc
    10441 tcatggaaca gttgtacgca tccgtccggc cagtgtttca ctctccccag ccgtcggctg
    10501 caattggtac ccccgattcg gcggcttatt caacgcggca cgtcaaaatt gaccgggagc
    10561 cgtcccacag gatagctcca caatacgacc ggatcaagct agattcggat taatggacga
    10621 cgactgcgcc accgcgcctg cccgcggtgt gatcggccgg cgcatgagcg ggcatgtcgt
    10681 cggtcgccct cagtcggcgt ttcagaaaac catggcgact gcagcggccg cgcaccggac
    10741 aggcggcggt accgctgcgg cggaggagaa tcccggttgc cgcgcgcgat caaaggagct
    10801 gatggctgga ggtcctcctc aatgtcgtcg ctgcattcga cgcctggtct ggacccggct
    10861 tccgcgttcg aggcgggcag agcacccggc gccgctgccg gtggcgcgat aatgcggcag
    10921 atcatgagcc gcggtgggag cgggcgccag gggggccctg tgagccaaag catcgtgtcg
    10981 gctgtaagac cttgccctgc tgtaacttga ccccgccggg caacgccgtc cagaatcact
    11041 tgagtaggcg gtggcgagac cactccgaga tgtgccggac tggcgccggg cggccggaaa
    11101 aagcaaagag gccggcgcgc gccggcctct gtcaagtctt gctcggagaa gcgatcggct
    11161 agaatcggtc gatatccacg atcactttgg gcatcccgat gtccgcgtcc ggcgaggtca
    11221 ggttccgcat gacaaagtac tggatggggc tgtcgctggt ggtgtcggga tacagctcac
    11281 ccttggcgcc gaacggcagg aacccggtgt agaggatgag cgggaaattg gtggtagtat
    11341 ccttgtagtt ccatggctcc agcgagatga acacatcccc aggatagtac cgcagttccc
    11401 ctttggcgat gagattcagc gggcccgccg cgccgtgggg gagatccacc agaaaatcct
    11461 cgcccgggta tggcgggacg cgcgtactgg ccgagtgcgg gtttcccaaa tcgaagtctt
    11521 cgaccgaacc gaaagcgccg gtgctgatca gaccgctccc ctcgggccag cggaagacat
    11581 tgaacaggtt gtcgcggtac cagaccggcg gcgtaaacgt acccacgctc gcgttctgtg
    11641 cctccacagc ctgcgccata acccaccccc gatactgcag gccgaactcg ctgtagtcga
    11701 ccatcccgaa tccctcggtg ccctgggtaa agttgtcgaa gagggtgtcg atgcgctccc
    11761 ccagctgcca atagggattg gcgaccatga ccgtctcggg cggatcggag atgatcacgc
    11821 tgtcgatcgt gacccgcgcc tgaatgatct caagggtgtc gaaggagtag ttcaactgca
    11881 gcgtctccag cgggttctcc gggtcgatcg tgtagtcggt gtaaatgacg accccaatag
    11941 actcctgcgg atacaccttc tgctggtagg tcacgctgtc cagcccgatc cgcacgtcgc
    12001 ggttcacctg ctcgagctga tattggcaga accataggcc ggcgtccatg ttgttcgtgt
    12061 cgcggtcccg gtcggagggg gtggacaggc agaagtagca ggtagagtaa aagatcgagt
    12121 cgctgtaggg gagcacgagc gccacctggt tgtcgcgcgg attggtagcc cgatcgcgca
    12181 gcaggcaatt ggccggcgcc ttaagatcgg tgtccgcggc tccggcattc tcgaccgaca
    12241 taaagatcca atcgtaggcc agaacatcgc cgcccacccg gaaggacccg ccctcggggc
    12301 ggacctgtcc gctctcgttt aggaaaacgc ggttggtctg gtcatacccg aaccggccga
    12361 gagagacggt gtcgccggac cgggccgccc acagctcata gaccatgccg ttcggcaggc
    12421 tcggcagttt gctcgggttc agggtgagta cgctggtcgt caccggcgtt acaatgtcct
    12481 cgggctggct gcagccgaag tagaaagtga gcgcggcggc tgctaaggcc agcagcaaca
    12541 gccggttgga gcgccttgtc atgtactgta tcctcctcct aggtgacagg agctatcatt
    12601 cgttatcgca actaattggg gagtataacc cccccgccca aggggtgtca agtcattctt
    12661 agcgaaaggt ttcatctttc ttttcagacg cggagcggcc cccggccatc taaaggatta
    12721 tttccgctaa gtcgataaat aggtagaaaa tcgagaagtc cctaaagcac tggagcaaat
    12781 cttgcgaatc tgccccgcct gtaaaaccga acacatatcg gagtcggcca ccacgtgccg
    12841 cgagtgcggg cacgctctgg aggttccgaa cgccgatacg gcccgggagg acgatctcga
    12901 attccgggtg tacgagaaag agtccgacga ccgcgagttt gtcggaggcg cccgcgattt
    12961 tgacgccgat gccgaccgcc ccgccacccc ggcgccggct gaggccgacg cagccgggcc
    13021 gccggcctcg acgatcgacc ccgacttctc cccgatcgcc ggctccgagg cctgtgtgcc
    13081 ggaaccggcg gaagacgacg atgcctcgtt cggttcacag ccgccgcccg aagaaccgca
    13141 gttgacggct tccggcaaga tccggcggtt gaccgaagaa gaagtgaaat cgatcgccgg
    13201 cagtctctac cgcgccgacg cgttcctcag cgacaaggac aagaaggaac tgatgaagaa
    13261 gctggatgac aaggagcagt tgtttgcggg cagtgcgccg atcgagcccc cgaaacgacc
    13321 ggagcgggag aagccgaaac cggccgatgt gctggcggag attgaaaacc tcgggccctc
    13381 gccgaaaatg gccggccggg gccgaggggt ggcctatttc taccgcaacc tgattcaact
    13441 gcggggcagc cagcagctct ttgcgggtga cgaaatcgcc gtggccggaa aaacctacga
    13501 actgcgccgc aagacgatcg ggcgcacccc cctgctggtc ggtggcggcg tagccttcgt
    13561 cgtcatcctg ttcctcgtcg ccacgctctt cgtgtcccgt ccgggccgcg aggggcgagt
    13621 cgccgggatc gtcctggacg acatgcagct gccggtcaac cggggcgcgt cggtcgtctt
    13681 tccggagctc ggccagacta tccgcgtcaa cggccagggg atgttccaga ccgaggctct
    13741 gcctccgggc agccacaagg tcgaggtcct cgtcaacggg aagactttcg cttcggacta
    13801 cgtgacggtc gcgggaggag aactgagcct gatcgcgctc aagccgatga ccggcgaaga
    13861 agcagcggcg gcggagcccg aggcggcggc tccatcggcc gtcctgggcg tgactgtccc
    13921 tgagccggcg ccggctcggg agacgccggg caagaccgcg gctccgccga aagcgaagcc
    13981 gaaagcccag acctcgaccg gcgcggccgg ttcggcggga ctgaaactcg cggcgaatgt
    14041 cgacggcgcc cggctcacgg tcgacggcaa ggtcctcggg gtcggcaacc tcgtctaccc
    14101 gcagttgccg ccgggcgcgc actcgtacac ggtcagccgg gaggggtacg ctccggcgac
    14161 cgggaccatc acgctggccg agggcaagac gcaaaccctg gaggtgacgc tggcgccgct
    14221 atcgacggca cagaaactgg aagaatattc tgcggaggac tactatctct ccgggttgtc
    14281 ggccgtgcag gagcaggatt accaggcggc cgtgaccgat ttgcgccagg cggtcggcca
    14341 ggagcccggg cacgccgatg cctggttcgc gcttggccag gcgcacgcgg gcgtgaaaga
    14401 ctggccgaat gcccacgatg cctttctccg ggccgcggag atttaccgtt tcggcaatga
    14461 ccacagccgg gcgatcacgg cgtacaatgc ggccatccgg gtgaacgaca agtcggcggc
    14521 cgcgtacctc gggcgcggcg acctcttcct ccagcgcggc gacgaacggg cggcggcggt
    14581 ggatttcgag accgtcgttc ggctcgaccg gcgcaatccg cacgcctaca tcgggctggg
    14641 acggtcgcgc atgaaccagg gcatgtacga ggaggcaatc aagcacttca aagacgcccg
    14701 ctcgattgaa ccgaataacc ccgttgtcta tcagtacctc atgctctgcc acatggccga
    14761 gagcaatttt gatgaagtcc ggcggagcta cgccaagttc gccgagaccg ccagcgagaa
    14821 ccagatgcgc cagttgcagg cggaccagcg ctacgccgcc gcgctgcggg ttgtcaacgg
    14881 acatccatga ggagccaccg cttagccgga atctgagaac atgaaggaac gacgtcaccg
    14941 cctcaactgc tgggagttca agaactgcgg ccgcgagccg gggggactca tggcgggcct
    15001 cctgggccag tgccccgtcg ccgcggcaat gaagtacgac ggcgtcaacg gcgggctcgg
    15061 cggcgggcgc atctgctggc tggtcgaccg ccaagaggga tgcgcgggcg ggggacggtg
    15121 cgggacggga cgctgccacg aatgtgattt ctaccgccgc gtcgtgttcg aggaagacga
    15181 cgccgcgacc tacgccttcc gcacggcgcc cacataggcg ctccaacttg ccgcccacca
    15241 gcatacgcgg gccgggaacg gacgttcccg gcccgcgctt gtttgtcccc ggtctgcact
    15301 ccccgccctc cccgcgtttt tgggttgcca cgtgaacatt ccgtttctat tttcctctca
    15361 cctggtagaa cgacccgtac cgcgaactct agtcaggaga accgcatgcg acttctgctc
    15421 gtacttgcga ccacggcggt gctcggcagc acgcttcttc tatcctgcac ggccgaatcg
    15481 gagtcggacg gtcccgtcac accgccggtg tccgtcctca accaaggcga ctacatcccc
    15541 gatatcgcca ccttcctcca gatcgggggc tgcagcccga cggcgtacag ctgggacggc
    15601 cgcgacgtct acttcacgtc cagcatgtcg ggcgccagcc aggtgtaccg gctcacccgg
    15661 gaaggctggc cgcaccagct cacgacgttt gaagacggca tcgacttctt cgccctgtca
    15721 tactccggga cgatggcggt ggtgggggcc tcggtcggag gctcggagca gtcgaacctc
    15781 tacctgatgg acacccggac ggggcgcgtc aagcagttga ccgccggcga ccaggtgcag
    15841 tacggctcgg tggcctggtc gcgcgacgac cgggagatct tcttccgctc caacgaggaa
    15901 aacaagcggg acttcttcat ctaccgcatg gacatcgcca cgggcgagta ccggaagatt
    15961 ttcggtctgc cggggtacaa cggggtggcc gacctctcgc aggacggatc gaagatgatc
    16021 gtctaccagt tcaattccaa cgtggatgac gacctctacc tgctcgatct ggcatccggc
    16081 gagcaccgcc ggattacctc cgacaccagc gacgtaatgt atgattcccc caccctgatg
    16141 ccggacaaca agacgatctg gctcatctgc aacgacaacc ccgacggtct gccgcggctg
    16201 gcccggatga cggtcgatgg cccgcaggtg gagtctgtca cggacggctg gctggatccg
    16261 aagtgggcgg tcgaaggact gggcttctcg cgcgactacc ggttcatggc ggcgatcctg
    16321 aatgaagacg ggtacgcgcg gctgaagctg cgcgaggcgg ctaccggcaa ggagctgaaa
    16381 agcccgccgc tggaggggat cgttgggggc gggctgttcg accgccaggg caactgcttg
    16441 atctcgttca attcgccgac ccgggccccc gacgtgtggc gctggaaccc gcagtcggag
    16501 aagctcgagc agctgacgtt ctcgatctac gcgggcatcg accgcgagtt gtttcgcgag
    16561 ccgcaactgg tgcgctacaa gagttttgac agcctcgaga tcccggcctt cctctatctg
    16621 ccgcccaact acaagcaggg tcagccggtc ccgttcatcg tgaacgccca cggcggcccg
    16681 gaggggcagt accggcccta cttcgagcgc aacctccagt acttcctcct gaacggattc
    16741 gggttgttcg cgccgaacgt gcgcggttcg gatggttacg ggcgcaatta cctcaacatg
    16801 gacaattacc ggaaccgcaa acactcgctc atggactacc gcgcgggcgt ggagtggctg
    16861 atcgccaacg gctacgccgc gccgggaatg atcggcatcc gcggcgcctc ctacggcggg
    16921 tatgtcgtgc tcggcatggt gaccgaattc ccggacctgt tcggcgcggc ggtcgatgaa
    16981 gtcggtatcg ccaatttcca gaccttcctg gagaacaccg ccccctaccg ccgggcgctg
    17041 cgggaatcgg agtacggacc gttgagcgat ccggcgtttc tgcgggagat ctcccccatt
    17101 cacaaagcgc acctcatcaa gacacccctt ctggtcgtgc acggggagaa cgacccccgc
    17161 gtgccggtgg gggaagcgcg gcagatcatc aaagccgtga agcagtcggg cggagtggtg
    17221 gactcgctca tcttcccgga cgagggccac ggcgcaagca agcgggtcaa tatcatcgcc
    17281 gagtaccgca agcaggtgga gtttttcacc cggcacctga aaccggccgg ggaggcgcgc
    17341 aaggagtaaa gagagctgac ggcggccggc gcctgcggat cgggcgaatt gcgcgctccg
    17401 cgggcgcggt ttgccctggc gggtccgtag aatcggccct gttccacctg gcaggcatga
    17461 acctgtcctg ggacatcacc gttttggata agatgttggg gcgcccgaac ggccacggga
    17521 acagcccggg aggaaagcta tgaccgcaca agaaccctgg tacgagcgcg aggatttctg
    17581 ggagtggacg gcgccgttca tgttcggcga ggagcgtttt gagcgcgccg cgtcatcggt
    17641 cggcgagctg acggcgtttt tgcggctggc gtcgccggcc ccggtgttgg acctgtgcgc
    17701 cgggccgggg cggtttgccg ttccgctggc ggcggcgggc tattccgtca ccgccgtcga
    17761 ccgcagccgg gcgttcctcg ccaccctcac cgcccgcgcg cgcgggcgca gtctcgatat
    17821 cgagaccgtc tgctgtgaca tgcgcgagtt ccggcgcgag ggagcgttcg aggcagtcct
    17881 caatatgtac acctcgttcg gatatttcga ggaccctgag gacgaccgga gggtgctgga
    17941 gaacgtcttc agctcgctgc ggcccggcgg gcgtctcgtt atggaactcc acgggcggga
    18001 aagcttgatc cgggcgttcc ggcggcgcga ctgggaggag gcgcacggga tcaccataat
    18061 cgaggagcgg tcgttcgtgg atcacttcac gcgcctgcac aaccgctgga tcgtcctgcg
    18121 ggggagcgag cggcgggaat ttgaattcga tctgcggatc tacggagcct ccgagctgtg
    18181 cgccctcctg cgctcggtgg gattcgcgtc ggccgaggcg tacgccgact tccagggcgc
    18241 gccgtacgac atcgcggccg gacgactcgt tgccctcgcc gctaagtgag gcggtcggcc
    18301 caggtaacgg gccgggtggc cgactcctcc cacggcggca ccggctgccg ctcgcggcgc
    18361 aaagatccgc cgcggcatcc gaggaacgcc agcagcaggt ccatccggtg gaagagggtg
    18421 tgttcccgat agacccgctc catgccggcg cggatgatcg ggaggcgttg gtccggatgg
    18481 ttccggaagt aatcgaacat ttccacaaat cggcccggag aatcggccgc cgcggtctcg
    18541 tcgggggcga agaacgcgcc gccgaaagcg gtgttgtcgg tcagggcaaa ccccccgcag
    18601 gcgggaatcg cgagggcgcg caggttcgcg gcgaaggact cccacgaggg gcccgggagg
    18661 gggagattcg ggcagacgcg cgcgaagttg aacagaaccg cctcctccgc ctggtggagc
    18721 tcgcccagac cgatgtccca gccccgcccg gccagaactc ccgaatacag gcggaggacc
    18781 ggcaggaggc aggctttcac gcgctcgacc gtctggggcg tattgtcgcc cacgaaggcg
    18841 tagtcccaga cctcggcgcg cggcctcata tagtggcgca acgggttgat cgccggcggc
    18901 atgtcaagaa gcggcgtctc ggctccgtcg gcgcgcggaa ggtcggccgg gcaaccgagg
    18961 gcaatcagga aatcgcaggg gggatattcg tgccggaacg acgtgaggtg cccgacgcga
    19021 agcccgtggg cgcggcgata tcgcccaatg tagtcgcggt cgagaccgac ctgcgcagcc
    19081 ggttcggcga gcgtgatgaa gacatcggga cggaactgct cgaagaccgg ttcggtcggg
    19141 tcctcccgcc gcaggaggcg ggcgggaatc ccgccgtagg cgagcacctc ggcccagttt
    19201 tcgatgaggg agcgcagcgc gccgggcggc ggcagatgca ggagaatgcg ggtgtcggca
    19261 tgcacccagc ggttgcgacc ggccgcgaga gcttcctccc gcagccggag attctcccgc
    19321 aggaggggcg cggcggcgca gtccggggca tagcgcgaca gccaatggag agtgcggagc
    19381 aggtcgcgtt tgcggcggag atccggggcc aggcgcgcaa ggacccgccc ccacaggtcg
    19441 cagaggccgc ggtgggctga gcccagcatg gggtcggcgg ccagcgcccg ctgcagcagc
    19501 gcttccgcct ccgcttccag ccccgccgcc gccgcgacct cgccggccac cgcgagggcg
    19561 tcgggatcgt gcggcgcgag tctcatcacc gcccggacgc actcgagagc gccgggagca
    19621 tcgccggcct gcagccggag gcgcgcagcc gccgccaact gcggcacgga gaacagacag
    19681 ggcagagcag cccgacagga agaccgagcg gccgggccgg gccggagtgg tcggaacaac
    19741 ataaccccca ttttggctct gggtccgatc taagcggtat atcggcagat gggacggggg
    19801 aattcaacgg agcggctctg atgtcggggc gcgcgaccgc atcgggcccc tctcccccgc
    19861 gcgggcggct actcgccgat gattttgacg atgacgcgct tgcgccggcg accgtcgaat
    19921 tcggcgtagt acacctgttg ccacggcccg aggtcaatgc ggccgtcgct gaccggcagt
    19981 aagacttggt ggtgcagcaa caggttcttc agatgcgcgt cggcgttgtc ttcgccggtg
    20041 cggtggtagc ggtaatcgtc gcggtgcggc gccagcttgt ccagccaggc gtcgatatcc
    20101 tggatcagcc ccggctcggc gtcgttgatg taaaccgacg cggtgatgtg catggccgag
    20161 accaggatca agccctcccg gatggcgctg cgctgcagag cctgctcgat gcttgccgta
    20221 atattgatgt actcccgtcg gcgggaggtg ttgaactcaa ggaattcgcg gtagtgcttc
    20281 atggggtgga tgcggcgcgc gctgcacgga acacggctac ccgacggtta cctgatccgg
    20341 tcgatccagg tgtcaaacga gtacttgtcg ctcagctcct gctgcacttg ccgggcctcg
    20401 gcgtaggtcg gatagacccc cagccgtacc cggtggtagg tctcgccccc gacagtctcg
    20461 gtgctcacaa agggctcgta gccgcgctcc cggaacttct cgaccagctc ggcggcgtaa
    20521 tcgggatctt cgcaccccgc gagctggacg acgaaaccgt cgccggcagg cgggaccggg
    20581 aggtcaaccg gggtcagggg ctggtccggg gtcgtttccg gtgcgacatc ctgcgcctcg
    20641 accggcgtct cattgagagg ctcgtccggg ggctcgtcgg gaatcgcgcc ggcgttcagt
    20701 tcggggcgga ccgaatcggg ggctgaggcc gccgccggca gcacggggac gtcggaaacc
    20761 gccgcggggt gggagtcggt ctgtccctgt tgcgccatca cctcccgctc cagctcgaca
    20821 atgtcatcgt ctttcgaaca gccggcgagc ccgcaggcgg cggccagaat caggcagcaa
    20881 agtatcccca gggatctcac agtacctcca aatcagtccc gccggcgccg gggcgccgca
    20941 gttaggtcta cgcgatgctt ccggccgata ctagggcgcg cggccgggat tgtcaacgaa
    21001 aaagcgtttg tccggggggc gatgaaaata gttgaaattt gtataagaaa tgcctacgct
    21061 tgacgagtaa aagcaagagg cgtatgagga cagtagaaca cattctcgac ctggtgggcc
    21121 acaccccgct ggtgcggctg cggaccggaa tgaccgatcc cggaccgcgg gcgtacgtga
    21181 agctcgagtt cttcaacccg accggctcgg tgaaagaccg catggcctac tacatcatca
    21241 ccaaggcgct gcgggacggc cggctgaaac cgggcgacac cgtgatcgac aatacctccg
    21301 gcaacaccgg ctcggcggtg gcgatggtct gctccgtgct cggactgcgg gccgtgctga
    21361 cgacgccggt gaagacgagc caggagaaga ttgacctgat caagtcgttc ggcgctgagg
    21421 tgatcgtgac gcgggagacg gaggatcacg ccgacccgga gggagcctac atggtggccc
    21481 ggaggctggc ccgcgagcac gggtacttcg acctcgacca gtacaacagc caggagaatg
    21541 tcgaggccca ctaccgcctg accggtccgg agatctggga cgacaccgaa ggccggctga
    21601 cccacttcgt ggccgggatc ggaaccgggg ggacgttttc cggcgtcgcg cggttcctca
    21661 aggagaagaa tccggcgatc aaggcgatcg cggtcgaccc ggagggttcg gtgttcgccg
    21721 cctatatccg cagcgggcgg accaccaccc ccgcggcgta caaaattgaa gggatcggct
    21781 ccgacgtgat caccgaggcg ctccatccgc aatatatcga cgaggtcatt accgtttccg
    21841 accgcgacgc tttccagcgg gcgcggctga tcgcgcggac cgagggcatc tccggcggcg
    21901 ggtcatcggg cgcggtggcg gtcgcaattg agcaagtggc ccgcagggca cggccggagg
    21961 atattattgt cggtattttc gctgacgccg gcatccgcta cctcagcaag tgctacaacg
    22021 acagttggat gcaccagcac ggcttcctgc cggcggggat cccggagaag acatgacgcc
    22081 gaagggtaga gctctgatat cggccgtcgc tttcgcggcg gcgcttccgt tggccgcttg
    22141 gaccgggacc cagtcgccgg ctcccggcaa ggggctggac ttcccgaccg ccgttttcga
    22201 cttcgggcac atcggcatcg actacaccgt ccgtttccgc taccccgtga tcaaccgctc
    22261 gccggacacg gtgcgcgtgc gcaaagtcga tgtgcactgc gattgttcgg cagcctttgt
    22321 cgccgacagc gttctggcgc ccggcgattc gaccaacatc cacctgcggt ttaatacccg
    22381 ggacttctat ggtccccaga ataagagcct cacaatcacc accgaccggg aaggacaatc
    22441 gactatcgac ctttactacc tgtcgattat tgggcaatgg ttcaacggga tcaagccgga
    22501 tc
//
LOCUS       DAOPOE010000050        22370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_2891_length_22370_cov_5.739771, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000050 DAOPOE010000000
VERSION     DAOPOE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22370
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_22370_cov_5.739771"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="dnaX"
                     /locus_tag="PKM94_08045"
     CDS             complement(<1..1260)
                     /gene="dnaX"
                     /locus_tag="PKM94_08045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD66631.1"
                     /translation="MTYQVLARKYRPPTFEGVVAQAHVTRTLQNAVRNGRVGSGYLFC
                     GPRGTGKTTTARILAKALNCVNGPTPEPCGVCPACRDITGGNSLDVLEIDAASNTGVD
                     DVRALRERINLTPAGGKKKIYIIDEVHRLSGAAFDALLKTLEEPPEHVVFIFATTEPT
                     KVPETILSRTQRFDFKRVSALDLAQHLQHIAEQEGMAISESAVALLARRGDGSVRDAL
                     SLLDQVAAFAGETIGETDVVEALGLVDRQTLFDFTGAVAAQDAPAALRLANGIIEAGI
                     DVEDFFAEALDHLRILMILLTDRESGDLLNLSAEEREAYLRQADSFRVGDVIRLMKMV
                     GDGRRDLKDGLDERLVLQVAAVKMAAMEATVQFEEVLSHLRQNPPAASGTADLFGNPA
                     KKKDPDRPPEPRGGVSAPPTPPIDPQRT"
     gene            1401..2015
                     /locus_tag="PKM94_08050"
     CDS             1401..2015
                     /locus_tag="PKM94_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66632.1"
                     /translation="MRQQLRRALVLGLLLLPASASAAPTRADGVPKVIAHGGVGLKLG
                     LINTGALTLTGRPTPWDSVAVAGKELDPKAAPSGQVFFDVPLTRAFLLLFSVDIYDLR
                     FHPLSERFIDVAAGFKLAFYKHHQRLAWRPGAAVGLGYLADIGFMRPTTYLTLKAFSE
                     LVLLPRGKWAVVLELGFMAAPSGGNSAVDVKIAPSLFLRAGLLY"
     gene            2102..2174
                     /locus_tag="PKM94_08055"
     tRNA            2102..2174
                     /locus_tag="PKM94_08055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2135..2137,aa:Ala,seq:cgc)
     gene            2263..2526
                     /gene="ffs"
                     /locus_tag="PKM94_08060"
     ncRNA           2263..2526
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKM94_08060"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2655..4532
                     /locus_tag="PKM94_08065"
     CDS             2655..4532
                     /locus_tag="PKM94_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66633.1"
                     /translation="MRRSLLFSLAALAILLGGLALTGRAADKIKIEKLDDLPRHTYRI
                     AVKAAEVPVNPEALAALAAAVKADLLADLERYEIPDATTIKNYHSDLGAIALLEADFA
                     AYETHIAAVRDLEDKEAQKLTTGLTGRAYAAAMAAPEAQRPEAYRTAYAALVNALPYD
                     VVEAQLRSTKGMAEMMSENLVLGLAESKYQPLVDSTGGVISKDVALSLLKTATMLRTI
                     IPYRESIAGVIDAYLAAHAVDKPDIWAERQVALSGSEGGQPVLVTIWDSGLDTEIPAI
                     AQVLWTNADETPGNGSDDDGNGFVDDVHGIAYTLHSDKTPDLLYPIGEVGAERPRLQR
                     QMKGLTDISMAVDSKEAAELKQELSGLQQAEAQTFIQNIALYGNYCHGTHVAGIAMAG
                     NPYIRLMPSRLTFGYTLVPEKPTLEQARKDSAAADEAIAYYRKHGVRVVNMSWGGSLA
                     SVEQALEAHNAGGTPEERRELARQIFEIGRSGLYAAMQNTPEILYVTAAGNEDNDVKF
                     EEVIPSAFDLPNIIVVGAVDQAGDETGFTSFGNVDCYANGFEVESFVPGGDRMKLSGT
                     SQASPQVVNLAAKVLALKPELTPAQVRELILSGCDEKQVGDRAVKLINPKKTMGLATP
                     R"
     gene            4661..5230
                     /locus_tag="PKM94_08070"
     CDS             4661..5230
                     /locus_tag="PKM94_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD66634.1"
                     /translation="MTDRSDIGLTREEAFRLVLEKIGHTNLLKHILAVEAGMRRLAER
                     LGEDVGLWGLTGLLHDLDYNETVGDAGAHTFVTESWLQPYHLPAAMLYAIRAHPGHVP
                     CRSRLDWALFATDPATGFIVACVLMHPAKRLDVIDADFMLRRFKEKRFAAGASRDNMA
                     ACTSLGLELEEFLLLVHAGMMTIRSDLQL"
     gene            5239..6558
                     /locus_tag="PKM94_08075"
     CDS             5239..6558
                     /locus_tag="PKM94_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD66635.1"
                     /translation="MASTIGRKDVIGWSLYDLANTIYSMNITSLYLKRYIVEDLGRDD
                     RWFDIPFALSMFLAALLLPAFGAMSDHSVKKKLFVFLFTLTCCLAVGGMALVPPGALI
                     VLVALFVLANFSYEAGQPFYNALLYSVADGPAARFVSGFGVSLGYVGSILGMVLVLPF
                     VSGELFGLTVPFVTPGGKAGAFVPTAVMFLLLSLPLFLWVRERPSPSPQASGLARAYR
                     EVWEGIRNTKKYPGVLRFLIADYFFEDAVATVVINIGLYCSVVLALDDSRITAFLIIS
                     TVSAVIGSVVIGKIAERVVLKRLLAGIVWLWIAALVVFVSTDTMTVVWILGSVVGVAL
                     GGLWTVSRPLLGELVPHDELGRFFGLFSISGRAAAVAGPLIWTSSVYLFRADRPLGRF
                     ASDLLALSPSGQAKLPYKVAVLSLVVMMAVGLIIFRKVPRTERSRHG"
     gene            6551..7603
                     /locus_tag="PKM94_08080"
     CDS             6551..7603
                     /locus_tag="PKM94_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66636.1"
                     /translation="MDKRKVGDWYHYTGAVHIHTTESDGTLPLEEVIALGREVGLDFM
                     MFADHMTLSNRQRGGEGMYGDTLVVIGYEHNDERDRHHYLLFGSPGVYPPSLSARQYV
                     AAGAADGAIGIMAHPDEIRNSMPEFPPYPWDDWSVEGFTGIELWNQMSEWMERLTPRN
                     RLAMAFAPRRSMIGPSDRILRRWDEISRKRKCVGIVGADAHAFPIKVGPFRFAIFPYK
                     VHFKCLRMTIILPEPMSRDFVAARDQLYEAIRDCRAFGANVRWGQADRFLFCADNGRR
                     RVPSGGTIELTETAHMRIELPARARIRLVHDGCTTVENEGQELIHRLDAPGIYRVEAW
                     KGRRGWIFSNHIRVEA"
     gene            7600..8007
                     /locus_tag="PKM94_08085"
     CDS             7600..8007
                     /locus_tag="PKM94_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66637.1"
                     /translation="MSKWEADRVKVVDLAGTEAPDSRTVIRCKFVLIELADEIHLVFG
                     RLADYPYHARLVAQFCSERRIPSGWEHEPDLYALYTANARVCGGGYLAIDPACRTAEV
                     YGRSNAYGPYDKKAMHAVVEHGPVFRGLTVSVR"
     gene            8212..8568
                     /locus_tag="PKM94_08090"
     CDS             8212..8568
                     /locus_tag="PKM94_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOD66638.1"
                     /translation="MFDTFFPILLLVVFAVGIAAVLVALSVIFGRRTVLGRKTDPYEC
                     GVEPVGTTRNPVPVKFYLIAISFILFDIELIFLLPWAVVARDLGSYGFWAMAVFVAII
                     VVGYLYELRRGALKWD"
     gene            8559..9011
                     /locus_tag="PKM94_08095"
                     /pseudo
     CDS             8559..9011
                     /locus_tag="PKM94_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            9112..9651
                     /locus_tag="PKM94_08100"
     CDS             9112..9651
                     /locus_tag="PKM94_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOD66639.1"
                     /translation="MKSETSEGNDARIRRFLRERFGEAIRHEEVFRGDLQFTVDPEKI
                     YDICEALMEAGGLDVRFLADITCVDWHGHSEAVHGRFEVVYNLCSLAHRSRFFLKVRL
                     PETNPHVRSLVDLWHGANWMEREVWDLFGVTFDGHPDLTRLLTPDDFEGHPLRKDYPL
                     VYEQPKFSWNENDPPEVIR"
     gene            9651..10823
                     /gene="nuoD"
                     /locus_tag="PKM94_08105"
     CDS             9651..10823
                     /gene="nuoD"
                     /locus_tag="PKM94_08105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOD66640.1"
                     /translation="MAEARTVTINMGPQHPSTHGVLRVILELDGETVVKARPVVGYLH
                     TGIEKTMESKLYYKALPCTDRMDYLAPMSNNLGYSLAVEKLLGIEVPEKVKWARVILA
                     ELTRINSHLVWLGTHALDLGAMSMLLYAFRERELILDVYEACGGQRMMTSYIRIGGLA
                     HDLPKDFDRKVRDIIATLTARLEDYEGLLTRNEIFINRTKGVAVISAEDALNWSLSGP
                     MLRGSGVARDLRKDEPYSGYENFDFQIALGHNGDAYDRYLLRMEEIRQSLRICRQGID
                     GLPEGPYRAHVPGVVLPPKEDVLYKMESMIFHFKIITEGFAAPLGSVYQAIESPKGEL
                     GFYVVGDGTTMPRRIRVRPPSLINLASLPRLVEGRLFSDVICAIGSIDIVLGEVDR"
     gene            10820..11296
                     /gene="nuoE"
                     /locus_tag="PKM94_08110"
     CDS             10820..11296
                     /gene="nuoE"
                     /locus_tag="PKM94_08110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOD66641.1"
                     /translation="MILSDESVRKIKEKVPLYPRRKSSVLPALTIAYQQLGYVDDDIY
                     REIARVIGVPYVEVAEAASFYTMFPKAPRGKYLIQVCHNLSCALLGADSLIAYLEKSL
                     GIRKGETTPDKLFTLISVECLGSCATAPMMQINNDFYENLTPAKVDQILNDLRSQA"
     gene            11296..12633
                     /gene="nuoF"
                     /locus_tag="PKM94_08115"
     CDS             11296..12633
                     /gene="nuoF"
                     /locus_tag="PKM94_08115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOD66642.1"
                     /translation="MFYSPVITNAAEAADNKKLHLFRYVEDPEQYKLAAYERHGGYRA
                     WRKVLAGMKPDEVIEVVKKSGLRGRGGAGFPTGMKWGFIPKDSPKPRYLCCNADESEP
                     GTCKDRVLMERDPHAIIEGMAIAAYAIGCHIMFIYIRGEFGHPIARMEAAMKEAYAAG
                     HLGRNIHGSGYDLEMVVHTGGGAYICGEETALLNSLEGERGMSRIRPPFPAIEGLYAC
                     PTIVNNVESLAAVPHIIANGPEWYRSMGTEKSAGTKIFSLSGHVNRPGNYEVELGFPL
                     KKLIFDPAYGGGLRDGRPLKGVIPGGSSTPFLTPDAVETVNLDYESLQAAGSMLGSGA
                     VIVFNEDTCIVWLILRLIHFYRHESCGKCTPCREGTAWLEQVIRRIEQGQGKPGDLEL
                     IEDVCGNILGRTICPLGDAAVMPIQSAVKHWRDEWRYHIDNGRCLVKSEFAFK"
     gene            12640..15210
                     /gene="nuoG"
                     /locus_tag="PKM94_08120"
     CDS             12640..15210
                     /gene="nuoG"
                     /locus_tag="PKM94_08120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOD66643.1"
                     /translation="MAQDVKDTQPQKKAEIPTVTLTIDGRPVTVPRGTTVLEAARQMG
                     IHIPTFCWHPKLKPVGACRICYVEIEKFPKLMVSCATEATEGMIVHTDSDLVKQGRKA
                     VLEFILLNHPLDCPTCDKGGECDLQNLTFRHGYDDSRFEFQKYRFTSGAEGSTFDDKR
                     IGPEIILNRNRCILCFKCVRANKEAFGEYDLGAYERGYITEIDAAPGEQVDNPFSGNL
                     VEICPVGALTNTDWRYKIRVWLTQTTPSICNFSATGANLLFYKEDHKNRIYRVTSRRN
                     DGVDDGWLADITRYGYQIAHSEERLHTPLVRRDGKQVPASWEEALEVIRKRFGEITET
                     KGRVCIGGLAAPHLDNAALYAFSKLFRTVLKSNNVDYRSDYRMLPSAADSPFNLLCRR
                     PFRIADIDASDVIVTLGSDLLREHQLEYVRIRKACTFRGARYFSMTPYAVRSADIAEA
                     ELVYAVGMEEVLLTALGLAAVDMGLVDPSAGAVFRARVEPKTLAEACRLCGVAAETVK
                     LAARALATAGRVTFFTGEIVTRSYARDAIGAAALNLDRLLGLHRRGQIAALARYANSA
                     GAERLGVSPTPAPAVAKRLAELWGEFPEAEPGTTDRMMVQMRRDEIDGFFILGANPVM
                     LYPDRQFALESLEKLDFLVAADLFETPTTELADVVLPLASWAEFTGDYVNLEGRVQRA
                     ERAIKPLHQAKPPLEIVRLVADKLGGKLFASEEQMQAEIDAVLAVDATAAPSGEFFEV
                     KPAAEPASEEYPLPLFMGDDPHHSGHLTEKSVALANFCSEAYLEMSPELARRYGLDEG
                     DPVRIESELGKVVAPARISDVLDSDVLLAPRNFASTPVNSLLTRKRRIDRVKISKVID
                     "
     gene            15216..16199
                     /gene="nuoH"
                     /locus_tag="PKM94_08125"
     CDS             15216..16199
                     /gene="nuoH"
                     /locus_tag="PKM94_08125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOD66644.1"
                     /translation="MLEAIIIAAVKVLAVLVAVLTGCAYATYMERKVLGHLQHRIGPS
                     YAGPYGLLQPLADAVKLIFKEDVVPANAERVTYTLAPIASFVPAMLTFAVIPFTADFE
                     LFGRPIQGVISDLNIGILWVFAVTSLGVYGIVLAGWSSGSKYSLLGGVRSSAQMISYE
                     ISYGLTIVGVILIANTLSMRELVEQQASIADWFIWKQPLGFLLYLTCAVAETNRAPFD
                     LPEAESELVAGYHTEYSSMRFATFFISEYGAMIAVSCVGTTIFFGGWQGPFLPPFLWF
                     FLKVLAFMFLYIWLRATFPRFRYDQLMAFGWKVLFPLALLNSLVTAAFVML"
     gene            16218..16724
                     /gene="nuoI"
                     /locus_tag="PKM94_08130"
     CDS             16218..16724
                     /gene="nuoI"
                     /locus_tag="PKM94_08130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOD66645.1"
                     /translation="MKDVLTALTNAVIKTPKGLLITLKHLFKKPVTVDYPRQKRPMAP
                     RYRGRHYLERYDDGTERCVCCGLCAAACPADAIYMEPEENEKGERRARTYEINLLRCI
                     FCGYCEEACPEEAIFLGQDYEFSSSDKSSFIWKKDDMLVAHPRRDNPFKRLYRRVRKV
                     YGVADKVR"
     gene            16721..17326
                     /locus_tag="PKM94_08135"
     CDS             16721..17326
                     /locus_tag="PKM94_08135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOD66646.1"
                     /translation="MSLDAIIFVLSAVVAVFGATMMIVQRNPVASVLYLIVSLIAQAV
                     LYIQLGTAFMGAVLVIVYAGAILVLFLFVIMLLNLRGREDLGEGSAPLSRLTKYLVSL
                     LFVVELVFVIRAGFFAETAVVGVFAGGAVADDFGSVEHTAGLLFTRYLYPFELTAALL
                     LAAVVGAVAIAKRDRAEGPPAGAETRGRAEDAPPQTHKVES"
     gene            17328..17630
                     /gene="nuoK"
                     /locus_tag="PKM94_08140"
     CDS             17328..17630
                     /gene="nuoK"
                     /locus_tag="PKM94_08140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOD66647.1"
                     /translation="MVPVSAYLIVAAVLFGIGTIGVLASRNMIILIMSIELMLNAANL
                     ALIAFSRALNVMDGHAFVFLVLTVAAAEAAVGLAMVLTVFRNRDSINIDRFNLLKW"
     gene            17644..19608
                     /gene="nuoL"
                     /locus_tag="PKM94_08145"
     CDS             17644..19608
                     /gene="nuoL"
                     /locus_tag="PKM94_08145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOD66648.1"
                     /translation="MSDYLFLIPLLPLIGFVVNGLLLGRLRRPAVNAVACASVGGAFI
                     LSVLAFFELRALPPAMRMIEQTLFTWIPSGNFNVNIGFLLDPLSAVMILVVTGVGFLI
                     HVYATGYMAHDPGFGRFFAYLNLFMFAMLTLVLADNFLLMFVGWEGVGLCSYLLIGFW
                     YERQSAADAGKKAFLVNRIGDFGFLLGMFLIFWQVGSLHFPTVSEAAPAVFTAGGGLI
                     TAVCLLLFLGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMIARTNVLY
                     SLASDALLVVAVIGAATAFVAATIGLAQNDIKRVLAYSTVSQLGYMFLACGVASFTAG
                     IFHLMTHAFFKALLFLGAGSVIHAMSDKQDMREMGGLRKHLPVTYATMLVATLAIAGI
                     PGFSGFFSKDEILWKSFSSAHGHPLFWVIGFATAGLTAFYMFRLVFLTFFGKERMDQH
                     TKDHLHEFPRSMTVPLVILAVLSVVGGYVGIPHVFGATNWFERWLEPVMAGGGGEAAP
                     ETVHASAGGYGTGMELALMGASVALVLLAIYGAHLFYRKRTELATRWRERLSGLHRLL
                     VNKYYVDEIYLAVIVRPLVGLSMFLWKFVDVIVIDGLANGSAAAYQGLSEGVRRLQTG
                     RVRSYATLFVLGVVLLVAYFAMDWGWKIKF"
     gene            19590..21188
                     /locus_tag="PKM94_08150"
     CDS             19590..21188
                     /locus_tag="PKM94_08150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOD66649.1"
                     /translation="MENQILTYVTFFPLVGIVALLLAPKERHDTIKAVALIVAVITML
                     LSFFLWSMFDPMALGMQFEVDIPWITGLGIHYHLGIDGISLLLIALTTVLTVLAILAS
                     WNSITKGVKGYYLSMLLLQVGMIGVFVSLDLFLFYVFWEIMLVPMYFIIGLWGGPRRV
                     YAAIKFVLFTMFGSLLMLVGILWLLFAYHGYSGEYSFDMLKLVEMPIGYRAQLLLFGA
                     FALAFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFVRICLPMFPDATV
                     AYLPYIAVLSVIAIIYGALVAMVQRDVKSLVAFSSVSHMGFIMLGIFALNLQGLQGAI
                     MQMVNHGVSTGALFLLVGMIYDRRHTRLIEEFGGLAKVMPAYSAIFMIVALSSIGLPF
                     TNGFVGEFLILLGAFDRSYVYGTLAASGVIFAAAYMLWMYQRVIFGKVTNPANAQLAD
                     VDWREKIILIPLVIFIFWIGIYPKPFFERIEPAAQQVLTRVGRAQTVQLDDQFRVNLV
                     PAVDISPRGDTITVGLGAPERGTE"
     gene            21185..21397
                     /locus_tag="PKM94_08155"
     CDS             21185..21397
                     /locus_tag="PKM94_08155"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOD66650.1"
                     /translation="MNNVVMYTKEGCPYCAAARKHYAEQGIRFTEIDIHRTPGARDKV
                     LALTGGEAIVPVIVDNGQVIKGFGGG"
     gene            21443..>22370
                     /locus_tag="PKM94_08160"
     CDS             21443..>22370
                     /locus_tag="PKM94_08160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOD66651.1"
                     /translation="MIDLTSAGIDFGLIGPETALLIAASVILLTGYLGRLGRFSPGLS
                     LVGLSVALALAAASWGRQGSGFWGMVTNDNFGSAFKVIAAGTSILAQLMAQRWLAVKG
                     YHRPEFYALLLLATMGMMVMANTTDLVVLFLGLELMSVPLYVMAGFAKRSLESNEAGL
                     KYFIMGGFASAFLLMGIAFVFGAAGTTDLRRIVADFNFILSRSDAYMYAGAALILIGF
                     GFKVAAVPFHSWVPDVYQGAPTPVTAFFSVGPKAAGFAALLRVFVFGFASMEALTDAF
                     WVLAVLTMTVGNVLALRQSNIKRLLAYSSIAHA"
BASE COUNT         3741 a         7474 c         6985 g         4170 t
ORIGIN      
        1 cgttcgctgg ggatcgatgg gcggcgtcgg cggagcgctt acgcctcccc gcggctccgg
       61 cggtcggtca gggtcttttt tttttgccgg gttgccgaac aggtcggccg tcccggaggc
      121 cgccgggggg ttctgccgaa ggtgagagag cacttcttcg aactgcaccg tcgcctccat
      181 ggccgccatc ttcaccgccg ccacctgcag cacgagccgc tcgtcaagac cgtccttgag
      241 atcccgccgc ccgtccccga ccatcttcat cagccggatc acatcgccga cccggaaaga
      301 gtcggcctgc cggagatagg cctcccgctc ctcggccgag agattgagca ggtcgcccga
      361 ctcgcggtcg gtcaggagga tcatgaggat gcggaggtga tcgagcgcct cggcgaagaa
      421 atcctcgacg tcgatgccgg cctcgatgat gccgttggcc agccggagcg ccgccggcgc
      481 gtcctgcgcc gccaccgcgc ccgtaaaatc gaagagcgtc tgccggtcaa ccaggccgag
      541 cgcctcgacc acgtcggttt ccccgatcgt ttccccggcg aaagccgcca cctggtcgag
      601 aagcgagagg gcgtcgcgca ccgaaccgtc cccccgccgc gccaggagcg ccaccgcgct
      661 ctcgctgatc gccatgccct cctgctcggc gatatgctgg agatgctggg ccaggtcaag
      721 cgcggacacc cgcttgaaat caaaccgctg cgtccgggag aggatcgtct ccggaacttt
      781 cgtcggctcg gtggtggcga aaatgaacac cacgtgctcc ggcggttcct cgagcgtctt
      841 cagaagcgcg tcgaaggccg cccccgagag ccggtggact tcatcgatga tgtagatttt
      901 ctttttgccg ccggcggggg tcaggttaat ccgctcgcgc agagcccgga catcgtcgac
      961 ccccgtgttg gaggcggcgt cgatctccag cacatccagc gagttgccgc cggtaatgtc
     1021 gcggcaggcg gggcagaccc cgcacggttc gggggtcggg ccgttgacac aattgagcgc
     1081 tttggccagg atccgggcag tggtggtctt cccggtgccg cgcgggccgc agaacagata
     1141 acccgatccc acccgcccgt tgcgcacagc gttctgcagc gtgcgggtca catgtgcctg
     1201 tgccacaacg ccttcaaacg tcggcgggcg atacttccgg gccagaacct gatacgtcat
     1261 aactctccag tatatgcaat atctgcctca ttcccaatca caatttttcc gcgtccgaag
     1321 ggcgccgcta ttcgtattga caaacagcag gtaattcctg tctatgtggc gagaaaatcc
     1381 tctatcgaaa ggcccccctt gtgcggcaac aacttcgacg cgccctcgtt ctcggcctgc
     1441 tcttgctccc ggcctccgct tcggcggcgc cgacgcgggc cgacggagtg ccgaaagtca
     1501 tcgcccacgg cggggtgggc ctgaagctgg ggctgatcaa caccggcgcc ctgactctca
     1561 ccgggcgacc cacgccctgg gattcggtgg ccgtggcggg caaggagctt gatcccaagg
     1621 ctgctcccag cggacaggtg tttttcgatg tccccctgac ccgcgctttt cttctcctat
     1681 tctccgtcga catctacgac ttgcggttcc accccctcag cgagcggttt atcgatgtgg
     1741 cggccgggtt caagctcgcc ttttacaagc accatcagcg gctggcttgg cggccggggg
     1801 ccgcggtcgg gctcggctac ctggccgaca tcggcttcat gcgccccact acgtacctca
     1861 ctctcaaggc gttcagcgaa ctggtgctcc tcccgcgggg gaagtgggcg gtcgtgctcg
     1921 agctcggttt catggccgcg ccgagcgggg gcaacagtgc cgtggacgtg aaaatcgcac
     1981 cctccctctt tctccgggca ggactcctct actaggagag ggggagagac ggggccggaa
     2041 aaaatcgctt ggcaaatcct tccgggacga ttatagtatg ggccgtgatg tgaagatccc
     2101 tggggcagta gctcagttgg gagagcgcaa cgttcgcaac gttgaggtcg tcggttcgat
     2161 cccgatctgc tccacttaaa aatagcgcgt gcgcgacagc agtggaccgg cctcccgcaa
     2221 ggaggccatt gttgtcaccg gctgttcgct cgtgcgacga gcctgtgcta ggcggggagt
     2281 tagcggtgcc ctgtacccgg aacccgctat accggggccg aattcccacc cgcgggtgcg
     2341 gtcgccggac ttcgccaacg ggtaagtggt gttgaaggct gggtcccgta caacggaaac
     2401 ctatgaaccc ggtcaggacc ggaaggtagc agccgtaagt agtccgttcc gtgtgttgcg
     2461 ggagcgcccg gttggagcca actgcctcga gggttgtccg ggggcggctc aacggtgggt
     2521 gcacagtaaa atcgcggccc gtcggtttcc ccggcgggcc gtcttgtttc ccggccgccc
     2581 gcaaccaaac cccgccccga aacgtacaaa cgcagcaata acatggtgcg acacgtctga
     2641 cataaggagc ccgcatgcga cgttcactgc tcttctccct cgccgctctc gcgattctgc
     2701 ttggcggcct ggccttgacc ggccgcgcgg ccgacaagat caagatcgag aagctcgatg
     2761 acctcccccg ccacacctac cgaatcgcgg tcaaggccgc tgaggtcccg gttaatccgg
     2821 aggcgctcgc cgcgctcgcg gcggccgtga aagcggatct gctggccgac ctcgagcgct
     2881 acgagatccc cgatgccacg accatcaaga actaccactc cgacctcggc gcgatcgcgc
     2941 tcctggaggc ggatttcgca gcctacgaga cccatatcgc cgccgttcgc gacctggagg
     3001 acaaggaggc gcagaagctg acgaccggtc tgaccggccg ggcttacgcg gcggctatgg
     3061 cggcgccgga ggctcagcgc ccagaggcct atcggaccgc ctacgccgcg ctcgtcaacg
     3121 ctctgcccta cgatgtcgtc gaggcccagc tccggtcgac caaggggatg gccgaaatga
     3181 tgtccgagaa cctcgtcctc ggactggcgg aatcgaaata tcagccgctg gtcgactcga
     3241 ccggcggcgt catcagcaag gacgtcgcgc tctcgctgct gaaaaccgcc accatgcttc
     3301 ggacgatcat cccctaccgg gaatccatcg ccggggtgat cgacgcttac ctggctgcgc
     3361 acgcggtcga caaaccggac atctgggccg agcgccaggt cgcgctcagc gggagcgagg
     3421 ggggacagcc ggtgctcgtg accatctggg actccgggct cgacaccgag atccccgcga
     3481 tcgcccaggt gctgtggacc aacgcggacg agacccccgg caacgggagt gacgatgacg
     3541 ggaacgggtt tgtcgacgat gtgcacggca tcgcctacac gctccactcc gacaaaaccc
     3601 cggatctgct gtacccgatc ggcgaagtgg gggcggagcg cccgcgcctg cagcggcaga
     3661 tgaagggact gaccgacatc agcatggccg tggacagtaa ggaagcggcg gagctcaagc
     3721 aggaactgag cgggctgcag caggccgagg cccagacgtt catccagaac atcgccctct
     3781 acggcaacta ctgccacggc acacacgtgg cgggaatcgc catggccggc aacccctaca
     3841 tccgcctcat gccctcccgc ctcaccttcg ggtataccct cgtccccgag aaacccacgc
     3901 tcgagcaggc ccggaaagac tccgccgccg ccgacgaagc gattgcgtac taccggaagc
     3961 acggcgtgcg ggtggtcaac atgagctggg gcgggtcgct cgcgagcgtc gagcaggcgc
     4021 tcgaagcgca caacgccggc gggacgcctg aggagcgccg ggaactggcc cggcagattt
     4081 tcgaaatcgg ccggagcggg ctctatgccg cgatgcagaa cacgccggag atcctctatg
     4141 tcacggccgc cggcaacgag gacaatgacg tgaaattcga ggaggtcatc cccagcgctt
     4201 tcgacctccc caacatcatc gtcgtcggcg ccgtcgacca ggccggcgac gaaaccggct
     4261 tcacctcctt cggcaacgtc gactgctacg ctaacggttt cgaggtcgag agtttcgtgc
     4321 ccgggggcga ccgcatgaag ctctccggca cctcccaggc ctcgccgcag gtcgtcaacc
     4381 tggccgcgaa agtcctcgcc ctgaagccgg agctcacccc ggctcaggtc cgcgaactca
     4441 tcctcagcgg ctgcgacgaa aaacaagtcg gggaccgggc ggtaaagctc ataaatccca
     4501 agaagacgat gggactggcg acgccgaggt aggttctctg ccccggcggg gaaaaaccgc
     4561 gccgccggac cggtggtccg gcggccgcct gcggcgggga gcgggcgggc cgccgcatcg
     4621 ttccttgaca cgcttctgcg cagggccttt attgggcgtc atgaccgacc ggagcgacat
     4681 cggcctcacc cgcgaggaag ccttccggct cgtcctggag aagatcgggc acaccaatct
     4741 cctcaaacac attctcgccg tggaggcggg gatgcgccgg ctggcggagc ggctgggcga
     4801 ggatgtcggg ttgtggggcc tgaccgggtt gctccacgat ctcgattata acgagacggt
     4861 cggcgacgcc ggggcgcaca ccttcgtcac ggagagctgg ctgcagccct atcacctgcc
     4921 ggccgcgatg ctctatgcga tccgcgccca ccccggccac gtgccatgcc gcagccggct
     4981 cgactgggcg ctcttcgcca ccgacccggc gaccgggttc atcgtcgcct gcgtgctcat
     5041 gcatccagcc aagcggctcg acgtgatcga cgccgacttc atgctgcggc gcttcaagga
     5101 aaagcgcttc gccgccggcg cctcgcgcga caacatggcg gcctgcacca gcctcggact
     5161 cgaactcgag gagttcctcc tcctggtgca cgccggaatg atgacaatcc ggagcgacct
     5221 ccagctatag gactgcgcgt ggcctcaact atcgggcgga aggacgtcat cgggtggtcg
     5281 ctctacgatc tcgccaacac gatctactcg atgaacatca cttcgctcta cctcaaacgg
     5341 tacatcgtcg aagacctcgg ccgcgacgac cgctggttcg atatcccgtt cgctctttcg
     5401 atgttcctcg cggccctgct tctgccggcc ttcggggcga tgtcggacca ctcggtcaag
     5461 aagaagctct tcgtgtttct cttcacgctc acctgctgcc tcgcggtggg ggggatggcc
     5521 ctcgtgccgc cgggagcgct gatcgtgctg gtcgctctgt ttgttctggc caacttctcc
     5581 tatgaggccg gccagccctt ctacaacgcg ctgctgtact cggtggcgga cggcccggcg
     5641 gcgcggttcg tctccggctt cggcgtgtcg ctggggtacg tcgggtcgat tctcggcatg
     5701 gtgctggtgc tgccgtttgt cagcggcgag ctcttcggac tgacggttcc tttcgtcacg
     5761 cccggcggca aggcgggggc gttcgtgccc accgccgtga tgtttctgct gctctcgctg
     5821 ccgctcttcc tctgggtgcg ggagcggccc agtccttcgc cgcaggcttc cggcctggcc
     5881 cgggcctatc gcgaagtctg ggagggaatc cgcaacacga agaaataccc cggcgtgctc
     5941 cggttcctca tcgccgacta ctttttcgag gatgcggtcg ccacggtcgt catcaacatc
     6001 gggctctact gctccgtggt gctcgcgctg gacgacagcc ggatcaccgc gtttcttatc
     6061 atctcgaccg tctcggctgt gatcggatcg gtggtgatcg gcaagatcgc cgagcgggtg
     6121 gtgctcaaac ggctcctggc cggcatcgtg tggctctgga tcgccgcgct cgtcgtcttt
     6181 gtatcgaccg atacgatgac ggttgtctgg atcctcgggt cggtcgtcgg ggtggcgctg
     6241 ggcgggttgt ggacggtctc gcgcccgctc ttgggggaac tggttccgca cgacgagctc
     6301 gggcggtttt tcggcctctt ctctatctcc gggcgcgccg ccgccgtcgc cggaccgctc
     6361 atctggacca gctcggtcta cctcttccgc gccgaccgtc cgctcggccg ctttgcctcg
     6421 gacctcctcg cattgtcgcc atcgggacag gccaaattgc cgtacaaggt ggcagtactg
     6481 tcgctggtgg tgatgatggc ggtcggattg atcatctttc gcaaagtccc tcgtacggaa
     6541 cggtcccgcc atggataagc gcaaggtcgg cgactggtat cactacaccg gcgccgtgca
     6601 catccacacg accgaatcgg acggtaccct cccgctcgag gaggtgatcg ccctcggccg
     6661 cgaggtcggg ctcgatttca tgatgttcgc cgaccacatg actctctcca accggcagcg
     6721 cgggggagag gggatgtacg gcgacacgct cgtcgtgatc ggctacgaac acaacgacga
     6781 gagggatcgc caccactacc tgctgttcgg gtcccccggc gtgtatccgc cctcgctctc
     6841 tgcgcgccag tacgtggcgg cgggagcggc cgacggggcg atcggcatca tggcgcatcc
     6901 cgacgagatc cgcaactcca tgcccgaatt tcccccctac ccgtgggacg actggtcggt
     6961 cgagggtttc accggcatcg agctctggaa ccagatgtcc gaatggatgg agcggctcac
     7021 gccgcgcaac cgcctggcca tggccttcgc cccgcgccgg tcgatgatcg gcccctcgga
     7081 ccgcatcctc cgccgctggg atgagatcag ccggaagcgc aagtgtgtcg gtatcgtcgg
     7141 cgccgacgcc cacgccttcc cgatcaaagt cgggcccttc cggttcgcga tcttcccgta
     7201 caaggtccat ttcaagtgcc tccgcatgac gatcattctg ccggaaccga tgtcccgcga
     7261 cttcgtcgcc gcccgggatc agctctacga agccatccgc gactgccgcg ccttcggcgc
     7321 caacgtccgc tggggtcagg ccgaccggtt cctcttctgc gccgacaacg gccgccggcg
     7381 cgtgccctcc ggggggacga tcgagttgac cgagacggcg cacatgcgga tcgagctgcc
     7441 cgcgcgggcc aggatccggc tcgtgcacga cggctgcacg accgtggaaa acgagggtca
     7501 ggaactgatc caccggctcg acgctcccgg catttaccgg gtggaagcct ggaagggcag
     7561 gcgcggctgg atcttctcca atcacatcag ggtggaagca tgagcaagtg ggaggccgac
     7621 cgggtgaaag tagtcgacct ggccggcacg gaagcgccgg acagccggac cgtgatccgg
     7681 tgcaaattcg tactcatcga gttggcggac gaaattcatc tggttttcgg ccgtttagct
     7741 gactacccgt accacgctcg gttggtcgcg cagttctgca gcgaacgccg catcccctca
     7801 ggctgggaac acgagcccga tctgtatgcg ttgtacacag ctaacgcccg ggtttgcggc
     7861 ggcggatatc tcgctatcga ccctgcctgc cgcacagcgg aagtgtatgg ccggtcaaat
     7921 gcgtacgggc catacgataa gaaagcgatg cacgcggtcg tggaacacgg cccggttttc
     7981 cggggtttaa cggtgtcggt gcggtgagga cggttatgct aatcttttca caaccgacgg
     8041 gtcggaggcc cccgtgccca tgaatattaa gtttattatt agtaacgagt tgtgtctcgc
     8101 tcggggaaac ggggtaactt tcggtgagta ttttaggaaa cggcaaatgg acttgacaac
     8161 ggttggcgta caggatactg ttcgggacgg acttggagaa cgctgagagt tatgtttgac
     8221 accttcttcc cgattctgct cttggttgtt tttgcggtcg gcatcgccgc cgtgctggtc
     8281 gccctgtcag tgatttttgg gcgccggacg gttttgggcc ggaagaccga cccctacgag
     8341 tgcggggtgg agccggtcgg gacgaccagg aacccggtgc cggtcaagtt ctatctgatc
     8401 gccatctcct tcattctgtt cgatatcgag ctgattttcc tgctcccctg ggcggtcgtc
     8461 gcccgcgacc ttgggtcata cggattctgg gcgatggcgg tgttcgtggc gatcatcgtg
     8521 gtgggctacc tgtatgaact gaggcgggga gcgctgaagt gggactagaa gagaaaatcc
     8581 ccgattcgat aatcctgacg acgctggaca agatggtcaa ctgggcgcgc aagcgctcga
     8641 tgtggccgct cgggttcggg ctggcgtgtt gcgcgattga gatgatctcg acctttgcgt
     8701 cgcacaacga cctcgcccgg ttcgggatgg aggtcacgcg gccctcgccg cgccaggccg
     8761 acctcatgat cgtagccgga cgcgtgtcca acaaaatggc cccggtggtc cggaccctct
     8821 acgagcagat gcccaacccg aagtgggtga tctccatggg cgcctgcgcc tcgtgcggcg
     8881 gcgtgttcaa caactacgcg attctccagg gagtcgaccg catcatcccg gttgacatct
     8941 atgtccccgg ctgcccgccg cggccggaac agctcatgga cggcatcctc aagctgttcg
     9001 agaaaatcga aaaggaatcc ctcaaagacg cccgggaaga gtggggccgc agggcggtct
     9061 gaccgcaggc gacaggcaac ggcgatggcg caaacacacg aaccggcgat tgtgaaaagt
     9121 gaaacaagcg agggaaacga cgcccgaatc cgccgcttcc tccgggagcg cttcggcgag
     9181 gccatccgcc acgaggaggt gttccgcggc gatctgcagt tcacggtcga tccggaaaag
     9241 atctacgaca tctgcgaagc cctcatggag gcgggcggac tcgatgtccg cttcctcgcc
     9301 gacatcacct gcgtcgactg gcacggccac tccgaggccg tccacggccg gttcgaggtc
     9361 gtctacaacc tctgctcatt ggcccaccgc agccgcttct tcctgaaagt gcgtctcccc
     9421 gagacgaacc cgcacgtgcg gtcgctcgtc gacctctggc acggcgccaa ctggatggag
     9481 cgcgaggtgt gggacctgtt cggggtgacg ttcgacggcc acccggacct gacgcggctg
     9541 ctgacgcccg acgatttcga ggggcacccg ctgcgcaagg actacccgct ggtctacgag
     9601 cagccgaaat tcagctggaa cgagaacgac ccgcccgagg tgatcaggta atggcggaag
     9661 cccggaccgt gaccatcaac atggggccgc agcacccctc gacccacggc gtgctgcgcg
     9721 tcatcctgga gctggacggg gagacggttg tcaaggcccg gcccgtggtc ggatatctcc
     9781 acaccgggat cgagaagacg atggagtcga agctgtacta caaggcgctc ccgtgcaccg
     9841 accggatgga ctacctcgcc ccgatgtcga acaacctcgg ctacagcctc gcggtcgaaa
     9901 agctcctggg gatcgaggtc cccgagaaag tcaagtgggc gcgcgtgatt ctggccgaac
     9961 tcacgcggat caactcgcac ctcgtctggc tgggcaccca cgccctcgac ctcggcgcca
    10021 tgtcgatgct cctgtatgcc ttccgcgaac gcgagctgat cctcgacgtg tacgaggcct
    10081 gcggcggcca gcgcatgatg accagctaca tccgcatcgg cggccttgcc cacgacctgc
    10141 ccaaggattt cgaccgcaaa gtccgcgata tcatcgccac cctcacggcg cgcctggagg
    10201 actacgaggg cctgctcacc cgcaacgaga tcttcatcaa ccgcaccaag ggggtggcgg
    10261 tcatttcggc cgaggacgcc ctcaactggt cgctctcggg tccgatgctg cgcggcagcg
    10321 gcgtcgcccg cgacctgcgc aaggacgagc cctactccgg ctacgagaat ttcgatttcc
    10381 agatcgccct ggggcataac ggcgacgcct acgaccgcta cctgctccgc atggaagaaa
    10441 tccggcagtc gctccgtatc tgccgccagg ggatcgacgg cctccccgag ggtccctacc
    10501 gggcccacgt cccgggcgtc gtgctcccgc ccaaggagga cgtcctctac aagatggaat
    10561 cgatgatctt tcacttcaag atcatcaccg agggtttcgc ggctcccctc ggctccgtgt
    10621 accaggcaat cgagtcgccc aagggcgagc tcggcttcta cgtggtcggc gacgggacga
    10681 cgatgccccg ccggatccgc gtccggccgc cctcgctcat caatctcgcc agccttcccc
    10741 gactggtcga gggccggctg ttttccgatg tcatctgcgc catcggctcg atcgacatcg
    10801 tgctcggaga ggtggaccga tgattctgtc ggacgagtca gtcagaaaga taaaggagaa
    10861 agtgcccctc tacccgcgcc ggaaatcgtc cgtgctcccc gcgctgacga tcgcctacca
    10921 gcagctcggg tacgtcgacg acgacatcta ccgggagatc gcccgcgtca tcggcgtgcc
    10981 ctatgtcgag gtggccgagg ccgcgtcatt ctatactatg tttccgaaag cgccgcgcgg
    11041 gaagtacctg atccaggtgt gccacaacct ctcctgcgcc ctgctcggcg ccgactcgct
    11101 gatcgcctac ctcgagaaga gcctcgggat caggaaaggc gagacgacgc cggacaagct
    11161 gttcacgctc atctcggtcg agtgtctcgg gtcgtgcgca accgcgccca tgatgcagat
    11221 caacaacgac ttctatgaga atctgacgcc tgcgaaggtc gatcagatcc tgaatgattt
    11281 gaggtcccag gcctgatgtt ctattcgccg gtcatcacca acgcggccga agccgccgac
    11341 aacaagaagc tccacttgtt ccggtacgtc gaggatcctg agcagtacaa gctggcggcc
    11401 tacgagcggc acggcggcta ccgggcctgg cggaaagtcc tcgccggcat gaagccggac
    11461 gaggtgatcg aagtggtcaa gaagtccggg ctgcgcgggc ggggcggcgc cggcttcccc
    11521 accggcatga agtggggatt catccccaag gactccccca aaccgcgcta cctctgctgc
    11581 aacgccgacg agtccgagcc gggcacctgc aaggaccgcg tgctcatgga gcgcgacccc
    11641 cacgccatca ttgaaggcat ggcgatcgcc gcctacgcca tcggctgcca catcatgttc
    11701 atctatatcc ggggggaatt cggccacccg atcgcgcgca tggaagcggc gatgaaggag
    11761 gcctacgcgg ccggccacct cggccggaat atccacggct ccggctacga cctcgagatg
    11821 gtcgtgcata cgggcggcgg cgcctacatc tgcggcgaag aaaccgccct gctgaactcg
    11881 ctcgaagggg agcgcggcat gtcgcgcatc cggccgccgt tcccggccat cgaggggctc
    11941 tacgcctgcc cgaccatcgt gaacaacgtc gagtcgctcg ccgccgtccc gcacatcatc
    12001 gccaacggcc cggagtggta ccgatcgatg gggaccgaga agtcggccgg cacgaagatt
    12061 ttctcgctct cgggccatgt caaccgtccc ggcaattacg aggtcgagct cggatttccg
    12121 ctgaagaaac tgattttcga cccggcctac ggagggggcc tgcgggacgg ccggccgctc
    12181 aagggcgtca tccccggcgg ttcctcgacg cccttcctga cgcccgacgc ggtggagacg
    12241 gtgaacctcg actacgaatc gctccaggcg gccgggtcca tgctcggctc cggcgcggtc
    12301 atcgttttca acgaagacac ctgcatcgtc tggctgatcc tccggctgat ccatttctac
    12361 cgccacgagt cctgcggcaa gtgcacgccc tgccgcgagg ggaccgcctg gctcgaacag
    12421 gtgatccggc ggatcgagca gggccagggg aaaccgggcg acctcgaact gatcgaagac
    12481 gtgtgcggca atatcctcgg gcggacgatt tgcccgctcg gcgacgccgc ggtgatgccg
    12541 attcagtcgg ccgtcaagca ttggcgggac gagtggcgct accacatcga caacggccgg
    12601 tgtctggtaa aatcggaatt cgcattcaag tagaatacca tggctcagga cgtcaaagat
    12661 acgcagccgc agaagaaggc cgagatcccc acggtcacgc tcacgatcga cgggcgcccg
    12721 gtcacggtcc cccgggggac caccgtgctc gaagccgccc ggcagatggg gattcacatc
    12781 cccacgttct gctggcaccc caagctgaaa ccggtgggcg cctgccgcat ctgctatgtc
    12841 gagatcgaga agttcccgaa actgatggtc tcctgcgcga ccgaggcgac cgagggaatg
    12901 atcgtccaca ccgactccga cctggtcaaa cagggacgga aagcggtgct ggagttcatc
    12961 ctgctcaatc acccgctcga ctgcccgacc tgcgacaagg gcggcgagtg cgatctgcag
    13021 aacctcacct tccggcacgg ctacgacgac agccgctttg agttccagaa ataccgcttc
    13081 acctccggcg ccgagggttc gacgttcgac gacaagcgga tcggaccgga aattatcctc
    13141 aatcggaacc gctgtatcct ctgcttcaag tgtgtccgcg ccaacaagga ggcgttcggg
    13201 gaatacgacc tgggcgcgta cgagcggggc tacatcaccg aaatcgacgc cgcgccgggc
    13261 gaacaggtgg acaacccctt ctcgggcaac ctcgtcgaga tctgcccggt gggcgcgctg
    13321 accaacaccg actggcgcta caaaatccgc gtctggttga cccagacgac gccctcgatc
    13381 tgcaacttct ccgccaccgg cgccaacctg ctgttctaca aggaagacca caagaaccgc
    13441 atctaccggg tgacctcgcg gcggaacgac ggcgtggacg acggctggct ggccgacatc
    13501 acccgctacg gctaccagat cgcgcactcc gaagaacggc tgcacacacc gctggtgcgc
    13561 agggacggca agcaggtgcc ggcgtcgtgg gaggaagccc tcgaggtcat ccgcaaacgg
    13621 ttcggcgaaa tcaccgagac caaggggcgg gtgtgcatcg gcgggctggc tgcgccgcac
    13681 ctcgacaacg ccgccctcta tgcgttttcg aagctcttcc gcaccgtcct caagtcgaac
    13741 aatgtcgact accgctccga ctaccgcatg ctcccctcgg ccgccgacag cccgttcaac
    13801 ctcctctgcc ggcgcccctt ccgcatcgcg gacatcgacg cgagcgacgt catcgtgacg
    13861 ctcggctccg acctgctccg cgagcaccag ctcgaatacg tgcgtattcg caaggcctgc
    13921 actttccgcg gcgcccgcta tttctcgatg accccctacg ccgtgcggtc ggccgacatc
    13981 gccgaggccg aactggtcta cgcggtcggc atggaagagg tgttgctcac ggcgctcgga
    14041 ctggcggccg tggacatggg gctggtcgat ccctccgccg gagccgtgtt ccgggcgcgg
    14101 gtggagccga aaacgctcgc cgaggcgtgc cggctgtgcg gggtggcggc cgagaccgtg
    14161 aaactggcgg cgcgcgccct ggccaccgcg ggccgcgtca ccttcttcac cggcgaaatc
    14221 gtcacccggt cctacgcccg cgatgcgatc ggggcggccg ccctcaatct cgaccggctc
    14281 ctcggcctgc accggcgcgg ccagatcgcc gcgcttgccc ggtacgccaa ctccgccggc
    14341 gctgaacggc tcggcgtgtc tccgacgccg gccccggccg tggcgaaaag gctcgccgag
    14401 ctctgggggg aattccccga ggccgagccg ggcaccaccg accgcatgat ggtccagatg
    14461 cggcgcgacg aaatcgacgg attcttcatc ctcggcgcca acccggtcat gctctacccc
    14521 gaccggcagt tcgccctcga gtccctcgag aagctcgatt tcctcgtggc ggccgatcta
    14581 ttcgagacgc ccacgaccga gctggccgac gttgtgctgc cgctggcgag ctgggccgag
    14641 ttcaccggcg actatgtcaa tctcgagggc cgggtgcagc gggcggagcg ggcgatcaag
    14701 ccgctccacc aggcgaaacc gcccctggag atcgtccggc tggtcgccga caagctcggc
    14761 gggaagctgt ttgcgagcga ggagcagatg caggccgaga tcgacgcggt gctggcggtc
    14821 gacgccacgg ccgcaccctc cggcgaattc ttcgaggtca agccggctgc ggagccggcc
    14881 tccgaggagt acccgctacc gctcttcatg ggcgatgatc cccaccacag cggccacctg
    14941 acggagaaat ccgtcgcgct ggcgaacttc tgcagcgagg cctaccttga aatgtcgccg
    15001 gaactggccc ggcggtacgg cctcgacgag ggcgacccgg tgcggatcga atcggagctc
    15061 ggcaaggtgg tcgcgcccgc gcggatttcc gacgttctcg acagcgatgt cctgctcgcg
    15121 ccgcgcaact tcgcctcgac cccggtcaac agcctgctga cgcgcaaacg gcggatcgac
    15181 cgcgtgaaaa tctccaaggt gattgactag acgatatgct ggaagccatc atcatagcgg
    15241 cggtgaaagt tctcgcagtt ctggtcgcgg tgctgaccgg atgcgcctac gccacttaca
    15301 tggagcgcaa ggtgctcgga cacctccagc accgcatcgg cccgtcctac gccggtccct
    15361 acggcctgtt gcagccgctg gccgacgccg tcaaactcat cttcaaagag gacgtcgtgc
    15421 cggccaacgc cgagcgggtg acctacacgc tggccccgat cgcctcgttc gtccccgcga
    15481 tgctgacttt cgccgtcatt ccgttcaccg ccgatttcga gctgttcggc cgcccgatcc
    15541 agggcgtcat ctccgatctc aacataggca tcctgtgggt cttcgcggtg acctccctcg
    15601 gcgtgtacgg tatcgtgctc gccgggtggt cctcgggctc caagtactcg ctcctcggag
    15661 gcgtccggtc ctcggcccag atgatctcct acgaaatcag ctacggcctg accattgtcg
    15721 gggtaatcct gatcgccaac accctctcga tgcgcgagtt ggtggagcag caggcgtcga
    15781 tcgccgactg gttcatctgg aagcagccgc tcgggtttct cctctacctg acctgcgcgg
    15841 tggctgagac caaccgcgcc ccgttcgacc tccccgaggc cgagagcgag ctcgtggcgg
    15901 gataccacac agagtactcg tcgatgcgct ttgccacctt cttcatcagc gagtacgggg
    15961 ccatgatcgc cgtctcctgc gtggggacga cgatcttctt cggaggctgg caggggccgt
    16021 ttctgccgcc gttcctctgg ttctttttga aagtgctggc gttcatgttc ctctatattt
    16081 ggctgcgggc gaccttcccc cggttccgct atgaccagct catggcgttc gggtggaaag
    16141 tgctgttccc gctggcgctg ctcaactcgc tggtgacggc ggcgtttgtg atgctctaag
    16201 gagagaggtc gcatgccatg aaggacgtac tgactgcctt gaccaacgcc gtgatcaaga
    16261 ccccgaaggg cctgctgatc acgctgaaac atctgttcaa gaagccggtg acggtcgatt
    16321 atccccggca gaagcggccg atggcgccgc gctaccgcgg ccgccactac ctcgaacggt
    16381 atgacgacgg caccgagcgg tgcgtctgct gcggtctgtg cgccgccgcc tgtccggccg
    16441 acgccatcta catggaaccg gaggagaacg agaaggggga gcggcgcgcc cgcacttacg
    16501 aaatcaacct cctccgctgc atcttctgcg gctactgcga ggaagcctgc cccgaggaag
    16561 cgatcttcct cggccaggac tatgaattct ccagcagcga caagagttca tttatctgga
    16621 agaaagacga catgctcgtc gcgcacccgc gcagagacaa ccccttcaag agactctacc
    16681 ggcgggtgcg caaggtgtac ggggtggcgg acaaggtgcg atgagtctcg acgcaatcat
    16741 attcgtgctc tcggcggtgg tggccgtgtt cggcgccacc atgatgatcg tccagcgcaa
    16801 cccggtggcc tcggtgctct acctgatcgt ctcgctcatc gcccaggctg tcctgtacat
    16861 ccagctcgga acggccttca tgggcgccgt cctcgtgatc gtctatgccg gcgctatcct
    16921 cgttttgttc ctcttcgtca tcatgctcct caatctccgc gggcgggagg atctcggcga
    16981 ggggtcggcc ccgctcagcc ggctgaccaa gtacctcgtc tcgctgctct tcgtggtcga
    17041 actggtgttt gtcatccggg cgggattctt cgccgaaacc gccgtcgtcg gcgtcttcgc
    17101 cggcggggcg gttgccgacg atttcggttc ggtggagcac acggccggcc tcctgttcac
    17161 gcgctacctg tatcccttcg aattgacggc ggcgctgctg ttggcggcgg tcgtcggagc
    17221 ggtggcgatc gccaagcgcg accgggcgga ggggccgccg gccggcgccg agacgcgcgg
    17281 ccgcgccgag gacgccccgc cgcaaacgca caaggtggag tcctagcatg gttccggttt
    17341 ccgcatacct gattgtcgcg gccgtgctct tcgggatcgg cacgatcggc gtgctcgcct
    17401 cccgcaacat gattatcctg atcatgagca tcgagctcat gctcaacgcg gccaacctcg
    17461 ccctgatcgc cttctcgcgc gcgctcaatg tcatggacgg ccacgcgttc gtgttcctcg
    17521 tgctgaccgt ggccgcggcc gaggcggccg tgggactggc catggtgctc accgtgttcc
    17581 gcaaccggga ctcgataaac atcgaccggt tcaacctgtt gaaatggtag ggccggggac
    17641 gctatgtcgg attacctgtt tctcataccg cttttgccgc tgatcggctt cgtcgtcaac
    17701 gggctgctcc tgggccgact ccgccgcccc gccgtcaacg ccgtcgcctg cgcgtcggtg
    17761 ggcggagcct tcatcctctc ggtgttggct ttcttcgagc tgcgggcgct gccgccggcg
    17821 atgcggatga tcgagcaaac cctcttcacc tggatcccct ccggcaattt caacgtcaac
    17881 atcggcttcc tgctcgaccc gctctccgcc gtgatgatcc tcgtggtgac cggcgtggga
    17941 ttcctcatcc acgtctacgc gaccggctac atggcccacg acccggggtt cggacggttc
    18001 ttcgcctacc tcaacctgtt catgttcgcg atgctcacgc tcgtgctcgc cgacaacttc
    18061 ctgctcatgt tcgtcggctg ggagggggtc ggcctctgct cctacctgct tatcggtttc
    18121 tggtacgaac ggcagtcggc ggcggacgcg gggaagaagg cattcctcgt caaccgcatc
    18181 ggcgacttcg gtttcctcct ggggatgttc ctcattttct ggcaggtcgg gtcgctccat
    18241 ttccccaccg tgagtgaggc ggcgcccgcg gtgttcaccg ccggaggcgg cctcatcacc
    18301 gccgtctgcc tcctgctctt ccttggcgcg accgggaaat cggcgcagat cccgctctat
    18361 gtctggctgc ccgacgccat ggagggcccg acgccggtgt cggcgctcat tcacgccgcc
    18421 acgatggtga ccgcgggcgt ctacatgatc gcgcggacga atgtgctgta cagcctcgcc
    18481 tccgacgccc tgctcgtggt ggcggtcatc ggcgccgcga ccgccttcgt cgccgcgacc
    18541 atcggcctgg cccagaacga catcaagcgc gtcctcgcct actcgacggt cagccagctc
    18601 ggctacatgt tcctcgcctg cggcgtagcc tcgttcacgg ccggcatctt ccacctgatg
    18661 acccatgcct tcttcaaagc cctgctcttc ctcggcgccg gttcggtcat ccatgcgatg
    18721 agcgacaaac aggacatgcg cgagatgggc ggactgcgga aacatctgcc ggtgacctac
    18781 gccaccatgc tcgtcgccac cctggcgatc gccggcatcc ccggcttctc aggattcttc
    18841 agcaaggacg agatcctctg gaaatcgttc tcgtcggccc acggccaccc gctgttctgg
    18901 gtgatcggct tcgccactgc cggtctcacc gccttttaca tgttccgcct ggtcttcctg
    18961 accttcttcg gaaaagagcg gatggaccag cacacgaagg accacctcca cgagttcccc
    19021 cggtcgatga cggttccgct ggtgatcctt gcggtgctgt cggtggtcgg cggatatgtc
    19081 ggcatcccgc acgtcttcgg cgcgaccaac tggttcgagc gctggctgga accggtgatg
    19141 gccggcggcg ggggggaagc ggcgccggag acggtgcacg cctcggccgg cggctacggg
    19201 accgggatgg agcttgccct catgggcgcc tccgtggccc tcgtgctcct ggccatctat
    19261 ggcgcgcacc tgttctaccg caagcggacg gagctggcca cccgctggcg ggagcgcctc
    19321 tccggtctgc accgcctcct ggtcaacaag tactacgtcg atgagatcta tttggccgtg
    19381 atcgtccggc ccctggtcgg gttatcgatg ttcctgtgga agttcgtgga tgtgatcgtg
    19441 atcgacggac tcgccaacgg cagcgctgcg gcctaccagg gcctgtccga gggtgttcgc
    19501 cgcctccaga ccggccgcgt ccggagctac gccacgctct tcgtgctcgg cgtcgtcctc
    19561 ctggtcgcct actttgcaat ggattgggga tggaaaatca aattctgacc tacgtcacat
    19621 tcttcccgct cgtcgggatt gtcgctctcc tgctggcgcc gaaggaacgg cacgacacga
    19681 tcaaggcggt cgcgctcatc gtggccgtca tcaccatgct cctgtcgttc ttcctctggt
    19741 caatgttcga cccgatggcg ctggggatgc agttcgaagt cgacatcccg tggatcaccg
    19801 ggctgggcat ccactaccac ctcgggatcg acggcatctc gctgctgctg atcgcgctga
    19861 cgaccgtcct gaccgttctg gccatcctcg cctcgtggaa ctcgatcacc aagggggtga
    19921 aggggtacta tctctcgatg ctcctgctgc aggtggggat gatcggcgtg ttcgtgtcac
    19981 tggatctctt cctgttctac gtgttctggg agatcatgct ggtcccgatg tatttcatca
    20041 tcggtctgtg gggcggcccc cggcgggtgt acgccgcgat caagttcgtc ctcttcacca
    20101 tgttcggctc gctcctcatg ctcgtcggca tcctctggct gctcttcgcc taccacggct
    20161 actccgggga gtattcgttt gacatgctca agctcgtgga gatgccgatc ggctaccggg
    20221 cgcagctctt gttgttcggg gccttcgcgc tggccttcgc aatcaaggtc ccgctctttc
    20281 ccttccacac ctggctgccc gatgcccacg tggaggcgcc gacggccggc tcggtcatcc
    20341 tcgcgggcgt gctgctgaag atggggacgt acggtttcgt gcgcatctgc ctgccgatgt
    20401 ttccggacgc caccgtggcc tacctcccct acatcgccgt gctgtcggtc atcgcgatca
    20461 tctacggcgc gctcgtggcg atggtgcagc gggacgtgaa atcgctcgtg gcgttctcct
    20521 cggtctccca catgggcttc atcatgctgg ggatcttcgc cctcaacctg cagggactcc
    20581 agggcgcgat catgcagatg gtcaaccacg gcgtttccac gggcgccctg ttcctcctgg
    20641 tggggatgat ctacgaccgc cgccacacgc gcctcattga ggaattcggc gggctggcca
    20701 aagtcatgcc ggcctactcg gccatcttca tgatcgtcgc cctctcctcg atcggcctgc
    20761 cgttcaccaa cgggttcgtc ggcgagttcc tcatactcct cggcgcgttc gaccgcagct
    20821 atgtctacgg caccctggcg gcctcgggcg tgatcttcgc cgccgcctat atgctctgga
    20881 tgtaccagcg cgtcatcttc ggaaaagtca ccaacccggc caacgcgcaa ctggccgatg
    20941 tcgactggcg ggagaagatc atcctgatcc cgctggtcat cttcatattc tggatcggca
    21001 tttacccgaa acccttcttc gagcgcatcg aacccgccgc ccagcaggtt ctcacgcggg
    21061 tcgggcgcgc gcagacggtg cagctcgacg accagttccg ggtaaacctg gtcccggccg
    21121 tcgatatctc cccgcgcggc gacacgatta cggtgggact gggggccccg gagagaggta
    21181 cggaatgaac aacgtggtca tgtacaccaa ggaaggctgc ccgtactgcg cggcggccag
    21241 gaagcactac gcggagcagg ggatccgctt cacggaaatc gacatccatc gcactcccgg
    21301 ggcccgggac aaggtgcttg ccctgaccgg cggcgaggct atcgtcccgg tcatcgtcga
    21361 caacggacag gtcatcaaag gcttcggcgg cggctgaggc ttctaacggt gcgggcggcc
    21421 gggcgccgcc ctcggaaatg ctatgataga tctcacatcg gccggcattg acttcggcct
    21481 gatcggaccg gaaacggcgc tcctcattgc ggcgtcggtc attctcctga ccggctatct
    21541 cggccgcctc ggccgcttca gccccggcct cagcctggtc ggcctgtccg tggcgctggc
    21601 cctggcggcg gcttcctggg gccggcaggg atcgggcttc tggggcatgg tgacgaacga
    21661 caacttcggc tcggccttca aagtcatcgc cgccggcacc tcgatcctcg cccagctcat
    21721 ggcgcagcgc tggctggcgg tcaaggggta ccaccggccc gaattctacg ccctcctttt
    21781 gctggcgacc atggggatga tggtgatggc caataccacc gacctggtcg tgctcttcct
    21841 cggcctcgag ttgatgtcgg tgccgctcta tgtgatggcc ggcttcgcca agcgctcgct
    21901 cgagtccaac gaggcgggtc tcaagtattt catcatgggc gggttcgcca gcgcgttcct
    21961 gctcatgggc attgccttcg tgttcggggc ggccgggacg accgacctcc gccgcatcgt
    22021 cgccgacttc aacttcatcc tgtcccgctc ggatgcctac atgtacgcgg gggcggctct
    22081 catcctgatc gggttcggct tcaaagtcgc cgccgtgccc ttccactcct gggtgcccga
    22141 cgtgtaccag ggggcgccga ccccggtgac cgcgttcttc tcggtcgggc cgaaagccgc
    22201 cggcttcgcc gcgctcctgc gcgtgttcgt cttcgggttc gcctcgatgg aggccctcac
    22261 cgacgccttc tgggtcctcg cggtgctgac catgacggtc ggcaacgtcc tcgcgcttcg
    22321 ccagtccaac atcaagcgcc tgctggcgta ctcctcgatc gcccacgcgg
//
LOCUS       DAOPOE010000051        21380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3056_length_21380_cov_5.153669, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000051 DAOPOE010000000
VERSION     DAOPOE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21380
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_21380_cov_5.153669"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1793
                     /locus_tag="PKM94_08165"
     CDS             153..1793
                     /locus_tag="PKM94_08165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD66652.1"
                     /translation="MAKLIGTDGLKVYAFDLPPGSYVVGRNADCGICIPDRTVSRRHA
                     ELEVEAAGANIWVADLGSHNGTSVNGLRISARTAVKPGDMIIFGQVEFRLTDDPDAGV
                     RSNLTRLSAVEPERSVYLDISEALKPLPARVTDRPEVVPTIFEMAKLLVLPEPEQVLL
                     ERSLGLIARLIPADRLAVLVRGEGDELYTAATLLPEGKDMGEFRLSSTIVREILTDKQ
                     SIVVGNPMEDPRFAQQQSIILSEMKSAMAVPLFDEGNVHGILYVDTTNPLHRYSDEYL
                     RLLATFGNILGARMLSYTLQRERQEKHVMEAELARAASIQKNLLVQAYPTPGGYDLHA
                     FQEQSRAVGGDLYDIAALPDEHVLFLVADVSGKGMGAALLMSNILASFRILYDQADFD
                     LCRMVRLVSTQMYRYSAPENFATLFVGVIDAATHEIRFVNAGHNPPIVVRADGRHEHL
                     PASGIMIGAMPFDAWSEERVTLQPGESLVVFTDGVTEAERADGSLFGEDRLEAVLTGA
                     RGQAPAHLCETIMKEIRRFVENAPQSDDITMLVVQRKA"
     gene            1793..4939
                     /locus_tag="PKM94_08170"
     CDS             1793..4939
                     /locus_tag="PKM94_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOD66653.1"
                     /translation="MLQPGQEFAHFRIMRELGAGGMGAVYLAEDLKLHREVALKIILA
                     EYFNDAERLGRFQREAKMAARIAHPNVMGIYDIGSAPDPDTGRDLEYIVMERVKGIPL
                     GEYFQSAKPDMGGVIRVASRIAAGLAAAHKLNIAHRDIKASNILVDDEGNPKILDFGL
                     AKPVDPVQFDEGDRTDTVSQELTRAGKIVGTVSYMSPEQARGDAVDIRTDIFSFGVLL
                     YRMATGEFPFAGQSQVTTLAKILESKHEPPRLKNQQIPTELERIIDKCLQKDPNDRYP
                     SAADLTVDLRNLRRQFDSGVTESISGEYRVERTVRVGGKRLAAIAAGLVVVGIVAVIV
                     WRSGGERPGGAGPGGISPSVTTPGGNAVAVFDFENKTGDTSLNWLQTGLPEILMTDLA
                     QTGSITVISRERVLDHLRTGSDLPDADEISDDVRAEIEQARDEIREAVKSGSLDGVPA
                     VKDALELARVSVAGLMRPHTYAEMAQAAGALGASTVLSGSFFKLGERIRIDARLEDIG
                     SGKIVLAEKVMGNDPFALVDSLTGKIVASLNVKDQMADQSVANVITGSPEAYRLYREG
                     MDLFGLGMNDEAIAKFEAAAGVDSAFALAYMRIAMVHVFEGRLQKGMQYLARADAFKE
                     SLPVRERSLLDIYTDLWLEQELDAAFVKLRAYVENYPSDKEARVLFGLATWVFNQDTA
                     AARAQYDTVLRADPVNLRALAWTGDLLRQSGQLDSAVYYYRKVKAYHPDSPEGYTNLG
                     ALYREQGDIPGAAQEYLLMSEAFPRLAAPWMNLSTLAIRQRDFEGAGRFLDEYRGRTG
                     KDPYGLYEYEMQLSGLEQWRGQFRSARDHMRAALDYARQTKDSNYVRSAYNTLFSFYR
                     RLHMDDSALWCARQTEPWSTGIARLNVAMNYAEAAPADCGRARAFLEEGTAAFKQRVP
                     SGLWPVAEAVGEAFDGYCAGDTAAVIAALLKLSSAQQPGQGEGNRREAGVLQVLTGHY
                     REGRDILVRSLSGKDEVASGATYPYFLYLVGVAEQELGDTKSAIARFEEMLRYWGKPD
                     IETKEIADARRRLAALRS"
     gene            5059..6300
                     /locus_tag="PKM94_08175"
     CDS             5059..6300
                     /locus_tag="PKM94_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD66654.1"
                     /translation="MIARSARYLRQFDLNLWILSFGWFNSSMGFSISLPFISIYFYSE
                     FGMSMTEIGVFFGALAFVRSFFQAVGGEASDRIQRRWLLVYSQYARAVSFLAMAASIH
                     FHLGFWATAAALAVNSILGAIFQPAANATVADILPKAQHLDGYAITRSAGNLGWAAGP
                     AIGGFLAGWSYAALFAVSALMALLSGLVFHLLLRVPEATRAVDRFRLRDILTIRDDHH
                     LLRHAALLFLLYLVVAQLTVPFSVYSVEMAGISKVQLGTLYTINGLLVVALQIPVTRL
                     LSRFTLTAQIAMGSFFYAVGYGSVGILVGYHYFALAMVVVTIGEVIMSPPTLTLTSRL
                     APEGRMGRYMGVHGFVVASGWSFGPLYGGVILDHFPHTWSVAWMMIASLALVSGAGYL
                     WFRRCLPEAVNKARYAPPVTE"
     gene            complement(6290..8920)
                     /locus_tag="PKM94_08180"
     CDS             complement(6290..8920)
                     /locus_tag="PKM94_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66655.1"
                     /translation="MQPPLGSAARCLVFLLILAAAWWPPPAAARERTPKERLAARYGG
                     HQRAEGRTIMYIAHSRGNIQLAVANNGTFGTLGTTIADPITGEAIQACVYPKNTDLVY
                     LWVAAVWIGAVVGRDTLVSVGNEDFYVTEEFSPVDYDWEDLQPPLGFKYRSIDINSSW
                     YHPEALSEEDIICTYADTLDDPAIVESDNTDNRPHKPLGVKITQRSMAWSYAYADDFI
                     LFDYQVENIGQKLIRDIYIGIYVDGDVWHVTRNGPDGWNDDVVGFYRTHPAEGRCGFI
                     DTINVAWHADNDGDPDETSGEWDYRSPRSAVGVRVVRTPAAELAYSYNWWIINYSDPS
                     RDFGPRTRPTDSDPFRNFGRRIGSPEGDRNKYYMLRHEEFDYDLYYTATIGPSNAVWM
                     PPPADQAAEIARGYDSRYLLSFGPFDINPGQKLPVSFAWLGGEDFHTQADAFETLFDP
                     YNPTPFYNYLGFDKLAGNAKWASWVYDNPGVDTDSDGYAGRAHCQASLADGTPVCLYN
                     CDDTVGAVSSGAGWDVQWYVGDEVPDFRGAGPPVAPRIRVIPSDGRLIVRWNGYYSET
                     TEDFFLNAVDFEGYRVYLARDNRPGSFSVLSSYDHENYNRYVFDATRSDWVLQGTPLT
                     LDELRVEYGDSLFDPLRYTPSSSFAWEGSHYYFTRQDNNRSDLTSSRGIHKVYPDSPR
                     PPADRAEWRPDDLTFEHGEGLPKYYEYEYVIDDLLLTVPYYAAVTAFDFGSPVVGLPP
                     LETSPLINAVLEYPQTAAAAVAEDRLDVYVYPNPYRLDGDYRGLGFEGRQDSDRPDER
                     VRKLHFANLPLRCTISIFSLDGDLIKRIEHDRSPGDASSAHEEWNLITRNTQILTTGL
                     YYWVVESEGRTQIGKFAVIR"
     gene            complement(8933..11509)
                     /locus_tag="PKM94_08185"
     CDS             complement(8933..11509)
                     /locus_tag="PKM94_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66656.1"
                     /translation="MRKAVTVSALACLLGAGLAADAWGRAEFIGSEERRGRSQAAQQP
                     EVPLCVASHRVNEIVLAINNNGTFGTGFTLGSTGDCFSGGQVYSCEYPKGSNTQYLFS
                     AAFWVGAVLGRDTVVSLGADGWSSTREMFPFRRPLLRRSIVDPEDSEYEGAVSEEDFV
                     ATYTDTFTAGIPPDLYGQPHIPIKIAVTENSYGWSYSYAEDFILFDYQITSMNSQMLN
                     DVYLGIYVDADVYHPTTTNGYSDDLTGYLEKYSQVIGPCSYADTINIAWLADNDGDLD
                     FPRPVPHVTGMRIVRTPADTLEISFNWWFSASGELDFGPRERPYTGKWKEPFRDFGTG
                     GTGTPEGDRNKYYIMSNQELDYDQARVASIGPTDPLWVYPKQELVAEWADGADTRYLL
                     SFGPFNIRPGQTLPLSFAYVGGKNFHTYRQNLSNLPYNFDAFYDSLHFEDFAENARWA
                     GWIYDNPGVDTDSNGFAGRAHCQTRLSDGTAICLYNCEDTTGADAAGAEWDIHWYTGD
                     GVPDFRGAAPPPAPKFQLIPSFGKVRVRFNGTYSETTEDPFVGLVDFEGYRIYIGRDD
                     RKTSFSVVCGYDRENYNSFHWLPDRRTWEISPVPLTREELACRFGDSCRDANFDPLAY
                     SMVEPLRTPAGELYYFTPQDFNRSVLGIPGAIRRAYPDQPKPATDDPRQADPDELAPD
                     GLFKYYEYEYDIEGLLPSVPYWINVTALDYGSPRSGLPSLESAVTQGAQYVIPLSDPE
                     KARSEELPIIVYPNPYRIDAEYRSLGFEGRGQDDRPDHRVRCVRFANLPPRCTIRIFS
                     LDGDLIRTLEHDMPPSDPNAPYEEWDLITRNTQLVVSGLYYWTVEAEGRETQIGKLAV
                     IK"
     gene            complement(11719..14103)
                     /locus_tag="PKM94_08190"
     CDS             complement(11719..14103)
                     /locus_tag="PKM94_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66657.1"
                     /translation="MNKRIALVAACCLSLVLWWSGAASGAADASLKRPVPVKSALKSL
                     RAGDGAGRVVFKLAEAPDRPAVAGGRFVQSGAQWDRLNAIVAGPAKAARELAPHIPLD
                     PAVLDRMRQEASARVGWAMPDLTRYYELRFPASATAAEKLAAVNDLNALDLVEIAFFQ
                     PDPEPASGRSLSPAMLSIAEVQATPNWENNQYYLKAAPIGVNAYYAWNYPGGKGETVR
                     VIDIEGNWIETHEDLHGGTDNFHIAGTRINDAGWWNHGTAVLGEIAADSNSFGMTGIA
                     FNVDLGTVSIGSMSTAAAITTAVANSEAGDVFLIELHAPGPHYDFEPRDDQAGYVPME
                     YWQDVFDAILQASALGRIVVEAGGNGNENLDDVGIYGQTFDPAYRFSGAIMVGATSAG
                     HVPASFTNYGQRVDVHAFGTWDVYTLGYGSLYGTGINDSYTGSFSGTSSASPIITGSA
                     AVLQGINKATHGTTLYHNEIRSLMQTYGTPQAAHYKHVGLLPDLQGATDQVIGVSFSA
                     DTTIGWAPFDVAFTGSSGLEVTSWTWDFGDGDSAFVQDPVHTYEAGGQYTVRLEIMAD
                     GQPRVAQRVDYVAVLADTMVAPAAQGGRGDTVIVPIYARNNVPLTRIDIPVEYPGDIL
                     ISSATFNTVGCRTSYFQVQDLWYSFPGGRRMAVRLQSSVTGTAPPLEPGAGPIVNLVF
                     VISPEAEFPQSADVKLTGYGTYLPAFSGPLATYTPVLQAGTLDLCPPRGDLDGSPGIS
                     VADITYLVGYLFRSGPPPAHLETADVNCDGSVKVSDLTYLINYLFRSGPDPCAC"
     gene            complement(14234..15103)
                     /locus_tag="PKM94_08195"
     CDS             complement(14234..15103)
                     /locus_tag="PKM94_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66658.1"
                     /translation="MAPLSTAADSPRIVFDVQDTTASPGESAAWLDLYLSNYVDTIAG
                     LEFRLSVDRPDLVWFDLSGPDFDTAGALLSGFELVLARDSADHSIYRLIALANLPFSG
                     PETPGIPAQNGAVLARLPFSTAAQPDTTGGLTCNITVTGPYTFSDPRGNSIGMVVVDT
                     VPDTLFYRCTAWDGDICLGWVQIDPDTADWDSIYVYDIYVHEMDTTLVVMGPGSIKLV
                     LPEPLMCDFNGDGAYGIGDLTCLTNLLFRGGSCPSVRCDCDGSGNDPAQPTIGDLTCM
                     VAFLFRGGPPPAD"
     gene            complement(15377..17515)
                     /locus_tag="PKM94_08200"
     CDS             complement(15377..17515)
                     /locus_tag="PKM94_08200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD66659.1"
                     /translation="MKKWTTLLCLWWLAALALPAAAQDVESITFPPLNPLEIPKVEKI
                     QLDNGLRLYLLTDKSLPLFNVNVRLAAGSYLEPGDQAGLAEITGTVMRTGGTRTWTGD
                     QIDELLEGIGGMVETNFGDVEGGAFVNVLSDYADTGLLVLAEVLRYPRFDQDKIDLAM
                     VQARTNIARRNDEVSEIALREFRKLIYGAESPYARTMEYATLDKVTRDDLVAFHDRYV
                     RPENIQMAIYGDFDKNKVLARVKKLFGDWKKGTAAVPPPPVVDYQWRKQVYYAEKKGA
                     KQSYIRMGHLGGMLKDPDYADKIVMNSILGLGFGSRLTDEVRTRLGLAYSAGGRYVSN
                     AAYPGYFFALASTDPGNTVKACRAMIEQIRSMQTVPPTDMEMKKGKDGYLNSFVFNFD
                     SRREVIGRIMSYDFYGLPEDFLQREKTRVEQTTKDDVVAAAKRSLHPDSMVILVVGDK
                     EKFDLPLDSLGWGPIEAVDISIPAPQQADQEELVVNDANIAKGMEILGKAIAAHGGTE
                     AFAGVTSVVSTRSIKLVMGGQELPATVNSWEVIPDKEKSEISIMGQKIISLRVGQQGW
                     RTDQTGQMVPLSADEVAEGEKERLRETIRVFQAYGNPYYKAVYNGRGELGGTPVEWVA
                     ILDASGEQLARFGFDAGSHTLAAKMYFDQTMMGEGMVTEKYSNLQPKGKITLPTVTVT
                     ELNGQKIMEENISVFDVNTAIAAEVFAPPQ"
     gene            complement(17530..19035)
                     /locus_tag="PKM94_08205"
     CDS             complement(17530..19035)
                     /locus_tag="PKM94_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD66660.1"
                     /translation="MRRMILRAGTAIACLALVSAPLVFAFDFQKIEKTVSEHTLANGL
                     KIIVMERHEAPVASFVTYVDVGGVDDPKEYTGLAHMFEHMAFKGTRQVGTQDIDTELQ
                     LMKVEDSIFYELRAERKKGAQADSTRLAALQKAFDEAIEAANRVVVPNEFDNILERHG
                     AVNLNAGTGMDQTSYIMSLPSNKLELWMAMESARFNQPVLREMYREREVIAEERRQTL
                     ENNPIGRAIDVLKSTAFTAHPYGISIVGHMSDIKNYTRDAAKAFFEKYYVPSNMIIAI
                     CGDVYPDEVFRLAEQYWGGMPAKPNPEPVATVEPEQQGERRVILEDPAQPFFAAAWHV
                     PEYTHPDWPAVQALMDYLGQGRTSLLYTNLVKEKKIAAEVFAFAGYPASKYPTLALVG
                     VIPAADHSNEECESEVYVEVERLQNELLPAEDVAEIKARAKADFINGLTSNLGMASQL
                     ASAQNDWGDWRALFKELGRINGVTPEDIQRVAKKYFTKNNRTVVCLNTVQS"
     gene            complement(19231..>21380)
                     /gene="glgP"
                     /locus_tag="PKM94_08210"
     CDS             complement(19231..>21380)
                     /gene="glgP"
                     /locus_tag="PKM94_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOD66661.1"
                     /translation="ASDIGLPMTAVALLYRYGYFRQFLSSDGWQQERYEENDWYHMPV
                     ELVKDANGQPLRITVQMAGAPVHVQVWKTTVGLIPMYALDTNIPENSTKNREITSVLY
                     GGDKDMRIRQEILLGIGGVRALRALGIEPAIFHMNEGHSWFLALERLRILMQEQGLSY
                     NEALQYVWATTVFTTHTPVPAGNEQFDPVLVHRYLGDFLASLGVPWKDFLTLGRVMPE
                     DESEPFGMTVAALKTSAFANGVSKLHGRVSREMWHNIWPGLPIEEVPIKAITNGVHTQ
                     SWISHDMDELYESYLGPRYRERPGAPEVWKRVQKIPDMELWRTHNRRRERLIFFARKR
                     LKQQLQRRGASQLYIQRAEQLLDPQALTICFARRFSTYKRGSLIFADLARLERLVNDE
                     KRPVQLIFSGKAHPLDNPGKEIIKYIVGVISEERFRNRIIFLEDYDINVARYMVQGAD
                     VWLNNPRRPQEASGTSGMKAALNGALHVSVLDGWWAEGYSDERGFKIGNGEEYENVEI
                     QDRLEAEMLYDVLEREVVPLFHNRNGVGLPEKWIQKMKAAVQMAGEEFAAQGMLRNYT
                     DEFYVRAIHSARQLRDNNFAKTRSLTAWLDRMRAGWGTLAINDVRIPDVGATVYVGQK
                     LPIEADVFLGAITPDDVSVELVAGRLNSQEQLVNYRSVPAKLVKPVDGGGTYTFAAEV
                     ECTESGRFGIKARIIPRNDALPHVFKPRLISWW"
BASE COUNT         3575 a         6766 c         7163 g         3876 t
ORIGIN      
        1 tctgaccgcc atggcccagg ccgggtgggg gcagcgcgag atcaacggct ttgcccggat
       61 tgccgccttt ttagttagca aatacggcac gatctaggta tactgcggcc cgaccgggta
      121 tcgaacctgt cgccgacgga gagtttcttc tcatggccaa gttgattggg accgacggac
      181 tgaaagtcta cgcgttcgat ctgccgccgg gcagttacgt ggtgggccga aacgccgact
      241 gcggaatctg tatccccgat cggacggtct cccgccgcca cgccgagctc gaggtggaag
      301 cggccggggc gaacatctgg gtcgccgacc tgggcagcca caacgggacg agcgtcaacg
      361 gcctgcggat ctcggcccgc acggcggtca agccgggcga catgatcatc ttcggccagg
      421 tggaattccg gctgaccgac gacccggatg ccggcgtccg ctccaatctc acgaggctgt
      481 ccgcagtgga accggagcgc tccgtctatc tcgatatcag cgaggccctc aagccgctcc
      541 cggcgcgggt gaccgaccga cccgaggtgg tcccgacgat ttttgagatg gccaaactgc
      601 tcgttctgcc cgagccggag caggtgctcc tggagcgctc gctcgggctg atcgcccggc
      661 tcatcccggc cgaccggctg gcggtgctcg tccgcgggga aggcgacgag ctgtacacgg
      721 cggccacgct cctgccggag gggaaggaca tgggggagtt ccgcctctcc agcacgattg
      781 tgcgggagat cctgaccgac aagcagtcga tcgtggtggg caacccgatg gaggacccgc
      841 gattcgcgca gcagcagtcg atcatcctgt cggagatgaa gtcggccatg gcggtgccgc
      901 tcttcgacga ggggaacgtc cacggaatcc tctacgtcga cacgaccaac ccgctccacc
      961 gttacagcga cgagtacctc cgcctgctag cgacgttcgg gaacatcctc ggggcgcgca
     1021 tgctcagtta cacgctccag cgcgagcggc aggagaagca cgtgatggaa gcggaactgg
     1081 cgcgggcggc ctcgattcag aaaaacctgc tggtgcaggc gtatcccacg cccggtgggt
     1141 acgacctcca tgcctttcag gagcagagcc gggcggtcgg gggggatctc tacgacatcg
     1201 ctgcgctgcc ggacgaacac gtgttgtttc tggtggccga cgtgagcggg aagggcatgg
     1261 gggcggcgct gctcatgtcc aacatccttg cctcgttccg catcctctac gaccaggcgg
     1321 attttgacct gtgccgcatg gtgcggctgg tatccaccca gatgtaccgc tacagcgccc
     1381 cggagaattt cgcgaccctc ttcgtcgggg tcatcgatgc agcgacgcac gagatccggt
     1441 ttgtcaacgc cggccacaac cccccgatcg tcgtgcgggc cgacggacgc cacgaacacc
     1501 tgccggcttc cggaatcatg atcggagcca tgcccttcga tgcctggtcg gaggagcgcg
     1561 tcacgctgca gccgggggaa tcgctcgtcg tcttcaccga cggtgtcacc gaggccgagc
     1621 gggcggacgg atcgctcttc ggggaggacc ggctcgaggc ggtgttgacc ggggcgcggg
     1681 ggcaggcccc ggcccacctg tgtgaaacga taatgaagga gatccggcgg ttcgtcgaga
     1741 acgctccgca gtcggatgac atcacgatgc ttgttgtgca aaggaaggca tgatgctgca
     1801 gcccggacag gaatttgccc atttccggat catgcgggaa ctcggcgccg gcggcatggg
     1861 agccgtgtac ctggccgagg atctcaaact gcaccgcgag gtggcgctca agatcattct
     1921 ggcggagtac ttcaatgacg ccgagcggct cgggcgtttc cagcgggagg cgaagatggc
     1981 cgcccgcatc gcccacccca acgttatggg catctacgac atcgggagcg cgcccgaccc
     2041 cgacaccggc cgggacctcg agtacatcgt catggagcgc gtgaaaggca ttcccctggg
     2101 cgagtatttc cagagcgcca agcccgacat gggcggcgtg atccgggtcg cctcgcgcat
     2161 cgccgccgga ctggccgccg cccacaagct gaacatcgcc caccgcgaca tcaaggcctc
     2221 gaacatcctg gtcgacgacg aggggaaccc caagatcctc gactttggcc ttgccaagcc
     2281 ggtcgacccc gtccagttcg acgagggcga ccgcaccgac accgtgtcgc aggaactgac
     2341 gcgcgccggc aagattgtcg gcaccgtctc ctacatgtcg cccgagcagg cccgcggcga
     2401 cgccgtcgac atccgcaccg acatcttctc cttcggcgtc ctgctctacc gcatggccac
     2461 gggcgaattc ccctttgccg gccagtcgca ggtgacgacg ctggcgaaaa tcctcgaatc
     2521 gaaacacgag cccccccggc tgaaaaacca gcagattccg accgaactcg aacggatcat
     2581 cgacaagtgc ctgcagaaag atcctaatga ccggtacccg tcggccgcgg atttgacggt
     2641 cgacctgcgc aacctccgcc ggcagttcga ttccggcgtg accgagtcga tctcgggcga
     2701 gtaccgggtg gagaggacgg tccgggtcgg cgggaagcgt ctggctgcga tcgccgccgg
     2761 tcttgtcgtc gtcggaattg tcgcggtgat cgtctggcgc tccggcgggg agcggccggg
     2821 gggagccggc cccggcggaa tctcgccctc ggtgacgacc cccggcggca acgcggtcgc
     2881 ggtcttcgat ttcgagaaca aaacggggga cacctcgctc aactggctcc agacgggcct
     2941 gccggagatc ctcatgaccg acctggcgca gaccgggtcg atcacggtca tcagccggga
     3001 gcgcgtgctc gaccacttgc gcaccgggtc ggacctgccg gatgccgacg agatttcgga
     3061 cgacgtgcgg gccgaaatcg agcaggcccg ggacgagatt cgcgaggcgg tcaaaagcgg
     3121 gtcgctcgac ggcgtgccgg cggtcaagga tgcgctggaa ctggcgcggg tgagcgtggc
     3181 cggactgatg cgcccgcaca cctacgccga gatggcccag gcggccgggg cgctcggcgc
     3241 ctccacggtc ctctccgggt ccttcttcaa gctcggcgag cgtatccgca tcgacgcgcg
     3301 gctcgaagac atcggcagcg ggaagatcgt gctggcggag aaggtgatgg ggaacgatcc
     3361 cttcgccctc gtcgacagcc tcaccggcaa gatcgtcgcc tcgctcaatg tcaaggacca
     3421 gatggccgac cagtcggtgg ctaacgtcat caccgggtcg cccgaggcat acaggctcta
     3481 ccgggagggc atggacctct tcgggctggg gatgaacgac gaggcgatcg cgaagttcga
     3541 ggcggccgcg ggcgtcgact cggccttcgc gctggcctac atgcggatcg ccatggtcca
     3601 tgtcttcgag ggacggctgc agaaggggat gcagtatctg gcccgggccg atgcgttcaa
     3661 ggagagtctg ccggtgcgcg agcggtcgct gctggatatc tacaccgacc tgtggctcga
     3721 gcaggaactc gacgccgcct ttgtcaagct ccgcgcctat gtcgaaaact atccgtcgga
     3781 caaagaagcc cgcgtcctgt tcggactggc cacctgggtg tttaaccagg acaccgcggc
     3841 cgcccgcgcc cagtacgaca ccgtccttcg ggccgacccg gtcaacctgc gggcgctcgc
     3901 ctggacgggc gatctcctgc gccagtcggg gcagctcgat tcggccgttt actactaccg
     3961 caaggtcaag gcgtaccacc ccgattcgcc cgaggggtac acgaacctcg gcgcgctcta
     4021 tcgcgagcag ggggacatcc cgggcgcggc ccaggaatac cttctgatgt cggaggcgtt
     4081 tccgcgcctg gccgcgccgt ggatgaatct cagcacgctg gccatccgcc agcgcgactt
     4141 cgaaggcgcc gggcgcttcc ttgacgagta ccgcgggcgg accgggaaag atccctacgg
     4201 cctctacgag tacgagatgc agttgtcggg cctggagcag tggcgggggc agttccggtc
     4261 ggcccgcgac catatgcgcg cggcgctgga ctacgcccgg cagacgaaag acagcaacta
     4321 cgtgcgcagc gcctacaata ctctcttctc attctaccgg cgtctccata tggacgacag
     4381 cgccctgtgg tgcgcccggc agaccgagcc gtggtcgacg ggcatcgccc gcctgaatgt
     4441 cgccatgaat tacgccgagg ccgccccggc cgactgcggc cgcgcccgcg cgttcctgga
     4501 ggaaggcacc gcggctttca agcagcgcgt cccgtccggc ttgtggccgg tggccgaggc
     4561 cgtgggcgag gcattcgacg gctactgcgc gggcgacacg gccgccgtca tcgccgcgct
     4621 gctgaagctc agcagtgcgc agcagccggg gcagggggag ggcaaccgcc gcgaggccgg
     4681 cgtgctccag gtgctcacgg gacactaccg ggaagggcgc gacatccttg tccggtcgct
     4741 ctccggcaag gatgaggtcg cctccggagc gacctatccg tattttcttt acctcgtcgg
     4801 ggtggcggag caggaactgg gcgacaccaa gtcggccatc gcccgtttcg aggagatgct
     4861 gcgctactgg ggcaagccgg acatcgagac caaggagatc gccgacgcga ggcggcgcct
     4921 ggcggcgctg cgcagctagc cgcggcgggc cgtacgaacg cacagccggg gggatccctc
     4981 cccggctgtt ttgtctccgc gccggccgcc gaatcccgct tgtcaagggc cggccgatgt
     5041 cgttaattgc acgccactat gattgcccga agcgcccgct acctccgcca gttcgatctg
     5101 aatctctgga tcctgtcttt cgggtggttc aacagctcga tgggattttc catcagcctg
     5161 ccgttcatct cgatctactt ctattccgag ttcggcatgt cgatgaccga gatcggcgtc
     5221 tttttcggcg ctttggcttt tgtccggtcg ttcttccagg cggtcggggg cgaggcctcg
     5281 gaccggatcc agcgccggtg gctgctggtg tactcacagt atgcccgtgc ggtgtcgttc
     5341 ttggcgatgg cggcctcgat tcatttccac ctcgggttct gggcgacggc tgcggccctc
     5401 gccgtcaact cgatcctcgg cgccatattc cagcccgccg ccaacgccac ggtcgccgac
     5461 attctgccca aggcgcagca cctcgacggt tacgcgatca cgcggtcggc gggcaacctc
     5521 ggctgggcgg cggggccggc gatcggggga ttcctcgcgg gctggtcgta cgcggcgctt
     5581 tttgccgtct cggccctcat ggcgctcctg tccgggctgg tgttccacct gctcctgcgc
     5641 gtgcccgagg cgacccgggc ggtcgaccgc ttccgcctcc gcgacatcct gaccatccgg
     5701 gatgaccacc acctccttcg ccacgccgcc ctcctgttcc tgctctacct cgtggtggcc
     5761 cagctcaccg tgccgttctc cgtctacagc gtggagatgg ccggcatcag caaggtccag
     5821 ctgggcacgc tgtacacgat caacgggctg ctggtcgtgg cgctgcagat cccggtcacg
     5881 cggctgctca gccgcttcac gctgacggcg cagatcgcga tgggatcgtt cttctacgcg
     5941 gtcgggtatg gctcggtcgg gatactggtg ggatatcact atttcgccct ggccatggtg
     6001 gtggtgacga tcggcgaggt gatcatgtcg ccgccgacgc tcacgctgac ctcgcgcctg
     6061 gcgccggagg ggcgcatggg ccgctacatg ggcgtgcacg ggtttgtcgt cgcttcggga
     6121 tggtccttcg ggccgctcta cggcggcgtg attctcgacc atttccccca cacctggtcg
     6181 gtcgcctgga tgatgatcgc gtcgctggcg ctcgtctcgg gagccggcta cctgtggttc
     6241 cggcgctgtc tccccgaggc ggtgaacaag gcgcgctacg cgccgccggt caccgaatga
     6301 ccgcgaattt cccgatctgc gtgcggccct ccgactcgac cacccagtag tacaagccgg
     6361 tcgtcaggat ctgcgtgttg cgcgtgatga ggttccactc ctcgtgcgcg gaggaggcgt
     6421 cgccgggact ccgatcgtgc tcgatccgtt tgatcaggtc gccgtccagc gaaaaaatgc
     6481 tgatcgtgca tctcaagggg aggttggcga aatggagctt gcgcacgcgt tcgtcgggac
     6541 ggtcggagtc ctgccgcccc tcgaacccca ggccccggta gtcgccgtcc agacggtagg
     6601 gattcgggta cacgtaaaca tcgagccggt cttcagccac ggcggccgcc gcggtctgcg
     6661 ggtattcgag aacggcgttg atcagcgggc tcgtctcgag cggcggcagg ccgaccacgg
     6721 gtgagccgaa gtcgaaagcc gtgaccgcgg cgtagtaggg tacggtcagc agcaaatcgt
     6781 cgatgacgta ctcgtactcg tagtacttgg gcagcccctc gccgtgctca aaggtgaggt
     6841 cgtcgggccg ccactcggcg cggtcggcgg gaggccgggg gctgtccggg tataccttgt
     6901 gaatcccccg ggacgaggtc aggtcggacc ggttgttgtc ctggcgggtg aagtagtagt
     6961 ggctgccctc ccaggcgaac gaactgctcg gcgtgtagcg gagcgggtcg aagagcgaat
     7021 cgccgtattc aacgcgcaat tcgtcgagcg tcaggggcgt cccctggagt acccagtcag
     7081 accgggtggc gtcaaagaca tagcggttgt agttctcgtg gtcatacgac gagagaacgg
     7141 agaaactgcc cgggcggttg tcgcgcgcca ggtacacccg gtaaccctcg aaatcgacgg
     7201 cgttgagaaa gaagtcctcg gtggtttccg agtagtatcc gttccagcgg acaatcagcc
     7261 ggccgtcgga cggaatcacc cggatgcgcg gggcgacggg gggaccggcg ccgcggaaat
     7321 cgggcacctc gtcgcccacg taccactgga cgtcccaccc ggccccgctg ctgacggcgc
     7381 ccacggtatc gtcgcagttg tacaggcaca ccggggtgcc gtcggcaagg ctcgcctggc
     7441 agtgagcgcg tcccgcgtac ccgtcgctgt cggtgtcgac gccggggttg tcgtagaccc
     7501 aggatgccca cttggcgttg ccggcgagtt tgtcgaaacc caggtagttg tagaacgggg
     7561 tcgggttgta ggggtcgaag agcgtctcga acgcatccgc ctgggtatgg aagtcctcgc
     7621 cgccgagcca ggcgaacgag accggcagtt tctgccccgg attgatgtcg aaggggccga
     7681 aagagagcag gtagcggctg tcgtagccgc gggcgatttc ggccgcctgg tcggccggcg
     7741 ggggcatcca gacggcgttg ctcggcccga tcgtcgccgt gtagtacagg tcgtagtcga
     7801 actcctcgtg gcgcagcatg tagtacttgt tgcggtcgcc ctcggggctg ccgatgcgcc
     7861 ggccgaagtt gcggaacgga tcgctgtcgg tggggcgggt ccgcgggccg aaatcgcgcg
     7921 aggggtcgga gtagttgatg atccaccagt tgtaggagta tgcgagctcc gcggccgggg
     7981 tgcgcacgac ccgcacgccg acggcgctcc ggggagagcg atagtcccac tcgccgctgg
     8041 tctcgtccgg gtcgccgtcg ttgtcggcgt gccaggccac gttgatggtg tcgatgaacc
     8101 cgcaacgtcc ctcggccggg tgcgtccggt agaacccgac gacgtcgtcg ttccagccgt
     8161 cggggccgtt gcgcgtcacg tgccacacgt cgccgtcgac atagatgccg atgtagatgt
     8221 cgcggatcag tttctggccg atgttctcca cctggtagtc gaacaggatg aaatcatcgg
     8281 cataggcgta actccaggcc attgagcgct gcgtgatctt cacgccgagc ggcttgtgcg
     8341 gccggttgtc cgtgttgtcc gactccacga ttgcgggatc gtcgagcgtg tcggcgtagg
     8401 tgcagatgat atcctcttcg gagagcgcct ccgggtggta ccaggagctg ttgatgtcga
     8461 tcgagcggta cttgaatccc agcggcggct ggagatcctc ccagtcgtag tcgaccggcg
     8521 agaactcctc ggtgacgtag aaatcctcgt tgcccaccga cacgagcgtg tcgcggccca
     8581 cgaccgcgcc gatccagacc gcggccaccc agagatagac gaggtcggtg ttcttggggt
     8641 agacgcaggc ctggatcgcc tcgcccgtga tggggtcggc gatggttgtg ccgagcgtgc
     8701 cgaaggtgcc gttgttggcg accgcgagct gaatgttccc ccggctgtgg gcgatgtaca
     8761 tgatggtgcg cccctcggcc cgctggtgtc cgccgtagcg cgccgcgagg cgctccttcg
     8821 gcgtgcgctc ccgggcagcc gcgggcggcg gccaccacgc ggcggcgagg atcaggagga
     8881 aaaccagaca tcgtgcggcg gaacccagag gcggctgcat cggcttaccc ggtcacttga
     8941 taaccgccag cttgccgatc tgcgtctccc ggccctcagc ctccaccgtc cagtagtaca
     9001 gacccgacac cacgagctgg gtgttgcgcg tgatcaggtc ccactcctcg tagggggcat
     9061 tggggtccga cggcggcatg tcgtgctcga gcgtccggat caggtctccg tcgagagaga
     9121 atatccggat cgtgcagcgg ggcggcagat ttgcaaaacg cacgcaccgc acgcggtggt
     9181 ccggccggtc atcctgcccc cggccctcga atccgagcga gcgatactcg gcgtcgatgc
     9241 ggtacgggtt gggatagacg atgatcggga gctcctccga gcgcgccttc tcaggatcgg
     9301 acagcgggat cacgtactgg gctccctggg tgacggccga ttcgagcgac ggcaggccgg
     9361 accggggaga tccgtaatcg agcgcggtga cattgatcca gtacggcacc gaggggagga
     9421 gcccctcgat gtcgtactcg tattcgtagt acttgaaaag accatcgggg gccaattcgt
     9481 cggggtccgc ctggcgcggg tcgtcagtcg ccggcttggg ctggtcggga taggcgcggc
     9541 gaatggcgcc ggggattccc agcaccgagc ggttgaaatc ctgcggtgtg aaatagtaca
     9601 gctcgccggc gggtgtccgc agcggctcca ccatgctgta ggcgaggggg tcgaagttgg
     9661 cgtcgcggca cgagtcgccg aaccggcagg cgagttcctc gcgcgtgagc ggaaccggcg
     9721 aaatctccca ggtgcggcgg tcgggcagcc agtggaagct gttgtagttc tcccgatcgt
     9781 acccgcacac gacgctgaaa ctggtcttgc ggtcgtcccg tccgatgtag atgcggtaac
     9841 cctcgaaatc caccaggccc acgaacggat cttcggtcgt ctcggagtag gtgccgttga
     9901 agcggacgcg aaccttcccg aacgaaggta tgagctggaa cttgggagcc ggcggcgggg
     9961 ccgcgccccg gaagtccggc actccgtccc ccgtgtacca gtggatgtcc cattccgcgc
    10021 cggccgcgtc cgcgccggtc gtgtcctcgc agttgtaaag gcagatggcc gtgccgtccg
    10081 acagccgcgt ctgacagtgg gcccggccgg cgaagccgtt gctgtcggtg tcgacgcccg
    10141 ggttgtcgta gatccacccc gcccagcggg cgttttcggc gaaatcctcg aagtgcaggc
    10201 tgtcgtagaa agcgtcaaag ttgtagggca ggttgctcag gttctggcgg taggtgtgga
    10261 aattcttgcc gcctacgtaa gcgaacgaga gcgggagggt ctgccccggg cggatgttga
    10321 agggcccgaa cgagagcaga tagcgcgtgt cggcgccgtc ggcccactcc gccaccagct
    10381 cctgtttcgg atagacccac aggggatcgg tggggccgat cgacgccacc cgcgcctggt
    10441 cgtagtcgag ctcctggttg ctcatgatgt agtacttgtt gcggtccccc tccggcgtcc
    10501 cggtgccgcc cgtgccgaaa tcccggaacg gctctttcca ctttccggta taggggcgct
    10561 cgcgcggccc gaagtcgagt tcgccgctgg ccgagaacca ccagttgaag gaaatctcca
    10621 gcgtgtcggc gggcgtccgc acgatgcgca tcccggtcac gtggggaacc gggcgcggga
    10681 aatccaaatc cccgtcgttg tccgccagcc aggcgatatt gatggtgtcc gcgtacgagc
    10741 aggggccgat gacctgcgag tacttctcca aatacccggt gaggtcgtcg ctgtacccgt
    10801 tggtggtggt cgggtggtac acgtcggcat cgacgtagat ccccaggtac acgtcgttga
    10861 gcatctgcga attcatgctt gtgatctgat agtcgaaaag gatgaaatcc tcggcgtagg
    10921 agtaggacca cccgtaggag ttctcggtca ccgcgatctt gatggggatg tgcggctggc
    10981 cgtacaggtc gggcgggatg ccggccgtaa acgtgtcggt gtaagtggcg acgaagtcct
    11041 cttccgaaac cgcgccctcg tattcgctgt cttcgggatc cacaatggag cggcggagca
    11101 ggggtcggcg gaaggggaac atttcgcgtg tcgacgacca cccgtcggcg cccagcgaga
    11161 cgaccgtgtc ccgccccagc accgccccta cccagaaggc ggccgagaag agatactggg
    11221 tgttcgagcc tttcgggtac tcgcaggaat acacctgtcc gccgctgaag cagtcgccgg
    11281 tggagccgag cgtgaagccg gtgccgaaag taccgttgtt gttgatggcc agcacgattt
    11341 cattgacccg gtgcgacgcc acgcagagcg gcacctcggg ctgttgcgcc gcctgcgacc
    11401 gaccgcgccg ctcctccgat ccgatgaact ccgcgcgtcc ccaggcgtcg gccgcaagtc
    11461 cggcaccgag cagacacgca agtgcagata ccgtcaccgc tttccgcata tttccgccac
    11521 tgcttccccg aaagggttcg gcatgtcttt tgaatgccac cggttgaaac cggcgccgcc
    11581 gcacggagcg gcccgccgtc accatcatgg gcaaaactac ggcatggagc gtgcgttggc
    11641 aagacccttt tcgtcctatt actaaaggaa agcggcgccc gaatgagcgc cgcttcagga
    11701 atctgccttc tgtccgggtc agcaagcgca cgggtcgggc ccgctgcgga acaggtagtt
    11761 gatcaggtag gtcagatccg acaccttgac cgatccgtcg cagttgacgt ccgccgtctc
    11821 cagatgcgcc ggcggcggac cggaccggaa gaggtacccc accaggtagg tgatgtcggc
    11881 cacgctgatt cccgggctcc cgtccagatc ccctcgcggc gggcataggt cgagcgttcc
    11941 cgcctgcaga accggcgtat acgtggccag cggcccgctg aatgccggca gataagtacc
    12001 gtagccggtc agcttcacgt cggctgactg cgggaattcg gcttccggtg agatgacgaa
    12061 gaccaggttc acaatcggtc cggccccggg ttcgagcggc ggggcggtcc cggtcaccga
    12121 gctctggagc cgcaccgcca tgcgccggcc ccccggaaag ctgtaccaca ggtcctgaac
    12181 ctggaaatat gaggtccgac agccgaccgt gttgaaagtc gcactcgaaa tcaggatgtc
    12241 gccggggtac tcgacgggaa tgtcaatgcg ggtcaagggg acgttgttgc gggcgtagat
    12301 cggcacgatg accgtgtccc cgcgcccgcc ttgggcggcc ggggcgacca tcgtgtcagc
    12361 cagaaccgcc acgtagtcga cccgctgggc gacccgcggc tgcccgtccg ccatgatttc
    12421 caggcgaacc gtgtactgcc ccccggcctc gtaggtgtgg accgggtcct gaacgaacgc
    12481 cgaatcaccg tcgccgaaat cccaagtcca actggtcact tcaagtccgg aactgccggt
    12541 gaacgccacg tcgaacggcg cccaaccgat cgtcgtatcg gcgctaaacg agaccccgat
    12601 cacctggtcg gtcgcgccct ggaggtcggg gagcaggccg acatgtttgt agtgggccgc
    12661 ctggggtgtg ccgtacgttt gcatcaggct gcggatttcg ttgtggtata atgtggtgcc
    12721 gtgggtcgcc ttgttgatcc cctggagcac ggccgccgac ccggtgatga tcggcgaggc
    12781 cgacgatgtc ccggagaagg aaccggtgta cgaatcattg atgccggtgc cgtagagcga
    12841 cccgtagccg agggtgtaca catcccaggt gccgaacgca tgcacgtcga cccgctggcc
    12901 gtagttcgtg aacgacgcgg gcacgtggcc ggcgctggtg gcgccgacca tgatcgcacc
    12961 cgagaagcgg taggcggggt cgaacgtctg gccgtagatg ccgacgtcgt ccaggttttc
    13021 gttgccgttg ccgccggctt cgacgacgat ccggccgagg gccgaggcct ggaggatggc
    13081 atcgaagacg tcctgccagt actccatggg gacatacccc gcctggtcat cgcggggttc
    13141 gaaatcgtag tgcggaccgg gcgcgtgcaa ttcgatgagg aagacgtcgc cggcttcgga
    13201 atttgccacc gccgtcgtaa tcgccgccgc cgtcgacatc gagccgatcg agacggtccc
    13261 gagatcgacg ttgaacgcga tgccggtcat gccgaaggaa ttggagtcgg cggcaatctc
    13321 gccgagcacc gccgtgccgt gattccacca gccggcgtcg ttgatccggg tgccggcgat
    13381 gtggaaattg tccgtgccgc cgtggagatc ctcgtgcgtc tcgatccagt tcccctcgat
    13441 gtcaatcact ctgaccgttt cccccttgcc gccggggtag ttccaggcgt agtacgcgtt
    13501 cacgccgatc ggggcggctt tcaggtagta ctggttgttc tcccagttcg gggtcgcctg
    13561 cacttcggcg atcgacagca tggccgggga gagggatctc ccggaggccg gttcggggtc
    13621 gggctgaaag aaggcgattt ccacgaggtc aagggcgttg aggtcgttga ccgccgccag
    13681 tttctcggcc gccgtggccg aggcggggaa ccgcagttcg tagtaccggg tgaggtcggg
    13741 catcgcccag ccgacgcggg ccgaggcctc ctgccgcatc cggtcgagca cggccgggtc
    13801 cagcggaata tgcggcgcca gctcgcgggc cgctttcgcc gggccggcca cgattgcgtt
    13861 cagccggtcc cactgcgccc cggactgaac gaagcgtccg cccgcgaccg cgggccgatc
    13921 gggcgcctcc gcaagtttga acaccacccg gccggcgcca tcgccggcgc gaagagattt
    13981 gagcgcgctt ttgaccggaa ccgggcgctt gagcgaggca tcggcggcgc cgctggccgc
    14041 accgctccac cacaaaacga gactgaggca gcacgctgcc acaagggcaa tacgcttgtt
    14101 cacgatatgg ttacctcggc agtaatgaag tttctctgtg agcagatcgt cctggcggtc
    14161 acaccaaaga taatagccat ctcgaattct gcaacaagta ttccgttgcc cggttccctc
    14221 gcgacggccc gtctcagtcg gccggcggcg ggcccccgcg aaacagaaac gccaccatgc
    14281 aggtgaggtc gccgattgtc ggctgcgccg gatcattgcc cgacccgtcg cagtcgcacc
    14341 ggaccgaggg gcaactgccg ccccggaaga gcaggttggt caaacaggtg aggtcgccga
    14401 tgccgtaggc gccgtcgccg ttgaaatcgc acatcagggg ttccggcaag accagcttga
    14461 tcgacccggg ccccatcacg accagcgtcg tatccatctc gtggacgtaa atatcgtaga
    14521 catagatcga atcccagtcg gccgtgtccg ggtcgatctg aacccacccg aggcagatat
    14581 ctccgtccca ggcggtgcag cggtagaaga gcgtgtcggg gacggtgtcg accaccacca
    14641 tgccaatgct gttgccgcgg ggatcggaga aggtgtaggg accggtgacc gtgatgttgc
    14701 aggtcaggcc gccggtggtg tccggttggg cagccgtgct gaagggcagg cgggccagaa
    14761 cggccccgtt ctgggcgggg atcccgggcg tctccggtcc gctgaacggc aggttggcta
    14821 gcgcgatcag ccggtaaatg ctgtggtctg cgctgtcccg ggccaggacc agctcaaatc
    14881 cggacagaag ggcgccggcg gtgtcgaaat ccggtccact cagatcgaac cagacaaggt
    14941 ccgggcggtc taccgatagg cgaaattcaa ggcccgcaat ggtatcgaca taattgctca
    15001 agtataaatc caaccaggcc gccgactcgc ccggcgaggc ggtggtgtcc tggacatcaa
    15061 atacgattcg cggagaatcg gccgccgttg atagcggggc catcaggact cccagcacta
    15121 ggaaggccag gctaattcga tgcgcgtacg ccatagggtc aatcctcgtt caacttgcct
    15181 gtttcgcaac gaattagcgc tggttctcca gtcggatctc ctcggccacg ggtgcatggc
    15241 ggaccggccg ttggtagtag tatcagcatc cgcaagatag ggcaaatcag gaaattgtca
    15301 acgccaatcg ggcaacagaa agccggccgg gagggcccgg ccggcgcgat ctgaccaatg
    15361 ctcgaaagcc aaccgctcac tgcggcggcg cgaagacctc ggccgctatc gcggtgttga
    15421 cgtcgaacac gctgatattc tcttccatga tcttctggcc gttcagttcg gtcaccgtaa
    15481 ccgtcggaag agtgatcttg cctttcggct gcaggttgga gtacttctcg gtgaccatac
    15541 cctcgcccat catggtctga tcgaagtaca tcttggcggc cagtgtatga ctgccggcgt
    15601 caaagccgaa gcgggcgagc tgctcgccgg aggcgtcgag gatggcgacc cattcgaccg
    15661 gggttccgcc gagctctcct cggccgttgt agaccgcctt gtagtacgga ttgccatagg
    15721 cctggaagac gcggatagtc tcgcggaggc gctctttctc gccttcggcc acttcatccg
    15781 ccgacagggg caccatctgg ccggtttgat cggtcctcca gccctgctgg ccgacccgaa
    15841 ggctgatgat cttctgaccc atgatgctga tttcggattt ctccttgtcg gggatgactt
    15901 cccagctgtt gactgtcgcg gggagctcct ggccgcccat cacgagcttg atggagcggg
    15961 tgctgacgac actggtgacg ccggcgaagg cctccgtccc gccgtgggcc gcaatggcct
    16021 tgcccaggat ctccatcccc ttggcgatgt tcgcatcgtt gacgaccagt tcctcctggt
    16081 cggcctgctg cggcgccgga atcgatatgt cgaccgcctc gatcggaccc cagccgagcg
    16141 aatcgagcgg caggtcgaac ttctccttgt cgccgacgac caggatgacc atggagtccg
    16201 ggtggagact gcgtttcgcg gccgccacga catcgtcctt ggtggtctgc tccacgcggg
    16261 ttttctcgcg ctggaggaag tcctcgggca ggccgtagaa gtcgtagctc atgatccggc
    16321 cgatcacctc gcggcgggaa tcgaagttga acacgaacga gttcaggtat ccgtccttgc
    16381 ccttcttcat ttccatgtcg gtcggcggca ccgtctgcat cgagcggatc tgctcgatca
    16441 tggcccggca ggctttgacg gtgttccccg gatcggtcga ggccagcgcg aagaagtacc
    16501 cgggataggc ggcgttcgac acgtagcgcc cgccggccga gtaggccaga cccaggcggg
    16561 tccggacttc atcggtgagc cggcttccga atcccagccc gaggatcgaa ttcatcacga
    16621 tcttgtcggc atagtcgggg tctttcagca tgccgccgag atgtcccatg cggatgtacg
    16681 actgcttggc gcctttcttt tcggcgtagt acacctgttt gcgccactgg tagtcgacca
    16741 ccgggggcgg cggcactgcc gcggtccctt tcttccaatc gccgaacagc ttcttgaccc
    16801 gggcgaggac cttgttcttg tcgaagtcgc cgtagatcgc catctggatg ttctcgggac
    16861 gcacatagcg gtcgtggaaa gcgaccaggt cgtcgcgcgt gaccttgtcg agcgtggcgt
    16921 actccatagt gcgggcgtag ggcgactcgg ccccgtagat cagcttgcgg aactcgcgga
    16981 gcgcgatctc ggaaacctcg tcgttgcggc gcgcgatgtt ggttcgcgcc tgcaccatgg
    17041 ccaggtcgat cttgtcctgg tcgaaccggg ggtagcggag aacttcggcc agcacaagga
    17101 ggccggtgtc ggcgtagtcg ctcagcacgt tgacgaacgc gccgccctcg acgtccccga
    17161 aattggtctc gaccatgccg ccgattccct cgagcagctc gtcaatctga tcgccggtcc
    17221 aggtgcgcgt gccgcccgtc cgcatgacgg tgccggtgat ttcggccagt ccggcctgat
    17281 cgcctggctc gaggtaggag ccggcggcga ggcgtacgtt gacgttgaac agcggcagcg
    17341 acttgtccgt cagcaggtag agccgcaggc cgttgtcgag ctggatcttc tcgactttcg
    17401 gaatctccag cggattgagc ggcgggaagg tgatgctctc gacgtcctgg gcggccgccg
    17461 gcaacgcgag cgccgccagc caccacaggc acaggagggt cgtccatttc ttcatgacac
    17521 actgctcccc taactctgaa ccgtgttcag gcagacgacg gtccggttgt tcttggtgaa
    17581 gtacttcttg gccacgcgct ggatgtcctc gggggtaacg ccgttgatcc ggccgagctc
    17641 cttgaacagg gcgcgccagt cgccccagtc attctgggcg gaggccagct gcgaggccat
    17701 gccgaggttc gaggtcaggc cgttgatgaa gtcggctttg gcgcgggcct tgatctcggc
    17761 cacgtcctcc gcggggagca gctcgttctg cagccgctcg acctccacgt acacctcgct
    17821 ctcgcactcc tcgttggagt ggtcggcggc cgggatgacg ccgaccaggg ccagcgtcgg
    17881 gtatttcgag gcgggatagc cggcaaaggc aaacacctcg gcggcgatct tcttctcctt
    17941 cacaaggttc gtgtacagga gcgacgtgcg gccctggccg aggtagtcca tgagcgcctg
    18001 caccgccggc caatcggggt gggtgtactc cggcacgtgc catgccgcgg cgaagaacgg
    18061 ctgagccgga tcctcaagga tcacccgccg ctcgccctgc tgctcgggtt ccaccgtggc
    18121 gaccggctcc ggattcggct tggccggcat gccgccccag tactgctcgg ccagccggaa
    18181 gacctcgtcc gggtacacgt cgccgcaaat ggcgatgatc atgttggacg ggacgtagta
    18241 cttctcaaag aaggccttgg ccgcgtcacg cgtgtagttc ttgatgtccg acatgtgtcc
    18301 cacgatcgag atgccgtagg ggtgggccgt gaaagcggtc gatttcagga cgtcgatcgc
    18361 ccggccgatc gggttgttct cgagcgtctg gcggcgttcc tcggcgatca cctcgcgttc
    18421 gcggtacatc tcgcggagca cgggctggtt gaaccgggcc gactccatcg ccatccacag
    18481 ctcgagcttg ttcgagggca ggctcatgat gtagctggtc tggtccatgc cggtcccggc
    18541 attgaggttg accgccccgt ggcgctcgag gatgttgtcg aattcgttgg gcacgacgac
    18601 ccggttggcc gcctcgatgg cctcgtcaaa ggccttctgg agagccgcca ggcgggtcga
    18661 atccgcctgc gcgccctttt tccgctcggc gcggagctcg tagaaaatcg aatcctccac
    18721 cttcatcaac tgcagttccg tgtcgatgtc ctgggtgccg acctggcgcg tgcctttgaa
    18781 ggccatgtgc tcgaacatgt gcgccagtcc ggtgtactcc ttcgggtcgt ccacgccgcc
    18841 gacatcgacg tacgtgacga aggacgcgac cggcgcctcg tgacgctcca tgacgatgat
    18901 tttcaggccg ttggcgaggg tgtgttccga caccgtcttc tcgatcttct gaaaatcgaa
    18961 ggcgaagacc aacggcgcgg agacgagggc gaggcaggcg atcgccgtgc ccgctcgcag
    19021 gatcatgcgt ctcatcagta cctccatata gttatgtcgc attccggcaa aagtcagact
    19081 aatgtaggga acgggggaga agctgtcaag cggcaggctg gcggcggcgg ggggggcgtc
    19141 ccggcgagcc gcggacgggg cgggcgccgg acaaaagaaa ggcggcaggc cgaaagcctg
    19201 ccgccgccat ctgccgccgc actcggcacc ctaccaccaa ctgatgaggc ggggtttgaa
    19261 gacgtggggg agtgcgtcgt tgcgggggat gatccgcgcc ttgatcccga accggcccga
    19321 ctccgtgcac tcgacctcag cggcgaacgt gtacgtgccg ccgccgtcga ccggcttgac
    19381 cagcttggcc ggcaccgagc ggtagttcac gagctgctcc tgagagttca gccgcccggc
    19441 gaccagttcg acagacacgt cgtcgggcgt gatcgccccc aggaaaacgt cggcttcgat
    19501 cgggagtttc tggcccacgt agacggtggc gccgacgtcg ggaatgcgga cgtcgttgat
    19561 cgccagcgtt ccccagccgg cccgcatccg gtcgagccag gcggtcaggc tccgggtctt
    19621 cgcgaagttg ttgtcgcgga gctggcgggc agagtggatg gcgcggacgt agaattcgtc
    19681 ggtgtaattg cgcagcatcc cctgggccgc gaactcctcg ccggccatct gcacggccgc
    19741 cttcatcttc tggatccact tctccggcag gccgacgccg ttccggttgt ggaagagcgg
    19801 caccacctcg cgctcgagca cgtcgtagag catttcggct tcgaggcggt cctgaatctc
    19861 gacgttttcg tactcctcgc cgttgccgat tttgaagccg cgctcgtcgc tgtacccctc
    19921 ggcccaccag ccgtcgagca ccgacacgtg gagcgccccg ttgagggcgg ccttcatgcc
    19981 cgaagtgccc gaggcttcct gcggccggcg cgggttgttc agccagacgt ccgctccctg
    20041 caccatgtag cgggcgacgt tgatgtcgta gtcctcgagg aaaatgatgc ggttgcggaa
    20101 gcgctcctcg ctgatgacgc cgacgatgta cttgatgatt tccttgccgg ggttgtccag
    20161 cgggtgcgcc ttcccggaaa agatcagctg caccgggcgc ttttcatcgt tgaccaggcg
    20221 ttccaggcgg gcgaggtcgg cgaagatgag gctgccgcgc ttgtaggtcg aaaaccgccg
    20281 ggcaaagcag atggtgaggg cctggggatc gagcaactgc tcggcgcgct ggatatacag
    20341 ctgcgaggcg ccccggcgct gcagctgctg tttgaggcgc ttgcgggcga aaaagatgag
    20401 tcgctcgcgc cgccggttgt gggtgcgcca gagctccatg tcgggaatct tctgcacccg
    20461 cttccacacc tccggcgccc ccggccgctc gcggtagcgg gggccgaggt acgattcgta
    20521 gagctcgtcc atgtcgtggg aaatccacga ctgcgtgtgc acgccgttgg tgatcgcctt
    20581 gatcggaact tcctcaatcg gcaggccggg ccaaatgttg tgccacatct cgcgcgacac
    20641 ccggccgtgc agtttcgaca cgccgttggc gaaggcggac gtcttgagcg cggccaccgt
    20701 catgccgaac ggttccgatt catcctccgg catcacccgg ccgagggtga gaaaatcctt
    20761 ccacggcacg ccgagcgaag ccaggaagtc gccgaggtag cggtgcacga ggacggggtc
    20821 gaactgctcg ttgccggccg gcaccggggt atgggtcgtg aacaccgtcg tcgcccacac
    20881 atactggagc gcctcgttgt agcttagccc ctgctcctgc atgagaatcc gcagccgttc
    20941 gagggcgaga aaccaggagt gcccctcgtt catgtggaag atggcgggct cgatccccag
    21001 cgcccgcagg gcccggaccc cgccgattcc cagaaggatt tcctggcgga tgcgcatgtc
    21061 cttgtcgccc ccgtagagga ccgatgtgat ctcccggttc ttggtcgaat tctccgggat
    21121 attggtgtcg agcgcgtaca tcgggatgag gccgacggtt gtcttccaga cctggacgtg
    21181 caccggggcg ccggccatct gcacggtgat ccggagcggc tggccgttgg cgtccttgac
    21241 cagctccacc ggcatgtggt accagtcgtt ctcctcgtag cgctcctgct gccagccgtc
    21301 ggacgacagg aactggcgga agtagccgta gcggtagagg agcgccaccg cggtcatggg
    21361 gaggccgatg tcggaggccg
//
LOCUS       DAOPOE010000052        21228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3079_length_21228_cov_5.169981, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000052 DAOPOE010000000
VERSION     DAOPOE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_21228_cov_5.169981"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKM94_08215"
     CDS             <1..567
                     /locus_tag="PKM94_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD66662.1"
                     /translation="ELGALLAFSAIKNNDKVGLIIFTDQIEKFVPPRKGRGHVLRLIR
                     EILFFEPKRTGTDVAGALEYLNRVVRRKSVVFLISDFLSEDFHRPLQVANRRHDLIAV
                     KISDPRESRFDDVGLIELEDAETGEVYVIDTGSKEFRREYAARADEDLGRLEREFRLI
                     NLDFINIRTDQPYIKPLIAFFRSRERRL"
     gene            569..1477
                     /locus_tag="PKM94_08220"
     CDS             569..1477
                     /locus_tag="PKM94_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66663.1"
                     /translation="MAGRRATALMLGLAWAAAIAGCGGSTAHEAAIERHKKLAAELRD
                     NRLYDAAVEEYRQILAYDDLDDGTRAGVSYLIGRLYFENIGDYEQAAAYYVRARALDP
                     DGDFVLEASKNLVTALERSGNLVNAKRQLDVLTDIGAAPAGDSDVAVARMGGVPVWQS
                     QVDDAMEMLPPEAQKEYTRDREARVRFMRQYVAAELLYHAAIREGFDKDAEIARRTRE
                     LNRQLLVEKYVREKVAPQIQIDTLDLRNFYLANKDSRYGGAPFDSVQARVLLDYQSQK
                     AESAFQDYLALLAQKERVEFLDRNVK"
     gene            1485..2558
                     /locus_tag="PKM94_08225"
     CDS             1485..2558
                     /locus_tag="PKM94_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOD66664.1"
                     /translation="MRTRRPYRLAAMAAAALALGPAAPGAFGQAAGAAPGDTVGSLPG
                     VEVRTSVDRAKIFVGDQVTYTLTIIHDSTIDLVPPPLGANLGAFQVLDYETDRVTRLP
                     DGRVQNESRFVISTFTTGEYAVPPLPVMAIMPDSSRKVLLAEAVPITVESILAAGDTL
                     MDIKPLAGVLGPEAFAKDRTWWYVGGGAAAAALLAALLWALLRRKRKPAAAAVDLRPP
                     WEIASERLALLLHKNLIADGRFKEYYYELSDTLRWYLGRVYDRNVLDWTTEEFLTAFR
                     EIELPHSLYDGVEAFMSHADLVKFAKYEPSIEQTKYDFEFVHAAVDRVRQDVFRRQQG
                     QAAAGGGRPAQPVGAAGGGTHES"
     gene            2548..3591
                     /locus_tag="PKM94_08230"
     CDS             2548..3591
                     /locus_tag="PKM94_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD66665.1"
                     /translation="MSLEFAGFSVNLLDLLETTVSPGAIFATGGAIVLAMLAYYFTRR
                     RAAAAAIKFSDLRIVKRAGRTGRQRFRFVLPVLRVLAVALLVVAFARPRSGTEYTEVT
                     SEGIDIMMALDVSSSMQAEDFKPHNRLYVAKEEMKKFIGRRINDRIGLVVFARYSYTQ
                     CPLTTDYGVLLSFLDQIDFGLVEDGTAIGMAIANAVNRLRESEAKSKIIVLLTDGDNN
                     AGEIDPLTAANLAATFGIKIYTIGAGKPGNAMYPVDDPLFGKRYVYRPTVIDEETLRE
                     IAARTNGKYYRARSGEELDQIYAEIDRLEKTKVETAQHIQYVELFQHFAFGAFGLLVL
                     EMLLAHTYFRKLP"
     gene            3606..4628
                     /locus_tag="PKM94_08235"
     CDS             3606..4628
                     /locus_tag="PKM94_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD66666.1"
                     /translation="MRFADPGNFVLLALVALLALFAGFALARKKRLLARFADIPLVMR
                     NAPYISFARQAGKAALLLLGMAFLTLALTRLQFGTHLEMLKREGIDLVVALDVSNSML
                     ARDMAPNRLEKAKQELQGIIGRLKGDRIGLVAFAGEAYIQCPLTLDYTAAEFLLGAMD
                     QNSVSVQGTSIGSAIDMAARSFEQKERQHKVLLLLTDGEEHDRGAEAAAEAARREGIK
                     IYTVGIGNPAGEPIPLFDRRGAQVGFKKDDNGEVIITRLNEELLQKIALATGGKYYHA
                     TAGEMELDRIFGEISEMEKKELEGTLVTRYDDRFQWPLLAALALVVGEFFVSERRRVP
                     RKEHHA"
     gene            4621..5382
                     /locus_tag="PKM94_08240"
     CDS             4621..5382
                     /locus_tag="PKM94_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66667.1"
                     /translation="MRRLVAIVLLAALAGPARADFLDEIRDGSRAYAAGDYKKAMEHF
                     HNAEADRPEDPRVQYNIGGTAYEMGDYEMAEEYLSKALAAADAALEAKAHYNLGNTYY
                     RMQDYPKAIQSYENALKLDPNDTNAKINLELARRMLKEHTKPEQQPNQQQNQPQPQQD
                     QQQQQQQQQPQDQAQPDQQQEQQQQQTGDQQTEEQPQEGQKEQQQNQQEMKEQQVSKE
                     DAERILNALRDDEREIQERLKRRVQARGNYHGKDW"
     gene            5396..7213
                     /locus_tag="PKM94_08245"
     CDS             5396..7213
                     /locus_tag="PKM94_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOD66668.1"
                     /translation="MRRGCGLGPAAALAILLWAAGAAAQGTGEVRVEVSLKPDSIGLD
                     EQALLAVTVSGAAQNLPAPDMPSLPRFEVYSQGSSSNITIVNGKVSASMTYRYLLVPT
                     KAGVYPIERIAVVYNNRRYEGNRVTLTVLDKGTAMTPEIRRQTEESGEGAKDYFLEAV
                     VDKKQPYVNEQVTLTLKFHIAVQYYGSPELIEPTTTGFWTEVLGNEAPYRQQIGGRAY
                     RVIERKYALFPTQTGELTIGQAAIRVTVPERRQSTRDPFGMFNDFFSSGREVTVRSKP
                     VTVNVRPLPTAGRPDDFTGSIGKYELTARADKNEVEVNQPVTVTFQVSGTGNIKAAAK
                     PPIPQTDDFRVYEASSSENVGKQGDRLAGTKIYEEVFIPRRPGMLEIPAITYSYFDPE
                     RGAYATTSTKPIAIQALKPEGYVASGDLPYQGGGVVVGSEARDIRYIKEDIGRPARSG
                     RIILLTPLYLLANGVPVLVLAALIAVRKRREKLAANVGLARSLRAGKMARKRLAKARG
                     LATAAQGQAFYAELHQTLTAYVADKLNVSPHGLTTERLRELLGQQGADEGLVAAVADI
                     LQRCDFARFAPASISQEEITRALADVEETMIRLEGIHFA"
     gene            7206..7958
                     /locus_tag="PKM94_08250"
     CDS             7206..7958
                     /locus_tag="PKM94_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66669.1"
                     /translation="MRKLVLMIGLAAMLAAPAGAAEVAEAFRQANARYEARDFAGAAG
                     AYEDILKSGTASAQLYYNLGNAYFKQGDVGRAVLNYLRAQRLDPRDEDIRHNLEFARQ
                     FARVKMEGVELNPISSFFASLVGPVRLNLLAWTSSALFALFCAMLAIRYGLGFRGGWV
                     RLGVVAALVLATAAAGLTTYKYRTEYLTRRVVVVADESPVYNGPTANADVEFQGAPGL
                     VAEVLGESGAYLNVLFENKRRGWVRRDLVAEV"
     gene            8051..9091
                     /locus_tag="PKM94_08255"
     CDS             8051..9091
                     /locus_tag="PKM94_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD66670.1"
                     /translation="MIWKKKKFWGTLVAVALLAFCVKDISLTDLRSLSVRIDYIYLIP
                     ATVCTFLYSVFKALRWRLMVKTQKTIPAWNAITLYSAGQVINVVMPALTGQLGRILLF
                     ARAEGLRKTFVFSTIVLEVLLDAITLVAALLLTSLAFAIPDRYRTAGYIIGLVTLVLI
                     GLLYLLLHYEDRAENFGGAWCRARWPKFHAGMQKSIRSFTSGMELLRSSQNVCLSLGL
                     SFLYWGAHIFVVYFLFLAFGFGLPFSAAAMVMVINTLALMVPITPGNAGTFEVAVSTA
                     LLAFRVARPDAVLFALALHLLDLLPLWLLGMWFLRTEKVSLKDIREQHEDRLILDEMD
                     ERGSLIEREDPR"
     gene            9088..10056
                     /gene="corA"
                     /locus_tag="PKM94_08260"
     CDS             9088..10056
                     /gene="corA"
                     /locus_tag="PKM94_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOD66671.1"
                     /translation="MIRSFLYLPGEGVDTREGIADFREMTEKEGAILWVDLCKPSDEE
                     SYVLTHDFNFHPLAIEDVISEKPRTKLDDYDRYLFLVFQIVDYVGREEGLKIAELDLF
                     LTQNALVTVHYDDHRIYDYLYHRAERDERLMSRGVDFLFHAVIDAVVDNYNATLDILE
                     YEIDKIEDDVLEDLDQETLRSIFTLRRDIIQLRRIVIPQKEVVLHLSREQFPQISTRA
                     AVYLSDIHDHLVRIDDLANFHREILNSSLEIYYSSISTRTNEAIKVLTILSVLFIPPT
                     FLVGVWGMNFRNMPELEWRYGYGAALLFIFLIIGGLLVYFRKKKWI"
     gene            10132..10770
                     /locus_tag="PKM94_08265"
     CDS             10132..10770
                     /locus_tag="PKM94_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD66672.1"
                     /translation="MANRQVKKRIRQSSKRPAGERRDQLLRAAHELFLRQGYRATSTD
                     EIARRAGLTKGAFYFHFASKKDILMTFVQDFVEGHTRAMERLAGKRLTPAGLLAELGR
                     IDARMPLREARKNLSLLAEIMYLPRFRALIRRAYERSVAVMAECLDPGYGRTAAQRRR
                     LVELMHAIYDGVCFAGMVHPQLGNFERQLETFRRLTAKKRIRSNRKHGPAKS"
     gene            10751..12148
                     /locus_tag="PKM94_08270"
     CDS             10751..12148
                     /locus_tag="PKM94_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD66673.1"
                     /translation="MVPRKVEKPGRAAGPVRRWGGVVALAAALAAAGAAAAADGARPD
                     TMVLTAAEVRRLALEYNRDYLQALEEVRAADAEVVKARAGALPDISGQAYYDRNFLIA
                     SNFVQMGDETVEFRFGFKNSFGAGISLRQPIYQGGKVFTAVSIAQQYRKYARAGAAEA
                     AAEVAYTAEVLFYQVGLRESQVNVLRQALEAAELNADMVQKQFDQGMTSEFEVLRARV
                     ERNNLLPQLIASESEVELSRKRLKSFLGLDLSRPIRLEEPGADADTSLAGLGPEPAYV
                     DTALARRPAMQQADLLVDIAKKAVKIAQADYWPNFEAVSSWGWSAVSDDFTLRENNSR
                     SLTAGLMVSMPIFDGLRRSGQVTQAKVDHNQAALAASKIRDDIRLEVEQSYDALVQAK
                     KALDIQGTNIAQAEEGLKIANVRYESGVGTLLEVLSAQVALTDARSARVQALFAFRVA
                     QAKLKRASMIQPSME"
     gene            12153..13193
                     /locus_tag="PKM94_08275"
     CDS             12153..13193
                     /locus_tag="PKM94_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66674.1"
                     /translation="MQMRRMILAGGAAAAAILAGCGGAQQDTAQRPEVPVRVQVLAPF
                     DGAVTKTYTGALEGQNQAAIYTKIAEAVESVLVREGGRVQAGQVLVKLDRTGSMSNYQ
                     QALSVYRNAEKNYTKMKTLYGQGAVSEMQYDAARTEYEVAQASFEAARRLVEIQSPIS
                     GIVTSVNVRRGDYLAQGQMVATVAATERLRVRFQVKATDVGLVQVGDTVRVTAETAAE
                     SLPGVVAAVAQSADPASRAFEVEALVANAGESSFRPGMFVRVELVMERLQGVLGVPPS
                     AVVALSSGAVVYVVENGLARRRPVTLGTEAGGRVIVSEGLRPGDSVIVLGQSYLDDSA
                     AIRVTDVEKGAR"
     gene            13190..16342
                     /locus_tag="PKM94_08280"
     CDS             13190..16342
                     /locus_tag="PKM94_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD66675.1"
                     /translation="MKLSEVAVGRPVFTTMMMAALLVLGLASYTELPIDLMPDIEFPY
                     SVIQTVYPGASAETVESEVTSPLEDEVNLISGIRHIESRSLEGYSLVIIEFELERDAA
                     EATQEVREKVAGIRSDLPDDIEEPMVLNFDPTAFPIMSLAVSGDRTPRDLTTLAKDVV
                     KERLQTISGVGGVALVGGHEREILLALDLDRMETFGVAAADVRKAVAEANMEIPGGRI
                     DEHAREYLVRLAGRLPRVEDFEDVIVKSTGGAPVYLGDIARVIDTTVEQRSFSSFNRV
                     PAVSLTVTKQAGTNTVEMADLVRETVGDLNRELPSDVKVEIVEDVSTFIRDSVHEIQF
                     NILFGTLLAVIVIFLFLLDGRPTVITALAIPISLIATFAVMNFFGFTINIMTLLGLSL
                     AVGILVDDAIVVIENIYRHMAGGRTAFQAAISGTKEIGLAVMATTFSIVVVFLPVAFM
                     EGIVGRFFFEFGVTVAFAVLASLFVAFSLTPMLSARWLRAEAAQEEAAAKGRRVGARL
                     YDVLLYWNRAFDWLKPRYRTLLEKSLRARWVVVLVAAAAFVLAFVIPMIGLVGVEFMT
                     ETDQNQLAIDIETAPGTTLAETVERFQLVERTVRETLADQVTNTFITIGRGSDPVTRG
                     RLLVQLIDRSERELSARQLMDTVRAHVAGIPGLEASVSVGESKAGGNKSIEISVQGPE
                     HDEVVRLARRLQAYFYDVPGVVDVDNTLVEGKPEIAVTYDRRLASDLGISLQDIPMTV
                     RWLVEGDVVTRFKEGDEEYDVRMRLDERFRQSTENLGSILIKSDKSGSDGAPLLVPLE
                     RVAQLRKTAEISEYYRYNRQREIRVNANVTSTQFPGTATNLIMEKFSAEVGASGGYVV
                     APVGQQEIMEESFRNILRALFLAVIFIYLLLASQYESFWDPFSIMLSLPLSLVGAIIG
                     LIGSSFSIMSLIGIVLLMGLVTKNAILLIDFVKQERAKGVSRTDAILAAGPVRLRPIM
                     MTTLAMAFGMLPLALGIGPGAELRAPMARAVIGGITSSMLLTLVVVPVVYTLIDDAVG
                     LFRRKKAPGAVLKEDSTVRG"
     gene            16347..17957
                     /gene="bshC"
                     /locus_tag="PKM94_08285"
     CDS             16347..17957
                     /gene="bshC"
                     /locus_tag="PKM94_08285"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOD66676.1"
                     /translation="MTPGKALGYSELYLDFVSGRETARHFYTPDNLSAVAARLDGAVY
                     ARDRTADILRRQNQAWGAGEPTLAAIERLRDPRAVAVFAGQQAGLFGGPLLVQIKALG
                     IIQAARRIGRELGRPAVPIFWIAADDHDFAEINHTWVPDRAAVEPVKIVYPTAPAEAA
                     PAAEIRFADAEALAAAKARLREALGETEFTPALYGLIDRCYTPEETFVGAFAKLMTAL
                     LGEFGLVLFSPADPEAKALAAPLLQALAERHEEVRALVGRTNENLRSHGYHVQVEKKD
                     ESVHLFFARDGRRPVLRQSGGGFRIGDRAVTAADLRAMIAQEPERFSPDVLTRPVMQS
                     WLFPAAAQHGGPSEIAYFAQVTPLFELFGRPAPVYRARPSATFVERRYEAMMAQHRIT
                     LEELAGDIEQVVNRVTAESFPPDLEQAFGEARREVRERFEALAAQTLGFDPQLQSAAA
                     QAAGRIDFQIVQFEGKVFAAHKRRLGEVRERLYRLHRVLYPHRGLQERALNIGYFLAK
                     FGPAFVRFAHERLDSEMAAHQLIPLSDV"
     gene            17971..19101
                     /gene="bshA"
                     /locus_tag="PKM94_08290"
     CDS             17971..19101
                     /gene="bshA"
                     /locus_tag="PKM94_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOD66677.1"
                     /translation="MVIGITCYPVAGGSGIVATELGQQLALRGHQVHFISYAIPFRLD
                     RYQTNVLYHGVETTAYPLFKSPPYTLTLAAKMAEVTRSFGVDVLHMHYAIPHAASAYL
                     ARQMLLAGGGRAPKVITTLHGTDITLVGADPSFFDITRFSIMTSDGVTAVSRYLAAET
                     EEVFKLGKEIRVIHNFVDQKRFKPTTGDCARSEFARPEEFLVCHVSNFRPVKRTLDVI
                     DIFAKITAELPAKLVLIGEGPDTILARRQITKRDLGAKVIFLGNQSRVEAVLPCCDLF
                     LIPSEEESFGLAALEALACGVPVIGTSGTGLAEVVDDYRNGFLLPVGDTTSMARAAVS
                     LLRHPERLAEFRKAAIESATVKFGAERIVGEYERYYEEVLRG"
     gene            19094..19816
                     /gene="bshB1"
                     /locus_tag="PKM94_08295"
     CDS             19094..19816
                     /gene="bshB1"
                     /locus_tag="PKM94_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOD66678.1"
                     /translation="MAEAGRLEVLAIAAHPDDAEITCGGTLLKLAALGRATGVLDLAR
                     GEAGTYGTEADRDAEASAAGRVLGLAWRENAGMPDAAIEYTQANKLVIAQYIRDHRPE
                     LVILPHWEQRHPDHLAASRLGYDACFLAGLTKIDLAGKPHRPRKIIYASYFRNREFSF
                     LVDISAHFEKKCEAVGAYRSQFSDNNIARNIFHPGVTVFDLMRDRGANLGHMAGVRYA
                     EAFTIKEAILVDDPQTMPVQSI"
     gene            19862..20821
                     /locus_tag="PKM94_08300"
     CDS             19862..20821
                     /locus_tag="PKM94_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOD66679.1"
                     /translation="MYRTAMPETEKVGQAAVTATEAENLRAGLRVTWVGVLVSGALVA
                     FQVFAGIIANSQALIADATHSLSDLFGNVVVLFGLRWGRKVEDVDHHYGHGRIETMSG
                     MVVGLLLIAVAIGIVWNALATIYHHEVSRPGALALWAAVAGIVSKEALYWWTLAVGKR
                     LKSMAIIANAWHHRSDALSSVAVLIGISASYLNPAWHLADAIAALAVTYFIGRVGGKL
                     TWAAFRELSDAAPDREILLEIAERTRATAGVRQVHDLRARQSGAQIFVELHIVVDPTI
                     SVRDGHTIARQVKRMILRDFVDVTRVIIHVDPELKEDLGAMLS"
     gene            complement(20850..>21228)
                     /locus_tag="PKM94_08305"
     CDS             complement(20850..>21228)
                     /locus_tag="PKM94_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66680.1"
                     /translation="IVHVAWLAAGLPVLWAYTRFAVGREIISLGRGALTITHRPIPLG
                     FARRYRLANAHRFRVDTAGLRIANVRHRRNPLRWRGEQGMILFDYNLRPVRFGVGLST
                     AEARRLLQAIRDSGQVSPEQVEL"
BASE COUNT         3585 a         6385 c         7802 g         3456 t
ORIGIN      
        1 gagctcggag ccctgctggc cttctcggcg atcaagaaca acgacaaggt cggcctgatc
       61 atcttcaccg accagatcga gaagttcgtg ccgccgcgca aggggcgggg gcacgtcctc
      121 cggctgatcc gggagattct ctttttcgag ccgaagcgga cggggacgga cgtggcgggg
      181 gcgctcgagt acctcaaccg ggtggtgcga cggaagtcgg tggttttcct catctccgat
      241 ttcctctcgg aggattttca caggccgctc caggtggcca accggcggca cgatctgatc
      301 gcggtgaaga tctcggatcc gcgggagagc cggttcgacg atgtgggact gatcgagctg
      361 gaagacgccg agacggggga ggtctatgtc atcgacacgg ggtcgaagga gttccggcgg
      421 gagtacgccg cccgggcgga cgaggatctc ggccgcctgg agcgggagtt ccgcctgatc
      481 aatctcgatt tcatcaacat ccggaccgac cagccgtata tcaaaccgct gatcgcgttt
      541 ttccggagcc gggagcggag gctgtaggat ggcgggacgg cgggcgacag cgctgatgct
      601 cggactggcc tgggccgcgg cgatcgcggg gtgcgggggg tcgaccgccc acgaggcggc
      661 gatcgaacgc cacaagaaac tggccgccga actgcgcgac aaccggctct acgacgcggc
      721 ggtggaggag taccggcaga ttctggccta cgacgatctc gacgacggca cccgggcggg
      781 cgtcagttac ctcatcgggc ggctctattt cgagaatatc ggggactacg agcaggcggc
      841 agcctactac gtgcgcgcca gagcgctcga cccggacggc gatttcgtgc tggaggcgtc
      901 gaaaaatctc gtcacggcgc tggagcggtc gggtaatctc gtgaacgcca agcggcagct
      961 cgatgtactg accgatatcg gggcggcgcc ggccggggac tccgacgtag cggtggcccg
     1021 gatggggggc gtgcccgtgt ggcagtcgca ggtcgacgac gcaatggaaa tgctcccgcc
     1081 cgaagcccag aaagagtaca cgcgcgaccg cgaggcgcgg gtgcgcttca tgcgccagta
     1141 cgtggccgcg gagttgctct accacgcggc gatccgggag gggttcgaca aagatgcgga
     1201 gatcgcgcgg cggacgcggg aactgaaccg gcagttgctg gtggaaaagt atgtccggga
     1261 gaaggtggcg ccccagattc agatcgacac gctcgacctg cgcaacttct acctggcgaa
     1321 caaggacagc cgctacggcg gcgcgccgtt cgattcggtg caggcgcggg tgttgctcga
     1381 ctaccagtcg cagaaggcgg agtcggcctt ccaggactac ctcgccctgc tggcccaaaa
     1441 ggaacgggtg gagtttttgg accggaatgt caagtgagag gatgatgcgg acgagacggc
     1501 cataccggct cgcggcgatg gcggcggcgg cgctggcgct ggggccggcc gcgccggggg
     1561 cgttcggcca ggcggccgga gcggcgcccg gcgacacggt cgggtcgctg ccgggagtgg
     1621 aggtgcgcac ctcggtggac cgggcgaaga tctttgtcgg cgaccaggtg acctacacgc
     1681 tcacgatcat ccacgacagc acgattgacc tggtgccgcc gccgctcggg gccaacctcg
     1741 gggcgttcca ggtgctcgac tacgagaccg accgcgtgac ccggctcccc gacgggcggg
     1801 tgcagaacga gagccggttc gtgatcagca cgttcacgac cggggagtac gcggtgccgc
     1861 cgctgccggt catggcgatc atgcccgaca gcagccggaa agtgctgctg gccgaggcgg
     1921 tgccgatcac ggtggagtcg atcctggcgg cgggcgacac gctgatggac atcaagccgc
     1981 tcgcgggggt gctcgggccg gaggcgttcg cgaaggaccg gacgtggtgg tatgtcggcg
     2041 ggggagcggc ggcggcggcg ctgctggcgg cgctcctgtg ggcgctcctg cggcgcaagc
     2101 ggaagcccgc ggcggcggcg gtcgatctcc ggccgccgtg ggagatcgcc tccgagcggc
     2161 tcgccctcct gctccataaa aacctgatcg ccgacgggcg cttcaaggag tactactacg
     2221 aactgagcga caccctgagg tggtacctcg ggcgggtgta cgaccggaac gtgctcgact
     2281 ggacgacgga ggagttcctc acggcgtttc gggagatcga gctgccgcac tcgctctacg
     2341 acggggtgga ggcgttcatg tcgcacgccg acctggtgaa attcgccaag tacgagccgt
     2401 cgatcgagca gactaagtac gatttcgagt ttgtccacgc ggcggtcgac cgggtgcggc
     2461 aggacgtgtt ccggcggcag caggggcagg cggcggcggg gggcggccgg ccggcgcagc
     2521 cggtgggcgc ggccggggga gggacgcatg agtcttgagt tcgccggatt cagcgtcaac
     2581 ctgctggacc tgctggagac gacggtttcg ccgggagcga ttttcgcgac cgggggggca
     2641 atcgtgctgg ccatgcttgc gtactacttc acgcggcggc gggccgcggc ggcggcgatc
     2701 aagttctccg acctgcggat tgtcaagcgg gccggccgca cggggcggca gcggttccgc
     2761 ttcgtcctgc ctgtcctccg cgtgctcgcc gtggccctgt tggtggtggc gttcgcgcgc
     2821 ccgcggtcgg ggaccgagta caccgaggtg acatcggaag gaatcgacat catgatggcg
     2881 ctcgacgtgt cgtcctcgat gcaggcggag gacttcaagc cccacaaccg gctctacgtg
     2941 gccaaggagg agatgaagaa attcatcggg cggcgcatca acgaccgcat cgggctggtc
     3001 gtgttcgcgc gctactccta cacgcagtgc ccgctcacga ccgactacgg ggtgctcctg
     3061 agtttcctcg accagatcga tttcggactg gtggaggacg ggacggcgat cgggatggcg
     3121 atcgccaacg cggtcaaccg gctccgcgag agcgaggcca agagcaagat catcgtgctg
     3181 ctcaccgacg gcgacaacaa cgccggggag atcgatccgc tgacggccgc caatctcgcg
     3241 gccactttcg ggatcaagat ctacacgatc ggggcgggca agcccgggaa cgcgatgtac
     3301 ccggtggatg atccgctgtt cggaaagcgc tacgtgtacc ggccgacggt gatcgacgag
     3361 gagacgctcc gcgagatcgc cgcgcggacc aacgggaagt actaccgggc gcggtcgggg
     3421 gaggagctgg atcagatcta cgcggagatc gaccggctgg agaagacgaa agtggagacg
     3481 gcgcagcaca tccagtacgt ggaactgttc cagcatttcg cgttcggggc gttcggcctg
     3541 ctggtgctgg agatgctgct cgcccacacc tattttcgga aactgccgta ggagcgagag
     3601 agagtatgcg tttcgccgat ccgggcaatt tcgttctctt ggcgctggtg gcgctgctgg
     3661 cgctgttcgc ggggtttgcg ctggcgcgca agaagcgtct gctggcgcga ttcgccgata
     3721 tcccgctggt catgcgcaac gcgccctaca tctcgttcgc ccgccaggcc ggcaaggccg
     3781 ctctgctctt gctcgggatg gcgttcctca cgctggcgct gacgcggctg cagttcggga
     3841 cgcacctgga gatgctcaag cgggagggga tcgatctcgt ggtggcgctc gacgtgtcga
     3901 attccatgct ggcgcgggac atggcgccca accggctgga gaaggccaag caggaactcc
     3961 aggggatcat cgggcggctc aagggggacc ggatcggcct ggtggcgttc gccggcgagg
     4021 cctacatcca gtgcccgctc acgctggact acacagcggc cgagttcctg ctcggggcga
     4081 tggatcagaa ctcggtgagc gtgcagggga cctcgatcgg gtcggcgatc gacatggccg
     4141 cccggtcgtt cgagcagaaa gagcggcagc acaaggttct gctcctgctg acggacgggg
     4201 aggagcacga ccggggggcg gaggcggccg ccgaggcggc ccggcgcgag gggatcaaga
     4261 tttacacggt ggggatcggg aacccggccg gggaaccgat cccgctgttc gaccgccggg
     4321 gcgcgcaggt ggggttcaag aaggacgaca acggcgaggt gatcatcacc cggctcaacg
     4381 aggaactgct gcagaagatc gcgctggcga cgggcggcaa gtactaccac gcgacggccg
     4441 gggagatgga gctcgacagg attttcggcg agatatccga gatggagaag aaggagctgg
     4501 aggggacgct cgtgacgcgc tacgacgacc gcttccagtg gccgctcctg gcggccctgg
     4561 cgctggtggt gggggagttc tttgtctcgg aacggcggcg cgtgccgcga aaggagcatc
     4621 atgcgtaggc tggtggcgat cgtgctgctc gcggcgctgg cgggaccggc ccgggcggat
     4681 tttctcgacg agatccgcga cgggagccgg gcgtacgcgg cgggggacta caagaaggcg
     4741 atggagcatt tccacaacgc cgaggcggac cgccccgagg atccgcgcgt gcagtacaac
     4801 atcggcggaa ccgcctacga aatgggggac tacgagatgg ccgaggagta tctctccaag
     4861 gcgctcgcgg ccgccgatgc cgcgctcgag gccaaagctc actacaacct cggcaacacc
     4921 tattaccgga tgcaggatta ccccaaagcg atccagtcgt acgagaacgc gctcaagctc
     4981 gacccgaacg acaccaacgc caagatcaac ctcgaactcg cgcggcggat gctcaaggaa
     5041 cacaccaaac ccgaacagca gccgaaccag cagcagaacc agccgcaacc gcagcaggac
     5101 caacaacagc agcagcaaca gcaacagccg caggaccagg cgcagcccga ccagcagcag
     5161 gaacagcagc agcagcagac gggggaccag cagaccgaag agcagccgca ggaggggcag
     5221 aaggagcagc agcagaatca gcaggagatg aaggagcagc aggtctcgaa ggaggacgcg
     5281 gagcggatac tcaacgcgct gcgcgacgac gagcgggaga tccaggaacg gctcaagcgc
     5341 cgggtgcagg cgcgggggaa ctaccacgga aaggactggt gaggggatgg cgaggatgcg
     5401 gcgagggtgc ggattggggc cggcggcggc gctggcgatc ctgctgtggg ccgccggggc
     5461 ggcggcgcag gggacggggg aggtgcgggt ggaggtgtcg ctgaaaccgg attcgatcgg
     5521 cctcgacgag caggcgctgc tggcggtcac ggtgtcgggg gccgctcaga acctccccgc
     5581 cccggacatg ccctcgctgc cccggttcga ggtgtactcg cagggatcct cgagcaatat
     5641 cacgattgtg aacgggaaag tgagcgcctc gatgacctac cgctacctcc tcgtgccgac
     5701 caaggcgggg gtctacccga tcgagcggat cgccgtcgtc tacaacaacc ggcgctacga
     5761 ggggaacagg gtgacgctga cggtgctgga caaggggacg gcgatgacgc cggagatccg
     5821 gcgccagacg gaggagtcgg gggagggggc gaaggattac ttcctggagg cggtggtcga
     5881 caagaagcag ccctacgtga acgagcaggt gacgctcacg ctgaagttcc acatcgcggt
     5941 gcagtactac gggtcgccgg agctgatcga gccgaccacg accgggttct ggaccgaggt
     6001 cctggggaac gaagccccct accgccagca gatcggcggg cgggcctacc gcgtgatcga
     6061 gcgcaagtac gcgctctttc ccacccagac gggggagctg acgatcgggc aggcggcgat
     6121 ccgggtgacc gtgcccgagc gccggcagtc cacgcgcgac ccgttcggaa tgttcaacga
     6181 cttcttcagc tccgggcgcg aggtgacggt gcggagcaag ccggtgacgg tgaatgtgcg
     6241 gccgctgccg acggcggggc ggccggacga tttcaccggg tcgatcggca aatacgagct
     6301 gacggcgcgg gccgacaaga acgaggtcga ggtcaaccag ccggtgacgg tgacgttcca
     6361 ggtcagcggg acggggaaca tcaaggcggc ggccaaaccg ccgatccccc agacggacga
     6421 tttccgggtg tacgaggcct cgagcagcga gaatgtcggc aaacaggggg accgcctggc
     6481 ggggaccaag atctatgaag aggtgttcat cccgcggcgg ccgggaatgc tcgagatccc
     6541 ggcgatcacc tacagctatt tcgacccgga gcggggagcc tatgcgacga cctcgacgaa
     6601 accgatcgcc atccaggcgc tcaagccgga ggggtatgtc gcctccggcg atctccccta
     6661 ccagggcggc ggggtggtgg taggcagcga ggcgcgggac attcggtata tcaaggagga
     6721 tatcgggcgg cccgcaagaa gcgggcggat cattctgttg acgccgctgt acctgctggc
     6781 gaacggggtg ccggtgctgg tgctggcggc gctcatcgcg gtgcggaagc ggcgggagaa
     6841 gctggcggcc aacgtggggc tggcgcggtc gctgcgggcg gggaagatgg cccgcaagcg
     6901 gctggcgaaa gcgcgcgggc tagcaacggc ggcccagggg caggcgttct acgccgagct
     6961 ccaccagacg ctcaccgcct acgtcgccga caaactcaac gtctcccccc acgggctgac
     7021 gactgagcgg ctccgggagc tgctcgggca acagggggcg gacgaggggc tggtggcggc
     7081 ggtggccgat attctccagc ggtgcgattt cgcccgcttc gcgccggcct cgatctcgca
     7141 ggaagaaatc acgcgcgcgc tggccgacgt agaagagacg atgattcgtc tggagggtat
     7201 ccattttgcg taagctggtt ctcatgatcg ggctggcggc gatgctcgcg gcgccggccg
     7261 gggcggccga ggtggcggag gctttccggc aggcgaacgc gcggtacgag gcgcgggatt
     7321 tcgccggggc ggccggggcc tacgaggaca tcctgaagtc cggcacggcc tcggcccagc
     7381 tgtactacaa tctcgggaac gcctatttca agcaggggga tgtcgggcgg gcggtgctga
     7441 actatttgcg ggcgcagcgg ctcgacccgc gggacgagga catccggcac aacctggagt
     7501 ttgcccggca gttcgcgcgg gtgaagatgg agggcgtgga actgaacccg atcagcagtt
     7561 tcttcgcttc gctcgtgggg ccggtgcggc tgaaccttct ggcctggacc tcctcggccc
     7621 tgtttgccct cttctgcgcc atgctcgcga tccgctacgg gctggggttc cgaggggggt
     7681 gggtgcggct cggggtggtg gcggcgctcg tgctggcgac ggcggccgcg gggctgacca
     7741 cctacaagta ccgcaccgag tatctcaccc ggcgcgtggt ggtcgtggct gacgagagcc
     7801 cggtctacaa cgggccgacg gccaacgccg acgtcgagtt tcagggagcg ccggggttgg
     7861 tggcggaggt gctcggcgag agcggcgcct acctcaacgt gctgttcgaa aacaagcgcc
     7921 gggggtgggt gcgccgggac ctggtggccg aagtctgagc gaggggcggg cgggtttatt
     7981 tcgttgacac ccttggagaa gtcgtctatt atacctgccg cgacttcacc actggacaga
     8041 tcgggtgtcg atgatatgga agaagaaaaa gttctggggt acgctcgttg ccgtcgccct
     8101 gctcgccttc tgcgtcaagg atatcagcct caccgatctg cgcagcctct ccgtgcgtat
     8161 tgactacatc tacctgatcc cggcgacggt ctgcaccttc ctgtactcgg tgttcaaggc
     8221 gctccgctgg cgcctgatgg tcaagaccca gaagacgatc ccggcctgga acgcgatcac
     8281 gctgtattcg gcagggcagg tgatcaacgt ggtgatgccg gccctcacgg gacagctcgg
     8341 gcggatcctc ctgtttgcgc gggccgaggg gctgcgcaag acgttcgtgt tttcgacgat
     8401 cgtgctggag gtgctgctcg acgcgatcac gctggtggcg gcgctcctgt tgacctcgct
     8461 ggcgttcgcg attcccgacc ggtaccggac ggccgggtac atcatcgggc tggtgacgct
     8521 ggtgctgatc gggctgctct acctcctcct gcactacgag gaccgggcgg agaatttcgg
     8581 cggggcgtgg tgccgggcgc ggtggccgaa attccacgcc gggatgcaga agtcgatccg
     8641 gtcgttcacg agcgggatgg agctgctccg ctcgagccag aacgtctgtc tctcgctcgg
     8701 gctgtccttt ctgtactggg gggcgcacat ctttgtcgtc tatttcctct tcctcgcgtt
     8761 cgggttcggg ctgccgtttt cggcggcggc gatggtgatg gtcatcaaca ccctggcgct
     8821 catggtgccg atcacgccgg ggaacgcggg gacgttcgag gtggcggtga gcacggcgct
     8881 cctggcgttc cgggtggcgc ggccggatgc ggtgctcttc gccctggccc tccacctgct
     8941 cgatctgctc ccgctctggc tgctcgggat gtggttcttg cggacggaga aggtgtcgct
     9001 gaaggatatc cgcgaacagc acgaggaccg gctgattctc gacgagatgg acgagcgcgg
     9061 gtcgctcatc gagcgggagg atccgcgatg atccggtcgt ttctctatct gcccggcgag
     9121 ggcgtcgaca cccgggaggg gatcgccgat tttcgggaga tgaccgagaa ggagggggcg
     9181 atcctgtggg tggacctgtg caagccgagc gacgaggagt cgtacgtgct cacccacgat
     9241 ttcaatttcc accccctggc gatcgaggac gtgatctccg aaaagccgcg gaccaagctc
     9301 gacgactacg accgctacct gttcctcgtg ttccagattg tcgactacgt ggggcgggag
     9361 gaggggctga agatcgccga gctcgacctg ttcctcaccc agaacgcgct ggtcacggtg
     9421 cactacgacg accaccggat ttacgactac ctctaccacc gggcggagcg ggacgagcgg
     9481 ctgatgtcgc ggggggtgga tttcctgttc cacgcggtga tcgacgcggt ggtcgacaac
     9541 tacaacgcga ccctcgacat cctcgagtac gagatcgaca agatcgagga cgatgtgctg
     9601 gaggatctcg accaggagac gctccggtcg attttcaccc tccgccgcga catcatccag
     9661 ctccgccgga tcgtgatccc gcagaaggag gtggtgctcc acctgtcgcg ggagcagttc
     9721 ccgcagattt cgacgcgggc ggcggtgtac ctgtcggaca ttcacgacca cctcgtgcga
     9781 atcgacgacc tggcgaattt ccaccgcgag atcctcaact cgtcgctcga aatctactac
     9841 tcctcgattt cgacgcgcac caacgaggcg atcaaggtcc tgaccatcct cagcgtgctg
     9901 ttcattccgc cgaccttcct cgtgggcgtg tgggggatga atttccggaa catgcccgag
     9961 ctcgagtggc gctacgggta cggggcggcg ctcctgttca ttttcctgat catcggcggg
    10021 ctgctcgtct atttccgcaa gaaaaagtgg atttagcgga aaattgtttg aaacttccct
    10081 tgacaacggg ccgttcatcc cctatagata ccgaccggta ggtatgtaga gatggcgaac
    10141 cgacaggtga aaaagcgcat ccgccagagc tcgaagcggc cggccggcga gcgccgggac
    10201 cagttgctcc gggcggccca cgagctgttc ctccggcagg gctaccgggc gacgagcacg
    10261 gacgagatcg cccgccgggc gggcctcacg aagggggcgt tctacttcca cttcgccagc
    10321 aagaaagaca tcctcatgac tttcgtccag gacttcgtcg aggggcacac gcgggcgatg
    10381 gagcgcctgg cggggaagcg gctgacgccg gcgggtcttt tggccgagct ggggcggatc
    10441 gacgcgcgca tgccgctgcg ggaggcgcgc aagaatctct cgctgttggc ggaaatcatg
    10501 tacctgccgc gctttcgcgc gctgatccgc cgggcttacg agcggtcggt ggcggtcatg
    10561 gcggagtgtc tcgacccggg ctacgggcgg acggccgccc agcggcggcg gctggtcgag
    10621 ctcatgcacg cgatctacga cggcgtgtgt ttcgccggca tggtgcatcc gcagctcggc
    10681 aatttcgaac gacaactgga aacattcagg cggctgacgg ccaagaagag gatacggagt
    10741 aatcgaaagc atggtcccgc gaaaagttga gaaaccgggc cgggcggccg ggccggtgcg
    10801 gcggtggggc ggggtggtgg cgctggcggc ggctctggcg gcggccggcg ccgcggcggc
    10861 ggccgatggg gcgcgcccgg acacgatggt gctgacggcg gcggaggtcc gccgcctggc
    10921 gcttgagtac aaccgcgatt acctgcaggc gctcgaggag gtgcgggcgg ccgatgccga
    10981 ggtggtgaag gcgcgggcgg gagccctgcc ggacatctcc ggtcaggcct actacgaccg
    11041 caatttcctg atcgccagca atttcgtgca gatgggcgac gaaacggtgg agttccggtt
    11101 cgggttcaag aactcgttcg gggccggcat ctcgctgcgc cagccgatct accagggggg
    11161 caaggtgttc acggccgtgt cgatcgccca gcagtaccgc aagtacgcgc gggccggcgc
    11221 cgccgaggcc gccgccgaag tggcctacac ggccgaggtc ctgttctacc aggtcggcct
    11281 ccgggagtcg caggtaaacg tcctccggca ggcgctcgaa gcggccgagt tgaatgcgga
    11341 catggtccag aagcagttcg accaggggat gacctcggag ttcgaggtgc tccgggcgcg
    11401 ggtggagcgc aacaacctcc tgccgcagct gatcgcctcc gagtcggagg tggagctgtc
    11461 gcggaagcgg ctgaagtcat tcctcgggct ggatttgtcg cggccgattc ggctggaaga
    11521 gccgggggcc gatgcggata cgagtctggc ggggctgggg ccggaaccgg cctatgtcga
    11581 cacggcgctg gcgcggcggc cggcgatgca gcaggcggac ctgctggtgg atatcgcgaa
    11641 gaaggcggtc aaaatcgccc aggccgacta ctggccgaat ttcgaggccg tgtcgagctg
    11701 gggctggtcg gcggtgtcgg acgatttcac gctccgcgaa aacaacagcc ggtcactcac
    11761 ggccgggttg atggtgtcga tgccgatttt cgacggcctg cggcggtcgg ggcaggtgac
    11821 gcaggcgaag gtggaccaca accaggcggc gctggcggcc agcaagatcc gcgacgacat
    11881 ccggctggaa gtcgagcagt cctacgatgc gctcgtgcag gcgaagaagg cgctcgacat
    11941 ccaggggacg aacatcgcgc aggcggagga ggggctgaaa atcgccaacg tgcggtatga
    12001 gtcgggggtg gggacgctct tggaggtgct ctcggcgcag gtggcgctga ccgacgcccg
    12061 cagtgcgcgg gtgcaggcgc tctttgcgtt ccgggtggcc caggcgaagc tgaaacgggc
    12121 aagtatgatc caaccgagta tggaataagt gtatgcagat gcgacgaatg attctggccg
    12181 gcggcgcggc ggccgccgcg atcctggccg gctgcggcgg cgcccagcag gatacggccc
    12241 agcggccgga ggtaccggtc cgcgtgcagg tgctggcccc ctttgacgga gcggtgacga
    12301 agacctatac cggcgccctg gagggacaga accaggcagc catctacacc aaaatcgccg
    12361 aggcggtgga gagcgtgctc gtccgcgaag gggggcgagt gcaggccggg caggtgctgg
    12421 tcaagctcga ccggaccggg tcgatgtcca actaccagca ggcgctcagc gtctaccgga
    12481 acgccgagaa aaattacacg aaaatgaaga cgctctacgg gcagggggcg gtgagcgaga
    12541 tgcagtatga tgcggcgcgg acggagtacg aggtggcgca ggcctcgttc gaggcggccc
    12601 ggcggctggt ggaaatccag tcgcccatct cgggcatcgt gacctcggtc aatgtccggc
    12661 gcggcgacta cctcgcccag gggcagatgg tggcgacggt ggccgcgacc gaacggctgc
    12721 gggtccgttt ccaggtcaag gccaccgacg tcggtctggt ccaggtcggc gacacggtcc
    12781 gcgtgacggc cgagaccgcg gcggagtcgc tgcccggcgt ggtggcggcg gtggcgcagt
    12841 cggccgaccc ggcctcccgc gccttcgagg tcgaggctct cgtggccaac gccggagaaa
    12901 gctcgttccg gccgggcatg tttgtcaggg tggaactggt catggagcgt ctgcagggcg
    12961 tgctcggcgt cccgccgagc gcggtcgtgg cgctgagcag cggcgcggtc gtctacgtag
    13021 tcgaaaacgg cctcgcccgt cggcggccgg tgacgctggg gacggaagcc ggagggcggg
    13081 tgatcgtgag cgagggactg cggccgggag acagcgtgat cgtcctcggc cagagctacc
    13141 tggacgacag cgccgcgatc cgcgtcaccg atgtggagaa gggcgcccga tgaaactctc
    13201 cgaagttgct gtcggccggc cggtcttcac gaccatgatg atggcggcgc tgctggtgct
    13261 ggggctggcc tcgtacaccg agctcccgat cgatctgatg cccgacatcg agttccccta
    13321 ctccgtgatc cagacggtct acccgggggc ctcggcggag acggtggaga gcgaagtcac
    13381 ctcgccgctg gaagacgagg tgaacctgat ctcgggcatc cgccacatcg agtcgcgctc
    13441 gctggagggc tactccttgg tcatcatcga attcgagttg gagcgggatg ccgccgaggc
    13501 cacccaggag gtgcgggaga aggtggccgg gatccgcagc gatctgccgg acgacatcga
    13561 agaaccgatg gtgctgaatt tcgatcccac ggcgtttccg atcatgtcgt tggctgtgtc
    13621 gggagaccgc accccgcggg acctgacgac gctggcgaaa gacgtggtga aggagcggct
    13681 gcagacgatt tccggggtcg ggggggtcgc gctcgtcggg gggcacgagc gggagatcct
    13741 gctggccctt gacctggacc gtatggagac attcggggtg gcggcggccg acgtgcgcaa
    13801 ggcggtggcc gaggccaaca tggaaattcc gggcggccgg atcgacgagc acgcccggga
    13861 gtacctggtg cggctggccg gccggctccc gcgggtcgag gatttcgagg atgtcatcgt
    13921 caagagcacg ggcggcgcgc cggtctacct cggcgacatc gcgcgggtga tcgacaccac
    13981 ggtcgaacag cggtcgttct ccagcttcaa ccgcgtcccg gccgtgtcgc tgacggtcac
    14041 caagcaggcg ggcacaaaca ccgtggagat ggccgatctc gtgcgcgaga cggtgggcga
    14101 cctgaaccgg gagctaccct ccgacgtcaa ggtggagatt gtcgaggacg tgtcgacgtt
    14161 tatccgcgac tcggtccacg agatccagtt caacatcctg ttcggcacgt tgctggcggt
    14221 gatcgtgatc ttcctgttcc tgctggacgg ccggccgacg gtgatcacgg cgctggcgat
    14281 cccgatttcg ctgatcgcga cgtttgcggt catgaacttt ttcgggttca cgattaacat
    14341 catgaccctg ctggggctgt cgctggcggt ggggatcctg gtggacgatg ccatcgtggt
    14401 gatcgagaac atctaccgcc acatggcggg agggcggacc gcctttcagg cggccatctc
    14461 gggcaccaag gagatcgggc tggccgtcat ggcgacgacg ttctcgatcg tggtcgtgtt
    14521 cctcccagtg gccttcatgg aggggattgt cgggcgcttc ttcttcgagt tcggcgtgac
    14581 ggtggcgttc gcggtgctgg cctcgctgtt tgtcgcgttc agtctcaccc cgatgctgtc
    14641 ggcgcggtgg ctgcgcgccg aggcggccca ggaagaggcg gccgccaagg gccggcgtgt
    14701 cggcgcccgg ctgtatgacg tgctcctcta ctggaaccgc gccttcgact ggctgaagcc
    14761 gcgctaccgg acgctcctgg agaagtcgct gcgcgcccgc tgggtggtgg tgctggtggc
    14821 ggcggctgct ttcgtcctgg ccttcgtcat ccccatgatc gggctggtcg gggtggagtt
    14881 catgaccgag accgaccaga accaattggc gattgacatc gagacggctc cggggacgac
    14941 gctggcggaa acggtggagc gcttccaact ggtcgagcgg acggtgcggg agacgctggc
    15001 cgaccaggtg acaaacacgt tcatcacgat cgggcggggg agcgatccgg tgacgcgggg
    15061 gcggctgctc gtgcagctca tcgaccgcag cgagcgcgag ctgtcggcgc gccagctcat
    15121 ggacacggtg cgcgcgcacg tcgcgggcat cccgggcctg gaggcttcgg tcagtgtcgg
    15181 ggagagcaaa gcgggcggca acaagtcgat tgagatttcc gtccaggggc ccgagcacga
    15241 cgaggtcgtg cggctcgccc gtcgcctcca ggcctacttc tacgacgtgc cgggggtggt
    15301 cgacgttgac aacacgctcg tcgagggaaa acccgaaatc gccgtcacct acgaccgccg
    15361 cctcgccagc gacctcggga tctcgctcca ggatattccg atgacggtcc ggtggctggt
    15421 cgagggggac gtggtcacgc ggtttaagga gggggacgag gagtacgacg tccgcatgcg
    15481 gctggacgag cggttccggc agtcgacgga gaatctcggc agcatcctca tcaagagcga
    15541 caagagcggg tccgacgggg cgccgctgct ggtcccgctc gagcgcgtgg cccaactgcg
    15601 gaagacggcg gaaatcagcg agtactaccg ctacaaccgg cagcgggaga tccgggtcaa
    15661 cgccaacgtg acgagtacgc agttcccggg gacggcgacc aacctgatca tggagaaatt
    15721 ctccgccgag gtgggggcgt cgggcgggta cgtggtggcg ccggtgggcc agcaggagat
    15781 catggaggag tcgttccgca acatcctgcg ggcgctgttt ctcgcggtca tcttcatcta
    15841 cctgctcctc gcctcgcagt acgagtcgtt ctgggatccg ttctcgatca tgctgtctct
    15901 gccgctctcg ctggtggggg cgatcatcgg gctgatcgga tcgtcgtttt cgatcatgtc
    15961 gctcatcggg atcgtgctcc tgatggggct ggtgacgaag aacgcgattc tgctgattga
    16021 ttttgtgaaa caagagcggg cgaaaggtgt ttcaaggacg gatgcgatcc tggcggccgg
    16081 gccggtccgg ttgcggccga tcatgatgac gacgctggcg atggcgttcg ggatgttgcc
    16141 gctggcgctc gggattggtc cgggcgcgga actgcgcgcc ccgatggcgc gtgcggtcat
    16201 cggcggcatt acctcgtcga tgctgctcac gctcgtggtg gtgccggtgg tgtacacgct
    16261 gatcgacgat gctgtcggcc tgttccggcg caaaaaggcg ccgggggcgg tgctgaagga
    16321 ggattcgact gtccgcggtt agactggtga cgccgggcaa ggcgctcggc tacagcgaac
    16381 tgtatcttga tttcgtgtcg ggccgggaaa cggcccggca cttttatacg cccgacaacc
    16441 tgtcggccgt ggccgcgcgg ctcgacggcg ccgtctatgc gcgcgaccgg acggcggaca
    16501 tcctgcggcg gcagaaccag gcctgggggg cgggcgagcc gacgctggcg gcgatcgagc
    16561 ggctgcgcga cccgcgggcg gtggcggtgt tcgcggggca gcaggcgggg ctgttcggcg
    16621 ggccgctgct ggtgcagatc aaggcgctcg ggatcatcca ggcggcgcgg cggatcggcc
    16681 gcgagctcgg gcggcccgcc gtgccgattt tctggattgc cgccgacgac cacgatttcg
    16741 cggaaatcaa ccacacctgg gtgccggacc gggccgcggt cgagcccgtg aagatcgtct
    16801 acccgacggc tccggcggag gcggcgccgg cggcggagat ccgttttgcc gacgccgagg
    16861 cgctcgcggc ggccaaagcg cgcctgcgcg aagcactcgg ggagacggaa ttcaccccgg
    16921 ccctctacgg gctgatcgac cgctgctaca ctccggagga gacctttgtc ggcgcctttg
    16981 cgaaactgat gactgcgctc ctgggggaat tcgggctcgt cctgttctcg cccgcggatc
    17041 cggaggccaa ggcgctcgcg gcgccgctcc ttcaggcgct cgcggagcgg cacgaggaag
    17101 tgcgcgccct ggtcggccgg accaacgaga acctccgcag ccacgggtac cacgtccagg
    17161 tggagaagaa ggacgagtct gtgcacctgt ttttcgcgcg cgacggccgg cggccggtgc
    17221 tgcggcagag cggcggcggc ttccgcatcg gggaccgggc ggtcacggca gcggatctgc
    17281 gcgccatgat cgcgcaggag ccggagcggt tctcgcccga cgtgttgacg cggccggtga
    17341 tgcagtcgtg gctgtttccc gcggcggccc agcacggggg accctcggag atcgcctatt
    17401 tcgcgcaggt gacgccgctg ttcgagctgt tcgggcggcc ggcgccggtg taccgcgcgc
    17461 ggccttcggc cacattcgtc gagcgccgct acgaagcgat gatggcgcag caccggatca
    17521 cgctggagga gctggccgga gatatcgagc aggtcgtcaa ccgggtgacg gcggagtcgt
    17581 ttccccccga tctcgaacag gcgttcgggg aggcgcggcg ggaggtccgg gagcggttcg
    17641 aggcgctggc ggcgcagacg ctcgggttcg atccgcagct tcagagcgcg gccgcgcagg
    17701 cggccggcag gatcgatttc cagatcgtgc agttcgaggg caaggtgttc gccgcgcaca
    17761 aacggcggct cggggaggtg cgcgagcgcc tctaccgcct ccaccgggtg ttgtacccgc
    17821 accggggcct gcaggagcgg gcgctgaata ttggatattt tttggccaag ttcggcccgg
    17881 cctttgtccg gttcgcgcac gaacggcttg acagcgagat ggcggcgcac cagcttattc
    17941 cgctgtcgga tgtataggag cccaacagat atggttatcg gcatcacgtg ttacccggtg
    18001 gccggagggt ccggcatcgt ggccaccgag ctcgggcagc agttggcgct gcgcgggcac
    18061 caggtccact tcatctccta cgccatcccc ttccggctcg accggtacca gacgaacgtg
    18121 ctgtaccacg gggtggagac gaccgcctat ccgctgttca agtcgccgcc ctatacgctc
    18181 acgctggcgg cgaagatggc ggaggtgaca cggagtttcg gcgtggacgt gctccacatg
    18241 cactacgcca tcccgcacgc ggcctcggcc tacctggccc ggcagatgct cctggcgggg
    18301 ggcgggcggg cgcccaaggt gatcacgacg ctccacggca cggacatcac gttggtcggc
    18361 gccgacccgt cgttcttcga catcacccgg ttttcgatca tgacctcgga cggcgtgacg
    18421 gcggtgtcgc gctacctggc ggccgagacc gaggaggtgt tcaagctcgg caaggagatc
    18481 agggtgattc acaacttcgt cgaccagaag cggttcaagc cgacgacggg ggactgcgca
    18541 cggtcggagt ttgcccggcc ggaagagttt ctcgtgtgcc acgtctccaa tttccgtccg
    18601 gtgaaacgga ccctcgacgt gatcgacatt ttcgccaaga tcacggccga gctgccggcc
    18661 aagctcgtgc tgatcggcga ggggcccgac acgatcctcg cgcgccgcca gattaccaag
    18721 cgggacctcg gggcgaaagt gattttcctg ggaaaccaga gccgggtcga ggcggtgctg
    18781 ccctgctgcg atctgttcct gatcccctcg gaggaggagt cgttcgggct ggccgcgctg
    18841 gaggcattgg cctgtggcgt gccggtgatc ggcacctccg ggaccggcct ggccgaggtg
    18901 gtggatgatt accgcaacgg gttcctgctg ccggtcgggg atacgacctc gatggcgcgg
    18961 gcggcggtgt cgctcctgcg ccaccccgaa cggctggccg agttccgcaa ggccgcgatc
    19021 gagagcgcga cggtcaagtt cggcgcggag cggattgtcg gcgaatacga acgctactac
    19081 gaggaggtgc tccgtggctg aggcggggcg gctggaggtg ctggcgatcg cggcccaccc
    19141 ggacgacgcc gagatcacct gcggcgggac gctgctgaag ctggctgcgc tggggcgggc
    19201 gaccggggtg ctcgacctgg cgcgggggga ggccgggacc tacgggaccg aggcggaccg
    19261 ggacgcggaa gcatccgcgg ccgggcgcgt gctcgggctg gcgtggcggg agaacgcggg
    19321 gatgccggac gcggcgattg aatacactca ggccaacaag ctggtcattg cccagtacat
    19381 ccgggaccac cgtcccgaac tggtcattct cccccactgg gaacagcggc acccggacca
    19441 cctggcggcc agccggctcg gctacgacgc ctgttttctg gccgggttga caaagatcga
    19501 cctcgccggg aaaccgcatc ggccgcggaa aatcatctat gcctcctatt tccggaaccg
    19561 cgagttctcc tttctcgtcg acatctccgc gcacttcgag aagaagtgcg aggcggtggg
    19621 ggcctaccgt tcccagtttt cggacaacaa catcgcccgg aatatcttcc acccgggcgt
    19681 gaccgtgttc gacctcatgc gcgaccgcgg ggcgaatctc ggccacatgg cgggggtgag
    19741 gtacgcggag gcgttcacaa tcaaggaagc gatcctggtc gacgatcccc agacgatgcc
    19801 ggtgcagtcg atctaggcgc cgggcgggcg cggatggtgc ttgacggccg gggcggccgc
    19861 gatgtatcgt accgccatgc ccgaaacgga gaaggtcggt caggctgcgg tgacggcgac
    19921 ggaggcggag aatctccgcg ccgggctgcg cgtaacctgg gtcggggtgc tggtgagcgg
    19981 cgccctggtg gcgttccagg ttttcgccgg aatcatcgcc aattcccaag cgctcattgc
    20041 cgacgccacc cactcgctgt cggacctgtt cggcaatgtc gtcgtcctgt tcgggctgcg
    20101 gtgggggcgc aaggtcgagg atgtcgacca ccactacggc cacgggcgga tcgagacgat
    20161 gtccggcatg gtggtggggc tgctgctcat tgcggtcgcg atcgggattg tctggaatgc
    20221 gctcgccacg atctaccatc acgaggtgtc gcggccgggg gcgctggcgc tctgggcggc
    20281 ggtggccggg atcgtgtcca aagaagcgct ctactggtgg acgctggcgg tcggcaagcg
    20341 gctcaagagc atggcgatca tcgccaacgc ctggcatcac cggagcgacg cgctgagttc
    20401 ggtggcggtc ctgatcggga tctcggcctc ctacctgaat cccgcgtggc atctcgccga
    20461 cgcgatcgcg gccctcgcgg tcacctattt catcgggcgg gtcgggggga aactcacctg
    20521 ggcggctttc cgcgagctct ccgacgcggc acccgaccgg gagatcctgc tggaaatcgc
    20581 ggagcggacg cgcgccaccg ccggggtgcg gcaggtgcac gacctccggg cgcggcagtc
    20641 gggggcgcag atctttgtcg aactccacat cgtcgtcgac ccgacgatca gcgtgcgcga
    20701 cggccacacg atcgcccgcc aggtcaagcg gatgatcctg cgggattttg tcgatgtcac
    20761 gcgcgtgatc atccacgtcg atccggagct gaaagaggat ctgggggcga tgctgtctta
    20821 gggcggcgcc ggggcatagg gggagcggcc tacaactcca cctgctccgg ggaaacctgg
    20881 ccgctgtcgc ggatcgcttg gagcaggcgc cgggcctcgg cggtgctcag gccgacgccg
    20941 aagcgcacgg gtctcagatt gtaatcgaac aggatcatcc cctgctcgcc gcgccagcgg
    21001 agcgggttcc ggcggtgccg cacgttggcg atgcggagac cagcggtgtc gacgcggaag
    21061 cggtgggcgt tggccagccg gtagcggcgg gcaaagccca gggggatggg gcggtgggtg
    21121 atggtcagcg cgccgcggcc gagggagatg atctcgcggc cgacggcgaa gcgggtgtag
    21181 gcccacagca ccggcagacc ggcggcgagc caggcgacat ggacgatc
//
LOCUS       DAOPOE010000053        21150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3099_length_21150_cov_5.113629, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000053 DAOPOE010000000
VERSION     DAOPOE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21150
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_21150_cov_5.113629"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="map"
                     /locus_tag="PKM94_08310"
     CDS             <1..663
                     /gene="map"
                     /locus_tag="PKM94_08310"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOD66681.1"
                     /translation="TVIRPGMTTGQLDRLIEDFIRAEGAIPAFKGYQGFPASACISID
                     DEVVHGIPGKRVIEEGVIVSVDIGAIVDDYYGDSARTFGIGEVSEEKRRLMEWTLKAL
                     HAGIDKARKGNRLGAVSAAIQQVAESQGYGVVRQLVGHGIGRAMHEEPQVPNFGSPAD
                     GPVLEAGMVIAIEPMINMGTYEVRTLPDGWTVVTADGRPSAHFEHTVAVGPDGPRILT
                     AA"
     gene            672..890
                     /gene="infA"
                     /locus_tag="PKM94_08315"
     CDS             672..890
                     /gene="infA"
                     /locus_tag="PKM94_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD66682.1"
                     /translation="MAKEETITVEGRVIEPLPNATFKVELDNGHVVLAHISGKMRMHF
                     IKILPGDKVTLELSPYDLTRGRITYRYK"
     gene            919..1035
                     /gene="rpmJ"
                     /locus_tag="PKM94_08320"
     CDS             919..1035
                     /gene="rpmJ"
                     /locus_tag="PKM94_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOD66683.1"
                     /translation="MKVKSSVKKRCEYCKIIRRHGVIRVICKRDPSHKQRQG"
     gene            1074..1460
                     /gene="rpsM"
                     /locus_tag="PKM94_08325"
     CDS             1074..1460
                     /gene="rpsM"
                     /locus_tag="PKM94_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOD66684.1"
                     /translation="MARIAGVDLPKDKRIEVGLTYIYGIGPHRARLILAKTGVNPDTR
                     VNQLTDEDVTKLRQVIENEYSVEGSLRGEIQMNIKRLIDIGSYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRRSIGGLKKKPVGKK"
     gene            1482..1877
                     /gene="rpsK"
                     /locus_tag="PKM94_08330"
     CDS             1482..1877
                     /gene="rpsK"
                     /locus_tag="PKM94_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOD66685.1"
                     /translation="MAEPKKKGRPRKKSRKVDTNGVAHIKATFNNTIITLTDAQGRAI
                     SWGTAGKVGFKGSKKSTPFAAQQAAASAVREAMDQGLRRVEVWVKGPGSGREAAIRSL
                     SAAGLEITLIRDVTPIPHNGCRPPKRRRV"
     gene            1911..2540
                     /gene="rpsD"
                     /locus_tag="PKM94_08335"
     CDS             1911..2540
                     /gene="rpsD"
                     /locus_tag="PKM94_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOD66686.1"
                     /translation="MARYRDSKCKLCRREGEKLFLKGARCLTDKCAIERRPFPPGQHG
                     QSRRRKISPYGLQLREKQKIRRLYGLLETQFHNYFEKAAQKKGVTGELLLQSLETRLD
                     NLVYRLGFAPSRNAARQLVRHRHVLVDGGIVDIPAYSVRPGQVIRIKEKSRNLDLIHT
                     SLKEVGRAGDLPWLRLNKASLEGELLEVPKRSDIPLTVNESLVVELYSK"
     gene            2563..3558
                     /locus_tag="PKM94_08340"
     CDS             2563..3558
                     /locus_tag="PKM94_08340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOD66687.1"
                     /translation="MKWKPLTMPKEVVNDQSSANENYARFIVEPLERGYGTTLGNSLR
                     RVLLSSIQGAAVVSMRIRNVLHEFSSVEGVYEDVSDIVLNVKQIRVRMHADEMRTLTL
                     RRHGKGKLTAGMFEQNPEVEILNPDQHICEITGDIDFEMEIDIDSGRSYNVAEQNKRA
                     DAPVGTIFVDSLFSPVIKVAYQVENTRVGQKTDFDRLIMEITTDGSITPEDALSYGAK
                     LLKDHLQLFIHLDEEVMVEEEPQEDEETVRVRNLLRTRVDELELSVRSSNCLRAANIQ
                     TIRDLVTKTEPEMLKYRNFGRKSLNEISTLLEEMGLSFGMDVSKYDETQKQNVNA"
     gene            3578..3997
                     /gene="rplQ"
                     /locus_tag="PKM94_08345"
     CDS             3578..3997
                     /gene="rplQ"
                     /locus_tag="PKM94_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOD66688.1"
                     /translation="MGHQDKIAKLGRTKSHREAMLRNMAMSLFEHRVIKTTDAKAKAL
                     RPLVDRLITNAKKGTLASKREVARTVHQKRIFKKLFAEIVPQFEDRASGFTRVVKLGV
                     RRGDGAALSVVELLTERPKEEPVKKAKKGKEKKGAAR"
     gene            4189..4671
                     /locus_tag="PKM94_08350"
     CDS             4189..4671
                     /locus_tag="PKM94_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66689.1"
                     /translation="MNQESPQQKKGMSTGCLVALIIVGVLVILVVVAGIVCYMKRADL
                     VQYGAVTMTEEIKQMVADKPQPGVDTVQVNAAADAFLAEARAQREPDAEGLALFIQSI
                     QHIRNDGALDSLEAYQFIEAISRAYPNVNLGQAPAAESAPADTMGGEAAPADTAGAAE
                     "
     gene            4779..5447
                     /gene="plsY"
                     /locus_tag="PKM94_08355"
     CDS             4779..5447
                     /gene="plsY"
                     /locus_tag="PKM94_08355"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOD66690.1"
                     /translation="MMWIGPIAVAYLLGAIPFGLLVPRLFGVADIRRHGSGNIGATNV
                     TRVLGFRRAVWVYGLDIAKGAAAVLLGRWYAAAATIPFAGADTYVVLCGLAAVLGHLF
                     PVYLRFRGGKGVNTALGVMLALLPVPALLAFGVFAAVFAIGRYVSLASLVAAVAFPVF
                     ILIEKYAFHRPVASVYLWVSAAVVLLIVYAHRGNIGRLRAGTEHRFGGRRQPADSQGA
                     GRHV"
     gene            5440..6443
                     /locus_tag="PKM94_08360"
                     /pseudo
     CDS             5440..6443
                     /locus_tag="PKM94_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291868.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
     gene            6469..6819
                     /locus_tag="PKM94_08365"
     CDS             6469..6819
                     /locus_tag="PKM94_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOD66691.1"
                     /translation="MKKTNANEKLYYSISEVARITGLEPYVLRYWEKEFPTLCPRKTR
                     GGNRIYTQKDIEVVNRIKHLRTEEKLTIAGARTKLTMRRPSESKETMVASARTKTLIG
                     QIRKDIEDILKDFS"
     gene            6887..6964
                     /locus_tag="PKM94_08370"
     tRNA            6887..6964
                     /locus_tag="PKM94_08370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6922..6924,aa:Pro,seq:ggg)
     gene            complement(7058..8482)
                     /locus_tag="PKM94_08375"
     CDS             complement(7058..8482)
                     /locus_tag="PKM94_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD66692.1"
                     /translation="MKKARPTEARGGRAAAPSAPLESLAGSLRGTLIRPGDSRYDAAR
                     SVWNSMIDRTPAAIVQAADSADVRHTVRFAREHRLPLSIRGAGHNIAGNAVCDDGLTL
                     DLSGLKAVQVEPAARRAWVGAGATLGDFDRAAQDFGLATPLGINSTTGIAGLTLGGGF
                     GWLTRKYGTAADNLRGAEVVLADGSLVRASDTEHPDLFWAIRGGGGNFGVVTRFEFDL
                     HPVGPNVYSGLVVYPHAEAKAVLRQYRDYVAELTDESACWLVLRPAPPVPFVPAEKHG
                     QNVLVVIFFHAGDPEAGRRAAEPLRHLGRPVGEALGVQPYTGWQSAFDPLMTAGARNY
                     WKTHNFTALPDALLDVLVRLADRVPSPECEVFLGQLGGAAARIPRETIAYPHRDADFV
                     LNVHARWRAAEDDERCIAWARELFRATEPFATGGAYVNFVTADESDRIKGIFGANYDR
                     LRRVKRAYDPDNLFRMNHNIAPGR"
     gene            8750..9430
                     /locus_tag="PKM94_08380"
     CDS             8750..9430
                     /locus_tag="PKM94_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD66693.1"
                     /translation="MDENRGEATLVLIPACNAGRYLDDLIPRCRRFVADEHLLVVNDG
                     STDDTLERLKRHRVNYISYPDNRGKGAALRAGYRYALARGYRSVVTLDADLQHLPEEL
                     PRFFAQDNGRRIVLGTRHMDPKVMPWDRRLTNNLTSLIISVFSAQRVRDSQSGYRLIP
                     TAVLRAIPLVAAGYDFESELLFKAGALGCEIVETPISTVYEGSPSFINPVADTGRFIR
                     QIWRRIWV"
     gene            9484..9948
                     /locus_tag="PKM94_08385"
     CDS             9484..9948
                     /locus_tag="PKM94_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD66694.1"
                     /translation="MKLAEIFSNYFTVRGELRKAVEGLTQAQFDWAPPRHTASIGRLL
                     AHIADCEFFWIAHVAAGAPGRPDFTRFRGARDRAEILGLLDEYECVVTGFLEREDLAD
                     WDQVFYTVTDSSGATERLSKRWLVWHVVEHQARHRGQIFMLMRMQGLEVPNV"
     gene            9941..10702
                     /gene="surE"
                     /locus_tag="PKM94_08390"
     CDS             9941..10702
                     /gene="surE"
                     /locus_tag="PKM94_08390"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOD66695.1"
                     /translation="MSERLRILLTNDDGYQADGIATLYRVLARAHEVYLVAPNAEQSA
                     ASHSLTLNRPLRVQKIDERRYTTDGTPTDCVMLGVHLVFKDGKPDMIISGINHGANMG
                     DDVTYSGTVAAAIEGSIMRVPSVAVSMAHYEPGTPMVRAANFVARLVRIVGQMNLPPD
                     VFLNVNLPLDTGRPYGAYEFTRQGFRQYKDVIIRKTDPRGKPYYWIGGRPKWRTEAGT
                     DFEAVSRNVVSITPLRLNFTDDRVLAELRTRQFSM"
     gene            10966..14538
                     /locus_tag="PKM94_08395"
     CDS             10966..14538
                     /locus_tag="PKM94_08395"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66696.1"
                     /translation="MTARGRLGCLFLVASGVWGSVSAQEFTPVRLTAHAGTSANPRIE
                     MDATCLYVVWEDDRSGSSQIWWGKFDLAGAPVAGPVQVTSSGTPSQPAVGVDALGNSY
                     VVWTTDAAYFGSIYGAKLDPEGNVIVPAQQVSEGQVTNPDVAVTPDGVCIVVGDKRGT
                     TDHNVHIYRRTSDLGPVCVAKKPGTAGIGDGRVPAVEADASGIAHVLFRDVDIFNYYI
                     KGHNTDAGCGPSGSTTVLTGDYARPAIAWSGVQEWRTVESGNNIYTIAGGLHRINDQA
                     GTASYARLANTSPNGLAVFQDSRSGDFDIYCTAFNGATGFPDLRVTADPAAQQYPDVA
                     VLPSLPGEFWVVWQDYRDGNWEIYLTRSLGDEPHLALEPLHFGFAAVPGGPLPAGQEL
                     TITISGGGTPAWEADSDQPWLNVTPASGTGSGSTVNLSVNTTALPAGTYEANVAVACA
                     EADNSPQTATVTYVVWGGSELDDKQALIDFFLAEPFYEDAEQAAQVFLDEVEAEVAAG
                     TATSKDLEAVLRLLLLEEGAKAAYGSPEEPGADSLAVLTAKCTLKTATSLLLKLAVDK
                     LERVLRPLKHVWGFNWCYDKVDQATRWLSAVLHRFNSHLVTSYFNQMTPLLMGQYGLA
                     QAEAQSLALQTGWQMVHAIEAKTGKVVLEQVSEPLLQGLLADLNRWLYETGIPLYVPP
                     SSPPDALYIGGTKHSLEAGVTHASGGDFQEGGAEEIIASVRGVEIPEIEGGNSVAVQT
                     VNGAITAAVWAEIAELILLAATVIGLAVALAVGIALCGSTGGIGCVISALSGVGLWGA
                     LLKIANLLSWGKIALYGLGMGVGAYQLHVHLPAQLREVKQTAFDVVPKVLADSGLSAG
                     PQRFRIPGQWSDSLETAASGAADRLEEIRRLIAAGDWDSARASMAALDSAMDVLATAS
                     AITNATLGCAVIGNQSDTLPLLDSLYGYAMAHGAANDLTAALCQLALGFASVAEPAGA
                     DCDSLLMLVDETTARLRQLGPAFDSAFTVLADMGLTAPPVVVVTEYELAEDGLGKHLT
                     ATVRNVSETAVHEVRAGLAAEDSSSVQATAETDTVIAALAAGDTGVFRWRIEVPAAET
                     LFQMDLLIQPGTLPGDFLGDHRIVSVHFPAATYEYRCGDVTADGQITVADVTYLVAYL
                     FRGDPAPVLPGAANVNGESGINVADVTFLIAYLFRGGPAPEACP"
     gene            14666..14739
                     /locus_tag="PKM94_08400"
     tRNA            14666..14739
                     /locus_tag="PKM94_08400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14700..14702,aa:Pro,seq:cgg)
     gene            15156..15650
                     /locus_tag="PKM94_08405"
     CDS             15156..15650
                     /locus_tag="PKM94_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOD66697.1"
                     /translation="MPEGQRPVVIAIVGAGKCSKKLRDMAAAAGRYVAAHGGVVVCGG
                     LGGVMEGAARGAKEAGGATIGILPGMDKNDANDHIDYVIPTGFGEARNILVVRAADAV
                     IAFPGKYGTLSEMAFTMQMGKPLISVGAWKLGEGIPLVEDPEEAAKMAMELATGAPQG
                     AETE"
     gene            15675..15953
                     /locus_tag="PKM94_08410"
     CDS             15675..15953
                     /locus_tag="PKM94_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOD66698.1"
                     /translation="MKLAGAIVTVKGEVQGVGFRWWALKRAHLRGVTGWVRNNRDGSV
                     TALAEGERGALDAFIDDLREGPSLAVVRAVDIDWQEYTGTYAGFDITH"
     gene            15928..16491
                     /locus_tag="PKM94_08415"
     CDS             15928..16491
                     /locus_tag="PKM94_08415"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOD66699.1"
                     /translation="MRDSTSHTSLGAGGEELKRYIRSVPDFPRPGINFYDITTLLQNP
                     AGFRRAVDQLEGYVRGVQAERILAIESRGFFFGAALADRLGLSIIPARKPGKLPYTTV
                     SEEYELEYGTDRLEVHADAVLPGDRVVVVDDLIAIGGTLLAACRLVERLGGVVAGVAA
                     VIDLRFLPWREKLSGYDVRYLVSYDSE"
     gene            16559..16635
                     /locus_tag="PKM94_08420"
     tRNA            16559..16635
                     /locus_tag="PKM94_08420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16593..16595,aa:Arg,seq:cct)
     gene            16835..18370
                     /locus_tag="PKM94_08425"
     CDS             16835..18370
                     /locus_tag="PKM94_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOD66700.1"
                     /translation="MKSLTVKGTVVLLLIGTTVFAQGGVGLEYGNRLEFDVWDFSRPA
                     LEGYQIDFSGGGARALGMGRAFLGLSDDVTAISWNPAGLYTLSQPVISATYGSTLPRG
                     YTDTDPQFVLDPSQTRFDHNGWISGITALDFAAPLRIKGHPFVGSFTYSTGFDEYQGY
                     QFSFDTTFRDTFTTPDTIFVDTVVHYTVNRESEMDGALKAVSFGFGTRIYREWSVGAA
                     LNVYSGTAVRHAASYESSQDRIDYSFQEYSQVYATTVIDSNRFSGYNFTLGVRYNASP
                     LAAGLIIRTPFDLEVETDRSIYEIAKKNERVAAQETDTFYFDDMVTKYEMPWMIGAGV
                     AYKANERTVLTADVEYRRFSTQGVLLRDSVKIDPGGENEEFFTTVAADTLWKDVLQVA
                     VGAEYLWDQTWGTIPLRFGLAYVPTIVPSYERDESDTANAVLGLDSASVTGRLVAKQE
                     RTNGVRLSAGAGIWWKQVHLDFAYSYLTRDWDTGVPGTVRTGAFHQRIRNHHVSATFT
                     GYF"
     gene            18499..19188
                     /locus_tag="PKM94_08430"
     CDS             18499..19188
                     /locus_tag="PKM94_08430"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66701.1"
                     /translation="MQSKVKLSKRQIKEDKFTAFVLTSKQRVAENWQFLVIGGVVLVL
                     AVVAVVYFASSRTSREAEGSRLFAQGMAEYRNGNRQVALTTLQEVVDEHGGHETADNA
                     TYLLGQIQFELRNFAEAIRWWEQYLAKYRDNRTDRAAALAGIAAANEEQGNNAAAAQK
                     YREAAAEYPEGPLAWDYHTGAMRNYLAAGQVDKAREELDSLRDKYAGTEIYNRAARLF
                     AAQAVPAKTAP"
     gene            19218..>21150
                     /locus_tag="PKM94_08435"
     CDS             19218..>21150
                     /locus_tag="PKM94_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOD66702.1"
                     /translation="MPIKVALLWHMHQPNYQEPQSRRLVLPWVRLHAVKDYLDMPLLA
                     AEQPNIRVTFNLVPSLLDQFQLYVDGGVDRHLELSRVPSDQLDADLRREILTTFFAGH
                     PATMIEPYARYAELYRKYRSNLGESVLPALFTSEEFRDLQVWSNLAWVDPLFRDEEPI
                     RSLLARGRYFTEGHKHALLDWQVQFIARIVPTYQRLFREGRIEVSFTPYYHPILPLLC
                     DTDVAREAIPGTNLPRRFRHPEDAEKQIVMAQEKYRELFGREMAGMWPSEGSVSEEVA
                     ALCAKNGVSWIATDEEILTASLKKSGLDRADNPPHAVYQHSTGVRMLFRDHALSDRVG
                     FVYSGWEAERAVQDFVAQIKEIGRRTLAAGVEPVVPVILDGENAWEYYPADGRDFLRL
                     LYHQLATDPDIRTVTISEATEGVAARPLPKLFAGSWINHAFRIWIGHEEDNVAWDLLS
                     DARDALVKYQEERPAADPAALAAAWRQIYIAEGSDWCWWFGDEHRGFDNKTFDHIFRR
                     HLVAVYRLLGLDVPDRLWEPIYQGETKSRTVLPDALLTPAIDGRVTHFYEWAGAGMYD
                     CAEAGGSMHRVDRLVSAIYFAYDHERLYIRVDFVNRKALLSIVEPAVEVVFQAPQPVR
                     LNAPVAHGGRRVDAEKGYEV"
BASE COUNT         3852 a         6344 c         7403 g         3551 t
ORIGIN      
        1 accgtcatcc ggccgggaat gacgaccggg cagctcgacc gcctcatcga ggacttcatc
       61 cgggccgagg gagcgatccc ggccttcaag gggtaccagg gattcccggc ctcggcgtgc
      121 atttcgatcg acgacgaggt cgtgcacgga attccgggga aacgggtgat cgaggagggg
      181 gtgatcgtgt cggtcgatat cggcgcgatc gtggatgact actacgggga ctcggcgcgg
      241 accttcggca tcggcgaggt gtcggaggag aagcggcggc tcatggagtg gacgctgaaa
      301 gcgctccacg ccggtattga caaggcgcga aaagggaata gattaggcgc ggtatcggcg
      361 gccatccagc aggtggcgga gtcgcaggga tacggtgtgg tgcgccaact ggtcggccac
      421 ggcatcggcc gcgccatgca tgaggagccg caggtgccca atttcggctc gcccgctgac
      481 ggcccggtgc tcgaggcggg aatggtcatc gcgatcgaac cgatgatcaa catggggacg
      541 tacgaggtca ggacgctgcc ggacgggtgg acggtggtga cggcggacgg acgtccgtcg
      601 gcccatttcg aacacaccgt cgcggtgggc ccggacggcc cgcggatcct cacggcggcc
      661 taaaggagcg tatggcgaaa gaagagacga taacggtgga aggccgggtg atcgagccgc
      721 tgccgaacgc cactttcaaa gtggagttgg acaacggaca cgtggtgctg gcgcacattt
      781 cggggaagat gcggatgcat ttcatcaaga tcctccccgg cgacaaggtg acgctggaac
      841 tgtcgcccta cgatctcacg cgcggccgga tcacgtatcg ctataaatga gtaaccccag
      901 acgggatcgg tggtcggtat gaaagtcaag tcgtcggtca agaagcgctg cgagtactgc
      961 aagatcattc ggcgccatgg cgtgatccgg gtcatctgca agcgcgaccc cagccacaag
     1021 cagcgccagg gttgagaatt gtgtgtgtgc gatagagaag tgaggaaaaa ccattggcac
     1081 gtatagccgg agttgatctt cccaaggaca agcggatcga ggtcgggctg acctacatct
     1141 acgggatcgg cccgcaccgg gcgaggctga ttctcgccaa gacgggagtg aatcccgaca
     1201 cccgggtcaa ccagctgacc gacgaggacg tgacgaagct gcggcaggtc atcgagaacg
     1261 agtacagcgt cgaggggtcg ctgcgcggcg agatccagat gaacatcaag cggctgatcg
     1321 atatcggcag ctaccgcggc ctgcgccacc ggcgcggcct ccccgtgcgg ggacagcgga
     1381 ccaagacgaa cgcccggacg cgcaagggtc cgcggcgctc gatcggcggg ctcaagaaaa
     1441 aacctgttgg caagaagtaa gcgacccgga ggagaacagt cgtggcggaa ccgaaaaaga
     1501 agggacgacc gagaaagaag tcccgcaagg ttgatacgaa cggcgtggcc cacatcaagg
     1561 ccactttcaa caatacgatt atcacgctga ccgatgcgca gggtcgggcg atctcgtggg
     1621 ggacggccgg caaggtgggg ttcaaggggt cgaagaagtc gacgccgttt gcggcccagc
     1681 aggccgcggc cagcgctgtg cgcgaggcca tggaccaggg gctgcggcgg gtcgaggtct
     1741 gggtgaaggg gccgggatcg ggccgcgaag ccgctatccg ctcgctctcg gcggccggtc
     1801 tcgagatcac cctcatccgc gatgtgaccc cgattcccca taacggctgc cgcccgccca
     1861 agcggcgccg cgtgtgagca aatgaaacgt ggtcggaaat aggagagcca atggcacggt
     1921 atcgtgactc gaaatgcaaa ctgtgtcgcc gggaaggtga gaagctgttc ctcaaggggg
     1981 cgcgctgtct gacggacaag tgcgccatcg agcggcggcc ttttccgccg gggcagcacg
     2041 gccagagccg ccgccggaag atttcgccgt acggcctcca gctgcgcgaa aaacagaaga
     2101 ttcggcggct gtacggactg ctcgaaaccc agttccacaa ctacttcgag aaggcggccc
     2161 agaagaaggg cgtcaccggc gaactgcttc tgcagtcgct cgagacgcgc ctggacaacc
     2221 tcgtctaccg gctcggcttc gcaccgtcgc gcaacgccgc ccgccagctc gtccgccatc
     2281 ggcacgtcct ggtcgacggc ggcattgtcg acattcccgc ctacagcgtc cgtcccggcc
     2341 aggtcatccg gattaaggaa aagtcgcgga atctcgactt gattcatacc tccctgaagg
     2401 aagtgggccg ggccggcgac ctgccctggc tgcggctgaa caaggccagt ctcgaaggcg
     2461 aactgctcga agtgccgaag cggtcggaca ttccgctgac ggtgaacgag tcattggtcg
     2521 tagagctgta ctccaagtaa tgttgggagg gccgagcaag ctatgaagtg gaagccattg
     2581 accatgccga aagaggtcgt taacgaccag tcctcggcca acgagaacta cgcacggttt
     2641 atcgtcgagc cgctggagcg cgggtacggc acgacgctgg ggaacagtct ccggcgggtg
     2701 ctgctctcct cgattcaggg cgcggcggtc gtgtcgatgc ggatacggaa cgtcctgcac
     2761 gagttctcca gcgttgaggg cgtctatgag gatgtgagcg acatcgtcct caacgtcaag
     2821 cagatccggg tgcggatgca tgccgatgag atgcgcacgc tcacgcttcg ccgccacggg
     2881 aaggggaaac tcacggccgg gatgttcgag cagaacccgg aagtcgagat cctcaacccc
     2941 gaccagcaca tttgcgaaat caccggcgat atcgacttcg aaatggagat cgacatcgac
     3001 tccggccgca gctacaacgt cgccgagcag aataagcggg ccgacgcccc ggttgggacg
     3061 atcttcgtcg attccctctt ctcgccggtg atcaaggtgg cctaccaggt cgagaacacc
     3121 cgggtcgggc agaagaccga tttcgaccgc ctgatcatgg agatcacgac cgacggttcg
     3181 atcacgcccg aggatgcgct gtcgtatggg gccaagctgc tgaaggacca cctccagctg
     3241 ttcatccacc tcgacgaaga ggtgatggtg gaggaggagc cgcaggagga cgaggagacg
     3301 gtgcgggtgc gcaatctgct gcggacgcgg gtggacgagc tggagctgtc ggtccggtcg
     3361 tcgaactgcc tgcgggcggc caacatccag acgatccggg acctggtcac caagaccgag
     3421 ccggagatgc tgaagtaccg gaattttggg cggaagtcgc tgaacgaaat ctccacgctc
     3481 ctggaggaga tggggttgtc gttcgggatg gacgtgtcga agtacgacga gacgcagaaa
     3541 cagaacgtga atgcctaagc tgaagtgaag atttgcgatg ggacatcagg ataaaatagc
     3601 aaaactgggg cgaaccaaat cgcaccgcga ggccatgctc cgcaatatgg cgatgtcgct
     3661 ctttgagcac cgggtgatca agaccaccga tgccaaggcg aaggcgctgc ggccgctggt
     3721 cgaccggctg atcaccaatg ccaagaaggg gacgctggcg tcgaagcgtg aggtggctcg
     3781 cacggtccat cagaagcgaa tcttcaagaa gctcttcgct gagatcgtgc cgcagttcga
     3841 agaccgcgcg tcagggttca cccgggtggt caaactgggt gtgcggcgcg gcgacggggc
     3901 ggccctgtcg gtggtcgagc tgctcaccga acggcccaaa gaagagccgg tgaagaaggc
     3961 gaagaagggc aaggagaaaa agggggcggc tcggtagacc ggcaactcag cccccgcggc
     4021 catccgctgt gatactgaaa gcccgcccct ccggcgggct ttgtgttggt cggccggggc
     4081 gccggccgaa gtgcgcaacg tttgcattaa agacttgcca gaacccgctg ccggtgctac
     4141 ccttatccca ggtaacccaa agtcatccgt aaactcaaac gaggggttat gaatcaggaa
     4201 tcgccgcagc agaagaaagg gatgtcgacc ggctgtctcg tcgcgctcat catcgtcggc
     4261 gtgctggtca tcctggtggt ggtcgccggg atcgtctgct atatgaagcg agccgatctg
     4321 gtgcagtacg gagcggtcac gatgaccgaa gagatcaagc aaatggtagc cgacaaaccg
     4381 cagccgggcg tcgatacggt ccaggtgaac gccgcagcgg acgcgtttct cgccgaggcg
     4441 cgggcgcagc gggagccgga tgccgaagga ctggcgttgt tcattcagtc gatccagcat
     4501 atccgcaacg acggggcgct cgattcgctg gaggcctacc agttcatcga ggcgatttca
     4561 cgcgcctacc ccaacgtgaa tctggggcag gccccggccg cggagagcgc gccggccgat
     4621 accatgggcg gcgaggcggc gccggcggat accgccgggg cggccgagta ggcaccggga
     4681 gtgaagcggc ggccggatga gccctttcgg tcgcccagcg gccgggcggc cgcggcgccg
     4741 ggggcagcgg gcagaagagg cggaaggacg accgcatcat gatgtggatc ggaccgatag
     4801 ctgtggccta cctgttggga gccatcccgt tcgggctgct cgtcccgcgc ctgttcggag
     4861 ttgctgatat ccgccggcac gggtcgggaa atatcggcgc caccaacgtc acgcgcgtgc
     4921 tcggctttcg ccgggctgtc tgggtgtacg gactggacat cgccaaaggg gcggccgcgg
     4981 tgctgttggg acgatggtat gccgcggcgg cgacgatccc attcgcggga gccgacacct
     5041 acgtcgtcct ctgcggtctg gctgcggtgc tcggacacct cttcccggtc tacctccggt
     5101 tccggggggg gaagggggtc aacacggcgc tcggggtgat gctggctctc ctgccggttc
     5161 cggcgctcct ggcgttcggc gtattcgcgg ccgtgttcgc gatcgggcgc tacgtttcgc
     5221 tcgcctcgct ggtggcggca gtggcgtttc cggtgttcat tctcatcgaa aagtacgctt
     5281 tccaccggcc ggtggcctcg gtctatctct gggtatcggc ggccgtcgtg ctgctcatcg
     5341 tgtacgccca ccggggcaac atcgggcggc tgcgggccgg gacggaacac cggttcggcg
     5401 gccgccggca gccggccgat tcgcaggggg cgggccgcca tgtctgagcg ggtcgccatc
     5461 ctcggggcgg ggtcctgggg gctggccgtg gctcgtctgc tgcacgccaa cgggcaccgc
     5521 gtccggctgt gggagttcga ccccgccgag catgccctcc tgctccgcca ccggactcat
     5581 ccgaaaaagc tgcccgggtg ccggctcgat gagcagattg gcatcaccaa cgatctcggc
     5641 gaggcggtcg ccggagcgga cctgatcgtg ctggcgatcc catcccagcg gatgcggtcg
     5701 gcgctcgacc cgctggccgg ccgggttggc cccgacgtcg gcatcgtcaa cctggccaaa
     5761 gggatcgaga tccgcacgct caagcgcatg tcggaggtgg tggccgaggt cctcgatcga
     5821 ccgctcgggc gcatcgccac cctctccggg ccgtcgcacg ccgaagaagt ggccagggac
     5881 atgccgacga ccgtggtggt cggcggtgtg aacgaagagt tctgcgcgga ggtccaggag
     5941 accttctcag gacagacctt ccgcgtgtac tgctgcgacg acctgaccgg cgtggagttg
     6001 gggggcgcgc tgaagaacat catcgcaatc gccgccggga ttgcgggggg actcgggctg
     6061 gggacaacac cctgggggcg ctcatcacgc gcggcctggc cgagatcacc cgcctcggcg
     6121 tggcgatggg ggccaacccg ctgacgttct cggggctgtc gggggtggga gacctggtga
     6181 ccacctgcgt gtcgcggcac agccgcaacc gctatgtggg cgaacacatc ggcaggggcg
     6241 aaacgctggc gcgggtgctg gcggggatga cgatggtggc cgagggagtg gagacgaccc
     6301 gttcggggca tgccctggcg aaccggcacg gcgtggagat gccgatcacg gcgcaggtac
     6361 acgccgtctt gttcgataac aagccgccgg ccgaggcggt ggcggagctc atgggcagag
     6421 aactgaaacc ggaaatctgg taaggaaccg catcgcgagg agcggtccat gaagaagacc
     6481 aacgccaacg agaagctgta ctactcgatc agcgaggtgg cccgcatcac ggggctcgaa
     6541 ccttacgtgc tgcgatactg ggagaaggag tttccgacgc tctgtccgcg caagacgcgc
     6601 ggggggaacc ggatctacac gcagaaggac atcgaggtgg tcaaccgcat caaacacctg
     6661 cgcaccgagg agaaactgac gatcgccggg gcgcggacca agctgacgat gcggcggccg
     6721 agcgagagca aagagaccat ggtggcctcg gcgcgaacga agacgctgat aggccagatt
     6781 cgtaaggata tcgaggacat cctcaaagat ttctcttgaa ctcggcggcg gttttgatag
     6841 attgggacgg gcgtaaccgg cgcgggacag tctagatccg atggatcgga gcgtagcgca
     6901 gtttggttag cgcactcgct tggggtgcga gaggtcggcc gttcgaatcg gctcgctccg
     6961 accattttgc cggccgggcg accggcggaa gctcccacga caacgaaaag gccggacgac
     7021 gcgggcgcat cgcccggccc tgatgtcggc cgggcgctta ccgccccggc gcgatgttgt
     7081 ggttcatccg gaacagattg tcggggtcat aggcgcgctt gactcgccgg agacggtcgt
     7141 agttggcgcc gaagattccc ttaatgcggt cggactcgtc ggcggtcacg aaattcacat
     7201 aggcgcctcc ggtggcgaaa ggctcggtcg cccggaacaa ctcgcgcgcc caggcgatgc
     7261 agcgctcgtc atcctcggcc gcccgccagc gggcgtggac gttgaggacg aagtcggcgt
     7321 cgcggtgcgg gtaggcgatg gtctcgcgcg ggatgcgggc cgccgccccg ccgagctggc
     7381 cgaggaagac ctcgcactcg ggactcggca cgcggtcggc caagcgcaca agcacgtcga
     7441 gcagggcgtc gggcagggca gtgaaattat gcgtcttcca gtaattccgg gcgcccgccg
     7501 tcatgagcgg gtcgaaggcc gactgccagc ccgtgtacgg ctggacgccg agcgcttcgc
     7561 ccaccggccg cccgagatgg cgcagcggct cggcggcccg ccggcccgcc tcgggatcgc
     7621 cggcgtggaa aaagatcacg acgagcacat tttgcccgtg cttttccgcc gggacgaacg
     7681 gaaccggcgg ggcggggcgc aagaccagcc agcacgcgga ttcgtcggtg agttccgcga
     7741 catagtcgcg atactgccgg agcaccgcct tcgcctcggc gtgcgggtag accaccaggc
     7801 cgctgtacac gttggggccg accgggtgca ggtcgaattc aaaccgggtg acgacgccga
     7861 aattgccccc gccgccccgg atcgcccaga acagatcggg atgctcggtg tcgctggccc
     7921 gcaccagcga gccgtcggcg agcacgacct cggcgccgcg gaggttgtct gcggctgtcc
     7981 cgtactttcg cgtgagccat ccgaagcccc cgccgagcgt gaggccggcg atgccggtcg
     8041 tggagttgat gccgagggga gtggccagtc cgaaatcctg ggccgcccgg tcgaagtcgc
     8101 cgagcgtggc gccggccccg acccaggcgc gacgggcggc cggctccacc tgcaccgctt
     8161 tcagcccgga caggtccagg gtgagaccgt cgtcgcagac cgcgttcccg gcgatgttgt
     8221 gcccggcgcc gcgaatggac agaggcaagc ggtgctcccg ggcgaagcgg accgtgtgcc
     8281 ggacatcggc ggaatcggcg gcctgcacga tggcggccgg tgtccggtcg atcatgctgt
     8341 tccagaccga gcgggccgcg tcgtagcggc tgtctccggg gcggatcagc gtgccccgca
     8401 gcgaaccggc gagcgactcg aggggggccg aaggggcggc ggctcggccg ccgcgcgcct
     8461 cggtgggacg ggcttttttc atcccgcgtc tccttcctgt gcggcagacc gcccggtcgc
     8521 ccgccgccga catgatttcc tgagcttggc cgcagccgat cggccgcggc cgcatgttgg
     8581 ccgttgtctg tccggtgata cgcggatccc cggccgtctg attgtggtcc aaatgagtct
     8641 ttaaaccaac gtccggccgg ctcgcagccc ggggctttcg cccgacctgc tctccgatcg
     8701 tgaacttttt gtgtcgcggg cgcccggccc ttccgtataa tgacctgcga tggacgagaa
     8761 tcggggcgag gcgacgctgg tgctcatccc ggcgtgcaac gccgggcggt atctcgatga
     8821 cttgatcccc cggtgccgcc ggttcgtggc cgacgaacac ctgctcgtgg tcaacgacgg
     8881 atcgaccgac gatacgctcg agaggctcaa acggcaccgg gtcaactaca tctcgtatcc
     8941 cgacaaccgg ggaaaagggg cggccctgcg ggcgggttac cggtatgcgc tcgcgcgcgg
     9001 gtaccgctcg gtcgtgacgc tcgatgccga cctgcagcac ctgccggagg agttgccccg
     9061 tttcttcgcc caggacaacg ggcggaggat cgtcctcggg acgcggcaca tggatcccaa
     9121 agtgatgccc tgggaccggc gcctcaccaa caatctgact tcgctgatca tctccgtgtt
     9181 ctccgcgcag cgcgtccgtg acagccagtc gggctaccgc ttgatcccga ccgcggtgct
     9241 gcgggcgatc ccgctggtcg cggcggggta cgatttcgag tcggagttgc tgttcaaggc
     9301 gggagcgctc ggctgcgaga ttgtcgaaac cccgatttcc accgtctacg aggggtcgcc
     9361 gtcgttcatc aacccggtcg cggacaccgg gcggttcatt cgccagatct ggcggcggat
     9421 ctgggtctga ccggcgccaa ttcgggcttg cgaagggcgg cgatcggcgc taccatacag
     9481 ggcatgaagc tagctgagat tttctccaac tatttcacgg tgcgcggcga attgcggaag
     9541 gcggtggagg gtctgacgca ggcgcagttc gattgggcgc ccccgcgcca cacggcatcg
     9601 atcggacgac tcctggccca catcgccgac tgcgaattct tctggatcgc ccacgtggcc
     9661 gccggcgcgc ccggacggcc ggatttcacg cgctttcgcg gagcgcgcga ccgggccgag
     9721 atcctcggcc tcctcgacga gtacgagtgc gttgtcaccg ggttcctcga acgggaagac
     9781 ctggccgact gggaccaggt gttctacacc gtgaccgaca gcagcggggc gaccgagcgc
     9841 ctctcgaagc ggtggctcgt gtggcacgtg gtggaacacc aggcgcgcca ccgcggccag
     9901 atcttcatgc tcatgcgcat gcaggggctt gaggtgccga atgtctgaac ggctccggat
     9961 tttgttgacc aacgacgacg gctaccaggc ggatggaatc gcgaccctct accgggtgct
    10021 cgcgcgggcg cacgaggtct acctggtggc cccgaacgcg gaacagtcgg ccgcctcgca
    10081 ttcgctcacc ctcaaccggc cgctgcgggt gcagaagatc gacgagcggc gctacacgac
    10141 cgacgggacc ccgaccgact gcgtcatgct cggcgtgcac ctggtgttca aggacggcaa
    10201 gccggacatg atcatctcgg ggatcaacca cggcgccaac atgggggatg acgtgaccta
    10261 ttcggggacg gtggcggcgg cgatcgaggg ctcgatcatg cgggtgccgt cggtggccgt
    10321 gtcgatggcc cactacgagc cggggacgcc catggtgcgg gcggccaact tcgtcgcccg
    10381 gctcgtgcgc atcgtcgggc agatgaacct cccgcccgac gtcttcctca acgtcaacct
    10441 gccgctcgac acggggcggc cctacggcgc ctacgaattc acccggcagg ggttccgcca
    10501 gtacaaggat gtgatcatcc ggaaaaccga cccgcgcggc aaaccctact actggatcgg
    10561 ggggcggccg aagtggcgga ccgaggcggg gaccgatttc gaggcggtga gccggaacgt
    10621 ggtgtcgatc acgccgctgc ggctcaattt cacggacgac agggtgctgg ccgagctgcg
    10681 cacgcggcaa ttctcgatgt agggggaaac gaacactgcc gcacgggggt gtggccgtgc
    10741 gatatagatc gtcggtttcg agagctggaa gacggtgccg tgcgccgtgc cacatgccca
    10801 gaaggggtct taccatgggg cagagcgtgc gtcccgttcc ggcgcgccgg gctgtcgtat
    10861 caggttgcct ctgaacagca cgacgtatct ccgatatgat gtggctgaca tctgcgcgag
    10921 tgacgtgtca cacgtccttc gcaagtttga tccggaggtg attgtatgac cgcacgtgga
    10981 cggttgggtt gcctgttcct ggtggcgagc ggagtgtggg ggagtgtgtc ggctcaggag
    11041 ttcacgcccg tccggctcac tgctcacgcg gggacgtcgg ccaatccgcg catcgagatg
    11101 gatgccacct gcctctatgt tgtctgggaa gacgaccgtt ccgggtccag tcaaatctgg
    11161 tgggggaaat tcgacctcgc cggcgcgccc gtcgccggcc ccgtacaagt gacctcgagc
    11221 gggactccct cccagccggc ggtgggtgtc gacgcactgg gaaacagcta cgtcgtctgg
    11281 accaccgacg ccgcctattt cggcagcatc tatggtgcga agctcgatcc ggaaggaaat
    11341 gtaatcgtcc cggcccagca ggtatcggag ggtcaggtca ccaatccgga tgtggcggtc
    11401 acaccggacg gcgtctgtat agtggttggc gacaagaggg ggaccacgga tcacaacgtt
    11461 cacatttatc ggcggacgag cgacctcggt ccggtttgcg tggcgaagaa accgggaact
    11521 gccgggatcg gcgacggtcg ggtgccggcc gttgaagcag acgcgtcggg aattgcccac
    11581 gtgctgttcc gggatgtcga cattttcaac tactacataa agggccacaa caccgatgcc
    11641 ggttgcggtc cctccggctc gaccaccgtt ttgaccggtg attacgcccg gccggcgatt
    11701 gcatggtccg gggtgcagga atggcgcacc gtggagtccg gtaacaacat ctacacaatc
    11761 gcgggcggcc tgcatcgcat caacgaccag gccggtaccg cttcctacgc ccgtctcgcc
    11821 aacacgtcgc cgaacggcct ggccgtgttc caggattcgc gatcgggcga cttcgatatc
    11881 tactgcacgg ccttcaatgg ggcgaccgga ttccccgatc tccgcgtcac tgccgatccg
    11941 gctgcacagc agtatcccga tgtcgcggtc ttgccctccc tgccgggcga gttctgggtc
    12001 gtctggcagg attaccggga cggcaactgg gagatctatc tgacgcgttc gctgggggat
    12061 gagcctcacc ttgcattgga gccgctgcat ttcggttttg cggcagttcc gggcggaccg
    12121 cttcccgccg gccaggagct caccatcacc atcagcgggg gcgggacacc ggcgtgggag
    12181 gccgattccg accaaccgtg gcttaacgtg acccccgcct ccggtaccgg cagcgggtcg
    12241 actgtgaacc tgagcgtcaa cacgacggct ctgccggccg gcacctatga ggcgaatgtc
    12301 gcggtggctt gcgccgaggc ggacaacagc cctcaaacgg cgactgtcac ctacgttgtc
    12361 tggggaggca gcgagctgga cgacaagcag gcgctcatcg acttcttcct cgcagagccg
    12421 ttctacgagg acgccgagca ggccgcgcag gtgtttctgg acgaggtcga agcggaggtg
    12481 gcggcgggca cagcgacgag caaggatctg gaggccgtcc tgcgccttct gctgctcgaa
    12541 gagggggcga aggcggcgta tgggtcgccg gaggagccgg gagcggattc gctcgccgtg
    12601 ctgactgcaa agtgcacgct caagacagcc acatcgctgt tgctcaaact cgcggtggac
    12661 aaactggagc gggtgttgcg gccgctgaaa catgtctggg ggttcaactg gtgctacgac
    12721 aaagtcgacc aggccacgag atggctgagt gccgtactcc acaggttcaa tagccatctc
    12781 gtcacctctt acttcaacca gatgacaccc ctgctcatgg ggcagtacgg gctggcgcag
    12841 gctgaggccc agtcgctggc actgcagacc ggctggcaga tggttcatgc gattgaagcg
    12901 aagaccggaa aagtcgtctt ggagcaagta tccgagccgc tgttgcaggg tctgttggcg
    12961 gacctcaacc gatggctgta cgaaacgggg atacctctct atgttccccc aagttcgcct
    13021 ccggatgccc tctacatcgg ggggaccaaa cactcgctgg aagcaggtgt gactcatgct
    13081 tcgggcggag actttcagga agggggagcg gaggagatca tcgcgtcggt gcggggcgtc
    13141 gagattccgg agattgaggg cgggaattcg gtggcggtcc agacggtaaa cggagcgatc
    13201 acagcggcgg tctgggctga aatagccgaa cttattcttc tggctgccac cgtgataggt
    13261 ctggcagttg ccctggcggt tggtatcgct ctctgcggaa gcacaggggg gatcggatgc
    13321 gtcatcagcg ccctctccgg agtcggccta tggggggcgt tgctgaagat cgcaaacctg
    13381 ctgtcgtggg gaaagatcgc tctgtatggc ctgggaatgg gtgtgggagc ttaccagctt
    13441 catgtccacc tccccgcaca gctccgcgag gtgaaacaga cagcgtttga tgtcgtgccg
    13501 aaggtgcttg cggacagcgg cttgtccgcg gggccgcagc gtttccggat tccggggcaa
    13561 tggagcgatt cacttgaaac ggccgcgtcc ggcgcggccg accgccttga agaaatccgc
    13621 cggctcattg cggcaggcga ctgggattcg gccagagcaa gtatggcggc cctcgattcc
    13681 gcaatggacg tgctggcgac agcctccgct atcaccaacg ccaccctggg ttgcgcagtg
    13741 atcgggaacc agagcgatac tctgccgctg ctggactcac tgtatggtta cgcgatggcc
    13801 catggcgcgg cgaatgacct gactgcggcg ctgtgccagt tggcgctcgg gtttgcctct
    13861 gtggccgaac cggcaggtgc agactgcgac agtctcctca tgctcgtcga cgagacaact
    13921 gcgcggttgc gccaactcgg tccggccttc gactccgcct tcacggtgct cgccgatatg
    13981 ggtctcacgg cgccgccggt ggtcgtcgtt acagagtatg agttagccga ggatggactc
    14041 ggcaagcatc tgaccgcgac cgtgcgcaat gtgtccgaga cggccgtgca cgaggtgcgg
    14101 gccgggcttg cggcggagga cagctcgtcg gtgcaggcga cggcagaaac ggacaccgtg
    14161 attgccgcgc tggccgccgg cgacaccggg gtttttcgct ggcgcatcga ggtccccgcc
    14221 gccgaaacgc tcttccaaat ggatctgctg attcaaccgg ggacgcttcc cggtgatttt
    14281 ctcggcgatc atcggatcgt gagcgttcat ttcccggccg cgacgtatga gtatcggtgt
    14341 ggagacgtca ctgccgacgg gcagataact gtcgctgacg tcacgtacct cgtagcgtac
    14401 ctcttcaggg gagatcctgc cccggtcttg cccggggcgg ccaatgtgaa cggcgagtcg
    14461 gggatcaatg tggccgacgt caccttcctg attgcctatc tcttccgcgg cggacccgcg
    14521 ccggaggcat gtccgtgacg gcgaatttct tgggtcgcac ggtcggctgc gggcgccggc
    14581 tgagcgtctc ttccgccttc gggggtgcaa aatcctgttg acacgggggt aaaataggct
    14641 tacttgaggg ggtctcggaa acaatcgggg cgtagcgcag tttggtagcg cacttggttc
    14701 gggactaagg agtcgctggt tcaaatccag tcgccccgat ttttctcccc ccaccagcaa
    14761 tggtgggcca ccggtcagcg gcgggtgcac gattccacca tgcggggtgg gccgccgaaa
    14821 gagaattctg ccgggcaggg cggacccctc tcagggtcgt cggccccggt cggcgggcgc
    14881 cgcggcgagt gaggcttcac tgtcaggtta gcaggtaaag gactatcaca agggctcgca
    14941 aagggctcgg tttcggctct gccggccggc atcgccgacg gatgcgtccg gcgccgggaa
    15001 agctcatcaa caacagtcat tcatcgaggg tgtgtcgctg cgagacgagg gatcgggcgt
    15061 gcccggtcgg caaggccccg gcccggcgca ccgcgtgaac gcgatatcgg gaagtagaag
    15121 gggagtcacc tcaggagagc gaaagggaag ccacgatgcc agagggacaa cgaccggtgg
    15181 tgatcgccat tgtcggcgcc gggaagtgct cgaagaaact gcgcgacatg gcggcggcgg
    15241 ccggcaggta tgtcgccgcg cacgggggag tggtggtctg cggaggactt ggcggggtga
    15301 tggagggggc ggcccggggc gccaaggaag ccggcggcgc caccatcgga atcctcccag
    15361 ggatggacaa aaacgacgcc aacgaccata tcgactatgt gatcccgacc gggttcggcg
    15421 aagcccgcaa catcctcgtg gtgcgggcgg ccgatgccgt catcgctttt cccggcaaat
    15481 acggcaccct gtcggagatg gcgttcacca tgcagatggg caagcccctg atctcggtgg
    15541 gggcgtggaa actgggcgaa gggatcccgc tggtcgagga tccggaagaa gcggcgaaaa
    15601 tggccatgga gctggcgacc ggcgcgcccc agggagcgga gaccgagtag gatgggtcgg
    15661 gcggaggcgc ggcagtgaag ctcgccgggg caatcgtgac cgtgaaaggg gaggttcagg
    15721 gggtagggtt ccgctggtgg gcgctcaaac gggcgcacct ccgcggggtg acgggctggg
    15781 tgcggaataa ccgggacggt tcggttaccg ccctggccga gggggagcgg ggcgcgctgg
    15841 acgcatttat cgacgatctt cgggaaggcc cttccctggc ggtcgtccgg gcagttgaca
    15901 tcgactggca ggagtataca ggtacgtatg cgggattcga catcacacac tagtctgggc
    15961 gcgggcggcg aagaactgaa acggtacatc cgcagcgtcc cggatttccc cagaccgggg
    16021 atcaatttct acgacatcac caccctcctg cagaacccgg ccgggtttcg gcgggcggtg
    16081 gaccagttgg aggggtacgt gcggggcgta caggcggaga gaatcctggc gatcgagtcg
    16141 cggggtttct tcttcggggc cgctctggcc gaccgcctgg ggctgtcgat tatcccggct
    16201 cgcaagccgg ggaaattgcc ctatacgacg gtgagcgagg agtacgaact ggagtacggg
    16261 accgaccggc tcgaggtgca tgcggatgcg gtcctgccgg gtgaccgagt ggtggtggtc
    16321 gatgatttga tcgccatcgg gggaacgctc ctggccgcct gccggctggt cgaacggctg
    16381 ggcggcgtcg tggccggggt ggcggcggtg atcgatctgc gcttcctccc gtggcgggag
    16441 aaactctccg ggtacgatgt ccgctacctg gtctcctatg actcggagtg agggggaaaa
    16501 ggtcgttgac aaagccgggc ggcgagggtt tcttgcgggg cggattgcgc gagacggcgc
    16561 ccccgtagct cagtcggata gagcagcggt ttcctaaacc gcgtgccgca ggttcgattc
    16621 ctgccggggg taccaaaaaa gacggcagga aaggggcaag gattgcgccg gaggaggtca
    16681 acccattcgc gaacatgcat ctagaccggg gacccggcgg ggaaatcgtt tgacaggcga
    16741 ttggggcagg cctatatttg ttggttaatt ttcggtcccg gtgcgtctgt agcagtggaa
    16801 gttagttgtg cgatacgata aggagtcgaa ggtaatgaag tcgttgacgg tgaagggcac
    16861 ggtggtgctg ctgctgatcg gaacaacggt gttcgcccag gggggcgtgg gcctggagta
    16921 cggaaatcgt ctggagttcg acgtctggga tttcagccgg cccgctctcg agggctacca
    16981 gatcgatttc agcggcggcg gcgcgcgcgc gctggggatg gggagagcgt tcctcgggct
    17041 gtcggatgac gtgaccgcga tcagctggaa cccggcggga ttgtacactc tcagccagcc
    17101 cgtcatctcg gccacctacg ggagcaccct gccgcgcggg tacaccgaca ccgacccgca
    17161 gttcgtgctc gatccgtcgc agacgcgctt cgaccacaac gggtggatca gcgggatcac
    17221 ggccctggat ttcgcggccc ccctccgcat caagggacac cccttcgtcg ggtcgttcac
    17281 ctactcgacg ggattcgacg agtaccaggg gtaccagttc tcgttcgaca ccacgttccg
    17341 cgacaccttc accaccccgg acacgatatt cgtggacacc gtggtgcact atacggtgaa
    17401 ccgggagtcg gagatggacg gggcgctgaa ggcggtgagt ttcggtttcg ggacgcggat
    17461 ttaccgcgag tggtcggtcg gcgcggccct caacgtgtac agcggaacgg cggtgcggca
    17521 cgcggcgagt tacgaatcct cccaggatcg catcgactac tcgttccagg aatactcgca
    17581 ggtgtatgcg acgaccgtga tcgactccaa ccggttttcg ggctacaact tcactctcgg
    17641 tgtgcgctac aacgccagcc cgctcgcggc cgggcttatc atccggaccc ccttcgacct
    17701 cgaagtggag actgaccggt cgatctacga gatcgccaag aagaacgagc gcgtggcggc
    17761 ccaggaaacc gataccttct atttcgacga catggtgacc aagtacgaga tgccctggat
    17821 gataggggcg ggcgtggcgt acaaggcaaa cgagcggacg gtgctgacgg cggatgtgga
    17881 gtaccggcgg ttcagcaccc agggcgtgct cctgcgcgac agcgtcaaaa tcgatccggg
    17941 cggcgagaac gaggagttct tcacgacggt cgcggccgac acgctgtgga aagacgtgct
    18001 gcaggtggcg gtgggggcgg agtacctctg ggaccagact tgggggacga ttccgctgcg
    18061 gttcgggttg gcctacgtgc cgacgattgt cccgagctac gagcgggatg agtcggacac
    18121 ggcgaacgcg gtcctggggc tggacagcgc ctcggtgacg ggccggctgg tggccaagca
    18181 ggagcggacc aacggggtgc gcctctcggc tggcgccggg atctggtgga agcaggtgca
    18241 ccttgatttc gcctattcgt acctgacccg cgactgggac accggggtgc ccgggacggt
    18301 gcgaaccggg gcgtttcacc agcgaatcag aaaccaccat gtgagcgcga cgtttacggg
    18361 gtacttctag agcggagaac ctcgagaggc ggcgcggtcg ggctcggtcg cgcccggaat
    18421 aagttgatcc taacgcggat ggcccggcag ccgataacga gtgtaaaacg gcgccggtaa
    18481 ccggccggag agaaacggat gcagagcaaa gtcaagctgt ccaagcggca gatcaaagaa
    18541 gacaaattca cggcgttcgt cctgacgtcg aaacagcggg tggccgaaaa ctggcagttt
    18601 cttgtgatcg gcggggtggt cctcgtgctc gcggtggtgg ccgtcgtcta cttcgcgagc
    18661 agccggacca gccgggaggc cgagggaagc cgattgttcg cccaggggat ggctgaatac
    18721 cgcaacggca accgccaggt ggcgctgacg acgttgcagg aagttgtgga cgagcacggc
    18781 gggcacgaga cggccgataa cgccacctac ttgctggggc agatccagtt tgaactgcgg
    18841 aatttcgccg aagccatccg gtggtgggag cagtacctgg ccaagtaccg tgacaaccgg
    18901 accgaccggg cggcggccct ggccgggatc gcggccgcca acgaggagca gggcaacaac
    18961 gcggctgcgg cccagaagta ccgggaagcg gcggccgagt atccggaggg cccgctggcc
    19021 tgggattacc atacgggggc gatgcgcaac tacctggcgg cgggccaggt tgacaaggcg
    19081 cgcgaggagc tcgactcgct gcgcgacaag tatgccggca cggagattta caaccgggcg
    19141 gcgcggctgt tcgccgcaca agcagtgccg gcgaaaacgg cgccctaagg ggcgtccggg
    19201 gccgggcggc cgtgggtatg ccgatcaaag ttgcgcttct ctggcacatg caccagccca
    19261 attaccagga gccgcagtcg cggcgcctgg tcctcccgtg ggtgcgcctc cacgccgtca
    19321 aagactacct cgacatgccg ctcctggcgg ccgaacagcc aaacatccgc gtaaccttca
    19381 acctcgtccc ctccctcctc gatcaatttc agctctacgt tgacggcggg gtcgaccgcc
    19441 atttggagct ctcgcgggtc ccctccgacc aactggacgc cgacctgcgg cgggagattc
    19501 tcaccacctt tttcgccggg cacccggcga cgatgatcga gccctacgcc cgctacgccg
    19561 aactctaccg caagtaccgg agcaacctcg gagagtcggt gctgccggcg ctgttcactt
    19621 cggaggaatt ccgggatctc caggtgtggt cgaatcttgc ctgggtggac ccgctctttc
    19681 gcgatgagga gccgatccga tcgcttttgg cgcgggggcg ctacttcacc gagggccaca
    19741 agcacgccct cctcgactgg caggtgcagt tcatcgcccg gatcgtgccc acctaccagc
    19801 gcctgttccg cgaagggcgg atcgaggtat catttacgcc ctattaccac ccgattctcc
    19861 ccctgctgtg cgacaccgac gtcgcgcgcg aggcgattcc gggaacgaat ctgccccgcc
    19921 gcttccggca tcccgaggat gccgagaagc agatcgtcat ggcgcaggag aagtaccggg
    19981 agctgttcgg gcgggagatg gccgggatgt ggccctcgga gggatcggtg tcggaggagg
    20041 tggcggcgct gtgcgccaag aacggcgtga gctggatcgc caccgacgag gaaatcctga
    20101 ccgcctcgct gaagaaatcc ggtctcgacc gggccgacaa tccgccccac gcggtctatc
    20161 agcacagcac gggcgtgcgc atgctcttcc gcgaccacgc gctctctgac cgggtggggt
    20221 tcgtctactc cggctgggag gcggagcgcg ccgtgcagga cttcgtcgcg cagatcaagg
    20281 agatcgggcg gcggacgctg gcggccgggg tcgagcccgt ggtgccggtg attctcgacg
    20341 gcgagaacgc ttgggagtat tatcccgccg atggccggga tttcctccgg ctgctctacc
    20401 atcagctggc gaccgatccg gatatacgga cggtgacgat cagcgaggcg accgaggggg
    20461 tggcggcgcg gccgctgccg aaactgttcg ccggctcgtg gatcaatcac gctttccgga
    20521 tctggatcgg gcacgaggaa gacaacgtcg cgtgggacct gctcagcgac gcgcgcgacg
    20581 cgctggtgaa ataccaggag gagcgcccgg cggccgaccc ggccgcgctg gcagcggcct
    20641 ggcggcaaat ctatatcgcc gagggatcgg actggtgctg gtggtttggc gatgagcacc
    20701 ggggattcga caacaaaact ttcgaccaca tcttccggcg gcacctcgtg gccgtctacc
    20761 gcctcctcgg gttggatgtg cccgaccgcc tgtgggaacc gatctaccag ggggagacca
    20821 agtcgcggac tgtcctcccc gacgctctgc tcacgccggc gatcgacggg cgggtgacgc
    20881 acttctacga atgggcgggg gccggcatgt acgactgcgc cgaggcgggg gggagcatgc
    20941 accgggtcga ccggctggtc tccgcgatct atttcgccta cgaccacgag cgtctctaca
    21001 ttcgggttga cttcgtcaat agaaaggcgc tactatcgat agtcgagccg gcggtcgagg
    21061 tggtgtttca ggcgccgcaa ccggtgcggc tcaacgcgcc ggtggcccac ggcgggcgcc
    21121 gggtggacgc cgagaaaggg tacgaggtgg
//
LOCUS       DAOPOE010000054        21047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3113_length_21047_cov_5.895722, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000054 DAOPOE010000000
VERSION     DAOPOE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_21047_cov_5.895722"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKM94_08440"
     CDS             <1..518
                     /locus_tag="PKM94_08440"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOD66703.1"
                     /translation="VSQIARFLTGVEIHPGATIGPGFFVDHGMGVVIGETTEIGRNCV
                     LFHNVTLGGTGKHQGKRHPTLGDNVFVGTGAILLGPITVGENVRIGANSFIVMHDVPA
                     NTTVAGVPARIVRRGRQKVDEELPATVLDHGRSVHTEIGEHNNRNGRKPGIREVRTDG
                     SAPPERAASGS"
     gene            complement(571..978)
                     /locus_tag="PKM94_08445"
     CDS             complement(571..978)
                     /locus_tag="PKM94_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66704.1"
                     /translation="MRPLSDWIAHASPYRLDSLFLPRQPLKPHVVLPPVVIPAGKLDF
                     FFDTACVEGNLVLRHDGRVELTDITESHPLQEFADYVRAAVAMAMCYPMRDADGVPVT
                     VTMRYRCIITHDGSPQVLTDDTSIVATQRSLDE"
     gene            complement(1753..2556)
                     /locus_tag="PKM94_08450"
     CDS             complement(1753..2556)
                     /locus_tag="PKM94_08450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD66705.1"
                     /translation="MDLLGDPTVADHPYSLPHLYDVAFSFRDYARAVDFLLAAARRAS
                     GRDPASVVELGCGPGQYCREFARRGLPAFGVDRAPEMAAYAARLAAGENLPCAILEAD
                     IRRFTLPHPVDLACCMMATFHLLLTDDDVAAHLDAVADNLNPGGIYIIELSHPRDFLT
                     RQRSTRNLWTVDRDGLSVTVDWGSDAVTDPLSEITTGTVSYAVSGPSGAARHESREQW
                     REISAGLIRALIRLSGRFAIVAWYGDLDTGVPFDAGDRAWRMILVLRLC"
     gene            2610..3662
                     /gene="purM"
                     /locus_tag="PKM94_08455"
     CDS             2610..3662
                     /gene="purM"
                     /locus_tag="PKM94_08455"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOD66706.1"
                     /translation="MTAKTPKKKLTYADAGVDIAAGDEAVARIKALARGTFNAQVLSE
                     IGAFGGFFRPDFQGMTAPVLVASADGVGTKLKLAFMTGKHDTVGEDLVNHCVNDILVH
                     GARALFFLDYIAVARLEPHVVADIVAGLARGCRNAGAALIGGETAELPDFYAPGEYDL
                     AGFIVGAVEEARIINGASIRPGMVCLGLPSSGLHTNGFSLARKAAFEIAGRRPGDRIE
                     ELGTTVAEALLAVHKSYAPVVHPLLPQFPIHGMAHITGGGLPGNLNRVLPKDCDAWVR
                     KGSWPVPPVFDWLGRAGDLDREDMYSAFNMGIGFVVVVEAEQADQVVRTIERLGEKAY
                     RIGEIRKGEGKVRLTD"
     gene            3809..5038
                     /gene="apgM"
                     /locus_tag="PKM94_08460"
     CDS             3809..5038
                     /gene="apgM"
                     /locus_tag="PKM94_08460"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD66707.1"
                     /translation="MVKRVVLLVCDGLADRPIKEFGYKSPLEAARTPHLDRLAKESVC
                     GMVHTLGRGRVPGSDVAHLEIFGYPIEKYYAGRGPIEVCGLGMKLQGGDVALRGNFAT
                     VDKDWNIVDRRAGRINHVAPLCAAIDGIEIDGVKFLVHPGTGHRAGVIMRGEGLSDKI
                     IDADPHGPGEPVRTVTATDASPEARRTAEVLNRFLRKSYEALGGLDLNRQRVKEGKLP
                     ANILLVRGAGQHIHAPSFSERYGLKACAIAGGGLYKGVGSFVGMDVVEVPGATGLPDT
                     DIAAKFRAVLAKLAEYDFVFVHVKAADSLGEDGNAPGKKDFVEKIDAAAKILAEGLPE
                     DVLLVATADHTTPCEMKAHAADPVPIMFRGNNVRVDGVAAFGERACAQGGLGFMLGRE
                     IMPQVVNFLGKGHLEGA"
     gene            5628..8333
                     /locus_tag="PKM94_08465"
     CDS             5628..8333
                     /locus_tag="PKM94_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOD66708.1"
                     /translation="MSESRSYATIRAAGLLLAAAIGAALLASSVPATVATPEEAARVS
                     QNWLTYMVYQQGNWAGEKAPQLLGSEEITFQGMVVGYHFRVAPSGYIVVPVLKELPPI
                     KAYSETDVLNVKNRQGMGGLLREVLYQRIAEYIKVYGSADAAQPATGDVFLDRSHGRE
                     WEMFLQSEASLTAELYSGRAGRQVTVGPLLASTWHQTGPYNSMCPVGDAACLDCADPG
                     EDPSPPSSDTAVVGCVATAAAQIMYFWNWPPSGTGSYSYTWDGDQTCGQPAGSTPAQT
                     LSANFSDAYDWGNMRDSYTGGESQAQRDAAAELCYEAAVAAGMDFGVCGSGTSTDTVL
                     TAFPMYFRYSAATIDKENRTDHTADEWFQLLAAEVDAGRPVQYRINSHSIVCDGYRDT
                     PSDQVHMNYGWGGGANTWYAVDNFYCYWIAGEICPWDEEYMIRGIQPDNLPPVAQCAD
                     VTVDADDNCQAAAASIDNGSYDPEGYAVTLEQIPAAPYPLGATLVTLRVTDPQGGVAT
                     CEATVTVVDVTPPIITCPSDRTFECDDIGDFGWPTATDNCDSDPAITLASRDSVPGGC
                     PQEYRLVLGYKAEDFSGNAAGCSQTITIIDTTSPVVDCYDTLGFVFTSPTEAEVFWDA
                     SATDNCSPAPTVTCDPPSGTTFPIGEHTVTCIADDGCANTDTCAFTFRLVPFDLHPTS
                     CPNPIAVKSVVPAAILGTSDFDVLDVDPSSLRARGVAPLRWTYEDVSTPVGDDPNQNC
                     ECTRAKGDGRKDLTLKFDHAALVATFAPGELVDGGSVMLQITGLLKDGTPLAGGDCLI
                     LRYKPKDTPAESGPVAVTELGAWNSPNPFNPATVIGYQLPEPGHVKITVFNMLGQVVA
                     HLIDQWQEAGQHSVSWSGERAASGVYFYRLEVGEAQLTRKMLLLK"
     gene            8566..10164
                     /gene="lnt"
                     /locus_tag="PKM94_08470"
     CDS             8566..10164
                     /gene="lnt"
                     /locus_tag="PKM94_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOD66709.1"
                     /translation="MALATRIWRVILPDDVNVRRRRLELTVWAFVLSLAFYPGPWGFL
                     AWVALVRPIMIFARLGGKAAFRSAYLFGFLFNLFSLYWVALVTPPGMITAVAIVALYY
                     AVVLCLFVRLHRWKAAAGYVALPLVWVGMEYFRTLSEFAFPWSDLGYTQAHYLWVLQI
                     VSVIGVHGLSLLIVAVNVLLAQVFRPSLSYERRVGALFGAAATVGLVTAYGWVVLPPH
                     PKPGTFGVALLQGSVPLDVKWSPDSVDENFALYERLTASVADSAVKLFVWPETAAPCY
                     ITHAPECQAAVGRIAAESGTPHLVGTLAARMVGGKMRHYNSAVQFDGQGVLTAQYDKV
                     KLVPFSEHVPYQDHLRFLEKDFLRKYLTFIDEGGVQWWSDFYPGDSLRLFAVGDIQYA
                     ALICFEVAFPEYVRAMVRRGANFLVEITNDTWFERSVGVHMHSRVFITRAVENRSWGA
                     RAANSGLTYIVDDYGRIREGLDLYAVQVLAGKVGLLYNYSVFTRIGDVAGRGSFLLTA
                     GIICIFIALWCRQKLSRFSSRARS"
     gene            10116..12341
                     /locus_tag="PKM94_08475"
     CDS             10116..12341
                     /locus_tag="PKM94_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66710.1"
                     /translation="MVPAKTLAVFLAGAVLIPAALPGGAAAAGESWTTLTSFKTVRAF
                     AVVGDTLYAATPGGLLLIADSSSPGRAYLHADGLGTNDLEDVLVDDAGTVWLAGLGHL
                     IRFDRPAPRQFLFVDRDGQAIRLQALADDGDRLWVGVHGGLVLFSKSDDGGQIEDSYP
                     MFGTLTPSPAVVDVALVNDTLWLATSAGLAAAPAASPVLLKTPGTWTTFAAARYPELG
                     RDSIATVEAFGGEIYAATSAGAFRLNRDGADTSFVPVALTAGRAVTDLVAEGDSLFIY
                     HSGGLAVVTAGGAADLPRAGLSLPPTTGIGWEGLRWVGMRDSGLFVGAAGQYREYPHT
                     GAPGNDVRTILFGPEGRITAGFGDKIFAQYDGDRWMRRIFYRRGDGTMDLLADGRGDI
                     WAASWGGGLFRIRGDSITQYTDSNSTVVGVVAGDPLEYTVVRSLATDGVNIFASLFQA
                     YNKDVVAWAPLDRVHDEAAWGALGVPEGLANEYVTALAYADGELAVGTMARGVYLCRL
                     GSAPYRGTAITVRHFTDDNSRLISNAIRTVAYAPNGEAWIGTNLGISRWDSGIERLVD
                     VVLPVGVGTDVAAIVFDGRGNAWVGTRGGLVRLDATGGAPDIFTAATSDLVSDEIRDL
                     ALDRRSGDLYIATAGGVSVLRTPIENFVETVGDSVVPIPNPFVIRSAADRLRFNLDRP
                     ATVSIYTAAGERVAEFPVNEPWDGRNDRGRAVASGAYVYVITDETGASHRGKILLVRK
                     E"
     gene            12338..13261
                     /locus_tag="PKM94_08480"
     CDS             12338..13261
                     /locus_tag="PKM94_08480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66711.1"
                     /translation="MKAICYHDDDMPEEPVRALTGDSFLASAAFASLWAVRGGRRAVW
                     AAQAGEGIAGVLPGIEFGPAPIKRFMAMPNGLYGRVFAAEGSDPRKTGEVLLEAIAEA
                     GYVKTYLFDFFGCFTSGTGPFAVALQTALLVDIGDPAWEPPDRKLRGEIRKAEREGLA
                     VGRMVWATHGEGFLTLLRGKAHRRGGEARYGADFWQALAHAAQQDERVAWYYASFNNR
                     PVASHIYFVEGDMLIGWQMYYDRKFSPLKANAYLIARACAEARDRGARTLDLGATPEG
                     AGGTEFFKRRWGGREHIYPTLTLRRGIGRWL"
     gene            13252..14499
                     /locus_tag="PKM94_08485"
     CDS             13252..14499
                     /locus_tag="PKM94_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD66712.1"
                     /translation="MAVTGAHLRVLVLADARSVHTQRFAAELRRQGCAVLVASLEGSP
                     VDPALGGAEEDPKRTAAPEAAGAPGEPEEGCGASVRLRRRFPIRTVAYAWAAREVERL
                     IERWRPEAISAHYAAGYGYLLSRVRRRPPATLHVWGSDVLVVPHRSRLHLRKVVRGLR
                     AADHIFADSRYLAEEAGRLAGVTNVTVHPWGIERRHLQRRLRVGSIGRPLRIIVPRMH
                     ETVYDNEFVLEALREMIIRGEATVTFPAFGSRYERFRRRAEGMGLRVVSLEAGAARDG
                     TGTEADPRGAVCCYEKRNRDGFLALMAAHDVYVSAARSDSSPATLIEAMALGLAPVAA
                     DIPGVREWLTPESGFAFRPGDAESLRTILARLATGEAGGAECALDGLRRRNREQVERE
                     AIFEDNIAAQLAVMRALCGKAGH"
     gene            14499..15752
                     /locus_tag="PKM94_08490"
     CDS             14499..15752
                     /locus_tag="PKM94_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66713.1"
                     /translation="MRRALLVCYYFPPLSGAGVARPLALFKHLPEFGWECHVLSVKPV
                     AYRAFEPELLDGLPGRHIYRAGSSDPQRLMRLMGIRTVADRVIRKGRALADWYFPDPK
                     IGWVRRATALGRTLLANRDYDAIISTSPPVSAHLVARTLSREFKRPWIADFRDYWTIH
                     RPEQLYANRPRRRRRAQRLLAAIRDEASEVTAVNETVREYVGAGTVISNCYDEALAAG
                     WTLPAGSPFVIGVLGALNEITPVAPLLRLLARLRRERPEVFPRVRVRQVGEVDGQWLE
                     RQIDACGVQEIFDRYGWQPRRETIAILSEASLMYLGVASAEERGLSTGRVYTMLASGR
                     PILAWAPEDSELARLLAHTGSGVCFFGSDDSPAVKYLADLVQAHGEGKTPVTLRPAYA
                     EPYSSRRMTEKFAALLDRQKPQSYR"
     gene            16034..16510
                     /gene="mraZ"
                     /locus_tag="PKM94_08495"
     CDS             16034..16510
                     /gene="mraZ"
                     /locus_tag="PKM94_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOD66714.1"
                     /translation="MNGFVGRYNTTLDEKGRFALPAKLRSILGAGGKPQLDGALILTK
                     GLEGCLSLYTEPEWNDIEKRLSSLSFTQRDFRYFGRRFFSMAAWVTPDRSGRILVPTH
                     LIAEADLKKELLLIGVNKWIEVWNPERYEDYLRGYEATYEDVAERLFPRPSGDDRT"
     gene            16497..17441
                     /gene="rsmH"
                     /locus_tag="PKM94_08500"
     CDS             16497..17441
                     /gene="rsmH"
                     /locus_tag="PKM94_08500"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOD66715.1"
                     /translation="MTGPEPIHVPVLRDAAVRLWAARRDGAYLDLTVGAGGHLKALSD
                     YLDGTARLYGIDRDPAAAAAAAAQLAGAPQTVTIARGAYGEFETVTAAFGDRTYDGML
                     MDLGLSSLQLDDPRRGFTFRHDGPLDMRFDPESGGPTAADLVNSLGEKELVDLLHGYG
                     EERRAKRVAQAIVRERRMRMVQTTSQLAGLVERIAPARDLHKTLARVFQAFRIAVNGE
                     LEQLAATMPRLLPRLRAGGRLVVISYHSLEDRLVKRFFQAEAKGCVCPPEAPLCACGR
                     APRLRILTRRAVVPDETEAAANPRARSAKLRAAERVGA"
     gene            17450..17932
                     /locus_tag="PKM94_08505"
     CDS             17450..17932
                     /locus_tag="PKM94_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66716.1"
                     /translation="MRKFKETVEIRSSLLVRVRSHRYFPIGAVAGVFLLAACVHIWQR
                     VHVIHLAQEIAVLRMENRLLGDDTRKVHADLSALSMSSRVETYAADSLGLAQVAATEL
                     ITLVPEGVQAPSVKDDLHMVVKMAERWANVLPSVTQAQANAGELISPVIDSLANGGDD
                     "
     gene            17932..20010
                     /locus_tag="PKM94_08510"
     CDS             17932..20010
                     /locus_tag="PKM94_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOD66717.1"
                     /translation="MHRTRTERIRLGILFSLVCLYFLAVAARLVHLQVVLHQKYADIV
                     RIQSEGRVKIPAERGLIYDRYGRLVVNNVKCPSLYAYPGSQTELENAARYVEKMFGLK
                     RGTARREYNLEVNKFSWIKRLLGDEFARHIEKTAPPGLYLRDETQRVYPYGLVGKQIL
                     GFTNIDNQGQSGLELAWDSLMKGEDGEADIRRDGLRNVFRVKEQAAVKPKPGQALVLT
                     IDFNLQEIFERQLRKGVEEYGAKAGMGAFIDCRTGEILAMAHYDPKEEHPEKPVKLRA
                     ISDQFEPGSSFKAITAAALLEAGEVDFREYTYCEMGAWKMERGGTLKDDKKHGNLTFR
                     EIMELSSNIGVAKYGIRLGGENLYEAARKFGFGQKYGIGLGAEESGRVSKPRRWADRA
                     VASLAMGHAVAVNCLQMANAFAAIANGGELLRPHLLMCCVDEHQKVTRRFGREVMQKV
                     MEGVWADTLKAVLRGVVERGTATKVNSKAVAIAGKTGTAQIPDSVTHRYKDEYTASFA
                     GFFPYEKPAIAGIVMLIEPNPIHYGGYTAGPIFRRTAEQYMILNPDLFDSGNQLLAER
                     SSSREGTVEAPNLVGRSLADAAEDAEERGLTIRANRTEGMIVWQYPPADRLMFAEDDL
                     IVVVESPVETGFVMADLTGLSIREASAFLDFAGIKCRITGRGKVISQSIGPGQAAGSE
                     DVCQLECRPT"
     gene            20038..>21047
                     /locus_tag="PKM94_08515"
     CDS             20038..>21047
                     /locus_tag="PKM94_08515"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOD66718.1"
                     /translation="MHGCAGAVITGDPEVAIEHLEYDSRLVRPGTLFFAVTGFTRDGY
                     DFVADVLSRGAVAVMGERESHPAVPVHVRVPDIRQAMADAAAKFYGFPGKQLFAIAVT
                     GTNGKTTTCYLLRCMLEAAGRKAGLVSSTLYDVGGEQFPAERTTPEALDLQRLLFLMR
                     KNGCAAAVMEVSSHALALKRVEHILFRVGVFTNITRDHLDFHKSMAEYLAAKKRLLGK
                     LAGPHACAVLNLDVPEFRAFFGDARSEHMSYSLQDTHADVYASRYEIEPMRTVFDLAT
                     PAGMRTVTFPLPGRFNLMNAVAAAAGGLAAGIDLDTVVAGLESATPVPGRFNAVDAGQ
                     PFG"
BASE COUNT         3540 a         6425 c         7625 g         3457 t
ORIGIN      
        1 ttgtttccca gatcgcccgg tttttgaccg gcgtggagat ccatccgggg gcgaccatcg
       61 ggccggggtt ctttgtcgac cacggaatgg gcgtggtcat cggcgagacg accgagatcg
      121 ggcggaactg cgtcctgttt cataatgtaa cactgggcgg aacgggcaaa caccagggca
      181 agcgccatcc gacgctcgga gacaacgtct ttgtcgggac cggggcgatt ctgctcggcc
      241 cgatcacggt cggtgaaaat gtccgcatcg gggcgaacag ctttatcgtg atgcacgatg
      301 tgccagcgaa taccacggtc gccggagtcc cggccaggat tgtccggcgc gggcggcaga
      361 aagtggatga ggagctgccg gcgacagtgc tcgatcacgg gcggagcgtc cacacggaga
      421 tcggcgagca caacaaccgc aacgggcgga aaccgggcat acgggaagta cggaccgacg
      481 gctccgcgcc gccggaacgg gcggcctcgg gaagttaggt tcccgcaaga cacgggcaac
      541 gcgcccggcg cccggcagcc tgctggccca tcactcgtca aggctgcgct gggtcgccac
      601 aatcgatgtg tcgtcagtca gcacctgagg cgacccgtcg tgagtaatga tgcaccgata
      661 gcgcatcgtt acggtgacag gcactccatc ggcatctctc atcgggtagc acattgccat
      721 ggcaacggcc gcccgcacat agtcggcaaa ctcctggagg ggatgggatt ccgtaatgtc
      781 tgtcagctcg acccggccgt catgccgaag gaccaggttc ccctccacgc aggcggtgtc
      841 gaagaagaag tcgagcttgc cggccgggat caccaccggg gggaggacga catgcggttt
      901 gaggggctgt cgcggcagga acagtgaatc gagccggtac ggcgaagcgt gcgcgatcca
      961 gtccgagagc ggccgcattc caggatacgc atcttggtcg cccaagccca ggccgtacac
     1021 aacctcaccc gtatccgccc cagagttggg ggagccggca acaaattcag gtccatactg
     1081 gccgaccctg gggacgtctg catgttgagg tcgcgggcct gagctgtccg gtgccacgcg
     1141 aaccttgccg acataccccc ggtaccaatc ggcatcaccc gcagcacctg attgtgcgca
     1201 agcggattgg agagggggaa accgggcggc actcccttgg ccggcagcca ccggatcctc
     1261 ggccgtggtc ggcgacggcg ctatggccag caggagcagg atggcggccg ccgccgcatg
     1321 aaccgaggca gccgcgagga gttgaatctg atgctgtcgt ccgcggtgat cggtaatccc
     1381 tcgaatcgcg ctcggtatgc gatatgtgct ttcagagctt gtccgcataa ggcacccccg
     1441 tgagcttgga atagtatagt cagcggttgc gacggcccgc aacagtcaat ctcgcgcccg
     1501 cgaatttggc cgagctcctt aaggcagacg ccgcggcgga ggagtcaggg ggcggcagcg
     1561 acaaccgcac ggggagtgcg gagaggacgg cggcggcctt ttcgcgcata accggccggg
     1621 aatggcgttg gcctcgatca ctcgcggctc acgcgcggac cgcctcgcgg tctcagtatc
     1681 tacggcgggt tcgcgcagga aagcagtgtg ccggtccggc cctcctccga taggcaggca
     1741 cgaaggcggg cgtcaacaca ggcgcaggac gaggatcatt cgccaggcgc ggtcgccggc
     1801 gtcgaatggg acaccggtgt cgagatcgcc ataccaggcg acaatggcga accggccgga
     1861 gagccttatg agcgcacgaa tgagaccggc cgaaatctcc cgccactgct cgcgggattc
     1921 atgacgcgcc gcgccggagg gcccggagac ggcgtacgaa acggtgccgg tcgtgatttc
     1981 ggaaagcgga tccgtgaccg catcgcttcc ccaatcgacg gtgacggaga ggccgtcgcg
     2041 gtcgacggtc cagaggttgc gggtgcttcg ctgccgagtg agaaagtcgc gcgggtggct
     2101 caactcgatt atgtagatgc cgcccgggtt gaggttgtcg gccaccgcgt ccaggtgggc
     2161 ggccacgtcg tcgtcggtca ggagcaggtg gaaggtggcc atcatgcagc aggccaggtc
     2221 aacgggatgg gggagggtga agcggcgtat gtcggcctcg aggatggcgc acgggaggtt
     2281 ctccccggcc gccaggcggg cggcgtaggc agccatctcc ggcgcgcggt cgacgccgaa
     2341 ggccgggagg ccgcgacggg cgaattcgcg gcagtactgg ccgggaccgc agccgagttc
     2401 gacaacggaa gcgggatcgc gcccgctcgc gcggcgggcc gccgccagca gaaaatcgac
     2461 tgcgcgggca tagtcgcgaa aactgaaagc gacatcgtag agatgcggga gagagtaagg
     2521 atgatcggcc acggtcgggt ctccaaggag gtccatccgg gacatccggc ggacgttgac
     2581 atttgcatac cccttccgta tatagcgcca tgacagcgaa gacacccaag aaaaaactga
     2641 cctacgcgga cgcgggcgtg gacattgcgg ccggggacga agcggtggcg cggatcaagg
     2701 cgctggcgcg cgggacgttc aacgcacagg tgctgagcga aatcggcgca ttcgggggat
     2761 tcttccgccc cgacttccag ggaatgaccg cgccggtgct ggtcgcgtcg gccgacggcg
     2821 tggggaccaa gctgaaactc gcgttcatga ccgggaaaca tgacacggtc ggcgaggatc
     2881 tggtcaatca ctgcgtcaac gatatcctcg tccatggggc gcgggcgctg ttctttctcg
     2941 actacatcgc cgtggcgcgt ctcgagccgc acgtggttgc cgatatcgtg gccggcctgg
     3001 cgcggggctg ccgcaacgcc ggggcggcgc tgatcggcgg ggaaacggcg gagctgccgg
     3061 atttctacgc gccgggggag tacgacctcg ccgggttcat cgtcggcgcc gtcgaggagg
     3121 cgaggatcat caacggcgcc tccattcggc cggggatggt ttgtctcggg ctcccctcga
     3181 gcggcctgca caccaacggg ttcagcctcg cccgcaaggc ggcgtttgag atcgccgggc
     3241 gccgcccggg cgaccggatc gaggagctgg ggaccaccgt ggcggaagcg ctcctggccg
     3301 tgcacaaatc gtacgcgccg gtcgtgcacc cgctgctgcc ccagttccct attcacggca
     3361 tggcccacat caccgggggc ggcctgccgg ggaatctcaa ccgcgtcctg ccgaaagact
     3421 gcgatgcctg ggtgcggaaa ggttcctggc cggtgccgcc ggtgttcgac tggctgggca
     3481 gagcaggcga cctcgaccgg gaggacatgt actcggcctt caatatgggg atcgggttcg
     3541 tcgtcgtggt ggaggcggag caggcggacc aagtcgtacg cacgatcgag cggttgggag
     3601 agaaagccta ccggatcggg gagatccgaa agggggaggg gaaggtgagg ctcactgact
     3661 gattaagctc cctgcggctc cgcgccgagc gaccgcatcg cagggccggg ggcagggcgc
     3721 gggaaaggat cgcttccggg cggctgagcc atgacggaaa cacaggatca gttctgagac
     3781 agcgacgtga gacggagata tcaggaagat ggtcaagaga gtggtgctgc tcgtgtgcga
     3841 cggtctggcg gatcgtccca ttaaggagtt cggatacaag agccctctgg aggcggcccg
     3901 gacgccgcac ctggaccggc tggcgaaaga gtcggtgtgc gggatggtcc acacgctcgg
     3961 gcgcgggcgg gtgccgggat cggacgtggc gcacctggag attttcggtt acccgatcga
     4021 gaagtactat gccgggcggg ggccgatcga ggtgtgcggg ctgggcatga aactccaggg
     4081 gggagacgtg gcgctgcggg ggaattttgc gacggtcgac aaggactgga atatcgtgga
     4141 tcgccgcgcc ggacggatca accacgtggc gccgctgtgc gcggcgatcg acgggattga
     4201 gatcgacggc gtgaagttcc tggtgcatcc gggaacaggc caccgggccg gagtgatcat
     4261 gcggggcgag gggctgtcgg ataagattat cgacgccgac ccgcacgggc cgggcgagcc
     4321 ggtgcgcacg gtgaccgcaa ccgacgccag cccggaggcc cggcggacgg ccgaggtgct
     4381 caacaggttc ctccgtaaat cgtacgaggc gctcggcgga ctggacctca accggcagcg
     4441 cgtgaaggag gggaagctcc cggccaacat cctcctcgtg cgcggcgcgg ggcagcacat
     4501 ccacgccccg agtttctccg aacgttacgg tcttaaagcc tgcgcgatcg ccggcggcgg
     4561 gctgtacaag ggtgtcgggt cgtttgtcgg gatggatgtc gtcgaggtgc cgggggcgac
     4621 cgggctgccg gacacggata tcgcggccaa gtttcgcgcg gtcctggcca agctcgccga
     4681 gtatgatttc gtgttcgtgc acgtgaaggc ggccgattcg ctcggcgagg acggcaacgc
     4741 gccggggaag aaagattttg tcgagaagat cgacgcggcg gcgaagatcc tcgcggaggg
     4801 cctgccggag gacgtgctgc tggtggcgac ggccgaccac accacgccgt gcgagatgaa
     4861 ggcccacgcg gcggacccgg tgccgatcat gtttcgcgga aacaacgtcc gggtcgacgg
     4921 cgtcgcggcc ttcggcgaac gggcctgcgc ccaaggggga ctgggtttca tgctcgggcg
     4981 ggaaatcatg ccgcaggtgg tcaatttcct cggcaaagga cacctcgaag gagcctgagc
     5041 cgggtccggg cgcccggcga agcagcggag aaggagagaa tcggcgggct gcgaccccgg
     5101 acggtcccgc ctccctcccg accggaacac ctgccggagc gccccgccat cgcaaatgtc
     5161 cttgacaggc agtccccgct cgccgcagat taggaccata cgaacgcaga gttgatcggc
     5221 gcggccgtgc cgggcgagac ggcattaccg ttcggctgcg ccgggagtca gggctgtgag
     5281 ggtccccatt accggcagca ggtgctcttt ggccgatcgg gcggtccgcc cgcttgggcg
     5341 gcatgtcgct ccgaagtgtg cgccgggtgt gggtgaccgc gggcgagcaa cagtgaacac
     5401 gcagggtgat tatcatcgaa cggcggcctg cgcgatgcgg cgcgcggcgg atttgaggcg
     5461 ccgccgggca attccgcatc gctgcactcg tcggagaagc ggccgatgcc ggtccccaac
     5521 cggcgaagga ccaagcactt acatacctga actgtttgac ccaactgacc gtcaatcgat
     5581 atggtttccc gaggcaaagc cgtcggggga caaagggagg tgctatgatg tcggaatcca
     5641 ggtcgtatgc gaccatccgc gcggccgggt tgctgctggc ggcggccatc ggcgccgcac
     5701 tgctggcgtc ctcggtgccg gccacggtgg cgacaccgga ggaagcggcc cgggtcagcc
     5761 agaactggct cacctacatg gtgtaccagc agggcaactg ggcgggcgaa aaagcgccgc
     5821 aactactggg gagcgaagaa atcacattcc agggtatggt ggtaggctac catttccgcg
     5881 tcgctccatc cgggtatatc gtggtccctg ttctgaaaga gctgccgccg atcaaggcgt
     5941 actccgagac cgacgtactc aacgtgaaga atcggcaggg gatgggggga ctgctgcgcg
     6001 aggtgctgta ccagcgcatc gcggagtata tcaaagtgta cgggagcgcc gatgcggccc
     6061 agccggccac cggcgacgtc ttcttggacc gcagtcacgg acgggagtgg gagatgtttc
     6121 tgcagagcga ggcgagtctg accgccgaac tgtactcggg gcgggccggg cggcaggtga
     6181 cggtcggtcc gctgctggcc tcgacctggc accagaccgg accgtacaac agcatgtgcc
     6241 cggtcgggga tgccgcctgc ctggactgcg ccgacccggg tgaggacccc tcgcccccgt
     6301 cgtcggacac cgccgtcgtc ggttgcgtgg cgacggctgc ggcccagatc atgtacttct
     6361 ggaactggcc gccctcgggc acgggttcgt acagctatac ctgggatggc gatcagacgt
     6421 gcgggcagcc ggcgggcagc acgccggccc agacgctgtc ggccaatttc tccgatgcgt
     6481 acgactgggg gaacatgcgc gacagctaca cgggggggga gagccaggcc cagagagacg
     6541 ccgccgccga gctctgctat gaggcggcgg tcgcggcggg gatggatttc ggcgtctgcg
     6601 ggtccggcac cagcaccgac actgtcctga ccgccttccc catgtacttt cgctacagtg
     6661 cggccaccat cgacaaggag aaccgaaccg atcacactgc ggacgagtgg ttccagctgc
     6721 tggccgcgga ggtggatgcg ggccgccccg tgcagtaccg gatcaacagc cattcgattg
     6781 tctgtgacgg ctaccgcgat acgccctccg accaggtgca catgaactac ggctggggcg
     6841 ggggcgccaa tacctggtac gcggtcgaca atttctactg ctactggatc gcgggcgaga
     6901 tctgcccgtg ggatgaggag tacatgatca ggggcatcca gcccgacaac ctgccgccgg
     6961 tggcgcagtg cgcggacgtc acggtcgacg ccgacgacaa ttgccaggcg gcggcggcgt
     7021 cgatcgacaa cggctcctac gaccccgagg ggtacgcggt cacgctcgaa cagattccgg
     7081 ccgctccgta cccgctgggg gcaaccctgg tcacgctccg ggtcactgac ccccagggcg
     7141 gcgtggcgac ctgtgaggcg acggtgacgg tggtggatgt gacgccgccg attatcacct
     7201 gcccctccga ccggacgttc gagtgcgacg atatcggcga cttcgggtgg ccgacagcga
     7261 ccgacaattg cgattcggat ccggccatca ccctggcgtc gcgcgattcc gtgcccgggg
     7321 gctgcccgca ggagtaccgg ctggtgctcg gatacaaggc cgaggatttc tcgggcaacg
     7381 cggccgggtg cagccagacg attacgatca tcgacacgac ctcgccggta gtggattgtt
     7441 acgacacgct ggggtttgtt ttcacgtcgc cgacggaagc ggaggtgttc tgggatgcga
     7501 gcgctacgga taactgcagc ccggcgccga ccgtgacgtg cgacccgcca tctggcacga
     7561 ctttcccgat cggcgagcac acggtcacct gcatcgcgga tgacgggtgc gcgaacaccg
     7621 acacctgcgc gttcacgttc cggctggtgc cgttcgatct ccacccgacc tcatgcccca
     7681 acccgatcgc ggtgaaatcg gtggtgccgg cagcgatcct cggcacgtcg gatttcgatg
     7741 ttctcgacgt tgatccgtca tcgctgcggg caaggggggt ggcgccgctg cgctggacgt
     7801 atgaggatgt gtcgacgccg gtgggcgacg accccaacca gaactgcgaa tgcacgcggg
     7861 cgaaggggga cggacggaag gatctgacgc tcaagttcga ccacgccgcg ctcgtggcga
     7921 cttttgcgcc cggcgagctg gtggacggcg gcagcgtcat gctccagatc accggtctcc
     7981 tgaaggacgg aacgccgctg gccggcggcg actgcctgat cctccggtac aaaccgaaag
     8041 acacgcccgc ggagtccggc ccggtggcgg tgaccgagct gggggcgtgg aacagcccga
     8101 acccgtttaa cccggcgacc gtgatcgggt accaattgcc ggaaccgggg catgtgaaga
     8161 tcaccgtctt caacatgctc ggccaggtcg tggcgcacct gatcgaccag tggcaggagg
     8221 cgggacagca ctcggtctcc tggtccggcg agcgcgccgc ctcaggtgtc tacttctacc
     8281 gcctggaagt cggggaggcc caattgacca ggaagatgct cctgctgaag tagccgtttc
     8341 gtttcacgcc gcaggatacc gcagcccccg gccgggcgat cgccggccgg gggtctttcg
     8401 cggtccgatc gggcgcgctc gcaccggccg actcctctta aaggcttgtg ccgggaggac
     8461 cggcagggta ctttctcccg tacttcggcc cgccggcgga gggaagccgc cggtcaccgc
     8521 gacccgaccg gtgaacgggc ggtccgaaca aaccggagat cggcaatggc actggccacg
     8581 cgcatatggc gggtgatcct gcccgacgac gtcaacgttc gccggcggcg gctggaactg
     8641 acggtgtggg cgtttgtcct ctcgctggcg ttctaccccg ggccgtgggg gtttctcgcc
     8701 tgggtggcgc tggtgcggcc gatcatgatt ttcgcccgac tgggaggaaa agcggccttc
     8761 cgctcggcct acctgttcgg gttcctgttc aatctcttct cgctgtactg ggtggcgctg
     8821 gtgacgccgc cggggatgat cacggcagtg gccatcgtcg ccctctacta cgccgtcgtg
     8881 ctgtgcctgt ttgttcggct gcaccgctgg aaagcggcgg ccgggtatgt cgcgctgccg
     8941 ctggtttggg tggggatgga atatttccgg acgctgtcgg agttcgcctt tccctggtcg
     9001 gacctcgggt acacgcaggc gcactatctc tgggttctgc agatcgtgtc ggtgatcggg
     9061 gtgcacgggc tgtcgctctt gatcgtggcc gtgaatgtgc tcctcgcgca ggtgttccgg
     9121 ccgtcgctct cctacgagcg ccgggtcggg gcgctcttcg gcgcggcggc gaccgtgggt
     9181 ctggtcacgg cctacgggtg ggtcgtgctg ccgccccatc cgaagccggg gacgttcggg
     9241 gtggcgctcc tgcaggggtc ggtgccgctc gatgtcaagt ggtcgccgga ctccgtggat
     9301 gagaacttcg ccctctacga gcggctgacg gcgagcgtcg ccgacagcgc ggtcaagctg
     9361 tttgtctggc ccgagaccgc ggcgccctgc tatatcacgc acgccccgga gtgccaggcg
     9421 gccgtggggc ggatcgccgc agagtcgggg accccccatc tggtgggaac gctcgcggcg
     9481 cgcatggtgg ggggaaaaat gcggcactac aactcggccg tgcagtttga cggccagggc
     9541 gtcctcactg cgcagtacga caaagtcaaa ctcgtgccgt tctccgaaca cgtaccctac
     9601 caggaccacc tgcggtttct ggagaaggat ttcctgcgca agtacctcac gttcatcgac
     9661 gagggaggag tgcagtggtg gtccgatttc tacccgggcg attcgctcag gctcttcgcg
     9721 gtgggcgaca tccagtacgc ggcgctgatc tgtttcgagg tcgcctttcc cgagtacgtg
     9781 cgcgccatgg tgcgccgggg ggcgaatttc ctggtcgaaa tcacgaatga cacctggttc
     9841 gagcgctcgg tgggagtgca catgcactcg cgggtgttta tcacccgcgc ggtggagaac
     9901 cgctcctggg gagcgcgcgc ggccaacagc gggctgacgt atatcgtgga cgactacggg
     9961 cggatccggg agggcctcga tctctacgcc gtgcaggtgc tggcggggaa agtcggcctc
    10021 ctgtacaatt actcggtgtt cacgagaatc ggcgatgtcg cggggcgggg gtcattcttg
    10081 cttacggcgg ggatcatctg tatattcatc gcactatggt gccggcaaaa actctcgcgg
    10141 ttttcctcgc gggcgcggtc ctgatcccgg cggcgctgcc gggcggggcg gccgcggccg
    10201 gggagtcctg gacgacgctg acgtcgttca agaccgtgcg ggcgttcgcg gttgtgggtg
    10261 acacgctcta cgccgccacg cccggagggc tgctgctgat cgccgactcg agttccccgg
    10321 gccgggccta cctgcacgcc gatggtctcg ggactaacga tctcgaggat gtgctggtcg
    10381 atgacgcggg gacagtgtgg ctggcgggtc tcgggcacct catccggttt gaccggcccg
    10441 cgccgcggca gttcctcttt gtcgaccgcg acgggcaggc gatccgtctg caggcgctgg
    10501 ccgacgacgg cgaccgcctg tgggtcggcg tgcacggggg gctggtgctg ttctccaaga
    10561 gcgacgatgg cgggcagatc gaagacagct acccgatgtt cggtactctc acgccctcgc
    10621 cggccgtggt cgatgtcgcg ctcgtgaacg acaccctctg gctggccacc tcggccggcc
    10681 tggcggcggc gcccgcggcc agtccggtgc tgctgaagac gcccgggacc tggacgacgt
    10741 ttgcggcggc ccggtacccg gaactggggc gggactcgat cgcgacggtg gaggcgttcg
    10801 gcggggagat ctatgcggcg acctctgccg gcgcgttccg gctcaatcgg gacggggcgg
    10861 atacgtcctt tgtcccggtc gcactcaccg ccggccgggc ggtcacagac ctggtggccg
    10921 aaggggactc gctgttcatc taccacagcg gcgggctggc agtggtgacg gccggcggcg
    10981 cggccgatct gccccgcgcc ggactcagcc tgccgccgac caccgggatc ggctgggagg
    11041 ggctgcgctg ggtgggtatg cgcgacagcg ggctgttcgt gggcgccgcc gggcagtacc
    11101 gggagtaccc ccacaccggg gcgccgggca acgacgtccg gacgattctg ttcggaccgg
    11161 agggacgcat caccgccggg ttcggcgaca agattttcgc gcagtatgac ggcgaccggt
    11221 ggatgcgccg gatcttctac cgccgtggcg acggcaccat ggatcttctg gcggacgggc
    11281 ggggcgatat ctgggccgca tcgtggggcg gcgggctgtt ccggatccgc ggcgattcca
    11341 tcacccagta caccgacagc aactccaccg tggtcggggt ggtggcgggc gatccgctcg
    11401 aatacacggt ggtgcggagc ctggccaccg acggcgtcaa tattttcgcc tcgctcttcc
    11461 aggcctacaa caaggacgtc gtggcctggg cgccgctcga ccgcgtgcac gacgaggcgg
    11521 cctggggagc gctgggcgtg cccgaagggc tggccaacga gtatgtcacg gcgctcgcgt
    11581 atgccgacgg cgagctggcg gtcgggacta tggcccgcgg ggtctacctc tgccggctgg
    11641 gcagcgcgcc ctaccggggg acggcgatca ccgtgcggca tttcaccgac gacaactccc
    11701 ggctcatctc caacgcgata cggacggtgg cctacgcgcc gaacggggag gcctggatcg
    11761 ggaccaacct gggaatctcg cgctgggact cgggcatcga gcggctggtc gatgtcgtgc
    11821 tgccggtcgg ggtggggacc gacgtggccg cgatcgtttt cgacgggcgg gggaacgcct
    11881 gggtgggaac gcgcggcgga ctcgtgcggc tcgacgccac cgggggggcg cccgacattt
    11941 tcaccgccgc caccagcgac ctcgtgtccg acgagatccg ggatctggcg ctggaccggc
    12001 gcagcggcga cctctacatt gcgacggcag gcggggtgtc ggtcctgcgg acgccgatcg
    12061 agaactttgt cgagacagtc ggggattcgg tggtgccgat ccccaatccg ttcgtgattc
    12121 gctccgccgc cgaccggctg cggttcaacc tcgaccgccc ggcgaccgtg agcatctaca
    12181 cggcggcggg ggaacgggtg gcggaatttc cggtcaatga accctgggac ggccgcaacg
    12241 accgcggccg cgcggtggcc tcgggcgcct acgtgtatgt catcaccgat gagacgggag
    12301 cctcgcaccg gggcaagatt ctgttggtgc ggaaggaatg aaagcgatct gctatcatga
    12361 tgacgacatg ccggaggagc cggtcagggc actgaccggc gattcctttc tggcatcggc
    12421 ggccttcgcc tcgctgtggg cggtccgcgg ggggcggcgc gccgtgtggg ccgcccaggc
    12481 gggggagggg atcgccgggg ttctccccgg catcgagttc gggcccgccc cgataaaacg
    12541 attcatggcc atgcccaacg ggctgtatgg gcgagtgttc gcggccgagg ggtccgaccc
    12601 gcgcaagacc ggagaagtgc tcctggaagc gatcgccgag gcggggtatg tcaaaaccta
    12661 cctgttcgac ttcttcggct gtttcacctc cggtacgggg ccgtttgcag tggccctgca
    12721 aacggcgctg ctggtcgaca tcggcgatcc cgcctgggaa ccgcccgacc ggaaactccg
    12781 gggcgaaatc cgcaaggccg agcgggaggg gctggcggtc ggacggatgg tctgggcgac
    12841 ccacggggag ggattcctga cgctgctgcg gggaaaagcg caccgacgcg gcggggaggc
    12901 ccgctacggt gcggatttct ggcaggccct cgcgcacgcc gcgcagcagg atgaacgcgt
    12961 ggcgtggtac tatgcgagct tcaacaaccg gccggtggcg tcgcacatct acttcgtcga
    13021 gggcgacatg ctcatcgggt ggcagatgta ctacgaccgg aagttctcgc cgctgaaggc
    13081 taatgcgtac ctgatcgccc gtgcctgcgc cgaggcgcgc gaccggggcg cgcggacgct
    13141 cgatctcggc gcgacgccgg agggggcggg cggaaccgag ttcttcaagc ggcgctgggg
    13201 cggacgggaa cacatctacc cgacgctcac gctgcggcgc ggaatcgggc ggtggctgtg
    13261 accggtgcgc acctgcgcgt gctcgtgctg gccgatgcgc ggtcggttca cacgcagcga
    13321 ttcgcggccg agttgcgccg gcaggggtgc gcggtgctgg tggcgtcgct ggaaggatcg
    13381 cccgttgacc ccgccctggg cggcgctgaa gaggatccga agcggaccgc tgctccggag
    13441 gccgcgggtg cgccgggaga accggaggag gggtgcggcg cctcggtccg gctgcggcgg
    13501 cggtttccca ttcggacggt cgcgtatgcc tgggcggcgc gtgaggtcga gcggctgatc
    13561 gagcggtggc ggccggaggc gatcagtgcg cactatgccg cggggtatgg ttacctgctc
    13621 agccgggtgc ggcggcggcc ccccgcgacg ctccacgtct gggggtccga tgtcctggtc
    13681 gtgccgcaca gatcgcggct gcacctgcgc aaggtggtgc gggggctgcg cgccgcagac
    13741 cacattttcg ccgactcccg ctaccttgcg gaagaggcgg ggcggctggc cggagtgacc
    13801 aacgtcacgg ttcacccgtg ggggatcgag cggcggcacc tgcagcggcg cctgcgcgtc
    13861 gggtcgatag ggcggccgct ccgcatcatc gtcccccgca tgcatgaaac cgtctacgat
    13921 aacgagtttg tcctcgaagc gctgcgggaa atgatcattc ggggggaggc gacggtgacg
    13981 tttccggcgt tcgggtcgag gtacgagcgg tttcggcggc gcgcggaggg gatggggttg
    14041 cgcgtcgttt ctctggaggc gggcgcggcg cgggacggca cgggaacgga agccgatcct
    14101 cgcggcgccg tctgctgcta cgagaagagg aaccgcgacg ggtttctcgc cctcatggcg
    14161 gcccatgatg tgtatgtgtc ggcggcgcga tccgattcct cgccggcgac gctgatcgag
    14221 gcgatggcgc tcggtctggc gccggtcgcg gccgatatcc cgggcgtgcg ggagtggctg
    14281 acgcccgaaa gcgggtttgc ctttcggccg ggggatgcgg agagcctccg gacgattctc
    14341 gcgcgcttgg cgaccgggga ggccggcggc gcggagtgcg cgctcgacgg cctgcggagg
    14401 cgcaaccggg agcaggtgga acgggaagcg atattcgagg acaacatcgc ggcacagctg
    14461 gcggtgatgc gcgctctgtg cgggaaagcg gggcactgat gcggcgggcg ctgctggtgt
    14521 gctactattt cccgccgctg agcggcgccg gcgtggcgcg gccgctggcc ctgttcaaac
    14581 atctgccgga gttcggctgg gagtgccacg tgctctcggt caagccggtg gcgtaccggg
    14641 cgttcgaacc ggaattgctg gacgggctgc ccggccggca catttaccgg gcgggatcgt
    14701 ccgatcccca gcggctcatg cgcctgatgg ggatacgaac ggtcgccgac cgggtgatcc
    14761 gcaagggacg ggcgctggcc gactggtatt tccccgaccc caagatcggg tgggtccgac
    14821 gggcgacggc gctgggacgg acactgctgg cgaaccggga ctatgacgcg atcatctcga
    14881 cctcgccgcc ggtctcggca cacctggttg cgcgcacgct cagccgggag ttcaagcggc
    14941 catggatcgc cgatttccgc gattattgga cgatccaccg gccggagcag ttgtacgcga
    15001 accggccgcg ccgccggcgg cgggcgcagc ggctcctggc ggcaattcgc gatgaagcaa
    15061 gcgaagtcac ggcggtgaac gagacggtga gggagtatgt cggggccggg accgtgatct
    15121 ccaactgcta cgatgaagcg ttggcggcgg ggtggactct gccggccgga agcccgttcg
    15181 tcatcggcgt cctcggcgca ctcaacgaga tcacgccggt ggccccgctc ctgcggctgc
    15241 tggcccgcct ccggagggag cggccggagg tgtttccccg cgtgagagtg cggcaggtcg
    15301 gagaggtcga cgggcagtgg ctcgagcgcc agatcgatgc ctgcggcgtg caggagattt
    15361 tcgatcgcta cggctggcaa ccccgacggg agacgatcgc gatcctctcc gaggcgtcgc
    15421 ttatgtacct cggggtggcc tcggcggagg agcgggggct gtcgaccggc cgcgtctaca
    15481 cgatgctggc ttcggggcgg ccgattctcg cttgggcgcc ggaagactcg gaactggcgc
    15541 gcctgctggc gcacaccggc agcggggtct gctttttcgg cagcgatgat tcgccagcgg
    15601 tgaagtacct ggccgatctg gtgcaggcgc acggggaggg gaagacacct gtgactcttc
    15661 gaccggccta tgccgaacct tactcctcgc ggcgaatgac ggagaagttc gcagcactgc
    15721 tggaccgtca gaaaccacag agctaccggt aagggatcgg atcgaccggt caggttcgat
    15781 gctgggctaa atgggggggc gcgcagagtt atccacatag ttatccacac aagtgaatag
    15841 ggtggcagga accgtcaggt cttatggttc cttcatcatc aattttcatt cggcgttatc
    15901 aattgccgtg atggaactta ccgcgatgtt ttttctccgg cggggggcac aaaaagtctt
    15961 gacttgaggc aataaaatgt gatatttgtg ggcgttcttg ggtcgaagtg gagaaaagtg
    16021 gaggaaacgc cttgtgaacg ggttcgtcgg ccggtataac acgactctcg atgagaaggg
    16081 tcgcttcgcg ctgccggcca aactccggag catcctgggt gcaggcggca agccgcagtt
    16141 ggacggcgcg ctgattctga cgaaagggct cgaggggtgt ttgagtctgt acaccgaacc
    16201 cgaatggaac gatatcgaga agcgtctttc ctcactgagt tttacccaaa gagattttcg
    16261 ttacttcggc cgccgctttt tctcgatggc ggcgtgggtg actcccgatc gcagcggccg
    16321 cattctcgtc ccgacgcatt taatcgccga ggccgatctc aagaaggagc tgctgctgat
    16381 cggtgtcaac aaatggattg aggtgtggaa tccggaacgc tacgaggatt acctccgggg
    16441 gtacgaggcc acctacgagg atgtagcgga gcggctgttc cctcgcccga gcggcgatga
    16501 ccggacctga gcccatccat gtcccggtgc tccgggacgc ggcagtgcgg ctctgggcgg
    16561 cgcggcggga cggcgcgtac ctggatctca cggttggcgc cgggggccac ctgaaagcac
    16621 tgagtgacta tctggacgga acggcccggc tgtacggcat cgaccgggac ccggcggcgg
    16681 cggcggccgc ggccgcccag cttgcgggag cgccgcagac ggtgacgatc gccagagggg
    16741 cttacggaga attcgaaacg gtgacggccg cgttcggcga ccggacttac gacggcatgc
    16801 tgatggatct cggtctttcg agcctgcagt tggacgatcc ccggcgcggt ttcacgtttc
    16861 gccatgacgg tccgctggat atgcgttttg atccggagtc gggcggtcct acggctgcgg
    16921 atctggtgaa ttcgctggga gaaaaggagc tggtcgatct gctgcacggg tacggcgagg
    16981 agcgccgggc caagcgggtg gcgcaggcca tagtcaggga gagacggatg cggatggtgc
    17041 agacaacatc gcagctggcc ggcctcgtcg agcgcatcgc cccggcccgg gatctccaca
    17101 aaacgctcgc gcgggtgttt caggcattcc gcattgccgt caacggcgaa ctggagcagt
    17161 tggccgcgac catgccgcgc ctgctgccgc gactgcgggc gggcgggcgg ctggtcgtga
    17221 tctcctacca ctcgctcgag gaccgtctgg tcaaaaggtt cttccaggcc gaggcgaaag
    17281 ggtgcgtgtg tccgccggag gcgccgctgt gcgcgtgcgg gcgcgctccg cggctgagga
    17341 ttctcacgcg ccgcgccgtc gtgccggacg agacggaggc ggcggcgaac ccgcgggcgc
    17401 gctcggccaa gctgcgggcc gcggaacggg tgggcgcgtg aaaaagaccg tgcgaaaatt
    17461 caaggagacg gtggagatcc ggtcctcgct gctcgtgcgt gtccgctccc accgctactt
    17521 cccgatcggc gcggtggccg gcgtgtttct gctcgccgcg tgcgtccata tctggcagcg
    17581 cgtgcacgtc atccacctcg cccaggagat cgcagtcctc cgcatggaga accggctgct
    17641 cggcgacgac acgcggaaag tgcacgcgga cctgtcggcg ctctcgatgt catcgcgggt
    17701 ggagacctac gcggccgatt cgctcgggct ggcgcaggtg gccgcgaccg agctgatcac
    17761 gctggtgccg gagggcgttc aggcgccgtc ggtcaaagac gacctccaca tggtggtgaa
    17821 aatggcggag cgctgggcga acgtcctgcc gagcgtgacc caggcgcagg cgaacgccgg
    17881 cgaactgatc agcccggtca tcgactccct ggccaacgga ggggacgact gatgcatcgg
    17941 acgcgcaccg aacgaatccg actcggcatc ctcttctcgc tcgtctgcct gtatttcctg
    18001 gcggtcgcgg cgcgcctggt ccacctccag gtggtcctcc accagaagta cgccgacatc
    18061 gtgcgcatcc agtcggaggg acgggtgaaa ataccggccg agcggggcct catttacgac
    18121 cggtacgggc gcctggtcgt gaacaacgtc aaatgcccgt cgctctatgc ctaccccggg
    18181 tcgcaaacgg agctggagaa cgccgcccgc tatgtcgaga aaatgttcgg cctgaagcgg
    18241 ggaacagcgc gcagagagta caacctcgag gtgaacaagt tcagctggat caagcgcctg
    18301 ctcggcgacg agttcgcccg gcacatcgaa aaaacggcgc cgcccgggct gtacctgcgc
    18361 gatgagacgc agcgggtgta tccctacgga ctggtcggca agcagattct gggtttcacg
    18421 aatatcgaca accaggggca gtccgggctg gagctggcgt gggacagcct catgaagggg
    18481 gaggacgggg aggcggatat ccggcgggac ggtctgcgca acgtcttccg cgtcaaagag
    18541 caggcggccg tgaaacccaa accggggcaa gcgctcgtcc tcactatcga tttcaacctc
    18601 caggaaatct tcgaacggca gctccgcaag ggggtcgagg agtacggggc gaaggcgggg
    18661 atgggggcgt tcatcgactg ccgcaccggt gagattctgg ccatggccca ctacgatccg
    18721 aaagaagagc acccggagaa gccggtcaag ctgcgggcga tcagcgacca gttcgaaccg
    18781 ggttcgtcgt tcaaagcgat cacggcggcg gccctgctgg aggcggggga ggtggacttc
    18841 cgcgagtaca cgtactgcga aatgggggcg tggaaaatgg aacggggcgg gacgctcaag
    18901 gacgacaaaa aacacggcaa cctcacgttc cgcgagatca tggagctctc gagcaacatc
    18961 ggagtggcca aatacggaat ccgcctgggc ggcgagaatc tctacgaggc ggcgcggaag
    19021 ttcgggttcg gacagaaata cggcatcggg ctcggggcgg aagagagcgg gcgtgtttcc
    19081 aagccccggc gctgggcgga ccgggccgtg gcctcgctgg ccatggggca cgcggtggcg
    19141 gtgaactgcc tgcaaatggc caatgccttc gccgccatcg ccaacggcgg cgaactcctc
    19201 cgcccccacc tgctcatgtg ctgcgtcgac gaacaccaga aagtgacccg ccggttcgga
    19261 cgggaagtaa tgcagaaggt gatggaggga gtctgggccg ataccctgaa agcggtcctg
    19321 cgcggcgttg tagagcgggg gacagcgacg aaggtgaatt cgaaagctgt cgccatcgcc
    19381 ggcaagacgg gtaccgccca gattcccgac tctgtcaccc accgctacaa agacgaatac
    19441 acggccagct tcgcgggctt cttcccgtac gagaagccgg ccatcgccgg gatcgtcatg
    19501 ctcatcgagc cgaacccgat ccactacggc ggctacaccg cggggccgat attccggcgg
    19561 acggccgagc agtacatgat cctcaacccc gacctgttcg actccggcaa ccagcttttg
    19621 gccgagcggt cgagcagccg cgaaggcacg gtcgaggccc ccaacctggt cggccggagc
    19681 ctggcggatg cggccgaaga cgcggaagag cgcgggttga cgatccgggc caaccggacg
    19741 gaggggatga tcgtgtggca gtatccgccg gccgaccggc tcatgttcgc cgaggatgac
    19801 ctgatcgtgg tagtggaatc gccggtcgag accgggttcg taatggcgga tttgaccggg
    19861 ctgtcgatca gggaagcatc ggcgtttctg gacttcgccg gtataaagtg cagaattaca
    19921 gggcgcggca aagttatctc acaatcgatc ggtcccggcc aggcggccgg gtcggaagac
    19981 gtatgtcagc tggaatgtcg gccgacatag gaggcagcca ttactctgcg tgagctggtg
    20041 cacgggtgcg ccggggcggt gatcaccggc gatcccgagg ttgcgatcga gcatctcgag
    20101 tacgactcgc ggctcgtgcg gcccggcacc ctgttctttg cggtgaccgg gttcacgcgc
    20161 gacgggtacg actttgtcgc cgacgttctt tcgcgggggg cggtggcggt catgggcgag
    20221 cgcgaatccc atccggcggt cccggtgcac gtccgcgtgc ccgatatccg ccaggccatg
    20281 gccgacgcgg cggcgaagtt ttacggcttt cccggcaaac agctcttcgc catcgccgta
    20341 accggcacca acgggaagac gaccacctgc tacctgctcc ggtgcatgct cgaggcggcc
    20401 gggcggaagg cgggactggt gagttcgacg ctctacgacg tgggcgggga gcagttcccg
    20461 gccgaacgca cgaccccgga ggcgctggac ctgcagcggc tgctgtttct catgcggaag
    20521 aacgggtgcg cggcggcggt catggaggtg tcttcgcacg ccctcgcgct caagcgggtc
    20581 gagcacattc tcttccgggt gggcgtgttc accaacatca cgcgtgacca cctcgatttc
    20641 cacaaatcga tggcggagta cctggcggcc aagaagcggc tgctgggcaa gctcgccggc
    20701 ccccacgcct gcgccgtgct caacctcgat gtgccggagt tccgcgcctt tttcggcgat
    20761 gcgcggtcgg agcatatgag ctactcgctc caggacacgc acgcggacgt ctacgcgagc
    20821 cgctacgaga tcgagccgat gcggacggtg ttcgacctgg ctacgccggc ggggatgcgg
    20881 acggtgacgt tcccgctgcc gggacggttc aacctgatga acgcggtggc ggcggcggcc
    20941 ggcgggctcg cggccggaat cgacctggac acggtggtgg ccgggctgga gagcgcgacc
    21001 ccggtgccgg ggcggttcaa cgcggtggat gccgggcagc ccttcgg
//
LOCUS       DAOPOE010000055        20797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3158_length_20797_cov_5.096567, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000055 DAOPOE010000000
VERSION     DAOPOE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3158_length_20797_cov_5.096567"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..983)
                     /locus_tag="PKM94_08520"
     CDS             complement(159..983)
                     /locus_tag="PKM94_08520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOD66719.1"
                     /translation="MRQYQQYDGPARGMSFGPGGVSPWIKWMFIVNGGMYVLQLIFPV
                     ITSYLGLTPARFFREFPIWLHTPLTYMFLHGGFLHVAFNMFALWMFGTEIEQTWGTGA
                     FRRFYLLAGLSGGAATLALNSGQAAVTVGASAAIYGVLVAYWFMFPNRLLYIYFLVPV
                     KVKYAIPGMIVLVFLAGGPSIAHMAHLGGALFAVVYMKTDWRWLRFGRSIKNLRHRRQ
                     EAKLQKNRERAEDIMKRVDAILDKINEVGIDNLTPEERRFLEEASSQLSGKPGEDK"
     gene            complement(1407..1733)
                     /gene="trxA"
                     /locus_tag="PKM94_08525"
     CDS             complement(1407..1733)
                     /gene="trxA"
                     /locus_tag="PKM94_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD66720.1"
                     /translation="MSKPITVTDDSFEQEVIQADKPVIVDFWATWCGPCKMIAPILEE
                     VAREYADRVKVAKIDVDSNTEVARKFGIMSIPSLIFFKNGEELDRVVGAIPKSQLTQR
                     LEKVLA"
     gene            complement(1875..6098)
                     /locus_tag="PKM94_08530"
     CDS             complement(1875..6098)
                     /locus_tag="PKM94_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOD66721.1"
                     /translation="MFTPTVLGIDIGSVTVKTVRLDDGGAVRKRYERFRGGPFETLQR
                     ILREDHADLVGRPVALGLTGIGGKTAQGILGGKFFGEIASIAAGNFHVCPDVRTAIEM
                     GGEDSKLLVLDGRRRTVSDFAMNAQCAAGTGSFLDQQAGRLKISIEDEFGRLALKSKK
                     PPRIAGRCSVFAKSDMIHLQQIATPDYDIVAGLCFAVARNFKSSIARGKRFDRPIAFE
                     GGVAHNPGMIRAFTEILGLAPGELIVPEHFEVIGALGAALLAAEDGDALCEFPPDRIA
                     SYHRFRAVAESGRPALAFRAPESKHYGVTLADRPSDIEGREVFLGIDVGSLSTNVVLI
                     DADRRVIARRYLMTEGRPIEAVRRGIEEIGAEVGGRVRVKAVGTTGSGRYLTGDFVGA
                     DVVRNEITAQATAAVNIDPTVDTIFEIGGQDSKYISIDDRAVVDFEMNKACAAGTGSF
                     LQEQAEKLDINIEREFGERALGAKCPVGCGERCTVFMESDLVAHQRTGAGKDDLLAGL
                     AYSIAANYLTKVVGDRRIGDRIFFQGGVAWNKAVVAAFENLTGKTVTVPPHHDVTGAI
                     GAAILAMEKVAEPGFRTRFKGFDLHKRRYDLSSFACEDCSNQCEIHKVEIEGEEPLFY
                     GARCERYDVSRRDDRPLPPDYLTWRQRLLFRRYVEVPAPARWRGRVGIPMVLHFYEYY
                     PFWKAFFESAGFQVVRSGMSSGAIVDDALELFTAETCFPIKLVYGHVNALLQRKVDYI
                     FLPSLIKVADQDEDDAEGAYICPYVQSIGSQLEARFDFAAEGVKFVSAQISLSHSVAV
                     LQQKLKGLVREFGLSEREFNRSVAAGLKAYRSYRQGVREAGERALRELGADERMLVVV
                     SRPYNGFDRRLSLDLPDKLRNLGYRAIPIDFLPAEQADSEFPYMYWRYGRRILQAADF
                     IRKHPGLFAVYITSFGCGPDSFITHFFRKQMAGKPYLQLELDEHSADAGLVTRCEAFV
                     DSLRFYRYTPPVGSFSVGRDEFRPFEKTIYIPNMCDHAYALRAAFARYGLTAEVLDEP
                     DELTLEYGKKFTSGKECFPCVVTTGEFIRRVRRPDFDRKRSVFFMPGADGPCRFGLYS
                     PFHRMVLDDLGFTDVPIYSPNSKDGYEDGFGLQGTDFRKTAWRGLVFVDGLQKMLLRT
                     RPYERTPGEAEKVYYHFLARMDRALVHDESLTELGREAARAFRQLRAEGPDRPLVGLV
                     GEIYLRHNRFSNNHLIGKLEQQGIEVRLATFTEWPLYTSLTFRRDSWLERDWRNTLKG
                     TLQVISQTRMEHRILAAMSEEFDLRHDYPVERVMKLASRYLSIDFKGEAILSIGKAIE
                     MVQEGAAGIVNAMPFNCMPGTIVSSLSRRVAEDLGQVPWLNISYEGLRDSGEETRLEA
                     FAEQVRTFARASGGAAERAPIESRPNGSA"
     gene            6220..6672
                     /gene="dtd"
                     /locus_tag="PKM94_08535"
     CDS             6220..6672
                     /gene="dtd"
                     /locus_tag="PKM94_08535"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013353029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOD66722.1"
                     /translation="MRVVLQRTTGCEVRIGGRLHAAAGPGLLLLFGTVSGDAEASCAW
                     LADKIVNLRIFEDEAGKMNRSALEVGAEMMVVSQFTLYADTRRGRRPSFDGAMAPDRA
                     EQLYDRFVELLRDTGLTVATGVFGAHMDVSLTNHGPVTFILEHGVPAA"
     gene            6653..7267
                     /locus_tag="PKM94_08540"
     CDS             6653..7267
                     /locus_tag="PKM94_08540"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOD66723.1"
                     /translation="MAYPQLERLARRRRLVLGSRSPRRLTLLRGMGVAFDQLIPEVEE
                     RRLPGEPPLAYACRLAEDKARWLMPRLAPDQVGLTCDTIVILGDDVLEKPSDSAEALA
                     ILSALSGTRHVVSTAIAWTADRRLLASGYETTEVYFRPVTAAQLRDYIATGEPMDKAG
                     AYGIQGMGAFLVDRIDGNLDTVIGLPRTLVEQLAGEAISQLEHL"
     gene            7278..8201
                     /locus_tag="PKM94_08545"
     CDS             7278..8201
                     /locus_tag="PKM94_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOD66724.1"
                     /translation="MGELETKFGELLKLERERQGMRLEDLAASLRISEANLRALEAGN
                     PAALPSPVYFNLFAKTYAESLGIDYAATMDAIKADMTEIEVPEPRKAKKGKAQPAPQA
                     APPPPPSAAESFAESAAAEPAAAGRKGRTLVVIGSVAAALVILAGGYFAVTQLLRADS
                     TQPVDASEEARLQTAYRNYNWTVPPYRPSDSLRLRLNSKGDAWTTVFADGDTSVVRNL
                     RPQEDVLISARHRLLVSIAVPSKVTVELNGRPVDLASPETGRISLVEITQANLDSIFT
                     AARPDTADTLAPADSGGAPAAPAPPAGGATP"
     gene            8203..8820
                     /locus_tag="PKM94_08550"
     CDS             8203..8820
                     /locus_tag="PKM94_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66725.1"
                     /translation="MLAGIKRFTARSQLSRLRKQMTPGTFQYPEALRTVRKILVCLPA
                     ELRELTTVKQFLPEIRDLFKTADITLLTAPGLQIADIFPRKGFNILTPSADQLNWSGL
                     ARKSYLKFLQDYRFDVVIDFGLEATDFTSSVLLSLPQAVRIGRGNHLGCPYYNLQIKT
                     RYLRDERNIYRSLLDTLRLLIDGRPKDEPAGPPPDDRRHGVSQET"
     gene            8801..12541
                     /gene="smc"
                     /locus_tag="PKM94_08555"
     CDS             8801..12541
                     /gene="smc"
                     /locus_tag="PKM94_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOD66726.1"
                     /translation="MYLKKLDILGFKSFANKTTVHFSHGITAVVGPNGCGKTNILDSL
                     RWVLGEQKTSLLRGAKMEEVIFNGTAEVKPMGMAEVSLTVVNDRGILPTEYSEVQITR
                     RLFRSGESEYLLNRVPCRLRDIQELFYDTGVGAHSYSVIQQDMIDAVISDKAEERRFL
                     FEEAAGITKYKQRKRIALRKLEATENDLLRLKDIYAEVQTQVRSLKRQYRKAERYQQE
                     LDQVKAWELYLGARRVRAIEAEKRELRAVLDELADGMTRGQTTVDRLSADIESERRRQ
                     TELEHHLVEIGAAVYEVTERAHALEKEISVLTEKRHSARALVERNERELAAMRERAAV
                     LDRQQSEATALGERQRAEFEDTARQVADAENAQAEADRNLLAARASRETENRSLLELE
                     GKLSSGKAEEDTLREQEQELAALLEDFRGQMESHRGEAQTAADQLAAARQKLADVTAR
                     KASAEQALADLTVRLESLLDLSGELAEELSSLAASLEAGQARKHLLEDMILQFDGYKS
                     GVVAVLEEADRWEGVLGTVADRFVPQDGLEIAAEAALGDMAGYLICDRRETAERIIDH
                     LRAESRGRIGILVPDTGMLNPVTRRPDLSAYPQFIGWLDQFISTADNLRPLMEAVLAR
                     TAVFAAGQDPAPILERLPFGFQAVSTDGRVYAKNIIAGGSDDAFPLFRRKEKVAEQQR
                     EIDELQARQADTHRRKNEVTAQIAAARAESGSLARGIEDIAEEMEAARAAIAEIEFRQ
                     RTLQADLERMDRERRQAGDKLERIRHRQYSLGLDHSRLAGQKDSLVSTMTATDERLAD
                     LERAAVLAADRVSRLQVAAVEARSRIEQTESRRTHVAELLDEIARATAEKTEEIGRAE
                     QEISDSARRINELEAALRETFAERTRVTDQQAALRAEQAEIMDRLTGCEKQLKELRAE
                     RESLSDRRHEKEMRLNTLESEARAIGDRVREDYGVDVAAIEAPKPDPAVDDQDAVHIL
                     AEQKERLKEFGAVNLLALEEYKTAAEREKFLGEQLTDLTTARNDLQTTINKINQTARK
                     LFHDTFEQVRGNFRKLFVELFSGGEADIRMIDPDDPLESDIDITARPRGKKLLSITMM
                     SGGERALTAISLLFSLYMVKPSPFCILDEIDAPLDDANCRRFLRIIDTFSAQTQFIII
                     THNKITMAAADNLYGVTMGQPGISQLVAVRFSSDDAGTTTVDTERPIEIGARAGAGEP
                     EPPRPRPSTKDREPEPEFELPQVIRDRLQQPAAPDAESDNAS"
     gene            12550..13488
                     /gene="ftsY"
                     /locus_tag="PKM94_08560"
     CDS             12550..13488
                     /gene="ftsY"
                     /locus_tag="PKM94_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOD66727.1"
                     /translation="MALFNPLDKLKNSLARTKDKLLGRLSEVVRRRAIDDDLLEDIEE
                     ILVQADVGLPATERLIARVKEAARERRLTEGDQIIDLLKEEIVAILSRTGRPLFPPGE
                     PRPAVWLIVGVNGVGKTTTIGKLATLFSREGKKVSIAACDTFRAAAIDQISIWAERSR
                     VALIRPQEGSDPAAVAFDAARAAHARGLDLLLVDTAGRLHTKSNLMEELKKIRRVIAK
                     AAPEAAISTRLILDGSTGQNALSQVRVFTDAVGCDGLIVTKLDGTAKGGVMIAVAQEL
                     NVPVDFIGLGEGIDDIQRFNPREYTEALFADEPAKR"
     gene            13508..13867
                     /locus_tag="PKM94_08565"
     CDS             13508..13867
                     /locus_tag="PKM94_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD66728.1"
                     /translation="MIDVTAQVRRAVRESGVASGIAVCFVPHTTAAVTINENADPDVA
                     RDILYKLDKEIPAADGYRHAEGNSDAHVKSTLVGVSAHVLIEGGDLVLGAWQGIYFCE
                     FDGPRRRHLIVRVMSGA"
     gene            13860..14330
                     /locus_tag="PKM94_08570"
     CDS             13860..14330
                     /locus_tag="PKM94_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOD66729.1"
                     /translation="MLNITVLAVGRDKDRWVSEAVAHYEKLLARWCRLAWRIVPSPKH
                     SAALSPEEIRRAESARLRAALGPGYIAALAASGRTVDSPAFARLLERWHTVSGSNLTF
                     VIGGPHGLDRDLLSRADTVLSLSPLTFSHQLVRLILLEQLYRAFSILHGTDYHK"
     gene            complement(14383..14961)
                     /gene="pyrE"
                     /locus_tag="PKM94_08575"
     CDS             complement(14383..14961)
                     /gene="pyrE"
                     /locus_tag="PKM94_08575"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOD66730.1"
                     /translation="MSQDDVVNLFKESGALLTGHFKLTSGRHSDVYYEKFTLLKQPAL
                     CTRLCARMADTLRTTGVRTIVGPTTGGIILAYDVARYLGVEAIYAEAADDGKGRVFKQ
                     GFHLEPGQKVAIVDDVLTTGTSVDEVIALVRRYGAEIVGLRLMLDRSNGKVRFDYPFA
                     ALATVAAESWDPAECPLCAKAMPLTQRGSRKF"
     gene            complement(14958..15332)
                     /gene="acpS"
                     /locus_tag="PKM94_08580"
     CDS             complement(14958..15332)
                     /gene="acpS"
                     /locus_tag="PKM94_08580"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOD66731.1"
                     /translation="MIQAIGLDIVEVARMERDLRTWGNRFAERILGAEELAILNRRHD
                     KSVFLAGRFAAKEAVIKALGYRLSERPAYRAIAITNDASGQPVLSLPDDIRAALGRAR
                     CFLSITHERTHAAAVAVITEDS"
     gene            15803..17614
                     /locus_tag="PKM94_08585"
     CDS             15803..17614
                     /locus_tag="PKM94_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOD66732.1"
                     /translation="MKLPSLAQLAAEYGRTIRRFPLVLVCALLGTLAALILVDQEGSR
                     EASVLYNILLTAILALPVMTAVKLAAERSSAPASSAWPAQAAAAALLVVYAATVPTDL
                     PRAPLVHLLRFFALGIGCTLLASVWPFRQKGEDNRFWHFTSLVVFRIMLAGAFALVLF
                     AGLALALAALDNLFGMAIPSKRYAELWILTLGLFAVPYVLAGLPEDWSALGTGSDYPR
                     PLKVLGQYVLPPLVAVYFVILYAYIAKIIVTWSWPQGWVGRLIIGFSATGILALSVLD
                     PMRERTESLWIKRASRWFYLILLPLIVVLFLALWRRISEYGLTEGRYLGLAVGVWLAV
                     IAAYFLLSRSKSIRMIPASLCAFAFLISVGPWGMFSVSEQSQIRRLRGMLTADSILVN
                     GAVTKAPAPVPADHEVQISAILIYLYEVHGFSALEPWFGETLRADTEDAGNRFKGPEY
                     VAELLGIEFHPYDTPGREQYFAVRIDPQTPISVAGYDHMLRAGYGGRAAAPSPAGEPV
                     AGAIETAADSVVVRFVLDSTTTDSVTVSLRPLIDRIVAARADARGSEISGEEAMIENE
                     TGGVKVKVVMLQADIRRDDSITVLNSYDALILYSRRK"
     gene            complement(17737..18294)
                     /gene="frr"
                     /locus_tag="PKM94_08590"
     CDS             complement(17737..18294)
                     /gene="frr"
                     /locus_tag="PKM94_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOD66733.1"
                     /translation="MVDKIEKDAHDRMHKTIETLKREFGTVRTGKASPHLLDTVTVEA
                     YGTQMPLNQVATVTAPEPRLLVVTPFDKSTVGNVVKGIQAADLGFNPMVDGQIIRVPI
                     PALNEERRRTLVKQCKHLAEEARVAVRNIRRDANEHLKQAEKDSKISEDEMRKSLDKV
                     QKLTDEHIKEIDSLLTVKEKEVMEV"
     gene            complement(18326..19054)
                     /gene="pyrH"
                     /locus_tag="PKM94_08595"
     CDS             complement(18326..19054)
                     /gene="pyrH"
                     /locus_tag="PKM94_08595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOD66734.1"
                     /translation="MDTDAPLYKRVLIKISGEALMGAREFGIDSPTVEAVCGQIIEVK
                     QLRCEIGIVVGGGNIFRGLAATELGMDRVTADNMGMLATVINALAMMDTLEHMGVYTR
                     VMSAVKLEAFAEPFIRRRAVRHMEKGRVVIFAAGTGSPYFSTDTAASLRAMEVGADLM
                     IKATNVDGVYSADPRKDAKATFYPRLSYMDILTRELRVIDATAISLLKENQIPLRIVD
                     LHRPGNLRRTVLGEDVGTLVTSAG"
     gene            complement(19058..19651)
                     /gene="tsf"
                     /locus_tag="PKM94_08600"
     CDS             complement(19058..19651)
                     /gene="tsf"
                     /locus_tag="PKM94_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOD66735.1"
                     /translation="MDISAQLVKELREKTGAGMMDCKKALTETHGDLSKAIDYLREQG
                     ISKAAKKEGRATAEGVIATYVHPGDKLGVMVEVNCETDFVARTDQLKEFARNIAMHIA
                     ASAPLVVSRDEVDPALIAKEREIYRHQTLKEGKPEKIVDKIVDGRIEKYFGEVVLMEQ
                     PFVKDADKTIEEYLKETIARLGENMKVRRFARFRLGE"
     gene            complement(19665..20453)
                     /gene="rpsB"
                     /locus_tag="PKM94_08605"
     CDS             complement(19665..20453)
                     /gene="rpsB"
                     /locus_tag="PKM94_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOD66736.1"
                     /translation="MVSPQVKDLLEAGVHFGHQTRRWNPKMKPFIFAARNGIYIIDLQ
                     KTVNALEQARRKMAETVGKGKSVLFIGTKKQAQDVIKEESARCGGYYVTERWLGGMLT
                     NFQTIKHSVKKLKDIEKMRVDGTLEKFSKKERSRIEHEAGKLQKYLGGIKEMGHLPGL
                     VVIVDARKERIAVAEAHKLGIPIIGIVDTNADPDLIDFPIAGNDDAIKSIRILMHALV
                     DAAVEAKSTVVTEMLAASEREGAPGNGHSPAAGRHDVANKDQAE"
     gene            complement(20596..>20797)
                     /gene="rpsI"
                     /locus_tag="PKM94_08610"
     CDS             complement(20596..>20797)
                     /gene="rpsI"
                     /locus_tag="PKM94_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD66737.1"
                     /translation="CRASGGGLSGQAGAVRLAVARALARLNEDFRPTLRQNGLLTRDP
                     REVERKKYGRPKARKRFQYSKR"
BASE COUNT         3514 a         7159 c         6412 g         3712 t
ORIGIN      
        1 gagcacgagg tccggttttc cggtctggag cacctgcacg gccgcgtcgc tctcggtgac
       61 cgccaccacg tcgtagccgt gctggcgcag gatcgtctcc gccacactcc gggtcgcatc
      121 ggaccgttcc gccagcaata ctttttttcg cattctcgct acttgtcttc cccgggcttg
      181 cccgatagct gcgatgacgc ctcttcgagg aacctccgtt cctccggcgt gaggttgtcg
      241 atgccgacct cgttgatttt gtcgaggatg gcgtcgaccc gtttcattat gtcctcggcc
      301 ctttccctgt ttttttgaag cttggcctcc tggcggcgat gacgcaagtt tttaattgag
      361 cgcccgaagc gcagccaccg ccagtcggtc ttcatgtaga cgaccgcgaa cagcgcaccg
      421 cccaggtgcg ccatgtgcgc gatgctcggt cccccggcga gaaacacgag cacgatcatc
      481 ccgggaatgg cgtacttgac cttgaccggc accagaaagt agatatacag cagccggttg
      541 gggaacatga accagtaggc cacgagcacg ccgtagatcg cggccgaggc gcccacagtc
      601 accgccgcct gtccggagtt cagcgccagc gtggccgccc cgcccgacaa cccggccagc
      661 aggtagaagc gccggaacgc gccggtcccc cacgtctgct cgatttcggt cccgaacatc
      721 cacagcgcga acatgttgaa agcgacgtgg aggaacccgc cgtggaggaa catgtaggtc
      781 agcggggtat gcagccagat cggaaactcg cggaagaacc gggccggggt gagcccgagg
      841 taggaggtga tcaccgggaa gatgagttgg agaacgtaca tccccccatt gacaataaac
      901 atccacttga tccaggggga gactccgccc ggaccaaaac tcatcccgcg cgccggcccg
      961 tcgtattgct gatactgcct catccgtctt agatatcggt tgccgtgccg gatccgatca
     1021 tgagcgcatc cgtcaatttc tgtcgggcgc ttctttccgt accgcctcgt cggttgtatc
     1081 ctgttgatcc cgtttatctt ccaccggaag atcacagcgc ccgggcagtc agcccgcgcc
     1141 gaatttgggc aggactcgcg tgatcagcac cagccagaca accagaaaaa ccgccagaac
     1201 tgcgatgtcg taccaactca tatactagcg ctgtcctccg ggaaaaatca agtccattgt
     1261 ccgcgctctc ggcacgggcc gtctgcttct gtaatcggcg gcccccgcca aaagttccgc
     1321 gcggggccgc cggaggggtg ggcgcaaaaa cgccgacggc ttcagtggcc gccggcgcct
     1381 gtgcctgcac tgccgagaat cgaattctag gcgagaactt tctccaaccg ctgcgtcaac
     1441 tgcgacttcg gaatcgcccc caccacccga tccagttcct cgccgttctt aaagaagatc
     1501 agcgagggaa tcgacatgat cccgaacttg cgcgccactt cggtattcga atccacgtcg
     1561 atcttggcga ccttcacccg gtcggcatac tcccgggcga cttcctcgag gatgggcgcg
     1621 atcattttgc acggtccgca ccaggtcgcc cagaagtcga cgatcaccgg cttgtcggcc
     1681 tgaatcactt cctgctcgaa tgaatcatcg gtcaccgtaa tcggcttgct catgactgta
     1741 gtcctccgta tctgtcaggc gtcaccacgt tgtctgctgt aacacatctt cgcgccggcg
     1801 gttccttcgg cgcttccggc gcaagacaca atgatatcgc ccgtgtctcg gcccgccaag
     1861 cgaaatccgc cggcctaagc ggagccgttc ggtcggcttt cgatcggcgc tcgctcggcc
     1921 gcgcccccgc ttgcccgcgc gaacgtccgc acctgttcgg caaacgcctc gaggcgcgtc
     1981 tcctcccccg agtcgcgaag tccctcgtag ctgatattca gccaggggac ctgcccgaga
     2041 tcctcggcga cccggcgcga cagcgacgac acgatggtgc cgggcatgca gttgaacggc
     2101 atggcgttca cgatcccggc cgccccttcc tgcaccatct cgatcgcctt gccgatcgag
     2161 aggatcgcct cccccttgaa atcgatcgac aggtagcggc ttgccagttt catcacgcgc
     2221 tcgaccgggt agtcgtgccg caggtcaaac tcttcgctca tggccgccag aattctgtgc
     2281 tccatccgcg tctgcgatat cacctggagc gttcccttga gcgtgttcct ccagtcgcgc
     2341 tccagccacg agtcgcggcg gaacgtcagg gaggtgtaga ggggccattc ggtgaaagta
     2401 gccagccgca cctcaatccc ctgctgttcg agttttccga tgaggtggtt gttcgaaaag
     2461 cgattgtgcc ggagatagat ctcgccgacc aggcccacga gcggccggtc cggtccctcc
     2521 gcccgcaatt gtcggaacgc ccgcgccgcc tcccgtccga gctccgtgag cgattcgtcg
     2581 tgcaccagcg cgcgatccat gcgcgcgaga aaatggtagt acaccttctc cgcctccccc
     2641 ggcgtgcgct cgtacggccg cgtccgcagc agcattttct ggaggccgtc cacgaacacc
     2701 agcccccgcc aggcggtctt ccggaaatcc gtcccctgca gcccgaaccc gtcttcgtat
     2761 ccatccttcg aattgggcga atagatcggc acgtcggtga acccgagatc atcgagcacc
     2821 atgcggtgaa agggcgagta cagaccgaac cggcacggtc cgtcggcccc cggcatgaag
     2881 aaaaccgagc gcttgcggtc gaaatccggc cgccgcacgc gccggatgaa ctccccggtg
     2941 gtcacgacgc aggggaagca ctccttgccc gaggtgaact tcttgccgta ctcgagcgtc
     3001 aactcgtccg gctcgtcgag gacttcagcc gtcagcccgt agcgcgcgaa ggccgcccgg
     3061 agggcgtagg cgtggtcgca catgttcggg atatagatcg tcttttcgaa ggggcggaat
     3121 tcgtcgcgcc ccacactgaa actgccgacc ggtggcgtgt accggtagaa ccgaagggag
     3181 tcgacgaagg cctcgcaccg cgtcaccagt cctgcatccg ccgagtgctc gtccaattcc
     3241 agttgaaggt acggcttccc cgccatctgc ttgcggaaga agtgggtgat gaaggagtcc
     3301 ggtccgcacc cgaacgaggt gatgtagacg gcaaagagac cggggtgttt ccggatgaag
     3361 tcggccgcct ggaggatccg ccggccgtag cgccagtaca tataggggaa ctcggaatcc
     3421 gcctgctccg ccgggaggaa gtcgatcggg atcgcccggt agccgaggtt gcggagcttg
     3481 tccggcaggt ccagcgacag ccgccggtcg aacccgttgt aggggcggct gaccacgacc
     3541 agcatccgct cgtcggcgcc cagctcgcgc agggcccgct ctcccgcctc ccgcacgccc
     3601 tgccggtagg accggtaggc tttcaggccc gcggccaccg agcggttgaa ttcccgctcc
     3661 gagagcccga attcccggac cagtcctttg agtttctgct gcagcacggc caccgagtgg
     3721 ctcagcgaaa tctgcgcgct gacgaatttc accccttcgg cggcaaaatc aaaccgcgcc
     3781 tccagctgcg acccgatgct ctgcacgtag gggcagatgt aggccccctc ggcgtcgtcc
     3841 tcatcctggt ccgccacctt gatgagcgac ggcaggaaga tgtaatccac cttgcgctgc
     3901 agcagggcgt tcacgtgccc gtagacgagc ttgatcggga agcacgtttc ggcggtgaac
     3961 agctcgagcg cgtcgtccac gatcgccccg ctcgacatcc ccgaccgcac cacctggaat
     4021 ccggcggact cgaagaacgc cttccagaac gggtagtact cgtagaagtg gagcaccatc
     4081 ggaatcccca cccgtccgcg ccagcgggcg ggcgcgggga cctcgacata ccgtcggaac
     4141 aacagccgct ggcgccaggt caggtagtcc ggcggcaacg gccggtcgtc gcggcggctc
     4201 acgtcgtacc tctcgcaccg cgcaccgtag aagagcggct cctctccctc gatttccacc
     4261 ttgtggatct cgcactggtt ggagcagtcc tcgcaggcga aagaggagag gtcgtagcgc
     4321 cgtttgtgca gatcgaaccc tttgaagcgg gtgcggaagc ccggttcggc gactttctcc
     4381 atggccagga tcgccgcccc gatcgccccc gtgacgtcgt ggtgcggcgg cacggtgacg
     4441 gtcttgcccg tgaggttttc gaaagccgcc accacggcct tgttccaggc cactccgccc
     4501 tggaagaaga tgcggtctcc gattcggcgg tccccgacca ccttggtgag gtagttggcg
     4561 gcgatagagt aggccaggcc ggcgagcaga tcgtccttcc cggcccccgt ccgctggtgg
     4621 gccacgaggt cggactccat gaagaccgtg cagcgctccc cgcacccgac ggggcacttt
     4681 gcgcccagcg cccgctcgcc gaactcccgc tcgatgttga tgtcgagttt ctccgcctgc
     4741 tcctgcagga acgatccggt cccggccgcg cacgccttgt tcatctcgaa gtccacgacc
     4801 gcccggtcgt cgatcgagat atacttggaa tcctgtccgc cgatctcgaa gatggtgtcg
     4861 acggtcgggt cgatgttgac ggcggccgtc gcctgggccg tgatttcatt gcgcacgacg
     4921 tcggccccga caaagtcgcc ggtcaggtat ctgcccgaac cggttgttcc gaccgctttc
     4981 acccggaccc ggccgcccac ctcggcgccg atttcctcga ttccccggcg cacggcctcg
     5041 atcggccgcc cctcggtcat gaggtagcgc cgggcgatca cgcggcggtc ggcgtcgatc
     5101 aggaccacgt tggtcgagag cgacccgacg tcgattccga gaaacacctc gcgcccctcg
     5161 atgtcgctcg gccggtcggc cagcgtcaca ccgtagtgct tgctctccgg cgcgcgaaaa
     5221 gcgagggcgg gccgccccga ttccgcgacc gcccgaaacc ggtggtagga ggcgatccgg
     5281 tcgggcggga actcgcagag ggcgtcgccg tcctcggccg ccaggagggc cgcgccgagc
     5341 gcgccgatga cctcgaagtg ttccgggacg atcagttcgc ccggggcgag gccgaggatt
     5401 tcggtgaagg cccggatcat tccggggttg tgcgccaccc ccccctcgaa cgcgatcggc
     5461 cggtcgaacc gtttcccccg cgcgatcgaa ctcttgaaat tgcgcgccac ggcgaaacac
     5521 agcccggcca cgatgtcgta gtcgggcgtg gcgatctgct gcaggtggat catgtccgat
     5581 ttggcgaaga ccgagcaccg cccggcgatg cgcggcggct tcttcgactt cagcgccaac
     5641 cggccgaact catcctcgat cgaaatcttc agccgccccg cctgctggtc gagaaacgac
     5701 cccgtgcccg ccgcgcactg cgcattcatg gcgaaatcgc tcaccgtccg ccgccgtccg
     5761 tccagcacca gcagcttgga gtcttcgccc cccatctcga tggcggtccg cacgtccggg
     5821 cagacatgga aattgcccgc ggcgatcgag gcgatctcgc cgaagaattt cccgcccaga
     5881 atcccctgcg cggtcttgcc gccaatgccc gtcagcccca gcgccaccgg gcgtcccacc
     5941 aggtcggcgt ggtcttcccg aaggatgcgc tgcagggttt cgaacggtcc gccgcggaat
     6001 cgctcgtagc gcttgcggac cgccccccca tcgtccagtc gaacagtctt caccgtgacc
     6061 gagccgatgt cgatgccaag tactgtcgga gtgaacatgc cggcaaagaa cggcggcggc
     6121 gcgcacgggg caagtctttt tgcgcgggcc ggcgcgccgt ttcccgttgt ctttttgccg
     6181 ccgccgccta tattcaggga cttaagtgga gactgtccga tgagagttgt cctccagcgc
     6241 acgaccggct gcgaagtccg gatcggcggc cgccttcatg ccgccgcggg tccgggcctg
     6301 ctgctcttgt tcggcaccgt cagcggcgac gccgaggctt cgtgcgcctg gctcgcggac
     6361 aagatcgtca acctgcgcat ttttgaggac gaggccggca agatgaaccg ctcggcgctg
     6421 gaggtgggcg ccgagatgat ggtcgtttcg cagttcacgc tctacgccga tacccgccgc
     6481 ggccgccggc cctcgttcga cggcgcgatg gcgcccgacc gggcggagca gttgtacgac
     6541 cgtttcgtgg aacttctccg ggacaccggc ctcaccgtcg ccacgggcgt gttcggcgcc
     6601 cacatggacg tcagcctcac caaccatggc ccggttacat ttatcctcga acatggcgta
     6661 cccgcagctt gagcggctcg cgcgccgccg ccggctcgtc ctggggtcca ggtcgccgcg
     6721 gcgcctcacc ctgcttcgcg gcatgggcgt ggcattcgac cagttgatcc cggaggtcga
     6781 ggagcgccgc ctccccggcg agcctccgct tgcctacgcc tgccgactgg ccgaggacaa
     6841 ggcgcgctgg ctcatgcccc gcctggcccc ggatcaggtc gggctcacct gcgacacgat
     6901 cgtcattctg ggcgacgacg tcctggagaa accgtccgac agcgccgagg cgctggccat
     6961 tctctccgcc ctctcgggca cgcgccacgt ggtctccacg gcgatcgcct ggaccgccga
     7021 ccgccgattg ctcgcctccg gctacgagac caccgaggtg tatttccggc cggtcaccgc
     7081 cgcccagctt cgcgactaca tcgccaccgg cgagccgatg gacaaagcgg gggcctacgg
     7141 cattcaggga atgggggcgt ttttagttga cagaattgat gggaatctgg atactgtcat
     7201 aggtctccct cgaacgctcg tcgagcagct cgccggagaa gcgatcagtc agttggaaca
     7261 tctgtaagat actgcacatg ggtgagttag agacaaaatt tggagaattg ctcaagctgg
     7321 agcgggaacg ccaggggatg cggcttgagg atctcgccgc cagcctccgg atttccgagg
     7381 ccaacttgcg ggcgctggag gcgggcaacc cggccgccct tccctcgccg gtctatttca
     7441 atctgtttgc caagacctac gccgagtctc tcggtatcga ctatgcggcc accatggatg
     7501 ccatcaaagc cgacatgact gagatagagg ttccggaacc gcggaaggcg aagaaaggca
     7561 aggcgcagcc ggcgccgcaa gcggccccgc cgccccctcc gtcggccgcc gaatcgttcg
     7621 ccgagtcggc ggcggccgaa ccggccgccg cgggcaggaa gggccgcacg ctggtggtca
     7681 tcgggtcggt cgcggccgct ctcgtcatct tggcgggcgg atacttcgcg gtcacccagt
     7741 tgctccgcgc cgacagcacg cagccggtcg acgcaagcga ggaggcgcgt ctgcagaccg
     7801 cgtaccgcaa ctacaattgg accgttcctc cctaccggcc ctccgactcg ctgcgcctgc
     7861 ggctgaactc gaagggggat gcctggacga cggtctttgc cgacggcgac acgtccgtcg
     7921 tccgcaacct gaggccccag gaggacgtgc tcatatcggc gcgccaccgg ctgctggtgt
     7981 cgatcgccgt cccctccaaa gtgaccgtcg agctgaacgg ccgcccggtt gacctggcct
     8041 cgccggagac cgggcgcatc tcgctggtgg agattacaca ggccaatctg gattccattt
     8101 tcaccgctgc gcgccccgac accgccgata ctctcgcgcc cgccgattcc ggcggcgctc
     8161 cggcggcgcc ggccccgccc gcgggaggag cgacgccctg acatgttggc cggcataaag
     8221 agattcacag cgcgctccca gttgagccgc ctgcgcaagc agatgacgcc ggggacattc
     8281 cagtaccccg aggctctccg cacggtgcgc aaaatcctcg tctgtctccc cgccgagctc
     8341 cgcgagctga cgaccgtgaa gcagttcctt ccggaaatcc gtgacctgtt caagaccgcc
     8401 gacatcaccc tgctcaccgc ccccggcctt cagatcgccg acatcttccc ccgcaaaggg
     8461 ttcaacatcc tcaccccgtc ggccgaccag ctcaactgga gcgggctggc ccggaagagc
     8521 tatctgaagt ttctccagga ctaccgcttc gacgtcgtga tcgacttcgg tctggaggcg
     8581 accgatttca cctcgagcgt gctcctctcg ctgccccagg cggtgcgcat cgggcgcggc
     8641 aaccacctcg gctgccccta ctacaacctg caaatcaaga cgcggtacct gcgggatgag
     8701 cgcaacatct accggtctct tctcgacacg ctgcggctgt tgatcgacgg tcgtccgaaa
     8761 gacgaaccgg ccggaccgcc ccccgatgat cggaggcatg gtgtatctca agaaacttga
     8821 tatcctcggc ttcaaatcgt tcgccaacaa aactacagtc cacttttccc acggcatcac
     8881 ggccgtcgtc gggcccaacg gctgcggcaa gaccaacatt ctcgactcgc tgcgctgggt
     8941 ccttggcgaa cagaaaacct ccctcctccg aggcgccaag atggaggagg tcatattcaa
     9001 cggcacggcc gaggtcaagc cgatgggcat ggccgaggtc tcgctcacgg ttgtcaacga
     9061 ccgcggcatt ctcccgaccg agtattccga ggttcagatc acgcgccgcc tcttccgcag
     9121 cggggaatcg gaatacctcc tcaaccgcgt tccctgccgc ctccgggaca tccaggagct
     9181 cttctacgac accggcgtgg gggcccactc ctactcagtc atccagcagg acatgatcga
     9241 tgccgtcatt tccgacaaag ccgaggagcg ccgtttcctc ttcgaggagg cggccggcat
     9301 caccaagtac aaacagcgca agcgcatagc gctgcggaag ctcgaggcca ccgagaacga
     9361 cctcctgcgc ctcaaggaca tctacgccga ggtccagacg caggttcgct cgctcaagcg
     9421 ccagtaccgc aaggccgagc gctaccagca ggagctcgac caggtgaagg cctgggagtt
     9481 gtatctcggc gcccgccgcg tccgggcgat cgaggcggag aagcgcgagc tgcgcgccgt
     9541 cctcgatgaa ctcgccgacg gcatgacccg gggccagacg acggtcgacc gcctctcggc
     9601 cgacatcgaa tccgagcgcc ggcggcagac cgagctggag caccacctgg tggagatcgg
     9661 cgccgcggtc tacgaggtga ccgaacgcgc ccacgctctg gaaaaggaaa tctccgttct
     9721 gaccgagaag cgccacagcg cgcgcgccct cgtcgagcgc aacgagcgcg agctggcggc
     9781 catgcgcgag cgggccgcgg ttctcgaccg gcagcagtcc gaggccaccg ccctgggcga
     9841 gcggcagcgc gccgagttcg aggacaccgc ccggcaggtg gccgacgccg agaacgccca
     9901 ggcggaggcc gaccgcaacc tcctggccgc ccgcgcctcg cgcgaaaccg agaaccgcag
     9961 cctcctcgaa ctcgagggca agctctcctc gggcaaagcc gaggaagaca ccctccgcga
    10021 acaggagcag gaactggccg cgcttctgga ggacttccgc ggacaaatgg agagccatcg
    10081 cggcgaggcg cagacggcag ccgatcaact cgccgcggcc cggcagaagc tcgccgacgt
    10141 gacggcgcgc aaggcctcgg ccgagcaggc gctcgccgat ctcacggttc ggctcgaatc
    10201 cctccttgac ctcagcggcg aactggccga ggagctctcc tcgctggccg cctcgctcga
    10261 ggccggccag gcgcgcaagc atctccttga ggacatgatc ctgcaattcg acgggtacaa
    10321 gtcgggcgtg gtcgccgtgc tcgaggaggc ggaccgctgg gagggcgtcc tcggcacggt
    10381 cgccgaccgc ttcgtaccgc aggacggtct ggagatcgcg gccgaggcgg ccctcggcga
    10441 catggcgggg tacctgatct gcgaccgccg cgagacggcc gagcggatca tcgaccacct
    10501 ccgcgccgag agccggggcc gcatcggcat cctcgtgcct gataccggca tgctcaatcc
    10561 ggtcacgcgc cggcccgacc tctcggccta cccgcagttc atcggctggc tcgaccagtt
    10621 catctcgaca gccgacaacc tgcgcccgct catggaggcc gttctcgccc gcaccgcggt
    10681 attcgccgcc ggccaggacc cggcgcccat tctcgaacgc ctccccttcg gcttccaggc
    10741 ggtgtccacg gacggccggg tgtatgccaa gaacatcatc gccggcggct ccgatgacgc
    10801 ttttccgctg ttccggcgca aggagaaggt ggccgagcag cagcgggaga tcgacgagct
    10861 ccaggcccgg caggctgaca cccaccgccg caagaacgag gtgacggcgc agatcgccgc
    10921 cgcccgggcc gagtcgggat cgctcgcccg ggggatcgag gatatcgccg aagaaatgga
    10981 agcggcccgc gccgccatcg ccgaaatcga gttccgtcag cgcacgctgc aggccgatct
    11041 cgagcgcatg gaccgcgagc gccgccaggc cggcgacaag ctcgaacgca tccgccaccg
    11101 gcagtacagc ctcggcctcg atcacagccg cctggcgggt cagaaggaca gcctggtgag
    11161 cacgatgacg gctaccgacg agcgtctggc cgacctcgag cgtgccgccg tgctggccgc
    11221 cgatcgggtc tcccgtctgc aggtcgcggc ggtcgaggcc cgcagccgca tcgagcagac
    11281 cgaaagccgc cggacccacg tcgcggagtt gctcgatgag atcgcccgcg ccacggccga
    11341 gaagaccgag gagatcggcc gcgccgaaca ggagatttcc gacagcgccc ggcgcatcaa
    11401 tgagctcgag gccgcgctcc gcgagacatt cgcggaacgc acccgcgtca ccgatcagca
    11461 ggcggccctc cgcgccgagc aggccgagat tatggaccgg ctcaccggct gcgagaaaca
    11521 gctcaaggaa ctccgcgccg aacgggaatc cctctccgac cgccgccacg aaaaggagat
    11581 gcgcctgaac acgctggagt cggaggcccg cgccatcggc gaccgcgtcc gcgaggacta
    11641 cggagtcgac gtcgccgcga tcgaggcgcc caagcccgac ccggccgtcg acgatcagga
    11701 tgccgtccac atcctcgccg agcagaaaga gcgcctcaaa gagttcggcg cggtcaacct
    11761 cctggctctc gaagaataca agacggccgc cgagcgggag aaattcctcg gcgagcagct
    11821 caccgacctc acgaccgccc gcaacgacct gcagacgacg atcaacaaga tcaaccagac
    11881 ggcccgcaag ctcttccacg acaccttcga acaggtgcgc ggcaacttcc gcaagctctt
    11941 tgtcgagctc ttctcgggcg gcgaggccga catccgcatg atcgatcccg acgatccgct
    12001 ggagagcgac atcgacatca cggcccgccc gcggggcaag aaactccttt cgatcacgat
    12061 gatgtcgggc ggcgagcggg ccctcaccgc catctccctc ctcttctcgc tctacatggt
    12121 caaaccctcg ccgttctgca ttctcgatga gatcgacgcg ccgctcgacg atgccaactg
    12181 ccggcgcttc ctccgcatca tcgacacttt ctccgcgcag acgcagttca tcatcatcac
    12241 ccacaacaag atcacgatgg cggcggcgga caatctctac ggcgtgacca tgggacaacc
    12301 cggcatctcg cagctcgtcg ccgttcgctt cagttccgac gacgcgggca ctacgacggt
    12361 cgacaccgag cggccgatcg agatcggggc gcgcgcaggc gccggggaac cggaaccgcc
    12421 gcgcccgcgc ccgtccacaa aggaccggga gccggaaccg gaattcgagc tgccccaggt
    12481 gatccgcgac cgcctccaac aaccggcggc tccggacgcc gaatccgata acgcgagttg
    12541 accgcgccca tggccttgtt caatccgctc gacaagctca agaattcgct ggcccgcacc
    12601 aaggacaagc tcctcggccg gctcagcgag gttgttcgcc ggcgcgccat cgacgacgat
    12661 ctccttgagg acattgagga gatcctcgtc caggccgatg ttggcctgcc cgccaccgaa
    12721 cgcctcattg cccgcgtgaa agaggccgcg cgggagcggc ggctcaccga gggggaccag
    12781 attatcgacc tgctcaagga ggagatcgtc gcgatcctct cccgcaccgg ccgcccgctg
    12841 tttcccccgg gcgaaccccg gccggccgtt tggctcatcg tgggcgtcaa cggcgtcggc
    12901 aagaccacca ctatcggaaa actggccacg cttttctcgc gcgaggggaa gaaggtcagc
    12961 atcgccgcct gcgacacgtt ccgcgcggcc gcaatcgacc agatttcgat ctgggcggag
    13021 cgcagccgcg tggccctcat ccgcccgcag gagggctccg atccggcggc ggtggctttc
    13081 gacgcggccc gggccgccca tgcccgcggg ctggacctcc tgcttgtcga caccgccggc
    13141 cgtctgcaca cgaagtccaa cctgatggag gagctgaaga agatccggcg cgtgatcgcc
    13201 aaggccgccc ccgaggccgc gatctccacc cgcctcatcc tcgacggttc caccggccaa
    13261 aacgccctgt cgcaggtcag ggtctttacc gacgcggtcg gctgcgacgg cctcattgtc
    13321 accaagctcg acggcaccgc caaaggaggc gtgatgatcg ccgtcgccca ggaactcaac
    13381 gtccccgtcg atttcatcgg cctcggcgag gggatcgacg acatccagcg gttcaacccg
    13441 cgcgagtaca cggaggccct ctttgccgac gagccggcta agcgttgaga cgcacgcgcg
    13501 cgaggagatg atcgacgtca ccgcgcaggt ccgccgcgcc gtccgggaat cgggcgtcgc
    13561 ctcgggcatc gccgtctgct tcgtgccgca cacgaccgcg gcggtcacca tcaacgagaa
    13621 cgccgacccc gacgtggccc gcgacattct ctacaagctg gacaaggaga tcccggcggc
    13681 cgacggctac cgccacgccg aggggaattc cgacgcccac gtcaagagca cgctggtcgg
    13741 ggtctcggcg cacgtgctga tcgagggcgg cgacctggtc ctcggcgcct ggcagggaat
    13801 ctacttctgc gagttcgacg gcccccgccg ccgccacctt atcgtcagag tgatgtccgg
    13861 tgcttaacat caccgttctt gccgtcggca gggacaagga ccgctgggta tcggaggcgg
    13921 tcgcgcacta cgagaaactt cttgcccgct ggtgccgtct cgcctggcgc atcgtgccgt
    13981 cgccgaagca ttcggccgcg ctctctcccg aggagatccg ccgggcggag tcggcgcgcc
    14041 tgcgggcggc gctcggtccc gggtacatcg cggccctcgc ggcctccggc cggacggtcg
    14101 acagcccggc tttcgcccgg ctcctcgagc gctggcacac ggtgtcgggg agcaacctca
    14161 ctttcgtcat cggcggtccc cacggtctcg accgggacct gctctctcgc gccgacacgg
    14221 ttctctccct ctccccgctc actttttcgc accagctcgt gcggctcatc ctgctggagc
    14281 agctctaccg agccttctcc attctccacg ggaccgacta ccacaagtag ggcggtccgg
    14341 ccgcccgcgg agcgcggcct cgtcgggccc ggcgcggcgc ggtcagaact tccggctgcc
    14401 ccgctgggtc agcggcatcg ctttcgcgca cagggggcac tccgccggat cccagctctc
    14461 ggccgccacg gtcgccagcg cggcgaacgg atagtcaaac ctgaccttcc cgttcgaccg
    14521 atcgagcatg agccgcagcc cgacgatctc ggcgccgtac cggcggacca gggcgatcac
    14581 ctcgtccacc gacgttccgg tcgtcagcac gtcatcgaca atcgccacct tctgccccgg
    14641 ctcgaggtga aacccctgct tgaacacccg ccccttgccg tcgtccgccg cctccgcgta
    14701 gatcgcttcc acgccgaggt accgcgcgac atcgtaggcc aggatgatcc ccccggtcgt
    14761 cggcccgaca atcgtccgga cacccgtggt acggagcgtg tcggccatgc gcgcgcacag
    14821 cctggtgcag agcgcgggtt gtttgagcag cgtgaacttt tcatagtaca cgtccgagtg
    14881 ccgtcccgag gtcagcttga aatgcccggt cagaagcgcc ccggactcct tgaacaggtt
    14941 caccacgtca tcctggctca tgaatcctcc gttatgaccg ccacggcggc ggcatgcgtt
    15001 cgctcgtgtg tgattgacag gaaacaccgc gcgcggccca gggccgcccg gatgtcatcg
    15061 ggcagcgaga gcaccggctg tccggaggcg tcgttggtga ttgcgatcgc gcggtaggcc
    15121 gggcgctcgc tcagccggta gccgagggct ttgatgaccg cctctttcgc ggcgaagcgc
    15181 ccggccaaaa acacagactt gtcgtgtcga cgattgagga tggcgagttc ctccgccccc
    15241 aagatgcgtt cggcgaaacg gttgccccag gttcggagat cccgttccat ccgcgcgact
    15301 tccacaatgt ccagtccgat tgcctgaatc atactctttt ccgcggctga ggccggcgag
    15361 ggaagatagg cagcggcgcg atttcccgca acagagtcaa aaaactaatt gcttccggcg
    15421 ccgatgggac gtaccataca agtgacgggg agagagaaat gtaatttgaa aacctcttac
    15481 actggactaa tgaaggtcgt ctcttcgtcg ctgcgcacac gccgtgaccc gtcctttgga
    15541 cggtagcgaa agcaagacgc gggaagtgac tgtaaaaacc ctttaccgta ccgtaatggt
    15601 gtgagtcctt tcaaattccg tggctctcga cccgcaaaag taaaccggcc cactcgtggg
    15661 ccggtttttc ttgttatcgc cgagttcgag tccggctccc gacccgcccc ccgcccggca
    15721 gccgaaaagt tgtaagttgc cgtaccactg cggtcttcgt ataaaacccg cagacagaga
    15781 attactcgag gaaggaatcg atatgaaact gccgtcgctg gcacagcttg cggccgagta
    15841 tggccggacg attcggcgtt ttccccttgt gctggtctgc gcgctgctgg gaaccctggc
    15901 ggccttgatt cttgtggatc aggagggcag ccgcgaggcg tcggttctct acaacatcct
    15961 cctgaccgcc atccttgccc ttcccgtgat gacggccgtg aagctggccg cagagaggag
    16021 cagcgcaccc gcgagcagcg cctggccggc tcaggcggcg gccgccgccc tgctggtggt
    16081 gtacgccgcc accgttccga ccgacctccc ccgcgcacct ctggtgcatc tcctgcgttt
    16141 ctttgcgctg gggatcgggt gcacgctcct ggcctcggtg tggccgttcc ggcagaaagg
    16201 ggaggacaac cgcttctggc atttcaccag cctggtcgtc ttccgaatca tgctcgcggg
    16261 ggcgttcgcg ctggtcctct tcgccggcct ggcgctggcg ctggccgcgc tggacaatct
    16321 cttcggcatg gcgataccgt cgaagcggta tgcggaactc tggatcctta ccctcgggct
    16381 ctttgccgtc ccctatgttt tggccggact gcccgaggat tggagcgcgc tcgggacggg
    16441 gagcgattat ccccggccgc tgaaggtgct ggggcagtat gttctcccgc ctctcgtcgc
    16501 ggtctacttc gtgatcctct acgcctacat cgccaagatc atcgtcacct ggagctggcc
    16561 gcagggctgg gtggggcggc tgatcatcgg cttctccgcg accggcatcc tcgccctctc
    16621 ggtgctcgat cccatgcgcg agaggacgga gtccctgtgg ataaagagag cgtcccgttg
    16681 gttctacctg atcctcctcc ccctcatcgt cgtcctcttc ctggcgctct ggcggcgcat
    16741 ctccgagtac ggtctgaccg aggggaggta tctgggcctg gccgtgggcg tctggctggc
    16801 cgtcatcgcc gcgtacttcc ttctcagccg ttccaagagc atcaggatga tccccgcttc
    16861 cctctgcgcc ttcgcctttt tgatctcagt cggtccctgg ggaatgttca gcgtctcgga
    16921 gcagagccag atccggagac tccgggggat gctgactgcc gattcgatcc tggtgaacgg
    16981 cgcggtgaca aaggcgccgg ccccggttcc cgccgatcac gaggtccaga tcagcgcgat
    17041 tctcatctac ttgtacgagg tgcacggctt ctccgccctg gagccgtggt tcggcgagac
    17101 cctccgcgcg gacacggagg atgccgggaa tcggttcaag gggccggagt acgtcgccga
    17161 acttctgggc atcgagttcc atccatacga tacgcccggg cgggagcagt acttcgcggt
    17221 ccgcattgat ccgcagactc cgatttcggt cgccggatac gaccacatgc tgcgggccgg
    17281 ctacggcggg cgggcggctg cgccgagtcc ggccggcgag ccggtcgccg gcgcaatcga
    17341 gacggccgcc gattccgtgg tcgtgagatt cgtgctcgac agcacgacca ccgattccgt
    17401 caccgtttcg cttcgcccgc tgatcgaccg gatcgtcgcg gcgcgggcag acgcccgcgg
    17461 ttcggagatc tccggcgagg aggcgatgat cgaaaacgaa acgggaggag tcaaggtcaa
    17521 ggtggtcatg ctgcaggcgg acatccgccg ggacgacagc atcaccgtgc tgaactcgta
    17581 cgatgccctg attctctatt cgcgcaggaa gtaacggcgg cgtttggccg gttcgcggcc
    17641 gcgcgccgcg aggtccgggc gggcccgtct ccccgaaaaa aaaagaaggc aggagggtgt
    17701 aaccttctgc ctgtcaatgc tttgcatgga cggatactag acctccatga cctccttctc
    17761 cttgaccgtc aggagagaat cgatctcttt gatgtgctca tcggtcagct tctgcacctt
    17821 gtccaggctc ttgcgcatct catcctcgga gattttcgag tccttctccg cctgtttgag
    17881 atgttcgttc gcgtcgcgcc gaatgttgcg cacggcgacg cgggcctctt ccgccaggtg
    17941 cttgcactgt ttcaccagcg ttctgcgccg ttcctcattg agcgccggga tcggcacgcg
    18001 gatgatttgt ccgtccacca tcgggttgaa cccgaggtcg gcggcctgga tccccttgac
    18061 cacgttgccg accgtcgact tgtcgaacgg cgtcaccacc agcagccgcg gctcgggggc
    18121 cgtcaccgtg gccacctggt tgaggggcat ctgggtgccg taagcttcga cagtaacggt
    18181 gtccagcagg tgcggcgagg cctttcccgt gcggacggtt ccgaattcgc gcttgagcgt
    18241 ctcgatcgtc ttgtgcatcc ggtcgtgggc gtctttctcg atcttgtcaa ccatatcggt
    18301 gcctccctca atggcacgtt gcgtgttatc ccgccgacgt caccagcgtc ccgacgtctt
    18361 ccccgagcac cgtgcggcgg agattgccgg gacggtgcag gtccacgata cgcaggggga
    18421 tttggttttc tttcagaagc gagatcgccg tggcatcgat cacccgcagc tcccgcgtga
    18481 gaatgtccat gtacgacagc cgggggtaga acgtcgcctt cgcgtccttg cgcggatcgg
    18541 ccgagtacac cccgtcgaca ttggtcgcct tgatcatgag gtccgccccc acctccatgg
    18601 cgcgcagcga ggccgcggta tcggtggaga aatacgggct gccggtgccg gccgcgaaga
    18661 tgacgacccg ccccttctcc atgtgccgca ccgcgcgccg ccggatgaac ggttcggcga
    18721 aggcttccag tttgaccgcc gacataacgc gggtgtagac gcccatgtgc tcgagcgtgt
    18781 ccatcatcgc cagggcgttg atcacggtgg cgagcatccc catgttgtcg gcggtcaccc
    18841 ggtccatgcc gagctcggtg gccgccaggc cgcggaaaat gttcccgccc ccgaccacga
    18901 tgccgatctc gcaccggagc tgcttgactt ctatgatttg gccgcagacg gcttcgacgg
    18961 tggggctgtc gatgccgaac tcgcgggcgc ccatgagcgc ctcgcccgag attttgatga
    19021 gaacccgctt gtataacggc gcgtctgtgt ccatacgcta ctcaccgagc cggaaccgtg
    19081 cgaagcgccg caccttcatg ttctctccca gccgcgcgat cgtctccttg aggtactcct
    19141 cgatcgtctt gtcggcgtcc ttcacgaacg gctgctccat gagcacgact tcgccgaagt
    19201 atttctcgat ccggccgtcg acgatcttgt cgacgatctt ctccggcttt ccctctttga
    19261 gcgtctggtg ccggtagatt tcgcgttcct tggcgatgag cgccggatcg acctcgtccc
    19321 ggctcaccac caggggggcg ctggccgcga tgtgcatggc gatgttgcgc gcgaattctt
    19381 tgagctggtc ggtccgcgcg acgaaatcgg tttcgcagtt gacctccacc atcacgccga
    19441 gcttgtcgcc cgggtggacg taggtggcga tgacgccttc ggcggtcgcc cggccctcct
    19501 tcttggccgc cttggagatt ccctgctcgc gcaggtagtc gatcgctttg ctgaggtccc
    19561 cgtgcgtctc ggtcagggcc ttcttgcagt ccatcatgcc ggccccggtt ttttcccgaa
    19621 gctctttcac cagttgtgcg cttatgtcca tgtgtcgaac cttcttactc agcttggtct
    19681 ttattcgcga cgtcgtgtcg gccggccgcc ggggagtggc cattacccgg cgctccttca
    19741 cgctccgacg ccgcgagcat ctcggtcacc accgtgctct tggcctccac cgccgcgtcg
    19801 accagcgcgt gcatcaggat ccggatcgac ttgatcgcgt cgtcgttgcc ggcgatcggg
    19861 aaatcgatca ggtcgggatc ggcgttggtg tcgacgatgc cgatgatggg gatccccagc
    19921 ttgtgggcct cggccaccgc gattcgctcc ttgcgggcgt cgacgatcac gaccagcccc
    19981 gggaggtggc ccatctcctt gatcccgccg aggtacttct ggagctttcc cgcctcgtgc
    20041 tcgatgcggg agcgttcttt cttggagaac ttctcgagcg tcccgtccac ccgcatcttc
    20101 tcaatatctt tgagtttttt gaccgagtgc ttgatcgtct ggaaattggt cagcatgccg
    20161 ccgagccagc gttcggtgac gtagtagccg ccgcagcggg cggactcttc cttgatcacg
    20221 tcctgggcct gttttttggt tccgatgaag aggacggatt tccccttgcc caccgtttcc
    20281 gccattttgc ggcgggcctg ttccagggcg ttgacggttt tctgcaggtc gatgatgtag
    20341 atgccgttgc gggcggcgaa gatgaacggc ttcattttgg ggttccaccg gcgcgtctga
    20401 tggccaaaat ggaccccggc ctcgagcaaa tcctttacct ggggactaac catggaatgc
    20461 cttcgtgttg agcggttagg tctttacccc gacatcgcct cgacccggac ccgcggattc
    20521 ggggaccgcc ggatcgatcc gtcgggattg tatattttcc cgccgaggcg ggaatggcaa
    20581 atcttcgtac ccggcttagc gcttcgagta ctggaaccgt ttgcgggctt tcgggcggcc
    20641 gtacttcttg cgctccacct cgcgggggtc gcgcgtgagc aggccgttct ggcgcagcgt
    20701 cggccggaaa tcttcattca gacgggccag cgcccgggcc accgccagcc gcaccgcccc
    20761 cgcctggccg ctcagtccac ccccgctggc ccggcac
//
LOCUS       DAOPOE010000056        20375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3241_length_20375_cov_5.244439, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000056 DAOPOE010000000
VERSION     DAOPOE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20375
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_20375_cov_5.244439"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2515)
                     /locus_tag="PKM94_08615"
     CDS             complement(<1..2515)
                     /locus_tag="PKM94_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD66738.1"
                     /translation="MNISSLCISRPVLATVLSILIVLFGGIGFFFLGVREYPNVDPPI
                     ISVSTAYTGANADVIESQITEPLEEEINGISGIRSLTSTSRDGRSSIRVEFELGVDME
                     AAANDVRDRVSRALGSLPKDVDPPIVSKADADATPILSMTLQSDRRSLLELSAIANDE
                     FKERLQTIPGISEVGIWGEKRYAMRLLIDPGRLAAHGLTPLDVRDALNRENVELPSGM
                     IEGYATELSIRTFGRISTPAEFNDLIIRETAGSVVRLRDVGEAILAPENERTSLRGNG
                     GIPMVAVAITPQPGSNHIAIVDEVYRRVEQIARELPDDLTLNIAMDTTASIRRAITEV
                     GETILIAILLVLLVIFVFLRHWRTTIIPMLAIPISLIGTFFIMYAAHFSINILTLLGV
                     VLSTGIVVDDAIVVLENIYSKIERGMPPVEAGHRGSREIFFAILSTTITLASVFLPII
                     FLQGLTGRLFREFGIVVAGAVLISAFVSLTLTPTMSAHLLHKSEHENRLFLWSERRFA
                     ALAAGYRRLLGRFIERRLLAPVIMAAALALIFGIGATIPSELAPMEDRSRLVINATAP
                     EGTSFDAMSAYIDRLIGIVDTLPERDVIMAMTAPGWGGGGSANSGFVRVNLTPPDRRA
                     RTQQEIADAIGARLRAETFARAFVTQEQTIGSGRGPGLPVQLVIQAPTLEKLKAAIPP
                     FMERAQTDSVFQVVDLNLRFNKPELTVGIDRDRARALGITVRDIAETLQLYFSGQRYG
                     YFIMNGKQYPVIAQADRPNRDEPLDLSSIYIRNNRGELIQLDNVVTLSYRSSPPQLYR
                     YNRYVAATVSAAPAPGVALGTAIDEMQGIANSVLDESFST"
     gene            complement(2512..3594)
                     /locus_tag="PKM94_08620"
     CDS             complement(2512..3594)
                     /locus_tag="PKM94_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66739.1"
                     /translation="MSIRKTSNWCLLWAAVAAAAGCGDSPGDDAGRSGGNPGGAAMPV
                     NVIVVQPQPLDNKIFTTGTLLANEEVALRPEISGRVVGLYIEEGKAVKKGDLLVKLND
                     RELEAQLRRKQHEEALAAEEERRANALLDIKGISQEDYDRTLNKLRMIQAEREVIEAQ
                     LAETEILAPFDGTVGLRYISAGGVVTPSTLVATMQDTDPVKVEFSIPEKHAGKLAVKT
                     PVLVQVGDAPERHEGTVYAVEAKVDPATRTLKARATVPNPDGRLIPGSFAKVEITLEH
                     LPDALIVPAQAIVPQLGGDLVYVCREGAARATPVTVGLRGERTAQIIQGLAPGDTVIT
                     TGLLQLNDGKKVAARPEPADRSRTGL"
     gene            complement(3692..4099)
                     /locus_tag="PKM94_08625"
     CDS             complement(3692..4099)
                     /locus_tag="PKM94_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66740.1"
                     /translation="MRKLVLFVVVVASLTGGPSRPAAGVIPSWESRTGKLGFMPLSNE
                     VTMLRSVDDAGGDRGWTLQLVLINSLKVFTTFKLEFTADYNFDYTPGQNRDHYVELSL
                     VKPVTGLVSLNYQRVLSTFEAEAVNQFGVRLAF"
     gene            complement(4214..5290)
                     /locus_tag="PKM94_08630"
     CDS             complement(4214..5290)
                     /locus_tag="PKM94_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD66741.1"
                     /translation="MATHKRILVTGASGSLGRQLLYEFTRRGCRPIAHVRPDSDTSYI
                     DSLGLEKRVADLGNEGQIEALVRDVDGIIHTAAWVNFRQDRLTQFAGVNTFGAVHVFQ
                     AAQKAGVERFVHVSTIAATAAIPRKNGEDGRPGGPRVRVTEETPFNLGHLRIPYFMTK
                     RAAEEELLKLAAGGATQLVIVNPAIIVAPSSTGDDRAKALKRFPRLVMPGFPNRLNLV
                     DIRDVAPAIIAALERGRPNERYILAGDNITARELVLSISQALGRVPHVMHVPRGVIEV
                     AARLWGIWVKLTGRGKLAFYPDLVKLLDYDWAYSSLKARRELGYTSRSVWVTLDDLLH
                     NRFLGTYLRPATEGKSEAAEKTPS"
     gene            5398..5643
                     /locus_tag="PKM94_08635"
     CDS             5398..5643
                     /locus_tag="PKM94_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66742.1"
                     /translation="MKKVASYLWGRWRRAARVLGRAQTLALLAVLYALLLSPLGLLMR
                     LGGWDPLDVSARKAARTTNWKPVARPAPDREALRRQS"
     gene            5645..7378
                     /locus_tag="PKM94_08640"
     CDS             5645..7378
                     /locus_tag="PKM94_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOD66743.1"
                     /translation="MNILGLSCFYHDAAAALAVDGRLVAAAQEERFSRLKHDPELPVR
                     AVRWALDRTGLSTADLDAVAFYDKPILKFDRILTGCLAAPFRSWRPFLTALPVWLGRK
                     LWTDHIIRKELGFDGEVLYLPHHLSHAAGAFFTSPFSDAAVLTVDGVGEWATASYGVG
                     EGNRVRLIAEMHYPHSLGLLYSALTYYLGFRVNSAEYKVMGLAPYGKPRYADLIEDRL
                     VTVHADGSLHLRMKYFTFHRSLTMTGRAIERLFGCPRRAPGAPLEQFHADVAASLQAV
                     TEKIVLAMARHVRETTGKSRLCLAGGVALNCAANGALLRSGLFEDVAIQPAAGDAGGA
                     VGAALYAHFCLTGAARQPQPFFGIGPNFDHAIAPFLAAHRIPHTAGDRAAQLRRVAAA
                     LASGRIVGFYHGGAEFGPRALGFRSILADPRDPRMKEKINAAVKFREPFRPFAPAVMC
                     EHAADYFECDRPAPHMLVNFTVRADKRSLIPAVTHVDNSTRIQTVAEADNPPLYRLLA
                     EFKSLTGIPVLLNTSFNLRGHPIVNTPEEAFATFCSGGLDILLLGDYLVKKEEIPEAL
                     RQRFRLVQAED"
     gene            7424..7585
                     /locus_tag="PKM94_08645"
     CDS             7424..7585
                     /locus_tag="PKM94_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009543764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOD66744.1"
                     /translation="MAGRVKILAEFWYFLRTSRKWWLGPILVLLVLVSALILFTESSA
                     LAPFIYSLF"
     gene            7644..8684
                     /locus_tag="PKM94_08650"
     CDS             7644..8684
                     /locus_tag="PKM94_08650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOD66745.1"
                     /translation="MPPRDTYPVLFRTAAVLLSLALFVALAEGVVRLAGLDPYFQNRF
                     FTVNRALDYPEIFDKDHELFWRFRPDQSVTSRFFIGRTHRINSAGLHGPEITPAEGRT
                     RVITLGNSCTFGWGVATEHGYPRLLDSLLGDSFQVINAGIPGYTSLQGKRFYRSDLSR
                     LRPAIVTILFAFNDHWAAAGDRADKDQRMPPQWLLDIQNALGRLHTYRLLKKAILSAV
                     EPPMDSLFDRRTPIYRVGLEDFRANLEDLCRMIVADSARPILLTSPIPDLPTYYPKGY
                     QSNLHKYHERYNAAIREMAAARGYDVVDLAEGFAGRPGLFDDPLVDPAHFNDFGHRLA
                     AAMIAACIRDSL"
     gene            complement(8685..9854)
                     /locus_tag="PKM94_08655"
     CDS             complement(8685..9854)
                     /locus_tag="PKM94_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD66746.1"
                     /translation="MTTSTAEQFRAVRGEFPHTRELVYFNSASYGPYSTRVQRALAET
                     IAARVRSAEDSTASIFAMREALRRDYGALIGARPSQIGIGLNTTFGLNVAAYGLPLKK
                     GDEVLLTDVEFPALVYAFRGAAQTRGITLRFVKTRDRRVDVEQLSRAVTKRSKVLAIS
                     WVQFFDGYRNDLAALGEFCRDHGLFFVVDGIQGTGMQPLDVRKLGIDIFSTGCQKWLL
                     APQGSGFFYIADRIRDRLQPPSASWLGVEWHQKFGDLFRYDREFLATAARFELGYYVE
                     LNLAGMRESAAMFRELGTRNIQRHTRALIDRLADYIRGHKFYRITSSMEPKHRSSIFT
                     FTCDRFRDLHKELYRRKITCVHREGSIRISVHFFNNEDDIDKLIAVLDRFAESAP"
     gene            complement(9862..10281)
                     /locus_tag="PKM94_08660"
     CDS             complement(9862..10281)
                     /locus_tag="PKM94_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66747.1"
                     /translation="MINMTLMAYCLIAGGVYLLVVTLAHEWLGRKLDMAKGIPPAQRE
                     AWGPAAFVVGYVMEGLFFVAIPTLAYSFFTAVLPLAGIRTGLALALTGFVLGGVPAIM
                     GLSLRVRLWMPHLLYFLLGLLLKLGGCLAIIGYLYSL"
     gene            complement(10278..11273)
                     /locus_tag="PKM94_08665"
     CDS             complement(10278..11273)
                     /locus_tag="PKM94_08665"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD66748.1"
                     /translation="MSETKATVLVTGANGFVGSRLCRALQAAGYRVIAGVRKTADVSL
                     LKDVPVEYRFGDLSTVEGLRPMVAGTDYVVHNAGAVKAKRAATYFEVNEAGARRVCEA
                     VIAEQAPVRKLVYISSLAAAGPSTDGRPVTEADEPQPITTYGRSKLAGERAVLSFADR
                     FHVISLRPAAVYGPGDRELLTFFAAAHRGLRPVIGDPRRKLQLVHADDLSRAVVMALT
                     GGTRAGEAYFIAEEEAYAMGRLVDLVAAAGGRKGIGLRLPGGVFRAIAAVSEGLFRLA
                     GATPMLTREKCRELLASWEVSTAKARAEFGFQAQIGFADGARETFAWYRREGWLT"
     gene            complement(11630..12694)
                     /locus_tag="PKM94_08670"
     CDS             complement(11630..12694)
                     /locus_tag="PKM94_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66749.1"
                     /translation="MKRVVFLSAVALLFLAAQALLNCSSPLETLDNGNPPRPEPEPPD
                     TIFLWDTIIVIDSAAVETLFRVDTLLEFDTVTLIDTVHWYDTTFLVDTTTLYDTVIQV
                     DTTEFWDTITLVDTTIQYDTVIVLDTIATVDTIIQVDTLNNYDTTFIVDTLIEVDTVT
                     WVDTLTEYDTLLVVDTLVQTDTLLAVDTVIVTDTLVHVDTVTTVDTLELVDTLVVADT
                     IIQVDTVIQTDTVIVTDTIIQTDTLTIVDTVYVVVPDTILTSHCANLGAGQKEIIWLL
                     SNEAGPYRLEFRGLAEREHPSNVVKITIGGAEYLWDVWNQPEFVVERELAADATIRIE
                     RIKPNPYGHGIDICVTVRPN"
     gene            complement(12691..13659)
                     /locus_tag="PKM94_08675"
     CDS             complement(12691..13659)
                     /locus_tag="PKM94_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66750.1"
                     /translation="MKRAFLTMVALGLVLGATAGAQEVVIPGFPEAVGGSIGEEFFQP
                     YYADLQALADTLAQYPLMLAVVTGGADGLEYREHHDAKNPGLAIGRAHVLRNLLVDKF
                     KIDPHRIIIQSVDVLAAGEAHRFASVRVVRELSDLGGRVSALEERPPVEKHFTETVRE
                     VPGEAPALDDFLGLQFGAGVSTSPFGGLPIVTGALSWKRTVYLEGVFGYTLWNSEFQF
                     EDQLLKTKRRLAGGQVVVFPLKNIPVGAVGGWMRFEEISEYYYKYVRLSEGPMVGVRA
                     VPFDHLAITGAYNPARHRITEDLKSMAKNGQFMLTLTAFVEIGGAR"
     gene            14015..15388
                     /locus_tag="PKM94_08680"
     CDS             14015..15388
                     /locus_tag="PKM94_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOD66751.1"
                     /translation="MTNEKSISQKMAEFAVELRYDDLPEDVVHEVKRYLYDSVGCAYG
                     SYHTKDVNIIRDLCREMGGRPEATVIGFGDRLPAVNATLVNSLMIRALDFNDIYWRED
                     PSHPSDLIPAALSVGELVDAPMRDVIVAIVLAYEFEQRLCEFAVPGVRERKWHHATLT
                     QFVSPIVAGKVLGLTAAQMANAIGINGCHNHTIGCPTAGKLTMMKNTVDPMAVQAGVF
                     AALMAGKGYTGTTAVFDGKEGLMDVFGPEWDLHRLLGHLGEKYKILECGMKAFPTEAL
                     THTHLTCLLKVVTENNITYDQVEEVIVTTIARACDILFDPHKYRPDSRETADHSLPYC
                     LAAALVDHEISVQSFSEKKLKDERIWQVIDKIKGEASTEFEAMFPAKQPSRVVVRTRD
                     GRSYSAYLEYPKGDPREPMTMHDLEVKFKGNSAALLAPERQTAVRDAIFACEKLSCRD
                     LMAKLVV"
     gene            15450..16274
                     /locus_tag="PKM94_08685"
     CDS             15450..16274
                     /locus_tag="PKM94_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOD66752.1"
                     /translation="MIADHRHSLRRPRGVFDFAALAERPVPAIFAHRGASRLHRENTL
                     RAFTAAIALGADGIEVDVRRTRDGVLVVHHNARPLGSSRRIASLDYDALLELARRRAY
                     HVPTLEETLDLCAGRAALDLELKESGYEAEVVRLVRRRYDLRHVAFTSFRGPVVAALR
                     KAAPRAALGLLVGPAAPGTLRLALRRSALARKIDRVGADFIAPHRRLVTRAFSARMEE
                     LGVPVAVWTVNTLPAAQRLTRRGVAILITDLPGTIGPAVRSLGEKGARGEPPREDL"
     gene            16274..16633
                     /locus_tag="PKM94_08690"
     CDS             16274..16633
                     /locus_tag="PKM94_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66753.1"
                     /translation="MKPHYLLILLALAMLAGGAVGLARTGSPIPLLINSALALLTLIL
                     ALVVSRDSRRVLLAATVWVALCVLLYTYMTIIPPAAHNPSRPGSKYLFGSMALVALIV
                     LVGLSGGLRRRRSQRRL"
     gene            16838..17428
                     /locus_tag="PKM94_08695"
     CDS             16838..17428
                     /locus_tag="PKM94_08695"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOD66754.1"
                     /translation="MRNRILEAALMEFAERGVAGARVDRIAARAGVNKALIYYYYTSK
                     QRLYEESISSYMRALLDSVRLSLDEASNLEEALHGVAERYSSIFLTRPEIPRLMMREL
                     ANPGNTLVSQWAERITASGVPATIRRRLEAGREAGEVRPIDLRQAFVSFLTMQIGYFL
                     MSPLVDRVLHLEDREAFVVARRDAVVDLFLNGVKAR"
     gene            17425..18726
                     /locus_tag="PKM94_08700"
     CDS             17425..18726
                     /locus_tag="PKM94_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD66755.1"
                     /translation="MRRTLLAGLLAVLAAGPSPAARQLTLREALTLAREHSFEIRRAL
                     AEEQAAGEALGAARAARLPTLDASAIAFYNTEVASLDLATSLGRLSREIGTKENVQTD
                     LRLAFPLYTGGRIAGGVDAAQASARRQAALTEAAVDDLLYRTRVAYLTVHRSDRQIEA
                     ARSALRRAQITRAQVASLLSAGAADSVAVLEAQLAVSETELRLQQALHDRREAVIQFA
                     QLVGLSASDSLDLATPLAPPAAPGDPDTGFVRPELAAAQAAVEIGRAGVARAAADWFP
                     TLAAFGGYSYGKPNLDRFGNTWNDYWQVGARLDWSFNLGRGTRREVNRAKFTVSAAEN
                     QRNRLAEQLREQHLLAWERLRLAYEQYLTALDRAALTRRNYELATSRHSEGVLSSNRL
                     MEIEATLNEAEAGLAAAEAGYYIARSAYLYASGADLMTEGF"
     gene            18728..19609
                     /locus_tag="PKM94_08705"
     CDS             18728..19609
                     /locus_tag="PKM94_08705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66756.1"
                     /translation="MRLLLFTTALLGLLLAAGCRNEDAALSGSGMIEATEVTVSAQVG
                     GQVEKLYFDEGTVIAPGDTLLVVDTTALGLELASARAGRALARTRLQAAQLRLNDALV
                     TEAYLANEVKRIETLVAANTATRRQLDELQYRHTQAGIAVGSARSAIASAEAEIAKID
                     ADIARLLDQWDDAFPVAPIAGTVTEKYVDAGELLAPGRPIARIARLDTVWVKIYLPAR
                     EFAAVTIGAAATVDTEAGESSPGRVTWVADQAEFTPKNVQTKKARADLVYAVEITVAN
                     PAHKLKIGQPVFVTVGE"
     gene            19609..>20375
                     /locus_tag="PKM94_08710"
     CDS             19609..>20375
                     /locus_tag="PKM94_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66757.1"
                     /translation="MAFLEVQGLARSYRGVRAVEDFSFAMERGEIYALVGPDGAGKTT
                     VIRAIANLITPDRGRILLDGTDIRDRFDRVKSRLGYMPQVFSLYPDLSVEENLIFYAG
                     VYGVTGRAYEERRDRMYDFSNLRPFAGRRAAALSGGMKQKLALSCALMHDPELLLLDE
                     PTTGVDPLSRRQFWDILLQLKRDGAAVLVTTPYMDEVARADRGSFVFGGRKLAEGSPR
                     ELTESFSGEVYFVGAAADLELVRRLNELPEVTAQRFGA"
BASE COUNT         3365 a         7204 c         6251 g         3555 t
ORIGIN      
        1 tggtggagaa cgactcgtcc agcacgctgt tggcgatccc ctgcatttcg tcgatagccg
       61 tcccgagcgc tacgccgggc gccggggctg cgctcacggt ggccgcgaca taccggttgt
      121 accggtacaa ctgcggcggg ctgctccggt aggagagcgt caccacgttg tccagttgga
      181 tcagttcccc ccggttgttg cggatgtaga tggagctgag gtccaggggt tcatcgcggt
      241 tgggccggtc ggcctgcgcg atcaccgggt actgcttgcc gttcatgatg aagtagccgt
      301 agcgctggcc gctgaaatac agctggaggg tctcggcgat gtcgcgcacc gtaatcccga
      361 gggcccgggc gcggtcacgg tcgatcccga cggtcagctc cggcttgttg aagcggaggt
      421 tgagatcgac cacctggaaa acggaatcgg tctgcgcccg ctccatgaac ggcgggatcg
      481 ccgccttgag cttctccagc gtcggcgcct ggatgaccag ttgcacgggc agtcccgggc
      541 cgcggccgct gccgatggtc tgctcctgcg tgacgaacgc ccgggcgaac gtctccgcgc
      601 ggagccgcgc gccgatcgca tcggcaattt cctgctgggt gcgcgcgcgc cggtcgggcg
      661 gcgtgaggtt gacccgcacg aagccgctgt tggccgagcc tcccccgccc catccggggg
      721 ccgtcatcgc catgatcaca tcccgctccg ggagcgtgtc gacgatgccg atcaggcggt
      781 cgatatacgc gctcatggcg tcgaacgatg ttccttcggg ggcggtggcg ttgatcacca
      841 agcggctccg gtcctccatg ggcgcgagtt ccgaggggat tgtggcgccg atgccgaaaa
      901 tgagcgccag ggcggcggcc atgatcaccg gggcgagcag tcgccgctca atgaaccgtc
      961 ccagcagccg ccggtagccc gccgccagcg cggcgaaccg ccgctcgctc cagaggaaga
     1021 gccggttttc atgctcgctc ttgtgcagca ggtgcgcgct catcgtcggc gtcagcgtca
     1081 gcgacacaaa cgccgaaatg agcaccgccc cggccaccac gattccgaat tcccggaaca
     1141 gccgccccgt cagcccctgc aggaaaatga tcggcaggaa caccgaagcc agcgtgatcg
     1201 tggtcgacag gatggcgaag aatatctccc gcgacccgcg gtggcccgcc tcgaccggcg
     1261 gcatgccccg ctcgatcttg ctgtagatgt tctccagcac cacgatcgca tcgtcgacca
     1321 cgatccccgt cgacagcacg accccgagga gagtaaggat gttgatcgag aagtgagccg
     1381 cgtacatgat gaagaaggtg ccgatcagcg agatggggat tgcgagcatc gggataatcg
     1441 tcgtccgcca gtgccgcagg aacacgaaga tcaccagcag gacgagcagg atcgcgatca
     1501 ggatcgtctc gcccacctcg gtgatggcgc ggcggatgct cgcggtcgtg tccatcgcga
     1561 tattgagcgt gagatcgtcc ggaagttccc gggcgatctg ctccacccgc cggtagactt
     1621 catcgacgat ggcgatgtgg ttggaccccg gctggggtgt gatggccacc gcgaccatcg
     1681 gaatgccccc gttgccgcgc agactcgtgc gctcgttttc cggcgcgagg atcgcttcgc
     1741 cgacgtcccg caggcgcacc accgagccgg ccgtttcccg gataatcagg tcgttgaact
     1801 ccgccggcgt cgatatccgt ccgaacgtgc ggatcgacag ctccgtcgcg tacccttcga
     1861 tcatccccga gggcagttcc acgttttccc ggttgagggc atcgcgcacg tcgagcgggg
     1921 tgaggccgtg ggccgccagc cgccccggat cgatcagcag ccgcatggcg taacgtttct
     1981 cgccccagat tccgacctcg ctgatccccg gaatcgtctg cagccgctcc ttaaactcgt
     2041 cgttcgcgat cgccgacagt tccaggagcg accgccggtc gctctggagc gtcatcgaga
     2101 ggatcggcgt cgcgtcggcg tccgccttcg acacgatcgg cgggtccaca tctttgggca
     2161 ggctccccag ggcgcgcgac acgcggtccc ggacgtcgtt ggccgccgcc tccatgtcca
     2221 ccccgagctc gaactcgacc cggatcgagc tccgcccgtc gcggctcgtc gacgtcagcg
     2281 accggatccc ggaaatcccg ttgatctcct cctcgagcgg ctcggtgatc tgcgattcga
     2341 tcacgtcggc gttggccccg gtgtacgcgg tcgagaccga gatgatgggc gggtcgacat
     2401 tcggatactc ccgcacgccg aggaagaaaa agccgatccc cccgaacagc acgatcagga
     2461 tcgagagcac ggtggcgagc accggccggc tgatgcacag ggaggaaatg ttcatagtcc
     2521 ggtccggctg cggtcggccg gctcgggccg cgccgccacc ttcttcccgt cgttcaactg
     2581 cagcagcccg gtggtgatga cggtgtcgcc gggggcaagt ccctggatga tctgggcggt
     2641 gcgctctccc cgaaggccga ccgtcaccgg cgtcgcccgg gccgcgccct cgcggcacac
     2701 atagacgagg tcgccgccga gctgcgggac aatcgcctgg gccggcacaa tcagggcgtc
     2761 ggggagatgc tccagcgtaa tttccacttt ggcgaacgac ccggggatca gccgcccatc
     2821 cgggttcggc acggtcgccc gcgctttcag cgtccgcgtc gccgggtcca ctttggcctc
     2881 gaccgcgtac accgtcccct cgtggcgctc cggcgcgtcg ccgacctgca ccagcaccgg
     2941 cgtcttcacc gcgagcttcc cggcgtgctt ctccggaatc gagaactcca ccttgacggg
     3001 gtcggtgtcc tgcatcgtgg ccaccagcgt gctcggcgtc accacgcccc cggcgctgat
     3061 gtagcgcagc ccgaccgtcc cgtcgaacgg cgcgaggatc tccgtctccg cgagctgcgc
     3121 ctcgatcacc tcgcgctccg cctgaatcat ccggagcttg ttcagcgtgc ggtcgtagtc
     3181 ctcctggctg atccccttga tatcgaggag ggcattcgcc cgccgctctt cttcagccgc
     3241 cagcgcctcc tcgtgctgct tgcgcctcaa ttgcgcctcc agctcgcggt cgttgagttt
     3301 caccagcagg tccccctttt tcacggcctt cccctcctcg atatacaggc cgaccacccg
     3361 cccggaaatc tccgggcgca gggcgacctc ttcgttggcc agcagggtgc cggttgtgaa
     3421 gattttgttg tccaggggct ggggttgcac gacgatcacg ttcacgggca tcgccgcgcc
     3481 gcccgggttt ccgccggatc ggccggcgtc gtcgcccggg ctgtcgccgc acccggccgc
     3541 cgcggcgaca gccgcccaaa gcagacacca attgctcgtc tttctgatac tcatactttt
     3601 gtcatacact caccggtttt gaatcgcacc aatatatgcg cgcccgagac cctcagaaag
     3661 gcgaaaacac tcccggccgg cccgcacccg atcagaacgc cagccgcacc ccgaactggt
     3721 tcaccgcctc ggcttcaaag gtcgacaaca cccgctggta gttcagcgag accagcccgg
     3781 tcaccggctt gaccaacgac agctcgacat agtggtcccg gttctgcccc ggcgtgtagt
     3841 cgaaattgta gtcggcggtg aactctaact tgaaggtcgt gaacacctta agcgaattga
     3901 tcaagaccag ttgcagcgtc cacccccggt cgccgccggc atcgtcgacg ctcctgagca
     3961 ttgtcacctc gttggacaga ggcatgaacc cgagcttccc ggtgcggctc tcccatgagg
     4021 gaataacccc tgcggcggga cgggagggcc cgccggtcag ggaggcaacc acgacaacaa
     4081 acagcacaag cttgcgcatg gcctgctcct tcggcttgag atggtacggc ggcgcctcgt
     4141 ctgatacgaa gcccgccggc gtacggatgt cagggaggca atataaacaa acggcggctt
     4201 ccccgccaag gcctcacgag ggcgtcttct ccgccgcctc ggatttcccc tccgtcgccg
     4261 gccgcaggta ggtcccgaga aaccggttgt ggagcagatc gtccagcgtc acccacaccg
     4321 agcgcgaggt gtacccgagc tcgcgccgcg ctttgagcga ggagtacgcc cagtcgtaat
     4381 ccaggagttt gaccagatcg ggatagaacg cgagcttgcc ccgtccggtg agcttcaccc
     4441 agattcccca cagccgggcc gccacctcga tcaccccgcg cggcacgtgc atgacatggg
     4501 gcacccggcc gagcgcctgc gaaatgctca gcaccagttc gcgggccgtg atgttgtccc
     4561 cggccaggat ataccgctcg ttcggccgcc cgcgctccaa agcggcgatg atcgccggtg
     4621 cgacatcccg aatgtccacg aggttgagcc ggttcggaaa ccccggcatc accagccggg
     4681 gaaaccgctt cagcgctttc gcccggtcgt cgcccgtcga cgacggcgcg acgatgatgg
     4741 ccggattcac gatcaccagc tgggtcgccc cccccgccgc cagcttcagc aactcctcct
     4801 cggccgcccg cttcgtcatg aagtacggga tgcgcaggtg cccgagattg aacggcgtct
     4861 cttccgtcac ccgcactcgc ggtcctcccg gacggccgtc ctccccgttt ttccgcggaa
     4921 ttgccgccgt cgccgcgatc gtcgacacgt ggacaaaccg ttccaccccc gctttctgcg
     4981 ccgcctggaa gacgtgcacg gctccgaagg tgttcacgcc cgcgaactgg gtgagccggt
     5041 cctggcggaa attgacccag gccgcggtgt ggatgatccc gtcgacgtcc cgcaccagcg
     5101 cctcgatctg cccctcgttg ccgaggtcgg caacccgttt ttccaggccg agcgaatcga
     5161 tgtacgaggt gtcggagtcg ggccgcacgt gggcgatcgg ccggcatccc cggcgcgtga
     5221 actcatagag caactgccgg ccgagcgagc cggaagcacc ggtgaccagt atgcgcttgt
     5281 gcgttgccat ctgcttcgtg agctaattgg gagccgcggc gtcggccgcc ggacccccgg
     5341 aaatacgcaa agaatctcga ctctcacaac agcttttatt agattaacgc gcggaaaatg
     5401 aagaaagtcg catcgtatct ttggggccgg tggaggcgcg ccgcccgcgt gctcggccgg
     5461 gcacagacgc tggcgctgct ggccgtcctc tacgcccttc tcctgtcgcc gctcggcctg
     5521 ctcatgcgtc tgggcggatg ggatcccctc gatgtttccg cgcggaaagc cgcccgcacc
     5581 accaattgga aaccggtcgc ccgccctgcg cccgaccgcg aggcgctccg acggcagagc
     5641 tgacatgaat atcctcggcc tatcctgctt ctaccacgac gccgcggcag cgctggcggt
     5701 cgacggccgt ctcgtggccg ccgcccagga ggagcgtttc agccgcctca agcatgatcc
     5761 cgagctgccg gtccgcgccg tgcgctgggc cctcgaccgg accggtctgt cgacagccga
     5821 tctcgacgcc gtggccttct acgacaaacc gattctcaaa ttcgaccgca tcctgaccgg
     5881 ctgtctcgcc gcccctttcc gctcctggcg cccgttcctg accgccctgc cggtctggct
     5941 cggccgcaag ctctggaccg accacatcat ccgcaaggaa ctgggatttg acggcgaggt
     6001 gctctatctc ccgcaccacc tctcgcacgc cgccggcgct tttttcacct ccccgttcag
     6061 cgatgccgca gtgctcacgg tcgacggcgt cggggagtgg gccacggcct cctacggcgt
     6121 gggcgagggc aaccgggtcc ggctgatcgc cgagatgcac tacccgcact ccctcggtct
     6181 gctctattcg gccctcacct actacctcgg ctttcgcgtc aactcggcgg agtacaaagt
     6241 gatgggtctc gccccgtacg gcaagccgcg ctatgccgac ctgatcgagg acaggctcgt
     6301 caccgtccat gccgacggct cgctccacct ccgaatgaaa tacttcactt ttcaccgcag
     6361 cctgaccatg accggacggg ccatcgaacg tcttttcggc tgtccgcgcc gcgcgcccgg
     6421 cgcgccgctg gagcagttcc atgccgacgt ggcggcctcg ctccaggcgg tgacggagaa
     6481 gatcgtcctg gcgatggcgc ggcatgtgcg cgagacgacg ggcaagtccc ggctgtgcct
     6541 ggcggggggg gtcgctctca actgcgccgc caacggcgcc ctgctgcgct cggggctttt
     6601 cgaggacgtc gccatccagc cggccgccgg cgatgccggg ggggcggtcg gcgcggccct
     6661 gtacgcccac ttctgtctca cgggcgccgc tcgccaaccg caaccctttt tcgggatcgg
     6721 cccgaatttc gaccatgcga tcgccccctt tttggccgcc caccgcatcc cgcacacggc
     6781 gggcgatcgg gccgcccagt tgcggcgcgt cgccgccgcg ctcgcctcgg gccgcatcgt
     6841 cgggttctat cacggcggcg ccgagttcgg accgcgcgcc ctcggcttcc gttctatcct
     6901 ggccgatccc cgcgaccccc gcatgaagga gaagatcaac gcggccgtga aattccgcga
     6961 accgttccgc cccttcgccc cggcggtcat gtgcgagcac gccgccgact acttcgaatg
     7021 cgaccgccct gccccccaca tgctcgtcaa cttcaccgtc cgcgccgaca agcgcagcct
     7081 gattcccgcg gtcacccatg tcgacaactc cacccgcatc cagacggtgg ccgaggccga
     7141 caacccgccc ctgtaccgcc tcctggccga attcaaatcc ctcacgggaa ttccggtcct
     7201 gctcaacacc tcgttcaacc tgcgcggcca cccgattgtc aacacccccg aggaagcgtt
     7261 tgcgaccttc tgctcgggcg gtcttgacat tctccttctg ggtgactatc tagtgaagaa
     7321 agaggagatt cccgaagcgc tgcggcagcg tttccgtttg gtgcaggcgg aggactgaag
     7381 tgtctgcgcg ccgcaggagt aatctcccgt gaggcaatcg gttatggccg gacgagtcaa
     7441 aatcctcgcc gagttctggt acttcctccg gacctcccga aagtggtggc tcgggccgat
     7501 cctggtattg ctcgtgctgg tgtcggccct gatcctcttc accgaatcct ccgctctcgc
     7561 cccctttatc tactcgctct tctgaccgcg gggccggctg ctctccccct gacaaacatc
     7621 cccgcgcggc atatattggt ttcatgccgc cgcgagacac ttatccggtg ctcttccgga
     7681 ccgccgccgt gctcctgtcg ctggcgctct ttgtcgcgct cgcggagggc gtcgtccgcc
     7741 tcgctggtct cgatccctat ttccagaacc gcttcttcac tgtcaaccgg gcgctcgatt
     7801 atcccgagat tttcgacaag gaccacgaac tcttctggcg cttccgcccc gatcagagcg
     7861 tgacctcccg cttcttcatc gggcgcaccc accgcatcaa cagcgccggt ctccacggcc
     7921 ccgagatcac gccggccgag gggcgcaccc gtgtcatcac gctgggcaac tcgtgcactt
     7981 tcggatgggg cgtcgcgacc gagcacggct acccccggct gctggattcc ctcctcggcg
     8041 actctttcca ggtcatcaac gccggtatcc ccggctacac ctcgctgcag ggcaagcgtt
     8101 tttaccgatc cgatctgtcc cgccttcgcc ccgccatcgt caccatcctc ttcgctttca
     8161 acgaccactg ggcggccgcc ggcgaccggg ccgacaagga ccagcgtatg cccccgcagt
     8221 ggctgctcga cattcaaaac gcgctcggcc gcctccacac ctatcgcctt ctaaagaaag
     8281 cgatcctctc ggcggtcgaa ccgcccatgg attccctgtt cgaccgccgc acgccgatct
     8341 accgggtcgg gctggaggat ttccgggcga acctcgagga tctctgccgg atgattgtcg
     8401 ccgacagcgc gcggccgatc ctgctcacat cgcccatccc cgacctgccg acctattacc
     8461 cgaaggggta tcagtcgaac ctccacaaat accacgagcg gtacaacgcg gcgatccgcg
     8521 agatggccgc cgcccgcggt tatgatgtgg tggatctggc ggagggcttt gccggccggc
     8581 cggggttgtt cgatgatccc ctggtggatc cggcccactt caacgacttc ggccaccgcc
     8641 tcgcggccgc gatgatcgca gcctgcatca gggacagcct ctagctacgg cgcgctttcc
     8701 gcgaaccggt ccagcaccgc gatcagtttg tcgatgtcgt cttcgttgtt gaagaaatgc
     8761 accgaaatcc ggatcgaacc ctcccggtgc acgcacgtga ttttccgccg gtagagctcc
     8821 ttgtgcaggt cccggaaccg gtcgcaggtg aaagtgaaaa tcgaggaccg gtgtttcggc
     8881 tccatcgacg aggtgatccg gtagaacttg tgtcccctga tgtaatcggc cagccggtcg
     8941 atcagggcgc gggtgtggcg ctggatgttg cgcgtgccca actcgcggaa catcgccgcc
     9001 gattcccgca ttcccgccag attcaactcg acgtagtagc ccagctcgaa ccgcgcggcc
     9061 gtggcgagaa actcccggtc gtaccggaac aggtcgccga acttctgatg ccactccacg
     9121 cccagccagc tggccgaggg gggctggagg cggtcccgga tgcgatcggc gatatagaaa
     9181 aacccgctcc cctgcggcgc gagcagccac ttctggcacc cggtcgagaa gatgtcgatc
     9241 cccagcttcc gcacatccag cggctgcatc cccgttccct ggatcccgtc gaccacgaag
     9301 aacagcccat ggtcccggca gaactctccg agcgcggcga ggtcgttccg gtatccgtcg
     9361 aaaaactgca cccacgagat agcgagcact ttcgaccgct tggtgacggc gcggctgagc
     9421 tgctccacat cgacccgccg gtctcgcgtc ttcacgaacc tcagggtaat cccgcgcgtc
     9481 tgggccgccc cacggaatgc gtagacgagc gccggaaact cgacatcggt cagcagcacc
     9541 tcgtccccct ttttcagcgg cagcccgtag gcggccacgt tcagtccgaa cgtggtgttg
     9601 agcccgatcc caatctggga cggtcgggcg ccgatgagcg cgccgtagtc ccggcggagc
     9661 gcctcccgca tggcgaagat gctcgccgtg ctgtcctccg ccgaccgcac ccgcgccgca
     9721 atcgtctcgg ccagcgccct ctgcacccgg gtcgagtacg gcccgtatga cgccgagttg
     9781 aaatagacca gctcgcgcgt gtgggggaac tcgccgcgaa ccgcccggaa ctgttccgct
     9841 gtcgatgtcg tcatcccgtc ctcacagact gtacaggtag ccgatgatgg ccaggcagcc
     9901 gcccagtttg agcagcaggc cgagcaggaa gtacaataaa tgcggcatcc agagtctcac
     9961 gcgcagcgac aagcccatga tcgcggggac tccgccgagc acgaacccgg ttagggccag
    10021 ggcgagcccc gtgcggatcc ccgccagcgg cagcaccgcc gtgaaaaatg agtacgccag
    10081 agtcgggatg gcgacaaaaa ataacccttc catgacatac ccgaccacga acgccgccgg
    10141 cccccaggcc tcgcgctggg ccggcggaat gcccttggcc atgtccagct tccgtcccag
    10201 ccactcgtgc gccagcgtca cgaccagcag gtacaccccg cccgcaatga gacaataggc
    10261 catcagcgtc atgttgatca cgtgagccat ccttcccggc gataccaggc aaacgtctcc
    10321 cgcgccccgt ccgcaaaacc tatctgggcc tgaaatccga attccgcccg cgctttcgcc
    10381 gtcgagactt cccacgaggc cagtagttcg cggcactttt cccgcgtcaa catcggcgtc
    10441 gccccggcca gccggaacaa tccctccgac acggccgcga tcgcgcgaaa aaccccgccc
    10501 ggcaggcgca ggccgatccc cttgcgcccg cccgccgccg ccaccagatc caccagccgc
    10561 cccatcgcgt aagcctcctc ttcggcgatg aaatacgcct ccccggcgcg cgttcccccc
    10621 gtcagcgcca tcaccaccgc ccggctgaga tcgtcggcgt gcaccagttg cagtttgcgc
    10681 ctgggatctc cgatcaccgg ccgaagcccg cggtgggcgg cggcaaagaa cgtcagcagc
    10741 tcgcggtccc ccggtccgta gacggcggcc ggccgaagcg atatcacgtg aaagcggtcg
    10801 gcgaacgaca agacggcgcg ctctccggcc agcttcgagc gaccgtaggt cgtgatcggc
    10861 tgcggctcgt ccgcttcggt caccgggcgg ccgtcggtcg agggtccggc ggcggcgagc
    10921 gacgaaatgt agaccaattt ccggaccggc gcctgctccg caatcaccgc ctcgcacacc
    10981 cgccgcgccc cggcctcgtt cacctcgaaa taggtcgcgg cgcgtttggc tttgaccgct
    11041 ccggcgttgt gcaccacata gtccgttccg gccaccatgg gccgaagccc ctcgacggtc
    11101 gagagatcgc cgaagcggta ttcaaccggg acatctttca gaagggaaac atcggccgtt
    11161 ttccgaacac cggcgatcac ccggtaaccg gccgcctgca aggcgcgaca cagcctcgag
    11221 cccacaaaac cgttggcccc cgtcaccaga accgttgcct ttgtctcgct caccccgcca
    11281 atgtagacaa attgacaccc gcggacaaca tccgtttggc gaaaggcccg cccggttttc
    11341 tgtcggagtc aaatacgtga tgcccaggat tccataggtg attggtcgga ggttgccgac
    11401 cgctcattct ttgagcgcgg gggagagccc gccgcaaagc accgtatccg gcaccgggga
    11461 gaacgccgcc gcgccgaccg gctgatcgac cggtcctgct tcgccgggcg ctgcgaaccc
    11521 ggccgtggaa cagactaagc gggattccgc gttctcttgc ctcccttcat gcgaaagccg
    11581 ggccgatcac tcggcccggc cgctcgcatt gcactgacct cacagtgggc taatttggtc
    11641 tcacggtgac gcagatgtcg atcccgtggc cgtagggatt cggcttgatc cgctcaatcc
    11701 gaatcgtcgc atcggcggcc agttcccgct ccacgacaaa ctccggctgg ttccagacat
    11761 cccacaggta ctccgcgccc ccgatcgtga tcttcaccac gttcgacggg tgttcgcgtt
    11821 cggccagccc gcggaactcc agccggtacg gccccgcctc gttgctcagc agccagataa
    11881 tctctttctg gccggccccg aggttggcgc agtgcgacgt gaggatcgta tccggcacca
    11941 caacgtatac cgtgtcgaca atcgtcagcg tgtccgtctg gatgatggtg tcagtcacga
    12001 tcaccgtgtc cgtctggatc accgtgtcca cctgaatgat cgtgtcggcc accacgagcg
    12061 tgtccaccag ttccagcgtg tccacggtcg tgaccgtgtc gacatggacg agcgtgtcgg
    12121 tgacaatcac cgtgtcgacc gccagcaggg tgtccgtctg cacgagcgtg tcgaccacca
    12181 ggagggtgtc gtactcggtc agcgtatcga cccaggtcac cgtgtccacc tcgatgaggg
    12241 tgtcgacgat gaaggtggtg tcgtagttgt tgagcgtgtc gacctggata atcgtatcca
    12301 ccgtggcgat cgtgtcgagc acgatcaccg tgtcgtactg aatggtcgtg tcgaccagcg
    12361 tgatcgtgtc ccagaactcg gtcgtgtcga cctgaatgac cgtgtcgtag agcgtggtcg
    12421 tgtcgacgag gaaggtcgta tcataccagt gcacggtgtc gatcagagtc acggtgtcga
    12481 actccagcag ggtgtccacc cggaagagcg tctcgaccgc cgcgctgtcg atcacgatga
    12541 tcgtgtccca gaggaagatc gtgtcgggcg gttccggctc cggccgcggc gggttcccgt
    12601 tgtcgagagt ttccagcggg ctggagcagt tcaggagcgc ctgggcggcc aggaatagga
    12661 gcgccaccgc cgacagaaaa acgactcgtt tcatcgggcg cctcctattt cgacaaaggc
    12721 ggtgagggtc agcatgaact gcccgttctt cgccatcgat ttcaggtcct cggtgatgcg
    12781 gtggcgggcg gggttgtagg cgccggtgat ggcgaggtgg tcgaacggca ccgcccgcac
    12841 cccgaccatc ggcccctccg acagccgcac gtatttgtag tagtactcgg agatctcttc
    12901 aaaccgcatc cagccgccca ccgcgccgac cgggatattt ttcagcggga agaccaccac
    12961 ctgtccgccg gccagccgcc gcttggtttt gagcagttgg tcctcaaact ggaactcgct
    13021 gttccacagc gtgtacccga acaccccttc gaggtacacc gtcctcttcc atgacagcgc
    13081 cccggtgaca atggggaggc cgccgaacgg ggaggtggag acgccggccc cgaactgcag
    13141 accgaggaaa tcgtcaagtg cgggggcctc ccccggcact tctctcaccg tctcggtgaa
    13201 gtgcttttcc acgggcggcc gctcctcgag cgcgctcacg cggcccccga gatccgacag
    13261 ctcccgcacg acccgcacgc tcgcgaagcg gtgcgcctcg ccggccgcga ggacgtcgac
    13321 cgactggatg atgatccggt gcgggtcgat cttgaatttg tccaccagca ggttgcgcag
    13381 gacatgggcg cgcccgatcg ccaggccggg attcttcgcg tcgtggtgct cgcggtactc
    13441 cagcccgtcc gccccgcccg tcaccaccgc cagcatcagc gggtactgcg ccagcgtatc
    13501 ggccagcgcc tgcaggtcgg cgtagtacgg ctggaaaaac tcctcgccga tgctgccgcc
    13561 gaccgcctcc gggaagcccg ggatgacgac ttcctgcgcc ccggctgtcg cgccgaggac
    13621 gagcccaagc gcgaccatcg ttaggaaagc tctcttcata gaagacctct gtttacgtcg
    13681 ggagcgccgc ctgtcggccg tggtcagcgg ctgcacagtt ttcggcccgc gccgccggcc
    13741 cccggtccgg gaccggcccg cccggccgtc tattccgcaa tgcgctgcag gttgcatgct
    13801 cccggtttgg tcattgtttc ttctcgccat tggcggcggt cggcggaaga ctgctggcct
    13861 tgcccgattc gccgcttctg acgtagcctg acagcaaacg ccgttcctgc cgcccggcac
    13921 ctcccgcttg tcccgccggc cccagtcggt tatactgtac ccggactctg tgagaacgac
    13981 cgacagaccg gtaaccgagg agagaggccc gaccatgacc aacgagaaat caatctctca
    14041 gaagatggcc gagttcgccg tcgaactccg ctatgacgac ctccccgaag acgtcgtcca
    14101 tgaagtaaaa cgctacctct acgattcggt cggctgcgcc tacggcagct accataccaa
    14161 ggacgtcaac atcatccgcg acctctgtcg cgagatgggg ggccgcccgg aggccacggt
    14221 catcggcttc ggcgaccgcc tccccgcggt caacgccacc ctcgtcaatt cgctcatgat
    14281 ccgggcgctc gatttcaacg acatctactg gcgggaggac ccttcccacc cctccgacct
    14341 gatccccgcc gcgctgtcgg tcggcgagct ggtcgatgcc cccatgcgcg acgtcatcgt
    14401 ggccatcgtc ctcgcctacg agttcgagca gcgcctctgc gaattcgccg tccccggcgt
    14461 ccgcgaacgc aagtggcacc atgccaccct cacccaattc gtctcgccga tcgtcgcggg
    14521 caaagtgctc gggctgacag ccgcgcagat ggccaacgcc atcggcatca acggctgcca
    14581 caaccacacc atcggctgcc cgaccgccgg caagctgacc atgatgaaga acacggtcga
    14641 cccgatggcc gtgcaggcgg gcgtcttcgc cgccctcatg gccgggaagg ggtacaccgg
    14701 cacgaccgcc gtgttcgacg gcaaagaggg actcatggat gtgttcggcc ccgagtggga
    14761 cctccaccgc ctcctcgggc acctcggcga gaagtacaaa atcctcgagt gcggcatgaa
    14821 ggcgtttccc accgaagcgc tcacccacac ccacctgacc tgcctgctca aagtagtcac
    14881 cgagaacaac atcacctacg accaggtgga ggaggtcata gtcaccacca tcgcccgcgc
    14941 ctgcgacatc ctcttcgacc cccacaagta ccgtcccgac tcgcgcgaga cggccgacca
    15001 ctccctcccc tactgcctcg ccgccgccct cgtcgatcac gaaatctccg tccagtcgtt
    15061 cagcgagaag aagctcaagg acgaacgcat ctggcaggtc atcgacaaaa tcaagggcga
    15121 ggcgtcgacc gagttcgagg ccatgttccc ggccaaacag ccctcgcgcg tggtcgtgcg
    15181 gaccagggac ggccggtcgt actccgccta cctcgagtac cccaaggggg atccgcggga
    15241 accgatgacc atgcacgacc tcgaggtgaa gtttaagggg aacagcgcgg ccctgctggc
    15301 tcccgagcgg cagacggcgg tccgcgacgc catcttcgcc tgcgagaaat tgtcctgccg
    15361 ggacctgatg gccaaactcg tggtgtgaga ccgcggcccg gggtgggccg ccgcaaaggt
    15421 caaaactcct gcggtattga cggcgagcca tgatagccga ccaccgacat tcgcttcggc
    15481 gtccgcgcgg cgtgttcgac tttgccgcac ttgccgaacg cccggtcccg gcgattttcg
    15541 ctcaccgggg ggcctcgcgc ctccaccgcg agaacaccct ccgggcattc accgcggcga
    15601 tcgccctggg ggccgatggc atcgaggtcg acgtgcgccg cacgcgcgac ggcgtcctgg
    15661 tcgtgcacca caacgcccgc cccctcggat catcgcgccg gatcgcgagc ctcgactacg
    15721 acgccctcct tgagctggcc cgtcgccgcg cctaccatgt ccccacgctc gaggagacgc
    15781 tcgacctgtg cgcggggcgg gccgccctcg acctcgaact gaaagagtct ggttatgaag
    15841 cggaggtggt ccggctggtg cgccgccgct acgatttgcg ccacgtcgct ttcacctcgt
    15901 tccgcggccc ggtggtcgcg gcccttcgga aagccgctcc ccgcgccgcc ctcggcctgc
    15961 tggtcggccc ggcggcgccc ggcacgttgc gcctggccct ccggcgcagc gctctggcgc
    16021 ggaaaataga ccgcgtcggc gccgacttca tcgcccccca ccgccgcctc gtcacccgcg
    16081 ctttcagcgc ccgcatggag gaactcggcg tgcctgtcgc cgtctggacg gtcaacaccc
    16141 tccccgccgc gcagcggctc acgcgccgcg gtgtggcgat cctcatcacc gatcttcccg
    16201 gcaccatcgg gccggcggtg cgaagcctcg gggagaaagg cgcgcgaggc gagccgccgc
    16261 gcgaggattt gtgatgaaac cgcactacct gctgatcctg ctcgccctgg ccatgctcgc
    16321 cggcggagct gtcggcctcg cccgcaccgg gtcgcccatc ccccttctca tcaacagcgc
    16381 cctggccctg ctcaccctta tcctggcgct ggtcgtttcc cgggactcgc gccgggtact
    16441 gctcgccgct accgtctggg tcgcgctgtg cgtcctcctt tacacctata tgacgatcat
    16501 tccgccagcc gcccacaacc cttcgcgtcc cggctcaaag tacctcttcg ggtcaatggc
    16561 tctggtcgcc ttgatcgtcc tggtcggcct gagcgggggg cttcgccgtc gccgatctca
    16621 acgccgacta tgagagctat ctcagtgatc gccgattcaa gtcgttattg cataagttct
    16681 taatatgcaa tatagggcac cggcaaaaaa accggcacaa actatcagga aatgtggtat
    16741 atatgactaa caggttagtt aaatcaactg gttagttatg gatagagaag aaactgcatt
    16801 atcccccggc cctcctgagc cggcggattc gacctccatg cggaatcgta ttctggaagc
    16861 tgctttaatg gagtttgcgg agcgtggcgt ggccggggcc cgtgtcgata ggatcgcggc
    16921 ccgtgccggc gtcaacaagg cccttatcta ctactactac acttccaagc agcgtctgta
    16981 tgaggagtcc atttcaagtt acatgcgggc tctcctcgac agtgtgcggc tcagcctgga
    17041 cgaggcgtcc aacctcgagg aggccttgca cggagtggcc gagcgctact cctcgatctt
    17101 tctgacgcgc ccggaaattc cgcggctaat gatgcgcgag ctggccaatc ccggcaacac
    17161 gctggtgtct cagtgggcgg agcgtatcac cgcctcgggc gttccggcga ccatccggcg
    17221 gcgcttggag gccgggcgcg aggcggggga ggtccggccc atcgacttgc ggcaggcgtt
    17281 cgtctcgttc ctcacgatgc agatcggcta ctttctcatg tcgccgctgg tcgaccgcgt
    17341 gctccacctt gaggaccgcg aggcgttcgt cgtcgcccgc cgggacgcgg tcgtggacct
    17401 gttcctgaac ggagtgaaag cacgatgagg cgcactttgc ttgcgggcct tctggctgtg
    17461 ctggcggccg gaccgtcgcc ggcggcccgc caattgaccc tgcgcgaagc gctgacgctg
    17521 gcgcgggagc attcattcga gatccgacgg gcgcttgccg aggagcaagc ggccggggag
    17581 gcgctgggcg ccgcccgcgc ggcccgtctc ccgacccttg acgcgtcggc gatcgccttc
    17641 tacaacaccg aggttgcttc cctcgacctc gctacctcgc tgggacggct gtcgcgggag
    17701 atcggcacaa aagagaatgt ccagaccgat ctccgcctgg cgttcccgct gtacaccggc
    17761 ggccgcatcg ccggcggcgt cgacgcggcg caggcctcgg cccgccggca ggcggcgctc
    17821 accgaggcgg cggtcgacga cctgctctac cggacgcggg tcgcttacct gaccgtgcac
    17881 cggagcgacc gtcagatcga agcggcccgg tcggcgctcc ggcgggccca gatcacccgc
    17941 gcccaggtgg cctcgttgct ctcggccggg gcggccgatt cggtggctgt gctcgaggcg
    18001 cagctggcgg tgagcgagac cgagctgcgt ctgcagcagg cgctgcacga ccgccgggag
    18061 gccgtcatcc aattcgccca gttggtcggc ctgtcggcaa gcgactcact cgatctagct
    18121 actccgcttg cgccccccgc cgcccccggc gatcccgaca ccgggtttgt ccgccccgaa
    18181 ctggccgccg cgcaggcggc ggttgagatc ggccgcgcgg gcgtcgcccg ggcggcggcg
    18241 gactggttcc ccaccctggc ggctttcggc ggctactcct atggcaagcc gaacctcgac
    18301 cggttcggca acacctggaa cgactactgg caggtcggcg cgcggctgga ttggtcgttc
    18361 aacctcgggc ggggcacgcg gcgcgaagtc aaccgcgcga aattcaccgt cagcgccgcc
    18421 gaaaaccaac ggaaccgcct cgccgaacag ctccgcgagc agcacctgct cgcctgggag
    18481 cgcctccggc tggcgtacga gcagtatctg accgcgctcg accgcgccgc cctcacccgc
    18541 cgcaactatg aactggcaac gagccggcac agcgagggag tgctgtcgtc caatcgcctg
    18601 atggaaatcg aagccaccct gaacgaggcc gaggcgggtc tcgccgccgc cgaggcggga
    18661 tactacatcg cgcgctcggc ctacctgtac gcctccggcg ccgacctgat gacggaagga
    18721 ttttgatatg agattgctgc ttttcactac tgcgctgttg ggactgctgt tggcggccgg
    18781 ctgccgcaac gaggatgcgg cgctgtccgg ctcgggcatg atcgaagcga ctgaggtcac
    18841 ggtctcagcc caggtcgggg gccaggtcga gaagctgtat ttcgacgagg ggacggttat
    18901 cgcccccggc gacactctcc ttgttgtcga caccaccgct ctggggctcg aactggcttc
    18961 ggcgcgcgcc ggccgcgcgc tcgcccggac ccgcctccag gccgcgcagc tccggctgaa
    19021 cgacgctctc gtgaccgagg cctaccttgc caacgaggtg aaacgcatcg agacgctcgt
    19081 ggccgccaac accgccaccc ggcgccagct cgacgagctt cagtaccgcc acacccaggc
    19141 cggaattgcg gtcggcagcg cccgctcggc gatcgcctcg gccgaggcgg aaatcgccaa
    19201 gatcgacgcc gacatcgccc gccttttgga ccaatgggac gatgcttttc cggtcgcccc
    19261 gatcgccggg acggtgacgg agaagtatgt cgatgcgggg gaactgctcg cccccggacg
    19321 gccgatcgcg cgcatcgccc ggctcgacac cgtgtgggtc aagatctatc tgccggcgcg
    19381 ggagttcgcc gccgtgacta tcggcgccgc cgccaccgtc gataccgaag cgggggaatc
    19441 ctctcccggc cgggtcacgt gggtggccga ccaggcggag ttcacgccca agaatgtgca
    19501 gaccaaaaag gcccgggccg atctggtgta cgccgttgag atcacggtcg ccaacccggc
    19561 ccacaaactg aaaatcggcc agccggtgtt tgtgacggtg ggggagtgat ggctttcctc
    19621 gaggtccagg gtctggcccg gtcgtaccgc ggcgtgcggg cggtcgagga cttctccttc
    19681 gccatggagc gcggcgagat ctacgccctt gtcgggccgg acggcgccgg caagacgacc
    19741 gtcatccgcg ccatcgccaa tctcatcacg cccgaccgcg gccgcatcct gcttgacggg
    19801 accgacatcc gcgaccggtt cgatcgggtc aaatcccgcc tcgggtacat gccccaggta
    19861 ttttcgctct accccgacct gagcgtggag gagaacctca ttttctacgc gggggtctac
    19921 ggcgtgaccg gccgggcgta tgaagagcgg cgcgaccgga tgtacgattt ttcgaacctc
    19981 cgccccttcg ccggccgccg ggcggcggcc ctctccggcg gcatgaagca gaaactggcg
    20041 ctctcctgcg ccctcatgca cgaccccgag ctgttgctgc tcgatgagcc caccaccggc
    20101 gtcgacccgc tctcccgccg ccagttctgg gacattcttc tgcagctcaa gcgggacggg
    20161 gccgccgtgc tcgtcaccac cccctatatg gatgaagtgg cccgcgccga ccgcgggagt
    20221 ttcgtcttcg gcgggcggaa actcgccgag gggtcgccgc gcgagttgac cgagtcgttt
    20281 tcgggcgagg tctatttcgt cggcgcggcc gccgacctcg agcttgtccg ccggctcaac
    20341 gagctgcccg aggtgaccgc ccagcgcttc ggcgc
//
LOCUS       DAOPOE010000057        20189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3288_length_20189_cov_5.650641, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000057 DAOPOE010000000
VERSION     DAOPOE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_20189_cov_5.650641"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKM94_08715"
     CDS             complement(<1..257)
                     /locus_tag="PKM94_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66758.1"
                     /translation="MKADFRINGTAVGAIARRNFLSYFASPTGYVFITLFIFLSAAAA
                     FWQERFFADNLANLDQLNQFFPYLLLFFVPALTMGIWAEER"
     gene            complement(247..1038)
                     /locus_tag="PKM94_08720"
     CDS             complement(247..1038)
                     /locus_tag="PKM94_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66759.1"
                     /translation="MTDTAHSAVIEARGLSKYFGSFVAIEDLSFAIPRGQIVAFLGPN
                     GAGKSTTMKILTGFMAPSKGRATIAGFDVGTDRLEVARRLGYLPENGPLYDNMTPLEM
                     LRFLGEARGLAPQRLADRIEAVNQICALHLVLEKPIGKLSRGYRQRVGLAQALLHEPE
                     VLIMDEPTAGLDPNQIRDFRTNIRRLAEGTTILLSTHILQEVEAVAQRVLLVHNGRLK
                     FDGTPAELAAVGSMEKKFHELTRSNGANAAAGGPAAAEGGGAYEG"
     gene            complement(1258..3282)
                     /locus_tag="PKM94_08725"
     CDS             complement(1258..3282)
                     /locus_tag="PKM94_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOD66760.1"
                     /translation="MKHKMVLKTWALAGAAFLLVPGAQADVSKAVVWDIPTDVAVKQF
                     GALPGSALTADEIRSISQYRYTEDTLKVLVICVEWWDRPHTYSRETLDSMLLSRDVYG
                     GGSLADYFDEVSYGQGVVVGQVLEWYNAGYYMGNVQYNFEALLAELDPVVDFSQFDGN
                     GDGDVDAVIFLRSGTGQEDSRESEDIWSYAMSYATGHGPGPFDGVHVSHWNTSPELRP
                     LRDPAWPPYFSGLDSLNNIRVFCHELAHNFGLPDLYDYDAKTDTTTYFTPADSNDHPV
                     YDWCLMGYGGYGIFSIKSRVPSHLCGWSKMQIGWLDPIEVTGTCENLVLYDVETHTDS
                     SLYRLPIRPWEGEYFLLEFRNPRAGGKFDKLDSDFSVYFPNTLAYGCDTLDGGLIITH
                     VHDSLGAWWFRINYGWPWYPHYTVAVEDAGYNPAINMSANPEGYVTDSAQWWYPYETR
                     KGAAFSPNVPGQNLFSPTTYPSSDGYEGPSGIIVRVDSIVGDRMYLYVHNPFFYDADG
                     DGAADGADNCMDTYNPDQANADGDQFGDVCDNCPTVTNADQADYDEDGIGDACDACPA
                     DPFNDADGDTLCAEGDNCPNAYNPGQEDANEDGIGDACCCLLRGDMNLDGAVKVSDLT
                     MLINFLFRGGPASGCPEHADVNGDGGVAVSDVTALITYLFRGGAAPPSCR"
     gene            complement(3896..6733)
                     /locus_tag="PKM94_08730"
     CDS             complement(3896..6733)
                     /locus_tag="PKM94_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD66761.1"
                     /translation="MICAILCGIAPAAGAADLYRVAVESAADARALNAAGVDAAVRVR
                     GGYLVLVDARALPALAESGLRYDLIATTVDRSTLALGTHHDGANPGGYPTVYAEDGVR
                     LYRVEPGDVRDRAEADGLASLPPGSRRIVYREPAPREVKQRAPGSLDLETLIALVRQD
                     SLQSYTLQLQAFPNRVTGSLGNRQSRDWLQNRLISYGLDSAYQDSFLYGSTNCQNVIG
                     VKMGTTLPDHRIVVGAHRDAVSGLPGADDNGSGTAAVLEMARVMADIPTEMTWVFALF
                     DSEEQGLNGSYHYAASAAANGDSIIMMLNMDMIGYNGNVNNVSVHYGEDTVWASRWKI
                     IADSLSTNLIGYFSGSASNSDHYPFQQEGYDVVFLIEYNFSTVYHSEQDSTTYMSFPY
                     MRRIVQASLATAYDADGAYLPTPGVLFTEVDPIPFMVPPVDTSEFLISITPGPGGAIA
                     PGSAQVHFRYDAGAWQTGPLADLGGGTYAVPLPGPLCGERVQFYVSAAEVTTGMYTYP
                     DSASPWSAAAATQFTTALADNFETAQGWTVTGNATAGQWARGVPAGGGSRGDPPTDFD
                     GSGQCYLTGNSAGDSDVDGGTTYLTSPTFDATGGDDALVHYARWYSNDFGASPYEDYM
                     DVHVSNNNGSTWTLVERVGPSGTQVSGGWFEHSFEIGDFTAPTNQMRLRFTVGDLGSG
                     SVVEAAVDAVSVTAYTCEEGYFVITTEDLPDWTAGVPYSVQLEAGGGVGARTWADKFG
                     DLLGTGLSLSSGGLVSGTPTASGTIAFTATCTDEADSTVEKAFSFLMNAPVSIATRSL
                     PNGVVGEAYAQTLTAVGGTGGRTWSEPGGGLAGTGLTLAANGLLSGTPTAAGPIGFTA
                     RVEDAVGGFDEESFSFVIELPYVCGDVDNSGEAPNVADLTFLVNHLFRGGPMPPVAEA
                     ADVNGDGNLTVADLTRLIDFLFRAGGPLECE"
     gene            complement(6835..10383)
                     /locus_tag="PKM94_08735"
     CDS             complement(6835..10383)
                     /locus_tag="PKM94_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOD66762.1"
                     /translation="MKALVLTVTLCLLVAGAVFAANDEEGPAGSNRLRLDPNVTRYLE
                     SEDGVPLYVSGTFAQKVARGNEAASAYSFFEEYKASYRMTDPRAELAVERLVTDDLGM
                     RHLRMTQRYRGLPVIGGDLVVHFTAQDQLRGVNGTFVPGISLEVTPQLAAAEAVRLAE
                     TDLASFFGQGTPAEPQLVVFPWEGTTHLAWRLFLYSDTPMGRWEYFIDARSGAVIYKA
                     NRIMNSDAIGTGIGVMGAARDHIDVDYTGSTYQMKDYTRQAANNPHGHDGQMPAGNYI
                     QTNIAGSTLPGSIATDADNYWDVAATQRPAVDGQVYTALMYDYLLAHFGRNGYNNAGA
                     SMLTIVNYSGDGNDNAYWDGSRIVVWSWSTGWRSLAGCPDVIAHEWGHAVTEYESNLV
                     YQKEPGALNESFSDMIGAAFEFYYDTLDVPDWDMGENGRTTGVAFRSMDNPHEFGDPD
                     TYGTGDPYWVDVVNCTPSSWNDYCGVHTNSGVGNKWFFLLSDGGTHNGVTVTGIGVHN
                     AIRVAYRANAYYWNSQTTYHEAAIATLDAADDLDPSGLWSQRAARAWTAVNVAVPLPG
                     LAFSFPDGVPSTITPTDSTGFPVVIAGVYGGEMMTGSQRLHYRTDGGAWEHQVMAPAA
                     GDTFTAMLPPAECGSTVEFYFSARLSDGTTYEEASMANPLSAIPMTEVTEVMDDNFQT
                     NLGWTVTSTATAGQWQRGTPVGGGDRGDPPTDYDGSGQCYLTGNTDGDSDIDGGTTTL
                     ISPTFDLSGGDAEVSYARWFSNTYGSAPNEDVMEVFASNDNGNSWVLVETVGPVDEAG
                     GGWYTHSFLVGNFLTPSAQMKVRFVAGDLGSGSVVEAGVDAFKVTRFTCNSGIPAPAI
                     QTTDVPDWTIGVACSQQLAATGGTGALTWSDLNGDLVGTGLSLTTDGLLTGTPSAAGP
                     ITFTALVTDENAQTDQQLLNFTINSAVVVNAATLPDWTAGVAYAQQLTASGGTGARTF
                     SDKLNSLAGTGLTLNAAGALTGTPAAAGSIGFTALATDAVGATGERVFGFTVNPAVSI
                     TTASLPDGKAGEAYTHAMEGTGGTGALVWSDAGGTLAGLGLAISDAGLITGAPVDSGT
                     FTLTLQAADQVGSTDQVELSLHVAPAFICGDIDGDGTIGVADVTYLVAFMFRGGEAPP
                     IMAAADLNATGAIEVNDLTMLISYLFRSGPLPTCAP"
     gene            complement(10588..11430)
                     /locus_tag="PKM94_08740"
     CDS             complement(10588..11430)
                     /locus_tag="PKM94_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOD66763.1"
                     /translation="MLDFFHAVTQYTFVLNALLTGILASVACGMVGSFVVARRITYIA
                     GGIAHSVLGGMGVARWLELVHGWPVKPLYGAVAAALAAALTIGVVSLKAREREDTVIG
                     AIWAIGMAVGILFIAATPGYNEDLMSYLFGNILMVGPVDLWLIGGLDLGVMAVTLLFF
                     NQLQAVCFDEEFARVRGLNVEAYYLLLLGLTALTVVILVTVVGIVLVIALLTLPVAVA
                     GYFARTLGRIMAASIGLSILFTVAGLAASFGPNLPAGAMIIMAAGGSYLAVVAATRLR
                     RAIR"
     gene            complement(11430..12188)
                     /locus_tag="PKM94_08745"
     CDS             complement(11430..12188)
                     /locus_tag="PKM94_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66764.1"
                     /translation="MAERAAVVLEQVTFGYDSEPAVEEASFAIGEREYVWIVGPNGGG
                     KTTLVKLILGLLQPRRGTVRVFGAPPEEARHRIGYMPQHARLDPKFPVTVFEVALMGR
                     LGPRRWIGRYRRADRKAALRALDQVGLADMRNRNLAELSGGQQRRLLIARALAAEPEL
                     LLLDEPMANLDIRGENELTRLLQELNNRLTIIMVSHDPAFVAGSVKRVVCVKRRVHTH
                     PTATLDSGFMRELYGTEFRVVRHDVEQGSGEGAA"
     gene            complement(12191..13126)
                     /locus_tag="PKM94_08750"
     CDS             complement(12191..13126)
                     /locus_tag="PKM94_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOD66765.1"
                     /translation="MIENRLIKWTGRGIGAVGAAVAIGLAPAGCGSGAKAPGGTIQAF
                     VTVAPQAYFVERVGGDCVAVDVLVPPGQSPHTYEPTPRQMARLEAADVYFRAGMPIEN
                     RWIGRLAEIVGADRIIDTRAGVALRSIEGHSHEEGDEEEAAHGKTPDPHIWLDPVAAK
                     TQARTIAEALGRLDPGRREDYGARLAAFEHDLDSVHALIGEILAPCAGRAVYVFHPSY
                     GYFLDRYGLRQTAIEREGKEPAARDLADLIDAARADSVRALFVQPQFARKTAAAVAHA
                     VGAELVTIDPLARDYLPNLVDMARRIAAGTAPEVK"
     gene            complement(13169..13516)
                     /locus_tag="PKM94_08755"
     CDS             complement(13169..13516)
                     /locus_tag="PKM94_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOD66766.1"
                     /translation="MRKKSEIVTFKVDGALLEELRKVPNRSAFIRTAVLSALRATCPL
                     CQGTGILSASQQRHWDRFAHDHAVTECDDCHELYLVCEHEAGQRPGRKPAGRKPAAGA
                     VKAGRRGRTRRGK"
     gene            13938..15701
                     /locus_tag="PKM94_08760"
     CDS             13938..15701
                     /locus_tag="PKM94_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66767.1"
                     /translation="MKRPRFVSKRGVGRRTVQRDRLVFRAMIVAASLVAVLLVAADGW
                     SACTQPPPKMGLWLPFDEASGTAAHNVRGLDGALVGGPSWITGKVDNALFFDGGNQHV
                     AVPHYWTNPFSAARGRGIDIRENDFSIDAWIQREGLAGIRTIIDKRQDIGGYLSGYSF
                     FLLDGRLALQLACGAAGYNNWAVPAGNAIPVSGTWHHVAVTVDRDSPTGLVFYLDGAP
                     VTTLNPSGYQGTLHNTSELWVGDRIVSPQGGACFAGGIDELEIFPRVLSAAEIQKIYA
                     AGADGKCKVTLGAEVYDGPSPTGWAKLTGLIINSGIEPLTDDYSFAGVPDTFPIEFTP
                     ASGSVTVPAREVTMIGSEAIVDTQDIQHCWPYQMFFNGFAPDGDTLCIDSMWCAGFEK
                     GFFAAVTDCVDNLGSLKIFNAGFPDTFPIDFRIAAAIDYSYQGDTLMIDSMGYAPCVS
                     LNGLNPGQPVYGNANVLPGDSVTITLTAKFVDYALLDPYMILLQVYRSSQWQTLASMY
                     FVNVLTPPSCCVARGDLTGDGSLKVSDLTFLVNFLFRGGPSPNCPDHGDVNNDGGIKV
                     SDLTYLVNFLFRSGPPPPPCD"
     gene            16100..16990
                     /locus_tag="PKM94_08765"
     CDS             16100..16990
                     /locus_tag="PKM94_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66768.1"
                     /translation="MISVEHLHKRFGDIAAVSDVSFDIAAGETFGLLGPNGAGKTTTI
                     SMMVGVLAPDSGRVRLDGIDDPTRPALRRKIGNAPQALALYGDLTAAENLAFFGRLYG
                     LSRARLAARVAWALELAGLTDRRSHLVKTFSGGMMRRLNLACAVVHDPPLLLLDEPTV
                     GVDPQSRNQIFDTIEALGREGRTILYTTHYMEEAQRLCDRVAILDHGRILALDTVDRL
                     IHKHGGKSVIEAELERPPDPGQPLPGDLTGTSLRLETERPLEEIAALADAGVRFLRLE
                     IHRADLETVFLNLTGRRLRN"
     gene            16990..18255
                     /locus_tag="PKM94_08770"
     CDS             16990..18255
                     /locus_tag="PKM94_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66769.1"
                     /translation="MKTLLTLARKDLRLLWRDRFGLFWVIVFPLLMALFFGSIFSTGG
                     ARAGSMKIAAVDELRTEQSRQMIGKLSAKPVLAVTPLPLDSARLLVQKGKLTAYVLLK
                     RDSLATGEGNPFAMPPIEIGLDPSRKAEAGYLQGMVTEAWFSLMQDRLADPAVAKTWV
                     NEGLAGLRADTALPPDQRGVLTRLLASFDDYLTTFDSAEVSQSSPFGKTAITVVEVAV
                     EREGPRSPFEITFPQALTWALLGCALTFAQSIVSERQRGTYLRLRLAPITRGQILAGK
                     GLACFVTSGAVSVALLAVGVLIFGVRVAFPLGMAIALAAAGCCFVGLMMLISVLGRTE
                     QAVSGAGWAIMLVMAMAGGSMVPLMVMPSWMLTVSNFSVVKWAIYAYEGAIWRGFTLG
                     QMLVPAGILTAIGAIAFALGVAILARTDR"
     gene            complement(18329..18949)
                     /locus_tag="PKM94_08775"
     CDS             complement(18329..18949)
                     /locus_tag="PKM94_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66770.1"
                     /translation="MHRKLTVVLTLCGMLLAPPALMAEKTAAGKVSKNPFDHRAVGGG
                     SISIDKIDGLVADGQIQAGKKITFHLRFTNKTGLPVAGFANGFRVYSPDGATWGTTVA
                     DTTGLMPKGLMDGGMFITTFGISGSGADTVGFGGFRILKDGLPDGWSDVPATITIGPI
                     PASMVGKTVCLDQSFFPPGGYWLWSLEDQVENEPDFGGPYCFKIVK"
     gene            complement(19496..>20189)
                     /locus_tag="PKM94_08780"
     CDS             complement(19496..>20189)
                     /locus_tag="PKM94_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66771.1"
                     /translation="AGVIAVTTDGVHWERPVPPAGEQLQLNSVAASGSRFVAVGDQGR
                     IIWSEDPAALWQVAADVPVTNPLHGVTWANGLFVAVGTASVVLTSTDGNVWTADTVDA
                     ATPLNLTAVAFGNGQFVAASSNGRILTSTTGSAWNVFEARPGDTAVYAINNLAFSGAR
                     WVAAGSRNAGAAAEDVIVTLYSADAVTWSVGAAGFAGELHGVAWGAGRFVTVGEIDIG
                     TAENVALILSSP"
BASE COUNT         3359 a         7134 c         6213 g         3483 t
ORIGIN      
        1 tgccgctcct ccgcccagat ccccatggtg agcgccggga cgaagaacag gaggaggtag
       61 gggaagaact ggttgagctg gtcgagattg gccaggttgt cggcgaagaa ccgttcctgc
      121 cagaaggcgg cggccgccga gaggaaaatg aacagggtga tgaacacgta gccggtgggg
      181 ctggcgaaat aggagaggaa attgcgccgg gcgatcgcgc cgaccgccgt cccgttgatc
      241 cggaaatcag ccttcatacg cgccgcctcc ctctgccgcc gcggggccgc ccgccgccgc
      301 gttcgccccg ttggatcggg tcagctcgtg gaacttcttc tccatcgagc cgaccgcggc
      361 gagctcggcc ggcgtcccgt cgaatttcag ccgtccgttg tgcaccagga gcacccgctg
      421 ggcgaccgcc tcgacctcct gcaggatgtg ggtcgacagg agaatcgtcg tgccctccgc
      481 cagccggcgg atgtttgtcc ggaaatcgcg gatctggttc gggtccagcc cggccgtcgg
      541 ctcgtccata atcagcacct ccggctcgtg gagcagcgcc tgcgccagcc ccacccgctg
      601 gcggtacccc cgcgacagct tcccgatcgg tttctccagc accaggtgga gagcgcagat
      661 ctggttaacc gcctcgatgc ggtccgccag gcgctgcggc gccaggcccc gcgcctcgcc
      721 gagaaacctc agcatctcca acggcgtcat gttgtcgtac agcggaccgt tctccggaag
      781 gtagccgagc cgccgcgcca cctccagacg gtccgtgccc acgtcgaatc ccgcgattgt
      841 cgcccttccc ttgctcgggg ccatgaagcc agtcaggatc ttcatggtcg tcgacttccc
      901 cgccccgttg ggaccgagga aagccacgat ctggccccgg gggatggcga acgacagatc
      961 ttcgatcgcc acaaacgacc caaaatactt ggagagaccg cgggcttcga tcacggcgga
     1021 atgcgctgtg tcagtcattg tgtctccctc atgtttctgc ggtcagagcg cgaccgcgcg
     1081 atggtcgctt ctctccattt tcccgtctcg ggaaatggct acaaaaatat ggctcgcatc
     1141 gccgtctgtc aagacaccgg cgccccgcga gccaccagat tattgtaaag acaacgcgcg
     1201 gcgcagttcc cttcgaaacc gcgccgcgcg tcgcggcagt ctgcgcccga cccgcgccta
     1261 ccggcagctc ggcggcgccg cgcctccccg gaacagatag gtgatcagcg ccgtcacatc
     1321 cgaaacggcc actccgccgt ccccgttcac gtcggcatgc tccgggcagc ccgaggcggg
     1381 accaccgcgg aacaggaagt tgatgagcat cgtcagatcc gacaccttca ccgccccgtc
     1441 aagattcata tccccccgca gcaggcagca acaggcgtcg ccgatcccgt cctcgttggc
     1501 atcctcctgg ccggggttgt aggcgttcgg gcagttgtcc ccctccgcac acagcgtgtc
     1561 gccgtcggca tcgttaaacg gatcggccgg acaggcgtcg cacgcatccc cgatcccgtc
     1621 ttcgtcatag tccgcctggt cggcattggt gaccgtcggg caattgtcgc agacgtcgcc
     1681 gaactggtcc ccgtccgcgt tggcctggtc gggattgtag gtgtccatgc agttgtcggc
     1741 gccgtcggcc gcaccgtccc cgtcggcatc gtagaagaac gggttgtgca cgtagaggta
     1801 catacggtcg ccgacaatgg aatcgacccg cacgatgatc cccgacggtc cttcgtagcc
     1861 gtcgctcgag ggatacgtcg tcggactgaa cagattctgc cccggcacgt tcggggagaa
     1921 ggccgccccc ttgcgcgtct cgtagggata ccaccactgg gcgctgtcgg tgacgtagcc
     1981 ctcggggtta gcggacatgt taatggcggg attgtacccg gcatcctcga ccgccaccgt
     2041 gtagtgggga taccagggcc agccgtagtt gatgcggaac caccaggccc ccagcgaatc
     2101 atgcacgtgc gtgatgatca gccccccgtc gagcgtgtcg catccatagg cgagggtgtt
     2161 ggggaaatag acgctgaaat ccgaatcaag tttgtcgaat tttccccccg cacgggggtt
     2221 tcggaactcc aacaggaaat actccccctc ccacggccgg atcggcagcc ggtacaggga
     2281 actgtcggtg tgtgtctcga catcgtagag gaccaggttt tcgcaggtcc cggtcacttc
     2341 gatcgggtct agccacccga tctgcatctt gctccacccg cacaggtgcg agggaacgcg
     2401 gctcttgatc gagaagatcc cgtacccgcc gtaccccatc aggcaccagt cgtagaccgg
     2461 gtggtcgttg ctgtcggccg gggtgaagta cgtggtcgtg tcggtcttgg cgtcatagtc
     2521 gtagaggtcc ggcaggccga aattgtgggc cagctcgtgg cagaagaccc ggatgttgtt
     2581 gaggctgtcg agcccggaga agtagggggg ccaggcggga tcgcgcagcg gccttagctc
     2641 cggcgaggtg ttccagtggc tcacgtgaac gccgtcaaac ggccccgggc cgtgcccggt
     2701 cgcgtacgac atcgcgtacg accagatgtc ctccgactcc cggctgtcct cctgcccggt
     2761 gcccgagcgc aggaaaatga ccgcatccac gtccccgtcc ccgttcccgt caaactgcga
     2821 gaaatccacc accgggtcga gttccgccag gagcgcctcg aaattgtact gcacgtttcc
     2881 catgtaatag ccggcgttgt accactccag cacctgtccc accactaccc cctggccgta
     2941 ggagacctca tcgaagtagt ccgccagcga gccgcccccg tacacgtcgc gcgagagcag
     3001 catgctgtcg agcgtttccc gcgagtaggt gtgcggccgg tcccaccact ccacgcagat
     3061 cacgagcacc ttgagcgtat cctccgtgta gcgatactgc gaaatcgacc ggatctcgtc
     3121 ggccgtcagg gccgaaccgg gcagagcgcc gaactgtttg acggcgacat cggtcggaat
     3181 gtcccacacc accgctttgg atacgtccgc ctgcgccccc gggacgagca ggaaagccgc
     3241 gcccgcaagc gcccatgtct tcagtaccat cttgtgcttc ataggattcc tctcaccgga
     3301 cccgagatga cagattcgtc cattctacct taccatacgg acagccgggt cggtctcccc
     3361 gccgatttcc ggccggcgca aaaagaacgg ggaccggcgt aggagaaccg gtccccatct
     3421 ctgatgggtg tatggtagca aaaggcgacc cggcagtgag taggcagcta ccgggtctta
     3481 actcatcacc agccgggcgg ctgcatgaag cccccagtca ctccccactt gcaagtcctc
     3541 acaagtccct gaccgaaatt gtcgtccccc tcatgacccg acgtcttgac gactcttcga
     3601 tctacccctg tcaacaatcc cacccttgca atagtgagac gtcttttctc ttccggactg
     3661 gctcggttgg gccggaagcc aagttcggct gcttcgtaag tgcaatgaca aaaaaatgac
     3721 aatcaaaacg cgtgccaaaa cacgttctga ttgtcatttt taatacacta tcaggcaaca
     3781 acttacgcga tcttatcctt ccccaagtcg ggccgcgggt aaattccacc tttcccgctg
     3841 caatcggcca aggtgaattc tactctcagc ccggggttga gggtcccgcc ggacatcact
     3901 cacactccag cggcccgccc gcacggaaga ggaaatcgat cagccgcgtc agatccgcca
     3961 ccgtcagatt cccatcgccg ttgacatccg ccgcctccgc caccgggggc atcggcccgc
     4021 cccggaacag gtggttgacg aggaatgtca gatcggccac attgggcgcc tcgccggagt
     4081 tgtcgacatc cccgcacacg taggggagct cgatcacgaa agagaacgac tcctcgtcga
     4141 agcccccgac cgcatcctcc acgcgggccg tgaagccgat cgggccggcc gcagtcggcg
     4201 tcccgctcag caggccgttg gccgcaaggg tcagcccggt gcccgccagg ccgccgcccg
     4261 gctcgctcca cgtgcggccg cccgttccgc ccaccgccgt cagcgtctgg gcgtacgcct
     4321 cccccaccac tccgttcggc aacgaccgcg tggcgatcga caccggcgca ttcatcagga
     4381 aggaaaacgc cttctcaacc gtgctgtccg cctcgtccgt gcacgtcgcc gtgaaagcga
     4441 tcgttcccga cgccgtgggc gtgccgctta ccagcccgcc ggaggacagg ctcagtccgg
     4501 tgccgagcag gtcgccgaat ttgtccgccc acgtccgcgc gccgaccccg ccccccgctt
     4561 cgagctgcac ggagtaggga acccccgccg tccagtccgg gagatcctcg gtcgtgatga
     4621 cgaagtaccc ttcctcgcac gtgtacgccg tcacgctcac ggcgtccacg gccgcctcga
     4681 ccaccgaccc gctgccgaga tcccccacgg tgaagcgcag ccgcatctgg tttgtgggcg
     4741 ccgtgaagtc cccgatctca aacgaatgct cgaaccaccc gcccgagact tgcgtcccgg
     4801 agggtccgac ccgttccacg agcgtccacg tgctgccgtt gttgttcgac acgtggacgt
     4861 ccatgtagtc ttcatagggg ctggccccga agtcattgga gtaccagcgc gcgtagtgca
     4921 cgagggcatc atcccccccg gtggcgtcga acgtcggcga ggtcaggtag gtcgtgcccc
     4981 cgtcgacgtc ggaatccccc gcgctgttgc cggtgaggta gcactggccc gagccgtcga
     5041 aatcggtcgg gggatcaccg cgcgatccgc cgcccgccgg cacgccccgc gcccactgac
     5101 cggcggtcgc gttccccgtc accgtccacc cctgcgccgt ttcaaaattg tcggccagcg
     5161 cggtggtgaa ttgcgtcgcc gccgccgccg accacggcga ggccgagtcc ggataggtgt
     5221 acatcccggt cgttacctcg gccgcgctca catagaactg caccctttcc ccgcagagcg
     5281 gtccaggcag cgggaccgcg tacgtgccgc cgccgagatc ggccagcggt ccggtctgcc
     5341 acgccccggc gtcgtaccgg aagtgcacct gcgcgctgcc gggggcaatc gccccgcccg
     5401 gaccgggcgt gatggagatc agaaactcgg acgtgtccac cggcggcacc atgaatggga
     5461 tcgggtcgac ttccgtgaag agcacccccg gcgtcggcag gtacgcgccg tccgcgtcgt
     5521 acgcggtcgc cagcgacgcc tgcacgatcc gccgcatgta cggaaaactc atataggtcg
     5581 tgctgtcctg ctccgagtgg tacacggtcg agaagttgta ctcgatgagg aagacgacgt
     5641 catacccttc ctgctggaac gggtagtggt cggagttcga ggccgatccg ctgaagtacc
     5701 cgatcaggtt cgtcgacagc gagtcagcga tgatcttcca gcgcgaggcc cacaccgtgt
     5761 cctcgccgta gtgcacgctc acgttgttca cgtttccgtt gtatccgatc atatccatgt
     5821 tcagcatcat gataatgctg tcgccgttgg ccgccgccga ggccgcgtag tggtaggatc
     5881 cgttcagccc ctgctcctcg gagtcgaaca gcgcgaacac ccacgtcatc tccgtcggta
     5941 tgtccgccat cacccgcgcc atctcgagca cggccgccgt cccggagccg ttgtcgtccg
     6001 ctcccggcaa gcccgacacc gcgtcccggt gcgccccgac cacaatgcgg tggtcgggga
     6061 gggtcgtccc catcttgacg ccgatgacgt tctggcagtt tgtcgagccg tagagaaagc
     6121 tgtcctggta cgcagagtca aggccgtagc tgatgaggcg gttctggagc cagtcgcgcg
     6181 actgccggtt ccccagggaa ccggtgaccc ggttggggaa ggcctggagc tggagtgtgt
     6241 acgactgcag cgagtcctgc cgcaccaggg cgatcagcgt ctcgaggtcc aggctccccg
     6301 gcgcgcgctg cttcacctcc cgaggcgccg gctcgcggta cacgattcgc cggctccctg
     6361 ggggcaacga cgcgagaccg tccgcctccg ccctgtcccg cacgtctccc ggctccaccc
     6421 ggtagaggcg cacgccgtcc tcggcataca ccgtcggata tccgcccggg ttggcgccgt
     6481 cgtggtgcgt gccgagcgcc agcgtgctgc ggtcgacagt ggtcgcgatc aggtcgtagc
     6541 gcagcccgct ctcggcgagc gccgggagag cccgggcatc gaccaggacg aggtatcccc
     6601 cccgcacccg tacggccgca tccacgccgg cggcgttcag cgcccgcgcg tcggccgcgc
     6661 tctccacggc cacacggtag aggtcggccg caccggccgc cggggcgatc ccgcacagga
     6721 tcgcacagat cacaagacat atccggtaac gcatatgaga catctcccat tcggccgccg
     6781 gtcgcggcct tgaagaacac ccccggggca ggcggccccg ggggtgtcac gagattatgg
     6841 tgcgcaggta ggcaggggac cgctgcggaa caggtacgaa atcaacatgg tgagatcgtt
     6901 cacctcgatc gccccggtgg cgttcaaatc ggccgccgcc atgatcggcg gcgcctcgcc
     6961 gccccggaac atgaacgcga ccaggtaggt gacgtcggcc accccgatcg ttccgtcgcc
     7021 gtcgatgtcg ccgcagatga acgcgggagc cacgtggagc gacagttcga cctggtcggt
     7081 cgacccgacc tgatcggccg cctggagcgt gagagtgaac gtcccgctgt cgaccggcgc
     7141 gccggtaatg aggccggcgt ccgagatcgc cagtccgagt cccgccagcg tgccgccggc
     7201 gtccgaccac accagcgcgc cggtcccgcc ggttccctcc atagcgtgcg tgtaggcttc
     7261 cccggccttg ccgtcgggga gactcgcggt cgtgatactc acggccggat taaccgtgaa
     7321 accgaacacc cgttcccccg tcgccccgac cgcgtcggtc gcgagcgccg tgaaaccgat
     7381 cgacccggcc gcggccggcg tcccggtcag cgcgcccgcg gcgttcagag tcaggcccgt
     7441 gcccgccagg ctgttgagct tgtcgctgaa tgtccgggcg cccgtcccgc cgctcgccgt
     7501 gagctgctgc gcgtacgcca cgccggccgt ccagtcgggc agcgtggcgg cgttgaccac
     7561 caccgcgctg ttgatcgtga aattcaggag ctgctgatca gtctgcgcat tttcgtccgt
     7621 gacgagcgcc gtgaacgtga tcggtccggc cgccgagggc gtaccggtca ggagcccgtc
     7681 ggtggtgagg ctcagccccg tcccgaccag gtcgccgttc agatcgctcc acgtcagcgc
     7741 gcccgtcccg cccgtggccg ccaactgctg gctgcaggcc acgccgatag tccagtcggg
     7801 gacatcggtc gtctgaatcg ccggcgccgg gatgccgctg ttgcaagtga agcgcgtcac
     7861 cttgaacgcg tccacccccg cctcgaccac cgagccgctc ccgagatcgc cggccacgaa
     7921 ccgcactttc atctgcgcgc tcggcgtgag gaagttcccc accaggaacg agtgggtgta
     7981 ccagccgccg ccggcctcat cgaccgggcc gaccgtctcc accagcaccc agctgttgcc
     8041 gttatcgttg gaggcgaaca cctccatcac gtcctcattc ggcgccgagc cgtatgtgtt
     8101 ggaaaaccag cgcgcgtagc tcacctcggc gtcgccgccc gacaaatcga aggtgggcga
     8161 gatcagcgtc gtcgtgccgc cgtcgatatc cgagtcgccg tcggtgttcc ccgtgaggta
     8221 gcactggccc gagccgtcgt agtcggtcgg cggatcgccg cggtcgccgc cgcccaccgg
     8281 cgtgccgcgc tgccactgcc cggccgtcgc cgtgctcgtc accgtccagc caaggttcgt
     8341 ctggaaattg tcatccatca cctccgtaac ctccgtcatc ggaatcgcgg agagcggatt
     8401 ggccatgctc gcttcttcat aggtcgtgcc gtcgctcagc cgggcgctga agtagaactc
     8461 gaccgtgctg ccgcactcgg ccggcgggag catcgccgtg aacgtatccc cggccgccgg
     8521 cgccatcacc tggtgctccc acgcgccgcc gtcggtccgg tagtgcagcc gctggctccc
     8581 ggtcatcatc tcgccgccgt agacgcccgc gatcaccacc gggaaaccgg tcgagtcggt
     8641 cggggtgatt gtcgacggca caccgtccgg gaatgagaac gcgagtcccg gcaggggcac
     8701 ggcgacattc accgctgtcc acgcccgcgc ggcccgctgc gaccacaggc cgctgggatc
     8761 gagatcatcg gccgcatcca gggtcgcaat ggccgcctcg tggtaggtcg tctggctgtt
     8821 ccagtagtag gcgttggccc ggtaggccac cctgatcgcg ttgtgcacgc cgatgccggt
     8881 gaccgtcacg ccgttgtgcg tcccgccgtc cgacagcagg aagaaccact tgttgccgac
     8941 gccgctgttg gtgtgcacgc cgcagtagtc gttccaggag ctcggcgtgc agttgactac
     9001 gtcaacccaa tacgggtcgc cggtgccgta ggtgtccgga tccccgaact cgtgcgggtt
     9061 gtccatcgac cggaacgcca cgcccgtggt ccggccgttc tcgcccatat cccagtcggg
     9121 gacgtccagc gtgtcgtagt agaactcgaa cgccgccccg atcatgtccg aaaacgattc
     9181 gttgagcgcc cccggctcct tctggtacac gaggttcgat tcatactctg tcaccgcgtg
     9241 cccccactcg tgggcgatca cgtcgggaca gccggccagc gagcgccacc cggtcgacca
     9301 gctccagacc acgatccgcg agccgtccca gtacgcgttg tcgttgccgt cgccgctgta
     9361 gttgacgatc gtgagcatgc tcgccccggc gttgttgtag ccgttgcgcc cgaaatgggc
     9421 cagcaggtag tcgtacatca gcgccgtgta cacctgcccg tccaccgccg gccgctgcgt
     9481 cgccgccaca tcccagtagt tgtcggcgtc cgtggcgatc gaccccggaa gcgtggagcc
     9541 ggcgatgttc gtttgaatat agttccccgc cggcatctgc ccgtcgtgcc cgtgcgggtt
     9601 gttggccgcc tgccgtgtgt agtccttcat ctggtaggtc gagccggtgt agtcgacgtc
     9661 gatatggtcg cgggccgcgc ccatcacgcc gatcccggtt ccgatcgcgt cgctgttcat
     9721 gatccggtta gccttgtaga tcaccgcgcc ggaccgggcg tcgatgaagt actcccaccg
     9781 ccccatcggc gtgtcggagt agaggaacag ccgccatgcg aggtgcgtcg tcccctccca
     9841 ggggaacacc accagctgcg gctcggccgg cgtgccttgg ccgaagaacg atgcgaggtc
     9901 ggtttccgcc agccgcaccg cctcggccgc ggccagctgc ggcgtgacct ccagagagat
     9961 cccgggtaca aacgtcccgt tgaccccccg gagctgatcc tgcgccgtga aatgcacgac
    10021 caggtcgccg ccgatcaccg gcagcccccg gtaccgctgc gtcatgcgta ggtgccgcat
    10081 ccccaggtca tccgtcacca gccgctcgac cgccagctcc gcgcggggat cggtcatgcg
    10141 gtacgatgct ttgtattcct caaagaagct gtaggccgac gcggcttcgt tcccgcgcgc
    10201 caccttctgc gcaaaagtac cagaaacata aagcggaacc ccgtcctcag actccaggta
    10261 cctggtcaca ttggggtcga ggcgaagccg gttggatccg gccggtccct cctcgtcatt
    10321 cgctgcgaag acagccccgg ccaccaacag gcagagcgtt accgttagca cgagcgcttt
    10381 cattgatcct ccaattgcgg gcatatagac ggttcgtatg cttcatccgg aatggccaat
    10441 ttcgcacatg tcaatcaggc aaggccaaaa aacgcggaga ctcgtcggcg gactaatctc
    10501 tatgtggcac aaatcaagat aacatattac ggggggaggt caaccaaaga atcgcccgcc
    10561 cacagacttc cgcgcgcccg tcccgttcta ccggatcgcc cgccgcagcc gcgtcgccgc
    10621 caccaccgca aggtagcttc ccccggccgc catgatgatc atggctccgg ccgggagatt
    10681 cggcccgaaa ctcgccgcca gccccgcgac cgtgaacaaa attgacagcc cgatcgatgc
    10741 ggccatgatc cgccccagcg tccgggcgaa ataccccgcc accgccaccg gcagcgtgag
    10801 cagcgcgatc acgagcacga tccccacgac cgtcaccagg atcaccaccg tcagcgccgt
    10861 cagccccagc agcagcaggt agtaggcctc gacattcagg ccgcggacgc gggcgaattc
    10921 ttcgtcgaaa cagacagcct gcaactggtt gaaaaacagc agggtcacgg ccatcacgcc
    10981 aagatccagc cccccgatga gccacaaatc aaccgggccg accatcagaa tgttgccgaa
    11041 taggtaactc atcaggtcct cattgtagcc gggcgtcgcc gcaatgaaaa ggatccccac
    11101 cgccatcccg atcgcccaga tcgccccgat caccgtgtcc tcccgctcgc gcgctttcag
    11161 gctcaccact ccgatcgtca gggccgccgc caaggccgcc gccaccgccc cgtagagcgg
    11221 cttgaccggc cagccgtgga ccagctccag ccaccgcgcc acccccatgc ctcccagcac
    11281 gctgtgcgcg atcccgcccg cgatataggt gatccgtcgc gccaccacga acgaccccac
    11341 catcccgcac gccacgctcg ccaggatccc ggtgaggaga gcgttcagca cgaacgtata
    11401 ctgcgtcacc gcgtggaaaa aatccagcat caggcggcgc cctccccgga cccctgttcg
    11461 acgtcgtgcc gcaccacgcg gaactccgtg ccgtacagct cccgcatgaa cccgctgtcg
    11521 agcgtggcgg tcgggtgcgt gtgcacgcgc cgcttcacgc acacgacgcg cttgaccgac
    11581 ccggccacaa acgccgggtc gtgcgacacc atgatgatcg tgaggcggtt gttcagctcc
    11641 tgcaggagcc gggtgagctc gttttctccc cggatgtcga ggttcgccat cggctcgtcg
    11701 agcaggagca gttcgggttc ggccgcgagc gcgcgggcga tcaggagccg ccgctgctgc
    11761 ccccccgaga gctccgcgag gttgcggttg cgcatgtccg cgagcccgac ctggtcgagc
    11821 gcccgaagcg ccgccttgcg gtcggcccgg cggtaccgcc cgatccagcg ccgcggtccc
    11881 agccgcccca tgagggccac ctcgaaaacc gtcaccggaa atttcggatc caaccgcgcg
    11941 tgctggggca tgtacccgat ccggtgccgc gcctcctccg gcggcgcccc gaacacccgc
    12001 accgtcccgc gtcgcggctg cagcagcccc agaatcagct tcaccagcgt cgtctttccg
    12061 cccccgttcg gccccacgat ccagacatac tcccgctccc cgatcgcgaa actggcctct
    12121 tcgaccgccg gctcgctatc gtagccgaac gtcacctgct ccagcaccac cgccgcccgc
    12181 tccgccatgg tcacttcacc tccggggccg tcccggccgc aatccgccgg gccatgtcga
    12241 ccaggttggg gaggtagtcg cgcgcgagcg gatcgatcgt caccagctcc gcccccaccg
    12301 cgtgcgccac cgccgccgcc gtcttcctgg caaactgcgg ctggacgaac agcgcccgca
    12361 cgctgtcggc gcgcgccgcg tcgatcaggt ccgcgagatc ccgggccgcc ggctccttcc
    12421 cctcccgctc aatcgccgtc tgccgcaggc cgtagcggtc gaggaagtag ccgtaggagg
    12481 gatgaaacac gtacacggcc cggccggcgc agggcgcgag gatttcgccg atgagcgcgt
    12541 gcacggagtc gagatcgtgc tcgaacgcgg ccagccgcgc cccgtagtcc tcgcggcgcc
    12601 ccggatcgag gcggcccaga gcctcggcga ttgtccgcgc ctgcgtcttc gccgccaccg
    12661 ggtccagcca gatgtgcgga tcgggcgtct tgccatgcgc cgcttcctcc tcgtccccct
    12721 cctcgtggct gtgcccctcg atcgaccgca gcgccacccc ggcgcgcgtg tcgattattc
    12781 ggtccgcccc cacaatttcc gccagccgtc cgatccaccg gttctcgatc ggcatccccg
    12841 cccggaaata cacgtcggcc gcctccaggc gcgccatctg ccggggcgtc ggctcgtagg
    12901 tgtgcggcga ctgacccggc ggcacgagca catcgaccgc cacgcaatcg ccgccgaccc
    12961 gctcgacaaa gtacgcctgc ggcgccaccg tgacaaacgc ctggatcgta ccccccgggg
    13021 ctttggcccc gctcccgcac cccgcgggcg ccagcccgat cgccaccgcg gcgcccaccg
    13081 ccccgatccc cctgccggtc cacttgatca gtctattctc tatcatcgtc aacctgttcc
    13141 caagtatgcg gcctccggcc gccgccgtct atttccctcg gcgcgtcctc ccccgccgcc
    13201 cagccttcac cgccccggcc gccggtttcc ggcccgccgg cttacggcct ggcctctgac
    13261 ccgcctcgtg ttcgcacacc aggtagagct cgtggcagtc gtcgcattcg gtgaccgcgt
    13321 ggtcatgcgc gaaccgatcc cagtggcgct gctgcgatgc cgaaagaatc cccgtcccct
    13381 ggcacagcgg gcaggttgcc cgaagcgcgg acagtaccgc cgtacggata aaggctgatc
    13441 gattggggac tttccggagt tcctccagca gcgccccgtc gaccttgaac gtcacgattt
    13501 ccgatttctt tctcatcgcg ccgaccggac catcccggtc cggcgaaatc ctcctgccgg
    13561 tcggagccca tgttaataca aagtaatacc ccgcgtcaag cgcttttccg cccccgtcgg
    13621 atcaacggcc ggagccgatt cttttggttc cggcgcccgg cctcgccctc gacccgcctg
    13681 ggccggaggc gcgcctggcg cggcggatca tcccggatac gagacttttt cggtcgtctt
    13741 aggcttgccc tgcctgttcg ccgtcaaaaa tgcgcctctc atgtccacaa atagcttgcc
    13801 taacgggggg cgacaagata tactagggca acctatccgg acaacccaca tcatagtgag
    13861 acaccaccgg cgttcggccg ttctgtgcgc agaacgtgag cagggataac tgtacatctt
    13921 tcccaggaga aggcgtcatg aagagaccac ggttcgtgtc aaaaaggggg gtcggccggc
    13981 gcacggtgca gagggatcgt ttggtttttc gggcgatgat cgtcgccgcg tccttggtcg
    14041 cggtcttgct ggtggccgcc gacggctgga gtgcgtgcac gcagccgccg ccgaaaatgg
    14101 ggctgtggtt gccgtttgac gaagcctccg ggaccgcggc gcacaatgtg cgcggtctcg
    14161 acggcgccct cgtcggcggc ccgtcctgga tcacgggcaa ggtcgacaac gcattgttct
    14221 tcgacggcgg caaccagcac gtcgccgttc cccattactg gacgaatccg ttcagcgcgg
    14281 cccgcggccg cggcatcgac atccgtgaga atgacttctc gatcgacgcc tggattcagc
    14341 gcgaggggct ggccggcatt cgcaccatca tcgacaagcg ccaggacatc ggcgggtacc
    14401 tcagcgggta cagcttcttc ctgctggatg gtcggctcgc cctccagctc gcctgcggcg
    14461 ccgccggcta caacaactgg gctgtgccgg cggggaacgc gattcccgtc tccggcacgt
    14521 ggcaccatgt cgccgtgacg gtcgaccgcg acagcccgac cgggctcgtg ttctaccttg
    14581 atggcgcgcc ggtcacgacc ctcaacccct ccggctacca gggcacgctt cacaatacct
    14641 cggagctctg ggtcggcgac cggattgttt cgccgcaggg cggcgcctgc ttcgcggggg
    14701 gtatcgatga attggagatc tttccgcgcg ttttaagtgc agcggagatt cagaaaatct
    14761 atgcggccgg ggccgatggg aagtgcaagg tgaccctggg cgcagaagtg tatgacggcc
    14821 cgtcgccgac aggatgggcc aaattaaccg gtctgattat caattccggc atcgagccgc
    14881 tcacggacga ctattcattc gcaggagtgc cggacacatt tccaattgag ttcacgccgg
    14941 cttcgggcag cgtgacggtg ccggctcggg aagtcaccat gattggatct gaggccatag
    15001 tggatactca ggacattcag cattgctggc cgtaccagat gttctttaac ggcttcgccc
    15061 ccgacggcga caccctatgc atcgactcca tgtggtgcgc aggattcgaa aagggcttct
    15121 ttgccgcagt gaccgattgt gtcgataatc tgggttcgct gaagatattc aacgccggct
    15181 ttccagacac gttcccaata gattttagga ttgcagccgc aatcgattac tcgtaccaag
    15241 gtgacacact tatgatcgac agcatggggt acgccccctg cgtgagtctg aacgggctca
    15301 accccggaca gcctgtttac ggcaacgcca acgtcctgcc cggcgacagc gtgaccatta
    15361 ctctgaccgc caagttcgtc gactacgcct tgcttgaccc gtacatgatt ttgctccaag
    15421 tctaccggag cagccagtgg cagacgttgg cgtcaatgta tttcgtcaac gtgctcacgc
    15481 ccccgagctg ctgcgtcgcg cgcggcgatc tcacagggga cggcagcctg aaggtgtccg
    15541 acctgacttt cctcgtgaac ttcctcttcc ggggcggtcc atcgcccaac tgcccggatc
    15601 atggcgatgt caacaatgac ggcggcataa aggtctccga cctgacctat ctggtgaact
    15661 ttttgttccg cagcggaccc ccgccgccgc cgtgcgactg accgcggcat gccgtggagc
    15721 agagcacggg ggcaagcgac aacctctgcg attttcattc gcatcgtacg gcgggagccc
    15781 gcaggctccc gccgcctcat gcagctccag tccccgtgaa tcttgagccc gcaaggcggc
    15841 gcgcgacgag gccgtccgac caccgctcac gtgacccgtc ccccagtggt tggcccttct
    15901 ctaggtcagg atccttgtgg tcctgacatt cttactatcg atgtgagcgg tcccgtcagg
    15961 accataaagg tcctgaccta caaaactacg cgccggctcg gtgcccgcct gcgcagacgg
    16021 tcgcgcacag tctgtggttg acgcggggcg atccccgccg tatcttcgtc gatcagatct
    16081 tcaaccggat gcgcgagaaa tgatatcggt cgaacacctg cacaagcgat tcggagacat
    16141 cgcggccgtc agcgacgtct cattcgacat cgccgccggc gagaccttcg gcctgctcgg
    16201 gccgaacggt gccggcaaga ccaccacgat cagcatgatg gtcggtgtgc tggcgccgga
    16261 ttccgggcgg gtgcgtctgg acgggatcga tgatccgacg cggccggcgc tgcgccgaaa
    16321 gatcggcaac gccccgcagg cgctcgcgct ctacggcgac ctcaccgcgg ccgagaacct
    16381 ggcgtttttc ggccgcctct acggcctctc ccgggcccgg ctcgccgcgc gggtcgcgtg
    16441 ggcgctcgag ctcgccggtt tgacggaccg ccgcagccac ctggtgaaga cgttttccgg
    16501 cggcatgatg cgccgcctca atctcgcctg cgccgtcgtg cacgatccgc cgctcctgct
    16561 tttggacgag cccacggtcg gggtcgaccc gcaatcgcgc aaccagatct tcgacaccat
    16621 cgaggccctc gggcgcgagg gacgcaccat cctctatacg acacactaca tggaggaggc
    16681 ccagcggctg tgcgaccgcg tcgccatact cgatcacggg cgtattctcg cgctcgacac
    16741 ggtcgaccgg ctgatccaca agcacggcgg gaagtcggtc atcgaggccg aactcgaacg
    16801 cccgccggat ccggggcaac cgctgcccgg cgacctcacc ggcacctccc tgcggctgga
    16861 gaccgagcgg ccgctcgaag agatcgcggc gctggcggac gcgggcgtgc gctttctccg
    16921 cctcgagatt caccgcgccg acctcgaaac ggtgttcctc aatctcacgg gacggaggct
    16981 gcgcaactga tgaagacgct tttgactctg gcccgcaagg atctccgcct gctctggcgc
    17041 gaccgcttcg gcctcttctg ggtgatcgtg tttcccctgc tcatggcgct ctttttcggt
    17101 tccattttca gcaccggcgg ggcgcgcgcg ggcagcatga aaatcgccgc ggtcgacgaa
    17161 ctgcggaccg agcagagccg gcagatgatc ggcaagctct ccgccaagcc cgtgctcgcc
    17221 gtcaccccgc tgccgctcga ttcggcgcgc ctgctcgtgc agaaggggaa gctcacggcg
    17281 tacgtgctcc tgaagcggga ctcgcttgcg accggcgagg gcaacccctt cgccatgccg
    17341 ccgatcgaga tcggtctgga tccgtcgcgc aaggccgagg ccggctacct gcaggggatg
    17401 gtgaccgagg cctggttttc gctcatgcag gaccgcctgg ctgacccggc cgtcgcgaaa
    17461 acctgggtca acgagggcct ggcgggattg cgcgccgaca ccgccctgcc ccccgaccag
    17521 cgcggcgtgc tgacccggct gcttgcgagt ttcgacgact atctcacgac cttcgattcc
    17581 gccgaggtga gccaatcctc cccgttcggg aaaaccgcca tcacggtggt ggaggtggcg
    17641 gtggaacggg aggggccgcg gtcaccgttt gagatcacgt tcccccaggc gctcacctgg
    17701 gcgctgctcg ggtgcgcgct gacattcgcg cagtccatcg tcagcgagcg gcagcgcggc
    17761 acctatctgc ggttgcggct cgccccgatc acgcgcggac aaattctcgc ggggaaaggg
    17821 ctggcgtgtt tcgtcacctc cggcgcggtc tcggtggcgc tgctggcggt gggagtgctc
    17881 atcttcggcg tccgcgtggc gttcccgctc ggcatggcga tcgccctggc ggccgcggga
    17941 tgctgctttg tcgggctcat gatgctcatc tccgtcctcg gccgcacgga gcaggcggtc
    18001 agcggggccg gctgggcgat catgcttgtt atggccatgg cgggcggctc gatggtcccc
    18061 ctcatggtca tgccgtcgtg gatgctcacc gtgagcaact tcagcgtcgt caaatgggcg
    18121 atctacgcgt acgagggagc gatctggcgc gggttcacgc tcggacagat gctcgtgccg
    18181 gcgggcattc tcaccgcgat cggcgcgatc gcgttcgccc tcggcgtcgc cattctggcg
    18241 cgcactgacc gctgattccg cctaaccgca aaaaaaggac tcctgcagca gggccgcagg
    18301 agtcacagct tgtgcgcggg tcgatccgct acttgacgat cttgaagcag tagggcccgc
    18361 cgaagtcggg ctcgttctcg acctgatcct ccaaagacca gagccagtag ccgccgggcg
    18421 ggaaaaacga ctggtcgagg cagaccgtct tgccgaccat cgacgccgga atggggccga
    18481 tcgtgatcgt cgccggcacg tcgctccagc cgtccggcaa gccgtctttc agaatccgga
    18541 agccgccgaa accgacggtg tcggcgcccg aaccgctgat gccgaaagtt gtgatgaaca
    18601 tgccgccgtc catcaacccc ttgggcatca gtccggtcgt gtcggccacg gtcgtccccc
    18661 acgtcgcccc gtcgggcgag tagacgcgga aaccgttggc gaatccggcg accggcaggc
    18721 cggtcttgtt cgtgaagcgc aggtggaaag tgatcttctt gcctgcctgg atctgcccat
    18781 ccgcaaccaa gccgtcgatc ttgtcgatgc tgatgctgcc gccgcccacg gcgcggtggt
    18841 cgaacgggtt cttcgacacc ttgccggcgg ccgtcttttc ggccatgagg gcgggcggtg
    18901 cgaggagcat gccgcagagg gtcagaacaa cggtgagttt tctgtgcatg taaactctct
    18961 cacttgatga gtaacgttcg cacgtatcgt gctgccatga tccttaactt acaacgccgg
    19021 cggcccgatg tcaatggccc ggccgtttcc ttatacaggc cagggaccgc gaatcgccgg
    19081 aaactttttg cggcggctct ccggctgcat gccgtgtcgg cagcggcccg ccccggcggc
    19141 cggcggcgcc gcgggcccct gcaaagtccc cattgtggct caggtcgatc cgatcttcgt
    19201 catcttaatc gtcccatatg ctgtcggcat tgtcccggtt ggactgcacc cgtcgcgccg
    19261 ccgcaaatcg ccgaccggcg atttcgcccc ggccaaccgg gaccgggggc cgccgacgtc
    19321 ggctcctgcc ctcgcctcgc cggcagcgcg gattgcaggc ctgccggcgg cttcgggaaa
    19381 gaaagcctgt tccgacccgg cgcgtgcgcc gcggccgtcc gccgccgaac aaaacgatgc
    19441 gcggcgggca gaccgcccgc cgcgcgcgaa tctcttcacc ggcgccgccg caggctcacg
    19501 gcgacgagag gatgagggcg acgttttcgg ccgtgccgat atcgatctct cccaccgtca
    19561 caaaccggcc ggcgccccag gccacaccgt gcaattcacc ggcgaaacct gcggccccca
    19621 cactccaggt cacggcatcg gcggagtaga gcgtcacgat gacatcctcg gcggccgctc
    19681 cggcattgcg cgagcccgcg gcgacccagc gcgcgccgct gaaggcgagg ttgttgatcg
    19741 cgtacacggc cgtgtccccc ggccgcgcct cgaatacgtt ccacgccgac ccggtcgtcg
    19801 aggtcaggat gcggccgttg ctgctggccg cgacaaactg gccgttgccg aaggcgacgg
    19861 cggtcaggtt gagcggcgtg gcggcgtcga ccgtatcggc cgtccacacg ttgccgtccg
    19921 tcgaagtcag cacgaccgac gccgtgccga ccgccacaaa caggccgttc gcccaggtaa
    19981 cgccgtggag cgggttcgtc accgggacat cggcggcgac ctgccaaagg gccgcaggat
    20041 cctccgacca gattatccgc ccctggtcgc ccacggccac gaaccggctg cccgaagccg
    20101 ccaccgagtt gagctgcagc tgctcgccgg ccggcggaac cggccgctcc cagtgcacgc
    20161 cgtccgtggt gaccgcaatc acgccggcc
//
LOCUS       DAOPOE010000058        20022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3323_length_20022_cov_4.785696, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000058 DAOPOE010000000
VERSION     DAOPOE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_20022_cov_4.785696"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PKM94_08785"
     CDS             complement(<1..834)
                     /locus_tag="PKM94_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD66772.1"
                     /translation="MANAINLLVLTHNYPRRAGDHAGVFIALLAKHLEPHGLRPIVLA
                     PHDPGAAEREDLEGVRVHRFRYAARDQDEDVAYRGHMQHIVLGSVSGVFRFRRFLHCW
                     RAAAFDLLVRERIDAVAGHWLVPSGFVLKTIADRTTLPLVLSSHGTDVRLIRKFAGAA
                     YRYFRPLGRRLDRWTFVSTFLRDAIAGLDPGLAPILEVLPLPHDETVFHRDPAVPREP
                     HTIVAVTRFTPQKRVDALVKAFALVAAHRPDTRLEIYGAGPGEHGIRALIDRFGLGER
                     VR"
     gene            928..1857
                     /locus_tag="PKM94_08790"
     CDS             928..1857
                     /locus_tag="PKM94_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOD66773.1"
                     /translation="MKTRTPIDFALDLDFREILTNPILDIAARVWEDDRYEAFRVCYR
                     SMRIIDDLVDNQKAGGRTLAVEEQCLFASMIDDWINSLRRGRPADAFQEQFLETLHRY
                     RLPLWPWEKLAEAMVYDLYHNGFATFREFLRYTEGAAVAPASVFMHLCAVRPHATGIL
                     EPAFDIRRAARPLAVFSYLVHIMRDFRKDQTAHLQYFADDLMAEEGAAPEDLQQAARG
                     GEIVRPVRDLMGRYRELAERYRRISRRMLDRVQPDLAPRYGLSLEIIYQLYLQIFERV
                     DPRYGDWSAAELQPPPAAVQARIEATIRTFRAA"
     gene            1980..3323
                     /locus_tag="PKM94_08795"
     CDS             1980..3323
                     /locus_tag="PKM94_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOD66774.1"
                     /translation="MARLVGIICVGVLTAAAAGAEEVAVTVYNSNLGVVSEVRDLSFV
                     TGTGEVAFRDVPDQIDPASVRFELVGSRQGISILEQNYAYDLVSVDKIYSRYVDYDIE
                     VLDKQGNLIGGTLLAAGQGAITLRDASGRVKVVLLENVTQVNFPTLPEGLITRPTLFW
                     RYAAETGGSFPARVSYQTYGLSWEAEYVGVLSADEKTLDLSGWASITNNTSKTFADAT
                     LTLVAGDISRAPQAKAAGMARMDMLATAEAGFEEKAFFEYHLYTLPRPSTVAGKEVKQ
                     ISLFEPARTAAEKRFVYRPEQNAQQVEVALQVVNAEQAGLGMPLPEGRVRVFQADADG
                     SLILLGEDRINHTPRDEKIDIRVGYAFDIVPEEKLMNQTRVSQQVEERAYELQLRNRK
                     DEPVTVHVEKALPGDWQVIAADFRYERKDARTLTFDVPVPARDTTTLNYTVRVKW"
     gene            3428..4816
                     /locus_tag="PKM94_08800"
     CDS             3428..4816
                     /locus_tag="PKM94_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66775.1"
                     /translation="MIVIVMLAAAALLAGSSLGGGKPLKAMISGAKIAIIEGRPDEAL
                     LLLDTVAISFGAVPEAYQWTFRVYADKMEGAAGLDAKRPHLVKMLAYIDSLHLTCEGK
                     IPVQDKKYLKDCAKYIQLADSTKVKYFRFFYNLGHGQVKTLEQLAGSLEGETDSTRLA
                     YIRDQINTNIDSVIANMSMAILIDSSDYSPYVAIGDSYDRAGNYEKAIEWMTRGYERA
                     KNPLLLSQQLAYYHVRQDDYCGAIPYFQEYLKAYPDSLNTMNYLAACYNNCGLAPEKR
                     IYVDSAMTIYRVILERAPENPNVWAAGGRYFLIKAQDMSDSASAARTRQDAAAAAQFD
                     LLRRAMFDSSRTYFRTAWEQAPADVAIAEQFAFTSALLEDCPAAVKGFEVVAEAKPDN
                     AENWISLGDCYLRMADFPNAVRAYEKVAALQPGKLKIWENLGALYKETGQADKAAEAA
                     KKVQELSAAGQG"
     gene            4899..7304
                     /gene="priA"
                     /locus_tag="PKM94_08805"
     CDS             4899..7304
                     /gene="priA"
                     /locus_tag="PKM94_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOD66776.1"
                     /translation="MAARHASGGPYVNVAVAGPMQRTFTYALPPGSAPLSPGQRVLVP
                     FGRSRTVGFYLGPAGRPPEGIAAKPLGRAIDPYSYFSDELFALCVWMAEYYLANPADC
                     LSAALPPTIKNRAEAHLVWTEAAAEAAPELRDLIRPGRQLRPSDLTALRRRRLPVARL
                     VAAGAVVERWPEANAHTGQPVVGYRAARLPAWEEWFARAKVKPYPFDGMKSRAELLAA
                     GWTAHYVAKAIREGLLEPCYREETADLYDMIAPRENLEQIRLTHQQEAIFRQLRPAAA
                     NPAFGVHLLHGVTGSGKTIVYCHLAREVVAAGRTVLVLTPEIALTGTTLAYFRGFFGD
                     RVTVIHSAMSDRERLASFNGIRAGTYPIVIGPRSAIFAPLAHLGLVIVDEEHDPSYKQ
                     DDPAPRFHGRDCAIMRGRIKDVPVLLGSASPSIESYYQAQTGKYRLLELTERPAGATL
                     PAVRVIDMKTDRLGGDLPFLSYSLKKEVDERLARDEQVILFLNRRGYSPQLKCSRCGR
                     VPGCPNCQVKLTYHKEGRKLSCHYCGWVEQRYDACPSCGDPHFFPVGAGTQRVEETIP
                     RLFERAAALRLDSDSATGRRRLHEILTDFSAGKANLLLGTQMVTKGLDLPGVTLVGVL
                     AADALLDLPDFRASERAFAQLLQVAGRSGRAAKPGEVLIQTYDPEHPVIDRAAAQDYR
                     GFYESEIRKREELGYPPYSRLVNFVLSGDDAKRLEEAALAFREELDARMREAGVNGRV
                     LGPAPCPLYYLRSTYRRHLFVKTRQIRALVVMLSAWEQQTARFGLPAKIKVVVDVDPN
                     DMM"
     gene            complement(7822..8544)
                     /locus_tag="PKM94_08810"
     CDS             complement(7822..8544)
                     /locus_tag="PKM94_08810"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOD66777.1"
                     /translation="MKEVPIAARPRDRFGKGPARRTRMAGEIPAVVYGPEVESRTIAL
                     GEREFHATLRAGSGTTIFNLAVEGVTNRVILREVQRDPVSSRITHVDFYAISESKPLN
                     IDIPLTFVGTPKGVKDEGGIMQITMRDIEVSCLPKNIPEVVNVDVSNLGIGDAIHVSD
                     LTIPNVEILAESDRTVVVISAPTVIKAVAAEAAEAAVAAEGEAAPAAEGEEGAEGAKD
                     KDKEKEKGKEKEKGKEKDKGKD"
     gene            complement(8593..9552)
                     /locus_tag="PKM94_08815"
     CDS             complement(8593..9552)
                     /locus_tag="PKM94_08815"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOD66778.1"
                     /translation="MIVREEIRLVTGNANRPLAERIAAYLGEKLTDCTVTRFSDGEVF
                     VQINDNIRGADVFVIQSTNPPAENIMELLMLIEAARRASAMRITAVIPYYGYARADRK
                     DRPRVAITAKLIANLITTVGADRVITMDLHASQIQGFFDLPHDHMYSSGIFNKYFRTL
                     NLDPLVVVSPDVGSIKLARATAKALNAELAIVDKRRPRPNQAQIMNLIGNVEGKNILI
                     RDDMVDTAGTICQAAHFLKERGARDIYAAATHGVLSGNAITRIIASPIKTMIITDSID
                     HSGKPLTDKFEILTCSELIGEATKRIFDEESVSSLFEDQPLVV"
     gene            complement(9666..9737)
                     /locus_tag="PKM94_08820"
     tRNA            complement(9666..9737)
                     /locus_tag="PKM94_08820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9703..9705),aa:Gln,seq:ttg)
     gene            complement(9790..10059)
                     /locus_tag="PKM94_08825"
     CDS             complement(9790..10059)
                     /locus_tag="PKM94_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOD66779.1"
                     /translation="MEITEVRVTLREEERLRGFANVTFDNAFVVRGMKIIQGNTGFFV
                     AMPSRRRPNGTHQDIAHPVNAEMRLAIEERVLAAYEEELKAHARV"
     gene            complement(10075..10938)
                     /locus_tag="PKM94_08830"
     CDS             complement(10075..10938)
                     /locus_tag="PKM94_08830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOD66780.1"
                     /translation="MRVEAPAKINLFLQVLNRRPDGYHNINSLFQAVSLRDTLTVALA
                     DAPGVRLDVIGEDGLPAGEDNLVVRAARLMAREFGLNQGLHIVLEKRIPVAAGLGGGS
                     SDAAATIEAINHLFGLRLAPPDRMRLGLLLGSDVPFFFTRGQALVRGRGELMEETEFP
                     TDYGLLLVNPGFPLSTATAYAALKRNLTDSGVPFNLPCCRAVDEYVHHLGQADNDFES
                     IHRRSHPELDEIAGELLKRGAVLARMSGSGPTVFGLFASEPEHQDLVVQRRGNWRCYA
                     VAPITLLSQES"
     gene            complement(11336..12019)
                     /locus_tag="PKM94_08835"
     CDS             complement(11336..12019)
                     /locus_tag="PKM94_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66781.1"
                     /translation="MIHVHCAIYSLLCLLVLDAKGVGRDSFPVHVPPAAEEPRTALLG
                     LDGVTVAVCMSPGALYAFGTEELFRAQMRLTLAHHRLAVYDAPGRLDRDSMHMNIPDL
                     VYSIDFIRNRLDDGSPTGLVTYVLTLSARDYLDYARFEKSRTGVKLRGLPPVYCSFWE
                     RSTFGLAGEDDFKDIAKERLSEFLDVFIAEWQQCNSPAALRWLEAGNAQRNSPSLEKG
                     QGEEQTTGR"
     gene            12294..14990
                     /gene="acnA"
                     /locus_tag="PKM94_08840"
     CDS             12294..14990
                     /gene="acnA"
                     /locus_tag="PKM94_08840"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOD66782.1"
                     /translation="MDPFGVKGKLKAGGNSYTVYRLDKLAGQRGIDRAAIERMPYSIK
                     VLLEAVLRNADGRVIRDEDVAAMARYDARKAAEQSHEFPFKPARVLLQDFTGVPAVVD
                     LAALRSAMKRMGGDPKKINPLVPVDLVIDHSVMVDRYGSDDALAANMEMEFQRNAERY
                     EFLHWGQKAFANFRVVPPATGICHQVNLEYLAKCVLVRDGEAFPDSLVGTDSHTVMIN
                     GLGVVGWGVGGIEAEAVMLGQPLYMLTPEVIGFKLTGKLAEGTTGTDLVLTVTQMLRQ
                     KGVVGKFVEFYGEALDTLPLPAKAMVANMAPEYGATMGFFPVDELTLDYLRMTGRTDG
                     EVDLVECYCKEQGLFRAKGAAAPAFTDTLELDISQVEPSLAGPKRPQDRVALSKMKDE
                     FQRALARPIKERGFELKPSETERQVKISDGYRAAIGHGAVVIAAITSCTNTSDPSVLL
                     GAGLLAKKAAARGLTSKPWVKTSLAPGSRVVMDYLEKAGLIEPLKKLGFHNVGFGCTT
                     CIGNSGPLPEAVVKAIEEGNLVVSAVLSGNRNFEGRVNPHTRANYLASPPLVVAYAIA
                     GTTAIDLATEPIGTDEGGKPVYLRDIWPSQREIAEAMAGAISPAMFRARYGAVFTGNE
                     RWNRIQSPASELYRWDEKSTYIQEPPFFVDMKPEPGDVEPIRDARVLGIFGDSITTDH
                     ISPAGSIKADSPAGQYLLSHGVTFADFNSYGSRRGNDRVMTRGTFANIRIRNLLAPGT
                     EGGVTIYLPANQQMSIYDAAMKYQADGTPLLVIAGKDYGMGSSRDWAAKGTSLLGIRA
                     VLAESFERIHRSNLVGMGVLPLQFLPGEARERLGLTGHELYTIEGLSNDMKPKQRVTV
                     KALSKNGEKRFEAVARLDTPIDVEYYRHGGILQMVLRQLARA"
     gene            complement(15024..15770)
                     /locus_tag="PKM94_08845"
     CDS             complement(15024..15770)
                     /locus_tag="PKM94_08845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOD66783.1"
                     /translation="MNLIILTEHDRVGGETFRLAAQRADHLRTVLRATEGDLVQVGLL
                     NGPCGTARVARLAADEALLDCRWTAPPPAPVPTVDLICALPRPQTLKKILFAAAMMGV
                     RRLHLVRARRVERSYFSSPLLNPEQYTPHLIEGLSQGRLTRLPEVAVHDRFRRFFEDI
                     LPVLEQNESAPSLRLAPHPGAAAMLDAVYRGDSPRLLAAVGPEGGWVPFEVELLTSLG
                     FRAFSLGRFTLRVEHAVTAVLSQIELLAPR"
     gene            15996..17093
                     /gene="gcvT"
                     /locus_tag="PKM94_08850"
     CDS             15996..17093
                     /gene="gcvT"
                     /locus_tag="PKM94_08850"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOD66784.1"
                     /translation="MSEAVKTAFYDYHIQAGAKMVEFAGFCMPIQYSGITDEHLAVRT
                     NVGLFDLSHMGEFEVSGPDALAFLQRTTTNNVADLQVGQIQYSCMTMEHGGIVDDLLV
                     YRMGPQEYLLVVNASNIAKDFAWLQSHLAGNVRLVDQSPATSLLAVQGPKAEEMMRQV
                     TSFDLGAMPYYTWARAEIGGVKVLFSRTGYTGEDGFELYIPPPDTDRLWRVLTEAGKK
                     FGMKLIGLGARDSLRLEMKMALYGNDIDDTTTPVEAGLSWIVHFDKEFIGKPVLEKQR
                     DEKPVRRLVCLELEGKAFPRHGYTITDGAKPIGQVTSGTFSPSLQKAIAMGYVPRDLS
                     KVGSTVRVAIRGKEFPAVVVKPPFYKHGSHK"
     gene            17101..17859
                     /locus_tag="PKM94_08855"
     CDS             17101..17859
                     /locus_tag="PKM94_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOD66785.1"
                     /translation="MQVDLYLTPIPFGRAKLSGRTVVAIDVLRSSTSTCAALLAGAKG
                     VIPTPGPGEAGELWAKLGGDMAVLAGERDNVRIENFKLGNSPAEFTPETVGGKFVIMT
                     TTNGTGVYRDAVGADLVLCCALVNVSAVARKAAAAGHGLVLVCSGQEGGFSIEDTICA
                     GMLVHLLATEHKLNLALNDAGSLALLLYRSNKSALKKTIQQGEHGRRLTELGFGADVE
                     TAAAVDTMPVLPVMVDGRLVADVDPPAAPPAEPA"
     gene            17896..19797
                     /locus_tag="PKM94_08860"
     CDS             17896..19797
                     /locus_tag="PKM94_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66786.1"
                     /translation="MRHLIILAALVSLEAGAPAAAADAVRFALDVHQQDSAAGAVLLY
                     SDTATVVRGVAAAGFALAFSAEVEVTAADTAHAQFLVHAVTLGPSARTYARSYSAEYG
                     LPARIEGIEGKGGGRLMLTVTPLAFTDFDTAQCLFDHRVTGAYKIQPSASLDVHYVPN
                     SFGDYYIEAFKGVLEHHYRQFQEVFQFSLPGKFSIFLLPCASPAVLWDTRYGQVTDPT
                     RSTAFAIAANGQNTVDPFIATHTALLRSWGYAPPFLSEGAANYTSFALFDMKKLAAEG
                     RVPPLDSLLDTYAYLTAAPLVADRASATFAQYLIDSYGLEAFRALYVSADDLNLRRRL
                     ALAYGKGIGALEAEWRAYVDTSTIRFPALAQEADKAEALFQYGRMRDYARAMLELAPG
                     RIDSAFAWSRLKQAEFMLGNYYGAADAAEAQVQLDGERAVNWMTLGSFQMMNGMYAEA
                     RASLQNGQSKDPSDQTIAFNRALAALYTSDTATAESLLVEIITRPESRAPQGEARVVL
                     GEILLSRGDKRGRDRAATYFVEAMQTFQQQLSVNRTMPTAYLWLGAAALGMGDTELAR
                     DALETGLFLESRPFYLGQMNLWLGKTADVLGDHGLAQEYYGQVLAGSSAAYAQQEARR
                     CLEKPYRIK"
     gene            19804..>20022
                     /locus_tag="PKM94_08865"
     CDS             19804..>20022
                     /locus_tag="PKM94_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOD66787.1"
                     /translation="MFNFLNSGVLFAALAALIPLLIHLFSKRRVKIVEFSSLKHLKEM
                     QRRQVRRLKIRQILLLLLRMAIILALVLA"
BASE COUNT         3545 a         6064 c         7033 g         3380 t
ORIGIN      
        1 gcgtacgcgc tcgcccaggc cgaaacggtc gatcagggcg cggatcccgt gttcacccgg
       61 accggcgccg tatatctcca ggcgggtgtc ggggcggtgc gcggcgacga gagcgaaagc
      121 tttcacgagg gcgtcgacgc gtttctgggg ggtgaagcgg gtgacggcga cgatcgtgtg
      181 cggttcgcgc gggacggccg ggtcgcggtg gaatacggtc tcgtcatgcg gcagcgggag
      241 gacctcgaga atcggcgcca gcccgggatc gagcccggcg atagcgtcgc ggaggaaagt
      301 cgagacgaaa gtccagcggt cgagccgccg gccgagcggc cggaaatagc ggtaggcggc
      361 gccggcgaac ttgcggatca gtcgcacatc ggtgccgtgc gacgagagca cgagcggcag
      421 ggtggtgcgg tcggcgattg ttttcaggac aaacccggag gggacgagcc agtggccggc
      481 aaccgcgtcg atgcgctcgc gcacgagcag atcgaacgcg gcggcgcgcc agcagtggag
      541 gaagcgccgg aaccggaaga cgcccgatac cgagccgagg acgatgtgct gcatgtggcc
      601 gcggtaggcg acgtcctcgt cctgatcccg ggcagcgtag cggaagcggt gcacgcgcac
      661 gccctcgagg tcctcgcgct cggcggcgcc gggatcgtgg ggggcaagca cgatgggacg
      721 cagaccgtgg ggctccaggt gtttcgccag gagggcgatg aacacgccgg cgtgatcccc
      781 ggcccggcgg gggtagttgt gggtcaggac gagcaggttt atcgcgttcg ccatcaccgc
      841 agagtaggaa aagggccgcg gccgcgccaa atataatttt cgggcggcgc gcttatgtgt
      901 tatactccgg cttactcggc ggtgactatg aagacccgga ctccgataga tttcgcgctc
      961 gatctcgatt ttcgcgagat tctgaccaac ccgattctgg atatcgcggc ccgggtgtgg
     1021 gaggacgacc ggtacgaggc cttccgcgta tgctaccggt cgatgcggat catcgacgac
     1081 ctggtggaca atcagaaggc gggggggcgg acgctggcgg tggaggagca gtgtttgttc
     1141 gcctcgatga tcgacgactg gatcaattcg ctgcgccgcg gccggccggc cgacgcgttc
     1201 caggagcagt ttctggagac gctgcaccgc taccggctgc ccctgtggcc ctgggagaag
     1261 ctggccgagg cgatggtgta cgatctgtac cacaacggat tcgcaacctt ccgggagttt
     1321 cttcgctaca cggaaggggc ggcggtagcg ccggcctcgg tgtttatgca cctgtgcgcg
     1381 gtgcggccgc acgcgaccgg gattctcgag ccggcttttg acattcgccg ggcggcgcgg
     1441 ccgctggcgg tgttctccta cctcgtgcat atcatgcgcg atttccggaa agatcagacg
     1501 gcgcacctgc agtatttcgc cgacgacctc atggcggagg agggagccgc ccctgaggat
     1561 ctccagcagg cggcgcgcgg cggagagatt gtgcgcccgg tccgggacct gatggggcgg
     1621 taccgggagc tggccgagcg ttaccggcgg atctcgcggc ggatgctcga ccgggtccag
     1681 ccggacctgg cgccccgcta cgggctcagt ctggagatta tctaccagct ctatcttcag
     1741 atattcgaac gagttgaccc gcgctacggg gactggtcgg ccgccgagct gcaacccccg
     1801 ccggcggccg tgcaggcgcg catcgaggcg accatccgga ctttccgggc cgcctgagtt
     1861 tgcactttct ggggaagaaa ccccgcccta cattggtagt gatgctgacg gcggcggaag
     1921 gatccggcga aaggccggcg tgaacgccgc cgggaaggtg tataacagtg gaggaacgaa
     1981 tggctcgtct agtcggcata atttgtgtgg gcgtactgac ggcggccgca gccggcgcgg
     2041 aagaggtcgc ggtcacggtg tacaacagca acctcggcgt ggtgagcgaa gtgcgcgacc
     2101 tgtcgttcgt cacggggacg ggggaagtgg cgtttcgcga cgtcccggat cagatcgatc
     2161 cagcttcggt gcgtttcgaa ctggtggggt cgcggcaggg catttccatt ctggaacaaa
     2221 actacgccta cgacctggtg agtgtcgaca agatctactc gcgctacgtg gattacgaca
     2281 tcgaagtgct cgacaagcag gggaacctga tcggcgggac gctcctggcg gcgggccagg
     2341 gagcgatcac gctgcgggac gcctccgggc gggtgaaagt ggttctcctg gaaaatgtca
     2401 cgcaggtgaa tttcccgaca ctcccggagg ggctgatcac gcggccgacg ctgttctggc
     2461 ggtatgcggc ggaaacgggg ggatcgtttc cggcgcgcgt gagttaccag acctacgggc
     2521 tgtcgtggga ggcggaatat gtcggggtgc tgagcgccga cgagaagacg ctggacctct
     2581 cgggctgggc ctcgatcacc aacaacacga gcaagacgtt cgcggacgcg acgctgacgc
     2641 tggtcgcggg ggatatcagc cgggcgccgc aggcgaaagc ggccggcatg gcgcggatgg
     2701 acatgctggc gaccgcggaa gcggggttcg aagaaaaggc cttctttgaa tatcacctct
     2761 acacgctgcc gcgcccgtcg acggtggccg gcaaggaggt caagcagatt tctctgtttg
     2821 agccggcgcg gacggcggcg gagaagcggt tcgtgtaccg tcccgaacag aatgcgcagc
     2881 aggtcgaggt ggcgctccag gtggtcaatg ccgagcaggc ggggctgggg atgccgctgc
     2941 cggagggacg ggtgcgggtt ttccaggcgg atgccgacgg ttccctgatt ttgctcgggg
     3001 aggaccggat caaccacacg ccgcgcgacg agaagatcga catccgcgtc gggtacgcct
     3061 tcgacatcgt gcccgaggag aagctcatga accagacgcg cgtgtcgcag caggtggaag
     3121 agcgcgccta cgagctccag ctgcgcaacc ggaaagatga gccggtgacc gtgcacgtgg
     3181 agaaggcgct tcccggcgac tggcaggtga tcgccgccga tttccggtac gagaggaagg
     3241 atgcgcggac gttgacgttt gacgtcccgg ttccggcccg cgacacgacg acgctgaatt
     3301 acacagtgcg ggtgaagtgg tgacgcacag agcggttgac aagcccggtg cgagtctgta
     3361 gattcgccca gcgaatgagg aaggtctctg aatgaaccca ggaggtaccg ttttgaaaag
     3421 aacagctgtg atagtcatag tgatgctcgc ggccgcggcc ctgctggcgg gcagctcttt
     3481 gggcgggggc aaaccgctga aagcgatgat cagcggcgcc aagatcgcga tcatcgaggg
     3541 ccggccggat gaagcgctgc tcctgctcga cacggtggca atcagtttcg gggcggtgcc
     3601 ggaggcctac cagtggacgt tccgggtgta cgccgacaag atggagggcg cggcgggact
     3661 ggacgccaag cggccccacc tggtgaagat gctggcgtac atcgactcac tgcacctgac
     3721 ctgcgagggg aagattccgg tgcaggacaa gaagtatctg aaagactgcg cgaagtacat
     3781 ccagctggcc gactccacca aggtcaagta cttccggttc ttctataatc ttggtcacgg
     3841 tcaggtgaag acgctcgaac agctggccgg cagcctcgag ggggaaaccg actcgacgcg
     3901 tctggcctac atccgcgacc agatcaacac caacatcgac tccgtgatcg ccaacatgag
     3961 catggcgatc ctgatcgact cgagcgacta cagcccgtat gtggcgatcg gcgactccta
     4021 cgaccgggcg ggcaactacg agaaagcgat cgagtggatg acgcgcggtt acgagcgggc
     4081 gaagaacccg ctgctgctct cgcaacagct ggcgtactac catgtgcgtc aggatgacta
     4141 ctgcggggcg atcccgtatt tccaggagta cctgaaggcg tatcccgatt cgctgaacac
     4201 gatgaactac ctcgccgcct gctacaacaa ctgcggcctg gcgccggaga agcggatcta
     4261 tgtcgactcg gccatgacga tctaccgggt gatcctcgag cgggcgccgg agaacccgaa
     4321 cgtgtgggcg gccggcggcc ggtacttcct catcaaggcg caggacatgt ccgactcggc
     4381 ctcggcggcg cgcacccggc aggatgcggc cgcggccgcg cagttcgatc tcctgcggcg
     4441 cgccatgttc gattcctcgc ggacctattt ccggacagcc tgggagcagg cgcccgcgga
     4501 cgtggcgatc gccgagcagt ttgccttcac ctcggccctg ctggaggact gccccgcggc
     4561 ggtgaaggga ttcgaggtgg tcgcggaggc caaaccggac aacgcggaga actggatctc
     4621 gctgggcgac tgctatctgc ggatggcgga cttcccgaac gccgtgcggg cgtacgagaa
     4681 agtcgccgcg ctccagcccg gcaaactcaa gatatgggag aacctgggcg ccctctacaa
     4741 ggagaccggc caggcggaca aagcggccga ggcggccaag aaagtccagg agttgtcggc
     4801 ggccgggcag ggatgagccg gactccccgg cgaaccatcg gcaacgatgt tgtgagattc
     4861 acccacggca ggcttcggcc tgccgttttt cttccaccat ggcggcacga cacgcttcag
     4921 gcggaccgta cgtaaacgtg gcggtcgcgg gaccgatgca gcgaacgttc acttatgcgc
     4981 tgccgccggg gtcggcaccc ctctcccccg gccagcgcgt tctggtgccg ttcgggcgct
     5041 cccgcaccgt gggcttctac ctcgggccgg ccggccgccc tccagagggg atcgcggcca
     5101 agccgctcgg gcgggcgatc gatccctaca gctatttctc cgacgagttg tttgccctgt
     5161 gcgtgtggat ggcggagtac tacctggcga acccggccga ctgcctgagc gcagccctgc
     5221 cgccgacgat caagaaccgg gccgaggcgc acctggtgtg gacggaggcg gcggcggagg
     5281 ccgctccgga gctgcgcgac ctgatccggc cggggcggca actgcggccg tccgacctga
     5341 ccgcgcttcg gcggcggcgc ctcccggtgg cgcgcctggt ggccgccggc gccgtggtgg
     5401 agcgctggcc cgaggccaac gcccacaccg ggcagccggt ggtcggctac cgcgccgccc
     5461 gcctcccggc ctgggaggag tggtttgcgc gcgccaaggt caagccgtac ccgttcgacg
     5521 gcatgaagag ccgggccgaa ctcttggccg ccggctggac ggcgcactat gtggccaagg
     5581 cgatccgcga ggggctgttg gagccctgtt accgcgagga gaccgccgac ctctacgaca
     5641 tgatcgcacc gcgcgaaaac ctcgagcaga tccggctgac gcaccaacag gaggcgatct
     5701 tccggcaact ccgcccggcg gcggccaacc cggccttcgg cgtccacctc ctccacgggg
     5761 tgaccggctc gggcaagacg attgtgtact gccacctggc gcgcgaggtg gtcgcggccg
     5821 ggcggacggt gctcgtgctc acgccggaga ttgcactcac gggcacgacc ctggcgtact
     5881 ttcgtggatt tttcggcgac cgggtgaccg tcattcactc ggcgatgagc gaccgcgagc
     5941 ggctggccag cttcaacggg atccgtgccg gcacataccc gatcgtgatc gggccgcgct
     6001 cggccatctt tgcgccgctt gcgcatctcg gcctggtgat tgtcgacgag gaacacgatc
     6061 cgagctacaa gcaggacgat ccggcgcccc gcttccacgg gcgggactgc gcgatcatgc
     6121 gcgggcggat caaggatgtc ccggtgctgc tcggctccgc ctccccctcg atcgaatcgt
     6181 actaccaggc gcagaccggg aagtaccggc tgctggaatt gaccgagcgg ccggccggag
     6241 cgacgctgcc ggcggtgcgg gtgatcgaca tgaaaaccga ccgcctgggc ggcgacctcc
     6301 ccttcctctc ctactcgctc aagaaggagg ttgacgagcg gctggcccgc gacgagcagg
     6361 tgatcctgtt tctcaaccgg cgcggctact cgccgcagct gaagtgcagc cgctgcgggc
     6421 gggtgccggg ctgcccgaac tgccaggtga aactgaccta tcataaggag gggcggaaac
     6481 tgtcctgcca ctactgcggg tgggtggagc agcgctacga cgcctgcccc tcgtgcggcg
     6541 acccgcactt ctttccggtc ggcgcgggaa cccagcgggt ggaagaaacg atcccgcggc
     6601 tattcgagag agcggcggcg ctccggctgg attccgattc cgcaaccggc aggcggcgac
     6661 tgcacgagat tttgacggac ttttcagcag gcaaagcgaa tctgctgctg gggacgcaga
     6721 tggtgacgaa agggctcgac ctgccgggcg tgacgctggt cggggtgctg gcggccgacg
     6781 ccctgctgga cctccccgac ttccgggcct cggagcgggc gtttgcgcaa ctgctgcagg
     6841 tggccggacg ctcggggcgg gcggccaagc cgggtgaggt gctcattcag acctacgacc
     6901 cggagcaccc ggtgatcgac cgcgccgcgg cccaggatta ccgggggttt tatgagtccg
     6961 aaatccgcaa gcgggaggaa ctcggctatc ccccctactc ccggctggtc aatttcgtgc
     7021 tgtcgggcga cgacgccaag cggctcgagg aggcggcgct cgcattccgg gaggaactcg
     7081 atgcacgcat gcgcgaggcc ggggtgaacg ggcgggtgct cggtccggcc ccctgccccc
     7141 tgtactacct gcggtcgacc taccgccggc acctctttgt caagacgcgg cagatccggg
     7201 cgctggtggt gatgctgtcg gcctgggagc agcagaccgc gcggttcggg ctgccggcga
     7261 agatcaaggt tgtcgtggat gtcgatccga acgatatgat gtagccaggc cggggcggcc
     7321 aagacagaaa aaccgccggt ccggccatgc ctttcgttcc cctcaaagca ctcgtagagc
     7381 cgactccggc ggttcttccg actgtgacac tgtggctctc tcggaagaag gacgcacaaa
     7441 gaggtctcct ctttgcagag cagccggaat tcccttccga ctggtccgcg tgaggaagcg
     7501 cgggcgggct tccatatccg cccgtcgcat gcgtatgtaa caagttttgt gccggatcgt
     7561 ttccgcaatt cccaacattc ggcgatcgca cagtacggca agaacttaac caattgatgg
     7621 cttcggcggg cattcgcgcg gacgcacgaa tcacccctaa aatcggccgg tttggtcgtg
     7681 agaaaaaacg aattgccgtt ccggggagtg gtcgcgggcg ccatcgccgg acagcagaaa
     7741 gccgccgggg tgaaccggcg gctttcgaga acgaactgcc acttgatggc tgatggtgat
     7801 cgtgtggaac gaaggagggg ctcagtcttt tcccttgtcc ttctccttgc ccttctcctt
     7861 ctccttgccc ttttccttct ctttgtcctt gtccttggcc ccctcggcgc cctcttctcc
     7921 ctcggctgcc ggggcggcct cgccctcagc cgccaccgcc gcttccgccg cctcggccgc
     7981 caccgccttg atgacagtcg gcgcgctgat cacgacgacg gtgcgatcgg attcggcgag
     8041 gatttcgacg ttggggatag tgaggtcgga gacgtgaatg gcgtcgccga tgcccaggtt
     8101 tgagacgtcg acgttgacca cctcggggat attcttgggc aggcaggaga cctcgatgtc
     8161 gcgcatcgtg atctgcatga tgccgccttc atctttcacg cccttggggg tgccgacgaa
     8221 ggtcagggga atatcgatgt tgaggggttt ggactcgctg atggcgtaga agtcgacatg
     8281 ggtaatccgg ctgctgaccg gatcgcgctg gacctcgcgc aggatgaccc ggttggtaac
     8341 gccctcgacg gcgaggttga agatggtggt tccggagccg gccctgaggg tggcgtggaa
     8401 ttcccgctcg ccgagagcaa tagtccggga ctcgacctcg gggccgtaga cgacggccgg
     8461 gatttcgccg gccatgcggg tccgccgggc gggacctttc ccgaaccggt cgcgggggcg
     8521 ggcggcgata ggtacttctt tcatggcgca aacacgtcct tcgttgagac tataaacaac
     8581 atttcatttc ggtcacacga cgagcggctg gtcctcaaag agagaggaga cggattcctc
     8641 gtcgaagatg cgcttggtgg cctccccgat cagctcggag caggtgagga tttcgaattt
     8701 atcggtgagc ggcttgccgc tgtggtcgat ggaatcggtg atgatcatgg tcttgatggg
     8761 ggacgcgatg atgcgggtga tggcgttgcc cgagagcaca ccgtgggtgg cggcggcgta
     8821 gatgtcgcgg gcgccgcgtt ctttcaggaa atgggccgcc tggcagatgg tgccggcggt
     8881 gtccaccatg tcgtcgcgga tcaggatgtt tttcccctcg acgttgccga taaggttcat
     8941 gatctgcgcc tggttggggc gggggcggcg cttgtcgacg atggcgagtt cggcattgag
     9001 ggctttggcg gtggcgcggg ccagcttgat cgaaccgaca tcgggggaca ccaccaccag
     9061 cgggtcgagg ttgagggtgc ggaagtactt gttgaagatg ccggaggagt acatgtggtc
     9121 gtgggggagg tcgaagaagc cctggatctg gctggcgtgg aggtccatgg tgatgacgcg
     9181 gtcggcgccc acggtggtga tgaggttggc gataagcttg gcggtgatgg cgacgcgggg
     9241 gcggtccttg cggtcggcgc gggcgtagcc gtagtagggg atgacggcgg tgatgcgcat
     9301 ggccgaggcc cggcgggcgg cctcgatgag catgaggagc tccatgatgt tctccgccgg
     9361 ggggttggtg gactgaatga cgaacacgtc ggcgccccgg atgttgtcgt tgatctggac
     9421 aaacacctcg ccgtcggaaa accgggtgac ggtgcagtcg gtgagcttct cgcccaggta
     9481 ggcggcaatg cgttcggcga gcgggcggtt ggcattgccc gtcaccagtc gaatttcctc
     9541 gcgaactatc atgggtatgg accttgtcgt tacaccggaa agttaaacct gtttcggcgt
     9601 ggctgcccgg ccgcgtcgaa gcaggctctc accaccatta tctcgttgtc tggcacggta
     9661 tataactggg gcgccaggat tcgaacctgg gaatgccagc tccaaaggct ggtgtcttac
     9721 cgcttgacga cgccccaaaa gcgtcttgat gccgttaatc gcggcggttg gggccggcgc
     9781 cggggcgttt cagaccctgg cgtgggcttt cagctcctct tcataggccg ccaacacccg
     9841 ttcttcaatc gccagccgca tttcggcgtt gaccgggtgg gcgatgtcct gatgcgttcc
     9901 gttggggcgc cggcgggagg gcatggccac gaagaagccg gtgttgccct gaatgatctt
     9961 catcccgcgc accacgaacg cgttgtcgaa cgtcacgttg gcgaaacccc ggaggcgttc
    10021 ctcttctcgc aacgtcactc gtacttcggt gatttccacg gtgcctccaa tttctcagct
    10081 ctcctgcgac aacagggtga ttggcgcaac cgcgtaacac cgccaatttc cccgtctctg
    10141 tacaaccaga tcctggtgtt ccggctctga tgcaaacagt ccgaacacgg tcggacccga
    10201 tccgctcatc cgggccagga cagctccgcg tttcagcagt tcaccggcga tctcgtccaa
    10261 ctccgggtgc gaccggcggt gaatcgattc gaaatcgttg tctgcttgac ccaagtggtg
    10321 gacatactca tccaccgccc ggcaacaggg caagttaaac gggaccccgg aatctgtcaa
    10381 gttccttttg agggccgcat aggccgtcgc ggtggacagc gggaaccccg ggttgaccag
    10441 gagcaacccg tagtcggtcg gaaattccgt ctcctccatt aactctcccc gaccgcggac
    10501 aagcgcctga ccgcgcgtga agaagaaagg aacatcggag cccaagagca gccccagacg
    10561 catccggtca ggtggcgcaa gccgcagtcc aaatagatgg ttgatcgcct cgatggttgc
    10621 agccgcatcg ctcgatccgc cgccgagccc ggccgcgacc ggaatccgtt tctccagaac
    10681 tatgtgaagc ccctgattca gaccgaactc ccgggccatc aggcgggcgg cccgaacgac
    10741 caggttgtcc tcgccggcgg gaaggccgtc ctccccgatc acgtccagcc gcacgcccgg
    10801 agcatcggcg agcgcgaccg tgagagtgtc gcgcagcgag accgcctgaa acagcgagtt
    10861 gatgttgtgg tagccgtcgg gccggcggtt gagcacttgc aggaagaggt tgatcttcgc
    10921 cggtgcctca acgcgcactt tgccaggcgc tatctgtata gtattcatat tggcacagaa
    10981 aaagacttcg tccttggccc acccgtaacc atatcgccct aatttaagat aggcaattgc
    11041 gggcgcgtgt caagggggtg aggccaaata ggacgcaaga gaccgcgagg cgagcgcatc
    11101 cggctgcggc gcaaggagat agggtggggc ggcaaacggg atcgacaagc gccgtcaatg
    11161 aaaccgacgg gaggtcgcgg agaaacgcct gcgggaggcc ggaggatgaa tgcttgctgc
    11221 cgtatagaaa cggagaactc cggagttcat accgcgtcgc agcgtgaatc ccggtctcaa
    11281 gcacttccga cgcacggagc gcgccggggc gctgtcttac gatagcgccg gtgcgtcatc
    11341 tccccgtggt ctgctcctcc ccttgtcctt tctcgagaga gggggaattt cgctgcgcat
    11401 ttccggcttc gagccatcgg agagccgccg gcgagttgca ctgctgccac tcggcgatga
    11461 agacatcgag gaattccgac aacctctcct tggcgatgtc cttgaaatcg tcctcgccgg
    11521 cgaggccgaa ggtgctgcgc tcccagaagg aacaatagac cggcggaagg ccccggagct
    11581 tgaccccggt ccgggatttc tcgaaccggg cgtagtccag atagtcgcgc gcgctcaggg
    11641 tgaggacgta cgtgacgagg ccggtcggag aaccgtcgtc gaggcggttg cggatgaagt
    11701 cgatggagta gaccaggtcg gggatgttca tgtgcatgct gtcccggtcg aggcggccgg
    11761 gagcgtcgta caccgccagg cggtggtgcg ccaaggtgag ccgcatctgg gcgcgaaaga
    11821 gctcttccgt accgaacgca taaagcgcgc ccggcgacat gcacactgcg accgtgacgc
    11881 cgtcaagtcc gagcagcgcg gtgcgaggct cctcggcggc gggcggcacg tgaaccggaa
    11941 aggaatcgcg gccgacacct ttcgcatcca ggacgagcag acagagcagg ctgtaaatcg
    12001 cgcaatgaac gtggatcatc gctcttcctc cctcggtgca gcaaggctgc tgtttttgga
    12061 cgattaacgt ggcgctctgg gcccacaata cgctatcgaa tatagccgcc ggccgcgccg
    12121 acacaatctt tttcttctct gcttcgcctt tcgcgtttcc tctctccccc ttgcgttccc
    12181 ccgcattcgt tcatatcttc atcccctgac ccggacgccg ggcggcggca aggcgtagcc
    12241 cgacgttcga taagtgcgtg aaacgagtga agttacccat acaggaggat cgcatggacc
    12301 cgtttggcgt caaaggcaag ctcaaagccg gggggaacag ctacaccgtg tatcgcctgg
    12361 acaagctggc cgggcagcgg ggaatcgacc gggcggcgat cgagcggatg ccctactcga
    12421 tcaaggtgct cctcgaagcg gtgctgcgca acgccgacgg ccgggtgatt cgggacgagg
    12481 atgtggcggc gatggcgcgc tacgacgcca ggaaagcggc cgagcagagc cacgagttcc
    12541 ccttcaaacc ggcccgcgta ctccttcagg atttcaccgg ggtgccggcg gtggtcgacc
    12601 tggcggcgct gcggtcggcg atgaaacgga tgggggggga cccgaagaag atcaatccgc
    12661 tcgtgccggt tgatctcgtg atcgaccatt cggtcatggt cgaccggtac ggttcggacg
    12721 acgcgctggc ggccaacatg gagatggaat ttcagcgcaa cgccgagcgg tacgagttcc
    12781 tccactgggg gcagaaggcc tttgcgaatt tccgggtggt gccgccggcg accgggatct
    12841 gccaccaggt gaatctcgag tacctcgcca agtgcgtgct ggtgcgcgac ggcgaggcct
    12901 tcccggacag cctggtcggg acggacagcc acacggtgat gatcaacggg ctgggggtgg
    12961 tcggttgggg cgtcgggggg atcgaggcgg aggcggtcat gctcgggcag ccgctctata
    13021 tgctgacgcc ggaggtgatc gggttcaagc tcacggggaa gctcgcggag gggacgaccg
    13081 ggacggacct cgtgctcacg gtgacgcaga tgctccggca gaaaggggtg gtggggaagt
    13141 ttgtcgaatt ctacggcgag gcgctcgaca cgctaccgct gccggcaaag gcgatggtgg
    13201 cgaacatggc ccccgaatac ggcgcgacga tggggttttt cccggtcgac gagctcacgc
    13261 tggactacct gcgcatgacc gggcggaccg acggcgaggt cgacctggtg gagtgctact
    13321 gcaaggagca ggggctgttc cgcgcgaagg gagcggcggc ccccgcgttc acggacacgc
    13381 tcgagctcga tatctcgcag gtggaaccct cgctggccgg tcccaagcgg ccgcaggacc
    13441 gggtggcgct gtcgaagatg aaagacgaat tccagagagc gctcgcgcgg ccgatcaaag
    13501 agcgcgggtt cgagctcaaa ccgagcgaaa ccgagaggca ggtcaagatc agcgacgggt
    13561 accgggccgc gatcgggcac ggcgcggtgg tgatcgccgc catcaccagc tgcaccaaca
    13621 cctccgatcc ctcggtgctc ctcggggcgg ggctgctggc gaagaaggcg gccgcccggg
    13681 ggctgacgag caaaccgtgg gtgaaaacct cgctcgcccc ggggtcgcgg gtggtgatgg
    13741 attacctcga gaaggcggga ttgatcgagc cgctgaagaa gctcgggttc cacaatgtcg
    13801 ggttcgggtg cacgacctgt atcggcaaca gcgggccgct gcccgaagcg gtggtcaaag
    13861 cgatcgagga ggggaacctg gtggtgtcgg cggtgctgtc gggcaaccgg aatttcgagg
    13921 ggcgggtgaa cccgcacacg cgggccaact acctcgcctc gcccccgctg gtggtggcct
    13981 acgcgatcgc cggaacgacc gcgatcgatc tggcgacgga gccgatcggg accgatgagg
    14041 gcggaaagcc ggtgtacctg cgggacatct ggcccagcca gcgggagatc gccgaggcca
    14101 tggcgggagc gatcagcccg gccatgttcc gcgcccgcta cggggccgtg ttcacgggca
    14161 acgagcggtg gaaccggatc cagtcgcccg cgagcgagct ctaccggtgg gacgagaaat
    14221 cgacctatat ccaggagccg ccgttcttcg tcgacatgaa gccggagccg ggggatgtcg
    14281 agccgatccg ggacgcgagg gtgctgggga tattcggcga ttcgatcacc accgaccaca
    14341 tcagtccggc ggggtcgatc aaggccgaca gcccggcggg ccaatacctc ctctcacacg
    14401 gcgtgacttt tgccgacttc aattcctacg gctcgcggcg cggcaatgac cgggtcatga
    14461 cgcgcggcac cttcgccaac atccgcatcc gcaacctgct cgcccccgga accgaggggg
    14521 gcgtgacgat ctatctcccc gccaaccagc agatgagcat ctacgatgcg gcgatgaagt
    14581 accaggcgga cggaacgccg ctgctggtga tcgccgggaa ggactacggg atggggtcct
    14641 cgcgcgactg ggcggccaag gggacgagcc tgctcgggat cagagcggtg ctcgccgaga
    14701 gtttcgagcg gatccaccgc tccaacctgg tcgggatggg cgtcctcccg ctccagttcc
    14761 tgccgggcga agcgcgcgag aggctggggc tgaccggcca cgaactgtac acgatcgagg
    14821 gactctcgaa cgacatgaag ccgaaacagc gggtgacggt gaaggcgctt tcgaagaacg
    14881 gcgagaaacg gttcgaggcc gtcgcccggc tcgacacgcc gatcgatgtc gagtactacc
    14941 ggcacggggg gattttgcag atggtgctgc ggcagctcgc ccgggcgtag gcgaaggcct
    15001 ccccgggccg gccggaggaa ccgtcagcgg ggggcgagca actcgatctg cgagagcacg
    15061 gcggtgacgg cgtgctcgac gcggagggtg aagcgcccca agctgaaggc gcggaaaccg
    15121 agcgaggtca gcagctcgac ctcgaacggc acccatcccc cttccggccc gacagccgcc
    15181 aggagacggg gcgaatcgcc gcgatagacc gcgtcgagca tcgccgctgc gccggggtgg
    15241 ggcgcgagcc gcagcgacgg ggcggattcg ttctgctcga gcaccgggag gatatcctca
    15301 aagaagcgcc ggaaccggtc gtgcacggcg acctcgggca ggcgggtcag acgtccctgg
    15361 ctgagcccct cgatcaggtg cggcgtgtac tgctccgggt tcaacagcgg ggaggagaag
    15421 tagctccttt cgacgcgccg ggcgcggacc aggtgaaggc ggcggacccc catcatggcg
    15481 gcggcgaaca ggattttctt gagagtctgc gggcgcggga gagcgcagat gagatcgacg
    15541 gtcggcacgg gggccggcgg aggggcggtc caccggcagt ccaggagggc ttcatcggcg
    15601 gcgaggcgcg ccacgcgggc ggtcccgcac gggccgttaa gaaggccgac ctgcacgagg
    15661 tccccctcgg ttgcgcgcag gacggtgcgg aggtggtcgg cccgctgcgc ggccagtcgg
    15721 aaggtctcgc cgccgacgcg gtcgtgttcg gtgaggatga tgaggttcat ggcatcgtgc
    15781 gcgccggagc gcgacctttc cggacgatta cggttgcggg cggcgatcat accgggcagg
    15841 ggaaggatag ggtcacgagg gcgcgaagtc aactccggcg cggcgcccgc cgggccgccg
    15901 caaagtagat attgacattt ccggcggtca ttctacatta ggcagacatg atgtaattac
    15961 ctgcctgcaa gcgggtctaa ggaaagaagt ctgccatgtc ggaagctgtc aagaccgcat
    16021 tctacgatta ccatattcag gccggggcga agatggtcga attcgccggg ttctgcatgc
    16081 cgatccagta ctcggggatc accgacgagc acctcgcggt gcgcaccaat gtcggactgt
    16141 tcgatctgtc gcacatgggg gagttcgagg tcagcgggcc ggacgccctg gctttcctcc
    16201 agagaaccac gaccaacaac gtcgccgacc tgcaggtcgg ccagatccag tactcgtgca
    16261 tgacgatgga gcacgggggg attgtcgacg acctcctggt gtaccgaatg ggcccgcagg
    16321 agtacctgct ggtggttaac gcctcgaaca tcgccaagga tttcgcgtgg ctccagtcgc
    16381 acctggcggg caatgtccgc ctggtcgacc aatcccccgc cacgagcctg ctggcggtgc
    16441 agggaccgaa ggccgaagag atgatgcggc aggtgacgtc gttcgacctg ggcgcgatgc
    16501 cctactacac ctgggcgcgg gccgagatcg gcggagtgaa ggtgctgttc tcgcgcaccg
    16561 ggtacacggg ggaggacggt ttcgagctgt acattccgcc gccggacacc gaccggctgt
    16621 ggcgggtgct gacggaggcg gggaagaaat tcgggatgaa actgatcggt ctgggggcgc
    16681 gcgattcgct ccggctggaa atgaaaatgg ccctgtacgg caacgatatc gacgacacga
    16741 cgacgccggt ggaagccggg ctgtcgtgga tcgtccattt cgacaaggag ttcatcggca
    16801 agccggtgct cgagaagcag cgggatgaga agccggtccg ccggctggtg tgcctggagc
    16861 tcgagggcaa ggcgtttccg cgccacggct acacgatcac cgacggggcg aagccgatcg
    16921 ggcaggtgac ctcgggcacg ttctctccca gtctgcagaa agcgatcgcg atggggtatg
    16981 tcccgcgcga cctgagcaaa gtgggatcga cggtgagagt cgcgatccgc ggcaaggagt
    17041 tcccggcggt ggtggtgaag ccgccgttct ataagcatgg ctcgcacaag tagactccag
    17101 atgcaggtgg acctgtacct gacgccgatc ccgttcgggc gggcgaagct gtcgggtcgg
    17161 acggtggtgg cgatcgacgt tctgcggtcg tccacctcga cctgcgcggc gctcctggcc
    17221 ggggcgaagg gagtgatccc gacgcccggc cccggcgagg cgggcgaact ctgggccaag
    17281 ctgggggggg acatggccgt gctggcgggg gagcgggaca acgtgcggat cgagaatttc
    17341 aagctcggca attcgcccgc ggagttcacg ccggagacgg tcggcggcaa attcgtgatc
    17401 atgacgacga cgaacgggac gggcgtgtac cgggacgcgg tgggagcgga cctggtgctc
    17461 tgctgcgcgc tggtcaatgt cagcgccgtg gcccgcaagg cggccgcggc cgggcacggc
    17521 ctcgtgctcg tctgctcggg gcaggagggc gggttttcga tcgaggatac gatctgcgcc
    17581 ggcatgctcg tgcacctgct ggccaccgaa cacaaactga atctcgcgct gaacgacgcc
    17641 gggtcgctgg ccctcctgtt gtaccgctcg aacaagagcg ccctgaagaa gaccattcag
    17701 cagggggagc acggccgccg actgaccgaa ctggggttcg gggcggacgt ggagacggcg
    17761 gccgcggtcg acaccatgcc agtgctcccg gtcatggtcg acggccgcct ggtggcggac
    17821 gtggatccgc ccgcggctcc gccggccgag ccggcgtgag ttcatcgcca acccttcccg
    17881 agagtataag aaagcatgag acacctgatc attctcgcgg ccctggtcag cctggaggca
    17941 ggcgccccgg cggccgccgc ggatgccgtg cggttcgccc tcgacgttca ccagcaggac
    18001 tcggcggccg gagccgtcct gctgtactcc gacacggcga ccgtggtgcg cggggtcgcg
    18061 gcggccgggt ttgcgctggc gttttcggca gaagtggaag tgacggcggc ggacaccgcc
    18121 cacgcccagt ttctcgtgca cgcggtgacg ctcggaccat cggccagaac ctacgcccgc
    18181 agttattcgg ccgagtacgg tctccccgcc cgcatcgagg ggatcgaggg gaagggaggc
    18241 gggcggctga tgctcacggt gacaccgctg gcgttcacgg atttcgacac cgcccagtgc
    18301 ctcttcgacc accgggtcac cggggcctac aagattcagc cgagcgccag cctcgacgtg
    18361 cactacgtcc cgaactcgtt cggcgattac tatatcgagg ctttcaaggg cgtcctcgaa
    18421 caccactacc ggcagtttca ggaagtgttc cagttctcgc tgccgggcaa gttcagcatc
    18481 ttccttctcc cctgcgcctc gccggcggtg ctgtgggaca cgcggtacgg gcaggtgacc
    18541 gatccgacgc ggagcacggc gttcgcgatc gcggccaacg gtcagaacac ggtcgatccg
    18601 ttcatcgcca cgcacacggc cctcctgcgg agctggggat acgccccgcc gttcttgtcg
    18661 gagggggcgg cgaactacac ctcgttcgcc ctgttcgaca tgaagaagct tgcggccgag
    18721 ggccgggtgc cgccgctgga cagcctgctg gacacttacg cctacctgac cgcggcgccg
    18781 ctggtagcgg accgcgcctc ggcgaccttc gcccagtacc ttatcgacag ctacgggctg
    18841 gaggcgtttc gcgcgttgta tgtgtcggcg gacgatctca acctccgccg ccgcctggcg
    18901 ctggcatacg gcaaggggat cggggcgctc gaggccgagt ggcgcgctta tgtcgacacg
    18961 tcgacaattc gctttcccgc actcgcccag gaggcggaca aggcggaggc cctgttccag
    19021 tacgggcgga tgcgggacta cgcgcgggcg atgctggaat tggcgccggg gcggatcgat
    19081 tcggcctttg cctggtcgcg gctgaaacag gccgagttca tgctcggcaa ctactacggg
    19141 gcggccgatg cggccgaagc gcaggtgcag cttgacggcg agcgggcggt caactggatg
    19201 acgctcgggt cgttccaaat gatgaacggg atgtacgccg aggcccgcgc gagtcttcag
    19261 aacggccagt cgaaggaccc ctcggatcaa accatcgcgt tcaaccgggc gctcgcggcg
    19321 ctctacacgt ccgacaccgc gaccgcggaa tcgctcctgg tcgagatcat cacccgcccg
    19381 gaaagccgcg cgccgcaggg ggaggcccgg gtggtgctgg gcgagattct cctttcgcgg
    19441 ggcgacaagc gcggccggga ccgcgccgcc acctatttcg tcgaggccat gcagacgttc
    19501 cagcagcagc tctcggtcaa ccggacgatg ccgacggcct acctctggct gggggcggcg
    19561 gcgctcggca tgggggacac cgagctggcg cgggacgcgc tggagaccgg gttgttcctg
    19621 gaatcgcggc cgttctacct ggggcagatg aacctgtggc tgggcaagac ggccgacgtc
    19681 ctgggggacc acggccttgc gcaggagtac tacgggcagg tgctcgccgg ctcgtcggcc
    19741 gcgtacgcgc agcaggaggc ccgccgctgc cttgagaaac cttaccgcat aaagtagcag
    19801 agcgtgttca actttctcaa ttcgggcgtg ctgttcgccg cgctcgcggc gctgattccg
    19861 cttctgattc acctcttttc gaagcggcgg gtgaagatcg tcgagttttc gtcgctgaag
    19921 catctcaagg agatgcagcg gcggcaggtg cggcggctaa agatccgcca gattctcttg
    19981 ctgctgttgc ggatggcgat catcctcgcc ctcgtgctgg cc
//
LOCUS       DAOPOE010000059        19871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3354_length_19871_cov_4.913000, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000059 DAOPOE010000000
VERSION     DAOPOE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19871
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_19871_cov_4.913000"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="corA"
                     /locus_tag="PKM94_08870"
     CDS             <1..1059
                     /gene="corA"
                     /locus_tag="PKM94_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOD66788.1"
                     /translation="HSKKAGTRPGTVAYVGRKREHSVAIDVIEYDAHHIAERRVHSLP
                     ECLPLKPAPAVTWLRINGIHDPRLIEQIGAHFNIHPLTQEDIANTAVRPVLEESPALV
                     SIILKRLSLDGGGLLHPEQAAVIFSRHLVISFQERDSGPFDAVRDRLRSTVPRTRFLE
                     TDYLAYSLADALIDDYFVTMDRLGEQLDAAQDELVRRPTPDHLETIFALRRELLLFRK
                     ALWPLREVIAALARSENRLIHDDTRPYIRDLYEHTVQALDTIETMRDMAASLLEIYLS
                     SVSNRMNEVMKVLTIIGTVFLPLGFLAGVYGMNFDRTAGRLNMPELGFPYGYIVFWVL
                     VLLVVAAFVLFFRRRKWI"
     repeat_region   1112..1739
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1178..1213
                     /rpt_unit_seq="gctgggacttgacaggaaccgccgcttaagtacatt"
     gene            complement(1792..2097)
                     /gene="cas2"
                     /locus_tag="PKM94_08875"
     CDS             complement(1792..2097)
                     /gene="cas2"
                     /locus_tag="PKM94_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOD66789.1"
                     /translation="MWVLAMFDLPVDSNVARRRYAQFRMHLLKEGFLMLQLSVYGRYC
                     GSEERANICRKRIREALPPEGEVRVLAITDRQYGKMEVFTGTKRHRPEQPPAQLMLF"
     gene            complement(2139..3026)
                     /gene="cas1"
                     /locus_tag="PKM94_08880"
     CDS             complement(2139..3026)
                     /gene="cas1"
                     /locus_tag="PKM94_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOD66790.1"
                     /translation="MGERFLDISEQPARLSIRHRQLVIGVDGSEQTTPLNDVSALVVS
                     NPAVSYTQGVLAGICEAGGALVVCDEKHLPIGMLVSLLGHTTQGERLTAQAEAPPTLK
                     KRLWQQIVRSKVQAQGACLRRVRGEDFGLFRLARDVRSGDTSNIEGQAARRYWPALFG
                     TSFHRDRNRADQNRLLNYGYAVLRAMAARAICGAGLHPSLGLHHHNRYDAYCLADDLM
                     EPYRPAVDRVVLGMCDTRGPEGTLDAAAKRQLIGGIIGTRHAVENETAVLPDILAHAA
                     ASLAQVYLGRRRGLCLPVV"
     gene            complement(3082..5847)
                     /gene="cas9"
                     /locus_tag="PKM94_08885"
     CDS             complement(3082..5847)
                     /gene="cas9"
                     /locus_tag="PKM94_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOD66791.1"
                     /translation="MESPYQLRRRGISERLEPYEFGRALYHLAQRRGFLSNRRSAPKD
                     EDEAGKVRQGILDLEREMASVGARTIGEYFASIEGGGTRIRDRYTGRHMYEQEFDALW
                     NTQAGFAPDVLLPDLREKIREIIFYQRPLKSQKRFVGKCELEPKYRRASWEHLSAQRF
                     RYLQTVNNLAVIDKSTGEVRALSSEEREAIIERLEATAEATYSSFRGRGGIPSLRGTV
                     FNLERGGEKRIKGNRTAAQLAGVFGLERWRDLTEADREAVVEDVRSIVNDGALARRGQ
                     RKWGLAPEQAKRLTEIRLEPGHCAFSRQALDKLLPHLRQGAKLYDAIEAVPEYRERMN
                     RLTEAVDELPSVLSALGDIRNPLVIRSLTELRRVVNAVVQRYGKPDFVRVELARDLRQ
                     TAKVRLELSKKMRQNEQARMRAAERIRKETGIQNPSRDDILKILLAEECSWRCPYTAE
                     PFGIHDLLGRSPQFDIEHIIPFSRCLDDSFMNKTLCHADENRKVKHSRTPWEAYGRTE
                     RWEEIMGRVREFKGPGRWAKVRRFEMREAEHNAFINEFCSRQLNDTRYASKLAMRYLS
                     MLYGGGRTQGVDAEGKRRIQASAGMITALVRHAHGLNGLLSRDDLKSRDDHRHHAVDA
                     VAVALSDTRTVMRLTAAARRAEAYYKLFKDVEEPWPGFIGDIKAVIDRVIPSRRLSRR
                     VRGALHQDTFYGARTVDGRAVVHIRKPLAALHKGDIDDIVDPVVRAAVRRRVEEAGGD
                     VKKAFAADVEKPYLSGGGGIRIPIRRVRLAKNLVTFPVGSAHRRRHVTSDANHHITLF
                     EVCAEGGGAVRWEGKVVSLFEATQRLRRGEPVVRRDDCNGGRFLFSLSCGDVIELDRE
                     GTGRALYVVRTVPQSKQIRFVPIHDARRLKEIPKSGMTAVPDSLRRRNCRKVVVTPLG
                     EVRRAAD"
     gene            complement(6357..6944)
                     /locus_tag="PKM94_08890"
     CDS             complement(6357..6944)
                     /locus_tag="PKM94_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOD66792.1"
                     /translation="MRYITNYSSGKMGYAIAAAAAEQGASVTLISGPVELAAPEGVVL
                     VRVESTAELHRAVGERFAQCDCLVMAAAPADYRPSRISSEKIKRIKNLRLELEATGDI
                     LKDVAAGKRAGQIVVGFALETTDGVANARRKLREKKLDLIVLNEPSADSGFLTDTNRV
                     QVIGRDGEAEEWPLLAKGEVARRLVARVAAMLGKR"
     gene            complement(6998..7573)
                     /locus_tag="PKM94_08895"
     CDS             complement(6998..7573)
                     /locus_tag="PKM94_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOD66793.1"
                     /translation="MSLTNKKILVGLTGGIACYKVPYLVRALVKAGAEVQVVMTRAAT
                     EFITPLTMETVSARPVAVEMFAHGEYVATRHIDLAHWPDLIVVAPTTANFIGKAASGI
                     SDDLLTTVVCASGKPIMIAPAMNPQMWRNPVTQRNLKTLLSLGYRVVEPAEGEMACED
                     YGVGRMAEPEVILRAIEGFFAAPKGRAKKKA"
     gene            complement(7577..8050)
                     /locus_tag="PKM94_08900"
     CDS             complement(7577..8050)
                     /locus_tag="PKM94_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HOD66794.1"
                     /translation="MRNRILEIVVFLIDFMQGNKGNLAGSEDLSTVLEAEGYSQDEIS
                     SAYSWLLQRFDNTPEQYFSEIPLMNASTRILTPMERMQLEPEAQGFLLKLVNLSLVDH
                     EQFEGIMERVSVFGSKSVSLEQIKLIASSVVLADLEDFDELSLGEASPDRSLHVN"
     gene            complement(8285..8476)
                     /locus_tag="PKM94_08905"
     CDS             complement(8285..8476)
                     /locus_tag="PKM94_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66795.1"
                     /translation="MNALRRMKTLVLIFALVAIVTGGLVATFADNAQARPKCCIWVMY
                     CSEIPPYPCWDVCIPVPCP"
     gene            complement(8498..9106)
                     /locus_tag="PKM94_08910"
     CDS             complement(8498..9106)
                     /locus_tag="PKM94_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD66796.1"
                     /translation="MQFGHYRYAATVTILLAAALIAIGFLLVDNGRLRQMLRDPAAFV
                     AAQQTGSEVPPLQAADVTGGICRLSYGPGEPATLLCWLTMRSSRGKDNIPFWNELAHR
                     FAGRNVRVIGMCTGEPDEVRGYAAAQGVAFPVIAAGDPYIVEAYGGTVVPQTLLISPT
                     GQVAGRWPGVAGSTQQAEIEARAAEAGKLEQKTPETPENAIP"
     gene            complement(9344..10912)
                     /gene="purH"
                     /locus_tag="PKM94_08915"
     CDS             complement(9344..10912)
                     /gene="purH"
                     /locus_tag="PKM94_08915"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOD66797.1"
                     /translation="MEDTVKIKRALISVSDKTGITQLALELEKRGVEILSTGGTLQAL
                     KKAGAHAVSVSAFTGSPEILDGRVKTLHPRIHAGLLARRDVEAHLETMTQHEYKLIDL
                     VVVNLYPFEQTVAKAGATDDEVIENIDIGGPSMVRSAAKNHDGVAVVVDPGDYAELIA
                     EMNAHDGGTTRAFRRRCAAKAFALTARYDTAIAGYFAGAGQAGAKFAPQYRPSYSLRS
                     ALRYGENPHQEAALYARDGYGGPTLLEARILAGKELSYNNYGDLDSCLDMLLDFTEPF
                     ACVLKHANPCGAATGATIAEAYEAAYASDPLSAFGSIIGLNREVDLACARLLHETPFV
                     ECMIAPSFTDEALALLTKKKARRILALPSITLGWPRGQLADKPIRGGLLRQDTDDIET
                     NPDALTVVTKRAPTPEEIRSLLFAWKVVKHTKSNAIVLAKGTATVGIGMGQTSRVDSG
                     FMAVKRAGERARGSVMASDAFFPMPDGVEVGTDAGVTAIIQPGGSKGDDEAIAAADKA
                     GAAMVLTGHRHFKH"
     gene            complement(10966..11874)
                     /locus_tag="PKM94_08920"
     CDS             complement(10966..11874)
                     /locus_tag="PKM94_08920"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOD66798.1"
                     /translation="MAAETDRVVMQTEIREYPLMRRGKVRDIYDLGDTLLFVATDRIS
                     AFDVVMPNGIPGKGRVLTQMSLFWFDFLSDVVESHLVTADVDRYPAALRAYREQLEGR
                     SMIVAKADRVEAECIVRGYISGSMWKELQEARKRGTNAVHGFEFPPALGESEQLPRPI
                     FTPSTKNDDGHDINISYRELVGLVGAETAQRCRDSSLAIYTKAADYARTRGIIIADTK
                     FEFGYKDGRFMLIDEVLSPDSSRFWPVDTYAIGRGQPSFDKQPIRDYLDALDWNKKPP
                     APRLPEEVVQASAARYREAQRLLTGR"
     gene            complement(11864..12472)
                     /gene="purN"
                     /locus_tag="PKM94_08925"
     CDS             complement(11864..12472)
                     /gene="purN"
                     /locus_tag="PKM94_08925"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOD66799.1"
                     /translation="MADGRRPRIGVFLSGGGSNLQALIDASKCGILSGEIALVVSNLR
                     KAFGLERARQVQIPVFVYKEADYATPEEGQAALLRVLKEHGVEWIALAGYLKLLPGNI
                     LRAYPRRVVNIHPALLPKYGGKGMYGHFVHEAVLAAGEKESGLTIHLVDEAYDHGAVL
                     HQQRVPILPGDTPETLAARVLEQEHRWYARVLDKLIRGEYGG"
     gene            complement(12557..13420)
                     /locus_tag="PKM94_08930"
     CDS             complement(12557..13420)
                     /locus_tag="PKM94_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD66800.1"
                     /translation="MPYVSLGDFRLYYETSGPAASAAGRAPVVLLHGFTLDRRMWSET
                     REFLAREFPVIVPDARGHGRSDAPATGYSRADRVEDLARFADRLGLERFHLVGLSMGG
                     STALGYALRDQARLVSLTLVSTSAAGWNIGRKMERAEEQGREIRRRAHQEQGEDFAAA
                     AARGLAYVRERWIEYGLRVYREDQRSIRDRLEQMMREYSGAAWLDERRGKYPSPGNDV
                     ERVHTITAPTLIVAGEKDKVFAPLAQELGKRIAGSRVIIHRGVGHMVNMEIPEQFDGD
                     TGAFLRAAEKV"
     gene            complement(13607..14932)
                     /locus_tag="PKM94_08935"
     CDS             complement(13607..14932)
                     /locus_tag="PKM94_08935"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOD66801.1"
                     /translation="MTKTKQEILRLMDDGGVRYIRLCFTDILGRIKGMSIPRTEIEQV
                     LEEGQPFDGSSVEGFARIEESDLLAMPDPVTFRIMPWESSGERLAMMFCDIVLPDGTP
                     YDGDPRWVLRRQLQKVAERGWTFYVGPELEYFYLRDERTTDLIDVDGYFDMGTVDKGS
                     QVRKATVNALEAMEIPVECSHHEVAPSQHEIDLKYQEALVMADFAQVYKFVVKQVAMQ
                     HGLYATFMPKPVFGENGSGMHCHQSLFMGDKNLFANGTDQYGLSEIGRQYTAGILKYV
                     REFTLVTNQWVNSYKRLVPGYEAPVYISWGRRNRSSMVRVPAHRVGKERATRIELRSP
                     DPGANPYLAFAAMLAAGLQGIEQRLTLPDPVEENIYDMSEEQKARLNIEVLPNSLENA
                     VRMFEESQLMREVLGEHVFTKLIENKRIEWDQYRIHVSAFEVEKYLPVM"
     gene            15223..16602
                     /gene="selA"
                     /locus_tag="PKM94_08940"
     CDS             15223..16602
                     /gene="selA"
                     /locus_tag="PKM94_08940"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOD66802.1"
                     /translation="MTDKIISHPRDFPAVEELLQHPRLADLIARLPRPVAADVVRAVV
                     AEAKSRLTSQDRPLTRDDLLAALRAALRAAGRQAAARVINATGIVVHTNLGRAPLPAD
                     LFDAIKHSVTGYGNTEFSLETGRRGGRGGAAESYLALLAEAEAATVVNNCAAALFLAL
                     NSLAARKEVLISRGELVQIGGGFRIPDILRKSGARLREVGTTNITTIADYASNIDPKR
                     TGLILKVHKSNFTQAGFTEEVSLRDLVALGREHNLPVMNDLGSGLFIDTLPLLGYSEP
                     TVQQSVRAGAVLTTFSGDKLLGGVQAGLLVGRAEIIRTIKKNPLFRTMRVDKIVFSAL
                     ERLFSIYLGGTWQTDITLWRLLALPESELYKRGKKILADLGQPEDLSVEATQAFVGGG
                     ALPEAAIPSVAVCFSKTRNAAALLAAFRDRDLPIIGRIENDRFLLDLKAVETEDLPAL
                     TEAIRSVLG"
     gene            16609..18513
                     /gene="selB"
                     /locus_tag="PKM94_08945"
     CDS             16609..18513
                     /gene="selB"
                     /locus_tag="PKM94_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOD66803.1"
                     /translation="MIVVGTAGHIDHGKSALVKRLTGTDPDRLPEEKARGMTIDLGFA
                     FYRTPAGATVAFIDVPGHERFVKNMIAGAGGIDAVMLVIAADDGWMPQTEEHFQVVRL
                     LGVRHGLVVINKTDLVEPDWLELLTADIGARLAGTFLHDVPVFAVSAATGAGFDPLRE
                     YLNRLPGLVASRKDIGKARLFIDRSFVRPGIGGVVTGTLRGGSLRLGQTVGVWPARTV
                     GKIRSLHSGGDSVDLAEPGQRTAVSFTGVDREQLLRGGVITDRTDLEYFPHHPVLALS
                     LELLPNAPVALEDRRRVLLIVGTTEVEGEIRLFRAEEIRPGGSGLVFFQPDQPVYALI
                     GDRCIMRLPTPMVTLGGGQVLDHLRFFPRRRALDRYDYLLLRSSGRLEDLVRSELQKR
                     TAVHRPHLLEHADCAETAINQAVTRLIDSGQAALYRGYVFEPTAFAQALERFKRRLEK
                     HFERKSHLKGVPIETLQQLSPYGRETTDLLIQYALENRILVRHDDEYSVAGRGMTLAG
                     PVRTAYDEIMAALRADPWAPPKLADLTSRGKAHREAIRFILDSREGHKCGADFIFLAD
                     TWQTVLAYIRERLDRTGELAITDLRDRFGFTRKFAVPILEETDRLRLTRREGDLRVKG
                     ERYEVPLADL"
     gene            18488..>19871
                     /locus_tag="PKM94_08950"
     CDS             18488..>19871
                     /locus_tag="PKM94_08950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOD66804.1"
                     /translation="MKSRSLIYNANILTQDRRLAVDSMAIERGRIVAVGDNLRGVGPF
                     AGFDRIDLRRRTVVPGFVDAHTHFYYYALSLGRIDLHGVDSLDECLRLIRGFCRGLGP
                     DDWVVGEGYRPDLFAGRVEPDRRQLDRVVGRRPAFIFSHDQHSAWVSTRALELAGLTR
                     RTPDPAGGVIERDPDGSPRGLLREIPGYDPVFRLIPAPSRPLLDRLYGRALAQAYRRG
                     VTGVHSFDGPAGFAWFIERAARGALGLRINYYAPAPGLPELRAAGTRYGAGDDFLRVA
                     GIKIFADGSLGSRTALCYQPYRDDRRNRGVEVTPPGKIASLARAAARLGLPCAIHAIG
                     DRAVANVLDALAAAPPLRPPARHRIEHLQLVRRRDLPRLKKLNIVASVQPQQTVSDIE
                     MVRAAWGGRAKDAYIFRALLDLGIDLAFGSDVPIEPLDPLAGIAAAVRRAHGPGEAFP
                     DSGPAGKRARR"
BASE COUNT         3242 a         7403 c         5597 g         3629 t
ORIGIN      
        1 cactcgaaga aagccggcac ccgccccggc acggtcgcgt acgtcggccg caaacgcgaa
       61 cacagcgttg ccatcgacgt catcgaatac gacgcccacc acatcgccga gcgccgcgtc
      121 cactccctcc ccgagtgcct ccccctcaaa cccgcccccg ccgtcacctg gctccgcatc
      181 aacggcatcc acgacccccg cctcatcgag cagatcggcg cccacttcaa catccacccc
      241 ctgactcagg aagacatcgc caacaccgcg gttcggcccg tcctcgagga atcccccgct
      301 ctcgtctcga tcatcctcaa gcgcctctct ctcgacgggg gcggcctcct gcaccccgag
      361 caggcggcgg tcattttctc ccgccacctg gtgatctcgt ttcaggagcg cgacagcggc
      421 cctttcgacg cggtccgcga ccgcctcagg agcaccgttc cccgcacccg ctttctcgaa
      481 accgactatc tcgcctactc gctcgccgac gccctcatcg atgactattt cgttaccatg
      541 gaccgtctcg gcgagcagct cgatgccgcg caggatgagc tcgtccgccg tcccaccccc
      601 gaccacctcg agacgatttt cgcgctccgc cgcgaactgc tcctgttccg caaagccctc
      661 tggccgctcc gcgaggtgat cgcggccctg gcccggtcgg agaaccggct catccacgac
      721 gacacccgcc cgtacatccg cgacctctac gagcacactg tccaggccct cgacaccatc
      781 gagaccatgc gcgacatggc cgcgtcgctg ctggagattt acctgtcgag cgtgagcaac
      841 cgcatgaacg aggtgatgaa ggttctgacc attatcggga cagtcttcct gccgctcggt
      901 tttttggccg gcgtgtacgg gatgaatttc gaccgcacgg ccggccgcct gaacatgccc
      961 gagctgggct tcccctacgg ctacatcgtc ttctgggttc tcgtcctgct cgtcgtggcc
     1021 gctttcgttc tcttcttccg ccgccggaag tggatctagt ccggcctcgc cccgcccccc
     1081 gcacgcggct cctgtcaagt cttcgttggc tgctgggact tgacaggaac cgccgcttga
     1141 gtacatttca gcaccgacaa gccgcttgcg tatttgcgct gggacttgac aggaaccgcc
     1201 gcttaagtac attgtattct actgccgggc catcttctcg atggctggga cttgacagga
     1261 gccgccgctt aagtacattc ggcgccctga ttgtcaagac gccgccgcag ctgggacttg
     1321 acagaaaccg ccgcttaagt acattcccgc cgtggtacgc agggaggtga caaggctggg
     1381 acttgacagg aaccgccgct taagtacatt cgggcgatcc tggtggccgc cgaggaccgc
     1441 gctgggactt gacaggaacc gccgcttaag tacattggcg tgtcgaacat cccctcatcc
     1501 tcggcagctg ggacttgaca ggaaccgccg cttaagtaca ttccccggtc ggcgagacgc
     1561 ttctgtggga ccgctgggac ttgacaggaa ccgccgctta agtacattct atctcgatgc
     1621 tatcctgctt gttggtagct gggacttgac aggaaccgcc gcttaagtac atttcggaag
     1681 gaaatttccc cggtgtcacc gtggctggga cttgacagga accgccgctt aagtacatta
     1741 tgccccctgt taatcgtttg attaacaggg ggttctcgtc aataaaatcc atcagaaaag
     1801 catcaattga gccggcggtt gttccggtcg atgccgtttt gtccccgtaa acacctccat
     1861 cttgccgtat tggcgatccg ttatcgccag caccctcacc tccccttccg ggggcagcgc
     1921 ctctcggatt cgcttgcggc aaatgttggc ccgttcttcg ctcccgcagt acctcccata
     1981 cacggacagt tgcagcatca ggaacccttc cttcagtaaa tgcatccgga actgcgcgta
     2041 ccggcgccgg gccacgtttg aatcgaccgg cagatcgaac atcgccagca cccacatacc
     2101 gcgatactcc gacggtaccc tgtcgcccgc atgcttcgct acaccacggg caggcacagt
     2161 cctctccgtc ttcccagata cacctgtgcc aggctcgcgg ccgcatgggc gagaatatcc
     2221 ggcagcactg ccgtttcatt ttccacggcg tgccgtgtcc cgatgatccc cccgatgagc
     2281 tggcgcttcg ccgcagcgtc gagggtccct tccgggcccc gcgtgtcgca catgcccagt
     2341 acgacccggt ccaccgccgg gcgatacggt tccatcaggt cgtcggccaa gcagtaggcg
     2401 tcatagcgat tgtggtggtg aagtcccagc gacgggtgca ggcccgctcc gcagattgcg
     2461 cgcgcggcca tggcccgcag taccgcgtac ccgtagttca gcaaccggtt ctggtccgcc
     2521 cggttcctgt cccggtgaaa cgacgtccca aacaaagccg gccagtaccg ccgcgctgcc
     2581 tgcccttcga tgtttgatgt gtccccggag cgcacgtctc gcgcaagtcg aaacagcccg
     2641 aaatcctctc cccgcacccg ccgtagacaa gccccttgag cctgtacctt cgacctgacg
     2701 atctgctgcc ataatcgctt cttcagcgtt ggcggtgctt cggcctgtgc cgtaagccgc
     2761 tccccctgcg tggtatgccc cagcagcgag acgagcattc ctatcggcag gtgcttctcg
     2821 tcgcacacca cgagcgcacc tcccgcctca cagatccccg ccagcacccc ctgcgtatag
     2881 gataccgcgg gattggagac aaccagcgcc gatacatcat tgagcggggt cgtctgctcc
     2941 gaaccgtcta cgccaatgac gagttgtcga tgtcgaatgc tgaggcgggc cggttgctcc
     3001 gaaatatcaa gaaatctctc gcccatgctc ttgtcatgtt atcgacagag gcacaagccc
     3061 ccttgacctc tgccggcccc gtcagtcggc cgctcgccgc acctccccca gaggcgtcac
     3121 cacgactttg cggcagttgc gcctcctcaa gctgtccggg acagctgtca ttccggactt
     3181 tggaatctcc ttcagcctcc gagcatcgtg gatcggtacg aaccgaatct gcttggattg
     3241 agggacagtg cggaccacgt agagcgctct gcccgttccc tcgcgatcca gttcgatcac
     3301 gtccccgcag gacaacgaga agagaaaccg acccccgttg cagtcatccc gcctcacgac
     3361 cggctcccca cgccgcaacc gctgcgtcgc ctcgaaaaga ctcaccacct ttccctccca
     3421 tcgcacagcc ccgccccctt ccgcacacac ttcaaacaga gtgatgtggt gattcgcatc
     3481 cgaggtcaca tgccgccggc gatgggcgga cccgacgggg aatgtcacca ggtttttggc
     3541 caggcgcact cgccggatgg gaatccgtat tcctcctccc cctgagagat acggcttctc
     3601 cacgtccgcc gcgaacgcct tcttgacgtc tccgccggct tcctccaccc gccgccgaac
     3661 cgcggctctg acgaccgggt ccactatgtc atcaatatcg cctttgtgca gcgcggccag
     3721 cggcttgcgg atgtgcacca cggcccgccc atccacagtg cgggctccgt agaacgtatc
     3781 ctggtgcaac gccccgcgca cgcgcctcga cagccgacgg gagggtatga cccggtcgat
     3841 gaccgccttg atgtcgccga tgaatcccgg ccacggttcc tcgacatcct tgaacagctt
     3901 gtagtacgct tcagcccgcc gcgccgccgc cgtgaggcgc atgaccgtac gggtatcgga
     3961 cagcgcgacc gccaccgcgt ccacggcgtg gtgccggtgg tcatcgcgcg acttcaaatc
     4021 gtcgcgcgac agcaggccgt tcagcccatg cgcgtgccgc accagcgcgg tgatcattcc
     4081 tgccgacgcc tgaatgcgcc gctttccttc ggcgtccacc ccctgcgttc tcccgccgcc
     4141 gtagagcata ctcaaatatc tcatggccag cttggacgcg taacgtgtgt cgttgagttg
     4201 ccggctgcag aattcattga tgaatgcgtt gtgttccgcc tctctcattt caaaccggcg
     4261 cactttcgcc cagcgccccg gccccttgaa ttcccgcacc cgccccatga tctcctccca
     4321 cctctctgtc cggccgtacg cctcccacgg cgttcggcta tgcttcacct tgcgattttc
     4381 gtccgcatgg catagcgtct tgttcatgaa ggaatcatca aggcaccggc tgaaaggaat
     4441 gatgtgttcg atatcgaact gtggtgaccg cccgagcaga tcgtgtatgc caaagggctc
     4501 tgccgtgtac ggacacctcc agctgcattc ttcggcgagg aggatcttca ggatgtcgtc
     4561 ccggctcggg ttctgtatcc ccgtttcctt cctgatccgc tcggccgccc gcattctggc
     4621 ctgctcgttt tggcgcatct tcttggacaa ttccagccgc accttggcgg tctgtcgcag
     4681 gtcgcgggcc aactcgactc tgacaaaatc gggcttgccg tatcgctgca cgacggcatt
     4741 gacgacccgc ctcagttctg tcagcgaccg gatcacgagc ggattgcgaa tatcgccaag
     4801 cgcggacagc acgctcggaa gttcatctac cgcctccgtc agacgattca tccgctcgcg
     4861 gtactccggc accgcctcga ttgcgtcgta tagcttcgcc ccttgtcgga gatgcggaag
     4921 gagtttgtcc agcgcttgtc tggagaaggc gcaatggccg ggctcgagtc gaatctcggt
     4981 cagccgtttc gcttgctccg gcgcaagtcc ccacttcctc tgccctcgcc gcgccagcgc
     5041 cccgtcgttc acgatcgacc gcacgtcctc gaccaccgcc tctctgtctg cctccgtcaa
     5101 atccctccat cgctccaggc cgaacacacc cgccaactgg gccgccgttc tgtttccctt
     5161 gattctcttc tcgccgccgc gctccaggtt gaacaccgtc ccgcgcagcg agggaatgcc
     5221 ccctcttcca cgaaaactgc tgtatgtcgc ttccgccgtc gcctccagcc tttcgatgat
     5281 cgcctcccgc tcctccgacg aaagcgccct cacctccccg gttgatttgt ctatcaccgc
     5341 cagattattc acagtctgaa gataccggaa gcgctgcgcc gagaggtgct cccaagaggc
     5401 ccgtcggtac ttcggctcga gttcgcattt ccctacaaag cgtttctggc ttttcagcgg
     5461 ccgctggtag aagataatct cgcggatctt ctcgcgcaga tccgggagga gcacgtctgg
     5521 agcgaaaccg gcctgagtgt tccacagcgc gtcgaactcc tgttcataca tgtggcgccc
     5581 ggtgtaccgg tcccgaatgc gtgtgccgcc tccctcgatt gaggcgaaat actcgccgat
     5641 agtccgcgct cccaccgaag ccatctcgcg ctcgaggtcc aggattccct gtctgacttt
     5701 gcccgcctca tcctcgtcct tgggcgccga acgccggttg ctgagaaagc cgcgtcgttg
     5761 cgccaggtga taaagcgccc gtccgaattc gtagggctcc agccgctcgg aaatcccccg
     5821 acgccgcagc tgatacggcg attccatgga acaatccaac cgcttaaaga actcgtgtcg
     5881 cgccgcgctg tcccgaatgt ccgccagcgg taacagacca actgtctgta ggaagttcgc
     5941 cagccgactc gtgcgtcgtg cctgccgctc gagccgccgg cgtatcgacc gcgcctccct
     6001 gcgctgcatg tttcgcgact ttccacgggc gtctgtttcc agctcgtcca acccagcctc
     6061 gaagactcgc acgcccgccg cgactactcc cgtcggcatg ccgccctcgt gactcagaac
     6121 cgcccaccca acagaattcg tgccgatatc caaaccgagt atggtgcgac tttctttgct
     6181 cacagccaat cctcccagtc attttgcgct tgacaaagcc gctttccagc cccaaaatac
     6241 gaatgaatgt acttaagcgg cggttgtctg tcaacttcca gtaagagaca actgtctcga
     6301 aggatatgcc ctacggggta tcgacgccgg tccagaccgg cgtcactttt ttccccctac
     6361 cgcttcccca gcatcgccgc cacccgcgcc accagccgcc gcgccacctc ccctttcgcc
     6421 aagagcggcc actcctccgc ctccccatcc cgcccgatca cctgcacccg attggtgtcg
     6481 gtcaggaagc ccgagtccgc cgacggctcg ttcaggacaa tcagatcgag tttcttctcc
     6541 cgaagtttcc gccgcgcgtt ggccaccccg tcggtcgtct ccagcgcaaa cccgaccaca
     6601 atctgcccgg cccgcttgcc cgccgcgaca tccttgagga tatcccccgt cgcttcaagc
     6661 tcaagccgca agttcttgat acgcttgatc ttctcggatg agatccggga cggccgatag
     6721 tcggcgggag ccgccgccat caccaggcag tcgcactgcg cgaaccgctc cccgaccgcc
     6781 cgatgcagct cggcggtcga ctccacgcgc acgaggacca cgccctcggg ggcggccagt
     6841 tccaccggcc cggagatgag cgtgacagag gcgccctgct cggctgcggc cgcggcgatc
     6901 gcatacccca ttttcccgga agaataattt gtgatatagc gcaccgggtc gatgacctcg
     6961 cgggtcggcc cggccgtgac caggaaccta cgcccggtca ggcttttttt tttgcccggc
     7021 ccttgggcgc cgcgaagaac ccctcgatcg cccggagaat cacctccggc tcggccatcc
     7081 gccccacgcc gtagtcctcg cacgccatct ccccctcggc cggctccacc acccggtagc
     7141 cgagactgag aagggtcttg agattgcgct gcgtcaccgg gttccgccac atctgcggat
     7201 tcatggccgg cgcgatcata atcggtttgc ccgaggcgca caccaccgtg gtgagcagat
     7261 catccgaaat tcccgaggct gccttgccga taaagttggc cgttgtcggc gccaccacga
     7321 tcaggtcggg ccagtgcgcg agatcgatgt gccgtgtcgc cacatactcc ccgtgggcga
     7381 acatttcgac cgccaccggc cgcgccgaca ccgtctccat cgtcagcggc gtgatgaatt
     7441 cggtggcggc ccgggtcatc accacctgca cctcggcccc ggctttgacc agcgcacgca
     7501 cgagataggg gactttgtag caggcgatgc cgccggtgag accgacgaga atcttcttgt
     7561 tcgtcagtga catggattag ttgacgtgaa gcgaccggtc gggcgacgcc tcgcccagcg
     7621 aaagctcatc gaaatcctca aggtcggcca gcaccaccga cgacgcgatc agcttgatct
     7681 gctccagcga caccgacttc gacccgaaca ccgacacccg ctccatgatc ccctcgaact
     7741 gctcatgatc caccagcgac agattcacca gcttcagcag gaatccctgc gcctccggct
     7801 ccaactgcat ccgctccatc ggcgtgagga tccgggtcga ggcgttcatg agcgggatct
     7861 ccgagaaata ctgctccggg gtgttgtcaa accgctgcag gagccaggag taggccgacg
     7921 agatctcgtc ctgcgaatac ccctcggcct cgagcaccgt cgagagatcc tcgctcccgg
     7981 ccagattccc cttgttcccc tgcatgaaat ctataaggaa aacgactatt tcaagtatcc
     8041 gatttcgcat gtaagcgcct tgacctctac ctttcctacc tcgggcaaga tactaccgga
     8101 agttgatatt tccaaacgat atttcctctg ccggttccga gttcgtggaa agcctgtatc
     8161 atggtgaaaa tcaacttctt cggccggccc ggcgggcggc gggccgcctc cccggcccgc
     8221 aaaaaaaagg cgccgccccg aatgaggcgg cgccgcaatc agcggcatcc ggctgtgcgc
     8281 acccctatgg gcacggcacc gggatgcaca cgtcccagca cggatagggc gggatctcgg
     8341 agcagtacat cacccagatg cagcacttcg gccgcgcctg ggcattgtcc gcaaaggtcg
     8401 ccacgagtcc gccggtcaca atcgcgacga gcgcgaatat caggaccagc gtcttcatcc
     8461 tgcggagtgc gttcattggt ttcacctcct caaacggtta gggtatcgca ttctccggcg
     8521 tctccggagt tttctgctca agcttccctg cctcggccgc ccgggcctca atctccgcct
     8581 gctgcgtcga tcccgcgacc cccggccacc gccccgccac ctgcccggtt ggcgaaatga
     8641 gcagcgtctg cggcaccacc gtgccgccat aggcttccac gatgtagggg tcgccggccg
     8701 caatcaccgg gaacgcgact ccctgcgccg cggcgtaccc ccgcacttca tccggctctc
     8761 ccgtgcacat cccgatcacg cgcacgttcc gcccggcgaa ccggtgcgcc agctcgttcc
     8821 agaaggggat attgtccttt ccgcgcgacg accgcatcgt gagccagcac agcagcgtgg
     8881 ccggctctcc cggcccgtac gagagccggc agatgccgcc ggtgacatcg gccgcctgaa
     8941 gcggcggcac ctccgagccg gtctgctgcg cggccacgaa cgccgccgga tcccgaagca
     9001 tctgccgcag ccggccgttg tcgaccagga gaaagccgat cgcgatgagc gccgccgcga
     9061 gcagtatggt gaccgtcgcc gcgtaccgat agtggccgaa ttgcataatc acgtgccggg
     9121 ccggaccgcc cggtggccgc ccggtccgca ccgaagacga tgttaatgac ttgatcgaat
     9181 gcgaaagctt ggggccaata tgtcccggcc gccgaaacag tgtcaagagg aagtttttgc
     9241 ccgccccgct cccgaaaaac gcgggccggg ttccgcggaa cccggccccc atccgactcg
     9301 tagtttggca gacgcggtgc gccggtcgcc gccggcgccg gactcaatgc ttgaaatgcc
     9361 ggtgtccggt cagcaccatc gccgccccgg ctttgtcggc cgcggcgatc gcctcgtcgt
     9421 ccccctttga accgcccggc tgaatgatcg cggtcacccc cgcgtcggtg cccacctcga
     9481 cgccatccgg catcgggaaa aacgcatccg aggccatcac cgagccccgg gcccgctcgc
     9541 ccgcccgctt caccgccatg aaaccggaat ccacccgcga ggtctgcccc atcccgatcc
     9601 ccaccgtcgc cgttcctttt gccagcacga tggcgtttga cttggtgtgt ttcacgactt
     9661 tccacgcgaa cagcagcgac cggatctcct ccggcgtggg cgcgcgtttg gtcacgaccg
     9721 tgagcgcgtc cggattggtc tcgatatcgt cggtgtcctg ccgcagcagc ccgccccgga
     9781 tcggcttgtc ggccagttgc ccgcggggcc agccgagggt gatcgacggc agcgcgagaa
     9841 tccgccgcgc cttcttcttc gtcaagagcg cgagcgcctc gtcggtgaac gacggggcga
     9901 tcatgcactc gacaaacggc gtctcgtgga ggagccgggc gcacgcgagg tcaacctccc
     9961 ggttgagacc gatgatcgac ccgaacgccg acagcgggtc gctcgcgtag gccgcctcgt
    10021 aggcctcggc gatcgttgcc ccggtcgccg ccccgcacgg attggcgtgt ttcaacacgc
    10081 aggcgaacgg ttccgtgaaa tccagcagca tgtccaggca ggagtcgagg tcgccgtagt
    10141 tgttgtacga gagctccttt ccggccagga tccgggcctc cagcagcgtc ggccccccgt
    10201 acccgtcgcg cgcgtagagg gccgcctcct ggtgcgggtt ctcgccgtac cgcagcgccg
    10261 agcggagact gtaactcggg cggtactgcg gcgcgaactt cgcccccgcc tgcccggcgc
    10321 cggcgaaata tccggcgatc gccgtgtcgt agcgcgccgt cagcgcgaac gccttggccg
    10381 cgcaccggcg gcgaaacgcc cgcgtcgtcc ccccgtcgtg cgcgttcatc tccgcaatca
    10441 gctcggcgta atcgccggga tcgaccacca ccgccacccc gtcgtggttc ttcgccgccg
    10501 atcggaccat ggacggtccc ccgatatcga tattctcgat cacctcgtcg tcagtggccc
    10561 ccgccttcgc caccgtctgc tcgaacggat acagattgac gaccacgaga tcgatcagtt
    10621 tgtactcgtg ctgggtcatc gtctccaggt gcgcttccac atcccgccgc gcgagcagcc
    10681 cggcgtggat gcgcgggtgg agggtcttca cccgcccgtc cagaatctcc ggcgacccgg
    10741 tgaaggccga caccgagacc gcgtgggccc cggccttctt gagcgcctgc agcgtccccc
    10801 ccgtcgacag gatctccacc ccccgcttct ccaactcgag cgccagctgg gtgatgccgg
    10861 tcttgtccga taccgagatg agcgcgcgtt tgatcttcac tgtatcttcc atgtctctgc
    10921 ttcccctgat cgggttgtgt cattcctgct tcatgagccg cgcggctatc ggccggtgag
    10981 gaggcgctgc gcctcgcggt agcgcgccgc cgaggcctgc actacctcct ccggcagccg
    11041 cggcgccggc ggtttcttgt tccagtccag cgcgtccagg tagtcgcgga tcggctgctt
    11101 gtcgaatgag ggctgcccgc ggccgattgc gtaggtgtcc accggccaga accggctgga
    11161 atcgggcgac agcacctcgt cgatcagcat gaaccgcccg tccttgtagc caaattcgaa
    11221 cttggtgtcg gcgataataa tccccctggt ccgggcgtag tccgcggctt tcgtgtagat
    11281 cgccagcgag ctgtcgcggc accgctgcgc cgtctccgcg cccacgagcc ccaccagctc
    11341 gcggtagctg atgttgatgt cgtgcccgtc gtcgttcttg gtcgagggcg tgaagatcgg
    11401 gcgggggagc tgttccgact cgcccagagc cggcgggaac tcaaatccgt gcaccgcgtt
    11461 ggttccccgc tttcgcgcct cctgcagttc cttccacatc gacccggaaa tgtacccccg
    11521 cacgatgcac tcggcctcca cccggtcggc tttggccacg atcatcgagc gcccctcgag
    11581 ctgctcccgg taggcgcgca gcgccgccgg gtaccggtcg acgtcggccg tgaccaggtg
    11641 gctctcgacc acatcgctga ggaagtcgaa ccagaacagc gacatctgcg tgagtacccg
    11701 ccccttgccg gggatgccgt tcggcatgac cacgtcgaac gccgaaatgc ggtcggtcgc
    11761 cacgaacaac agcgtgtcgc cgagatcgta aatgtcccgc accttccccc gccgcatcag
    11821 ggggtactcg cggatctcgg tctgcatcac cacccgatcc gtctcagccg ccatattcac
    11881 ctcgaatgag tttgtccagc acccgggcgt accagcgatg ttcctgttcc agcacgcgcg
    11941 ccgccagcgt ctccggcgtg tcccccggca ggatcggcac ccgctgttgg tggagcaccg
    12001 cgccgtggtc gtacgcctcg tcaaccaggt ggatcgtcag ccccgattcc ttctccccgg
    12061 ccgccagcac cgcctcgtgc acgaaatgtc cgtacatccc cttgcccccg tatttcggca
    12121 gcagcgccgg gtggatgttc acgacccgcc gcgggtatgc gcggaggata tttcccggaa
    12181 ggagtttcag gtagccggcg agcgcgatcc actccacgcc gtgctccttc agcacgcgca
    12241 ggagcgccgc ctgcccctcc tccggggttg cgtagtccgc ctccttgtag acgaacacgg
    12301 ggatctggac ctgccgggcc cgctccagcc cgaacgcctt gcgcaggttt gacaccacca
    12361 gcgcgatctc tcccgagagg atcccgcact tcgaggcatc gatcagcgcc tggaggttgc
    12421 tgcccccgcc ggagagaaat acgccgatcc ggggccgtct gccgtctgcc atcctgtccg
    12481 tttgtccctc gcttgccgcc ttgccttcac cggccggtcc gccaaggtgc taagtatacg
    12541 ccttcgccgg ccaaagtcaa accttctctg ccgcccgcag aaacgccccc gtatccccat
    12601 caaactgctc cggtatctcc atgttcacca tgtgcccgac gccccggtgg atgatcaccc
    12661 ggctgcccgc gatccgcttg cccaactcct gtgcgagcgg cgcgaacacc ttatccttct
    12721 ccccggcgac gatcaacgtc ggcgccgtga tcgtgtgcac ccgctcgacg tcgtttcccg
    12781 gactcgggta cttcccccgg cgttcgtcca gccacgccgc ccccgagtac tcgcgcatca
    12841 tctgttccag ccggtcgcgg atggaacgct gatcctcgcg gtagacgcgg agcccgtact
    12901 cgatccaccg ctcgcgcacg tacgcgagcc cccgggcggc cgccgcggcg aaatcctccc
    12961 cctgctcctg atgcgcccgg cggcggatct ctcgcccctg ttcctccgcc cgctccatct
    13021 tccggccgat attccacccg gcggcgctcg tcgacacgag cgtcagcgac accaaccgcg
    13081 cctggtcccg cagcgcgtac ccgagagccg tcgatccccc catcgacaat cccaccaggt
    13141 gaaaccgctc aagccccagg cggtcggcga accgggcgag gtcctccacc cggtcggccc
    13201 ggctgtaccc ggtcgccggc gcatccgacc ggccgtgccc ccgcgcatcc ggcacaatga
    13261 ccggaaactc ccgcgcgagg aactcgcggg tctcgctcca catccggcgg tcgagggtga
    13321 atccgtgcag cagcaccact ggcgcccgcc cggcggcgct cgccgccggt ccgctcgtct
    13381 cgtagtagag gcgaaaatcg cccagcgaca cgtacggcat cctttgctcc tttcgcaccg
    13441 gatcatccgg caacttcccc ctggcatgat acgtcgccgc ccggcccgcg gtcaatggcc
    13501 gccgcgccgc ctgaacggcc ccgcccccaa aaagaagcgg gtccgccgcc gcggcgtgca
    13561 gccgcggagg cggacccgtt cactcgtccc ggccgtcggt ccggtctcac atgaccggga
    13621 ggtacttctc cacctcgaac gcgctcacgt ggatgcggta ctggtcccac tcaatgcgct
    13681 tgttctcgat cagcttggtg aagacgtgct cgccgagcac ttcccgcatg agctgcgact
    13741 cctcgaacat ccgcacggca ttctccagcg aattggggag gacttcgatg ttcaggcggg
    13801 ccttctgctc ttccgacatg tcgtagatat tttcctccac cgggtcgggg agcgtcaggc
    13861 gctgctcgat cccctggagc ccggccgcca gcatcgccgc gaaggcgagg tagggattgg
    13921 cccccgggtc gggggagcgc agctcgatgc gcgtggcccg ctccttgccc acacggtgcg
    13981 ccggcacccg caccatgctc gaccggttcc ggcgtcccca cgatatgtac acgggcgctt
    14041 cgtaccccgg caccagccgc ttgtaggaat tcacccactg gttggtcacc agcgtgaact
    14101 ccctcacgta tttgagaatc cccgccgtat actgccggcc gatctccgac agcccgtact
    14161 ggtcggtgcc gttggcgaac aggttcttgt cccccatgaa gagggactgg tggcagtgca
    14221 tcccggaacc gttctcgccg aaaaccggct tgggcatgaa ggtcgcgtac agcccgtgct
    14281 gcatcgccac ctgcttcacc acgaacttgt agacctgggc gaaatcggcc atcaccagcg
    14341 cctcctggta cttcaggtcg atttcatgct gcgagggcgc cacctcgtgg tgggagcact
    14401 ccaccggaat ctccatcgcc tccagcgcat tcaccgtcgc cttgcgcacc tgcgatccct
    14461 tgtcgaccgt ccccatgtcg aaatacccgt ccacgtcgat caggtcggtc gtccgctcgt
    14521 cgcgcaggta gaagtattcg agctccggcc ccacgtagaa cgtccagccg cgctcggcca
    14581 ctttctgcag ctgccggcgc aggacccacc gcggatcgcc gtcgtagggc gttccgtcgg
    14641 gcaggacgat gtcgcagaac atcatggcca gccgctcccc cgacgactcc cagggcatga
    14701 tccggaaagt caccgggtcc ggcatcgcca gcaggtccga ctcctcgatc cgggcgaatc
    14761 cttccaccga ggatccgtcg aacggctggc cttcctccag gacctgttcg atctccgttc
    14821 gcgggattga catacccttg atgcggccga gaatatcggt gaaacagagg cggatatagc
    14881 gcaccccgcc gtcgtccatt aatctgagaa tctcctgctt cgtcttcgtc atcgcttgtc
    14941 tcctggtacc ggtttgacgt tggtcgggtg acccgcccat tttggaagcg gacaataaac
    15001 taggaatggc ggtaaaaaca ataaaaatcc tgcgcggttc ggcgaaattt ccccgcaccg
    15061 tggacaaaag ggagtcggtc gacccatgat cccgccccgg tcgggggctg cccccggtct
    15121 gcgcccggcc ccggccctgc tttttccgtt gacttgcccc ccgctttggc gattattggg
    15181 aggcgggcgc cgccggcggc gccccgggat ccgggtaaca ctatgaccga caaaatcatt
    15241 tcccatccgc gcgacttccc cgccgtcgag gagctcctcc agcacccccg cctggccgat
    15301 ctcatcgccc gtttgccccg tccggtcgcc gccgacgttg tccgggccgt cgtcgccgag
    15361 gccaaatccc ggctgacgag ccaagaccgg ccgctgaccc gcgacgacct tctcgccgcc
    15421 ctccgggccg ctctccgtgc ggccggccga caggccgccg cccgggtcat caacgccacc
    15481 ggcatcgtcg tccacaccaa tctcggacgc gccccccttc cggccgatct ctttgacgcc
    15541 atcaagcact ccgtcaccgg ctacgggaac accgaattca gcctggaaac cggccgccgg
    15601 ggcggccggg gcggggcggc cgaaagctac ctcgccttgc tcgccgaggc cgaggccgcc
    15661 accgttgtca acaactgcgc cgcggcgctc tttctggccc tcaattccct ggccgcccgc
    15721 aaggaggtcc tcatctcgcg gggcgagctg gtccagatcg gcggcggctt ccgcatcccc
    15781 gatattctcc gcaaatcagg cgcccggctc cgggaagtcg gcaccacgaa cattaccacg
    15841 atcgcggact acgcctctaa tatcgacccg aagcgcaccg ggcttatcct gaaagtccac
    15901 aaaagcaatt tcacccaggc tggcttcacc gaggaggttt ccctccgcga tctggtcgcg
    15961 ctcggccgcg agcacaacct cccggtgatg aacgatctcg gcagcggcct cttcatcgac
    16021 accctccccc tccttggcta ctccgaaccg actgtccagc aatcggttcg tgccggcgcc
    16081 gtgctcacaa ccttctccgg cgacaaactc ctcggcggcg tccaggccgg cctcctggtc
    16141 ggccgggccg agatcatccg cacgatcaag aagaacccgc tctttcgcac catgcgcgtc
    16201 gacaaaatcg tcttctccgc cctcgagcgc ctcttctcca tctacctcgg tggcacctgg
    16261 caaaccgaca tcacgctctg gcgcctgctc gccctccccg aatccgagct gtacaagcgg
    16321 ggtaagaaaa tcctggccga cctggggcag cccgaggacc tctcggtgga agccacccag
    16381 gcattcgtcg gcggaggcgc cctccccgag gccgccatcc cctcggtcgc cgtgtgcttc
    16441 tccaaaaccc gcaacgccgc cgccctgctg gccgccttcc gcgaccgcga cctccccatc
    16501 atcggtcgga tcgaaaacga tcgcttcctc ctcgacctga aagcggtcga aacagaggat
    16561 ttgccggctc tcaccgaagc catccggtcg gtcctcgggt aatcggctat gatcgtcgtt
    16621 ggcaccgcgg gacacataga ccacggcaag tcggccctcg tcaaacgcct caccggcacc
    16681 gaccccgacc gtctcccgga ggagaaggcg cgcggcatga ccatcgacct cggcttcgcc
    16741 ttctaccgca cgcccgccgg cgccaccgtc gccttcatcg atgttcccgg ccacgagcgg
    16801 tttgtcaaga acatgatcgc aggggccggc gggatcgacg ccgtgatgct cgtgatcgcc
    16861 gccgatgacg gctggatgcc gcagaccgag gagcactttc aggtggttcg cctgctcggg
    16921 gtacggcacg gcctggtggt gatcaacaag accgacctcg tcgagcccga ctggctggag
    16981 ctcctgaccg cggacatcgg cgcgcgcctg gccggcacct tcctccacga cgtcccggtc
    17041 tttgcggtct ccgcggccac cggcgccggt ttcgatcccc tgcgcgaata cctcaaccgc
    17101 ctccccggcc tcgtcgcctc gcgcaaagac atcgggaaag cccgcctctt catcgaccgc
    17161 tccttcgtcc ggcccggcat cggcggcgtg gtgaccggca ccctgcgcgg cggctctctg
    17221 cgcctcggcc agacggtcgg cgtctggccc gcccgcaccg tcggcaaaat ccggtcgctc
    17281 cactccggcg gcgacagcgt cgacctcgcc gaaccgggcc agcgcaccgc cgtctccttc
    17341 accggcgtcg accgcgaaca gctcctccgc ggcggggtca tcaccgaccg caccgacctc
    17401 gagtacttcc cgcaccatcc ggtgctcgcc ctctcgctcg aacttctccc caacgccccg
    17461 gtcgccctgg aggaccggcg ccgcgtcctc ctcatagtcg gcaccacgga ggtcgagggg
    17521 gagattcgcc tcttccgcgc cgaggagatc cggcccggcg gctccggcct ggttttcttc
    17581 cagcccgacc agcccgtcta cgccctcatc ggcgaccgct gcatcatgcg cctgccgaca
    17641 ccgatggtca cgctcggcgg cggccaggtg ctcgaccacc tccgcttctt cccccgccgc
    17701 cgcgccctcg accgctacga ctatcttctc ctccggagca gcggccgact ggaggatctc
    17761 gtccgttcgg aactgcagaa acgcaccgct gtccaccgcc cgcacctgct cgaacacgcc
    17821 gactgcgccg agaccgccat caaccaggcc gtcacccgcc tcatcgactc cggccaggcc
    17881 gccctctacc ggggatacgt tttcgagccg accgcatttg cccaggctct cgagcgcttc
    17941 aagcgacgtc tcgagaaaca cttcgagcgc aagtcccacc tcaagggagt cccgatcgag
    18001 acgctccagc agctctcccc ctacggccgc gagacgaccg acctcctcat ccagtacgcc
    18061 ctggagaacc ggatcctggt gcggcacgac gacgagtact ccgtcgccgg gcgcggcatg
    18121 actctcgccg gccccgtccg caccgcctac gacgagatca tggccgcact ccgggccgac
    18181 ccctgggcgc cgcctaaact cgccgatctg acctcccgcg gcaaggcgca ccgcgaggcg
    18241 atccgcttca tcctcgactc ccgcgagggg cacaagtgcg gcgccgattt catcttcctc
    18301 gccgacacct ggcagaccgt cctcgcctac atccgcgaac ggctcgaccg gaccggcgaa
    18361 ctcgccatca ccgacctccg cgaccgcttc ggcttcaccc gcaaattcgc cgtgcccatc
    18421 ctcgaggaaa ccgatcgcct ccggctcacc cgccgcgagg gcgacctccg cgtcaaagga
    18481 gaacgctatg aagtcccgct cgctgatcta taacgccaac atcctgactc aggaccgccg
    18541 cctcgcggtc gattcgatgg cgatcgagcg tgggcggatc gtcgccgtcg gcgacaacct
    18601 ccgcggcgtc ggccctttcg ccggtttcga ccgcatcgac ctcaggcgcc ggaccgtcgt
    18661 gcccggcttt gtcgacgccc acacccactt ctactactac gccctctccc tcgggcgcat
    18721 cgacctccac ggcgtcgatt ccctggacga gtgccttcgc ctcatccgtg gcttctgccg
    18781 cggccttggc cccgatgact gggtggtcgg tgagggctac cgccccgatc tcttcgccgg
    18841 acgagtcgaa ccggaccgcc gccagctcga ccgagtggtc ggccgccgcc cggcgttcat
    18901 tttctcgcat gaccagcact cggcctgggt gagcacgcgg gcgctggaac tcgccggcct
    18961 cacccggcgc acgccggatc cggccggcgg agtcatcgag cgcgaccccg atggttctcc
    19021 gcgcggcctc ctccgcgaga tcccgggcta cgacccggtg ttccggctca tacccgcgcc
    19081 ctcgcggccg ctcctcgacc gcctctatgg gcgggccctg gcgcaggcct accgccgggg
    19141 cgtcaccggc gtgcactcgt tcgatggtcc ggcggggttc gcctggttca tcgaacgcgc
    19201 cgcccgcggt gcgctcggcc tccgcatcaa ctactacgcc cccgccccgg gcctgcccga
    19261 actgcgcgct gccggcaccc gctacggcgc cggcgatgac ttcctccgcg tcgccggcat
    19321 caagattttc gccgatggtt ccctggggag ccggaccgcc ctgtgctatc agccgtaccg
    19381 cgacgaccgg cggaaccgcg gcgtcgaggt caccccgccg ggcaagatcg cttcgcttgc
    19441 gcgcgcggcc gcccgcctcg gccttccctg cgccatccac gccatcggcg accgcgccgt
    19501 cgcgaatgtc ctcgacgcgc tcgcggccgc cccgcccctc cgcccgcccg cccgccaccg
    19561 catcgaacat ctccaactgg tccgccgccg ggatctcccg cgcctgaaga aattgaatat
    19621 tgtcgcctcc gtgcagccgc agcagacggt ttccgacatc gagatggtcc gcgccgcctg
    19681 gggcgggcgc gccaaggacg cttacatctt ccgcgccctg ctcgatctcg gcattgatct
    19741 ggcgttcggc tcggacgtgc cgattgagcc gctcgatccg ctcgccggaa tcgccgccgc
    19801 cgtccgccgc gcccacggac ccggcgaggc cttccccgat tccggtcccg ccggaaagcg
    19861 ggcccggcgc g
//
LOCUS       DAOPOE010000060        19094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3507_length_19094_cov_4.789065, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000060 DAOPOE010000000
VERSION     DAOPOE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3507_length_19094_cov_4.789065"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKM94_08955"
     CDS             <1..238
                     /locus_tag="PKM94_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66805.1"
                     /translation="QVIDEAVQELPEKYRLAFMLRDLQEMPYEEVARILSIPLGTVKS
                     RVNRARLMLRDKLLPKLEGQNALSKNTLLPVSLL"
     gene            270..878
                     /locus_tag="PKM94_08960"
     CDS             270..878
                     /locus_tag="PKM94_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66806.1"
                     /translation="MSEHLSTCPECRREESEYRSLFAAAEQVKARTVSADFTTKLFHR
                     LAEERFAETRTKAYLPKRAPVVRWAAVAPVAVSVCVLTLVGIMMLSGGQEPQTAMADL
                     GGDPSTRYMTAQPTHNPNLTVNLEKDWSLAAQMARAERLNRLSQAVGAQVEFHGLPTY
                     GATNVAATTGASPYVSEVYRIRPVIRSYQTPDGTAREVDQTY"
     gene            1002..2060
                     /locus_tag="PKM94_08965"
     CDS             1002..2060
                     /locus_tag="PKM94_08965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOD66807.1"
                     /translation="MNELIYRASRSIVTVEAIQPRMYGGVGGAGGQTIHTLVSSGLIY
                     DSAGHVLASASSVAGREQILVHYDDQTLEARLCGVDYITGLAVLAVDRPLGVPAALCR
                     HRQCAGQMVIAIGNAYGVRASPTLGFCSGLRNDGIMQFTAPITSGTVGGGVFDMAGEL
                     VGVITGGIGRERFAEVGLAVPASEIPEAVAHLVAQGDRYAGYVGVQIADIDVSPGLEI
                     RYPASLVESGRTPGRIIDRGVLVTSVTAGSPAARAGLSPGDLLFAVNGVAVGSAGELR
                     SLVRNNPPGTRVEIGYLRREMPYVVALETARLDDPRWEDLFAPQPGVSDPAEVTDSLR
                     KEVERLQRALEDLQKRLR"
     gene            complement(2128..2859)
                     /locus_tag="PKM94_08970"
     CDS             complement(2128..2859)
                     /locus_tag="PKM94_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOD66808.1"
                     /translation="MGLIADALVWKGNRLEARVLYSRAGVAQHILVRTDRSGILVDTG
                     DGCLRDLLAAGIRLDSLGGLLYTHGHFDHVGGLHTLLGFLRMIGRTAPLPICAPDRCT
                     EVFALAHTFRRCYPDHLPYPLVLRELHPHERFELAGLPIEAYPMAHAGSIDRGGILDP
                     IPAFGYRITLEGETIAITGDTGLCDEVKDLVRGADLAIIEATFADDDRCEPEMLARVH
                     LSESLARDLGTLAKSYLLIHRAERS"
     gene            3094..3999
                     /locus_tag="PKM94_08975"
     CDS             3094..3999
                     /locus_tag="PKM94_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD66809.1"
                     /translation="MIRLLLVLTVCVWGWSFVATKICLAYLTPLQVIGLRYLLGLPVM
                     GAVLLAKRRPIRVPRAEYPWILAAAAAITIHFLVQVIGMQYTTATNTGWLIAVTPLVL
                     AVLSAVFLKERLSAKAAAGIAVATTGVLLLVSQGRLFTLGWLQSAGDWLVFASAHTWA
                     VFTILTRDLSRRCGPLVTPFLVLSVSAAVILTLMFGSAGLAGVTALPVRGVVALLFLG
                     LVVQGLAFWFWQEGVAKLGAARAGVFLYLEPLATTALAVPYLGEHFGLFTGIGGGLVL
                     LGVYLAERRVRRGGAANRAGTLESR"
     gene            4150..7614
                     /locus_tag="PKM94_08980"
     CDS             4150..7614
                     /locus_tag="PKM94_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOD66810.1"
                     /translation="MPLAPELVEKLRASGELPLVAASLKEARAQGVWAPNPSIGSPDK
                     AAGQAFQLAPGAVDTFRVLVILVDFSDQPASGGLVYGQPADFMHLLFSFDPGDNHYSM
                     AEFYRDNSYGTFIMQGQVVGWVRAPQTYAYYVDGQKGFGTYPQNAQKMAEDAVLLADP
                     LVNYSMFDADGNGWVDGVFIVHSGTGYEQTGDPNDIHSHMWSLRTSQYLDGVTISSYT
                     TEPEENSATGLSTMGVYAHEYGHFIGLPDLYDGDYSSSGIGDWSLMAGGSWNIGGRKP
                     AFMDAWCKKELGFLTLTNITAGAENVAIPSSYHEPAAYRLWTNGAVGSQYFVIENRRR
                     TGYDVGIPGSGLLIYHIDETIGGNWDENHPKVAVEQADGEFELESGANQGDGGDVWSA
                     GTKADFDDLSTPNTRAYGGAVTGTAVWTISASDSVMTAGFDIALTRPRFQLLSGVFSD
                     AAFGNGNGIVETGERMTFTFTLQNAWATATNVTGTLTADNNDIVFITPTVNIGAVAGS
                     GGTGGNALNPIIFDVPADFSPCIDSFFLTVDADQLGAERTYGFELHVGGPEVLLVDDD
                     NGANWNQAYEQALFGLRVPFAKWTKAVSGTPPADTLSRYAIVVWFTGDDRPDVLSAAD
                     VAAMTTFLDNGGNLFLTGQVIVRELNGDAPSFLSNYLKAGYLGDQYYPIMNGAAGSAI
                     GDGYKLRYNVGDNQDDPQKMTAINGSAAEFELPVGGVTMLSWAGSYKIVLASFGLEGV
                     ADGYVANGYATKQMVLQRVLDFFAADSVSQNPSITAVAVGGGTSPLRLVDHTPEFSWS
                     VVDTAGGPAVAYEVQVGTGDLCFNADDMWSPGAAAGADTSAVYAGAPLADGQDYVFRV
                     RVSNGASWSEWERLEFGMNAVSASGLLVAPAQDSLVTTATPTLKVTNTTDPDGDVQTY
                     EFAVYADQGLTTVVAAAAGVTQAPPHTVWTVSTPLADDARYWWRARAWDGYEYSPWSA
                     VGTFLVNAVNQAPAAFSLLAPANGDTVAVHPTFVWQASTDGDLGDAVSYTLKYSEDSL
                     FALYDYVQLAETTVVLFSPLDEGTRYFWKVEARDRFSGMTVSTETFRLTTQTTGCCTR
                     RGDYDNDGSIKVSDVTLLVGYLFRGGVPPVCAEHGDANGDGEIKVSDLTLLVGYLFRG
                     GAAPAPCP"
     gene            complement(7693..8193)
                     /locus_tag="PKM94_08985"
     CDS             complement(7693..8193)
                     /locus_tag="PKM94_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD66811.1"
                     /translation="MNISRYMTEDAVKLEMETVIDPPEEGQSIERWREQAKSLILLEL
                     VDLLNETHRVGNRNKLLIDFVNREKKASTAIGHGIAFPHIRSMQATEFMIAFARSHTG
                     YDYGAPDSAPVRLFFIMAAPPYEDNLYLKVFKALAEMLQYESFREELLSVTSPGEVLR
                     AIRAME"
     gene            8590..10413
                     /gene="aspS"
                     /locus_tag="PKM94_08990"
     CDS             8590..10413
                     /gene="aspS"
                     /locus_tag="PKM94_08990"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOD66812.1"
                     /translation="MVKFSEVKRTHSCGELRPAHVGTTVRLNGWVNSYRNLGGLFFLD
                     LRDRYGITQVVINPETFDKDMLAEAQRVRHEYVVAVTGKVRHRPEGTANERMLTGGIE
                     VVAEAFWILSESKTPPFEIEEETSASEKLRLEYRYLDLRRGPLQERIRFRHDLTMCVR
                     KYMSAQDFLEIETPLLIRSTPEGARDYVVPSRVHHGRFYALPQSPQLFKQILMIAGFD
                     KYFQIARCLRDEDLRSDRQPEHTQIDVELSFATPEDVFAVIEGLMANVFENLAGAAVA
                     TPFPRYTYAEAISRWGIDKPDLRFGMEIVDLTEIAGASEFKVFRENAARGGVIKGIVL
                     SGGAAYSRKQLDELTELAKQQGAGGLAYILRTAEGDKSPILKFLGEATAAAMSAQAKA
                     ATGDALFLVSDRPAKTEAILGQLRLHLGRAHRLAARNEWRFLWVVDFPLFEFDEESRT
                     LQAAHNIVSHPMEEDVPLIEAGFASPLPVSDPGHPWRRARAMQYDLVLNGWEIASGGQ
                     RINRRDLQQRILNILGIDDERAERMFGFLLRALEYGAPPHAGIAAGLDRLAALMTGGE
                     SIRDVIAFPKTTNAMSLMDGSPAEIDPHQLAELGLAIREEQ"
     gene            10433..11416
                     /locus_tag="PKM94_08995"
     CDS             10433..11416
                     /locus_tag="PKM94_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOD66813.1"
                     /translation="MNAHLDESVVRSFTIEGIDQRLLFGQNDVFLRIIEARFACRIVA
                     RGDKIVIDGLPDVVEKVVRLLNDLVTRIKQGDAVTEQYLNYAIVMVKESGLGPAQDIS
                     TQSLLTSSLKREIRPMTIGQTKYVEAVSANDIVFSIGPAGTGKTYLAVAMAVAELKAG
                     RVNRIVFTRPAVEAGESLGFLPGDIRAKVDPYLRPVYDALYDMMQPDKIRRLLELGII
                     EIAPLAFMRGRTLNEAFVVLDEAQNTTSAQMKMFLTRIGEKSKAIITGDITQIDLPDR
                     RGSGLVKVQGILKGIEGIAFIYLTDRDVIRHRLVQDIIRAYEAHEGRSKKR"
     gene            11431..13680
                     /locus_tag="PKM94_09000"
     CDS             11431..13680
                     /locus_tag="PKM94_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD66814.1"
                     /translation="MLITSILLRIKRAIKRLLKVQSESRLTITDTARSRATKAAMLAA
                     VALAVGILYPGEELYDPFDVPRRGEVAPADVVAPFAITVGKTEREIEDEEEMVRLAVP
                     FVVDYDSTAVRQALGGLHRFLAAADSVSRLSRWQARVDEIADTLEARYARLTRSAILD
                     ALGRGNLRDVETRLRRIYEEDIYRVGVLPSKNSIPESRNKNVLIRRGERENIHHRDKL
                     LFLEMADAQLLSTLNRLAATEPIDVEFHYLVGRAFLQPNLHVNMAEYTRRLDEELTQI
                     QTVKETVEEGAVVVRAGSRVNERQERILRQLVQMQRARAAEQSWLLNLVPAIARVILV
                     LGAFSTLFLFLFHFRREVFYSNERLIAILTVYAIQLFLIYLLDRWNLSVYLYPVAFLP
                     IVLTILFDAEVGVLSAIVLAVLVGIVHRFDFTLTFMTVVAGTVAVFSARRVRRRAHFF
                     RIMIYTAFAYAVFILFVENLKLAPSTEIPALMAYGLANGFACVILAIGILPVFESVFG
                     ITTDITLLELSDLNHPLLKRLALEAPGTYHHSIIVGNLSEAAAEAIGGNSLLARVGAY
                     YHDIGKMEIPEYFVENQLSVKSKHESLSPSMSALILSSHVKKGRALGEAADIPDDVLN
                     FIEEHHGTMVQKYFYDKALRAGNGEAVMDKFRYPGPKPQTRETGIAMLADAVEAASRT
                     LDHPSPARIDNLIQRIINDRFESGELDQCPLTLRDLAKIKRAFAQVLIAAFHHRVEYP
                     KDEKAEERV"
     gene            13677..14108
                     /gene="ybeY"
                     /locus_tag="PKM94_09005"
     CDS             13677..14108
                     /gene="ybeY"
                     /locus_tag="PKM94_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOD66815.1"
                     /translation="MKLRIYKETTVRVPRARLERLFRAVARGERRKAGEVNLVFTGGR
                     RMRRLNRTYRGRDKATDVLAFPLHEGAGEQADEIYGEVYIAVPVARAQAAAYGVGLGE
                     EILRLACHGWLHLFGYDHHTPAEAAAMRTREERYLAAVRGA"
     gene            14113..15372
                     /locus_tag="PKM94_09010"
     CDS             14113..15372
                     /locus_tag="PKM94_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD66816.1"
                     /translation="MLDLLYVFAIIAGFATAYVISLFSLAVYVDPEEVEKLSPNQSAR
                     RRGFVKKLANDPLALVQSATVYKSFALIIVTVAMYQLLPRLGAISGWPTTVVRLFGML
                     LTWLLYIFFVEYMPRRSAAAADRARLLRHLWLVGIIYFLFFPLVGRYRRALQRIRPPK
                     DVTGEDKEDLVERALETLAEQAGIGERILEEEKKEMIGQIFQLDQTVAREIMIPRIDI
                     VGIEKSMTFPEIRRLVMRDGHSRYPVYEGSIDRILGLAYVKDMFNHLPEPGEEFRVTD
                     HMRKAYFIPGTKVIGELLREFKHKRMHIAVVVDEYGGVSGLVTLEDIIEEIFGEIRDE
                     HDWEQEQIVKLTDGAFLVNAGLLVEDLQDFLDTDFEQSDYDTVGGLIYDLVGSVPREG
                     QVIRWHDLEFEVERVQGQRIVAVKVRR"
     gene            complement(15439..15858)
                     /locus_tag="PKM94_09015"
     CDS             complement(15439..15858)
                     /locus_tag="PKM94_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66817.1"
                     /translation="MNTAKTTILVVDDEPAIRQILLRSLSSAGFDTTTATDGADALLK
                     MAQQPFDIVITDITMPHMDGVALLGEIRSRFPDTKVIFITGQRLALMDKAVREADVDG
                     LISKPFRNHEIVGQLRQLCLCRTHHRAPSDPVPPAPA"
     gene            complement(15961..16179)
                     /locus_tag="PKM94_09020"
     CDS             complement(15961..16179)
                     /locus_tag="PKM94_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66818.1"
                     /translation="MALATKPVTRCPFCKETILEGAVKCKHCHSELSAPPAARPKRLA
                     RLNTFRIGFLVGVVFTLILVILVYRHFA"
     gene            complement(16242..16577)
                     /locus_tag="PKM94_09025"
     CDS             complement(16242..16577)
                     /locus_tag="PKM94_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOD66819.1"
                     /translation="MQQQPPQQQIAVEFDEKAAEGIYANLALIMHSPTEIIIDFARVM
                     PRLPKAKVLSRIIMTPMHAKLLHQALTENLKKFEAQFGEIKTFGFPGGGKGPIGFESG
                     VRFVDPEKE"
     gene            16713..17747
                     /gene="rfaE1"
                     /locus_tag="PKM94_09030"
     CDS             16713..17747
                     /gene="rfaE1"
                     /locus_tag="PKM94_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOD66820.1"
                     /translation="MSLSAERIAEVIARLGKARVLILGDIMLDEYLFGRVDRISPEAP
                     VPVVEISHDKLLLGGAANVAANIRALGDEPLLVGVVGEDEAAVKLSQLLKQHGIGADY
                     CVCDRSRQTTIKTRIMAHSQQVVRADRENRHELHPDVERMVLAKFTAAAESVNAVIIS
                     DYGKGVITLSLLNSVMEVCRKRGLFVAVDPKETHFHNYKRVSLITPNHHEAGFAAGRR
                     IVSEADLIDVGNSLLRELEARSILITRGPDGMSLFEQDAEPTHIPTFARKVFDVTGAG
                     DTVIAAFVAAVCAGATLVEAAIVANAGAGITVGEVGTATVSSAELYAELLRNVKNGTL
                     VTRGSTGTQA"
     gene            17819..18253
                     /gene="rfaE2"
                     /locus_tag="PKM94_09035"
     CDS             17819..18253
                     /gene="rfaE2"
                     /locus_tag="PKM94_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOD66821.1"
                     /translation="MAARHRRRGHKIVFTNGVFDILHRGHVEYLQKAKAMGDILIVGL
                     NSDASTRRLKGPERPVQSQADRAAVLLALRAVDYVVIFGEDTPYELIRQVRPDVLVKG
                     SDYKLGEIVGRDIVRSHGGTVRRVKLTPGRSTTQVLRKAAKG"
     gene            complement(18289..>19094)
                     /locus_tag="PKM94_09040"
     CDS             complement(18289..>19094)
                     /locus_tag="PKM94_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD66822.1"
                     /translation="ALSVGTDDEGSVYVAGELGEYETADCIFIKYSAAGVFPWDWTYS
                     GPAGLRDYIGDLHVGGDGSVWLSIVRDYDFFGYDPRSLVGVRLNSDGNERWARTFYSP
                     SPTGDGCESGGTSVAVDANQNAVFGYSPCWPHTVKYSPEGDLLWWRPFAVDSAGLGTF
                     RDLAADQDGNVYVIGEVPQQVSGTEWLEDIVLYKYPASCCLLRGDMNESGGVTVADIT
                     FLIGYVFRGGPPPYCLTEADVSGDGHVTVVDVTSLIAYVFRGGPAPAGC"
BASE COUNT         3293 a         5945 c         6579 g         3277 t
ORIGIN      
        1 ccaggtcatc gacgaagcgg tgcaggaact cccggagaag taccggctgg cgttcatgct
       61 ccgcgatctt caggagatgc cgtacgaaga agtggcaaga attctgagca taccgttggg
      121 gacggtcaag tcgcgagtga accgggcccg cctgatgctc agggataagc tcttacccaa
      181 actggagggt cagaatgcgc tgtcgaagaa cacgctcctt cctgtcagcc tactgtagag
      241 aggaactctc cggccggcgg aaactggcgg tgagcgagca tctatcgacc tgccccgaat
      301 gtcgccgaga ggagagcgag taccgtagtc tctttgccgc ggccgagcag gtgaaggcgc
      361 gcacggtgtc ggccgacttc accaccaagc tgttccatcg gctggcggaa gagcgcttcg
      421 cggaaacgcg caccaaggcc tacctgccga agcgcgcgcc ggtggtccgg tgggcggcgg
      481 tggccccggt cgccgtgagc gtctgcgtcc tgacgctggt cgggatcatg atgctctcgg
      541 gcggtcagga gccacagacg gcaatggccg acctgggtgg cgatccctcg acccggtaca
      601 tgaccgcgca gccgacgcac aatccgaatc tgacggtgaa tctggagaag gattggtcgc
      661 tggcggcgca gatggcccgg gccgagcggc tcaaccggct ttcccaggcg gttggcgcgc
      721 aggttgaatt tcacgggttg ccgacgtatg gggcgaccaa tgtcgcggct acgaccgggg
      781 cctcgcccta cgtgtcggag gtctatcgga ttcgtccggt aattcggtcg taccaaacgc
      841 cggatggcac tgcgagggag gttgaccaga cctactgacg aatgctaacg cacctgtttt
      901 tgaccgccag caagcgcgca cgggcgtgcg cactggcggt tctttttgcc atctcatcgg
      961 tcggcgccgc cgacagcccg ctcctccggc tggaggacgg cgtgaacgaa ctcatttacc
     1021 gcgcctcccg ctcgatcgtg acggtggaag ccatccagcc ccgaatgtac ggcggggtgg
     1081 gcggggccgg ggggcagact atccacaccc tcgtctcctc ggggctcatc tacgacagcg
     1141 ccggccatgt tctggcctcg gcctcctcgg tcgccggccg cgagcagatc ctggtccact
     1201 atgacgacca gacactcgag gcgaggctgt gcggcgtgga ctacatcacc ggactggccg
     1261 tactggctgt tgaccgcccc ctcggcgtac cggccgcgct gtgccggcac cgccagtgtg
     1321 cagggcagat ggtgatcgcg atcgggaatg cctacggcgt gcgggcctcg cccacgctcg
     1381 ggttctgcag cggcttacgc aacgacggaa tcatgcagtt caccgcgccg atcacctcgg
     1441 ggacggtcgg gggcggagtg ttcgacatgg ccggcgagct ggtcggcgtc atcaccggcg
     1501 ggatcggccg ggaacgcttc gcggaagtcg gcctggctgt gcccgcctcg gagattcccg
     1561 aggccgtggc ccatctcgtc gctcagggcg accgctacgc cggctatgtc ggggtgcaga
     1621 tcgcggatat cgatgtctct cccggcctgg agattcgcta cccggcgtcc ttggtggagt
     1681 cggggcggac gccggggcgg atcatcgacc ggggagtgct cgtgacctcg gtgaccgcgg
     1741 ggtcgcccgc ggcccgggcc ggcctctcgc cgggagactt gctcttcgcc gtcaacggcg
     1801 tcgccgtcgg atcggccgga gagctgcgca gccttgtgcg caacaacccg ccggggacgc
     1861 gcgtggagat cgggtacctg cgccgcgaga tgccctacgt cgtggcgctc gagacggcgc
     1921 ggctcgatga cccgcggtgg gaggatctct tcgccccgca gccgggagtg agcgaccccg
     1981 ccgaggtcac cgattccctg cggaaggagg tggagcggct ccagcgggcg ctcgaagatc
     2041 tccagaagcg gctccgctag gatcgtcaat ctcgcgcgcc gccccgctct cgctcgctcc
     2101 caccggcacc ggatacctgt gccctcctca cgatcgctcc gcccggtgaa tcagcaggta
     2161 ggacttggcc agggtgccga ggtcgcgggc cagcgactcc gacaggtgca cgcgtgcgag
     2221 catctccggt tcacaccggt cgtcgtcggc gaaggtcgct tcgatgatcg cgaggtcggc
     2281 gccgcggacc agatccttga cctcatcgca caggccggtg tcgccggtga tggcgattgt
     2341 ctctccctcc aaagtgatgc ggtagccgaa ggcggggatg ggatcgagaa tgccgccgcg
     2401 gtcgatgctc ccggcgtggg ccatcgggta cgcctcgatc ggcagccccg cgagctcgaa
     2461 ccgctcgtgc gggtggagct cgcgcagcac cagggggtag ggaaggtgat cggggtaaca
     2521 gcggcggaaa gtgtgggcga gggcgaacac ctcggtgcag cggtcggggg cgcagatggg
     2581 gagcggagcg gtgcggccga tcatgcggag aaacccgagg agcgtgtgga gcccccccac
     2641 atggtcgaag tggccgtgcg tgtagagcag accgccgagc gaatccagcc gaattccggc
     2701 ggccagaaga tcgcgcagac agccgtcgcc ggtgtcgacg aggatgccgg aacggtcggt
     2761 gcgcacgaga atgtgctggg ccacgcccgc gcgggagtac agcactcgcg cctccaggcg
     2821 gttgcctttc cagacgagtg cgtcggcgat caatcccatg gtgtcctttc cgctgacagc
     2881 gaccgaagat ttcgcgcacc cgcggcgcgg cgcaggccgg acggcggggc ggctcccttg
     2941 cccggtatgg gaagctgcgc caatatcggt cacgccgccg ggcggcgcaa gcggggaatc
     3001 ggtccccccc cgaggggcgg cgattccatt tgacaaggga gcgggcggag cggtaattgc
     3061 ccgtcacatt ccccggtgca gtgtggagaa atgatgattc gcctcctctt ggtgctcacc
     3121 gtgtgtgtct ggggatggtc gtttgtggcg accaagatct gcctggcgta cctcaccccg
     3181 ctgcaggtga tcggcctgcg ctacctgctc gggcttccgg ttatgggggc cgtgctcctg
     3241 gccaagcggc ggccgatccg ggtgccgcgc gcggagtatc cctggattct ggcggcggcg
     3301 gccgcgatca ccatccattt cctcgtgcag gtgatcggga tgcagtacac cacggcgacc
     3361 aacaccgggt ggctgatcgc ggtgaccccg ctcgtgctgg cggtcctgtc ggcggtgttt
     3421 ctgaaggagc gcctgtcggc caaggcggcg gcggggatcg ccgtggccac gaccggcgtg
     3481 ctgctgttgg tgtcgcaggg gcgcctgttt acgctcgggt ggctgcagag cgccggggac
     3541 tggctcgtat ttgcctcggc ccacacctgg gcggtgttca ccatcctcac ccgcgacctc
     3601 tcccggcggt gcggaccgct ggtgacgccg ttccttgtgc tctcggtctc ggcggcggtc
     3661 attctcaccc tcatgttcgg atcggccggc ctggccgggg tgacggcgct gccggtgcgg
     3721 ggggtcgtcg ccctcctctt cctcggattg gtcgtgcagg ggctggcgtt ctggttctgg
     3781 caggaggggg tggcaaagct gggcgcggcc cgggccgggg tgttcctcta tctggaaccg
     3841 ctggcgacca ccgccctggc ggtaccctac ctgggggagc acttcgggct gttcaccggg
     3901 atcggcgggg ggctggtgct gctgggcgtg tatctggcgg agcggcgggt gcggcgcggc
     3961 ggcgccgcca atcgtgcggg caccctcgaa tcgcgttgac aattccggcc gataaagtat
     4021 tttgaaagaa agagttgttc aggaggagat atcttgccta cctctcgtac ccgccgacgc
     4081 ttaactgcca gtctgttact cgccattgcg gctctgcttc cgcttgcggt tgtcgatgcg
     4141 ccccgcgcga tgccgctggc gccggagctt gtggagaagt tgcgggcctc gggtgagctg
     4201 ccgctggtgg cggcgagcct caaggaagcc cgcgcccagg gggtctgggc gcccaacccg
     4261 agcatcggat cgccggacaa agccgccggc caggccttcc aactcgcgcc tggggcggtc
     4321 gacaccttcc gcgtgctcgt gatcctcgtc gatttttccg accagccggc ctcgggggga
     4381 ctggtgtacg gtcagcccgc cgatttcatg cacttgttgt tctcgtttga cccgggcgac
     4441 aaccactact cgatggccga gttctatcgg gacaactcct acggcacgtt catcatgcaa
     4501 ggacaggtgg tcggctgggt ccgggcgcct cagacgtacg cctactatgt cgacggccag
     4561 aaggggttcg ggacctaccc gcagaatgcg cagaaaatgg ccgaggatgc ggtgcttttg
     4621 gccgacccgc tagtgaacta ttcgatgttt gacgctgatg gcaacggctg ggtggacggc
     4681 gtgttcatcg tccactccgg caccggatac gagcagacgg gggatcccaa cgacattcac
     4741 tcgcacatgt ggagtctgcg cacctcccag tacctggacg gcgtgaccat cagctcctac
     4801 acgaccgagc cggaggaaaa ctcggccacc ggcctgtcga caatgggcgt gtacgcgcat
     4861 gagtacggcc actttatcgg cctacccgat ctgtacgacg gcgactattc gtcctcgggc
     4921 atcggcgact ggtcgctcat ggcggggggg agctggaata tcggaggacg taaaccggcc
     4981 tttatggatg cgtggtgcaa gaaggagctc gggttcttga ccctgacgaa catcaccgcc
     5041 ggcgccgaaa atgtcgccat cccgagcagc taccacgagc cggccgccta ccggctgtgg
     5101 accaatggag ccgtcgggtc gcagtatttc gtgatcgaga accggcggcg aaccgggtac
     5161 gacgtcggca tccctggctc aggactgctc atctaccaca tcgacgagac catcggcggg
     5221 aactgggatg agaaccatcc caaagtggcg gtggagcagg ccgacggcga gtttgagctc
     5281 gagagcggcg ccaaccaggg ggacggcggc gacgtctggt cggccgggac taaggcggat
     5341 ttcgacgatc tgtcaacgcc caacacccgg gcctacggcg gcgccgtcac cggaaccgcg
     5401 gtgtggacca tctcggcctc cgactcggtc atgaccgccg gcttcgacat cgccctcacg
     5461 cgcccgcgct tccaactgct atcgggcgtg ttttcggatg cggctttcgg caacggcaac
     5521 ggaattgtcg agaccggaga gcgcatgacc ttcaccttca cgctgcagaa tgcctgggcg
     5581 acggccacca acgtgacggg gacgctgaca gcggacaaca acgatatcgt attcatcact
     5641 cccacagtca acatcggggc cgtggccggg tcgggcggca ccggcggcaa tgccctcaat
     5701 ccgatcatct tcgacgtgcc ggccgatttc tcgccgtgca tcgactcgtt cttcctgacg
     5761 gtcgatgccg accagctcgg tgcggagcgc acctacgggt tcgaactgca cgtcggcggc
     5821 ccggaagtgc tgctggtgga cgacgacaac ggggccaact ggaaccaggc gtacgaacag
     5881 gcgctcttcg gattgcgcgt gccgttcgcc aaatggacga aggcggtgtc tggaacgccg
     5941 ccggccgaca ccctatcgcg ctacgcgatt gtcgtctggt tcaccggcga cgaccgcccg
     6001 gacgtgctct cggcggcgga tgtggccgcg atgacaacct tcctcgacaa cggcggcaat
     6061 ctgttcctca ccggtcaggt cattgttcgg gagttgaacg gggacgcccc gtcgttcctg
     6121 agcaactacc tcaaggccgg ctaccttggg gatcagtact acccgatcat gaacggggcg
     6181 gcggggagcg cgatcggtga cggctacaag ctgcgataca acgtggggga caaccaggac
     6241 gacccgcaga agatgacggc gatcaacggg tcggcggccg agtttgagct gccggtgggc
     6301 ggcgtgacga tgctctcctg ggcggggtcc tacaagatcg tgctggcgag cttcggtctt
     6361 gagggcgtcg ccgacgggta tgtcgccaac ggttacgcga cgaagcagat ggtgctgcag
     6421 cgggtgctcg acttctttgc cgccgactcc gtgtcgcaga atccgtcgat cacggcggtg
     6481 gcggtgggcg gcggaacctc gccgctccgg ctggtcgacc acacgccgga gttctcgtgg
     6541 agcgtggtcg acacggccgg gggcccggcg gtcgcatacg aggtccaggt ggggacgggg
     6601 gatctctgtt tcaacgccga tgacatgtgg tcgccggggg cggccgccgg cgccgacacc
     6661 tcggctgtgt acgcgggcgc gccgctggcg gacggccagg attatgtctt ccgggtgcgc
     6721 gtgtccaatg gcgcgagctg gtccgagtgg gaacgcctgg agttcggaat gaacgccgtg
     6781 agcgcctcgg gactcctcgt ggcgccggcg caggattcgc tggtgacgac ggcgacgcct
     6841 acgctcaagg tcaccaacac caccgacccc gatggggacg tgcagacgta cgagttcgcc
     6901 gtgtacgccg accagggatt gacgacggtg gtggcggccg ccgccggggt cacgcaggcg
     6961 cccccgcaca cggtgtggac ggtgtcgacg ccgctagcgg acgacgcccg atactggtgg
     7021 cgggcgcggg cctgggacgg ctacgagtac agcccgtggt cggccgtggg gacattcctc
     7081 gtcaacgccg tgaaccaggc cccggcggcc ttctcgcttc tcgcgccggc caacggagac
     7141 acggtcgccg tgcacccgac ctttgtctgg caggcctcga ccgacggcga cctgggcgac
     7201 gccgtctcgt acacgctcaa gtattcggag gattcgctgt tcgccctgta tgattacgtg
     7261 cagctggccg agaccacagt cgtgctgttc agcccgctcg acgaaggcac gcggtatttc
     7321 tggaaagtgg aagcccgcga ccgcttctcg ggcatgacgg tgtcaaccga gacttttcgc
     7381 ctaaccaccc agacgacggg ctgctgcacc cggcgcggcg actacgataa cgacggttca
     7441 atcaaggtca gcgatgtgac gctgctggtc ggctacctct tccgcggggg cgtgccgccg
     7501 gtctgcgccg agcacgggga cgccaacggg gacggtgaga tcaaggtgtc ggacctgacg
     7561 ctcctggtcg gctacctctt ccggggcggg gcggcgccgg cgccgtgtcc gtgagccttg
     7621 cgccagtttg acttttgccg aaccgggtcc ctcggggccc ggttttctct tgccggtgat
     7681 gcccgccgat tctcactcca tcgcgcggat ggcgcggaga acttcgccgg gcgaggtaac
     7741 gctgagcagc tcctcgcgaa atgactcgta ctgcagcatc tccgcgaggg ccttgaagac
     7801 cttgaggtag aggttgtctt cgtagggcgg ggcggccatg atgaaaaaaa ggcggaccgg
     7861 cgccgagtcg ggagcgccgt agtcgtagcc ggtatgggaa cgggcaaacg cgatcatgaa
     7921 ctcggtggcc tgcatggagc gaatgtgggg aaaggcgatg ccgtggccga tcgcggtcga
     7981 agcttttttc tcgcggttga caaagtcgat gaggagcttg ttgcggttgc cgacgcggtg
     8041 agtctcgttc aggaggtcga ccagctcgag gaggatcagg gacttggcct gctcccgcca
     8101 gcgttcaatc gactggccct cctcgggcgg atcgatgacg gtctccattt ccagtttgac
     8161 cgcatcttcc gtcatgtagc gcgagatatt catagtaatt ctatcggtcg caaccggtta
     8221 accatgaagg agagtatacg tgacggcctg cgagattgtc aacaggagat ccctccgtac
     8281 cccggagtgc gacgctgccg gtgactcagg ccgtcaccgg gccagttacc cgcttgaggc
     8341 cgggcaaatc aattgatgcc accgacaggt gccggctccc tgtcaatccg atgtcgccgg
     8401 gcataaggtc cgtggcggca ccatagggag gggcggcggc cgccgggtct gtccggtcgg
     8461 ggaaccggca accttgacat tcggtcgggg ggagtgtact ttcccggtac gtcagcggcg
     8521 ccggcggcct gaaccttggg cccgaacgtg ccgataaata ttttgacagc aaggagttaa
     8581 atttctctgg tggtcaaatt ttcggaagtg aaacgcaccc actcctgtgg ggaattgcgc
     8641 cccgctcatg tcggcacaac cgtcaggctt aacggatggg tcaacagtta ccgaaacctg
     8701 ggcgggctgt tttttctgga cctgcgcgac cgctacggca tcacccaggt ggtgatcaac
     8761 ccggaaacgt tcgacaagga catgctggcc gaggcgcagc gtgtccgcca tgagtacgtc
     8821 gtggcggtca cggggaaagt gcggcaccgc ccggagggga cggcgaacga gcgcatgctc
     8881 acgggcggga tcgaggtggt ggccgaggcc ttctggatcc tttcggaatc gaagacgccg
     8941 ccgtttgaga ttgaggagga gacgagcgcg agcgagaagc tgcgcctgga gtaccggtat
     9001 ctggatttgc ggcgggggcc gctgcaggag cgcatccggt tccgccatga tctgaccatg
     9061 tgtgtgcgca aatacatgag cgcgcaggac ttcctggaga tcgagactcc cctgctgatt
     9121 cgctcgacgc cggaaggggc gcgcgactac gtggtgccga gccgcgtcca ccacggccgc
     9181 ttctacgccc ttccgcagtc gccgcagttg ttcaagcaga tcctcatgat cgcgggcttc
     9241 gacaagtatt tccagatcgc gcggtgcctg cgcgacgagg atctgcgcag cgaccgccag
     9301 cccgaacaca cgcagatcga cgtcgagctc tcgttcgcga cgcccgagga cgtgttcgcg
     9361 gtcatcgagg gcctgatggc gaacgtgttc gagaacctgg caggggcggc cgtggcgacg
     9421 ccgtttccgc gctacaccta tgccgaggcc atcagccgct ggggaatcga caaacccgac
     9481 ctccgctttg gcatggagat tgtcgacctc accgagatcg cgggggcgtc ggaattcaag
     9541 gtgttccggg agaacgcggc gcgcggcggc gtgatcaagg ggatcgtgct gtcgggcgga
     9601 gccgcatact cgcgcaaaca gctcgacgag ttgaccgagc tggcgaagca acaaggggcc
     9661 ggggggcttg cgtatattct gaggaccgcg gaaggcgaca aatcgccgat cctgaaattc
     9721 ctgggcgagg cgacggcggc ggccatgagc gcccaggcca aagccgcgac cggcgatgcc
     9781 ctgttcctgg tctccgaccg cccggcgaag actgaagcga tcctcgggca gttgcggctg
     9841 cacctggggc gggcgcaccg gctggcggcc cggaacgagt ggcggttcct gtgggtcgtg
     9901 gatttcccgc tgtttgaatt cgatgaggag tcgcgcaccc tacaggcggc gcacaatatc
     9961 gtatcgcacc cgatggaaga ggatgtgccg ctgatcgagg ccgggtttgc cagcccgctg
    10021 ccggtgtccg accccggcca tccgtggcgg cgggcccggg cgatgcagta cgacctggtg
    10081 ctcaacggct gggaaatcgc ctccggcggt cagcggatca accggcggga tctccagcag
    10141 cggatcctca acatcctggg gatcgacgac gagcgggccg agcggatgtt cggatttctc
    10201 ctgcgggcgc ttgagtacgg tgcgcccccc cacgccggca tcgcggccgg tctcgaccgg
    10261 ctggccgctc tcatgaccgg gggcgagtcc atccgcgacg tgatcgcctt tcccaagacg
    10321 accaacgcga tgtcgctgat ggacggttcg ccggccgaga tcgacccaca ccagttggcg
    10381 gaactggggc tggcaatcag ggaggagcag taggcggacc ggtccgccgt atatgaatgc
    10441 ccatctggac gagagcgtgg tccggtcctt cacgatcgag ggcatcgacc agcgcctgct
    10501 cttcgggcag aacgacgtgt tcctgcgcat catcgaggcg cggttcgcct gccggattgt
    10561 cgcccgcggc gacaagatcg tcatcgacgg cctccccgac gtggtcgaga aggtcgtgcg
    10621 cctgctcaat gacctggtga cgcggatcaa gcagggggac gcggtgaccg agcagtacct
    10681 caactacgcc atcgtcatgg tgaaagagag cgggctcgga ccggcccagg acatttcgac
    10741 gcagtcgctg ctgaccagtt cgctcaagcg cgaaatccgt cccatgacga tcgggcagac
    10801 gaagtacgtg gaggcggtga gcgcgaacga catcgtcttc tccatcgggc ctgcggggac
    10861 cggcaagacc tacctcgcgg tggccatggc ggtggcggaa ctgaaagccg ggcgggtcaa
    10921 ccggattgtg ttcacccggc cggcggttga ggcgggcgag tcgctcgggt tcctcccggg
    10981 ggacatccgg gccaaggtcg acccgtacct gcgcccggtg tacgacgccc tctacgacat
    11041 gatgcagccg gacaagatcc gccgccttct ggagctcggc atcatcgaga tcgccccgct
    11101 cgcgttcatg cgggggcgga cgctcaacga ggccttcgtc gtgctcgacg aagcccagaa
    11161 caccacctcg gcgcagatga aaatgtttct tacgcggatc ggcgagaagt cgaaggcgat
    11221 catcacgggc gacatcaccc agatcgacct gcccgaccgc cgcggctcgg ggctggtgaa
    11281 agtccagggg atcctgaaag ggatcgaggg gatcgcgttc atctacctga ccgaccggga
    11341 cgtgatccgc caccggctgg tgcaggacat catccgcgcc tacgaggccc acgagggccg
    11401 gtcgaagaaa cgctgaaggg cggggcggcc atgctgatca cttcgatact cctgcggatc
    11461 aagcgtgcga tcaagcgcct gctgaaagtg cagagcgaat cacgcctgac catcaccgat
    11521 accgcgcgca gccgcgcgac caaggcggcc atgctcgcgg ccgtggccct ggccgtcggc
    11581 attctctacc cgggggagga gctctacgac ccgttcgatg tcccgcggcg cggcgaggtc
    11641 gcgccggccg acgtggtggc gccgtttgcg atcacggtcg gcaagacgga gcgggagatc
    11701 gaggacgagg aggagatggt ccggctggcg gtgccgttcg tggtcgatta cgactcgacg
    11761 gccgtgcggc aggcgctcgg cgggctgcac cggttcctcg ccgcggccga ctcggtcagc
    11821 cgtctgtcgc gctggcaggc gcgcgtcgat gaaatcgccg acaccctcga ggcgcgctac
    11881 gcgcgcctga cccggagcgc cattctcgac gccctggggc ggggcaacct gcgcgacgtc
    11941 gagacccggc tgcgccgcat ctacgaggag gatatctacc gggtgggcgt gctgccgagc
    12001 aagaactcca tccccgagag ccggaacaaa aacgtgctca tccggcgcgg ggagcgcgag
    12061 aacatccacc accgcgacaa actgctcttc ctggagatgg ccgatgccca gctgctgtcg
    12121 acgctcaacc ggctggcggc gacggagccg atcgatgtcg agttccacta cctcgtcggc
    12181 cgggccttcc tccagcccaa cctccacgtc aacatggccg agtacacgcg gcggctcgac
    12241 gaggagctca cgcagattca gacggtgaag gagacggtcg aagaaggggc ggtggtcgtg
    12301 cgcgccggca gccgggtgaa cgagcggcag gagcgcatcc tccggcagtt ggtgcagatg
    12361 cagcgggcgc gcgcggccga gcagagctgg ctgctgaacc tggtcccggc gatcgcgcgg
    12421 gtcatcctcg tgctcggcgc cttttcgacc ctctttctgt ttctcttcca cttccgccgc
    12481 gaggtgttct actccaatga gcggctcatc gccattctga ccgtctacgc catccagttg
    12541 ttcctcatct acctcctcga ccggtggaac ctctcggtct acctctaccc cgtggctttt
    12601 ctgccgatcg tgctgacaat cctgttcgac gccgaggtcg gggtcctgtc ggccatcgtg
    12661 ctggcggtcc tggtcggcat cgtccaccgg tttgatttca cgctgacgtt catgaccgtg
    12721 gtggcgggga cggtggcggt gttcagcgcg cggcgggtgc ggcggcgggc gcacttcttc
    12781 cgcatcatga tctacacggc cttcgcctac gccgtgttca tcctctttgt cgaaaacctc
    12841 aagctcgcgc cgtcaaccga aatccccgcg ctcatggctt acgggctggc caacgggttc
    12901 gcctgcgtga tcctcgcgat cggcattctc cccgtcttcg agtcggtttt cgggatcacc
    12961 accgacatca ccctcctgga actgtcggac ctgaaccacc cgctcttgaa acggctggcg
    13021 ctcgaggcgc ccggcaccta tcaccactcg atcattgtcg gcaatctctc ggaggcggcg
    13081 gcggaggcga tcggcggtaa ctcgctgctc gcgcgggtcg gcgcctatta ccatgatatc
    13141 ggcaagatgg aaatcccaga gtatttcgtc gaaaatcagc tgtccgtgaa atcgaaacac
    13201 gagtcgctct cgccctcgat gtcggcgctc attctctcct cgcacgtcaa aaaagggcgg
    13261 gcgctcggcg aggccgccga catccccgac gacgtgctga atttcatcga agaacaccac
    13321 ggcacgatgg tgcagaagta tttctacgac aaggccctgc gggccggcaa cggggaggcg
    13381 gtgatggata aattccgcta cccggggccg aaaccgcaga cgcgcgagac ggggatcgcg
    13441 atgctggcgg atgcggtcga agcggcgagc cgcaccctcg accacccctc gcccgcgcgg
    13501 atcgacaacc tcatccagcg catcatcaac gatcgcttcg aatcgggaga actggaccag
    13561 tgcccgctca ccctgcggga tctggccaag atcaagcggg ccttcgccca ggtgctgatc
    13621 gccgccttcc accaccgcgt ggagtatccg aaagacgaga aggccgagga gcgggtgtga
    13681 agctgcggat atacaaggag acgaccgtgc gggtcccgcg cgcgcggctc gagcggctct
    13741 tccgggcggt ggcccggggc gagcggcgga aagcggggga ggtcaacctc gtgttcaccg
    13801 gggggcgccg gatgcgccgc ctgaaccgca cgtaccgcgg gcgggacaag gccaccgacg
    13861 tgctcgcttt cccgctccac gagggggccg gggagcaggc ggacgagatc tacggcgagg
    13921 tctatatcgc cgtgcccgtg gcccgggccc aggccgccgc ctacggggtc gggctgggcg
    13981 aagagatcct gcggctggcc tgccacgggt ggctgcacct tttcgggtac gatcaccaca
    14041 ccccggccga ggccgccgcc atgcgcacgc gggaggagcg ctacctcgcc gcggtgaggg
    14101 gagcctagcc ctatgctgga cctgctctac gttttcgcca tcatcgccgg gtttgccacc
    14161 gcctacgtga tctcgctgtt ctcgctggcc gtctacgtgg acccggagga ggtcgagaag
    14221 ctttccccca accagtcggc ccggcggcgg gggtttgtga agaagctcgc gaacgatccg
    14281 ctcgcgctgg tgcagagcgc cacggtctac aagtcgttcg cgctgatcat cgtcaccgtg
    14341 gccatgtacc agctccttcc ccgcctgggt gcgatttcgg ggtggccgac aactgtcgtg
    14401 cgcctcttcg gcatgctcct gacctggctc ttgtacatct tcttcgtcga gtacatgccg
    14461 aggcgatcgg cggccgcggc cgaccgggcg cggcttttgc ggcacctctg gctcgtcggc
    14521 atcatctact tcctgttctt cccgctcgtc ggacgctacc ggcgggcgct gcagcgcatt
    14581 cgcccgccga aagacgtgac cggcgaggac aaagaggacc tcgtcgagcg ggcgctggag
    14641 acgctcgccg agcaggcggg gatcggcgag cgcattctcg aggaagagaa gaaggaaatg
    14701 atcgggcaga tcttccagct cgaccagacg gtggcccggg agatcatgat cccccgcatc
    14761 gacatcgtgg ggatcgaaaa atcgatgacg tttccggaga tccgccggct ggtcatgcgc
    14821 gacggccact cgcgctaccc ggtctacgag gggagcatcg accgcatcct cggcctcgcc
    14881 tacgtgaagg acatgttcaa ccacctgccc gagccggggg aggagttccg ggtgaccgac
    14941 cacatgcgca aggcgtattt cataccgggg accaaggtca tcggcgaact gctccgcgag
    15001 ttcaagcata agcgcatgca catcgcggtg gtcgtggacg agtacggcgg cgtgtcgggg
    15061 ctggtgacgc tcgaggatat catcgaggaa atcttcggcg aaatccggga cgagcacgac
    15121 tgggagcagg agcagattgt caagctgacc gacggggcct ttctcgtcaa cgccggactt
    15181 ctggtcgagg acctgcagga tttcctggac accgacttcg agcagagcga ctacgacacg
    15241 gtgggcgggc tgatctacga cctggtcggg tcggtgcccc gggaggggca ggtgatccgg
    15301 tggcacgatc tcgaattcga ggtcgagcgc gtccaggggc agcggatcgt cgccgtcaag
    15361 gtgcggcggt aggggacgat cggttcacgg aacgggttat cttgaacagc ggaagccggg
    15421 gccggcgccg cggggcgcct aggccggcgc gggcgggacc gggtcggacg gggcgcggtg
    15481 gtgggtgcgg cacaggcaga gctggcgcag ctggcccacg atctcatggt tgcgaaaggg
    15541 cttgctgatc agcccgtcga cgtccgcctc gcgcaccgcc ttgtccatga gagcgagccg
    15601 ctggccggtg atgaagatca ctttggtgtc ggggaaacgg gaccggatct cgccgagcag
    15661 cgcgactccg tccatgtggg gcatggtgat gtcggtgatg acgatgtcga agggctgctg
    15721 ggccattttg aggagggcgt ccgcgccgtc ggtggccgtc gttgtgtcga agccggccga
    15781 cgaaaggctg cgcaggagta tttgccggat cgccggctca tcgtcgacga ccaggatggt
    15841 ggttttcgct gtgttcacga aaaggccctc aggacgcggc cgcgcctcaa ccggcgccgc
    15901 tatcctgttg tagtatcggc aggtggcgca ggatccttga gtggacagcc agggacatgt
    15961 tcaggcgaaa tgccggtaga cgagaatcac gaggatgagg gtgaagacca cgccgacaag
    16021 gaagccgatg cggaaagtgt tgagccgggc caggcgcttg ggacgggccg ccgggggggc
    16081 gctcaactcc gagtggcagt gcttgcactt gaccgccccc tccaggatgg tctccttgca
    16141 gaaggggcag cgggtgaccg gtttggttgc cagcgccatg ccccatgggt agcggagcgc
    16201 gcgccgcgag gcaacaaaaa acagccgggc ccgggcgccc cttactcctt ctccggatcg
    16261 acaaaacgca cgcccgactc aaaaccgatc ggacctttgc ccccgccggg gaaaccgaaa
    16321 gtcttgatct cgccgaactg cgcctcaaac ttcttgaggt tctcagtcag ggcctggtgg
    16381 aggagtttgg cgtgcatggg ggtcatgatg atgcgcgaga ggaccttggc cttcggcagg
    16441 cggggcatca cgcgggcgaa gtcgatgatg atttcggtgg gggagtgcat gatcagggcg
    16501 aggttggcat aaatgccctc ggcggccttc tcatcgaact ccacggcgat ttgctgctgc
    16561 ggcggctgct gctgcatcgg gaggctcctt tccgcggact tcaaagtgct tgtgcatgat
    16621 caagcatggt aatatattcc gccggcaagt caacagagaa gcggcggaaa gggccgccgc
    16681 ggaccgatcg cggccaagag gaggcattgc ccgtgtcgtt gagtgcggaa cgcatagcgg
    16741 aagtgatcgc gaggctgggg aaggcacggg tgctcatcct gggggatatc atgctcgacg
    16801 agtacctctt cggacgcgtc gaccgcatct cgcccgaggc gcccgtgccg gtcgtggaaa
    16861 tctcgcacga caagctcctg ctggggggcg ccgccaacgt ggccgcaaac atccgggcgt
    16921 tgggcgacga accgctcctc gtcggggtgg tgggtgaaga cgaggcggcg gtcaaactca
    16981 gccaactgct caaacagcac ggcatcgggg ccgattactg cgtgtgcgac cggtcccgcc
    17041 agaccaccat caagacccgc atcatggccc acagccagca ggtggtgcgg gccgaccggg
    17101 aaaaccgcca cgaactccac cccgacgtgg aacggatggt gctggccaag ttcacggccg
    17161 ccgcggagtc ggtcaacgcc gtgattatct ccgactacgg caagggcgtc attacgctgt
    17221 cgctgctgaa cagcgtgatg gaggtttgcc ggaagcgggg gctgtttgtg gccgtcgacc
    17281 ccaaagaaac gcatttccac aactataaac gcgtgtcgct tatcaccccg aaccaccacg
    17341 aggccgggtt cgccgccggg cggcggatag tatccgaggc cgacctgatc gatgtcggga
    17401 acagcctgct gcgggaactc gaggcccggt cgattctcat cacccgcggg ccggacggta
    17461 tgtcgctgtt cgagcaggac gccgaaccga cccacatacc gaccttcgcc cggaaagtgt
    17521 tcgatgtgac cggggccggc gataccgtga tcgcggcttt cgtggccgcc gtgtgcgcgg
    17581 gggcgacgct ggtcgaggcg gcgattgtcg cgaacgccgg ggcgggcatc accgtcggag
    17641 aggtcggaac cgcgaccgtg tcctctgcag agctctacgc ggaactgctg cgcaacgtca
    17701 agaacgggac gctggtgacc aggggatcga cggggacgca ggcgtagcac gggagaaagg
    17761 tccgacgggc ggcgcaatga acagggccag gccgacaggg cgcaggagga tcggcgaggt
    17821 ggcggcccgc caccggcggc gcggccacaa aattgtcttc accaacggcg tgttcgatat
    17881 cctccaccgc gggcacgtgg agtaccttca aaaagccaag gctatggggg acatactgat
    17941 agtggggctg aactcggatg cttcgacgcg ccgtctcaaa ggaccggaac ggccggtcca
    18001 gagtcaggcg gatcgggccg ccgttctcct ggccctccgc gctgtcgatt acgtcgtcat
    18061 tttcggcgag gacacgccgt acgaattgat caggcaggtg cggccggatg tcttggtgaa
    18121 gggatcggac tacaagttgg gcgaaatcgt ggggagggat atcgtgcggt cgcacggcgg
    18181 caccgtgcgg cgcgtgaaac tgacgccggg gcggtcgacc acgcaggttt tgcggaaagc
    18241 cgcgaaaggg tagaggcggg agtcgggagg actcccgccg cgccggtttc agcagccggc
    18301 cggggccgga ccgccgcgaa atacgtacgc gatcagggac gtgacatcga cgaccgtgac
    18361 gtgcccgtcg ccggacacgt ccgcttcagt gaggcagtag ggaggcggtc cgccgcggaa
    18421 aacgtatccg atgaggaacg ttatatcggc cacggtgacg ccgccgcttt cattcatgtc
    18481 tccgcgcagc aggcagcagg aggcggggta tttgtagaga acgatgtcct cgagccattc
    18541 ggtgccggat acctgctgcg gcacctcccc gatcacgtac acgttgccat cctggtcggc
    18601 ggcgagatcc cggaaggtcc ctaagcccgc tgagtctacc gcgaagggac gccaccagag
    18661 caggtcgccc tcgggagagt atttcacggt atggggccag cagggggagt aaccgaagac
    18721 agcgttttgg ttcgcatcga cagccacgga cgtaccgccg ctctcgcacc catcacccgt
    18781 gggcgacggc gagtagaacg tgcgcgccca tcgctcgttt ccatcggaat tgagtcgcac
    18841 tccgaccagg gatcgaggat cgtacccaaa gaagtcgtag tcacggacga tgctcaacca
    18901 gacggaaccg tctccgccga cgtgcaggtc accgatgtaa tcgcgcagac cggcagggcc
    18961 gctgtaggtc cagtcccacg gaaaaacgcc cgccgcgctg tatttgatga agatgcagtc
    19021 cgcggtttcg tactccccga gctccccggc gacgtacacg cttccctcgt cgtccgttcc
    19081 cacactgagt gcat
//
LOCUS       DAOPOE010000061        18971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3529_length_18971_cov_4.319095, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000061 DAOPOE010000000
VERSION     DAOPOE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18971
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3529_length_18971_cov_4.319095"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..513)
                     /locus_tag="PKM94_09045"
     CDS             complement(139..513)
                     /locus_tag="PKM94_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD66823.1"
                     /translation="MRRSHTLLGGILPVFLSLGVFGGGKGNVDPDLWSRDEITGTVSD
                     SATTDPIAGAAVFLLGTALPVSSATSDSSGRYRIYPESFGETQLLCEKNGYVSDTQLV
                     NLVPDKFKYEVDFLLVRDSSAR"
     gene            complement(1007..1873)
                     /locus_tag="PKM94_09050"
     CDS             complement(1007..1873)
                     /locus_tag="PKM94_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOD66824.1"
                     /translation="MTTFPILAVVIFFALILLFNMIKVLKEYERGVIFRLGRLIGAKG
                     PGLIILIPIVDKMVRVDLRVITYDIPSQDVITNDNVSVKVNAVVYFAVVDPNKAIVSV
                     ANFFEATSQIAQTTLRSVLGQFELDELLANRDKINAQLQHIIDEQTEPWGVKVSVVEV
                     KNVDLPPEMTRAIARQAEAERERRSKVIHAEGEFQAAQKLADAATVIGAAPGAMQLRF
                     LSTLVEVSAERNSTLIFPVPIDLLTSFKSFLDWNSGPKQTGAASIEAFRKAMENLGGP
                     SGPKPDAGRDKG"
     gene            complement(1879..3315)
                     /locus_tag="PKM94_09055"
     CDS             complement(1879..3315)
                     /locus_tag="PKM94_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOD66825.1"
                     /translation="MRLRLVLIAAVWMLASALLLSNSLAQQEDRPPLGAGEDSVLVPP
                     EARKALREIHSSVPPYAMIPDSVLRSQSSAEGRPPLVYVMVLEGPIGAVASDRVEEAV
                     AQAEEKGAELLVIELDTPGGFTQSTWTISKAIMNSRVPVAVYIAPRGARAGSAGVYIT
                     YAAHIAAMAPSTNIGAAHPVGSGAVDSIMNEKITNDAVAQIKAAAKERGRNVEWAEQA
                     VRQSVSVDDREALELNVVDLRADDLDDLLARINLRIVALPDAVDTLFLTDARVEEITT
                     SFVYRLLQIITDPNVALILFSLGSLGLVIELYNPGAILPGVVGAICLILSLYAFQTLD
                     VNYAGLALIILAIILFILEIKVVSYGVLTLGGLIAFFIGGLMLYDTVDPTLRISLSVL
                     ITAAVCVAAVVLVVGYLVYKAHRRQVFVGEEGLIGKTATVRAAGMVYVDGALWRAVSE
                     GTLEVGDKVTIVRTDGLTLHVKKLEGQR"
     gene            complement(3478..4644)
                     /locus_tag="PKM94_09060"
     CDS             complement(3478..4644)
                     /locus_tag="PKM94_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66826.1"
                     /translation="MRLLLAYSWRNVFTRRLTSALTIFGVALVVFVFCAVLMLSHGLR
                     RTLVDTGDDNNAVVIRKAAQTEITSIVYRREGNIIKADPAVALTPEGRPRASGELLVL
                     ITKTKRSSDETSNVPVRGVAPEALLLRPDISLVAGRLWRPGRSEIIAGAKVAAGFQNA
                     GLGEKVRFASRDWTVVGIFEDNNSGFESELWGDYDQLAAAFERPIYSSVTVRLKSPDR
                     FSGMKARLENDPRLAVDVRREKQYYADQSSFTATYINILGLVISVIFSVGAIVGAMIT
                     MYGSVAGRTREIGTLRTLGFGRFAILSAFLAESVLMALVGGGLGVTAAALLRFAQVST
                     TNWDTFAELAFSFETSPGIIAGALVFAALMGVVGGFLPAVRASRLSVLSALRAK"
     gene            complement(4654..5811)
                     /locus_tag="PKM94_09065"
     CDS             complement(4654..5811)
                     /locus_tag="PKM94_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66827.1"
                     /translation="MSVLRLIFRNAGRHKLRTFLTVLGLGIAVMAFTVIRSTIDAWYA
                     GAEAASPNRLITRHAVSLTFFLPLSYADRIRKIEGVTAVSHASWFGGIYVNPKNFFAQ
                     FAIDPASYLEMYPEFIVPEDQREQFRRQRNGALVGRRLADRFGWSVGDAVRLTGTIFP
                     GDWDFTIVGIYTGARENTDENSWFLRWDYIDERLRRETPVRAGYVGLFAVQIDDPGRA
                     AEISGLIDGAFRNSSSETKTETEEAFNLGFVAMAGSIVLGLNVISLMVIGIIMLVLGN
                     TMAMTARERINEYAVMKTLGFRTPHIVGLIVGESLVIAALGGALGIFLTWPIGNLVRT
                     ALSAFFPVFATGLGTYALGGAAAVAVGLLAAVFPALKALHTPIVEGLRVVD"
     gene            complement(5808..6488)
                     /locus_tag="PKM94_09070"
     CDS             complement(5808..6488)
                     /locus_tag="PKM94_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD66828.1"
                     /translation="MPNALVRLENISKSYWRDALEIPVLRNLTFEIPEGEFLALMGPS
                     GSGKTTLLNLIAGIDRPSGGRLFVGREEISQMSEAALARWRSRTIGFIFQFYNLIPVL
                     TAFENVELPLFLTDLRRSERRRQVETALSVVGLADRLHHYPNQLSGGQEQRVAIARAL
                     VTDPDLLLADEPTGDLDKQSAEDVMHLLAFLNREFGKTIVMVTHDPRAAGEAHTVLRL
                     DKGELAAA"
     gene            complement(6497..7693)
                     /locus_tag="PKM94_09075"
     CDS             complement(6497..7693)
                     /locus_tag="PKM94_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD66829.1"
                     /translation="MSESDGRPDIGSLRIDRSRKYRDRPRRSVGRWIAWAAILAALVA
                     GYFALREFIQPTLKVRTAAATLLTGSEASADLVATGYVVAQRSAEVASKGTGRLARLD
                     FEEGDRVRVGQVIAELDNADIRAQREQARAELQAAEVDTLTAGRQHRRVRELFASGAV
                     TQIDAEGAESAYLTALARLAAARAALAAAEVALENTYIHAPFDGTILTKNADVGEIVA
                     PFASSASSKGSVVTLADMNSLEVEADVSESNIQKIVVGRPAEIVLDAYPAVCYPARVK
                     KIVPTADRARATVLTKVAFDSLDSRVLPEMSARVSFFLETPAEGAPPRAPVLVVPKDA
                     LTVRDGRTVVFVVGPDNYVAAVPIDAGRELNDYTEVKGGLTEGAVVVLDPPGKLQTGR
                     KVEITY"
     gene            complement(7844..8155)
                     /locus_tag="PKM94_09080"
     CDS             complement(7844..8155)
                     /locus_tag="PKM94_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOD66830.1"
                     /translation="MPAPTKEQIMEVLRPIYDPEIRLGIVDLGLVYDVVVHDGGKVEV
                     KMTLTTPACPYGEILVAQVHREVAALEGVREVEVLLVWDPVWDPETMASDYAKDVLGI
                     W"
     gene            complement(8155..8631)
                     /locus_tag="PKM94_09085"
     CDS             complement(8155..8631)
                     /locus_tag="PKM94_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOD66831.1"
                     /translation="MTNLDDMYREVILDHFRSPRGKKPITQPDVASHGHNPACGDELS
                     LALEMADDTVKSVHVDCKGCAISTASGSILAEVIKGRSLEEAKRIAGIVRKMLKGEET
                     EIPDDLGDVEALRGVCNFPVRVKCALLAWVTLIEGLRQYEQGRPADPPSVSTEESE"
     gene            complement(8628..9896)
                     /locus_tag="PKM94_09090"
     CDS             complement(8628..9896)
                     /locus_tag="PKM94_09090"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOD66832.1"
                     /translation="MKPHAAAPSAAPLTALDIERLRADFPILHTTVRGRRLVYLDNAA
                     TTQKPNAVIEAESDYYRTSNANIHRGLHHLSERSTELYERTRQHTARFIGGVDPQEII
                     FTRGTTESINLVAYTWGEENLREGDEIVITQMEHHANLVPWVELARRKRAVLRQIPIQ
                     PNGTLDLASLDRLITPRTRLTAVCHMSNVLGTINPVSAIAAAAKRYGAVVLADGAQAA
                     PHLPVRIGELGVDFYAFSAHKMLGPTGVGVLWGRRALLEAMPPFITGGEMIREVSLDR
                     VTWADLPNKFEGGTPNIAGVIAFDAALSYLEALGMDRVRRHEEDLTRYALDRLQTLAG
                     LEIQGPTDPARRGGAISFTDPDLHPHDISTFLDSRGIAIRAGHHCAQPLMRLLGKIAT
                     ARASFYIYNDEADVDALVDALLEMRRYFGL"
     gene            complement(9921..10232)
                     /locus_tag="PKM94_09095"
     CDS             complement(9921..10232)
                     /locus_tag="PKM94_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOD66833.1"
                     /translation="MAPYHKIAAVADIPPGTMKAYRVGYADVLVCNVGGRIYAVANEC
                     THDGAPISTGRLFKEQVVCPRHGARFNVATGAVEAPPAILPLDTYEVKVEGEDIYVAV
                     E"
     gene            complement(10236..11570)
                     /gene="sufD"
                     /locus_tag="PKM94_09100"
     CDS             complement(10236..11570)
                     /gene="sufD"
                     /locus_tag="PKM94_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOD66834.1"
                     /translation="MSTTPTLDTAGAEHAIPDFAPGLARGPRWLRDARRSAAQIFNAA
                     PFPRRGLHLWRYTDPAAFLAPHGSAADHPPVEDRDAIIGLEKQHVDSGALAALAVDYA
                     GREISTYGADRLARRGVVIQPLSLAVDQHGDLVEQHLYRLVGPDLGKFEALNAALWND
                     GIFVYVPEGETIDRPIHLFREGGDRSSVYFPRLLVIVGRNAEITLIDEYAGGSEDVSG
                     GVTHVNGAVEISAGDDSRVRYVSVQRHGAGVNMYLTHRAEIGRDARVLTVPLAIGGNI
                     TKQNFGVLLAGRGADSRLRGMLFGSGGQHFDNHTLHAHGAGNSTSTIDFKTVLRDRAV
                     SAYTGLIRIEQHAKTCEAYQENRNLLLNRGPKAETIPELEILNEDVKCTHGATIGPID
                     PESVFYLASRGIPRDVAVRMIVSAFVEPTLRQIPDDLRGRLAEFIERRLEGL"
     gene            complement(11573..13009)
                     /gene="sufB"
                     /locus_tag="PKM94_09105"
     CDS             complement(11573..13009)
                     /gene="sufB"
                     /locus_tag="PKM94_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOD66835.1"
                     /translation="MAETAKQNQNAGLAALNEDYAAKYGFRDPVDYFHKGARGINHEV
                     VEMISRMKGEPRWMADRRHEALDIFLAKPMPRWGNTALLNEIDFANIFYFMKPIEKKG
                     ETWDDVPEYIKKTFDRLGIPEAEKKFLGGVSAQYESEVVYHSMKKDLIDQGIIFLDMD
                     SGLREHEAVVRQYFGSVIPSHDNKFAALNTAVWSGGSFIYVPPGVDVKIPLQAYFRIN
                     AENMGQFERTLIIADKGSRVHYIEGCTAPVYSTDSLHSAVVELIALEGAYIRYTTIQN
                     WARNIYNLVTKRATAHARATVEWVDGNIGSRLTMKYPCIQLVGEGARGEILSVAFAGI
                     DQHQDAGAKVIHAAPHTSSRITSKSVSKDNGRASYRGLVKIHKNAAHAKVSVECDALL
                     IGAQARSDTYPTMEIDHDQVRVEHEARVSKVAEEQLFYLTSRGLTEDEARLLIVNGFM
                     EPFTKELPMEYAVELNRLIELEMEGSIG"
     gene            complement(13013..13783)
                     /gene="sufC"
                     /locus_tag="PKM94_09110"
     CDS             complement(13013..13783)
                     /gene="sufC"
                     /locus_tag="PKM94_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOD66836.1"
                     /translation="MSDHTPLFSFKDVHVEVDGKEVVRGVSLTVRPGEVHAIMGPNGS
                     GKSSLSLALMGHPSYRITAGEAWLEGRNLLEMEADERSRAGLFLAFQYPLAIPGVTVA
                     NFMRAAVQAHRGKGADMSDFRKVFLAHMAELGIDRQFAARYLNDGFSGGEKKRIEILQ
                     MTMLKPAMALLDETDSGLDIDALKMVAEGINRFHTERNGVLLVTHYQRLLNYVKPDYV
                     HVMSRGRLVKSGGPDLALELEAKGYDWLVGPSAAVAEV"
     gene            complement(13802..14353)
                     /locus_tag="PKM94_09115"
     CDS             complement(13802..14353)
                     /locus_tag="PKM94_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD66837.1"
                     /translation="MRISSLEEYGLRCLLALARRGADGQLSISEIADLEGLSVPYASK
                     LLAILRKAGLVTAVRGRSGGFCLGREPERITLLQVMTALGGPIIDPDHCARFSGRREV
                     CVHQDDCSVHHVLDTLAAYIADILGRTTLQDLVARSAGRPAGPGLIGAEQLLGGAAAT
                     TDITPRPAEIDGLARDTQSQQSL"
     gene            14656..15147
                     /locus_tag="PKM94_09120"
     CDS             14656..15147
                     /locus_tag="PKM94_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOD66838.1"
                     /translation="MAIAVGVTAGAIDRWTIFRLSWHFGFMQFGMPIVGWMAGSYLTG
                     WVGSFGRWLAAAVLFAIGARLIWEQANPEVRKWKGDPTRGASLLVLMVATSIDALAAG
                     LSLALVGVAILYPSMVIGVVAAGMTVLGLAFGHAVGMKLGRIAGVIGGVILIALAVRA
                     VVG"
     gene            complement(15176..15556)
                     /locus_tag="PKM94_09125"
     CDS             complement(15176..15556)
                     /locus_tag="PKM94_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66839.1"
                     /translation="MPVEKSEPPKAAPFVGRYVGASAFTPADPADSAYAETVDVWFIF
                     DAKAYVYGEGDTIPEDEAGDGGGPYRWTDSTVTLEGAYPKILRPAIVLRGAFTWRVTG
                     DTLFLSQPSAAGAAAGYRLRLVRQ"
     gene            15814..17601
                     /gene="iorA"
                     /locus_tag="PKM94_09130"
     CDS             15814..17601
                     /gene="iorA"
                     /locus_tag="PKM94_09130"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOD66840.1"
                     /translation="MRELLSGNEAVARGAFEAGVRLAAAYPGTPSTEILETLAERYPS
                     IYSQWSPNEKVAYEVGIGASLGGARTLVAMKHVGLNVAADPFMTHAYTGVNAGFVIVS
                     ADDPEMHSSQNEQDNRHFAKFAQVPMLEPSDSQEAKDMTVTALELSEVFDTPVLLRVT
                     TRISHSKGIVELSEAVKGPDKKFVKEPSKYVMIPANAKKRHVAVAERLARLRRFSEET
                     PMNTVEENGSRVGIITGGVSYQYAKEVMPEADYFKLGMSYPLPLEKIAAFVRAHEQAI
                     VIEELEPFYEEQIRAAGIAVKGKEYFGVQGELSPHRVAEGLFKAGVIEQPRAAEIPPA
                     ADMFPRPPVLCPGCPHRGAFMALRKLGVAVTGDIGCYTLGALQPLKALDTCICMGASI
                     GNAIGMEKVQGFGKGTVAVIGDSTFLHSGVTGLMDAVYNHSNVTVIILDNRATAMTGG
                     QMHPGTGRTLMGEPAHEINIGQLVRALGVKNYREADAYDYEQMQEVIREEIDKPGPSV
                     IHTTRPCVLMPKRIMDEPYLVDLDLCNGCGVCFKLSCPAIAAAAQLNERGRPKATIDI
                     TLCTGCTLCAQVCPTEAIQLQSQFAKEGA"
     gene            17604..18206
                     /locus_tag="PKM94_09135"
     CDS             17604..18206
                     /locus_tag="PKM94_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOD66841.1"
                     /translation="MADDRFNILIVGVGGQGVLLASEIISEVAMQAGFDVKKSEVHGM
                     SQRGGVVSSHVKIAPKVYSPTIGYGQADVVMAFEQAEGLRAVDWMKGDGVAIVSETTL
                     IPAIVTSSKEFTYPKDAIGAMRKVARRVIGVPADKIAGELGNPRLVNTILLGVLSNYL
                     PFDLDLWKEAIRARVKEKFVPVNLAAFDRGRAVKLAPVHA"
     gene            18222..>18971
                     /gene="buk"
                     /locus_tag="PKM94_09140"
     CDS             18222..>18971
                     /gene="buk"
                     /locus_tag="PKM94_09140"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOD66842.1"
                     /translation="MAEKLLIINPGSTSTKVALFEGDRRLAEETVRHDPARLRAFDNV
                     AEQFAFRMETIDPWIAALGLAAGEIAAVVGRGAPLRPLEGGSYRITPQMLDDVRTMRY
                     SNHASNLGCLIAHHLGLRYGVPAFIADPITVDNFTDIARVSGVPEIERKCRVHALNIK
                     EVSRREAEKMGMTLATAAFVACHMGGGVSVAALRGGRVIDVNDALLGMGPFSPDRAGA
                     LPIGELVRLCYAGKYTEKEMIDKLSKHSGLAA"
BASE COUNT         3270 a         5645 c         6821 g         3235 t
ORIGIN      
        1 atatctcacc tccttccacc gtcggtttcg aatacgcaag gctgctgcca tccggagacc
       61 acgacggata ctgagcacct tcacccatga ttctcagcag gttcttgccc tcgaccatca
      121 atccccagat cgctccggtt accgcgccga ggagtcccgg accagcagga agtctacctc
      181 gtacttaaac ttgtcaggta cgaggttgac cagctgtgtg tccgatacat acccgttctt
      241 ttcgcaaagc aactgcgttt ccccgaagct ctcggggtat atccgatatc tcccgctcga
      301 atcagaagtt gccgaagaca ccggtagcgc cgtgccgagt agaaaaacag ccgcaccggc
      361 gatcgggtca gtggtcgcgg aatcggagac ggtgccggta atctcgtccc gcgaccacag
      421 atccggatca acgttaccct tacctccacc gaagactccc agtgacagga agaccggcaa
      481 gataccgccg agcaacgtgt gacttctacg catccggaat cccgtgtgag gctcctgtga
      541 gcaactaccc tcaaattcat atatactgtg tctgtgcgcc tgtcaaggac aatttcggta
      601 gtgggtcagt ttgaataaga acacgataga atcgatcggc tatgccccat ccgctcctac
      661 ttcccgcacc cgattcgtgc ctcgacctcc ccgttcatcg ccatctccaa cgtccccttc
      721 accgatggct tcacttcccc ctgaatcaac tcccacatgt tcgtgacacc cagccaccga
      781 cagaatagac ttttctctga gaagtccact tctcgcatgg gcatgtcctc agtaacttcc
      841 aggttttcat ggacatgccc tcttcttcat taccttccat agaaattaca caagagaagt
      901 taggctactc gagagcggca agccggccaa taaaaggggc aggcttaccg agcctgcccc
      961 gtagtttgct ctgccgacaa agcggaacaa atggcccggg agtgcgctaa cccttgtccc
     1021 gcccggcatc aggcttcggc cccgatggtc cgccgagatt ttccatcgct ttgcggaaag
     1081 cctcaatcga cgccgccccg gtttgcttcg gtccggaatt ccaatcgaga aagctcttga
     1141 aggaggtcag caggtcgatc ggcaccggga agatgagcgt ggagttgcgc tcggccgaga
     1201 cctcgacgag ggtcgaaagg aaacggagtt gcatggcgcc cggggcggcg ccgatcacgg
     1261 tagcggcatc ggccagtttc tgcgcagcct ggaactcgcc ctcggcgtga atgactttcg
     1321 agcgccgctc gcgctcggcc tcggcctggc gggcgatggc gcgcgtcatc tcgggcggga
     1381 ggtcgacatt cttgacctcc accacgctca ccttgacgcc ccacggctcg gtctgctcgt
     1441 cgatgatgtg ctgcagctgg gcgttgatct tgtcgcggtt ggccagcagc tcgtcgagtt
     1501 cgaactggcc gaggaccgag cggagggtcg tctgcgcaat ctgcgaggtg gcctcgaaga
     1561 agttggcgac gctgacgatg gccttgttcg ggtcgacgac ggcgaagtag acgacggcgt
     1621 tcactttcac cgagacgttg tcgttggtga tgacgtcctg ggaaggaatg tcataggtga
     1681 tgacgcgcag gtcgacgcgc accattttgt cgacgatggg gatgaggatg atcagaccgg
     1741 ggcccttggc gccgatgagg cggccgagcc ggaaaatgac gccccgttcg tactccttga
     1801 gcaccttgat catgttgaac agcaggatca aggcgaaaaa gataacgacg gccagaatgg
     1861 gaaacgtggt catacaactc acctctgtcc ttcgagtttc ttcacgtgca gagttagtcc
     1921 gtcggtgcgg acgatggtca ctttatcgcc aacttcgagc gtgccctccg acacggcccg
     1981 ccacagagcg ccgtcgacat acaccatgcc cgcggcgcgg acggtcgcgg tcttcccgat
     2041 caggccctcc tcgccgacga acacctgccg acggtgggcc ttgtagacga gatagccgac
     2101 gacgaggacg acggcggcga cgcacacggc tgcggtgatc aggacggaca gcgagatgcg
     2161 cagcgtcgga tcgacggtat cgtagagcat gagtcctccg atgaaaaagg cgatcaggcc
     2221 gcccagggtg aggacgccgt aggacaccac tttgatttcg aggataaaga ggatgatcgc
     2281 caggatgatc agggcgaggc cggcgtagtt gacgtcgagc gtctggaagg catagagtga
     2341 gaggatgagg cagatggcgc cgaccacgcc ggggaggatc gcccccgggt tgtacagctc
     2401 gatgaccagc ccgagcgagc cgagggagaa gaggatgagg gcgacgttcg gatcggtgat
     2461 gatctggagg aggcggtaga cgaaggaggt cgtgatctcc tcgacgcggg cgtcggtgag
     2521 aaacagggtg tcgacggcat cgggcagggc gacaatgcgg aggttgatcc gggcgagcag
     2581 atcgtcgaga tcgtcggcgc ggaggtcgac gacgttgagt tcgagggcct cgcggtcgtc
     2641 gacggagacc gactggcgga cggcctgttc agcccactcg acattgcggc cgcgctcttt
     2701 ggcggcggcc ttgatctggg cgaccgcgtc gttggtgatt ttctcgttca tgatcgagtc
     2761 gacggcgccg ctgccgacgg gatgggcggc gccgatgttg gtcgaggggg ccatggcggc
     2821 gatgtgggcg gcgtaggtga tgtagacgcc ggccgacccg gcccgcgccc cgcgcggcgc
     2881 gatgtacacg gccaccggga cccgcgagtt catgatcgct ttggagatcg tccaggtgga
     2941 ctgggtgaag ccgccggggg tgtcgagctc gatcaccagc agctcggcgc ccttctcctc
     3001 ggcctgcgcc accgcctcct cgacgcggtc gctcgcgacg gcgccgatcg gtccctcgag
     3061 gaccatgacg tacaccagcg gcgggcggcc ctcagccgac gactgcgacc ggaggaccga
     3121 gtccgggatc atcgcgtagg gcggaacgga gctgtggatc tcccggaggg ccttgcgcgc
     3181 ctcgggcggc acgaggacgg agtcctcgcc ggcccccagc ggggggcgat cctcctgctg
     3241 cgccaacgaa tttgacaaga gcagggccga ggccagcatc cacactgcgg caatgagaac
     3301 caacctgagt ctcatggctt aaatatgctc ctgctgagaa tctgtgtcaa tctaaagaaa
     3361 gtacgggcgg gagcgggtat tattcaatgg aatcggtcgg ggcaaggcgg gcgagggcgc
     3421 cggcggccga ggggcgggga cgattcgtgt ccgtcttcgg cggcgggcgg tgcgccccta
     3481 cttggcgcgc agggccgaca gcacgctcag gcgggaggcg cgcacagccg ggagaaaccc
     3541 gcccaccacc cccatgagtg cggcaaagac gagcgcgccg gcaatgattc cgggggaggt
     3601 ctcgaaggag aaggccagct cggcgaaggt gtcccaattg gtcgtggaca cctgggcgaa
     3661 gcggagcaaa gcggcggccg tgacgcccag cccgccgccg acgagggcca tcagcaccga
     3721 ctcggcgagg aaggcgctga ggatggcgaa ccggccgaag ccgagagtgc ggagggtgcc
     3781 gatctcgcgg gtgcggccgg cgaccgagcc gtacatggtg atcatggcgc ccacgatcgc
     3841 cccgacggag aagatgacgg agatgaccag gccgaggatg ttgatgtagg tggcggtgaa
     3901 gctcgactgg tcggcgtagt actgcttttc gcggcgcacg tcgacagcga ggcgggggtc
     3961 gttctcgagg cgcgctttca tacccgaaaa ccggtcgggg gatttcaggc gcacggtcac
     4021 ggacgagtag atggggcgtt cgaacgccgc cgcgagttga tcgtagtcgc cccagagctc
     4081 ggactcgaag ccggagttgt tgtcttcgaa gatgccgacg acggtccagt cgcgggaggc
     4141 gaagcggact ttttcgccaa ggccggcatt ttggaagccg gcggcgactt tggccccggc
     4201 gatgatctcg gagcggccgg ggcgccagag gcggccggcg acgagcgaga tgtcggggcg
     4261 caggaggaga gcctccggcg ccacgccgcg gacgggcacg ttgctcgtct cgtcggagga
     4321 ccgtttggtc ttggtgatga gcacgagcag ttcgccgctg gcgcgggggc ggccctcggg
     4381 ggtgagcgcg acggccgggt cggccttgat gatgttgccc tcgcggcggt agacgatcga
     4441 ggtgatctcg gtctgggcgg ccttccggat gacgaccgcg ttgttgtcgt cgcccgtgtc
     4501 gaccagcgtc cggcgcagcc cgtgggagag catgagcacg gcgcagaaga cgaagacgac
     4561 gagggcgacg ccgaagatgg tcagggccga ggtgagacgg cgggtgaaga cgttgcgcca
     4621 cgaatatgcc aacagcagcc gcatgggaac gcctcagtcg acgacccgga ggccctcgac
     4681 aattggagtg tggagcgctt tgagcgcggg gaagacggcg gcgagcagtc cgacagcgac
     4741 ggcggccgcc ccgcccagcg cataggtgcc cagcccggtg gcgaagacgg ggaagaacgc
     4801 cgagagggcg gtccgcacga ggttgccgat cggccaggtg aggaagatcc ccagcgcgcc
     4861 gcccagggcg gcgatgacga gcgactcccc gacgatgagg ccgacgatgt ggggggtgcg
     4921 gaagccgagg gtcttcatca cggcgtactc gttgatccgc tcgcgcgccg tcatggccat
     4981 ggtgttgccg aggacgagca tgatgatgcc gatgaccatg agggaaatca cgttcagccc
     5041 gagcacgatc gaaccggcca tggcgacaaa cccgagattg aacgcctcct cggtttcggt
     5101 tttggtctcg gaggaggagt tgcggaaagc gccgtcgatc aggccggaga tttcggcggc
     5161 gcggccggga tcgtcgatct ggaccgcgaa gaggccgacg taaccggccc gcacgggcgt
     5221 ctcgcggcgc aggcgttcgt cgatatagtc ccagcggaga aaccacgagt tctcatcggt
     5281 gttctccctg gccccggtgt agatgccgac gatggtgaag tcccagtcgc cggggaagat
     5341 cgtgccggtg aggcgcacgg cgtcgccgac cgaccagccg aagcggtcgg ccagccggcg
     5401 gccgacaagc gcgccgttgc gctgccgccg gaactgctcc cgctggtcct cgggaacgat
     5461 gaattcaggg tacatctcga ggtaggaggc ggggtcgatg gcgaactggg cgaagaagtt
     5521 tttggggttg acgtagatgc cgccgaacca ggaggcgtgc gagacggccg tcacgccctc
     5581 gatcttgcgg atgcggtcgg cgtaggagag ggggaggaag aaggtcaggg agacggcgtg
     5641 gcgggtgatg aggcggttgg gcgacgcggc ctcggcgccg gcgtaccagg catcgatcgt
     5701 gctccggatg acggtgaagg ccatcacggc gatccccagg ccgaggacgg tgaggaaggt
     5761 gcggagcttg tggcgcccgg cgttgcggaa aatcagcctg agaacgctca tgcggcggcg
     5821 agctccccct tgtccaggcg gaggaccgtg tgcgcttccc cggcggcccg cggatcgtgc
     5881 gtcaccatga cgatcgtctt gccgaactcc cggttcaaaa acgccagcag gtgcatgacg
     5941 tcctcggccg actgcttgtc gaggtcgccg gtcggctcgt cggcgaggag aaggtcgggg
     6001 tcggtgacaa gggcgcgggc gatggcgacg cgctgctcct gaccgcccga gagctggttc
     6061 gggtagtggt ggaggcggtc ggcgagcccg accaccgaga gggccgtttc gacctggcgg
     6121 cggcgttcgc tcctgcgaag gtcggtcagg aaaaggggaa gctcgacatt ctcaaaagcg
     6181 gtgaggacgg gtatgaggtt gtagaactgg aagatgaagc cgatggtgcg actgcgccag
     6241 cgcgccaggg cggcctcgga catctgcgag atctcctccc gcccgacaaa gagccgcccg
     6301 ccggaggggc ggtcgatccc cgcgatcagg ttcagcaggg tcgtcttgcc tgagccggag
     6361 ggtcccatga gggcgaggaa ttccccttcc gggatctcaa acgtgaggtt gcgcaggaca
     6421 gggatctcca gcgcatcgcg ccagtacgat ttggagatgt tttccaggcg cacgagggcg
     6481 ttcggcatgg ggacccctag tacgtgattt cgaccttgcg gcccgtctgc agcttgccgg
     6541 gcgggtccaa aacgacgacc gcgccttcgg tcaagccgcc tttcacctcg gtgtaatcgt
     6601 tcagctcgcg cccggcgtcg atcgggacgg cggcgacgta gttgtcgggg ccgacgacaa
     6661 acaccaccgt ccggccgtcg cggaccgtga gggcgtcctt gggcacgacg aggacagggg
     6721 cgcgcggcgg cgctccctcg gccggcgtct cgaggaagaa actcacgcgg gccgacatct
     6781 cgggcagcac gcggctgtcg agcgagtcga aggcgacctt ggtgagcacg gtggcgcggg
     6841 cgcggtcggc ggtcggaacg atcttcttga cgcgggcggg gtagcagacg gcggggtagg
     6901 cgtcgaggac gatctcggcc gggcgcccga cgacgatttt ttgaatgttc gactcggaga
     6961 cgtcagcctc cacctcaagg gagttcatgt cggcgagcgt gacgaccgag cctttggagg
     7021 aagcggagga ggcgaagggg gcgacgattt cgccgacatc ggcgttcttg gtcagaatgg
     7081 tgccgtcgaa gggagcgtgg atgtaggtgt tttccagagc cacctcggcg gcggcgaggg
     7141 ccgcgcgggc cgcggcgagg cgcgccaggg cggtcaggta ggccgattcg gccccctcgg
     7201 cgtcgatttg ggtgacggct ccggaggcga agagttcccg gacccggcga tgctggcggc
     7261 cggcggtgag ggtatcgacc tcggcggctt gcagttcggc gcgcgcctgt tcccgctggg
     7321 cgcggatgtc ggcgttgtcg agttccgcga tcacctgccc gacgcgcaca cggtcgccct
     7381 cttcgaagtc gaggcgggca agccgcccgg tgcccttgga ggcgacttcg gcgctccgct
     7441 gggccaccac gtagccggtc gcgaccaggt cggcggaggc ttccgatccg gtgagcaggg
     7501 tggcggcggc agtccggact ttcagagtcg gttggataaa ctcgcgcagg gcgaagtagc
     7561 cggcgacgag tgcggccagg atcgcggccc aggcgatcca gcgcccgacg ctccggcggg
     7621 ggcggtcgcg gtacttgcgg ctgcggtcga tgcgcaggga gccgatgtcg ggccggccgt
     7681 cggattcgct catgtgttct cgcttcggtc gccgcggttg aaagcacgga tgcaaacgca
     7741 ccagtgtatt caccggccgg gcggtgcgca agcgattagt tggcgcccgg ggcggggtcg
     7801 ggggggagcg gccgggcgac gcgcccaaag agcgcgccgc ccctcaccag atgccgagga
     7861 cgtccttggc gtaatcggag gccatcgtct ccgggtccca cacgggatcc cagacgagga
     7921 ggacttcgac ctcgcgcacg ccctccaggg cggcgacctc ccggtgaacc tgggccacga
     7981 ggatttcgcc gtaggggcag gccggagtcg tgagggtcat cttgacctcg actttgccgc
     8041 cgtcgtgaac gaccacgtcg tagacgaggc cgaggtcgac gataccgagg cggatctccg
     8101 gatcgtagat cggccggagc acttccatga tttgttcttt ggtcggagcg ggcatcactc
     8161 ggactcctcc gtcgagacgc tcggcgggtc ggccgggcgg ccctgttcat actgcctcag
     8221 gccctcgatg agagtgaccc aggccaggag cgcgcacttg acgcggaccg ggaagttgca
     8281 gacgccccgg agggcctcga cgtcgccgag atcgtcgggg atttccgtct cctccccctt
     8341 gagcattttg cgcacgatgc cggcgatacg cttggcctcc tcgagcgacc ggcccttgat
     8401 gacctcggcg agaatggagc cggaggcggt ggagatggcg cagcccttgc agtcgacgtg
     8461 gacgctcttc acggtgtcgt cggccatctc cagggcgagc gacagttcgt cgccgcaggc
     8521 cgggttgtgg ccgtgggagg cgacgtccgg ctgggtgatc ggtttctttc cgcggggaga
     8581 gcggaagtgg tcgaggatca cctcccgata catgtcatcg agattggtca taacccgaaa
     8641 tacctccgca tctcaaggag ggcgtcgacg agggcatcga cgtcggcttc gtcgttgtag
     8701 atgtaaaagg acgcgcgggc ggtggcgatt ttgccgagga ggcgcatgag gggctgggcg
     8761 cagtggtggc cggcgcggat ggcgatgccg cgggagtcga ggaaggtgct gatgtcgtgg
     8821 ggatggaggt cggggtcggt gaacgagatg gcgccgccgc gccgggcggg atcggtcggc
     8881 ccctggattt ccaggccggc cagcgtctgc agccggtcaa gggcgtagcg ggtcaggtcc
     8941 tcctcgtggc ggcgcacgcg gtccatgccg agggcctcga ggtagctcag cgcggcgtcg
     9001 aaagcgatga cgccggcgat gttgggcgtg ccgccctcga acttgttcgg cagatcggcc
     9061 caggtgacgc ggtcgagact gacctcgcgg atcatctcgc ccccggtgat gaagggcggc
     9121 atggcttcca gcagcgcgcg ccgtccccac aggacgccga cgccggtggg gccgagcatt
     9181 ttgtgggcgg agaaggcata gaagtccacc ccgagctcgc cgatgcggac ggggaggtgc
     9241 ggagcggcct gggcgccgtc ggccagcacc accgcgccgt aacgcttggc ggcggcggcg
     9301 atcgcgctca ccgggttgat cgtgcccagc acattgctca tgtggcagac cgccgtcagc
     9361 cgggtgcgcg gggtgatgag ccggtcgagc gacgccaggt cgagcgtacc gttgggctga
     9421 atcggaatct gcctgaggac agcgcgtttc cggcgcgcga gctccaccca ggggacgagg
     9481 ttggcgtggt gctccatctg ggtgatgacg atttcatctc cctcgcggag gttctcctcc
     9541 ccccacgtgt aggcgacgag attgatcgat tcggtggtcc cgcgggtgaa aatgatctcc
     9601 tgcgggtcga cgccgccgat gaagcgcgcg gtgtgctggc gggtgcgctc gtacagctcg
     9661 gtcgaccgct cggagaggtg gtgcaggccg cgatggatgt tggcgttgga ggtacggtag
     9721 tagtcgcttt cggcctcgat caccgcattc ggtttctggg tggtggcggc gttgtcgaga
     9781 tagacgaggc ggcggccgcg caccgttgtg tgcaggatgg gaaagtcggc ccgcaggcgc
     9841 tcgatatcga gcgccgtcag cggcgcggca gagggggcgg cggcatgtgg tttcatcggt
     9901 tgacctccca tcacggccgt tcactcgacg gcgacgtaga tgtcctcacc ttccactttg
     9961 acttcatagg tgtccagcgg caggatcgcg ggcggggcct cgacggcgcc ggtggcgacg
    10021 ttgaagcgtg cgccgtggcg cgggcagacg acctgctcct tgaagaggcg gccggtggag
    10081 atcggggcgc cgtcgtgcgt gcattcgttg gctacggcat agatacgccc gcccacgttg
    10141 cagaccagga cgtcggcgta gcccacccgg tacgccttca tcgtgccggg cgggatgtcg
    10201 gcgacggccg cgattttgtg atacggcgcc atgatctata ggccctccag acggcgctcg
    10261 atgaactccg ccaggcggcc gcgcaggtca tcggggatct gccggagcgt cggttcgacg
    10321 aaggcggaga cgatcatgcg caccgcgacg tcgcgcggga tcccgcgcga ggcgaggtag
    10381 aacaccgact cggggtcgat cgggccgatg gtggcgccgt gagtgcactt gacgtcctcg
    10441 ttgagaatct ccagttcggg aatggtctcg gctttcggcc cccggttgag cagcaggttg
    10501 cggttctcct ggtaggcctc gcaggttttg gcgtgctgct cgatgcggat gagcccggtg
    10561 taggcggaga cggcccggtc gcggagcacg gttttgaaat cgatggtcga agtggagttg
    10621 ccggcgccgt gggcgtggag cgtgtggttg tcaaagtgct gcccgcccga gccgaaaagc
    10681 atgccgcgga ggcggctgtc ggctccccgg ccggccagca gaacgccgaa gttctgtttc
    10741 gtgatgttgc cgccaatggc gagggggacg gtgaggacgc gggcgtcccg cccgatctcg
    10801 gcccggtgcg tcaggtacat gttcacgccc gccccgtggc gctgcacgct cacatagcgg
    10861 acgcggctgt cgtcgccggc ggagatctcc acggcgccgt tgacgtgggt cacgcccccg
    10921 gaaacgtcct ccgagccgcc ggcgtactcg tcgatcagag ttatctcggc gttgcggccg
    10981 acaatgacga gcaggcgcgg aaagtagacg gaggagcggt cgccgccctc gcggaagagg
    11041 tggatgggcc gatcgatcgt ctcgccctcg gggacataga cgaagatgcc gtcattccag
    11101 agggcggcgt tgagggcctc gaacttgccc aggtcgggac cgacgagacg gtagaggtgc
    11161 tgttcgacca ggtcgccgtg ctggtcgacg gcgagggaga ggggttggat gacgacgccg
    11221 cggcgggcga gccggtcggc gccgtacgtg gatatttcgc ggccggcgta gtcgacggcg
    11281 agtgcggcga gcgcgccgct gtcgacgtgc tgcttctcca gtccgatgat ggcgtcgcgg
    11341 tcctcgaccg gcgggtggtc ggcggccgac ccgtgcgggg cgagaaaggc ggcggggtcg
    11401 gtgtaccgcc agaggtgcag accgcggcgc gggaacggcg cggcgttgaa aatctgggcc
    11461 gcgctccggc gggcgtcgcg cagccaccgg ggcccgcggg ccaggccggg ggcgaaatcc
    11521 gggatggcgt gctcggcgcc ggcagtgtca agagtgggtg tcgtgctcat ggctatccaa
    11581 tcgagccttc catctcgagt tcaatcaggc ggttcagctc caccgcgtac tccatcggca
    11641 gctccttggt gaacggttcc atgaagccat tgacgatcag cagtcgggcc tcgtcctccg
    11701 tcagaccccg gctggtgagg tagaagagct gctcctcggc gaccttggag acgcgggctt
    11761 cgtgttcgac gcgcacctgg tcgtggtcga tttccatggt cgggtaggtg tccgagcggg
    11821 cctgcgcgcc gatgaggagg gcgtcgcact cgaccgacac cttggcgtgg gcggcgttct
    11881 tgtgaatctt gaccaggccg cggtaggaag cccggccgtt gtctttcgag accgacttcg
    11941 aggtgatgcg cgacgaggta tggggcgccg cgtggatcac tttcgccccg gcgtcctggt
    12001 gctggtcgat gccggcgaaa gcaaccgaga ggatttctcc ccgcgcgcct tcgcccacga
    12061 gctggatgca cgggtacttc atggtcaggc gcgagccgat gttgccgtcg acccactcga
    12121 ccgtggcacg ggcgtgggcg gtcgcccgtt tggtcacgag attgtagatg ttgcgggccc
    12181 agttctggat ggtcgtatag cggatgtagg cgccctcgag ggcgatcagt tcgacgaccg
    12241 cggagtggag cgagtcggtc gagtagacgg gcgcggtgca gccctcgatg tagtggacgc
    12301 gcgagccttt gtcggcgatg atgagggtgc gctcgaactg gcccatgttc tcggcgttga
    12361 tgcggaagta ggcctggagg gggattttca cgtcgacgcc cggcggcacg tagatgaacg
    12421 agccgcccga ccagacggcg gtgttgaggg cggcgaactt gttgtcgtgg gaggggatga
    12481 cggagccgaa atactgccgc acaacggcct cgtgctcgcg caggcccgag tccatgtcga
    12541 ggaagatgat gccctggtcg atcaggtcct tcttcatcga gtggtagacg acctccgatt
    12601 cgtattgggc cgagacgccg ccgaggaact tcttctcggc ttcgggaatg ccgaggcggt
    12661 cgaaggtttt cttgatgtat tcggggacgt cgtcccaggt ctcgcccttc ttctcgatcg
    12721 gcttcatgaa gtagaagatg ttggcgaagt cgatttcgtt gagcagggcg gtgttccccc
    12781 accggggcat tggcttggcc aggaaaatgt cgagcgcctc gtggcggcgg tcggccatcc
    12841 agcggggttc ccccttcatg cgggagatca tctcgacgac ctcatggttg atgccgcggg
    12901 cccctttgtg gaaatagtcg accgggtcgc ggaagccgta cttggcggcg tagtcctcgt
    12961 tgagcgcggc caggccggcg ttctgattct gttttgccgt ttccgccatg ggtcacacct
    13021 ccgcgacagc ggccgacggg ccgaccaacc agtcgtagcc tttggcctcg agctccaggg
    13081 cgaggtcggg accgccggat ttgacgagcc ggccgcgcga catgacgtgc acgtagtccg
    13141 gcttgacgta gttcaggagg cgctggtagt gggtcaccag gagcacgccg tttctctcgg
    13201 tgtggaaacg gttgatgcct tcggccacca tcttgagagc gtcgatgtcg agaccggagt
    13261 cggtctcgtc cagcagcgcc atcgcgggct tgagcatggt catctggagg atctcgatgc
    13321 gcttcttctc gcccccggag aaaccgtcgt tgaggtagcg ggcggcaaac tggcggtcga
    13381 tgccgagctc ggccatgtgg gcgaggaaaa ctttccggaa gtcggacatg tcggctccct
    13441 tgccccggtg ggcctgcacg gcggcgcgca tgaagttggc gaccgtgacg ccggggatgg
    13501 ccagggggta ctggaaggcg aggaagagac cggcgcgcga acgctcgtcg gcttccatct
    13561 ccaggaggtt ccgcccctcg agccaggctt cgcccgcggt gatgcggtac gacgggtggc
    13621 ccatcagggc cagggagagc gacgatttcc ccgaaccgtt ggggcccatg atggcgtgga
    13681 cttcgccggg gcggacggtc agggagacgc cgcggacaac ctccttgcca tcgacttcga
    13741 cgtggacatc tttgaaagag aacagcggtg tatggtcaga catgatgggt cttccttcat
    13801 cctagagtga ttgttgactc tgtgtgtcac gggccagccc gtcgatctcg gcggggcggg
    13861 gggtgatatc ggtagtggcg gccgcgccgc cgagcagttg ctcggccccg atgagccccg
    13921 gtcccgccgg acgcccggcg ctgcgcgcca ccagatcctg cagcgtcgtc cgcccgagaa
    13981 tgtccgcgat gtaggcggcg agcgtgtcga gcacgtggtg gacgctgcag tcgtcctgat
    14041 gcacgcacac ctcccgccgg ccgctgaagc gggcgcagtg gtcggggtcg atgatcgggc
    14101 cgcccagggc ggtcatgacc tgaaggaggg tgatgcgttc cggctcccgg ccgagacaga
    14161 agccgccgct gcggccgcgc acggcggtca ccagtccggc tttgcgcagt atcgccaaaa
    14221 gcttggaggc atacgggacc gagaggcctt ccaggtcggc gatctcgctg atcgacaact
    14281 ggccatccgc ccctcggcgg gccaacgcca gcaggcagcg cagtccatac tcttcgagcg
    14341 aggatattct catcaacagg cctctcggaa tctgattgac accgggcaat atttagctct
    14401 tggatccatt atacaaaaag tttacgcaaa tgtaaacatt ttacgtccaa taactgcaaa
    14461 tttctcggcg ggttcccttg atggaggtca agaccggtga aaaaaagcaa gaaattccgg
    14521 tttcacaaaa gcttgcggcg gacaacggaa tacgctacct ataggggtcg gagaacccga
    14581 tggaaaggta gacaagacat cgacttaatc aacgtcgtcg cccttgcgtt cgccctggcg
    14641 ctcgatgctt ttggcgtggc tatcgcggtg ggagtcacag ccggagcgat tgaccggtgg
    14701 acgattttcc ggctctcctg gcatttcggg ttcatgcagt tcgggatgcc gatagtcggt
    14761 tggatggcag gaagttacct cacgggttgg gtggggagtt tcggccgctg gctggccgcg
    14821 gctgtgctgt ttgcaatcgg agcccgcctg atctgggagc aggcgaatcc ggaagtccgc
    14881 aagtggaagg gggatccaac ccgcggcgcc agcctgctcg tattaatggt cgccacgtcg
    14941 attgatgccc tggcggccgg gttatcgctg gcgctggtcg gggtggcgat cttgtatcca
    15001 tcgatggtga tcggggtggt cgccgccggg atgaccgttc tcgggctggc tttcggtcac
    15061 gccgtcggga tgaagctggg gcggatcgcc ggcgtgatcg gcggcgtcat tctgatcgcg
    15121 ctggcggttc gggcggtggt gggatagggc ggaacgccgg tgcgccggtg gcccctcact
    15181 gccggaccag gcggaggcgg tacccggctg cggcgcccgc ggcggacggc tgggagagaa
    15241 agagcgtgtc gccggtcacg cgccaggtga atgcgccgcg gagcacgatc gcggggcgga
    15301 ggattttcgg gtaggctccc tcgagtgtga cggtggaatc ggtccaccgg tacggtccgc
    15361 cgccgtcgcc ggcctcatcc tcgggaatcg tgtccccctc gccatagaca tacgccttgg
    15421 cgtcgaagat gaaccagacg tcgacagttt cagcgtaagc ggagtcggcc gggtcggccg
    15481 gcgtgaaagc ggaggcgccg acgtatcgtc ccacgaaggg ggccgccttg ggcggttccg
    15541 atttctccac gggcatcaac aagaataccg cagtcacaac ggcataagca agcacagtca
    15601 tacaggctcc cgcgcttcgg gttgcggatg cgccgggccg ggccgcgcgc ggtcggggaa
    15661 ggtgggcgaa aagcgcttgc ggctcccggt cccagcagtt atgatgtgaa agatttcaca
    15721 ggtgcgcaag acgatactta cgcggcgccg gcccgcggcc cggggcggcg gcgatgaaga
    15781 cagatgaatc taccacgatg agcggtttta tctatgagag aattactatc cggcaatgaa
    15841 gcggtagccc ggggggcgtt cgaggcggga gtccggctgg cggccgccta tcccggcacc
    15901 ccctcgaccg agattctgga aacgctcgcc gagcggtatc cctcgatcta ctcccagtgg
    15961 tcgccgaacg agaaggtggc ctacgaggtg gggatcgggg cctcgctggg cggcgcgcgc
    16021 acgctggtgg cgatgaagca cgtcgggctg aacgtggccg ccgacccgtt catgacccac
    16081 gcctacaccg gggtcaacgc cgggttcgtg atcgtgtcgg ccgatgatcc cgagatgcac
    16141 tcgtcgcaga acgagcagga caaccggcac ttcgccaagt tcgcccaggt gcccatgctg
    16201 gaaccgtccg attcgcagga agccaaggac atgacggtaa cggcgctgga gttgtcggaa
    16261 gtcttcgaca cgccggtgct cctgcgggtg accacccgga tttcccacag caaggggatc
    16321 gtcgagctga gcgaggcggt caagggaccg gacaagaaat tcgtcaagga accctccaag
    16381 tacgtcatga tcccggccaa cgcgaagaaa cggcacgtgg cggtggccga gcggctggcc
    16441 cggctccgga ggttctcgga agagacgccc atgaacacgg tcgaggagaa cggatcgcgg
    16501 gtggggatca tcaccggcgg ggtctcctac cagtacgcca aggaggtcat gccggaggcc
    16561 gactacttca agctggggat gagttacccc ctgccgctgg agaagatcgc cgcgttcgtg
    16621 cgcgcccacg agcaggcgat cgtgatcgag gaactcgagc cgttctacga agagcagatt
    16681 cgggcggccg ggatcgcggt gaaggggaag gagtatttcg gggtgcaggg ggagctctcg
    16741 ccgcaccggg tggcggaggg gctgttcaag gcgggggtga tcgaacagcc gcgggcggcg
    16801 gagatcccgc cggcagccga catgtttccg cggccgccgg tgctctgccc cggctgtccg
    16861 caccgcgggg cgttcatggc cctgcgcaaa ctcggagtcg cggtcaccgg ggacatcggc
    16921 tgctacaccc tcggggcgct ccagccgctc aaggcgctcg acacctgcat ctgcatgggc
    16981 gcctcgatcg gcaatgcgat cgggatggag aaggtgcagg gattcggcaa gggaacggtc
    17041 gcggtcatcg gagattccac cttcctccat tcgggcgtga ccgggctgat ggatgcggtc
    17101 tacaaccaca gcaacgtgac ggtgatcatt ctggacaacc gggcgacggc gatgacggga
    17161 gggcagatgc acccggggac gggccgcacg ctcatgggcg agccggccca cgaaatcaac
    17221 atcggccaac tggtgagagc cctcggggtc aagaactacc gcgaggccga cgcctacgac
    17281 tatgagcaga tgcaggaggt gatccgcgag gagattgaca agccggggcc gtcggtgatc
    17341 cacaccacgc ggccgtgcgt gctcatgccc aagcgaatca tggacgaacc ctacctggtc
    17401 gatctcgacc tgtgcaacgg gtgcggggtc tgtttcaaac tctcgtgccc ggcgatcgcg
    17461 gccgccgcgc agctcaacga gaggggcagg cccaaagcga cgatcgacat cacgctctgc
    17521 accgggtgca cgctctgcgc gcaggtctgc ccgacggagg ccatccaact gcagagccag
    17581 tttgcgaagg agggtgcgtg accatggcgg acgaccggtt caacatactc attgtcgggg
    17641 tgggcggcca gggcgtgctc ctggcctccg agatcatctc ggaggtggcc atgcaggcgg
    17701 gattcgacgt caagaagtcg gaggtgcacg gcatgtcgca gcggggcggg gtagtcagct
    17761 cgcacgtgaa gatcgcgccc aaagtctact cgccgacaat cggctacggc caggcggacg
    17821 tcgtcatggc gttcgagcag gccgaagggc tccgcgcggt cgactggatg aagggggacg
    17881 gcgtggcgat cgtctcggag accacgctca tcccggcgat cgtgacctcg tcgaaggaat
    17941 tcacgtaccc gaaagacgcg atcggcgcga tgcgcaaggt cgcccggcgc gtcatcggcg
    18001 tcccggccga caagatcgcc ggcgaactcg gcaacccgcg gctggtgaac accattctgc
    18061 tgggcgtgct gtcgaattac ctccccttcg atctcgacct ctggaaagag gcgatccggg
    18121 cgcgggtgaa ggagaagttc gtgccggtca acctcgcggc gttcgaccgg ggccgggcgg
    18181 tgaagctcgc gcccgtccac gcctaggcgg cgggcaggcc gatggctgag aaactgctga
    18241 taatcaaccc ggggtcgacc tcgacgaagg tggcgctctt tgaaggggac cggcggctgg
    18301 ccgaggagac ggtccgccac gacccggccc ggctgcgcgc gttcgacaac gtggccgagc
    18361 agttcgcttt ccgcatggag acgatcgatc cgtggatcgc cgccctcggc ctcgcggcgg
    18421 gggagatcgc ggcggtggtc ggacgcggcg caccgctgcg gccgctcgag ggaggctcct
    18481 accggatcac gccgcagatg ctcgacgatg ttcggaccat gcgttactcc aaccacgcct
    18541 ccaatctcgg ctgcctcatc gcccaccacc tggggctccg ctacggcgtg ccggcgttca
    18601 tcgccgatcc gatcacggtt gacaatttca ccgacattgc gcgcgtctcg ggcgtgcccg
    18661 agatcgagcg caagtgccgg gtgcacgcgc tcaacatcaa ggaagtcagc cggcgggagg
    18721 cggagaagat gggcatgacg ctggcgacgg ccgctttcgt ggcctgccac atggggggcg
    18781 gggtctcggt cgccgcgctc cgcggcggcc gggtgatcga cgtcaacgac gcccttctcg
    18841 gcatggggcc gttctccccc gaccgggccg gcgccctccc gatcggcgaa ctggtccggc
    18901 tgtgctacgc ggggaagtac accgagaagg agatgatcga caagctgtcg aagcactccg
    18961 gtctggccgc c
//
LOCUS       DAOPOE010000062        18925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3539_length_18925_cov_5.304028, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000062 DAOPOE010000000
VERSION     DAOPOE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18925
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3539_length_18925_cov_5.304028"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..4680
                     /locus_tag="PKM94_09145"
     CDS             304..4680
                     /locus_tag="PKM94_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66843.1"
                     /translation="MTCRASASTVLVAVVLLAISVVARSPLPPVESDPGRPALPPLAL
                     SPDSVCDPGVVWPTAYGVQQTGEINAAFTAAGKFGLRFFYGPWVDSWPSQGFETPAYS
                     GVDYLFTGGLWVGGVVGNDTLVSVTYDGWVNVEEFFPPRLVPEVDRFPAVSDFGLRTS
                     FTDTCASCPSEDPIDSTHRPLDVKVALRSHAWRTPPLDKHIFYDAVITNIGSELIEAG
                     YVGLYVDGDVVWVGSTGEGWTDDLAGAVREVGTAYIIDNNGDFGSGEPEKLRALAARF
                     LNASQSPSDTNCNWWISNGYAELDFGPRLRGAPDDPFRDFGTGGLGTPTGDANKYYVL
                     RHREWDYDQFATAAIDSADPLWLTPPAALAVDLCNGYDARFLLSLGPFTLAPDSSFRV
                     IFSMFTADSIHTVPGNLAHLPADPAAYRDNLGLERLAAGAAAGETALPLLLDPLQAPA
                     WLEVAPGYGHSDLSLEWDPWVYDEVTGYNIYVTPLDPGLLPHPGAVPPWLIPESPVLA
                     GTAGRTYKAVLPCDPGVFAAVNIAHVSAAGAGALSTSEFIHVPSPSAPDVRSMWFVPG
                     EEEAVVHWPAQAGVDRYHVYRGTRYFAPFYSEGRLAASSYGEPFDSVTADGRWYYYYR
                     MAPYLEVPALESSFTETVADSADFLITAVNAYGFESAPRPVKVLRVPVRDKSLMVLTK
                     TASGPGGIFSPTAFCEQFYQAAFRYEIFDYINAADPQWLQLMPYDMVIIDGPFHDGFP
                     SPTGGFPVALERYLKSGGKLVCFSNLGAYYGMGYLTGPGTRALPSSVRSWFGVDSATC
                     TGYGYYYTNGIPHVDSIFAFHTAEPIGGGFPSLSCDTAAFPLAANWTTFWPETSPPSV
                     ATFTLSSDPSADAVATHVFRSLAPATSLQEGGIVGVKSRVAFFDDTSDVHLFGFHLYH
                     MDTLDARALLAALRTPNSAPALALTGEPGYERKGVVPQWGNPGTAFEFRVEFSDPDGD
                     LPLPGYPRVHLYSALTGGPAVGSPFPMTAVSGARSAGQVYAAVVQDLPPDPSYTYWFE
                     ARDEHGAAAAGAVTAAHRFYGPVVTDPGGAVRWVAVTGNDTTGTGDEGAPLASVQRAA
                     DLANNGDTIRIGDGVYPGAFLSFWGKQLVIESVHGAAATVIDGGLSPNVIASTKIGVP
                     LAGFEDSTFVLRDLTLRNFGNDTLDGRVLDLNGSSPSIRDCRFEATRGRSAGAVVCRG
                     GSPVFEGCAFIANRADSAGGAVWLDSCEIRFVGCTFAGNAAPLGSAVWASASHVTLER
                     CILAYGQGSPPVAFDPAHPGAVTVSCTDLFGNAGGDWSGCLAGLGTGDNLTRNPQFCD
                     TTGGDYHLFVHSPCRAEMNNCAALIGAYAVDEACAGCCALRGDLTGEGSVTVADITAL
                     IRQVFRGGAEPGCPEHSNVNGGLGVSLIGPVSVVDITYLVNYVFRGGSAPPACGFPGA
                     PEEVVPDFGAVGGAAPVPSPRP"
     gene            complement(4768..6285)
                     /locus_tag="PKM94_09150"
     CDS             complement(4768..6285)
                     /locus_tag="PKM94_09150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD66844.1"
                     /translation="MPHKTTVAALVLGGLALLVLGCSGGSDDSPRLTLPTAYDQALIT
                     QYIQLSNEGYGALERGQQDSALAAFRKQAEVIPEGRWGLYNLACAYSRMGNADAALAY
                     LDTAVTRGWTVVDHLENDPDLAPLRDDPRFAAILDKARQLDTEKMGMLAAGLPRDVTP
                     PQGITDSAALVAWFDGQQEILRVNSQVWHPWQTAAANLDLEAKRLAATEALLGDRFDY
                     DLERIISLGRMNSPYDEKWGAVSQTIMKEVDAYLATSPNAESASEACYRGVAAAIMEH
                     GPAATGTPEGQAALAKAESYYGRMDSSYRRYGGAEGWILAARLGAAGEARETLYPQIK
                     DFAARNAADENAMAVAKTLFPADMVKASWPIPLTATDIDGKPVSLEDYRGKVLLLDFW
                     ATWCGPCRAELPYLKAAYEKYRSQGFDILSISLDYPNQTTQDAYRAWITEAGMPWRHV
                     YDEQNWTGGITQAFAVGSIPSPFLIGKDGTLIAMHDTCRGPALDTLIQKALAMQM"
     gene            complement(6392..7570)
                     /locus_tag="PKM94_09155"
     CDS             complement(6392..7570)
                     /locus_tag="PKM94_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOD66845.1"
                     /translation="MAHDRSKNWQFETTAIHSGRVPEDGTRSVTTPIYPSSTYRVDYP
                     GDESGYVYARWKNPTRLALEETLAKLERGTKAAAFASGLAALDAVLKLLKTGDHVVAV
                     DDLYGGTMRQFERIGRRFGLDFTYVDGRDPKNLESAIRPNTRLFWLETPTNPLMHLVD
                     IEKVAAVAKERGILVGVDNTFATPYLQQPLELGADIVLHSMTKYLGGHCDLVAGALIV
                     KDSSLGEQLWFNQYAGGAHLGPWESWLVLRGLKTLALRMERHSVNAMKIAEYLETVEA
                     VEKVYFPGLDGRPVPNRMRLPGGMVAFTIAPQFDFEAVKRFVMALRVFVLAESLGGVE
                     SLVNHPAAMTHSSIPREIREPRGITDGLVRLSVGIEHIDDLLIDLRSAFDALTQSARA
                     "
     gene            complement(7579..8610)
                     /locus_tag="PKM94_09160"
     CDS             complement(7579..8610)
                     /locus_tag="PKM94_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOD66846.1"
                     /translation="MNSHNPTPLPARLRDIAYAEDMTETAGNTPLVRLRVLPREWGIK
                     ATILVKPEFLNPTGSVKDRMAIYLLRQAERKGELSPGGTIVEATSGNTGAAVAMFAAA
                     HGYRAILTIPDKMSAEKINALRAFGAQVHVCPTAVAPEDPRSYYETAKRIARETPHSY
                     FVGQYFNTDNIEAHYRTTGPEIWRQTKGKIDVLVGGIGTGGTVSGTARYLKEQNPKVA
                     VIAADPEGSVFYHYFKTGALPEAHPYLVEGIGDDFLCPTLDLTVIDDICQVSDRDCFL
                     MARDLTRKEGICGGGSSGGIVLAALRYAQEADLAEDKLLVAILPDHGNKYISKIFNDE
                     WMREKGFIE"
     gene            9228..9452
                     /locus_tag="PKM94_09165"
     CDS             9228..9452
                     /locus_tag="PKM94_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66847.1"
                     /translation="MRCAECGDKITGKPIKRGNRVFCSIDCAESAAQDRYDDEEDQEL
                     DDEFDTLDEEEVDFEEDSEDRFLGGEDDER"
     gene            9858..10457
                     /locus_tag="PKM94_09170"
     CDS             9858..10457
                     /locus_tag="PKM94_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66848.1"
                     /translation="MFKRVSLALVALAMLLAAAGCSKAPEAEMQASEAAMQAAVAAEA
                     EQYAPEAFQAALDTLNSAKALKAEQDGKFSLFRRYGKAAEMFVRAQTLSEQAATAAAE
                     EKERVRVLVAGLLTQADSVLTQATQLVDKAPRGKGNKAEIELMRGELSSATTALADAR
                     ADFDRGQFLVAKTKVEAVSQRLNSLINEVNTAFSRTGQR"
     gene            10562..11632
                     /locus_tag="PKM94_09175"
     CDS             10562..11632
                     /locus_tag="PKM94_09175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOD66849.1"
                     /translation="MRRTLRRLTFAAVLIGGLGGCEDAPEGLYRQADSAVRRVRTQTT
                     GAYADSLLRQADSLLLSGRLEMARQNGRIGPFRDFAAAESLLTRALQTAVAASDSAKV
                     RHDRARDAALARRDELLTELKTWREALNGFLRLHGAEKRLTNAELGLQIGRRLIDSGK
                     YAEAMEVMDDVRDSLGRIRDIVDEYMRDESRHISTWRRWVNETIQESRATGTSALIVD
                     KAAHRAYLVSSGRLVKSWPADLGHNPSRQKLFAGDGATPEGMYRVTEVRRRGSKYYRA
                     LMLNYPNELDRRRFMENKKAGVISSGAKIGGLIEVHGEGGQNRDWTDGCVALSNEDID
                     ELFQHVQKGMPVTIVRRSDKWP"
     gene            11629..12264
                     /locus_tag="PKM94_09180"
     CDS             11629..12264
                     /locus_tag="PKM94_09180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOD66850.1"
                     /translation="MRYVVIVLIVALTVTFAAIYLSPPDEADGSRAAQVVGDTAARLP
                     EGRSLRSQLALLQRDLKRVAPREPYIVINTHASKLSLRTADSVLFEAVCSAGSGAELT
                     DSTTGRRWVFDTPRGMYKVTSKIKDPWWRKPDWAFVEENEPVPEDENERLDPRMMGEY
                     AIGFGDGYFIHGTIYERLLGVNVTHGCVRLGAEDLRYLYKKVPIGTRIYVF"
     gene            12254..12958
                     /locus_tag="PKM94_09185"
     CDS             12254..12958
                     /locus_tag="PKM94_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66851.1"
                     /translation="MSFSRPRLRALIALGVLAAAAIASCEQQQAASQTTGAADPGEAR
                     LEREYMRALAEMELARGDRTYLVIDPGRRALLLKLKGAVVFSVPITVVDDDTALSAGE
                     VTDRLREFGLDLLGDDVEVSFLRDRYMFSRAGVIPDSILQIICDAVNADPALVQRFLP
                     ERFVLEWEGGAVLDVRTTVKGQATSAFGNIMAGLKQIIKRPFTTQLRVQMDAEAALTL
                     YSVCEPGTPTILTAMQ"
     gene            complement(13064..14386)
                     /locus_tag="PKM94_09190"
     CDS             complement(13064..14386)
                     /locus_tag="PKM94_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOD66852.1"
                     /translation="MRFLNIVILLWAVAASGAAAQTAASADACEDCHQKQTAGLHRQW
                     QESLHGQYGVGCRDCHAAAEGDPDAFAHNGALIATLVTPADCRACHEKEATEVERSYH
                     ATAGQILESNDAYLAHVAGGAPAAIAGCENCHGAKVQIDPNRPNRLARQSWPNAGIGR
                     INPDGSKGSCTACHTRHTFSAAQARRPEACGKCHLGPDHPQREIYDESKHGNAFYTNV
                     DKMKLAGDSWVVGRDYAAAPTCATCHIGATPASPGTHDVGERISWTLRPAVSVHKENW
                     QEKRAAMKRVCANCHGGGFVDGHYYQYDAVVALYNEKFARPAGEILARVRAQGLLERP
                     ASFGNEIEWTYWEIWHHEGRRARHGAAMMGPDYTWWHGLYEVAKHFYFGLLPEARAYG
                     NAAVNAYIDSLLANDPMHAWVNRETDQLTREIRSGKMQEIYEAMFPEQ"
     gene            14616..15908
                     /locus_tag="PKM94_09195"
     CDS             14616..15908
                     /locus_tag="PKM94_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOD66853.1"
                     /translation="MRTLVQNWLINHRVNVAAAEWLSWLIVGVAVAGAMVIAKTAAQV
                     IVLRIVRYFVMRTQTTWDDALLHRRFFNRLAHLAPGLVLYFCAGLFPSIREGIERAAM
                     VYMIMTGLFAFSAFLSAVDDVYQTFSISRQRPIRGYIQVARIILFVFVGIVAIATLMN
                     RSPWLLLSGLGAMTAVLLLIFRDSILGLVASVQLSSNDMVRIGDWIEMPKYGADGEVV
                     DVTLHVVKVRNWDNTITNIPSYHLIADSFKNWRGMQESGGRRIMRAVCIDVSSIRFCT
                     PEMLDRFEQFQLITEYVRQRRGEIEEWNRAHGIDRRHPVNGRNMTNLGTFRAYVSAYL
                     RRHPLINPDLPLLVRHREPTEKGLPLQIYAFSRDKIWTNYEALQADLFDHILAAVPLF
                     DLRIFQLPGGHDVRGLAALLPQDHRPAPPAAAGGAPSP"
     gene            15960..17072
                     /locus_tag="PKM94_09200"
     CDS             15960..17072
                     /locus_tag="PKM94_09200"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOD66854.1"
                     /translation="MPRITRPEPERDLFKNRYSRASVTAMGRAIKAVCPTFDLPGFVA
                     SVITDDFPRLEFKDRGRAIARALAEYLPGDYGRAVDVLIAVAPRLDGFHNWAAMTFIE
                     LQGLAHFEDSVRGMKALTRYSTAESAIRPFLNSFPDRLLPILHEWAADPDEHVRRLAA
                     EGSRPRGVWVAHIGAYKKDPRPVLALLEKLKADPSKYVRTAVANNLNDISKDHPDLVI
                     TAALRWLKDGDAGTTWIVRHACRSLIKKGDPRVFPIFGFTANPKISVTGPRPPRRAVA
                     IGADAAFPFTVTSRAQSRQKLAIDYRVHYVKKNGKTSPKVFKLAEKTIAPGETLALQV
                     RQSFADVSVRKHVPGRHRLEIIVNGRTVAAADFTLA"
     gene            17355..17939
                     /locus_tag="PKM94_09205"
     CDS             17355..17939
                     /locus_tag="PKM94_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD66855.1"
                     /translation="MDEKEQQLVRRAQAGDFDAFTELIAAHRTRLYNLALKMTGSQLD
                     AEDIVQDTFLKAIDRIDQFRGEAAFGTWLYAITLNETRMALAREKRADLRPIEDYLPA
                     HDRDHGGPTVYDWRDPHRLLEQGELRTAIDRAIGELPLPYREAFVLRYIDELPIKEVA
                     ELLGESVAATKSRVLRARLAVRDRLAKQFEGHNG"
     gene            17932..18180
                     /locus_tag="PKM94_09210"
     CDS             17932..18180
                     /locus_tag="PKM94_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOD66856.1"
                     /translation="MGKKCAEYVTDLNDYLDGEISPELCEELERHIGRCENCRIMVDT
                     LRKTVILCREGRQEKLPESLQQKLTDLLRQEWRKKFGK"
     gene            18341..18583
                     /locus_tag="PKM94_09215"
     CDS             18341..18583
                     /locus_tag="PKM94_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOD66857.1"
                     /translation="MTLENAVRLWAGFVVLLSLALYLWVSPWWLLLTAFAGFNMVQSS
                     FTGLCPAQWMLKKLFFKDQAEAAAPAGRRSAQSSRA"
     gene            18683..>18925
                     /locus_tag="PKM94_09220"
     CDS             18683..>18925
                     /locus_tag="PKM94_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66858.1"
                     /translation="MMKRHMRFLPIMASALGLALVWAFGCSSPTKDVFAPDEKTYQVA
                     GALIKEPNSNIASALMQVFKNGDTAETMTILVGDDTL"
BASE COUNT         3082 a         6374 c         6130 g         3339 t
ORIGIN      
        1 tcgcagtttt tgaaccccga gaagtcgcgc gactacctcg aggggattgc cgccaccagc
       61 cgctcgttcg ggctcctgac gcgtgtgaag tacggccagg ggttccatgc cgtcgagcca
      121 atcctggtca aggacatcat gacgctcgcg gagagctggg agggctaggc ggcgaggggc
      181 gcggcggccg cggaggggaa catggagaga ttggccttgc tttcggtgcg ccggtcgtgt
      241 ttcttggcgc aaaaccggtc caaccgctga atcggccgca gggcccccaa ggaggtctct
      301 cccatgacat gccgcgcatc cgcgtcgacg gtgctggttg ccgtcgtgct cctggcgata
      361 tcggtcgtcg cccgcagtcc gctgccgccc gtcgagtccg acccggggcg gcccgcactc
      421 ccgcccctgg cgctttctcc cgattccgtc tgcgatcccg gtgtggtctg gccgacggcc
      481 tacggcgtcc agcagacagg ggagatcaat gccgcgttca cggccgccgg taagttcggc
      541 cttaggttct tctatggtcc gtgggtggat tcatggccgt cccagggatt cgaaacgccc
      601 gcctacagcg gagtcgatta cctgttcacc gggggcctct gggttggcgg tgtcgtcggc
      661 aacgacaccc tcgtgtcggt tacatacgac ggttgggtaa acgtggagga gtttttcccc
      721 cctcggctcg tgccggaggt tgatcgtttt cccgccgtgt cggatttcgg cctgcggacg
      781 tcgttcaccg atacgtgcgc atcgtgcccg agcgaggatc cgatcgacag cactcaccgg
      841 ccgctggacg tgaaagtcgc gttgcgcagc cacgcctggc gcaccccgcc cctggacaaa
      901 cacatcttct atgacgcagt catcaccaac atcggcagcg aactgatcga ggcgggttat
      961 gtcgggctgt acgtggacgg cgacgtcgtt tgggtcggaa gcacgggtga ggggtggact
     1021 gacgatctcg ccggagcggt gcgggaggtc ggcacggcgt acatcatcga taacaacggg
     1081 gatttcgggt cgggtgagcc ggagaagttg cgggcgcttg cggcccgctt cctgaacgcc
     1141 tcccagtccc cgtccgacac aaactgcaac tggtggatct ccaacgggta tgccgaactc
     1201 gatttcgggc cccggctgcg cggcgccccg gatgaccctt tccgtgattt cggcaccggc
     1261 ggtctcggca cgccgaccgg cgacgccaac aagtattacg tgctgcgcca tcgggaatgg
     1321 gactacgacc agttcgccac ggcggcgatc gactcggccg atccgctgtg gctgacgccg
     1381 ccggccgcgc tggcagtcga tctgtgcaat ggctatgacg cgcgcttcct cctctccctc
     1441 ggcccgttca ccctcgctcc cgattccagc ttccgcgtga tcttctccat gttcacggcg
     1501 gactccatcc acactgtccc cgggaacctg gcccatcttc ccgctgaccc ggccgcgtac
     1561 cgtgacaacc tgggtctcga gcggctcgca gccggcgcgg ccgccggcga gacggcgctg
     1621 ccgctcctgc tggatccgct gcaggcgccc gcctggctgg aagtcgcccc ggggtacggg
     1681 cactccgacc tctcgttgga gtgggatccg tgggtgtacg acgaagtgac cgggtacaac
     1741 atctatgtca cgccgctcga tccggggctt ctcccgcacc cgggcgctgt gcccccctgg
     1801 ctcattcccg aatcgccggt tctcgccggc accgccggcc ggacgtacaa ggctgtcctg
     1861 ccgtgcgacc ccggcgtgtt cgccgccgtc aatatcgccc acgtctccgc cgccggcgcg
     1921 ggggccttga gcacgtcgga gttcatccac gtcccgagcc ccagtgcgcc ggatgtcagg
     1981 tccatgtggt ttgtgccggg ggaggaggag gcggttgttc actggcccgc ccaggcgggc
     2041 gtcgaccgct accatgtgta ccggggcacc cggtacttcg cgcccttcta cagcgaaggc
     2101 cggctggccg cttcgtccta cggcgagccg ttcgacagcg tgacggcgga cggcaggtgg
     2161 tactactact accgcatggc gccgtacttg gaggtgccgg cgctcgaatc gtcgtttaca
     2221 gaaaccgtcg ccgacagcgc cgatttcctc atcacggcgg tcaacgccta cgggttcgag
     2281 tcggccccgc ggccggtcaa ggtgctgcgg gtgccggtgc gggacaagag cctcatggtg
     2341 ctgacgaaga cggcgtcggg gccgggcgga atcttctccc cgaccgcctt ctgcgagcag
     2401 ttctatcagg cggcgttccg ctacgagatt tttgattaca tcaacgccgc tgacccgcag
     2461 tggcttcagc tcatgccgta cgacatggtg atcatcgacg gcccgttcca cgacggcttc
     2521 ccgagcccca ccggcggatt tccggtcgct ctcgagcggt acctgaagtc cggagggaaa
     2581 ctcgtctgct tctcgaacct gggggcgtac tacgggatgg gctacctgac ggggcccggg
     2641 acgcgggcgc tgccatcgtc cgttcgctcc tggttcggcg tcgactccgc cacctgcacg
     2701 ggctacggct actattacac caacggcatc ccccacgtcg acagcatctt cgccttccat
     2761 acggcggagc cgatcggcgg cggtttcccg tcgctctcct gcgacaccgc cgccttcccg
     2821 ctcgctgcga actggaccac gttctggccg gaaaccagtc ccccctcggt ggcgaccttt
     2881 acgctttcgt cggatccctc ggccgatgcc gtcgccacgc acgtctttcg gtcgctcgcg
     2941 ccggccacct ccctgcagga gggagggatc gtcggcgtca agagtcgggt ggctttcttc
     3001 gatgacacgt ccgacgtgca cctcttcggt ttccatttgt accacatgga cacgctcgat
     3061 gcccgggcac tgctggcggc cctgcggacg cccaattcgg ccccggccct cgccctgacc
     3121 ggcgagcccg gctacgagcg gaaaggcgtg gtcccgcagt gggggaatcc cggcaccgcc
     3181 ttcgagttcc gcgtcgagtt cagcgatccc gacggcgacc tgccgctgcc cggctacccg
     3241 cgggtgcacc tttacagcgc cctcaccggg ggaccggcgg tcggaagtcc gttcccgatg
     3301 acggcggtgt cgggcgcgcg ctcggcgggc caagtgtacg cggcggtggt gcaagacctc
     3361 ccgcccgatc cctcgtacac gtactggttc gaggcgcgcg atgagcacgg cgcggccgcc
     3421 gccggcgccg tcaccgcggc ccaccggttt tacggtccgg tcgtcacgga tccgggcgga
     3481 gcggtccggt gggtggccgt aaccggcaac gacaccaccg gcacgggcga cgagggcgcg
     3541 ccgctggcct ccgtccagcg cgccgccgat ctcgccaaca acggcgacac catccgcatc
     3601 ggcgacggcg tctatcccgg cgccttcctc agtttctggg gaaaacagct ggtgatcgag
     3661 tcggttcacg gcgccgcggc gaccgtcatc gacggcgggc tgtcacccaa tgtgatcgcc
     3721 tcgaccaaga tcggagtgcc gctggcgggt tttgaggaca gcacgtttgt gctgcgcgat
     3781 ctgactctgc gcaacttcgg aaatgatacg ctcgacggcc gggtgctgga cctgaacggg
     3841 agcagtccgt cgatccgcga ctgccggttt gaggcgacgc gggggcggtc cgccggggcg
     3901 gtggtctgtc ggggcgggag tcccgtcttc gaaggctgtg cctttatcgc caaccgggcc
     3961 gactcggccg gaggggcggt gtggctcgac tcgtgtgaga tccggtttgt cggctgcacg
     4021 tttgccggca acgccgcccc gctgggttcg gcggtgtggg cttccgcgtc gcacgtcacg
     4081 ctcgagcgct gcatcctcgc ctatggccag gggagcccgc cggtggcctt cgacccggcg
     4141 caccccggcg ccgtaacggt ctcctgcacc gatctcttcg gcaacgcggg gggcgactgg
     4201 agcggctgcc tcgccgggct gggaacaggc gacaacctga cccgcaaccc gcagttctgc
     4261 gacacgaccg gcggcgacta ccacctcttc gtgcactccc cctgccgggc ggaaatgaac
     4321 aactgcgccg cgctgatcgg ggcttatgcg gtcgatgagg cctgcgccgg atgctgcgcg
     4381 ctgcgggggg atctcaccgg cgagggttcg gtgacggtcg ccgacatcac cgccctcatc
     4441 cgccaggtct tccgcggcgg ggcggagccg ggttgcccgg agcactccaa cgtcaacgga
     4501 ggcctggggg tcagcctgat cggccccgtg agtgtggtag atatcacgta cctggtcaac
     4561 tatgtcttcc gaggcggatc ggcgcctccc gcctgcggtt tcccgggcgc gcccgaagaa
     4621 gtcgttcctg attttggggc ggtcggcggc gccgctccgg ttccctcgcc gcggccgtag
     4681 ctcaagcggt gaagatgtca taagagaagg cggcctccgc gcgggggccg ccttcgtgct
     4741 ctgcggaccg gtcacacgca agccgggcta catctgcatt gccagcgctt tctgaatgag
     4801 cgtatcgagc gccgggccgc ggcaggtgtc atgcatggcg atcagcgtcc cgtccttgcc
     4861 gatcaaaaaa ggcgagggga tcgagccgac cgcgaaggcc tgggtgatgc cgccggtcca
     4921 gttctgttcg tcgtagacgt ggcgccaggg catcccggcc tcggtgatcc acgcccggta
     4981 ggcatcctgg gtcgtctggt tgggatagtc gagcgagatc gacaggatgt cgaatccctg
     5041 ggatctgtat ttctcgtatg cggctttcag gtagggcagt tccgcgcggc acgggccgca
     5101 ccaggtggcc cagaaatcca gcaggagcac tttgcccctg tagtcctcga gcgacaccgg
     5161 cttgccgtcg atatcggtgg ccgtcagcgg gatcggccag ctcgccttca ccatgtcggc
     5221 gggaaagagc gtcttggcga ccgccatcgc attctcatcg gcggcgttgc gggcggcgaa
     5281 atctttgatc tgcggataga gcgtctcgcg cgcctccccg gcggcgccga ggcgggccgc
     5341 caggatccac ccctccgccc cgccatagcg gcggtaggac gagtccatcc ggccgtagta
     5401 gctctccgct ttggccagcg ccgcctgccc ctcgggcgtg ccggtcgcgg cgggaccgtg
     5461 ctccatgatg gccgccgcca ccccgcggta gcaggcctcc gaggccgatt ctgcgttcgg
     5521 cgaggtcgcc aggtaagcgt cgacttcctt catgatagtc tgcgagaccg ccccccactt
     5581 ctcgtcgtag ggggagttca tgcggcccag cgagatgatg cgctcgaggt cgtagtcgaa
     5641 acggtcgccc aggagcgcct cggtagcggc cagccgcttc gcctcgaggt caaggttggc
     5701 cgcagcggtc tgccaggggt gccagacctg gctgttgacg cggagaatct cctgctgccc
     5761 gtcaaaccac gccaccagcg ccgcggagtc ggtgattccc tgcggcggcg tcacgtcgcg
     5821 cggcaggccc gccgccagca tccccatctt ctccgtatcg agctggcggg ccttgtccag
     5881 gatcgcggcg aagcgcgggt cgtcccgcag cggagcgagg tccgggtcgt tttcgagatg
     5941 atcgactacg gtccagccgc gggtgacggc cgtgtcgaga taggccagcg ccgcgtcggc
     6001 gttccccatc cggctgtagg cgcaggcgag gttgtacagc ccccaccgtc cctcgggaat
     6061 cacctccgcc tgtttccgga acgccgccag cgcggaatcc tgttggcccc gctcgagggc
     6121 gccgtacccc tcgttgctca actggatgta ctgggtgatg agagcctggt cgtaggcagt
     6181 cgggagcgtc aggcgcggcg aatcgtccga cccgcctgag cagccgagaa caagcagcgc
     6241 cagtccgccg agaacgagcg cggcgacggt cgttttgtga ggcacggggc aactcctttc
     6301 ggtggtatga gttcgtcgga gataataaga tgatccccgg ccgccgaggc aagcgcagat
     6361 attgggcgcg ccgccggccc gcccgggccg ctcaggcccg ggccgactgc gtcagcgcgt
     6421 cgaacgccga ccgcaggtcg atcagcagat catcgatatg ttcgatcccg accgacagcc
     6481 gcaccaggcc gtcggtgatc ccccgcggct cgcggatttc cctgggaatc gaggaatggg
     6541 tcatcgccgc cgggtggttc accagcgact ccaccccgcc gagcgactcg gccagcacga
     6601 acacccgcag cgccatcacg aaccgcttga ccgcctcgaa atcgaactgc ggcgcgatcg
     6661 tgaacgccac catgccgccc ggaaggcgca tgcggttcgg caccggccgg ccgtcgagcc
     6721 ccggaaaata caccttctcc accgcctcga ccgtctcgag gtactccgcg atcttcatgg
     6781 cgttgaccga gtgccgctcc atgcgcagcg cgagcgtttt cagcccccgc agcacgagcc
     6841 aggattccca cggtcccaag tgcgccccgc cggcgtactg gttgaaccag agctgctcac
     6901 cgagcgagga atccttcaca atgagcgcgc cggccacgag gtcgcagtgg ccgccgaggt
     6961 acttggtcat cgagtggagg acgatgtcgg cgccgagttc gaggggctgc tggaggtagg
     7021 gcgtggcgaa agtgttgtcg accccgacca ggatcccgcg ctccttcgcg accgccgcga
     7081 ctttctcgat gtccacgagg tgcatgagcg ggttcgtcgg ggtctcgagc cagaagagcc
     7141 gggtgttcgg gcggatggcg ctctcgagat tcttcgggtc gcgcccgtca acataggtga
     7201 aatccagccc gaagcgccgt ccgatccgct cgaactggcg catcgtgccg ccgtagagat
     7261 cgtcgaccgc caccacgtgg tcgccggttt tgagtagttt gagcaccgcg tcgagcgcgg
     7321 ccagcccgga ggcgaacgcc gccgccttcg tcccccgctc gagtttcgcc agcgtctcct
     7381 cgagcgccag ccgcgtcggg tttttccagc gggcgtacac gtagcccgac tcatcccccg
     7441 gatagtcgac acggtaggtc gagctggggt agatcggggt ggtcaccgag cgcgtgccgt
     7501 cctccggcac gcggccggag tggatcgcgg tggtttcgaa ctgccagttc ttgctgcggt
     7561 cgtgagccat aagcgcctct actcgatgaa tcctttctcg cgcatccact cgtcgttgaa
     7621 aattttgctg atatacttgt tcccgtggtc ggggagaatc gccacgagca gtttgtcctc
     7681 ggccagatcg gcttcctgcg cgtagcggag cgccgccagc acgatcccgc cggaggaacc
     7741 gccgccgcag atgccctctt tgcgcgtgag atcgcgggcc atgaggaaac agtcccggtc
     7801 gctcacctgg cagatgtcgt cgatcaccgt cagatcaagc gtcgggcaga ggaagtcatc
     7861 gccgatcccc tccaccaggt aggggtgcgc ctcggggaga gcgccggttt tgaagtagtg
     7921 gtagaagacc gaaccctcgg ggtcggcggc gatcacggcg actttcgggt tctgctcctt
     7981 gaggtagcgg gcggtgccgg agacggtgcc gccggtgccg atgccgccga cgaggacatc
     8041 gatcttcccc ttggtctggc gccagatttc cgggccggtc gtgcggtagt gggcctcgat
     8101 gttgtcggtg ttgaaatact gcccgacgaa gtacgagtgc ggcgtctcgc gggcgatccg
     8161 tttggcggtc tcgtagtagg agcggggatc ctccggtgca accgcggtcg ggcagacatg
     8221 gacctgggcc ccgaacgccc ggagcgcgtt gatcttctcc gccgacatct tgtcggggat
     8281 agtcaggatg gcgcggtaac cgtgggcggc ggcgaacatg gccaccgccg cgccggtgtt
     8341 cccggaggtc gcctcgacaa tcgtcccccc cggcgacagc tcccctttgc gctccgcctg
     8401 ccgcagcagg tagatcgcca tgcggtcttt caccgagccg gtcgggttga gaaactccgg
     8461 tttgaccagg atcgtcgcct tgatccccca ttcccggggc agcacgcgga gccggacgag
     8521 cggggtgttg ccggctgtct cggtcatatc ctcggcgtag gcaatgtccc ggaggcgggc
     8581 cgggagaggt gtggggttgt ggctgttcat ggacaaataa tctctctctt gtctgggtcg
     8641 ttgttttcgg gcgttcgcac ggtccagaac accggagggc cgggaaagtt cgacgccggc
     8701 ccgtagtata gcgggcgcgg agaacctgtg tcaaggccat ggcggcgggc cgcacccgcg
     8761 ggagcgcgcg agacagccga tgcatcgtgc gcgatccggc cgcgcgcgcc ttttccccgc
     8821 aaccgcccgc ccggctgccc gtatattcaa tcacgaacac cggcggaccg cccgtccacg
     8881 aaaccgaccg aactgagagg tgtcgccatg atcgccgacc gtgttctctc cgagcagctc
     8941 gcgctgttct tctatgccga ccccattatg agccgctagc caaccgcggc tccgtcaata
     9001 aacttcctca gggtcccgtt tcccggccaa ggacgcggga cccttcatat tttgttatat
     9061 cattgtcaat caatatagta cgatggtgct tgttgatccg ggaccggtgt gtgtgcgaac
     9121 tctttcctca tttcatcggt gtaaaaaaaa gtttgcttct cggaaaccgg agcacataat
     9181 tagccggttt ccgctagcac cttgaacttg ttctgaggga gtcgaaaatg cgttgcgccg
     9241 agtgtggcga caaaatcacg ggcaagccga tcaagcgcgg gaaccgggtg ttctgctcga
     9301 tcgattgcgc cgagtccgct gcccaggacc ggtacgatga cgaggaagac caggaactcg
     9361 acgatgaatt cgacacgctc gatgaggaag aggtcgactt tgaggaggat tccgaggacc
     9421 gttttttggg aggagaggac gacgagcggt agccggcgga gggcgccggc ggtcgttgcg
     9481 cggggcatct cccgggccga tttttccggg cgctgttcct gcatgggctg cgccccggat
     9541 catcgcgccg gcgggtatcg gtcatgaggg cgagcggggg cgaaaattat atccttgaca
     9601 aaccggattg gcgcgataaa ttaccgggct ggaaactcgg accagttagg acagatttga
     9661 aggtgagcga ccgcgcgccg gataccgacg cgacaaccgc aaaagagaga caaccaagga
     9721 gtgccggctg gatctgggat gcgcggcacg ttaaggatca ctgctaacgc aaccagacgt
     9781 gtgcaagttc gccggggact tgagcatgtc ttttttttca tgcaaaccaa caactcccgg
     9841 cagtgaggta gtgtgatatg ttcaagcgtg tgtcgctggc tctagtggcg ctcgcgatgc
     9901 ttctggcggc ggccggttgc tcgaaagccc cggaagcgga aatgcaggcc agtgaagccg
     9961 ctatgcaggc ggcagtcgcg gccgaagccg agcagtacgc gcccgaagcg ttccaggctg
    10021 cactggacac gctgaattcc gccaaggccc tcaaggccga gcaggacggc aagttttcgc
    10081 tgttccgccg ctacgggaaa gcggccgaga tgttcgtccg cgcccagacc ctctctgagc
    10141 aggccgctac cgctgccgct gaagagaagg agcgcgtgcg ggtgctggtg gccggtctgt
    10201 tgacgcaggc cgacagcgtg ttgacccagg ccacgcagct tgtcgacaag gccccccgcg
    10261 gcaaggggaa caaggctgag atcgagctca tgcggggcga gttgtcctcg gcgaccaccg
    10321 cgctcgccga tgcccgggcc gacttcgacc gcggtcagtt cctggtcgcc aagaccaaag
    10381 tcgaggccgt cagccagcgc ctcaacagcc tgatcaacga agtcaatacc gccttctcgc
    10441 gcacgggcca gcgctgaccg ctgcccccgc gacaacatac ggccccgggg acccttgcac
    10501 agaatcggtc tccggggtgt tcttgtagga gaactccttc gatgacgggg cacgacggca
    10561 ggtgcgacgg acgctgcgca gactgacttt cgccgccgtt ctgatcggcg gcctgggcgg
    10621 gtgcgaggat gcccccgagg ggctgtaccg gcaggccgat tcggctgtgc gccgggtgcg
    10681 cacgcagacg accggcgcct acgccgattc gctgctgcgc caggccgaca gcctgctcct
    10741 atcgggccgt ctggaaatgg cccgacagaa cggccggatc gggccgttcc gggattttgc
    10801 cgccgccgag tcgctgctca cccgggcgct ccaaaccgcc gtggccgcct ccgattcggc
    10861 caaggtccgg cacgaccgcg cccgcgatgc cgcgctcgcc cggcgcgacg agctgctgac
    10921 cgagcttaag acctggcggg aggcgctcaa tgggttcctc cgcctccacg gcgcggaaaa
    10981 acggctgacc aacgccgaac tcggcctcca gatcggccgc cgcctgatcg actccggcaa
    11041 gtacgccgag gccatggagg tgatggatga cgtgcgggat tcgctcggac ggattcgcga
    11101 cattgtcgac gagtacatgc gcgatgagtc ccgccacatc agcacctggc ggcgctgggt
    11161 gaacgaaacg atccaggaat cccgggccac cgggacgtcg gccctcattg tcgacaaagc
    11221 ggcgcaccgg gcctacctgg tgtccagcgg gcggctcgtg aaatcctggc cggccgacct
    11281 cggccacaac ccctcgcgcc agaagctgtt cgcgggcgac ggtgcgacgc cggaggggat
    11341 gtaccgggtg accgaagtgc ggcgccgcgg cagcaagtac taccgcgccc tcatgctgaa
    11401 ttacccgaac gaactggacc gccggcggtt tatggagaat aagaaagccg gggtcatttc
    11461 ctcgggcgcc aaaatcggcg gcctgatcga agttcacggc gagggcggtc agaaccgcga
    11521 ttggaccgat ggctgtgtgg ccctgtccaa cgaggacatc gacgaactgt tccagcacgt
    11581 gcagaaaggg atgcccgtga cgattgtccg gcgctccgac aagtggccat gagatatgtc
    11641 gtgattgtgc tcatcgtcgc gctgacggtc accttcgccg ccatctacct gtcgcctccg
    11701 gacgaggccg acgggtcgcg cgcggcgcag gtggtcggcg acaccgccgc ccggctgccc
    11761 gagggccgct cgctgcgcag ccagctcgcg cttttgcagc gcgacctcaa gcgggtcgcc
    11821 ccccgggagc cgtatatcgt gatcaacacc cacgcgagca agctgtcgct gcgcaccgcc
    11881 gacagcgtgc tgttcgaagc ggtctgctcg gccggttcgg gggccgaact gaccgactcc
    11941 accaccgggc ggcgctgggt gttcgacacc ccgcgcggaa tgtacaaagt cacgagcaag
    12001 atcaaggatc cgtggtggcg caagcctgac tgggcgtttg tcgaagaaaa cgaaccggtc
    12061 cccgaggacg aaaacgagcg gctcgatccg cgcatgatgg gcgagtacgc catcggtttc
    12121 ggcgacggct atttcatcca cggcaccatc tacgagcgcc tcctcggcgt caacgtcacc
    12181 cacggctgtg tccgcctcgg cgcggaggat ctgcgctacc tctacaagaa agtgccgatc
    12241 ggcacgagga tctatgtctt ttagccgccc tcggctgcgc gccctgatcg ccctcggcgt
    12301 cctcgccgcc gccgctatcg cctcctgcga acagcagcaa gcggcctccc agaccaccgg
    12361 cgcggccgac cccggggaag cccgcctcga gcgcgagtac atgcgcgccc tggctgagat
    12421 ggaactggcc cgcggcgacc gcacctacct cgtcatcgac cctggcaggc gggccctgct
    12481 gctgaagctc aaaggcgccg tcgttttctc cgtccccatc accgtcgtcg acgacgacac
    12541 cgcgctctcg gccggcgagg tgaccgaccg gctgcgcgag ttcggcctgg acctgctcgg
    12601 cgacgacgtc gaggtctcgt tcctccgcga ccgttacatg ttcagcagag ccggcgtcat
    12661 ccccgattcg atcctccaga tcatctgcga cgccgtcaac gccgaccccg cgctggtcca
    12721 gcgcttcctc cccgagcgct ttgtcctgga atgggagggg ggcgccgtgc tcgacgtgcg
    12781 caccaccgtc aaggggcagg cgacctcggc gttcggcaac atcatggccg gtctcaagca
    12841 gatcatcaag cgtcccttca ccacccagtt gcgcgtgcag atggacgccg aggcggctct
    12901 caccctgtac tcggtgtgcg agccgggcac gccgaccatc ctcaccgcga tgcagtaacc
    12961 tccccgccgg gccgcgcaaa aaaagccgga accgcgagag tcatgaccat ggcgccgtcc
    13021 gcctgtcccc cggccgttcc cgttaagccg gcggccttcc cgctcattgt tcggggaaca
    13081 tggcctcgta gatctcctgc atctttccgg accgaatctc gcgggtcagt tggtcggttt
    13141 cgcggttgac ccaggcgtgc atcgggtcgt tggccaggag gctgtcgata tacgcgttca
    13201 ccgccgcgtt gccgtaggcc cgcgcctccg gaagcagccc gaagtagaag tgcttggcga
    13261 cctcgtagag accgtgccac caggtgtagt cgggccccat catcgcggcg ccgtggcggg
    13321 cgcggcggcc ctcgtggtgc cagatctccc agtaggtcca ctcgatctcg ttgccgaacg
    13381 aggccgggcg ctccagcagc ccctgggcgc gcacgcgggc gagaatctcc ccggccggcc
    13441 gggcgaactt ctcgttgtac agggccacga ccgcgtcata ctggtagtag tggccgtcga
    13501 cgaacccgcc gccgtggcag ttggcgcaga cgcgcttcat cgccgcccgc ttttcctgcc
    13561 agttctcctt gtgcaccgac actgccggcc gcagcgtcca actgatccgc tccccgacgt
    13621 cgtgggtgcc tggggatgcg ggggtggcgc ctatgtggca ggtggcgcag gtcggggccg
    13681 cggcgtagtc gcgcccgacc acccaactgt cgccggcgag cttcatcttg tcgacgttgg
    13741 tgtagaacgc gttaccgtgc ttcgactcgt cgtagatctc gcgctgcggg tgatccggac
    13801 cgaggtggca cttgccgcac gcctcgggcc ggcgggcctg cgccgccgaa aacgtgtggc
    13861 gggtgtggca agcggtgcac gagcccttgc tgccgtccgg gttgatcctt ccgattccgg
    13921 cattcggcca ggactgccgg gccagccggt tgggccggtt cgggtcgatc tgcaccttcg
    13981 cgccgtggca gttctcgcac ccggcgatcg ccgcgggcgc gccccccgcc acatgggcga
    14041 gataggcgtc gttggactcc agaatctgcc ccgccgtcgc atggtacgaa cgctccacct
    14101 ccgtcgcctc cttctcgtgg caggcgcggc aatcggccgg cgtgaccagg gtggcgatca
    14161 gggccccgtt gtgcgcaaag gcgtccgggt cgccctcggc cgccgcgtgg cagtcccggc
    14221 agcccacccc gtactggccg tggagcgact cctgccactg ccggtggagc cccgcggtct
    14281 gcttctgatg acagtcctcg caggcgtcgg cggacgcggc cgtctgagcc gccgcgccgg
    14341 acgcggctac cgcccacagc aggatgacga tgttgagaaa cctcatgcga tcctcctctc
    14401 cggcggttcc cgccccgaaa gaccgaccgg ggaggagacc gcctcggtga tttgccccca
    14461 acatactcgc gaaggcgccg ggcgcaacag attttggcgc cggtcggggc ccgggccggg
    14521 gcccggcgcc gggggcgcac cgccgcattt attgtttgcc ttggccgcac gttttcgcta
    14581 accctaaggg ggaaccgact gggagcagag gacgtatgcg gacactggtg cagaactggc
    14641 tcatcaatca ccgggtgaac gtcgcggcgg ccgagtggct gtcgtggctg atcgtcggcg
    14701 tcgccgtggc cggcgccatg gtgatcgcca agaccgccgc ccaggtcatc gtgctccgca
    14761 tcgtgcgcta cttcgtcatg cgcacgcaga ccacatggga cgacgcgctg ctccaccggc
    14821 gcttcttcaa ccgcctcgcc cacctcgccc ccggtctcgt actctacttc tgcgccgggc
    14881 tgttcccctc gatccgcgag ggcatcgagc gggcggccat ggtctacatg atcatgaccg
    14941 gcctgttcgc cttctcggcc ttcctgtccg cggtggacga cgtctaccag acgttctcga
    15001 tctcccgcca gcggccgatt cgcggctaca tccaggtcgc ccgcatcatc ctgttcgtct
    15061 ttgtcggcat cgtcgccatc gccactctca tgaaccgctc gccgtggctc ctgctctccg
    15121 ggctcggcgc catgacggcc gtcctgctgc tcatcttccg cgactccatc ctcggcctcg
    15181 tcgcgagcgt ccagctgtcg tccaacgaca tggtccggat cggcgactgg atcgagatgc
    15241 ccaagtacgg ggccgacggc gaggtggtcg atgtcacgct gcacgtcgtg aaggtgcgca
    15301 actgggacaa cacgatcacg aacattccct cgtaccacct catcgccgat tccttcaaga
    15361 actggcgggg gatgcaggag tcgggggggc gccgaatcat gcgcgcggtc tgcatcgacg
    15421 tgtcgagcat caggttctgc acgcccgaga tgctcgatcg tttcgagcag ttccagctca
    15481 ttaccgagta cgtccggcag cgccgcggcg aaatcgagga gtggaaccgc gcccacggaa
    15541 tcgaccgacg gcacccggtc aacggccgca acatgaccaa ccttggcacg ttccgcgcct
    15601 acgtctccgc ctacctgcgc cgccacccgc tcatcaaccc cgacctgccc ctcctcgtcc
    15661 gccaccgcga gccgaccgag aaaggtctgc cgctgcaaat ctacgccttc agccgcgaca
    15721 agatctggac caactacgag gccctccagg ccgacctctt cgaccacatc ctcgctgccg
    15781 tgccgctgtt cgacctgcgc atattccagc tccccggcgg tcacgatgtc cggggactcg
    15841 cggcgcttct tccgcaagat caccggccgg cgccgccggc tgcggccggg ggagcgccct
    15901 cgccctaacc gcggggacac ccatgcgcca acggctgtcc caaggaggtt ttcgcttcta
    15961 tgccccgaat cacccggccc gaacccgaac gggatctttt caagaaccgc tacagccgcg
    16021 cctcggtcac ggcgatgggg cgggcaatca aggcggtctg tccgaccttc gaccttcccg
    16081 gcttcgtcgc ttccgtcatc accgatgatt ttccgcgcct cgagttcaaa gaccgggggc
    16141 gcgccattgc ccgcgccctg gccgaatacc tgccgggcga ttacggccgg gcggtcgacg
    16201 tgctgatcgc cgtcgctccc cgcctcgatg gatttcacaa ctgggcggcc atgacattca
    16261 tcgagctgca gggcctcgcg catttcgagg attcggtgcg cggcatgaag gcgctcaccc
    16321 gctacagcac ggcagaatcg gcgattaggc ccttcctgaa cagcttcccc gaccgcctgc
    16381 tgccgatcct ccacgagtgg gccgccgatc ccgacgagca cgtccggcgg ctggcggccg
    16441 aaggctcgcg gccgaggggc gtctgggttg cgcacatcgg ggcgtacaag aaagacccgc
    16501 gccccgtcct cgccctgctg gagaagctga aagccgatcc ctcgaaatat gtccgcaccg
    16561 cggtcgccaa caacctcaac gacatctcca aagaccaccc cgacctcgtc atcaccgccg
    16621 ccctccgctg gctcaaagac ggggatgccg gcacgacctg gattgtccgc cacgcctgcc
    16681 ggagcctcat caaaaagggc gacccgcggg tatttcccat cttcggcttc accgccaacc
    16741 ccaaaatctc cgtcaccggc ccgcgccccc cgcgccgggc ggtggcgatc ggcgcggacg
    16801 ccgccttccc gttcaccgtc acctcgcgcg cacagagccg ccagaagctg gcgatcgact
    16861 accgcgtcca ctatgtcaag aagaacggga agacgtcgcc aaaagtgttc aagctggccg
    16921 agaagacgat tgcgccgggg gagacgcttg cgctgcaggt gagacagtcg ttcgccgatg
    16981 tgtcggtgag gaaacatgtc cccggacggc atcgtctcga aatcatcgtg aacggcagga
    17041 cagtcgcggc cgcggacttc acgctcgcgt gagaccgggg ccggcgcaca taaaaagagc
    17101 tccgtgccgg gggaggggtg acgacacgga gcggtgatgg ccggtgtcaa acgagttgtc
    17161 aatccgggcc atcgagtctg gtcattttgc cactctccat aacctatcgg caggcgcggg
    17221 agtttcccgc ctccggaaga tttcgaaaaa gaaggccctg gaagtccaca acgccgggcc
    17281 ggacaaagac tgtcagccgc ccccgcgcct tcccgcttgt ttttctgcat cctttccggg
    17341 tatataccaa tcctatggac gagaaagaac aacagctggt gcggcgggcc caggccggcg
    17401 atttcgacgc tttcaccgaa ctgatcgccg cccaccggac caggctgtac aacctggctc
    17461 tcaaaatgac aggcagccaa ctcgatgccg aggacatcgt gcaggatacg ttcctcaagg
    17521 cgatcgacag gatcgaccag ttccgcggcg aagccgcgtt cggcacctgg ctgtatgcga
    17581 tcactctgaa cgagacccgc atggcgctgg cccgcgaaaa acgcgccgac ctccggccga
    17641 tcgaagatta cctccctgcc cacgaccggg atcacggcgg gccgacggtg tacgactggc
    17701 gggaccccca ccggcttttg gagcagggag aattgcgcac cgcgatcgat cgcgcgatcg
    17761 gcgagctccc cctgccgtac cgcgaagcat tcgtcctgcg ctatatcgat gaactcccaa
    17821 taaaagaggt agccgaactg ctcggcgagt cggtcgccgc gaccaagtcc cgggtgctcc
    17881 gggcgcggct ggcggtccgg gaccgcctcg cgaaacagtt cgagggccac aatgggtaag
    17941 aaatgcgctg aatacgtcac cgacctgaac gactacctcg atggcgaaat ctcgccggag
    18001 ctctgcgagg aactcgagcg gcatatcggc cggtgcgaaa actgccggat catggtcgat
    18061 accctccgca agactgtgat cctctgccgg gagggccgcc aagagaagct cccggaatcg
    18121 ctgcaacaga aactgaccga tctgctccgg caggagtggc ggaagaagtt cggtaagtag
    18181 ctgggctgtt gaattttacg tcgcacctga tggggccgga agcccccgga ccggccgcta
    18241 tcggaattcc cccgaaaatt ggaatctttt ttgccttgtt ttgcatctaa cgggtacgac
    18301 ggaaatgact ttcgatcgcc aagctaaaag gtggtggaca atgactctgg aaaacgccgt
    18361 tcgcctctgg gccggtttcg tggttctgct atcgctggcc ctctacttgt gggtctcccc
    18421 gtggtggctg ctcctgacag cctttgccgg gttcaacatg gtgcagtcct cctttaccgg
    18481 tctgtgcccg gcccagtgga tgctcaaaaa gctcttcttc aaggaccagg ccgaggcggc
    18541 cgctcccgcg gggcggcgct cggcccagtc ctcgcgggcg tagccccttt gcggtaagtg
    18601 tccaggcagg cggatcacgg gtcgatccgc ctcctctttt ttaggcttaa ccgttcggcc
    18661 gtcggtgacg atactagata ctatgatgaa acgacatatg cggtttctcc cgataatggc
    18721 gtcggctctc gggctggcgc tcgtgtgggc gttcggctgc agcagcccga ccaaggatgt
    18781 cttcgcgccg gacgaaaaga cataccaggt cgcgggcgcg ctcatcaagg agcccaacag
    18841 caacatcgcc tcggccctga tgcaggtctt caagaacggc gacaccgccg agaccatgac
    18901 gatcctggtc ggcgacgaca ccctc
//
LOCUS       DAOPOE010000063        18404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3647_length_18404_cov_5.178266, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000063 DAOPOE010000000
VERSION     DAOPOE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18404
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_18404_cov_5.178266"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKM94_09225"
     CDS             complement(<1..1174)
                     /locus_tag="PKM94_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD66859.1"
                     /translation="MITVPTNQPTIQAGIEAAADGDTVSVLPGVYAENINFLGKAVRV
                     IAAGAVTDVVLEPAAATAPIVSADSGEGAGAAIKGFTIRNGAGGGVAVANGAHMAIAG
                     NIFSGFTGGAAVLEFSRGSGSTKVCITRNIFTGNSNWACMYGWLDTDSVVILNNTLWQ
                     NTGGVVLMDLSPAVSVVKGNIVANCCDVHGEGAFRSWYVYNDQWNGEPQCGRIEYDGG
                     LNGCFDPRFIDPSAGDFRLREESPCIDAGDPSAVYRDPDGSPNDMGAIPYTGTDYPVP
                     LGLSFGTGRFNNVTPTVTPMFCWSYFDTALTSQAQWQVQVSSDSTWAAVDLWDPGPSF
                     SADTSAVYAGADLRPYRKYYVRVRVGNGGAWGDWRYSAFSPVSDDIFLVPEDWPDLK"
     gene            complement(1337..1855)
                     /locus_tag="PKM94_09230"
     CDS             complement(1337..1855)
                     /locus_tag="PKM94_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD66860.1"
                     /translation="MAVRRIIQLGDPLLWQRSAAVAEPGSAEIAALVRDLEETLAAFR
                     AAHGYGRGIAAPQIGVLHRVIFVRTPDGSFDGPMINPRIIGASGELREWWDSCFSFPD
                     LLVRVARANRIRAVYVGPQGTLRELEAEGELAELLQHEIDHLDGILAVDRALSARSFA
                     TREEWERMGRPL"
     gene            complement(1992..2594)
                     /locus_tag="PKM94_09235"
     CDS             complement(1992..2594)
                     /locus_tag="PKM94_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66861.1"
                     /translation="MRWLLASLLCAAVAAPAGPAAARVHERGTGDDGERSVTVKSFST
                     FEADDARFHLGKDEIVISHLNDPYEEIRITSDRRMYLDDEEIPLTPPQQDLVGEFYQI
                     SYEIRAEAKGIAKEGITLGLKGAKLGLQAVGAAMKMLFTEYDEEQFDRDMEIEAEKLE
                     AHGEQIEKRAKHLEDMVEQWEELGRQMKSEIGPLRNMEWL"
     gene            2799..3650
                     /locus_tag="PKM94_09240"
     CDS             2799..3650
                     /locus_tag="PKM94_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66862.1"
                     /translation="MILPQLGRLDNMRPAALDARTVYIHDDHRWVVPLIFHAQESGRL
                     PVPCKLVCLDRHHDALPPDRERLRALRDARRAGLTGASVLNLCAEVLSPHDDDWIPAA
                     MELGLLSDAVVLGGEGVFSRQALTDFTDHTGARHRIELTGHLGPELAAIGRFGNLLRR
                     GQEELLWNIFDWMVAPDGRLGFRPGAPPLALSIDLDYFVIPWDTFRLPWPEEVYRGYF
                     LTPSDQPALEGATARDILRQLIDRAGLVAVAREAGHCGGESKAAAVFADFNRLLLDGR
                     LRLLPAE"
     gene            complement(3753..3974)
                     /locus_tag="PKM94_09245"
     CDS             complement(3753..3974)
                     /locus_tag="PKM94_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66863.1"
                     /translation="MRYRALAGLLILGAGVLVRGCGKGGSEADGPPDRVPPETKVAEM
                     KDSTRFDPAEPEDTAAHDPEEGEPATPPE"
     gene            complement(4000..4212)
                     /locus_tag="PKM94_09250"
     CDS             complement(4000..4212)
                     /locus_tag="PKM94_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66864.1"
                     /translation="MKKLLVGGLVLLWALTCAFGCGGGEKAEPQTETQMEPAPEQTAP
                     PAAESVAVDTMATPDSAVTDTGALGE"
     gene            4447..5163
                     /locus_tag="PKM94_09255"
     CDS             4447..5163
                     /locus_tag="PKM94_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD66865.1"
                     /translation="MDTSIAPFILAGPIWNIISNSSAFGIFILGVLVFMSLTSWAIIF
                     AKYREFRAVEAENNKFLQTFRRARSLSEAIGQAKSHSSAPVSHIFLAGYNELTEIVGA
                     KNEGGGLQEVKHSLADEDLASVEMVMDKTHNEQIGKLERRVIFLASTANSAPFMGLLG
                     TVVGIMDAFWSIGERGSASLAVVAPGIAEALLATIVGLGAAIPAVIAYNWANSKLKYA
                     NDFSQNFILEFLARVRREVR"
     gene            5163..5609
                     /locus_tag="PKM94_09260"
     CDS             5163..5609
                     /locus_tag="PKM94_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD66866.1"
                     /translation="MPRRGRTKLPYRPMADINIANLVDVVLVLLIIFMISAPLLQSGI
                     EVDLPKTRAAAIEEETEGIVLTIDKKGGIYINDVWSRLDNFEKNLQDEMAAKGTRSVY
                     LRGDSVVAYGTAIGVIGRLKEMGIESIGLVTAPPQDNAAGRRGRRR"
     gene            5623..6354
                     /locus_tag="PKM94_09265"
     CDS             5623..6354
                     /locus_tag="PKM94_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD66867.1"
                     /translation="MATRDIIFSAALHTVVIVVTLVSIPLAQKRELPFADIIRVQLTA
                     PAQLPGRQPAAIPAPPVPQPEVDANPDLPVEPPKPVEKEAVIDKKPEKKPEKKPQPKP
                     QPQPEESAPAASSAAGESPSGAAGSGPQTNAPVGEGSPFGGATIDNPSFTYPWWFTQA
                     FNKIASGFHVRQAFPPDVTCVIYFEVIRSGRVIELRVDQSSGYPDFDDACMTAVSRAT
                     PFPPLPREFTDEVLGITVPFKPSQR"
     gene            6358..7686
                     /gene="tolB"
                     /locus_tag="PKM94_09270"
     CDS             6358..7686
                     /gene="tolB"
                     /locus_tag="PKM94_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOD66868.1"
                     /translation="MRLSFPTILAAALLSFASSARSQSGESVRQIMFDTIQARAVEPL
                     KVGVDQMKYIGIQYITAEDSSLMLHATAVVQRDIDFYADFDLVPLDTFYLRVYEITEV
                     DLLGWERLGAERLVRLEAEFPGSNIRIYWRLYDVTHNQEIAKGQVEYHRAYWRELSHD
                     VANEVVYRLTGDPGIFRTKIAYVKQLGKDLKEIFIADYDGANERQLTKTGSICLSPAF
                     TPDGREIYFTSFLGGKADLYKVGVDDGKVVKIAAYEGINSAPAVAPDGRKIACVLSLD
                     GDAEIYTLDIGGRIMSRLTRLRSIESAPSWSPDGRRIVFSSDRTGQPQLYVMNDDGLN
                     ERRLTFRGSYNDSPVWAPQGDRVVFVTRTDAGFNIASVDVGGSEYHMLTNLGNNENPH
                     FAPDGKHVIFTSSRLGGTDLYTMDAGGRNQRRLTQTKNVSNPAWGPFPRR"
     gene            7870..8388
                     /gene="pal"
                     /locus_tag="PKM94_09275"
     CDS             7870..8388
                     /gene="pal"
                     /locus_tag="PKM94_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOD66869.1"
                     /translation="MKKFAWITMIGLALVALTLTSCGKKQVAEEAPVTQPVVTPADTA
                     TPPPPPPPPPAPKTLTNEQLEVVYFDFDKYNLRPDAKSSLDRNFALLQEFPNAIVELQ
                     GHCDERGTVEYNLALGERRAKSVQTYLIGLGIDPARLKTVSFGKERPAVPGHDESAWA
                     KNRRCEFHVLSQ"
     gene            8413..9207
                     /gene="ybgF"
                     /locus_tag="PKM94_09280"
     CDS             8413..9207
                     /gene="ybgF"
                     /locus_tag="PKM94_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOD66870.1"
                     /translation="MLTRTKHSAAAVLATAAAALGIALTGCVTPQRIDRLEEQIKVTQ
                     RQNIDTQNMLRNIDTTVAGEAADNRRLRADLSVTIDQLQMQIDQLQQNYSDLLQRIDE
                     LYNLLEAKGQLRSSSGAVTPPGPLPGTAPATPPAAATPDAECGTMYDDAFVLVRRAEH
                     EQAIQAFQAFLTACPSHELAENAYYWIGESYYSLGKYPEAIAQLELLLKTFKASPNTS
                     RALFKLGRSQEELGKKKEARQTFQRLVDDHPGTLEAEQAKERLKNL"
     gene            9212..9745
                     /locus_tag="PKM94_09285"
     CDS             9212..9745
                     /locus_tag="PKM94_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66871.1"
                     /translation="MPADQVRLRIVAPTEPILPGRGFYQLEEDALYVQVGVFSSRRRF
                     FSYLENDLLRLDFDRAGRLIFFELSVPRRQWPVDPGLELPPAIEPADLRWLDFRGPIS
                     VPALATDPPRTLLRLTFAAGPPVRHYFLGQDVFASVDDCDALVHLWIRDIVDDLAGQE
                     IGAFRKKHRSHRSFFAG"
     gene            10083..12092
                     /gene="ligA"
                     /locus_tag="PKM94_09290"
     CDS             10083..12092
                     /gene="ligA"
                     /locus_tag="PKM94_09290"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOD66872.1"
                     /translation="MTTPPRKIIDELEKLRREIRRHDVLYYVEDRPEITDAEYDRLFD
                     RLLAIERDYPDLVAPDSPSQRVGAAPSKRFEPVRHRLPMLSLQKVTDRAGFEDFDRRV
                     RQILETGEVAYLVEPKLDGLAVELVYRDGLFVEGSTRGDGETGENITPNLRTIRNLPL
                     RLSHGAARKYPLLEVRGEVILKRSAFERLNRRLREAGQPMLANPRNGAAGSLRQLDPK
                     ITAARPLVFFAYGVSERTLPGLPGQSATAAFLRREGFSVPDFMELVSGVDAVDRAFAR
                     LAASRPELDYEIDGMVVKVDSYAHQEALGQISRAPRWAVAWKFSAEQAETILEGVEFS
                     VGRTGTVTPVARLRPVAVGGVTVSSATLHNEDEIARLDVRVGDTVIVQRAGDVIPAVV
                     SVVADKRPAHTRPIVFPKACPSCTEPLVRPEGEAAYRCQNIACPAQTERRLIHFASKN
                     CFDIEGLGVKLAGQLIAAAVVRDPADVFYLTKDQLLPLDLMADKRAENLLNAIDRSRR
                     AELPRVLHALGIFGVGETAARILAERFETLDALAAASVEDLQSTPGIGPVLARNITDF
                     FGNEGNRRMIEKMRAGGVAFPPCRSRRAGGPLAGKTFVITGTLSRPRPQIQKLIEEHG
                     GKVASSVSAGTDYVVCGENPGSKRDRAEKLKIPIIDENELSRLLD"
     gene            complement(12278..12409)
                     /locus_tag="PKM94_09295"
     CDS             complement(12278..12409)
                     /locus_tag="PKM94_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66873.1"
                     /translation="MREDFDHDEKKWLIVVGVLVLVLLLVATYQFWPEALRVFAWPG"
     gene            complement(12424..13251)
                     /gene="murI"
                     /locus_tag="PKM94_09300"
     CDS             complement(12424..13251)
                     /gene="murI"
                     /locus_tag="PKM94_09300"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOD66874.1"
                     /translation="MTQHEAPIGIFDSGVGGLTVAREVFGLLPAEDIVYFGDVGRYPY
                     GGRSKEIITQFTRQDIALLLEQQVKFIICACNTASSVALEELRPHYSTAMIGVIEPGA
                     RAAAERTRNGRIGVIGTNATIGSNAYARVIHELDAKLKVFSLACPLFVPLAEEGYIDK
                     EATWLIARDYLKTMQDVDVDTLVLGCTHYPLLRHVIQDVMGEKIELIDSGQETARVAL
                     RYLAAQGLLNPTAREAPARAGVHKFFVSDVPEKFGSIATRFLGRHIENITRVDISEY"
     gene            complement(13322..14854)
                     /locus_tag="PKM94_09305"
     CDS             complement(13322..14854)
                     /locus_tag="PKM94_09305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD66875.1"
                     /translation="MSRIFRLGVITAILLAAAAAAQEQIEVRVEGGTESIRAIQRDGV
                     TFLSLSELAEVVGGSLDWETVGHTVVYQGERDRFRFLIGSPWMQLNDSAYNLVHAVEL
                     VEGQLFVPAATFTPYLDRIVPQQATWAEEARRLRLDTDVYNVTDIAFSPKANGLLIEI
                     FLSTALSYEAFTTQGNYLNVSIRNGVLNRTQILSRRDRRLMYDLNAYQVTGAAQISIR
                     LMDEIKEWHHRLLQNPPRIQISIADQNFVLAPAEPDTPPAVGPDNRIDVIVIDAGHGG
                     KQYGAIGPTGAREKDVALDIARNLARLIRKDKQFKVIMTRDRDTTVTLEERAKIANDA
                     GCDLFISIHANSSTKRHVAGWNVFFLAPARNDSARAVAQFENSAFLRDQAPPPDSEEA
                     GGSEEALFGDPVLSILNEMIMTEFQSESHDFAMMCDREFRRALKTTARGVDQAGFFVL
                     NRVYAPSVLIETGFISNRNEERLLRTPEYQEAVAKALYDAVKRFKAKYERFAEEGERS
                     AG"
     gene            complement(14872..15333)
                     /gene="smpB"
                     /locus_tag="PKM94_09310"
     CDS             complement(14872..15333)
                     /gene="smpB"
                     /locus_tag="PKM94_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOD66876.1"
                     /translation="MKEAADRKYIVRNRKARHDYEIKETYEAGIALVGSEVKALREGK
                     VNIADAYAAVEAGEVILKNLHISPFKMARDNHDPTRPRRLLLHKREIRRMRAATEQKG
                     FTLIPLGIYFRKNLAKVELAVAVGRKKYDKRQAIAAAEAGRRIKQALRKDL"
     gene            15563..15964
                     /locus_tag="PKM94_09315"
     CDS             15563..15964
                     /locus_tag="PKM94_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD66877.1"
                     /translation="MSHKINISSAKVLVIDDEPEITEIVETFLTEAGYYVMVENQPFR
                     AIEKATAFNPDVILLDIMMPGMDGYDVCEALKKVPRLAGTPIIFLTGKDRNDDMGRSF
                     KSGGDMFIKKPFSCERLLEILNIVLLSTGRH"
     gene            16043..16642
                     /locus_tag="PKM94_09320"
     CDS             16043..16642
                     /locus_tag="PKM94_09320"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOD66878.1"
                     /translation="MRLIKRYSNRRLYDTRASRTITHRELAALVRRGEALRVVDTVTG
                     SDITAEVLGRIAFRETAAWTTARESQALFTRIIELGGERTMSILKNTVLASIGAFEVS
                     KARAEKIVDELIKRGEVEKGDKTKAVMEILDRAEKSTAKVYGKVAAEVRKVQQEVAKY
                     SDQAKKYKLVKTEDLRALEEKVERLTRLIEDMEARSGKS"
     gene            complement(16817..17935)
                     /locus_tag="PKM94_09325"
     CDS             complement(16817..17935)
                     /locus_tag="PKM94_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOD66879.1"
                     /translation="MAKYKIAWMPGDGVGNDVMEAARIVLDRMKLDAEYPHADIGWEF
                     WRTEANPLPDRTIKVLKECQCALFGAITSKPRDEAEQELSPSLRGKGHVYSSPIVRLR
                     QEFNLRTNLRPCKGYAGNPLNYKEGIDLIVFRENTEDLYAGVEFHPLPDEVRAAIKKH
                     NKSMAKFDRHAGKDIAVSLRINTREGCRNIVTDAFEYAKKCGRKSVTVVEKPNVIRET
                     SGLMVRTAREVAKGYPGIELWETNIDAMCMWLIKNPLDYGVIVTTNLFGDIVSDLCAQ
                     LVGGLGFAASGNIGDDFAVFEPTHGSAPKYAGQYKVNPMAMLLSVKLMLDWLGETATA
                     DKLERAIAAVVKEGKVRTYDMGGSNSTLEVAEAVAAKL"
     gene            complement(17960..>18404)
                     /locus_tag="PKM94_09330"
     CDS             complement(17960..>18404)
                     /locus_tag="PKM94_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66880.1"
                     /translation="MEKIDQANGWAFAADLGATVRVRPDLTFGAAVTGLGSDLTLQVA
                     GEDASRPISLPTTWRVGGAYVYDRVAGAADFVYLDDAAHGHFGVEVRAHELLQFRTGY
                     MAGYDSKGFTAGASFVQRNVTIDYAFVPYRNDLGTTHMFNLRLTL"
BASE COUNT         3174 a         6862 c         5032 g         3336 t
ORIGIN      
        1 ccttgagatc cggccaatct tccggcacca ggaagatgtc atcggaaacc ggagagaatg
       61 ccgagtagcg ccagtccccc catgcgcctc cattaccgac gcgcacgcgc acgtagtact
      121 ttcggtaggg gcgaagatcc gctccggcat agaccgcgct cgtatctgcc gaaaagctcg
      181 gcccggggtc ccacaaatcg accgctgccc aagtgctgtc gctgcttacc tgtacttgcc
      241 actgcgcctg cgaggtgagc gcagtgtcga agtagctcca gcagaacatg ggcgtgactg
      301 tcggcgtcac gttgttgaac cgccccgtcc cgaaactcaa gccaagagga accggatagt
      361 ccgtgcccgt atagggaatc gcccccatat cattcggcga accgtccgga tcacggtaga
      421 ccgccgatgg gtccccggca tcgatacacg gcgactcctc tcgaagtcgg aagtcccccg
      481 ccgacggatc aatgaacctg ggatcgaaac agccgttcaa gccgccgtcg tactcgattc
      541 ggccgcattg cggttccccg ttccattgat cgttgtatac ataccagctg cggaatgccc
      601 cctcgccgtg gacgtcgcag caattggcca cgatattgcc cttcactacc gaaacggccg
      661 gcgagagatc catcaggact actcccccgg tattctgcca gagcgtgttg ttcagaatga
      721 ctacggaatc ggtgtcgagc cagccataca tacacgccca attggagttg ccggtgaaga
      781 tgttcctggt aatgcagacc ttggtgctcc cgctgccgcg gctaaactcc aacactgcag
      841 ctccccccgt gaaaccactg aagatattgc ccgcgattgc catgtgcgcg ccattggcca
      901 ccgccacgcc gcctccggct ccgttgcgaa tagtaaagcc tttgatcgct gcccccgctc
      961 cctcgcccga gtccgccgac acgatcggcg cggtcgctgc tgccggctcg agtaccacat
     1021 ccgtgaccgc ccctgccgcg atcactctca ctgctttccc aagaaagttg atattctccg
     1081 cgtacactcc cggcagtacg gacaccgtat ccccatccgc cgcggcctca atccccgctt
     1141 gaattgtcgg ttgattcgtc ggaaccgtga tcatccccgc ccccgctgtg cctgccgcaa
     1201 ccggcaatca cgcacttgcg aacaatgcgc gggcgttcat tcacatcacc ctttctccgc
     1261 cgtcgacctc gtcatgatat cggtaatata agcacattca cgattgtgtc aacgtgggtt
     1321 cgtgcgcccc ccgccgctac agcggccggc ccatccgctc ccactcctcc cgcgtcgcga
     1381 atgatctcgc cgacaacgcc cggtcaaccg ccaaaatccc gtccaggtgg tcgatctcgt
     1441 gctgcagcag ttccgccaac tccccctccg cctccagctc ccgcagggtc ccctgcggcc
     1501 cgacatatac cgcccgaatc cgattcgccc gcgccacccg cacgagaagg tcggggaaac
     1561 tgaaacacga atcccaccac tcccgcagct ccccactcgc cccgatgatg cgcggattga
     1621 tcatcgggcc gtcaaaactc ccgtccggcg tccgcacgaa gatcacccgg tgaaggaccc
     1681 cgatctgcgg cgccgcgatt ccccgtccgt atccgtgagc ggcccggaac gccgccagcg
     1741 tctcctccag gtcgcgcacc agcgccgcga tttcggccga gcccggctcc gcgaccgccg
     1801 ccgacctttg ccacaggaga ggatccccca gttgaataat ccgtcgcacc gccatttttt
     1861 actccaagaa acctggtata cggcgccccg gccgtcccgg caagccccgc ccttcgcccc
     1921 caaagcagaa cgcctgcagc ggtcgtgtga ccgtacaggc gttcttcaaa ctgatgggaa
     1981 atgcgccggc tctacagcca ctccatattg cgaaggggac cgatctcgct tttcatctgc
     2041 cgccccagct cctcccactg ctcgaccatg tcttcgagat gtttcgcccg cttctcgatc
     2101 tgctccccgt gcgcctccag tttctccgcc tcgatttcca tatcgcggtc gaactgctct
     2161 tcatcgtact ccgtaaacag catcttcatc gcggccccca cagcctggag tcccagtttc
     2221 gcccccttga gacccagcgt gattccctct ttggcgatcc ctttcgcctc cgcgcggatc
     2281 tcatagctga tctggtaaaa ctcccccacc aggtcctgct gcggcggcgt gagcgggatc
     2341 tcctcgtcat ccaggtacat tcgccggtcc gacgtgatcc ggatctcctc gtaaggatcg
     2401 ttcaggtggc tgatcacgat ttcgtcctta cccaggtgga accgcgcgtc atccgcctcg
     2461 aacgtgctga aactcttgac tgtgaccgac cgctccccgt cgtcccccgt cccgcgctcg
     2521 tgcactctcg cggccgccgg tcctgccggc gcagccacgg ccgcgcataa tagactcgcc
     2581 aacaaccatc gcatcgggct ttcctccaca aatagcgttc gttccgccat tgcactatac
     2641 gagccggttc ccgcaaggtt gcacccggcg gccgcgctcg ccgggccggg gcccgccccg
     2701 ggtccacggg ctgtcccgcc gagcgccgct tttttgttgc ccgccgcctt cccggccgcc
     2761 gtatcatacg gcaacgacac actgccgcga ggtatgccat gatcctcccg caactcggcc
     2821 ggctcgacaa catgcgcccc gccgcgctcg acgcccgcac cgtctacatc cacgacgatc
     2881 accggtgggt ggtgcccctc attttccatg cccaggagag cggccgcctc cccgtcccct
     2941 gcaaactcgt ctgccttgac cgccaccacg atgcgctccc cccggaccgg gagcgtttgc
     3001 gggccctgcg cgatgcccgg cgcgccgggc tcaccggggc ctccgttctg aatctgtgcg
     3061 cggaggttct ctctccccac gacgatgact ggatccctgc cgccatggag ctggggcttc
     3121 tctccgacgc cgtcgtgctc ggcggcgagg gggtgttttc gcgccaggcc ctcaccgact
     3181 tcaccgacca caccggcgcc cgccatcgca tcgagctcac cggccacctc ggacccgagc
     3241 tggcggccat cggccgtttc ggcaacctcc tccggcgcgg gcaggaggaa ctcctatgga
     3301 acatcttcga ctggatggtg gcgcccgacg gccgcctcgg cttccgcccc ggagctcctc
     3361 ccctcgcgct ctccatcgac ctcgattact tcgtcatccc gtgggatacc ttccgccttc
     3421 cctggcccga ggaggtctac cgcgggtact tcctcacccc atccgatcag cccgctctgg
     3481 agggcgccac cgcccgggat attctgcgtc agctcatcga ccgtgccggc ctggtggcgg
     3541 tcgcccggga ggcgggccac tgcggcggag agtcgaaagc cgcggccgtg ttcgccgatt
     3601 tcaaccgcct cctgctggac ggtcgcctgc gcctcctccc tgccgagtga cctcggccgg
     3661 ccggcgctcc ccaaaataca acagcccccc gaatcctttc ggggggctgc cctttgggcc
     3721 cgctgaagct caggacgctt cgcggctttt cgttattcag gcggcgtcgc cggctctccc
     3781 tcttcagggt cgtgagccgc cgtatcttcc ggctcggccg ggtcgaaacg cgtgctgtct
     3841 ttcatctccg ccactttggt ctcgggcggc acgcgatcag ggggcccatc ggcctccgat
     3901 ccccccttgc cgcacccgcg caccagcacg cccgccccca ggatcaacag ccccgccagc
     3961 gctcggtacc gcatgtcccc gatccctcga cctctcccgc tactcgccca gggcgcccgt
     4021 gtccgtcacc gccgagtccg gagtcgccat cgtatctacc gccacgcttt ccgctgccgg
     4081 tggcgccgtt tgctccggag ccggctccat ctgggtctcg gtctgcggct ctgctttctc
     4141 gccgccaccg cacccgaaag cgcaggtcag cgcccacaac aggactagtc cgccgacaag
     4201 tagcttcttc atctgcccgc tctcctctcc ttgagtccgg ttaacgtatg ctgtctctcc
     4261 ttttccacct gcaaagctgc ttgagatcga agattttccc ccgcccgtgc tcagtcaagt
     4321 ttttaattcc gcctgtttta gttgaccctg ccggatcctc ccgatatgtt gcggctccga
     4381 tttcggaaac gcggatggat tccgtccgcc tcgaaaactt taggtgaaac gggtgtttaa
     4441 aagaatatgg atacttcgat tgctcccttc atcctcgccg gcccgatctg gaatatcata
     4501 tcgaattcgt ccgcatttgg catctttatt ctgggcgtcc ttgtgttcat gtcgctgacg
     4561 tcctgggcca tcatcttcgc caagtaccgc gagttcagag cggtcgaggc cgagaacaac
     4621 aagttcctcc agactttccg tcgcgcccgc tctctgtcgg aagctatcgg ccaggcgaaa
     4681 tcgcactcct ccgcccccgt ctcccacatc ttcctcgccg gctacaatga acttactgag
     4741 atcgtcgggg ccaaaaacga aggcggcggt ttgcaggaag tcaaacacag tctggccgat
     4801 gaggatctgg cgtcggttga gatggtcatg gacaagaccc acaacgagca gatcggcaag
     4861 ttggagcggc gcgtcatctt cctcgcctcg actgccaact ccgctccgtt catgggctta
     4921 ctcgggactg tcgtcggtat catggatgcc ttctggtcga tcggcgaacg cggctccgcc
     4981 tccctcgcgg tggtcgcccc cggtatcgcc gaagctctcc tggccacgat tgtcggcctc
     5041 ggcgccgcca tcccggctgt catcgcctac aactgggcga acagtaagct gaaatacgcc
     5101 aacgacttct cccaaaattt catcctcgag ttcctcgccc gggtgagacg ggaggtccgc
     5161 tgatgccgcg ccgtggccgc acaaaactcc cctatcgccc gatggcggac attaacatcg
     5221 ccaatctcgt ggacgttgtc ctcgttctgc tgatcatctt catgatttcc gcccccctcc
     5281 tgcagtccgg catcgaggtc gatctgccca agacccgcgc cgccgccatc gaagaagaaa
     5341 ccgagggcat cgtcctcacg atcgataaga agggcggcat ctacatcaac gacgtctggt
     5401 cccggctgga taatttcgag aagaacctcc aggacgagat ggccgccaag gggactcgtt
     5461 cggtttacct ccgcggcgat tccgtggtcg cgtacggcac cgccatcggc gtcatcggcc
     5521 ggctcaaaga aatggggatc gagtcgatcg gcctggtcac cgccccgccg caggacaacg
     5581 ccgccggacg gcgcggccgc agacgatagg agcaccccga ttgtggcaac ccgggatatc
     5641 atcttctcgg cggcactcca caccgtggtc atcgtcgtga ccctggtgtc catcccgctg
     5701 gcccagaaac gcgaacttcc cttcgccgat atcatccggg tccagctcac cgctcccgcc
     5761 cagttgcccg gccgccagcc ggccgccatc ccggccccgc ccgtccccca gcctgaggtc
     5821 gacgccaacc cggatcttcc ggtcgagccg cccaagccgg tcgaaaagga ggcggtcatc
     5881 gataaaaagc ctgagaagaa accggaaaag aagccgcagc cgaaacctca gccccagccg
     5941 gaagagtccg cccccgcggc ttcctccgcc gccggagaat cgccctccgg cgccgccggg
     6001 agcggcccgc agaccaacgc cccggtgggc gaaggctccc cctttggcgg cgccaccatc
     6061 gacaacccca gtttcaccta tccctggtgg ttcacccagg ccttcaacaa gattgcctcc
     6121 ggtttccacg tccgccaggc ctttccgccc gatgtcacct gcgtcatcta tttcgaggtg
     6181 atccgctccg gccgcgtcat cgaactgcgc gtcgaccaat cctccggtta tccggatttc
     6241 gacgacgcct gcatgaccgc ggtcagccgc gccaccccct tccccccgct tccccgtgaa
     6301 ttcacggacg aagtcctggg catcaccgtt cctttcaaac cttcgcagag gtaagacatg
     6361 agactgtcgt ttccgaccat cctcgccgcc gcactcctct cgttcgcgag ttccgcccgc
     6421 agccagtccg gcgaatccgt ccgccagatc atgttcgaca cgatccaggc ccgcgccgtc
     6481 gaacccctca aggtcggcgt cgaccagatg aaatacatcg ggatccagta catcaccgcc
     6541 gaggactcct cgctcatgct ccacgccacc gcggtcgtcc agcgcgacat cgacttctac
     6601 gccgacttcg acctcgtccc cctcgacacc ttctacctcc gcgtgtacga gatcaccgag
     6661 gtcgacctcc tcgggtggga gcgcctgggc gccgaacgcc tcgtccgcct cgaggccgaa
     6721 ttccccggct ccaacatccg catctactgg cgcctctacg acgtcacgca caaccaggag
     6781 atagccaaag gccaggtcga ataccaccgc gcctactggc gcgagctgtc tcacgacgtc
     6841 gccaatgagg tcgtctaccg cctcaccggc gaccccggca ttttccgcac caagatcgcc
     6901 tacgtcaaac agctcggcaa agacctcaag gaaatcttca tcgccgacta cgacggcgcc
     6961 aacgagcgcc agctcaccaa aaccggctcc atctgcctct ccccggcttt cacgcccgac
     7021 gggagagaga tctacttcac ctcgttcctc gggggcaagg ccgatctgta caaggtggga
     7081 gtcgacgacg gcaaggtcgt gaaaatcgca gcctacgagg ggatcaactc cgcgccggcg
     7141 gtggctcccg acggccgcaa gattgcgtgc gtgctgtcgc tcgacggcga cgctgagatc
     7201 tacacccttg atatcggcgg gcggatcatg agccgcctca cccgcctccg ctcgatcgaa
     7261 tcggctccga gctggtcgcc cgatggccgg cggattgtct tctcctccga ccgcaccggc
     7321 cagccccagt tgtacgtgat gaacgacgac ggcctgaacg agcgtcgcct caccttccgc
     7381 ggcagctaca acgactcccc cgtctgggcg ccccagggcg accgcgtcgt cttcgtcacc
     7441 cgcaccgatg ccgggttcaa catcgcttcg gtcgatgtcg gcggctccga gtaccacatg
     7501 ctgaccaacc tcggcaacaa cgaaaacccc cactttgccc cggatggcaa gcacgtgata
     7561 ttcacctcct cgcgcctcgg cggcaccgac ctgtacacga tggacgccgg cggccgcaac
     7621 cagcggcggc ttacccagac caaaaacgtc agcaaccccg cctgggggcc cttcccgcgg
     7681 cggtgagcgg cccccgcccg gcccgcgcgc gagaaccatc ggctgcgcgc gcggtgtaat
     7741 ttgcgacacg accccgggaa tgcttttttt tcgttttatg ttgaccgttt ttcggcaaac
     7801 ggatagattg cgtgcaaagt gactaaggat agcaacagta ataattccct ttagggagga
     7861 agagaagaca tgaagaagtt cgcgtggatc accatgatcg ggctcgcgtt ggtcgccctg
     7921 accctgacgt cgtgcggcaa gaagcaggtg gccgaggaag cgccggtgac tcagccggtc
     7981 gtgaccccgg cggacaccgc caccccgccg ccgccgccgc ccccgccgcc cgcccccaaa
     8041 acgctcacga acgagcaact ggaagtcgtg tactttgatt tcgacaagta caacctccgg
     8101 ccggatgcca aatcgtccct cgaccgcaac ttcgccctcc tccaggagtt ccccaacgcc
     8161 atcgtcgaac tccagggaca ctgcgacgag cgcggcaccg tggaatacaa cctggctctc
     8221 ggcgaacggc gcgcgaaatc cgtccagacc tacctcatcg gcctcggcat cgaccccgcc
     8281 cgcctcaaaa ccgtcagctt cggtaaagag cgcccggccg tccccggaca cgatgagagc
     8341 gcctgggcca agaaccgccg ctgcgagttc cacgtcctct cgcagtagca tgaaccgatt
     8401 ggtcgtgctg tcatgctgac tcgcaccaaa cactctgctg ccgccgtcct cgcgacggcg
     8461 gcggccgctc tgggcatcgc gctgaccggc tgcgtcactc cccagcggat cgaccgtctg
     8521 gaagagcaga tcaaggtgac gcagcggcag aacatcgaca cccagaacat gctccgcaac
     8581 atcgacacga cggtggccgg cgaggccgcc gacaaccgcc gtctccgcgc cgatctctcg
     8641 gtcactatcg accagctcca gatgcagatc gaccagctcc agcaaaacta cagcgacctc
     8701 ctccagcgga tcgacgagct ctacaacctg ctcgaggcca aaggccagct ccggtcctcc
     8761 tccggtgcgg tcaccccgcc ggggccgctc cccgggaccg ctccggccac gccgcccgcc
     8821 gcggcgaccc ccgatgccga gtgcggaacg atgtacgacg atgctttcgt cctcgtccgc
     8881 cgcgccgaac acgagcaggc cattcaggcc ttccaggctt tcctcaccgc ctgccccagc
     8941 cacgagttgg ccgaaaacgc ttactactgg atcggcgagt cctattactc cctcggcaaa
     9001 taccccgagg ccatcgccca gctcgaactc ctcctgaaaa ccttcaaggc gtcccccaac
     9061 accagccgcg ccctgttcaa actcggacgc tcccaggagg aactcggtaa gaagaaagag
     9121 gcccgccaga ctttccagcg cctcgtcgac gatcaccccg gcaccctcga ggccgagcag
     9181 gccaaggagc ggctcaagaa cctctaggcc tatgcctgcc gaccaggtca gactgcggat
     9241 tgttgccccg accgaaccca tcctcccagg tcggggtttt tatcaactgg aggaagacgc
     9301 gctctatgtc caggtcgggg tcttctccag ccgccgtcgc ttcttctcct accttgagaa
     9361 cgacctcctc cgtctcgact ttgaccgcgc cgggcgcctg atctttttcg aactctccgt
     9421 cccccgccgc cagtggcccg tcgatcccgg gctcgaactc ccgcccgcca tcgagcctgc
     9481 cgacctccgc tggctcgatt tccgcggccc tatttctgtc cccgcactcg ccaccgaccc
     9541 gccccgcacc ctcctccgcc tcaccttcgc cgccgggccg cccgtccgcc actactttct
     9601 cggacaggat gttttcgcct cggtcgatga ttgcgatgcc ctggttcacc tctggatccg
     9661 cgacatcgtc gatgacctcg ccggccagga gatcggcgca ttccgcaaaa aacaccgctc
     9721 ccaccgctct ttcttcgcgg gctaggcgtc gtcttcaccc cttggcgcac gaccggtccg
     9781 gtccccccgg attccttgta cgtcgcgacc ctcgcggtcc ggacacccct gccatcgacg
     9841 aagaccatcc tcctgatctc ttgcacgtca ggacccttgt ggtcctgtca tttttgccat
     9901 cgctgcgagc ggtcccgtca ggaccctgaa ggtcctgacc tacaaaagcc ccgtcgcgcg
     9961 ggttctggcg aaactctgtc ctgtcctaag actttgtgat cgccgtgcgt ggaccttgaa
    10021 tgtcagcccc ggccccccaa aaatgattga cacctccccg accgttgtct caaattacgc
    10081 gcatgacgac cccgccccgc aagatcatcg atgaactcga aaaactccgc cgcgaaatcc
    10141 gccgccacga cgtcctctac tacgtcgagg accgcccgga aatcaccgat gccgagtacg
    10201 accgcctctt cgaccgcctc ctcgctatcg agcgcgacta ccccgatctg gtcgccccgg
    10261 actctccatc gcagcgtgtc ggggccgctc cctcgaaacg tttcgaaccg gtccgccacc
    10321 gcctgcccat gctctcgctg cagaaagtga ccgaccgggc cgggttcgag gatttcgatc
    10381 gccgtgtccg ccagattctc gaaaccggcg aggtcgccta tctcgtcgag cccaagctcg
    10441 acggcctcgc ggtggagctg gtttaccgcg acggcctctt cgtcgagggt tcgacgcgcg
    10501 gcgacggcga gacgggcgag aacatcaccc ccaacctccg caccatccgc aacctccccc
    10561 ttcgcctctc ccacggcgcc gcccggaagt acccgctgct ggaggtgcga ggcgaagtga
    10621 tcctgaagcg ttccgctttc gagcgcctca accgtcgcct ccgcgaggcg ggccagccga
    10681 tgcttgccaa cccgcgcaac ggcgccgccg gctcgctccg ccaacttgac ccgaaaatca
    10741 cagccgcccg tcctctcgtc ttcttcgcct acggcgtctc cgagcgcact ctccccggcc
    10801 tgcccggtca gagcgccacc gcggcgttcc tgcgccgcga gggcttctcc gtgccggatt
    10861 tcatggagct cgtctccggc gtcgacgccg tcgaccgggc cttcgcaagg ctggccgcct
    10921 cgcgtcccga gctcgattac gaaatcgacg gcatggtggt gaaagtcgac agttacgccc
    10981 accaggaggc gctcggccag atctcccgcg ccccgcgctg ggcggtcgcc tggaaattct
    11041 ccgccgagca ggccgagacg atcctcgagg gcgtcgagtt ctcggtcggc cgcaccggca
    11101 ccgtcacccc ggtggcccgc ctccgcccgg tggcggtcgg cggcgtcacc gtctccagcg
    11161 ccaccctcca caatgaggat gagatcgccc gcctcgacgt tcgcgtcggc gacaccgtga
    11221 tcgtccagcg cgccggcgac gtcatccccg cggtcgtgag cgtggtcgcc gacaagcgcc
    11281 ccgcgcacac ccggccgatt gtctttccca aagcttgtcc ctcctgcacc gagcccctcg
    11341 tccggccgga gggagaggcc gcctaccggt gccagaacat cgcctgcccg gcgcagacgg
    11401 aacgccgcct catccatttc gcctcgaaaa actgtttcga catcgagggc ctcggcgtga
    11461 aactcgccgg ccagctcatt gccgccgccg tcgtccgcga ccccgccgac gtcttctacc
    11521 tcaccaagga ccagctcctt cccctcgacc tgatggccga caaacgcgcc gagaacctcc
    11581 tcaatgcgat tgaccgctcc cgccgcgccg agctcccccg cgtcctccac gccctcggca
    11641 tcttcggcgt cggcgaaacg gccgcgcgca tcctcgcgga acgcttcgag accctcgatg
    11701 ccctcgccgc cgcctcggtc gaggacctcc agtccacgcc gggcatcggt cccgtgctgg
    11761 cccggaacat taccgacttc ttcggcaacg aggggaatcg gcgcatgatc gagaagatgc
    11821 gcgccggcgg cgtcgctttt ccgccctgcc gcagccgccg cgccggtggt ccgctcgccg
    11881 gcaaaacctt cgtcatcacc ggcaccctct cacggccccg cccgcagatt cagaaactca
    11941 tcgaagaaca cggcggcaaa gtcgcgtcct cggtgtcggc cggcaccgac tacgtcgtct
    12001 gcggtgaaaa cccgggctcc aagcgcgaca gagccgagaa actcaagatc ccgattatcg
    12061 acgaaaacga gctctcccgc ctcctcgatt agcttcggcc caaccccccg catcctcggc
    12121 ggccccccga tcttttgcag gtcacgaccc ttgtggtcct gacattcctg ctctcgacga
    12181 cgatagcccc ggccgggcca tgaaggtcct ggcctgcctt accaacgaat attcgtcgct
    12241 cgccccgtgc gcacgcgcgg gacagcccgc cttcgcctca ccccggccag gcgaacaccc
    12301 gcagcgcctc cggccagaac tggtaggttg ccaccagcag caggaccagc accaaaaccc
    12361 cgaccacaat cagccacttc ttctcgtcat gatcgaaatc ttcccgcatc gctcgatcct
    12421 ccctcagtat tcgctgatat cgacgcgcgt gatgttttca atatgccgtc ccaggaagcg
    12481 cgtcgcaatc gacccgaatt tctccggcac gtcggagacg aagaacttgt gcacgcccgc
    12541 gcgggccggc gcctctctcg cggtcgggtt gagcagcccc tgggcggcga gataccgcag
    12601 cgccacgcgc gccgtctcct gccccgaatc gatcagctcg atcttctccc ccatcacgtc
    12661 ctggatcacg tggcgcagca gcgggtagtg tgtgcatccg agcacgagcg tatccacgtc
    12721 gacatcctgc atcgtcttca ggtaatcccg cgcaatcagc caggtcgcct ctttgtcgat
    12781 atacccctcc tccgcgagcg gcacaaagag cgggcaggcc agcgagaaca ccttcagctt
    12841 ggcgtcgagc tcgtggatga cgcgggcgta ggcgttggaa ccgatcgtgg cgttggtgcc
    12901 gatcaccccg atacgcccgt tcctggtccg ctcggccgcc gcgcgcgccc ccggctcgat
    12961 cacgccgatc atcgcggtgc tgtagtgcgg ccggagctcc tccagcgcca ccgacgaggc
    13021 cgtgttgcag gcgcagatga tgaacttcac ctgctgctcc agaaggagcg caatatcctg
    13081 ccgggtgaac tgggtgatga tctccttcga ccgcccgccg tagggatacc gtccgacatc
    13141 gccgaaatag acgatgtctt cggccggcag gaggccgaac acttcgcgcg cgaccgtcag
    13201 cccgccgacg ccgctgtcaa agattccgat cggagcttcg tgctgtgtca tggccggaat
    13261 ttacacgccg gcggtcgacc gcggaacatt aaaaacgcgc gccccggccg gccgccgccg
    13321 ctcacccggc cgagcgctcc ccctcctccg cgaaccgctc gtatttcgcc ttgaaccgct
    13381 tgacggcgtc gtacagcgcc ttggccaccg cctcctggta ctccggggtc cgcagcaggc
    13441 gctcctcgtt tcggttcgag atgaatcccg tctcgatcag caccgagggc gcgtacacgc
    13501 ggttgagcac gaaaaacccc gcctggtcga ccccccgcgc ggtcgtcttg agcgcccgcc
    13561 ggaactcccg gtcgcacatc atcgcgaaat catgcgattc gctctggaat tccgtcatga
    13621 tcatctcgtt gagaatcgac aggacgggat ccccgaacag ggcctcctcg gagccgcccg
    13681 cctcctcgct gtccggcggg ggcgcctggt cgcgcaggaa cgcgctgttt tcgaactggg
    13741 ccaccgcccg cgccgagtcg ttccgcgcgg gggccaggaa gaaaacgttc cacccggcca
    13801 cgtgccgctt ggtcgagctg ttggcatgga tgctgatgaa caggtcgcac ccggcatcgt
    13861 tggcgatctt cgcccgctcc tccagcgtca ctgtcgtgtc ccggtcccgc gtcatgatca
    13921 ccttgaactg cttgtccttc cggatcagcc gggccagatt gcgggcgatg tcgagggcca
    13981 cgtctttctc ccgcgccccg gtcggcccga tcgccccgta ctgcttcccc ccgtgcccgg
    14041 cgtcgatcac gatcacgtcg atcctgttgt ccggacccac cgccggcggc gtgtccggct
    14101 ccgccggcgc cagcacgaaa ttctggtcgg caatcgaaat ctgtatgcgc ggggggttct
    14161 ggagcagacg gtggtgccac tccttgatct catccatcag ccggatcgaa atctgggccg
    14221 cccctgtcac ctggtaggcg ttcaggtcgt acatgagccg gcggtcgcgc cgcgacagaa
    14281 tctgcgtccg gttgagcacc ccgttgcgga tcgagacgtt gaggtagttc ccctgcgtcg
    14341 tgaacgcctc gtacgacagc gccgtgctga gaaagatctc gatcagcagg ccgttcgcct
    14401 tcggcgaaaa agcgatgtcg gtgacgttgt agacgtcggt gtccagacgg aggcggcgcg
    14461 cctcctccgc ccaggtcgcc tgctgcggca caatccggtc caggtacggc gtgaacgtcg
    14521 ccgccggcac gaacaactgc ccctcgacca gctccaccgc gtgcaccagg ttgtacgccg
    14581 agtcgttcaa ctgcatccag ggcgacccga tcagaaagcg gaaacggtcc cgctccccct
    14641 gatacacgac cgtgtgcccg accgtctccc agtcgagcga cccgccgact acctccgcca
    14701 gctccgaaag cgataggaac gtcactccgt cgcgctggat cgcccggatc gactccgtcc
    14761 ccccctcgac ccgcacttcg atctgctcct gcgcggccgc ggcggcggcc aggagaattg
    14821 ctgtgatgac accgagccgg aatattctac tcatacactc tgccgcgcgg tctacaggtc
    14881 cttgcgcagc gcctgcttga tccggcgccc cgcctcggcc gccgcgatcg cctgacgctt
    14941 gtcgtacttt ttacgcccga ccgccaccgc cagctcgact ttcgcgaggt tcttgcggaa
    15001 atagatcccc agcgggatga gcgtgaaccc cttctgctcg gtggccgccc gcattctgcg
    15061 gatttcccgc ttgtgcagca gcagccggcg cggccgcgtc ggatcgtggt tgtcccgcgc
    15121 catcttgaag ggtgagatgt gcagattctt caggatcacc tcgcccgcct cgaccgccgc
    15181 gtaggcgtcc gcgatattca ccttcccctc ccgcagcgct ttgacctcgc tacccaccag
    15241 ggcgatgccc gcctcgtacg tctccttgat ctcgtagtcg tggcgggctt tccggttgcg
    15301 gacgatatat ttccggtcgg cggcttcctt catgggcgta cgataggacg gccgaccagc
    15361 gggggcaatt aaaatcgggt ctcccccgcc gtttgcgtcg tccccgcacc gccgccgcgg
    15421 ccgcccgcgc cgccgcgcgc cccccgctcc tccggggaaa ttgccgcccc ccggtaaagt
    15481 gttccccccc gacagccgaa aacaaatatg tagcgcccga cggcccctcg gccccgggcg
    15541 gagacttcac aaaggtggac aaatgtctca caagatcaac atatcgtcag ccaaggtgct
    15601 ggtcatcgac gacgaacccg aaatcaccga aatcgtcgaa accttcctca ccgaagccgg
    15661 ctactacgtc atggtggaaa accagccctt ccgggccatc gaaaaagcca ccgccttcaa
    15721 tcccgatgtc atcctgctgg acatcatgat gcccggcatg gacgggtacg atgtctgcga
    15781 agcgctcaag aaggttccgc gcctcgccgg cacccccatc atcttcctca ccgggaaaga
    15841 ccgcaacgac gatatggggc gatccttcaa atcgggcgga gacatgttca tcaagaaacc
    15901 gttctcgtgc gaacggctcc tggaaatcct caacattgtc ctgctctcca ccggccggca
    15961 ctgacccgcg gccccgcttt ctccggccgc cgatttttgt tgctcttttt ggtgcgccgc
    16021 actataatag ggcaggtggt ctatgcggct catcaaacgg tacagcaacc ggcgcctcta
    16081 cgacacccgc gccagccgaa ccatcaccca ccgcgaactc gccgcgctgg tgcgccgcgg
    16141 cgaagcgctc cgggtggtcg acaccgtcac cggcagcgac atcaccgccg aggtgctcgg
    16201 ccgcattgcc ttccgcgaaa ccgccgcctg gaccacggcc cgggagtccc aggctctttt
    16261 cacgcggatc atcgaacttg gaggtgagag aaccatgagc atcctcaaga acactgtcct
    16321 cgcctcgatc ggcgcttttg aggtcagcaa ggcccgcgcc gaaaaaatcg tcgacgaact
    16381 catcaagcgc ggcgaggtcg agaagggcga caaaaccaag gcggtcatgg aaatcctcga
    16441 cagggccgag aagtcgaccg ccaaggtcta cggcaaagtc gccgccgagg tcaggaaagt
    16501 gcagcaggaa gtcgccaagt acagcgacca ggccaagaag tacaagctgg tcaaaaccga
    16561 ggacctccgc gccctcgagg aaaaggtcga gcggctgacg cggctgatcg aggacatgga
    16621 agcccggtcg ggcaaatcct gaccgcccgc ctcagcccga cccccataac gacgccggcg
    16681 gccgcagggc cgccggctca gtgtacctgg tctctccaca gtgagacgag accgctagat
    16741 gatacgcgcg cgcccggggc gcccgcaacc ctccgccgcg gcctgccggc cgccccgtcg
    16801 ggtgcgggag gccgtcctag agtttggccg ccaccgcctc cgccacctcg agcgtgctgt
    16861 tgctcccgcc catgtcgtac gtccgcactt tcccctcctt gaccaccgcc gcgatcgccc
    16921 gctccagctt gtcggccgtc gccgtctcgc ccagccagtc gagcatcagc ttcaccgaca
    16981 ggagcatcgc catcgggttc accttgtact gccccgcata cttcggcgcc gacccgtgcg
    17041 tcggctcgaa caccgcgaaa tcatcgccga tatttccgct ggccgcgaac cccagcccgc
    17101 cgaccaattg cgcgcacagg tcgctgacaa tgtcgccgaa caggttcgtg gtgacgatca
    17161 ccccgtagtc gagcgggttc ttgatcagcc acatgcacat ggcgtcgata ttcgtttccc
    17221 acagctcgat ccccgggtac cccttggcca cctcgcgcgc cgtccgtacc atcagccctg
    17281 aggtctcccg gattacgttc ggcttctcga ccaccgtcac cgacttccgc ccgcacttct
    17341 tggcgtactc gaaagcgtcg gtcacgatgt tccggcaccc ctcgcgggtg ttgatccgca
    17401 gcgacacggc gatatccttg ccggcgtgcc ggtcgaactt cgccatgctc ttgttgtgct
    17461 tcttgatcgc cgcccgcacc tcgtccggca gcgggtggaa ttccacgccg gcgtacaggt
    17521 cttccgtgtt ctcccggaac acgatgagat cgatcccctc cttgtagttg agcgggttgc
    17581 cggcgtaccc cttgcacggt cgcagattgg tccgcaggtt gaactcctgg cgcagacgca
    17641 cgatcggcga cgagtacacg tgccccttcc ccctcaacga tggactcagt tcctgctccg
    17701 cctcatcgcg cggcttggag gtgatcgccc cgaacagcgc gcactgacac tccttcaaca
    17761 ctttgatcgt ccggtccgga agcgggttgg cctccgtccg ccagaattcc cacccgatgt
    17821 cggcgtgcgg gtattccgca tcgagcttca tccggtccaa gacgatccgc gccgcctcca
    17881 tcacgtcatt gccgacgccg tctcccggca tccaggcgat cttgtactta gccatactct
    17941 cctgtccctg atcctttctc tacaacgtaa gcctcagatt gaacatatgc gtcgtgccga
    18001 gatcattccg atatggtacg aatgcgtagt cgatcgtgac gttgcgctgc acgaacgagg
    18061 cccccgccgt gaaacccttg ctgtcgtagc ccgccatgta ccccgtccgg aactgcagca
    18121 gctcgtgcgc ccgcacctcg actccgaaat gcccgtgggc ggcgtcgtcg aggtacacga
    18181 aatcggccgc ccccgccacg cggtcgtaga cgtaggcgcc gcccacccgc caggtcgtcg
    18241 ggaggctgat cggacgcgag gcgtcctctc cggcgacctg cagcgtgagg tcggaaccca
    18301 gcccggtcac cgccgccccg aacgtgagat ccgggcgcac cctcaccgtc gcccccagat
    18361 cggccgcgaa cgcccacccg ttggcctggt cgatcttctc catg
//
LOCUS       DAOPOE010000064        18048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3731_length_18048_cov_4.872673, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000064 DAOPOE010000000
VERSION     DAOPOE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18048
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_18048_cov_4.872673"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKM94_09335"
     CDS             <1..488
                     /locus_tag="PKM94_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66881.1"
                     /translation="KMLDTTAAAAAAADPAADSLRQKLGGLMGNMTYTVQRTDSTRKI
                     DQWNTRKFIITMTLGMGATVTSEAWATTDAKIDWSMYRALMLQAMAFLPNAGDMYKEG
                     MKIEGIAVLVNVSTAMMGMEMKSVQRLLAFEENKPAPAGVYDIPEDYRKENSPYLILG
                     Q"
     gene            1132..6873
                     /locus_tag="PKM94_09340"
     CDS             1132..6873
                     /locus_tag="PKM94_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD66882.1"
                     /translation="MKRACACLLLLVLALAAGRESRSSPVPPASLEIPARPDSSLLPY
                     EVEVRRKMAPDPSAPQVVTPFQIHRGGPRPGDSILVVFAYNPSVTTDNPAMFSYQDLT
                     TGHVINDNPVRLTIADFRLCPDAARGDTVVVGAGFRRDSAFAVKFGPAEDSVAWLFLA
                     AGEDHTGDGHWQPSITCVGLADYDYDARHEILFYLNAVRDLVPRVLVCIEVETFAVEW
                     TLPVATGVMAGQVHLWGNPADPGLIFVSYNSKQGVSDSLFSDSWAYVTAVDRTGRVRF
                     NRVIAAEHGGIAIIPAEQPGEYYLGHSTPLTEPADTLAFLQPVRRVSRISTDGAVLDS
                     SPPLSAPVRALWVPPGAGDSGVWVLTQDGTVSRFDRRLRLMARSAPSHLRSFQGTIDL
                     PGRSEPAWLFGSELGAVVMTADLKPLAVLPAVGEYRALIAFDPEAERASYVFSGGGGA
                     LIAEFDHRPLWSVASNFLWRYHLHLSAVAIALLVTLVIVNRRRLQVQRLLRQREADFL
                     AFCQASPDLVFRINRDGVITDFHAREHADLLLPPEVFLNRPLSESLPPAVAGPAMEAI
                     GRALDTGAMESLEYDLDQGGERRHWELRMAPVDDCQVLTVIRDWTDRARAENELRESE
                     ERFRMLFHGSRDAYVIADAETGKVIDLNARAEALIGRPRDRLIGRHVSEMAPPDIRAN
                     FGERYKRHLEIASTGKWINGVVCGEDGRRCPVEISHTLIEIGGRSVLHTTLRDVSERL
                     ATEAALRRSEDRLAALVANIPAAIYRCLPGEARLMEFVSNEITTVTGYESAVFEPGGG
                     TPYASIVHRDDRSRVSEVIAAAVAGRRPFSAEYRIVRADGEVRWVHERGQAVAGPDGT
                     VAFLDGAIYDITEQRAAIDALHESEEKMRAQYKGIPVPTYTWQRAGDDFVLADYNDAA
                     VAITGGTIALKAGIRLSLLYADRPDLRADIARCFDEQTTIRREMDYTFQSLDRTVSLI
                     VHYVYVPPDLVMVHTEDVTDRVRSRQQLATRLRYEEGLVGCSLTLLQTPVSAGALNDA
                     LYHLLTALDSCRVYVYQNFTDPELGLCAREVYEVCRPGVRPTLGRPELQRRPYAPQFA
                     SMRDRLAAGRPVGGVVAEMSEPVRSVNAPCGVLSVLTLPVFVRGQWYGFIGFDDTRSA
                     RRWSEEDVRLLRAAAAMVGSYLEMIEANRELAAERDFTRMVLQTANSLICCLDERGCI
                     TVFNDELERVTGYRREEVLGRSWHELFLPEHFHHPGLVRFGQWVRDHPADRYERALLT
                     RTGEERIILWSNSMLEDPHTGAVTAIAIGHDITDRKHMETAVRESEEKYRAVMEQSAD
                     FIYLAQADSGRIIEANRSLREMLGYSVEEMCGISLFDFVDHDSGEVREGFRKVLAGGL
                     PFLRERYYRTRDGRRIPVEVGVNEIVYGGHKVLCIVSRDISERKAAEESVRQQEEKYR
                     LLVENVRASIILVDAEGYFLFVNQNAAQAVGRRPEELIGRTEWDIFPEDLADRQMSNV
                     RRVIAEDKEFISEAPVYLRGEWRWFYNNVQPYRDLRGRTVAALVIAHDITMAKRVEEA
                     LRESEERFRELADLLPQTVFETDVVGRLTYSNRHGFESTGYTSADLEAGLTVDQLFLE
                     SDRAEVRANFAKRLAGENPQRYEYTHLRKDGSTFPVLIYSSRIVRDGLPAGLRGVVVD
                     ITERKRAEEEVRRANLLRYQQMREIAGGVSHEIYNSLYPAIATLEKLRGLLAASDTTD
                     LARTERLMQLADRAVRRAISLTETVTQYARLESQRRVERVRLAGVIQEIVEQNRHAAE
                     ELAATITADIPADLFVTVGKSYLYSLFNNLVINALDAVAEVTERAVSIAARPDDGRVR
                     IVVRDTGPGIPFEIQQRAFEPFFTTKLDRGTGLGLAVAKRIVELYDGQIGLESALDRG
                     TTFIILLPGGSHL"
     gene            6888..8240
                     /locus_tag="PKM94_09345"
     CDS             6888..8240
                     /locus_tag="PKM94_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD66883.1"
                     /translation="MSKSGQILIVDDDRMVLEAMLESFTDDYAVLTALSGQEALERIK
                     EHPEIDTIILDIRMARMDGLKAANLIKDIAPEIPIIFHTGFAGNYSEEDIDRDYHPFD
                     FIGKNEPPSRLRRAVKNAVQYHRLGNTGSDLIDAARERYGMVGKSRDMRQIYQRIEQI
                     GPTTSKVMILGPTGSGKELVARALHARSRRADREMMVVNCSQKDPTMVESELFGHTEG
                     SFTGAVANRVGYFEAADGSTLFLDEIGELDPATQAKILRAIEYGEVTPVGTCVKRSVD
                     VRVICATHLDLEEQVRSGAFRMDLYYRLKGVTIRLPALRERREDIPELLDYFIERTCR
                     INGVGLKVFEPSARALLVEFDWPGNVRQLSDTVESLIQLSPGYYITYHDVANHLGYES
                     SAAPNGNSGLAEQVWEFKRMKIVQALDRARGNVIAAGEELKIDPANLRKMIKRLNIES
                     "
     gene            complement(8322..8762)
                     /locus_tag="PKM94_09350"
     CDS             complement(8322..8762)
                     /locus_tag="PKM94_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD66884.1"
                     /translation="MYKRVLLAAVGLVLMGAVSASAAGERIDWQVIAAGGGNGSSTNY
                     RLSGTVGQTAAGAGSSANYRVNQGFWQDFGSGGCCVLRGDFNEDGAVKVSDLTGLINY
                     LFRGGVAPICLPHGDTNADGAIKVSDLTLLINYLFRGGPAPAAC"
     gene            9360..11648
                     /locus_tag="PKM94_09355"
     CDS             9360..11648
                     /locus_tag="PKM94_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD66885.1"
                     /translation="MKARNVLCLAVGVCLLALVGLVQAETAPPSAALNTKGLAIGQVR
                     TVHFGGPQTLSIAGEQMTFTAPEPAPEQDPDFVYTLTDKRQGGENIATAFVIPGMPFT
                     DTGTTVGYADDYSEACDGVNYSHVGDVVFAYAPPVKQLVDIKLCKSDYYTRLWVYQTD
                     ETNMIACNRFNTPAVCSPNPRSALMDVQMLPGFTYYIVVDGDHQVTPNEGQYIIECSA
                     TLVPDSVDRWPALADNDNGLLLLGYSNNNGVDSQMFWQESLDDGLTFEPAGYWTNPGW
                     QKYASVDYWGQDSIAYGTFVPSPLNNNGGSTYLHRFYNIANSAGWSLSYWNWTSYGWR
                     NMRMADIACDDGFIFSPNPGDHRFGVIAMVHSSTYSSGGVVVGTDAPHLFYQTDSSNY
                     ATISWYNNLNGCRSTTCDIDRVTKYSYAVYDWLDTSKTPDQWALFIRGDVFGNPDDTL
                     FSGGVTYSLDPGEHIGYPAVAANNGTVLVACEFYTEATPADHDIIFFYSGAADGAYDN
                     MLSGVVVATADDERYPEIQHVTGNTFIVSYIANNQLYMTVTEDGGTTWSTPSVISGAD
                     HVVNEYRAADIADFGKKIIWEYQPNHPADTTIMLHWAETGVVQDADGDGVPDEDDNCP
                     AIANPLQEDADLDGIGDVCDACTDTDNDGYGNPGYPANTCALDNCPNVANPGQQDTNG
                     DGIGDACCCLDNRGNVDGDPDDLIKVGDLTYLVEFLFRFGPVPPCPNEADIDGDGNTK
                     VADLTYLVAFLFRFGPAPATCP"
     gene            12002..13696
                     /locus_tag="PKM94_09360"
     CDS             12002..13696
                     /locus_tag="PKM94_09360"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HOD66886.1"
                     /translation="MAKTVTNSELRFDVGANPIRYCGMQKVYPFSRDRVVEINLHHQH
                     VLEMYDLELVGGTAICLADRLNERSNLGALRNRKLREAAVASAALSAVAVSLHGRGSL
                     AALPKDKQTKEVANELKRANDRTSAQVMAEVLQTTTETLSIGEEVLIESTITEGVRAK
                     PGKEVGGNPTIAVGALFGKKEHRARYGLDLGKDVTLLSMGNDVIDGTTKSVKGLHSSM
                     TCMFLIESQVKRHLPDIYVQRWMGGAHFDEFNPREGTLLDAARVIADSYGFADLSELS
                     AFFIDRSRHYPAWDTLGAAGIGIPYDADGDLFPALLLGLEGMRFPNGRHMNSMIGEIG
                     GSAEWVVGALPLVWRGGQALGMLTSHSSLSRRDLSPEDLWNSRFHYTEDELIMIQDAR
                     FQQKPYFSVKDIIENPHAGGISAFGAITDSVYLPFMKGVQFDPDKGKISVWTLVINSL
                     GLMEVWEMHFACRTSLAHTRALIASPKERLAGLRGKALEAAIGKMLDDPREERRYRIF
                     FNNEYYPALVPIREKVALLGQALEGLIARGALNDQDREIIDLTARLAPEWWVENGR"
     gene            complement(13761..13845)
                     /locus_tag="PKM94_09365"
     tRNA            complement(13761..13845)
                     /locus_tag="PKM94_09365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13809..13811),aa:Leu,seq:gag)
     gene            complement(13904..14347)
                     /gene="secG"
                     /locus_tag="PKM94_09370"
     CDS             complement(13904..14347)
                     /gene="secG"
                     /locus_tag="PKM94_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD66887.1"
                     /translation="MFFIALVVFHVLVCVALIIVVLLQSSKGEGLAGTAFGGGLGGAV
                     FGGRGAGSFLSKATTVLAILFMVNSIGLAFMSSSRTGLRSQSTTAGPTESVVTREAQR
                     AREQAVQQQQQTQQQQPSADSLLNVNPNPGTQPGQGQQTAPGDGQ"
     gene            complement(14362..15120)
                     /gene="tpiA"
                     /locus_tag="PKM94_09375"
     CDS             complement(14362..15120)
                     /gene="tpiA"
                     /locus_tag="PKM94_09375"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD66888.1"
                     /translation="MRSRKLLAGNWKCNGTIPETEKLLAALLTGRWDPQRAEALVCPP
                     YTSLHLAAKLLHGSPIKVGAQDMSAHENGAFTGEISADMLLTVGATHVILGHSERRQY
                     HRETDQSVNAKLKLALRKGLIPIVCVGEELEQRQNGRTEEVVGAQVRIACAEIESGSL
                     ERIVIAYEPVWAIGTGKTATPAMAQEVHAFIRDRLAAQYGQPSADRIPILYGGSVKAD
                     NAADLLAEPDIDGALVGGASLKAADFLAIINAAR"
     gene            complement(15117..16334)
                     /locus_tag="PKM94_09380"
     CDS             complement(15117..16334)
                     /locus_tag="PKM94_09380"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD66889.1"
                     /translation="MRKVTVHEVNFRSQKVLVRVDFNVPLDNGGVTDDRRIREALPTI
                     RKIIGDGGRAILASHLGRPKGQPVAGMSLRPVARRLAELLGREVTFAEDCVGPEAASV
                     VNKMKDGDVVLLENLRFHAKEEKNDPEFAQKLASLADTFVNDAFGSAHRAHASTEGVT
                     RYFRQSIAGLLMEKELQYLGQALENPKRPFAAILGGAKISGKIDVIENLMAKVDLLLI
                     GGGMTFTFSKAMGYPIGDSLLEEDKVALAKEILGKIKKSRAELVFPVDAVVAQEISDT
                     AETRTVPIDRIPDGLKGLDIGPQTIRLFEEKLAQANTILWNGPMGVFEHRPFAEGTFA
                     VARMLARRTGEGAITVVGGGDSASAVKEAGVEDGLTHISTGGGASLEFLEGKELPGVA
                     ALTNAGKAEAVRR"
     gene            complement(16360..17346)
                     /gene="gap"
                     /locus_tag="PKM94_09385"
     CDS             complement(16360..17346)
                     /gene="gap"
                     /locus_tag="PKM94_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOD66890.1"
                     /translation="MRVAINGFGRIGRLVFRAAQDTSIEFVAINDITDAATLGHLLKY
                     DSVHRSYPGEVKAEGTNLKVDGRTIPVMAVLDPAQLPWKELKVDVVLECTGKFTKKDD
                     AMKHVAAGAHKVLISAPAKGHDGTFIPGINSDLYDRDKHHVISIGSCTTNCLAPVAKV
                     LLESFGIVNGFMTTIHSYTADQRLMDAPHKDLRRARSAALSMVPTTTGAAKAIAEVIP
                     ALKGKMDGCAIRVPTPNASLVDLAVTLEREATVQEINAAMKKAAESPALRRALQYMED
                     PIVSVDVIGNPHGSVFDSQLTMVHGRLAKVFSWYDNEWGFSNRMVDMMLMML"
     gene            complement(17343..>18048)
                     /locus_tag="PKM94_09390"
     CDS             complement(17343..>18048)
                     /locus_tag="PKM94_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66891.1"
                     /translation="VFAVLAAAAWGGARAAGEEPTTSQLFLQSHQAGVRLGPWFNLGD
                     MPPARGETSGGEEVFKTNFHDASFLFEGFYAHRLTPALMGELSLAILNRGSVTFTAGN
                     QTNVGNVLVYAGLLQAKVYPLAGGGASVQPYLTAGGGLYVGRRSVQFTSVPEGYYYPN
                     IDEETAAKISYAVGGGIDWPIASQIGLEINAKYCPIPFGKPLMTIEDYDGLAVSIGIK
                     YLYRPAKDETDSRRRP"
BASE COUNT         3156 a         6113 c         5660 g         3119 t
ORIGIN      
        1 gcaaaatgct cgacaccacg gcggcggccg ccgccgccgc cgacccggcc gccgactccc
       61 tgcgccagaa actcggcggt ctcatgggca acatgaccta caccgtccag cgcaccgatt
      121 cgaccaggaa aatcgaccag tggaataccc gcaagttcat catcacgatg accctcggga
      181 tgggggcaac ggtcacgagc gaagcctggg ccacgaccga cgccaagatt gactggagca
      241 tgtaccgcgc cctcatgctc caggccatgg ccttcctccc caatgccggc gacatgtaca
      301 aagaaggcat gaagatcgag ggcatcgccg tcctcgtgaa cgtctcgacc gccatgatgg
      361 gtatggagat gaagtcggtc cagcgactgc tcgcgttcga ggaaaacaag ccggccccgg
      421 ccggggtcta cgacatcccc gaggactacc gcaaagaaaa cagcccctac ctgatccttg
      481 gtcagtgacg tcagacgcgc gcctggggag aacgagactc cggggctctg cctgtgcggc
      541 ggaaagaacc atgaccggga gcaatgcgag cggggggcgc gaaatctgcg cccccgcagc
      601 ggcgcgcacc ggccgaggcc caagacgcaa ccgggtcgag gacccgccgg ttgggaggga
      661 ctctccaaga ggccttcggg gccggcgagc gcggatgtac cgcttgtgcg atgtaactcg
      721 aaggcggcgt gcgggattcc gatcgttcat gtcatgcgat gacaggcggc gcgccctgag
      781 tgtttttcgg tcaaagagaa ctccgacaac ggcgtgagga acttttgact gcgaccggcc
      841 gttatagcag gtgagcagtg aagtagcgca gattttttct tgacaaaggg atgaactttc
      901 tgttaatcag ttgttgacaa gggcgtgaaa aagcttataa tgcagaggca atagaaaagc
      961 cccctagaaa ccctgcctgt cccccctccc gtgtgctcaa gcccaacagg cagggtttcc
     1021 tcctttatgt gaagaccctt cgggataatc cgaagggtct tcccgtttcc ggcgcgatcg
     1081 ggggtccggc tttcaatttg gctttttgac cgttctgcat atcttgatgg tatgaagcgc
     1141 gcgtgcgcat gccttctgct tctcgtgctc gccttggcgg ccggccgcga atcgcgctca
     1201 tcgccggtgc cgccggcgag tctggagatt cccgcgcggc ccgattcgag cctgctgccg
     1261 tacgaggtgg aggtcaggcg gaagatggcg ccggatccct cggcgccgca ggtcgtcact
     1321 ccctttcaga ttcaccgcgg aggaccgcgg cccggcgaca gcatcctggt cgtgtttgcc
     1381 tacaatccct ccgtcaccac cgacaatccc gccatgttct cctaccagga tctcacgacc
     1441 ggccacgtca tcaacgacaa cccggtgcgc ctgacgatcg ccgacttccg tctctgcccg
     1501 gacgccgcgc gcggcgacac ggtggttgtg ggggccgggt tccgccgcga ttccgcattc
     1561 gcggtgaaat tcggaccggc ggaggattcg gtggcctggc tgtttctggc cgcgggcgag
     1621 gaccatacgg gcgacggcca ctggcagccg tcgatcacct gcgtcggtct cgccgattac
     1681 gactacgacg cgcggcacga gatcctcttc tacctcaacg ctgtgcgcga tctggttccg
     1741 cgtgtcctcg tgtgcatcga ggtggaaacg ttcgcggtgg agtggacgct gccggtggcg
     1801 accggagtca tggccgggca ggtccatctg tgggggaacc ccgccgatcc ggggttgatt
     1861 ttcgtctcct acaattcgaa gcagggggtt tccgacagcc tcttcagcga cagttgggcc
     1921 tatgtgacgg cggttgatcg gaccggccgc gtgcggttca accgggtgat tgcggccgag
     1981 cacggcggca tcgcaattat cccggccgaa cagccgggcg agtactatct cgggcactcg
     2041 acccccctca cagagcccgc ggatacgctc gccttcttgc agccggtccg ccgggtgtcg
     2101 cgcatctcga ccgacggcgc cgtgctcgat tcttcgccgc ccctctctgc tcccgtgcgg
     2161 gctttgtggg tcccgcccgg cgccggcgac tcgggcgtgt gggttttgac ccaggacggc
     2221 acggtcagcc gtttcgatcg ccgcctccgc ctgatggccc gctccgcgcc ttcgcacctg
     2281 cgttccttcc agggaaccat cgacttgccg gggcggagcg aaccggcctg gctgttcggg
     2341 tcggaactcg gcgcggtcgt catgaccgcc gacctgaagc ctctcgcggt gctcccggcg
     2401 gtcggcgagt accgcgccct catcgcgttc gatccggagg ccgagcgcgc atcctatgtc
     2461 ttctccggcg ggggcggcgc cctgatcgcc gaatttgacc atcgcccgct ctggtcggtg
     2521 gcgagcaatt tcctctggcg ctaccacttg cacctctcgg cggtcgccat cgccctgctg
     2581 gtcacgctgg tgattgtcaa tcggcggcga ctccaggtgc agcggctgct gcgccagcgc
     2641 gaggccgact ttctcgcttt ctgccaggcg tcgccggatc tcgtgttccg gatcaatcgg
     2701 gacggcgtca tcactgattt tcatgcccgc gagcacgccg atctgctgtt gccgcccgag
     2761 gttttcctca accgcccgct ctccgaatcc ctgccgccgg cggtagcggg tccggccatg
     2821 gaggcgatcg ggcgcgccct cgacacgggc gccatggaga gcctcgagta cgacctggac
     2881 cagggcggcg agcgccggca ctgggaactg cgcatggccc cggtcgatga ctgccaggtg
     2941 ctcactgtga tccgggactg gaccgaccgg gcgcgggcgg aaaacgagtt gcgcgaatcc
     3001 gaggagcgct tccgcatgtt gttccacgga tcgcgcgacg cctatgtcat tgcggacgcc
     3061 gagaccggga aggtcatcga tctcaacgcc cgcgcggagg cgctgattgg ccggccccgc
     3121 gaccgcctga tcggccgcca tgtctcggag atggcgccgc cggatatccg cgccaatttc
     3181 ggggagcgct acaaacggca cctggaaatc gcgagcaccg gcaagtggat caacggagtc
     3241 gtctgcggcg aagacggccg gcgctgcccc gtcgaaataa gccacaccct gattgagatc
     3301 ggcggccgct cggttctgca cacgacgttg cgcgatgtca gcgagcgctt ggcgactgag
     3361 gcggcccttc gccgcagcga agaccgcctg gcggcgctcg tcgccaacat cccggccgca
     3421 atctaccgct gcctccccgg cgaggcgcgg ctgatggaat ttgtcagcaa cgagatcacc
     3481 accgtcaccg gttacgagag cgcggttttt gagccgggcg gcggcacccc ctacgcgagc
     3541 atcgtgcatc gggacgaccg gtcgcgcgtg agcgaggtga tcgccgccgc ggtcgccggc
     3601 cgccggccgt tctccgcgga ataccgcatt gtccgcgccg acggcgaggt gcgctgggtc
     3661 cacgagcggg gccaggccgt cgccggcccc gatggaacgg tcgcctttct cgacggcgcg
     3721 atctacgaca tcaccgagca gcgggccgct atcgacgccc tccacgagag cgaagagaag
     3781 atgcgggcgc agtacaaggg cattcccgtg cccacgtaca cctggcagcg ggcgggcgac
     3841 gacttcgtgc tggccgacta caacgacgcg gcggtcgcga tcacgggcgg cacgatcgcc
     3901 ttgaaagccg gcatccggct ctccctgctg tacgccgacc ggccggacct gcgcgccgac
     3961 atcgcccgat gcttcgacga acagacgacc attcggcgag aaatggacta cacctttcag
     4021 tccctcgacc gcaccgtttc gctcatcgtc cactacgtct acgtgccgcc cgacctcgtg
     4081 atggtgcaca ccgaagatgt caccgatcgg gtgcgctccc ggcagcagct ggcgacccgc
     4141 ctgcgctacg aggaaggcct ggtggggtgt tcgctgacgc tgcttcagac gcccgtatcg
     4201 gcgggggcgc tgaacgatgc gctctaccac ctgctgacgg cgctcgactc ctgccgcgtg
     4261 tacgtctacc agaacttcac ggatcccgag ctcggcctgt gcgcgcggga ggtctacgaa
     4321 gtgtgccggc cgggtgtgag accgacgctc ggccggccgg agcttcagcg ccgcccctac
     4381 gcgccgcagt ttgcgtccat gcgggaccgg ctggctgccg gtcgcccggt cggcggcgta
     4441 gtggccgaga tgagcgagcc cgtgcgctcc gtcaacgccc cctgcggcgt cctctccgtc
     4501 ctcaccctgc ccgtctttgt ccgcggccag tggtacggct ttatcggttt cgacgacacc
     4561 cgctcggccc ggcgatggag cgaggaagac gtccggctgc tgcgggcggc ggccgcgatg
     4621 gtcgggtcgt acctggagat gatcgaggcc aaccgggaac tggcggccga gcgggatttc
     4681 acgcggatgg tcctgcagac cgccaacagc ctgatctgct gcctggacga gcgggggtgc
     4741 atcacggtgt tcaacgacga gctcgaacgg gtcacgggct accggcgcga ggaggtgctc
     4801 gggcggtcct ggcacgaact ctttctgccg gagcactttc accaccccgg cctcgtccgt
     4861 ttcggccagt gggtccgcga ccaccccgcc gaccgctacg agcgggcgct cctgacccgc
     4921 accggcgagg agcgcatcat cctgtggtcg aactcgatgc tcgaggaccc gcacaccggc
     4981 gcggtcaccg ccatcgccat cggccatgac atcaccgacc gcaagcacat ggaaacagcc
     5041 gtgcgcgaga gcgaggaaaa ataccgcgcc gtcatggagc agagcgccga cttcatctac
     5101 ctcgcccagg ccgactcggg gcgcatcatc gaggccaacc gctcgcttcg cgagatgctc
     5161 ggctattcag tcgaagaaat gtgcggaatc tcgctctttg acttcgtcga ccacgattcc
     5221 ggcgaggttc gggaaggctt ccggaaggtt ctcgccgggg gactgccctt cctccgcgag
     5281 cggtactacc gcacccgcga cgggcggcgc atcccggtcg aagtcggcgt caacgagatc
     5341 gtctacggcg gccacaaggt cctgtgcatc gtctcccgcg atatctccga gcgcaaagcc
     5401 gccgaggagt ccgtgcggca gcaggaagag aagtaccgtc tcctcgtcga gaacgtccgg
     5461 gcctcgatca ttctcgtcga tgccgagggc tacttcctgt tcgtcaacca gaatgccgcc
     5521 caggcagtcg gccgccgtcc ggaggaactc atcggccgga ccgagtggga tatcttcccc
     5581 gaagatctgg ccgaccggca gatgtccaac gtccggcggg tgatcgccga ggacaaggag
     5641 ttcatcagcg aggcgccggt ctacctcagg ggcgagtggc ggtggttcta caacaacgtg
     5701 cagccctacc gcgacctccg ggggcgcacg gtggccgccc tggtcattgc tcacgacatc
     5761 acgatggcga agcgggtgga agaggcgctg cgcgagagcg aggagcgctt ccgcgagctg
     5821 gccgatctct tgccgcagac cgtcttcgaa accgacgtcg tcggccgcct gacctactcc
     5881 aaccgccacg gctttgagtc caccgggtac acatcggccg acctggaggc cggcctgacc
     5941 gtcgaccagc tcttccttga aagcgaccgc gccgaagtcc gcgccaactt cgccaaacgt
     6001 ctggcagggg agaaccccca gcgctacgag tacacgcacc tgcgcaagga cggttccact
     6061 tttcccgtac tcatctactc ttcgcgcatc gtgcgcgacg ggctgccggc gggactgcgc
     6121 ggtgtggtgg tcgatatcac cgaacgcaag cgggccgagg aagaggtccg gcgggcgaac
     6181 cttctccgct accagcagat gcgcgaaatc gccggcggcg tctcccacga aatctacaac
     6241 tctctctatc cggcgatcgc caccctcgag aaattgcggg ggctgctggc cgcttccgac
     6301 acgaccgatc tcgcccgcac cgagcgtctc atgcagttgg ccgatcgcgc cgtccgccgc
     6361 gccatttccc tgacagaaac cgtcacccag tacgcccggc tggagtccca gcgccgggtg
     6421 gagcgggtcc ggctcgccgg cgtcattcag gagatcgtcg agcagaaccg ccacgccgcc
     6481 gaggaactgg cggcgaccat caccgccgac atccccgccg atctctttgt cacggtcggc
     6541 aaatcgtacc tctacagcct gttcaacaac ctcgtgatca atgccctcga cgcggtcgcc
     6601 gaagtaacgg aacgggcggt gtcgatcgcc gcccggccgg acgacggccg ggtgcgcatt
     6661 gtcgtccgcg acaccggacc ggggattccg ttcgagatcc agcagcgggc cttcgagccg
     6721 ttcttcacca ccaagctcga ccgtgggacc ggcctggggc tggcggtcgc caaacggatc
     6781 gtggagctgt atgatggtca gatcggtctt gaatccgcgc ttgacagagg aaccacattc
     6841 attatattgc tcccaggtgg cagtcacttg taagcagttg tctaccagtg tctaagagcg
     6901 gacagattct catcgtcgac gacgaccgca tggttctcga ggccatgctg gaatcgttca
     6961 ccgatgatta cgctgtcctc accgcgctct ccggccagga ggcgctcgag cggatcaaag
     7021 agcaccccga gatcgacacc atcatcctcg acatccgcat ggcccgcatg gacggcctga
     7081 aagccgccaa cctcatcaag gacatcgccc ccgaaatccc gatcatcttc cacaccgggt
     7141 ttgcgggaaa ttactcggag gaggatatcg accgcgacta ccaccccttc gacttcatcg
     7201 gcaagaacga accaccctcg cgtctccggc gggcggtcaa gaacgccgtg cagtaccacc
     7261 gtctgggcaa cacgggaagc gacttgatcg atgcggcgcg agagcgctac ggtatggtgg
     7321 ggaagtcgcg cgacatgcgc cagatctacc agcggatcga gcagatcggc ccgaccacgt
     7381 cgaaagtcat gatcctcggc cccaccggga gcggcaaaga gctggtcgcc cgcgccctcc
     7441 atgcccggag ccgccgcgcc gaccgggaga tgatggtcgt caactgcagc cagaaagacc
     7501 cgaccatggt cgagtccgaa ctcttcggcc acaccgaggg gtcgttcacc ggggcggtgg
     7561 ccaaccgcgt cgggtatttc gaagccgccg acgggagcac gctgttcctc gatgagatcg
     7621 gcgaactcga tcccgccacc caggcgaaaa tcctccgcgc tatcgagtat ggcgaggtca
     7681 ccccggtggg cacctgcgtg aagcgcagtg tcgacgtccg cgtcatctgc gccacccacc
     7741 tcgacctcga ggaacaggtg cgcagcggcg ctttccggat ggatctctac taccgcctca
     7801 aaggcgtgac gatccgcctg cccgcgctcc gggaacgccg ggaggacatt cccgagctcc
     7861 tcgactactt catcgagcgg acctgccgga tcaacggggt cggcctgaaa gtgttcgagc
     7921 cgtcggcccg cgccctcctc gtcgaattcg actggcccgg caatgtccgg cagctctccg
     7981 acacggtgga gtcgctcatt caactttcgc ccggttacta catcacctac cacgacgtgg
     8041 ccaaccacct cgggtacgag agcagcgccg cccccaacgg aaactccggc ctggccgaac
     8101 aggtatggga attcaagcgg atgaagatcg tgcaggcgct cgaccgggcg cgcgggaacg
     8161 tgatcgcggc cggtgaggag ctcaagatcg atccggccaa cctgcgcaag atgatcaagc
     8221 gccttaacat cgagtcgtga tcgtgggggg acggcgcggc gggaaatgaa aaagccggtg
     8281 gcgggcaagg ccgccgccgg cttcgtgttt gtgccgcgcg gtcagcaggc cgccggagcc
     8341 ggcccgccgc ggaagaggta gttgatcagc agggtcaggt ccgacacctt gatggcgccg
     8401 tcggcgttgg tgtcgccgtg cgggagacaa atcggcgcga ccccgccgcg gaagaggtag
     8461 ttgatcagac cggtgagatc cgacaccttc accgcgccgt cctcgttgaa atccccccgc
     8521 agcacgcagc agccgcccga cccgaagtcc tgccagaaac cctggttcac ccggtaattg
     8581 gccgacgatc cggcgccggc ggccgtctgg ccgaccgtcc cgctcagccg gtaattcgtc
     8641 gacgagccgt tgccgccgcc cgcggcgatg acctgccagt cgattcgctc gccggcggcc
     8701 gaggcggaca ccgcccccat cagcacgagg ccgacggctg cgaggaggac gcgtttgtac
     8761 atgcctgtaa cccttatgac gaggtgcgta tcttcttgcg tacgtcttga cgtcggcgac
     8821 tatccggtat gaacgaccct gccgagcagc ggcggccgac gggcgctttc tgctctcgtc
     8881 tcaagtaagg caatcgcgcc gaaaaagcaa gcaaaacctg tcattccggg ggcgggggag
     8941 aaggatcgcc ccgggttttg ggacgccgaa tctgcgttcg ggccgccccc cgcaacaaaa
     9001 gcccctgccc ctcgtctcaa gcggtaggtc tctggtcatg gaggacggtt tcgcgcaccc
     9061 cggacagccg gcgggccggg gggcatcagc ggagaatcct gccgccgccg gcccgctttt
     9121 ggccggtcgg tcgccggcct atccgtaatc gatgttgact tgggtgtcga atatgatata
     9181 ttgaacctgt acccccgatg atccgccgaa ccgccgtggg gtgctagctg gaaaggagtg
     9241 gcaatggggc atcgtgtacc cacgccccgg gagaagacct cctcaccact cgcagtatct
     9301 gtacacgcta acgtaaactt gtggccatac atgatcaacc gttaacggag agagaaatca
     9361 tgaaagcgcg caacgttctt tgcctcgcgg tgggcgtctg cctgctggcg ttggtcgggc
     9421 tggtgcaggc cgaaacggcc ccgccctcag ctgcgctcaa caccaagggc ctggcaatcg
     9481 gccaggttcg gactgtccat ttcggcggcc cgcagacgct gagcatcgcg ggcgagcaaa
     9541 tgaccttcac cgcgccggag cccgctcccg agcaggatcc ggattttgtg tacaccctga
     9601 cggacaagcg gcaggggggc gaaaacatcg ccaccgcctt tgtcatcccc ggtatgccct
     9661 tcaccgacac cgggaccacc gtcgggtacg ccgatgacta ctccgaggcc tgcgacggcg
     9721 tgaactacag ccacgtgggc gacgtcgtct tcgcctacgc cccgccggtc aagcagctgg
     9781 tcgacatcaa gctgtgcaag tccgactact acacgcggct gtgggtgtat cagaccgacg
     9841 agaccaacat gatcgcctgc aaccggttca ataccccggc cgtttgctct cccaacccgc
     9901 gctcggcgct catggatgtc cagatgctgc ccgggttcac ttactacatc gtggtcgacg
     9961 gcgaccacca ggtcactccg aacgagggcc agtacatcat tgagtgctcg gcgactctcg
    10021 tccccgactc ggtcgaccgc tggcccgccc tggccgacaa cgacaacggc ctgctcctgc
    10081 tcggttactc caacaacaac ggggtggact cgcagatgtt ctggcaggag tcgctcgatg
    10141 acgggctgac tttcgaaccg gccggctatt ggaccaaccc cggctggcag aagtacgcct
    10201 cggtcgacta ctgggggcag gattcgatcg cctacgggac gtttgtcccc agcccgctga
    10261 acaacaacgg cggttcgact tatctccacc gcttttacaa catcgccaat tcggccgggt
    10321 ggagtctgag ctactggaac tggacttcgt acggctggcg gaacatgcgg atggccgaca
    10381 tcgcctgcga tgacggcttc attttcagcc cgaaccccgg cgatcaccgc ttcggcgtca
    10441 tcgcaatggt gcacagcagc acgtactcca gcggcggcgt tgtagtcggc accgatgctc
    10501 cgcacctctt ctaccagacc gattcctcca actacgcgac gatcagctgg tacaacaacc
    10561 tcaacggctg ccgttcgacc acctgtgaca tcgaccgcgt gaccaagtac tcctacgccg
    10621 tctacgactg gctcgatacg agcaagacgc ccgaccagtg ggcgctgttc attcgcggcg
    10681 acgttttcgg caaccccgac gacaccctct tcagcggcgg cgtgacgtac agcctcgacc
    10741 cgggcgagca catcggctac ccggcggtcg cggccaacaa cggcacggtg ctcgtagcct
    10801 gcgagttcta caccgaagcc accccggccg accatgacat catcttcttc tattccgggg
    10861 cggccgacgg cgcctacgac aacatgctct cgggcgtggt ggtcgcgacg gcagacgacg
    10921 agcggtatcc ggagatccag cacgtgaccg gcaatacctt catcgtctcc tacatcgcga
    10981 acaaccagtt gtacatgacg gtcactgagg acggcggcac gacctggagc acgcccagcg
    11041 tgatcagcgg cgccgaccac gtggtcaacg aataccgcgc ggccgacatc gccgacttcg
    11101 ggaagaagat catctgggag taccagccga accatcccgc cgacaccacc atcatgctgc
    11161 actgggccga gaccggcgtc gtgcaggatg ccgacggtga cggcgttccg gatgaagacg
    11221 acaactgccc ggcgatcgcc aacccgctcc aggaggacgc cgatctcgac ggcatcggcg
    11281 acgtgtgcga cgcctgcacg gacaccgaca acgacggcta cggcaacccc ggttatccgg
    11341 ccaacacctg cgcgctggat aactgcccga acgtggcgaa cccgggccag caggatacca
    11401 acggcgacgg tatcggcgac gcctgctgct gcctcgacaa ccgcggcaac gtcgacggcg
    11461 atcccgacga tctcatcaag gtgggggacc tcacctacct ggttgagttc ctcttccgct
    11521 tcggcccggt gccgccctgc ccgaacgagg cggacattga cggcgacggc aatacgaagg
    11581 tggccgacct gacctacctg gtcgccttcc tcttccggtt cggtccggcg cccgcgacct
    11641 gtccgtaagg cggtcgttgc gggtacgaac ctgccgaata cgatcagccg cctccccctg
    11701 gggaggcggc ttcttttttg gccggtcgga cggcagcagg ggcggccgaa attgcttgcc
    11761 atatctcctc cggtagtgta tctcacgaag agaagccgcg gtgagcgcgg cgccctcgcg
    11821 ggagcgggca ggaggatgcc cgccgacgga tcgcgggcgc gggcgatcag gcggcgggga
    11881 gaagcacgaa gcgaaaaatg gcctctcccg gcccttcgcg ggcgttgcgc cggcattgtg
    11941 gtctccggtc ggccgcggcg gggcggccgg gcacgggacc ggtaagggaa acgagttcag
    12001 tatggctaag acggtgacca acagcgaact gcgctttgat gtcggcgcca atccgatccg
    12061 ctactgcggc atgcagaaag tctatccgtt cagtcgcgac cgggtggtcg aaatcaacct
    12121 gcaccaccag cacgttctgg agatgtacga tctcgagctg gtcggcggga cggcgatttg
    12181 cctggccgac cgcctcaacg agcgctccaa tctcggggcg ctgcgcaatc gcaaactgcg
    12241 cgaagcggcc gtggcctcgg cggccctctc ggccgtggcc gtcagtctgc acggacgcgg
    12301 gagcctcgcg gccctgccca aagacaagca gaccaaagag gtcgccaacg agctcaagcg
    12361 cgccaacgac cgcacctcgg cccaggtcat ggccgaggtc ctccagacga ccaccgaaac
    12421 cctctcgatc ggcgaggaag tcctcatcga gtcgaccatt accgaaggtg tccgcgccaa
    12481 accgggtaaa gaagtcggcg gcaacccgac catcgccgtc ggcgccctct tcggcaaaaa
    12541 ggagcaccgc gcccgctacg gcctcgacct gggcaaggat gtgacgttgc tctccatggg
    12601 caacgacgtg atcgacggga ccaccaagtc ggtcaagggt ctgcattcgt cgatgacctg
    12661 catgttcctc atcgagtcgc aggtgaagcg gcacctccct gatatctacg tgcagcggtg
    12721 gatgggcggg gcgcacttcg acgagttcaa cccgcgcgag gggacgctcc tggatgccgc
    12781 ccgcgtgatt gccgacagct acggctttgc cgatctcagc gaactcagcg ccttcttcat
    12841 cgaccgctcc cgccactacc cggcgtggga taccctcggc gcggccggga tcggcattcc
    12901 gtacgacgcc gacggcgacc tgttcccggc cctgctgctc ggactggagg ggatgcgctt
    12961 ccccaacggg cggcacatga attcgatgat cggggaaatc ggcgggtcgg ccgagtgggt
    13021 ggtcggcgcg ctgccgctcg tctggcgcgg cggccaggcc ctcggcatgc tcaccagcca
    13081 ctcctccctc tcccgccgcg acctctcgcc ggaagatctc tggaacagcc gcttccacta
    13141 caccgaggac gagctcatca tgatccagga tgcccgcttc cagcagaagc cgtatttctc
    13201 ggtgaaggac atcatcgaga acccccacgc cggcggcatc agcgccttcg gcgccatcac
    13261 cgacagcgtc tacctcccgt tcatgaaggg ggtgcagttc gatccggaca aggggaagat
    13321 atcggtgtgg acgctggtga tcaattcgct cgggctgatg gaagtgtggg agatgcactt
    13381 cgcctgccgt acctcgctcg cccacaccag ggctttgatc gcgtcgccca aggagcggct
    13441 ggccgggctg cgcgggaaag cgctcgaggc ggccatcggc aagatgctcg acgacccgcg
    13501 cgaggaacgg cgctaccgga tctttttcaa caacgagtac tacccggcgc tcgtgccgat
    13561 ccgcgagaaa gtggcgctct tggggcaggc cctcgaaggg ctgatcgccc gcggcgccct
    13621 caacgaccag gaccgcgaga ttatcgacct cacggcccgg ctcgcccccg agtggtgggt
    13681 cgagaacggc cgatagccgc gttcccgccc cgaccccaat gacgacgccc ctcggtcagt
    13741 gaccgagggg cgcatctatt tggtgcggat ggccggactc gaaccggcac gggtcgcccc
    13801 actaccccct caagatagcg tgtctaccaa tttcaccaca tccgcaagcg atcgcgatgt
    13861 caaagatcgg tcggagcgcc aacgcccgcg ccggcgcgtc ctgctactgc ccgtcgcccg
    13921 gcgccgtctg ctgcccctgg cccggctgcg tgccgggatt ggggttgacg ttgaggaggg
    13981 agtcggccga gggctgctgt tgttgcgtct gctgctgttg ctgcacagcc tgttcgcgcg
    14041 cgcgctgcgc ctcgcgggtg acgaccgact cagtcggacc ggccgtcgtg ctctggctgc
    14101 gcaggccggt gcggctcgac gacatgaagg ccagaccgat gctgttcacc atgaacagaa
    14161 tcgccagcac ggtcgtggcc ttgctcagaa acgacccggc gccgcggcca ccgaacaccg
    14221 ctccccccag cccgccgccg aacgccgtcc ccgccaaccc ttcgccctta ctggactgca
    14281 agagcacgac gatgatcagc gccacgcaga ccaggacgtg aaagactacc agtgcgatga
    14341 agaacaagaa acctacctcc gttatcgggc cgcgttaatg atcgccagaa aatcggccgc
    14401 cttcagcgac gcgcccccga ccagggcgcc gtcgatatcc ggctcggcca gcaagtcggc
    14461 ggcgttgtcc gctttcaccg atccgccata caaaatcggg atccggtcgg cgctcggttg
    14521 gccgtactgc gccgcgagac gatcccgaat aaaagcatgc acctcctgag ccatcgccgg
    14581 cgtggccgtt tttccggtcc cgatcgccca gaccggctcg tacgcgatca cgattcgttc
    14641 cagcgaaccg gactcgatct ccgcgcaggc gatgcgcacc tgagcgccga ccacttcctc
    14701 ggtcctgccg ttctgtcgct gttcgagttc ctcgccgaca catacgatcg ggatcaaccc
    14761 tttgcgcagc gccaacttca gtttggcatt caccgattgg tcggtctcgc ggtggtactg
    14821 ccgccgctca gaatggccca atataacgtg ggtggcgccg actgtcaaga gcatgtcggc
    14881 cgagatttcg cccgtgaaag cgccgttctc gtgcgccgac atgtcttggg cgcccacctt
    14941 tatcggactg ccgtgtaaca gtttagctgc caggtggagg gacgtgtagg gagggcagac
    15001 gagcgcctcc gcccgctggg gatcccagcg gccggtcaac aacgcggcca gcagtttctc
    15061 cgtctccgga atcgtgccgt tgcacttcca gttccccgcc agcagctttc ggcttctcat
    15121 cgccgcaccg cctcggcttt gccggcgttg gtgagcgccg ccacccccgg cagttccttc
    15181 ccctccagga actccagcga ggcgccgccg ccggtggaga tgtgggtgag gccgtcttcg
    15241 accccggcct ccttgaccgc cgaggcgctg tcgccgccgc cgaccacggt gatcgcgcct
    15301 tcaccggtgc gccgggcgag catccgcgcc accgcgaatg tcccctcggc gaacggccgg
    15361 tgttcgaaca cgcccatcgg tccgttccac aggatcgtgt tcgcctgcgc gagcttctcc
    15421 tcgaagagcc tgatcgtctg cggaccgata tccagcccct tcaggccgtc ggggatccgg
    15481 tcgatcggga ccgtcctggt ctcggccgtg tccgagatct cctgcgcgac cacggcgtca
    15541 accgggaaga cgagctcggc ccgcgacttc ttgatcttcc caaggatctc cttcgccagc
    15601 gcgactttgt cctcctccaa gagcgaatcg ccgatcgggt accccatcgc cttgctgaag
    15661 gtgaaggtca tgccgccgcc gatgaggagc aggtcgactt tggccatgag gttctcgatg
    15721 acgtcgatct tgccggagat tttggctccg ccgagaatgg ccgcaaacgg ccgtttaggg
    15781 ttctccagcg cctggccgag gtactgcagt tctttctcca tgagcaggcc ggcgatcgac
    15841 tggcggaaat agcgggtgac gccctcggtc gaggcgtggg cgcggtgggc gctgccgaac
    15901 gcatcgttca cgaaggtgtc ggccagcgaa gcgagtttct gcgcgaactc cggatcgttc
    15961 ttctcctcct tggcgtggaa ccgcaggttc tccaggagca ccacgtcgcc gtctttcatt
    16021 ttgttgacga cgctggcggc ctcgggtccg acgcagtctt cggcgaacgt cacctcccgg
    16081 ccgagcagtt cggccagccg ccgggcgacc ggccgaagcg acatgccggc caccggctgc
    16141 cctttgggac ggccgaggtg ggaggcgagg atggcgcggc cgccgtcgcc gatgatcttc
    16201 cggatcgtcg ggagcgcctc ccggatccgc cggtcgtcgg tgaccccgcc gttatcgagg
    16261 gggacgttga aatccacccg cacgaggact ttctgcgagc ggaaattgac ttcgtgtacg
    16321 gtgacttttc gcattgctgc tcctttccgt cttccccaat cacagcatca tgagcatcat
    16381 gtcgaccatg cggttggaga agccccactc attgtcgtac cagctgaaca ctttcgccag
    16441 ccgcccgtgc accatcgtca actggctgtc gaacaccgag ccgtgcgggt taccgatcac
    16501 gtcgaccgac acgatcgggt cctccatgta ctgcagcgcg cgccgcagag ccggcgattc
    16561 cgccgccttc ttcatggccg cgttgatctc ctgcaccgtg gcctcccgct ccagcgtcac
    16621 tgcgagatcc accagcgagg cgttcggggt ggggacgcgg atggcgcagc cgtccatttt
    16681 ccccttgagc gccgggatca cctcggcaat cgccttggcc gcgcccgtgg tcgtcggcac
    16741 catcgacagg gccgccgagc gcgcccgccg cagatctttg tgcggcgcat ccatgagccg
    16801 ctggtcggcc gtgtacgagt ggatcgtcgt catgaacccg ttgacgatcc cgaaactctc
    16861 cagcagaacc ttcgccaccg gggccaggca gttggtcgtg cacgatccga tcgaaatgac
    16921 gtggtgcttg tcgcggtcgt agaggtccga gttgatcccc gggataaagg tgccgtcgtg
    16981 gcccttggcc ggcgccgaga tgagcacctt gtgggcgccc gccgcgacgt gcttcatcgc
    17041 gtcgtccttc ttcgtgaact tccccgtgca ctcgagcacc acgtcgactt tgagctcctt
    17101 ccacggcaac tgggccgggt cgagcaccgc catgaccgga atcgtccgcc cgtcgacctt
    17161 caggttcgtc ccctcggcct tcacctcgcc ggggtagctc cggtggacgg aatcgtactt
    17221 gagcaggtgt ccgagtgtgg cggcatcggt gatatcgtta atcgcgacaa actcaatgct
    17281 ggtgtcctga gcggcccgaa acacgaggcg cccgatacgt ccgaatccgt tgatggcaac
    17341 tctcatggtc tcctcctgct atccgtttcg tcttttgccg gtctatatag atatttgatt
    17401 ccgatggaaa cggcaaggcc gtcatagtcc tcaatcgtca tgagcggctt gccgaaagga
    17461 atgggacaat acttggcgtt gatttccagg ccgatctgcg aggcgatcgg ccagtcgatg
    17521 ccgccgccga ccgcatagct gatcttcgcg gccgtctcct cgtcgatatt cgggtagtag
    17581 tagccctcgg ggaccgaggt gaactgcacc gagcgccgcc cgacatatag cccgccgccg
    17641 gccgtcagat acggctgcac gctggcgccg ccccccgcca gcggatacac cttcgcctgc
    17701 agcagcccgg cgtacaccag cacgttgccc acgttggttt ggttgccggc ggtgaacgtg
    17761 accgagccgc ggttgaggat cgccagcgaa agttcgccca tgagcgcggg tgtgagccgg
    17821 tgggcgtaaa acccctcgaa aaggaaactg gcgtcgtgaa aattggtctt gaacacttct
    17881 tcgccgccgc tggtctcccc gcgggccggc ggcatgtcgc cgaggttgaa ccacgggccc
    17941 agccgcacgc ccgcctggtg ggactggagg aagagctgcg aggtggtcgg ttcctcgccg
    18001 gccgcccgcg ccccgcccca ggccgcggcc gccagcaccg cgaacacg
//
LOCUS       DAOPOE010000065        17932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3756_length_17932_cov_5.181518, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000065 DAOPOE010000000
VERSION     DAOPOE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17932
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_17932_cov_5.181518"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKM94_09395"
     CDS             complement(<1..734)
                     /locus_tag="PKM94_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOD66892.1"
                     /translation="MAKFDNPEDIRIIVATDCGSTTTKAILIQYIDGEYRLTTRGEAP
                     TTVEAPFEDVTMGVLNAVAEVEELSGRKLLDDNGRFITPAQGDVGTDIYISTSSAGGG
                     LQMMVAGVVRSMTAESAERAALGAGAIVMDVIASNDKRLPHQQIERIRHLRPDMILLS
                     GGIDGGTTTHVVELAELISAADPRPRLGSSYKLPVIYAGNKDARDAIADTLAAEVDLK
                     TVDNLRPVLERENLGPAREEIHELFM"
     gene            1166..2413
                     /locus_tag="PKM94_09400"
     CDS             1166..2413
                     /locus_tag="PKM94_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD66893.1"
                     /translation="MFALVLTASAPAPAIAQALSDSSATDSVTVGADGAVTALRPAPP
                     GSIEAKDTPNDAGGSITVRWVLSPDDAAGGVTAYRVLRAEAQDGRPGEFTEIGGATAG
                     QDLYQDGNVSDDRQYFYKVVAANTVRLNGATLAVAEAESAPVGPVRSSAQWFNLGRIN
                     VFVGVLLLSGFIVYYIRRAQAGKRLFIRKIAGLEAVDEAVGRATEMGKKIFYVPGISD
                     MDNVQTIAAVTILSRVAEMSAQYEARLDVPVARSLVMVTSKEVVKEAYLKAGRPDSFH
                     EDQVHYLTDDQFGYAAGIDGMVTRERPATIFYMGQFFAESLVLAETGNSIGAIQIAGT
                     AEPAQLPFFVASCDYTLIGEELFAASAYLSREPRLLGSLKGQDIGKAIILIALIIGLL
                     LETFGNPLFGWDWHFSNLFSTGE"
     gene            2416..3063
                     /locus_tag="PKM94_09405"
     CDS             2416..3063
                     /locus_tag="PKM94_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66894.1"
                     /translation="MRREVPLVITFVVGLVFVVQYFIPHFPFNRLEGWFSEWFSIVGA
                     FAIWLGALNLMKLSIQSVARRRGDWWYSVVVIASFLLVAVIGFSAGSDYRDPGTSFDW
                     LYVNIYNPLSSTMYAILAFFVASASYRAFRARNLEATLLLVAAFLVMLGRVPVGDVLT
                     GFLPEGWRASAWADAIMTYPQTAGQRAIMIGIALGIVSTALRIILGIERAHLGGD"
     gene            3067..4077
                     /locus_tag="PKM94_09410"
     CDS             3067..4077
                     /locus_tag="PKM94_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66895.1"
                     /translation="MSTRFKEILVLTVLAAAAVIVYLGRGAGRLGAVSFSPLVSTIIM
                     VLIGLALLYILVRTIRGRLVDRRIVFLFVGLAVLLPLFMSLEQRMTISPEVRQVYDAV
                     GELPAGSRVLVSFDYDPASAPELQPMAEAFLYLAFQRDLKVVIMGLWPQGPQQANQAL
                     AKVLARPEIAAKHLEYGVDYLNFGYQVGNEFVIQRMGSSFQSMFPRDMSGRAYGDLPL
                     VRNVKNFSNVDYAFNLSAGYPGTQEWVLVAVGKFGLRMGAGNTAVQAPQMYPYWNTGQ
                     LIGLMGGMSGAAQFELLAGYEGKGTRYMLSQSFAHVVVIGFILIGNAAFFLGGRKPQR
                     KG"
     gene            4087..4755
                     /locus_tag="PKM94_09415"
     CDS             4087..4755
                     /locus_tag="PKM94_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66896.1"
                     /translation="MDHFALWLGAFLTLGIVSFLYRDNPWYKLCEAIFIGISAGYWFV
                     VSFWESLYRVLIVGLFPLQEVNQAGESITREPNYWLLVGGVLGLLMLMRLVPKVGWIS
                     RWPLAFIVGATSGLYMMIFFSSNIMEQVRGTIRPLFGPDFAASAGDIIGSTVVAVGTI
                     AGLVYFFFSKEHRGAFGVAARLGILFLMVTFGASFGYTVMSRMSLLIGRIDFLFGDWL
                     VLIR"
     gene            4839..5993
                     /locus_tag="PKM94_09420"
     CDS             4839..5993
                     /locus_tag="PKM94_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD66897.1"
                     /translation="MYGQVPDRAAGPGTGAPVAPPTSVLASDHEYDDGERLDLTWVPS
                     IDDRLGDGEVQGYIIYRQAAQGSFDSVASVPSQTNAYADEGLARGTAYRYIVAALSRG
                     DTAASAPTEAVAPDREWINFGRKWLFLLALVIGGAVVYFIQAAKAGRRLFIRPIAGLE
                     AVDEAIGRATEMGRPILYIPGIQDMDDVQTLAAITILGRVARQVADYDTKIRMPVSRS
                     LVMTAGRETIKAGYQAAGRPDGYSDEMVQYITDEQFGYVAAVDGIMVREKPATVFLLG
                     AFFAESLILAEAGNSVGAIQIAGTARPAQLPFFVAACDFTLIGEELFAASAYLSGEPK
                     ALGSLKGQDVGKGIALAAILVGVAAATLAGLGSETADAVWRWLTKVFSTI"
     gene            5990..6664
                     /locus_tag="PKM94_09425"
     CDS             5990..6664
                     /locus_tag="PKM94_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66898.1"
                     /translation="MRREVPLFITFFIGMLLIFAVFIPPIENLGEDFTVFFDIIAVFA
                     FFLGGGNLLRVHVTKLSRRQTDWGYSIVTLGGFLGMLVIGLFKLGNPGGIAAAPTESG
                     SLFQLIFDYVLVPLGATMYSLLAFFVASASYRAFRAKNREATILLVAATIILIGRTPF
                     GMYLTSWMPESLSVLEIPNLAMWIMNAPNLAGQRAIMIGIGLGVVAMSLRIILGIERS
                     YLGEER"
     gene            6661..7626
                     /locus_tag="PKM94_09430"
     CDS             6661..7626
                     /locus_tag="PKM94_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66899.1"
                     /translation="MTVFPWLTAPLVAAADIGRAVSLLVIGGGLLWLFVRSFQGKEVG
                     RRVIFIFIFLAVAAPLLFPITFTEKATPVVKRLYDKLESIPAGAHILISFDFDPAMAP
                     EVQPMANAITRHAMLRGHKVIFMSLWATGQALMTQTLENVIAREFPDKRPGEDYVVLG
                     YKAGNEGVLNVIVTDLKKMFPTDIDNRPYSDIPVLEGVKSVRDVELVISIGGGKPGVK
                     EWILFVGDPGGVPIAGGVAAVVAPEIYPYYPKQIVGILGGVKGAAEYESELAHNYRDR
                     LAGMDTPGLRMMGPQTLSHMVIIAFILIGNFSFFMARRKSRGQGA"
     gene            7623..8435
                     /locus_tag="PKM94_09435"
     CDS             7623..8435
                     /locus_tag="PKM94_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66900.1"
                     /translation="MRRPAVLVLAAVVLLCAVSWAVKATGLRGAEEHYIRQGLLVTVA
                     AFLTLAILSFLYKDNPFYKFAEHLFVGVSAAFWMSMGFWSTIVGNLVPRVSETLSRFF
                     EVPYDPEARNLFYLLPVVLGILLLLRLSRKAGWISRWPLAFIVGTTAGFNFVRYLRSD
                     FIEQVSSTFVPLVVDWQGAGHFLSNLSLSATSQLVLMAANWVVFLGVVCGLIYFFFSK
                     EHRGAFGAASRVGIWILMITFGASFGYTVMGRISLLVGRVTFLFDQWLGLIG"
     gene            8467..9867
                     /locus_tag="PKM94_09440"
     CDS             8467..9867
                     /locus_tag="PKM94_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66901.1"
                     /translation="MKLRWLLALGLAVVLAGPAFGQEAAPADTALASPPAAAIAAPAP
                     PVNVRAVDKPNDHGHAVVLTWDLSADDGGGAGSVLGYEMYRWVPYLVDTLATIRTAAE
                     AVRTQIAEGGGGLGHRRDSLREAYLETVDRLRAAYAGYPAGGEFRTWDGAVAPAGTRT
                     FTHTGNKDEWDDAFVPDHTDFYYRVDAVTADPLVRASSAVVGPVQSAGQWFNTGKVAV
                     LIAVLLFGALIVILVDRARRGADLYVRPLAGIEAVDDAIGRATEMGRPILYVLGLGTA
                     ADVATIASFTVLGRVAKRVAEYQTSLNVPTYDPIVMSVAQEVVKSAYLDAGRPDDYKE
                     DIVHFVTQSQFAYVAAVNGIMMRERPATNVYMGKFYAESLILAETGALAGSIQISGTD
                     EIAQIPFFIVACDYTLIGEELYAASAYLGREPVLLGSLKAQDYMKAAVIVLAIIGLFA
                     TNLGWAAFRTWFNVGN"
     gene            9884..10714
                     /locus_tag="PKM94_09445"
     CDS             9884..10714
                     /locus_tag="PKM94_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66902.1"
                     /translation="MKRQIPFLLVFLFALFQVVQYFVPHESFEWLYEFFLDWIYIIGI
                     FALALGIWSLFRVSVDRIRSKRPFWGYNYLTLIGLFLMISFGFVGKEAGAQSWGTYWL
                     WLILFTVLLLALIQIGATGDAGRKKLYALVALVLVVVEFLLPALDKNWGFFFYTQEGL
                     TNPMFRHFFDYVMMPILATMFALLAFFIASAAYRAFRARNLMATLLLLAALIVMVRFN
                     PYLPFAAETGFYSKLSNWLMNVPNQAAQRAILIGIGLGIVSTSIKVMLGIERGYMGRG
                     "
     gene            10725..11603
                     /locus_tag="PKM94_09450"
     CDS             10725..11603
                     /locus_tag="PKM94_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66903.1"
                     /translation="MSIFEKLMKLDRRWVFLFLVVVCVVAYAMPFTVPILVEPEVRSI
                     FDFVEQLKPGAIIFIAIDYDPNSLAELHPMAYAMIEQAWRQKKRIIFTALSQNGPGMA
                     DQLIRDVSDSLKVEKEYNGVVYPPREIVNGIDYAFLGYKPYYGLVILGMGQDFRIPFP
                     SDYYGTPLDSLPMMQGVINYGQVDLVVDLSSGNITDAWINYGQGRFGFPLALGLTGVS
                     TAQYYPYLGSGQVLGIMGGLLGAAQYEYLADNPGRAMDGMKVQLWAHIVIILFIVMGN
                     VGFFASRRRQKREGGQ"
     gene            11605..12444
                     /locus_tag="PKM94_09455"
     CDS             11605..12444
                     /locus_tag="PKM94_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66904.1"
                     /translation="MDFGTFLWFTGGVFLTLATFSFLYKDNPFYKVAEHIVVGVSAGY
                     FVIILWHNGLVPKLFQPLSDGSVYLAFLDSTQWYRIIPALLGVMMWTRFSKNYQWVSR
                     WPMSMYIGIAVGVSIPLDLGNRVIRQLQATMYDFKWDTWGDFIGSSNLFYVLDPFSGL
                     SQVIIAVGSIAGLTYFFFSKAHTGVFGRVATFGIWILMIGFGASFGFTVMARISLFIN
                     RVQDVDSWRKVGWNAGETPIEGYSAWYQLVGWLLVAVVVAYIVNEIRLYLKSRNQVAG
                     PQV"
     gene            13111..13323
                     /locus_tag="PKM94_09460"
     CDS             13111..13323
                     /locus_tag="PKM94_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66905.1"
                     /translation="MHKEKAKEYQRQYNLKHKKKARGGRGKANSLCPREVVRTTFNSS
                     DIMHAPAEKAARILDKIIKGERLFTM"
     gene            13467..16388
                     /gene="ppdK"
                     /locus_tag="PKM94_09465"
     CDS             13467..16388
                     /gene="ppdK"
                     /locus_tag="PKM94_09465"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOD66906.1"
                     /translation="MPAKKAAKKTVKQTAKQASKKDAGRKTARPTPSEKTPKKIAPPK
                     DAGGLKKTAHKPAPAAKRPAGKNVAVSAKIAEAVAKGKFYYYFGDGKADGSADMKALL
                     GGKGANLAEMSRAGVPVPPGFTITTEVCNLYYDNNLQVPGPIDADLEKHVGMIEKSVG
                     KEFGNPADPLLVSVRSGAKFSMPGMMDTVLNLGLNAETVEGLAKKTGSQRFALDVYRR
                     FIAMFGNVVLGIDKSEFEQVIDKKKKERRIKQDSSLQEGDLRDIIKKFKHICRRKSGE
                     EFPEDPWQQLRMARDAVFRSWNNPRAISYRRMHDIPSDLGTAVNIQAMVFGNMGNNSA
                     TGVGFTRNPATGTKEFYGEYLVNAQGEDVVAGIRTPQPIVQLKEEMPQAYKHLVEITT
                     RLEKHYRDVQDFEFTIEEGKLYMLQTRNGKRTVQAAIKIAVDMVKEKLITKEEALERV
                     EPSSLDQLLHPRLDPRASYEVLAKGLPASPGAASGKVYFTAEDVVRHGEKTPAILVRE
                     ETNPDDIEGMNVAAGILTARGGMTSHAAVVARGMGKCCVSGAEAIRVNPAKKQFQVGK
                     LAIKEGEIITLNGTTGEVILGEVPTIEPELSGEFSEFMLWADEIRRLKVRTNADTPRD
                     AAQAIKFGAEGIGLCRTEHMFFAEDRIPIVQEMILAETPEERQAALDKLVRFQKKDFK
                     EIFDAMQGKPVTVRTLDPPLHEFLPNRDEVKAKIAKLEPKDEEYDEKLARLERVIARI
                     NELAETNPMLGFRGCRLGILFPEITEMQVRAIMEAACELARQKKAVLPEIMIPLVGHV
                     NELRNQKEVVLRVANEVVNKYKVKDLTYLVGTMIEVPRAALTADQIAAEADFFSFGTN
                     DLTQMTMGFSRDDAGRFLRHYVDSGILPKDPFVSLDQQGVGQLVRLGVERGRRTKPDL
                     KIGICGEHGGDPASIHFCNEVGMNYVSCSPFRVPIARLAAAQATLQEQKGKGKGRTGT
                     E"
     gene            16493..>17932
                     /locus_tag="PKM94_09470"
     CDS             16493..>17932
                     /locus_tag="PKM94_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD66907.1"
                     /translation="MKRALDAIFRPKSVAVIGASTRPGTIGRETLHNILVAEFNGKVF
                     PVNPKAPVIHSIKAYSTILDVPDSVDLAIVIVPKEFVAEVARQCGEKGVKGLVVISAG
                     FSETGREGKKREMELVRVVQDYGMRMVGPNCFGIVNTSPEVNLNTTFGKVFPKRGRVG
                     FITQSGAMGEAIMNHARELGIGFSLVASIGNKADISSNDILEYFRDDPDTDIILMYLE
                     NFGNPRHFTLIARELSRTKPIVAVKSGRTTLGAKAASSHTGALAGLDVGVDALFDQTG
                     VMRVDTVEELFDVAKALSMQPVPQGNRVAVVTNAGGPGILATDALINNGMEMPPLSPE
                     TVKRLRQFSSEDASFANPMDMVAGAGPEQFRRTLGAIITDKRYDSIIPIFVPPVTIDE
                     LEVARSIQEAVAGTKKTVLACFMGASRNSAGIEHLRSHGIPVYIYPEAIAKTLATVSH
                     YRRRMNAPRGKVRTFEVDPDRVQAVIERAR"
BASE COUNT         3062 a         5487 c         6002 g         3381 t
ORIGIN      
        1 tccatgaaca actcatggat ttcctcgcgg gcgggaccga ggttctcgcg ctcgaggacg
       61 gggcggaggt tgtcgaccgt cttgaggtcg acctccgccg cgagcgtgtc ggcgatggcg
      121 tcgcgggcgt ctttgttgcc ggcgtagatt acgggtagct tgtaggagga gccgagccgg
      181 ggccggggat cggcggcgga gatgagctcg gccagttcca cgacgtgggt cgtcgtgccc
      241 ccgtcgatgc cgcccgagag caggatcatg tcggggcgga ggtggcggat gcgctcgatc
      301 tgctggtggg gaaggcgttt gtcgttggac gcgattacgt ccatgacgat tgcgcccgcg
      361 cccagagccg cgcgctcggc cgactcggcg gtcatggacc gcaccacgcc cgccaccatc
      421 atctgcagcc cgccgccggc cgaggaggtg gagatgtaga tgtcggtgcc gacgtccccc
      481 tgggccggtg tgatgaagcg gccgttatcg tccagcagct tgcgccccga gagctcctcc
      541 acttcggcca cggcgttgag aacccccatg gtgacgtcct cgaagggggc ttcgaccgta
      601 gtcggggcct caccgcgggt ggtgagacga tactccccgt cgatgtactg aatgaggatt
      661 gctttcgtag tggtggatcc gcagtcggtg gcaacgatga ttctaatgtc ctcgggattg
      721 tcaaatttcg ccatcgagag tacctatctt tctcgccaat atgtcccttc ggttcttgcg
      781 gacacgccgc cggacgggtg cgaaagagca caccgaggcc cggcccgtgt caagatcgtt
      841 agtataacac ggcggcgacg gggatgtaaa gagcgatttt tgaaggcgtc ggtggggtgt
      901 gcggagagcg cccgggattg cgcgagggta cttttcgctt gactccgcct gccggccggg
      961 ctagattcaa cgctaattgt gtgccccagc gccggcgcgc tgcccggatg ccggcggcag
     1021 ccggttcaac cgttcgaccg ggtcgtgtgc ccgcggggcc tcgtccgcgg gtcgatttgc
     1081 accgtcagcc cgaagggagc caagattgcc cgttcgcccc acacgccctc tctggatctt
     1141 tcctccggtt ttccctctcg ctctggtgtt tgctctggtc ctgacggcct ccgccccggc
     1201 gccggccatc gcccaagcgc tttccgactc ctcggcgacg gattccgtca cggtcggggc
     1261 cgatggggcg gtgaccgcgc tgcggccggc cccgccgggc agtatcgagg cgaaagacac
     1321 gccgaatgac gcgggcgggt cgatcacggt ccgctgggtg ctgtcgcccg acgatgcggc
     1381 gggcggggtg acggcctacc gtgtgctgcg ggccgaggcc caggacggga gaccggggga
     1441 gtttaccgaa atcggcggcg cgacggccgg gcaggatctg taccaggacg gcaatgtgag
     1501 cgatgaccgg cagtatttct acaaggtcgt cgcggctaac acggtccggc tcaacggggc
     1561 gacgctggcg gtggcggagg ccgagtcggc gccggtcggg ccggtgcggt cgtcggccca
     1621 gtggttcaat ctcggccgca tcaacgtctt tgtcggcgtg ctgctcctgt cgggcttcat
     1681 cgtctactac atccggcgcg cgcaggccgg caagcggctc ttcattcgga aaatcgccgg
     1741 gctggaggcg gtcgacgagg ccgtcggccg ggccaccgag atgggcaaaa agatattcta
     1801 cgtcccgggc atctccgaca tggacaatgt ccagacgatc gccgcggtga ccatcctcag
     1861 ccgggtggcc gagatgtcgg cccagtacga agcgcggctg gacgtgccgg tggcccgctc
     1921 gctggtgatg gtgacgagca aggaggtggt caaggaggcc tatctgaaag cgggccgccc
     1981 ggactccttc cacgaggatc aggtgcacta cctgaccgac gaccaattcg gctacgccgc
     2041 ggggatcgac ggcatggtga cgcgcgagcg gccggcgacc attttctaca tggggcagtt
     2101 cttcgccgaa tcgctggtcc tcgccgagac cggcaattcg atcggcgcga tccagattgc
     2161 gggcacggcc gaaccggccc agttaccctt cttcgtcgcc tcctgcgact ataccctgat
     2221 cggggaagag ttgttcgccg cctcggccta cctctcgcgc gagccccggc tgctgggatc
     2281 gctcaagggg caggatatcg gcaaggccat catcctcatc gccctcatca tcggcctcct
     2341 cctcgagacc ttcggcaacc cgctcttcgg gtgggactgg catttctcca acctgttcag
     2401 caccggggag tgatcatgcg ccgggaagta ccgctcgtca tcaccttcgt tgtcggcctc
     2461 gtcttcgtcg tccagtactt catcccccac tttcccttca accggctgga ggggtggttc
     2521 tcggagtggt tttccattgt cggggctttt gcgatctggc tgggggcgct caacctgatg
     2581 aagctgtcga tccagtcggt ggcgcggcga cggggcgact ggtggtactc ggttgtggtc
     2641 attgcctcgt tcctgctcgt cgccgtcatc ggcttctccg ccggctccga ctaccgcgac
     2701 ccggggacgt cgttcgactg gctctacgta aacatctaca acccgctctc gtcgacgatg
     2761 tacgctatcc tggcgttctt cgtcgcctcg gcctcgtacc gggcgtttcg cgcccgcaat
     2821 ctcgaagcga ccctcctgct ggtcgcggct ttcctggtca tgctcgggcg ggtgccggtg
     2881 ggggatgtgc tcaccggttt tctgccggag ggctggcggg cgtcggcctg ggccgacgcc
     2941 attatgacgt acccgcagac ggcgggtcag cgggccatca tgatcggcat cgccctcgga
     3001 atcgtctcca ccgcgctccg gatcatcctc gggatcgagc gggcgcacct ggggggagac
     3061 tgatctatga gcacgcgttt caaggagatt ctcgtgctga cggtgctggc ggctgcggcg
     3121 gtcatcgtgt acctcggccg gggcgccggg cggctggggg cggtgtcgtt ttcgcctctc
     3181 gtgtcgacca tcattatggt tctgatcggg ctggccctcc tctacatcct cgtccggaca
     3241 atccgcggcc gactggtcga ccggcgcatt gttttcttgt ttgtcggcct ggccgtgctg
     3301 ctgccgctgt tcatgtcgct cgagcagcgg atgacgatct cgccggaggt gcggcaggtg
     3361 tacgatgcgg taggggagct gccggcggga tcgcgggtgc tggtgtcgtt cgactacgat
     3421 ccggccagcg cgccggagct gcagccgatg gccgaggcct ttctctacct cgccttccag
     3481 cgggatctca aggtcgtgat catgggcctc tggccgcagg ggcctcagca ggcgaaccag
     3541 gcgctcgcga aggtgctcgc gaggccggag atcgcggcca aacacctcga gtacggcgtc
     3601 gactatctga attttggcta ccaggtgggc aatgaattcg tcatccagcg catgggttcg
     3661 agcttccagt ccatgttccc gcgcgatatg tcggggcggg cctacggcga ccttccgctc
     3721 gtgcgcaacg tgaagaactt ctcgaatgtc gactacgcct tcaatctctc ggccggctac
     3781 cccggcaccc aggagtgggt gctcgtggcg gtggggaaat tcggcttgcg gatgggtgcg
     3841 ggcaacaccg ccgtgcaggc gccgcaaatg tacccgtact ggaataccgg gcagttgatc
     3901 gggctcatgg gcggcatgag cggcgccgcg cagtttgagc tgctcgccgg ctacgagggc
     3961 aaaggcaccc gctacatgct ctcccagtcg ttcgcccacg tcgtcgtgat cggcttcatt
     4021 ctcatcggca acgccgcctt cttcctcggc gggcggaaac cgcaacggaa aggttgagcg
     4081 ggcagcatgg atcacttcgc tctctggctc ggcgcgtttc tcactctcgg gatcgtctcc
     4141 ttcctctacc gggacaaccc gtggtacaag ctctgcgagg cgatcttcat cggtatttcc
     4201 gccggttact ggttcgtggt ctccttctgg gagagcctct accgggtgct gattgtgggg
     4261 ctgttcccgc ttcaggaagt caaccaggcg ggcgaatcga tcacccgcga gccgaactac
     4321 tggttgctcg tggggggcgt cctgggcctg ctcatgctca tgcggctggt cccgaaggtg
     4381 ggctggattt cccgctggcc gctggccttc atcgtcggcg ccacctccgg cctctatatg
     4441 atgatcttct tctcctcgaa catcatggag caggtgcgtg gcactatccg gcccctcttc
     4501 gggcccgact tcgccgcgtc ggccggcgac atcatcggca gcaccgtggt tgcggtcggg
     4561 acgatcgccg ggctcgtgta cttctttttc agcaaggaac accggggcgc gttcggagtc
     4621 gccgcgcgcc tgggaattct gtttctcatg gtcacgttcg gcgcctcctt cggctatacg
     4681 gtgatgagcc gtatgtcgct gctgatcgga cggatcgact tcctcttcgg agactggttg
     4741 gtgttgatac gctagtgcca ctcgggaaca cgacatcagc accgcacggt cgctcctgcc
     4801 tgttcttgct cgccctgggc ctcatagcgg ccgggtccgt gtacggacaa gtacccgatc
     4861 gtgcggccgg gccgggcaca ggcgcacccg tcgcgccccc cacgtcggtg ctggcgagcg
     4921 atcacgagta cgacgatggc gagcggctgg atctcacctg ggtgccgtcg atcgacgacc
     4981 ggctggggga cggcgaggtg caggggtaca tcatttaccg ccaggcggcc cagggttcgt
     5041 tcgactccgt cgcgtctgtc ccgtcgcaga cgaacgccta cgccgatgag gggctggcac
     5101 gcgggaccgc gtaccgatat atcgtcgcgg ccctgtcgcg cggcgacacg gcggcctcgg
     5161 cgccgaccga ggccgtggcg cccgaccggg agtggatcaa cttcgggcgc aagtggctgt
     5221 tcctgctggc cctcgtgatc ggcggcgcgg tcgtgtattt cattcaggcg gccaaagccg
     5281 gccgccggct gttcatccgg ccgatcgcgg ggttggaagc ggtcgacgag gcgatcggcc
     5341 gggcgaccga aatggggcgg cccatcctct acattcccgg tatccaggac atggacgacg
     5401 tgcagacgct ggcggcaatc accattctcg gccgggtggc ccgccaggtg gccgactacg
     5461 acaccaagat ccggatgccg gtctcgcggt cgctggtgat gacggccggg cgcgagacca
     5521 tcaaagccgg ctaccaggca gccggccgtc cggacggcta cagcgacgag atggtgcagt
     5581 atatcaccga cgagcagttc gggtacgttg cggcggtgga cggcatcatg gtgcgggaga
     5641 aaccggcgac cgtgtttctc ctcggggcgt tctttgccga gtcgctcatc ctggcggagg
     5701 cgggcaactc ggtcggggcg atccagatcg ccgggacggc ccgccctgcg caactgccct
     5761 tctttgtcgc cgcctgcgat ttcaccctca tcggcgagga gctgttcgcc gcctccgcct
     5821 acctgtccgg ggagccgaaa gcgttgggat cgctcaaggg gcaggacgtg gggaaaggga
     5881 ttgcgctggc cgcgattttg gtcggcgtgg ccgcggccac gctcgccggt ctgggaagtg
     5941 aaaccgccga cgccgtctgg cgctggctca cgaaggtgtt ttcgacgata tgagacgcga
     6001 agtaccgctg ttcatcacgt tctttatcgg gatgctgctc attttcgccg tgttcatccc
     6061 gccgattgag aacctcggcg aggacttcac ggtcttcttc gatatcatcg ccgtgttcgc
     6121 gttctttctg ggcggcggga acctgctgcg cgtgcacgtg acgaagctgt cgcgccgcca
     6181 gaccgactgg ggctattcga tcgtcaccct gggcggattc ctcgggatgc tggtgatcgg
     6241 gctgttcaag ctcgggaacc cgggaggaat cgccgccgcg ccgaccgaat ccgggtcgct
     6301 gtttcagctc attttcgact atgtcctcgt gccgctgggg gcgacgatgt actcgctttt
     6361 ggctttcttt gtcgcctcgg cctcgtaccg ggcgttccgg gcgaagaacc gcgaggcgac
     6421 gatactgctg gtcgcggcga cgatcatcct gatcgggcgg acgcccttcg ggatgtacct
     6481 gacgtcgtgg atgcccgagt cgctgtcggt gctcgagatc cccaacctgg ccatgtggat
     6541 catgaacgcg ccgaatctcg cgggacagcg ggcgatcatg atcgggatcg ggctgggcgt
     6601 ggtggccatg tcgctgcgca tcatcctcgg gattgaacgc tcgtacctcg gggaggagcg
     6661 atgacggtgt ttccgtggct gacggcgccc ctggtggcgg cggccgatat cggccgggcc
     6721 gtgtcgctgc tggtgatcgg cggcgggctc ctctggctct ttgtccgcag ctttcagggg
     6781 aaagaggtcg ggcggcgcgt gattttcatc tttatcttcc tcgccgtcgc cgccccgctt
     6841 ctgtttccga ttacgttcac ggagaaagcg acgccggtgg tgaagcggct gtacgacaag
     6901 ttggagagca tccccgcggg cgcgcatatc ctgatctcgt tcgattttga cccggccatg
     6961 gcgcccgaag tgcagccgat ggcgaacgcg atcacgcgcc acgcgatgct gcgcggacac
     7021 aaagtgatct tcatgtcgct ctgggccacc gggcaggcgc tcatgacgca gactctcgag
     7081 aacgtcatcg cgcgcgagtt ccccgacaag cggcccgggg aggactacgt cgtgctcggt
     7141 tacaaagcgg gcaacgaggg cgtgctcaac gtgatcgtca ccgacctgaa gaagatgttc
     7201 ccgaccgaca tcgacaaccg gccgtacagc gacatccccg tcctcgaggg ggtcaaatcg
     7261 gtgcgggacg tcgaactggt catttccatc ggcggcggca agcccggggt gaaggagtgg
     7321 atcctgtttg tcggcgaccc gggcggggtg ccgatcgcgg gcggggtcgc ggccgtggtg
     7381 gcgccggaga tttacccgta ctatcccaag cagattgtcg gcattctcgg cggcgtcaaa
     7441 ggggcggccg agtatgagtc ggaactggcg cacaactacc gcgaccggct ggcggggatg
     7501 gacacgccgg ggctgcgcat gatggggccg cagacgctct cgcacatggt catcatcgcc
     7561 ttcattctga tcggcaactt ctcattcttc atggcgcggc ggaagagccg ggggcagggc
     7621 gcatgaggcg gccggcggtt ctcgtgctcg cggcggtcgt gctgctctgc gccgtcagtt
     7681 gggcggtcaa ggcgaccgga ctgcgcggcg ccgaggagca ctacatccgg cagggactgc
     7741 tcgtcaccgt ggctgctttc ctcacgctgg cgatcctgtc gttcctgtac aaggacaacc
     7801 cgttctacaa gttcgccgag cacctcttcg tcggggtctc ggcggccttc tggatgtcga
     7861 tgggcttctg gtcgacgatc gtggggaatc tcgtgccccg cgtgtcggag acgctgtcgc
     7921 ggttcttcga agtgccctat gacccggagg cccgcaacct cttctacctg ctgccggtgg
     7981 tgctgggcat tctcctgctg ctccggctgt cgcgcaaagc cggctggatc tcgcgctggc
     8041 cgctggcgtt cattgtcggc acgaccgcgg ggttcaattt tgtccggtac ctccgctccg
     8101 atttcatcga gcaggtgtcc tcgacgttcg tgccgctggt ggtcgactgg cagggggcgg
     8161 gccacttcct cagcaatctc tcgctctccg cgaccagcca gctggtgttg atggcggcca
     8221 actgggtggt cttcctgggg gtcgtctgcg gcctgatcta ctttttcttc agcaaggagc
     8281 accggggggc gttcggggcc gcgtcgcggg tgggaatctg gattctcatg attacgttcg
     8341 gggcgtcgtt tggttatact gtgatgggcc gaatatcgct gctggtcggc cgggtgacgt
     8401 ttctgttcga ccagtggctc ggattgattg gatgacacaa gaggcactct caacgaggag
     8461 agaggtatga agcttagatg gctattggcg ctgggactgg cggttgtcct tgcgggcccg
     8521 gcgttcgggc aggaggccgc gccggccgat acggcgcttg ccagcccgcc cgccgccgcg
     8581 attgcggcgc cggcgccgcc ggtcaacgtg cgggccgtcg acaagccgaa cgatcacggc
     8641 cacgcggtcg tgctcacgtg ggacttgtcg gctgacgacg gcggtggggc cggctcagtg
     8701 ctgggctacg aaatgtatcg atgggtgccc tatctcgtcg acacactggc gaccatccgc
     8761 acggccgcgg aggcggtgcg cacgcagatc gccgagggcg gcgggggcct gggccaccgg
     8821 cgcgattcgc tccgcgaggc gtatctcgaa accgtggacc ggctccgggc ggcctacgcg
     8881 ggctatcccg ccggcggcga attccggacc tgggacgggg cggtggcgcc cgcggggacc
     8941 aggaccttca cccacaccgg aaacaaggac gagtgggacg acgccttcgt ccccgatcac
     9001 acagatttct actaccgggt ggatgcggtg acggccgacc cgctggtgcg ggcctcgtcc
     9061 gccgtggtcg gaccggtgca gagcgccggg cagtggttca acacggggaa ggtggcggtg
     9121 ctgatcgcgg tgctgctctt cggcgcgctc atcgtgatcc ttgtggaccg ggcgcggcgc
     9181 ggcgcggacc tctacgtgcg gccgctggcg gggatcgagg cggtcgatga cgccatcggg
     9241 cgggccaccg agatgggacg tccgatcctc tacgtgctcg ggctcgggac ggcggcggac
     9301 gtggcgacca tcgcgtcctt cactgtcctc ggccgggtgg ccaagcgggt ggccgaatac
     9361 cagacctcgc tcaacgtgcc gacctacgac ccgatcgtca tgtcggtggc ccaggaggtg
     9421 gtcaagtcgg cctatctcga tgccggccgg cccgacgact acaaggaaga catcgttcac
     9481 ttcgtcaccc agtcgcagtt cgcctacgtg gcggcggtga acgggatcat gatgcgcgag
     9541 cggcccgcca ccaacgtcta catggggaag ttctacgccg agtcgctgat tctcgcggag
     9601 accggggcgc tggcgggctc gatccagatt tcggggaccg acgagatcgc gcagataccc
     9661 ttcttcatcg tcgcctgcga ctacactctc atcggcgaag agctctacgc cgcctcggcc
     9721 tacctcgggc gcgaaccggt gctgctcggc tcgctcaagg cccaggatta catgaaagcg
     9781 gccgtgattg tcctggcgat catcggcctg ttcgccacca acctcggctg ggccgcgttc
     9841 cgcacgtggt tcaacgtcgg caactgacgg gaggactgcg agtatgaaac gacaaattcc
     9901 cttcctgctg gtcttcctgt tcgcgctctt ccaggtggtc cagtatttcg tgccccacga
     9961 gtctttcgag tggctgtacg agttcttcct cgactggatt tacatcatcg gcattttcgc
    10021 gctggctttg ggaatctggt cgctcttccg cgtgtcggtg gaccggatcc gcagcaagcg
    10081 ccccttctgg gggtacaact acctgacgct catcggcctg ttcctgatga tctcgttcgg
    10141 gttcgtcggg aaagaggcgg gtgcccagtc gtggggaacc tactggctgt ggctcattct
    10201 gtttacggtg ctcctgttgg ccctgatcca gatcggggcg acgggcgacg ccggccggaa
    10261 gaagctctac gccctggtgg ccctcgtcct ggtcgtggtc gagttcctgc tgccggcgct
    10321 ggacaagaac tgggggttct tcttctacac tcaggaagga ctgaccaacc cgatgttccg
    10381 gcacttcttc gactacgtga tgatgccgat tttggcgacg atgttcgcgc tgctcgcgtt
    10441 cttcatcgcc tcggcggcct accgggcgtt ccgggcgcgc aacctgatgg cgacgctgct
    10501 gctgctggcc gcgctcatcg tcatggtgcg tttcaacccc tacctgccgt tcgccgcgga
    10561 gaccgggttc tactccaagc tgtcgaactg gctcatgaat gtgccgaacc aggccgcgca
    10621 gcgggcgatc ctgatcggga tcggcctggg catcgtctcg acgtcgatca aagtcatgct
    10681 cggcatcgaa cgcggctaca tggggagagg gtgaggagga cgccatgagt atatttgaga
    10741 agctgatgaa gctggaccgg cgctgggtct tcctcttcct cgtcgtcgtg tgcgtggtcg
    10801 cctacgcgat gccgttcacg gtgccgattc tggtcgagcc ggaagtgagg agcattttcg
    10861 attttgtcga gcagctgaag cccggcgcga tcattttcat cgcgatcgac tacgatccga
    10921 acagcctggc cgaactgcac cccatggcct acgccatgat cgagcaggcc tggcgccaga
    10981 agaagaggat catcttcacc gcgctctcgc agaacggccc ggggatggcc gaccagttga
    11041 tccgcgacgt gagcgactcg ctcaaagtgg agaaggagta caacggcgtg gtctatccgc
    11101 cccgcgaaat cgtcaacggg atcgactacg ccttcctcgg ctacaagccc tactacggac
    11161 tggtgatcct cggcatgggg caggacttcc gcattccttt cccctccgac tactacggga
    11221 cgccgctgga ctcgctgccc atgatgcagg gggtgatcaa ctacggacag gtcgacctgg
    11281 tcgtggacct gtccagcgga aacatcaccg acgcctggat caactacggg cagggacggt
    11341 tcgggttccc gctcgccctg gggctgacgg gcgtgtcgac cgcccaatac tacccgtacc
    11401 tcggttccgg ccaggtgctc gggatcatgg gcgggctgct cggggcggcc cagtacgagt
    11461 atctggcgga caacccgggc cgggcgatgg acggcatgaa agtacagctc tgggcccaca
    11521 tcgtcatcat cctcttcatc gtcatgggca acgtcgggtt cttcgccagc cgccgtcgcc
    11581 agaagcggga aggaggccag taagatggat ttcgggactt tcctctggtt caccggcggc
    11641 gtgtttctga cgctggcgac cttcagcttc ctgtacaagg acaacccgtt ctacaaggtg
    11701 gctgaacaca tcgtcgtggg cgtctcggcc gggtacttcg tgatcattct ctggcacaac
    11761 gggctggtgc cgaaactgtt ccagccgctg tcggacggca gcgtgtacct cgcttttctc
    11821 gactccaccc aatggtaccg catcatcccg gccctcctcg gcgtgatgat gtggacgcgt
    11881 ttctcgaaga actatcagtg ggtgtcgcgc tggccgatgt cgatgtacat cggtattgcg
    11941 gtcggcgtct ccattccgct cgatctcgga aaccgggtga tccgccagct tcaggcgacc
    12001 atgtacgact tcaagtggga cacctggggc gacttcatcg gcagctccaa cctgttctac
    12061 gtgctggatc cgttcagcgg gctgtcgcag gtgatcatcg cagtcggatc gattgcgggg
    12121 ctgacctact tcttcttttc gaaagcccac accggggtgt tcgggcgggt cgcgaccttc
    12181 ggcatctgga ttctgatgat cgggttcggc gcctcgttcg gattcaccgt gatggcgcgt
    12241 atttcgctgt tcattaaccg cgtgcaggat gtcgacagtt ggcgcaaagt cggctggaac
    12301 gcgggggaaa cacccatcga ggggtactcg gcctggtacc agctggtggg ttggctgctg
    12361 gtggcggtcg tggtggccta tatcgtaaat gagatacggc tgtatttgaa gagccggaat
    12421 caggtcgccg ggccgcaggt ttgagcggac cgaccgggtg acgattaatc ccgccgggtc
    12481 tataaccggc gggatttctt ttttctttga gggttgcaat ttttcgatag ttttttgttg
    12541 atttgagttt atggaattat actgttgggg ctgaagctat tggtctgcat ggaacttttc
    12601 ggggttgttt aacgcatcgc atgaccgaca gacggagttt gaggcaggcg acaatgacca
    12661 ggcagtgtat gttgaaaatt ccggacgctg cagttagcgt actgtcgcac aggggcttcc
    12721 gccgggtcgc gacaatacgc gtaacaggcg ccgggaaagg aggttgtaga agaagagaaa
    12781 acgggatttg tcaagtcatt atgtaaatga agattaccga aaaatatttc gcgtcttcgg
    12841 aaaaaaatgc ttgacataca gtaaggattt cctatcatcc ctgtggagga gccaagcgcg
    12901 accggagtgg agcccggaag cgcatcgtcc gttacgaaaa gggtagccac tacaacacag
    12961 ggaaagtgag gtaggaacgt ggctcggcct cgcataacga ctgtagccaa tcccgtcgcg
    13021 cattcgacta ccgctcccga gaacggggag accagaacaa agctttccac ggccgaggcg
    13081 cgccgagagt atcagcgacg gtactaccag atgcacaagg agaaggcgaa ggagtatcag
    13141 cgccagtaca atctgaagca caagaagaaa gcgcgcggcg gtcgcgggaa agccaattct
    13201 ctctgcccgc gcgaggtggt gcgcacgact ttcaacagct cggacatcat gcatgcgccc
    13261 gccgagaaag cggcgcgcat tctcgacaag atcatcaagg gcgaacgtct cttcacgatg
    13321 tagcgccgcg atggcgctct cgacaggttt caggcggccg gaactcacgt taagtttcgg
    13381 ccgccttttg tgttgcgccg atggagattt ttgtatagat tcgcacaaat tttcacaacg
    13441 cgattcgttg gcgaaaggga cattctatgc cagctaagaa ggcggccaag aagacggtga
    13501 agcagacggc caaacaagcc tccaagaagg atgcgggtcg gaagacggcg cggccgactc
    13561 cgagcgagaa aacgccgaaa aagatcgccc cgccgaaaga tgcgggcggt ctgaaaaaaa
    13621 cggcgcacaa accggcgccg gcggccaaaa gaccggccgg aaagaacgtc gccgtgtcgg
    13681 ccaaaatcgc cgaggccgtg gcgaagggga aattctacta ctacttcggg gacggcaagg
    13741 cggacggcag cgccgacatg aaggctttgc tggggggcaa aggcgcaaac ctggccgaga
    13801 tgagccgggc cggagtgccc gtgccgcccg ggttcacgat caccaccgag gtgtgcaacc
    13861 tctactacga taacaacctg caggtgcccg gcccgatcga cgccgacctc gagaaacacg
    13921 tcggcatgat cgaaaaatcg gtcggcaagg aattcggcaa cccggccgat ccgctcctgg
    13981 tctcggtgcg ctcgggggcg aagttctcca tgccggggat gatggatacc gtcctcaacc
    14041 tcggactcaa cgccgagaca gtcgaaggtc tggcgaaaaa aacgggcagc cagcggttcg
    14101 cgctcgacgt ttaccggcgg ttcatcgcga tgttcggcaa cgtcgtcctg ggcatcgaca
    14161 agagcgagtt cgagcaggtg atcgacaaga agaagaaaga gcgccggatc aagcaggatt
    14221 catcgctgca ggaaggggac ctgcgggaca ttatcaagaa gttcaaacac atctgccggc
    14281 ggaaatccgg cgaggagttc cccgaggatc cgtggcagca actgcgcatg gcgcgcgacg
    14341 ccgtgttccg cagctggaac aacccgcgcg cgatctctta ccggcggatg cacgatatcc
    14401 cgtcggatct cggcacggcc gtgaacattc aggccatggt gttcgggaac atggggaaca
    14461 actctgcgac cggggtgggg ttcacgcgca atcccgccac cgggacgaag gagttctacg
    14521 gcgagtatct cgtgaacgcg cagggagagg acgtggtggc cggcatccgc acgccccagc
    14581 cgatcgtcca gctcaaggag gagatgccgc aggcctacaa gcatctcgtg gaaatcacca
    14641 cccggctgga gaagcactac cgcgacgtgc aggatttcga gttcacgatc gaggagggga
    14701 agctgtatat gctccagacg cgcaacggca agcggacggt gcaggcggcg atcaagatcg
    14761 cggtggacat ggtcaaagag aagctgatca ccaaggagga ggcgctcgag cgggtcgagc
    14821 cgagcagcct cgaccagctc ctccacccgc ggcttgaccc gagagcctcg tacgaggtgc
    14881 tggcgaaggg tctgccggcc tcgccgggcg cggcctcggg gaaggtgtat ttcacggccg
    14941 aggacgtggt caggcacggc gagaagacgc ctgcgatcct cgtgcgcgag gagacgaacc
    15001 cggacgatat cgagggcatg aatgtcgcgg ccggtatcct cacggcgcgc ggcggcatga
    15061 cctcgcacgc ggccgtggtc gcccgcggca tgggaaagtg ctgcgtctcc ggggccgagg
    15121 cgatccgcgt caacccggcc aagaagcagt tccaggtggg caaactcgcg atcaaagagg
    15181 gggagatcat caccctcaac gggaccacgg gcgaggtgat cctcggcgag gtgccgacga
    15241 tcgagccgga gctgtcgggc gaattcagcg agttcatgct ctgggccgat gagatccgcc
    15301 ggctgaaagt gcggaccaac gccgacacgc cgcgcgacgc cgcgcaggcg atcaagttcg
    15361 gcgccgaggg tatcggcctg tgccgcaccg aacacatgtt cttcgccgag gaccgcattc
    15421 ccatcgtgca ggagatgatc ctcgccgaaa cccccgagga acgccaggcg gccctggaca
    15481 aactggtgcg cttccagaag aaggacttca aggaaatctt cgacgccatg caggggaaac
    15541 cggtgaccgt gcggacgctc gatccgccgc tgcacgagtt cctgcccaac cgcgacgagg
    15601 tcaaggcgaa gatcgccaaa ctcgagccga aggacgagga gtacgacgag aagctggccc
    15661 ggctcgagcg cgtgatcgcg cgcatcaacg agctcgccga gaccaacccc atgctcgggt
    15721 tccgcggctg ccggctcggc atcctgttcc ccgaaatcac cgagatgcag gtgcgggcga
    15781 tcatggaggc ggcgtgcgag ttggcgcgcc agaagaaagc ggtgctgccc gagatcatga
    15841 tcccgctggt gggacacgtc aacgagctgc gcaaccagaa ggaggtcgtg ctccgggtgg
    15901 ccaacgaggt ggtcaacaag tacaaggtca aggacctgac ctacctcgtg ggcacgatga
    15961 tcgaggtgcc gcgggcggcc ctgacggccg accagatcgc cgccgaggcc gacttcttca
    16021 gcttcggcac caacgacctc acccagatga cgatggggtt ctcgcgcgac gacgccggac
    16081 ggttcctgcg ccactatgtc gacagcggaa tcctgccgaa ggatccgttc gtgtcgctcg
    16141 accagcaggg cgtcgggcag ctcgtgcgcc tgggggtcga gcgcggccgc cggaccaagc
    16201 ccgatctgaa aatcgggatc tgcggcgagc acggcggcga tccggcctcc atccacttct
    16261 gcaacgaggt cggtatgaac tatgtctcct gctccccctt ccgggtgccg atcgcgcggc
    16321 tggcggccgc ccaggcgacg ctccaggagc agaaggggaa ggggaaaggc cgcaccggga
    16381 ccgagtagga cggcgaggcg ttccggcggc cccacggcgg ccgggacaca aaccagacga
    16441 gaccggccgg cggcgcggca gcgcgtcccg gccggtcaga gtatgaggcg gtatgaaacg
    16501 cgcgctcgat gcgatctttc ggcccaaatc ggtggcggtc atcggggcct ccacgcggcc
    16561 cggcacgatc ggccgcgaaa ccctccacaa catcctcgtc gccgaattca acggcaaggt
    16621 cttcccggtc aacccgaaag cgcccgtgat ccactcgatc aaggcctact cgaccattct
    16681 cgacgtgccg gactcggtcg atctcgccat tgtgatcgtg cccaaggaat tcgtcgccga
    16741 ggtcgcacgc cagtgcggcg aaaaaggggt caaagggctg gtcgtcatct ccgccggttt
    16801 ctccgagacc ggccgggagg ggaagaaacg ggaaatggag ctcgtgcgcg tcgtccagga
    16861 ctacggcatg cgcatggtcg gaccgaactg tttcggcatc gtcaacacca gcccggaggt
    16921 gaatctcaac accactttcg ggaaggtgtt tccgaaacgg ggccgggtcg gcttcatcac
    16981 ccagtcgggg gcgatggggg aggcgatcat gaaccacgcc cgcgagctcg ggatcgggtt
    17041 ctcgctggtc gcctcgatcg gcaacaaggc cgacatctcc tcgaacgaca tcctcgagta
    17101 tttccgcgat gatccggaca cggacatcat cctcatgtac ctggagaatt tcggcaaccc
    17161 gcggcacttc acgctgatcg cccgggagtt gtcgcggacg aagccgatcg tggcggtgaa
    17221 atcggggcgg acgacgctcg gcgccaaggc ggcctcgtcg cacaccggcg ccctggccgg
    17281 gctcgacgtg ggtgtggacg cgctcttcga ccagaccggc gtgatgcgcg tcgacacggt
    17341 ggaggagctg ttcgacgtgg cgaaggcgct gtcgatgcag ccggtgccgc aggggaaccg
    17401 ggtggcggtg gtgaccaacg ccggcggccc ggggattctc gccaccgacg ccctgatcaa
    17461 caacgggatg gagatgccgc cgctgtcgcc cgagacggtg aagcggctgc ggcagttcag
    17521 ttccgaggac gcgtcgttcg ccaacccgat ggacatggtg gccggggccg gtcccgagca
    17581 gttccgcagg acgctcggcg ccatcatcac cgacaagcgc tacgacagca tcatccccat
    17641 cttcgtcccg ccggtgacga tcgacgaact cgaggtggcg cggagcatcc aggaggcggt
    17701 ggccgggacg aagaagacgg tgctcgcctg tttcatgggg gccagccgca attcggccgg
    17761 catcgaacac ctccgcagcc acggcatccc cgtctacatc taccccgagg cgatcgcgaa
    17821 gacgctggcg acggtgagcc actaccgccg gcgcatgaat gccccccggg ggaaagtccg
    17881 gaccttcgag gttgacccgg accgggtgca ggcggtcatc gagcgggcgc gc
//
LOCUS       DAOPOE010000066        17910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3762_length_17910_cov_5.067880, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000066 DAOPOE010000000
VERSION     DAOPOE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17910
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3762_length_17910_cov_5.067880"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKM94_09475"
     CDS             <1..299
                     /locus_tag="PKM94_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66908.1"
                     /translation="GVEAIPDPDAAVCAVEQLAAPAPAETAGPGLEVSESAFFRFEQR
                     GDYPAMLRAAVLLQRAGHPKAAAFIERAACAALDRGDVSFAWNALLYLGRLGRA"
     gene            453..2411
                     /locus_tag="PKM94_09480"
     CDS             453..2411
                     /locus_tag="PKM94_09480"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOD66909.1"
                     /translation="MTAPAPSPLWVPPPSRVERSHLSRFMCFLGRRARREFFSYDDLW
                     QFSVDHIASFWEAVWDFAGIIHSQPWEAVVEGDRIDNAVWFRGTRLNFAENLLRYRDR
                     RTAIISECELGTVETITYADLYGRVARCAAGLKKMGVGPGDRVAAFIPNIPEAVVAML
                     AATSLGAVWSSCSPDFGLQGVLDRFGQIEPKVLITADGYQYNGRRYDSLATVARVLEH
                     LPGAARAVVIPRLGPFDPAALPNSLAWAELLANDAAEIEFRQLPFDHPVYIVYSSGTT
                     GPPKGLVHGAGGTLLQHYKEHVLHTDLGRDDVITYYTTCGWMMWNWLASALQVGATLY
                     LYQGSPSYPDLTRLWCAAERRGITVFGTSPKFLAACEQAGLAPGKMFDLAPLRTILST
                     GAPLSPRSFAWVYENVKRDLQLASISGGTDILSCFMLGCPLLPVYPGEIQCRGLGMKV
                     ETCDDRGRPVAGAVGELVCTERFPSRPVSFWNDPDGSKYRRAYFAHFPGVWRHGDFVE
                     ITPRGGVIVHGRSDATLNPGGVRIGTAEIYAPVEALPEVVDSLAVGQRWRGDTRIILF
                     VRLAPGVALTDDLRGRIGAAIREARTPRHVPAKIIAVDDIPRTRNGKKVEVVVTRLIH
                     GLDVPGRAALANPEALDLFRRLPELDRP"
     gene            2521..4311
                     /locus_tag="PKM94_09485"
     CDS             2521..4311
                     /locus_tag="PKM94_09485"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HOD66910.1"
                     /translation="MAPEPAPAAPAYRDNPILAALPGHLAQFIVDQNYSAYTPVDHAV
                     WRYVMRQNYAFLKDHAHPAYVDGLRRTGIGIEKIPSIEDMNEILGRLGWAAVPVDGFI
                     PPAAFMEFQAHNVLVIAAAMRQIDHIEYTPAPDIIHEAAGHAPIIADPEYADYLRRIG
                     AIGAKAMSSKKDYELYEAIRRLSILKESDRPDPAAVARAEQDVAAKQAGLGPPSEMAR
                     LSRLHWWTVEYGLIGDLDCPRIYGAGLLSSIGESASCLGPEVRKIPYTLDAADYPFDI
                     TTQQPHLFVTPDFARLTEVLEQFAATMAFRVGGAEGLAKALECENVSTAQFSSGLQVS
                     GVVAEVRTRRPGAPPCFFRTAGPTALALDGRQLAGHGRERHRDGFSSPIGTLRGQAAP
                     LERMSDSDLAACGLEPGRPAAIEFESGLRLSGRLESTLRSPDRALLLMTFSDCTVADG
                     SAVLFRPEWGPYDMAVGASIVSVFAGAADKSAYQQPSLVPRERTVTADRPDAALRLLD
                     LYRQVRDVREGQRGLAALPAVWEALQAEYPCEWLLPLEILEICRQRGLHPELAAALRR
                     YLERKALAEPGLAKLIRDGLALLDSAAAPN"
     gene            4354..4692
                     /locus_tag="PKM94_09490"
     CDS             4354..4692
                     /locus_tag="PKM94_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOD66911.1"
                     /translation="MSENLAFRHCVPCEGGVDPMTRKDFEVYLEQVAAWRISDNDTKL
                     ERDFTFKDFARAVKFINALAAVAEEEGHHPDIFLHGWNKVRVTLWTHAIGGLSINDFI
                     LATRTDRIEV"
     gene            complement(4817..5527)
                     /locus_tag="PKM94_09495"
     CDS             complement(4817..5527)
                     /locus_tag="PKM94_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD66912.1"
                     /translation="MTAGGAPGRVLITGASRGIGRALAVRLAGPGRTIVVQGRDRRAL
                     EETVRLVRGKGGIGEAIAARLDTAQGAADVLAALGKGPVEVLVNNAGVSRVAPVGELT
                     LAQWEESLAVNVTAPFLLMKGLLPAMGRGSSIVNVLSAANKATFPGWGAYTMSKAALE
                     GLARVVREEVRGRGIRVIDVYLAATATDLWERVPGEWNRAAMLRPEEAAEAIAYALER
                     PGEVLVESITVGGIGGNL"
     gene            complement(5524..6762)
                     /locus_tag="PKM94_09500"
     CDS             complement(5524..6762)
                     /locus_tag="PKM94_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOD66913.1"
                     /translation="MYLTISKKFEISLSYRWWQKDWPAQRNRAFYGPAAGGPHGYGAN
                     ASVHFVFHGPVDPENGMVINVAEVKERVGGMLAARYDHRYLNVDTRPFDEVVPTPENI
                     ARQLWADAQPLFGDAGAGLAAVHLEMSPLDSATAYADGRVERGYGLEFSAARRTWSPR
                     LTEEENTRLFGAAASPSGHGHCYYLRATAAGEPDRESGMIVAPETSGKALAAVFQLLD
                     HRNLVTDVPELAGRPLTTESLSGFLFARLANRMPLARMRLWENPYFYTDCLPTGVYVM
                     GVRADFRAAHRLASPRYSREENERTYGKCTNAAGHGHRYVVEAAIDGYLDEASGALYP
                     LEDLTRGVRTAVAPWDYVHLDEDTDEFAERPSTGENIVSALWPKVEDALGRPLYRLRL
                     WETPNNRFTLRRETPGGAGR"
     gene            complement(6828..7376)
                     /gene="folE"
                     /locus_tag="PKM94_09505"
     CDS             complement(6828..7376)
                     /gene="folE"
                     /locus_tag="PKM94_09505"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOD66914.1"
                     /translation="MLMEDLYRQLMIQIGEDPNREGLRNTPRRAARAFEFLTRGYKED
                     IDAIVNNALFEADTKEMILVRNIEMYSMCEHHLLPFIGKCHVGYIPNRKVLGLSKVAR
                     IVDVYARRLQIQEKLTKEIGEAIQKYTNALGVAVVIEAQHLCMMMRGVEKQNSVMTTS
                     YIIGEFHEDNATRAEFFSLINK"
     gene            complement(7607..8656)
                     /locus_tag="PKM94_09510"
     CDS             complement(7607..8656)
                     /locus_tag="PKM94_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOD66915.1"
                     /translation="MATLPFAQALQERLLVLDGAVGTMLLNHGLREEDFRGEEFAREE
                     PAREGEIGAGDPGAAGTSLRLYGNLDLLTLVRPDLVEAVHRAYINAGAEIITTNTFTA
                     NGPMQARYGTAHLAERMNREAAALARRAADDARDAQPEREVYVAGSLGPIAPGPPAAG
                     GNEGARGSEVSGEDMRRAYAEQVRGLAAGGAEVILIETAMSVAGAAAACAGAQDAFAR
                     LGRALPVWVSATTDETGARMGSGESFTEFVRAVADFGPVCVGLNCGYGPKSIAEGVRA
                     LAEVTDLPIAAYPNAGLPDEQMVFPVGPPAFGRWAEDLAATGLVRVIGGCCGTTPEHI
                     RAVRRGIGTRGDGRQ"
     gene            complement(8894..10474)
                     /locus_tag="PKM94_09515"
     CDS             complement(8894..10474)
                     /locus_tag="PKM94_09515"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOD66916.1"
                     /translation="MTLTRVNASAATLTKNRTEDSVVPTSGMCVTCVDGCIGMCEIGK
                     SAYRGHEVIYPQPFGVITTAGEKTYPVDYSHFNIMGTAVGAHGIAADSDKAVFPNVNL
                     EVRFGHDRGIKFRLPWIIPGIGSTDIAKNNWEGLAIGSALAGTGLTIGENVVGMDVEA
                     AIKNGRVVDTVDLKRRVKLYQDHQREGYGAIIVQANIEDTRLGTQEYAIEKLGVSIVE
                     LKWGQGAKNIGGEVKIRDLKKAQLLYERGYVVLPNPTDPKVIEAFQHGAFKEFERHSR
                     VGMVTEEGFAGRVEELRKAGAKYVFLKTGAYRPADLARAIKWSSKYGIDLLTVDGAGG
                     GTGMSPWRMMNEWGIPPVELHTLTHQYCTRLVKKGERVPAIAVAGGFTFEDQIFKGLA
                     LASPYVKLVGMARAPIAAAMVGKTIGRTIETNQVPVYVERFGTTKDEIFVTAHELRHL
                     LGNEAFEKLPTGALGLYTYYERLAQGLRQLMAGGRKFATEYITRDDICALTHEAAELS
                     GVQYVMDVDKKEVDAIMN"
     gene            complement(10495..11178)
                     /locus_tag="PKM94_09520"
     CDS             complement(10495..11178)
                     /locus_tag="PKM94_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD66917.1"
                     /translation="MTTLAINGLRVQVETGSTILEAAQFLGFPIPTLYHMEGLTPYGA
                     CRLCVVEIGEGPAARLVTSCTYPVQEGLTVRTASERVLRARRMIIELLLASCPQSKVI
                     QDIASAHQVRQQRFRQEHEDCILCGLCVRMCKEQMVAQAIGFYGRGEHRGIGTPFDVK
                     SEVCRLCGGCMYVCPACQLRCTYTDPEKAICGGCANLTPPCVEKEQFHDMMCYMKPCV
                     ACEIPKDAK"
     gene            complement(11175..14387)
                     /locus_tag="PKM94_09525"
     CDS             complement(11175..14387)
                     /locus_tag="PKM94_09525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD66918.1"
                     /translation="MARKLTSVDDLAGFRSRVMAERRRAEAIPTIVVSADTGAQASGV
                     NDLIRLFKKHMLDRFLQEKLRLRITGCHGFCEMNPYVTVEPYGHFYPRLRPADVPRII
                     EAALSGQPADDLLYRDAATGTVYPRQSDIPFFARQKRTIVGNNQKLDPIRIMDYFENG
                     GYGALLKALEKNDPEWIIQEVQTAGIRGRGGAGFPTGRKWALARTSGRPGQQKYIVCN
                     ADEGDPGAYMDRGVLEGNPHAIMEGMVIGGLAIGATRGIIYVRSEYPLAIKHCLIAIR
                     QARDLGLLGEKILGTEHDFDIEIVRGAGAFVCGEETALIKSIEGYMGEPRQRPPFPIE
                     RGIFGSPTCINNVETFANIPEIITRGGAEYAKVGLPGNTGTKIFSLVGKIRNTGLVEV
                     PLGTAISEVVYGIGGGAAGKAKIKAVQTGGPSGGCIPASMFDLSIDYDSLTKAGSIMG
                     SGGMIVMDENTCMVDLAKYFMKFLKDESCGKCFTCRKGTQRMWEILDDITKGKATMEH
                     LDLLAELALVVKDTSMCGLGQSAPNPVLSTIRYFRQEFERHILDKRCDAFVCTELVGA
                     PCQSACPIGTEAWRYVAHIANGEYKEAYAVIREANPFPSVCGRVCHHPCEERCKAGQT
                     GTNPVAIRALKRFITDRIDPSAYTPRREVWTDGPAPKVAVIGSGPAGLTAAHYLSLKG
                     YHVTVFEKEDRPGGMLYCAIPGYRLPQEVIRREIEAVLNDNITVRCRMALGREITVDG
                     LLGEGYRAVLLALGAHESKPLKLENEDCAGVHPSIEFLKAFNQRGEARARGRVGVIGG
                     GNSAFDAARVALRQRDVESVTILYRRTREEMPAFAEEIEAAAQEGIDIQILVTPKKVL
                     AKDGKLIGLECLRNRLGEIDESGRHRPVPIAGSEFRVDLETLIVAISEDSGVDAIGPA
                     KQSGIAITDWNTVKVDKKTLLTTRPGVFAAGDVVRGPNTVVDAIADGKRAARMIDRYV
                     RGLELADAAEPALPRVYVPPVSAELDDLPTVERVETPRAPAAWRTRNFAEVEVALSVA
                     EAHCEARRCLRCDLEFTRPPETGQETDHDGAVAVTGGKQL"
     gene            complement(14422..15273)
                     /locus_tag="PKM94_09530"
     CDS             complement(14422..15273)
                     /locus_tag="PKM94_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD66919.1"
                     /translation="MNTQEVLSIVEKHHGNRGSLISILEDMQAKYGYLPEAMLRVVAE
                     KTGRSLVDIYGVATFYRAFSLRPRGKHLVSCCLGTACHVRGGPAIAREIEKRLGIRAG
                     ETTADREFTLETVNCLGACALGPIVVVDGHYFSKVKTSMIPDILARAKEGLDAVRVET
                     DERIFPVEVSCARCNHSLMDPRHEIEGHPSVRVTASFGSKHSWLAISSLWGSYTVHSE
                     HEIPAETVVTFFCPHCHAEMMGGAPCAECGAPMVPMIVRGGGIVQLCSRRGCKGHILD
                     FGGTAAD"
     gene            16245..17747
                     /locus_tag="PKM94_09535"
     CDS             16245..17747
                     /locus_tag="PKM94_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD66920.1"
                     /translation="MSDNDSSLAGRAAVAMALTIGFYSLALILGLGTILLPFIIAWAT
                     GQLILKLVIVCLISGGAILAAIVPRRDQFEPPGPELSLAEHPRLAEVIRRVAGQTEQA
                     MPRTVYLIPDFNAWVAQRGGSHLHGGDRLMGIGLPLLHQLTVSQFEAVIAHEFGHYHH
                     GDTRLGPRIYQTRAAIVRTIAGLHEHNSILQAPFLWYGRMFLQITHAISRRQEFNADR
                     LAAQVAGTAAMRSALEKINRGGIAFDAYWQSEFVPMLRSRRLPPLLDGFTLFLQCEPM
                     QTRIDACAEQVLASEATDKYDTHPSLRERLAALEALPAGPALSDRRPALDLLSDPAEQ
                     ERELLRAIVAAGEFERLTPVGWDEVAPIHAARWQAMSRERRHFFFAITPKDLPRFVRD
                     PKPFVKDRPLQDLLAGPNQAQAAEAVAVTVALALASLLVRLGWTLERPIGSNPRMVLG
                     AAVIEPFDTLDLLRSGQLPAPEWLRLCRDTGIGDHPLFPPSHKEDGARAE"
BASE COUNT         2670 a         7305 c         4919 g         3016 t
ORIGIN      
        1 ccggggtcga ggcgattccc gaccccgacg ccgccgtgtg cgcggtcgaa cagctcgccg
       61 cccccgctcc cgcggagacc gcgggccccg gcctcgaggt ctccgagtcc gcgttcttcc
      121 gtttcgagca gcgcggcgac taccccgcga tgctccgcgc cgccgtgctg ctccagcgcg
      181 ccggccaccc gaaagccgcg gctttcatcg aacgcgccgc gtgcgccgct ctcgaccgcg
      241 gcgacgtctc cttcgcctgg aacgcgctcc tctatctcgg ccgcctgggc cgcgcctagc
      301 cgccgccggc tccggccaaa aaatcgattg atataatcca ttttccgtga acccgtcgcc
      361 cccgccgggt gttctgtcag gcggacgccg ggcgcgcggc cgcgcccgcc cggccggcac
      421 tgaggagaac cgctgttgga gaacccgctc ccatgaccgc ccctgcccca tcccccctct
      481 gggtcccccc gccgtcccgc gtcgagcgca gccacctgtc ccgcttcatg tgcttcctcg
      541 gccgccgcgc gcgccgcgag tttttctcct acgacgacct ctggcagttc tccgtcgacc
      601 acatcgcgtc cttctgggag gccgtctggg acttcgccgg catcatccac tctcagccct
      661 gggaagcggt cgtcgagggc gaccgcatcg acaacgccgt ctggttccgc ggcacccgcc
      721 tgaatttcgc cgagaacctc ctccgctacc gcgaccggcg caccgccatc atctccgagt
      781 gcgagctggg gacggtcgag acgatcacct acgccgacct ttacgggcgc gtcgcgcgct
      841 gcgccgccgg cctgaaaaag atgggcgtcg gtccgggcga ccgcgtcgcc gcctttatcc
      901 ccaacatccc ggaagctgtc gtggccatgc tcgccgcaac cagcctcggc gccgtctggt
      961 cctcgtgttc gcccgatttc ggcctccagg gcgtgctcga ccgcttcggc cagatcgaac
     1021 cgaaagttct catcaccgcc gacggctacc agtacaacgg tcggcggtac gactcgctcg
     1081 cgaccgtcgc ccgcgtcctc gaacacctcc ccggcgccgc ccgggccgtc gtcatcccgc
     1141 gcctcggccc gtttgacccc gccgcgctgc cgaacagcct cgcctgggcc gaactgctgg
     1201 ccaacgacgc cgccgagatc gaattccgcc agctcccctt cgaccacccc gtctacatcg
     1261 tctactcctc cggcaccact ggccccccca agggcctcgt ccacggcgcc ggcggcaccc
     1321 tgctgcagca ctacaaggaa cacgtcctcc acaccgacct tggccgcgac gacgtcatca
     1381 cgtactacac cacctgcggc tggatgatgt ggaactggct ggccagcgcc ctccaggtgg
     1441 gcgcgacgct atacctctac cagggctccc cctcctatcc cgacctcact cgcctctggt
     1501 gtgcggccga gcgccggggc atcaccgtgt tcggcacgag cccgaaattc ctcgccgcct
     1561 gcgaacaggc cggcctcgcc cccggcaaga tgttcgacct cgctcccctc cgcaccatcc
     1621 tctccaccgg cgcgccgctc tccccgcgga gcttcgcctg ggtgtacgag aacgtcaagc
     1681 gcgacctcca gctcgccagc atctccggcg gcaccgacat cctctcctgc ttcatgctcg
     1741 gctgccccct cctccccgtt taccccggcg aaatccagtg ccgcggcctc ggcatgaaag
     1801 tggaaacctg cgatgaccgc ggccgcccgg tcgccggcgc cgtcggcgag ctcgtctgca
     1861 ccgaacgctt cccctcccgt cccgtctcct tctggaacga ccccgacgga agcaagtacc
     1921 gcagggccta cttcgcacac ttccccggcg tctggcgcca cggcgacttt gtcgagatca
     1981 ccccgcgcgg cggcgtcatc gtccacggcc gctccgacgc cacccttaat cccggcggcg
     2041 tccgcatcgg caccgccgaa atctacgccc ccgtcgaggc cctccccgaa gtcgtcgact
     2101 ccctggccgt cggccagcgc tggcgcggcg acacccgcat catcctcttc gtccgcctcg
     2161 ccccaggcgt cgccctcacc gacgacctgc gcggccgaat cggcgcggcc atccgcgagg
     2221 cccgcacccc gcgccacgtc ccggccaaaa tcatcgccgt cgacgacatc ccccgcaccc
     2281 gtaacggcaa gaaagtcgag gtcgttgtca cccgcctcat ccacggcctc gatgtccccg
     2341 gccgcgcggc cctcgccaac cccgaggccc tcgacctctt ccgccgcctc ccggagctcg
     2401 accggccctg acccccctca cttttccctt gacgcgcgcc gcgcgcgccc cctattataa
     2461 tcgcaataat agattcatat aatccatgta tcgccgctcc gcgcggaaag gacgtacgcc
     2521 atggctcccg aacccgcccc cgccgccccc gcctaccggg acaaccccat cctcgccgcc
     2581 ctgcccggcc acctcgccca gttcatcgtc gatcagaact actccgccta caccccggtc
     2641 gaccacgccg tctggcgcta cgtcatgcgc cagaactacg cgttcctcaa ggaccacgcc
     2701 caccccgcgt atgtcgacgg cctgcgccgg accggcatcg gcatcgagaa gatccccagt
     2761 atcgaggaca tgaacgagat cctcgggcgc ctcggctggg ccgctgtccc cgtcgacggt
     2821 ttcatcccgc cggccgcctt catggaattc caggcgcaca acgtcctcgt catcgccgcc
     2881 gccatgcggc agatcgacca catcgaatac acccccgcgc ccgacatcat ccacgaggcc
     2941 gccggccacg ccccgatcat cgccgacccc gaatacgccg actacctccg ccgcatcggc
     3001 gccatcggcg cgaaagccat gtcgtcgaaa aaggactacg aactctacga ggccatccgc
     3061 cgcctgtcga ttctcaagga gtccgaccgc cccgaccccg cggccgtcgc ccgcgccgaa
     3121 caggacgtcg ccgccaaaca ggccggcctc ggcccgccct ccgaaatggc ccgcctctcc
     3181 cgcctccact ggtggaccgt cgagtacggc ctcatcggcg acctcgactg tccgcgcatc
     3241 tatggcgccg gcctgctctc ctccatcggc gagtccgcct cctgcctggg tcccgaggtg
     3301 aggaagatcc cctacacgct cgacgccgcc gactacccct tcgacatcac gacccaacaa
     3361 ccccacctct tcgtcacccc cgatttcgcc cgcctcaccg aggtcctcga gcagttcgcc
     3421 gcgaccatgg ccttccgcgt cggcggggcc gaggggctgg ccaaagccct tgagtgcgaa
     3481 aacgtctcta ccgcccagtt cagctccggc ctccaggtgt ccggcgtcgt ggccgaggtc
     3541 cgcacccggc ggcccggcgc accgccctgc tttttccgca ccgccggccc gaccgcgctc
     3601 gccctcgacg gccgacaact tgccggccac ggccgcgagc gccatcgcga cggcttctca
     3661 tcgcccatcg gcacactccg cggccaggcg gcgccgctcg agcggatgag cgacagcgac
     3721 ctcgctgctt gcggcctcga acccggccgc cccgccgcca tcgagttcga aagcggcctc
     3781 cgcctcagcg gccgcctcga atccactctc cgcagccccg accgcgccct cctcctcatg
     3841 actttctccg actgcaccgt ggccgacggc tccgccgtcc tcttccgccc cgagtggggc
     3901 ccctacgaca tggccgtcgg cgcctccatc gtctccgtct tcgccggcgc cgccgacaaa
     3961 tccgcctacc agcagccctc cctcgtcccg cgcgagcgga ccgtcacggc cgaccggccc
     4021 gacgccgccc tccgcctcct cgacctctac cggcaggtcc gcgacgtgcg cgagggacag
     4081 cgcggcctgg ccgcactccc ggccgtctgg gaggccctcc aggccgagta cccgtgcgag
     4141 tggctccttc ccctggagat tcttgaaatc tgccggcagc gcggcctcca cccggaactc
     4201 gccgccgcgc tgcgccgtta tctcgaaagg aaagccctcg ccgaacccgg gctggccaaa
     4261 ctgatccgcg acggattggc gctgctggac agcgccgccg cgccaaattg acggccgaac
     4321 acagccggct caaactgcgg agttgtgaac cctatgtccg agaacctcgc cttccggcac
     4381 tgcgtcccct gcgagggggg cgtcgatccc atgacgcgca aggattttga agtctacctg
     4441 gagcaggtgg ccgcttggcg catcagcgac aacgacacaa aactcgagcg ggatttcacg
     4501 ttcaaagatt tcgcgcgggc ggtgaaattc atcaacgccc tcgccgccgt cgccgaagag
     4561 gagggccacc atccggacat cttcctgcac ggttggaaca aggtccgcgt caccctgtgg
     4621 acccacgcca tcggcggtct ctcgatcaac gacttcatcc tcgccacccg gaccgaccgc
     4681 atcgaggtct gacaccctcg cactgcgggt ccggatccgc gggcctgaag ttcgcggccg
     4741 ggcgcacgtc tgaggcgccc ctttctgaat ttgcaccgcg gggattccag actccgaccg
     4801 gatcgccggg gccttcttac aggttgcccc cgatcccgcc caccgtgatg ctctccacga
     4861 gcacctcccc cggccgctcc agcgcgtagg cgatcgcctc cgccgcctct tccggccgca
     4921 gcatggccgc ccgattccac tcgcccggca ccctctccca caggtcggtc gcagtggccg
     4981 cgaggtagac gtcgatcacc cggatccccc ggccgcgcac ttcctcccgc accacccgcg
     5041 ccagcccctc gagcgccgcc ttgctcatcg tgtaggcccc ccacccggga aaggttgcct
     5101 tgttcgccgc cgacagcacg ttgacgatcg agctgccccg ccccatggcc ggcagcagcc
     5161 ccttcatgag caggaacggc gccgtcacgt tgaccgccag cgactcctcc cactgggcga
     5221 gcgtgagctc ccccacgggc gcgacccggc tcacccccgc gttgttgacc agcacctcga
     5281 ccggtccttt tcccagcgcc gcgagaacgt cggccgcacc ctgggccgtg tcgagccggg
     5341 ccgcaatcgc ctccccgatc ccccctttcc cccgcaccag gcgcactgtc tcctccagcg
     5401 ccctccggtc gcggccctgg accacgatcg tgcgtccggg ccccgccagc cgcaccgcca
     5461 gcgcccgccc gatcccgcgg ctggcccccg tgatcagcac ccgccccggc gccccgcccg
     5521 ccgtcatcgc cccgcccctc ccggcgtttc ccgccgcagc gtgaaccggt tgttcggcgt
     5581 ttcccacagc cgcagccggt acagcggccg ccccagggcg tcctccactt tgggccacag
     5641 cgcgctcacg atattctccc cggtgctcgg ccgctcggcg aactcgtccg tgtcctcatc
     5701 caggtgcaca tagtcccagg gcgccaccgc cgtgcgcact cccctcgtga gatcctccag
     5761 cgggtagagc gccccgctcg cctcatccag gtatccgtcg atcgccgcct cgaccacata
     5821 gcggtgcccg tgcccggcgg cgttggtgca ctttccgtac gtgcgctcgt tctcctcccg
     5881 cgagtaccgc gggctggcca gccggtgcgc ggcgcggaaa tcggcccgca cccccatgac
     5941 atacacgccg gtgggcaggc agtcggtgta gaaatatggg ttctcccaca gccgcatgcg
     6001 tgcgagcggc atccggttgg ccagccgcgc gaacaagaac cccgacagcg attccgtcgt
     6061 cagcggccga cccgccaact ccggcacatc ggtcacgaga ttccggtggt cgagcagctg
     6121 aaacaccgcc gccagcgcct tgccgctcgt ctccggcgcc acgatcatcc ccgactcccg
     6181 gtccggctcc cccgccgccg tcgcccgcaa atagtagcag tgcccgtgcc ccgaggggga
     6241 ggccgccgcc ccgaacagcc gcgtgttctc ctcctcggtc agccgcggcg accaggttcg
     6301 ccgcgccgcc gagaactcca gcccgtaccc gcgctccacc cgcccgtccg cgtaggccgt
     6361 cgcgctgtcc aggggcgaca tctccaggtg caccgccgcc agccccgccc ccgcatcgcc
     6421 gaacagcggc tgggcgtcgg cccacaactg gcgcgcgata ttctccggcg tcggcaccac
     6481 ctcgtcgaac ggccgcgtgt cgacattcag gtaccggtga tcgtaccgcg cggcgagcat
     6541 cccccccacc cgctccttca cctccgcgac attgatcacc atcccgttct ccggatcgac
     6601 cggcccgtgg aacacgaaat ggaccgatgc gttggcgccg taaccgtgcg ggccgcccgc
     6661 cgccggaccg taaaacgccc ggttccgctg cgccggccag tccttctgcc accagcggta
     6721 cgacagggag atctcgaatt tcttgctgat cgtcagatac atgcgcgtcc tttccgccgc
     6781 cgtcgttttt cccccgcccc tccggtcgcc cccggcccgc ccggccctta cttgttgatc
     6841 aggctgaaaa actccgcccg ggtcgcatta tcctcatgga actcccctat gatatacgac
     6901 gttgtcatca ccgagttttg cttctccacc ccccgcatca tcatgcacag gtgctgcgcc
     6961 tcgatcacca ccgccacccc cagcgcgttt gtgtacttct ggatcgcttc cccgatctcc
     7021 ttggtcagtt tctcctggat ctgcagccgc cgcgcgtaga catccacaat ccgcgccacc
     7081 ttcgacagcc ccaacacctt ccggttcggg atatacccga cgtggcattt cccgataaac
     7141 gggagcaggt ggtgttcgca catcgagtac atctcgatat tccgcacgag aatcatctct
     7201 ttcgtgtccg cctcgaacag ggcgttgttc acgatcgcgt cgatgtcctc tttgtagccc
     7261 cgcgtgagaa actcgaatgc ccgcgccgcg cgccgcggtg tgttgcgaag cccctcccgg
     7321 ttcggatctt cgccgatctg gatcatcaac tgtcggtaca gatcttccat cagcatacgg
     7381 gtcacctgtc cttccctgag ttgtttgatc ggcgttctct ccggccctgc cgccgccgcg
     7441 ggccgcccct cccggtaggc tccgtccgcc cccgaccgcc gggaccggtt cgccggtccg
     7501 caccgccgta gtatacctgc cctccgcccc cgacacaaac gcaaaagcgc cccgcggcgc
     7561 cgccctgggg gcgcattcgc ggggccgcga tcgcgctccg gcgagcctac tgccggccgt
     7621 cgcccctcgt gccgatcccc cgcctcaccg cccgaatgtg ctccggcgtc gtcccgcaac
     7681 atcctccgat cacccgcacc agcccggtcg ccgccagatc ctccgcccac cgcccgaacg
     7741 ccgggggccc caccggaaac accatctgct cgtccggcaa ccccgcgttc gggtacgccg
     7801 cgatcggcag atccgtgacc tctgccagcg cccgcactcc ttctgcaatc gacttcggtc
     7861 cgtacccgca gttgagcccg acacacaccg gcccgaaatc cgccaccgcg cgaacgaact
     7921 ccgtgaaact ttcccccgac cccatccgcg cgcccgtttc gtcggtcgtc gccgacaccc
     7981 acaccggcag cgctcgcccc aaccgggcaa acgcgtcctg cgcccctgcg cacgccgccg
     8041 ccgcacctgc cacgctcatg gccgtctcga tcagaatcac ctccgccccg ccggccgcca
     8101 gcccgcgcac ctgctcggcg taagcccgcc gcatatcctc cccggacact tcgctcccgc
     8161 gcgccccctc gttgccgccc gccgccggcg gcccgggcgc gatcggtccg agcgaacctg
     8221 cgacatacac ttcccgctcg ggctgcgcgt cgcgggcgtc gtcggccgcc cgccgcgcca
     8281 gcgccgccgc ctcccggttc atccgctccg ccaggtgcgc cgtcccgtac cgcgcctgca
     8341 tcggcccgtt cgccgtgaac gtgttggtcg tgataatctc cgcccccgcg ttgatgtagg
     8401 cccggtgcac cgcctccacc aggtccggcc gcacgagtgt gagcaggtcg agattgccgt
     8461 acagccggag cgaagtcccc gccgcccccg ggtcgcccgc gccaatttcc ccctcccgcg
     8521 ccggctcctc gcgggcgaat tcctcccccc ggaaatcttc ttcccgcaga ccgtggttca
     8581 ggagcatcgt cccgaccgcg ccgtcgagca cgagcagccg ctcctgcagc gcctgcgcaa
     8641 acggtagtgt cgccatatcc ctgctcaact cctgccgatt ctgcttcgac cggcccgctc
     8701 cgccgggctt gcccggaccg caagccgact ctcccaacta gcccgccctt cgcgatgctg
     8761 tcaatcttaa atccccccgc cgggccggag gaagccggcg gacgatcggc ccgtccgccc
     8821 cttcatacgc gcgcggcggc cgactgccgc gaccgccgcg cactttacag catgagcaag
     8881 aggggattct tcgctaattc atgatcgcgt ccacttcctt cttgtccacg tccatcacgt
     8941 actggacccc ggagagctcg gccgcctcat gggtgagcgc gcagatgtcg tcgcgcgtga
     9001 tatactcggt cgcgaacttc cgcccgcccg ccatgagctg ccgcagcccc tgggcgagcc
     9061 gctcgtagta cgtgtacaga ccgagcgccc cggtcggcag cttctcgaac gcctcgttcc
     9121 cgaggaggtg ccggagttcg tgggccgtga cgaaaatctc atccttggtc gtgccgaacc
     9181 gctcgacata caccggcacc tggttcgtct cgatcgtccg cccgatcgtc ttgcccacca
     9241 tggccgcagc gatcggcgcc cgggccatcc cgaccagctt cacatagggt gaggccagcg
     9301 ccagcccctt gaaaatctgg tcctcaaacg tgaacccgcc cgccacggcg atcgccggca
     9361 cccgctcccc cttcttcacc agccgcgtgc agtattggtg ggtcagcgtg tgcagctcga
     9421 ccggcgggat cccccactcg ttcatcatcc gccacgggct catccccgtg ccgccgcccg
     9481 cgccgtcgac cgtgagaagg tcaataccgt acttggagga ccacttgatc gcccgggcga
     9541 ggtcggccgg acggtaagcg ccggtcttga ggaacacgta cttggccccc gccttgcgca
     9601 gctcctcgac ccggccggcg aacccctcct ccgtcaccat cccaacccgc gagtgccgct
     9661 cgaactcctt gaacgccccg tgctggaagg cctcgatcac cttcggatcg gtcgggttcg
     9721 gcagcaccac atacccgcgc tcgtagagca gctgggcctt cttgaggtcg cggatcttca
     9781 cctcgccgcc gatattcttc gccccctgcc cccacttgag ctcgacaatc gaaaccccca
     9841 gcttctcgat cgcgtactcc tgcgtcccca gccgggtgtc ttcgatattg gcctgcacga
     9901 tgatcgcgcc gtacccctcg cgctggtggt cctggtacag cttcacgcgc cgcttcagat
     9961 cgaccgtgtc gacgacccgg ccgttcttga tcgccgcctc cacgtccatg cccacgacgt
    10021 tctcgccgat cgtcagcccc gtcccggcca gcgccgagcc gatcgccagc ccctcccagt
    10081 tgttcttggc gatgtcggtc gagccgatcc ccgggataat ccaggggagc cggaacttga
    10141 tcccgcggtc gtgcccgaac cgcacctcga gattcacatt ggggaacacc gccttgtcgc
    10201 tgtcggccgc gatcccgtgc gcccccaccg ccgtgcccat gatgttgaag tgcgagtagt
    10261 cgaccgggta ggtcttctcc cccgccgtcg tgatcacccc gaacggctgc gggtagatca
    10321 cctcgtgccc ccggtaggcc gacttcccga tctcgcacat cccgatacac ccgtccacgc
    10381 aggtgacgca catgcccgag gtcgggacca ccgaatcctc cgtccggttc ttcgtcagcg
    10441 tggccgccga ggcgtttact ctggtcagcg tcatgatgtc ctgtcctcct tcatttattt
    10501 cgcgtctttc ggaatttcac acgccacaca cggtttcatg tagcacatca tatcgtggaa
    10561 ctgctctttc tccacgcacg gcggggtgag gttcgcgcac ccgccgcaga tcgctttctc
    10621 cggatctgtg tacgtgcacc gcaactggca ggccgggcac acgtacatgc acccgccgca
    10681 cagccggcac acctccgact tcacgtcgaa cggcgtgccg atcccgcggt gctcgccccg
    10741 gccgtagaac ccgatcgcct gggccaccat ctgctccttg cacatccgca cgcacagccc
    10801 gcagaggatg cagtcctcgt gctcctgccg gaagcgctgc tgccgcacct ggtgggccga
    10861 ggcgatgtcc tgaatcacct tcgactgcgg gcacgaggcg aggagcagct cgatgatcat
    10921 ccgccgcgcc cgcagcaccc gctccgatgc cgtccgcacc gtcagcccct cctgcaccgg
    10981 gtacgtgcac gacgtcacca gccgcgccgc cggcccctcg ccgatctcca ccacgcagag
    11041 gcggcacgcc ccgtagggcg tgagcccctc catgtggtac agcgtcggaa tcggaaaacc
    11101 gagaaactgc gccgcctcca ggattgtcga acccgtctcc acctggacgc gaagcccgtt
    11161 gattgccagt gtcgtcatag ctgcttacct ccggtgacgg cgaccgcgcc gtcgtgatcc
    11221 gtttcctgcc cggtctcggg cggccgcgtg aactcgaggt cgcaccgcag gcaccgccgc
    11281 gcctcgcagt gggcctcggc gaccgacagc gccacctcca cctcggcgaa attgcgcgtc
    11341 cgccaggccg ccggcgcccg cggcgtctcc acccgctcca ccgtcggaag gtcgtcgagc
    11401 tcggcgctca ccggcggcac gtacacccgc ggcagagccg gctcggccgc atccgccagc
    11461 tcgagcccgc gcacgtaccg gtcgatcatc cgtgccgccc gcttgccgtc ggcgatcgcg
    11521 tcgaccaccg tgttcgggcc gcgcaccacg tcgcccgccg cgaacacccc cggccgcgtc
    11581 gtcaaaagcg tcttcttgtc caccttcacc gtgttccagt cggtgatcgc gatcccgctc
    11641 tgcttcgccg ggccgatcgc gtcgaccccc gaatcctcgc tgatcgccac gatcagcgtc
    11701 tcgagatcca cccggaactc gctccccgcg atcggcaccg gccggtgccg ccccgactcg
    11761 tcgatctccc ccagccggtt gcgcagacac tccagcccga ttagcttccc gtctttcgcc
    11821 agcaccttct tcggcgtcac gagaatctgg atgtcgatcc cctcctgcgc ggccgcctcg
    11881 atctcctcgg cgaacgccgg catctcctcc cgcgtccgcc ggtagaggat ggtcacgctc
    11941 tcgacgtcgc gctggcggag ggccacccgc gccgcgtcga aggccgagtt gccgcccccg
    12001 atcaccccca cccggccgcg cgccctcgcc tcgccccgct ggttgaacgc cttgaggaat
    12061 tcgatcgacg ggtgcacccc cgcgcagtcc tcgttctcca gcttcagcgg cttgctttcg
    12121 tgcgcgccca gcgccagcag gaccgcccgg tagccctcgc cgagaaggcc gtcgaccgtg
    12181 atctccctgc cgagcgccat ccggcaccgg accgtgatat tgtcgttgag gaccgcttcg
    12241 atctcccggc ggatcacctc ctgcggcagc cggtatcccg gaatcgcgca gtagagcata
    12301 ccgcccggac ggtcttcctt ttcgaagacg gtcacgtggt aacctttgag ggacaggtag
    12361 tgggccgccg tcaacccggc cggccccgac ccgatcaccg ccaccttggg cgccggaccg
    12421 tccgtccaga cctcccgccg gggcgtgtag gccgaggggt cgatgcggtc cgtgatgaac
    12481 cgcttgagcg cccggatcgc caccgggttg gtccccgtct ggcccgcctt gcagcgctcc
    12541 tcgcacggat ggtggcagac gcggccgcac accgacggga acggattggc ctcccggatc
    12601 accgcgtagg cctccttgta ctcgccgttg gcgatgtgcg cgacataccg ccacgcctcc
    12661 gtcccgatcg ggcaggccga ctggcagggc gcccccacca gctccgtgca cacgaacgcg
    12721 tcgcagcgct tgtccaggat gtgccgctcg aactcctggc ggaaataccg gatcgtgctc
    12781 agcaccgggt ttggcgccga ctgccccaga ccgcacatgc tcgtgtcttt gaccaccagc
    12841 gccagctccg ccagaaggtc gaggtgctcc atcgtcgcct tccccttggt gatgtcgtcg
    12901 agaatctccc acatccgctg cgtccccttg cggcaggtga agcacttccc gcacgactcg
    12961 tccttgagga acttcatgaa atacttcgcg agatccacca tgcaggtgtt ctcgtccatg
    13021 acgatcatgc cgcccgaacc catgatcgaa ccggccttgg tcagactgtc gtagtcgatc
    13081 gagaggtcga acatcgaggc cgggatgcac ccgcccgaag gaccgcccgt ctgcaccgcc
    13141 ttgatcttcg ccttgcccgc cgccccgccc ccgatcccgt acaccacctc gctgatcgcc
    13201 gtccccagcg gcacctcgac cagcccggtg ttccggatct tccccaccag cgagaagatc
    13261 ttcgtcccgg tgttccccgg cagacccacc ttcgcgtact ccgcgccccc gcgggtgatg
    13321 atctccggga tgttggcgaa agtctcgacg ttgttgatgc aggtcgggct gccgaaaatc
    13381 ccccgctcga tcgggaacgg cggccgctgc cgcggctcgc ccatgtaccc ttcgatcgac
    13441 ttgatcagcg ccgtctcctc cccgcacacg aacgccccgg ccccgcgcac gatctcgata
    13501 tcgaaatcgt gctccgtccc gagaatcttc tccccgagaa ggccgaggtc gcgcgcctgc
    13561 cggatcgcga tgagacagtg cttgatcgcc agcggatact cgctgcgcac gtagatgatc
    13621 ccccgcgtcg ccccgatcgc cagccccccg atcaccatcc cctccatgat cgcgtgcgga
    13681 ttcccctcca gcacgccgcg gtccatgtac gctcccggat ccccctcgtc ggcgttgcac
    13741 acgatgtatt tctgctgccc cggccgcccc gaggtccgcg ccagcgccca cttccgcccg
    13801 gtcgggaaac cggccccccc gcgcccgcgg atccccgccg tctgcacctc ctggatgatc
    13861 cactccgggt cgttcttctc cagcgccttc agcagcgccc cgtacccgcc gttctcgaaa
    13921 taatccatga tccggatcgg gtcgagcttc tggttgttgc ccacgatcgt ccgcttctgc
    13981 cgcgcgaaaa acggaatgtc gctctgccgc gggtacactg tcccggtcgc cgcgtcgcgg
    14041 tagagcaggt cgtcggccgg ctgcccgctc agcgccgcct cgattatccg cggcacgtcc
    14101 gccggtctca gcctgggata gaagtgcccg tacggctcca ccgtcacgta ggggttcatc
    14161 tcgcagaacc cgtggcaccc cgtgatgcgc aggcgcagtt tttcctgaag gaaccggtcg
    14221 agcatgtgct tcttgaacag ccggatgagg tcgttcacgc cgctcgcctg ggcgcccgtg
    14281 tcggcgctca ccacgatcgt cggaatcgcc tccgcccgcc gccgctccgc catcacccga
    14341 ctccggaatc ccgccaggtc atcgactgat gtcagttttc ttgccattta tgccgtctcc
    14401 tcgttcatcg cattgcctcc gtcagtcggc cgccgtgccg ccgaaatcga ggatgtgtcc
    14461 cttgcagccc cgccgcgagc agagctgcac gataccgccg ccgcgcacga tcatcggcac
    14521 catcggcgcc ccgcactccg cgcagggcgc cccgcccatc atctccgcat ggcagtgcgg
    14581 acagaaaaac gtcaccaccg tctccgccgg aatctcgtgc tccgagtgca ccgtgtacga
    14641 tccccacagc gacgatatcg ccagccagct gtgcttgctg ccgaacgagg ccgtcacccg
    14701 taccgacggg tgcccctcga tctcgtgccg cggatccatc agactgtggt tgcaccgcgc
    14761 gcagctcacc tcgaccggga agatccgctc gtccgtctcc acccggactg cgtcgagccc
    14821 ctccttcgcc cgcgcgagaa tgtccgggat catcgatgtc ttcactttcg aaaagtagtg
    14881 cccgtcgacc accacgatcg gccccaacgc gcatgccccc aggcagttca ccgtctccag
    14941 cgtgaactcc cggtccgccg tcgtctcccc cgccctgatc cccagccgct tctcgatctc
    15001 ccgcgcaatc gccggccccc cccgcacgtg gcacgccgtc cccagacagc acgacaccag
    15061 gtgcttcccc cgcggcctca aactgaacgc ccggtaaaac gtcgccaccc cgtagatgtc
    15121 gaccagcgac cggcccgtct tctccgccac cacccggagc atcgcctccg gaaggtagcc
    15181 gtacttcgcc tgcatgtcct cgagtatcga aatgagactg ccgcgattcc cgtggtgctt
    15241 ctccacgatg ctgaggactt cctgtgtgtt catggcctgc ccttctgctt cacatcgatc
    15301 gtccgccgcc gcaggcgcgc ccggccgccc tgagcgcagt cgatgggcga ccccttcgcc
    15361 cgcccgccgc cgtcctccgg ccgcgggcag cttccgaccg acatcgatct atgttcatgt
    15421 gtattcctcg caatgccaga tccccgcctc aggacgcgga tgcgcacagg tggccgctcc
    15481 ggcgcaggtc gcacacaatc cgcgcgcccc cccgtcgccg ccggtatcgt tctcacgcgc
    15541 ggacggctga ccctccggtc cgtccccaaa tgcccggtgc tcgaacatct cgcagtacaa
    15601 cgcgtccacc ccccgcttct cccgaaagac acacccgcgg cggaacttgc acgtcgggca
    15661 cagccccgcc ggcgccgtcc gctcacccga ccacacaacc cgcccctccg ccctgtacgg
    15721 ggaaaaccgt cccacccgac catccctgtc gcactcccga catcgtcgca ttctgggtca
    15781 atttctccat acatcacacc ctcccctatc agattgcatg ccaagtcgct gtcgcgctcg
    15841 ctttctctga agacccgcac accaccgtcc aacccggcat cgtcgcactt gcttgattcg
    15901 catgctgata attgtcattt cgggaatcgc attcgacgat tcaaaaagtc tatattacca
    15961 ccgctcaacc actaaccgtt atcggggaat ttctccccac cggatcattt ttcctcgatg
    16021 atgacaggag atctcacgcc cgaggccgag cactccacgc cccctcctcc tccccgcccc
    16081 aaccctccaa aatccctccc accccatcaa ttctcgttga cacccggctc ctctgggggc
    16141 tattgattca tagattgcag ggccggccga tcttcaaggc gacgctttcc tgcgggtgcg
    16201 gacgacaccg gaaacggtcc gccgaccctt tggatggagg gactatgtcg gacaacgatt
    16261 catctctggc gggtcgcgcc gccgtggcca tggccctgac tatcggcttc tactcgctcg
    16321 cactcatcct cggcctcggc acgatcctgc tgccgttcat catcgcgtgg gccaccggcc
    16381 agttgattct gaaactggtc atcgtctgcc tcatctccgg cggcgcaatt ctggcggcca
    16441 tcgtcccgag gcgggaccag tttgaaccgc ccggtcccga actgagtctc gccgagcacc
    16501 cccggctggc cgaagttatc cgccgcgtgg ccggtcaaac ggagcaagcc atgccccgca
    16561 ccgtctacct gattcccgat ttcaacgcct gggtggccca gcgcggcggc tcgcaccttc
    16621 acggcggcga ccgcctcatg gggatcggcc ttcccctgct ccaccagttg accgtctcgc
    16681 agttcgaggc cgtgatcgcc cacgaattcg gccactatca ccacggcgac acgagactcg
    16741 gcccccgcat ctaccagacc cgggccgcca tcgtccgcac catcgccggc ctgcacgagc
    16801 acaattccat cctccaggcg cccttcctct ggtacggcag gatgttcctg cagatcaccc
    16861 acgccatttc ccgccgccag gaattcaacg ccgaccggct cgccgcccag gtggccggca
    16921 ctgccgccat gcgctccgcc ctcgagaaaa tcaaccgggg cggcatcgcc ttcgatgcct
    16981 actggcagag cgagttcgtg cccatgctcc ggtcccgccg ccttccgccg ctgcttgacg
    17041 gctttactct ctttctccag tgtgagccga tgcagacccg gatagacgcc tgcgccgagc
    17101 aggttctcgc ctccgaggcc accgacaagt acgacaccca cccctcgctg cgcgaacggc
    17161 tcgccgccct tgaggccctc cccgccggac ccgcgctctc cgatcgccgg cccgcgctcg
    17221 acctcctctc cgaccccgcc gaacaggagc gggaactcct ccgcgcgatc gtcgccgcag
    17281 gcgaattcga acggctcacc ccggtgggct gggacgaggt cgctcccatc cacgccgccc
    17341 gctggcaggc catgagccgc gagcgccggc acttcttctt cgccatcacc cccaaggacc
    17401 tcccgcggtt tgtccgggat cccaagccct tcgtgaaaga caggcccctg caggatctgc
    17461 tcgccggccc caatcaggcc caggcagcgg aggcggtcgc cgtcaccgtc gccctcgccc
    17521 tcgcctccct cctcgtccgc ctcggctgga cgctcgagag gccgatcggc agtaaccccc
    17581 gcatggtcct cggagccgcc gttatcgaac ccttcgacac cctcgatctc ctcaggtcgg
    17641 gacagctccc cgcgccggag tggctccgcc tctgccgcga caccggaatc ggcgaccacc
    17701 ccctcttccc gccctctcat aaagaggatg gcgcgcgggc cgaatgagcc cggtcccccg
    17761 cggggcggtc ccgccgcacg accgcgccgc cccggccgcg tcttttctag cgttgactcc
    17821 gctccccgcc ctccgtatga tgagggggac agtatcgacc gattgcccga tccaccctcc
    17881 ggccgcgccc caacggcggc cggcagaacg
//
LOCUS       DAOPOE010000067        17880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3768_length_17880_cov_5.904460, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000067 DAOPOE010000000
VERSION     DAOPOE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17880
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3768_length_17880_cov_5.904460"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2587
                     /locus_tag="PKM94_09540"
     CDS             <1..2587
                     /locus_tag="PKM94_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOD66921.1"
                     /translation="AGSPVLNFDEFARDRCFGHTFSNCFDTACCVVGAQLCFKVRATD
                     PGATDDFIRLGEGANSNFLYQISLSQLLAIHTAGADTVWNFGDTMTVCLDLGNLPYAG
                     PYNVSSMIAALQDGTLDFMMIDDTQIDYLELIVQRCCPSQQYCSISGAKFNDLDHDGK
                     QDPGEPGLPGWTINLFYQGGPLYASAVTDANGNYTFANIPCSTYTVSEVNQPGWVQTT
                     LPTAYTVTTAPGLPAVNISFGNWMCGGGNSGVPDTCCVKPPAKMLAWYPLDEAAPATA
                     LDLAGHDNNGTQLGSFGFAPGMVGGAYEFGPNVPNLDIVRVYNDPFVTIGPGDFSADA
                     WIHPRSFGANCAVFNQNVGNQPCVDPIFDNRQWFFGGSGLDGVCFFVEDDGQGTGTAF
                     LGLIMSSFPAPQTVFYSPPGTVQLNQWQHVAVTVSRTTGGGTLYLNGAPVATFTPLTG
                     PLYSTNSGPILDIGHGTFLNTGGCYFTNRYFDGFLDEIEIFNRALTPAEVQGLYEAGA
                     TGKCKVRCRVPTVVFCKNQTTRPLAFTVCNDGAASAALNWSLAGMASGSGCNFNGAGL
                     VFTPASGLTAPLAPGACTTITVTVTRPAGFNPGAVACYSLSVLNPMNGSSSTCEGQIK
                     AGFGKWCATIIGLPIDPIGIGLGGGSGTARVMVTNDDDPTGSLSYGLQVLDHDGAPSQ
                     AVSLNGLQPGEPVLGNRMIPLGTTQEFEVQLTALLWPGPGPENLVLSIDEDGDGVLDR
                     TADVGVEVISFADCNGNGADDAQDILSGVSTDANGNGVPDECEAVCTEAYCRCPVCGD
                     VNGDGQVKVSDLTLFVSYLFRGGSAPVPLAVADVNGDGQVKVSDLTLFVSYLFRGGPP
                     PTCGF"
     gene            complement(2545..3660)
                     /locus_tag="PKM94_09545"
     CDS             complement(2545..3660)
                     /locus_tag="PKM94_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD66922.1"
                     /translation="MSFVSRTVALGAFALLWRALTQPLYDAGIGVFPCRGNHDGGSLA
                     PSHTVFPGPYALPANGPENEVNLTFAFTQRNALVIGLDQVLIRNRINLPWLQAQLDTT
                     AAEHIFVFGHMPAFKVDHDDCLDDYPGERDTFWNSLAAAGARVYFCGHDHFYDHARLA
                     DSDSDSADDLHQYVVGTAGAPLYAGGAYDGANGIWTPMLVHHEKEYGYVLVELDGPTA
                     MLTWKHRTAPGVYEDGGDRWPYSVPTAGCCGGDERRGDIDGRCLPECITVNDLAQFIA
                     YMFAGGRPPACAAEADIDASGGLDVADLTALLGFVYRGGAPRQPCRQARPGCRARRRP
                     RQSRARITGRGFLPQGVNSIPPGDQKPQVGGGPPRKR"
     gene            3834..5513
                     /gene="ettA"
                     /locus_tag="PKM94_09550"
     CDS             3834..5513
                     /gene="ettA"
                     /locus_tag="PKM94_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOD66923.1"
                     /translation="MAEKYIFHMYGVNKFYGQKQVLRDINLSFYPGAKIGIVGENGAG
                     KTTVLNIMAGRDTEFIGHAFITPGYRAGMVEQEPQLDPQLTVRQTIESAFGETKALLD
                     EYNRLADSMAAPADDAAMQKAMDRMGVLQDKIEAVDAWNLDQRLKVAADALCLPEDDR
                     IVGTLSGGERRRVALCKVLLEKPDLLLLDEPTNHLDAETINWLEEQLREYPGTVIIVT
                     HDRYFLDNVTRWILELDGGHGIPWEGNYSSWLEQKLAALAESEKKNSPRARALERELS
                     WIRMSRGDRTELTRARMREYEQLVARETAAAKGAEAVIRIAPGPELGSQVIEFREVGM
                     GYDSRRLFENLSFAVPRSAVVGLVGPNGAGKTTLLKMVTGDVAPSEGQVVIGSTVRLA
                     YVDQEREALAGGRSLIEEIGGGAEEIMIGKRAVPIRQYLAQFGFKGAAQQKMASELSG
                     GERNRCLLAKVLKSGGNVLLLDEPTNDLDVNTLRMLEEAILDFSGCVLVISHDRFFLD
                     RIGTHLLVFEGEGKVRWFEGTFSEYEEWRIREMGDRLFENRRNRYRTIVRA"
     gene            5604..6413
                     /locus_tag="PKM94_09555"
     CDS             5604..6413
                     /locus_tag="PKM94_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOD66924.1"
                     /translation="MPQIRATRLEKGEGRAETWLSRYLPYGTRRRKIALWAGLPLAIL
                     LVLYFIMDNIVMMQVTRHGEEFTLPNFEGQTMIEASVTLDNLGLSGEVASYEFAPGTP
                     KGVIINQFPRAATKVKDGRRIKFVVSAGQKMITIPSLAGESVRQAMLDLETAGLKLGE
                     IAWAFSDTLPERVVVFSYPAAGTEIPLGAPVNLMVNRGRASSFTYVPHVVGLTLAEAQ
                     KRIEDKNLRVGVVTYKKDDAYLPETVLEQSDPPGAELDIHTEIDLVVSKTD"
     gene            6472..7332
                     /locus_tag="PKM94_09560"
     CDS             6472..7332
                     /locus_tag="PKM94_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD66925.1"
                     /translation="MPDRLLISSAPVSLTRPMQAMVAKLYSPALLPAVALERITYLSD
                     SLKVSGYLARPAAEGRYPVVIWNRGGYREHGALDDFRAMLILGSTAQWGYVVLATQYR
                     GNAGSEGTESWGDKDVDDALNLIPLAETLPFCDPRRIAIEGASRGGMTTYRALARESR
                     FRCAIVHAGLADLFALAELRGQMRSWLDELFGDLSPAEKERELGRRSAVYFADRFPDH
                     VPLLMLHGSADTRVPLSQSEALAAELARFGKPHRLVVIPGGGHVALKDGSYREVDRHR
                     KAWLAQHLVG"
     gene            7650..8975
                     /gene="ffh"
                     /locus_tag="PKM94_09565"
     CDS             7650..8975
                     /gene="ffh"
                     /locus_tag="PKM94_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOD66926.1"
                     /translation="MFSALSDKLEDVFKKLRGAGRLSEKNIRDAMREVRQALLEADVN
                     YKVAKRFVAAVEAKAVGEEVLRSIEPGQQVIKIVHDELVLLLGGKAEPLAPLDRAPTI
                     FMVCGLQGSGKTTLCGKIALWAKRKNKKPLVVAADIYRPAAVKQLRVLAESIQVPHFS
                     LDGAAPPEICREALRHAERSFIDLVILDTAGRLHVDEEMMVELQQIKEQVRPHEILLV
                     ADAMTGQDAVNVAREFHDRLAITGVVLSKLDGDARGGAALSIREVTGTPIKLASVGEK
                     LGDLEPFHPDRLASRILGMGDIVTLVERAQEHIDQAQAEKMQQKLLRAKFDFEDFLEQ
                     MEQIKKMGPLDSLLQMIPGVGKALKGVSVEPKEMERMAAIIKSMTIEERRNPGMIDGS
                     RKKRIAAGSGNSVQTINQLLKQFTAMQKMFHNMNKGNLKGIPKGMLPFG"
     gene            9000..9410
                     /gene="rpsP"
                     /locus_tag="PKM94_09570"
     CDS             9000..9410
                     /gene="rpsP"
                     /locus_tag="PKM94_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014974045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOD66927.1"
                     /translation="MAVHIRLLRMGKKKQPFYRIVAADSRRARGGRFLEVLGTYNPIK
                     KPAAVSLLEDRVTKWLDDGAVPSDTVRSLLTQTGMMEKYARAKAGQDVSAIQIRPTIT
                     ERRKRTRRMKKATVAAAADAPKAEAAPPAGEKTE"
     gene            9504..9734
                     /locus_tag="PKM94_09575"
     CDS             9504..9734
                     /locus_tag="PKM94_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD66928.1"
                     /translation="MKEFVEYIVKSVVDNPDDVEVSEVQGSRTTVYELRVAQADLGKV
                     IGKAGQTAKAIRTLLAAAAARRGKRAVLEILE"
     gene            9747..10289
                     /gene="rimM"
                     /locus_tag="PKM94_09580"
     CDS             9747..10289
                     /gene="rimM"
                     /locus_tag="PKM94_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOD66929.1"
                     /translation="MEYVRVGWLGRTRGVGGDVWVTPDTDFPERFLGLKEIFVRNRDQ
                     WERLAIAHALLIGGRPVLRFAGISSPEEARRLTNRELAVARDRVCELPAGSHYIFELV
                     GCAVYDEAAVLIGELVDVESYPANDVYVIRTPEGGRKCLPAARPFVKDIDTAGKRIVI
                     DPAGLFDAAGAAPRDTGNEI"
     gene            10279..11082
                     /gene="trmD"
                     /locus_tag="PKM94_09585"
     CDS             10279..11082
                     /gene="trmD"
                     /locus_tag="PKM94_09585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD66930.1"
                     /translation="MKFELITLFPDYFRLALGQSLLGKARERRLFDIEVINLRDFATD
                     RHRTVDDTPFGGGGGMLLKVEPLDRCLASLGYRRRGQDGPPHERERIILTSAAGATFR
                     QDAAVRYSLCERVTIICGHYLGVDERILQLYEVDEVSIGDYVLTGGEPAAAVLVDAVA
                     RLIPKVLGNFESALEDSYMNQLLGTPSYTRPAVYEGLAVPEPLLSGNHAEIRDYRRRR
                     ALERALERRPDLLAAAELTDEERAWIARRRREQGAPPADSAGAADENEM"
     gene            11116..11448
                     /gene="rplS"
                     /locus_tag="PKM94_09590"
                     /pseudo
     CDS             11116..11448
                     /gene="rplS"
                     /locus_tag="PKM94_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            11647..12027
                     /locus_tag="PKM94_09595"
     CDS             11647..12027
                     /locus_tag="PKM94_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66931.1"
                     /translation="MASEVIKVCPCCGEPFTLQDIVFRRDILPIGMLLEDDDLRHNFY
                     YFNHLAPSCGTTFTIPVELLRAQIVGEIPSERLSGTARCGRHCLDLADTGPCDRECAY
                     APYRRLLQYLKDVRRADRASTPRI"
     gene            complement(12082..12660)
                     /locus_tag="PKM94_09600"
     CDS             complement(12082..12660)
                     /locus_tag="PKM94_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOD66932.1"
                     /translation="MRRRKQRQFEFTIVAGDLKGRRITAPDLGSTRPPLSRVRKAIFD
                     FLTPHLEGAAYLDLFSGTGSYLFEAVSRGAAFALGVEQERLLADAINRQAAALGVADR
                     LRCLRDDVFRTIAHLSDDSRRWPVIMLAPPQYRGMIDRTLAALREHPLSAPGGLLICQ
                     HDSSETGRIDFLDFSIQQRRAYGNTTFTVVEA"
     gene            12793..13455
                     /locus_tag="PKM94_09605"
     CDS             12793..13455
                     /locus_tag="PKM94_09605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOD66933.1"
                     /translation="MADLMTTHMRKTASLPIDDVAGMLANLGFRTMCGVDEAGRGPLA
                     GPVVAAAVIVPHGFALDGLTDSKLMTPAQRDEVFARIAEAGFPCAVGIIDHETIDRVN
                     ILKASLLAMRRAVMELPAPPECVIVDGNHTIPNLAFPQYALVHGDRLCPAVSAASVIA
                     KVTRDRIMDRMERLYPQFRFSQHKGYCTPAHLEELRAHGPCEIHRKSFGPVAELLQYA
                     LV"
     gene            13442..13825
                     /locus_tag="PKM94_09610"
     CDS             13442..13825
                     /locus_tag="PKM94_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOD66934.1"
                     /translation="MPSSKRPRGKGTGRVAAGRRYERLAADFFRANGFEVRAQNFRAG
                     AREIDLIVQRDRLVVFVEVKAARDESFGHPAERVDGRKREHLVAAARRYMGENDLSGC
                     DLRFDCITFVGGQIEHYPNAFLVEE"
     gene            complement(13836..14228)
                     /locus_tag="PKM94_09615"
     CDS             complement(13836..14228)
                     /locus_tag="PKM94_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66935.1"
                     /translation="MVWFRRLLALALVLLAVGGYAYVRQHRRAAAQAERAHLALLTAR
                     IWVGTAEYRDRPAVFLAWRDSLLAAENLTADSLRVRVDRLRADPSASGDYFRQVSRFV
                     DSLVRIRTAPAETSAGSAPPPGVRVRRE"
     gene            14309..15400
                     /gene="ispG"
                     /locus_tag="PKM94_09620"
                     /gene_synonym="gcpE"
     CDS             14309..15400
                     /gene="ispG"
                     /locus_tag="PKM94_09620"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOD66936.1"
                     /translation="MELTYPIRRRKSKAVKIGSVIIGGGFPVAIQSMTTADTRNVEAT
                     VAQTLDLFAAGCDIVRLSVLNHEAADCLPEIRRRVPDRPLVADIHFQYKLALKAIAAG
                     FDKIRINPGNIGAEWKVREVTRAARDAGVPIRIGVNSGSLPKDLLETYGHDDPRAFTE
                     AALREIAVLQDMGCADIVISVKSTNAETAFWAYHMLAAQTEYPLHVGITEAGTLETGS
                     LKSAVGIGAILMTGIGDTIRVSLSADPIHEPRVARQILTACNLKNEGVQVIACPTCGR
                     CQIDMIPLAESVERKTRHIKTPLKVAVMGCAVNGPGEAAAADVGIAGGDGMGMIIRKG
                     QIIKRFPEAELEQRLLDEIEELTGEKIPR"
     gene            complement(15427..16560)
                     /locus_tag="PKM94_09625"
     CDS             complement(15427..16560)
                     /locus_tag="PKM94_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66937.1"
                     /translation="MRHFVVFGLLPLVILGCGATVPRYEVWRPWTRVLGEGQAVPIGQ
                     SIQIRVEGTTDPMPGHEELLQDQLASIAGELLARRGYQIVESNPDLMLTLRYRTERVE
                     EFAAAASASVTAETASSVGMRFRSASVSDVGLGVAVASAVVQRLTRTSSSAAVAIETT
                     TGFVHTIELALAPPRQQPVWTGESRWQTEDVEIVPGVKDALRLILSRLPSDSALIPAV
                     PALRDSHIKNFLDAYCGETPYSCPALPYRIFLRFVWPPQYVQDGMGSYGFRASGLLLD
                     PAALPAYLDLAQTAEFALPIGKGNYSKPLDLNLWKEVMLGGRYRLGPDSQLVNVLLEL
                     EGRAEGYDVKKAWLASDSEFAEFRDRLRRWQEVLRVFFDMYED"
     gene            16849..17868
                     /locus_tag="PKM94_09630"
     CDS             16849..17868
                     /locus_tag="PKM94_09630"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOD66938.1"
                     /translation="MNRILIALLLCLAATPPSPTANVKPRVSLQPSLRYGFGETEYVL
                     DARWYDDEGVLQHLKSQLEFPLDGLYAGLEGRVRWNPASTKPWDSKLSVSTNLSDPAG
                     DMTDRDWSTFWESGSETFYLPSFTELSSTVSSAESRSWVVAAEVGKVLAAGPKGSIRL
                     FAGYRYQHIAQKAIGISGWQMVNVGGELVKGYFDGSRDGTTFIDYEVTFHSPQAGLGL
                     SLAVTDRAAVAARAAYAPVFASDRDDHVVRNFLSEASINGHGVIGEAEVEWELGRGDS
                     WGLFLSAGGEFLYFSASGTGTQFYYGDDPGTRNEDETGQYAAGVPHEIHTTQGRVGLG
                     LALRF"
BASE COUNT         3140 a         5789 c         6082 g         2869 t
ORIGIN      
        1 cgcggggtcg ccggtgctga atttcgacga gtttgcgcgc gaccgctgtt tcggccacac
       61 cttcagcaac tgtttcgaca cggcctgctg tgtggtcggg gcgcagttgt gtttcaaggt
      121 gcgcgccacc gacccggggg cgaccgatga cttcatccgg ctcggcgagg gagccaattc
      181 caacttcctc taccagatca gcctgagcca actgctggcg attcacacgg ccggggccga
      241 cacggtctgg aatttcggcg acaccatgac ggtctgcctc gatctcggca acctgcccta
      301 cgccgggcct tacaacgtct cgagcatgat cgcggcgctc caggacggca cgctcgactt
      361 catgatgatc gacgacacgc agatcgacta cctggagctg attgtgcagc ggtgctgccc
      421 gtcgcagcag tactgcagca tcagcggcgc caagttcaac gatctcgatc acgacggcaa
      481 gcaggatccg ggcgaaccgg ggctgccggg ctggacgatc aacctgttct accagggcgg
      541 gccgctctac gcgtccgccg tcaccgacgc caacggcaac tacaccttcg ccaacatccc
      601 ctgctcgacc tacacggtca gcgaggtgaa ccagccggga tgggtgcaga cgacactgcc
      661 gaccgcctac acggtgacga cggcgccggg cctgccggcg gtgaatatca gtttcggcaa
      721 ctggatgtgc gggggcggca acagcggcgt tcccgacacg tgctgcgtga agccgccggc
      781 gaagatgctg gcctggtacc cgctcgatga ggcggcgccg gcgaccgcgc tcgacctggc
      841 cggccacgac aacaacggca cgcagctcgg gagtttcggt ttcgcgccgg gcatggtcgg
      901 cggggcgtat gagttcgggc cgaatgtccc caatctcgac atcgtgcggg tctacaatga
      961 tccgttcgta acgatcgggc cgggtgattt cagcgccgat gcctggatcc acccgcggag
     1021 cttcggcgcc aactgcgcgg tgttcaatca gaatgtcggc aaccaaccct gcgtcgaccc
     1081 catctttgac aaccgccagt ggttcttcgg cggctcgggt ctcgacggcg tctgcttctt
     1141 tgtcgaggac gatggccaag ggacggggac ggctttcctc ggcctcatca tgagcagttt
     1201 tccggcgccg cagacggtgt tctacagccc gcccggaacc gtgcagctca accagtggca
     1261 gcacgtggcg gtgacggtgt cgcggacaac cggcggcggg acgctctacc tcaacggcgc
     1321 gccggtggcg accttcacgc cgctcaccgg gccgctctac agcaccaaca gcgggccgat
     1381 cctggatatc gggcacggca cgttcctgaa caccggcggc tgctatttca cgaaccggta
     1441 cttcgacggt ttcctcgatg aaatcgagat tttcaaccgg gcgctcactc cggccgaggt
     1501 ccagggtcta tatgaggcgg gagccacggg caaatgcaaa gtgcgctgcc gcgtgcccac
     1561 cgtggtcttc tgcaagaacc agaccacccg gccgctggcg ttcacggtgt gcaacgacgg
     1621 cgccgcgagc gcggctctga actggtcgct ggccgggatg gcgtcggggt cgggctgcaa
     1681 tttcaacggc gccggcctgg tgttcacgcc cgcgagcggg ctcaccgcgc cgcttgcccc
     1741 gggcgcctgc acgacgatca cggtgacggt gacccggccg gccggattca accccggcgc
     1801 cgtggcctgc tacagcctga gcgtgctcaa cccgatgaac ggctcgtcct cgacctgcga
     1861 agggcagatc aaggccgggt tcggcaaatg gtgcgccacc atcatcggcc tcccgatcga
     1921 cccgatcggc atcggcctcg gcgggggaag cggcactgcc cgcgtcatgg tgaccaacga
     1981 cgatgacccg accggatcgc tctcctacgg tctccaggtg ctcgatcacg acggcgcgcc
     2041 gtcgcaggcc gtcagcctca acggtctgca gccgggcgaa ccggtcctgg gcaaccggat
     2101 gattccgctc gggacgacgc aggaattcga ggtccagctc acggccctcc tttggccggg
     2161 cccgggtccc gaaaacctcg tgctgagcat tgatgaggac ggcgacggag tactggatcg
     2221 gacagccgac gtcggggtgg aagtgatctc gttcgccgac tgcaacggca acggcgcgga
     2281 tgacgctcag gatattctga gcggtgtctc caccgacgcc aacggcaacg gcgttcccga
     2341 cgagtgcgag gccgtgtgca cggaggcgta ctgccgctgc ccggtgtgcg gcgacgtgaa
     2401 cggcgacggg caggtgaagg tgtccgacct gaccctgttc gtgtcgtatc tgttccgcgg
     2461 cggatccgcg ccggttccgc tcgcggtggc cgacgtgaac ggtgacgggc aggtgaaggt
     2521 atctgacctg accctgttcg tgtcctacct cttccgcggc ggtccgccgc cgacctgcgg
     2581 tttctgatcc cccggtggga tagaattgac gccttggggc aaaaagccgc gtccggtgat
     2641 ccgggcgcgg ctttgccttg gtcgacgccg ggcgcggcag ccggggcgcg cctggcggca
     2701 gggctgccgc ggcgcgccgc cgcggtatac gaaaccaagg agggcggtga gatcggccac
     2761 gtcgaggccg ccggaagcat cgatgtcggc ctcggccgcg cacgccggcg gccggccgcc
     2821 ggcgaacatg tacgcgatga attgggcgag gtcgttgacg gtgatgcact ccggcaaaca
     2881 ccgaccgtcg atatcgccgc gccgctcgtc gcccccgcag catccggccg tcggcaccga
     2941 gtacggccac cggtcgccgc cgtcctcgta gacgccgggg gcggtccggt gtttccacgt
     3001 cagcatcgcc gtcggaccgt cgagctccac cagcacgtag ccatactcct tctcgtggtg
     3061 cacgagcatc ggagtccaga tcccgttggc gccgtcgtac gccccgccgg cgtagagcgg
     3121 cgctccggcg gtgccgacta catactggtg aaggtcgtcg gcggaatcgg agtcgctgtc
     3181 ggccagacgg gcgtgatcgt agaagtggtc gtggccgcag aaatagacgc gcgccccggc
     3241 ggccgccagg ctgttccaga acgtatcgcg ctcgcctggg tagtcatcca ggcaatcgtc
     3301 gtgatcaacc ttgaaggcag gcatgtgacc gaacacgaag atgtgctccg ccgcggttgt
     3361 gtccaactgc gcctgcagcc agggcaggtt gatccggttc cgaatgagca cctggtccag
     3421 gccgatgacc agggcattgc gttgggtgaa ggcgaaggtg agattcacct cgttctccgg
     3481 tccgttggcg ggcagcgcat agggaccggg gaacacagtg tgcgagggag caaggctgcc
     3541 cccgtcgtgg tttccccggc acgggaacac cccgatgccg gcatcgtaga gaggctgggt
     3601 cagagcgcgc cagaggaggg cgaaggctcc gagcgcaacg gttctgctga cgaatgacat
     3661 cttgctgctc ctgccgaaag agggctgtct gttgggatct ccgccgccat gtgccggatt
     3721 tgctgcgggg agtcaacaac cctctcgccc gcccgtttag gctcttgcgc cgcctgcaac
     3781 ggtgtgttac catggcactt gccacactgt cgatgggaac ggagaagaat cgtatggccg
     3841 agaaatacat cttccacatg tacggcgtca acaagttcta cgggcagaag caggtgctcc
     3901 gcgacatcaa cctcagcttc tacccggggg ccaagatcgg gatcgtcggc gagaacggcg
     3961 ccggaaaaac caccgtactg aacatcatgg ccggccgcga caccgagttc atcgggcacg
     4021 cttttatcac acccggctac cgggccggga tggtcgagca ggagccgcag ctcgatcccc
     4081 aactgactgt ccgccagacc atcgagtcgg ccttcgggga gaccaaggct ctcctggacg
     4141 agtacaaccg gctggccgac agcatggcgg cgccggccga cgacgcggcg atgcagaagg
     4201 cgatggaccg gatgggcgtg ctgcaggaca aaatcgaggc cgtcgacgcc tggaatctcg
     4261 accagcggct caaggtggcg gccgatgcgc tgtgtctgcc cgaggacgac cggattgtcg
     4321 gcacgctgtc gggcggcgaa cggcggcggg tggcgctgtg caaggtgctc ctggaaaaac
     4381 ccgacctgct gctgctcgac gagccgacca accacctcga cgccgagacg atcaactggc
     4441 tggaggagca actgcgggaa tacccgggaa ccgtgatcat cgtgacgcac gaccggtact
     4501 ttctcgacaa cgtgacccgg tggattctcg agttggacgg cgggcacggg atcccgtggg
     4561 aggggaacta cagttcctgg ctggagcaga aacttgcggc gctggccgaa tcggagaaga
     4621 agaattcgcc gcgggcccgg gcgctggagc gggagttgtc gtggattcgg atgagccggg
     4681 gggaccgcac cgagctgacc cgcgcgcgca tgcgcgagta tgagcaactg gtggcgcgcg
     4741 aaacggcggc cgccaaaggg gccgaggcgg tcatccgcat cgcccccggc cccgaactgg
     4801 gaagccaggt gatcgagttc cgcgaggtcg gcatgggata cgacagccgg cgcctttttg
     4861 agaacctctc gttcgccgtc ccccgcagcg ccgtggtcgg gctggtcgga ccgaacggcg
     4921 ccggcaaaac gacgctgctg aagatggtca ccggtgacgt ggcgccgtcg gaaggacagg
     4981 tggtgatcgg ctcgacggtc cggttggcgt atgtcgatca ggagcgtgag gcgctcgcgg
     5041 gcggccggag cctgatcgag gaaatcggcg gcggggccga ggagatcatg atcggcaagc
     5101 gggcggtgcc gattcggcag tacctcgccc agttcggatt caagggcgcc gcccagcaga
     5161 agatggcgag cgagctgtcc ggcggcgagc gcaaccgttg cctcctggcg aaagtgctca
     5221 aatccggcgg caacgtcctg ctgttggacg agccgaccaa cgatctggac gtcaacaccc
     5281 ttcgcatgct cgaggaggcg atcctcgatt tctccggctg cgtgctcgtc atcagccacg
     5341 accggttctt cctcgaccgc atcggcaccc acctcctcgt gttcgagggc gaagggaagg
     5401 tgcgctggtt cgaggggact ttctccgagt acgaggagtg gcgaatccgg gagatgggcg
     5461 accgcctgtt cgagaaccgg cggaaccggt accggacaat cgtgcgggcc tgagtgcggg
     5521 ccgcggtgcg ggcggcgttt taaccttgcg cccggcatga gaattcccca ccctgccgga
     5581 gtataaggaa tcggaaaaag agtatgccgc agattcgcgc cacccgactg gagaagggag
     5641 aaggccgggc cgagacctgg ctgagccggt atctcccgta cggcacccgc cggcggaaga
     5701 tcgctctctg ggcgggtctc ccgctggcca tcctcctggt gctctatttc atcatggaca
     5761 atatcgtgat gatgcaggtg acccgccacg gcgaggaatt cacgctgccc aatttcgagg
     5821 ggcagaccat gatcgaggcc tcggtcacgc tggacaacct cggtctcagc ggcgaggtgg
     5881 cctcctacga gtttgcgccg gggaccccca agggggtgat catcaaccag ttcccgcggg
     5941 ccgcgaccaa ggtcaaggac ggccggcgga tcaagtttgt cgtctcggcc ggccagaaga
     6001 tgatcaccat cccgtcgctc gcgggcgagt cggtgcggca ggcgatgctg gatctggaga
     6061 cggcggggct gaagctgggc gaaatcgcct gggccttctc cgacaccctg ccggagcgcg
     6121 tcgtcgtgtt ttcgtacccg gcggccggga ccgaaatccc gctcggcgcg cccgtgaacc
     6181 tgatggtcaa ccgggggcgg gcctccagtt tcacctatgt gccgcacgtg gtcgggctga
     6241 cgctcgccga ggcacagaag cggatcgagg acaagaacct ccgggtcggg gtggtcacgt
     6301 acaagaagga cgacgcctat cttccggaga ccgtgctgga gcaatcggac ccgccggggg
     6361 cggaactgga cattcacacc gagatcgatt tggtggtgag caagacggac tgaggggcgg
     6421 ggacccgccg ccatctcgac cgcagatctc acgattctca cggcacccgc gatgcccgat
     6481 aggcttctca tctcctccgc cccggtgtcg ctgactcgcc cgatgcaggc gatggtcgcg
     6541 aaactgtatt cccccgcgtt gctccccgcg gtcgcgctcg aacgaatcac ctatctctcc
     6601 gacagtctga aagtcagcgg ctacctcgcg cggccggcgg cggaggggcg gtacccggtc
     6661 gtgatctgga accgcggcgg ctaccgggag cacggggcgc tcgatgattt tcgcgccatg
     6721 ctcatcctcg gctcgaccgc ccagtggggg tatgtcgtgc tggcgacgca gtaccggggg
     6781 aacgccggtt cggaggggac cgagagctgg ggggataagg atgtcgacga cgcgctcaat
     6841 ctgattccgc tggccgagac gctccccttc tgcgacccgc gccggatcgc tatcgaaggt
     6901 gcgagccgcg gcgggatgac gacctatcgg gcgctcgcgc gcgaatcccg ctttcgctgc
     6961 gcgatcgtgc acgccggact ggcggatctc tttgcgctgg ccgaactccg ggggcagatg
     7021 cgctcgtggc tggacgagct gttcggtgac ctctccccgg ccgagaaaga gcgcgaactg
     7081 gggcggcggt cggcggtgta ttttgcggac cggtttcccg accacgtgcc gctgctcatg
     7141 cttcacggca gcgcggacac gcgcgtgccg ctgtcgcagt cggaggcgct ggccgccgaa
     7201 ctggcgcggt tcggcaagcc gcaccggctg gtggtgatcc ccggcggagg gcatgtggcg
     7261 ctcaaggacg gctcctatcg ggaggtggat cgccatcgga aagcgtggtt ggcgcagcac
     7321 ctggtcgggt agggatcggg cgacggggcc ccggcccgcc gccgtaaact tacggaacct
     7381 aaatcctctt ggtgcagcga tagttacggg tgcagcagga gagcctcccc gaagccgcca
     7441 ttcgtataaa cttcttgcgg tacaaacggt tttggttata ttgttgagct ttgcgccgac
     7501 cggcggttga atgtggacca gtcggtcgga ccgaagtggt gtgtcttttt ccgcccggcc
     7561 gcggcgttgt tgtcgacggt tggaggaaga cggcatggag actctggtgc agctcgcgtg
     7621 cggagcgggc gttttgatgt ttcggtagta tgtttagcgc gttaagcgac aagctcgaag
     7681 acgtcttcaa gaaacttcgc ggcgccggcc ggttgtcgga gaagaacatt cgcgacgcca
     7741 tgcgcgaggt gcgccaggcg ctgctcgagg cggacgtcaa ctacaaggtc gccaagcgct
     7801 ttgtcgccgc cgtcgaggcc aaggcggtcg gcgaggaagt tctccggtcg atcgaaccgg
     7861 gccagcaggt gatcaagatc gtccacgacg agctggtcct cctgctcggc ggcaaggcgg
     7921 aaccgctcgc gccgctcgac cgcgcgccca cgatcttcat ggtgtgcggg ctccagggct
     7981 cgggaaagac gacgctctgc gggaagatcg ctctctgggc gaagcggaag aacaagaagc
     8041 cgctcgtggt ggcggccgac atctaccgcc cggccgcggt caagcagctg cgcgtgctgg
     8101 ccgagtcgat tcaggtgccg cacttttcgc tcgatggcgc ggcgccgccg gaaatctgcc
     8161 gcgaggcgct ccgccatgcc gagcgcagtt ttatcgacct cgtcatcctc gacaccgccg
     8221 gccgtctgca cgtcgacgag gagatgatgg tcgagctgca gcagatcaag gagcaggtgc
     8281 gcccgcacga gattctcctt gtggccgacg cgatgacggg gcaggacgcg gtcaatgtcg
     8341 cccgcgagtt ccacgaccgg ctggcgatca ccggggtggt gctgtcgaag ctcgacggcg
     8401 atgcgcgggg cggggcggcc ctgtcgatcc gcgaggtgac cggcacgccc atcaagctcg
     8461 cctcggtcgg cgaaaaactc ggcgacctcg agcccttcca tcccgaccgg ctcgcctccc
     8521 gcatcctcgg catgggcgac atcgtcaccc tcgtcgaacg ggcgcaggag cacatcgacc
     8581 aggcgcaggc cgagaagatg cagcagaagc tgctccgggc caaattcgat ttcgaggatt
     8641 tcctcgagca gatggagcag atcaagaaga tggggccgct cgactcgctc ctgcagatga
     8701 tccccggggt gggaaaagcg ctcaaggggg tgtcggttga gccgaaggag atggagcgga
     8761 tggcggcgat catcaagtcg atgacgatcg aggagcggcg caacccgggg atgatcgacg
     8821 ggtcgcgcaa gaagcggatc gccgccggct cggggaattc ggtgcagacg atcaaccagc
     8881 tgctcaagca gtttacggcg atgcagaaga tgtttcacaa tatgaataaa ggaaatctga
     8941 aagggatccc gaaggggatg cttcctttcg gataaggccg tgagaaggag gaaggatttt
     9001 tggccgttca cattagactg cttcggatgg gcaagaagaa acagcccttc tatcggatcg
     9061 tcgcggcgga ttcccgccgg gcgcgcggcg ggcgctttct ggaagtgctc ggcacctaca
     9121 acccgatcaa gaagccggcc gcggtgagtc tgctcgagga ccgggtcacc aagtggctgg
     9181 acgacggcgc cgttccctcg gatacggtgc gctcgttgtt gacccagacg gggatgatgg
     9241 agaagtacgc cagggcgaaa gccggccagg acgtcagcgc gatccagatc cgcccgacca
     9301 tcaccgagcg ccgcaagcgc actcggcgga tgaagaaggc gaccgtcgcg gcggcggccg
     9361 acgcgcccaa agccgaggcc gctccgcccg cgggagagaa aaccgaatag cgcgaccgtc
     9421 cggcgcggac cagccaacgg aacttagcct gaaccgctta cgcgaactgc gatttcggag
     9481 acggaaaaag gaaggaccca gccatgaagg aattcgtcga gtacatcgtc aagtcggtgg
     9541 tggacaatcc ggatgacgtg gaagtcagcg aggtccaggg aagccgcacc acggtctacg
     9601 aacttcgggt ggcccaggcg gatctgggga aagtgatcgg caaggccggg cagacggcca
     9661 aggcgattcg cacgctcctg gcggccgccg ccgcgcggcg gggcaaacgg gcggtgctgg
     9721 aaatcctcga gtagccggag tccgcagtgg agtacgtgcg cgttggctgg ctgggccgca
     9781 cccgcggcgt cggcggcgat gtctgggtga cccccgacac cgattttccc gagcggtttc
     9841 tcggcctgaa agagattttc gtccggaacc gcgaccagtg ggaacgcctc gccatcgccc
     9901 acgccctcct catcggcggc cggcccgtcc tccgcttcgc ggggatctcc tcgcccgagg
     9961 aggcgcgccg gctgaccaac cgggaactgg cggtcgcccg cgaccgggtg tgcgagctgc
    10021 cggcgggcag ccactacatc ttcgagttgg tcggctgcgc ggtgtacgac gaggccgcgg
    10081 tgctcatcgg cgagctggtg gacgtggagt cgtacccggc caacgacgtg tacgtgatcc
    10141 ggactccgga gggcggccgc aaatgcctgc cggcggcgcg gccgttcgta aaggatattg
    10201 acacggccgg caagcggatc gtgatcgacc ccgccggcct gttcgacgcg gccggcgcgg
    10261 cgccgcgcga caccggcaat gaaatttgaa ctgatcacgc tcttcccgga ctacttccgc
    10321 ctggccctcg ggcagtcgct tctgggaaaa gcgcgcgagc ggcggctctt tgacatcgag
    10381 gtgatcaacc tgcgcgactt cgccaccgac cggcaccgca ctgtcgacga cacccccttc
    10441 ggcgggggcg gcggcatgct cctgaaagtc gagccgctcg accggtgcct ggcctcgctc
    10501 ggttaccgcc ggcgcgggca ggacggtccg ccccatgagc gggagcggat catcctgacc
    10561 tcggcggcgg gtgcgacctt tcgccaggac gcggccgtgc gctactcgct gtgcgagcgg
    10621 gtcacgatca tctgcgggca ctacctcggc gtggacgagc ggatcctgca gttgtacgaa
    10681 gtggacgagg tgtcgatcgg cgactatgtg ctgaccggcg gcgaaccggc cgcggcggtg
    10741 ctggtcgatg cggtcgcccg gctgatcccg aaagtgctgg ggaacttcga gtcggcgctc
    10801 gaagactcct acatgaacca gttgctcggc accccgagct acacccggcc cgccgtgtac
    10861 gaaggcctgg ccgtgccgga gccgctgttg tcgggcaacc acgccgagat ccgggactac
    10921 cgccgccgcc gcgcgcttga gcgggcgctc gaacgccggc ccgacctgct ggcggcggcc
    10981 gaactgaccg atgaggagcg cgcctggatc gcccggcggc ggagagaaca gggcgcgccg
    11041 ccggccgaca gcgccggcgc cgccgatgaa aacgagatgt agcgattaca tattgaacct
    11101 gaaagggata ctacgatgaa acgcattgcc caactcgaaa aaagcatgaa gcgggaggat
    11161 gtccccgcgt tcggctcggg cgatacgctg cgggtccacg tgaaaatcaa agaaggggac
    11221 aaggagcgta tacaggtgtt ccagggaacg gtgatcggcc gccgcggcgg cggcgtcggc
    11281 gaaacgttca ccgtccgcaa aatctccggc ggcgtcggcg tcgaacgcgt ctttcccctc
    11341 cactcgccca acgtggccag aatcgagaaa gtgcgcagcg gcaaggtccg ccgcgccaag
    11401 ctctactacc tgcgcgagct caccgggaag tcggcccgta tccgcgagca actcacggag
    11461 tcgtccgacg acctgacggc ggacgcagcc gagcgggacg ccgccggcga accgaccggg
    11521 cccaagacgc cctccggttc ggcggccgag agcacggacg agccgacggc gtaaggccgc
    11581 agcccgtgtg acgcggcagg cgcccggcct ctgggcgctt cccgccgacc tttgccgttc
    11641 ccggccatgg ccagcgaggt catcaaagtc tgcccctgct gcggtgagcc cttcaccctc
    11701 caggacatcg tcttccgccg cgacatcctg ccgatcggga tgttgctgga ggacgacgat
    11761 ctccggcaca acttctacta tttcaaccac ctcgcgccat cctgcggaac cactttcacc
    11821 atccccgtcg aactcctgcg ggcgcagatc gtcggggaga ttccgtcgga gcggctgagc
    11881 gggacagccc ggtgcggccg gcactgtttg gatctggccg acaccggtcc gtgcgaccgg
    11941 gaatgcgcct acgcacccta ccggcggctg ctgcagtatc tgaaagacgt gcggcgggcc
    12001 gaccgcgcgt ccacaccgcg aatttagacg gccctcgccg agctgtgccg cggggtcatt
    12061 gccgccgcgg caatctcccg atcacgcctc cacgacagtg aacgtggtgt tgccgtaggc
    12121 gcggcgctgc tgaatcgaaa aatcaagaaa gtcgatgcgg ccggtctcgg aactgtcgtg
    12181 ctggcagatg aggaggccgc cgggggcgga gagcgggtgt tcgcggagcg cggccagcgt
    12241 gcggtcgatc atcccccggt actgcggggg cgcgagcatg attacgggcc agcggcggga
    12301 gtcgtcggac aggtgcgcga ttgtccggaa gacgtcgtcg cgcaggcagc ggaggcggtc
    12361 ggcgacgccg agcgcagccg cctggcggtt gatggcatcg gctagcagcc gctcctgctc
    12421 cacccccagg gcgaacgccg cgccccgcga aaccgcctcg aacaggtacg agccggtgcc
    12481 gctgaacaaa tcgaggtagg cggcgccctc gaggtggggg gtgaggaagt cgaagatcgc
    12541 cttgcgcacg cgggagaggg gcgggcgggt cgagccgaga tccggggccg tgatgcgccg
    12601 ccccttgaga tcgcccgcca cgatagtgaa ttcgaactgc cgctgcttcc gtcgcctcat
    12661 gctttccact cgcttccccg cccgcaatat accccccggc ggccgctaaa cacacggctt
    12721 ttccgcgccg atagaatggg ggagagccgg atttttgttg ccgggacggt cccggcgcgg
    12781 cctagttgcg atatggcgga cctcatgacg acccacatgc gaaaaacggc ttccctgccg
    12841 atcgacgacg tcgcggggat gctggcgaac ctcggattcc gcaccatgtg cggcgtcgac
    12901 gaggccgggc ggggaccgct cgccggaccg gtggtggcgg ccgccgtcat tgtgccgcac
    12961 gggttcgctc tcgacggact cactgattcc aagctcatga cgcccgcgca gcgcgacgag
    13021 gtgttcgccc ggatcgccga agcgggcttt ccctgcgcgg tcggcatcat cgaccacgag
    13081 accatcgacc gggtcaacat tctgaaagcc tcgcttctgg cgatgcgccg ggcggtgatg
    13141 gaactgcccg cccctcccga gtgcgtgatt gtcgacggca accacacgat tcctaacctg
    13201 gccttccccc agtacgcgct cgtccacggc gaccggctct gcccggcggt ctcggcggcc
    13261 tcggtgatcg cgaaagtgac ccgcgaccgg atcatggatc gcatggaacg cctctacccg
    13321 cagttccggt tctcccagca caaggggtac tgcacgcccg cccacctcga ggaactccgc
    13381 gcccacgggc cgtgcgagat tcaccgcaag tctttcggcc ccgtcgccga actgctccag
    13441 tatgccctcg tctaagcgcc cccgcggcaa ggggaccggc cgggtggccg ccggccgccg
    13501 ctacgaacgg ctggccgccg atttcttccg cgccaacggt ttcgaagtga gagcgcaaaa
    13561 cttccgcgcc ggagcgcgcg agatcgatct catcgtgcaa cgcgaccgcc tcgtcgtgtt
    13621 cgtcgaggtg aaagccgcgc gcgacgagag cttcgggcac ccggccgaac gggtcgacgg
    13681 acgcaagcgg gaacacctcg tggccgccgc ccggcgctac atgggcgaaa acgacctgag
    13741 cggctgcgat ctgcgcttcg actgcattac ctttgtcggg ggacaaatcg agcactatcc
    13801 caacgcgttc ctggtcgagg agtaggcgcc ggccgctact cgcggcgcac ccgcaccccg
    13861 gggggcggcg ccgaaccggc cgaggtttcc gcgggcgccg tccggatccg caccagcgaa
    13921 tccacgaacc ggctgacctg gcggaagtag tccccggagg ccgacggatc ggcgcgcagg
    13981 cggtcgaccc ggacgcggag cgagtcggcg gtgaggttct cggcggccag cagagagtcg
    14041 cgccaggcca ggaagacggc cgggcggtcg cggtactcgg ctgtgccgac ccagatccgg
    14101 gcggtcagca gggcgaggtg tgcgcgctcc gcctgtgcgg ccgcgcggcg gtgctgccgg
    14161 acgtatgcgt acccgccgac tgcaaggagg acgagcgcga gggccaaaag ccgcctgaac
    14221 cagaccacga tgccaccaac tttcggttga cgcgacgcgt aaaactctgt aaactaccgg
    14281 gcttttcacc cgaggtcaag gctgaacaat ggaactgact taccccatcc ggcgccgcaa
    14341 gagcaaggcg gtcaagatcg gcagcgtgat catcgggggc gggttccccg tcgcgatcca
    14401 gtcgatgacc acggccgata cacgcaacgt cgaggccacg gtcgcgcaga cgcttgacct
    14461 gttcgcggcc ggctgcgaca tcgtccgcct ctccgtgctc aaccatgagg ccgccgactg
    14521 cctgcccgag atccgccgcc gcgtgcccga ccgccccctc gtcgccgaca tccacttcca
    14581 gtacaaactg gcgctcaaag cgatcgcggc cgggttcgat aagatccgca tcaacccggg
    14641 caacatcggg gcggagtgga aagtccgcga ggtgacccgg gcggcccgcg acgccggcgt
    14701 gccgatccgc atcggggtca attccggatc gctgcccaag gatctgctcg agacctacgg
    14761 ccacgacgat ccccgcgcct tcaccgaggc ggccctgcgg gaaatcgcgg tgctccagga
    14821 catggggtgc gccgacatcg tgatttcggt caaatcgacc aacgccgaaa cggccttctg
    14881 ggcctaccac atgctcgcgg cccagaccga gtacccgctc cacgtcggga tcactgaggc
    14941 ggggacgctc gagaccggga gcctcaagtc ggcggtcgga atcggggcga tcctcatgac
    15001 cgggatcggc gacacgatcc gggtgtcgct ctcggccgat cccatccacg aaccgcgggt
    15061 ggcccggcag attctcaccg cctgcaacct gaaaaacgag ggggtgcagg tgatcgcctg
    15121 cccgacctgc ggccgctgcc agatcgacat gatccccctg gcggagtcgg tcgaacggaa
    15181 gacgcgccac atcaaaacgc cgctcaaagt ggcggtcatg ggctgcgccg tgaacgggcc
    15241 gggggaagcc gcggccgccg atgtcggaat cgcgggcggc gacgggatgg ggatgatcat
    15301 ccgcaaaggg caaatcatca agcgcttccc cgaggccgaa ctggagcagc ggctcctgga
    15361 cgagatcgaa gagctgaccg gagaaaagat cccgcgctag gcggcgcccc cgctccggac
    15421 gccgcctcag tcctcgtaca tgtcgaagaa aaccctcagg acctcctgcc agcgtcgcag
    15481 acggtcccgg aactccgcga attcggaatc cgacgccagc caggccttct tcacatcgta
    15541 gccttcggct ctcccttcca gctccagcag gacgttcacc aactggctgt cgggaccgag
    15601 ccggtaccgg cccccgagca tgacttcctt ccagagattg aggtcgaggg gtttggagta
    15661 atttcctttg ccgatgggga gggcgaattc ggcggtctgg gccaggtcga ggtaggccgg
    15721 cagggcggcg gggtcgagca gcagcccgga tgccctgaac ccataggagc ccatgccatc
    15781 ctgcacgtat tgagggggcc agacaaatcg caggaagatg cgatagggaa gcgccggaca
    15841 gctgtagggc gtctccccgc aataggcatc gaggaagttc ttgatgtgag agtcacgcag
    15901 cgccgggacc gcgggaatca aggcggagtc cgagggcagc cggctcagga tcaaccgcag
    15961 tgcatctttg acgccgggta cgatttcgac atcttccgtc tgccatcgtg attcaccggt
    16021 ccacacgggt tgctgacggg gaggcgcgag ggccagctcg atcgtgtgta cgaagccggt
    16081 cgtggtttca atcgcgacgg cggcgctgga gctggtgcgc gtcaacctct gcaccacggc
    16141 ggaggcgacc gcgacgccca ggccgacatc gctcactgaa gcagaacgga agcgcatgcc
    16201 gactgatgat gccgtttccg ccgtcacgga tgcggaagct gcagccgcga attcctccac
    16261 ccgctcggtc cggtacctga gcgtgagcat aagatccgga ttcgactcca cgatctgata
    16321 cccccgcctg gcgagaagtt ctcctgcgat ggaggcgagt tgatcctgga gcaattcctc
    16381 gtggcccggc atcggatcgg tggtcccctc cacccggatc tgaatcgact gcccgatcgg
    16441 gaccgcctgc ccctcgccga gcacccgcgt ccacggccgc catacctcat agcgcgggac
    16501 ggtggcgccg cagccgagga tcaccagcgg cagcagcccg aagactacaa agtgtctcac
    16561 ctgttctcct cccggtgacc gttgtgttgg cgatcagggt tcgcacccga cccgcatgcg
    16621 ccgacggctg cgataccccc gtgttctaat tgtgcctttc gctcccgtat tctatggcgg
    16681 ccgccgcggc ccgtcaactc tagacgttgc ccccggctgt tccggccgcc ccccgccgcg
    16741 ggatttgccg ggaccgcgaa actgcttgcg tcgggaacgg ccgctgtgtt caatggggga
    16801 tccgaaaccg actgtccggc agcttgtttc gaaacggaga cccgccccat gaatcgcatc
    16861 ttgatcgccc tgctgttgtg cctcgccgcc acgccgccct caccgaccgc gaacgtcaaa
    16921 ccgcgagtca gcctccagcc ttccctccgg tacgggttcg gggagaccga gtacgtgctc
    16981 gacgcccgat ggtacgacga cgagggggtg ctccagcatc tgaaaagcca gcttgagttc
    17041 ccgctggacg gcctctatgc ggggctggag gggagggtgc gctggaaccc ggcctcgacc
    17101 aaaccctggg actcgaaact gagcgtgtcc accaacctca gcgacccggc cggcgacatg
    17161 accgaccgcg actggtcgac cttctgggag agcgggagcg agacgttcta cctcccgtcg
    17221 ttcacggagc tctccagcac cgtgtccagc gccgagtcgc ggtcctgggt ggttgcggcc
    17281 gaggtgggca aggtactcgc ggccgggccc aaggggagca tccggctgtt tgcgggctac
    17341 cgctaccagc atatcgcgca gaaggcgatc gggatctcgg ggtggcagat ggtgaatgtc
    17401 gggggggaac tcgtcaaggg ctactttgac ggctcccgcg acgggacgac ctttatcgat
    17461 tacgaggtca cattccacag cccgcaggcg ggcctcgggc tttccctcgc cgtcaccgac
    17521 cgtgcggcgg tggccgcccg ggcggcctac gcgccggtgt ttgcctccga ccgcgacgac
    17581 cacgtggtgc ggaattttct ctcggaggcc tcgatcaacg gccacggcgt catcggcgaa
    17641 gcggaggtcg agtgggagtt gggccggggg gatagctggg ggttgtttct ctcggccggc
    17701 ggcgaattcc tctatttctc cgcctccggc accggcacgc agttttacta cggcgacgac
    17761 cccggcacgc gcaacgaaga tgagaccggg caatacgcgg ccggcgtgcc gcacgagatc
    17821 cacaccaccc aggggcgcgt cgggctgggc ctggcgctgc gcttctagtc ggccggcttg
//
LOCUS       DAOPOE010000068        17877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3769_length_17877_cov_5.684379, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000068 DAOPOE010000000
VERSION     DAOPOE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17877
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_17877_cov_5.684379"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKM94_09635"
     CDS             <1..609
                     /locus_tag="PKM94_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66939.1"
                     /translation="LNYGDFVETDPTGAVLGTFSGGDLLFAVSGAVQPRYGLALGATV
                     KFIYESLHNYTATGAAVDLGGRYSTNRDRLIVGLAVQHLGVQFSGLGTEKDRLPLTVR
                     GGAALRPRGLPLTLAGDLIVPVDNDPSIALGLEYVELKPLYLRIGWNSFGSNYRTGDS
                     DDSWAGLGLGVGFDIKTYHISYAFAPGAELGDSHRFTFSGGI"
     gene            612..1073
                     /locus_tag="PKM94_09640"
     CDS             612..1073
                     /locus_tag="PKM94_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66940.1"
                     /translation="MRVHLLLSAALLSLAAALVGCGSGKPSDPKTAVIAMFGAMEKND
                     KATLAHLLDLGELMKNLNEDYALQTDEPRHFTSPEQILEDLTDGGKTKERWFAYQRIV
                     NEVEMTADDLATVEVTFVDKEASKGYRTKFGLHVVNDRWKIYTFNAFPTGS"
     gene            1736..2254
                     /locus_tag="PKM94_09645"
     CDS             1736..2254
                     /locus_tag="PKM94_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66941.1"
                     /translation="MKKNIGLLVILLVAVVGVMMGTACRDDYYLPAPPSIEGCYTGTY
                     TYVSQSGSEVVVSKEQVITASFTETKFFLDADTLAEGYNKIACFCVGRFFYSISDRVQ
                     IEPIDDRPLIGPGCDSCDPDESPTGTFQLYRPGNDVKLECIETVTSDGGSQLKVTKTL
                     MLTRVACPSTGA"
     gene            2760..4298
                     /locus_tag="PKM94_09650"
     CDS             2760..4298
                     /locus_tag="PKM94_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOD66942.1"
                     /translation="MLAKIFSASTFGVDAYTVEVEADIQQQLPAFVTVGLPDGAVRES
                     KERVTSAIKNSDFVFPAKRVTINLAPADIKKEGSAFDLPIAVGIFAATGQILRDRFDE
                     YVMLGELSLDGALRAVPGVLPMAMNFHTGNGIKGIIVPKDNAREAAMADSVPVYPVDS
                     LKDVIAFLEDESSIAPFKVDIESVFNEAYRYAIDFADVKGQEAAKRALEVAAAGGHNI
                     IMIGPPGSGKTMLARRVPTILPDLTIEEALETTKIHSVAGRLPTGAALIATRPFRSPH
                     HSISYAGLVGGGAVPKPGEVSLAHHGVLFLDELPEFKKDILEMLRQPMEDNEVTISRA
                     STTITYPAGFMLVAAMNPCPCGYFGDPSHECNCSSSEIQRYMARISGPLMDRIDIHIT
                     VPSVNFKDLSSETCGEKSTVIRARVNRARQRQIARFEEEKRIFCNAHMESKDIRKYCL
                     IDDKSQALLATAITRQGLSARAYDRILKVARTIADLGDSDHIDLGHIAEAIHYRTLDR
                     QLWM"
     gene            4471..7158
                     /locus_tag="PKM94_09655"
     CDS             4471..7158
                     /locus_tag="PKM94_09655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD66943.1"
                     /translation="MDRHKLDRLFDPRRIALIGVTINPNSVGGKVLANLVAGGYRGVV
                     YPVNPDSEAVMGIPCYPSVAALPRTPDLAVICAPAEKVPAAVLECGRAGILGIIILSA
                     GFKETGPEGAALEQEIRAARRQFPGMRIIGPNCLGLIVPSRNLNISFAGGMPRAGNVA
                     FLSQSGALCTSVLDWAMEGRIGFSHFVSIGNTIDVDFGDLIDYFGADDSTRSIILYIE
                     SIARAPRFMTAARAFARTKPIIAFKAGRFPESAQVAASHTGALASEDAVYDAAFQRIG
                     LARVYHLANIFDCVQLIGRNRIPTGPNLGIVTNAGGPGVIAADALIAAKGSLARLSDD
                     TLAQLNAALPSSWSHGNPVDILGDATSKRLEKAAQIVLADPAVEALLVILTPQAMTNV
                     TAAARVVGKLAESTAKPVLAAWLGGASMREGMQILTESGVATYRTPEQAVGAFMTLVD
                     YGRNLQTLYETPKDLPVEFELDRARLRADFVARHCGPHPILSEAVSKDLLEGYGIPTA
                     RPRPAADADEAVRIAGELGYPVVIKILSPDITHKTDVGGVALGLEDDAMVRSAFAHMM
                     LAVGEKAPRARIEGVTVQPMIDPRDSLEMILGIKKDATFGTVMMAGMGGVAAELLGDR
                     SLGFPPLNERLARRMLESLKIWPLLQGYRGRPRANVDRLLEVLIRLSYLAADFPEIAE
                     LDINPLLVSPRDVVALDARIVIDPALVGRSSEPYSHLALRPYPEKYVARPTLRDGTTV
                     TLRPIKPEDEPLWLAFLASCSKESIYSRFRYFFHWNSHDVATRFCYIDYNREIAIVAE
                     IVEGGERRIIGIGRLIADPDHEEVEYAVLIADAWQKKELGSLLTDYCMEVASRWKLKR
                     IVAQTTTDNRPMISVFEKRGFAIAFDETGTGVRVVKELA"
     gene            complement(7259..8914)
                     /locus_tag="PKM94_09660"
     CDS             complement(7259..8914)
                     /locus_tag="PKM94_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOD66944.1"
                     /translation="MNTRRYRTAVTALSLALLLVCAGAAAQEPAGRTAEMRNKVDGLL
                     WIGDSILIAVDYGGFVNKTAKMQAFRVTDMAELWYTEVPVGNRLRFEGALWVNPAAGV
                     MYLGHGPFSALDLRDGRVLWSIPYDDIGFVHRVEMGTERLLVLGSKTKSGFRIGLPTS
                     PENAERAAQRAGTGGEHLDDPILFAVNRQSGEIAWQYPFEPGKMHKKKKTDFWSATKI
                     TYEVREPTQLYVIAADGSAGELTMAEGNVIVQAKHVASLDLATGQERWKIKEDFNGAP
                     VFAEDRILVIKEDRLVALNPADGSELWRSKDKVKSAWPSPDPFLVLEGKIVVNSGGKL
                     RVLDPATGAETLKSQKDIGDYTIFSLLLDESTPLCVFPGKYDLSKDGYTENYRLCCLS
                     LADGSLRWQFEKGKEFQSCDLGPGGCIQVIDKERLWRLDPATGKILCECKLGDAVVLA
                     DVPAPTLLGNDGLRCFTPDGKKEAWRYKVKLSDEGVGDAFYTASTLVMPTKKAGIQAI
                     DPNSGTLKWSIEAEKSGLFAASESLTHIALATDKELRVVELKM"
     gene            complement(9003..9671)
                     /locus_tag="PKM94_09665"
     CDS             complement(9003..9671)
                     /locus_tag="PKM94_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66945.1"
                     /translation="MKKTLVIALAAAALIASTAAARTVQDRNWHMCFSMSGSEKLTNL
                     DQLTPAETPAEAAEVSAAVGVNLTGSLAKAISPTFAIGIRGGFFAEGDGGYEGASGGT
                     GTEVLGILEGRCTLNSWLGLKGTAGVGRVSISKDIAKMNVDPDNMLGVDFHIGKISES
                     GLEVLLEPGLEVRLSSVVTLEVLGAYRIRPSKEVEAANLGYDKKITVLDSGFSMGGGI
                     GFHF"
     gene            complement(10028..11434)
                     /locus_tag="PKM94_09670"
     CDS             complement(10028..11434)
                     /locus_tag="PKM94_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD66946.1"
                     /translation="MGEPLNGAAGAVTADVLVADDESAFRSAFGQMLKNRGYRVAMAT
                     DPEEAIATVRRRRFDLVTLDLEWQFSDQTGIDILGRIRKIDPHLPVIIITGHATLETA
                     KQATRLGAYDYLEKMTDREKTLLVVRNAVEAGRLKRAVQQAIDERSKNCEIVGLSRGI
                     QEVREAIAQFGPRDVTVLIEGETGCGKELVARQLHLQSPRRSEPMVTVGGGELSRDLG
                     QDVLYGHTPEAFTGSSRDRGGFVQAADRSTLFLDDISDLPPAMQPLFLRFLETGDYMK
                     LGTDDRQSADVRVIAATNQNLAGLVEQGKFRADLYYRLKVATIAIPPLRERREDIPLL
                     VNHFAAQQSTRLYGRDVSFHPDCHDLFLGREWPGNVRELRNAVIAILCRAANKEVVEP
                     ADVQRVLAEEAGGGGAPLMTLRDMESSFRRQCVVRALVAAGGNVTRAAETLGIDRSHL
                     YRLIAEFDLGQYARQLNV"
     gene            complement(11443..14040)
                     /locus_tag="PKM94_09675"
     CDS             complement(11443..14040)
                     /locus_tag="PKM94_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD66947.1"
                     /translation="MSISHRYRLGLVLCALVTTAMAPSALAAEDKGKEAVCRKLAIPD
                     DPRVLPYRLEPIESQNNPSLTSGEVFLVDVDGDGSAEVGRVWWEVVDSSRFLSTISVM
                     PHSGAEGKLVQYNGPLLSCRFWRNVVFDIWGDSSSEIVLAKHRGDTLFLEIVGFRLDD
                     GVLANDTSAIITAAVARGFPPGEFWHDIIVEPLLATDLNGDGVRELLFTRSAKPDSAI
                     ARGIVAWDFAAGKELWFLPLADMVAGRYRAAYPTAAGETLFVVSTNATANRYECRGMD
                     SHHAYALAFDARGRERWRRVIGGSGFYSVVIPLDVNGDGSQEAVAFCEPAFDSVQTDT
                     ILRALDPATGAVVAGFALGDLPLPRQNPNVLPTGWEEAPIAVSYQADNGRVTVLLNRR
                     LELHRCVRGPALAFMSLGGLLPGEPLLLAAGTPKDKIAILDRNLEPLALTSQGGTLSS
                     GRVDESSRLLLSRGPSDFELVEFQRQSMIVRLSAKYRTHLEIVLAMFLLAVVLKAATS
                     ALNWRRAAMGLPTLDHIKALVLLLDAKGRIVFMNRDPLSRELVGELSGRRPHFLQTRL
                     GAQGELRAVLADSLSDPMQVHQRRIEQTRGADTRQLEVTVYPHVGRHNEYSGKIVVVE
                     DLADRPMWNWRLVLGDAAQRWVHRLKHHMGTARIVLGNLVEDPQIARRLQADPEFRRQ
                     WETADKEILDGGEAATRILKFLRNSKPTLTECDLNDLVRTVASARAGGLPDTVGVSLM
                     LHEGLPPLEADTDQVREVIDNLLSNAIMAVRGEGMITITTRPATGLQSSLEDGVVEVS
                     VADTGAGIAADDLPRIFEPGFSKSPGGTGIGLTLVKDIVENHGGTIAAESELHRGTRL
                     TVRLPLKRR"
     gene            14284..16884
                     /locus_tag="PKM94_09680"
     CDS             14284..16884
                     /locus_tag="PKM94_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66948.1"
                     /translation="MHLAFRSAGAAVLSALLVVLVAASAAAQREPADKDDFADLAAAA
                     PGPKPSIAAHNVGQLTVGVTNFGMIGIGRGKNQRIDCFTGRPVPKGEYPKGSRSVYLY
                     EGGLWVGGVVGGDSLVSTGAEFNNYNFEFWPTQPITTYSNVDPDDPNSALAKSEQDYY
                     ATYVDTMPVSSYDPIANRGHKPLGLRVEQASYAWSYPHTDDFIIITWQIKNISRKAIS
                     DVWVGIYWDADVHLQGIGDMLNRPDYGKPLTDGRDDLAGFMYTVPSADETCPGSDTIG
                     LAWTADNEGDYSSTTGLFAVPNVGAIRFLGPELTDRRYRVNYNWWVYNYSSGYDYGPQ
                     KRDRYRDLGNGPGTPYGDVNRYFMMSNGERDFDMVYTYGIGQNDPVWVPPSRYTSWAM
                     SRGGDVQQVLSVGPRYLAPGSEITVAMAFVCGSGLHYNPVNFRRKMLQAFEPDEYYAG
                     LDFSNLLANAQTAGRVYDIPGFDSNGDGYLGKMRECVVDSAFVDGRWVATALDTSWYE
                     GDGIPDLKAAGPPPAPEFSLHPIRNGVKVLFYGLQSETTRDIFSNQFDFEGYRVYLAR
                     DNRESSFSLVGQYDREDYDKWVLVQKPGRDAQYYRQGDPFTRGFLRCAYGQGPNPCAD
                     SAFDPLLYTISRPYVHPLYPDSVFYFTAHDYNQYAFGAGTSIRKVYPAQEPPVSLQAP
                     APEDTTEEGLFKYYLYEMDIPDLLPSVQYYVVVTAFDFGSPETGLAPQESARTVYAKP
                     AYPNNAWDEKPDSLTNVYVYPNPYRHDAYYRVSGFEGRGEEDRSRDRVRKITFANLPH
                     QCTIRIFTLDGDLVKELNHDIPSSDPNSSYHEWDMVSRNFQMIVSGLYYWVVETPDGR
                     HQVGKLVILL"
     gene            complement(16958..>17877)
                     /locus_tag="PKM94_09685"
     CDS             complement(16958..>17877)
                     /locus_tag="PKM94_09685"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOD66949.1"
                     /translation="IGATTENPSFEVNSALLSRMRVYALDRLSPEGIRGVARRALEDA
                     GRGLGEMRLTVDEEALDFLAAAADGDARRGLSLLEAAATYVGQGGAITAAVLRDIHQK
                     GALVYDKAGEEHYNLISALHKSIRGGDPDASLYWLARMLESGEDPLYIVRRLIRFATE
                     DIGLADPYALTLALNARDTFHFLGSPEGELGIAMAVIYMACAPKSNAAYVAFDRAKAD
                     AAEKGSLPVPLHIRNAPTRLMKDLGYGKGYQYAHDFTDGLTDQEHFPDELAGTRYYEP
                     KGAGREAKMREYLDKYRAFRARGDKPSKS"
BASE COUNT         3073 a         6144 c         5415 g         3245 t
ORIGIN      
        1 ctcaactacg gcgacttcgt cgaaaccgac ccgaccggcg ccgtcctcgg caccttcagc
       61 ggcggcgacc ttctgttcgc cgtgagcggc gcggtgcagc cgcggtacgg tctcgcgctc
      121 ggcgccaccg ttaagttcat ctacgagagt ctgcacaatt acacggccac cggcgcggcc
      181 gtcgacctgg gcggccgcta cagcaccaac cgggaccggc tgattgtcgg cctggccgtc
      241 cagcacctcg gggtccagtt ctccggcctg gggaccgaga aggaccggct gccgctgacg
      301 gtccgcggcg gtgcggccct ccgcccccgc ggccttcccc ttacgctcgc gggcgacctc
      361 attgtccccg tcgacaacga tccctcgatc gccctcggtc tggaatacgt ggagcttaag
      421 ccgctctacc tgcggatcgg ctggaacagt ttcggttcga actaccggac gggcgactct
      481 gatgattcct gggccggact cggcctcggc gtcggtttcg acatcaaaac ctatcacata
      541 tcctacgcct tcgctcccgg ggcggagctg ggcgacagtc accgcttcac cttttccggg
      601 gggatttgac catgagagtg cacctgcttc tctcggccgc cctgctgagc ctcgccgccg
      661 ccctggtcgg ctgcggctcg ggcaaaccgt ccgaccccaa aaccgccgtc atcgccatgt
      721 tcggagccat ggagaaaaac gacaaggcga ccctcgccca cctgctcgac ctgggcgagc
      781 tgatgaagaa tctcaacgaa gattacgccc tgcagaccga tgagccgcgt cacttcacca
      841 gcccggagca aatcctcgag gacctcaccg acggcggcaa gaccaaggaa cgctggttcg
      901 cataccagcg gattgtcaac gaggtggaga tgaccgcgga cgatctcgct accgtcgagg
      961 tcacgtttgt cgacaaggag gcttcgaagg gataccgaac caagttcggc cttcacgtgg
     1021 tcaacgatcg ctggaagata tatacgttca acgccttccc gaccggatct tagttcgccg
     1081 ggaaagggcg cgccacgtgc gtgaaagaca cggcggcacg agtcctcgga ctcgtgcctt
     1141 ctttatggcc ccgggggatc gaagtcaggt cgtgcgatgg cgaggcgggg ctgcaccgtt
     1201 cccttcgcct ccggcgaccg ggccgactcc ggccaaaaaa agaagcccac catgcgccat
     1261 gctcagggac agagggggaa atccccggac aacggtcatc atggcaagct tctaaatctt
     1321 ccacgtaccc cgctcgtcaa aattataggc tacccctcct tgtttgtcaa acacttatcc
     1381 acacgaattc aaagggagag cgtgtgaaaa ttgagggttt ttccgcttct cctccatgga
     1441 gccgtctgta ataaactgta ataaaataac ttaatattta ctgcgtgaga cctcgaccgg
     1501 atttggccgg tccatcattc acgaccgggt tttgggggca cggccaccca ccgcgccaat
     1561 ttctgcgcct ccgcggactc cggccggcgg ttgacgacac gtgcgattgc gctgaacgca
     1621 gcgaatgtgg cggccgtcta aaaaatggag agattgcacg attggggttg tgccgtttcg
     1681 gccggccgcg tatattgacc aaccgttacg gtgttacgga ggagaaaagg acgcaatgaa
     1741 gaagaacatt ggattactgg tcatcttgct ggttgccgtg gtcggtgtca tgatgggtac
     1801 ggcctgccgc gacgattact acctaccagc tccgccaagt atcgaagggt gctataccgg
     1861 cacgtacacg tacgtttccc aaagcggcag cgaggtggtc gtatccaagg agcaggtgat
     1921 cacggcttcc ttcactgaaa ccaagttctt tttggatgcc gacacattgg cggaaggcta
     1981 caacaagatc gcctgtttct gcgtgggtcg ctttttctat tccatctcgg accgcgtgca
     2041 aattgagccg atcgatgatc ggccgctcat cggaccgggc tgcgactcgt gtgatccgga
     2101 tgaatcgccg acaggcacgt ttcagctcta tcggccgggc aatgacgtga aattggagtg
     2161 cattgagacg gtgacgagtg acggcggctc gcagttgaag gtcacgaaga ctctgatgtt
     2221 gacgcgggtg gcatgtccga gtaccggagc gtagcagcat tcgtacagat ggcggtcctt
     2281 ttcgagtttg acggcaggcg gtgagcctgc cgtttttttt gacggcgtca cgtctcacgt
     2341 caagcggcgg gccgccacaa gcgcggccgg ggggccggac tgggtgacgc cccgcctgga
     2401 tgttgttgct gtacttgcgg taaacatcac atcaggcacg cgccccggca gacttttacg
     2461 agtggctggg ccgcacccgc cttcccggcc gcgggtgagc gggcgctacc cggttcgcac
     2521 atggcttcaa tgacagccgg ggcccactcc ggtccacccg accagagccg aaaaaagtga
     2581 tttctcttcg actgctcctg ctgcataatg tcactataat gatgtaactt gtcccaggtt
     2641 aaaccagcgt tgtcctggtc cggcgcgccc ggtacgcaca ctcgatcctg ttgggcgcgc
     2701 ccttgagagc ccagggacgc cacgaagcgg cgccccggcg ccgggggagg gattcagaag
     2761 tgcttgccaa gatcttctcg gcatcaacct tcggggtcga cgcctatacg gtcgaggtcg
     2821 aggcggacat tcagcagcaa ttgcccgctt ttgtcaccgt cggtctgccc gatggcgcgg
     2881 tgcgcgagtc caaagaacgc gtcacctcag ccatcaagaa ctccgatttc gtgtttcccg
     2941 ccaagcgcgt cacgatcaat ctcgccccgg ccgacatcaa gaaagaaggc tctgctttcg
     3001 acctgccgat cgcggtcggc attttcgcgg ccaccggcca gatactccgc gaccggttcg
     3061 acgaatacgt catgctcggc gagctctcgc tcgatggtgc gctgcgcgct gttcccggcg
     3121 tgctccccat ggccatgaac ttccacaccg gcaacgggat caaagggatc attgtcccca
     3181 aggacaacgc ccgcgaggcg gccatggccg acagcgtccc cgtctacccg gtcgattcgc
     3241 tcaaagacgt catcgccttc ctcgaggacg agtcctcgat cgccccgttc aaggtcgaca
     3301 tcgagtcggt cttcaacgaa gcctaccggt atgcgatcga ctttgccgat gtcaagggcc
     3361 aggaggcggc caagcgggcc ctggaggtgg ccgcggccgg cggccacaac attatcatga
     3421 tcggccctcc cgggtcgggc aagaccatgc tggcgcgccg cgtaccgacc atcctgcccg
     3481 acctcaccat cgaagaagcc ttggagacca ccaagattca ttcggtcgcc ggccgcctgc
     3541 ccaccggcgc cgccctcatc gccacccgcc cgtttcgctc gccgcaccat tcgatttcct
     3601 acgccggtct cgtgggcggg ggagccgtcc ccaaaccggg cgaagtctct ctcgctcacc
     3661 acggcgtgct gtttctcgat gaactccccg aattcaagaa agacatcctc gaaatgctgc
     3721 gccagccgat ggaggacaac gaggtgacta tctcgcgtgc gagcaccacg atcacctacc
     3781 cggccgggtt catgctcgtg gccgccatga acccctgccc ctgcggctat ttcggcgacc
     3841 cgagccacga gtgcaactgc tcctcgagcg agattcagcg ctacatggcg cgcatctcgg
     3901 gaccgctcat ggaccgcatc gacatccaca tcaccgtccc gtcggtgaat ttcaaagacc
     3961 tctcctcgga gacctgcggc gagaaatcga ctgtcatccg ggcccgggtc aaccgcgccc
     4021 gccagcgcca gattgcccgg ttcgaggagg agaaacggat cttctgcaac gcccacatgg
     4081 agtcgaagga tatccggaag tactgcctga tcgacgacaa gtcgcaggcg ctcttggcga
     4141 ccgcgatcac gcgccagggg ctgtcggccc gggcctatga ccgcattctg aaagtcgcgc
     4201 ggacgatcgc cgatcttggc gacagcgacc acatcgacct tggccacatc gcggaggcga
     4261 ttcactaccg gaccctcgac cggcagttgt ggatgtgagg ggcaagtgaa ggcgcacgag
     4321 cggggcgcgg cgtccgccgg ccgcaccctg tcagggggca gtcgcggcgc tgtcgcatca
     4381 gatgcggcgc ccggcccccc tcccccaaaa attcccccac gccgctccga aatgtcctac
     4441 attatccccg ttgccgtgca ggagatgaca atggacagac ataaactcga ccgcctcttt
     4501 gacccccgcc gcatcgccct catcggcgtc acgatcaacc ccaattccgt gggcggcaaa
     4561 gtcctcgcca acctcgtcgc cggcggttac cgcggcgtgg tctatcccgt gaacccggac
     4621 tccgaggcgg tcatgggcat cccgtgctac ccctcggtcg cggcgctccc gcgcacaccc
     4681 gacctcgcgg tcatttgcgc gccggcggag aaagtccccg cggccgtgct cgaatgcggc
     4741 cgggccggca tcctcggcat catcatcctc tccgccggtt tcaaggagac cggccccgag
     4801 ggggcggcgc tggagcagga gatccgggcc gcccggcggc agtttcccgg catgcgcatc
     4861 atcggtccca actgcctcgg cctcattgtc ccctcgcgca acctgaacat cagtttcgcc
     4921 ggcgggatgc cccgggccgg caacgtggct ttcctctcgc agtcgggcgc gctgtgcacc
     4981 tcggtgctcg actgggccat ggaggggcgg atcggtttct cccacttcgt gtcgatcggc
     5041 aacacgatcg acgttgattt cggcgacctc attgattatt tcggcgccga cgattcgacc
     5101 cgctcgatca ttttgtacat tgagtcgatt gcgcgggccc cgcgcttcat gaccgccgcg
     5161 cgcgcgttcg cgcgcacgaa accgatcatt gccttcaaag ccggccgttt tccggaatcg
     5221 gcgcaggtcg cggcctcgca caccggggcg ctcgcctcgg aggacgccgt gtacgacgcc
     5281 gcctttcagc gcatcggtct cgcccgcgtc taccacctgg ccaacatttt cgactgcgtg
     5341 cagctcatcg ggcgcaaccg cattccgacc ggccccaacc tgggcatcgt caccaacgcc
     5401 ggcggcccgg gcgtgatcgc cgccgacgcc ctcatcgcgg ccaaggggtc gctcgcccgg
     5461 ctctccgacg acaccctggc gcagctcaac gccgcgctcc cctcctcgtg gtcgcacggc
     5521 aacccggtcg acatcctcgg cgacgcgacc tcgaaacggc tcgagaaggc cgcccagatt
     5581 gtgctcgccg atccggcggt ggaggcgctg ctggtcattc tcacgccgca ggcgatgacc
     5641 aacgtcacgg ccgccgcccg ggtggtcggc aaactcgcgg agagcacggc caagccggtt
     5701 ctcgccgcct ggctcggcgg cgcctccatg cgcgagggga tgcagattct caccgagagc
     5761 ggcgtcgcca cctaccgcac gcccgagcag gcggtggggg ccttcatgac cctcgtcgac
     5821 tacggccgca acctgcagac gctgtacgag accccgaaag atctcccggt cgaattcgag
     5881 ctcgaccggg cgcggctgcg cgccgatttc gtcgcccgcc actgcggccc tcatccgatc
     5941 ctgtccgagg ccgtctcgaa agacctcctc gagggttacg gcattcccac ggcgcgtccg
     6001 cggccggccg ccgacgccga cgaggcggtg cggatcgccg gcgaactcgg ctaccccgtc
     6061 gtcatcaaga tactctcccc cgacatcacg cacaaaaccg acgtcggcgg ggtggcgctc
     6121 gggctcgagg acgacgccat ggtgcgcagc gcgttcgccc acatgatgct cgcggtgggg
     6181 gagaaggcgc cgcgggcgcg catcgagggg gtcacagtcc agccgatgat cgacccgcgc
     6241 gacagtctcg agatgattct cgggatcaag aaggatgcga cgttcgggac ggtgatgatg
     6301 gcggggatgg gcggggtggc ggccgagctg ctgggtgacc ggagtcttgg ctttccgccg
     6361 ctcaacgagc ggctcgcccg ccgcatgctc gaatccctca agatctggcc gctcctccag
     6421 ggctaccgcg gccgccccag ggccaatgtt gaccgccttc tcgaagtcct catccgcctg
     6481 tcgtacctcg cggccgattt ccccgagatc gccgaactcg acatcaaccc gctgctggtc
     6541 agcccgcgcg acgtggtcgc gctcgacgcc cgcatcgtca tcgaccccgc gctcgtcggc
     6601 cggtcctccg agccctattc ccacctcgcc ctgcgcccct acccggagaa gtacgtcgcg
     6661 cgccccacgc tccgcgacgg cacgacggtt actctccgcc cgatcaagcc cgaggacgaa
     6721 ccgctctggc tggctttcct cgcgagctgt tcgaaagagt cgatctactc ccgcttccgc
     6781 tacttcttcc actggaattc gcacgacgtc gccacccgct tctgctacat cgactacaac
     6841 cgcgagatcg ccattgtcgc cgaaatcgtc gagggcgggg agcgccggat catcggcatc
     6901 ggccgtctca tcgccgaccc cgatcacgag gaggtggagt acgccgtgct tatcgccgac
     6961 gcctggcaga agaaggagct cggcagcctc ctgacggact actgcatgga ggttgcctcg
     7021 cgctggaagc tgaagcggat tgtcgcccag accacgaccg acaaccggcc gatgatttcg
     7081 gtgttcgaga agcgcggttt cgccatcgct ttcgacgaaa ccggcaccgg cgtgcgagtg
     7141 gtgaaggagc tggcctagcg agcgggagca ggatgcccgg cccgccgcaa tcgccgggcg
     7201 cggcgaagcg cgcggtgcgg ccggcgcggg catcccgcct tgtctctctc gcccctcctc
     7261 acattttgag ctccaccacc ctcagctctt tgtcggtcgc cagcgcgatg tgcgtcagcg
     7321 actccgaggc cgcaaacagc cccgacttct ctgcctcaat gctccacttg agcgtcccgg
     7381 agttcgggtc aatcgcctga atccccgcct tcttggtggg catcacgagc gtcgaggccg
     7441 tgtagaaagc gtcccccacc ccttcgtcgg acagcttcac cttgtatcgc cacgcctcct
     7501 tcttgccgtc gggcgtgaag cacctgaggc cgtcgttgcc aagcagggtc ggcgccggga
     7561 cgtcggccag gacgacggca tcgccgagtt tgcactcgca caggatcttg ccggtcgcgg
     7621 ggtcgagccg ccacagccgc tccttgtcga tcacctggat gcagccgccc ggaccgaggt
     7681 cgcagctctg gaactccttc cccttctcga actgccagcg gagcgatcca tcggcgagcg
     7741 acaggcagca gagacggtag ttctcggtgt acccgtcctt gctcaggtcg tacttgcccg
     7801 gaaacacgca gagcggagtc gactcgtcga gcaaaagcga gaagatggtg tagtcgccga
     7861 tgtctttctg gctcttgagc gtctccgcgc ccgtggccgg atcgagcacc cgcagcttgc
     7921 cgccggaatt cacgacgatt ttcccctcga gaaccaggaa gggatcgggc gatggccagg
     7981 cgctcttcac tttgtccttg ctccgccaca actccgaccc gtcggccggg ttcagcgcca
     8041 ccagacggtc ctccttgatc acgaggatgc ggtcctcggc gaagaccggc gcgccgttga
     8101 agtcctcctt gatcttccac cgctcctgcc cggtggccaa gtcgaggctg gcgacgtgtt
     8161 tggcctggac gatgacgttc ccctccgcca tggtcaattc gcccgcgctt ccgtccgccg
     8221 cgatgacata gagctgggtg ggctcccgca cttcgtaggt gatcttggtg gccgaccaga
     8281 aatcggtctt cttctttttg tgcatcttcc ccggctcgaa gggatactgc caggcgatct
     8341 cgcccgactg ccggttcacg gcgaagagaa tcgggtcatc gaggtgttcg ccgccggtgc
     8401 cggcgcgctg ggccgcgcgc tcggcgttct cgggcgaggt gggcaatccg atgcggaagc
     8461 cggacttggt tttcgacccc agcacgagca ggcgttcggt ccccatctcg acgcggtgga
     8521 caaacccgat atcgtcgtag gggatcgacc agagaacccg tccatcgcgc aggtcgagcg
     8581 ccgagaaggg tccgtggccg agatacatca cacctgccgc gggattcacc cacagcgccc
     8641 cttcgaaccg caggcggttg ccgacaggca cctccgtgta ccacagctcg gccatatcgg
     8701 tcacgcgaaa ggcctgcatc ttcgcggtct tgttgacgaa tccgccgtag tcgacggcga
     8761 tgaggatact gtcgccaatc cacagcaggc cgtccacttt gttgcgcatc tccgccgtcc
     8821 ggccggcggg ttcctgggcg gcggcgccgg cgcacaccag aaggagcgcc agactcagcg
     8881 ccgtcaccgc ggttcgatat cttctcgtgt tcatgattga ctcctcacct gtgcgacgtg
     8941 actctatgcg gcggccgcgg cagggccgcg acgcgaccct gccgccggac cactcctctg
     9001 tctcagaaat ggaaaccaat gccgccgccc attgagaatc ccgagtcgag caccgtgatc
     9061 ttcttgtcgt agccgagatt cgccgcctcg acctctttcg acggccggat gcggtacgcc
     9121 cccagcacct cgagcgtcac cacgctcgac agacggacct ccagacccgg ctccagcagg
     9181 acttccaacc cgctttccga tatttttccg atatgaaagt ccacgcccag catgttgtcc
     9241 gggtcgacat tcattttggc gatgtctttg gagatgctga cccggccgac tcccgccgtc
     9301 cccttcaacc ccagccagct gttgagcgtg caccggccct cgagaatccc cagcacttcg
     9361 gtcccggtgc ctcccgacgc gccttcgtat cccccgtccc cttcggcgaa aaacccgccc
     9421 cggatgccga tcgcgaaagt cggcgaaatg gctttcgcga gcgagccggt cagattgacg
     9481 cccacggcgg cggagacctc ggccgcctcg gcgggcgtct cggccggcgt caactggtcg
     9541 aggttggtga gtttctcgct ccccgacatc gagaaacaca tatgccagtt gcgatcctgc
     9601 accgtgcggg cagccgcggt cgacgcgatg agcgccgcag ccgccagagc gataaccaac
     9661 gtcttcttca tgggacacct ccagacgatt cctttgtgtt atgcgatcag tttttgagtc
     9721 tcggagtttg ggtttcgcat cgttgccgtc tctttagtgg attcgctcat ctttgttcga
     9781 gccttcatgc aatctccgtg ccggagctgc ggcggttccg tatttcgccc cgcggcaacg
     9841 gtttgcctcg gatacaggca gccgcggccg gcctgctgtc gcacgcgtgc gacggcgggc
     9901 gccggcgggt gtcgcagatc tgcgacgccg ggccgcgcgg tcgcgccggt tgcgggaact
     9961 cggtatataa tgagaaaggc ggcagccgat cggccgccgc cggagcgccc cgccgtgggg
    10021 cggtcaccta gacattgagc tggcgggcat actggccgag atcgaattcg gcgatcagcc
    10081 ggtacaggtg cgagcggtcg atcccgagcg tttccgcggc ccgggtgaca ttcccgccgg
    10141 ccgccaccag cgcccgcacc acgcactgac gccggaaact gctctccatg tcgcgcaggg
    10201 tcatgagcgg cgcgcctccc ccgccggctt cttcggcgag cacccgctgg acatcggccg
    10261 gctccacgac ctccttgttg gccgcgcggc acagaatcgc gattacggcg ttgcgcagtt
    10321 cgcggacgtt gcccggccac tcccggccca ggaacaggtc gtggcagtcc gggtggaacg
    10381 atacgtcgcg cccgtacagc cgcgtcgact gctgggctgc gaagtggttc accagcagag
    10441 ggatatcctc gcgccgctcc cgcagcggcg gaatggcgat ggtcgccact ttcagccggt
    10501 agtagagatc agcccgaaac ttcccctgct ccaccaggcc ggccagattc tggttggtgg
    10561 ccgcgatcac ccggacatcg gccgactgac ggtcgtcggt tccgagtttc atgtagtcgc
    10621 cggtctccag aaagcggagg aagagcggct gcatggcggg tgggaggtcg ctgatgtcgt
    10681 cgagaaacag ggtcgagcga tcggccgcct ggacgaaccc gccgcggtcg cggctggaac
    10741 cggtgaaggc ttcgggcgtg tggccgtaga gcacatcctg cccgaggtcc cggctcagtt
    10801 cccctccgcc gaccgtcacc atcggctcgc tgcgccgcgg gctctggagg tggagctggc
    10861 gcgccaccaa ttccttgccg cacccggttt ccccttcgat cagcaccgtg acgtcgcgcg
    10921 ggccgaactg cgcgatcgcc tcgcgcacct cctggatccc ccggctgagg ccgacaatct
    10981 cgcagttttt gctgcgctcg tcgatggcct gctggaccgc ccgtttgagc cggccggcct
    11041 ccaccgcgtt gcgaaccacg aggagggttt tctcccggtc ggtcattttc tccaggtaat
    11101 cgtacgcccc gaggcgcgtg gcctgcttgg ccgtctcgag cgtcgcgtgc ccggtgatga
    11161 tgatgaccgg caggtgggga tcgattttgc ggatccgtcc gagaatgtcg atacccgtct
    11221 gatcgctgaa ttgccactcc aggtcgaggg tgaccaggtc gaaccgccgt cgccggactg
    11281 tcgcgatcgc ctcctccggg tcggtcgcca tcgccacacg gtagccgcgg tttttgagca
    11341 tctggccgaa tgcgctccgg aacgccgatt cgtcgtcggc caccagcaca tcggccgtta
    11401 cggccccggc tgctccattg agcggctcgc ccatgtcggg tctcaccttc ttttgagcgg
    11461 cagacggacc gtaagtcgcg tcccgcggtg gagttcgctt tcggcggcga tcgtcccgcc
    11521 gtggttttcc acaatatctt tcaccagcgt cagtccgatg ccggtgccgc cgggcgattt
    11581 gctgaacccc ggctcgaaaa tccgcggcag atcatccgct gctattccag cacccgtgtc
    11641 ggccaccgac acctcgacga cgccatcctc cagactgctc tgcaaaccgg ttgccggccg
    11701 cgtcgtgatc gtgatcatcc cctccccgcg gacggccatg atcgcattgg acaaaaggtt
    11761 atcgattacc tcgcgaacct ggtcggtgtc cgcctcgagc ggcggaagcc cctcgtgtag
    11821 cattaatgag acgcccactg tgtccggaag gccgcccgcg cgagcgctcg ccaccgtgcg
    11881 gaccaggtcg ttcagatcgc actcggtcag ggtcggcttg ctgttgcgca gaaacttcag
    11941 aatccgggtc gccgcttccc cgccatcgag aatttccttg tccgcggttt cccactggcg
    12001 gcggaattcc ggatcggcct gcagccgccg ggcgatttgc gggtcctcga ccagattgcc
    12061 cagcacaatc cgggcggtcc ccatgtggtg tttgagccgg tgcacccacc gctgcgcagc
    12121 atcccccagc accagccgcc agttccacat cggccggtcc gccagatctt ccaccacgac
    12181 gattttcccg ctgtactcat tgtgccggcc gacgtgcggg tagacggtca cctcgagctg
    12241 gcgggtgtcg gcgccgcggg tctgttcgat gcggcgctgg tgcacctgca tggggtcact
    12301 cagcgagtcg gcgagcaccg ccctcagttc accctgcgca cccagccggg tttgcaggaa
    12361 gtgcggtcgc cgcccgctca attccccgac cagttcccgg ctcagcggat cgcggttcat
    12421 gaagacgatc cgccccttgg cgtccagcaa cagcacgagg gccttgatgt ggtcaagcgt
    12481 cggcagcccc atcgccgccc gacgccagtt gagagcggat gtcgcggcct tcaggaccac
    12541 cgccagcagg aacatggcga ggacaatttc caggtgtgtg cggtatttgg cgctcaagcg
    12601 gactatcatc gactgacgtt gaaactccac caactcgaaa tccgagggcc cccgcgacag
    12661 tagcaaacga ctgctttcgt cgacccggcc cgatgaaagc gtgcctccct gcgatgtcag
    12721 cgcgagcggc tccagattcc gatcgagaat cgcgatctta tccttcgggg tacctgcggc
    12781 cagcagcaac ggttctcccg gcaacaaccc gccaagagac atgaaggcca gggccggtcc
    12841 gcgcacgcac cggtgcaact ccagtcggcg gttcagcagt actgtcactc tgccgttgtc
    12901 ggcctggtag gaaacggcga tcggggcctc ctcccagccg gtcggcagaa cattcggatt
    12961 ctggcgaggg agcggcaagt ctcccagcgc aaagccggcc accacggcgc cggtcgcggg
    13021 atcgagcgct cgcaggatgg tatcggtctg cacgctgtca aaggccggtt cgcagaaagc
    13081 aaccgcctct tgagatccgt cgccgttgac atcgaggggg atcacgaccg agtagaagcc
    13141 gcttcccccg atcacgcgcc gccacctctc gcggcccctg gcatcgaaag ccagcgcgta
    13201 ggcgtggtgg ctgtccatcc cgcggcactc atacctgttg gcggtcgcat tggtggacac
    13261 aacgaagagt gtctcccccg ccgccgtcgg ataagccgcc cggtatcgcc cggccaccat
    13321 gtcggccagc ggcagaaacc acaattcctt tccggccgca aaatcccacg ctacgatacc
    13381 gcgcgcgatc gccgagtccg gtttcgcgct tcgggtaaac agcagctcgc gaacaccgtc
    13441 tccattgaga tctgtcgcca ggagcggctc gacgatgatg tcatgccaga actcgccggg
    13501 aggaaaacct ctcgccacgg cggcggtgat gatcgctgag gtatcgttcg ccagcacgcc
    13561 gtcgtccaat cgaaaaccga caatctccag aaacagggtg tcgccgcggt gcttggcgag
    13621 cacgatttcg ctggaggaat ctccccagat gtcaaacaca acgttccgcc agaaccggca
    13681 cgagagcagc ggaccgttgt actgaaccag cttgccctcc gctcccgagt gcggcatcac
    13741 tgagatcgtg ctcaagaacc gggaagagtc caccacctcc caccagaccc ggccgacctc
    13801 cgcgctcccg tcgccgtcga catcaaccag gaaaacctct ccgctggtga gcgaggggtt
    13861 gttctgagac tcgattggct ccagccggta cggcagcacc cgcgggtcat ccggaatggc
    13921 aagcttccgg caaaccgctt ccttcccctt gtcctccgcc gcaagcgccg acggcgccat
    13981 ggcggtggtc acgagcgcac acaggactaa cccgaggcga tagcgatggg aaatcgacat
    14041 ctcatctcat tcagataaac acccttgcct cggcaatata gccactgcca tccctcttgt
    14101 cgagatcttc ttccgtagcc tggcgggcgg gcggccaggt cgcgggcggt cgcctgctaa
    14161 ttcctgcgct cgtccgactc ggtgaatccg cttgtctggc cggaactttg cccatatctt
    14221 cgcgtaagtg ctttcgatat ttgcgtctct gcgtgactac tttgtgagga cggaatcgtc
    14281 tctatgcact tagcctttcg ttccgccggc gccgccgtgc tctccgcgct gttggtcgtt
    14341 ttggtggccg cctcggccgc cgcccagcgg gagcctgccg acaaggatga tttcgcggac
    14401 ttggcagccg ccgcgcccgg ccccaagccc agcatcgccg cccacaacgt cggccaactc
    14461 accgtgggcg tcaccaattt cggcatgatc ggcatcgggc ggggcaagaa ccagcgcatc
    14521 gactgtttca ccggacggcc agtcccgaag ggggagtacc ccaagggctc gcgctcggtg
    14581 tacctctatg agggagggct ctgggtgggc ggcgtggtgg gcggcgacag cctggtctcc
    14641 accggtgccg agttcaacaa ctacaatttc gagttctggc ccactcagcc catcaccacc
    14701 tactccaacg tcgaccctga tgatcccaac tccgccctgg ccaaatccga gcaggactac
    14761 tacgcgacct atgtcgatac catgccggtg agttcgtacg atccgatcgc caaccgcggg
    14821 cacaagccgc tcgggcttcg ggtcgaacag gcctcctacg cctggtccta cccgcacacc
    14881 gacgatttca tcatcatcac ctggcagatc aagaacattt cgcggaaggc catttcagac
    14941 gtgtgggtgg gcatctactg ggatgccgat gtgcacctgc aggggatcgg cgacatgctc
    15001 aaccgccccg actacgggaa gccgctcacg gacgggcgcg acgacctcgc ggggttcatg
    15061 tacaccgtgc cgtcggccga tgagacctgc cccggctccg ataccatcgg cctcgcctgg
    15121 accgccgaca acgaaggcga ctactccagc acgaccgggc tgttcgcggt tcccaacgtg
    15181 ggggccatcc gctttctcgg ccccgagctg accgatcgcc gctaccgtgt gaactacaac
    15241 tggtgggtct acaactacag ttccggctac gactacggtc cgcagaagcg ggaccgctac
    15301 cgcgacctcg gcaacggacc ggggaccccg tacggcgacg tcaaccgcta cttcatgatg
    15361 agcaatggcg agcgggattt cgacatggtc tacacgtacg gcatcggcca gaacgacccg
    15421 gtgtgggttc ccccgagccg ctacacgtcc tgggcgatgt cccggggcgg cgacgtgcag
    15481 caggtgctct cggtcggccc gcgctacctc gcccccggct ccgaaatcac ggtcgccatg
    15541 gccttcgtct gcggctccgg cctgcactac aacccggtca acttccgccg caagatgctc
    15601 caggcgttcg agccggacga gtactacgcc gggcttgact tctccaatct cctggccaat
    15661 gcccagaccg ccggccgcgt ctatgacatc cccggtttcg acagcaacgg cgacggttac
    15721 ctggggaaga tgcgcgagtg cgtggtcgat tcggcgtttg tcgacggccg ctgggtggcc
    15781 acggcgctcg acacgagctg gtacgaaggg gacgggatcc ccgacttgaa agcggccggc
    15841 ccgccccccg cccccgaatt ctcgctccac cccatccgca acggcgtgaa agttctcttc
    15901 tacggactcc agtcagagac cacccgggac atcttctcca accaattcga cttcgagggc
    15961 taccgcgtct acctcgcccg cgacaatcgg gagagcagct tctcgctcgt cggccagtac
    16021 gaccgcgagg attacgacaa gtgggtgctc gtgcagaaac ccgggcgcga cgcgcagtac
    16081 taccgccagg gggatccgtt cacccgcgga tttctccgct gcgcctacgg ccaggggccc
    16141 aacccgtgcg ccgattccgc ttttgacccc ctcctctaca ccatctcccg cccgtacgtg
    16201 cacccgctct atcccgactc ggtgttctac ttcacggctc acgactacaa ccagtatgcc
    16261 ttcggggccg ggacctcgat ccgcaaggtc tatccggcgc aggagccgcc cgtgtcgctg
    16321 caggcgccgg cgcccgagga cacgaccgag gaaggtctgt tcaagtacta tctgtacgag
    16381 atggatatcc ccgacctgct cccgagcgtg cagtactacg tggtggtgac cgcattcgac
    16441 ttcgggtcgc ccgagaccgg cctggccccg caggagtccg cgcgcacggt ctacgcgaag
    16501 ccggcttatc ccaacaacgc ctgggacgag aagcccgact cgctgaccaa cgtgtacgtc
    16561 taccccaacc cgtaccggca cgacgcctac tatcgcgtct ccggtttcga gggacggggc
    16621 gaggaggacc gctcgcgtga tcgtgttcgc aagattacgt ttgccaatct gcctcaccag
    16681 tgcacgatcc gcattttcac gctcgacggc gacctggtga aagaactcaa ccacgacatt
    16741 ccgtccagcg atccgaactc ctcctaccac gagtgggaca tggtgagccg gaacttccag
    16801 atgatcgtct ccgggttgta ctactgggtg gtggagacgc cggacgggcg gcatcaggtg
    16861 ggcaagctcg tgattctgct gtgaggggat ggcggccgcg gcggcccgag gcggcggccc
    16921 ggccggacgg tccgcgcccg gcgcgcctcc ggaggggcta ggacttcgag ggtttgtcgc
    16981 cgcgggcccg aaacgcccgg tacttgtcca ggtactctcg catcttggcc tcccgtcccg
    17041 cgcctttcgg ctcgtagtag cgtgtccccg ccagttcgtc ggggaaatgc tcctggtcgg
    17101 tcagcccgtc ggtgaagtca tgggcgtact ggtagccctt gccgtagccg aggtctttca
    17161 tcaggcgggt cggggcgttg cggatgtgca gggggacggg cagcgatccc ttttcggccg
    17221 catcggcctt ggcccggtca aacgccacat aggcggcgtt ggatttgggc gcgcaggcca
    17281 tgtagatcac agccatggca attcccagtt ccccctccgg cgaaccgaga aaatggaaag
    17341 tgtcgcgcgc attgagcgcc agcgtcaggg cataggggtc ggccagtccg atatcctcgg
    17401 tcgcgaaccg aatcagccgc cgcacgatgt agagcggatc ctcgcccgac tcgagcatgc
    17461 gcgccagcca gtacagggag gcatccgggt cgcccccccg gatcgacttg tggagcgcgg
    17521 aaatgaggtt gtagtgctcc tcccccgcct tgtcgtacac cagcgctccc ttctggtgga
    17581 tgtcgcggag cacagcggcg gtgatggcgc cgccctggcc gacataggtc gccgccgcct
    17641 cgagcagcga cagcccccgc cgcgcgtcgc cgtcggcggc cgccgcgagg aaatcgagcg
    17701 cctcctcatc gaccgtcagg cgcatctccc ccagcccccg cccggcgtcc tccagcgccc
    17761 ggcgcgccac tccgcggatc ccttccggcg acagccggtc gagcgcgtag acccgcatcc
    17821 gcgacaacag agccgagttg acctcgaacg agggattctc ggtggtcgcc ccgatca
//
LOCUS       DAOPOE010000069        17311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_3933_length_17311_cov_6.707696, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000069 DAOPOE010000000
VERSION     DAOPOE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_17311_cov_6.707696"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /locus_tag="PKM94_09690"
     CDS             complement(<1..2348)
                     /locus_tag="PKM94_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD66950.1"
                     /translation="MRAVFVLVMLTVLCGFVFAEDFRVGQSCVPPSGDEYVPDEIVVK
                     FKDGVGRQAVSAALADLGATTISTNRQIGFHQLRIPAGTTVEEMVAAFGARPDVEYAE
                     PNYIAHAFMTPNDPYYTYQWHMPLIKMPAAWDQTTGSPSVIVAVVDCGVAYETYGSFT
                     QAPDLAGTSFVPGYDFINNDSHPNDDCAHGTHVAGTVAQTTNNNLGVTGVAFNCSIMP
                     VKVLDASGSGSYTAIANGVTFAADNGAHVINMSLGGSYNSSTLQNAVVYAYNKGVTIV
                     CAAGNAGTSTPQYPAAYSQCISVSAVRYNKTYTSYTSYGSTIDICAPGGDVSVDQNGD
                     GYVDGVLQQTHDGTNYGTFGYYFYEGTSMASPHVAGVAALLIAKNGGSMAPADVRAAL
                     QNTAEDLGAAGWDQYYGYGLVDANAALLSISTNNPPVAAFSGTPTSGTAPLTVVFTDA
                     STNNPTSWSWNFGDGGTSTAQNPTHTYASAGTYTVTLTATNAYGSDSETKTGYITVSA
                     PPTYPPVAAFSGTPTSGTAPLTVVFTDASTNTPTSWSWNFGDGGTSTAQNPTHTYTSA
                     GTYTVTLTATNAYGSDSETKTNYITVTTETAQCDDFNDGNYTGWSNKLGTWSAANYYL
                     KGNSTTSNARLTSPFGTFSTATITCKVRMNTGRTQRNTRIIFAYTNSSNYRYVQFDDY
                     YNRVYVGEILSGRQYTRAYASYTFSTGVWYDVTVSVAASGTVSMMVGSTSIGSYTFSA
                     RAGLVGVGYTKSNSDFDDFCVNAAASWAEAPAAPAELVPAGF"
     gene            complement(2649..3152)
                     /locus_tag="PKM94_09695"
     CDS             complement(2649..3152)
                     /locus_tag="PKM94_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOD66951.1"
                     /translation="MENDFTVEVLVEIPKGSRNKYEYDKARGLFRLDRMLFSAVHYPS
                     DYGFILNSLGGDRDPLDALVLTWEPTFPGCLIDARPVGLFRMQDEKGPDEKILCVPIH
                     DPMWNRIRALEEVPPHLVKEIEHFFQVYKELEEKKTAVEGWEDRAAAERVVREAQERW
                     RKAGEHD"
     gene            complement(3295..5109)
                     /locus_tag="PKM94_09700"
     CDS             complement(3295..5109)
                     /locus_tag="PKM94_09700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOD66952.1"
                     /translation="MSHTATHIPAAPTWDLDSIFPGGSASAEYKAFREKVKKDLAGAA
                     TAVDRLPAAITPENRPQWAAAISTLQSILADLELLLAFASCLKAQNVSDTLAHAADAE
                     GEEDVAQWKKLMTRIEAMSLQQSDAAWQLLTAAPELRGTEFFLNEVRETARRKMPVEK
                     EELALDLAVNGYHTWNQLYDKMAGDLREPFEEGGQTKLLSMGQIATKMSSPDREVRRR
                     AFEAMTNGWKRRQDLAAMALNAQAGFRLSLYKYRGWDLMYEPLRNARLQEATLDTMWR
                     VIAEEKKRLRAYVDAKKRLLGIDRFRWYDEFAPAGRSDRQYSFAEAGDFVVAQVRSFS
                     DDMADFYRRAIDSRWVEAEDRAGKAGGAFCTGFGPVRQSRVFMTYGGSFENLLTLAHE
                     LGHAYHQQVITEKPYFAQQYPMPLAETASIFSETLVTDAALGQAADAGERLMLLDQKL
                     QSAYVMFTDLHCRYIFDRAFYAERRNGMVPAERLSEMMVTAQKEAFAGMLDDSGCHPL
                     FWASKLHFYMTSAPFYNFPYVFGFLFAGGVYDRAKREGKKFAKDYRALLADTGSMTCE
                     ELARTHLGVDLADEGFWRSAVDRALAEVEAYVKLAKSM"
     gene            complement(5273..8203)
                     /locus_tag="PKM94_09705"
     CDS             complement(5273..8203)
                     /locus_tag="PKM94_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOD66953.1"
                     /translation="MAGTLSGRRGSRGALALRRLSFIAALILCAAAPAGGKDLYHVTL
                     TGPTDAEALRGLGLDAVARVADGYLVLIDPPQAAALETSALRSRLVRANIARSDLARD
                     LRTDPGDRARYPVVFESGGLRLVDVRGGIVPDENGWLGLAPLPSTSLPIVCRTARPIG
                     RARPERALDLDSLISLINEDSCRSYVERLQAFGTRKVGTSQNAASRDWLVDKFRSFGY
                     DSVITDSFAAYENWGDGPAVAAFNVIAVKPGTLYPDFHILIGAHRDSSPRQSPGADDD
                     ASGIAGVLEIARVAAALETPVSLVFAAFDAEECGLWGSDYYAAEAARREDSIALVLNM
                     DMIGYHENDSAVWVYHTVPGSYGALWGALADSLAGVDLSAHLAAGAAWDAEPFEQRGY
                     EALTLHEYVRMPFVHTARDSAVYINFDYLARVVRGTLATTLVAAETFAPGLLRFALPG
                     PLADPLPAGRPIELEVYLRAAGSGCPAAGGARLHYSFGEGAWDSLSMAESGADRYRAE
                     LPPVYEMTPLHFYFTATDTSGSVFAYHDREAPFLAVVAARSEVVFRDDFNQDCGWQVM
                     GGATEGVWERVLPQSAAFWGAPGRDYDNSGYCFLTGNATVEDVDGGTTMLVSPWIAVG
                     AGEARIRYARWFADYYPEGEPYSDTFRVYVRPSPSQSFRLVETVGPVVQADGGWYARE
                     FMAGEIVATTGALQLRFDASDAGADSHVEAAVDDITVWRYTCDPAVLTASLPDWTAGV
                     PYSCSLAAAACCSLLVWTDKNDALAGSGLVLEPSGALSGVPLAAQAINLTAQAVDDSG
                     RVGERTFPFTINPVLEVATDWLPRGMVGAAYSRRLEAEGGTGGRVWRDRDLDLAGSGL
                     ALDSSGLLSGTPVAAGGYSFVAEVVDQVGARAVRELAVAVDESYVCGDVSGDGVGPNI
                     QDLSFLVSFLFRGGPPPPVPAAANVDGAGAPGADITVADLTYLVAYMFRSGSAPICAG
                     PSAR"
     gene            complement(8356..9828)
                     /locus_tag="PKM94_09710"
     CDS             complement(8356..9828)
                     /locus_tag="PKM94_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD66954.1"
                     /translation="MTSTAPAAGAAGGAANPGLFRQLASLPRPFWMANVIEMFERLAY
                     YGVRVVIPIYIAQADEPGGLHFTQADKGLIFMIWAIVQSLVPMVSGGFADRYGYKKTM
                     AFAFTVKIIGYLTMATQREFWPFLGGVCLLAFGTAVFKPGVQGTLVRTLNNKNSSIGW
                     GTFYMLVNIGGFLGPPLAHFFYGISWPAVFFGCAGIISLNYIMLFTYKEVHAGGEQKG
                     GPLHILAITARNFLNGKLVVFILIMSGFWLMFMQLFDMLPNFIVDWVNSASLVKTLGL
                     PDWMLQLNSDRAPQLSQEWMINANSGLIILLVVPLSWMVSRLRRVHSITIGVIISSVG
                     LVLAGFTTAGVFCILGIMVFSVGEMLASPKMNEYLGVIAPEGQKGLYMGYANVPLAVG
                     WGYGSLYGGQVYGTMGEKAKLAADYLAAKYQVLDVARTEAFAKLQEVSGMNAQEATDA
                     LWHAYHPYKLWYQFAAVGLLSAAAMVWYSWWVKRDEKANA"
     gene            9958..10242
                     /locus_tag="PKM94_09715"
     CDS             9958..10242
                     /locus_tag="PKM94_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOD66955.1"
                     /translation="MTDHAPDPVEYPIDGTLDLHQFAPEDTRAVVEEYLRACLEKRIY
                     ALRIVHGKGSGVKRRIVHALLARHPNVVRFRHEEGSGGSWGATVVDLKPD"
     gene            10256..10771
                     /locus_tag="PKM94_09720"
     CDS             10256..10771
                     /locus_tag="PKM94_09720"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD66956.1"
                     /translation="MEPYLREILWRQCGAAIDMLENAIAACPDEVWAAPDGPAKAGDC
                     WYLAYHTLFWLDLYLDGAAEGFAPPAPFGLEELDPAGRLPERAYTQDQLLAYLAHGRE
                     KCRAAVAGLTDQSARRPCVFRWGEVAYVELLLYTMRHVQHHAAQMNRLLREATGSAPH
                     WVGITRRPLSR"
     gene            complement(10888..17286)
                     /locus_tag="PKM94_09725"
     CDS             complement(10888..17286)
                     /locus_tag="PKM94_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD66957.1"
                     /translation="MADSCGDADTVSHTYNITINQPPTIALGADTALALCAPQPICLS
                     YAAGDPDAGDLVTETMVSGYGAIDTAANQICFTPTSGGLYQFIVQATDNCGDSDLDTA
                     LVLVSFGDVAAIDCPTGPINVSLCAADEVCQMLAVTPPTAAVTASFGTWAAGQLCFQA
                     DTSGTYHIQVVASEDCGADTCLLTFNVVIGAAASITCPEPQTESLCAADTVCVPVSIN
                     GGNLSVTVTPTGWYSAGNVCFVADTSGHYALTVIANTLCGADTCAFTVDVTINTPPTA
                     ADPSSPVDTFMCAAAQVCYQFAAADAEGDPLTWSRLSGSGTVSSSGLWCFTAAAGSHS
                     VTAVAADPCGAKDTVSLTYEVAINSAPVMALGNDTTVFQCAATAMCLPYAATDADGNI
                     TLIEILAGAPGAVLDQPNSRFCFTPAGAGSYTFILRVTDACGQSDRDTMVVTVGLNSS
                     PAVSLGADQTLFACAPAQICLPVASSDPDNNLSAVTLVSGPGTLVGNQVCFTPTGTGT
                     YQFIVRATDACGAVDEDTALVYYTLNSAPTADAGADQTLFLCAPAPICWPAAGSDPDG
                     NLASVTLTQGPGTFNGSQICFTPSADGSYLFVLRATDACGAADYDSATITVALNEAPV
                     CVAPSDTSIAQCAAQEVCLPAYATDANGNLHMCQIISGPGSLVNGQWCYTPASDQTVT
                     VVMRCTDSCGAYCESQFTVDFDVNGPPQIAFGADINTFQCQLAEICLPYTASDPEGLG
                     TTTITLVSGPGTLDQANSRVCYTPAAPGTATFIIGIADHCNRADYDTINVTVGLNVPP
                     AVNAGSDQDLFLCQTGAEICWPASVTDANNNLTSVNLIGPGTFDGDEICFNPTTSGDY
                     DFILEATDACGVYRADTVTITVTNNEPPTVQLPGDSSLFLCAPQEVCFDYAADDPNGG
                     RLTETMLSGFGTIDTAANRICFTPAAAGSYEFVVRVTDSCGASDRDTMVVTVAFGTFV
                     SIDCPSTPIAVSLCGPDEVCYLLGITPGSATVSTSHGTYSGGQLCFPADTTGTYAIEV
                     IASTDCNADTCILEFTVDIGAAAQISCPGPQTFDICTPDTVCVPVTVLMRSDTVTVSP
                     IGWYAGGNVCFFADTSGHYELTVIAATTCGADTCTVIADVSINTAPAADLPDPVDSFL
                     CASGQICFAFAAADADGDPLTWTRLSGPGTVTPAGVWCFTPTESGAYPVTVIVADPCG
                     AADTATMTYNLTFNTPPVVDLGGGKVNKLTLFQCDPSEQCLPYTVTDPDNNVVREELV
                     SGTATLDTAANELCFTAPASGTYRFIVRVEDACGAFDLDTLDVTVQINYAPTANAGAD
                     QQLFLCASTPLCWPAGCTDPDNNLDSCYLITPAGTYSGGQICFTPDTAGIYGFILRTV
                     DVCGEADEDTAFVAVEINSAPVCHLPNDTTIAQCLPAQVQLPVSATDVDGNLDRCEII
                     TGPGSIQSGAWVYTPTADQTVTVQVLCIDSCGTVCTGDFTVAFQVNEPPAVNLGPDTT
                     LFLCQPQQVCRPVAVTDDENNLQSTTVISPAGATLNGDQVCFATALGQPTYPVIVEAV
                     DACGRSRRDTSIVTVNFNRPPALTVTPSFTIYLDFSGEVCFSVAATDPDNNLTGVGVT
                     APAALAGNEACFWADTSGVYCFVVTAHDACNVAAVDTICVTVAIDECIHLRVEKTHNS
                     FQGNHEFVDIFQQGSGKEIGGFDLLLQYDPTALLFNNAVPGDLLEQCGWEYFTYRQGP
                     AGNCSGCPNGLVRIVAIAEYNNGANHPGCFLQDLTGSLATLDFVVSNDRNLECQYAPI
                     RFFWVDCGDNAVSSRTGDTLFISRKVFDFELNEMTSYTGTLPGFTGAPDVCITSGGPG
                     KPSTVRCVDFTNGGVDIVCADSIDARGDINLDGQMNTIADAVLLSNYFVYGLGVFTIN
                     VEGQIAATDVNADGLALSVADLVYLIRVVVGDALPYPKLDPNKSAEVQLALIDGVLEI
                     QRTDYEIGAIALILEGEAVPELHPEAKGMELRYSFDGTDTRVLVYNSQAAAALSGGKV
                     LRIGGAARIKTIDLGAFNGLALTAKVSSLPSRYALSQNFPNPFNPATTIEFALPKTTD
                     WELKIFNVLGQTVETFTGTQDVGYMKIVWDAGRYASGVYLYRLTAGEFTATKKMVLLK
                     "
BASE COUNT         2949 a         5890 c         5602 g         2870 t
ORIGIN      
        1 tcaaacccgg ccgggacgag ctccgccggc gccgccgggg cctccgccca ggacgccgcg
       61 gcgttgacgc agaagtcgtc gaagtcgctg ttggacttgg tgtagcctac tcccaccagg
      121 ccggcccggg ccgagaaggt gtaggagccg atcgacgtcg acccgaccat catcgagacc
      181 gtcccgctcg cagccacgga cacggtcacg tcataccaga ccccggtgct gaaagtgtag
      241 ctcgcatacg cccgcgtgta ctggcgcccg ctgaggatct cgccgacgta cacccggttg
      301 tagtaatcgt cgaactgcac gtagcggtag ttcgagctgt tggtgtaggc gaagatgatg
      361 cgggtgttgc gctgcgtgcg cccggtgttc atgcgcacct tgcacgtaat cgtcgccgtc
      421 gagaacgtcc cgaagggcga ggtcagccgg gcattcgagg tcgtcgagtt ccccttcagg
      481 tagtagttcg cggccgacca ggtcccgagc ttgttgctcc acccggtgta gttgccgtcg
      541 ttaaagtcgt cgcactgcgc ggtctcggtc gtgaccgtaa tgtagttggt cttcgtctcc
      601 gagtccgaac cgtacgcatt ggtcgccgtc agcgtcaccg tgtacgtccc cgccgatgtg
      661 taggtgtggg tcgggttctg ggcggttgac gtgccgccgt cgccgaaatt ccacgaccag
      721 ctcgtgggag tgttggtcga ggcgtcggtg aacacgaccg tcagcggcgc cgtccccgaa
      781 gtcggcgtgc cggagaaggc cgccaccggc gggtaggtcg gcggcgccga caccgtgatg
      841 tagccggtct tggtctcgga gtccgaaccg tacgcgttgg tcgccgtcag cgtcaccgtg
      901 tacgttcccg ccgaggcgta ggtgtgggtc gggttctggg cagtcgacgt gccgccatcg
      961 ccgaaattcc acgaccagct cgtgggattg ttggtcgagg cgtcggtgaa cacgaccgtc
     1021 agcggcgccg tgcccgaggt cggcgtgccg gagaaagccg ccactggcgg gttgttggtc
     1081 gagatcgaca gcagggcggc gttggcgtcc accaagccgt agccgtagta ctggtcccac
     1141 ccggcggcgc cgagatcctc ggccgtgttc tgcagggccg cccgcacatc ggccggggcc
     1201 atcgagccgc cgttcttggc gatcagcagg gcggccacac cggccacgtg cggcgacgcc
     1261 atcgacgtcc cctcgtagaa gtagtacccg aacgtcccgt agttggtccc gtcatgcgtc
     1321 tgctgcagca cgccgtcaac atagccgtcg ccgttctggt cgacgctcac gtcgccgccg
     1381 ggcgcacaga tgtcgatggt cgagccgtag ctcgtgtacg acgtgtacgt cttgttgtag
     1441 cggacggccg agacggagat gcactgggag taggcggccg gatactgggg cgtggaggtg
     1501 ccggcgttgc ccgcggcgca gacgatcgtc acgcccttgt tgtaggcgta gacgaccgcg
     1561 ttctggaggg tggacgagtt gtaggatccg cccaggctca tgttgatcac gtgggcgccg
     1621 ttgtcggcgg cgaacgtgac cccgttggcg atcgccgtgt acgaaccgct gccggaggca
     1681 tcgagcactt tcaccggcat gatgctgcag ttgaaggcca cgcccgtcac gccgaggttg
     1741 ttgttggtgg tctgggcgac cgtgccggcc acgtgcgtgc cgtgggcgca gtcgtcgttg
     1801 gggtggctgt cgttgttgat gaaatcatag ccgggcacga agctcgtccc ggccaggtcc
     1861 ggggcctgcg tgaagctgcc gtaggtctcg taggcgaccc cgcagtcgac caccgccacg
     1921 atcacgctgg gcgagccggt ggtctggtcc cacgccgccg gcatcttgat cagcggcatg
     1981 tgccactggt acgtgtagta gggatcgttc ggcgtcatga aggcgtgggc gatatagttt
     2041 ggctcggcat actcgacatc gggacgggcg ccgaaagccg ccaccatctc ctcaaccgtc
     2101 gtccccgccg ggatccgaag ctggtggaaa ccgatctggc ggttggttga gatcgtggtg
     2161 gccccgaggt cggcgagcgc ggcactgacc gcctggcggc ccacgccgtc cttgaacttc
     2221 acaactatct cgtccggaac gtactcatct ccggacggcg ggacgcatga ctgcccgacc
     2281 cgaaagtcct cggcgaagac gaaaccgcaa aggacagtca gcatcaccag cacgaacact
     2341 gctctcatgg tttgactcct tcttcgattg ggaggaacgc tgcccgcaaa ggagaccctt
     2401 tgctccgggc agccgggttt aatcaagtga cggttgcgaa caagggaata gtgtctgtcg
     2461 ggcgcaccac ctcctcacat gcaatgagag tccacgggca gattgcatga tgatccgcac
     2521 catacgcgaa aacaaagggc tgactcagcc tatagtcgtt gtgattactg aaaaaataca
     2581 gggcgggcgc gcccgagtca ataaaaaaaa gcggaccccc ttttccaggt tccccccgcg
     2641 cggcgggcct agtcgtgctc gccggctttg cgccagcgct cctgcgcctc gcgcaccacg
     2701 cgctccgcgg ccgcccggtc ctcccacccc tcgaccgccg tcttcttctc ctccaactcc
     2761 ttgtatacct ggaagaaatg ctcgatctct ttcaccaggt gcggcgggac ctcctccagc
     2821 gcccggatcc tgttccacat cgggtcgtgg atcggcacgc acaaaatctt ctcatccggg
     2881 cccttttcgt cctgcatgcg gaacagccct accggacggg catcgatcag gcatcccggg
     2941 aaagtcggct cccaggtgag cacgagagca tcgagcgggt cgcggtcgcc ccccagcgaa
     3001 ttgaggatga aaccgtaatc gctcgggtag tgcaccgccg aaaacagcat gcgatcgaga
     3061 cggaacagcc cgcgcgcctt gtcgtactcg tacttgttcc ggctcccctt ggggatctcg
     3121 accagcactt cgacagtgaa gtcattttcc atcatacggt tctccgagag agccctccgc
     3181 tctcagcctc cggcgggcag gcgccgctct attcgtattc cgcacacatc gcgcgccgca
     3241 cggaaccgac cggccccccc ggacccggtc ggcgccaatc cgcgccgacg cctcctacat
     3301 cgacttcgcc aacttcacat acgcctcgac ctcggccagc gcccggtcga ccgcgctgcg
     3361 ccagaaaccc tcgtcggcca gatccacccc caggtgagtc cgggcgagct cttcgcaggt
     3421 catgctgccg gtgtccgcca ggagcgcgcg gtagtccttc gcgaacttct tcccctcccg
     3481 cttggcccga tcgtagacgc cgccggcaaa gaggaacccg aacacgtagg ggaaattgta
     3541 gaagggcgcc gaggtcatgt agaagtgcag tttggatgcc cagaagagcg ggtggcaccc
     3601 cgaatcgtcc agcattcccg caaacgcctc cttctgcgcc gtcaccatca tctccgacag
     3661 ccgttccgcc gggaccatcc cgttccggcg ttccgcgtaa aaggctcggt cgaaaatgta
     3721 ccggcagtgc aggtcggtaa acatcacata ggccgactgg agtttctggt ccagcagcat
     3781 gagccgttcc ccggcgtcgg ccgcctggcc cagggccgca tcggtcacca gcgtttcgga
     3841 gaaaatcgaa gccgtctcgg ccagcggcat ggggtactgt tgcgcgaaat acggtttctc
     3901 ggtgatgacc tgctggtggt aggcgtggcc gagttcgtgg gcgagcgtga gaaggttctc
     3961 gaacgacccg ccgtaggtca tgaacacccg cgactggcgg accggtccga acccggtgca
     4021 gaaggctccg cccgctttgc cggcgcggtc ctcggcctcc acccagcggc tgtcgatcgc
     4081 ccggcggtaa aaatccgcca tgtcatcgga gaacgaccgc acctgggcca cgacgaaatc
     4141 gccggcctcc gcgaagctgt actgccgatc ggaccggccg gccggcgcga attcgtcgta
     4201 ccagcggaac cggtcgatcc ccagcagtct cttcttcgcg tcgacatagg cgcggagccg
     4261 ctttttctcc tccgcaatca cccgccacat ggtgtcgagc gtcgcctcct gcaggcgggc
     4321 gttgcgcagc ggttcgtaca tgagatccca gccgcggtac ttgtagagcg acaggcggaa
     4381 tcccgcctgg gcgttgagcg ccatcgccgc cagatcctga cggcgcttcc acccgttggt
     4441 catcgcctca aacgcccgcc gccgcacctc ccggtcgggg ctggacattt tggtcgcaat
     4501 ctgccccatc gagagcagct tcgtctggcc gccttcctcg aacggttccc gaaggtcgcc
     4561 ggccatcttg tcatacaact ggttccaggt gtgatagccg ttgaccgcca gatccagcgc
     4621 cagctcctct ttttccaccg gcattttccg gcgggcggtc tcgcgcacct cgttgaggaa
     4681 aaactccgtc ccgcgcagtt ccggggccgc cgtgagcagc tgccaggccg catccgactg
     4741 ctgcagagac atcgcctcga tccgggtcat gagcttcttc cactgggcga catcctcctc
     4801 tccttcggca tcggcggcgt gggcaagcgt gtcgctgaca ttctgggctt tcagacacga
     4861 tgcgaacgcc agcagcaact cgaggtcggc gaggatggac tggagggtgg aaatagccgc
     4921 cgcccactgg gggcggttct ccggggtgat ggcggcgggg agccggtcga ccgcggtcgc
     4981 ggccccggcg agatctttct tcactttctc gcggaacgct ttgtactcgg cggaggcgct
     5041 gcctcccggg aagatcgagt ccagatccca ggtgggcgcg gcggggatgt gggtggcggt
     5101 gtgggacata ttcgcggtct ccgattcgtg ggggttagag catttttcca agtatggtga
     5161 aggtaagaaa aaagcagggg aaaggcaagg cgccgctgcc cgcacccggc cgcattgcgc
     5221 cggccggact cacccgtcgg gcgaccctgc gccgctcccc aatgccgccc tctcaccgcg
     5281 ccgagggtcc cgcacaaatc ggcgcggaac cgctccgaaa catgtaggcc acaagatagg
     5341 tcaggtcggc gaccgtaatg tcggcccctg gcgcgccggc gccgtcaaca ttcgccgccg
     5401 ccggaaccgg cggcgggggc cccccgcgga agagaaacga cacaagaaac gagagatcct
     5461 gtatgttcgg tccgacgccg tcgccgctga catcgccgca gacgtaggac tcgtcgaccg
     5521 ccacggcgag ttctctgaca gcgcgggccc cgacctgatc aaccacttcc gccacgaacg
     5581 aatacccgcc ggcggcgacc ggcgtcccgc tcagcagccc cgacgagtcc agggccaggc
     5641 cgctgccggc caaatcaaga tcccggtcgc gccacacccg ccccccggtt ccgccctccg
     5701 cttcgagccg gcggctgtag gccgcgccga ccatgccgcg cggcagccaa tcggtcgcaa
     5761 cctcaaggac agggttgatc gtgaacggga acgtgcgctc cccgacccgc ccgctgtcat
     5821 cgaccgcctg cgccgtcaga ttgatcgctt gcgccgcgag cggcactccc gagagcgcgc
     5881 ccgagggctc caggaccagc ccgctgccgg ccagcgcgtc gttcttgtcc gtccacacca
     5941 gcaggctgca gcaggccgcc gccgccagcg agcaggaata ggggacaccc gcggtccaat
     6001 caggcaggga tgcggtcagc acggcgggat cgcaggtgta gcgccatacg gtgatgtcgt
     6061 ccaccgccgc ctcgacgtgg gagtccgcgc cggcatcgga ggcgtcgaac cggagctgca
     6121 gagcaccggt cgtcgcgacg atctccccgg ccatgaactc acgcgcgtac cacccgccgt
     6181 ccgcctgcac caccgggccg accgtctcca cgagccgaaa tgactggctg gggctgggcc
     6241 ggacatagac gcggaaggta tcgctgtacg gctcgccttc cggatagtag tcggcaaacc
     6301 agcgggcgta ccgaatccgc gcctcgcccg cgccgaccgc aatccaggga gacaccagca
     6361 tcgtcgtgcc gccgtccacg tcttcaaccg tggcgttacc ggtcaggaaa cagtacccgg
     6421 agttatcata gtcgcgtccg ggcgcccccc agaaggctgc cgactgcggc agaacccgtt
     6481 cccacacccc ttcggtcgcc ccgcccatta cctgccaccc gcaatcttga ttgaagtcat
     6541 cgcgaaagac gacctcggac cgggcggcaa ccaccgccag gaagggcgcc tcgcggtcat
     6601 gataggcgaa caccgacccc gaggtgtcgg tggccgtgaa gtagaagtgc agcggcgtca
     6661 tctcgtacac gggcggcagc tcggcgcggt agcgatcggc gccgctctcg gccatcgaga
     6721 gcgagtccca cgccccctcg ccgaacgagt agtgcagccg ggcgcccccg gccgccggac
     6781 agccggagcc ggcggcccgc agatacacct cgagttcgat cggacggccg gccgggagtg
     6841 ggtcggccag cggccccggc agcgcgaatc ggagcagccc cggcgcaaag gtctcggcgg
     6901 ccaccaacgt cgtcgccagc gtcccccgca caacgcgcgc gaggtagtcg aaattgatgt
     6961 acacggcgct gtcgcgcgcg gtgtgaacaa acggcatacg gacatactcg tggagggtga
     7021 gggcctcgta tccccgctgc tcgaacggct cggcatccca cgccgcgccg gccgccaggt
     7081 gcgccgagag gtcgaccccc gccagcgaat cggccaaggc tccccagagc gctccgtacg
     7141 aaccgggcac ggtgtgatag acccagacgg ccgagtcgtt ctcgtggtag ccgatcatgt
     7201 ccatgttgag cacaagcgcg atgctgtcct cccgccgcgc cgcctccgcg gcgtagtaat
     7261 cagagcccca caacccgcac tcttcagcgt cgaaagccgc gaacacgagc gacaccgggg
     7321 tctccagagc ggccgccacg cgcgcgattt cgagcacgcc ggcgatgccg ctcgcatcgt
     7381 cgtcggctcc cggcgactgc cgcggagagg aatcgcggtg ggcgccgatc agaatgtgaa
     7441 aatcgggata cagcgtcccc ggcttgacgg cgataacatt gaatgcggcc accgccggac
     7501 cgtctcccca gttctcgtag gcggcgaagc tgtctgtgat caccgagtca taaccgaacg
     7561 accggaactt gtcgacaagc cagtcgcgcg aggcggcatt ctggctcgtg cccaccttgc
     7621 gcgtcccgaa agcctgcagc cgctccacat aggaacggca ggaatcctca ttgatcagac
     7681 tgatcagcga gtcgagatcg agcgcgcgct ccggccgcgc gcggccgatc ggccgggccg
     7741 tgcggcagac aatcggcaga ctggtcgatg gcagcggcgc cagacccagc cagccgtttt
     7801 cgtccggcac aatccccccg cgcacgtcga cgagacgcaa ccccccgctc tcgaagacta
     7861 ccggatagcg ggcgcggtcc cccggatccg ttcgcaagtc gcgcgccaga tcgctccggg
     7921 cgatgttggc ccgaaccagc cgcgaccgga gcgcgctcgt ctccagagcg gccgcctgcg
     7981 gcgggtcgat gagcaccagg tagccgtcgg cgacccgggc caccgcatcg aggcccagac
     8041 cccggagcgc ttccgcgtcc gtcggaccgg tcaaggtaac gtgatagaga tctttccccc
     8101 ccgcaggggc ggccgcgcag agaatgagcg ccgcgataaa cgacaggcgg cggagggcca
     8161 gggcacccct gctgccgcgc cgtcctgaca gcgtgccggc catactcgca cctctcgccg
     8221 ggcggcccgg ctcggcttgc attcggttga acttctacgg cgatgactac ctatagtata
     8281 ctgcgcactc cctgtttcgt caagccctgt ccgggcgggc ggaggatcca ctccccggcg
     8341 gtgccctgtt ctcccctacg cgttggcctt ctcgtccctt ttcacccacc aactgtacca
     8401 caccatcgcc gccgccgaca acaggccgac cgcggcgaac tggtaccaga gcttgtaggg
     8461 gtggtaggcg tgccacaggg catcggtcgc ctcctgggcg ttcattcctg aaacctcctg
     8521 cagtttcgcg aacgcctcgg tacgggcgac atcgagcacc tggtacttgg cggcgaggta
     8581 gtcggcggcc agcttggctt tctcacccat ggtgccgtac acctgtccgc cgtagagcga
     8641 gccgtacccc cagccgaccg cgagcggcac gttggcgtac cccatgtaga gccccttctg
     8701 cccctcgggc gcgatcacgc cgaggtactc gttcattttc ggcgaagcga gcatctcgcc
     8761 caccgagaag accatgatgc cgagaatgca gaacacgccg gcggtcgtga atccggccag
     8821 cacgaggccg accgaggaaa tgatgacgcc gatggtgatc gagtgcaccc gccggagccg
     8881 cgagaccatc cacgacagcg gcacgacgag caggatgatc agccccgaat tggcgttgat
     8941 catccactcc tgcgacaact ggggcgcccg gtcggaattg agctgcagca tccagtcggg
     9001 cagcccgagc gttttcacca gcgacgcgga attgacccag tcgacaatga aattgggcag
     9061 catgtcgaac agctgcatga acatgagcca gaaccccgac atgatgagga tgaacacgac
     9121 cagtttgccg ttgaggaaat tgcgggcggt gatcgccagg atgtgcagcg gccccccctt
     9181 ctgctccccc cccgcatgca cttccttgta ggtgaagagc atgatgtagt tgagcgagat
     9241 gatcccggcg cagccgaaaa acaccgccgg ccaggagata ccgtagaaga agtgcgccag
     9301 cggcgggccg aggaaccccc cgatattcac cagcatgtag aacgtccccc acccgatcga
     9361 cgaattcttg ttgttcagcg tccgcaccag cgtcccctgc acccccggct tgaacaccgc
     9421 cgtcccgaac gccagcaggc acacgccgcc gaggaagggc cagaactccc gctgcgtggc
     9481 catcgtgagg tacccgataa tcttgaccgt gaacgcgaag gccatcgtct tcttgtagcc
     9541 gtagcggtcg gcgaagcccc ccgagaccat cggcaccagc gactgcacga tcgcccagat
     9601 catgaagatg agtcccttgt cggcctgggt gaaatgcagc ccgcccggct cgtcggcctg
     9661 ggcgatgtaa atgggaatca cgacccgcac tccgtagtag gccaggcgct cgaacatctc
     9721 gatgacgttg gccatccaga aggggcgcgg aagggaagcg agttggcgga aaaggccggg
     9781 gttggcggcc ccgcccgcgg caccggctgc cggcgcggtt gacgtcatgg tacctcctga
     9841 tgtatatttt ccagaccgct gcgccaatta acatgccggc ggggcgcaag gcaattgatt
     9901 catgcgcccc ggcagcttcc gcgccgcggc cgcaacccgc caccgggaga agaacctgtg
     9961 accgaccacg cccccgaccc ggtcgagtac ccgatcgacg gcaccctcga cctccatcaa
    10021 ttcgcccccg aggacacccg cgcggtagtc gaggagtacc ttcgcgcctg cctggaaaag
    10081 cggatctacg ctctgcggat tgtgcacggc aaggggagcg gggtcaaacg ccgcatcgtc
    10141 cacgccctcc tcgcccgcca ccccaacgtt gtccgcttcc gccacgagga gggttccggg
    10201 ggatcatggg gcgccacggt ggtcgacctg aagccggatt gacaaggtgg aggctgtgga
    10261 accgtatctg agggagattc tctggcgcca gtgcggagcc gccattgaca tgctcgagaa
    10321 tgccatcgcc gcctgcccgg acgaggtctg ggcggcgccg gacgggcccg ccaaggccgg
    10381 ggactgctgg tacctcgcct accacaccct gttctggctt gatctctatc tcgatggtgc
    10441 ggcggagggg ttcgcgccgc cggccccgtt cgggctcgag gagctcgatc cggctggacg
    10501 gcttccggag cgggcctaca cgcaagacca actgctggcc tacctggcgc acggccgcga
    10561 gaagtgccgc gccgccgtcg ccgggctgac cgatcaatcg gcgcgccgcc cctgcgtctt
    10621 tcgctggggc gaggtcgcat acgtggaact gctgctgtac acgatgcgcc acgtgcagca
    10681 ccacgcggcc caaatgaacc gcctcctgcg cgaggcaacc ggctcggctc cgcactgggt
    10741 cgggatcacc cggcggccgc tcagccggtg agcgccgcct gcgtcccggc cgccgccaac
    10801 gacaaacccc gcgggcagta ggcgcggggt ttgtcgttta tgaagggtcc ccctctcggg
    10861 ttaccctccg ggtcggtggc cctgccgcta cttcagcaga accatcttct tcgtggcggt
    10921 gaactcacca gccgtcagac gatagagata cacccctgag gcgtatcgtc cggcatccca
    10981 cacaatcttc atgtacccga cgtcctgggt ccccgtgaac gtctcgaccg tctggccgag
    11041 cacgttgaag atcttgagtt cccaatcggt cgtcttgggc agggcgaatt cgattgtcgt
    11101 cgccgggttg aacgggttcg ggaagttctg cgagagcgcg taacgcgacg gcaggctgct
    11161 caccttggcc gtcagcgcga ggccgttgaa ggccccgagg tcgatcgtct taatcctcgc
    11221 cgcccccccg atccgcagga ccttcccgcc ggagagcgcg gccgccgcct ggctgttgta
    11281 gacgagcacg cgggtgtcgg tcccgtcgaa gctgtaccgc aactccatcc cctttgcctc
    11341 cgggtgcagt tccggcacgg cctcgccttc gaggatcagg gcgatggcgc cgatctcgta
    11401 gtcggtcctc tggatctcca gcacgccgtc aatcagcgcc aactgcacct cagccgactt
    11461 gttgggatcg agtttcggat acgggagcgc gtcgccgacc actacccgga taaggtagac
    11521 caaatcggcc accgacagcg ccagcccgtc ggcgttcacg tccgtggccg cgatctgccc
    11581 ctccacgttg atcgtgaaca cgcccaggcc gtagacgaaa tagttgctca ggagcacggc
    11641 gtcggcgatc gtattcatct ggccgtcgag gttgatgtcg ccgcgggcgt cgatcgagtc
    11701 ggcgcacacg atgtccacgc cgccgttggt gaagtccacg cagcgcacgg tcgagggctt
    11761 gccgggaccg ccgctcgtga tgcagacgtc cggcgccccc gtgaaccccg gcagcgtccc
    11821 ggtgtacgag gtcatctcgt tgagctcgaa atcgaacacc ttgcgcgaga taaagagcgt
    11881 gtcgccggtg cgcgacgaca ccgcgttgtc gccgcaatcc acccagaaga accggatcgg
    11941 cgcatactgg cactcgaggt tgcggtcgtt ggacacgacg aagtcgagcg tcgccagcga
    12001 accggtcaga tcctgcagga aacagccggg gtggttggcg ccgttgttgt actcggcgat
    12061 cgccacaatc cgcaccaggc cgttgggaca gccggagcag ttgcccgccg gaccctggcg
    12121 gtaggtgaag tactcccagc cgcactgctc gagcaggtct ccgggcacgg cgttgttgaa
    12181 caggagagcc gtgggatcat actgcagcag caggtcgaac ccgccgatct ccttgcccga
    12241 tccctgctgg aagatgtcga cgaactcgtg gttgccctgg aagctgttgt gggtcttctc
    12301 cacccggagg tggatgcact cgtcgatcgc caccgtcacg cagattgtgt cgacggcggc
    12361 aacgttgcag gcatcgtgcg ccgtcacgac aaagcagtac acgcccgaag tatcagccca
    12421 gaagcaggct tcgttccccg ccagcgccgc cggcgccgtc accccgaccc cggtgagatt
    12481 gttgtccggg tcggtcgccg cgacgctgaa gcacacttcg cccgagaagt cgaggtagat
    12541 cgtgaaactc ggcgtcacgg tgagcgccgg cgggcggttg aagttgaccg tgacaatgct
    12601 ggtgtcgcgc cgcgagcggc cgcaggcatc gaccgcctcg acaatgaccg ggtaagtcgg
    12661 ctggccgagc gcggtcgcaa aacagacctg gtcgccgttg agcgtcgccc cggccggcga
    12721 aatgaccgtc gtgctctgca ggttgttctc gtcatcggtc accgccaccg ggcggcacac
    12781 ctgctggggc tggcagagga acagcgtcgt gtcgggtccg aggttcaccg ccggcggctc
    12841 gttcacctgg aaagcgaccg tgaagtcccc cgtgcacacc gttccgcacg agtcgatgca
    12901 cagcacctgc accgtcaccg tctggtcggc cgtgggcgtg tagacccagg cgccgctctg
    12961 gatggagccg gggccagtga tgatctcgca ccggtcgagg ttgccgtcga cgtcggtcgc
    13021 gctcaccgga agctggacct gcgccggcag acactgcgcg atggtcgtgt cgttcggcag
    13081 gtggcacacc ggcgcgctgt tgatctcgac cgccacgaac gcggtgtcct cgtcggcctc
    13141 cccgcacacg tcaaccgtcc gcaggatgaa cccgtagatg ccggccgtgt cgggcgtgaa
    13201 acagatctgc ccgccgctgt acgtccccgc gggcgtgatc aggtagcagc tgtcgaggtt
    13261 gttgtccggg tccgtgcagc ccgccggcca gcagagcggc gtgctcgcgc acaggaacaa
    13321 ctgctggtcc gccccggcgt tcgccgtcgg cgcgtagttg atctgcaccg tcacgtcgag
    13381 cgtatccagg tcgaatgcgc cgcaggcatc ctcgacccgc acgatgaacc ggtaggtgcc
    13441 tgaggcgggg gccgtgaagc acagctcgtt ggccgccgtg tccagggtcg ccgtcccgct
    13501 gaccaactcc tcgcggacga cgttgttgtc cgggtcggtc accgtgtacg gcaggcactg
    13561 ctcgctggga tcgcactgga agagcgtgag cttgttgacc ttgccgccgc cgaggtcgac
    13621 cacgggcggc gtattgaatg tcagattata ggtcatcgtc gccgtatcgg ccgcgccgca
    13681 cggatcggcc acaatgaccg tgaccgggta cgcgccgctc tcggtcggcg tgaaacacca
    13741 taccccggcc ggcgtcaccg tccccggccc gctcaggcgc gtccaggtca gcggatcgcc
    13801 gtctgcatcc gcggccgcga acgcaaagca gatctggccc gaggcgcaga ggaaactgtc
    13861 gaccggatcg ggcaggtcgg ccgccggcgc cgtgttgatc gacacgtcgg cgatcaccgt
    13921 gcaggtgtcc gccccgcagg tggtcgccgc aatcaccgtc agttcgtagt ggccgctggt
    13981 gtcggcgaag aagcagacgt tgccgcccgc gtaccacccg atcgggctga ccgtcaccgt
    14041 gtcgctccgc atcaggaccg tcacgggcac acacaccgtg tcgggcgtgc agatgtcgaa
    14101 cgtttgcggc ccggggcagc tgatctgcgc ggccgcgccg atgtcgaccg tgaactcaag
    14161 gatgcaggtg tcggcgttgc agtcggtcga ggcaatgacc tcgatcgcgt aagtcccggt
    14221 cgtgtccgcc gggaagcaga gctgcccgcc gctgtacgtg ccgtgggacg tgctcaccgt
    14281 cgcgctgccg ggcgtgatgc cgagcaggta gcagacctca tccggtccgc agagcgagac
    14341 ggcgatcggc gtgctcgggc agtcaatcga gacgaaagtc ccgaacgcca ccgtcaccac
    14401 catcgtatcc cggtcgctcg caccgcacga atccgtcacc cgcacgacga actcgtagct
    14461 cccggcggcg gccggcgtga aacagatccg gttggccgcc gtgtcgatcg tgccgaaacc
    14521 cgagagcatg gtctcggtga gccgcccccc gttcggatcg tcggccgcgt agtcaaagca
    14581 gacctcctgc ggcgcgcaca ggaagaggct cgagtcgccc ggcagctgca ctgtcggcgg
    14641 ctcgttgttc gttactgtga tcgttaccgt gtccgcccgg tacaccccgc aggcatcggt
    14701 tgcctcgaga ataaaatcgt agtcgcccga ggtcgtcggg ttgaaacaga tctcgtcccc
    14761 gtcgaacgtc cccgggccga tcagattcac gctcgtcagg ttgttgttgg cgtcggtcac
    14821 cgacgccggc cagcagatct ccgcacccgt ctggcagagg aagagatcct ggtcgctgcc
    14881 ggcgttcacc gccggcggca cgttgagccc gacggtcacg ttgatcgtgt cgtaatcagc
    14941 ccggttgcag tggtcggcga tgccgatgat gaaagtcgcg gtgcccgggg ccgccggcgt
    15001 gtagcaaacc cgggagttcg cctggtcaag cgttcccggc ccggacacga gcgtgatggt
    15061 cgtcgtcccc agcccctccg gatcggaggc ggtgtagggc agacagattt cggcgagctg
    15121 gcactggaac gtgttgatgt ccgcgccgaa ggcgatctgc gggggaccgt tgacgtcaaa
    15181 atcgaccgtg aactgcgatt cgcagtaggc cccgcaggaa tcggtgcagc gcatcaccac
    15241 tgtcaccgtc tggtcgctgg ccggcgtgta gcaccactgg ccgttcacca gcgagcccgg
    15301 accggaaata atctggcaca tgtgcaggtt gccgttggcg tcggtcgcgt aagccggcag
    15361 acagacctcc tgcgccgcgc actgggcgat gctcgtgtcg ctcggcgcca cgcagaccgg
    15421 ggcctcattg agcgccaccg ttatcgtcgc cgaatcgtaa tccgccgccc cgcaggcgtc
    15481 ggtcgcccgc agcacgaaca ggtacgatcc gtcggcggac ggcgtgaagc agatttgcga
    15541 gccgttgaac gtccccgggc cctgcgtgag cgtcacgctc gccaggttcc cgtcggggtc
    15601 gctccccgcg gccggccagc agatcggcgc gggcgcgcag aggaagagcg tctggtccgc
    15661 tcccgcatcg gccgtcgggg cgctgttgag cgtgtagtac acgagcgccg tgtcctcgtc
    15721 aaccgccccg cacgcatcgg ttgcccgcac gatgaactgg tacgtccccg tgcccgtcgg
    15781 cgtaaagcac acctggttgc cgaccagggt gccggggccg ctgaccaggg tcaccgcgct
    15841 gaggttgttg tccggatcgc tgctcgccac cggcagacag atctgcgccg gtgcgcatgc
    15901 aaacagcgtc tggtcggctc ccagactcac cgccggcgag ctgttgagcc cgaccgtgac
    15961 caccatcgtg tcgcggtcgc tctgcccgca ggcgtccgtg acccgcagga taaacgtgta
    16021 cgaaccggcc ccggcgggcg tgaagcagaa ccggctgttg ggctggtcga gcaccgcccc
    16081 cggcgcgccg gcgagaatct ctatcagtgt aatgttgccg tccgcatcgg tcgccgcgta
    16141 cggcaggcac atcgccgtcg ccgcgcactg gaagaccgtc gtatcgttgc ccagcgccat
    16201 gaccggcgcg ctgttgatcg ccacctcgta ggtgaggctg accgtgtcct tcgccccgca
    16261 cggatcggcc gccaccgccg tgacgctgtg gctgccggcc gccgcggtga aacaccacag
    16321 acccgaggaa ctcacggttc cgctccccga aagacggctc caggtgagcg gatcgccctc
    16381 ggcgtcggcc gccgcgaact ggtagcacac ctgcgccgcc gcgcacatga aggtgtcgac
    16441 cggcgaggac ggatcggccg ccgtcggcgg cgtgttgatc gtcacgtcca ccgtgaacgc
    16501 acaggtgtcg gccccgcaca gcgtgttggc gatcaccgtc agcgcgtagt ggccgctcgt
    16561 gtcggcgacg aaacagacgt tgccggccga gtaccagccg gtcggcgtga ccgtgaccga
    16621 gaggttgccc ccgttgatgc tgaccggcac gcagaccgtg tcggccgcgc acagcgactc
    16681 cgtctgcggc tcagggcagg tgatcgaagc cgccgcgccg atgacgacgt tgaaagtgag
    16741 caggcaggtg tcggcgccgc agtcctccga ggccaccacc tgaatgtggt aggtcccgct
    16801 cgtatccgcc tggaaacaga gctggccggc cgcccacgtc ccgaacgagg ccgtcaccgc
    16861 cgccgtgggc ggcgtcaccg cgagcatctg gcagacttcg tcggccgcgc agagcgacac
    16921 gttgatcggc ccggtcgggc agtcgatcgc cgcgacgtcg ccgaaactca ccagcaccag
    16981 ggccgtgtcg aggtcgctgt cgccgcaatt gtcagtcgct tgcacgatga actggtagag
    17041 accgccgctc gtcggcgtga aacagatttg attggcggcc gtgtcgatcg cgccgtaccc
    17101 ggacaccatt gtctcggtca ccaggtcgcc cgcgtcagga tcgcccgccg cgtagctcag
    17161 gcagatcggc tgcggcgcgc acagcgccag ggccgtgtcc gcacccagcg cgatcgtcgg
    17221 cggctggttg atcgtgatgt tgtaggtgtg gctcaccgtg tcggcatccc cgcaggaatc
    17281 cgccacctgg gccgtcaccg cgtaggcgcc g
//
LOCUS       DAOPOE010000070        17076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4000_length_17076_cov_5.567182, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000070 DAOPOE010000000
VERSION     DAOPOE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_17076_cov_5.567182"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKM94_09730"
     CDS             <1..183
                     /locus_tag="PKM94_09730"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOD66958.1"
                     /translation="HLPGWSVEAIDQLGVDGDLAEAAAYAVMAAACVRGEPLSGARTF
                     GVARAIAGRIVQPPAG"
     gene            187..1470
                     /locus_tag="PKM94_09735"
     CDS             187..1470
                     /locus_tag="PKM94_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOD66959.1"
                     /translation="MIDRDNLRIKRLIRLTARVGTALCLMVLVWSCGRVPGLKDEEPG
                     SFIRVPFVRVLLGEDRPEATVSADHAFAIECLTAGEQHVFYSSRPVRIQSLGREVRVL
                     TRDGQIIQDRAGEVNIIPRGTGNRTMVDGRPYRGIIHLLPYANNLRIINIVYVEDYLR
                     GVVPPELGERADGEIEAVKAQAVAARTYAMSHLEQYGGEPYDLKASVYDQVYEGYGVE
                     NRLVNRAVDATAGVVATFEGKMINAYYHSTCGGMTDDIAHIWDRPSIPYLRAVADSGA
                     CGWSKYYRWREVYTEGQLRGRVEQYLSGDRGRDLRVGEIRNVAVAERTPGGRVSTLVI
                     ETEMDTYRFHKDRIRWVIGRTSNPDLILPSDRFDVILERTGEGRISRINFEGSGYGHG
                     VGMCQCGAIGMSRTGYTYEQILKRYYAGCELKQLY"
     gene            1470..2930
                     /locus_tag="PKM94_09740"
     CDS             1470..2930
                     /locus_tag="PKM94_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD66960.1"
                     /translation="MTSGLDQRARPRRTRWLVTMLLLALPAAGAARPYFIGARGELAT
                     LTGGHAPHFYVRGAYGLEAGLDLSPRWSLEASLSRYDLYNDTTIAGSVTLNGDREAAT
                     ARFSGTRLGVDAVATLFAPASWLSVRGGPGGGLLIWKMRDPDGDTVLKAVGGRGETID
                     FASTEIFVSGRLGIEVQLAAHLSTAITAHVDYLTGAGTDFADAVNARRDRWLGGVGAS
                     LRLTFGGERKAWASHEPWSAAAAPAPPRDLDSDGDGIPDREDRCAGTPAKVAVDPYGC
                     PLDTDGDGVVDSEDDCPGTEARARGRVDIHGCAVDADFDGVADFLDECPGNRVGAEVD
                     RRGCPVDDDGDGVPNGLDDCPHTLYGVPVDKFGCLDLALFDEPMVLNIDYPSGSFEID
                     PSSQERLRSLARALLVAPNVRLEINGYTDNIGTDEANRSLSEKRANRVRDFLAAQGVA
                     ESRMSVNGRGETGFVASNETAEGRALNRRIEIVFYK"
     gene            2936..3490
                     /locus_tag="PKM94_09745"
     CDS             2936..3490
                     /locus_tag="PKM94_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOD66961.1"
                     /translation="MYDHYLEQNIEDRRSARFALVVFLPPELDDHLAPLRERYDPLWS
                     LIPAHATIVFPFESRHSLDGLTGLIQEELAHEPVIPIRLGTIHDFYPRYPVICWKIQK
                     NEQLTGIYYRMYARLGMPIPFKEYQPHVTVAREISDHRVMLVKEKIVPYLSEEQFVAR
                     SIELVTPLPGNRWVSVRTFPLAGA"
     gene            complement(3588..4112)
                     /locus_tag="PKM94_09750"
     CDS             complement(3588..4112)
                     /locus_tag="PKM94_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOD66962.1"
                     /translation="MRKVSVSALILVSFMMLAFLAYAQETAEKKAFEYIGPAKCKMCH
                     KDVHAAWETTAHAKAFASLSAEEQKKAECNVCHMTGANAKGEAIENVTCESCHGPGSA
                     YSKPTIMTKKWSEDPATYKAKAIEAGLVYPDSALCATKCHKKEGNPNFKPFSWDKMSK
                     LVHPVAATPAEEKK"
     gene            4380..5594
                     /locus_tag="PKM94_09755"
     CDS             4380..5594
                     /locus_tag="PKM94_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD66963.1"
                     /translation="MKVLIVTQHYPPERGAVRRLAEFARQFVKAGMDVSVLTAVPNYP
                     DGIVPEKYRGKFFYRETVDGVKVYRSYVMPASNRFPGRRMVGFVIFLITALINSFRIK
                     GNFDLILASTPPVTTPVIGWVLSRVRRAKLIIEIRDLQPESSEEFGNLRPSLFTRMLK
                     KFMHMLYRRADHVVTATDGIANYLTGLGFAKERVTTIKSGVGREFLSSDSNGIRRKFG
                     WDEKFLVLYAGTLGWAHSLETVIEAARHLVDQPDIHFVFAGEGQKRQALEGMVRDYGL
                     KNVTFIGLQSLETIPYFLRASDVLVESLKEVPITKGTFPCKLFEYMASGRPIIFGSSG
                     GEAVSELEKAGGALWFSTDRPERLSELILQIKGGEIDGGRLGAAYQAHVDRYHRREVW
                     ADRYLEVIRSLS"
     gene            5682..7112
                     /locus_tag="PKM94_09760"
     CDS             5682..7112
                     /locus_tag="PKM94_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD66964.1"
                     /translation="MGLSSRPAAAPAQRLTVADLPGLLIGGETVAPTLRGPQRYVNFD
                     NAASTPTFRPVAEAVMEFLQWYANVHRGTGFKSQLASWAFEEARDIIAGFIRADLQRQ
                     VVIFTKNTTEAINKLARRLSTSKHDVILTTLMEHHSNELPWRRVGTVVHVGLNADGTI
                     SREDFLAKLGQFGDRVRIVAISGASNVTGYINDLDFFARETHRAGASILVDAAQLAPH
                     RPLDMKPGDPACALDYAVFSAHKMYAPFGIGVLVGHRDVFERGDPDTVGGGMVDIVTL
                     EEAYWTDLPEKEEAGTPNIVGVVALAKAIRMYEALGWEAIIAHEAELTAHALRRLGAI
                     PGVVLYGDREPERARARLGVVSFNVQGVPHALTAAILGSEAGIGVRSGCFCAHTYVKE
                     LLHVSPEAARELEYQILARDRSQLPGAVRVSFGLYNTTAEVDRLAEAVARIARGEYAA
                     GYVLNREKGEYAHPDLAPEFARYFDL"
     gene            complement(7512..8228)
                     /locus_tag="PKM94_09765"
     CDS             complement(7512..8228)
                     /locus_tag="PKM94_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66965.1"
                     /translation="MRYRKTRIDADSAERILDRALAARREAPDGLPTPLAFVRTRVEA
                     RLADSRKETLMARTLHTLGAHPRLSFGVAVGLAFILFGALVPLPYSSVIGYSVRITAP
                     ADINLAPQAYADALHAAGIANPSVNVTIDDAAAKYTIAGLSSESDARAAARTYTELTG
                     ADPDYAVIPIVQRTSGTLYAQAIQRLFRVEVAADGKSDEEIAAEIAAQIAAQGGEVKQ
                     VTINRGPDGRIDISIDIDTQ"
     gene            complement(8203..8754)
                     /locus_tag="PKM94_09770"
     CDS             complement(8203..8754)
                     /locus_tag="PKM94_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD66966.1"
                     /translation="MEHERFWQLLEPEHAKAQRFCRRLTGSIDDGDDLYQDGLLAALR
                     RFDSLRDPGAFRAWLYRILVNRYRNRHRWRRRQRTEALDDEVMTGPAHDPAGELAARR
                     WLDRALAALRPEERALVVLFELEGWTITELAANLGRPEGTIKARLARARRKMRRRIQT
                     YLPPKTTNPLPGKAGYALSQDQD"
     gene            9481..9825
                     /locus_tag="PKM94_09775"
     CDS             9481..9825
                     /locus_tag="PKM94_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOD66967.1"
                     /translation="MKYHEAAYCGDFCGGCEQYLKTCSGCDPKYKEECPFIACCRQHQ
                     AVHCGVCAEFPCTRLSAFVPDDRPGHPRGYHIENLRCRAIVGTADWLAVQRAKHGLPA
                     EGVAEADEGGGE"
     gene            9897..10673
                     /locus_tag="PKM94_09780"
     CDS             9897..10673
                     /locus_tag="PKM94_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOD66968.1"
                     /translation="MPTSKRERWSRDQLLIAFNLYCRLPFGRLHSRHPDVIRAAELIG
                     RSPDALAMKLVNFASLDPQHVARGVRGLNNVSRADRDMWREFSGNWEALAYESETAWR
                     ALLTGQDHGREGEEWEAPEGVTEKVMSTRVRVVQGFFRRTVLAAYEYRCACCRLSIQQ
                     MLNASHIIPWRVDKQRRADPTNGIAMCAFHDRAFDRGLITLDGDRRIVVSREVPTKSR
                     VAMHRVGLIELAGARAAAPSRFAPDEAALEYHRENIFIGG"
     gene            complement(10922..11686)
                     /locus_tag="PKM94_09785"
     CDS             complement(10922..11686)
                     /locus_tag="PKM94_09785"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD66969.1"
                     /translation="MLLAIDIGNTNVVFGLFRGADLVGDYRIATPPRSTPDEAGLSIT
                     HFLMRHRIEPARVEKVVIASVVPHLTPVFERTSRRYLSREPLTVSCRLKLPIAIRIES
                     PDQVGADRIANAVAALARWGGPAIVVDLGTATTFDVLDAAQAYIGGVIIAGPEAAMAE
                     LARRAARLFEVRIEKPDTVVGKSTAGALKSGAFYGLIGQVDFIIDRIIEECGFDRCKV
                     AATGGLAFEIEGHSRHITEVVPSLTLEGLRIIGEGN"
     gene            11845..12999
                     /gene="alr"
                     /locus_tag="PKM94_09790"
     CDS             11845..12999
                     /gene="alr"
                     /locus_tag="PKM94_09790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOD66970.1"
                     /translation="MTAPKLLSWIELSQSALRRNIESLRRLAGARMVAPSVKSNAYGH
                     GLTEIITMLRRSGGCEYVTVHSLEEALTCRRAGWTEGIMVLGPLPPGEYGQVIEQDLE
                     PAVFDLVTLRELGKLGDRKGAAIRTHLKLETGTNRQGFAAQDLPAVAGLYKRFRSLGR
                     PHGASMHFANIEDTTVHEYAEQQLRAYRDLLARLRRLGISPRLRHTASSAATILFEKT
                     RYDLVRPGISVYGHWPSKETYLSYRLQGGGNRLFEPVLTWKTRITQLKKVAADSFVGY
                     GCTYRTTAPTRLAVLPIGYYDGYDRSLSNRAYVLVHGRRAPVRGRICMNLMMVDVTDI
                     SGVRLHDEVVLIGRAGEERVSAEQIGDWAGTINYEVLARLSLSTPRLIVD"
     gene            13205..13879
                     /locus_tag="PKM94_09795"
     CDS             13205..13879
                     /locus_tag="PKM94_09795"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOD66971.1"
                     /translation="MNVSSKTGLKKKISESTIHRLSLYCRALSLLEKENYETVSSKEL
                     AKREKLTPAQVRKDLSFFGSFGTRGLGYPVNELKQRIASILGIDRVWRVALVGVGNIG
                     SALVSYKEFMRQGFHIVKLFDNDQRKIGSNHKGIIVSDIANLEKELKEAGIEIVVLAV
                     PATVAQYIVDDVVRAGVRAVLNFAPVNLRVPPDVYLRNENMSMELEYLSFALVNNPPK
                     KASKQG"
     gene            14068..15462
                     /locus_tag="PKM94_09800"
     CDS             14068..15462
                     /locus_tag="PKM94_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOD66972.1"
                     /translation="MRRSLRRLLRTALPPGIVLGALVSATGGWSQESAVWGDHALGTR
                     SYDDYATPDYCGTSCHVDFYRQWEQAMMAQAYVHHWDEIEYFKLAVPHAEKDAKVAGV
                     KAGCNGCHAPLAFLAGDVPPPRPEAGSRANEAVSCEVCHSIRGYAGDTPYNFNWITDP
                     GDRKYGPRPADQVAESPAHKMAPSEFIRTAEFCGTCHNEMSPYGVWVKSTHLEWAAGP
                     YAAQGVRCQDCHMTYAPGQAAAMGNEHSNVAQHLFAGAHVPAKVKGTIELTVFPSVRE
                     AVMGDPVEFKVALFNQKTGHKFPSGSVEDRIVWLHVEAVDAAGTAYHLPVDEKGFAGE
                     EYTIGADVRAYQDMGIALGQPDFPGVARDGVPAGDRIFRMPYFDPQGRMTIQQWNTAS
                     LGVDYRIGPRETKIETFTFTVPYEAAEGELTVTATLYYQRLVKPVADFLEVPAEEAEP
                     IVVSEYSTHITVIP"
     gene            15473..16675
                     /locus_tag="PKM94_09805"
     CDS             15473..16675
                     /locus_tag="PKM94_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66973.1"
                     /translation="MRRLLPVLLIGAAGALALAALFPAGEAAAIPAFARKYRMSCTTC
                     HQPFARLKAYGDDFAGNGFRLADKEAPGYFVETGDPNLSLIRDFPLSARIEGWARHET
                     GTRRETDFSTPYNVKLMSGGALGDHLAYYFYFLLSERGEVAGVEDAYLMFNDLFGQDL
                     DLYVGQFQISDPLFKRELRLTFDDYAVYVASPGASGINLKYDRGLMVTYGAPTGTDVA
                     VELVNGSGLAEGEGNRHVYDRDKYKNLLARVSQNLNEYLRAGIFGFYGKEADEYDRRN
                     EVWMAGGDATVGWGPAELNLQYVERRDDNPGFSLPADANEVETRGGFAELIVWPKGDR
                     SRWYGAAMFNYVESGLSMEPFAQKMYRVVTGHVGYLLQTNLRLTLENSYDLEAEENSL
                     LAGFVSAF"
     gene            complement(16798..>17076)
                     /locus_tag="PKM94_09810"
     CDS             complement(16798..>17076)
                     /locus_tag="PKM94_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66974.1"
                     /translation="KATVNSGGKPGNFRVYSRGTRFTMGQSSEFTGAVIAPNVNFNLA
                     QYTDFYGSLMSNRIDLSNNPRFHYDRSLGLIINGTTGRMIQVAWRELE"
BASE COUNT         2966 a         4788 c         6356 g         2966 t
ORIGIN      
        1 catctccccg ggtggtcagt tgaggcgata gatcagttgg gagtggacgg cgacctggcg
       61 gaggcggcgg cctacgcggt gatggcggcg gcgtgcgtac gcggggagcc tctctccgga
      121 gcgaggacat tcggtgtcgc tcgggcaatc gcggggcgca tcgtgcagcc gccggcgggg
      181 tgacgaatga ttgaccggga caacctcagg atcaaaagac tcatccgcct cacggcccgc
      241 gtcgggaccg ctctctgcct catggtgctc gtgtggagct gcggacgggt gccggggctc
      301 aaggatgagg agccgggctc atttatccgc gttccctttg tccgggtgct gctgggggag
      361 gaccgcccgg aggcaacggt ttcggcggac cacgctttcg ctatcgagtg cctcacggcc
      421 ggggagcagc atgtctttta ctcctcgcgt ccggtgagga tacagagcct gggccgcgag
      481 gtgcgggtgc tgacgcggga cgggcagata atccaggacc gcgcggggga agtgaacatc
      541 attccgcgcg ggacggggaa tcggacgatg gtcgacggtc ggccgtaccg gggaatcatc
      601 cacctactcc cctacgccaa caacctgcgc atcatcaaca tcgtgtacgt cgaggactac
      661 ctccgggggg tggtgccgcc ggagctggga gagcgggcgg acggggagat cgaggcggtg
      721 aaagcgcagg cggtggcggc ccggacctac gccatgtccc acctcgagca gtacggcggg
      781 gagccgtacg atctgaaagc cagcgtctat gaccaggtgt acgagggcta cggcgtggag
      841 aaccggctgg tgaaccgggc ggtcgacgcg acggcgggtg tcgtggccac tttcgagggc
      901 aagatgatca acgcctacta ccattcgacg tgcggcggaa tgaccgacga catcgcccac
      961 atctgggacc gcccgagcat tccctacctg cgggcggtgg ccgacagcgg ggcgtgcggg
     1021 tggtcgaagt actaccggtg gcgcgaggtg tacacggagg ggcaactgcg cggccgggtg
     1081 gagcagtacc tctcggggga ccgcggccgg gaccttcggg tcggagaaat ccggaacgtg
     1141 gcggtggcgg agcggacacc gggggggcgg gtctcgaccc tggtgatcga gacggagatg
     1201 gacacgtacc gtttccacaa ggaccggatc cgctgggtga tcgggcgcac ctcgaatccc
     1261 gatttgatct tgccgtcgga tcgcttcgac gttatactcg agcggaccgg ggagggccga
     1321 atcagccgga tcaatttcga gggcagcggc tacggccatg gggtcggcat gtgccaatgc
     1381 ggggcgatcg gcatgtcccg cacagggtat acgtacgaac agattctcaa gcgctactat
     1441 gccggttgtg aactcaagca gttgtactga tgacgagcgg acttgaccag agggcgcggc
     1501 cgcggcgaac gcggtggctg gtgacgatgc ttttgctggc gctgccggca gccggggcgg
     1561 cgcggccgta tttcatcggg gcccgggggg agctggcgac gctcacaggc ggacacgcgc
     1621 cgcacttcta cgtgcgcggg gcgtacggcc tcgaggcggg cctggacctg tcgccgcgct
     1681 ggtcgctgga ggcaagtctc agccgctacg acctctacaa cgacacgacg atcgcgggga
     1741 gcgtaaccct gaacggcgac cgggaggctg cgacagcgcg gttctcgggc acgcggctgg
     1801 gcgtggatgc ggtggcgacc ctgttcgcgc ccgcctcctg gctgagcgtc cggggcggcc
     1861 ccggtggggg cctgctcatc tggaagatgc gcgatccgga cggcgacacc gttttgaagg
     1921 cggtcggcgg gcggggcgag acgatcgatt tcgcctcgac cgaaatcttc gtatcggggc
     1981 ggctggggat cgaagtgcag ctggcggccc atctgtcgac ggcgatcacg gcgcatgtcg
     2041 actacctgac cggggcggga acggatttcg ccgatgcggt gaacgcgcgc cgggaccgct
     2101 ggctgggggg agtcggggcg agcctgcggc tgacgttcgg gggagaacgc aaggcatggg
     2161 cgtcgcacga gccgtggtcc gcggcggcgg cgcccgcacc gccccgggat ctcgacagcg
     2221 atggcgacgg aattcccgac cgggaagacc gctgcgcggg cacgccggca aaggtggcgg
     2281 tggatccgta cggctgtccg ctggacaccg acggggatgg ggtggtggac agcgaggatg
     2341 actgtcccgg caccgaggcg cgggcccggg ggcgggtgga tatccacggc tgcgcggtcg
     2401 atgcggattt tgacggggtg gcggacttcc tggacgaatg ccccggcaac cgggtggggg
     2461 cggaggtcga ccgccggggg tgtccggtgg atgatgacgg ggacggagtg ccgaacggcc
     2521 tcgacgactg cccgcacacg ctctacggag taccggtgga caaattcggc tgtctcgacc
     2581 tggcgctgtt cgatgagccg atggtgttga atatcgatta tccctcgggg tcgtttgaga
     2641 tcgatccttc gagccaggag cggctgcgga gcctcgcccg ggcactcctg gtggcgccga
     2701 atgtccggct ggagatcaac gggtacacgg acaacatcgg aaccgacgag gcgaaccgga
     2761 gcctgtcgga gaagcgggcc aaccgggtgc gggattttct cgcggcccag ggggtggcgg
     2821 agagtcgcat gagcgtcaac ggccgggggg agaccggctt tgtcgcctcg aacgagacgg
     2881 ccgaggggcg ggcgttgaac cggagaattg agattgtctt ctacaagtag acggtatgta
     2941 cgatcattac cttgagcaga acatcgaaga ccgacgcagc gcgcggttcg ccctggtcgt
     3001 gttcctcccc ccggagctgg acgaccatct cgccccgctg cgggagcggt acgatccgct
     3061 gtggagcctg atccccgcgc acgcgacgat cgtctttccg ttcgagtcgc gccactcgct
     3121 ggacggactg accggcttga tccaggagga gctggcgcac gagccggtga ttccgatccg
     3181 gctggggacg atccacgatt tctacccgcg gtatccggtg atctgctgga agatccagaa
     3241 gaacgagcag ctcaccggca tttactaccg catgtacgcg cggctgggga tgccgattcc
     3301 gttcaaagag tatcagccgc acgtgacggt ggcgcgggaa atctcggatc accgggtgat
     3361 gctggtgaag gagaagatcg tgccgtacct gtcggaggag cagttcgtcg cccggagtat
     3421 cgagctggtg acgccgctgc cggggaaccg gtgggtctcg gtgcggactt ttccgctggc
     3481 cggggcgtga ggcgccgggg cgaggcccgg gagaaaggca atgagaaggc gactcgccgg
     3541 ggggcgagtc gccttcatcg ttaactcaac agccgatcgg aagcggatta cttcttctct
     3601 tcagccggcg tggcggccac cgggtgaacc agcttggaca tcttatccca gctgaacggc
     3661 ttgaagttcg ggttgccttc cttcttgtga cacttggtgg cgcagagggc ggagtccggg
     3721 tagacgaggc cggcttcgat cgccttggcc ttataggtgg ccgggtcctc gctccacttc
     3781 ttggtcatga tggtcggttt ggagtaggcc gagcccgggc cgtggcagga ctcgcaggtg
     3841 acgttttcga tcgcctcgcc cttggcgttg gcgccggtca tgtggcagac gttgcactcg
     3901 gccttcttct gctcctcagc ggagagggag gcaaaggctt tggcgtgggc ggtcgtttcc
     3961 caggcggcgt ggacgtcctt gtggcacatc ttgcacttgg ccgggccgat gtactcgaag
     4021 gctttctttt cggcggtttc ctgggcatag gccaggaagg cgagcatcat gaaagacacc
     4081 aggatcagag cgctaacaga gaccttacgc atttgtgctt ctcccttggt tgggttccgt
     4141 ttcccagttt atacaccaat caatactatt cggatcgtga tttttgtcac atgggcgaaa
     4201 atatcatgtg aatgtcatgc tgtcaagttt ttcggatgaa tttggtcggg gaatttccga
     4261 tacatgacgg gggcggcggc cggggttgga ggggggttcc tgtgccgaag ggccaagctg
     4321 gtggagaaaa aaaacttgcc aggttctcca aaaagctgta gactgcgccg ggttagtcta
     4381 tgaaagtcct gattgtcaca caacactatc cgcccgaacg cggggctgtg cgacggttgg
     4441 cggagtttgc ccggcagttt gtcaaggcgg ggatggacgt gtccgtgctc acggccgtcc
     4501 cgaattatcc ggacggcatc gtccccgaga agtaccgggg gaagtttttc taccgggaga
     4561 ccgtcgacgg ggtcaaggtc taccggagct acgtgatgcc ggcctcaaac cggtttccgg
     4621 gccggcggat ggtgggattt gtcattttcc tcatcaccgc cctgatcaac agcttccgga
     4681 tcaaggggaa tttcgacctg atcctggcct cgacgccgcc tgtgaccacg ccggtgatcg
     4741 ggtgggtgct ctcgcgggtg cggcgggcga aactgatcat cgagatccgg gacctgcagc
     4801 cggagtcgag cgaggagttc ggcaatctgc ggccgtcgct cttcacgcgg atgctcaaga
     4861 agttcatgca tatgctctac cgtcgggcgg accatgtggt gacggccacg gacgggattg
     4921 ccaactacct gaccgggctg ggttttgcca aagagcgggt gaccacgatc aagtcggggg
     4981 ttgggcgcga gttcctgagc agcgactcga acggtatccg gcggaagttc gggtgggatg
     5041 agaagtttct tgtgctctac gcggggacgc tgggctgggc gcattcgctg gagacggtga
     5101 tcgaggcggc ccggcacctg gtcgaccagc ccgacattca tttcgtgttc gccggggagg
     5161 ggcagaagcg gcaggcgctc gaggggatgg tgcgggacta cggcttgaag aacgtgacct
     5221 ttatcggttt gcagtcgctg gagacgatcc cctatttcct gcgggcctcg gacgtgctcg
     5281 tggagagcct caaggaggtg ccgatcacga aggggacgtt cccgtgcaag ctgttcgaat
     5341 acatggcgtc gggccgcccg atcattttcg gctcgagcgg gggggaggcg gtgtcggagc
     5401 tggagaaggc gggcggggcg ctctggttct ccacggatcg tccggagcgg ttgtccgagt
     5461 tgatcctcca gatcaagggg ggggagatcg acggcggccg gctcggggcg gcctaccagg
     5521 cccatgtcga ccggtatcac cgccgcgagg tgtgggcgga ccgctacctt gaggtgatcc
     5581 gcagtctctc ctgacatttc ccgtccggta gcgaattctc tccccgttcc gtcgcggtat
     5641 aatgaactaa tcggacctat ttttatcaga ggatgcacac catggggctt agttcccgtc
     5701 cggcggccgc gccggcgcaa cggctgacgg tcgccgacct tcccgggctg ttgatcgggg
     5761 gggagacggt cgcgccgact ttgcgggggc cgcagcggta tgtcaatttc gacaacgcgg
     5821 cctcaacgcc gacgtttcgg ccggtggccg aggcggtgat ggagtttctg cagtggtacg
     5881 ccaacgtgca ccggggcacg gggttcaaat cgcaattggc gagctgggcg ttcgaggaag
     5941 cgcgggacat catcgcaggc ttcatccggg ccgacctgca gcggcaggtg gtcattttca
     6001 ccaagaacac gaccgaggcg atcaacaaac tggcccgacg gctgtcgacg agcaagcacg
     6061 acgtcatcct caccaccctc atggagcacc actccaacga gctgccgtgg cggcgggtgg
     6121 ggacggtagt gcatgtgggg ctgaacgccg acgggacgat cagccgggag gatttcctgg
     6181 ccaagctggg gcagttcggc gaccgggtgc ggatcgtggc gatcagcggg gcgtcgaacg
     6241 tgacggggta catcaacgac ctggatttct ttgcgcgcga gacgcaccgg gcgggggcct
     6301 cgattttggt cgatgccgcg cagctcgccc cgcaccgccc gctcgacatg aagccaggcg
     6361 atccggcgtg cgcgctggac tacgcggtgt tttcggcgca caagatgtat gcgccgttcg
     6421 ggatcggagt gctggtgggg caccgcgacg tgttcgagcg cggcgacccc gacacggtcg
     6481 gcggcggcat ggtcgacatc gtgacactcg aggaggccta ctggactgac cttccggaaa
     6541 aggaggaggc ggggacgccg aacatcgttg gcgtggtggc gctggcgaaa gcgatccgga
     6601 tgtacgaggc gctggggtgg gaagcgatca ttgcgcacga ggccgagctg acggcccatg
     6661 ccctgcgccg gctcggggcg attccgggag tggtgctcta cggcgaccgg gagccggagc
     6721 gggcgcgggc gcggctcggg gtggtgtcgt tcaacgtgca gggcgtcccc cacgcgctga
     6781 ccgcggccat tctcggttcg gaggcgggga tcggggtgcg ctccgggtgc ttctgcgcgc
     6841 acacgtatgt caaggagctc ctccacgtgt cgccggaggc ggcccgggag ctggagtacc
     6901 agatcctggc gcgggaccgg tcgcagctgc cgggggcggt gcgggtgtcg ttcgggctgt
     6961 acaacacgac cgccgaggtg gaccggctgg cggaggcggt ggcgcggatc gcgcgggggg
     7021 agtacgccgc ggggtacgtg ctcaaccggg agaaggggga gtacgcgcat ccggacctgg
     7081 cgccggagtt cgcgcgctat ttcgatttgt gagccgacgg tgcggggcgg cgcgagggcc
     7141 ggttttcgaa atttcgaaaa ccggctttgg cgggaaaggg cgttttcgaa aattcgaaag
     7201 cggcgggggt gtttttcgaa aattggaaaa cgggggttgg cgcggtcgat gatccgtttc
     7261 gcctagagcc gcgcggcgaa acgcggggcg gttgttcgac atcgcccgtt cggtccagca
     7321 gcgcggcggg cggctgaggg ggcattctct tcggcgaaaa ctctcacgac gaatcctcgg
     7381 gtcgatgggg agcgtgcagg ggcgtctttc gctttttcga aaatgaggtt ttcgggacag
     7441 ggcggacctc gaagtccgcc ccgttctgct gcgcggacgg cgctgtcgaa ctctcggcgg
     7501 cggtgcttga cctactgggt atcgatgtcg atgctgatgt cgatgcggcc gtcgggaccg
     7561 cggttgatgg ttacctgttt gacctcgcct ccctgggcgg cgatctgggc cgcgatttca
     7621 gcggcgatct cctcatcgga cttgccgtcg gcagccacct cgacccggaa gagcctctgg
     7681 atcgcctggg catagagcgt gcccgacgtg cgctggacga tggggatgac ggcgtagtcg
     7741 gggtcggcgc cggtcagctc cgtgtaggtg cgggcggcgg cccgggcgtc gctttccgac
     7801 gagaggccgg cgatcgtgta ttttgccgca gcgtcgtcga tggtgacgtt cactgaggga
     7861 ttcgcgatgc cggcggcatg cagagcgtcg gcgtaggcct gcggggcgag gttgatgtcc
     7921 gcaggcgcgg tgatgcggac ggagtagccg atcacgctgc tgtagggcag cgggacaagg
     7981 gcgccgaaga ggatgaaagc aagcccaacc gcgacgccga aactgagtct cgggtgagcg
     8041 ccgagggtat gcagagtccg agccatgagt gtctccttgc gggaatcggc cagacgcgcc
     8101 tcgacccgtg tccgcacaaa agcgagcggc gtcggcaaac cgtcgggcgc ctcccgccgg
     8161 gcggcgagcg cccggtcgag gattcgctcg gcagagtcgg cgtcaatcct ggtcttgcga
     8221 taacgcatat ccggcctttc ctggtaacgg gttggtcgtt ttcggcggga ggtacgtctg
     8281 gattcgccgg cgcatcttcc gccgcgcacg ggcgagccgg gccttgatgg taccttcggg
     8341 tcggcccagg ttcgccgcga gttcagtgat ggtccagccc tcgagctcga agagcacgac
     8401 aagggcgcgt tcctcgggtc ggagggcggc cagcgcgcga tccagccagc gccgcgcggc
     8461 gagttcgccg gcgggatcgt gtgcgggccc ggtcatgacc tcgtcatcga gcgcctcggt
     8521 ccgctgccgc cgccgccagc ggtggcggtt gcggtagcgg ttgaccagga tccggtacag
     8581 ccaagctcgg aaggcgccgg ggtcgcgcag cgaatcgaac cggcgcaggg cggccaggag
     8641 gccgtcctgg tacaggtcgt cgccgtcgtc aatcgagccg gtcaaccgtc ggcaaaagcg
     8701 ctgggctttg gcgtgttccg gttccagcag ctgccagaag cgttcgtgtt ccatggttct
     8761 gccgttacga caccgctcgg cgcggttcgg ttgcatacgg cggaaatata cacggttttg
     8821 gcgaagagcg ggtttcgaat attcgaaaac ggggtcgggg cgcgccggcc gggctgttca
     8881 acgtttgcac agcagggccg ttttcgaaaa ttagaaaacg cgaattgatt ttcgacgggg
     8941 gctgttttcc cttgtgcggc gcgggcaaac gggcggccga catttgcgaa aaggttgaag
     9001 actcagcaag tccacgagcc gcgggtggcc ggccgcgggg cgccgggcgg ggggcgcgcg
     9061 ggcggcgggg cgaagggcgc gctttcgaaa attcgaaagc gccggttttc ggacgggagg
     9121 gcgctcaaac cgctgcggga cgcgggctcg cggccggggc cggattaaga acagatcaat
     9181 tcaatccatt attcgaggcg gccgggaagg caggcgcggg ggccgcggag ggcggcggcg
     9241 ccgggcgaaa gcgccgcgcg ggtcggccgg gggcgttttc gacaattgga aaaccgagtt
     9301 tttccgtggg aagcgcctcc ctcgccgcgc cgggcaaggg aaagcggcgg ggggcgacgc
     9361 ggcggacagc ggcgacggcc gcgcatgttt gcggtggcgc aacgggcgag ggcgtgctat
     9421 cttacggcag accggggcca ccggatcgag gggtgtccgg tctccaacgc cggaggccac
     9481 atgaagtacc acgaagccgc gtactgcggc gatttctgcg gcggctgcga gcagtacctg
     9541 aagacctgct cgggctgcga tccgaagtac aaagaggagt gtccgttcat cgcctgctgc
     9601 cggcagcacc aagcggtgca ctgcggggtg tgcgcggagt ttccgtgcac gagactgtcg
     9661 gcgtttgtgc cggatgaccg gccgggacat ccgcgcgggt accacatcga gaacctgcgg
     9721 tgccgggcga tcgtggggac ggcggactgg ctggcggtgc agcgggcgaa acacgggttg
     9781 cccgcggagg gagtcgcgga ggcggacgag gggggcgggg agtagggggg gcttctgttc
     9841 catcaagagg gcggagcacg cttaaagatg caatctgccc ggggaggagg acgggtgtgc
     9901 cgacatcaaa gcgggaacgc tggtccagag atcagctgtt gattgcgttt aatctttact
     9961 gccgcctgcc gttcgggcgg cttcacagca gacacccgga tgtgatacgc gccgccgaat
    10021 tgatcggtcg gtcccccgac gcgcttgcga tgaaactggt caacttcgca agtcttgatc
    10081 cgcagcatgt agcacgcggg gtccggggac tgaacaacgt cagccgggcc gatcgggata
    10141 tgtggcggga gttttcggga aactgggagg cgcttgcgta tgagagcgag acagcctggc
    10201 gagcgctgct gaccgggcag gatcacgggc gggaaggtga agagtgggaa gcgccggagg
    10261 gagtgaccga gaaggtcatg tccacccggg tacgggtggt gcaggggttt ttccgacgaa
    10321 cggttcttgc ggcttatgag taccgatgcg cctgttgtag attgtccatt cagcagatgc
    10381 tgaatgcaag ccacatcatt ccgtggcggg ttgacaaaca acggcgggcc gacccgacca
    10441 acggcattgc catgtgcgcg tttcacgatc gggcgttcga cagagggttg atcaccctcg
    10501 acggcgatag gcgcatcgtg gtatcgagag aagtgccgac gaagtcgcgg gtcgctatgc
    10561 acagagttgg cttgatcgaa ctagccggcg ctcgggccgc agcgccgtcg agattcgcgc
    10621 cggatgaagc ggcgctggag taccaccgag agaacatttt catcggcgga taggagggaa
    10681 aagatgtacc ggacgttcag gttaacgctt ctggaggctg atgcacggcg ggtgctggac
    10741 gctcttcgga tgcaggagga gttgtgggag cgaaccgctc gatatctgcg agaagactac
    10801 ctcgattcgg acgaaggctg cgtggctgat tgcggggatg agtgggaggc tgcagaaatc
    10861 catcggcact acgccggaat catcacggct ctggaagggc agatcgacga acagcgggcg
    10921 cctagttccc ctcgccgatg attcgcaagc cctcgagcgt gagcgagggg accacctcgg
    10981 tgatgtggcg ggagtggccc tcgatctcga aggccaggcc gccggtcgcg gccaccttgc
    11041 agcggtcgaa gccgcactcc tcgatgatgc ggtcgatgat aaaatcgacc tggccgatca
    11101 ggccgtagaa ggcgccggat ttgagcgcgc cggcggtcga tttccccacg accgtgtcgg
    11161 gcttctcgat gcggacctcg aagaggcggg cggcgcggcg cgccagctcc gccatcgccg
    11221 cctccggacc ggcgatgatg acgccgccga tgtaggcctg ggcggcgtcg aggacgtcga
    11281 aggtggtcgc ggttcccagg tcgacgacga tggcggggcc gccccagcgg gcgagggcgg
    11341 cgacagcgtt ggcgatgcgg tcggcgccga cctggtcggg ggactcgatg cggatcgcga
    11401 tgggcagttt caggcggcag gagacggtga gcggctcgcg gctgaggtag cggcggctgg
    11461 tgcgctcgaa cacgggggtc aggtgcggga cgaccgaggc gatcacgact ttctccaccc
    11521 tggcgggctc gatgcggtgg cgcatgagga aatgggtgat cgacaggccg gcctcatcgg
    11581 gcgtgctgcg gggcggggtg gcgatgcggt agtcgccgac caggtcggcg ccgcggaaga
    11641 ggccgaaaac cacgttggtg ttgccgatgt cgatggcgag cagcatgggg cgaatataac
    11701 ggaaaggccg gcggggggca acggcgaagg agaacggggg gatcatcgcg ggcggaaaac
    11761 ggggccgcgg gagtcggtcg gcgggcgggg gaggggaaac tttttgttgt cgcgcccggc
    11821 gcggcgtctg tataaactat ccctatgacc gccccgaaac tgctgagttg gatcgagctg
    11881 tcgcagagcg cgctgagacg gaacatcgag tcgctgcggc ggctggcggg ggcgcgcatg
    11941 gtggcgccgt cggtcaagag caacgcctac gggcacgggc tgaccgaaat catcaccatg
    12001 cttcgccgca gcggcgggtg cgaatatgtg accgtgcact cactggagga ggcgctcacc
    12061 tgccgccggg cggggtggac ggaggggatc atggtgctcg gcccgctccc gccgggggag
    12121 tacggccagg tgatcgagca ggatctggag ccggcggttt tcgatctggt gacgctgcgg
    12181 gaactcggca agctggggga ccggaagggg gcggcgatcc ggacgcacct caagctcgag
    12241 acggggacga accgccaggg gtttgcagcc caggatctcc ccgccgtggc cgggctgtac
    12301 aaacgttttc gctcgctcgg gcgcccccac ggggcgtcga tgcacttcgc caacatcgaa
    12361 gacaccacgg tgcacgagta cgcggagcag cagttgcgcg cctaccgcga tctgctggcg
    12421 cggttgcggc ggctcgggat cagcccgcgc ctccggcaca cggcctcgtc ggcggcaacc
    12481 atcctctttg agaaaacccg ctacgacctg gtacgaccgg ggatatcggt ctacggccac
    12541 tggccatcga aggaaaccta cctctcctac cgcctccagg ggggcgggaa ccgtctcttc
    12601 gagccggtcc tcacctggaa gacgcggatc acgcagttga agaaggtggc ggcggacagc
    12661 tttgtcggat acggctgcac ctatcggacg acggcgccga cgcggctggc ggtgctgccg
    12721 attgggtact acgacggata tgatcggagc ctgtcgaacc gggcgtacgt gctggtgcac
    12781 gggcggcggg cgccggtgcg ggggcgcatc tgcatgaacc tgatgatggt ggatgtcacg
    12841 gatataagcg gggtgcggct gcacgacgag gtcgtgctga tcggtcgggc gggcgaggag
    12901 cgggtgtcgg ccgaacagat cggagactgg gcggggacga tcaattacga ggttctggcg
    12961 cgcctgtcac tgtctactcc acgtctcata gtggattagg cgggtgagcc gccttcagga
    13021 ccgggggcat tgctgggggt ctttgtgata acgaaaccta ttaccaagtt gtaagataca
    13081 cggttaaatc tgcggtgcgg gccagagaaa agtgttgaca gccgattggg ccgatgctat
    13141 aatctcttgt gattcatttc acatagattg tgaacgactg taacttacag cggcgagtgc
    13201 acagatgaat gtctccagca agaccggtct caagaagaag atttcggaat caacaatcca
    13261 tcggctgtcg ctctactgcc gggcgctgag cctcctggag aaggagaact acgagaccgt
    13321 ttcgtccaag gagctggcga agcgggagaa gctgacgccg gcgcaagtgc gcaaggacct
    13381 gtcgttcttc gggtcgttcg ggacgcgggg gctcgggtat ccggtgaatg agctgaagca
    13441 gcggattgca tcgattctgg ggattgaccg ggtctggcgg gtggccctgg tcggggtggg
    13501 gaatatcggg tcggcgctgg tcagttacaa ggagttcatg aggcagggat tccacatagt
    13561 caaactgttc gacaacgacc agcggaagat cggttcgaac cacaagggaa tcatcgtttc
    13621 ggatatcgcc aatctcgaga aagagctgaa agaggcgggc atcgagatcg tggtgctggc
    13681 ggtgccggcg acggtggcgc agtacattgt ggacgacgtg gtgcgggcgg gggtgcgggc
    13741 ggtcttgaat ttcgccccgg tcaatctgcg ggtgccgccg gatgtctacc tgcgcaacga
    13801 gaacatgtcg atggaactgg agtacctctc gtttgcgctg gtgaacaacc cgccgaagaa
    13861 ggcgagcaag cagggctgat tccggggggc agcggggatg ttcgccggcc acgcagcggt
    13921 tgtcactttc ccccgctctc tgcgccgcac gcggttcgcg cggattcact gatcacaggg
    13981 atcggccggc ccggaactga aaaatcaggc gggaccacca acaataccga tactcatcac
    14041 ccgaagcggg gaggaaaagg aggctacatg aggcgatcgt tacgtcggct gctacggacg
    14101 gccctgccgc cgggaatcgt gctcggcgcc ctggtgtcgg cgacgggcgg gtggagccag
    14161 gagtcggcgg tgtgggggga ccacgcgctg gggacgcgga gctacgatga ctacgcgaca
    14221 ccggactact gcggcacttc ctgccacgtc gacttctatc gccagtggga gcaggcgatg
    14281 atggcgcagg cgtatgtgca ccactgggac gaaatcgagt atttcaagct ggcggtgccg
    14341 cacgcggaga aggatgcgaa ggtggcgggg gtgaaggcgg ggtgcaacgg ctgccatgcg
    14401 ccgctggcgt ttctggcggg cgacgtgccg ccgccccggc cggaggcagg cagccgggcg
    14461 aacgaggcgg tctcctgtga ggtgtgccat tcgattcgcg gttatgccgg ggacacgccc
    14521 tacaacttca actggatcac cgacccgggc gaccgcaaat acggtccgcg gccggccgac
    14581 caggtggcgg agtcgccggc gcacaagatg gcgccgtcgg aattcatccg caccgcggag
    14641 ttctgcggaa cctgccacaa cgagatgagc ccgtatgggg tgtgggtgaa gtcgacccac
    14701 ctggagtggg cggcgggccc gtacgccgcg cagggagtgc gctgccagga ctgccacatg
    14761 acctacgcgc cggggcaggc cgccgcaatg ggcaacgagc actcgaatgt tgcgcagcac
    14821 ctgtttgccg gcgcgcacgt gccggccaag gtgaagggga cgatcgagct caccgtcttc
    14881 ccgagcgtgc gcgaggcggt gatgggcgac ccggtggagt tcaaagtggc gctgttcaac
    14941 cagaagacgg gacacaaatt cccgagcggc tcggtcgagg accggattgt ctggctgcac
    15001 gtggaggcgg tcgatgcggc gggcacggcg taccatcttc cggtcgacga gaaagggttc
    15061 gccggggagg agtacacgat cggggcggac gtgcgcgcct atcaggacat gggcattgcg
    15121 ctcgggcagc cggattttcc gggcgtggcg cgggacgggg tcccggcggg cgaccggatt
    15181 ttccgcatgc cctatttcga tccgcagggg cggatgacga ttcagcagtg gaacaccgcc
    15241 tcgctgggcg tggactaccg gatcggcccg cgcgagacga agatcgagac cttcacgttc
    15301 actgtgccgt acgaggcggc ggagggagag ctgacggtca cggcaactct gtactaccag
    15361 cggctggtga agccggtggc agattttctg gaggttccgg cggaggaggc cgagccgatc
    15421 gtggtcagcg aatactcaac ccacatcacg gtgataccgt aaggagagcg ccatgcgacg
    15481 gctgcttccc gtcctgttaa tcggcgctgc cggggcgctg gcgctggcgg cgctgtttcc
    15541 cgccggcgag gcggcggcga tcccggcgtt cgcccggaag taccggatgt cctgcacgac
    15601 ctgccaccag ccgttcgcgc ggctgaaggc gtacggcgac gacttcgccg gcaacgggtt
    15661 ccggctggcc gacaaagagg cgccggggta tttcgtcgag accggggatc cgaacctgtc
    15721 actcatccgg gattttccgc tctcggcccg cattgagggg tgggcgcggc atgagaccgg
    15781 cacgcggcgc gaaaccgact tctcgacgcc gtacaacgtg aagctgatgt ccgggggggc
    15841 gctcggggac cacctcgcct actatttcta cttcctgctc agcgaacggg gcgaggtcgc
    15901 gggggtggaa gacgcctacc tgatgttcaa cgacctgttc gggcaggatc tcgatctcta
    15961 tgtcgggcag ttccagatct cggatccgct cttcaagcgc gagctgcggt tgacgttcga
    16021 cgattacgcg gtgtacgtcg ccagccccgg cgcttcgggc atcaacctga aatacgaccg
    16081 ggggctaatg gtcacctacg gggcgccgac cggcaccgac gtcgcggtcg agctggtcaa
    16141 cgggagcggg ctggcggaag gcgaggggaa ccggcacgtg tacgaccgcg acaagtacaa
    16201 gaacctgctc gcgcgggtgt cgcagaatct caacgagtac ctgcgcgccg gcatattcgg
    16261 attctacggc aaggaggcgg acgagtacga ccgccgcaac gaggtctgga tggcgggcgg
    16321 ggacgcgacg gtcgggtggg gtccggccga gctcaacctt cagtatgtgg agcggcggga
    16381 cgacaatccc gggttctcgc tgccggcgga tgccaacgag gtcgagacgc gcggcggatt
    16441 cgccgaactg atcgtctggc cgaagggcga ccggtcgagg tggtacgggg cggcgatgtt
    16501 caactatgtg gaatcggggc tgagcatgga gccgttcgcg cagaaaatgt accgggtcgt
    16561 caccgggcac gtcggctacc tcctgcagac gaatctgcgc ctgacactgg agaacagcta
    16621 cgatctggag gccgaggaga actcgctttt ggcgggattc gtgagcgcgt tctagcgggc
    16681 gggggtaagg gagagaaatg agaagccggg cgggtggtcc gcccggcttc gccgcgttga
    16741 agggtcgttg ctgaaccgct tacaggaagt tcccttttag aggaagaagg gcatatttta
    16801 ctccagctcc cgccaggcga cctgaatcat gcggccggtg gtgccgttga taatcaggcc
    16861 gaggctgcgg tcgtagtgga agcgggggtt gttgctgagg tcgatccggt tcgacatgag
    16921 cgagccgtag aagtcggtgt actgggcgag gttgaagttg acgttggggg cgatgacggc
    16981 gcccgtgaat tccgagctct ggcccatcgt gaaacgggtg cccctggagt agacgcggaa
    17041 gttgcccggc ttgccgcccg agttgacggt ggcttt
//
LOCUS       DAOPOE010000071        16942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4041_length_16942_cov_5.433647, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000071 DAOPOE010000000
VERSION     DAOPOE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16942
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4041_length_16942_cov_5.433647"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /gene="infB"
                     /locus_tag="PKM94_09815"
     CDS             complement(<1..1467)
                     /gene="infB"
                     /locus_tag="PKM94_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD66975.1"
                     /translation="MGTTKRVYELAKEYKISSNAMVTVLRELGFEPKSHMSVATVEMI
                     DAIKRKFAAEKQEAKKEMEHRAQVKEAAERKATRIGGIKVNDSKSDVAAWMRRSEKKQ
                     KKKDRRKRKSRHQVDKTAVQKSFRSTMANLSAGKGRRKYRRTPDGRQIEVLEDNVLEV
                     NEFMSVAELAGLMDRKPAELITKLFELGMMATINQRLEMDTITLLAAEFGFEVRQLAE
                     IGEESREEERTANLERRAPVVTIMGHVDHGKTSLLDFIRKTNVAAGEAGLITQHIGAY
                     EVGRDGGRITFLDTPGHEAFTAMRARGSQVTDIIVLVVAADDGVQPQTVEAIDHARAA
                     AVPIIVAINKIDKPSANPDTVKTQLSKLNLMPEDWGGKTIMVEVSAKSGQGVDRLLEM
                     ILLQAEMMDLQADPTIRGQGVIIDSRLERGRGPVVTVLVQKGTCRVGDSIVAGVHSGR
                     VRTIMDDREQLLDEIGPSRPAQITGLDGTPQAGDSLMVV"
     gene            complement(1515..2987)
                     /gene="nusA"
                     /locus_tag="PKM94_09820"
     CDS             complement(1515..2987)
                     /gene="nusA"
                     /locus_tag="PKM94_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOD66976.1"
                     /translation="MSFDMIEAMTLIAREKNIEFDAVLETLEAGLMAAAKKKYAFTDN
                     ITFRFDRKNNELLMIATKRVVEQVADPNVELTLAEAKDIDPEAELGDEVDIYIDYEVE
                     FGRNAIAAAKQILIQKVREAERDRIYDEYIDKVGTLISGVVQQVDKGNVIVNLGRGEG
                     IMPIKEQIPREKFRQGDRIRAFIVDVQKVTRGPQIILSRVSNDFLRKLFELEVPEIYE
                     RVIEIKALAREPGERAKVAVYSSDDRIDPVGACVGIKGVRVQAIVRELNNERIDIVPW
                     SGNPELFVTRALAPAKVVNIDVDDTEQKMTVAVEDDKLSLAIGRSGQNARLASKLTGW
                     KVNIMSETEYNEMKHREAEMLVPVGRLDGVGPKLSERLADHNIGSVQRLANATVEALV
                     KIEGLGEKTAETLIEKAKVFVAELEAEYERKKAMERAAEAAQAAESKAEEKLRPEDVF
                     EEDEDFVTEVDDQAEATAPSMEDLTEDEDENEDESARKDG"
     gene            complement(3001..3456)
                     /gene="rimP"
                     /locus_tag="PKM94_09825"
     CDS             complement(3001..3456)
                     /gene="rimP"
                     /locus_tag="PKM94_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOD66977.1"
                     /translation="MPSVNERFKADLLALVEAPLAAEGAYVADLVVATFKKSATVRVF
                     VYSERGTTLEECARLSRIVGDVLDGTDWFDYGYTLEVSSPGLDRPLVTPRDFRFRTGE
                     TVRVEFRDPARKKLTAELVAATDTDVELRHEGTVERVPLAEIKRAKIVF"
     gene            complement(3553..5271)
                     /locus_tag="PKM94_09830"
     CDS             complement(3553..5271)
                     /locus_tag="PKM94_09830"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOD66978.1"
                     /translation="MRWTHSYIPTLRENQAEAELISHQLLLRGGYIRKLASGIYLYLP
                     LMQRVIHKFSNIVREEMNRAGGLEIAMSVLCPAEVWQESGRYGTIGKEQMRLRDRHDH
                     EQVLCGTHEETVTFLIRGEVKSYRQLPLNLYQIQVKFRDEIRPRFGLMRGREFIMKDA
                     YSFDADEESFARSYQAMVDAYFRIFHRAGLDVVKVESDTGAMGGKAAHEFMLLVDTEA
                     GEEIIMSCDSCDYTANLEKAAFCDPLQPAPEPETKPRETVDTPGAATIEEVTAFLKVP
                     AHRLVKTLLYMADGKPVAALVRGDRELNETKLKNAVGANELVMAAAAEVEQFTGAPVG
                     FAGPIGLKDVPIYADPLVTQMNNFIVGANLFEKHTVNVNLGRDFKAAKIADLTSARHG
                     DGCPKCDGHLVAKHGIEVGNTFMLGTKYSDSLGAKFLDAEGKERPILMGSYGIGVTRT
                     PQAALEKYHDERGICWPKNIAPYQVALIPLNMDKAEHRTAAENIYTALNEANIDVLWD
                     DRTERAGVKFNDADLIGLPVRLVIGDKSLADGKVEMKARRGGEPHLVPIDRIVPAVRD
                     LLDTLP"
     gene            5468..5539
                     /locus_tag="PKM94_09835"
     tRNA            5468..5539
                     /locus_tag="PKM94_09835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5501..5503,aa:Arg,seq:ccg)
     gene            5686..6636
                     /locus_tag="PKM94_09840"
     CDS             5686..6636
                     /locus_tag="PKM94_09840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD66979.1"
                     /translation="MIPSVADLYKIIEQSDLVYVLEHLDSVQVDSIDKARRLNSNMLY
                     LYVDSSSRSLVRHEIDDSLGVIFAIAEGAYEAGDFEAALAAYSEALLLRPSFDPAMVY
                     AGDAYFGMNELDSARAWYQRAVDANFINYQAHWFLGHMMWLQGDSARAIEELTIAHLL
                     NVNHMEIKKVLKSYREDAGGTWRGPDISLHYALTRDTSGVRVRFEAESVAYALVRAVW
                     KYEPGYAESRQGEDWRGSLMQEIAEAAVTWLDFMAGLDDTLAPAWWPQVEELAQEADD
                     SGQVEHFIWYAFIAPELPAAMMLMPREQFDAIREFVDKFY"
     gene            6726..7868
                     /locus_tag="PKM94_09845"
     CDS             6726..7868
                     /locus_tag="PKM94_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD66980.1"
                     /translation="MPKRFLIFVAIVQGILWIGHALLGATAVAFFSIAPGPAAAILFV
                     LSLTFVSASLIGHRRHGPVVRLYYRFAAVWLAVGHVLLLASAAAWAALGLFRLAALGI
                     GPVVGAAAARMAIGSAALGAAAAVAAYGLVKARLIRVARYRVRAAAVPAAWRGRKAVW
                     ISDVHLGYVNGAPYARRVVGLVHRLQPDIVFIGGDLFDGTDGNLDELFTPLRDLTAPL
                     GVYFITGNHEEFFDREAFLAPIRAAANVRILLNEAVEIEGLRLIGVDFRETADPRRYA
                     DVLARLTPGMSPSPAGTEFTVLLRHDPSHNEVAERAGVDVQLSGHTHGGQIWPFTKIA
                     ERVYGARNYGHSQTSRLQTITSSGVGTWGPPMRLGTQSEVVEIVFE"
     gene            complement(8173..9549)
                     /locus_tag="PKM94_09850"
     CDS             complement(8173..9549)
                     /locus_tag="PKM94_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66981.1"
                     /translation="MKTAILPLAAVLLAAALAHGQPVVVLDSLAGDLVCGELAQFIAP
                     YTQYVAIENPDSVQKELRIVLSLHVDPGHQLQDIQVHYLNGFEAVFGFTGTVAWHAEQ
                     QSSTTIVTVAATTNGSGLPGGLPVHDFPVTYLGLSFALGPGEIFEGTESVTVDSAVSP
                     AYGGWYFSSGTASGFAGPLNWPVFETLPNCGSTIVNPPQMPLSASPCYPVAYDFDVAD
                     CFEFPAYLALADAPEGAAIDSISGLFTWQPGVQHVGQQFSVRVIVWEDLCAGTGMDPY
                     YRGPTDEAVFTIVVEDCNPPRFVSGQPNTFVTTSGDDLYISMAVTDPDPLNHYTFDWY
                     VSVQDPVPPCALDPATGVFHYLGTPADTNVYWICLTVSEGIFADTTGFFIYHFDTYIC
                     GDINHFGGVTVGDLTWLINFLFRGGLPPITMLAGDLDCSGDVKVADLTYLVGFLFRGG
                     PAPCASCP"
     gene            complement(9642..10136)
                     /locus_tag="PKM94_09855"
     CDS             complement(9642..10136)
                     /locus_tag="PKM94_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66982.1"
                     /translation="MIRKELWLFLAKLVPLTLLLGYVWFRTVQRQYPDFVDVVADPFF
                     RLVGVRRWWLALVIEHFTNIVPYLALVLASPDLIARWRRTLAALLGGGTALVLGHLLL
                     STAVYHLNEAYGLSKSFYRVIVPLYLVNDALPLILWFAFYPSLPGRLLGIRLFSARDR
                     NTAG"
     gene            complement(10102..10662)
                     /gene="xrtH"
                     /locus_tag="PKM94_09860"
     CDS             complement(10102..10662)
                     /gene="xrtH"
                     /locus_tag="PKM94_09860"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HOD66983.1"
                     /translation="MPERPPASLSDSRPGRSPALRFVLKFVLLLAVLGGAYAWLTARY
                     AESLGWLMDTTAAVTGSVVSLFFGEVHWSGRFVSYRGFPVEVIDECTGLLEMVIFTAA
                     VLAFSTTPGKKLLGLALGLPAIYLFNILRIVVLVIAGASSKPLFDFLHLYFWQATLIL
                     MIASVWIAWIYLVVFRDPKRAVAVPG"
     gene            complement(10759..11076)
                     /locus_tag="PKM94_09865"
     CDS             complement(10759..11076)
                     /locus_tag="PKM94_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66984.1"
                     /translation="MRGDQALSQGVRRCHLTGRCAIAGNSGGSGSAADRPWWILYLSL
                     LVAGLLLIADGFHVAHLHRWTAKLGIALAYSAFVLFVGEGRTLAVVSAGLVCSAVLAT
                     FLW"
     gene            complement(11028..12959)
                     /locus_tag="PKM94_09870"
     CDS             complement(11028..12959)
                     /locus_tag="PKM94_09870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOD66985.1"
                     /translation="MLAWIGIAPAAPQGPAPRIEVIYPKEGQTVAAVDSTFILGNIKP
                     CTHRDQWRLTINGAAVPVHDSGGFIAFLPIAPGPFTFRLEARPVGYDDAGRQHRQKTS
                     FPENEPSAPIACSITVAIPAPAVSYPRDVLRLGREYRPPAGDLYLAAGDRVEAWIQAT
                     PGCRAWFAIPGVADSVPMAETDPRFQPYWGEAVFGEGAVPESLRIAGIYSGFYDVPAA
                     ARADSVRLRYFLAPPGPAAQSHTALESASRAADSALLTLRLPDTVLTDSSTWRLSLNS
                     PVFPFTVRFSDSVKVIRYAPQRAYLAVGPPGGVEALAVGRAGDWYKLRLSRSQVAWVD
                     RRTVTPLPRGILPPQSFLRSLRTYRDSAAVRIELPLAGRHAFRVVEDDRRTLRLQLFG
                     VTADTDWIRYDPRDPLIDYAAWSQPEEGLYELTIRLTKDLWGYDAYYRGSTLYLQVYR
                     APAHVDDLKGKVIVLDPGHSRDPGSVGPTGLSEAEANLAIALELRDRLEGAGARVVMT
                     RIDTAHVELADRPKIAAAAEADLFVSIHNNALPDGVNPLVNNGASAYYYHPHSVNLAR
                     AIHDELVSRTGLADHGFFHGNLAVLRPTGYPAVLVECTFMILPEQEDALKTRAFRVTL
                     ADALCEGIKRFLKEYADAT"
     gene            complement(12982..14010)
                     /locus_tag="PKM94_09875"
     CDS             complement(12982..14010)
                     /locus_tag="PKM94_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOD66986.1"
                     /translation="MMQLDAVKVNLPLKKKFAVSQGEVTVKTNLLTILNNRYSGEAST
                     SIYYGPSIEEMAADLRKGIALLERQTAVTLGTLHDIDRMDIHPAARSALMAAVLNYLS
                     GESRRYPWELVDLGTPIGIKSSMTVGIDATDRMVEAIRSSEYPIIKVKMGHEQDVMIL
                     DALEAGLGKEVRVDANGAWSCAKAEEMIYHLSRKGVRVIEQPTDADYVKEWPHLKGKA
                     DVELIMDEGLNTLDDYMRCRASIDGINIKMDKCGGILHGVALARQAQADRKKVMLGCM
                     VESSVGIAQSVYMSALADYFDLDGPLLLEDDIARGISYHRESINVDREIIGGPKLRRD
                     VLQKYLSE"
     gene            complement(14007..14801)
                     /locus_tag="PKM94_09880"
     CDS             complement(14007..14801)
                     /locus_tag="PKM94_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOD66987.1"
                     /translation="MECAWVTTNILDMRAEPDHRAERVNQLLFADLVSWSAEKRGFAY
                     VRRWDGYRGWADRRFLASLSERRAVAYSRRPAAVVTALQSRLLDFDGRPLPPHLLYYG
                     TIVALDRLEARRLILVAPDGSRLCVKASSVRPIKEYNRGPLTGRDLVREAGRLVGVPY
                     LWGGITAAGLDCSGLVQTVGRRLGLSLPRDSKDQIRTGRPVERARTICGDLVFFPGHV
                     AIAVGRDRIIHSSRGGGGVRIESLAPDAPAYRSDLDQSYLEARRII"
     gene            complement(14891..16063)
                     /locus_tag="PKM94_09885"
     CDS             complement(14891..16063)
                     /locus_tag="PKM94_09885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD66988.1"
                     /translation="MQYTERVRKLESEGAFVVLAKAKALERQGKSIIHLQIGEPDFDT
                     PANVTEAGIKALRAGQTHYAPSGGVMEAREVCASYLARTRHIPVTAENTIIMPGAKPF
                     LFCALIALINEGDEVIVPNPGYPTYRSIVKFVGAKPVPVRLREENDFRFSIDEFRSLI
                     TPRTRMVILNSPGNPTGGVLTWEDLEAIHAEAKKHDLWILSDEIYSRLVYDGEFKSIA
                     MIPGAMERSIIVDGMSKTYAMTGWRLGFAAMPKELAQYFFTLAINNFSSTCTFAQYAM
                     IEALTGPQDDVDKMVAEFRRRRTVIVDGLNAIDGVRCLNPEGAFYVFPNITGTGLTSQ
                     KFADVMLDEAGVACLSGTAFGEYGEGYVRFSYANSVENIREALARIKRVLAGVRTH"
     gene            complement(16078..>16942)
                     /locus_tag="PKM94_09890"
     CDS             complement(16078..>16942)
                     /locus_tag="PKM94_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOD66989.1"
                     /translation="GVAEQNATTIAVGLAKEGKIPVFGTYGVFAAARNLDQIRVSVCY
                     GNYNVLIVGAHGGISVGPDGATHQELEAIFQIAGLPHMHLAIPCDALETEKMTRALLF
                     DVVGPKYLRFAREATPIITRPDSPFRFGEANIFRLRREADRFADAFDITPASEYRSEG
                     EDLTIISCGPEVAEALRAAYILRTDFRLETRVINMHTVKPLDETAIVCAAGETGAVVT
                     AEEHQVGGLGNLVAGVILRRQAETGRPVRFAMVGIADRFGESGQPWQLIKRFGVSAEH
                     IAAKARELVKH"
BASE COUNT         3014 a         5011 c         5840 g         3077 t
ORIGIN      
        1 gacgaccatg agggagtcgc ccgcctgcgg ggtcccgtcg aggccggtaa tctgggccgg
       61 gcgggaaggg ccgatctcgt ccagcagctg ctcgcggtcg tccatgatgg tccgcacgcg
      121 gccggagtgc acgccggcga caatggagtc gcccacccgg caggtgcctt tctgaacgag
      181 gacggtgacg accgggccgc ggccgcgctc gaggcgggaa tcgatgatga cgccctggcc
      241 gcggatggtc gggtcggcct ggagatccat catctccgcc tggagaagga tcatctcgag
      301 cagccggtcg actccctggc cggacttggc cgaaacttcg accatgatgg tcttgccgcc
      361 ccagtcctcg ggcatgagat tgagcttgga caactgggtc ttgaccgtat cggggttggc
      421 cgagggtttg tcgatcttgt tgatggcgac gatgatgggg acggcggcgg cgcgggcgtg
      481 gtcgatcgcc tccacagtct gcggctgcac accgtcgtcg gcggccacga cgaggacgat
      541 gatgtcggtg acctggctgc cccgggcgcg catggcggtg aaggcctcgt ggccgggagt
      601 gtcgaggaag gtgatgcggc cgccgtcgcg ccccacctcg taggcgccga tgtgctgcgt
      661 gatgagcccg gcctcaccgg cggccacgtt ggtcttgcgg atgaagtcca gcagcgaggt
      721 cttgccgtgg tcgacgtggc ccatgatggt gacgacgggg gcgcggcgtt cgaggttggc
      781 cgtgcgctcc tcctcgcgcg attcctcgcc gatttcggcg agctgccgga cctcgaatcc
      841 aaactccgcg gcgagcagcg tgatcgtgtc catttcgaga cgctggttga tggtcgccat
      901 cattcccagc tcgaagagct tggtgatgag ttcggccggt ttgcggtcca tgaggccggc
      961 gagttcggcc accgacatga attcgttgac ttccaggacg ttgtcctcga ggacctcgat
     1021 ctggcggccg tcgggcgtgc ggcggtactt gcgccgcccc ttgccggcgg agaggttggc
     1081 catggtcgaa cggaagcttt tctggacggc ggtcttgtcg acctgatggc ggcttttgcg
     1141 tttgcgccgg tctttcttct tctgcttctt ctcggagcgc cgcatccagg cggcgacgtc
     1201 ggatttcgag tcgttgactt tgatcccgcc gatgcgcgtg gctttgcgtt ccgcggcctc
     1261 cttgacctgg gcgcggtgtt ccatctcctt cttggcttcc tgcttctcgg cggcgaattt
     1321 ccgcttgatc gcgtcgatca tctcgacggt ggccacggac atgtgggatt tcggctcgaa
     1381 cccgagttca cgcagcaccg taaccatggc attggaggag attttatact ccttcgccag
     1441 ctcatagaca cgtttcgtcg ttcccatcag gtgctacctc cccgggcatt gtgggctgcc
     1501 cgatgtgtga gcgatcatcc atccttgcgg gcggactcgt cttcgttctc gtcctcgtcc
     1561 tccgtgaggt cttccatgct gggcgcggtc gcttccgcct gatcgtcaac ctcggtcacg
     1621 aaatcctcgt cctcctcgaa aacgtcctcg ggccggagct tttcctcggc tttggactcg
     1681 gcggcctggg cggcctcggc cgcccgctcc atggcctttt tgcgttcgta ctcggcctcg
     1741 agttccgcga caaacacctt ggccttctca atgagggttt cggcggtctt ttcgccgagc
     1801 ccttcgattt tcacgagcgc ctccacggtg gcgttggcga ggcgctggac ggagccgatg
     1861 ttatggtcgg cgagccgctc ggagagtttc ggcccgacgc cgtcaagacg gccgacgggg
     1921 acgagcattt cggcctcgcg gtgtttcatt tcgttgtatt cggtctcgga catgatgttg
     1981 accttccacc cggtcagctt cgacgcaagc cgggcgttct ggccgctgcg tccgatggcg
     2041 agggataact tgtcgtcttc gaccgccacc gtcatcttct gctcggtgtc gtcgacgtcg
     2101 atgttgacga ccttggcggg ggccagggcg cgggtgacga agagctcggg gttgccggac
     2161 caggggacga tgtcgatgcg ctcgttgttg agttcgcgca cgatcgcctg gacgcgcacg
     2221 cccttgatgc cgacgcaggc accgacgggg tcgatgcggt cgtcggagga gtacacggcc
     2281 accttggcgc gttcgccggg ttcgcgggcg agggctttga tctcgatgac gcgctcgtag
     2341 atctcgggga cttcgagctc gaacagcttg cggaggaagt cgttggagac gcgcgagaga
     2401 atgatctggg gcccgcgggt gactttctga acgtcgacga tgaaggcgcg gatgcggtcc
     2461 ccctggcgga acttctcacg gggaatctgc tccttgatgg gcatgatccc ctccccgcgg
     2521 ccgaggttga cgatgacatt gcccttgtcg acctgctgga cgacgccgga gatgagggtg
     2581 ccgaccttgt cgatgtactc gtcgtagatg cggtcgcgtt cggcctcgcg gaccttctgg
     2641 atgaggatct gcttggcggc ggcgatggcg ttgcggccga actccacctc gtagtcgatg
     2701 tagatgtcga cctcgtcgcc gagctcggct tcgggatcga tgtccttggc ctcggcgaga
     2761 gtgagttcga cgttggggtc ggcaacctgt tcgacgaccc gtttcgttgc gatcatgagc
     2821 agttcgttgt tcttgcggtc gaaacggaag gtgatgttgt cggtgaaggc gtacttcttc
     2881 ttggcggcgg ccatgaggcc ggcctcgagg gtttccagaa ccgcatcaaa ttcgatgttc
     2941 ttttctcggg cgatgagagt catcgcctcg atcatgtcaa acgacataca cgcatctccg
     3001 tcaaaatacg attttcgccc gcttgatctc ggccaggggc acgcgctcga cggtcccttc
     3061 gtgccgcagt tccacatccg tatcggtcgc cgcgaccaat tcggcggtca gcttcttgcg
     3121 cgccgggtcg cggaattcaa cgcggacggt ctcgccggtg cggaaccgga agtcgcgcgg
     3181 ggtcaccagc ggccgatcga ggccgggcga ggacacttcc aaagtataac cgtagtcgaa
     3241 ccagtcggtt ccatcgagca catcgcccac gatccgcgag agccgggcgc attcctcaag
     3301 cgtcgtcccc cgttcggagt agacgaacac gcggacggtg gcactcttct tgaaagtggc
     3361 gacgacgagg tcggccacat acgcgccttc cgcggcaagg ggcgcctcga cgagcgccag
     3421 gaggtcggct ttgaaccgtt cgttcacact cggcatacac cgtttcccgt acaaaaaaat
     3481 gggccactgc ccacccagaa tctgttaaga tatgggaact tgagttgccg cgcaacccaa
     3541 aaatggaacg atttacggca gggtgtccag gaggtcgcgg acagccggca cgatgcggtc
     3601 gataggcacg agatggggct cccccccgcg ccgggccttc atctctacct tcccatcggc
     3661 gagcgactta tcgccaatta cgaggcgtac cggcaggccg atcaggtcgg catcgttgaa
     3721 cttgacccca gcccgctccg tacgatcatc ccaaagtaca tctatattgg cctcgttcaa
     3781 ggcggtatag atgttctccg cggccgtccg gtgctccgcc ttgtccatat tgagcggaat
     3841 gagagcgacc tggtaggggg cgatgttttt gggccagcag atgccccgct cgtcgtggta
     3901 cttttcgagg gcggcctgag gggtgcgggt gaccccgatc ccataggagc ccataaggat
     3961 aggccgctcc ttcccctccg catcgaggaa tttcgcgccc agcgaatcgg agtacttggt
     4021 accgagcatg aaggtgttgc ccacttcgat accgtgcttg gccaccagat ggccgtcgca
     4081 tttcgggcag ccgtcgccgt ggcgagccga cgtaaggtcg gcgattttag cggccttgaa
     4141 atcccgtccg aggttcacgt taactgtatg tttttcaaac agattcgcgc caacgatgaa
     4201 gttgttcatt tgggtgacga gggggtcggc gtagatcgga acgtctttca ggccgatcgg
     4261 gccggcgaac ccgaccgggg cgcccgtgaa ttgctccacc tcggccgccg cggccatgac
     4321 cagctcattg gccccaacgg cgttcttgag cttggtttcg ttcagttccc ggtccccccg
     4381 cacgagcgcg gccaccggct tgccgtcggc catatacagc aaagtcttga ccagtcggtg
     4441 cgcggggact ttcaggaaag cggtgacttc ctcgatcgtg gccgccccag gcgtgtccac
     4501 cgtttccctg ggtttggtct cgggctcggg cgccggttgg agcggatcgc agaacgcggc
     4561 tttctcgagg ttggccgtgt agtcgcagga gtcgcacgac atgatgatct cttcgccggc
     4621 ctcggtgtcg acgagcaaca tgaactcgtg ggcggccttc ccccccatcg cgccggtgtc
     4681 ggactcaacc ttgacgacgt cgaggccggc ccggtggaaa atacgaaaat aggcgtcgac
     4741 catcgcctgg tacgagcgtg cgaacgattc ctcgtcggca tcgaacgagt aggcgtcttt
     4801 catgatgaac tcgcggccgc gcatgaggcc gaaacggggg cggatctcat cgcggaactt
     4861 cacctggatc tggtagaggt tcaggggaag ctggcggtag gatttgacct cgccgcggat
     4921 gaggaatgtg accgtttcct catgggtgcc gcagagaacc tgctcgtggt cgtgccggtc
     4981 gcggaggcgc atctgctctt tgccgatcgt cccgtagcgg cccgattcct gccagacctc
     5041 ggccgggcac aggaccgaca tggcgatttc gaggccgccg gcgcggttca tttcttcgcg
     5101 gacgatgttt gagaacttgt ggatgacgcg ctgcatgagg ggcaggtaga ggtagatgcc
     5161 cgaggcgagt ttgcggatgt agccgccgcg cagcaggagc tggtgggaga tcagttcggc
     5221 ctcggcctgg ttctcgcgga gggtcgggat gtaggagtgc gtccaacgca tggtcgggtc
     5281 ctcatgttcg gtttggtccg ggtcacgtta acgaattggt atattaggca atcaggggtt
     5341 caaagcaact gaaaaactcc ggcctcggcc ggcgccggac gccgagccgt gcgggccggg
     5401 ggcgcgaaaa tggggacaaa atctggttgc gcacaggacg ggaattgggt attaatgggc
     5461 cgtgcatgcc cccgtggtgt aatggataac gcgtcggcct ccggagccgg aaatccaggt
     5521 tcaattcctg gcgggggtat ttctgctgtg tggagaaccg aaagcagtac gggcgtcatg
     5581 aaactttccc ccggcacatt cgcctggctg ccctttgcga cagccgtctc actgacagtc
     5641 gtaccccccg cattatcaca gccgtaccgc cctgatgaga ctcccatgat ccccagcgtt
     5701 gccgacctat ataaaatcat tgagcagtct gacctggtct acgtacttga gcacctcgac
     5761 tccgttcagg tggattcaat cgacaaggcg cgacgcctta actcaaacat gttatacctg
     5821 tacgttgaca gctccagccg gagcttggtc agacatgaaa tcgacgattc gctcggtgtc
     5881 atcttcgcca tagctgaagg ggcgtacgag gccggcgact tcgaggccgc tctggcggct
     5941 tactccgaag cgcttctcct gcggccgagt ttcgatccgg cgatggtcta cgcgggggat
     6001 gcctatttcg gcatgaacga acttgacagc gcacgggcgt ggtaccagcg ggcagtcgat
     6061 gcgaatttca tcaattacca ggcgcactgg ttcctggggc atatgatgtg gctccagggt
     6121 gacagcgccc gcgccatcga agaactcacc atcgcccact tgctgaacgt gaatcacatg
     6181 gagatcaaga aggtgctgaa atcataccgg gaagatgccg ggggaacctg gcgtgggccg
     6241 gatatttccc ttcactatgc gctcacacga gacacctcag gggtacgggt gcgttttgaa
     6301 gcggaatccg tcgcctatgc cttggtccgg gcggtttgga aatatgaacc cgggtacgcg
     6361 gaatcacgtc agggagaaga ctggcgcggt agtctcatgc aggagattgc agaagctgct
     6421 gtcacctggc ttgatttcat ggcgggattg gatgacacac tggccccggc ttggtggcct
     6481 caggttgagg aattggcgca agaggcagac gactcgggcc aggtagagca cttcatctgg
     6541 tacgccttca tagcgccgga actgcccgcg gcgatgatgc tgatgccgcg ggagcaattc
     6601 gatgcgattc gggagttcgt tgacaaattc tactgagcga ttacggaggc gattaggatt
     6661 cgacgagtaa tcaagctgtc ccgccaaaac ctttcccccg gctttccatt atatacctca
     6721 cgtttatgcc caagcgcttc ctcatattcg tcgccatcgt gcagggtatt ctctggatcg
     6781 ggcacgcgct gctcggggcg actgccgtcg cattcttctc gatcgcgccg ggacccgcgg
     6841 cggcgatcct cttcgtcctc tccctcacct tcgtctcggc cagcctgatc gggcaccggc
     6901 ggcatggacc ggtcgtgcgg ctgtactacc gtttcgccgc ggtatggctg gcggtcggac
     6961 acgtgctcct gttggcctcg gcggcggcgt gggccgcgct cggtcttttc cggctggcgg
     7021 cgctcggaat cgggccagtg gtcggagcag cggcggcgcg gatggcgatc ggatcggccg
     7081 cgctgggggc ggctgcggcc gtcgcagcgt acgggctggt caaagcgcgc ctgatccggg
     7141 tggcgcggta ccgcgtgcgg gccgcggccg tgccggccgc ctggcggggg cgaaaggcgg
     7201 tgtggatctc cgacgtgcac ctcggctatg tcaacggcgc cccctacgcc cgccgggtcg
     7261 tcggcctcgt ccatcggctc caaccggaca ttgtgtttat cggcggcgac ctgtttgacg
     7321 ggaccgacgg gaacctcgac gagttattca ctccgctgag agacctgacg gcgccgctgg
     7381 gggtgtattt catcacgggg aaccacgagg agtttttcga ccgggaggcg tttctcgcgc
     7441 cgattcgcgc ggcggcgaac gtgcggattc tgctcaacga ggcggtggag atcgagggcc
     7501 tgcgcctgat cggggtggac ttccgggaaa cggccgatcc gcggcgctat gccgacgtgc
     7561 tggcacggct gacgcccggg atgagcccct cccccgccgg cacggagttc acggtgctgc
     7621 tccggcacga tccctcgcac aacgaggtcg cggagcgggc gggcgtggat gtgcaactgt
     7681 cggggcacac ccacggggga cagatttggc cgttcacgaa aatcgcggag cgggtatacg
     7741 gagcgcggaa ctatggacat tcgcagacga gccgccttca gacgatcacc tcctcgggag
     7801 tgggaacttg gggcccgccg atgcggctgg gaacacagtc ggaggtggtt gaaatagtgt
     7861 ttgagtgaag gggcttgatt ccacgaccga atccgcgaag attccgtttg cgggtcaggt
     7921 cctttatcgt cctgatgggg gtaggcagaa ccgagctgtc aggctgagcg gagtcgaagc
     7981 ctgagacgac cgagacaggt cactcttcga ctccgctcag ggtgacgggg aaaacgtcag
     8041 gaccgcaagg ggcctgacct accgccctgc cgcgtgaacg caggtcaagc accgccgtgg
     8101 gggcttggcg gttgctaaac acaggcgaat cgcgtcaggc acacttctcg ttgcccgctc
     8161 ctctcccctg cgtcacgggc aggaggcgca gggggccgga ccgcctcgga acaggaagcc
     8221 gacgaggtag gtcagatcgg cgaccttgac gtcgccgctg cagtctaggt cgccggccag
     8281 catggtgatc ggcggcagac cgccgcggaa gaggaagttg atgagccacg tgagatcacc
     8341 gacagtgacc ccgccgaagt ggttgatgtc cccgcaaatg taagtgtcga agtgataaat
     8401 gaagaagccg gtcgtgtcgg caaatatccc ctcgctcacg gtcaagcaga tccagtagac
     8461 gttagtgtcc gcaggcgtgc cgagatagtg gaacacgccc gtggccggat cgagcgcgca
     8521 gggcgggacg gggtcctgca cgctgacata ccagtcaaac gtgtaatgat tgagcggatc
     8581 gggatcggtc accgccattg agatgtacag gtcatccccc gaggtggtga cgaaggtgtt
     8641 cggctgcccc gagacgaacc ggggaggatt gcagtcctcg actactatgg tgaacacagc
     8701 ctcgtcggta gggccccggt agtacgggtc cattccggtg ccggcgcaga ggtcctccca
     8761 cacgatgacc cgaacgctga attgctgacc gacatgttga actcccggct gccacgtgaa
     8821 gaggccggag atggaatcga tggcggcgcc ctcgggggca tcggcaagcg ccaggtaggc
     8881 ggggaactca aagcaatcag cgacatcaaa gtcgtacgca acgggatagc agggagaggc
     8941 agagaggggc atttgcggtg gattcacgat cgtgctgccg caattcggca gggtttcgaa
     9001 taccggccag ttcagcgggc cggcaaaacc ggaggccgtt cccgaagaga agtaccagcc
     9061 gccgtaggcg ggcgacacag ccgaatcgac agtaactgat tccgtacctt cgaaaatctc
     9121 ccccggaccg agggcgaagc tgaggccgag atacgtgacc gggaaatcat ggaccgggag
     9181 gccgccgggc agaccggagc cgttggtcgt ggccgccact gtcacgatgg tcgtgctgct
     9241 ctgttgttcc gcatgccatg cgacggtacc agtgaagccg aagacagcct caaatccgtt
     9301 gaggtagtgc acctggatat cctgcagttg gtgaccgggg tcgacatgga gcgacaggac
     9361 gatgcgcaac tccttctgca ccgagtcggg attctcaatg gccacatact gcgtatacgg
     9421 agcaatgaac tgagccagtt ccccacacac gagatccccg gcgagggagt caaggacaac
     9481 cacgggctgt ccatgggcca gggccgcggc aagaagcacc gcggccagcg ggagaatcgc
     9541 tgtcttcata cgacacctgc gtgattcagt gtgagaaatc gttcctgatg cataatatag
     9601 cgatcgtgga taccgacgtc aactagagat accggcagcg atcacccggc ggtgttgcgg
     9661 tcgcgggccg agaagagtcg gatgccgaga aggcggccgg gcagcgacgg gtagaaggcg
     9721 aaccagagga tgagcggaag ggcgtcgttg accagataga gcggcacgat cacgcggtag
     9781 aaggatttcg agagaccgta ggcctcgttg aggtggtaca ccgcagtcga cagcagcagg
     9841 tggccgagga caagggcggt gccgccgccg aggagggccg cgagcgtgcg gcgccagcgg
     9901 gcgatcaggt ccggagacgc caaaacgagc gccaggtacg ggactatgtt ggtgaagtgt
     9961 tcgatcacca gggcgagcca ccagcggcgc acgcccacca gccggaaaaa cgggtcggcg
    10021 accacgtcaa caaagtccgg gtactgccgc tgcaccgtgc ggaaccaaac gtagcccaaa
    10081 agcagagtga gcggaacaag tttagccagg aacagccaca gctcttttcg gatcacgaaa
    10141 caccaccagg tatatccagg cgatccagac ggacgcgatc atgagaatca gggtcgcctg
    10201 ccaaaaatac aggtgcagga agtcgaacaa cggtttcgag gatgcgccgg cgatcaccag
    10261 cacgacgatg cggaggatgt tgaagaggta gattgccgga agcccgagcg ccaggccgag
    10321 gagcttcttc ccaggcgtgg tggagaaggc gaggacggcg gcggtgaaga tgaccatttc
    10381 cagcaggccc gtgcattcgt cgataacctc gaccgggaat ccgcggtagg agacaaagcg
    10441 gccggaccag tgcacctcgc cgaagaagag cgagacgacg ctgccggtga cggcggcagt
    10501 agtgtccatc agccatccga gactctcggc atagcgggcg gtcagccagg cgtaggcgcc
    10561 gccgaggacc gccagcagga ggacgaattt caggacgaag cggagggcgg gggatcggcc
    10621 ggggcggctg tccgacaagg acgccggggg gcgttcgggc aatgacttcc tgcgtgggcg
    10681 tgccatcaac tgcaatttag cccgcggcgg ggaggggcgc aacacccgat tgcgcggccg
    10741 ccaagcccgg gtcgccgctc accagaggaa agtggccaga accgcggagc agaccaatcc
    10801 ggccgagacg accgcgagcg tgcggccttc cccgacgaag aggacaaacg cggagtaggc
    10861 gagtgcgatg ccgagtttgg ccgtccagcg gtgaaggtgg gcgacgtgga aaccgtcggc
    10921 gatcagcagc agcccggcga cgagcaggct gagatagagg atccaccacg ggcggtcggc
    10981 cgccgatccc gatcccccgc tgttacccgc tatggcgcac ctcccggtca ggtggcatcg
    11041 gcgtactcct tgagaaagcg cttgatcccc tcgcacagag catcggccag ggtcacccga
    11101 aacgcgcggg tcttgagggc atcctcctgc tcggggagga tcatgaacgt gcattcgacc
    11161 aggacggccg ggtagccggt cgggcgcagc acggcgaggt tgccgtggaa gaatccgtgg
    11221 tcggccaggc ccgtccgact gaccaattca tcgtgaatgg cgcgcgcgag attgaccgaa
    11281 tggggatggt agtagtaggc ggaggcgccg ttgttgacga gcgggttgac gccgtcggga
    11341 agggcgttgt tgtggatcga cacgaagagg tcggcctcgg cggcggcggc gattttcggc
    11401 cggtcggcca actcgacgtg ggcggtgtcg atccgggtca ttacgacgcg cgcgccggcc
    11461 ccctcgagac ggtcgcgcag ctcgagggcg atcgccaggt tcgcctcggc ctcgctcaga
    11521 ccggtcgggc cgaccgagcc gggatcacgg gaatggccgg gatcgaggac gatgactttc
    11581 cccttcaggt cgtcgacatg cgcgggggcg cggtaaacct gcaggtagag cgtgctcccc
    11641 cggtagtacg catcgtaccc ccagagatcc ttggtcaggc ggatcgtcag ttcgtagagc
    11701 ccctcctccg gctgcgacca ggcggcgtag tcgatgagcg ggtcgcgggg gtcgtagcgg
    11761 atccagtcgg tgtcggcggt gacgccgaag agctggaggc ggagagtgcg gcggtcgtcc
    11821 tcgacgacgc ggaaagcgtg tctcccggcg agcgggagtt cgatgcggac ggcggcggag
    11881 tcgcggtagg tgcggagcga gcgcaggaac gactgcgggg ggaggatgcc gcgcgggagc
    11941 ggcgtcaccg ttcggcggtc cacccaggcg acctgcgagc gggagagccg cagcttgtac
    12001 cagtctcccg cccggccgac cgcgagcgcc tccaccccgc ccggcgggcc gaccgcgagg
    12061 taggcgcgct gcggggcgta gcggatgact ttgacggaat cggaaaagcg aacggtgaac
    12121 gggaagaccg ggctgttgag cgaaagccgc caggtgctgg agtcggtgag gacggtgtcg
    12181 ggcaggcgca gagtgagcag cgcgctgtcg gcggcgcggg aagccgattc caaggccgtg
    12241 tgcgactgcg ccgccggtcc cggcggcgcg aggaagtagc gcaggcggac actgtcggcg
    12301 cgcgccgcgg ccggaacatc atagaagccc gagtagatgc cggcgatccg gagcgactcg
    12361 gggaccgcac cctcgccgaa aaccgcttcc ccccagtagg gctggaagcg cgggtcggtc
    12421 tcggccatcg gcaccgagtc ggcaacgccg ggaatggcga accaggcgcg gcatccaggg
    12481 gtggcctgga tccaggcctc cacgcgatcg cccgccgcca ggtacagatc gcccgccggc
    12541 gggcgatact cccggccgag gcgaaggaca tcgcgggggt agctcacggc cggcgccgga
    12601 atggcgacgg tgatggagca ggcgatcggg gccgacggtt cgttttcggg gaacgacgtc
    12661 ttctggcggt gctggcgccc ggcgtcatcg taaccgaccg ggcgggcctc gagccggaaa
    12721 gtgaacgggc cgggagcgat cggcaggaaa gcgatgaaac cgccgctgtc atgcacgggc
    12781 acggcggcgc cgttgatggt gagccgccac tggtcgcggt gcgtgcacgg cttgatgttg
    12841 ccgaggatga aagtggagtc gacggcggcg accgtctggc cctccttggg atagatcact
    12901 tcgatgcgcg gcgcggggcc ctgcggggcc gcgggggcga tgccgatcca cgccagcacg
    12961 gccgccgacg cgagcagagt cctattccga gaggtatttc tgcaatacat cgcgacgcaa
    13021 tttgggaccg ccgatgattt cgcggtcgac gttgatggac tcgcggtggt aactgatccc
    13081 ccgggcgatg tcgtcctcga gcagcagggg gccgtcaaga tcgaagtagt cggccagggc
    13141 cgacatgtag accgactggg cgatcccgac ggaggactcg accatgcagc cgagcatgac
    13201 tttcttgcgg tccgcctggg cctgccgggc gagggcgaca ccgtggagga tgccgccgca
    13261 cttgtccatc ttgatattga tgccgtcgat agaggcgcga cagcgcatgt aatcatcgag
    13321 ggtattgaga ccctcatcca tgatcagctc gacgtcggcc ttgcccttga ggtggggcca
    13381 ctccttcacg tagtcggcat cggtcggctg ctcgatcacg cggacccctt tgcggctgag
    13441 gtggtaaatc atctcctcgg ccttggcgca cgaccaggcg ccgttggcgt cgacgcggac
    13501 ttctttgccg aggccggcct caagggcgtc gagtatcatc acatcctgtt cgtgccccat
    13561 cttgaccttg atgatcgggt actcggagga gcggatcgct tcgaccatcc ggtcggtggc
    13621 gtcgatgccg acggtcatgg agctcttgat gccgataggt gtgcccaggt cgaccagctc
    13681 ccacgggtag cggcgggatt cgccggagag atagttcagg acggcggcca tgagggccga
    13741 ccgggcagcg gggtggatat ccatgcggtc gatgtcatgg agggtgccga gggtgacggc
    13801 cgtctggcgc tcaagcagtg cgatcccctt tctcagatcg gcggccattt cctcaattga
    13861 gggtccgtag tagatcgagg tcgaggcctc gccgctgtag cggttgttga ggatggtgag
    13921 caggttggtt ttgacggtga cttcgccctg ggaaaccgcg aatttcttct tgagcgggag
    13981 gttgaccttg accgcgtcga gctgcatcat ataattcgcc tcgcttccaa atagctctgg
    14041 tccaaatccg accggtacgc gggggcgtcg ggcgccagcg attcgatgcg cacgccgccg
    14101 ccgccccggg acgaatgaat aatgcggtcg cgtccgacag cgatcgcgac gtgaccggga
    14161 aagaacacca ggtccccgca gatggtacgc gcgcgctcga ccggccggcc ggtgcggatc
    14221 tggtctttgc tgtcgcgcgg caggctcagg ccgaggcgac ggccgaccgt ctgcaccagc
    14281 cccgagcagt cgaggccggc ggccgtgatg ccgccccaga ggtacggcac cccgaccagc
    14341 cggccggctt cgcgcaccaa gtcgcggccg gtcagcgggc cccggttgta ctcctttatc
    14401 ggtcgaacgg aggaagcctt gacgcacaga cggctcccgt cgggggcaac gaggatcaac
    14461 cggcgcgcct cgaggcggtc cagggcgacg atggtgccgt agtacagcag gtgcgggggc
    14521 agcggccggc cgtcgaagtc gagcaggcgg gactggagag cggtgacgac agcggcgggg
    14581 cggcggctgt acgcaacggc ccgccgctcc gacagcgacg ccaggaaacg ccgatcggcc
    14641 catccccggt aaccgtccca gcgccgcacg tacgcaaacc ctcgcttctc ggccgaccag
    14701 gagaccaggt cggcgaacag caactgattg acgcgctcgg cgcggtggtc gggctcggcc
    14761 cgcatgtcca gaatgttggt cgtcacccag gcacattcca tatgaaaacc ttataagtcg
    14821 agcggcagcg ggagacaaca taaaaaagcc gggccatccg gcccggctgg gaaactgcaa
    14881 gggcgccgcc tcagtgcgtc ctgacgccgg ccagcactcg tttgatccgg gcgagcgcct
    14941 cgcggatgtt ctcgaccgaa ttggcatagg agaagcgaac gtacccctcg ccgtactcgc
    15001 cgaaagcggt gcccgaaaga caggccaccc cggcctcgtc gagcatgacg tcggcgaatt
    15061 tctgcgacgt caggccggtg ccggtgatgt tcgggaaaac gtagaaagcg ccttcggggt
    15121 tgagacagcg aacaccgtcg atcgcgttga ggccgtcgac gatgaccgtg cggcggcggc
    15181 ggaattcggc caccatcttg tcgacatcgt cctgcggtcc ggtgagggct tcaatcatag
    15241 cgtactgggc gaaagtgcag gtggaggaga aattgttaat ggccagcgtg aagaaatact
    15301 gggccagttc tttgggcatt gcggcaaatc ccagccgcca gccggtcatg gcgtaggtct
    15361 tggacatccc gtcaacgatg atcgaccgct ccatcgcgcc ggggatcatg gcgatcgatt
    15421 tgaactcccc gtcgtaaacc aggcgcgagt agatctcgtc ggagaggatc cacaggtcgt
    15481 gcttcttcgc ctcggcgtgg atggcttcga gatcctccca ggtgaggacg ccgccggtcg
    15541 ggttgccggg cgaattgagg atgaccatcc gggtgcgggg ggtgatgagg gagcggaatt
    15601 catcgatgga gaagcggaag tcgttctcct cgcgcaggcg gacggggacg ggcttggcgc
    15661 cgacgaattt gacgatggag cggtaggtcg ggtacccggg gttggggacg atgacctcgt
    15721 cgccctcgtt gatcaaggcg atgagggcgc agaagaggaa gggcttggca ccgggcatga
    15781 tgatcgtgtt ttcggcggtg acggggatgt gccgggttcg ggcgaggtag gaagcgcaga
    15841 cctcgcgggc ctccatgacg ccgccggagg gggcgtagtg ggtctggccg gcgcggaggg
    15901 ccttgatgcc ggcctcggtg acgttggcgg gggtgtcgaa gtccggttcg ccgatctgga
    15961 ggtggatgat cgacttgccc tggcgttcga gcgctttggc tttcgccaga acgacgaacg
    16021 cgccctccga ctcgagcttc cgaacccgct ccgtatactg catggcgcaa ctcctctcta
    16081 gtgcttcacg agctctcgtg ccttcgcggc gatgtgttcg gccgagacgc cgaaacgttt
    16141 gatcagttgc cagggctgcc cggactcgcc gaagcggtcg gcgatgccga ccatggcgaa
    16201 acgaaccggg cggccggtct cggcctgccg gcgcaagata acaccggcga ccagatttcc
    16261 caagccccca acttgatgtt cttcggccgt caccacggcg ccggtttccc cggcggcgca
    16321 gacgatcgcg gtctcgtcca agggcttaac ggtatgcatg ttgatgaccc gggtttccag
    16381 gcggaagtcg gtgcggagaa tgtacgccgc ccgcagggcc tcggcgacct cggggccgca
    16441 ggaaatgatc gtgaggtcct ctccctcgct ccggtactcg gacgccgggg tgatgtcgaa
    16501 agcgtcggca aaccggtcgg cttcgcggcg gagccggaaa atgttggcct ccccgaaccg
    16561 aaacgggctg tcgggccggg tgatgatcgg ggtggcctcg cgggcaaacc gcaggtactt
    16621 gggcccgacg acgtcgaaga gcagcgcgcg ggtcatcttt tcggtctcca gggcgtcgca
    16681 cgggatggcc aggtgcatgt ggggcagacc ggcgatctgg aaaatcgcct ccagctcctg
    16741 gtgggtggcg ccgtcgggac ccaccgagat ccccccgtgg gcgccgacga tgaggacgtt
    16801 gtagttcccg tagcacacgg agacgcggat ttggtcgagg ttgcgggcgg cggcgaacac
    16861 gccgtaggtg ccgaagacgg ggattttccc ctccttggcc aggccgaccg cgatggtcgt
    16921 ggcattctgt tcggccacgc cc
//
LOCUS       DAOPOE010000072        16699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4116_length_16699_cov_5.120284, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000072 DAOPOE010000000
VERSION     DAOPOE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16699
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4116_length_16699_cov_5.120284"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKM94_09895"
     CDS             <1..537
                     /locus_tag="PKM94_09895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD66990.1"
                     /translation="GFRPTVFVPTGYLGRPNAWDYSFVFRAEPHLTAGDIRALSACGV
                     AFGSHGHTHTDLTRLSDSTLIEQLRVSRAILEDLTGYPVTALSYPFGRVDSRVSEYAR
                     AAGYCCGYTLRFPSLADTLFTAGRYGVWCFDTPGSVRRKLRPGPLQSFERLKARVTHA
                     LSAGTRLTHRRVRGTARS"
     gene            672..3146
                     /gene="leuS"
                     /locus_tag="PKM94_09900"
     CDS             672..3146
                     /gene="leuS"
                     /locus_tag="PKM94_09900"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOD66991.1"
                     /translation="MRFPYREIEAKWQQRWIERGVHRAPRMPDPARKWYQLEMFAYPS
                     GDIHMGHFRNYVIGDAVARQRLMLGADLLHPFGWDAFGLPAERAAIQRGVPPAEWTRS
                     NVGVSRATLQRTGVSFDWTREIDSSRPDYYRWTQWMFIQLFRHNLAYRKRGFVNWCPE
                     DKTVLANEQVKEGRCERCGTEVIKKDQIQWYFKITAYADRLIDDLDLLPGWPENVKTM
                     QREWIGRSHGAEIDFTIEETGEKLPIFTTRPDTIFGVTFMAISPESDLLQSLKLEPPY
                     SEAVAGYQRKAFARTDIERAAATTEKDGVFTGKYAVNPFSGERVQLWVADYVLAGYGT
                     GAVMAVPAHDTRDFAFARKYGIPIKVVIHPDANTSLNANEMADAFTDYGPMVNSGRFN
                     GLAGEEALSKVTQFAEQQGFGRGKVNYKLKDWSISRQRYWGCPIPIIHCESCGAVPVP
                     ENQLPVLLPPVQDYLPKGRSPLADVSSYMNVACPKCGRAAQRDPDTMDTFVCSSWYYL
                     RYLDPDNESAPFDKELARRWLPIDLYIGGITHATGHLIYFRFFHKFLQDIGWVFCPEP
                     AARLFNHGMVQDAHGVTMSKSKGNVVSPVAVMEEHGTDIARLAMFFTAPSEKEVLWSE
                     DSVTGVEKFALNKIWPLAGYVRGARPDLKRYFKSAELNEFEWALYIKLNQTVKRVTED
                     FERLQFNTSIAALMELLRDYDAARIVSNEFNDYLILKSIQLAAPLIPHMAEELWEMAG
                     HDDSIFRSAWPVYDPEAVVGETIEIAVQINGKLRDTIRVARDADQKTVEAAALAGEKV
                     AKFTEGKTIIKVIYVPGRILNIVVKG"
     gene            3384..4073
                     /locus_tag="PKM94_09905"
     CDS             3384..4073
                     /locus_tag="PKM94_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD66992.1"
                     /translation="MKLSIIIPVYNEVATLPDIIDHVRNAPVADKEIIVVDDYSTDGT
                     RDLLKTRLEPLVDLVIYQPRNFGKGYALKTGFGRATGDIVIVQDADLEYDPNEYPALI
                     EPIVSDKADVVYGSRFMGGGPHRVVYFWHYVGNRFLTLLSNMFTNVNLTDMETCYKVF
                     RRDLIQSIDIQENRFGFEPEITAKIARRRVRIYEIGISYYGRTYDEGKKIGWRDGLHA
                     VWCILKYNLGR"
     gene            complement(4608..5582)
                     /locus_tag="PKM94_09910"
     CDS             complement(4608..5582)
                     /locus_tag="PKM94_09910"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD66993.1"
                     /translation="MGQTILVTGSSGTVGTALVRALEQAGHKVIPLDLRRSYWDARID
                     RRTVFHDLRRPLTGLRLRRKPDTIIHLAANARVHELVLHPEQARDNYLMTFNVLEYAR
                     RAGITRVCYASSREVYGESRSGERRKEESTHVCAIKSPYTASKYGGEALMHAYGECYG
                     IQPVVVRLSNVYGRYDVSERVIPLFIYYARRNREITVFGAEKKLDFTYIDDCVGGFLR
                     IVDRFERSAGMTFNLSAGRGERILDLARWIVEMLGSQSTVRVSEKRVGEISSFTGDIR
                     LARMYLGYEPRVSLREGLPPTIAWYLQAIREPRVYAAQRRALARLGWL"
     gene            complement(5587..6888)
                     /locus_tag="PKM94_09915"
     CDS             complement(5587..6888)
                     /locus_tag="PKM94_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD66994.1"
                     /translation="MTPRPNILVVGAGYVGLATAVFLADKGFPTTVVEKNSALVETLR
                     RGRLHFRERLLAERLREVIGERTLMVAEPEKALYQRADLIFIAIDSANRKTWQMRTAT
                     FAEIAAWIGEVRRKTAPTVVLKSTNVLGFAGEFRRLLDAAPFGKAVKLVVNPEFLREG
                     NALEDTARPFRIVIGSREKRDAARLAMLYRQVYRTGVPIVHTDWTSAELIKLASNVYL
                     AYRLAFIHEIADFARREGLDLEAIRMGIGLDQRIGLDYFTPGLGFGGSCLPKDCLLIN
                     SRETGNGFTFQSAQTAMAVNEGVLENLVKMLAEKLGGLAGKKIALLGAAFKPDVDDTR
                     DSHAVRLAELLKAKRAKVAIYEPYLAGRDRIPETEFALEKDVAAALDGAAAVIIGTAH
                     KRLAMLRPREVAALVANKLVIDYFRVLNRGGWREAGFEVIG"
     gene            complement(6957..7871)
                     /locus_tag="PKM94_09920"
     CDS             complement(6957..7871)
                     /locus_tag="PKM94_09920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOD66995.1"
                     /translation="MIVKRTTLPSVLIMLAAFVFFSANTVYFLEAEDGPGLSPLQRNY
                     QFDFSTVREGPRLNTKAAILVDYENGRVLYAKEADEVRPIASISKLVTAMVVLDKGID
                     MSRTETITQEDAKESSRSRLRVGYELTIRDLLHAALMNSDNRAARALARVTSGSYGDF
                     AKAMNKKARKLGLRNTYFVEPTGLSEQNVSTAHEVAKILHYAYDYPLIREISSKESYR
                     VRVLNRKHASVSMSNTNGLMHSQFRVLSGKTGYIRESDYCLTTMVRDRTGRRLTAVVL
                     GVPGDKLRFRECRRLIEWGYKSCADLAT"
     gene            complement(7903..9213)
                     /locus_tag="PKM94_09925"
     CDS             complement(7903..9213)
                     /locus_tag="PKM94_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD66996.1"
                     /translation="MRKFWVVFKREYAQVVKKKSFLIGVLLTPALMAGFVVLPMLLAD
                     PGLDRPQSIAIIDQSSAGFGERLQERLVEVKIKETEAPLYDFRGFFTVAPGDDPGYRR
                     VLDSLTAAINAQELKYVVVIRPNPQLSDTGVFAVTNATNFTILQTIENHASQLLSMER
                     LRQSHVNVSVDSVLALTRRLDLQVRDAKGETMPFEYKYFGALIFVMVMFGMILGYGQM
                     VMRSVIDEKNSRIMEVLISSVTPFQLMLGKVLGLGAATFTQAAIWVALGAVLYANKAA
                     LSVDSSIDRLLFNPVIVVFFVLYLTTGYLLFSTLFALVGSIVNSEKEAQNFVFPITMS
                     LVLPVMIGIHVVQEPNSTLSLVLSYIPFLTPTMMMMRIIFIAPTMTEYSLFGGIVGQA
                     VLGFVLVSLTTVAVIWLTAKIFRVGILMYGKRPTLPEIVKWVRY"
     gene            complement(9206..10138)
                     /locus_tag="PKM94_09930"
     CDS             complement(9206..10138)
                     /locus_tag="PKM94_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD66997.1"
                     /translation="MQLTLDHIRKEFDGKVAVNDLSLEVPEGVIYGIIGPNGAGKTTT
                     IRMVMNITVPDRGRVLVDGRTAGPDFNNRVGYLPEERGLYKKMTLEEVILYMAELKGV
                     GRREMLPKIGPWLERMNLAAYRHRKVEELSKGMAQKLQFVTTIIHEPEIVILDELFSG
                     LDPINIELIKDILLEIKRSGRTILFSTHVMEQAEKLCDHVCMIAGGEKVLDGPLGRVK
                     AQFGENTIQLEIEGDALFVGELPGVRNVTQFTNYIEIDLGDGARPNEILRAVTDRVPV
                     RRFQIIEPSLYSIFIRMARPESDAAAAGGEAAHA"
     gene            complement(10219..10992)
                     /locus_tag="PKM94_09935"
     CDS             complement(10219..10992)
                     /locus_tag="PKM94_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOD66998.1"
                     /translation="MEIRIGTSGFSFEDWRGSFYPETIDKGKMLDYYVERFSTVEINS
                     TYYRIPHPAVMRNLAKKAPEGFDFVVKLPQSVTHRRQDIEEDLRQFLQAVEPMEASGK
                     LSGLLAQFPYSFRFSPDSLDYLGALRAALAPRPLFTEFRHRSWVNRTMYDRLRAEGIG
                     YVCVDEPELPNLIKPDLFTTGPTAYLRLHGRNAEYWWEGGALRYDYRYSREQLEEWKQ
                     KIGKLAAKGEAAKLYVYFNNCHLGQAAANAVEMRELLGL"
     gene            complement(11241..11855)
                     /locus_tag="PKM94_09940"
     CDS             complement(11241..11855)
                     /locus_tag="PKM94_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD66999.1"
                     /translation="MDWGTAVAGALTLAILSFLYRDNPVYRMAESLLIGVAIGYFLVI
                     TWTNTLMDLLVRPLFGDGRLGLIAPLVFGLLMFGRFHPSTKSWSRLSMAVLIGSGAGV
                     AIPVMLEARTLKQMSATVMPLVGPTGMPNWSAIVVMVGVVTTLSYFYFSREHKGALGR
                     SAKIGVYFLMIFFGTTFGYTVMSRMSTFIGRVEFLLSDFLRLTS"
     gene            complement(11855..12670)
                     /locus_tag="PKM94_09945"
     CDS             complement(11855..12670)
                     /locus_tag="PKM94_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67000.1"
                     /translation="MKLESRHWIFLGLGAAVVAAMALQVNLQVPASDDTLGMHRFIEE
                     VPAGSTIIVSFDHEASSLPEIRPLALAVLRHAFSRNLKIVGTALLAEGTGVGYRLVQQ
                     TAREYDKRYGEDWVYLGFKPQYIAAILSMGESFAKTWPEDYLGRPYTDLPMLAEIETY
                     GQIAGVVSVADGSLTTHWIEYAASRYRVRVAAAVTAAMVTTYDPYLASGQLQAMVGGL
                     RGAAEYEQLIARGGGGERGMLAQTVSHLYVVALIVVGNLVFFATRRREKGGGG"
     gene            complement(12667..13293)
                     /locus_tag="PKM94_09950"
     CDS             complement(12667..13293)
                     /locus_tag="PKM94_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67001.1"
                     /translation="MGRRLPQFIVLVFGAVMFLQYFSDAPAARSINSAVLVDYWQIIF
                     AFTLLVGVVSYVRTATAGIGRAADWPYRAVSLAGVALMPVLAVLWGIRAESPFMWVFE
                     NVQVPMQATVFALLAFFVASASYRGFRARSAPAAVLLAAAFLTILTRSALGDWYPDWV
                     NRVAEWVRDVPSMAARRAILIGIGLGSLTTSLRVILGIERTWLGRGGR"
     gene            complement(13294..14100)
                     /locus_tag="PKM94_09955"
     CDS             complement(13294..14100)
                     /locus_tag="PKM94_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67002.1"
                     /translation="MAAVPLIAAAADPPWFDASTSVTLVAAAVAIVIVLYTTYHRKSR
                     ARYRMREIAGLRAIEEAVGRATEMARPILYTPGWGGDIQRPTTIASLNILSHVAEKAA
                     QYDCRLVFPTHDPVIMAVAQEVMRESCARAGFPDRFREDSASYVSSSQFGYAAAVEGQ
                     ISRLKPASVFLLGTFEAESLILAETANINKSIQIAGTDSTIQLAFFIVACDYVLIGEE
                     LFAASGYLSEDPSIIASVRAQDILKVLIVVLLIAAVVWATFAPDSTWWRL"
     gene            14229..15440
                     /locus_tag="PKM94_09960"
     CDS             14229..15440
                     /locus_tag="PKM94_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOD67003.1"
                     /translation="MRIRATILAAALLLAGPARAATVEWWQFWTDPAIKPVIEQMVAE
                     FEQANPDIKVRLTDLTWANGHEKIVLAFSSGTAPDVVELGSDWIAQFAANGRLADLSG
                     LVAGDTAGYDGWSMAADRGGLYAWPWILGTRVLFGNRDLITRAGYDTAFYPVNWPQLT
                     EAAKKVRALGPNFYGWGSNTAEKHRLYKKYLPFFWSAGAQLFSDDGRMCVVASSRAVS
                     ALRTYQQLHDSAGYVDTQRGIEDAFLDGRIGFIISGDWLLKRIELENRRINLISTLIP
                     GPKFPGRSFLGGEFLAVSADSDVKEAALRLIRFVTSPVNQVRFCRANRSANPSSREAQ
                     RDPYFASNPHLQTFIAALKSAQHPPVDPDWVYIEDILEKAVEEALFGSHLVAVPLLEA
                     RYKIEELKGLK"
     gene            15437..16306
                     /locus_tag="PKM94_09965"
     CDS             15437..16306
                     /locus_tag="PKM94_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOD67004.1"
                     /translation="MRRSRLTALLLLSPWILSFAVFWLFPLVYSLVMGFTDYRLLAPA
                     WRWIGLANYRSLLADESFLAAVENTFIFVLGTVPVTTVIALGLALLVNRQFPGRGLFR
                     AGYFIPSITSMVVVALIFTNLYQRGGYIAALAAMVGLPAPEHGFLFSDKTALASIMAM
                     DVWMAVGYYMLIFLAGLKSIPDELYEAARIQGAGRARQFFSITLPLLRPVALFIVVIN
                     SIKSFQVFVEIFVMTKGKFDTASMVYFIYDAGLTTAFRFGYASAAAYLLFLIIAVFSV
                     VQFALLRRRQPLW"
     gene            16300..>16699
                     /locus_tag="PKM94_09970"
     CDS             16300..>16699
                     /locus_tag="PKM94_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67005.1"
                     /translation="MVARRAASAVRYAVLGVILAVMVFPLVWMFRVAVMSPGAGVGLA
                     DLFTSDVTFRNFADLFASGRIGRYLFNSAFVALVVTAGNILFCFMAAYVLARRPGLWT
                     RVLFASVVVVLMIPAHILIVPLYVLMLKARL"
BASE COUNT         3004 a         5663 c         4980 g         3052 t
ORIGIN      
        1 ggctttcgcc cgaccgtttt tgtccccacc ggataccttg gccgccccaa cgcctgggac
       61 tatagctttg tcttccgcgc cgaaccgcac ctgacggccg gggacattcg cgctctctcg
      121 gcgtgcggtg tcgcttttgg ctcccacggc cacacccaca ccgatctgac ccgcctttcg
      181 gattcaactc tgatcgaaca gctccgcgtg tcccgcgcaa ttctggagga tctcaccggc
      241 tatccggtga ccgctctcag ctaccccttc ggacgggtcg attcccgggt gtccgagtac
      301 gcgcgcgccg ccggctattg ctgcggctac acgctgcgct ttccctcgtt ggccgacacg
      361 ctgttcacgg ccggccgtta cggcgtgtgg tgtttcgata ccccgggttc ggtccggcgc
      421 aaactcagac ccggtcccct gcaatcgttc gagcgtctca aagcccgggt cacccacgcg
      481 ctctcggccg ggacccgcct cacccatcgg cgcgtcaggg gaaccgctcg ttcgtagcgg
      541 cgccgcgggt cggccgcctc tgcacgcggc gctcaaacta acccttgcct cctccgccgc
      601 attgtgctac tttcgtccct actctggagt gaccgaggat gttgtcgatg gggacccgta
      661 agactgaaac catgcgcttt ccgtacagag agatcgaggc caagtggcag cagcggtgga
      721 tcgagcgggg tgtgcaccgc gccccccgca tgcccgatcc cgcccgcaag tggtaccagc
      781 tcgagatgtt cgcctatccc tcgggcgaca tccacatggg ccatttccgc aactatgtca
      841 tcggcgatgc cgtggcgcgc cagcgcctca tgctcggcgc cgacctcctg cacccgtttg
      901 gctgggatgc tttcggcctg ccggccgagc gcgccgccat ccagcgcggc gtcccgcccg
      961 ccgagtggac ccgaagcaac gtcggcgtgt cccgcgccac gctccagcga accggcgtct
     1021 cattcgactg gacccgcgag atcgattcct cccgccccga ctactaccgc tggacgcagt
     1081 ggatgttcat tcagctcttc cggcataacc tcgcctaccg caagcgcggc ttcgtcaact
     1141 ggtgcccgga agataagact gtcctggcca acgagcaggt caaggaaggc cgttgtgagc
     1201 gctgcggcac cgaggtgatc aagaaagacc agatccagtg gtacttcaaa atcaccgcct
     1261 atgccgaccg cctcatcgat gacctcgatc tgcttcccgg ctggccggaa aacgtgaaaa
     1321 ccatgcaacg cgagtggatc ggccgctccc atggcgccga gattgatttc acgatcgagg
     1381 aaacggggga gaagctcccg attttcacca cccgccccga cacgattttc ggcgtcactt
     1441 tcatggctat ctcgcccgaa tccgacctcc tccagtcgtt gaaactggag ccgccctact
     1501 ccgaggcggt ggccgggtac cagcgcaaag cattcgcccg gaccgacatt gaacgggccg
     1561 ccgccaccac ggaaaaagac ggtgttttca ccggcaagta tgcggtcaac ccgttcagcg
     1621 gcgagcgtgt ccagctctgg gtcgccgatt acgtcctcgc cggctacgga acgggcgcgg
     1681 tgatggcggt gcctgcccat gacacgcgcg attttgcctt tgcccggaaa tacggcattc
     1741 ccattaaagt cgtgattcat ccggatgcga atacttcgct caatgccaat gagatggccg
     1801 atgccttcac cgattacggc cccatggtga actccggtcg attcaacggt ctggcggggg
     1861 aagaggccct ttccaaagtc acgcaatttg ccgaacagca ggggtttggc cgaggcaaag
     1921 taaactataa gttgaaagac tggtcgatct cccgccagcg ttactgggga tgcccgatcc
     1981 caattattca ctgcgagagc tgcggcgctg ttcctgttcc cgaaaaccaa ctcccggtcc
     2041 tcctgccgcc ggttcaggat tatctcccga aaggccgttc gcctcttgcc gacgtttcct
     2101 cctatatgaa tgtggcctgc cccaagtgcg gccgcgccgc ccagcgcgac cccgacacca
     2161 tggacacttt cgtctgctcc tcctggtatt acctgcgcta cctcgatccg gacaacgagt
     2221 cggctccttt cgacaaggaa cttgcccgcc gctggctgcc gatcgatctc tatatcggcg
     2281 gcatcaccca cgccaccggc cacctgatct acttccgatt cttccacaaa ttcctccagg
     2341 acatcggctg ggtcttctgc ccggaacccg cggcccggct gttcaaccac ggtatggtcc
     2401 aggacgcgca tggtgttacg atgtcgaaat ccaagggcaa cgtcgtctcg cccgttgccg
     2461 tcatggagga acacggcacc gacatcgccc ggttggcgat gttcttcacc gcgccgtcgg
     2521 agaaagaagt cctgtggtcg gaggattcgg ttaccggggt tgagaaattc gccctgaaca
     2581 agatttggcc gctggccggt tatgtgcggg gcgcgcggcc cgacttaaag cgttacttta
     2641 agtccgccga actgaacgaa ttcgaatggg ccctctatat caagctcaac cagaccgtca
     2701 agcgcgtcac cgaggatttc gaacgcctcc agttcaacac ctcgatcgcc gccctcatgg
     2761 agcttttgcg agactatgac gcggcccgga tcgtcagcaa cgagttcaac gactacctga
     2821 ttctcaaatc gatccagctc gccgctccgc tgatcccgca catggccgag gagctctggg
     2881 agatggccgg gcatgacgat tcgatcttcc gctcggcctg gccggtctac gacccggagg
     2941 ccgtcgtcgg cgagacaatt gaaatcgcag tccagatcaa cggcaaattg cgcgacacca
     3001 tccgggtcgc ccgcgatgcc gatcagaaga cggtcgaggc agcggcgttg gccggcgaaa
     3061 aggtcgccaa attcaccgag ggcaaaacga tcatcaaagt catctacgtc ccaggccgaa
     3121 tcctcaatat agtggtgaag ggctgatcgt gtgccgccgg acgggtaccg aaatcctgga
     3181 tggttcgagc agtccggaag ggtcagaagg ccaggataga tcgaaaacct ggctccgagg
     3241 tccggacggg accggtaaac tcgacaggga caccgctacg ccgatagaac gagcgacccg
     3301 cttggccgag cagtggggcg ggttctgacg tgcggcggtt aacataatat gtcttatagg
     3361 acactaaact cccgctggcc atcatgaaac tctccattat aatccctgtc tacaacgagg
     3421 tcgccaccct ccccgacatc atcgaccacg tccggaacgc cccggtcgcc gacaaggaga
     3481 ttatcgtcgt cgatgactac tccacggatg gtacccgcga cctgctgaaa acccgccttg
     3541 agccgctggt cgacctggtc atttaccagc cgcgcaattt cggcaagggt tacgcgctca
     3601 agaccggttt cggccgcgcc accggcgaca tcgtgattgt ccaggacgcc gacctggagt
     3661 acgatcccaa cgagtacccc gccctgatcg agccgatcgt ctccgacaag gccgacgtcg
     3721 tctatggctc ccggttcatg ggcggcggcc cgcaccgcgt cgtttatttc tggcactatg
     3781 tcggcaaccg ctttttgacg ctgctttcga acatgttcac gaatgtcaac ctgaccgaca
     3841 tggagacctg ctacaaggtt ttccgccgcg acctgatcca gtcgatcgac attcaggaga
     3901 atcgcttcgg gtttgagccg gagatcactg cgaaaatcgc ccgccgtcgt gtccgcattt
     3961 acgaaatcgg aatatcgtat tatggccgta cgtacgacga gggcaagaag atcggctggc
     4021 gcgacggcct gcacgcggtg tggtgtatat tgaagtacaa cttggggcgg taggcgcaat
     4081 cacggatgat tggaccggtg gcccgccctt tgcggctttc aaagggtaac ccacagctcc
     4141 gctgtgggga cttaacgtta ttcaatcagt gcgctgcgca acctgtccag cggcctgggc
     4201 gcgtcctgcc gtcccaagta ggaattacag ctcgaccagt tcccgtcacc aaggcaagta
     4261 cccacggcag agctgtgggc gaccggcgct ctcgctcggg aggtcagccc atgaccccca
     4321 gaataggcgt cccccgcaac ccgatcgggg aattgccgcc ccccggtact gtcattcccg
     4381 cggaggcggg aatcccggca acgcgcctct cccctgccgc tgccccagat tgccaagagc
     4441 aaagcctcca agactggacg aaaccacccg ccgaagagag gcagtctgag agagaggcag
     4501 acgaaacaga gccgatggac gaaatctcaa aaacgagcga agcaggccca gtcgatgatt
     4561 acgggtgaaa agaaatcaga caaacgcgcc caccccccgc cgcagcgcta cagccacccc
     4621 aaccgggcga gcgcccgccg ctgcgccgca tacactcgcg gctcccggat cgcctgtaaa
     4681 taccacgcga tcgtcggcgg cagcccctcc cgcaagctca ccctcggctc ataccccagg
     4741 tacatccgcg ccagccggat atccccggta aacgacgaaa tctccccgac ccgcttctcg
     4801 ctcacgcgca ccgtcgattg cgaacccagc atctccacga tccacctggc cagatccagg
     4861 atcctctccc cccgcccggc cgacaagttg aacgtcatcc ccgcgcttcg ctcaaaccgg
     4921 tccacgatcc ggaggaatcc cccgacgcag tcgtcgatat acgtgaaatc aagcttcttc
     4981 tccgcgccga acaccgtgat ttcgcgattg cgccgcgcgt agtagatgaa caggggaatc
     5041 acgcgctcgg agacgtcgta gcgcccgtag acgttggaca gccgcacgac caccggctgg
     5101 attccgtagc actcgccgta ggcgtgcatg agggcctcgc cgccgtattt cgacgccgtg
     5161 tagggcgact tgatcgcaca cacgtgcgtc gactcctcct tgcgccgctc gccggaccgc
     5221 gactcgccgt agacctcgcg cgacgaggcg tagcagacgc gcgtgatccc cgcccggcgg
     5281 gcgtactcga gcacgttgaa cgtcatcagg taattgtcgc gcgcctgctc cggatggagc
     5341 accagctcgt gcacccgggc gttggccgcc aggtgaataa tcgtgtccgg tttccgccgc
     5401 agccgcagcc ccgtcagcgg ccggcgcagg tcatggaaca ccgtccgccg gtcgatgcgg
     5461 gcgtcccaat aggagcggcg aagatcgagg gggatgactt tgtgccctgc ctgctccagc
     5521 gcccgcacca gcgccgtccc caccgtcccc gatgaaccgg tcacgagaat ggtctgcccc
     5581 acgcctctac cctatcacct cgaaaccggc ctcgcgccac ccgccgcggt tgagcacgcg
     5641 aaaatagtcg atcacgagct tgttggccac cagggcggcc acttccctgg gccgcaacat
     5701 cgccagccgc ttgtgggcgg tcccgatgat caccgccgcc gccccgtcga gagccgccgc
     5761 gacgtctttc tccagggcga actcggtctc gggaatcctg tcccgccccg cgaggtaggg
     5821 ttcgtagatc gccactttgg cgcgcttggc cttcagcagc tcggccagcc gcacggcgtg
     5881 cgagtcgcgc gtgtcgtcca catcgggctt gaacgccgcg ccgagcagtg caatcttctt
     5941 ccccgccagc ccgccgagtt tctcggcaag catcttcacg aggttctcca gcaccccctc
     6001 gttcaccgcc atcgccgtct gggccgactg gaaggtaaag ccgttgccgg tctcgcgcga
     6061 gttgatgagc aggcagtcct tgggaaggca ggatcctccg aatcccagcc cgggcgtgaa
     6121 gtagtccagg ccgatccgct ggtccagccc gatccccatc cggatcgcct ccagatcgag
     6181 tccctcacgg cgggcgaaat cggcgatctc atgaatgaac gcgagccggt aggcgaggta
     6241 gacgttggag gcgagcttga tcagttcggc cgacgtccag tcggtgtgca cgatcggcac
     6301 gcccgtgcgg tagacctgcc ggtagagcat ggccagccgc gccgcgtccc gcttctcgcg
     6361 cgacccgatc acgatccgga acgggcgggc cgtgtcctcc agggcgtttc cctcgcgcaa
     6421 gaactccggg ttgaccacca gcttgaccgc tttcccaaag ggggcggcgt cgagcaggcg
     6481 gcggaactcc ccggcaaacc ccagaacgtt ggtcgacttg agaacgaccg tcggtgccgt
     6541 cttgcgccgg acctctccga tccaggcggc gatctcggcg aaggtggcgg tgcgcatctg
     6601 ccacgttttg cggttggcgg agtcgatggc gatgaagatg aggtcggccc gctggtagag
     6661 cgctttctcc ggctccgcca ccatgagggt gcgttccccg atcacctccc gcagccgctc
     6721 ggcgagcaga cgttcgcgga aatggagccg cccccggcgg agcgtctcca ccagggcgct
     6781 gttcttctcg accaccgtgg tcgggaaacc cttgtcggcg aggaatacgg cggtcgccag
     6841 accgacatac ccggcgccaa cgaccagtat gttgggacga ggggtcatac gccggcgcca
     6901 gtataacctc ttaaccgaaa aatggcaacg gttttagccg gattcgccgg ggcccatcag
     6961 gtggccagat cggcgcagga tttgtacccc cactcgatca ggcgccggca ttcccggaag
     7021 cggagcttgt cccccggcac gccgagcacc accgccgtga gacggcggcc ggtccggtcg
     7081 cgcaccatgg tcgtcagaca gtagtccgat tccctgatat atcccgtttt ccccgagagc
     7141 acgcggaact gcgagtgcat gaggccgttg gtgttggaca tgctcacgct cgcgtgcttg
     7201 cggttcagaa cccgcacccg gtaggactcc ttgctggaaa tctcccttat gagcggatag
     7261 tcgtaggcgt agtggagaat cttggcgacc tcgtgcgcgg tcgaaacatt ctgttccgag
     7321 agaccggtcg gctcgacgaa gtaggtgttc cgcagcccga gcttgcgcgc tttcttgttc
     7381 atcgccttgg cgaaatcgcc gtacgatccg gaggtgacgc gggcgagcgc ccgcgcggcg
     7441 cggttgtccg agttcatcag cgccgcgtgg agcagatccc ggatggtcag ttcgtacccg
     7501 acgcggaggc gcgagcgcga cgattccttc gcgtcctcct gggtgatggt ctcggtccgg
     7561 ctcatgtcaa tccccttgtc gagcacgacc atggcggtca ccagcttgct gatcgaggcg
     7621 atcggccgga cctcgtcggc ctctttcgca tagagcaccc ggccgttttc gtagtcgacc
     7681 aggatcgccg ccttggtgtt gagccgcggt ccctcccgca cggtcgagaa atcgaactgg
     7741 tagttgcgct ggagcggtga aagccccggc ccgtcctcgg cctccaggaa atagacggtg
     7801 ttggccgaga agaacacgaa tgcggcgagc atgatcagca cgctcggcag ggtagtgcgc
     7861 ttgacgatca ttccccaggt gtctccgctc tgtcggttgc cgctagtatc tcacccattt
     7921 gactatctcc ggcagcgtcg gccgcttgcc gtacatcagg attcccacgc ggaatatctt
     7981 cgccgtcagc cagatcaccg ccaccgtcgt cagcgacacc agcacgaagc cgagcaccgc
     8041 ctggccgacg attcccccga agagcgagta ctcggtcatg gtcggggcga tgaagatgat
     8101 ccgcatcatc atcatcgtgg gcgtgagaaa cggaatgtac gagagcacca gcgacagcgt
     8161 cgagttgggt tcctgcacga cgtggatccc gatcatgacc ggcagcacga gcgacatggt
     8221 gatcgggaac acgaaattct gcgcttcctt ctcggaattg acgatcgatc cgaccagcgc
     8281 gaagagcgtc gagaacagca ggtacccggt cgtcaggtag agcacgaaga agaccacgat
     8341 gaccgggttg aacaggagcc ggtcgatcga gctgtcgacg ctgagcgcgg ccttgtttgc
     8401 atacagcacg gcgccgagcg ccacccagat cgccgcctgc gtgaaggtcg ccgcccccag
     8461 ccccagcact ttcccgagca taagctggaa cggggtgacc gaggagatca ggacttccat
     8521 gatgcgcgag ttcttctcgt cgatcacgct ccgcatcacc atctggccgt atccgagaat
     8581 catcccgaac atgaccatga cgaagatgag cgcgccgaaa tacttgtact cgaacggcat
     8641 cgtctccccc ttcgcgtccc gcacctggag atccagccgg cgcgtcaggg cgagaacgga
     8701 atcgacactc acattgacgt gggactgccg cagccgctcc atcgacaaaa gctgcgaggc
     8761 gtgattctcg attgtctgga ggatggtgaa attggtggcg ttggtgaccg caaatacgcc
     8821 cgtgtcggac aactgggggt tcggccgaat caccaccacg tatttgagtt cctgggcgtt
     8881 gatcgccgcg gtgagcgaat cgagcacccg ccggtagccc gggtcgtccc cgggggcgac
     8941 cgtgaaaaag ccgcggaagt catacagggg ggcttcggtt tccttgatct tgacctcgac
     9001 cagccgctcc tggagccgct cgccgaatcc ggcgctgctc tggtcgatga tggcgatcga
     9061 ctggggccgg tccagccccg ggtcggccag cagcatcggg agcaccacga acccggccat
     9121 gagcgccggc gtgagcagca cgccgatgag aaacgatttc ttcttgacca cctgcgcgta
     9181 ctcgcgcttg aagacgaccc agaatttacg catgggcggc ctccccgccg gctgcggccg
     9241 cgtccgattc gggccgggcc atgcggatga agatgctgta gagggaaggt tcgattatct
     9301 gaaaccgccg caccggcacg cggtcggtca ccgcccggag aatctcgttg ggccgcgctc
     9361 cgtcccccag atcgatttcg atgtaattgg tgaactgggt gacattcctg acccccggca
     9421 gctctcccac aaagagggcg tccccctcga tttccagttg gatcgtgttc tcgccgaact
     9481 gcgctttcac ccggcccagg ggaccgtcga gcaccttctc cccgcccgcg atcatgcaca
     9541 cgtggtcgca cagcttctcg gcctgttcca tcacgtgggt cgaaaagaga atcgtccggc
     9601 cggagcgctt gatctccagg aggatgtcct tgatcagttc gatgttgatc gggtcgaggc
     9661 cggagaacag ctcgtcgagg atgacgattt ccggttcgtg gatgatggtg gtgacgaact
     9721 ggagtttctg cgccattccc ttggacagct cctcgacctt gcggtggcgg taggccgcca
     9781 ggttcatgcg ctccagccag gggccgattt tcggcagcat ctcgcgccgc ccgacccctt
     9841 tgagctcggc catgtagagg atgacctctt cgagcgtcat cttcttgtac agtccccgct
     9901 cttcgggaag atacccgacg cggttgttga aatcgggacc ggccgtgcgg ccgtcgacca
     9961 gcacccgccc ccggtccggg acggtgatat tcatgaccat acggatggtc gttgtcttgc
    10021 ccgccccgtt gggcccgatg atgccgtaga tcaccccctc gggcacttcg agcgagagat
    10081 cgttgacggc caccttgccg tcaaattcct tgcgaatatg atcgagcgtc aactgcatcc
    10141 gcttctcctg gtctcacaat ggctttggat acgcattccg cccggtcggc gtcaaccaaa
    10201 aaaccgtccc cggcggcctc acagccccaa tagttctctc atttcgacgg cgttggccgc
    10261 cgcctgtccc aggtggcagt tgttgaaata gacatacagc ttcgcggcct ctcccttggc
    10321 cgccagtttg ccgatcttct gcttccactc ctccagttgt tcgcgcgagt agcggtagtc
    10381 atagcgcagc gctcccccct cccaccagta ctcggcgttg cggccgtgga gccgcaggta
    10441 ggcggtcggc ccggtcgtga acagatccgg cttgatcagg ttgggcagtt ccggttcgtc
    10501 cacgcagacg tagccgatcc cttccgcccg gaggcggtca tacatggtac ggttcaccca
    10561 actccggtgg cggaactcgg tgaacagcgg ccggggggcg agggcggccc ggagcgcgcc
    10621 caggtagtcc aggctgtccg ggctgaaccg gaacgaatag ggaaactggg ccagcaggcc
    10681 ggacagcttc cccgacgcct ccatcggctc gaccgcctgc aggaactgcc ggagatcctc
    10741 ctcgatgtcc tgccgccggt gcgtgaccga ctgcggcagt ttcaccacaa agtcgaatcc
    10801 ctcgggcgcc ttcttcgcca gatttctcat caccgccggg tgcgggatac ggtagtaggt
    10861 ggagttgatc tcgacggtcg agaaccgctc cacgtagtag tccagcattt tccccttgtc
    10921 gatcgtctcc ggatagaagc tgcctctcca atcctcgaag ctgaatcccg atgtgccgat
    10981 gcgtatttcc acggttccgt ttccgctcct ttgggccggc tgccaagtgt ggtacgttgc
    11041 cgcccgcggc cggttgcggc ccggggcgac cggcccgcgc gccgcttcag cggtcgccgg
    11101 agggaggccg gcggtcggcc ggccgccgct ggaagcgcac acgtccgcac tttggacaca
    11161 cccacttgcg ctgcttgtgg aacgtccgtt tcagttcctt catcgtcgcc tggcagcggg
    11221 gacagcgcat ccggcccccg tcagctggtc aggcggagga agtcgctgag gaggaattcc
    11281 acccgcccga taaacgtcga catccgcgac atgaccgtgt agccgaaggt ggtcccgaag
    11341 aagatcatca ggaagtacac cccgatcttc gccgaccgcc ccagcgcccc tttgtgttcc
    11401 cggctgaaat agaaatacga gagggtggtc acgactccga ccatgaccac gatcgccgac
    11461 cagttgggca tcccggtcgg gccgaccagc ggcatcaccg tggccgacat ctgcttgagc
    11521 gtccgggcct cgagcatcac gggtatggcc acgcccgctc cggagccgat cagcaccgcc
    11581 atcgacagcc gcgaccagga tttggtgctc gggtggaacc gcccgaacat gagcagcccg
    11641 aacacgagcg gcgcgatcag gccgagccgc ccgtccccga acagcggccg cacgagcagg
    11701 tccatgagcg tgttggtcca cgtgatcacg aggaagtacc cgatcgccac cccgatcaaa
    11761 agcgactccg ccatccggta gaccggattg tcgcggtaca ggaaactcag gatcgccagc
    11821 gtgagcgcgc ccgcgaccgc cgtgccccaa tccatcagcc gccccctcct ttctcccgcc
    11881 gccgggtcgc aaagaagaca aggttcccga cgacgatgag cgcgaccacg tagaggtgcg
    11941 agaccgtctg ggccagcatc ccccgctctc ccccgccgcc gcgcgcgatg agctgttcgt
    12001 actcggccgc cccgcgcagc ccgccgacca tggcctgcaa ttgccccgag gccaggtacg
    12061 gatcataggt ggtcaccatc gcggccgtca ccgccgccgc cacccgcacg cggtagcggc
    12121 tggccgcgta ctcgatccag tgggtcgtca gcgacccgtc ggccaccgac acgactccgg
    12181 cgatctgacc ataggtctcg atctccgcca gcatcggaag gtccgtatac ggccgcccga
    12241 ggtaatcctc cggccaggtc ttcgcgaacg actcccccat cgagagaatc gccgcgatat
    12301 actgcggctt gaacccgagg tacacccagt cctcgccgta gcgcttgtca tactcccggg
    12361 ccgtctgctg caccaggcgg tagccgaccc ccgtcccctc ggccagtagg gcggtgccga
    12421 caatcttcag gttgcgcgag aaggcatgcc ggagcaccgc cagcgccagg ggccggattt
    12481 ccgggagcga ggaggcctcg tggtcgaacg acacgatgat cgtcgacccg gccggcacct
    12541 cctcgatgaa ccggtgcatc ccgagcgtat cgtcggaggc cggcacctgc agattgacct
    12601 gcagcgccat ggccgcgacc acggccgccc cgagaccgag gaagatccag tggcgcgatt
    12661 ccagcttcat cttccgcctc tccccagcca ggtgcgttcg atccccagaa tcacgcgcag
    12721 cgaggtggtg agcgacccga ggccgatgcc gatgagaatc gcccgccgcg ccgccatcga
    12781 gggtacatcg cgcacccact cagcgacccg gttcacccag tcggggtacc aatccccgag
    12841 cgccgagcgg gtgagaatcg tcaggaacgc ggccgccagg agcacagccg ccggcgccga
    12901 tcgcgcccgg aacccgcggt acgaggccga ggcgacgaaa aacgcaagga gcgcaaacac
    12961 agtcgcctgc ataggcacct ggacattctc aaagacccac atgaaggggg actcggcccg
    13021 gattccccac aaaaccgcca gcaccggcat gagcgcgacg cccgccagcg acaccgcgcg
    13081 gtagggccaa tcggccgcgc gtccaatccc ggccgtggcc gtccgcacat aactcaccac
    13141 ccccaccagc agcgtgaagg cgaaaataat ctgccagtaa tcgacgagca ccgcggagtt
    13201 gatgctccgg gccgccggcg catcggagaa gtactggagg aacatgactg cgccgaacac
    13261 cagcacgata aactggggaa gccgccgtcc catctacagc ctccaccacg tgctgtccgg
    13321 ggcgaaggtc gcccagacca ccgccgcaat gagcagcact acgatgagca ccttcagaat
    13381 gtcctgagcc cggacggagg cgatgatgga gggatcctcc gagaggtagc ccgaggccgc
    13441 gaacagctcc tcgccgatca gaacgtagtc gcaggccacg atgaagaagg ccagttggat
    13501 ggtggagtcg gtgccggcga tctggatgct cttgttgatg ttggccgtct ccgccagaat
    13561 cagtgactcg gcctcgaacg tccccaggag gaacaccgag gccggtttca gccggctgat
    13621 ctgcccctcg acggcggccg cgtatccgaa ctgcgacgaa ctgacatacg aggcgctgtc
    13681 ttcgcggaaa cggtcgggaa atcccgcccg ggcgcagctc tcgcgcatga cctcctgcgc
    13741 caccgccatg atgaccggat cgtgggtggg aaacaccagc cggcagtcgt actgggccgc
    13801 cttctcggcc acgtggctga ggatgttcag cgaggcgatg gtggtggggc gctggatgtc
    13861 tcccccccat cccggcgtgt agagaatggg ccgggccatc tcggtggccc gccccaccgc
    13921 ctcctcgatc gcccggagcc ccgcgatctc gcgcatccgg taccgggcgc ggcttttccg
    13981 gtggtaggtc gtataaagca cgatgacgat ggcgaccgcc gccgccacca gggtcacgct
    14041 cgtggaggcg tcgaaccagg gcggatcggc ggccgcagcg atgagtggaa ctgccgccat
    14101 gttcacggca catggaaaga cctccgcctg cgtcgaaaca actaaaagat tattttggtt
    14161 gaccggggac gccggcgatc cgtttcatgc cgctgcgaga cgacgtgtct ggatgcagag
    14221 gtaggtggat gagaatacgt gcgacgatcc ttgcggccgc gctccttctg gccggcccgg
    14281 cccgcgccgc cacggtggaa tggtggcagt tctggaccga cccggccatc aaaccggtca
    14341 tcgaacaaat ggtggccgag tttgaacagg ccaaccccga catcaaagtg cgcctgaccg
    14401 atctcacctg ggcgaacggc cacgagaaaa tcgtcctggc tttctcctcg ggcacggccc
    14461 cggacgtggt ggaactcggc tccgactgga tcgcccagtt cgccgccaac gggcggctcg
    14521 ccgatctttc cggcctggtc gccggcgaca ccgccgggta cgatggctgg agcatggccg
    14581 ctgaccgcgg cgggctgtat gcctggccct ggattctcgg cacccgcgtc ctcttcggca
    14641 accgcgacct gatcacccgc gccggctacg acactgcctt ctacccggtc aactggccgc
    14701 aactgacgga ggcggcgaag aaagtccggg cgctcggccc caacttctac ggttgggggt
    14761 ccaacacggc cgaaaagcac cgcctctata agaagtacct gccgtttttc tggtcggcag
    14821 gcgcccagtt gttttccgat gacggccgga tgtgcgtggt ggcctcctcg cgcgccgtca
    14881 gtgccctccg gacctaccag caattgcacg actccgccgg ctatgtcgac acccagcgcg
    14941 gtatcgagga cgccttcctc gacggacgca tcgggttcat catttccggg gactggctgc
    15001 tcaagcggat cgagctggag aaccggcgaa tcaacttgat atcgacgctg atccccggcc
    15061 cgaaattccc cgggcgctcg ttcctcggcg gcgagttcct ggccgtgagc gccgactccg
    15121 acgtgaagga ggctgccctg aggctgatcc gcttcgtcac ctccccggtc aaccaggtcc
    15181 gtttctgccg ggccaaccgt tcggccaacc cctcgagccg cgaggcccaa agggatccct
    15241 atttcgcctc caacccccac ctgcagacct tcatcgcagc cctcaagtcc gcccagcatc
    15301 cgccggtcga tcccgactgg gtttatatcg aggacattct cgagaaagcc gtggaggagg
    15361 cgctcttcgg gtcgcacctg gtggccgtgc cgctgctgga ggcccggtac aagatcgagg
    15421 agttgaaggg gctcaaatga ggcgctcgcg gctcaccgcc ctgctcctcc tgtccccctg
    15481 gattctcagc ttcgcggtgt tctggctgtt tcccctggtg tactcgctgg tgatggggtt
    15541 caccgactac cgtctcctcg ccccggcctg gcgctggatt ggcttggcca attaccggag
    15601 cctgctggcc gacgagagct tcctcgccgc ggtggagaac actttcatct ttgtcctcgg
    15661 gaccgtgccc gtcaccaccg tcatcgcgct cggcctcgct ctcctggtga accggcagtt
    15721 tcccggacgc ggcctcttcc gcgccggcta ttttattccc tcgatcacct ccatggttgt
    15781 cgtcgccctg attttcacca acctctacca acggggaggg tatatcgccg ccctcgccgc
    15841 gatggtcggc ctgccggcgc cggagcacgg tttcctattc agcgacaaga ccgcgctcgc
    15901 ctccatcatg gcgatggatg tctggatggc ggtcgggtac tacatgctca tctttctcgc
    15961 cggcctgaag tcgattcccg acgagttgta cgaggctgcc cgcatccagg gggccggccg
    16021 cgcgcgccag ttcttctcca tcaccctccc cctgctccgc ccggtcgcgc tcttcatcgt
    16081 cgtcatcaac tcgatcaagt cgttccaggt cttcgtcgag attttcgtca tgaccaaggg
    16141 caagttcgac accgcctcaa tggtatactt catctatgac gccgggctga ccaccgcctt
    16201 ccgcttcggt tacgcctcgg ccgccgccta cctcctgttc ctcatcatcg cggtcttctc
    16261 cgtggtgcag ttcgcgctcc tgcggcggag gcagccgctg tggtagcccg gagagccgcg
    16321 tccgccgtcc gctacgcggt gctcggcgtc atcctggcgg tgatggtgtt ccccctcgtg
    16381 tggatgttcc gggtggcggt gatgtcgccc ggcgccggcg tggggcttgc cgacctcttc
    16441 acgtccgatg tgacattccg caatttcgcc gacctgttcg cctccggccg catcggccgg
    16501 tacctcttca attcggcttt cgtcgcgctg gtggtcacgg ccggcaatat cctcttctgc
    16561 ttcatggcgg cctacgtgct cgcccgccgc ccggggctct ggacccgcgt gctctttgcc
    16621 tccgtcgtcg tcgttctcat gatccccgcc cacattctca tcgtgccgct ttacgtgctc
    16681 atgctcaagg cccgcctct
//
LOCUS       DAOPOE010000073        16137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4306_length_16137_cov_6.225967, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000073 DAOPOE010000000
VERSION     DAOPOE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16137
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4306_length_16137_cov_6.225967"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKM94_09975"
     CDS             <1..804
                     /locus_tag="PKM94_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD67006.1"
                     /translation="LLFYSAMALLVLVIAGRLDPARSRFSHALRLLYPVAILVPFYRE
                     TGGTMFLFFDHFLDAHLIAFETAVFGQEPTLFIDRHLLHPVLTEAFMFCYFCYYLVIP
                     VFMIAGYLRGRFEVVKSALAAVLLTFAVSYVVFFLFPIEGPRWRLTGQYLHPVVTGYV
                     FTDLVGLTQQYGSVRGGCFPSTHFAVTFVILLFTARYFRRGLWLMAPLTVGLGIGTFW
                     GRYHYVSDVAAGGLIGLAVTLLLWRAAPADRAGQMTEVRKLEAQTPHAS"
     gene            794..1636
                     /gene="lgt"
                     /locus_tag="PKM94_09980"
     CDS             794..1636
                     /gene="lgt"
                     /locus_tag="PKM94_09980"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOD67007.1"
                     /translation="MHPELFHVGPVHIRSYGLMLAISFLLGVLYIWRVARRDGKPFEP
                     LLTVAYLMTIGGIIGARLFYVLVHLDEFSGNWTRTFNPFSSSEFGIAGLNLYGGVLAA
                     IVASFVYLRAKKLSVLDTFDYFAPTLGLGLLFTRVGCFLNGCCFGTPTELPWGISFPE
                     GSLPYYIFSDAHLHPAQLYSSLYGGLLLIALHYVLRHRRFVGQAVALLFMIEAGFRYA
                     IEYVRYYEDEMHLTVGSMHPTYNQLVSLGLFVLGLGLYLYQRRRAPLSRAAAPPAGKL
                     RPAK"
     gene            complement(1709..3313)
                     /locus_tag="PKM94_09985"
     CDS             complement(1709..3313)
                     /locus_tag="PKM94_09985"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOD67008.1"
                     /translation="MSQPARVVPVILLAALLPIVIGSCSEDSVKSSPPELLVTPEAVS
                     GTASYGGSSPSGAWVQVRNGGDGELPFSAYAGKGTWCYLYYGSIVDTVGAAAPDSFAI
                     AFTTGSLEAGEHFDTVWIEAPAAANSPVAIPVHVTIANVVQVNPPNLVFRAVTGDVNP
                     PPQLVTILGASSTPLPFVLSSSQAWLQFSPAGGAAPTTVQVSVDLRGLPPGVYYDTIV
                     VQASSARTPGRIPVRLEVASWLRTSLSSSFDFFGIWFGDTQAGLAVGLLPTSGDGVIS
                     RTADGGNTWDSLVSTANEFFDGLGDVWASSGRAWAVGKNAFIYYSPDGGDTWLRRFAP
                     DEVTAALNGVHFADTLRGWAVGNDGVIIHTVDGGLTWEAQNSTTSRALASVWFADAAR
                     GWAVGNYGTILHTVDGGATWLPQANGLVQDDLWDLGFVSANEGWAVGDNGAVLHTVNG
                     GQDWEVQGRPSPASLRAVMFVNPLTGWAVGTGGVILRTDDGGEVWRLQASGVTSTLQD
                     VFFLNDQAGWICGSQGVLLRTSGGGD"
     gene            3575..4393
                     /locus_tag="PKM94_09990"
     CDS             3575..4393
                     /locus_tag="PKM94_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD67009.1"
                     /translation="MLHFVGYATVGLIIGTFGTIIGVGGGFLLVPLLLWLYPTAEPEV
                     LAGISLTAVFFNAFSGSLAYARLKRIDYRSGLTFSLAALPGTILGSLSTTYIPRHLFD
                     AVIGLLLLAASSYIVVRPNREKALRGGAVAGHTTVVLTDASGAQYRYAYNRRIGVGIS
                     FFVGYFSSLLGIGGGVIHVPALIQILNFPVHIGTATSHFILAVMTFAGSATHIVIGTL
                     ADSLRIILPISLGMIVGAQIGARLSGRVRGTWITRSLAVALMLVGIRTLWQAFK"
     gene            4538..5356
                     /locus_tag="PKM94_09995"
     CDS             4538..5356
                     /locus_tag="PKM94_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOD67010.1"
                     /translation="MQIENWFVGLSPVVQALMGTLFTWGLTALGAACVFLTRQPSRKL
                     LDAMLGFAAGVMIAASYWSLLAPAIAMAENKGVPAWVPATVGFLLGAAFLRSLDMTLP
                     HLHLFEKVEAAEGIKTRWHRTTLLVLAITLHNIPEGLAVGVAFGAVAAGLESATLGGA
                     IALALGIGIQNFPEGLAVSMPLRREGIGRGRAFWYGQLSGTVEPIAGVVGAALVLIAE
                     PILPYALAFAAGAMIFVVVEEVIPESQSGGHPHYATLGAMLGFAVMMTLDVALG"
     gene            5955..6407
                     /locus_tag="PKM94_10000"
     CDS             5955..6407
                     /locus_tag="PKM94_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD67011.1"
                     /translation="MRLKTAREILDFAIQKEQEAADFYNDLAERVDRPGMKSVFKGFA
                     KEELGHKAKLESVKAGNRMLAAEKKILDLKIGDHLVEVDLRPDLSYQEALIIAMKAEK
                     AAFKLYHDLAGTTDDPALREMLLGLAQEEAKHKLRFEIEYDDQILIEN"
     gene            complement(6580..7554)
                     /gene="hemB"
                     /locus_tag="PKM94_10005"
     CDS             complement(6580..7554)
                     /gene="hemB"
                     /locus_tag="PKM94_10005"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOD67012.1"
                     /translation="MAFPIHRPRRLRRTPALRRLVRETTLTPDDFIYPIFVSEVVKRA
                     EPIASMPGIFNFPVREAVDEARRAFTRGIPAVILFGTPAEKDATGSSSLAADGIVQQA
                     IREIKNALPEMVVVTDVCLCEYTDHGHCGVVEKGIVKNDESVEILAAQAVSHAAAGAD
                     MVAPSDMMDGRVGRIRNALDAAGHAMIPIMAYSAKYASAFYGPFREAAGSTPQFGDRR
                     GYQMDSANGREAMREIELDLAEGADIIMVKPALAYLDVIARARAAFPAPLAAYNVSGE
                     YSMVKLAAERGLADERTLTLEVLTSIKRAGADIIISYHAPDAAEWLGA"
     gene            complement(7554..9092)
                     /locus_tag="PKM94_10010"
     CDS             complement(7554..9092)
                     /locus_tag="PKM94_10010"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOD67013.1"
                     /translation="MTAGMVYLVGADPDDPELVAVKAQRLLASCDAVVYDEPVPHELI
                     TALPESAEKHYVGEAKAGVRRSESVGELLAHLARAGKTVVRLHGSDPLCLARAMDEAA
                     FLHRRGILWEIVPGSASTWAASACAGMDSTDTGRVSAVILIAGHGTTEERCADIDWDW
                     VAQAPRATIVLSAEPQDTAWIADRLIGGGMAPDTPASAGPLPDPSPRLITGTLADIAD
                     AVRRNRVTAPLGLHLGACAEPPSPRPEEDLRRPLRGLRILVTRAADQAAPMSRRLREL
                     GARVLALPTIATRAHFDPAAWERFEACTAERRWLVFTSENGVRHFSQQHRDRGLDVRG
                     LAAFRVAAVGSGTAEALRALSLLPDFVPGQATGSDLAKELAAHTDLRGAAVVRVRGNL
                     ASATPDAALAAAGAELIPMTVYETVHVVWPPGLKEMLAAAPPDAAIFTSGSGVDGLYA
                     NLTGEEISRIIAPGLVVSIGPSTSEAARARGLTVGLEAARHAIPDLIDELTEFVQTHP
                     IRRT"
     gene            complement(9142..10896)
                     /locus_tag="PKM94_10015"
     CDS             complement(9142..10896)
                     /locus_tag="PKM94_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD67014.1"
                     /translation="METIAVSGRSDVVVIGGAAAGPKTAATLARRRPDWRITLFEKGE
                     RVSFATCGLPWLAAGEIPSPDALVRTSWGAERSPEFFRDYKGFTAVTKAEVIALDRAR
                     RTVTVRRTATGETFAHGYDKLVLATGATPRTPPFPCCRSERVRHFTRPQDAIAFRRLA
                     EQGKIGEAVIIGAGLVGCELAEAAAGLWGIRTRVIERERHALPYALDEDMAVHVAGEL
                     ARNGVLLVTGATVRRVVEDDDRLAVEFLSGEDGTDARTCTGDFVFLCIGVQPETTLAR
                     TAGLAIGASGGLRVNEFLQTSDPHIYAAGDCIESTHLISRRPLSLPMGSLANRHGRVI
                     AEHMTGGEARLAGVAGGTAVKVFGLNVASVGLTERAAAEAGFAARAVTGSFSDRPDYH
                     PEHESVIAKLVYDPADRRLLGLQAVGAGEVVRRIDVFSSFLQRGAIVEDLLDFEHAYS
                     PPYAEALDPLHHLAAMAQAQERGVEFVGAVRALAEAPPDALWLDVRERGETAERPLAV
                     PGAPRRLLNLPLGELRAQLDSLDRRRDTYVVCRRGARAYQAALLLRSHGFERVHVVGG
                     GMAALEASISDGPDRDGN"
     gene            complement(10993..11310)
                     /locus_tag="PKM94_10020"
     CDS             complement(10993..11310)
                     /locus_tag="PKM94_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOD67015.1"
                     /translation="MATLTWEEVSISVDEDGFMEEPAKWDERVALALASTEGLTALTD
                     EHWKLITYLRDYYTQYGIAPMIRKLCKETGFPLKKIYELFPSGPAKGACKVAGLAKPT
                     GCV"
     gene            11966..12898
                     /locus_tag="PKM94_10025"
     CDS             11966..12898
                     /locus_tag="PKM94_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOD67016.1"
                     /translation="MSGLQLLFYFALAALVVGTGYKVVRIARMPLHLRWDLYPVPHER
                     GKGRYGGSYYEEVDWWTKKVDTTLVGEIREMAGEILALQSLFRHNRQLWIFSFPFHLG
                     LYLLVGFVLLLGLGGAIVASGGAVAPEGGGFGALVYYVTGVCGTAGALLVMLGAVGLF
                     VSRLASRDLRSASVRTDYFNLLLLSAVGATLAATGLGSDSGFHQARAFVQSLLTFSNA
                     PALSDLMRAHLILAAAFMCWLPLTHMTHFVGKYFTYHKVRWEDHPNIRGSRIEAAVAK
                     ALGYPLTWSAPHVGTGATWGEAAAGPDDEEKEQK"
     gene            12895..14511
                     /locus_tag="PKM94_10030"
     CDS             12895..14511
                     /locus_tag="PKM94_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD67017.1"
                     /translation="MSGRKVSIRDFCRKTGQLVELDPGRFLPLPEPYDRPEWNARLKP
                     LSEEARKKYEVDLDGVAAVKFPRPQTDAEKQELVEKFLAGLRKLFEKENNWTFMQPLF
                     LTMEYCARCQTCASACPIYTESGRLDIYRPTFRSDILRRLYRTYVKSGGKFTAKLTGH
                     DIEASWEMIARLMELSYRCTLCRRCAQTCPIGVDNGLVTHELRKIFSQEMGIAADSLH
                     KLGSVQQLAAGSSTGMTTAAFLDNIEFLEGEVEERTGRRFRWPIDKAGADVLLIHNAG
                     EFLAWPENPEAFAIILDAAGVSYTLSSEAVGYDSVNYGVWYDDVQFARVALRHAAIAK
                     KLGVKKIVLGECGHAHKALTVVADRIFVREYNVPRESALTFLHQILVKEKRLALDPSR
                     NDDILTTLHDPCNVVRLMGIVQPQRELLRAVSNRFREMEPHGVTNYCCGGGSGFAITQ
                     DLNFPDWRNSLAGRAKLRQILDVFQDVLDPGHKKYVCAPCSNCKGALRDMFAAYGLFE
                     RCSILYGGLVELIVNAMADVEKPFLEWEWR"
     gene            complement(14546..15487)
                     /locus_tag="PKM94_10035"
     CDS             complement(14546..15487)
                     /locus_tag="PKM94_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOD67018.1"
                     /translation="MSTSIQLINDLYKSINADPHQLQDPNVAHMEINYNRVLGMHAVP
                     GLTIDVNELSDGIEAQIALQEGAVVANPVHLCFGMLPKEGVQRIVLDVRIGVQAKVSL
                     LAHCTFPFAVNVQHLMNAAIEIKEGAEYAYFERHVHSPSGGVVVVPKAVVEVGPRARF
                     RTEFELIKGRVGNIDIDYEATCRAHSLLEMAARISGRGDDRIRINETAHLVGEHATGV
                     LTSKIAVRDTATAKVYNKLTAGAAYARGHVDCKEIIQDHGQAEAVPIVDVRHPKAHIT
                     HEAAIGSVDSKQLETLMSRGLSEDEAIEVIIGGLLSR"
     gene            complement(15484..>16137)
                     /locus_tag="PKM94_10040"
     CDS             complement(15484..>16137)
                     /locus_tag="PKM94_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67019.1"
                     /translation="VHAVVGPNGAGKSTLAATIMGLAGYDGYSGDIRFQGESIKSLPV
                     DQRARRGITLAWQEPARFEGLTVWEFLASAARDKSKDNLCRTLAEVGLNPEDYLRRAV
                     DKTLSGGERKKVELASILAMEPRVAILDEPDSGIDIESIERIFQAVKLLKQRGTTVIL
                     ITHSLAVLDQAEHAFLLCKGTVVDKGTVAKIRRYFEGKCMPCEHKNAPDRNGRGGES"
BASE COUNT         2539 a         5599 c         5113 g         2886 t
ORIGIN      
        1 ctgctgtttt attcggcgat ggcgcttctc gtgctggtga tcgccggccg gctggatccc
       61 gcccggagcc ggttctccca tgcgctgcgg ctgctctacc cggtggcgat cctggtcccg
      121 ttttaccggg agacgggcgg cacgatgttt ctcttttttg accactttct cgatgcccac
      181 ctgatcgctt tcgagacggc tgtcttcggg caggagccca cgctgtttat cgaccgccac
      241 ctcctccacc cggttctgac cgaggcgttc atgttctgct acttctgcta ctacctcgtc
      301 attcccgtgt tcatgatcgc cgggtacctg cggggccgct tcgaggtggt caagagcgcc
      361 ctggcggcgg tcctgctgac gttcgcggtc tcgtacgtgg tgttctttct gttcccgatc
      421 gagggtccgc gctggcgcct gaccggccag tacctgcacc cggtcgtcac cggttacgtc
      481 ttcaccgatt tggtggggct gacccagcag tacggctcgg tgcggggcgg gtgctttccc
      541 tcgacccact ttgccgtgac cttcgtgatc ctcctgttca cggcccggta tttccgccgc
      601 ggtttgtggc tgatggcgcc gctgacggtc gggctgggga tcggcacctt ttgggggcgg
      661 tatcactacg tgtcggacgt ggccgccggc gggctcatcg ggctcgccgt gaccctgctc
      721 ctctggcgcg cggccccggc cgaccgggcg gggcaaatga cagaagttcg aaaactggag
      781 gcacagacac cgcatgcatc ctgaactctt ccatgtcggg ccggttcaca ttcgcagcta
      841 cgggctcatg ctggccatct cgtttctgct gggggtgctc tacatctggc gggtcgcccg
      901 ccgcgacggg aaaccgttcg agccgctgct cacggtcgcc tatctcatga cgatcggcgg
      961 catcatcggc gcccggctgt tctacgtgct ggtccacctc gatgagtttt cggggaactg
     1021 gacgcggacg ttcaacccgt tttcctcgag cgaattcggc atcgccgggc tgaacctcta
     1081 cggcggcgtg ctggcggcca ttgtcgcctc gtttgtgtac ctgcgcgcga agaagctctc
     1141 ggtcctggac accttcgact acttcgcgcc gactctcgga ctcggcctgc tgttcacccg
     1201 ggtcgggtgc ttcctcaacg ggtgctgttt cgggacgccc accgaacttc cgtggggaat
     1261 cagctttccg gagggttcgc tgccgtacta cattttcagc gacgcccacc tgcacccggc
     1321 gcagttgtac agctctctct atggagggct gctcctgatt gcgctgcact acgttttgcg
     1381 gcaccgccga tttgtcggcc aggcggtcgc tctgctgttc atgatcgagg cgggcttccg
     1441 ctatgcgatc gagtatgtgc gctactatga agatgaaatg cacctgacgg taggatcaat
     1501 gcatccgacc tacaaccaac tcgtatcgct cgggctgttc gtgctcggtc tggggctgta
     1561 tctctaccag aggcgccggg cgccgctgtc gcgcgccgcc gccccgccgg ccggaaagct
     1621 cagaccggcg aagtagtccg cgcggggacc gcgccgaggg ccctcttatc ggcccgcctg
     1681 gcgaccgcgg gacggctccg gttttctttc aatccccgcc gccgctcgtg cgcaggagca
     1741 ctccctggga accgcagatc cagccggcct ggtcgttcag gaagaagacg tcctggaggg
     1801 tcgaagtgac tccgctcgcc tgcaggcgcc atacctctcc cccgtcgtcg gtgcgcagga
     1861 tgacgccgcc ggttccgacc gcccacccgg tgagcgggtt gacgaacatg accgcgcgga
     1921 ggctcgccgg ggagggccgc ccctggacct cccagtcctg cccgccgttg accgtgtgca
     1981 ggaccgcgcc gttgtcgccg accgcccacc cctcgttcgc actcacgaag cccaagtccc
     2041 agagatcgtc ctgcaccagg ccgttcgcct gcgggagcca ggtcgcgccg ccgtcgacgg
     2101 tgtggaggat ggtcccataa ttgccgaccg cccagccgcg cgcggcgtcc gcgaaccaga
     2161 cactcgcgag cgcccggctg gtcgtggagt tctgcgcctc ccacgtgagg ccgccgtcga
     2221 cggtgtgaat gatcacgccg tcgtttccga ccgcccaccc gcggagggtg tcggcgaaat
     2281 ggacgccgtt gagcgccgcc gtcacttcgt ccggggcgaa gcggcgaagc caggtgtccc
     2341 cgccgtcggg ggaataatag atgaacgcat tcttgccgac cgcccaggcg cgcccggacg
     2401 acgcccacac atcccccagg ccatcgaaga actcgttggc agtgctcacc agggaatccc
     2461 acgtgttccc gccgtccgcc gtccgcgaaa tcacgccgtc gccggatgtc ggcagcagcc
     2521 cgacggcaag tccggcctgc gtgtccccga accagatgcc gaagaagtca aacgaggaac
     2581 tcaggctggt gcgcagccaa ctggccacct cgaggcggac cgggatgcgc cccggcgtgc
     2641 gcgccgagga agcctggacg acgatcgtgt cgtagtacac tcccggcggc aggccccgga
     2701 gatcgaccga cacctggacc gtggtgggcg ccgctccccc ggccggggag aactggagcc
     2761 acgcctggga actcgaaagg acgaacggga gcggcgtcga gctggccccg aggatcgtga
     2821 ccagttgcgg cggcggattg acatcgccgg tcaccgcgcg gaacaccagg ttcggcgggt
     2881 tcacctgaac cacgttggcg atcgtcacgt gtaccgggat cgctaccggc gaattggcgg
     2941 ccgcgggggc ctcgatccat accgtgtcga aatgctcccc ggcctcgagg ctgccggtgg
     3001 tgaaagcgat ggcgaaactg tcgggggccg ccgctcccac ggtgtcgacg atcgacccgt
     3061 agtacaggta acaccaggtt cccttgccgg cataggcgga gaacgggagc tcgccgtcgc
     3121 cgccgttgcg cacctggacc cacgcgccgc tcgggctgga tccgccgtac gaggctgtcc
     3181 ccgacaccgc ttccggcgtg accagaagct ccggcggcga cgatttgacc gaatcctcgc
     3241 tgcaactgcc gatgacgatc ggcagaaggg cggcgaggag aatcaccgga acgacacggg
     3301 cgggttgtga catcacagtc tgctcctggt gcgatgagtc gcttgcatgc gtcaagaagt
     3361 ccccgacccc tgccaaagtc aaggatcttg ttctcctgac acggtcagtc ccatagtaaa
     3421 gacgcgggcc ggggggaaat gtttactccg gccgcccggt cctgggcgga ccggtgtccc
     3481 aggcgccctt tccacatcga aatctcggcc cgatcctccc cggcgcaaaa acaaaatcct
     3541 tgcctttccg gggttttccg gtgcacatat cgcaatgctg cactttgtcg gttacgcgac
     3601 cgtcggactc atcatcggca ccttcggcac cattatcggt gtgggcgggg ggttcctgct
     3661 tgtccccctg ctgctgtggt tatatccgac ggccgaaccg gaggtgctgg ccggcatctc
     3721 gctgaccgcg gtgtttttca acgctttttc cggatccctc gcctacgccc ggctcaagcg
     3781 gatcgactac cggtcgggcc tgactttttc tttggcggcg ctgcccggga ccattctcgg
     3841 atcgctgtcg acgacctaca tcccgcggca cctgtttgat gccgttatcg gccttctgct
     3901 gctggccgcg tccagttaca tcgtggtgcg ccccaatcgc gagaaggcgc tccgcggagg
     3961 cgccgtcgcg ggccatacga cggtcgtgtt gacggatgcc tcgggcgcac aatatcgcta
     4021 cgcctacaac cgccggatcg gcgtgggcat cagcttcttc gtcggctatt tctccagcct
     4081 cctgggcatc ggcggcgggg tcattcatgt cccggccctc atccagatcc tgaattttcc
     4141 cgtccacatc ggcaccgcca cctcccattt cattctggcc gtcatgactt tcgcgggaag
     4201 cgccactcac attgtcatag gcacgctggc cgattccctc cgcatcattc tgccgatttc
     4261 gctggggatg atcgtcggag cgcagatcgg cgcccggctg tcggggcggg tgcgggggac
     4321 ctggatcacc cggagccttg cggtggctct gatgcttgtc gggattcgga cgctgtggca
     4381 agctttcaag tagcagccgg ccgcggggag ggcgtgtttt cggctgcaaa tcccggttga
     4441 tctgaggggc cgcttccggt attatggccc cgacctgaac ggcccggcga caccgccggc
     4501 cgggcgaccc aatgccgacg agttgcgcgg aggacagatg cagatcgaga attggtttgt
     4561 gggacttagt ccggttgtcc aggctctcat gggcacgctc ttcacctggg gactcaccgc
     4621 cctcggagcc gcctgcgttt tcctcacccg gcagccgagc cggaagctgc tcgacgccat
     4681 gctcggcttc gccgccggcg tgatgatcgc ggccagctac tggtcattgc tggcgccggc
     4741 catcgcaatg gctgagaaca aaggcgttcc cgcatgggtg cccgccaccg tcgggttcct
     4801 cctgggggcg gccttcctgc gcagcctcga catgactctc ccccacctcc acctgttcga
     4861 gaaggtggaa gcggcggagg ggatcaagac ccgctggcac cggacgaccc tgctggtgct
     4921 ggccatcact ctccacaata tacccgaggg gcttgcagtc ggagtcgctt tcggcgcggt
     4981 ggccgccggc ctggagtcgg cgaccctggg cggcgcgatc gccctggccc tgggcatcgg
     5041 catccagaac ttccccgagg ggctcgcggt ctcgatgccg ctgcggcgcg agggaattgg
     5101 ccgcgggcgc gctttctggt acggtcagct gtccgggacg gtcgagccga tcgccggcgt
     5161 ggtcggcgcg gctctcgttc tcatcgccga gcccatcctt ccctacgccc tcgctttcgc
     5221 cgccggggcc atgattttcg tggtggtcga agaagtgatt cccgaatcgc agagcggcgg
     5281 ccacccgcac tacgccacgc tcggcgccat gctcgggttc gcggtcatga tgacgctcga
     5341 cgtcgccctc ggatagaggc gcgaccccgg ccctccgagc gcaaacaaca acggccgcgg
     5401 gagcgcggcc gtaagcggtc gggcccgggc gggcccgacc ctttggaatc gcaaagttta
     5461 cttgcccttc ttgccgccct tgggcgactt cttcgcggcc ggcttcttgg tcgcggcctt
     5521 cttcgcggcg ggcttcttag cgcacggcgc catgatgaga actcctcgga atggtgttgt
     5581 cgttgtggtc tcgaatactc gagaaccgct gcgtacacgc agccagaccg cgcgcggcct
     5641 gactcacctt gaggccgcgt gcgacttcca tcgccggacg gcctttgcgc cagacattac
     5701 acaatccaca atccagagtc aagtttcaaa caggcggcgg cgcaagaaat ttggcccgcc
     5761 gggacgcggt ccccggcagc ggatctcgga gacccttccc ggatccgccg ggtgagcaga
     5821 gtatctcgcg acggcctccg cccccggggc ccgcccgggc gaactagcca ttgcctccgg
     5881 cccgcccctg tactatactg ccggcgaaaa tcaacgagcc agccaggctc gggacgaccg
     5941 gcaggaggac atgcatgaga ctcaagaccg cacgagaaat cctcgatttc gccatccaga
     6001 aggagcagga ggccgcggat ttctacaacg atctggccga gcgggtcgac cgccccggca
     6061 tgaagagcgt cttcaaaggc ttcgccaagg aggaactggg ccacaaggcg aagctcgaat
     6121 cggtcaaagc cggcaaccgc atgctcgcgg cggagaagaa gatcctcgac ctcaagatcg
     6181 gcgaccacct ggtcgaggtc gacctgcgcc ctgacctgag ttaccaggag gcgctcatca
     6241 ttgcgatgaa ggccgagaaa gccgccttca agctctacca cgacctcgcc ggcacgaccg
     6301 atgatcccgc gctgcgggag atgttgctcg gcctggccca ggaggaggcg aagcacaagc
     6361 tccgtttcga gatcgagtac gacgatcaga tcctgatcga gaactgagct gggagcacga
     6421 gcgctttcga aaacggaccg cgccgggcac ggcaacaccg gcgttaggat ccgtcaaacc
     6481 gtaacgggat gcgatcgggt caatgccgcc gccaggcacg gagcaggccg acgcaagtcg
     6541 gtcgcaccgc cggcgtgggc cgggcgggag cggcgctgtt catgccccca gccactcggc
     6601 cgcatcgggg gcgtggtatg aaatgatgat gtcggccccg gcccgcttga tcgaggtgag
     6661 cacctccagc gtcagcgtcc gctcatcggc gagaccgcgt tcggccgcga gcttgaccat
     6721 gctgtactcc ccgctcacgt tgtaggccgc aagcggcgcg gggaaggccg cccgcgctcg
     6781 ggcgatgacg tccagatacg ccagcgccgg cttgaccatg atgatgtcgg cgccctccgc
     6841 gagatcgagc tcgatctccc gcatcgcctc ccggccgttg gcggagtcca tctggtagcc
     6901 gcgccggtcg ccgaactgcg gcgtggaacc ggccgcttcg cggaacgggc catagaaggc
     6961 cgaggcgtat ttcgcggagt aggccatgat ggggatcatg gcgtggccgg ccgcatccag
     7021 cgcgttgcgg atccgcccca cccggccgtc catcatgtcg ctcggcgcca ccatgtcggc
     7081 gcctgcggcc gcgtggctca ccgcctgcgc cgcgagaatt tccaccgatt cgtcgttctt
     7141 gacaattccc ttctcgacca ctccgcagtg cccgtggtcg gtatactcgc acaagcagac
     7201 gtcggtgacc accaccatct cggggagcgc gttcttgatt tcccggatcg cctgctgaac
     7261 gatgccgtcg gccgccagcg acgacgaccc ggtggcgtcc ttttccgccg gcgtgccgaa
     7321 gaggatgacg gccgggatgc cgcgcgtgaa ggcgcgccgg gcctcgtcca ccgcctccct
     7381 gaccgggaaa ttgaagatcc ccggcatgct ggcgatcggc tccgcccgct tgacgacctc
     7441 gctgacgaag atcgggtaga tgaagtcatc gggggtgagg gtcgtctcgc gcacgagccg
     7501 gcgcaacgcc ggggtgcggc ggaggcggcg gggacggtgg atcgggaaag ccatcaggtc
     7561 ctccgaatcg gatgcgtctg cacaaactcg gtcaattcat cgatcagatc cgggatcgca
     7621 tgccgggccg cttccagtcc gacggtcagc ccgcgcgcgc gggcagcctc ggaggtggag
     7681 gggccgatcg acacgaccaa tcccggcgca attatccggc ttatttcctc tcccgtcaag
     7741 ttcgcataca gaccgtccac gccgctgccg ctggtgaaga tcgccgcatc gggcggcgcc
     7801 gccgcgagca tttctttcag ccccgggggc cagactacgt ggaccgtttc gtacaccgtc
     7861 atgggaatga gctccgctcc ggcggcggcc agcgcggcgt cgggcgtcgc ggaggcgaga
     7921 ttcccccgca cgcgcacgac cgcagccccg cgcaggtcgg tatgcgccgc cagttccttc
     7981 gccaaatcgc tccccgtggc ctgccccggc acaaaatcgg gaagcagact gagagcgcgg
     8041 agcgcctccg ccgttccgct gcccaccgcc gccacgcgga aagccgcgag accccggacg
     8101 tcgagccctc gatcgcggtg ctgttggctg aaatggcgga cgccgttctc gctggtaaac
     8161 accagccacc gccgttccgc ggtgcaggcc tcgaaccgct cccacgccgc cgggtcgaaa
     8221 tgggcgcggg tggcgattgt cggcagcgcc agcacgcggg ccccgagctc ccgcagccga
     8281 cgggacatcg gggccgcctg atcggcggcc cgagtgacca gaatgcgcaa tccccgaagc
     8341 gggcggcgga gatcctcctc cggccgcggg gagggcggtt cggcgcacgc gccgaggtga
     8401 agccccagcg gggctgtgac cctgtttcgc cgcactgcgt ccgcaatgtc ggcgagggtc
     8461 ccggtgatga ggcgcggcga ggggtccggg agcggtccgg ccgaagccgg cgtatcgggc
     8521 gccatgccgc cgccgatcag ccggtcggct atccacgctg tgtcctgcgg ctccgcagaa
     8581 agcacgatgg tcgcccgcgg cgcctgggcc acccagtccc aatcgatgtc cgcgcagcgc
     8641 tcttctgtcg tcccgtgccc ggcgatcagg atcaccgccg acacgcggcc tgtgtcggtc
     8701 gagtccatcc ccgcgcaggc cgaggcggcc caggtcgatg cggatcccgg cacgatttcc
     8761 cacagaatgc cgcggcggtg caggaaggcg gcctcgtcca tggcccgtgc gaggcagagc
     8821 ggatcgctcc cgtgcagccg caccaccgtc ttccccgcgc gcgccaggtg cgccagcagc
     8881 tcgccgaccg actctgagcg gcggactccc gccttcgcct ctccgacata gtgcttctcg
     8941 gccgattccg gcagcgccgt aatcaactcg tgcggaaccg gttcatcata caccacggca
     9001 tcgcacgagg cgagcagccg ttgcgctttc accgcgacca gttccggatc atcgggatcg
     9061 gcgccgacga gatacaccat accggccgtc atttcacctc gttaagcttc atcgccattt
     9121 tcagccgcgt gggcgttccc ttcagttacc gtcccggtcc ggtccgtccg aaatggaggc
     9181 ctccagagcc gccattccgc cgccgaccac atgcacccgc tcgaatccgt gcgaccgcag
     9241 caggagcgcg gcctgatagg cccgcgctcc gcggcggcac acgacgtatg tatcccgcct
     9301 gcggtccaag ctgtcaagct gcgcgcggag ttccccgagc gggaggttga ggaggcgccg
     9361 aggcgctccg ggcaccgcga gcggcctctc cgccgtctct ccgcgctccc gcacatcgag
     9421 ccagagcgca tcgggcggcg cctccgcgag cgcccggacg gcgccgacaa actccactcc
     9481 ccgctcctgc gcctgggcca tcgcggccag gtggtggagc ggatccagcg cttcggcgta
     9541 gggcggagaa taggcatgtt cgaaatccag cagatcctcc acgatggcgc cgcgctgcag
     9601 gaaactcgaa aagacgtcga tccgccgcac cacttctccc gcgccgactg cctgcagccc
     9661 gagcaggcgc cggtcggccg gatcgtagac gagtttggcg atcaccgact cgtgctcggg
     9721 gtggtagtcc gggcggtccg aaaacgaccc ggtcaccgcc cgggccgcaa atcccgcctc
     9781 ggccgccgcc cgctcggtca gcccgacgga ggctacgttg aggccgaaca ccttgaccgc
     9841 cgtccccccc gccactccgg ccaaccttgc ctcgccgccg gtcatgtgct cggcgatcac
     9901 ccgcccgtgt cgattcgcca gcgaccccat gggcagggac aacggccgcc gcgagatgag
     9961 gtgggtcgac tcgatgcaat cgccggccgc gtaaatatgc ggatccgatg tctgtaagaa
    10021 ttcgttcacc cgtaggccgc cgcttgcgcc gatagccagc ccggccgtcc gcgccagcgt
    10081 cgtctccggt tgcacgccga tacagaggaa gacgaaatcg ccggtacagg tgcgggcgtc
    10141 cgttccgtcc tcgcctgaga ggaactcgac cgcaagccgg tcgtcatcct cgaccacccg
    10201 ccgcacggtc gctccggtca ccagtagcac gccgttgcgg gccagttcgc ccgcaacatg
    10261 aaccgccatg tcctcgtcga gcgcataggg cagggcgtgg cgttcgcgct cgatcacccg
    10321 cgtgcggatt ccccagaggc cggccgccgc ctccgccagt tcacacccga ccaaacccgc
    10381 cccgattatc accgcctccc cgatcttgcc ctgttccgcg agacggcgga aggcgatcgc
    10441 atcctgcggc cgcgtgaaat gccgcacccg ctcgcttcgg cagcagggaa acggaggggt
    10501 gcgcggcgtg gcgccagtgg cgaggacgag cttgtcgtag ccgtgcgcga aggtctcccc
    10561 ggtcgccgtt cggcgcaccg tcaccgtcct gcgcgcgcgg tcgagggcga ttacctcggc
    10621 ctttgtcacc gccgtgaaac ccttgtagtc gcggaagaac tcaggcgagc gttccgcccc
    10681 ccaggaggtg cggacgaggg catcgggcga ggggatctct ccggcggcca gccacggcag
    10741 accgcaggtc gcaaacgaca cccgctcccc cttctcgaac agggtaatcc gccagtccgg
    10801 gcggcgccgc gcgagagtcg cggccgtttt gggacccgcc gcggccccgc cgatcacgac
    10861 gacatccgac cgtccagaga ctgctatcgt ttccacgcat gtcagggccg gcctacgcca
    10921 cccctggagt cttctcctct ctccggcccg gctcgccggc gctgcggctg cgggagggcc
    10981 cggcgatcgc gctcacacac aaccggtcgg tttggccagc ccggccacct tgcaggcccc
    11041 cttggccgga cccgagggga acagctcata gatcttcttt agcggaaagc cggtttcttt
    11101 gcacagctta cggatcatcg gcgcgatgcc gtactgggtg tagtagtccc gcagataagt
    11161 gatcagcttc cagtgttcat cggtcagggc ggtcagtcct tcggttgacg ccagcgcgag
    11221 cgcaacccgc tcgtcccact tcgccggctc ttccatgaat ccatcctcat cgactgaaat
    11281 cgagacctct tcccaggtga gtgtcgccat tgtcgtcctc cgctgcctgt caattatatc
    11341 cgcacaccat catttgttca agctctcgag gctttgcacg cctcccaata tacctgcccg
    11401 tgcggccttc cgcacacttt ttgcaccatc gccgtcggcg cctgcaccgt gtctccgcca
    11461 tccgcagtca gggccgcaga cgcgaagtcc gggggcgcca ccgggcggga gacccggccg
    11521 cccgaaacgg ccgctgcatc ctctcgcata cccgtccggg cgtgcggccg ttcgtgaccc
    11581 ggcaatataa tatgcgcgtg gctgaggggc taatctgatt gagtcgagac cgcgggccga
    11641 tccggttcgc cggacgcgag ctaaccaagt tcgccttgac ggcgctgcgg agggaatcta
    11701 tatttgaccg tcttttttgc ggagaacccg gcggtgtgcc cgtggtacgc cccgaatgcg
    11761 gcccgggaga gacacttcac agattcgtcg gccgtcccga cacgtcccgg gacaggaatg
    11821 tgacctttcc ccgccccttc gccgcctgac ccccaggttc cagggccatc gccggcccgg
    11881 ccccggtcag cggagccctt ccgatcgagc ccggtgaatg gagcggcgcg ggattaccga
    11941 acccgggggg gctgaggaga acccgatgag cggtctgcaa ctgctgtttt acttcgccct
    12001 cgccgcgctt gtcgttggaa cgggctacaa ggtggttcgg atcgcccgca tgcctctcca
    12061 cctgcgctgg gacctctacc ccgtgccgca cgaacgcggc aaggggcgct acggtggctc
    12121 ctactatgag gaagtcgact ggtggacgaa gaaagtcgac accaccctgg tcggcgagat
    12181 ccgagagatg gccggggaga tcctggccct tcagtcgctt ttccgccaca accgtcagct
    12241 ctggatcttc tccttcccct tccacctcgg gctgtacctg ctcgttggct tcgtcctgct
    12301 tctcgggctg ggcggcgcga ttgttgcctc cggcggtgcg gttgcgcctg agggcggcgg
    12361 gttcggcgct ctcgtgtact acgtcaccgg cgtgtgcggg acggcggggg cgctgctggt
    12421 gatgctgggc gctgtcgggc tttttgtcag ccgcctggcc agccgcgacc tgcgctccgc
    12481 ctcggtgcgc acggactatt tcaacctcct gctcttgagc gccgtcgggg ccacccttgc
    12541 ggcgacgggg ctgggatccg actccggttt ccaccaggcc cgcgccttcg tccaaagcct
    12601 gctcaccttc tcgaatgccc cggcgctctc cgacctaatg agggcccacc tgatcctcgc
    12661 cgccgctttc atgtgctggc ttcccctgac ccacatgacc catttcgtgg gcaagtactt
    12721 cacctaccac aaggtgcgct gggaggatca cccgaacatc cgcgggagca gaattgaagc
    12781 ggcggtcgcc aaggcgcttg gctacccgct cacctggtcc gccccgcacg tcgggaccgg
    12841 cgccacctgg ggcgaggccg ccgcaggccc ggacgatgag gagaaggagc agaagtgagc
    12901 gggcggaaag tgtcgatccg ggatttctgt cggaaaaccg gccaactggt cgaactcgat
    12961 cccggccgat tcctccctct tcccgagccc tacgacaggc cggaatggaa cgcgcgcctc
    13021 aaaccgctct cggaggaggc gaggaagaag tacgaggtcg atctcgacgg cgtcgcagcg
    13081 gtgaaattcc cccgcccgca gaccgacgcc gagaagcagg aactcgtgga gaaattcctg
    13141 gccggtctcc ggaagttgtt cgagaaagag aacaactgga ctttcatgca accgctcttt
    13201 ctcacgatgg agtactgcgc gcggtgccag acctgtgcct cggcctgccc gatttacacg
    13261 gaatcggggc ggctcgacat ctatcgcccg actttccgct ccgacatact tcgccggctc
    13321 taccgcacgt atgtcaagtc gggagggaag ttcacggcga aactcaccgg ccacgacatt
    13381 gaggcttcct gggagatgat cgcccggctc atggagctct cctaccgctg cactctctgc
    13441 cgccgctgcg cccagacctg cccgatcggg gtcgacaacg gtctggtgac gcacgagctg
    13501 cgcaagatat tcagccagga gatgggtatt gcggccgact cgctgcacaa actcgggtcc
    13561 gtgcagcaac tggcggccgg ttcctcgacc ggcatgacga cggctgcctt cctcgacaac
    13621 atcgaatttc tcgaaggcga ggttgaggag cggaccggcc ggcgcttccg ctggccgatc
    13681 gacaaagccg gcgccgacgt gctcctcatc cacaacgccg gcgaattcct cgcctggccg
    13741 gagaaccccg aggctttcgc gatcatcctc gacgccgccg gcgtcagcta cacgctctcc
    13801 agcgaggcgg tcgggtacga cagcgtcaac tacggcgtct ggtacgacga cgtgcagttc
    13861 gcgcgggtcg cgcttcgcca cgccgcgatc gcgaagaaac tgggggtgaa gaaaatcgtg
    13921 ctcggcgaat gcggccatgc ccacaaggcc ctcaccgtcg tcgccgaccg catcttcgtc
    13981 cgggagtaca acgtcccccg cgagagcgcg ctcaccttcc tgcaccagat tcttgtcaag
    14041 gagaaacggc ttgcgctcga tccgtcccgc aacgacgaca tcctgaccac cctccatgat
    14101 ccctgcaacg tggtccgcct catgggcatc gtgcagcccc agcgcgaact cctccgcgcg
    14161 gtgagcaacc ggtttcgcga aatggagccc cacggcgtca ccaactactg ctgcggcggc
    14221 gggagcggat tcgccatcac ccaggacctc aacttcccgg actggcgcaa ctccctggcc
    14281 ggccgggcca agctgcggca gatccttgac gtcttccagg acgtcctcga ccccgggcac
    14341 aagaagtacg tgtgcgctcc ctgctcgaac tgcaaggggg cgctgcggga catgttcgcg
    14401 gcttacggat tgttcgaacg ctgcagcatc ctctacggcg gactcgtcga actgatcgtc
    14461 aacgccatgg ccgatgtcga gaaaccgttc ctcgagtggg agtggcgctg agccgcgccg
    14521 gcttgtgccg cgcgccgcgg tcgggttagc gcgacagcag cccgccgata atcacctcga
    14581 tcgcctcatc ctccgacagc ccccgcgaca tgagcgtctc gagttgtttg ctgtcgacgc
    14641 ttccgatggc ggcctcgtgc gtgatgtgcg ctttcggatg ccgtacgtcg acgatcggca
    14701 cggcctcggc ctgcccgtgg tcctggatga tctctttgca atcgacatgc ccccgcgcgt
    14761 aggccgcgcc ggccgtgagc ttgttgtaca ctttcgccgt cgccgtgtcc cgcaccgcga
    14821 tcttcgaggt cagcaccccg gtcgcgtgtt cgccgaccag gtgggcggtc tcgttgatcc
    14881 gaatccggtc gtccccccgt ccggagatgc gcgccgccat ttccaggagg ctgtgcgcgc
    14941 ggcaggtggc ttcgtagtcg atgtcgatat tcccgacccg tcccttgatc agctcaaact
    15001 ccgtccggaa ccgcgcgcgc ggcccgacct cgaccaccgc cttggggacc acgaccacgc
    15061 cgccgctcgg gctgtggacg tggcgttcga agtaggcata ctccgccccc tctttgattt
    15121 cgatggccgc gttcatgagg tgctgcacgt tcaccgcgaa ggggaaggtg cagtgggcga
    15181 gcagcgatac tttcgcctgg acgccgattc tcacgtcgag cacgatccgc tgcacgccct
    15241 ccttgggcag catgccgaaa cacaggtgca ccggattggc gaccaccgcc ccctcctgca
    15301 gcgcgatctg cgcctcgatc ccgtcgctga gctcgttgac atcgatcgtg agcccgggca
    15361 ccgcgtgcat ccccagcacg cggttgtagt tgatctccat gtgcgcgacg ttcggatcct
    15421 gcagttggtg cgggtcggcg ttgatgctct tgtagaggtc gttgatgagc tgtatggagg
    15481 tgctcatgat tcaccccccc tgccgttccg gtcgggggcg ttcttgtgct cgcagggcat
    15541 gcacttcccc tcgaagtagc ggcggatttt cgccaccgtc cccttgtcca caaccgtccc
    15601 cttgcacagc aagaacgcgt gttcggcctg gtcgagcacg gccaggctgt gggtgatcag
    15661 aatgacggtc gtgccgcgct gcttgagcag cttgaccgcc tggaagatgc gctcgatcga
    15721 ctcgatgtcg attcccgagt cgggctcgtc gaggatcgcc actctcggct ccatggcgag
    15781 aatcgaggcc aattccacct tcttgcgctc cccgccgctg agcgtcttgt cgaccgcgcg
    15841 gcgaaggtaa tcctccgggt tgaggccgac ctcggccagc gtccggcaca agttgtcctt
    15901 gctcttgtcc cgggccgcgc tggccaggaa ttcccacacg gtcagtccct caaaccgggc
    15961 cggttcctgc caggccagcg tgattccccg gcgcgcccgc tggtcgaccg gcaggctctt
    16021 gatcgattcc ccctgaaagc gaatgtcgcc cgagtagccg tcataccccg ccaggcccat
    16081 gatggttgcg gccagcgtgg atttgcccgc cccgttcggt ccgaccactg cgtggac
//
LOCUS       DAOPOE010000074        15982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4358_length_15982_cov_4.978841, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000074 DAOPOE010000000
VERSION     DAOPOE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_15982_cov_4.978841"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKM94_10045"
     CDS             complement(<1..216)
                     /locus_tag="PKM94_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67020.1"
                     /translation="MPEQPPALTPGESLLYASAMVVGGYARGVLVASREGRPIKVDGN
                     PLHPDTLGASDAPMQASILDLYDPARSR"
     gene            215..427
                     /locus_tag="PKM94_10050"
     CDS             215..427
                     /locus_tag="PKM94_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOD67021.1"
                     /translation="MSYTMAFETERHDTDRAFDCHGLTVLVDAQALRYLAGITLDFKG
                     GLMGGGFNFSNPRAKRSCGCGSSFSV"
     gene            427..720
                     /locus_tag="PKM94_10055"
     CDS             427..720
                     /locus_tag="PKM94_10055"
                     /inference="COORDINATES: protein motif:HMM:NF022118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HOD67022.1"
                     /translation="MPLAILDPQEFTEQGMFTEDGFLARAESFDFSRFDGCHVLIRGC
                     GGIPVPPWAFMLLTARLVGRARSVRYGNEHDHITVYRAPGRPQKPVAPRNPND"
     gene            770..1090
                     /locus_tag="PKM94_10060"
     CDS             770..1090
                     /locus_tag="PKM94_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD67023.1"
                     /translation="MRRIRFLPIDITVEVPEGTSILDAALDHDIRIDHNCGGNCACST
                     CHVIINEGYDTLAAPTEDEEDMLDEAENLTDTSRLACQCRVTKDLVVTIPEKEPLWDK
                     DDTF"
     gene            complement(1382..1552)
                     /locus_tag="PKM94_10065"
                     /pseudo
     CDS             complement(1382..1552)
                     /locus_tag="PKM94_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(2486..3040)
                     /locus_tag="PKM94_10070"
     CDS             complement(2486..3040)
                     /locus_tag="PKM94_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67024.1"
                     /translation="MGSVRPAVLCGVVLLSAAVSAATLPSRAIVHDCPAELFAQQFSL
                     RVLDPAEAAALGGKSFSGDTLKLLVIAVEWLEPPEKRPSTYAVDTLRQLFFSRDLMPH
                     GSVADYFDEVSYGQLQVTGQVYGWFNNGSYVASGDSLLYLLAELDSLIDFTQYDGNGD
                     GRVDGVVFVVAGRNRPDTNCLTDI"
     gene            3286..4053
                     /locus_tag="PKM94_10075"
     CDS             3286..4053
                     /locus_tag="PKM94_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67025.1"
                     /translation="MTTRHHILVRASQRGYSLFEMILVIVIIGILAAITMRSLRGAGE
                     AARTEETRAEMDRLAWAIAGDPGLVSGGHRTDYGYVGDVGALPPTLEALAVNPGLATW
                     RGPYVHDDFKTSAGAPESSFRIDAWGRPYAYAGVAITSTGGRIPLTRRLAPDAGRLLR
                     NTVTVVVTDLADSPPGARYVDSLAVILTVPNGLGGLVERRRTPRPDGFAEFDSVPIGA
                     HLLRLVYTPAADTLRRRVAVEPGQHAHLDLQYPAEVW"
     gene            4055..4822
                     /locus_tag="PKM94_10080"
     CDS             4055..4822
                     /locus_tag="PKM94_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67026.1"
                     /translation="MGEACRDNRGMSLIELLVVIVVIGILVAAAMQAMTATLADLRRV
                     RAERDLEALARAIVGDPVRTAGGARWDFGFVGDNGAFPPDLAALRANPGGWVTWNGPY
                     LDPGFDQDTLRYRLDPWGAPYQYVGGLTVISTGSGAPLVQRLADDAADYLANTYEGLV
                     RDAADSVPSSRYRDSVSILLTVPNGAGAWTILLQHPDSAGRFAFSPLPVGTHLLRIVF
                     TPRADTLLRCVTVLPRHRSDPPDRYRFTEAWFSGGTP"
     gene            4819..5958
                     /locus_tag="PKM94_10085"
     CDS             4819..5958
                     /locus_tag="PKM94_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67027.1"
                     /translation="MTGLRTASAFTLIEVILVVVIAGLLATVALRSAGALRESARNEE
                     ARRQMERLAAAVAGDPELRSLGVRADFGYVGDLGALPPDLGALRDNPGLAAWSGPYLV
                     SPAAEDPADCTTDPWGVPYALSGVNLVSTGSGSPLVFPLAASADDLLRNSVAGVVLDA
                     DGTPPGALYADSLRVIFSHPDGLGGRASRTRIPDRGGYFAFDSVPVGNHDLTIIYLPD
                     ADTLRRFVSVTPGAAPYSEFFLGANLWSAQGMSGTGLEFVDNSDTLAGGQCSVLNFSV
                     TNTTSHPITVSALTFTWPGPTAYYRKIRWNGVDVYSNPARGSGDLAVFTAPQTLSGGA
                     TARITCDQFKQFPSGGGASVNVTGVAFTVTFSDGSVITFVADNCR"
     gene            5973..6749
                     /locus_tag="PKM94_10090"
     CDS             5973..6749
                     /locus_tag="PKM94_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67028.1"
                     /translation="MTPVSPHLKSPAGFTLIELVIVLVVIGIIGAVALRDMLRAIDDA
                     RLAQTRAELDQIAFAIAGNPALQADGARSDFGYVGDVGALPPDLAALVANPGLASWRG
                     PYLDFGIAPSDRPSFGSPLSDAWDVPYAYTSPPDGPVAVQSSGSGAPLTRLVAPSAAD
                     LLNNTVRGFITDADRHSPGPVYRDSLALLLSYPDGAGGVAAVACVPAADGSFAFTGIP
                     VGNHTLRVIFIPAADTLAVPLCVPPASELKLAVAYPADLW"
     gene            complement(6753..7901)
                     /locus_tag="PKM94_10095"
     CDS             complement(6753..7901)
                     /locus_tag="PKM94_10095"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOD67029.1"
                     /translation="MKLAVIGAGLMGRAAVYDFAQGADVEQIGVYDINPELAQQVASK
                     FGGGKAKAGRLDAGDEEAAAKVLSGYDAAISCVTYRYNPGLTRAAIRGKCHLVDLGGN
                     NDTVRIQLGMDGEAAAAGVVIVPDCGLAPGMVSVLVADGVSRMEQTESVRIRVGELPQ
                     SPRPPLNYQMLFSAEGLINEYWEPCLILEGGKKKTVNPMTAVEEIEFDGLGKFEAFYT
                     SGGTSTLPDTYEGKVEFLDYKTIRYPGHCALFRPMLELGLASRKAVTVDGAEVEPRGV
                     FRAVLDRSLSFNEPDMTLVRVTVDGTAAGQKRTVVYEAVDRQDSKAGLTSMMRMTSFP
                     AAVVTQMAGRGQIAAAGCKPQEVVVSPGPFIQQLRKRGINIEIGERTG"
     gene            8192..11335
                     /locus_tag="PKM94_10100"
     CDS             8192..11335
                     /locus_tag="PKM94_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67030.1"
                     /translation="MRCIRPLILGAAVALYAGASATADRPLGSAKQLSPGAVIGTSYW
                     DRQCNFATTRRVHLSTAAGRELVHHVWTFQPAPPVWERTAHYRLYDIAGGAYSPGVDI
                     PEGTRIRAGFGDMWITDDNRAVIAYQHYSDPDQRYHLWTAFDSAAGAGEFSNLVEIPD
                     SIDFYSLVNYQSPLWPHVAYVEGVHDTVLHIVAQESLQYPDSVAIFYFRRVGSDSDPT
                     AVWDYPPRVIDSNYNWGSHQLAATSDGRVAIAWTANVPCAGDPDTASGVGCSDFVFWE
                     NDLYFQVSNDQGLTWQNRVNVTRYSETPEHPYRAHWEISALFDHLGVLHMIWSAAAWP
                     RTAPGPGEVAQTASAIFHWSETNPTPTMVRETNWNQTVCGGGEGRPLNIGDISLAECD
                     GRLYTLFVQFNDYDAGIMSDCCDSNLTFYPDGAANGDLYICVSSDGGLTWDAARNLTS
                     TRTPGCRFDTPGNVCACESWPSVTPYGSNVSIDPSISGADVVVPAGGSDPGWYLDVQY
                     VLDPLPSSPMTQEGWRTETSIRWFRIACVDPIQEPDLAILPDSLAYPVYTPHCAERLV
                     PIVLSNSGVGEGHYSIIAEEFAGPAGWLGTTGFSGTVAAGAAADTGYVVLNPGGALCS
                     PGAIVRLAGRLRFDSNDPNSPHYVLIDLVAADTVCLPSADTLTTACVALQVFNDAGFG
                     GRGAGGVTMDYSMSGDCDPAAATYLSDASPIVIADTLVNWSVYGCDFLAPHGLVPLAS
                     ASGSGGGFRWSRSLATVRDSSLILETDWYAPLPPDSCAFLIQRLSLYAADALPHTVSL
                     GQLIDWNIPSDSGLRNGSDTLVDPAIIYQYGAEYHQDPGACRNSDRRMGAVFPLAMYR
                     RAALHEPLVADSSNLWHCAQTRATDACLDTARCGLDAAELELALSEPGRRLYSATVPE
                     STYTDLHTLVGFVSDLSLVPGETLVVYTGLATVADGDTAVLRGLADQARQWYCDHLVP
                     DPPGCGCCRLRGDVNRDSGVKVSDLTFLASYLFRGGPEALCPDEADATGDGEVKVSDL
                     TYLTAYLFRGGPAPIPC"
     gene            complement(11424..15962)
                     /locus_tag="PKM94_10105"
     CDS             complement(11424..15962)
                     /locus_tag="PKM94_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD67031.1"
                     /translation="MAVSVCGTTEVCVEASVTADTVLSYILPMEHVFSFNAETGEICF
                     TPDTAGVYTIVFGVVGECGFSDRDTTRVTVAFSSGPSVDLGDDRAVSLCGPGEVCVDV
                     EASAGDLTILPPAASYDAETGRICFPADTAGLYRLIAQVTDSCGAAVDTVNISVDLGQ
                     PPVIAPLPDTTIYLCYSQWICLPLAVTDPDGDLDTVITNRGTVTNDGRVCFVPYTGGQ
                     YEIIITAIDECGNRDVDTAVVTIKTDENVNIITPRDTTVFTCSLVDTFCFPVAGVPEG
                     AQVTVSGINTWWDAANQTVCFWSQCSNQNRVRLTVRTPCTTYQREFRVTVLCNSAPVV
                     FLPGDTSLALCGTETFCLPAAISDVNRNIQSITVTGGVYNPRTSRICVDLAGPGTYTV
                     TAVAVDSCGASDSDQMQITVLANRPPTVTVAAPDTALSQCQPQQVCLPVTIADPDGQP
                     VTVVSLTGGFTYNAAASEICFTPGAEGTYCGALVIADQCNLQDTANFCVTITTGGSVD
                     IDCPVGAPPAVVLCAPGEVCLPLEITGAGYSVWTNIGVWANNELCFTADTSGVYSVRV
                     IASAKCNGDTCEFNVPVSVAEPVSILCPPDTTVILCAPDTICLPYSASASVESVQASG
                     GAFIDNGTVCVPIPSGVGQKSITLIASGDCGADTCSFTITYETNRPPVIVLTSPPPVT
                     TCATANVCVRYQVADLDSNIVSVVAGAGVVGPDSICFSNLAVGTYQVIATVTDACGAT
                     GKDTVTIRVLAGQSATIICPTSPPVDTICALDTVCVAAPVSPAGATVTVTPVGWYNAG
                     TGQVCVPVSATGTINVRMIAAAPCGSDTCEFTVSIVRAEQVAVTAPATEDTLVCFSGP
                     ATICYPVTVTGTQVNVTVTSPAVYSAGRVCLPVSAPGTYPITLTAVGYCNTVSKTTNV
                     IVRADQSPVLTVPGLQTFERCADDTSRICIDGIFATDAESTPVLSRTCGAGSFTLAGT
                     DSGQVCFVPDTFGVYSFCFEATDGCHTVRDTMDVEVVEKPGCDVCVKIWLDGGDCTPV
                     GVTKTMDIYIEALTPIGGFDLLLAFDKSVLTLTGLTRAASLQAWEYFTYRLDDVNCGA
                     SCSNRFVRLVGIADMNNGAQHPPASALSPAGLFATLEFYVANDQSLGDQWVPVQFYWF
                     DCGDNAFSDPTGYNLMVDLRIFENDVLRWDEDDDALYPEAARPMGLGAPDDCFVPVPG
                     KPTPTRCVEFYNAGIHICHPDSLDDRGDVNLNNVPNEISDVVLFSNYFVYGLSVFTVN
                     IAGQIAATDVNADGLTLTVADLVYLIRVLVGDENPFPRLAPRGQDVILSALREEGRVT
                     ISSEAPGEIGGALLVYQVPRGAQVSNVAAIGAAAGMDVKWNVMDGQLRILLFKIGKAA
                     IPAGPQELVSVALEGSGELLLARAEVVDYQGQAYSVAATGGVLPSEFVLDQNFPNPFN
                     PSTVISFALANTADWTLAIYNVNGALVREYHGSDAGRVQVEWDGRAADGSRVASGVYL
                     YRLEAGEFNDTKKMILLK"
BASE COUNT         2608 a         5974 c         4664 g         2736 t
ORIGIN      
        1 gcgcgagcgc gccggatcgt acaggtcgag gatcgacgcc tgcatcggcg cgtcgctcgc
       61 gccgagcgtg tccgggtgca gcgggttgcc atccaccttg atcgggcgcc cctcgcggct
      121 cgcgaccagg acgccgcgcg cgtacccgcc gacgaccatc gcgctggcgt agagcagcga
      181 ctcgcccggc gtcagcgccg gcggctgttc cggcatgtcc tacaccatgg ccttcgaaac
      241 cgaacggcac gacaccgacc gcgccttcga ctgccacggt ctgaccgtcc tcgtcgacgc
      301 ccaggcgctc cgctaccttg ccggcattac cctcgatttc aagggcgggc tgatgggcgg
      361 cggcttcaac ttctccaacc cgcgcgccaa acgctcctgc ggctgcggct cgtcgttctc
      421 cgtctgatgc ccctcgccat cctcgacccc caggagttca ccgagcaggg catgttcacc
      481 gaggacggct tcctcgcgcg ggcggaaagc ttcgacttca gccgcttcga cggctgccat
      541 gtgctcatcc gcgggtgcgg gggcattccc gtccccccgt gggccttcat gctcctgacc
      601 gcccgcctcg tgggccgcgc ccgctccgtc cgctacggca acgaacacga ccacatcacc
      661 gtctaccggg ccccgggccg gccgcaaaag cccgttgcgc cgaggaaccc caatgactaa
      721 cttggcccct gacaaaatcc gagaggacgc gaaccgcaag gacagaccca tgcgcaggat
      781 ccggtttctg ccgatcgaca ttacggttga agttcccgaa ggcacctcca tcctcgatgc
      841 cgctctcgac catgacatcc gcatcgacca caactgcggc ggcaactgcg cctgctccac
      901 ctgccatgtc atcatcaacg agggctacga caccctcgcc gcgccgaccg aggatgagga
      961 agatatgctc gacgaagccg agaacctgac cgacacctcc cgcctcgctt gccagtgccg
     1021 cgtcaccaaa gacctcgtcg tcactattcc cgagaaggaa cccctctggg ataaagacga
     1081 caccttctga ataaaaccgc ccgaaatgcg cgcggcgagc tcctgtcgga ctcgccgttc
     1141 tgattgccgc tcaatgccgg tccgcctctc agggacacgc cggcggggcc gctccgcccc
     1201 ggaacagata cgaaatcagc atcgtcacgt ccgacacccg cggcacgccg tccccgttca
     1261 cgtcggcgcg cgccgggcaa ccggccgccg gcccgccgcg gaagagatgc ccgatcaagc
     1321 cggtcaggtc cgacaccgcc agcgttccgt ccccgccgaa atccccccgc accacgcagc
     1381 aacaggcgtc ccccaccccg tcgccgtcaa aatccgcctg gtccgggttg gccgcaagcg
     1441 ggcagttgtc gcagacatcc ccgagcccgt ccccgtccgc atccgcctgg tccggattcg
     1501 ccgccgcgtc gcagttgtcg aacgcctcca ggatcccgtc accgtccgca tcatagctca
     1561 gcggatgcgc cacgtgaagg tacatcctgt ccccgaccat cgaatccacg tgcacgagca
     1621 cgtccgaggg gcggtcgtaa ccgtcgctgg cggggctcgt cttcggcccg aacagatcct
     1681 ggcccggcgt ctcgctggag aagagcgccc ccttgcgcgt ctcgtaggga taccaccaat
     1741 cggccgagtc gctcacctgc ccgcccgggt tgtacgcggt gtcccgcgcc ggatcgtacc
     1801 ccgcatcgac cacccgcacc cggtagtgct catcccacgg cgtcccgtaa ttggtcgcca
     1861 tcgtctcgtc cacgtgggtc accagcagtc cccggtcgag cgaatcggga ccgagactca
     1921 gatgcgggaa cagccagatg ctgaagtccg agtccagctt gtcgaacttc cccggcgacc
     1981 gccggttgcg gtactccagg agaaaatact ccgtcccgtc cgcgctgatg ttcagtttgt
     2041 agagcgagga ctcggggtgc gtctcgatat cgtacaacac gagattgagg tgctcccctg
     2101 tgatcaccgc cggctcgatc caccccagct catgcttcat gtacccgcac aggtgcgtcg
     2161 gcacggtcgc gcccaggctc agcgccccgt acccatagtg ccccatcagg caccagtcca
     2221 tgaccgggtg gtcgttggcg tcgccgggcg tcatggccgc cgaatcggag tagagcagat
     2281 cgatgtcgaa caggtcgccc gccccgagca tgtgcgcgat ctcgtggctc gccacccgca
     2341 ccccgtgcag ttcgtcgccg atgatcactg cgggatcggc cgctgagcgc accggtttga
     2401 cctccgggat cgccgcgagc tgattcacgt acattccgtc gaaaggcccg aattcctcgc
     2461 ccggaggaaa cgtgatcgcc gccgttcata tgtccgtcag gcagttggtg tccggccggt
     2521 tccgccccgc caccacaaac accaccccgt ccacccgccc gtccccgttg ccgtcatact
     2581 gcgtgaagtc tatcagcgag tccagctccg cgagcaggta gagcagcgaa tctcccgaag
     2641 cgacatagga accgttgttg aaccatccgt agacctgccc ggtcacctgg agctgcccgt
     2701 aggacacctc gtcaaaatag tcggccaccg atccgtgggg catcaggtcg cgcgagaaga
     2761 acaactgccg cagcgtatcg actgcatagg tcgagggccg tttctccggc ggctccagcc
     2821 actccaccgc gatcacgaga agcttgagcg tgtcccccga gaacgacttt cctcccagcg
     2881 ccgccgcttc ggccggatcg aggacgcgca gactgaactg ctgcgcgaaa agctcggccg
     2941 ggcagtcgtg cacgatcgcg cgcgacggca acgtcgcggc cgacaccgcc gccgacaaca
     3001 gcacaacccc gcagagcacc gccggccgga ccgaacccat caacacctca cctgggccaa
     3061 ccgcctcaac caaaataaga taaatgctat cgacaatata ggacacatta ccgattccgc
     3121 aaccgcgttt ttggctccgc gcccttgcgg cgcgccgcag tccccgcccg ccgactcccg
     3181 taaacccgct ctaacttcct gccgataaca gagataggcc gagtgcgccg atccgtcggc
     3241 agagccgccg ccggcccctc ggaacctgac tagcagtcga acaaaatgac gacccggcac
     3301 cacattctcg tccgcgcctc gcagcgcgga tactccctct ttgagatgat cctcgtgatc
     3361 gtcatcatcg gcatcctcgc cgccatcacc atgcgctctc tccgtggcgc cggtgaggcg
     3421 gcccgcaccg aagagacgcg cgccgagatg gaccgccttg cctgggcgat cgccggcgat
     3481 cccggcctcg tctccggcgg ccaccgcacc gactacggtt acgtcggcga tgtcggcgcc
     3541 ctccccccga ccctcgaggc tctcgccgtc aaccccggcc tcgccacctg gcgcggcccg
     3601 tatgtgcacg atgacttcaa aacctctgcg ggggcgccgg agagcagctt ccgcatcgac
     3661 gcctgggggc gcccctacgc ctacgccggc gttgccatca cttcgaccgg cggacggatt
     3721 cccctcacgc gccgtcttgc tcccgatgcc ggccgccttc tgcgcaacac cgtcaccgtt
     3781 gtcgtcaccg acctggcgga ttctccaccc ggcgcccgct acgtcgattc cctggccgtc
     3841 attctcaccg ttcccaacgg cctcggcggc ctggttgagc gaaggcgcac accccgcccc
     3901 gacggtttcg ccgagttcga ttcggtgccg atcggcgccc acctgctgcg cctcgtttac
     3961 acaccagccg ccgacacgct ccgccgccgg gtcgcggtgg aaccggggca acacgcgcat
     4021 ctggacttgc agtatcccgc ggaggtctgg tagcatggga gaagcctgtc gggacaaccg
     4081 gggaatgagc ctgatcgaat tgctcgtcgt catcgtcgtc atcggcattc tggtcgcggc
     4141 cgccatgcag gccatgaccg ccaccctcgc cgaccttcgc cgcgttcgcg ccgagcggga
     4201 cctggaggcg cttgcccggg ctattgtcgg cgaccccgtc cgcaccgccg gcggcgcccg
     4261 ctgggatttc ggcttcgtcg gcgataacgg tgcgttcccg cccgacctgg ctgctctgcg
     4321 cgccaacccc ggcggctggg tcacctggaa cggcccctac ctcgatcccg gtttcgatca
     4381 ggacacgctg cgctaccgcc tcgacccctg gggagcgccc taccagtatg tcggcggcct
     4441 caccgtcatc agcaccggca gcggcgcccc gctcgttcaa cgtctcgccg acgacgccgc
     4501 cgactacctc gccaatacct acgagggact ggtccgcgat gccgccgact cggtcccctc
     4561 aagccgctac cgcgactcgg tttcaatcct gctcaccgtc cccaacggcg cgggcgcctg
     4621 gaccatcctc ctccagcatc ccgactcggc cggccgcttc gccttttccc cactcccggt
     4681 cggcacccac cttctccgca tcgttttcac cccccgggcc gacaccctcc tgcgctgcgt
     4741 gaccgtcctc ccgcgccatc gcagcgaccc gcccgaccgc taccgattca cagaggcctg
     4801 gttctccgga gggacgccat gaccggcctc cgcacggcct ccgccttcac cctcatcgag
     4861 gtcatcctcg tggtggttat cgccggcctc ctggccaccg tcgccctgcg ctccgcgggc
     4921 gctctccgcg agagcgcccg gaatgaagag gcccggcgcc agatggagcg cctcgccgcc
     4981 gccgtcgccg gcgatcccga actccgctcc ctcggcgtgc gggccgattt cggctacgtc
     5041 ggcgacctcg gcgccctccc gcccgacctc ggcgccctcc gcgacaaccc cggcctcgcc
     5101 gcctggagcg gcccctacct cgtcagcccc gccgccgagg atcccgccga ctgcaccacc
     5161 gacccctggg gcgtccccta tgcgctcagc ggcgtcaacc tcgtctcgac cggatccggc
     5221 tcccccctcg tcttccccct cgccgcctct gccgacgacc tcctgcgcaa ctcggtggcc
     5281 ggtgtggtcc tcgatgccga cggcactccc cccggcgccc tctacgccga ctccctgcgc
     5341 gtcattttct cccaccccga tggcctcggc gggcgggcct cccggacccg catccccgat
     5401 cgcggcggct acttcgcctt cgactccgtc cccgtcggta accacgacct caccatcatt
     5461 tatctccccg acgccgacac cctccgccgc ttcgtctccg tcacccccgg cgccgctccc
     5521 tacagcgagt tcttcctcgg cgccaacctg tggagcgcgc agggcatgtc gggcaccggc
     5581 ctcgagttcg tcgacaactc cgacaccctc gctggcggcc agtgcagcgt gctcaacttc
     5641 tccgtcacca acaccacctc ccatcctatc accgtctcgg ccctcacctt cacctggccc
     5701 ggccccaccg cctactaccg caaaatccgc tggaacggcg tcgacgtcta cagcaacccc
     5761 gcccgcggca gcggcgacct ggccgtcttc accgccccgc agactctcag cggcggcgcc
     5821 accgcccgca tcacctgtga ccagttcaag caattcccca gcggcggcgg cgcgagcgtc
     5881 aacgtcaccg gcgtcgcttt cactgtcacc ttctccgacg gctccgtcat caccttcgtc
     5941 gccgacaact gcaggtaagg agaaccgccg ccatgacgcc tgtttccccg cacctcaaat
     6001 cacccgccgg cttcaccctc atcgagctgg tcatcgtcct cgtcgtcatc ggcatcatcg
     6061 gtgccgtcgc tctccgcgac atgctccgcg ccatcgatga cgcccgcctg gcgcagaccc
     6121 gcgccgaact cgaccagatc gccttcgcca tcgccggcaa ccccgccctg caggccgatg
     6181 gcgcccggtc cgatttcggc tatgtcggcg acgtcggcgc gctccccccg gacctcgccg
     6241 ccctcgtcgc caaccccggc ctcgcctcct ggcgcggccc ctatcttgac ttcggaattg
     6301 ccccctctga ccgcccctct ttcggaagcc ccctgtccga tgcctgggac gtaccctacg
     6361 cctacacctc cccgccggac ggtccggtcg cggtccagtc cagcggttcc ggcgccccgc
     6421 tcacccgcct cgtcgccccc tcggccgccg acctcctcaa taacaccgtc cgcggcttca
     6481 tcaccgacgc cgaccgccac tcccccggcc ccgtctaccg cgactccctc gccctgctcc
     6541 tgtcctatcc cgatggcgcc gggggcgtcg ccgctgtcgc ctgtgttccc gccgccgatg
     6601 gctctttcgc cttcaccggt atccccgtcg gcaaccacac cctccgcgtc atcttcatcc
     6661 ccgccgccga cactctggct gtccccctct gtgttccgcc cgcctcggag ctcaaactcg
     6721 ccgtcgccta ccccgccgac ctctggtagc cctcaccccg tccgctcgcc gatctctata
     6781 ttgatccccc gcttccgcaa ctgctgaatg aacggccccg gcgacaccac cacctcctgc
     6841 ggtttgcacc ccgccgccgc gatttgcccc cgccccgcca tctgcgtgac caccgccgcc
     6901 ggaaacgacg tcatccgcat catcgacgtc agtcccgcct tgctgtcctg gcggtccacc
     6961 gcttcgtaca cgaccgtccg cttctgcccc gccgccgtcc cgtccaccgt cacccgcacc
     7021 agcgtcatgt ccggctcgtt gaaactcaga ctccggtcga gcaccgcccg gaacaccccg
     7081 cgcggctcga cctccgcccc gtccaccgtc accgccttcc gcgacgccag ccccagctcg
     7141 agcatcggcc gaaagagcgc gcagtgcccc ggataccgga ttgtcttgta atcgagaaac
     7201 tccaccttcc cctcgtacgt gtccggcagc gtcgatgtcc cccccgaggt gtagaacgct
     7261 tcgaacttcc ccagcccgtc gaactcaatc tcttccaccg ccgtcatcgg gttcaccgtc
     7321 ttcttcttcc ccccctcgag gatcaggcac ggctcccagt actcgttgat cagcccctcg
     7381 gccgaaaaga gcatctggta gttcagcggc ggccgcggcg actgcggcag ctctcccacc
     7441 cgtatccgca ccgattccgt ctgctccatc cggctcaccc cgtccgccac cagcaccgac
     7501 accatccccg gcgccagccc gcagtccggc acaatgacca ctcccgccgc cgcggcttcc
     7561 ccgtccatcc ccagttggat ccgcaccgtg tcgttgttcc ccccgagatc caccaggtgg
     7621 cacttccccc ggatcgccgc ccgcgtgaga cccgggttgt accggtaggt gacgcaggag
     7681 atcgcggcgt catacccgct cagaactttg gccgccgcct cttcgtcccc ggcgtccagc
     7741 cgccccgcct tggctttccc gcccccgaat ttcgatgcta cctgctgggc cagttccggg
     7801 ttgatgtcgt agacgccgat ctgttcgacg tcggctccct gcgcgaagtc atagacggcg
     7861 gcccggccca tgagcccggc gccgatcact gccagcttca tactgtcctc tccggtcgaa
     7921 tcaaaaccat cctcgcttgc aatagctcct aatataacgc ccccgccccc tcccgccatc
     7981 acaaatcggc tgaaaatata cacagtgtgg gaaacgtgaa tgtgattgac aagttgctcc
     8041 ccgcttgtca ggatgcagcc gacaccgtct cgcacacgga tgtcatgatc tgggagaaac
     8101 agaggtccgc ttaccggcgg actcaaggag ggattttcgt ggccgtcgcg gtccgctgcg
     8161 gcggcgaaca ccaacacttc aggagggaga tatgcgctgc atccgcccgc tcatattggg
     8221 cgccgccgtg gcgctgtacg ccggcgcgtc ggccacagcc gaccgcccgc tcggttcagc
     8281 gaaacagttg tcgccgggcg ccgttatcgg cacgtcttat tgggatcggc aatgcaattt
     8341 cgccaccacg cgccgcgtcc acctgagcac cgcggccggg cgcgagctcg tccatcatgt
     8401 gtggaccttt caaccggcgc cgcccgtgtg ggaacggacc gctcactacc gtctgtacga
     8461 catcgcgggc ggcgcctaca gcccgggggt ggacataccc gagggcactc gcatcagggc
     8521 cggcttcggg gatatgtgga tcaccgacga caaccgggcg gtgatcgcgt accaacacta
     8581 ttccgacccg gaccaacgat atcatctgtg gacagccttt gactcggcgg cgggcgccgg
     8641 cgagttctcc aatctggtcg agatccccga ctccatcgac ttctacagct tggtcaacta
     8701 ccaatcgccc ctctggcccc acgtggccta cgtggagggt gtccacgaca cggtccttca
     8761 catcgtggcg caggaatcgc tccagtatcc ggactcggtc gcgatcttct acttccgccg
     8821 cgtcggatcc gattccgacc cgacggcggt gtgggattac ccgcctcgcg tgatcgattc
     8881 caactacaac tggggttccc accagcttgc ggcaacctcc gatggccggg tggctatcgc
     8941 gtggacggcc aacgtgccgt gcgcggggga tcccgacacc gcctcgggag tcggctgctc
     9001 tgatttcgtc ttttgggaga acgatctgta cttccaggtg tcgaacgacc agggtctcac
     9061 gtggcagaat cgggttaacg tgacccgtta ctccgaaacg ccggagcacc cctatcgggc
     9121 gcactgggag atatcggcgc tcttcgatca ccttggcgtc ctgcacatga tatggtcggc
     9181 tgcagcctgg ccgcgaacag cccccgggcc cggtgaggtc gcccagaccg ccagcgccat
     9241 tttccactgg tcggagacca acccgacgcc gaccatggtg cgtgagacaa actggaatca
     9301 gaccgtctgc ggcggcggcg agggccgccc gttgaacatc ggcgacatat cgctcgccga
     9361 gtgcgacgga cgtctctaca ccctgttcgt ccagttcaac gattacgacg ccggcattat
     9421 gagtgactgc tgtgactcga acttgacttt ttaccccgat ggcgccgcta acggcgacct
     9481 gtatatctgt gtctcctcgg acggcggact aacctgggac gctgcccgca atctgacgtc
     9541 cacgcgaact cccggctgca ggttcgacac gcccggcaac gtctgcgcgt gtgaaagctg
     9601 gccctcggtc accccctacg ggagcaatgt ctccatcgac ccgtcaattt ccggggccga
     9661 cgttgttgtc cccgccggcg ggagtgatcc gggttggtat ctcgatgtgc agtacgtgct
     9721 cgaccctttg cccagttcgc cgatgacgca ggaaggttgg cggacggaaa cctccattcg
     9781 atggttccgc atcgcctgtg tcgacccaat ccaggaaccc gatctggcca tattgcccga
     9841 cagcctcgcc tatccggtct ataccccgca ctgcgcggag cgccttgttc cgattgtcct
     9901 ttccaacagc ggtgtcggcg aggggcacta ctcgatcatt gccgaggagt tcgccggccc
     9961 cgccgggtgg ctgggcacga ccggcttctc ggggacagtg gcggccgggg cggccgccga
    10021 taccgggtac gtcgtgctga acccgggcgg ggcgctctgt tctccgggcg ccattgtgcg
    10081 cctggccggc cggctgcgct tcgactcgaa cgatccgaat tctcctcact atgtcctgat
    10141 cgacctcgtg gccgcggaca cggtctgcct gccgtcagcc gacacgctca ccactgcctg
    10201 cgtcgcgctg caggtgttca atgacgcggg gttcggcgga cggggcgccg gcggtgtgac
    10261 gatggactac tcaatgtccg gcgactgcga tccggcggcg gcaacgtact tgtccgacgc
    10321 ctcgcccatc gtcatcgctg acactctcgt caactggtcg gtctatggct gtgatttcct
    10381 tgcgccgcac gggctggtgc ccctcgcgag cgcgtcgggc agcggcggcg gattccgttg
    10441 gagccggtcg ctggccacag tccgcgactc gtcgctcatt ctcgaaaccg actggtacgc
    10501 acccctgcct cccgactcct gcgccttctt aatccagcgg ctcagtctct acgcggctga
    10561 tgctctccct cacacagtct ccttgggcca gctcatcgac tggaatatcc cctcggattc
    10621 gggccttcgc aacggatcgg acacgctggt cgatccggcg attatctatc agtacggcgc
    10681 ggaataccac caggaccccg gcgcctgccg gaacagcgac cgccgcatgg gtgcggtctt
    10741 tccgctggcc atgtatcgcc gcgcggcgtt gcacgagccc cttgtcgcgg actcgagcaa
    10801 cctctggcat tgcgcccaga cccgcgccac cgatgcatgc ctggacaccg cgcggtgcgg
    10861 tctggacgcg gccgaactgg agcttgccct gagcgaaccg ggccgccgcc tttactctgc
    10921 cacggtaccc gaatcgacgt acacggatct ccacaccctc gtcggtttcg tctccgatct
    10981 atcgctggtt ccgggcgaga cgctcgtcgt gtacacaggt ctggcgacgg ttgcggatgg
    11041 cgacacggcg gttctccgcg gcctcgcaga ccaggcccgg cagtggtact gcgaccacct
    11101 cgtgcccgac ccgcccggtt gcggctgttg ccggctccgc ggcgacgtca accgcgacag
    11161 cggcgttaag gtcagcgatc tgactttctt ggcgagctac cttttccggg gaggtccgga
    11221 ggcgctatgc cccgacgaag cggacgccac cggtgacggt gaggtgaagg tttcggatct
    11281 cacctatctg accgcgtacc tgttccgcgg cgggcccgcc cccatcccct gctgagcgcc
    11341 gctccccccg aaatcaaaac gcccgttggg ggcactaccc aacgggcgta tcagaggaaa
    11401 gataagagag ggggattgat gtcttacttc aacaggatca tcttcttcgt gtcgttaaac
    11461 tccccggcct caaggcgata caggtacacg cccgatgcca cccggctgcc gtcggccgcc
    11521 cgaccgtccc attccacctg gacgcgcccg gcatccgacc cgtggtactc gcgcacgagc
    11581 gccccgttga cgttgtagat cgccagcgtc cagtcggccg tgttggccag cgcgaaactg
    11641 atcaccgtgc tcgggttgaa cggattcggg aaattctggt cgagcacaaa ctcgctcggc
    11701 aacaccccgc cggtggccgc caccgagtac gcctgaccct ggtagtcaac cacttcggcg
    11761 cgcgccagga gcagctcgcc cgacccttcg agcgccaccg agaccagctc ctgcggcccc
    11821 gccgggatcg ccgccttacc gatcttgaac agcaggatcc gcagctggcc gtccatcacg
    11881 ttccacttga catccatacc ggcggcggct ccgatcgcgg ccacgttgct gacctgtgcg
    11941 ccgcgcggca cctggtaaac caggagcgcc ccgccgatct cacccggcgc ctccgaactg
    12001 atcgtcaccc gcccctcctc gcgcagcgcg ctcaggatga catcctggcc ccgcggcgcc
    12061 agacgcggga acgggttctc gtcgccgacc agcacccgga tcaggtacac cagatcggcc
    12121 actgtcagcg tcagcccgtc ggcgttcacg tcggtcgcgg cgatctgccc ggcgatgttc
    12181 acggtgaaga ccgacaaccc gtagacaaag tagttggaga acaggaccac gtcgctgatc
    12241 tcgttgggca cattgttcag gttgacgtca ccccggtcgt cgagcgaatc cggatggcag
    12301 atgtggatgc cggcgttata gaattcgacg cagcgagtcg gcgtcggctt gccgggcacc
    12361 ggcacgaagc agtcgtccgg cgcgcccaac cccatgggcc gcgccgcttc cggatagagc
    12421 gcgtcgtcgt cctcgtccca gcgcagaacg tcgttctcga atatccgcag gtcgaccatg
    12481 agattgtagc cggtggggtc ggagaaggcg ttgtcgccgc agtcaaacca gtagaactgg
    12541 acgggcaccc actggtcccc cagcgactgg tcgttggcga cgtagaactc cagggtcgca
    12601 aacaggcccg ccggcgaaag cgccgatgcc ggcggatgct gcgcgccgtt gttcatgtcg
    12661 gcgatcccca ccaggcgcac aaatcgattg gagcagctcg ccccgcagtt gacgtcgtca
    12721 agccggtacg tgaagtactc ccaggcctgg agcgaggccg cgcgggtgag gccggtcagc
    12781 gtcagcaccg acttgtcgaa cgccagcagc agatcgaatc cgccgatcgg cgtcaacgcc
    12841 tcaatgtaga tatccatcgt cttggtcacg ccgaccggag tgcagtcgcc gccgtccagc
    12901 cagatcttca cgcagacatc gcagcccggc ttctccacca cctcgacatc catcgtgtcg
    12961 cgaaccgtgt ggcacccgtc ggtcgcctca aaacagaagc tgtacacgcc gaacgtgtcg
    13021 gggacgaagc acacctggcc gctgtccgtg ccggcaagcg tgaaactgcc cgcgccgcag
    13081 gtccgcgaga gaaccggcgt cgactcggcg tcggttgcga aaatcccgtc gatacagatc
    13141 cggctcgtat cgtcggcgca gcgctcgaaa gtctgcaggc cgggcaccgt gagcaccggc
    13201 gactggtcgg cgcgcacgat cacattcgtg gtcttgctca ccgtgttgca gtagcccacc
    13261 gccgtgagcg taatggggta ggtgccgggc gcgctcaccg gcaggcagac ccgtccggcg
    13321 ctgtacaccg ccgggctcgt caccgtgacg ttcacctgcg tcccggtcac ggtgaccggg
    13381 taacagatcg tggccggacc gctgaaacag accagagtat cctcggtcgc cggcgccgtc
    13441 accgccacct gttccgcgcg cacgatggag acggtgaatt cgcaggtgtc gctcccgcag
    13501 ggcgcagccg cgatcatccg cacgtttatg gtcccggtcg cgctgaccgg aacacatacc
    13561 tgtcccgtgc ccgcgttgta ccacccgacc ggggtcaccg tcaccgtcgc gccggccgga
    13621 ctgaccggag cggccacgca aaccgtgtcg agggcgcaga tcgtatcgac cggcgggctc
    13681 gtcgggcaga tgatcgtcgc cgactgcccg gccaacaccc ggatcgtcac cgtgtccttg
    13741 ccggtggccc cgcaagcgtc ggtcacggtc gcgatcacct ggtatgtgcc cacggccagg
    13801 ttgctgaagc agatcgaatc gggccccacc actcccgcac cggccacgac cgacacgatg
    13861 ttggagtcaa ggtcggcgac ctggtaccgc acgcagacat tggcggtggc gcaggtcgtc
    13921 accggcggcg gggacgtcag cacgatcacc ggcgggcggt ttgtctcgta cgtaatcgtg
    13981 aagctgcagg tatccgcgcc gcagtcgccg gaggcgatca atgtaatcga cttctgtccg
    14041 acgcccgaag gaatcggcac gcagaccgtc ccattgtcta tgaaggcgcc ccccgacgcc
    14101 tggaccgact cgaccgacgc cgatgcgctg tagggcaggc agatcgtgtc gggcgcacac
    14161 aggatgacgg tcgtatccgg cgggcagaga atcgagaccg gttcggcgac gctcacgggg
    14221 acattgaact cgcaggtgtc gccgttgcac ttggccgagg cgatgacgcg cacgctgtag
    14281 acgccgctcg tatcggccgt gaaacagagt tcgttgtttg cccagacgcc gatgtttgtc
    14341 cagaccgagt aaccggcgcc ggtgatctcc agcggcaggc acacctcgcc gggcgcgcag
    14401 agcacaaccg cgggcggggc gccgacgggg cagtcaatgt cgacggaacc gcctgtggtg
    14461 atggtcacgc agaaattcgc cgtatcctgc aggttgcact ggtcggcaat cacgagcgct
    14521 ccgcagtaag taccctccgc gcccggggtg aagcagatct ccgaggctgc ggcgttgtag
    14581 gtaaatccgc cggtcagcga gaccaccgtg accggctggc cgtccgggtc ggcaatcgtg
    14641 acgggcaggc agacctgctg cggctggcac tggctgagag ctgtatccgg ggccgcaacc
    14701 gtcaccgtcg gcggccggtt cgccagcacg gtgatctgca tctggtcgct gtcgctggcg
    14761 ccgcaggaat cgaccgccac cgcggtcacc gtgtacgtgc ccggaccggc gaggtcgacg
    14821 cagatgcggc ttgtccgcgg attgtacacg cctccagtca ccgtaatcga ctgaatgttc
    14881 cgattgacat cgctgatcgc cgccggcagg cagaacgtct cagtgccgca cagggcgagg
    14941 ctggtgtctc ccggcaggaa caccaccggg gcgctgttgc acagcaccgt gacccggaac
    15001 tcccgctggt aggtcgtgca gggcgtccgc accgtcaggc gcacccggtt ctgattggag
    15061 cactgcgacc agaaacagac cgtctgattg gccgcgtccc accaggtgtt gatgccgctc
    15121 accgtcacct gcgccccctc cggcactccg gccacgggga agcagaaggt gtcgaccagc
    15181 gaacacgtga acacggtcgt gtcgcgcggg gtaatgatat tgacgttctc gtccgtcttg
    15241 atggtcacga cggcggtgtc gacgtcccgg ttgccgcact cgtcgattgc cgtgatgatg
    15301 atttcatact ggccgcccgt gtagggcacg aagcacacgc ggccgtcgtt ggtcaccgtg
    15361 ccccggttgg tgatcaccgt gtcgaggtcg ccgtccggat ccgtcaccgc caaggggagg
    15421 cagatccact gggagtagca aagatagatg gtcgtgtccg gaagcggcgc gatcaccggc
    15481 ggctgtccca gatcgaccga aatgttcacc gtgtcgaccg ccgcgccgca ggaatccgtg
    15541 acctgcgcga tgagccggta gagaccggcc gtgtcggcgg ggaagcaaat ccggccggtc
    15601 tcggcgtcgt acgatgcggc gggcgggagg atcgtcagat ctcccgcgga cgcctcgaca
    15661 tcgacacaca cttcgcccgg cccgcagaga ctcaccgccc ggtcgtcgcc gagatccaca
    15721 gagggtcccg atgagaaggc gaccgtcacc cgggtcgtgt cgcggtcgct gaagccgcat
    15781 tcgcccacga ccccgaacac gatcgtgtag acgcccgccg tatccggcgt gaagcagatc
    15841 tcgccggtct cggcgttgaa cgagaacacg tgctccatcg gaagaatgta ggacagcacg
    15901 gtgtccgccg taacactcgc ctcgacacaa acctccgtag ttccgcagac cgagacggcc
    15961 aagtcatctc cgaggctcac gg
//
LOCUS       DAOPOE010000075        15854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4402_length_15854_cov_5.483765, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000075 DAOPOE010000000
VERSION     DAOPOE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4402_length_15854_cov_5.483765"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2287
                     /locus_tag="PKM94_10110"
     CDS             <1..2287
                     /locus_tag="PKM94_10110"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOD67032.1"
                     /translation="SPDIGLMCANYAGYWFIGAALLAVGMLASLLTANSTVAFIVGAV
                     FCSFFVFVNSATWVVSDAVQGFLAPLGVFEHLAEFAGGVISWRAILYFASIVGVMLYL
                     NVILLGRRHWPAEAGGYRFGLHHAVRAASLVAAVIALNALIGSDRVRLDVTAEQLHSL
                     ADQTDELIDQIPDGRPVFIQAFVSPEVPRAYVETRANLLGKLRELDAVGGDKVKVVYH
                     ETEPFSGEAREAREKFGIGPRTVLSTESARTSTQDIFLGVAFTSGPREEVIPFLDRGL
                     PVEYELVRSIRVAANTERKRIGVLHTEANVFGGFDFASMGSLPPWPIVEELKKQYEVV
                     EVDASQPLPKDLDGVLAVLPSALPQAQLDNLKEYVLSGGPTLLLDDPLPVFNIGLSPL
                     LPAEAARNPFTSQGQPTPEPKGDIQTFMKEIGISWNPSQIVWDTYNPHPDLQELQYEV
                     VFLSRNNETAETFNDRYPATAGLQELVMLFGGFLFKAVDSPFEFQELLRTGRQSGVLP
                     YQALVQRGFLGMGFSINRNPRRVATPESYVMAGRVLGSGTVREGDSARAAQVNAIVVA
                     DVDFITEQFFAIRSQGLGTVAFDNVTFFLNCMDMLVGDSSFVALRNKRVAHRTLETVE
                     AQTQEFVRERLAREKEAEEEAQAALAEAQQRLTERVNEVRNRTDLDAQTKQIMAQNLQ
                     EVENRRFEVLKANIEAQKQAQVAAAKEDVEGAIRGIQTRIRTLAVLLPPIPVFAFGVF
                     IFVRRRRREQEGAAAAQRLRS"
     gene            2290..3657
                     /locus_tag="PKM94_10115"
     CDS             2290..3657
                     /locus_tag="PKM94_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOD67033.1"
                     /translation="MTENRKTLVFVGAAAVLTALAFLLAPSTPTPDEFADQGTPFFPD
                     FTDPNTATTLEVVEFDEATGSTKPFKVTFENGRWVIPSHHNYPADAASQLAQTAAGVI
                     EIARDDFRSDNAADYEALGVVDPLGEGAALSGRGKRVTIRGRNGEVLADLIFGKKVEG
                     RDGFRYVRHPEEKRVYAAKVNIDISTRFQDWIDPDLLQLTRSDIERIEVNDYSINERT
                     RSVDRRGQVVLTKGEDGGWKMAGLPSGQTVDSATVAAMLATLDSLKIVGVRPKPEGLS
                     ASLRQDEQQVSMSQADLLSLQGKGFYLSRDGQLMSNEGELLVHTRDGVAYTLRFGEVV
                     YGSGLEVTAGTEDDQSPEPQGNAQNQYLFVTAEFDPKRLPEPKRPSDTSFVGKSDSLL
                     SDADRQQRSLYNAWRAWQGNAERGRKKAQELNTRFADWYYVISHDSFRKLHKSRGDLV
                     AAGKD"
     gene            complement(3718..4776)
                     /locus_tag="PKM94_10120"
     CDS             complement(3718..4776)
                     /locus_tag="PKM94_10120"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD67034.1"
                     /translation="MKGKRVLITGGAGYLGYHLAQNLPRRGVGFLALNDIAPFFKDEY
                     PPDALLVNQDVRDPEGMYRLIRDHRIEVIIHCAAALPLWNRQEIFSTNIGGLRTTLEQ
                     ARKCNIERFIFISSTAVYGVPEKHPLIEDDPLVGVGAYGETKIEGEKICREFRRDGLC
                     ITIIRPKTFIGTARLGVFQILYDWVDSGKRIPIIGRGENLYQLLEVSDLVEAIWLAGA
                     AEPERTNDVFNVGAQHFEKVKTDVGALCEFAGTGARVQPTPAKLVKGTLAFFEFLRIS
                     PLYKWIYGTADKDSYVSTRKIEERLGWKSSFSNQDALISSHRWYLEHKEELKHLKSGV
                     THRVGWNQGVLKLIKKFM"
     gene            complement(5119..7074)
                     /locus_tag="PKM94_10125"
     CDS             complement(5119..7074)
                     /locus_tag="PKM94_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD67035.1"
                     /translation="MKAGLLLVLVAVVVAAAGSALAADPPIPANYPISSPYYELQNEE
                     QIWISPLDSTRVVAVWRDFRLGYRQVAIGIAFGSPDFWVDWLIPPTQQVGEQQSDPTL
                     TVDRNGTFYISVLDYVPAYQFTDSSFISFLKSTDNGMTWSGPVTVEGTSGPYFEDKQF
                     ITVDRTTGPHSGNVYAAWARFPNPTRIMFARSTDGAAGFDPPVIVGQPWDFTDCGWTD
                     VDAGQFANPLVGADGSVYVFWIGVWADPDCNFSYALHCVKSTDGGATFGPPQFIRTTF
                     GNWGQVDGFVDVYNQPVVAADLSAFPHGGNLYVAYANMDTVGSFFDYEIDFLRSLDGG
                     ATWSEPIRINDGLGGDTDSTDQFHPWLVINDEGTLACIWYDQRTDPANHYLFDVYAAY
                     SFDGGASFTRNHRVSEVSINPSSLKKSDRDRPGPDAPAPVAKSALAPMAGLIAEYIGV
                     SIRNNYLHAAWTDTRNGNQDVYGANWEIPFLAPRLISPPDSAVALGPTDDTVALRWST
                     CWRENDDHYTVQIAADAGFSTILAVLDVSAADTVVDAAAFPALSAYHWRVRAHRYALG
                     DATDWSDGRVVRRAATCCVLRGDFNDDGAVKVSDLTALINYLFRGGPAPACAEHGDAN
                     ADGAIKVSDLTLLIDYLFRSGPLPAAC"
     assembly_gap    7097..7106
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7257..10085)
                     /locus_tag="PKM94_10130"
     CDS             complement(7257..10085)
                     /locus_tag="PKM94_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOD67036.1"
                     /translation="MTHVLRSAFLGAALMAWQAVSAAGAHPSYDPDLPQDTVPFYDVS
                     VYDRAVPNPNDFLVEPVGVWPAGWDEMDAYFRKLADASDRVLLEPHGLTHEGRELYHL
                     IISAPENLARLDELRAAMDKVADPEAFGSAAELDAQVRDLPAFCWLAYSIHGDEISGC
                     DAAVRLAWHLAAAGDEATLHLLKNLVILIEPSENPDGRERYLAMLETYRSATPNYDPW
                     SLQHSGVWPRGRGNHYWFDLNRDWILLTQPETEGRVRSIVKYHPVLVIDGHEMGSDGT
                     FLFSPPREPINPNTPANVLKWYEVYGREQAAALDRRGWPYYTGEWNDQWYVGYGSAWP
                     TFFGAVGILYEQAGVDGRFVRQSGDYLLTYHEAVNHQFTSSLANLKTTADHRADILRD
                     YHRSRKEITEAGRAGGLTFLFPPDRDEIKMSRFIRSLVAQGIQVTRATDSFADPSALN
                     AYNEPRGRTAFPAGTFIVSTAQPHGALAKAILEFDPHLSKSFLEEERRELEKHGETRM
                     YETSAWSVPLLYNLDAYFTTGRLPAAVEPITEAAPAGGGVHNADASYGYAVDMVGEKT
                     YLVLNRLFAEDVTVHAAEKPFRLEGRDYGAGALVLRRRGNPDNLPDIVARLAAEVGID
                     IRGISTGYSEIGSKLGAGTFRLLRQPRVALAVGMPLSSGTSGSLWFTIDRQLGMPHSL
                     VSFDRMAWSDLSSYNVLIIPDSWGGLGDLLGGGIPGKIAKWVGDGGTLILIGSSGDWA
                     ADTAGSLSAVRLRSQVLSRLGDYDAALQREIAADAPVVDTMALWHPEKVSAAKEEDKG
                     EREGRATGSDEEADRWARRFHPRGVILQADLNPEHYLAFGLGRRLPVFVWADDCWLAK
                     SPVTAVARLTPDEMKLRLAGLLWPEARRRWAGSAYITHERQGQGQIIMFAGDPTFRAY
                     TYGTRQLLLNAVLYGPGFAGGFESYQQK"
     gene            complement(10188..11306)
                     /locus_tag="PKM94_10135"
     CDS             complement(10188..11306)
                     /locus_tag="PKM94_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOD67037.1"
                     /translation="MMKREYLLGLLFLIFTAVFVYLFYRLIIPFLVPIAWASVFVILF
                     YPLYQRLLRRIHSPGACALIMCLCILLLIIGPLGYLFAMIVSEAADAVAAVNEMYEAG
                     EFDEILRATPPWLEAVKLRLSQYFDISKLNVDELIRDGLDNITGIIFAQTSWLITNAT
                     RAIFHFVLMLFTMFYFFRDGAELVRKVKRLVPLPTEQVDRVTEKLRDVIYATMYGGLI
                     VALIQGTLGGILFAAVGIPSALFWAALMFFLSIIPVFGAFIVYIPAGIILILGGSVVK
                     GLLVIAIGTVVISQSDNIIRPYLVAGRTEMHPLLLFFAIMGGVAMFGLLGLVIGPLIA
                     AVFVALVRLLEIRMHTPEDGQSEADSAGAAPPAPTSRP"
     gene            11789..12094
                     /locus_tag="PKM94_10140"
     CDS             11789..12094
                     /locus_tag="PKM94_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67038.1"
                     /translation="MRRYYRQYETSWIARQLNRTVYSVRYKACSLNIKKANPNVWKGN
                     RGTNNAWNSFAPKTGTRRPTQSRTTRTRRTPRTRQWKATGWHRQNRRSTPRRRNRNW"
     gene            12281..12634
                     /locus_tag="PKM94_10145"
     CDS             12281..12634
                     /locus_tag="PKM94_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD67039.1"
                     /translation="MPVIKYSQVARPPVEGADIDGMTGGPVIGEDQGWKENVLRVFRL
                     APGGHSPRHQHAWEHVNYVIRGRGQLQIGETVHELAEKDFAFVPGEAMHQFSNPYDED
                     FEFICIIPNLNRPPY"
     gene            complement(12806..13435)
                     /locus_tag="PKM94_10150"
     CDS             complement(12806..13435)
                     /locus_tag="PKM94_10150"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD67040.1"
                     /translation="MATTKTAPRLHTVPALGYAFDALEPCIDARTMEIHHDKHHQAYV
                     DKLNAALADHPDLQALAVDDLLRGLGRVPENIRTAVRNHGGGHANHALLWPLLKKGTK
                     FGGPVAEAITQKFGGFAQFKELFGNAAKNQFGSGWAWLVLGPNGLEVTATPNQDSPLV
                     NGATPIVGIDVWEHAYYLKYQNRRPDYVEAFFEVLNWDQANRNYENAKK"
     gene            13638..14048
                     /locus_tag="PKM94_10155"
                     /pseudo
     CDS             13638..14048
                     /locus_tag="PKM94_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
     gene            14523..14744
                     /locus_tag="PKM94_10160"
     CDS             14523..14744
                     /locus_tag="PKM94_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOD67041.1"
                     /translation="MRTFDAIPAALLVIGGLNWGLVGLFHIDLVALVLGDGTLLSRLI
                     YVVVGLSAAYQAMQWKAIQRRRSGKAVAA"
     gene            15026..15098
                     /locus_tag="PKM94_10165"
     tRNA            15026..15098
                     /locus_tag="PKM94_10165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15059..15061,aa:Lys,seq:ctt)
     gene            15190..>15854
                     /locus_tag="PKM94_10170"
     CDS             15190..>15854
                     /locus_tag="PKM94_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD67042.1"
                     /translation="MRTSVMSKADKVEQTWRGTVFEPYLSAFRFDSQVRNLTPKTLDC
                     YLERLGDLFVSLQEQGKSLPEVDKATVQGYALSLKGRVSDETINGRIRVYRRFFGFLV
                     DKGLWESDNPMTGVKLLKTAKKVKPVVDPETVQKIVAGISRDSFEGMRNLLMVLLLWD
                     GMMRKNEMLGLKVSDINLNDHLAKVFGKGRKERMVPLGVKTLKVLHQHLIRWRSKYPG
                     DHL"
BASE COUNT         2898 a         4746 c         5504 g         2696 t
ORIGIN      
        1 cagcccggac atcggcctca tgtgcgcgaa ctacgcgggc tactggttca tcggggcggc
       61 gctgctggcg gtcggcatgc tggcctcgct gctgaccgcc aacagcacgg tggcgttcat
      121 tgtcggcgcc gtcttctgct cgttcttcgt gttcgtgaac tcggcgacct gggtggtgag
      181 cgacgcggtg cagggtttcc tcgcgccgct cggagtgttc gaacacctcg ccgaattcgc
      241 gggcggcgtg atcagctggc gggcgatcct ctatttcgcc tcgatcgtcg gcgtgatgct
      301 ctacctgaat gtcattctgc tgggccggcg gcactggccg gcggaggcgg gcgggtaccg
      361 gttcgggctg caccacgcgg tgcgcgcagc ctcgctggtt gcggccgtga tcgcgctcaa
      421 tgccctgatc ggcagcgacc gggtccgcct ggacgtcact gccgaacagc tccactcgct
      481 ggccgaccag accgacgagc tcattgacca gattcccgac gggcggccgg tgtttatcca
      541 ggccttcgtg tcgccggagg tgccgcgcgc ctacgtcgaa acgcgcgcca acctgctggg
      601 gaaactgcgc gagctcgatg ccgtgggcgg cgacaaggtg aaagtggtgt accacgagac
      661 ggagccgttc tcgggggagg cgcgcgaggc gcgggagaaa ttcggcatcg ggccgcggac
      721 cgtgctgagc acggagagcg cgcgcacctc gacccaggac atattcctcg gcgtggcttt
      781 cacgagcggg ccgcgcgagg aagtcatccc gttcctcgac cgcggcctgc cggtcgagta
      841 cgagctggtg cgctcgatcc gggtggcggc gaacactgag cgcaagcgga tcggcgtcct
      901 ccataccgag gcgaacgtct tcggcggttt cgatttcgcc agcatggggt cgctgccgcc
      961 ctggccgatt gtcgaagaac tcaagaagca gtacgaggtg gtcgaggtcg acgcgtcgca
     1021 gccgctgccg aaggatctcg acggtgtgct cgcggtgctg ccctccgccc tgccgcaggc
     1081 gcagctggac aacctcaagg agtacgtcct ctcgggcggt ccgacgctgc ttctggatga
     1141 cccgctcccg gtgttcaaca tcggcctgtc gccgctgctg ccggccgaag cggcgcgcaa
     1201 cccgtttacg agccaggggc agccgacgcc cgagcccaag ggggacatcc agacgttcat
     1261 gaaagagatc ggaatctcct ggaaccccag ccagatcgtc tgggatacct acaatccgca
     1321 cccggacctg caggagctgc agtacgaggt ggtgttcctg agccgcaaca acgagacggc
     1381 ggaaacgttc aacgaccggt acccggccac cgccggcctg caggaactgg tcatgttgtt
     1441 cggcgggttc ctgttcaaag cggtggactc gccgtttgag ttccaggaac tcctccggac
     1501 cgggcggcag tcgggcgtgt tgccctacca ggcgctcgtg cagcgggggt tcctcggcat
     1561 ggggttcagc atcaatcgca acccgcggcg ggtggccacg ccggagagct acgtcatggc
     1621 cgggcgcgtg ctcgggtcgg ggacagtccg ggagggggac tcggcgcggg ccgcccaggt
     1681 gaacgcgatc gtggtggccg acgtcgactt catcacggag cagttcttcg ccatccgcag
     1741 ccaggggctg gggacggtgg cgttcgacaa cgtgacgttt ttcctcaact gcatggacat
     1801 gctcgtcggc gactcgtcgt tcgtggcgct gcgcaacaag cgggtcgcgc accggacgct
     1861 ggagacggtt gaagcgcaaa cgcaggagtt cgtgcgggag cggctcgccc gcgagaaaga
     1921 agccgaggag gaggcccagg cggcgctcgc cgaggcccag cagcgcctca ccgaacgggt
     1981 caacgaggtg cgcaaccgga ccgatctcga tgcgcagacc aagcagatca tggcgcagaa
     2041 cctgcaggaa gtcgagaacc ggcggttcga ggtgctgaag gccaacatcg aggcgcagaa
     2101 gcaggcgcag gtcgccgccg ccaaggagga cgtcgagggg gcgatccggg gcatccagac
     2161 gcgcatccgg acgctcgcgg tgctgcttcc gccgatcccg gtgttcgcgt tcggggtgtt
     2221 cattttcgtc cggcggcgcc ggcgggaaca ggaaggcgcg gccgccgcgc aacggttgag
     2281 gagctagcca tgacggagaa ccgaaagacg cttgtgtttg tcggcgcggc ggcggtgctc
     2341 acggcgctgg cgtttctgct ggcgccgtcg acgcccaccc ccgacgaatt cgccgaccag
     2401 ggaaccccat tcttccccga tttcaccgac ccgaacacgg ccaccacgct cgaggtcgtg
     2461 gaattcgacg aagcgaccgg gtcgacgaag ccgttcaagg tgacgttcga gaacgggcgg
     2521 tgggtgatcc cctcgcacca caactacccg gccgatgccg cctcgcagct cgcccagacg
     2581 gcggcggggg tgatcgagat tgcgcgcgat gacttccgct ccgacaacgc cgccgactac
     2641 gaggcgctcg gtgtggtcga cccgctcggc gaaggggctg cgctctcggg gcgcggcaag
     2701 cgcgtgacga tccgcggccg gaacggcgag gtgctcgccg acctcatttt cggcaagaaa
     2761 gtcgaggggc gcgacgggtt ccgctacgtc cgccacccgg aggagaaacg ggtttacgcg
     2821 gcgaaagtga atatcgacat ctccacgcgg ttccaggact ggatcgaccc ggacctcctc
     2881 cagctcacga ggagcgatat cgagcggatc gaggtgaacg actactcgat caacgagcgg
     2941 acgcgcagcg tggaccggcg cgggcaggtc gtgctcacga agggcgagga cggggggtgg
     3001 aagatggccg ggctgccgtc cgggcagacg gtggattcgg cgaccgtggc ggccatgctg
     3061 gcgaccctgg actcgctcaa gattgtcggc gtgcggccca aaccggaggg tctgtcggcc
     3121 tcgctccgcc aggacgagca acaggtgtcg atgagccagg ccgacctgct gtcgctccag
     3181 gggaaggggt tctacctctc gcgcgacggg cagctcatgt cgaacgaagg ggagctgctc
     3241 gtgcacacgc gcgacggcgt ggcgtacacg ctccggttcg gcgaggtggt gtacggatcg
     3301 gggctggagg tcacggcggg gaccgaggac gatcagtcgc cggagccgca gggcaacgcc
     3361 cagaaccagt acctgttcgt aaccgcggag ttcgatccaa aacggctccc cgagccgaag
     3421 cggccgtcgg acacctcatt tgtcggcaag tcggacagcc tgctcagcga cgccgaccgg
     3481 cagcagcgga gtctctacaa cgcgtggcgg gcgtggcagg ggaacgcgga gcgcgggcgg
     3541 aagaaagcgc aggaactgaa cacgcgcttc gccgactggt actatgtgat ctcccacgac
     3601 agcttccgga aactgcacaa gtcgcggggg gacctggtcg cggcagggaa ggattgagaa
     3661 gggcgggcgc ccgcagcggc gcccgacccg cggatccggc cggccgcgcc gccccggcta
     3721 catgaacttc tttatcagtt tcagcacgcc ctggttccag ccgacgcggt gggtgacgcc
     3781 cgacttgaga tgcttgagtt cctccttgtg ctcgaggtac cagcggtgcg aggagatgag
     3841 ggcgtcctgg ttggagaagc tcgacttcca gccgagccgc tcttcgattt tccgggtgga
     3901 cacgtaggaa tccttgtcgg cggtcccgta gatccacttg tagagcgggg agatgcggag
     3961 gaactcgaag aaggcgagcg tgcctttgac gagtttggcg ggggtcggct gcacgcgcgc
     4021 gccggtgccg gcgaattcac agagcgcccc gacgtcggtc ttgactttct caaaatgctg
     4081 ggcgccgacg ttgaagacat cgttggtccg ctccggttcg gccgcaccgg ccagccagat
     4141 cgcctcgacg aggtcgctca cttcgagcaa ctggtagagg ttctcgcccc ggccgatgat
     4201 ggggatgcgc ttgccggagt cgacccagtc atagagaatc tgaaacacgc cgagccgggc
     4261 ggtgccgatg aacgtcttgg ggcggataat cgtgatgcac aggccgtccc ggcggaactc
     4321 ccggcagatc ttctcccctt cgattttcgt ctcgccatag gcaccgacgc cgacgagggg
     4381 gtcgtcctcg atcaacgggt gtttctccgg gacgccgtag acggccgtgg aactgatgaa
     4441 aatgaagcgt tcgatgttgc acttgcgggc ctgctcgagc gtggtgcgca ggccgccgat
     4501 gttggtcgag aagatctcct ggcggttcca gagggggagc gcggcggcgc agtggatgat
     4561 cacctcgatg cggtggtcgc gaatgagacg gtacattccc tccggatcgc ggacatcctg
     4621 gttgaccaga agggcgtcgg gagggtattc gtctttgaag aagggggcga tgtcgttgag
     4681 cgcgagaaac cccacgccgc gccgcgggag attctgcgcc aaatgatatc caaggtaacc
     4741 ggcgccgccg gtaatcagta cgcgtttgcc tttcatgttc ccacctgttg gtgtgctccg
     4801 ggatgccgcc ggagtctctc tgttggggtc aggaccaccc cggtattgac ccgctccgca
     4861 ctccgcgcga cgcggcgagt cggatctccc gcccgctttc ggcgggctcg gaggcccgat
     4921 ctccatcaag gtccggcact ctggaacggc gttcgggaca gcgtgtcctg atcggcccgc
     4981 cccgtcgagg gagcgggagg caatatagtc catagatggg agaaggcaag gcggaaatgg
     5041 cgccgccccg tccgggcgct gcgggggggg gcggcgcgta tcgcgcggcg ccgggtgtgt
     5101 tgtccgtccg gcctgttctc aacaggcggc cgggagcggt ccggagcgga acaggtagtc
     5161 gatcaggaga gtcagatccg acaccttgat tgctccgtcg gcgttcgcgt cgccgtgttc
     5221 cgcgcacgcc ggagcgggac cgccgcgaaa caggtagttg atcagggcgg tcaggtccga
     5281 gactttcacg gctccgtcgt cgttgaaatc gccgcggagc acacagcagg tggccgcgcg
     5341 gcgaaccacc cgcccgtccg accaatcggt cgcatcgcca agcgcgtagc gatgcgcccg
     5401 aacgcgccag tggtaggcgg agagcgccgg gaaggcggcg gcgtcgacga cggtgtcggc
     5461 ggcggaaacg tccaggacgg ccaggatggt gctgaagcct gcatcggcgg cgatctgcac
     5521 cgtgtagtgg tcgtcgttct ccctccagca cgtcgaccag cggagagcga cggtatcatc
     5581 ggtcgggccg agtgcgaccg cggaatccgg agggctgatc agccggggag ccaaaaacgg
     5641 aatctcccag ttggcgccgt agacgtcctg gttgccgttg cgcgtgtcgg tccaggcggc
     5701 atggaggtag ttgttccgaa tactgacgcc gatgtattcg gcgatgaggc cggccatggg
     5761 agcgagcgcg gattttgcga ccggggcggg agcgtccgga cccgggcggt cgcggtccga
     5821 ttttttgagc gaactcggat tgatggagac ttccgacacg cggtggttgc gggtgaagga
     5881 tgcgccgccg tcgaacgaat acgccgcata cacatcgaaa aggtagtgat tggccggatc
     5941 ggtgcgctgg tcgtaccaga tacaggcgag cgtgccctcg tcattaatca ccagccacgg
     6001 gtgaaactgg tcagtgctgt cggtgtctcc ccccaggccg tcgttgatgc ggatcggttc
     6061 ggaccaggtt gcgccgccgt cgagcgatcg gaggaagtcg atctcgtagt cgaaaaacga
     6121 tccgacggta tccatgttcg cataggcgac atagaggttg ccgccgtggg ggaaggcgga
     6181 gaggtcggcg gccaccaccg gctggttgta gacgtcgacg aacccgtcga cctggcccca
     6241 gttgccgaag gtggtgcgga taaactgcgg cgggccgaac gtcgcaccgc cgtcggtgga
     6301 cttcacgcaa tggagcgcat aggagaagtt gcagtcgggg tcggcccaca cgccaatcca
     6361 gaacacatag acgctgccgt ccgctcccac cagcgggttg gcaaactgac cggcgtcgac
     6421 atccgtccag ccgcagtcgg tgaaatccca gggctggccg acgatgacgg ggggatcgaa
     6481 accggcggcg ccgtcggtcg agcgcgcgaa catgatccgg gtcgggttgg gaaagcgggc
     6541 ccaggccgcg tagacgtttc cggagtgggg gccggtggtg cgatcgaccg taatgaactg
     6601 cttgtcctcg aaatatggtc ccgacgttcc ctcgacggtc accggtccgc tccacgtcat
     6661 cccgttgtca gtggatttga ggaagctgat gaaggaagag tcggtaaact ggtaggcggg
     6721 cacgtagtcg aggacgctga tatagaacgt gccgttgcgg tccaccgtca gggtggggtc
     6781 ggattgctgt tcgcccacct gctgggtcgg cgggatcagc cagtcgaccc agaagtcggg
     6841 ggagccgaat gcgatgccga tcgcgacctg gcggtagccg aggcggaagt cgcgccagac
     6901 cgccaccact ctcgtgctgt cgagcgggga gatccagatc tgttcctcgt tctgcagctc
     6961 atagtagggc gaggaaatcg ggtagttggc cgggatgggg ggatcggccg cgagtgcgga
     7021 tccggccgcc gccaccacaa ccgccaccaa gaccagcagt aatccagcct tcatagtgat
     7081 cctttcctga gcgggcnnnn nnnnnncgat gacatcggga tagtatatag gactaatata
     7141 gtccggtcta cgtgcggacg caagtcgatt ctgccgcccg cgggtgcggg ggcgtcaggc
     7201 acaagaagcg ggcggagccg taccggcccc gcccgcaaga taccatcgtc gaacggtcac
     7261 ttctgctggt acgactcgaa cccgccggcg aatccggggc cgtagaggac ggcgttcagg
     7321 aggagttgtc gagtgccgta ggtgtaagcc cggaacgtgg ggtcgccggc gaacatgatg
     7381 atctgtccct ggccctggcg ttcgtgagta atgtacgcgc tgccggccca gcggcggcgg
     7441 gcttccggcc agagcagacc ggccaaccgc agtttcatct cgtcgggggt gagccgggcg
     7501 acagcggtga ccggggattt ggccagccag cagtcatcgg cccagacgaa caccggcagg
     7561 cgccggccga gtccgaaggc gaggtagtgt tcgggattga ggtcggcctg caggattacg
     7621 ccgcgcgggt ggaagcggcg cgcccagcgg tcggcttcct cgtccgatcc cgtcgcccgg
     7681 ccctcccgct cgcccttgtc ctcttctttg gcggcggaca ctttctccgg atgccagagc
     7741 gccatcgtgt cgacgaccgg cgcgtcggcg gcgatttccc gctgcagggc ggcatcgtag
     7801 tcgcccagcc tcgacagcac ctggctgcgg aggcgcaccg cggagaggct gccggcggtg
     7861 tcggcggccc agtcgccgct cgagcctatg aggatgagcg tcccgccgtc gcccacccac
     7921 ttcgcgatct tgccgggaat tcctccgccg aggagatcgc cgagtccgcc ccacgaatcg
     7981 ggaatgatga gcacgttgta ggacgacagg tcggaccagg ccatccggtc gaacgacacc
     8041 agcgaatggg gcatccccag ttgcctgtcg atggtgaacc agagtgagcc gctggtgccg
     8101 gagctcagcg gcatgcccac ggcgagcgcc acccggggct ggcggagcag acggaaagtg
     8161 cccgccccaa gcttcgagcc gatctcggag tacccggtgc tgatgccgcg gatgtcaatt
     8221 cccacctcgg ccgcgaggcg ggcgacgatg tcggggaggt tgtccggatt gccgcgccgg
     8281 cgaagcacga gcgctcccgc cccgtaatcg cgcccctcca ggcgaaacgg tttctcggcc
     8341 gcgtggacgg tcacatcttc agcaaacagg cgattgagca caaggtaggt cttctcgccg
     8401 accatgtcga cggcgtagcc gtaggaggcg tcggcattgt ggaccccccc gccggcgggc
     8461 gcggcctcgg tgatcggctc gaccgctgcc gggagacggc cggtcgtgaa gtaggcgtcc
     8521 agattgtaca ggagaggcac cgaccaggcc gaggtctcgt acatgcgggt ttcgccgtgc
     8581 ttctccagtt ccctccgctc ctcctcgagg aacgacttgc tcagatgcgg gtcgaactcg
     8641 aggatggctt tggccagggc gccgtggggc tgggcggtgc tcacgatgaa agtgccggct
     8701 gggaacgcgg tgcggccgcg cggctcattg tacgcattca gcgccgacgg gtccgcgaaa
     8761 gaatcagtcg cgcgcgtcac ttgaatgccc tgggcgacga gggagcggat aaagcggctc
     8821 atcttgattt cgtcccggtc gggggggaag aggaacgtga gcccgccggc gcggccggcc
     8881 tcggtgatct ctttccggga gcggtggtag tcgcgcagga tatcggcgcg gtggtcggcg
     8941 gtcgttttca ggttggcgag cgacgaggtg aactggtggt tgaccgcctc gtggtaggtc
     9001 agcaggtagt cgccgctctg gcggacgaac cggccatcga cgcccgcctg ctcgtagagg
     9061 attccgaccg cgccgaaaaa cgtcggccag gcggagccgt agccgacgta ccactggtcg
     9121 ttccactcgc ccgtatagta gggccagccg cggcggtcga gcgccgcggc ctgctcgcgg
     9181 ccgtacacct cgtaccactt cagcacgttc gcgggagtgt tggggttgat cggctcgcgc
     9241 ggcggcgaaa acagaaaggt gccatctgag cccatctcgt gcccgtcgat tacgagcacc
     9301 gggtggtatt tgacgatgct tcgcacgcgg ccttcggtct ccggctgggt caggaggatc
     9361 cagtcgcggt tcaggtcgaa ccagtagtgg ttgccccggc cgcgcggcca gacgccggag
     9421 tgctggagtg accagggatc gtagttgggc gtggcgctgc ggtaggtctc cagcatcgcg
     9481 agatagcgct cccggccgtc gggattctcc gacggttcga tcaggatgac caggtttttg
     9541 agcaggtgga gcgtcgcttc gtcgccggcc gccgccagat gccacgccag gcgcaccgcg
     9601 gcatcgcagc cggagatttc gtcgccgtgg atgctgtagg cgagccagca gaaagccggg
     9661 aggtcgcgca cctgcgcgtc gagttcggcg gccgagccga acgcctccgg gtcggcgacc
     9721 ttgtccatcg ccgcgcggag ctcatcgaga cgggcgaggt tctcgggagc cgagatgatc
     9781 aggtggtaga gctcgcgccc ctcgtgcgtg aggccgtggg gttccagcag aacgcggtcg
     9841 gaggcgtcgg ccagtttcct aaaatacgcg tccatctcgt cccaaccggc cggccagacg
     9901 ccgaccggct cgactaggaa gtcgttcggg ttgggcacgg cccggtcata cacgctgacg
     9961 tcatagaagg ggacggtgtc ctgcggcaga tcggggtcgt aggacgggtg ggcgccggcg
    10021 gccgacacgg cctgccacgc catgagagcc gccccgagga aggctgagcg aagcacgtga
    10081 gtcatacgcg atctcctagt gcgcgaaggt ggcttgtcca ttcgggataa gaaaccggcg
    10141 gggccagggg gtgtaaaggg attttctcgc cgcgcggcgg cggcgcctta cggccgagaa
    10201 gtcggggcag ggggagccgc gcccgcgctg tcggcctcgg actgcccgtc ctcgggcgtg
    10261 tgcatgcgga tctcgagaag ccggacgagg gcgacaaaaa ccgcggcgat gagcggcccg
    10321 atcaccagtc ccagcaggcc gaacatcgcc accccgccca tgatggcgaa aaacagcagc
    10381 agcggatgca tctcggtgcg gccggccacg aggtaggggc gaatgatgtt gtcgctctgc
    10441 gatatcacca ccgtgccgat cgcgatgacg agcaggccct tgacgaccga gccaccgaga
    10501 atgagaatga tgccggcggg gatatacacg atgaaggccc cgaaaaccgg gatgatcgac
    10561 agaaagaaca tgagagcggc ccagaaaagg gccgagggaa tgccgaccgc ggcgaacagg
    10621 atccccccga gcgtcccctg gatgagggcg acgatcagcc cgccgtacat cgtcgcatag
    10681 atcacgtcgc gcagcttctc ggtgacccgg tcgacctgct cggtcggcag cggcaccagg
    10741 cgcttcacct tgcggacgag ctccgcgccg tcgcggaaaa agtagaacat cgtgaagagc
    10801 atgaggacga agtgaaagat ggcgcgcgtg gcgttggtaa tgagccagga ggtttgcgca
    10861 aatatgatac ccgtgatatt gtccaacccg tcgcgaatca actcgtcgac gttgagcttt
    10921 gaaatgtcga agtactggga gaggcggagc ttgacagcct ccagccaggg gggagtggcc
    10981 ctgaggattt catcgaattc ccccgcctcg tacatctcgt tcacggccgc caccgcgtcg
    11041 gccgcctcgc tcacaatcat cgcaaacaga tagccgaggg ggccgatgat aagcaggagg
    11101 atgcacaggc acatgatgag cgcgcaggcg ccgggagagt ggatgcggcg caggagccgc
    11161 tggtagagcg ggtagaagag gatcacgaac acgctcgccc aggcgatcgg gaccaagaag
    11221 gggatgatga gccggtagaa aaggtagacg aagacggcgg tgaagatcag aaagagcagg
    11281 ccgagcaggt attcccgttt catcattcgc tatccgattg gcgtgagtat ctccggagca
    11341 gtttacggca attgcgggtg gggagcaaag taaaaagcgg ggcggggggt tcgcggaggt
    11401 tggcggcggc tacgcaagtt gctgctgtgc aatatcttgt ctcatcgcta ggcgggcgag
    11461 gaagtgatcc gggagaattg atctgcagcg gggcgccggc tgtggccgca tcggccggag
    11521 atggacgggg gttctctttt ttcggttcct cgcgaaaaga taagcgcttg gggtcaagga
    11581 ttttaggaat aggtcgaaaa ttaaataaag aaaaaccgac ggcgggcatt gagtgtctcg
    11641 ctcggtgctc gtgaatttaa acgcattcat taagaaggag tctggtatgg ctacgcgtag
    11701 aaccaacact gcccgtcgtg ctgtgccgcg tcgtcgcgct tatcagcgca aggttacccg
    11761 tccgtggtct cgtcaggaga tccaattcat gcggcgctac taccgccagt acgagacctc
    11821 atggatcgcc cgtcagctga atcggactgt ctactcggtt cgctacaaag cctgcagcct
    11881 gaacatcaag aaggcgaacc cgaacgtgtg gaaggggaac cggggcacca acaacgcgtg
    11941 gaattccttc gcgccgaaga ccggtacccg ccgtccgacc cagagccgga ccacgcggac
    12001 ccgccgcacg ccgcggaccc ggcagtggaa agcgaccggc tggcatcgcc agaaccggcg
    12061 ttccaccccg cgccgccgca accgcaactg gtaaggacgc cgacggcgct gccgaacaga
    12121 atacgacgta cctttcatag atgacccgcg ggcgctgctc cgcgggtcat tttttttgga
    12181 ggggaggctt cccccgcccg gccgcggcac gggatcttcc cgcgggattg cccgccgccg
    12241 ggcgacatgc tatattgccg gacagaagga gattcttgtt atgccggtaa tcaaatacag
    12301 ccaggtcgcg cgcccgccgg tcgagggggc cgacatcgac gggatgaccg ggggaccggt
    12361 gatcggcgag gaccaggggt ggaaagaaaa tgtcctgcga gtgttccgac tggcgccggg
    12421 aggacactca ccgcgccacc agcatgcctg ggagcacgtc aactacgtta tccggggacg
    12481 ggggcagctc cagatcggcg agactgtcca cgagctggcc gagaaagact tcgccttcgt
    12541 gccgggggaa gcgatgcacc agttctcgaa tccgtatgac gaggattttg agttcatctg
    12601 catcattccg aatctgaacc ggccgccgta ttagccggga gggaaggggg gtctgcaagc
    12661 aaccgcggcc gccggcggtg aagccggcgg ccgcgcgtcg tcacgaggta cagcacggga
    12721 aatcagggat atcgcccggg cgcattcatt ctgcaaaccc ggccgcacgg tcggatgccc
    12781 cgggccttcg gccctcgacc ccaacctact tcttcgcgtt ctcgtagttc ctgtttgcct
    12841 ggtcccagtt gaggacttcg aaaaacgcct ccacgtagtc ggggcggcgg ttctggtact
    12901 tcaggtaata ggcatgctcc cacacatcga tccccacgat cggcgtcgcg ccgttcacga
    12961 gggggctgtc ctggttggga gtggcggtaa cctcaaggcc gttgggaccg agcacaagcc
    13021 aggcccagcc ggagccgaac tggttcttgg cggcgttgcc gaagagctcc ttgaactgcg
    13081 cgaagccgcc gaacttctgc gtgatcgcct cggcgaccgg accgccgaat ttcgtcccct
    13141 tcttcagcag cggccacaag agggcgtggt tggcgtggcc gccgccgtgg ttgcgaaccg
    13201 cggtgcggat attctccgga acgcggccga gaccgcgcag gagatcgtcg accgccagcg
    13261 cctgaaggtc cggatggtcg gcgagcgcgg cgttgagctt gtcgacatag gcctggtggt
    13321 gcttgtcgtg gtggatttcc atcgtcctgg cgtcgatgca gggttcgagg gcatcgaagg
    13381 cgtacccgag ggcggggacg gtgtgcaggc ggggggcggt ttttgtggtt gccatacgaa
    13441 caaacctccg ttaaattgca ttaattggag ctataaagtc tccgtttcct tcgtagtact
    13501 atggtaacac gcgacgcgcc cgccggttcc cgccggccgc cgcgaaccgg ccgtccgcag
    13561 ccccgcaccg ggcggggcgc ggcgccgagg cgcgggaaca caattgagga gatgagtcga
    13621 tggaagctat ggagtcgatg tatgaccgcc gcctggtggc cccgatgtgg caggagctgc
    13681 aggagatcgg cgtgcagccg ctgaccacac cggaggcggt ggaggccgcg atggaggaga
    13741 cggccgggac gaccctggtc ctggtcaatt cggtgtgcgg gtgcgccgcc ggcagcgccc
    13801 gcccaggcct ggccctcgcg ctgcaaaacg agcggatccc ggaccggctc tacacagtct
    13861 tcgccggggt cgaccgggcc gccaccgaac gggcgcgcac cttcatgccc ggcgtgccgc
    13921 gctcgtcacc ttcggtcgct ctgttccggg acggcaagct ggtgtttgtg ctgccgcggc
    13981 gggcaatcga ggggaaaacc gagagcgaag tggcccaact gctgatcccg gcgttcaatg
    14041 aacactgcac gcggaaaggg ccctcggttc cgcgcgaaca gatgctggcg gtgtatcggc
    14101 agtatgagcc tccctgcagc tcagagttgc gggtgaaata gcagttcaaa acaacacggt
    14161 cgagtgagga caggtcgggc aggtgtttga gctcctgccc gttcttcttg gcagggtacg
    14221 gaatccgggt ctcgacgatc aattatatta gacaaacact caatgtgatg gggtgggtcg
    14281 ttacgattgt actctgtacg gggagccgtc cgtcacgcca agaacaatgt tgtacattgt
    14341 tatacagcca tttacgcgaa aagcatcggt tgccgtcgcg acccggaact ctcacgtggg
    14401 attatcggtt atattttaca acgattagac aataatgcaa cagaatagca caacgccaag
    14461 gtcgaagcaa gccattgccg gtacgctgag acaagggaca cgaaaaggag aagacgaaga
    14521 ccatgaggac atttgacgcc atccctgccg ctctgctggt catcggcggg ctcaactggg
    14581 gcctggtcgg gctgttccat atcgacttag tcgcgctggt gctcggcgac gggaccctgc
    14641 tcagtcgcct gatctatgtc gtcgtcggac tcagcgccgc ctaccaagcg atgcagtgga
    14701 aagcgattca gcggcgcagg agcgggaaag cggtcgctgc ctgaacccct ctgttgttgt
    14761 gttcccatcc ggtccgcccg gtccttcggg accgggccgg gccagtcaaa cgctatcggg
    14821 agacggtgcg cgattgggca ggcggtgttc gaagatatgg ggacgcgccg ggcggcagcg
    14881 ggggcggggc gttccgagcg gggggcgcaa aaagaccttg atttcgccgg ggtgggccgt
    14941 tattctcgtt gttgcctggc cttggggccg ggcgctgtgc gacaatgaga cggaaaccac
    15001 cgggtttccc cgaaagctga aaattgcgcc attagctcag ttggtagagc agctgactct
    15061 taatcagcgg gtccggggtt cgaatccctg atggcgcatt cttctttggg cacagcaatc
    15121 ggttacgact cctaatcaca ctgactgctg acctcagaga ggccaaaagg gcctctgatc
    15181 ggaggagtca tgcgaaccag tgtgatgagc aaggccgaca aggttgaaca aacctggcgg
    15241 ggtaccgtat tcgagccgta tctgtcagcc ttccggttcg acagccaggt cagaaacctc
    15301 acccccaaaa cgctcgactg ctaccttgag cggttgggcg acctgttcgt gagcctccaa
    15361 gagcagggca aatcgctccc cgaagtcgac aaggcgacgg ttcagggcta cgcgctttcg
    15421 ctcaaggggc gtgtatccga cgagaccatc aacggccgga ttcgggtcta tcgccggttc
    15481 ttcggcttcc tcgtcgataa agggctatgg gagtccgaca acccgatgac cggcgtcaag
    15541 ctcctcaaga ccgctaagaa ggtcaagccg gtggttgatc ccgaaaccgt gcagaagatc
    15601 gtcgccggca tcagccggga cagctttgag gggatgcgca acctcctcat ggtgctcctg
    15661 ctgtgggacg gtatgatgcg caagaatgaa atgctcgggc tgaaggtctc cgacatcaac
    15721 ctcaatgacc atctggccaa ggtgtttggc aagggtcgga aggagcgcat ggtgccgctc
    15781 ggcgtcaaga ccctcaaggt cctccaccag cacctcatca ggtggcggtc gaagtatccc
    15841 ggcgaccatt tgat
//
LOCUS       DAOPOE010000076        15323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4600_length_15323_cov_5.294931, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000076 DAOPOE010000000
VERSION     DAOPOE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4600_length_15323_cov_5.294931"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PKM94_10175"
     CDS             complement(<1..1395)
                     /locus_tag="PKM94_10175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67043.1"
                     /translation="MNANLDIIVIPFHDWKKVQREGGRTRDAHLMSHLARHPLVNRML
                     VVNRPVSVPEVMVRPGRFRTAGERIGGGLGQRLTRVANSVFVLDTLLPDLFAPVRQRR
                     GWFLDAFAGATVAQSVQWAASRLGIGAFHLVSFNAYAGNLAARLSPVRSLFDAWDNFL
                     KFPANAQFRKRLEQSYRMYAECTDVWSTNSATNREVFCRLFEPGACEVIRNGVDPDHF
                     RTARAIPADLAALPRPIIGLGAKITHLVDYDLLNAVMAANPDLSFVVIGQVLDKAVMG
                     RIKPLPNFHYLGDKHYDVYPAYVGGFDAAIVPYVVGAREHGGDSIKTYEYLAAGKPVV
                     STPIDGLLKCPGRIFPARSPEEFGAAIRKALSAGDQRVEIPAEYLWSTKAEEMMDLLV
                     RPRRPRTRRTHRPIVAFCGTRGVPARYGGFETAVDQISRRFAARGYDCEIICRRSAAG
                     PPAGVGSRPLPDEHE"
     gene            complement(1392..2612)
                     /locus_tag="PKM94_10180"
     CDS             complement(1392..2612)
                     /locus_tag="PKM94_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD67044.1"
                     /translation="MGFALYANLARVALGSRYVLLVADSLLMVLVLYTASLRLLAGQL
                     PTAVEAAVLALLGLGAAAIMHPNIPDFRTGIEGFRQTLYQMSAVSVGVALLGTRRRMM
                     HFLSIVAVLAVPILLWAVKQFIAISALDLAIIGANTADIYTWQIFGKVRAFGFFSGPF
                     HMGLFAGFVFWIGAALYFESRRRIWLVLAALSFLACLATLTRGSLIAMCGSVPVVLFF
                     VFPRARLRVAAATAVVLAGIGLGLYLLSSHVDAVGSVVESVLTLSELSDDSRLVGRFD
                     GYREGIAAVATHPFGIGMGSAADALGHYFDPYKYVHITSHNMFLRMALETGWMGLGLF
                     MAILWLALRAARRTARMGDHPMAMLLMGWPAIVLITGITGSSINAFPVNLLFWLIVGG
                     AVRLTAHSGPGATT"
     gene            complement(2698..3741)
                     /locus_tag="PKM94_10185"
     CDS             complement(2698..3741)
                     /locus_tag="PKM94_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67045.1"
                     /translation="MARLCLETAIGQLTVCAVTVTYGDRWHLLRRVLERLCAWPAVAR
                     VIIVDNGATYDLRGRTAGAFGLRVEVVGSGRNEGSARGYAIGMKAAREAVDTEFVWLL
                     DDDNFPESGALEALAEAWRELSAGQASDRLALLALRTDRPYLVQIAAGADPRRIFPKA
                     NSFLDFHAADAPRRVWRKLFRRRPALTTVKSRSVKIPYGVYGGLLFHKTLLDRVGYPD
                     ESYFLYGDDYDFTTRISDGGAVYLVAGSRIDDLDPSWHVSSTARRVTVFQNLSQAPDF
                     RVYCTVRSNVIFVRRHLLTRPWVWQLNRLAYLAILRAFMWATGRRDRYDLIARAVSDG
                     CSGRLEKVGCHVW"
     gene            complement(3722..5023)
                     /locus_tag="PKM94_10190"
     CDS             complement(3722..5023)
                     /locus_tag="PKM94_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67046.1"
                     /translation="MLKTGLLNSLLRSLTLGSKFLLLLFLARVVTPAELGVYGLVVVT
                     ITVTLYLLGLDFHTYNTREILGAAPPDRPCFVRDQMAFHGLVYAAVLPLTLAVFISGT
                     LPWRCAGWFYLLLVLEHLSQEAYRLLVTFSRSSLANLVLFLRAGVWVYAVVGVGLAAP
                     GAVTLGRVFCAWAVGAAASIALAAATLRRLPWREALEKPVDWNWIRRGVGVSLRLFAA
                     TVCLLGVQYADRYFLQHFHGPAAVGVYTFFAQIANTVQIFVYTAVVIVVLPRLVGAWQ
                     RGDLALYRCHMRRLALGVLGVIALVSVAAAACFGPALAMVGREVYREQLPAGWVLLAA
                     MALMGAAYIPHLALYARGRDSAIVAASAAACVAGLVLHALLVPAYGPIGAALGTLAAA
                     GVLGGAKLVFLMRNAEGPARTAGQTMTADSTREACRGAIVS"
     gene            complement(5292..5762)
                     /locus_tag="PKM94_10195"
     CDS             complement(5292..5762)
                     /locus_tag="PKM94_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOD67047.1"
                     /translation="MRCDIEGVIIRELRCVADARGWLTELFRRDELPGAFEPAMSYVS
                     MTRPGVVRGPHEHRDQSDLFCFLGPSTFRLYLWDSRPGAPSHGRTYRAEFGEHRRALV
                     IVPPGVVHAYKNVGAVDGLVFNAPNRLYAGEGRREPVDEIRHENDPASPFRIDD"
     gene            complement(5752..6648)
                     /gene="rfbD"
                     /locus_tag="PKM94_10200"
     CDS             complement(5752..6648)
                     /gene="rfbD"
                     /locus_tag="PKM94_10200"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOD67048.1"
                     /translation="MRDRLLVTGARGQLGTDLVAEFRRRADVVGCDLDDFDITDPAAV
                     RQHVAAVRPTVVIHAAAFTEVDRCEAEPERAIRVNADGAAFVAEACRAVGAHLVFYST
                     DYVFDGARRTPYVESAPPNPRTVYGRSKLEGERRTSAAQPDSAILRLAWMYGAHGRNF
                     VVTMLARGAEQLEAARRGESIASLQVVDDQVGNPTWTRDVVRQTEIVLRERLTGLYHC
                     SAEGETSWYGLAAFIFSRLGMAVDLRPCTTAALGRPAPRPAYSSLENAALKAAGCHVM
                     RDWREALAEFLAGRREAALYAV"
     gene            complement(6638..7528)
                     /gene="rfbA"
                     /locus_tag="PKM94_10205"
     CDS             complement(6638..7528)
                     /gene="rfbA"
                     /locus_tag="PKM94_10205"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOD67049.1"
                     /translation="MSAGIRKGIILAGGAGTRLHPVTQVACKQLLPIYDKPMIYYPLS
                     TLMLFGVTEILIISTPQDLPRFRDLLGDGRAVGLTLRYAVQERPEGIAQAFLIGADFI
                     GRDNVALILGDNVFYGVYDFLRGVRAFVDGAVVFGYYVKDPQRYGVVEFDHDGNAISI
                     EEKPAAPKSNYAVTGLYLYDAEVVSVARSLRPSARGELEITDVNNHYLRRGRLKVVRL
                     GRGIAWLDTGTHESLLDASNFIATIEKRQGQKIACIEEIAYRMRYINRRQMEALLASI
                     PDTAYAQYLRDVVREVNGEG"
     gene            complement(7541..8608)
                     /gene="rfbB"
                     /locus_tag="PKM94_10210"
     CDS             complement(7541..8608)
                     /gene="rfbB"
                     /locus_tag="PKM94_10210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOD67050.1"
                     /translation="MGDETFQRTVVVTGGAGFVGSNLLLHLVPAYPDCRWVNVDCLTY
                     AGNLANLKAIEKAPNYRFARIDITDFDRLADCFHAFDPSGIIHLAAESHVDRSILGPS
                     TFIRTNILGTFNLLELARKKTQRSGPQAIRFHQVSTDEVFGSLGPEGHFTEDTPYRPN
                     SPYSASKAAADHLVRSYAATYGLDTVTTHCSNNYGPYQFPEKLIPLMIRNARAGLPLP
                     VYGDGKNVRDWLYVEDHCRALDLVFHEGKSGATYTIGGHNEIENLELVRLICRMLDDR
                     LGGGPRENLITFVTDRLGHDRRYAIDASKIARELGWRPSVTFAEGLCRTIAWYLDHQE
                     WLDHCISGQYLQYYEDIYAGR"
     gene            complement(8705..8777)
                     /locus_tag="PKM94_10215"
     tRNA            complement(8705..8777)
                     /locus_tag="PKM94_10215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8742..8744),aa:Phe,seq:gaa)
     gene            complement(8840..9214)
                     /locus_tag="PKM94_10220"
     CDS             complement(8840..9214)
                     /locus_tag="PKM94_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67051.1"
                     /translation="MTEKSDTRPVPFKLAYWWAFGFATVFLLYGLVTLVLSFLDRQYA
                     NLAQPFGFLLIGGVLMAAAWGLRNRRSWAWYGEIGINGLIIIAALAGFRNYANLIILA
                     LAAVALALLLTSATKRYLFPGR"
     gene            complement(9227..10198)
                     /locus_tag="PKM94_10225"
     CDS             complement(9227..10198)
                     /locus_tag="PKM94_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOD67052.1"
                     /translation="MAAGQIFAGIDIGGTTIKYGLFDEQGKVQFREQKPTMVEKGPKP
                     LMHLAANIGERLLYTAAEEDYEVRHLGVGTPGAVDFHTGRVIGPCPNIPGWQGTEIGA
                     GLRERLNVPVWVDNDVNAMALAEAHFGAAHGAESALCLTVGTGVGGAVIFGGRIWRGS
                     SWSAGEVGHMTINFDAPFTHGGMPGSIEGYCSSQAILGRLRRELEREMTPGFEAVLEG
                     NLDNLSIRKIFAALRKDDAAARKAITEAATYLGIGLAGVVNLLNPEMIVIGGGIAEGG
                     GGFVEIVAAKIRELAFDSAVQNLKVVKAALGNDAGFIGAGLLGEFAA"
     gene            complement(10388..10729)
                     /locus_tag="PKM94_10230"
     CDS             complement(10388..10729)
                     /locus_tag="PKM94_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67053.1"
                     /translation="MDNGCAHPHHLLEEFSPAEARAIEVHKYFLSLEAGTDVGLEYAV
                     RHWLIHHSTTWRQARLQEELDAQRSEIQKHKWIESERAGHDLGQGAVEDWISRYAAQW
                     RHWREQQEHQS"
     gene            10922..12217
                     /locus_tag="PKM94_10235"
     CDS             10922..12217
                     /locus_tag="PKM94_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD67054.1"
                     /translation="MADAHDEHRASRWSLPARYGANREVVALSVARLAEALGNSILTI
                     VIPLYVAEIAHPLFNLPVPVLVGTLISLYGIVSAAVQPVMGALSDRLGRRKVQIQAGM
                     ALMGLGTLAFMAATSYSHLLVLRAIQGIGVATTIPASMAVMATATRRHTRASSMGIYS
                     TLRLIGFAGGPVIGGFLKVRFGFDAAFIVGAAFVLLAMILVQVWVRETPPAEPAAPPA
                     PGRLRILDRDLLDPGILAAALATLLMAANFSMVTTLENEFNARLDMTAFEFGIAFAMV
                     MVSRLVFQVPVGRLADTIGRKPLMLGGLLVLAPATGLLGYVTSPAQLMAVRFLQGCGG
                     AGIAAPAFAVAGDLAREGGEGRQMSLVTTGFGLGMTIGPLIAGPLSLFFFQLPFLVGG
                     ALALAGLWAVYRYMPETVAGGRGWFRSAAPEKVSDRNAG"
     gene            12336..12698
                     /locus_tag="PKM94_10240"
     CDS             12336..12698
                     /locus_tag="PKM94_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD67055.1"
                     /translation="MNDRRDNARRRTAAYHLVYELETGEFVGRVINLTTGGLRLISDA
                     AVAVPTKLSCRMRLPDLFKGAREIMFDIESRWCEYNRIGDWYETGYEFVRLGELARQA
                     IDAMIRDWPRETTPAKTR"
     gene            complement(12724..13176)
                     /locus_tag="PKM94_10245"
     CDS             complement(12724..13176)
                     /locus_tag="PKM94_10245"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOD67056.1"
                     /translation="MQRLNPIRVAYMRHIGPYHEVGPTFTRLCQWAGERGLSRSGLRL
                     IGASWDNPAVVPAALLRYDACIEVEEDFHPVDEIPVQTFPGGEYAVYRHVGPYSGLAE
                     AFRRLLYEWLPSSGRHSRGGACLEIYAPQDHAVTPPAELITDLLIPVR"
     gene            complement(13340..13831)
                     /locus_tag="PKM94_10250"
     CDS             complement(13340..13831)
                     /locus_tag="PKM94_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOD67057.1"
                     /translation="MAQAKRVPTGQERAIPHLVVDNAAAAIDFYRKAFGAEELMRMPA
                     PDGRRLFHAEIRLGASVIYLCDDFPEMCGGRSQTPKALGGTPVTIHHYVEDCDAIIQR
                     AGKAGAKVVRPPEDMFWGDRYGAVEDPFGHHWSFASHVRDVSPQEMAAAVEAMARETA
                     AKS"
     gene            complement(14038..14154)
                     /gene="rrf"
                     /locus_tag="PKM94_10255"
     rRNA            complement(14038..14154)
                     /gene="rrf"
                     /locus_tag="PKM94_10255"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(14343..>15323)
                     /locus_tag="PKM94_10260"
     rRNA            complement(14343..>15323)
                     /locus_tag="PKM94_10260"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2781 a         5141 c         4850 g         2551 t
ORIGIN      
        1 ctcgtgctcg tccggcagag ggcggctccc cacgccggcc ggcggcccgg ccgcggagcg
       61 ccggcagatg atctcgcagt cgtagcctcg ggcggcgaag cggcgggaga tctggtcgac
      121 ggcggtttcg aagccgccgt agcgggcggg caccccgcgc gtcccgcaaa aagcgacgat
      181 gggacgatga gtgcggcggg ttcggggccg ccgcggccgc accaggagat ccatcatctc
      241 ttctgccttt gtgctccaca gatactctgc agggatctcg acgcgctggt cgcctgcact
      301 caaggccttg cggattgccg ccccaaactc ctcaggcgac cgcgccggaa aaatgcggcc
      361 ggggcactta aggagtccgt ctattggtgt tgatacgacc ggctttccgg cggcgaggta
      421 ctcgtatgtt ttgatggaat cgccgccgtg ttcacgcgcc cccaccacat acgggacgat
      481 cgctgcatcg aacccgccga cgtaggccgg atagacgtca tagtgcttgt cccccaggta
      541 gtgaaaattc ggaagcggct tgatgcggcc catcaccgcc ttatcgagaa cctgcccgat
      601 gacgacgaac gacaggtctg ggttggccgc catgacggcg ttgagcaagt cgtaatccac
      661 gaggtgagtg atcttggctc ccaggccgat gattggccgg gggagggcgg cgagatcggc
      721 cgggatcgcg cgggcggttc ggaaatgatc cgggtccacg ccgttgcgga tgacttcgca
      781 tgctcccggc tcaaataggc ggcaaaagac ttctcggttc gtggccgagt tggttgacca
      841 gacatcggtg cattctgcat acatgcggta gctttgctca agccgtttgc ggaattgggc
      901 atttgcggga aacttgagga agttgtccca cgcgtcgaag agcgaccgaa cgggcgatag
      961 gcgcgcggcc aggttcccgg cgtaggcgtt gaagctgacg aggtgaaagg cgccgatacc
     1021 cagtctcgac gccgcccatt gtaccgactg cgccacagtc gcgcccgcga aggcatcgag
     1081 gaaccaccca cgccgctgcc gcaccggcgc aaagagatcg ggcaacagag tgtcgaggac
     1141 gaacacgctg ttcgccactc tggtcagacg ctgccccaat ccgccgccga tccgctctcc
     1201 cgccgtacgg aagcgaccgg gtcgaaccat cacttccggc acacttaccg gccgattgac
     1261 gaccaacatt cggttgacaa gcgggtgccg agccagatgc gacatcagat gcgcgtcgcg
     1321 ggtgcggccg ccttcgcgct ggaccttctt ccagtcatga aaaggaatta cgatgatgtc
     1381 gaggttggcg ttcatgttgt tgcccccggt ccggagtgag ccgtcagtct cactgcgccc
     1441 ccaacgatca gccagaacag caagttcacc ggaaaggcgt tgattgagga tccggtgatt
     1501 cctgtaatca gtacaatggc cggccatccc atcagaagca tcgccatcgg atgatcgccc
     1561 attcgcgccg tgcggcgcgc cgcgcggagc gccagccaca gaattgccat gaaaagcccc
     1621 agtcccatcc agccggtttc cagcgccatg cgcagaaaca tgttgtggct cgtgatgtgg
     1681 acgtatttgt acgggtcgaa atagtggccc agggcatcgg cggcgctccc catcccaatc
     1741 ccgaacgggt gggtcgcaac cgctgcgatt ccctcgcggt agccgtcgaa tcgacccacc
     1801 agccggctgt cgtccgacag ttcactcagc gtcaacacgc tctcgactac actcccgacg
     1861 gcatccacat gagaggataa caggtagagc ccgaggccga taccggccag cacaaccgcc
     1921 gtggccgccg ctacccgcag gcgcgcgcgc ggaaagacaa agaacagcac caccgggacc
     1981 gagccgcaca tcgctatcag actgccccgg gtaagcgtag ccaggcaggc gagaaatgag
     2041 agggcggcca ggacgagcca tatcctgcga cgagactcga aatagagcgc cgccccgatc
     2101 cagaacacga acccggcaaa cagccccatg tggaacggcc cgctgaagaa gccgaaggct
     2161 ctgacttttc cgaaaatctg ccaggtgtag atgtcggcgg tgttggctcc gatgatggcc
     2221 aggtccagcg ccgagattgc gataaactgc ttgactgccc acagcagaat gggaacggcc
     2281 agcacggcca cgatggagag aaagtgcatc atccgacggc gtgtgccgag cagggccacc
     2341 ccgacactta ccgccgacat ctggtacaga gtctggcgga atccctcgat cccggtgcgg
     2401 aaatcgggaa tgttggggtg catgatggcc gccgcgccga ggcccagcaa ggccagcacg
     2461 gccgcctcca ccgcggtcgg gagttgcccc gcgagtaggc gcaacgacgc cgtatacagg
     2521 acgaggacca tgagcaggct gtccgcgacc aggagcacat agcggctgcc gagcgcgacg
     2581 cgggcgagat tggcatagag cgcaaagccc acaagcaaaa cgagactcgc gtagagcact
     2641 ctctgaccgc aaaccggcgc ggtgacaaac cgactgccgg cggccagtgt tctcgactca
     2701 ccagacatgg caaccaacct tctccaggcg gccgctgcag ccgtcactca ctgccctggc
     2761 gatcaagtcg tagcggtctc tccgaccggt cgcccacatg aaagcccgca gtatcgccag
     2821 gtaagcgagg cgattcagtt gccacaccca tgggcgcgtg agcaggtggc gccggacaaa
     2881 gatcacgttg ctccggacgg tgcagtagac gcggaaatcc ggagcctgcg agaggttctg
     2941 aaacacggtg acccgacgcg ctgtgctcga tacatgccag gacgggtcaa ggtcgtcgat
     3001 tcgggagccg gccacgaggt acaccgcccc gccgtcgctg atccgcgtcg taaaatcgta
     3061 gtcgtcgccg tagaggaagt agctctcgtc ggggtagccg actcggtcga ggagagtctt
     3121 gtggaacagc agcccgccgt agactccgta gggaatcttc acggatcggc tcttgaccgt
     3181 tgtgagcgca ggtcgccggc ggaacagctt gcgccaaacg cggcgcggcg catcggctgc
     3241 atgaaaatca aggaagctgt tggccttcgg gaatatcctg cggggatccg ctccggccgc
     3301 gatttgaacc aggtacgggc gatcggtgcg cagtgccagg agcgccagtc ggtcgctcgc
     3361 ttggccggcg cttagctccc gccaggcctc cgcgagcgcc tcgagggcac cggattcggg
     3421 aaagttgtcg tcatcgagaa gccacacgaa ttcggtgtcc accgcctccc tggccgcctt
     3481 catgcctata gcatacccgc gagctgatcc ctcgttgcgg ccggaaccga ctacctcgac
     3541 cctcagaccg aaagcgcccg ccgtccgccc acgcaagtcg taggttgccc cgttgtccac
     3601 gatgatgacg cgggctacgg ccggccacgc gcacagccgc tccagtaccc gcctgagcag
     3661 atgccagcgg tcgccgtacg tgacggtgac cgcgcacacg gtgagttggc cgatggccgt
     3721 ctcaagacac aatcgcgcca cgacacgcct cccgcgttga atccgccgtc atcgtttgcc
     3781 cggctgtccg cgccggccct tccgcatttc gcattaagaa gaccaatttc gccccgccga
     3841 gcacccccgc ggcggcaagg gtgccgaggg cggccccgat cgggccgtac gcaggcacca
     3901 gcagcgcgtg cagcaccagt cccgccacgc acgcggccgc gctcgccgcg acgatcgcgc
     3961 tgtcgcgccc ccgggcatag agcgccaaat ggggaatgta ggccgccccc atgagcgcca
     4021 tggccgccag cagcacccac cccgccggca attgctcccg gtagacctcc cggccgacca
     4081 tcgccagcgc cggcccaaag caggcggccg ccgcgacgct cacgagcgcg atcacgccga
     4141 gaacgccgag cgccagccgc cgcatgtggc agcggtacag cgccagatcg ccgcgctgcc
     4201 aggccccgac caggcgcggc agaacgacga tcacgaccgc ggtgtagacg aaaatctgga
     4261 ccgtgttggc gatctgggcg aaaaaggtgt acacgccgac cgccgccggg ccgtggaaat
     4321 gctggaggaa gtagcggtcg gcatactgga cgccgagcag gcagacggtc gcggcaaaca
     4381 ggcgcagcga cacgccgaca ccgcgccgga tccagttcca gtcgaccggc ttctccagcg
     4441 cctcccgcca cggcaggcgc cgcagagtcg cggccgccag ggcgatgctc gccgccgccc
     4501 cgaccgccca ggcgcaaaag acgcgcccga gcgtgaccgc ccccggcgcc gccaacccga
     4561 cgccgaccac tgcgtacacc cacacgcccg cgcgcaggaa gagcacgagg ttcgccagac
     4621 ttgaccgtga gaaagtcacc agcagccggt aggcctcctg cgagaggtgc tccagcacca
     4681 gcagcaggta gaaccacccg gcgcagcgcc acggcagagt gccggaaatg aacaccgcca
     4741 gcgtgagcgg caacaccgcc gcgtagacca gcccgtgaaa ggccatttgg tcgcgcacga
     4801 aacacggccg atcaggcggc gcagcgccga gaatctcgcg ggtgttgtag gtgtgaaagt
     4861 ccagtccgag caggtagagc gtgaccgtaa tcgtgaccac caccaggccg tagaccccca
     4921 gttcggcggg cgtgaccacc cgcgcgagaa acaacagcag gaggaatttg ctgccgagag
     4981 tgagactgcg caggagagag ttgagaaggc cggttttcag cacagcgccc ccccggtccg
     5041 gaggccggcg gcgggtgatt ggcggcgcgc ggcggcgatg tctgctcggt tgtccataac
     5101 cattttgcgg gcatttccct gcccttgtga ttaatagatt tatcatgggg cgtgccggat
     5161 cccccgcgaa aacacactcc tgcgcaccgc aacgacttac acggcggatc gcgccctcgc
     5221 agcggcgcac cgcacccggt cgcggggaaa cgatttccgg gccggcgggg agaaatcttc
     5281 cgcccgggcg gtcagtcatc aatccggaac ggcgaggcgg gatcgttctc gtggcggatc
     5341 tcatcgaccg gctcccgccg cccctccccc gcgtacagcc ggttgggagc gttgaacacc
     5401 agaccgtcga ccgcgccgac attcttgtag gcgtggacca cccccggcgg gacgatcacg
     5461 agcgcgcggc ggtgttcgcc gaactcagcg cggtaggtgc gcccgtggga gggcgcgccc
     5521 ggccggctgt cccagaggta gagacggaac gtcgacggtc cgagaaaaca gaagaggtcg
     5581 gactggtcgc ggtgctcgtg cggtccgcgg accacgcccg ggcgggtcat cgacacgtag
     5641 ctcatggccg gctcgaacgc ccccgggagc tcatcgcggc gaaagagctc ggtgagccag
     5701 ccgcgggcgt ccgccacaca gcgcagctcg cgaatgatca cgccctcgat gtcacaccgc
     5761 atagagcgca gcctccctcc ggccggcgag gaattccgca agggcctcgc gccagtcgcg
     5821 catgacatgg cagccggcgg ccttgagggc cgcgttctcc agcgaggagt aggcgggccg
     5881 cggagccggc cggcccagcg cggcggtcgt gcaggggcgc aggtcaaccg ccatcccaag
     5941 ccgcgaaaaa atgaatgccg ccaggccgta ccatgaggtc tccccttccg ccgagcagtg
     6001 gtacaacccg gtgagccgct cgcgcaggac gatttccgtc tgccgcacga catccctggt
     6061 ccaggtcggg ttgccgacct gatcgtcgac aacttgcagg gaggcgatac tctcgccgcg
     6121 ccgggccgcc tccagctgct cggcgccgcg cgcgagcatg gtgacgacaa agttccggcc
     6181 gtgcgcgccg tacatccagg cgaggcggag gatggcgctg tcgggctgcg cggccgaggt
     6241 gcgccgctct ccctcgagct tgctccgtcc gtagaccgtc cggggattgg ggggggcgct
     6301 ttcgacatag ggtgtgcgcc gggcgccgtc gaaaacataa tcggtggaat agaaaaccag
     6361 gtgtgccccg accgcgcggc aggcctcggc gacgaatgcg gcgccgtcgg cgttgacccg
     6421 tatggcccgc tccggctccg cctcgcaccg gtcgacctcg gtgaacgcgg ccgcgtgaat
     6481 aacgacggtg gggcggacgg ccgcgacgtg ctgccggacg gcggccgggt cggtgatgtc
     6541 gaaatcgtcg aggtcgcagc cgaccacgtc ggcgcgccgc cggaactcgg cgacgaggtc
     6601 ggtccccagt tgcccgcggg cgccggtgac caggagccta tccctcaccg ttgacctccc
     6661 ggacaacgtc gcgcaggtac tgggcatagg cggtgtcggg gatggaggcg aggagcgcct
     6721 ccatctggcg gcggttgatg tagcgcatgc ggtaggcgat ttcctcgatg caggcgatct
     6781 tctgcccctg gcgcttctcg atcgtggcga tgaaattgct cgcgtccagc aggctctcgt
     6841 gggtgcccgt gtcgagccac gcgatgcccc gcccgagccg gacgactttg agccgccccc
     6901 gccgcaggta gtgattgttc acatcggtga tctcgagctc accgcgggcc gatggccgca
     6961 gcgaccgcgc gaccgagacg acctccgcat cgtagaggta cagcccggtg acagcgtagt
     7021 tggacttggg cgccgccggc ttctcctcga tcgagatcgc gttgccgtcg tggtcgaact
     7081 ccacgacgcc gtaccgctga ggatccttga catagtagcc gaacaccacc gcaccgtcga
     7141 cgaacgcccg gacgccgcgg aggaaatcgt agaccccgta gaagacgttg tcgccgagaa
     7201 tgagggcgac gttgtcgcgg ccgatgaaat cggcgccgat gaggaaggcc tgggcgattc
     7261 cctccgggcg ctcctggacc gcgtagcgaa gcgtgagccc gaccgcccgg ccgtcgccaa
     7321 gcagatcccg aaaccgcggc aggtcctggg gcgtgctgat gatgaggatc tcggtcacgc
     7381 cgaacagcat cagggtcgac agcggatagt agatcatcgg cttgtcgtag atcggcagga
     7441 gctgcttgca cgcaacctgc gtcaccgggt ggagccgcgt cccggccccg ccggccagaa
     7501 tgattccctt gcggatcccg gcgctcaccg tcgcaccccg ctagcggccc gcatagatgt
     7561 cctcgtagta ttggaggtac tgcccgctga tgcagtggtc gagccactcc tggtggtcca
     7621 ggtaccacgc gatcgttcgg cacaggccct cggcgaatgt gaccgagggc cgccagccga
     7681 gctcgcgggc gattttcgag gcgtcgatgg cgtaccgccg gtcgtgcccg aggcggtcgg
     7741 tcacgaaggt gatgaggttt tcgcgggggc cgccccccag ccggtcgtca agcattcggc
     7801 agatcaggcg gaccaactcg aggttctcga tttcgttgtg cccgccgatg gtgtaagtcg
     7861 cgccggattt cccctcgtgg aacaccaggt cgagcgcccg gcagtggtcc tcgacgtaga
     7921 gccagtcccg cacattcttc ccgtcgccgt acaccggcag cggcagcccc gcgcgggcgt
     7981 tgcggatcat aagcggaatc agtttctccg ggaactggta ggggccgtaa ttattcgagc
     8041 agtgggtcgt caccgtgtcg aggccgtagg tggccgcgta ggagcgcacc agatgatcgg
     8101 ccgccgcttt cgaggccgag tagggggagt tggggcggta cggcgtgtcc tcggtgaaat
     8161 gcccctccgg cccgagcgac ccgaacacct cgtcggtcga cacctggtga aagcggatcg
     8221 cctgcggtcc ggaccgctgc gttttcttgc gcgccagttc caggaggttg aaggtgccga
     8281 ggatgttggt ccggatgaag gtcgacggtc ccaggatgga gcggtcaacg tgcgattcgg
     8341 cggccaggtg gatgatgccg gacggatcga aagcatggaa acagtcggcg aggcggtcga
     8401 agtcggtgat atcgatccgc gcgaaccggt agttgggggc tttctcgatc gctttcaggt
     8461 tggccaggtt gccggcgtag gtcagacagt cgacattgac ccaccggcag tccgggtagg
     8521 cgggcaccag gtgcaagagg aggttggaac cgacaaagcc ggcgccgccg gtcaccacca
     8581 ccgttcgctg aaacgtttcg tcacccatgg gtatggccga tctcgaggtt tacgcgcagg
     8641 aagtcttgcg gatatcggcg gtcggaacaa caaaaaaaag aggccggaac ttatcggcaa
     8701 gaaatggcgc cccgcggaat cgaaccgcgg acacacggat tttcagtccg ttgctctacc
     8761 atctgagcta gggcgccgcc gatcggaacc gatcagtagc cgaagatcat gcgctttccc
     8821 cggcctgtca acccaaaagt taccgccccg gaaacaaata ccgcttcgtc gcactcgtga
     8881 gaagcagcgc gagcgcaacc gcggcgaggg ccaggatgat caggttggcg tagttgcgaa
     8941 aacccgccag ggccgcgatg atgatgaggc cgttgatgcc gatctcgccg taccaggccc
     9001 agctccgccg attgcgcaaa ccccacgccg ccgccatcag caccccgccg atcagcagaa
     9061 acccgaacgg ctgggcgaga ttggcgtact gccggtcgag aaagctgagc accagcgtga
     9121 ccaggccgta gagcaggaac acggtcgcaa acccgaacgc ccaccagtag gcgagtttga
     9181 acggcaccgg ccgcgtgtcg ctcttctccg tcatgtcgtc tccgtcctat gcggcgaact
     9241 cgccgagcag gccggcgccg ataaacccgg cgtcgttgcc cagcgccgcc ttgaccactt
     9301 tcaaattctg tacggcggaa tcgaaagcca gttcccggat cttggcggcg acaatttcga
     9361 caaacccgcc gcccccttcg gcaattccgc cgccgatcac gatcatttcc gggttcagga
     9421 gattgacgac gccggccaag ccgatcccga gataggtggc cgcctcggta atcgccttcc
     9481 gggccgccgc atcgtccttc cggagcgccg cgaagatctt gcggatgctc aggttgtcga
     9541 ggtttccctc gagcacagcc tcaaagcccg gcgtcatctc gcgctccagc tcgcgccgga
     9601 gccgcccgag gatcgcctgt gaggagcagt acccctcgat gctccccggc atgccgccgt
     9661 gcgtgaacgg ggcgtcgaag ttgatcgtca tgtgcccgac ttcgccggcc gaccacgacg
     9721 agccgcgcca gatgcggccg ccgaaaatga ccgcgccgcc gaccccggtc ccgactgtca
     9781 ggcagagcgc cgactccgcg ccgtgggcgg cgccgaagtg cgcctcggcc agcgccatcg
     9841 cgttgacatc gttgtccacc cacaccggca cgttgaggcg ttcgcggaga ccggccccga
     9901 tctcggtccc ctgccagccg gggatgttcg gacaggggcc gataacgcgg ccggtgtgaa
     9961 aatcgaccgc ccccggcgtt cccaccccga ggtggcgcac ttcgtaatcc tcctcggccg
    10021 ccgtgtacag caagcgctcg ccgatgttgg ccgccaggtg catgagcggt ttcggtccct
    10081 tctccaccat cgtcggcttc tgctcccgga actgcacctt cccctgctcg tcgaacagcc
    10141 catacttgat ggtggtcccc ccgatatcaa tgccggcgaa aatctgtcca gctgccatcg
    10201 atccgtactt tgtcgcggcg ggcgacaggt ggcccgtcgg ccgtgtcgtc ctcgaatagc
    10261 gcaccttgtc tcgggcggca cgggtgcccg gactccgccc cggggtcaaa aacgctgata
    10321 tatagacgga ggctaaagag gtgtcaaggt aaaacgagcg ggtcttgcgg gctgggggcc
    10381 gccgcctcta actctggtgt tcctgttgct cccgccagtg gcgccactgc gccgcatagc
    10441 ggctgatcca gtcctccact gcgccctggc cgagatcgtg ccccgcccgt tccgactcaa
    10501 tccacttgtg cttctggatc tccgagcgct gggcgtccaa ctcctcctgc agccgggcct
    10561 gccgccacgt cgtcgagtgg tggatcagcc aatgacggac cgcatactcg agaccaacgt
    10621 cggtcccggc ttcgagcgag agaaagtatt tgtgcacctc aatcgcccgg gcttccgccg
    10681 gcgagaattc ttcaaggagg tgatgcggat gcgcgcagcc gttgtccatg tccagtatct
    10741 tcggaaaatc cccgcttcca tacggtttgg cgtaataaag gccaaaatgc ccattctgtc
    10801 aaggcacctt cactgcctgt atcggcagag gcagggcaag tatggagcgc cttcccgacc
    10861 gaccggctcc ggcgcaagcg agcgcttgtc gatcctcggc ggccgacgta ttattagggc
    10921 catggccgac gctcacgacg aacatcgggc ctcgcgctgg tccctcccgg ccaggtacgg
    10981 ggccaaccgc gaggtggtcg ccctctcggt cgcccgcctg gcggaggcgc tcggcaacag
    11041 cattctcacg atcgtcattc cgctctacgt ggccgagatc gcccaccccc tgttcaacct
    11101 tcccgtgccg gtcctcgtgg ggaccctgat ttctctgtac ggcatcgtct ccgcggcggt
    11161 gcaaccggtg atgggggcgc tctcggaccg gttgggccgg cggaaagtgc agattcaggc
    11221 cgggatggcg ctcatggggc tgggtacgct ggccttcatg gccgccacct cctactcgca
    11281 cctgctcgtg ctgcgtgcga tccaggggat cggcgtggcc accaccattc ccgcctcgat
    11341 ggcggtcatg gcgaccgcca cccgccgcca cacccgggcc tcctcgatgg ggatctactc
    11401 gactctccgc ctgatcggat tcgccggcgg acccgtcatc ggagggtttc tcaaagtgcg
    11461 cttcggtttc gatgccgcct tcatcgtcgg tgccgcgttc gtgctgctgg ccatgatcct
    11521 cgtccaggtg tgggtgcgcg agacgccgcc ggctgaaccg gccgccccgc ccgcacccgg
    11581 ccggctgcgc atcctcgacc gcgacctgct cgacccggga attctcgccg ccgccctcgc
    11641 caccctgctc atggccgcca atttctccat ggtgaccacg ctcgaaaacg aattcaacgc
    11701 ccggctcgac atgaccgcgt tcgagttcgg catcgccttc gccatggtca tggtcagccg
    11761 cctggtgttt caggtgccgg tcgggcggct ggccgacacc atcggacgca aaccgctcat
    11821 gctgggcggc cttctggtgc tggcgccggc gaccgggctg ctgggctacg taaccagccc
    11881 cgcacagttg atggcggtgc gctttctcca ggggtgcggc ggggccggca tcgccgcacc
    11941 ggcgttcgcc gtggccggcg atctcgcccg cgagggaggc gagggccggc aaatgagcct
    12001 cgtcaccacc ggtttcggcc tcggcatgac catcggcccc ctcatcgccg gacccctttc
    12061 cctgttcttc ttccaactcc ccttcctggt cggcggcgcg ctcgccctgg ccggcctctg
    12121 ggcagtgtac cggtacatgc cggagacggt cgccggcgga cgcggctggt ttcgctccgc
    12181 cgccccggag aaagtttcag atcgaaacgc cggctgaccg ccgacctgtt tgtccggccg
    12241 ccggctcccc ccgatccgcc cccttgaggc ccgttccctt tcggccgata agaggaaaaa
    12301 gacgctggaa ctcggaatta tcaggggtga gagacatgaa tgaccggcgc gacaatgcgc
    12361 ggcgccgcac ggcggcctac catctggtgt acgaacttga gacgggcgag tttgtcgggc
    12421 gcgtgatcaa cctcaccacc ggcggcctcc gcctgatcag cgacgccgcg gtcgccgttc
    12481 ccacgaaatt gagctgccgc atgcgcctgc cggatctctt caagggcgcg cgcgagatca
    12541 tgttcgatat cgagagccgc tggtgcgagt acaaccgaat cggcgattgg tacgaaacgg
    12601 gctatgagtt tgtgcggctg ggcgagctcg cccgccaggc aatcgacgcc atgatccgcg
    12661 attggccccg cgagacgacc cctgccaaga cccgctgagc ctgccccgcc ccgaccgcgg
    12721 ccgtcaccgc accggaatca gcagatccgt gatcagctcc gcgggagggg tgaccgcatg
    12781 atcctgcgga gcgtatatct ccaggcaggc cccgccccgg ctgtgcctcc cgctcgaggg
    12841 caaccactcg tagagcaggc ggcgaaaagc ctccgccaga ccgctgtagg gaccgacgtg
    12901 ccgatagacg gcgtactcgc ccccggggaa cgtctgcacc ggaatctcgt ccaccgggtg
    12961 aaaatcctcc tcaacctcga tgcaggcatc gtagcgcaac agcgccgccg gcacgaccgc
    13021 gggattgtcc cacgaggcgc cgatcaggcg gaggcccgag cgagacagtc cccgttcgcc
    13081 cgcccattgg cagagacggg tgaacgtcgg cccgacctca tggtacggac cgatgtggcg
    13141 catataggcg acccgaatcg gattcaggcg ttgcacgata acatccggac ttcccgacat
    13201 aatgcacctc ctgcggttgt tcctggagcg ccggccatgc cgccatcata tcgccccggc
    13261 cgcccgacgg caatcacatt agttcccccc ggcggtcggg ccgcgccggc agcgggcccg
    13321 acccggggag ttcgaacgat caggatttcg ccgccgtctc ccgcgccatg gcctcgaccg
    13381 cggcggccat ctcctgggga gaaacgtccc gcacgtgcga cgcgaacgac cagtggtggc
    13441 cgaacggatc ctccaccgcg ccgtagcggt cgccccagaa catgtcttcc ggcggacgga
    13501 cgaccttcgc gcccgccttc cccgcccgct ggatgatggc gtcgcagtcc tcgacgtagt
    13561 ggtggatcgt caccggcgtc ccgcccaacg ccttgggcgt ctgcgatcgg ccgccgcaca
    13621 tttccgggaa atcgtcgcag aggtagatga cgctcgcgcc cagcctgatc tcggcgtgga
    13681 acagccgccg gccatcgggc gccggcatcc gcataagctc ctcggccccg aacgccttgc
    13741 ggtagaagtc aatcgccgcc gcggcgttgt caacgacgag gtgcgggatc gcccgctcct
    13801 gtcccgtggg aacacgcttc gcctgggcca tggggccctc cttgtagtgg ttgttatgga
    13861 ccaattatat cgactataca tcattatacg gagaccgatc ccgcaccgcc atcagctttt
    13921 ttggggcggg cgcaagattt ccgccgcatg cgggcgaggg cgccgcggcg ggggcgggcc
    13981 tgcccgttgc cggccaaaag aaaacccccg accatgcctg gtcgggggct tgcttactcc
    14041 cggcagcgtc ctactctccc acgcagtctc ccgcgtagta ccatcggcga tgatgggctt
    14101 aactgctgtg ttcggaatgg gaacaggtgt ttccccatct ctatcgccac cggaaaattc
    14161 tcatagatct ttcgacgcgt accgaaaccg ggcccgccgc cgtcgccggc gccggggttt
    14221 cgatctactc gggttgatcg gaggttcgca tcgcccaaac ggctgtgtca tcagcgcgtt
    14281 cggatgcgcg aacgtaaggg tttctgtagt cattacttcg gcgcggggcc ggaagtaatt
    14341 ttacggttaa gtcgcacgac cgattagtat cactaggctc aacgcatcgc tgcgcgtaca
    14401 ccagtgacct atcaacctgg tcatcttcca ggggtcttca ggaaccgaag ttcgggagat
    14461 ctcatcttgg agtgggcttg gcgcttagat gctttcagcg cttatccctt cccggcgcag
    14521 ctaccctgcg atgcaactgg cgtcacaaca ggtgcactgg cggccggttc atcctggtcc
    14581 tctcgtacta gggacgactc tcctcaaatc tcctacgccc gtatcagata gggaccgaac
    14641 tgtctcacga cgttctaaac ccagctcacg taccgcttta attggcgaac agccaaaccc
    14701 ttgggacctt ctccagcccc aggatgcgat gagccgacat cgaggtgcca aaccccctcg
    14761 tcgatatgaa ctctcggagg agataagcct gttatccccg gagtaccttt tatccgttga
    14821 gcgacggcgc ttccattcgc aaccgccgga tcactaagac ctgctttcgc acctgctcga
    14881 cgagtcagtc tcgcagttaa gctcccttgt acctttgcgc tctacagccg gttaccattc
    14941 ggcctgaggg aacctttgca cacctccgtt acactttagg aggtaaccgc cccagttaaa
    15001 ctacccacct gacactgttc ctggacccgc tagagggccc gaggttagat gtccaatgac
    15061 acaagggtgg tatttcaccg gtggctccac cgacactagc gtgccagctt caacgcctcc
    15121 cacctatgct acacatgcga caccaaacac caatgtcagg atatagtaaa ggttcacggg
    15181 gtctttccgt cccgatacgg gaaaacggca tcttcaccgt tgctacagtt tcaccgggcc
    15241 tcacgttgag acagtgccgc agttgttata ccattcgtgc aggtcggaat ttacccgaca
    15301 aggaatttcg ctaccttagg acc
//
LOCUS       DAOPOE010000077        15317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4604_length_15317_cov_5.290919, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000077 DAOPOE010000000
VERSION     DAOPOE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_15317_cov_5.290919"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /locus_tag="PKM94_10265"
     CDS             <1..1881
                     /locus_tag="PKM94_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD67058.1"
                     /translation="YGFPWIQNPSSFTSVGCQLPTEVKFPLLIHLQDNVTVSNGATWA
                     NDTLYFTPPSEGTHTVRVIAENSYGADTADVAINVTIDIPMNIDQTDMEFTTTDTSTV
                     MPPAQWIHVNSSCGDGMLNWTVEPHFDVEWLEIDKLSGTNPDSVRVALKGDPGSWAPG
                     IYRATLVFRDIAGRHIQTIPLALFVESGVTVGTQYTQPGRTVTVPVYLHTNDTLRDFT
                     IPLRYFTTQSAAVVVDSVSFSHAIVDTAWIIPDQQIIIVDRDIQPPPMPDSTDSMMVP
                     QTCKVADIHFTVRDFAYDEKVLIDTTTYVAPDDDTLRYRFVLATGDTTVPAFSPGQII
                     IGEEPPCCVMRGDFTHDWPPELTILDLTSLIAYLFRGGAAPQCLDEADVNADGGVTVG
                     DATMLICIIFRDCQTPLACGVVKSARRAAPEPIASLRTEFDGAATTVILEASEPLMGV
                     QITLQADHPDSIANTTETGLELVHGTVDGLTRVGLLDLQGEVHLPQGSTTLFTVPGEV
                     TLVDGLVADMSHQSMIARIGGKSSSPNLPRRFELAQNYPNPFNPTTVIQFALPVATHV
                     RLEVINILGPTLVDRQMEAGYQSVEWNSTNSDGSRVASGVYFYRLEAGTFRDTKKMLL
                     LK"
     gene            complement(2057..2144)
                     /locus_tag="PKM94_10270"
     tRNA            complement(2057..2144)
                     /locus_tag="PKM94_10270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2108..2110),aa:Ser,seq:cga)
     gene            2542..4665
                     /locus_tag="PKM94_10275"
     CDS             2542..4665
                     /locus_tag="PKM94_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOD67059.1"
                     /translation="MKDPHFSPFVPPDRSLREFTFRAVALGAILGIVFAASSVYLALK
                     VGMTVSASIPIAVLSITLFRVLGRATILENNIVQTTGSAGESIAFGVATTMPVFLLLG
                     QDMELLSICWMALLGGILGVLMMIPLRQGLIVKEHGRLTYPEGTACADVLIVGEQGGT
                     NAKTVFLGFGLGAAYKFLNAGMKLWAEIPNRMLGFFKGASVAAEITPELLGVGYIIGR
                     RVSANMMAGGLLSYLILIPAITIFGENLTAPMFPATTLIRDMSPNEVRNAYVLYIGAG
                     AVATGGIISLIRSFPTIIAAFRRGFRTFLDSRAGKGAQEAVLRTERDLPLSVVVFGSI
                     GLVLAIWLSPILHINVISAVLIVLFGFFFVTVSSRITGEIGSSSNPISGMTVATLLIT
                     CLLFLAVGWTGVSYRAMALSTAAIVCIAASNGGTISQDLKTGFLVGATPRLQQIAIMI
                     GVISSALVIGLIVIALNNAYTTVTPARHPDYLAAAAPGAETQQGPDGAMYRVHYVNEP
                     VGTVLIGKYLVDDSGRIAYLVDPGIAGVVSEVDGKQVTKLDSPKARLFSLIIDGILTQ
                     KLPWGLVLIGVFLALMMELVGISSLPFAVGLYLPLSSSAPIMAGGLIRGIVDRKRKSN
                     SAEAEFSPGVLMSSGFIAGAAIMGIVVAGLTGFGLDKAINGSGWSHYLSEADWFSLIP
                     FGALMYMLYRVGVSVKDKGKQKSAA"
     gene            complement(4810..5346)
                     /locus_tag="PKM94_10280"
     CDS             complement(4810..5346)
                     /locus_tag="PKM94_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67060.1"
                     /translation="MRDEGNEDRYDLARMIAGSLYQGLFINILLPAGLLLAAYVIEQR
                     GGLGNALGAAANPLFYVLAVVSVAEGAAAVLWRSKTLALPMVRRIETLEEDIRGELRV
                     RLRPVFLLIAGIALNGFAYFLLTGRFREGLVFVVLSFLAFQIVRPRYGMVRGLIKQQE
                     DLAARGLLRTQRRPGELT"
     gene            complement(5343..5813)
                     /locus_tag="PKM94_10285"
     CDS             complement(5343..5813)
                     /locus_tag="PKM94_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67061.1"
                     /translation="MKPLSETQERTWMVIRVVAAAVGIVAPAFFFAIAALVHPDSSPL
                     GENGAGAIDVAFYLLLILGIVDPLLALLIERPLLADWRRRPGRRTADRMFFHLAVLRM
                     AFVEATFLYGFVVYLLTWDLMRMLIFYLVGLVWLGLYWPRREKYEQFIEGAERL"
     gene            complement(5810..6262)
                     /gene="tadA"
                     /locus_tag="PKM94_10290"
     CDS             complement(5810..6262)
                     /gene="tadA"
                     /locus_tag="PKM94_10290"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOD67062.1"
                     /translation="MRLALREAEQAFEEGEVPVGAVVVQGDRVIGRGANRTVHLHDAT
                     AHAEMIALTAAANALGDWRLEGCELYCTLEPCAMCAGAAVLSRIERIVFGSRDPKFGA
                     CGSIFLIPGDPRLNHRAEIVEGVLADAAAELMRAFFQRLRQAKGEEVT"
     gene            complement(6372..7511)
                     /gene="hemW"
                     /locus_tag="PKM94_10295"
     CDS             complement(6372..7511)
                     /gene="hemW"
                     /locus_tag="PKM94_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOD67063.1"
                     /translation="MPFGLYVHFPFCRNTCSYCDFYKEKHQAALEREFYHALRIETEL
                     AAEHPAVQRSQVTTIFIGGGTPNISDPDLLTDWLGHLRRHIDVADEIEFTIECNPEAV
                     TREQLERLKGLGVNRPVFGVQSFNTRLLKLLDRRHHPHDSYRAIYYANALGFRAWGVD
                     FIFGLPGQTSKMLAADLDQLLDFEPPHVSYYQLTVEEGTPLWRRVKAGKLRMPDQELL
                     LALYRGGVEQMADLGYLRYEVSSFARPGFECRHNLGYWEGAEYLGLGPSAHSYLDNTR
                     FANWPNVREYIKALQAGILPRTVDESGAPERMTEAIMLGLRTARGISRSAFAERFGQP
                     IESRLDTEQYALLVESGHLIPERGMLRLSEEGIHLADEITRRLLK"
     gene            complement(7515..8240)
                     /gene="lepB"
                     /locus_tag="PKM94_10300"
     CDS             complement(7515..8240)
                     /gene="lepB"
                     /locus_tag="PKM94_10300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD67064.1"
                     /translation="MEEDFLIQDIKRAEAHKEAERVRRYRAPRPVWREYLDTAILAFL
                     AAVLLRMFVVSAYRVSSSSMEGTLYEGDYIFVNKLAYKYGGAPKVGDIIVFAYPNNPT
                     KDYIKRIVALPGQEVVVGDKVLYVDGAVAEIPAHAQHTDQRILPADLSFRDNFGPYVV
                     PENQYFVMGDNRDDSRDSRFWGGVPAQNVKGKAVFVYWSWQPDDGAPGFSFPYVIDAV
                     QWIGYVVFNFPSHVRWDRVGLAL"
     gene            8573..10993
                     /locus_tag="PKM94_10305"
     CDS             8573..10993
                     /locus_tag="PKM94_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67065.1"
                     /translation="MHRLRIASWLVLGLSLLGPAEQVRAQNCDSVPFSRDEVGLQTIV
                     GLPGDTIWAPVWAITDSTLIAFRMVIQFDSTILRPLFAAPGSDQLVVEPAGRFAGSVN
                     LAASLSEAEIDSGAIAVVFLPFLNSNDTIPAGRGVILRIPFVVQQEALFAEYSLIRFP
                     RRTVYLVDSSSAPPDSTAICRGTDFVERSGGPSGMAEGFPRPSTAYFFTDPDPAGPPR
                     IEYFGIDPRSAWQGDTVQAVWSVRNADSIVLAPAVGMHPGSVQTEPFEVVEGAPTTYT
                     LTAFGRGAPVSRTLRLLAVPPGANRFPYWQYYPEPVGGPVLSPIRRYLWAIDPDGDEP
                     TFSSPNLPPGAVLIALGGGGVLFEWTPSAADTGYRAVSILTHDALDSARCDSLNLEIY
                     VRDANFPPTYSYDSSDRMIFEGDTLRIPIAAADPNGTIPSIAAHLHYADTLAANMSFV
                     DSGNGAGLLTFLPSYFQGNLAPTYYLVSFTIRDAVDPALTVATAPVRIYVQNRNSGLG
                     PPVLSLSAGEGPFTVAEEDSLVFELTAATSTGDIPVISASALPPTASLTDYPGATAGD
                     HKIFRFKPADGMVGEYRVTFYAANVDLIDSAAVDLAVVARNHPPVIMLLPGNDVIFEE
                     DTARVRVLAFDPDSVPSVLTAYLDGTDTLARNMTMVDSGNGAGVLTFVPNRVQGSPCG
                     PVFYYLRFRATDPIPPHPSVISSVRTIRVYDSGLPCCIGRRGDVNYDSNGLGAPTVAD
                     LTGLIAFVFRPGAPLPCYQEGDFDSSGAIGVVDITTLIGYLFQGGPPPTECPAARSPG
                     DSGPGCRE"
     gene            11073..13751
                     /locus_tag="PKM94_10310"
     CDS             11073..13751
                     /locus_tag="PKM94_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOD67066.1"
                     /translation="MYPCCSPRRAAIVAALLVAVLPAAPLGPPAAAAGVGSAERGSKA
                     FSAQPIFTFDTQWRVEHIETADLNGDGIPDIIAGEQNTDYYGDPHNVFAVDGATGDTL
                     WRYPLQDAVRSMTIGDVNNDGVMDVIAGASYNASNTPDGRVHAINGENGAPLWTYPVN
                     NTVSCVAVGDFDGDAFADVVAGSFDDNIYAIAGVDGSYLWSVTIGSLWINDVAAADVN
                     GDGIDDVAYAHEYLAGYTNHIGVRDGIDGSVIWDLPVAYVPTRVLYGDFDADGTLDLA
                     IGAMDELDQGWVFLRSAASGGLIWDYQIFSVEHVNGLVTLFSWDLDGNGDQDLVVGNY
                     LGAYHVYAFDGPTASLMFTSEPLTSFPQELAFGDVTGDGALDIVCSAWDRVEVLGADD
                     GKKLWYYSVAGLIKDVAVGDFDSDGVLDVAAGGGAEYVGTNPGKGVWALRTVYSPLLW
                     EYVFNQYGNELTICNLNDDAYLDVVAVMSLGDAALAINGLTGQELWTWVGTENLYAVT
                     SGDFNSDGVDEVAVAGADKRVTALGVVDKAVQWQFTTPTAQIYRKCLASSDLNGDGAD
                     DVIAGCDDGHVYAINGLTGIALWDAPSLGAVNEVELAQMNDVGPLDVVAASSTGKVWV
                     LNGETGAVLWDYTTGGTCEHVEVLDVNGDKVNDIAAGVAPSAARVIMINGATHLEMWN
                     VSLGLASNTHSFAHGDLSGDGLDDLVAPGNSTDRKVHALWGLTGGDLWTPFLTGDEVN
                     CVWVQDMDGDGVNDVIAGSDDQLVYVLHGNTGVPYWQFSTSGDVMHLMVGDIAGPGRP
                     SIACVTFDGDGTVYAFDSWYVEPGCCTLRGDYDSDGAIKVADLTQLVAYLFRGGPAPG
                     CFEHGDIDGDGTIKVSDLTLLISYLFRSGPPPAACP"
     gene            14588..14821
                     /locus_tag="PKM94_10315"
     CDS             14588..14821
                     /locus_tag="PKM94_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67067.1"
                     /translation="MRVKFVLTMDNLVVDDKSIDQVVLDWEANVDQSEILQLSHQWIT
                     SRNFLTQRMLGLVQVGESSLTIEPLEEDDYAQW"
     gene            14808..15143
                     /locus_tag="PKM94_10320"
     CDS             14808..15143
                     /locus_tag="PKM94_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67068.1"
                     /translation="MHSGEWLAQARALLERAVQAVQDLAAAAAGAQEWIVAHFGQNGL
                     YAAAFIGAALGLYLILQLIRITLAALKYLVVPGVGLALLGSLVLPFSFFFLLPITLSA
                     CSLVLLFKG"
BASE COUNT         2577 a         5326 c         4731 g         2683 t
ORIGIN      
        1 tatgggttcc catggattca gaacccgtca tcgtttacat cggtcggctg tcaacttcca
       61 accgaggtga agtttccact gctcatccat ctacaggata acgtcaccgt ctccaatggc
      121 gccacctggg ccaacgacac cctctacttc acgccgccgt cggaggggac gcacacggtc
      181 agggtgatcg cggagaacag ctatggcgcg gacaccgccg atgtcgcgat caacgtgacg
      241 atcgatatcc ccatgaacat cgaccagacc gacatggagt tcacgaccac tgatacctcg
      301 accgtcatgc cgccggcgca gtggattcac gtgaactcgt cctgcggcga cggtatgctt
      361 aactggacgg tggaaccgca cttcgatgtg gagtggctcg agatcgacaa gctgagcggc
      421 acaaaccctg attcggtccg ggttgcgctc aagggggatc cggggtcgtg ggcccccggt
      481 atctaccgcg ccacgctggt gtttcgggac atcgccggcc gtcacatcca aacgatcccg
      541 ctggcgctgt ttgtcgagtc cggagtcaca gtcggaacgc agtacacgca gccggggcgc
      601 accgtaactg tgccggttta cctgcacacc aatgacacgc tgagggattt caccatcccc
      661 ctgcgctact tcacgaccca gtctgcggcc gtggtggtcg actcggtcag tttcagccac
      721 gctattgtcg acaccgcctg gatcatcccg gaccagcaga tcatcatcgt tgatcgggat
      781 atccagccgc cgccgatgcc ggattcgacg gattcgatga tggtgccgca gacctgcaag
      841 gtggcggata tccacttcac ggtgcgagac ttcgcctacg acgaaaaggt cctgatcgat
      901 acgaccactt acgtggcgcc ggatgatgat accctgcgct accgcttcgt actggcgacc
      961 ggcgatacga cggttccggc gttcagcccg ggccagatca tcattggcga ggagccgccg
     1021 tgctgtgtga tgcggggcga tttcactcac gattggccac ccgagctcac tatcctcgat
     1081 cttacctctc tcatagccta cctcttcagg ggcggtgcgg cgccgcagtg tctcgacgaa
     1141 gccgatgtga acgctgatgg tggggttaca gtgggcgatg caaccatgct catatgcata
     1201 atattccgcg actgccagac tcccttggcc tgcggggttg tcaagtcggc ccgccgggcg
     1261 gccccggaac cgatcgcctc gctgcgcacc gagttcgatg gcgccgcgac gacggtcatt
     1321 ctcgaagcct ccgagccgct catgggcgtg cagatcacac tgcaggcgga tcacccggac
     1381 agcatcgcca acaccaccga aaccggcctt gagctggttc acggcacggt cgatgggctc
     1441 actcgtgtcg gcctgctcga tctccaaggc gaggttcacc tgccgcaggg atcgacgacg
     1501 ctgttcacgg ttccgggcga ggtgacgctg gtcgatggcc tcgtggccga tatgtcgcac
     1561 cagtcgatga tcgcccggat cggcggcaag tccagttcgc cgaatctgcc gcggcggttt
     1621 gagctggcgc agaactaccc gaacccgttc aaccccacga ccgtgatcca gtttgcgctc
     1681 ccggtggcga cccacgtccg gctcgaggtg atcaacattc tcggcccgac gctggttgac
     1741 cgccagatgg aggcggggta tcaatcggtc gagtggaaca gcaccaactc cgacgggagc
     1801 cgggtcgcga gcggcgtgta tttctaccgc ctggaagcgg ggacattccg cgacacgaag
     1861 aagatgctct tgttgaagta gctgctggac acggaaacgg caaacgaaag gggcggccgc
     1921 cggccgccct tttctcattt gccggccggt ggcgacaata cggtggaagt ttgccgcctt
     1981 ctcccggtca aattgcctga atgcgcactg gacctcccgc tttcccccgc cgcaacctcc
     2041 gcgccctcac acaaaatggc ggagaggctg ggattcgaac ccagggtacc cgagggtaca
     2101 acggttttcg agaccgccgc tttcgaccac tcagccacct ctccgtgtct tcgcaggcgc
     2161 cggggccgaa agccccggtc gccgcgacta tccaatttaa tcttccgcca ccgtttgtaa
     2221 agtccaattt ccgcccgcct cggcccagag cagccgcatc ccggctcagg ccacccggcg
     2281 cttccaacca gcgccggcga agcctgttca gcactaacca gccgggcgtg tcgcgcgagg
     2341 gtcggccgaa ggcgttgcgc gagggtcggc cgaagacgtt gcgcgggggc tcgccgagaa
     2401 cattacgcgg agccggcccg gggtgtccgg ccgtggagga gcgggcgccg ccgcggggcg
     2461 ccgcaattaa ccgattgccc cgccgcagac cattgcgtat acataccgga accaccagcg
     2521 tgcccttgca ggagagcaaa gatgaaagac ccgcacttca gtcccttcgt cccgcccgac
     2581 cgcagtctcc gcgagttcac ctttcgcgcg gtcgctctcg gggcgatcct cggcatcgtc
     2641 ttcgccgcct cgtcggtcta cctggctctg aaggtcggca tgaccgtgag cgcctcaatc
     2701 cccatcgccg tgctctcgat cacgctcttc cgcgtgctcg gccgcgcgac cattctggag
     2761 aacaacatcg tgcagacgac cgggtcggcc ggggaatcga tcgctttcgg tgtggccacc
     2821 accatgccgg tcttcctgct gctcggccag gacatggagc tgctgagcat ctgctggatg
     2881 gcactcctgg gcgggatcct cggcgtcctc atgatgatcc cgctgcgcca ggggctgatc
     2941 gtcaaggaac acggccggct cacctatccg gaggggactg cctgcgccga cgtgctcatc
     3001 gtgggcgagc agggcggcac caatgccaag accgtctttc tcggtttcgg gctgggcgcc
     3061 gcgtacaagt ttttgaacgc gggcatgaag ctgtgggcgg agattcccaa ccgcatgctg
     3121 gggttcttta agggagcctc ggtggcggcg gagatcacgc ccgagctcct cggcgtcggc
     3181 tacatcatcg gccgccgggt ctccgcgaac atgatggcgg gcggcctgct gtcgtacctc
     3241 attctcatcc ccgcgatcac gattttcggc gagaacctca cggcgccgat gttcccggcc
     3301 acgaccctca tccgcgacat gtcgccgaac gaggtgcgca acgcctacgt gctctacatc
     3361 ggggccggtg cagtcgccac cggcggcatc atcagcctca tccggtcctt ccccaccatc
     3421 atcgccgcgt tccgccgcgg cttccgcacg ttcctggact cccgcgcggg gaagggggcg
     3481 caggaggcgg tgctccgcac cgagcgcgac ctcccgctct cggtcgtggt ctttggatca
     3541 atcggcctgg tcctcgccat ctggctctcc cccatcctcc acatcaacgt catctcggcc
     3601 gtcctgatcg tcctcttcgg gttcttcttc gtcaccgtct ccagccgcat caccggcgaa
     3661 atcggctcct cctcgaaccc gatctcgggt atgaccgtgg ccaccctcct gatcacctgc
     3721 ctgctcttcc tggccgtcgg atggacgggc gtgtcgtacc gggcgatggc tctctcgacg
     3781 gcggcgatcg tctgcatcgc cgcctcgaac ggcggcacaa tcagccagga tctcaaaacc
     3841 ggcttcctcg tcggggccac gccccggctc cagcagatcg ccatcatgat cggcgtcatc
     3901 tccagcgccc tggtcatcgg gctcatcgtc atcgccctca acaacgccta cacgaccgtg
     3961 acccccgccc gccaccccga ctacctcgcg gccgccgctc ccggcgccga gacgcagcag
     4021 ggtccggacg gcgcgatgta ccgcgtgcac tatgtcaacg agccggtcgg gaccgtcctg
     4081 atcggcaagt acctggtcga cgattcgggg cggatcgcct acctcgtcga tcccggcatc
     4141 gcgggcgtcg tgtcggaggt cgacggcaag caggtgacga agctcgattc gcccaaggcg
     4201 cggctcttca gtctcatcat cgacggcatc ctcacgcaga aacttccctg ggggctggtg
     4261 ctgatcggcg tgttcttggc gctcatgatg gagctggtgg ggatctcgtc ccttcccttc
     4321 gcggtcggcc tctatctccc gctgtcgagt tcggcaccga tcatggcggg cggtctcatc
     4381 cgcggcattg tcgaccgcaa gcgcaagagc aattccgcgg aggcggagtt ctcgccgggc
     4441 gtgctcatgt cgtccggatt catcgccggc gccgccatca tgggcatcgt cgtcgccggc
     4501 ctcaccggct tcggactgga caaggcgatc aacggctccg ggtggtcgca ctacctctcg
     4561 gaggcggact ggttctcgct gatcccgttc ggggcgctca tgtacatgct gtaccgggtg
     4621 ggcgtgtcgg tgaaggacaa ggggaaacag aaaagcgccg cctgagccgg cgcagcccga
     4681 cggcaaatga aaaggcggcc cgcaggcgta tccggcgggc cgcgctgttt gtcccggggg
     4741 ggccgcttat cggcggggcg ggggagaccc cggacgccgg aaatcgggcg cggccctcgg
     4801 ccggccgccc tacgtcagct cgccggggcg gcgctgggtg cgcaggagtc cccgggcggc
     4861 caggtcctcc tgctgtttga tcaggccgcg caccatgccg tacctcggcc ggactatctg
     4921 gaacgcgaga aacgagagga ccacgaacac cagcccctcg cggaaccgcc cggtgagcag
     4981 gaaataggcg aacccgttga gggcgatgcc ggcgatgagc aggaaaaccg gccgcaggcg
     5041 cacccggagt tcgccgcgga tatcctcctc gagcgtctcg atccgccgca ccatgggcaa
     5101 agccagtgtc ttcgaccgcc acaggacggc ggcggccccc tcggcgacgc tcaccaccgc
     5161 caggacatag aagagcgggt tggcggccgc cccgagcgcg ttgcccaggc cgccgcgctg
     5221 ctcgatgaca taggccgcca gcagcagccc ggcgggcagc aggatgttga tgaacagccc
     5281 ctggtagagc gacccggcga tcatgcgcgc cagatcgtac cgatcttcgt tcccttcgtc
     5341 tctcataacc gctccgcccc ttcgataaac tgttcgtatt tctcccgccg cggccaatag
     5401 aggccgagcc acacgagtcc gaccaggtag aaaatgagca tccgcatcag atcccaggtc
     5461 agaaggtaca cgacaaaccc gtagaggaac gttgcctcga cgaaggccat gcggaggacg
     5521 gcgagatgga agaacatccg gtcggccgtg cgccggccgg gccggcggcg ccagtcggcc
     5581 agcaggggcc gctcgatcag cagcgccagc agcggatcga cgatccccag gatcagcagg
     5641 aggtagaacg cgacgtcaat cgcccccgcg ccgttttcgc cgagcggcga cgaatccggg
     5701 tggacgagcg cggcaatggc gaagaagaac gccggcgcga caatcccgac cgctgccgcg
     5761 accacccgaa tgaccatcca cgtccgctcc tgcgtctcgc tcagcggttt cacgtcactt
     5821 cttctccctt ggcttggcgg aggcgctgga aaaacgcccg catcagttcc gcggccgcgt
     5881 cggccaggac gccttcgaca atttccgccc ggtggttcag ccggggatcg ccggggatca
     5941 ggaaaatcga accgcaggcg ccgaatttgg ggtcgcgcga tccgaacacg attcgttcga
     6001 tccgggacag tacggcggcc ccggcgcaca tggcgcacgg ctcgagcgtg cagtacagct
     6061 cgcagccttc gaggcgccag tcgccgagcg cgttggccgc cgccgtcagc gcaatcatct
     6121 ccgcgtgcgc cgtggcgtca tgcagatgga cggtgcggtt ggccccccgg ccgatcacgc
     6181 ggtccccctg caccaccacc gcgcccaccg gcacctcccc ctcctcgaaa gcctgctccg
     6241 cctcgcgcag cgccagccgc atatatatgc tgtaccggtc cgagtccatc cgaataatat
     6301 agatccgttg gattacccgc ggcaacccct ttgtcggccg accggggggg aggcggcccg
     6361 gcgcgacgcc gtcacttcag cagccgtcgg gtgatttcgt cggcgaggtg aatgccctct
     6421 tccgacagac gcagcatccc ccgttcggga atcagatgcc ccgactcgac caggagcgcg
     6481 tactgctccg tatcgagccg cgattcgata ggctggccga agcgttcggc gaaggcggag
     6541 cggctgatcc cgcgggccgt gcgcagaccg agcatgatcg cctcggtcat ccgctccggc
     6601 gcccccgact cgtcaaccgt gcgggggaga atgccggcct ggagcgcctt gatgtactcg
     6661 cgcacgttcg gccagttggc gaaccgggtg ttgtcgaggt aggaatgggc cgagggcccc
     6721 agcccgaggt actccgcccc ctcccagtag ccgaggttgt gccggcactc gaaccccggt
     6781 cgggcgaacg aggagacctc gtaccgcagg tacccgaggt ccgccatctg ttccacgccc
     6841 ccgcggtaaa gcgccagcaa cagttcctgg tcgggcatcc gcagtttccc cgccttcacc
     6901 cgccgccaca ggggcgtccc ctcttccacc gtgagctggt agtacgacac gtgggggggt
     6961 tcgaaatcga gcagttggtc gagatcggcc gccagcatct tgctggtctg gccggggagt
     7021 ccgaagatga aatcaactcc ccaggcgcgg aaccccagcg cgttggcgta gtaaatcgcc
     7081 cggtagctgt cgtgcgggtg gtggcgccgg tcgagcagtt tcagtagccg cgtgttgaac
     7141 gactgcactc cgaacaccgg ccggttcacg cccagccctt tgagccgctc gagctgctcc
     7201 cgcgtcaccg cctcggggtt gcactcgatg gtaaactcga tttcgtcggc gacatcgata
     7261 tgccgccgca ggtggccgag ccagtcggtc aggagatccg gatcggagat attgggcgtg
     7321 ccaccgccga taaagatggt cgtgacctgg ctgcgctgca ccgccgggtg ctcggcggcg
     7381 agttccgttt caatgcgcag cgcgtggtag aactcgcgtt ccagggcggc ctggtgcttc
     7441 tctttgtaaa agtcgcagta ggagcacgtg ttgcggcaaa aggggaaatg tacgtacagg
     7501 ccgaacggca tgcctcagag cgccagtccg acacggtccc agcgaacgtg ggacgggaag
     7561 ttgaagacaa catacccgat ccactggacc gcatcgatca cgtagggaaa tgagaatccc
     7621 ggcgccccgt cgtccggctg ccacgaccag tacacgaaca ccgcctttcc cttgacattc
     7681 tgggcgggca cgccccccca gaaccggctg tcccggctgt cgtcacggtt gtcccccatg
     7741 acgaagtact ggttctccgg caccacgtag ggcccgaaat tgtcgcggaa cgacagatcg
     7801 gcgggcagta tccgctgatc cgtgtgctgg gcgtgggccg gaatctcggc caccgcgccg
     7861 tccacataca gaaccttgtc gcccaccacg acttcctgcc ccggcagcgc cacaatgcgc
     7921 ttgatgtaat ccttggtggg attgttgggg taggcgaaga cgatgatgtc gccgaccttc
     7981 ggcgctccgc catacttgta ggccagcttg ttgacgaaga tgtaatcccc ttcgtacaac
     8041 gtcccctcca tcgaggagga gctgacccgg taggccgaga ccacgaacat ccgcagaagg
     8101 acggcggcca ggaaggcgag gatggcggtg tcaaggtact cgcgccagac cgggcgcggg
     8161 gcccggtagc ggcgcacccg ctcggcctct ttgtgagctt ccgcgcgctt gatgtcctgt
     8221 atgaggaaat cctcttccat acgtattagg gttatcggtt gcgcaccgaa cttgctttag
     8281 ccgcccacac ggccaaagtc cgcataaata tatggaccgg ccggaattac gccagccccc
     8341 gttttcccgc acgtcgtcgg ccggcgaaaa cgcattgaca gaccgcaact tcccgcctat
     8401 actataattg cgtccgccga ctggcccgcg ccggccggct cgcggcgagc cgggcttcac
     8461 cggcgggcac accaaggggt cttcgggtcc atgcggaagt cggcgcccat gaaacagtcg
     8521 ttcccgcgtt tgtagggtcg ccttggtcca ttacgtcagt gaaggaggta aggtgcacag
     8581 gctccgcatc gcgtcgtggc tggtgctggg gttgtccttg ctcggcccgg ccgagcaggt
     8641 tcgggcgcag aactgcgatt ccgtcccctt ctcccgcgac gaggtcggcc tccagacgat
     8701 cgtcggcctg cccggcgaca ccatctgggc ccccgtctgg gccatcaccg actccaccct
     8761 gatcgccttc cggatggtca ttcagtttga ctcgacaatt ctccggcccc tcttcgcggc
     8821 ccccggcagc gatcaactgg tcgtcgaacc ggccggccgc ttcgccggct cggtcaacct
     8881 ggcggcgagt ttgtccgagg ccgagattga ctccggcgcc atcgccgtcg tcttcctgcc
     8941 gtttctgaac tccaacgaca ccattccggc cggccgtggc gtcatcctcc gcattccttt
     9001 cgtcgttcag caggaggccc tgttcgcgga atactcgctc attagatttc cgcggcgcac
     9061 ggtctacttg gtcgactctt caagcgcgcc gcccgactcc acggcgatct gccgggggac
     9121 cgacttcgtg gagcggtccg gcgggccctc gggcatggcc gaaggatttc cccggccgag
     9181 cacggcgtac ttcttcaccg accccgaccc ggccggtcct ccgcgcatcg agtacttcgg
     9241 catcgacccg cgctcggcct ggcaggggga caccgtgcag gccgtctggt cggtgcgcaa
     9301 cgctgacagc atcgtgctgg cgccggcggt cggcatgcac ccgggcagcg ttcagaccga
     9361 gccgtttgag gtggtggagg gcgcgccgac gacctacacc ctgaccgcct tcggccgcgg
     9421 cgcccccgtc tcgcggacgc tgcgcctgct ggccgtcccc ccgggcgcca accgctttcc
     9481 atactggcag tactaccccg agccggtcgg cgggccggtg ctctctccca tccgccggta
     9541 cctttgggcg atcgatcccg atggcgacga gccgactttc tcgtcgccca atcttccgcc
     9601 cggtgcggtg ctcatcgccc ttggcggcgg cggcgtcctg ttcgagtgga cgccctcggc
     9661 cgccgatacc ggctatcgcg cggtgtcgat tctcacccac gatgcactcg actcggcccg
     9721 ctgcgactcc ctgaatctcg agatctatgt tcgggacgcc aacttccccc cgacctactc
     9781 ctatgacagc agcgaccgga tgatcttcga gggagacacc ctccgcatcc cgatcgccgc
     9841 ggctgatccg aacggaacca tcccctcgat cgcagcccac cttcactatg ccgatacgct
     9901 ggcggcgaac atgtcgttcg tcgacagcgg caacggcgcc ggcctcctga ctttccttcc
     9961 gagttatttc cagggaaacc tggcgccgac ctactatctg gtcagtttca cgattcgcga
    10021 cgcggtcgac ccggcgctca cggtcgccac cgcgcccgtg cgcatctacg tgcagaaccg
    10081 caacagcggc ctcggcccgc cggtgctctc gctctcggcg ggggaggggc cgtttaccgt
    10141 cgctgaggaa gacagcctgg tattcgaact gacggcggcg acttccaccg gcgacatccc
    10201 ggtgatctcg gcgagcgcgc tcccgccgac cgcctcgctc accgactacc ccggcgcgac
    10261 cgccggcgat cacaagattt tccgattcaa gccggccgac ggcatggtcg gggagtaccg
    10321 ggtcactttc tacgcggcca acgtcgacct gatcgattcg gccgcggtcg acctcgcggt
    10381 ggtcgcccgc aaccatccgc cggtcatcat gctgctgccg ggcaatgatg tgatcttcga
    10441 ggaagacacg gcccgtgtgc gcgtcctggc gttcgatccc gattcggtgc cgtccgtgct
    10501 caccgcgtac ctcgacggga ccgacacgct cgcgcgcaac atgacgatgg tcgattccgg
    10561 caacggggcg ggggtcctca ctttcgtgcc caaccgcgtg caggggagtc cctgcggccc
    10621 cgtgttctac tacctgcgct ttcgcgccac cgatccgatc ccgccccacc cctccgtcat
    10681 cagttccgtc cggaccatca gggtgtacga ctccggcctg ccctgctgta tcgggcggcg
    10741 cggcgacgtc aactacgaca gcaacgggtt gggagcgccg acggtggcgg atctcaccgg
    10801 cctgattgcg ttcgtgtttc gtcccggcgc cccgttaccg tgctaccagg aaggggattt
    10861 cgattcttcg ggcgcaatcg gcgtggtcga cattacgacg ctgatcggct acctcttcca
    10921 gggcggcccg ccgccgaccg agtgcccggc ggcgcgctcg cccggcgact ccgggccggg
    10981 gtgccgcgag tgaccggcac atccgccggc ggccgcaccg tcattcgcgg cggccggcgc
    11041 tgacatagac ctgatggaga aagggactca ccatgtatcc ttgctgctca ccgcggcgcg
    11101 cggcgatcgt ggcggccctg ctcgtcgccg tactgccggc agccccgctc ggtcccccgg
    11161 cggcggccgc cggggtcggc tcggccgaac gcggctcgaa agcgttctcc gcccaaccga
    11221 tcttcacctt cgacacccag tggcgggtcg aacacatcga gaccgctgat ctgaacggcg
    11281 acggtatccc cgacatcatc gccggcgaac agaacaccga ctactacggc gacccgcaca
    11341 acgtcttcgc cgtcgacggc gccaccggcg ataccctctg gcggtatccg cttcaggatg
    11401 cggtgcgctc gatgaccatc ggcgacgtca acaacgacgg cgtgatggac gtcatcgcgg
    11461 gcgcctcgta caacgcctcg aacacccccg acggccgcgt ccacgccatc aatggcgaga
    11521 acggcgcccc cctctggacc taccccgtga acaacaccgt ctcctgcgtc gccgtcggag
    11581 atttcgacgg tgacgcgttt gccgacgtcg tcgccgggtc gtttgacgac aacatctacg
    11641 ccatcgccgg tgtcgatggt tcctacctct ggagcgtcac catcggctcc ctctggatca
    11701 acgacgtggc cgccgccgac gtcaacggcg acgggatcga cgacgtcgcc tacgcccacg
    11761 aatacctcgc cggctacacc aaccacatcg gggtgcgcga cgggatcgac ggctccgtga
    11821 tctgggacct gccggtcgcc tacgtgccga cgcgcgtgct ctacggcgac ttcgacgccg
    11881 acgggacgct cgatctcgcg atcggcgcca tggacgagct cgaccagggt tgggtcttcc
    11941 tgcgctcggc cgcctccggt ggactcatct gggactacca gatcttcagc gtcgagcacg
    12001 tcaacggtct cgtcaccctc ttctcgtggg atctcgacgg caacggcgac caggacctgg
    12061 tcgtcggaaa ctacctcggc gcctaccacg tgtatgcgtt cgatggtccg acggcgagcc
    12121 tcatgttcac ctccgagccg ctcaccagct tcccgcagga actggccttc ggcgacgtga
    12181 ccggtgacgg cgcgctcgac atcgtctgct ccgcctggga ccgggtcgag gtgctcggcg
    12241 cggacgacgg caagaagctc tggtactact cggtggccgg cctgatcaag gacgtggccg
    12301 tgggcgactt cgacagcgac ggcgtgctcg acgtggccgc cggcggcggc gccgaatatg
    12361 tcggcaccaa tcccggcaaa ggcgtctggg cgctccgcac cgtctactcc ccgctcctct
    12421 gggagtacgt gttcaaccag tacggcaacg aactgacaat ctgcaatctc aacgacgacg
    12481 cctacctgga tgtcgtggcg gtcatgtcgc tgggcgatgc ggcgctggcg atcaacggcc
    12541 tcaccgggca ggagctctgg acctgggtcg ggacggagaa cctttacgcc gtcaccagcg
    12601 gcgacttcaa cagcgacggc gtcgacgagg tcgccgtggc cggcgccgac aagcgggtga
    12661 ccgccctcgg ggtcgtcgac aaagcggtgc agtggcagtt cacgacgccc accgcccaga
    12721 tctaccgcaa gtgccttgcg tcgagcgatc tcaacggcga cggtgccgac gatgtcatcg
    12781 ccggctgcga cgacggccat gtctacgcca tcaacggcct caccgggatc gccctctggg
    12841 acgccccgag cctcggcgcg gtcaacgaag tggaactggc gcagatgaac gacgtcggcc
    12901 cgctcgatgt cgtcgccgcc tcctcgaccg gcaaagtctg ggtgctcaac ggcgagaccg
    12961 gcgccgtcct ctgggattac accaccggcg gcacctgcga gcacgtcgag gtgctcgacg
    13021 tcaacggcga caaggtcaac gacatcgcgg ccggcgtcgc cccgagcgcc gccagggtga
    13081 tcatgatcaa cggcgccacc catctcgaaa tgtggaacgt gagcctcggc ctggcctcca
    13141 acacccacag cttcgcccac ggcgacctga gcggcgacgg actggacgat ctcgtcgccc
    13201 ccggcaactc gaccgaccgc aaggtccacg ccctctgggg actcaccggc ggcgacctct
    13261 ggacgccctt cctgaccggc gacgaggtca actgcgtctg ggtgcaggac atggacggcg
    13321 acggggtcaa cgacgtcatc gccggctccg acgaccagct cgtgtacgtg ctgcacggca
    13381 acaccggtgt cccgtactgg cagttctcca ccagcggcga cgtcatgcac ctcatggtcg
    13441 gcgacatcgc cggccccggc cgaccgagca tcgcctgcgt gacgttcgac ggcgacggca
    13501 cggtctacgc cttcgattcc tggtacgtcg agccgggctg ctgcacgctg cgcggcgact
    13561 acgacagcga cggcgcgatc aaagtcgccg acctcaccca gctcgtcgcc tacctcttcc
    13621 ggggcggccc cgcgccgggc tgttttgagc acggcgatat cgacggcgac gggacgatca
    13681 aggtgagcga tttgaccctg ctgatctcgt acctgttccg cagcggcccg ccgcccgccg
    13741 cctgcccgta agtccgggct gtctcggcca agccgagaga aagacgacgc ctgccgcttc
    13801 cccccgcgcg ggggaagcgg cacggccgcg gcctcgccga aaaaaataga cccccgatcc
    13861 gcaatatcgc ttgacaggca atcccctgct gcctaaatta taagtgaaac cgtcagggtc
    13921 ttttcgttca ccgccaagag aagtccatat cctcacctcg tgcgacgcct gtggtgcgtc
    13981 tcggactgga gagaaaagcc aattccccgc ctcgcatgcc tcttcatcgg aacgggtaat
    14041 gctcaacgtc agtgaggagg tgcatatgag atgaatgtgt ccgcgacggc ggctggtcgc
    14101 cgcgccgcgg acccaggaga gatcctgcca agaccagaga gacctactac cgcgaaagcg
    14161 aaacgattga ccgcaatgtc ccggcgtctc ctcgggcatc agggatagat gccggctcgg
    14221 tgccggtccg gaaaaacggc ggcccaccca acatccgccc atcatccccg gatcggtgcg
    14281 gcgggagcgg cggcgggaag cggttgcgat cgtttcacaa tggaaacgga tctccgtgac
    14341 acgaagcccg gcggccggac aaccggccgc cggtgcttta ttcccctact ggctaagggg
    14401 atccccccat actccgtccc cggccggcga accgcgcgcc ccgcatcgac actcccgccc
    14461 ctctgctaat ccgttcagaa ccatcggttt gcccggcgcc aagcgtgtcc cggccgcctc
    14521 cggcaccgcg cttgcactac cccagcgcga acagactggg aacgaactac tacggcggac
    14581 agtacgtatg agagtcaagt tcgtgctgac gatggacaac ctggtcgtcg atgataagag
    14641 catcgaccag gtggtgctcg attgggaggc caatgtcgac caatcggaaa tcctccagtt
    14701 gtcccaccag tggataacct cgaggaactt cctcactcag cgcatgttgg gactagtgca
    14761 ggttggtgag tcctccctga ccattgaacc gctcgaagag gacgattatg cacagtggtg
    14821 agtggctggc acaggcccgc gcgctgctcg aacgggctgt ccaggccgtc caggatctcg
    14881 ccgcggccgc ggccggggcg caggagtgga tcgtggcgca ctttggccag aacggcctct
    14941 acgccgcggc ctttatcggt gccgccctcg gcctttacct gatcctgcag ctgatccgca
    15001 tcaccctggc cgctctcaaa tacctggtcg tcccgggcgt cggactcgcc ctgctcggct
    15061 cgctggtgct gccgttctcg ttcttctttc tcctgccgat taccctgtcc gcctgctcgc
    15121 tcgtgctgct gtttaagggc tgacgccaca cctgtgggag gccggcccct cccttggccg
    15181 gccgattccc cgcccgcggt tccccatggc atgctaaccg cctgaggttc aggcggtttt
    15241 cttctgcccg ccgcccggca aaccgtttgc ccccgtttca tctgaaacaa agatccgagc
    15301 tccgctcggc cgacccg
//
LOCUS       DAOPOE010000078        15187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4644_length_15187_cov_4.481034, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000078 DAOPOE010000000
VERSION     DAOPOE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15187
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_15187_cov_4.481034"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKM94_10325"
     CDS             <1..594
                     /locus_tag="PKM94_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD67069.1"
                     /translation="ARVFLAMLIGEENMDDGARMGEWAKRGGVDAVRFCGYTPAGRGR
                     RRDVKSRLGFTDVQRLLEFAETMEKDASMVIDFDVAWGPVPPTFGFHQCTAGVETFYL
                     KGNGDIYPCTALSHRRFRVGNIRQRPLPEIWHSPEMSAMSRYPRERVKEPCRSCDNFS
                     ACRGACRAGAFVHTGDLDAAHPLCLYLAARERAAVRR"
     gene            956..1888
                     /locus_tag="PKM94_10330"
     CDS             956..1888
                     /locus_tag="PKM94_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67070.1"
                     /translation="MRGNSQIAGALAAVVTMAAAVVSLSWTAAAVAEEGAGSAPSFQE
                     VRLAASDVTPIEKDGCPTVYYYDEESTAYVVWRLPQEGVEEFGERFDQEGVSVCTLVT
                     VRALLYGPQMAGSPDVEVVVYQDDGTGLPGAQLAAVTVPNASLPTTGLGWIEADFSPS
                     SLVFSEGETYHLTLRVSPAVAGDTVSLCSDEATGPHSGENRSEYFDGDAWWTLSSAVG
                     TDYVFVFEAEIDFGCDTTCCVGGAGNVNGDPVDKVDVADLTFPVSYLFRGGVEPPCRE
                     EANVNGDPDGDVSVSDLTLLVGYLFRGGPAPAPC"
     gene            2147..2641
                     /locus_tag="PKM94_10335"
     CDS             2147..2641
                     /locus_tag="PKM94_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOD67071.1"
                     /translation="MDVIEQLGSLALATRLRRLADRLQRDVSQVYRELNVEFEARWFA
                     VVYYLKDHNEAPLTTVATDLGLTHPAINQVAGQLTRRGLLRSTRDPGDYRRRLLSLTD
                     RGRETVRALEPVWAVIADASTELGSTDGSDILEALRRLERQLDETSMYERVMTRLRRE
                     GILP"
     gene            2683..4026
                     /locus_tag="PKM94_10340"
     CDS             2683..4026
                     /locus_tag="PKM94_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD67072.1"
                     /translation="MKFQALWAAMMWAAVAVFAGGPEAGAQADPDDTGPAIDRTVKAE
                     IIDSVTAALDRYYVFPDVAKKMGEHARRQLKKGAYDTLATVGTFARGLSEDLLTVCHD
                     RHFGLRYVPRAQLEAMLDTINRPTAAEELRRARLDNFGFRKAEQLPGNVGYLKLDGFS
                     GLPEARATAVGAMHFLAHTDALIIDLRDNGGGSPDMIQILTSYFFKDQVHLNTFYVRE
                     TDSLQQFWTTSYVDGEKMADVPLYVLTSGGTFSAAEEFTYNLKNLKRATVVGETTGGG
                     AHPVATHGFPNLEVGVRVPFGRAINPISGTNWEGTGIAPDIACAADRALEVALLEAYR
                     KLKAECGDEQLLATYDWQIDGLEAQRNPVTLEAAALQEYVGDFGPRRIALENGALVYQ
                     REGNPKFRMTPMGGDLFMFADIPYFRLKFVRDAGGGIVAVEGHYEGGQVDRNERT"
     gene            4270..6213
                     /locus_tag="PKM94_10345"
     CDS             4270..6213
                     /locus_tag="PKM94_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOD67073.1"
                     /translation="MRRAMLVTAAVWLALCGSAAGAGPEYGEIAEAEWQIGPPADYPE
                     ANAVVIFDRVYRSMTIPQGEFSPQLELRRHVRMKAFNKAGAEEIGNGSFGHSEKDHVS
                     DLCAHTVTPDGQVFKVNDKDFLTRKAGSTTIVTFAFPQADSGCILEFTYKVWGGSYIR
                     WETIPFSHEVYTLETEYVLEVPVGPQYQYRAHNLPAGTGNPVVEKLPGTDMKAKKRFT
                     WRVQNVLPVQDEPYMGFERDYVPAVSLVLSDLSRKATVADAVEQWSTIGQLWETAVVD
                     EYCSEPRDFKKRLREIVKGLKTDYDKSKALYSFAVSTIETRQDGFDDGFHHDNLAGLW
                     REKFGTAGEKNLLLLAMLRSERIRAWPIMICTRDVAAFEPTWVDPLQFSHMIIFAEVE
                     QGGIYLDATTKYNAYGTLPPVCRTDIGLLLDGDKSELVKVVTNDPPSSRIDLIVYRMD
                     AEGNAACSTSALFTGYLCSNYGRRYETREANDFLDRDILNVDAPGYRRGSFEATLDSL
                     GRLRVMTGYAVEGAGRRLDDHLAVNCPATMFSRNPFESERRVFPVNFNHAFTYQAIAR
                     IVGPEGSTVSQLPPDTSFAYEGLLYERQSSSEGQAAQVHSKFTVSRPLFEVDEYMMVR
                     LFFQRVESALGEQVVFTTAKEGL"
     gene            6210..8090
                     /locus_tag="PKM94_10350"
     CDS             6210..8090
                     /locus_tag="PKM94_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOD67074.1"
                     /translation="MNRRVWGFLAAGLLGWSDAAAAPAEADDRGGYFENRQWVTEVEA
                     NGLRHRVRARLVILNARGDKFAGVAVSGDQFNQIEDFRASVYRADGKKVYERKLKDMF
                     RTCGFGPSYALYSSNCTYSLDLPGPAYPYTIEYEYAVRSKSLFFLRGAAMQSYLPVVR
                     ALCQVTAPSEPPLHYKVYGLAITPRVVRTGEKTLYEFAAGNLAPLPNESQLPPEAEYP
                     GRVAFSPHAFRLENSAYSGFSWREVGNWYRTLAADRYLAWKAGVTAGDVRQMAREAYE
                     RVTGNTRYVAVSIGIGGWQPHAAAETQKLGYGDCKDMTTLLVSYLRQAGVAAYPALIS
                     TRGATPIDPDFPTFDFNHVITAALIDGDTLWMDPTCDLCPFGQLPLGDQGMPVLLVTD
                     SGGVLVRTPAAKAEDNVWARRTHLAIGADYRVNLSAEWELCGVPALWLEAMFTASDSE
                     DRRLMVGQLLTGDNGGYRVTEASLTRRPADSGGGVAVRVAAVSERPVDLVKGTAYVQP
                     AVYRDFAEVRALDFATRRYPVDLEVPQLMTDEVTVTWPSMGTATAEGPGADSIACGHG
                     FWRRTAAAADSAAVRLTMEAACLGSVVEVGELPTFAEYLKKRQAREREPIRLTASGAA
                     GR"
     gene            complement(8224..9426)
                     /locus_tag="PKM94_10355"
     CDS             complement(8224..9426)
                     /locus_tag="PKM94_10355"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOD67075.1"
                     /translation="MRKKITSLALTGLLAALVGCSDRSPVGSASTADQPFALAAGVIV
                     PDSVKTAALSVYCSGPSLQTVNVYRVTAPWAESTVTWNSFAGAYDTAAVHGSFAAAAP
                     GWKTAEVTALVRQWVGGAAPNCGLLLGQTTNLFPRGMFFSRENAFMQPYLTVTYGGPT
                     GDTSVQFPVLRDAAIDESVPNNPLGLAQLLFTGWAADGGLKNQSLLLFDLPVYNPPPP
                     DGDSTGIDEPNDTTITDPPDTTIIEPGDPDDQGCTRPWPWWLRHSGCAKGSESDEISA
                     LLPLWVGTPGGAHSVEIDSNCKAAQSLQRRNHDRTFPPVKPLLTELLAAKLNIAAGAD
                     DSAVAGVIAAADAYLATHPVDSRRSVDRRAFKQIHEWYRALHAFNVGDIGPGACAPDA
                     DTSVNPRR"
     gene            complement(9735..10961)
                     /locus_tag="PKM94_10360"
     CDS             complement(9735..10961)
                     /locus_tag="PKM94_10360"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOD67076.1"
                     /translation="MKILVLNAGSSSVKYQLIDSETEKALAKGMVSRIGMSGSLLAHK
                     PFDRPEVKVSGEILDHIQAVEYIVTMLLSPNHGVIKDRAEIGAVGHRVVHGGEKFTDS
                     VLITPALMQELRNLIELAPLHNPHNIRGITACERTLPGLPQVAVFDTAFHYTMPGYAY
                     IYGIPYVLYKRYGIRRYGFHGTSHKFVSDRAAVMLRRPLAELRVVTAHLGNGASITAV
                     DRGVSVDTSMGFTPLEGLLMGTRSGDMDPAIILHLMAREELSLHEANTLLNKHSGLIG
                     ISGVSSDMREIQSASQAGNANAQLALDIYCYRIKKYVGAYAAAMGGIDALIFTGGVGE
                     NSAVVRAKVCEGLAFLGLQVDPEKNSAAHAAETDISSAGAGGHVLVVPTNEELVIARD
                     TLRIVTERRGQSARVK"
     gene            complement(10974..11342)
                     /locus_tag="PKM94_10365"
     CDS             complement(10974..11342)
                     /locus_tag="PKM94_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOD67077.1"
                     /translation="MTVNEYPGYVTTRIIVPLLNEAMHVLMEGVASADDIDRAMKLGF
                     GFNMGPLALADVMGLDVVMKWMENLLDELAEHKYNPCPLLRKMVRSGHLGVKTGRGFF
                     VYDNEGNRVPNPAAAAAVTK"
     gene            complement(11340..11906)
                     /locus_tag="PKM94_10370"
                     /pseudo
     CDS             complement(11340..11906)
                     /locus_tag="PKM94_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            12132..12794
                     /locus_tag="PKM94_10375"
     CDS             12132..12794
                     /locus_tag="PKM94_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD67078.1"
                     /translation="MKQAIPFILLLLLAIGLIAAQPAEAAIQRVAARYNMVDVYGGYA
                     SPHGEYGEVGLVMFIDEFDRPVEVDADQVFDAGWSLGFNYGTLYADRFLMQLGFRYTN
                     HQEQDWFKDHPDEFHLAQYDLNLNLHVYAVPPTKSIVAPYGGVGVQAGLLNIEIAGYD
                     DESDLTILMAADFGLDLKVAEFSKGRSFLTLASINSYDLFGTEDRPKYLTIGGGLRYW
                     FR"
     gene            12871..14496
                     /locus_tag="PKM94_10380"
     CDS             12871..14496
                     /locus_tag="PKM94_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67079.1"
                     /translation="MRGRAARAAVAFGLAAVMAAGGCGSDSPNGPQPQGGQVVALNGV
                     RFQGFMGSDSTTTAAQFAVTDAGGNRLANHQIQVAPVRGDGAAPRSIATDAAGVAVLQ
                     YDFSGRMGHAALRLYAEGTDTLEVYFRADAIIPGVHGQVGYVLFDDDYAEVKRWLGQP
                     ESEDVLPNRSPVYASWESTRGLVVMLYDWDLDGKLYDSSSVYGAIAVTGFAGTTPDGD
                     AVGMGSTIAEVRKRFGPPTDWGVYPEEPNTLQYYFGLWDLYVFADGSQPDTPAVQMTL
                     GEYIPAFPTEIVPLNGLLYRVANLPGGMTYPALQFAVEDSSGARIGNVDIQLSHAAGA
                     GSFLSTGGGSISYVTTATTPPGLAEFRYLMTPGLSGSAVGRLTIPVIDTLDIYLRRNV
                     LAPGSGGQGQYVLFNETYAEAAAWNGTAASVAGDGRFGPTYAYAAYPDSGLTFVFRDP
                     GGTNQVTSSSQVYGVIAEAPWPGTMASASAAQRGIRIGSTYAEVTGAYGAPSSSATAG
                     DTLGLVYDGYPVRFELVPAVDGAVVRILTWEGP"
     gene            14616..>15187
                     /locus_tag="PKM94_10385"
     CDS             14616..>15187
                     /locus_tag="PKM94_10385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD67080.1"
                     /translation="MRFMTSVLMIVAAAFVLVIGCSSNDAQSADNTAQASGKTTAQAP
                     AAKPAAATTGNPYYDSLAQMTHPIRDEKNPIVTIRTDFGDMVCELYRDVAPAHADSFV
                     ALTKKGFYNGLTFHRIIDGFMIQGGDPKGDGTGGPGYSLTAEFSELPHADGTLSMARS
                     NDPNSAGSQFFVCLGRAAFLDRKYTVFGQVL"
BASE COUNT         2551 a         4208 c         5749 g         2679 t
ORIGIN      
        1 gcgcgggtgt ttctcgccat gctgatcggg gaggagaata tggacgacgg cgcgcggatg
       61 ggcgagtggg ccaagcgggg gggggtcgac gccgtgcggt tctgcggcta cacgccggcc
      121 gggcgggggc ggcgccgcga tgtgaagagc cggctggggt tcaccgatgt gcagcgcctg
      181 ctcgagttcg ccgagacgat ggagaaggat gcgtcgatgg tgattgattt cgacgtggcc
      241 tgggggccgg tgccgccgac gttcgggttc caccagtgca cggccggcgt ggagacgttc
      301 tacctgaagg ggaacggcga catctacccg tgcacggcgc tctcgcaccg gcggttccgg
      361 gtgggcaata tccggcaacg gcccctgccg gagatctggc attcgccgga gatgtcggcg
      421 atgtcgcggt atccgcggga gcgggtgaaa gagccctgcc gatcgtgcga caatttctca
      481 gcctgccggg gggcgtgccg ggcgggggcc ttcgtccaca ccggcgatct cgacgcggcg
      541 cacccgctgt gtctgtacct ggcggcgcgg gagcgggccg cggtgcggcg atagggacgg
      601 aagagagcca cagaaggcgc ttgacaatag agacttgccg cgatatcgtc aagcaaggca
      661 aatcgagaca gctttatcgc tgcagcagac cctcgttgaa actcaacatc gtgtgacaac
      721 tgccttcagc cgggactccg aatgacatct cgggcagggt cgtctcccgg ccggaacatg
      781 atcggcggca aagccgacgt tggtcggaaa cagttggccc cttcagacga cgcagggaag
      841 ttcatcgtta cacctggagt ccgggaagat cgatgaggcg gacgttcgac ggacgttgcc
      901 cgagcgggtg acgaagtgtc ggtccgaagc ggttctcaac gagacgaggt gtcatgtgag
      961 gggtaactcg cagattgcgg gggctcttgc ggctgtcgtg accatggcgg ccgcggtggt
     1021 gagtctgtcg tggacagcgg cggctgtcgc tgaggaaggg gcaggttcgg ctccaagttt
     1081 tcaggaggtg cggctggcgg caagtgatgt gacgccgatc gagaaggatg gttgtcctac
     1141 ggtctactat tacgacgagg aatcaacggc gtatgtcgtc tggaggttgc cgcaagaggg
     1201 ggtcgaggag ttcggcgagc gattcgatca ggagggggtc tcggtctgta ctcttgtgac
     1261 ggtccgggcg ctgctgtatg ggccacagat ggcgggctcg ccggatgtgg aggtggtggt
     1321 gtatcaggat gacggtacgg ggctgccggg cgctcagctg gccgcggtca ccgttccgaa
     1381 cgcctccctg cccaccaccg gcctgggctg gatcgaggcc gatttttcgc cgagcagcct
     1441 ggttttcagc gagggagaga cctaccacct caccctgcgc gtttcgccgg cggtagccgg
     1501 agataccgta tcgctgtgca gcgatgaggc gaccggtcct cactcgggag agaaccggtc
     1561 agagtacttc gacggcgacg cgtggtggac gctcagctcg gccgttggaa cggactacgt
     1621 gttcgtattt gaagcggaga tagatttcgg ctgcgatact acctgctgcg tcggcggcgc
     1681 cgggaatgtg aacggggatc ccgtcgacaa agtcgacgtg gcggatctga cattccccgt
     1741 gtcgtacctg ttccgcgggg gagtggagcc gccgtgtcgg gaagaggcca acgtcaacgg
     1801 cgatcccgac ggtgatgtga gtgtttcgga cctaacgctt ctcgtcgggt acctcttccg
     1861 cggggggccg gcgccggcgc cgtgctagac cccggaccgc gcggagacac agcttcggaa
     1921 gtcgaaagag acgggcggcc gcccgcaatc ggacgatcgg cccttcctat ctcaagccgg
     1981 tggggtgggc gatcgaaaga acctttttcc ttattgcagt gcgaaacggg gagtggtaga
     2041 ttgagccatc ctgaaggcag gtcggtgcga tggtcatcgc acgcatccgc gaaacaatta
     2101 tgtaagcact tacgtacact cggatgatag agggagcccg aagacaatgg acgtgatcga
     2161 acaactcggc tcgctggcgt tggcgacccg gctgcgccga ctggcggacc gcctccagag
     2221 ggatgtgtca caggtgtacc gggagctcaa tgtcgagttt gaggcgcgct ggtttgcggt
     2281 cgtgtactac ctgaaggacc acaacgaggc gccgctgacg acggtggcga cggatctggg
     2341 gctgactcat ccggcgatca accaggtggc ggggcagttg acgcggcgcg gactgttgcg
     2401 gtccacccgc gaccccggcg actaccgtcg gcggctgctg agtctgaccg accgcgggcg
     2461 ggagacggtc agggcgctgg agccggtgtg ggcggtgatc gcggacgctt cgaccgagct
     2521 cgggagcacc gacgggagcg acatccttga ggcgctccgg cgtctggagc ggcagctcga
     2581 cgagacgagc atgtacgagc gcgtcatgac cagacttcgg cgggagggaa ttcttccgta
     2641 acggctcccg cacgaccaac atccgaagtg cggaggtgag cgatgaagtt tcaggcgttg
     2701 tgggctgcaa tgatgtgggc ggcggttgcc gtgttcgccg gcgggccgga ggccggggcg
     2761 caggcggatc ccgacgacac cgggccggcg atcgaccgga cggtcaaagc ggagatcatc
     2821 gacagcgtga cggcggcgct cgaccgctac tacgtcttcc cggacgtcgc gaagaagatg
     2881 ggggagcacg cgcgccggca gttgaagaag ggggcgtacg acacgctggc gacggtgggg
     2941 acgttcgcgc gggggctttc ggaggatctg ctgacggtgt gccacgatcg gcatttcggg
     3001 ctgcggtatg tcccgcgggc gcaactggag gcgatgctcg acacgatcaa ccgtcccacg
     3061 gcggccgagg agctgcgccg ggcgcggctg gacaatttcg ggttccgcaa agccgaacaa
     3121 ctgccgggca acgtggggta cctgaagctg gacggtttca gcggattgcc ggaagcgcgg
     3181 gcgacggcgg tcggggcgat gcattttctg gctcacaccg acgcgctcat tatcgacctg
     3241 cgcgacaacg gtgggggcag cccggatatg atccagattc tgacgagtta cttcttcaag
     3301 gaccaggtgc acctgaatac gttctatgta cgcgagacgg actcgctgca gcagttctgg
     3361 acgacgagct atgtcgacgg cgagaagatg gccgacgtcc ccctgtacgt gctcacctcg
     3421 gggggcacct tttcggcggc ggaagagttt acgtacaatc tgaagaatct gaagcgggcg
     3481 acggttgtcg gggagaccac gggcggtggg gcgcacccgg tggcgacgca cggatttccg
     3541 aacctcgagg tgggcgtgag ggtgccgttc gggcgggcga tcaacccgat ttcggggacg
     3601 aattgggaag ggacggggat cgcaccggac atcgcgtgcg cagccgaccg ggcgctcgag
     3661 gtcgcgcttc tggaggccta ccggaagctg aaagcggagt gcggggacga gcagttgctc
     3721 gcgacctacg attggcaaat cgacgggctg gaggcgcagc ggaacccggt gacgctcgag
     3781 gccgcggcgc tgcaggaata tgtcggcgat ttcggacccc ggcgcatcgc gctggagaac
     3841 ggcgcgctgg tgtaccagcg cgagggaaat ccgaaattcc ggatgacgcc aatgggcggg
     3901 gacctgttca tgtttgcgga catcccgtat ttccggctga agtttgtgcg ggatgcaggc
     3961 ggggggatcg tggcggtcga agggcattac gaaggggggc aggtggaccg gaacgaacgg
     4021 acgtgagggg ggcggcaagc agattggcag gccgccggga tttcccggcg gccttttttt
     4081 tgccgccggc gcggccgtaa aatgatcctt gactccggac tctccgttgc ctaggctatg
     4141 ggcgtgagtt gggcgtgacg accggagtgc gggatgcgga tcgcgccggc gcagggctgt
     4201 acggccgaga cggatctcca gagcgaacaa gggtaggacg caggcgaagg actcatgacg
     4261 gaggtttcca tgaggagagc gatgctggtg acggcggcgg tctggctggc gctgtgcggg
     4321 agtgcggccg gcgcgggacc ggagtacggg gagattgccg aagccgagtg gcagatcggt
     4381 cccccggcgg attatcccga ggcgaacgcg gtcgtgatct tcgaccgggt gtaccggtcg
     4441 atgacgatcc cgcagggcga gttctccccc cagctcgagc tgcggcggca cgtgcgcatg
     4501 aaagcgttca acaaggccgg ggcggaagag atcggtaatg ggtcgttcgg gcactcggag
     4561 aaggaccatg tcagcgacct ctgtgcgcac acggtgactc ccgacgggca ggtgttcaag
     4621 gtcaacgaca aggatttcct gacgaggaag gcgggctcga cgacaatcgt cacgtttgca
     4681 tttccgcagg cggacagcgg ctgtatcctc gagttcacct acaaggtgtg ggggggaagc
     4741 tacatccgct gggaaacgat tccgttcagc catgaggtgt acacgctgga gacggaatat
     4801 gtccttgagg tcccggtggg gccgcagtat caataccggg cgcacaacct gcctgcggga
     4861 acagggaatc ccgtggtcga gaaacttccc ggcaccgaca tgaaggccaa gaagcggttc
     4921 acgtggcggg tgcagaatgt tctgccggtg caagacgagc cctacatggg gttcgagcgg
     4981 gactatgtgc ccgcggtgag cctggttctc tccgacctgt cgcggaaggc gacggtagcc
     5041 gacgctgtcg agcagtggag cacgatcggg cagttgtggg aaacggcggt tgtcgatgag
     5101 tattgcagcg agccgcggga tttcaagaag aggttgcggg agatcgtgaa ggggctgaag
     5161 acggactatg acaaatcgaa agctctgtac agctttgccg tgagcacgat cgagacgcgg
     5221 caggacgggt tcgacgacgg tttccaccat gacaacctgg ccggcctgtg gcgggagaag
     5281 ttcggcaccg cgggcgagaa aaacctgctg ctgctggcga tgctccggtc ggagcggatc
     5341 cgggcctggc cgatcatgat ctgcacgcgg gacgtcgcgg cgttcgagcc cacttgggtc
     5401 gatccccttc agttctcgca catgatcatt ttcgccgagg tcgagcaggg gggaatttac
     5461 ctcgacgcca ccacgaagta caatgcctac ggcacgctgc cgccggtttg tcggacggac
     5521 atcggcctgc tgctggacgg ggacaaatcg gaactggtga aagtggtgac gaacgacccg
     5581 ccgtcgagcc gcatcgacct gatcgtctac cggatggacg ccgaggggaa cgccgcctgc
     5641 tccacctcgg cgcttttcac gggctatctg tgcagcaact acgggcgccg ctacgagacg
     5701 cgggaagcga acgacttttt ggaccgcgac attctcaatg tcgatgcgcc cggctaccgg
     5761 cgggggagtt ttgaggcgac gctggattcg ctggggcggc tgcgcgtcat gaccggctac
     5821 gcggtcgagg gggcggggcg gcggttggac gaccacctcg cggtgaactg cccggcgacc
     5881 atgttttccc gcaatccgtt cgagagcgaa cggcgcgtct tcccggtcaa tttcaaccac
     5941 gccttcacct accaggcgat cgcgcgcatt gtcgggccgg agggttccac ggtcagccag
     6001 ctccccccgg atacgtcctt cgcctacgaa ggtctcctct acgaacggca gtcgtcgtcg
     6061 gaagggcagg cggcgcaggt gcacagcaag ttcacggtga gccgcccgct cttcgaggtc
     6121 gacgagtaca tgatggtgcg gctgtttttc cagagagtgg agtccgcgct gggcgaacag
     6181 gtggtgttca cgacggccaa ggagggcttg tgaaccggcg ggtgtgggga tttctggcgg
     6241 cggggctgct gggatggagc gacgccgcag cggcgccggc ggaggccgac gaccggggcg
     6301 ggtatttcga gaaccggcag tgggtgaccg aggtggaggc caatgggctg cggcatcggg
     6361 taagggcgcg tctcgtcatc ctcaatgccc ggggcgacaa gttcgcgggg gtggcggtca
     6421 gcggcgacca gttcaatcag atcgaggatt tccgggcgag cgtttaccgg gcggacggga
     6481 agaaggttta tgagcggaag ttgaaggata tgttccggac ctgcggattc gggccgtcgt
     6541 acgcgctcta ctcctccaac tgcacgtact ccctcgacct tcccggcccg gcctacccct
     6601 atacgattga gtacgagtat gcggtgcgtt cgaagtcgct gtttttcctg cggggcgcgg
     6661 ccatgcagag ttacctgccg gtggtgcgtg cgctctgcca ggtgacggcg ccgagcgaac
     6721 cgccgctgca ctacaaggtg tacgggctgg ccataacgcc gcgggtggtg cggaccgggg
     6781 agaagacatt atacgagttt gcggcgggga atctggcgcc gctgccgaac gaatcgcagc
     6841 ttccgccgga ggccgagtac ccggggcggg tggcgttttc gccgcacgcc ttccgcctgg
     6901 agaacagcgc ctacagcggt ttctcgtggc gcgaagtggg gaactggtac cggacgctcg
     6961 cggccgaccg gtatctcgca tggaaggccg gggtgacggc gggcgatgtg cggcaaatgg
     7021 cgcgcgaggc ctatgagcgc gtcaccggga acacgcggta cgtggcggtg tcgatcggga
     7081 tcggcgggtg gcaaccccac gccgccgccg agacgcagaa gctcggctat ggcgactgca
     7141 aggacatgac gacgctgctg gtgtcgtacc tgcggcaggc gggggtggcg gcgtacccgg
     7201 cgctcatctc gacacgcggg gcaacgccca tcgatcccga tttccccact ttcgacttca
     7261 accacgtcat cacggcggcg ctgatcgacg gggacacgct gtggatggac ccgacctgcg
     7321 acctctgtcc cttcggccaa ctgccgttgg gtgaccaggg gatgccggtg cttttggtga
     7381 ccgacagcgg gggcgttctc gtgcggacgc cggcggcgaa ggcggaggac aacgtgtggg
     7441 cgcggcggac gcatctggcg atcggcgcgg actaccgggt gaatctcagc gccgagtggg
     7501 agctgtgcgg ggtcccggcg ctatggctgg aagcaatgtt cacagcgtcg gatagcgagg
     7561 atcggaggct gatggtgggg caactgctga ccggcgacaa cggcggctat cgggtgacgg
     7621 aggcgtcact gacaaggagg ccggcggact cggggggagg ggtcgcggtg cgcgtggcgg
     7681 cggtgtcgga gcggccggtc gacctggtca aggggacggc ctatgtgcag ccggcggtgt
     7741 accgggactt tgcggaagtg cgcgctctcg attttgcgac ccggcgctac ccggtggacc
     7801 tggaggttcc gcagctcatg acggatgagg tgacggtgac ctggccctcg atggggacgg
     7861 cgacggcgga agggccgggg gcggactcga tcgcctgcgg ccacgggttc tggcggcgga
     7921 cggccgccgc cgcggacagc gcggcggtgc ggctgacgat ggaggcagcc tgcttagggt
     7981 cggtggtcga ggtcggggag ctgccgacgt tcgcggagta tctgaagaag cggcaggcca
     8041 gggagcgcga gccgatacgg ttgacagctt cgggggcggc gggccggtga gcggccgttg
     8101 gcggcttggc gaataaagac aacgggcggg gccgtgaggc gacccgcccg ttgccgtgcc
     8161 gatggacgtc gctgcgcgcg ccctggggag gttgcggggc gcgcggagcc gcgagcggcg
     8221 gggttaccgc cgcgggttga cagaggtatc tgcgtcgggc gcgcaggcgc cgggaccgat
     8281 gtcgccgacg ttgaaggcgt gcagggcgcg gtaccactca tggatctgtt tgaaggcgcg
     8341 gcgatcgacc gaccggcggc tgtccaccgg gtgggtggcc aggtaggcgt cggcggcggc
     8401 gatcaccccg gcgaccgcgg agtcgtcggc gccggcggcg atgttgagtt tagcggccag
     8461 cagttcggtc agcaggggct tgaccggcgg gaaggtccgg tcgtgattcc ggcgctggag
     8521 ggattgggcc gctttgcagt tggagtcgat ttcgacgctg tgggcgccgc cgggcgtccc
     8581 cacccagagg gggagcaggg ccgaaatctc atcggactcc gaccccttgg cgcagccgct
     8641 gtggcggagc caccagggcc aggggcgggt gcacccttga tcgtccggat cgcccggttc
     8701 gatgatggtg gtgtccggcg gatcggtgat ggtggtgtcg ttgggctcgt cgatgccggt
     8761 cgagtcgcca tcgggcggcg gcggattata gaccggcaga tcgaacagca ggaggctctg
     8821 gtttttcagg ccgccgtcgg ccgcccagcc ggtgaagagc agttgggcca ggccgagcgg
     8881 gttgttcggg acggactcat cgatagcagc gtcgcggagg accggaaact gaaccgaggt
     8941 gtcgccggtg gggccgccgt aggtgacggt caggtagggt tgcatgaagg cgttttcgcg
     9001 gctgaagaac atcccgcgcg ggaagaggtt ggtggtctgt cccaggagca ggccgcagtt
     9061 cggggccgcg ccgccgaccc actggcgcac cagcgccgtc acctcggcgg tcttccaccc
     9121 cggcgccgcg gcggcgaaag aaccatgcac ggccgccgtg tcgtaggccc cggcgaaact
     9181 gttccaggtg acggttgatt ccgcccacgg ggcggtcacg cggtagacgt tcacagtctg
     9241 caggctcggg ccggagcagt agacggacag ggccgcggtt ttgaccgagt cggggacaat
     9301 gacgccggcc gcgagggcga agggttgatc ggcggtagat gccgacccga cggggctgcg
     9361 gtcggagcag ccgaccaggg ctgccaggag cccggtgaga gcaagggaag tgattttctt
     9421 tctcataccg catcctcctt cagaggcgtc ggacgaacgc atcggtaggc gctgacaatc
     9481 tctctgcaag ccggatgcgg ggcgggaagt tttttccggg ttagtgtaag tcattgattg
     9541 aaagtcgctt ggcggaccgg gccggtttcc gggagaggtg gctgcagggg tctttggtgg
     9601 tggcaggctg agcagggtga gcgatccttg cgcagacggc ggagggccgg cggccgtgcg
     9661 tgccggctgt tacgaccagg ccgtcatcct cggggatgac ggcctcggag aaagaagctt
     9721 tgggggggcg cggcctattt cacacgggcg gactgccccc ggcgttcggt gacgatgcgg
     9781 agggtgtcac gggcgatgac gagctcctcg ttggtcggca cgacgagcac gtggccgccg
     9841 gcgccggcgg aggagatgtc ggtttccgcg gcgtgggcgg cgctgttctt ttccgggtcg
     9901 acctggaggc cgaggaaggc gagtccctcg cagacttttg cgcgcacgac ggcggagttt
     9961 tcgccgacgc cgccggtgaa gatgagggcg tcgatcccgc ccatggcggc cgcgtatgcg
    10021 ccgacgtact tcttgatccg gtagcagtag atgtcgaggg cgagctgggc gttggcgttg
    10081 ccggcctggg aggccgactg gatctcgcgc atgtcggagg agacgccgga gatgccgatg
    10141 agcccggagt gtttgttcag cagggtgttg gcctcgtgga gggagagttc ctcgcgggcc
    10201 atgaggtgga ggatgatcgc cgggtccatg tcgccggagc gggtgcccat gagcagcccc
    10261 tccagggggg tgaagcccat cgaggtgtcg accgagacgc cgcggtcgac ggcggtgatg
    10321 gaggcgccgt tgccgaggtg ggcggtgacg actctcaatt cggcgagcgg gcggcggagc
    10381 atgacggcgg cgcggtcgct cacgaacttg tgggaggtgc cgtggaagcc gtagcggcgg
    10441 atgccgtagc gcttgtacag gacgtagggg atgccgtaga tgtaggcgta gccgggcatg
    10501 gtgtagtgga aggcggtgtc gaagacggcg acctggggga gtccggggag ggtgcgctcg
    10561 caggcggtga tgccgcggat gttgtggggg ttgtggaggg gggcgagttc gatgaggttg
    10621 cgcagttcct gcatgagggc gggggtgatg agcaccgagt cggtgaattt ctcgccgccg
    10681 tggacgacgc ggtggccgac ggcgccgatc tcggcgcggt ccttgatgac gccgtggttg
    10741 ggcgacagga gcatggtgac gatgtattcg acggcctgga tgtggtcgag gatctcgccg
    10801 gagactttca cctcggggcg gtcgaacggt ttgtgggcga ggagggatcc ggacatgccg
    10861 atccgcgaga ccattccttt ggccagggct ttttccgtct ccgagtcgat gagctggtat
    10921 ttcacggagg acgacccggc gttgagcacc agtatcttca tggcggacgt tccttatttt
    10981 gtcacggcag ccgcggcggc cgggttgggg acgcggttgc cctcgttgtc atagacgaag
    11041 aagccgcgtc cggtttttac gccgaggtgg ccgctgcgga ccatcttgcg caggaggggg
    11101 caggggttgt acttgtgctc ggcgagttcg tcgagcaggt tctccatcca cttcatgacg
    11161 acgtcgagtc ccatcacatc ggccagggcg agggggccca tgttgaagcc gaaaccgagt
    11221 ttcatggccc ggtcaatgtc gtcggccgaa gcgacgcctt ccatgagcac gtgcatggcc
    11281 tcgttcagca gggggacgat gatgcgggtg gtgacgtacc ccgggtactc gttgaccgtg
    11341 atgtactcct tgtcgaggag ctgggcgaag gcgacggctt tgcggaaggt gtcgtcatcg
    11401 gtgtggaagc ccttgacgat ctcgacgacg gggaccttgg tcaccggggt gaggaagtgc
    11461 atgccgatga ttttctgggg gcgttcggtg acggcggcga tttcggagac ggagatggtg
    11521 ccggtgttgg tgaccaagag ggcgtcggga cggacgacgg tgtcgagttt ttcgaagagg
    11581 tcgcgcttca tctggaggtc ttcggggatc gcctcgagga tgatttcggc gtcctcggtc
    11641 atggagaggt cggaggaggg gtagatgcgg gcgaggatgg ctttcttgtc ggactcggtg
    11701 aggccccatt tgccgatctc ccggttcatg gcttcggcga tggctttgac gccgcgttcg
    11761 gcgagccggg tggttttgtc gatcagggtg acttcggtgc cggcggcggc gacggcctcg
    11821 gcgaggccct ggcccatcac accggcgccg atgattgcta tcttttttcc ggccatgtca
    11881 ttagtcctat gtgtcaggtc ttcaagtcta tcggttttgt cgacgaagga gtcgacaaat
    11941 caccccgaat ataattgctc tttcctgcag ggcaaccgca aattatactg cgggaggtat
    12001 gatcggcccc gccgctcggc gaaagcggcc ccggcgtgta caagggagga gacaaaccgg
    12061 gtccggctcg gcggcgtcga gccgggcgca ccgggtgggc ggccgcgaag atccagacgg
    12121 aaggagcagc tatgaagcag gcgataccct ttatcttgtt gttactcttg gcgataggtc
    12181 tcattgccgc acagccggcc gaggcggcga tacagcgggt cgcggcccgc tacaatatgg
    12241 tggatgtcta cggggggtat gcgagcccgc acggagagta tggcgaggtc gggctggtaa
    12301 tgtttattga cgagtttgac cggccggtgg aggttgacgc ggatcaggtg ttcgacgccg
    12361 gctggtcttt gggcttcaat tacggtactc tgtacgccga ccgctttctg atgcagctcg
    12421 gatttcgcta tacgaaccac caggagcagg actggttcaa ggaccatccg gatgagttcc
    12481 acctggcgca gtacgatctt aacctcaacc tgcacgtgta cgcggtgccg ccgacgaagt
    12541 cgattgtggc gccgtacggc ggtgtggggg tgcaggcggg cttgctcaac attgagatcg
    12601 ccgggtatga cgacgagtcc gatctgacga ttctgatggc ggcggatttc ggcttggacc
    12661 tgaaggtggc cgagttttcg aaggggcgga gttttctcac gctcgcttcc atcaacagct
    12721 atgacctgtt cggcaccgag gaccggccga agtacctcac gatcggcggc gggctgcgct
    12781 actggttccg ctagccgcag acagtgacgg cggcgggcgg gccgctgtct ggcgagagac
    12841 gtgtcaaggc gcgacggcgc aaggggaact atgagaggac gtgcggcgag ggcggcagtg
    12901 gcgttcgggc tggccgcggt gatggcggcg ggggggtgcg gcagcgattc gcccaacggg
    12961 ccgcagccgc agggcggtca ggtcgtggcg ctcaacggag tgcgtttcca gggattcatg
    13021 gggagcgatt cgacgacgac ggcggcgcag ttcgcggtca ccgacgcggg cggaaaccgg
    13081 ctggccaatc accagataca ggtcgcgccg gtgcgggggg acggagcggc gccgcgaagc
    13141 attgcgaccg acgcggccgg cgtggccgtg ctgcagtatg acttcagcgg acgcatgggg
    13201 cacgcggcac tgcggctgta tgcggagggg accgacacgc tggaggttta tttccgggcc
    13261 gatgcgatta tcccgggggt ccacggacag gtggggtacg tgctgttcga cgatgactac
    13321 gccgaggtca agcggtggct cggccagccg gagtcggagg atgtgcttcc gaaccggagc
    13381 cccgtgtacg cgtcctggga gtccacccgc ggcctggtcg tgatgttgta cgattgggat
    13441 ctggatggga agctctatga ttcgtcgagc gtctacggag cgatcgccgt gacgggattc
    13501 gcggggacga cgccggacgg cgacgcggtc ggcatgggct cgacgatcgc cgaggtacgc
    13561 aagcggtttg ggccgccgac ggattggggt gtgtaccccg aggagccgaa tacgctccag
    13621 tactatttcg gcctgtggga cctgtacgtt tttgcggacg gctcgcagcc cgacaccccg
    13681 gcggtgcaga tgacgctcgg cgagtatatc ccggcgtttc cgacggagat tgttcccctc
    13741 aatggtctgc tctaccgcgt ggccaacctt cccggcggca tgacctatcc ggcgctgcag
    13801 tttgccgtgg aggattcctc gggcgcgcgg atcggcaacg tggacattca gctgtcgcat
    13861 gccgccggag cgggatcttt cctgtcgacc ggcggagggt ccatctcgta cgtgacgacg
    13921 gcgacgaccc cgcccggtct tgcggaattc cgatatctca tgacccccgg tctgagcgga
    13981 agcgccgtgg ggcggctcac gataccggtg atcgacacgc tcgacatcta tctgcggcgc
    14041 aatgtgctgg cgccggggtc cggagggcag gggcagtacg tgctgttcaa cgaaacctac
    14101 gcggaggcgg cggcgtggaa cgggacggcg gcgagcgtgg ccggggacgg ccggttcggg
    14161 ccgacctacg cgtacgcggc ctaccccgat tccggtctca cgttcgtatt ccgcgatccg
    14221 ggcggaacca accaggtgac ctcgtcgtcg caagtgtacg gggtgatcgc cgaggcgccc
    14281 tggccgggga ccatggcctc ggcctccgcg gcgcagcgcg gaatccggat cggatcgacc
    14341 tatgcggagg tgaccggggc gtacggggcg ccgagcagct cggccacggc gggggacacg
    14401 ctcgggctgg tctacgacgg ttacccggtg aggtttgaac tggtcccggc cgtggacggg
    14461 gcggtggtcc ggatcctcac gtgggagggg ccgtgagcgg gggcgcggcc gaggggcggc
    14521 gcttgacagc ggccggcggg cgtcatatat tcgcggccgc ggctggaaga agaagaaccc
    14581 aggtttccac gttatcatag agaggataga gcgacatgcg atttatgacg agcgtgctga
    14641 tgattgtggc ggcggcgttt gtcctggtga tcggctgttc gagcaacgat gcgcagtcgg
    14701 ccgacaacac agcgcaggcc tcgggcaaga cgacggcgca ggccccggcg gcgaagccgg
    14761 cggcggcgac aaccggcaac ccttattatg acagtctggc gcagatgacc catcccattc
    14821 gcgacgagaa gaatccgatt gtcacgatcc gcacggattt cggtgacatg gtgtgcgagc
    14881 tgtaccgcga tgtggctccg gcccacgccg attcgttcgt ggccctgacc aagaaggggt
    14941 tctacaacgg gctcaccttc caccgcatca tcgacggctt catgatccag gggggagatc
    15001 ccaaggggga cggtacgggc ggcccgggct actcgctgac ggcggagttc tcggagcttc
    15061 cgcacgcgga cgggacgctc tcgatggcgc gttcgaacga cccgaactcg gcgggcagcc
    15121 agttcttcgt ctgcctcggc cgggcggcct tccttgaccg gaagtacacg gtgttcgggc
    15181 aggtgct
//
LOCUS       DAOPOE010000079        15037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4696_length_15037_cov_5.465025, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000079 DAOPOE010000000
VERSION     DAOPOE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15037
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_15037_cov_5.465025"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKM94_10390"
     CDS             <1..856
                     /locus_tag="PKM94_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD67081.1"
                     /translation="LIDRIDHATSRVEGVRITDNKNPARPQIIVAEYGYLKMLDNNLT
                     MQFTLYNGEVHQLDTQKRENYRRLDFESQVINIAESGQSELKRTDSEYRTDREMGLEQ
                     MRESVSVAEAAIQPFMEKVGQAVDNRTTYLLSDTFRFAAADIKTDSAAFLAVQRDAEA
                     QYRSLQRTIDQIEGQRRVRNKYLIEIYKKYSIPVASLAFILIGVPLAVMTRRSGMGMA
                     IAISIILYVVYWAFLIGGEDLADRGLVSPFLAMWSANILIGAIGVYLLYKVISERPVF
                     AWFRRGSA"
     gene            869..1957
                     /locus_tag="PKM94_10395"
     CDS             869..1957
                     /locus_tag="PKM94_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD67082.1"
                     /translation="MVPVKKLDRYLLKSFLLALVVVTVAVGLTINVINIIEELRDFID
                     NDVPVASIAEYYLYFGGWVVKSFLPMFVLIASLFSVSMFARRNEILAMKASGISLYRI
                     ALPYLAAAAALSVGHFYYNEYVYPPANQRRLEIKEFTIEKRSRQATSQVGNIYRQIEP
                     GHIYTIEGFNVDRRAGLSVRVYRTADDRLRKIITAERLTYDNQTWVLHDGVERTFSDS
                     LGETYREFADLPYPDIREKPEDFAKKIGDPADMGLVELRGYIDLMKRTGGPYHREEVD
                     LGFKYAYPVASFIVVLISLPLASNPRRGGVAVSISAGALISLVYFVTFRVLQSAGYNQ
                     KIPLEVSVWGVNALFFLVGVVLMLRARK"
     gene            1957..2730
                     /locus_tag="PKM94_10400"
     CDS             1957..2730
                     /locus_tag="PKM94_10400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD67083.1"
                     /translation="MGRPDHEQNRRFWDEIADIHYRHPDYRVAEFLAGRSTLRPIERR
                     LLGEVGGRTLLHLFCQFGLDTLSWARLGADATGVDISGRSIALAGELRRQTGLRAEFV
                     QADVLELIGRLDRTFDIVFQSYGTHHWISDLDLWARTVAHYLGPGGVLCLVDIHPLAP
                     VCLEGARYFARGARRRRDPDYADKDYRPAEESVQWAHTLGEVVTALAGAGLAIDRLEE
                     FDGFCYPYREGWVERDGYWYPPEGPPPYPMLFALLARKP"
     gene            2749..3096
                     /locus_tag="PKM94_10405"
                     /pseudo
     CDS             2749..3096
                     /locus_tag="PKM94_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            3166..4623
                     /gene="aspA"
                     /locus_tag="PKM94_10410"
     CDS             3166..4623
                     /gene="aspA"
                     /locus_tag="PKM94_10410"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOD67084.1"
                     /translation="MRQASTRVVNAGAQYVSGRTRAEHDLLGPREVPHEFYYGIQTLR
                     ALENFNISGISLMHFPVLIESLAMVKMAATQANHDLGLLPDDVTDAINQACREIIDGR
                     WHTQFVVDMIQGGAGTSTNMNANEVIANRALEILGHEKGEYQYVHPNNHINLSQSTND
                     AYPTALHLALIFSNRKLAKVLRRLIDAFQAKADEFHHVIKMGRTQLQDAVPMTLGQSF
                     QAFAETLSEEVTRLDQNAALFLEVNLGGTAIGTGINAHPDYADRAVARLRDISGLDLV
                     IAPDLIEATSDTGAYVMYSSALKRLAVKLSKICNDLRLLSSGPRAGLNEINLPKMQPG
                     SSIMPGKVNPVIPEVVNQIAFKVIGNDLTVTMAAEAGQLELNVFEPVIAQSIFESVEM
                     LKNGMDTLTFRCVKGITANEEVCRRHVAQSIGVITALNPVLGYDTSTALAQEAEETNR
                     GVYDLALEKGLLSKEELDRLLAPESMIRPRRRPEA"
     gene            complement(4707..6050)
                     /gene="gdhA"
                     /locus_tag="PKM94_10415"
     CDS             complement(4707..6050)
                     /gene="gdhA"
                     /locus_tag="PKM94_10415"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOD67085.1"
                     /translation="MSTFIASFIGELRNTHPGQPEFHQAVYDTLLSAAPALERTPAYQ
                     GANILGRIVEPERVVIFRVPWVDDGGRVRVNRGYRVEFSSALGPYKGGLRFHPGVNLG
                     VMKFLAFEQTLKNSLTTLPLGGAKGGSDFDTKGRSEGEVMRFCQSFMNELFRHLGPRI
                     DIIAGDIGVERREIGFLFGQYKRLRNAFDGAVMGKAVGWGGTVLRPEATGYGCAYFAA
                     EMLATRDESFAGKRVAISGAGTVALHAAEKVVALGGRVVAMSDSHGFVVDEAGIEGEK
                     LAFLAAEREGRRGTIREYADRFSGVSYSQGPHIWHVPVDIAMPCATQNEMNTQDAAAL
                     IANGCRCVCEGATMPVTADASRVLQEAGILYGPGKAANAGAAVVSAFETAQNASGLVW
                     SAAAIDRRLRAMMAQIHRTCLDTAAAYGSPGNYVNGANLAAFVRLADAVLAQGVV"
     gene            complement(6996..7997)
                     /locus_tag="PKM94_10420"
     CDS             complement(6996..7997)
                     /locus_tag="PKM94_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOD67086.1"
                     /translation="MKAMVLRQPHEPLVLTDLPDPKPGPGRILLKVHACGVCRTDLHV
                     FDADLTEPVLPLIMGHQIVGSVLAVGEGVSGVEPGDRLGVPWLGGSCGHCDYCRSGRE
                     NLCPTARYTGYQINGGFAEKTVADARFCFPIPPGYPDLQAAPLLCAGLIGYRSLRMTG
                     SARRLGLYGFGAAAHIVIQVARHQGRRVYAFARRGDTASQDFARSLGADWAGPSDELP
                     PEPLDAAIIFAPVGALVPLALQAVAPGGVVVCAGIHMTDIPSFPYRWLWGERVVRSVA
                     NLTRRDGEEFLELAPRVPIRTEVHPYPLNRANEALSDLREGNFTGAAVVVVDEDASG"
     gene            8542..8676
                     /locus_tag="PKM94_10425"
     CDS             8542..8676
                     /locus_tag="PKM94_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67087.1"
                     /translation="MIYLVDPANANRPPCFAKCKVLCDGKGCTTYCGIKPLYGIDPTI
                     "
     gene            9568..10947
                     /locus_tag="PKM94_10430"
     CDS             9568..10947
                     /locus_tag="PKM94_10430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD67088.1"
                     /translation="MPTLSRYNHFQPWQDGYYIAYNALSGAVALMTEENYAVYRRLVG
                     KLNGAATPDLSEEEAGLLKQLEYGAFVRSDGVDERALLRFNHNIARYDRTTLGFVIAP
                     TMACNMACVYCFENKTARKMSPEVMAAIVSYVEKRARSISGLQIDWYGGEPLLALDVI
                     ESLTASFVEMGEKHQFEYSSSMISNGYLLTAETVDRLVALKIRHVQITLDGPARIHNV
                     KRPLKNKKESFYRILENLKYASTKLSVGIRVNVDKSFTIDMIEELLAELTAAGLRERA
                     GVYFGMVEPSSQVCANIAESCYETADFSAIEVDYYRLLLARGFRIDKLPSPVAVFCMA
                     QSTNAHLVDPDGNLYRCWNHAGDAPKAYGTITDEINYLHPNFQRLFSADPFDHALCVE
                     CELLPICMGGCPARRADRQLAGTQMCEAWKHNLQPMLEIIARSKQQEMARRQQQEAEA
                     QAAEKESTT"
     gene            10944..12044
                     /locus_tag="PKM94_10435"
     CDS             10944..12044
                     /locus_tag="PKM94_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD67089.1"
                     /translation="MSDFHFFPEQVVWEITFACNMRCIHCGTSAGVPRSDELTTDEAL
                     RLIDELTGLGARGITLSGGEPLLRKDWPVLGKRIVANGAGAYLITNGFAVDEQAVDLF
                     QEVGLRNVGVSFDGSKEVHNYIRQRKTSYDKALHAMRLMAERKMRFEAVSQISKVNLK
                     DMDSIHKDLVSIGCPEWRIQMTTVTGRMEKEMVMGLAEYEQLIDKILEFKKDTRMMVD
                     VGENIGYYGCKGTQLLDGMPYLGCYAGVRVAGICSNGDVKGCLSQQPEFVEGNIRERS
                     FTEIWNDPTKFLYNREFTKATATGACHDCRYLPLCRGGCTTTSVSASGERANNPFCIW
                     QVEQKRGVQPIENDLIVELLSRFQEPQPAEKK"
     gene            12585..14363
                     /locus_tag="PKM94_10440"
     CDS             12585..14363
                     /locus_tag="PKM94_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD67090.1"
                     /translation="MVRGARMIPDPRTRRRLWSWLRPYWRLEALMFVIMLVMTALALM
                     LPQAIRYMIDDLIPSLRADTDGSVDMAPAVWFGLALIGLYLSSFVFSIGRDYLAGKVG
                     ASIIADLRAALFDHLAQVPLRFYQTNNVGEIMSRLLADVQRVQSLLTTTLLMFLTNIL
                     MLLGILVTLFVFNWIWAALALIPVPIIVWLSHRYGISLHAINYRLQESIAQLSGRLQE
                     VFSTIRTVRAFAQERREKTRVVGVLSAMTGLYIRNSVMTSLAVNLVQLVASLVPVIVL
                     SWGAYLVAAGSFPLGALFMFYIYLGYLYSPIQELSQVNVEVQSAMASVYRIFEYFDLP
                     QAVAEDPQPVRLAEVRGRLAFEDVDFHYGGDNGFAIRALSLTIEPGEKVAIVGPSGAG
                     KTTLINLLLRFYDPEGGRILLDRVDLRRLALKELRSHMGLVDQDPVLFKASLRDNIAY
                     GRAEASMDRVIEAARVANIHDFIEKLPLGYETEVGERGVTLSGGERQRVCLARAILLN
                     PPIMLLDEATSALDTRSEELIQQALEKALQGKTSIIIAHRLATVRHADRIIVLDNGRI
                     VGEGTHEALLESSPLYGELARKQLLT"
     gene            complement(14397..>15037)
                     /locus_tag="PKM94_10445"
     CDS             complement(14397..>15037)
                     /locus_tag="PKM94_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD67091.1"
                     /translation="GQIGGLFDINLSGTADDLSRLRDEMMTDFHIAIILTYLLLVALF
                     QSFTYPFVVMLTVPLATFGGVLGLDIVQMVDPSQQLDILTMLGFVILVGTVVNNSILI
                     VHQGLNFMREGWDQRAAIKESVRVRVRPIFMTTGTTVLGMMPLIIMPGAGSELYRGIG
                     AVVVGGLAVSTLVTLVLTPLVFSYAIELVVKVRSLLGLSERAVGAVPSLEGE"
BASE COUNT         2713 a         4914 c         4850 g         2560 t
ORIGIN      
        1 cctcatcgac cggatcgacc acgccacgtc gcgggtcgag ggcgtccgga ttaccgacaa
       61 caagaatccc gcgcggccgc agattatcgt ggccgagtac ggttacctga agatgctgga
      121 caacaacctc accatgcagt tcacgctcta caacggggag gtccaccagc tcgacacgca
      181 gaagcgggag aactaccggc gcctcgactt tgaatcgcag gtgatcaaca tcgccgaatc
      241 gggacagtcg gagctcaagc gcaccgacag cgagtaccgg accgaccggg agatggggct
      301 cgaacagatg cgggagagcg tcagcgtggc ggaggcggcc atacagccgt tcatggaaaa
      361 ggtgggtcag gcggtggaca accggacgac ttatctcctg agcgatactt tccggttcgc
      421 cgcggccgac atcaagaccg actcggcggc ctttcttgcg gtccagcgcg atgccgaggc
      481 gcagtatcgg tcgctgcagc gcacgatcga ccagatcgag ggccagcgcc gggtgcgcaa
      541 caagtacttg atcgagatct acaagaagta ttcgatcccg gtggcctcgc tggccttcat
      601 cctcatcggc gtgccgctgg cggtcatgac gcggcggagc ggaatgggca tggcgatcgc
      661 catctccatc atcctctatg tggtctactg ggcctttctc atcggcggcg aggatctcgc
      721 cgaccgcggc ctggtgagtc cgtttctggc catgtggtcg gccaacattc tcatcggcgc
      781 gatcggcgtc tacctcctgt acaaggtcat cagcgagcgg cccgtattcg cgtggttccg
      841 ccggggaagc gcctgaaggt gtcggactat ggtgcccgtt aagaagctcg atcgctacct
      901 gctcaagtcg tttctcctcg ccctggtggt cgtcaccgtg gccgtcggac tgaccatcaa
      961 cgtcatcaac atcatcgaag aactccgcga tttcatcgac aacgacgtgc cggtggcgtc
     1021 gatcgccgag tactacctgt atttcggcgg gtgggtggtg aaatcgtttt tgccgatgtt
     1081 cgtgctgatc gccagcctct tttcggtgtc catgttcgcc cgccgcaacg agattctggc
     1141 gatgaaggcc tcgggcatct cgctctatcg catcgcgctg ccgtacctgg cggcggctgc
     1201 cgccctgtcg gtcggccact tctactacaa cgagtacgtc tacccgccgg ccaaccagcg
     1261 gcgcctcgag atcaaggagt tcacgatcga gaagcggtcc cggcaggcca ccagccaggt
     1321 cggcaacatc taccggcaga tcgaacccgg ccacatctat acaatcgagg ggttcaacgt
     1381 cgaccgccgc gccggcctgt ccgtgcgcgt ctacaggacg gccgacgacc gtctccgcaa
     1441 aatcatcacg gccgagcgtc tcacctacga caatcagacg tgggtgctcc acgacggggt
     1501 tgagcggacg ttcagcgact ccctcggcga aacgtaccgg gaattcgccg acctgccgta
     1561 tccggacatc cgggagaaac cggaggactt tgccaagaag atcggcgacc cggcggacat
     1621 ggggctggtc gaactgcggg ggtacattga tctgatgaag cgcacgggcg gaccgtacca
     1681 ccgcgaggag gtggatctcg ggttcaagta tgcgtatccg gtggcctcgt tcattgtcgt
     1741 gctcatcagt ctgccgctgg cctcgaaccc gcggcgcggg ggggtggcgg tgtcgatctc
     1801 ggccggggcg ctcatctcgc tggtctattt cgtgaccttc cgcgtgctcc agtcggccgg
     1861 ctacaaccag aagattcctc tggaggtttc ggtctggggg gtcaacgccc tgtttttcct
     1921 cgtgggggtc gtcctgatgc tccgggcgag gaagtgatgg gccgtcccga ccacgaacag
     1981 aaccgccgct tctgggatga aatcgccgac attcactacc gccatcccga ctaccgcgtc
     2041 gcagagttcc tggccggccg cagcacgctg aggccgatcg aacgccgtct tctcggcgag
     2101 gtgggcggcc ggacgctcct ccacctgttc tgtcagttcg gccttgacac gctctcgtgg
     2161 gcgcgcctcg gtgccgacgc caccggggtc gacatttccg gccgctcgat cgcgctggcc
     2221 ggcgaactcc ggcggcagac cggtttgcgg gccgagttcg tccaggccga tgtcctcgaa
     2281 ctcatcggcc ggctcgaccg gacattcgat atcgtcttcc agtcctacgg cacgcaccac
     2341 tggatcagcg acctggacct ctgggcccgg accgtcgcgc actatctggg accgggcggg
     2401 gtgttgtgcc tggtcgacat tcacccgctc gcccccgtgt gcctggaggg cgcgcggtac
     2461 tttgcccgcg gggcgcggcg gcgccgcgac cccgactacg ccgacaagga ctaccggccg
     2521 gccgaagagt cggtgcagtg ggcgcacacg ctcggggagg tggtgacagc gctcgccggg
     2581 gcggggctgg cgatcgaccg gcttgaagaa ttcgatggat tttgctaccc ttaccgggag
     2641 gggtgggtcg agcgcgacgg ctactggtat ccgccggagg gcccgccccc gtacccgatg
     2701 ctcttcgcgc tcctcgcccg caaaccctaa gagaatgagg tccgacatat ggaaccgacc
     2761 gccatcaccg ccgccctgcg ccgcaccgag ctgtttcgcg agatcgacgt cgatgagctc
     2821 gaggcgctgg tggccatcac cgtcgcccgg ccgctcgcgg ccggggaagg actttttcgg
     2881 cagaacacgc cccggcagaa cctctggatc atcgccgagg gaacggtgga actgtacatg
     2941 acgaccgcct ggggcgggga gaagcggctg gcgcgcttcg gcccggggga tttcctcggc
     3001 gagggggcgc tcatggacga ttacccgcac tcgacatcgg cccgcgccgt gactcccggc
     3061 accgtcctcg tcgtcagccg ggcgaacttc gccgccctcg tgacccggca gccggagatt
     3121 gcggtgaaag tgctttcgcg ggtggcgcgg gtgatctccc gccgtatgcg gcaggcgagc
     3181 acgcgggtgg tcaatgccgg cgcccagtat gtctccggcc gcacccgggc ggaacatgat
     3241 ttgctcgggc cgcgcgaggt gccgcacgag ttctactacg gcatccagac cctgcgggcg
     3301 ctcgaaaatt tcaacatcag cggcatctcc ctcatgcact ttccggtgct gatcgagtcg
     3361 ctcgccatgg tgaaaatggc ggcgacccag gccaaccatg atctcggcct cctgccggac
     3421 gacgtcaccg acgccatcaa ccaggcctgc cgcgagatca tcgacggccg ctggcacacc
     3481 cagttcgtcg tcgacatgat ccagggcggc gccggcacct cgaccaacat gaacgccaac
     3541 gaggtgatcg ccaaccgcgc tttggagatc ctcggccacg aaaaggggga gtaccagtac
     3601 gtccacccga acaaccacat caacctgtcg cagtcgacca acgacgctta tcccaccgcg
     3661 ctccacctgg cgctgatctt cagcaaccgc aaactggcga aagtgctgcg ccggctcatc
     3721 gacgcgttcc aggcgaaagc ggacgagttc caccatgtca tcaagatggg ccgcacgcag
     3781 ctgcaggatg ccgtccccat gaccctcggc cagtcgttcc aggcgttcgc cgagactctg
     3841 tccgaggagg tcacccgcct ggaccagaac gcggccctgt ttctcgaggt caatctcggc
     3901 gggacggcga tcgggacggg gatcaacgcc caccccgact acgccgacag agcggtcgcc
     3961 cgcctgcgcg acatctccgg cctcgacctg gtcatcgccc cggacctgat cgaggctacc
     4021 tccgacaccg gcgcctatgt catgtactcc tcggccctga aacggctggc cgtgaagctc
     4081 tcgaagattt gcaacgacct tcgactgctg tccagcggtc cccgcgccgg cctcaatgaa
     4141 atcaacctgc ccaagatgca gccgggctcc tcgatcatgc ccggcaaggt caacccggtc
     4201 atccccgagg tcgtcaacca gatcgcgttc aaggtcatcg gcaacgacct gacggtgacg
     4261 atggcggccg aggcgggtca gctcgaactg aacgtgttcg aaccggtcat cgcccagtcg
     4321 attttcgaat cggtcgagat gctcaagaac ggcatggaca cgctgacttt ccgctgtgtc
     4381 aaagggatca cggccaacga ggaagtctgc cgccgccacg tcgcgcagtc gatcggggtc
     4441 atcaccgctc tcaacccggt gcttggctac gacacctcga cggcgctggc ccaggaagcc
     4501 gaggagacca accggggggt gtacgatctg gcgctcgaga aaggccttct ctcgaaggaa
     4561 gaactggacc gtttgctggc cccggagagc atgattcgcc cgcgtcgccg gcccgaggcc
     4621 tgagccgcga tcgaggggag acatatcgga ctgcgacaga aagccgtgcg gaaaccgggc
     4681 ccggaggggg aggagtgagg gggtgtctac accacgccct gcgcgagcac ggcgtcggcc
     4741 agccgcacga aggcggcgag attggcgccg ttgacgtagt tgccggggct cccgtacgcc
     4801 gccgccgtat ccaggcaggt acggtgaatc tgggccatca tggcgcgcag ccggcggtcg
     4861 atcgcggccg ccgaccacac gagcccggaa gcgttctgcg ccgtctcaaa agcggagacc
     4921 acggcggccc cggcgttggc cgctttgccc ggaccataga gaatccccgc ttcctgcaga
     4981 acccgcgagg cgtcggccgt caccggcatg gtcgcgccct cgcagacgca ccggcagcca
     5041 ttggcgatga gggccgcggc gtcctgagtg ttcatctcgt tctgcgtcgc gcagggcatg
     5101 gcgatatcca ccggcacgtg ccagatgtgc ggtccctggg agtaactcac cccgctgaag
     5161 cggtcggcgt actcgcggat cgtcccccgg cgcccctcgc gctcggcggc aaggaaagcg
     5221 agtttctctc cctcgatgcc ggcctcgtcg actacgaaac cgtgggagtc ggacatggcc
     5281 acgacgcggc cgccgagggc gacgaccttc tccgcggcgt ggagagccac cgtgcccgcc
     5341 cccgagatgg ccacccgctt gcccgcaaac gattcgtccc gggtggcgag catttcggcg
     5401 gcgaaatacg cgcagccgta gccggtggcc tccgggcgga ggacggtgcc gccccatccc
     5461 accgcctttc ccatcaccgc gccgtcgaac gcgttgcgca gccgcttgta ctggccgaag
     5521 aggaagccga tctcccggcg ttccacgccg atgtcgccgg cgatgatgtc gatccggggc
     5581 ccgaggtggc ggaaaagctc gttcatgaag ctctggcaga accgcatcac ctcgccctcg
     5641 gagcggcctt tcgtgtcaaa atccgatccc cccttggccc ctccgagcgg caacgtggtg
     5701 aggctgttct tgagagtctg ctcgaaggcg aggaatttca tgacgccgag gttgacaccc
     5761 gggtggaagc gcagcccgcc tttgtaggga cccagagcgc tggagaattc cacgcggtac
     5821 ccgcggttga cccgaacgcg cccgccgtcg tccacccacg gcacgcggaa aatgacgacg
     5881 cgctcgggtt ccacgatccg cccgaggatg ttggcgccct ggtacgccgg cgtgcgctcg
     5941 agcgccgggg cggccgacag cagggtgtcg tacaccgctt ggtgaaattc gggctggccg
     6001 gggtgagtgt tgcgcaattc gccgatgaac gacgcgatga aggtggacat cgatgtacct
     6061 cgccgggcgc catccggatc gccgccgcgc ggagcccggg cattcgggaa acgaatcacg
     6121 ggtatatagc agaaccggcg gtctttgtca aggccgcccg acaggccggc cggtcatcga
     6181 ctagtttgtc cttgacaccg cattcccatc tttcttcttt cttatggggc gccgcgccgc
     6241 cgtgggtgcc gggtggcacc gataagtgta cggcgacgct cgagcagcct caccgcggac
     6301 ggtatggcag cgccgacgga gattctgggg ccgcccgcgg gaacccacga cagtgaccgg
     6361 cagaggatga ggctcacacc cgcccgataa gcgcacggac gctctggggc gccgccgcga
     6421 ccggacggga tgccctgtaa ctccattatc gtatgggagg tgcgcatgct ctttctgatc
     6481 tgcccggaca gtgcgggcac accgaaagtc tgcacgtggg tgtgcagcac gttgtgccgg
     6541 gtcaagccct gctacggcgt cgaccccatt tagtcgcatc gccttgcgga cgggatacgc
     6601 gcggcgaagt gtcccctgcg acaaacccgc cgattgtcgc atgacggtcg ccgacggcgc
     6661 ggacatttat cccgccgacc ggtcggcaaa aatgggaacg tccacacctg ccggagatcc
     6721 ggaaccgcca gccggcggct gcggcgccgg ccatccggac aggagttacc gaaacgtcct
     6781 gatatgggag gtgcctatgg tatacctgat cagcccggag tgcgtgtgca aggccaaggt
     6841 gtgcacctgg ctctgcgtca caaactgcgg gatcaagccg tgctacggcg tcgatcctga
     6901 cccatggtga ttcctgtgtg tcgtccgggg aggttcgcgt atggacctcc ccgccgatac
     6961 tccgcctgtc cattccccgc cagttccgag agcgctcacc ccgaggcgtc ttcatccacg
     7021 acgacgaccg ccgcgccggt gaagttcccc tcgcgcaggt cgctgagggc ctcgttggcc
     7081 cggttgagcg ggtacggatg cacctcggtc cggatcggca cgcgcggggc cagctccaga
     7141 aactcctcgc cgtcgcggcg ggtgagattg gcaaccgagc gcacgacccg ctccccccac
     7201 agccagcggt aggggaacga ggggatgtcg gtcatgtgga taccggcgca cacgactacg
     7261 ccgccggggg cgactgcctg cagggccagc ggcacaagcg cgccgacggg agcgaagata
     7321 atcgccgcgt caaggggttc cggagggagt tcgtccgacg gccccgccca atcggccccg
     7381 agcgagcggg caaaatcctg gcttgcggtg tccccgcggc gagcgaacgc atacacgcgc
     7441 cggccctgat gccgggcgac ctggataacg atgtgcgcgg ccgccccgaa cccgtacaga
     7501 ccgagccgcc gggcagagcc ggtcatgcgc aaagagcggt acccgatcag gccggcgcac
     7561 agcaggggag ccgcctggag gtcaggatag ccggggggga tgggaaaaca gaagcgggca
     7621 tcggcaacgg tcttttccgc gaagccgccg ttgatttggt acccggtgta acgggctgtg
     7681 ggacagagat tttcccgacc gctgcggcag tagtcgcaat gcccgcaact cccgcccagc
     7741 caggggacgc cgaggcggtc gccgggctca acgcccgaaa cgccctcgcc cacggcaagt
     7801 accgatccca caatctgatg ccccattatc agcggaagga ccggctcggt caaatcggcg
     7861 tcgaatacgt gcaggtcggt ccgacagacg ccgcacgcat gcactttcag caggattctc
     7921 cccggacccg gtttcgggtc gggaagatcg gtcaaaacaa ggggctcgtg cggttgtctc
     7981 aaaaccatcg ccttcatgag ctatcaactc cattgtcgtg ccgtgatctg tcagcattcc
     8041 ggcgcgtgat ttcccgccga aaggtaaggt attacccgtt cgcattcaag ctgtttcgcg
     8101 cagagcggag atgcgcatct tattcgctac aaacattatc ctatttatgt gccatactcg
     8161 accggtttgt ccttgacaga attgtcctcg ttgcataatt ttgacagaaa gaggctcgct
     8221 ctggtggctc cgcctccatc gcggaggaga attcggacaa accgttttct tggacctggc
     8281 ggtgtggcat cgccgcatga gtgtctgaga ttatcgcctg catactgcat tgattagtaa
     8341 actcggatat tccatgcctg ccgaatgcag tcgaatagtc gaaattcatc ggtgttcgga
     8401 caggtcgaaa tgtattcacc gcttgctgac gggaggtgac gcgctgcccc tggtcgcttg
     8461 tgtgcggact gatatactgg cgagttgatc cggggcggag cggacgcccg aacggcaggc
     8521 caacaacagg aggtctttgg catgatctac ttggtcgatc ccgccaatgc gaatcgcccg
     8581 ccgtgctttg cgaagtgcaa ggtgctgtgc gatggcaaag gctgcacgac ttactgcggc
     8641 atcaagccgc tgtacggtat cgatccgacc atctaaaggt cggagaagtg actcaggccg
     8701 ggagggctcg cagagccctt ccggcattgc tcgcagttga gtcggacgcc gtcgtgaccg
     8761 gtcaaggagg gggtggcacg gcccacacga acaaaccgca gctcccgcga ataccgcagc
     8821 accggaaagt gtgcaccagg taagtgccat cgggcgtggc gggaggttct caggcgcccc
     8881 tgatcggctc tttgccggcc gaggacgcgg ccaggcagtc cggggcgagc agcaagatga
     8941 ggcgttactc agggttcaca gcgcccgttc gaggggactg ggagcccggc cataaccttt
     9001 cacgcgtatg gaggtgtcgg catggtctat ctcatcgacc cgcaagggct ggggaagaaa
     9061 ccgccctgca agacactgtg ctatccggtg ttttgcgcga tcaagccgct ctacggaatc
     9121 gagccgtaat gccgaattcg catccgggag agggagctgc tttcccggat gcggtcgaga
     9181 agaaaggccg gggccgtccc cggtgtcttg agaagacagg aaggcgcagc ttgagttaat
     9241 ggctgtgcga ggtctcgtgg taccgcaacc gaccgaaggg aggtgaactc atggtctacc
     9301 tgcttgaccc ccaggaaatg ggtaatcgcc cgccgtgcac gcggtgcaga attaagccgc
     9361 tttacggtat tgacccggtc ccgatttgat cgcacgaccg aattgcccgc agcccgggtt
     9421 tccgggctgc ggggcagagg gcgtcgcgga acagacgccg acatgaggtg cggccgcggg
     9481 ctgaacagct gcggccggaa caaccgcctg tggggcagaa ccgggcactg ccccatcgcg
     9541 cggagacgca aatgatggaa acggaagatg ccgacactct cgcgctataa tcacttccaa
     9601 ccgtggcagg atggctacta catcgcctac aacgccctgt ccggagctgt ggcgctgatg
     9661 accgaggaga actacgccgt ctaccgccgg ctcgtcggga agctcaacgg cgcggcgacc
     9721 cccgacctga gcgaggagga ggccggtctg ctcaagcagc tggagtacgg cgcgtttgtc
     9781 cgctccgacg gagtcgacga acgggcgctc ctgcggttca atcacaacat tgcccgctac
     9841 gaccggacca cgctcgggtt cgtcatcgcc ccgaccatgg cctgcaacat ggcgtgcgtg
     9901 tactgcttcg agaacaagac cgcccggaaa atgtcccccg aggtgatggc ggcgatcgtc
     9961 agctacgtcg aaaagcgggc ccgctcgatc agcggtctcc agatcgactg gtacggcggc
    10021 gagccgctgc tggctctcga tgtgattgag agcctcaccg cgtcgtttgt ggaaatgggc
    10081 gagaagcacc aattcgagta cagctcgtcc atgatcagca acggctacct gttgacggcg
    10141 gagacggtcg accggctcgt ggccttgaag atccgccacg tgcagatcac gcttgacggc
    10201 cctgcccgga ttcacaacgt caagcgcccg ctgaagaaca agaaggaaag tttctaccgg
    10261 attctcgaga acctgaaata cgcctccacc aaactgagcg tcggcatccg ggtgaatgtc
    10321 gacaagtcct tcacgatcga catgatcgaa gagctcctcg cggagctgac ggcggccggt
    10381 ctccgggagc gggccggcgt ctacttcggc atggtcgagc cgtcctcgca ggtctgcgcg
    10441 aatatcgccg aatcatgcta cgagacggcc gatttctcgg cgatcgaggt ggactactac
    10501 cggttgctgc tcgcccgggg gttccggatc gacaagctgc cgtcgccggt ggcggtgttc
    10561 tgcatggccc agtcgacgaa cgcgcacctg gtcgatcccg acgggaatct gtaccggtgc
    10621 tggaaccacg ccggcgatgc ccccaaggcg tacggcacga ttaccgacga gatcaattat
    10681 ctgcatccca atttccagcg tctgttttcc gcagatccgt ttgaccacgc cctgtgtgtg
    10741 gaatgcgaac tgctcccgat ctgcatgggc gggtgtccgg cccggcgggc cgaccgccag
    10801 ctcgcgggca cgcagatgtg cgaggcctgg aaacacaacc tgcagccgat gctcgagatt
    10861 atcgcccgct ccaagcagca ggagatggcg cgccggcagc agcaagaagc ggaagcgcaa
    10921 gcggccgaga aggagtcgac aacatgagcg attttcattt tttccccgaa caggtggtct
    10981 gggagatcac gttcgcctgc aacatgcgct gcatccactg cggcacctcg gcgggtgtcc
    11041 cgcgcagcga cgagctgacg accgatgagg ccctgcggct gatcgacgaa ctgaccggcc
    11101 tcggggcgcg cggcatcacc ctctccggcg gcgaaccgct tctgcgcaaa gactggccgg
    11161 tgctcggcaa gcgtatcgtc gccaacggcg cgggcgccta cctcatcacc aacggtttcg
    11221 ccgtcgacga acaggccgtc gatctgttcc aagaggtcgg cctgcgcaac gtcggcgtgt
    11281 cgttcgacgg ctccaaagag gtgcacaact acatccgcca gcgcaaaacc agctacgaca
    11341 aagcgctcca cgccatgcgc ctcatggccg aaagaaagat gcgcttcgag gccgtcagcc
    11401 agatctccaa ggtcaatttg aaggacatgg acagcatcca caaggacctc gtctccatcg
    11461 gatgccccga gtggcgtatt cagatgacca ccgtcaccgg ccgcatggag aaagagatgg
    11521 tcatgggcct ggccgagtac gagcagttga tcgacaagat tctcgagttc aagaaagaca
    11581 cccgcatgat ggtcgacgtc ggcgagaaca tcggctacta cggctgcaag gggacgcagc
    11641 tcctcgacgg catgccctac ctgggctgct acgcgggcgt ccgggtggcc ggcatctgct
    11701 ccaacggcga cgtcaagggc tgcctgtcgc agcagccgga gtttgtcgaa ggcaacatcc
    11761 gtgagcgctc cttcaccgag atctggaacg acccgaccaa gtttctctac aaccgggagt
    11821 tcacgaaggc gaccgcgacc ggcgcctgtc acgactgccg ctacctaccg ctgtgccgcg
    11881 gcggctgtac gaccacctcg gtgtcggcct ccggcgagcg ggccaacaac ccgttctgca
    11941 tctggcaggt cgagcagaaa cgcggcgtcc agccgatcga gaacgacctg atcgtggagt
    12001 tgctctcccg gttccaggag ccgcagccgg ctgagaagaa gtaagcgcgt tgggccgcac
    12061 ttctgcgccg cgcaaaccgc cggtccgccc gtcccgctgc agccgcccga cggggatgga
    12121 gcgggacgac cggaggacag gtctgttcag gccggacctt cggcgtctgc actccccgca
    12181 cgatctcccg cgcccgcccc ttggtcctgc acgattgtcc ctcggcccgc cggaaggacg
    12241 gagaggtgaa gccgccgcag gccggccgcc gggaagctgt aattccgggt tgttgcgggg
    12301 gcgcgcccgg agtatcttga cggcgacgag gtaaccttgg attcggaggc gacggtggag
    12361 atgcagttcg gtgtcggcag cggcgctcaa aaggccgtgc ggtcctgtcc cgcggccccc
    12421 cgggcgccgg gggaggggtc ggccgtcctg ggccggacac cgcaggaccg cggcttcggc
    12481 ctcgggtgcg tcgccggcgg cgggtgtgac cggccggcgg ctgcgcgaaa agcccgcccg
    12541 gtgcgccggc ccgccgcggg ccgggaatcc cgcggcgcac gggcgtggtc aggggtgccc
    12601 ggatgatccc cgatccccgc acccgccggc gtctctggtc ctggcttcgc ccgtactggc
    12661 ggctcgaggc cctcatgttc gtcatcatgc tggtgatgac ggcgctcgcg ctgatgctgc
    12721 cgcaggcgat ccgatacatg atcgacgacc tgataccgtc gctccgggcg gatacggacg
    12781 ggtcggtgga catggccccg gcggtctggt tcggcctcgc gctcatcggg ctgtacctca
    12841 gcagcttcgt cttctccatc gggcgcgact acctggccgg caaagtgggg gcctcgatca
    12901 tcgccgacct gcgcgccgcg ctcttcgacc acctcgccca ggtgccgctc cgcttctacc
    12961 agacgaacaa cgtcggggag atcatgtcgc ggcttttggc cgacgtccag cgggtgcaga
    13021 gcctgctgac gacgacgctg ctgatgtttc tgaccaacat tctcatgctc ctcggcatcc
    13081 tcgtcaccct cttcgtcttc aactggatct gggcggcgct ggcgctcatc ccggtgccga
    13141 tcatcgtctg gctgtcccac cgctacggga tttcgctcca cgccatcaac taccggctgc
    13201 aggagtcgat cgcccagctg tccggccgcc ttcaggaggt gttttcgacg atccgcacgg
    13261 tccgggcctt cgcccaggaa cggcgcgaga agacccgggt ggtgggcgtg ctgtcggcca
    13321 tgaccggcct ctacatcagg aacagcgtca tgacgtcgct ggcggtgaac ctcgtccagc
    13381 tcgtggcctc gctcgtcccg gtgatcgtgc tctcctgggg ggcatacctg gtggccgccg
    13441 gatccttccc gctgggggcg ctcttcatgt tctacatcta cctcggctac ctctactcgc
    13501 ccatccagga gctgtcccag gtgaatgtcg aggtgcagtc ggcgatggct tccgtgtacc
    13561 ggattttcga gtacttcgat ctcccgcagg cggtggccga ggatccccag ccggtccgtc
    13621 tcgccgaggt gcgcggccgg ctggcgttcg aggacgtcga tttccactac ggcggcgaca
    13681 acggtttcgc catccgcgcg ctctcgctta cgatcgagcc gggcgagaag gtggcgatcg
    13741 tcggaccctc gggggcgggg aagaccaccc tcatcaatct gctcctgcgc ttttacgatc
    13801 ccgagggcgg gcgcatcctg ctcgacaggg tcgacctgcg ccgcctcgcg ctcaaagagc
    13861 tccgctccca catgggcctg gtcgaccagg acccggtgct gttcaaagcg agcctgcgcg
    13921 acaacatcgc gtacgggcgg gcggaggcgt cgatggaccg ggtcatcgag gccgcccggg
    13981 tggccaacat tcacgacttc atcgaaaaac tcccgctcgg gtacgagacg gaagtcgggg
    14041 agcggggggt gacactctcg gggggcgagc gccagcgcgt ctgcctcgcc cgcgcgatcc
    14101 tcctcaaccc gccgatcatg ctcctcgacg aggccacctc ggcgctcgac acgcgcagcg
    14161 aagagttgat tcagcaggcg ctcgagaaag cgctgcaggg gaaaacctcg atcatcatcg
    14221 cccaccgtct cgcgacggtc cgccacgccg accgcatcat cgtgctcgac aacggccgga
    14281 ttgtcggcga ggggacgcac gaggcgctgc tggagagttc gccgctctac ggcgagctgg
    14341 cccgcaaaca actgctcacc tgaccgagcg cgcagccggg cgccgcgccc accgcctcac
    14401 tcgccctcaa ggctcggaac cgcgccgacc gcccgctccg acaacccgag caggctccgc
    14461 accttcacca cgagctcgat ggcgtacgag aatacgagcg gcgtcagcac gagcgtcacc
    14521 agggtggaga cggcgaggcc gccgacgacc acggcgccga tcccccggta cagttccgat
    14581 ccggcgccgg gcatgatgat gagcggcatc atgccgagca cggtcgtgcc ggtggtcatg
    14641 aagatcggcc gcacgcgcac gcgcaccgac tccttgatcg cggcccgctg atcccaccct
    14701 tcgcgcatga agttgaggcc ctggtgcacg atgaggatcg agttgttcac gaccgtgccc
    14761 acgagaatca cgaagccgag catggtcagg atgtcaagct gctgggaggg atcgaccatc
    14821 tgcacgatgt cgaggcccag cacgccgccg aaggtcgcca ggggcaccgt cagcatgact
    14881 acgaacgggt acgtgaacga ctggaagagg gccaccaaga ggaggtaggt gaggatgatg
    14941 gcgatgtgga agtcggtcat catttcgtcg cgcagccggc tcagatcatc ggccgtcccg
    15001 gagagattga tgtcgaacag gccgccgatc tgcccct
//
LOCUS       DAOPOE010000080        14875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4749_length_14875_cov_6.035830, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000080 DAOPOE010000000
VERSION     DAOPOE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_14875_cov_6.035830"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..296)
                     /locus_tag="PKM94_10450"
     CDS             complement(33..296)
                     /locus_tag="PKM94_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67092.1"
                     /translation="MVALVAASFALFVLGESFFEGCDEHCADENHEICTCAHCANSPR
                     LADESFPVAPADQGTHFPLCSRGTAVPEQLWIHAIDHPPRISC"
     gene            566..1309
                     /locus_tag="PKM94_10455"
     CDS             566..1309
                     /locus_tag="PKM94_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD67093.1"
                     /translation="MAREKKWWETFFEDFRPAFRREPPGETLKQARAIFRKLEMKKGM
                     RFLDCPCGFGRIGLPLARMGLKVTGVDLTRSYLDEFAASARRSGVRVELVHEDMRRIT
                     FDAQFHAAANLYTSFGYFPSDSQNVLVLRRIFRALRPGGRFLMSIINRDWILVNFTPS
                     DWSEVGGVRILQTRRFDYARSVMHDTWHFLKDGTEKVHRTTLRLYSFHELRALFERVG
                     FIDIEGCGSLDGRPTGRTNRDIFVLARKP"
     gene            complement(1337..3958)
                     /locus_tag="PKM94_10460"
     CDS             complement(1337..3958)
                     /locus_tag="PKM94_10460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD67094.1"
                     /translation="MFRYSRLWVFPAVLFSAVLFLYCGNDDTVTEPEPPRPVIVTGAI
                     DLPPESPLAPEDLTVLCGGGQGAVDSSGRFTAPVWEHSAAALLAVDTDHNPVLMGIVT
                     DPTAKGPVLLDARSTALALVFLNPFVCMADAAEADSILDALEAQVAFADFAALIGRKL
                     EADGLALTVDDPEIDSLLTEVVAEYVNSFVAEGVRSASLAPTPAAPTSPSEAPPTIQP
                     DYERSGHRLTWLGGTSFEISNSFGRWAYCCTPTDSFYVFPNGDFLDWVRKGQPWPPSR
                     RRLTLPVSVAETTVVNIYGYGCLGVADNRWEDLTPAEQLCAHYGGFVTVTLELCSHVL
                     GTVCNTARVAGKDAIAERINESILKPIFTDGRVYQRVAAYMEANDPWGCSWFLTKEVI
                     KKIVTSPGYREAFCIITGITLTDGMISTLASWLAIPAKVTLTFNSISSAFKTALALSS
                     ARYKTTFKVWKENAEFGNIEGMVADKTHGTGIAGATVTIGGDANNPMNPPHELATDAD
                     GHYRFENIGVGERTVTAAKAGYRTASVGVTVVKNSTVTADLELERTGAAVAGYVRNDI
                     LIHHSQPSTLFRDDVNIHCRQTGGDQDTYDTWALEGTLSFSLTQGTWWVVARHDDYDP
                     DSVLVTVPADGSVSLPRDLILKPHPTMTGRIYINTDNTGGYEIDFIPLFGQIGMSAPA
                     PYEYECPLGGSPADVMSAAAVRGTSESDYDAVEFAIRLEAVPEAGAYRIGGIDAYGCS
                     GLSAAAAAAFVTTRARCTIPESASYPMSYTFLDDPESRGCNCSIVQPGDIYLTEWSTE
                     LGEVVAGGFNIDLAGWNTCECAGSDTDSDGIIDTWEVDCSQARIQLDFRLPVGSDYLV
                     TFRPLGARQIALPPARD"
     gene            4176..4646
                     /locus_tag="PKM94_10465"
     CDS             4176..4646
                     /locus_tag="PKM94_10465"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD67095.1"
                     /translation="MEYADHRPPAEHRIRRLLADQFYGVLCTRGDGQPYGSMVAFACS
                     DDLRYLVFATPRGTTKYRYLCAGPRVAVLVNDLNRHPEDFMKVEAVTATGQASELPPA
                     ESPPWRRLLLQRHPYLERFRSSPATALFAVRIARYHFVSSFDDLMAWEPADAGG"
     gene            complement(4728..6680)
                     /locus_tag="PKM94_10470"
     CDS             complement(4728..6680)
                     /locus_tag="PKM94_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HOD67096.1"
                     /translation="MSMDQQPKRSSELGGADASRDAYENYELPIEGFKGTAEEIERQW
                     YERCYVGRGDAIKQLTWRAVIMGSVLGAVLSLTNIYIGLKAGWGFGVAITACILSYAI
                     WTTFYKLKLARTQMTILENNCMQSTASAAGYSTGGTLVSAFAAYILIHNETLSFSLMI
                     LWVFFIAVLGVTMAVPMKRQMINVEQLRFPSGIAAAETLRALHATGQKGMRAARALGI
                     AGLLAIVSAFLTDGLRLISARLESLQISALVGRFSSATLGETWIGRTVTFTWDPIFIA
                     AGMFVGMRVAASMLIGSITCWMIFVPWLQGNVTEAAGLTGFRDLVQWSLWAGTACMVT
                     SGILSVLFEWRAALRAFRGLGGMLAKRSGQALDAVAAIETPGSWFLIGQAVALVALAL
                     LAHKTFGMPYWQSFIAVLLSFALALVACRVTGETDTTPVGAMGKITQLTFGALSPGNM
                     DVNLMSANITAGAATSSADLLTDLKSGYLLGAHPRRQFLAQFAGIFIGTLVTVAAFRM
                     LVPNAGVLGTDQFPAPAAQTWRGVAEAMAKGLSTMHPVKIWCIVIGGLVGIILPILSR
                     VFPKHQKWIPSAAGVGLAWVFQWFYGLLFFLGAVIGWAWQKKNAKNCEEYLFPVASGV
                     VAGGALMGVVLVFWENGAEIIKQIFS"
     gene            complement(6917..8431)
                     /locus_tag="PKM94_10475"
     CDS             complement(6917..8431)
                     /locus_tag="PKM94_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOD67097.1"
                     /translation="MTFPIHVIPRPAEILPTGGGVRLRAPIPVHFAPNDARISRVIAV
                     GSDAWPGGEALWRPVEADQMKADPPGIHLLMEQGPEPVAESYRLLVGERGVRVNAASP
                     SGLFYGLQTLRQLLWPAETGEREIVLPGMEIRDAPRFGWRGMHLDVSRHFFPPAFIKR
                     YIDLLAFHKLNIFHWHLTDDQGWRIEIGRYPRLTEIGAWRTEPDGRRYGGFYTQAEIR
                     EIVRYAAARQVTVVPEIELPGHATAALAAYPEYSCTGGPFQVSNQWGVFDDVYCAGND
                     AAFTFLEDVLSEVADLFPSPYLHIGGDECPKARWQSHDLCRRRKDALGLADEDALQAY
                     FVGRVARFLRAQGKRVVGWDEILDGGAPEGAVIMAWQNVAKGSAAARAGHDVVMCPMS
                     NCYFDHYQGTSSEPQAIGGFTPLEAVHAFEPTPPDLPPDLLRRVLGAQGNVWTEYMAT
                     ENQVEYMAFPRLCALAEVLWTPPAARTFPDFLMRLRSHLRRLDTWGVNYRPLDD"
     gene            complement(8428..9480)
                     /locus_tag="PKM94_10480"
     CDS             complement(8428..9480)
                     /locus_tag="PKM94_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOD67098.1"
                     /translation="MTQRNRRPTASKTELFTVGVLAALALLNPGAGAPRAFETADART
                     IVVFRDHTIHFAPDDSAKYESGGVTVRDKGRVIAARVELPPFAGPVRITGRLALRPIP
                     KDELEMYDRWDRAGNVRLEVAGGPDVELIKFVTAHGGPTEFEVDLSPLAPLLAGPRVI
                     KGFVDTWVTPGWRMDFSLTYAPDSQALNADWAAGLLYEEAFTRDDPGAAGRAAVIEVP
                     EGMERVLLHYYVSGHCTDGTDADEFVSKDNVISVDGAVVYRYPPWRDDCRRFRAINPY
                     CRRWTDGSWSCDYSRSGWCPGDRAAPLELDLTDHLTAGRHTLRFVIEDIRPKDERGNY
                     GYWRVSGQLLGWRQPR"
     gene            complement(9464..>9707)
                     /locus_tag="PKM94_10485"
     CDS             complement(9464..>9707)
                     /locus_tag="PKM94_10485"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOD67099.1"
                     /translation="VVLETVKKAEKDAGLILRVAETHGRHSTGRLEIDFPNFTLEETD
                     LMERAVGRRLEAAPAAVLRMAPFEIRTLRLTYDTAQ"
     assembly_gap    9708..9717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9718..12565)
                     /locus_tag="PKM94_10490"
     CDS             complement(<9718..12565)
                     /locus_tag="PKM94_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOD67100.1"
                     /translation="MNTKDTLIAIRRLEQFYRRAVGGILREPAALAATCARSAEPVPF
                     AQRHGLACEPVAVGDVWGRNWESGWFHLVGRVPETYRGRTVVAHIDLGGEGLVYRPDG
                     RMLQGITNGSVFDSEFGRDVVRLFDPCAGGEPVELWVEAAANGLFGMFCDLDPGPDAA
                     NRDGRYDARVNTLRLAVFDTELWHLTLDLRVLLGLVRSLPERSVRRARIVRAAVDGLN
                     AYATSGESARAFREILARELKKPAAASSLAVTAVGHAHIDTAWLWPVRETIRKCARTF
                     ATQLDLLARYPDYVFGASQPQHYAFVKEHYPELYAQVRAAVKAGRWEPQGGMWVEADC
                     NVTGGESLVRQILHGKNFFRDEFGVEVDNLWLPDVFGYSAALPQLLRKSGIDYFLTQK
                     LSWNQTNEFPHHTFRWQGIDGSEVLTHFPPENTYNSQLHPESLAAAEERFRENDYLDE
                     FLCLFGVGDGGGGPKEEHIEMGRRQADLEGAPRVRFGPAREFFRRLAAERDRLPTWTG
                     ELYLELHRGTLTTQAYAKWANRRLEHKLRALEMLAAKLPLDRYPQQELDAVWKTVLLN
                     QFHDIIPGSSIGRVYRVAHTEYREALAGCERLAAAVAAELYPADGNCLTLVNTLHYPY
                     EGAVTLPAGWAGCEIDGGGPTPPCQREPDGVAVHVSIPPYAALTLRRTAETTAPAAPP
                     GQSLVLENDLVRYELAESGALLRCFDKALGLELVVPEAPGNILTLYEDRPNDWDAWDI
                     DAFYRETPLATARAAAAEPLPAGDVRRGVRFRYRIGRSHIVQTVRLGRMSRRLDFETA
                     VDWREKHRMLRVAFPVTVRSDYAAFDIQYGYLRRATHRNTSWEQARFEVVGHRYADLS
                     DRDHGVALLNDCKYGYRVENGLLDLNLLRAPNYPDPDADQGEHRFTYSLLPHPGDLAA
                     SEVMAEAARLNQGLLAAKGVRATNTRLPVRLSGEG"
     gene            complement(12585..14528)
                     /locus_tag="PKM94_10495"
     CDS             complement(12585..14528)
                     /locus_tag="PKM94_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOD67101.1"
                     /translation="MNLSLLDWAIVIVSLGLMIASVEGGRRVMRSVADFLSAGRSAGR
                     YVLCVASGAAGIGAITVVGNLEMNLIAGFSLTWWGMTTALVVLLITVSGWVIYRFRET
                     RSLTLAQYFEARYSREFRIFTGFIAFLSGIVNFGIFPAVEARFFIYFCGLPESVSLLG
                     FEVPLFPLMMLFLLGVSLYFVLGGGQVSVIYADFFQGVLVYGVFIALVVYFFVSFDWG
                     HIEQALAAAPRDASLINPFKTSQVEDFNFWYFLVGIIGVIYGTMSWQGTQAYNSSARS
                     AHEAKMASVLSSLRNLPMSIMFLFVPIVAYTVLNHPDFASIAAAVSDTLSGIEGKAVQ
                     SQVKVPLVLTHLLPRGLIGAFAAMMLAASISTLDAYMHSWGSIIVQDVIMPLRKKPFA
                     PAQHLRVLRWAIVAVGAFAFFFSLIFTQAEYIFLFFAITGAIFAGGSGAVIIGGLYWK
                     RGTAAAAWAAMITGSGIAVAGIIIHQIIPDFFINGQWFWGISMLGASLVYIAVSLLNR
                     REPFNIDRLLHRGRYAIQGERTVVNATPARGWKMLGMGREFTRGDKIIYILNYVWTGA
                     WLLVFIVGTIYNLTHEVSDGAWLSFWRTYLAIHVAVSSAIIIWFVIGGFRDLRRMNRR
                     LKVADRDHRDDGFVSESGPAPRT"
     gene            complement(14544..>14875)
                     /locus_tag="PKM94_10500"
     CDS             complement(14544..>14875)
                     /locus_tag="PKM94_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67102.1"
                     /translation="PEMWGLVQFSAAPPGSPEAALVADPADSARWALRQVYYAERNYQ
                     AAHGRFTAAHSDLALETAAPRGFTWPPALRATEDRFEAEVVRRDGGGALRITEDGRTA
                     PTGQAGG"
BASE COUNT         2561 a         4993 c         4878 g         2433 t
ORIGIN      
        1 cgccgggacc gggcttggcc ccgcggacgc gctcagcagg agatccgggg aggatggtcg
       61 atcgcgtgga tccagagttg ctcgggaacg gcggttccgc gagagcacag ggggaagtgc
      121 gttccctggt ccgcgggcgc gaccggaaac gactcgtccg ccagccgggg ggaattggcg
      181 caatgggcgc aggtgcagat ttcgtggttc tcatcggcgc agtgctcgtc gcacccctca
      241 aaaaacgact cgccgagcac gaaaagcgca aaggatgccg ccaccaacgc caccaccggg
      301 atcagtatcc cggcacaaga gagatctggt cggcggcgtc tcatgatagc ctgtaaccgg
      361 gccggccccg gttggttctc ttgaagttag gctatagtat acgtaattgc tccgctttcg
      421 gcaagagaaa agcttgctac cggccggggg ttcggaaggt gggacttgta cttgactggc
      481 ccgacccttc tgccttgctt tccccgtcgg cattcacgca gagttctgaa tcggcgcctt
      541 gtccagcagt gaaaagggag acggtatggc gagagagaag aagtggtggg agacattttt
      601 cgaggacttt cgaccggctt ttcgacgcga accgcccgga gaaaccctca agcaggcgcg
      661 cgccatcttt cggaagcttg aaatgaagaa gggtatgaga tttctggact gcccctgcgg
      721 gttcgggaga atcggcctgc cgctggccag gatgggtctc aaagtcaccg gcgtcgatct
      781 cacgcggtcg tatcttgacg agtttgcagc gagcgcgcgc agatccggcg tgagagtcga
      841 gctggtgcac gaggacatgc gccggataac cttcgacgct caatttcacg cggccgcgaa
      901 tttgtacacg tcgttcgggt acttcccgtc ggattcgcag aatgtgctgg tcctccgccg
      961 gatctttcgg gcgcttcgcc ccggcggccg gtttctgatg tccataatca atcgggactg
     1021 gattctcgtg aacttcacac cgagcgactg gtcggaagtc gggggcgtga ggattctcca
     1081 gacgcgccgg ttcgactatg cgcggtcagt catgcacgat acctggcact tcctcaaaga
     1141 cgggaccgag aaggtacacc ggaccacgct gcggctctat tcgttccacg aactccgggc
     1201 cctgttcgag cgggtcgggt tcatagacat cgagggctgc ggttctctcg atggccgccc
     1261 gaccggacgc accaaccgcg acatattcgt cctggcgcgc aagccgtgag cgggcggtgc
     1321 ggggtcgccc ggcccactag tcgcgggcgg gcggcagcgc gatttgccgg gccccaagcg
     1381 gtcggaacgt gaccaagtag tccgatccga ccggcaggcg gaaatcgagc tggatccgcg
     1441 cctgcgagca gtccacctcc cacgtgtcga tgatgccgtc gctgtcggtg tcgctgccgg
     1501 cgcactcgca ggtgttccat ccggccaggt cgatattgaa cccgccggcg accacctcgc
     1561 ccaactccgt gctccactcg gtcaggtaga tgtcgcccgg ctgaacgatc gagcagttgc
     1621 agcccctcga ttcggggtca tcgaggaagg tgtacgacat cgggtacgag gccgattcgg
     1681 ggatcgtgca cctggcccgg gtggtgacga acgcggcggc ggccgccgcg ctcagcccgc
     1741 tgcagccgta ggcatcgatg ccgccgatgc ggtaggctcc cgcttcgggg acggcttcca
     1801 ggcggatcgc aaactcaacc gcgtcgtaat cgctctccga ggtgcccctc acggcggctg
     1861 ccgacataac gtcggcggga ctcccgccga gcggacactc gtactcatag ggcgccggtg
     1921 cgctcatccc gatttggccg aacagcggga taaaatcgat ctcgtagccg ccggtgttgt
     1981 cggtgttgat gtagatgcgg cccgtcatcg tcggatgcgg tttgaggatc agatcgcgcg
     2041 gcaacgacac ggaaccgtcg gcgggcaccg tgaccagcac cgaatccgga tcgtagtcgt
     2101 cgtggcgcgc caccacccac cacgtcccct gcgtgaggct gaaactcagc gtcccctcca
     2161 gcgcccaagt gtcgtaggtg tcctggtcgc cgccggtctg ccggcagtgg atgttcacgt
     2221 cgtcgcggaa cagggtcgag ggctggctgt ggtggatgag gatgtcgttg cgcacgtagc
     2281 cggcgacagc ggcgccggtg cgctcgagct caaggtcggc ggtgacggtt gaattcttga
     2341 ccaccgtcac tcccaccgag gccgtgcggt accccgcctt cgccgccgtg acggtgcgct
     2401 cgccgacccc gatgttctcg aatcgatagt gcccgtccgc gtcggtcgcc agttcgtgcg
     2461 gcgggttcat cgggttgttg gcgtccccgc cgattgtcac cgtcgccccg gcaatccccg
     2521 tcccgtgggt cttgtccgcc accatcccct cgatgttgcc gaactcggcg ttctccttcc
     2581 acactttgaa cgtcgtcttg tagcgggccg aactcagcgc gagggcggtt ttgaaagccg
     2641 acgatatcga gttgaacgtc agcgtcacct tggccgggat tgccagccac gacgcgagcg
     2701 tgctgatcat cccgtcggtg agcgtgatcc cggtgatgat gcagaacgcc tcccggtagc
     2761 ccggcgaggt gacgatcttc ttgatcactt ccttggtcag aaaccatgag cagccccagg
     2821 ggtcgttggc ctccatgtag gcggcgaccc gctggtaaac ccggccatcg gtgaaaatcg
     2881 gcttgagaat cgactcgttg atccgctcgg cgatggcgtc tttcccggcc acccgggcgg
     2941 tgttgcacac cgtcccgaga acgtgcgagc acaactccag cgtcaccgtc acaaacccgc
     3001 cgtagtgggc gcagagctgc tcggccggcg tcagatcctc ccagcggttg tcggcgaccc
     3061 cgaggcagcc gtagccgtag atgttgacga cagtggtctc cgccacgctc accggcagcg
     3121 tcagccgccg gcgcgagggg ggccagggct gccccttgcg cacccagtcg aggaagtcgc
     3181 cgttggggaa gacatagaaa gagtcggtcg gcgtgcagca ataggcccac cgcccgaagc
     3241 tgttcgatat ctcgaagctg gttcccccga gccaggtgag gcggtggccg ctgcgctcgt
     3301 aatccggctg aatagtcggc ggcgcctcgg aggggctggt cggcgcggcc ggagtcggcg
     3361 caagggacgc ggagcggacc ccctcggcga cgaacgaatt gacatactcc gccactactt
     3421 cggtcagcag gctgtcgatc tccggatcgt ccaccgtcag cgccagccca tcggcctcca
     3481 gcttgcggcc gatcagggcg gcgaaatcgg cgaaagccac ctgggcctcg agggcgtcga
     3541 ggatgctgtc ggcctcagcg gcgtctgcca tgcacacgaa cggattgaga aacacaagcg
     3601 ccagcgccgt cgaacgggcg tcgaggagaa cgggaccctt cgctgtcgga tcggtgacga
     3661 ttcccatcag cacggggttg tggtcggtat cgaccgccag gagcgcggcc gccgaatgct
     3721 cccacacggg cgcggtaaag cggccggagg agtcgacggc gccctgaccg ccgccgcaga
     3781 gcacggtcag atcttccggc gccagtggcg attcgggggg aaggtcgatc gccccggtga
     3841 cgatcaccgg ccggggcggc tcgggctcgg tgaccgtgtc gtcgttgccg caatagagaa
     3901 acagcaccgc gctgaagagc actgcgggga acacccacag gcgggagtag cgaaacatgg
     3961 cggcctcctc tgaggcctcc caggcacagc ccagtcccaa acgcatggcg gcctatctgg
     4021 cggcaatata acaacttccc gcactctgtc aaggaataat ggacgaatcg cgcgctgttg
     4081 agcggtcccc gccgacgcgc ctcagggccc gccggaaatc cccgccgcga gcggttgcgt
     4141 tttggggccg cacccctatt atttccgccg ggactatgga atacgccgac caccgcccgc
     4201 ccgcggagca ccgcatccgc cgccttctgg ccgaccagtt ctacggagtc ctctgcacgc
     4261 ggggagatgg ccagccgtac ggttcgatgg tggcattcgc ctgttccgat gatctgcgct
     4321 acctcgtgtt cgccaccccc aggggcacga ccaagtaccg ctacctgtgc gccggccccc
     4381 gggttgcggt gttggtcaac gacctgaatc gccatcccga ggacttcatg aaagtcgaag
     4441 ccgtgaccgc caccggccag gcgtccgagc tccccccggc cgagtcgccg ccgtggcgcc
     4501 ggctgcttct gcagcggcac ccctacctcg agcggttccg gtcatcgccg gccacggcgc
     4561 tgtttgccgt gcgcatcgcg cggtatcact tcgtgagttc tttcgatgat ctgatggcgt
     4621 gggagccagc ggacgctggg gggtagcacg ttctccccgg gcaccgccgg gtaaaaaaaa
     4681 gccccgcccg gacagcggct gccgggcggg gtcttgcctg gtgattatca gctaaaaatc
     4741 tgtttgatga tctcggcgcc gttctcccag aacaccagga cgacgcccat gagcgcaccg
     4801 ccggccacca cgccggaggc caccgggaac aaatactcct cgcagttctt cgcgttcttc
     4861 ttctgccagg cccagccgat caccgcgccg aggaagaaga gcaggccgta gaaccattgg
     4921 aagacccagg ccaggccgac gcccgcggcc gagggaatcc acttctggtg tttcgggaac
     4981 acgcgggaga ggatgggcag gatgatcccg acgaggccgc cgattacgat gcaccagatt
     5041 ttgaccgggt gcatcgtgct cagccctttc gccatggcct cggccacccc ccgccaggtc
     5101 tgggcggccg gcgcggggaa ctggtcggtg ccgagcacgc cggcgttggg gacgagcatt
     5161 cggaacgccg ccacggtcac gagcgtgccg atgaagattc cggcaaactg ggcgaggaac
     5221 tgtctgcgcg ggtgggcccc gaggaggtat ccgctcttca ggtcggtgag caggtcggcg
     5281 gacgaggtgg cggcgccggc ggtgatgttc gccgacatga ggttcacatc catgttgccc
     5341 ggcgagagcg cgccgaaggt caactgggtg atcttgccca tggccccgac cggggtggtg
     5401 tccgtctcac cggtcacgcg gcaggctacc agcgcgagag cgaaactcag caataccgcg
     5461 atgaagctct gccagtaggg catcccgaac gtcttgtgcg ccagcagggc gagcgccacc
     5521 agcgcgaccg cctgcccgat caggaaccac gagccggggg tctcgatcgc cgcgaccgca
     5581 tcgagcgcct gcccgctccg tttcgcgagc atccccccga gcccgcggaa cgcccgcagg
     5641 gccgcgcgcc attcgaacag gaccgacaaa atcccggaag tgaccatgca ggcggtgccg
     5701 gcccacagcg accactggac caggtcgcgg aagccggtca aaccggccgc ctcggtgacg
     5761 ttcccctgca gccacggcac gaaaatcatc cagcacgtga tcgacccgat gagcatgctg
     5821 gccgccaccc gcatccccac gaacatcccg gcggcgatga agatcggatc ccaggtgaag
     5881 gtcaccgtcc gaccgatcca cgtctcgccc agcgtcgccg agctgaaccg ccccacgagc
     5941 gccgagatct ggagcgattc caggcgggcg ctgatcagcc gaagtccgtc ggtgagaaag
     6001 gcgctgacga tcgccagcag cccggcgatg cccagggcgc gggccgcccg catccctttc
     6061 tggccggtgg cgtggagcgc ccgcagcgtc tcggccgccg caatcccgct cgggaaccgc
     6121 aactgctcga cattgatcat ctgccgtttc atcggcaccg ccatcgtaac ccccagaacg
     6181 gcgatgaaga acacccagag aatcatgagc gaaaacgaca gcgtctcgtt gtggatcagg
     6241 atgtacgcgg cgaaggccga caccagcgtc cccccggtcg agtagcccgc cgcgctggcc
     6301 gtcgactgca tgcagttgtt ctccagaatc gtcatctgcg tccgcgccag cttcagcttg
     6361 tagaaggtgg tccagatggc gtaggagaga atgcaggcgg taatcgccac cccgaacccc
     6421 cagccggctt tgaggccgat gtagatgttg gtcagggaga gcaccgcccc cagcaccgaa
     6481 cccataatca ccgcccgcca cgtcaactgc ttgatcgcgt cgccgcggcc gacataacat
     6541 cgctcgtacc actggcgctc aatctcttcc gccgtcccct tgaacccttc aatcgggagc
     6601 tcatagttct cgtaggcgtc gcgcgatgca tccgccccgc caagctccga ggaacgcttt
     6661 ggctgctggt ccatcgacat acccttgttc tccttgcgag ttagtcggcc tggaacgata
     6721 gccgtcaact tacaccatct ttcgagccaa ggcaacgcga ttctgccgcc cgcgtcgtct
     6781 ccggcgcggc gttccgctcg gaccgcaaac cgcccaggca gcggaggccg caggtcatcc
     6841 gaccggcaac gcgccggctc ggcggcagcc gcggcgagca gtctcaacct aacgcagacg
     6901 gtattggttt tgtatgctaa tcgtccagcg ggcggtagtt gacgccccag gtgtccaacc
     6961 ggcgaaggtg actgcggagc cgcatgagga aatccggaaa cgtgcgggcg gccggcggcg
     7021 tccagagcac ctccgccagc gcgcacaggc gggggaaggc catgtactcg acctggttct
     7081 cggtcgccat gtactcggtc cagacatttc cctgggcgcc aagcacccgg cggagcaggt
     7141 cgggagggag atcgggcggc gtcggttcga acgcgtgcac cgcctccagc ggcgtgaagc
     7201 cgccgatcgc ttgcggttcg ctggaggttc cctggtagtg gtcgaagtag cagttcgaca
     7261 tcgggcacat gacgacgtcg tgtcccgcgc gcgccgcggc gcttcctttg gcgacattct
     7321 gccaggccat gatcaccgcc ccttcgggcg ccccgccatc gagaatctca tcccagccca
     7381 ccacccgctt cccctgtgcc cgcaggaacc gggcgacccg gccgacgaag taggcctgga
     7441 gcgcatcttc atcggccagc ccgagagcgt cttttcgccg ccggcagaga tcgtgactct
     7501 gccagcgtgc cttcgggcat tcgtctcctc cgatgtgcag gtagggggag gggaacagat
     7561 ccgcgacctc gctgagaacg tcctccaaaa atgtgaaggc ggcgtcgttg cccgcgcagt
     7621 agacatcatc gaacacgccc cactggttcg atacctggaa cggcccgccg gtgcacgaat
     7681 actccgggta ggccgccagg gccgccgtgg catgcccggg cagttcgatc tccggcacca
     7741 cggtcacctg ccgcgcggcc gcgtagcgga cgatctcccg gatttcagcc tgcgtgtaga
     7801 acccgccgta gcgccgccca tccggctccg tccgccaggc cccgatttcg gtcagccgcg
     7861 ggtagcgccc gatttcgatc cgccacccct ggtcgtccgt cagatgccag tggaaaatgt
     7921 tgagcttgtg gaaggcgagc aggtcgatgt acctcttgat gaacgcgggc gggaagaagt
     7981 ggcggctcac atccaggtgc atcccccgcc agccgaaccg gggggcgtcg cggatttcca
     8041 tccccggcag cacgatttcc cgttcccccg tctccgccgg ccagagaagc tgccgcagcg
     8101 tctgcaggcc gtagaagaga ccggacggcg aggcggcgtt gacccggact ccgcgttcgc
     8161 cgaccagcag ccgatatgac tcggcgaccg gctccggccc ttgctccatg agcagatgaa
     8221 ttccgggtgg gtctgccttc atctggtccg cctccaccgg ccgccacagg gcctcgcctc
     8281 ccggccaggc gtcgctgccg acggcaatga cccggctgat ccgcgcatcg ttcggtgcga
     8341 aatggaccgg gatcggcgct ctcagccgca ccccgccgcc ggtcgggaga atctcggccg
     8401 ggcgcgggat tacgtgtatc gggaaagtca ccgcggctgt ctccagccca ggagctgccc
     8461 ggagacccgc cagtagccgt aattgccccg ctcgtccttc gggcgaatgt cctcgatgac
     8521 gaatcggagc gtgtggcggc cggcggtgag gtggtcggtc agatccagct cgagaggcgc
     8581 cgcccggtct cccgggcacc agccgctgcg gctgtaatcg cacgaccagg aaccgtcggt
     8641 ccagcggcgg cagtacgggt tgatcgcccg gaaacggcgg cagtcatccc gccacggcgg
     8701 gtagcgatac accaccgcgc cgtcgaccga gatgacgttg tccttgctga cgaattcgtc
     8761 ggcatcggtg ccgtcggtgc agtggccgct gacatagtag tgcaagagca cgcgctccat
     8821 cccctcgggc acttcgatca ccgctgctct cccggccgct cccggatcgt cgcgcgtgaa
     8881 cgcctcctcg tagagcagcc cggccgccca gtcggcgttg agcgcctgtg aatccggcgc
     8941 gtaggtcagc gaaaaatcca tgcgccaacc cggtgtgacc cacgtgtcga cgaatccctt
     9001 gatcacgcgg gggccggcca gcagcggcgc caggggagac aggtcgacct cgaattcggt
     9061 cggcccccca tgggcagtca cgaacttgat cagttccaca tccgggccgc cggcgacctc
     9121 cagccgcaca tttccggcgc ggtcccagcg gtcgtacatc tcgagctcgt ctttcgggat
     9181 cggcctgagg gcgaggcgcc cggtgatccg taccgggccg gcgaacggag ggagctcgac
     9241 ccgggcggcg atcacgcgcc ccttgtcccg gacggtcacg ccgccgctct cgtacttcgc
     9301 agagtcgtcg ggcgcgaaat gaatcgtgtg gtctcggaag acgacgatgg tccgggcatc
     9361 cgccgtttcg aaggcgcgcg gagcgcccgc ccccgggttc aaaagcgcca gcgcggcgag
     9421 tacccccacg gtgaatagct ccgtcttgct cgccgttggt cgtctattgc gctgtgtcat
     9481 aggttaacct caaagtgcgt atctcaaacg gcgccatccg gagcaccgcc gccggcgccg
     9541 cctcaagccg tcggccgacc gcccgctcca tcaaatcggt ttcttcgaga gtgaaattcg
     9601 ggaagtcaat ctccagccgc ccggtcgagt gtcggccgtg ggtctccgcc acgcggagaa
     9661 tcaggccggc gtccttctcc gctttcttta ctgtctccag gaccacgnnn nnnnnnncgc
     9721 cctcgccgga gagccgcacc gggaggcggg tgttcgtcgc gcgcacgccc ttcgccgcca
     9781 gaagcccctg gttcagccgc gccgcctcgg ccatgacctc ggaggcggcc agatcaccgg
     9841 ggtgagggag gaggctgtag gtgaaccggt gttccccctg gtcggcgtcc ggatcggggt
     9901 aattgggggc gcgcagcagg ttgagatcca acaggccgtt ctcgacccgg tagccgtact
     9961 tgcagtcgtt gaggagcgcc accccgtggt cgcggtcgga cagatcggcg tagcggtgcc
    10021 ccacgacttc aaaccgggcc tgctcccacg aggtgttgcg gtgggtggcg cgccggagat
    10081 agccgtactg gatgtcgaac gcggcgtagt ccgagcggac cgtgaccggg aacgccacgc
    10141 gaagcatccg gtgcttctcg cgccagtcga ccgccgtttc gaaatcgagc cgccgcgaca
    10201 tccgccccag ccgcaccgtc tgcacaatgt gcgaccggcc gatccggtag cggaagcgaa
    10261 ccccccggcg cacgtccccg gccggcagcg gttcggccgc ggccgcccgc gccgtggcca
    10321 gcggcgtctc gcgatagaag gcatcgatat cccaggcgtc ccagtcgttc ggccgatcct
    10381 cgtacagtgt gagaatattc cccggcgcct cggggaccac cagttcgagg ccgagcgcct
    10441 tgtcgaaaca gcgcagcagc gcaccgctct ccgccaactc gtaccgcacc aggtcatttt
    10501 ccagcaccag actctggccg ggcggcgccg ccggggctgt tgtttctgcc gtccggcgga
    10561 gcgtcagcgc agcgtacgga ggaatgctca cgtgcaccgc cacgccgtcc ggttcccgct
    10621 ggcacggagg cgtcggcccg ccgccatcga tttcgcagcc cgcccagccc gccgggagtg
    10681 tgaccgcccc ctcgtaggga tagtgcaggg tattgaccag agtgaggcag ttcccgtcgg
    10741 ccggatacag ctccgcggcc accgcggcgg ccagccgttc gcacccggcg agggcctccc
    10801 ggtactccgt gtgcgccacc cggtagacgc ggccgatgct ggagccgggg atgatgtcgt
    10861 ggaattggtt gaggagcacc gtcttccaga ccgcgtccag ctcctgctgc gggtagcggt
    10921 cgagcgggag cttggccgcg agcatctcca gcgcccggag cttgtgctcc aggcggcgat
    10981 tggcccactt ggcatacgcc tgcgtcgtca aggtcccgcg gtggagttcc aggtacagct
    11041 ctcccgtcca ggtcggcagc cggtcccgtt cggcggcgag gcggcggaaa aactcgcggg
    11101 cggggccaaa ccgcacgcgc ggtgctccct ccagatcggc ctgccgccgg cccatttcga
    11161 tgtgctcctc tttcgggccg cccccgccgt cgccgacgcc aaagagacag aggaattcgt
    11221 cgagatagtc gttttcccgg aaacgctcct cggccgcggc gagcgattcg gggtgcagtt
    11281 ggctgttgta cgtgttttcc ggcgggaaat gggtcagcac ctcgctgccg tcgatcccct
    11341 gccaccggaa cgtgtggtgg ggaaattcat tcgtctggtt ccacgacagc ttctgggtga
    11401 ggaagtagtc gatgccgctc ttgcggagca gttgcggcag ggccgccgag tatccgaaga
    11461 cgtcgggcag ccagaggttg tcgacctcga cgccgaattc gtcgcggaag aagttcttgc
    11521 cgtggaggat ctgccgcacg agggattcgc ctccggtcac gttgcagtcc gcctccaccc
    11581 acattccccc ctgcggctcc cagcggccgg ctttcaccgc cgccctgacc tgtgcgtaca
    11641 gctcggggta gtgttctttg acgaaggcat agtgctgcgg ctgggaggcg ccgaacacgt
    11701 agtcggggta ccgcgcgagc aggtccagtt gggtggcgaa ggtccgggcg cacttgcgga
    11761 tggtctcacg caccggccag agccacgccg tgtcgatgtg ggcgtgcccg accgcggtca
    11821 ccgccaggct cgaggccgcg gccggttttt tcagttcgcg cgcgagtatc tcccggaatg
    11881 cccgggcgct ttcgccgctc gtcgcatacg cattcagccc gtcgaccgcc gcgcggacga
    11941 ttcgcgcacg gcgcacgctc ctctccggaa ggctcctcac cagcccgagg agcacgcgca
    12001 ggtcgagcgt gaggtgccac agctcggtgt cgaagacggc gaggcggagg gtgttgacgc
    12061 gcgcgtcgta ccggccgtcg cggttggccg cgtccgggcc ggggtcgaga tcgcagaaca
    12121 tgccaaacag gccgttggcg gccgcctcca cccagagctc gaccggttcc ccgcccgcgc
    12181 agggatcgaa gaggcggaca acatcccgcc caaattcgga gtcgaacacc gagccgttcg
    12241 tgatcccctg gagcatccgg ccgtcgggcc ggtacaccaa tccctcgccg cccaggtcga
    12301 tgtgcgccac gactgtccgc ccgcggtacg tctccggcac gcggccgacc agatgaaacc
    12361 agccgctctc ccagttccgg ccccagacat ctccgaccgc caccggctcg caggcgagcc
    12421 cgtgtctctg cgcgaacggc accggctcgg ccgagcgcgc acaggtcgcc gcgagcgccg
    12481 ccggctcgcg gaggattccc cccacggccc ggcgatagaa ctgctcgagc cttcggatcg
    12541 cgatgagcgt gtctttcgtg ttcactgtct gtctccgttc ggctttacgt gcggggggcc
    12601 ggccccgatt cggacacgaa cccgtcgtcg cgatggtcgc ggtcggccac cttcaggcgg
    12661 cggttcattc gccggaggtc ccggaaaccg ccgatcacaa accagatgat gatggccgac
    12721 gagaccgcca cgtggatggc gaggtaggtg cgccagaacg acagccaggc gccgtcgctc
    12781 acctcatggg tcagattgta gattgtgccg acgataaaca cgagcagcca cgcgccggtc
    12841 cagacgtagt tgaggatgta gatgatcttg tcgccgcgcg tgaactcccg tcccatcccg
    12901 agcatcttcc acccccgggc gggggtcgcg ttgacgaccg tgcgttctcc ctggatcgcg
    12961 tatcgcccgc ggtggagcag ccggtcgatg ttgaacggtt cgcggcggtt cagaagcgag
    13021 accgcgatgt agacgagcga ggcgcccagc atgctgatcc cccagaacca ctggccgttg
    13081 atgaagaaat cggggatgat ctggtggatg atgatcccgg ctacggcgat cccggagccg
    13141 gtaatcatgg cggcccaggc ggctgccgcc gtcccccgct tccagtacag cccgccgata
    13201 atgaccgccc ccgagccgcc ggcgaagatg gcgccggtga tggcaaagaa caggaagatg
    13261 tactcggcct gcgtgaagat gaggctgaag aagaaggcga acgcgccgac cgcgacaatc
    13321 gcccagcgca gcacgcggag atgctgtgcc ggcgcgaacg gctttttccg caggggcatg
    13381 atgacgtcct gcacgatgat gctcccccac gagtgcatgt aggcgtcgag cgtgctgatg
    13441 ctcgccgcga gcatcatggc ggcgaaagcc ccgatcagcc cgcgcggcag caggtgggtg
    13501 agcacgagcg gcactttcac ctggctctgc accgccttgc cctcgatccc cgacagggtg
    13561 tcggacaccg ccgctgcaat cgacgcaaaa tcggggtggt tgagaaccgt gtaggcgacg
    13621 atcggcacga acaggaacat gatcgacatc ggaaggttgc gcagcgacga gagcacgctt
    13681 gccatcttcg cctcgtgcgc gctccgggcc gacgaattgt aggcctgcgt cccctgccac
    13741 gacatcgtcc cgtagatcac gccgatgatg cccacgagga agtaccagaa attgaaatct
    13801 tccacctggc tcgtcttgaa cgggttgatc agcgaggcat cccgcggcgc cgccgccagc
    13861 gcctgctcga tgtgccccca gtcgaacgag acgaagaagt acacaacgag ggcgatgaac
    13921 acgccgtaga cgagcacgcc ctggaagaag tcggcgtaga tgaccgatac ttgtccgccg
    13981 ccgaggacga agtagagcga gacgcccagc aggaagagca tcatgagcgg gaagaggggg
    14041 acctcgaacc cgagcaggga gaccgattcc ggcagaccgc agaagtagat gaagaaccgg
    14101 gcttcgacgg ccgggaagat tccgaaattg acgatgccgg agaggaaggc gatgaagccg
    14161 gtgaagatgc ggaactcccg gctgtagcgg gcctcgaaat actgcgccag agtcagcgag
    14221 cgcgtctcgc gaaaccggta gatcacccat cccgacacgg tgatcaagag taccacgagc
    14281 gccgtcgtca tcccccacca ggtgagcgag aagccggcga tgaggttcat ctcgaggttg
    14341 ccgacgaccg tgattgcgcc gatgccggcg gcgccgctgg ccacgcacag gacataccgc
    14401 ccggcggagc gcccggccga gaggaaatcg gccacgctcc gcatcacccg ccgcccgccc
    14461 tccaccgagg cgatcatgag cccgagcgac acgatgacga tggcccagtc gagaaggctg
    14521 agattcatgg gtgtccgcct tgcctagccg cccgcctgcc cggtcggcgc cgtgcggccg
    14581 tcttctgtaa tccggagcgc cccgccgccg tcgcggcgga caacctccgc ttcaaaacga
    14641 tcttccgttg cccggagcgc cggcggccag gtgaaaccgc gcggtgcggc ggtctcgagc
    14701 gcgagatccg agtgggccgc ggtgaagcgg ccgtgcgcgg cctggtagtt gcgctcggcg
    14761 tagtacacct ggcggagcgc ccagcgggcg gaatccgccg ggtcggcgac cagggccgcc
    14821 tcgggcgacc cgggcggcgc cgccgaaaac tgcaccagcc cccacatctc cgggt
//
LOCUS       DAOPOE010000081        14873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4751_length_14873_cov_4.321096, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000081 DAOPOE010000000
VERSION     DAOPOE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4751_length_14873_cov_4.321096"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKM94_10505"
     CDS             <1..337
                     /locus_tag="PKM94_10505"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOD67103.1"
                     /translation="HSGLAAYIGESDLRAVEKRIEDGDEKALRYFTAMAYQIAKEIGV
                     AAVALEGQLDAIILTGGMAHSARLCGEIRRYAGFLGEFRIVPGEFEMEALAAAGVRFL
                     SGREPLKEY"
     gene            360..1313
                     /locus_tag="PKM94_10510"
     CDS             360..1313
                     /locus_tag="PKM94_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOD67104.1"
                     /translation="MEMIPIHSSGALIERAIAIAREGRRKTVAVAAAQDADVIEAAAQ
                     CRAAGMIEAVLIGDEAKIRALAREHGLDLGGCEIVHMPDPGQAAHCAARLASERRADA
                     IMKGFLPTSALLKVVLDKAYDLRGPNTLSHCAVLDIPGYHKLLNLTDGGMVVKPEPDQ
                     KLQILENAVRVGRALGLAPVKVAVSGATETPGEGAPQRLSDLDYVIPRAVERFRDVVI
                     QGPLTLDAAMSKEAAERHGMAGEVVGDADVFLVDTIEECNVVAKALVNFAGAVFAGVI
                     VGAKAPVSLVSRTDTATNKKASLAIACLLADYYAQQGVWES"
     gene            1320..2216
                     /locus_tag="PKM94_10515"
     CDS             1320..2216
                     /locus_tag="PKM94_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOD67105.1"
                     /translation="MEQVVTQTQARLGGRALRAAEIAPGRGEVIAACGRAVAAGLIAP
                     AVIGDFRQIARAEADAGCTLGGIRRVEAATAEEAARTAARMAAAGEIDLVIRGGDAPD
                     ILRVLLAEGAGLVRPGGTAAHVAMIKPEKYARAFLLTDALAHPQPDLKLKAALIETLV
                     RVGRRVGIETPRVAVLAAVEVVYPQMPVTLEAAVLSKMAERGQIKGAHVDGPLSFDCA
                     VDPAAAESKGIRNSAVAGRADAMLAPNIETAQGVYKAMALCGRAQMGGVLVGGRVPVA
                     MPLAGDGADTVFYSIVLACAVG"
     gene            2256..3419
                     /locus_tag="PKM94_10520"
     CDS             2256..3419
                     /locus_tag="PKM94_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67106.1"
                     /translation="MIVPVGILAAGVLLLQMQAGAHQSVQQHGEAAARSDSLAHCWDL
                     ERNARRVIPRLYWDGKTDSLRLVVAGVREKCPQASFDNLVVLLSIDDGTYDECLCDSE
                     MIAEIWWGFSRYGAVRYPSYNEHLLSQIPAVREWSEFTAQYIDDLKTRVDSNSTAFVQ
                     LQYYVDNVRYILRRLARSELPGTCLQDTYTSSIEALLQTRYRRRVHVYGRTGIWIPVG
                     GNKVLGNKLEVGGQFAVRDNRWECGLDFAARFLGESEPYRVRAGGEIRQTKEFLGLFV
                     GCSTAYEVIRARRWSADVFATVGFDGIRAVSRTGRNEAEWINSPALGLGFTHRVCFDR
                     GCTRYLGLQWRCTIVDYVTHGGSDLSGNALSLSLVWGAMSSGADRHLQRLQYF"
     gene            complement(3665..5686)
                     /locus_tag="PKM94_10525"
     CDS             complement(3665..5686)
                     /locus_tag="PKM94_10525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOD67107.1"
                     /translation="MRKAPRKSPSRKRPIAAEDLYRLRIPTSVAISPDESRVAYTVER
                     MDKDDLKYYSNLFVGDLRSGAQRQYTFGKTADGSPLWSPAGDRIAFISTRDKKTGIYL
                     IGAAGGAERKVIDLDGALDSLQWAPDGRHLVFALRWNDSHFIEKEEDKKKPPVFRHIT
                     RLYYRLDGSGFVPKDRFQVYTLDVESAALRRVTGGRRDNTQPAVSPDGRWIAYISNRR
                     PDPDVDMLYDDLFVIPFGGGREHRLPTPAGPKAAPSFSPDSRLIAYIGHDNPRDAWGV
                     TNHHIWTVGRTGSPKAVDLMPAFDRMALDMTISDLAEGHGVAVPRWSPDSRRIFFIGS
                     DTGRTSVYFVPARGGQPTRVFDGKCHVKALSLAAPARTAAIIYSDLATAGEIVTFPAV
                     HGGEKRAVTRTALNPFLVSEVVLGKTKDLMFRSFDGTAVQGWIVYPPAFRPNRKYPSI
                     LEIHGGPRMQYGYTFFHEMQWLAAQGYVVFYTNPRGGQGRGQTWADAISGGWGGLDYQ
                     DCMAAADFLAAQPFIDPKRMGVTGGSYGGYMTNWIIGHTDRFKAAVTQRSVVNFMSMY
                     GASDIGWSLERELDGTPWNNSGNYEKCSPITYFTNVRTPVLILHNEQDLRCGIEQAEQ
                     MFTMLKRLRRTVEMVRFPEEPHGLSRHGRPDRRIARLTWIARWFDRYLK"
     gene            5949..8243
                     /locus_tag="PKM94_10530"
     CDS             5949..8243
                     /locus_tag="PKM94_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD67108.1"
                     /translation="MEVLALIEQVRKAEPRKGARPKDTEPLEEYSGKTPAQLRRMLRT
                     MLLARRIEREEKLLLRKGYNRFFIGCGGKELMDVCLAEALRPNDPWIGYYRNKAFDLH
                     RGASIREKMLEAIGDARGQAGGMQIPAHPSYPQFAILPQASPTGSHALEAAGLGSAVK
                     WPLPISAQSFFPGGAYPQDAVVTCCIGEGSTSGNEFARAVFYSVFDKARNIYAIYNCG
                     WAISVSVEEQFPEGDPTTPFEGFKRFGLKIMQVDGTEIKEAIRGAEEMAAYARSGQGP
                     VLANIRVTREGSHSGSDDQSFYMDPVELDWHTYNDCILKTCNTLIADRIITPKEIGEI
                     WDELDKEVSEQSREAVKGYCPKTTEFIASKVYASTWDKTQETWRKYREASRVDRAAKY
                     REYHAKKYFATPELPETIGPMTMRFAINYTLFDLFGMTEDVLLFGEDVADFSGHMIHE
                     VEKQAALKGKGGVFLVSKNLQREFGNERCFNTPLDEAGILGRAIGHCYQGRRPIPEIQ
                     FLDYMSPAYQQLKERIATTYQRSNGQWTMPMVIRTTYGGYKQGAGAFWHSEGNLGTWA
                     NIPGLLIAVPSNAWDAAGLLRTAWACDDPVLFCESVAIYNRRDWEGVPVETPIPPIDE
                     LIPFGVAKVYDEEHSDVGIITYGATVHICRKAAELLKEKGIHVRIVDLRTVKPMDEEA
                     IRRTARECGKVCIVTEDRFHGGMAATISAVITGGEALYSLEAPLRVLTAIDSRVAYGM
                     DGDAACLPTVEKVAAMVEDLHDNF"
     gene            complement(8338..9837)
                     /locus_tag="PKM94_10535"
     CDS             complement(8338..9837)
                     /locus_tag="PKM94_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOD67109.1"
                     /translation="MAALRRQLIRNAFSSWFAYLVRIAVTFLFVPYIAAVLGGSRYGV
                     WVIIFQTIFYFSLLDAGLTSALTRYVSKFLAARDFERINRLLNTANLLYFFVGALVFL
                     GIFGFVELFFDYFRISDPETVAEGKTALLILGAFLAFNFIALPFGNTLGAFQRYDLAN
                     ALAVGEEVVRTLMMVYLLSRGYGLVALALAILVMTVVRHLLGAGLLLQRYPELDIGRR
                     FVEKPSARLLFGYSKISFGISLCWLILFNTDAFLLGLIGSAALAGVYHPGAQLMRYLR
                     NLINAAALPLIPAVSHKDAEGDFGPVRDLYRKSVRYVSYVSFALAAAVVVYARPFVDL
                     WLPPEFAEAAGVMQVLVVGAAFLLPQIVGHAILFGIERHGRLLVVLIIEAALKLGLSI
                     YLIPRYGLVGMAAAVAGPQVLVYTTLSPLFVGRELGISYVRTMGLTLASALAAVAIAG
                     PAAWLARWAIEPLSWTQFFVGLGVVGICALAGAWLIAEREDREQVRAWL"
     gene            complement(10038..10436)
                     /locus_tag="PKM94_10540"
     CDS             complement(10038..10436)
                     /locus_tag="PKM94_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67110.1"
                     /translation="MNKVVVRFADGRVVKGTTADFVPTKDLFHVNVMTDPVGAKPMEI
                     HTRDLKALFFVKDFTGDARHVHSNEFDPSRPPAGRKIRVEFKDGEILVGTTAGYQPGR
                     PGFFLMSADSDSNMERCYVVTAATKKVSFI"
     gene            10816..11982
                     /locus_tag="PKM94_10545"
     CDS             10816..11982
                     /locus_tag="PKM94_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67111.1"
                     /translation="MSGVNRVSAFPQTRPGTGGLRAAGAAALVVLSVLSAAGVRAADR
                     AELYGFAPAYPAAPLPPVAVERNQPFLTHKRLVRMVLEIDASGRVTGAAAADSTLGQF
                     ANYLAEYLSALPFVPAHFEGQAAASRLPVDFLFEPMRPAPEALPAVDHTGRVVEAGRY
                     FDTFPLNGIRPPRIVEFPSLFADLHWSDTASVYPYVLLALEIDSAGRVTAAEAVRSTL
                     PSFTQTTLNAALWATYDPLVVRGERRAGRCFVLAAYYPQVIYPTKVWRAEASAGAPIL
                     ERTRVRLMPDTVGLLAPPVPRLPTGDSLDVPGARVYSDRDTSGAVFAVDPSGRVSVQA
                     FDTNSRELTAALRRMAEHLKFYPALTLAGGRESFRGMVFVTGLPSGRLRINYVW"
     assembly_gap    12316..12325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12326..12506
                     /locus_tag="PKM94_10550"
     CDS             <12326..12506
                     /locus_tag="PKM94_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67112.1"
                     /translation="TAINLLLAATCFEVSFGWLLSAVVRGAIGLAVFLYFGLALGQMV
                     SVMERNPTVWSGMDA"
     gene            12583..14502
                     /locus_tag="PKM94_10555"
     CDS             12583..14502
                     /locus_tag="PKM94_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67113.1"
                     /translation="MEATIYTRACVWMLVVAMLATLGNPAAAEELKPEIQQMLNAGDT
                     AKAIEQLRAQIGLDNAYHYNYYILGMIYFNQERYEQALEQFQLALDRKTGHIASWYQL
                     GLTNLALDRLDEAQRAFEEGRKKAGKDRPEMFDNGLGLVAMARKQYLEATKLFQQATV
                     KDSLNASYWINLGDAYFNQGVAPLAVTYYQKALTLDTASTEVYYHWAEACLELRDYSC
                     AMDKLRTVLQSDSTHAPAWRRAGEIYFKAGLSSRVRADRMERFRETVGSYRRFFELSQ
                     AQPDSAHVRPYFELGMAYLNLNAFDSAIVYFDKVLAIPYEPRDIYYYYGRALWGAQDY
                     VRGGDFLTKHLAWVEEQGDGYTSSVNEAEFYQMLGDCWFYREHADEGQKAADYTTALG
                     YWKRSLALDSTQARIVYYTAVGYHTLGSYDQAMTYYDKRIALGIDSSNAAVYKNAGFC
                     ALNLAEQAGGESGGAEEFVEPGTAPADPVDPTVMYRRGAEYMLQYMQYQPADTAVAKR
                     VAYVYLYNLGDCENGVKYYQRVLQLDPGSCEALKSLGYAYFGGVCTKDYTKAIEYLTR
                     ANQCFSAQKACADVTTMLYIAQAYHLRAAAKLDARQDGTSDFEQANKWYGQVLKCDPN
                     NAEAKKGRDETQFEF"
     gene            14544..>14873
                     /locus_tag="PKM94_10560"
     CDS             14544..>14873
                     /locus_tag="PKM94_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67114.1"
                     /translation="MAEKVKQQDTPRQVSEDQRFKFIGFEVYPGKPKDLFGSEAERTK
                     LVEAIREKRRKGQVTREECKLFEERVTWIDKIVLTLACAVMIIALFFPWFAVYNEVVD
                     TAAAPREA"
BASE COUNT         2660 a         4365 c         5344 g         2494 t
ORIGIN      
        1 gcactccggt ctggccgcct acatcgggga gtcggacctg cgggcggtgg agaagcggat
       61 cgaggatggg gacgagaagg cgctgcggta tttcaccgcc atggcctacc agatcgccaa
      121 ggagatcggg gtggcggcgg tggcgctcga ggggcagctc gacgcgatca ttctcacggg
      181 cgggatggcc cactcggcgc ggctgtgcgg cgaaatccgg cgctacgccg ggttcctcgg
      241 cgagttccgc atcgtgccgg gggaattcga aatggaggcg ctggcggccg cgggcgtgcg
      301 tttcctgagc gggagggagc cgttgaagga gtactgaggg ccgggtccgg ggtgacggta
      361 tggagatgat cccgatacat tcgtccgggg cgctgatcga gcgcgcgatc gcgatcgcgc
      421 gcgaagggcg ccggaaaacg gtggccgtgg cggcggcgca ggacgccgac gtgatcgagg
      481 cggcggcgca gtgccgtgcg gccggcatga tcgaggccgt gctgatcggg gacgaggcga
      541 agatccgggc gctggcccgc gaacacgggc tcgacctggg cggctgcgag atcgtgcaca
      601 tgccggatcc cggccaggcg gcccactgcg ccgcccgcct cgcctccgag aggcgggccg
      661 acgcgatcat gaaagggttt ctcccgacct cggccctgct gaaggtggtg ctcgacaagg
      721 cctatgacct gcgcggcccc aacacgctct cgcactgcgc ggtgctcgac atccccggct
      781 accacaaact gctgaatttg accgacgggg ggatggtggt gaagccggag ccggaccaga
      841 aactgcaaat tctcgaaaac gcggtgcggg tgggacgggc gctgggactg gcgccggtga
      901 aagtggccgt gtcgggcgcg accgagacgc cgggcgaggg cgcaccgcag cgcctctccg
      961 atctcgacta cgtgatcccc cgcgcggtcg agcggtttcg cgacgtcgtc atccaggggc
     1021 cgctcacgct cgatgcggcc atgtcgaagg aagcggcgga gcggcacggc atggcgggcg
     1081 aagtggtcgg cgacgccgac gttttcctgg tggatacgat cgaggagtgc aacgtggtcg
     1141 ccaaagccct ggtcaatttc gccggggcgg tattcgcggg ggtcattgtc ggggccaagg
     1201 cgccggtgtc gcttgtgtcg cgcaccgaca cggcgaccaa caaaaaggcc tcgcttgcga
     1261 tcgcctgtct gctggccgac tactacgcgc aacagggagt gtgggagagc tgagcggcga
     1321 tggagcaggt tgtcacacag acacaggcgc ggctcggcgg tcgggcgctc cgagcggcgg
     1381 agattgcccc tggccggggc gaagtcatcg ccgcctgcgg gcgcgccgtg gcggcgggtt
     1441 tgatcgcgcc ggcggtgatc ggcgattttc ggcagatcgc gcgagccgag gcggacgcgg
     1501 gatgcaccct cggtgggatc cggcgggtgg aagccgcgac cgccgaagag gcggcgcgca
     1561 cggcggcgcg gatggcggcg gccggcgaga tcgacctggt catccgcggc ggcgacgctc
     1621 ccgacatcct ccgggttctg ctggcggaag gggcagggtt ggttcgcccg ggcgggacgg
     1681 ccgcgcacgt ggcgatgatc aaaccggaga aatacgcgcg ggcctttctg ctcaccgacg
     1741 cgctcgccca cccgcagccg gacttgaagc tgaaggcggc gttgatcgag accctggtgc
     1801 gggtcgggcg gcgggtgggc atcgagacgc cccgggtggc ggtcctggcg gcggtcgagg
     1861 tggtctaccc gcagatgccg gtgacgctgg aagcggcggt tctctcgaaa atggcggagc
     1921 gcggacagat caaaggggcg cacgtggacg gcccgctgtc gtttgactgc gccgtcgatc
     1981 cggccgccgc cgagagcaag gggatccgga attcggccgt ggccgggcgg gcggacgcca
     2041 tgctcgcgcc caacatcgag accgcccagg gggtctacaa ggcgatggca ctgtgcggcc
     2101 gggcgcagat gggcggcgtt ctggtcgggg ggcgcgtgcc ggtggcgatg ccgctggcgg
     2161 gggacggggc ggacacggtc ttttactcga tcgtgctggc ctgtgcggtg gggtaggggc
     2221 atagcaagcc gttacagcag aaggaggggt acggcatgat cgtgccggtt ggtatccttg
     2281 ccgcaggtgt tttgctgctg cagatgcagg cgggagcgca tcagagtgta cagcagcacg
     2341 gggaggcagc ggctcgctcc gattccctgg cgcattgctg ggacttggag cgcaatgctc
     2401 gccgcgttat cccacggcta tattgggacg gtaagacgga ctcgctgcgc ctggttgtcg
     2461 ccggtgtcag ggagaagtgt ccgcaggcaa gttttgacaa cctcgtggtg ctgctttcga
     2521 ttgacgatgg gacatacgat gaatgtttgt gtgattctga gatgatcgcc gagatctggt
     2581 gggggttttc ccgatacggg gccgtccgct acccgagcta caatgagcat ctcctgtcac
     2641 agattccggc ggtgcgcgag tggagcgagt tcactgcgca gtacatcgac gatttgaaaa
     2701 cccgtgtgga ttcgaatagc acggcgtttg tgcagttgca gtactacgta gacaacgtcc
     2761 gatacattct gcggcggctg gcgcggtccg agctgccggg cacgtgcctt caggacacct
     2821 acacgtcgag catcgaagcc cttcttcaaa cacggtaccg gcgccgagtc cacgtttatg
     2881 gcagaaccgg catttggata cctgtcggag gcaacaaagt tctcgggaac aaactcgaag
     2941 taggaggtca gtttgcggtc cgggacaaca ggtgggagtg cgggttggat ttcgctgctc
     3001 gcttcctggg agaaagcgaa ccgtaccgag tgcgcgcagg cggcgagata cgtcaaacga
     3061 aggaattcct gggcttgttc gtcgggtgct cgacagcata cgaggtgatc cgtgcaaggc
     3121 ggtggtccgc ggacgtgttc gctacggtcg ggtttgacgg catcagggcg gtttcgcgca
     3181 ccggccgcaa cgaagccgaa tggatcaact ctccggcgct cgggctcggt ttcactcatc
     3241 gggtctgctt tgaccgaggt tgcacccggt atttggggtt gcagtggcgc tgcacgatcg
     3301 tcgattacgt cacccacggc ggaagcgacc tgtcgggcaa cgcgctgtct ctcagcctcg
     3361 tctggggagc gatgtcgtcg ggggccgacc gccatctgca acgacttcag tacttttagc
     3421 ggtacagcga gtgttgggag agctgcaaga agagctgatg cagagaagaa cgcaccttgc
     3481 tctcagatac tgcacaaact gtcgaggccg tgcgcgggac cggagccgtg catgccccct
     3541 tggtcaggtc cagggagcgc attctgtata ctgatgcgct gttggcgggg gcggcagcgg
     3601 ttccatcaaa aaagccgtcg cctcgggcga ggcgacggct tggcagcatt cggacggcgc
     3661 cgaatcactt cagatacctg tcaaaccacc tggcgatcca ggtgagccgg gcgatgcggc
     3721 ggtcgggccg gccgtggcgc gagaggccgt ggggctcctc cgggaaccgc accatctcga
     3781 ccgtccggcg cagacgcttg agcatcgtga acatctgctc ggcctgctcg atcccgcagc
     3841 gaaggtcctg ttcattgtgg aggatcagca ccggggtgcg gacgttcgtg aagtaggtga
     3901 tcggcgagca cttctcatag ttgccggagt tgttccacgg tgtgccgtcg agctcccgct
     3961 cgagcgacca gccgatatcc gaggcgccgt acatggacat gaaattgacg accgagcgct
     4021 gggtgacggc ggccttgaag cggtccgtgt gtccgatgat ccagttggtc atgtagccgc
     4081 cgtaggagcc gccggtgacg cccatgcgtt tcgggtcgat gaagggctgt gcggcgagga
     4141 agtcggcggc ggccatgcag tcctggtagt ccagcccgcc ccatcctccg gagatcgcgt
     4201 cggcccaggt ctggccgcgc ccctgcccgc cgcgcggatt ggtgtagaag accacgtagc
     4261 cctgggcggc gagccactgc atctcgtgga agaacgtgta gccgtactgc atgcgcgggc
     4321 cgccgtggat ttcgagaatc gagggatact tccgattcgg ccggaaagcg ggcgggtaga
     4381 cgatccaccc ctggaccgcg gtgccgtcga acgacctgaa catgaggtct ttcgtctttc
     4441 ccagcaccac ctcggaaacg aggaaggggt tcagagcggt gcgggtcacc gcccgcttct
     4501 ccccgccgtg gaccgccggg aaagtcacga tctcgcccgc tgtcgcgaga tcggagtaga
     4561 tgatggcggc ggtgcgggcc ggtgcggcga gggacaacgc tttcacgtgg cacttgccgt
     4621 cgaacacgcg ggtgggttgg ccgccgcgcg cggggacgaa gtacacactc gtgcggccgg
     4681 tatcggagcc gatgaagaaa atccgccggc tgtcgggcga ccagcggggg acagcgacgc
     4741 cgtgcccctc ggccaggtcg gaaatggtca tgtccagcgc catccgatcg aacgccggca
     4801 tgaggtcgac cgctttgggg gagccggtgc ggccgaccgt ccagatgtgg tggttggtca
     4861 ctccccaggc gtcgcgcgga ttgtcgtggc cgatgtaggc gatgaggcgg ctgtcggggg
     4921 agaagctcgg cgccgccttg gggcccgccg gggtagggag acggtgctcc cgaccgccgc
     4981 cgaacgggat gacgaagaga tcgtcgtaga gcatgtcgac atcggggtcg gggcggcggt
     5041 tggagatgta cgcgatccag cggccgtcgg gcgacaccgc cggctgggtg ttgtcccgcc
     5101 gtccgccggt cacccgtcgc agcgccgcgc tctcgacatc cagggtgtag acctggaaac
     5161 ggtctttcgg cacgaacccg gaaccgtcga gacggtagta caaccgcgtg atgtggcgga
     5221 aaacgggcgg cttcttcttg tcctcctctt tctcgataaa gtgagagtcg ttccagcgga
     5281 gggcgaacac gagatgccgg ccgtcgggcg cccactgcaa gctgtcgagg gcgccgtcga
     5341 ggtcgatgac tttccgctcg gcgccgccgg cggcgccgat gaggtagatg ccggtcttct
     5401 tgtcgcgggt cgagatgaag gcgatgcggt cgccggcggg cgaccagagc ggcgagccgt
     5461 cggccgtctt gccgaaggtg tactggcgct gcgcgccgga gcggaggtcg ccgacaaaca
     5521 ggttgctgta gtacttgaga tcgtccttgt ccatccgctc cacggtgtag gcgacgcgcg
     5581 actcatcggg ggagatggcc acggatgtcg ggatgcggag gcgatagagg tcctcggccg
     5641 cgatcggccg cttgcgggaa ggagacttac ggggggcttt tcgcatgaga ggggcttcct
     5701 ttccaatgga gatcgggttg tcacgcggat actagcgttt aagccgctcc ggcacaagcg
     5761 gaaaagtcgc acgccccggg ggcggccgga ggggcggcgg gcgccgaccg gaaatgtcca
     5821 atttggttgc cttttcccga aaaagcgata tttttgggga ccaggcggcc cgcggccgcg
     5881 ttaatacatt gattaaacgg accaagataa tcgatgttta gacatagcca ttcaggaggt
     5941 tagcactttt ggaagtcctg gctctgatcg aacaagtgcg caaggccgag cccaggaagg
     6001 gggcgcggcc caaggatacc gaaccgctcg aggagtattc ggggaagacg cccgcgcagt
     6061 tgcggcggat gttgcggacg atgcttctgg cgcggcgcat cgagcgcgag gagaagctct
     6121 tgctgcgcaa agggtacaac cggttcttca tcggctgcgg cggcaaggag ctgatggatg
     6181 tgtgtctggc cgaggcgctg cggccgaacg acccttggat cggctactac cgaaacaaag
     6241 ccttcgacct gcaccgcggc gcttcgatcc gggagaaaat gctcgaggcg atcggcgacg
     6301 cccggggaca ggcggggggg atgcagattc ccgcccaccc gtcctacccc cagttcgcca
     6361 ttctcccgca ggcctcgccg accggttcgc acgcgctcga ggcggccggt ctcgggtcgg
     6421 cggtcaagtg gccgctgccg atttcggccc agtcgttttt ccccggcggg gcctacccgc
     6481 aggacgccgt ggtcacctgc tgtatcggcg aaggctcgac cagcggcaac gagttcgcgc
     6541 gggcggtctt ctactcggtg ttcgacaagg cgcgcaacat ctacgcgatc tacaactgcg
     6601 gctgggcgat ctcggtgtcg gtcgaagagc agttccccga gggggacccg accaccccct
     6661 tcgaggggtt caagcggttc gggctgaaga tcatgcaggt cgacggcact gagatcaagg
     6721 aggccatccg gggagccgag gaaatggcgg cctacgcccg gtccggccag ggccccgtgc
     6781 tggccaacat ccgcgtcacc cgcgagggtt cgcactccgg gtcggacgac cagtcgttct
     6841 acatggaccc ggtcgaactg gactggcaca cctacaacga ctgcattctc aaaacctgca
     6901 acacgctcat tgccgaccgg atcatcaccc cgaaagaaat cggggagatc tgggacgagc
     6961 tggacaagga agtctcggag cagtcgcgcg aggcggtcaa ggggtattgt cccaagacca
     7021 cggagttcat cgcgagcaag gtctacgctt cgacctggga caagacgcag gagacgtggc
     7081 ggaagtaccg ggaggcgtcc cgggtcgacc gggccgccaa gtaccgggag taccacgcga
     7141 agaagtactt cgccaccccc gagctgccgg agacgatcgg cccgatgacg atgcggttcg
     7201 cgatcaacta cacgctcttc gacctgttcg gcatgaccga ggacgtcctc cttttcggcg
     7261 aggacgtggc cgacttctcc ggccacatga tccatgaggt ggagaagcag gcggcgctga
     7321 aggggaaggg gggcgtgttt ctcgtgtcga agaatctcca gcgcgagttc ggcaacgagc
     7381 gctgtttcaa cacgccgctc gacgaggcgg ggattctcgg ccgggccatc ggccactgct
     7441 accaggggcg ccgccccatt cccgaaatcc agttcctcga ctacatgtcc cccgcctacc
     7501 agcagcttaa ggagcggatc gccaccacct accagcgctc gaacggacag tggacgatgc
     7561 cgatggtgat tcggaccacc tacggcggct acaagcaggg ggcgggggcg ttctggcact
     7621 ccgaggggaa cctcggtacc tgggcgaaca tccccggcct cctcatcgcc gtcccctcca
     7681 acgcctggga cgcggccgga ctgctgcgga ccgcctgggc ctgcgacgat ccggtgctgt
     7741 tctgcgaatc ggtggccatc tacaaccggc gcgactggga aggcgtgccg gtggaaacgc
     7801 cgatcccgcc gatcgacgag ctgattccgt tcggggtggc caaggtgtac gacgaagagc
     7861 acagcgacgt cggcatcatc acctacggcg cgaccgtgca catctgccgg aaagcggccg
     7921 agctgctcaa ggagaagggg atccatgtgc gaatcgtcga cctccgcacg gtcaagccga
     7981 tggacgagga agcgattcgg cggacggcga gggagtgcgg caaggtctgt atcgtgaccg
     8041 aggaccggtt ccacgggggg atggcggcga cgatctccgc cgtgatcacg ggcggcgagg
     8101 cgctctacag cctcgaggcc ccgctgcggg tgctcaccgc gatcgacagc cgggtcgcct
     8161 acggcatgga cggcgacgcg gcgtgcctgc cgacggtgga gaaagtggcg gcgatggtcg
     8221 aggacctgca cgacaatttc tgagcgcgag ggcgccggcg atgagagcga ggccgcggct
     8281 ccggcgggag cggcggccgc gctttttttc ggctcccggc gaagctggcg ccggccgcta
     8341 cagccaggcg cgcacctgct cgcggtcctc ccgctcggcg atcagccagg ccccggccag
     8401 cgcgcagatc ccgacgacgc cgaggccgac gaagaactgt gtccagctca ggggctcaat
     8461 cgcccagcgg gcgagccagg cggccggccc ggcgatcgcg accgcggcga gagccgaggc
     8521 cagcgtgagg cccatggtgc ggacgtacga gatgcccagc tcgcggccca cgaacagcgg
     8581 gctcagcgtc gtgtacacga gcacctgcgg cccggcgacc gccgccgcca tgccgaccag
     8641 gccgtagcgc gggatgaggt agatcgacag gccgagtttc aacgccgctt cgattatcag
     8701 gaccaccagc aggcggccgt ggcgctcgat gccgaagagg atggcgtggc cgacaatctg
     8761 gggcaggagg aaggcggccc cgaccacgag cacctgcatg acgccggcgg cctcggcgaa
     8821 ttccggcggc agccagagat cgacaaaggg gcgggcgtag acgaccaccg cggcggcgag
     8881 ggcgaacgac acgtatgaca catagcgcac cgatttgcgg tagaggtcgc gcaccgggcc
     8941 gaaatccccc tccgcgtcct tgtgcgagac cgccgggatg agcggaaggg cggcggcgtt
     9001 gatgaggttg cgcaggtagc gcatgagctg ggcgcccggg tggtagaccc cggccagggc
     9061 ggccgacccg atcagcccga gcaggaaagc gtcggtgttg aagaggatga gccagcacag
     9121 ggagatgccg aacgagatct tcgagtagcc gaagagcagc cgcgcgctcg gtttctccac
     9181 gaagcggcgg ccgatgtcga gctcggggta gcgctgcaaa agcagccccg cgccgaggag
     9241 gtggcgcacg acggtcatga cgagaatcgc cagcgcgagc gccaccaagc cgtacccccg
     9301 gctgagcaga tacaccatca tgagcgtccg gaccacctcc tcgccgaccg ccagcgcgtt
     9361 cgccaggtcg tagcgctgaa acgcccccag cgtgttgccg aagggaaggg cgatgaagtt
     9421 gaaggcgagg aaggccccga gaatcagcag cgccgtcttc ccctccgcga ccgtctccgg
     9481 gtcggagatg cggaagtagt cgaagaacag ctcgacgaat ccgaagatgc cgaggaagac
     9541 gagggcgccg acgaagaagt acagcaggtt ggcggtgttg aggaggcggt tgatgcgctc
     9601 gaaatcgcgc gcggcgagga atttggagac gtagcgggtc agcgccgagg tcaggccggc
     9661 atcgagcagc gagaagtaga agatcgtctg aaagatgatc acccagacgc cgtagcggct
     9721 cccgccgaga accgccgcga tgtaggggac gaagaggaag gtcacggcga tgcgcacgag
     9781 gtaggcgaac cacgaggaga aggcgttgcg gatgagctgt cgacgtaatg cggccatggg
     9841 gtatgttcct tgaacgggag tgtcggagat tcggccggcc ggcgcaagtt tgaaccgggg
     9901 gcggttgggg gcgcccgaac ggttcccggc gggcgccggg aggtcggggc gggcccggcc
     9961 ggtcccgggt cggcggccag agagccacgg gcggaatgag gccggcccgc ctggccgcgc
    10021 gctgcaagtg tcgcttttta tatgaagctg acttttttcg tggccgccgt gactacgtag
    10081 cagcgctcca tgtttgagtc tgaatccgca gacatgagga agaagcccgg ccgtccgggc
    10141 tgatagcccg ccgtcgtgcc caccaggatc tcgccgtcct tgaactccac ccggatcttg
    10201 cgcccggccg gggggcgcga ggggtcgaat tcatttgagt ggacatggcg cgcgtcgccg
    10261 gtgaagtcct tcacgaaaaa cagcgctttc aggtcgcgcg tgtgaatttc catcggcttc
    10321 gccccgacgg gatcggtcat cacgttcaca tgaaagagat ccttggtcgg gacgaaatcg
    10381 gctgtcgtac ctttgactac tctaccgtcg gcgaaccgta ccaccacttt attcacagcc
    10441 ttctcctctc gcaaaaccag gccgccacga cactcttcaa tccggcctct ctgccgtcag
    10501 cgattccacc accccaaggt ccgtgcggtc gccggacgtt tgcggtgcgt gagaatcacc
    10561 cgcatggtaa tccaaattga cgtgggcatc aagtggaaca acaccgccgc gtcaccggcc
    10621 gggccagagc cgggcagcgc gggcggggcg tgtcggggca gcgccgggaa cgttcgggga
    10681 ctgctgccgg cgaaggcggc cgaaaaggcc gccggcggcg gtgcagaaaa gcgttgtctt
    10741 ttgcagcgga cagccgctta atgcgtgtaa aaggtgaatt gaggcggcga gaaccctgag
    10801 ctgagggagt gaccggtgag cggagtgaat cgggtgagcg cgttccccca aacgagaccg
    10861 ggtacgggcg ggctgcgcgc ggccggggcg gcggcgctgg tggttttgag cgtgctctca
    10921 gccgcgggcg tccgcgcggc cgaccgggcg gaactgtacg gattcgcgcc ggcctatccg
    10981 gcggcgccgc tgccgccggt ggccgtggag cggaatcagc cgtttctcac gcacaagcgg
    11041 ctggtgcgga tggtgcttga gatcgacgcg tccggccggg tgaccggggc ggcggccgcc
    11101 gactccacgc tggggcaatt cgccaactac ctcgcggagt acctgtcagc gctgccgttt
    11161 gtgccggcac attttgaggg tcaggcggcc gcgtcgcggc tgccggtcga tttcctgttc
    11221 gagccgatgc ggccggcgcc cgaggcgctg ccggcggtcg atcacacggg acgggtggtc
    11281 gaggccgggc ggtatttcga cacctttccc ctcaacggca tccgcccgcc gcggattgtc
    11341 gagttcccct cgctgtttgc ggatctccac tggtcggata ccgcctctgt ctatccctac
    11401 gtgctcctgg cgctcgagat cgacagcgcc ggccgggtga cggcggccga ggcggtgcgc
    11461 tcgacgcttc cctcgttcac gcagacaaca ctcaacgccg ccctctgggc gacctacgac
    11521 ccgctcgtcg tgcgggggga gcgccgggcg gggcgctgtt tcgtgctggc cgcctactac
    11581 ccgcaggtca tctatccgac gaaggtgtgg cgagcggagg caagcgccgg ggcgccgatt
    11641 ctcgagcgga cgcgcgttcg gctgatgccc gataccgttg ggctgctggc gccgccggtc
    11701 ccgcgccttc caaccggcga ctctctcgat gtaccggggg cgcgcgtgta cagcgaccgc
    11761 gacacgagcg gggcggtttt tgctgtcgac cccagcggac gggtgagcgt gcaggcgttt
    11821 gacacgaatt cgcgggagct gacagccgcg ctgcggcgaa tggcggagca cctcaagttt
    11881 tatccggcgc tgactttggc cggcgggcgg gagtcatttc gcgggatggt gtttgtaacc
    11941 gggctgccta gtggccggct acgaataaac tacgtatggt gagaacggct gctccggccg
    12001 gggcgatgtt ttaagttgtt gcgatgtagg ggcttggcga ttgttgcggg acagggcggc
    12061 gggtatcggc tgacgcataa gttgcgttca tttcgcgggg taggctgccg ggcaaattgc
    12121 tatttcgtcc atcttgttgt ctcctaatat cttcccgctc acttcgggcg ccgggccgct
    12181 ccgggcatgg atgttgaaca tgcgaatcca ggaaacttct gtggaccatc ccctcggcct
    12241 ggaagaaaca gcgggcagcg gtgtagaaaa ggagagtgtg agatggaaag gacggtaggc
    12301 atgctgaaca tggtgnnnnn nnnnngacgg cgatcaactt gcttctggcg gccacctgtt
    12361 tcgaagtgag tttcggttgg cttctcagcg cggttgtgcg cggggcgatc gggcttgcgg
    12421 tgtttctgta ttttggtctc gcgctcggac agatggtgag tgtgatggag agaaatccga
    12481 ccgtgtggtc gggtatggac gcatagggaa atattcgctt gacaaggggt gatttgcacg
    12541 tatttttcga atctgtttag gaagttaatg agggaaaaag gtttggaagc aacgatttac
    12601 acacgcgcat gcgtctggat gctggtagtg gcgatgctcg ccacgctggg gaatccggcg
    12661 gcggccgagg agctcaagcc ggagattcag cagatgctca acgccggcga cacggccaag
    12721 gcgatcgagc agcttcgcgc gcaaatcggc ttggacaacg cctaccacta caattactac
    12781 atcctgggca tgatctattt caaccaggag cggtatgagc aggcgctgga gcagtttcag
    12841 ttggcgctcg accgcaagac ggggcatatc gcgtcgtggt accagttggg gctgacgaac
    12901 ctcgcgctgg accgcctcga cgaggcccag cgggccttcg aggaggggcg caagaaagcg
    12961 ggcaaggaca ggccggagat gttcgacaac ggtctcgggc tggtggcgat ggcgcgcaag
    13021 cagtatctcg aggcgaccaa gctcttccag caggcgaccg tgaaagattc gctcaatgcg
    13081 tcgtactgga tcaatctggg cgacgcgtat ttcaaccagg gggtggcgcc gctggcggtg
    13141 acctactacc agaaagcttt gaccctggac acggcctcga ccgaggtgta ctaccactgg
    13201 gcggaggctt gcctggagtt gcgcgattac agctgcgcca tggacaagct gcggacggtg
    13261 ctgcagtccg acagcacgca cgccccggcc tggcgccggg cgggcgagat ctatttcaaa
    13321 gccgggctct cctcgcgcgt ccgggccgac cggatggagc ggttccgaga gacggtcgga
    13381 tcgtaccggc ggttttttga actcagccag gcgcagccgg actcggccca cgtgcggccg
    13441 tatttcgaac tcggcatggc ctacctgaat ctcaacgcct tcgattcggc gatcgtctat
    13501 ttcgacaagg tgctcgccat cccctatgag ccgcgggaca tctactacta ctacggccgg
    13561 gcgctctggg gagcgcagga ctacgtgagg ggcggggact tcctcaccaa gcatctcgcc
    13621 tgggtggagg agcaggggga cggctacacg tcgtcggtca acgaggcgga gttctaccag
    13681 atgctcgggg actgctggtt ctaccgggag cacgccgacg aggggcagaa ggcggccgac
    13741 tatacgacgg cgctggggta ctggaagcgg tcgctggcgc tggactcgac gcaggcgcga
    13801 atcgtgtact acacggcggt cgggtaccac accctgggca gttatgacca ggcgatgacc
    13861 tactacgaca agcggattgc gctcgggatt gactcgagca atgcggcggt ctacaagaac
    13921 gccggtttct gcgcgctcaa cctggccgag caggcgggcg gcgaatcggg cggggcggag
    13981 gaatttgtgg agccggggac ggcgccggcg gatccggtcg acccgacggt gatgtaccgg
    14041 cggggggccg agtacatgct ccagtacatg cagtaccagc cggccgacac ggcggtggcc
    14101 aagcgggtgg cctacgtcta cctgtacaac ctcggcgact gcgagaacgg ggtgaagtat
    14161 taccagcggg tgctgcagct ggatccgggg agctgcgagg cgctgaagtc gctcggctac
    14221 gcatacttcg ggggcgtgtg cacgaaggac tacaccaagg cgatcgagta tctcacgcgg
    14281 gccaaccagt gtttcagcgc ccagaaggcg tgcgcggacg tgaccaccat gctgtatatc
    14341 gcccaggcct accacctgcg ggcggcggcg aagcttgacg ccaggcagga cgggacgagc
    14401 gatttcgagc aggccaacaa gtggtacggc caggtgctca agtgcgatcc gaacaacgcc
    14461 gaggcgaaga agggccggga cgagactcag tttgaattct aaggctgtgt attgtgtaag
    14521 gcgtctttct ggtaggagcg ttaatggctg aaaaggtgaa gcaacaggac acgcccaggc
    14581 aagtatcaga ggaccagcgg ttcaagttta tcggttttga ggtctatccg ggcaagccca
    14641 aggatctgtt tggaagcgag gctgaacgga caaagctcgt ggaggcgatt cgggagaagc
    14701 gccgcaaggg gcaggtgacg cgggaagagt gcaagctgtt cgaagagcgc gtcacgtgga
    14761 tcgacaagat cgtgctgacc ctggcctgtg cggtaatgat tattgctctg ttctttccgt
    14821 ggtttgcggt ctacaatgaa gtggtcgata ccgcggccgc gccgcgcgag gcg
//
LOCUS       DAOPOE010000082        14496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_4883_length_14496_cov_5.299218, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000082 DAOPOE010000000
VERSION     DAOPOE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_14496_cov_5.299218"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKM94_10565"
     CDS             complement(<1..518)
                     /locus_tag="PKM94_10565"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOD67115.1"
                     /translation="MAERARKAYGVLAEFRSTGDLLRAAEKLRDAGFRRWDVHSPFPI
                     HGMDRAMGLGRSPVGFIVGLCGTTGLLVFLAFLYWINVYDYPMVISGKPHFSYQAFVP
                     PLFAITILSSALAALFGMLALNRLPRLHHPLFSSPQFEKVTGGGFFISVEADDPKFAE
                     IERTGLLEALGAT"
     gene            complement(511..1854)
                     /gene="nrfD"
                     /locus_tag="PKM94_10570"
     CDS             complement(511..1854)
                     /gene="nrfD"
                     /locus_tag="PKM94_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOD67116.1"
                     /translation="MVGNPTYEEITEAVSRIPEQRATPKAWWVLFLVSAALAGMFFAH
                     VGYLFWKGTGIWGLNIPVGWGFAIVNFVFWVGIGHAGTLISAILFLLRQRWRTSINRF
                     AEAMTIFAVICALMFPTIHVGRVWFIYWTMPIPNQMGMWPNFRSGLVWDAFAVGTYFL
                     CSLMFWYVGLVPDLATLRDRAKGKIRKILYGVFSLGWRGGNRQWRHYELAYLVLAGVS
                     TPLVLSVHSIVSTDFATSLVPGWHTTIFPPYFVAGAIFSGFAMVMTLSLIARRVYGLE
                     HIISLKVLERMNIIMLVTGSMVGYAYGMEFFIAWYSGNIYEKFTFVNRAFGPYAWAYW
                     IMVTCNVITPQLFWFKRLRTAIPVMFVASILINIGMWFERFVIVMSLSRDFLPSSWDY
                     YSPTWHDVGLLIGSFGLFFTLFLLFLRFLPMVAMAEVKAVLPQADPHYHGERSHG"
     gene            complement(1905..4934)
                     /locus_tag="PKM94_10575"
     CDS             complement(1905..4934)
                     /locus_tag="PKM94_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOD67117.1"
                     /translation="MSSLKDDRSLTGRQYWRSLDQLTGTPEFEEWLHREFPQGASEWA
                     NAWSRRSFLTLMGASLALAGLSGCRRPEEKIVPYVQPPEDLVPGIPVTYATTMPWGTS
                     AYGILVESHEGRPTKIEGNPLHPSTRGAANAAIQASILDFYDPDRSAAFVREGVNTDW
                     GAFVTFWREQFLAFQAGGGRGLAVLAEPSSSPTIARLRRAFQQRFPNARWIAWAPVAD
                     ERRQEALRIAFGRDLQPVFHYDRAKVILSLDSDFLLTEDEMVAATHGFAAGRRTTSEK
                     DRMNRLYAVEGLLSVTGANADHRLRLRAREVGSFALALAAELARQGLPLEMLGGAKIP
                     QLSGAAAQWLAPLAGDLLRARGESLVVVGRRQPSAVHALAAALNEALGNTGRTITYVD
                     NPDVARSDSPGLAELVKGMASGEIQALVMLGGNPAYNAPADLEFRAALAKVPTTVHLS
                     THRDETSQAALWHLPRAHYLEAWGDARAVDGTLSLVQPLIEPLYGGKSDSEVLALLAG
                     GDDARGYDLVRETWRGLIAGDFENGWRQTLHDGILPGQTAAAVAATVRPGEIAAAIKA
                     HQWEVASADARGLEGIFHPCYTTGDGRQANNGWLQELPDPITKLAWDNVATLSPKTAE
                     ELGVKNEDLVRVAVNGRELTLPVWIVPGQADYCVGLALGYGREAAGRVASGVGFDTYR
                     LRTAAAMHAAPGVSLTPTGRSYELATTQDHGSMEGRPIVLEATLDEYRRDPDFAARAA
                     VVPAFMAESPKAQERELVREYPSSWQEPSYKDGHQWGMAIDLSACTGCNACTVACQAE
                     NNIPVVGKDQVRRGREMHWIRVDRYFEGDLEDPRVVHQPMPCQHCENAPCEQVCPVAA
                     TVHSAEGLNQMVYNRCIGTRYCSNNCPYKVRRFNFYHYTANYPETIRMQQNPEVTVRS
                     RGVMEKCTYCIQRISRGKRQARLENRTLRDGEVVSACQQACPAEAIVFGDINNPESNV
                     VRVKKSNRDYALLGEYNTQPRTTYLARIRNLNPEMPA"
     gene            complement(4922..5536)
                     /locus_tag="PKM94_10580"
     CDS             complement(4922..5536)
                     /locus_tag="PKM94_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD67118.1"
                     /translation="MPLLLAAAAIVIGAAAVLGVWYYGSPRYTDVGYRPVQPVAYSHK
                     LHAGDLGIDCRYCHTAVEQSRYATVPPTQTCMNCHKLILPESQKLLPVRESFATGRPI
                     EWVRIHKMPDYAYFDHARHVQSGVGCFSCHGNVAQMEVVEQVQPLSMSWCLSCHRNPA
                     PHLRPLDQITNMTWAPAPDHADWAERFMQEHHIRPSEDCWTCHR"
     gene            complement(5899..6831)
                     /locus_tag="PKM94_10585"
     CDS             complement(5899..6831)
                     /locus_tag="PKM94_10585"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOD67119.1"
                     /translation="MRGCRARAAGKVPPAERNRPMKATLAHYFQLTKPSIMLLVVFTG
                     TASLVIEGSLLDRPGRFLLVLLGLYLAGGSANALNQYFERNIDAQMKRTSGRRPLPRK
                     QISPAGALVFSVAIGVLGVAVFALFFNLLTAALALATILFYSLFYTLYLKPTTPQNIV
                     IGGIAGAMAPVGAWTAATGAMAVAPWIMFAIVFLWTPPHFWALALYCKDDYEKVDLPM
                     LPLVKGDRATVDRIFAYTLAVVGASLVLIAFTAGWFYFAVAALLGGQFIRRAARVRRE
                     QSVAGYRSLFGYSIVYLFSLFGAMMADGVLQRWL"
     gene            6978..9410
                     /locus_tag="PKM94_10590"
     CDS             6978..9410
                     /locus_tag="PKM94_10590"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOD67120.1"
                     /translation="MYAPQPVYRECTTRAACLFRRVRFALLPAAVALVLGAPRTAAAA
                     IIEGTICDSATHEPIPRVTIRVEHEDRVMFSNEDGYYRLRLDPGTHRLRFTHIAYFPA
                     ACDVALSDSTSSRDILLASCLIDVPGTRVYSRAYDPAQEIIVEAIRHKEELLARIRDY
                     RFEGYTKLAVRRLTDDGPAEYFAIIESQLEGWFRQPDRYKEIITARRQSANIPAEVNI
                     ITIGEIFNFNANRIDLGQPVVSPTASDALDYYNYYLLDTVLVDGAPAFLLEVEPRTDA
                     HALFAGRILIVDSVYAVAGVDLGLNKGFDSPYLKDLRFTQRMSRFDNDIWMPVEIRFD
                     GDFQSPLPLIPDLRFAYTGLLNRLVINQGIPEERFDRYVLEVAPEADDLDSAAWEQGA
                     LLPLTESEQTGYRYLDSLKHRPLTPRRALGYGVMLAAAGTMAQSAHKFFHFNRVEGPY
                     LGAGANLTKLVPRSELSLKTGRAFDADCWQHHYRLQVLLHEPTQLKAAIGYRDEIVAR
                     PNIYGDQALNPTFANLFFKYDPIDYYLEKGFDLGLSLRILPHTSATLGYRDFRQSTVG
                     NTTEYSLTGIDRAYRSNPVIADGRLRALSAGLAYDSRPLVRFKGREQQVMAYPYTSVR
                     LEFEAADPDLIATDFHFRRYALSLVRVQSLAGWGFTSLRAYAGGSDYALPPQRFFTVS
                     LENPYEPFLEFKTLSDFNVTGDRVAAFYLEHDFGGTLFRRLGLPLLEELPFGLAVYGG
                     SFWTDIRAATRASFPAAFAGAPDPPKSAPDWYSEIGFGISRLPLLVELLFTWRVSAHE
                     SDRFSFKFNMSF"
     gene            complement(9477..10958)
                     /locus_tag="PKM94_10595"
     CDS             complement(9477..10958)
                     /locus_tag="PKM94_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOD67121.1"
                     /translation="MIRGKYLTPMLVVIGLLVAGGIITEYFDYPNSVQAQNQRQAAGP
                     ADRPIQSLRDLGNAFVEIADEVRPSVVTVFSERVLRMRNPFYNSPFGGFFDQFFGTPG
                     RQAPEQEYRQTGLGSGVIISEDGYIVTNNHVVQDADTIYVQTSDGQRQTAKVIGTDPR
                     TDIAVLRIDANGLRAIRLGNSDDIRVGEWVVAVGSPLSPNLAQTVTQGIVSAKGRSNV
                     GLADYEDFIQTDAAINPGNSGGPLVNLDGELIGINTAIASQSGGYQGIGFAVPVNMVK
                     YVMESLIQEGHVVRGWLGVSISDISPAMSRTLGLESTQGALVQEVVKGSPAEDAGLKE
                     GDVILAIDGQTVQNSTELRNRIAEIRPGTRASLTVFHEGAKNQVWVKLVEMTAEAAAA
                     GGPGSSLDETLGFQVAELTRDLAREYGVEGGAGGAVVIAVDSESSAYQAGLREGDVVR
                     AVNRRQVRSVDELRSLLSGVGKGDDVLLRVQRQDRYFFIAFTL"
     gene            11160..12239
                     /locus_tag="PKM94_10600"
     CDS             11160..12239
                     /locus_tag="PKM94_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOD67122.1"
                     /translation="MRAIPAPALRAAFLMAAVALAVECSDDYKSTDNPEPGSPVLAAI
                     GPRSTTTDVPLAFTVTATDPDGQIPALSAVDLPEGASFTDHATGAGDFAWTPTLAQAG
                     THQVTFIASDGALTDQEIVTITVSEPGEQTAIIADHRAPERAAQIPGAILDRIRDSLT
                     IYFGHTSHGSQLLTGMDLLAGMSSAYLRPAITEYGDDLGVVGDTTWTAEIRNYLNGHP
                     ETNVVIWSWCGGVSENTEAGINAYLTAWSDLETDYPDVDFIYMTGHLDGTGPAGNLYA
                     RNNQIRAYCVEHGKTLFDFADIESYDPDGAYYPDETDACNWCADWCATHSCESCSCAH
                     SHCFNCYRKGQAFWWLLARLAGWSG"
     gene            complement(12262..12552)
                     /locus_tag="PKM94_10605"
     CDS             complement(12262..12552)
                     /locus_tag="PKM94_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67123.1"
                     /translation="MKRRLILGAAAALLLASAAPSCGDAPSVVQGAVVSYDPSSGIVV
                     LSDERAAGRTVALDLTGAEKGSDPAPGDTVRIAYRATDLRALRVMNVSRRFR"
     gene            complement(12549..13580)
                     /locus_tag="PKM94_10610"
     CDS             complement(12549..13580)
                     /locus_tag="PKM94_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD67124.1"
                     /translation="MDMLTIYLPIAGTEMNALLLLLIGATVGVCGGFFGIGGAWIVTP
                     ALNIFGFPMPYAIGTDLAHMGGKSIVATIRHSKFGNVDMRLAVAMMVGTMAGIELGAK
                     LVMALERHGLAESMIRTIYIVFLFLIGSYVLYDYRRHARRQAASPGDRAAPVRATLAQ
                     RLQRINLPPMVTFTTSRIRCSLWLPVLVGLVTGAVASVLGVGGGFIRMPALIYLVGCP
                     TLVAVGTDLFEVMVTGAYGGFTYGLKGRVDLLAAMWMLAGAAVGAQIGAVAVKYVRGY
                     SIRLLFAVTIFIAWASVLLKQVRLQLLSSVLILAAGIAISGVIVALLVQGMLRERTEA
                     RRAAGGKTP"
     gene            complement(13601..13855)
                     /locus_tag="PKM94_10615"
     CDS             complement(13601..13855)
                     /locus_tag="PKM94_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67125.1"
                     /translation="MSLAADRLWAEGTAEAARVYNVADSRGMEAGPSKWIADVYNTSS
                     WQYAVLVVAVMALMGLLLGYGTDRLLRLLRLNLGPLDHHE"
     gene            complement(13933..>14496)
                     /locus_tag="PKM94_10620"
     CDS             complement(13933..>14496)
                     /locus_tag="PKM94_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67126.1"
                     /translation="WVGIFLLGSLVGQMLARMRTGGMTADELAGRLRRAGLWLMALGL
                     VLNGAYKVFQLAGGALDPRVFDAVYPSRTTSLLPIYLAVLLWIFSFYVTVIDGRGRYG
                     RAAWALSVFGRTSLFTYVTQFVFAHSVPGLLGVRWRIGAEGFVLLLAFALPASWALSY
                     AYGRWRGWIAPHDYALLRGTTAPRGAV"
BASE COUNT         2561 a         5233 c         4376 g         2326 t
ORIGIN      
        1 gtcgccccga gcgcctccag cagcccggtc cgctcgatct cggcgaactt cggatcatcc
       61 gcctccacgc tgatgaagaa cccgccgccg gtcaccttct cgaactgcgg cgaactgaac
      121 aggggatggt gcagccgggg caggcggttg agggccagca tgccgaagag cgccgcgagc
      181 gccgaggaca ggatcgtgat cgcaaagagc ggcggcacga acgcctggta gctgaaatgg
      241 ggcttgcccg agatcaccat cggatagtcg tacacgttga tccagtagag gaaagccagg
      301 aacaccagca gcccggtcgt accgcacagg ccgacgatga atccgaccgg cgaacgcccc
      361 agccccatcg cccggtccat gccgtgaatc ggaaacggcg agtgcacgtc ccaccggcgg
      421 aaacccgcgt cccgcagctt ctccgccgcg cggagcaggt cgcccgtgct ccggaactcg
      481 gccagcacgc cgtacgcttt cctggcgcgc tcagccatgg ctgcgctccc cgtggtagtg
      541 cggatcggcc tgcggcagca ccgcctttac ctccgccatc gccaccatcg gcaaaaaccg
      601 caggaacaac aggaagagcg tgaagaacag gccgaacgac ccgatcaaca gccccacgtc
      661 gtgccaggtc gggctgtagt agtcccagct cgacggcagg aagtcgcgcg agagcgacat
      721 gacgatcacg aaacgctcga accacatgcc tatattaatg aggatcgacg cgacgaacat
      781 caccgggatc gccgtccgca gcctcttgaa ccaaaagagc tgcggcgtaa tgacgttgca
      841 ggtcaccatg atccagtagg cccaggcgta ggggccgaag gcccggttca cgaacgtgaa
      901 tttctcgtaa atgttgccgc tgtaccaggc gatgaaaaac tccatgccgt aggcgtaccc
      961 caccatcgac ccggtcacca gcatgatgat gttcatgcgc tcgagcactt tcagcgagat
     1021 gatgtgctcg aggccgtaga cgcgccgcgc gatcagcgac agcgtcatca ccatcgcaaa
     1081 ccccgagaag atggcgcccg ccacgaagta gggcgggaat atcgtcgtgt gccaccccgg
     1141 caccagcgag gtcgcaaaat cggtcgacac gatcgagtgc accgacagca ccagcggcgt
     1201 cgacaccccc gccagcacca gataggccag ctcatagtgg cgccactgcc ggttcccccc
     1261 ccgccagccg agcgagaaaa cgccgtacag aatcttccgt atcttcccct tcgcccggtc
     1321 ccggagcgtc gccagatcgg gcaccagccc gacataccag aacatcagcg agcacaggaa
     1381 gtacgtgccg accgcgaagg cgtcccacac caggccgctg cggaagttcg gccacatccc
     1441 catctggttc ggaatcggca tcgtccagta gatgaaccag acgcgcccga catggatggt
     1501 cggaaacatg agggcgcaga tcacggcgaa aatggtcatc gcctccgcga accggttgat
     1561 cgaagtccgc caccgctggc gcagcaggaa caggatcgcc gaaatgagcg tccccgcatg
     1621 gccgataccc acccagaaga cgaagttgac gatcgcgaat ccccatccca ccgggatatt
     1681 gagaccccag atccccgtcc ctttccagaa caggtagccg acgtgggcga agaacatacc
     1741 ggccagcgcg gccgagacca ggaacaacac ccaccaggcc ttcggggtcg cgcgctgctc
     1801 ggggatccgg ctcacggcct cggtgatttc ctcgtaagta ggattgccga cgatgaaacg
     1861 gtcctcgtcg atcaccaggg cgttcccttt ggcgtccttc gtgctcacgc cggcatctcc
     1921 ggattaaggt tgcggatgcg cgcgaggtag gtcgtgcgcg gctgagtgtt gtactcgccg
     1981 agcagcgcgt aatcgcggtt gctcttcttg acccggacga cgttcgactc cgggttgttg
     2041 atgtcgccga acacgatggc ctcggccgga cacgcctgct ggcaggcgct gaccacctcg
     2101 ccgtcgcgca gcgtccggtt ctccagcctg gcctgccgct tgccccggct gatccgctgg
     2161 atgcagtacg tgcatttttc catgaccccg cgcgagcgca ccgtcacctc ggggttctgc
     2221 tgcatccgga tcgtctccgg gtagttggcc gtgtagtggt agaagttgaa gcggcgcacc
     2281 ttgtaggggc agttgttgga gcagtagcgg gtgccgatgc accggttgta caccatctgg
     2341 ttgagtccct cggcgctgtg tacggtggcc gccaccgggc agacctgctc gcagggggcg
     2401 ttctcgcagt gctggcaggg catcggttgg tgcaccaccc ggggatcctc gaggtccccc
     2461 tcgaaatacc ggtcgacccg gatccagtgc atctcccgcc cgcggcggac ctggtctttc
     2521 ccgaccaccg ggatgttgtt ctccgcctgg caggcgaccg tgcaggcgtt gcagccggtg
     2581 caggcgctga gatcgatcgc catcccccac tggtggccgt ccttgtacga cggttcctgc
     2641 caggacgagg ggtactcgcg caccagttcc cgttcctgcg ccttggggct ctcggccatg
     2701 aacgccggga ccaccgccgc ccgggcggcg aaatcgggat cccggcggta ctcgtcgagg
     2761 gtcgcctcga gcacaatcgg ccgcccttcc atggatccgt gatcctgtgt tgtggccagc
     2821 tcgtacgacc ggccggtggg ggtgagcgag acgccggggg cggcgtgcat cgcggccgcg
     2881 gtccgcagac ggtaggtgtc gaacccgacg ccgctggcga cccggccggc cgcctcgcgg
     2941 ccgtacccga gcgccagccc gacgcagtag tcggcctgtc cgggcacgat ccaaaccggg
     3001 agcgtcagct cccgcccgtt caccgccacc ctgaccaaat cttcgttctt gacccccagt
     3061 tcctcggccg tcttcgggct cagggtcgcg acgttatccc aggcaagttt ggtgatcgga
     3121 tcggggagtt cctggagcca gccgttgttg gcctggcggc cgtcgccggt ggtgtaacag
     3181 ggatgaaaga tcccttcgag gccgcgggcg tcggccgaag cgacctccca ctggtgcgcc
     3241 ttgatcgcgg ccgcgatttc acccggccgc accgtggccg cgaccgccgc cgccgtctgc
     3301 ccgggcagga tgccgtcgtg gagagtctgc cgccagccgt tttcaaaatc gccggcgatc
     3361 agcccgcgcc aggtctcgcg cacgaggtcg tacccgcggg cgtcatctcc cccggccaga
     3421 agtgcgagca cttcgctgtc gctctttcct ccgtacagcg gctcgatgag cggctggacg
     3481 agactcagcg tgccgtcgac cgcccgcgcg tccccccagg cctccaggta gtgcgcgcgc
     3541 ggcagatgcc acagggccgc ctgcgaagtt tcgtcgcggt gcgtgctcag atgcacggtg
     3601 gtcggcacct tggcgagggc tgcgcggaat tcgaggtcgg ccggggcgtt gtaggccggg
     3661 ttgccgccga gcatcacgag cgcctggatc tcgccggaag ccatcccctt cacgagctcc
     3721 gccagcccgg ggctgtcgga gcgggcaacg tccggattgt ccacatacgt aatggtgcgg
     3781 cccgtgttgc cgagggcctc gttgagcgct gccgccagcg cgtgcaccgc cgaaggctgc
     3841 ctgcgcccga ccaccaccag gctctccccg cgcgcccgca aaagatcacc ggccaacggg
     3901 gccagccact gcgccgccgc accggagagc tgcgggattt tcgccccgcc gagcatctcg
     3961 aggggcagtc cctgccgggc cagttcggcc gccagggcga gcgcaaacga gccgacctcg
     4021 cgcgcacgca ggcgcagccg gtggtcggcg ttggcgcccg tgaccgacag caacccctcc
     4081 accgcataca gacggttcat ccggtctttc tcggaggtcg tccgccgccc ggcggcaaac
     4141 ccgtgcgtcg ccgccaccat ctcatcttcg gtcagcagga aatcgctgtc gagggagagg
     4201 atcaccttcg cccggtcgta gtggaagacg ggctggaggt cgcgcccgaa ggcgatccgg
     4261 agcgcctcct gccgccgctc gtcggccacc ggcgcccagg cgatccagcg ggcgttgggg
     4321 aaccgctgct gaaacgcccg tcgcaaccgc gcgatcgtcg gcgatgacga cggctccgcg
     4381 agcaccgcca gcccgcgccc gccgccggcc tggaaagcga ggaactgctc gcgccagaac
     4441 gtcacgaaag cgccccagtc ggtgttgacc ccctcgcgca caaaggcggc ggagcgatcg
     4501 ggatcgtaga agtcgagaat ggacgcctgg atcgcggcgt tggccgcccc gcgcgtcgat
     4561 ggatgcagcg ggttgccctc gatcttggtc ggccgcccct cgtgcgactc cacgaggatc
     4621 ccgtaggccg aggtccccca cggcatcgtg gtcgcgtagg tgaccgggat gccggggaca
     4681 aggtcctcgg ggggctggac gtagggaacg atcttctcct cgggccggcg gcagccggag
     4741 aggccggcga gcgccagcga ggctcccatg agggtgagga aactccgccg gctccaggca
     4801 ttggcccact cggaggctcc ctggggaaac tcgcggtgca gccactcctc gaactccggc
     4861 gtccccgtca actggtcgag actgcgccag tactgcctgc ccgtcaggga ccggtcgtcc
     4921 ttcagcgatg acatgtccag caatcctccg acggccgaat gtggtgttcc tgcatgaagc
     4981 gctccgccca gtcggcgtgg tcgggcgcgg gcgcccaggt catattcgta atctgatcga
     5041 gcgggcgaag gtgcggcgcc gggttccggt ggcagctcag acaccagctc atgctgagcg
     5101 gctggacctg ctcgacaact tccatctgcg ccacgttgcc gtggcagctg aaacacccga
     5161 cgccgctctg cacgtgccgg gcgtggtcga aataggcgta gtcgggcatc ttgtggatcc
     5221 gcacccactc gatcgggcgg ccggtcgcga aactctcccg caccggcagc agcttctggc
     5281 tctccggcag aatcagcttg tggcagttca tgcacgtctg cgtcggcgga accgtcgcgt
     5341 atcgcgactg ctccaccgcc gtgtggcagt agcggcagtc gattccgaga tccccggcgt
     5401 gcagcttgtg gctgtaggcc acgggctgaa ccggccggta cccgacgtcg gtgtagcggg
     5461 gggacccgta gtaccagacg ccgaggaccg cggccgcgcc gatcacgatc gccgccgccg
     5521 ccagcagcag gggcaaacga tttgtccaga ctggaaagat ctgagccaaa accaattatc
     5581 tctgcgccga tcgagggatc ctcgcgcgcg cgggcgcggt tcggggcgcg caacgaatcc
     5641 gcccggttcc atcccaatca tgtacagccg acagtccgca acgatacgcc tccgtcggcc
     5701 cgctgtcaac acaaaaagac gctatcgaag aacaaaatag tccatttatc gcaaccgccg
     5761 cccccggccg gccggagtct ctccggcgcg gcggccgcgc cgactttgta ccgacgaacc
     5821 tccctggccc aacgaacctt agccaacgca aatgcggacc gatcggtccg catgcgggaa
     5881 cctctgccgc acccgcgcct acagccagcg ctgcagcacg ccgtccgcca tcatcgcccc
     5941 gaaaaggcta aacaggtaca cgatcgagta gccgaacagc gaccggtatc cggcgaccga
     6001 ctgctcccgg cgcacgcggg ccgcccggcg gatgaactgc ccgcccagaa gcgcggccac
     6061 ggcgaagtag aaccacccgg ccgtgaacgc gatcagcacc aggctcgccc ccaccactgc
     6121 cagcgtgtac gcgaaaatcc ggtcgaccgt cgcccgatcc cccttcacca ggggcagcat
     6181 cggcagatcg accttctcgt agtcgtcctt gcagtagaga gccagcgccc agaagtgcgg
     6241 cggcgtccag aggaagacga tcgcgaacat gatccacggc gcaaccgcca tcgctcccgt
     6301 cgccgccgtc cacgccccca ccggcgccat ggccccggcg attccgccga tcacgatgtt
     6361 ctgcggcgtc gtcggcttca ggtacagcgt gtagaagagg ctgtagaaca ggatggtcgc
     6421 cagcgccagc gcggccgtga gcaggttgaa gaagagtgcg aagacggcca cgccgagcac
     6481 gccgatcgcc accgagaaaa ccagcgcccc ggccgggctg atctgcttgc gcggcagcgg
     6541 ccgccgcccg ctcgtccgct tcatctgcgc atcgatgttc cgctcgaaat actggttgag
     6601 ggcgttggcc gagccgcccg ccaggtacag gccgagcagc acgagcagga accgccccgg
     6661 ccggtcgagc agactcccct cgatcacgag cgaagccgtc cccgtgaaca ccaccagcag
     6721 catgatcgac ggtttggtca attgaaagta atgtgcgagc gtcgctttca tcggcctgtt
     6781 tctctccgcc ggcgggacct tccccgcggc tcgcgctctg caaccgcgca cccccgccga
     6841 aagttccgcg cttcgcggcc gggaacgccg ttaaatcgag caaacttgtc caccttatta
     6901 cggtattccg actccgttgt caatatccgc ggccgcgaaa cttgactttt ttctccccgg
     6961 tcgctatttt ggccaccgtg tacgcgccgc aaccggtata tcgggagtgc accacgcgcg
     7021 ccgcctgtct tttccggcgg gtccggttcg cgctgctccc cgccgcggtc gcgctcgtcc
     7081 tcggcgcccc tcgcaccgcc gccgcggcga taatcgaggg aactatctgc gactcggcca
     7141 cccacgagcc tatcccgcgg gtcacaatcc gcgtcgagca tgaggaccgg gtgatgttct
     7201 ccaacgagga cggctactac cgtctccggc tcgaccccgg aacccatcgc ctgcgcttca
     7261 cccacatcgc ctatttcccg gcggcctgcg acgtcgccct gagcgactcg acctcgtccc
     7321 gggacatcct cctcgcctcc tgcctgatcg acgtccccgg cacgcgtgtc tactcccggg
     7381 cctacgaccc cgcgcaggag attatcgtcg aggcgatccg gcacaaagag gagctgctcg
     7441 cccggatcag ggactaccga ttcgagggct acacgaaact cgcggtgcgc cggctcactg
     7501 acgacgggcc tgccgagtat ttcgcgatca tcgaaagcca gctcgagggt tggttccggc
     7561 aacccgaccg ctacaaggag atcatcaccg cccggcgcca gtccgcgaac atccccgccg
     7621 aagtgaatat catcacgatc ggcgagatct tcaacttcaa cgccaaccgc atcgacctcg
     7681 ggcagccggt cgtttccccc accgcctccg acgctctcga ctactacaat tattacctgc
     7741 tggatacggt gctggtcgac ggcgcgccgg cgtttctcct ggaggtcgaa ccccgcaccg
     7801 atgcccacgc tctcttcgcc ggccggattc tgatcgtcga ttcggtctac gccgtcgccg
     7861 gcgtcgacct cggcctgaac aagggcttcg actcgccgta cctgaaggat cttcgcttca
     7921 cccagcgcat gtcccgcttc gacaatgaca tctggatgcc ggttgagatc cggttcgacg
     7981 gcgatttcca gtcgccgctc ccgctcattc ccgacctccg cttcgcctac accggcctgc
     8041 tcaaccgcct cgtgatcaac cagggcatcc ccgaggagcg cttcgaccgc tacgttctcg
     8101 aggtggcgcc ggaggccgac gacctcgact cggccgcctg ggagcagggc gccctcctgc
     8161 ccctgaccga gagtgagcag acgggctacc ggtacctcga ctcgctgaag caccgtcccc
     8221 tcacgccgcg gcgggccctg gggtacggcg tcatgctcgc ggcggcgggc accatggcgc
     8281 agtccgccca caagttcttc cacttcaacc gggtcgaggg accctacctc ggcgcgggcg
     8341 ccaatctgac caaactcgtt ccccgctccg aattgtccct caaaaccggc cgcgccttcg
     8401 acgccgactg ctggcagcac cattaccggc tgcaggtgct gctccacgag ccgacgcagt
     8461 tgaaagcggc catcggctac cgcgatgaaa tcgtcgcccg gcccaacatc tacggcgacc
     8521 aggccctcaa ccccactttc gccaacctgt tcttcaagta cgaccccatc gactattacc
     8581 ttgagaaagg ttttgacctc gggctgtcgc tgcgcatcct cccgcacacc tccgccacac
     8641 tcggctaccg cgacttccgg cagtcgacag tcggcaacac caccgaatac agcctgaccg
     8701 gcatcgaccg cgcttaccgc tccaacccgg tgatcgccga cggccgtctg cgcgccctct
     8761 ccgccggcct cgcttacgac tcccggccgc tggttcgttt caagggccgg gagcaacagg
     8821 tcatggcgta cccctacacg tcggtgcggc tcgagttcga agccgccgat ccggatctga
     8881 tcgccaccga ttttcacttc cgccggtacg cgctctcgct ggtgcgggtg cagtcgctgg
     8941 ccggctgggg attcacgtcg ctccgcgcgt atgccggcgg ctcggactac gccctgccgc
     9001 cccagcgctt tttcacggtc tcgctggaga acccgtacga gccgttcctc gagttcaaaa
     9061 cgctcagcga tttcaacgtc accggcgacc gcgtcgccgc attctacctc gagcatgatt
     9121 tcggaggcac gctcttccgg cgcctcggcc tgcccctgct ggaggagttg ccgttcggtc
     9181 tggcggttta cggcggatcg ttctggaccg atatccgcgc cgccacccgc gccagcttcc
     9241 ccgctgcctt cgccggtgcg ccggacccgc ccaaaagcgc gccggactgg tattccgaaa
     9301 tcggcttcgg catttcccgc ctgcccctct tggtggagct gctcttcacc tggcgggtct
     9361 cggcccacga gagcgaccgc ttcagcttca aattcaacat gagcttctga ttcgggccaa
     9421 aagaaaccgg gcggcccgcc cggccgcccg gtcaaactcc tgtccctgcc actctgttaa
     9481 agggtgaaag cgatgaagaa gtaacggtcc tggcgctgga cgcgcagcag gacgtcgtcc
     9541 ccctttccaa cccccgacaa caggctccgg agctcatcga ccgatcgcac ctgccgccga
     9601 ttgaccgccc gaaccacgtc cccttcgcgc agccccgcct ggtacgcgga gctctcggaa
     9661 tccacggcga tcacgacggc gccgccggcc ccgccttcca ctccgtactc gcgcgccaga
     9721 tctcgggtca actcggccac ctggaaaccg agggtctcgt ccagcgatga tccgggcccc
     9781 ccggccgcgg ccgcctcggc cgtcatctcc accagcttga cccacacctg gtttttcgcg
     9841 ccctcatgga acacagtcag gctggcccgg gtccccgggc gaatttcggc gatccggttg
     9901 cgcaactccg tggaattctg gaccgtctga ccgtcgatcg cgaggatcac atccccttcc
     9961 ttcagacccg catcctcggc cggacttccc ttcaccacct cctgcacgag cgccccctgg
    10021 gtgctctcca ggccgagggt gcggctcatc gccggcgaga tgtccgagat cgacaccccc
    10081 agccagccgc ggaccacgtg cccttcctga atgagcgact ccatcacgta cttcaccatg
    10141 ttcacgggca ccgcgaaacc gatcccctgg tacccgccgc tctgagaggc gatcgccgtg
    10201 ttgatcccaa tcaactcccc gtcgagattc accagcggcc cgcccgaatt gcccggattg
    10261 atggctgcgt cggtctggat gaagtcctcg tagtcggcca gcccgacgtt ggaccgccct
    10321 ttggcgctga caatcccctg cgttaccgtc tgcgccagat tgggcgagag cggactgccg
    10381 accgcgacca cccactcgcc gaccctgata tcgtcgctgt tgccgaggcg gatggctcga
    10441 aggccgttgg cgtcgatgcg cagcacggca atgtcggtgc ggggatcggt cccgatcact
    10501 ttcgccgtct gccgctgccc gtccgatgtc tgcacataga tggtgtcggc gtcctggacg
    10561 acatggttat tagtgacaat gtagccgtcc tcgctgatga tcactccgga gccgagcccg
    10621 gtctgccggt actcctgttc cggcgcctgc cgccccggcg tgccgaagaa ctgatcgaaa
    10681 aagcctccga aggggctgtt gtagaagggg ttgcgcatcc gaaggacgcg ctcagagaag
    10741 acggtcacga ccgatggccg cacctcgtcg gcgatctcga caaacgcgtt cccgaggtcg
    10801 cgcagcgatt gtatcggccg gtcggccggg ccggccgcct gccgctggtt ctgcgcttgg
    10861 acggaattgg ggtagtcgaa gtactccgtg atgattccgc cggcaacaag cagcccgatg
    10921 acaaccagca tcggcgtcaa atacttgccc cgaatcataa ccttggcact cctttacact
    10981 ggtgaggccg ccttcccgcc tcacggttct ttccaatata gtacaccctt ccgcccggcc
    11041 ggtttcctgc cgattcgcgc tccccgcccg accgcccagc gtccggtttt tagttgccaa
    11101 cggcccccgg ggaatgtaga tactctggtg caatctttca ttctacacaa gaggccgata
    11161 tgagagccat ccccgccccc gcccttcggg ccgccttcct catggcggcg gtcgcactcg
    11221 ccgtggaatg ctccgatgac tataaaagca cggataaccc ggagcccgga agcccggtcc
    11281 tggccgccat cggcccccgg agcacgacga cagacgtccc gctcgccttc accgtcaccg
    11341 ccaccgaccc ggacggccaa atccccgcac tgtcggccgt cgacctccct gagggcgcat
    11401 cgttcaccga ccacgccacc ggcgccggcg acttcgcctg gacccccacg ctcgcgcagg
    11461 ccggaaccca ccaggtgacg ttcatcgctt ccgacggcgc cctcaccgac caggagatcg
    11521 tgacgatcac ggtcagcgaa ccgggcgagc agacagcgat catcgccgac caccgcgcgc
    11581 ccgagcgggc ggcgcagatt cccggcgcga tcctcgaccg aatccgcgac agcctcacca
    11641 tttacttcgg ccacacctcg cacggctccc agttgctcac cggcatggat ctgctggcag
    11701 ggatgagttc cgcctacctc cggccggcca tcaccgagta cggcgatgac ctcggggtgg
    11761 tcggcgacac cacctggacc gccgaaatca gaaactacct caacggtcac ccggaaacga
    11821 atgtggtgat ctggtcgtgg tgcggcggcg tgtccgaaaa tacggaagcc ggcatcaacg
    11881 cctacctgac cgcctggtcc gacctcgaaa ccgattaccc cgacgtggac ttcatctaca
    11941 tgaccggcca tctcgacggc accggccccg ccgggaacct ctatgcgcgc aataaccaga
    12001 tccgggccta ctgcgtcgag cacggaaaga ccctcttcga ctttgccgac atcgaaagct
    12061 acgatcccga tggcgcttac tacccggatg aaaccgacgc ctgcaactgg tgcgcggact
    12121 ggtgcgccac gcattcgtgc gagagctgca gttgcgccca ctcccactgc ttcaattgtt
    12181 accgcaaggg ccaggcgttt tggtggctgt tggcccgcct cgccggctgg tcgggatgag
    12241 cgcggcccct gccggctgcg cctacctgaa ccgacggctg acattcatga cgcgcagggc
    12301 gcggagatcg gtcgcgcggt aggcgatccg tacggtgtcc cccggcgccg gatcggagcc
    12361 tttctcggcc ccggtcaggt cgagcgccac cgtcctcccc gccgcccgtt catcggacag
    12421 gaccacgatc ccggaagagg gatcatagct gaccacagcc ccctgcacga cggacggggc
    12481 gtcgccgcac gatggggcgg cggacgcgag cagcagagcc gcggctgccc ccaggatcag
    12541 gcgcctcttc atggcgtctt cccccccgcc gcccgccggg cctccgtccg ctcccgcagc
    12601 atcccctgca cgagcaacgc cacgatcacg ccgctgatcg caattcccgc cgcgaggatc
    12661 agaaccgacg acagcagttg caggcggact tgtttcagga ggaccgaggc ccaggcaatg
    12721 aagatggtga cggcgaacaa cagccggatc gaatagcccc gcacatactt caccgccacc
    12781 gcgccgatct gcgcgcccac cgccgccccc gccagcatcc acatcgccgc cagcagatcg
    12841 acccgcccct tgagaccgta cgtgaacccg ccgtaggccc cggtgaccat cacctcaaac
    12901 agatccgtcc ccaccgccac cagcgtcgga cagccgacca ggtagatgag cgcgggcatg
    12961 cggatgaatc cgccgcccac tcccagcacg ctggcgactg cgccggtgac gagaccgacg
    13021 aggaccggca gccagagcga gcaccggatg cgcgaggtcg tgaaagtgac catcggcgga
    13081 aggttgatcc gctggagccg ctgcgccagc gtcgcccgca ccggcgccgc ccgatcaccc
    13141 ggcgatgccg cctgccgccg agcgtgtctc cggtaatcgt acagcacgta gctcccgatg
    13201 aggaatagaa agacgatgta gatcgtccga atcatcgatt ccgccaggcc gtgccgctcc
    13261 agagccatca ccagtttcgc tcccagctcg atgcccgcca tcgtgcccac catcatcgcc
    13321 accgccagcc gcatgtcgac attgccgaat ttgctgtggc ggatcgtggc gacgatcgat
    13381 ttccccccca tgtgcgcaag gtcggtgccg atcgcgtagg gcatggggaa accgaagatg
    13441 ttcagagcgg gcgtgacaat ccacgcgccg ccgatgccga aaaatccccc gcacaccccc
    13501 accgttgccc cgatgagcag caggagcaga gcgttcattt ccgtcccggc gatcggcagg
    13561 tagatggtca gcatatccat gcccgtgttc ccccccgccg ctactcgtga tggtcgagag
    13621 gaccgaggtt gagacgcagc agccggagga ggcggtcggt gccgtagccc aggaggaggc
    13681 ccatgagcgc catgaccgcc acgaccagga cggcgtactg ccaggagctg gtgttgtata
    13741 cgtcggcgat ccacttggac gggcccgcct ccattccgcg cgagtcggcc acgttgtaaa
    13801 ctctcgccgc ctcggcggtc ccctccgccc agagccgatc ggctgccagg gacaccgtgg
    13861 cccacgccgc gagcgcgcca ggccaggctt ttccggcacc cgcccggttc cgccgtgagc
    13921 cggccgcacg gttcatacgg cgcctcgcgg tgccgtggtt ccgcgaagca gcgcatagtc
    13981 atggggcgca atccacccgc gccaccgccc gtatgcatac gagagcgccc acgaagcggg
    14041 cagcgcgaac gctaacagca gcacgaatcc ctcggcgccg atccgccacc gcacccccag
    14101 caacccgggc acggaatggg cgaagacaaa ctgcgtcaca taggtgaaca gcgaggttct
    14161 cccgaacacg gacagcgccc aggccgcccg gccataacgc ccccggccgt cgatcacggt
    14221 gacgtagaaa gagaatatcc acaggagcac ggccaggtag atcggcagca gggaggtggt
    14281 gcgcgacgga tagacggcgt caaacacgcg cgggtccagt gcgcccccgg ccagctggaa
    14341 aaccttgtaa gcgccgttca agaccagccc gagcgccatc agccacagcc ccgcccgtcg
    14401 cagcctcccg gccaactcgt cggccgtcat cccccccgtc cgcattcggg caagcatctg
    14461 ccccaccagc gacccaagga gaaagatccc gaccca
//
LOCUS       DAOPOE010000083        14187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5028_length_14187_cov_5.679309, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000083 DAOPOE010000000
VERSION     DAOPOE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_14187_cov_5.679309"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1107
                     /locus_tag="PKM94_10625"
     CDS             232..1107
                     /locus_tag="PKM94_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67127.1"
                     /translation="MIALRRPPEGRTRLAWHELEQIQTNEWVLKLRPPYYGAVFADRE
                     PDLAAKSPAGFHIARTGAVAWAVFRGLTPRDLARINDFAGAYRRFVLLGDNPNLKGQF
                     SDELDGCLALDYNFAESRSRSYTEIGELVYHAKYRRSVAAAEELGRRLAEAAGRVPTM
                     RCVSRPCLSYVPPGPHKRFDLPRILARLLVDYGWCTMPLREFEPLVHPELSLAKPDFK
                     RLTIDEKIRAWTRLLARGVRLSGNVAGAAVYVIDDLYQSGATLWSYARYLKAAGAAAV
                     FGLVCEKSRGDRDNR"
     gene            1104..2030
                     /locus_tag="PKM94_10630"
     CDS             1104..2030
                     /locus_tag="PKM94_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD67128.1"
                     /translation="MNRETALLRLLAAPGLGARSVARLLLRLAAEQLPLEDFVALPAD
                     HLVREYALAPASADALASAGEEARELAEHLPAHRIRMVTIVDDGYPELLARRLGRTAP
                     PVLFTRGDLGLLRVPMVACAGARECSAPGLAFARALAAALVRHGANIVSGYAPGIDTA
                     AHGGALAAGGVTTAVLASGILHFTPRPGLASLGDETNTLVVSEFFPTMPWRSHSAMQR
                     NRTICAMAHAMVIVESGVSGGTFAAGRAALEVGCPLCVVDWQDPPPSAEGNRLLIRRG
                     GVPVADLSGALRELTRRLHSEGPGPVTPELFG"
     gene            2117..3670
                     /locus_tag="PKM94_10635"
     CDS             2117..3670
                     /locus_tag="PKM94_10635"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOD67129.1"
                     /translation="MPRRLRRLPMKEDLMSAQAVYDELTRKLREMALLSSAGSVLHWD
                     QQTYMPPEAAQFRADQLGTISGLVHRMFTDPKIGDMIGTVENSDLAKGDTPEAANIRE
                     LRHDYDKMTKLPNSLVEELTRTTAMAQQVWAEARRKSDFAMFLPHLEKVLALTRRKAD
                     AYGWKDEPYNALIDDYEPGANVREVGEVFESLRKELVVLNEKIRNAPRKPDHTIVERE
                     YEVERQKVFGESVATAMGFNFRAGRLDITTHPFCTGIGYGDTRILTRYNPNRLNDALF
                     GIMHEAGHGLYEAGLLKSEYYGTPMAESVSLGIHESQSRMWENQVGRSKSFWKYFFPQ
                     AQRLFRKSLGDVTLDRFYGAINYVTPSYIRVEADEATYNLHILLRFEIERALLTGDMK
                     PADAASEWNARFEKYFGLKVDKDAHGVLQDVHWSAGLVGYFPTYTLGNLYSAMFFAQA
                     RKEMPDLDLQFERGELLPLREWLREKIHRHGRRYRANELCRKITGKSLDYKPLVVYMT
                     EKYSEIYGF"
     gene            complement(3744..3959)
                     /locus_tag="PKM94_10640"
     CDS             complement(3744..3959)
                     /locus_tag="PKM94_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67130.1"
                     /translation="MSDDGRAVESAGPAKPAVGKARWNLLRFPRSWKEVRALGWRMIV
                     AFIFFYLIRDTLLYIVFPYLAYKGIFD"
     gene            4107..5462
                     /gene="trkA"
                     /locus_tag="PKM94_10645"
     CDS             4107..5462
                     /gene="trkA"
                     /locus_tag="PKM94_10645"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOD67131.1"
                     /translation="MRIVIIGGGVVGFSLAEHLLRDGHTLSLVELDPHLCRMIEEKLD
                     LQVVSGSGASPVTLREAGIEGADMMLAVTPSNEVNIVACSIAALHEVPSRIARLRGQE
                     FSARGGLVDLTRMGITALIQPEQAVADHILQFMETPHAVESANFEGGRILLRGYAVKD
                     TMPLAGKTLREIRAHIDPAVVLFAAIVRAGKGMIPDGNTRIEPGDIVYALFPRESLGV
                     FLDLVAIERKESRKVIVTGDAFFTLELAEALERTPHKVTLVDPSREHAEKAAAMFNRT
                     EVIHGDCTETDLLRELNVAAASFFIAASDEADYNVFSSLLAKAEGAREVIAVSTESRH
                     DRLFNSIGIDHVVNPRLITAREILEIISRGHIGAVVKLSDVDIEAVRFTVEEPSEIAG
                     QQVKRIARRLKKGSIIGVIIRGSSMILPGGDTVIEAGDHVIMISSHKNLKMLDRLFSG
                     R"
     gene            5485..7086
                     /locus_tag="PKM94_10650"
     CDS             5485..7086
                     /locus_tag="PKM94_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOD67132.1"
                     /translation="MNVAAVLYALGKLMQVMGGFLLAPLAIALWDGRVQGLPRLFAAA
                     DASGLLVAAVFGLAVGTLLAALFRRGRGRQGLREGYAIVTIGWLTLTFVAALPLFLYF
                     WRVGMPDGSVSGLRAFTDAYFEIMSGFSTTGATILSNIEAVPRSLLFLRSLTHWLGGM
                     GIITLAIVIFPAMGVSAYSMYRGEVPGPSHDKLRPRLAQTAQVLWGVYVLLTALQTVL
                     LIFGGMTWFDAVNHSFATMATGGFSTRNASVAAYNSPYIEWVIIVFMYLAGVNFLLHF
                     KALRGDLRSLTHNSEFIFYSAVIALAALAATIVLYLQGLEPMAEAARSFRAHPPTETA
                     FLERYLTEQSRLEGLADCFRAALFQVVSVTTTTGFSTADFDMWPDFVRFLLMVLMFFG
                     SMAGSTGGGIKMIRVMVVAKAAANVVRKLTQPRLVAPVKVGGQPLDNDKIISIAALFV
                     FFTGLFVLTSMLLMLFIPDMITAASASIATLGNVGPGLAGVGAVENYGWIPAPAKWLL
                     VLSMLLGRLEIFTVLIVLRPAIWRK"
     gene            7195..8388
                     /locus_tag="PKM94_10655"
     CDS             7195..8388
                     /locus_tag="PKM94_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD67133.1"
                     /translation="MRSFFPLIFATAVILVFGSVEILLLRLFNPSWWRVRWIRRTGLL
                     LPLVGTLCAGLWGLGEYYAASWLAAPGAAATALAFVLLVALMLSLPVSGIVHFLRWTF
                     DKLRSRRATAPDLPDHNRRLVFKAVAAAIPAATLGAGLGGVARAFTGVAVAPREFPLP
                     GLPAPLDGLRIFHLSDLHLRHYVTLDDLEEVLRKAEPLRPDLVLVTGDVADDLALLFD
                     ALRMIDGFRAPLGAFASLGNHEYFRGIEEVRAIFDRSPVPLLVDRGVPLAVGGRRLYV
                     AGIDDPRRMGAKEHEFYRRTIDAALQDAASDDTVVLMSHRPDAFDYAAQTGVSLTLAG
                     HTHGGQIGLMGRSAFEPLWPDRYLWGEYALSESRLYTTAGVGHWFPFRLGCPQEAPVL
                     VLRRG"
     gene            8743..9258
                     /locus_tag="PKM94_10660"
     CDS             8743..9258
                     /locus_tag="PKM94_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOD67134.1"
                     /translation="MAKKSVKKAPAAKKPLKKKAAPKKKKAAKKPAKKAAPKKKAAPK
                     RKTAKKATKKAVRKTAKKATPKKKTAKKTAKRATKKAAKKKASKRAPNPAFMKPLTPS
                     PELADVVGSKPMPRTEVTKKLWAYIKKNNLQDAKNKRMINADDKLRKVFGGRGQVSMF
                     DMTKHVSKHLK"
     gene            9414..11546
                     /locus_tag="PKM94_10665"
     CDS             9414..11546
                     /locus_tag="PKM94_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD67135.1"
                     /translation="MPAPLTASMLRLAVLIAAALAIAAAPSVPAAPPPTPVHTVIDTL
                     HGVALADPYRWLEGGDSAAIAGWTEAQWTYLREYVDTYPDRAKLHDRIAALMSMGSIS
                     TPEVRDSLYFFKRRDGSQNQPVLYVQRGLAGQPAVLLDPNTLSADGTAALDWSHLTDD
                     GSLLAYGVSASGSEQSVLYIMRTADRTRLPDTIPYTTSVSLAWLPDNSGFYYTRFPEP
                     GTVPPGDERYHRWVYLHRLGDDWRADSLIFGSELDKAAWPGVGLSPDGRRLLVAVYRG
                     WSDIDTYVRDRTDPASSFTRLYDDVEATVNVTPLDDCFLAFTNLNAPRFRLMRGQYEK
                     PGIENWVEIIPEREAILESAAPVGGRLVTRSLQNAYSVVEVFTMEGRLLRTLPTPSPG
                     TVYSVSGELDGRELFYYFSSFDTPSAIYRYDFAADTVALWAQLDAGVDLSNLVVKQVW
                     YKSHDGTPVSMFLVHREGIPLDGSNPAFLYGYGGFNVTMTPYFSRTLAVWYDQGGIYA
                     LPNIRGGGEYGEAWHDAGKLGNKQNSFDDFIAAAEYLIAEGYTSPARLCISGGSNGGL
                     LTGAVLVQRPDLFRAAIVAVPLLDMIRYHRFLAARIWVPEYGSSEDPEQFAYLLAYSP
                     YHHVTESADYPAVLLTAGLSDSRVDPMHARKMTARLQAADATPETPVLLRIEPKAGHG
                     QGKPISKQIEEQTDQWSFVFRALNVRMK"
     gene            complement(11713..12159)
                     /gene="msrB"
                     /locus_tag="PKM94_10670"
     CDS             complement(11713..12159)
                     /gene="msrB"
                     /locus_tag="PKM94_10670"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOD67136.1"
                     /translation="MGEKIQKTDEEWRAQLTPEQYRITRECGTEPPFSGKYYYHREDG
                     TYRCIGCGNALFTSGTKYESGSGWPSFWQPASPQAIEQKTDRSHGMARTEVVCGKCGA
                     HLGHVFPDGPRPTGLRYCINSVALDFTKDTAAADSADAAADSSKGD"
     gene            12473..12961
                     /locus_tag="PKM94_10675"
     CDS             12473..12961
                     /locus_tag="PKM94_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD67137.1"
                     /translation="MTNSENLLYMMRINQRALKALLDDITEEESMVRGRDSLLHIRWL
                     TGHLVYNVNHVLAMIGSLPVMLPSSYKQLFDRGSAITNDPKAYPSLAALRSELLDLHD
                     RLIDVLPGVSDDDLDEPLPKDAGFNAVRLNAASFMCMHTFYHCGQIAMIRRVLGRERS
                     FG"
     gene            13274..>14187
                     /locus_tag="PKM94_10680"
     CDS             13274..>14187
                     /locus_tag="PKM94_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67138.1"
                     /translation="MNRTQSNSQRGSALLIALVLTGMLSLIALMAIDNSQTEIDLSFN
                     KINSDRSFYIAEAGVQHAHTVINATPGWNAGLAGVAFAGGAYSVSVRDSLSEPALADS
                     VVLLSQGEVNGARSTIEATLAPEPFYPFRYGLFADSGVAIGNSVSTDSWNSDSASYAG
                     SAVNAYGSVGSNGPIVIENNPTISGDVYTAQAGAITITGTGRVLGDTSSQAPELDFSK
                     LISPTQFADAEAVNRAPLGMSGSFTYNNGSRDLRIGAGQTMVLESGTYVLDDILLEQS
                     AKLVLAPGAQVELYVTGNIDLRNKATVNS"
BASE COUNT         2395 a         5273 c         4123 g         2396 t
ORIGIN      
        1 gtggtcgagc acccggaatt ccctgtcgag caggtcatcg gcggctttgt tctcggtatg
       61 cgccatgtga ttctccggag tttgcccccg aatatagcgc ggccgccggg gcggggcaaa
      121 cggtttgcac gagggcccgg caaaatgtga ttgccccccg cctccccgcc cgctatcgtc
      181 agcccggacg cacgaccaag gcccgaccac gaacttgtcc cggggaggaa catgatcgca
      241 cttcggcgcc cgcctgaagg ccgcacgagg ctggcctggc acgaactcga gcagatacag
      301 accaacgagt gggtcctcaa gctccgcccc ccgtactacg gcgccgtgtt cgccgaccgc
      361 gagccggacc tggccgccaa atcccccgcc ggattccata tcgcccggac gggcgcggtc
      421 gcctgggcgg tgtttcgcgg gctcaccccg cgcgacctcg cccgcatcaa cgacttcgcc
      481 ggcgcctacc gccggttcgt cctccttggc gacaacccga acctcaaggg gcagttctcc
      541 gatgagctcg acggctgcct ggcccttgac tacaatttcg ccgaaagccg cagccgcagc
      601 tataccgaga tcggcgaact cgtctaccac gccaagtacc gccggtcggt ggccgcggcc
      661 gaggaactcg ggcggcggct ggccgaggcg gccggccgcg taccgaccat gaggtgcgtc
      721 tcccgcccct gcctctcgta cgtgccgccg ggaccgcaca agcggttcga tctcccccgg
      781 attctcgccc ggctgttggt cgattacggc tggtgcacca tgcctctgcg tgaattcgag
      841 ccgctcgtcc accccgagct gagcctggcc aagcccgatt tcaaacgtct gaccatcgac
      901 gagaaaatcc gcgcctggac ccggctcctt gcccgcggag tgcgcctctc gggcaatgtc
      961 gccggcgccg ccgtctatgt gatcgatgac ctctaccagt cgggcgcgac cctatggtca
     1021 tatgcccggt acctcaaggc ggccggggct gccgccgtct tcgggctggt ctgcgagaaa
     1081 tcgcgaggcg accgggataa ccgatgaacc gcgaaaccgc cctcctgcgc ttgctcgccg
     1141 cccccggcct cggggcgcgc tcggtcgctc gcctcctgct ccggctcgcg gcggaacagc
     1201 tcccgcttga ggatttcgtc gccctccccg ccgaccacct cgtgcgggag tatgcgcttg
     1261 cgccggcgtc ggccgacgcc ctcgcgagcg ccggggagga ggcgcgggaa ctggccgagc
     1321 atctcccggc ccaccggatc cggatggtga cgatcgtgga cgacggctac ccggagcttt
     1381 tggcccgccg cctcggccgc accgcgccgc ccgtgctttt cacccggggc gacctcggcc
     1441 tgctgcgggt gccgatggtc gcctgtgcgg gcgcccgcga gtgctccgcg cccggcctgg
     1501 cctttgcccg cgcgcttgcc gccgcgctcg tccgccacgg cgccaatatc gtcagcggct
     1561 acgccccggg catcgacacc gccgctcacg gcggcgccct cgccgccggt ggcgtcacca
     1621 ccgccgtcct ggcctcggga attctccatt tcaccccgcg gcccggcctg gcgtccctcg
     1681 gcgacgagac caacaccctg gtcgtgtcgg agtttttccc gaccatgccg tggcggagcc
     1741 acagcgcaat gcagcgcaac aggacaattt gcgcgatggc ccatgctatg gtgattgtgg
     1801 aatcgggagt ttcagggggg actttcgccg ccggccgggc cgcgctcgaa gtcggctgcc
     1861 ccctctgcgt cgtcgactgg caagatccgc cgccgagcgc cgaaggcaac cgcctcctca
     1921 tccggcgcgg aggagtgccg gtcgccgact tatcgggcgc cctgagagag ctgacccgcc
     1981 gccttcactc ggaaggtccc ggcccggtca ctcccgagtt gttcggctag ccgtccgtgg
     2041 gcgcgctttt ctcgccgccc cggttgcctt gccggccgat tctccgtatc ttgggccttc
     2101 aaattgcgat ggaaccatgc cgcgccggct gcgccggctg cccatgaagg aggatcttat
     2161 gtccgcccag gccgtttacg acgaactcac cagaaaactc agggaaatgg cgctcttgag
     2221 ttctgccgga agtgtcctgc actgggacca gcagacctac atgccgcccg aggccgcgca
     2281 gttccgcgcc gaccagctcg gtacgatctc cgggctcgtc caccgcatgt tcaccgaccc
     2341 gaaaatcggc gacatgatcg ggacggtcga gaactccgat ctcgccaagg gggatacgcc
     2401 cgaagccgcc aacattcgcg aactgcgcca tgactacgac aaaatgacca agctccccaa
     2461 cagcctggtc gaggaactca cccgcaccac cgccatggcc cagcaggtct gggccgaggc
     2521 ccggcgcaaa tccgatttcg ccatgttcct cccccacctc gaaaaggtgc tcgcgctcac
     2581 ccgccggaaa gcggacgcct acggctggaa ggacgagccc tacaacgccc tcattgatga
     2641 ttacgagccg ggggcgaacg tccgtgaggt cggcgaggtg ttcgaaagcc tccgcaaaga
     2701 actcgtggtc ctgaacgaga agatccgcaa tgccccgcgg aagccggacc acaccatcgt
     2761 ggaacgcgag tacgaggtcg agcggcagaa agtgttcggg gagtcggtgg ccacggccat
     2821 gggcttcaac ttccgcgccg ggcgcctcga tatcaccacg catcccttct gcaccggtat
     2881 cggctacggc gacacccgca tcctcacccg ctacaatccc aaccggctca acgatgccct
     2941 tttcgggatc atgcacgagg ccggccacgg cctgtacgag gcgggactcc tcaagagcga
     3001 gtactacggc acgccgatgg ccgagtcggt gtcgctcggc atccacgaat cccagtcgcg
     3061 catgtgggaa aaccaggtcg gccgctccaa atctttctgg aagtacttct tcccgcaggc
     3121 tcagcggctg ttccggaaat ccctcggcga cgtcaccctc gaccggtttt acggggcgat
     3181 caactatgtg accccgtcgt acatccgggt cgaggccgac gaggcgacct acaaccttca
     3241 catcctgctg cgcttcgaaa tcgaacgggc cctgctcacc ggcgacatga aaccggccga
     3301 tgccgcctct gagtggaacg cccgcttcga gaagtacttc ggtctgaagg tcgataaaga
     3361 cgctcacggc gtcctccagg atgtccactg gtcggccggc ctggtcggct acttcccgac
     3421 ctacacgctc gggaacctct actcggccat gttcttcgcc caggcccgca aggagatgcc
     3481 cgacctcgac ctccagttcg aacgcggcga attgctcccg ctccgggagt ggctgcggga
     3541 gaaaatccac cgccacggcc gccgctaccg ggccaacgag ctgtgccgga agatcacggg
     3601 gaaatccctt gactacaagc cgctcgtggt gtatatgaca gaaaagtatt cggaaattta
     3661 cggtttctaa cccagccaaa gagaccgaac gaaaagccgg caacctccgt gtcgccggct
     3721 ttcttttgcc cgctcgcctc gattcagtcg aatatccctt tgtacgccag gtagggaaaa
     3781 acgatgtaca ggagcgtgtc ccggatcagg tagaagaaga tgaaggcgac gatcatccgc
     3841 cagcccagcg cacggacctc cttccacgac cggggaaacc gcagcaggtt ccagcgcgct
     3901 ttgccgaccg cgggcttcgc cgggcccgcc gattccaccg ctcgtccgtc gtcactcata
     3961 cgagcgtata acagaccgct cccggccggg ttccgcggcc tcggccgcct gcgcataaac
     4021 tatttgattc cccgcccctt cgcatgcatg ttgcgggtgg cccggcgggg tcggcggacc
     4081 ccccgaacac aaacaatgga atcgtcatgc gaattgtaat catcggcggc ggggtcgtcg
     4141 gattcagcct ggccgaacac ctgctccgcg acggccacac tctgtcgttg gtcgaactcg
     4201 acccgcacct gtgccggatg atcgaggaga aactcgacct ccaggtggtc agcggatcgg
     4261 gcgcctcccc cgtgacgctc cgggaggcgg ggattgaggg tgccgacatg atgctcgccg
     4321 tgaccccgag caacgaggtc aacatcgtcg cctgctccat cgccgctctg cacgaggttc
     4381 ccagccggat cgcccgcctc cgcggccagg aattctcggc ccgcggcggc ctggtcgatc
     4441 tcacacggat ggggatcacc gccctcatcc agcccgagca ggcggtggcc gaccatatcc
     4501 tgcagttcat ggagaccccc cacgccgtcg aatcggccaa tttcgagggc ggccggatcc
     4561 tcctccgcgg ctacgccgtt aaagacacca tgcccctggc cggaaaaacc ctccgcgaaa
     4621 tccgcgccca catcgacccc gcggtcgtcc tcttcgccgc tatcgtccgg gccgggaagg
     4681 ggatgatccc cgatggcaac acccgcatcg aacccgggga catcgtctac gctctcttcc
     4741 cccgcgaatc gctcggcgtc ttcctcgacc tcgtggcgat cgagcgcaag gagagccgca
     4801 aagtgatcgt caccggcgac gctttcttca ccctcgagct ggccgaggcg ctcgagcgga
     4861 caccgcacaa ggtgaccctc gtcgacccgt cgcgcgagca tgctgagaag gccgccgcga
     4921 tgttcaaccg caccgaggtg atccatggcg actgcaccga aaccgacctc ctgcgcgaac
     4981 tgaacgtcgc cgccgcctcc ttcttcatcg ccgcatccga cgaagccgac tataacgtct
     5041 tctcgtcgct gctggccaag gccgaggggg cccgcgaggt catcgccgtg tccaccgagt
     5101 cgcgccacga ccggctcttc aattccatcg ggatcgacca cgtggtcaac ccccgcctca
     5161 tcaccgcccg cgagatcctc gagatcatat cccgcggcca catcggcgcc gtcgtgaaac
     5221 tctccgacgt cgacatcgag gccgtccgct ttaccgttga agagcccagc gaaattgccg
     5281 gccagcaggt caagcgtatc gcccgccgcc tcaagaaagg ctccatcatc ggcgtcatca
     5341 tccggggcag ttccatgatc cttcccggcg gcgacaccgt catcgaggcg ggcgaccatg
     5401 tcatcatgat ctccagccac aaaaacctga aaatgctcga ccggctcttc tcgggccggt
     5461 gaccctcatg ccggagccgt cgccatgaat gtcgccgccg tcctctacgc actgggcaag
     5521 ctgatgcagg tgatgggcgg cttcctgctg gcccctctgg ccatcgcgct gtgggacggc
     5581 cgcgtacagg ggctgccccg gctgttcgcg gccgccgatg cctccggcct cctggtcgcg
     5641 gccgtcttcg gcctcgccgt cggcaccctc ctggccgcgc tcttccggag gggccgcggg
     5701 cgccagggcc tccgcgaggg atacgccatc gtcaccatcg gctggctcac cctcaccttt
     5761 gtcgccgccc tccccctctt cctctatttc tggcgcgtgg gcatgcccga cggctcggtg
     5821 agcgggctgc gggcgttcac cgacgcctat ttcgagatca tgtccggctt ctccaccacc
     5881 ggcgccacca tcctcagcaa catcgaggcc gtcccccgct ccctcctctt cctccgctcg
     5941 ctcacgcact ggctgggcgg catggggatc atcaccctgg cgatcgtcat cttccccgcc
     6001 atgggggtca gcgcgtacag catgtaccgc ggggaggtcc ccggcccctc gcacgacaag
     6061 ctccgccccc gtctcgccca gaccgcccag gtgctctggg gagtctacgt gctgctcacc
     6121 gccctgcaga cggtgctgct gatcttcggg gggatgacct ggttcgacgc cgtcaaccac
     6181 tccttcgcca ccatggccac gggcggcttc tccacccgca acgcctcggt cgccgcctac
     6241 aattccccat acatcgaatg ggtcatcatc gtgttcatgt acctcgccgg cgtcaacttt
     6301 ctcctgcact tcaaggccct ccggggcgac ctccggtcgc tgacgcacaa cagcgagttc
     6361 atcttctatt ccgccgtcat cgccctcgca gccctggcgg ccaccattgt gctctacctt
     6421 cagggactgg agccgatggc ggaggcggcg cgcagcttcc gcgcccatcc gccgaccgag
     6481 accgctttcc tcgagcgcta tctcacggag cagtccagac tcgagggcct cgccgactgc
     6541 ttccgcgccg ccctgttcca ggtcgtctcg gtcaccacca ccaccgggtt ctcgaccgcc
     6601 gatttcgaca tgtggccgga tttcgtgcgc ttcctcctca tggtgctcat gtttttcggg
     6661 agcatggccg gttccaccgg cggcggcatc aagatgatcc gcgtcatggt cgtcgccaag
     6721 gcggccgcca acgtcgtgcg caaactcacc cagccgcgcc tggtcgcccc cgtcaaagtg
     6781 ggcggccagc cgctggacaa cgacaagatc atcagcatcg ccgccctgtt cgtcttcttc
     6841 accgggctct tcgtcctgac cagcatgctc ctgatgctct tcatccccga catgatcacc
     6901 gcggcctccg cctcgatcgc caccctcggc aacgtcggcc ccggcctggc cggcgtcggc
     6961 gcggtcgaaa actacggctg gattcccgcc ccggccaagt ggctcctggt gctctcgatg
     7021 ctgctgggac ggctcgaaat cttcaccgtc ctcatcgtcc tgcgcccggc tatctggcgc
     7081 aaatgagcgg agggaacctc cggccgcgcg catcgttatg atcgaggcaa gctgtgctgt
     7141 cggtgatccc ggcgcggcgc ccgccgccgg gaaccgggca actcaaagga tactatgcgt
     7201 tcgttctttc cgctcatctt cgccaccgcc gtgatcctcg tcttcggctc cgtcgagatc
     7261 ctcctcctgc gcctcttcaa cccttcctgg tggcgcgtgc ggtggatccg gcggaccggg
     7321 cttctgctgc cgcttgtcgg cacactctgc gccgggctgt gggggctggg cgagtactac
     7381 gccgcctcct ggctggccgc gccgggcgcc gccgccaccg ccctcgcttt cgtcctgctg
     7441 gtcgccctca tgctctcgct ccccgtctcc gggatcgtcc acttcctccg ctggacgttc
     7501 gacaagctgc gctcgcgccg ggcaaccgcc cccgacctcc ccgatcacaa ccgccgcctg
     7561 gttttcaaag cggtcgccgc cgccatcccc gcggcgaccc tgggggccgg cctcggcggc
     7621 gtggcccgcg ccttcaccgg cgtcgccgtg gcgccccgcg aattccccct ccccggcctt
     7681 cccgcgccgc tcgacggcct gcgcatcttc cacctctccg acctgcatct ccgccactat
     7741 gtcacgctcg acgatctcga ggaggtgctg cggaaagcgg aaccgctccg tcccgacctg
     7801 gtcctcgtga ccggcgatgt ggccgacgat ctcgcgctgc tgttcgatgc cctgcggatg
     7861 atcgacggct tccgggcccc gctcggcgcc ttcgcctccc tcggcaacca cgagtatttc
     7921 cggggcatcg aggaggtccg cgcgatcttc gaccgctcgc cggtccccct gctggtcgac
     7981 cggggcgtac cgctcgcggt cggcggccgc cgcctgtacg tcgccggcat cgacgatccg
     8041 cgccgcatgg gcgccaagga gcatgagttc taccggcgca cgatcgacgc cgcgctccag
     8101 gacgccgctt ccgacgatac cgtcgtcctc atgagccatc gccccgatgc cttcgactac
     8161 gcggcgcaga cgggagtctc gctcacgctc gccggccaca cgcacggcgg gcagatcgga
     8221 ctcatgggcc gctccgcctt cgaacccctc tggcccgatc gctacctctg gggcgaatac
     8281 gctctcagtg agagtcgtct ctacaccacc gcgggcgtcg gacactggtt cccattccgc
     8341 ctcggctgtc cgcaggaagc tcccgtgctc gtgctgcggc ggggatgaac gcggcgcgcg
     8401 cggctgccga tgcgccgatt ctcctatgaa aaccggcgca aaaaaaaacc cgcgcgcaac
     8461 cggaaatgtg cactttttcc ttgcaaaaaa atttcaaaca aacgtatgtt aacccgaatg
     8521 caacagtggc ccagaggaag caacgccgtc gatgaccagg agtcctttag aaagaaagca
     8581 actcgcaacg cgcaatagac cattagagaa ccactcaaac cctccatgct aaaggagcaa
     8641 cagatggcag tagcaaagaa gaaagccact gcgaagaagg cggccaagaa gaccacgaag
     8701 aaggctgcca agaagaccaa gaaggccgcc ccgaagaaga aggtggcgaa gaagtccgtg
     8761 aagaaggcgc ccgccgccaa gaagccgctc aagaagaaag ccgcccccaa gaagaaaaaa
     8821 gcggccaaga agccggccaa gaaggccgcg ccgaagaaga aagcggcgcc caagaggaag
     8881 acggcgaaga aggcgaccaa gaaggccgtc cgcaagacgg ccaagaaggc gacgccgaag
     8941 aagaagacgg ccaagaagac cgccaaaagg gccaccaaga aggcggcaaa gaagaaagcg
     9001 tctaagcgag cgccgaaccc ggcattcatg aaaccgctca ccccttcgcc ggaactcgcc
     9061 gatgtcgtcg gcagcaagcc gatgccccgg accgaggtca ccaagaagct ctgggcgtac
     9121 atcaagaaga acaacttgca ggatgccaag aacaagcgca tgatcaacgc cgacgacaag
     9181 ctgagaaagg tctttggcgg cagaggtcag gtctcgatgt ttgacatgac caagcacgtc
     9241 tccaagcacc tcaagtagtc gtcgccgatt ccgtagcatg ttcccgggcc ggtctcgcgc
     9301 cggcccgttt tctttgcccg cgccgctccg ccaaaaccat tgccgccccc gcccgccggc
     9361 gatatattct tcccaatcgt cgaacgctcg cgactatcga gaggagaccc cgtatgcctg
     9421 ccccgctcac cgcctccatg ctgcgcctcg ccgttctgat cgccgccgcc ctggcgatcg
     9481 ccgccgcccc gtccgttccc gccgcgcccc cgcccacccc ggtgcacacc gtcatcgaca
     9541 ccctccacgg cgtcgcgctg gcggatccgt accgctggct cgaaggcggc gactcggccg
     9601 ccatcgccgg ctggaccgag gcccagtgga cctacctgcg cgagtacgtg gacacctacc
     9661 cggaccgcgc caaactccac gaccgcatcg ccgcactcat gtcgatgggc agtatctcga
     9721 ctcccgaagt gcgcgacagc ctgtacttct tcaaacggcg cgacggcagc cagaaccagc
     9781 ccgtgctcta cgtccagcgc ggcctcgccg gtcagcccgc ggttctgctc gaccccaaca
     9841 ccctgtcggc cgacggcacc gccgccctgg actggtcgca cctgaccgat gacggttcgc
     9901 ttctggccta cggcgtctcc gcctccggct ccgagcaatc cgtcctctac atcatgcgca
     9961 ccgccgaccg cacccgcctc cccgacacga ttccctatac cacctcggtg agcctcgcct
    10021 ggttgcccga caatagtggc ttctactaca cccgcttccc cgagccgggg acggtgccgc
    10081 cgggggacga acgctaccac cgctgggtct acctgcaccg cctcggcgac gactggcgtg
    10141 cggactccct catcttcggc tccgaactcg acaaggcggc ctggccgggc gtggggctgt
    10201 cgccggacgg ccgccggctc ctcgtcgccg tctaccgggg atggtccgac atcgacacct
    10261 acgtccgcga ccgcaccgat cccgcctcct ccttcacccg gctctatgac gatgtggagg
    10321 cgaccgtgaa cgtcacgccc ctcgacgact gcttcctcgc cttcaccaat ctcaacgccc
    10381 cgcggtttcg cctgatgcgg gggcagtatg agaagcccgg catcgagaac tgggtggaga
    10441 tcatccccga gcgcgaggcc atcctcgaga gcgcggcccc ggtcggcggc cgcctggtca
    10501 cccgctcgct ccagaacgcc tactcggtgg tggaagtgtt cacgatggag ggccgcctct
    10561 tgcgcactct gcccaccccg tcgccgggaa ccgtctacag cgtctccggc gaactggacg
    10621 gccgcgagct gttctactac ttcagctcgt ttgacacgcc ctcggccatc taccgctacg
    10681 actttgccgc cgacaccgtc gccctgtggg cgcagctcga cgccggggtc gacctctcca
    10741 acctggtcgt caagcaggtc tggtacaagt cgcacgacgg cactccggtc tctatgttcc
    10801 tcgtgcaccg cgaggggatc cctctcgacg gctccaaccc ggctttcctc tatggctacg
    10861 gcggcttcaa cgtcaccatg accccgtatt tctcgcgcac cctggcggtc tggtacgacc
    10921 agggaggcat ctacgccctt cccaacatcc ggggaggcgg cgaatacggg gaagcgtggc
    10981 acgacgccgg caagctcggg aacaagcaga acagcttcga cgacttcatt gccgccgccg
    11041 agtacctcat cgccgagggc tacacgagtc ccgcccggct gtgcatctcc ggcggctcca
    11101 acggcggcct gttgaccggc gccgtgctgg tccagcggcc cgacctcttc cgcgccgcca
    11161 tcgtcgccgt accgctcctc gacatgatcc gctaccaccg gttcctcgcc gcccgcatct
    11221 gggtgcccga gtacggctcg agcgaggatc ccgagcagtt cgcctacctg cttgcctact
    11281 ccccttacca ccatgtgaca gagagcgcgg actaccccgc cgtgctgttg accgccgggc
    11341 tgtccgacag ccgggtcgat ccgatgcacg cccgcaaaat gacggcccgg ctccaggccg
    11401 ccgacgccac ccccgaaacc cccgttctcc tgcgcatcga gccgaaggcg ggccacggcc
    11461 agggcaaacc gatcagcaag cagattgaag aacagaccga ccagtggtcg ttcgtcttca
    11521 gagccctcaa cgtgcgcatg aaatagaatt tcccccgcgg gtcaccgggg tcaggcgcct
    11581 cctggcgcct gacggcgacc ggttggcgca atgcgaccgg cggctcatac agtcccccgg
    11641 cgcgccatcg cctgtcagcc ggagcacgcc gcgcggtcgg gcaaccgccc ggagcgcggc
    11701 cggcaacccg cctcagtccc ctttcgagga gtccgctgcg gcgtcggcgc tgtccgcagc
    11761 ggccgtgtct ttcgtgaaat cgagcgccac cgagttgatg cagtagcgca gcccggtcgg
    11821 ccgcggcccg tcggggaaaa catgcccgag gtgggccccg cactttccgc acacaacctc
    11881 tgtccgcgcc atgccgtggc tccggtcggt cttctgctca atcgcttgcg gcgacgccgg
    11941 ctgccagaaa ctcggccagc ccgacccgga ttcgtacttg gtccccgagg tgaacagcgc
    12001 attgccgcag ccgatgcagc ggtaggtgcc gtcctcgcgg tggtagtagt acttgccgct
    12061 gaagggcggt tccgtgccgc actcccgggt gatccggtac tgctcaggcg tcaattgtgc
    12121 ccgccactct tcgtcggtct tctgtatctt ctcgcccatc gcgttcccct tggctttcgc
    12181 ctgtccggcc ttggccgctg tctccttcgc cggcgcgcca gcctgtgtgc cggcaatcgc
    12241 cgagaagagg ccgagcgccg ccgcgcatgc gatcattgcc agtccggttc ttgccatcgg
    12301 tttttccttg ccgccggcgg ctgctgcggc cgccgcactg ctgtaacact ccccgctgcc
    12361 ggcggtttcc gaaaacgcgc cccatcttcc cccttgcccc cgcgcggccc ccggcgtatc
    12421 atacccgcac ggacagcact ctctgcgccg caacgactga aacgaggacg ccatgaccaa
    12481 cagcgagaat ctgctctaca tgatgcgcat aaaccagcgc gctctcaaag ctctgctcga
    12541 cgatatcacc gaggaggaat ccatggtccg cggacgcgac agcctcctgc acatccgctg
    12601 gctcaccggc cacctcgtct acaacgtcaa ccacgtcctg gccatgatcg gctcgctgcc
    12661 ggtgatgctc ccgtcctcct acaagcagct ctttgaccgg ggcagcgcca tcaccaatga
    12721 tcccaaagcc tatccgtcgc tggccgcctt gcgcagcgaa ctgctcgatc tgcacgaccg
    12781 cctgatcgac gttctccccg gcgtgtccga tgacgacctc gacgagccgc tgccgaaaga
    12841 cgccggtttc aacgctgttc gcctgaacgc cgcctccttc atgtgcatgc acacgttcta
    12901 ccactgcggc cagatcgcca tgatccgccg cgtcctcggg cgcgagcggt ctttcggatg
    12961 agggtcgcgg ccgcgggccg cccccgctcc cgccccgccc gccgcgagca ccctcggccg
    13021 cgctgtgcaa atcccggccg ctttctgccg caccggcgcc gggtgtgcgc atgactgcgc
    13081 accgcgatgc aggagatcgg acgcccgggg tgaagtccgg ttctctgtgc aatctctgat
    13141 ctcgcctcca accaacctgc ggtcaaccac ttggcccccc tggcccctcc ctttccgact
    13201 tggcaccgcg tttgccctca tggcccccac gtaggccata gctcaaccgc agtgaaaggg
    13261 agtttgagaa atgatgaacc gcacgcaaag caacagtcag agaggcagcg cactcttaat
    13321 cgcgctggtg ctcaccggga tgctctcgct gatcgccctg atggcgatcg acaactcgca
    13381 gaccgagatc gacctgtcct tcaacaagat caattccgac cgctccttct atattgcgga
    13441 agcaggcgtg cagcacgccc acaccgtgat caacgccacc cccggctgga atgccgggct
    13501 ggccggcgtc gccttcgccg gcggcgcgta ctcggtgtcg gtccgcgaca gcctgagcga
    13561 acccgccctg gccgacagcg tcgtcctgct ctcccagggg gaggtcaacg gcgcccgctc
    13621 caccatcgaa gccaccctcg cccccgagcc gttctacccg ttccgctatg gcctctttgc
    13681 cgacagcggc gtggcgatcg gcaacagcgt cagcaccgac tcctggaact ccgattcggc
    13741 cagctacgcc ggttcggcgg tcaacgccta cggatcggtc ggatccaacg gccccattgt
    13801 gatcgagaat aacccgacca tcagcggcga tgtctacacg gcccaggccg gcgccatcac
    13861 catcaccggc accggccgcg tgctcggcga cacctcctcc caggcgccgg aacttgattt
    13921 cagcaagctc atcagcccga cccagttcgc cgacgccgaa gccgtcaacc gcgcccccct
    13981 cggcatgtcg ggcagcttca cctacaacaa cggcagccgc gacctccgca tcggcgccgg
    14041 ccagaccatg gtcctcgaga gcggcaccta cgtccttgat gacattctgc tggagcagtc
    14101 ggccaagctg gtgctcgccc cgggcgcgca ggttgagctc tacgtgaccg gcaacatcga
    14161 cctgcgcaac aaagccaccg tcaactc
//
LOCUS       DAOPOE010000084        13308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5413_length_13308_cov_5.618501, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000084 DAOPOE010000000
VERSION     DAOPOE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13308
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5413_length_13308_cov_5.618501"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PKM94_10685"
     CDS             <1..1194
                     /locus_tag="PKM94_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOD67139.1"
                     /translation="TPLSVIPDLLLGDDTVYPGQAFPVYGNDINGNGSPELIAGHYDN
                     ALFPSLLFFELNQDLDSTVDFQLWAPEHLDYPVFGRAILTGDFNGDGWEDLVTNMLFS
                     AQAGVSDQVLLYWGGPAFDTLPDANIVRSMTYDPEYISFGQRLEHLGDLNGDGWDDFL
                     INYGEGGSEDSCSWVHFGGSDFDTVPDITICDWALEARLAGDINNDGYDDLIKGRSTS
                     FQAIGQANIYLGGPNVDSIPDVVIDHSMFSGYYNYFGHEVAGLGDVNGDGIDDFAVAS
                     SRGAGFPWVIFIFAGWDDAVSVDDPLDPEPALPTNFTLDQNYPNPFNPNTTIRFSLPH
                     AEMVSLRILNALGQTVAVLVDQQRLGAGEHEVLWDATEYASGVYLYELEYPAGKQSRK
                     MVVLK"
     gene            complement(1261..3081)
                     /locus_tag="PKM94_10690"
     CDS             complement(1261..3081)
                     /locus_tag="PKM94_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD67140.1"
                     /translation="MVRFTVQLFGVTVFALGLIWFVGSGEAQQSDPKEPVLWADTFHI
                     NLEETPDSAALESRRQDRDSFLMDVKKLTHTALNIRNQYMEEVNTEEIIKAGINGMLS
                     DLDRFSVLMEKSSYDALMESTHGKYEGLGMQIDERNDRIVIISPIEGTPAYNKGLRAG
                     DIIWAIDGKSTEGMTSAEASKMMRGKAGTSIKLAIKRNGIEEPIEFELERALIELKSV
                     NYAGVIPGTTIGYVRLSRFAEETSHELRQAISDLNGKGITALVFDLRSNGGGLLDQAK
                     ETAELFLQEGREIVYTKGRFENTERHYRAERPPLFPPDKPLVILVDEGTASASEIVAG
                     AVQDWDRGLIVGNTTYGKGLVQQIFPISSDGSMALKLTTAKYYVPSGRCIQKPETQAK
                     PGSHAALEDEETEAAPEKPADSLAVSSEEIYYTNGGRVVYGGGGIVPDIEIARETWKP
                     IEINLYQSGLFFNYAVQYVADHPSVKPDVEVTDGMLDDFRQYIKKEKFDYTSSLEASL
                     EKLRETAEREKKVDLLAEVLDSLNTLVELEKSHDFDESKDYIRRTIKREIVSAIAGQT
                     GVYEEIVLRTDDAVRKAVEILQSGQYTKLITEGQKKAQAD"
     gene            3424..4215
                     /locus_tag="PKM94_10695"
     CDS             3424..4215
                     /locus_tag="PKM94_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67141.1"
                     /translation="MTGNRPKFRNSPAAALLLHLLAPGFGHFYWREYLFGVFIFLVLL
                     MAGALFVLSLLIDLPFYGRLIIYGLPIIFYLISFGDLYRIARREHTARPQTSRRALVI
                     WLLAAAYELVWPLAPLNFALLNAPSVYVMRENNLAPVFRDGDLLKASRLAYTFHTWFV
                     NKPIVHALPDRFAVVRVTAEGRALTGIVLGLPGEEVEVVDGVVVVDGTPQVVPTTASR
                     LLNGDYPLTQAGGYSLLIALPEYGRIARVYEIPLGDLIGKVEKVL"
     gene            4463..5128
                     /locus_tag="PKM94_10700"
     CDS             4463..5128
                     /locus_tag="PKM94_10700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD67142.1"
                     /translation="MSRIKNLIFDLDGTLIDSSEGVVAAFHYAFAAAGLPLPPAEEVK
                     RFIGYPLETMFAHFTDYSPERLRTYFRERAAETIVASTVPLPEVEPTLAALRDRGFRL
                     AIATTKIVANIEGILDKLDWRGVFEAYAGGNEVQAVKPDPEIFRLVLARLGAVPRESL
                     VIGDTINDILAAQAVPLPVIAVASPWEPAGRVLAACPDFFLDSISQLPRVLDEINTRR
                     SPG"
     gene            complement(5275..5934)
                     /locus_tag="PKM94_10705"
     CDS             complement(5275..5934)
                     /locus_tag="PKM94_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD67143.1"
                     /translation="MKLRIVQKHIAELDLVPWVEAADRAGMDLVCFGELAATGCLYEP
                     RPVEPVDRILQRLVPFGVGVMFGAVRVEVEGRYNSYFFRRHRQTWVYDKINLFPPFGE
                     DRIYRPGKVPGLFVTPFGDFGVAVCYDLRFDDIFARLAELAVPYLIVPAAFPQVRIED
                     WRRLLAQRARELGATVIGVNAVGSDGVNRFGGSSMVVAPDGSIVAQADETGEQILDVT
                     L"
     gene            6396..8735
                     /locus_tag="PKM94_10710"
     CDS             6396..8735
                     /locus_tag="PKM94_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD67144.1"
                     /translation="MVLLCAIAAVLSVAAPAGSVTVRLGYFEAGEHPLHYVLRDEFIR
                     QLENMAPADTQFVFIPNGYRSASWIRDSSRVMAASLARQGGIDILVAIGPWVVEDLLA
                     AGYAGPILGVRQIDPFGAGLIDSGGRPAAPNLTVQDMPGKIEDDIGVLRAIAPVKKLG
                     VLWFDTGPERDSIIARAERLGAQAGFETVTADGYDVNGTYAFFKAFSALPPDIDGLYI
                     GPTWGMTVTEAADFFRMVMGRKVPLMTWEGRYQVTRGAFATNYAYGLVSEAHFQALKA
                     LRIARGERPADLPVRFRGGSALALSGPAIRRLELQVPAEALLSGELIDDEPPPATEWY
                     TLADAVGRALRSNPSYLATYDAIEAAVQGARAAGAGYLPQVSVEGQAGVVDHHTTHNT
                     VEPLGRQQARASLVLDQTIFSPALLKARKAAGQQERVAAAAQRQARLDLELAVETACL
                     GYLQALDEQRVERRYRELVERTLEYAAAEFYLRDEREPDLSRWQDERNDAITRVLEAE
                     NNVYTGRVLLNLLFNLPPERPVALDTNLVTARQVVGADYEALSPLAVQPDRRQQFESY
                     LVDQARAASPTLAGEDARLEVQRTRVDEATARRWPTLDLRAAFNIADEIDDDRGIIDE
                     RTDTWSVFGRMKLPLYLGGAVGKERARLKASLSEMEYRRDARLLEVMANIRRETGRLI
                     TNIAALTRHGRSVELALHAMGLIINDYEAGKRDMTALLEAYRHARTAELTAIGTRYEY
                     LRAMARLVHTVGWSPSENLRAFPEEFRERAREGLAPAGQ"
     gene            complement(8942..9991)
                     /locus_tag="PKM94_10715"
     CDS             complement(8942..9991)
                     /locus_tag="PKM94_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD67145.1"
                     /translation="MKTVFLLGVAAVALLAGGALAGDYHTGTTLNCAECHVMHYSQTH
                     GYNADGSGITVGLGADGPYHFLLRNEVNTLCLSCHDNQTFAPDVLAENGGNVTANGRQ
                     AGALNRDNVAPYYDAAGHTLGSTDVAPGGTFSNDHGLNCIDCHTQHGRTSGGQSIYRN
                     ANIFINGTPATPYMTYAIGTNDLTKDIFELSATYGGNHYDMQNVQFNEPNVTASGYGA
                     FCQQCHTDFHGSSSSANMRNTAAPTQTEWLRHPTADANIGALTGGHSSLSKFTGQAYR
                     VQVMSATGNWGTWGAAWTGAPTTLTPSCMSCHKAHGNTRAFGLIWATGEANPGENGDG
                     TNYKILCGQCHVQAP"
     gene            complement(10433..11422)
                     /locus_tag="PKM94_10720"
     CDS             complement(10433..11422)
                     /locus_tag="PKM94_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67146.1"
                     /translation="MNRNRLLCLPGAALAALLAGAAPSASAQSAGDELNARISITALA
                     ADDGWDQPLRSPMALFVDPHAQEVFVADAGNNRVVVYDALLQPKYSFLHFVPDPASGR
                     AVKGQPRDLVVNSRGEIILIDTHSECLDVLDFRGALLERLRLDLLLGDTALSLRPQAV
                     ALGPGDRTIVATAGDVAGVLILDQTFALERTIGVDDPAQSPFRTIMALTVDDSTLYVT
                     DLYAEPAVKLFDLSGRYLRGFAGHDVDRADLSFPSGIAVMTDTAGEKTVWVTDGLRQV
                     VKVYRPNGAFLAFVGGFGLKPGEFRYPGDIAFNGTAFYVLERAGGRVQRFVID"
     gene            complement(11412..11993)
                     /locus_tag="PKM94_10725"
     CDS             complement(11412..11993)
                     /locus_tag="PKM94_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67147.1"
                     /translation="MALFRLLLAGLMLVLGGCASQMTAVPRVEELLPAGARVAVLPFE
                     NLSGTESAAEIVTSHFQTLLAGLGRIDLAEHGDVYAGLRQYRIRSATRITRAEIDSLC
                     ALLRLDYLLVGSVLEFDQRDDRFLGVVPTVSYNCRLLDGKTGETVWVAANNGRGDKGE
                     IVFGIGAIRSADQLALAMVKKTVAEIGGLFHEP"
     gene            complement(11998..12597)
                     /locus_tag="PKM94_10730"
     CDS             complement(11998..12597)
                     /locus_tag="PKM94_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67148.1"
                     /translation="MNRTISTFVRLLVGAAFLALAAGCTVGRLYVNREADMGFYSKVG
                     VLTFANLTSDQNAAEKVTASFITELLMLDAVSVANAGDFNKAASETIKGDFANALEDL
                     TSEQAFQLGQAAGVEGIFVGAVSQYGMVRSGQTEFPPVAVIVRFVDCQTGAVVWSYET
                     SRKGGPKFPIFSFGETHTLGEMTTKVCREVAGAFGQIAR"
     gene            complement(12594..>13308)
                     /locus_tag="PKM94_10735"
     CDS             complement(12594..>13308)
                     /locus_tag="PKM94_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67149.1"
                     /translation="TRLTFNRTIFQQSERTESVLDGEGDVVGLADDGDYVLDTYNAAL
                     NFKPLPYIRTYLDLSLTRNSDPVDPNRRYQFTRSLDARVDFSRRLEGRFTVTALSEGG
                     VLKLGSAFSQNYNAGLTYIPRDNLNMNLTVIRSTYTGAVESRTTSLTGYMSYSFRRAF
                     SVYASYNRRRESRERAISLPGGVAEVDQRPATVNGQLIMYLSPRTTLSLAYIRNETDN
                     TGGLQTVDESFQTVVTLQI"
BASE COUNT         2322 a         4061 c         4419 g         2506 t
ORIGIN      
        1 actcccctta gtgtcattcc agatttgctt ttaggtgacg acactgtgta tccggggcaa
       61 gccttccccg tgtacggtaa cgacatcaac ggtaacggtt cgcctgagtt aatcgctggc
      121 cattacgata acgctctctt cccctccctg ctgtttttcg aacttaatca ggatttagac
      181 tcgacagtag attttcaact atgggcacct gaacacctcg attatccggt attcggtcgc
      241 gcaattctaa cgggtgactt caatggcgac gggtgggagg acttagtcac gaacatgcta
      301 tttagtgccc aggccggcgt aagtgatcag gtgcttctat actggggggg gccggccttc
      361 gacacactgc cagacgccaa tattgtccgg agtatgacgt acgatcccga gtacattagc
      421 tttggtcagc gcctcgagca cttgggtgac cttaacggcg acgggtggga tgattttctc
      481 atcaactatg gggaaggcgg ttccgaggac tcatgctcct gggttcactt tggcggctca
      541 gactttgata ccgtacctga catcaccatc tgcgattggg cattagaggc ccgcctggcc
      601 ggtgacatca acaatgacgg gtacgacgat ctcatcaagg ggcggtcaac ctcatttcag
      661 gctatcggtc aggccaatat ctaccttggt ggaccaaacg tcgacagcat ccccgatgtc
      721 gtcattgacc actccatgtt ttccggctac tataactatt ttggacatga ggtggccggg
      781 ctgggcgatg tcaacgggga tggtatcgat gactttgccg tggcgtcgtc gcgaggagcc
      841 ggtttcccct gggtcatctt catctttgcc ggctgggacg atgccgtcag cgtcgatgac
      901 cccctcgacc cggaacccgc cctccccacc aacttcacct tagaccaaaa ctaccccaat
      961 cccttcaatc cgaacacgac gatccgcttc tctttgcctc atgctgagat ggtcagcctg
     1021 cgcatcctta acgcccttgg ccagacggtg gcggtgctgg tggatcagca gcggctcggc
     1081 gccggtgagc acgaggtgct gtgggacgcc accgagtatg ccagcggggt gtacctgtac
     1141 gaactggagt acccggccgg gaagcagagc cggaagatgg tggtgctcaa ataacccccg
     1201 taaaaaaacc ggcgcctcct acgagcgccg gtttccgaac tctcatccct gcgtcggcga
     1261 ctaatccgcc tgggctttct tctgcccctc cgtgatcagc ttggtgtact gaccgctctg
     1321 gagtatctcg accgccttgc gcacagcatc gtcggtcctc agcacgatct cctcgtatac
     1381 cccggtctgc ccggcgatgg ccgacacgat ctcccgcttg atcgtccggc ggatgtagtc
     1441 tttcgactcg tcgaagtcgt ggctcttctc cagctcgacc agggtgttca ggctgtcgag
     1501 cacctcggcg aggaggtcga ccttcttctc gcgctcggcc gtttcccgca gtttctccag
     1561 cgaggcctcc agcgacgacg tgtagtcgaa cttctccttc ttgatgtact gccggaaatc
     1621 atcgagcatc ccgtcggtca cctcgacgtc cggcttgacg ctcgggtggt cggcgacata
     1681 ctggacggcg tagttgaaga acagccccga ctggtacagg ttgatctcga tcggcttcca
     1741 ggtttcgcgg gcgatttcga tatcaggcac gatgccgccg ccgccgtaga caacccggcc
     1801 gccgttcgtg tagtagattt cctccgacga gacggcgagg gagtcggccg gcttctcggg
     1861 cgccgcctcg gtttcctcgt cctcgagggc cgcgtgcgac cccggcttgg cctgtgtctc
     1921 cggcttctgg atgcaccgtc ccgagggcac gtagtacttg gccgtcgtga gcttgagcgc
     1981 catgctccca tccgacgaga tcgggaaaat ctgctgcacg agccccttgc cgtaggtcgt
     2041 gttgcccacg atgaggccgc ggtcccagtc ctgcacggcg ccggcgacaa tttccgaggc
     2101 tgaggccgtg ccttcatcga ccaggatcac cagcggcttg tccggcggga acagcggcgg
     2161 gcgctccgcg cgatagtggc gctcggtgtt ctcaaaacgc cctttggtgt agacgatctc
     2221 gcgcccctcc tgcaagaaca actcggccgt ctccttagcc tgatcgagca gtcccccgcc
     2281 gttggagcgc aggtcgaaca ccagcgccgt gatccccttg ccgttgagat cgctgatcgc
     2341 ctggcgcagt tcgtgcgagg tctcctcggc gaaccgcgag agccgcacgt acccgatcgt
     2401 cgtccccgga atcaccccgg catagttcac cgacttcagt tcgatcagcg ctcgttcgag
     2461 ctcaaactcg atcggctcct cgatcccgtt gcgcttgatc gccagcttga tcgacgtccc
     2521 ggctttgccg cgcatcattt tcgacgcctc ggccgaggtc atcccttccg tcgacttccc
     2581 gtcgatcgcc cagatgatat cccccgcccg gagacccttg ttgtaggcgg gcgtcccctc
     2641 gatcggggag atgatgacga tgcggtcgtt gcgctcgtcg atctgcatcc ccagcccctc
     2701 gtacttcccg tgcgtcgact ccatcagggc gtcgtacgag ctcttctcca tgagcacgct
     2761 gaagcggtcg agatccgaga gcatcccgtt gatgccggcc ttgatgatct cctcggtgtt
     2821 gacctcctcc atgtactgat tgcggatatt gagcgccgta tgggtgagct tcttcacgtc
     2881 catcaggaag gagtcgcggt cctggcggcg gctctcgagg gcggccgagt cgggcgtctc
     2941 ttccaggttg atgtggaagg tatccgccca gagcaccggt tctttggggt cggactgctg
     3001 ggcctcgccg gagcccacga accagatcaa gccgagcgcg aatacggtga cgccgaagag
     3061 ctgaactgtg aagcgaacca tacggcctcc agactcattg agagtgacga acacaatgta
     3121 tcgtaaatcg gagacttgtc aagtttgcgg actttttccg aagtacgctg tatctgcctg
     3181 caaattctac gcttaagccg gcgcagcggt tgcaccgaaa ctgaccaccg gcggatggcg
     3241 cataaataac ttcttgcctg ccgactgctt agcacgttaa taccactgta tcggtcatcg
     3301 gcaaccgttc ccggaatcgg ggcggcagtc gcgcgggcaa cgcaacacgt ttcccgataa
     3361 ggtattagct gcaagactgg acgttgtacc ggcaacaaca ttgttgcctt tctgaacagt
     3421 agaatgacgg gcaatcgacc aaagttccgc aactcccccg ccgcagccct tcttctgcat
     3481 ttgctggctc cggggttcgg ccatttttac tggcgggagt acctcttcgg cgtcttcatt
     3541 ttcctggtcc tcctcatggc cggagcgctg ttcgtcctct cccttctgat cgatctcccg
     3601 ttctatggcc ggctgatcat atacggcctt ccgatcattt tctatctgat ctctttcggc
     3661 gacctctacc ggatcgcgcg gcgcgagcat accgcccgtc cgcagacgag ccgccgggcg
     3721 ctggtgatct ggttgctggc cgcggcctat gaactggtct ggccgctggc cccgctgaat
     3781 ttcgccctcc tgaatgctcc gtcggtctat gtcatgcgcg agaacaacct cgccccggta
     3841 ttccgcgatg gcgacttgct gaaagcttcc cgcctcgcct acacctttca cacctggttt
     3901 gtcaacaaac cgatagttca cgctctaccg gaccggttcg cggtggtccg ggtcacggcc
     3961 gagggccggg cgctcacggg gatcgtcctc ggcctccccg gcgaggaggt cgaagttgtc
     4021 gacggggtgg tggtggtcga cggcactccg caggtcgttc cgactaccgc gagccgcctg
     4081 ctcaacggcg actatccgct cacacaagcc ggcggctact cactcctgat cgcgctcccc
     4141 gaatacggcc gcatcgcccg ggtttatgag attcccctgg gcgacctgat cggcaaggtg
     4201 gagaaagtat tgtgaacgcc acggccgcca tgtggcggga gcacagcgcg agatgcgctc
     4261 gaggtctgga tcttcgtggg ccggacgctt ttcttccgtt ccgcaaacac gcccgtcagg
     4321 accatgaagg ccctgagcta cagaaatccg cgatgactca gtgccctctt gtaggtcagg
     4381 acccttgtgg tcctgacgct cttcttccgt tccgcaaaca cttccggcgt gaccgccact
     4441 atccggaggc gggctactgc cgatgagccg gatcaagaac ctcattttcg acctcgacgg
     4501 caccctgatc gactcctccg agggcgtcgt ggcggcattc cactacgcct tcgctgccgc
     4561 cggccttccg cttccgcccg ccgaagaagt caagcggttt attgggtatc cgctcgagac
     4621 aatgttcgcg cacttcaccg actactcccc cgaacgcctg aggacgtact tccgcgagag
     4681 agccgccgag acgattgtcg cgtcgacggt cccgctgccg gaggttgagc cgacgctggc
     4741 ggctctccgc gaccggggtt tccgcctggc cattgcgacc accaagatcg tcgccaacat
     4801 cgaggggatc ctcgacaagc tcgactggcg gggggtgttc gaggcctacg cgggcggcaa
     4861 tgaagtgcag gcggtcaaac ccgacccgga gattttccgc ctggtcctcg cgcggctcgg
     4921 cgccgttccg cgagaatcgc ttgtgatcgg cgacacgatc aacgacatcc tcgcggcgca
     4981 ggcggtcccg ctgccggtga tcgcggtggc ctcgccgtgg gaaccggccg gccgagtcct
     5041 agccgcctgc ccagatttct tcctcgactc gatctcccag ctcccgcgtg tgctcgacga
     5101 gatcaacacc cgcagatcgc cggggtgacg gttgctgcgc acgagagagt tgggcgcaca
     5161 gccggcccga acagacgtgt ccccctgagc ggagccgggg tctcacacgg gcggcgcagt
     5221 gcctccaaga atagtcccac gcggcttcct gctcgcggcg attcggtcgg ggagctacaa
     5281 cgtcacgtcg aggatctgct cgccggtttc gtcggcctgg gccacgatgg aaccatccgg
     5341 cgcgaccacc atcgaggaac cgccgaaccg gttgacgccg tcgctgccga cggcgttgac
     5401 gccgatcacg gtggcgccca gttcgcgggc gcgctgcgcc agcaagcgcc gccagtcttc
     5461 gatgcgcact tgcggaaagg ccgcggggac gatcaggtac gggacggcga gttcggcgag
     5521 gcgggcgaag atgtcgtcga aacggagatc atagcagacg gcgacgccga aatcgccgaa
     5581 gggcgtgacg aagaggccgg ggaccttccc cggccgatag atgcgatcct cgccgaacgg
     5641 cgggaagaga ttgatcttgt cgtagaccca ggtctggcgg tgccggcgga agaagtagga
     5701 attgtaccgg ccctccacct cgacgcggac ggcgccgaac atgacgccca ccccgaacgg
     5761 cacgagccgc tgcaggatcc gatccaccgg ttccaccggc cgcggctcgt agaggcagcc
     5821 ggtggcggcg agctcgccga agcagacgag gtccattccg gcccggtcgg ccgcctcgac
     5881 ccacggcacg agatcgagct cagcgatgtg tttttgtacg atacgcagct tcacggcggc
     5941 tctcccggcg tccacagaca atacgggaca gggaacgttt gcgttaaggt agggggcgca
     6001 acgcgccagg caagtaaata gcgcgggagg cggaaaaacc gacttaacag aaggagtcgg
     6061 cggaaagggg gtaccccaca gctctgcggt gggggctttg cgttattcac tcacagtcat
     6121 gttcaccatg tccacccgca cgggcacgta gtgccttccc aggcaggaat tgtagctcga
     6181 ccatttccag tcggcagggc aacggcccac cgcagagctg cgggcgacca gccgctcgcc
     6241 ttgcggccgg cgagggggtg cgacatcggc ggaagccgcc cgccggccgt tgaaaaatac
     6301 ctttaaaccc ggccggcttt tctctattat cccggtacaa tcggaaaacc gtgctccgga
     6361 gatgtgggtg tatgcgccga ggtgagcaag ttgtgatggt tcttctgtgt gcgatcgcgg
     6421 cggttctgtc tgtggccgcg ccggccgggt cggtcactgt ccgcctgggc tatttcgagg
     6481 cgggggaaca ccccctgcac tatgtgcttc gcgacgaatt catccgccag ctggagaaca
     6541 tggccccggc cgacacgcag ttcgtattca ttccgaacgg ctaccggtcg gcctcgtgga
     6601 tacgggacag cagccgggtg atggcggcga gcctggcgcg ccaggggggg attgacatac
     6661 tggtggcgat cggaccgtgg gtggtcgagg accttctcgc ggccggctac gcggggccga
     6721 ttctcggggt gcgacagatc gatccgttcg gcgccgggct gatcgactcc ggcgggcggc
     6781 ccgcggcgcc caacctgacg gtgcaggaca tgccggggaa gatcgaggac gatatcggcg
     6841 tgctgcgggc gatcgcgccg gtgaagaagc tcggagtgct gtggttcgac acggggccgg
     6901 agcgggattc gatcatcgcg cgggcggagc ggctgggcgc acaggccggg ttcgaaacgg
     6961 tgacggcgga cggctacgac gtgaacggta cctatgcctt cttcaaggcg ttttccgcgc
     7021 tgccgccgga tatcgacggt ctgtatatcg ggccgacctg gggaatgacc gtcaccgagg
     7081 ccgccgattt cttccgcatg gtgatgggcc ggaaagtccc cctcatgacg tgggaggggc
     7141 ggtaccaggt cacgcggggg gcgttcgcga ccaactacgc ctatgggctg gtttcggagg
     7201 cgcatttcca ggcgctcaag gcgctgcgga tcgcgcgcgg cgagcggccg gccgatctcc
     7261 cggtccggtt ccggggcggg tcggcgctgg cgctcagcgg tccggcgatc cggcggctcg
     7321 aactgcaggt gccggccgag gcgctgctgt cgggcgaact gatcgacgac gaaccgccgc
     7381 cggcgaccga atggtacacg ctggccgatg cggtggggcg ggccctgcgg agcaatccgt
     7441 cctacctggc gacctacgat gcgatcgagg cggcggtgca gggcgcccgg gcggccgggg
     7501 ccgggtacct tccgcaggtg tcagtcgagg ggcaggccgg ggtggtcgat caccacacca
     7561 cccacaacac ggtcgaaccg ctggggcggc aacaggcgcg cgcttcgctg gtactcgacc
     7621 agacgatctt ctcgccggcg ctgctgaagg cgcggaaagc ggcggggcag caggaacggg
     7681 tggccgccgc ggcccagcgc caggcgcgcc tcgatctgga actcgcggtc gaaaccgcct
     7741 gcctcggata cttgcaggcg ctcgacgagc agcgggtgga gcggcgctac cgggagctgg
     7801 tcgagcggac gctcgagtat gcggcggccg agttctacct tcgggatgaa cgcgagcccg
     7861 acctgtcgcg ctggcaggac gagcgcaacg acgccatcac gcgggtgctg gaggcggaga
     7921 acaacgtgta caccggccgc gtgctattga atctgctgtt caaccttccg ccggagcggc
     7981 cggtggcgct ggacacgaac ctggtgacgg cccggcaggt ggtgggcgcc gattacgagg
     8041 ccctctcccc gctggcggtt cagccggacc ggcggcagca gttcgaatca tacctcgtcg
     8101 atcaggcgcg ggcggccagc ccgacgctgg ccggcgagga tgcccggctg gaggtgcagc
     8161 ggacgcgggt ggacgaggcg acggccaggc gatggccgac gcttgatctg cgggcggcgt
     8221 tcaatatcgc cgacgagatc gacgacgatc gggggatcat cgacgagcgc accgacacct
     8281 ggtcggtgtt cgggcggatg aaactgccgc tgtatctcgg cggggcggtg gggaaggaac
     8341 gggcgcggct caaagcgagc ctgagcgaga tggagtatcg gcgggatgcg cggctgctgg
     8401 aggtcatggc gaacatccgg cgcgagaccg gacggttgat cacgaatatt gccgcgctca
     8461 cccgccacgg gcggtcggtt gaactggcgc tccacgccat ggggctgatc atcaacgact
     8521 acgaggcggg aaaacgggat atgacggcgt tgctggaggc ctaccgccac gcccgcaccg
     8581 ccgagctgac cgctatcggg acgcgctatg agtatctgcg ggcgatggcg cggctcgtcc
     8641 acaccgtggg ctggtccccc tcggagaacc tgcgggcttt tcccgaggag ttccgggagc
     8701 gggcgcggga gggactggcg ccggcggggc agtagtcttt tccggtcgcg gcggcggccc
     8761 cggatcacta tcacacttcg agtacgccga gcgcgtgctt tccccctggg ccttggcggg
     8821 ccctcgtttt cggaagggag gagagtggat accatgcgcc ggtctttggc tgtgtcggac
     8881 ccggccaaaa aagaaggaga gcccgcgggc tctccttccc taattcagcg gaaaaacggg
     8941 atcacggagc ctgcacgtgg cactgaccgc agaggatctt gtagttggtg ccgtcaccgt
     9001 tctcgcccgg gttggcttcg ccggtggccc agatgagacc gaaggcgcgg gtgttgccgt
     9061 gcgccttgtg gcagctcatg cagctcgggg tgagcgtagt cggagcgccg gtccaggccg
     9121 cgccccaggt gccccagttg ccggtcgccg acatgacctg cacgcggtag gcctggccgg
     9181 tgaacttgct gaggctggag tgcccgccgg tgagagcgcc gatgttggca tccgcggtgg
     9241 ggtggcgcag ccactcggtc tgcgtcggag cggccgtgtt gcgcatgttg gcgctgctcg
     9301 acgaaccgtg gaagtcggtg tggcactgct ggcagaacgc gccgtagccg gacgcggtca
     9361 cgttcggctc gttgaactga acattctgca tatcatagtg attgccgccg taggtcgcgg
     9421 acagctcgaa gatgtccttc gtcagatcgt tggtcccgat ggcgtaggtc atgtacgggg
     9481 tcgccggagt gccgttgatg aagatgttgg cgttccggta gatgctctga ccgccggagg
     9541 tgcgaccgtg ctgcgtgtgg cagtcgatgc aattcagacc gtggtcattg ctgaacgtgc
     9601 cgcccggagc cacgtcggtc gagccgagcg tgtggccggc agcgtcgtag tagggagcga
     9661 cgttgtcgcg gttcagggcg cccgcctggc gaccgttggc cgtcacgttg ccgccgttct
     9721 ccgccaggac atccggagca aacgtctggt tgtcatggca cgagaggcag agcgtgttga
     9781 cctcgttgcg cagcaggaaa tgataaggac catcggcccc aagcccgacc gtgatgccgg
     9841 aaccgtcggc attgtagccg tgggtctggc tgtagtgcat cacgtggcac tcggcgcagt
     9901 tgagggtcgt gccggtgtgg tagtcgccgg ccagcgcacc gccggccaga agcgccaccg
     9961 cggcaactcc gagcaggaag acagtcttca tgctgctttc ctccagtttc gtgaaagcat
    10021 acttccacgg ggtggaatgg tgatagtatg ggtgcattgt gagtgattgg gcgaatccga
    10081 agtttggaga cctgcgtgat ctatatttgt gattcctgtc acaagcgatg gtcgaatcat
    10141 tccggtggtt ggccattctc gtttccaagt ttctcgatca gggaacttct cagtgcgtag
    10201 acctaagttg gttcatccgg tgatctgcgt tcataactgg gcaaaggata tcaataaagc
    10261 tcctatttgt caagaactaa atgacactta aatgacacaa aatatcatca ttgctccgaa
    10321 atgagacgtt aagccattcc ctaacatcta cttgaaagta cagattcgtc ccggcatatg
    10381 tgtacatatt ggcagggatt agatccggag aatcccgata ttctgggtta tcttagtcga
    10441 tcacaaagcg ttggacgcga ccaccggctc gctccagaac gtaaaacgcg gtaccattga
    10501 aggcaatatc cccagggtag cgaaactccc ccggcttcag gccgaaaccg ccgacgaacg
    10561 ccagaaacgc tccgttcgga cggtacacct tgaccacctg ccgcagaccg tcggtcaccc
    10621 aaacagtctt ctcccccgcc gtgtcggtca tgacagcgat gccagagggg aaggacaggt
    10681 cggcccggtc gacatcgtgg ccggcaaaac cgcggaggta ccggccggag agatcgaaga
    10741 gtttcacggc cggctcggcg tagaggtcgg ttacgtagag ggtgctgtca tcgacggtga
    10801 gggccatgat ggtgcggaac ggggactggg cggggtcatc gacgccgata gtccgctcca
    10861 gggcgaaagt ctgatcaagg atgagcaccc ccgcgacgtc gccggcggtc gccacgatgg
    10921 tgcggtcgcc gggtccgagg gcaaccgcct gcgggcggag ggagagcgcc gtatcgccca
    10981 gcaggaggtc gaggcggagg cgttcgagca gggcgccgcg aaaatcgagc acgtcgagac
    11041 actcgctgtg ggtgtcgatg aggatgatct cgccgcggct gttgaccacc aggtcccgcg
    11101 gctgcccctt tacggcccgc ccgctcgcgg ggtcggggac gaagtggaga aacgagtact
    11161 tgggctggag gagggcgtcg tagacgacga cgcggttgtt gccggcatcg gcgacgaaca
    11221 cttcctgcgc gtgggggtcg acgaagaggg ccatggggct gcggagcggc tggtcccagc
    11281 cgtcatcggc cgccagcgcg gtgatcgaga tgcgggcgtt gagctcgtcg ccggcgctct
    11341 gcgcgctcgc ggagggggcg gcgcccgcca ggagggcggc gagggcggcg ccgggcaggc
    11401 aaagaaggcg gttacggttc atggaacagc ccgccgattt cggcgaccgt tttcttgacc
    11461 atcgccagag ccaactggtc ggcggagcgg atggcgccga tgccgaacac gatctcgccc
    11521 ttgtccccgc ggccgttgtt cgcggccacc cacacggttt caccggtctt gccgtcgagc
    11581 agacggcagt tgtaggagac cgtggggact accccgagga agcggtcgtc gcgctggtcg
    11641 aactcgagca ccgatccgac caggaggtag tccaaacgga gcagggcgca gaggctgtcg
    11701 atttcggcgc gcgtgatgcg ggtggcggaa cggatgcggt actggcggag gccggcgtag
    11761 acgtcgccgt gctcggccag gtcgatgcgg ccgagaccgg cgaggagggt ctggaagtgg
    11821 gaggtgacga tttcggcggc gctctcggtt ccgctgaggt tttcaaacgg cagaaccgca
    11881 acccgggcgc cggccgggag gagttcctcg acgcgcggga cggcggtcat ctgggaggcg
    11941 cagccgccca gcacaagcat caagccggcg agcaggaggc gaaagagtgc cacggcgcta
    12001 cctcgcaatc tgtccgaagg cgccggcgac ttcccggcac accttggtgg tcatctcccc
    12061 cagcgtgtgg gtttcgccga aggagaagat ggggaatttg ggccccccct tgcggctggt
    12121 ctcgtaggac cagacgaccg cgccggtttg gcagtcgaca aagcggacga tgacggcgac
    12181 gggggggaat tcagtctggc cggagcggac catgccgtac tgggagacgg cgccgacgaa
    12241 gattccctcg accccggcgg cctggccgag ctggaaggcc tgttcgctcg tgaggtcttc
    12301 gagggcgttg gcaaagtctc ccttgatcgt ttcggacgcg gccttgttga agtcgccggc
    12361 gttggcgacg ctcacggcgt cgagcatgag cagctcggtg atgaaactcg cagtcacctt
    12421 ctccgcggcg ttctggtcgg aggtgaggtt ggcgaaggtg aggacgccga ctttggagta
    12481 gaagcccatg tccgcttcgc ggttgacgta caggcgtccc acggtgcagc ccgccgccaa
    12541 ggcgaggaag gcagcgccga cgagcagacg gacgaatgtg gaaatagtgc ggttcatatt
    12601 tggagtgtca ccaccgtttg aaacgattca tccacggttt ggagaccgcc ggtattatcg
    12661 gtctcgtttc ggatataggc aagcgagagt gtcgtgcgcg gggacaggta catgatgagc
    12721 tggccgttga cggtcgcggg ccgctggtcg acctcggcca cgccgccggg cagcgaaatc
    12781 gcgcgctccc gactctcgcg ccgccggttg taggaggcgt agacgctgaa cgcccggcgg
    12841 aacgagtagc tcatgtagcc ggtgagggag gtcgtgcggg actcgacggc gccggtgtag
    12901 gtgctgcgaa tcaccgtgag gttcatattc aaattgtcgc gcgggatgta ggtgagcccg
    12961 gcgttgtagt tctgcgagaa ggcggagccc agtttcagca cgccgccctc ggacagcgcc
    13021 gtcaccgtaa accgtccctc aagccggcgg ctgaaatcca cccgggcgtc gagcgaacga
    13081 gtgaactggt agcgccggtt gggatcaacc gggtcgctgt tgcgggtgag cgacaggtcc
    13141 aggtaggtgc ggatgtacgg gaggggcttg aaattcagcg ccgcgttgta ggtgtcgagg
    13201 acgtagtcgc cgtcgtcggc cagccccacc acgtccccct cgccgtcgag cacgctctcg
    13261 gtgcgctcgg actgctggaa gatcgtccgg ttgaaagtga ggcgggtg
//
LOCUS       DAOPOE010000085        13288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5427_length_13288_cov_5.057508, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000085 DAOPOE010000000
VERSION     DAOPOE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5427_length_13288_cov_5.057508"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1753)
                     /locus_tag="PKM94_10740"
     CDS             complement(128..1753)
                     /locus_tag="PKM94_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67150.1"
                     /translation="MIRIVFTATVAAALLGGCASQAIRRETVEVHADPQPVRVEKIPL
                     AAEIERVNPLAYNFFVDGVLFEALGNSYQATESYGKALQYYPDSYVIRASLAENLFRM
                     QRFDDALAVLQPVAPEDAAVWELRGDIYRAGGQIDASVRAYERAVEQDSSRFEIFSFL
                     AGVYSRDGNFPKAKWAFRHLARLDPENHAIWYELGRLHLRLGEADAALTSFRRSAELR
                     ADPTNVMSFIGMGEIYEAREQLDSAKMAFERALAIDTANIVAHRNLAGVYVKLDSLEQ
                     AAVHARIESRLAPLDRNSSRRLGMIYYYIDSLAAADSVFTALVASGENSPVNHEYLGR
                     IALRQDDPPRAVEQFRRALALSDSTWEPWVDLAAAYRAGHQRNEEIAAYREGLGQVRD
                     TAGQAQLMMALGAAYEQTGQFDSAVATFERLVALAPTFDAALNYLGYMLADSGQRLDY
                     ARELIERALQLKPNNAAYLDSYGWVFYRLGRYEEARAQLERAVGLDSDPVMFDHLGDI
                     YHALGQAEAAQHWWHKALELDPDNATIRAKLDL"
     gene            complement(1845..3110)
                     /gene="tyrS"
                     /locus_tag="PKM94_10745"
     CDS             complement(1845..3110)
                     /gene="tyrS"
                     /locus_tag="PKM94_10745"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOD67151.1"
                     /translation="MKTRTDSAAINREFERQWQIITRGAVDVLPEEEFRERVKRSLRE
                     NKPLRVKQGFDPTAPDIHLGHTIGIRKLRQFQDLGHTVVLIVGDYTGMVGDPSGRSAT
                     RPQLDYDTLMKNAETYQRQFFKILDRAKTEVHFNGEWFKQMSFAEVMQLAMKFTVARL
                     LERDDFEKRMRRGIPISVHELFYPLMQAYDSVAIRADVELGATEQKFNLLAGRTIQEA
                     YGVEPQCILTLPVLVGIDGENRMSKSLGNYIGIDESPREIFGKIMKIPDRLIADYAAL
                     VADYTLEELEDIKQKLASPDINPMDVKKMLGERMVEMYHQAGAGAAAREEFGRVFSQK
                     QLPDEIPECPSARIREWGLDPDRIYLVHLMAKAELAKSNSDARKLIQAGAVELDGERV
                     SDPDYEFALPDTGFVVLKVGKRRFLRLRR"
     gene            3299..5452
                     /locus_tag="PKM94_10750"
     CDS             3299..5452
                     /locus_tag="PKM94_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOD67152.1"
                     /translation="MKSPDQKKFRRDRLRTMLVGLVALALLLLAVAAYKTYRVYQSDL
                     PSFEQLHNIEPSLKTKIYDRNGILIQEFFSENRALTPFDQMPPDLVNMLLASEDRKFY
                     DHWGIDVRRVLVVAFSNLLSWDIKAGASTITQQLARMLFLNREQTFDRKFKEALTAVK
                     LERTYSKQEILEMYLNQYYFGSGAYGVAAAAHNFFGKNVAELTISDDAVLIGMLKAPN
                     SNSPFRYPDRALKARNRVLYSYYTYGGLNRAQYDSLKDEPLVVTPPSENVGDAPYFTE
                     EIRQYIFDRYGESMLYSGGLKVFTSLDVGLQRAAEAAVARKVDSLRARIERMYTLGNP
                     DYTELLPDTVDAQGRPIRAYKHVQGAFVAIDNATGDVLALVGGRSFEATKYNRATQGR
                     LQPGSAFKPFVYTAAVDNGFRTSDLIDDLPIVLDIPGASQWRPHNFDDKFMGPITLRD
                     GIRLSRNLVAIRLLLRIKPEQAIFYARRMGITTPLQANPSLALGTSEVIPIELVSAFS
                     VFPNKGIHIPYRMIHKIVDRYGSVIEDNTAIQKEEVLSEQTSYIMVNLMQSVVDAGTG
                     RGVRSRGFTRPAAGKTGTSNNFCDNWFIGFTPQITAGAWVGFDVKTSLGRSQDGAKNG
                     VPIWTEFMMAAHDTLPILDFEEPEGIVHADVCLESGELATDRCTDVRTEIFTADSQPT
                     QRCRLHPSSGRYSAGAAARRPAAPDVDTSEDRTHF"
     gene            5527..5610
                     /locus_tag="PKM94_10755"
     tRNA            5527..5610
                     /locus_tag="PKM94_10755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5561..5563,aa:Leu,seq:caa)
     gene            5696..6595
                     /locus_tag="PKM94_10760"
     CDS             5696..6595
                     /locus_tag="PKM94_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD67153.1"
                     /translation="MIDMPANYLGPALSLLTAIAWACAVILFKKSGETVHPIGLNLFK
                     VLLAALLFVPTIGLSRETFFRSVPAGDYLLVLGSGALGIGIADTIFFFSLNAIGAGRF
                     AVVDCLYSPFVIALSMLFLAERLALPQLAGVAMIISGVVSVTGESHADLPRARLVKGV
                     LLGALAVACTATSLVAVKPLLERSPLLWVTEMRMIGGAAVLLVVLAFHPRRREIVRSV
                     LSVHRWAYTLSGSFMGAYVSTLLWLAGMKLTQASIAAALNQTSNVFMFIFAALFLGER
                     VTPMRTLGIILGVGGAFLVMFGR"
     gene            complement(6665..9952)
                     /locus_tag="PKM94_10765"
     CDS             complement(6665..9952)
                     /locus_tag="PKM94_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD67154.1"
                     /translation="MRSFISALALALFIGAAAQAEEARLLRFPAVHGDNVAFVYAGDI
                     YTAPRAGGAATRLTSHPGLEIFPRFSPDGAKIAFTGQYDGDMAVYVIPVAGGQPQRLT
                     WHPGLQRTSERMGPENVVMGWNRDGSKVLFRSRKESKSDWEGRTYLVDLKGGMPEPLP
                     MASAGFTSLSPDERKAAYCPIYRDFRTWKRYKGGMAQDVWIYDMEKNVSEKITDWEGT
                     DNVPMWYGDRIYFNSDRTGKLNLYRYDLTTGQVTQVTSFTEYDVRWPSLGDDGIAFEN
                     GGYLYVLDLPGEKVAKVSVEVISDRHAMRPEIMEVSDKIGDYDIAPNGKRAVFAARGD
                     IFTVPAKEGNTRALLARSEAREKSVRWSPDGKWIAFYSDISGEEELYLVAHDGSDTVQ
                     LTSGNRMQRNDPEWSPDSRMLAFVDMTEAICCVDAATGRVETIDSAEFGGLGGLAWSP
                     DSRYLAYNKAPGVNRIRTIFIYSFDDRRSYQITPGFTHDYSPVFSADGKYLFFISERN
                     FNPILGSYEFSFVNQAIEDLYLIILQSDGASPFRPGQDEGIAEKTEAGAGGEQEKKGK
                     DRDGGPPPEPVKVRIDIEGIFDRQVAFDLPAGNYGNLAALDGAVLYMSFPIRGLRGPV
                     THDDRTLHKYDLKKKKDYEFARDIGGYRLSADRKCLLVDKDRSYFIVGADGNQADLSE
                     GRLDLSHLSTRVDHVAEYRQMLREVWRGERDFFYDPAMHGVDWDKMWDRYAALLPYAA
                     NRYDLTYVIGEMIGELACSHTYTGGGEYAEIPRGKVGLLGADFAVDKISNRYRIARIF
                     RGENFDDDLRSPLTEPGVNIREGEYLLAIDGRELTADIDPYQLTENSLGQRVTLTVNS
                     RPTRAGARDVQVKPIASEGALRYFAWVEDNRRYVDSVSGGRVGYLHIPDMDSYGLYRF
                     TKMFYYQMRKPGLIIDERWNGGGFVSGLILERLRRTLAAAGKGRYGSNWPDPGDAVHA
                     HMVMLMNEFSCSDGDYFPYFFREYKLGPLMGKRTWGGVIGIDGFNRLIDGGYFTVPGF
                     GIYNLQGEWVMENVGVTPDVEVDNLPERLVAGYDDQLDRAIENVLQRLQEDPKTIPAV
                     KGSPTPR"
     gene            complement(10041..11225)
                     /locus_tag="PKM94_10770"
     CDS             complement(10041..11225)
                     /locus_tag="PKM94_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOD67155.1"
                     /translation="MLTTWLGFGISLAVILAVASRTMPAALLAGAFVLGLTTLPWSVF
                     ADRVTFTLTDPAVLLLAAAMAMVPMIGGAMRQSGQVDALVRNLRVRQRWLLPLSASMM
                     GLLPMPGGALLSAPIVEKAGGGVPDELKAAINNWFRHLFILIYPLSPALIASSKIAGV
                     DVYAAMLHLLPGSLLSVVLGYFFYLRQVNGPTRREGRFSWAELALPLAVVLAAPVLDF
                     TLKRAAGLGNLATVIGTVTAFGLSAGLSRERIDLTALTRRARPWNFALIIIGMFLYVN
                     VFVASDIRELIAALPLPTLILALAAGFGLGFGTGRVDLSSSIIFPIYLAGAGSVTPSV
                     FALIYAAVFFGYMISPVHPCLVVTSEYFKVPVRKIMRRHAAPTAAVLGIVLTIAGVLL
                     AQ"
     gene            complement(11250..11759)
                     /locus_tag="PKM94_10775"
     CDS             complement(11250..11759)
                     /locus_tag="PKM94_10775"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOD67156.1"
                     /translation="MTDKLRPVTVYDIPDNAFKLIADDWMLITAGGPGAFNTMTASWG
                     GVGELWHRKIAICFVRPTRYTYEFMNRHEFFTLTFFDERYREVLNLCGRRSGRDFDKM
                     GGIGLTPIASPRGSVYFAEARLVLECRKVYTHDLDPARFLDPAIEQEYPRRDYHRMYI
                     GQIETCLRR"
     gene            12107..>13288
                     /locus_tag="PKM94_10780"
     CDS             12107..>13288
                     /locus_tag="PKM94_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD67157.1"
                     /translation="MSHPLRFLRRRNLALGALLPALTLLAASSFAQLTAEDIARMQQQ
                     AAREGWTFKVSQNEATQYPLEQLCGLKEPPGWRDSARFVPFDDKGPLRPRFDWRETAG
                     GLPPVRNQGSCGSCWAFGTVGPLECNIKIQDGDIVDLSEQWLVSCNRMGWGCDGGWFA
                     HEYHRWSKDACDSSGAVLEQDYPYTASDAPCACPDPHPYKIRDWAFIDSQSGVPSVGA
                     MKLAIYEHGPISVALAVTSPFHAYSGGVFNACSEGTINHAVTLVGWDDTMGDYGVWIL
                     RNSWGPGWGDNGYMYIAYGCLQVGYGACYVEYYGGVSFSADVQSGWGPLTVNFSGRSG
                     LNLTDWSWDFGDGHTGAGQYTAHTYTTPGSFTVTVSAYDSLGGLRQISKPGFIAALAD
                     TV"
BASE COUNT         2259 a         4603 c         4013 g         2413 t
ORIGIN      
        1 ttgggagggg tgacggccag gagcgcgggc ggcgtgcggt cgacgtcgcc gcccggcggc
       61 ggagcgatct cggcgcagcc cgccaggagg acggccaggg ccgcggcccg gaggaccgca
      121 gcgatcgtca cagatccagt ttcgctcgaa tggtcgcgtt gtcgggatcg agttcgagtg
      181 ctttgtgcca ccagtgctgc gctgcctccg cttggccgag agcatgatag atatcgccca
      241 gatgatcgaa cattaccggg tcgctgtcaa gtcctaccgc gcgctcgagc tgcgcgcgcg
      301 cctcttcgta acggccgagc cggtagaaca cccatccgta gctgtccaga taggcggcgt
      361 tgttgggctt gagttggagc gcccgctcga tcagttcccg tgcgtaatcc aaccgctgcc
      421 cggagtcggc cagcatgtac cccaggtagt tcagggcggc gtcgaacgtc ggggccagcg
      481 ccaccaggcg ctcgaaggtg gcgaccgcgc tgtcgaactg gccggtctgt tcgtaggccg
      541 cccccagcgc catcatcaac tgggcctgcc cggcggtgtc ccgcacctgc cccaatccct
      601 cgcggtaggc ggcgatctcc tcgttgcgct gatggccggc ccggtaggcg gcggccagat
      661 caacccatgg ttcccaggtc gaatccgaca gagcgagcgc ccggcggaac tgctcgaccg
      721 cccgcggcgg atcatcctgg cgcagcgcga tccgccccag gtactcgtgg ttgaccgggc
      781 tgttctcgcc gctcgccacc agcgccgtaa ataccgagtc ggccgcggcc agggagtcta
      841 tatagtagta gatcatcccc agccgccggc tggaattccg gtccagcggg gccagccgac
      901 tctcgatacg ggcatgcacg gccgcctgct ccagggagtc gagcttcaca taaacgccgg
      961 ccaaattccg atgggcgaca atgttggccg tgtcgatcgc gagcgcccgc tcgaacgcca
     1021 ttttggcgga atcgagctgc tcccgtgctt catatatttc tcccatcccg ataaacgaca
     1081 tcacgttcgt ggggtccgca cgcaactccg ccgagcgtcg gaaagacgtc agcgccgcat
     1141 ccgcctcccc cagccgcaag tgcagccgcc ccagttcgta ccagatcgcg tgattttccg
     1201 gatcgagccg ggccagatgg cggaacgccc acttcgcttt cgggaaattc ccgtcccgcg
     1261 aatagacgcc ggccagaaac gagaagatct cgaagcggct ggaatcctgc tcgacggccc
     1321 gctcgtaggc acgcaccgag gcgtcgatct gcccccccgc gcgatagatg tcgccgcgca
     1381 attcccacac cgcggcgtct tccggcgcaa ccggctgcag caccgccagc gcatcgtcga
     1441 aacgctgcat gcgaaagagg ttctcagcca gcgaggcgcg gatcacatac gagtccgggt
     1501 agtactggag ggctttgccg taactctcgg tcgcctggta cgaattgccg agggcctcga
     1561 acagcacgcc gtcgacaaaa aagttgtaag caagcgggtt gacccgctcg atctccgccg
     1621 ccagcggaat tttctccacc cgcaccggct gcgggtcggc atgcacctcg accgtctcgc
     1681 ggcgaatcgc ctgcgaggcg cagccgccga gaagggccgc agccaccgtt gctgtgaaaa
     1741 ctatccttat catcttgaac cgtctccggg ggaccggacg ccgcccccgg ggagagcgaa
     1801 aggtcaccct tactacaacg tccgccgtag aaagtgcccg gtcgctatcg ccgcagcctg
     1861 aggaaccggc gtttgcctac tttcagcacg acgaagccgg tgtcgggcag cgcgaactcg
     1921 tagtcgggat ccgacacccg ctccccgtcg agctcgaccg ctccggcctg tatcagtttg
     1981 cgggcgtcgc tgttcgactt cgccaactcg gccttggcca tcaggtgcac cagatagatt
     2041 cggtcgggat caagtcccca ctccctgatg cgggccgagg gacattcggg aatctcatcg
     2101 ggcaactgct tctgggagaa gacgcgcccg aactcctccc gggccgccgc gccggctccg
     2161 gcctggtggt acatctcgac catgcgttcc ccaagcatct ttttgacatc catcggatta
     2221 atatcgggac ttgcaagttt ttgtttaatg tcctcgagtt cctcaagggt gtagtcggcc
     2281 accagcgcgg cgtagtcggc gatcagccgg tcgggaatct tcatgatctt gccgaaaatc
     2341 tcccgcggcg actcgtcgat tccgatatag tttcccagcg acttggacat ccggttctcg
     2401 ccgtcgatcc ccaccagcac cggcagggtg aggatgcact gcggctcgac gccgtaagcc
     2461 tcctggatcg tccttccggc cagcaggttg aacttctgct cggtcgcgcc gagctcgaca
     2521 tcggcccgga tcgccaccga atcatacgcc tgcatgagcg ggtagaagag ctcgtggacc
     2581 gagatcggaa tcccgcgccg cattcgcttt tcgaaatcgt cccgctccaa gagccgggcc
     2641 accgtgaact tcatggccag ctgcatcacc tcggcgaacg acatctgctt gaaccactcg
     2701 ccgttgaaat gcacctcggt tttcgcgcgg tcgagaatct tgaaaaactg ccgctggtaa
     2761 gtctccgcgt tcttcatcag ggtatcgtag tccaactgcg gccgggtggc cgaccgcccc
     2821 gaggggtcgc cgaccatgcc ggtgtagtcg ccgacgatga gcacgacggt gtgtccgagg
     2881 tcctgaaact gccgcagctt gcggatgccg atggtgtggc cgaggtgaat gtcgggcgcc
     2941 gtcgggtcaa acccctgctt cacccgcagc ggcttgttct cgcggagcga ccgcttcacc
     3001 cgctcccgga actcctcctc gggaagcacg tcgaccgccc cgcgggtgat gatctgccac
     3061 tgccgttcga actcccggtt gatcgccgca ctgtcagtcc gcgtcttcaa ctcgctctcc
     3121 ttggtgtgca tcttttgttc acgtatcgac atataatcgg aaaagcctca gcggcggccg
     3181 cctggtcgga aggctgctgc cgcgtggtga acaataaacg gccgaggctc cggcggaaca
     3241 agcgggattt tgttttagct tgcgtgtccg ggagaccggc ctgttattga cagacaggat
     3301 gaaatcgccc gatcaaaaga agttccgccg cgatcgcctc cgcaccatgc tggtcggcct
     3361 ggttgccctg gccctcctgc tcttggcggt cgccgcctac aagacctacc gcgtctacca
     3421 atccgatctc ccctcgttcg agcagctcca caacatcgaa ccgagcctga aaaccaagat
     3481 ctatgaccgc aacggaatcc tcattcagga gttcttcagt gaaaaccgcg ccctgacccc
     3541 ctttgaccag atgcctcccg acctggtcaa catgctcctg gcctcggagg accggaagtt
     3601 ctatgaccac tgggggatcg atgtgcgccg cgtcctcgtg gtcgccttct ccaacctcct
     3661 gagctgggac atcaaggccg gcgcctcgac catcacccag cagctcgccc ggatgctctt
     3721 cctcaaccgc gagcagacgt tcgaccgcaa gttcaaggag gccctcaccg ccgtcaaact
     3781 cgagcggacc tattccaagc aggaaatcct cgagatgtac ctgaaccagt actatttcgg
     3841 cagcggcgcc tacggtgtcg ccgccgccgc ccacaatttc ttcggaaaga acgtggcgga
     3901 gctgacgatc agcgacgacg cggtgctgat cggcatgctc aaggctccca acagcaactc
     3961 ccccttccgg tatcccgacc gggccctcaa agcccgcaac cgcgtcctct attcctacta
     4021 cacgtacggc ggattgaacc gggcccagta cgattcgctc aaggacgagc cgctcgttgt
     4081 caccccgccc tcggaaaacg tcggcgacgc cccctatttc accgaggaaa tccgccagta
     4141 catctttgac cgctacggtg agtcgatgct ctattccggg gggctgaagg tgttcacgtc
     4201 gctcgacgtc ggtctgcagc gcgcggccga ggcggccgtg gcccgaaagg tggattcgct
     4261 ccgcgcccgg atcgaacgga tgtatacgct gggcaatccc gactacaccg agctgcttcc
     4321 cgacacggtc gacgcccagg ggcgccccat ccgcgcctac aagcacgtgc agggggcctt
     4381 tgtcgccatc gacaacgcca ccggcgacgt tctcgcgctg gtgggcgggc ggtcgttcga
     4441 ggcgaccaaa tacaaccggg ccacgcaggg ccggcttcag cccggctcgg ccttcaaacc
     4501 gttcgtctac acggcggccg tcgacaacgg tttccgcacc tccgacctga tcgacgatct
     4561 ccccatcgtc ctggacatcc ccggcgccag ccagtggcgg ccgcacaatt tcgatgacaa
     4621 gttcatgggg ccgatcacgc tccgcgacgg catccggctc tcccgcaacc tcgtcgctat
     4681 ccgcctcctt ctccggatca aaccggagca ggccatcttc tacgcccgcc ggatgggcat
     4741 caccaccccg ctccaggcga acccctccct cgccctcggc acgagcgagg tgattcccat
     4801 cgagctggtg tccgcctttt cggtctttcc gaacaagggg atccacatcc cctaccggat
     4861 gatccacaag attgtcgacc gctacggcag cgtcatcgag gacaacacgg cgatccagaa
     4921 ggaggaagtg ctttcggagc agacctccta tataatggtg aacttgatgc agtcggtggt
     4981 cgacgcgggc acggggcgcg gggtccgttc gcgcggtttc acccgcccgg ccgcgggcaa
     5041 gaccgggacc tcgaacaatt tctgcgacaa ctggttcatc gggttcacgc cccagatcac
     5101 ggccggcgcc tgggtcggtt tcgacgtcaa aacctcgctt ggccgcagcc aggacggcgc
     5161 caagaacggc gttccgatct ggacggagtt catgatggcc gcccacgaca ccctccccat
     5221 ccttgatttc gaggagccgg aggggatcgt acacgccgac gtctgcctcg aatccggcga
     5281 gctggccacc gaccgctgca ccgacgtgcg gaccgagata ttcacggccg acagccagcc
     5341 cacgcagcgc tgccgcctgc acccgtcatc cggccgctac tccgcaggcg cggcggcccg
     5401 ccgcccggcc gcgccggatg tcgacacgag cgaggaccgg acacatttct gacagttgcg
     5461 cgcccggccg cgcaccgatt ccatcttgac agaccggcgc ccgcgccgga tattggctca
     5521 cgctctgccg gagtggtgaa actggtagac gcaggggact caaaatcccc cggtggcaac
     5581 accatgccgg ttcgagtccg gcctccggca tttctcgcag cgcacggttc tcgacggcgg
     5641 caggagcgcc tttcccgctt gtccccggtt cttccgtcgg ttacctttga ctcccatgat
     5701 cgacatgccg gccaactatc tcggtcccgc cctctcgctt ctcaccgcga tcgcctgggc
     5761 ctgcgccgtc atcctgttca agaagagcgg cgagaccgtc caccccatcg gactcaatct
     5821 gttcaaagtc ctcctggccg ccctcctgtt cgtccccacc atcgggctct cccgggagac
     5881 cttctttcgc tccgttccgg cgggggatta cctgctggtg ctcggctccg gggcactcgg
     5941 catcgggatc gccgacacga tcttcttctt cagcctcaac gccatcggcg ccggccgttt
     6001 cgccgtcgtc gactgcctct acagtccctt cgtcatcgcc ctgtccatgc tctttctcgc
     6061 cgagcgtctg gcgctgccgc agctcgccgg cgtcgccatg atcatttccg gggtcgtcag
     6121 cgtgacgggg gagtcgcacg ccgatcttcc ccgcgcccgt ctcgtgaaag gagtgcttct
     6181 gggggccttg gcggtggcct gcacggccac cagcctggtc gcggtcaagc cgctgctgga
     6241 gcgctcgccg ttgttgtggg tcaccgagat gcgcatgatc ggcggtgcgg ccgtgttgct
     6301 ggtcgtcctg gctttccacc cgcgccgccg cgagatcgtc cgttccgtgc tctccgtcca
     6361 ccgctgggcg tacacgttat cgggctcgtt catgggcgct tacgtctcca ccctcctctg
     6421 gctggccggc atgaaactga cgcaggcctc gatcgccgcc gccctcaatc agacgtccaa
     6481 cgtcttcatg ttcatctttg ccgccctctt cctcggcgag cgagtgaccc cgatgcgcac
     6541 gctcggcatc atcctcggcg tgggcggcgc gtttctcgtc atgttcggcc ggtgaccgcg
     6601 cttcaccgtt gcggccgctg ccccgccggg cagcggccgc ggatacggcg ggagttggtc
     6661 gctttcagcg cggcgtcggc gaacccttga ccgccgggat cgtcttggga tcctcttgca
     6721 gccgctggag aacattctcg atcgcccggt cgagctgatc gtcgtacccc gcgaccagcc
     6781 gctcggggag gttgtcgacc tcgacatccg gggtcacccc gacattctcc atcacccact
     6841 ctccctggag attgtagatg ccgaacccgg gcaccgtgaa gtagccgccg tcgatcagac
     6901 ggttgaatcc gtcaatcccg atcacgcctc cccaggtccg cttgcccatg agcgggccga
     6961 gtttgtactc gcggaagaaa tacgggaagt agtccccgtc ggagcaggag aattcattca
     7021 tgagcatgac catgtgggcg tgcaccgcgt cgcccggatc gggccagttg ctgccgtagc
     7081 ggcctttgcc cgccgccgcc agcgtccggc ggagccgctc gagaatcaac cccgagacga
     7141 acccgccgcc gttccagcgc tcgtcgataa tcagccccgg cttgcgcatc tggtagtaga
     7201 acatcttcgt gaaccggtac agcccgtagg aatccatgtc gggaatgtgc aggtatccga
     7261 cccggccgcc ggagaccgaa tcgacgtacc gacggttgtc ttcgacccag gcgaagtagc
     7321 gcagcgcgcc ctccgatgcg atcggtttca cctgcacgtc gcgggccccc gcgcgcgtcg
     7381 gacggctgtt gaccgtcagc gtgacccgct ggcccaggct gttctcggtc agctggtagg
     7441 ggtcgatgtc cgccgtcagt tcccggccgt cgatcgccaa aagatactcc ccttcccgga
     7501 tgttgacccc cggttccgtc agcggtgagc gcaggtcgtc gtcgaaattc tccccgcgga
     7561 agatccgcgc gatccggtag cggttcgaga ttttgtcgac ggcgaaatcc gcacccagca
     7621 gcccgacttt cccgcgcggg atctccgcat actctccgcc ccccgtgtag gtgtgcgaac
     7681 aggccagctc gccgatcatc tcgccgatca cataggtcag gtcatagcgg ttcgccgcat
     7741 agggcaggag ggcggcgtag cggtcccaca tcttgtccca gtcgacgccg tgcatggcgg
     7801 ggtcgtagaa gaaatcgcgc tctccgcgcc agacttcgcg gagcatctgg cgatactcgg
     7861 cgacgtggtc cacccgggtg ctcaggtgcg acagatcgag ccgcccttcc gacaggtcgg
     7921 cctggttgcc gtcagccccg acgatgaaat acgaccggtc cttgtcgacc agcaggcact
     7981 tgcggtcggc gctcaggcgg tacccgccga tatccctggc gaattcatag tccttcttct
     8041 tcttcaggtc gtacttgtgg agcgtccgat cgtcgtgggt gaccggaccg cgcagcccgc
     8101 ggatggggaa ggacatgtac agcaccgcgc cgtcgagggc cgccaggttg ccgtagttgc
     8161 cggccgggag gtcgaacgcg acctgccggt cgaagattcc ctcgatgtcg atccggacct
     8221 tcaccggctc cggcggcggc ccgccgtccc ggtctttccc cttcttctcc tgctcgcctc
     8281 ccgcgcccgc ctcggtcttc tccgcgattc cctcgtcctg tcccggccgg aacggcgacg
     8341 cgccgtcgct ctggagaatg atcaggtaca gatcctcgat cgcctggttg acgaatgaga
     8401 actcgtacga cccgaggatc gggttgaagt tccgctcgga gatgaagaag agatacttgc
     8461 cgtccgctga aaacacgggc gagtagtcgt gggtgaagcc gggcgtgatc tggtaactgc
     8521 gccggtcgtc aaaggagtag atgaagatcg tgcggatccg attcacgccg ggcgccttgt
     8581 tgtaggcgag gtagcggctg tcgggggacc aggcgaggcc gccgagtccg ccgaactcgg
     8641 ccgagtcgat cgtctccacg cggccggtgg cggcgtcgac acagcagatc gcttcggtca
     8701 tatcgacgaa agcgagcatg cggctgtcgg gcgaccactc gggatcgttc cgctgcatcc
     8761 ggttgccgct ggtgagttgc acggtgtcgc ttccgtcgtg cgccaccaga tacagttctt
     8821 cctcgccgct gatgtccgag tagaaggcaa tccacttgcc gtcgggcgac caccggaccg
     8881 atttctcccg cgcctccgag cgggccagga gcgcccgggt gtttccctcc ttggcgggca
     8941 cggtgaagat gtccccgcgg gccgcgaaca ccgcccgctt gccgttcggt gcgatatcgt
     9001 aatcgccgat cttgtccgac acctccatga tctccggccg catcgcgtgc cggtcggaga
     9061 tcacctccac cgataccttg gctaccttct cccccggcag gtcgagcacg tagagatagc
     9121 cgccgttctc gaatgcaatc ccgtcgtcgc cgaggctcgg ccaccggaca tcgtactcgg
     9181 tgaacgacgt cacctgcgtg acctgcccgg tggtcaggtc gtagcggtac aggttgagtt
     9241 tgccggtgcg gtcgctgttg aagtaaatcc ggtcgccgta ccacatcggc acgttgtccg
     9301 taccttccca gtcggtgatc ttctccgaga cattcttctc catgtcatag atccacacgt
     9361 cctgggccat tccccccttg tagcgtttcc aggtgcggaa atcccggtag atggggcagt
     9421 aggccgcttt gcgttcgtcc ggcgacaggc tggtgaaccc cgccgacgcc atcggcagcg
     9481 gttcgggcat tccccctttc agatcgacca ggtaagtcct cccctcccag tcgcttttcg
     9541 attctttccg cgatcggaac agcaccttcg atccgtcgcg gttccagccc atgaccacgt
     9601 tctccggccc catgcgttcg ctcgtgcgct ggagcccggg gtgccaggtc agccgctgcg
     9661 gctggccgcc cgcgaccggg atcacgtaca cggccatgtc gccgtcatac tgcccggtga
     9721 aggcaatctt cgcgccgtcc ggcgaaaacc ggggaaagat ttccagtccg ggatggctgg
     9781 tcagccgcgt ggccgccccg ccggcccgcg gcgccgtgta gatgtccccg gcgtagacaa
     9841 acgccacgtt gtcgccgtgc actgccggaa accgcagcag ccgggcttcc tccgcctgtg
     9901 ccgccgcgcc gatgaagagc gcgagcgcca gagcactgat gaatgatcgc attgaacctc
     9961 cggaaagtac gaaccgcttt agccatttat gacgaaaaga ggcacgccaa ggttgcctct
    10021 tgatcttacc tctttggccg tcactgcgcc agcagcacac cggcgatcgt cagcacaatc
    10081 cccagcacgg cggccgtggg cgcggcgtgc cgccgcataa tcttccgcac cggcactttg
    10141 aaatactcgc tggtgaccac caggcagggg tggaccggcg agatcatgta tccgaaaaac
    10201 acggccgcgt aaatcagggc aaacactgag ggcgtcaccg accccgcgcc ggccaggtag
    10261 atggggaaga tgatcgaaga cgacaggtcc acccggcccg tgccgaaacc gagcccgaat
    10321 ccggcggcca gcgcgagaat cagcgtcggc agcgggagcg ccgcgatcaa ctcccgaatg
    10381 tccgaggcca cgaagacgtt tacgtagagg aacatgccga tgattatgag ggcaaaattc
    10441 cagggccgcg cccgccgcgt caacgccgtc agatcgatcc gctcccggct cagtcccgcc
    10501 gacagcccga acgccgtcac cgtgccgatc acggtcgcca ggttcccgag gccggccgcc
    10561 cgcttgagcg tgaaatcgag caccggggcc gcgagcacca cggccagcgg cagcgccagt
    10621 tccgcccacg agaaccgccc ctctcggcgc gtcggcccgt tcacctggcg gaggtagaag
    10681 aagtacccca gcaccaccga cagcagcgag cccggcagca ggtgcagcat ggccgcgtag
    10741 acatcgacgc cggcgatctt cgacgaggcg atcagggccg ggctgagcgg atagatcaga
    10801 atgaagaggt ggcggaacca gttgttgatc gccgccttca actcgtcggg gacgccgccg
    10861 cccgcttttt ccacgatcgg cgccgagagc agcgcgcctc ccggcatcgg cagaagcccc
    10921 atcatcgagg ccgacagcgg cagcagccag cgctggcgca cgcgcaggtt gcgcacgagc
    10981 gcgtccacct gcccgctctg ccgcatcgcc ccgccgatca tcggcaccat ggccatcgcc
    11041 gccgccaaga ggagcaccgc cggatcggtg agggtgaagg ttacgcggtc ggcgaacacg
    11101 ctccacggca gggtggtcag gccgagcacg aaggccccgg ccagcaaggc cgccggcatg
    11161 gtgcggctgg cgaccgccag aatgaccgcc agcgagattc cgaaacccag ccacgtcgtc
    11221 agcatccggc tctcacccgc tccctgctct caccgccgga ggcacgtttc gatctgcccg
    11281 atgtacatgc ggtggtaatc ccgccgcgga tattcctgct cgatcgccgg atccagaaag
    11341 cgcgccgggt cgagatcgtg cgtgtagact ttgcggcact cgagcacgag gcgcgcctcc
    11401 gcaaagtaga ccgacccgcg cggcgaggcg attggcgtca ggccgatacc gcccattttg
    11461 tcaaaatccc gccccgaccg gcggccgcag aggttgagca cttcccggta gcgctcgtca
    11521 aaaaacgtca gagtgaaaaa ctcgtgccgg ttcatgaact catacgtgta gcgcgtcggc
    11581 cggacgaaac agatggcgat cttccgatgc catagctccc ccaccccgcc ccacgaggcg
    11641 gtcatcgtgt tgaacgcccc cggcccgccg gccgtgatga gcatccagtc gtcggcgatc
    11701 agtttgaagg cattgtcggg aatgtcatag actgtgacgg gtcgaagttt gtcggtcata
    11761 gcggtacctt cctcaccccg ccggtccgca gcctgcggga tcacacacca gcgcggaaag
    11821 taccgtgcgg acggtcccaa gacaagccct ttctcgcgcc gccgccggct gtgggcggac
    11881 ccggcgctgt tcgatgcggc ggtggaacgg tcggctacgc tgccgccagg aactggccgc
    11941 gccccacggg accgcttttt tgtttacaaa ggcccggttt caggtatatt aagaatcaat
    12001 aacggtcccc tcgcgccgcc gccgctgatc cgccgccctc cgggggaggc gtcctgacga
    12061 tcagccggtg tggccggctc gcgcgaactg cgaaaggacg acagtgatgt cacacccgtt
    12121 gcgtttcctc cgccggcgga atctcgccct tggcgctctc cttccggctc tcacccttct
    12181 tgctgcctcc tcgttcgctc agctcacggc cgaggacatc gcccgcatgc agcaacaggc
    12241 ggcccgggag ggctggactt tcaaggtctc ccagaatgag gccacccagt atcccctcga
    12301 gcagctctgc ggccttaaag agccccccgg ttggcgcgac tccgcgcggt tcgtcccctt
    12361 cgacgacaag gggccgctcc gcccccgctt tgactggcgc gagacggccg gcggcctgcc
    12421 gccggtgcgc aaccagggca gctgcggcag ttgctgggct ttcgggaccg tcgggcccct
    12481 cgagtgcaac atcaagattc aagacggcga catcgtggac ttgtccgagc agtggctcgt
    12541 gagctgcaac cgcatgggct ggggctgcga cggcggctgg ttcgcccacg aataccatcg
    12601 ctggtccaag gatgcctgcg acagctccgg cgccgtcctc gaacaggact acccgtacac
    12661 ggcctccgac gctccctgcg cctgtccgga ccctcacccc tacaagatcc gcgactgggc
    12721 cttcattgac tcccagtccg gcgtcccgag cgtcggcgcc atgaaactgg ccatctacga
    12781 gcacggtccc atttccgtgg ccctcgccgt cacttccccc tttcacgcct attcgggcgg
    12841 cgtgttcaac gcctgttccg aaggcaccat caaccacgcc gtgacgctcg tcggatggga
    12901 tgacaccatg ggcgactatg gggtctggat tctgcgcaat tcgtggggac cgggctgggg
    12961 ggacaacggc tatatgtaca tcgcctacgg ctgtctccag gtcgggtacg gcgcgtgtta
    13021 tgtcgagtac tacggcgggg tgtccttctc ggccgatgtc cagtcgggct gggggccgct
    13081 gacggtcaac ttctccggcc gctccggcct caacctgacc gactggtcct gggatttcgg
    13141 cgacggccac accggcgccg gccagtacac cgcccacaca tacacgactc cggggagctt
    13201 caccgtgacc gtctccgcgt atgattctct cggcggcctg cggcagatct ccaagcccgg
    13261 cttcatcgcc gctctcgccg acaccgtg
//
LOCUS       DAOPOE010000086        13137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5514_length_13137_cov_5.813561, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000086 DAOPOE010000000
VERSION     DAOPOE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5514_length_13137_cov_5.813561"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PKM94_10785"
     CDS             <1..947
                     /locus_tag="PKM94_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD67158.1"
                     /translation="KLAARIAASDSTVLIRGESGTGKDLIARAIHRHSSRAKGPFVTI
                     NCAALPESLLESELFGHKKGSFTGAVKDKDGLFKVADGGTFFLDEVGNTSMAIQVKLL
                     RVLEDKKITPVGDTRPLEVDVRLIAATNADLEAEVKAARFRADLFYRLNVIPIAIPAL
                     RERADDIDLLAQHFIDRCCAKLGVPVKRLTDEARLLLRNYPWPGNVRELENTIERAAL
                     LSRSETLDVGDFPDKLREPSAAPAGLIQPDQPATPTLESIEKAYIHYVMSQTEGRKAE
                     AARILGIDASTLYRKIQRYNLTDFVTRKDKPSGTPDGA"
     gene            1027..1455
                     /locus_tag="PKM94_10790"
     CDS             1027..1455
                     /locus_tag="PKM94_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67159.1"
                     /translation="MNRIRNNRGFTLIELMIVVVIIGILAALAIPRYMTASAKSKQSE
                     AKAILKQVYEGERAYFHASENNTYWTPAAPASAGNRTAFAELCVEIGASARYTYTITT
                     AAGSFTATATANIDDDAVLDTWTINEQGVLTNTVNDILVS"
     gene            1812..2207
                     /locus_tag="PKM94_10795"
     CDS             1812..2207
                     /locus_tag="PKM94_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD67160.1"
                     /translation="MFSTIRNRKGFTLIELMIVVVIIGILAALAIPRFMQASMKSKQS
                     EAKGILKQIYTMEHAYRQEHDVYVTDLNTIGVELQPQPPRRYGYVIALNGTGFTATAT
                     ANLDDDATIDTWTIDDGGILQCTVNDVTG"
     gene            2345..4297
                     /locus_tag="PKM94_10800"
     CDS             2345..4297
                     /locus_tag="PKM94_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67161.1"
                     /translation="MTNLRFVPLALAVLCLPAAGAPGEPPPEIWGLTHLADYLALTPA
                     DISFRPDYTEPDSFRLQVLSGLMENPLGMIDYAAGLRRAYTKGQPEVTAALLSRDLAV
                     VGRPDRTPPYRPTGPEMQQGYNLFYASPGLNQLMARAGLYLDVIFPRSAETALASLSP
                     AQRRFLTAEFKELLVSHAEEEFFTPEQLDSVEKIEEKYTEEFTAFGWRIDKDPILTAG
                     FDCLRDLMPEIAALRRDLAAGTLDAGAVMKGLGQIPEKGVGEESYLGRQPGWKVGGPG
                     PDYYEGDYWFIFDFGGDDVYRLSYDPARPHGVIIIDLAGDDRYRGLTDFALASGCLSA
                     GLLLDFGGNDRYDAQSFALGSGYFGFGLLYDAAGDDRYDGDTHVMAAGSFGLGLLIDE
                     GGRDCYNAALYAQGFGFTEGMGVIYDTDGSDSYYAGGKYKDILRYEDHYLSLSQGFGY
                     GLRPWTSGGIGAIIDDRGSDAYYTDIFGQGTSYWWSLGVIYDSSGNDTYNSFQYAQGC
                     ATHMTLGVLIDDAGNDAYIGKGLMQGVGHDYSCGLLLDRAGDDLYTAWDLSQAAGSAN
                     GVGILIDNAGADRYFIRNADNTHGYGNPRRDFGSIGLFLDLGGEDLYNGYGGDNLYWR
                     TPSQWGGGMDIERAPRPDSAVIGEVQ"
     gene            4297..5355
                     /locus_tag="PKM94_10805"
     CDS             4297..5355
                     /locus_tag="PKM94_10805"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD67162.1"
                     /translation="MFSDFKNQDTLQKPRTASDGRASHADPAARRPLWRPGGRVRTLI
                     GGLLLVIVLAPALSAQIDLQHRIDSLFVIASSGEVRYQKLTGPAMDSIAAFGQPAVPY
                     LIDKFDTKSARDRWTIIWILQRIGSPAVPDLVRALDRADPLVVSRVAWALGDIKDTGA
                     VLPLIAKSTHAAWQVREETIGSLGDIGDRRGDNAILAALGDSIGQVRKAAAVAAGRLK
                     ILEAAAPLVHRFGDPFYGARLAAVDALLQLDTVLSLPVLIDSLASADAFVSALSAAAL
                     GRIGADRALDALFRQATEDPDPARRVPAACALIEADPLDRCRYREKLLAAQPDRLGRL
                     KVESALKTARHVEKGTAQ"
     gene            5330..6859
                     /locus_tag="PKM94_10810"
     CDS             5330..6859
                     /locus_tag="PKM94_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOD67163.1"
                     /translation="MSKKELHSKLDRLRGHIVAKPAAPPPEVLPERCRVLAERLDGEA
                     VCTARGGFVLIRKRFPSVYTHGEIALADFAARTDFPASAFTASDDPARLPVADLRFFD
                     LETIGLGGAGTAAFLVGLGALVEGGFEVRQYLLPDYDDEAALLAAVAEEFSDRTVLVS
                     YNGAAFDLPILLDRFTINRVGREIPRARHVDLLHAVRRLFRRRLRQCDLGNIERDVFG
                     FRRFDDIPGYLVPSVYFDWLSDHKLDEMEKVLQHNRHDIVSLYFLAAVLAEVFESRGE
                     RLSEIDDLHSLARVFNRRRDFDLVVDLNRRVESLAGTPDPLADHPDPRADRAQPGGHP
                     GARALPADVLFFHAQTHKRRGEYDEAVVIWKQLASSIPAPPATGPATGPASPPATGPA
                     PPPATGPATGSSHLSFPRRREPRHTPTGASDLPIRHNLPADQTPSGPGLTSPADAGTL
                     LRVAYRACLELAIHYEHRAKEFALALAYTRRAESLPDLNPSRRRSLQKRRDRLVRKQE
                     A"
     gene            6984..9542
                     /locus_tag="PKM94_10815"
     CDS             6984..9542
                     /locus_tag="PKM94_10815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD67164.1"
                     /translation="MSDFAEDDVTRSIAPLAEGQIVGHFRIESRIGAGGMGTVFLAHD
                     MDLDRKVALKHLSPELAQDEQFQARFRREARILANLNHPNIVGIYEVFQAGNRSFIAM
                     EYVDGRPLSAQPDRPLAEICQTAVEIASGLKTAHDAGIVHRDIKPANILVGQDGRVKI
                     LDFGLAKRTDDQDITRTGGTAGTFAYMSPEQVRGTAVDRRSDIFSFGVVLYEMVLGRR
                     PFRGEYAAEIAHRIVAEPPSALDPAGTGIPPELLRIIGRCLEKDPVDRYQETGQVVDD
                     LKHFQRVLSGSGDPFAVISSPRSGVGAAPVADALDASQIPARPPQPKPLFLLFGLGLA
                     AVGVALLLAFTPQPLSELERATTAPAQVEKAALDLARRDHSGLLGFRSYTVSDADAEL
                     QEIREFLRPSDREWEALSQWRPAQFYRTAAVSADQQERYDVLTTLDGRCFSFSHSLRP
                     DRRSDSASVDSLRSAAGQYAQAFLGRDLDSLREMPVAVSFSAGVAQTSFQWVEPDTLL
                     AGAVGEVRATFSGAALTDIRSTAAFPEVLRRTIASRGPDWISTGTFCAVTLAFLAACI
                     FVTKKRMWQFPSPSVLVAAYALMVIGLLGGTGAYEKFADTGTAGVLTHVLLLLVLFAF
                     LAVAVYLVIAAVYGVVGRVKPGLLAGLAPLLKGRMERPALAAAASTGFVCGAALYVVC
                     MLYRHSAAVLFDTAGSLSSSSEYVNSGVQVLSLTILGLPFTCAAVGLFVLLAEILEHY
                     TPVGKRAWLLLTLVVTVLVAANQTSAAGDSAVDLPALWLVIFIFVSVWLTNRYGLFAG
                     ALAVVVYNLVDSAVMLAHYGHGGFLKDSLLCLLLWGVPAVYATYYAVRRPAPAK"
     gene            9704..11041
                     /locus_tag="PKM94_10820"
     CDS             9704..11041
                     /locus_tag="PKM94_10820"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD67165.1"
                     /translation="MTDAKTPAMTEQDLFQIILRDHEELASLPQSLAEVLRLARDPDS
                     SARDLAAVLMRDPGLTTKLLRLANSPYFGAGRRISSVTQAVMTLGMRAVTALALSASV
                     YDLTGKWAGAVDRLRFWRHSLAVAIGARSIAESIRYECSEEAFVAGLLHDIGLLVLEK
                     SFPERYARLWRQAEAGESLPDLEDQVWGTNHARVGQFLLEQWHIPEAVCSAVGKHHTE
                     YPADDHDPAFRLPQIVALANDIAQFTIVKARPLHAQHMDRQDILCGNLKIDADRLQKI
                     REDLLSRTMQEAAFLEIEIGSNEVLLTEANRILYHQFIALERLLRENRAMQREIARGQ
                     LEKSALETLKTITATFNHYINNAVGAILGRAQLVQHDIRRGTFARNPESALESMEIIV
                     NGVKTIRSVMEELTNLSAFKTTVYYDDTYIIDIEKKIRKKVEAIGDSPAPAAK"
     gene            complement(11114..>13137)
                     /locus_tag="PKM94_10825"
     CDS             complement(11114..>13137)
                     /locus_tag="PKM94_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67166.1"
                     /translation="GARRGRRAGAATAVAVMCVLMGGFSPAGPVWAQAPLPPPPPAGD
                     RHQDRAEDARREELQRLSREVRDLGREYLELLNELKEVMKDYNRYLAEAAVKSRSAGR
                     SGQTAASTVTAEQKELQVLMERLAAGAYLKNALQLEGDLSRVRAALDSQREAVRADRR
                     AYALSENLGRSLEAITIQLESELADLPSENDGLPPELYFCFQQASDSGQVDAEKLRQV
                     YVRLLGRDGNVQVAVPDFDFTFDFDPAQLAKLEYLDSLFGDSMCAVIPQVYVASAKPP
                     APPRTPVVVGDRNIVFTGAGGKTEVVREYGDSLRVASRAPITITNPNGDLRLIGWDRE
                     LLLVRGEITLGADSRKAAEALAEEVRLRLERSGSGYEVGYLMPELRDTRQKAANRTLT
                     VMVPQDNPLIVTSPFGSVVLESLENDVMLTTEHADVTGSDIVGRVGLTCQMGSVRLTG
                     VEGHIAITARRTPVELQGCDGEVTIDNEYASVQLAECAGPVDIRNSGQVRISYHQGDI
                     SVDNSNGQVEVQHSEGNCRLTSRYQPMQIAYVKGSVTAENSHAPIVISDITGRIEAGN
                     SFAPIQATDFSGPISLTNSNGPITVSVSGPLQGPSAIRSTGGVINLTLEPGIDLLMRA
                     TAVDGKIESSYPLSLKQAGTTTVAELALGKARPELTITGTRTRIVVGED"
BASE COUNT         2110 a         4851 c         3857 g         2319 t
ORIGIN      
        1 agaagcttgc cgcccgtatc gccgcctccg actccaccgt tctcatccgc ggcgaatccg
       61 gcaccggcaa agacctcatc gcccgcgcca tccaccgcca cagcagccgc gccaagggac
      121 ccttcgtcac catcaactgc gccgcgctcc ccgaaagcct cctcgagtcg gagctgttcg
      181 gccacaagaa gggctccttc accggcgcgg tcaaggacaa ggacggtttg ttcaaggtgg
      241 ccgacggcgg cacgtttttc ctcgacgagg tcggcaacac ctccatggcc atccaggtca
      301 aactcctgcg cgtgctcgag gacaagaaga tcacgcccgt gggcgacacc cggccgctcg
      361 aggtcgatgt ccgtctcatc gccgccacca acgccgacct cgaggccgag gtcaaggccg
      421 cccgcttccg ggccgacctc ttctatcggc tcaacgtcat ccccatcgcc atccccgctc
      481 tgcgcgagcg ggccgacgac atcgacctgc tggcccagca cttcatcgac cgctgctgcg
      541 ccaagctcgg cgtgccggtc aagcgcctca ccgacgaagc ccgtctcctg ctccgcaatt
      601 atccctggcc cgggaacgtc cgcgaactcg aaaacacgat cgaacgggcc gctctcctca
      661 gccggtccga gacgcttgat gtcggcgatt tccccgacaa actccgcgag ccgtcggccg
      721 ccccggccgg tctcatccag cccgaccagc cggcgactcc gaccctcgag tcgatcgaga
      781 aggcctacat ccactacgtg atgagccaga ccgagggccg gaaagccgag gcggcgcgga
      841 tcctcgggat cgacgcctcc accctgtacc ggaagattca gcgctacaac ctgaccgact
      901 ttgtcacccg aaaggataaa ccgtccggca ctcctgacgg cgcctaggcg aagattgcac
      961 aggccgcggg aatgccgctt gcacatacca gggctaaagc gaaccgaaag ggaagaggat
     1021 ctggatatga atcggatacg gaacaatcgc ggtttcacct tgatcgagct gatgatcgtc
     1081 gtcgtcatca tcggcatcct cgccgccctc gccattccac gctacatgac cgccagcgcc
     1141 aagtccaagc agagcgaggc caaggccatc ctcaagcagg tctacgaggg cgagcgcgcc
     1201 tacttccacg cctccgagaa caacacctac tggacgccgg ccgcccccgc gagcgcgggg
     1261 aaccggaccg ccttcgccga attgtgcgtg gagatcggcg cctcggcccg ctacacctac
     1321 accatcacca cggccgccgg ctccttcacc gccaccgcca cggccaacat cgacgacgac
     1381 gcggtcctcg acacctggac aatcaacgag cagggcgtcc tcaccaacac cgtcaatgac
     1441 attctcgtct cctgatcccg tccgacgggc gtggggtcct ctgatggcca tcgatccgtg
     1501 cgacggcacg tctctccgga ggcgcgccgt cgcttcgttt tggcctcccc tcccgcccgc
     1561 cgccgctcct gcatcctgca aggactctcg caaattgccg agttccccgg ccgacaaaaa
     1621 ccgtcgaatc tgtcggcccc tcgccatagc ccgccggccc tcccgaaaac ctataccgcc
     1681 gcaaggcttt ccggcccccc aatctgttca attttccgac ggcaaaaggt atggactttg
     1741 cgataggatc agccgacaca gatgctgaag tgaaagctgg aacctgaatc accgtactat
     1801 aggaggtcag catgttctca accatccgca accgcaaagg tttcaccctg atcgagttga
     1861 tgatcgtcgt ggtcatcatc gggattctgg cggctttggc catcccgcgc ttcatgcagg
     1921 cgtcgatgaa gtccaagcag tcggaagcca agggtatcct gaagcagatc tacacgatgg
     1981 agcacgccta ccgccaggag catgacgtat acgtgaccga tctgaacacg atcggcgtcg
     2041 agctgcagcc gcagccgccg cgccgctacg gctacgtcat cgctctgaac gggaccgggt
     2101 tcacggccac ggcgaccgcg aacctggatg atgacgcgac catcgacacc tggacgattg
     2161 acgacggcgg cattctccag tgcaccgtca acgacgtcac gggttgatag gacgacttca
     2221 cacctcccta ccggaagggg cgcccgctcg cgggcgcccc ttctttttgc gcaactttcg
     2281 cccggccgcc gccgatacct ctattgtagt tgctttctcc cgccccacaa tgctaggata
     2341 ccgtatgacc aacctgcgct ttgtaccgct tgcgctggcg gtgctgtgcc tgccggccgc
     2401 cggcgccccg ggcgaaccgc ccccggagat ctggggcctg acccacctcg ccgactacct
     2461 ggccctcacc ccggccgaca tcagcttccg ccccgactac accgagcccg actccttccg
     2521 cctccaggtc ctctccggcc tcatggagaa ccccctcggc atgatcgact acgccgccgg
     2581 tctccgccgt gcctacacca aaggccagcc cgaggtgacg gccgcgctcc tgtcccgcga
     2641 tctcgcggtg gtcggccgcc ccgaccgcac cccgccgtac cgtccgaccg gaccggaaat
     2701 gcagcagggc tataacctgt tctacgccag ccccggcctg aaccagctca tggcccgggc
     2761 cggtctctac ctcgacgtca tcttcccccg gtccgccgag acggccctgg catcgctcag
     2821 ccccgcccag cggcggtttc tcaccgccga attcaaggaa ctgctggtca gtcacgcgga
     2881 ggaggagttc ttcacccccg agcagcttga ttcggtcgag aagatcgagg agaagtacac
     2941 ggaggagttc accgctttcg gctggcggat cgacaaggac ccgatcctca ccgccggttt
     3001 tgactgcctc cgcgacctga tgcccgaaat cgccgccctt cgccgcgacc tcgccgccgg
     3061 gaccctggac gccggcgccg tgatgaaagg tctcggccag atccccgaga aaggggtggg
     3121 cgaggaatcc tacctcggcc gccaacccgg ttggaaagtc ggcggccccg gccccgacta
     3181 ctacgagggg gactactggt tcatcttcga tttcggcggc gacgacgtct accgcctctc
     3241 ctacgacccg gcccgccccc acggcgtcat catcatcgat ctcgccggcg atgaccgcta
     3301 ccgcggcctc accgatttcg cgctcgcctc cggctgcctc tccgccggcc tgctcctcga
     3361 cttcggcggc aacgaccgct acgacgccca gtcgttcgcc ctcggctccg gctatttcgg
     3421 cttcggcctc ctctacgacg cggccggcga cgatcgttac gacggcgaca cgcacgtcat
     3481 ggccgccggc tcgttcggac tcggcctgct catcgacgag gggggccgcg actgctataa
     3541 cgccgctctc tacgcccagg gcttcggctt caccgagggc atgggcgtga tctacgacac
     3601 cgacggttcc gacagctact atgccggcgg caagtacaag gacatcctcc gctatgagga
     3661 ccactacctc tcgctctcgc agggcttcgg ctacggcctc cgcccgtgga cctcgggcgg
     3721 catcggcgcc atcatcgacg accgcggcag cgacgcctac tacaccgaca tcttcggcca
     3781 gggcacgagc tactggtggt cgctcggcgt catctacgat tcctccggca acgacaccta
     3841 caactcgttc cagtatgccc agggttgcgc tacccacatg accctcggcg tgctcatcga
     3901 cgacgccggg aacgacgcct acatcggcaa gggcctcatg cagggcgtcg gccacgacta
     3961 ctcctgcgga ctcctcctcg accgcgccgg cgacgatctc tacaccgcct gggacctctc
     4021 gcaggcggcc ggttccgcca acggcgtcgg catcctcatc gacaacgccg gcgccgaccg
     4081 ctacttcatc cgcaacgccg acaacacgca cggctacggc aacccccgtc gagatttcgg
     4141 ctcgatcggc ctgttcctcg acctcggcgg cgaagacctc tacaacggct acggcggcga
     4201 caacctctac tggcgcaccc cttcccaatg gggcggcggc atggacatcg aacgcgcccc
     4261 gcgcccggac agcgccgtca tcggggaggt gcagtgatgt tttcggattt caagaatcag
     4321 gacacccttc agaagcccag gaccgcctcg gacggccgtg cgtctcacgc tgacccggcg
     4381 gcccggcggc cgctgtggcg gcccggcggc cgggtgcgca ccctcatcgg cggcctcctg
     4441 ttggtgatcg tccttgcccc cgctctgagc gcgcagatcg acctccagca ccgcatcgac
     4501 tcgctgtttg tcatcgcttc ctccggcgag gtgcgctacc agaagttgac cggccctgcg
     4561 atggactcga tcgcggcttt tggccagccg gcggtcccat acctgatcga caagttcgac
     4621 acgaaatcgg cccgcgaccg ctggacgatc atctggattc tccagcgcat cggctctccc
     4681 gcggttcccg acctggtgcg ggctctcgat cgcgccgatc cgctcgtggt ctcgcgcgtc
     4741 gcctgggcgc tcggcgacat caaggacacc ggcgccgttc tcccgctcat cgccaagagc
     4801 acccacgccg cctggcaggt ccgcgaggag acgatcggct ctctcggcga catcggcgac
     4861 cggcgcggcg acaacgccat cctggccgcc ttgggcgact cgatcggcca ggtgcgcaag
     4921 gcggctgcgg tcgccgccgg caggctgaaa atcctcgagg ccgccgcgcc cctggtccac
     4981 cggttcggcg atcccttcta cggcgcccgc ctggccgcgg tcgacgccct cctgcagctg
     5041 gacaccgtgc tcagcctgcc cgtcctcatc gattctctgg cctcggccga cgccttcgtg
     5101 agcgccctct cggccgcggc cctcggccgc atcggcgccg accgcgcgct cgacgcgctt
     5161 ttccggcagg cgacggagga tcccgacccg gcccggcgcg tgccggccgc ctgcgccctc
     5221 atcgaggccg acccgctcga tcgctgccgc taccgcgaga aactgctcgc cgcgcagcct
     5281 gaccgcctcg ggcgcctgaa agtggagtcc gccctgaaaa cggcccgcca tgtcgaaaaa
     5341 ggaactgcac agtaagctcg accggctccg cggccacatc gtcgcaaaac ccgcggcccc
     5401 gccgcccgag gttctcccgg agcgctgccg ggtgctggcc gagcggctcg atggcgaggc
     5461 ggtctgcacc gcgcgcggcg gttttgtcct catccgcaaa cgctttccct ctgtctacac
     5521 ccacggcgag atcgcgctgg cggatttcgc cgcccgcacc gacttccccg cctccgcctt
     5581 caccgcctcc gacgacccgg cccgcctccc cgtcgccgac ctgcgcttct tcgacctcga
     5641 gaccatcggc ctcggcggcg ccggcacagc cgcttttctc gtcggcctcg gcgctctcgt
     5701 cgagggcggc ttcgaggtcc gccagtatct gctgcccgac tacgatgacg aggccgcgct
     5761 gctggccgcg gtcgccgagg aattctccga ccgcaccgtc ctggtctcct acaacggcgc
     5821 cgcgttcgac ctccccatcc tcctcgaccg cttcaccatc aaccgcgtcg gccgggagat
     5881 cccccgcgcc cgccacgtcg atttgctcca cgcggtccgg cgcctgttcc ggcggcgcct
     5941 gcgccagtgc gacctcggca atattgagcg cgatgtcttc gggttccgcc gtttcgacga
     6001 catccccggc tacctcgtcc cctccgtcta tttcgactgg ctgagcgatc acaaactcga
     6061 cgagatggag aaagtcctcc agcacaaccg ccacgacatc gtctcgctgt acttcctcgc
     6121 cgccgtcctc gccgaggtgt tcgagtcgcg aggggagagg ctctcggaga tcgacgatct
     6181 ccactcgctc gcccgcgtgt tcaaccgccg ccgcgatttc gatctggtcg tcgatctcaa
     6241 ccgccgggtg gagagtctcg ccggcactcc cgaccccctg gccgatcatc cggaccctcg
     6301 cgccgaccgc gcccaaccgg gcggtcaccc cggcgcccgt gcgctccccg ccgatgtcct
     6361 cttcttccac gcgcagactc ataagcgccg cggggagtat gacgaggctg tcgtgatctg
     6421 gaagcagctc gcgtcgtcaa tcccggcccc tcccgcaacc ggtcccgcta ccggtcccgc
     6481 gtctcctccc gccaccggtc ccgcgcctcc tcccgccacc ggtcccgcga ccggctcttc
     6541 tcatttgtca ttcccgcgaa ggcgggaacc caggcacacc ccgaccggag cttcggactt
     6601 gccgatcagg cacaacctcc ccgccgatca gaccccctcc ggtcccggcc tgacctcacc
     6661 cgccgatgcc ggaaccctcc tccgcgtcgc ctaccgcgcc tgcctcgaac tcgctatcca
     6721 ctatgagcac cgcgccaagg aattcgccct cgccctcgcc tatacccgcc gcgccgaatc
     6781 cctccccgac ctgaatccct cgcgtcggag gagtctgcag aagcgccggg atcgcctggt
     6841 gcgaaagcag gaggcgtgag ccggcggccc cgctgttctg attgcacttg acgcgcttgg
     6901 gcaaaagcga caaattgagc gctgttggaa tgcacgatcg ctaatcgcgg ggcgtcggct
     6961 ccattattgc gccaggagga tgtatgagcg acttcgccga ggatgatgtc accagaagca
     7021 tagcgccgct cgccgagggc cagattgtcg gccacttccg catcgagtcc cgcatcggcg
     7081 ccggcggcat gggaaccgtg ttccttgccc acgacatgga tctcgaccgc aaagtcgctc
     7141 tcaagcatct gtccccggag cttgcccagg acgagcagtt ccaggcgcgc ttccggcggg
     7201 aggccagaat cctcgccaac ctgaaccatc cgaacatcgt cggcatctac gaggtcttcc
     7261 aggcgggcaa ccgctcgttt atcgccatgg aatacgttga cggccgtccc ttgagcgccc
     7321 agcccgaccg gccgcttgct gagatttgcc agacggccgt tgagatcgcc tccgggctca
     7381 agaccgccca cgacgcgggt attgtgcacc gcgacatcaa accggccaac attctggtag
     7441 gccaggacgg ccgggtaaag atcctcgatt tcggtctggc caagcgtact gacgatcagg
     7501 acatcacccg caccgggggg accgcgggca ccttcgccta catgtctccc gaacaggtgc
     7561 ggggaacggc cgtcgacaga cgttcggaca tcttctcgtt cggagtcgtg ctgtacgaga
     7621 tggtcctcgg ccgccgcccg tttcgcggcg agtacgccgc ggaaatcgcg cacagaattg
     7681 tcgccgaacc gccgtcggcc ctcgatcccg ccgggaccgg catccctccg gaactgctcc
     7741 gcattatcgg ccggtgcctg gagaaggacc cggtggatcg gtatcaggaa accggccagg
     7801 tggtcgacga cctgaagcac ttccagcgcg tcctttccgg gtccggcgac ccctttgccg
     7861 tcatctcctc gccccggagc ggagtcggcg ccgcgccggt cgcggatgct ctcgacgcgt
     7921 cgcagattcc cgcgcgcccg cctcagccga aaccgctctt tctgcttttc ggcctgggcc
     7981 tcgccgcagt cggtgtggcg cttctgcttg ccttcacccc ccagccgcta tccgaactcg
     8041 agcgggctac gaccgcacct gcgcaggtgg aaaaggccgc ccttgacctg gcgcggcgtg
     8101 accacagcgg ccttttgggt ttccgatcct acaccgtcag cgatgccgac gccgagcttc
     8161 aggaaatcag ggagttcctt cgtccctccg accgcgagtg ggaggcgctc agccaatggc
     8221 ggcctgccca gttttaccgt accgctgccg tgagcgccga tcagcaggaa cggtacgacg
     8281 tgctcacgac cctcgatggc cggtgttttt ccttcagcca ttcgctgcgc cccgaccgga
     8341 ggtccgattc cgcgtccgtt gattcgctcc ggagcgcggc cgggcagtat gcgcaggcat
     8401 ttctcggccg ggacctcgat tccctgagag aaatgccggt ggccgtttcc ttctccgcag
     8461 gcgtcgccca aacgagcttt cagtgggtcg agcccgatac tctcctggcc ggggccgtgg
     8521 gagaagtccg tgcgacgttc tccggcgccg ccctcacgga cattcgctcg actgcggcgt
     8581 tccccgaggt tctgcgtcgc accatcgcca gccggggccc ggactggatc tctaccggca
     8641 ctttctgcgc ggtcacgctg gcctttttgg ctgcctgcat attcgtcacc aaaaagagga
     8701 tgtggcagtt cccgtccccg tcggtcctcg tcgccgctta cgccctcatg gtaatcggcc
     8761 tgctgggcgg caccggggcg tacgagaaat tcgctgatac cggcacggcc ggcgtgctga
     8821 cccatgtgct cctccttctc gtcctctttg ccttcttggc cgtcgccgtg tatctggtca
     8881 tcgccgccgt ttatggtgtg gtcgggcgtg tcaaaccggg cctcctggcc ggactcgcgc
     8941 ccctcctgaa ggggaggatg gagcgccctg ctcttgccgc cgccgcgagt accgggtttg
     9001 tctgcggcgc cgcgctgtat gtggtgtgca tgctctaccg ccattcggcc gcggtcctgt
     9061 tcgatacggc cggcagcctg tcgtcgtctt ccgagtatgt gaattccggc gtgcaggtgc
     9121 tgtctctgac catcctgggg ctgcctttca catgcgcggc ggtcggcctg ttcgttctcc
     9181 tggctgagat tctggaacac tatacgccgg tcggcaagag agcctggctg ctgttgaccc
     9241 tggtggttac ggtgctcgtg gctgccaacc agacgtccgc ggccggcgac agcgctgtgg
     9301 atttgccggc gctgtggctg gtgatcttca tctttgtctc ggtctggctg accaatcgct
     9361 acggcctgtt cgccggggct ctcgcggtcg tcgtttacaa cctggtggac agcgccgtca
     9421 tgttggccca ctatggccac ggagggttcc tcaaagactc cctgctgtgt ctccttctgt
     9481 ggggtgtgcc cgcggtctac gccacctact acgcggtgcg tcgcccggcc ccggccaagt
     9541 aggttcggag ccgcccgctc gcgaccgccg gcccggctgc gcggggccgg cgttttttcc
     9601 cgcaacacgc cgccgggacg gcactgcctg cgggcgcccg atttgccggc ctgattctcg
     9661 cccttcgtct caaccccttg ctcattccgc cgataatact catatgacag acgccaagac
     9721 gcccgccatg accgagcagg atctcttcca gatcatcctc cgggatcacg aggagttggc
     9781 ctcgctgccg cagtcgctgg ccgaggtact ccggctggcc cgcgatcctg attcttccgc
     9841 ccgcgacctg gccgccgtgc tgatgcgcga ccccggtctg acgaccaaac ttctccggct
     9901 ggccaactcc ccctatttcg gagccggccg ccggatcagt tcggtcaccc aggcggttat
     9961 gaccctcggg atgcgcgccg tgaccgcgct ggcgctctcg gcctcggtct acgacctcac
    10021 cggcaaatgg gccggggccg tcgaccgcct tcgcttctgg cgccactcgc tggcggttgc
    10081 gatcggcgcc cggagtatcg ccgaatcgat tcgctacgag tgttccgaag aggcgttcgt
    10141 cgccggcctc ctgcacgaca tcggtctgct cgtgctggaa aaatcgttcc ccgagcgcta
    10201 tgcccggctt tggcggcagg ccgaggcggg ggagagcctg cccgatctgg aggatcaggt
    10261 ctggggcacc aaccacgccc gtgtcggcca gttcctcctc gaacagtggc acatcccgga
    10321 ggcggtctgc tccgcggtcg gcaagcacca caccgagtac ccggccgatg accacgaccc
    10381 ggccttccgc cttccgcaga tcgtcgccct cgccaacgac atcgcccagt tcacgattgt
    10441 gaaggcgcgc ccgctccacg cccagcacat ggatcgccag gacatcctct gtggcaacct
    10501 caagatcgat gccgaccggc tgcagaagat ccgcgaggat ctgctctccc ggaccatgca
    10561 ggaggcggcc ttcctcgaaa tcgaaatcgg ctccaacgag gtgctcctca cggaggccaa
    10621 ccgcatcctc taccaccagt tcatcgcgct cgagcggctg ctgcgcgaga accgcgccat
    10681 gcagcgcgag atcgcccgcg gccagctcga aaaatccgcc ctcgagaccc tcaaaaccat
    10741 caccgccacc ttcaatcact acatcaacaa cgcggtcggc gctatcctcg gccgcgccca
    10801 gctcgtccag cacgatatcc gtcggggcac gttcgcccgg aatcccgaga gcgcgctcga
    10861 atcgatggag atcatcgtca acggcgtgaa gacgatccgc tcagtgatgg aggagctcac
    10921 caatctctcc gccttcaaga ccaccgttta ctacgacgac acgtacatca tcgacatcga
    10981 aaagaagatc cggaaaaaag tcgaagcgat cggcgacagc ccggccccgg ccgcgaagta
    11041 agcggttgga caccaatgga ttagatgatt tatcgaaagt cggagagaag ctctgaaggg
    11101 tcgccccgcg ggctcagtct tcccccacca cgatcctggt ccgcgttccc gtaatcgtca
    11161 gttccggccg cgctttcccc agcgccagct ccgccacagt cgtcgttccg gcctgcttca
    11221 gcgacagggg gtaggatgac tcgatcttgc cgtcgaccgc agtcgcccgc atgagcaggt
    11281 cgatgcccgg ttccagcgtc aggttgatca cgccgccggt tgagcggatc gccgatggtc
    11341 cctgcagcgg tcccgacacg ctcaccgtaa ttggtccgtt ggaattagtc aggctgatcg
    11401 gtcccgagaa gtcggtggcc tggatgggag cgaaggagtt gccggcctcg atccgcccgg
    11461 tgatgtcgga gatcacaatc ggggcgtggc tgttctcggc cgtcaccgac cccttcacgt
    11521 acgcgatctg catcggctgg taccggctgg tcagccggca gtttccctcg gagtgctgga
    11581 cctccacctg gccgttggag ttgtcgacgc tgatatcccc ttggtggtag ctgatgcgca
    11641 cctggccgct gttgcggatg tcgacgggcc cggcgcactc ggccagctgc accgatgcgt
    11701 actcgttgtc aatcgtcacc tccccatcgc agccctgcag ctccaccggc gtacggcgcg
    11761 cggtgatcgc aatatgcccc tcgactccgg tcagacgcac cgatcccatc tggcacgtca
    11821 gccctacccg ccccacgatg tcgctgccgg tcacgtcggc gtgttcggtg gtgagcatca
    11881 catcgttttc caggctttcg agcacaacgc tgccgaacgg acttgtcacg atgagcgggt
    11941 tgtcctgcgg caccatgacg gtgagagtcc ggttcgcggc cttctggcgg gtatcgcgca
    12001 actcgggcat caggtagccg acctcgtagc cgctgccgga ccgttcgagc cgaaggcgga
    12061 cttcttccgc gagcgcctcg gccgctttgc gcgaatcggc cccgagcgtg atctctcccc
    12121 gcaccaggag cagctcccgg tcccacccga tcaagcgcag gtcgccgttc gggttggtga
    12181 tcgtgatcgg cgcgcgcgag gcgacccgca ggctgtcgcc gtactcccgg accacctcgg
    12241 tctttccccc ggcgcccgtg aacacgatgt tgcggtcgcc caccaccacc ggcgttcgag
    12301 gcggggccgg tggtttggcc gacgcgacat agacctgcgg gatcaccgca cacatcgaat
    12361 ccccgaacag cgaatcaagg tactccagct tggccagctg ggcggggtcg aaatcaaacg
    12421 tgaaatcgaa atccggcacc gccacctgga cgttcccgtc gcgccccagc agtctgacat
    12481 agacctgccg gagcttctcg gcgtcgacct gcccggagtc gctggcttgc tggaaacaga
    12541 aatagagctc cggcggcagc ccgtcatttt ccgaggggag gtcggcgagc tccgattcga
    12601 gctggatggt gatggcctcg aggctgcgcc ccagattctc gctcagggca taggcgcgcc
    12661 ggtccgcccg caccgcctcc cgctgggagt cgagggccgc gcgcacgcgg ctcaggtccc
    12721 cctccagttg cagggcgttt ttcaggtacg cgcccgcggc gagccgctcc atcaacacct
    12781 gcagctcttt ctgctccgcg gtcaccgtgc tcgccgccgt ttgcccgctc cgaccggcgg
    12841 agcgcgactt gacggccgcc tccgcgaggt accggttgta gtccttcatc acctctttga
    12901 gctcgttgag cagctcgagg tactcgcggc cgagatctcg gacctcgcgg ctgaggcgct
    12961 gcagctcctc gcgccgggcg tcttccgcac gatcctgatg ccggtcgccc gctggcggcg
    13021 gcggaggaag cggggcctga gcccacaccg ggccggccgg ggagaaacct cccataagaa
    13081 cacacatgac cgcgaccgcc gtcgccgctc ccgcccttcg gcctcggcgc gcgccgc
//
LOCUS       DAOPOE010000087        12693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5751_length_12693_cov_5.150498, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000087 DAOPOE010000000
VERSION     DAOPOE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5751_length_12693_cov_5.150498"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..4277
                     /gene="porU"
                     /locus_tag="PKM94_10830"
     CDS             393..4277
                     /gene="porU"
                     /locus_tag="PKM94_10830"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOD67167.1"
                     /translation="MLRFQGRVYARLAMDIRLYRLLLLSTFALILSLPVAASADGVNL
                     VYSDRTGLHAVVTVDPERLESAVEADSLPGYYRLLMVGVPFGAEVELASADAHSRSVR
                     PVRLTDGRKMSRQARPLVEVSEPFTVRGRQMVTVRVNPAGPDGGCADSIDVRLTFRGG
                     LMEGAAPRDPWFDRVFGERVVNYREFRTWPAPEALKTKAAAPGPFAAGETWYKITVNR
                     TGLCRVTGSQLQQAGLELSGLASDALCLFNAGGRRLPLNNATPRPEFRQAAITVDDGG
                     DGRFDAADAILFFADAGDRWVYAPATAPFWENNPYETKNVYWLTVREGQGVRMATETV
                     DPSAPADTTITTFTRVMHLEQDNLLKEDAGGAIDDYYIWYWTTGPTVQLSAPSVGPAP
                     GTSAQLYCRASTRDPDVSPEIGYVTARVNGAAAAPNYQNQFHCSFRVDNLTDGLNTVN
                     LTMWGADGVLPYLDYVELTYQSFLAPLGGVLDVPLGGFEGTARLEAVDNFSGDLAVLR
                     LDDPGRPVRLAGYTRAAGTIAFTAALAGEGPNRFYLAETAGAFGPLGIERSTPADLYA
                     IGEQTDMIIVTSRQLVPALAEYVERAAGRGISSRVVAVEDIMENFSFGMYDPTAIRDY
                     LKYAYENYPSPPPHTVLLAGDGSFDYTNALGTGVPNVVPPYIRPDDRSAADDNYVYFG
                     RYGILDSDTSYILVGDRGLDMMVARWPVQTAGEIATIVEKSRRYETSAALGLWRSVIT
                     VVADDEVADNQTSETFHVEQAEELLAGHVPRTLHQKKIYLYDFPRVGREKPGANDAIV
                     RQINEGAVVVDYIGHGNPDVWAHEHVFWRDRDIPRLSNSDRLPLFFAASCAIGAFDDP
                     AREAMAEELVVYPNGGGIAVISAMREVWSTQNADLNQKVFDLLLTTDSLTIAEALYMG
                     KLERQYNGGSVPYEILNDRCYEYFGDPLVRLGLPRLRIAFDNPPDSLVALGRSTIAGR
                     VLDREGRPVAADGRLVIEAYDSERERQYRLASGNVVSYRLTGPAIYRGTATIRSGEFE
                     FAFVTPLDVGYGGTGAQIMTYAELAGTDGVGVIDSLSIAAAVDTTHRDETGPEIAYGF
                     VHSVGFSDGGTVATDDVLRVTLADSSGINLTGGLGHGITLVLDDRSETMADLTDRFAC
                     DQDDYTRGQLEYPLAGLSPGRHTFKIKAWDNANNSATAAFTATVAAAGGPALVDLLNY
                     PNPMRERTRFYYRLTTAVERVCLEIFTLAGRRIWVHEEFPTDPGYYDDIEWYGTDFAG
                     DRVATGVYVYKATAYPRGGAAVETFGKVVVVN"
     gene            4299..7061
                     /locus_tag="PKM94_10835"
     CDS             4299..7061
                     /locus_tag="PKM94_10835"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD67168.1"
                     /translation="MVRKLGVLFGLVAAAVLGASDSRAEISNAAVLFLRIAPGARAAG
                     MGEAFVAVADDATTTHWNPAGLGAYPLADGWTVARVPADLLPLKAIAAVKQGGGSDYT
                     AYEIWAISGPKGLVRFDNRAWHTGEEFSTRTDQTVRGIVASYFKVQDEDLLNQMVARV
                     ARANAEMSREELALLRDSILAALPEGYRDTEFLTQRLDSVMTAYDEVRLRWPNVQEAR
                     KALRDGLDDGALSSGEADKLSVAIERARMRFIPEEVTIPYTVVFDGEPTAIAAKEKLL
                     VVGTSEGLFTYDGKSWRTFTEKDGLPSDEIRSLHASGAQFFVGTAEGLVMFTGRDIAA
                     VDTTGTLPAGYVSAIGADGTSRVWAVIDGKLYRYDGASWSRGRAYTIAVGDTLEKIAE
                     RFALYGTASERQSLVDLIKELNPRGPQAPAAAAAAAPAQGEPLDITKLAEMAADTAAA
                     DSAAPAAETAAAGEAATAEEAPAAESGGEPDWANLPPGDVLILPFAVEIKGNVNSIAA
                     RQDELFLGTDFGLLYFDGEKWRMPGWSEYTAPQNQTVEDVLGQRAATNGEARERYLLA
                     IREVNGIESTVDSGAAIRIPQNALAAKVNLVRQRGASVYFATAEGLRVLEDGRVQRID
                     EKGMGEANTIYMDAVEDELWLASDREIVIKANGRKEFSLMHVNWLPELASDMYYDFIA
                     GVFQVGDVGTAGFNVTYLTYGEITNTNSLGQVIGSFRSFDVAVTGSFGFPVSQKLKVG
                     VSAKLIHSHLSDVGTEKEKGSGTSTGVAADLGLLYQWTPRLNIGLAITNLGPDVIYID
                     AAQADPLPRNLAVGLAYKVLHTDYYTFLVTAEMNKMLVGIDLSSVKDEISSAIFNGGV
                     EFSYANLIAGRAGYIYDQEGQVKAVTLGAGLKPLDWLRADFSYIPSSKESVLDNTLRF
                     SLSIVP"
     gene            7087..10413
                     /locus_tag="PKM94_10840"
     CDS             7087..10413
                     /locus_tag="PKM94_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67169.1"
                     /translation="MREIGRLGRLALAAVLVPAAVAAGDGLLGIRNVQHPGETAAGEN
                     RVHHRCLTPPRNRAADRGLEREHPWSLPKQARAADFADTVRCLVLRFNFQYEATDDPH
                     TSGRGRMLIRGPEADSAANPTYFGDAGNLVDPPPHDSAYFDAHLRALARYWEVVSDSA
                     ITLAWDIYPPARDSVYELPHPMSYYGQCDFDSVIAGLERYFIDGIRLADTVSPEIDFG
                     RYDAIFMFHAGSDRQNDFGPNSVGVPYTCSDLFTGFILFGDSLAVDEGANWVKTALLM
                     PETVNQDNRATTMNAVLAHEFGHQLGLVDLYSTATFMSQLGDFALMDNNGFGTTVDFG
                     LPVGGVYGTMPLYPCAWSRAYLGYVQVYDFREGTRIPLAAAQMVQEGIKVARIPITEK
                     EYYLLENRIIEYDGLPTGQKQDTATGVLQGPVQVIITPQGDTVAGEFTREYDFLLPGS
                     GMLIYHVDESVAGLDYDGDGVNNFDDNQLQWDPARRFISLVEGDGIVNFGGYYRAGWG
                     AAADMYRDDRNRSFTPNTNPPTIDNTGNNTHIRITGITRKIDTLGASIVVRDSLMLFD
                     VETENLVAGFPVRVGESTLPLSPIAADLNGDGTDEIIAAAGKRVVAFTTDGEGYFHKF
                     FGTSDELFFDSAKARVHPGRPYPVPVFATAQSGQVLSAGPVAGAFGNLNDTLHIAVGY
                     RRESDSLGRNGNVIIYRATDFDQNGLAVSAAGFATNGRGFPAALAFGDVLWVLTSEGH
                     VYRRSSLMDSAIELGSFPMDEYHGICRAGSGLVLVGGDETHTVWHAIDSTGASNALTV
                     DGHYRFGPVLADVDRDGVPEAAGFSAEGDAILIAVDTTVSPAALEVKAQKRTGLSVRA
                     NPAAADMDDDGYPDLVVGGVNQVAAFDRQLTLLADFPKELDDRFPEAEVIAAPVVGDI
                     QRGGRPEVIAPTEPGNIYAYGDRAATGFPLSAGEFCIGSPLVVHDSLGGKLGYLGLDG
                     WFYLWHTNLDSVRNYWPMAGADPAGSFALDGSKLGEPRQYAAGFDGKRFYNYPNPVTE
                     GRTTIRYFLGRAARSVGLTIYDLSGTEVAALQGPTAGGVDNELEWVCGKVTPGVYRCR
                     IEVDFGGETETAFTDIAVLK"
     gene            10426..11343
                     /locus_tag="PKM94_10845"
     CDS             10426..11343
                     /locus_tag="PKM94_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67170.1"
                     /translation="MRRGIAGWMMCLGAAGLAAGGAAAVELTPSPLEQAQVEVRYGLA
                     AAGNLDRWAAAELAPGDTGQAGTLPGEKSPVKAMLLSLAVPGAGQWYYGSRIKPFVFL
                     GLEALAWGLHAHYHYNGEDMTDEFEAFNREHWSQQAYADYLNWAYDGLTIKDLEDTVI
                     PGLTHHLPDTRTQQYYEMTGKYNQFAWGWDDANLQGLMLYADYSQSNPPPKIDQGVPA
                     SPRRDKYEDMRGDANHEFKKAGRMLVGVVVNHVVSGLEAFFMTRHRNRQLRGDDEAEE
                     FLSNLSIEPSLRSVYRFQDTPYVQVNYSF"
     gene            11357..12178
                     /locus_tag="PKM94_10850"
     CDS             11357..12178
                     /locus_tag="PKM94_10850"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOD67171.1"
                     /translation="MRIRVAIALGIGLVAGGAVCAAAVGAAGVRPAPVQTSLATQIRA
                     DGLAWRPTAAVTEWEDDGTLTPAPKENRPQEGKKKSPVKAAALSALVPGLGQLYNDRD
                     IKARCFFAAEGLTWVAFGAFRLYGDWKKDDLIAYAAVHANAQLEGKSDQYLDWVGFYD
                     SIDDFNRAGRVGDPDRRYLPDVPEYHWVWPSDAERETFRSLKNSSREAYRRGDWMLWA
                     AVINRVVAVIDAVLDARRANRVVDEGWTIGGVRYDLEFHPTSPTRQVALTVYPGF"
     gene            12186..>12693
                     /locus_tag="PKM94_10855"
     CDS             12186..>12693
                     /locus_tag="PKM94_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67172.1"
                     /translation="MRAGAVSVGAAAAAIALSCGGPGGAGDETAAPPAPAGVIVEQVI
                     AGEVLGLPLQSPWGLAAGTDDNVYCVDRGNNRIIKFSRALQPIRDAGGAGSEAGLLSR
                     PGYAAVDNQLNVWVADEGNQRISRFNSDLLFVDATEFYNADDPLQFGEPGGVAVSAHG
                     ELWAGDRQR"
BASE COUNT         2183 a         3696 c         4769 g         2045 t
ORIGIN      
        1 ggcatgtttg tggaaatgag cgaggaactg gcgcgcgaac tgagccggca gccgtgagag
       61 agtggatgcc gggagcagca aatgtgcagg agatttcacg gtttccggca cgccgggcgc
      121 gacggcggcc gggaccaggg gcgggttatc gtgtttggcc ttaagcactt agtgcaggat
      181 atggtgatga ggatctttat tggccgaggt cttgcgtaag caaagcgcag aaagagcgcc
      241 gatagcgctt gacatgtatt agggaacttt ctatccttaa acgatttata cagctgtgga
      301 ggagcgacta tcgacctacg gttactgaag gtttaagtta actcaatttg gatcggtgca
      361 attgcgttaa ggcccgcaga ccgtagttcg aagtgcttcg gttccagggc cgggtgtacg
      421 cccggttggc tatggacatt cggctgtata gattgctcct cctgagcacg tttgccctca
      481 ttttgagtct gcccgtcgcg gcctcggccg acggcgtcaa tctggtctat tcggaccgga
      541 ccgggctcca tgcggtggtg acggtcgatc cggaacggct ggagtcggcc gtggaggcgg
      601 attccctgcc cgggtactac cggctgttga tggttggggt accgttcggg gcggaggtgg
      661 agttggcatc cgcggacgcg cacagccggt cagttcgccc ggtgcgcctt acggacgggc
      721 ggaagatgtc gaggcaggcg cggccgctgg ttgaggtgtc ggaaccgttc acggtacgcg
      781 ggcggcagat ggtgacggtg cgggtgaacc cggccgggcc cgacggggga tgtgccgatt
      841 cgatcgatgt gcgcctcact ttccggggcg ggctgatgga gggggcggcg ccccgggatc
      901 cgtggttcga ccgggtgttc ggggagcggg tggtcaatta ccgggagttc cgcacctggc
      961 cggcgcccga ggcgctgaag acgaaggcgg ccgctccggg accgtttgcc gcgggggaga
     1021 cgtggtacaa aatcacggtg aaccgcaccg ggctgtgccg ggtgacgggg agccagttgc
     1081 agcaggcggg gctggaactg agcgggctgg cctccgacgc gctctgtttg ttcaacgccg
     1141 ggggacggcg gctgccgctg aacaacgcga cgccccggcc ggagttccgc caggcggcga
     1201 tcacggtcga cgacggcggg gacgggcggt tcgatgcggc cgacgcgatc ctctttttcg
     1261 ccgacgccgg ggaccgctgg gtctacgcgc cggccaccgc gccgttctgg gaaaacaatc
     1321 cctacgagac gaagaatgtg tactggctga cggtccggga ggggcagggg gtgcggatgg
     1381 ccacggagac ggtcgatccc tcggcgcccg cggacaccac gatcacgact ttcacccggg
     1441 taatgcatct ggagcaggac aacctgctga aggaggacgc cggcggggcg atcgacgatt
     1501 actacatctg gtactggacg accgggccga cggtgcaact gtcggcgccg agcgtcgggc
     1561 cggcgcccgg aacctcggcg caactgtact gccgggcctc gacgcgcgat cccgacgtct
     1621 ccccggagat cggctacgtg acggcgcggg tcaacggcgc ggccgcggcc ccgaattacc
     1681 agaaccagtt ccactgcagt ttccgggtgg acaatctcac cgatgggctc aacacggtga
     1741 atctcaccat gtggggggcg gacggcgtgc tcccctacct ggactacgtg gagctgacct
     1801 accagagttt cctcgcgccg ctgggcgggg tgctcgacgt gccgctcggc gggttcgaag
     1861 ggacggcgcg gctggaggcg gtcgataatt tcagcgggga cctggcggtt ctccggctgg
     1921 acgacccggg ccggccggtg cggctggccg ggtacacgcg cgcggcgggc acgattgcct
     1981 tcaccgcggc gctggccggc gagggaccga accgattcta cctggccgaa acggccggcg
     2041 ccttcggccc cctgggaatc gagcggagca ccccggccga cctgtacgcg atcggcgagc
     2101 agaccgacat gatcatcgtg acctcgagac aactggtccc ggccctggcc gagtacgtgg
     2161 agcgggcggc cggccggggg atctcaagcc gggtggtcgc ggtcgaggac atcatggaga
     2221 atttcagctt cgggatgtac gatccgaccg cgatccggga ctacctgaag tacgcctacg
     2281 agaactatcc gtcgccgccg ccgcacacgg tgctgctggc gggggacggc agtttcgact
     2341 acaccaatgc cctgggaacg ggagtgccga acgtggtgcc gccgtatatc cggccggacg
     2401 accggtcggc ggcggacgac aactacgtct atttcggacg gtacggcata ctcgattcgg
     2461 atacgagcta cattttggtc ggggaccgcg ggctggacat gatggtggcg cgctggccgg
     2521 tgcagacggc gggggagatc gcgacgatcg tggagaagag ccgccgctac gagaccagcg
     2581 cggcgctcgg cctgtggcgg tcggtgatta cggtggtggc cgacgatgag gtcgccgaca
     2641 atcagacgag cgagacgttc cacgtggagc aggcggagga actgctcgcg gggcacgtgc
     2701 cccggacgct ccaccagaag aagatctatc tgtacgattt tccgcgggtc gggcgggaga
     2761 agccgggggc caacgacgcg attgtgcgcc agatcaacga gggggcggtg gtcgtggatt
     2821 acatcggcca cgggaacccg gacgtgtggg cgcacgaaca cgtgttctgg cgggaccgcg
     2881 acatcccccg gctgagcaac tccgaccggc tgccgctgtt tttcgcggcc tcgtgcgcga
     2941 tcggggcatt cgatgatccg gcgcgcgagg cgatggcgga ggaactggtc gtctacccga
     3001 acggcggggg catcgcggtg atttcggcga tgcgcgaagt atggtcgacg cagaacgccg
     3061 acctgaatca gaaagtgttc gaccttctcc tgactacgga cagtctcacg atcgccgagg
     3121 cgctgtacat gggaaaactc gagcggcagt acaacggggg gagcgtaccc tacgagattc
     3181 tcaacgaccg gtgttacgag tatttcggcg atcccctcgt gcggctcggt ctgccgaggc
     3241 tgcggatcgc gtttgacaat ccgcccgaca gtctcgtggc cctggggcgg tcgacgatcg
     3301 ccgggcgggt gctggatcgg gagggacggc cggtggcggc cgacggccgg ctcgtcatcg
     3361 aagcctacga ttcggagcgc gagcggcagt accggctggc gagcgggaac gtggtgtcct
     3421 accgcctgac cggccccgcg atctaccggg gcacggcgac gatccgcagc ggcgaattcg
     3481 aattcgcctt cgtcacgccg ctcgatgtcg gctacggcgg aacgggggcg cagatcatga
     3541 cctacgccga gctggcgggg acggacgggg tcggggtgat cgactcgctc tcgattgcgg
     3601 cggcggtgga cacgacgcac cgcgacgaga ccgggccgga gatcgcctac gggtttgtcc
     3661 actcggttgg tttcagcgac ggcgggacgg tggccacgga cgacgttctc cgggtgacgc
     3721 tggcggattc ctcggggatc aacctcaccg gcggtttggg gcacggcatt acgctggtgc
     3781 tggacgaccg gtcggagacg atggcggacc tgacggaccg gtttgcctgc gaccaggacg
     3841 attatacgcg ggggcagttg gagtacccgc tggcggggct gtcgccgggg cggcacacgt
     3901 tcaagatcaa ggcgtgggac aacgccaaca attcggcgac ggcggcgttc acggcgacgg
     3961 tggcggcggc cggggggccg gcgctggtcg acctgctcaa ctaccccaac ccgatgcggg
     4021 agcggacccg gttttactac cggttgacga cggccgtgga gcgggtctgc ctggagattt
     4081 tcaccctggc ggggcggcgg atttgggtgc atgaggagtt cccgacagat ccgggctact
     4141 atgacgatat agaatggtat gggaccgatt ttgccgggga ccgggtggcc accggcgtgt
     4201 acgtatacaa ggccacggcc tacccgcggg gcggggcggc ggtggagacg ttcggcaaag
     4261 tagtagtggt gaattaatag agacaggagc aaagagcgat ggtcaggaaa cttggagtcc
     4321 ttttcggtct ggtggcggcg gcggtgctcg gtgcatccga cagcagggcg gagatttcga
     4381 acgccgccgt gctgtttctg cgcatcgcgc ccggagcgcg ggcggcgggc atgggcgagg
     4441 cgttcgtggc ggttgcagac gacgcgacga cgacgcactg gaacccggcc ggcctcgggg
     4501 cgtatccgct ggcggacggc tggacggtgg cccgcgtgcc cgcggacctg ctgccgctga
     4561 aggcgatcgc ggcggtcaag cagggcggcg gctcggacta tacggcctac gagatctggg
     4621 cgatttcggg cccgaaaggg ctggtgcggt tcgacaaccg ggcctggcac acgggcgaag
     4681 agttcagcac ccggaccgac cagaccgtgc gcggcatcgt ggccagctac ttcaaggtgc
     4741 aggacgagga cctgctcaac cagatggtgg cgcgggtcgc gcgggccaac gccgagatgt
     4801 cgcgcgagga actggccctg ctgcgggaca gcatcctggc cgcgctcccc gaagggtacc
     4861 gcgacaccga gttcctgacc cagcggctcg actcggtgat gacggcctac gacgaggtac
     4921 ggctgcgctg gccgaacgtc caggaggcgc gcaaggcgct ccgcgacggg ctggatgacg
     4981 gcgcgctctc gagcggcgag gcggacaagc tgagcgtcgc catcgaacgc gcccgcatgc
     5041 gctttatccc ggaagaggtc acgattccct acacggtggt gttcgacggc gagccgaccg
     5101 cgattgcggc caaggagaag ctcctcgtcg tgggaacctc ggaaggactg ttcacctacg
     5161 acggaaagag ctggcggacg ttcacggaaa aggacgggct gccctcagat gaaatccggt
     5221 cgctgcacgc gagcggcgcg cagttcttcg tggggacggc ggaggggctg gtgatgttca
     5281 cggggcgcga tatcgcggcc gtcgacacga ccggcactct cccggcgggc tacgtctcgg
     5341 cgatcggggc cgacggcacg tcgcgcgtgt gggcggtgat cgacggcaag ctgtaccggt
     5401 atgacggcgc ctcgtggagc cggggccggg cctacacgat tgcggttggc gacacgctgg
     5461 agaagattgc ggagcgcttt gcgctctacg ggacggcctc ggagcgccag tcgctcgtcg
     5521 atctgatcaa ggagctgaac ccgcggggcc cgcaggcgcc ggccgcggcg gcggccgcgg
     5581 cgccggccca gggggagccg ctcgatatca ctaaactggc cgagatggcg gcagacacgg
     5641 cagcggccga cagcgcggcg ccggcggcgg agacggccgc ggcgggcgag gccgcgacgg
     5701 cagaggaggc tccggcggcc gagagcggcg gcgagccgga ctgggcgaat ctgccccccg
     5761 gggacgtgct cattctcccc ttcgcggtgg agatcaaagg aaacgtgaac agcatcgcgg
     5821 cgcggcagga cgagctgttc ctcgggaccg acttcgggtt gctgtatttc gacggcgaga
     5881 agtggcggat gccggggtgg agcgagtaca cggcgccgca gaaccagacg gtcgaggatg
     5941 tcctgggcca gcgggcggcg accaacggcg aggcccggga gcggtatctc ttggcgatcc
     6001 gggaggtcaa cgggatcgaa tcgacggtgg actccggcgc ggcgatccgg attccgcaga
     6061 acgccctggc ggcgaaggtg aacctggtgc ggcagcgcgg cgcgagcgtc tatttcgcga
     6121 ccgccgaggg gctgcgcgta ctggaggacg ggcgggtcca gcggatcgac gagaaaggga
     6181 tgggggaagc gaacacgatc tacatggacg cggtggagga tgagctgtgg ctggccagcg
     6241 accgggagat cgtgatcaag gccaacggcc ggaaagagtt ctcgctcatg cacgtgaact
     6301 ggctgccgga actggccagc gacatgtact acgatttcat cgccggcgtc ttccaggtgg
     6361 gggatgtcgg cacggccggg ttcaacgtga cctacctgac ctacggggag atcaccaaca
     6421 cgaactcgct cggccaggtg atcgggtcgt tccgctcgtt cgacgtggcg gtgaccgggt
     6481 cgtttgggtt cccggtgtcg cagaagctga aagtgggggt gagtgcgaag ctgatccact
     6541 cgcacctgtc ggatgtcggg accgagaagg agaagggttc ggggacctcg acgggggtgg
     6601 cggcggatct cggtcttctg taccagtgga cgccgcggct gaatatcggg ttggcgatca
     6661 ccaacctcgg tccggacgtg atctatatcg acgcggccca ggccgacccg ctgccgcgca
     6721 acctcgcggt cgggctcgcc tacaaggtcc tccacaccga ctactacacg ttcctggtga
     6781 cggcggagat gaacaagatg ctggtgggga tcgacctgtc gtcggtcaag gacgagattt
     6841 ccagcgcgat attcaacggc ggcgtggagt tctcctacgc caacctcatc gccggccggg
     6901 ccgggtacat ctacgaccag gaagggcagg tgaaggcggt gacgctgggg gcggggctga
     6961 aaccgctgga ttggctgcgg gcggattttt cgtatattcc gagcagcaag gaatcggttc
     7021 tggataacac gctgcggttc tcgctgtcga ttgtgccgta agcgggccag ggaaagtcag
     7081 gcggttgtga gagagatagg acgtctcggt cgattggcgc tagcggcggt gctcgtgccg
     7141 gcggcggtgg ccgccggcga cggcctgctg ggaatccgca acgtgcagca cccgggggag
     7201 acggcggcgg gcgagaaccg ggtgcaccat cgctgcctca cgccgccgcg gaaccgggcg
     7261 gccgaccggg ggctggagcg cgaacacccg tggtcgctgc cgaagcaggc gcgggcggcc
     7321 gatttcgccg acaccgtgcg ctgcctcgtg ctccgattca attttcagta tgaagcgacg
     7381 gacgatccgc acacgagcgg gcgcggccgc atgctgatcc gcggaccgga ggcggactcg
     7441 gccgccaacc cgacctattt cggcgacgcc ggcaacctgg tcgatccgcc gccgcacgat
     7501 tcggcgtatt tcgacgccca cctgcgggcc ctggcgcgct actgggaggt cgtgtccgac
     7561 agcgcgatca cgctggcctg ggacatctac ccgccggcgc gggactcggt ctacgagctg
     7621 ccgcacccga tgagctacta cgggcagtgc gatttcgaca gcgtgatcgc cggcctcgag
     7681 cggtatttca tcgacgggat ccggctggcc gacacggtgt cgccggagat cgacttcggc
     7741 cgctacgacg cgatcttcat gttccacgcc gggagcgacc ggcagaatga tttcgggccg
     7801 aactcggtcg gcgtgcccta cacgtgcagc gacctgttca cggggttcat cctgttcggg
     7861 gattcgctgg cggtggacga gggggcgaac tgggtgaaga cggcgctgct gatgccggag
     7921 acggtcaacc aggacaaccg ggcgacgacg atgaacgcgg tcctggcgca cgagttcggg
     7981 caccagctcg ggctggtcga cctgtacagc acggcgacct tcatgtcgca gctgggggat
     8041 ttcgcgctca tggacaacaa cgggttcggg acgacggtcg atttcgggct gccggtcggc
     8101 ggcgtctacg ggacgatgcc gctctacccc tgcgcctggt cgcgggccta cctcgggtat
     8161 gtccaggtct acgacttccg cgagggcacg aggatcccgc tggcggcggc gcagatggtg
     8221 caggagggga tcaaggtggc gcgcatcccc atcaccgaaa aggaatacta cctgctggag
     8281 aaccggatca tcgagtacga cgggctgccg acggggcaga agcaggacac ggcgaccggc
     8341 gtgctccagg ggccggtcca ggtgatcatt acgccccagg gggacacggt ggcgggggag
     8401 ttcacccgcg agtacgactt cctcctgcca ggctcgggca tgctcatcta ccacgtcgac
     8461 gagagcgtcg ccggactcga ctacgacggg gacggggtga acaatttcga cgacaaccag
     8521 ctccagtggg atccggcgcg gcggttcatt tcgctggtgg agggggacgg gatcgtcaat
     8581 ttcggcggct actaccgggc ggggtggggc gcggcggccg acatgtaccg ggacgaccgc
     8641 aaccggtcgt tcacgccgaa cacgaacccg ccgacgatcg acaacacggg caacaacacc
     8701 cacatccgga tcacggggat cacgcgcaag atcgacaccc tcggggcatc gatcgtcgtg
     8761 cgtgattcgc tcatgctgtt cgacgtggag acggagaatc tcgtggccgg gttcccggtg
     8821 cgggtcgggg agtcgacgct gccgttgtcg ccgatcgcgg cggatctcaa cggggacggg
     8881 accgacgaga tcatcgcggc ggcgggcaag cgggtggtgg cgttcacgac ggacggggag
     8941 ggatatttcc acaagttttt cggcacctcg gacgagctgt tttttgactc ggccaaggcg
     9001 agagtgcatc cggggcggcc ctatccggtg ccggtgttcg cgacggccca gtcagggcaa
     9061 gtcctttcgg ccgggccggt tgccggcgct ttcgggaatc tgaatgacac gctccatata
     9121 gcagtagggt accgaagaga gtcggattca ttgggcagga acggcaatgt aatcatctat
     9181 cgtgctacgg acttcgatca gaatggccta gctgtatctg ctgccggttt cgcgacgaat
     9241 ggtcgaggtt tcccggcggc gttagctttt ggcgacgtct tgtgggtgct gaccagcgag
     9301 ggacacgtct accgcaggtc ttctttgatg gattcggcaa tcgagctggg gtcgtttccg
     9361 atggacgagt accacggtat ctgccgggcg gggagcgggc tcgtgctggt cggcggcgat
     9421 gagacgcaca cggtctggca cgccatcgac tcgaccgggg ccagcaatgc gctcacggtc
     9481 gacggccact accggttcgg gccggtgctg gcggatgtcg accgggacgg cgtgccggag
     9541 gcggcgggct tttccgcgga gggggatgcg attctgatcg cggtcgacac caccgtgtcg
     9601 ccggcggcgc tggaggtgaa agcgcagaag cgcaccgggc tgagcgtgag ggccaacccg
     9661 gcggcggcgg acatggatga cgacggctat cccgacctgg tggtcggcgg cgtgaaccag
     9721 gtggccgcgt tcgaccggca gctcacgctg ctggcggatt tcccgaaaga acttgacgac
     9781 cggttcccgg aggcggaggt gatcgcggcc ccggtggtcg gcgacattca gcgggggggg
     9841 cggccggagg tgatcgcgcc gacggagccg gggaatattt atgcctatgg cgaccgcgcg
     9901 gcgaccgggt tcccgctttc ggccggcgaa ttctgtatcg ggtcgccgct ggtggtgcac
     9961 gactcgctcg gcgggaagct cgggtacctc ggcctcgacg gctggttcta cctgtggcac
    10021 accaatctcg acagcgtgcg gaattactgg ccgatggcgg gagcggatcc ggccggatca
    10081 ttcgcgctcg acggttccaa actcggcgag ccgaggcagt acgcggcggg attcgacggg
    10141 aagcggttct acaactaccc caacccggtg acggaagggc ggacgacgat ccggtatttt
    10201 ctcggacgcg cggcgcgctc ggtggggctg acgatctatg atctgtccgg caccgaggtg
    10261 gcggcgctgc aggggccgac ggcgggcgga gtcgacaacg aactggagtg ggtctgcggg
    10321 aaggtgacgc cgggcgtcta ccggtgccgg atcgaagttg atttcggcgg ggagacggag
    10381 acggcgttca ccgacatcgc ggtgctgaag taaggagcgg gtatgatgcg gcgcgggatt
    10441 gccggatgga tgatgtgtct gggcgcggcc ggcctagcgg cgggcggggc cgcagcggtg
    10501 gagctgactc cctcgccgct ggagcaggcg caggtcgagg tacgctacgg cctggcggcg
    10561 gcggggaatc tcgaccggtg ggccgcggcc gaactcgctc ccggggatac gggacaggcg
    10621 ggaacgctcc cgggcgagaa atcgccggtc aaggcgatgc ttctgtcgct ggcggtcccg
    10681 ggcgcgggac aatggtacta cggcagccgg atcaaaccgt tcgtgttcct cggtctcgag
    10741 gcgctcgcct ggggcctcca tgcgcactac cactataacg gcgaggacat gaccgatgaa
    10801 ttcgaggcgt tcaaccggga gcactggtcg cagcaggcgt atgcggacta tctcaactgg
    10861 gcctatgacg gactgacgat aaaggacctc gaggacacag tgattcccgg cctgacgcat
    10921 catttgccgg acacccgaac gcaacagtac tacgagatga cggggaagta caaccagttt
    10981 gcctggggat gggatgacgc gaatctgcag gggctgatgc tgtacgccga ttatagtcag
    11041 agcaatcctc cgcccaagat agaccaggga gtgccggcat cgccccgccg ggacaaatac
    11101 gaggatatgc gaggggacgc caaccacgag ttcaagaagg cgggccggat gctggtgggg
    11161 gtggtggtca accacgtggt gagcggcttg gaggcgttct tcatgacgcg gcaccggaac
    11221 cggcagctgc gcggcgacga cgaggcggag gagttcctgt cgaatctgag catcgagcca
    11281 tcgctgcggt cggtctacag gttccaggac acgccctacg tgcaggtgaa ttactcgttc
    11341 tgaaaaaagg gacagcatga gaattcgggt tgcgatagca ttgggaatcg gtctggtggc
    11401 ggggggggcg gtctgcgcgg ccgcggtcgg ggcggcgggg gtacggccgg cgcccgtgca
    11461 gacaagtctc gccacgcaga tccgggcgga cggactcgcc tggcggccga ccgcggcggt
    11521 caccgaatgg gaggacgacg ggactctgac gccggcgccg aaggagaacc ggccgcagga
    11581 ggggaagaag aagtcgccgg tcaaggcggc ggccctgtcg gcgctcgtgc cggggctggg
    11641 gcagctctac aacgaccgcg acatcaaagc gcgctgcttc ttcgccgccg agggactcac
    11701 ctgggtcgct ttcggggcgt tccggctcta cggcgactgg aagaaggacg acctgatcgc
    11761 ctacgcggcc gtccatgcca acgcgcaatt ggaggggaag agcgaccagt atctcgactg
    11821 ggtggggttc tacgacagca ttgacgattt caacagggcg gggcgggtgg gggacccgga
    11881 ccggcgctac ctgccggatg tgccggaata ccactgggtg tggcccagcg acgccgagcg
    11941 ggagaccttc cgcagcctga agaattcgag ccgggaggca taccggcggg gggactggat
    12001 gctgtgggcg gccgtgatca accgggtggt ggcggtgatc gacgcggtgc tggatgcgcg
    12061 ccgggccaac cgggtggtcg acgagggctg gacgatcggg ggagtgcgct atgatctgga
    12121 attccatccg acatccccga cccgccaggt ggcgctcacg gtgtacccgg ggttttaggc
    12181 ggagtatgcg ggcaggcgcg gtcagcgtag gtgcagcggc ggcggctatc gcgctctcgt
    12241 gcggggggcc gggaggggcc ggggatgaaa ccgcggcccc gccggcgccg gcgggagtga
    12301 ttgtcgagca ggtgatcgcc ggagaggtgc tcggcctccc gctgcagagc ccctggggac
    12361 tggcggcggg aacggacgac aatgtctact gcgtcgaccg ggggaacaac cgcatcatca
    12421 aattcagccg ggctcttcaa ccgatccgcg atgcgggcgg ggcggggagc gaggcgggtc
    12481 tgctcagccg tccgggttac gcagcggtcg acaaccagct caatgtgtgg gtggccgacg
    12541 aggggaacca gcggatctcg cgcttcaatt cggacctcct gttcgtggac gcgaccgagt
    12601 tctacaatgc cgacgacccg ctgcaattcg gggagccggg cggggtggcg gtcagcgcgc
    12661 acggcgagct gtgggcggga gaccgccagc gca
//
LOCUS       DAOPOE010000088        12461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5889_length_12461_cov_5.153232, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000088 DAOPOE010000000
VERSION     DAOPOE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_12461_cov_5.153232"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /gene="uvrA"
                     /locus_tag="PKM94_10860"
     CDS             complement(<1..2323)
                     /gene="uvrA"
                     /locus_tag="PKM94_10860"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD67173.1"
                     /translation="MATSVKPDNRTRINNKARNKRNQEYLHVKGAREHNLQNIDVKIP
                     RNTLTVITGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLS
                     PAISIEQRSAAKNPRSTVGTVTEIYDYLRLLFARVGVPHCVSCGQPISQQTVEQIVDS
                     VLGFEEGTRLMVLAPLVRGKKGEHKDVIDEARGEGFVRLRINGEIVESEADISLDKNK
                     KHTIEAVVDRLVVKESSRTRLADSVETALKVGQGTVLINIKGQDLLYSEQFACLNCNI
                     SYEEPTPRLFSFNSPFGACPVCDGLGQKMEIDPRLVIPDPSLSISDGAIRPWGGHDLA
                     NWYRYMLKGLARHFDFKLSTPFQKLPEKVRKVVLYGSGREEIEFSYEHMTGRGHGGGI
                     YRAPFEGVIPHLERRYRQTESNNVRMWIENFMSVSDCPACRGARLKPEALAVILDRET
                     IDSITSMSIKQAAAFFRRITLTKRQETIARQILKEIRERLQFLCDVGLDYLTLNRAAQ
                     TLSGGEAQRIRLATQIGSRLVGVLYILDEPSIGLHQRDNRKLLATLTELRDIGNTVLV
                     VEHDRETIEAADHIIDLGPRAGIHGGQVVAQGTLRDITRSRESLTGQYLKGERKIEIP
                     GQRRRPNGKTIALTGCRGNNLKAADVEFPLGLFIAVTGVSGSGKSTLVNETLYPILAR
                     HFFGSRRPPLPYGAIAGLEHIDKVIDIDQSPIGRTPRSNPATYTGVFTHIRDLFAAIP
                     ESKARGYQPGRFSFNVKGGRCEACEGDGIIKIEM"
     gene            2518..3123
                     /locus_tag="PKM94_10865"
     CDS             2518..3123
                     /locus_tag="PKM94_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD67174.1"
                     /translation="MSHDKHQEFFDRLAAEWDLMFTAVDLEFLQHIVDQLDIRQGWDI
                     LDLGCGTGVLFDMLRRRVGETGSVTGVDFSFQMAQRANLNFPFPNVAVVDADAQMLPF
                     RDSAFDMAVAFSAFPHFSDQQKALLETHRVLKPRARFYIIHLESSRDLNQLHQRIGGA
                     VASDLIPPGERLREMFSTSKFVEVTIEDHPGLYLATAMNLK"
     gene            3161..3694
                     /locus_tag="PKM94_10870"
     CDS             3161..3694
                     /locus_tag="PKM94_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOD67175.1"
                     /translation="MAHSALYLALAVLLPIGFHALGVAGRIFLPMHFPILLAGFLAGP
                     AAGVVVGLLAPGLSHLLTGMPPTYAVPLMSLELPMYGLAAGIAYRRLHLNIYIALVAA
                     MIVGRLMFGLGLLVLGLFMNLPYNAAQFFSAGGAIVTGLPGMAVQILLIPLIVAAVRR
                     ERTRPAPGEAAAAPDKP"
     gene            complement(3707..4264)
                     /locus_tag="PKM94_10875"
     CDS             complement(3707..4264)
                     /locus_tag="PKM94_10875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD67176.1"
                     /translation="MYVTPAMVEAMAARYGRPGETAFTFPVSAREHAFIRSSQRFQRE
                     HDATMYIVKDGRIVVIAKHHYPPGLYRAPSGGLHPGESFEEGIAREAMEETGAALALE
                     RFLLISRVTFTHTDGDIPWRSYVFQARFESGDFRFTDTREIREVRLAQPAEFETFGRI
                     MRSLEVGGLHYRAALHEAVAPLLIL"
     gene            complement(4303..5478)
                     /locus_tag="PKM94_10880"
     CDS             complement(4303..5478)
                     /locus_tag="PKM94_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD67177.1"
                     /translation="MDIEAIQTYLGNHELDGWLLADFHGRNTIAIEMLGLKGMITRRS
                     FYFIPAIGEPTALVHAIEQDRFAALPGRKIVFSGYKALEKELARVLSSVSRVAMEYAP
                     KGRLPYIGLVDAGTVELVRDLGIEVLSSANLVAHFQAALGVEQIATHRMAARNLLEIK
                     EKTLAHIRASLAEGTPLTEYDVVQFMLRQFRAYDMTTDSSPICAVHANAGNPHYEPTP
                     EHCAPVRKGQLILLDLWAKLNREDGVYGDITWMAFAGTAAEIPQTYRDMFAVLAEARD
                     AAVDYVRRNIETKPVRGADVDDVCRGVIDKAGYGQYFTHRTGHSITSSEHGPGPNIDN
                     LETEDSRKLRKGHLFSIEPGIYTDACGLRTEIDVLIGHEGAEVTTLPLQKEIIPLFS"
     gene            5623..6177
                     /locus_tag="PKM94_10885"
     CDS             5623..6177
                     /locus_tag="PKM94_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD67178.1"
                     /translation="MSEPVRIVITGAPASGKTKFFERLRGHPSLSDFLFFEELARRLL
                     VEDAGYRERPADFHREIYRRQRAREDAAPRRSFITDRGTADAFAFHPETAAEVGTTPA
                     AEYRRYTGVIQLGTAAALGPEYYQADEVRIEPIAAVLALERQIRSVWERHPGYQFVPA
                     RESVDEKYAVFVEIVCRLAGKTPV"
     gene            6247..8490
                     /locus_tag="PKM94_10890"
     CDS             6247..8490
                     /locus_tag="PKM94_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67179.1"
                     /translation="MRLPLVMAALLLWSGAPAADREFFKEFESTYDLLHVELLNNAGE
                     KAEVSNFVYQKDVATFTFEKGTVYLQRYVKDRPTTAVFVGQGRVTIDVPVHSERQGLW
                     AVTKDSTVDQPVEVVAIRMADDLDLRLRERFQFEPAVIDFRVVNKLGQGEFFFKPVIM
                     HTYDNYFRLAASIYERGPDGFFYADFNRYTFTFDPNWPEQVQIGYEVEGGDQVSTVGA
                     SFQRREAGRYDDSLLSQIAYQTTALRRTAEVRLSGTDGRRIEQAKATLDLAVNADSLR
                     FTWLFLKYSLDVDSVLVGDERIDYVRRKTFDYTGIILPRYYHQGDTLSLTVWYRGTNF
                     DHFLPYLSNPVPSPTEVTFNVPKGYNYYTAGMSEPVDVGGGRQEFTSALPAPLGELYF
                     HCFPTGVEDTVQVISDVGVALNFVIADRDLRRDPCYIPRDQYQEAVTAAFNYLSKNFG
                     PPPQTFAEYVISTGFQSAPGMIKVPQVACVTSDPWQVVGGFDGPVGNSVAHQWFGPLM
                     RPQSGREDWLSWALPAYQGLRFIESHRSSAPYFSNLLTRRDSLYTQIQMGHEIPLSAG
                     YRVNDYWDANGKFDFYRTILNNKGVWLLHMLRFLMYDYEAGSDAKFAGFSRELFLQIT
                     NRTFSNADFVRLAEKHYGAPLDWFFRQWLYGAYLPEYNATWTKVQRDGSWYVAVTVQT
                     GKVPPSFQMPVIVRVVEGDGRDAFSRPVITAPSAEFELGPFSQEPRDFVFNEFLSTLG
                     WETVSRK"
     gene            complement(8722..9237)
                     /locus_tag="PKM94_10895"
     CDS             complement(8722..9237)
                     /locus_tag="PKM94_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67180.1"
                     /translation="MSQPSPTCPGSAPQRFTPAADRPQAVLPKVDNNTTLGARPEPQS
                     HLGQWPVQLMLVPTHAGYFTGADLALTADCVPFAFADFHERFLKGRPLAVGCPKLDNA
                     HVYIDKLAEIFALHNLKSMEVIVMEVPCCSGLVQIALAARDQAGVRTPIRVTTIGIRG
                     EQLGTEEIAGR"
     gene            9406..9603
                     /locus_tag="PKM94_10900"
     CDS             9406..9603
                     /locus_tag="PKM94_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD67181.1"
                     /translation="MVTRCACFNQPFTRLKRIARRRRARTIEELQQHVRFGLNCRRCH
                     PYVKLMLATGRTAFEVIAGED"
     gene            9686..12358
                     /locus_tag="PKM94_10905"
     CDS             9686..12358
                     /locus_tag="PKM94_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67182.1"
                     /translation="MTDGHRLLCRRALAALGIVLTIATAHGQSDTTRQVRPRGPRQTA
                     AVNRIYLTAVHRDNNLALAVTNRGFIGAVEYNNEPPRDAFTGWDIRTSCEFPAQSWRI
                     YLEGLELWIGGRTRYGLRLSGGSQAGVFLPSNPPGEIIARSTTDRHGGPHPDAVSEED
                     FICEYTDTLMYREGAGGDEDIVRNPEPLNVKVTQESYAWTYESVRDVVFFNLRVENIG
                     RDGLREVYIGLHVIPTARYNNTRPCNDSGEVVGFFETYEAPIGCGFRDTLNLAWHADR
                     DGNAYGGVFYDRPVGTQVDIADTMCMLASVRSGLGIVPLRGPVDRGGVYYNWWTSRWA
                     PSLGPIFMEPSADPSVRSFGRGSWWYNQDWFYSLESEYRRLSTHEQDHPNYWTGEIAR
                     ALTPPWNPVPLTEANAIASNTTPDYLVSWGPLTLAAGHKVTLSFAVVGGELIHKDPWI
                     LAYIPRRPAEYEQRLDFSDLIANARWAQRVFDNPGFDTDGDGYAGEFRVCVQDSALVG
                     GQWVAQKADTQWYKGDGIPDFRVAYPPPPPPFWLTPVPGGVHVRFNGQVSETQVDPLL
                     QAPDFEGYRIYLGRDERAEALTVVGSFDRRNYDKWVKKPDGWGGHIWDLEGMPYSQEE
                     LRCLYAFGPDPCHDTSFHPEDYTILSNYRHPLFPNDSIFRFEPHGYNASEFDTTCGSD
                     TLCTAVRKVYPEMPDPRTLPVDSITPEMFTAEGYFKFYEYEFTIEHLLPTVAYWVNVT
                     AYDVGSPGAGVYPLESDKTAGLKIVYPTTVWDSPLGQNGKVYVYPNPYRTDAGYREQG
                     LEGRNQNDRWDERVRAVWFANLPPVCVIKVFTLDGDLVRELRHEYAGDDPEGKRAKWG
                     LINKNQQLVETGLYYWTVETPDGKVQMGKLAVLR"
BASE COUNT         2064 a         3740 c         4344 g         2313 t
ORIGIN      
        1 gcatttcgat cttgatgatg ccgtccccct cgcaggcctc gcaccgcccc cccttgacgt
       61 tgaacgagaa ccgtccgggc tgatacccgc gggccttgga ctcggggatg gcggcgaaga
      121 ggtcgcggat gtgggtgaac acgccggtgt aggtggccgg gttggagcgg ggggtgcggc
      181 cgatcgggga ctggtcgatg tcgatgactt tatcgatgtg ttcgagcccg gcgatggcgc
      241 cgtacggcag gggcgggcgc cgagagccga aaaagtgccg ggcgaggatc gggtagagcg
      301 tctcgttcac cagggttgat ttgccggagc cggagacgcc ggtgacggcg atgaagaggc
      361 cgagggggaa ctcgacgtcg gccgctttca ggttgttgcc gcggcagccg gtgagggcga
      421 tggttttccc gttggggcgg cggcgctggc cggggatttc aatcttccgt tctcccttga
      481 ggtactggcc ggtgagcgac tcgcgcgacc gcgtgatgtc gcggagggta ccctgggcga
      541 ccacctgccc gccgtgaatg ccggcgcggg ggccgaggtc gatgatgtgg tcggcggcct
      601 cgatggtctc gcggtcgtgc tcgaccacca ggacggtgtt gccgatgtcg cgcagctcgg
      661 tcagggtggc gaggagtttg cggttgtcgc gctggtgcag gccgatcgag ggctcgtcga
      721 ggatgtagag cacgccgacc aggcgcgagc cgatctgggt ggcgagccgg atgcgctggg
      781 cctcgccgcc cgagagcgtc tgggccgccc ggttcaaagt caggtaatcg aggcccacgt
      841 cgcacaggaa ctggagccgc tcgcggatct ctttgaggat ctggcgggcg atcgtctcct
      901 ggcgcttggt cagggtgatg cggcggaaga aggcggccgc ctgcttgatc gacatggacg
      961 tgatgctgtc gatcgtctcg cggtcgagga tgacggcgag cgcctccggc ttgaggcggg
     1021 cgccgcggca ggccgggcag tcgctcaccg acatgaaatt ctcgatccac atccggacgt
     1081 tgttcgattc ggtctggcgg tagcggcgtt cgaggtgcgg aatgacgcct tcgaagggcg
     1141 cccggtagat gccgccgccg tgcccgcggc ccgtcatgtg ctcgtacgag aattcgattt
     1201 cctcgcgccc ggagccgtag agcaccactt tccggacttt ctcgggcagc ttctggaacg
     1261 gggtggagag tttgaaatcg aagtggcggg cgagcccctt gagcatgtag cggtaccagt
     1321 tggcgagatc gtgcccgccc caggggcgga tcgcgccgtc ggaaatcgaa agcgaagggt
     1381 cggggatcac caaccgcggg tcgatctcca tcttctgccc cagaccgtcg cacaccgggc
     1441 aggcgccgaa cggggaattg aacgagaaca gccggggggt gggctcctcg tagctgatgt
     1501 tgcagttgag gcaggcgaac tgctcggaat agaggaggtc ctgtcccttg atgttgatga
     1561 ggacggttcc ctgccccacc ttgagggccg tctccaccga atcggccaac cgggtgcgcg
     1621 agctttcttt cacgaccagg cggtcgacca ccgcctcgat cgtatgcttc ttgtttttgt
     1681 ccaggctgat gtcggcctcg gattcgacga tctcgccgtt gatgcgcagg cggacaaaac
     1741 cctcgccccg ggcctcgtcg atcacgtcct tgtgctcccc cttcttgccg cggaccagcg
     1801 gcgcgagcac catcaggcgc gtcccctcct cgaaaccgag gacgctgtcg acgatttgct
     1861 cgaccgtctg ctgcgaaatc ggctggccgc aggagacgca gtggggaacg ccgacgcgcg
     1921 cgaagagcag acggaggtag tcgtagattt cggtgacggt gccgacggtg gagcgggggt
     1981 tcttggccgc gctgcgctgt tcgatcgaaa tggccgggga gagtccctcg atgaaatcga
     2041 cgtccggctt ctccatcagg ccgaggaact ggcgggcgta ggcggagagg gactcgacat
     2101 agcgccgctg cccctcggcg tagatggtgt cgaaggcgag ggaggatttg ccggatccgg
     2161 agagcccggt aatgacggtc agggtgttgc gggggatttt gacgtcgata ttctggaggt
     2221 tatgttcgcg cgcccctttg acatgcaggt attcctggtt gcgcttgtta cgggccttgt
     2281 tgttgatccg ggtgcggttg tcgggtttca cgctggtcgc catcggcatc tccgattttg
     2341 tgtcaacccg taatatacta caccggttcc ccgccgccaa cggcgacttc gatttcgcca
     2401 tcacattatg cgggccgccg ctaccagagc ccgcctggcc cggccaagcg caaggacgcc
     2461 ggagcgaggg gaggaaagct ccttgaccgg gcgcgggcgg tgtgctaaat tcgggtcatg
     2521 tcgcacgata agcatcagga attcttcgac cggctggcgg cggaatggga cctcatgttc
     2581 accgcggtgg atcttgagtt cctccagcat attgttgacc agcttgatat taggcagggc
     2641 tgggacatcc tggatctcgg ttgcggcacc ggagtattgt tcgacatgct gcggcgtcgg
     2701 gtgggcgaga ccggatctgt cacaggggtg gatttttcgt ttcagatggc tcagcgggcg
     2761 aaccttaatt tcccctttcc caatgtcgcg gtggttgatg ccgacgcgca gatgctgccg
     2821 tttcgggaca gtgccttcga catggccgtc gcgttttcgg cctttccgca tttctccgac
     2881 cagcagaaag cgctcctgga gacccatcgg gtgctcaagc cgagagcccg tttctacatt
     2941 attcacctgg agtcctcccg ggatttgaac cagctccacc agcggatcgg cggggcggtg
     3001 gcgtccgacc tgattccgcc gggggagcgg ttgcgggaga tgttcagcac gagcaagttt
     3061 gtcgaggtga ccatcgagga ccatccgggc ctgtacctcg cgacggcgat gaatctgaag
     3121 tagtctatcg gtgtcaccaa ctaccgccag gacccgattc gtggcgcact cggcgctcta
     3181 ccttgcgctg gcggtgcttt tgccgatcgg gttccacgcg ctgggggtgg ccgggcggat
     3241 tttcctgccg atgcactttc ccattctgct ggccgggttt ctggccgggc cggcggccgg
     3301 cgtggtggtg gggctgctgg cgccggggtt gtcgcacctg ctcaccggca tgccgccgac
     3361 ctacgcggtg ccgctcatgt cgctggaact gcccatgtac ggtctggcgg cggggattgc
     3421 ctaccgccgg cttcacctga atatctacat cgcgctcgtt gcggcgatga tcgtggggcg
     3481 gctgatgttc gggctggggt tgctcgtgct cggtctgttc atgaatctcc cctacaacgc
     3541 ggcccagttc ttctccgccg gcggggcgat cgtcacgggt ctgccgggga tggccgtcca
     3601 gatactgctg attccgctga tcgtggccgc ggtgcggcgg gagcgcaccc gcccggcgcc
     3661 cggagaagcc gcggccgcgc cggacaagcc gtagggccgc gcactttcag agaatgagca
     3721 gcggggcgac cgcctcgtgc agggccgccc ggtagtgcag gccaccgacc tccagcgagc
     3781 gcatgatgcg gccgaaggtt tcaaattccg ccggttgcgc caggcggacc tcccgaattt
     3841 cgcgggtgtc ggtgaaccgg aaatcgccgc tttcgaagcg ggcctggaac acgtacgacc
     3901 gccagggaat gtcgccatcg gtgtgggtga aggtgacgcg cgatatgagg aggaagcgtt
     3961 ccagcgcgag agccgcgccg gtctcctcca tcgcctcgcg ggcgatcccc tcttcgaacg
     4021 actcgccggg gtgcaggccg ccggaggggg cgcggtacaa accgggcggg tagtggtgtt
     4081 tggcgatcac cacgatccgc ccgtctttga cgatgtacat cgtcgcatcg tgctcgcgct
     4141 ggaaccgctg ggaggagcgg atgaaggcgt gctcgcgggc ggacacggga aaggtgaacg
     4201 cggtttcgcc gggccgcccg tagcgggcgg ccatggcctc gaccatcgcg ggggtgacgt
     4261 acatgcggtt gtccggtcgg cgagttcctt gcggctcgcc ggctacgaaa acagagggat
     4321 aatctccttc tgcagcggca gcgtcgtgac ctcggctccc tcatggccga tgagtacatc
     4381 gatttcggtg cgcaggccgc aggcgtcggt gtagattccc ggctcgatcg agaagaggtg
     4441 tcccttgcgg agcttgcggc tgtcctcggt ttcgagattg tcgatattcg gtcccgggcc
     4501 gtgctcggac gaggtgatcg agtggccggt gcggtgggtg aaatactggc cgtagccggc
     4561 cttgtcgatg accccccggc agacatcgtc gacatccgcg ccgcggaccg gcttggtttc
     4621 gatgttgcgc cgaacgtagt cgacggcggc gtcccgggcc tccgccagca ccgcgaacat
     4681 gtcgcggtag gtctgcggaa tctcggcggc cgttcccgca aaggccatcc aggtgatgtc
     4741 gccgtacacg ccatcctccc ggttcaactt cgcccagaga tcaaggagga tcagctgtcc
     4801 tttccggacg ggggcgcagt gctccggggt cggctcgtag tgggggttgc ccgcgttggc
     4861 gtggacggcg caaatcgggg agctgtcggt ggtcatatca taggcgcgga actgccggag
     4921 catgaactgg acgacgtcgt attcggtcag gggcgttccc tcggccaggc tcgcgcggat
     4981 gtgagcgagc gtcttttcct tgatctccag taggttgcgg gcggccatgc ggtgggtggc
     5041 gatctgctcg acgccgaggg cggcctggaa atgggccacc aggttggccg acgagagcac
     5101 ctcgatcccc aggtcgcgca ccaattcgac ggtaccggcg tcgaccaggc cgatatacgg
     5161 cagccgccct ttgggagcat actccatggc gacccgggag acgctggaga gcacgcgggc
     5221 gagttctttc tccagcgcct tgtagccgga gaagacgatc ttgcggccgg ggagggcggc
     5281 gaagcgatcc tgttcgatgg cgtggacgag cgccgtcggt tctccgatgg cggggatgaa
     5341 gtagaaggag cggcgcgtaa tcatcccctt caggccgagc atctcaatcg cgatggtgtt
     5401 gcggccgtgg aagtcggcca ggagccatcc atcgagttcg tggttgccga ggtaggtttg
     5461 aatagcctcg atgtccatgg gtcagacctt tcgcagcgtg agcggttgtt gggtgccagt
     5521 ataatgcatg gggggtgggg ttgtcgaccg ttatttggcc gccgctcttg acgcgtcggg
     5581 ccgagcgatc taccttgccg gtgacagcgg gggctgtcac gtatgtccga accagtgcgg
     5641 atcgtcatca ccggggcgcc ggcctccggc aagaccaagt ttttcgagcg gctgcgcggg
     5701 cacccctcgc tgagcgattt cctctttttc gaggagctcg cccggcgact gctggtcgag
     5761 gatgccggct accgcgaacg cccggccgat ttccaccgcg agatctatcg ccgccagcgg
     5821 gcccgcgagg acgccgcgcc gagacggtcg ttcatcaccg accgcggcac ggccgacgcc
     5881 ttcgccttcc acccggagac ggctgcggag gtcggcacga cgccggccgc cgagtaccgg
     5941 cggtacaccg gggtcatcca gctcggcacg gccgccgcgc tggggccgga gtactaccag
     6001 gcggacgaag tccgcatcga gccgatcgcg gcggtgctgg ctctcgagcg gcagatccgg
     6061 tcggtctggg agcggcaccc gggttaccaa tttgtcccgg cgcgggaatc ggtggacgaa
     6121 aaatacgcgg ttttcgtgga gatcgtgtgc cggctggccg gaaaaacacc ggtttaattt
     6181 gacagcgggg gggatcctgg cgtttataag atactgaaag acagggacta aaatgtcgag
     6241 ggagctatga gattaccgct cgtgatggcc gctctgctgc tgtggtccgg cgcgcccgcc
     6301 gccgaccggg agttcttcaa ggagttcgaa tccacctacg acctcctcca cgtggagctg
     6361 ctcaataacg ccggcgagaa ggccgaggtc agcaatttcg tctatcagaa agacgtcgcc
     6421 acgttcacat tcgagaaagg gacggtgtat ctccagcggt atgtgaaaga ccgcccgacc
     6481 acggccgtgt ttgtgggcca gggacgcgtc acgatcgacg ttccggtgca ttcggaacga
     6541 caggggctgt gggccgtgac caaggacagc acggtggacc agccggtcga ggtggtggcg
     6601 atccggatgg cggatgatct cgatctgcgg ctgcgggaga gattccagtt tgagccggcg
     6661 gtcattgatt tccgcgtcgt gaacaagctt ggccagggcg agttcttctt caaaccggtg
     6721 atcatgcaca cctacgacaa ttatttccgc ctcgccgcat cgatctatga acggggcccc
     6781 gacgggttct tctatgccga tttcaaccgc tacacgttca cctttgaccc caactggccg
     6841 gagcaggtgc agatcgggta cgaggtggag gggggcgacc aggtgtcgac cgtgggggcg
     6901 agcttccagc gccgggaggc gggccggtac gacgactcgc tgctgtcgca gatcgcgtac
     6961 cagacgacgg cgctccgccg cacggccgag gtgcggctgt cggggaccga cggccgccgc
     7021 atcgagcagg cgaaagcgac gctcgacctg gcggtcaacg ccgacagcct ccgcttcacc
     7081 tggttgttcc tcaaatactc actcgacgtg gattccgtgc tggtcgggga cgagcgcatc
     7141 gactatgtgc ggcggaagac gttcgactac acgggcatta tcctcccccg ctactaccac
     7201 cagggggata cgctcagcct gacggtgtgg taccgcggca cgaacttcga ccacttcctg
     7261 ccctacctga gcaatcccgt tccgagcccg acagaggtga cgttcaacgt ccccaagggc
     7321 tacaactact acacggcggg gatgagcgag ccggtggacg tcgggggcgg gcggcaggag
     7381 ttcacctcgg ctcttccggc gccgctgggc gagctgtact tccactgttt cccgaccggc
     7441 gtggaagaca cggtgcaggt gatatcggac gtgggggtcg cgctcaattt cgtcatcgcc
     7501 gaccgggatc tccggcggga tccctgctac atcccgcgcg atcagtatca ggaggcggtg
     7561 acggcggcgt tcaactacct gtcgaagaat tttggtccgc cgccgcagac gtttgcggag
     7621 tatgtgatat cgaccggttt ccagagcgcg ccggggatga tcaaggtgcc gcaggtggcc
     7681 tgcgtgacga gcgatccctg gcaggtggtg ggggggttcg acggtccggt cgggaacagc
     7741 gtcgcccacc agtggttcgg gccgctcatg cgtccgcagt ccgggcgcga ggattggctc
     7801 tcctgggcgc tcccggccta ccaggggctg cgcttcatcg aatcccaccg ctcctccgcg
     7861 ccgtatttct ccaatctgct gacccgccgc gactccctct acacccagat ccagatgggc
     7921 cacgagatcc cgctgtcggc cggctaccgg gtcaatgatt actgggacgc caacgggaag
     7981 ttcgacttct accggacgat cctgaacaac aaaggggtct ggctgctcca catgctgcgg
     8041 ttcctgatgt atgactacga ggccgggtcg gacgccaagt tcgccggttt ctcgcgcgag
     8101 ctgttcctgc agatcaccaa ccgcacgttc agcaacgccg atttcgtccg cctcgcggag
     8161 aaacactacg gcgcgccgct cgactggttt ttccggcagt ggctctacgg ggcgtacctg
     8221 ccggagtaca acgcgacctg gacaaaagtg cagcgcgacg ggtcgtggta cgtcgcggtg
     8281 acagtgcaga ccgggaaggt tcctccgtca ttccaaatgc cggtgatcgt acgggtggtg
     8341 gaaggggacg gccgggacgc gttctcgcgg ccggtgatca ccgccccgtc ggcggagttc
     8401 gaactcgggc cgttcagcca ggaaccgagg gatttcgtgt tcaacgagtt tctctccacg
     8461 ctcgggtggg agacggtgtc ccggaagtaa ggcgggcccg gctccggccg gggcgggtaa
     8521 gattgggcgc aaaagaaaag cgccgggtca tgcggcccgg cgctctttct tgcacgaacg
     8581 caaagggagg aaagtatagc tttcaggtca ttctgtcttc cgttgtctta gacactccac
     8641 gccgccgaaa gttccggcaa cggcgcgatc gtggatcgaa gatcctgacc gggcggggcg
     8701 gccgccccgc tgtcgcggcg gctagcggcc ggcgatctcc tcggtgccca gttgttcgcc
     8761 gcgaatgccg attgtcgtga cccggatcgg ggtccgcacg ccggcctggt cgcgggcggc
     8821 cagcgcaatc tgcaccaggc cgctgcaaca cggcacttcc atcacgatga cttccattga
     8881 cttgagatta tgcagggcga aaatctcggc gagcttgtcg atgtaaacat gggcgttgtc
     8941 gagcttcggg cagcccaccg ccagcggccg gcctttgagg aaacgctcgt ggaagtcggc
     9001 gaaggcaaag gggacgcagt cggcggtgag cgccaggtcg gccccggtga aatagccggc
     9061 gtgggtgggc acgagcatga gctgcaccgg ccactggccg aggtgcgatt gcggttcggg
     9121 gcgggcgccg agcgtggtgt tgttgtcaac tttcggcagg accgcctgcg gccggtccgc
     9181 ggccggggtg aaccgctgcg gggcgctgcc cgggcaagtg ggcgagggtt gtgacatggt
     9241 caggtctcca tccggtgttg tgggatcatt cataagataa agaaaggggg cagcggcgga
     9301 gggcgatatg acttaagtca atcgcggtcc ccgggcggcg ggggcgtgat cggcgtctcc
     9361 ggtttccgcg gaacccgcgc gcggcgttgc tgttacagcg acaccatggt tacccgctgc
     9421 gcctgcttca atcagccctt tacccgcctc aagcggattg cgcgccgccg ccgcgcgcga
     9481 acgatcgagg agctccagca acacgttcgg ttcgggctga actgccggcg gtgccatccg
     9541 tatgtgaagc tgatgttggc gacgggtcgc acggcgtttg aggtgatcgc cggggaggac
     9601 tgacgcggcg cgcggtctgc ccgcagacgt tttcctgttg cacgatccgg cggggggtgc
     9661 gttattgcgg ccgaggagat gcttcgtgac ggatggacac cgcttgcttt gccggcgggc
     9721 cctggcggcc ctcggcatcg ttttgacgat cgcaacagcg cacgggcaga gcgacacgac
     9781 ccggcaggtt cgaccgcggg ggccaagaca gaccgccgct gtcaaccgga tctacctgac
     9841 cgccgtccac cgcgacaaca acctggccct ggcggtcacc aaccgggggt ttatcggggc
     9901 ggtcgaatac aacaacgaac ctccccggga cgcattcacc ggttgggata tccgcacaag
     9961 ttgtgagttc cccgcccaat cgtggcgcat ttacctcgag ggcctggagt tgtggatcgg
    10021 ggggcggacg cgttacgggt tgcggctgtc ggggggatcg caggccggcg ttttccttcc
    10081 ctccaacccc ccgggggaga ttatcgcccg ctcgaccact gaccggcacg gaggcccgca
    10141 tccggacgca gtctcggagg aggatttcat ctgcgagtac accgacacgc tgatgtaccg
    10201 ggagggggcc ggcggggacg aggatatcgt gcgcaacccc gagccgctga atgtcaaggt
    10261 gacgcaggag tcgtacgcct ggacctacga atcggtgcgg gacgtggtct ttttcaacct
    10321 gcgggtcgag aatatcggcc gcgacgggct tcgggaggta tacatcggtc tgcacgtgat
    10381 cccgaccgcg cgctacaaca acacgcggcc gtgcaatgac tcgggcgagg tggtggggtt
    10441 ttttgagacg tacgaggcgc cgatcggctg cggtttccgg gacacgctca acctagcctg
    10501 gcacgccgac cgcgacggca acgcctacgg gggggtgttc tatgacaggc cggtgggaac
    10561 acaggtggat atcgccgaca ccatgtgcat gctggcctcg gtgcgcagcg gtctggggat
    10621 agtccccctc cgcgggccgg tggaccgcgg gggcgtctac tacaactggt ggacgagccg
    10681 gtgggcgccc agtctcgggc cgatcttcat ggagccctcc gccgatccgt cggtgaggtc
    10741 attcggccgg ggaagctggt ggtacaatca ggactggttc tattcgctcg agtcggagta
    10801 ccggcggctc tccacgcacg aacaggacca cccgaactac tggacggggg agattgcgcg
    10861 cgccctgacg cccccctgga atccggtgcc gctgaccgag gcgaatgcga tcgcctcgaa
    10921 cacgacgccc gactacctgg tctcgtgggg accgctgacg ctggcggccg ggcacaaggt
    10981 gacgctctcg tttgccgtgg tcggcggaga actgatccac aaggatccgt ggatcctcgc
    11041 ctacatcccg cggcggccgg ccgagtacga acaacggctg gatttctcgg acctgatcgc
    11101 gaacgcccgc tgggcccagc gcgtgttcga caaccccggt ttcgacacgg atggcgacgg
    11161 gtatgccggc gaattccgcg tctgtgtcca ggattccgcc ctggtgggcg gccagtgggt
    11221 cgcccagaaa gccgacaccc agtggtataa gggggacggc atcccggatt tccgggtcgc
    11281 ctacccgcct ccgccccccc cgttctggct cacccccgtc ccgggcggcg tccatgtccg
    11341 gttcaacggc caggtgtccg agacccaggt cgatccgctc ctgcaggcgc cggatttcga
    11401 gggctatcgc atctacctcg gccgcgacga gcgagcggaa gcgctcaccg tggtcggctc
    11461 gttcgaccgg cgcaactacg acaagtgggt gaagaagcct gatggctggg ggggacacat
    11521 ctgggacctc gaggggatgc cgtactcgca ggaggaactc cgctgcctct acgccttcgg
    11581 ccccgacccc tgtcacgaca cgagtttcca ccccgaggac tacacaatcc tgtcgaatta
    11641 ccgccacccg ctcttcccca acgattccat tttccggttt gagccccacg gctacaacgc
    11701 ctcggagttc gacactacct gcgggagcga cacgctctgc actgccgtgc ggaaagtgta
    11761 tccggagatg cccgatccgc ggacgttgcc ggtggattcg atcactccgg agatgttcac
    11821 ggccgagggg tatttcaagt tttacgagta cgagttcacg atcgaacact tgctgccgac
    11881 cgtggcgtac tgggtgaatg tgacggcgta cgatgtcggg agtcccggcg cgggggtcta
    11941 cccgctggag tccgacaaga cggcggggct gaagatcgtc tacccgacca ctgtctggga
    12001 tagcccgctc gggcagaacg gcaaagtgta tgtgtacccg aatccgtacc ggaccgatgc
    12061 gggctatcgg gagcagggac tggaggggcg gaaccagaac gaccggtggg atgagcgggt
    12121 gcgggcggtg tggtttgcga acctgccgcc ggtctgcgtg atcaaagtgt ttacgctcga
    12181 tggcgacctg gtgcgcgagc tgaggcatga atacgcgggg gacgatccgg aggggaaacg
    12241 ggccaagtgg gggctgatca acaagaacca gcagttggtg gagaccgggt tgtattactg
    12301 gacggtggag acgccggacg gcaaggtgca gatggggaag ctcgccgtcc tccggtaacc
    12361 gcgtccgcgg ggaccaacca tcggacaggc cggcttgtat aagcggcggg acccggctat
    12421 cttgaacggg ccagcgagcc gggtcgtgac gccgggccga g
//
LOCUS       DAOPOE010000089        12291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_5966_length_12291_cov_5.716574, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000089 DAOPOE010000000
VERSION     DAOPOE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_12291_cov_5.716574"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PKM94_10910"
     CDS             <1..1212
                     /locus_tag="PKM94_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67183.1"
                     /translation="GAYLNPERRIRSYHALPPGESTTIEAAVLVPGNFGQAQVYLRLY
                     DVVDTLDEILPGQKFYEQPFLVTFHVPEGLRPYVDTRVTLPPRVEDHPYFDNEFARLL
                     FLLLGEGKLPAEIAALAECDTGFVSEQLALMVGEGYVGKDSTGFKLKIPVIRAAEADS
                     ARSRALVLADSLTDLIAANLPRFAAVRDSLVAAGRVNRDSNAFFDPGTVLYHTYPVVG
                     GLALWWDLGNAFITRSAPLYLYDGTDLCNADIPIYMYAVEGGPVVNGHLFYAPVVSST
                     SYELYFGDTIPTIACDPDFIRFKSYPIHVNFQPAEEYRPTTYVFDSVVVRPAVEALTY
                     GADSLLAHAYLDLRDMAVDKFGHPRLDYGYRYWFWNLVATRTLEGLVARGAVRRQGNG
                     QFRLTATEFGG"
     gene            1214..1660
                     /locus_tag="PKM94_10915"
     CDS             1214..1660
                     /locus_tag="PKM94_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67184.1"
                     /translation="MRTPVLLCALALAAAAGCSGEKTPPRDQIPALREAVYQLQERVK
                     AHHLAALDSLLSVKILDRRQSGDSLLSFVYGPERSFAFEQFGGCEIAYTRDHAVAECW
                     IMDTTHARTRPIRLEFARDGDRWLLASFGTGAAAVDSTPPDSAARP"
     gene            1720..2607
                     /locus_tag="PKM94_10920"
     CDS             1720..2607
                     /locus_tag="PKM94_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOD67185.1"
                     /translation="MKRHFNSPCCAAFFVALILCVFAPLASSSAPAANLSDCAAPPER
                     PTLLLYAFEAEGALLESRMAVDSAAAHLGRRVVYGRLSDRPVVLAESGVGMTNAAMTA
                     QALLDRCRPARVLFSGIAGGIDSSVHIGDIVVCSAWVAHEYGYWGAEGFAPDPVEVYL
                     PVLDTAVSLNALSVDVDLFRTAGRLAGDTFAFDSIGGRQPRVRVGGVGASGNSFVDNV
                     EKRLWLSATFSAQVVDMESAAVVQVCTANGVPCLVFRSASDLAGGSGSSTARTEIGEF
                     FRVAAANSAQVVLRFLEHL"
     gene            complement(2930..3529)
                     /gene="trmB"
                     /locus_tag="PKM94_10925"
     CDS             complement(2930..3529)
                     /gene="trmB"
                     /locus_tag="PKM94_10925"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOD67186.1"
                     /translation="MKEFPWAWEERKEILVYPEGSAVGEGDILEIGPGRGDLLLSLAE
                     ALPEKQFVAVEIGKKRYYKLIPRIQKRSLANIQLICSDARIVLPRFYGRPAFEKIYVL
                     FPDPWPKARHEFRRLLHKSFVELLAAVLRPDGDLFMATDVDWYAQWAVENAAAVAGLR
                     SLGRPFVAAEALFSYAPTFFEQKWRAEGRPIYYMHYRRC"
     gene            complement(3645..4550)
                     /locus_tag="PKM94_10930"
     CDS             complement(3645..4550)
                     /locus_tag="PKM94_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOD67187.1"
                     /translation="MKVAVAGASGLIGSAVVPHLQSRGHLVYRLVRRPVRDAAAEIEW
                     HPETGAVEPVALAGFDVIINLAGENIGEGRWSEERKRRIRDSRVETTRLLAGLCAALD
                     PQPKAFLSASATGYYGDTGERAVDESAQAGDTFLADVCRQWEEAAQPAAEAGIRVVNF
                     RLGVVLSRQGGMLHKILPLFRLGLGGRLGDGRQYLPWVTLEDVARAFEYFITNEPIRG
                     PVNVVAPQPVTNAEFTQALGAALQRPTLFRVPAGVIRAVFGQMGRETILSGCRAVPAR
                     LSTQGFRFKHADLVDTLRAELDRPG"
     gene            complement(4667..4906)
                     /locus_tag="PKM94_10935"
     CDS             complement(4667..4906)
                     /locus_tag="PKM94_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOD67188.1"
                     /translation="MSHPNAGNPPARTAKRIAIGAGALLALILIVQNTAVVTFRFLVW
                     QMSISQVLLIPLVLALGFAIGMLAYPLLFRRRTKG"
     gene            complement(5072..7267)
                     /gene="rpsA"
                     /locus_tag="PKM94_10940"
     CDS             complement(5072..7267)
                     /gene="rpsA"
                     /locus_tag="PKM94_10940"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOD67189.1"
                     /translation="MATAKQSETATQRLSKRKAQSTRKTKRVKTKAARPEGIDLEQQG
                     LSAEDQQQRERVVTSRHKRVAEKAQQRLSRTEEGLDDGSSAVQAVRITDIAGVVYDPD
                     EYQQMVDMYDQTIKDIKEGEVVAGRVIGINRDDVIVDVGFKSEGIIAMGEFQQPVTVR
                     MGDEIEVYLEQIEDAHGQLILSKQKADFMRVWEKIREVHDSGEKISGIVARRIKGGLV
                     VDIMGVDAFLPGSQVALRQVPDFDALIGQSMDVKIIKINKARRNIVVSRRIVLEEERE
                     KMRAHLLNEIAVGQVRQGIVKNITDFGVFIDLGGVDGLLHITDMSWGRIRHPSELVSL
                     GDRIDVKILDFDERTSRISLGLKQMTPYPWENIEQKYPLGRKVKGRVVSITDYGAFVE
                     LEKGIEGLIHISEMSWTQHIKHPSKIMNVNDEVEAMVLSVDKENEKISLGIKQMEPDP
                     WTTIEMKYPPGRIVTGKVRNLTAFGAFVELEEGIDGLVHISDMSWTKRIQHPSEVMKK
                     GDRVDVKILKIDHENRRISLGYKQLEDDPWPEIARKYGVGAEVLGTIQRVLDRGVVVE
                     LDGAVEGFVPATQLGRKDLTDPTGVFKEGDEIPLQVIEFDRNQHKIVLSVSAYYKKRE
                     RTELDNFLATHPVANAGPSMAEAIPEELRRQAEAAPEPRDERSHDRTHDRDADRDDYP
                     RPAPPREETTSMAEAMPEELRRMASQANQENPEPPDAGTSPEEGGERPE"
     gene            complement(7558..8172)
                     /locus_tag="PKM94_10945"
     CDS             complement(7558..8172)
                     /locus_tag="PKM94_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD67190.1"
                     /translation="MKILYWTGYFIAKIAGRVIFRGRIIGKEHVPRSGGFILASNHRS
                     YFDPPLAGSATGREMAFFAKAELFAVPVLGSLIRRTNAMPVKRGSVDRDALKTAVATI
                     RKGYGLTVFPEGTRSRDDSFLEPKPGLGLIALHAECPIVPCYLHGTNKLKQCLLGREK
                     LRVAFGEPLPVEWVRSFPKSKEGYVQLAREVMDRIAKIKAASGL"
     gene            8452..9051
                     /locus_tag="PKM94_10950"
     CDS             8452..9051
                     /locus_tag="PKM94_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67191.1"
                     /translation="MFRLLICSGAVLAIAGAVAAAESPIDEGSLYLDGSVFFQRQSGE
                     LWGDGLTSAGVGNGSFSLQEGFEIGPTVGYFTSPGLFVGGQFAILYYEQGKSNLAAIA
                     LGPTVGYFFRTGQGRTEVKGSAYPYIRGFLSWGNLVSGGDVAIWQFGGKGGLLYMLSG
                     AVALDIAARFQGDSWRPDGGLSINGTTVSVGIGVSAFIY"
     gene            9185..9526
                     /locus_tag="PKM94_10955"
     CDS             9185..9526
                     /locus_tag="PKM94_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOD67192.1"
                     /translation="MNHFPIPAITRTSASAEELAVYRALKPYLARCLRLNHDVNNALA
                     GIIGYTDFLLEGGSVLTEDQRRFLSQIMQAAEKVRRLVEELCDEKVAAAQSIDLAALS
                     RALEQMENDSD"
     gene            complement(9518..11686)
                     /locus_tag="PKM94_10960"
     CDS             complement(9518..11686)
                     /locus_tag="PKM94_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67193.1"
                     /translation="MFGFHDLSINVLTNSPVIVALALAALVGLAVHLYRRTNPPLPAY
                     LRAILGGLRIGALLLVFLALLEPVVSYTREYVRRPRVAVLVDQSLSMNKTEDGRSRRA
                     RTDSLLASATLDNLRRVADLAPFFFGGSLGAARGSVDSTSTALGDALFELDKAQTAEP
                     SDYWLLLSDGRSNAGRKPLDVAPSLRSPVISVDVSGGAESFDAGIDEVAFNPVLFVGR
                     PTEIRVKLTWRNAEGRTLDVRLVDSARTAAESRLTVTQAAGRGEVMLRYTPEAPGQRL
                     LQIALPPGENEESAANNQRTISVKVLKSRLSVLLAADRPDYEVGFLKRLLDGSDRYEV
                     TLLATGARAGNLAGRFPTVQTELNRYDLVILYDPDPAAYTSRQEIIRSYLREKGGAVW
                     LFMGPQFAARGPTSWLNDLLPFCQRIAQPLQYVEGRAEPLESHLFHPAIRLADNQAAI
                     REAWASLPPFESLVWCDSIAPDGVVLAEFSRRTPAYAPPALGFRRLGPGKLFASAVLP
                     FWPWGFVNLGFGEDDSNYRKFVDGVVGWLTVKDDFDPVRVQPEKDVFTRGEPVLFNGF
                     AFDLGYRPIPGATGMVEVRGGPDDEEYQRDLVDEGEGRFRAVFENLAPGRYRYAAAIV
                     KDGELLRADSGSFLIEAFSLEEFDQSGDPDLLAALGRTTGGRYYSYRQFDRILDDLDL
                     AEIPVSTRREFTLWNKVWMLLAAIGALSLEWLLRKANQLV"
     gene            complement(11763..>12291)
                     /locus_tag="PKM94_10965"
     CDS             complement(11763..>12291)
                     /locus_tag="PKM94_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD67194.1"
                     /translation="QIEETGNYALSEEEYLTIIADKTASLFSLACETGPILAGRGRRD
                     RQRFAHFGEKIGTAFQIVDDLLDFVGDAEITGKEPGNDVLTGKVTLPLIHALKKAGRG
                     GHDEIIAHLRRRDRRPSFERVLAFVREQGGIEHAYGRARALSEEGLAAITPVTPSEYF
                     DCLVSLVQFTISRAS"
BASE COUNT         1964 a         4045 c         4051 g         2231 t
ORIGIN      
        1 ggggcctacc tcaaccccga gcgccgtatc cgctcctacc atgccctgcc gcccggcgaa
       61 tccacgacga tcgaggccgc cgtcctggtc cccggcaact tcggccaggc ccaggtgtac
      121 cttcgtctct acgacgtggt cgacactctc gatgaaatcc tgcccggcca gaaattctac
      181 gaacagccct tcctggtcac tttccacgta cccgaggggc tgcggccgta tgtcgacacc
      241 cgcgtcaccc ttccgccccg ggtcgaggac cacccctact tcgacaacga attcgcccgc
      301 ctcctgttcc tcctcctggg cgagggcaaa ctcccggcgg agatcgccgc gctcgccgag
      361 tgcgacaccg gattcgtcag tgagcagctc gccctgatgg tgggcgaggg gtatgtcgga
      421 aaggactcga ccggattcaa gctcaaaatt ccggtcattc gcgccgccga ggccgactcg
      481 gcccgctccc gcgctctggt cctggccgac tctctgaccg atctcatcgc cgccaacctc
      541 ccgcgctttg cggctgtccg cgacagtctc gtcgccgccg gccgcgtcaa ccgcgacagc
      601 aacgccttct tcgaccccgg caccgtcctc taccacacct accccgtggt cggcggtctc
      661 gccctctggt gggatctcgg caacgctttc atcacccgct ccgcgccgct ctacctctac
      721 gacggcaccg acctctgcaa cgccgacatt cccatttaca tgtatgcggt cgagggcggc
      781 ccggtggtca acggccacct cttttacgcc ccggtcgttt cgagtacgag ctacgagctc
      841 tacttcggcg acaccattcc cacgatcgcc tgcgaccccg acttcatccg cttcaagtcc
      901 tacccgatcc acgtcaactt ccagcccgcc gaggagtacc gtccgaccac ctatgttttc
      961 gacagcgtgg tcgttcgccc ggcggtcgag gcgctcacgt acggggccga ttcgctcttg
     1021 gcgcacgcgt accttgatct tcgcgacatg gcggtcgata aattcggcca cccccggctc
     1081 gattacggct accggtactg gttctggaac ctggttgcga cgcgcacgtt ggaagggctc
     1141 gtcgcgcgcg gcgccgtgcg tcgccaaggc aacggtcagt tccggctcac cgccacagaa
     1201 ttcggaggct gacatgcgca cccccgtgct cctctgcgcg ctcgccctgg ccgccgcggc
     1261 cggctgcagc ggcgaaaaga cgcccccccg cgatcagatc cccgcgctga gggaagccgt
     1321 ctatcaactc caggaacggg tcaaggcaca ccatctcgcc gcccttgatt cgcttttgtc
     1381 ggtgaaaatc ctcgaccggc ggcagtcggg cgattcgctt ctgagtttcg tctatggacc
     1441 ggagcgcagt ttcgcgtttg aacaattcgg cggatgcgag atcgcgtaca ccagggacca
     1501 cgccgtcgcc gagtgctgga tcatggacac cacgcacgcc cgcacccgcc ctatccggct
     1561 ggagttcgcc cgcgacggcg accgctggct gctcgcgtcg ttcggcacgg gggcggcggc
     1621 cgttgattcg accccgcccg acagcgccgc ccgaccgtag cggggaccga tcaccgtcct
     1681 tcattggact gaaacctctc gcgccaggtg gagggattca tgaagcgtca tttcaactca
     1741 ccatgctgtg cggcgttttt cgttgcgctc attctctgtg ttttcgctcc ccttgcatct
     1801 tcttctgctc ccgcagcgaa tctatccgat tgcgccgccc cgcccgagcg ccccaccctg
     1861 ctgctatatg cgttcgaggc cgagggagcc cttctggaga gccgcatggc ggtcgacagc
     1921 gccgccgccc acctcgggcg gcgggtcgtc tatggccggc tgtcggatcg gccggtggtg
     1981 ctcgcggagt cgggggtcgg gatgaccaac gcggcgatga ccgcacaggc gctcctcgac
     2041 cgctgccgcc ccgcgcgcgt cctcttctcc ggcatcgccg gggggatcga ttcgagcgtc
     2101 cacatcggcg acatcgtcgt ctgctcggcc tgggtcgctc acgagtacgg gtactggggc
     2161 gccgaggggt ttgcgcccga cccggtcgaa gtgtacctgc ccgtgctcga tactgccgtg
     2221 tccctgaatg cgctctctgt cgacgtggac ctcttcagga cggccgggcg gttggccggc
     2281 gatacgttcg cgtttgattc gatcgggggg cgtcagccgc gcgtgcgcgt cggcggggtc
     2341 ggcgcgagcg gcaattcgtt tgtcgacaac gtggaaaaac gcctctggtt gtccgcgact
     2401 ttcagcgcgc aggtggttga catggagtcg gccgcggtgg tgcaggtgtg cacggccaac
     2461 ggcgtcccgt gcctggtctt ccggtcggcc tcggacctgg ccggcgggtc gggttcgtcg
     2521 accgcccgca ccgagatcgg cgaattcttc cgggtggccg ccgccaacag cgcgcaggtg
     2581 gtgctgaggt ttctggagca cctgtgagtc ggcggaggtt cgccccaagg gcctggttca
     2641 gaatgcaggt gattgacaga accgcccgcg agaccggccg gcgacatcgg gtaattcgca
     2701 acgcgtgcaa gatttcctgt gggtcaggac cgctgtggtc ctgacattcg tgccatcgac
     2761 gaggacggcc ccgtcaggac catgaaggtc ctgactacaa gaaccccgcc ccggctatga
     2821 cctcttgtag gtcaggaccc ttgtggtcct gacattcttg ccatcgaagc aagcggcccc
     2881 aatggggagc attacgcggc ctcgcctgcc ttgcgcgatg ggccgcggat cagcagcggc
     2941 ggtaatgcat gtagtaaatc ggccggccct cggcccgcca cttctgctcg aagaacgtcg
     3001 gcgcgtaact gaacagggcc tccgccgcga caaaggggcg gccgaggctg cgcagcccgg
     3061 cgacggcggc ggcgttctcg accgcccact gggcatacca gtcgacgtcg gtcgccatga
     3121 acaggtcgcc gtccggccgg agcacggccg ccagcagttc gacaaaggat ttgtgcagga
     3181 ggcgccggaa ttcgtggcgg gctttcggcc acgggtcggg gaacagcaca tagattttct
     3241 cgaaagccgg gcggccgtag aagcggggca acacgatgcg ggcgtcggaa cagatgagct
     3301 ggatgttggc caggctgcgc ttctgaatgc gcgggataag cttgtagtag cgcttcttgc
     3361 cgatttcgac ggccacgaac tgtttttccg gaagcgcctc ggccagcgac aacagcaggt
     3421 cgccccgccc ggggccgatc tcgaggatat ccccctctcc gaccgcactc ccctccggat
     3481 acaccaggat ctctttccgc tcctcccagg cccagggaaa ctctttcatc ggcggccgtt
     3541 gctcctgtcg tcgcgttggc ccggactgtt cctgcgcggg cgtgtccgag ggatcaggcg
     3601 ggagctgtcc gggtgggaca acataggacg cgcggcggag aaagtcaacc ggggcggtcg
     3661 agttcggccc ggagggtgtc gaccaggtcg gcgtgtttga atcggaatcc ctgggtgctc
     3721 aggcgcgccg ggaccgcccg gcagccggag aggatggtct cgcggcccat ctggccgaag
     3781 acggcgcgga tcacgccggc gggcacgcgg aacagggtgg gccgctgcag ggccgcgccg
     3841 agcgcctggg taaactcggc gttggtgacc ggctgaggcg ccacgacatt gaccggaccg
     3901 cggatcggtt cgttggtgat gaaatactcg aaagcgcggg cgacatcctc cagcgtcacc
     3961 cagggcaggt actggcggcc gtcaccgagc cgcccgccca gcccgagacg aaagagcggg
     4021 aggattttgt ggagcattcc cccctgtcgg ctgagcacga ccccgaggcg gaaattgacc
     4081 acccggatgc cggcctcggc ggccggctgc gcggcctctt cccactgccg gcagacgtcg
     4141 gcgagaaagg tatcgccggc ctgcgcggac tcatcaaccg cgcgctcgcc ggtgtcgccg
     4201 tagtagccgg tggccgaggc ggagagaaag gctttcggtt ggggatcgag tgcggcgcag
     4261 agcccggcca ggaggcgggt ggtctccacg cggctgtcgc ggatacggcg tttccgttcc
     4321 tccgaccagc gcccctcgcc gatattctcg ccggcgaggt tgatgatgac gtcgaacccg
     4381 gccagggcga ccggctcgac cgcgccggtc tccgggtgcc attcgatttc ggccgccgca
     4441 tcgcgcacgg gacggcgcac gaggcgatag accaggtgac cgcgcgactg cagatgcggc
     4501 acgaccgccg aaccgatgag gccggaggct ccggcgacag caactttcat gcgcctctcc
     4561 tgattcagtg tccttgaaag cagagaaagg actgacgggt gcgggcgcaa caagttttct
     4621 gcggcccggc cgcgccgacc aacgccgggg cgggcgcccc cggggcctaa cccttggtgc
     4681 ggcgccggaa caggagaggg taggccagca ttccgatcgc aaatccgagc gcgagcacca
     4741 gcggaatcag gagcacctgg gagatgctca tttgccagac gaggaagcgg aaggtgacga
     4801 cagcggtgtt ctggacgatc agaatgaggg cgagcaacgc gccggcgccg atggcgatcc
     4861 gtttggcggt gcgcgccggg ggatttccgg cgttcgggtg cgacattgat tcctccacac
     4921 tcaggctaag gcccgcggcg cgggggcggc gccccgtcgg cggcgcgccc ggggcgcacc
     4981 ggaacccgcg gtgcgggcga taagaaagcc cggcgatcgg ctgatcgccg ggctggaatt
     5041 gtcggtcaag ccgttgggcg cggccgacag actactcggg gcgttccccg ccctcctccg
     5101 gcgacgtgcc ggcatcgggc ggctcagggt tctcctgatt cgcctgcgag gccatccggc
     5161 gaagttcctc cggcatcgct tccgccatgc tggtggtctc ctcgcgcggg ggggccgggc
     5221 gcgggtagtc gtcgcggtcc gcatcgcggt cgtgggtgcg gtcgtgggag cgctcgtcgc
     5281 gcggctccgg ggcggcctcc gcctgccggc ggagctcctc ggggatcgcc tcggccatgg
     5341 acgggccggc gttggccacc gggtgggtcg ccaggaagtt gtcgagttcg gtccgctccc
     5401 gcttcttgta gtaggcggat accgacagca cgatcttgtg ctggttgcgg tcgaattcga
     5461 tcacctgcag ggggatttcg tccccctcct tgaagacgcc ggtgggatcg gtgaggtctt
     5521 tgcgtccgag ctgggtagcc ggcacgaacc cctcgaccgc cccgtcgagc tccacgacca
     5581 cgccgcggtc gagaacgcgc tggatggtgc cgaggacctc ggcgccgacg ccgtacttgc
     5641 gcgcgatctc gggccaggga tcatcctcga gctgcttgta gcccagcgaa atgcggcggt
     5701 tctcgtggtc gatcttgagg atcttcacat ccacccgatc ccccttcttc atgacctcgg
     5761 aggggtgttg gatacgcttg gtccacgaca tgtcggagat gtgcacgagg ccgtcgatcc
     5821 cctcttcgag ttcgacgaag gcgccgaaag cggtgaggtt gcgcaccttg ccggtgacaa
     5881 tgcggccggg cgggtacttc atctcgatcg tggtccacgg atccggctcc atctgcttga
     5941 tcccgagcga gatcttctcg ttctccttgt ccaccgacag caccatcgcc tcgacctcgt
     6001 cgttcacgtt catgatcttg gaggggtgct tgatgtgctg ggtccaggac atttcggaga
     6061 tgtggataag gccctcgatg cccttttcga gttcgacgaa tgcgccgtag tcggtgatcg
     6121 agacgacccg tcccttgacc ttgcggccga gcgggtattt ctgctcgatg ttctcccacg
     6181 ggtacggcgt catctgtttc aggccgagcg agatgcgcga cgtccgttca tcgaagtcga
     6241 gaattttcac gtcgatccga tcgccgagcg agaccagctc gctggggtgg cgaatccgtc
     6301 cccacgacat gtcggtgatg tgcaggaggc cgtcgacccc gccgaggtcg atgaacacgc
     6361 cgaaatcggt gatgttcttg acgattccct ggcggacctg gccgaccgcg atctcattga
     6421 gcaggtgcgc ccgcatcttc tcgcgctcct cttcgagcac gatgcggcgg gagacgacga
     6481 tattgcggcg ggccttgttg atcttgatga tcttgacatc catcgactgc ccgatgagag
     6541 cgtcgaaatc cggcacctgg cgcagcgcca cctgtgatcc cgggaggaag gcatcgacgc
     6601 ccatgatgtc gaccacgagg ccgcccttga tccggcgcgc gacgatcccg gaaatcttct
     6661 ccccgctgtc gtggacctcg cggatcttct cccagacccg catgaaatcc gccttctgct
     6721 tggagaggat cagctgtccg tgggcgtcct cgatctgctc caggtagacc tcgatttcat
     6781 cacccatgcg gacggtgacg ggctgctgga actcgcccat ggcgatgatg ccctcggact
     6841 tgaagccgac gtcgacgatg acatcgtcgc ggttgatccc gatcacgcgc ccggcgacaa
     6901 cctcgccctc tttgatgtct ttgatggtct ggtcgtacat gtcgaccatc tgctggtact
     6961 cgtcggggtc gtagacgacg ccggcgatgt cggtgatgcg gacggcctgg acagcggacg
     7021 acccgtcgtc aagaccctcc tcggtgcggc tgaggcgctg ctgcgccttt tccgccaccc
     7081 gtttgtggcg gctcgtgaca actcgctcgc gctgctgctg gtcctccgca gacaaaccct
     7141 gctgctcgag gtcgatgccc tcgggccggg cggcttttgt cttgacgcgt ttggtctttc
     7201 gggtggattg cgctttgcgc ttggacagtc tctgagttgc ggtttcggat tgtttggccg
     7261 ttgccatgtt gactgtacgc tacctcctaa tcaagggccg acagtttgga attttcccga
     7321 gggaagactg ccggatcggc tgcccgccct ctcagagcgg gaagccttgc tatataacac
     7381 ataggatcgg aagcgcaagc gaattctgcg gtcctttttg gcacgttccc gccggccctc
     7441 ggtccgccgc accgggctga ttccccgccg ggagtgcgtc gtagggcgcc ctcacccggt
     7501 caacaacgac tctgtcttgt cgggccgccg cgccgaccgc catcgctttc tgccgcccta
     7561 cagcccggaa gcggccttga tcttcgcaat cctatccatg acctcccgcg ccaactgcac
     7621 gtacccctct ttggatttcg ggaaggatcg tacccactcg accggcaggg gttcgccgaa
     7681 cgcgacgcgc agcttctcgc gtcccaggag gcattgctta agcttatttg tcccatgaag
     7741 ataacatgga acgatcgggc actcggcgtg cagcgcaatc aaccccagcc cgggcttggg
     7801 ctccaagaac gagtcgtccc tggaccgcgt cccctccgga aacaccgtca gcccatatcc
     7861 cttgcggatc gtcgccaccg cagtcttgag cgcgtcccga tcgaccgatc cgcgcttcac
     7921 cggcatggcg ttggttctcc ggatgagcga tccgagcacc ggcaccgcga agagctctgc
     7981 cttggcgaag aaggccatct cccgtccggt ggccgatccc gccagcggcg gatcgaagta
     8041 ggagcgatgg ttgctcgcga gaatgaagcc gccggaccgg gggacgtgct ccttgccgat
     8101 gatccgcccg cgaaagatga cgcggcctgc gatcttcgcg atgaagtagc cggtccagta
     8161 gagaatcttc atagggttga tccgtccgac ggttcgggac atggtacagc ggggggccgg
     8221 ggatggcaat gaaaaagggc ggcttcgcag ccgtgatcgg ttcgtactca gcgtcggcca
     8281 gcggggccat ggacgttaag atttgcctgc ttgtgccggc acccgcacgt tatctgttga
     8341 cagggcggtg gcgccttgcg gataatgcac ctgagtaact atgttcccgg cgtcgccgtt
     8401 cggtcgtcac cgacaccggg ctaccggtca caaagcctta tcggagggag tatgttcagg
     8461 ctgttgattt gcagtggagc ggtgctcgcc atcgccggag cagtcgcggc tgcggaaagc
     8521 ccgattgatg agggcagcct ttacctcgat gggagcgtgt tcttccagag acaaagcggc
     8581 gaactgtggg gggacggtct taccagcgcg ggggtcggca acggcagttt ctcgctgcaa
     8641 gagggttttg aaatcggacc gacggtcggc tattttacgt cgccgggctt attcgtcggc
     8701 ggtcagttcg cgatcctgta ctatgaacag ggtaagtcca acctcgcggc aattgccctc
     8761 gggccgaccg tcggctattt cttcagaacc ggccagggcc gaaccgaggt gaagggaagc
     8821 gcgtacccgt atatccgcgg cttcttgagc tggggaaacc tcgttagcgg cggtgacgtt
     8881 gcgatttggc agtttggagg caagggcgga cttctgtaca tgctgtccgg ggcggtcgcc
     8941 ctcgacattg ccgcacgctt tcagggagac agttggaggc cggacggagg actctcaata
     9001 aacgggacca ccgtctcggt cggcatcggg gtttccgcct tcatttactg acctccaacc
     9061 ggtctgcctg gtttatgcga tgtttatgcc gcgaggatgc agggcgcggt cgcccgacac
     9121 ttttgtccgt cgaccgcccc ggtgatcctc aaccccgtcc ccggttctgc cgattaatcc
     9181 gtacatgaac cacttcccga ttcccgccat aacccgcacg agcgcctcgg ccgaggagct
     9241 cgcggtctac cgggcgctca agccctacct ggcgcgctgt ctgcgcctca atcacgatgt
     9301 caacaacgcg ctggccggca tcatcggcta cacggatttc ctcctcgaag ggggcagcgt
     9361 actcaccgag gaccagcggc ggtttctatc gcagatcatg caggcggccg agaaggtgcg
     9421 gcgcctggtc gaggagttgt gcgacgagaa agtcgccgcc gcccagtcga tcgacctcgc
     9481 ggcgctcagc cgcgcgctcg agcagatgga aaacgactca gactagctgg ttggcctttc
     9541 gcagcagcca ctccagggag agggcgccga tcgccgccag cagcatccac accttgttcc
     9601 agagtgtgaa ttcgcgccgc gtgctgaccg ggatttcggc caggtcgagg tcatcgagga
     9661 tccggtcgaa ctgccggtag gagtagtatc ttcccccggt ggtccgtccg agagccgcca
     9721 gcagatcggg gtcgccgctc tggtcgaatt cttcgagcga gaaggcctcg atgaggaagc
     9781 tcccgctgtc ggccctgagc aactcgccgt ccttgacgat cgcggcggcg tatcggtacc
     9841 gtcccggcgc gaggttttcg aaaacggcgc ggaaccgtcc ctccccctcg tcgacgagat
     9901 cgcgctggta ctcctcgtcg tcggggccgc cgcgcacctc gaccatgccg gtcgctcccg
     9961 gaatcggccg gtagcccaga tcgaacgcga aaccgttgaa gaggaccggt tctccccgcg
    10021 tgaagacgtc cttctcgggc tggacccgga cgggatcgaa gtcgtccttg accgtgagcc
    10081 atccgaccac gccgtcgaca aacttgcggt agttcgaatc gtcttcgccg aacccgaggt
    10141 tgacgaaccc ccagggccag aagggcagga cggcggaggc gaagagtttg cccggtccga
    10201 ggcggcggaa accgagggcc gggggggcgt aggccggggt gcggcgggag aattcggcca
    10261 gcacgacgcc gtcgggtgcg atcgaatcgc accacacgag ggactcgaac ggcggcaggc
    10321 tcgcccaggc ctcgcggatc gccgcctggt tgtcggccag gcggatcgcg gggtgaaaga
    10381 gatggctctc gagcggctcg gcccgcccct cgacatactg cagcggctgc gcgatccgct
    10441 ggcagaaggg gagcagatcg ttcagccacg aggtcggccc gcgggcggcg aactgcggcc
    10501 ccatgaacag ccagacggcc ccgcccttct cgcgcaggta cgaccggatg atttcctggc
    10561 gggaagtgta ggccgcagga tcgggatcgt acaggatcac cagatcatag cggttgagtt
    10621 cggtctgcac ggtcgggaag cggccggcga ggttgccggc gcgcgccccc gtggccagca
    10681 gcgtcacctc gtaacggtcg gatccgtcga gcagccgctt gagaaagccg acctcgtagt
    10741 cgggccggtc ggccgcgagc agcacgctca agcggctctt gaggactttc accgaaatgg
    10801 tccgctggtt gttggccgcc gactcctcgt tctcccccgg cggaagggca atctgcagga
    10861 ggcgctggcc gggggcttcg ggcgtgtacc gcagcatcac ctctccccgc ccggccgcct
    10921 gggtgacggt caggcggctc tcggccgccg tgcgcgccga gtcgaccaag cgcacgtcga
    10981 gcgtcctccc ctcggcgttg cgccaggtca gtttcacccg gatctcggtc ggccggccga
    11041 caaacagcac cgggttgaac gcaacttcat cgatcccggc gtcgaaactc tccgcgccgc
    11101 cggagacgtc gacgctgatc acaggggacc ggagcgacgg cgccacgtcg agcggcttgc
    11161 gcccggcgtt cgaccggccg tccgacagca gcagccagta gtcgctgggt tcggcggtct
    11221 gggccttgtc gagttcgaag agggcatcgc cgagcgccgt gctcgtgctg tcgaccgacc
    11281 cgcgggcggc gccgaggctg ccgccgaaga agaagggagc gaggtcagcg acccggcgga
    11341 ggttgtcgag agtcgcgctc gccagcagcg aatcggtccg ggcgcggcgg ctgcgcccgt
    11401 cctccgtctt gttcatcgag agcgactggt caaccaggac ggccacgcgc ggccggcgca
    11461 cgtattcccg ggtgtagctg acgaccggct ccaggagggc caggaagacc aggaggagcg
    11521 cgccgatccg cagtccgccc aagatcgccc gcaggtatgc cggcaggggc ggattggtcc
    11581 gccggtagag gtggacggcc agcccgacaa gggcggccag cgcgagggcc acgatcactg
    11641 gggagttggt caggacgttt atggagaggt cgtggaagcc aaacatactg ctttttatac
    11701 cccggtcggg gggcgcgggt tgtcgcggcc ggcgcgcgcg gggcgggctt tcccggccgc
    11761 cctcaggagg cccgcgaaat ggtgaactgc accaggctca cgaggcaatc gaagtattcc
    11821 gagggcgtca ccggcgtgat cgccgccagc ccctcctccg acagcgccct ggcccgcccg
    11881 taggcatgct caatgccgcc ctgctcgcgc acgaaggcca gcaccctctc gaacgagggc
    11941 cgccggtcgc gccgccgcag gtgggcgatg atctcgtcgt ggccgccccg gcccgccttc
    12001 ttcagggcat ggatgagcgg cagcgtcacc ttccccgtga gcacgtcgtt ccccggctcc
    12061 ttccccgtga tttccgcatc gcccacgaaa tccaggaggt cgtcgacgat ctggaaggcg
    12121 gtgccgatct tctcgccgaa gtgggcgaag cgctggcggt cgcgcctgcc gcgcccggcc
    12181 aaaatcgggc cggtctcgca ggcgaggctg aacagcgagg cggtcttgtc ggcgatgatg
    12241 gtcaggtact cctcctccga cagcgcgtag ttgcccgttt cttcgatctg g
//
LOCUS       DAOPOE010000090        12241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6003_length_12241_cov_5.595273, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000090 DAOPOE010000000
VERSION     DAOPOE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6003_length_12241_cov_5.595273"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKM94_10970"
     CDS             <1..491
                     /locus_tag="PKM94_10970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD67195.1"
                     /translation="AELALPRLPGLRFVLFGDGPDLERTRHEIESRGLNDRVLCPGFE
                     RDLAACLRGADLVVNPSLSEGLPNVVLEAMALGVPVVATAVGGVPELIRDGDTGLLPP
                     PADPRQLADALVRMGDDSALRRRVAANALNLVRESFSFEKQARELFALYADVLNQRDR
                     TV"
     gene            488..1633
                     /locus_tag="PKM94_10975"
     CDS             488..1633
                     /locus_tag="PKM94_10975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67196.1"
                     /translation="MIHIAFVIDTIATPGAGTEKQLLLLVRHLDRSRFEPHLLCLQSS
                     PWLERNSLPCPVEIIGVRSLRSPATTGALLRFRRYVRRHRIDIVQTFFIDSNLFGTVS
                     GWLSGVPVIISSRRNFGKDYWHTPAWLFLLRLLRRLTTCYIANSRLTADYSIEVERIA
                     ADRMHVIYNGLDFAPFANLTPELRAAARSAWGVADRHVLIGIIANLRPIKNHRLFVEA
                     AARVHSRRPDTRFLIAGDGDQREPIECRLHELGLQDTAILTGQVADVLPVLAALDIGV
                     LCSHGESLSNAIIEYMAAGIPPVVSDVGGNREAVGGEHGLVFPPDDLDAFVACLDRLI
                     ADPALRTRLGRAAADYARCTYEYREAVGRHETLYETCRAAARPRRRS"
     gene            1892..3097
                     /locus_tag="PKM94_10980"
     CDS             1892..3097
                     /locus_tag="PKM94_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67197.1"
                     /translation="MKLFRTALIAVVIVGLLAGGAMTYKLYRGHAFIWLPDYLADSPG
                     FAATADLTDIIFVVVDHWEPGGKPEIVNRWMTDYRAVAEKHRDANGVPLRHSWYYPIE
                     AFRGWEVDSLVKLCRLGLGDVEVHLHHFDDSSASLREKLSAGLDSLRAHGALVTPDGR
                     SRFSFIHGNWALDNSGAPVGDRMPCGVNDEISILLDLGCYSDCTFPAMGQTAQPSLVN
                     KIYYAVDDPAKPKSHDTGVRSAVGLATTPDQLQIFEGPYMIDWSDWRFKTHPTFDDGN
                     LYWEIPSSLHRFEVWLKANVHVKGRPNWVFVRPFTHGADLRRGGDSNILGENFDRMLT
                     DIEKRYNDGRRYRLHYMTAREAYNVVKAAEAGLEGNPHDYRDYLLKPYLYPETAVGKD
                     SLAAAVPAG"
     gene            3281..4648
                     /locus_tag="PKM94_10985"
     CDS             3281..4648
                     /locus_tag="PKM94_10985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67198.1"
                     /translation="MNWTIRFRLALWDFLRFHPACRFAASAAIRLYLRLPALRRDESL
                     LRAARLNVRAARICPHPGLLRRLERSLDASVGRLLRTNFDWDALSHESRPDQVAKGII
                     LKHPVSPREKGVLFISFEGQWLRLFRHADIDRLARDYHLVIAPSWSPPHSLAFSVAAR
                     RWPGRFFTLISNLNEIPVFARIAPNSEVVPLFGSSWVHPQIFDVSAPVAKEYDIVILA
                     NFAAYKRHFLLFRALRDMRPDTRVLLLGKSMDGRTPETIMNEARAYGVADRITIKPGL
                     PDGEMIRAFRSARVALMLSGNEGSCVAVVESMFADIPIGVFEDAVIGSLAYVNEHTGR
                     RLRRRRLGRQLEEFIADSGRYSPRRWVMENGISCFGSTAVLNDALRARVLEWGEEWTT
                     DLAPHHWRPNPTYVYDEDLERHRSAYARFERDYRCSLLLRATDGSPLTSALSARPSSP
                     TALPR"
     gene            complement(4596..5813)
                     /locus_tag="PKM94_10990"
     CDS             complement(4596..5813)
                     /locus_tag="PKM94_10990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD67199.1"
                     /translation="MAEMTPQASKERGKSAPEAVRVLIVCMWPLGGIRTYLKYNYKYF
                     PQDLFSITLLANPAIEREAIAADMAALGIRVEWAPPLWGKNLLCRHVYLLKRRERFDV
                     IHSQGFISAFHTALVNWWGHVPHVMTTHGVLEEKRFAGRFGRWKRAVFQRALRDVDVF
                     VGVGRDILAHVREGLPALRERARWEVIRNGIDPEPFVRDYPGAAERLRERLGIDGETF
                     LFGYFGRFMPEKGFVHLIEAAERLAGAGAARRFAVLAVGSGDYEREYRAMTVQLGVHE
                     LFRFQPFQANVAELLQGCDAVVMPSVWEAYPLLTSEVLCSGTPLVATTCPGLREAVEG
                     TPAVTVAPGDPAALAEAMRAVMEDPGMRERFRASRAEAARRFDVRESAERLAALLTEA
                     GRSATTDGPRGRT"
     gene            complement(5837..6577)
                     /locus_tag="PKM94_10995"
     CDS             complement(5837..6577)
                     /locus_tag="PKM94_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD67200.1"
                     /translation="MSRVLKLVLAASLVGNLAIVWVGYKAYQYRTHINYWLDKYTEVV
                     EEFSGRSRYRQDNERLRSDTAVPGRVVFLGTQVISRWDVGRSFPRWEAIDRGVAGQRL
                     AGMLLRFQPDVLDLGPEAVVIEISSYNFRPEWPVRELEDYAESMADLARARGVAPIIG
                     SVIPPWDADLGLGDYAIRDSIAAFNEWLQQGMAEGRWQVADFHGALADGEGYLRRELA
                     AESIDPNRAGYERMTAAVDSVLGRALGR"
     gene            7073..7657
                     /locus_tag="PKM94_11000"
     CDS             7073..7657
                     /locus_tag="PKM94_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD67201.1"
                     /translation="MIRKICRIVAVTILGASLAPSSFGENITLTWTAPGDDGTVGTAA
                     AYELRYSTHGITALNWFQAMPVIGLPIPRSAGTVQSFTLANLDPAQTYYFAMRARDEA
                     GNWSPVSNNAVWALCDCRNRRGNADGDPEDRVTVSDLLHLIHYVFRGQAGLPCLEEAN
                     INGSPDGIIDVADVNLLIAHLYREDQVFLPAPCQ"
     gene            7877..8692
                     /locus_tag="PKM94_11005"
     CDS             7877..8692
                     /locus_tag="PKM94_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD67202.1"
                     /translation="MYQRYAPGTPDCLRPTRLRLVPTLVLGLLLTGGLTAEVRAQLKA
                     APAAAGVVLSWTAPGDDGNTGTAASYDLRYARVPITAANWNDWGVMTACLNEPAPLAA
                     GTVQSYTIDSLDPSTTYYFAIKAADEAGNWSALSNVVSYATPAEDVPPAVITDLRVAA
                     VGQTSVTLGWTAPGDDGTTGTAASYTVKFSTSPITNANWAAATTVPGVPAPHAAGTAE
                     SLTVTGLTPGVTYYFAIKTSDEVPNTSGLSNVVSGTPTGDQTAPAAITDLDAS"
     gene            complement(8757..10352)
                     /locus_tag="PKM94_11010"
     CDS             complement(8757..10352)
                     /locus_tag="PKM94_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOD67203.1"
                     /translation="MKLHHLLARTAAVHGARPAVIHGERRISYGRLKEHADRLAARLA
                     ALGYAPGERAALLAENSIEYVIAYFAAAQAGLAVVPLDTSLRPAQVRELIGDSGSRVL
                     LTQTRFARFLAEISRAPSPLELIIAERALRLDLAAPVESLHDLLGPIDDQPPDPADEA
                     GGGGPDEDRLRELWSGAAAAPKDELAALFYTSGSTGTPKGVMLSHRNLSANTAGTVEY
                     LRLSCRDRVQVILPFYYIYGNSLLLTHAAVGGCLVIDNRFTYPETVLDTMERERVTGF
                     SGVPSNFMILLGNTTFAKRAFPDLRYFTQAGGAMAPEVIRRLREAFPTKEIFIMYGQT
                     EASPRVSWLPPERLGEKLGSVGIPVPGVQVRLVDAAGNDVAAGEEGEIIVGGDSVMMG
                     YWKQPAEQAEVLREGWLYTGDLARRDDEGYLYIVGRRKEIIKSGGNRVSAKEVEETIL
                     ELTGIAEAAVYGVPDAVLGEAVAAAVVPGTGAGVSANDVIVHCKARLAGHKVPKHVQF
                     LAALPKYQSGKVNKQALKKTAPA"
     gene            complement(10383..11360)
                     /gene="nadE"
                     /locus_tag="PKM94_11015"
     CDS             complement(10383..11360)
                     /gene="nadE"
                     /locus_tag="PKM94_11015"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOD67204.1"
                     /translation="MEFHKDSLKIDAARVAESLRRVIAEQIGVQMKKRGAVVGISGGI
                     DSSVVAALCATALGPQRVVGVMMPEKDSSPESRRLAQLLAERFGFETILETLTGGLEG
                     MGCYRRRDEAIRQVFPDYGPGYKCKIGIPSTVIEQAAFNYFTLTVEDPQGRIETRRMP
                     MGVYLQVVAASNLKQRLRMTTLYYHAEKRNWAVVGTGNKDEHEQGFFVKWGDGGADLK
                     PIAHLFKTQVFQLARHLGVPDEIVSRTPTTDTYSAEQTQEEFFFGLDFYTMDMLWYAM
                     EHRVPAAEAARVLNLTPEEVERGYRAIQRKIDATAYLRMNPLEAPPEAR"
     gene            complement(11497..>12241)
                     /locus_tag="PKM94_11020"
     CDS             complement(11497..>12241)
                     /locus_tag="PKM94_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67205.1"
                     /translation="VCAPAEVRAEASAGTAEISFRASCPTAISGYNIYISTVPLGEAY
                     PGTALPGSVEAWNHPVFPGDTTPEDGVEVYRAEGLTDGVRYYVHVRTVFPDQSLSRPS
                     EEIAIVTGPRGTIELAARFASEQDGFSFAAGAYVRADDLANDLYFYPAEGRDFLASPD
                     RLGLLRATRLRVLPFRGELSEAAAGAATMSSLPTEPRVAVEEGDWVQVMTADKTFALV
                     KVLGLEGQGEGRKMRLFYVWSPAVGAPVF"
BASE COUNT         1908 a         4862 c         3376 g         2095 t
ORIGIN      
        1 ccgccgaact cgccctccct cgcctccccg gcctccgttt cgtcctcttc ggcgatggcc
       61 ccgacctcga gcgcacccgc cacgagatcg aatcccgcgg cctgaacgac cgcgttctct
      121 gcccgggttt cgaacgggac ctggccgcct gcctccgggg cgccgatctc gtcgtcaacc
      181 cttccctctc cgaggggctc cccaacgtcg tgctcgaggc catggctctc ggtgttcccg
      241 tggtggccac cgcggtcggc ggcgtgccgg aacttatccg cgatggcgac accggcctgc
      301 tgcccccccc ggccgatccg cggcaactcg ccgacgccct cgtgcgcatg ggggacgact
      361 ccgccctccg tcggcgtgtc gccgccaacg cgctcaatct cgtgcgggaa tcgttctcct
      421 tcgagaagca ggcccgcgag ctgttcgccc tctatgccga cgtgctgaac caaagggaca
      481 ggaccgtatg atccacatcg ccttcgtcat cgacaccatc gccacccccg gcgccggcac
      541 cgagaagcag cttctgctcc tcgtgcgcca cctcgaccgc tcccgcttcg aaccgcacct
      601 cctgtgcctc cagtcctccc cctggctgga gcgaaactcg ctgccctgcc cggttgaaat
      661 catcggcgtc cgctcgctcc gctcccccgc gacgacaggg gcgctgctgc ggttccgccg
      721 ctatgtccgc cggcaccgca tcgacatcgt ccagactttc ttcatcgatt ccaacctctt
      781 cggcaccgtc tccggctggc tttcgggggt tcccgtcatc atctccagcc gccgcaattt
      841 cggcaaggac tactggcaca ccccggcctg gcttttcctc ctccgcctgc ttcgccgcct
      901 gaccacctgt tacatcgcga actcccgcct gacagccgac tactcgatcg aagttgagcg
      961 gatcgccgcc gaccgcatgc acgtcattta caacggtctt gatttcgctc cgttcgcgaa
     1021 cctgacgccc gaattgcgcg ccgccgcgcg gtcggcctgg ggcgtggccg accgccacgt
     1081 cctcatcggc atcatcgcca acctccgccc catcaagaac caccgcctct tcgtcgaggc
     1141 cgccgcccgc gtccactccc gccgccccga cacgcggttc ctgatcgccg gcgacggcga
     1201 ccagcgcgaa cccatcgagt gccgcctcca cgagctcggc ctgcaggaca ccgccatcct
     1261 caccggccag gtcgccgacg tcctccccgt cctggcggct ctcgacatcg gcgtcctctg
     1321 ctcgcacggc gagagcctct ccaacgccat catcgagtac atggccgccg gcatcccccc
     1381 ggtggtgtcc gacgtcggcg gcaaccgtga ggccgtcggc ggcgaacatg gcctggtgtt
     1441 tccacccgat gacctcgacg ccttcgtcgc ctgcctcgac cgcctcatcg ccgaccccgc
     1501 cctccggacc cgcctcggcc gcgctgccgc cgattacgcc cgctgcactt atgaataccg
     1561 cgaggccgtc ggccgccacg agaccctcta cgaaacctgc cgcgccgcgg cccgcccccg
     1621 ccgccgttcc tgatcccccg acccttttgc gcgcctcgtc ccgagtcgtt gatttgcctt
     1681 gctctccatg gccccgccaa tatattgaga agtggctttt tgatgatcgc cgaagtcgtc
     1741 tgccggcggc cttcctgaat cggggaactt atcccccgcg cccgcggttt gcacagcagg
     1801 aacgcatcgg cggtctgcgt gtaagagacg tccccctcgg ccttaacagg acagatgtac
     1861 ccggccgctc actgaaggag atagacagcg aatgaagctc ttccgcaccg cgctcattgc
     1921 ggtcgtcatc gtcgggctcc tcgccggcgg cgccatgacc tacaaactct accgcggcca
     1981 cgccttcatc tggctccccg actacctggc cgacagcccc ggcttcgccg ccaccgccga
     2041 cctcaccgac atcatcttcg tcgtcgtcga ccactgggaa ccgggcggca aaccggaaat
     2101 cgtcaaccgc tggatgaccg actaccgcgc cgtcgccgag aagcaccgcg acgccaacgg
     2161 cgtcccgctc cggcactcct ggtactatcc catcgaggcc ttccgcggat gggaagtcga
     2221 cagtctcgtc aagctctgcc gcctcggtct tggcgacgtc gaggtgcacc tccaccactt
     2281 cgacgattcc tccgcctcgc tccgggagaa gctctccgcc ggcctcgatt ccctccgcgc
     2341 ccacggcgcg ctggtgacgc ccgacggccg cagccgtttc tccttcatcc acggcaactg
     2401 ggcgctggac aactccgggg cgccggtcgg cgatcgcatg ccctgcggcg tcaacgacga
     2461 gatctccatc ctcctcgacc tcggctgcta cagcgactgc accttccccg ccatggggca
     2521 aacggcccag ccctccctgg tgaacaagat ctactatgcc gtcgacgacc ccgcgaaacc
     2581 caagtctcat gacaccggcg tccgcagcgc cgtcggcctc gccaccaccc ccgaccagct
     2641 tcagattttc gagggcccgt acatgatcga ctggtccgat tggcgcttca agacccatcc
     2701 caccttcgac gacggcaacc tgtactggga gatcccgtct tcgctccacc gcttcgaggt
     2761 gtggctgaaa gctaatgtcc atgtcaaggg ccgccccaac tgggtgtttg tccgcccctt
     2821 cacccacggc gccgacctgc gccgcggcgg cgacagcaac atcctcggcg agaatttcga
     2881 ccgcatgctc accgacatcg aaaagcggta caacgacggc cgccgctacc gcctccacta
     2941 catgaccgcc cgcgaggcct acaatgtcgt caaagccgcc gaggccggac tcgaaggcaa
     3001 cccccacgac taccgcgact acctcctgaa accgtacctc taccctgaga ccgcggtcgg
     3061 gaaggactct ctcgccgccg ccgttccggc gggttgagat cggcgcggac gggctccttg
     3121 gcgggcggcg ccgagaggac accgggcgcc ggaatttggc cgagctccgt gggaatcagt
     3181 ttgccgccgg ccggcggcgc gaatatattg gccctgcgat tcccgaccag gcgcgtcggc
     3241 ctgtcccgca tagccgccgc agcaggagat gagcaagatc gtgaattgga ccataagatt
     3301 ccgcctggcc ctgtgggact tcctgcgttt ccacccggcc tgccgtttcg ctgccagcgc
     3361 cgcgatccgg ctctaccttc ggctcccggc cttgcgccgc gatgagtcgc tcctgcgcgc
     3421 cgcccgcctg aacgttcgcg ccgcccgcat ctgcccccat ccgggactcc tccgccggct
     3481 cgaacggagc ctcgatgcct ccgtcggccg cctcctccgc acgaactttg actgggacgc
     3541 cctctcgcac gagtcgcggc cggaccaggt ggccaaggga attatcctca agcaccccgt
     3601 ctccccgcgc gagaagggcg tgttgttcat ttcattcgag gggcagtggc tccgtctctt
     3661 ccgccatgcc gacatcgacc gcctcgcccg cgactaccac ctcgttatcg ccccgtcttg
     3721 gtccccgccc cacagcctcg cgttttcggt cgccgcccgc cggtggcccg gacggttctt
     3781 caccctcatc agcaatctca atgagatccc cgtctttgcg cgcatcgccc cgaattccga
     3841 ggtggtcccg cttttcggct ccagttgggt ccacccgcag attttcgacg tctccgcgcc
     3901 ggtcgccaaa gagtacgaca tcgtcattct cgccaacttc gccgcctaca agcgccactt
     3961 ccttttgttc cgcgcgctcc gcgacatgcg ccccgacacc cgggtgctgc tgctgggcaa
     4021 atccatggac ggccgcaccc ccgagacgat catgaacgag gcccgcgcct acggcgtcgc
     4081 cgaccgcatc acgatcaaac cggggctgcc cgacggggag atgatccgcg cttttcgctc
     4141 cgcccgggtc gccctgatgc tctccggcaa tgaaggctcc tgcgtcgcgg tcgtcgagtc
     4201 gatgttcgcc gacattccca tcggcgtctt cgaggacgcc gtgattggct ccctcgccta
     4261 cgtcaacgag cacaccggcc gccgcctccg ccgccgccgc ctcggccggc agctcgagga
     4321 attcatcgcc gactccggcc gctactcccc gcgccggtgg gtcatggaaa acggcatctc
     4381 ctgtttcggc agcaccgccg tgctcaacga cgccctccgc gcccgcgtcc tcgagtgggg
     4441 cgaagagtgg acgaccgacc tcgcccccca ccactggcgc cccaacccca cctatgtcta
     4501 tgacgaagac ctcgagcgcc accgctccgc atacgcccgc ttcgaacgcg actaccgttg
     4561 ctccctgctg ctgcgcgcca ccgacggttc tccgcttacg tccgccctct cggcccgtcc
     4621 gtcgtcgccg accgccctgc ctcggtgagc agagccgcca gccgctcggc cgactcccgc
     4681 acgtcaaacc gcctcgccgc ctccgcccga gacgcccgaa accgctcccg catccccgga
     4741 tcctccatca ccgcccgcat tgcctcggcc agcgccgcgg gatctcccgg cgccaccgtc
     4801 accgccggcg tcccctccac cgcctcgcgc agacccgggc aggtcgtcgc caccagcggc
     4861 gtcccgctgc acagcacttc cgaggtgagc agcgggtatg cttcccatac cgacggcatc
     4921 accaccgcat cgcacccctg cagcagttcc gccacgttcg cctggaaggg ctggaagcgg
     4981 aagagctcgt gcacgcccaa ttggaccgtc atggcccggt actcgcgctc gtagtcgccg
     5041 ctgcccaccg ccagcaccgc gaacctcctg gccgcgcccg ccccggccag ccgctctgcc
     5101 gcttcgatca ggtgtacgaa ccctttctcc ggcatgaatc gcccgaagta cccgaacagg
     5161 aacgtctccc cgtcgattcc cagccgctcg cgcagccgct cggccgcccc cggatagtcg
     5221 cgcacaaagg gctccgggtc gatcccgttg cggatcactt cccagcgcgc ccgctcccgc
     5281 agcgccggca acccctctcg cacatgcgcc agaatgtccc gtcccactcc cacgaacacg
     5341 tcgacatccc gcagcgcccg ctggaacacc gcccgcttcc accgcccgaa ccgccccgcg
     5401 aaccgtttct cttcgagcac gccgtgcgtc gtcatcacgt gcggtacgtg cccccaccag
     5461 ttcaccagcg cggtgtggaa cgccgagatg aacccctggc tgtggatcac gtcgaaccgt
     5521 tcgcgccgct tcaggagata cacatggcgg cacagaaggt tcttccccca gagcggcggc
     5581 gcccactcca ctcgaatccc aagcgccgcc atatcggcgg cgatcgcctc tcgctcaatc
     5641 gccggattcg ccaaaagcgt tatactgaaa agatcttgcg gaaaatactt atagttatac
     5701 tttaagtacg tccggatccc gccgagcggc cacatgcaca caatcagcac ccggaccgct
     5761 tccggcgcac tcttgccccg ctccttcgac gcttgtggtg tcatctccgc catcccggcc
     5821 tctccggtac gtttctctat cggcccagcg cgcgtcccag caccgaatcc accgccgctg
     5881 tcatccgctc gtatccggcg cggttcgggt caatggattc ggcggccagt tcccggcgca
     5941 aatacccttc cccgtcggcc agcgctccgt gaaaatcggc gacctgccag cgcccctctg
     6001 ccatcccctg ctgtagccac tcgttgaacg cggcgatgct gtcccggatc gcgtagtcgc
     6061 ccagaccgag atcggcgtcc cacggcggga tcacgctgcc gataatcggc gccactcccc
     6121 gggcccgcgc gaggtcggcc atcgactcgg cgtaatcctc gagttcgcgt accggccact
     6181 cggggcggaa attgtacgag ctgatctcga tcaccaccgc ctccggcccc aggtcgagca
     6241 catccggctg aaagcgcagg agcatgcccg ccagccgctg cccggcgacc ccccgatcga
     6301 tcgcctccca gcgcggaaac gaccggccca cgtcccaccg cgaaatcacc tgggtcccca
     6361 gaaacaccac ccgcccgggg accgcggtat ccgaccgcag ccgctcgttg tcctggcggt
     6421 agcggctccg cccggagaac tcctccacca cctcggtgta tttgtccagc cagtagttga
     6481 tgtgggtgcg gtactggtac gccttgtacc caacccatac gatcgccaga tttcccacca
     6541 gcgacgccgc cagcaccagt ttcaacactc gactcatcgc cgcctcaccg tcatcgggaa
     6601 ctcctcctcc ccgccccgcc cgcgccccgc ggggggtccg cgaaaccggt gaacgatacg
     6661 ggcaaatgcg gcgcaagtcg agtgaaaaac atctcccgcc tcttcccatg gcccgcctcg
     6721 cccggcggcg cacgccgctg cacacgctgc agagaaatgc gccgcgcaga tcttgcttcg
     6781 cctgcagaaa atatttggtt caattccgcc gcatcgctat tatctgtgcg accgatcaga
     6841 ggggtaaact gattagtcgc ctgcttcggc agggggataa aaagcgtgaa tgggaagtta
     6901 atcgcggccg ggttccgccc ggaaccgtcg cggccgcact gtccacagta ctgcccggta
     6961 agtcctacct ttgcaagcgg acctggcgta tttcattccg tactcagcgg tgctaaccgc
     7021 tctcattacc tgccccccat gttccaaccg ttctgttacg gaggttcgct tcatgatcag
     7081 gaagatctgc cggatcgtcg ctgtgacgat cctcggagcg tcgctcgccc cgagctcttt
     7141 cggcgagaac atcacgctca cctggaccgc gcccggcgat gacggcaccg tcggaaccgc
     7201 cgcggcttac gaactgcgtt attcgaccca cggcatcacg gccctgaact ggtttcaggc
     7261 catgcccgtc attggcctgc ccatcccccg gtccgccggc acggtgcagt ccttcaccct
     7321 cgccaacctc gaccccgcgc agacctacta cttcgccatg cgcgcccgcg acgaggccgg
     7381 caattggtcg cctgtctcca acaacgccgt ctgggccctc tgcgactgcc gcaaccggcg
     7441 cggcaatgcc gacggcgacc cggaggaccg cgtcaccgtc agcgacctgc tccacctcat
     7501 ccactacgtc tttcgcggcc aggccggcct cccttgcctc gaggaggcta acatcaacgg
     7561 cagccccgac ggcatcatcg acgtggccga tgtcaatctg ctgatcgcgc acctctaccg
     7621 cgaggaccag gtgttcctgc cggcgccgtg ccagtgacgg gcgctccctc gcggcgccgg
     7681 cctttcttcc gccggcgccc ccggaggagt ctgtacgcga tctgacccct tgtgcttatt
     7741 ttcaccagcc gccgccgccc ggagaccccg actcggccgc tgcggccgcc taactgcttg
     7801 tcccctaacg cccgatttct ccccctcgcc ggcactcgat ttgcccctct ccccaacgtc
     7861 agaatacgga ggtgttatgt accaacgcta cgccccgggg acaccggatt gtctccgccc
     7921 tacccgcctg cgccttgtcc ctaccctggt ccttggtctt ctgttgaccg gcggcctgac
     7981 cgccgaggtc cgtgcccagc tcaaagccgc gccggcggcc gccggcgtcg tcctttcgtg
     8041 gacagcaccg ggcgacgatg gcaacacggg gacagccgcc tcgtacgacc tgcgctacgc
     8101 ccgcgtcccc atcaccgccg ccaactggaa cgactggggc gtcatgaccg cctgcctgaa
     8161 cgagcccgcg ccgctggccg ccggcaccgt ccagtcgtat accatcgatt ccctcgatcc
     8221 gtcgaccact tactacttcg ccatcaaagc ggccgacgag gccggcaact ggtcggcctt
     8281 gtccaacgtc gtctcctatg cgaccccggc tgaggacgtg ccccccgcgg tcatcaccga
     8341 cctgcgcgtc gccgccgtcg gccaaacgtc cgtcaccctc ggatggaccg cgcccggcga
     8401 tgacgggaca accggcaccg ccgcgagcta caccgtgaaa ttctcgacct ccccgatcac
     8461 caacgccaac tgggcggcgg cgaccaccgt ccccggcgtc cccgcccccc acgccgccgg
     8521 taccgccgag agcctcacgg tcaccggcct gaccccgggc gtgacctact atttcgcgat
     8581 caagacgtcg gacgaagtcc ccaacacatc cggtctgtcc aacgtcgtct ccgggacgcc
     8641 gaccggggat cagaccgccc ccgcggccat caccgacttg gacgccagtt gatgcgtgtg
     8701 aaacctgccg gcccgcggcc gacagcctcc ttttgtgtgg aagagaaacg gccccgctac
     8761 gccggggccg ttttcttgag ggcctgcttg ttgaccttcc ccgactgata cttcggcagg
     8821 gcggcgagaa actgcacgtg tttcggcacc ttgtggccgg ccagccgcgc cttgcagtgc
     8881 acgatcacgt cgttggcgct cacgccggcg ccggtccccg gcaccaccgc ggccgccacc
     8941 gcctcgccca gcacggcgtc cggcaccccg tacacggcgg cctcggcgat cccggtcagt
     9001 tccagaatcg tctcctcgac ttctttggcg ctcacccggt tcccgccgct cttgatgatc
     9061 tccttgcgcc gccccacgat gtacaggtat ccctcgtcat cccgccgcgc aagatccccc
     9121 gtgtacagcc acccctcgcg cagcacctct gcctgctcgg ccggctgctt ccagtaaccc
     9181 atcatcaccg agtcccctcc gacaatgatc tccccttcct cgcccgcggc gacgtcgttc
     9241 cccgcggcgt ccactagcct cacctgcact cccggcaccg gtatgccgac cgatcccagc
     9301 ttctccccca gccgctccgg cggcagccac gacacccgcg gggaggcctc cgtctgcccg
     9361 tacataatga aaatctcttt ggtcggaaac gcctcgcgca gccgccggat cacttccggc
     9421 gccatggccc cgcccgcctg cgtgaaatac cgaaggtcgg ggaacgcccg cttggcgaag
     9481 gtcgtgttcc ccaggagaat catgaaattc gagggcactc ccgagaaccc cgtcacccgc
     9541 tcccgctcca tcgtgtccag caccgtctcc gggtacgtga accggttgtc gatgaccagg
     9601 cagcccccca ccgccgcgtg cgtgagcagg agcgaattgc cgtagatgta gtagaacggg
     9661 aggatcacct gcacccggtc gcggcagctg agccgaagat actccaccgt cccggccgtg
     9721 ttcgccgaga ggttccgatg cgacagcatc acccccttgg gcgtcccggt gctccccgag
     9781 gtgtagaaaa gcgcggccag ttcatctttc ggcgccgccg ccgccccgct ccataattct
     9841 ctcagtcggt cctcatccgg acctccgccg ccggcttcgt ccgcgggatc cggcggctgg
     9901 tcgtcgatcg gcccgaggag gtcgtggagc gactccaccg gtgcggcgag atcgagccgc
     9961 agggcgcgtt cggcgatgat cagttccagc ggcgacggcg cccggctgat ctccgcgaga
    10021 aaccgcgcga accgcgtctg cgtgagcagc acccgcgacc ccgagtcgcc gatcagctcg
    10081 cgcacctgcg cgggacggag cgaggtgtcc agcggcacca ccgccagccc cgcctgcgcc
    10141 gccgcgaaat aggcgatcac gtactcgatc gagttctccg caagcagagc cgcccgctcc
    10201 cccggcgcat acccgagggc cgccagccgc gcggccagcc ggtccgcgtg ctccttcaac
    10261 cgcccgtagc tgatccgccg ctcgccgtgg atcaccgccg ggcgcgcccc gtggaccgcc
    10321 gccgtcctcg ccagcagatg gtgaagcttc accgcagttg cctttcctcg cgggccgacg
    10381 ccctaccgcg cctcgggcgg cgcctctagc ggattcatcc gaagatacgc ggtcgcgtcg
    10441 atcttccgct gaattgcccg gtatccccgc tccacctcct ccggcgtcag gttgagcact
    10501 ctggccgcct ccgccgccgg cacccggtgt tccatcgcgt accagagcat gtccatggtg
    10561 tagaaatcga gcccgaagaa aaactcctcc tgcgtctgct ccgcgctgta ggtgtcggtc
    10621 gtcggcgtcc ggctgacaat ctcgtccggt acgccgaggt ggcgggccag ttggaacacc
    10681 tgcgtcttga acaggtgggc gatcggcttg aggtccgccc cgccatcccc ccacttcacg
    10741 aaaaagccct gctcgtgctc gtccttgttc cccgtcccga ccaccgccca gttgcgcttc
    10801 tccgcgtggt agtacagcgt cgtcatgcga agccgctgtt tcagattgga ggccgccacc
    10861 acctgcaggt acacgcccat gggcatccgc cgcgtctcga ttctcccctg cgggtcctcc
    10921 accgtcagcg taaaatagtt gaacgccgcc tgctcgatca ccgtgctcgg aatcccgatc
    10981 ttgcacttgt accccggtcc gtaatccggg aacacctgcc ggatcgcctc atcccggcgc
    11041 cggtagcagc ccatgccctc caggcctccg gtgagcgtct cgaggatcgt ctcgaatccg
    11101 aaccgctccg ccaggagctg cgccagccgg cggctctcgg gggaggagtc cttctccggc
    11161 atcatcaccc cgaccacccg ctgcggcccg agcgctgtcg cgcacagcgc cgccacgacc
    11221 gaggaatcga ttccgccgga gatacccacc accgccccgc gcttcttcat ctgcaccccg
    11281 atctgctcgg cgatcacccg ccgcagcgac tccgccactc gcgcagcatc aatcttcaga
    11341 ctgtctttgt gaaattccaa ggcaaaaccc tcgtctatcg catctccaat tttccgttcc
    11401 ggcttttccg ctcgccgcag cccgctcttt gcccgcccgc cggactcccc atctttctgc
    11461 tcggtcctcg cgcgagcccg gcctccggcc ccggtctcag aacaccggcg cccccaccgc
    11521 cggcgaccag acatagaaca accgcatctt tcgcccctct ccttgcccct ccagtcccag
    11581 cactttcacc agcgcgaacg tcttgtccgc cgtcatgacc tgcacccaat ccccttcctc
    11641 gaccgccacc cggggctcgg tgggcagcga tgacattgtc gccgcgccgg ccgccgcctc
    11701 gctcagctcc ccccggaacg gcagcacccg caggcgggtg gcccggagca accccagccg
    11761 gtccggcgag gcgagaaagt cccgtccttc cgccggataa aagtacaggt cgttcgccag
    11821 gtcgtccgcc cggacatatg ccccggccgc gaacgaaaag ccgtcctgtt ccgaggcgaa
    11881 gcgggccgcc agttcaattg tcccgcgcgg gccggtgacg atcgcaatct cctccgaggg
    11941 ccgcgagagc gactggtcgg gaaagacggt ccgcacatgt acgtagtatc ggaccccgtc
    12001 cgtcagccct tcagcccggt acacctccac gccgtcctcc ggcgtcgtgt cgccggggaa
    12061 cacgggatgg ttccaggcct cgaccgatcc cggcagcgct gtccccggat aggcctcgcc
    12121 cagagggacc gtgctgatgt agatgttgta cccggagatg gcggtcgggc aggatgcccg
    12181 gaaactgatc tcggccgtcc cggcgctcgc ctcggcgcgc acctccgccg gagcgcagac
    12241 g
//
LOCUS       DAOPOE010000091        11902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6204_length_11902_cov_5.461805, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000091 DAOPOE010000000
VERSION     DAOPOE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_11902_cov_5.461805"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKM94_11025"
     CDS             <1..395
                     /locus_tag="PKM94_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD67206.1"
                     /translation="AGASGYVLKDGAFTELNEAIRIVTRGGRYLSPSIVDIVVEDYAQ
                     RLSPALGSALEKLSVREREVLQMIAEGHSTAAIAERLHVSRKTVETHRKNMMAKLELR
                     NVAELTKFAIREGLTSLDISTRKGDETE"
     gene            complement(456..1913)
                     /locus_tag="PKM94_11030"
     CDS             complement(456..1913)
                     /locus_tag="PKM94_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD67207.1"
                     /translation="MLALKHLNERLLRAEYAVRGPIVQRAQELEAAGRRIIYCNIGNP
                     QALKQRPLTYLRQILCLIEYPELLENADVTRHFPKDVVERARSILVRHPHGTGAYSQS
                     AGIPFIRKAVADFVARRDGIPASPEHVILTDGASKGAQAVLTALLRRRNDGFMIPIPQ
                     YPLYSASLELFGGRQIGYLLDDDDHWQLNEAVLEDSLAAARADGINPVGIVVINPGNP
                     TGAVLTVDNIKMIIAFAERHRLAIIADEVYQENVYAPGCRFHSFAKVMHTLGVVSVPL
                     FSLHSVSKGFLGECGHRGGYLEIRNVSEEVLAQFIKLQSISLCANIPGQIATYLMVSP
                     PQPGERSYDAYVQERDAILDDLKAKAEILGEGINQIPGMSVDIPQGAMYAFVRFRLPE
                     EKGADLTRLTPEQLSAYVAKRDTDYCLALLEQTGICVVPGSGFGQKPGTFHFRMTFLP
                     PRDEIEALVEKLGAFHRSYAASHVAEDGVAAMSRC"
     gene            complement(1913..3349)
                     /locus_tag="PKM94_11035"
     CDS             complement(1913..3349)
                     /locus_tag="PKM94_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOD67208.1"
                     /translation="MDMNSLTALLLDLSIIALLIVGITLFRTPRGARFGNLTAAAALL
                     AAIVLVLSRNAIFDPAWLVVALLIGSAVGALVAMRVTMIQIPAMVAFQHGAGGVAACT
                     IALVELTRTGVVLTPVGELSGYLGLVIGAATFSGSMLASAKLAAKLRQTPTLLPRHNL
                     LLLALLAVIAGLCAAAAGAVGSSLMALLLGLLVASVGLGLLFAIRIGGADMPVLISFL
                     NATAGLAAAFCGVVIQNRLLIAAGATVAASGSILTAVMCKAMNRSFVKVLSGAKQKSA
                     GSAVAEAPARQPVTRRTQTYTEAMAEAIQVLNRADRIIIIPGYGMALAKAQFQVATLA
                     RTLIELGKRVSFAIHPVAGRMPGHMNVLLAEADVDYELLREMDEVNPEFAETDVVLII
                     GACDVVNPAAISRADTPISGMPILNAHEARHVLVLNLDERPGYSGVPNPLYDEPTTLL
                     LFGDANKSVTDLLLSVQAEPARQQGVAV"
     gene            complement(3502..3810)
                     /locus_tag="PKM94_11040"
     CDS             complement(3502..3810)
                     /locus_tag="PKM94_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOD67209.1"
                     /translation="MTDLILMLLVFALSFGAGYLLISRVPPLLHTPLMSMTNAVSAVT
                     ILGAILLFAVPSGAGERVIGALALITAAFNLVGGFVITDRMTRLFKTQHDPANRTDGA
                     "
     gene            complement(3834..5003)
                     /locus_tag="PKM94_11045"
     CDS             complement(3834..5003)
                     /locus_tag="PKM94_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOD67210.1"
                     /translation="MRIVIPKEILHAEKRVAAIPETVAKFRQKGFEVAVEAGAGEGIF
                     VSDSDYEKAGAAIVRDVEELFGQADLILKVKQPIFNEAREKHEVDMMPSRALLVTFLH
                     PASPDSHDIVRKLAARRITSLTMDGIPRISRAQRMDALTSMSTVAGYRAVLDAAEYLP
                     RFLPMVGTAIGAIKPATVLVVGAGVAGLQSIATAKRLGAVVKAWDIRESARQEAASLG
                     AKVEGFDVPADLAIAPGGYARQLPEEWLRKEQAAIGAIAAQADIIILSALVPGSLAPT
                     IITEEIVARMRPGSVIIDISIDQGGNCALTKPGQDVWAHSVRICGVQNLPGRMPMQSS
                     WLYANNMYYYIENLFKNGPGTPDFEDEIVRASLVTTDGRIVYEPALEAMGERVTV"
     gene            complement(5037..5846)
                     /locus_tag="PKM94_11050"
     CDS             complement(5037..5846)
                     /locus_tag="PKM94_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67211.1"
                     /translation="MNRRYVETKVLAQHGPSTHLLIKAGDTVVQANYAEVAPCAFPVW
                     TGAPVYVEIPAHGVKNSVPGSFSRAPEPQDASGELPNIIKFPVYTHQEETALQEFEDL
                     AGPSGRSNGATGPGSRLPVVAIAAAAGALMNDDRRIETHRVLPVWNDGTAVLVSLDWC
                     EGEPAEGTPNPEWHLRASNGLPLDLGLRHLAESAQLPAQAADSRYCLTVSPWKLGAAV
                     LGSPGHETAHKIWQREWRYDDLEEFRSALGRLRDALLGLTADLPSMAEQRA"
     gene            6460..7137
                     /locus_tag="PKM94_11055"
     CDS             6460..7137
                     /locus_tag="PKM94_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOD67212.1"
                     /translation="MKQLILMAGALLMLGIAASAQESPAGVTGKGVKLGLDFSRINTE
                     YDELDDFLDSRVGFTAGAFLTYRVSRQFAVQPEILYVAKGAEKDFFFYTPEWATEYLE
                     IPVLLKFDLMPAGPAHPNLFAGPALDFLLGSEFRVSDYRIDLSDYTQSVDVGLVFGGG
                     IDYGYFTFDARYTVGLIGMVDGFDDFAAAVNEATGAEPGDSYYLDGDLSVKITNTNLS
                     FMVGVRF"
     gene            7386..8456
                     /locus_tag="PKM94_11060"
     CDS             7386..8456
                     /locus_tag="PKM94_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOD67213.1"
                     /translation="MRFGAALLLAAAALFGPGPAAADDAGVPLTAVSAHSLGPERLGN
                     LPPVIVPVDNPQTDTKVLLGKQLYFDTRLSKDNTISCATCHNPAMGWSDAGPTSAGIN
                     SQRGGRRAPPVSNAAYSPLQFWDGRAPSLEEQAKGPIANPIEMGNTHDAMLRTVDGFP
                     EYVEEFKRVFGEVPITLDLVAQAIAAYERTIVTTDSPFDRFVRGDQSALTRQEKQGLE
                     IFNGKGHCTLCHWGGYFSDGRFHNLGVAPAPGAAYDEGRQAVTKDPADAAKFKTPTVR
                     DAAKRPPYMHNGTEKTLHDVVTLYNRGGGTDDANLDPLMLPLGLSRAEIDALVAFMER
                     AMVSSNPSVANEQPVAPEKMPK"
     gene            8468..9736
                     /locus_tag="PKM94_11065"
     CDS             8468..9736
                     /locus_tag="PKM94_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67214.1"
                     /translation="MSRMMRRGALAIGILLMVPQVSQGVPAFARKYGFNCNMCHTGFT
                     KLNDFGQLYRNSGYQIPGQEGLERTVFETAPPLAVRTSVGLFASHRPNANTAGFAIHG
                     LDVFSAGVLHKNISYLIVYTPRIDEPAADYTGPADGSAPSQLGGLESANLVFSNLVPD
                     AVNVRVGRFEPAYHPFSSKRSYYLLQPYEIYAFTTPSNGFVFDDNQLGVEVTGHFRRG
                     LFYALGVVNGSGANPDNSRAKDVYVRASQVFGRGEGQNAGQRIGLFGYFGWCPTITGD
                     PSPDAAGQGNGRAHAQFHRIGADLSLNWQTFNLHGMFLQGSDDQAINLPAVPADTADY
                     DFTGGFVQLDYAGLMNNRLVGSAAYNWVTPPDFDEECTISAFSLLVRYYLGDWTAVNV
                     ALHAEYTHREEGKADKIKTDQLGLLVDVGL"
     gene            10110..11072
                     /locus_tag="PKM94_11070"
     CDS             10110..11072
                     /locus_tag="PKM94_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOD67215.1"
                     /translation="MKRYSGNPLISRTDIPCIKPSLVDVSSVFNPGAVRWRGQVVLIL
                     RVQNRGRETFLLTAFSDNGIQFRIDRRPITFSGLERFPHRLHHIYDPRLTRIGDVCYV
                     TLAMDTDAGCRVGLARTTDFRAFEFLGEILHDEARNAVIFPERIGGAYAMLYRPNRTR
                     LEGGVASGDAIEWAVSENLQDWSPRGPVMAGRPHYWDELIGSGPPPLRTRHGWLHVYH
                     GVATHFAASNIYQAGVVLLAADHPGRVIARSRANILEPRELYELTGQVPNVVFPTGLI
                     PARDGGDGAVEDDAELLLYYGAADTCVGLATATVGELLEACDEV"
     gene            11062..>11902
                     /locus_tag="PKM94_11075"
     CDS             11062..>11902
                     /locus_tag="PKM94_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOD67216.1"
                     /translation="MKCDRCEGVPAARIALFVLLLAVVSGCRPEPGRGASAGSSGDTL
                     PVPAIPYAPRGYVCCRTAEGLTVDGRLEEAAWRQARWTELFTDIEGDRKPAPRFRTRA
                     KMLWDSTCFYIGAELAEPHVWATLTRRDAVIYHDNDFEVFIDPDADTHEYYELEVNAL
                     NTVWDLLLLKPYRDGGPAVNAWDIAGLQTAVAVEGTLNDPSDVDSGWRVEIAIPWAVL
                     AECAHRPSPPREGDRWKVNFSRVEWQVEARDGVYAKVTDPATGRPLPEDNWVWSPQGL
                     IAMH"
BASE COUNT         2003 a         3749 c         4172 g         1978 t
ORIGIN      
        1 cggcgggcgc ctccggctac gtcctcaaag acggcgcctt caccgaattg aacgaggcca
       61 tccggatcgt cacgcggggc ggacggtatc tcagccccag catcgtcgac atcgttgtcg
      121 aggactacgc ccagcggctc tcccccgcgc tgggttcggc gctggagaaa ctctcggtgc
      181 gggaacggga ggtgctgcag atgatcgccg aggggcactc gacggccgcg atcgccgaac
      241 ggctgcacgt gagccgcaaa accgtggaga cccaccggaa gaacatgatg gccaagctgg
      301 agctgcggaa cgtggccgag ctgaccaagt tcgcgatccg ggaggggctc acgtcgctgg
      361 acatttccac ccggaaaggg gacgagaccg agtgagcggt ccgggggcgg ccgcggcagc
      421 cctgggccgc cgcagccgga cacccgtgca tgcactcagc agcgggacat ggcggctacg
      481 ccgtcctctg ccacgtgcga agccgcgtag gagcggtgga aggcgccgag tttctccacc
      541 agcgcctcga tctcgtcgcg cggcgggagg aaggtcatgc ggaaatggaa ggttcccggt
      601 ttctggccga atcccgatcc cggcacgacg caaatccccg tttgctccag cagcgccagg
      661 cagtagtcgg tgtcgcgctt agcgacgtag gccgacaact gctcgggcgt caggcgcgtc
      721 aaatccgcgc ccttctcctc gggcaggcgg aaccggacaa aagcgtacat ggcgccctgg
      781 gggatgtcga cggacatgcc ggggatttga ttgattccct cccccaggat ttccgccttc
      841 gccttgaggt cgtcgaggat ggcgtcccgt tcctgcacgt aggcgtcgta gctccgttcg
      901 cccggctgcg gcggcgacac catgaggtag gtggcgattt gcccggggat gttggcgcag
      961 agactgatcg actggagctt gatgaactgc gcgagcactt cctcggagac gttgcggatc
     1021 tcgagatagc cgccgcggtg cccgcactcc ccgagaaacc ccttcgagac cgagtgcagg
     1081 ctgaacaggg ggacagagac gacccccagc gtgtgcatca ccttggcgaa cgagtggaag
     1141 cggcagccgg gggcgtagac gttctcctgg tagacctcat cggcgatgat ggcgaggcgg
     1201 tggcgctccg cgaaggcgat gatcatcttg atgttgtcga ccgtgaggac ggcgccggtc
     1261 gggttgccgg ggttgatgac gacaatgccg accgggttga tcccgtccgc gcgggcggcc
     1321 gcgagactgt cttcgagcac cgcctcgttg agctgccagt ggtcgtcgtc atccagcagg
     1381 tacccgatct gtctgccgcc gaagagctcg aggctggcgc tgtagagcgg gtactgcggg
     1441 atggggatca tgaagccgtc gttgcggcgg cggaggaggg cggtgaggac agcctgggcc
     1501 cccttgctcg cgccgtcggt gagaatcacg tgctccgggc tggcggggat gccgtcccgg
     1561 cgggcgacaa agtcggcgac cgccttgcgg atgaagggaa tgccggcgct ctggctgtag
     1621 gcgccggtgc cgtggggatg ccggacgaga atggaccggg cccgctccac cacgtccttg
     1681 gggaagtggc gggtgacatc ggcattctcg agcagctcgg ggtactcgat caggcagagg
     1741 atctggcgca ggtaagtgag ggggcgctgt ttgagcgcct gggggttgcc gatgttgcag
     1801 tagataatcc tgcgcccggc ggcctcgagt tcctgagccc gctgcacgat cggtccgcgc
     1861 acggcgtact cggcgcgcag caatctctcg ttgaggtgtt tgagggcgag catcacactg
     1921 ccactccttg ctgccgggcg ggttccgcct gcacgctgag cagaagatcg gtgaccgact
     1981 tgttggcgtc gccgaacagc agcagggtcg tcggctcgtc gtacagcggg ttgggcacgc
     2041 ccgagtatcc gggccgctcg tcgagattga gcaccaggac gtggcgcgcc tcgtgggcgt
     2101 tgagaatggg catgcccgag atcggggtat cggctcggct gatggcagcg gggttgacaa
     2161 cgtcgcaggc gccgatgatc agcaccacgt cggtctcggc gaactccggg ttgacctcgt
     2221 ccatctcgcg cagcagctcg tagtcgacgt cggcctcggc cagcagcacg ttcatgtggc
     2281 cgggcatgcg gccggcgacg gggtggatgg cgaagctgac gcgtttgccg agctcgatga
     2341 gcgtgcgggc gagggtggcc acctggaact gggccttggc caaggccatc ccgtagcccg
     2401 gaatgatgat gatgcggtca gcccggttga ggacctggat ggcctcggcc atcgcttccg
     2461 tatacgtctg cgtccgccgc gtcacgggct gccgtgcggg cgcctccgcg acggcgctcc
     2521 ccgccgattt ctgcttcgcc cccgagagca ccttcacgaa gctccggttc atcgccttgc
     2581 acatgaccgc ggtcagaatg gagccggacg cggcgaccgt ggcgccagcg gcgatgagga
     2641 ggcggttctg gatgacgacg ccgcagaagg cggccgccaa cccggccgtg gcgttcaaga
     2701 acgagatcag cacgggcata tcggcgccgc cgatgcggat cgcaaagagg aggccgagcc
     2761 cgaccgaggc gacgaggagg ccgagcagaa gagccatgag tgaggatccg acggcgccgg
     2821 cagccgccgc gcacaggccc gcaatcaccg ccagcagcgc gagcagcagg aggttgtggc
     2881 ggggcaggag ggtgggcgtc tgccgaagtt tggcggccag cttggcgctg gccagcatgc
     2941 tgccgctgaa ggtagcggcg ccgatgacca gcccgaggta gccagagagc tcgccgaccg
     3001 gggtgaggac gacgccggtg cgggtcaatt cgaccagggc gatggtgcag gcggccacgc
     3061 cgcccgcgcc gtgctggaag gccaccatgg ccgggatctg gatcatggtg acgcgcatgg
     3121 cgacgagggc gccgacggcg ctgccgatga gcagggccac caccagccag gccgggtcaa
     3181 agatggcgtt gcgcgagagg accagcacaa tcgcggccag cagagcggcg gcggccgtca
     3241 ggttgccgaa acgggcgccg cgcggcgtcc gaaagagggt gatgccgacg atcagcagcg
     3301 cgatgatcga gaggtccagc aaaagtgcag tgagactatt catatccata ctccattccg
     3361 tgtagaggca gccgcgccgc gtcggagcgg ggtccggtgc ggcggttcgg gccgccggcc
     3421 cgaaggccgg cgtccgtcct tccgcgtcgc ccgaaccgtt cggcgcggac cttagccgat
     3481 gggcgcggcc gccacactca ttcaggcacc gtccgttcgg ttggccggat cgtgctgcgt
     3541 cttgaacagc cgcgtcatcc ggtcggtgat cacgaagccg ccgacgaggt tgaaggcggc
     3601 ggtgatcagc gcgagcgcgc cgatcaccct ctcgcccgcg ccggacggga cagcgaacag
     3661 caggatggcc ccgagaatgg tcaccgccga cacggcattg gtcatggaca tgaggggcgt
     3721 gtggagcagc ggcggcaccc gcgagatgag caggtagccg gcgccgaacg acagggcaaa
     3781 caccagcagc atgagtatca gatccgtcat gacttcatcc tccgggcggc ggttcagacg
     3841 gtcacgcgct cgcccatggc ctccagggcg ggttcgtaga cgatccgacc gtcggttgtg
     3901 accagcgacg cgcgcacgat ttcgtcctcg aaatccggcg tccccggtcc gttcttgaac
     3961 aggttctcga tgtagtagta catgttgttc gcatagagcc aggatgactg catgggcatg
     4021 cggccgggga ggttctgaac gccgcagatc cgcacgctgt gggcccagac gtcctggccg
     4081 ggcttggtga gggcgcagtt gccgccctgg tcgatggaga tatcgatgat gaccgagccg
     4141 ggccgcatgc gggcgacgat ctcctcagtg atgatcgtgg gggccaggga gcccggcacg
     4201 agcgcggaga ggatgatgat gtcggcctgc gcggcgatag cgccgatcgc ggcctgctcc
     4261 ttgcgcagcc attcctccgg cagctgccgg gcgtatccgc cgggcgcgat agccaggtca
     4321 gccggcacgt cgaaaccctc gactttggcg ccgaggctgg cggcttcctg gcgcgcggac
     4381 tcgcggatat cccaggcttt cacgaccgcg ccaaggcgct tggcggtcgc gatcgactgg
     4441 agcccggcca cgccggcgcc gacgacgagg acggtggccg gcttgatagc cccgatcgcg
     4501 gtgccgacca tggggagaaa ccgcggaagg tactcggcgg cgtcaagcac ggcccggtag
     4561 ccggcgaccg tgctcatgga cgtcagggcg tccatgcgct gcgcgcgcga gatgcgcggg
     4621 atgccgtcca tcgtcaggga ggtgatcctc cgcgccgcca gcttgcgcac gatgtcgtgg
     4681 ctgtcgggag aggccgggtg gaggaaggtc acgagcaggg cgcgggaggg catcatgtcg
     4741 acttcatgct tctcccgggc ctcgttgaag atcggctgtt tgactttcag gatcaggtcg
     4801 gcctggccga agagttcttc gacatcgcgg acgatggcgg ctccggcctt ctcgtagtcg
     4861 ctgtcggaca cgaagatgcc ttcgccggcc ccggcttcga ccgccacctc gaagcctttc
     4921 tgacggaatt tcgcgaccgt ttcaggtatg gcggccacac gcttctcggc gtggagtatt
     4981 tctttgggga ttacgatacg catggtatcc tccggtaaac aggtttcatt tgtagatcac
     5041 gcacgttgtt cggccatcga cggcaggtcg gcggtcagac cgaggagggc atcgcgcagc
     5101 cgcccgagag cgctgcggaa ctcctcgagg tcgtcatagc gccattcgcg ctgccagatt
     5161 ttgtgcgccg tttcgtggcc cgggctgccg agaacggcgg cgccgagctt ccacggcgaa
     5221 acggtgagac agtagcggct gtcggcggcc tgggccggca gctgggcgga ctcggcgaga
     5281 tggcgcagtc cgaggtcaag gggcaggccg ttcgacgcgc gcaggtgcca ctccggattg
     5341 ggtgtgccct cggccggctc gccctcgcac cagtcgagcg acaccagcac ggcggtgccg
     5401 tcgttccaca ccggcaggac gcggtgggtc tcgatgcggc ggtcgtcatt catcaatgcg
     5461 ccggccgcgg cggcgatggc gacgacggga aggcgcgagc ccgggccggt ggcgccgttg
     5521 ctgcgccccg acggaccggc gaggtcttca aactcctgaa gcgcggtctc ctcctggtgc
     5581 gtgtacaccg ggaacttgat gatgttgggc agttcgccgg aggcgtcttg gggctctggg
     5641 gcccgggaga acgacccggg aacgctgttc ttcactccat gcgcgggaat ctcgacatag
     5701 acgggggctc cggtccacac gggaaaggcg cacggcgcca cttcggcgta gttcgcctgc
     5761 acgaccgtgt cgccggcttt gatcagcagg tgggtggacg ggccatgctg ggcgagaacc
     5821 ttcgtctcga cgtatctgcg gttcatggcg acctacctcc attgggttat gagtcagcgg
     5881 ctggtctctt cgcaacgggc gggtcgaagt gaagccggtt cggcgccgct tgtccgccca
     5941 accggccggc gcgctgccgt cagcgtcggc cctttcgacc cgtttgtttt ctctgttgag
     6001 aaataacggc agacaagaaa ttgcgacaat cgggcgccgc cggaatgtgg gggttgccaa
     6061 atcggagtcg gagcggtcat aaaggcgagc cgcgcgggaa cagaatcctg agccgacgca
     6121 acgattccgg ccacgatttc catcgcgcgg gcgaacgcct gcttcgccaa aaggagcggt
     6181 ggagctcagg gaattcctga gccggcgggc cggaaatcgc agccgtcccc gtcatcatac
     6241 gtcagccgag acgatcgttg tgaccgaggt ccgcgctcgg cggcggatat cgcggcggcg
     6301 gccgtggtgc ggcgatttcg ggcgctatcc gtaagggttt tttcaccgcg atcatcatgg
     6361 cgggaggccg cttttatctt ggcggggcga tcagcccccc gccatattgc cgccacaaaa
     6421 tgacggcgtt atcaacgacc ggcgtgatga ggaggtccta tgaagcagct aatcctgatg
     6481 gcgggagcac tattgatgct gggcatcgcc gccagcgcgc aggagtcgcc tgccggggtg
     6541 accggcaagg gtgtcaaact cggtctcgac ttttcccgaa tcaacaccga gtacgatgaa
     6601 ctggatgatt ttctggacag ccgggtgggt ttcacggccg gtgcgtttct gacctaccgt
     6661 gtcagccgcc aattcgcggt gcagccggag atcctgtacg tcgccaaggg agcggagaag
     6721 gatttcttct tctacacgcc cgaatgggct acggaatacc tggaaatccc ggtgctgctg
     6781 aagttcgacc tcatgcctgc ggggccggct catccaaacc tcttcgccgg gccggcgctg
     6841 gatttcctcc ttggttcgga gttccgggtc agcgactatc gaatcgacct ctccgattac
     6901 acccagtcgg tggacgtggg gctcgtcttc ggcggcggca tcgactacgg gtacttcact
     6961 ttcgacgcgc ggtacacggt gggattgatc ggcatggtcg atggcttcga tgatttcgcc
     7021 gccgcggtca acgaggcgac gggagcggaa ccgggcgaca gttactatct cgacggcgac
     7081 ctctcggtga agatcacgaa cacgaaccta tcgttcatgg tcggggtgag gttctgagac
     7141 ggcgccggcg ggcggggcgg aggcgggcga gccggggaga cgccggctcg gatgcggccg
     7201 cggctgtcac attctgacgc gagtccccgg cccggccggt ccggcgcccg atatcgatgc
     7261 cgcactccaa gactgctgtt gacggtgcat tgggagcggc ggatattggc ccacggaaaa
     7321 tcacgaagcg gtcggatgat tcttcgcgcc agggcccaca gcgccctggg aaaggaggcg
     7381 atgacatgag attcggtgct gctttacttc tggcggcggc ggccctgttc ggcccggggc
     7441 cggcggccgc cgacgacgcc ggtgttccgc tcacggctgt cagcgcccac tcattgggcc
     7501 ctgagcgctt ggggaatctg ccgccggtca tcgtgccggt cgacaacccc cagaccgaca
     7561 cgaaagtcct gctgggcaaa cagctctatt tcgacacccg cctgtccaag gacaacacga
     7621 tcagttgtgc gacgtgccac aatccggcca tgggttggtc ggacgcgggg ccgacgagtg
     7681 cggggatcaa cagccagcgc ggcggacgac gtgcgccgcc ggtctcgaac gcggcctaca
     7741 gcccgctgca gttctgggac ggccgggcgc cgtcgctcga agagcaggcg aaagggccga
     7801 tcgcgaaccc gatcgaaatg ggcaacaccc acgacgcgat gctccggacg gtcgacggct
     7861 tcccggagta cgtggaagag ttcaagcggg ttttcgggga ggtcccgatc acgctcgacc
     7921 tggtcgccca ggcgattgcc gcctatgagc gaacgattgt gaccaccgac tcgccgttcg
     7981 atcgcttcgt gcgcggcgat cagtcggccc tcacccggca ggagaagcag gggctggaga
     8041 tattcaacgg caaggggcac tgcacgctgt gccactgggg ggggtatttc tccgacggcc
     8101 ggttccataa tctcggggtc gccccggctc ccggcgccgc ctacgacgag ggccgccagg
     8161 cggtcacgaa agaccccgcc gacgcggcga agttcaagac gccgacggtg cgggacgccg
     8221 cgaaacgccc accctacatg cacaacggga ccgagaagac gctgcacgac gtcgtgacgc
     8281 tgtacaaccg cggcggggga acggacgatg cgaatctcga ccccctcatg ctcccgctgg
     8341 ggctgtcgcg cgcggagatc gacgccctgg tggctttcat ggagcgcgcc atggtcagca
     8401 gcaacccctc ggtggcgaac gaacagccgg tcgcgccgga gaaaatgccc aagtgaagga
     8461 ggttatcatg agcagaatga tgagacgcgg cgcgctcgcg atcggtatcc tcctgatggt
     8521 acctcaggtg agccaggggg tgccggcatt cgcgcggaag tacggtttca actgcaatat
     8581 gtgccacacg gggttcacca agctcaacga tttcggccag ctctaccgga acagcggcta
     8641 ccagattccg ggacaggagg ggttggagag aacggtgttt gagacggcgc cgccgctggc
     8701 ggtgcgcacc agtgtcggcc tgttcgccag ccaccggccc aatgccaaca ccgccgggtt
     8761 cgccatccac ggactggatg tgttctcggc cggagtgctc cacaagaaca tctcgtacct
     8821 catcgtctat acgccgcgga tcgacgaacc ggcggccgac tacaccgggc cggccgacgg
     8881 gtccgctccc agccagctcg gggggctcga atcggccaac ctggttttca gcaatctggt
     8941 gccggatgcc gtcaacgtgc gggtcggacg gttcgagccc gcctaccatc cgttcagcag
     9001 caagcggtcg tactacctgc tccagccgta cgagatttac gcgttcacca cgccgtcgaa
     9061 cggcttcgtg ttcgacgaca accagttggg agtggaggtg accgggcact tccgccgcgg
     9121 gttgttctat gccctcgggg tggtcaacgg cagcggggcc aaccccgaca acagccgcgc
     9181 gaaagacgtg tatgtgcggg cctcgcaggt gttcgggcgc ggcgaaggac agaacgccgg
     9241 ccagcgaatc ggtctgttcg ggtatttcgg gtggtgtccg accatcacgg gcgatccctc
     9301 tcccgatgcg gccgggcagg ggaatggccg ggcgcatgcg cagttccacc gcatcggcgc
     9361 cgacctcagc ctgaactggc agacgttcaa cctgcacggc atgttcctgc agggatccga
     9421 tgaccaggcc attaacctcc ccgctgtgcc cgccgacacc gcggattacg acttcaccgg
     9481 cggtttcgta cagctcgact acgccgggct gatgaacaac cggctggtcg gatcggccgc
     9541 ctacaactgg gtcacccctc ccgacttcga cgaggaatgc accattagcg cgttctccct
     9601 cctggtgcgt tattacctcg gcgactggac ggccgtcaac gtggcgctgc acgccgaata
     9661 cacccatcgc gaagaaggca aggccgataa gatcaagact gaccaactcg ggttgttggt
     9721 cgatgtcggc ctgtaagccg gtctggcgat atcgggttcg ccgacgagcg ggagcacacc
     9781 tcccgctcgt cgcattttgg cgtacgaatg cggcgaacga cccggggcgc cgccgatcgc
     9841 tgccccaact gataagacct cggttgccct ggggttcttc ggatacgcag gggaactgcg
     9901 gccgaccgcc gggatttccc gcttcagccc gtaagtgttt gccccgcacc gcacatttct
     9961 tcgacacacg catccggcgc tctccgggct ttcctccgag cgaatgcagc ttccggccgc
    10021 ccggtccgca cccccggcgc aggaaacctc ttgtcccccg agactcattc acctatcctt
    10081 ggggaaaagg tctgcaacga cacgactcga tgaagcgcta ctccgggaac ccgctgatca
    10141 gccgcactga tattccctgc atcaaaccgt cgctggtgga tgtgtcgtcg gtgttcaatc
    10201 ccggcgcggt gcggtggcgc ggccaggtgg tgctgatcct ccgggtccag aaccggggcc
    10261 gcgagacttt cctcctgacg gcgttcagcg acaacggcat tcagttccgg atcgaccgcc
    10321 gcccgatcac cttctccggc ctcgagcgct tcccgcaccg cctccaccat atctacgacc
    10381 cgcggctgac ccggatcggc gacgtgtgtt atgtcacgct ggcgatggat accgatgccg
    10441 gctgccgggt cggcctggcc cggaccacgg attttcgcgc gttcgaattc ctcggcgaga
    10501 tcctgcatga tgaggcgcgc aacgcggtga tcttcccgga gcggatcggc ggggcgtacg
    10561 cgatgctgta tcggccgaac cggacgcggc tggagggcgg ggtggccagc ggcgatgcga
    10621 tcgagtgggc ggtgtcggag aatctgcagg actggtcgcc ccgcgggccg gtgatggccg
    10681 ggcggccgca ctactgggac gagttgatcg ggtcggggcc gcccccgctc cggacccgcc
    10741 acggctggct ccacgtgtac cacggggtgg ccacccactt cgcggcgagc aacatctacc
    10801 aggccggggt cgtactgctg gccgccgacc accccggccg ggtgatcgcg cgcagccgcg
    10861 ccaatatcct ggaaccgcgg gaactctacg agctgaccgg ccaggtgccg aacgtggtct
    10921 ttccgaccgg gctgatcccg gcccgcgacg gcggcgacgg cgcggtcgag gacgacgccg
    10981 agctcctgct ctactacggg gcggccgaca cgtgcgtggg actggcgacc gcgactgtcg
    11041 gcgagttgct cgaggcgtgc gatgaagtgt gatcggtgcg aaggcgtccc ggcggcccgg
    11101 atcgccctgt ttgtgctgct gctggccgtc gtctccggat gccgaccgga gccggggcga
    11161 ggggcctcgg ccggatcgtc gggcgacact ctgccggtcc cggcgatccc ctacgcaccg
    11221 cgcggctacg tgtgctgccg gacggccgag ggcctcacgg tcgacggccg cctggaggag
    11281 gccgcgtggc ggcaggcccg gtggaccgag ctgttcacgg atatcgaggg ggaccggaaa
    11341 ccggctccgc gtttccgcac gcgcgccaaa atgctgtggg actcaacctg cttctacatc
    11401 ggggccgaac tggccgagcc gcacgtgtgg gcgaccctca cccggcgcga cgcggtcatc
    11461 taccacgaca atgatttcga ggtgttcatc gatcccgacg cggacacgca cgagtactac
    11521 gaactcgagg tgaacgcgct caacaccgtg tgggacctgc tcctgctgaa gccctaccgg
    11581 gacgggggac cggcggtcaa cgcctgggac atcgcgggtt tgcagacggc ggtggcggtg
    11641 gaggggacgc tcaacgatcc gtcggacgtc gactcggggt ggcgggtgga gatcgccatt
    11701 ccctgggcgg tcctggcgga gtgcgcgcac cggccgagcc cgccgcgcga gggggaccgg
    11761 tggaaagtca atttctcccg cgtcgaatgg caggtagagg cgcgcgacgg cgtgtatgcg
    11821 aaggtgaccg atccggcgac cggccggccg ctgccggagg acaactgggt ctggtcgccg
    11881 cagggactga tcgcgatgca ct
//
LOCUS       DAOPOE010000092        11820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6253_length_11820_cov_5.586655, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000092 DAOPOE010000000
VERSION     DAOPOE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_11820_cov_5.586655"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="PKM94_11080"
     CDS             complement(<1..2352)
                     /locus_tag="PKM94_11080"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOD67217.1"
                     /translation="MRGIKTGRAWRAGASGGAIRQVMVLAALLTLASAAHATNTNLLI
                     TTYVPDSACPGQPFEICAQLTEACGGGVSAPLPNRPVYFFLNAGSCGVGSSLIPTDSA
                     FTDSNGVACTTIPIPMANGLYSLRVKFNGEPKPANNQPPNSACDPSKRIQLSNSNDCR
                     NLVVSDSACQYPPDATCPGDTTLLLCASGEICLTGFYCSDPDDNLMACSVSLGTLTDS
                     TVCFTPTGAGVYDLVLTAMDSYLNMSSCTTSVTVVLNAAPVVDLPADTSVFLCGTTQF
                     CFPAAITDADCNVVSVTSNYGSYAGTAAGFDQIARLNEMGATVTQVGGGAPGTVLYGS
                     GDFVAPVNTQSGVAVSLPNFGFADYVADYGSFPSGLTPANSADHLLGGPTDLTFTTPG
                     IGGPDGGDGDGSVAFASGNYCVLGLYTPVTTCNGSATDFFLFTNTSSGGTIELQFLLG
                     GSPVHTVSQAIAAAANGSGFGGVTFDLPDGLTYDRVRIACLSGAPEIDGVAARTAPSP
                     TSTDVCFTATASGTYDVIVSATDACGLVGADTMTVNVLVNQPPVANAGSDQTIFLCEP
                     TEICFGVSFSDPDNNLALTEKVSGPGTLAGNEICFTPSGAGTYTFIIRAVDACGDQDY
                     DTVAVTVEGNHRPTADEPDDVVIFLCDPIQLCHTFTASDPDGDDLTWTKLAGVGTVQS
                     DGQFCFTPTTSGIYAVAAVVSDSCGLADTTSIKYTVTLNSAPVAADPGPAADLFQCDP
                     AQVCYTFAATDVNGGTLTWTKLSGAGTVSAAGQWCFTPTGAGAY"
     gene            complement(3225..4553)
                     /locus_tag="PKM94_11085"
     CDS             complement(3225..4553)
                     /locus_tag="PKM94_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD67218.1"
                     /translation="MRLCYLVLLTAFLAIPAAALAAEPATGGSADTLGPTTVETAAPA
                     LDTAAAAGAATPEYPMDPERKAKLISYSRFVNIWRFADLIITCGILALILFTGLSARM
                     RTLAQRIFRRRGLAVAGFVVLFMAVDFLLNLPFSIYRNYMVESDYGFVNQSFGGWFGE
                     AVLSLVVACVIAIIAVSFLYWVINRFRRWWLVFMIGAIPFTIFFIVIAPVLISPLFND
                     FVPIQNQGLGTKLVNLAESVGIHDPDVFQINASKQSSKINAYVTGLFGTKRIVLYDTL
                     IDNFTDDEILFVMGHEMGHYVKHHIWWGLAVALVYIGFLVWLISRIAPALIRRFQRRF
                     GFSTVGDVASLPLLILLLTVLEFGFQPITNTYSRMQEHVCDKYGVDVTGVDGETAARA
                     FDKLAVYNLSDPSPSPLVEFWFYDHPALEGRMAFVREYAAQTVKQPAVRQ"
     gene            complement(4674..5726)
                     /locus_tag="PKM94_11090"
     CDS             complement(4674..5726)
                     /locus_tag="PKM94_11090"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOD67219.1"
                     /translation="MIGKIEQLLGDQKNLLKHECKTIPKSKLHLPGPDFIDRVLVSSD
                     RSPATLRNLAAMFNHGRLAGTGYLSILPVDQGIEHSGAASFAPNPDFFDPENIVKLAV
                     EGGCNAVASTLGVLGAVSRKYAHKIPFIVKINHSEMLTYPNKFDQVMYADVEQAFDLG
                     AVAVGATIYFGAEEGMRQLQEVTAAFKYAHELGMFTVLWCYLRNPAFKKDGVDYHTAA
                     DLTGQANHLGVTIEADIVKQKSPETNAGYKAIGFGKTSEKVYTELTTDNPIDLTRYQV
                     VNCYMGRVGLINSGGESKGKGDLAAAVRTAVINKRAGGTGMISGRKAFQRPIKEGIEL
                     LNAIQDVYLEKDVTVA"
     gene            complement(5855..6664)
                     /locus_tag="PKM94_11095"
     CDS             complement(5855..6664)
                     /locus_tag="PKM94_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOD67220.1"
                     /translation="MPQHIVQVDSFTSTPFRGNPAAVCVMAGPREPEWMQAVAAEMNL
                     SETAFVHPLDGGYALRWFTPTTEVPLCGHATLAAAHVLWSEHHLNPDRQIRFHTKSGL
                     LVVTRQGELISMDFPALPVEACAPPPGLREALGLEARFVGRDKFHFLIETADEQAVRA
                     CAPDFTALARAAREGVTVTAAAGGEYDFVSRFFAPALGINEDPVTGTAHCALGPYWAG
                     KLGKPTLAGYQASRRGGAVQVTVRGERVLLAGRAVTVLRGELAAAESPRHG"
     gene            complement(6722..8998)
                     /locus_tag="PKM94_11100"
     CDS             complement(6722..8998)
                     /locus_tag="PKM94_11100"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOD67221.1"
                     /translation="MIHGRREESPRFSMRMCALCFWMCLAAMAAAAPEAPPRPTLAIA
                     RAAGPIVIDGDLSEGAWRTAANATDFTQFLPIDRVRPKSATSAYVTYDDRMLYVGFVC
                     DDDPAQVRATLTDRDRMYSDDFVGIILDTYGDAAWAYEFYVNPLGVQGDMRWLASGEE
                     DTRFDVVYYSAARVTDSGWQAEIAIPFSSLRFPDRPEQNWRATFWRSHPRQSTFKYSW
                     AAITQDMPCVLCELGYWTGIRDVKPGSRLELLPALIAYQSGGRDEDDLTTVFESGDAE
                     AEGELNVRYRLTPGLTAEATVNPDFSQVESDAAEIDVNTTYALSYPERRPFFQEGSDL
                     YGTAIPAIYTRAVNDPSIALKLTGRQGRTSFFFLAAVDDTSLQLIPLEEETEYAARGK
                     SYAAIGRVRRTFADDSYLGALFTDRRLDAGDGSGTVFGIDGALRVREKYLLSTQMLGS
                     YHRESGAPGDAGAGGEPGPAFDDEGHTVSPDGEAYSGMASYTQFQRYSRRWELEISHT
                     ATSPTFRADNGFIQRAGIQEADVEGSLSFRPNGKLVTTVTTALHVGRLWAWDGGRKDE
                     WIMPEIAFELPCQTELYGSLVLSREQFRGVWFKDIRRVEGEVSTRVLHSFRAGAAVTY
                     GNSIARMDNPVMGRELSAGFEAIIKPLQQFTIEPTWEYYRIRDRETGERLEEGYITRT
                     RFNYQFTRELFARLVVQYDDFDDRLNFEPLLTYKLNPFTIFYIGAAFRHGWDDERGID
                     GRLIQRQFFVKFQYLLRA"
     gene            complement(9136..9471)
                     /locus_tag="PKM94_11105"
     CDS             complement(9136..9471)
                     /locus_tag="PKM94_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD67222.1"
                     /translation="MKFTDTLEGDLVIIEISGKIMGGEETTMFHGRIHEYIKLNKKNF
                     LINLKKVEWMNSVGLGMLISALTTVRNAGGRLVLANIDKIESILTITRLISVFDHYDS
                     VAEARKSFA"
     gene            complement(10060..10332)
                     /locus_tag="PKM94_11110"
     CDS             complement(10060..10332)
                     /locus_tag="PKM94_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67223.1"
                     /translation="MRRRAMGFDADVAGLVGEYAGIPGGNHRAGPGSPASELAALADW
                     LVEHHAWTPSAARHLVELVARNGTFVLRNALALSIVLGVEDGELGL"
     gene            complement(11133..>11820)
                     /gene="recA"
                     /locus_tag="PKM94_11115"
     CDS             complement(11133..>11820)
                     /gene="recA"
                     /locus_tag="PKM94_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOD67224.1"
                     /translation="GEQALDITETLVRSGAVDIIVVDSVAALTPRSEIEGEMGDAHMG
                     LQARLMSQALRKLTAIISKSKTSVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRL
                     DIRRIASIKENQEVIGSRTRVRVVKNKVAPPFREAEFDIIYGKGVSREGELLDIAAAK
                     GIIEKSGAWFSYGGDRLGQGRENAKQFLADNPALFDEVYKKVQVELGISGGIGSSSGA
                     AEEEPAEVEG"
BASE COUNT         2110 a         4030 c         3575 g         2105 t
ORIGIN      
        1 gtaggcgccg gcgccggtcg gcgtgaaaca ccactgcccc gccgcactca ccgtccccgc
       61 cccgctcagc ttcgtccagg tgagcgtccc cccgttgaca tcggtggccg cgaacgtgta
      121 gcacacctgg gccgggtcgc actggaagag atcggccgcc gggcccggat cggccgccac
      181 cggcgccgaa ttcagggtca ccgtgtactt gatcgaggtc gtgtcggcca ggccgcacga
      241 atcgctcacc accgccgcca ccgcgtagat gcccgaggtg gtcggcgtga aacagaactg
      301 cccgtccgac tgcaccgtcc cgacgcccgc cagcttcgtc cacgtgagat cgtcgccgtc
      361 cgggtcggac gccgtgaagg tatggcagag ttgaatcgga tcgcagagga aaatcacgac
      421 gtcgtccggc tcgtccgccg tcggccggtg gttcccctcg accgtcacgg ccaccgtgtc
      481 gtagtcctgg tcgccgcaag cgtcgaccgc ccggatgatg aaggtgtagg tgccggcccc
      541 cgagggcgtg aagcagattt cgttgcccgc gagcgtgccg ggaccggaga ctttctccgt
      601 cagcgccaga ttgttgtcgg gatcgctgaa cgacacgccg aaacagatct ccgtcggctc
      661 gcacaggaaa atcgtctggt cgctccccgc gttggcgacg ggcggctggt ttaccagcac
      721 gttgaccgtc atcgtatccg ccccgaccag cccgcaggcg tcggtagccg acacgatcac
      781 atcgtaggtg ccgctcgcgg tcgcggtgaa acagacgtcg gtgctcgtcg gcgagggcgc
      841 ggtgcgggcg gccaccccgt cgatctcggg cgcgccgctc agacaggcga tccgcacccg
      901 gtcgtatgtg aggccgtcgg gaaggtcgaa ggtcacgccg ccgaaaccgc tgccgttggc
      961 ggccgcggcg atggcctggg agaccgtgtg caccgggctc ccgcccagga gaaattgcag
     1021 ctcaatcgtg ccgccgctgc ttgtgttggt gaacaggaag aagtccgtgg ccgatccgtt
     1081 gcaggttgtc acgggtgtgt agagcccaag cacacagtaa ttgccgctcg caaaggccac
     1141 tgaaccgtcc ccgtccccgc cgtccggtcc cccgatccct ggggtggtga aggtcagatc
     1201 tgtcggccca cccagaaggt ggtcggccga gttggcggga gtcagtcctg acgggaaaga
     1261 cccgtagtcg gccacgtagt cggcgaagcc gaaattgggc aggctcaccg cgacgcccga
     1321 ctgggtgttg accggggcca caaaatcgcc cgacccgtag agaactgtcc ccggcgcccc
     1381 gccgcccacc tgcgtaaccg tcgcccccat ctcattcagg cgggcaatct ggtcgaaccc
     1441 ggccgccgtg ccggcatacg acccgtagtt gctggtcacc gagaccacgt tgcagtccgc
     1501 atcggtgatc gccgccggga aacagaactg agtcgtgccg cagaggaaaa ccgaggtgtc
     1561 cgccggaagg tccactaccg gcgccgcatt cagcacgacc gtcacactgg tggtgcagga
     1621 cgacatgttg aggtaggaat ccatggccgt gagcacgagg tcgtagacgc cggccccggt
     1681 cggcgtgaag cagaccgtcg aatccgtgag cgtgccgagg gagacggagc acgccatcag
     1741 attatcgtcc gggtcggagc agtaaaatcc ggtcaggcag atctcacccg acgcgcagag
     1801 caagagcgtc gtatcgccgg gacaggttgc gtccggcggg tactggcagg cgctgtcgct
     1861 gaccacgaga ttccggcaat cattggagtt ggacaattga atccgcttgc tcggatcaca
     1921 ggcgctgttg ggaggctggt tgttggccgg tttcggctcc ccattgaact tcacgcggag
     1981 cgagtacaga ccgttcgcca tgggtatagg gatcgtcgtg caggccaccc cgttcgaatc
     2041 ggtgaaagcc gagtcggtgg gaatcagcga actgccgacc ccgcaactgc ctgcattcag
     2101 aaagaaatac accggccggt tgggcaacgg cgcactcacg ccgccgccgc aggcctccgt
     2161 gagctgggcg caaatctcga acggctgtcc cgggcaggcg ctgtccggca cgtaagttgt
     2221 aataagcaga ttagtgttgg tggcatgagc tgctgatgcc agcgtcaaca gcgctgccag
     2281 aaccatcacc tgccggatcg ccccacccga ggctcccgca cgccaggccc gacctgtctt
     2341 aataccgcgc atagattctc cctctgcacc tagaatatat cccgcgtcgt tcggcggaac
     2401 acgggttcag cgacgcatca ttttcaattg tgcaccggca gttcagcatt ctctttcaag
     2461 ctgcctgcta tatttcgatc tgttcgtttc ttcaaaccct gcgcctcgga gacggcatgc
     2521 cgcctccgtg tccccgttgt cgggaggtaa attgcgtggg cgatgtcagg aagggaaaat
     2581 ataccaaccg atcatcgtgt tactcccgat tacctaattt gcaccgggcc ctgagggccg
     2641 tttcgtcctt atgatgccaa tacctgggga gccagactga tataagctaa gaattaatct
     2701 gttatcagtc aagcgaaaaa gcctgttttc gcctgtccca caggcgtttt ctcacccccc
     2761 catgtcatcg aacgacgcgt acaggatctc atttgtccgc tttctccgga aacagccgcc
     2821 ggccggctgg atccgcgaca ccgggaaccg cactttccgg ggaggttcac gaggaagggt
     2881 tcccatactt cagccccctc gcacgcctgt tcccgcaatc ctctctggtg tctcctgctt
     2941 caaggcgtga gggatgggcc tcttcttgtg tgtgttcagg cgagaccact gccccagccc
     3001 cccctgcgac gccgggaaga tagctgctga agagaggccc actctggggg attcaggctc
     3061 accatccact cgggcaggaa atgttatcgt attactttac aataacataa cttttccaaa
     3121 cacgggctct cggcgagctc ctttgtagcg ccgtctgggg cttacatatt cccggcccgg
     3181 gtgcggggcc cgggagattc gtggtcggcc ggattactcg aaggttactg tcgcacagcc
     3241 ggttgcttca ccgtctgagc cgcgtactcg cggacgaacg ccatccggcc ctcgagcgcg
     3301 ggatggtcat agaaccagaa ctcgaccaac gggctcggcg aaggatcgga caagttgtac
     3361 accgccagct tgtcgaacgc ccgcgccgcc gtctcgccat ccaccccggt cacgtccacc
     3421 ccgtacttgt cgcacacgtg ctcctgcatg cgcgagtaag tgttggtgat tggctggaaa
     3481 ccgaactcca gcacggtcag caggaggatc agcagcggca gcgaggcgac atcccccacc
     3541 gtgctgaacc cgaaccgcct ctgaaaacgg cgaatcagcg caggcgcgat ccgactgatc
     3601 agccagacga gaaacccgat gtacaccagg gccaccgcca gcccccacca gatgtggtgc
     3661 ttcacatagt gccccatctc atgccccatg acgaacagga tctcgtcatc cgtaaaattg
     3721 tcgatcagcg tgtcatagag cacaatccgc ttggtgccga acagcccggt cacataggcg
     3781 ttgattttcg acgactgctt ggaggcattg atctggaaga catcggggtc gtgaatcccc
     3841 acgctctccg cgaggttcac cagcttggtc cccagcccct ggttctggat cggcacgaag
     3901 tcattgaaca gcgggctgat caggaccggc gcgatcacga tgaaaaagat cgtgaacggg
     3961 attgccccga tcatgaagac cagccaccac cgccggaacc ggttgatcac ccagtagaga
     4021 aacgacacgg cgataattgc gatgacgcac gcgaccacca ggctcaggac cgcctccccg
     4081 aaccacccgc cgaacgactg gttcacaaat ccataatccg actcgaccat gtaattgcgg
     4141 tagatgctga aggggagatt gagcaggaaa tcgaccgcca tgaacaggac gacgaatccg
     4201 gcgaccgcga gcccccgccg ccgaaaaatc ctctgcgcca gcgtgcgcat ccgggccgac
     4261 aggccggtga acaggatcag cgccagaatc ccgcaggtga tgatgaggtc ggcaaagcgc
     4321 cagatgttga caaagcgcga gtacgaaatc agcttcgctt tccgctcggg atccatggga
     4381 tactccggcg tcgccgcgcc cgccgccgcg gccgtgtcga gcgccggcgc cgctgtctcg
     4441 accgtcgtcg ggccgagcgt gtcggccgac ccgccggtcg ccggctcggc cgccagagcc
     4501 gccgccggaa tcgccaagaa cgcagtgagg agaaccagat agcacagacg cattgtcgtt
     4561 tacctccggg tgagaatcgt aatgcaggag tatacgggac aacggcggcc ggggtgcaaa
     4621 aaaaaagagg cgggcatcca cgacccgcct cccggtattc gccgccgggc ggcctacgcc
     4681 accgtcacgt ccttctccag gtagacgtcc tggatcgcat tgagcagctc gatcccttcc
     4741 tttatgggcc gctggaacgc tttccggccg gagatcatgc cggtcccgcc ggcgcgcttg
     4801 ttgatcaccg ccgtgcgcac tgcggccgcg agatccccct tccccttcga ctcgccgccg
     4861 gagttgatca ggccgacccg ccccatgtag cagttgacca cctggtaccg ggtcagatcg
     4921 atcgggttgt cggtcgtcag ttcggtgtag actttctcac tggtcttccc gaacccgatc
     4981 gccttgtagc cggcgttggt ctccggcgac ttctgtttga cgatgtccgc ctcgatcgtc
     5041 accccgaggt ggttcgcctg gccggtgaga tcagccgccg tgtggtagtc gacgccgtct
     5101 ttcttgaacg cgggattgcg caggtagcac cagagcacgg tgaacatccc gagctcgtgg
     5161 gcgtacttga acgccgccgt cacctcctgg agctggcgca tcccttcctc ggccccgaaa
     5221 tagatggtcg ccccgaccgc caccgccccc agatcgaagg cctgctcgac atcggcatac
     5281 atcacctggt cgaacttgtt ggggtaggtc agcatctcgc tgtggttgat cttgacgata
     5341 aacgggatct tgtgcgcgta cttgcgcgac accgctccga ggacgccgag cgtggaggcc
     5401 accgcgttgc accccccctc caccgccagc ttcacaatat tctcggggtc gaagaaatcc
     5461 gggttggggg cgaacgaggc cgcgccggag tgttcgatcc cctggtcgac cggaaggatc
     5521 gagaggtacc cggtgccggc cagccgcccg tggttgaaca tggccgcgag attgcgcagg
     5581 gtggccggac tgcggtccga cgaaaccagg acccgatcga taaaatcagg gccgggcagg
     5641 tgcagtttcg acttcgggat cgtcttgcac tcgtgcttca acagattctt ctggtcgccc
     5701 agaagctgct cgattttgcc tatcatgata ccactctcct atgttaacag ccgcgggtac
     5761 ggcgttgacg aatctgtacg taccgctcaa aactactgtc acacccgctg tttctccaag
     5821 ttaaaagctc ggcggccgcg ccggcgacgg tcggtcaccc gtgccgcggc gattcggcgg
     5881 ccgccagttc cccgcgcagc acggtcaccg cccgccccgc caggagcacg cgctcgccgc
     5941 gcaccgtcac ctgcaccgcc cccccacgcc gcgaggcctg gtaacccgcc agcgtcggtt
     6001 tgcccagttt ccccgcccag tagggaccga gtgcgcagtg cgccgtcccg gtcaccggat
     6061 cctcgttgat ccccagggcc ggcgcgaaaa accgcgagac gaagtcgtac tccccgccgg
     6121 ccgcggcggt caccgtcacc ccttcccgcg ctgcccgcgc cagggcggtg aagtcgggcg
     6181 cacaggcgcg caccgcttgc tcatcggccg tctcaatcag gaaatggaac ttgtcgcggc
     6241 cgacaaagcg cgcctcgagc cccagggcct cgcgcagccc cggcggcggc gcgcacgcct
     6301 ccaccggcag cgccgggaaa tccatgctga tcagctcccc ctgccgggtg accacgagca
     6361 agccgctctt ggtgtgaaac cggatctgcc ggtcgggatt gaggtggtgc tcgctccaca
     6421 gcacgtgggc ggccgccagc gtcgcatggc cgcacagcgg cacctcggtc gtgggcgtga
     6481 accagcgcag cgcatacccg ccgtcgagcg ggtgcacgaa ggccgtctcc gagaggttca
     6541 tctcggccgc caccgcctgc atccactccg gctcccgcgg gcccgccatc acgcacaccg
     6601 cggccgggtt gccgcgaaac ggcgtgctcg tgaagctgtc cacctgtacg atgtgctggg
     6661 gcatacaggc aaggtatagc ccgttggccg ccgcggtcaa gggcgaactc ccccgcggcc
     6721 gctacgcccg gaggaggtac tggaacttga cgaagaactg ccgctggatg aggcgcccgt
     6781 cgatcccccg ctcgtcgtcc cacccgtggc ggaacgccgc cccgatgtag aagatggtga
     6841 aggggttgag cttgtaggtc aggagcggtt cgaaattcag ccggtcgtca aaatcgtcgt
     6901 actgcacgac cagccgcgcg aacaactcgc gcgtgaactg gtaattgaag cgcgtccgcg
     6961 tgatataccc ctcctcgagg cgctcgcccg tctcgcggtc ccggatccgg tagtattccc
     7021 aggtcggttc gatcgtgaac tgctgcagcg gcttgatgat cgcctcgaat ccggccgaca
     7081 gctcccgccc catcaccggg ttgtccatcc gggcgatcga attgccgtac gtcaccgccg
     7141 cgccggcgcg gaaactgtgc agcacgcgcg tgctcacttc cccctccacc cgccggatgt
     7201 ccttgaacca cactccccgg aactgctcgc ggctgagcac cagcgatccg tacagctcgg
     7261 tctggcaggg caactcgaag gcaatctccg gcatgatcca ctcatccttc cgcccgccgt
     7321 cccacgccca caggcggccc acgtggaggg ccgtggtgac tgtcgtcacc agtttcccgt
     7381 tggggcgaaa cgacagactg ccctcgacat ccgcctcctg gatgccggcg cgctggataa
     7441 acccgttgtc cgcccggaaa gtcgggctgg tcgccgtgtg gctgatctcc aactcccacc
     7501 gccgggagta gcgctggaac tgggtgtagg aggccatgcc gctgtaggct tccccgtccg
     7561 gcgacaccgt gtgcccctcg tcgtcaaacg ccggccccgg ttccccgccc gcccccgcgt
     7621 ctcccggcgc ccccgattcc cgatggtagc ttcccagcat ctgggtcgag aggaggtatt
     7681 tttcacggac gcgcagggcg ccgtcgatcc cgaacaccgt ccccgaaccg tccccggcgt
     7741 cgagccgccg gtcggtgaaa agcgcaccga ggtaggagtc atcggcaaag gtgcgccgca
     7801 cccggccgat ggcggcgtac gatttgccgc gcgccgcata ctcggtctcc tcctcgagcg
     7861 gaataagctg cagcgaggtg tcgtcgaccg ccgcgaggaa gaagaaactt gttcgcccct
     7921 gccgcccggt cagtttcagc gcgatgctcg ggtcgttgac cgcgcgcgtg tagatcgccg
     7981 ggatcgccgt gccgtacaga tcgctcccct cttgaaagaa cggccgccgc tccggatagg
     8041 acagggcgta ggtggtgttg acgtcgatct cggccgcatc cgactccacc tggctgaaat
     8101 ccgggttcac cgtcgcctcg gccgtcagcc cgggagtcag gcggtagcgc acgttgagct
     8161 ccccctccgc ctcggcgtcg ccactctcaa acaccgtcgt gaggtcgtcc tcgtcgcggc
     8221 cgccgctctg gtaggcgatc agcgccggca aaagttcgag gcggctgccc ggcttgacat
     8281 ccctgatccc cgtccagtag cccagctcgc acagcacgca cggcatgtcc tgcgtgatgg
     8341 ctgcccagga gtatttgaac gtgctctgcc gcggatggct gcgccagaat gtcgcccgcc
     8401 agttctgctc gggtcggtcg ggaaaacgca gcgagctgaa cgggatcgcg atctccgcct
     8461 gccagccgga atcggtgacg cgggcggcgc tgtaatagac gacgtcgaag cgggtgtcct
     8521 cctcgcccga ggccagccag cgcatatccc cctgcacgcc gagcgggttg acgtagaact
     8581 cgtaggccca cgccgcatcc ccgtaggtat cgaggatgat ccccacgaaa tcgtcgctgt
     8641 acatccggtc gcggtcggtc agggtggccc gcacctgcgc cggatcgtcg tcgcacacga
     8701 aacccacgta cagcatccgg tcgtcgtagg tgacatacgc cgaggtggcg gatttcggcc
     8761 gcacccggtc gatcggcagg aactgggtaa aatcggtggc gttcgcggcc gtccgccacg
     8821 ctccctcgga caggtcgccg tcgattacga tcggccccgc ggcgcgcgcg atcgccaggg
     8881 tcggccgcgg gggcgcctcc ggcgcggccg ccgccatcgc ggccagacac atccagaagc
     8941 agagcgcgca cattcgcatg ctgaacctcg gcgactcctc gcggcgaccg tggatcatac
     9001 cgtcgccgtt accttccgta tacggcggcc ggggggcgcc ggttgccggc ccgccggtcg
     9061 ctcacggagc accggacaac aaaagggcct cgcacggcaa ggccctctcc accgtcgacc
     9121 atggcgccgg cgctctcagg caaatgattt cctggcctcg gccacgctgt cgtagtggtc
     9181 gaacaccgag atcagccggg tgatcgtcag gatcgattcg atcttatcga tgttcgccag
     9241 caccagccgc ccgcccgcgt tccggaccgt cgtcaacgcc gaaatcagca tccccaggcc
     9301 caccgagttc atccactcga ccttcttcag gttaatcagg aaattcttct tgttcagctt
     9361 gatgtactcg tggattctcc cgtggaacat ggtcgtctct tccccgccca tgattttgcc
     9421 ggaaatttcg atgatgacca gatccccttc gagcgtgtcg gtaaacttca tagcccgatc
     9481 ctcagtctgt atatagtggt ttgctgtcgt ccgtcaccta tcggccgctc tcgtccgggt
     9541 cctggggcag gtcggagaga ggcacgcgat caccctgccg gaactgcatc cccggtccga
     9601 tcaaaatacc ggccgcccaa ccaaacgcaa gcggtttttg ggcgccgccc gcccgctcgt
     9661 tttccgcttg cgcaaacggg ccgggcaggt tatactaagg cgattgtaaa tggcagcatc
     9721 actctacgcc acgaggtcct ttcgatcttt cgctaacgga ctgtcacgtg cggttctgaa
     9781 cgtcgacggg gaatagctta tgcgtccgtt acgctcccgc cgtaatcccg gcgctgtcgc
     9841 ccggccgccg agcggtgttt cgccgcggcc gcgcaagccc gaccgttccg gttgacccgc
     9901 cgacatccgg tcgccggccc gcgagcccgt gcggcattcg gggcgaggtc gggacggcga
     9961 tggaggaaaa ttcccggccg ctgagcccgg tcgggaacgc aacgcaaagg ccgctccgaa
    10021 ggatccggag cggcctgctt tatgcttgcg cctttccggc tagaggccca actcgccgtc
    10081 ctcaacaccc aggacgatcg agagcgccag agcgttgcga aggacgaacg tgccgttccg
    10141 ggcgacgagt tcgacgagat ggcgcgcggc cgaaggtgtc catgcatggt gctccaccag
    10201 ccaatcggcg agtgcggcaa gctcagacgc aggcgagccc ggaccggctc gatggttccc
    10261 tcccggaatg cccgcgtact ctcccaccag accggctacg tcggcatcaa accccattgc
    10321 tctcctcctc actgtcctcg gaacactttc tggcgatgcc actccaatcg caccggtgac
    10381 ggggcaaatc tccgcggcgg ggcgattggg cggccgtgat aaaccccgac gagctgccgc
    10441 cctcggcgat cgagcccctc ccggaggctt gcggcaagaa cgacgcggag atcagtgtcg
    10501 acagtcatga agccccggtc aaagagcctg tcaaaggtcg cgctgagaca gagcccgttg
    10561 tggggatcgg cacgtccctc ttcagcgaga ctccacggaa ctatgtggct cgcgattaga
    10621 cactcccgaa ttctcagtcc ggtaacgcag caccgctcat cataggcgct caggacggcc
    10681 tcgcggaaaa agtcctggcc gaccctcacc tttctggagg ccagagtctc tgtcggcccg
    10741 ctgcgcaatg tttccgcgct ctgccccacc tcttggatcc gtcgctcgcg gagttgactt
    10801 gcctgcagga cgagttcact ccaattggag tggaactctc gccagaccgc tcgatccaaa
    10861 tccccggtgt gcggcaggcc cccgatctgg cgtttccgaa gggccgggtc aagcgatcca
    10921 aaattcccca gtttccgggc gacgccggcc ggtgaccgcc ccagcaaccg cgctaattca
    10981 acgacgaggg gattgcccgc tttcgtgttc gcaaacggga tgcggcaata gagatcgaaa
    11041 gcaagaatca actcctcccg gtcccaggga gccccatacc gtggaggtct ctggtcctct
    11101 cccatgcctg tgcccgctcc ggcgtttccc cctcagccct ctacttccgc cggctcctcc
    11161 tccgccgccc ccgaggaact cccgatcccg cccgaaatgc ccagctccac ctgcaccttc
    11221 ttatagacct cgtcgaacag cgccgggttg tccgccaaaa actgcttcgc gttttccctt
    11281 ccctggccga gccggtcgcc gccgtaggag aaccaggcgc cggatttctc gatgattcct
    11341 ttggcggccg cgatatcgag cagctcccct tcgcgcgaca cccccttgcc gtaaatgatg
    11401 tcgaactccg cctcgcggaa cggcggcgcc accttgttct tgactacccg cacgcgcgtg
    11461 cgcgacccga tcacctcctg gttctccttg atcgaggcga tccgccggat gtcgagccgc
    11521 accgaggcgt agaatttcag cgcgttcccg cccgtcgtcg tctcgggatt gccgaacatg
    11581 accccgattt tcatgcggat ctggttgatg aagacgacgc tcgttttgga tttgctgatg
    11641 atcgcggtga gtttgcgcag cgcctgcgac atgagccgcg cctgcagccc catgtgcgca
    11701 tcccccatct ccccctcgat ctccgagcgc ggcgtgagcg cggccaccga atcgaccacg
    11761 atgatatcca cggcgcccga ccgcacgagc gtctcggtga tgtcgagcgc ctgctcgccc
//
LOCUS       DAOPOE010000093        11741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6311_length_11741_cov_5.774517, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000093 DAOPOE010000000
VERSION     DAOPOE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_11741_cov_5.774517"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..1931)
                     /locus_tag="PKM94_11120"
     CDS             complement(357..1931)
                     /locus_tag="PKM94_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOD67225.1"
                     /translation="MARERWGTKIGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIP
                     YFVALVLLGIPLMWVEWGMGRYGGRFGHGATPGQFHAMWQHPISKYLGALGLFLSLAT
                     MIFYVYVESWSLGFSFFSITKSYFGMESFDSMRHFLSSYQGVTEGFFTSIWPAYLILL
                     LTLAINFYVVYHGIAKGIERLALIAMPALILFAIILVVRVVTLGTPDPVAHPDWNVES
                     GFAFIWNPDLSQLGSAKVWLAAAGQIFFTLSLGSGLIQTYSSYVRENDDIALGGLTTA
                     SINEGVEVVLGASIAIPIAAAFFGIAVTQEIAARGAYDLGFVSLPIIFTKIPAGSIFG
                     CLWFLLLFFAGITSSVAMAQPLIAFLKEEFRFSHRKAVAVLAALVLIPIHFVVFFLGK
                     GYLDEMDYWVGTFGLVVFALIETIVFAWVFGMDKGWAEITRGAEVRVPRIFYYVIKYV
                     TPVYLLVIMIYWIVLDAVPTLLMRGGNPENVPYRWLSRAVMLLLFVVMIYLVRRAWRR
                     NDVVKSFERPDEEAAV"
     gene            complement(2215..2565)
                     /locus_tag="PKM94_11125"
     CDS             complement(2215..2565)
                     /locus_tag="PKM94_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOD67226.1"
                     /translation="MKTYVVVSKISQHGPSLVEVAAKMQSLDASGRQWIQDVQQHCPE
                     VRFLAHYALLGPYDFLDIYEAPDEESAAKVSLLASADRMFTVESWTAIPDKRLAEVLR
                     ALRRRRTDGDPKTA"
     gene            complement(2915..6571)
                     /locus_tag="PKM94_11130"
     CDS             complement(2915..6571)
                     /locus_tag="PKM94_11130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOD67227.1"
                     /translation="MKIQRYFTRDGESPYSRIPFAKRSSRIVNPDGSVVFELKDIHVP
                     EAWSQVAVDILAQKYFRKAGVPLRDEDGRPVLDEKGQPKTGGETDSRQVFHRLAGCWR
                     YWGERYGYFSGEADARAFAEELSYMLAAQIAAPNSPQWFNTGLNWAYNITGPAQGHYY
                     VDEETHTLTETKSAYERPQPHACFIQSINDDLVNEGGIMDLWVREARLFKYGSGTGTN
                     FSRLRSLGEPLSGGGCSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVTLNLDHPDIQA
                     FIAWKVTEEQKVATMVAGSRIIRDQLRAIARAARGAGSNGAAACETDPEKNAALKEAL
                     RTARRFAVPQAYIKKVLDLLRQGISDIDFIELDTNWEGEAYATVSGQNSNNSVRIPNR
                     FFEKLKEGGNWELIRRTDNQTARTVPAAELWEEICYAAWSCADPGVQFDTTINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASINLVKFVRDDGSFDIEGFFHAVRLWTVVLEI
                     SVLMASYPSRPIAQRSYEFRTLGLGYANLGTLLMRLGIPYDSPEAYTWCGVITAMLTG
                     QAYVTSAEMARELGPFPGFWRNREHMLRVIRNHRRAAYNADKAEYEGLTIKPIGINGT
                     YVPEELVHAARRVWDRALEMGETHGYRNAQVSVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINNSVPIALHRLGYTERQIADIINYATGHKTLKGAPFINHESLQA
                     KGFGDREIEKIEEGLGDVFDISFAFNKHTLGEDFLRETLNLPPEHYNDFSFNLLRDLG
                     FTREQIDAANEYCCGTMTLEGAPQLDEKHLPIFDCANRCGKKGKRYISFEAHIRMMAA
                     AQPFISGAISKTINMPAEAALEDVKRAYRLAWETMNKAIAIYRDGSKLSQPLSASLLG
                     EDEEEEAPQLAVEILAEQAARKLVYRYIAKRRKLPNRRAGYTQKAVVGGHKIYLRTGE
                     YADGTLGEIFVDMHREGAAFRSLMNSFAIAVSLGLQHGVPLEEFVEAFLFSRFEPNGF
                     VRGNHAIKRATSIIDYIFRELAITYLGRTDLAQVSDDDLRHDAIGVPADETAEFIEEK
                     AVDYGASGSRKSDDIHSDHIEIDLGGNGHGNGNGHKPDNHSATGGNGNGKSLAGAAAA
                     AHPAEETVLEYVAPAGGTPAGGTQAQRLQREIRAARLKGYEGDMCAECGQFTMVRNGT
                     CLKCDTCGATSGCS"
     gene            7429..8010
                     /gene="pgsA"
                     /locus_tag="PKM94_11135"
     CDS             7429..8010
                     /gene="pgsA"
                     /locus_tag="PKM94_11135"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOD67228.1"
                     /translation="MPNRLTLLRVIIAPIFMYFFLLDSYWMKLTALVLFVVAALTDFA
                     DGYYARKYGIITGFGKFMDPLADKVLVSSALITFIALDVVSPLPVILIVGREFLITGL
                     RMLAAYRGVVIPPTWWAKVKTFLQLSVVGLVLAYINLILTLEHFDSPARPYFQFDYPF
                     YFNILLWITAAVTVWTGIDYIRKYYYMIKTVLK"
     gene            8021..8485
                     /locus_tag="PKM94_11140"
     CDS             8021..8485
                     /locus_tag="PKM94_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOD67229.1"
                     /translation="MKRGLVQLIAGGLYSGASPIVPGTTGSIPPWLIAWFLLAGNQAA
                     LGIVAVLTVFVSVWAAGEAERFYGHDAKKIVIDEWAGMFLTLLFVPYSLVNYLIGFAA
                     FRAFDVVKIPPAAQAERLPRGWGVTMDDVVAGVQANILTQIVVRLIARSAGA"
     gene            8504..9751
                     /locus_tag="PKM94_11145"
     CDS             8504..9751
                     /locus_tag="PKM94_11145"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOD67230.1"
                     /translation="MPMCAEIITVGDELITGHRIDTNSAFIAQQLTRIGLEVRYRSSV
                     GDSLEMMEEVFRLALKRAQVVIVTGGLGPTDDDITKKAIVRVFKRNLIFHEELLEELK
                     KRYAARGIEMPAINQNQALLPQGATVFPNKYGSAVGIGIAEGGTVFLALPGVPYEMKQ
                     IMTDEIIPYLQRLNIGQVLEVVSLRTTGAVESKLAELIAPGLKLEPGVKLAYLPAPSA
                     IELRVLAAAATREEAQEKAQAVVRYLESTVGKYIFGRGDDTLEGVVGQLLVDNDKSLA
                     VAESCTGGELGMLITSVPGASRYFLGGVVAYSNEAKTELLGVDRDIIAQFGAVSRECA
                     EAMAEGCRRRFGADYALAVTGIAGPGGATPDKPVGTVWIGLASLHETYAKTFSLGTLR
                     ESIRDRAAYTALEMLRRNILDIT"
     gene            9751..10329
                     /gene="thpR"
                     /locus_tag="PKM94_11150"
     CDS             9751..10329
                     /gene="thpR"
                     /locus_tag="PKM94_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOD67231.1"
                     /translation="MRLFIALPPAEAVAEELGRVIVLLRAKGGPVRWVAPENIHLTVR
                     FLGETDEARVPKLRSLIDSIAPAFAPADVLIDRLGGFPNLRKPRVIWAGLAAAEPVER
                     MAQMARQVELAVRQLRFEPEKKGFQPHLTLGRVKDPRGMEQLAAFMESYRFTPLAVRL
                     DRLVLFRSTLTPKGSIYERVHEAKLGDERLGG"
     gene            10335..10790
                     /locus_tag="PKM94_11155"
     CDS             10335..10790
                     /locus_tag="PKM94_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX pyrophosphatase"
                     /protein_id="HOD67232.1"
                     /translation="MRRPIQVLIFVYRQTARGREYLMLRRIPELRGFWQPISGGVEDD
                     EDLLTAARRELREETGFQTAAVESINYAYRIPAFHDRKLGAAPGADETMPLHTFAARV
                     DDGAEPVLDPAEHDRYLWCPLLLADRLLYWPGDREALRQVDTWLRSRPL"
     gene            complement(10841..>11741)
                     /locus_tag="PKM94_11160"
     CDS             complement(10841..>11741)
                     /locus_tag="PKM94_11160"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD67233.1"
                     /translation="DPAGVAFTADPQLGDAPLAVQFTGTSLLDVDTWAWSFGDGDSAF
                     VQSPGHVYAAPGLYDVSLQVTAGAESARRERADYIAVLADTLRADDRSVPRFSEVELA
                     VFAANNLPLSEIRIPVTFAGTLDLTLDSLTTSGCRTDYFAVRGFIDLNNSLKQGTVQL
                     VSAPSGSSPLPPGSGPVVRLWFTTAGAASGDSAVVSFNGYGSFTPRFVSSIAAYAPKT
                     VSPVLVYQNCCVGTRGDVDNSGSLNVTDLTFLISYLFRGGPEPACYEAGDVDLSGIVA
                     VADVTYLIAHLFRGGSAPYPCSS"
BASE COUNT         2042 a         3716 c         3885 g         2098 t
ORIGIN      
        1 ggactgcaag acctgggcgt cccccgtgaa ttctatctcg cacacggccg aaccgtccgg
       61 cccgaccgtg atcgtctctt tccgctccag gcaggcgctg cagcccaggg ccagcgccgc
      121 ggccgccgcg ggcagcaggc gtctcaggat tggtgtcgtc atgggatcct ccggttttca
      181 ggtttctgcc ggtaacgaca ccgcacccgg cgaaaacgtt gacgcggacg gccgaaaaaa
      241 acgctagtcc ggcgagcccg ccttcttcct caacacccgc gccaggcaaa aggccgacag
      301 ggacaggatg atgccccagg cgaccgcgag gaagattatt ccgccgacgg tgaggttcat
      361 accgcggcct cctcatcagg ccgttcaaag gatttgacca cgtcgttccg ccgccaggcc
      421 cggcgcacga gatagatcat cacgacaaac agcagcagca tgaccgcgcg ggacagccac
      481 cggtagggga cgttctccgg attgccgccg cgcatgagca gggtcgggac ggcgtcgagg
      541 acgatccagt atatcatgat gaccagcagg tagacgggcg tgacgtactt gatcacgtag
      601 tagaagatgc ggggaacgcg gacctccgcg ccgcgcgtga tttccgccca gcctttgtcc
      661 atcccgaaaa cccaggcgaa tacgatcgtc tcgatgaggg cgaacacgac cagtccgaac
      721 gtccccaccc aatagtccat ctcgtccagg tagcccttgc cgaggaagaa gacgacgaag
      781 tgaatcggga tgagcacgag ggcggccagc accgccaccg ccttgcggtg actgaatcgg
      841 aattcctcct tgaggaaggc gatgagcggc tgggccatcg ccaccgacga ggtgatgccg
      901 gcaaagaaga gcaggaggaa ccagaggcag ccgaagatcg atccggccgg gattttggtg
      961 aagatgatcg gcagcgagac gaaaccgaga tcgtaggcgc cccgggccgc gatctcctgg
     1021 gtgacggcga tgccgaagaa ggcggccgca atggggatgg cgatgctcgc gccgagcacc
     1081 acctccaccc cttcgttgat ggacgcggtc gtgagaccgc cgagcgcgat gtcgtcattc
     1141 tcgcgcacat agctcgagta cgtctggatg agccccgagc cgaggctcag ggtgaagaaa
     1201 atctgcccgg cggcggcgag ccagaccttg gccgagccga gctgcgagag atcgggattc
     1261 cagataaagg cgaagccgga ttcgacgttc cagtccgggt gcgccaccgg gtcgggtgtc
     1321 ccgagagtga cgacgcggac gaccaggatg atcgcgaaga gaatcagggc cggcattgcg
     1381 atcaaggcca gccgctcgat ccccttggcg atcccgtggt agacgacgta gaagttgatc
     1441 gccagggtga gcagaaggat caggtaggcg ggccagatgc tcgtaaagaa cccttccgtc
     1501 accccttggt agctcgacag gaagtggcgc atcgagtcga acgactccat gccgaagtac
     1561 gacttggtga tcgagaagaa actgaacccg agcgaccacg attcgacgta cacgtagaag
     1621 atcatcgtgg ccagcgagag gaacaggccg agggcgccga ggtacttgga gatcgggtgc
     1681 tgccacatgg cgtggaactg gccgggggtc gcgccgtgcc cgaaccggcc gccgtagcgc
     1741 cccatgcccc actccaccca catgagggga atgcccagca ggacgagggc gacgaagtag
     1801 gggatcatga acgccccccc gccgttctcc gccgcctgga cggggaaacg caggaaattg
     1861 cccaggccga ccgcgttacc ggccatggcc aggatgagtc cgattttggt tccccagcgc
     1921 tctcgtgcca tcgctctacc tccatcggtc ccgcgcggcg gaaccgaagt gcgtgaaagg
     1981 tgcgtgtgat ctggctgtga aagatgtggg gcggtgcagc ggcaaccgcc tgacttacga
     2041 aggcatccgg cccctcaggc cggaatgcga ctatatatca tgggggggag ggggcgcgca
     2101 agcacgaagt ggccgcccgg gttgccgcgc gggcgctttg gcgccgcgcc gcgcaccctg
     2161 aggactccgg gcgccccggg gcggacgccg cacgcgatct gtcttggtcc cggttcaggc
     2221 ggttttcgga tccccgtccg tccgccgccg gcggagcgct ctgagcacct cggccaggcg
     2281 cttgtccggg atggccgtcc agctttccac cgtgaacatg cggtcggcgc tggccagcag
     2341 cgacaccttc gccgcgctct cctcgtcggg cgcctcgtaa atatcaagga aatcgtacgg
     2401 tccgaggaga gcgtagtggg cgaggaaacg gacctcgggg cagtgctgct ggacatcctg
     2461 tatccattgg cgccccgacg catccagcga ctgcatcttg gcggcgactt ccaccaggct
     2521 cggcccgtgc tgggaaatct tcgacaccac gacataggtc ttcatgagaa cctccgatcg
     2581 gtgaaggctt tcgatggcgc gcgcactgcg ctgccccggc ggggggagat caccgccgcc
     2641 cccgccgtct gtgagtaata tacggaggaa cggggagcgg gggaacgtca aagtggcaga
     2701 aaaatatgcg cccggcgggc agggaaccag gaaagcgcag ggcgcgtgtg tatggtcggc
     2761 ctctggcaag aggtgcgatg tgtgcgattt ctcagcggcg cccgggcgag gggcccgggc
     2821 cgccgctgct ttcatccctg aaaggatccc tgatccatca ggtccacggg acacttttct
     2881 cggacgcggg ccgaagcccg gcgcggccta ctcctcagga gcagccgctc gtggcgccgc
     2941 aggtatcgca cttgagacag gtgccgttgc ggaccatggt gaactggccg cactcagcgc
     3001 acatatcgcc ttcgtaaccc ttaaggcgcg ctgcgcggat ttcgcgctga agtctctggg
     3061 cctgggttcc ccccgcaggc gttccacctg ccggggcgac atactcaagc accgtctctt
     3121 ctgctgggtg cgccgctgca gccgcacccg ccagcgattt accattgccg ttccctccgg
     3181 ttgcgctgtg attgtccggc ttgtggccat tgccgttccc gtggccgttg ccgccgagat
     3241 cgatctctat gtggtcggag tggatgtcgt ccgacttgcg cgagccgctc gccccgtagt
     3301 cgaccgcctt ctcctcgatg aactcggcgg tctcgtcggc cggcacgccg atcgcatcgt
     3361 ggcgaagatc gtcgtcggat acctgggcga ggtcggtgcg cccgagatag gtgatggcca
     3421 gttcgcggaa aatatagtcg atgatcgagg tggcgcgctt gatcgcgtgg ttgccgcgca
     3481 cgaacccgtt gggttcgaac cgcgagaaga ggaaggcttc gacgaactcc tcgagcggca
     3541 cgccgtgctg cagcccgagg gagacggcga tagcgaagct gttcatgagg gagcggaagg
     3601 cggccccttc gcggtgcatg tcgacaaaaa tctcgccgag cgtcccgtcg gcgtactcgc
     3661 ccgtgcgcag gtagatcttg tggccgccca ccaccgcttt ctgcgtgtac ccggcgcggc
     3721 ggttggggag tttgcggcgc ttggcgatgt agcggtagac cagcttgcgc gccgcctgct
     3781 cggcgagaat ctccactgcc agttgcggcg cctcctcctc ctcgtcctcc ccgagcagcg
     3841 aggccgagag cggctggctg agtttggagc cgtcgcggta aatcgcgatc gccttgttca
     3901 tcgtctccca cgccagccgg taggctcgct tgacgtcctc gagggcggcc tcggccggca
     3961 tgttgatcgt cttggagatg gcgccggaga tgaacggctg ggcggccgcc atcatccgga
     4021 tgtgcgcctc gaagctgatg tagcgtttcc ctttcttgcc gcaccggttg gcgcagtcga
     4081 agatggggag gtgtttctcg tcgagttgcg gcgccccctc gagcgtcatc gtgccgcaac
     4141 agtactcgtt ggcggcgtcg atctgctcgc gggtgaagcc gaggtcgcgc agcaggttga
     4201 agctgaagtc gttgtagtgt tcggggggca ggttgagcgt ctcgcgcagg aaatcctcgc
     4261 ccagggtgtg cttgttgaag gcaaaggaga tgtcgaatac gtcccccagc ccctcctcga
     4321 ttttctcgat ctcccggtca ccgaacccct tggcctggag cgactcgtgg ttgatgaagg
     4381 gcgccccctt gagagtcttg tggccggtcg cgtaattgat gatgtcggcg atctggcgct
     4441 ccgtgtagcc cagccggtgc agggcgatgg ggaccgagtt gttgatgatc ttgaaatagc
     4501 cgccgccggc caacttcttg aatttcacga gcgcgaaatc cggctcgatc ccggtcgtgt
     4561 cacagtccat gaccaggccg atcgtcccgg tcggcgcgat caccgacacc tgggcgttgc
     4621 ggtagccgtg cgtttcgccc atctccagcg cccggtccca gacgcggcgg gccgcgtgca
     4681 ccagctcctc cggcacatag gtgccgttga tgccgatcgg cttgatcgtc aaaccctcgt
     4741 actccgcctt gtcggcgttg taggccgccc gccggtggtt gcggatcacc cgcagcatat
     4801 gctcgcggtt gcgccagaag ccggggaagg ggccgagctc ccgggccatc tcggccgacg
     4861 tcacataggc ctgcccggtc agcatggccg tgatcacccc gcaccaggtg tacgcctcgg
     4921 gcgaatcgta ggggatgccc agccgcatga gcagggtgcc gagattggcg tagcccaggc
     4981 cgagcgtgcg gaattcatag ctccgctggg cgatcggccg cgacgggtac gaggccatga
     5041 gcaccgatat ctccagcacc accgtccaca gccgcacggc gtggaaaaag ccctcgatat
     5101 cgaacgagcc gtcatcccgg acgaatttca cgaggttgat cgaggccagg ttgcacgcgg
     5161 tgtcgtccag gaacatgtac tccgagcagg gattggaggc gttgatccgg ccgtcttccg
     5221 ggcaggtgtg ccactcgttg atggtcgtgt cgaactgcac ccccgggtcg gcgcacgacc
     5281 acgcggcgta gcagatctcc tcccagagct cggcggccgg gacggtgcgc gccgtctggt
     5341 tgtcggtgcg ccggatcagc tcccagttcc ctccttcttt gagtttctcg aagaagcggt
     5401 tggggatgcg gaccgagttg ttcgagttct ggccggagac cgtggcgtag gcctccccct
     5461 cccagttggt gtcgagctcg atgaagtcga tgtcgctgat cccctgccgc agcaggtcga
     5521 ggactttctt gatgtaggcc tgcggcacgg cgaagcggcg ggcggtgcgc aacgcctctt
     5581 tgagcgccgc gttcttctcg gggtcggtct cgcacgcggc ggcgccgttc gaaccggccc
     5641 cgcgagcggc ccgggcgatc gcccggagct ggtcgcggat aattctcgac cccgccacca
     5701 tggtcgcgac cttctgctcc tcggtgactt tccaggcgat gaaggcctgg atgtcggggt
     5761 ggtcgaggtt aagggtgacc atcttggcgg cccgccgcgt ggtgccgccg gacttgatgg
     5821 cgccggccgc ccggtcgccg atcttcagaa acgacatgag ccccgaggag cacccgccgc
     5881 cggagagcgg ctcaccgagc gagcggaggc gcgagaaatt cgtgcccgtg cccgaaccgt
     5941 atttgaacag acgcgcctcg cgcacccaca gatccatgat ccccccttca ttcaccagat
     6001 cgtcgttgat cgactggatg aagcaggcgt gcggctgcgg gcgctcgtag gcgctcttgg
     6061 tctcggtcag cgtgtgggtc tcttcatcga catagtagtg cccctgggcc gggcccgtaa
     6121 tgttgtaggc ccagttgaga ccggtgttga accactgcgg cgaattcggc gccgcgatct
     6181 gcgcggccag catgtacgac agctcctcgg cgaacgcccg cgcatcggcc tcccccgaaa
     6241 agtaaccgta gcgctctccc cagtaccgcc agcagccggc caggcggtgg aacacctggc
     6301 gggaatcggt ctcgccgccc gtcttcggct gccccttctc gtcgagaacc ggccggccgt
     6361 cctcgtcccg gagcgggacg ccggctttgc ggaaatactt ctgggcgaga atatccacgg
     6421 ccacctgcga ccacgcctcc ggaacgtgga tatccttcag ctcgaagacc accgagccgt
     6481 cggggttgac gatccgcgag gatcgtttcg caaagggaat gcggctgtac ggcgactcac
     6541 cgtcccgcgt gaaataccgc tgtattttca aagcatacct cctgcagatc tctgctcgcg
     6601 gctcatcggg gcgcgtccat gcggtaatcg gagatcacat ccggatggcg tttgagtagc
     6661 agtctgcccc acttgctgtg tgtttcgctg tcatcgttac aggcgaatca ggtcgctgtc
     6721 tgtaattagg tggccacaag atataggggt cggcttttgt caagccacaa aaaatggtgt
     6781 ttttattggg taattcatat ttctttggcg ggcaagcggc tgcgcagcag aaccggttgc
     6841 cgggggagac acgcgccgtc cacgactttc catcgttgaa gaaatcgagt tcggtaagct
     6901 attgtttcat gggacagtac gcggacatcc gttcttaacg gcgccgcggg aaaccggcgt
     6961 tgaattttca acaacagcta atctcttgta ggtcctgctc ttacggggga cttcaccggt
     7021 tatcaacagg ataaccacag gatcgtcaca aaacgtgtgg acaaagccga tgtgccgtcg
     7081 ggagaaacat cgattttttg ccggattttc tgcagttttc ccttgacaag tcgccccccc
     7141 tgggttaatt tactggtgca atcggaaagt gttactcttt ctgtggatta cctaatatcg
     7201 tccacccagg caggattcgt cccctgcttg ggtttttttt tgagccgtgc aagtgcttcc
     7261 ccaagcgccc ccgcaatcct gctctcccaa cgatccccgt cttagtaacc gaaccgtcgc
     7321 cgatcagacg gtcgtccccc tcgaccgcag gcagatccgt cggcgcgccc cgagccgaaa
     7381 cccatttgac accccgcatc ccccggccta cctttgctgc aaatgaacat gcccaaccgc
     7441 ctgacgctcc ttcgggtcat catcgccccg atcttcatgt acttcttcct gctcgacagc
     7501 tactggatga aactcacggc gctcgtgttg ttcgtggtgg cggcgctgac cgatttcgcc
     7561 gatggctact acgcccggaa gtacggcatc attacgggtt tcgggaagtt catggatccg
     7621 ctggcggaca aggtgctcgt ttccagcgcg ctgattacgt ttatcgcgct cgacgtggtc
     7681 tcgccgctgc cggtgatcct catcgtgggg cgcgagttcc tcatcaccgg gctgcgcatg
     7741 ctggcggcct accggggggt ggtcatccct ccgacctggt gggcgaaggt gaagactttt
     7801 ctgcagctct cggtggtcgg gctggtgctg gcgtacatca acctgattct cacgctcgag
     7861 cactttgaca gtccggcgcg gccgtatttt cagtttgatt acccctttta ttttaatatc
     7921 ctcctgtgga ttacggccgc ggtgacggtc tggacgggaa tcgactatat caggaaatac
     7981 tactacatga tcaagacggt gctcaagtga ggggcggcga atgaagcgcg gcctggtcca
     8041 actcatcgcc ggcgggctgt acagcggcgc ctcgccgatt gtccccggca ccaccgggtc
     8101 gattcccccc tggctcatcg cctggtttct cctggcgggc aaccaggcgg cgctcgggat
     8161 tgtggccgtc ctgaccgtct tcgtctccgt ctgggccgcc ggggaggcgg agcgcttcta
     8221 cggccacgat gcgaagaaga ttgtgatcga cgaatgggcg ggcatgttcc tgaccctgct
     8281 gttcgtgccg tactcgctgg tgaactacct catcgggttt gcggcgttcc gggctttcga
     8341 cgtggtcaag atcccgccgg cggcgcaggc cgagcggctc ccgcgcgggt ggggagtgac
     8401 catggatgac gtcgtggccg gcgtgcaggc gaacatcctg acccagattg tggtccgcct
     8461 catcgcccgc tctgcgggcg cctgaggccg gaggatggcg gcgatgccca tgtgcgcgga
     8521 aatcatcacg gtcggcgacg agctcatcac cggccaccgg atcgacacca actcggcgtt
     8581 catcgcccag cagttgaccc ggatcgggct cgaggtccgg taccgaagct cggtcggcga
     8641 ttcgctcgaa atgatggagg aggtgttccg cctggccctc aaacgggccc aggtcgtgat
     8701 tgtcaccggc gggctcgggc cgaccgacga cgacatcacc aagaaggcca ttgtccgcgt
     8761 cttcaaacgc aacctcattt tccatgagga gctgctcgaa gagctgaaga agcgctacgc
     8821 ggcgcgcggt atcgagatgc ccgccatcaa ccagaaccag gcgctcctcc cgcagggggc
     8881 gaccgttttc ccgaacaagt acggctcggc ggtcggaatc ggaatcgccg agggcgggac
     8941 ggtgttcctg gccctgccgg gcgtcccgta cgaaatgaag cagatcatga ccgatgagat
     9001 catcccgtac ctccagcgcc tcaacatcgg gcaggtgctc gaggttgtga gtctgcggac
     9061 gacgggagcg gtcgagtcca agctcgcgga gttgatcgcc ccggggttga agctggagcc
     9121 gggggtgaaa ctcgcctacc tgcccgcccc gtcggccatc gaactgcgcg tcctggcggc
     9181 cgccgctacc cgcgaggagg cgcaggagaa ggcgcaggcg gtggtccggt atctcgaatc
     9241 gacggtgggg aagtacattt tcggccgcgg cgacgacacc ctcgagggag tggtgggaca
     9301 gctcctggtc gacaacgaca agtccctcgc cgtggcggaa tcctgcaccg gcggcgagct
     9361 cggcatgctc atcacctcgg tccccggcgc ctcgcgttac ttcctcggcg gcgtggtggc
     9421 ctactccaac gaggccaaga cggaactgct cggagtggac cgggacatca tcgcgcagtt
     9481 cggcgcggtg agccgggagt gcgcggaggc gatggccgag gggtgccgcc ggcgtttcgg
     9541 cgccgactac gccctggcgg tcacaggcat cgccgggccc gggggcgcca cgccggacaa
     9601 acccgtgggg accgtctgga tcggcctcgc ctccctccac gaaacctacg cgaaaacgtt
     9661 cagcctgggc acgctgcggg agagcatccg cgaccgggcg gcctacacgg cgctggagat
     9721 gctgcggcgc aacatcctgg atattacctg atgcggttgt ttatcgcttt gccgccggcg
     9781 gaggcggtcg cggaggaact cggccgcgtg atcgtgctcc tgcgcgcaaa aggggggccg
     9841 gtccgctggg tggcgccgga gaacatccat ttaaccgtgc ggtttctcgg cgagacggac
     9901 gaggcgcggg tgccgaagct gcggagtctg atcgacagca tcgcgccggc cttcgccccg
     9961 gccgacgtcc tgatcgaccg tctcggcggt tttcccaatc tccgcaagcc gcgcgtgatc
    10021 tgggccggac tggccgccgc cgagccggtc gagcggatgg cgcagatggc gcggcaggtg
    10081 gagctcgcag tgcggcaact gcgtttcgag ccggaaaaga aaggcttcca gccgcacctg
    10141 acgctcggcc gggtcaaaga cccgcgcggg atggagcaac tggcggcctt catggaatcg
    10201 taccgcttca cgccgctcgc cgtgcgcctc gaccgtctcg tgctgttccg gtcgacgctg
    10261 actccgaaag ggtcgatcta cgagcgcgtg catgaggcga agctgggcga cgagcggctg
    10321 gggggctgag caccgtgcgc cggcccattc aagtcctcat tttcgtctat cgccagaccg
    10381 cccggggacg ggaatatctc atgctccgcc gcattcccga gctccgcggg ttctggcagc
    10441 cgatttcggg cggagtggag gacgatgagg atctcctgac cgcggcccgc cgcgagttgc
    10501 gcgaagagac cgggtttcag acggccgccg tcgaatcaat caactacgcc taccgcatcc
    10561 cggccttcca cgaccgcaag ctcggcgccg ctccgggcgc cgatgagacc atgccgctgc
    10621 acaccttcgc ggcccgggtc gacgatgggg cggagccggt cctcgatccc gccgagcacg
    10681 accgctacct gtggtgcccg ctccttctgg ctgatcggct gctctactgg ccgggggacc
    10741 gcgaagcgct gcgccaggtg gacacctggc ttcggtcccg gccgctctag gccggcttca
    10801 gcacccgccg cgcgcagaca cgggaccggc ggcgcccacc tcacgatgag cacgggtacg
    10861 gcgcggaccc gccgcggaac aggtgggcaa tcaggtaagt cacatcggcg accgccacga
    10921 tcccggacag atcgacatcg cccgcttcgt aacacgccgg ctcgggcccc ccgcggaaca
    10981 gatacgagat caggaaggtg agatcggtga cgttgaggga gccgctgttg tcgacgtcgc
    11041 cgcgggtccc gacgcagcaa ttctggtaga cgaggaccgg cgagacggtc ttgggcgcgt
    11101 aggcggcgat gctgctgacg aagcgggggg tgaacgagcc gtagccgttg aacgagacga
    11161 ccgccgagtc gcccgaggcc gccccggccg tggtgaacca gagtctcact accgggccgc
    11221 tgccgggcgg aagcggcgag gaaccggagg gggcgctcac gagctgaacg gttccctgct
    11281 tgagcgaatt gttgaggtcg atgaacccgc ggacggcgaa gtagtcggtc cggcagcccg
    11341 aggtggtcag cgagtccaga gtcaggtcga gcgtccccgc gaaagtaacg gggatgcgga
    11401 tctcggagag gggaaggtta ttcgcggcaa agaccgccaa ttcgacctcg gagaaccgcg
    11461 ggaccgaccg gtcatccgcg cggagcgtat cggcgaggac ggcgatgtag tcggcgcgtt
    11521 cgcggcgcgc gctctcggcg cccgccgtca cctgcagcga cacgtcgtac agcccgggcg
    11581 ccgcgtacac gtggccgggc gactggacga aggccgagtc cccgtcgccg aacgaccagg
    11641 cccacgtgtc gacatccaaa agcgacgtcc cggtgaactg caccgccagc ggcgcatcgc
    11701 cgagctgcgg atcggccgtg aaagcgacgc cggccgggtc a
//
LOCUS       DAOPOE010000094        11617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6401_length_11617_cov_4.928559, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000094 DAOPOE010000000
VERSION     DAOPOE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_11617_cov_4.928559"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKM94_11165"
     CDS             complement(<1..841)
                     /locus_tag="PKM94_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD67234.1"
                     /translation="MDEPILIYEKSKPGRTGYTLPPTASREADLLAALPAEFRRSKEA
                     ALPEVTEGEAVRHFVGLSVKNHHIDKGFYPLGSCTMKYNPKLNDAAAALPDFRDHHPL
                     APCSSAPGILRLMWDLQQFLSEVSGFPSISLQPVAGAHGEFTGLLIMRAYHLSRGNKR
                     SKVIIPDSAHGTNPASVAAVGYSTVQVKGNERGVIPPEAVAAAMDEDVAGIMMTNPNT
                     LGIFETHIREIAAIVHAKGGLVYMDGANLNANMGIFRPGDVGFDIMHFNLHKTFSTPH
                     GGGG"
     gene            complement(883..1551)
                     /locus_tag="PKM94_11170"
     CDS             complement(883..1551)
                     /locus_tag="PKM94_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67235.1"
                     /translation="MLDTVVFDDPKAIAFFTNEMLLVKLNAEVDTLTKQRYNIMGYPT
                     SVLVDKEGNEIDRFVGFGETDEYIGTFRNYARGIGTLDDMAARFEVNQNRDSAFAIAE
                     KYKYRGAVAEADQWYDRVVAMGAPTDSLAGEARVAQADAHLRAKDFAPALAMFEQIRA
                     DFAGTRFAEDAEIYRAIAFGRQNDTAQAIAAFSEFLDHWPESEAADYAKEQLRKLKGE
                     EPSE"
     gene            complement(1564..1734)
                     /locus_tag="PKM94_11175"
     CDS             complement(1564..1734)
                     /locus_tag="PKM94_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67236.1"
                     /translation="MQKWAVIAALALAAVLVQCAKKEQAAVQAPFVENLADALQKAQA
                     GQYIVVDFYTDW"
     gene            complement(1752..2153)
                     /locus_tag="PKM94_11180"
     CDS             complement(1752..2153)
                     /locus_tag="PKM94_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD67237.1"
                     /translation="MGYCPNCRWEYELDVLVCPACGAALAEARPGTGGAAERPDRSWV
                     AMGRVLTSGQSKIAKASLDAGNIPSVILPTPLYGAGRSAAPAAGLRRAGDEGQLIMVP
                     REFERDAQMALAGVLGSAAVEFGRFEEREDN"
     gene            complement(2153..2428)
                     /locus_tag="PKM94_11185"
     CDS             complement(2153..2428)
                     /locus_tag="PKM94_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67238.1"
                     /translation="MKQEAEQGWVVVGTVDDKPFADMARETLQACEVPAVVISRSGFF
                     GNVGLPLNPIYETKPASFEISVPAEFRDEAIDVLDATLGDKWRRKED"
     gene            complement(2478..3833)
                     /gene="gcvPA"
                     /locus_tag="PKM94_11190"
     CDS             complement(2478..3833)
                     /gene="gcvPA"
                     /locus_tag="PKM94_11190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOD67239.1"
                     /translation="MPYVPNTDDDRRDMLKRIGVDKFEDLLEGIPSELRLKRELNIPA
                     LSEMELLREIEALSRESREGLAGFAGGGVYDHFIPAAVGAITSRPEFMTAYTPYQAEV
                     AQGTLQAIYEYQTHVCRLTGMDVANASMYDGATAAAEAAMLAVKATGRNRMVAAETLN
                     PMYREVIRTYLSGLDVEIVTAPMADGVTDLNRLEDAIDEHTAGVILAQPNFFGRLEEI
                     GPVEQMIHRVGGKLVLAFDPIAQAVLKTPGEWGADIAVGEGQPLGLPVSFGGPLLGMF
                     AVKKDLVRSMPGRLAGRTTDVEGKPGFVLVLQTREQHIRREKATSNICTNEALCATTA
                     AVYLTLLGRTGLKRVALLSMERAQQAARALTAIDGYELYWPKPFVREFAIRTPKPAKQ
                     IILAMIERGVLPGVAAGRWYQGLDHCLIVALTEKRTEAEIGRLADGLKELAASGVLSR
                     M"
     gene            complement(3963..4346)
                     /gene="gcvH"
                     /locus_tag="PKM94_11195"
     CDS             complement(3963..4346)
                     /gene="gcvH"
                     /locus_tag="PKM94_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOD67240.1"
                     /translation="MNIPAELKYTKEHEWIRREGQTATIGITDYAQGELGDIVFVELP
                     RVGSVVTQMKAFGTIEAVKAVSEMFAPVSGRVTAVNGALEDDPMVINRDPYGEGWMIK
                     VEVSDPGQLDALLDAAGYQKLLAAV"
     gene            complement(4373..5881)
                     /gene="accC"
                     /locus_tag="PKM94_11200"
     CDS             complement(4373..5881)
                     /gene="accC"
                     /locus_tag="PKM94_11200"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOD67241.1"
                     /translation="MFEKVLIANRGEIALRVIRACREMGLKTVAVYSEADRDALHVRF
                     ADEDVCIGPPPARQSYLDFKHIIAAAEVTNAGAIHPGYGFLAENADFAEICESCGLAF
                     IGPTPEQIRQLGDKVQAKDLMRKAGVPCIPGSEGAVATFREAATVAEEVGYPVILKAV
                     AGGGGKGMRICRDVAELERGFHVASAEAGNAFGNPDLYLEKVVLNPHHVEIQVIGDTY
                     GNYLHFGERDCSIQRRHQKLIEETPSPLMTPELRRRMGEAALNGAAQVGYRGVGTMEF
                     LVDDQRNFYFMEMNTRIQVEHPVTEEAYEVDLLQDQIRVALGERLPYTQEEIKPRWAV
                     IECRINAEDPEKDFRPTPGTITGLHIPGGPGVRVDRAVYTGYQIPPHYDSMIAKLIVR
                     GRTRQEAVVRMRRSLEEFIVEGVPTTVAFHREIFEHPDFVAGRYDINFLEQRFRKGQE
                     APPAPEEAQEEAIAAGETVSRLTLLRGSEAGVLAEEETVETGAPDSASDNKQ"
     gene            complement(6177..7346)
                     /locus_tag="PKM94_11205"
     CDS             complement(6177..7346)
                     /locus_tag="PKM94_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOD67242.1"
                     /translation="MNLKQMLVEMLNRKASDLHIRVGVRPHLRVNGKLDEISTDPLTI
                     DIMEQIVGQILNEKQRERFYRKNEMDLALSVAKLGRFRINLFRQRGTTAIAIRAVNTT
                     VPTFEELHLPPVIRELAKTRRGLIIITGTTGSGKSTTLATMIEEINGTRHDNILTVED
                     PIEYIYRDKKSIISQREVGGDTETFASALRHAFRQDPDVILIGEVRDLETMSIALTAA
                     DTGHLVLTTLHTLNVVETISRIISFFPPHQHQQIRLLLGGTLKAIVCQRLLVRSDMPG
                     RVPAVEVMINSGAVREAIMDPEKTVNIPELMEQGTVQYGMQTFDQSIMKLYKTGKITF
                     EEAIQQASNPDDFDLRVKGITGTSDRWDQNENESGDAEKPSRQHSAEASGGFLKY"
     gene            complement(7377..7880)
                     /gene="accB"
                     /locus_tag="PKM94_11210"
     CDS             complement(7377..7880)
                     /gene="accB"
                     /locus_tag="PKM94_11210"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOD67243.1"
                     /translation="MNEKYIRKLIRLVEESDVASLEVSSWGRRVKIIHRAEASSNGHQ
                     QTALVATAPAPPAAPITPSAPAASEPVSTAPAAAPAQVSAEESRYVSIKSPMVGTFYA
                     APAPDAEPYVTINQKIRIGQVVCIVEAMKLMNEIESEVSGRIAKILVQNAEPVEFGQT
                     LFLIEPD"
     gene            complement(8109..9185)
                     /locus_tag="PKM94_11215"
     CDS             complement(8109..9185)
                     /locus_tag="PKM94_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOD67244.1"
                     /translation="MSAKRINALRKKLRESNLDGLIVTHLDFIRYLTGFSGSAGLLVI
                     GSNGAEFLTDFRYKDQSRKEVAGARVHIARGGDLLEGLKDLKGLADRNTRYGIDAAHL
                     TLAQHTRLRKVWPGVLLANAEDVLADLGWVKEPEELAAIRKAAAVSDTAFERVLSLVA
                     PGIRERELAAELEYQMMMLGSSKPAFETIVASGWRAALPHGAASDKKIEKGDFITFDF
                     GATIDGYVSDITRTVVVGKASPRQRKIYDIVLRAQLAGIRKVRAGVAGKAVDNVCRGI
                     IKRAGYDKHFGHGTGHGIGYYVHVGPRLSPLSNDILQPNNVVTVEPGIYISGWGGVRI
                     EDDVVVTRGGGKVLNRAPKNLLEL"
     gene            complement(9272..9859)
                     /locus_tag="PKM94_11220"
     CDS             complement(9272..9859)
                     /locus_tag="PKM94_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD67245.1"
                     /translation="MTDRRKVYLALGDSMSIDEYTGVIGGGAVRQFHECLGQDWILLD
                     RTCDGCTIAEVPTGEAADLITLTIGGNDALAVADIRATEADIAKVVRAHRALLLEIRK
                     SNPLSLLIVGNIYRPDSPLPENLLHLLRRLNRGIEQNVRECGGALADISGAFDGHESE
                     YLCRVIEPNLKGAAVIAGLFRQAYESWAEARDGSQ"
     gene            complement(9852..10619)
                     /locus_tag="PKM94_11225"
     CDS             complement(9852..10619)
                     /locus_tag="PKM94_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67246.1"
                     /translation="MFDLNPKEAQSSVERPAAGAYWPAPAAAALAAGKYSRAVELCRE
                     GLAEEPGAVAGRLIYARALYHAGQVESAAEQFYEVLARDPENAAALKYLGDIKWAEGD
                     VHGAVAHWRRVLEIDPYCRELRCDWRARERAADEPGSPASGEDQTASPSNAGAGEQLR
                     TAAAGNGPAGEGRATGTGAPLRTITLKRDGEQAAAPAERARLREVPFYTETVGDLYLA
                     QGHYRLAAEVYGVLYGRSAVPRLKEKLEQAERKVRSD"
     gene            10860..>11617
                     /locus_tag="PKM94_11230"
     CDS             10860..>11617
                     /locus_tag="PKM94_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67247.1"
                     /translation="MSKWVSTLLGLLLVASSAVAGGGEVWIATFDGTMGLQDQARGIA
                     VDPSGCVFVVGETNYASEPVEIPSSLLLIKFSPDGDTLWTHVNDSASSARYLVVDDSG
                     CVYVAGESKSDTVDFLVLKYGPEGDSIWAATYKHPVFTSDEIRGIVLDPNRNLYLLGD
                     VEDLADDEDIALVKILPDGSIDWVSILSGPAGDDQPEGIAVDNSGHIAVVGSSRNSDS
                     ATTAIWTIMYDASGDTVWTARYGGSTTCYVLSVGT"
BASE COUNT         1838 a         3997 c         3479 g         2303 t
ORIGIN      
        1 gcccgccgcc gccgtgcggc gtcgagaacg tcttgtgcag gttgaagtgc atgatgtcga
       61 agccgacgtc gccggggcgg aagatgccca tgttggcgtt aaggttcgcc ccgtccatgt
      121 acacgagtcc gcccttggcg tgcacgatcg cggcgatctc ccggatgtgg gtctcgaaaa
      181 tgccgagcgt gttgggattg gtcatcatga tcccggccac gtcctcgtcc atcgccgccg
      241 ccaccgcctc gggcggaatg accccccttt cgttcccctt cacctgcacc gtggagtagc
      301 cgacggccgc gaccgaggcg gggttggtgc cgtgagccga atccgggatg atgaccttcg
      361 agcgcttgtt ccctctgctc aggtggtagg cgcgcatgat gagcagcccg gtgaactccc
      421 cgtgcgcccc ggccaccggc tggagcgata tcgagggaaa ccccgacacc tccgacagga
      481 actgctggag atcccacatc agccggagga tgcccggcgc cgagctgcac ggcgcgagcg
      541 ggtggtggtc gcggaaatcc ggcagcgccg ccgccgcatc gttcagtttg ggattgtact
      601 tcatcgtgca cgaccccagc ggatagaacc ccttgtcgat gtgatggttt ttcaccgaca
      661 acccgacaaa gtgccgcacc gcctccccct ccgtcacctc cggcagggcc gcctctttcg
      721 acctgcggaa ctccgccggc agggccgcga gcaggtcggc ttcgcgcgac gccgtcggcg
      781 gcagcgtata cccggtccgc ccgggctttg acttttcgta tatgagaatt ggctcgtcca
      841 taattgtccc gtgcgacggg gctcttgcgg gcccggtcgc tgttattccg atggctcctc
      901 gcctttaagt ttcctcaact gctctttcgc gtagtccgcg gcttccgact ccggccagtg
      961 gtccaggaac tcgctgaagg cggcaatcgc ctgggcggtg tcgttctgcc gtccgaaagc
     1021 gatcgcccgg taaatctccg cgtcctcggc gaaccgcgtc cccgcaaaat cggcgcggat
     1081 ctgctcgaac atggccagcg ccggcgcgaa gtctttcgcc cgaaggtggg cgtctgcctg
     1141 cgccacccgc gcctcgcccg ccagcgaatc cgtcggcgcc cccatcgcca ccacccggtc
     1201 gtaccactgg tcggcctccg ccaccgcccc gcggtacttg tacttctcgg caatcgcgaa
     1261 cgccgaatcc cggttctggt tgacctcgaa ccgggccgcc atgtcgtcca gcgtcccgat
     1321 tccccgggcg tagttccgga aggtcccgat gtactcgtcg gtctcgccga atccgacgaa
     1381 gcggtcgatt tcgttgccct ccttgtcgac cagcaccgag gtcggatagc ccatgatgtt
     1441 gtagcgctgt ttggtcaggg tatcgacctc ggcgttcagc ttgaccagca gcatttcgtt
     1501 ggtgaagaac gcaatggcct tgggatcatc gaacacgacc gtgtccagca tcttgcagta
     1561 ctttcaccag tcggtgtaga agtcgacgac gatgtactgg cccgcctgcg ctttctgcaa
     1621 ggcgtcggcg aggttctcca cgaacggcgc ctgcactgcc gcctgttcct tcttcgcgca
     1681 ctgcaccagc accgccgcca gcgcgagcgc cgcgatcact gcccattttt gcatgctgcc
     1741 gtacctccta actagttgtc ctcgcgttcc tcgaatcgcc cgaactcgac ggcggcgctc
     1801 cccagcacgc cggcgagcgc catctgcgca tcccgctcga actcgcgcgg gaccatgatc
     1861 aactgccctt cgtcgccggc ccgccgcaat cccgcggccg gcgcggcgct ccgcccggct
     1921 ccgtagaggg gggtcgggag aatgaccgac gggatattgc cggcgtcgag cgacgccttg
     1981 gcgatcttcg actgcccgct cgtgagcacc cgccccatcg ccacccagct gcggtccggc
     2041 cgctcggccg cgccgcccgt tcccggacgc gcctccgcca gagcggcccc gcacgcgggg
     2101 cacaccagga cgtcaagctc gtattcccac cgacagttcg ggcagtatcc cactaatctt
     2161 cctttcgccg ccacttgtcc ccgagagtcg cgtcgagcac gtcgatcgcc tcgtcgcgaa
     2221 actcggccgg cacggatatt tcgaacgacg ccggtttggt ctcatagatc ggattgaggg
     2281 ggagtccgac gttgccgaag aagccggacc gggagatcac gacggccggg acttcgcacg
     2341 cctggagcgt ttcgcgcgcc atgtccgcga acggcttgtc gtccaccgtc cccaccacca
     2401 cccaaccctg ttccgcttcc tgcttcactt tgctcccaca gatgtaacac cgttccggat
     2461 tcccttgcag ggccatatta cattcgggac aatacgccac tcgccgccaa ctcctttagg
     2521 ccgtcggcca gccgtccaat ctccgcctcg gtccgttttt cggtgagggc gacgatcaag
     2581 cagtggtcga gcccctggta ccagcgcccg gccgcgacgc ccgggaggac tccccgttcg
     2641 atcattgcca ggatgatctg cttggccggc ttgggcgtcc ggatggcgaa ctcgcgcacg
     2701 aagggtttcg gccagtacag ttcgtatccg tcgatcgccg tcagcgcccg ggccgcctgc
     2761 tgcgcccgct ccatcgacaa tagcgccacc cgcttcaggc cggtcctgcc cagcagggtc
     2821 aggtaaaccg ccgcggtggt cgcacagaga gcttcgttgg tgcagatatt cgaggtggcc
     2881 ttctcgcggc ggatatgctg ttcgcgcgtc tgcagcacca ggacaaagcc cggcttccct
     2941 tcgacatccg tcgtccgccc ggccagccgc cccggcatgc tgcgcaccag atctttcttg
     3001 accgcaaaca tccccagaag cggcccgccg aacgacaccg gcagtccgag gggctgcccc
     3061 tcgcccaccg cgatgtcggc gccccactct cccggcgtct tgagcacggc ctgggcgatc
     3121 gggtcgaacg ccagcaccag cttgccgccc acgcggtgga tcatctgttc gaccgggccg
     3181 atctcctcga gccggccgaa gaaattcggc tgggcaagaa tcacgccggc cgtgtgctca
     3241 tcgatcgcgt cctccagccg attcaggtcg gtcacgccgt cggccatcgg cgctgtcacg
     3301 atctccacgt cgagaccgga gaggtaggtc cggatcacct cccgatacat cgggttgagc
     3361 gtctcggccg ccaccatgcg gttgcgcccg gtcgctttga ccgccagcat ggcggcctcg
     3421 gccgccgccg tcgccccgtc gtacatcgag gcattcgcca cgtccatccc ggtcagacgg
     3481 cagacgtggg tctggtattc gtagatcgcc tggagcgttc cctgcgccac ctccgcctgg
     3541 tagggagtgt aggcggtcat gaactccggg cggctggtga tcgcgcccac ggccgcgggg
     3601 atgaaatggt catagacgcc gcccccggca aagcccgcca gaccctcgcg gctctcgcgg
     3661 gagagcgctt cgatttcccg gagaagctcc atctccgaga gcgccgggat gttgagttcg
     3721 cgcttgaggc gcagctccga gggaatcccc tccagcaggt cctcgaactt gtccacgccg
     3781 atgcgcttga gcatgtcgcg gcgatcatcg tcggtattcg gtacgtacgg catcgctgtt
     3841 tacttccgat cctttgtgtt gtttcgcccg attccggggg tcgctggtgc gctttcgggg
     3901 cggccctccc ggcgcgccgg cccggagttt acccgggggc cgggccggtg cggcgcctgg
     3961 gcctagaccg ccgccagcag tttctgataa cccgcggcgt cgagcagggc gtccagctgg
     4021 ccggggtcgg acacctcaac cttgatcatc cagccctcgc cgtaggggtc gcggttgatc
     4081 accatcggat cgtcctcgag cgcgccgttg accgccgtca cgcgtcccga aacgggcgcg
     4141 aacatttccg agaccgcctt cacggcctcg atcgtgccga acgccttcat ctgtgtcacc
     4201 acactgccga ctctgggcag ttcgacgaag acgatatcgc ccagttcccc ctgggcgtag
     4261 tcggtaatcc cgatggtcgc cgtttgtcct tcgcggcgaa tccactcgtg ctctttcgta
     4321 tacttgagtt ccgcgggtat gttcaccgga tcctccaatg agcctcacgt ccttactgct
     4381 tgttatccga tgccgagtcg ggcgcgcccg tctcgaccgt ttcttcctcc gccagcactc
     4441 cggcctccga cccccgcaag agcgtcagcc ggctcactgt ttcccccgcc gcgatcgcct
     4501 cctcctgcgc ctcctccggc gcgggcgggg cctcttgtcc cttgcggaag cgctgctcga
     4561 ggaaattgat gtcgtagcgg ccggcgacga agtcgggatg ttcgaaaatc tcccgatgga
     4621 acgccaccgt ggtcggcacg ccctcgacga tgaattcttc cagcgagcgg cgcatccgga
     4681 ccacggcctc ctggcgcgtc cgccccctga cgatcagttt cgcaatcatg gaatcgtagt
     4741 ggggcgggat ctggtagccg gtgtacacgg cccggtccac ccgcacgccc ggtcctccgg
     4801 gaatgtgcag cccggtgatc gtccccggcg tcggccggaa atccttttcg gggtcctcgg
     4861 cgttgatgcg gcactcgatg accgcccagc ggggcttgat ctcctcctgg gtgtacggaa
     4921 gccgctcgcc cagcgccacc cggatctggt cctgcaggag gtcgacttcg taggcctctt
     4981 cggtcaccgg atgttccacc tggatccgcg tgttcatttc catgaagtag aaattgcgct
     5041 gatcgtcgac gaggaactcc atcgtcccca ccccgcggta tccgacctgg gcggcgccgt
     5101 tgagcgccgc ttcgcccatc cgccgccgca gctcgggcgt catgagcggt gagggcgtct
     5161 cctcgatcag cttctggtgc cgccgttgaa tcgagcagtc acgctcgccg aaatgcaggt
     5221 agttgccgta ggtgtcgccg atcacctgga tctcgacgtg gtggggattg agcacgactt
     5281 tctccaggta gagatcgggg ttgccgaagg cgttcccggc ctcggccgag gcgacatgga
     5341 agccgcgctc caattccgcc acatcgcggc aaatccgcat ccccttgccg ccgccgccgg
     5401 cgaccgcttt caggatgacg gggtagccga cttcttcggc caccgtggcg gcttcccgga
     5461 acgtcgccac cgcaccctcc gatcccggaa tgcagggcac gccggccttt ctcatcaggt
     5521 ctttggcctg gaccttgtcg cccagttgcc ggatctgctc cggcgtcggg ccgatgaacg
     5581 ccagcccgca tgactcgcaa atctccgcga aatcggcgtt ttccgccaaa aaaccatagc
     5641 cggggtggat ggcgccggcg ttggtcacct ccgccgccgc aatgatatgc ttgaaatcga
     5701 gatacgactg ccgggccggg ggcggcccga tacatacatc ctcgtcggcg aagcgcacgt
     5761 ggagggcatc gcggtcggct tcgctgtaca ccgcgaccgt cttcagcccc atctcacggc
     5821 acgcccggat cacccggaga gcgatttcgc ctcgattggc aatgagtact ttctcgaaca
     5881 agcacagtcc tttcgtcttt tcgttgacag cccggacagg gtgctgcccg gaccgcacgt
     5941 cacagatagg tattataccc tattcgtccc ccgaattcaa ctcaataaac gctcccgcgc
     6001 gtccggtgtt cccccctttc tgggacgata agatattgtc gcacaatcac taacaaagag
     6061 gcgcagagga tgcgcataag gaaaagagac acccccatcg gggtgtctct ggataagcgg
     6121 tgctttgtcc ggatcggcgc ggacccggcg ggggcgcggc cttcgcgcct ccccgctcag
     6181 tacttgagga atccgcccga cgcctcggcg gagtgctggc gggacggttt ctcggcgtcc
     6241 ccggactcgt tctcgttctg gtcccagcgg tccgaggtcc cggtaatgcc cttgacccgc
     6301 aggtcgaaat cgtccgggtt cgaggcctgc tgtatggctt cctcgaaggt gatcttcccc
     6361 gtcttgtaca gcttcatgat cgactggtcg aacgtctgca tgccgtactg caccgtcccc
     6421 tgttccatca gttccggaat gttcacggtt ttttccggat ccatgatggc ttcgcggacg
     6481 gcgccggagt tgatcatcac ttccactgcc ggcacccgcc cgggcatatc cgagcgcacg
     6541 aggagccgct ggcacacgat cgccttgagc gtgcccccca gcagcagccg gatctgctgg
     6601 tgctggtggg gcgggaagaa cgagatgata cgcgagattg tctcgaccac gttcagcgtg
     6661 tgcagcgtgg tgagcacaag gtgccccgtg tccgccgccg tgagcgcgat cgacatcgtc
     6721 tccagatcgc gcacctcgcc gatcaggatc acgtcgggat cctggcggaa agcgtggcgc
     6781 agggccgagg cgaaggtctc cgtgtcgccg ccgacctcgc gctgcgagat gatcgacttc
     6841 ttgtcccggt agatgtactc gatcgggtct tcgacagtca ggatgttatc gtggcgggtc
     6901 ccgttgattt cctcgatcat ggtcgccagg gtggtcgatt tacccgaccc ggtcgtgccc
     6961 gtgatgatga tcagtccgcg ccgggttttc gccagctcgc ggatcacggg cgggagatgc
     7021 agttcttcga atgtcggcac tgtagtgttg accgcacgga tggcgatcgc ggtggttccc
     7081 cgctgacgga acaggttgat acggaagcgg ccgagtttcg ccaccgacag cgccaggtcc
     7141 atttcatttt tgcggtagaa ccgctcgcgc tgcttctcgt tgagaatctg gccgacaatc
     7201 tgctccatga tgtcgattgt caacggatcg gtggagatct cgtccagttt gccgttcacc
     7261 cgaaggtggg ggcgcacccc gacccgaata tgcaggtcgg acgctttccg gttgagcatc
     7321 tcaaccagca tctgcttgag attcatgggg aaaccccttc tgctgttcgc ctgttctcag
     7381 tcgggttcga tcaagaacag agtctgcccg aattccacgg gctcggcgtt ctgcaccagt
     7441 atcttggcga tccggccgct gacctcgctc tcgatctcat tcatcaattt catcgcctcc
     7501 acgatgcaca cgacctggcc gatgcgaatc ttctggttga tcgtcacgta cggttcggca
     7561 tcgggggccg gggccgcata gaaggtcccg accattggcg actttattga tacatatcgg
     7621 ctttcctcag ccgatacttg agccggagca gccgccggag cagtggatac gggctcggaa
     7681 gccgctggcg cggaaggagt tattggcgcc gcagggggtg ccggagccgt cgccaccagc
     7741 gcggtttgct gatggccgtt cgatgatgcc tccgcccgat ggatgatctt cactcgtcga
     7801 ccccaactgg atacctccag cgaggccaca tccgactcct ccaccaggcg aatgagcttt
     7861 cgtatgtatt tttcgttcat aagaaacatc ttttcctgat ccgcccttct ctcacgttgc
     7921 cgccttggca agggcgccgc ccgtactctc ccttttaccc gcgggcctca cgcaaaggcg
     7981 ttcttcccgt acatccgggc catgcggtcc cggcttggcc tgtaccgagc tctccctccg
     8041 ccctaccggt cgtgtccggc cggtcgctgc cggcgcaggc atccggcgaa atgataacca
     8101 gagcgcagct ataactccaa caagtttttt ggcgcccggt tcaacacttt cccgcccccc
     8161 cgggtcacca ccacgtcgtc ttcgatccgc actccccccc accccgatat gtagatcccc
     8221 ggctccaccg tcaccacatt gttgggctga aggatgtcgt tcgacagcgg cgacagccgc
     8281 ggcccgacgt ggacgtagta cccgatcccg tggccggttc cgtgcccgaa atgcttgtcg
     8341 taacccgccc gcttgatgat gccccggcag acgttgtcca ccgccttgcc cgccacgccg
     8401 gctcggacct tcctgatccc cgccagctgc gcccggagca cgatgtcgta gattttccgc
     8461 tgccgcggcg aggctttccc caccacgacc gtccgcgtga tgtccgagac atacccgtcg
     8521 atcgtggccc cgaaatcgaa cgtgatgaag tcccccttct cgattttctt gtcggaggcg
     8581 gccccgtggg ggagggcggc ccgccatccg gaggcaacga tggtttcgaa cgccggtttt
     8641 gaggatccca gcatcatcat ctggtactcg agttcggccg ccagttcccg ttcgcggatt
     8701 cccggcgcca ccaggctcag cacccgctca aacgccgtgt ccgacacggc cgccgctttc
     8761 cggatcgccg ccagctcttc cggctccttg acccacccga ggtcggccag gacatcctcg
     8821 gcgttcgcca gcaacacgcc cggccagact ttccggagcc gcgtgtgctg ggccagggtc
     8881 aggtgcgccg catcgattcc gtaccgcgtg ttgcggtcgg caagcccctt gaggtctttc
     8941 agaccctcca gaaggtctcc cccccgcgcg atatgcaccc tggcccccgc gacctctttc
     9001 ctggactgat ccttgtaccg gaagtcggtg agaaactccg ccccgttcga cccgatcaca
     9061 agcagcccgg ccgatcccga aaacccggtc aggtagcgga tgaaatccag atgcgtgaca
     9121 ataagcccgt cgagattgct ctcccggagc ttcttccgca gcgcgttgat ccgttttgcc
     9181 gacatgagat atttctcctc catcgaggtg agctttttgt tcgcttgccc cggcgacgcg
     9241 ggtcgccggc cgccggatat gggagagcgc ctcactgtga cccgtccctg gcttccgccc
     9301 aggactcgta ggcttgccgg aagagccccg cgatcaccgc ggctcctttg agattgggtt
     9361 caatcacccg gcagaggtac tcgctctcat ggccgtcgaa agcgcccgag atatcggcca
     9421 gagcgccccc gcattcccgc acgttctgct caattccgcg gttgagccgc cgcaggagat
     9481 gcagcagatt ctcgggcagt ggggaatcgg gtctgtagat gttgccgaca atcagcaggg
     9541 ataggggatt cgacttcctg atctcaagga gaagcgcccg gtgcgcccgc acgactttcg
     9601 cgatgtccgc ctcggtcgcc cggatatccg ccaccgccag ggcgtcgttg cccccaatgg
     9661 tgagcgtgat aagatccgcc gcctccccgg tcggaacctc cgcaatcgtg cagccgtcgc
     9721 aggtgcgatc aaggagaatc caatcctgac ccaggcactc gtgaaactgg cgcacggcgc
     9781 cgccgccgat gacgccggtg tactcatcga tactcatgct gtcccccaga gccaggtaga
     9841 ccttccgtcg gtcagtcact gcgcaccttc ctctccgctt gctccagctt ttccttcagg
     9901 cgggggaccg ccgagcggcc gtagagcacg ccatagacct cggctgcgag ccggtagtgt
     9961 ccctgggcga ggtacaaatc cccgactgtc tcggtgtaga aaggcacttc gcgcaggcgc
    10021 gcccgctccg ccggcgcggc cgcctgttcc ccgtcccgtt tgagggtaat ggtccgcagc
    10081 ggcgctcccg tgcccgtcgc gcgtccctcg ccggccggtc cgttccccgc cgcggccgtc
    10141 ctgagctgtt cccctgcccc ggcgttcgac ggtgacgcgg tctggtcctc tccggaggcg
    10201 ggcgacccgg gttcatccgc cgccctctct ctcgcgcgcc agtcgcaccg cagctcgcgg
    10261 cagtaggggt cgatttccag cactcgccgc cagtgggcca ccgcgccgtg cacgtcccct
    10321 tccgcccact tgatgtcgcc gagatatttc agcgccgcgg cgttttcggg atctcgcgcc
    10381 agcacctcat agaactgctc ggcggccgat tccacctgcc cggcgtggta caacgcccgc
    10441 gcgtaaatca gccgccccgc gaccgctccc ggctcttcgg ccagtccctc gcggcagagt
    10501 tcgaccgccc gcgagtattt tcccgcggcg agcgcggccg ccgccggcgc cggccagtag
    10561 gcacccgccg ccggccgctc gaccgacgac tgcgcttcct tcgggttcag gtcaaacata
    10621 aaagtgaagg taccgcaaaa ggcgcggccg cgcaagcgcc gggcggggcg aagtcgcccg
    10681 ttctctctct tcggcggctc cccggccgga tccggcgctt ccgcttgact ttgctgccgc
    10741 ggtgaccgta cttgccaatg tggagcggtt tgcccacacg cgggcgtgaa ttccagcgca
    10801 gggttaggcc acagactgat gcttggccac cctcgataca acaaggagga ggttttttca
    10861 tgagtaagtg ggtttccact ctcttgggtt tgctgctggt tgcgtcctcg gcggtcgccg
    10921 gcggaggcga ggtgtggatt gcgacgttcg acgggactat ggggctccaa gatcaggccc
    10981 gcggcatcgc ggtggatcct tccggatgcg tgtttgtcgt cggagaaacc aattacgcct
    11041 cggaaccggt cgaaatccca tcaagcctgc tgctcatcaa gttctcgccg gatggcgaca
    11101 ccctgtggac gcacgtgaac gacagcgcat ccagtgcccg gtatctcgtc gtcgacgaca
    11161 gtggctgtgt ctatgtagcc ggcgagtcaa agtcggatac cgtcgatttc ctggtcctca
    11221 agtacggccc cgagggcgac tcgatttggg cggcgaccta caagcaccct gtctttacga
    11281 gcgatgagat ccgcggcatt gtgctcgacc cgaaccgcaa cctgtacctg ttgggcgatg
    11341 tcgaggattt ggcagacgat gaggacattg cccttgtgaa aattctgccg gatggttcca
    11401 tcgactgggt ttccatcctc tccggtcctg ccggcgacga tcaacccgag ggaatagctg
    11461 tcgacaattc tggtcacatc gctgtcgtcg gctcgagcag gaattccgat agcgcgacaa
    11521 cggcgatctg gacgatcatg tacgatgcgt cgggcgatac agtttggacg gcgcgttacg
    11581 ggggctcaac gacctgctat gtactcagtg tgggaac
//
LOCUS       DAOPOE010000095        11113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6733_length_11113_cov_4.978206, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000095 DAOPOE010000000
VERSION     DAOPOE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_11113_cov_4.978206"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /locus_tag="PKM94_11235"
     CDS             <1..2051
                     /locus_tag="PKM94_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOD67248.1"
                     /translation="LGLMDEQAAVVARKTFLPAARLLETKPALGFDLLQPDDDRVLRR
                     QPMLTFYDGYYYPSLPLIAAAVFHNVSASAIRVTEAKSIDLGGKCAIPIDRHSAYFVN
                     FPPPGSIVNYSAADILSDTFDRRQLAGKLVLIGLDDVTSRETFTTPVAANMTKLQVQA
                     AAAENIVNADFLAVSHSTGTTLLILLAVGIVFAFLLPQVSVMYRFIILLIALVILINA
                     NYFLFSAFRVVPETVYIALELLLFLAATPLLDSQLIRGAEPAKARDEFDDIGALRNVE
                     RRTVRPEDLAHAPVRTVQENPLDHAPTTAIDYRPARTSTAPAATEALPVDHAVIDIDR
                     PADEASAPVAEEQHFDASLISDDDLEIIEAGHPAPADRSAGAPMSETVYDPPADASGE
                     WDLANQTPGGTPRPANLGRYQITGTLGRGAMGLVYRGIDPAINRPVALKTIRLDFVND
                     PEEMKELKERLYREAQAAGKLSHPNIVTIYDVGSEGPLQYIAMEYLEGRTLEEIIKKK
                     TKFNYRIFAEIIIQVCSALQYAHEQGIVHRDIKPANLMVLRDYRVKVMDYGIARIDTS
                     SMTKTGIAMGTPNYISPEQLKGQKSDHRADIFSLGVVMYEMLLGKRPFKGENITSLIY
                     AIINQEPDKPSDVNPTIPLLFDHVIMKALKKDPAQRYQRAGEIAVDLKDFVESFASGV
                     "
     gene            complement(2134..5787)
                     /locus_tag="PKM94_11240"
     CDS             complement(2134..5787)
                     /locus_tag="PKM94_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD67249.1"
                     /translation="MKQRWIIGVVVAAPLLLAGGARALEGAPPDRFGGYEPTRLIVKF
                     RAGTAVDPATVKGLATTGLPEVDALHARYGVTAQRRLVREGVPTAADNPLRSTFLFAI
                     PSGGDIEAMARDYRALASVEYAMPDYAVELYASPNDPLYVHQWALHNTGQGHYHVEDG
                     VLSIVYGTPDADIDAEEVFANPPDQTATVVVAIVDTGVDWDHPDLVSRMWQNPGEIAD
                     NGIDDDRNGYIDDLVGWDFSANDLGDPPFAIWEDNDPTDPMGHGTHCAGIVAGAIGNG
                     IGIAGAVADVRIMALKFYPYMPLSAAAAGIVYAADNGADVISMSWGLAGQIPVVEEAL
                     GYAAARGVVLIASIGNDGIEQINYPASYDLTVAVGATNDSDHVTSFSTISPYIDVCAP
                     GRSILSLRAAGTDMYAPSEPNVHIVDSQYYIASGTSMSGPYTAAVAAYVRSLSPGLDR
                     ERVREILHATADDYVDPYGTGASYPGWDKYSGWGRVNLASAIAAAPDVRAIITSPGEF
                     EIAAGTIAILGTADGADFTEYRLQYRPAAADTSAWTDLLTSTVPVTAGWLGDWNVSGL
                     PTAEYVLRLQVGQWNRDMVRVSVGGAALAEIASPGPGDTVNGGVTVTGTCFAPDFVRC
                     VIDYGAGLNPTEWHAVDTTTRVVFAGELGRWDVSLLDDGLYTLRVQVYGAAGPIAGDE
                     AVVQVYSPFAEPLGWSLDFDRKIGRIATYADVDGDGVNEIILGTATNVVFLTPDGTLK
                     TTGVPTLEGGDFTHVMPAVGRLDGDDAEDFVVMDLSGKMYICHGLSTEPTVVSLNAPT
                     MTTASYGSSANAPVLFLRDLDGDGIDEINYSAGLPSSGSFRIVRADGSPWLWCGADST
                     APSGYQECHPADLDGDGVCEIYCYGTVLRKFDAQGCSAEGDTVALVQDGLSIGRAGLS
                     LSSADLDADGDEELIVLGDSWIDPVTPVDHAIYAIDENLQSVAGWPHAMGLGAYIPVL
                     EPAFADLDGDGALEYVCANWNAIRAWRLDGSPLMGDSASFGYFCSSMEPGVVDHLVIG
                     DVTGDYRPDVAALIGGDFLSGYEVSRIEAYSDIGALQPGFPMTITSDGSGFSRCPTLC
                     DLDKDGYLDVICPSQNGINGRLVLHRFAGVRYRPEQLPSPMWRQNRRLNASAALNTGE
                     GCCVLRGDIVADGQIRVSDLTLLVGYLFRGGPAAECLAHADIDANRMVQIADLTALVS
                     YLFRGGPPPAPCG"
     gene            complement(6133..6849)
                     /locus_tag="PKM94_11245"
     CDS             complement(6133..6849)
                     /locus_tag="PKM94_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD67250.1"
                     /translation="MKKLLLGIIAAVLWGAGGSWAGPQIAVPEGEFNFGKVPQSATIC
                     HTYWLRSTGDDTLRITKIVPGCGCTKAPVEDTVLAPGDSTRIDIFFDTQRYGGYVTKK
                     PYVMTNAGEEPVFLTFHSHVLSVADSTLPIILTPNRLDVSQFTEMPRRKATFTIHNTS
                     AKDYTLTVIDCPADYFAVKLPPVVKAGETAEGSVEVLENRVGEEFERSLTFAISDEAG
                     TRYTLPVKRMVRVKKGAVGS"
     gene            complement(6932..7717)
                     /gene="lepB"
                     /locus_tag="PKM94_11250"
     CDS             complement(6932..7717)
                     /gene="lepB"
                     /locus_tag="PKM94_11250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD67251.1"
                     /translation="MVKAVNNRSAQPDLPGPEGAGGPEKAAPRARSWRPGLEILAYLL
                     VFFVVRATLVEAYKIPSSSMEDSLLIGDFIIADKITYGGEIPLLGVHLPGLREPRQGD
                     VVVFKWPVDGATKYIKRCVAVGGDTVQVVDKTLFVNGRPVPDAPGTKHTDTTSDGRPH
                     IDPADGEGFSRDNFGPYVVPAGMYFMMGDNRDNSFDSRYWGPVDASLIVGHAVLVHFS
                     WDDAANPSPAVSLSDPLSVPRLFLYNAAHFLEKVRWGRLLRTI"
     gene            complement(7831..8538)
                     /gene="lepB"
                     /locus_tag="PKM94_11255"
     CDS             complement(7831..8538)
                     /gene="lepB"
                     /locus_tag="PKM94_11255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD67252.1"
                     /translation="MSKVWDNFKQVFLAVILAILIKTSIVEAYKIPSQSMEDTLLVGD
                     FLLANKFVYGAHLPLVNWRLPALHRPEAGDVVIFIYPRDGVTKYIKRCVAVGGDTVEV
                     RNKALYVNGKPFPDPEHGKYIDTTARGEQVVQPRRPGGMDSRDNFGPFIVPPDSYFMM
                     GDNRDNSHDSRWWGAVHYRFILGEAMVIHWSWNDQAYPSPEVALTDPLSVPRVFLFNA
                     AHFFQKVRWHRLFRTIS"
     gene            complement(8671..9741)
                     /gene="rseP"
                     /locus_tag="PKM94_11260"
     CDS             complement(8671..9741)
                     /gene="rseP"
                     /locus_tag="PKM94_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOD67253.1"
                     /translation="MLLNIASFIFVLGILIFIHELGHFLLAKRVGIRVDQFSLGFPPH
                     LFKRKWGGTEYCIGVIPLGGYVKMAGENPGEERSGSPDEFMSKTVGQRAAVIFAGPFM
                     NYLLAIALLIGIAWLAGRPVFDTTRVLVGKVEKDTPAAKAGLQTGDQIIAINGEAVAN
                     FDSAAAKITPHIDAPITVTWIHAGDTVTREMVTEAQPRPNARGGIDTVGVIGFTQKAV
                     GTERVGLLTGMRHGFVEAHQLVWFTVKFVKQLITAEVSVKMVGGPVFIARESGRQAQQ
                     GAANLFYFMALLSVNLAVLNILPIPVLDGGHLLFLAIERLKGSPLSMRARVIAQQVGI
                     VAILALVLLVTYNDILRVIRGM"
     gene            complement(9732..10901)
                     /locus_tag="PKM94_11265"
     CDS             complement(9732..10901)
                     /locus_tag="PKM94_11265"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOD67254.1"
                     /translation="MNPRKLVVLGSTGSIGRSTLEVAAAHPGAFEVAGLAAFSNAALL
                     AEQYRRFRPKYLALVDERRAEQLRAAVAGEAVELRFGEAGLTELAELPGADVVVNAVV
                     GAAGLRASLAAVRRGIRLALANKESLVAGGPLFPKILKRTGAEIVPIDSEHSALWQCL
                     TAGRASEVRRLILTASGGPFRTRPRETFDRITPGEALTHPTWRMGKKITIDSATLANK
                     GLEVIEAAALFAMPAEKIAVVIHPQSIVHSMVEYIDSSIIAQLSAPDMRLPITYALFW
                     PDRAASEFGGVDPARLGTLTFEPPDIGRFPALRLAFEAARSGGTAPAVYSAANEVAVE
                     SFLQERIKFTDIAATIEEALGRIPPTADPGEDDIFEADRQARAFAGARTGKTACC"
BASE COUNT         1977 a         4175 c         3137 g         1824 t
ORIGIN      
        1 ccctcggcct catggacgag caggccgccg tggtcgcccg caaaaccttc ctcccggccg
       61 cccgcctgct ggagaccaaa cccgccctcg gcttcgatct gctccagccc gatgacgacc
      121 gcgtcctccg ccgccagccc atgctgacct tctacgacgg ctactactac ccctccctcc
      181 ccctcatcgc cgccgccgtc ttccacaacg tctccgcctc cgccatccgc gtgaccgagg
      241 cgaagagcat cgacctcgga ggaaagtgcg cgatcccgat cgaccgtcac agcgcctact
      301 tcgtcaactt ccctccgccc ggcagcatcg tcaactactc cgcggccgac atcctcagcg
      361 ataccttcga ccgcaggcaa ctggccggca aactcgtgct gatcggtctg gacgacgtga
      421 cctcgcggga aacgttcacc acgccggtcg ccgccaacat gaccaagctc caggtccagg
      481 cggccgcggc cgagaacatc gtcaacgccg acttcctcgc cgtgagccac agcaccggca
      541 ccaccctgct catcctcctg gccgtcggca tcgtcttcgc cttcctcctg ccccaggtgt
      601 ccgtgatgta ccggttcatc atcctcctga tcgcgctggt catcctcatc aacgccaact
      661 acttcctctt cagcgcattc cgcgtcgtgc cggaaaccgt ctacatcgcc ctcgaactcc
      721 tgctcttcct ggccgcgacc cccttgctcg actcgcagct catccgcggc gcggagccgg
      781 ccaaggcgcg cgacgaattc gacgacatcg gcgcgctgag gaacgtcgag cgccgcaccg
      841 tacggcccga ggatctcgcg cacgcgccgg tccgcaccgt gcaggaaaac ccgctggacc
      901 atgcgcccac gaccgccatc gactaccggc cggcccgcac ctctaccgcc cccgcggcca
      961 ccgaggccct gccggtcgac cacgccgtga tcgacatcga ccgccccgcc gacgaagcct
     1021 ccgccccggt tgcggaggag cagcacttcg acgcgtccct gatcagtgac gacgacctgg
     1081 agatcatcga ggccgggcat cccgcccccg ccgaccggtc ggccggcgcc ccaatgagcg
     1141 aaaccgtcta tgacccgccc gccgacgcct ccggcgagtg ggacctggcc aaccagaccc
     1201 cgggcggcac cccccgcccc gccaacctcg ggcgctacca gatcacgggc acgctcggca
     1261 ggggcgccat gggcctcgtt tacagaggca tcgatccggc gatcaaccgt cccgtcgccc
     1321 tcaagaccat ccgcctcgat ttcgtcaacg atcccgagga aatgaaggaa ctcaaagagc
     1381 gcctctaccg cgaggcccag gccgccggca aactctccca ccccaacatc gtcaccatct
     1441 acgacgtcgg ttccgagggc cccctccagt acatcgccat ggagtatctc gagggccgca
     1501 ccctcgagga gatcatcaag aagaagacca aattcaacta ccgcatcttc gccgaaatca
     1561 tcatccaggt ctgctcggcc ctccagtacg cgcacgagca ggggatcgtc caccgcgaca
     1621 tcaagccggc caatctcatg gtcctgagag actaccgtgt gaaggtcatg gactacggca
     1681 tcgcccgcat cgacaccagc tcgatgacca aaaccggcat cgccatgggc acccccaact
     1741 acatatcccc cgagcagctc aaggggcaga aatccgacca ccgcgccgac atcttctccc
     1801 tcggcgtcgt gatgtacgaa atgctcctcg gcaagcgccc cttcaaggga gagaatatca
     1861 cctcgctcat ctacgccatc atcaaccagg agccggacaa accgagcgac gtcaacccga
     1921 ccatccccct cctcttcgat cacgtcatca tgaaagccct gaagaaggat ccggcgcagc
     1981 gctaccagcg ggccggcgag atcgccgtcg acctgaaaga cttcgtcgag tccttcgcca
     2041 gcggcgtcta ggcgtcagcc ggccgaaaga cctgtgcccc cggtcgtcga ggcattcggg
     2101 actctcccga atgcctcgct tctcctgcat ggtctacccg cacggtgcgg gcggcggtcc
     2161 gccgcggaac aagtagctca ccagcgccgt caggtcggcg atctgaacca tgcggttcgc
     2221 gtcgatgtcc gcgtgcgcaa gacactcggc cgccgggccc ccgcgaaaca ggtaaccgac
     2281 cagaagcgtt agatccgata cccggatctg cccgtcggcc acgatgtccc cccgcagcac
     2341 gcagcaaccc tcgccggtat tgagcgccgc cgaggcattg agccgccggt tctgccgcca
     2401 catgggcgat ggcagttgct ccgggcgata gcgcacgccg gcgaaccggt gcagcacgag
     2461 acgtccgttg atgccgttct gcgaaggaca gatgacgtcc aggtacccat ccttgtccag
     2521 atcgcagagc gtcggacagc gagagaaccc gctgccgtcc gacgtgatcg tcatcggaaa
     2581 gcccggctga agtgcgccga tgtccgaata cgcctcgatc cggctcacct cataccccga
     2641 cagaaaatcc ccgccgatca gcgccgcgac atccggccgg tagtcgccgg tcacatctcc
     2701 gatgaccagg tgatcgacaa cccccggttc catcgaggag cagaagtacc cgaagctcgc
     2761 gctgtctccc atgagcgggc tgccgtccag ccgccacgcc cggatcgcgt tccagttcgc
     2821 gcagacatac tccagtgcgc cgtcgccgtc cagatcggca aacgccggct ccagcaccgg
     2881 gatgtacgcg cccaggccca tcgcgtgcgg ccatcccgcc accgactgga gattctcatc
     2941 aatcgcgtag atcgcgtgat caaccggcgt cacgggatcg atccaggaat cccccagcac
     3001 gatcagctct tcgtccccat ccgcgtcgag gtccgccgac gagaggctca gaccggcgcg
     3061 ccctatggat aacccgtcct gcaccagcgc caccgtgtcc ccctcggccg aacacccctg
     3121 ggcatcgaac ttccgcagca ccgtgccata gcagtagatc tcgcagacgc cgtcgccgtc
     3181 caggtcggcc ggatggcatt cctggtatcc gctcggcgcc gtcgaatcgg ccccgcacca
     3241 gagccaggga ctgccgtccg ccctgacgat gcggaagctc cccgacgacg ggagccccgc
     3301 ggagtagttg atttcgtcga tgccgtcccc gtccaggtcg cggagaaaca gaaccggcgc
     3361 attggcggac gaaccgtacg aggcggttgt catcgtcgga gcattcagcg agaccacggt
     3421 cggctccgtg gacagaccat ggcagatata catcttcccg ctcagatcca tgacgacgaa
     3481 atcctcggcg tcatccccgt cgagtcggcc caccgccggc atcacgtggg tgaaatcccc
     3541 gccctcaagc gtcggcacac cggtcgtctt caaggtcccg tccggagtca ggaacacgac
     3601 attggtggcc gtccccagga taatctcgtt cacgccgtcg ccgtcgacat cggcataggt
     3661 ggcgatgcgg ccgatcttcc ggtcaaaatc aagggaccat ccgagcggct ctgcgaacgg
     3721 cgagtacacc tgcacgaccg cctcgtcgcc ggcaatcgga ccggccgcgc cgtacacctg
     3781 cacgcggagc gtgtacagac cgtcgtccag cagcgaaaca tcccaccgcc cgagttcgcc
     3841 cgcaaacacg acccgggtcg tcgtgtcaac ggcgtgccac tccgtcgggt tgaggccggc
     3901 gccgtagtcg atgacacagc gcacgaaatc cggcgcaaaa caggttcccg tgaccgtcac
     3961 gccgccgttg accgtgtccc ccggcccggg tgaggcgatc tcggccaggg ccgcgccgcc
     4021 gaccgacacg cgcaccatgt cccggttcca ctggccgacc tgcagccgaa gaacatactc
     4081 ggccgtcggc aacccggaca cgttccagtc acccagccac ccggcggtga cgggaacggt
     4141 cgatgtgagc agatcggtcc atgccgacgt atccgccgcc gccgggcggt actgcagccg
     4201 gtattctgtg aaatccgctc cgtcggcggt gccgagaatc gcgatcgttc ccgccgcgat
     4261 ctcgaattct ccgggactcg tgattatggc cctcacgtcc ggcgctgcgg cgattgccga
     4321 agccagattg acccgtcccc acccgctata tttgtcccat ccggggtagc ttgcccccgt
     4381 cccgtacgga tcgacgtaat catcggcggt ggcgtgcagg atttcccgca cgcgctcccg
     4441 gtccaggccg ggcgacagcg accgcacgta agccgccacg gccgccgtgt acggccccga
     4501 catgctggtc cccgacgcga tatagtactg gctgtcgacg atgtggacat tcggctcgct
     4561 gggcgcgtac atatccgtcc cggccgcccg aagcgagaga atcgaccgcc ccggagcgca
     4621 cacatcgatg tacggactga tcgtcgaaaa ggaggtgacg tgatccgagt cattggtcgc
     4681 ccccaccgca accgtgaggt catagctcgc cgggtagttg atctgctcga tcccgtcgtt
     4741 gccgatactg gcgatcaaca ccacgccgcg ggcggcggca taaccgagag cctcctccac
     4801 caccggtatc tggcccgcaa ggccccagct catgctgatc acgtcggccc cgttgtcggc
     4861 ggcgtacacg atgccggcgg ccgccgccga gagcggcatg tagggataga acttgagcgc
     4921 catgatccgt acatccgcga ccgccccggc gatcccgata ccgttgccga tcgccccggc
     4981 cacaatcccg gcgcagtggg tgccgtgacc catcggatcg gtcggatcat tatcctccca
     5041 gatcgcaaac ggcggatcgc ccaggtcatt cgcgctgaaa tcccatccga cgaggtcgtc
     5101 aatatagccg ttccgatcgt cgtcgatccc gttgtcggca atctcccccg ggttctgcca
     5161 catccgtgac accaggtccg ggtgatccca gtccaccccg gtgtcgacaa tcgccaccac
     5221 cacggtcgcc gtctggtccg gcgggttggc gaaaacttcc tccgcatcga tgtccgcgtc
     5281 cggcgtccca tacacaatcg acagcacacc atcctcgacg tggtagtggc cctgcccggt
     5341 gttgtggagc gcccattggt gaacgtacag cgggtcgttc ggtgaggcgt agagctcgac
     5401 cgcgtaatcc ggcatcgcat actccacgga cgccagggcg cggtagtcgc gggccatcgc
     5461 ctcgatgtcg cctcccgagg gaatcgcgaa caggaacgtc gagcgcagcg gattgtcggc
     5521 cgccgtcggc accccctcgc gcaccagccg ccgctgcgcc gtcaccccgt accgggcgtg
     5581 gagggcatcg acctcgggca ggccggtcgt cgccaggcct ttcaccgtcg ccgggtccac
     5641 cgcggtcccg gcgcggaact tcacaatgag ccgggtcggc tcatagccgc cgaacctgtc
     5701 cggcggagcg ccctcgagcg cccgggcgcc gcctgccagc agcagcggcg cggccacgac
     5761 cacaccaatg atccagcgct gtttcatagg acacctcgtc tgtcgatgtg ccactgcaga
     5821 ccgtcacccg aattcccccc ttgaccttcg tgattgcgtc tttcaccggt aaaataccgc
     5881 cgtaacagct cgtgtcaacg tgttttcccg ccggcaccgg agagcgccgt tctgagtctc
     5941 ccgcttcggc ttcttgcccg agtctctgtg agacggagcg ctttcgatgc ctgtctgtgg
     6001 gcagcaaatc gagcgcccat gtggccaacg cgttcggtcg acgccccgcg cgtatgaacc
     6061 ggccgcccgc aaaaaagccc ggccgggctc tgggccccgc cgggctgtcc aaactgcgct
     6121 ttctgctctc cctcagctcc ccaccgcccc tttcttgacc cgcaccatcc gcttcaccgg
     6181 cagcgtgtag cgcgtccccg cttcgtcgct gatcgcaaac gtcagcgacc gctcgaactc
     6241 ctcccccacc cggttctcga gcacctctac cgacccctcc gcggtctccc ccgctttcac
     6301 caccgggggc agcttcacgg cgaaatagtc ggccggacaa tcgatcaccg tcagcgtgta
     6361 atccttcgca ctcgtgttgt gaatcgtaaa cgtcgccttc cggcgcggca tctcggtgaa
     6421 ctgcgacaca tccaggcggt tcggcgtgag aatgatcggc agcgtgctgt cggctaccga
     6481 cagcacatgc gagtggaagg tgagaaacac cggctcctcg ccggcgttcg tcatcacgta
     6541 cggcttcttg gtcacatacc cgccgtaccg ctgggtgtcg aaaaagatgt cgatccgggt
     6601 gctgtcgccg ggggccagaa ccgtgtcctc caccggcgct ttggtgcacc cgcaaccggg
     6661 cacgattttc gtaatccgca gcgtgtcatc cccggtggaa cgcagccagt aggtgtggca
     6721 gatggtcgcc gactgcggca ctttgccgaa attgaattcc ccttccggca cagcgatctg
     6781 cgggccggcc cacgaacccc ccgcccccca cagcaccgcg gcgatgatcc cgagaagcag
     6841 cttcttcatt gtgcgttcat cctccggctt tggtcaggat ctattctctg gtcggacgcc
     6901 ggaccgcggg gcgcgccccg gcgcttcatt ctcatatcgt gcggagcagc cgcccccagc
     6961 gcaccttctc cagaaagtgc gccgcattat acagaaacag ccgcggcacc gacagcggat
     7021 ccgaaagcga gaccgccggc gacggattcg ccgcatcgtc ccacgaaaaa tgcacgagca
     7081 ccgcgtgccc gacaatcagg ctcgcgtcca ccggccccca gtagcgcgaa tcgaacgagt
     7141 tgtcccggtt gtcccccatc atgaaataca tccccgccgg cacgacatac ggaccgaaat
     7201 tgtcccggct gaatccctcc ccgtcggccg ggtcgatgtg cggacgcccg tcgctcgtcg
     7261 tgtccgtgtg tttcgtcccc ggcgcatccg gcaccggacg cccattgacg aacagcgtct
     7321 tgtctaccac ctgcaccgtg tctccgccca ccgccacgca ccgcttgata tatttcgtcg
     7381 ccccgtccac cggccacttg aacaccacca catccccctg ccgcggctcg cgcaaaccgg
     7441 ggagatgaac ccccagcaga ggaatctccc cgccgtaagt tatcttgtcg gcaatgatga
     7501 aatcgccgat cagcaggctg tcttccatcg acgacgacgg gatcttgtac gcctccacga
     7561 gggtcgcccg gacgacaaag aaaaccagca gataggccag gatctccagg ccgggccgcc
     7621 aggagcgggc gcgaggggcc gctttttccg gaccgcccgc gccttccggc ccgggaaggt
     7681 cgggttgcgc ggacctgtta ttaaccgctt tcaccatacc tgattgccgc ttctgatttc
     7741 gtattacgct ttataccgcc gctgccgcgc ccggttcgcc ccgtttccgg cctcccccgc
     7801 cgcgcccgga cccggattac ctcggcccgc tcaactgatg gtccggaaca accggtgcca
     7861 ccgcactttc tggaagaaat gcgccgcgtt gaacagaaac acccgcggca ccgacagcgg
     7921 atcggtcagc gccacctccg gcgagggata cgcctggtcg ttccacgacc agtggatgac
     7981 catcgcctcg cccaggatga accggtagtg caccgccccc caccaccggg aatcatgcga
     8041 gttgtcccgg ttgtccccca tcatgaaata gctgtcgggc ggaacgataa agggtccgaa
     8101 attgtcccgg ctgtccatcc cgcccggacg gcgcggctgg accacctgct ccccccgggc
     8161 ggtggtgtcg atgtacttcc cgtgctccgg atcgggaaac ggcttcccgt tcacgtagag
     8221 cgccttgttc cgcacctcca ccgtgtcccc ccccacggcc acgcaccgct tgatatactt
     8281 cgtcaccccg tcgcgcgggt agatgaaaat cacgacgtcc ccggcctccg gacggtgcag
     8341 cgccggcagc cgccagttca ccagcggcag gtgcgccccg tagacaaact tgttcgccag
     8401 aaggaaatcc ccaaccagga gcgtgtcctc catcgactgc gacggaatct tgtaggcttc
     8461 cacgatcgag gttttgatga ggatcgccag gatgacggcc aggaagacct gcttgaagtt
     8521 gtcccacact ttgctcatgc gcgctttccc agttgtgccg gcgttccgcc gtcgtggtcc
     8581 tcgccccgat ggtcctccgg ggcgccttca tataagcgat tgtacgccgt ttgccaaggt
     8641 ttgttttgcg ccgcgccgcc ccgccccggc ctacatcccg cggatcaccc ggaggatatc
     8701 gttgtaggtc accagcagaa cgagcgccag aatcgccaca atccccactt gctgcgcgat
     8761 cacccgcgcc cgcatcgaca gcggcgagcc tttcagccgc tcgattgcca aaaacagcaa
     8821 atgcccgccg tccagcaccg ggatcggcag gatgttaagc accgccaggt tcaccgagag
     8881 cagggccatg aagtagaaca ggttggcggc cccctgctgc gcctgccggc ccgactcccg
     8941 cgcgatgaat accggccccc ccaccatctt gaccgagacc tccgccgtga tcagttgctt
     9001 gacgaacttg accgtgaacc acaccaactg gtgcgcctcg acaaatccgt gccgcatccc
     9061 ggtgagcagc ccgacccgct cggtgccgac cgccttctgg gtgaacccga tcacccccac
     9121 ggtgtcgatg ccgccccggg cattgggccg cggctgcgcc tccgtcacca tctcccgcgt
     9181 caccgtgtcc cccgcgtgga tccaggtgac cgtgatcggg gcatcgatgt ggggcgtgat
     9241 cttggccgcc gccgaatcga aattcgccac cgcctcgccg ttgatcgcga tgatctggtc
     9301 gccggtctgc agcccggctt tggccgccgg cgtgtccttc tccaccttgc ccaccagcac
     9361 gcgcgtcgtg tcgaaaaccg gccgcccggc cagccaggca atgccgatga gcagcgcgat
     9421 cgccagcagg tagttcatga agggcccggc gaaaatcacc gccgcccgct gccccaccgt
     9481 cttggacatg aactcgtcgg gcgaccccga ccgctcctcg cccgggttct cccccgccat
     9541 cttcacatac cccccgagcg ggatcacgcc gatgcagtat tccgtcccgc cccacttccg
     9601 cttgaacagg tgcgggggga agcccagcga aaactggtcg acccggatcc ccacccgctt
     9661 ggccaggaga aagtgcccga gctcgtgaat gaaaatcaga atccccagca cgaagatgaa
     9721 agatgcaatg ttcaacagca tgccgttttc cctgtcctgg cgccggcgaa cgcccgcgcc
     9781 tgccgatcgg cctcaaaaat gtcgtcctcg cccgggtccg ccgtcggcgg tatccgcccg
     9841 agcgcctctt cgatggtagc tgctatatcg gtaaacttga tgcgttcctg caagaaactt
     9901 tccaccgcca cttcgttcgc cgcgctgtac accgccggcg ccgtcccccc gctccgcgcc
     9961 gcctcgaacg ccaggcgcag ggccgggaac cgcccgatat ccggcggctc aaaagtcagc
    10021 gtccccaggc gcgccggatc cacccccccg aactccgagg ccgcccggtc cggccagaac
    10081 agcgcgtacg tgatcggcag ccgcatgtcg ggcgccgaca actgcgcgat gatcgacgag
    10141 tcgatatact cgaccatgct gtgcacgatc gactgcgggt gaatcaccac cgcgatcttt
    10201 tccgccggca tcgcgaacag cgccgccgcc tcgatcacct cgagcccctt gttcgccagc
    10261 gtcgccgaat cgatcgtgat cttcttcccc atccgccagg tcgggtgcgt gagcgcctcg
    10321 ccgggcgtga tccggtcgaa cgtctcccgc ggccgcgtcc ggaacggtcc ccccgacgcc
    10381 gtgaggatca gccgccgcac ctcgctcgcc cgccccgccg tcaggcactg ccacagcgcc
    10441 gagtgctcgg agtcgatcgg gacaatctcc gcgcccgtcc gcttcagaat tttcggaaag
    10501 agcggcccgc cggccaccag cgactccttg ttcgccaacg ccagtcggat cccccgccgg
    10561 accgcggcca gcgaggcccg cagccccgcc gcccccacca ccgcgttgac caccacgtcg
    10621 gcgccgggaa gttccgccag ctcggtcagc cccgcctccc cgaaacggag ttccaccgcc
    10681 tcgccggcca ccgccgcgcg cagttgctcc gcccgccgct catcgacgag cgcgagatat
    10741 ttcggccgga agcggcggta ctgctccgcc aacagcgctg cgttgctgaa agccgccagc
    10801 cccgccacct cgaaagcccc ggggtgggcg gccgccacct ccagggtcga gcggccgatc
    10861 gagccggtcg agccgagaac cacgagtttg cgcggattca tcgtcgccta atagaacggc
    10921 cggcccctgc gccggttcca ctaaaacatt ccgcaaaaag caacggcggg gcgccggggc
    10981 cccgccgtcc caatcgagtc atccccccgc tcagttctgc ttgagcggct tgccctccgc
    11041 gtcggagaca aagacctcgc gcatgtagac cgtctcggcc ggcttcgaat tctccccccg
    11101 gaacgccgtc acc
//
LOCUS       DAOPOE010000096        10828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_6944_length_10828_cov_4.836072, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000096 DAOPOE010000000
VERSION     DAOPOE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_10828_cov_4.836072"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..2046
                     /gene="gatE"
                     /locus_tag="PKM94_11270"
     CDS             37..2046
                     /gene="gatE"
                     /locus_tag="PKM94_11270"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOD67255.1"
                     /translation="METEYIAGQQTGPVDPDANIRQTAALVQYVPRNRATRETYAALG
                     FKCGLEIHQQLLTRSKLFCRCPAGIYHHRDDFDAEVIRHMRPTLSEMGEYDGTALMEK
                     RTKKNIIYRIKDRTACTYEIDDTPPFKIDREALEIAIEVALLLKTNIVGELHITRKQY
                     LDGSIPTGFQRTAIVGIEGEIPLAARKVRIIQLSVEEDSCREVSDVAHDRTYTTDRLG
                     MPLIETVTYPDMLTPDEAAEAAQYLRYLARSTGHVRTGIGAAREDVNVSITGGTRVEI
                     KGVAKIKWIPELTHNEAFRQRALLEIARILRERVGDPAAWRISQADLPFAVFEQPYAV
                     WASARQRGGRLAVINLPDFKGILSFFTQPGQMFAHELEGRVKVIACLERPNIVHSEQP
                     DLVLSAADWAAVYERLQPGPGDAQVLVWGPPADVATAIETIEERCRMAFDGVPNETRK
                     ALPDGTTIFERVLPGPDRMYPDTDSAPLSVSEELIERLRRRLPVEVAERMRQLSTWQV
                     PADAHRYLLARNLTPLIERIASDWSYEPRWVGKVLGHGLKNIERENISEGAPLDYERL
                     YGLFAFVKEQGLDREIIKAMLPAAYRNPQAGFAEILERTGFRRRSEGDLLGRIPGLVG
                     QFRRHRRSGHPEAAIRWAMGVLRPEALGNLSFAKLRKAIAEEVRV"
     gene            2043..3428
                     /gene="gatD"
                     /locus_tag="PKM94_11275"
     CDS             2043..3428
                     /gene="gatD"
                     /locus_tag="PKM94_11275"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOD67256.1"
                     /translation="MTEDILKGYRGAAREVLEKYRIRVWSQTEINTTRGGFAGILLPR
                     SVNDDENHLVLKLATGYNIGIDVATILDMKESGYKEAHYKIPEKEFPVSPGKPHVKLL
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLSTEKLFAVFSENMGPYQYR
                     ELAKAIGREIEKGIDGIIIGHGTDTMHHTASALAFMVQNPPVPIVMVGSQRSSDRPSS
                     DAALNLIHATKTAAESDIAEVMVCMFGPTSDEYALLHRGTRVRKMHSSYRSTFRTVGD
                     TPLAMVDRQRITPLKDTYNRRRRDRQVKVLPYFEEMVALVYYYPNMRPDIIDSLRENG
                     YRGIVIAGTGLGHINKPVYPAIERAAQAGIAIYMTVQTLWGYVHMFVYDTGRDLMSLG
                     IIPAENMLPEVAYIKLGWALGQTEDLNEVKRLMLTPIAEEITPREPHNGYLIFQGGVP
                     EVEEFLKKIHK"
     gene            complement(3525..4481)
                     /locus_tag="PKM94_11280"
     CDS             complement(3525..4481)
                     /locus_tag="PKM94_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD67257.1"
                     /translation="MPYNFALTGASGYVAPRHLKAIKETGNRLIAAVDPHDSVGILDG
                     YFDRVAFFTEFERFDRHIEKLRRLGSEKAVHYVSICSPNYLHDAHCRFALRVGANAIC
                     EKPLVLNPWNIDALKELEEESDRRIYTILQLRVHPALIALRARLQAQPANAKNDVRLT
                     YITSRGPWYLVSWKGQIERSGGLATNIGIHFFDMLMWFFGGVRRSAVHLANPLQTAGF
                     LELDRARVQWFLSINQDDLPDSAKAKGQRTYRSITIDGEEIEFSEGFTDLHTLVYRET
                     LAGRGFGLEDARPSIELVHDLRAAVPVGLNDDAHPCARRCAP"
     gene            complement(4689..6062)
                     /locus_tag="PKM94_11285"
     CDS             complement(4689..6062)
                     /locus_tag="PKM94_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOD67258.1"
                     /translation="MSTRNRQAALAVLLLCLFGLSAGGADAVNYSVANRGRVADNASE
                     IIMPADGSPKAPFSGALRLYVVEPESRWDDADGLPYHFATIDIALDTSIALNDGDRYV
                     RSRFWEGTTWAMTEQNAMVIAVLFEGTPHTAYSDPPSGGVFNAYYAEAAAYARPGFCD
                     SNNTSGNSTHTVFIEEGTAVWCPYCPDPNYWLYYVYNQGVLNFIYTALVDDRNSDAQK
                     RLDSYNIIYFPTCYLDGGNQILIGGYTAASYYQNAIAAADSRVVSGANVIVKVTWMGN
                     SDLQVDYAIAKGTPLNTGTANPGPPTGEVEPLIGTPHLYTTGVTDPEGDQMWYQFDWG
                     DGEQSAWQGPYASGDPCSQSHQWAAVGAYDVRVKARDLWLAETAWSDPLTVNAQCCQL
                     RGDFNSDGVVKVADLTALINYLFRGGVAPVCLDHGDTSGDGTIKVGDLTILINYLFRG
                     GPAPAAC"
     gene            complement(6127..7338)
                     /locus_tag="PKM94_11290"
     CDS             complement(6127..7338)
                     /locus_tag="PKM94_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67259.1"
                     /translation="MKPACLGPAVLLAALVLYPPSSRADAEIEFGLNNGYTNNLLSEA
                     SAQDDAYSTARLTARWYPLPQLQLSLGTDYTYYNNVIGLGNNTPRAGLIWVPTDPAGR
                     LTFYLEGNTSARRYRAAYSSYSTNEGYLKGSVGFRLNPRFLLRSGIKFNGTNYITPDS
                     TTDADYEQYELFAGVNIALPWSNSFDLEAGLGQTNFALIDAAKNPDFQNPYAAPGVQW
                     PATKFLEDGDFRALYVSPRVSRPLGDKTGISLTYVYRQFLDVDEAVVLGITTDFLSPW
                     TSFFDGSSLQLRIKSYVISDFILTGGLGYWDKTFLRSTEVVVDTIPTPRPDDPDRVRI
                     ETRWVDPKDATAREDDMTRLYFGLQRPFTFRGGLYLEPSLGLEYIDNRSTRDNFDYST
                     TAVTFNILVRP"
     gene            complement(7352..7720)
                     /locus_tag="PKM94_11295"
     CDS             complement(7352..7720)
                     /locus_tag="PKM94_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67260.1"
                     /translation="MASATDTPAPVPAAPPAAPILFVDFDGDGINDNFVDANRDGIPD
                     EPEPIASTAGIFSGDTRFASPSFALAARNSDVFNQRLSRLTCLTLNRGGFGSAENFGP
                     GNGISAGAVSSGTCVGGVCH"
     gene            complement(7987..9102)
                     /locus_tag="PKM94_11300"
     CDS             complement(7987..9102)
                     /locus_tag="PKM94_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD67261.1"
                     /translation="MKHTTLLLLALAALALCVAGCDREITGDVEMVNQPAAGCFDCHS
                     DQDFDLAQVAAQYDLSAHAHSATVNENRLNGSQGCERCHTNEGFVAFVTGVPADGDYF
                     TNFACFTCHAPHSTGKFGVRVETAVTLENGAEYDEGASNLCATCHHSRRSVNVYITDP
                     ETFGSTHWGPHGSPQADMYIGTNGYEYDDWDYSPSRHPSVIEDGCVACHMADPALWAP
                     VGGHTFNIAGEVEVEDEPGMFEEVTNFANSCGGCHESFATINPVFPADIDYDYDGTSE
                     GVQDEIHGLLDSLGVLLLNAGLVIEEDGLLEPDPEVTTSADSAGAVWNYLFVESEGSH
                     GIHNTNYAVALLQSAIAYLAGDTGPALAGRPSLFSAH"
     gene            complement(9174..>10828)
                     /locus_tag="PKM94_11305"
     CDS             complement(9174..>10828)
                     /locus_tag="PKM94_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67262.1"
                     /translation="RMPMKLCILTPAVVVLVSLLAPAPAFAADYSGTLRAGYVYSDED
                     GNRGVFQPTYNLYEGAALSLKDFYYAFDNGLRLYADLDNVTLNDRTILVGATKARLFG
                     LSVRNSQYRRTYSFTADRFTRRNATRTDGWLQALPWLKLFGGLGYVDKHGTVVKSVAA
                     PPLGLPTYRVDYDHFFYNAGVTVGGNRRFATLEYRGSDFSDGPSLTATDRASRRYRLT
                     AYGPVPGFEKLVLHGGAQRFEHSLTTRDDSLVATTVWGGGRYSFGDGWGVRYNAVWNR
                     ARSTDDLASTDNITHAVFGDKRFGTFAGATAGYRYRYNDDIRNERRADGFVVSGWIRP
                     VPALTVRAGYGADMLDTRAGDPLIREDETTRGWASVKYRHPRVTWQAKVEHQSRDRVL
                     DDRSLDEGGGIINVTASDTTIATEVDFVRVASDLTADLPRYGQVSASYAYYHGDYANA
                     GGDCFAFEEHVVSGDLLTAPCRGFRVGVGGQYYRTRLDQDIESFSLRLRGVYEFLKHY
                     AVEAVYAAHNYDDFADLGGLYDQYYTANVVQVSLVYDFGAQR"
BASE COUNT         2030 a         3116 c         3862 g         1820 t
ORIGIN      
        1 gttgccctgc ggcggcgcga ttgacaggtg gtcgcaatgg agacggaata catcgccggg
       61 cagcagaccg gtccggttga cccggacgca aacatccggc agacggccgc gctcgtgcag
      121 tacgtcccgc gcaaccgggc gacgcgggaa acctatgcgg cgctcgggtt caagtgcgga
      181 ctggaaattc atcaacagct tcttacccgg agcaagctct tctgccgctg tccggcgggc
      241 atctaccacc accgggatga ctttgacgcc gaggtcatcc ggcacatgcg cccgacgctg
      301 tcggagatgg gggagtacga cggcacggcg ctgatggaaa agcggacgaa gaagaacatc
      361 atttaccgga tcaaggaccg gaccgcctgc acctacgaaa ttgacgacac gccgccgttt
      421 aagatcgacc gggaggcact ggaaatcgcg atcgaggttg cgcttctgct gaaaaccaac
      481 attgtcggcg agcttcacat aacgcgaaag cagtacctgg acgggtcgat cccgaccggt
      541 tttcagcgca cggcgattgt cggcatcgag ggggaaattc ccctggcggc caggaaggtg
      601 cggatcatcc agttaagcgt cgaggaggac agctgtcggg aagtgtcgga cgtcgcccac
      661 gaccggacct acacgaccga ccggctgggg atgccgctga tcgaaacggt gacatatccg
      721 gacatgctga cgcccgacga agcggcggaa gcggcccaat acctgcggta tctcgcgcgg
      781 agcacgggtc acgtgcggac cgggatcggg gcggcccgag aggatgtgaa tgtgagcatc
      841 accgggggaa cgcgggtgga aatcaagggg gtggcgaaga tcaagtggat accggaattg
      901 acccacaacg aggcgtttcg ccagcgggcg ctgctggaga tcgcccggat tctgcgggag
      961 cgggtgggtg atccggccgc ctggcggatc agccaggcgg acctgccctt cgccgtcttc
     1021 gagcagccct acgcggtctg ggcgagcgcg cgccaacgcg gggggcggct ggcggtcatc
     1081 aacctgccgg atttcaaggg gatactctcg ttcttcaccc agccggggca aatgtttgcg
     1141 cacgaactgg aggggcgggt gaaagtgatt gcttgcctgg agcgcccgaa tattgtgcac
     1201 agcgagcagc ccgacctggt gctctcggcg gccgactggg cggccgttta cgaacggctg
     1261 caaccggggc cgggggatgc ccaggtcctg gtgtgggggc cgccggccga cgtagccacg
     1321 gcaatcgaga cgatcgaaga acggtgccgg atggcttttg acggcgtgcc gaacgaaacg
     1381 cggaaagcgc tgccggacgg gacgacgatt tttgagcggg tcctgccggg accggaccgg
     1441 atgtacccgg acaccgactc ggcgccgctg tcggtgtcgg aggagctgat cgagagactg
     1501 cggaggcgcc tgccggtcga ggtggccgag cggatgcggc agttgagcac ctggcaggtg
     1561 ccggcggacg cacaccggta tctgctggcc cgcaatctga caccgctcat cgagcggatc
     1621 gcgtccgatt ggagctacga gccgcgctgg gtcggcaaag tgctcggcca cggactgaaa
     1681 aacatcgaac gcgagaatat ctcggaaggg gcgccgctgg attatgaacg gctgtacggg
     1741 ctgttcgctt tcgtgaagga gcaggggctg gaccgggaga tcatcaaggc aatgctcccg
     1801 gcggcctacc gcaacccgca ggccggtttt gcggagattc tggagcgcac gggattccgc
     1861 cggcgaagcg agggcgacct gctcgggcgg attcccgggt tggtgggcca attccgaagg
     1921 caccgccggt cgggccaccc ggaggccgca atccggtggg cgatgggcgt gctgcggccg
     1981 gaggcgctgg ggaacctgtc gttcgccaaa ctccggaagg ccatcgccga ggaggtgcgg
     2041 gtatgacgga agatatcctg aagggatacc ggggggcggc ccgcgaggtg ctcgaaaagt
     2101 accggattcg ggtctggagc cagacggaga tcaacacgac ccggggcggg ttcgcgggaa
     2161 ttctgttgcc ccggtcggtc aacgacgacg agaaccacct ggtgctgaag ctggcgacag
     2221 ggtacaacat cgggatcgac gtggccacca tcctggacat gaaagagagc gggtacaagg
     2281 aggcccacta caagattccg gagaaggagt tcccggtttc gccggggaaa ccgcatgtga
     2341 agctgctggg gaccgggggc acgattgcgt cgcggctgga ctaccggacg ggggcggtga
     2401 ttccggcctt ttcgccgggg gagctgtacg gggcggtgcc ggaattggcg gacatctgca
     2461 acctgtcgac ggagaagttg tttgcggtgt tcagcgagaa catggggccg taccagtacc
     2521 gggagctggc caaagcgatc gggcgggaga tcgagaaggg gatcgacggc atcattatcg
     2581 gtcacgggac ggacacgatg caccacacgg cgtcggctct ggcgttcatg gtgcagaacc
     2641 cgccggtccc gatcgtgatg gtaggctcgc agcggtcgag cgaccggccc tcctcggacg
     2701 cggccctcaa cctgatccac gcgactaaga cggcggcgga gtcggacatc gccgaggtga
     2761 tggtgtgcat gttcgggccg acctccgatg agtacgcgtt gctccaccgg ggcacgcggg
     2821 tgcgcaaaat gcactcgtcg taccggtcga cgtttcggac ggtgggggac accccgctgg
     2881 cgatggtcga ccggcagcgg atcacgccgc tcaaagacac ctacaaccgg cgtcggaggg
     2941 accggcaggt gaaggttctg ccgtatttcg aggagatggt ggcgctggtc tactactacc
     3001 ccaacatgcg gccggacatc atcgactccc tgcgcgagaa cggctaccgg gggatcgtga
     3061 tcgcgggcac gggcctgggg cacatcaaca agccggtgta cccggcgatc gagcgggcgg
     3121 cccaggccgg catcgcgatc tacatgacgg tgcagacgct gtgggggtac gtccacatgt
     3181 tcgtgtacga cacggggcgc gacctgatga gcctcgggat catcccggcc gagaacatgt
     3241 tgcccgaggt ggcgtacatc aaactcgggt gggcgctggg ccagacggag gacctgaacg
     3301 aggtgaaacg gctgatgctg acgccgatcg ccgaggagat tacgccgcgc gagccacaca
     3361 acgggtacct gattttccag ggaggcgtcc ccgaagtgga ggagttcctg aagaagattc
     3421 acaagtaagt gagcggggat ctcggcgcgt gtcagacctc aaacgccgaa agccgccggc
     3481 cagccaccgg cggctttgct attgagggca atcggccggg agcgtcacgg ggcgcagcgg
     3541 cgggcgcacg ggtgggcgtc gtcgttgaga ccgaccggaa ccgccgcgcg caggtcatgc
     3601 accagttcaa tcgaggggcg ggcgtcctcg aggccaaacc cccggcccgc gagcgtctcc
     3661 cggtacacca gagtgtgaag gtcggtgaag ccttcagaga attcgatctc ctcgccgtcg
     3721 atagtgatgg agcggtaagt gcgctgtccc ttggccttgg ccgaatcggg gaggtcatcc
     3781 tggttgatcg agagaaacca ctggacgcgg gcgcggtcga gttcgaggaa accggccgtc
     3841 tgcagcgggt tggcgaggtg gacggcggag cggcggacgc cgccgaaaaa ccacatgagc
     3901 atgtcgaaga agtgaatgcc gatgttcgtg gccaaaccgc cggaacgctc gatctgcccc
     3961 ttccaggaaa ccaggtacca gggaccgcgc gaggtgatgt aggtgaggcg tacgtcgttc
     4021 ttggcgttcg ccggctgcgc ctggagacgg gcgcgcaacg cgatcagggc cggatggacg
     4081 cgcaattgga ggatcgtgta gatgcggcgg tcactctcct cctcgagctc cttgagggcg
     4141 tcgatgttcc acgggttgag cacgagcggt ttttcgcaga tggcgttggc gccgacccgg
     4201 agggcgaacc ggcagtgcgc gtcgtggaga taattggggg aacagatgga gacgtagtgc
     4261 accgccttct cggaaccgag gcggcgcagt ttctcgatgt gccggtcaaa gcgttcgaac
     4321 tcggtgaaga aggcgacccg gtcgaaatag ccgtcgagaa tgcccacgct gtcgtggggg
     4381 tcgacggcgg cgatgaggcg gttgccggtc tccttgatcg ctttgaggtg gcgcggggcg
     4441 acatacccgg aggcgccggt gagagcaaag ttgtagggca tggagttcct caacgtgatt
     4501 ccgtaacgat ccagtggtct gtcaatcctc caataaacgg cccctcgcgg gaggcgcaag
     4561 gacgaaattg cggcggtcgg ggcggccgcc gtcacaaaaa agcgccgggc catccacgca
     4621 acgcgcgggt ggcccgacgg atctttcaga ccccgccgaa ggcgatggtc cgcggagtcc
     4681 gatgctgctc agcaggcggc cggggcggga ccgccgcgga agaggtagtt gatcagaatc
     4741 gtgaggtccc cgactttgat cgtgccgtca ccggaggtat cgccgtggtc gaggcagacc
     4801 ggcgccacgc cgccgcggaa gaggtaattg atcagggcgg tcaggtcggc gaccttgacg
     4861 accccgtcgg agttgaagtc gccgcggagc tggcagcact gcgcgttcac ggtcagcgga
     4921 tctgaccagg ccgtctcggc cagccacaga tcgcgcgcct tgacgcggac gtcataggcg
     4981 cccacggccg cccactggtg ggactgggag cacgggtcgc cggaggcgta cgggccctgc
     5041 caggccgact gctcgccgtc gccccaatca aactggtacc acatctgatc cccctcgggg
     5101 tcggtcaccc ccgtggtgta cagatgggga gtgccgatga ggggctccac ttcgccggtc
     5161 ggcggacccg ggttggccgt gccggtattg agcggcgtcc ccttcgcgat ggcgtagtcg
     5221 acctgcagat cgctgttgcc catccaggtg actttcacga tgacgttggc gccgctgacg
     5281 acgcggctgt cggcggcggc gatggcattc tgatagtagc tggcggcggt gtagccaccg
     5341 atgaggatct ggttgccgcc gtcaaggtag caggtcggga agtaaatgat gttgtaggaa
     5401 tccagccgct tctgggcgtc gctgttgcgg tcgtcgacca gagccgtata gatgaagttc
     5461 aggacgccct ggttatagac gtagtagagc cagtagttcg gatccgggca atacggacac
     5521 cagaccgccg tgccttcctc gatgaacacg gtgtgggtgg agttgccgga ggtgttgttg
     5581 ctgtcgcaga aaccggggcg ggcgtaggcc gccgcttccg cgtagtacgc gttaaagact
     5641 ccgccggacg gcggatcgct gtaggcggtg tgcggggtgc cctcaaacag caccgcgatc
     5701 accatcgcat tctgctccgt catcgcccag gtcgtcccct cccagaagcg gctgcggaca
     5761 tagcggtcgc cgtcgttgag ggcgatggac gtgtcgaggg cgatgtcgat ggtggcgaag
     5821 tggtagggga gtccgtcggc gtcgtcccag cgcgactccg gctccacgac atagaggcgc
     5881 agggcgccgc tgaagggcgc cttgggcgat ccgtcggcgg gcatgataat ctcggacgcg
     5941 ttgtcggcca cgcgccccct gttcgcgacg ctgtagttga cagcgtctgc gccgccggcc
     6001 gaaaggccga acaggcacaa cagcagcacg gcaagggctg cctgacggtt gcgggtactc
     6061 attccgggat cctttctcgt caatcggaca atcggtggtt ctgttgccaa ggtaccgtta
     6121 acggcctcac ggccggacaa ggatattgaa ggtcacggcc gtggtcgaat agtcgaaatt
     6181 gtcgcgcgtg gagcgattgt caatgtactc caggcccagg ctgggttcga ggtacaatcc
     6241 gccgcggaac gtgaacgggc gctggagacc aaaatagagc cgggtcatgt cgtcttcgcg
     6301 ggcggtcgcg tccttgggat ccacccagcg cgtttcaatc cggacgcggt cggggtcgtc
     6361 ggggcggggc gtcggaatgg tgtccactac cacctcagtc gagcgcagga aggtcttatc
     6421 ccagtagccc aggccgccgg tcagaatgaa atcggaaatc acatagctct taatgcgcag
     6481 ttgcagcgag gagccgtcga agaaacttgt ccacggcgac aggaagtcgg tcgtgatgcc
     6541 gagaaccacg gcttcgtcga catcgaggaa ctgccggtaa acgtaggtga ggctgatgcc
     6601 ggttttgtct ccgagcggcc gcgagacgcg cggagagacg tagagagcac gaaagtctcc
     6661 atcctcaagg aacttggtgg ccggccactg cacgccgggc gcggcgtagg ggttctgaaa
     6721 atccgggttc ttggccgcgt cgatcagagc aaagttggtc tggcccaggc cggcttcaag
     6781 atcgaaggag ttactccacg gaagagcgat gttgacgccc gcaaagagct cgtactgctc
     6841 gtagtcggca tcggtcgtgc tgtccggggt tatgtagttc gtgccgttga acttgatccc
     6901 cgatcggagg aggaagcggg ggttgagccg gaatccgacc gagcccttca agtagccttc
     6961 attggtgctg taggaggagt acgcggcccg ataccggcgc gcgctggtgt tgccctcaag
     7021 gtagaaagtg aggcggccgg ccgggtcggt cggcacccag atcaagccgg cgcgcggggt
     7081 gttgttgccg aggccgatga cgttattgta gtaggtataa tcggtgccga ggctgagctg
     7141 gagctggggg agggggtacc acctggcggt gaggcgggct gtggagtaag catcgtcctg
     7201 ggccgaggcc tcgctcagga ggttgttggt gtaaccgttg tttaggccga attcgatctc
     7261 cgcatccgcg cgcgaggaag ggggatacag cacgagcgcc gccagaagaa cggccgggcc
     7321 gagacaggca ggtttcacgt ttccgcgcct ttcagtggca cacgccgcca acgcacgtgc
     7381 cgctgctgac cgcgccggcg ctgatgccgt taccgggacc gaaattctcg gccgaaccaa
     7441 acccgccgcg gttgagcgtg aggcaggtca ggcggctgag ccgttggttg aaaacatcgg
     7501 agttgcgggc cgccagggca aacgatggag aggcaaaccg ggtgtcgccg gagaagatgc
     7561 cggcggtcga agcaatcggc tcgggctcgt ccgggatacc gtcgcgattg gcgtcgacga
     7621 aattgtcatt gatgccatcg ccgtcaaaat cgacaaagag aatcggagcg gcaggagggg
     7681 cggcaggaac aggggccggg gtgtccgtcg cggatgccac actgacgagt atcggaaaca
     7741 caaccgagag cactgagcga attcccaaag acgtctcctt tcgcgatgcg agtctcagca
     7801 caactcaatt tgcgacgagc ggatcgcggc tgtatccaat acttgataat aaggtacatt
     7861 tgaccaaatt ccaaactatt tctggggttt tttgcggtag tgcccccaat cgcaaagatt
     7921 gacgacgcgt aacgggaaag cccctccggg gggaggggcc ttcgcgcaaa agcgggacga
     7981 aacctgtcag tgggccgaga acagggaggg ccgtccggcc aaagccggac cggtatcgcc
     8041 ggccaggtag gcaattgccg actggagcag ggccaccgcg tagttggtgt tgtgaatgcc
     8101 gtgcgagcct tcgctctcaa caaacaggta gttccagact gccccggccg agtccgccga
     8161 cgtggtcacc tcggggtcgg gctcgagcag gccgtcttcc tcgatcacca ggccggcgtt
     8221 gagcaggaga acgccgaggc tgtcgagaag accgtggatt tcgtcctgaa cgccctcgga
     8281 ggtgccgtcg tagtcgtagt cgatgtcggc cgggaagacg gggttgatcg tggcgaagga
     8341 ctcgtggcag ccgccgcagg agttagcgaa attggttact tcttcaaaca tccccggctc
     8401 atcctcgact tcgacttcac cggcgatgtt gaaggtgtgg ccgccgacgg gggcccagag
     8461 ggcgggatcg gccatgtggc aggcgacgca gccgtcttcg atgacgctgg ggtggcggga
     8521 cgggctgtag tcccagtcgt cgtactcgta cccgttggtg ccgatataca tgtcggcctg
     8581 cgggctgccg tggggtcccc agtgggtgct gccgaaggtc tcggggtcgg tgatgtagac
     8641 gttcacgctg cgccgggagt ggtggcaggt cgcacagagg ttggacgcgc cctcgtcgta
     8701 ctcggcgcca ttttccagcg tgaccgccgt ctccacgcgg acaccgaact tgcccgtgct
     8761 gtgcggggcg tgacaggtga agcaggcgaa attggtgaag taatcgccgt cggccgggac
     8821 gccggtgacg aaggcgacga agccctcgtt ggtgtggcag cgctcgcagc cctgggaacc
     8881 gttgaggcgg ttctcgttca ccgtggcgct gtgggcgtgc gcggacaggt cgtactgggc
     8941 ggccacctga gccaggtcaa aatcctgatc gctgtggcag tcaaaacagc cggccgccgg
     9001 ctggttgacc atttccacgt cgccggtgat ttcccgatcg cagccggcga cgcacagggc
     9061 aagggcagcc agagccaaca gaagcaaggt cgtgtgtttc atggcatcct ccatgggaag
     9121 acttctctta ttatgcgcaa tcatgtgttc gctcattcat cgggccgcgg cgctcaccgc
     9181 tgggcgccga aatcgtagac gaggctgacc tggacgacgt tggccgtgta gtactggtca
     9241 tataggccgc ccagatcggc gaagtcatcg taattgtgcg ccgcgtagac cgcctccacc
     9301 gcgtagtgct tgaggaattc gtacacgccg cgcaggcgca ggctgaagct ctcgatgtcc
     9361 tgatcgagac gggtgcggta gtattggccg ccgaccccga cccggaagcc gcggcagggg
     9421 gcggtgagca gatcgccgga gaccacgtgt tcctcgaacg cgaaacagtc gccgccggcg
     9481 ttggcatagt cgccgtggta gtaggcgtac gacgccgaca cctggccgta gcggggcagg
     9541 tcggcggtca ggtcggaggc cacgcggaca aaatcaacct cggtggcgat ggttgtgtcg
     9601 gaagcggtca cgttgataat gccgcccccc tcatcgagcg agcggtcgtc caggacgcgg
     9661 tcgcgggact gatgctccac cttggcctgc caggtgaccc gggggtggcg atacttcacc
     9721 gaggcccagc cccgggtcgt ctcgtcctcg cggatgagcg ggtcgccggc ccgggtgtcg
     9781 agcatgtcgg cgccgtagcc ggcccggacc gtcagcgcgg gaaccggccg aatccatccg
     9841 gacaccacga aaccgtcggc ccggcgctcg ttgcggatgt cgtcgttgta ccggtagcgg
     9901 taaccggcgg tggcgccggc gaacgtgccg aagcgcttgt cgccgaagac ggcgtgagtg
     9961 atgttgtcgg tgctggcgag gtcatcggtg gaccgggcac ggttccacac ggcgttgtag
    10021 cggacgcccc acccgtcgcc gaacgaatag cgcccaccgc cccagacggt ggtggcaacc
    10081 agactgtcgt cgcgggtcgt cagtgaatgt tcaaagcgct gggcgccgcc gtggaggacg
    10141 agcttttcaa aaccgggcac gggtccgtag gccgtgaggc ggtagcgccg cgaggcccgg
    10201 tcggtcgccg tcagactggg accgtccgaa aagtccgagc cgcggtactc cagggtggcg
    10261 aagcggcgat tcccgccgac ggtcacgccc gcattgtaga agaagtgatc gtaatcgacc
    10321 cggtaagtcg gcagaccgag cgggggtgcg gcgaccgact tgacgaccgt cccgtgcttg
    10381 tcgacatacc ccaggccgcc gaaaagcttg agccagggga gggcctgcag ccagccgtcc
    10441 gtccgcgtgg cattccgccg ggtgaaacgg tccgccgtaa agctgtaagt ccgccggtac
    10501 tgggagttgc ggacggagag gccgaacagc cgcgccttgg tcgcgccgac caggatcgta
    10561 cggtcgttga gggtgacgtt gtcgaggtcg gcatagaggc gcaggccgtt gtcgaaggca
    10621 tagtagaaat ccttcaggga gagggccgcc ccctcgtaga ggttgtaggt cggctggaag
    10681 acgccgcggt tgccgtcctc atcggagtac acgtacccgg cgcgcagggt gccgctgtag
    10741 tcagccgcga atgcgggcgc cggcgcaagc agactcacca ggacaacgac cgccggagtc
    10801 agtatgcaca gcttcatggg catcctcc
//
LOCUS       DAOPOE010000097        10330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7369_length_10330_cov_5.482238, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000097 DAOPOE010000000
VERSION     DAOPOE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7369_length_10330_cov_5.482238"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKM94_11310"
     CDS             <1..233
                     /locus_tag="PKM94_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67263.1"
                     /translation="KRPVAVGRRNDMFIEVRDGLRGGEEICLVDPTVQEQGLPGDRAT
                     EPELNRDRTAAPSAPPAGGEGRRTGGGRRGGS"
     gene            230..1540
                     /locus_tag="PKM94_11315"
     CDS             230..1540
                     /locus_tag="PKM94_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD67264.1"
                     /translation="MNFHRIFQISFDSLAAHKLRTFLTMLGVIFGVGAVISMLSIGEG
                     AKQEALQQISILGINNIIINAKVPEQGLSSDIGLTRSRGLTLEDGRNIAEFKALVANI
                     VPQRFEPIATIYHGSAEASVRVVSTVPDYVKSSSVEVQTGRFIIDLDQAEFAQVCVLG
                     AKAKRALFAFEDPIGQSVRIGDLNFTVVGVMADKYIGRGKVEGFELKNLNEDVYIPFS
                     TAVKKLPRELEGGTEIQGGGGRMMITSTSEYQWYQTAEIDQLTVTVADLKYIEAATKL
                     VERIIERRHFGVQDYEIVVPESLLRQTEKTQRIFNIVMGAIAGISLLVGGIGIMNIML
                     ATVLERTREIGVRRAVGATRRDIMRQFLIEAVAICLVGCVVGVALGLMLSRAISFYAD
                     WPTIVSPYAIVLAVGVSTAVGVIFGLYPAGKAAKLDVIESLRYE"
     gene            1596..3089
                     /locus_tag="PKM94_11320"
     CDS             1596..3089
                     /locus_tag="PKM94_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD67265.1"
                     /translation="MSRYGMRNAALGLAAAGLMAAATPARELTYDAAMEIALRRSSRV
                     EIIRGNLEVAERKYQAEKVNFLLPEISLNGTLPGYSSAEQYTYPDGFGGTKQVGLRKS
                     LDMNTFIGLNQNLITGGSLDIRAYLQGNERHFPQTLRLETGEGVFTANESERTGRFSF
                     ALQQPLLQPSEAKFTLNNRKDELSLARLGRQEETATLKQEVAQAYIGALQLQIDKQIA
                     EEKLRSARLKADIDSVKLQDGILSEEQWLETSSARLDAELAVYDVESNLAKQMQTLAV
                     LLDMEEYQDLELAAPPVEAPLTPEEQERLLAAADRSVPIQRAEFDYAKAKRAADYSNA
                     SSGIRGTLTASYEKDAGDIETTYDGLPQTETSTLNLDTWEVKLEFSYPLWDGGSGSAE
                     SRASELEAQKAKIELERQRKIVRADIRNLTEGLDVSHRKLSILQKQVDLTAERLGIAR
                     MRFENGEISEVEYIESNVAHLDARKKYLEELQKYLVDRYTLEGKFTG"
     gene            3089..4132
                     /gene="waaF"
                     /locus_tag="PKM94_11325"
     CDS             3089..4132
                     /gene="waaF"
                     /locus_tag="PKM94_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOD67266.1"
                     /translation="MAEKIVIRTPNHLGDCIMALPMISQTREAYPGAGVTLVAPRHLA
                     ALYRPNPDIDGILELPPEHVHGLMAVMKVKDLVSPHAFDLGYILPPSFGAAAGFKLGG
                     VKERIGYIADGRRLLLTRPLALPAPLNSKHRSELYFDLLRRGAGKELPFVKPKLFLSD
                     ADVASGIELLGGFGLGADDPYVAVAFRAVAESRRWGAERYIELLKRLISRHGLRVVLV
                     GSPDDRTEGDDIAAATSTKEVINLAGKTTIREAAAVLSRAKLFVGNDSGPAHLAAAVG
                     APVVVLSGADDPKATTPLAARKKLLYLEHLPCISCERNKCPLKGAERMQCMTGLSVDL
                     VAAAAGDLLENAA"
     gene            4192..5334
                     /locus_tag="PKM94_11330"
     CDS             4192..5334
                     /locus_tag="PKM94_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD67267.1"
                     /translation="MIIDLRSVIGAAAIPADETAAAGGALRRTLDLIGLAVSDLVQPG
                     ARVFLKPDLGDPSPTTERAWSDPQLVAAVARVFTETGASVLVGDSPFVDGTPVRRRWA
                     EAGLLAPAGAEGFTLVDLEAATTRVAAVQDTVYFVPEVLVEADLVVNLARLRPHPWFG
                     AAGALTNLLGVLPGFQKIRHLVRSGADGGYGRVLADLCAVLQPGLTILQFDLEPKDAP
                     VWGILVSTDGVAVDAAVAQRTGAEEEAWPHIGPAADSGLGVGWREGIEIVGTASVPPA
                     VPETGNPRLVRQRVMRWFGGFTEPLLWTRPELDGALCDGCGECVKSCPTKALRQCTGE
                     SLPAVEARLCVGCWHCRAVCAQGAVVAGQSSLARFLTASRRRNCAF"
     gene            5483..5827
                     /locus_tag="PKM94_11335"
     CDS             5483..5827
                     /locus_tag="PKM94_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67268.1"
                     /translation="MSSRSTAGLLVLLIALGCMCGVQFVRAEGPFDVDNATGGNKTLP
                     TSTIGHPQPGDPSGDDGVTAPTRAASVVGGDWFTGFILRVSFNYAFDHWIVKPATTPR
                     QNAALPAAFRGR"
     gene            5904..8837
                     /locus_tag="PKM94_11340"
     CDS             5904..8837
                     /locus_tag="PKM94_11340"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD67269.1"
                     /translation="MKQSSPEISFDNRLLAIEEQFRQRKYSAALHDLSLLTEAAFVES
                     PPEYGLYLSLAADGSYYEGNYRKAIEQGLASARLLAGFPLNRRYGKVQLILSKAYVAV
                     GDLKNADARARDALAAYRRAEDVLGQVDALNSSARISYIRCDYHAAIESLEEAIGMID
                     DNPRKLAQVTGNLGMLLVHVGEWAKAAEALRKALQVNIDNRFEIQQAINLLSMGILAL
                     VQRQFVSSRRRFEEALDIIERHGLKREKVIALKYAGELAFEKGDTFKAKALLTNAYQS
                     GLLLSPDSALVSQAGRRLADVELALDNLDDAMKHAQKALELAMALGEKVEVALARRVI
                     AQVFAARGDFAEAVDNIRLATDLVRKVDDPYELARTLLVQADILIRVGDEDARVIRAA
                     FEEAGRLFKRLRLDYWIAETEFKTGVYACQQGDLSGGFRRLNRAEKLYLKLEENARVR
                     AVHHFLQSLSEQAVALSISQENEFKIFGNLIGPSELSDLKSSQLEDILTILLKKTGGD
                     RAVIFAPEADGETVYASLELTAFQKKRFAENFSALLGEEISRTRPTLNLDCRRDPFIN
                     ELLADIPAVVASILVVPFRMSAGQTSFLYVDRLTSDSSLNPFSQSDLNFAVGFSDLIA
                     FRWAEIEKNRLLEDNRRLKSQLMEQAAFPNIITRNHKMLDVLAQVRQVINSNISVAIE
                     GETGTGKDLVARAIHYNSNRRARRFISVNCAALPETLLESELFGYKRGAFTGADRDKP
                     GLFEEADGGTFFLDEIADMPLSIQAKILRVLECKELVRLGDSVPRGVDVRIISASNKD
                     LKEQMDRGLFRQDLYYRLSALSFRLPPLRERREDIPLLVTHFLDATGKEVDPEVMRML
                     LSYDWPGNVRELENEIKRLALLAGDRRVIGPDIVSGKLRAVAEQVESALPALQAGADV
                     EFTETYSLYDYLAEHEKQFIIRALKEKGGIKKHAAAILNIPESTLRLKIKQYNIDPKH
                     LDAVN"
     gene            9076..9753
                     /locus_tag="PKM94_11345"
     CDS             9076..9753
                     /locus_tag="PKM94_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOD67270.1"
                     /translation="MLWGFAPLDRHDWALENVLVLLVAPVLVLSYRRFPLSRVSYTLL
                     FVFLCIHEIGAHYTYAEVPYDAWFRSLFGAGLNALLGWDRNNFDRIVHFSYGLLLAYP
                     IREVFCRVAAAKGFWSYFLPLDVTMSTSMLFELFEWWAAELFGGDLGVAYLGTQGDVW
                     DAHKDMGLATTGAVIAMLVTAAINLAIQSDFAREWRESLAVKHRRPLGEVEMARLRRK
                     KQRRPSP"
     gene            9815..>10330
                     /locus_tag="PKM94_11350"
     CDS             9815..>10330
                     /locus_tag="PKM94_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67271.1"
                     /translation="MARRNAISCGMIRALCYTHPALDPFRNMAVDEWLFAQAQRRPGM
                     VVLRLYTWRTGAITFGCNQRLDHAVVHEALGGTPLIRRVTGGRALYHDPTELTYAVVV
                     NAEGLPPGPLVGSVARTSAAIAEALTAFLAELGIRSQYLRSSVGGGSHPAFFHTAPCF
                     ASAARHEVVADG"
BASE COUNT         1766 a         3298 c         3508 g         1758 t
ORIGIN      
        1 agaagcggcc ggtggcggtg ggccgccgca acgacatgtt catcgaagtg cgcgacggcc
       61 tgcggggggg cgaggagatc tgcctggtcg acccgaccgt tcaggagcag ggactgcccg
      121 gagaccgggc gaccgagccg gagctcaacc gcgaccgcac tgccgcgcca tcggccccgc
      181 cggccggggg cgaagggcgc cggacgggcg gcgggaggcg cggcggatca tgaacttcca
      241 ccgcatcttc cagatctcct tcgactcgct ggccgcccac aagctgcgga ctttcctcac
      301 catgctcggc gtcattttcg gcgtcggcgc ggtcatctcc atgctctcga tcggcgaagg
      361 ggccaagcag gaggcgctgc agcagatctc gatcctcggg atcaacaaca tcatcatcaa
      421 cgccaaagtc cccgagcagg ggctgtcctc cgatatcggt ctcacgcggt cgcgcgggct
      481 gacgctcgag gacggccgta acatcgccga gttcaaagcc ctcgtcgcca acatcgtccc
      541 gcagcggttt gagccgatcg ccacgatcta ccacgggtcg gcggaggcct cggtgcgggt
      601 ggtctccacg gtccccgact acgtgaagtc gtcgtcggtc gaggtgcaga cggggcggtt
      661 catcatcgac cttgaccagg cggagttcgc gcaggtatgc gtgctcggcg cgaaagccaa
      721 gcgggcgctg ttcgcgttcg aggacccgat cgggcagtcg gtgaggatcg gcgatttgaa
      781 tttcaccgtg gtcggggtga tggccgacaa atacatcggg cgggggaagg tggagggatt
      841 cgaactgaaa aacctcaacg aggacgtgta catcccgttc tccacggcgg tcaagaagct
      901 cccgcgcgag ctcgagggcg gcaccgagat ccagggcggc ggcgggcgca tgatgatcac
      961 ttccacctcc gagtaccagt ggtaccagac ggccgagatc gaccagctca ccgtcaccgt
     1021 tgccgacctc aagtacatcg aggcggcgac caagctcgtg gagcggatca tcgaacgccg
     1081 ccacttcggt gtccaggact acgagatcgt ggtgcccgag tcgctcctgc gccagaccga
     1141 gaagacgcag cggatattca acatcgtgat gggcgcgatc gcgggcattt cgctgctggt
     1201 cggcggcatc gggatcatga acatcatgct cgcgactgtg ctcgagcgga cgcgcgagat
     1261 cggcgtccgc cgggcggtgg gggcgacccg ccgcgacatc atgcgccagt tcctcatcga
     1321 ggccgtggcg atctgcctcg tcgggtgcgt ggtcggagtg gcgctcgggc tgatgctctc
     1381 ccgcgccatc tcgttctacg ccgactggcc cacgatcgtc tccccctacg cgatcgtgct
     1441 cgcggtgggc gtctccaccg cggtcggcgt catcttcggc ctctacccgg cgggcaaagc
     1501 agctaagctc gacgtgatcg aatcgcttcg gtacgaatag ccgccggggg agggcggacg
     1561 gcaatcggca gccacagagg agacaggatg cagatatgag cagatacgga atgcgcaacg
     1621 ccgcactggg cctggccgcg gccgggctga tggccgccgc gacgccggcg cgcgaactga
     1681 cctatgacgc ggccatggag atcgcgctgc ggcgctccag ccgcgtcgaa atcatccgcg
     1741 gcaatctcga ggtggcggag aggaagtacc aggccgagaa agtgaacttc ctcctccccg
     1801 agatctccct caacgggacg ctcccggggt actcgtcggc ggagcagtac acctaccccg
     1861 atgggttcgg gggcaccaag caggtggggc tgcggaagtc gctcgacatg aacacgttta
     1921 tcggactgaa ccagaacctg atcaccggcg ggtcgcttga tatccgggcc tacctgcagg
     1981 ggaacgaacg ccattttccc cagaccctcc ggctggaaac gggcgagggg gttttcaccg
     2041 ccaacgaatc cgagcgcacc ggccggttct cctttgccct ccagcagccg ctcctgcagc
     2101 cgtccgaagc gaaattcacg ctcaacaacc gcaaggatga gttgtcgctc gcgcgcctgg
     2161 gccggcagga ggaaacggcg acgctgaagc aggaggtggc ccaggcctac atcggggcgc
     2221 tccaactgca gatcgacaaa cagatcgccg aggagaaact ccgctcggcc cggctcaagg
     2281 cggacatcga ctccgtgaaa cttcaggacg ggattctctc tgaggagcag tggctcgaga
     2341 cctcgtcggc gcggctcgat gccgagctgg cggtgtacga cgtcgagagc aatctcgcca
     2401 aacagatgca gacgctggcg gtgctgctcg acatggagga gtaccaggac ctcgaactgg
     2461 cggcgccgcc ggtcgaggcg ccgctgaccc cggaggaaca ggagcggctg ctggcggccg
     2521 cggaccggtc ggtcccgatt cagcgggccg agtttgacta cgccaaggcg aagcgggccg
     2581 ccgactactc gaacgcctcc agcggcattc gggggacgct gacggcgagc tacgagaagg
     2641 acgccgggga catcgaaacc acgtacgacg gcttgcccca gacggagacc agcaccctca
     2701 atctcgatac ctgggaggtc aagctggagt tctcgtaccc gctctgggac ggcggctcgg
     2761 gatcggccga atcgcgcgcc tctgagcttg aggcgcagaa agccaagatc gagctcgagc
     2821 gccagcggaa aatcgttcgg gcggacatcc gcaacctcac tgagggcctc gacgtctccc
     2881 accgaaagct gtcgatcctg cagaagcagg tcgacctgac cgccgagcga ctcgggatcg
     2941 ccaggatgcg ttttgagaac ggcgagatct ccgaggtgga atacatcgag agcaacgtgg
     3001 cgcatcttga tgcgcgcaag aagtatctcg aggaactgca gaaatatctc gttgacaggt
     3061 acacgctgga aggcaagttt acgggctgat ggccgagaag atcgtcatcc gcacgcccaa
     3121 ccacctcggc gactgcatca tggctctgcc catgatcagt cagacgagag aagcctaccc
     3181 gggagccggc gtcaccctcg tggcgccgcg ccacctggcg gccctctacc ggccgaaccc
     3241 cgatatcgac ggcatccttg agctcccgcc cgagcatgtc cacggtctga tggcggtgat
     3301 gaaggtgaag gatctggtct ctccccacgc cttcgacctg ggctacatcc tgccgccctc
     3361 gttcggggct gcggcggggt tcaagctggg cggcgtcaag gagcggatcg ggtacattgc
     3421 cgacgggcgg cggctcctgc tgacccggcc gctggcgctg ccggcgccgc tcaattccaa
     3481 acaccgcagc gagctgtatt ttgacctgct ccgccgcggc gcgggcaagg agctgccgtt
     3541 tgtgaagccc aagctgtttc tgagcgacgc cgatgtggcg agcgggatcg aactgctcgg
     3601 cgggttcggc ctgggcgccg acgatcccta cgtcgcggtg gccttccggg cggtggccga
     3661 atcgcgccgc tggggcgccg agcggtacat cgagctcctc aagcgcctga tcagccggca
     3721 tgggcttcgg gtggtgctgg tgggttcgcc cgacgaccgg acggaggggg acgacattgc
     3781 cgcggcgacc tccaccaagg aagtgatcaa cctggcggga aagaccacga ttcgggaggc
     3841 ggcggctgtc ctgtcgcggg ccaagctgtt cgtgggcaac gattccggcc cggcccacct
     3901 cgccgccgcc gtcggcgcgc cggtcgtggt gctgtccggc gccgacgacc cgaaagccac
     3961 cacgcctctg gccgcccgca agaaactcct ctatctagag cacctgccgt gtatctcctg
     4021 cgaacgaaac aagtgcccgc tgaagggggc agagcggatg cagtgcatga cgggtctgtc
     4081 ggtcgatttg gtggccgcgg ccgccggcga cctgctcgaa aacgcggcct agccgccgat
     4141 ccgtcaaaac gcccctaact gcggcgatgg atcgctcgta ccatagtcgg tatgataatt
     4201 gacctccggt cggtgattgg tgcggcggcg attcccgccg atgaaacggc agcggccggc
     4261 ggggcgctcc gccggaccct cgacctcatc ggcctggccg tctccgacct cgtccagccc
     4321 ggagcccgcg tattcctcaa gcccgacctg ggggatcctt cgccgacaac cgagcgcgcc
     4381 tggagcgatc cgcagctcgt ggccgcggtc gcgcgggtgt ttaccgagac cggcgcaagc
     4441 gtgctcgtcg gggattcgcc gtttgtcgac ggcactccgg ttcggcggcg ctgggccgag
     4501 gccggattgc tggccccggc tggcgcggag ggtttcacgc tggtggattt ggaggcggcg
     4561 acgacacggg tggcggcggt gcaggacacc gtctacttcg tgcccgaggt gctggtcgag
     4621 gccgacctgg tggtaaacct cgcgcggctg cgcccgcacc cctggttcgg ggcggccggg
     4681 gccctgacca accttctcgg cgtgctgccg ggatttcaga agatccgcca cctcgtccgt
     4741 tccggcgcgg acggcgggta cgggcgggtg ctggccgatc tctgcgccgt tctccaaccc
     4801 ggcctcacca tcctccaatt cgacctcgag ccgaaggatg ccccggtctg gggaatcctg
     4861 gtctcgaccg acggggtggc ggtagatgca gcggtggcgc aacggacagg ggccgaagag
     4921 gaggcgtggc cgcacatcgg accggcggcc gattcgggcc tgggggtcgg ctggagggag
     4981 gggatagaga ttgtgggaac tgcgtcggtg ccgccggcgg ttccggaaac aggtaatccc
     5041 cgcttggtga gacaacgtgt gatgcgatgg ttcggcgggt tcacggagcc gttgctctgg
     5101 acccgacccg agcttgacgg tgcgctctgc gacgggtgcg gcgaatgtgt gaagagctgt
     5161 cctaccaagg cgttacggca gtgcaccggc gaaagcctcc cggctgtgga ggcgcgtctt
     5221 tgcgtggggt gttggcactg ccgggctgtc tgtgcgcagg gggcggtggt cgccgggcag
     5281 tcgtccttgg cgcggttcct gaccgcctcg agacgcagga actgcgcttt ttaacatatt
     5341 gcttgacaat atggtaggcg aatcttaata tgctggtggc aaattgtgcg ctttaagacc
     5401 gcacgataga ggtcggaaat attttctcct gtcgcaattt acgcgctcga caggagctga
     5461 gattcacgga ggcagtgcga tgatgagttc acgttcaaca gccggtttgc tggtcctgct
     5521 gatcgcgctc ggttgtatgt gcggcgtaca attcgtgcgg gccgagggcc cgtttgacgt
     5581 cgacaacgct accggcggca acaagacgct cccgacgagc acaattggcc atccccagcc
     5641 gggcgacccg tcgggagacg acggtgtgac ggcgccgacg cgggcggcca gtgtcgtcgg
     5701 aggagattgg ttcaccggct tcatcctgcg ggtgtcgttc aactacgctt tcgatcattg
     5761 gatcgtgaag ccggcgacga ctccgaggca gaacgcggcg ctcccggcgg ctttccgcgg
     5821 ccgctagccg caaggctcgc cgcgtcgctg ctgcgggcag cggggcggcg caacgcagac
     5881 acgaattagc gagattcgga ctgatgaagc aatcctcacc agagatctcc ttcgataatc
     5941 gcctgctggc gatcgaggag cagttccgcc agcgcaagta ctcggcggcg ctccacgatc
     6001 tgtcgctgct caccgaggcg gctttcgtcg aaagcccgcc ggagtacggg ctgtacctgt
     6061 cgctggcggc cgacggctcg tactacgagg ggaattaccg caaggcgatc gagcaggggc
     6121 tggcgtcggc ccggcttttg gccggcttcc cgctcaaccg ccgctacggc aaggtccagc
     6181 tgattttgtc gaaggcgtac gtggcggtcg gcgacctgaa gaatgccgac gcccgggcgc
     6241 gggacgcgct ggccgcctat cggcgggccg aggacgtcct cgggcaggtc gacgccctca
     6301 actcctccgc ccgcatttcc tacatccgtt gcgactacca cgcggcgatc gaatcgctcg
     6361 aagaagcgat cggcatgatc gacgacaatc cgcgcaagct cgcccaggtg acggggaacc
     6421 tcgggatgtt gctggtgcac gtgggtgagt gggccaaggc ggccgaggcc ctccggaaag
     6481 ccctgcaggt caacatcgac aaccggttcg agatccagca ggcgatcaac ctgctgtcga
     6541 tggggatcct ggcgctggtg cagcgccagt ttgtgtcgtc gcgccggcgc ttcgaagagg
     6601 cgctcgacat tatcgaacgg cacgggctca aacgcgagaa agtgatcgcc ctcaagtacg
     6661 ccggcgagct ggcgttcgag aagggggaca cgttcaaggc caaggcgctg ctgaccaacg
     6721 cctaccagtc ggggctgctg ctctcgcccg attccgccct ggtgtcgcag gcgggacggc
     6781 ggctggccga tgtcgagctg gctctggaca acctcgacga cgccatgaag cacgcccaga
     6841 aagctctcga gctggctatg gcgctggggg agaaagtcga agtagcgctg gcgcggcggg
     6901 tgattgccca ggtgttcgcc gcccgcggcg acttcgccga ggccgtcgac aacatccgcc
     6961 tggccaccga tctcgtgcgc aaggtcgatg atccctacga actcgcccgc accctcctgg
     7021 tgcaggccga cattctcatc cgggtcggcg atgaagacgc gcgggtgatc cgggccgcct
     7081 tcgaggaggc cggccgcctg ttcaagcgcc tgcgcctgga ctactggatc gccgagaccg
     7141 agttcaagac cggcgtgtac gcttgccagc agggagatct ctcgggcggg ttccgccgcc
     7201 tgaaccgggc cgagaagctc tatctgaagc ttgaggagaa tgcccgggtg cgcgccgtcc
     7261 accacttcct ccagtcgctc tcggagcagg cggtggcgct ttcgatttcc caggaaaacg
     7321 agttcaagat tttcggcaac ctcatcggtc cctccgaact gtccgacctg aagtccagcc
     7381 agctcgagga catcctgacg atcctgctga aaaagaccgg cggggaccgc gccgtgatct
     7441 tcgcgcctga ggccgacggg gagaccgtct acgcgtcgct ggagctgacg gcgtttcaga
     7501 agaagcggtt tgcggagaat ttctcggccc tgctgggaga ggagatttcg cggacgcggc
     7561 cgaccctgaa tctcgactgc cggcgcgacc cgtttatcaa cgaactgctc gccgacattc
     7621 cggccgtggt cgccagtatt ctcgtggtgc ccttccgcat gtcggcgggg cagaccagtt
     7681 tcctgtacgt cgaccggctg acctccgaca gcagcctgaa cccgttcagc cagagcgacc
     7741 tcaatttcgc cgtcggtttc agcgacctga tcgccttccg gtgggcggag attgagaaga
     7801 accgcctgct ggaggacaac cgccggctca agagccagtt gatggagcag gccgccttcc
     7861 cgaacatcat cacccgcaac cacaagatgc tcgacgtgct ggcccaggtg cgccaggtga
     7921 tcaactccaa catctcggtg gcgatcgagg gggagacggg gacggggaag gacctcgtgg
     7981 cgcgggccat tcactacaat tccaaccggc gggcgcggcg gttcatttcg gtcaactgcg
     8041 cggcgctccc ggagacgctg ctggagtcgg agcttttcgg ctacaagcgc ggggcgttca
     8101 cgggggccga ccgcgacaag cccggcctgt tcgaggaggc cgacggcggc acgtttttcc
     8161 tggacgagat cgccgacatg ccgctgtcga ttcaggccaa gatccttcgc gtgctcgagt
     8221 gcaaggagct tgtgcggctg ggcgattcgg tcccgcgcgg ggtcgacgtc cgcatcattt
     8281 cggcctccaa caaggacctc aaagagcaga tggaccgcgg gctgttccgc caggacctgt
     8341 actaccggct ctcggcgctc agtttccgcc tcccgccgct gcgcgaacgg cgcgaggaca
     8401 tccccctgct cgtgacccat ttcctcgatg cgacgggcaa ggaggtcgac ccggaggtca
     8461 tgcggatgct tctgtcgtat gactggccgg ggaacgtgcg cgaactcgag aacgagatca
     8521 agcggctggc gctgctggcg ggcgaccgcc gggtgatcgg cccggacatc gtgtcgggca
     8581 aactccgcgc cgtggccgag caggtcgaga gcgccctgcc ggcgctccag gccggcgccg
     8641 atgtcgagtt caccgagacc tactccctct acgactacct cgccgagcac gagaagcagt
     8701 tcatcatccg ggccctgaaa gagaagggcg ggatcaagaa gcatgcggcg gcgattctca
     8761 acattcccga gtcgacgctg cggctgaaga tcaagcagta caatatcgat ccgaagcacc
     8821 tggacgccgt gaactagcgt ccgggccgga ggcaagacaa gagacggccg ccccactgtc
     8881 gccggtgggg cggttttttg tcgggcgccg acccagacct tgacacgcgc ggcgcaggtt
     8941 cggtattatg gcgggcggcg cgccggctgc ctgccggcgt gtcgcagaac cgcaatccac
     9001 ggaggtgccc gcctgaatac cagagcctac acccgctatg tcttcaccct cggggcgctc
     9061 ttcgccgcct gggtcgtgct ctggggattc gccccgctcg atcgccacga ctgggctttg
     9121 gagaacgtgc tcgtcctcct cgtggcgccg gtgctcgtgt tgtcctaccg gcgtttccct
     9181 ctctcccgcg tctcctacac gctgctcttc gtatttctct gcatccatga aatcggggcg
     9241 cactacactt acgccgaagt gccgtacgac gcgtggttca ggagtctatt cggcgccggg
     9301 ctcaacgccc tgctcgggtg ggaccgcaac aacttcgacc gcatcgtgca cttctcctat
     9361 ggcctcctgc tggcctaccc gatccgggaa gttttctgcc gggtggccgc cgcgaaaggc
     9421 ttctggtcgt acttcctgcc gctggacgtg acgatgtcga cgtcgatgct gttcgagctg
     9481 ttcgagtggt gggcggccga gctgttcggc ggagacctgg gggtggcgta cctcggcacg
     9541 cagggggatg tgtgggatgc acacaaggac atggggctgg cgaccaccgg ggcggtgatc
     9601 gcgatgctgg tgacggcggc catcaatctc gccatccagt ccgactttgc gcgcgagtgg
     9661 cgggaaagcc tggcggtgaa acaccggcgg ccgcttggcg aagtcgagat ggctcgcctg
     9721 cgccgcaaga agcagcgccg cccatcgccg tgagggtcgg agcgacggtc ccgccgcggg
     9781 gtcttttgga cccagggcgc cggcgagggc ttgtttggcg cgtcgcaacg ccatatcatg
     9841 cggcatgatc cgcgcgctct gctataccca cccggccctc gatcccttcc gcaatatggc
     9901 ggtcgacgaa tggctgttcg cacaggcgca gcggcggccg gggatggtcg tcctgcggct
     9961 ctacacgtgg cgcacggggg cgatcacgtt cggctgcaac cagcggctcg accacgcggt
    10021 ggtccacgag gcgctgggcg gcacgccgct cattcgccgg gtgaccgggg gacgggcgct
    10081 ctatcatgat ccgaccgagc tcacgtacgc ggtggtggtg aacgctgagg gtcttccgcc
    10141 gggaccgctg gtgggatcgg tcgcccgcac ttcggccgcc atcgccgagg cgctcaccgc
    10201 ttttctggcg gagctgggga tccggtcgca atatctgcga tcttcggtcg gcggcggaag
    10261 tcatcccgcc ttctttcaca cggccccctg tttcgcctcg gccgcgcggc acgaggtggt
    10321 ggccgacggc
//
LOCUS       DAOPOE010000098        10197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7494_length_10197_cov_5.655886, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000098 DAOPOE010000000
VERSION     DAOPOE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_10197_cov_5.655886"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKM94_11355"
     CDS             complement(<1..639)
                     /locus_tag="PKM94_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOD67272.1"
                     /translation="MTEGDNSGRGQASGHHVGKNRIRSVDVMRGTAMVLVIVQHCYQV
                     VDPRQASAVLDALVYLPTRMASVGFMAVSGMMISFFLNTKGDPVGVQQRFARRALLLV
                     LAAHPAIHLARLFYYGPPYFASFLHHFRYDFQITDTIAVALVLAPPAIRWAGMAARGA
                     IAVGLLAAAPVALIWYQPSGPFGHMLKAVLFGGELGSGGIGFAWPLAPWVGIF"
     gene            complement(813..3515)
                     /locus_tag="PKM94_11360"
     CDS             complement(813..3515)
                     /locus_tag="PKM94_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOD67273.1"
                     /translation="MCRYLQFLTIASLTLPWGNTGLLAATIHVPGDQPTIQAGIDAAQ
                     EGDTVLVAPGRYPVNLRIANADVCLTGFGGPRLCFLEPDSGALPIIDISTGDSMETVV
                     TGFTIRKAAQNAAIRVDATSPVISGNIFTDNGGGRLGNNSVIYTTDGSAPLIQRNVFH
                     GNDSAYCVVWAGYGNPATIINNTIAGGRLGVYLYGPGAVLMNNIITACQIGVSIGAPA
                     GGYNDIWGNGVNGPLLPTDISADPHFAGASAADFSLLPGSPCVDAGIGAQAYNDPDGT
                     RNDIGAIILDQTCPTANGMALSGSDAAHVLVDAPIFAWRYYDTTLPEQNGYQVQVGTD
                     MDWTTAEQWDSGPVVSADQSCIYGGAPLADGELYFWRLRVDNGEVSSAWKEAAFQMNS
                     LPAAPTPEFPVDMEPVSSEGVYLSVVNATDAENDARVYEFEIFADPPEITPVASRAGV
                     AEGTGLTSSGVFEGLTTGSPYWWRARCFDGFENSPWSPMETFVIRAPGVIHVPGDQPT
                     IQAGIDAAQEGDTVLVAPGIYAGEGNRDLDFHGVNLVLCSDGIGQATIDCGGSPGEPH
                     RAVYLGSDEDTSSIIEGFIITGAATPGYFEGAITFHNAGGTVRNCLIIGNDCRGVAAS
                     STSPNYFRYRVLVEGCTLQNNWHGIESIANGAISHCQILYNDSVGIHLVSMDTITVTN
                     CLIVGNGQEGIFTETGRWGNYAIANNTIVLNGTGFRFYYEPPKLDAPGLAEINPSLIT
                     ANLLAANTGNGLQSDGMGLVGVLVGCNNAYGNGGEDFAVYGAQPQPGDTLGNISEDPQ
                     FCDLDTAYSVNLQSPCLPANNSCGVLIGAFGSGCGFLCGDVNGDGSGPAAPDLTYLIA
                     HLFRGGPAPAPEPADVDNDGNLDVADLTVLVDYLFRGGPPPVCD"
     gene            3804..4547
                     /locus_tag="PKM94_11365"
     CDS             3804..4547
                     /locus_tag="PKM94_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67274.1"
                     /translation="MLKKLVLTVIVLVAVGAAVAYFVRNLLVEKAVEAGSAYALGVDT
                     ELGSAALELGGGSLELNDFAVDNPEGFAEGRFLSLRRMMFDVDAGSVLDKNVVVDSFI
                     IEGVTLNLEQIDRSGNYRVLLDNINRLDLTSSEDSRTFRIALIALRDITVNGSLSLLG
                     AHSEKSFTLDDFSLRNIGGDNGATVGRLTATIVKSLITKALAAGSGVLPEGFGKNLEE
                     LKDQGVETIKTEAADRLKDLGKSLTGDRK"
     gene            complement(4594..4664)
                     /locus_tag="PKM94_11370"
     tRNA            complement(4594..4664)
                     /locus_tag="PKM94_11370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4631..4633),aa:Lys,seq:ctt)
     gene            complement(4714..4893)
                     /locus_tag="PKM94_11375"
     CDS             complement(4714..4893)
                     /locus_tag="PKM94_11375"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOD67275.1"
                     /translation="MTKTTVFFIRIDPKDKRIIERAAKIDRRTASDFVRLAALDRAEE
                     LIASKKQRASGKKGI"
     gene            complement(5421..7109)
                     /locus_tag="PKM94_11380"
     CDS             complement(5421..7109)
                     /locus_tag="PKM94_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67276.1"
                     /translation="MVSTSGGLFTYLLGSSTPIPDSLFARYDSLYLQVIFNGIILSPR
                     MPVASAGYALRVNTVDGASGGTIRLEDTRDTGLSVIGTGLHARTGVLTRFETQSAGDV
                     SSVRATIINQGSGTAYGGAFLATSAGQGPARGVYGEGRSNALMYSARGIEGYAANPGG
                     GYVYAGVFCADSLGDGDHTGVTASAFGGPGSTWTEGGSFLGSGKVGMTFGLRCGGYGE
                     SGTVVGGDFSGYCKSGSQPTYGIRSTVNADAARSGSSYAGYFDNHVPSGTNAYALYGY
                     AYDLASSASSFGVDGLTEPSGTGSSAGGYFKSDSTGTGTSYGCRGVANARGSASAYGV
                     TGVGSNQSSGAVYGGYFSAGSFGTGSKYGIYSTAPTSGYAGYFSGNVYVSGNFAVSGT
                     KSAAVEVAPEQYRLVYSQESPECWFEDFGRGQLSGGRTHIELDPLFLQTVTIDENHPM
                     NVFVQLNDPRCAGTAVICGTTGFDVVELMNGAGDASFTYRVVAKRRGYEDVRLARMTT
                     ADPGETAAQQEADRAEDQAVEQTLQEDVATHKALAESRREREREKAEPAAVGRP"
     gene            complement(7443..>10197)
                     /locus_tag="PKM94_11385"
     CDS             complement(7443..>10197)
                     /locus_tag="PKM94_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOD67277.1"
                     /translation="YEGPLDIEGVESRIDPATGELNFQAAFPASFADHPDDIYWDNSI
                     SPCVPGVDESVRALTVYDGKLIAGGGFQAAGCEIANFIASWDGSSWSPLGSGMNDIVN
                     ALTVYDGKLIAGGYFSAAGGVAANYIASWDGINWSPLGSGMNGSVYALTVYDGKLIAG
                     GVFTTAGGVAANRVASWDGSSWSPLGSGMDHWVFALTVYDGKLIAGGDFTTAGGVAAS
                     RVVSWDGSSWSPMGSGMNDDVNALTVYDGKLIAGGRFTTAGGVLVNRVASWDGSSWYP
                     LGSGMNPEVRALTVYDGKLIAGGYFTTAGGVAANNIASWDGSGWSPLGSGMNNYVYAL
                     TAYDGKLIAGGWFTTAGGVAANYIASWDGSSWSPLGSGMNDFVWALTVCDGKLIAGGA
                     FTTAGDVAANYVASWDGSSWSSLGSGMNDDVRALTVYDGKLIAGGSFATASGVAAYRV
                     ACWDGSSWSPLGSGMSSDVRALTVYDGKLIAGGYFTTAGGVAANYIASWDRSSWSPLG
                     SGMNNTVCALTVYDGKLIAGGYFTTAGGTTANYIASWDGSAWSSFGSGMNDYVWALTV
                     YDGKLIAGGDFFTAGGVAANRVASWDGSSWSPLGSGMSSWVNALTVYDGRLIAGGSFT
                     TAGGKVSAYLAAWTKGQDTDGDGVPDYADNCPSISNADQTNNDSDAYGAACDCNDNDS
                     TVYSGATELCDGQINDCSTSTLPVGEQDADGDHFIPCTVDAGGWDGDPAIIGGNDCND
                     ADPAINPLTVWYYDADGDGRGVHSDSLIQCEQPTDYVLLAPDNCPAIANPSQEDTDTD
                     GIGDACDVCPHDAADDADADGFCADVDNCPTVANPDQADSNGDGIGDACCCTLRGDFN
                     DDGSVKVSDLTALINFLFRGGASPGCLDHGDTNADGSIKVSDLTLLINYLFRGGPPPA
                     ACP"
BASE COUNT         1952 a         3364 c         3025 g         1856 t
ORIGIN      
        1 aaagatcccg acccaggggg ccagcggcca ggcaaacccg atcccccccg accccagctc
       61 cccgccaaac agcaccgcct tgagcatatg gccgaacggt cccgagggct ggtaccagat
      121 gagcgcgacc ggcgccgccg ccagcagccc gaccgcgatc gccccccgcg ccgccatccc
      181 ggcccagcgg atcgccggcg gcgccaacac cagcgcgacc gcaatggtgt cggtgatctg
      241 gaaatcgtat cgaaaatggt ggaggaagga tgcgaagtag ggggggccat aatagaacag
      301 ccgcgccaga tggatcgccg ggtgtgcggc cagcacgagc aggagcgccc ggcgcgcgaa
      361 ccgctgctgc actcccaccg ggtccccctt cgtattcagg aagaagctga tcatcatgcc
      421 ggagacggcc atgaacccca ccgacgccat ccgcgtcggc agatagacca gcgcatccag
      481 caccgccgac gcttggcggg gatccacgac ctggtagcag tgctgcacga tcaccagcac
      541 catggccgtc ccccgcatca catccaccga acggatgcgg ttcttgccga catgatggcc
      601 ggaggcttgc cctcggccgc tgttgtcgcc ctcggtcacg gcagcaccct cactgcgcga
      661 cttcgcatta tatgggtcgg actaatcctc aatgtacaag atgagggagt ttcagggaag
      721 cgcattccgc gggcgccggg cctcagggcc cgaaaattac gccgccggcc ggcccccccg
      781 gcgggccaag cgaccggccg cgagggcccc tcctaatcgc acaccggcgg gggcccgccg
      841 cgaaacaggt agtccaccag gactgtcagg tcggccacgt caagattgcc gtcattatcg
      901 acgtcggccg gctccggcgc cggggcggga ccgccccgaa acaggtgcgc gatcaggtac
      961 gtcagatccg gggccgccgg cccggacccg tcgccgttga catcgccgca gaggaatccg
     1021 cacccggacc cgaacgcccc gatcagaacc ccgcacgagt tgttcgccgg caaacagggc
     1081 gactgcaggt ttaccgagta ggctgtgtcc agatcgcaga actgcgggtc ttcggaaatg
     1141 ttgcccagcg tgtcgcccgg ctgcggctgc gcgccgtaca ccgcgaaatc ctccccgccg
     1201 ttgccgtacg cattattgca cccaaccagc acgccgacaa ggcccatgcc atcggattgg
     1261 agaccgttgc ccgtgtttgc ggccagcagg ttcgcggtta tcagcgacgg gtttatctcc
     1321 gcaagcccgg gtgcatccag cttcggcggc tcgtagtaga agcgaaaccc ggtgccgttg
     1381 aggacaatcg tgttgttcgc aatggcgtaa ttcccccaac gcccggtctc cgtgaaaatc
     1441 ccctcctgcc cgttgccgac aatcaggcag ttggtgacgg tgatggtgtc catggatacc
     1501 aggtggattc caacggaatc attatagaga atctggcaat gcgagatggc gccgttggcg
     1561 atgctctcta tgccgtgcca attgttttga agagtgcagc cctcgacgag cacgcggtat
     1621 cggaagtagt tgggcgacgt cgatgatgcc gccacgccgc ggcagtcgtt gccgatgatc
     1681 aggcagttcc gcaccgttcc gcccgcattg tggaaggtta tcgccccctc gaaatagccg
     1741 ggtgtcgcgg ccccggtgat gatgaatcct tcgatgatcg acgacgtgtc ctcatcactc
     1801 cccaggtaca ccgcccggtg cggctcgccc gggctgccgc cgcaatcgat ggtcgcctgc
     1861 cctattccgt cggaacacag cacgagattg acgccgtgaa aatcaagatc gcggttgccc
     1921 tcgcccgcat aaattccggg cgccaccagc acggtgtccc cttcctgcgc ggcatcgatc
     1981 cccgcctgga ttgtcggctg gtcccccggc acgtggatta cccccggtgc cctgatcaca
     2041 aaagtctcca tcggcgacca tggcgaattc tcgaatccgt cgaagcaccg cgcccgccac
     2101 cagtacgggg aaccggtcgt caggccctcg aagactcccg acgaggttag ccctgtccct
     2161 tcggccacac ccgcgcggct ggccaccggc gtgatctccg gcggatcagc gaagatctcg
     2221 aactcataca cccgcgcgtc gttctccgca tcggtcgcat tgaccaccga gagatagacg
     2281 ccctcgctgc tgacgggttc catgtcgacc gggaactccg gcgtcggggc cgccggcaac
     2341 gagttcatct ggaacgccgc ctctttccag gcgctcgaca cttcgccgtt gtcgacccgc
     2401 agacgccaga aatacagctc accgtcggca agcggagcgc cgccatagat gcacgattgg
     2461 tctgcagaca caaccggtcc cgaatcccac tgctctgctg tcgtccagtc catgtccgtt
     2521 cccacctgga cctggtaccc attctgctcc gggagcgtcg tgtcgtagta tcgccaggca
     2581 aagatcgggg catccacaag cacgtgggcc gcatccgatc cgctcagggc catgccgttg
     2641 gccgttggac aggtctggtc aagaataatc gcgccgatgt cgttccgagt tccatcggga
     2701 tcgttgtagg cctgagcgcc gatacccgca tcaacacacg gggaaccggg gagaagcgag
     2761 aaatccgcgg ccgacgcccc cgcgaaatgg ggatcggccg agatgtcggt aggcagcagc
     2821 gggccgttga cgccgttgcc ccagatatcg ttgtagccgc cggccggcgc tccgatcgag
     2881 acaccgatct ggcaggccgt gatgatattg ttcatcagca ccgcaccggg cccatagaga
     2941 tacacgccca gccggccccc ggcaatggta ttgttgatga tcgtcgcggg gttgccgtaa
     3001 cccgcccaga cgacgcagta cgcgctgtcg ttgccgtgaa agacattccg ctgtatgagc
     3061 ggcgccgagc cgtccgtagt atagatcacc gagttgttgc ccagccttcc gcctccgtta
     3121 tcggtgaaga tattgccgct gatgaccggc gaggttgcgt cgacacggat cgccgcgttc
     3181 tgtgccgcct tgcggatcgt gaatcccgtc accaccgtct ccatgctgtc gccggtgctg
     3241 atatcgataa tcggcaacgc accgctgtcc ggctcaagaa agcacagccg gggtccgccg
     3301 aaaccggtca gacagacgtc ggcgttggcg attctgagat tcaccggata tcggccgggc
     3361 gccaccagca cggtgtcccc ttcctgcgcg gcatcgatcc ccgcctggat ggtcggctga
     3421 tcccccggca catgaatggt cgccgccaaa agcccggtgt tgccccacgg cagcgtgagc
     3481 gaggcgattg tgagaaactg aagatacctg cacatgttat tccctcggtc aggagagtcg
     3541 gtccgatccg ccaacatata tgatacctcc aaatccggat ttgtcaacgg cctcgttcga
     3601 tccctgggcg ggagggagcc gcttacacca caaaagagtc ttctcacggt tgagtcgtgc
     3661 agggaaggaa tcgaaaacct gccgcccgat ccgcggaatg ggtccgcaat tgagcgttga
     3721 attcgctcgg cccccgccgt atcatgcggc acggtcccgg cccaactccg ggatcgaacc
     3781 taacccttgc cggaggagtc gccatgctga agaaactggt tcttacggtc atcgtcctcg
     3841 tcgccgtcgg ggcggccgtc gcatactttg tgcgcaatct gctggtcgag aaggcggttg
     3901 aagccggttc cgcatacgcc ctcggcgtgg acacggagct cggctcggct gccctcgaac
     3961 tcggcggcgg cagcctggaa ctcaacgact tcgcggtcga caatccggag ggatttgccg
     4021 agggacgctt cctgtcgctg cggcgtatga tgttcgatgt cgatgccggg tcggttttgg
     4081 acaagaacgt ggtcgtcgat tctttcatca ttgaaggcgt gaccctgaac ctcgagcaaa
     4141 tcgaccgctc gggcaactac cgggttctcc tggacaacat caaccggctg gatcttacgt
     4201 cgtccgagga ttcgcgcact ttccggattg ccctcatcgc cctgcgcgac atcaccgtca
     4261 acggctcgct cagcctgctc ggtgcgcact cggagaaatc tttcacgctt gatgacttct
     4321 ccctgcgcaa catcggcggc gacaatggcg ccactgtcgg ccggctgacg gcgacgatcg
     4381 tgaaatcgct catcaccaag gcgctggccg ccggaagcgg cgtgctcccg gagggattcg
     4441 ggaaaaatct cgaggagttg aaagaccagg gagtcgagac gatcaaaacc gaggccgccg
     4501 accggctgaa ggatctgggc aagtccctca ctggcgaccg caaatgagcg ggagagggcg
     4561 caccgacggg cggccgcgcc tcccaaagaa gaatgcgcca tcagggattc gaaccccgga
     4621 cccgctgatg aagagtcaac tgctctacca actgagctaa tagctgggtg cacaatgcct
     4681 tacatttccc agccacgttg gtttctcgta tgatcagatg ccctttttgc cactcgcacg
     4741 ctgcttcttc gatgcgatca actcctcggc acggtccaac gcagcaagcc ggacaaagtc
     4801 ggatgccgta cgccgatcaa tcttggcggc acgttcgatg atccgcttgt ccttggggtc
     4861 gatccgaatg aagaacactg tcgtcttggt cataagggcc tctgattatt ctatcgacaa
     4921 tatagccttt gcctatacag tgtcaacaga aatcgcgcaa gaaagtagaa ttgtgtctat
     4981 gcgcccaact tgtcgcatgt agagagatcg tcgccctacg ggcctccctg tgcgccacag
     5041 gggcattcag cgggctaacc ggacggcatg ccagggcgac ggggagaaga aacgagggtg
     5101 gttgtcgctg catatggatt ccttacactc gtgaaagcgc gctgatcgtg tgcttccacg
     5161 tcttgagaac acgggttcta tgttcggtgc agttcaaaca gtgaccgtct ccccgagggg
     5221 agggcccgtg gctgaaactg cagcttccgg ggggcgtcgt acgcaagtct taacagcctc
     5281 agcaaggcgc cggtcgtcgg cctgcacggg aggcgcagct aatcaaaggt gtcagttcag
     5341 gagcccgcac cggtcgatgg ccgccgtatc tgtcgtggac gagacaacga cccacgccgg
     5401 catctctgga tcgctcttct tcagggtcgc ccgactgctg ccggttcggc cttttctctt
     5461 tcccgctccc gccggctttc ggcaagcgcc ttgtgggtcg ctacgtcctc ttggagcgtc
     5521 tgttcgaccg cctgatcttc cgcgcgatcc gcctcctgct gagccgccgt ctcgccgggg
     5581 tcggcagtgg tcatgcgggc caggcgaacg tcctcataac cgcggcgctt ggcgaccacc
     5641 cgataggtga agctcgcatc gccggccccg ttcatcagtt ccaccacatc aaaccccgtg
     5701 gtgccgcaga tgacggccgt tcccgcacag cgaggatcgt tcagctgcac gaagacattc
     5761 atgggatgat tctcgtcgat ggtgaccgtc tgaaggaaca gcggatctag ttcaatatgc
     5821 gtccggccac cgctcagttg accccgtcca aagtcctcaa accagcactc cggtgactcc
     5881 tgggagtaga ccagccgata ttgctcggga gctacctcca ccgcggcgct cttggtcccg
     5941 ctgaccgcga aattgccgct gacgtagacg ttgccgctga aataaccggc gtagccgctc
     6001 gtcggagctg tgctgtagat tccatatttg gagccggtac cgaaactgcc ggcgctgaaa
     6061 tagcctccgt aaacggcccc gcttgactgg ttggagccga caccggttac gccataggcg
     6121 ctggcgcttc cccgcgcgtt ggcgacaccc cggcagccat acgaggtccc ggtaccggtg
     6181 gaatcgctct tgaagtaacc gcctgcgctg gagccggtcc ctgaaggttc ggtcagaccg
     6241 tcaacgccaa agctggaggc gctgctggcg agatcatagg catacccata gagggcatag
     6301 gcattggtgc cggagggcac atggttgtcg aagtaaccgg cgtacgagga tccgctgcgg
     6361 gccgcgtcgg cgttgaccgt gcttcggatc ccgtaggtcg gctgtgagcc gctcttgcag
     6421 taaccactga aatctccgcc aacgaccgtt ccgctctcac cataaccgcc acatcgcagg
     6481 ccgaatgtca tgccgacctt gcctgatccg aggaagctgc ccccctcggt ccatgtgctg
     6541 ccgggaccac cgaaggcact tgccgttact cccgtatggt cgccatcacc cagactgtcg
     6601 gcacagaaga cgcccgcata aacgtatcca ccgccgggat tcgccgcata tccctcgatt
     6661 cctctcgcgc tgtacatcaa ggcattgctt cttccctcgc cgtacacacc ccgagcgggc
     6721 ccctgaccgg cgctcgtcgc cagaaacgct ccgccgtagg ccgtgccgct gccctgattg
     6781 ataatcgtgg cgcgaactga cgagacatca cccgcagatt gcgtctcgaa tcgcgtgagt
     6841 acacctgtcc gagcatgcaa tccggtgccg ataacggaca gtccggtgtc cctggtgtcc
     6901 tccaatcgga tcgttccacc tgacgcccca tctacggtat tcacgcgcag ggcatagccg
     6961 gctgacgcga cgggcatccg gggcgacaga atgatgccgt tgaagatcac ctgcaagtac
     7021 agggaatcgt acctggcgaa aaggctgtcc ggaatcggcg tgctgctgcc cagaagatag
     7081 gtgaacaatc cgccgctcgt actgaccatg gggtagattt ccgaccagcg ggcggtgcct
     7141 gcggtcgggt ggtcatagag gtggaactgc gcagggtaac tgccgtcggg taccggagcg
     7201 ccggtcccgt cggtggccct gccctgatag ctgattactc gtggcgtgcc cgcagcggtc
     7261 atcgatgcga ccgccgccag agcagcgaga gtaaaacaaa cgccccgggc caagttcatg
     7321 tgacacactc cttcagaaag ccggcatgac cgactggcgt acgagcgtac gcaggtagtc
     7381 cctctgcggc cctgccggag ttgctatcgt gctctttgtt atgcgactcc acccccttcg
     7441 gctcacggac aggccgccgg cggcgggccg ccgcggaaca ggtagttgat cagcaaggtc
     7501 aagtcagaca ccttgatcga cccgtccgca ttcgtgtccc cgtggtcaag gcagcccggt
     7561 gaggccccgc cccggaacag gaagttgatc agggccgtga gatcggagac cttcacggag
     7621 ccgtcgtcgt tgaagtcgcc acgaagagtg caacagcaag catcgccgat gccgtcgcca
     7681 ttgctgtccg cctgatcggg attcgccacg gtcgggcagt tgtccacgtc ggcgcagaac
     7741 ccgtctgcat cggcatcgtc ggccgcatca tgagggcaga catcgcaagc gtcgccaatg
     7801 ccatccgtgt cggtatcctc ttggctggga ttcgcaattg ccgggcaatt atccggcgcg
     7861 agcaggacat aatccgtggg ttgttcgcat tggatgagtg aatcactatg cacacctcgg
     7921 ccgtcaccat cggcgtcgta gtaccaaacg gtgagtgggt taatggcggg gtcggcatcg
     7981 ttgcagtcgt tgccgccgat aatcgccgga tccccatccc agccaccggc gtcaacggtg
     8041 caagggatga agtggtcacc gtcggcatcc tgttcgccaa ctgggagggt cgaagtgctg
     8101 caatcattga tttgtccgtc acaaagttcg gtggcgccgg agtagacggt gctgtcattg
     8161 tcattgcagt cacaggcggc gccataggcg tcactgtcat tattcgtttg gtcagcattt
     8221 gaaatagagg ggcagttgtc agcgtagtcg ggaacgccgt caccgtcagt gtcctggccc
     8281 tttgtccacg cggcgagata cgccgaaacc tttccgcccg cggttgtgaa cgagccgcca
     8341 gcgatcaggc gaccatcgta gacggtcagg gcattgaccc agctactcat ccccgacccc
     8401 aggggggacc aactgctccc atcccaggag gctactctgt tcgccgccac gccgccggcc
     8461 gtgaagaaat cgccccccgc gatcagcttg ccgtcgtaca cggtcagggc ccagacatag
     8521 tcattcatgc ccgaaccgaa ggaggaccac gccgaaccgt cccaggaggc aatgtagttg
     8581 gcggtggtcc cgcccgccgt ggtgaaatat ccccccgcga tcagcttgcc gtcgtagacg
     8641 gtcagggcac agacagtgtt attcatcccc gaccccaggg gggaccaact gctcctatcc
     8701 caggaggcta tgtagttcgc cgctacgcca ccggccgtgg tgaaatagcc ccccgcgatc
     8761 agcttgccgt cgtagacggt cagggcacgg acatcgctac tcatccccga ccccaggggg
     8821 gaccaactgc tcccatccca gcaggctact ctgtacgccg ccacgccgct ggccgtggcg
     8881 aaagagcccc ccgcgatcag cttgccgtcg tacacagtca gggcacggac atcgtcattc
     8941 atccccgacc ccagggacga ccaactgctc ccatcccagg aggcaacgta gttcgccgcc
     9001 acgtcgccgg ccgtggtgaa agcgcccccc gcgatcagct tgccgtcgca gacggtcagg
     9061 gcccagacaa agtcattcat ccccgacccc aggggggacc aactgctccc atcccaggag
     9121 gctatgtagt tcgccgccac gccgccggcc gtggtgaacc agccccccgc tatcagcttg
     9181 ccatcgtagg cggtcagggc atagacatag ttgttcatcc ccgaccccag gggggaccaa
     9241 ccgctcccat cccaggaggc tatgttgttc gccgccacgc cgccggccgt ggtgaaatag
     9301 ccccccgcga tcagcttgcc gtcgtacacg gtcagggccc ggacttcggg gttcatcccc
     9361 gaccccaggg ggtaccaact gctcccatcc caggaggcta ctctgttcac cagtacgccg
     9421 ccggccgtgg tgaatcggcc ccccgcgatc agtttgccgt cgtagacggt cagggcattg
     9481 acatcgtcgt tcatccccga ccccatgggg gaccaactgc tcccatccca ggagactact
     9541 ctgctcgccg ccacgccgcc ggccgtggtg aaatcgcccc ccgcgatcag cttgccgtcg
     9601 taaacggtca gggcaaagac ccagtgatcc atccccgacc ccagggggga ccaactgctc
     9661 ccatcccagg aggctactct gttcgccgcc acgccgccgg ccgtcgtgaa aacgcccccc
     9721 gcgatcagct taccgtcgta gacggtcagg gcatagacag agccattcat ccccgacccc
     9781 aggggggacc agttgatccc atcccaggag gctatgtagt tcgccgccac gccgccggcc
     9841 gcggagaaat agccccccgc gatcagcttg ccgtcgtaga cggtcagggc attgacaatg
     9901 tcattcatcc ccgaccccag gggggaccaa ctactcccgt cccaggaggc tatgaagttg
     9961 gctatctcac aaccagccgc ctggaaacca ccccccgcga tcagcttgcc gtcgtagacg
    10021 gtgagggcac ggacactctc atctacgccg ggaacgcatg gtgatatgct attatcccag
    10081 tagatgtcat cggggtggtc ggcgaaagaa gcaggaaagg cagcttggaa gttcagctca
    10141 ccggtggcgg ggtcgatccg tgactcaacg ccttcgatgt caagcgggcc ttcgtag
//
LOCUS       DAOPOE010000099        10062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7608_length_10062_cov_5.710103, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000099 DAOPOE010000000
VERSION     DAOPOE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7608_length_10062_cov_5.710103"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKM94_11390"
     CDS             <1..470
                     /locus_tag="PKM94_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67278.1"
                     /translation="DRAEHIHLLGESLAADGNLAAAEQRFREAIRKRPDYAPALRSLA
                     QSRMRSGSFGEACRLLRQARRLDPGAAAILEDLGAAYLRLNYLDSAAAVADTLLAMES
                     RSPGGHLIRMLAAVRSGDEATARRHFLTYREHGRQRPEYAQIVSAYGGLAPDR"
     gene            590..2641
                     /locus_tag="PKM94_11395"
     CDS             590..2641
                     /locus_tag="PKM94_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD67279.1"
                     /translation="MTDRPQPRRHTNRLIEENSPYLLQHAHNPVDWHPWGEEALARAK
                     AEDKPIFLSVGYAACHWCHVMERESFEDEETARLLNDHFVSIKVDREQRPDIDQIYMS
                     FTMAMTGGGGWPMSVFLTPDLKPFFAGTYFPPEDTQGRPGFKHVLREIAAAYRESKAQ
                     LLESSEAIFGQIAGYLRSSPEPTLLTPELVHKVARTLMGAYDSRFGGFGHAPKFPHGV
                     ELSFLLRQYKATGDLSLRAAVEKTLGAMAEGGIYDHLGGGFARYSTDERWLVPHFERM
                     LYDNALLVPVYSEAYRITRDRRCRTVVVETLDWVLREMTAPCGGFYSAVDADSEGEEG
                     KFYLWTKEEIDRVLGDRAELFCRYYNVTAAGNFEGKNILHLSAESEGVRRESGGEFEA
                     FLAGCRRDLSEVRSRRPRPQVDDKILTSWNGLMISALCHGYQITEEVRFLAAAVQAAA
                     FIRDELFHRGSLAHAYRDGRRSEGEFLEDYAYLVHGLLDLSETDTAGARGWLEFADRL
                     AGRAIGLFGDDQGRFFLRPADAGGLILRPREETDASLPAPGSILMHALLKLNRLTGNR
                     AYLQTAQQALRAVSGLLDKYPSGMAAAAIALQYYAGDKIEIVIVGRGRTRDDMLRAVY
                     DHFLPNRVVALSDTGAEPLPLFEGRGVANGETRAYLCRNSVCRLPVTTAADLAAQLAA
                     L"
     gene            2822..3283
                     /locus_tag="PKM94_11400"
     CDS             2822..3283
                     /locus_tag="PKM94_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOD67280.1"
                     /translation="MPSTMTTRMHWKGGMQFGATGQFGHAITTDISLAGGGQEGGYKP
                     TELLFWGMAACTGVDVVRILQKQRQELAGLEIEITAEQPDDYPKPFQNIQIRYIFRGR
                     NLDPKKLRQAVELSESKYCSVGQTIRRETAITSHIEIIASQPEAAAADGNS"
     gene            3351..3764
                     /locus_tag="PKM94_11405"
     CDS             3351..3764
                     /locus_tag="PKM94_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOD67281.1"
                     /translation="MPHHTSPPTATAFALRRESTKPFAEVVGNLERRTAEHNFRVLHV
                     HDVQATLAERGLARGPLKIIEVCNAGFAHQALGKDINVALFMPCRFVVHTEGGTTVVT
                     LARPSMIADLMPHAGLTELARGIEQTLTAVVEESL"
     gene            3996..4181
                     /locus_tag="PKM94_11410"
     CDS             3996..4181
                     /locus_tag="PKM94_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD67282.1"
                     /translation="MPLYEYKCLDCGKDFEELVSADQEEVSCPACGSQEASRRLSTFA
                     WGSVAASCATPSGSGFR"
     gene            4459..4962
                     /locus_tag="PKM94_11415"
     CDS             4459..4962
                     /locus_tag="PKM94_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD67283.1"
                     /translation="MKGRDRMTPVNQETLQALTSGIQAEVASYVFYREALKKQQVTQI
                     RTILEELALEEKRHFHVLERQYDSLVRSEKWISTADLMKQEGLPEVPEEMSRTHTALM
                     GEVAQAASLREILAIALRLEEQARDIYARALTFVQSEEGRKVFERLVSFEEGHVRKVK
                     GMMEKYL"
     gene            4994..5788
                     /gene="kdsA"
                     /locus_tag="PKM94_11420"
     CDS             4994..5788
                     /gene="kdsA"
                     /locus_tag="PKM94_11420"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOD67284.1"
                     /translation="MIEAALSRVAAGEFFLIAGPCVVESHALCLEIAERVAELCARRR
                     VPYIFKASFKKANRTAPGSYTGPGIAEGLAALAAVRERMKLPVLTDVHETSEVAAVAA
                     VVDILQIPAFLSRQTDLLAAAGATGKWVNIKKGQFLAPDDMAYAVQKTGSDKVMLTER
                     GTTFGYHNLVVDFRSLLIMREYGRTVFDATHSLQLPSAASGVSGGRPDMVIPMAKAAV
                     AIGVNGLFVETHPDPARARSDAGAMLPLDRIPDLLDQVLRIRNAAG"
     gene            5792..6328
                     /locus_tag="PKM94_11425"
     CDS             5792..6328
                     /locus_tag="PKM94_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOD67285.1"
                     /translation="MPKPKLTRTQLVGRFKNVKLLAMDVDGVLTDDTLYFGPDGFEMK
                     RFHISDGLMMVLAMRAGLQLAVVSGRYSAATETRMRDLGIPHVLQGMKDKVAQITPLL
                     AALQLSFADIAFVGNEILDITLAERAGLALAVADASPHLVEVVDYVTAARGGAGAVRE
                     ILEAYFEAIGQEPRRYLV"
     gene            6325..7293
                     /locus_tag="PKM94_11430"
     CDS             6325..7293
                     /locus_tag="PKM94_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOD67286.1"
                     /translation="MTNDLRAYAAQVIRMEAEAVAAMAEHLDDNFSRAVEAILDCRGR
                     VIVSGMGKSGLVGKKIAATFNSTGIASIFLHPAEAMHGDLGLMQADDLLMIISKSGMV
                     GESELVLSVAKRKGLPIIYLAGTLDSEMARRADIVLDCSVDKEACPNNLVPTSSSTAA
                     LVMGDALAVALLKARHFTAEDFAALHPGGFIGLRLLKRVSELHHTGAELPLVGPEATM
                     KEMILEMTGKRLGCVLMTDETGQVAGIFTDGDLRRLAEQGSNLYEVTARSAMIKNPKR
                     IAADALIDAALALMERYAITQLPTVDADGKLAGVIHLHDILKSKLI"
     gene            7297..8214
                     /locus_tag="PKM94_11435"
     CDS             7297..8214
                     /locus_tag="PKM94_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67287.1"
                     /translation="MRLSQRIRRGAVHRLATAALAVLNRIPRRAALAIGAWFGLAAWG
                     LLPRHRHRSIATLNLVYGDRLSRKEAAAIARRFFVNSGKNLADFVRFRDHFREEIAPR
                     VTIEGLEHYLGAFAGGGGVFGVTGHIGHFEILAARLAMEPHPVAVIARELYDPRLNRL
                     LVQARESVGLKNVLTTDSPRVLLEWLKRNGAVGALIDTDSFRVRSEFIDWFGRPARTP
                     IGQALLGLRAGSAFVPMACLRRPDDGYHIIIKQQVRVEETGDAETDARRITEACVREL
                     QTIIDSHRDQWIWLHDRWRTRPPTTSESP"
     gene            8298..8891
                     /gene="lptC"
                     /locus_tag="PKM94_11440"
     CDS             8298..8891
                     /gene="lptC"
                     /locus_tag="PKM94_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOD67288.1"
                     /translation="MHQPKVLALILTLLLAIALSGCGRDDLPSNGRTATVDSAIHPDT
                     ETSGAVIHLFDRGQVTAEIHAAKIVQFSSKDSTMAYALDIDSYDSAGTVGTEVTGDSA
                     IIRQDEGFFTVFGNVVVITPDQRRLETEYLHWNSQSDRIYTDAYVTITTPQETIRGWG
                     LEADQRVRRYKILHQVSAEIEDASRLEQEVGPGGSGE"
     gene            9049..9465
                     /locus_tag="PKM94_11445"
     CDS             9049..9465
                     /locus_tag="PKM94_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOD67289.1"
                     /translation="MNRYFEFSRRQLYVLAVLCTSAAALGSYAALARLDFSAPEPAGL
                     PLTAGTFADPALPALSIGGMAIGSFVLDPNTAPSDSLELLPGIGPTKAAAMIAARDTL
                     PFAGPDDLLRVDGIGPKTLQKVRPFIKIAGPETPAP"
     gene            9462..>10062
                     /locus_tag="PKM94_11450"
     CDS             9462..>10062
                     /locus_tag="PKM94_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67290.1"
                     /translation="MNTPSFLVGLDLQPDSCRAVRMRVDTPRPEVAAVAEITPGRPAP
                     EEFFAEAALVAAVPGQYAIAKNIPLTDGGSDPCARALFELAQSLTGSPTDYWLDAAPT
                     GLAGLMLGMAVRRSVLRQELLDPLAGMTGRTPIRAEVRGLALAHGYLAFCRPSRGGVL
                     VMIDQDGGHGSAAVLYRNRVVALAHVPTGHLDLSAAADRE"
BASE COUNT         1747 a         3344 c         3249 g         1722 t
ORIGIN      
        1 ccgaccgggc ggagcacata catctgctcg gcgaatcgct cgcggccgat gggaacctgg
       61 ccgccgccga gcagcggttc cgcgaggcga tccgcaaacg gccggactac gccccggcgc
      121 tgcgcagtct ggcgcagtcg cgcatgcggt ccgggtcttt cggcgaagcc tgtcggctgc
      181 tccggcaggc ccgccgcctc gatcccggag ccgccgccat cctcgaggat ctcggcgccg
      241 cctaccttcg tctcaattat cttgactccg cggccgccgt ggccgacact ctgctggcga
      301 tggagagccg ctcccccggc ggacatctga tccgcatgct ggccgcggtg cggtcgggcg
      361 acgaggccac cgcccgccgg cacttcctga cctatcgcga acacggccgg cagcggccgg
      421 agtacgcgca gattgtgtcc gcctacggcg gcctggcccc agatcgctga ggcgcacggc
      481 ggaacagccg ggggactgcg gctgttgagc cagtaccgcc ggcgcgcaca gggcgccgcg
      541 cggtggtgaa cgctcctcgg ccgacctgtc ggagaaaggt agaacgtccg tgactgaccg
      601 cccccaaccc cgccggcaca ccaaccgcct gatcgaggag aattcccctt acctgctgca
      661 gcacgcgcac aacccggtcg actggcaccc ctggggggag gaggctctgg ccagggcgaa
      721 agcggaggac aagccgattt tcctctcggt cggctacgcc gcctgccact ggtgccacgt
      781 catggaacgc gagtcgttcg aggacgagga gaccgcccgg ctgctcaacg accatttcgt
      841 gagcatcaag gttgaccggg agcagcgccc ggatatcgac cagatctaca tgtcgttcac
      901 gatggcgatg acgggcggcg gggggtggcc gatgtcggtt tttttgacgc ccgatctcaa
      961 gccttttttc gcgggcacgt attttccgcc ggaggacact caggggcggc ccgggttcaa
     1021 acatgtcctc cgggaaatcg ccgccgccta ccgcgagagc aaggcgcagc tgctggagtc
     1081 ctcggaggcg attttcggcc agatcgcggg ctacctcagg tcatcgcccg agccgacgct
     1141 cctgacgccg gagctggtcc acaaagtagc ccgcacgctc atgggggcgt acgacagccg
     1201 tttcggcggc ttcggacacg ccccgaaatt cccccacggc gtggaactgt cattcctgct
     1261 gcgccagtac aaggccacgg gcgacctgag tctccgcgcc gcggtcgaga agacgctggg
     1321 cgccatggcc gaggggggca tctacgacca cctcggcggc gggttcgccc gctactccac
     1381 cgacgaacgc tggctggtgc cccattttga gagaatgctc tacgacaacg ccctcctcgt
     1441 ccccgtctac agcgaggcct accggatcac ccgcgaccgt cgctgccgca cggtcgtagt
     1501 cgagacgctc gactgggtcc tgcgcgaaat gaccgccccc tgcggagggt tctactcggc
     1561 cgtcgacgcc gacagcgagg gggaggaggg gaaattctac ctatggacca aagaagaaat
     1621 cgaccgtgtc ctgggggacc gcgcggagct tttctgccgc tactacaatg tcacggctgc
     1681 ggggaatttc gaggggaaga atatcctcca cctctccgcc gaatccgagg gagtgcgccg
     1741 ggagagcggg ggggaattcg aggccttcct cgccggctgc cggcgcgacc tgagtgaggt
     1801 ccgcagccgc cgcccccggc cgcaggtcga tgacaagatc ctcacctcgt ggaacggcct
     1861 gatgatcagc gctctgtgtc acggctacca aatcacggaa gaagtccggt ttctcgcggc
     1921 ggcggtccaa gccgcggcgt ttatccgcga cgagctgttc caccgcggct cgctcgcgca
     1981 cgcgtaccgc gacggccggc gcagcgaggg ggagttcctc gaagactatg cgtaccttgt
     2041 ccacggcctg ctcgacctat ccgagaccga cacggcgggc gcccgcggct ggcttgaatt
     2101 cgccgaccga ctcgcggggc gcgccatcgg actgttcggc gacgaccagg gacggttctt
     2161 tctccgcccg gccgatgccg gcggcctgat tctgcgcccc cgcgaggaga ccgacgcctc
     2221 cctcccggcg cccggctcga tactcatgca cgcccttctc aagctgaacc ggctgaccgg
     2281 caaccgcgcg tatctgcaga ccgcccagca ggcgttgcgg gcggtgagcg ggctcctcga
     2341 caaatatccc tccgggatgg ccgcggcggc gatcgcgctg cagtattacg ccggcgacaa
     2401 gattgagatt gtcattgtcg gccggggccg cacgcgcgac gacatgctgc gggcggtcta
     2461 cgaccacttc ctccccaacc gggtggtcgc cctcagcgac accggcgccg aaccgctccc
     2521 cctgttcgag gggcggggcg tggctaacgg cgagacccgc gcgtatctct gccgcaattc
     2581 cgtctgccgc ctcccggtca cgacggcggc ggatctggcc gcgcagcttg ccgccctcta
     2641 gccccctttc ccgcggccgc tctcaaccgg ccgcgggccc ggtcggtgac ggggcgattc
     2701 tgcccgagcg atcccgtccg cccgcttctc cccgcgactc gcaggaatca ttccgctttg
     2761 tggatcatcc aagatatgat atactgaaca gagacataat tgccggcaaa ggagacgcgc
     2821 catgccatcg accatgacca cccggatgca ctggaaaggc gggatgcagt tcggggccac
     2881 cggccagttc ggccacgcca tcaccaccga catctccctg gccggcggcg gacaggaagg
     2941 cggctacaaa ccgaccgagc tgttgttctg ggggatggcg gcctgcaccg gggtcgacgt
     3001 tgtgcgcatc ctccaaaaac agcgccagga actggccggt ctggagatcg agatcaccgc
     3061 cgagcagccc gatgattacc ccaagccgtt tcaaaatata cagatacgct acattttccg
     3121 gggccggaac ctcgacccga aaaagctccg ccaggcggtc gagctgtcgg aatccaaata
     3181 ctgctcggta ggccagacca ttcgccgcga gaccgccatc acctcccaca tcgaaatcat
     3241 cgcgtcccag ccggaggcgg cggccgccga cggcaattcc tgaatcgttt ccgcggctcg
     3301 cccatctaac cggaaaagaa gacgagtctg aagacgaaca ggagtattct atgccgcacc
     3361 atacctcgcc gccgaccgcc accgccttcg cgctgcgccg ggaatcgact aaaccgtttg
     3421 ccgaggtagt cggcaacctc gagcgccgga cggcggagca caatttccgg gttctgcacg
     3481 tgcacgatgt gcaggcgacg ctggcggaga ggggactcgc gcgcggacca ctcaagatca
     3541 ttgaggtgtg caacgccggt ttcgcccatc aggcgctcgg caaggatata aacgtcgccc
     3601 tcttcatgcc ctgccgtttc gtggttcaca ccgagggggg cacgaccgtc gtgaccctcg
     3661 cgcggccgtc aatgatcgcg gacctgatgc cgcacgccgg cctgaccgaa ctggccaggg
     3721 ggatcgagca gacgctgaca gccgtggtgg aggagtcgct ttagccgggc acattccgcg
     3781 cggcgggtca cggagtataa tgatgaaatg gcaacgcctt ctgaaagtga aaccggcgct
     3841 ctggacgctc gccggcggcg ccgtcggctt cgccgtctac ctgctctacg ccggcctcgg
     3901 ctcgacctga cccctcatgt gcaacccgta tgtcacgacg acagcgggtg cggtgaccgg
     3961 ggcggtcatg tcggtaatgg aatgaggtaa cgcatatgcc gttgtacgaa tacaaatgcc
     4021 ttgactgcgg aaaggacttc gaagaactcg tgagcgccga ccaggaggaa gtgtcctgtc
     4081 cggcgtgcgg ttcgcaggaa gcctcccgta gactctccac gtttgcctgg ggcagcgtcg
     4141 ccgcctcgtg cgccaccccg tccggcagcg gcttccggtg agggtgatca cgcgttccac
     4201 ggtcgtggaa cgggaaagcg caacttaacg cgctgaaagt acgatgcgga tccggcggcg
     4261 atcccgccgc gaggctgcgc cgaaaataat ccccctcgtc ctctcacgcc cgcacggccg
     4321 ggcgctttca ccagccccgt gtttcgtttt agggggtccg cgcacagccc gcccggcctg
     4381 tcgctgcgtg ttgagccccg ccttgacaac gatgaccaaa taggtgattt tgcggccaaa
     4441 tggtggtgtt ccgaattggt gaaaggacgc gatcgcatga ccccggtcaa ccaggaaacg
     4501 cttcaggcgc tgacctccgg catacaggcg gaggtcgcca gttacgtgtt ttaccgcgaa
     4561 gccctcaaga agcaacaggt tacgcagatc agaacgattc ttgaagaact cgcgctggaa
     4621 gagaagcgcc attttcacgt gctcgaacgc caatacgact ccctcgtccg cagtgagaaa
     4681 tggatcagca ccgcggatct catgaaacag gaggggctgc cggaggtccc ggaggagatg
     4741 agccggacac acacggcgct catgggcgag gtggcccaag ccgcatcgct gcgggaaatt
     4801 ctcgccatcg cgctgcggct tgaggagcag gcccgcgata tctatgcccg ggcgttgact
     4861 ttcgtgcaga gcgaagaggg ccggaaagtg ttcgagcgcc tggtgtcgtt cgaggaaggg
     4921 catgtccgga aggtcaaggg gatgatggag aagtacttgt gaccggacgg ccggacggct
     4981 tatattgcct gccatgatcg aggccgcact ctcccgggtc gccgccggcg agtttttcct
     5041 gatcgccggt ccctgcgtcg ttgaatcaca cgcgctctgc ctcgaaatcg cggagcgcgt
     5101 ggcggagttg tgcgcccgcc gccgtgtccc ctacattttc aaggcctctt tcaagaaggc
     5161 caaccgcacc gcgccgggtt cctacaccgg tccgggaatc gcggaggggc tggccgccct
     5221 cgcagccgtc cgcgagcgta tgaaactgcc ggtgctgacc gatgtccacg agacctccga
     5281 ggtcgccgcc gtggccgcag tcgtcgatat cctgcagatc ccggcgtttc tctcccggca
     5341 gaccgacctt ctcgccgcgg ccggggccac cggcaagtgg gtgaatatca agaaggggca
     5401 gtttcttgcc cccgacgaca tggcctatgc ggtgcagaag accggttccg acaaagtgat
     5461 gctcaccgag cgggggacaa ccttcggcta ccacaacctc gtggttgatt tccgctcgct
     5521 cttgatcatg cgggagtatg gccggacggt gtttgacgcc acgcactcgc tgcagttgcc
     5581 gagcgccgcc agcggggtgt cgggcgggcg gccggacatg gtgatcccga tggcgaaagc
     5641 tgcggtcgca atcggcgtca acggcctgtt tgtggagacc caccccgatc cggcccgggc
     5701 ccggtccgac gccggcgcca tgctccccct cgaccgcatc cccgatctcc tggatcaggt
     5761 ccttcgcatc cggaatgccg caggataagc catgcccaag cccaaactca cccgcacaca
     5821 actggtcgga cgcttcaaga acgtaaagct gctggcgatg gatgtcgacg gcgtcctgac
     5881 ggatgacacg ctgtacttcg gccccgacgg cttcgagatg aagcggtttc acatttcgga
     5941 cgggctcatg atggtactgg cgatgcgggc cggtctgcag cttgcggtgg tctcggggcg
     6001 ctactcggcg gcaaccgaga cgcggatgcg cgacctcggc atcccgcacg tcctgcaggg
     6061 gatgaaggac aaagtggcgc agatcactcc cctgctggcc gcgctgcagc tctcgtttgc
     6121 ggatatcgct tttgtcggca acgaaattct ggacatcacg ctggccgaac gcgccggtct
     6181 cgcgctcgcg gtcgccgacg cctccccgca tctggtcgag gtggtcgact acgtgaccgc
     6241 cgcccgcggg ggggcggggg cggtgcggga gattctggag gcttacttcg aagcgatcgg
     6301 gcaggagccc cggaggtacc tggtatgacc aacgacctgc gcgcctatgc cgcccaggtg
     6361 atcaggatgg aggctgaggc ggtagcggcc atggccgagc acctcgacga caatttcagc
     6421 cgggcggtgg aggccattct ggactgccgc ggccgcgtca tcgtctccgg catgggaaaa
     6481 tccggcctgg tcggcaagaa gatcgccgcg accttcaact ccaccggcat cgcctccatc
     6541 ttcctccacc cggccgaggc catgcacggc gacctcgggc tcatgcaggc ggacgatctg
     6601 ctgatgatca tctccaagtc ggggatggtc ggcgagtcgg agctggtgct gtcggtcgcc
     6661 aagcgcaagg gactgccgat catctacctg gccggcacgc tcgattcgga gatggcccga
     6721 cgcgccgata tcgtactcga ctgcagcgtg gacaaagagg cctgccccaa caacctcgtg
     6781 cccacttcct cgtcgaccgc ggcgctggtg atgggggacg cgctcgcggt ggcgctcctg
     6841 aaagcccgcc acttcacggc cgaggatttc gccgccctcc atccgggcgg gtttatcggc
     6901 ctgcgcctgc tcaaacgagt gagcgaactg catcacaccg gcgccgagct ccccctggtc
     6961 gggccggagg cgacgatgaa ggagatgatc ctcgagatga ccggcaagcg gctcgggtgc
     7021 gtgctcatga ccgacgaaac gggacaggtg gcggggatat ttaccgacgg cgacctgcgg
     7081 cggctggcag aacaggggag caatctctac gaggtgacgg cccgttcggc gatgatcaag
     7141 aatcccaagc ggattgcggc cgacgcgctc atcgatgccg cgctcgccct catggagcgc
     7201 tatgcgatca cccagttgcc gacggtggac gccgacggga agctggcggg cgtgatccat
     7261 ctccacgaca ttctcaagtc caagctcatc tgagtcatgc ggctgtccca acggatacgg
     7321 cgcggggcgg tgcaccgcct ggccaccgcc gctctggcgg tgctcaaccg cattccgcgc
     7381 cgggccgccc tggcgatcgg ggcctggttc ggtctggccg cctggggcct tctcccgcgg
     7441 caccgccacc gcagtatcgc caccctcaat ctcgtgtacg gcgaccgcct aagccggaaa
     7501 gaagcggccg cgatcgcccg gagattcttc gtaaatagcg gcaagaacct ggcggacttt
     7561 gtgcgattcc gcgatcattt tcgggaggag atcgcccccc gcgtcacgat cgaaggcctg
     7621 gagcactacc ttggcgcgtt cgccggaggg ggcggggtgt tcggcgtgac cggccacatc
     7681 ggtcatttcg agatcctcgc ggcgcgtctg gccatggaac cccacccggt cgccgtgatc
     7741 gcgcgcgagc tgtacgatcc ccgcctgaac cggttgctgg tgcaggcccg cgagtcggtc
     7801 ggcctgaaga atgtcctcac caccgattcc ccccgcgtgc tgctcgagtg gctcaagcga
     7861 aacggggcgg tgggcgcgct gatcgacacc gactcgttcc gtgtccgcag cgaatttatc
     7921 gattggtttg gccgtccggc ccggaccccg atcgggcagg cgctgctggg tctgcgcgcc
     7981 gggtcggctt ttgtcccgat ggcctgtctc cggcgtcccg acgacggata tcatatcatt
     8041 ataaaacaac aggttagagt cgaagagacg ggtgacgccg agactgacgc ccgcagaatc
     8101 accgaagctt gtgtccgcga gctacagaca attattgaca gccaccgcga tcagtggatc
     8161 tggctgcacg accgctggcg gacccgcccg ccgaccacct cagaatcccc ttgacaaatc
     8221 cccgattata tcgtctttac ctgccgacat taggagaact gaggaagtag aacataagaa
     8281 tgctcattat taatagtatg caccaaccca aggttcttgc gctgatcctc accctccttc
     8341 tcgcgatcgc cctgagcggt tgcgggcggg acgacctgcc ctccaacggg cgcaccgcca
     8401 ccgtggacag cgcgatacat ccggacaccg aaaccagcgg cgccgtcatc cacctgtttg
     8461 atcgcggcca ggtgacggcc gagattcacg cagccaagat cgtgcagttt tcctccaagg
     8521 attcgacgat ggcgtacgcc ctggatatcg actcttatga ttcggccggg acggtcggta
     8581 ccgaggttac gggagattcc gcgattattc ggcaggatga gggatttttc acggtcttcg
     8641 gtaacgtcgt ggtcatcaca cccgaccagc gccgcctcga gactgagtat ctccactgga
     8701 actcccagag cgaccggatc tacaccgacg cttatgtgac gatcaccact ccgcaggaga
     8761 cgatccgcgg atggggtctg gaggcggacc aacgcgtacg ccgctacaag attctccatc
     8821 aggtgtcggc cgagatcgaa gacgcctccc gactggagca ggaggtcggc ccggggggat
     8881 cgggcgagta gtgcaacggt tctacagcgc gcagatattg gggtcggcgg tcgccggccc
     8941 cgtttttgtt cggccttccg agccgtgggg ggcgcaagcg tgcccgcctc ttgcgcactc
     9001 ccccgcgccg tggtatactt cgggacaaac gcgagtcggg gactgttcat gaaccggtat
     9061 ttcgagttct ccagacgtca gttgtatgtt ctcgccgtgc tctgtacctc ggcggcggct
     9121 ctgggcagtt atgccgcgct ggcgcgtctg gacttctcgg cgccggaacc ggcgggcctg
     9181 cccctgaccg ccgggacctt cgccgacccc gccctcccgg ccttatcgat cggggggatg
     9241 gcgatcggca gtttcgtgct cgacccgaac acagccccct ccgactcgtt ggagttgctg
     9301 ccggggatcg ggccgaccaa agcggcggcc atgatcgccg ctcgcgacac gttgccgttc
     9361 gccgggcccg acgatctgct cagggtggac ggcatcgggc cgaagacgct ccagaaggtg
     9421 cggccgttca tcaagatcgc cggcccggaa acacccgccc catgaacacc ccgtcgttcc
     9481 ttgtcggcct cgatctgcag cccgactcct gccgggcggt ccggatgcgt gtggacacgc
     9541 cgcgaccgga ggtggccgcg gtggctgaga tcactcccgg ccggccggcc ccggaagagt
     9601 tttttgcaga agccgcgctg gtcgccgcgg tccccggcca gtatgcgatc gccaagaaca
     9661 tccccctcac ggatggaggc tcggacccct gcgcgcgagc gctgttcgag ctggcgcagt
     9721 cactgaccgg gagccccacg gattattggc tcgatgccgc accgaccggt ttggcgggat
     9781 tgatgctggg gatggcggtg cggcggagcg tgctccggca ggagcttctt gatccgctgg
     9841 cgggcatgac cggccgcacg ccgatccggg ccgaggtccg gggcctggcg ctcgcgcacg
     9901 gctatctcgc cttctgccgc ccctcgcggg gaggcgtgct ggtcatgatt gaccaggacg
     9961 gagggcacgg ttcggcggcc gtcctgtatc gcaaccgggt ggttgcgctc gcccacgtgc
    10021 cgaccgggca tctcgacctg tcggcggcgg ccgaccgcga gc
//
LOCUS       DAOPOE010000100        10011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7658_length_10011_cov_4.420149, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000100 DAOPOE010000000
VERSION     DAOPOE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7658_length_10011_cov_4.420149"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..429)
                     /gene="rpmA"
                     /locus_tag="PKM94_11455"
     CDS             complement(160..429)
                     /gene="rpmA"
                     /locus_tag="PKM94_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOD67291.1"
                     /translation="MAHKKGVGSSKNGRDSRGQRRGTKLYDGQLISAGSIIVRQCGTR
                     IRPGANVGIGKDNTLFAKIDGIVKFQRVGREHKQVSVFAAAPETA"
     gene            750..1601
                     /locus_tag="PKM94_11460"
     CDS             750..1601
                     /locus_tag="PKM94_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67292.1"
                     /translation="MRRWYIGHEQKRRYQGSFLLACLATYAIVIGGSLFLLSGSGVER
                     PAPAAGAAAAGAPTASAERPAAAGTRLPRPMSGGGAPGGKFRLGFVGAVRIVRDVEWP
                     RISVPKRGREDPPAAPMLAQLDFPATTGGGRGDSYGLGDDPFGLAPSEPYLPATRRAP
                     QWGMEQLPRGETRDAAVEFWAPSWPPGARLGRERRDTAIVYVWLLIRPDGTVRYRILS
                     ETPAGQGFGREVESALLRSRYTPPVREGVKDSVAFVLRVLLCEGCDSYMTEEPPNIRA
                     SMRQPRR"
     gene            complement(1696..4164)
                     /locus_tag="PKM94_11465"
     CDS             complement(1696..4164)
                     /locus_tag="PKM94_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOD67293.1"
                     /translation="MNEMFTEGARKAIEYARDEASRLRHDYIGTEHLLLGLIRLGEGR
                     AVEIIVNLGLDLNDLKQSVEEVVQPSGGTMTMGQLPLTARAKKTLEVSGQEARALKSK
                     DIDTEHILLALLKDEEGVAAQVLSTYEIDYKEAYEELKNIQNGRPSSYKKKRKKSKTP
                     ALDHFGRDLTELARRGKLDPIVGRANEIERVAQVLSRRKKNNPVLIGEPGVGKTAIVE
                     GLAQRIVENRVPQTLENKRVVTLDMASLVAGTKYRGQFEERLKAVLNEIINAEDVIIF
                     IDEIHTIVGAGGAEGSLDASNIFKPSLARGELRCIGATTMNEYRKYIEKDGALDRRFQ
                     TVMVDPPSEEDTIRILKGLRSQYEKHHRLVISDEAIEAAVKMSNRYITGKFQPDKAID
                     LIDEAGSRAHLATFTRPARFAEIEGQIQELQQRKEKSVKNQAFETAAQLRDELKAKKD
                     ELARLTETWEKTRESETVTLTADDVATVLSKITGIPLFRLEESESKRLLRMEEELRKA
                     IVGQDEAISAISKAIRRARAGLGDPRRPIGTFMFLGPTGVGKTELARALAMFLFEDAN
                     SLIRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVVLLDEIEKAHPD
                     VFNILLQLFDDGALTDSFGRKVDFKNTVVIMTSNLGTRQLRDQKTVGFDAEAVDLGYD
                     SMSRKIREELKKAFNPELLNRIDETVVFRSLTKEDIKQIIDIQVQDTAKQLAEKGIAF
                     KLTDSAKEFICEKGWEPEYGARPLRRSLRKYLEDPLSEELLRGQYAGDCELVVTAGRT
                     ELIFSFNTKEPEAAGSGGQHALER"
     gene            complement(4240..5289)
                     /locus_tag="PKM94_11470"
     CDS             complement(4240..5289)
                     /locus_tag="PKM94_11470"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOD67294.1"
                     /translation="MFEDMVKSPAVWLSGKGEESAAVLSSRVRLARNIAGCKYPPSAD
                     SETGTRIVNYVDSTVSRSDLLGRGRYVRAGDISPLDRDFLVERHLISPAFLSGDLPKA
                     LYIGSQEQVSIMVNEEDHLRIQALASGLDPRGAYAAAKQYDQEIGRHLEYDYDPDFGF
                     LTACPTNAGTGMRASVLIHLPGLVLTREIDHVIRHITATGLIVRGFYGEGSDVLGNLF
                     QISNQNTLGITEDDILAQIMRVTRDIIEKEAAARRRLVDEASDMIEDKIWRAYGILKH
                     ARVLTSEEVMNLLSAVRLGQAMGIIDFLSVAQINDMLLLSQPAHLQRFYGSEMDNNRR
                     DFVRAQMVREKLRNT"
     gene            complement(5318..5848)
                     /locus_tag="PKM94_11475"
     CDS             complement(5318..5848)
                     /locus_tag="PKM94_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOD67295.1"
                     /translation="MLCQDCNKNEATVHLTQIVNNQKTMLNLCKQCAAARGFHSPLEN
                     VPFPLAEILSGFASSPQGAPPVDTGDPLTCPGCGLTFQAFTRQGRFGCGECYKAFRSR
                     LEPIMRKIHGASLHRGQRPVTVIDGAADDDAIPVKEEERLEAELAKAIESEDFERAAE
                     IRDKLKTIREESSLHR"
     gene            complement(5867..6559)
                     /locus_tag="PKM94_11480"
     CDS             complement(5867..6559)
                     /locus_tag="PKM94_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD67296.1"
                     /translation="MDTHDDTILRAVDLHREFRTPDATLHVLRGINLTMPRGDTVAVT
                     GASGVGKSTLLHLLGGLDRPTAGAVEIDGTRVDNQSEADLAAFRNRNVGFVFQFHYLL
                     EDFSAAENVMMPVLVAGQSRTQARRRAELLLDMVGLSERKNHRPAQLSGGEQQRVAVA
                     RALANDPRIVLADEPSGDLDSATGRMLHELLFRLNREQSIALLIATHNRELADRCNRT
                     LEIVEGKFCERS"
     gene            complement(6659..7885)
                     /locus_tag="PKM94_11485"
     CDS             complement(6659..7885)
                     /locus_tag="PKM94_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD67297.1"
                     /translation="MGYERFVARRYLRSGRFFISVSTWITILGVALGVGVVCFVMSMH
                     NGFEAEIRTRLLGTTSHISIFPVREEFIANYRALVDSLARVPGVAAASPFIYYKAAIA
                     SESAGDGIIVRGIVPELERRASQIEKNIVGGTYNFNEIVIADDTVPGIILGKNLADRL
                     GAVLGSEVVLYSLKGDEIRSNTRPRVAKFYVAGIFESGMFEFDSELAYISLPAAQRLF
                     RTGDAVTTIHLRLDDVYRAEAIRPVVDSLLEHRFEVVPWNVLHRNLFAWIAIEKKVLF
                     LGFILIVLVAAFSIISTLVMLTMEKRAEIGILKTIGSTPGSIRKIFVYKGLGIGAGGV
                     AGGWGLALAAAWLQNRYHLVSLPPDIYFISYLPIDIHPVDFLLAGLVTVAICFLAALY
                     PAHQASRVSVVEVLRR"
     gene            8138..8491
                     /locus_tag="PKM94_11490"
     CDS             8138..8491
                     /locus_tag="PKM94_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOD67298.1"
                     /translation="MGQPVVHFEIAGRDYRKSIAFYRNLFDWTIEEVGAFPYGMVQAA
                     GERAIGGGISPVAPGGRPGVTVYVMVDDLQTYLERAQQLGGKTVTPPSPIPGIGEFAM
                     FADPDGNVIGLFKAK"
     gene            8605..8985
                     /locus_tag="PKM94_11495"
     CDS             8605..8985
                     /locus_tag="PKM94_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD67299.1"
                     /translation="MTQAPAAARVAVLGASPKPDRTSFQAVRLLKEHGFHPIPVHPAG
                     HAVDSVPGVSSLDEVAPPPDTLTVYVNAGISSDLRDAMLRLRPRRVIFNPGAENDDLA
                     AELRAQGIEVVEACTLVMLRTGVF"
     gene            9097..>10011
                     /locus_tag="PKM94_11500"
     CDS             9097..>10011
                     /locus_tag="PKM94_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67300.1"
                     /translation="MSTIREIRGSAMQIVRLAAAVAALAMVGLPVAGLSADLKIEKAY
                     LAYQGCPGDVSVSVENLDFALGRFRLLIAVNEAATAPVRVDPGATLAACGYEQFGYRL
                     ITDTAGNYDLGPFTRLIEITGTLAAPGGDYPCAGAGATVELARLHLLATNDRDWECLA
                     VPVVFFWRDCLDNALVSRQGDTMEAVGALFSLQGEDISTFYAFPGFGPPDPSCEGPAG
                     VTLVRGLNGHWGGVDFVCSDRAWDCNDFSGDLNLNGFRLEASDLVLYADYFQFGLGVF
                     RVSVEGQIAASDIDCDGSTLSVHDLYAMM"
BASE COUNT         1664 a         2958 c         3520 g         1869 t
ORIGIN      
        1 cggcgacggc ggacgaagtg ctggcgctgg cggaaaagat cgattttgca ggggcggtcg
       61 aggcaatgcg ctaagctggc gggaccacca aaaaaagaaa acgccgtccg gtcgaccggg
      121 cggcgtttcg catttatagc gtctggcgcg gtgcgccgat caggcggttt ccggggcggc
      181 ggcgaaaacc gacacctgct tgtgttcgcg cccgacccgc tggaatttga cgatgccgtc
      241 gatcttcgca aagagcgtgt tgtccttgcc gatgccgacg ttcgcgccgg ggcggatgcg
      301 ggtaccgcac tgacggacga tgatcgagcc ggccgaaatc aactggccgt cgtacagctt
      361 ggtcccccgc cgctggccgc gggagtcacg gccgttcttg gaggagccga cgcctttctt
      421 gtgggccatg gtaaacctct atcagtctct cgttttcttc tcgcctctcc ggaccaggga
      481 acatcggggg cgcggtcagg ttcccgaccg cggtgacccc cggcgacagc caaccaatat
      541 ataaaacccg cgcccaaagg caagtggaaa gtgtaacgca ctctctgcgt gacgcttaca
      601 tcggcgtccg ggcggtgagc cgcccccgtg cggagacgcg ccgtggtcgt ctgcctgcaa
      661 atcctctgca cgatccccaa gaggagcgcg tcttccaggc aggcaggcaa cggacgaaaa
      721 tcaccgggaa ctacgccaca ggtgaggcga tgagacgctg gtatatcggg cacgagcaga
      781 aacggcggta ccaggggagt ttcctgctgg cgtgtctggc cacttacgcg atagtcattg
      841 gggggagtct gtttcttctg tcgggatccg gggtggagcg cccggcgccg gcagcgggag
      901 cagcggccgc gggggcgccg acagcctcgg ccgaacgccc cgccgccgcc ggcactcgtc
      961 tgccccggcc gatgagcggg ggaggcgcgc cgggcgggaa attccggctg ggctttgtgg
     1021 gcgcggtgag gattgtgcgc gatgtcgagt ggccgagaat ctcggtcccg aaacggggcc
     1081 gcgaggatcc accggccgca ccgatgctgg cgcaacttga cttccccgcc acgaccggcg
     1141 gcggccgggg ggacagctac ggcttagggg atgatccgtt cggcctggcg ccgtcggagc
     1201 cgtacctgcc ggccactcgg cgtgcgccgc aatgggggat ggagcagctt ccgcgaggcg
     1261 agacgcggga cgccgcagtg gaattctggg cgccgagctg gccgccgggg gcccgcctcg
     1321 gccgcgagcg gcgcgatacg gcgatcgtgt atgtctggct gctcattcgt ccggacggga
     1381 cagtccggta ccggattctc agcgagacgc cggccgggca gggattcggc cgggaggtgg
     1441 agtcggcgct cctgcggagc cgctacacac ccccggtccg cgagggagtc aaggactcgg
     1501 tggctttcgt gctgcgcgtg ctcctgtgcg aaggatgcga ttcgtacatg accgaggagc
     1561 cgccgaatat ccgcgcgagc atgcggcagc cgcggcggtg atgaccggcg gtgcggccgg
     1621 agaaaacgtg aaacggccgg gagcaagagg ctcccggccg tggtgttgac gggggctatt
     1681 ccggcgccgg cgcggctagc gctcgagagc gtgctggccg cccgaaccgg ccgcctcagg
     1741 ctctttcgtg ttgaagctga agatcagctc cgtgcggccg gcggtgacca ccagttcgca
     1801 gtcgccggcg tactgcccgc gcagcaactc ctcggagagc gggtcctcga ggtacttgcg
     1861 cagcgagcgg cggagcggcc gggcgccgta ctcgggttcc cagcccttct cgcagatgaa
     1921 ctccttggcc gagtcggtca gtttgaaggc gatgcctttc tccgcgagct gcttggcggt
     1981 gtcctgcacc tggatatcga tgatctgctt gatatcctcc ttggtcagcg agcggaacac
     2041 gaccgtctcg tcgatccggt tgagcagttc cggattgaag gccttcttga gctcctcgcg
     2101 aatcttccgg gacattgagt cgtaccccag atccaccgcc tcggcgtcga acccgaccgt
     2161 cttctggtcg cgcaactggc gggtgccgag gttggaggtc atgatgacca cggtattctt
     2221 gaagtcgacc ttgcggccga agctgtcggt gagcgcgccg tcgtcgaaca actggaggag
     2281 gatgttgaag acgtcgggat gggccttctc gatttcgtcg aggaggacga ccgagtaggg
     2341 tttgcggcgg accttttcgg tcagctggcc gccctcttcg tagccgacgt atcccggggg
     2401 cgctccgatg aggcgggaga cggcgaactt ctccatatac tcggacatgt cgatgcggat
     2461 gagggagttg gcgtcctcga agaggaacat ggccagggcg cgggcgagtt cggtcttgcc
     2521 gaccccggtc gggccgagga acatgaaggt gccgatcggc cggcgcgggt cgccgaggcc
     2581 ggcgcgggcc cggcggatgg ctttggagat ggccgagatg gcctcgtcct ggccgacgat
     2641 cgccttgcgc aactcttcct ccatgcgcag gaggcgcttg gactcgctct cctcgaggcg
     2701 gaagagaggg atgccggtga ttttcgacag cacggtcgcg acatcatcgg ccgtcagggt
     2761 gaccgtctcg ctctcgcggg tcttttccca ggtttcggtc aaacgggcga gttcatcctt
     2821 tttcgctttc agttcatcgc gcagctgggc ggcggtttcg aaagcctggt tcttgaccga
     2881 tttctccttg cgctgctgga gttcctggat ctggccttcg atttccgcga acctggccgg
     2941 gcgggtgaag gtggcgaggt gggcgcggga gccggcctcg tcgatgaggt cgatcgcctt
     3001 gtccggctgg aatttgcccg taatataccg gttggacatc ttcaccgccg cttcaatcgc
     3061 ctcgtcggag atcaccaggc ggtggtgttt ctcgtactgc gagcgaagcc ccttgaggat
     3121 gcggatcgtg tcttcctcgg agggggggtc gaccatgacc gtctggaagc ggcggtcgag
     3181 agcgccgtcc ttctcgatgt acttccggta ctcgttcatg gtggtggcgc cgatgcagcg
     3241 gagttcgccg cgggcgagcg agggcttgaa gatgttggag gcatcgagtg aaccctcggc
     3301 gccgccggcg ccgacgatgg tgtggatttc atcgatgaag atgatgacgt cctcggcgtt
     3361 gataatctcg ttgaggacgg ctttgaggcg ttcctcgaac tggccgcggt acttggtgcc
     3421 ggcgacgagg gaggccatgt cgagggtgac gacccgtttg ttttcgaggg tctgggggac
     3481 gcggttttcg acgatgcgct gggcgaggcc ctcgacgatg gcggtcttgc caacgcccgg
     3541 ttcgccgatg aggacggggt tgttcttctt gcggcgcgag aggacctggg cgacgcgctc
     3601 gatttcgttg gcgcggccga cgatgggatc gagcttgccg cggcgggcca gctcggtcag
     3661 gtcgcggccg aagtggtcca gcgccggagt cttcgacttt ttgcgcttct tcttgtaaga
     3721 ggaaggacgc ccgttctgaa tgttcttgag ctcctcgtag gcctctttgt agtcgatctc
     3781 gtaggtggag aggacctggg cggcgacgcc ctcttcgtcc ttgagcagcg ccaggaggat
     3841 gtgttcggtg tcgatatcct tcgatttgag cgcccgcgct tcctggccgg acacctcgag
     3901 ggtcttcttg gcccgggcag tgaggggcag ctggcccatg gtcatggtgc cgccggaggg
     3961 ctggaccacc tcttccacgg actgcttcag gtcgttgagg tcgagtccga ggttgacgat
     4021 aatctcgacc gctcggccct cacccagccg gatcagtccc agcagcaggt gttcggttcc
     4081 gatgtagtcg tgccggagcc gggaggcctc gtcgcgggcg tactcgatgg ctttgcgggc
     4141 accttcggtg aacatctcat tcatcgcgaa ccttccttgc gtctatgtcc aagacttctt
     4201 gtcacgccaa tatgcacctg taaatacatc atatcgggcc tatgtgttcc ggagtttctc
     4261 gcgcaccatc tgggcgcgga cgaaatcgcg ccggttgttg tccatctcgg agccgtagaa
     4321 ccgctggagg tgggcgggct gcgacagcag gagcatgtcg ttgatctggg ccacgctgag
     4381 gaagtcgatg atgcccatgg cctggcccag ccgcacggca gagagcaggt tcatcacctc
     4441 ttcggacgtg agcacgcggg cgtgtttgag gatgccgtaa gcccgccaga tcttgtcctc
     4501 gatcatatcg gaggcctcgt cgaccagccg ccggcgggcg gcggcctcct tctcgatgat
     4561 gtcgcgcgtc acgcgcatga tctgcgccag gatgtcgtcc tccgtgatgc cgagcgtgtt
     4621 ctggttggag atctggaaga ggttccccag cacgtcggaa ccctccccgt aaaacccgcg
     4681 cacgatgagg ccggtcgcgg tgatgtggcg gatgacatgg tcgatctcgc gggtgaggac
     4741 gagcccgggg aggtggatga ggaccgaggc gcgcatgccc gtgccggcgt tggtcgggca
     4801 ggccgtgaga aaaccaaagt ccgggtcgta gtcgtactcg aggtggcggc cgatctcctg
     4861 gtcgtactgc ttggccgccg cgtacgcacc ccgcgggtcg agtccggagg ccagcgcctg
     4921 tatccgcagg tggtcttcct cgttgaccat gatcgacacc tgttcctgcg acccgatgta
     4981 gagggctttg gggaggtcgc cggagaggaa ggcgggggag atgaggtggc gctcgacgag
     5041 gaagtcgcgg tcgagggggc tgatgtcgcc ggcgcggacg tagcggccgc ggccgagaag
     5101 gtcggagcgg gagacggtgg agtcgacata gttcacgatg cgggtgccgg tttcgctgtc
     5161 ggccgagggg ggatatttgc agccggcgat gttgcgggcc aggcgaaccc ggctggacag
     5221 cacggcggcc gattcctcgc ccttgcccga cagccagacg gccggtgact tgaccatatc
     5281 ttcaaacatc gtccatcttc cccatctccc cccgcggcta ccggtgaagg gatgattcct
     5341 cgcggatcgt tttgagtttg tcgcggattt cagcggcccg ctcgaaatct tcgctctcga
     5401 tagctttggc cagctccgcc tcgaggcgct cctcctcttt gacggggatg gcatcatcgt
     5461 ccgccgcgcc gtcgatgacg gtgacggggc gctggccgcg gtggagggag gcgccgtgga
     5521 ttttgcgcat gatcggttcg aggcgggagc ggaaagcttt gtagcattcg ccgcagccga
     5581 agcggccctg gcgggtgaaa gcctggaagg tgaggccgca gccggggcag gtgagcgggt
     5641 cgccggtgtc gaccggcggc gcgccctggg gggaggaggc gaagccggag aggatttcgg
     5701 ccagggggaa gggaacgttt tccagggggg agtggaagcc gcgggcggcc gcgcactgct
     5761 tgcacaggtt cagcatcgtc ttctggttgt tgacaatctg cgtgaggtga accgtcgcct
     5821 cgttcttgtt gcagtcctga cacaacatag tcacattccc atgcggttac gagcgctcgc
     5881 agaacttgcc ttcgacgatc tccaaagtgc ggttgcagcg gtcggccagt tcgcgattgt
     5941 gggtcgcgat gaggagggcg atgctctgtt cgcgattgag gcggaagagc agttcgtgaa
     6001 gcatccgtcc cgttgcactg tcgaggtcgc cggagggttc gtcggccaga acgatccggg
     6061 gatcgttggc cagggcccgg gcgaccgcca cccgttgctg ttcaccgccg gagagctgcg
     6121 ccgggcggtg gttctttctc tcacttaaac cgaccatgtc gagcaacagt tccgcccgcc
     6181 ggcgggcctg ggtgcggctc tggccggcga cgaggacggg catcatgacg ttttcggccg
     6241 ccgagaaatc ctccagcaag tagtgaaact ggaacacgaa accgacattg cggttgcgga
     6301 acgccgccaa gtccgcctcc gactggttgt ccacccgggt cccgtcgatc tcgactgccc
     6361 cggccgtcgg ccgatccagg ccgccgagga ggtgcaggag ggtcgatttg ccgaccccgg
     6421 aggcgccggt gacagccacg gtgtcgccgc gaggcatggt caggttgatg ccgcgcagga
     6481 cgtgcagggt cgcgtccggg gtccggaact cgcggtggag gtcaacggct cgcaagatgg
     6541 tgtcgtcgtg ggtgtccatc gttcggttta ctcgtcctct tgggccccgt tacgggcata
     6601 cagtagtatt ttcgccagaa ttccgtaaac gtttaccatt gcgcgtgttt tccgccattt
     6661 agcgccgcaa aacctccacg accgacaccc gcgaggcctg gtgggccgga tacagggcgg
     6721 ccaaaaagca gatcgccacc gtcaccaggc cggccagcag gaaatcgacc gggtggatat
     6781 cgatcgggag gtagctgatg aaatagatgt cggggggcag gctgaccaga tggtaccggt
     6841 tttgcagcca ggcagccgcc agcgccagcc cccagccgcc cgcgacgccc cccgcgccga
     6901 tccctaagcc cttgtacacg aatatcttgc ggatcgatcc gggagtcgaa ccgatcgttt
     6961 tcagaatgcc gatttcagcc cgcttctcca tcgtcaacat aaccagtgtc gagatgatgg
     7021 aaaaagcggc aaccagcacg atcagaatga accccaggaa gagcaccttc ttctcgatgg
     7081 cgatccaggc gaacagattc cggtggagga cgttccatgg gaccacttcg aaacggtgct
     7141 cgaggagtga atcgacgacc gggcggatcg cctcggcccg gtagacgtcg tcgaggcgca
     7201 ggtggatggt ggtgacggcg tcgccggtac ggaagagtct ctgggccgcg ggcagcgaga
     7261 tgtaggcgag ctcggagtca aactcgaaca tgccgctttc gaagatgccg gcgacataga
     7321 acttggcgac ccgcgggcgg gtgttgctgc ggatttcatc gcccttgagg gagtagagga
     7381 caacctcgga gccgaggacg gcgcccagac ggtcggcgag attcttgccg aggatgatgc
     7441 ccggcacggt gtcgtcggcg atgacgatct cgttgaaatt gtaggttccc ccgacgatgt
     7501 tcttttcgat ctgggaggcg cggcgctcga gttcggggac gatgccgcgc acgatgatgc
     7561 cgtcaccggc gctttcggaa gcgatggcgg ccttgtagta gatgaagggc gaggcggcgg
     7621 cgacgccggg aacgcgggcg agggaatcga ccagggcgcg gtaattcgcg atgaactcct
     7681 cccgaaccgg gaagatcgag atgtgggagg tggtgccgag cagacgggtg cggatctcgg
     7741 cctcgaaccc gttgtgcatg gacatgacga aacagacgac gcctacgccg agggccaccc
     7801 cgaggatcgt gatccaggtc gagacggaga tgaagaaccg ccccgaccga agatatcgtc
     7861 gcgccacaaa acgttcatag cccatgcatc catccatccc tggtgcaaaa ccggcacggc
     7921 actcattgtg aaggccggtg cgcagcggca ctcagggtat tatacgcccc gccggaggca
     7981 acgcaatctt tttcggaccg gcggaccatg cggccccctt gacagcagac ggagcacatg
     8041 cgaagttgag gccggcaaga acctgaagta tccggtcagc agtcaaccga gccgcgggtg
     8101 ggggcgtccg acccggccgc ggctgaagga ggagcaaatg ggacagccgg tcgtgcattt
     8161 tgaaatcgcc gggcgggact accggaaatc aatcgcgttc tatcgaaacc tttttgactg
     8221 gacgatcgaa gaggtcggcg cctttcccta cggcatggtg caggcggccg gagagcgggc
     8281 catcggcggc gggatcagcc cggtcgcgcc gggcggtcgg cccggggtga cggtgtacgt
     8341 gatggtcgac gatctccaga cctacctcga gcgggcgcag cagctcggcg ggaaaaccgt
     8401 gacgccgccc tcgcccattc ccggcatcgg ggaattcgcc atgttcgccg atcccgacgg
     8461 gaacgtgatc gggctgttca aggcgaagta ggcgccggac cgcacccgcc gccggaaccc
     8521 cggcggcagt cggcgtgttg gggggcaggg ggatgacaga ttgactttta aacgtgcttg
     8581 cgacaaccag gaggaacgat cgccatgacc caagctcccg ccgccgcccg cgtggccgtg
     8641 ctcggcgcat cgcccaaacc ggaccgcacc tctttccagg cggtccgtct gctgaaggaa
     8701 cacggctttc accccatccc ggtccacccg gccgggcatg cggtcgacag cgtcccgggg
     8761 gtgagctcgc tggacgaggt agcgccgccc cccgacacgc tcaccgtcta tgtcaacgcc
     8821 ggcatttcct cggatctccg tgacgcgatg cttcgccttc gcccgcggcg cgtgatattc
     8881 aaccccgggg cggagaacga tgatctggcc gcggaactgc gggcgcaggg gatcgaggtg
     8941 gtggaggcgt gcacgctggt gatgttgcgg acaggtgtgt tctgaccgcg gagtgtgccc
     9001 gggcaaacgg gggcgcgcgc aggggccgcg agggacgcgc ataaaaccgg ttgacgatcg
     9061 gggctgtttg cggtatcttg tggcaaggat accgtcatgt ccacgatccg tgaaatccgg
     9121 gggagtgcca tgcagattgt cagattagcc gccgcggttg cggcgctcgc aatggtcggc
     9181 ctgccggtgg ccggtctgag cgccgatctg aaaattgaga aagcgtatct cgcctatcag
     9241 ggttgcccgg gggacgtctc cgtttccgtt gagaatctcg atttcgcgct gggtcggttc
     9301 cgcctgctga ttgcggtcaa tgaggcggcg acggcgccgg tgcgggtgga tccgggagcc
     9361 acgctcgcag cgtgcgggta cgagcagttc ggctaccgcc tgataaccga taccgcgggg
     9421 aactacgatc tgggaccgtt cacgcggctg atcgagatca cggggacgct ggccgcgccg
     9481 ggcggcgact acccctgcgc gggtgccggc gcgacggtcg aactggcccg cctccacctc
     9541 ttggccacca acgatcggga ttgggagtgc ctcgccgtgc cggtggtgtt tttctggcgg
     9601 gattgtctcg acaacgctct tgtcagccga cagggagaca ccatggaggc ggtcggggcg
     9661 ctgttctccc ttcaagggga ggacatcagc acgttttacg cattccccgg tttcggtcct
     9721 ccggatccat cgtgtgaggg gcccgcgggg gtgacgctgg tgcgcggact gaacggccac
     9781 tgggggggag tcgattttgt ctgctccgac cgcgcgtggg actgcaacga cttcagcggc
     9841 gacctcaatc tcaacggctt ccggctggag gcctccgacc ttgtcctcta tgcagactac
     9901 ttccagttcg ggctgggtgt gttccgggtg agtgtcgagg ggcagattgc ggcgagcgat
     9961 atcgactgcg acgggagcac cctctcggtt cacgacctct acgcgatgat g
//
LOCUS       DAOPOE010000101         9873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7799_length_9873_cov_5.545223, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000101 DAOPOE010000000
VERSION     DAOPOE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_9873_cov_5.545223"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..934
                     /locus_tag="PKM94_11505"
     CDS             146..934
                     /locus_tag="PKM94_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOD67301.1"
                     /translation="MQPVLKIVLVGSLLLNLVAIWGFYHYVRYGGSPLGELKRILTRS
                     THQPAPAIPFLEENERIKTQLAAGSAPPNRVVFFGASITARWKLDSAFPGVAAINRGV
                     GNQLVPAMLARFKRDVLELQPDAVAIKFCSINVRPFMPLSVMQDGMAMMVELARAHDI
                     QPIVCTIIPAGRPEAAIGDFSVADTLQKFNAWARTWAAAENLPLIDYARALSDAEGHL
                     PREYSVDPVHVNDKGYEIMAAAAQPVIDSVLRTRPGSGPARTVR"
     gene            971..2038
                     /locus_tag="PKM94_11510"
     CDS             971..2038
                     /locus_tag="PKM94_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD67302.1"
                     /translation="MKRLARTLAAFAAHRLRFADLPYALHRLVRGQYVVAAFVYHRIV
                     DSRATASFYTDYERGLDTGIFSRHLDVLTRHFHMVDLDTFLAIVSGRRPAETHSALLT
                     FDDADAGFIDYALPELRRRDIPAAIMAPVQLIGTADQFWHVRVSNAIRHADDRHWDEI
                     VRRAGEWPAPVAAVVRRYSALPPPARRRPLARAVNLALDQVHHDDIDRLLAAWQRLIA
                     PAAPVPVYCMDWEALRYLERHRIAIESHTLSHRKLPYLSDEEIACELSQSKLTLEEKL
                     GKRVRAVAYPQGRYERRIGDAARAAGYEAGFTTVRLPCRYPLAPENMFALPRLDLGGE
                     TYRELEFHLAKAGTRWIRQTP"
     gene            2035..3588
                     /locus_tag="PKM94_11515"
     CDS             2035..3588
                     /locus_tag="PKM94_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOD67303.1"
                     /translation="MTIDPKQRLLRNVTSNWVQMAIYTVIALVMTPFVVHALGKDVYG
                     IWALIFSIIHYTNFFDVGFKQSLARYLPKYYATQDYEKLNEVINSSSVIYAVSGTLVL
                     ITAAIIAAFFVDYFAVSEDLLPLMRIALIIMGVNQAMYFYLMASTALGPFHRYDLLNA
                     IEIGRTLVGAACTIVLLMQGYGLIALALVTLLTNFGSLLLKRFFQHRIVPQIEYNRKY
                     VNRQRIRELFNYGLVSFLIVMAWIVIFSTDNIVVGMFISTQAVAYFNIAGTIINNLRS
                     IVASIGTPLVPVISHLDATSDLAEIAALHRKTVRYLYYISTLIGVGVFFFGRQFVGLW
                     MGPGFDTTVTILFILIAPACIYLPQMITNSVLLGIGRHPTLLYILGVEALANIGLSLA
                     LVHVWGVYGVALGTAIPQVVIYSFVYPYVFHRTIRARLSDFYRDSGLSIATALLFTLP
                     VAWAVSYLPLPGWVGFLAQISAVTAVSLAGFAWRILDDADRRRFLGRFFRSRAAGPQP
                     PEKKTAPGR"
     gene            3607..4956
                     /locus_tag="PKM94_11520"
     CDS             3607..4956
                     /locus_tag="PKM94_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOD67304.1"
                     /translation="MGLYNLFHSTLFRLKDLRDRTGVYRLAARLRREQFLPRPDLDDL
                     RRRRLLDLLAHARLHSPYYRDLLAGLEPDLERIAPHEILSRIPLLTREIVQEEGSRLL
                     CPGIPGSYPDSSGGSTGRPVNFHHDLTYLNWGRAHELFFLSWMGVRPGDRTAVFWGAD
                     RDLKEESLYTRLMLRLNRVRQLNSFAMSEAQIDAFLKQLNAFRPRYIYGYASSLYHVA
                     TFIARGRPLGFRPLAIRSSAEMLYPVQRAAIEQAFDAPVHNFYGSREVNNIAAECAAH
                     DGLHIFASARMVEIVDDRGRLLPPGEVGHIAVTDLTNRAFPFIRYLNGDLAALKSGLC
                     PCGRTYPRLEKIAGRSSDIITVEGRHIHGEYFTHLFYGRPEVKAFQLIQETEYDFRLL
                     LVPRTRDLDTAFFRDRIRDKLGPRVRLEIALVDDIPPTAGGKHRFTVSKVPPRKDGL"
     gene            5024..6502
                     /locus_tag="PKM94_11525"
     CDS             5024..6502
                     /locus_tag="PKM94_11525"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD67305.1"
                     /translation="MATTTHHLPGGSRAYLRPEHLAYAAFAAAMILSGLLVALPFPYH
                     ILPFALLGVVVFVIGTLRYPYFGLYLYLIIFFMDPNQIFPFMQTVAFPYEKVLAFAVI
                     GILLIHIAILKKEFDLYRLDVGFAAMILATYLSILGAVDLAAAWEDWQRFLRLFLVYL
                     VMVRIVKTEGQLKAIIMLFIISIGFLAISSTIQYYRGEFEVRQGIQRLHSFGGGLIDP
                     NTMASSLVLGVPFMFYMAKAHRSALLKLFMFALIGSCFWAIMLTGSRGGMVGGIALML
                     LLVWHSRHKTLGFVVAFAVLVCIAAVMPDQYQNRFMSIFRINEQDEFGASDSARGRIN
                     GFKLGFKFLLKNPLTGVGISNFGWHHHLEPNGDWTDAHNLVGKLVGELGLIGVAAFSF
                     FIARFAGTLKLIKRLYDEHGWPPDFLFQMRQAIKIGLIMLFVQGLFGHNLFRDNWYFT
                     AGFIVIVSKIVWDRHLAAQRPSGATPGSPEPLPGAEKGLLPG"
     gene            6499..7884
                     /locus_tag="PKM94_11530"
     CDS             6499..7884
                     /locus_tag="PKM94_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67306.1"
                     /translation="MRLTRFPARPRGPLELFAALNRYALAAYRGERAIYPLVRELERN
                     FSLSRDELEAAQDKALAAIVDHAFAHSPFYRRKFAAVGFKPGDVKGRADLALLPETNK
                     IELVRAADEVLCLPKDRLRAAGTGGTSGIPFTFYRDPRCLLFRRAVDYTTFRYYGWRA
                     GQWQGWLWGAPRDLVAPATARGRLLQRWAVRTFFADVTRLGTESYRSFAALARRYRPT
                     WLSAYPSLAYDLAAAIEAGDIEPFRVPMVNLTAEPVYDFQKQLIRATFGEEVCERYGS
                     REMGLAALECPAHNGLHYFMESVYFESIDRGPGSPGRSLVVTDLVNRGMPFIRYDTGD
                     FARLDDAPCTCGRTTPRLIDIRGRETDNIWCPDGHGIAGAIVVYLFARINPGVPMQLM
                     QESLDRIVIRIAGRPGELAGRAAELIDVCRREISPDIAYTIEYVDHIERAPSGKYRYI
                     ISRVPPPSRRR"
     gene            8045..9265
                     /locus_tag="PKM94_11535"
     CDS             8045..9265
                     /locus_tag="PKM94_11535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67307.1"
                     /translation="MDETTSRLRLLLIDEEFPYPLTSGKRLRTYNLLRELSRRCDITT
                     LAYGDPDSEGFRRFERDGLTPVAVPAPDRRQSGLRFYWRLFGNLFSPYPYIVTSHTTR
                     RFHRRLRQLLARHSFDLVLCEWTPYARFVKDLPGVRKVITAHNIEAAIWRRYEQQETQ
                     PLRRLYIRLQRTKVEAFEARCFGWVDGAIAVSESEARQIAELGVPYPPAVVENGVDVG
                     YFTAPADDPDPGQIVFSGAMDWRPNQDAALWFVQEIFPLLRRARPNLAVTIVGRDPVR
                     RIRELGRVEGVTVTGTVADVRPYLARAAVFIVPLRIGGGSRLKILEAMAMRKAVVSTS
                     VGAEGLRVAADENIMIADDAPAFAQAVLRCLGEPDLRRRLGENGRRLVEREYRWDILG
                     HKMYEYLCRVMQQK"
     gene            9241..>9873
                     /locus_tag="PKM94_11540"
     CDS             9241..>9873
                     /locus_tag="PKM94_11540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD67308.1"
                     /translation="MPRDAAKVSVLHLRASNFYGGPERQLHCHARAARDSVFEVIVAS
                     YAESGRSPDLLARVAADRLPTHLVGVAGAYDRRALGLIRRVLRDRDIAILCTHDYRST
                     VLGCLAVRFTPVRWIAFSRGRTRETLRIRLYNELERLCLRFADRVVAVSEGEKASLRR
                     RLVPDRRIAVVHNAIDTAAFAAVTPVDLKSRFGFPAHSLVAVAAGRFSPEK"
BASE COUNT         1544 a         3895 c         2600 g         1834 t
ORIGIN      
        1 cgccctcgtc ctcgccaacg tcggcaaaaa ctgcaccgtc ggcgccggca gcgtcatcta
       61 caaggacgtt cccgacgacg ccaccgtcct gggcaacccg gcccggaagg tcaacctgga
      121 ataatctgac ggaggctacc atactatgca gcccgtgctc aaaatcgttc tcgtcggctc
      181 gctcctgctc aacctcgtgg ccatctgggg gttctaccac tacgtccgct acggcggctc
      241 ccccctcggc gagctcaagc gcatcctcac ccgctcgacc caccagcccg cccccgcaat
      301 cccctttctc gaagagaacg agcggatcaa aacccaactc gccgccggct ccgctccccc
      361 gaaccgcgtc gtcttcttcg gcgcctccat caccgcccgc tggaagctcg attccgcttt
      421 ccccggcgtg gccgccatca accgcggcgt cggcaaccag cttgtcccgg ccatgctcgc
      481 ccgcttcaag cgcgacgttc tcgagctgca gcccgatgcg gtcgcgatca agttctgctc
      541 catcaacgtc cgcccgttca tgcccctctc ggtcatgcag gacggtatgg cgatgatggt
      601 tgaactggcc cgcgcccacg acatccagcc catcgtctgc acgatcatcc cggccggcag
      661 gccggaggcc gccatcggcg acttcagcgt cgccgacacg ctccagaagt tcaacgcctg
      721 ggcgcgcacc tgggccgccg ccgagaatct cccgctcatc gactacgccc gcgccctttc
      781 cgacgccgag ggccacctgc cgcgcgagta ctccgtcgac cccgtccacg tcaacgataa
      841 gggatacgag attatggctg cggctgccca acccgtcatc gacagcgtgc tccggactcg
      901 cccggggtcc ggaccggcgc ggacggtccg gtagtccgct gaagagagta taaacagggg
      961 agattgtctc atgaaacgcc ttgcccgcac tctggccgcc ttcgccgccc accgactgcg
     1021 gttcgccgac ctgccctatg ccctccaccg cctcgtgcgc gggcagtatg tcgtggcggc
     1081 cttcgtctac caccgcatcg ttgactcacg ggccaccgcc tcattctata ccgactacga
     1141 acgcggcctg gacaccggga tattttcgcg gcacctcgac gttctcacgc gccacttcca
     1201 catggtcgac ctcgacacct ttctcgccat cgtcagcggc cgccgccccg cggaaaccca
     1261 ttcggcgctt ctcactttcg atgacgccga tgccggcttc atcgactacg cccttcccga
     1321 actgcggcgc cgcgatattc ccgccgcgat tatggcgccg gtgcagctga tcggcacggc
     1381 cgaccagttc tggcacgtcc gcgtctccaa tgccatccgc cacgccgacg accgccactg
     1441 ggacgaaatc gtccgccggg cgggcgaatg gcccgccccc gtagccgccg tcgtccgccg
     1501 gtactccgcc ctccccccgc ccgcgcggcg gcggcccctc gcccgcgccg tcaacctcgc
     1561 cctcgaccag gtccaccacg acgacatcga ccgcctcctg gccgcgtggc agcgcctcat
     1621 cgctcccgcc gccccggtcc ccgtgtactg catggactgg gaggctctcc gctacctcga
     1681 acgccatcgc atcgccatcg agtcccatac cctcagccac cgcaagctgc cctacctttc
     1741 cgacgaagaa attgcgtgcg aactcagtca gtccaagcta actttggagg aaaaactcgg
     1801 caagcgggtg cgcgcggtgg cctacccgca gggacggtac gagcggcgca tcggcgacgc
     1861 ggcccgggcc gccggctacg aggccggctt cacgaccgtc cgcctgccct gccggtatcc
     1921 gctggccccc gagaacatgt tcgccctccc gcgcctggac ctgggcggtg aaacgtaccg
     1981 cgagctggag tttcaccttg ccaaggccgg gacgcggtgg atcaggcaga ccccatgacg
     2041 atcgacccca agcagcggct cctccgcaac gtcacgtcca actgggtgca gatggcgatc
     2101 tacaccgtca tcgccctggt catgaccccc tttgtcgtcc acgcgctcgg gaaagacgtc
     2161 tacggcatct gggccctcat cttctcgatc atccactaca ccaatttctt cgacgtcggc
     2221 ttcaaacaga gcctcgcccg ctacctcccc aagtactacg ccacccagga ttacgagaaa
     2281 ctcaacgagg tgatcaactc cagcagtgtc atttacgcgg tctcgggcac gctcgtgctc
     2341 atcaccgccg ccattatcgc cgccttcttc gtcgactatt tcgcggtgag cgaggatctc
     2401 ctcccgctca tgcgcatcgc cctcatcatc atgggcgtca accaggcgat gtacttttac
     2461 ctcatggcga gcaccgcgct cggccccttt caccgctatg acctgctcaa cgccatcgaa
     2521 atcggccgca ccctcgtcgg cgccgcctgc acgatcgtcc tcctcatgca gggctacggc
     2581 ctcatcgccc ttgccctggt caccctgctg accaacttcg gctccctcct gctcaaacgc
     2641 ttcttccagc accgcatcgt cccccagatc gagtacaacc ggaaatacgt gaaccgccag
     2701 cgcatcaggg agctgttcaa ctacggcctc gtcagtttcc tcatcgtcat ggcctggatc
     2761 gtcatcttct ccaccgacaa catcgtggtc ggcatgttca tctccaccca ggcggtcgcc
     2821 tacttcaaca tcgccggcac catcatcaac aacctccgct ccatcgtggc ctcgatcggc
     2881 acccctttgg tcccggtgat cagccacctc gacgccacca gcgacctggc cgagatcgcc
     2941 gccctccacc gcaagaccgt gcgctacctc tactacatct ccactctcat cggcgtggga
     3001 gtgttcttct tcgggcgcca gttcgtcggg ttgtggatgg gccccggctt cgacaccacc
     3061 gtcaccatcc tcttcatcct catcgccccg gcctgcatct atctgccgca gatgatcacc
     3121 aactcggtgc tcctcggcat cggccgtcac cccacgctgc tgtacatcct gggggtcgag
     3181 gccctcgcca acatcggtct gagcctcgcc ctcgtccacg tgtggggggt ctacggcgtc
     3241 gcgctcggca cggccattcc acaggtcgtc atctactcct tcgtctaccc gtacgtcttc
     3301 caccgcacta tccgcgcccg gctctcggac ttctaccgcg acagcggcct ttccatcgcc
     3361 actgccctcc tgttcacgct gccggtcgcc tgggcggtgt cctacctgcc gctgcccggc
     3421 tgggtgggat ttttggcgca gatttcggct gtcaccgccg tctcgctggc cggcttcgcc
     3481 tggcggatcc ttgatgacgc tgaccgccgc cgcttcctcg gccggttttt ccgctcgcgg
     3541 gcggctggac ctcaaccgcc ggagaaaaag accgccccgg gccgctgagc ccgcgcggga
     3601 aatcccatgg gactgtacaa cctcttccac agcaccctct tccggctgaa agacctccgc
     3661 gatcgcaccg gcgtgtaccg cctggcggcc cggctgcgcc gcgagcagtt cctccctcga
     3721 ccggacctcg acgatctccg ccgccgccgg ctcctcgacc tgctggcgca cgcccgtctc
     3781 cattccccct actaccgcga cctcctcgcc ggcctcgaac ccgatctgga gcgaattgcc
     3841 ccgcacgaaa tcctctcgcg gatccctctg ctcacccgcg agattgttca ggaggagggc
     3901 tcccgcctcc tctgccccgg catcccgggc tcctacccgg attcctctgg cggctccacc
     3961 ggccggccgg tcaatttcca ccacgacctg acctacctca actggggccg cgcccatgag
     4021 ctgttctttc tctcgtggat gggggtccgc cccggcgacc gcacggccgt cttctggggc
     4081 gccgaccgcg acctcaagga ggagtccctg tacacgcgtc tcatgctccg cctcaaccgt
     4141 gttcgccagc tcaactcctt tgcgatgtcc gaggcccaga tcgacgcctt tctcaaacag
     4201 ctcaacgctt tccgaccgcg ctacatctac ggctacgcct cctccctgta ccacgtcgcc
     4261 actttcatcg cccggggccg ccccctcggc ttccgccccc tcgccatccg gtcttccgcg
     4321 gaaatgctct accccgtcca gcgcgccgcc atcgagcagg cgtttgacgc ccccgtgcac
     4381 aatttctacg gctcgcgcga ggtcaacaac atcgcagccg aatgcgccgc ccacgacggt
     4441 ctgcacatct tcgcctcggc ccgcatggtc gagattgtcg acgaccgcgg tcgcctcctg
     4501 ccccccggcg aggtcggcca catcgcggtc accgatctga ccaaccgcgc gttccccttc
     4561 atccgctacc tcaacggcga tctggcggcc ctgaaatcag gcctctgccc ctgcgggcgc
     4621 acctaccccc ggttggagaa gatcgccggc cgaagctccg acatcattac agttgagggc
     4681 cgccatatcc atggcgagta cttcacccat ctcttttatg gccgccctga agtcaaagcc
     4741 tttcaattga ttcaggaaac ggaatacgac tttcgactcc tcctggtccc gcgcactcgg
     4801 gacctcgaca ccgccttctt ccgcgaccgc atccgcgaca agctcggtcc ccgcgtccgg
     4861 cttgaaatcg ctctcgtcga cgatatcccc ccgaccgcgg gcggcaagca ccgcttcacc
     4921 gtctccaaag tcccgccccg caaggacggt ctttgaaact gcattagaac ggcctatatt
     4981 gaaggtcgga acgaaaacgt ccgaacagac ggtataagcg actatggcaa ccacaacgca
     5041 tcatctcccc ggcggatcgc gggcgtacct gcggcccgag catctcgcct acgccgcttt
     5101 cgccgccgcc atgatcctct cgggcctgct ggtcgccctc cccttcccct accacatcct
     5161 cccgtttgcg ctcctcggcg tcgtcgtgtt cgtcatcggc accctgcgct acccctactt
     5221 cggcctctac ctctacctca tcattttctt catggacccc aaccagatct tccccttcat
     5281 gcagacggtc gcgtttcctt acgagaaagt tctcgccttc gccgtcatcg gcatcctcct
     5341 catccacatc gctattctga agaaggaatt cgacctgtac cggctcgacg tcggttttgc
     5401 ggcgatgatc ctcgccacct acctctccat cctcggcgcc gtcgatctcg ccgccgcctg
     5461 ggaggactgg cagcgcttcc tccgcctgtt tctggtctac ctggtcatgg tcaggatagt
     5521 caagacggag gggcagctca aagccatcat catgctcttc atcatctcca tcgggttttt
     5581 ggccatctcg tccaccatcc agtactaccg cggcgagttt gaggtgcgcc agggtatcca
     5641 gcgtctccac tctttcggcg gcgggctcat cgaccccaac accatggcct cgagcctcgt
     5701 cctcggcgtt cccttcatgt tctacatggc caaggcccac cgcagcgcgc tgttgaagct
     5761 gttcatgttc gccctcatcg gctcctgttt ctgggccatc atgctcaccg gatcgcgcgg
     5821 cggcatggtc ggtggcatcg ccctgatgct cctcctcgtc tggcactcgc gccacaaaac
     5881 gctcggcttc gtcgtcgcct tcgccgttct ggtctgcatc gccgccgtca tgcccgatca
     5941 gtatcagaac cgcttcatgt ccattttccg catcaacgag caggatgaat tcggcgcttc
     6001 cgattcggcc cgcgggcgca tcaacggctt caaactcggc ttcaaattcc tcctgaagaa
     6061 tcccctgacc ggcgtcggca tctccaattt cggctggcat caccacctcg agccgaacgg
     6121 cgactggacc gacgcccaca acctggtcgg caaactcgtc ggcgaactcg gcctcatcgg
     6181 cgtcgccgcc ttcagcttct tcatcgcccg cttcgccggc acgctcaaac tcatcaaacg
     6241 cctgtacgac gaacacggct ggccgcccga cttccttttc cagatgcgcc aggccatcaa
     6301 gatcggcctc attatgctct tcgtgcaggg cctgtttggc cacaatcttt tccgcgacaa
     6361 ctggtacttc acggccggct tcatcgtcat cgtcagcaaa atcgtctggg accgccacct
     6421 cgccgcccag cgcccgtcag gcgccacccc cggctccccc gaacccctcc ccggtgcgga
     6481 gaaaggactc ctgcccggat gagacttacg cgctttcccg cccgcccgcg cggccccctt
     6541 gaactgttcg ccgccctcaa ccgctacgcc ctcgccgcct atcggggcga gcgcgccatc
     6601 tacccccttg tccgcgagct cgagcgcaac ttctccctct cccgcgatga gctcgaggcc
     6661 gcccaggaca aggcgctcgc cgcgatcgtc gaccacgcct tcgcccacag cccgttctac
     6721 cggcgcaaat tcgcggccgt cgggttcaaa ccgggcgacg tcaagggccg ggccgacctc
     6781 gccctgctcc ccgagaccaa caagatcgag ctcgtccgcg ccgcagacga ggtcctctgt
     6841 ttgccgaaag accgcctccg cgccgctggc accggcggga cctccggcat ccccttcacc
     6901 ttctaccgcg acccgcgctg tctcctcttc cgccgcgccg tcgattacac caccttccgc
     6961 tactacggct ggcgcgcggg gcagtggcag ggatggctct ggggcgcccc tcgcgacctc
     7021 gtggcaccgg cgaccgcgcg gggaagactc ctccagcgct gggccgtgcg cacgtttttc
     7081 gccgacgtca cccgcctcgg gaccgagagc taccgcagct tcgccgccct cgcccgccgg
     7141 taccgcccga cctggctttc cgcctacccg tcgctcgcct acgacctcgc cgccgccatc
     7201 gaggccggag acatcgagcc gttccgcgtg ccgatggtca acctcacggc cgaaccggtc
     7261 tacgacttcc agaaacaact cattcgggcg acctttggcg aggaggtctg cgagcgctac
     7321 ggatcgcgcg agatgggtct ggccgccctc gagtgccccg cccacaacgg cctccactat
     7381 tttatggaaa gcgtgtactt cgaaagcatc gatcgcggac ccggcagtcc cggccgcagc
     7441 ctggtcgtca ccgatctggt caaccgcggc atgcccttta tccgctatga caccggcgac
     7501 ttcgcccggc tcgacgacgc tccctgcacc tgcggccgca caaccccccg cctcattgac
     7561 atccgcggcc gcgagaccga caacatctgg tgtcccgatg gccacggcat cgccggcgcc
     7621 attgtcgtct acctcttcgc ccggatcaac cccggcgtgc ccatgcagct catgcaggag
     7681 tcgctcgacc gcatcgttat ccgcatcgcc ggccggcccg gggaactcgc cgggcgggcc
     7741 gccgaactca tcgacgtctg ccgacgtgag atcagccccg acatcgccta caccatcgaa
     7801 tacgtcgacc atatcgagcg cgccccctcc ggcaagtacc gctacattat ctcgcgcgtg
     7861 ccgcccccct cccgccgccg ctgagtccgc cccgaccggc gcgctccccg gcggccaaga
     7921 accgtaaaat tggaccggac tttccgtttg aatttgagta tgttgaaacg atcatgcagg
     7981 cgccgtccgg atccccggtg tgcactcggg ccccggtgag cgcggcgccc gccggaaggt
     8041 agctatggat gagaccacgt cgcgcctccg cctcttgctg attgacgagg aattccccta
     8101 ccccctcact tcgggcaagc gcctccgcac ctacaacctc ctccgcgaac tgtcccgccg
     8161 ctgcgacatc acgacgctcg cctacggcga tcctgactcc gagggtttcc gccggttcga
     8221 acgcgacggt ctcaccccgg tggctgtccc ggccccggac cgtcgccaga gcggcctccg
     8281 cttctactgg cgcctcttcg gcaatctctt ctccccctat ccgtacatcg tcaccagcca
     8341 caccacccgc cgtttccacc gccgcctccg ccagctcctc gcccggcact cgttcgacct
     8401 ggtcctgtgc gagtggaccc cctacgcccg gtttgtcaag gacctgccgg gcgtccgaaa
     8461 agtgatcacc gcccacaaca tcgaagccgc tatctggcgc cgctacgaac agcaggaaac
     8521 ccagccgctc cgaaggctgt acattcggct tcagcgcacc aaggtggaag cgttcgaggc
     8581 gcgctgtttc gggtgggtcg acggcgccat cgcggtctcc gagagcgaag cccgccagat
     8641 cgccgagctc ggcgtcccct accccccggc ggtggttgag aacggcgtcg atgtcggtta
     8701 tttcaccgcc ccggccgacg accccgaccc cggtcaaatt gtcttctccg gcgctatgga
     8761 ctggcgcccc aatcaggacg ccgcgctctg gtttgtccag gagatctttc cgctcctccg
     8821 ccgggcgcgc cccaacctcg ccgtcaccat cgtcggccgc gatcctgtcc gccgcatccg
     8881 ggaactcggg cgcgtcgagg gcgttacggt caccggcacg gtcgccgatg tccgtcccta
     8941 cctcgcccgg gccgccgtct tcattgtccc cctgcgcatc ggcggcggct cgcgcctgaa
     9001 gatcctcgag gccatggcca tgcgcaaggc ggttgtctcc accagcgtgg gcgccgaggg
     9061 ccttcgggtg gccgcggacg aaaacatcat gatcgccgac gacgctccgg cgttcgcgca
     9121 ggccgttctg cggtgcctgg gcgaacccga cctgcgcagg cgactcggcg aaaacggccg
     9181 ccgcctcgtc gaacgggagt accgatggga tattctcgga cacaaaatgt acgagtactt
     9241 atgccgcgtg atgcagcaaa agtgagcgtt ctccacctgc gggcgagcaa cttctacggc
     9301 ggtccggaac gccagctcca ttgccacgcc cgggccgccc gcgactccgt cttcgaggtc
     9361 atcgtcgcct cctacgccga gtccggtcgc tcccccgacc tgctggcccg ggtggccgcc
     9421 gaccgtctcc cgactcacct cgtcggcgtc gccggcgcct acgaccgccg cgccctcggc
     9481 ctcatccgcc gggtcctgcg cgatcgcgac atcgccatcc tctgcaccca cgactaccgc
     9541 tcaaccgtcc tcggctgcct cgccgtccgc ttcacccccg tccgctggat cgccttctcc
     9601 cgcggccgca cgcgcgagac cctcaggatt cggctctaca acgaactgga gcgcttgtgc
     9661 ctgcggttcg ccgaccgcgt cgtcgccgtc tccgagggcg agaaagcctc cctccgccgc
     9721 cgtctcgtgc ccgaccgccg catcgccgtc gtccacaacg ccatcgacac cgccgctttc
     9781 gccgccgtca cccccgtcga cctgaaaagc cgcttcggct tccctgcgca ctctctcgtt
     9841 gccgtcgccg ccggccgctt ctcgcctgaa aag
//
LOCUS       DAOPOE010000102         9814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7852_length_9814_cov_6.858285, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000102 DAOPOE010000000
VERSION     DAOPOE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_9814_cov_6.858285"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1276)
                     /locus_tag="PKM94_11545"
     CDS             complement(95..1276)
                     /locus_tag="PKM94_11545"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOD67309.1"
                     /translation="MSTALSPNDIRNYEFPNQMRGYDKDEVRELLQQVAAALEDLKQE
                     NLKIAIERDTVRTQLEALKQFEDTIKSAAIDARRNADTLVANAKREVGEILNKARAEA
                     ETIVGSRADEARVLDEQITRLRMVKVSYQTKLKNLIESHLELIDELQRVKEPEVPRMQ
                     PARPTAAAEPSADDTAKTPTITSIPATPAASETEPARAAGDFRRDLRTATTAPEADWI
                     RQAREANIPAVVPPTLRPFKPTNGLEVEEVTEVTRKKLETFSTEPEPAEPAHTEEANA
                     AEQIVAVGPAPTAPPPPQPAIDPELAAALESYQMSHATAPELSRRTAAPAAGPTAAAA
                     SERDESMRATLNDFDERNRTGRIPAPETPEEADASTEHNLIDIDQPIGDAALIGLHSG
                     Q"
     gene            complement(1497..2234)
                     /locus_tag="PKM94_11550"
     CDS             complement(1497..2234)
                     /locus_tag="PKM94_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOD67310.1"
                     /translation="MKADIGRNIVELRGRIVEACEQYDRDADDITVVAITKTHPPSVI
                     RTAVAAGLHNIGESRVQEADAKIAALGQIARFHMVGHLQTNKVKRAVELFDVIQSVDT
                     LKLADEINRQAGKAELEIECLVQVNCSGEDQKYGVSPDACLDLCRQIRNLENIVLSGL
                     MTIGPLADDEDSVRAAFRRCRELFRQGQDLFGDEFDTLSMGMSGDFPLAIAEGSTMLR
                     IGSLLFGQRPTDEFSYTEDRESDKPSD"
     gene            complement(2231..3130)
                     /locus_tag="PKM94_11555"
     CDS             complement(2231..3130)
                     /locus_tag="PKM94_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67311.1"
                     /translation="MTARTQRAPDPCADTSFHPADCPGPGRYFQYPLYPDSSCYFTPH
                     GFNAVPLRDSTEPPSSSLTRIRKTYPDLPDPRTLPPDSLTSDMYTEDGYLKFYEYAGD
                     IPGLLPSVPYWVAVTAFDFGSPESCVRPLESAIAETAVSGFPLHAFDAPLSGSNQVYI
                     YPNPYRTDAGYRVQGFEGRAQEDRWDERVRAIWFANLPPQCTIHIYTLNGDRVRTLDH
                     DTNPADPLHTRHAWDLINRNFQKVESGLYYWVVEVPGQPAQIGKLAIIREPRLSAARE
                     SPDSRRAFCLPESPFPFILAPVP"
     gene            complement(3264..3854)
                     /locus_tag="PKM94_11560"
     CDS             complement(3264..3854)
                     /locus_tag="PKM94_11560"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD67312.1"
                     /translation="MIRFLAPLLAFGLLLAGCGDNSADTRPRAERTPQTAAAQPPART
                     QPPGQVAFTLRDTDGNIRTADEWLGRKPVVINFWGTWCGPCRREIPDMVKIYREYSDR
                     IEILGIALNDTPDKVKRFSAQQGMTWQMLMGDQTVAVDFGIRGIPTTFFFDAKGNLFQ
                     VEDYNGTIVNHYVGPRDYKTFKRAIESIINSSPAGS"
     gene            complement(3961..4479)
                     /locus_tag="PKM94_11565"
     CDS             complement(3961..4479)
                     /locus_tag="PKM94_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOD67313.1"
                     /translation="MKAITPIMLPLVLVLAAGAFVFADDESGKTAAPAEIQWVAYDVG
                     LQRAAAEKKHVLVDFTAKWCGFCKKMDRETFAKPEVVEMINANFIPVKVDGDSQKELN
                     VNGYKITEANLAKKEFGVRGYPTFWFLKSDGSKLGQITGYRPPDVMMEALSYVKDEQY
                     DTTKTETPKSND"
     gene            complement(4505..7948)
                     /locus_tag="PKM94_11570"
     CDS             complement(4505..7948)
                     /locus_tag="PKM94_11570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD67314.1"
                     /translation="MFANFVHLHTHSQYSLLDGACRLDDVIALAREQRMPALAITDHG
                     NMFGAIEFYTKARRAGLKPIIGCEAYVAGGSRLDKKPSSRWPDGGFHLVLLVRNLTGY
                     QNLVKLASAAFLEGFYHRPRIDKELLRRHSEGLIATSACLKGEVAWHLLQGRTDEAVA
                     AARELQDIFGEGNFFLEIQNHGLEQELQNIPRMEAISRETGIPLVATNDCHYLRREDA
                     EAHDALLCIQTGKMVEDTQRMRYRTDQLYFKSAEEMEASFGDFKEALRNTIRIAEACN
                     LELEMDRLLLPVFPIPAAFSNPDDCLEHLCREGCRERYGRLSDEIEGRLRYELGVIRQ
                     MGYAGYFLIVKDFCDYARREGIPVGPGRGSAAGSLVSYALRITNIDPLAFGLLFERFL
                     NPERVSMPDIDIDFADRGRDKIIKYVIDKYGEDNVCQIITFGTLAARGVIRDIGRVLG
                     MPYGEVDRIAKLVPEGPGQTIELALKKVPELAELRKKDERVDRILSYAVTLHGLARHC
                     STHAAGVVIAPSALTSYVPLFKSGKDEITTQYDMKMVEKIGLLKMDFLGLRTLTVIDD
                     AVRMIRENDPGAALDLDNLSLDDPEVFRLFARGDTVGIFQFESPGMRDYLRKLNPSKF
                     TDLVVMNALYRPGPLDSGMIDIYIDRKHGRQRIEFVHPSLEEILGDTYGVIVFQEQVL
                     KIANVLAGYPLGRADLLRKAMGKKDAALMAAQKQEFLEGADRLGVPRSVSEEVFHQIE
                     TFARYGFNKAHSTCYAYVAYQTAWLKQYYPKEFMAALMTSEINDSDRIYAFLEECRRA
                     NIDVLPPDVNHSQVDFSVAEGKIRFGLQAVKNVGGSPALAIVEERRRDGLYSDLADLV
                     TRVPAKTLNRRVLESLVAAGAADSLPGNRAQKYAAVEAVLEFAHKVQEAAASHDLFAD
                     SAGARRVPPALPPLEEWPRAERLNQEKTMLGFWISGHPLDAYRDELKSFTTFTADGLR
                     QAADGREVTVGGILTAVKKMVDKKGNMMAFATLEDYTGSTELILFSKCYDENKEIIAA
                     DRMVLVTGRVSTREGEAPKIIGGEVLPLEKLTERFSCQLVIKIDVDCPDTVLDQTLRT
                     LDASRGSTPVLVAARENGSEVYIRSRKYAVNPDFALLNSLKAILGESGAYLRPLQRKE
                     S"
     gene            complement(8236..8859)
                     /locus_tag="PKM94_11575"
     CDS             complement(8236..8859)
                     /locus_tag="PKM94_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOD67315.1"
                     /translation="MKHVVLALALVLLLSPLASALSFSGSLASPAGVTASGYWAQQGF
                     KIEWMIEDQPDGSWFYQYWLTDAQGNPWEVAAVSHFTLEISPDATASDFWGYSGAVEF
                     GDLNGMSSAMKLDFTATHYKFYSNRAPVWGDFYAKDGRAGGLGLNEAYNAGFGAPDPT
                     AMPGNGSIGNKILRPDTSTTVVPEPGTMLLMGIGLAGAGVVRRWRRT"
     gene            9450..>9814
                     /locus_tag="PKM94_11580"
     CDS             9450..>9814
                     /locus_tag="PKM94_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOD67316.1"
                     /translation="MKGKDKAAGSQAPDRKKALEAALIQIERSFGKGAIMRLGDESVQ
                     KVQVISTGSIGVDHALGVGGIPRGRVVEIFGPEASGKTTMALHMIAEAQKAGGVAAFV
                     DAEHALDATYARNLGVDTD"
BASE COUNT         1619 a         2767 c         3578 g         1850 t
ORIGIN      
        1 ggtggaagct tcagcgcctg agccgtcggc cgcaccatcg ccgaccgcgc cgacttcgcc
       61 ccccgcgaca tccgccgcca cttccccgga aacttcactg accgctgtgc aggccgatca
      121 gcgccgcatc gccgatcggc tggtcgatgt cgatcaggtt gtgctcggtc gaggcgtccg
      181 cttcctccgg cgtctcggga gccgggatcc ggccggtgcg gttgcgctca tcgaaatcgt
      241 tgagggtcgc gcgcatcgat tcatcccgtt ccgacgccgc ggcggccgtc gggccggcgg
      301 cgggcgccgc tgtccgccgc gacagctcag gggcggtggc gtgggacatc tggtagcttt
      361 ccagggcggc ggccagttcc gggtcgatcg ccggctgcgg gggcggcggc gccgtcggag
      421 cgggaccgac cgcgacaatc tgttcggcgg cgttggcctc ttcggtgtgc gccggttcgg
      481 ccggttccgg ctccgtgctg aaagtctcga gctttttgcg cgtcacctcg gtgacttctt
      541 cgacctcaag gccgttggtc ggcttgaacg gtctcagggt gggcggcacg acggcgggga
      601 tgttggcctc gcgcgcctgc cggatccaat ccgcctccgg agcggtcgtc gccgtcctga
      661 gatcgcggcg gaaatcgccg gcggcccggg cgggctccgt ctccgaggcg gccggggtcg
      721 ccggaatgct cgtgatggtc ggagtcttcg cggtatcgtc ggccgacggc tcggcggcgg
      781 cggtcgggcg cgccggctgc atccgcggca cctccggctc cttcacccgc tggagttcgt
      841 cgatcaactc gaggtgcgac tcgatgagat tcttgagctt ggtctggtac gagaccttga
      901 ccatgcgcag gcgggtgatc tgctcgtcga ggacacgggc ttcgtcggcg cgggagccga
      961 cgatggtctc ggcctcggcg cgggctttgt tgaggatctc ccccacttcg cgcttggcgt
     1021 tggccacgag agtgtcggcg ttgcggcggg cgtcgatagc cgcgctcttg atcgtgtcct
     1081 cgaactgctt gagagcctcg agctgagtgc ggaccgtgtc ccgctcgatc gcgatcttga
     1141 ggttctcctg cttgagatcc tccagggccg cggccacctg ctgcaggagc tcgcgcactt
     1201 catccttatc gtatccccgc atctggttcg ggaactcgta attgcggata tcgttcggcg
     1261 acagggccgt actcatgggt cagctccttc agtttccctt ttccagtcca ccggcgccgg
     1321 gaacggaaag atgcctttcc ggcagccacc gggccggtac ggtctgtatc tgtattagtt
     1381 atcgggagac gggggatgaa aattgagaac ggaatgggac ttgagaaggc gggacggccg
     1441 cctgccgcag agtcggagca ggttgtatgc gcaagcagga cagagagata gacagctcag
     1501 tcggacggct tgtcggactc ccggtcctcc gtgtacgaaa attcatcggt cggacgctgc
     1561 ccgaacagaa ggctgccgat gcgcagcatg gtcgagccct cggcgatcgc cagcggaaaa
     1621 tcgcccgaca tacccatcga gagcgtgtcg aattcgtcgc cgaacagatc ctgtccctgc
     1681 cggaacaact cgcggcagcg ccggaatgcg gcgcgcacgc tgtcctcgtc gtccgcgagc
     1741 gggccgatcg tcatgaggcc ggagaggacg atgttctcca gatttcggat ctgccggcag
     1801 aggtcgagac aggcgtccgg cgaaacgccg tatttctggt cttccccgga gcagttgacc
     1861 tgaacgaggc attcgatttc gagttcggct ttgccggcct ggcggttgat ctcatcggcc
     1921 agtttcagcg tgtcgaccga ctggatgacg tcgaaaagtt cgacagccct tttgactttg
     1981 ttggtctgga gatggccgac catgtggaaa cgggcgatct ggccgagggc ggcgattttc
     2041 gcgtcggcct cctggacccg gctctcgccg atattgtgca ggccggcggc gaccgcggtg
     2101 cggatgacgg acggagggtg ggttttggtg atggcgacca cggtgatgtc gtcggcatcg
     2161 cggtcgtatt gttcgcaggc ctcgacgatt cgtccgcgca gctcaacgat atttcggcca
     2221 atgtccgctt tcatggtacc ggcgccaaga tgaagggaaa gggggattca ggcaagcaaa
     2281 aagcgcggcg ggagtccggg gactcccgcg ccgcggaaag gcggggctcg cggatgatgg
     2341 cgagtttgcc gatctgagcc ggttggccgg gaacctcgac cacccagtag tagagcccgc
     2401 tctcgacttt ctggaagttc cggttgatga gatcccacgc gtggcgggtg tggagcggat
     2461 cggcgggatt cgtgtcgtgg tcgagggtgc gcacccggtc gccattgagg gtgtagatgt
     2521 ggatggtgca ctgcgggggg aggttggcaa accagatcgc gcggacgcgc tcgtcccagc
     2581 ggtcctcctg cgcccgtccc tcgaacccct ggacgcggta gccggcgtcg gtgcggtagg
     2641 ggttggggta gatgtagacc tgattgctcc cggagagggg ggcgtcgaaa gcgtggaggg
     2701 ggaagccgct cacagcggtt tcggcgatgg ccgactccag agggcggacg catgattcgg
     2761 gggagccgaa gtcgaaagcg gtgacagcga cccagtaggg gacactgggg aggaggccgg
     2821 ggatatcacc ggcgtactcg tagaacttga ggtagccgtc ctcggtgtac atgtcggagg
     2881 tgagggagtc gggcgggagg gtgcggggat cggggaggtc gggataggtt ttccggatgc
     2941 gggtgaggga agaagaggga ggctcggtcg agtcgcgcag ggggacggcg ttgaagccgt
     3001 ggggggtgaa gtagcaggag gagtcggggt agagagggta ctgaaagtag cggcccgggc
     3061 cggggcaatc agccgggtga aagctcgtgt ctgcacaggg gtcgggagct ctttgtgtcc
     3121 tcgccgtcaa gctacgcccg cccggggcgt ttgtcaggag aatgagttga tgtgaggggt
     3181 aacccacagc agagctgtgg gcgaccagcc gggaatgatc gcgccgggcg gggcctgtga
     3241 tacgaccagc ggcggagggc gcctcaggag ccggcgggag aggaattgat gatcgactcg
     3301 atggcgcgtt tgaaggtttt gtagtcgcgg gggccgacgt agtggttgac gattgtgccg
     3361 ttgtagtcct cgacctggaa caagttaccc ttcgcgtcga agaagaaagt ggtcgggatg
     3421 ccgcggatgc cgaaatccac cgcgaccgtc tgatccccca tgagcatctg ccaggtcatg
     3481 ccctgctggg cggagaagcg tttgactttg tcgggggtgt cgttgagggc gatgccgagg
     3541 atttcgatgc ggtcgctgta ttcgcggtag attttcacca tgtcggggat ttcgcggcgg
     3601 cacgggccgc accaggtgcc ccagaaattg atgacgaccg gtttcctgcc gagccattcg
     3661 tcggcggtgc ggatgttgcc gtcggtgtcg cgcagagtga aggcgacctg gccgggcggc
     3721 tgggtccggg caggcggctg cgcggcggcc gtttggggtg tcctttcggc ccgcggccgg
     3781 gtgtcggcgc tgttgtcccc gcagccggcg aggagcaggc cgaaagcgag caggggggcg
     3841 agaaatcgga tcatggcgtc gtctcccttg ttgtccggac atgccggttg ggcggtctgc
     3901 ccgcgccccg gcgcggggcg acggagcggg cctcctccgg cagtatcggc gcggcggggg
     3961 ctagtcgtta ctcttgggag tctccgtctt ggtagtgtcg tactgctcgt ccttcacgta
     4021 cgagagggct tccatcatga cgtcgggggg acggtatccg gtgatctggc caagcttgct
     4081 gccgtcggac ttgagaaacc agaacgtggg gtagccgcgg acgccgaatt ctttcttggc
     4141 gaggttggct tcggtgatct tgtagccgtt gacgttgagt tccttctggg agtcgccgtc
     4201 gactttcacc gggatgaagt tggcgttgat catttcgacg acttcgggtt tggcgaaagt
     4261 ttcgcggtcc atcttcttgc agaagccgca ccacttggcg gtgaagtcga cgaggacgtg
     4321 ctttttctcg gcggcggcgc gctggaggcc gacgtcgtag gcgacccact ggatttcggc
     4381 cggggcggcg gttttgccgg actcgtcgtc ggcgaacaca aacgcgccgg cggcgaggac
     4441 gagcacaagc gggagcatga tgggcgtgat ggctttcatg aggcaccttg ttgcggtatg
     4501 gatgttaact ttctttgcgc tgcagggggc gcaggtacgc gccgctttcg ccgaggatgg
     4561 ctttcaggga gttcagcagg gcgaaatcgg ggttgacggc gtacttgcgc gaccggatgt
     4621 acacctccga gccgttttcg cgggccgcca cgagcaccgg ggtacttccc cggctggcgt
     4681 ccagcgtgcg gagcgtctga tcgagtacgg tatcggggca atccacatcg atctttataa
     4741 ccaactggca ggagaagcgt tccgtcagct tctccagcgg cagaacctcg ccgccgatga
     4801 tcttcggagc ctccccctcg cgcgtcgaca cccgcccggt caccagcacc atccggtcgg
     4861 ccgcgatgat ctccttgttc tcgtcgtaac atttcgagaa gagaatcagt tcggtcgaac
     4921 cggtgtagtc ttcgagggtg gcgaacgcca tcatgttccc tttcttgtcg accattttct
     4981 tgacggcggt gaggatgccc ccgaccgtca cctcgcggcc gtcggccgcc tgccggagac
     5041 cgtcggcggt gaaggtcgtg aacgacttca actcgtcgcg gtaggcgtcg aggggatggc
     5101 cggagatcca gaagccgagc atggtctttt cctggttgag gcgttcggcg cgcggccatt
     5161 cctcgagggg cggcagagcg ggggggaccc gccgcgcgcc ggcgctgtcg gcgaacaggt
     5221 cgtgggaagc ggcggcttcc tggactttat gggcgaactc gagcacggcc tcgacggccg
     5281 cgtacttctg cgcccggttg ccggggaggg aatcggccgc cccggcggcg acgagggatt
     5341 ccaggacgcg gcggttgagc gtcttggccg gcacccgggt caccaggtcg gccaggtcgc
     5401 tgtagaggcc gtcccgccgg cgttcctcga caatcgccag cgccggcgag ccgcccacgt
     5461 tcttcaccgc ctggaggccg aaccggatct tcccctcggc tacggaaaaa tcgacctggg
     5521 aatggttgac gtcgggcggg agcacgtcga tgttcgcgcg gcggcactct tcgaggaagg
     5581 cgtagatgcg gtcggagtcg ttgatttccg aggtcatgag ggccgccatg aactctttcg
     5641 ggtagtactg cttgagccag gcggtctggt aggcgacgta ggcgtagcag gtggagtggg
     5701 ccttgttgaa gccgtagcgg gcgaaggtct cgatctggtg aaagacctcc tcggagacgc
     5761 tgcgcgggac gcccaggcgg tcggccccct cgaggaactc ctgtttctgg gcggccatga
     5821 gagcggcgtc cttcttcccc atcgccttgc gcagcaggtc ggcgcgcccg agcgggtagc
     5881 cggcgaggac gttggcgatc ttgagcacct gctcctggaa aacgatcacg ccgtaggtat
     5941 cgccgagaat ttcctcgagc gacgggtgga cgaattcgat cctctgccgg ccgtgcttgc
     6001 ggtcgatgta gatgtcgatc atgcccgagt cgagcggacc ggggcggtag agggcgttca
     6061 tcacgaccag gtcggtaaac ttcgacgggt tgagcttgcg gaggtagtcg cgcataccgg
     6121 gggactcgaa ctggaagatg ccgaccgtgt caccgcgggc gaagaggcgg aacacctcgg
     6181 ggtcgtcgag cgagaggttg tcgagatcga gggccgcgcc ggggtcgttt tcccgaatca
     6241 tgcgcacggc gtcgtcgatg acggtcaggg tgcgcaggcc gagaaagtcc atcttcagca
     6301 ggccgatctt ctcgaccatc ttcatgtcgt actgggttgt gatctcgtcc ttgccggact
     6361 tgaagagggg gacgtagctg gtgagggcgg agggggcgat gacgaccccg gcggcatggg
     6421 tggagcagtg gcgggccagg ccgtggaggg tgaccgcgta ggagaggatg cggtcgacgc
     6481 gctcgtcttt cttgcgcagc tcggccagct cggggacttt cttcagcgcc agctcgatcg
     6541 tctgcccggg gccctccggg acgagcttgg cgattctgtc gacctcgccg tagggcatgc
     6601 cgaggacgcg gccgatgtcg cggatgacgc cgcgggcggc gagggtgccg aaagtgatga
     6661 tctggcagac gttgtcctcg ccgtacttgt cgatgacgta cttgatgatc ttgtcgcggc
     6721 cgcggtcggc gaagtcgatg tcgatgtcgg gcatggacac gcgctcgggg ttgaggaagc
     6781 gttcgaagag aaggccgaaa gcgagcgggt cgatgttggt gatgcggaga gcgtaggaga
     6841 cgagcgaccc ggcggccgag ccgcggccgg gaccgaccgg gatcccctcg cggcgggcgt
     6901 agtcgcagaa atccttgacg atgagaaagt agccggcata gcccatctgc cggatcaccc
     6961 ccagctcgta gcggagacgc ccctcgatct cgtcgctcag ccggccgtag cgctcccggc
     7021 agccctcccg gcagaggtgc tccaggcagt cgtcggggtt ggagaaggcg gcgggaatcg
     7081 gaaagaccgg caggaggagg cggtccatct cgagttcgag gttgcaggcc tcggcgatgc
     7141 ggatggtgtt gcggagggcc tccttgaaat cgccgaacga ggcctccatc tcctcggccg
     7201 acttgaaata gagctggtcg gtgcggtagc gcatgcgctg ggtgtcctcg accatctttc
     7261 cggtctgaat gcacaggagc gcgtcgtgcg cctcggcgtc ctcgcggcgg aggtagtggc
     7321 agtcgttggt ggcgacgagc gggattccgg tttcgcgtga gatcgcctcc atgcggggaa
     7381 tgttctgcag ctcctgctcg agaccgtggt tctggatttc gaggaagaag ttcccttcgc
     7441 cgaaaatatc ctgcagctcg cgggcggcgg cgaccgcttc atcggtccgc ccctggagga
     7501 ggtgccaggc gacctcccct ttgaggcagg cgctggtggc gatcagccct tcgctgtggc
     7561 gacggaggag ttccttgtcg atgcgggggc ggtggtagaa gccttcgagg aaggcggccg
     7621 aagcgagttt gacgaggttc tggtagccgg tgaggtttct cacgaggagg accaggtgga
     7681 aaccgccgtc gggccagcgg ctggaaggtt tcttgtcgag gcggctgccg ccggcgacgt
     7741 aggcttcgca gccgatgatc ggtttgaggc cggcgcggcg ggccttggtg tagaattcga
     7801 tcgccccgaa catgttgccg tggtcggtaa tagcgagcgc cggcatgcgc tgctcgcggg
     7861 ccagcgcgat cacatcgtca agacggcacg ccccgtccag cagcgagtac tggctgtgcg
     7921 tgtggagatg gacaaaattc gcaaacatag gctcaggcca atcaaccctc ccctcttccc
     7981 tgcttgtacg gcggccggtg tcttcggatc gcggaaaggt gacggccggg cggtacgggt
     8041 cgggtatgac aagggcggtc cccgccgggg cggtcgccgc cgtccacaga tacaaaaaaa
     8101 caggcgctgc ggggtttcgt caaacgattt gtgaagggcg ggcggcgggc gggcggcggg
     8161 agggcgcggg gccggacgac gaaaggcggt ccggtcggac cgcctttcgg atttggggag
     8221 atgttactct cgtgcctagg tgcggcgcca ccggcgcacg acacccgcgc cggccaggcc
     8281 gatgcccatg agcagcattg tccccggctc cggcaccacc gtggtggagg tgtcgggacg
     8341 gaggattttg ttgccgatgg agccgttgcc gggcatggcg gtgggatcgg gggcgccgaa
     8401 cccggcattg tatgcctcgt tcagccccag accgccggcc cgtccgtctt tggcgtagaa
     8461 gtcgccccag acgggagcgc ggttggagta gaacttgtag tgagtggccg tgaagtcaag
     8521 cttcatggcg ctgctcatgc cgttcagatc gccgaactca acggcgccgg aatagcccca
     8581 gaaatcggac gccgtagcat cgggagaaat ctccagcgtg aaatggctga cagccgcaac
     8641 ctcccaggga ttcccctggg cgtcggtcag ccagtactgg taaaaccagg aaccatcggg
     8701 ctggtcttcg atcatccact caatcttgaa gccctgctgc gcccaatagc ccgaggcagt
     8761 gacgccggcg ggggacgcaa gggagccgga gaatgagagg gcgcttgcca gaggcgacag
     8821 caacaagacc agcgccagtg caagcacaac gtgtttcatc ggcaactcct catgcagtat
     8881 agggttaatg ggtgttcggt attcctgccg gcggtctgtg cggatcaatg cacatctcac
     8941 cggttgtttg ccctgccgtt tggtctgagg gtctccacaa cagcgcgatt gtctcccagc
     9001 ggggccgcga cgacaagcgc taaactgttg ttttcatttc cagtcaattc acgggttcgc
     9061 agtcgtccgg ttccttcgcc cgaccgaggg tcgggccgac agagcccggg tggtggtcat
     9121 gccgggggat tagagcaaca agtatgcccc ggcgggggaa atcgaaagca ggcgccctct
     9181 tgtattttat tgtccgacaa caggttgaat ggtgcggaag gagtgctgtc ggaaagttcc
     9241 gggcggggtg tgaagcaggg catgcaaact tccgcagaac gaccgatcgc cccctggacc
     9301 ggaccggtga aaccgctgcg gtggagacgg ccaggggcgg acaattcggt ttccggagag
     9361 tgaaagatgt gatatatttc cgaaaacgtt cgttctataa tggcgggaga agcgcacatt
     9421 taggcaggca gagaaggagc cggatggtaa tgaaaggcaa agacaaggcg gcgggatcgc
     9481 aggcgccgga ccgtaagaaa gcgctcgagg cggcgttgat tcagattgaa cggtcgttcg
     9541 gcaaaggggc gatcatgcgg ctgggggacg agtcggtgca gaaggtgcag gtgatctcga
     9601 ccggctcgat cggggtcgat catgcgctgg gggtcggggg gatcccgcgc ggccgggtgg
     9661 tcgagatatt cggcccggag gcgtcgggaa agacgaccat ggcgctgcac atgatcgccg
     9721 aggcgcagaa agcgggcggg gtggcggctt ttgtcgatgc cgagcacgcg ctcgatgcca
     9781 cgtacgcgcg caatctcggg gtggataccg acaa
//
LOCUS       DAOPOE010000103         9743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7913_length_9743_cov_5.541185, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000103 DAOPOE010000000
VERSION     DAOPOE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7913_length_9743_cov_5.541185"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PKM94_11585"
     CDS             complement(<1..1386)
                     /locus_tag="PKM94_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67317.1"
                     /translation="MSTRPHRCQSRFAFTDTATAVLIIIFALAFIAAGRAGGLGLWGL
                     NHLRYLPPAFEALAWLGLIFHLLCLHPRYAPVPALIAAWGHELFFGRLAWQWRLGLAA
                     LAGAAFWFFQAETHFLGDGLTWTANFSQAAGHVRKWAEFGSIPLVRTLQGLLGGYTRG
                     TAEAAFRAISIAAGIAALLGLFPLVALLVKHPVHRVAALATLLFSGAGLLYFGYVEFY
                     PLLWAAAVWFFRLALVWLQEHRAGALAGALLVYAAALCIHVQAVWLAGAAAALVPGVV
                     AARTGELSRRRSRLLLATLGLGALAAYLWFLFGPTDRNWIVLPPWSGRIEAPEYAVFS
                     LKHLGDLFNLALVLFPGAVVLAAARLRPAGLAAEPIAVFLAAASLGGLCFALCLNPVI
                     GMGRDWDLLSLAGLPAVLWLFHRLERVGPPLTRRVGVELGVLAMMTGVTYWAANLRVE
                     ASEERFLSLLRW"
     gene            complement(1444..2904)
                     /locus_tag="PKM94_11590"
     CDS             complement(1444..2904)
                     /locus_tag="PKM94_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOD67318.1"
                     /translation="MKALFRILALLAVLLLLPWAIKAAGVLPLGSPEFEFLYDRLERA
                     DALTLDTADRQLGPYPLALAREALGPFAWLDRLDRSDLRVEGRLGEDFRSAKGHPTVG
                     YESFSGALAARPHDRIFVYGSFRLDEALADDPAYTGKKWRGLAGDIDEAFVHLSAGAF
                     TAALGRYASFWGPRRSLVLAPHAALDGFGYAYRWGRLVLSYRLARLDSLDPDADSAVP
                     VENRYFAGHRLDIHLSRRLRIGFFETAVFGGAGRQVELYYLNPILFYHASQLNDGVDD
                     NTFLGVDFAYEPGRGVQLYGQLLVDDYQVDRASQSDQEPNEIGLLAGAYVAEFLPRLD
                     LRAEYTRVTNRTFNQSHPRNRYTFESELLGAALGNDYDLLDLLIRRWCGEDLAAGVRL
                     AYRRQGEGRVNAAWDTPWLEVEGDYREPFPTGVVEKTFTAALQLRGLLFGHIFVDASF
                     GADRVADFGHVRGDRRTISFAAIKLITFASQALDID"
     gene            complement(2907..4316)
                     /locus_tag="PKM94_11595"
     CDS             complement(2907..4316)
                     /locus_tag="PKM94_11595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD67319.1"
                     /translation="MHLTHRRRQFLLRLAGSPGSHAGAVAALCATLMAAGSSLGLLVE
                     IAAAITLARLLAFVHPHPRPVLDAPPVRKYAGAALAAEARFAVLVLAAAYLLDWPLPR
                     LAVPVFLGLNFVTQLGLLVGARLILRRLARSPLPDRLRRARNRLLVVGTGARAQALAD
                     MILNAPELDAAIDGFLDYRRTGFWRYRDIPLLGHPDLLTPLAARVQVDAVILAVEPED
                     LSRTAPLLTAAEQLGVAVALLPDVYPTTIARMVPATLNGSPAVVWRRVPESPAALFVK
                     NALDRLGGLVGLILTLPLMLVTALAIKLESPGPVFFAQRRAGANGRPFSLYKFRTMRA
                     DAEKHKESLRGLNEMSGPVFKIRNDPRLTRVGRFLRRFSIDEIPQFYNVLRGDMSLVG
                     PRPPLPSEVARFEPWQRRKLSVKPGLTCIWQVSGRNKIDFEDWMKLDLQYIDNWSLWL
                     DAKILARTVPTVLKGDGAV"
     gene            complement(4505..5536)
                     /locus_tag="PKM94_11600"
     CDS             complement(4505..5536)
                     /locus_tag="PKM94_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD67320.1"
                     /translation="MNTLQDTPTTDRSDLLEAIAQRTMTSPSMVHPFAAAGPFLAGLL
                     GALYRPGCRLQAIGHVTPEVELAADRAEVEVIEHIGPSPFSGSLQTALQGTVLPHDIL
                     CAANPNRVTGANYSLADVQRMAETVPEGAVLVDEHYHDYFGITALPLLSDCPNVIILR
                     SFAPGGALGGDEPGYALACPRTIARLRAALPESVLPAAEARLLIDRWPGGEALAARLR
                     EGHQESLRLAEGLTRLGVQCRLSATDFILLRVADPARVGNHLSRARIAIDNLDGYPAM
                     QHYLRYRVQSPLSNDRLLQAFQAMPPESFRMKTIDGRVMTMRLSAQAARKGAGRSGNL
                     LMAALRDTE"
     gene            complement(5967..8312)
                     /locus_tag="PKM94_11605"
     CDS             complement(5967..8312)
                     /locus_tag="PKM94_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOD67321.1"
                     /translation="MRKLIIFILLVIVLVSINWFRRAMAQDLGSLSTEDIARLRRQYE
                     QKSAPAPQPEPGYYESPRIFEEDTASPGPPAAPVAAPSPPAPVRTGFERDRTLEPFED
                     LRPFGMELFENAGETNPPADLVSSDDYVLGPGDNVIVHLWGRVEHEYNLTLDREGKVF
                     IPKVGDLIAWGLTLEQMRQRMKERLAAIYSDFELTASLGKIRSIRIYVTGEVRRPGAY
                     TVSSLTSVLNAVFTAGGPNERGTMRAIAVTRGGAAAAAIDLYDLLLKGHNASDIRLQT
                     GDVVFVPVTGPRVAVRGEIRRPAIYELSGGENVSDLLRLAGGPTAAAYLDRVMLERIS
                     PAGEWLVRDLNLNCGPAQPCDTIALADGDRVTVYSIYDLRTNMVAVGGRVKHPGCYER
                     TDSTRVSDLIARGQLQPLDVYFERADVFRRYPDRRVEVIPIDLTRALAGDPASDIVLA
                     DRDSLHVYGIDDVRREQFVYIEGEVHKPGKYPLYDGMTVGDLIFLAGSFLRGADAGEA
                     ELARLDSLGNVAILPVSLSDSGGYRRLLCEDDHLFIRRIPEWDANRSVVVSGEVMYPG
                     TYFLEGRHEGLHHLLGRAGGFTANAFPTGIIFQRRSIRDNLQRANVPALLEQSQPIVL
                     DSNGVPTTDSFFSYRDESMDRIIIDMERILATGGSEGDVVMEPGDRVFIPPTPSGISV
                     LGAVGANGTLKYVEGRRVKDYIERAGNFTRRADKKETRLIRAGGEVFAGGDVMGKAVR
                     LGDIIVVPSQIKHDRDWLKTVTTVVTATAGLVTSAYLVSKL"
     gene            8923..>9743
                     /locus_tag="PKM94_11610"
     CDS             8923..>9743
                     /locus_tag="PKM94_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67322.1"
                     /translation="MGIAKFSATVATVLVLLSSGISASGFENSGVGMKARGMGGAFRA
                     IADDWTAAYYNPAGYAYILDNQLGSAWAFVHHRDEFVPNTRWGGTFDRGVFDGRTNYN
                     NHEILTNPSAGILLRLPVAGETVFGLSAYQRFDRNITWKLYEPLRAYSDSANFPDDQF
                     GMNLDVVSFQLTAAKEFAWGERSAALGIGLQLQRADLIYTNVFFRRNPITNSPDEPLY
                     DVMQDYPWDVITQWSRSDGEGWGFGVNLGAMVQLSERTQAGVTFNLPFPITISGTS"
BASE COUNT         1705 a         3194 c         3394 g         1450 t
ORIGIN      
        1 ccaccgcagc agcgagagaa agcgctcctc ggaggcctcc acccggaggt tggcggccca
       61 gtacgtcact cccgtcatca tcgccagcac ccccagctcc actccgaccc ggcgggtcag
      121 cggcggtccg acgcgttcca accggtggaa gagccagagg acggccggca ggccggcgag
      181 cgagagcagg tcccagtccc ggcccattcc gatgaccggg ttcaggcaca gggcgaagca
      241 cagtccgccc agcgaggcgg cggcaagaaa gaccgcgatc ggctcggcgg cgagtccagc
      301 gggacgcagc cgcgccgccg ccagaaccac cgcccccggg aacaacacca gcgccagatt
      361 gaacagatcg cccagatgtt tgagggagaa gaccgcatac tccggcgcct cgatgcggcc
      421 cgaccacggt gggagaacga tccagttgcg gtcggtcggg ccgaagagga accagagata
      481 ggcggccagc gccccgagcc cgagcgttgc gagaagcagc cgcgaccgcc gccgagaaag
      541 ttctccggtg cgcgcggcca cgactccggg cacgagggcc gccgcggcgc ccgccagcca
      601 gaccgcctgc acgtggatac aaagcgccgc cgcgtaaacc agcagcgctc ccgcgagcgc
      661 ccccgcccgg tgctcctgca accacaccag cgccaaccgg aaaaaccaca ccgccgccgc
      721 ccagagcagg ggatagaact ccacgtagcc gaaatacagc agccccgccc cggagaagag
      781 cagggtggca agggccgcga cccgatgcac cggatgtttt accaggagtg cgaccagggg
      841 gaacaggccg agcagggccg cgataccggc ggcgatcgag atcgcccgga acgccgcctc
      901 cgccgtcccg cgcgtgtacc cgccgagcaa cccctggagc gtgcgcacga gcgggatgga
      961 accgaactcg gcccacttgc ggacatgccc ggctgcctgc gagaaattgg ccgtccaggt
     1021 gagaccgtcg ccgagaaaat gcgtctcggc ctggaagaac cagaaggcgg cgccggcgag
     1081 cgcggccagc cctaatcgcc attgccaggc gagccggccg aagaacaact cgtgccccca
     1141 ggcggcgatc agggccggga ccggcgcgta ccggggatgc agacagagca ggtggaagat
     1201 caggccgagc caggcgagag cttcaaaagc cggcggcagg taccggagat ggttcagtcc
     1261 ccacaggccg aggccgccgg cgcggccggc ggcaatgaac gcgagggcga atataatgat
     1321 caggacggcg gtagcggtat ccgtaaacgc aaaacgcgac tgacagcggt gcggccgggt
     1381 gctcacggcg gttcactccc caaaagatga aaggctgttg ttcggagaaa gtataggcgg
     1441 cgtttaatcg atgtcaagag cctgggaggc aaatgtgatc agcttgatgg ccgcaaagga
     1501 gatcgtccgg cggtcgccgc gcacgtggcc gaaatcggcc acccggtccg ccccgaaaga
     1561 agcatcgacg aagatgtgcc cgaaaagcag tccccgcagc tgcagcgccg ctgtgaatgt
     1621 cttctccacc acgccggttg ggaacggctc gcggtaatcc ccttcaacct caagccacgg
     1681 ggtgtcccag gcggcgttga cgcggccttc cccctggcgg cggtaggcca gccgcacgcc
     1741 ggcagccaga tcctccccgc accagcgccg gatgagcaga tcgagaagat cgtagtcatt
     1801 ccccagcgcc gccccgagca gttcgctctc gaacgtgtac cggttgcggg ggtggctctg
     1861 gttgaaggtg cggttggtga cgcgcgtgta ctccgcccgc aggtcgagtc ggggcaggaa
     1921 ctcggcgaca tacgcgcccg ccaacaggcc gatctcgttc ggttcctggt cgctctgcga
     1981 ggcccggtcg acctggtagt catcgaccag cagttgcccg tagagctgca ctccgcggcc
     2041 gggttcgtag gcgaagtcga caccgaggaa ggtgttgtcg tccacgccgt cgttgagctg
     2101 cgaggcgtga tagaacagaa tcgggttgag gtagtacaat tccacctgcc gtccggcgcc
     2161 cccgaacacc gccgtttcga agaatccgat ccgcaggcgg cggctcaggt ggatgtcgag
     2221 ccgatgcccc gcgaaatagc ggttctcgac cggcacagcg ctgtcggcgt ccggatcaag
     2281 gctgtcgagc cgggcgagcc ggtaggagag caccagccgt ccccagcggt aggcgtaccc
     2341 gaaaccatcg agcgccgcgt gcggggccag cacgagcgac cggcgcggcc cccagaacga
     2401 ggcgtaccgc ccgagcgcgg cggtgaaggc cccggcgctc aggtgaacga aggcctcgtc
     2461 gatgtcgccc gccagaccgc gccacttctt gccggtgtac gccggatcat ccgcaagcgc
     2521 ctcgtcgagg cggaagctgc cgtagacaaa aatcctgtcg tgcgggcgcg cggccagagc
     2581 gccggagaac gactcatagc cgactgtcgg gtgacccttc gcgctccgga aatcctcacc
     2641 gaggcggcct tccacgcgca ggtccgagcg gtcgagccgg tccagccagg cgaacggacc
     2701 gagcgcctcg cgggcaaggg cgagcgggta tggaccaagc tggcggtcgg ccgtgtcgag
     2761 cgtgagggca tcggcgcgtt cgaggcggtc atagagaaat tcgaactccg ggctgcccag
     2821 cgggagcacg ccggcggcct tgatcgccca gggcagcagc agcaacaccg ccagcagcgc
     2881 gagtatgcgg aaaagcgcct tcatcgtcaa accgcgccgt cgcctttgag gaccgtgggg
     2941 acggtgcggg cgaggatctt ggcgtccagc cagagcgacc agttgtcgat gtactggaga
     3001 tccagcttca tccagtcctc gaaatcgatt ttgttgcggc cgctcacctg ccagatgcag
     3061 gtgaggccgg gtttgaccga gagcttgcgg cgctgccagg gttcaaagcg ggccacctcg
     3121 gaggggagcg gcggccgcgg gccgaccagc gacatgtcgc cgcgcagcac gttgtagaac
     3181 tgcgggatct cgtcgatcga gaaccggcgc aggaaccgcc ccacccgcgt caggcgcgga
     3241 tcgttgcgga ttttgaagac cggcccggac atctcgttca gcccccgtag cgactccttg
     3301 tgcttctcgg catcggctcg catcgtgcgg aacttgtaga gcgaaaaggg ccggccgttg
     3361 gcgccggcgc gccgctgcgc gaaaaacacc ggcccggggg actccagctt gatggcgagc
     3421 gcggtcacca gcatgagcgg cagcgtgagg atgaggccga ccagaccgcc caggcggtcg
     3481 agcgcgttct tgacgaacag ggcggccgga ctctccggca cgcgccgcca gaccaccgcc
     3541 ggcgaaccgt tgagcgtcgc cggcaccatg cgcgcgatcg tcgtcgggta cacatcgggc
     3601 agcagcgcca ccgccacccc gagctgttcg gccgccgtca gcagcggcgc cgtacgggag
     3661 aggtcctccg gttccacggc gagaatgacg gcgtcgacct ggacccgcgc ggccagcggc
     3721 gtgagcaggt ccgggtggcc cagcagggga atgtcgcggt agcgccagaa gccggtgcgg
     3781 cggtagtcga ggaatccgtc gatcgccgca tcaagctcgg gggcgttgag aatcatgtcg
     3841 gccagcgcct gggcgcgcgc cccggtgccg accacgagga ggcggtttcg ggcgcggcgg
     3901 aggcggtcgg gcagcggcga gcgggccagc cgccggagaa tcagccgcgc cccgaccaac
     3961 agaccaagct gggtcacgaa attcagtccc agaaagaccg gcaccgccag gcggggcagc
     4021 ggccagtcca gcaggtaggc cgccgccagc acgagcacgg caaagcgcgc ctcggcggcg
     4081 agcgccgcgc cggcgtattt ccgcaccggc ggagcgtcga gcaccggacg cgggtgcggg
     4141 tggacgaacg cgagcagccg cgctagggtg atcgcggcgg cgatttcgac cagcagcccc
     4201 agcgacgacc cggccgccat gagtgtcgcg cagagcgcgg cgacagcccc ggcatgggac
     4261 cccggagacc cggccaggcg gaggaggaac tgacggcgac ggtgggtgag gtgcatacga
     4321 tcaggctctc caatgatgat cttcccgcgc accgcgcgtc ggtcgcctct cgtctcggcc
     4381 gtcctcccgg cgggtccgca gggagtcgcg cgaggatccc gaccgaagcg acgggcgctt
     4441 cggtcggtgt tccggaggtg cagctatgaa cagccgaagt tttttcatac ggcgactgag
     4501 cggttcactc ggtgtcgcgc agggcggcca tgaggaggtt gccgctgcgg cccgcgccct
     4561 tgcgggccgc ctgggcgctc aggcgcatcg tcatcacccg cccgtcgatc gtcttcatgc
     4621 ggaacgactc cgggggcatc gcttggaagg cctgcagcag gcggtcgttg gacaacggcg
     4681 actgcacccg gtagcgcagg tagtgctgca tcgccgggta gccgtcgaga ttgtcgatcg
     4741 cgatccgcgc gcggctcagg tgattaccga cccgcgccgg gtcggcgacg cggaggagga
     4801 tgaaatcggt ggcgctgagg cggcactgca cgccgaggcg ggtgagtccc tcggccaggc
     4861 ggagcgattc ctgatgcccc tcgcgcaggc gcgcggccag cgcttctcct ccgggccacc
     4921 ggtcgatcag cagacgggcc tcggcggccg gcagcacgct ctcggggaga gcggcgcgaa
     4981 ggcgggcgat cgtgcggggg caggcgaggg cgtatccggg ctcgtcgccg ccgagtgcgc
     5041 ccccgggcgc aaacgagcgg agaatgatca cattgggaca gtcgctcagc agcgggagcg
     5101 ccgtgatgcc gaagtaatcg tggtagtgct catcgaccag cacggccccc tccgggaccg
     5161 tctccgccat ccgctgcaca tcggccagcg agtagttcgc gccggtgacg cggttcgggt
     5221 tggccgcgca gaggatgtcg tgggggagca ccgtcccctg cagagcggtc tgcaggctgc
     5281 cggagaaggg cgatgggccg atgtgttcga tcacctcgac ctcggcccgg tcggcggcca
     5341 gctcgacctc cggggtcaca tggccgatcg cctggaggcg gcagcccggc cgatagagcg
     5401 cccccagcaa gccggcgaga aacgggccgg ccgccgcgaa cggatgcacc atcgagggac
     5461 tggtcatggt tctctgggcg atcgcctcga gcaggtcgga gcgatcggtg gtgggcgtgt
     5521 cctggagagt gttcatacgc ttacctcata ttccggcggc gctgccgccc gcgtctgtgt
     5581 cggggagaga gggcgaccgc cgcggtggga ccggccgact gccagtcgcc gccgggtcgc
     5641 gggggtcgct gtgagtgagt ggttgccatc ggcacaacat tccttgtcgc tgccagattt
     5701 cctttgcggg caggaattgc aagccctgtg ccgaatggcg cgggaaccgc cagcggaggc
     5761 ggaactcgtt gtggcgacgg cggtaaggcg tgcgtgtgag agtcccgggc ggcggcgtgc
     5821 gaccaccccc ggcgggggcg cgaggcagga atttccctcg gctagcgaaa acaattccgc
     5881 ccgacaaaaa aacgccgccg gtgctgtcgt cacagcgccg gcggctacag gaaggtaaag
     5941 gaaataaatg caggaatctt ggaccgctag agtttgctca ccaggtaggc cgacgtcacc
     6001 agtccggccg tggctgtgac caccgtggtg accgtcttga gccagtcgcg gtcgtgcttg
     6061 atctggctgg gtacgacgat gatgtcgccg agccggacag ctttgcccat gacgtcgccg
     6121 ccggcgaaca cttcgccgcc cgcgcgaatg agccgcgtct ctttcttgtc ggcgcgccgg
     6181 gtgaaattgc ccgcccgctc gatgtaatct ttcactctgc ggccctccac gtacttcagc
     6241 gtgccgttgg cccccaccgc gccgaggacg gagatgccgg agggagtcgg cgggatgaac
     6301 acccggtcgc ccggttccat caccacgtcc ccttccgaac cgcccgtggc caggatgcgc
     6361 tccatgtcga tgatgatccg gtccatcgac tcatcgcggt agctgaaaaa gctgtcggtg
     6421 gtggggacgc cgttggagtc gagcacgatg ggctgcgact gctccaggag cgcggggaca
     6481 ttggcgcgct ggaggttgtc gcggatcgag cgccgctgga agatgatgcc ggtcgggaaa
     6541 gcgttggcgg tgaacccgcc ggcccggccg agcagatggt gcagcccctc gtgccgcccc
     6601 tccaggaagt acgtccccgg atacatcact tcgccgctga cgacgacgct ccggttggcg
     6661 tcccactcgg gaatgcggcg gatgaagagg tggtcgtctt cgcacagcag ccggcggtag
     6721 ccgccggagt cgctcaggct caccggcaga atagccacgt tgccgagcga gtcgaggcgg
     6781 gcgagttcgg cctcgccggc gtcggcgccg cgcaggaacg agccggccag gaagatcagg
     6841 tcgccgaccg tcatcccgtc gtagagcggg tacttgcccg gtttatgcac ctcaccttca
     6901 atgtacacga actgctcgcg gcgcacgtcg tcgatgccgt acacgtggag cgaatcgcgg
     6961 tcggccagca cgatgtcgga ggccgggtcg ccggccagcg cccgggtgag atcgatcgga
     7021 atcacctcga cccgccggtc ggggtagcgg cgaaacacat cggcccgctc gaaatagacg
     7081 tcgagcggct gcagctggcc gcgggcgatc aggtccgaga cccgcgtgga gtcggtgcgt
     7141 tcgtagcagc cggggtgttt gacccggccg ccgaccgcca ccatgttggt gcgcaggtcg
     7201 tagatggagt acacggtgac ccggtcgccg tcggccagcg cgatcgtgtc gcacggctgg
     7261 gccggcccgc agttgagatt gaggtcgcgt acgagccact cgccggccgg cgagatccgc
     7321 tcgagcatga cgcggtcgag gtacgccgcc gccgtcggcc ccccggccag ccgcagcagg
     7381 tcggatacgt tctccccgcc cgacaactca tagatcgccg gccgccggat ctccccccgc
     7441 accgccaccc gcggcccggt caccgggaca aacacgacat cgcccgtctg cagccggatg
     7501 tccgaggcgt tgtgcccctt gaggagcaga tcgtaaaggt cgatggcggc ggccgccgcg
     7561 cccccgcgcg tgaccgcaat ggcgcgcatc gtgccgcgct cgttcggccc gccggccgtg
     7621 aacaccgcgt tgagcaccga cgtgagcgac gacaccgtgt aggcgccggg acggcggact
     7681 tccccggtca cgtagatgcg gatcgagcgg atcttcccga gcgaggcggt gagttcgaaa
     7741 tcggagtaga tcgccgcgag gcgctctttc atccgctggc gcatttgctc cagggtgagc
     7801 ccccaggcga taaggtcgcc gactttcgga atgaacactt tcccttcccg gtcgagcgtg
     7861 aggttgtact cgtgttcgac ccggccccac aggtggacga tcacgttgtc gcccggtccc
     7921 agcacgtagt cgtcgctcga cacgaggtcg gccggcgggt tggtctcgcc ggcgttctca
     7981 aagagttcca tgccgaaggg gcgcaggtcc tcgaacggtt cgagcgtccg gtcgcgctca
     8041 aaaccggtgc gcaccggggc cggcggcgac ggcgccgcga cgggcgccgc cggcgggccc
     8101 ggcgacgccg tgtcctcctc aaagatccgg gggctctcgt agtagcccgg ctcgggctgg
     8161 ggggccggcg ctgacttctg ctcgtactgg cggcgcaggc gcgcgatgtc ctcggtgctg
     8221 agcgagccaa gatcctgcgc cattgcccgg cggaaccagt tgatcgagac gagcacgatc
     8281 accaggagaa tgaagatgat gagcttgcgc atggacgata ccttcctgta atgcaacttc
     8341 cttttgcctg acgaccgcct ccggcggccg ttgccgcagg caaaagcacc ccgtgtgccg
     8401 gagatcgggg cgatgcggca agcgcttgtg cgcgttgcgc ctcaggctgg caggcggccg
     8461 ccgcgacccg ccgcagcagg ttatgcgtgg gcaaagttgt cccctgtggc gggaagtttt
     8521 ttccggagcc ggagacgaca atctgggggc ggggaggggg agtgtgcggc ggggctttca
     8581 taagagcaaa gcgggcaacg tctttcaact tgtccaattt tctccggcgc gccgtatgga
     8641 ctgcacgccg gcgccccgtc gcacgtctgt gtgtcgaatg ctaacgtgcg tgatggcata
     8701 tgcttaatca ggcgcgcaga cttacattat caggtggaag tccttgacaa gtccctgcgg
     8761 ccgccttaat atggttgcct gtttgttgac cgaagccgtg gaggagatac tgtcggcccc
     8821 tggtggtttc tctccgactc gttcctgtcg gcccccatcc gtgcttcgat cactgaccac
     8881 gagagagacg catttgagat gatatcaaac acggagatca ggatgggaat tgcgaaattc
     8941 tctgccacgg tagcgaccgt tctggttttg ttgtcctccg ggatcagcgc cagcggcttc
     9001 gaaaactcgg gcgtcgggat gaaagcccgg ggcatggggg gcgcgttccg ggcgattgcg
     9061 gatgactgga ccgccgccta ctacaacccg gccggctatg cctacatcct ggacaaccag
     9121 ctgggatcgg cctgggcctt tgtgcaccac cgcgacgagt tcgtgccgaa cacccgctgg
     9181 ggcggcacct tcgaccgcgg tgtgttcgac ggtcgcacca actacaacaa ccatgagatc
     9241 ctcaccaatc cctccgccgg catcctcctc cgcctcccgg tggccggcga aaccgtgttc
     9301 ggcctgagcg cctaccagcg gttcgaccgg aacatcacgt ggaaactcta cgagcccctg
     9361 cgcgcctaca gcgacagcgc gaattttccc gacgatcagt tcggcatgaa ccttgacgtc
     9421 gtctcattcc agctgacggc ggcgaaggag tttgcctggg gcgagcgcag cgccgccctc
     9481 gggatcggtc tgcagttgca gcgcgccgac ctcatttaca ccaacgtctt tttccgccgg
     9541 aacccgatca cgaattcgcc cgatgaaccc ctctacgacg tgatgcagga ttatccctgg
     9601 gacgtgatca cccagtggag ccgcagcgac ggcgaggggt ggggattcgg ggtcaacctc
     9661 ggggcgatgg tgcagttgag cgagcgcacg caggcggggg tgacgttcaa cctgccgttc
     9721 ccgatcacga tcagcggcac ctc
//
LOCUS       DAOPOE010000104         9711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7933_length_9711_cov_4.690969, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000104 DAOPOE010000000
VERSION     DAOPOE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_9711_cov_4.690969"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..773)
                     /locus_tag="PKM94_11615"
     CDS             complement(69..773)
                     /locus_tag="PKM94_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67323.1"
                     /translation="MDEPFTLSLLRCLYGAAPDPCEDSTFDPLTFDQLAPYRHPGFPD
                     DSIFWFRIHDYNASSMGVTTPIRKCYPEMPDPRTLPPDSLTPEMYTDDGYLKFFEYEF
                     EIEGLLPSVPYWLNVTAFDFGSPAMGVEPLESPRDSGLVKAFPLHEWEAPISGSDRIY
                     VFPNPYRTDAGYRDQGFEGRNQNDRWDERVRAIWFANLPPICTIHIFTLDGDRVRTLD
                     HDMSRPIRLTAGRSGT"
     gene            complement(686..2599)
                     /locus_tag="PKM94_11620"
     CDS             complement(686..2599)
                     /locus_tag="PKM94_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67324.1"
                     /translation="MANSRKLGTKIAVMSATLLLPLSSHALQEPSQFIRSVAAAVAQG
                     DSLPDHYSRTTTHIVNQLALGVSNRGAIAQSGRDAFTGDTILGHCEFPRGSRRDYLEQ
                     IDLWLGVQGSRDPVVSNGYYYLAEEAIAGEWLPSSPPGEFVRRSITDRFGGPDAEAVS
                     EEDLLCTYDDSLGLWKLGDHLTPNPLSLGAFVRQASYAWSMTYAEDFAIVRLDIRNEG
                     TMLWRETYAGLHIIPSVGLRDPCEDCGPKWTVCGIRTVAPAAFGCDHLDTLNTAWHAT
                     ASGDPYEGEWLENPLYDSFRKCYYGSTRGIIGILPLCAPHPAYTLHYNWWASVLHPDP
                     EEGIFSFQPSQDALVPSYVPGFVYVNTDKSRYRLLAANEVDYPSYQASRIASTPFSGW
                     LPVPPELANAVSSGVWPEALLSWGPFDVSPGGRFSIVFAVVGGEDFHTDPSNYRHLPA
                     RPDLYEAGVNFSDLDRNALMAQWVYDNPGIDTDGDGYSGEYRVCVRDSMLIGDRWVVN
                     KADTQWYRGDGIPDYRGALSPPAPHLWVTPIHNGFHVRFNGEKSETAKDIFLQRVDFE
                     GYRIYLGRDERRTSLSVVASYDREDYTSTCFWPIRKEVSPGGSWMNRSPCRSCAVSTV
                     LLPIPARILPSTR"
     gene            complement(2771..4891)
                     /locus_tag="PKM94_11625"
     CDS             complement(2771..4891)
                     /locus_tag="PKM94_11625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD67325.1"
                     /translation="MAALAVAVRWVYLLELASHPGFTVPMVDEKWHWEWAREIIHTSF
                     FGQGTWFRAPLYPYVLAFLAFVTGESVFWAKFLQLLITGGTAFFLVGLGERLFSRPVG
                     LLAGVVYAVYGTALYYESMFLISVVFLFFLVWGIYRLVAFRASERWRSWLLTGAVFGL
                     AAISRPNVLLVIPLFLLWVFYVSPRRQRFLTRLRRPIILLAGLLIVILPVTVRNLLVT
                     GDFILISSQGGVNLYLGNNPLANGLSMQMGEVELTEGLTWDRFAPAAHEAAERLAGRR
                     LSESESSDFWTRRSLQFIKDNPAAFAGLVWRKTVYLVSGFENSDNSDIYYERGKSFLY
                     SLLLWDGPIDFPFGLLLPLTLAGLYLRRRETDRLAPLYLFLIGYIPTIVLFLVTARHR
                     LPLVPFMIILAAAGLVKAVELTRRRRLKELAVAGVIFLVPLVLCNRLWYDEGGRNEFQ
                     VHFNEGIKYEHLGDYAAAEREYLAADRSFGGSAALLTNLGRVQHQLGKLDEADRAYRR
                     ALGLDPTFYRALNNLGLLVGERGNLDSSLVLFVAARQHYDSMLARTNELGLIYMNIGK
                     GLEQFGRPDVAVAYFDSAVHAAPQSPEVSYQAAAFFARQERYELSDSLFLRGQALTEP
                     SAADFFNWGLSFMERRRFADGIYMLDKALRRDSTLYQAHYLIAVAYREGGMPPDSVNA
                     HLNAALRSNPNYEPALRFREQIGR"
     gene            5549..6373
                     /locus_tag="PKM94_11630"
     CDS             5549..6373
                     /locus_tag="PKM94_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD67326.1"
                     /translation="MSKRVNMLMVAAAVLVMPALVLAGSAHQFAVGKSVASQTEANVV
                     TVPLVVTNEANLAAMDIPLKFSEGVTLQAVEFDGTRTEYFDLKIANINNENRTVIIGL
                     LPQITPDFKPDLEAGTGVVANLVFRIDDPTAQNVSLETFTTENPGHELTFVYHTDDAG
                     QLGQYVVNPEFSTVSFSLTEAAGANLPKQFALNQNYPNPFNPSTTISFALPKPSHVEL
                     SIFNVLGQQVVKLADGQFDAGEHAVTFDASRMASGVYFYRIQTDENTETRKMMLLK"
     gene            7061..7930
                     /locus_tag="PKM94_11635"
     CDS             7061..7930
                     /locus_tag="PKM94_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67327.1"
                     /translation="MPTRLLMLGLILAGVAFFSCSDDDEATGWNDEFSFELTAGTVEG
                     DPVPNLRVSIWSLFDGLPIYRRAAGETMEPQVMTVIGFDLPQACSVSLAVYDLAGGLE
                     TTLVAQPLDAGVYSVAWNAGAGSEVLPDGVYRGVFRAASGSGEPLFQDTICMVLWRPD
                     PEANVYGYTDAGGRFTTRDILRFPHLFPLPPLRHTNEVGTELGTFSYRSWVMVSLKDT
                     ATGVHLEYLTEILNERNQIDYTWTPAQPDRAADLPQERGATGPCQTEGPATVLGPPPT
                     EFRLCQNYPNPFN"
     gene            8070..>9711
                     /locus_tag="PKM94_11640"
     CDS             8070..>9711
                     /locus_tag="PKM94_11640"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD67328.1"
                     /translation="MAKSDKSKQAAPAADGAVPGTAPGGLAVAPPEALDGCGFDRTNA
                     FAAAGIVLVAFAMYARTVAPTLSFWDCGEFIAVSSILGIPHPPGSPLYVLLGRLFSIL
                     PTAADPAMRVNLLSGVSSAFAAMFAYLIVVRLLRAWFSGPAVIDRLTVYLGGAAGALF
                     AAFGLTNWNNAVEAEVYGLTMALMLCVFWLGLRYRQHQETPAGDRIMFLAVFLSVLGV
                     GVHLMAFVALPIVALFFILKKESPAWVWFTVAAFLLLEMYLIFALSSRPGEVPYWVPV
                     TIVGAFYYLYMFSFERIPRVHVWFAVGFLAAMIPLAGEVIARIAGSEGAAGRVLTGAG
                     MAAFAALAAGAAYIGWSYSNKRGRMKDREAARAYLAPAGFVAAAVVMVALLFMFKGYK
                     PFLLATVILGALLLVLLRRYLRWEFLIAIVAIGTIIVEVKPFLLAVGLAAVVLALLGL
                     ARMLPGWKSALLMLAVALVGFSINAYMPVRAAREPAINENNPQTIAQTIDFLDRKQYQ
                     RISMVERMFVRRGSWENQFGNYQRMGFWRFFHEQYGLRG"
BASE COUNT         1889 a         2810 c         3272 g         1740 t
ORIGIN      
        1 gggttcaccg gggatctcga ccacccaata gtacaggccg gtctcgacct tctgatagtt
       61 ccggttgatc aagtcccact tctgccggcg gtgagccgga tcggccggct catgtcgtga
      121 tcgagggtgc ggacgcgatc gccgtcgagc gtgaaaatgt gaatcgtgca tatgggcggc
      181 aggttggcga accagatcgc ccggacgcgc tcatcccagc ggtcgttctg attgcggccc
      241 tcgaacccct ggtcgcgata cccggcatcg gtgcggtagg ggttggggaa gacatagatg
      301 cgatcagatc cggagatcgg ggcctcccat tcatgcaggg ggaatgcttt gaccaatccc
      361 gagtcgcggg gggactcgag cggctcaacg cccatggcgg gcgagccgaa atcaaacgcg
      421 gtgacgttca gccagtaggg gacactgggc aggagcccct caatctcgaa ctcgtactcg
      481 aagaatttca aatagccgtc atcggtgtac atctcagggg tgagagaatc aggcggaaga
      541 gtgcggggat cgggcatctc cgggtaacac ttgcgaatgg gcgtagtcac tcccatcgag
      601 gaggcgttgt agtcgtggat gcgaaaccag aagatcgagt cgtcggggaa gccggggtgc
      661 cggtacgggg cgagctggtc aaaggttaac gggtcgaagg tagaatcctc gcagggatcg
      721 ggagcagcac cgtagagaca gcgcaggagc gacaaggtga acggttcatc catgagccgc
      781 caggtgacac ttccttccgg atcggccaga agcatgtact tgtgtagtct tcacggtcat
      841 acgaagcgac gaccgagagg cttgtgcgtc gctcgtcacg gccgaggtag atgcggtacc
      901 cctcgaaatc gacgcgctgg aggaagatgt cttttgcggt ttcactcttc tcgccgttga
      961 agcgtacatg gaatccgttg tggattggag taacccagag gtgcggggcg ggcggcgaaa
     1021 gggccccccg gtaatcgggg atgccgtccc ccctatacca ctgggtatcg gctttgttga
     1081 ctacccaccg gtcgccaatg agcatggagt cacgcacgca cacccggtac tcgccggagt
     1141 agccgtcacc gtcggtgtca atcccgggat tgtcgtaaac ccactgcgcc atgagggcgt
     1201 tgcggtcaag gtcggagaaa ttgaccccgg cttcatagag gtcgggacgc gcgggcaggt
     1261 ggcgatagtt actgggatcg gtgtggaagt cttcaccgcc gacgacggcg aagacgatcg
     1321 agaaccggcc gccgggtgac acatcgaagg ggccccagga gaggagggct tccggccaca
     1381 cgccgctgct gaccgcgttg gccagctctg gagggaccgg gagccagccg ctgaaggggg
     1441 tcgacgcgat ccggctcgcc tggtaagacg ggtaatcaac ctcgttagcg gccaggaggc
     1501 ggtaacggct cttgtcggtg ttcacgtaca caaagcccgg gacgtacgag ggtacgagcg
     1561 cgtcttggct cggctggaag gagaagatcc cctcctccgg atcaggatgg aggaccgatg
     1621 cccaccagtt gtagtggagg gtgtaagccg gatgtggagc gcacagaggg aggatcccga
     1681 tgatccctct ggtcgagccg tagtagcact tcctgaaaga atcataaagc gggttttcca
     1741 gccactcgcc ctcgtacgga tcgcccgagg cggtggcatg ccaggcggta ttgagagtat
     1801 caaggtgatc gcagccgaaa gccgccggcg ccacagtgcg aatgccgcag actgtccatt
     1861 tgggtccaca atcctcgcac gggtcgcgaa gtccaactga gggtatgatg tgaaggccgg
     1921 cgtaggtctc acgccatagc atagtgccct cattgcgaat gtctaatcga acgatagcga
     1981 agtcctcggc ataagtcatc gaccatgcat acgatgcctg cctgacgaaa gcgccgaggc
     2041 tgagcggatt cggggtgagg tggtccccga gtttccacag gccgaggctg tcatcatacg
     2101 tgcagaggag gtcttcctca gagacagctt ccgcatcggg tcctccgaaa cggtcggtga
     2161 tcgaccgacg gacaaattcg ccaggcggcg aagaggggag ccattctccg gcaatcgctt
     2221 cctcagcaag gtagtagtag ccgtttgaga caaccgggtc gcggctgcct tggacaccga
     2281 gccatagatc gatctgctcc aggtagtcgc gcctgctccc gcgcgggaac tcgcaatgac
     2341 cgaggatggt atcgccggtg aaggcgtcgc gccccgactg tgcgatggcg ccgcgattgg
     2401 agacgccgag agcgagctgg ttgacgatgt gggtggtcgt acgggagtaa tggtcgggca
     2461 gagagtctcc ctgcgcaacc gccgctgcca cggagcgaat gaactgtgac ggctcctgca
     2521 gcgcatggga agagaggggg agaagcaggg tcgcggacat cactgcgatc ttggtcccca
     2581 gtttccgcga attggccatt aggccccggt ggttcccgct catctccgac cccactgctt
     2641 atgcaaatcg gcagagtact ctcttgaagg ataaagtgtg cgcggcgaca cgtcaagcgc
     2701 agacgacagg gcgccgacag tcttcggatg tcgcgttccc cggaccccga cctcccgcct
     2761 tgccgggcgc ttagcgcccg atctgctcgc ggaagcggag agccggttcg tagttcgggt
     2821 tggaccggag agcggcgttc aggtgggcgt tcacggaatc cgggggcatg ccgccctcgc
     2881 ggtaggcgac cgcgatcagg taatgggcct gatagagcgt ggagtcgcgc cggagcgctt
     2941 tgtcgagcat gtagatgccg tcggcgaacc gcctccgctc catgaacgag agcccccagt
     3001 tgaaaaagtc ggcggcggag ggttcggtca gcgcctggcc gcgcaggaaa agcgagtccg
     3061 acaactcgta gcgctcctgg cgggcaaaaa acgcagccgc ctggtaggag acctccggcg
     3121 actgaggagc agcgtgaacg gcggaatcga aataggcgac cgccacgtcg gggcggccga
     3181 actgctcgag ccctttgccg atattcatgt agatcagccc gagttcgttg gtacgggcga
     3241 gcatgctgtc gtaatgctgc cgggccgcga caaacaggac gagcgacgaa tccagattac
     3301 cgcgctcccc caccagcagg ccgagattgt tcagggcgcg gtaaaacgtg ggatcgagac
     3361 ccagggcgcg ccggtaagcc cggtcggcct cgtcgagctt gcccaactgg tgctgtacgc
     3421 ggccgaggtt ggtcaggaga gccgccgatc cgccgaacga ccggtcggca gccaggtatt
     3481 cccgctcggc ggccgcgtag tcgcccaggt gctcgtactt gattccctcg ttgaaatgca
     3541 cctggaattc gttgcggccc ccctcgtcgt accacaggcg gttgcataac acgagcggga
     3601 ccaggaagat gacgccggcg accgccagct ccttcaggcg ccgccggcgg gtgagctcga
     3661 cggctttcac cagaccggcc gccgcgagaa tgatcatgaa cggaaccagc ggcaggcggt
     3721 ggcgggcggt cacgagaaag agcacgatcg tcgggatgta gccgatgagg aagaggtaca
     3781 gcggggccag cctgtcggtc tcccggcggc gcaggtagag gccggcgaga gtgagcggca
     3841 acaggaggcc gaaggggaaa tcgatcggac cgtcccacag caagagcgaa tagaggaagg
     3901 acttgccgcg ctcgtagtag atgtcggagt tgtcggaatt ctcaaaaccg ctcacgaggt
     3961 acaccgtctt gcgccagacc agcccggcga acgccgcggg gttgtccttg atgaattgca
     4021 gcgagcggcg cgtccagaag tcggaggatt cggattcgga caggcggcgg ccggccagcc
     4081 gctcggcggc ctcgtgggca gcgggcgcga agcggtccca ggtgagcccc tcggtcagct
     4141 ccacctcccc catctgcatc gacagcccgt tggccagcgg gttgttgccc aggtagaggt
     4201 tcacgccccc ctgcgaggag atgaggatga agtcgccggt gacaaggagg ttgcggacgg
     4261 tcaccgggag gatgacgata agcaggccgg ccaggaggat gatcgggcgg cgaaggcggg
     4321 tcaggaatct ctggcggcgg ggggaaacgt agaacaccca gagcaggaac agcgggatga
     4381 cgagaagcac gttggggcgg ctgatcgccg ccagcccgaa aaccgccccc gtcaaaagcc
     4441 acgaccgcca ccgctccgag gcccggaagg cgacgaggcg gtatattccc cagacaagga
     4501 agaacaggaa gaccaccgag atgaggaaca tcgactcgta ataaagggcc gtgccgtaca
     4561 ccgcgtacac cacgcctgcc agcaggccca ccggtcggct gaaaagccgt tcgccaaggc
     4621 cgaccaggaa gaaggcggtg ccgccggtaa tcagcagctg gaggaacttc gcccagaaaa
     4681 ccgactcgcc cgtcacgaac gccaggaaag caaggacata gggatagagc ggcgcccgaa
     4741 accaggtccc ctggccgaaa aacgacgtgt gaatgatctc ccgcgcccac tcccagtgcc
     4801 acttctcgtc aaccatgggg acggtgaacc cgggatggga ggccagctcg aggaggtaga
     4861 cccaccgcac ggcgaccgcg agtgcggcca ccagaagagg gtacttatac gttcggagga
     4921 aagctctcat atgtgaggcg ggcgcgttgt tggtcaccgt ctccgctggg gcgggacgac
     4981 cccttgtgtc cattcagccg gtcaggttga agtcggcgat atttcaggcg acccaaattt
     5041 gcgccccgac ggggcgaagt caagccctaa tcgcagagtc tgcggcgggc ggggagaggg
     5101 accgggggga gggggcggcg gcgaaacggg gtcaacgtgg agccgcacgg cgcaggttgt
     5161 gcgccggatg cgatgcgaga ccgccgtgcg accggaaaag atttcccgaa atttcattga
     5221 aaattcatta taaaaaagcg gaactacgcc cgtgcgggcg ttgtactaat cgtaggacgg
     5281 ttgcaggccc actgggacgt tgtgcaggga atcggacgaa gcgagtgatt cgaaatcgga
     5341 aaagcgcctt gacagatcgc gaatcatttc gtatttattt aacagatccc cacgagaaca
     5401 ccgccgactg caaatcagtg acaagctgac ggacgattgc ttgctggcat ttcgtgttac
     5461 ggaagataca cgtggcaagg agcgcaacgg aggaatctgg aagctcggtc acggagaaag
     5521 acacaaccaa ctccaaggag gtagaacctt gagtaaacgc gtaaacatgc tgatggtcgc
     5581 ggccgcggtg ctggtgatgc cggcgctcgt cctggccggg tccgcccatc agttcgcagt
     5641 cgggaagagc gttgccagcc agaccgaagc caacgtggtg acggtgccgc tcgtggtaac
     5701 caacgaggcc aacctggcgg ccatggacat cccgctgaag ttttcagagg gcgtgacgct
     5761 ccaggcggtc gaattcgacg ggacccgcac cgagtatttc gacctgaaga ttgcgaacat
     5821 caacaatgag aaccgcaccg tcatcatcgg gctgctcccg cagatcaccc cggatttcaa
     5881 gccggatctg gaggcgggga ccggggtggt ggccaatctg gtgttccgga ttgacgaccc
     5941 gacggcgcag aacgtttccc tcgagacgtt cacgaccgag aacccggggc acgagctgac
     6001 gttcgtgtac cacaccgacg atgccggcca gctgggccag tatgtcgtga acccggaatt
     6061 ctcgacggtt tcgttctcgc tgaccgaggc ggccggcgcg aatctgccga agcagttcgc
     6121 gctgaaccag aactacccga acccgttcaa cccgtccaca accatcagct tcgccctgcc
     6181 caaaccgtcg cacgtcgaac tgtcgatttt caacgtgctg gggcagcagg tggtgaagct
     6241 ggcggacggc cagtttgacg cgggcgagca tgcggtgacg tttgacgcct cccgcatggc
     6301 cagcggcgtg tacttctacc gcattcagac ggacgagaac actgaaacgc ggaagatgat
     6361 gctgctgaag taagcggccg atacgtgaag caggcgttaa gcaggcgatt ctgaactgaa
     6421 agttttctgc ggaaactctc cctcgcgacg gcgggggaga gttctttttt gtggctgcga
     6481 ccgccggaaa cgacgcctca acggccgtga cgacggctcg agcctccggc agtcaaccga
     6541 tgcggggcac ggctgctcca gggcggctga tggcggtgcc aatctggtcg ccaggaaaaa
     6601 gacgcaagtt gagactgaca gcagtttctg cgacaccgcc gggacagaat ttgcgaaaaa
     6661 ggcagttctg cgggcgatcg gaccaatcgt gctttgacgt aagatctgta ccgacaaaga
     6721 gatagccgtt tcaggcgatg attggcacgg agtttgtata tatctgagac atcgggtagc
     6781 gtagtgcgaa cagacccgca acctgacgca ctacatggaa gtatgagcag tatttcttgg
     6841 cggttagact gcttgtgagc tttcccgatt ggtgaggaga gagaggcctc gagtaatcga
     6901 ggccctttct tttttgggca gcccacggct cgtcccggcg acggtcccgt caaatccgag
     6961 ggcgcacgga tcaccccttg acacctctcc cgaccgcccg ttatccttcc attactaatt
     7021 gagcagggac tgacaggatt cgcccaccga ggaggtcggt atgccgacac gtctgctgat
     7081 gcttggcctc atacttgcgg gagttgcctt cttctcctgc tcggacgatg acgaggccac
     7141 cggatggaac gatgagttct cgtttgagct gactgccggg acggtcgagg gggacccggt
     7201 gccgaatctg agagtgagca tatggtcgct ttttgacggt ctgccgattt atcggcgcgc
     7261 cgccggggaa acgatggagc cgcaggttat gaccgtaatc gggttcgatc tgccgcaggc
     7321 ctgctcggtg agtcttgcag tgtacgacct tgcgggcgga ctggagacca ctctcgtggc
     7381 gcagccgctc gacgccggtg tctactcggt cgcgtggaac gccggtgccg ggtcggaggt
     7441 gctgcccgac ggcgtgtatc ggggcgtgtt ccgcgcggcg agtgggtcag gggagccgct
     7501 gtttcaggat acgatctgca tggtgttgtg gcggccggat ccggaggcga acgtctacgg
     7561 gtataccgat gccggggggc gattcacaac ccgggatatc ctgagatttc cgcatctgtt
     7621 cccgctgccc ccgctgcggc acaccaacga ggtcggaacg gaattgggga ctttcagcta
     7681 ccgaagctgg gtcatggtct ccctcaagga tacggccacg ggggtgcacc ttgaatatct
     7741 caccgaaatc ctcaatgagc gaaaccagat cgactacacc tggacccccg cgcagccgga
     7801 cagggccgcg gatttgccac aagaaagagg cgcaaccggt ccctgtcaga cggagggacc
     7861 ggcgaccgta ctgggcccgc cgcctacgga gttccggctt tgtcagaact atcccaaccc
     7921 ctttaactga ccgggggggt ggcgggcggt cgcggagccc gtcgtttcct gcgtttcaag
     7981 ctttacacgg ttcggaaatc cgttatattc aagggttttt gacggacaag ggatgaatcc
     8041 gccggacacc agccggccaa aaatcgccta tggccaagtc tgacaagtcg aagcaggccg
     8101 ctcccgccgc ggacggcgcg gtgcccggga cggcgccggg cgggctcgcg gtcgcaccgc
     8161 cggaagcact ggacggatgc ggttttgacc gcaccaatgc cttcgccgcg gccgggattg
     8221 tgctggtcgc tttcgcgatg tacgcgcgca cggtcgcgcc gaccctctcg ttctgggact
     8281 gcggcgagtt catcgccgtg tcgtcgatcc tcggcatccc gcaccccccc ggctccccct
     8341 tgtacgtgct gctcggacgg ctgttcagca ttctcccgac ggccgccgat ccggccatgc
     8401 gagtgaacct gctgtcggga gtctcctcgg ccttcgccgc catgttcgcc tacctgatcg
     8461 tcgtgcgcct tctccgggcg tggttctccg gtccggccgt tatcgaccgg ctgacggtgt
     8521 acctgggcgg ggcggccgga gcgctctttg cggctttcgg gctgaccaac tggaacaacg
     8581 cggtcgaggc ggaagtgtac gggctgacga tggccctcat gctgtgcgtt ttctggctgg
     8641 ggctgcgcta ccggcagcac caggaaacgc cggcggggga tcggatcatg ttcctggcgg
     8701 tgtttctgtc ggtgctcggc gtgggggtgc acctcatggc gtttgtggcg ctccccattg
     8761 tcgcgctgtt cttcattttg aaaaaggaat cccccgcctg ggtatggttc acggtggccg
     8821 cattcctgct gctcgagatg tatttgatct tcgcgctgtc ctcgcggccg ggggaggtgc
     8881 cctactgggt gccggtgacg atcgtggggg cattctacta cctgtacatg ttctcgttcg
     8941 agcggatccc gcgcgtgcat gtctggttcg cggtcgggtt cttggcagcc atgatcccgc
     9001 tggcgggcga ggtgatcgcg cggatcgccg gatcggaagg ggcggccggc cgggtgctca
     9061 ccggagcggg gatggcggcg ttcgcggcgc tggcggccgg cgccgcgtac atcgggtggt
     9121 cctactcgaa caagcggggt cgaatgaagg acagggaggc ggcccgcgcc tatctcgcgc
     9181 cggcggggtt cgtggccgct gcggtcgtca tggtcgcgct gctgttcatg ttcaaaggct
     9241 acaagccctt cctgctggcc accgtcatcc tcggggcgct cctcctggtg ctgcttcgcc
     9301 ggtatcttcg ctgggagttc ctgatcgcga ttgttgcgat cgggacgatc attgtcgagg
     9361 tcaagccgtt cctcctggcg gtcgggctgg cggcggtcgt gctcgcgctt ttgggcctgg
     9421 cgcgcatgct cccggggtgg aagagcgcgt tgctgatgct ggcggtggcg ctggtcggct
     9481 tttcgatcaa cgcctacatg ccggtccggg ccgcccggga gccggcgatc aacgagaaca
     9541 acccgcagac gatcgcccag acgatcgact tcctcgaccg caagcagtac cagcggatct
     9601 cgatggtgga gcggatgttt gtccggcgcg ggtcgtggga aaatcagttc ggcaactacc
     9661 agcggatggg attctggcgg ttcttccacg agcagtacgg cctgcggggc c
//
LOCUS       DAOPOE010000105         9707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_7935_length_9707_cov_5.383133, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000105 DAOPOE010000000
VERSION     DAOPOE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7935_length_9707_cov_5.383133"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKM94_11645"
     CDS             <1..989
                     /locus_tag="PKM94_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67329.1"
                     /translation="PPAAVAATKTEPAPPAPSRVQTPSATPPTAEAPKAAAPPKTAET
                     PKPAAGAPTPKPALPSVAKPDTITADVPRKPDIKPEPPEPMRVSEANAPQPTPTAKPA
                     ATPAPTAGKPSTTPPPASVTAAPKPSPTAPPPAPKPLASTATVTPQAAPATPAPTPPT
                     AEESAEPEEDEDVPEPEDLEPAVESSDDESPSDEAAARGTARFRRSPTDPTKIKGTLV
                     AEFPTRLVIKYEPGGRRDPFQTLIDETKQYNSPIERRIPNVEGLRLVGVLQSPLGNSA
                     LFEDKDGYGYILREGDKIQKGYVLRIQTDRVFFQIFEYGWSRTVALNLESEN"
     gene            1030..2292
                     /locus_tag="PKM94_11650"
     CDS             1030..2292
                     /locus_tag="PKM94_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOD67330.1"
                     /translation="MRRLLPILLAVAVAVPAAAQNAGTPGEPIKNLQFQAAEIRSVVN
                     FLADYGGVNVVVAPEVEGTVTIRLVDVPWRTALDIIGRTYNLAVVDEKEGYIRVLPAE
                     DYRKEVSEQNRHLAETKELVPLQTKIVHISNSTSDDIVAAVKSLLTVRGQATSDPRSN
                     SIIVQEVPTNMDAVLAYISQLDQPAQQIKISTQLLEIFTEDLEELGVDWSVTGSTATD
                     GGHVNIGQTGEVLTARSADYAGRYTVHALGPNWNAEAVVKAIVNSGRGKIVAHPEITT
                     IDNKEARIQMGQKIPVKQFDESGNVVIVFEEVGTILTVTPHITAENQILMHLRPERST
                     YEFDPNGVVINTNNAETHVIVENGQTAVIGGLTTEDKVDAEVGVPLLKDIPLLGRLFK
                     FTQSRNEVRDLVIFVTPTIVENDLAMKK"
     gene            2434..4533
                     /locus_tag="PKM94_11655"
     CDS             2434..4533
                     /locus_tag="PKM94_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67331.1"
                     /translation="MAKRIFRCRAALAALAAAWAAAFLGGCSSNNDDGGGVALPTVTV
                     SASPSTVAVNASTVVEATVRNGGAPIADLVVTFAVQPAGAGYFTPPSAATDADGVAAT
                     IFTAAQAGTVALSASTEIGSAVRTASTGVVVTSSQQGTGSVAITVAPGLLLANGTDTS
                     LVTVTARQALGQPVPDGTPIVFAAGEKFADKDANGIWTPGIDSLVFDVNGNGNWDAMG
                     QIPSSIPVSGGTGQAQAVFVAGNSAGTVYVKATIGDETIGGSADKALQLSPNARISSI
                     FLHSDSINLAVRATGGMETAVLSATAYDGWGNPVPEGLPISFIITDGPGGGERLDTAG
                     YGPYSAVTNSQGTAQAPIQSGTAAGTVRIRAYCDTILSNAAQVMVSAGPPAHLVVGAD
                     VCNIDYWDNVGREVGIVAVVSDVYMNPVVDNTAVYFTTDEGSMKSHEARTRDLEGVAS
                     TVWISGTQAGVGADGQVMVAAETAGGTVVDTCYFINSHLPDTIWAAGVPGSILADGAS
                     KAVVWVSAADLNGNFVLDGTPFEADANYLQVEAGTLEDGCNMSSARVKITSSTLSNDF
                     SLTGGQDNGIGAVDIVYYWSGGAMAAYPVSMLTGTAYTANCMVTSGPAKVGPGEQARY
                     SVEIRDRFGNPLGDHTIVLSASGGTVSGISQETNGNGEAGGFVWTAPAAPGDVNLTFT
                     DTDPRGGVILSYKVTVE"
     gene            4668..6326
                     /locus_tag="PKM94_11660"
     CDS             4668..6326
                     /locus_tag="PKM94_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD67332.1"
                     /translation="MSDHASDSARADSSLPEAFFASAAAHAERTAFRAEGGRGRAYTY
                     AEAAAAVRRFARELRAQGVTRGTEVGLLAENRPEWGIAHLAILAAGGTVVPIDGTLKP
                     QEMGYLAGHCRLARVVASRAFDKPARELPGVTVIPLEGVWEAGGPSPDPAESGAAAPA
                     DVAVLIYTSGTTGTPKGVQLTHRNLLANLGAIQDSLRFDESDVFLSVLPLHHTFEMTC
                     GLLTPLMTGCTVVYARALRSKEILEDIGRSRATVMCGVPLLFEKMYHSIRRGIAAAPP
                     GRRAAFRTMYAASGAAWQVGIKLGKKLFAPLRAKAGLDSIRMFVSGGAAMPAAITRFF
                     NLIGFDFMEGYGMSECSPVISVNRPDNIRFGSVGPPLRNVEVRIEGADKSGVGEITVR
                     SEANTIGYRDNPEATAALMRDGWLFTGDLGRFRHGHLWITGRKKNVIVSAAGKNIYPE
                     ELEEQLLASGRVQECVVFGRAKAGKQGEEVRAIIVPALDNLAAELGIDPENPDADRVR
                     QAIEDTVQDVNNRVAAYKRITGCEIREKELEKTSSKKVKRYLYQ"
     gene            6347..6763
                     /locus_tag="PKM94_11665"
     CDS             6347..6763
                     /locus_tag="PKM94_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD67333.1"
                     /translation="MVHAAEIALESAGFCDLVDITAQLSDLVRASGIGSGIVTVFCPG
                     STGGLTTIEFEPGLQKDLPELLERIVPSDRSYHHDRTWHDGNGFAHLRSALIGPDLTV
                     PFTAGKLALGTWQQVVFLDFDNKARRRRLAVQIIGE"
     gene            6779..7786
                     /gene="mtnA"
                     /locus_tag="PKM94_11670"
     CDS             6779..7786
                     /gene="mtnA"
                     /locus_tag="PKM94_11670"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOD67334.1"
                     /translation="MAVEAIQRIGKKIRVIEQSKLPLAFSYLELDDYRDVIAAIRRLD
                     VRGAPAIGIAAAYALALAVEKAADFALSHLERAADEVKAARPTAVNLAWAVDRVMRRV
                     RQAPPLTRREALAILWEEAEAIHDEDRRLCERIGQYGADLIKPGDGILTHCNAGALAT
                     GGKGTALAVIYAAQEAGKRPRVYADETRPLLQGARLTAWELLQAGVDVTLICDSAAAT
                     VLRQGKVQHVIVGADRIARNGDAANKIGTYPLAVLAARHGVPFCVAAPYSTFDENTAA
                     GEQIPIEERAADEVTGGFGRRTAPEGVKVYSPAFDVTPFQLVTYYITDQGIKPGGRAP
                     G"
     gene            7881..8540
                     /locus_tag="PKM94_11675"
     CDS             7881..8540
                     /locus_tag="PKM94_11675"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOD67335.1"
                     /translation="MKARVDSKRQKTAMRACNYELFQFETLAPPPPATVDTKIRQATQ
                     AARPEVAARTDGAAARPTSELPGLDELYRLFDYYNWKYFGGRLPRVKIEYSTRMTCAG
                     SYSPNRRLIKIGRRYHEIFPQDLEDTLKHEMIHIRHYHHDAKFKAEAVRIGASLKARE
                     HPNLRRPPKYVYECPGCGAEYPRQRRLVQASCTYCSANRRYDPRFKLRRKRPRVPRSG
                     T"
     gene            8733..9164
                     /gene="rplM"
                     /locus_tag="PKM94_11680"
     CDS             8733..9164
                     /gene="rplM"
                     /locus_tag="PKM94_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005381375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD67336.1"
                     /translation="MKTFIPKVDPNNRKWWVVDVEGHILGRAAVQVANILRGKNKPIF
                     TPHLDAGDHVVVVNARSIRVTGKNKPRQKTYYRFSGYPGGLRSTSLSTMLRQKPEETF
                     RLAVRRMLPKNRLGRKMIKKLHVYADGEHRHQAQKPEPYKL"
     gene            complement(9291..>9707)
                     /locus_tag="PKM94_11685"
     CDS             complement(9291..>9707)
                     /locus_tag="PKM94_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67337.1"
                     /translation="AKGWPMVTRTFAEPRDFSADDQIEFWLYIQTQNPLPKGRVIGFG
                     AFPADRSYEDWYFPQDLVANQWQQVVVPLKPEHDLTRIGSLSFYIAEAWWQDKDRINF
                     LIDDLRLARHTTATFPDISVIVRASVAWTTSGSRFR"
BASE COUNT         1811 a         3103 c         3338 g         1455 t
ORIGIN      
        1 ccccgccggc ggccgtagcg gccacgaaga ccgaacccgc cccgccggcg ccgagccggg
       61 tgcagacgcc cagcgcgacc ccgccgaccg ccgaggctcc caaagcggcc gccccgccca
      121 agacggcgga gacgcccaag ccggctgcgg gtgcgccgac gccgaaaccc gcgctgccct
      181 cggtcgccaa accggatacg atcacggcgg acgttccgcg gaagccggac atcaagccgg
      241 aaccgccgga gccgatgcgg gtgtccgagg cgaacgcgcc gcaaccgacg ccgaccgcca
      301 agccggcggc gacgccggcg ccgaccgccg gcaagccgtc gactacgccg cccccggcgt
      361 ccgtgaccgc ggccccgaag ccctcgccga cagccccgcc gcctgcgccc aagcccttgg
      421 cgagcacggc gacggtgacc ccgcaggccg cgcccgcgac cccggcgccg accccgccga
      481 ccgcggagga atcggccgaa ccggaggagg atgaggacgt gccggagccg gaggatctcg
      541 aaccggcggt cgagtcgtca gacgacgagt cgccgtccga cgaggccgcg gcccgcggca
      601 ccgcccggtt ccgccgctcg cccaccgatc cgaccaagat caaggggacg ctggtggcgg
      661 agttcccgac gcgcctggtg atcaagtacg aaccgggcgg acggcgcgat ccgttccaga
      721 cgctgatcga cgagacgaag cagtacaaca gcccgatcga acggcgcatc cccaacgtcg
      781 agggtttgcg gctggtgggc gtgctgcagt cgcccctcgg caacagcgcg ctgtttgaag
      841 acaaggacgg gtacggttac atcctccgcg agggggacaa gatccagaag gggtatgtcc
      901 tgaggatcca gaccgaccgc gtgttcttcc agatatttga gtacggctgg agtcgaaccg
      961 tcgccctcaa cctcgaaagc gaaaactgaa aggcaggtgc gcataatgtg gaacgcttgt
     1021 gacaaactca tgcggcggct gctgcccata ctgctggcgg tcgccgtggc cgtgccggcc
     1081 gccgctcaga acgccggcac gcccggagaa ccgatcaaga acctgcagtt tcaggctgcg
     1141 gaaatccggt cagtggtgaa cttcctggcc gactacggcg gcgtcaacgt cgtcgtggct
     1201 cccgaggtcg agggaacggt gaccatccgg ctggttgatg tgccctggcg gactgccctg
     1261 gacatcatcg gccgcaccta caacctcgcc gtcgtcgatg agaaggaggg gtacatccgc
     1321 gtcctcccgg ccgaggacta ccgcaaggaa gtgagcgagc agaaccgcca cctcgccgag
     1381 accaaagaac tcgtccccct gcagaccaag atcgtccaca tttcaaactc gacctcggac
     1441 gacatcgtcg cggccgtgaa gtcgctgttg acggtgcgcg ggcaggcgac gagcgatccg
     1501 cgctcgaact cgattatcgt gcaggaagtc ccgaccaaca tggatgccgt actcgcctat
     1561 atctcccagc tcgaccagcc ggcccagcag atcaagatct cgacccagct gctggagata
     1621 ttcacggaag accttgagga actgggcgtc gactggagcg tgacgggttc gacggcgacc
     1681 gacggcggcc acgtgaacat cgggcagacg ggcgaagtgc ttacggcccg gagcgccgat
     1741 tacgccgggc ggtacaccgt gcacgccctc ggaccgaact ggaacgccga ggcggtcgtg
     1801 aaagccatcg tgaacagcgg gcgcggcaag atcgtggcgc acccggagat caccacgatc
     1861 gacaacaagg aagcccgcat tcagatgggc cagaagatcc cggtcaagca gtttgacgag
     1921 tccggcaacg tcgtgatcgt gttcgaggaa gtcggcacga ttctcacggt gacgccgcac
     1981 atcacggccg agaaccagat cctcatgcac ctgcggccgg agcgctcgac ctacgagttc
     2041 gaccccaacg gcgtggtgat caacaccaac aacgccgaga cccacgtgat cgtcgagaac
     2101 ggccagacgg cggtcatcgg cggtctgacg acggaagaca aggtcgacgc cgaagtgggc
     2161 gtgccgctgc tgaaggacat cccgcttttg ggccggctgt tcaagttcac ccagagccgg
     2221 aacgaggtgc gcgacctcgt gatcttcgtg acgccgacga tcgtggagaa cgatctggcg
     2281 atgaaaaagt aacggagagc cggcgcatca atgtgaaaag gccccaggcg ggtaaccgac
     2341 ggggcctttt cccttggcgc tccgcgcgac cgcgggcgcc ccggccggca ggccgaaagc
     2401 caaacggtaa acagacaagg accgggtgag actatggcca agcgtatctt tcgatgccgc
     2461 gccgccctcg cggcgctcgc ggcggcgtgg gcagccgcct tcctcggcgg ctgttcctcg
     2521 aacaacgacg acggcggcgg tgtggcgctg ccgacggtca cggtgtccgc tagtccctcg
     2581 acggtggccg tcaacgcctc gaccgtcgtg gaagcaaccg tcaggaacgg cggagcgccg
     2641 atcgccgatc tggtggtgac gttcgcggtc caaccggccg gggccggcta tttcacgccg
     2701 cccagcgcgg cgacggacgc cgacggggtg gcggcgacca tattcacggc tgcacaggcg
     2761 ggaacggtcg ccctctcggc cagcacggag atcggctccg cagtgcgcac cgcctccacc
     2821 ggcgtcgtgg tgacctcctc gcaacagggg accggcagcg tcgcaatcac cgtcgcgccc
     2881 gggctgctcc tggccaacgg gacggataca agcctggtga cggtgacggc gcgccaggcg
     2941 ctcggccagc cggtgccgga cgggacgccc attgtatttg cggcggggga gaaattcgcg
     3001 gacaaggacg ccaacggcat ctggacgccc ggaatcgact ctctggtgtt tgacgtaaac
     3061 ggaaacggga actgggacgc aatggggcag attccctcga gcatcccggt gtccggcggg
     3121 accggccagg cgcaggcggt gttcgtggcc ggcaacagcg ccgggacggt gtatgtcaag
     3181 gcgactatcg gggacgagac gatcggcggc agcgcggaca aggcgctgca gctctcgccg
     3241 aatgcgcgca tcagctcgat cttcctgcac tccgacagca tcaacctcgc ggtccgcgcc
     3301 accggcggca tggagaccgc ggtcctgtcc gccaccgcct acgacgggtg gggcaatccg
     3361 gtgcccgagg gactgccgat ttcgttcatt atcaccgatg gtccgggagg cggggagcgg
     3421 ctcgacacgg cggggtacgg cccgtacagc gccgtgacga acagccaggg gacggcgcag
     3481 gcgccaatcc agtcgggcac ggcggccggg acggtgcgaa tccgggcgta ctgcgacacg
     3541 atcctctcca acgccgccca ggttatggtc tcggccggtc cgcccgcgca cctcgtggtc
     3601 ggcgccgacg tctgtaacat cgactactgg gacaatgtcg gccgggaggt cggtatcgtc
     3661 gcggtcgtct ccgacgtcta catgaatccc gtggtcgaca acacggcggt gtacttcacc
     3721 accgacgagg ggagcatgaa atcgcacgag gcccgcaccc gcgatctcga aggggtcgcc
     3781 tcgactgtct ggatttccgg gacgcaggcc ggcgtggggg ccgacggaca ggtcatggtg
     3841 gcggccgaaa cggccggcgg gacggtggtg gacacctgct atttcatcaa ttcccacctt
     3901 cccgacacca tctgggcggc cggcgtgccg ggttcgatct tggccgacgg cgcgagcaag
     3961 gctgtcgtgt gggtctcggc ggccgatctc aacgggaatt tcgtgctcga cggaacgccg
     4021 tttgaggccg acgccaacta ccttcaggtc gaagccggca cgctcgaaga cggctgcaac
     4081 atgtcaagcg cacgggtcaa aatcacctcg agcaccctct cgaacgattt ctcgctcacc
     4141 ggggggcagg acaacggcat cggagccgtc gacatcgtgt actactggtc gggcggggcc
     4201 atggcggcct acccggtctc gatgttgacc ggaacagcct acacggccaa ctgcatggtc
     4261 acctcggggc cggcaaaagt gggtccgggg gagcaggccc gctattccgt ggaaatccgc
     4321 gatcgattcg gcaacccgct gggggaccat acgatcgtgc tgagcgcctc cggcggaacg
     4381 gtcagcggaa tctcgcagga aaccaacgga aacggggaag ccggcggatt cgtgtggacc
     4441 gcccccgccg ccccgggtga cgtcaacctg acgttcaccg acaccgaccc gcgggggggc
     4501 gtcatcctct cctacaaagt gaccgtcgag taaccccccg gcatccgccg ggagagtgac
     4561 atccggccgc cggggcgtgc gctccggcgg ctgcctttgg ggcctttccg gagaggccga
     4621 ccggactttt cccttgcgcg cttcaccgcc gattctacat tggcgacatg tcggaccacg
     4681 ccagcgacag cgcccgggct gatagttctc ttcccgaggc tttcttcgcc tcggcggctg
     4741 cccatgccga gcggacggct ttccgggccg aagggggacg ggggagagcg tacacctacg
     4801 cggaagcggc cgcggccgta cgccggtttg cccgcgaact ccgggcgcag ggcgtgaccc
     4861 gcggcaccga ggtcgggctg cttgcggaga atcgtcccga gtggggaatt gcgcacctgg
     4921 cgatcctggc ggcgggcggg actgttgtgc cgatcgacgg aactctcaag ccgcaggaga
     4981 tgggttatct ggccggccac tgccgtctgg cgcgggttgt ggcctcgcgg gcctttgaca
     5041 agccggcccg ggaactgccc ggcgtgacgg tcatcccgct ggagggcgtg tgggaggccg
     5101 ggggcccctc cccggacccg gcggaaagcg gggcggccgc gccggcggac gtggctgtgc
     5161 tcatctatac ctccggcacg accgggacgc cgaagggagt gcagctcacc catcgcaacc
     5221 tgctggccaa cctgggcgcg atccaggact cgctgcggtt cgacgagagc gacgttttcc
     5281 tgtcggtgct gcccttgcac cacactttcg aaatgacctg cggactgctc accccgctca
     5341 tgaccggctg cacggtcgtc tacgcgcgcg cgctgcggtc aaaagaaata cttgaagata
     5401 tcggccgcag ccgggcgacc gtcatgtgcg gggtgccgct cctgttcgaa aagatgtacc
     5461 attcgatccg gcgggggatc gcagcggcgc cgccgggtcg gcgggcggcc ttccggacaa
     5521 tgtacgccgc ctcgggcgcg gcgtggcagg tcgggatcaa gctgggcaag aaactcttcg
     5581 cgccgctgcg ggcgaaagcc gggctggact cgatccggat gttcgtctcc ggcggggcgg
     5641 ccatgccggc ggccatcacc cgcttcttca acctcatcgg attcgatttt atggaagggt
     5701 atggcatgtc ggagtgctcg cccgtgatct cggtcaaccg gcccgacaac attcgcttcg
     5761 ggtcggttgg gcccccgctg cgcaacgtgg aagtgcgtat cgagggcgcc gacaagtcgg
     5821 gtgtcggcga aatcacggtc cgcagcgagg ccaataccat cggctaccgg gacaatcccg
     5881 aggcgaccgc ggcgctcatg cgcgacggat ggctctttac cggcgacctc gggcgcttcc
     5941 gccatggcca cctatggatc acggggagga agaagaacgt gatcgtgtcg gcggcgggga
     6001 aaaacatcta tcccgaagag ctcgaggagc agctgctggc ctcgggccgg gtgcaggagt
     6061 gcgtggtgtt cgggagagcg aaggcgggca agcaggggga ggaagtgcgg gcgatcatcg
     6121 tgcccgcgct cgacaacctt gccgccgaac tcgggatcga tccggaaaac ccggacgccg
     6181 accgggtgcg gcaggcgatc gaggacacgg tgcaggacgt caacaaccgc gtggcggcgt
     6241 acaagcgaat caccgggtgc gagatacggg agaaagaact ggaaaagacg tcctccaaga
     6301 aggtgaagcg gtacctgtac caatagggag agcgggcgga gatactatgg ttcacgcggc
     6361 ggaaatcgcg ctggaatcgg ccgggttctg cgacctcgtc gatatcaccg cgcaactgtc
     6421 cgatctcgtg cgcgcgtcgg gaattgggtc ggggattgtc accgtctttt gtcccggatc
     6481 aaccggcgga ctgaccacca tcgaattcga accgggcctg cagaaggacc ttccggaact
     6541 gctcgagcgg attgtgccgt cggaccggag ctaccaccac gaccggacct ggcacgacgg
     6601 caacgggttt gcgcatcttc gctcggcgct catcgggccg gatctcaccg ttccgttcac
     6661 ggcgggaaaa ctagcgctcg gcacgtggca gcaggtcgtg tttcttgatt tcgacaacaa
     6721 ggcgcgccgg cggcggctgg ccgtgcagat tatcggcgag tagcgggggc ctgacaacat
     6781 ggcggtagag gcgattcagc ggatcgggaa gaagatcagg gtgatcgagc aatccaagct
     6841 cccgctggcg ttcagttacc tggagctgga cgactaccgg gacgtgatcg cggcgatccg
     6901 gcggctcgat gtgcgcgggg cgccggcgat cgggatcgcg gccgcctacg cgctcgcgct
     6961 ggcggtcgaa aaggcggcgg acttcgcgct ctcccacctc gagcgcgcgg ccgacgaggt
     7021 gaaagcggcg cgcccgacag ccgtaaatct ggcctgggcg gtcgaccgcg tgatgaggcg
     7081 tgtgcgccag gcgccgccgc tgacccgccg ggaggcgctc gcgatcctgt gggaggaagc
     7141 ggaggcgatc cacgacgagg accgccggct ctgcgagcgc atcgggcaat acggcgccga
     7201 cctgatcaag ccgggggacg gaatcctgac gcactgcaac gcgggcgccc tggccaccgg
     7261 ggggaagggc accgcgctcg cggtgatcta cgcggcccag gaggccggca aacggccgcg
     7321 ggtgtatgcc gatgagaccc ggccgctttt gcagggagcg cggctcacgg cctgggaact
     7381 gctccaggcc ggagtggacg tgactctcat ctgcgacagc gcggccgcga ccgtcctgcg
     7441 gcaggggaaa gtgcagcacg tgattgtggg ggccgaccgg atcgcgcgca acggcgacgc
     7501 ggccaacaag atcggcacgt atcccctggc cgtgctggcc gcccggcacg gcgtcccgtt
     7561 ctgcgtggcg gcgccgtatt cgacattcga tgaaaacacg gccgccgggg agcagatccc
     7621 gatcgaggaa cgggccgccg acgaagtgac gggcggattc ggccggagga ccgctcccga
     7681 gggggtcaaa gtgtattcgc cggcgttcga tgtgaccccg tttcagttgg tgacgtatta
     7741 tattactgac cagggaatca aaccgggggg ccgggccccg gggtgatcct cccgtgaacc
     7801 cggacacgag cgccgcccgc ggccgggcgt ttaatgttga gattcggaat gccgatagct
     7861 tatacatcac ggatacacag gtgaaggcga gagtggacag caagcggcaa aaaacggcga
     7921 tgcgcgcgtg caactacgag ctgtttcagt ttgagacgct cgcccccccg ccgccggcta
     7981 cggtcgatac gaaaatccgg caggcgaccc aggccgcgcg ccccgaggtg gcggcgcgga
     8041 ccgacggcgc ggccgccagg ccgaccagcg agctgccggg gctcgatgaa ctctatcggc
     8101 tgttcgacta ctacaactgg aagtatttcg gggggaggtt gccgcgggtg aaaatcgagt
     8161 attcgacgcg gatgacctgt gccggatcgt attcaccaaa ccgacggctg atcaagatcg
     8221 ggcggcggta ccacgagatt tttcctcagg acctcgaaga tacgctcaag cacgagatga
     8281 tccatatccg ccactaccac cacgacgcga aattcaaggc ggaggcggtc cggatcggcg
     8341 cctcgctgaa agcgcgggaa cacccgaatc tccggcggcc cccgaagtat gtctacgagt
     8401 gtcccgggtg cggggcggag tacccccgtc agcggaggct ggtgcaggcc tcgtgcacct
     8461 actgctcggc caaccggcgc tacgatcccc ggttcaagct ccggcggaaa aggcctcggg
     8521 tgccgcgctc ggggacgtga ggggctggga gcggtcctgc tatcggttga ttcccaacaa
     8581 gttgcgtctc gcccagtgga actgccccgg ggaggcggcg gtccgaaggc caggccaatt
     8641 attttgctga accgtattga cattgacgcg gaatgcagta tacttgcagg ctgtcttgac
     8701 ggtgaaaact atgaggttat gagaatatag agatgaagac gtttattccg aaagttgacc
     8761 cgaacaaccg gaagtggtgg gtggtggatg tcgaagggca catcctcgga cgggcggcgg
     8821 tgcaggtggc gaacatactg cgcggcaaga acaagcccat cttcacgccc cacctggacg
     8881 ccggcgacca cgtcgtcgtg gtcaacgccc gcagcatccg ggtgaccggg aaaaacaagc
     8941 cccggcagaa gacgtactac cggttttcgg gctatccggg cggactgcgg agcacgagtc
     9001 tctccaccat gcttcggcaa aaacccgagg agacgttccg gctggcggtg cggcggatgc
     9061 tgccgaaaaa ccggctgggc cggaagatga tcaagaaact gcacgtgtac gccgatggcg
     9121 agcaccgcca ccaggcgcag aaaccggaac cttataagct gtagagtttg acgtttgccc
     9181 atggagaaga ctgagtacgc cgcgacggga cgtcgcaaat gcagtgtcgc ccgcgccgtg
     9241 atcaccaacg gagacggcgg tttcacgatc aacgggtccg acatcgccgc ctatctgaaa
     9301 cgcgatccgc tggtggtcca cgccaccgag gccctcacga tcaccgagat gtccgggaag
     9361 gtggcggtcg tgtgccgggc cagccgcagg tcgtctatga ggaagttgat gcggtccttg
     9421 tcctgccacc aggcctcggc gatgtagaag ctcaggctgc cgatccgcgt caggtcgtgc
     9481 tcgggcttta gcggcacgac gacctgctgc cactggttgg cgaccaggtc ctgcgggaag
     9541 taccagtcct cgtacgacct gtccgccggg aaggccccga agccgatgac gcgccccttg
     9601 ggcagcggat tctgggtctg gatgtacagc cagaactcga tctggtcgtc ggccgagaag
     9661 tcccgcggct cggcgaaggt ccgtgtgacc atgggccagc ccttggc
//
LOCUS       DAOPOE010000106         9489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8158_length_9489_cov_6.447106, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000106 DAOPOE010000000
VERSION     DAOPOE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_9489_cov_6.447106"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            660..1073
                     /locus_tag="PKM94_11690"
     CDS             660..1073
                     /locus_tag="PKM94_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD67338.1"
                     /translation="MRERSDVLILGPPGVGKSHLVQAIGHQAARMGFTVLYRSIFDCL
                     GELIPAENFGERVTTMNRYLKADLLIIDEMGIKKLPKQSGEYVFEIVMRRHELRSTIM
                     TSNRPLDNWGKLIGDVPTAAAILDRLLHRAEIAAS"
     gene            complement(1239..1697)
                     /locus_tag="PKM94_11695"
     CDS             complement(1239..1697)
                     /locus_tag="PKM94_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67339.1"
                     /translation="MCLALVLVAQPLLAQEKPIQLSLFSPIQIVPEEKAIAGLRLSLL
                     YGKNQYVSGLDWGLVSHCTAGISKGVQFGLVGLIDADLSGWQSTAVNITKGTCEGFQW
                     GMVNYAGHASGLQLGFVNYAVTMRGLQIGLVNIIKQGGQFPVFPIVNWSF"
     gene            2139..3007
                     /locus_tag="PKM94_11700"
                     /pseudo
     CDS             2139..3007
                     /locus_tag="PKM94_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2417..2533
                     /locus_tag="PKM94_11700"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3054..4028)
                     /locus_tag="PKM94_11705"
     CDS             complement(3054..4028)
                     /locus_tag="PKM94_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD67340.1"
                     /translation="MRQWIYRIVKVALVLLAVGLAGRQLWVNWEAVREYDWQINFGWL
                     GLSVAAHLFTLLLFSIVWCRLILGFGHRLKIRYSFKLAYIATFGRYIPGRIWPLLAMA
                     YLARRLGIPEQHSLTSWGLALIFTLTAAFAVTAAGIGLVPSQVGLVFQGYLGAGVWAA
                     GVVLALASLALIYFPDRTLVVFNFLLRLIGRKPLRFRLRPAIAFQVLGGYAACWISYG
                     VSFWLFLKAVMGATPVPIVSAITIFVIAYQMGYLAVFSPGGIGVRELALIGLLHPFLG
                     PISAAVAVAARLWNMVVEIIAVIIAWRVPMPAENSERDLTGLPPEPPA"
     gene            complement(4246..6108)
                     /locus_tag="PKM94_11710"
     CDS             complement(4246..6108)
                     /locus_tag="PKM94_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOD67341.1"
                     /translation="MRIVAAVAALTAVVAAGAAQAGLTDPYAILERSVAASGGLDNIR
                     SHETSYAEGTIEIVGSGLAGPFRQWQRRPELQRLEVELGPLAITQGNNGEVAWTVDQN
                     GKVQIHRDELTVGQEKADSLMAEFAHLDRGSAVFAVTYDGLDTAAGAACYVVTITNTL
                     SLDTTRLYIDTASFRQRKSAALTASGQTITVYDDFRTVAGVEMPFSQRQVTLPVNMVQ
                     NIVMTKYEANVPIEPAMFNPPSEDVSDVRFADGDRAEDIPFRFIENHIYVPVTVKGKE
                     RLWILDSGAEMTVVEKRFAEEIGLETQGNITGKGAGNLVDVTFTTVPMLSIDDRVLVD
                     EQKVAVIDLYWFFQEALGLEIAGILGYDFLSRVVTRVDYAGERLSFYEPGNFAYAGPG
                     VVLDLPISQRKSFEPEVIVDGVLRGNWYLDLGAGGLSFGYPYARANGLLDRPGVDHLA
                     YGAGGSFPVRAVRFDSLAFAGFTVRDEIIDIPAAEVTGAFAEGTVAGNLGNSLFRRFV
                     LFLDYSREQLIVEKGVDFDRAFPRDNSGMQVRYNRDQRISVLFVAPGTPAATSGLQVG
                     DLLRSANGIDISNLDGVVAVRRLFQAGPGTAYDLVVERDGRPIQVRLTLRDLYQ"
     gene            complement(6245..7147)
                     /locus_tag="PKM94_11715"
     CDS             complement(6245..7147)
                     /locus_tag="PKM94_11715"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOD67342.1"
                     /translation="MSRTARITIAALAMVLGAAGAVAAQEATVDSTFIWKRALAVDLT
                     ATQTAYSDSWTGGEAGAFSWVGNAAGFAERNLNPWFNLRSQVKLSFGQTTTQDEETKD
                     WSKPKKSTDLIDWETVGRFLLNQYVDPYAAFRLESQFYDGSNAKKKLYLSPLKLTESA
                     GLAHRFYEKSNDHITTRLGGAYRQIITRFIADTVTLATDSRTVSDAGLESVTDAVLTL
                     SEKMQYTGKLTLFKALYNSEDDALGADDDWKAVDVRWENAVTAQVSKIIAVSFLFDLR
                     YDKEIVDKAQIKETLGLGFLFKLI"
     gene            complement(7162..7977)
                     /locus_tag="PKM94_11720"
     CDS             complement(7162..7977)
                     /locus_tag="PKM94_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD67343.1"
                     /translation="MDRVNDRLLEWGAIYGTKIIGALVILIVGYLLTKFLTAAVRRIT
                     TRAQVDAALVKFLATVTKVMLLVFVILAAMNSLGVQTTSFIALIGAAGLAVGLALQGS
                     LANFAAGVMLILFRPFKGGDYVETAGVAGTVEEIHIFHTVLRTPDNKKVTVPNSAVTG
                     GNIINYSAHDTRRIDMVFTVGYRDDLRLAKRTLEEILAADPRVLKDPPPTVAVGQLAE
                     SAVNIVVRPWVRTEHYWPVLFDVHERVKERFDEQGVTIPYPQRDIHVHQTRVS"
     gene            8149..9300
                     /locus_tag="PKM94_11725"
     CDS             8149..9300
                     /locus_tag="PKM94_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HOD67344.1"
                     /translation="MRPLTAHRTTASLVTLLAPLILASCSEAGPAARATAPDGDLGKI
                     SLPPGFSISYYADRMPGARSMRLGDNGTLFVGTRGDKVYALRDVDGDYRADSLYVIAT
                     GLDMPNGVAFRDGSLYVAEVSRVIRLDGIESRLSDPPTPAVVTDTFPSDRAHGWKFIG
                     FGPDGRLYVPVGFPCNVCEPDEDICGTIVRMDPDGGRAETFARGVRNTVGFDWEPTSG
                     VLWFTDNGRDRMGDDIPPDELNRAPSAGMHFGFPYCHAGDIPDPQFGQGADCDTFAAP
                     VRKLGPHAAALGMRFYTGAMFPEKYRGQIFIAEHGSWNRSTPLGYRVMLVTHDGEGHA
                     TSYEVFAEGWLEDNGARWGRPVDVLVMPDGSLLVSDDEAGAVYRITYGR"
BASE COUNT         1781 a         2828 c         3244 g         1636 t
ORIGIN      
        1 ttcggattga catccggatg gagaacggtt atgaagaggg ttgcacacgg ggagcttttc
       61 tatccttgga tcgaactgcg tttccgagtg cggcggtccc gatctcacga ccatgcgccg
      121 taacctccaa tttgataaaa agttagcgtg atccattggc aggatttgag gcgttctttg
      181 gaagtctcat tcgctgcccc caagggaagg gccgctgaaa tctcgggaat tgcccggatg
      241 atctgcaata acgtgactta gataaagggg cagactcaga tagcacttct atcgagccgg
      301 tggcgagcat atcgtcgtga gcgggggcca ttttacggac tccccctccc ccactattct
      361 gccgcactga ttgaacaacc ggaatctggg aggcaacgtg gcgcgtgacg ggataccggg
      421 attgcactcg tttgaaggca gtcatcaaga tacctcaaaa gaggctcaag tcaagtcgca
      481 ctatcgaccg cccgccgcag gtgaacaatc atctctgaaa tgcagccttc tgccccgtta
      541 gatatgtgga cagtgattag aacgaagatt ccgattccct gaggccgctc tcggactcgg
      601 gcggggaaga aaggcgagat tatcatgaag agaatagtct taacactgct ggtcgcttca
      661 tccgcgaacg gagcgacgtc ctcatcctgg gtccccccgg cgtcggcaag tcgcacctgg
      721 tccaggcgat cggccatcag gcggcccgca tgggcttcac cgtcctgtac cgctccatct
      781 tcgattgcct aggcgaactg atcccggccg agaacttcgg ggagcgtgtc accaccatga
      841 accgctacct caaggcggac cttctgatca tcgacgaaat gggcatcaag aagctgccca
      901 aacagtccgg ggagtacgtc ttcgagatcg tcatgcgacg gcacgaactg cggtccacca
      961 tcatgacctc caatcggccc ctggacaact ggggcaagct gatcggcgat gttcccaccg
     1021 cggccgcgat cctcgatcgc ctgttgcacc gggcggagat cgcggcatca tgataccgat
     1081 gccagacaga aaacctggcc ggttttcacc cggcccgcag accgattctg cccgttggtg
     1141 cggaccagcg gaaaagcctc atcgcgctgc ttgagtgcag cgggatcacg gcctgaaaca
     1201 cgcgcggctg cccgcgcttc agccggcgtc gcagatgtct agaacgacca gttcacgatg
     1261 ggaaagacgg gaaactggcc cccctgcttg ataatgttta ccaggccgat ctgcaggcct
     1321 ctcatagtca cggcatagtt gacgaacccc agttgcagac cgctagcgtg gccggcatag
     1381 ttaaccatac cccactggaa cccttcgcag gtacccttgg tgatgttgac ggccgtactc
     1441 tgccagccgc tgaggtcggc atcgatcaaa cccaccagcc cgaactggac gccctttgag
     1501 atcccggcag tgcagtgact gaccagcccc cagtccagac cgctgacgta ctggttcttg
     1561 ccgtaaagca ggctgagccg cagtccggcg atcgctttct cttcggggac aatctgaatc
     1621 ggcgagaaga gagaaagctg gatcggtttt tcctgcgcca gcagcggctg ggcgacgagg
     1681 acgagagcaa gacacatcac cccaaacagg ctgcgattcg tcttcgacat ttgatcctcc
     1741 aatctgctga atgcctcggc gacggacaac cttgctgcac atatacgcaa tcgggagggc
     1801 atagtcaaac gaacttggat ccgcagagag cccgtcttgc gccaatgctg tcacgtgcgg
     1861 cgaggggctg ccgaggcccc cggatgggag aaaatgcttc actcgcagtc cggtcggcgt
     1921 gcacccgaac gactctcagc ggcgccgatt cggagatcgc agggaagggg gtgtcgccca
     1981 atatgcgatc aatgtggcgg aggggttggg attctgttgg cggtgtcctg gtatgtcgtt
     2041 gcgtgcgaag aaggcacgcc gaaagttatt atatcggatg cgattaggtg aaccgccccg
     2101 gggttgccgg agacaggata atgtgagaga tcactgttat ggcaagacca agcaggtatt
     2161 cacgggaggt ccgggaacgg gccgtgcgga tggtcgagga gcatcgggag gagcatagct
     2221 cccagtgggc ggccatcgag tcgatttccc agaagatagg ttgcaccgcc gagactctgc
     2281 gaaagtgggt tgttcaggct cagcgggacg cgggtcatgg gccgggtctg acgacctcgg
     2341 agcgtgagcg gctcaagcag ttggagcggg agaaccgtga gcttaagcgg gccaacaaga
     2401 tactgcggaa ggcgtcggct tgttttgccc aggcggagct cgaccgcaaa ccgaggtgat
     2461 ggtgtcgttt atcgacgagc atcgggacgc ccacggggtc gagtcgatct gcgccatgtt
     2521 gccgatcgcc ccgtcgacgt actaccagcg gaaggggcag gaggcacatc cggagcgttt
     2581 gtcggcccga tcgcggcggg agctgaagcg gcaaccatag atccagcggg tgtgggagga
     2641 gaacttccag gtgtacgggg cgcggaaggt atggcggcag ctgaatcgtg aagggataga
     2701 ggtggcgcgg tgtacggtgg agcggttgat gcgggatttg ggtctggccg gtgcggttcg
     2761 tggtcggaag tgtcggacca caattgccga tgatagcgcg gtgcggccgg cggacctggt
     2821 gtgccgggag gttacggcgt tacgtccgaa ccagttgtgg gtctcggatc tgacgtatgt
     2881 ggcgacctgg caggggtttg tgtatgtggc gttcgtgatc gacgtcttct cgcggatgat
     2941 cgtgggctgg cgggcgtcgc gatcgttgcg cactgatctg gccctggacg ctctggagca
     3001 ggcgctgggg acgcgcccgg caattccgac ggcggcgcgg gcaggccccg cggtcaggcc
     3061 ggcggttcgg gggggagacc ggtaagatcc cgctcagagt tctccgccgg cataggcacg
     3121 cgccaggcga tgatcaccgc aatgatctcc acaaccatgt tccacaagcg cgccgcgacc
     3181 gccaccgcgg ccgagatcgg gccgaggaag gggtggagca gtccgatgag agcgagttcg
     3241 cgcaccccga tgccgccggg ggaaaagacc gccaagtacc ccatttggta cgcgatcacg
     3301 aagatcgtga tcgccgacac gatcggcacg ggggtcgccc ccatcacggc tttcagaaac
     3361 agccagaatg acacgccgta ggagatccag caggccgcgt agccgcccag cacctggaaa
     3421 gcgatggccg ggcggaggcg gaaccgcagc ggcttgcgcc cgatgaggcg caggaggaaa
     3481 ttgaacacga cgagggtccg gtccggaaag tagatgagcg cgagcgaggc cagcgcgaga
     3541 acgacgccgg ccgcccacac gccggcgccg aggtacccct ggaacaccag ccccacctgc
     3601 gacgggacga ggccaatccc cgccgcggtg acagcgaagg ccgcggtgag agtgaagatg
     3661 agcgcgagtc cccaggacgt gagcgaatgc tgctcgggaa tgcccaggcg ccgcgcgagg
     3721 taggccatcg ccaaaagcgg ccagatgcgg ccggggatgt accggccgaa agtggcaatg
     3781 taggcgagct taaaactgta gcggattttc agccggtggc cgaacccgag aatcaggcgg
     3841 caccagacga tcgagaaaag cagcagcgtg aacaggtggg cggccaccga gagaccgagc
     3901 cagccgaaat tgatctgcca atcgtactcg cgcaccgcct cccagttgac ccagagctgc
     3961 cgcccggcga gaccgaccgc gagcaggacc agggcgacct tgacgatcct gtaaatccac
     4021 tgacgcacac ggcgggccgg cggcggcgcg ccgggcggcg gggtgatcgg ggatgaactc
     4081 accggacgga ttggtctcca cgtcaaagct tgatgtcggc caataatata gcggggggca
     4141 gtccggcaat caaggttgaa gatcgcgccg gccgcggatg cgcggcgcct ccgcccggca
     4201 cccgctgcgg ccgccccggg gccggggcgg ccggcaattg gactgctact ggtagaggtc
     4261 gcgcagggtc agacggacct gaatcggccg gccgtcgcgc tcgacgacca gatcgtacgc
     4321 cgtgccggga ccggcctgga acaggcggcg caccgcgacg acgccgtcga gattcgagat
     4381 gtcgatgccg ttggcgctgc gcaggagatc cccgacctgc aggccggacg tggcggctgg
     4441 cgtgccgggg gcgacgaaca agacggaaat gcgctgatcg cgattgtagc ggacctgcat
     4501 gccgctgttg tcgcgcggaa aagcgcggtc aaagtccacg cccttctcga caatgagctg
     4561 ctcgcggctg tagtcgagga aaaggacgaa gcgccggaag agggaattgc cgaggttgcc
     4621 ggcgacggtt ccttcggcga aagcgccggt gacctcagcg gccgggatgt cgatgatctc
     4681 gtcgcgcacc gtgaagcccg cgaaagcgag ggaatcgaag cggaccgcgc gcacggggaa
     4741 agacccgccg gcgccgtagg cgagatggtc gacgccgggc cggtcgagca gcccgttggc
     4801 ccgggcgtac gggtagccga aggacagccc gccggcgccc aaatccagat accaattgcc
     4861 ccgcagcaca ccgtcgacaa tgacttccgg ttcaaagctt ttgcgctggg agatcgggag
     4921 atcaagcaca actccgggtc cagcgtaggc gaaattccca ggctcgtaga aggacagtct
     4981 ctcgcccgcg tagtcgactc tcgtgaccac gcgcgagagg aaatcgtagc cgagaatgcc
     5041 ggcgatctcc agtccgagcg cctcctggaa gaaccagtag aggtcgatga cggcgacttt
     5101 ctgttcgtcg accaaaacgc ggtcgtcgat actgagcatc gggacggtcg tgaaggtgac
     5161 gtcgacgaga ttgcccgccc ctttgccggt gatgttcccc tgcgtctcga gcccgatttc
     5221 ctcggcgaag cgcttctcga ccactgtcat ctccgcgccc gagtcgagga tccagagacg
     5281 ctctttgccc ttgaccgtaa ccggcacgta gatgtgattt tcgatgaacc ggaacgggat
     5341 atcctcggcc cggtcgccgt cggcgaagcg cacgtcggag acgtcctcgc tcggcgggtt
     5401 gaacattgcc ggttcaatcg gcacattcgc ctcgtacttg gtcatcacga tgttctggac
     5461 catgttcacc gggagcgtga cctggcgctg ggagaacggc atctcgacgc ccgcgactgt
     5521 gcggaaatcg tcgtagacgg tgatcgtctg cccgcttgcg gtgagagccg ccgatttccg
     5581 ctggcggaag gaggcggtgt cgatatagag cctcgtggtg tcgagcgaga gagtgttggt
     5641 gatggtgacg acgtagcagg cggcgccggc ggcagtgtcg aggccgtcat aggtcacggc
     5701 gaacaccgcg ctgccgcggt cgaggtgggc gaactccgcc atgagactgt cggccttctc
     5761 ctggccgacc gtcagctcgt cccggtgaat ctgcactttg ccgttctggt cgacggtcca
     5821 ggcgacctcg ccgttgttcc cctgggtgat ggcaagtggt cccagttcga cctcgaggcg
     5881 ctgcagttcg gggcgacgct gccactggcg aaacggtccg gcgaggccgg agccgacgat
     5941 ctcgatcgtc ccctcggcgt agctggtctc atggctgcgg atgttgtcga ggccgccgga
     6001 ggcagcgacg ctgcgctcca ggatggcgta gggatcagtg aggccggcct gcgcggcgcc
     6061 ggccgcgacg acagccgtca gggcggcgac ggcagcaaca attcgcatga aggcacctcc
     6121 atgatgagtc ggtcggtttg tgcgtcttga cctgtcagta cgggagagcc gacgctttgt
     6181 tcggcctcgc acccacaaat ccccgggccg gggcccgggg atgtgaaggg atcgggttcg
     6241 cgaactagat cagcttgaac aggaacccga gtcccagggt ctccttgatc tgggccttgt
     6301 cgacgatctc cttgtcgtag cgcagatcga agaggaagct cacggcgata atcttgctga
     6361 cctgggcggt gacggcgttt tcccagcgga catcgaccgc tttccagtcg tcgtcggcgc
     6421 cgagcgcgtc gtcctccgaa ttatacagcg ccttgaacag cgtgagcttg cccgtgtact
     6481 gcatcttctc gctgagcgtc agcacggcat cggtcaccga ttccaggccg gcgtcggaga
     6541 cggtgcggga gtcggtggcg agggtgacgg tgtcggcaat gaagcgggtg atgatctgac
     6601 ggtaggcgcc gcccaggcgc gtggtgatgt ggtcgttgct cttctcatag aaccggtggg
     6661 ccagtccggc cgactcggtc agcttgagcg gcgacaggta gagcttcttc ttcgcattgg
     6721 agccgtcgta gaactgcgac tcgaggcgga aagcggcgta gggatcgacg tactggttga
     6781 ggaggaagcg gccgaccgtt tcccagtcga tcaggtcggt ggatttcttc ggcttggacc
     6841 aatccttggt ctcctcgtcc tgagtggtcg tctgcccgaa cgagagcttg acctgcgagc
     6901 gcaggttgaa ccacgggttg aggttgcgct cggcgaaacc ggcggcgttg ccgacccagc
     6961 tgaacgcgcc ggcctctccc ccggtccagg agtcggaata ggcggtctgc gtggcggtca
     7021 ggtcgacggc gagcgcgcgt ttccagatga aggtcgagtc gacggtcgcc tcctgcgccg
     7081 cgactgcgcc ggctgcgccg agaaccatgg cgagcgcggc aatcgtgatc cgggcggtac
     7141 gggacatacg gataacctcc gtcagctaac acgggtttga tggacgtgaa tatcgcgctg
     7201 cgggtacggg atcgtgaccc cctgctcgtc gaaccgctcc ttgacccgct cgtggacatc
     7261 gaacagcacc ggccagtagt gttcggtgcg cacccagggg cggacgacaa tattcaccgc
     7321 gctctcggcc agctggccga cggccacggt cgggggcggg tctttcagga cgcgcgggtc
     7381 ggccgcgagg atctcctcga gtgtccgctt ggcaagccgg aggtcgtcgc ggtagccgac
     7441 tgtgaacacc atgtcgatcc gccgcgtgtc gtgggcggag tagttgatga tgttgccgcc
     7501 ggtgacggcg ctgttgggca ccgtcacttt cttgttgtcg ggcgtccgga gaacggtgtg
     7561 gaagatgtgg atctcctcga ccgtaccggc gacgccggcc gtctcgacgt agtcgccgcc
     7621 cttgaagggg cggaagagga tgagcatgac gccggcggcg aagttcgcca gcgagccctg
     7681 cagcgcgagg ccgacggcca gaccggccgc gccgatcagg gcgatgaacg aggtcgtctg
     7741 caccccgagg ctgttcatcg ccgcgaggat gacgaagacg aggagcatca ccttggtgac
     7801 ggtcgcgagg aacttgacca gcgcggcatc cacctgggcg cgggtcgtga tgcggcggac
     7861 cgccgcggtg aggaatttcg tgagcaggta gccgacgatc aggatgacga gggcgccgat
     7921 gattttcgtg ccgtaaatcg cgccccactc aagcaaccgg tcattcactc tatccataac
     7981 ccgcaccttt ccgtttcgca ttccgaggct gccgggaaac taccatgcgc cgtacggcct
     8041 cgtcaagcgt ttccggcggc ccgccggtcc ggcgggaaaa tgacaggaac cgtcgcccgc
     8101 gggcgcggtt gtacagacgg agacgatcaa ccgcccggag gataccgcat gagaccgctc
     8161 accgcgcacc gcacgaccgc ctcgctcgtc actctgctcg ccccccttat tctcgcctcc
     8221 tgctcggagg ccggcccggc cgcccgcgct actgcgccgg acggcgacct ggggaaaatc
     8281 agtctgcccc ccggattttc gatcagctac tacgccgacc ggatgccggg ggcgcggtcg
     8341 atgcggctcg gggacaatgg cacgctcttt gtcgggacgc gcggggacaa ggtgtacgcg
     8401 ctgcgcgacg tcgacggcga ctaccgggcg gactcgctgt atgtgatcgc gaccgggctc
     8461 gacatgccca acggcgtggc gttccgggac ggcagtctgt atgtcgccga ggtgagccgg
     8521 gtcattcgcc tggatgggat cgaatcgcgc ctgagcgatc cgccgacgcc ggcagtggtc
     8581 accgatacat ttccgagcga ccgggcgcac gggtggaagt ttatcggttt cggccccgat
     8641 ggccggttgt acgtgccggt cgggtttccg tgcaacgtgt gcgagcccga tgaggacatc
     8701 tgcgggacga ttgtacggat ggatcccgat ggggggcggg ctgaaacctt cgcgcgcggc
     8761 gtgcgcaaca cggtcgggtt cgactgggag ccgaccagcg gcgtgctctg gtttaccgac
     8821 aatgggcggg accggatggg cgacgacatc ccgccggacg aattgaaccg ggcgccctcg
     8881 gccgggatgc atttcgggtt cccctactgc catgccgggg acatcccgga cccgcagttc
     8941 ggccaggggg cggactgcga caccttcgcc gcgccggtgc ggaaactcgg accccacgcg
     9001 gcagcccttg gcatgcggtt ctacaccggg gcgatgtttc cggagaagta tcgcggccag
     9061 atattcatcg ccgagcacgg ctcctggaac cggagcacac cgctcgggta ccgggtgatg
     9121 ctggtgacgc acgacggcga ggggcacgcg acctcgtacg aagtgttcgc ggaagggtgg
     9181 ctggaggaca acggggcgcg gtgggggcgg ccagtggatg tgctggtgat gccggacgga
     9241 tcgcttttgg tttcggacga cgaggccggg gcggtgtaca ggattacgta tgggaggtga
     9301 ggggatgccg gcgccccaag ggaccggcga ccgcggcgct gtatcctcct tgccgggatg
     9361 acgcgggatt gccacgctgc cgcgccccgc cgccctctcg agaatctccc ctcagtacgg
     9421 gtagaagaca aagaacagcg ccgacatcac ggcgagcacc cacatccccg gatgcacctc
     9481 gcgcacgcg
//
LOCUS       DAOPOE010000107         9474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8181_length_9474_cov_5.518845, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000107 DAOPOE010000000
VERSION     DAOPOE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_9474_cov_5.518845"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKM94_11730"
     CDS             complement(<1..665)
                     /locus_tag="PKM94_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOD67345.1"
                     /translation="MNSKAAPECSVRKGGSHWTISFSAMASPCEILIRCRRRSEAEHV
                     ASLAAAETRRIERKLSRYRDDNIIHAINAGTGAAVEVDDELARLLTYADQCYRLSGGL
                     FDITSGVLRRAWKFDGSTASPDRDLIESLLGRVGWEKVEWDGRRLRLQPGMEIDLGGL
                     GKEYAADRAADLAFRASGAPLMANFGGDIRALSADPDSPPWVVGIEDPLREGTAVGRI
                     DLS"
     gene            complement(655..2004)
                     /locus_tag="PKM94_11735"
     CDS             complement(655..2004)
                     /locus_tag="PKM94_11735"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HOD67346.1"
                     /translation="MRMQLTNRPPLRGALSALTAALLGTGAAASEGRNRVESSILLYS
                     EVDRVQAAEAIIGLGRALKGDRMLSARLTLDGLTGASPNGATPSARVQTFARPSGEGG
                     YSVKPGEIPLDDTFKDTRYSFDGSLAQPLDRLTTIVLGAHYSGEHDYTSLGASVGLSR
                     DLNRKNTTLSASAAYSRDRVRPEGGPPDPLSSMSARADDDDDDDAGERELAAQDDDGD
                     DDDDEDRESDAGEGKNVYDVVFGLTQVLNRQTVLRLNYSYNHSSGYLNDPYKIVSVVQ
                     GPGGPDAGEPVDYLYEARPDGRAKQAVFAQLRRYLSGHTIDLSYRYFWDDWGVTSQTV
                     ELFYRLPVKAGHALQPHVRWYRQTEADFYRVFLVEAAPAPAHASADYRLAPFHAVTAG
                     LQYLFPVGQGVQVSLGGEYYHQAGDISPPRSMGVLSTYELFPDMDAVMVRAGFDYEF"
     gene            complement(1989..2222)
                     /locus_tag="PKM94_11740"
     CDS             complement(1989..2222)
                     /locus_tag="PKM94_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HOD67347.1"
                     /translation="MRRLRFFVLSAGAFALAAVLSGCAAVGVQVWERDVLSKPEMQMD
                     SHALDRALDDHIYFSKEASSGGRSFGGGGCGCN"
     gene            complement(2219..2725)
                     /locus_tag="PKM94_11745"
     CDS             complement(2219..2725)
                     /locus_tag="PKM94_11745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD67348.1"
                     /translation="MIAFVRPRGVGVLLAALVLSAAAVPCLAAPPDSLNALQTALADS
                     LDLNGKVVYVDFWASWCVPCRHSFPWMQALSDKFRNRGLEIIAVGVDQDHRAAVKFLE
                     ENRTAFPIVFDSTGRLAKLYELEAMPTSFLYDRSGRLVSTHRGFRPDETDSLEAAIVR
                     LLDKGGAQ"
     gene            complement(2961..4067)
                     /locus_tag="PKM94_11750"
     CDS             complement(2961..4067)
                     /locus_tag="PKM94_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67349.1"
                     /translation="MSHVVGSRWAALILPAALVVAFSARSEVISYQGRLTDPDGLPIA
                     DSLYSMTFSLYADSTGGAPLWQETATVPTYRGLFVHLLGAVSDLPSALFADHSALFLQ
                     VTLGGADLAPRTRLAAAPSAMVARQLYLTDAAGGLLGRTVNDSGGAFILYDTAGALGI
                     ALRGGRASDSAVVFPDSAINADEILDEPGIVNGISSDIITLSTAEMTDLVTVEITIPY
                     DGLIVLYGKCYAVLSGTTGPNGAQVQIDAVEGGLPIFPYYTQAGLSGFVNTGANYFPA
                     VVTRTYYKEAGTYEFRLEAKATQPSPALAQTWDHVLTALYFPTAYGYTTVSTKTAGGV
                     ATPPPGSDSRGSGRLGETIRDDAPLVGDSARTDR"
     gene            complement(4189..7311)
                     /locus_tag="PKM94_11755"
     CDS             complement(4189..7311)
                     /locus_tag="PKM94_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD67350.1"
                     /translation="MRRIVAAGLGVVLLAGLLGAASGLIGPAGDPFDPTLLRSRPLQS
                     GDSAGDTSLSITVERSGFPLIRVYADVLDESDGPVAGLAGPDFAVTQDGAPVSFSLLP
                     AAEIGCPAAFCLVFDVSLSMAGEPLASARRAAHRFVDRMGPFDRAAVVSFADCAMVNA
                     PFTDDRAALHAAVDSLVALGLTASFDGVYLGVQLAAAQPGNRAVIAFSDGCDNNSAYC
                     DLPADGWADGYEDDSALVCDLARAAAVSVYTLTFGTLDSVCGDAMEAFARGSGGLHRY
                     ASDADAVDSVFAAIQHAVCSRYLITYFSPDSAADHDFHLVEVCATLGGAVCCDTAVYR
                     EPAVPTIHLAPTTLQLSDTCQPAAEDITIEALAEDPGGAGLQDVRLFYRQVGAAPEPY
                     RDVAMIAVGGDRYAVVLPAGAWPPDAAGFDYFISAANGDVTVTLPAVDPFQLPFAVSF
                     CPNQPPEISAAAAVCVDSGALLSAVVRDDPDGVARAIAVVFDGGLPSVYDMLPVGGDT
                     FAVALPRVPQNARLLLSVRAWDNLGLSRTTVPLDWHCGLLPEDNWIALVCSGPVFAGR
                     SLRPGDSLRVFDPDGVLSGSAVVGDDSTFGSLRVVGDDPSTPLVDEGAEPGDLLTFTL
                     NAVEVLLTPPVFFRAPGDTIEVCDRVSCRHFALDPGWHLISWNRAFRADIDSLVSLLG
                     GAACVDVVLGFERGGLTYDPNLVEFSTLFEADYHRAYWLRLACPATFDICGGAIAPHD
                     TLPVRRGFNLVPCWPDSSLPVETAWASILEFTDVGLGFDGGSRIWLPDGDSANTLTEL
                     APGFGYWVHADRDGYLVYPGWSSPAGRPLPDEPFGLAAPAVPATQTWMSVYGVGPACD
                     GLPVADGAVIEFHTSGGVLCGRGAIEDGILPFTPVYGASTAAPLYPRPGDTLTVWVDG
                     SPVLPPLVFQGHGSRVPLPALRSSASGPDSPRLPSAYVLRQNYPNPFNPQTVISFDLP
                     LPGRVALTVYNILGRETAVLADQRYPAGTHAVIWDGLDKDGRPVGSGVYLYRLEAGGA
                     VLSRKMLLLR"
     gene            complement(7462..8574)
                     /locus_tag="PKM94_11760"
     CDS             complement(7462..8574)
                     /locus_tag="PKM94_11760"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HOD67351.1"
                     /translation="MHLLALEPYYAGSHRAFVDGWIAGSRHRWTLLTLPGYKWKWRMR
                     HAPLTFCDEIAARRRDGERWDGLFATDMVDLAQLLGLARAALGNLPAVVYFHENQLTY
                     PSLHAGERDYHFAMTNFSTAAVAAGVWFNSDFHRREFLEGLRSFFRRMPDYPPLAWVD
                     RIAARSEVYPQGIAPPRPLSARPPGPLRILWAARWEHDKNPEDFFAAMRALRSRGVDF
                     RLSVIGEQFSEVPAAFAEARREFAPLIDRWGYQPDRADYERALSAADLIVSTARHEFF
                     GVAIVEAVAAGIRPVLPQRLAYPEIFPPQSFPDWYYDGTVAGLVDKLVMLARRFAETG
                     SLRSPSLPAGASAAERFFWPVLAPRLDDALEAALRR"
     gene            complement(8695..9333)
                     /locus_tag="PKM94_11765"
     CDS             complement(8695..9333)
                     /locus_tag="PKM94_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67352.1"
                     /translation="MRKNAPLLIALFLATATLAPAVDFGFGLQVDDEGRKSFYLAVSN
                     HYQVPEKEIVVVRERHIPDEEMPVVFFLAARCNASPATIIDLRLGGKSWMDITLQFGK
                     TAEIFYVPVEDVSGPPYGHAYGHFKKHPKNRWGEIRLSDDDVVNFVNLKFICDHYGWT
                     PNEVIRMRQDGRGFIDINRDIKDRKAGAKTQASTGSKDQPAKNKGKGKDKQK"
BASE COUNT         1700 a         2947 c         3415 g         1412 t
ORIGIN      
        1 ttgctgaggt ctattcgacc gacggcggtt ccctcgcgca gcgggtcctc gatgcccacg
       61 acccacggcg gcgagtcggg gtcggcgctg agggcccgga tgtcgccgcc gaagttggcc
      121 atgagggggg cccccgaggc gcggaacgcg aggtcggccg cccgatcggc cgcgtactct
      181 ttgccgagac cgccgagatc gatttccatg ccgggttgca ggcgcaggcg ccgcccatcc
      241 cattcgacct tctcccaccc gacccgtccg agcagcgact cgatcaggtc tcggtccggc
      301 gaagccgtcg atccgtcgaa tttccaggcc cggcgaagga cgccggaggt gatgtcgaag
      361 aggccgccgc tgaggcggta gcactggtcg gcataggtga ggaggcgcgc gagctcgtca
      421 tcgacttcca ccgccgcgcc ggtgccggcg ttgatggcgt ggattatgtt gtcgtcgcgg
      481 taccggctga gtttccgttc gatgcggcgc gtttccgcgg ccgcgagcga agccacgtgt
      541 tcggcttcgc tccggcggcg gcagcgaatg agaatctcgc agggactcgc catggcggag
      601 aaggaaatcg tccagtggga tccgcccttc cggacagagc actccggcgc ggccttagaa
      661 ttcatagtcg aatccggccc ggaccatgac ggcgtccatg tcggggaaca actcgtacgt
      721 gctcagcacg cccatcgaac ggggcgggct gatatccccc gcctggtgat agtactcgcc
      781 gccgaggctc acctgaaccc cctgaccgac cgggaacagg tactgcagcc cggccgtcac
      841 cgcgtgaaac ggagccagcc ggtagtcggc cgaggcatgg gccggcgccg gagcggcctc
      901 gaccaggaaa acgcggtaga agtccgcctc cgtctggcgg taccagcgga cgtggggctg
      961 gagcgcgtgc ccggctttca cgggcagtcg gtagaaaagt tcaacggtct gggaggtcac
     1021 gccccagtcg tcccagaagt agcggtagga gaggtcgatc gtgtgcccgc tcaggtagcg
     1081 gcgcagttgg gcgaaaaccg cctgcttggc gcggccgtcg ggacgggcct catagaggta
     1141 atcgaccggt tcgccggcgt cgggaccacc cggtccctgc acaacgctca ctatcttgta
     1201 gggatcgttc aggtagccgg acgagtggtt gtaggagtag ttgagacgga ggacggtctg
     1261 tcgattaagc acctgcgtga ggccgaagac gacgtcgtac acgttcttcc cctcgccggc
     1321 gtcgctctca cggtcctcgt cgtcatcgtc gtcgccgtcg tcatcctgag cggcgagctc
     1381 ccgctcgcct gcgtcgtcgt catcatcgtc gtcggcccgc gcgctcatcg aactcagcgg
     1441 gtcaggagga ccgccttcgg ggcggacccg gtcgcgcgaa taggcggccg aggcggacag
     1501 cgtcgtgttc ttccgattca ggtcgcggga gagaccgaca ctggccccca gggatgtgta
     1561 gtcatgctcg cccgagtagt gggcgccgag gacaatggtg gtgaggcggt cgagcggctg
     1621 agccagactg ccgtcgaagc tgtagcgcgt gtctttgaag gtgtcgtcga gcggaatctc
     1681 gcccggcttt acggaataac cgccctcgcc ggaggggcgg gcgaaggttt gcacgcgggc
     1741 cgacggcgtg gcgccgttgg gcgaggcgcc ggtgaggccg tcgagggtca gccgcgcgct
     1801 gagcatccgg tcccccttga gcgcccggcc caggccgatg atcgcttcgg ccgcctgcac
     1861 gcggtcgacc tcggaataca ggaggatcga cgattccacg cgattccgcc cctcggacgc
     1921 ggccgcgccg gtcccgagca gggccgcggt gagggcgctg agcgcgcctc ggagaggcgg
     1981 ccggttggtc aattgcatcc gcatcccccg ccgccgaagc tgcgcccgcc gctggaagcc
     2041 tccttgctga aatagatgtg gtcatccagg gcgcggtcga gcgcatggct gtccatctgc
     2101 atttccggtt tggagagaac gtcgcgttcc cagacctgca cgccgacggc ggcgcacccg
     2161 gagaggacgg ccgcgagcgc gaaagccccg gccgaaagca cgaagaatcg aagccgtctc
     2221 actgcgcccc tcccttgtcg agcaggcgga caatggccgc ctcgagactg tcggtctcat
     2281 ccgggcggaa gccccggtga gtggagacaa ggcggccgct ccggtcatag aggaaggagg
     2341 tgggcatcgc ctccagttca tagagcttgg ccagacggcc ggtcgaatcg aagacgatcg
     2401 ggaaagcggt gcggttctcc tcgaggaact tcaccgccgc gcggtggtcc tggtcgacgc
     2461 cgacggcgat gatctcgaga ccgcggttgc ggaacttgtc ggagagggcc tgcatccagg
     2521 ggaaagagtg gcggcagggc acgcaccacg aggcccagaa gtcgacatac acgaccttgc
     2581 cgttcaggtc gagcgaatcc gccagagcgg tctgcagtgc gttgagggag tcgggcgggg
     2641 cggccagaca ggggacggcg gcggccgaca gcaccagggc ggcgagcaac acgcccactc
     2701 cccgcgggcg gacgaaggcg atcatgaatc agcggctcct ttcccggcga gcgcgagcgc
     2761 tcggtccgtt gaggcggtaa ttcaggtttc gctatcgatc ggcggtacac gctccttcta
     2821 tcggaatggc ggggaagggg acatcccggt cccgctgtga tttctaaccg gggccgccgg
     2881 cgccggttcc aaaaaagatt tgacggctcc aaattggcca ttttttgcgc agcgatgaaa
     2941 agggttgccg ccgccggcgg tcaccggtcg gtgcgcgcgc tgtccccgac cagaggggcg
     3001 tcgtcccgaa tggtctcccc gaggcggccg ctcccccggc tgtccgagcc gggcggcggt
     3061 gtggccaccc ccccggccgt cttcgtgctc accgtcgtgt agccgtaggc ggtcgggaaa
     3121 tacagggcgg tgaggacatg gtcccaggtc tgggcgagcg ccggcgacgg ttgggtcgct
     3181 ttggcctcga ggcggaattc gtacgtgccg gcctctttgt agtacgtgcg cgtgaccacg
     3241 gcggggaagt agttggcgcc ggtgttgaca aagccgctca ggccggcctg cgtgtagtac
     3301 ggaaaaatcg ggagcccccc ctcgacggcg tcgatctgca cctgcgcgcc gttggggccg
     3361 gtggtgcccg atagcacggc gtagcacttg ccgtagagga cgatcaggcc gtcgtagggt
     3421 atggtgatct cgaccgtcac caggtcagtc atctcggcgg tcgagagcgt gatgatatcg
     3481 ctgctgatgc cgttgacgat gccgggctca tcgaggatct cgtcggcgtt gatcgccgag
     3541 tccgggaaca cgacggccga atcggaggcg cggccgccgc ggagcgcgat gccgagggcg
     3601 ccggcggtgt cgtagaggat gaaggcgccg cccgaatcat tgaccgtgcg gccgagcaag
     3661 ccgccggcag catcggtcag gtacaactgg cgcgcaacca tggccgacgg ggctgcggcg
     3721 agacgcgtgc ggggcgcgag gtccgctccc ccgagcgtga cctgcagaaa gagcgcactg
     3781 tgatcggcga acagggccga ggggaggtcg ctcacggccc cgagcaggtg cacgaacaga
     3841 ccgcggtagg tcggaacggt ggccgtctcc tgccacagcg gggcgccgcc cgtggagtcg
     3901 gcatagaggg agaaggtcat cgagtagagg ctgtccgcga tcgggagccc gtcgggatca
     3961 gtcagacggc cctgataaga gatcacttcg gagcgggccg agaaagccac cacgagcgcg
     4021 gccggcagaa tgagcgcggc ccaacgggat ccgaccacgt gcgacatcga gtctcctttc
     4081 cccacgcctg cccgcgtctt gcggaaacgg atcaggggac acggacaata tcggcatatt
     4141 cgggccgccg ggcaactcga tcctgcgcgg cgcaaatgct accgcccctc acctcaagag
     4201 cagcatcttg cggctcaaca ccgcgccgcc tgcctccaac cggtagagat agactcccga
     4261 accgaccggc cgtccgtcct tatcgagccc gtcccagatc accgcatggg tcccggcggg
     4321 gtacctttga tcggcgagca ccgcggtctc ccggccgagg atgttgtaga cagtcagggc
     4381 cacccggccg ggaagcggca ggtcaaagga gatgacagtc tgcgggttga acgggttcgg
     4441 gtagttctgc cggaggacgt aggcggaagg caggcgggga gaatccgggc ccgatgcgga
     4501 tgaccggaga gccggcaaag ggacacggct gccgtgaccc tgaaagacca ggggcgggag
     4561 aaccggagac ccgtcgaccc aaaccgtgag cgtgtccccc ggccgcgggt agaggggcgc
     4621 ggctgtggag gccccgtaga ccggggtgaa gggaaggatc ccgtcctcaa tcgccccccg
     4681 tccgcagagc acgccgcccg atgtgtggaa ttcaatgacg gcgccatcag caacaggcag
     4741 gccgtcgcag gcgggcccga cgccgtagac cgacatccag gtctgggtgg ccggtacggc
     4801 cggcgcggcc aggccgaagg gttcgtccgg cagaggccgg ccggcaggag aggaccatcc
     4861 cggatacacg aggtatccgt cccggtccgc gtggacccag tagccgaaac cgggggcgag
     4921 ctccgtgagc gtgttggcgc tgtcgccgtc gggaagccag atacggctgc ctccgtcaaa
     4981 gcccaggccg acgtcggtga attcgaggat ggaggcccag gcggtctcga cggggagcga
     5041 actgtcgggc cagcagggga ccaggttgaa cccgcggcgc accgggagcg tgtcgtgcgg
     5101 cgcgatcgct cctccgcaaa tatcgaacgt cgccgggcag gcgagccgga gccagtaggc
     5161 gcggtggtaa tcggcctcga agagggtcga gaattcgacc agattcggat cgtacgtgag
     5221 accgccccgc tcaaaaccca ggaccacgtc gacacaggcg gcgccgccga ggagcgagac
     5281 gagggagtcg atgtcggcgc ggaaggcgcg gttccaggag atgaggtgcc agcccgggtc
     5341 aagcgcgaag tgccggcagg agacgcgatc gcagacttcg atagtgtcgc ccggcgccct
     5401 gaaaaaaacc ggcggcgtca gcaggacctc aacagcgttc agggtgaagg tcagcaggtc
     5461 gcccggttcc gcgccctcgt caacgagcgg agtggagggg tcgtcgccga ccacgcgcag
     5521 cgagccgaag gtggaatcat cgccgaccac ggcgctcccc gagaggacgc cgtcgggatc
     5581 gaaaaccctt agggagtcgc ccggccgcag cgatcggccg gcgaaaaccg ggccggaaca
     5641 gacaagcgca atccagttgt cctcgggcag caggccgcag tgccaatcca gcggcacggt
     5701 tgtgcgggag aggcccagat tatcccatgc ccggacagac aggaggaggc gggcgttttg
     5761 cggcacgcgc ggaagggcaa ccgcaaacgt atcgcccccg accgggagca tgtcgtacac
     5821 actcggaagg ccgccgtcga acacgacggc gatggcacgg gcgacaccgt cgggatcgtc
     5881 gcgcacgacg gcagagagga gcgcacccga gtcgacacac accgcggccg ccgcagaaat
     5941 ctcgggcggc tggttaggac agaaactgac cgcgaaggga agctggaagg gatcgacggc
     6001 gggcagggtg acggtgacgt cgccgttggc cgcgctgatg aaatagtcga agcccgccgc
     6061 gtccggcggc cacgcgccgg ccgggaggac caccgcatac cggtccccgc cgaccgcgat
     6121 catggcgaca tcccggtacg gctccggagc ggctcccacc tggcggtaga agagccgtac
     6181 atcctgaagc cctgcgccgc cgggatcctc tgcgagcgct tcaatcgtga tatcttccgc
     6241 ggccggttga caggtgtcgc tgagctgcag ggtcgtgggg gcgaggtgga ttgtcgggac
     6301 cgccggttcg cgatagaccg cggtgtcgca gcacacggcg ccaccgagcg tcgcgcacac
     6361 ttcgacaaga tggaaatcgt ggtcggcggc ggagtcgggg ctgaagtagg tgatcaggta
     6421 gcggctgcac acggcgtgct ggatggcggc gaaaacggag tcgacggcgt cggcatccga
     6481 tgcgtaccga tgcagaccgc cggagccgcg ggcgaacgcc tccattgcat cgccgcagac
     6541 ggagtcgagg gtgccgaagg tgagagtgta gacggagacg gcggcggcgc gggcgagatc
     6601 gcagacaagc gccgaatcgt cctcgtagcc gtcggcccaa ccgtcggccg gaagatcgca
     6661 gtaggcgctg ttgttgtcac agccgtcgga gaaagcaatg acagcacggt tgcccggctg
     6721 ggcggcggcc agttggacgc ccaggtacac gccgtcgaac gaggcggtca aaccgagagc
     6781 aacgagcgag tcgacggcgg cgtgaagagc ggcgcggtcg tcggtgaacg gagcattgac
     6841 catggcgcag tcggcaaagg agaccacggc ggcgcggtcg aacggcccca tccggtcaac
     6901 aaaccggtgg gcggcccggc gggctgaggc gagcggctcg cccgccatgc tcaggctgac
     6961 gtcaaaaacc aggcagaatg ccgccgggca gccaatctcg gccgcgggaa gcagggaaaa
     7021 agagaccggc gcaccgtcct gggtcaccgc aaagtcgggc cccgcgagac ccgcaaccgg
     7081 cccgtcgctc tcatccagga cgtcggcgta gacgcggatg agcgggaagc ctgaccgctc
     7141 cacggtgatc gacagagacg tgtcgccggc gctatccccc gactgaagcg ggcgggatcg
     7201 aaggagtgtc gggtcaaaag gatcgccggc cgggccgatc aggccggagg cggccccgag
     7261 caggccggcc agcagcacaa cgcccagccc ggctgcaact attctgcgca tcttctccac
     7321 actctccttg tgtcgtcaat tcgataatgg cggcgagttt cgtccagcca gtacagggtg
     7381 accgcgcggg gtgatcgcag aggctgtccc cacgctgctg ccgaacgcaa gatacgggcc
     7441 gtcgtcgggc gaagtcaacg gttatcgccg gagcgcggct tccagggcgt cgtccagccg
     7501 cggggcgaga acaggccaga agaagcgctc ggccgcggac gccccggcgg ggagggacgg
     7561 cgaccggagg gagccggtct cggcgaagcg tcgggcgagc atgacaagtt tgtcgaccag
     7621 cccggccacc gtaccgtcat agtaccagtc gggaaacgat tgcgggggga atatctcggg
     7681 gtaggcgagg cgttgaggga gcacgggccg gatcccggcg gcgaccgcct cgacaatggc
     7741 cacgccgaag aattcatggc gggcggttga tacgatgagg tcggcggcgg ataaggcgcg
     7801 ctcgtagtcc gcgcggtcgg gctgataacc ccagcggtcg atgagcgggg cgaattcccg
     7861 ccgggcctcg gcgaaggccg ccggtacctc ggaaaactgc tcgccgatca cgctgagacg
     7921 aaaatcgacc ccccgggagc ggagcgcgcg catggcggcg aagaagtcct ccgggttctt
     7981 gtcatgctcc caccgggcgg cccagaggat ccgcagcggt ccgggcggcc gggctgaaag
     8041 cggccgcggc ggggcgatcc cctggggata cacctccgaa cgcgcggcga tccggtcgac
     8101 ccaggccagg ggagggtagt cgggcatccg ccggaaaaag gaccgcaacc cctcgagaaa
     8161 ctcgcggcgg tggaaatccg agttgaacca gacgcccgcg gcgaccgctg ccgtcgaaaa
     8221 gttcgtcatg gcgaagtggt agtcccgctc cccggcatgg agactggggt aggtgagttg
     8281 gttctcgtgg aagtagacaa cggccgggag gttgccgaga gcggcgcggg ccagccccag
     8341 cagttgggcg aggtcgacca tgtcggtggc gaagagaccg tcccaccgct cgccgtctcg
     8401 ccggcgggcc gcaatttcat cgcagaaggt caagggcgcg tggcgcatcc gccacttcca
     8461 cttgtagccc ggcagggtga gcaaggtcca gcggtggcgg ctgccggcaa tccaaccgtc
     8521 cacgaaggcg cggtggctgc cggcgtagta tggttccagg gcgagcagat gcatcaccga
     8581 tctccggctg caaggaacgc cgccgcccgg cccttgtcaa tcgggctccc ccaaaaaagc
     8641 cacgggccgc cggagaggcg gcccgtgtcg gggatggcgg tgttgaggga tggattactt
     8701 ctgcttgtct ttccccttgc ccttgttctt ggcgggctgg tctttcgatc cggtgctggc
     8761 ctgtgttttc gcgcccgcct tccggtcctt gatgtcgcgg ttgatgtcga tgaaaccgcg
     8821 gccgtcctgg cgcatcctga tgacctcgtt cggggtccag ccgtagtggt cgcagatgaa
     8881 tttgaggttg acgaagttca cgacatcgtc gtcgctcagg cggatttcac cccaccggtt
     8941 tttcggatgc ttcttgaagt ggccgtaggc gtggccatag ggcgggcccg acacgtcctc
     9001 gaccggcaca tagaatatct cggcggtctt gccgaactgc agggtgatgt ccatccagga
     9061 cttgccgccg aggcgcaggt cgatgatggt cgccggggag gcgttgcagc gggcggccag
     9121 gaagaagacg accggcatct cttcatcggg aatgtgccgc tcgcggacca ccacaatctc
     9181 tttctccgga acctggtagt ggttgctgac tgccaggtag aaactcttgc gcccttcgtc
     9241 atccacctgg aggccgaagc cgaagtcgac ggctggggcc agggtggcgg tcgcaagaaa
     9301 cagggcgatc agcagggggg cgtttttgcg catatcatcc tccagggaag gtccggtgta
     9361 cctgataggt atatcggctg ggcgggcgcg gtaattgagc agcgaattcg accgcccggc
     9421 caaggattag cgcttgccag ttttggtgtc atgagccaga ttgctggaaa cctg
//
LOCUS       DAOPOE010000108         9467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8191_length_9467_cov_4.794199, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000108 DAOPOE010000000
VERSION     DAOPOE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_9467_cov_4.794199"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKM94_11770"
     CDS             <1..558
                     /locus_tag="PKM94_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67353.1"
                     /translation="AVGFALVAFVQLPFQDYLFEALYRPRVAAGSTALPWALLVVLPA
                     GLIQEGLKLLGVVAVGQWRRIPAHYGMATGAAVAAGLGIIEACYLDTVIIDPQLFTWP
                     FLQRVLIILFHATTGALIGQAYFASKRLLWGVLGLAVALNMILRAMPILVQARLMDAG
                     LVNITTGFLVVLLMLGAVVWQRRAA"
     gene            complement(637..822)
                     /locus_tag="PKM94_11775"
     CDS             complement(637..822)
                     /locus_tag="PKM94_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67354.1"
                     /translation="MDKLESNGVTAYIDPGLNNFIRNYGDIHIDYINEGPGRQGFMLR
                     VGSANCGESSGGCSSCG"
     gene            1237..2508
                     /locus_tag="PKM94_11780"
     CDS             1237..2508
                     /locus_tag="PKM94_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOD67355.1"
                     /translation="MIEHICIFEDGHFRDFYPLTLTRPVCLMRAGIFPQYERIRRRLE
                     AQAVSLLCRDQIAPLVAEQTPRHPVNIIKRGDSDLLFVNGGIRDFGDLPTMLADDRHT
                     RAFESEGRVAAVLLKADTLRALPPVAAHDDYIRFLLAENENIPRHTTTATLYRGAWEF
                     VEDIETAVTADFRALEPEFSAPQQVRVHDGAYFVNENDVFLGDGVRVLPGAVIDASKG
                     PVYIGANSAVEPHAAVAGPCYIGPNSTVLAGKITASSIGHTCRVGGEVEESVFQAYVN
                     KYHAGFIGHSYVGQWVNFGALTTNSDLKNNYSTIRVTVNGTSVDTGAIKVGSFIGDHT
                     KFGIGTLLTTGIAVGVCCNIFGGTLVADKEVASFRWGSGGAWSPHRFDKALETARRTT
                     ERRHVSLSDREAALLQAIADETLGDAGVLPL"
     gene            2575..3621
                     /gene="galT"
                     /locus_tag="PKM94_11785"
     CDS             2575..3621
                     /gene="galT"
                     /locus_tag="PKM94_11785"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOD67356.1"
                     /translation="MPELRKDPVIGRWVIISTERGKRPSSFQSVSKRGPARLCPFCPG
                     AESNTPREILAYRDPGTEPNKPGWSLRIFPNKYPALVIEGNLTREPHGVYDKMQGIGA
                     HEVIVETADHARDMDGMSEKELRDVFWAYRERMIDLERDRRFRYILVFKNHGQAAGAS
                     LEHSHSQLIATPIVPKKVQEEIEGSRRYYEFKERCVFCDIVRQEIMDQERIVTDFDSF
                     ITMQPFAARFPFETWLLPKTHQSSYLEMSDSDFWILARTFKDILTRLKVALNDPPFNF
                     VLHTRPMSPEHHTYYHWHFEIIPKLTRVAGFEWGSGFYINPTTPEQAAAYLRQIDVTA
                     PPAAAPSEISEKEV"
     gene            3624..5084
                     /gene="glgA"
                     /locus_tag="PKM94_11790"
     CDS             3624..5084
                     /gene="glgA"
                     /locus_tag="PKM94_11790"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOD67357.1"
                     /translation="MKVCLVGPEMVPFAKTGGLGDVVGALPKALGRLGHDVTVFMPLY
                     RELDFGVHRVHAVDLAVSVPVAGSVQTVSLRAVRQKKHNVEIYFIGNRQYFDRDGFYV
                     DPATGKDHADNDERFIFFNRAVLEVLKALNLRPHVINVNDWQTALIPVYLRTLGAADP
                     FFAGVKTVLTIHNLAYQGSFPRERFARLELPGALFAPAAGPFEFYGQVNFLKAGITFA
                     DKITTVSKQYAREIQTAEFGAGLEGVLAGRAADLVGIQNGVDYTVWSPSRDKKIHYTY
                     HLANLSGKRMNKVELLNRAGLPIRDSAPLIGIISRLVDQKGFDLIAEAADRIFAMNLQ
                     MIILGTGETRYHDFLTRLQAAYRDKVRAYLMFDDTLAHQIEAAADIFLMPSRFEPSGL
                     NQLYSLKYGTVPIVRRVGGLADTVTDYDPATGEGTGFVFDDYTAEALTGAIARAVLLF
                     ERKRVWTKVMKRGMAEDYSWQHTAEEYARLYRDLVS"
     gene            5194..7080
                     /locus_tag="PKM94_11795"
     CDS             5194..7080
                     /locus_tag="PKM94_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67358.1"
                     /translation="MPARARERAEALLWLIPALALYALFVLRLEFVQDDAFISFRYVA
                     NFLNGHGLVYNIGERVEGYTSPGWVVLLSLAGALGFDYPAVARFLGILCGAGLPVLAW
                     AVARRTEPQRGPLAAAIPAYLVAATASLAYWAAAGLETTAFALAAGVSLYGFLVRRVP
                     LLAVGLVWAVWLRPEGAVVAAILIAVETLVIKRFPRMTVAAGALAFVLSLPMVAFKLG
                     YYGALPPNSFYAKTGWDFEQIRGGAEYAGQFFTHYPVAAFGLLAGLVTWRASGPTVRA
                     IGLFSLFYVVYVILIGGDVLKVHRFFLPILGPLMVAAVYGAAGAVEMVARRRRRWTGA
                     AVAAALAAMVLTWLLPRAYIENYRHRERMFVARMTAMAEQMKATDPTDFSVALSTIGA
                     FGYTLTGHRVIDMLGLTDSAVARHPEPIPEGIESTWRERSFNCRYLLSQGPDYILFST
                     NNKPSAPAELVLTLYPQFQRSYRAVGWHVPGSGLQVAFRRQEPITGALKPAAPAAFAL
                     AYKRGLEQQWKGDLVGALNSFDQALAVSPPPYYLDLVFRKALCHRDLGQMDEALRLLQ
                     WIVAQDPRMVDAQRELYMIETLRGNPAQAAEHRAWVEKMTPWEMPGLDSAVAEVARSA
                     RRTP"
     gene            7250..7322
                     /locus_tag="PKM94_11800"
     tRNA            7250..7322
                     /locus_tag="PKM94_11800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7283..7285,aa:Gly,seq:gcc)
     gene            7333..7404
                     /locus_tag="PKM94_11805"
     tRNA            7333..7404
                     /locus_tag="PKM94_11805"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7365..7367,aa:Cys,seq:gca)
     gene            7637..7873
                     /locus_tag="PKM94_11810"
     CDS             7637..7873
                     /locus_tag="PKM94_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67359.1"
                     /translation="MNQAVAEKLVLAGRMYRYRRPPRVVALELPASLTLNFGAWGRHG
                     KAGDFLVKNLQSNELDILPRETFLEMCEPVQRRR"
     gene            complement(7884..8186)
                     /locus_tag="PKM94_11815"
     CDS             complement(7884..8186)
                     /locus_tag="PKM94_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67360.1"
                     /translation="MILRCPQCHSIHDFTPAASYIKQMLRDGHLIVRCRRCGLGLVPM
                     PQVMPPAGRRRRRRRLRDINVPINRLSLLYELMDPSFGDFITKAWGNHPRPFTTHQ"
     gene            complement(8183..8461)
                     /locus_tag="PKM94_11820"
     CDS             complement(8183..8461)
                     /locus_tag="PKM94_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67361.1"
                     /translation="MLKAILRRPIVALYHWVYRDTVIVRGNASYVNGRLFTSDDFHTG
                     AFRTFAVEFDEPCPVSAFVEAIAEHPDTPGGGMRLACNNTRLISDLEP"
     gene            8787..8969
                     /locus_tag="PKM94_11825"
     CDS             8787..8969
                     /locus_tag="PKM94_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD67362.1"
                     /translation="MKITDDCTACGLCLPECPTQSISEGDIYVIDPGSCNECADQKDG
                     PQCAAICPVEDCIVKA"
     gene            complement(9203..>9467)
                     /locus_tag="PKM94_11830"
     CDS             complement(9203..>9467)
                     /locus_tag="PKM94_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67363.1"
                     /translation="PPQKSRKVTIPNRASEWIEVAAVYQLDQHFSVNVVTPKAQKGGA
                     IELEVVPSPELARGTLESNFTVTVTSADSDPTVLTVPVKIVKY"
BASE COUNT         1679 a         2975 c         3045 g         1768 t
ORIGIN      
        1 gcggtcggct tcgccctggt cgcgtttgtg cagctgccgt tccaggacta tctctttgaa
       61 gcgctctacc ggccgcgggt ggcggccggg tcgaccgcgc tgccttgggc gctcctcgtc
      121 gtccttccgg ccggactgat tcaggagggg ctgaagctgc tgggcgtggt cgcggtgggg
      181 caatggcggc gaatcccggc tcattacggc atggcgacag gagcggctgt tgccgccgga
      241 ctggggatta tcgaagcctg ctatctggac acggtcatca tcgatcccca gttgttcacg
      301 tggccgttcc tccagcgcgt cctcatcata ttgttccacg ccacgaccgg cgccctcatc
      361 gggcaggcct acttcgcgtc gaagcggctc ctgtggggcg tgctcggcct ggccgtagcg
      421 ctcaacatga tcctccgggc catgccgatc ctggtccagg cgcgcctgat ggatgccggg
      481 ctggtcaaca tcaccaccgg attcctcgtc gtgctcctca tgctgggggc agtcgtgtgg
      541 cagcggcgcg ccgcctgaca tcgccggaga gagagaaaaa aggcgggttg cccgaaagcc
      601 gcccgcctcg tgatttctgt ctgaagatcg gccgtgtcag ccgcaggaag aacatccccc
      661 cgaactctca ccgcagttgg cgctgccgac gcggagcata aacccctgcc gtcccggccc
      721 ctcgttgatg tagtctatgt gaatgtctcc gtaattcctg ataaagttgt tcaggccggg
      781 atcaatatag gcggtcacgc cgttcgattc gagcttatcc aatccatccg ttgactcatc
      841 cagagccagt cccaaattgg gaccgcttca gccgacgccg gcgaaggtga cgatgaggct
      901 tttgcctttg gcatggtcgg aatcaagaac cttgcgcagt tccgttccgg cggtatctga
      961 gacctcgaac atgctgtacg tcctcacttt cgttgtcgca atcacctgtt agatcaaaat
     1021 caatctacaa tgattcacac aaatagccac atcaaataac atccaccgcg cggtccggtt
     1081 cccgcagcgg taatgtcgat ttcccggtat aagttactga gatagcaaaa cttcacaaat
     1141 ttcgcttgat tcctgtcccc ccgcctccca tctttgccgg agtgactgcc ctgcccgatg
     1201 gggccgggcc ctgcggaacc gtgaaaggca caggcaatga tagaacatat ctgcattttt
     1261 gaggacgggc attttcggga cttctatccg ctcacgctga cccgtccggt ctgcctcatg
     1321 cgggccggca tcttcccgca gtacgagcgg atccgccgcc ggctggaggc tcaggccgtg
     1381 tcgctgctgt gccgcgacca gattgccccg ctggtggccg aacagacgcc cagacatccc
     1441 gtgaacatca tcaagcgcgg cgacagcgac ctgctgttcg tcaacggtgg catccgcgat
     1501 ttcggcgacc tgccgaccat gctggccgat gaccgccata cccgcgcgtt tgaatcggag
     1561 ggccgggtgg cggccgtgct cctgaaagcg gatacgctca gggcgctccc gccggtggcc
     1621 gcccacgacg actacatccg cttcctgctc gccgagaacg agaacatccc gcgccacacg
     1681 accactgcga ccctctaccg cggcgcgtgg gagttcgtcg aggacatcga gacggccgtg
     1741 acggccgact tccgcgcgct cgaacccgaa ttcagcgcgc cgcagcaggt gcgagtccac
     1801 gacggcgcct acttcgtcaa cgagaacgac gtttttctcg gcgatggcgt gcgggtgctg
     1861 ccgggggcgg tgatcgacgc ctcgaagggg ccggtatata tcggcgccaa cagcgccgtg
     1921 gagccccacg ccgcggtcgc cgggccatgc tatatcggac cgaactcgac cgtgctggcc
     1981 ggaaaaatca ccgcctcctc gatcggccac acctgccggg tcgggggcga ggtggaggag
     2041 tcggttttcc aggcctatgt gaacaagtac cacgccggtt ttatcggcca cagctatgtc
     2101 gggcagtggg tgaatttcgg ggcgctgacc accaactcgg acctgaagaa caactactcg
     2161 accatccgcg tcacggtcaa cggaacctcg gtcgataccg gcgcgatcaa ggtggggtcg
     2221 ttcatcggcg accacacgaa attcgggatc gggacgcttt tgacgacggg aattgcagtc
     2281 ggagtgtgct gcaatatctt cggcgggacc ctggttgccg ataaagaggt agcgtcgttc
     2341 cgctggggat ccggcggggc gtggtcgccc caccggttcg acaaagcgct ggagacggcg
     2401 cggcggacga cggagcggcg ccacgtcagc ctctccgacc gggaggcggc gctgctgcag
     2461 gccattgccg acgagacgct gggcgatgcc ggcgtgctgc cgctgtgagg acgagacggc
     2521 cgaacggaca gaggggccga aggtaacgga agaccacaaa gggagtgatg ccgtatgccc
     2581 gaattgcgta aagacccggt gatcggtcgc tgggtgatca tttcgaccga gcgggggaaa
     2641 cgcccgagtt cgttccagtc ggtctccaaa cggggaccgg cgcggctgtg cccgttctgc
     2701 cccggggcgg agagcaacac cccgcgtgag attcttgcct accgcgatcc gggcaccgag
     2761 ccgaacaagc ccggctggtc gctccgcatt ttccccaaca agtaccccgc cctcgtcatc
     2821 gagggaaacc tgacccgcga gccgcacggg gtctacgaca agatgcaggg gatcggcgcc
     2881 cacgaggtga tcgtcgagac ggccgaccac gcccgcgaca tggacgggat gagcgagaag
     2941 gaactccgcg acgtgttctg ggcctaccgg gagcgcatga tcgacctcga gcgcgaccgc
     3001 cgtttccgct acatcctcgt gtttaagaac cacggccagg ccgccggcgc ctcgctcgag
     3061 cactcccaca gccagctcat cgccaccccg attgtcccca agaaagtcca ggaggagatc
     3121 gaggggagcc gccgctacta cgaattcaag gagcgctgcg tgttctgcga catcgtccgc
     3181 caggaaatca tggaccagga acgcatcgtg accgacttcg actcgttcat cacgatgcag
     3241 ccgttcgcgg cccgcttccc ctttgaaacc tggctgctgc cgaagacgca ccagtcgagt
     3301 tacctggaga tgagcgattc cgacttctgg attctggccc ggacattcaa ggatattctc
     3361 acccgtttga aagtggcgct caacgacccg ccgttcaatt tcgtgctcca cacgcggccg
     3421 atgtcgccgg agcaccacac ctactaccac tggcatttcg agattatccc caagctgacc
     3481 cgggtggccg ggttcgagtg gggctcgggg ttctacatca atccgaccac ccccgagcag
     3541 gcggccgcct acctccgcca gatcgacgtc accgcgccgc ccgcggcggc gccgtccgaa
     3601 atcagcgaga aggaagtata gacatgaagg tctgtttggt gggaccggag atggttccgt
     3661 tcgcgaagac gggaggactt ggcgacgtgg tgggggcgct gcccaaagcc ctcgggcgcc
     3721 tcggccacga cgtcaccgtc ttcatgccgc tctaccgcga gctcgacttc ggcgtccacc
     3781 gcgtccacgc ggtcgacctc gctgtgtccg ttcccgtggc cgggtccgtg caaaccgtct
     3841 cgctccgcgc cgtgcgccag aaaaagcaca acgtcgaaat ctacttcatc ggcaaccgcc
     3901 agtatttcga ccgcgacggg ttctacgtcg atcccgcgac cggcaaggac cacgccgaca
     3961 acgacgaacg cttcatcttc ttcaatcgcg ccgtgctcga agtgctcaaa gcgctcaacc
     4021 tccggcccca cgtgatcaac gtcaatgact ggcagacggc actcatcccg gtctacctga
     4081 ggaccctcgg cgcggccgac ccgtttttcg ccggcgtcaa gaccgtcctc accattcaca
     4141 acctcgccta ccaggggagc ttcccccggg agcggtttgc caggctggag ctgcccgggg
     4201 cgctcttcgc cccggcggcg gggccgttcg aattctacgg ccaggtcaat ttcctcaaag
     4261 ccggtatcac cttcgccgac aagatcacga ccgtctcgaa gcagtacgcc cgggagatcc
     4321 agaccgccga attcggcgcc ggtctcgaag gggtgctggc cgggcgggcc gccgacctcg
     4381 tgggcattca aaacggcgtc gactacaccg tctggtcccc ctcgcgcgac aagaaaatcc
     4441 actacaccta tcacctggcc aacctctccg gcaagcggat gaacaaggtc gagctgctca
     4501 accgggcggg gttgccgatc cgcgacagcg cgccgctcat cggcatcatc tcgcgcctgg
     4561 tcgaccagaa gggattcgat ctcatcgccg aggcggccga ccggatcttc gccatgaacc
     4621 tgcagatgat tatcctgggg acgggtgaaa cccgctacca cgattttctc acccgtttgc
     4681 aggcggccta ccgcgacaag gtccgggcgt acctcatgtt cgacgacacc ctcgcccacc
     4741 agatcgaggc cgcggccgac atcttcctca tgccctcgcg gttcgagccg agcggcctga
     4801 accagttgta ttcgctcaag tacggaacgg tcccgatcgt gcggcgggtg ggcggcctgg
     4861 ccgatacggt gaccgattac gacccggcga ccggcgaggg gaccggcttt gtgttcgacg
     4921 actacaccgc cgaggcgctc acgggggcca tcgcccgcgc cgtgttgctg ttcgaacgca
     4981 agcgcgtctg gaccaaagtg atgaaacgcg gcatggccga ggattactcg tggcaacaca
     5041 cggccgagga gtacgcccgg ctctatcgag acctggtctc gtgagaccgg tccggcgcaa
     5101 gaagtaattg ccgcgacgac ccgggccgca ccgggccgtc tttcacgacc cgcccgagca
     5161 caatctcggg gcgggagcgg agatgacagc accatgcccg cgcgagcgcg cgaacgtgcg
     5221 gaagcgctcc tttggctcat tccggccctg gcgctctacg cacttttcgt ccttcgattg
     5281 gaattcgttc aggacgacgc cttcatttcc tttcgctacg tcgccaactt cctgaacgga
     5341 cacggtctcg tgtacaatat cggggagcgg gtcgaggggt acacgagccc cggctgggtg
     5401 gtgctcctgt cgctggccgg cgccctcgga ttcgactacc ccgcggtcgc ccgcttcctc
     5461 ggcatcctct gcggcgccgg gctcccggtt ctcgcatggg cggtggcgcg tcggacagag
     5521 ccgcagcggg gcccgcttgc ggccgcgatc ccggcctatc tggtggcggc cacggcctcg
     5581 ctggcctact gggccgcggc cggtttggaa accacggctt tcgcgctggc cgccggcgtg
     5641 agcctgtatg ggttcctggt gcgccgcgtg ccgctgctgg cggtcggtct ggtgtgggcg
     5701 gtctggctgc gcccggaggg cgccgtggtc gccgccatcc tcatcgccgt cgaaaccctc
     5761 gtcatcaagc gctttccccg catgaccgtg gccgccggcg ctctcgcttt cgtcctttct
     5821 ctgccgatgg tcgcgtttaa attggggtac tacggcgccc ttccgccgaa ttcgttctac
     5881 gccaagaccg gctgggattt cgagcagatc cgcgggggag cggagtacgc cgggcagttt
     5941 ttcactcact acccggtggc ggccttcgga ctgctagccg ggctggtcac gtggcgggcc
     6001 tccgggccga ccgtccgcgc gatcgggctg ttctcgctgt tctatgtcgt gtatgtgatc
     6061 ctgatcgggg gggatgtgct gaaagtccac cgcttcttcc tgccgattct ggggcccctg
     6121 atggtggcag ccgtgtacgg cgccgcgggg gcggtcgaaa tggtcgcccg gcgaagacgg
     6181 cggtggaccg gggcggccgt agcggcggcg ctcgcggcca tggtgctgac ctggcttttg
     6241 ccgcgcgcct acatcgagaa ctaccgccac cgcgagcgga tgtttgtggc ccgcatgacg
     6301 gccatggccg aacaaatgaa ggccaccgac ccgaccgatt tctcggttgc tctgtcgacg
     6361 atcggcgcgt tcggctacac cttgaccggc caccgggtga tcgatatgct ggggttgacc
     6421 gattccgccg tggcccgtca ccccgagccg atacccgagg ggatcgagtc gacctggcgg
     6481 gagcgcagtt ttaactgccg ctacctgcta tcgcaggggc cggactacat tctcttctcg
     6541 accaacaaca aaccctcggc gccggccgag ctcgtgctca cgctgtaccc gcagttccag
     6601 cgctcctacc gcgcggttgg ttggcatgtg ccggggagcg ggctgcaagt ggctttccgc
     6661 cgccaggagc cgattaccgg agcgctcaag ccggccgctc cggcggcctt tgcgctcgcc
     6721 tacaagcgcg gtctggagca gcagtggaaa ggggatctcg tcggggctct gaactctttc
     6781 gatcaggcgc tggcggtttc gccgccgccg tattatcttg atttggtgtt ccgcaaagcc
     6841 ctttgccatc gcgacctcgg gcagatggat gaggccctgc ggctgctcca gtggattgtc
     6901 gcccaggacc cgcgcatggt cgatgcccag cgggagctct atatgattga aacgctgcgc
     6961 ggcaacccgg cgcaggcggc ggagcaccgg gcgtgggtgg agaaaatgac cccttgggag
     7021 atgccggggc tggactcggc ggtggccgaa gtggcccgct cagcccggcg gacgccctga
     7081 gggtggtttg tcggaaacag cttgtgtgtg cgccggccgg tccggtgaag gaagggatcc
     7141 cgggaataat cgtgggggaa ccccccgggc cggttcggca cacaaattcg ccgtcaaagg
     7201 tattgacaag ctttcggggc gaagttaata ttagatgctt tgtgagaaag cgggaatagc
     7261 tcagttggta gagcaccacc ttgccaaggt ggctgtcgcg agttcgagtc tcgtttcccg
     7321 ctttttttgt cgggcggcat agccaagtgg taaggcggag gtctgcaaaa cctctattcc
     7381 ccggttcaaa tccgggtgcc gccttttttt cgtatcattt tcaagcacat agcgttagaa
     7441 acaagctgtc agcctcttga gataagcctg agggctataa cttgagaaga ctgcgcaaga
     7501 atggcgcagg ctgcgccatc ggctgcgcca tcgtgaatgg cgtacctgcg ccatcggccg
     7561 cggcgcacaa caggtgtaaa aacctgtgca attttcgtgt aatcgtgtag ttttcgcctg
     7621 atccagaatt tcagtggtga accaggcggt ggccgagaaa ctcgtgctgg cgggaaggat
     7681 gtaccgctac cgtcgcccgc cccgggtggt ggcgctcgag ctgcccgcca gtctgacgct
     7741 gaacttcggc gcctggggac ggcacggcaa ggccggggac ttcctggtga agaacctcca
     7801 gagcaacgag ctggacatcc tcccgcggga gacgttcttg gaaatgtgcg aaccggtgca
     7861 acgccggaga tagaaacgga tgtctattga tgcgttgtga acggcctggg gtgattgccc
     7921 caggcctttg ttatgaagtc cccgaaggag gggtccatga gttcatagag cagggagagg
     7981 cggttgatgg ggacattgat gtcgcggagg cggcgccgcc ggcgtcggcg gccagccggg
     8041 ggcatgacct ggggcatggg caccagcccc aagccgcagc gccggcagcg cacgatgagg
     8101 tggccgtcgc ggagcatctg cttgatgtaa ctggccgcgg gcgtgaagtc gtggatggag
     8161 tggcattggg ggcagcgcag gatcatggct ccaggtccga gatgagacgg gtgttgttgc
     8221 aggccaggcg catccccccg cctggggtat caggatgttc ggcgatggct tcgacgaaag
     8281 cggaaaccgg gcagggctcg tcaaactcca cggcgaaggt gcggaaagcc ccggtgtgga
     8341 agtcgtcgga ggtaaagagc cgtccgttga cgtagctggc gttgccgcgg acgatgacgg
     8401 tgtcccggta aacccagtgg tagagggcga cgatgggtcg ccgcaggata gctttcagca
     8461 ttttactcag tctcccggtg gtgtgcgtgg gggcctgacg cctgtaatat acaccgatcg
     8521 acgaccgttg gcaagcaggg atcggtgagg aaggcgcgca ggtaggcccg ttcccccatg
     8581 gcggccgtcc gccgccggcc acctgctgag gggcgcgtgt cgggtgactc atgtggccgc
     8641 cccggccgtg ggggcggcct tcccgcaagt gacgagccgg cgcgttttcc gcatggcgcg
     8701 gccgacgggc gcgctgtata atgccgcgtg tgcgattact gccggcggcg cccgcggccg
     8761 ccaagattgg agaattgccc gtggctatga aaatcaccga tgactgcacc gcctgcgggc
     8821 tgtgcctgcc ggaatgcccc acccagtcca tttcggaagg agatatctac gtcatcgatc
     8881 ccggctcctg caacgaatgc gcagatcaga aagacggtcc gcagtgcgcg gccatctgtc
     8941 cggtcgagga ctgcattgtg aaggcgtagc tcgggacaga gccggagaaa cagccagggc
     9001 ggcgggggag aggcggtcgc cggcgggcct acgtcagttc ttggggtggc tccgtcaaac
     9061 ccgccggtcc aaaaaataga gggtgctcct ctgtttgggt agagggggct agaaaactac
     9121 ccagggacag cagagaatca ccctcgttgt cctataaatg acacaatccg tcagggatgt
     9181 caaggattaa tacggaagat tctcagtact ttacgatctt cacggggacg gtcaggactg
     9241 tcgggtcgct atcggccgaa gtgacggtca cggtaaaatt cgattccagc gttccgcggg
     9301 caagttccgg tgacggaaca acttccagct caattgcgcc ccccttctgg gctttcggcg
     9361 tcactacgtt aacggagaag tgctggtcca actggtagac cgcggccact tcgatccatt
     9421 cggaagcgcg gttcggaatc gtgactttcc tggatttctg agggggc
//
LOCUS       DAOPOE010000109         9338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8339_length_9338_cov_5.081870, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000109 DAOPOE010000000
VERSION     DAOPOE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8339_length_9338_cov_5.081870"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..446
                     /locus_tag="PKM94_11835"
     CDS             135..446
                     /locus_tag="PKM94_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOD67364.1"
                     /translation="MLFQVSMFPTSTGKSSVSAEVARVIDLIDRSGLPYKLGAMATVI
                     EGEWEPVMALLNRARRMLRRRGHERVYMVITIDDRKGAKNRLAGKVLSVEEKLGREVM
                     K"
     gene            443..769
                     /gene="cutA"
                     /locus_tag="PKM94_11840"
     CDS             443..769
                     /gene="cutA"
                     /locus_tag="PKM94_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOD67365.1"
                     /translation="MNSEVCVVFVSLPRDEARNMAKALVENCLAACVNILPRIESYFT
                     WEGRAEFDEEALLVIKTTPARFEALKGYVEENHPYELPEIIAVPVTQGLSEYVAWVKK
                     ESVSEA"
     gene            812..1261
                     /gene="nrdR"
                     /locus_tag="PKM94_11845"
     CDS             812..1261
                     /gene="nrdR"
                     /locus_tag="PKM94_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOD67366.1"
                     /translation="MKCPNCGHEEDKVVDSRKSHDGRAVRRRRECLNCKARFTTYEYV
                     EHQQITVLKSDGRREPFDRNKIEYGIKLACNKLPVSREQIKAMVDEIEAEVNARVGNE
                     IRSADIGELVMERLRAVNDIAYVRFASVYRQFRDTAEFLEEIKKLLK"
     gene            1530..2360
                     /gene="tatC"
                     /locus_tag="PKM94_11850"
     CDS             1530..2360
                     /gene="tatC"
                     /locus_tag="PKM94_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOD67367.1"
                     /translation="MADQHGTAVPPGGEAGGEQCTPGKSMPFLEHLEELRRRLLKALL
                     AVVVTTCAALYFSDHIVAFMQYPLKGVKLYNIEVAGTFYAYLKIALVTGLFAAMPVVF
                     YQMWMFIAPGLYKRERAAILPLVTSSTLLFLLGAAFCFWAVLPVATTYLIGFAGDLVV
                     NTITITSYVNYVGLMMIAFGLGFQLPILAFFLAKMGVVTAALLSRIRRYAIVAIAIVA
                     AILTPPDVVSQALLGIPLYLLYEVSIVVVFFVQRGKRRKQAAEAAAMADRAPSGRAGD
                     "
     gene            2520..3653
                     /locus_tag="PKM94_11855"
     CDS             2520..3653
                     /locus_tag="PKM94_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOD67368.1"
                     /translation="MKHQAQAAAARPGIEGVRTIVAIASGKGGVGKSTVAVNLAYALA
                     HDGAAVGLMDADIYGPSQPGMLGSEGAQPTIGDNYLEPLTKHGIKFISMGLLVPDNGP
                     VVWRAPIATKMIQQFIGGVTWGNLDYLLIDLPPGTGDVQLTLAQQASLAGAVIVTTPQ
                     EVALGVARKGVRMFQQVNVPIIGVIENMSGFACGHCGQVTAVFKQGGGRAMAEEFGVP
                     YLGALPLDPEIMMSGEMGRPVVHNGAESAAAKAILQVAGEVRRRVEELAGSAGPDEAR
                     QYEVAADGNLHIVWGDGRTSRHSPYNLRVGCNCASCRDEDTGERTLDRRKVPLDIAIR
                     DVKPVGRYGLSLRFSDGHDTGIYTFSRLHTLDESGAEQAGFDV"
     gene            3673..4260
                     /locus_tag="PKM94_11860"
     CDS             3673..4260
                     /locus_tag="PKM94_11860"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOD67369.1"
                     /translation="MTDNKIAITGEPTRDPGVCRFIVDRPVFADGTVNCRSKEAAAGS
                     PLLEALFAIDGIAEVMVSGNTLTIAKTTLDPWPVLGRRIGAAIREQINSGRRLIDPAM
                     AKKTPSEEEIRRKVARLFEEQINPAISSHGGFVELHDVEGTTVYVRLGGGCQGCASAN
                     ATLRHGIERAIREEVPEVSEVRDVTDHTAGMNPYY"
     gene            4359..4886
                     /locus_tag="PKM94_11865"
     CDS             4359..4886
                     /locus_tag="PKM94_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOD67370.1"
                     /translation="MTRRLTVDPLPGFSRRVGEYAAMLEDVRRRTKTYVEGLSAPQLA
                     WHPQENVESIGTVLLHIAAVEVSYIQEDILRQPMAEAEWGIAFPIRLGIPQVSGEPLE
                     FYTERMDRVRAETLRILAELTDRDLAREVAPLDPGPSTHGPVTYTIEWILYHLIEHEA
                     HHKGQIAVMKRLVAS"
     gene            5091..7841
                     /locus_tag="PKM94_11870"
     CDS             5091..7841
                     /locus_tag="PKM94_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOD67371.1"
                     /translation="MKALLTLLAVAVVLVGSALSGSAAVPQLVSYQGRLTDSFGAPVD
                     TTVAMTFHIYNDSGGTIELWVEFQPSVVVQDGLFTVLLGAEETLGDSVFTGLVRYLGM
                     QVGNGPVGRPLIPIGSVAYAYRALTADTAKYVQNAGGDVHWTLTDGVLSTQGRWGLTR
                     GTAGNLLYGDSAHTMVNLGTAGTTGIPGQSYGFQTIAGGMWNKAGAWYATVGGGDEND
                     AGANFSTIAGGQRNKLWSMYGAVSGGFADTAQGFYSAALSGRRNVAGTSSLDTAAFVG
                     GGSDNRAQGMFATIGGGRDNKTMSSWAAVGGGWQNEASGGFSSIAGGARNAAREGGAT
                     VGGGSDNIAAFGWATIPGGQMNQAHGVYATIGGGLGNLIDGYASTIPGGCWDTVMATH
                     SYLFGIRSRLTHDSTFMVDLPHIRFGDETDGYEFPTADGDAGQVMTTDGNGRMSWGPT
                     PPGGWVNDGTRVRLGTATDSVGIGVTAPAAPLHVFGNTYGRCLIEGSSAFASLDLKIS
                     GGTTAELRKYFDGGVELATNSPSYLDLHTEEGGRVMLRVGDMHRITMDSSGRVSIGAL
                     GPKNQLDVGGATVIGRHMAGLDPGPVNGLAVEGYVGLGVINPNRRLYVADSVPGLAYA
                     LKLDNPRSTVGTDAVGMLFSVGGDGGNGYEVNRGKGALVYSLEGTYNRGSFHFLQDPD
                     DDLAICSLADAVMTIKNNGNVGVGTTNPNDKLEVEGFIRMDGGAGGGSALRFVDGGTL
                     RWALLYRPWASHKLGFFDEQAGRWTLAMEQGSGEVGIGTDSPNYTLDVRGTIGNNATL
                     YHSDRRWKKNVVALDHALDKVLRLRGVNYEWRTDEFAEMRFPTGRQVGLIAQEVEGEI
                     PEIVSTDADGYKSVDYAKLTAVLVEAVKELQKQNETLQKRIEALEGRGRTAAVTGESA
                     AR"
     gene            complement(8082..9209)
                     /locus_tag="PKM94_11875"
     CDS             complement(8082..9209)
                     /locus_tag="PKM94_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67372.1"
                     /translation="MAHAYTPGLKVTERLLVKKRRILPLKGKVVVKVGDRVQPDDVVA
                     RTELPGNVEPLNVANKLSVPPEDLDLVMVKKEGDPIVKGEPIAVKKSFLKWFSSSAAA
                     TIDGTLESISTITGQVLQRGMPIPVEVRAYISGEVIEVIPNEGCVVATDAAFVQGIFG
                     IGGETHGDIKVVVPSNDTVLDDRLITDDLRGKVVIGGSMVTAEALKKAIRAGVKGIVV
                     GGFDDKDLRDFLGYDIGVAITGSEEIGCTLVVTEGFGPIAMAEATFKLLKHHEGEMAC
                     INGATQIRAGVIRPEVVIPAAKEGRRDAAAAVSAVGGLAIGSPIRVIRHPHFGKIGRV
                     TALPSPLMKLESESKARVLEVEFGNGERATVPRANVEMIEG"
BASE COUNT         1737 a         2770 c         3300 g         1531 t
ORIGIN      
        1 gctctaccgg gagctggccg agcaggcgta aaggggggcg gggaagtcgt tgtgcggtgg
       61 cggccggtgg cgtatattcg gagcgcccgg aaccaaccgg ccggacggag cggaagcaaa
      121 aggagtaggg tgcgatgctg ttccaggtga gcatgtttcc cacgagcacg gggaaatcgt
      181 cggtgtcggc ggaggtcgcc cgggtgatcg acctgatcga ccggtccggc ctgccgtaca
      241 aactcggggc aatggcgacg gtgatcgagg gggagtggga accagtgatg gctctgctga
      301 accgggcccg ccggatgctg cgccgccggg ggcatgaacg cgtgtacatg gtgatcacga
      361 tagacgaccg caagggcgcc aaaaaccgct tggccggcaa agtgctgtcg gtcgaggaga
      421 aactgggacg tgaggtgatg aaatgaactc ggaagtgtgt gtggtgtttg tttccctgcc
      481 gcgtgacgag gcccgcaaca tggccaaggc gctggtggag aactgccttg cggcctgcgt
      541 caacatcctc ccccggatcg aatcgtattt cacctgggag ggacgggcgg agttcgacga
      601 ggaggcgctc ctggtgatca agacgacgcc ggcgcggttc gaggcgctca agggatatgt
      661 cgaggaaaac cacccgtacg aactgccgga aatcatcgcc gtgccggtca cgcaggggct
      721 gtcggaatac gtggcctggg tgaaaaagga gtcggtgagc gaggcctaac gccccggcgg
      781 cgggcgggcc gaccggccgg gccgcaacat catgaagtgc cccaactgcg gtcacgagga
      841 agacaaggtg gtggacagcc ggaagtcgca cgacgggcgg gcggtgcggc gacgccggga
      901 atgcctcaac tgcaaggcgc ggttcaccac ctacgaatat gtcgagcacc agcagatcac
      961 ggtgctgaag tcggacgggc ggcgggaacc attcgaccgg aacaaaatcg agtacggcat
     1021 caaactcgcc tgcaacaagc tccccgtcag ccgcgagcag atcaaagcca tggtcgacga
     1081 aatcgaggcc gaggtcaacg cccgcgtcgg caacgagatc cgctcggccg atatcggcga
     1141 actcgtcatg gaacggctac gggccgtcaa cgacatcgcc tacgtccgct tcgcctccgt
     1201 ctaccgccag tttcgcgaca cggcggagtt cctcgaagaa atcaagaagc tgctgaagta
     1261 gcactcagga tcggaccgtt cgcggagagg cgccggagga cggcgccggg gagcccgcga
     1321 ccgggttttc gtaggtcagg acctttacgg tcgtgacggg gccgctagca tcgatagcag
     1381 gaatgtcagg atcacaaggg tcctgaccga cggacgcccg acgatagtat acgcgtcgcg
     1441 ccggccacat gcgcagatac cctggccggc cgggaggcgg caatattcgc gttgacaagg
     1501 tgggaggtat ccgcgacatt ttgcccatta tggcagacca gcatggcacc gcagtcccgc
     1561 ccggcggcga agcaggcggc gagcagtgca cacccggcaa gagcatgcct ttcctggagc
     1621 acctggagga acttcgccgg cgtctgctga aagcgctcct ggcggtggtg gtgacgacct
     1681 gtgcggcgct gtatttttcg gaccatatcg tggcgttcat gcagtaccct ctgaagggcg
     1741 tcaagctgta caacatcgaa gtggccggga ctttttacgc ctatctcaag atcgcgctgg
     1801 tgaccggttt gttcgcggcg atgccggtgg tcttctacca gatgtggatg tttatcgcgc
     1861 ccggtctgta caagcgggag cgggcggcca ttctgccgct cgtgacctcg tccacacttt
     1921 tgttcctgct gggagcggca ttctgtttct gggcggtgct tccggtggcg accacatacc
     1981 tgatcgggtt cgccggggac ctggtggtca atacgatcac gatcacgagt tacgtgaact
     2041 atgtcgggct gatgatgatc gcgttcgggc tgggctttca gctgccgatc ctcgccttct
     2101 tcctggccaa gatgggggtg gtgacggcgg cgctcctgtc ccggatccgg cgctacgcga
     2161 ttgttgcgat cgcaatcgtg gcggccatcc tgactccgcc cgacgtcgtt tcacaggcgc
     2221 tcctggggat tccgctgtat cttctgtacg aggtgtcgat cgtggtcgtg ttcttcgtcc
     2281 agcgggggaa gcggcggaaa caggccgcgg aggcggcggc gatggccgac cgggcgccct
     2341 ccggccgggc cggggactga ccccgggccg caagccgcgg ctgacttaag tcaatgacag
     2401 cgaacttcgc ccgcgcgata ttgttctaga aaagatgact gcgggtcgcc ggacccggcc
     2461 gcgtcggcct ccgacgcaac cgggagcgcg acggccgaaa gggaaagcag gaagagccaa
     2521 tgaagcacca agcacaagca gcggccgcgc ggcccggtat cgagggtgtg cgcaccatcg
     2581 tggcgatcgc cagcggcaag gggggagtcg ggaaatcaac ggtggcggtg aatctcgcct
     2641 acgcgctcgc gcacgacggc gcggcggtcg gtctcatgga tgccgacatc tacgggccga
     2701 gccagccggg gatgctcggg tcggagggcg cccagccgac aatcggcgac aattacctcg
     2761 aaccgctgac aaaacacggc atcaagttca tctccatggg gctcttggtc ccggacaacg
     2821 gaccggtcgt gtggcgggct ccgatcgcca ccaagatgat ccagcagttc atcggcgggg
     2881 tgacgtgggg gaacctcgac tatctgctga tcgaccttcc ccccggaacg ggggatgtgc
     2941 agttgacgct ggcgcagcag gcgtcgctgg cgggcgcggt gatcgtgacg acgccgcagg
     3001 aggtggcgct cggggtggca cgcaagggcg tgcgcatgtt ccagcaggtc aacgtgccga
     3061 ttatcggcgt gatcgaaaac atgagcggat tcgcctgcgg gcactgcggg caggtgacgg
     3121 cggtgttcaa gcaggggggc ggccgggcga tggccgagga gttcggcgtg ccgtatctcg
     3181 gggccctgcc gctcgatccg gagatcatga tgagcgggga gatgggccgg ccggtggtgc
     3241 acaacggagc ggagtcggcg gccgccaaag ccattctcca ggtcgccgga gaggtccggc
     3301 gccgcgtcga agaactggcc ggatcggccg gcccggacga agcccggcag tatgaggtgg
     3361 cggccgacgg caacctgcac attgtgtggg gtgacggccg cacttcccgg cactcgccct
     3421 acaacctccg cgtgggctgc aactgcgcaa gttgccgcga cgaggacacg ggggagcgga
     3481 cgctcgatcg gcgcaaggtg ccgctcgata tcgcgattcg ggatgtgaag cctgtcggac
     3541 ggtacgggct gtcgctccgg ttcagcgacg gccacgacac ggggatctat acattctcgc
     3601 gcctgcacac cctggatgag tccggggccg agcaggccgg atttgacgtc taacaccagc
     3661 ggagcagcca ctatgacgga caacaagatt gccattaccg gcgagccgac ccgggacccg
     3721 ggggtctgcc gcttcattgt cgaccggccc gttttcgccg acgggacggt caactgccgc
     3781 tcgaaagagg cggccgcagg ctccccgctt ctcgaggccc tctttgccat cgacgggatc
     3841 gccgaggtga tggtgtcggg gaatacgctg acgatcgcca agacgaccct tgatccctgg
     3901 ccggtgctcg gccgccggat cggggcggca atccgcgagc agatcaactc cggccggagg
     3961 ctgatcgacc cggcgatggc gaagaagacg ccctcggagg aggagatccg tcgcaaggtg
     4021 gcccggctct tcgaggagca gatcaacccc gcgatttcat cgcacggcgg gtttgtcgaa
     4081 ctccacgacg tcgaggggac gactgtctat gtccgcctgg gcggcggctg ccaggggtgc
     4141 gcctcggcca atgccaccct tcgccacggg atcgagcggg ctatccgcga ggaagtgccg
     4201 gaggtgtccg aagtccggga tgtgaccgac catacggcgg gaatgaatcc ctactactga
     4261 ggccgtcgcg ccctccggcc aacaggcgcg tgcgcgagga ccgaacaccc ccccggcggc
     4321 ggcaaggccg ccggggtgtt atttgtggag gcgccggcat gacccgacga ttgaccgttg
     4381 accccctccc cggattcagc cggcgcgtgg gggagtacgc cgcgatgctc gaagatgtgc
     4441 ggcggcgaac caagacctac gtggagggat tgagcgcccc ccagcttgcg tggcacccgc
     4501 aggagaacgt ggagtcgatc ggcaccgtgc tcctccacat cgccgcggtg gaggtgtcgt
     4561 atatccagga agatatcctt cgccaaccca tggcggaggc cgagtgggga attgcttttc
     4621 cgatccggct ggggatcccg caggtctccg gtgagccgct cgagttctat acggagcgga
     4681 tggaccgggt gcgggccgag accctgcgga tcctcgcgga attgaccgac cgggacctcg
     4741 cgcgggaggt ggcgccgctc gacccgggac cgtcgacgca cggcccggtg acttacacga
     4801 tcgagtggat cctgtaccat ctgatcgagc acgaagcgca ccacaagggg cagattgcgg
     4861 tgatgaagcg gctggtcgca tcgtagggcg gcgggacggg acgcgcaggg caagcagtcc
     4921 ccttgacaaa acggacaaaa atgccgatat attggcatgg ttcctccggt tgttcgcccg
     4981 gtccgagggt ggcaaccgcc tgacaggtat cgcggggtct cagaaatcga caccgtatgg
     5041 ttgcgaacaa cccattctct tgttgagtcc ttctcatcca ggagtccatc atgaaagcac
     5101 tgcttacgct cctcgcggtt gcggttgtgc tcgtggggtc ggccctgtcc gggtcggcgg
     5161 ccgtgccgca gctggtcagc taccagggcc gtctgaccga ctcgttcggc gcgcccgtcg
     5221 acaccaccgt ggcgatgaca tttcacatct acaacgactc gggcgggacg atcgagctgt
     5281 gggtggaatt ccagccgtcg gtagtcgtcc aggacgggct gttcacggtg ctgctcggcg
     5341 ccgaggagac tctgggcgac agcgtgttta cgggcctcgt gcgatacctg ggaatgcagg
     5401 tcggcaacgg gccggtcggc cggccgctga ttccgatcgg gagcgtcgcg tacgcctatc
     5461 gcgctctgac ggcggatacg gcgaagtacg tgcagaatgc gggcggggac gtgcattgga
     5521 cgctgactga cggcgtgttg tcgacccagg ggcgatgggg gctcacgcgc ggaacggcgg
     5581 gtaacctgct ctacggcgac agcgcacaca cgatggtgaa tctcggcacg gcagggacga
     5641 ccggcatccc cgggcaaagc tacgggttcc agacgatcgc cggcgggatg tggaacaagg
     5701 cgggggcgtg gtacgcgaca gtcgggggag gcgatgagaa cgacgccggc gccaacttca
     5761 gcacgatcgc cggcgggcag cgcaacaagc tgtggagtat gtacggcgcc gtcagcggcg
     5821 ggtttgccga tacggcccag gggttttaca gtgcggccct gtcgggacgg cgcaacgtgg
     5881 ccggaaccag cagcctcgac accgcggcgt tcgtgggcgg ggggagcgac aaccgggccc
     5941 aggggatgtt cgcgacgatc ggcgggggac gggacaataa gaccatgagc tcatgggctg
     6001 cggtcggcgg agggtggcaa aatgaggcca gcggcggttt ttcaagcatt gccggcggcg
     6061 cacgcaatgc ggcgcgcgaa ggcggagcca ccgtcggagg cggcagcgac aacatcgcgg
     6121 ctttcggatg ggcgaccatc ccgggaggcc agatgaacca ggcccacggc gtctacgcca
     6181 ccatcggagg cggcctcggg aatctcatcg atgggtatgc gtccactatc ccgggcgggt
     6241 gttgggatac ggtgatggcg acgcactcct acctgttcgg catccggagc agactgacgc
     6301 acgattcgac tttcatggtg gacctgccgc acatccggtt cggcgacgaa acggacgggt
     6361 acgagttccc gacagccgac ggggacgcgg ggcaggtgat gacgaccgac gggaacggcc
     6421 ggatgagctg gggcccgacg ccgcccggcg gctgggtgaa tgacgggacc agggtgcggc
     6481 tggggacggc gaccgacagc gtggggatcg gggtaaccgc gccggcagcg ccgctccatg
     6541 ttttcggcaa cacctacggc aggtgtctga tcgaagggag cagtgcgttt gccagcctgg
     6601 atctcaagat ctccggcggc acgacggcgg aactccggaa gtactttgac ggaggagtcg
     6661 aactggccac caacagcccg tcgtatctgg acctccacac ggaggagggg gggagagtca
     6721 tgttgcgggt cggcgatatg catcgaataa cgatggacag ctcagggagg gtgagtatcg
     6781 gcgccctggg ccccaagaat cagctcgatg taggcggcgc cacggtgatc ggccggcaca
     6841 tggccggcct ggaccccgga ccggtcaacg gcctcgctgt tgagggatat gtcggcctgg
     6901 gtgtgatcaa cccgaaccgg aggctgtatg tcgccgacag cgtccccggc cttgcctatg
     6961 cactgaagct cgacaacccg cgttccaccg tcgggaccga tgccgtcggg atgttgttct
     7021 cggtcggcgg cgacgggggc aacggctacg aagtcaaccg cgggaaggga gcgctggtgt
     7081 actctctcga ggggacctac aaccgcggga gcttccactt cctccaggat cccgacgacg
     7141 acctcgccat ctgctcgctc gccgatgcgg tgatgacgat caagaacaac gggaatgtcg
     7201 gcgtggggac gaccaatccg aatgacaaac tggaggtcga gggattcatc cggatggacg
     7261 gcggtgcggg cggaggttcg gcgctgcgat ttgtcgacgg cgggacgctc cgatgggcgc
     7321 tcctctatcg gccctgggcc tcgcacaaac tcggtttctt cgatgagcag gcgggccggt
     7381 ggacgctggc gatggagcag gggtcaggcg aggtcggaat cggcaccgac agtccgaact
     7441 acaccctcga cgtacggggg acaatcggga acaacgcgac gctgtaccac agcgaccggc
     7501 ggtggaaaaa gaacgtggtc gccctcgacc acgccctcga caaggtgctc cgcctgcgcg
     7561 gcgtgaacta tgagtggcgc accgacgaat ttgcggagat gcggttcccg acaggcaggc
     7621 aggtcgggtt gatcgcccag gaagtggaag gggagattcc ggagatcgtg agcaccgacg
     7681 ctgacgggta caagtcggtt gattatgcaa agctgacggc ggtgctggtg gaggcggtca
     7741 aggagcttca gaaacaaaac gagacccttc agaagcgaat cgaggcgctg gaggggagag
     7801 gtcgcacggc tgctgtgacg ggagagtcgg ctgccaggta gagcggccgg gaaacccgcc
     7861 ggatgacgcc gcgaaaccgc tctcgcaggt caaaaccgta aaggtcctga cggggcggct
     7921 tgcgtcgatg gaaagaatgt caggaccaca agggttctga cctgcaagaa atccggaggg
     7981 ctatctctgc agatggcgaa gaatgtcagg accacaaggg tcctgacacg ccgaactgac
     8041 tggcccagcg gggaaacgac cggcccgggg cgcggcccgg gtcaaccctc aatcatctcg
     8101 acattcgccc ggggaacggt cgcgcgctcg ccgttgccga actcgacctc gagcacgcgc
     8161 gctttcgact ccgattcgag cttcattaaa ggcgagggga gagcggtgac tctgccgatt
     8221 ttcccgaagt gcgggtggcg gatgacgcga atcggggagc cgatggcgag tccgccgacg
     8281 gccgagaccg ctgccgcggc gtccctccgg ccttctttgg ccgcggggat gacgacctcg
     8341 gggcggatga cgccggcgcg gatctgggtg gcgccgttga tgcaggccat ttcgccctcg
     8401 tggtgcttga gcagcttgaa cgtcgcttcc gccatggcga tcggcccgaa gccctcggtc
     8461 acgaccagcg tgcagccgat ctcctcggac ccggtgatgg ccacgccgat atcatagccg
     8521 aggaagtcgc ggaggtcctt gtcgtcaaaa ccgccaacga cgatcccctt gacgcccgcc
     8581 cggatcgcct tcttgagcgc ctcagccgta accatcgaac cgccgatgac gacttttccc
     8641 cgaagatcgt cggtgatgag ccggtcatca aggacggtgt cgttggacgg aacgacgacc
     8701 ttgatgtcgc cgtgcgtctc gccgccgatg ccgaaaatgc cctggacaaa ggcggcgtcg
     8761 gtggcgacga cgcacccctc gttggggatg acttcgatca cctcgccgct tatgtaggcg
     8821 cggacctcga ccgggatcgg cataccgcgc tggagcacct ggccggtaat ggtcgagatg
     8881 gactcgagag tgccgtcgat ggtggcggcc gcgctgctgg aaaaccactt gaggaacgac
     8941 ttcttgacgg cgatcggctc gcctttcacg atcgggtcgc cctctttctt gaccatcacg
     9001 agatcgagat cctcgggcgg tacgctgagc ttgttggcga cgttgagggg ttccacgttg
     9061 cccggcagct ccgtgcgggc gaccacgtcg tccggctgga cccggtcgcc gaccttgacc
     9121 acgaccttcc ccttcagcgg gagtatccgc cgcttcttga cgaggagacg ttcggtgact
     9181 ttcagtccgg gcgtataggc atgagccata gacttaacct tcctttatct caaaacacat
     9241 gcgtgccggt tacgggcggc gactcttccg cacaaggagt cggatgacga tcagggccgc
     9301 ggcgaggatg agggccagcc aggcgatcca ccggagag
//
LOCUS       DAOPOE010000110         9129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8541_length_9129_cov_6.169385, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000110 DAOPOE010000000
VERSION     DAOPOE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_9129_cov_6.169385"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="hprK"
                     /locus_tag="PKM94_11880"
     CDS             <1..801
                     /gene="hprK"
                     /locus_tag="PKM94_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOD67373.1"
                     /translation="QIVGETEMAYMNRVPLERLAEISHLLFEKDIPMVIISKGLTPPP
                     EFLDAADRHRTGVFSSRLTTAELAARLSAYLDHIFAPSITVHGTLVDVYGVGLLYTGK
                     SGIGKSEVALDLIERGHRLVADDVVKITRAAPDVVKGSGSELLGHHMEIRGVGIIDIE
                     GLFGIRAIRMQKRIEVEVNLTLWSETDDYERLGLEERYTTILGVQIPIVRVPISPGKN
                     ITVISEVIAMNHMLKVYGENSALEFSKRLSQQLSRQSLTKDYLESDFE"
     gene            960..2693
                     /locus_tag="PKM94_11885"
     CDS             960..2693
                     /locus_tag="PKM94_11885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD67374.1"
                     /translation="MDKGYRPLVSAFGLLLLAGLLAGCGKSDNPTLAVVGDYEIKAAE
                     FNDYFRSIRYPFPTAQDEFAKRREMLDTIVVNRLLIQAAYEKGIDQSEELARLVVQNQ
                     DRLLVDVLAQRRIAEKSVPTEPEIREFWEKLEYKIKASHILVDNLDTANALLARIQNG
                     ESFEKLAFDYSRDPSAKKNKGDLGYFTWGSMISAFQEAAFAMAPGEVSPPVHTEYGYH
                     IIKLVDKLPNEFRDSFDKMKMEIKQQLENRKRSELSRAYVESLRTKFPIHVDTATCQY
                     LLHKRSMLYPPMLLETLPRNDFDVEQLDRSEKELVLATWDNGQITVLEYLDLAKNLSP
                     AIRPDFDQYDSLASVIFTIKLQDLLVVQAHREGMDSDPEYQRRLKLFKELTMAMIMRD
                     DSLPVPPAPDDGMVRRYYDEHLDEFTDPAKVQVFEILLSDELTAGKLAKEIRSLQGFR
                     DKAMDLTERPGKRAAGGDLGYIDRKWFPEIYDLAVKTPVGQIGGPVVTQGKYSIFYVA
                     DKISPTVKDFLDVKAQILTKLKEEQRVQALAVWIEERKAQTTIRINEDALRTTIDMDK
                     YPQGVPQGEQG"
     gene            2693..4009
                     /locus_tag="PKM94_11890"
     CDS             2693..4009
                     /locus_tag="PKM94_11890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD67375.1"
                     /translation="MAGAGRGRTPLIIVAALAALFAAGPAGAARDTVDRIVAVVGDKV
                     ILASELAGQMQLAVLQSKKQPKSEEEVEKLKREVLEEMISDQLFLVAAEQDTSVKVTD
                     EEIDQALDQQVARIAANFPTTAEFEAALSQEGLTLRDLKKRYRDDIRGQLLKQRLVGR
                     RLQSVSVSRHEVEAFYSQFRDSIPTQPEAVKLAHILLALAPAPAVDDSVKTLAAALRQ
                     RVLDGADFAALSAQYSSGGAGANGGDLGWVSRDDVVEEFARAAFSLQPGDISGVVRTQ
                     FGYHVIQCEDRRDNQVRLRHILLAVQPTREDTLQTSELADSLIAEARAGGDFAQMAKA
                     YSADDRTRATGGELGWFAVNELPVEFAAAVREWKTPGEIRGPVLTEFGIHILKLLAYQ
                     SEHEFTLENDYDRLKELARQEKTARMIEDWIDDIKARTYIDYRLGS"
     gene            4009..4257
                     /locus_tag="PKM94_11895"
     CDS             4009..4257
                     /locus_tag="PKM94_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOD67376.1"
                     /translation="MRIDDYLSTVGVVRRRTVAKELGQNGLVTVNGQKVKPAYQVRPG
                     DVIHIKGSRSVTVEVIDIPSGSVPKEQRERYYRIVAHP"
     gene            4377..5867
                     /locus_tag="PKM94_11900"
     CDS             4377..5867
                     /locus_tag="PKM94_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOD67377.1"
                     /translation="MSKTTRPATRGELKVSGWKSRSVKDELRDNLRARIESGEPVFPG
                     IVGYDKTVLPGIENALLARHNFILLGLRGQAKSRIVRGLETLLDEYIPVIPGSLLNED
                     PLSPISPRGRQMVAEMGDRMPIAWLHRSERYHEKLATPDVSIADLIGDIDPIKAAREK
                     LDISNEEVIHWGIIPRTNRGIFAINELPDLQPRIQVGLLNILEEKDIQVRGFPLRVPL
                     DMLLVFTANPEDYTNRGNIITPLKDRIDAQIITHYPRTLAEARAITEHEADIAAADIR
                     IPDLVRDLIEEVSFQARASEFVDQTSGVSARVSIAAYEALVSNIERRVLRFRDQDRFP
                     RMADVYAIIPAVTGKIELVYEGEREGSALVAHNLIGLAVKEVFARHFPEPRRERRRGD
                     QSSAADRENPYEAITDYFSSGRKLELSDDMPFDRYKKALTSIAGLDRIVRRYFDTRDE
                     REFLLRMELVLEGLHQYNVIAREIRDSAVSYGDVLSDMLRGMESDR"
     gene            5901..6995
                     /locus_tag="PKM94_11905"
     CDS             5901..6995
                     /locus_tag="PKM94_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOD67378.1"
                     /translation="MRFIYSEWNEELFRRLRQLADLMAIYNHLLIRLAGDVEETLRLM
                     RRLQEQGILDEKYDLDEFEEELRRNRLIGTGRDRRLRLTGKGERRLRQDAFDAVFQGM
                     KAAGRGNHPLPYEGGSAEEPLPERRPFEFGDSPRRIDYTSSLFNSVLRTGDLGLDMAE
                     RDLEVNEAERLASCATVLMIDISHSMILYGEDRFTPAKQVAMAFTELILTRYPKDDLT
                     VVLFGDTARQVPIEELPYVSVGPYHTNTQAGLRMARRLLMAKKHANRQIFMITDGKPS
                     MITRSNGRLYRNPVGLDPMIVNRTLDEAVICRKKKIPITTFMVTDDPYLRTFVERLTE
                     LNRGRAYFASVDDLGSFVIWDFVSHRRKRL"
     gene            7162..7428
                     /locus_tag="PKM94_11910"
     CDS             7162..7428
                     /locus_tag="PKM94_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67379.1"
                     /translation="MDGFSFDTLASLVLVLGLVFCALAYTCLSGAGKAAPWYSRGLKR
                     NHFAGRGWIFIWIAALLIVAAFVYFASEAVRGFAAGWHSRFPSR"
     gene            7630..9078
                     /locus_tag="PKM94_11915"
     CDS             7630..9078
                     /locus_tag="PKM94_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD67380.1"
                     /translation="MRRMKDSDLAASAPRGPRYGSARVSAVTRAALTLISVEGALATV
                     FTVLTGGAFLTGLALLWGANDFEIGLLTAAPFLAPIAQLASAYLIDRTGARKRITVRY
                     SVIARQSWWLIVPVALLPIGFKLEILIAVVIVCAVAINIATVGWMSWVADLVPERIRG
                     RFFGTRNVAISLATLLATMIGGIIVDYFRHRGREPVGFAVIIAAAAAFAAAAAVLLNR
                     TPDRPSRGARIGWDRLVEPLRNPTFRHLLVVFSAWNCAIGIAAPFFAPHMLTNLGMSF
                     TLVAAYSSAAAVIGMFMNRPWGALIDRFGCKPVIAFTALGIGFIPLVWLFPTAGHLGI
                     LIPEVIFSAILWTGFNLAAFNIPIANSPSGRRNAYLALFSMVTGIAFFAASLLGGYLA
                     ERWSGMTWQIGGRTFVNYHLLFAISAVLRFGSGLYALTFHEPAEKRLPVMIQFMGYSV
                     LRAVWTGRHLFPKPPRLRPKRPADPTNSKLSR"
BASE COUNT         1615 a         3317 c         2647 g         1550 t
ORIGIN      
        1 cagatcgtcg gcgagactga aatggcctac atgaaccgag tccccctcga gcgtctggcc
       61 gagatttccc acctgctgtt tgaaaaagac attcccatgg tcatcatcag caaggggctc
      121 acgccgcccc cggaattcct ggacgccgcc gaccggcacc gcaccggcgt cttctccagt
      181 cgcctcacga ccgccgaact ggccgcccgc ctctccgcct acctcgacca catcttcgcc
      241 ccctcgatca ccgtccacgg cacgctggtc gacgtctacg gcgtggggct gctgtacacc
      301 ggcaagtcgg gcatcggcaa gtcggaggtg gccctcgacc tgatcgaacg cggccaccgg
      361 ttggtggccg acgacgtggt gaagattacg cgcgcggccc cggacgtggt caagggctcc
      421 ggctccgagc tgctcgggca ccacatggaa atccgcggcg tcggcatcat cgacatcgag
      481 ggcctcttcg gcatccgcgc cattcgcatg cagaagcgga tcgaggtgga agtgaacctc
      541 accctgtggt cagagacgga cgactacgag cgcctcggcc tcgaggagcg ctacacgacg
      601 atcctcggcg tccaaatccc cattgtccgg gtccccattt cccccggcaa gaacatcacc
      661 gtcatctccg aagtcatagc catgaaccac atgctcaagg tgtacggcga aaactcggcg
      721 ctcgagttca gcaagaggct ctcccagcag ctgtcccggc agtctctgac caaagactac
      781 ctggaatcgg acttcgaata gcgccccgcc ccttcccgaa tcccctcaaa ttcccgcttg
      841 gcggccgggg cccggcccgc tataatattg tcttgtcgcc gctcggcgag aaaccaccgt
      901 gccgtcgcgc ggtacaaagg gagtatgaag cgtaggaacc tgttgaagga ataggatcga
      961 tggacaaagg ttatcgcccc ctcgtgtcgg ccttcgggct gctgctcttg gccggtctgc
     1021 tggccggctg cggaaaatcg gacaacccca ccctcgccgt cgtcggcgac tacgaaatca
     1081 aagccgccga gttcaacgac tacttccgca gcatccgcta cccgttcccg accgcgcagg
     1141 acgagttcgc caagcgccgc gaaatgctcg acacgattgt cgtcaaccgc ctcctcatcc
     1201 aggccgccta cgagaagggc atcgaccaga gcgaggaact ggcccgcctc gtcgtccaaa
     1261 accaggaccg gctgctcgtc gacgttctcg cccagcgccg gatcgccgag aagtccgtcc
     1321 ccaccgagcc cgagatccgc gagttctggg agaagctcga gtacaagatc aaggcctccc
     1381 acatcctcgt cgacaacctc gacacggcca acgccctcct cgcccgcatc cagaacggcg
     1441 agagtttcga gaagctcgcc ttcgattact cccgcgatcc ctcggccaag aagaacaagg
     1501 gcgacctcgg ctatttcacc tggggcagca tgatttcggc tttccaggaa gccgcgttcg
     1561 ccatggcgcc cggcgaggtg tccccgcccg tccacaccga gtacggctac cacattatca
     1621 agctggtcga caaactcccc aacgagttcc gcgattcttt cgacaagatg aaaatggaaa
     1681 tcaagcagca gctggaaaac cggaaacgct ccgaactctc ccgcgcctac gtcgagagcc
     1741 tgcgcaccaa gttcccgatc cacgtcgaca ccgccacctg ccagtacctg ctccacaagc
     1801 gttccatgct ctacccgccc atgctcctgg agacgctccc ccggaacgat tttgacgtcg
     1861 agcagctcga tcggagcgag aaagaactcg tcctggccac ctgggacaac ggccagatca
     1921 cggtgctgga gtacctcgac ctggccaaga atctctcccc ggcgatccgc cccgatttcg
     1981 accagtacga ctcgctggcc tccgtcatct tcaccatcaa gctccaggac ctcctcgtcg
     2041 tccaggccca ccgcgagggc atggacagcg accccgagta ccagcgccgc ctcaagctct
     2101 tcaaggagct gacgatggcg atgatcatgc gggacgattc gctcccggtg ccgccggcgc
     2161 ccgatgacgg catggtgcgg cggtactacg atgagcacct cgacgaattc acggatccgg
     2221 ccaaagtgca ggtctttgaa attctcctct ccgacgagct cacggcgggg aaactggcca
     2281 aggaaatccg ctccctccag gggttccgcg acaaggccat ggacctgacc gagcgtccgg
     2341 gcaagcgcgc ggccggcggc gacctcggct acatcgaccg caagtggttt ccggagatct
     2401 acgacctcgc cgtcaaaacg ccggtcggtc agatcggcgg ccccgtcgtt acccagggca
     2461 agtactccat cttctacgtc gccgacaaga tcagcccgac ggtgaaggac tttctcgacg
     2521 tcaaggcgca gatcctcacc aagctgaaag aagagcagcg cgtgcaggcg ctcgccgtct
     2581 ggatcgagga gcgcaaagcc cagacgacca tccgcatcaa cgaggacgcc ctccggacaa
     2641 cgatcgacat ggacaagtac ccccagggcg tgccgcaggg agagcagggc tgatggcggg
     2701 cgcgggacgc ggccgcacgc cgctcatcat cgtggccgcg ctggcggctt tgttcgcggc
     2761 gggcccggcc ggggccgcgc gcgacacggt cgaccgcatc gtcgccgtcg tcggcgacaa
     2821 ggtcattctc gcctccgagc tggccggcca gatgcagctg gccgtgctgc aatccaaaaa
     2881 gcagccgaaa tcggaggagg aggtggagaa gctcaagcgc gaggtgctcg aggagatgat
     2941 ctccgaccag ctcttcctcg tcgccgcgga acaggacacc tcggtgaaag tgaccgacga
     3001 ggagatcgac caggcgctcg atcagcaggt ggcgcgcatc gccgccaact tcccgaccac
     3061 cgccgagttc gaggccgccc tgtcgcagga gggcctcacc ctgcgcgatc ttaagaaacg
     3121 ctaccgcgac gacatccgcg gccaactgct caaacagcgc ctcgtcggcc gccgcctcca
     3181 gtcggtgtcg gtgtcgcgcc atgaggtcga ggcgttctac agccagttcc gcgactccat
     3241 cccgacccag cccgaggcgg tcaagctcgc ccacattctc ctggcccttg cgccggcccc
     3301 ggcggtcgac gattccgtga agaccctcgc cgccgcgctc cggcagagag tcctcgacgg
     3361 cgccgatttc gccgccctct cggcccagta ttcctcgggc ggcgcgggcg ccaacggcgg
     3421 cgatctcggc tgggtctccc gcgatgatgt ggtcgaggaa ttcgcccgcg ccgcattcag
     3481 tctccagccg ggcgacatct ccggcgttgt ccgcacccag ttcggctacc acgtcatcca
     3541 gtgcgaggac cggcgggaca accaggtcag gctccgccac atcctcctgg ctgtgcagcc
     3601 cacccgcgag gatacgctgc agacgagcga gctggccgat tcgctcatcg ccgaggcccg
     3661 cgcgggcggg gatttcgccc agatggccaa ggcgtactcg gccgacgacc gcacccgggc
     3721 caccggcggc gaactcggct ggttcgcggt caacgaactc ccggttgaat tcgccgccgc
     3781 cgtgagagag tggaaaaccc cgggcgaaat ccgcggcccc gtccttaccg aattcggcat
     3841 ccacatcctc aaactccttg cctaccagtc cgagcacgag ttcactctcg agaacgacta
     3901 cgaccgtctc aaagagctcg cccgccagga gaagaccgcg cgcatgattg aggactggat
     3961 tgatgatatc aaggcccgca cctacatcga ctaccgcctg gggtcgtgat gcgcatcgat
     4021 gactacctct cgaccgtcgg cgtggtcagg cgccgcacgg tcgccaagga gttggggcaa
     4081 aacggcctgg tcacagtcaa cggccagaaa gtcaaaccgg cctaccaggt ccgccccggc
     4141 gacgtcatcc acatcaaggg cagccgcagc gtcaccgtcg aagtcatcga catcccctcg
     4201 ggttcggtgc ccaaagagca gcgcgagcgc tactaccgga tcgtcgccca cccctgaccg
     4261 gtccctccgg gcgtccggtt tttccgcgtt cccccgaact tccgcccggc cgcgttgtta
     4321 tcaggaagtg ataccgaacc cggaaccgct agccccttac ggggcaaagg gaaacgatga
     4381 gcaagacaac ccgacccgcc acccgcggcg agctaaaagt gtccggctgg aagagccgct
     4441 cggtcaagga tgaattgcgg gacaacctgc gcgcccgcat cgagtccggc gaaccggttt
     4501 tcccgggcat tgttggctac gacaagactg tgctccccgg catagaaaac gccctcctcg
     4561 cccgccacaa cttcatcctt ctcggtctgc ggggccaggc gaagagccgc attgtccgcg
     4621 ggctggagac gctgctcgac gagtatatcc ccgtcatccc cggctctctg ctcaacgagg
     4681 acccgcttag cccgatctcg ccccgggggc ggcagatggt tgccgagatg ggcgaccgga
     4741 tgccgattgc ctggctccac cgcagcgagc gctaccacga gaagctcgcc acgcccgacg
     4801 tctccatcgc cgacctcatc ggcgacatcg atccgatcaa ggcggcccgc gagaagctcg
     4861 acatctccaa cgaggaggtc atccactggg gcataatccc ccgcaccaac cgggggattt
     4921 tcgccatcaa cgaacttccc gacctccagc cgcgcatcca ggtggggctc ctgaacatcc
     4981 tcgaggaaaa ggacatccag gtccgcggtt tccccctgcg cgtgccgctc gacatgctcc
     5041 tggtgttcac cgccaacccg gaggactaca ccaaccgcgg gaacatcatc accccgctca
     5101 aagatcgcat cgacgcacag atcatcaccc actacccgcg cacgctggcc gaggcccgtg
     5161 ccatcaccga gcacgaggcc gacatcgccg ccgccgacat ccgcatcccc gacctcgttc
     5221 gcgacctcat cgaggaggtg tcgttccagg cccgcgccag cgaatttgtc gaccagacct
     5281 cgggcgtctc cgcccgcgtc tccatcgctg cctacgaggc tctcgtctcg aacatcgagc
     5341 gccgcgtcct ccgcttccgc gaccaggacc gcttcccccg catggccgac gtctacgcca
     5401 tcatcccggc cgtcaccggc aaaatcgaac tggtgtacga aggggagcgg gagggttctg
     5461 cgctcgtggc ccacaacctg atcgggctgg cggtcaagga ggtctttgcc cggcacttcc
     5521 ccgagccgcg ccgggaaagg cgccgcggcg accagagctc ggcggccgac cgggaaaacc
     5581 cctacgaggc catcaccgac tacttctcgt ccggccgcaa actcgagctc tccgacgaca
     5641 tgccgttcga ccggtacaag aaagcgctga cctcgattgc cgggctcgac cgcatcgtgc
     5701 ggcggtattt cgacacccgc gacgaacggg agtttctcct gcgcatggag ctggtgctcg
     5761 agggcctgca ccagtacaac gtgattgccc gcgaaatccg cgattccgcg gtcagctacg
     5821 gcgacgtgct ctcggacatg ctgcgcggga tggagagcga ccgctgaccg ggcgccgccc
     5881 cgccgcccgg gggagaggcc atgcgattca tatactccga atggaacgag gagctgttcc
     5941 gccggctccg ccagctggcc gacctcatgg ccatctacaa ccacctgctc atacggctcg
     6001 ccggcgacgt cgaggaaacc ctccggctca tgcggcgcct gcaggagcag ggaatcctcg
     6061 atgagaagta cgacctcgac gagttcgagg aggaactccg ccggaaccgc ctcattggaa
     6121 ccggccgcga ccgccggctc cgcctgaccg gcaaggggga gcgccggctc cgccaggacg
     6181 ctttcgacgc cgtgttccag gggatgaaag cggccggccg gggcaaccac ccgctgccgt
     6241 acgagggggg ctcggcggag gagccgctgc ccgaacgccg ccccttcgaa ttcggcgaca
     6301 gcccccgccg catcgattac acgagctcgc tcttcaactc cgttctccgc accggcgacc
     6361 tcggcctcga catggccgag cgcgacctcg aagtcaacga ggccgagcgc ctcgcctcct
     6421 gtgcgaccgt cctcatgatc gacatttcgc attcgatgat cctctacggc gaggatcgct
     6481 tcactcccgc caagcaggtg gccatggcct tcaccgaact gattctcacg cgctacccca
     6541 aggacgatct caccgtcgtt ctcttcggcg acaccgcccg gcaggtgccg atcgaggagc
     6601 tgccctacgt gagcgtcggc ccctaccaca ccaacacgca ggccggccta cgcatggccc
     6661 gccggctgct catggccaag aagcacgcca accggcagat cttcatgatc accgatggca
     6721 agccgtcgat gatcacccgg tccaacggcc ggctctatcg caatccggtg ggcctcgatc
     6781 ccatgatcgt caaccgcact ctcgacgagg ccgtgatctg ccgcaagaag aagatcccga
     6841 tcaccacttt catggtgacc gatgatccct acctgcgcac gttcgtcgag cgcctcaccg
     6901 aactcaaccg gggacgcgcc tacttcgcct ccgttgacga cctcggctct ttcgtcatct
     6961 gggatttcgt gagccatcgg aggaagcggt tgtaggagcg cccgccatcg cccgccggcc
     7021 ctcccgccgt ctctcccgtc ccgaccggcc cggcgttgtc ggggtagaca aaaagagttg
     7081 acggccgcag gcgccgtccc gcttcttact tccgtacgca gcggtatgcc cggttccttt
     7141 acgcgcagcc cggagattgt catggatgga ttctcgttcg acacactcgc cagcctggtg
     7201 ctggtgctcg gtttggtctt ttgtgctctg gcttatactt gtctcagcgg ggccggcaag
     7261 gccgccccgt ggtactcccg cggtttgaaa cggaaccact tcgccggccg cgggtggatc
     7321 ttcatctgga tcgccgcgct gctgatcgtg gcggctttcg tctacttcgc ctctgaggcg
     7381 gttcggggtt tcgcggccgg gtggcactcc cggtttccgt cccgctgacc gccctttgcg
     7441 gttctgcgca tctcatccgg cgccgctgcg gcagtgatcg tgtgccgggt ggcgcgacgc
     7501 ccgccgccgt cgctcaaaaa aaaaccttcc cccgcgccct gcctgcatat cttatccccg
     7561 gcggccgagg atcccggtct tgtccgggtg cgtgtccggc cgccgaacaa gagagacccc
     7621 gggagcccca tgcgccgaat gaaagacagc gaccttgccg cgtccgcgcc gcgcgggccc
     7681 aggtacggct cggcccgcgt ctcggcggtc acccgcgcgg cgttgaccct gatttcggtg
     7741 gagggcgcgc tggccaccgt cttcaccgtc ctcaccggcg gcgcgtttct caccggcctg
     7801 gccctcctgt ggggggcgaa tgatttcgag atcgggctct tgacggcggc gccgtttttg
     7861 gcgccgatcg cgcagctcgc ctccgcctac ctcattgacc gcacgggcgc ccgcaagcgc
     7921 attaccgtcc gctactccgt catcgcccgc cagtcctggt ggctgatcgt gccggtggct
     7981 ctgctcccga tcgggttcaa gctggagatt ctcatcgccg tcgtcatcgt gtgcgcggtc
     8041 gccatcaaca tcgccaccgt cgggtggatg tcctgggtgg ccgacctcgt gcccgagcgg
     8101 attcgcgggc gtttctttgg cacccgcaac gtggcgattt ccctggcgac cctgctggcc
     8161 accatgatcg gcggcatcat tgtagactac tttcgccacc gggggcgcga accggtgggg
     8221 ttcgccgtca tcatcgccgc cgccgcggcc ttcgcggccg cggccgccgt cctgctcaac
     8281 cgcacccccg accgcccgtc gcgcggcgcc cgcatcggct gggaccgcct cgtcgaaccg
     8341 ctccgcaatc ccacctttcg gcacctcctg gtcgtcttct ctgcctggaa ctgcgccatc
     8401 ggcatcgccg ctcccttttt cgccccccac atgctcacca acctcggcat gagtttcacg
     8461 ctcgtggccg cctacagcag cgccgccgct gtcatcggaa tgttcatgaa ccgaccctgg
     8521 ggcgctctca tcgaccgctt cggctgcaag ccggtgatcg cgttcaccgc cctcggcatc
     8581 ggctttatcc cgctcgtctg gctgtttccc accgccggcc acctgggcat cctcatcccc
     8641 gaggtgatct tctccgccat cctctggacc ggcttcaatc tcgcggcctt caacatcccg
     8701 atcgccaaca gcccgagcgg ccgacgcaac gcctacctcg ccctcttctc catggtcacc
     8761 ggcatcgcct tcttcgccgc ctcgctgctc ggcggatacc tggccgaacg ctggtcgggc
     8821 atgacctggc agatcggcgg ccgcaccttc gtgaactatc atctcctctt cgccatctcc
     8881 gccgtgctca ggttcggcag cgggctgtat gccctcactt tccacgagcc cgccgagaaa
     8941 cgcctgccgg tgatgattca gttcatgggg tactcggtgc tccgcgccgt ctggaccggc
     9001 cgccacctct ttccgaaacc cccgcgcctc cgtcccaaac ggcccgccga tccgaccaac
     9061 agcaagctct cgcgttagcc tcgccggtcg gcgaatttcc cgcccgatga gaattggtgt
     9121 atctggcgg
//
LOCUS       DAOPOE010000111         9119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8556_length_9119_cov_5.153464, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000111 DAOPOE010000000
VERSION     DAOPOE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8556_length_9119_cov_5.153464"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="fusA"
                     /locus_tag="PKM94_11920"
     CDS             <1..773
                     /gene="fusA"
                     /locus_tag="PKM94_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD67381.1"
                     /translation="FKVKQDEDTAQTIISGMGELHLEILVDRLMREFGVGASVGRPSV
                     AFKETITREVEAEGRFIRQTGGKGQYGHVWIRMRPSDGGEPFRFQNKLVGGVIPREYI
                     PSVERGVREAMENGVLAGYPMTGIHVELYDGSYHEVDSNEMAFRVAGSMAFQNAARRA
                     EAVLLEPIMDVEVVAPEVYTGAVVGDLNSRRGKINGMVPREDATVIAASVPLSEMFGY
                     VNTLRNISQGRAVFTMQFARYQPVPADVSKKMLEKVKY"
     gene            792..2000
                     /gene="tuf"
                     /locus_tag="PKM94_11925"
     CDS             792..2000
                     /gene="tuf"
                     /locus_tag="PKM94_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD67382.1"
                     /translation="MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAMTMVLSRVTGTQV
                     RSFDSIDNAPEERERGITIATAHVEYESARRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKVDQVDDPELLDLVELEVRDLLSQ
                     YEFPGDDVPVIRGSALQAMTVGSDLSKPLDSAEFKSIMELMEALDTYIPEPKRDKEMP
                     FLMPIEDIFSITGRGTVATGRIERGVVKQGEEVEIVGIRETRKTVVTGVEMFRKLLDS
                     GQAGDNVGLLLRGVDKEEVERGMVLAKPGSITPHKKFKAEVYVLTKEEGGRHTPFFSG
                     YRPQFYFRTTDVTGVATLPENVEMVMPGDNVQMTVELITPIAMEKELRFAIREGGRTV
                     GAGVVAEIVE"
     gene            2024..2176
                     /gene="rpmG"
                     /locus_tag="PKM94_11930"
     CDS             2024..2176
                     /gene="rpmG"
                     /locus_tag="PKM94_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOD67383.1"
                     /translation="MPRDQIILACEVCKRRNYTTTKNKRLHPERVEFSKFCPFCNKHT
                     SHKETR"
     gene            2191..2263
                     /locus_tag="PKM94_11935"
     tRNA            2191..2263
                     /locus_tag="PKM94_11935"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2224..2226,aa:Trp,seq:cca)
     gene            2284..2475
                     /gene="secE"
                     /locus_tag="PKM94_11940"
     CDS             2284..2475
                     /gene="secE"
                     /locus_tag="PKM94_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOD67384.1"
                     /translation="MEKVAKFLKEVNAELHKVTWPTKDELIGSTIVVIVVSIIVAVFI
                     GVVDRVLTVLVQAIFGGGA"
     gene            2485..3021
                     /gene="nusG"
                     /locus_tag="PKM94_11945"
     CDS             2485..3021
                     /gene="nusG"
                     /locus_tag="PKM94_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOD67385.1"
                     /translation="MAMAWYVVHTYSGHEAKAKRYLESAIANAGLGAKFGEILVPTEE
                     VTEMRQGKRSTSTRKFLPSYILVQMDLDKVTQELVVATPGITNFVGSGGKPRPLREKE
                     VERIVGQINRSRTEEVADIPFQAGDAVKVSDGPFADFSGTVSEVNLERRKVKVMVSIF
                     GRPTPVELDFLQVELLKK"
     gene            3051..3476
                     /gene="rplK"
                     /locus_tag="PKM94_11950"
     CDS             3051..3476
                     /gene="rplK"
                     /locus_tag="PKM94_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOD67386.1"
                     /translation="MAKKVIGQIKLQIPAGQANPAPPVGPALGQRGVNIMEFCKAFNA
                     RTQDGKGILTPVIITVYSDKSFSFITKTPPASTLLRLAAKLPKGSAVPNRDKVGSVTS
                     EQLRQIAEQKMADLNAASIEAAMLMVAGTARSMGIEIKG"
     gene            3550..4254
                     /gene="rplA"
                     /locus_tag="PKM94_11955"
     CDS             3550..4254
                     /gene="rplA"
                     /locus_tag="PKM94_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOD67387.1"
                     /translation="MKRSKHYQASREKVDRTRRYALDEAIALVKGNAFAKFDESVEVA
                     IRLGVNPKHADQMVRGTVALPHGTGKKIRVAVFAAGDKAEEARVAGADIVGAEDLAEK
                     VKGGFLDFDVAVATPDMMRVLGPLGKVLGPRGLMPNPKTGTVTMDLSRAVKELKAGRI
                     EFRIDRQSNVAVSIGKVSFEANQLKENVETFIAAVARAKPASAKGTYFLSASLCSTMG
                     PGVKLDHSLLLADLKK"
     gene            4273..4836
                     /gene="rplJ"
                     /locus_tag="PKM94_11960"
     CDS             4273..4836
                     /gene="rplJ"
                     /locus_tag="PKM94_11960"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOD67388.1"
                     /translation="MAKPEKVTAVAEMKKLFDECDSFFVTDYQGLSVADVTALRKSLR
                     EQGVTFLVGKNTLLKRAAHEAGVGEIDSFLVGPTAIAFARADASAAAKVLQDSFKERE
                     LPRTKVFVVADRVYGAGDLKALAELPPREQLLAQVAAAVEAPLAELVRTIDAFFQQLV
                     GSVDALAEKRKAEGPAGPPPGEAGAAS"
     gene            4888..5277
                     /gene="rplL"
                     /locus_tag="PKM94_11965"
     CDS             4888..5277
                     /gene="rplL"
                     /locus_tag="PKM94_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOD67389.1"
                     /translation="MANPAIEEIVDKIAGLTAMDLADLSKAIQDKFGVTAAAPMAMMP
                     GMMAGGGGTAAVAEEQTEFDVVLTAVGDKKIQVIKVVRELTSLGLKEAKDLVEGAPAK
                     VKEGLSKDEAMKAKEKLEEVGAQVDIK"
     gene            5558..>9119
                     /gene="rpoB"
                     /locus_tag="PKM94_11970"
     CDS             5558..>9119
                     /gene="rpoB"
                     /locus_tag="PKM94_11970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOD67390.1"
                     /translation="MATASIIERKSYGVFRDATEMPNLLEVQLESYREFLQPDTPPDK
                     RKRQGLHQIFLDIFPITDIHENYSLEYVGYHLGPSRYTIDECRARNMTYAAPLRVTMR
                     LISRQGEGEKKEVKDIIEQDVYLGELPLLTEWGTFIVNGAERVVVSQLHRSPGVFFDE
                     DMHPNGKKLYSARIIPYRGSWVEFAMDINDVMYVYIDSRRKMPATTLLRAIGYSSDEE
                     ILNLFYKVSKVNLKGKSDEDLATYVLAETIVNTESGEVILEPGETLDEEMVEKIAAAG
                     YKAARVVDKVTNKREAYVILNTLRKDPTKSREEALMRIYALVRPGEPPTLEMAESLLE
                     KFFFNNKRYDLGEVGRYMINHRMQINIDLNRTTLDPQDFVAIVKYLVGLVNDEGYVDD
                     IDHLGNRRARTVGELMANLFSVGLSRVARTIRERLSLKDQENVTPQLLVNARTVSTVV
                     ETFFGSSQLSQFMDQTNPLSELTHKRRLSALGPGGLTRERAGFEVRDVHHTHYGRMCP
                     IETPEGPNIGLITSLATFARINKYGFLETPYRKAVNGKVTDQIDYLSADEEDRYFIAQ
                     ANEPLDKNGRFVNDKVLARRCSDYPLIPASEVQFMDVSPRQIVSVAASLIPFLEHDDA
                     NRALMGSNMQRQAVPLLRTQAPFVGTGMERKVAEDAGAVIKARRAGEVVYVDARQVVI
                     RPDVRPRINELGWVEDDVYELTKYRRSNQDTCILYKPNVALGTKVAVGDVVANGPAVE
                     NGELALGYNVLVAFMPWRGYNYEDAIILSERLMQDDIFTSVHVEEYELQVRDTKRGSE
                     EITREIPNVSEEALLNLDENGIVRIGAEVEAGDILVGKVTPKGETELSPEERLLRAIF
                     GEKAGDVRDASLKAPPGMKGVVIKTQVFSRKERTEEAKRQEKTEVAAIRKTFNHQIDQ
                     IKKLRAQRIGELLDGKTSQTVRSLVDNSVVVRAGHKYKADFFENFDFDAVVAPEGFTT
                     EANTNKHADAIVEEAARLIARKQQDMEVEVDKVVRGAELPPGVKQLVKVTIAIRRKIS
                     VGDKMAGRHGNKGVVSKLVPVEDMPHMEDGTPVDIILNPLGVPSRMNVGQILETHLGW
                     AVAKLGERVATPVFEGADIEEIKRKLRTAGLPEDGKLQLFDGRSGEPFDNPVTVGYIY
                     MLKLSHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGE"
BASE COUNT         1845 a         2485 c         3319 g         1470 t
ORIGIN      
        1 cgttcaaggt caagcaggac gaggacaccg cccagacgat catctcgggg atgggggagc
       61 tgcatctgga gattctggtg gatcgcctga tgcgcgagtt cggcgtggga gcctcggtgg
      121 gacgcccctc ggtggccttc aaggagacca ttacgcgcga ggtggaggcc gagggccggt
      181 tcatccggca gacgggcggg aaggggcagt acggacacgt ctggatccgc atgcgtccga
      241 gcgacggcgg ggagccgttc cgctttcaga ataaactggt aggcggcgtg attccccgcg
      301 agtatattcc ctcggtcgag cgcggcgtgc gcgaggcgat ggaaaacggc gtgctggccg
      361 gatacccgat gacgggcatc catgtcgagc tgtacgacgg gagctaccac gaggtggact
      421 ccaacgagat ggctttccgg gtggccggat cgatggcttt ccagaacgcc gcccgccggg
      481 cggaggcggt gctgctggaa ccgatcatgg atgtcgaagt ggtggcgccg gaggtctaca
      541 cgggcgccgt cgtcggcgac ctcaactccc ggcgcggcaa gatcaacggc atggtcccgc
      601 gcgaggacgc gacggtgatc gccgcgagcg tcccgctctc ggagatgttc gggtacgtca
      661 acaccctgcg caacatttcg cagggacggg cggtgttcac catgcagttt gcccggtacc
      721 agccggtgcc cgccgacgta tcgaaaaaga tgttggaaaa agtgaagtat tagcggaggc
      781 gagccggagt tatggcgaag cagaagtttg agcggacgaa gccgcacgtg aacgtgggga
      841 cgattggtca cgtggaccat gggaagacga cgttgacggc ggcgatgacg atggtgttgt
      901 cgcgggtgac ggggacgcag gtgcgttcgt ttgacagcat tgacaatgcg ccggaggagc
      961 gggagcgggg gatcacgata gcgacggcgc acgtggagta cgagagtgcg aggcggcact
     1021 acgcgcacgt ggactgtccg ggtcacgcgg actacgtgaa gaacatgatc acgggtgcgg
     1081 cgcagatgga cggggcgata ttggtggtga gtgcggcgga cgggccgatg ccgcagacgc
     1141 gggagcacat tttgctggcg cgccaggtgg gggttccgta catcgtggtg tacctgaaca
     1201 aggtggacca ggtggacgat ccggagttgc tggacctggt ggagttggag gttcgggatc
     1261 ttctgagcca gtatgaattt cccggggatg acgtgccggt gatccggggg agtgcgttgc
     1321 aggcgatgac ggtgggatcg gatttgagca agccgctgga cagtgcggag ttcaagtcga
     1381 taatggagtt gatggaggcg ctggacacgt acattccgga gccgaagcgg gacaaggaga
     1441 tgccgttcct gatgccgatc gaggacattt tttcgattac gggtcggggg acggtggcga
     1501 cggggaggat cgagcggggg gtggtgaagc agggggagga ggtggagatt gtggggattc
     1561 gggagacgcg caagacggtg gtgacggggg tggagatgtt tcggaagttg ttggattcgg
     1621 gtcaggcggg ggacaacgtg gggttgttgt tgcggggggt ggacaaggag gaggtggagc
     1681 gggggatggt gttggcgaaa ccgggatcga tcacgccgca caagaagttc aaagcggagg
     1741 tgtacgtgtt gacgaaggag gaggggggtc gtcacacgcc gtttttcagc gggtatcggc
     1801 cgcagtttta ttttcggacg acggacgtga cgggggtggc gacgttgccg gagaatgtgg
     1861 agatggtgat gccgggggac aacgtgcaga tgacggtgga gttgatcacg ccgattgcga
     1921 tggagaagga gcttcggttc gcgattcggg agggtggtcg gacggtgggc gccggcgtcg
     1981 tcgccgaaat tgtcgagtaa cgcggtccgc gacaggatag gatatgccga gagatcagat
     2041 catattggcg tgcgaagtgt gcaaacgccg gaactacacg acgaccaaga acaagcgtct
     2101 gcacccggag cgggtggagt tcagcaagtt ctgcccgttc tgcaacaagc acacatcgca
     2161 caaagagacg cgctagcaac cggttttgac aggccagtag ctcaactggt agagttccgg
     2221 tctccaaaac cggcggttgg gggttcgagt ccctcctggc ctgcatagag ataggtgacg
     2281 ggattggaaa aagtagccaa gtttctcaaa gaggtcaacg ccgaactcca caaggtgacc
     2341 tggccgacga aggatgagtt gatcggatcg acgatcgtgg tgattgtcgt ctcgatcatc
     2401 gtagcggtgt tcatcggggt ggtggaccgc gtgctcaccg tcctggtcca ggcgatcttc
     2461 ggcgggggag cgtgagtatt ggttatggcg atggcgtggt atgtcgtaca cacgtactcg
     2521 ggtcatgagg cgaaggccaa gcggtacctc gagtcggcca tagcgaatgc cggactgggg
     2581 gcgaagttcg gggagatcct ggtaccgacg gaagaggtga cggagatgcg gcaggggaag
     2641 cgctcgacga gcacgcgcaa gtttctcccg agctacatcc tggtgcagat ggatctggac
     2701 aaggtgaccc aggagctggt ggttgcgacg ccgggcatca ccaatttcgt cggcagcggc
     2761 ggcaagcccc ggccgctgcg ggagaaggaa gtcgagcgga tcgtggggca gatcaaccgg
     2821 agccggaccg aggaggtggc cgacatcccg ttccaggcgg gggatgcggt caaggtgagc
     2881 gacgggccgt ttgccgattt ttcggggacg gtgagcgagg tgaacctgga gcggcggaag
     2941 gtgaaggtga tggtgtcgat cttcgggcgc ccgacgccgg tggagctgga ttttctccag
     3001 gtggagttgc tgaagaaata acgatgcgat agagtggagc ggacgcgaca gtggccaaga
     3061 aagtaatagg acagatcaaa ctccagattc ccgcggggca ggcgaatccg gcgccgccgg
     3121 tcggcccggc gctcggccag cgcggggtca acatcatgga gttctgcaag gcgttcaacg
     3181 cgcggacgca ggacggcaag gggatcctga cgccggtgat aatcaccgtg tacagcgaca
     3241 agtcgttttc gttcattacg aaaaccccgc cggcctcgac cctgctgcgc ctcgcggcga
     3301 agctgccgaa gggttcggcc gtgccgaacc gcgacaaggt cggcagcgtg acctccgaac
     3361 agctccgcca gatcgcggag cagaaaatgg ccgacctcaa cgcggcctcg atcgaggcgg
     3421 cgatgctgat ggtggccggg accgcccggt cgatgggcat tgaaatcaag gggtagggac
     3481 ccccgggttc ctgccgttat tcgttaacac ctgtggcgcc cggcggcgcc gacggggaga
     3541 ataggataca tgaaacgatc caagcactac caggcatccc gcgagaaagt cgaccggacg
     3601 cgccgctacg cgctcgacga ggcgatcgcg ctcgtgaagg gcaacgcctt cgcgaagttc
     3661 gacgagtcgg tggaggtggc gatccggctg ggggtgaatc ccaagcacgc cgaccagatg
     3721 gtgcgcggga cagtggcgct gccacacggc acggggaaga aaatccgcgt ggcggtgttc
     3781 gcggccggcg acaaggccga ggaggcccgg gtcgcaggcg ccgacatcgt gggcgcggaa
     3841 gatctcgccg agaaggtgaa gggcgggttt cttgatttcg acgtcgccgt ggcgacgccg
     3901 gacatgatgc gcgtcctcgg tcccctcggc aaagtgctcg gcccgcgcgg gctcatgccc
     3961 aacccgaaga cggggacggt gacgatggac ctgagccggg cggtcaagga gctcaaggcc
     4021 gggcgcatcg agttccgcat cgaccggcag tcgaacgtgg ccgtctcaat cggcaaggtg
     4081 tcgttcgagg cgaaccaact caaagaaaat gtcgagacct tcatcgcggc cgtcgcccgg
     4141 gccaagccgg cctcggccaa agggacctac ttcctgagcg cgtcgctctg ctcgaccatg
     4201 ggtcccgggg tcaaactcga tcacagcctg ctgctggcgg atctcaagaa gtgacggaga
     4261 aggagcaatc gtatggcaaa accggaaaaa gttaccgccg tcgctgagat gaaaaagctg
     4321 ttcgacgagt gcgactcctt tttcgtgacg gactaccagg ggctgagcgt ggccgatgtc
     4381 accgcgctgc gcaagtcgct gcgggaacag ggcgtcacct tcctggtggg caagaatacg
     4441 ctgttgaagc gggcggcgca cgaggccggg gtgggggaga tcgactcctt cctggtcggg
     4501 ccgaccgcga tcgcgttcgc gcgcgccgat gcatcggcgg ccgccaaggt gctccaagac
     4561 tcgttcaagg agcgggagct gccgcggacg aaggtgttcg tggtggccga ccgggtctac
     4621 ggggcgggcg atctcaaggc gctcgccgag ctgcccccgc gcgagcagtt gctggcccag
     4681 gtggcggcgg cggtcgaggc gcctctggcc gaactggtgc ggacgatcga cgcgttcttc
     4741 cagcaactgg tcggctcggt cgacgcgctg gccgagaagc ggaaggcgga gggcccggcc
     4801 ggcccgccgc ccggagaggc cggggcggcg tcatgacgag gcaatacgaa aggtgtaact
     4861 ttacattcaa cggaagaggt acgaaccgtg gctaaccctg caattgaaga gattgtcgac
     4921 aagattgccg gactgactgc gatggacctg gcggatctgt ccaaggccat tcaggataag
     4981 tttggcgtga cggcggcggc cccgatggcg atgatgccgg ggatgatggc cggcggcggc
     5041 gggaccgcgg ccgtggccga agagcagacc gaattcgacg tcgtgctcac ggcggtcggc
     5101 gacaagaaga tccaggtcat caaggtcgtg cgcgaactca cgtcgctggg cctgaaagag
     5161 gccaaagacc tcgtcgaagg agccccggcg aaagtgaagg aaggcttgtc gaaggacgag
     5221 gcgatgaagg ccaaggagaa gctcgaggaa gtcggcgccc aggtcgacat caagtagccc
     5281 ccgggggcgt cggaaaagaa acgcccgccg ggaacaaacc gggtcccggc gggttaccac
     5341 accataccgt cagcgtgagg tcttagcgat cccatttttt tcgaattcgg ctggtggatt
     5401 gactgcagca ggtgctcccg cgaccggacg gccgggagat aagtaagact agatgacagg
     5461 atcaccgcga gcgtacaatt gaatcgtcgc caactgaccc cacatggagt cgtctgcctg
     5521 tgagggtttt cgccgttggc cataggaggg tagtggtttg gctaccgcaa gcataattga
     5581 gaggaaatcg tacggggtgt ttcgggatgc gaccgagatg ccgaacctgc tggaggttca
     5641 gctcgagtcg taccgggaat ttctgcagcc ggacacgccg ccggacaaac ggaagcggca
     5701 gggcctccat cagatcttcc tggacatctt cccgatcacg gacatccacg agaattactc
     5761 gctcgagtat gtcggctacc acctggggcc gtcgcggtac acgatcgacg agtgccgggc
     5821 gcggaacatg acgtacgcgg ccccgctgcg ggtgacgatg cggctgatca gccgccaggg
     5881 ggaaggggag aagaaagaag tcaaggacat catcgagcag gacgtctacc tcggcgaatt
     5941 gccgcttctc accgagtggg ggacgttcat cgtcaacggc gccgagcggg tggtggtcag
     6001 ccagctgcac cgcagtcccg gggtgttttt cgacgaggac atgcacccca acggcaagaa
     6061 gctgtactcg gcccggatca tcccctaccg gggttcgtgg gtcgagttcg ccatggacat
     6121 caacgacgtc atgtacgtct acatcgactc gcgccgcaag atgcccgcga ccaccctcct
     6181 gcgggccatc ggctactcct cggacgaaga gattctcaat ctgttctata aggtgtcgaa
     6241 ggtcaacctg aaggggaaga gcgacgagga cctggcgacg tacgtgttgg cggagacgat
     6301 cgtgaacacc gagtccggcg aagtgatcct ggagccgggc gagacgctcg acgaggagat
     6361 ggtcgagaag atcgcggccg ccgggtacaa ggcggcgcgg gtggtcgaca aggtgaccaa
     6421 caagcgcgag gcctacgtca ttctcaacac gctgcggaag gacccgacga agtcgcgcga
     6481 ggaggcgctc atgcgcatct acgcgctcgt gcggccgggc gagccgccga cgctcgagat
     6541 ggcggagtcg ctcctggaga agttcttctt caacaacaag cgctacgatc tcggcgaggt
     6601 cgggcgctac atgatcaacc accgcatgca gatcaacatc gacctgaacc ggacgacgct
     6661 cgacccgcag gatttcgtgg ccatcgtcaa gtacctcgtc ggcctggtga acgacgaagg
     6721 gtacgtcgac gacatcgacc acctgggcaa ccggcgggcg cggacggtcg gggagctgat
     6781 ggcgaacctg ttctcggtgg gtctgtcgcg ggtggcgcgg acgatccggg agcggctctc
     6841 gctgaaggat caggagaacg tgacgcccca gctgctcgtg aacgcgcgga cggtgtcgac
     6901 ggtggtggag acgtttttcg gctcttcgca gctctcgcag ttcatggacc agacgaaccc
     6961 gctctcggag ctgacccaca agcggcggct gtcggcgctg ggtccggggg ggctgacgcg
     7021 ggagcgggcg gggttcgagg tgcgcgacgt gcaccacacc cactacggcc ggatgtgccc
     7081 gatcgagacg ccggagggcc cgaacatcgg actgatcacc tcgctggcga ctttcgcgcg
     7141 catcaacaag tacggcttcc tcgaaacgcc ctaccggaaa gcggtcaacg ggaaggtgac
     7201 ggaccagatc gactacctgt cggccgacga ggaggaccgc tatttcatcg cccaggccaa
     7261 cgagccgctg gacaagaacg gccggttcgt gaacgacaag gtgctcgcgc ggcggtgctc
     7321 agactacccg ctgattccgg ccagcgaagt gcagttcatg gacgtctcgc cgcggcagat
     7381 tgtgtcggtg gcggcctcgc tcatcccgtt tctcgagcac gacgacgcga accgcgcgct
     7441 catgggctcg aacatgcagc gccaggcggt gccgcttctg agaacgcagg cgccgttcgt
     7501 ggggacgggg atggagcgga aggtggccga ggatgccggg gcggtgatca aggctcggcg
     7561 ggcgggcgag gtcgtgtacg tcgatgcgcg ccaggtcgtg atccggcccg acgtgcgacc
     7621 gcggatcaac gagctcgggt gggtggaaga cgacgtgtac gagctgacca agtaccgccg
     7681 ctccaaccag gacacctgca tcctctacaa gccgaatgtc gcgctgggga cgaaggtggc
     7741 ggtgggcgac gtggtcgcca acggcccggc ggtcgaaaac ggcgaactcg ccctcggcta
     7801 caacgtcctc gtcgcattca tgccctggcg cggctacaac tacgaagacg cgatcatcct
     7861 cagcgagcgc ctcatgcagg acgacatctt cacctcggtg cacgttgagg agtacgaatt
     7921 gcaggtccgc gacaccaagc gcggcagcga agaaatcacc cgcgagatcc cgaatgtctc
     7981 ggaagaggcg ttgctgaacc tggacgaaaa cgggatcgtg cggatcggcg cggaggtcga
     8041 ggccggggat atcctggtgg gcaaggtcac gccgaagggc gagaccgaac tgtcgccgga
     8101 ggagcggctc ttgcgggcga ttttcgggga gaaggcgggc gacgtccgcg acgcctcgtt
     8161 gaaagcgccg cccgggatga agggcgtcgt gatcaaaacc caggtgttca gccgcaagga
     8221 acgcaccgag gaggccaagc ggcaggagaa gaccgaggtg gcggcgatcc ggaagacgtt
     8281 caaccaccag atcgaccaga tcaagaagct gcgggcgcag cggatcggcg aattgctcga
     8341 cggcaagacg tcgcagacgg tgcggtcgct cgtcgacaac tcggtggtgg tgcgggccgg
     8401 ccacaagtac aaagccgact tcttcgagaa cttcgacttc gacgcggtgg tggcgcccga
     8461 gggtttcacg accgaggcca acacgaacaa gcacgccgat gcgatcgtcg aggaagccgc
     8521 ccggctgatc gcccggaagc agcaggatat ggaagtcgag gtcgacaagg tcgtccgcgg
     8581 cgccgaactg ccgccggggg tgaagcagtt ggtgaaggtg acgatcgcca tccgccggaa
     8641 gatttcagtc ggcgacaaga tggcgggccg tcacggcaat aagggggtgg tgtcgaaact
     8701 ggtgccggtg gaggacatgc cgcacatgga ggacggcacg ccggtggaca tcatcctcaa
     8761 cccgctcggc gttccgtcgc gtatgaacgt gggccagatt ctcgaaaccc acctcggctg
     8821 ggcggtcgcc aaattgggcg agcgggtggc gacgccggtg ttcgaaggcg ccgacatcga
     8881 ggagatcaaa cgcaagctgc ggacggccgg cctcccggag gacggcaagc tgcagctgtt
     8941 tgacggccgg tcgggcgagc cgttcgacaa cccggtgacg gtgggctaca tttacatgct
     9001 gaaactctcg cacctggtcg acgacaagat ccatgcccgg tcgatcgggc cgtactcgct
     9061 ggtgacgcag cagccgctcg gcggcaaggc gcagttcggc gggcagcggt tcggcgaga
//
LOCUS       DAOPOE010000112         9040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8658_length_9040_cov_5.671230, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000112 DAOPOE010000000
VERSION     DAOPOE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_9040_cov_5.671230"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="purB"
                     /locus_tag="PKM94_11975"
     CDS             <1..1158
                     /gene="purB"
                     /locus_tag="PKM94_11975"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOD67391.1"
                     /translation="TIAEKANFDAARIREIEAEVNHDVIAFLTSVAEHVGEDARYMHF
                     GMTSSDMLDTALSLTLKRAAALIDRKIDTALKAVRKLAFKHKTTPIMGRTHGVFAEPT
                     SVGLKFAVWYTELDRARQRVRAAADGVAVGKISGAVGNFAHLDPKIEAMVCRRLGLKP
                     APVSTQVVQRDRHAALVTALALLASSLEKFATEIRLLQRTETGEMMEGFSRKQKGSSA
                     MPHKKNPITAERICGIARLLRGYALSAMENIPLWHERDIAHSSVERVILPDACIITDY
                     GLQKFIEILNGLVIDARRMRENIYYRGGVVFSQQLLLKLAGPVGSRDTAYRIVQRNAM
                     KALETGESFRDLVRQDIEIRRYLSEKDIDRSFDLEYYTKNVDRIFERVFGK"
     gene            1161..1868
                     /locus_tag="PKM94_11980"
     CDS             1161..1868
                     /locus_tag="PKM94_11980"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOD67392.1"
                     /translation="MRDGWPFLVIGAALVLATAAGAVRWSSLGLLAAAGLCALLTLFT
                     AYFFRNPPRVVPDDPRAVVAPADGKVVLITPLEDHPALGGPATRISIFLSVFDVHINR
                     IPTAGAVDYVKYNPGRFFAAFADKASELNEQTEVGLTTPGGARLAVAQIAGLIARRIV
                     CRLRAGDTVRTGAPFGMIRFGSRTDLTIPAAWEVTVRRGEHVKGGETIMALMPAAAAR
                     PPADGFADSPREAHDSL"
     gene            1855..2646
                     /gene="pssA"
                     /locus_tag="PKM94_11985"
     CDS             1855..2646
                     /gene="pssA"
                     /locus_tag="PKM94_11985"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOD67393.1"
                     /translation="MTAYRGLVPGTFTMGNVVAGFLSILSAFEGHLIGACWFIVLAGF
                     LDALDGKVARLSGTTSQFGIELDSLADFLSFGVAPAVLVHAVKLNTLGRWGWIISIVY
                     IMAAAYRLARYNLMAESEEKKDFLGLPVPAAALTLAAYIIFSDRVWGQLEYSEWLVSM
                     IILFAFLMVSQVQYDALPDRFDTRAARAKLALLVAAAVVTIFLPRLLLFPLLALYILQ
                     GMVRELYRLGHAGVGRVTGRQNGPGDERPRYGRRKTDRMDQHGEQ"
     gene            2633..2884
                     /gene="purS"
                     /locus_tag="PKM94_11990"
     CDS             2633..2884
                     /gene="purS"
                     /locus_tag="PKM94_11990"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOD67394.1"
                     /translation="MASSKKATVYVRLKEGVLDPQGTTIHKALHQMGYQDVLSVRTGR
                     FFELELRADAADLDRTINEVCTTLLANPVIESFTVEKAG"
     gene            2881..3585
                     /gene="purQ"
                     /locus_tag="PKM94_11995"
     CDS             2881..3585
                     /gene="purQ"
                     /locus_tag="PKM94_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOD67395.1"
                     /translation="MKFGVVTFPGSNCDYDAYAAVRHVLGEEVNFLWHRSHDLMGSDV
                     IILPGGFSYGDYLRAGAIARFSPIMEKVIPFARSGGMVLGICNGFQVLTESGLLPGAL
                     LRNAHLRFACRYVYLRVEHTSSPYTRACLRGEVLRIPIAHGEGNYYHFDREIGRLEDR
                     GQVVFRYCDREGNTTPAANPNGSRNNIAGIANEEGNVLGLMPHPERAVEPILGSSDGL
                     RIFESIRATIGTTVRT"
     gene            3585..3854
                     /locus_tag="PKM94_12000"
     CDS             3585..3854
                     /locus_tag="PKM94_12000"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOD67396.1"
                     /translation="MKKREVTFILVALILGAVVGGLLGDIVAGFLPEDSEVAKVFGKS
                     IEIGVKTIQVDFYAIAFTFGLMLKVNFMSVLVLLLVIIYFRWWYL"
     gene            3892..4221
                     /locus_tag="PKM94_12005"
     CDS             3892..4221
                     /locus_tag="PKM94_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOD67397.1"
                     /translation="MPFGIGWAELLVIFAIVLLLFGARRLPEIAQGLGKGIREFKRAM
                     KDTTDEVRGSLDTEGTRDYKRAIPGSADREARRDDRERDDRARDTREQGDRERDDRPD
                     DPPDRRR"
     gene            4253..4711
                     /locus_tag="PKM94_12010"
     CDS             4253..4711
                     /locus_tag="PKM94_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67398.1"
                     /translation="MSKTSFTQADAERIAKVVAAKSVGFETDHYRFKLDNPEERRSLV
                     LEVYPETSLGKQRGMLVVVYAGNSHLQLHNCTGYVVSEDLGEVTFVAETSERLSGLVV
                     ERGAACSQYAAINRDLISSDFTQLGVAVMLSGVALSLAEEILEERKKNDR"
     gene            4878..5510
                     /locus_tag="PKM94_12015"
     CDS             4878..5510
                     /locus_tag="PKM94_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD67399.1"
                     /translation="MYRLSIDNLAKRFGSRKVFQDISIELATGQSLAVIGPNGSGKST
                     LVRCLLGLHRPSKGSVAYYETGDGEGAGRRLGEEEFRARAAFVSPYLNLYGQLSGEEN
                     IRFFAAMRGLPLTGKQINALLARVGLEGRGEDLLSEYSSGMAQRLKYAVALLGRPDFL
                     FLDEPTSNLDEAGKQIVADIVDGCRAQTIIVIATNEPEEYALAEVQCRVG"
     gene            5482..6165
                     /locus_tag="PKM94_12020"
     CDS             5482..6165
                     /locus_tag="PKM94_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOD67400.1"
                     /translation="MPRSSAGLASKSLAVFAKDIRLELRSRYALNAILMFGITTLAVV
                     SFSLGQSNLPPQLLAALYWIVMFFSAVSGLAQVFIREEESGTALALRLAAPSDPVFVG
                     KLLFNFMLLAVMTVIITPLFFIFTDAPTDGLPSFLLVLVLGVLGLCGATTLVAAIIAK
                     AAVKGALFAVLSFPVLVPLLLTVVRATQKVFDGYAAAEILTELQFLLAFAVVMIVGSA
                     LLFKFVWQE"
     gene            6315..6971
                     /locus_tag="PKM94_12025"
     CDS             6315..6971
                     /locus_tag="PKM94_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HOD67401.1"
                     /translation="MWWKLVIFAAMSGMIIAAFTTPAPQQQIGESSRIFYFHIPQAWV
                     CVLAFAVSMLYSIRYLRTRRLEDDDRALEAARLGFIFCILATVTGSIFAKVTWGSFWN
                     WDPRETSIFILLLIYGAYFALRGALEIEERRAALAAVYSIFAFLTVPFLVFVVPRMVP
                     SLHPTDSIVNENMKFTMGPAVRMIFFGSLGLFTAVFFWLFSLGRRVAHLRRRQLEYED
                     "
     gene            6978..7091
                     /locus_tag="PKM94_12030"
     CDS             6978..7091
                     /locus_tag="PKM94_12030"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOD67402.1"
                     /translation="MDGNYIALAVALAVWVGLFVYLMRVDKRVRKLEEKSK"
     gene            7088..7528
                     /locus_tag="PKM94_12035"
     CDS             7088..7528
                     /locus_tag="PKM94_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOD67403.1"
                     /translation="MNAKYLIGGVIVVIFMVWGASAFFKTTIQYVSIEEAKAADRTVQ
                     VLGKIDFEAVKYNAADSRLEFAVYDAEAADPHAAARMNVVYYGVVPGNFDQATQVVLK
                     GKSENGHFVANQMLVKCPSKYQGEGGDEYQDIQKHNQAVENSGV"
     gene            7532..>9040
                     /gene="ccsA"
                     /locus_tag="PKM94_12040"
     CDS             7532..>9040
                     /gene="ccsA"
                     /locus_tag="PKM94_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOD67404.1"
                     /translation="MTLYLSGHLLILWAFAANLVAGYGYFMAARGRTSYEQLGRRAYL
                     VLAAATVLASALLYYLFFTHNFAFKYVFEYSDSRLPFFYLVSSFWGGQEGTYLLWLLF
                     SVAIGFAVRRTAGRYASWAMVFYALVNLFFTGMMLKVSPFALLDFSAADGAGLNPLLQ
                     DPWMVVHPPVMFVGYALTGAPFALALAALVRRDFSEWVRRAAPWVVLTVLFLAAGNIL
                     GGYWAYKTLGWGGYWAWDPVENSSLIPWFVALALLHGLALERRTGALRRTNLLLAAFT
                     FWLVVCGTFMTRSGVLSDFSVHSFVDLGQNEWLIVFLLLYAGVTLGVFLRGMRSTGYV
                     PINFNFFGREFSLVGAMLLLFAFSLIVLFWSSLPILTKIFGAQPRAADIATYNAFALP
                     IAVLYALLLILSPLSSHRPQAPAKWRIHLTAGGLVSLAVALMLFVLHEGSGVAFVVAG
                     TLVATGLTVAFGQRDLRGSLIPAAALFVLALATALALGVRDGLYLLFFATAAA"
BASE COUNT         1431 a         2876 c         2982 g         1751 t
ORIGIN      
        1 acgatcgccg agaaggcgaa tttcgacgcc gcccgcatcc gcgaaattga ggccgaggtc
       61 aaccacgacg tgattgcgtt cctcacctcg gtggccgagc atgtcggcga ggacgcccgc
      121 tacatgcact tcggcatgac ctcctccgac atgctcgaca cggcgctgtc gctgacactc
      181 aaacgggcgg ccgcgctgat cgaccggaag atcgatacgg cgctcaaggc ggtccgcaag
      241 cttgcattca agcacaagac gacgccgatc atgggccgga cccacggtgt attcgccgag
      301 ccgacctcgg tcgggctcaa gtttgcggtc tggtacacgg aactcgaccg ggcccgccag
      361 cgggtgcgcg cggcggccga cggggtggcg gtcggcaaga tctccggggc ggtggggaat
      421 ttcgcccacc tcgacccgaa gatcgaagcg atggtgtgcc ggcggctcgg cctgaaaccg
      481 gcgccggtct cgacccaggt ggtgcagcgc gaccgccacg ccgcgctggt gacggcgctg
      541 gcgctcctcg cctcgtcgct ggagaagttt gccaccgaga tccgtctcct ccagcgcacc
      601 gagacggggg agatgatgga ggggttcagc aggaagcaga aggggtcatc ggcgatgccc
      661 cacaagaaga acccgatcac ggccgagcgc atctgcggca tcgcccgcct gttgcgcgga
      721 tacgcgctga gcgcgatgga gaacatcccc ctctggcacg aacgcgacat cgcccactcc
      781 tcggtcgaac gcgtgattct ccccgacgcc tgcatcatca ccgactatgg tctgcagaaa
      841 ttcatcgaga tcctcaacgg tctcgtgatc gacgcgcggc gcatgcgcga gaacatctac
      901 taccgcggcg gcgtcgtctt ctcgcagcag ctcctcctca agctcgcggg gccggtcggc
      961 tcgcgcgaca ccgcctaccg gatagtgcag cgcaacgcga tgaaggcgct cgagacggga
     1021 gagtcgttcc gagacctggt caggcaggac atcgagatcc gccggtacct ctcggagaaa
     1081 gacatcgacc ggagtttcga cctggagtac tatacgaaaa acgtggaccg catattcgaa
     1141 cgggtgtttg ggaagtgatt gtgcgcgacg gttggccgtt tcttgtgatc ggagcggcgc
     1201 tcgtgctggc gacggcggcg ggggcggtgc gctggagcag cctcgggctg ctggcggcgg
     1261 ccggcctgtg cgctctgctt accctgttca ccgcctattt cttccgcaac cccccgcggg
     1321 ttgttcccga tgacccgcgg gcggtggtcg cccccgccga cggcaaagtc gtgctcatca
     1381 ctcccctgga ggatcacccc gccctcggcg gcccggccac gcgcatctcg attttcctga
     1441 gcgtgttcga cgtccacatc aaccgtatcc cgaccgccgg cgcggtcgac tacgtgaagt
     1501 acaaccccgg tcggtttttc gcggcctttg ccgacaaagc atccgaactc aacgagcaga
     1561 ccgaggtcgg cctgaccacg ccgggggggg cgcggctggc ggtggcgcag atcgccgggc
     1621 tgatcgcccg ccgcatcgtc tgccggctcc gcgccgggga cacggtgcgc accggcgcgc
     1681 cgttcggcat gatccgtttc gggtcgcgga ccgatctcac gattcccgcc gcctgggagg
     1741 tgacggtgcg ccgcggggag cacgtgaagg gcggggagac gatcatggcg ctcatgcccg
     1801 cggcggccgc ccggcctccc gcggacgggt tcgccgacag cccaagggag gcgcatgaca
     1861 gcctatagag gtctggtccc cggcacgttc accatgggga acgtcgtggc cgggttcctg
     1921 tcgattctct cggcgttcga gggtcacctg atcggggcct gctggttcat cgtgctggcc
     1981 gggtttctcg acgctctcga cggcaaggtc gcccggctct cggggacgac cagccagttc
     2041 ggcatcgagc tcgattcgct ggccgatttc ctctcgttcg gggtggcgcc ggcggtgctc
     2101 gtccacgcgg tcaagctgaa cacgctgggc cgatggggat ggattatctc gatcgtctat
     2161 atcatggcgg ccgcctaccg cctggcccgc tacaatctca tggccgagtc ggaggagaag
     2221 aaggatttcc tcggtctgcc ggtgccggcg gcggccctga ccctggccgc ctacatcatt
     2281 ttcagcgacc gcgtctgggg ccagctcgag tacagcgagt ggctggtgtc gatgatcatc
     2341 ctgttcgcct tcctcatggt ctcgcaggtg cagtacgacg cccttccgga ccgcttcgac
     2401 acccgcgcgg cccgggccaa actggcgctc ctcgtggccg cggcggtggt gaccatattc
     2461 ctcccgagac tcttgttgtt tccgcttcta gccctctata tattgcaggg catggtgcga
     2521 gaactctacc gcttgggcca cgccggggtc ggccgggtga ccggccggca gaacgggccg
     2581 ggcgacgagc ggccccggta cggacggcga aagacggaca ggatggatca acatggcgag
     2641 cagtaagaag gcgacagtgt acgtgcggtt gaaagaaggc gtgctcgatc cgcagggcac
     2701 gaccatccac aaggcgctgc accagatggg gtaccaggac gttctgtcgg tgcggaccgg
     2761 ccggtttttc gaactggaac tccgcgccga cgccgccgac ctcgaccgca ccatcaacga
     2821 agtgtgcacc acgctgctgg ccaacccggt cattgagagt ttcacggtgg agaaagcggg
     2881 atgaaattcg gcgtcgtcac tttccccggc tccaactgcg actacgatgc ctacgcggcg
     2941 gtccgccacg tgctcggcga ggaggtcaac ttcctgtggc accggtcgca tgacctgatg
     3001 ggcagcgacg tgatcatcct gccgggcggt ttctcctacg gcgactacct gcgggccggc
     3061 gccatcgccc ggttctcgcc gatcatggag aaggtgattc ccttcgcccg gtcgggcggg
     3121 atggtgctgg ggatctgcaa cgggttccag gtgctcaccg agagcggcct cctcccgggg
     3181 gcgctcttgc gcaacgccca cctccgcttc gcctgccgct acgtgtacct gcgggtcgag
     3241 cacaccagca gcccctacac gcgcgcctgc ctgcgcggcg aggtgctcag gatcccgatc
     3301 gcccacggcg aggggaacta ctaccatttc gaccgcgaga tcgggcggct ggaagaccgg
     3361 gggcaggtgg tgttccgcta ctgcgaccgg gagggaaaca cgacgccggc ggccaatcct
     3421 aacggctcac gcaacaacat cgccggcatc gccaacgagg aagggaacgt cctcgggctg
     3481 atgccgcacc cggagcgggc ggtggagccg atcctcggct cgtccgacgg actgagaata
     3541 ttcgaatcga tccgagcaac gatcggaaca acggtgcgga cctgatgaag aaacgcgagg
     3601 ttacctttat cctggtggcg ctcatcctcg gcgccgtcgt cggcggcctc ctcggcgaca
     3661 tcgtcgccgg tttcctgccg gaggactcgg aagtcgcgaa agtattcggc aaatcaatcg
     3721 agatcggggt caagacgatc caggtcgact tctacgccat cgcctttacg ttcggcctga
     3781 tgctcaaagt caatttcatg tccgtgcttg tgcttctgct cgtcatcata tactttaggt
     3841 ggtggtatct ctagacacgg ccaccggaac tctggaaagt gagcggttaa catgccgttt
     3901 ggaatcggtt gggcggaact gctggtcatc ttcgcgatcg tcctgctcct gttcggggcg
     3961 aggcggttgc cggagatcgc ccagggcctg ggcaaaggca tccgcgaatt caagcgggcc
     4021 atgaaagaca cgaccgacga ggtcaggggt tcgctggaca ccgagggtac gcgggattac
     4081 aagcgcgcca ttccgggcag cgccgaccgc gaggcccgcc gcgacgaccg cgagcgggac
     4141 gatcgcgcgc gcgacacccg cgaacagggc gaccgcgagc gggacgaccg ccccgacgac
     4201 cccccagacc gccggcgcta gcccgggccg agcggagaaa gcggtaacag ccatgagcaa
     4261 gacatcgttc acacaggcgg atgccgagag gattgcgaag gtggtggcgg ccaagtcggt
     4321 cggattcgag accgaccact accgcttcaa actcgacaac cccgaggagc ggcgctccct
     4381 cgtcctcgaa gtctatccgg aaacgtcgct cggcaaacag cgcgggatgc tcgtggtcgt
     4441 gtacgccggc aactcccacc tgcagttgca caactgcacc gggtacgtgg tatccgagga
     4501 tctcggcgag gtcacgttcg tggccgaaac gtccgagcgc ctctccgggc tggtcgtcga
     4561 gcgcggcgcc gcctgctcgc agtacgcggc gatcaaccgc gacctcattt cctccgattt
     4621 cacgcagctc ggcgtggccg tgatgctctc cggggtggct ctctcgctgg ccgaggagat
     4681 tctcgaggag cgcaagaaga acgaccggtg aggcggccgg gacagaatcg gcgccggccg
     4741 aagccccgga ggcttcggct ttttcgtgcc gcggcggcgg actcatgcgg gtccgcccgg
     4801 accatcgccg cgccgccgga cacccgccgc cgcacctttc cgcttgcatc ccggatcgga
     4861 ttccccaaat tgcccccatg taccggctgt ccattgacaa cctggccaaa cggttcgggt
     4921 cccgaaaggt gttccaggac atctcgatcg agctggccac cggccaatcg ctggcggtga
     4981 tcggccccaa cggctcgggc aaatcgaccc ttgtgcgctg cctgctcggg ctccaccgcc
     5041 cgagcaaggg atcggtggcg tactacgaga ccggggacgg ggagggggcc ggccgccggc
     5101 tgggcgagga ggaattccgg gcgcgggccg ccttcgtgtc gccctacctc aatctctacg
     5161 gccagttgtc cggagaggag aatatcaggt tctttgccgc gatgcgcggg ctcccgctca
     5221 ccggcaagca gatcaacgcc ctcctggcgc gggtgggcct cgaagggcgc ggggaggacc
     5281 tcctctccga atactcctcg ggcatggccc agcgtctcaa gtacgccgtg gctctcctcg
     5341 gccggccgga tttcctcttc ctcgacgagc cgacctctaa cctcgacgag gcggggaagc
     5401 agatcgtggc cgacatcgtg gacgggtgcc gcgcacagac cattatcgtg atcgccacca
     5461 acgaaccgga ggagtacgcc cttgccgagg tccagtgccg ggttggctag taagtcgctc
     5521 gcggtctttg ccaaagacat ccggctggag ttgcgcagcc ggtacgcgct caacgcgatt
     5581 ctcatgttcg ggatcacgac gctggcggtg gtgtctttct cgctggggca gtccaatctg
     5641 ccccctcagc tgcttgcggc cctgtattgg attgtgatgt tcttctcggc cgtgtcgggg
     5701 ctggcgcagg tctttatccg ggaggaggag tcggggaccg ccctggcgct gcgactggct
     5761 gctccctccg atccggtgtt tgtcggcaag ttgctgttca acttcatgtt actggcggtg
     5821 atgacggtga tcatcacccc cctgttcttc atttttaccg atgccccgac cgatggtctg
     5881 ccatcgttcc tcttagtgct cgtgctcggc gtgctcggtt tgtgcggggc gacaacgctg
     5941 gtggcggcga tcattgccaa ggcggcggtg aagggggcac tctttgcggt gctgtcattt
     6001 ccggtgctgg tgccgctctt gctcacggtg gtgcgggcga cgcagaaggt tttcgacggt
     6061 tatgcggcgg cggagattct gaccgaactg cagtttttgc tggcgtttgc cgtggtgatg
     6121 atcgtcggat ccgccctcct gttcaaattc gtgtggcagg agtaaatgga gggggaccgg
     6181 tcgccgtgcg ggaaaagccc ccggcctggc gcgggccggg caatcgttga ctaaagggct
     6241 gaattttatt gattattgtc ggcccgcgcg tatcttaggc cgtacaaaca ggttgatagg
     6301 attgaagata cctcatgtgg tggaaactgg tcatatttgc ggcgatgtcg gggatgatca
     6361 tcgccgcttt caccaccccc gccccccagc agcagatcgg ggagtcctcg cggatattct
     6421 atttccatat cccccaggcc tgggtgtgcg tgctggcgtt tgcggtatcg atgctctact
     6481 cgatccgcta tctccggacg cggcggctcg aggacgacga ccgggcgctc gaggccgccc
     6541 ggctgggatt catcttctgc atcctggcga ccgtgaccgg gtcgattttc gccaaagtca
     6601 cctggggatc gttctggaac tgggatccgc gggaaacttc gatcttcatc ctgctgttaa
     6661 tctacggagc ctattttgcg ctgcgcggcg cgctcgaaat tgaagagcgg cgggcggcgc
     6721 tggcggcggt gtacagcata ttcgctttcc tgacggttcc gtttctggtg ttcgtggtgc
     6781 cgcggatggt gccctcgctg catccgaccg actcgatcgt gaatgagaac atgaagttca
     6841 cgatggggcc ggcggtgcgg atgatcttct tcggctcgct cgggttgttc acggccgtgt
     6901 ttttctggct gttcagcctc gggcggcggg tggcgcacct gcggcgaagg caactggagt
     6961 acgaggatta gacgctgatg gacggcaatt acattgcgct cgcggtggcc ctggcggtct
     7021 gggtagggct gttcgtttat ctcatgcggg tcgacaagag ggtccgcaag ctggaggaga
     7081 aatcgaaatg aacgccaagt acctgatcgg cggagtcatc gtcgtgatct ttatggtctg
     7141 gggggcctcg gccttcttca agaccaccat tcagtacgtg tcgatcgagg aggccaaggc
     7201 ggccgaccgc accgtccagg tgctggggaa gatcgatttc gaggccgtga aatacaacgc
     7261 cgcggactcc cgcctcgagt tcgccgtgta cgacgccgag gcggccgatc cgcacgcggc
     7321 cgcgcgcatg aatgtcgtgt actacggagt ggtgccgggg aatttcgatc aggccaccca
     7381 ggtcgtcctc aagggcaaga gcgagaacgg ccatttcgtc gccaaccaga tgctggtcaa
     7441 gtgcccctcg aagtaccagg gggaaggggg cgacgagtac caggatattc agaagcacaa
     7501 ccaggcggtc gagaattccg gggtctagcg gatgacgctg tacttgtccg ggcacttgct
     7561 gattctctgg gcgttcgccg ccaacctggt ggcggggtac ggctatttca tggctgcgcg
     7621 aggacggacg tcctatgagc agctcggacg gcgcgcgtat ctcgtgctgg ccgccgccac
     7681 cgtgctcgcc tccgccctcc tgtactacct cttcttcact cacaatttcg ccttcaaata
     7741 tgtcttcgag tactcggaca gccggctccc cttcttctac ctggtctcgt cgttctgggg
     7801 cgggcaggag gggacctacc tgctgtggct gctgttcagc gtggcgatcg ggttcgccgt
     7861 gcggcggaca gcggggcgct acgcctcgtg ggcgatggtg ttctatgcgc tggtgaacct
     7921 gttcttcacc ggcatgatgc tgaaagtatc ccccttcgcc ctgctggatt tctctgcggc
     7981 cgacggggcg gggctcaatc cgctgctgca ggacccgtgg atggtggtgc atccgccggt
     8041 gatgtttgtc gggtatgctc tcaccggggc gccctttgcg ctggcgctgg cggcgctggt
     8101 gcggcgcgat ttcagcgagt gggtgcgccg cgccgcgcca tgggtcgtcc tcaccgtgct
     8161 gttcctcgcg gccggcaata tcctgggcgg ctactgggcc tacaagacgc tcgggtgggg
     8221 cggctactgg gcctgggacc cggtcgagaa ttcctccctc atcccctggt tcgtggcgct
     8281 ggcgctcctg cacggcctgg cgctggagcg ccgcaccggc gcgttgcgcc gcaccaacct
     8341 cctgctcgcg gccttcacgt tctggctcgt cgtctgcggc accttcatga cccgctcggg
     8401 cgtcctgtcg gacttctccg tccacagctt cgtggacctc ggtcagaacg agtggctcat
     8461 tgtcttcctc ctgttgtatg cgggggtcac gctcggggtg ttcctgcgcg ggatgcgcag
     8521 caccggctac gtgccgatca atttcaactt tttcggccgc gagttctcgc tcgtcggggc
     8581 gatgctgctg ctgttcgcct tttcgctgat cgtcctgttc tggtcatcgc tgccgatcct
     8641 gacgaagatt ttcggcgcgc agccgcgcgc ggccgacatc gccacctata acgctttcgc
     8701 ccttccgatc gcggtgcttt atgccctctt gctgatcctc tcgccgctct cgagccaccg
     8761 gccacaggcg ccggcaaagt ggcggattca cctcactgcg ggcgggttgg tctcgctcgc
     8821 ggtcgccctg atgctcttcg tcctgcacga ggggtccggg gtcgctttcg tggtcgcagg
     8881 cacgctcgtg gccaccggcc tcaccgtcgc gttcggccaa cgcgacctgc gcgggagtct
     8941 gattcccgcg gcggcgctgt tcgtgcttgc gctggcgacc gcgctggcgc tgggcgtgcg
     9001 cgacggcctc tacctgctgt tcttcgccac cgcggcggcc
//
LOCUS       DAOPOE010000113         8879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_8849_length_8879_cov_5.585675, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000113 DAOPOE010000000
VERSION     DAOPOE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8849_length_8879_cov_5.585675"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..918
                     /locus_tag="PKM94_12045"
     CDS             175..918
                     /locus_tag="PKM94_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67405.1"
                     /translation="MSEPAVAIEHLTRRFGEFTAVDDVSLTVPRGETFGFLGANGAGK
                     TTTIRMLTGLLLPTAGAGTVAGWDIFTQSEQIKRSIGYMSQRFSLYPDLTGRENLRFY
                     GAAYGLAPGRVNRRLGELTERLGLGEFIDRQSGALPTGWRQRLALGAALLHEPSILFL
                     DEPTSGVDPVFRRRFWEILYELAAEGVTVFVTTHYMDEAAYCDRISIMHRGRIIEVGK
                     PFDIVAAHGRETLEDAFVHLIAAQEGAGA"
     gene            911..2017
                     /locus_tag="PKM94_12050"
     CDS             911..2017
                     /locus_tag="PKM94_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD67406.1"
                     /translation="MHKIRFIARKELYHILRDPRSLIIVLAMPILMTFLYGYAINLDI
                     EHITLAVLDYDRTAGSRDLIEAFYGSSYFSPPETPVRPEDPEAVLKSAEAHAVLVIRP
                     GFAEALRTGQSFDLGLYIDGSDNNMAAAVQNYAEALLLAGPYAGRADGRAPGGITLSR
                     QVLYNPDLKSAQFFVPGLIALILVMISALLTSITIAREKETGTMEQLLTAPVTPVQIL
                     VGKIVPYIFIALLDAAAIIVIARTVFGVPFAGSRLLMLGYALVYIGVSLSLGILISSL
                     AKTQQVAMIFAVTTTMLPSVMLSGFIFPIKNMPVVLQGLSYIIPARYFVRIIRAVMHK
                     GAGMEALVLEGLALAVLMVVLMAVAARKFRMKVA"
     gene            2020..3120
                     /locus_tag="PKM94_12055"
     CDS             2020..3120
                     /locus_tag="PKM94_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD67407.1"
                     /translation="MDTRALRGLVRKEFIQVFRDRNMLLIIFAMPVVQLIIFGYVANT
                     EVREVALDVYDFDRTAVSRQYATALGAGNYFTVQSPEAPLLEVEERFRRSDAEMALVI
                     PPNFTRDLTSGGRPVVGIVADGANSNTAAIGMGYLARISAQYARGVTEVSAPVRLRFQ
                     VLYNPEMESVYFMVPGIVAALLTMVTVMLTSMAVVRERERGTLEQLLVTPISTVTLLL
                     GKLLPFAVLGIVEMILALTIGVLWFGIPFAGSVPLLFALSGLYLFSTLGIGLFFSTIT
                     STQQQAMFFAWFFSVFALLTSGLFTPISNMPQSVQYLTYVNPMRYFMDIARGIMMRGA
                     GVGELAGDIWPLAVFGAVVLSVSIMRFSKRTA"
     gene            3242..3454
                     /locus_tag="PKM94_12060"
     CDS             3242..3454
                     /locus_tag="PKM94_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD67408.1"
                     /translation="MPIYEFKCGRCAATFEELVSSGTDAVPCPKCGSADVYRLISLVS
                     SKGMSAGSCAGCAPSPAKCRGCSGGH"
     gene            complement(3451..4842)
                     /gene="hemG"
                     /locus_tag="PKM94_12065"
     CDS             complement(3451..4842)
                     /gene="hemG"
                     /locus_tag="PKM94_12065"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOD67409.1"
                     /translation="MSSSISKDLAIIGGGISGLAALHFARTLRPALAAALYEKEARLG
                     GTVGTDRVAGYSFDWGPNGFLDREPLTLRLAGELGLEDRLERASAAVQKRFILRGGRL
                     REVPMSPPAFLRSDILSLRGRLRVAMEPFGRKRPEGIDESVYDFARRRIGREAADYLV
                     QPMVSGVYGGMADRLSLRATFRLMAEMEAEYGSLLRAMVAKMRATKRSGRAAGGPAGP
                     GGWLTSFRGGLDAVIARARERYGDDIATGAGAVCIERDGRGYRVGLADGREVRAPRVI
                     LATPSYEAAALVRPLSAKLSEALAAIEYAPIAVVCSGYDGAAVGRAVDGFGFLVPRVE
                     GRRILGSIWTSSIFADRAPAGKVQFRTMVGGDGDREVMNLTDEELQETVHRDLSAVMA
                     LRGGPEVIRIYRWERGIPQFTIGHLARLALIEAELARLGNIAVTGNAYYGIGLNDCVK
                     QSFRAVGELPERL"
     gene            complement(4823..5782)
                     /gene="hemH"
                     /locus_tag="PKM94_12070"
     CDS             complement(4823..5782)
                     /gene="hemH"
                     /locus_tag="PKM94_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOD67410.1"
                     /translation="MAFSETPACVILLAMGGPRTTAEVPAYLYNIFSDRRIIRLPGGP
                     LLQKPLAKLIAARRAEKVKRHYDLIGGGSPLLHWTEAQKAHLERSFAEGAVTRPLRCY
                     IGMRYAPPMTGEAVRQAYEDGCRTMFFLPMYPQYCRATTGSSFAEARRALERYDDVHP
                     VFIDDFHNNQGYLFLLREYIGNHMREDETILFSAHAIPQSFVDDGDPYVDQVRATASL
                     AAGDREYFLSFQSRTGPVAWVGPDTVAEARRLLGAGRRLFVVPISFVCDHIETLYEID
                     IELKQLLNAGERIRRMPMFNDDPRLARTLARIIDARRNVEQHL"
     gene            complement(5783..7183)
                     /gene="hemN"
                     /locus_tag="PKM94_12075"
     CDS             complement(5783..7183)
                     /gene="hemN"
                     /locus_tag="PKM94_12075"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOD67411.1"
                     /translation="MADHAHSATHVPIELLRKYDRPGPRYTSYPTAPEWSEAVGREAY
                     VKALEAAGAQTDTPLSVYCHIPFCRKRCFYCGCNTCILNEREAVGAYLAALGRETERV
                     GAHLGGRRTVNQLHFGGGTPTYLTVDELGNVLGSLGSVFRYADDCEKSIEIDPRVTTP
                     EQLSFLAGQGFNRVSLGVQDFNEAVQTAVGRIQPYRLVAETVAHCRALEFKGLNFDLI
                     YGLPKQTVASFAETIRLALSLRPDRVAVYSFAYLPQRMANQRAIKPDDLPDTEVKYGL
                     FATAVEMFTGAGYRQIGMDHFALPEDELARAQADGKLHRNFMGYTVKTSPDMIGLGMS
                     SIGYLNDSFFQNHSTLAAYHAAMDGEAFAVYRGLVLSRDDLIRQYVIAGLMCNFRLGY
                     RALRDRFGADYAEYFGAEHSRLREFFDDGLVEEGEGELRVTPLGRTFVRNIAMTFDAY
                     LGAQPSGERPQFSRTI"
     gene            complement(7189..8211)
                     /gene="hemE"
                     /locus_tag="PKM94_12080"
     CDS             complement(7189..8211)
                     /gene="hemE"
                     /locus_tag="PKM94_12080"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOD67412.1"
                     /translation="MDLANSPFLQACSGRNDGRIPVWIMRQAGRYLPDYRAVRRQATF
                     EQLCRDPKLIAEVVRQPVRRFGLDAAILFSDILIVLDPMGAAVSFAEGGPELARPIAR
                     PEDAERLHDFDVAAELAFVCDGIREIKRLLPETPLIGFAGSPFTIACYLIEGKGSRHF
                     ETVKQFIHRHPEAAERLMALVTDVTVRYLEAQLAAGCDAVQLFDSWGGILSREDYRDW
                     SLRWTEEIFTRLAPRTAPRILFVNNLAPYIDMVSALDCEVVGVDYRMDLQAAAAALPG
                     KAVQGNLEPAVLFGTRETIIARVRSILDKIEDPRRLIFNLGHGIRPETPVEAVETLVQ
                     TVHDYR"
     gene            complement(8202..>8879)
                     /locus_tag="PKM94_12085"
     CDS             complement(8202..>8879)
                     /locus_tag="PKM94_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD67413.1"
                     /translation="EGIPANNGLLIQRHEYMRLLRALTEKHGALLVLDEVITGFRLGL
                     GGALQYYGIHPDLVTFGKVIGGGMPVGAFGGRAEIMDLLSPDGPVYQAGTLSGNPVAM
                     TAGLATLEKLADGRIYADLEARNRRFVSAITARVRGNRVNVVGVASIFWIVFQEHIPR
                     AARAIDPDGIAHYNRFHGKVLDAGLYLPPSGYEVCFISAAHDDALLDGAAETLAELIK
                     EEAHTWT"
BASE COUNT         1379 a         3114 c         2868 g         1518 t
ORIGIN      
        1 gcccgaggtg agcgcccagc gcttcggcgc cggcctgcac ctgtatctgc cgcggggcgg
       61 ccgcctcgag ccccacttgc ccgctctggc ggcgtgcggc attcggccgg agcagatccg
      121 gaagatccgt cccgacctcg aagacgcctt catccagctg atggagcgat cggcatgagc
      181 gaaccggcgg tcgcgatcga gcacctgacc cggcgtttcg gggagttcac ggcggtcgac
      241 gacgtctccc tcaccgtgcc gcgcggcgag acgttcggtt ttctcggcgc caacggcgcg
      301 gggaagacga ccacgatccg catgctgacc ggcctgctcc tgccgaccgc gggcgccggc
      361 acggtcgccg gctgggatat tttcacccag tccgagcaga tcaagcgctc catcgggtac
      421 atgtcgcaac ggttctccct ctacccggac ctgaccgggc gggagaacct ccggttttac
      481 ggggcggcct acgggctcgc gccgggccgg gtgaaccgcc gcctcgggga gctgaccgag
      541 cgcctcgggc tgggcgaatt catcgaccgc cagagcggcg cgctgccgac cggctggcgc
      601 cagcgcctcg cgctcggcgc cgcgctgctg cacgaaccga gcatcctgtt cctcgacgag
      661 ccgaccagtg gcgtcgaccc cgtcttccgc cgccggttct gggagatcct ctacgaactg
      721 gccgccgagg gcgtcaccgt cttcgtcacc acccactaca tggacgaggc cgcctactgc
      781 gaccggattt cgatcatgca ccgcgggagg atcatcgagg tgggcaaacc gttcgacatc
      841 gtcgccgccc acggccgcga gaccctcgag gacgccttcg tccacctcat cgccgcacag
      901 gagggcgccg gtgcataaga tccgcttcat cgcccgcaag gagctctacc acatactccg
      961 ggacccgcgc tcgctcatca tcgtcctcgc catgcccatc ctcatgacat tcctctacgg
     1021 ctatgcgatc aacctcgaca tcgagcacat cactctggcg gtgctcgact acgatcggac
     1081 ggcgggctcg cgggatttga tcgaggcctt ctacggctcg agctattttt cgccccccga
     1141 gacgccggtt cggccggagg atccggaggc ggtcctcaag tcggccgagg cccacgcggt
     1201 gctggtgatc cggcccggtt tcgccgaagc cctccggacc gggcagtcct tcgatctcgg
     1261 gctgtacatc gacggttccg acaacaacat ggcggcggcc gtgcagaact acgccgaggc
     1321 gctcctgttg gccgggccgt acgccggccg cgccgacggt cgggctcccg gcgggatcac
     1381 cctctcccgc caggtgctct acaatcccga tctcaagtcg gcccagttct tcgtgccggg
     1441 attgatcgcc ctcatcctcg tgatgatttc ggcgctcctg acctcgatca cgatcgcccg
     1501 cgagaaagag accggaacca tggagcagtt gttgaccgcg ccggtgacgc cggtgcagat
     1561 cctggtcggc aagatcgtgc cgtacatctt catcgccctg ctcgacgccg ccgccatcat
     1621 cgtcatcgcc cgcaccgtat tcggcgtccc ctttgccggc tcgcgactgc tcatgctcgg
     1681 ctacgccctc gtctacatcg gggtgtcgct ctccctgggg atactcatct cgtcgctggc
     1741 caagacgcag caggtcgcca tgatcttcgc cgtcacgacc acgatgctgc cgtcggtgat
     1801 gctgtcgggt ttcatcttcc cgatcaagaa catgccggtc gttctccagg ggctgagcta
     1861 catcatcccg gcccgctact tcgtgcggat catccgcgcg gtcatgcaca agggggcggg
     1921 gatggaggcg ctcgtgctcg aggggctggc gctagccgtg ctcatggtcg tgctcatggc
     1981 cgtcgccgcg agaaaattcc gaatgaaagt ggcgtgatca tggatacccg cgccctccga
     2041 ggactggttc gaaaagagtt catccaggtg ttccgcgacc ggaacatgct cctcatcatc
     2101 ttcgccatgc ccgtcgtcca gctcatcatt ttcggctacg ttgccaacac cgaggtgagg
     2161 gaggtcgccc tcgacgtcta cgacttcgac cgcacggccg tgtcccgcca gtacgccacg
     2221 gcgctggggg cggggaacta ctttaccgtg cagtcgccgg aggcgcccct gctcgaggtt
     2281 gaggagcgtt tccgccggag cgacgccgag atggcgctgg tgattccgcc caatttcacc
     2341 cgcgatctca cctcgggcgg ccgtcccgtc gtggggattg tcgccgacgg cgccaactcc
     2401 aacaccgccg ccatcggcat gggctacctc gcccgcattt ccgcccagta tgcccgcggg
     2461 gttacggagg tgtccgcgcc ggtgcggttg cgcttccagg tcctgtacaa tcccgaaatg
     2521 gagtcggtct acttcatggt ccccggcatc gtcgcggccc ttctgactat ggtcaccgtc
     2581 atgctcacct ccatggctgt cgtccgcgag cgggagcggg gaactctcga gcagctactg
     2641 gtcacgccga tttcgacggt caccctgctt ctgggcaaac tcctgccctt cgccgtgctg
     2701 gggatcgtgg agatgattct ggctctgacg atcggggtgc tttggttcgg gatacctttc
     2761 gccgggtcgg tgccgctgct gtttgcgctg tcggggctct acttgttttc gacgctgggg
     2821 atcggtttgt tcttttcgac gatcacctcg acccagcagc aggctatgtt tttcgcctgg
     2881 tttttctcgg ttttcgcgct tttgaccagc ggcctgttca ccccgatttc caatatgccg
     2941 caatcggttc agtacctgac gtacgtgaac ccgatgcgct acttcatgga catcgcgcgc
     3001 gggatcatga tgcgcggcgc gggcgtgggc gaactggccg gcgacatctg gccgctggct
     3061 gtcttcggcg ccgtcgtcct gtctgtctcc atcatgcgtt tctccaagcg caccgcctga
     3121 ccggggccgg ggcgcccccg gggcggtcgg ctgcattctc ctgctggaca cgtcgcctcc
     3181 gccgcgtata ttaccggagg agccccgcaa ccggctcccg ccgatgaaag gaccggcagc
     3241 catgccgata tacgaattca agtgcggccg ctgcgccgcg acgtttgagg aactggtgag
     3301 cagcggcacc gacgcggtgc cgtgcccgaa gtgcggttcg gccgatgttt accggctgat
     3361 ctcgctggtt tcgtcgaagg ggatgtcggc cggaagttgc gccggctgcg ctccctctcc
     3421 cgccaagtgc cgcgggtgct ccggcggcca ctagagccgt tcgggcagtt ctcccacagc
     3481 gcgaaacgac tgtttcacgc agtcgttcag cccgatcccg tagtaggcat tgccggtcac
     3541 ggcgatattg cccagccgcg cgagttcggc ctcaatgagc gccagccgtg caaggtgccc
     3601 gatcgtgaac tgggggatgc cgcgctccca gcggtagatg cggatcactt ccggcccgcc
     3661 gcggagggcc atgaccgcgc tgaggtcccg gtgcacggtc tcctgcaatt cctcgtcggt
     3721 gaggttcatc acctcccggt cgccgtcccc gccgaccatc gtgcggaact gcacttttcc
     3781 cgccggagcg cggtcggcga aaatcgacga ggtccagatc gagccgagga tccggcgtcc
     3841 ctcgacgcgc gggacgagga acccgaaccc gtccaccgcg cgccccaccg ccgcgccgtc
     3901 gtagccggag cagacgaccg cgatcggggc gtactcgatg gccgcgaggg cctcgctcag
     3961 ctttgccgag agcggccgca cgagcgcggc cgcctcatag gagggcgtcg caaggatgac
     4021 ccgcggcgcc cgcacctccc ggccgtcggc gagccccacc cggtagcccc ggccgtcccg
     4081 ctcgatgcac acggcgccgg cgcccgtggc gatgtcgtcg ccgtagcgtt cgcgggcgcg
     4141 cgcgatcacg gcgtcgagac cgccgcgaaa cgaggtgagc cagccgccgg gaccggccgg
     4201 gccgcccgcg gcgcgcccgg accgcttcgt cgcccgcatc ttcgccacca tcgcccgcag
     4261 gagcgaaccg tactccgcct ccatttccgc catgaggcgg aaggtcgccc gcagactcag
     4321 ccggtcggcc atccccccgt acacgcccga gaccatcggc tgcacgaggt agtcggccgc
     4381 ttcccggccg atccggcggc gggcgaaatc gtagaccgac tcgtcgattc cctccggccg
     4441 cttgcgcccg aacggttcca tggccacgcg cagccggccc ctcaggctga ggatgtcgga
     4501 ccgcagaaac gccggggggg acatcggcac ctcgcggagc cgcccgcccc ggaggatgaa
     4561 ccgcttctgc accgccgcgc tggcccgctc cagccggtct tccaacccca gctcccccgc
     4621 cagccggagc gtgagcggct cccggtcaag gaaaccgttg gggccccagt cgaagctgta
     4681 ccccgcgacc cggtcggtcc cgactgtccc gcccagccgc gcctccttct cgtagagcgc
     4741 ggctgccagc gccggccgca gggtgcgcgc gaagtgcagc gcggccaggc cggagatgcc
     4801 cccgccgatg atcgcgagat ccttagagat gctgctcgac attgcgccgc gcgtcgatga
     4861 tgcgcgcgag cgtccgcgcc aaccggggat cgtcgttgaa catcggcatc cggcggatcc
     4921 gctcgcccgc gttgaggagc tgtttcagtt cgatatctat ttcgtagagc gtctcgatgt
     4981 ggtcgcagac aaagctgatc ggcacgacaa acagccgccg ccccgcgccc agcagccggc
     5041 gcgcctcggc cactgtgtcc ggccccaccc acgccaccgg gcccgtccgg ctctgaaacg
     5101 acaggaaata ttcgcggtcg cccgccgcca gcgaggcggt ggctctcacc tggtccacgt
     5161 acgggtcgcc gtcgtccaca aacgactgcg gaatggcgtg cgccgaaaac aggatcgtct
     5221 cgtcctcgcg catgtggttg ccgatatact cccgcagcag gaagaggtaa ccctggttat
     5281 tgtggaagtc gtcgatgaac accgggtgca cgtcgtcgta ccgttcgagg gcccggcggg
     5341 cctcggcgaa cgacgatcca gtggtggcgc ggcagtactg cgggtacatc gggaggaaaa
     5401 acatcgtccg gcagccgtcc tcgtacgcct ggcgcacggc ctcgccggtc atcggcggcg
     5461 cgtagcgcat cccgatatag cagcgcagcg ggcgggtcac cgccccttcg gcaaacgacc
     5521 gctcgaggtg cgccttctgc gcctcggtcc agtgcagcag gggagagccg ccgccgatca
     5581 aatcgtagtg ccgcttcacc ttctccgccc gccgggccgc gatcagtttc gccagcggtt
     5641 tctgcagcag cggcccgccc ggcaggcgga tgatgcggcg gtcggagaag atgttgtaca
     5701 ggtaggccgg cacttcggcc gtcgtccgcg ggccgcccat ggccagcagg atcacgcagg
     5761 caggtgtttc cgaaaacgcc attcaaatcg tccgtgaaaa ttgcggccgt tcgcccgacg
     5821 gctgggcgcc gaggtaggcg tcgaacgtca tcgcgatgtt gcgcacgaaa gtgcgtccga
     5881 gcggcgtcac gcgcagctcg ccctcgcctt cttccaccag accgtcgtcg aaaaactccc
     5941 gcagccggga gtgctccgcg ccgaaatact cggcatagtc cgcgccgaac cggtcgcgca
     6001 gcgcccggta gccgagccgg aaattgcaca tgagaccggc gatgacatac tgccggatga
     6061 gatcgtcgcg cgagagcacc aggccccggt agaccgcgaa tgcctccccg tccatggccg
     6121 cgtggtaggc cgccagtgtg ctgtggttct ggaagaagct gtcgttgagg tagccgatcg
     6181 aggacatccc caggccgatc atgtcgggcg aggttttgac cgtgtacccc atgaaattgc
     6241 ggtgcagctt gccgtcggcc tgcgcccgcg ccagctcgtc ctcggggagc gcgaaatggt
     6301 ccatcccgat ctggcggtag ccggcccccg tgaacatctc caccgccgtc gcaaagaggc
     6361 cgtacttgac ctcggtgtcg gggaggtcat ccggcttgat cgcgcgctgg ttggccatgc
     6421 gctgcggaag gtaggcgaag ctgtacaccg cgacccggtc gggccggagc gagagagcca
     6481 ggcggatcgt ctcggcaaac gacgccaccg tctgttttgg aaggccgtaa atgaggtcga
     6541 aattcagccc cttgaactcg agcgcacggc agtgcgcgac cgtctcggcc accaggcggt
     6601 acggctgtat gcgccccacc gccgtctgca ccgcctcgtt gaaatcctgc acgccgagcg
     6661 acacccggtt gaacccctgc ccggcgagaa aactcaactg ctccggcgtc gtgacccgcg
     6721 gatcgatctc gatcgacttc tcgcagtcgt cggcgtaccg gaacacgctc cccagcgagc
     6781 cgagcacgtt ccccagctcg tcaaccgtca gataggtcgg cgtgccgccg ccgaagtgga
     6841 gctggttgac cgtccgccgc ccgccgaggt gggccccgac ccgctcggtc tcccggccaa
     6901 gcgcagcgag gtaagcgcct accgcctcgc gttcgttcag aatgcaggtg ttgcagccgc
     6961 agtaaaaaca ccgcttgcga cagaacggga tgtggcagta gaccgacaac ggcgtgtcgg
     7021 tctgcgcgcc cgccgcctcc agcgccttca catacgcctc ccggccgacc gcctccgacc
     7081 actccggcgc cgtcgggtag ctcgtgtacc tcgggccggg gcggtcgtac ttccggagca
     7141 gttcaatcgg gacatgggtg gcgctgtggg cgtggtcagc catcgtcgtt acctgtagtc
     7201 gtgcaccgtc tgcacgagcg tctccaccgc ctcgaccggg gtctccggcc ggataccgtg
     7261 gccgagattg aaaatgagcc gccgcgggtc ttcgattttg tccaggatcg accggacgcg
     7321 ggcgatgatc gtctcgcgcg tcccgaacag cacggccggt tccaggttcc cctgcaccgc
     7381 tttgccgggg agggccgccg ccgccgcctg caggtccatg cggtagtcga caccgaccac
     7441 ctcgcagtcg agcgcgctca ccatgtcgat gtagggggcg aggttgttga caaacagtat
     7501 gcggggcgcc gtgcgcggcg ccagccgggt gaaaatctcc tctgtccagc ggagcgacca
     7561 gtcgcggtag tcctcgcgcg agagaatccc cccccagctg tcgaacaact ggaccgcgtc
     7621 gcagccggcc gccagttggg cctcgaggta gcggacggtc acgtccgtga ccagcgccat
     7681 caggcgctcc gccgcctccg ggtgccggtg aatgaactgc ttgacggtct cgaaatgcct
     7741 ggagcctttc ccctcgatga ggtagcaggc gattgtgaag ggcgacccgg caaaacctat
     7801 caggggcgtt tcgggcaaga gccgcttaat ctcgcggatg ccgtcgcaga cgaaggccag
     7861 ctcggccgcc acgtcgaaat cgtgcagccg ctccgcgtcc tcgggtcgcg cgatcggccg
     7921 ggccagttcc ggcccgccct cggcgaacga aactgcggcc cccatcgggt ccagcacgat
     7981 gaggatgtcg gagaacagta tggcggcgtc gagcccgaac cgccggaccg gctggcggac
     8041 aacctcggcg atcagcttcg ggtcgcggca gagctgctcg aacgtcgcct gccgccgcac
     8101 cgcccggtag tcggggaggt agcgccccgc ctggcgcatg atccagaccg gtatccggcc
     8161 gtcgttgcgg ccgctgcagg cttggagaaa cgggctgttc gctaggtcca cgtgtgcgct
     8221 tcctctttga tcagttcggc cagcgtctcc gccgccccgt cgagcagggc gtcgtcgtgg
     8281 gctgcggaga tgaagcaaac ctcgtaaccc gagggcggca ggtagagacc ggcgtcgaga
     8341 acttttccat ggaaccggtt gtagtgggcg atcccgtcgg ggtcgatcgc ccgggcggcg
     8401 cggggaatgt gctcctggaa cacgatccag aagatcgagg cgaccccgac cacgttcacc
     8461 cggttgcccc gcacgcgcgc cgtgatcgcg ctcacgaagc ggcggttgcg ggcctcgagg
     8521 tcggcgtaga tgcgcccgtc ggccagcttc tccagcgtcg ccagcccggc cgtcatcgcc
     8581 accggattcc ccgagagcgt ccccgcctgg tacaccgggc cgtcgggcga caggaggtcc
     8641 atgatctcgg cccgcccgcc gaacgccccg accggcatcc ccccgccgat caccttgccg
     8701 aaggtcacca ggtccgggtg gatgccgtag tactgcaggg cgcctcccag gccgaggcgg
     8761 aacccggtga tcacttcgtc gagcaccagc agcgccccgt gtttttccgt cagcgcgcgg
     8821 agcaggcgca tgtactcgtg gcgctggatg agcaggccat tgttggccgg gatcccttc
//
LOCUS       DAOPOE010000114         8524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_9285_length_8524_cov_5.191168, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000114 DAOPOE010000000
VERSION     DAOPOE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_8524_cov_5.191168"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PKM94_12090"
     CDS             complement(<1..1528)
                     /locus_tag="PKM94_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD67414.1"
                     /translation="MKELAAYTLSLFAGLIMLGLNTGAAAAQPSLAPGLAELIDRGAP
                     PDSIVHVVVFLETERTAAASKASAAMLARGDQIRQVTSALKQARGRGDRAVAALLRQH
                     ARSTVARYWIAPAYAAAVPAAVLPELAAIDGVSLVVEDAPVSLIAPVEEKAPTAGLAA
                     TVAQPLVQLGVPALWQKGLTGRGRLICSFDTGVKYDHAALLPKWRGAHVPLSAAWFSK
                     VAPTSLPADAVGHGTHTMGIMVGSDGADTIGVAPDAEWITAGVIDQGRPLSTTIADIL
                     EAFQWALNPDGDTMTTDDVPDVILNSWGIPKGLFTPCDRTFAAAIENVEAAGVVTVFA
                     AGNEGPNAMTLRNPADMVLSPLSAFAVGAVDANNAVASFSSRGPASCDQVSVKPELVA
                     PGVSIRSSYKDGGYKVLTGTSMAAPFVAGLVALVRQYNPNVSAADIKSAFIAAARDLG
                     PLGEDNSYGYGIVDAARLLQYIPDPVAPDFSISRVAVSGDGLAVPGERFDLAITLRNP
                     A"
     gene            1806..3983
                     /locus_tag="PKM94_12095"
     CDS             1806..3983
                     /locus_tag="PKM94_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67415.1"
                     /translation="MAPHRQQGGFPASLGLILLVAAGLRVCYLAMYAGLPEWTMLTVD
                     NCFHHHWAQDIAAGNILGATTYFRAPFYVFVLAALYAVFGVSLWTARVFGLAVGLASV
                     ALTYVLGRRAVSSRVGLLAAGLHAVWPTALYFESELLLDPLFLLLLQASVWRLLVWRE
                     THRARDAFWLGLALGLAAITRPTALLLLVVVAGVALCARRGLAPLLRTALAVGLGLAI
                     AIGPIFIRNLAVAGDPVLIASQGGINLYIGNNPEADGVSAVLPEPLGFNWQIRDVVHI
                     AEQAAGRPLAPGEVSDYWAQRARTWIAAHPLSFLRLYAEKLYWNISHREISNNRALGP
                     FFALIPLLRYNPLSFGLLFALALAGLAAALRTNARLRLIALILLLYVAASALFFFNSR
                     FRLPVLPLYFILAATGVFGLAEAVRRRRGLVLPAAALTVGAGLSFLPLVSLPSGVSPQ
                     AIMSAANYAYGQGDFRRALELSAEAAAVDSTYPEVNLNLGNAWLRLGDAAAARAAWER
                     EARFHPGRPKALTNLASLALLEGRFADAQAEGQRALALRPYDYDANLVWLRAVFAADT
                     TAPEQLVAEVQAAAARTGGDVYLLNEAGILLTQRGLAETARATLLAALSAPPPPIEMD
                     DAAFAPNFRHSRERMREQHAQAAYQLGYLAALASRFGEAVEFSRRAVELDPGLAEAWV
                     NLVGACAAAGRIDEARTTLREALARFPNRPELRELDRRLAPRQ"
     gene            complement(4044..6434)
                     /locus_tag="PKM94_12100"
     CDS             complement(4044..6434)
                     /locus_tag="PKM94_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67416.1"
                     /translation="MRALLTTLVLAGVGGGAALLVGGCTDTLDGERFANQKPIVHFAN
                     IPPEGQTFSRNPVVYWYGSDADGLIDYYRYHVATEAEVGFDSPENYIQRVSDAAWTHV
                     DVDPAASDPQTTHQIPLAADSANPVRSFVVQYVFLQAFDMEGLGSDIVYRRFARNDNP
                     PNTGINDISTKIPFVNAPRPGGIVTGIALDWMANDSVDYPSDPPRFEFQWRLYGPYTN
                     DEMIAMEAQFVQTVFQSVDGFVYRQGEYFKVCDTTFVSGNPDPVIDCDSFLVGTDEID
                     VRLGRTFKNYFDFEDADFVNNPVYYKVVDSSCYRDPECLKSLPDSLDETACPCLDTWV
                     PALSQSNSREWAGRDTLYNVFRHTPTDSTVERQFLFWIRSRDDAKVPDPTPAWKAFPV
                     VEPKFERDVLVFDMAASAQPIAVRKADGYLPDNTTDPSRPRSYLFWRDMLLNWNPDLN
                     FNEYDYIYQGRSYNNTNYTNGIPLVKLLQYKVVVLYNDHIVAPPITKEGYMKPVITGL
                     DAGVNAWSICRSVAGGDGNKSHMPPGDPQLIFNQVSNKWAMAYKRYFAVNSGLYTGWF
                     QHANRLRDPRPNAPWALPAFDVQDCIGAYSLQPAQWPDIEYDSALVHDLYEWGSLVPD
                     TSTPDPSDRIQYYYNKKLPYLPEINWCQARYGAEIMYLYKSYYRSRGVAHPLGVYEGI
                     SGSFNFDFEGAPCGHRYSTGLYRTVHMNLTPFALKRGPNPTSGPVAQLFRAVMDYLYD
                     PNIQQPVAKLRYHDADPTAAADLSVERDRYWKRCDQEAYEKGLDLSAHALANLHRR"
     gene            complement(6480..7454)
                     /locus_tag="PKM94_12105"
     CDS             complement(6480..7454)
                     /locus_tag="PKM94_12105"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOD67417.1"
                     /translation="MLRHEHTRALGRTRGIALSGAVLAAVLLLVSPGDVRAQAKVGTT
                     GAQFLEIGVSARAMGMAEAFTALANDVSAVYYNPAGLVYLYGPEGQLTYVRMPADVNY
                     GYAAIGLPLESIGGVLGISAYHLNSGEIDEIDYYAIPTGRTFWWKDFSLAVSYGRYLT
                     DRFSVGFTVKYIGEFVHDYSANGWAADVGTVYNTGFRNFKIAMAITNFGPDLNMIRDD
                     FPLPINFRFGGTIDALQTDNYVLSVAAEGCHPSDNLEKYNSGAELTIRDMFILRAGGR
                     FNYDVDGLTFGGGLRLPFGEESEIRVDYAYQDFGVMDEVHRFTMSFAF"
     gene            complement(7499..>8524)
                     /locus_tag="PKM94_12110"
     CDS             complement(7499..>8524)
                     /locus_tag="PKM94_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD67418.1"
                     /translation="DLIPVAENDDIGSGVILGDRQKLSPRIGFSYPISDKAKVHFNYG
                     HFYQMPDLDAMYARNTTSIQQDAVIGNYNLDYMKTVQYSFGVKYAMSEYYNIDVSGYF
                     KDEFDKINSAFVVIDNFNRQQYRNKDYGRSRGFEVELEKRGGGYVNGMIAYTYGFAYG
                     KASEASRTYQTSFELSREPLDEAPLDNDIRHSLKGNIQVTVPNTVKPRLFGMPIPNGW
                     SLNLQSIIESGRPFTPDRSYPNVATVGGEDIQRNSLRMPSTVLFDVRFTKDFQFAGVD
                     LSYILWIENIFDTRNVAEDGIYTNTGRADTRQNQSGIIRGGTEYDANPYNWDYGRQVR
                     MGIQINL"
BASE COUNT         1354 a         2835 c         2764 g         1571 t
ORIGIN      
        1 ccgccgggtt gcgcagcgtg atcgccaggt cgaagcgctc gcccggcacc gccagcccgt
       61 cgcccgaaac cgccacccgg ctgatggaga aatcgggcgc caccggatcc gggatgtact
      121 ggagcagccg cgcggcatcg acgatcccgt agccgtagct gttgtcctct ccgagcggcc
      181 ccaggtcgcg ggcggccgcg ataaaggcgc tcttgatgtc ggcggccgac acgttgggat
      241 tatactggcg gacgagcgcc accagtccgg ccacgaacgg cgcggccatc gaggtgcccg
      301 tgagcacctt gtacccgccg tctttgtacg acgaccggat actcacgccc ggggcgacca
      361 gctccggctt caccgacacc tggtcgcacg atgccggacc ccggctggag aagctcgcga
      421 ccgcgttgtt ggcatcgacg gcgccgaccg cgaaggcgct cagcggactg agcaccatgt
      481 cggccgggtt gcgcagcgtc atggcgttcg gcccttcgtt gccggccgca aacaccgtca
      541 ccacgccggc cgcctccacg ttctcgatcg ccgcggcaaa cgtccggtcg cacggcgtga
      601 agagaccttt cgggatcccc cagctgttca ggatcacgtc gggcacatcg tcggtcgtca
      661 tcgtgtcccc gtccggattg agcgcccact ggaaagcttc gagaatgtcg gcgatggtgg
      721 tcgagagcgg ccgtccctgg tcgatcacgc cggccgtgat ccactccgcg tccggggcca
      781 cgccgatcgt gtccgccccg tcggagccga ccatgatgcc catcgtgtgg gtaccgtggc
      841 cgaccgcgtc cgccggcaga cttgtcggcg ccaccttgga gaaccaggcc gcgctcagcg
      901 gaacgtgcgc gccccgccac ttcggcagga gcgccgcgtg atcgtacttc actcccgtat
      961 cgaacgagca gatgagccgc ccgcgccccg tcaacccctt ctgccacagt gcggggactc
     1021 cgagctgcac aagcggctgc gcaaccgtcg ccgccagacc ggccgtcgga gccttttcct
     1081 cgaccggcgc aatcaggctc accggcgcat cttcgaccac gaggctcacg ccgtcgatcg
     1141 ccgccagttc cgggaggacc gccgccggca ccgctgccgc gtaggccggt gcaatccagt
     1201 aacgggcgac ggtcgagcgg gcgtgctgcc ggagcagcgc ggccaccgcc cggtcccccc
     1261 gcccccgcgc ctgcttcagc gccgatgtga cctgccggat ttgatctccc cgggcaagca
     1321 tcgcggccga cgctttcgac gcggcggcgg tgcgttccgt ctcgaggaag acgaccacgt
     1381 gcacgatgct gtccggcggc gctccgcggt cgatcagttc ggcgagcccc ggcgccagcg
     1441 acggctgggc ggcggccgcc ccggtgttca gccccagcat gatcagaccc gcgaacagcg
     1501 agagcgtgta ggctgcaagt tccttcatgg gttctcctcc ggcaaagtcc ggggaccctt
     1561 agagcaacgg ttatgccgcc ccccggctta tgagggggcc gcaaccggct gcaccgactc
     1621 accctcaggg cgagctaatc gctattaccg caacgacttt cgggggaggc gctggggtcg
     1681 cgcgagcggg gcgagcggtt gaccacttgc acaggtgaaa ggccggtgca gtccgatgca
     1741 caggcgggtg cacactcggc aactttttac aggctgattc cagcggcggg ttctatattg
     1801 tcccgatggc tccacaccgg caacagggcg gttttcccgc ctcgctcggg ctgattctgc
     1861 tggtcgccgc cgggctgcgg gtctgctacc tggccatgta cgccgggctg cctgagtgga
     1921 ccatgctcac ggtcgacaac tgcttccacc accactgggc gcaggatatc gcggccggga
     1981 atatcctcgg cgccaccaca tacttccgcg ccccgttcta tgttttcgtg ttggccgccc
     2041 tgtacgccgt cttcggtgtt tcgctctgga cggcccgcgt gttcggtctg gccgtgggcc
     2101 tggcatcggt ggcgctgacg tacgtgctcg gtcggcgggc ggtgtcgtcg cgcgtcggtc
     2161 tcttggcggc cggtctccac gccgtgtggc cgaccgcgct ctacttcgag tccgaactgc
     2221 tgctcgaccc gctgtttttg ctgctactgc aggcttcggt atggcggctg ctcgtgtggc
     2281 gggaaacgca ccgggcgcgg gatgcgttct ggctcggact ggcgcttggt ctggcggcga
     2341 tcacgcgacc gaccgcgctg ttgctcctgg tcgtggtcgc gggggtcgcc ctgtgcgccc
     2401 ggcgcggcct ggcgccgctc ctgcgcacag cgctcgcggt cggcctcggc ctggcgattg
     2461 cgatcggacc gatcttcatc cgcaacctcg cggtcgccgg cgatccggtg ctgatcgcct
     2521 cgcagggagg catcaacctg tacatcggca acaaccctga ggccgacggc gtctccgcgg
     2581 tcctgcccga accgctgggt ttcaactggc agattcggga tgtcgtgcat atcgcagagc
     2641 aggccgccgg ccgtccgctc gcacccggcg aagtctccga ctactgggcg cagcgcgccc
     2701 gcacgtggat cgccgcccat cccctctcgt ttctccgcct ttatgcggag aagctctact
     2761 ggaacatctc gcaccgggag atttccaaca accgcgcgct cgggccgttc ttcgcgctca
     2821 tcccgctgct gcgctacaat ccgctgagtt tcggtctgct ctttgcgttg gctctggccg
     2881 gtctggccgc ggcgctgcgg accaacgccc gtcttcgcct gatcgcgctt atcctcctgc
     2941 tgtatgtcgc cgcctcggcg ctgttcttct tcaacagccg cttccgcctg ccggtgctgc
     3001 cgctgtattt catcctggcg gcaaccggtg tgttcgggct ggccgaggct gttcggcggc
     3061 ggcgggggct ggtgctgccg gccgcggctc tgacagttgg cgccgggctg tcgttcctgc
     3121 cactggtgtc gctcccgtcc ggtgtgtctc cccaagcgat catgagcgcc gccaattacg
     3181 cctacggaca gggggacttc cgccgcgcgc tcgaactctc ggccgaagcg gccgcggtcg
     3241 actccaccta ccccgaagtg aacctgaatc tgggcaacgc ctggctgcgt ttgggcgacg
     3301 ccgctgcggc ccgggctgcc tgggagcggg aggcccggtt ccaccctggc cgacccaagg
     3361 ctctgaccaa cctcgcctcg ctggccctgc tcgaggggcg tttcgccgac gcgcaagcgg
     3421 aggggcagcg ggcgctggcg ctccgcccct acgactacga cgccaatctc gtttggctgc
     3481 gggccgtgtt cgcggcggat acgacagcgc ctgagcagct tgtcgccgag gttcaggccg
     3541 ctgcggcgcg cacgggcggc gacgtctacc tgctcaacga ggccggcatt ctgctcacgc
     3601 agcgcgggct ggccgagacg gcgcgcgcga cgctcctggc ggccctgtcc gcgccgccgc
     3661 cgccgatcga gatggacgat gccgctttcg ctcccaattt ccgtcacagc cgcgagcgca
     3721 tgcgcgagca gcacgcccag gcggcctacc agctcggcta tctcgccgct ctcgctagcc
     3781 gcttcgggga ggctgtcgaa ttctcgcgcc gggcggtgga actcgacccc ggcctggctg
     3841 aggcctgggt gaacctagtc ggcgcctgcg ccgcagcggg gcgcatcgac gaggcgcgaa
     3901 ccaccctccg cgaggcgctc gcccgcttcc ccaaccgccc ggaactccgg gagctcgacc
     3961 gccgcctggc gccccggcaa taaacaaaaa gagaccggag ccgacggctc cggtctcggt
     4021 aacggtcaaa taacggcggc cggtcagcgc cggtgcagat tggccagcgc gtgggcgctg
     4081 agatccagac ccttctcgta cgcttcctgg tcgcagcgct tccagtagcg atcccgctcg
     4141 acggagagat ccgccgccgc cgtcgggtcc gcatcatgat accgcagctt cgcgaccggc
     4201 tgctggatat tcggatcgta gaggtaatcc atgacagccc tgaagagctg ggcgaccgga
     4261 ccggaagtcg ggttcggccc gcgcttgagc gcaaacggcg tcaggttcat gtgaaccgtc
     4321 cggtaaaggc cggtgctgta gcggtggccg cacggcgccc cttcgaaatc gaagttgaag
     4381 gagccggaga tgccctcata cacaccgagc ggatgggcaa cgccccgcga ccggtagtac
     4441 gacttgtaca gatacatgat ctccgccccg taccgcgcct ggcaccagtt gatctccggc
     4501 agatagggaa gcttcttgtt gtagtagtac tggatccggt ccgagggatc cggcgtggag
     4561 gtatccggga ccagcgaccc ccactcgtag agatcgtgca caagcgcgga gtcatactcg
     4621 atatcgggcc attgcgccgg ctgcaggctg taggcgccga tgcaatcctg aacatcgaat
     4681 gcgggcagag cccatggcgc attggggcgc gggtcccgca gccggttggc gtgctggaac
     4741 cacccggtgt acaagccgct gttcaccgca aagtaccgct tgtatgccat ggcccacttg
     4801 ttgctgacct ggttgaagat caactgcggg tcgcctgggg gcatgtggct cttgttccca
     4861 tcgccgcccg cgaccgagcg gcaaatcgac caggcattca cgccggcatc cagacccgtg
     4921 atgaccggct tcatgtagcc ctctttcgtg atcggcggcg ccacgatgtg atcgttgtac
     4981 aacaccacga ccttgtactg cagcagcttc accagcggga ttccattggt gtagttggtg
     5041 ttgttgtacg accggccctg atagatgtag tcgtactcgt tgaaattcaa atccgggttc
     5101 cagttcagga gcatgtcgcg ccagaacagg taactgcgcg gacggctcgg gtcggtggtg
     5161 ttgtccggta ggtatccgtc ggccttgcgg accgcgatcg gctgggcgga agccgccatg
     5221 tcaaatacca gcacgtcccg ctcgaacttc ggttcgacca ccgggaacgc cttccaggcc
     5281 ggcgtcgggt cgggcacctt ggcgtcgtcg cgggagcgga tccagaagag gaattgccgc
     5341 tccaccgtcg agtcggtcgg agtgtggcgg aacacgttgt agagcgtgtc gcgacccgcc
     5401 cactcgcgcg aattggactg gctgagtgcg ggcacccacg tgtcgaggca cgggcaggcc
     5461 gtctcatcca gcgaatccgg cagactcttc agacactccg gatcgcggta gcacgagctg
     5521 tccaccacct tgtagtagac cgggttgttc acgaagtcgg cgtcttcaaa atcgaagtaa
     5581 ttcttgaagg tccgtccgag gcgaacgtcg atctcatccg taccgaccag gaacgaatca
     5641 caatcgatga ccgggtcggg attgccggac acgaaggtcg tgtcgcagac tttgaaatac
     5701 tcgccctggc ggtacacgaa accgtccacg ctctggaaca cggtctgcac gaactgggcc
     5761 tccatcgcga tcatctcgtc gttcgtgtac ggtccgtaga ggcgccactg gaactcgaag
     5821 cgcggggggt cgctcgggta gtcgacggag tcgttagcca tccagtccag cgcaatgccg
     5881 gtcacgatgc cgcccgggcg cggggcgttg acgaacggaa tcttggtgct gatgtcgttg
     5941 atcccggtgt tcggcggatt gtcgttgcgc gcgaaacggc ggtagacgat gtcggagccg
     6001 aggccctcca tgtcgaacgc ctggaggaac acgtactgga ccacgaacga gcgcaccggg
     6061 ttggcgctgt cggccgccag cgggatctgg tgcgtcgtct ggggatccga ggccgccggg
     6121 tcgacgtcca cgtgcgtcca cgcggcatct gagacccgtt ggatgtagtt ctcggggctg
     6181 tcgaaaccga cttccgcctc ggtcgcgacg tggtagcggt agtagtcgat gagcccgtcg
     6241 gcgtccgacc cataccagta gaccacgggg ttccgcgaga aggtctggcc ctccggcggg
     6301 atgttggcga agtggacgat gggcttctga ttcgcgaagc gctcaccgtc cagcgtatcc
     6361 gtgcagccgc ccaccagaag cgccgccccg ccccccactc cggccagcac cagcgttgtc
     6421 aggagtgcac gcatcgttcg atatctcata actgtcttca tctgctgcct tgcctcccgc
     6481 taaaacgcga aactcatggt gaatcggtgc acttcgtcca tcacgccgaa gtcctggtag
     6541 gcgtagtcga cgcggatttc ggattcttcg ccgaacggca ggcgcagccc gccgccgaac
     6601 gtgagcccgt ccacgtcata gttgaaccgc ccgccggccc gcaggatgaa catgtcgcgg
     6661 atggtcagct cggcgccgct gttgtacttc tcgaggttgt ccgacgggtg gcagccctcg
     6721 gccgccaccg acagcacgta gttgtcggtc tgcagcgcat cgatcgtccc gccgaaccgg
     6781 aagttgatgg gcaacgggaa gtcgtcccgg atcatgttga gatcggggcc gaagttggtg
     6841 atggccatgg cgatcttgaa attccggaaa ccggtgttgt acaccgtgcc gacgtcggcc
     6901 gcccacccgt tggccgagta gtcgtgaacg aactcaccga tgtacttcac ggtgaaaccg
     6961 accgagaacc ggtccgtcag gtagcggcca tagctcacgg cgagcgaaaa gtccttccac
     7021 cagaacgtcc gcccggtggg gatcgcgtag tagtcgatct cgtcgatctc gccggagttg
     7081 aggtgatagg cgctgattcc cagcaccccg ccgatcgatt cgagcggcag accgatggcc
     7141 gcgtagccgt agttgacgtc ggcgggcatc cgcacgtacg tcaactgccc ctccggcccg
     7201 tacaggtaga ccaggccggc ggggttgtag tagacggccg agacgtcgtt ggccagcgcc
     7261 gtgaacgcct ccgccatccc catggcgcgc gccgagacgc cgatctcaag gaactgggcg
     7321 ccggtcgtgc ccactttcgc ctgggcccgt acgtcaccgg gagagaccag cagcaggacc
     7381 gccgccagga ccgcccccga gagggctatc ccccgggtcc ggccaagcgc gcgtgtatgt
     7441 tcgtgtctta gcatggcatc ctctttattt gcagcaacaa cttgcctcat cccatgactt
     7501 acaggttgat ctgaattccc atccggacct gccggccgta gtcccagttg tacgggttgg
     7561 catcgtactc ggtgccgccg cggatgatcc cgctctggtt ctggcgggtg tccgcgcggc
     7621 ccgtgttcgt ataaatgccg tcctcggcca cgttccgggt gtcgaaaata ttctcgatcc
     7681 acagaatgta gctcaggtca acccccgcaa actggaagtc cttggtgaac cgcacgtcga
     7741 aaagcaccgt cgagggcatg cggagcgaat tccgctgaat gtcctcgccg cccacagtcg
     7801 ccacgttcgg gtaggagcgg tccggcgtga aggggcgacc gctctcaatg atcgactgga
     7861 gattgagcga ccagccgttc ggaatcggca tcccgaacag ccgcggcttc accgtgttgg
     7921 gcacggtcac ctggatgttc cccttgagcg agtgccggat gtcgttgtcc agcggcgcct
     7981 cgtcgagcgg ctcgcgcgac agctcgaaac tcgtctggta ggttcgggag gcctccgacg
     8041 ccttgccgta ggcaaagccg taggtgtagg cgatcatccc gttgacataa ccgccgcccc
     8101 gcttctccag ctcgacctcg aacccgcggc tgcggccgta gtccttgttc cggtactgct
     8161 ggcggttgaa gttgtcgatg acgacgaacg cgctgttgat cttgtcgaac tcgtctttga
     8221 agtagccgga gacgtcgatg ttgtagtact cgctcatggc gtacttgacg ccgaacgagt
     8281 actgcacggt cttcatgtag tcgaggttgt agttgccgat gaccgcgtcc tgctggatcg
     8341 aggtcgtgtt gcgcgcgtac atcgcgtcga ggtcgggcat ctggtagaag tgcccgtagt
     8401 tgaagtggac cttcgccttg tccgagatcg ggtaggagaa cccgatgcgc ggcgagagtt
     8461 tctgccggtc gcccaggatg acgcccgatc cgatgtcgtc gttctcggcc accgggatca
     8521 ggtc
//
LOCUS       DAOPOE010000115         8395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_9466_length_8395_cov_6.187650, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000115 DAOPOE010000000
VERSION     DAOPOE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_8395_cov_6.187650"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKM94_12115"
     CDS             complement(<1..419)
                     /locus_tag="PKM94_12115"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD67419.1"
                     /translation="MNKPTAFITGIAGFAGSWLAEELLAHGYRVTGGVYADEPLDNLA
                     GCRGQLGLVPFDVADADQAARTIGRIRPEYIFHLAALASVSQSLQQERLTLRVNVEGT
                     LNMLEAARRVRRLRKFVWAGSCDAYGVVRDKTLTEKDP"
     gene            complement(423..1388)
                     /gene="gmd"
                     /locus_tag="PKM94_12120"
     CDS             complement(423..1388)
                     /gene="gmd"
                     /locus_tag="PKM94_12120"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD67420.1"
                     /translation="MKALITGITGQDGSYLAELLLDKGYEVIGMVRRSSTESFERINH
                     IKDRIRLAQADLLDQLSIISIIAEERPNEVYNLAAQSFVPTSWDQPVLTGEFDALGVT
                     KVLEAIRLVDRGIRFYQASSSEMFGKVREVPQKETTPFYPRSPYGVAKVYGHFITVNY
                     RESYGIHASSGILFNHESPRRGLEFVTRKITDGVARIKLGLADKLALGNLEAKRDWGF
                     AGDYVKAMWLMLQQEEASDYVIATGQTHSVRDFVAQAFAHADLDYRDYVSVDPALVRP
                     AEVDLLLGDAGKAHRELKWEPSVTFAGLVSMMVEADLERLRKTLR"
     gene            1556..2539
                     /locus_tag="PKM94_12125"
     CDS             1556..2539
                     /locus_tag="PKM94_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67421.1"
                     /translation="MKHLAALTVLFLAASAAAVEIKPQGVPVTSGDDITADSLLRQGD
                     AEFQARDYAAALRTYLSAVDQARREFNRSVETEALAQTARMCLVQDRKEEGRPYLEQA
                     AAIAVDSDPMGWSRYLGVRGRYQWQDSELAAARETFELMFDYCSVNALFGRAVDAAHM
                     VAIVAASPEDQIAWNRRGIAAAEAADEERWLGPLWNNLGGTYWDNGQFDSALACYLKA
                     RDYHWRFSDETAKLFADYHVGMTYRHLGDYREAQQWLRPVLAWAERLDNREAIGQACE
                     DLGEALLAEGKRAEGTALLRRARDAYRAAGYDRTRPEIWDNITRRLKELGE"
     gene            2543..4615
                     /locus_tag="PKM94_12130"
     CDS             2543..4615
                     /locus_tag="PKM94_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67422.1"
                     /translation="MPAASPRKPHRRPPAASLRPFLLAYAFTVMAFFLLSFVPHGRVW
                     GLNSWAWLPLWGRLLLLIAAAAAPLGALRLSEPSAPAPGRRGAYALAAGLAGIVLFAA
                     FLILRQQTHFLGDGYTLLKWLEEGGAINKMRNFGAQFTLLLADRVLPGGALEAFRIVA
                     WTSGAVAVAAALTASAALFSELPRRLLFACGVLSGGYALLFFGYVENYALFVLMVLLF
                     CWAGLLAVRGRISPWWILLPQAGAVFFHVFGVTLLPAAAYAVLRTTPLGRRVARLPLG
                     TRGLMIAGLAAAGLAAFVWFYTHSYFFRFTFVPILPNRFTIRGYTMFSLSHLVDVANL
                     LLILAPALGLFAVAKFRTGVRRIIAEPSFRFLGLAALGALGAAFIFDPKLGMPRDWDL
                     FSYGGVPLTMAGMLLLLEGPKPLPKPQGLAALAVALALLALVPRVIVQHTPPLAIAQF
                     EAYRDLDPVQNRNGAIFLVDYYERRGDTARAEELRTAWHKAQTDEPLLMQGNAALQSG
                     AFRRALDLYRAALRENPVYWVAWANLGDAYLRMGRRDSALEMYRIADGLNPSSPIILS
                     NLGQAWYLNGQAEKGVELWQRSASLDPEFLPPLRFLVNHYRTSRDRDNYLAVLERIAR
                     LDGAPADNLAALADWYVQSNRFADAGRLYERALAAGLDSAYVSEMMAKYPVLREEFPA
                     WHTTSTAF"
     gene            4588..6615
                     /locus_tag="PKM94_12135"
     CDS             4588..6615
                     /locus_tag="PKM94_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67423.1"
                     /translation="MAHDEHRILTWSLIIFVLTLAAHLVAASLPEMRLWGAAAWARVA
                     PALWAAAVLSALVLLAASFRLPAAGRAPAGRRYVLAAAAAGLATVALFYLLRTKAHFL
                     GDGYTALSLLADDSPLIKTREIGESLAHAWVKRLSGAEGEAGALFSFRFISIAAGALL
                     FGAAAWSAARLFDSAARRALFVAGVMTGGYALLFFGYVENYSLFVASTGIYALVGLLV
                     VRGQVARWWLLIPLAASAACHVLGVTLLPSAVYLLLAPTRAGKRLRTWRVSQRLTVGG
                     LLLLVAGWAVVGVRQSHLFFRYSLVPPFEDAFTIGGYTLFSPAHLADLLNLLVLLLPP
                     VGLLVLVIVSAARSRRLAARDAVFLIVLSLSALGAAFIFDPKLGMPRDWDLFAFAGIA
                     PAVLLYVLTLDPQSPLPGGLRVAAMAVVLSLFALGGRLVAANDPPIQIARFNDYLTVD
                     PERDRNARYLLLTYYKDRGDSAAADRVRAEWRAAFPLRDKLDEAQRLHREEKLPEATA
                     LFEQIVREDPTYADAWAGLGWMMVRYRDWDSAIACLDMSLGLNPYHAAACSNRGLAHL
                     QLGHAQQAARDFERSLSIDPNVPAVMYNLAVAYLRCRRYEDAGRVIARMAAMPDMLAG
                     EHLKLFEWYATYGPLPAAVAQLRFAIDKGADSAYVQYLLQRFPDAAKAFRR"
     gene            6672..>8395
                     /locus_tag="PKM94_12140"
     CDS             6672..>8395
                     /locus_tag="PKM94_12140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD67424.1"
                     /translation="MTSPADHTRTEKLLLEAARIFNSTLEYESLIAEVLRLVNTALNS
                     EGALLFRVDHDRTDIKIRLVRARDNEMFLFHRDFGTGIIGWVARHREPELVYDATADP
                     RVDPEIESRFGSPIRSVVVVPLIGRGHMIGVVEALNKVDGVFTPADLDILVGLANQIA
                     VAIDNAHLYRVARREALEKELLFEIGKKLSSSISQKEVMREILHSVKRAIDFDAGGVF
                     VIEPAKDIIGEVYTEGYDDPGSEERVLVKTGEGLIGHVTATGEPVIVPDVSVDPRYMS
                     ARETTRSEIVVPMLLNNRVVGVLNLESDTPGAYRVDNLSLLQAFAAQAAVSLDRARLH
                     EEILAAKRLEEQLKIAREIQQTFLPEDDPTIPGYDVSGRNVPSGQVGGDYYDFIHIVD
                     YQTGIAIADVSGKGIPAALIMASFRASLIAEIRNNYSIRTIAAKVNNLVYESVKAGSF
                     VTGVYGVLDSRNHIFTFTNCGHNLPFLVRAGGAVEYLREGGQILGVSPNAQYEERPIW
                     IGPGDIILLYTDGVTEVFTETGEEYGLQRLVNLVRDHREKTSQELVDIIHEAVSRWAG
                     PKKLPDDFT"
BASE COUNT         1256 a         3115 c         2517 g         1507 t
ORIGIN      
        1 ggatccttct cggtgagcgt cttgtcccga accactccgt aggcgtcgca ggaacccgcc
       61 cacacgaact tccgcagccg gcggacccgt cgcgccgcct ccagcatgtt gagcgtcccc
      121 tctacgttca cccgcagcgt gaggcgctcc tgctgcagcg actggctcac gctggcgagc
      181 gccgccaggt gaaagatgta ctccgggcgg atccggccga ttgtccgggc cgcctggtcc
      241 gcatccgcga cgtcgaacgg caccagcccc agctgcccgc ggcagcccgc cagattgtcc
      301 agcggttcgt cggcatacac tcccccggtc acccggtacc cgtgggccag cagttcctcc
      361 gccagccagg agcccgcaaa cccggcgatt ccggttatga acgcggtcgg cttgttcatc
      421 gtctatcgaa gagtctttcg cagccgttcc agatcggcct ccaccatcat gctcacgagc
      481 ccggcgaaag tcacgctcgg ctcccacttc agttcccggt gcgccttgcc cgcatccccg
      541 agcagcagat ccacctcggc ggggcgaacc agggccggat ccaccgaaac gtagtcgcgg
      601 taatcgaggt cggcgtgggc gaaagcctgg gcgacaaagt cgcgcaccga gtgggtctga
      661 ccggtggcga tgacgtagtc cgaggcttcc tcctgctgga gcatcagcca catcgccttc
      721 acatagtccc cggcgaatcc ccagtcccgc ttcgcctcca gattccccaa cgccagcttg
      781 tcggccaggc cgagtttgat gcgggccacc ccgtcggtga tcttccgcgt cacgaactcc
      841 agcccgcggc ggggcgactc gtggttgaag aggatgcctg aggaggcgtg aatgccgtaa
      901 ctctcccggt agttcaccgt gatgaagtgt ccgtaaacct tggccacgcc atagggcgag
      961 cgcggataaa acggcgtcgt ctccttctgc ggaacctccc gcaccttgcc gaacatttcc
     1021 gatgacgacg cctggtagaa gcggatgccc cggtccacca gccggatggc ctcgagcact
     1081 ttggtcaccc cgagggcgtc gaattccccc gtcaacaccg gctggtccca gcttgtcggc
     1141 acaaacgatt gggcggcaag attatagact tcgttcgggc gctcctcggc aatgatcgat
     1201 atgatcgaca actggtccag cagatccgcc tgggccagcc ggatccggtc tttgatgtga
     1261 ttgatccgct cgaatgactc ggtcgacgac cggcggacca tcccgatcac ctcgtagcct
     1321 ttgtccagga gcaattcggc gaggtaagat ccgtcctgcc cggtaatgcc tgtaatgagt
     1381 gctttcattc agtctgtccc ttctcgatgg ccggcgcgtc tcggtagtgc gcgtgaatat
     1441 aggggggcgg tccggccgcg tcaaatggaa atgcccggcg cccgatttgc ggtggcggcg
     1501 cccgctccgg tcgattatat tccgtccgcc accggatgca aaccgaggag acggcatgaa
     1561 acacctcgcc gcactgaccg tgctgttcct ggcggcctcc gccgccgctg tcgagatcaa
     1621 accacaagga gtacccgtga cctctggcga tgacatcact gccgactccc tgctccgcca
     1681 gggggacgcc gagtttcagg cccgcgacta tgcggccgcc ctgcgcacct acctgtcggc
     1741 cgtcgaccag gcccgccggg agttcaaccg ctccgtcgag accgaggctc tcgcccagac
     1801 ggcccgcatg tgcctcgtgc aggaccggaa agaggagggg cggccctacc tcgaacaggc
     1861 tgccgccatc gccgtggatt ccgaccccat gggctggtcg cgttacctcg gcgtccgggg
     1921 gcggtaccag tggcaggatt ccgagctcgc ggccgcccgc gaaacattcg aactcatgtt
     1981 tgactactgc tcggtcaacg ccctgttcgg ccgggccgtg gacgccgccc acatggtcgc
     2041 gatcgtcgcc gcgtcgcccg aggaccagat cgcctggaac cggcggggga tcgccgccgc
     2101 cgaggcggcc gatgaagagc gctggctcgg ccccctgtgg aacaacctcg gcggcaccta
     2161 ctgggacaac gggcagttcg acagcgccct ggcctgctat ctcaaagccc gcgactacca
     2221 ctggcgcttc tccgatgaga ccgccaaact cttcgccgac taccacgtcg gcatgaccta
     2281 ccgccacctg ggagactacc gggaggcgca gcagtggctg cgcccggtgc tcgcctgggc
     2341 cgaacgcctt gacaaccgcg aagccatcgg acaggcgtgc gaggacctcg gcgaggccct
     2401 gctcgccgag ggcaagcgcg ccgaaggaac cgcgcttttg cgccgcgccc gcgacgccta
     2461 ccgcgccgcc gggtacgacc gcacccggcc ggaaatctgg gacaacatca ccaggcgcct
     2521 caaggagctg ggggagtaat tcgtgcccgc ggcttcccct cgcaaaccac accgccgtcc
     2581 ccccgccgcc tccctccggc cctttctcct cgcctacgcg ttcaccgtga tggccttctt
     2641 cctcctctcg ttcgtgcccc acgggcgcgt gtggggattg aattcgtggg cctggctgcc
     2701 gctgtgggga cggttgctgc tgctgatcgc ggccgctgcc gcgcctctgg gagcgctccg
     2761 cctttccgaa ccgtctgcgc ccgcgccggg caggcggggt gcatacgcgc tcgccgccgg
     2821 cctcgccggg atcgtcctct tcgccgcgtt cctgattctc cgccagcaaa cccactttct
     2881 cggcgacggc tacacgctgc tgaagtggct cgaggagggg ggcgccatca acaagatgag
     2941 gaacttcggc gcccagttca ctctcctgct ggccgaccgc gtacttccgg gcggcgccct
     3001 cgaggccttc cgcatcgtcg cctggacctc cggcgccgtc gccgtcgccg ccgcgctcac
     3061 cgcgtcggcg gccctcttct ccgaactccc gcggcggctt ctgttcgcgt gcggcgtcct
     3121 ctccggcggg tatgcgctgc tctttttcgg gtacgtcgag aactacgccc tctttgtcct
     3181 catggtcctc ctgttctgct gggccgggct gctggcggtg cggggccgga taagtccctg
     3241 gtggatcctc ctcccgcagg cgggcgcggt gttcttccac gtcttcggcg tgaccctcct
     3301 cccggccgcc gcttacgcgg tgctacggac cactcccctc ggccgccggg ttgcccgtct
     3361 gcctctcggc acgcgcggcc tgatgatcgc cggactggcc gcggccgggc tggccgcctt
     3421 cgtctggttc tacacccaca gctacttctt ccggttcacc ttcgtcccga tcctgccgaa
     3481 ccggttcacc atccgcggtt acaccatgtt ctcgctctcc cacctcgtcg acgtcgccaa
     3541 cctcctgctc atcctggcgc cggcgctggg cctttttgcg gtggccaaat tccgcaccgg
     3601 cgtgcggcgc atcatcgccg agccgtcgtt ccggttcctc gggctcgccg ccctcggcgc
     3661 cctgggagcg gcgttcatct ttgatcccaa actcgggatg ccccgcgact gggatctctt
     3721 ttcgtacggc ggcgtccccc tcaccatggc cgggatgctc ctcctgctgg aggggccgaa
     3781 accgctgccg aaaccccagg gactcgccgc cctcgccgtt gcgctcgccc tgctggcgct
     3841 tgtcccccgg gtgatcgtgc agcacacgcc gccgctggcc attgcccagt tcgaggctta
     3901 tcgcgacctc gacccggtcc agaaccgcaa cggcgccatc ttcctcgtcg actactacga
     3961 acggcgcggc gacaccgccc gggcggaaga attgcggacc gcctggcaca aggcgcagac
     4021 cgacgaaccc ctcctgatgc agggaaacgc cgcgcttcag tcgggggcct ttcgccgggc
     4081 gctcgacctc taccgcgccg cgctccgaga aaacccggtc tactgggtcg cctgggcgaa
     4141 cctcggcgac gcctacctcc gcatgggaag gcgcgacagc gctctggaga tgtaccgcat
     4201 tgccgacggc ctcaacccga gcagcccgat catcctgagc aacctcggcc aggcctggta
     4261 cttgaacgga caggccgaga agggcgtcga actgtggcaa cgcagcgcga gcctcgatcc
     4321 cgaattcctc cccccgctcc gtttcctggt caaccactac cgcacgagcc gcgaccggga
     4381 caactacctc gccgtactcg agcgcatcgc ccgcctcgac ggggcgccgg ccgacaacct
     4441 cgccgccctc gccgactggt acgtgcagag taatcgcttc gccgacgccg gccgactcta
     4501 tgaacgggcg ctggccgccg gcctcgacag cgcctacgtg tccgagatga tggccaaata
     4561 cccggttctg cgggaggagt ttccggcatg gcacacgacg agcaccgcat tctgacctgg
     4621 tccctgatca ttttcgtgct gactcttgcg gcgcaccttg tcgccgcgtc gcttcccgaa
     4681 atgcgcctct ggggggcggc ggcctgggcg cgcgtcgctc cggccctgtg ggccgcggcc
     4741 gttctgtccg ccctcgtgct cctcgccgcg tcgtttcgtc tgcccgccgc cgggcgcgcc
     4801 ccggcgggcc ggcgctacgt tctcgccgcc gccgccgcgg gactggcaac cgtcgccctg
     4861 ttctatctcc tgcgcaccaa ggcgcacttc ctcggcgacg gctacaccgc cctgagcctc
     4921 cttgccgacg acagcccgct gatcaagacg cgggaaatcg gcgaatcgct ggcgcacgcc
     4981 tgggtcaagc gcctgtccgg tgcggagggc gaggcggggg cgttgttcag tttccggttc
     5041 atctcgatcg ccgccggcgc gctcctcttc ggcgccgccg cctggagcgc cgcccgcctc
     5101 ttcgacagcg ccgcgcggcg ggcgctcttt gtggccggcg tcatgaccgg cggctacgcc
     5161 ctcctctttt tcggctacgt cgagaactac tccctctttg tcgcctcgac cggcatctat
     5221 gccctcgtcg gtctgctggt ggtgcgcggg caggttgccc gatggtggct gctgatcccg
     5281 ctcgccgcct cggcggcctg ccatgtgctc ggcgtcaccc tcctgcccag cgccgtctac
     5341 ctgctgctcg cccccacccg cgcgggcaag cgtctccgca cgtggcgggt atcgcaacgc
     5401 ctgacggtgg gcggtctgct tctcctggtc gctggctggg ctgtcgtcgg cgtgcggcag
     5461 tcgcacctgt ttttccgcta ctcgctcgtc ccgccgttcg aggatgcctt cacaatcggc
     5521 gggtatactc tcttttcacc cgcccacctg gccgacctgc tgaacctgct ggtgcttctg
     5581 ctgcctccgg tgggtctgct cgtcctcgtc atcgtgagcg ccgcccgctc ccgccgcctg
     5641 gccgcgcgcg acgccgtctt cctcatcgtg ctctccctct cggcgctcgg cgcagccttc
     5701 atttttgacc cgaaactcgg catgccccgt gactgggacc tcttcgcttt cgccggaatc
     5761 gcccccgccg tgctgctcta tgtcctgact ctggatcccc aatcccccct gccgggcggc
     5821 ctgcgtgtcg ccgcgatggc tgtcgtcctt tcgctctttg cgctgggcgg ccggctggtc
     5881 gccgccaacg atcctcccat ccagatcgcc cgtttcaacg actacctgac ggtcgacccg
     5941 gaacgcgatc gcaacgcccg ctatctcctg ttgacctact acaaagaccg cggcgacagc
     6001 gccgccgccg accgggtgcg cgccgagtgg cgcgccgctt tccccctccg cgacaaactg
     6061 gacgaggcgc agcgcctcca ccgtgaggag aagctgccgg aagcgaccgc cctgttcgag
     6121 cagattgtcc gcgaggatcc gacgtatgcc gatgcctggg ccgggctggg gtggatgatg
     6181 gtgcgctacc gcgattggga cagcgccatt gcctgtctcg acatgtcgct cggtctcaat
     6241 ccctaccacg ccgccgcctg tagtaaccgc ggcctggctc atctgcagct cggccatgcc
     6301 caacaggcgg cccgggattt cgagcggtcc ctgtcgatcg accccaacgt ccccgccgtc
     6361 atgtacaacc tcgccgtcgc ctacctgcgc tgccgccgct acgaggacgc cggcagagtg
     6421 atcgcccgca tggcggcgat gccggacatg ctggccggcg aacacctgaa actgttcgaa
     6481 tggtatgcca cctatggtcc tctcccggcg gcggtcgcgc agctccgctt cgcgatcgac
     6541 aagggagccg attccgcgta cgtccagtac ctgctgcagc gttttccgga cgccgcgaag
     6601 gccttccggc ggtagccgcg gcccccgaat ccggttgacc ggagccggtt gcggtgctac
     6661 ctttcgcccc tatgaccagt cctgccgacc atacccggac cgaaaaactc ctccttgagg
     6721 cggcccggat cttcaacagc acgctcgaat acgagagcct gattgccgag gtgctccgcc
     6781 tggtcaacac ggcgctcaat tcggaggggg cgctcctgtt ccgggtcgac cacgaccgga
     6841 ccgacatcaa aatccggctc gtgcgcgccc gcgacaacga gatgttcctg tttcaccgcg
     6901 acttcggcac cggcatcatc ggctgggtcg cccgccaccg cgaaccggaa ctcgtctacg
     6961 atgccaccgc cgacccgcgc gtcgatccgg aaatcgaaag ccgcttcggc tccccgatcc
     7021 gctccgtcgt ggtcgtcccc ctgatcggcc gcggccacat gatcggcgtc gtcgaggcgc
     7081 tcaacaaggt tgacggcgtc ttcacgcccg ccgacctcga tatcctcgtc ggcctggcca
     7141 accagatcgc cgtggccatc gacaacgccc acctctaccg cgtcgcccgc cgcgaagcgc
     7201 tcgagaagga actcctcttc gagatcggga aaaaactctc cagctcgatc agccagaaag
     7261 aggtcatgcg ggagattctc cactcggtca agcgggcgat cgatttcgac gccggcggcg
     7321 tgtttgtcat cgagccggcc aaagacatca tcggcgaggt gtacaccgag ggttacgatg
     7381 atcccggttc ggaggagcgc gtcctcgtca agaccggcga ggggctgatc ggccatgtga
     7441 ccgctaccgg cgagccggtg attgtccccg atgtgtcggt cgacccgcgc tacatgagcg
     7501 cccgcgagac cacccggagc gagattgtcg tccccatgct cctgaacaac cgcgtcgtcg
     7561 gcgtcctcaa cctcgagagc gacacccccg gcgcctaccg ggtcgacaac ctctcgcttc
     7621 tccaggcgtt tgccgcccag gccgccgtct ccctcgaccg cgcccgcctg cacgaagaaa
     7681 tcctcgccgc caagcggctc gaggagcagc tcaaaatcgc ccgcgaaatc cagcagacct
     7741 tcctgcccga ggacgacccc accatccccg gctacgacgt ttccggccgc aacgtccctt
     7801 ccggccaggt cggcggcgac tactacgact tcatccacat cgtcgactac cagaccggaa
     7861 tcgccatcgc cgacgtgtcc ggcaagggga tcccggccgc cctcatcatg gcctcgttcc
     7921 gcgcctcgct catcgccgag atccgcaaca actactctat ccgcacgatc gccgccaagg
     7981 tcaacaacct tgtctacgag tcggtgaaag ccggcagttt cgtaaccggc gtctacggcg
     8041 tgctcgactc ccgcaaccac attttcacgt tcaccaactg cggccacaac ctccccttcc
     8101 tggtgcgcgc cggaggagcc gtcgagtacc tccgcgaggg ggggcagatc ctcggggtgt
     8161 cgcctaacgc gcagtatgag gagcgcccga tctggatcgg acccggcgac atcatcctgc
     8221 tctataccga cggcgtgacc gaggttttta ccgagaccgg cgaggagtac gggctccagc
     8281 gcctggtcaa cctcgtccgc gaccaccgcg agaagacgtc gcaggaactt gtggacatca
     8341 tccatgaggc cgtctcccgg tgggcgggac cgaaaaagct gcccgatgat ttcac
//
LOCUS       DAOPOE010000116         8338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_9549_length_8338_cov_5.055898, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000116 DAOPOE010000000
VERSION     DAOPOE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_8338_cov_5.055898"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKM94_12145"
     CDS             complement(<1..307)
                     /locus_tag="PKM94_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD67425.1"
                     /translation="MLPSEILKKIRRIEIRTKRLVNDLFSGEYHSTFKGQGMEFEEVR
                     QYEPGDDIRLIDWNVTARTGYPHIKKFREERELSVVLLVDASSSGRFGTRERFKSETA
                     "
     gene            complement(317..1306)
                     /locus_tag="PKM94_12150"
     CDS             complement(317..1306)
                     /locus_tag="PKM94_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOD67426.1"
                     /translation="MHSEIQEIQARVEGESAFVRRLTDQMAAVIVGQKYLVERLLIGI
                     LSNGHILIEGVPGLAKTLSVHTLASAIQAKFQRLQFTPDLLPADLIGTMIYNPQTAAF
                     SVKKGPVFANIILADEINRAPAKVQSALLEAMQERQVTIGETTFPLEEPFLVLATQNP
                     IEQEGTYPLPEAQIDRFMLMLKIGYPTPAEEREIMERNTGMGTARVEKVVTPGDILQA
                     RQVVRSIYVDDKVKDYIVNIIFATREPQKYGLGELKDLIAFGASPRATIYLNLAAKAH
                     AFLRGRGYITPEDIKAIGHDVLRHRVLLTYEAEAEEVTSDDVVTKVFDAVEVP"
     gene            complement(1336..2199)
                     /locus_tag="PKM94_12155"
     CDS             complement(1336..2199)
                     /locus_tag="PKM94_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOD67427.1"
                     /translation="MKAIGPGLAATILLLFETVAAEGLSVSQALDKSHLAFEDSAVFT
                     ITLSWPGPQFAYRFPEPLDPTFEKLQVTRFETAVASQGTGAEETTHKTFRYTLAPLAP
                     GTGRIAPVTISYVTYPDSIPGEAVTEPMTVAVERPAAAPEDKPRGRWWLWLAGALAAA
                     GAGAAVVIRKARRPAAAPPVKTPADTALEQLDRLHVEAAGDLKKFQAGLYRVLAEFLR
                     ARFNIDADVLDDSQLAQAVRGADLRADVRDQLAAWIVTARQDKFRPVAGAPGETVRRE
                     AEVREALQKVT"
     gene            complement(2304..3149)
                     /locus_tag="PKM94_12160"
     CDS             complement(2304..3149)
                     /locus_tag="PKM94_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOD67428.1"
                     /translation="MRRFLDTMRLIRAGNCALAAAGVGVGAYMTWVEPVYLRPALAAA
                     ATFLMCAAGNAMNDLTDIVGDRINHPERVLVTGALAPEFARRLAIACAAAAAALGALA
                     GLEVAALVLLAAALLAAYNLHLKNLPVAGNLTVGVLGGMTFLAGGAAVDPTLAWILPG
                     PLIPAVFAVLFHFVREILKDARDIAGDRAVGSCSLPLALGIRASLTAALVLFAALVVL
                     TIVPVAAGWFGTLYKVITVYVIDFPVLALLILVWGNPTARMLSIGSTALKAGMALGVV
                     ALLVA"
     gene            complement(3188..4813)
                     /locus_tag="PKM94_12165"
     CDS             complement(3188..4813)
                     /locus_tag="PKM94_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD67429.1"
                     /translation="MKMARAWVWLWLAAGAAASPAREAGFEGPRPVNAAAVERAARQP
                     AVSLDSLERMLQTEGYMDAAVSQEEGRVRVRAGDRARLRVATVISGADTTALRLEAWF
                     REAEVTRLIDSLLDRRRECGYYYASITISGIERSGDDIMLYMSLVPGPVMRLGEVHYL
                     GLRRTKPATAVRYLTANAGDSLTDRYLVRLEREAAEIPWLRFLPPATVRPLPGFTSAD
                     VELQFVEERPLRLDVGGGYIPDTSEATVVWHVDARLNNLFGGGRRARVLSERRDKDRR
                     LLQVSYLQPLFFAGRAEGRIEAATRDYRTLFYEFALRAGYAVRLAQGVTAGVDLEYRG
                     VEPAEGGRSYDAYAAGFSARWDRVDEPLNPANGFEARTTISYGHRKYARGAAGEGERT
                     QYGETRTVFEARVYRRIAGPLAACAGLGYRGFETSEDFPPPAELNLIGGPGTLRGYRN
                     EQFAALRAVLATIEPRIRFGAGYAFGFYDGAFLSNRVSAAGGGRTEESYRSGFGGGVA
                     LTDGVRAVTVALGWNPEVPFREPRLSIEVRSGF"
     gene            complement(4813..8046)
                     /locus_tag="PKM94_12170"
     CDS             complement(4813..8046)
                     /locus_tag="PKM94_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67430.1"
                     /translation="MPRVTLAVFLWCTAALPLAAARVTLVGEAIPRWYPTGSRLVISG
                     SDSLFFNGRLLAAGREYRFDAGRRGFDLSDLHAQPGDTLVLTYAPAPSWLAARYGRPL
                     PEIVPPGGGTPALEPPRRTAPRKASGGLALSGAKSFRFAARSAGTADFSQSLDVTVAG
                     ELTPGLMIRGAVSDRGYDAAYGTVNSRLNELNKINLELESARFLARVGDIELRHRLAA
                     LQGRTARVSGASVTVREPRWYVETAAARPKGLHQTARFPGEDGVQGPYRIGPRAQPIV
                     PGSETVWLDGEKLARGSNNDYTMDYPAGSITFNVNHPIDRRRRIEIDYEVQATDYREE
                     VYSGGGGAALGDSTFVLETGWLREGDDRGSPLGGELSESDRAILEAAGDSAAPAYRSG
                     VRPDTAGAYVLVADSLPDSVYQYVGAGEGDYTVTFSYVGAGDGAYRNLGGNRFLYAGA
                     GNGDYLPVVALAAPERTDQYVARGRWRDRALGEVTAEYRRSEQDRNLFSPRGDGDNGG
                     NLWALGYARPWGEPGRGDGITLQSRFREGTFRTRERLYEPEFNRRNHLPDDYEPASDE
                     WRHEGTASFSPAEAVTLAPSGSLVRCDDGTEAESGGMRATVRPGRGVEVAGGVRSATT
                     TVVGAGRRSDGAARTIDGGVQYEAGKGWRIETGAERDERTNSYAGPRRGTRFVRYRLA
                     AGQRTEQVQAEHYVEDTLTGNWREALRRTRLELRSTRRIRTLAYQATAAYQWLDQPET
                     REESFLGRLNLQYADPGRRVMAAATYLLSDETRNVRGLSYLEVEPGRGDYVWEDGQYV
                     PDPNGNYVRVEEILSDRAAVSRGEKSFSFSKDWDGLLVRFTSRTEEELLAQGKRTALW
                     VLPFYSDPDEAYQFYSSRYAAEVRLIPIANAYAVNVEASENREVRTIAGSNRERRDRR
                     GTVTFRQAAGQRSFEEAVTLFDADRDAYYSGGGVIEGWQAEAAIRQLIGLHEVSGGSG
                     YRRASGAGGEESRQYLLRGGLRLAVVSRGEVRSTIELYRQELSGVAGLPSYTLTDSRY
                     GRRGAIWSLEFRYGVREDFRVSAAIDGRHSDDRTARVTARGEMVAGF"
     gene            complement(8068..>8338)
                     /locus_tag="PKM94_12175"
     CDS             complement(8068..>8338)
                     /locus_tag="PKM94_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67431.1"
                     /translation="VRTIAHDAWARPKAAAPAGGGLWVLDGAGRVYFCDARGGILFAA
                     GPMLPGSDSPLREPSDLAVLADGRLLIADTGNNRLLVCRVISDAG"
BASE COUNT         1269 a         3308 c         2478 g         1283 t
ORIGIN      
        1 ccgccgtctc gctcttgaac cgctcgcgcg tcccgaaccg gcccgaagac gacgcgtcga
       61 ccaacagcac caccgacagc tcgcgctcct cgcggaactt cttgatgtgc gggtaccccg
      121 tccgcgccgt cacgttccag tcgatcagcc ggatgtcgtc ccccggctcg tactgccgca
      181 cctcctcgaa ctccatcccc tgccccttga acgtcgagtg gtactccccg gagaacaggt
      241 cgttcacgag ccgcttggtg cggatttcga tccgccggat cttcttgagt atttcgctcg
      301 gcagcataaa cgaatcttac ggcacttcca cagcgtcgaa aaccttcgtc accacgtcgt
      361 ccgacgtgac ctcctcggcc tccgcctcgt aggtcaggag gacccggtgg cgcagcacgt
      421 cgtggccgat cgccttgatg tcctcggggg tgatgtaccc gcgcccgcgc agaaaggcgt
      481 gcgccttggc cgcgagattg aggtagatcg tcgcccgcgg cgaggcccca aacgcgatca
      541 gatccttcag ctcccccagc ccgtacttct gcggctcgcg cgtcgcgaag atgatgttga
      601 cgatgtagtc ctttaccttg tcgtcgacat agatcgagcg caccacctgg cgcgcctgga
      661 gaatgtcccc cggggtgacc accttctcca cccgcgccgt ccccatcccg gtgttccgct
      721 ccatgatctc ccgctcctcc gcgggcgtcg ggtacccgat cttcagcatc agcatgaacc
      781 ggtcgatctg cgcctccggc agggggtagg tgccctcctg ctcgatcggg ttctgggtcg
      841 ccagcacgag gaacggctcc tcgagcggga aagtcgtctc cccgatcgtc acctgccgct
      901 cctgcatcgc ctcgaggagg gccgactgca cttttgccgg cgcccggttg atttcgtcgg
      961 cgagaatgat gttcgcgaaa accggcccct tcttgaccga gaacgccgcc gtctgcggat
     1021 tatagatcat cgtgccgatc aggtcggccg gcagcaggtc gggggtgaac tgcagccgct
     1081 ggaacttggc ctggatcgcg ctcgccagcg tgtgcaccga cagcgtcttg gccagccccg
     1141 gcacaccctc gataagaatg tgcccgttcg acaggattcc gatcagcagc cgctccacca
     1201 gatacttctg cccgacaatc acggccgcca tctggtcggt cagccgccgg acgaacgccg
     1261 attcgccttc cacccgggcc tgaatttcct gtatctcgct gtgcatgcaa cctccatcta
     1321 tacggattca actcgtcagg tcaccttttg cagcgcctcg cgcacttcgg cctcgcggcg
     1381 cacggtctcc cccggcgccc ccgccaccgg tcggaatttg tcctgccgcg cggtcacgat
     1441 ccaggcggcg agctggtcgc gcacatcggc ccgaaggtcc gccccccgga ccgcctgcgc
     1501 caactggctg tcgtccagca cgtcggcgtc gatattgaac cgcgcccgca gaaactcggc
     1561 gagcacgcgg tacagacccg cctggaattt cttgaggtcg ccggccgcct cgacgtgcag
     1621 gcggtcgagc tgctccagcg ccgtgtccgc cggggtcttg accggcgggg ccgccgcggg
     1681 ccgccgcgcc ttgcggatca cgaccgccgc ccccgccccc gccgccgcca gcgcgccggc
     1741 cagccacagc caccaccgtc cgcgcggctt gtcctccggc gccgccgccg gccgctccac
     1801 cgccaccgtc atcggctccg tcaccgcttc ccccgggatg ctgtcggggt aggtcacgta
     1861 ggaaatcgtc accggcgcga tccgccccgt ccccggagcc agcggcgcca gggtgtaccg
     1921 gaaagtcttg tgggtggttt cctcggcccc cgttccttgc gatgcgacgg cggtctcaaa
     1981 ccgcgtcacc tgcagtttct cgaatgtcgg atcgagcggc tcggggaaac ggtaggcgaa
     2041 ctgcggcccc ggccaggaaa gggtgatcgt gaacaccgcg ctgtcctcaa acgccaggtg
     2101 cgacttatcc agcgcctgcg acaccgacaa cccttcggcc gcgacggttt caaaaagtaa
     2161 gagaatggta gctgccaggc cgggcccaat cgctttcatg ctgctgcaca ccgtggtctt
     2221 cgcatctgcg tataccccgc ctgcctgagg cgggccgaac atcgtgttat accggcgagg
     2281 aaccgtggcg gtttcacaga aagttaagca accaacagcg cgacaacgcc taacgccatt
     2341 cccgccttga gcgccgttga cccgatcgac aacattcgcg ccgtcggatt cccccacacc
     2401 aggatcagca gcgccagcac cgggaaatcg atcacgtaga ccgtgatcac cttgtacagt
     2461 gtcccgaacc agcccgccgc caccggcacg atcgtcagca ccaccagcgc tgcgaagagc
     2521 accagcgccg ccgtcagcga ggcccggatt ccgagcgcca gcgggagcga acacgatccc
     2581 acggcccggt cccccgcgat atcgcgcgcg tccttcagaa tctcccgcac gaagtggaac
     2641 aggaccgcga acaccgccgg gatcagcggc cccgggagga tccaggccag cgtcggatcc
     2701 accgccgctc ccccggcgag aaacgtcatc cccccgagga ctcccaccgt cagattcccc
     2761 gccaccggga gatttttcaa gtggaggttg taggcggcca gcagtgccgc cgccagcagc
     2821 acgagggcgg ccacttccag cccggccagc gctcccagtg cggcggccgc ggccgcgcac
     2881 gcaatcgcca gccgccgcgc aaactccggc gccaaggcgc cggtcaccag cacccgctcc
     2941 gggtgattga tgcggtcgcc cacgatatcg gtaaggtcgt tcatggcgtt tccggcggcg
     3001 cacatgagga acgtggccgc cgccgccagc gccggccgca ggtagaccgg ctccacccag
     3061 gtcatgtacg cccccacccc gacccccgcc gccgccagcg cacagttgcc cgcccggatc
     3121 aggcgcatgg tatccagaaa tcgccgcatg ccctgagtct aggcgcccgg ccggggagcg
     3181 tcaactttta aaaccccgag cggacttcga tcgacagccg gggctcgcgg aaagggactt
     3241 ccggattcca ccccagcgcc accgtcacgg cccgcactcc gtcggtgagc gccaccccgc
     3301 ccccgaatcc cgaccgatac gactcctccg ttcgcccccc cccggccgcg gacacacggt
     3361 tgctcagaaa ggccccgtcg tagaacccga atgcataccc cgccccgaac cgtatgcgcg
     3421 gttcgatcgt cgccagcacc gcccgcagcg ccgcgaactg ctcgttccga tacccgcgca
     3481 gcgtccccgg acccccgatc aggttgagct cggccggggg cggaaaatcc tcgctcgtct
     3541 caaacccccg gtatcccagc ccggcgcacg ccgccagcgg ccccgcgatc cgccggtaga
     3601 cccgggcctc gaacaccgtc cgcgtctcgc cgtactgcgt ccgctctccc tccccggccg
     3661 cgccgcgcgc atacttccgg tgtccatagc tgatcgtcgt ccgggcctcg aaaccgttgg
     3721 ccgggttcag cggctcatcg acgcggtccc agcgggcgct gaaccccgcc gcgtacgcat
     3781 cataggaccg ccctccctcg gccggctcca ctccgcggta ctccagatcg acccccgccg
     3841 ttaccccctg cgccagccgg actgcgtatc ccgcgcgtag cgcgaactcg taaaacaacg
     3901 tccggtaatc ccgcgtcgcc gcttcgatcc gcccctccgc ccggccggcg aagaacagcg
     3961 gctgcaggta gctcacctgc aggagccggc ggtccttgtc ccgccgctcc gacagaaccc
     4021 gcgcccggcg gcccccgccg aacaggttgt tcagccgcgc atcaacgtgc cacacgaccg
     4081 tcgcctcgga tgtatcgggg atatatcccc cacccacatc gagccggagc ggccgctctt
     4141 ccacgaactg cagctccaca tccgccgagg taaaccccgg aagcggccgc acggtggccg
     4201 ggggaaggaa gcgcagccac gggatctccg ctgcctcgcg ctccaaccgc acgaggtagc
     4261 gatcggtcag gctgtcgccg gcgttcgcgg tcagatagcg cactgcagtc gcgggcttcg
     4321 tgcgccgtaa tcccaggtaa tgcacttccc cgagccgcat cacgggtccg gggacgaggc
     4381 tcatgtataa catgatgtca tcgccggatc gctctatccc gcttattgtt atcgaagcat
     4441 agtagtagcc gcattcgcgc cgccggtcca gcagcgaatc gatcaggcgc gtcacctcgg
     4501 cttcccgaaa ccacgcctcc aatcgaagcg ccgtggtgtc cgctccgctg atcaccgtcg
     4561 ccacccgcaa ccgggctcgg tccccggccc gcacccgcac ccggccctcc tcctggctca
     4621 ccgccgcatc catgtaacct tccgtctgca gcatgcgctc cagcgagtcc agtgacactg
     4681 ccggctggcg cgccgcccgc tcgacggcgg cggcgttcac cggtcggggc ccttcgaacc
     4741 ccgcctcccg ggccggcgag gcggccgccc cggccgccag ccagagccac acccacgcgc
     4801 gcgccatctt catcagaacc ccgccaccat ttcaccgcgg gcggtcaccc gcgccgttcg
     4861 atcatccgag tgccgcccgt cgatcgccgc actcacccgg aaatcctccc gcaccccgta
     4921 gcggaactcg agcgaccaga tcgccccccg gcggccgtac cggctgtccg tgagcgtata
     4981 cgagggcagc cccgccactc cgctcagctc ctggcgatac agctcgatgg tcgaacgcac
     5041 ctccccccgg ctgaccaccg ccagccgcag gcctccgcgg agaaggtact gccgcgactc
     5101 ttcgccgccc gctcccgagg ccctccgata tccgctccct ccgctcacct cgtgaaggcc
     5161 gatcaactgc cggattgccg cctcggcctg ccagccctcg atcacccccc cgccggagta
     5221 gtaggcatcg cgatcggcgt cgaacagcgt caccgcctcc tcgaaactcc tctgccccgc
     5281 cgcctggcgg aaagtcaccg ttccccggcg gtcgcggcgc tcccggttgc tccccgcgat
     5341 cgtccgcacc tcccggttct cactcgcctc cacattgacc gcgtaggcgt tcgcgatcgg
     5401 gatcagccgc acctccgcgg cgtagcgcga gctgtagaac tggtacgcct cgtcgggatc
     5461 ggagtagaac ggcagcaccc agagcgccgt gcgttttccc tgcgcgagga gttcctcttc
     5521 cgtccgcgag gtgaaccgca cgagcaggcc atcccagtcc ttgctgaacg agaagctctt
     5581 ctccccccgc gacacggccg cccggtccga caggatttcc tccacccgga catagttccc
     5641 gttcgggtcg ggcacatact gcccgtcctc ccacacgtag tccccccggc ccggttccac
     5701 ctcgaggtaa gacagcccgc gcacattccg cgtctcgtcg gagaggaggt aggtcgcggc
     5761 cgccatcacg cgccggcccg ggtcggcgta ctggaggttc agccgcccga gaaacgactc
     5821 ctcccgagtt tccggctgat ccagccactg gtaggcggcg gtggcctggt aggcgagtgt
     5881 ccgaatgcgg cgggtcgagc gcagttccaa ccgcgtgcgc cggagcgcct cacgccagtt
     5941 cccggtcagc gtgtcctcga catagtgctc cgcctggacc tgctcggtcc gctggccggc
     6001 cgccagccgg tagcgcacaa accgcgtgcc gcggcgcggc ccggcgtacg aattcgtgcg
     6061 ctcgtcccgc tccgctccgg tctcgatccg ccatcccttg cccgcctcat actgcacccc
     6121 gccgtcgatc gtccgcgccg cgccgtccga gcgccggccg gcgcccacca ccgtcgtcgt
     6181 cgccgaccgg acgccccccg cgacctccac gccgcgcccc ggccgcaccg tcgcccgcat
     6241 ccctccgctc tccgcctcgg tcccgtcgtc gcagcggacc agcgacccgc tcggcgcgag
     6301 cgtcaccgcc tcggcgggac tgaaactcgc cgtgccctcg tgccgccact cgtccgatgc
     6361 cggctcgtag tcgtccggca ggtggtttcg gcgattgaac tccggttcgt agaggcgctc
     6421 gcgcgtacgg aaggtcccct cgcgaaagcg cgactgcagg gtgatcccgt ctccgcgccc
     6481 cggctctccc catggccgcg cgtaacccag cgcccagaga ttccccccgt tgtcgccgtc
     6541 cccccgcggg ctgaagagat tgcggtcctg ctcgctgcgg cggtactcgg cggtcacctc
     6601 gccgagagcg cggtcccgcc atcgcccccg cgcgacatac tggtcggtcc gctccggcgc
     6661 cgccagcgcc accaccggga gatagtcccc gttccccgcg cccgcataga gaaaccggtt
     6721 tccgcccagg ttccgatagg cgccgtcccc agccccgaca tagctgaacg tcaccgtgta
     6781 atccccctcc cccgccccga cgtactggta gacgctgtcg gggaggctgt cggccaccag
     6841 cacgtaggcc cccgccgtat ccggccgcac gcccgaccgg taggccggcg cggcgctgtc
     6901 gcccgccgcc tccagaatcg cccggtcgct ctcggagagt tctccgccga ggggcgaccc
     6961 gcggtcgtcg ccctcccgca gccagccggt ctccaggacg aaggtcgaat cgccgagcgc
     7021 cgccccgccc cccccgctgt acacctcctc ccggtaatcg gtcgcctgca cctcgtagtc
     7081 gatttcgatc cgtcggcggc ggtcgatcgg atggttcacg ttgaacgtga tcgaccccgc
     7141 gggataatcc atcgtgtagt cgttgttgct tccccgggcg agcttctcgc cgtcgagcca
     7201 gaccgtctcg gatcccggga cgatcggctg cgcccgcggg ccgatccggt aggggccctg
     7261 gaccccgtcc tcgcccggga accgcgccgt ttggtgcagc cccttgggcc gggccgccgc
     7321 cgtttcgaca taccaccggg gctcgcgcac cgtcaccgac gcgccgctca cccgcgccgt
     7381 ccgcccctgg agcgccgcca ggcggtgccg cagttcgatg tcgccgaccc gcgcgaggaa
     7441 gcgcgccgac tccagttcca ggttgatttt gttcagctcg ttcagacggc tgttcaccgt
     7501 cccgtacgcc gcgtcatagc cgcggtccga gaccgctccg cggatcatca gcccgggcgt
     7561 gagttccccc gccacggtca cgtcaagcga ctggctgaaa tccgccgtcc ccgccgaccg
     7621 cgccgcaaac cggaacgatt tcgctcccga gagcgccagc ccgccggagg ctttccgggg
     7681 agcggtccgc cgcggcggct cgagcgcggg tgttcccccg cccggcggca caatctccgg
     7741 caggggccgg ccgtagcggg cggccagcca tgacggcgcc ggcgcatagg tgagcacgag
     7801 cgtgtccccc ggctgggcgt gcaggtccga cagatcgaac ccccggcgcc ctgcatcgaa
     7861 ccggtactcc cgcccggccg ccagcagccg cccgttgaaa aacagtgaat cggatcctga
     7921 gatcacgaga cgcgatccgg tcgggtacca gcgcgggatc gcttctccca ccagcgtcac
     7981 ccgcgccgcc gccagcggca gcgccgccgt acaccagagg aaaaccgcca gggtgactcg
     8041 tggcatcggc gacccttcgc tccggcgcta cccggcgtcg ctgatcaccc ggcagaccag
     8101 caggcggttg ttccccgtgt cggcgatcag cagccgcccg tcggcgagca ccgccaggtc
     8161 cgagggctcg cgcagcggcg agtcgctgcc cggcagcatc ggcccggccg cgaacagaat
     8221 cccgccccgc gcgtcgcaga agtacacccg ccccgcgccg tcgagcaccc acaatcctcc
     8281 cccggccggt gccgccgcct tcggccgcgc ccacgcgtcg tgcgcgatcg tccgcacc
//
LOCUS       DAOPOE010000117         8307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_9598_length_8307_cov_4.552351, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000117 DAOPOE010000000
VERSION     DAOPOE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9598_length_8307_cov_4.552351"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKM94_12180"
     CDS             complement(<1..377)
                     /locus_tag="PKM94_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67432.1"
                     /translation="METASPVRQPWCDLVWGPSAAAQAAAARDIIAIVDTLSFSTATA
                     AAAAAGGLIYPCRSYDDAVAMAARVKASPAVRRSDVPARGRFSLSPLTYRGLQPGERV
                     VLPSVNGGVCTRAAVSGGHIVLGA"
     gene            complement(449..2602)
                     /locus_tag="PKM94_12185"
     CDS             complement(449..2602)
                     /locus_tag="PKM94_12185"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOD67433.1"
                     /translation="MPDHSRTDHHQIILGARRPPPSRLCWAAALGAFAAAMAVYGSTA
                     YPTIAWWSSAELALAAHELGVMAPPGGVIPTLAGWVVLHVVPGEMGVFALNLFAGLLA
                     AGVVAGAILLGCRLEWISHATASARGGAFAALAVMTAAGATGLALAFGETLWTYALHY
                     SEYVVTPLFTVLIVAALVRWWEAAPKTGGYGWLAVATLLLGLDLSVHRTNALLLPALP
                     FWVLLRHPRGLLSAGTWLAGIGGLAAGLAFHLVLIPMARAHPFLNLGDPQSLDRWWSY
                     VSLEMRGGGFLVNLYPRNADFWSVQVSDWVTAFTRTFASADGPLGVVGFVPLGLGVYG
                     LAVILRRSFRLGLGLTLLFAAASLGAIVYFNIPAGYFRSLHRHYLPSLVIFGGLAVAG
                     AAALVRAALRLEGLPGRLVAALTAACILLAAGNGLWRNYGQLDASADQFAWEYASNLL
                     APLPPDAILLTQGDNDTFPLWYLQRVEGVRPDVTVLNVPLLNTPWYVEQVRRWDPRLP
                     LEFSAAELAAYAPVVWRDSTVAAPAGGTAADYELPESASVPDTVRLLVRPTAGPHLLL
                     AQDQAVLRIIQGNTGRRPLYAVSGLGQQALPWLGPYLRLEGLVQRFVPVEHPALDPTL
                     LARNLFEAYSYEGFAHVPPTVEPAAGDYLQQLGSAYVILFGAYASGGDAAGMERVCAQ
                     VFTRLNLDRLLPGAPFLQALEEPCRGLRPPAADGQ"
     gene            complement(3011..3280)
                     /locus_tag="PKM94_12190"
     CDS             complement(3011..3280)
                     /locus_tag="PKM94_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67434.1"
                     /translation="MNDQFDEIWKRIVEHAGEQFQTRAGRTFRYVIEEGDRVRPTLTD
                     FSVSRAEIRQYFLDIAEASTGKIAAVSGGPSFVWAILTDVRIMRH"
     gene            3857..4849
                     /locus_tag="PKM94_12195"
     CDS             3857..4849
                     /locus_tag="PKM94_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67435.1"
                     /translation="MPRYLPVIASACCFLLMLPAQSSGQEDCSADFDVDGDGQPLSIN
                     DFVTAVRALAGDTTVAVAWSHLDLNGDCRIDGADLELAACYFESGLACFPDGFPVPTC
                     CQPDTVRGACCLPPDSCSVRHPANCAQLGGIYMGDWSACAAYLCDCCEGVRDDINGDG
                     AFKVADATFFIAFLFRGGLEPPCFEEADLNADGKLDINDLVGSLIPALFHPGTWMWLP
                     CPGADLPADITFEVRWYDTPTGGLSRMDTLYLTLLGPGALHGTFEPGVPEDSVAGTWY
                     ADGSLYLANAIGLADAGFVTRQYRSGDTWAGIWEFTTIAGPRRLPGTMRPLPSI"
     gene            complement(4907..6166)
                     /locus_tag="PKM94_12200"
     CDS             complement(4907..6166)
                     /locus_tag="PKM94_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD67436.1"
                     /translation="MPLTKFRTALEAELAALEGQGLSKAHEHVIVRVHRPGGRVGTRV
                     FLEGHGDRPFLRMNANNYLGLSTHPRPIQAEEETVHSMGVGPGAVRFIAGTFQPHVEL
                     EEALARFHGREACMITSAAYTAVLGVLAALTTPETVIISDELNHNCIINAMKLARPKE
                     RKIYRHLDPADLERHLAASVGSADCALVVTDGVFSMRGDIAPLDVLQRIVERYDEKFP
                     RGAVLLVDDSHGVGTLGAAGRGAEELTRGRGDILIATLGKAFGVNGGYIASEASVVAY
                     LREKNPLYIFTNPITPPEAAAALQALAVVDSAEGRRLIAHLHFLTQRFEQGLVDLGFE
                     TISSPHPVVPLIVRDTERTRELVRYLYEHNILATGLNYPVVPRGEELIRFQVAADLTV
                     EDIDLVLDVLRAYRGWRSGTADTRRAG"
     gene            complement(6374..7621)
                     /locus_tag="PKM94_12205"
     CDS             complement(6374..7621)
                     /locus_tag="PKM94_12205"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOD67437.1"
                     /translation="MGFSDRVRATYQQQIRELQEAGLFKQERFIHSMQSPDIEVEFPS
                     GAPLKKVINMCANNYLGLSSHPSVVQAAHEGLDTRGYGMSSVRFICGTQDIHRELEDR
                     LSAFLGTEATILFPSCMDANAGVFEAILGKDDVMISDRLVHASIIDGIRLCSAVQDTF
                     KHSNMEHLEEKLQLHADKRCKVIITDGVFSMDGDTAKLDEMVALAEKYDAMVFVDDSH
                     STGFIGKTGRGTHEKCGVFGRIDIITTTFGKALGGASGGCVSGRRELVEMCRQRARPY
                     LFSNTIAPVVVAGVLKVLEILSASTERRDKLEANTAYWRKGLSEAGFVLKEGDTPIVP
                     VMLFNAKLSQDFSRDLFQEGIYAIGFFFPVVPQGQARIRTQISAAHERHHLDKALDAF
                     TKVGKKYNILGKTKQEIIEMFGQ"
     gene            complement(7694..7939)
                     /locus_tag="PKM94_12210"
     CDS             complement(7694..7939)
                     /locus_tag="PKM94_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD67438.1"
                     /translation="MDEMTKVVLDYVRREYLEDEDDELDVHTPLISGGIVDSFSMVSL
                     KRFLENKYKISIPDEKATPEAFDSVTKIVSLVREYVK"
     gene            complement(7975..>8307)
                     /locus_tag="PKM94_12215"
     CDS             complement(7975..>8307)
                     /locus_tag="PKM94_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOD67439.1"
                     /translation="GHLVSPFEVESALLEHPAVAESAVVSKPDPINMEVVKAFVTLKP
                     GHQPGADLELEIMNFIRKKLSPLAMPQEIEYVPGLPKTRSGKIMRRLLHAREWGEEVG
                     DVSTLEND"
BASE COUNT         1458 a         2823 c         2636 g         1390 t
ORIGIN      
        1 gcgccgagga caatgtgccc ccccgaaaca gcggcccggg tgcacacgcc gccgttgacc
       61 gagggcagca ccacccgctc gcccggctgc aggccgcggt aggtcagcgg cgagagcgaa
      121 aaccgcccgc gcgcggggac gtcgctgcgc cgcacggccg ggctggcctt cacccgcgcc
      181 gccatcgcaa ccgcatcatc gtaggatcgg caggggtaga tcagtcctcc ggctgcggcc
      241 gccgccgctg tcgccgtgga aaaactcagg gtatcgacga ttgcgatgat gtctctcgcc
      301 gcggccgcct gggcggcggc cgacggtccc cagacaagat cgcaccaggg ctgcctgacc
      361 ggcgacgctg tctccaacga aagtctcctt ggattcggac gggctctgta cccgccgcca
      421 tcctaaaacc tgttgggcgg aaaaatgttc actgcccgtc cgcggccggc ggtcgaaggc
      481 ctcggcaggg ttcctcgagc gcctgcagga aaggcgcacc cggcaggagc cggtcgaggt
      541 tcaggcgcgt gaacacctgt gcgcacacgc gctccatgcc tgccgcatcg ccccccgacg
      601 catacgcccc gaacaaaatc acatacgcgc tgcccaactg ctgcagataa tcgcccgctg
      661 ccggctccac ggtcggcggc acgtgcgcga aaccttcgta ggaataggcc tcaaagaggt
      721 tgcgggcaag gagcgtcgga tcgagcgcgg gatgctcgac cgggacaaac cgctggacca
      781 gtccctccag acgcaggtac ggccccagcc acgggagcgc ctgctgcccc agaccggata
      841 ccgcatagag cggccgccgc ccggtattgc cctggataat ccgcagcacc gcctggtcct
      901 gagccagcag caggtgcggc ccggccgtgg gacggacgag cagacgcacg gtatccggca
      961 cggacgctga ctcgggaagc tcgtagtcgg ccgctgtacc gccggccggc gccgccactg
     1021 tcgagtcgcg ccacaccacc ggggcgtaag cggccaactc cgcggcggag aactccagcg
     1081 ggagccgggg gtcccacctc ctcacctgct cgacatacca gggggtgttg agaagcggga
     1141 cgttcagcac cgtgacatcc ggccgcaccc cctctacccg ctgcaggtac cagaggggaa
     1201 aggtatcgtt gtccccctgg gtgagcagga tagcatcggg tggcagcggc gccagcaggt
     1261 tggaggcgta ctcccaggcg aactggtcgg cggacgcatc gagctgcccg tagttgcgcc
     1321 agaggccgtt accggccgcc agaaggatac aggctgcggt gagtgccgcg accagtcggc
     1381 cgggcagccc ttccaggcga agcgccgcgc gcaccagcgc cgccgctccc gccaccgcca
     1441 gcccgccgaa aatcaccagc gagggcagat agtggcgatg gagcgaccgg aaataaccgg
     1501 cgggaatgtt gaaatagacg atcgccccca gcgacgccgc ggcaaacaac agcgtcaggc
     1561 cgagacccag acggaacgac cgccgcagaa tcaccgccag accgtacacg ccgagaccca
     1621 gcgggacaaa accgacgacg cccagcggac cgtcggcaga ggcgaatgtc cgcgtgaacg
     1681 cggtcaccca gtcgctcacc tggacactcc agaaatccgc gttgcgcggg tacagattca
     1741 ccaggaaccc gccgccgcgc atctccagcg agacgtagct ccaccaccgg tcaagcgact
     1801 gcggatcgcc gagattcagg aacggatggg cccgggccat cgggatcagc accagatgaa
     1861 atgccagccc ggctgccaga ccgccgatcc cggcgagcca ggtcccagcc gagagcagtc
     1921 cgcgcgggtg tcggaggagc acccagaagg gcagcgcggg gagcagcagg gcgttggtcc
     1981 ggtgcaccga cagatccagc cccaaaagca gggtggcgac cgccagccag ccgtacccgc
     2041 ccgttttcgg cgccgcctcc caccagcgca ccagggcggc gactatcaac acggtaaaga
     2101 gcggcgtaac aacgtactcg gagtagtgga gggcgtaggt ccagagcgtt tcgccgaatg
     2161 cgagcgccag ccccgtcgcc ccggccgccg tcatcaccgc caacgccgcg aaagctcccc
     2221 cgcgcgccga cgcggtggcg tgcgagatcc actccaagcg gcagcccagc agaatcgcgc
     2281 cggcgaccac gccggcggcc agcaaacccg cgaacaggtt gagggcgaac acgcccatct
     2341 cccccggcac aacgtgcagc actacccagc cggcgagggt cggaataacc ccccccggcg
     2401 gcgccatgac gcccagctcg tgggcggcga gggcaagctc tgcgctgctc caccaggcga
     2461 ttgtcggata ggcggtcgac ccgtacaccg ccatcgccgc ggcgaacgct cccaacgcgg
     2521 ccgcccagca taaccgggac ggcggcggtc ggcgagcccc caggatgatc tgatggtgat
     2581 cggttcggct gtggtcgggc atacgaaaat ctcctcccaa cagttcgtgg gtcgggcaca
     2641 acatcgactc tgggcagaat catgttcggg tgggtcgcca accgaacaca agctttaacc
     2701 ggaggaaaac aaacaccccc cggcgcttgc cggggggttg atgcgaaatt taacatgtgt
     2761 ggaagtcacc cgctatcggt gccgcaacag gcggcggggt cgggatcctt tcccgttttc
     2821 gcccctatag ccggtcttcc atatacatat acccatcaaa acccggtttt gtcaaggaaa
     2881 aatggacctt ccagggaaat ttctgactgt tgcggccggt cgccgacggc gccaccggcg
     2941 cttttctcgc acttccccgc cggatcttgt gggtcgcccc acccggaccg cccgttgcgc
     3001 ggcaatcggt tcagtgacgc atgatgcgga cgtcggtgag aatcgcccag acaaacgacg
     3061 gtccgccgct cacggccgcg attttccccg ttgaggcctc tgcgatgtcg aggaaatact
     3121 ggcggatttc cgcccggctc acggaaaaat cggtcagcgt aggccgaacc cggtccccct
     3181 cctcgatgac gtatcgaaat gtccgtcccg cgcgcgtttg aaactgctca ccggcgtgtt
     3241 caacgatccg cttccagatc tcgtcaaact ggtcgttcac aaccgctccc cgaattgatt
     3301 atgtcattgt ttcaatttgc gttggccccg ccgtcgcccg acttcgccga ctgagacaac
     3361 tccgaccatg cgcaagacgc tctgtctgag ccgtatctag ggagttcgta tcccacatgc
     3421 aatctttttt tcatacgagt caccacgtga cgcacgcccg ccaattttct tcagcagagc
     3481 ttgacaaccg agaatccgga gttggcattg aacccgagaa gaacaagaga acccgcaggg
     3541 cggacctccg tggaaaagtg cgatttgttg acctacctgc agacgcatgt gctcaagttc
     3601 ggctctcctc cgactgaccg cgtgccggcg cccccgcccg gtacccgctc ctcgagcgac
     3661 acgcagccgg agccgccgac acccgcctga ttttcgatct tggccgcacc agatgagcgc
     3721 cgcgcgtcga gcttggccgc gcctggccgg cggttgcgcg gcgcctcgcg cgcgcgaaag
     3781 ggcttgacaa ctccgccccg catggtagct taaaggtaga caactttctg ccacatgcta
     3841 tcagatcgga ggtgtgatgc ctcgatatct cccggtgatc gcgtcggcgt gctgcttcct
     3901 gttgatgtta ccggctcagt ccagcgggca ggaggactgc tctgcggatt ttgacgtcga
     3961 cggcgacggt cagccgctct cgatcaacga tttcgtgacc gccgtgcgcg ctttggccgg
     4021 cgataccacg gtggcggttg cgtggtctca tttggatctc aacggcgact gccggatcga
     4081 tggggccgat ctcgagttgg cggcgtgcta tttcgaatcc ggtctcgcct gcttcccgga
     4141 cggtttcccc gtgccgacat gttgccagcc cgacacggtg cgcggcgcct gctgcctgcc
     4201 gcccgattcg tgttcggtcc gccacccggc taactgcgcc cagctcggag ggatctacat
     4261 gggtgactgg tccgcctgcg ccgcctacct gtgcgactgc tgcgagggag tgcgcgatga
     4321 tatcaacggc gacggagcgt tcaaggtcgc ggacgccacc ttcttcatcg ccttcctctt
     4381 ccgcggcggg ctcgaaccgc cctgtttcga ggaggcagat ctcaacgccg acgggaagct
     4441 cgacatcaac gatctggtcg gctccctcat cccggccctc ttccaccccg gaacctggat
     4501 gtggctgcct tgtcccggcg cggacctccc ggcggatatc actttcgagg tccgttggta
     4561 cgacacgccg acgggcggcc tgtcgaggat ggacacgctc taccttacgc tgctcgggcc
     4621 cggggcgctg cacggaacat tcgaaccggg tgtccccgag gattcggtcg ccggaacctg
     4681 gtacgcggac ggctcgctgt acctggccaa tgcgattgga ttggccgatg ccggtttcgt
     4741 gacaaggcag tatcggagcg gggacacctg ggcgggaatc tgggagttca cgacgattgc
     4801 cgggccgcgc cggctgccgg gcaccatgcg tcccctgccg tcgatctaac gcatcgcagg
     4861 ggcggtcgcc gagtcgtccg cgcctttcgt ccgcccggcg ccgacgtcat cccgcccgac
     4921 gggtgtcggc ggtaccgctg cgccatcccc gatacgcccg caggacgtcg agcaccaggt
     4981 cgatgtcctc gaccgtaagg tcggccgcca cttggaagcg aatcagctcc tcccctcgcg
     5041 gcaccaccgg gtagttgagc ccggtcgcga ggatgttgtg ctcgtacagg tagcggacca
     5101 gctcccgcgt ccgctcggtg tcccgcacga tcagcggcac gaccgggtgg gggctcgaaa
     5161 tcgtctcaaa ccccaggtcg accagcccct gctcgaaccg ctgcgtgagg aaatgcaggt
     5221 gtgcgatgag ccgccgccct tcggcgctgt cgaccaccgc cagcgcctgc agtgccgccg
     5281 ccgcctcggg cggggtgatc ggattggtga atatgtagag gggatttttc tcgcgcaggt
     5341 aggcgaccac gctcgcctcg gaggcgatgt acccgccatt gacgccgaaa gccttgccca
     5401 gcgtggcgat cagaatgtcg ccgcgcccgc gggtcagctc ctccgcgccg cgcccggcgg
     5461 ccccgagcgt cccgacgcca tgggaatcgt cgaccagcag cacggccccg cgcgggaact
     5521 tctcatcgta gcgctcgaca atccgctgga ggacgtcgag cggtgcgatg tcgccgcgca
     5581 tcgagaatac gccgtcggtc accaccagcg cgcagtccgc gctcccgacc gaggcggcga
     5641 ggtggcgctc caggtccgcc ggatcgaggt gccggtaaat cttccgctct ttcgggcggg
     5701 ccagcttcat ggcgttgatg atgcagttgt ggttgagctc atcggagatg atgaccgtct
     5761 ccggggtcgt cagcgccgcg agcacgccca ggaccgccgt gtaggcggcc gaggtgatca
     5821 tgcaggcctc gcgcccgtgg aaccgcgcga gcgcctcttc gagctccacg tggggctgga
     5881 aggtgccggc gatgaagcgc accgcacccg gaccgacccc catcgagtgc accgtctcct
     5941 cctccgcctg gatggggcgg ggatgggttg acagcccgag ataattgttg gcgttcatgc
     6001 gcaggaaggg gcggtcgccg tggccctcca gaaacacgcg cgtgcccacc ctcccgccgg
     6061 gccgatgcac ccgcacaatc acgtgttcat gggctttgga gagcccctgc ccctcaagcg
     6121 cggccagttc ggcttccagc gcggtacgaa acttcgtcag cggcatcttt cgttcccggt
     6181 tatcccttct aaatcgcaga cggccggcgg ttccggtcgt cggcgcggca atgtatggtg
     6241 taacggggac ggcggcaagc ggttcccgcg ccgccgcccg acaaaaaaac tcaaggcagg
     6301 aaccccttac gggttcctgc cttggacact cagacggcgg ccggcagagc ggcggccgcg
     6361 ggacgcccgg acctcactgg ccgaacatct cgatgatctc ctgcttggtc ttgcccagga
     6421 tgttgtactt cttccccacc ttggtgaacg cgtcgagcgc cttgtcgagg tggtgccgtt
     6481 cgtgggcggc cgaaatctgg gtgcggatgc gggcctgccc ctgcggcacg accgggaaga
     6541 agaatccgat cgcgtagatg ccttcctgga agaggtcgcg ggagaaatcc tgcgacagct
     6601 tggcgttgaa cagcatcacc ggcacgatcg gcgtatctcc ctctttgagc acgaacccgg
     6661 cttcgctcag ccccttgcgc cagtaagcgg tgttggcctc gagcttgtcg cgccgctcgg
     6721 tcgacgccga caggatttcg agaaccttga gcacaccggc caccacgacc ggggcgatcg
     6781 tattggaaaa caggtagggc cgggcgcgct gccggcacat ctcgaccagc tcccgccggc
     6841 cggagacgca gccgccggag gcgccgccga gcgccttgcc gaaggtcgtg gtgatgatgt
     6901 cgatccggcc gaagacgccg cacttctcgt gcgtgccccg cccggtcttg ccgataaaac
     6961 cggtcgagtg cgaatcatcc acaaacacca tcgcgtcgta cttctccgcc agcgccacca
     7021 tctcgtccag cttggccgtg tcgccgtcca tggaaaacac gccgtcggtg atgatcacct
     7081 tgcaccgctt gtcggcgtgc agctgcagct tctcctcgag gtgctccatg ttggagtgct
     7141 tgaaggtatc ctgcacggcg ctgcagaggc ggatgccgtc gatgatcgag gcgtgcacga
     7201 ggcggtcgga aatcatcaca tcgtccttgc cgagaatcgc ctcgaacacc ccggcgttgg
     7261 cgtccatgca ggagggaaac aggatggtcg cctcggtgcc gaggaaggcg gagaggcggt
     7321 cttcgagttc gcggtgaatg tcctgggtgc cgcaaatgaa acggaccgag gacatgccgt
     7381 agccgcgcgt gtcgagccct tcgtgcgcgg cctggaccac cgaggggtgg ctcgagaggc
     7441 cgaggtagtt gttggcgcac atgttgatga cttttttcaa cggcgccccg ctggggaatt
     7501 ccacctcgat atcgggcgac tgcatcgaat ggatgaaccg ttcctgtttg aacaggccgg
     7561 cctcctgcag ttcccggatc tgctgctggt aggtcgcgcg cacgcgatcg ctgaaaccca
     7621 tgagtcttct ccttgccggc cggtcgcccc cgtcccgccc cggtggcggg tccggcgggc
     7681 ggccaccggg cgcttacttc acatactcgc gcacgagcga gacgatcttg gtcaccgagt
     7741 cgaacgcttc cggagtcgcc ttctcgtcgg gaatcgaaat cttgtacttg ttctccagga
     7801 accgcttgag cgagaccatc gagaaggagt cgacgatgcc gccggagatc agcggcgtgt
     7861 ggacatcgag ctcgtcgtcc tcgtcctcga ggtactcgcg cctgacgtaa tccagaacaa
     7921 ctttggtcat ctcatccatg gtagtctcct gctccacacg agcgtgccgc tcggttaatc
     7981 gttttccagc gtcgacacat cgccgacctc ctcgccccac tccctggcgt ggaggaggcg
     8041 gcgcatgatc ttgccgctcc gcgtcttcgg cagccccggc acgtactcga tctcctgagg
     8101 catggccagc ggcgacagtt tcttgcggat gaagttcatg atctccagtt cgaggtcggc
     8161 gcccggctga tgccccggtt tcagcgtcac gaacgccttc accacttcca tgttgatcgg
     8221 gtcgggcttg gacaccaccg ccgactcggc caccgcgggg tgttccagaa gggccgattc
     8281 cacctcgaac gggctgacca ggtggcc
//
LOCUS       DAOPOE010000118         8100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_9912_length_8100_cov_4.261280, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000118 DAOPOE010000000
VERSION     DAOPOE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_8100_cov_4.261280"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKM94_12220"
     CDS             <1..320
                     /locus_tag="PKM94_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67440.1"
                     /translation="ALPVYAAHPESKSAGLLVAELQMRARQGDAAYDVLMNLSRRFPT
                     DFSIQLQVGRFLLLAAIQKKDLNLQRTAERYFAESVRINPYRGKYAMNIWSQMLSGAQ
                     GQP"
     gene            583..873
                     /gene="groES"
                     /locus_tag="PKM94_12225"
     CDS             583..873
                     /gene="groES"
                     /locus_tag="PKM94_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOD67441.1"
                     /translation="MQIKPLADRVVVKPFEEAETVKGGIIIPDTAKEKPMQGEVVEVG
                     PGRVNEEGKKTPMEVKKGDRVLYGKYSGTEVTIGGTEYLIMRESDIFAIVQG"
     gene            911..2539
                     /gene="groL"
                     /locus_tag="PKM94_12230"
     CDS             911..2539
                     /gene="groL"
                     /locus_tag="PKM94_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521902.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOD67442.1"
                     /translation="MAKQIDYNTEARAKLKRGVDKLANAVKVTLGPKGRNVVIDKKFG
                     SPTVTKDGVTVAKEIELEDHFENIGAQMVKEVASKTSDDAGDGTTTATLIAQAIYREG
                     LKNVTAGHNPMAIKRGIDRAVEVVGGEIKKMSKPVSGKQEIAQVGTISANSDKQIGDL
                     IAEAMEKVGKDGVITVEESKSADTTLEVVEGMQFDRGYVSPYFVTDPDNMEAVLDEPL
                     ILIHDKKVSSMKDLLPILEKVAQAGRPLMIIAEDIEGEALATLVVNKLRGTIKVGAAK
                     APGFGDRRKAMLEDIAVLTGGKVISEEAGFKLENAVMSDLGHAKKVTLDKDNTTIVEG
                     GGQKEAIKARINQIRKQIEDTTSDYDREKLQERLAKLAGGVAVINVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRCMKALDSLKVTEEEQVGVMIVRKALEEPIR
                     QIASNAGAEASVVVDKVLAQTGAFGYNAQTDTYEDLLKAGVIDPTKVTRTALENAASI
                     AGLLLTTEAVVVEQPEEKKPAPAAPDMGGMGGMY"
     gene            2780..5467
                     /locus_tag="PKM94_12235"
     CDS             2780..5467
                     /locus_tag="PKM94_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOD67443.1"
                     /translation="MTLAKGHRLGPYEIEGKTGSGGMGEVYRAKDTRLDRTVAIKVLP
                     EHSALNPEARARFEREARTVSSLNHPHICVLHDLGRQEGVDFLVMEYLEGETLEDRLR
                     RGRLDLSEVLTIASQVAAALEAAHRKGLVHRDLKPGNIFLTEDGAKLLDFGLAKPQAE
                     FVQGLDGETRTTPVTGAGAIVGTLLYMSPEQLEGREADARSDIFALGATLYEMIAGER
                     AFTGDSKAGLIGAIMSEQPRAIVERLPSAPPVLDRLVRKCLQKSPDRRWQTAGDLKDE
                     LDWIASAGSQAGLAAPVALQRRFRLRLAWILAAVFALTAALLGAQWLTQKTPVARTMR
                     FRMPKPEGLARTDWPQLSPDGRYLSLLATDTSGETQIWIRPLSSPQPYPLPGTANARR
                     PFWSPDGEFLAFFDRSSDRLMKVPVAGGQPQIVCKTAGSDGTWGRNNIILFDDYRDYR
                     SIGQVSASGGEPRLAVAPDTAAGELTATWPCFLPDGQHFIFTCGSDSLRGTDQVFLKL
                     GDIDGGESRTLGTVESRAVFCEPEYVLYMKNGFLVAHRFNLKRLELEGEPVPLTDRVG
                     ISAGSNLGVNVAASATGILAWQTKGQAKSRMVWTDRAGVAQDTVGPPANYREIRLSPD
                     NRRIACTIAETGVEFTRLWVADVARGSVSILTSGAGNWFDATPLWFSDSRSLVYLSVS
                     ATDLTASLCRQLISRSEASVLRQSTGDGLLPLGWTASGNLIVGAIPLSAVSTDTAWRL
                     VAIDPEDTGRLDTLLTGGDAGVLYDVSPDGRYLLVGMTSGVESGLHVRDLLQPDNAWL
                     LPGSGTAARWSPAGDEIFYFEGDNLVALDIDLRGGFAFGAPDTLFRRHLVAGRTTLRG
                     YDVSYDGRRFLFVAPVGPEEQEPNEIEIVLNWNAELKRK"
     gene            5696..6655
                     /locus_tag="PKM94_12240"
     CDS             5696..6655
                     /locus_tag="PKM94_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD67444.1"
                     /translation="MNRREFLTEAGRLATLAIVAGQPGLISLGCQSAGYRRASGPKPD
                     FLVPPDPRLPGVVLARDEDHAAALGAALEGIGGIRRFIQPGERVLLKPNAAWDRVPAQ
                     AVNTNPLVVGEMVRQCRLAGAAEVLVTDHGGPRPEEAFTRSGIAAAVAQNGGRVIYLE
                     EDDFLEDDLGGRFIGAWPVLRHVFEVDRFINMPIAKHHGLVAGTASMKNFFGAIGGVR
                     DVLHDQLDQAIVDLAAYFRPTLTVIDATRVLMRHGPGGGAFEDVAVRNAVICATDQVA
                     GDSCAAGFLGAAGRDIRHIILAAQQGLGEIDYRRAGFLELGGI"
     gene            complement(6963..>8100)
                     /gene="eno"
                     /locus_tag="PKM94_12245"
     CDS             complement(6963..>8100)
                     /gene="eno"
                     /locus_tag="PKM94_12245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD67445.1"
                     /translation="DKSKYLGKGVQKAVDNVNEKIAPALLKDGIDPYDQVAVDNYLIG
                     LDGTENKGKLGANALLGVSLATAKAAAESRGRFLYEYIGGCNCKLLPVPMMNIINGGK
                     HADNNVDLQEFMIMPVGAGSLSEALRMGSEVFHHLKKVLAAKKYNTAVGDEGGFAPDL
                     KSNEEALQVIMEAIAKAGYQPGEQIRIALDPASTEFYDAKSGKYNLKAEGKKLSSSEM
                     IDFYERLCDKYPIISIEDGLAEDDWKGWKEMTERLGSRIQLVGDDLFVTNIKRLGRGI
                     AEGIANSILIKLNQIGTLTETLDAIRMAQHAGYTAVVSHRSGETEDATIADVVVATSA
                     GQIKTGSMSRTDRIAKYNQLLRIEEQIGKYAEYPGMNAFSSVRR"
BASE COUNT         1488 a         2784 c         2440 g         1388 t
ORIGIN      
        1 tggccctccc cgtctacgcc gcccacccgg aatccaagtc ggccggcctg ctggtcgccg
       61 aacttcagat gcgtgcccgg cagggggatg ccgcgtatga cgtcttgatg aacctctccc
      121 gccgcttccc gaccgatttc tccatccagc tccaggtcgg ccggttcctc ctcctggccg
      181 ccattcagaa gaaagatctt aacctccagc gcaccgccga gcgctatttc gccgaatcgg
      241 tgcgcatcaa tccctaccgg ggaaagtacg ccatgaacat ctggtcgcag atgctctccg
      301 gggcgcaggg tcagccctaa gcataatgag cgccgctatc gagccggtcc ggtgaaaaaa
      361 accggatttc cgcctgaaac ctcttgacaa gttccgcgtt acaattatat tcaagaccgc
      421 tgtcagcact cattgatagt gactgctaat agctgctaag aacatgagat tgaatagctt
      481 aagagcctgc agcaacgaag tgcggcccag ctaacatagc caaaactaat cgacataacg
      541 ttccggtaac cattggctga attcacagga ggtaaaaaag ccatgcagat taaaccgctg
      601 gccgaccgcg tggtcgtgaa gcctttcgaa gaggctgaaa ctgtcaaggg cggaatcatt
      661 atccctgaca ccgccaaaga gaaaccgatg cagggtgagg tcgtggaagt cggccccggc
      721 cgggtcaacg aggagggcaa gaagacgccc atggaagtca agaagggtga ccgcgtcctc
      781 tacggcaagt actccggcac cgaagttacg atcggcggca ccgagtacct catcatgcgc
      841 gaatccgaca tcttcgccat cgtccagggg taaaccacac gacacaagaa gagagaaagg
      901 ctgattccat atggcaaagc agattgacta caacaccgaa gcccgcgcca agctcaaaag
      961 aggcgttgac aaactggcca atgcggtcaa agtgacgctc ggccccaagg gccgcaacgt
     1021 cgtcatcgac aagaaattcg gctccccgac cgtcaccaag gacggcgtca cggtggccaa
     1081 ggagatcgag ctcgaagacc acttcgaaaa catcggcgcc cagatggtca aggaagtggc
     1141 gtccaagacc tccgacgacg ccggcgacgg cacgacgacg gccaccttga tcgcccaggc
     1201 gatctaccgg gaaggcctca agaacgtcac cgccggccac aatccgatgg cgatcaagcg
     1261 cggcatcgac cgcgccgtcg aagtcgtcgg cggcgagatc aagaagatgt ccaagccggt
     1321 ctcgggcaag caggagatcg cccaggtggg caccatctcc gccaactccg acaagcagat
     1381 cggcgacctc atcgccgagg ccatggagaa agtcggcaag gacggcgtca tcacggtcga
     1441 ggagtccaag tccgccgaca ccacgctcga ggtggtcgag gggatgcagt tcgaccgcgg
     1501 ctatgtctcc ccctactttg tgaccgaccc cgacaacatg gaagcggtcc tcgatgagcc
     1561 gctcatcctg atccacgaca agaaggtttc ctcgatgaag gatcttctgc cgatcctgga
     1621 gaaggtggcc caggccggcc ggccgctcat gatcatcgcc gaggacatcg agggcgaagc
     1681 gctcgccact ctcgtggtca acaagctgcg cggcacgatc aaggtgggcg cggccaaagc
     1741 ccccggcttc ggcgatcgcc gcaaggccat gctcgaagac atcgccgtcc tcaccggcgg
     1801 caaggtgatc agcgaagagg ccggtttcaa actcgagaac gccgtcatgt ccgacctcgg
     1861 ccatgccaag aaagtcaccc tcgacaagga caacaccacg atcgtcgagg gcggcggcca
     1921 gaaagaggcc atcaaggccc ggatcaacca gatccgcaag cagatcgagg acaccacgag
     1981 cgactacgac cgtgagaaac tccaggagcg gctggccaaa ctcgccggcg gcgtggccgt
     2041 catcaacgtc ggcgccgcca ccgagaccga gatgaaggag aagaaggccc gcgtcgagga
     2101 cgccctccac gccacccgcg ccgcggtcga ggaagggatc gtgcccggcg gcggcgtcgc
     2161 cctcctgcgc tgcatgaaag ccctcgacag cctgaaagtc accgaggaag agcaggtcgg
     2221 cgtcatgatc gtccgcaagg ctctcgaaga gccgatccgg cagatcgcca gcaacgccgg
     2281 cgccgaggcc tccgtcgtgg tcgacaaggt cctcgcacag accggcgcct tcggctacaa
     2341 cgcccagacc gacacctacg aggacctgct caaggccggc gtcatcgacc cgaccaaggt
     2401 cactcgcacc gccctcgaaa acgccgcttc catcgccgga ctcctcctca cgaccgaggc
     2461 ggtcgtggtc gagcagcccg aggagaagaa gccggccccc gctgcccccg acatgggcgg
     2521 catgggcggc atgtactaga ctgacccctc ctcttgtgat gcgaaagaag ccccggtcgc
     2581 cgcgaccggg gcttttgctt gcgcccgcat cgcacccggc cgcgcccgaa acatcgcccc
     2641 atcaacggaa aatactcttt ggcaattcct cccccttcga ccatatttct cccagcccca
     2701 ctcgactgcc gccgcctcgt tgcagggcga aagtgatcac cgtcgcggcc ggcagccctg
     2761 cgcgccgaga aggagaccca tgaccctcgc gaaaggccac aggctcggtc cctacgagat
     2821 cgagggcaaa accggctcgg gcggcatggg cgaggtctac cgggccaaag acacccgcct
     2881 cgaccgcacg gtcgccatca aggtcctccc cgaacactcc gccttaaacc ccgaggccag
     2941 agcccgtttc gaacgcgagg ccaggacggt ctcgagcctg aaccatccgc acatctgcgt
     3001 gctccatgat ctcggccggc aggaaggcgt tgacttcctg gtcatggagt atctcgaggg
     3061 cgaaacgctg gaggatcgcc tccgccgcgg ccggcttgac ctcagcgagg ttctgaccat
     3121 cgcctcccag gtcgccgccg ccctcgaggc cgcccaccgc aaagggctgg tccaccgcga
     3181 tctcaaaccc ggcaacatct tcctgactga agacggcgcc aaactccttg atttcggcct
     3241 cgccaaaccc caggctgagt ttgtccaggg gctggacggg gagactcgca ccacccctgt
     3301 caccggcgcg ggagccattg tcggaaccct gctgtacatg tcgcccgagc aactcgaggg
     3361 ccgcgaagcc gacgcccgct cggacatctt cgccctgggc gcgacgctct acgagatgat
     3421 cgccggtgag cgcgctttca ccggcgacag caaggccggc ctcatcggcg ccatcatgag
     3481 cgaacagccc cgcgccattg tcgaacggct gccgagcgcc ccgcccgtcc tcgaccgcct
     3541 ggtcaggaag tgcctgcaga agagccccga ccgccgctgg cagaccgccg gcgacctgaa
     3601 agacgagttg gactggatcg cctcggccgg ttcacaggct ggccttgccg ccccggtggc
     3661 gctccagcgg cgtttccgtc tgcgcctcgc atggatactg gccgcggtgt tcgccctgac
     3721 cgcggcgctc ctcggggccc aatggctgac gcagaaaacg cccgttgccc ggaccatgcg
     3781 cttccggatg ccgaagccgg agggcctggc gagaaccgac tggccgcagc tctcgcccga
     3841 cggccgctat ctgtcgctcc tggcgaccga taccagcggc gaaacgcaaa tctggatccg
     3901 acccctgagt tcaccccagc cgtacccgct ccccggaacc gccaatgccc gccgcccctt
     3961 ctggtcgccc gacggcgagt ttctggcctt cttcgatcgg tcctcggacc ggctcatgaa
     4021 ggtccccgtg gccggggggc aaccgcagat agtctgcaaa acggccggct ccgacggtac
     4081 ctggggccgc aacaacatca tcctgttcga cgattaccgc gactaccgat ccatcggcca
     4141 ggtgtcggca tcgggcgggg agccccggct ggccgtcgcc cctgacacgg cggccggaga
     4201 actgaccgcc acctggccgt gcttcctacc cgacgggcag cacttcatct tcacctgcgg
     4261 cagcgactca cttcgaggca cggatcaggt gtttctcaag ctcggggata ttgacggcgg
     4321 cgaaagccgt acactcggca ccgtggaatc ccgggcggtc ttctgcgaac cggagtacgt
     4381 gctctacatg aagaacggct ttttggtagc gcaccggttc aatctgaagc gtctggagct
     4441 ggaaggcgag ccggtcccgc ttaccgaccg tgtcgggatt tcagccggtt cgaacctggg
     4501 ggtgaatgtc gcggcctcgg ccaccggcat actggcctgg cagacgaagg gacaggcgaa
     4561 aagccgtatg gtctggaccg accgggccgg cgttgcgcag gatacggtgg gcccaccggc
     4621 aaattaccgg gagatccggc tgtctcccga caacaggcgg atagcctgca ccatcgccga
     4681 aacaggagtg gagttcacac gtctatgggt ggcggacgtc gcccggggat cggtctcaat
     4741 cctcacgtcc ggcgccggaa actggtttga tgcgaccccg ctgtggttct ccgacagccg
     4801 gtccctggtg tacctctccg tttcggcgac ggatctcacc gcgagtctgt gccgccaact
     4861 gatcagccgg agcgaggcct ccgtcctgcg gcagtcgacc ggggacggtc tcctcccgct
     4921 tggctggacg gcctccggaa acctgatcgt cggagccatc cccctgtccg ccgtctcgac
     4981 cgataccgcg tggaggctgg tggcgatcga tccggaagac actggccgcc tcgatacgct
     5041 tctcaccgga ggggacgcgg gcgttctcta cgatgtctcc cccgacggac gttacctgct
     5101 ggtcggaatg acctccgggg tcgagagcgg gttgcacgtg cgcgacctcc tccaacccga
     5161 caatgcctgg cttctgccgg ggtcggggac cgcggcccgt tggagcccgg cgggtgatga
     5221 gatattctac tttgaaggcg acaaccttgt cgcactggat atcgacctca ggggaggatt
     5281 cgccttcggc gctcccgaca ccctcttccg tcgccatttg gtcgccggac ggacgacact
     5341 ccgggggtat gacgtatcgt acgatggccg ccggttcctc ttcgtcgctc cggtcggccc
     5401 ggaagagcag gagccgaacg aaattgagat tgtcctcaac tggaacgccg aactgaagag
     5461 gaagtgacgc gcgccgcacg gcgtcggctc ggccccggac ggcgtgcgac cgccttggga
     5521 agcgcaagcc gagtcatctt ttctttcggc aatgcctgca ggagcctcag tcgccggaac
     5581 cgaggcccat cggcgggcgc gcctgtcggc agactctctc ccgcgcagac gtcaacatca
     5641 tgttgcgttc ggccgggtgt gtggtatctt gctgtaaacg taggaaacta ccgggatgaa
     5701 ccgcagggaa ttcttgaccg aggccgggcg cctagccacg ctggccatcg tcgccggtca
     5761 acctggactg atcagcctgg gctgtcagtc ggccgggtat cggcgagcct ccggtcccaa
     5821 acccgacttc ctcgtcccgc ccgatccccg tcttccgggc gtcgttcttg cccgcgacga
     5881 ggatcatgcg gcggcgctgg gcgccgccct cgaggggatc ggcggcattc ggcgcttcat
     5941 ccagccggga gaacgggtcc tcctcaaacc gaacgccgcc tgggatcgcg tgccggcgca
     6001 ggcggtcaac acgaatccgc tcgtggtggg ggagatggtg cgccagtgcc gccttgcagg
     6061 cgccgccgag gtcctcgtca ccgatcatgg cggcccgcga ccagaggagg ctttcacccg
     6121 ctccggcatc gccgccgccg tcgcacagaa cggcggccgg gtgatctatc ttgaggagga
     6181 cgattttctg gaggatgatc tgggcggccg cttcattggc gcctggccgg tcctgcgcca
     6241 cgttttcgag gtcgaccgct tcatcaacat gccgatcgcc aaacaccacg gcctcgtcgc
     6301 cggcaccgcc tcgatgaaga acttcttcgg cgctatcggc ggtgtccgcg acgtgctcca
     6361 cgatcagctc gaccaggcga ttgtcgatct ggccgcctac ttccggccta ccctcacggt
     6421 gatcgatgcg acccgcgtgc tcatgcggca cggcccgggg ggcggtgcgt tcgaggatgt
     6481 cgccgtccgc aatgcggtga tctgcgctac cgaccaggtc gccggtgact cctgcgccgc
     6541 cggtttcctc ggcgccgccg gccgcgacat tcgccatatc atcctcgccg cgcagcaggg
     6601 ccttggcgag atcgactacc gccgcgccgg gttcctcgaa ctgggcggca tctaggctct
     6661 tgagcgaaag tccgcgtgca atccgcggtc ggggggaaaa caatatgggt ggccaccttc
     6721 tccagggcag ggttgatcgc aatggctctt tctcttgcca ctacggcggt gggccaccca
     6781 tttgtcgcga acgtctcgac agtgagcgat cgcgctcctg cccgcgcgcc atcgctcacc
     6841 cccgcgaaac gtccgcccat caggcccagg cgatttcgac ggcgcctggt ccgccgcgcg
     6901 gccgggacgc cggccgccgc gtgtgtcggt ctccccggaa cccccccgct tcggcgctcc
     6961 cgctacctcc gcacgctcga aaaggcgttc attcccgggt actccgcata tttgccgatc
     7021 tgctcctcga tccggaggag ctggttgtac ttcgcgatcc ggtcggtccg cgacatcgac
     7081 cccgtcttga tctgccccgc gctggtcgcc accaccacgt ccgcgatcgt cgcgtcttcc
     7141 gtctcccccg accggtgcga cacgaccgcc gtataccccg cgtgctgcgc catgcggatc
     7201 gcgtcgagcg tctcagtcag cgttccgatc tggttcagct tgatgagaat cgaattggcg
     7261 atcccctccg cgatcccccg cccgagccgc ttgatgttcg tcacgaacag atcgtcgccg
     7321 accagctgga tccggctccc cagccgctcg gtcatctctt tccacccctt ccagtcgtcc
     7381 tccgccagcc cgtcctcgat cgagatgatc gggtacttgt cgcacaggcg ttcgtagaag
     7441 tcgatcattt cgctcgacga gagctttttc ccctccgcct tgaggttgta cttgcccgac
     7501 ttggcgtcgt agaactcggt cgaggccggg tcgagggcga tccggatctg ttcgccgggc
     7561 tggtagccgg ctttggcgat cgcctccatg atcacctgga gcgcttcctc gttggacttg
     7621 agatccggag cgaagccccc ttcgtccccc accgccgtgt tgtacttctt ggcggccagc
     7681 acttttttca ggtggtggaa cacctcggag cccatgcgga gcgcctcgct gagactcccc
     7741 gcgcccaccg gcatgatcat gaactcctgc aggtcgacat tgttgtccgc gtgcttgccc
     7801 ccgttgatga tgttcatcat cggcaccggc aggagcttgc agttgcagcc cccgatgtac
     7861 tcgtagagaa accggccgcg cgactcggcc gccgctttcg ccgtcgccag cgacacgccg
     7921 agcaaggcat tggccccgag cttccccttg ttctcggtcc cgtcgagccc aatcaggtag
     7981 ttgtcgaccg ccacctgatc gtacggatcg atcccgtctt tgagcagcgc cggggcgatc
     8041 ttctcgttga cgttgtcgac cgccttctgc acccctttgc cgagatactt gcttttatcg
//
LOCUS       DAOPOE010000119         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10105_length_7990_cov_4.154631, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000119 DAOPOE010000000
VERSION     DAOPOE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10105_length_7990_cov_4.154631"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /gene="eno"
                     /locus_tag="PKM94_12250"
     CDS             complement(<1..172)
                     /gene="eno"
                     /locus_tag="PKM94_12250"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256292.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD67446.1"
                     /translation="MSDYEFIHARQIFDSRGTPTVEVDVILSDGTLGRAAVPSGASTG
                     AHEAVELRDGDKS"
     gene            733..984
                     /locus_tag="PKM94_12255"
     CDS             733..984
                     /locus_tag="PKM94_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67447.1"
                     /translation="MATTPSSSAQPAEAAQPQTGEKPKISLPDLNWKTATCVVCGKPF
                     DYHGRKPKTCRDGNCRYRFEYKIEPDKWAGRQKTLFDVE"
     gene            complement(1268..1849)
                     /locus_tag="PKM94_12260"
     CDS             complement(1268..1849)
                     /locus_tag="PKM94_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOD67448.1"
                     /translation="MTHSLPDLPYAKDALAPHVSAETLEYHYGKHHLAYVTKLNELIR
                     GTQYEDASLEEIIRKSEGGIFNNGAQVWNHTFYWHCLSPAGGGEPKGKLAEALTRSFG
                     SFDKFKEQFTAAAVGLFGAGWVWLARKADGGLEIVPKGNAGNPLRDGQKPLLTCDVWE
                     HAYYIDYRNLRAKYLETFWKLVNWEFVGKQFSA"
     gene            2157..2414
                     /locus_tag="PKM94_12265"
     CDS             2157..2414
                     /locus_tag="PKM94_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67449.1"
                     /translation="MEIGLIYSKDDPRQTETRDFVHSYLRDRGILAQIVEDHQPVASP
                     TVVINGEAVRDRRAKPRDGHGRMFPALADIARAIEQHAWSL"
     gene            2703..4508
                     /locus_tag="PKM94_12270"
     CDS             2703..4508
                     /locus_tag="PKM94_12270"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOD67450.1"
                     /translation="MQMLLKAGLTIVAAALVLAGCGGDPMPTTPAAAPQAYKALAGVP
                     TTATIDSAWVAIHVSRPDTQTVAVHPVLGEWTETELTYAGRPAVDTLDTLAVFTVVDT
                     GWIMIPISGLAADWVEGRDNFGLIFRQADTAAGFVGWDSREAAGLEPYLRLCYSWKAD
                     TMCEVFTAVADAYIDEAAPDANFGAAAELFTGRDTAEGTSRAALVHVEIPVYTPPATL
                     GDFVWLDTDRNGVQDDGEPGLASITIDLFACDGDTLVASAATDLSGYYRFDSLAPGAY
                     YIAAALPDSLMYALPDQGGDETADSDLDPATGRSACVDLAPGDDYRALDAGYTLAYAA
                     VGDFVWNDMNENGLQDAGEPGLAGIAVNLFACDGRPVASAVTDSSGRYLFENLEPGDY
                     YLQLANPFGWIFTYAGVGGDDLIDSDVDRYYKTTTCFALSPGEEDYSRDAGLIETDGC
                     THGKGYWKNHAGFGPQPDEVTRLLLPIYLGTPEGTKTIAVADARTAFDILQQHTYGHP
                     SNGITKLYAHLLTAKLNIVNFANPEAVYPLIDEIDAFLSEYDWNDWEMLAPDRQTMVQ
                     RWKGRLEMYNGGNLGPGRCDGDAAYDDDGDSDGDY"
     gene            complement(4591..5940)
                     /locus_tag="PKM94_12275"
     CDS             complement(4591..5940)
                     /locus_tag="PKM94_12275"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOD67451.1"
                     /translation="MTTKSEQKKNDELMDKLVSLCKRRGFIYPSSEIYGGLSSCWDYG
                     PLGAELKRNLKTVWWNAMTHRRDDVEGLDAAIFMHPQVWHTSGHVTEFHDPMVDCKKC
                     KARFRADKLESSRCPLKPSKSPLECGGELTEARQFNLMFKTHMGPMEDSASEVFLRPE
                     TAQGIYVNFLNVKNSSRQKIPFGIAQIGKAFRNEITPGNFIFRTREFEQMEMQYFVAP
                     GEDEKWMEYWRAQRWQFYLDLGIREARLRWHQHGPGELAHYAKAAWDIEYEYPFGWQE
                     LEGVHNRTDFDLGRHMQATGKDLRYSDDVYTEKFVPYIIETSAGCDRTLLTLLVDAYD
                     EEEIKGETRVFLRLSPKVAPIKAAVFPLVKKDGMPEFAEKVYAELKRHFKVFYDVSGA
                     VGRRYARMDEAGCPFCITIDGQTIEDETMTLRFRDTQEQKRMKTAEVAAFLREQTQI"
     gene            complement(5964..6755)
                     /gene="recO"
                     /locus_tag="PKM94_12280"
     CDS             complement(5964..6755)
                     /gene="recO"
                     /locus_tag="PKM94_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOD67452.1"
                     /translation="MAIKATEAILLRAHNWSESSRTVVFFTRDYGRLALTDRGGRRLT
                     GKRGRLVPFARLELTFYHSEKESAGYLSDVSLVQAWQFEREGTLGRLAFASAAIELLL
                     SLLGDRAPHPDLYTYTVSYLELVETAPKQGLGPLFVNYYIRLISRLGYHPSLTRCVAC
                     GATGERLADAAGGVQFSAERGGMVCRACESVGDCYIPFSSESHRLIRALEGASLGQAA
                     TLPISYRETVRLTEALTRFLSFQADVRAELKALTFLEKLRKDAEA"
     gene            7032..7526
                     /locus_tag="PKM94_12285"
     CDS             7032..7526
                     /locus_tag="PKM94_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HOD67453.1"
                     /translation="MQTEPHPEHIWLQRFVGDWTIEIQAVMGPGEPPTTHTGRETVRS
                     LGGLWIVAEGHGETPGGSFDSILTLGFDPQRRRFVGSFIASVMTHFWIYEGALDAAGK
                     VLTLDCEGPVFGAEGKTTRYQDVHTWVSENERTLTGKILGPDGQWQDMMTSRYRRVRR
                     TATQ"
     gene            complement(7520..7699)
                     /locus_tag="PKM94_12290"
     CDS             complement(7520..7699)
                     /locus_tag="PKM94_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67454.1"
                     /translation="MAVHSARSRVQEETIVGELLQLIIDSMPKDVAAAAGKSTVEVDR
                     IEDARSPHRGRSFVY"
BASE COUNT         1402 a         2754 c         2372 g         1462 t
ORIGIN      
        1 tgcttttatc gccatcgcgc agttcgaccg cttcgtgcgc cccggtcgag gcccccgacg
       61 gcacggcggc ccgcccgagc gtgccgtccg aaaggatcac atcgacctcc accgtgggcg
      121 tgccgcgtga gtcgaaaatc tgtcgcgcat gaatgaattc gtagtccgac atggagaaac
      181 ctctcgtcag ccaattgccg tttcacatcc gccgtagatg ctaccatccg ccccggacgc
      241 atgcaagttt taacagtcat tggggggaca agccctcaga gtatggggcc cggccgcgac
      301 ttccgcgctg tggccggtcc cgatcccttt gtgcggcaac acgcttggcg ccggcacccg
      361 gtttgatacg taggccgtgt accttgggta ggacagcaag accggcggat taaaagatgc
      421 cggtcggggc cggccgctaa ccggcccctt ttttattccc tcaccaaatg tgattgtgct
      481 ctcccccgcc acgggtagtt tctggcttag cgcggcccga aacagccggc ggatggcggt
      541 tttcgactcg cgctaaagaa aaaactcgac aaaccggtgc aaaagcgcgt taaacccaat
      601 ctgaacacga ggccgacgcg agccgcctgc ccccgcggga aacggtcgcc ggccgcggaa
      661 gcgaaaaccc gaccggcgtc cgattggcca gagcggccgg ggcgcccgag gattgacaac
      721 ccgagagaca ctatggctac cactccttcg tcctccgctc agccggccga ggctgcccaa
      781 ccgcagaccg gcgaaaaacc gaaaatctcc ctccccgacc tgaactggaa gaccgctacc
      841 tgcgtcgtgt gcgggaaacc gttcgactat cacggacgga aaccgaaaac ttgccgcgac
      901 ggcaactgcc gctaccgttt cgagtacaaa attgagcccg acaagtgggc cgggcgccag
      961 aaaactctgt tcgacgtcga gtagcccggc cggttcagcc tcccgtgcgt tctgcagcgc
     1021 ttcgcggcga gcaaaccccg gcgtataagg ggcaactcat ttccgaaagc gaggtgcccc
     1081 gggcctacct tactcggcct attcagcgcg ctcgcccttg caacggccgc ctttgcctca
     1141 tctttggcaa tgatctctac cgggagtgca gcctcggccg gctgacaccc ggagagagcg
     1201 accgccccct cccgcgccct tcgccccggc tggccgcgcc ccacaccacc cccgcctctg
     1261 cgccgctcta cgccgaaaac tgctttccga caaactccca gttcaccagt ttccagaatg
     1321 tttccaggta tttcgcccgg aggttgcggt agtcgatgta ataggcgtgt tcccagacgt
     1381 cgcacgtcag cagcggcttc tgaccgtccc gcagcggatt ccccgcattt cccttcggca
     1441 cgatctccag tcccccgtcg gctttcctcg ccagccagac ccagcccgcg ccaaacagcc
     1501 cgaccgcggc cgctgtgaac tgctccttga atttgtcgaa cgacccgaac gaccgcgtga
     1561 gcgcttcggc gagcttgccc ttcggttccc cgccgcccgc gggcgacaga cagtgccagt
     1621 agaacgtgtg gttccacacc tgggcgccgt tgttgaagat ccccccctcc gatttccgga
     1681 taatctcctc cagcgaggcg tcctcgtact gagttccccg gatgagctca ttgagcttgg
     1741 tgacgtaagc caggtggtgt ttgccgtagt gatactccag cgtttcggcg gaaacatgcg
     1801 gcgcgagcgc atcttttgcg tacggcaggt cgggcagcga atgggtcatg ggaggctcct
     1861 ttccccggac cggcgattga gccgtcatta ggtttgcccg aacgttacca ctctgtcatg
     1921 gcggtgtcaa gcggaggccg acgttggtcc cgccgcagat aattgtcaag ttcggtcgcc
     1981 gcgtctccga taatctgagt gaagactgat ggtgacttaa cggaaatcgg acgttttgtt
     2041 cgtttattgg gaaaccagtc gtgcggttgg ttcgttaaag agacaaagag ctttttagtt
     2101 gaccgcatcc tggcgtccta acactttaca tctggttact catggggtga aaggccatgg
     2161 agattggctt gatttattca aaggatgacc ctcgccagac tgagacgcgg gattttgtgc
     2221 actcgtacct tcgagatcgg ggaatcctgg cccagattgt cgaggaccat caaccggtcg
     2281 cttccccgac cgtggtcatc aacggcgagg ctgttcgaga ccgccgcgcc aagccgcgtg
     2341 atggccatgg ccggatgttt ccggccctcg ccgacatcgc ccgggccatc gagcaacacg
     2401 cgtggtcttt gtaacgggtt agcagaaggt actcgaccgc acattttgtc agtttacggc
     2461 gcggcccccc ggccgcgccg ttctcgtgtc cggccggcgc ccggcccgaa aacccgcgtc
     2521 cggcgcggga tttcccagaa aacgctttac tttgtcccgc cccgccgata ctataacggg
     2581 gtcaacccac tgttactgca acgacctttg cctgtacctg caagcgggct gagaaaactc
     2641 aacgattcgt gtatttcccg ggttgctccg tgcgcccgga cgtgcgaaag gagacagatc
     2701 ctatgcaaat gctgctgaaa gcaggattga ccatcgtcgc cgcggccctg gtcctggccg
     2761 ggtgcggcgg cgacccgatg cccaccaccc cggccgccgc tccccaggcg tacaaggctc
     2821 ttgccggcgt gcccaccacc gccaccatcg actccgcctg ggtcgccatc cacgtcagcc
     2881 ggcctgacac ccagacggtc gcggttcacc ccgttctggg cgagtggacc gagaccgaac
     2941 tcacctacgc cggccgcccg gccgtcgata cacttgatac cctcgcggtc ttcactgtcg
     3001 tcgacaccgg ctggatcatg atcccgatca gcggcctggc agccgactgg gtcgagggtc
     3061 gggacaattt cggcctcatt ttccggcaag cggacacggc ggcgggcttc gtcggctggg
     3121 acagccgcga ggcggccggc cttgaacctt acctgcgcct ctgctatagc tggaaggccg
     3181 acacgatgtg cgaggttttc acggcggtcg ccgacgccta catcgacgag gccgccccgg
     3241 atgcgaactt cggcgccgcc gccgagctgt tcaccggccg cgacactgcc gaaggcacct
     3301 cgcgcgcggc tttggtgcac gttgagatcc cggtgtacac gccgccggct acgctcggcg
     3361 acttcgtctg gctggacacc gaccgcaacg gcgttcagga cgacggcgaa ccgggcctgg
     3421 ccagcatcac gattgatctg ttcgcgtgcg acggcgacac gctggtcgct tcggccgcca
     3481 ccgacctctc cggttactac cgcttcgatt ccctggcccc cggcgcctac tacatcgccg
     3541 cggccctgcc ggacagcctc atgtacgccc tcccggatca gggcggggac gagaccgccg
     3601 acagcgatct tgacccggcg accggccgga gcgcctgcgt ggatctggcc cccggcgacg
     3661 actaccgggc gctcgacgcc ggctacaccc tcgcctatgc cgcggtcggc gacttcgttt
     3721 ggaatgacat gaacgagaac ggcctccagg atgccggcga gcctgggctg gcgggcatcg
     3781 ccgtcaacct gttcgcctgc gacggccgcc ctgtcgcctc cgccgtcacc gactcttccg
     3841 gccggtacct cttcgaaaac ctggagcccg gcgactacta cctgcagctt gccaatccct
     3901 tcggctggat cttcacctat gccggcgtcg gcggcgacga cctgatcgac agcgacgtcg
     3961 accggtacta caagaccacg acctgcttcg ccctctctcc cggcgaggag gattactccc
     4021 gcgatgccgg tttgatcgag accgacggct gcacgcacgg gaaggggtac tggaagaacc
     4081 acgccggctt cgggccgcaa cccgacgagg tgacccggct tctcctgccg atctacctcg
     4141 ggacgccgga gggcactaag acgatcgcgg tggccgacgc ccgcactgct ttcgacatcc
     4201 tccagcagca cacctatggc catccgagca acgggatcac gaagctctac gcccacctgc
     4261 tcaccgccaa gctgaatatc gtcaacttcg ccaaccccga ggcggtgtac ccgttgatcg
     4321 acgagatcga cgccttcctg tcggagtacg actggaacga ctgggagatg ctcgctccgg
     4381 accggcagac catggtccag aggtggaagg gccgtcttga gatgtacaac ggggggaacc
     4441 tcggcccggg tcgctgcgat ggcgatgcgg cgtacgatga tgacggcgac agcgacggcg
     4501 attactgaga cccgctcccg ctgatcagcc aagacagaaa ggcccggcgc ttgcgcaccg
     4561 ggccttcctc gtggcttgtc cggcccgcgg ctagatctga gtctgctccc gcaggaacgc
     4621 cgcgacctcc gccgtcttca tccgcttctg ctcctgggta tcgcggaagc gcagcgtcat
     4681 cgtctcgtcc tcgattgtct ggccgtcgat cgtgatgcag aacgggcagc ccgcctcatc
     4741 catgcgcgca taccggcggc ccacggcgcc ggagacatcg tagaacacct tgaaatgccg
     4801 cttcagttcg gcgtacacct tctcggcgaa ctccggcatt ccgtccttct tgaccagcgg
     4861 gaacacggcc gccttgatcg gcgccacctt cggcgagagg cgcaaaaaca cccgcgtctc
     4921 ccctttgatt tcttcctcgt cgtacgcgtc gaccaggagc gtgaggagcg ttcggtcgca
     4981 cccggccgag gtctcgataa tgtacgggac gaacttctcg gtgtagacgt cgtcgctgta
     5041 gcgcaggtct ttgccggtgg cctgcatgtg ccgcccgagg tcgaagtcgg tccggttgtg
     5101 cacgccctcg agttcctgcc agccgaaggg gtactcgtac tcgatatccc aggccgcctt
     5161 ggcgtagtgc gccagttccc cggggccgtg ctggtgccag cgcagccggg cctcccggat
     5221 ccccaggtcg agatagaact gccagcgctg cgcccgccag tactccatcc acttctcatc
     5281 ttcgcccggc gcgacgaaat actgcatctc catctgctcg aactcgcgcg tgcggaaaat
     5341 gaaatttccc ggggtgattt cgttgcggaa cgccttcccg atctgggcga tcccgaacgg
     5401 gattttctgg cgcgaggagt tcttgacgtt gaggaaattg acatagatcc cctgcgccgt
     5461 ttccggccgg aggaacacct cgctcgcgga atcctccatc ggccccatgt gcgtcttgaa
     5521 catgaggttg aactggcgcg cctcggtcag ttctccgccg cactccaggg gcgacttcga
     5581 cggtttcagg ggacagcgcg aggattcaag cttgtcggcc cgaaaacgcg ccttgcactt
     5641 cttgcagtcc accatgggat cgtggaattc ggtcacgtgc cccgaggtat gccacacctg
     5701 cgggtgcatg aagatcgcgg catcgagccc ctcgacatcg tcccgccggt gcgtcatcgc
     5761 gttccaccac accgtcttga gattccgctt cagctcggca ccgaggggac cgtagtccca
     5821 gcacgacgac agcccgccgt agatctcgct ggaggggtag atgaagccgc gccgcttgca
     5881 cagcgacacc agcttgtcca tcaactcgtc attcttcttc tgttcgctct tggtcgtcat
     5941 accgtcttcg tgctttcctt tcctcaggct tcggcgtcct tcctgagctt ctccaaaaaa
     6001 gtcagcgctt tcagctccgc ccgcacatcc gcctggaagc tcaggaacct ggtcagcgct
     6061 tctgtcagcc ggaccgtctc acggtaactt atcggaagcg tggccgcctg tcccaacgac
     6121 gctccctcca gcgcccggat cagccggtgg ctctccgaag aaaagggaat atagcaatct
     6181 ccgaccgatt cgcaagcccg gcagaccatt cccccacgct cggccgagaa ctgcaccccc
     6241 ccggcggcgt cggccagccg ctcccccgtc gccccgcacg ccacgcaccg ggtgagcgag
     6301 gggtggtacc cgagccgcga gatcagccgg atgtagtaat tcacgaagag cggccccagc
     6361 ccttgtttcg gcgccgtctc caccaattcg aggtaggaaa ccgtgtaggt atagaggtcc
     6421 gggtgcggcg cccggtcccc cagcagactc agcagcagtt cgatcgccgc cgaggcgaaa
     6481 gccagccgcc cgagcgtccc ctcccgctcg aactgccacg cctgtacgag gctgacgtcc
     6541 gacaggtacc ccgccgattc cttctccgag tggtagaacg tcagctccag gcgggcgaac
     6601 ggaacgagcc gcccccgttt gccggtcagc cgccgcccgc cccggtcggt cagagccagc
     6661 cgcccgtaat cgcgggtgaa aaacaccacc gtccgcgagg actccgacca gttgtgcgcg
     6721 cgcaggagaa tcgcttccgt cgcctttatc gccatggtct caccgcagtt cccgttttga
     6781 gtgcgggtcg gcgccgcccc tatgccggcg gcccggaccg cgccccgcaa taacggcatt
     6841 ttgcggctgc tgcgccaccg ctaagcgctc tgccccccgc cggctgcgtt cgaaaggctt
     6901 ccgggccgcg accggcccgg tccgggcgag tgaacatggg tcaaaaaggt tcacaatgtg
     6961 attgcgcccg gccgcccgag ggcctatctt gccctcaccg gacagtaaac ctcttgaaat
     7021 gggagatcgc tatgcagacc gaaccgcacc cggaacacat ctggctgcag cgctttgtcg
     7081 gcgactggac cattgagatc caggcggtga tgggacccgg cgaaccgccg accacccaca
     7141 ccgggcgcga aaccgtccgc tccctcggcg ggctgtggat tgtcgccgaa ggccacgggg
     7201 agacgcccgg cggctcgttc gactccatcc tcacgctcgg ctttgacccg cagcgccggc
     7261 ggttcgtcgg cagcttcatt gcctccgtaa tgacccactt ctggatatac gagggcgccc
     7321 tcgatgccgc cgggaaagtg ctcaccctcg actgcgaggg ccccgtcttc ggcgccgaag
     7381 gaaagaccac ccggtaccaa gacgtccaca cctgggtctc cgagaatgag cgcaccctca
     7441 ccggaaagat cctcggaccc gatgggcagt ggcaggacat gatgaccagc cggtatcggc
     7501 gggttcgccg gactgcgact cagtagacga aggacctgcc tcgatgaggc gaccgggcgt
     7561 cttcaatccg gtcaacctcg acggttgatt tcccggctgc ggcggcgaca tctttcggca
     7621 tgctgtcgat gatgagttgc agcagttcgc cgacgatcgt ctcctcctgt acccgcgaac
     7681 gggccgagtg aaccgccatg cgcctcgtgg aagatgatcc cgccggaatt gcccgcgaac
     7741 tcaaacggca ggcgggggcc ggcctgcggg cgaggaacct gaccgcccac ctccggagcc
     7801 ctgaccgcct gtgtctcccg ggggatgaaa cggcgcaaag cgagacctcc cgcctcccgc
     7861 gctgcggaga ccttggcggg cggtctctgt cgtttgtgca cgtgtggggg gaatcccccg
     7921 cccccgtcac tggccgcgat accggtagat cacctcgttg ggccgtacca tgcggtagcg
     7981 tgtgcgggcg
//
LOCUS       DAOPOE010000120         7951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10180_length_7951_cov_4.963273, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000120 DAOPOE010000000
VERSION     DAOPOE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_7951_cov_4.963273"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PKM94_12295"
     CDS             complement(<1..149)
                     /locus_tag="PKM94_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOD67455.1"
                     /translation="MIERYTLPEMGALWTDEARFRTWLEVEVEAARAMAHYRVIPAKA
                     FATIA"
     gene            complement(155..799)
                     /gene="fsa"
                     /locus_tag="PKM94_12300"
     CDS             complement(155..799)
                     /gene="fsa"
                     /locus_tag="PKM94_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOD67456.1"
                     /translation="MKFFIDTANIAQIKKAHAMGVLDGVTTNPSLAAKEAAPYRELLA
                     EICRIVPGPVSAEVIATDYDGMMAEARELAKIADNIVVKIPTILDGLRAIKSLKEEGI
                     KTNATLIFSPSQALLVAKAGATYASPFIGRLDDISQDGMELIEQIVAIYANYLFETEV
                     LVASVRHPMHVVQAALIGAGVVTMPYDVIEKLIRHPLTDLGLAKFLADYKKAGK"
     gene            complement(812..1669)
                     /gene="truA"
                     /locus_tag="PKM94_12305"
     CDS             complement(812..1669)
                     /gene="truA"
                     /locus_tag="PKM94_12305"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOD67457.1"
                     /translation="MAERNIRLTLEYKGTAYAGWQIQAGQPTVQGELVAAIRKTTGCD
                     VSVHGAGRTDAGVHALGQVANFRIDHRLAPDRYRDALNYYLPADIRIREAAEAPLSFH
                     ARRSAKAKRYRYVIAGERSALWREYRWELAGREAAGASPEAAGVLPPADSLAGPGPGD
                     SPAAPAPALRFDLLQQAAALIVGEHDFAPFCVTASRKEDNRCRIDRAHWYRVGPLRVF
                     EIRGNRFLHNMVRSLVGAMVNLAAESPDRHPDNLTLTRFADIIQAPNQQRIVFTAPAR
                     GLYLVSVHY"
     gene            complement(1753..2556)
                     /locus_tag="PKM94_12310"
     CDS             complement(1753..2556)
                     /locus_tag="PKM94_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOD67458.1"
                     /translation="MFHAAPILLGQYRPVDSYLHKLDARAKMLPITVVLVFGLLSDSF
                     MFYAVILVALVAALLGSGVRAGQLVRNFKPIILLVVITAAYHLAFSGGRSEIWWAAGP
                     IRIREEAVRQAGFFSLRLVLFVSIAFLLTLTTAPSELADAIAQLLRPLERIRVPVQDL
                     ALILFMAMRFIPVLYDEFQAIKYAQMIRGVRFTGSMINRIRKTVSIIIPVFVAALQRA
                     DDLALAIEARGYKGGVKRTIYTRLRFETPEWVFALGASGAVVILWALTR"
     gene            complement(2826..2987)
                     /locus_tag="PKM94_12315"
     CDS             complement(2826..2987)
                     /locus_tag="PKM94_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67459.1"
                     /translation="MDMLWQHVAVGAALVGAAAYLGWYFRRRAFRKKSPCDSCGLMKM
                     AERRAGRGR"
     gene            complement(2987..5158)
                     /gene="feoB"
                     /locus_tag="PKM94_12320"
     CDS             complement(2987..5158)
                     /gene="feoB"
                     /locus_tag="PKM94_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOD67460.1"
                     /translation="MPAPEITKTREIAICGNPNCGKTTIFNAITGLAQQVGNYPGVTV
                     ERIRGQFAHGGRRYVLADIPGTYSLSAFSPDEYIACAALTGSLDASPAPDAIICVLDG
                     TQLERGLYFLLQVLEIGRPTVAAVNMVDIAAKRGLTIDYAALERLTGVPVVPVVGSRG
                     EGIARLVALAAQAADAPPLPPRRRYRAEVENLIERLQATPGNGHRSRAEYLRIVFDAD
                     GPAERRFLAAAGGAGRALLERGRSLIRERAGTLAAAETAPLTEAAAELYRQAVRAAAS
                     PRPSRSGRIDRFLLHPVLGPIVLVTAMMFVFQSIFSWAEPFMDLIDGLFGRLAAAVES
                     ALPAGPLQSLLTDGVIGGVGSVLVFIPQIVILFLFIALLEDSGYMARAAFLVDRMFRW
                     CGLSGKSFIPMLSSFACAVPGILATRTIEDRKLRFITMMVAPLMTCSARLPVYAVMIA
                     AFIPYKTWLGVINLQGLVLTVLYLLGIAVAVAVSFVLQRLVFRAERGTFLMEMPSYKV
                     PTLRSLGIRVIHRARSFVVRAGTVILAITILIWALSYFPRDSRLAESYAERIETAATA
                     AEAQTLANERAGAQLRNSYFARIGHAVEPVFRPLGWDWRITMATLAAFPAREVIIATL
                     GTIFNLGAEVDEESSSLIEKMRQAAWEYGPEAGRPLFTPAVALSIMVFFALCCQCGAT
                     LVTIRKETASWRYPLAVFGYMTALSYVTALATYQVFSRVWY"
     gene            complement(5203..5433)
                     /locus_tag="PKM94_12325"
     CDS             complement(5203..5433)
                     /locus_tag="PKM94_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOD67461.1"
                     /translation="MTYLSRMSPGQRGRIVGFVDDSPLARRLLELGIIPGRLITYLRN
                     APLSDPMQVQVGSSCLTLRHQEASLVAVEPEE"
     gene            complement(5464..5916)
                     /locus_tag="PKM94_12330"
     CDS             complement(5464..5916)
                     /locus_tag="PKM94_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOD67462.1"
                     /translation="MREFLRTKGFKMTPQRELVFRAFFELGDHVTVDELFEKVRRQDR
                     SIGYSTVWRNLKLICRVGLAEEVNVGDGVTRYDRVTHVPHGHLYCQECRRLTEFPVEE
                     IVAVLAGVAEQNDFRADSFKIEIHGYCEECRERRRRALGSGGGAAPPK"
     gene            complement(6120..>7951)
                     /locus_tag="PKM94_12335"
     CDS             complement(6120..>7951)
                     /locus_tag="PKM94_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD67463.1"
                     /translation="IDVTQNIKLAAIPAVEYNPVVYRLRNATTGSSEYWLIENRRQTG
                     FDTNIPGSGLCIWHYDAGTAAGTGSNRYPPYRIALEQADGQFQLEYAGSSGDAGDPFP
                     GATGNRNFHDYSTPATNLNDDLSPLYGLVSRIGVWDVSNADSVMYAELDIEYSRPWIV
                     EASSGFVFNDDAPRGDGDGVVEAGEEIHFYCYIRNFMRYSYNVRAALASGSPRIEWVT
                     PAVSFGTNLLVDIQYNTDPIIFRVKDSLTAAIDTFTVTIVTDSLPGGTFGSEFVKEFQ
                     FSKQVGATQVLVVDDDNGAEYQQEYVDALRGRGIPSRVWNVQSQGSPDSLALSGYRIV
                     FWHTGIDTSTGAFSAANVAAMKGYLNAPDHHLCLGTYALVKLAALDNAFVADYLKASH
                     AGNLYFPQYLGVPGTAIGNGTEYRPITGAPNAAAANAYMNVLSGGQSIFTGGPSGQIR
                     GIAYAGPTYKTVLLTFAPELLNDNAGGTTMPKDTLISRILAYFGGQATSIHDGSPFPV
                     MPSSFTLEQNYPNPFNPSTTIRYTLRSTGADFRSTPRTKLAVYNALGQRVATLVDEVQ
                     IPGSYAVDWDGSDGRGHRAASGVYFYRLERGDEAQTMKMVLLK"
BASE COUNT         1391 a         2687 c         2546 g         1327 t
ORIGIN      
        1 tcggcgatcg tcgcaaatgc cttcgccggg atcacccggt agtgggccat ggcccgcgcc
       61 gcctccacct cgacctccag ccaggtgcga aaccgggcct cgtcggtcca cagcgccccc
      121 atctccggaa gagtgtaccg ttcaatcatg ggcgttactt gccggctttc ttgtagtcgg
      181 cgagaaattt cgccagtccg aggtcggtga gcgggtggcg gatcagcttc tcgatcacgt
      241 cgtagggcat cgtcaccacc ccggccccga tcagcgccgc ctgcaccacg tgcatcgggt
      301 ggcggaccga ggccaccagc acctcggtct cgaacaggta gttcgcgtag atggctacga
      361 tctgctcgat cagttccatc ccgtcctgcg agatgtcgtc gagccgcccg atgaagggcg
      421 aggcgtaggt cgcccccgcc ttggccacca gcagcgcctg cgagggggag aaaatgagcg
      481 tcgcgttcgt cttgatcccc tcctccttca ggctcttgat cgcgcggaga ccgtcgagaa
      541 tggtcgggat tttcaccacg atgttgtcgg cgattttcgc cagttcgcgg gcctccgcca
      601 tcatgccgtc gtaatccgtg gcgatcactt cggccgacac cggcccgggc acgatccggc
      661 agatctccgc gagaagctca cggtaaggcg cggcttcttt ggccgccagc gaggggttgg
      721 tggtgacgcc gtcgagcacg cccatggcgt gcgccttctt gatctgcgcg atattggcgg
      781 tgtcgatgaa aaacttcatc tgatcatccc ttcagtagtg taccgacacg aggtacagcc
      841 cccgggccgg cgccgtgaac acgatgcgct gctggttcgg agcttgtatg atatcggcaa
      901 accgggtcaa agtcaagttg tccgggtggc ggtcggggga ctcggcggcg aggttcacca
      961 tggcccccac aagactccgt accatattgt gcaggaaccg gttcccgcga atctcaaaca
     1021 cccgcagcgg gccgacccgg taccagtgcg cccggtcgat ccggcaccgg ttatcctcct
     1081 tgcgggaggc ggtcacgcag aacggggcga aatcgtgctc gccgacaatg agcgccgccg
     1141 cctgctgcag gagatcgaaa cgcaacgcgg gcgccggagc ggccgggctg tcccccggcc
     1201 cggggccggc aagactatcg gccggcggta agacgcccgc cgcttcagga gaggccccgg
     1261 ccgcctctcg ccccgcgagt tcccaccggt actcccgcca cagcgccgag cgttcccccg
     1321 cgatcacgta gcggtaccgc ttggccttgg cgctccggcg ggcgtggaac gacaggggcg
     1381 cctcggccgc ctcccggatg cggatatccg cggggaggta gtaattgagg gcgtccctgt
     1441 agcggtccgg cgccaggcgg tggtcgatgc ggaaattggc gacctggccg agcgcgtgca
     1501 cgccggcatc ggtccgcccc gcgccgtgaa cgctcacgtc gcagcccgtt gtcttgcgga
     1561 ttgcggcaac tagttccccc tgcacggtcg gctgccccgc ctggatctgc caccccgcgt
     1621 acgcggtccc cttgtactcg agcgtgagtc tgatgttgcg ttcagccatt cgctatccgt
     1681 cgccgcacag aaccggctcg aggcggcggg ctccgggtcg cgccccgctt cctgccgccc
     1741 gccctcttgc gctcatctcg tcagcgccca caagatcacc accgcgccgc tcgcgccaag
     1801 cgcgaacacc cactccggcg tctcaaaccg caaccgtgta tagatcgtcc tcttcactcc
     1861 gcccttgtac ccgcgcgcct cgatggcgag cgccaggtcg tccgcccgct ggagcgccgc
     1921 cacaaagacc gggatgatga tgctgacagt cttgcggatg cggtttatca ttgagcccgt
     1981 gaaccgcacg ccccggatca tctgggcgta cttgatggcc tgaaattcgt catagaggac
     2041 cgggatgaac cgcatagcca tgaacaggat gagtgcgaga tcctgcaccg gcacgcggat
     2101 ccgttcgaga ggccggagca gctgcgcgat agcgtcggcg agttcggagg gggcggtcgt
     2161 gagcgtcaac agaaacgcaa tcgacacaaa aagcaccagc cgcagcgaga agaatcccgc
     2221 ctggcgcacc gcttcctccc ggatccggat cggcccggcc gcccaccaga tctccgaccg
     2281 gccgccggag aaggccaggt ggtaggccgc ggtgatcacc accagcagaa tgatcggttt
     2341 gaaattccgc accaactgcc ccgcccgcac ccccgagccg agcagcgccg cgaccagcgc
     2401 cacgaggatc acggcgtaga acatgaatga gtcggacagc aggccgaata cgaggaccac
     2461 ggtgatcggg agcatcttgg cccgggcgtc gagcttgtgc aggtaggaat cgaccggacg
     2521 gtattgcccc aaaagtatcg gcgccgcgtg aaacatgggg ggaatatagg gggatcggaa
     2581 gaaccaggtc aatcagtcat tcgagcgcct tccgctccga tggccggttg ccccgcgttg
     2641 ctgacaatcc ggtttgacgg aagtcccggt tgccggagcg cacggccgcg aggtgcagca
     2701 cagcgcggcg gcccttcacg cttacctggc cctcgcccgg agggaacacg ggcgccgggt
     2761 cgatttcccg cgcctccccg aatccgtgcg gcgaccgccc ctcctgcgcg cggctgctcc
     2821 gaggcctacc tgccccgccc cgcccgccgc tccgccatct tcatcaaacc gcatgagtcg
     2881 cacggcgact tcttccggaa cgccctccgc cggaaatacc acccgaggta ggccgccgcc
     2941 cccaccagcg ccgcccccac cgcgacatgt tgccacagca tatccattag taccacaccc
     3001 ggctaaacac ctgataggtc gccagcgccg tcacatacga cagcgccgtc atgtagccga
     3061 acaccgccag cgggtaccgc cacgaagcgg tttccttgcg gatcgtcacg agcgtcgccc
     3121 cgcactggca gcacagggcg aagaacacca tgatcgacag cgccacggcc ggtgtaaaga
     3181 gcggccggcc ggcctccggg ccgtactccc atgccgcctg ccgcatcttc tcgatgagcg
     3241 aggacgactc ctcgtccacc tccgccccga gattaaaaat cgtcccgagc gtcgcgatga
     3301 tcacctcccg ggccgggaaa gccgccaggg tcgccatcgt gatccgccaa tcccacccga
     3361 gcggccggaa caccggttcg acggcgtgcc cgatgcgggc gaagtaggaa ttgcgcagct
     3421 gcgcgccggc ccgctcgttc gccagcgtct gcgcctcggc cgcggtcgcc gccgtctcga
     3481 tcctctccgc gtagctctcg gcgaggcggg agtcgcgcgg gaaatacgac agcgcccaga
     3541 tcaggatcgt gatcgccagg atcaccgtcc ccgcccgcac cacgaacgac ctcgcccggt
     3601 ggattacgcg gatgccgagc gagcggagag tcgggacttt gtacgacggc atctccatga
     3661 ggaaggtccc gcgctcggcc cggaacacga gccgctggag gacgaacgag accgcgacgg
     3721 cgaccgcgat cccgagcagg tagagcacgg tgagcaccag gccctgaagg ttgatcaccc
     3781 cgagccaggt cttgtagggg atgaaggccg cgatcatcac cgcgtacacg ggcaggcggg
     3841 ccgagcaggt catgaggggc gccaccatca ttgtgatgaa gcggagtttg cggtcctcga
     3901 tcgtgcgggt ggcgagaata ccgggcacgg cgcaggcgaa cgaggagagc atggggatga
     3961 acgacttgcc cgacagcccg caccagcgga acatgcggtc caccaggaag gccgcccgcg
     4021 ccatgtagcc gctgtcctca agcagagcga tgaacaggaa gagaatgaca atctggggga
     4081 tgaaaacgag caccgaaccg acgccgccga tcacgccgtc ggtgagcagc gactggagcg
     4141 gcccggccgg cagggcgctc tcgacggccg cggccagccg tccgaaaagc ccgtcgatca
     4201 ggtccatgaa cggctccgcc caggagaaga tcgactggaa cacgaacatc atcgccgtca
     4261 ccagcacgat cggtccgagg acggggtgga ggagaaaccg gtcgatccgc cccgagcgcg
     4321 agggccgagg gctggcggcg gcccgcaccg cctggcggta gagctccgcg gccgcctccg
     4381 tcagcggtgc ggtctcggcc gccgcaagcg tgccggcccg ctcgcgaatc agactgcgcc
     4441 cgcgctcgag caacgcccga cccgcgccgc cggcggcggc caggaatcgc cgctccgccg
     4501 gtccgtcggc atcaaacacg atccgcaaat actcggcgcg cgaccggtgg ccgttgcccg
     4561 gcgtcgcctg caggcgctcg atcagattct ccacttccgc ccggtagcgc cgccgcggcg
     4621 ggagcggcgg ggcgtccgcc gcctgcgcgg ccaacgcaac cagccgcgcg atgccttccc
     4681 cccggctgcc gaccaccggc acgaccggca cgccggtcag ccgctccagc gcggcgtagt
     4741 cgatcgtcag cccccgcttc gcggcgatat ccaccatgtt gaccgccgcc acggtcggac
     4801 ggccgatctc cagcacctgg agcaggaaat agagcccccg ctcgagctgg gtcccgtcga
     4861 gcacgcagat gatcgcatcg ggcgccggcg aggcatccag gctgcccgtg agtgcggcgc
     4921 aggcgatgta ttcatcggga gaaaacgccg agagggaata ggtgcccggg atgtcggcca
     4981 gcacgtaccg ccggccgcca tgggcgaact gcccgcggat ccgctcgacc gtcacgccag
     5041 ggtagttgcc cacctgctgg gcgagaccgg tgatggcgtt gaagatcgtc gtcttgccgc
     5101 aattcggatt gccgcaaatg gcgatttcac gggtctttgt aatctcgggc gccggcatgc
     5161 gacctccccg aaggatggcc tctcccttgg gaaatggcgg ggttactcct ccggctccac
     5221 tgccaccagc gaggcttcct ggtgccgcag ggtcaggcag ctcgaaccga cctgcacctg
     5281 catgggatcg gagagcggcg cgttccgcag gtaggtgatc aggcgcccgg ggataatccc
     5341 cagctccagc aggcggcgcg ccagtgggct gtcgtcgacg aatcctacga tccgtccacg
     5401 ctggccggga ctcattctgc tcaagtacgt catggctgca tcctcatccc gtccccgaac
     5461 ccgtcacttc ggcggcgcgg cgccgccgcc cgaaccgagc gcccgccggc gccgttcgcg
     5521 gcattcctcg cagtacccgt ggatctcgat tttgaaacta tcggcgcgga agtcgttctg
     5581 ctcggccacc ccggccagca ccgccacgat ttcctccacc ggaaactcgg tcagcctgcg
     5641 gcactcctgg cagtacaggt ggccgtgcgg cacgtgcgtc acccggtcgt accgcgtgac
     5701 gccgtcgccg acattcactt cctccgccag ccccacccgg cagatcagtt tcaggttccg
     5761 ccagacggtg ctgtacccga tcgagcggtc ctggcgcctc actttctcga acagctcgtc
     5821 caccgtcacg tggtccccga gttcaaagaa cgcccggaaa accagctccc gctggggcgt
     5881 cattttgaaa cctttggtgc gaaggaactc tcgcatggcg caaccttgtg ccgatcattc
     5941 cactcaactg aaaacgattt tcaatttcat cagcatatat tactctcttt cggcctgatg
     6001 tcaaggaaaa tcgagcggct aagtggcatt tttcacatga atatatccta ttctactgca
     6061 aaaagatagc cgccggggtg ggccggcggc tgcttctttt tcggtagtgc aactttgctc
     6121 tacttaagga gaaccatttt cattgtttgg gcctcgtcac cccgttcgag gcggtagaaa
     6181 tagactccgg aggccgcccg atgccctctt ccgtccgacc cgtcccaatc gaccgcgtag
     6241 gatcccggaa tctggacttc atcgaccagc gttgccaccc gctggccgag ggcgttgtag
     6301 accgccagtt tcgtgcgcgg cgtggaccgg aaatccgctc cggtgctccg cagcgtgtac
     6361 cggatcgtgg tgctcgggtt gaatggattc gggtaattct gctcgagggt aaacgaggac
     6421 ggcatgaccg ggaacgggga accgtcgtgg atggacgtgg cctggccgcc gaagtaggcg
     6481 aggatgcggc tgatgagtgt gtctttcggc atggtcgtac cgcccgcgtt gtcgttgagc
     6541 agctcggggg cgaaggtgag caacacggtt ttgtaggtcg ggcccgcgta ggcgatgccg
     6601 cgtatctgtc cgctcggccc gccggtgaat atgctctggc cgccgctgag cacattcata
     6661 taagcgttgg ccgccgccgc attcggcgcg ccggtgatcg gccggtactc cgtgccgttg
     6721 ccgatggctg tcccggggac gccgagatac tgagggaagt agagattgcc ggcatggctc
     6781 gccttgaggt agtctgcgac gaaggcgttg tcgagggcgg ccagttttac cagcgcgtag
     6841 gtccccaggc acagatgatg gtcgggggcg ttcaggtacc ctttcatcgc ggcgacatta
     6901 gccgcgctga aagcgccggt cgaggtatcg atgcccgtgt gccagaagac gatccgatag
     6961 ccgctcagag ccagcgagtc gggcgagccc tgggactgga cattccacac ccggctggga
     7021 atcccccggc cccgcagcgc atccacatac tcctgctggt actcagcccc gttgtcgtcg
     7081 tcgaccacca gcacctgggt ggcgcccacc tgtttggaga actggaactc cttgacaaac
     7141 tcgctgccga atgtcccgcc ggggagcgag tcggtcacga tcgtcaccgt gaacgtgtca
     7201 atcgccgccg tcaggctgtc ctttacccgg aagatgatgg gatcggtgtt gtactggatg
     7261 tcgaccagca ggttcgtgcc gaaggagacg gccggggtca cccactcgat ccggggcgac
     7321 ccgctggcga gcgccgcccg cacattgtag gagtaccgca tgaaattgcg gatgtagcag
     7381 tagaagtgga tctcttcgcc ggcttcgacg acgccgtcgc cgtccccgcg cggcgcgtca
     7441 tcgttgaaga cgaacccgct cgaggcttcg acgatccagg gccgcgagta ctcgatgtcg
     7501 agttcggcgt acatgaccga atcggcgttg gagacatccc acacgccgat acgcgacacg
     7561 aggccgtaga gcggcgagag gtcgtcgttg agattcgtgg ccggcgtgct gtagtcgtgg
     7621 aagttgcggt tgccggtggc gccggggaac ggatcccccg catcgccgga gctcccggcg
     7681 tactcaagct ggaactggcc gtccgcctgc tccagcgcga tgcggtaggg cgggtaccgg
     7741 ttgctgcccg taccggcggc cgtgccggcg tcgtagtgcc agatgcacag gccggatccc
     7801 gggatgttcg tgtcaaaccc cgtctggcgg cggttctcga tcagccagta ttccgaactc
     7861 ccggtggtcg cgtttctcag ccggtagacc accgggttgt attccacggc cgggatagcc
     7921 gccagcttga tgttctgcgt cacgtcgatg a
//
LOCUS       DAOPOE010000121         7859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10344_length_7859_cov_6.134803, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000121 DAOPOE010000000
VERSION     DAOPOE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_7859_cov_6.134803"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PKM94_12340"
     CDS             complement(<1..1703)
                     /locus_tag="PKM94_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD67464.1"
                     /translation="MKPLRVTSRILKRFLESPTEILIVLSVFVGLVTALGALAFRALI
                     DFANHLFFGLTDQVLTKSLGAGSFKWWLPIVPALGGLIVGAIVLRFAAEARGHGVPEV
                     MNAVARLGGIIRPRVAAVKTIASAICLGSGGSAGREGPIVQIGSALGSTIGQLFRMSG
                     DRIKILVGCGAAAGISSVFNAPIAGVIFTLEVVLGDFALATFSPVIISSVVASVVTRS
                     VLGANPAFAVPTFALVSGWEMPLYALLGVLLGGVGFVFTRMMVACEEFFEKMAIANWL
                     KPALGGLLLGGIAVFFPQVLADGYGTIRLTLQGEMALWLMAALVFLKILATSLTLGSG
                     NSGGIFAPSLFIGAGAGGAFGAVAERLFPAATAPPGAYALVGMAGMVAAATHAPITAL
                     LIIFEMTSDFRVILPLMVVVVFAALVARRLYPHSIFTAKLIRSGIDIRGGRDVNVLRS
                     HRVAEVMSREFETVDISASLHDILVKIEESRESYFLVMDRREVLRGVISFQDIRAVLS
                     QRTLDRLVIARDIVHEEVVTLGTDDTLERAFQLFSARGLRLIPVVDGGPGERVVGVLR
                     R"
     gene            1896..3050
                     /locus_tag="PKM94_12345"
     CDS             1896..3050
                     /locus_tag="PKM94_12345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD67465.1"
                     /translation="MRSHADAVIVGGGIIGLSVAFHLAREKYGRIIVLEKEQLLGTGA
                     TSKAAGGIRAQFSTKVNIEMSMLSEKLFRQFKEDTGSDALFDQVGYMFLLQTEAEVRE
                     FTKSYELQRSLGLPAVLLKTDEIPQYAPHIKLDGVRMATFCKDDGLGDPHEFLSGYEH
                     AARRLGVEIAFESPVTGIQVESGRIRSVQTAAGRIDTPLVVNCAGPQSREVAAMAGAE
                     LPVDPIRRQIVTTGELSWVEPYFPMVVDVSSGLYFHKESKGMLFGWAKPGVKPSYDIS
                     VDPEYTDEILERTMIRIKDELLDEIAVANQWGGLYETTPDHHAVIGREPRVDGLFYCA
                     GFSGHGFMHAPAAGVVTAEILTGKRPSIDIAPLAIERFAAGVAVEETNVI"
     gene            3122..3859
                     /locus_tag="PKM94_12350"
     CDS             3122..3859
                     /locus_tag="PKM94_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67466.1"
                     /translation="MRIPIICAALTLLIAAGAAGSDQTAGSTCPSSRAAEAGYNPFEE
                     FHAVMAPAWHQAWPDSNFADLFAAGPKFQEAFGKVAEMKPALKSAAREKVFAERRAAL
                     GGLVQKYAAAAAAEDKDKVYALMPELHDAFEQAASALLPVHYPEFEGMVVTLNLILET
                     HLPKGNTDGVNGSTETLVEKLKALTPETLPEDLREHEAAIGAEFGAMSELAARMQKCC
                     ENRDMETYREHIKTLDGKVKEFIAKYI"
     gene            3985..4758
                     /locus_tag="PKM94_12355"
     CDS             3985..4758
                     /locus_tag="PKM94_12355"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD67467.1"
                     /translation="MRVLLSPVWAVTAAVLLASAAAGTDLWKAAEVTYTTSQGLEMHG
                     WLHLPAAASQPGRPLIALLPMMGTAHGAYDLFLDSLAARLRRDTTAATPTVLALDLRG
                     HGASTRLHGKEISYKEMEPGDWAPAPGEVAEFIRRAARDHPEAIDSLNITLVGASIGA
                     NAAAIAAPMVPGVRGIALLSPGSDYRGLAPAEALKAFEGRVLICAAREDRYAAESAGR
                     LAKVREGPVLRIFEGGEHGTNLIDRTPEAMSGLLDWLEE"
     gene            complement(4793..5578)
                     /locus_tag="PKM94_12360"
     CDS             complement(4793..5578)
                     /locus_tag="PKM94_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOD67468.1"
                     /translation="MNSDRPQDRPPVDKFGLIHRLAVDAAGGEEPQEVYRRGLRLSAD
                     LVGLAAAALVVWNRDHVLTLNVSHARSEDYRRRLDALERELFDRLRQTQQLASAYMSF
                     EGDPPVHAFTLPLRYAGRTYGAVIGLQEGPRTIVSEEALLETLAAVLALTWVAGSQNR
                     EEADLRSALTKERKSAVIETAVTVNHEVNNPLTAILGNVQLLLLKRDDLDDELKAKLR
                     TIEQSALKIKDVTQKLMRLTSPRSIDYSDGTKMIDLNDGGEEA"
     gene            5848..7269
                     /gene="purF"
                     /locus_tag="PKM94_12365"
     CDS             5848..7269
                     /gene="purF"
                     /locus_tag="PKM94_12365"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOD67469.1"
                     /translation="MVEDVIHDHCGVCGVFGHSRAAELTYLGLYALQHRGQESAGIVT
                     SDNGSIHYHKGMGLVSEVFANPQVLAGLTGGRAIGHTRYSTTGASSLINIQPFLITNR
                     SRHLAIAHNGNLTNSTELRTKLDARGSIFQTTSDTEIILHLAARSRKRTRLEQVCDAL
                     ARVKGAYSLLFLTEDTLIAARDPFGFRPLALGKYRKTWVVASETCAFDLIGARYVRDI
                     EPGEVLEISAGGLKSLKPFAAQRHAMCIFEYIYFSRPDSKIFGENVDKIRRRLGRLLA
                     REHPVEADIVIAIPDSANTAALGYAEESGIRFEIGLIRNHYVGRTFIDPGQQIRDLDV
                     KVKFNPVRGVLYGRRVMVVDDSIVRGTTSKKLVAMIRGAGAKAIHFRVSSPPIISPCF
                     FGIDMPTREELIGARMSVTEIEKYLKVDSLRYLSLEGMLSLKSLPDTTFCASCFSGKY
                     PMAIPKINGKDRFDIKSSGRFGI"
     gene            complement(7353..>7859)
                     /locus_tag="PKM94_12370"
     CDS             complement(7353..>7859)
                     /locus_tag="PKM94_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOD67470.1"
                     /translation="GLTAGGEDAISSALFYLIAYAFFNLGAFAIVTMLETRSGGKAEI
                     DELPGMGRKHPYLAATLALFMFALSGFPPTVGFFGKFYIFSAAVKSGFLWLTVIGVMN
                     SFLSVYYYLRLVKGVYFDAAAREFAPVTYTPALVVVLVVTAAGTLGLGFFPQQLLAFS
                     QKALFAFL"
BASE COUNT         1477 a         2502 c         2630 g         1250 t
ORIGIN      
        1 tcccgccgca gcacgcccac caccctctcc cccggtccgc catcgaccac cgggatcagc
       61 cggaggccgc gggcgctaaa gagctggaag gcccgttcga gcgtatcgtc ggtccccagg
      121 gtcacgacct cctcgtggac gatgtccctg gcgatgacca ggcgatccag cgtgcgctgg
      181 ctcagcacgg cgcggatatc ctgaaatgaa atcactccgc ggagcacctc ccgacggtcc
      241 atgacgagga aataggactc ccgcgactcc tcgatcttca ccaggatgtc gtggaggctg
      301 gccgagatat ccaccgtctc gaattcgcgg gacatgacct cggccacgcg gtgggagcgc
      361 aggacgttga cgtcgcggcc gccgcgaatg tcgatgccgc tccggatgag tttcgccgtg
      421 aaaatcgaat gcgggtagag ccggcgggcg acgagcgccg cgaacacgac gacgaccatg
      481 agcggcagga tgacccggaa gtcggaggtc atttcgaaaa tgatcaggag ggcggtgatc
      541 ggggcgtggg tggccgcggc aaccatcccg gccatcccca ccagggcgta cgcgcccgga
      601 ggggcggtcg cggccggaaa gaggcgctcg gcgaccgcgc cgaacgctcc cccggcgccg
      661 gcgccgatga acagggacgg ggcgaagatc cccccggagt tgcccgaccc gagagtgagc
      721 gaggtggcca ggattttgag aaagaccagc gccgccatca gccagagcgc catctcaccc
      781 tgcagcgtca gcctgatcgt cccgtatccg tcggccagca cctgcgggaa aaagacggcg
      841 attccgccaa ggagcagtcc ccccaatgcg ggtttgagcc aattggcgat tgccattttc
      901 tcgaagaact cctcacaggc gaccatcatc cgcgtgaaga cgaaaccaac cccgccgagg
      961 agcacgccga gcagggcgta gaggggcatc tcccagcccg agacgagggc gaaggtgggc
     1021 acggcgaaag cgggattcgc gcccaggacc gaacgggtca ccaccgaggc gacgacggag
     1081 gagatgatca ccggggagaa ggtggcgagc gcgaaatccc cgaggacgac ctcgagggtg
     1141 aaaatcacgc cggcaatggg ggcgttgaaa accgaggaaa tgccggcggc cgccccgcac
     1201 cccaccagga ttttgatgcg gtcgcccgac atgcggaaga gctgaccgat cgtcgacccc
     1261 aaggccgaac cgatctggac gatcggtccc tcccgtccgg ccgacccccc ggacccgagg
     1321 cagatggccg aggcgatcgt tttgacggct gcgacgcggg gccgaatgat acctccgagc
     1381 cgggcgacgg cgttcatcac ttcgggcaca ccgtggcccc gggcctcggc cgcgaaccgg
     1441 agcacgatcg cccccacgat caggccgccg agggccggga caatcggcaa ccaccacttg
     1501 aacgaccccg ccccgaggct tttggtcagc acctggtcgg tgagaccgaa gaacaggtgg
     1561 ttggcgaagt cgatcagggc ccgaaaggcc agcgccccca aagccgtaac cagcccgaca
     1621 aataccgaga gcacaatcag gatttcggtc ggcgactcca ggaaccgctt gaggatccgg
     1681 ctcgtgacgc ggagcggttt catacgggcg caataacggg ccggcctatg cgggggcaca
     1741 agtttttagc gcccgacccg cgcgaaaaaa gccttctgcc cacggggact tccccgccga
     1801 ccaacccgga aagcgcttga caactccccc caattcaata ccttcaacgt gaaaaagttc
     1861 actatgtttg cagacattgc agtgaggacg acatcatgcg cagtcatgcc gacgcggtca
     1921 tcgtaggggg gggcatcatc ggcctctcgg ttgcgttcca cctcgcccgc gagaagtacg
     1981 gccggatcat cgtgctggaa aaggagcagt tgctgggcac cggggccacg tccaaggcgg
     2041 cgggcggcat ccgcgcgcag ttttccacca aagtcaatat cgagatgtcc atgctctcgg
     2101 agaagctgtt ccgccagttc aaggaggata ccggctcgga cgccctgttc gatcaggttg
     2161 ggtacatgtt cctgctgcag acggaggcgg aggtccggga gtttacgaaa agctatgagt
     2221 tgcagcgctc gctcggcctt cccgccgtgc tgctgaaaac ggatgagatc ccgcagtatg
     2281 ccccgcacat caagctcgac ggggtgcgga tggcgacctt ctgcaaggat gacggtctgg
     2341 gggatccgca cgaatttctc agcggctacg agcatgccgc gcgccggctc ggagtggaaa
     2401 tcgcctttga gtcgccggtg acgggtattc aggtcgagtc gggccggatc cggtcggtgc
     2461 agacggcggc aggccgcatc gacaccccgc tggtggtcaa ctgcgccgga ccgcagtccc
     2521 gcgaggtggc ggcgatggcc ggggccgagc tgccggtcga tccaattcgc cgccagattg
     2581 tcacgaccgg cgaactcagc tgggtggaac cgtatttccc gatggtggtc gacgtctcgt
     2641 ccggcctcta tttccataag gagtcgaaag ggatgctctt cgggtgggcc aaaccgggcg
     2701 tcaagccctc atacgacatc tcggtcgatc ccgagtacac cgacgagatc ctcgagcgga
     2761 cgatgatccg catcaaggac gagctgttgg acgagatcgc ggtggccaac cagtggggcg
     2821 gcctgtacga gacgaccccc gatcaccacg cggtcatcgg ccgggagccc cgggtcgacg
     2881 gcctgttcta ctgcgccggg ttctccggcc acgggttcat gcacgccccg gcggcgggag
     2941 tggtgacggc ggagatcctc accggcaaga ggccgtcgat cgatatcgcc ccgctggcca
     3001 tcgagcggtt cgccgccggc gtggcggtgg aagagaccaa cgtcatctga acttgcgttc
     3061 atgaggcaat agaaccggag atttgccgag cggccgtgcg gccgtcggca agggaggaga
     3121 catgcggata ccgatcattt gtgccgcgct gacgctgttg atcgcggccg gcgcggccgg
     3181 tagcgaccag accgccggct ccacctgccc gtcgagtcgg gcggcggagg ccgggtacaa
     3241 tccgtttgag gagttccacg cggtcatggc gccggcctgg caccaggcct ggccggacag
     3301 caatttcgcg gacctgttcg ccgccggtcc gaaattccag gaggcgttcg gcaaggtcgc
     3361 ggagatgaag ccggcgctca agagcgccgc acgcgagaaa gtgtttgcgg agcggcgcgc
     3421 cgcgctcggc gggctggtgc agaaatacgc cgcggccgcc gccgccgaag acaaggacaa
     3481 ggtctacgcc ctcatgccgg aactgcacga cgcattcgag caggccgcct cggccctgct
     3541 gccggtccac tatccggagt tcgaaggcat ggtggtgaca ctcaatctca tcctcgaaac
     3601 gcacctgccc aaggggaaca ccgacggcgt aaacggctcg accgaaacac tggtggagaa
     3661 gctcaaagcc ctcaccccgg agacgctgcc ggaggatctg agagaacacg aagcggcgat
     3721 cggggccgaa ttcggcgcga tgagcgaact ggccgcccgg atgcagaagt gctgcgaaaa
     3781 ccgggacatg gagacttacc gggagcacat caagacgctc gacggcaaag tgaaggagtt
     3841 catcgcaaaa tacatctgac ggcgcagacc gacggatgtc tgagcgcggc ggtcgcgtgt
     3901 cgagcagccg ccgattctcc tggcgggcgg tcccgccccg gatggcgccg gccgaggagg
     3961 tggcgcatgg aatcaggagg cgacatgagg gttctccttt ccccggtctg ggcggtgacg
     4021 gcggccgtac tgcttgcatc ggcggcggcc ggcaccgact tgtggaaagc cgccgaggtc
     4081 acgtacacga cctcgcaggg gctggaaatg cacggctggc tccacctgcc ggcggcggcg
     4141 tcccaacccg gccggccgct gatcgcgctg ctgccgatga tgggcactgc tcacggcgca
     4201 tacgacctgt tcctggattc gctggcggcg cggctacggc gagacacgac ggccgcgacg
     4261 ccgaccgtgc tcgcgctcga cctgcgcggc cacggggcct cgaccagact gcacggcaaa
     4321 gaaatttcgt acaaagagat ggagccgggg gactgggcgc ccgccccagg cgaggtggcc
     4381 gagtttatcc gccgcgcggc gcgggaccac ccggaggcca tcgactccct gaacatcacg
     4441 ctggtcggcg cgtcgatcgg cgccaatgcc gccgcgatcg ccgcgccgat ggtcccaggg
     4501 gtccggggaa tcgcgctgct gtcgccgggc agcgactacc gggggctcgc accggcggag
     4561 gcactgaagg catttgaggg ccgcgtgctc atctgcgccg cccgcgagga tcgctacgcc
     4621 gccgagtccg ccgggcgact ggcgaaagtg cgggagggac cggtgctgcg catcttcgaa
     4681 ggcggcgaac acggaacaaa cctgatcgac cgcactcccg aggcgatgag cgggctgctc
     4741 gactggctgg aggagtgacg cactttcgga agtcgacgcg ggcggccggg gtctaggcct
     4801 cctctccccc gtcgttgagg tcgatcatct tggtgccgtc ggaataatcg atcgaccggg
     4861 gagaggtcag gcgcatcagt ttctgtgtga cgtctttgat cttgagggcg gactgctcga
     4921 tggtccgcag tttcgccttg agttcatcgt cgagatcatc gcgcttgagc agcagcagtt
     4981 ggacgttgcc gaggatggcg gtcagcgggt tgttgacctc gtggttgacc gtcaccgccg
     5041 tttcgatgac ggcgctcttg cgttccttgg tgagtgcaga gcgcagatcg gcctcttccc
     5101 ggttctgcga gccggccacc caggtcagcg cgagaacggc cgcgagggtt tccagcagag
     5161 cctcctcgga cacgattgtc cgcggcccct cctgcagtcc tatgaccgcg ccgtaggttc
     5221 tcccggcgta gcggaggggg agggtgaagg cgtgcacggg cgggtcgccc tcgaacgaca
     5281 tgtacgccga ggccagttgc tgggtctggc ggaggcggtc gaacaactcc cgctcgaggg
     5341 catcgagccg gcggcgatag tcttcgctgc gggcgtggct gacattgagg gtgaggacgt
     5401 ggtcacggtt ccacaccacc agggcggcgg cggccagccc gaccaggtcg gcgctcaggc
     5461 gcagcccccg ccggtagacc tcctggggct cctcgccccc ggcggcgtcg accgccaggc
     5521 ggtgaatgag gccgaattta tcgacgggcg ggcggtcctg tggccggtca ctgttcatcg
     5581 tgtctcattt gagcaaacgc ggtgccccgc ggcgccgggc gcccgcgcgc tggagtaact
     5641 ctgaggtacc cttgaaaata aggagttttg ggcctatgtg ccatgagttt tcgcagccgg
     5701 ccggccgtgc gggagaaccg atccgccggc ggatcggtgc atttgtgttc acagacatat
     5761 gcaaaatgcg gcgctcgaat cgttgacatt tatccgagcc gggcgtatac tgggtgtttt
     5821 ccaaggcgcc catgaggagc taacggcatg gtagaggacg tgatacatga ccactgcggc
     5881 gtctgcgggg tattcggtca ctcccgcgcc gccgaactca cctacctggg gctctacgcg
     5941 ctccagcacc ggggccagga atcggccggc attgtcacct ccgacaacgg ctcgatccac
     6001 taccacaagg ggatgggact ggtgtccgag gtcttcgcca acccccaggt cctggccggc
     6061 ctgacgggcg gccgggcgat cggccacacc cgttactcga ccaccggggc ctcctcgctg
     6121 atcaacattc agccattcct gatcaccaac cgcagccgcc atctggcgat cgcccacaac
     6181 ggcaacctga ccaactccac cgagctgcgc acaaaactcg acgcccgcgg ctcgattttt
     6241 cagacgacct cggacactga aatcatcctc cacctggcgg cgcggtcccg caagaggacg
     6301 cgcctggagc aggtttgcga cgccctggcg cgggtcaagg gggcctattc gctgctgttt
     6361 ctcaccgaag acacgctcat cgccgcccgc gacccctttg gtttccggcc gctggcgctc
     6421 ggcaagtacc gcaagacctg ggtggtggcg tcggagacct gcgcgttcga tctgatcggc
     6481 gcccggtatg tgcgggacat cgagcccggc gaggtgctcg agatctcggc cggcgggctg
     6541 aagtcgctca agccgttcgc ggcgcagcgg cacgccatgt gcattttcga gtacatttac
     6601 ttctcgcgcc cggactcgaa aatcttcggc gagaacgtcg acaagatccg ccgccggctg
     6661 gggcggctgc tggcccgcga acatccggtc gaggcggaca tcgtcatcgc catccccgat
     6721 tcggccaaca ccgcggctct cggctacgcc gaggagtcgg gcatccgctt cgagatcggc
     6781 ctcatccgca accactacgt ggggcggact ttcatcgatc ccggccagca gatccgcgac
     6841 ctcgacgtca aggtgaagtt caacccggtg cgcggcgttc tgtatgggcg gcgggtgatg
     6901 gtggttgacg actccatcgt gcgggggacg acctcgaaga agctggtcgc catgatccgc
     6961 ggcgccgggg ccaaggcgat tcacttccgg gtgtcgtctc cgccgatcat ctccccctgc
     7021 tttttcggga tcgacatgcc gacgcgggaa gagttgatcg gggcgcgcat gtcggtgacc
     7081 gaaatcgaga agtacctcaa ggtagattcg ctgcgctacc tgtcgctgga ggggatgctg
     7141 tcgctgaaat cgctccctga cacgaccttt tgcgccagct gcttctcggg caagtacccg
     7201 atggcgatcc caaaaatcaa cgggaaggac cgcttcgaca tcaagagttc cgggcggttc
     7261 ggcatttagc cgaaagctcg atcggtcaaa tggaacggca ggagcttccg ctcccgcctt
     7321 cggcattttt gtcgcggccg tcccggccaa tgtcacagga acgcgaagag cgctttctgc
     7381 gaaaaagcca gcaactgctg cgggaagaag cccagtccga gcgtgcccgc ggccgtgacg
     7441 accaggacca caaccagcgc cggcgtgtag gtgaccggcg cgaattcccg cgcggcggcg
     7501 tcgaagtaca ctcccttcac cagacgcagg tagtagtaga ccgacaagaa ggaattcatc
     7561 acgccgatca cggtcagcca gaggaatccg gatttgacgg cggccgagaa gatgtagaat
     7621 ttcccgaaga atccgacagt gggggggaaa ccggagagcg cgaacatgaa cagggcgaga
     7681 gtcgccgcca gatacggatg tttccgaccc atccccggga gctcgtcgat ctcggctttg
     7741 ccgccggagc gtgtctccag catggtgacg atggcgaacg cgccgaggtt gaaaaaggcg
     7801 taggcgatca ggtagaacag ggccgaggag atggcgtcct ctccccccgc ggtcaggcc
//
LOCUS       DAOPOE010000122         7815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10415_length_7815_cov_4.931314, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000122 DAOPOE010000000
VERSION     DAOPOE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_7815_cov_4.931314"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKM94_12375"
     CDS             <1..255
                     /locus_tag="PKM94_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOD67471.1"
                     /translation="RIDLSNGGVATSGDARRFCLVEGVRLGHILNPRTGWPVADAPRS
                     VTVLGDYCVEAGFLATLAMLHGRAAEDFLAGEKVPYHCIR"
     gene            complement(261..2411)
                     /locus_tag="PKM94_12380"
     CDS             complement(261..2411)
                     /locus_tag="PKM94_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD67472.1"
                     /translation="MTRQSAAAVLAVVLVLAAPTGSPWADLADGPVHTILWNRMDLVE
                     VVDSLAVGFSSEGVTVCRWSDSLNQFVVESVHEIDYYPAAVRMAEGRLMVHTADQRLI
                     AYDAAMLPELVTAWEFAPGAAFADWALVDTQLYLSRWFEGLWRYAIGPAGTAALLDTS
                     MVGIMVTQMEAEGPWLYVLDEYNGIMRYDPAGAGSAAFLDYLYVPQEVLSFAQADSTS
                     AMIGRSGGVVIGRYPGPQSLVAPAVPDGDRPQRVYLSGDAMVTVAPRSIEVRDRGTGE
                     VRAAMAIEGVVTDGALFTRGGTRYLVLPREAGGLFLVNIDDLGQSGPGLYRSGPVQAL
                     AFGGGKLFTSGGHNPIEAYTFDSTLAPHLDFALLPHLNSMQALTVTGDTLLAYMAGIN
                     TIVVIRRPFFADSFLVDRAITLDDTMAGRIVFLPRLRHDSLRAIAVTGLRTMQVFTIN
                     DSGRVSSQEPWEQQFGITASCLTDTMVFLATGKDQIWSYRIGPDLRTQLAGITDLPSQ
                     ASVMVWDSGRLLVFVRDQLLVSRLLPSGALSVESWIDMPFTASDAVISGRGLFAVGTT
                     GIGVFRLDREVPELAEYGGDPGTVIAVSGEMVAASDGGSVKIYRLGVATGVDPEPELP
                     RRFALDQNYPNPFNGETVIRYSLAEGARVRLAVYNVLGQRVKTLVDEYRAAGIYEALW
                     DGTDRAGAPVASGVYLYRLEAGKAAGARKMVFVK"
     gene            complement(2408..>6069)
                     /locus_tag="PKM94_12385"
     CDS             complement(2408..>6069)
                     /locus_tag="PKM94_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOD67473.1"
                     /translation="AGFAARGDSGPVPGAFIVKLAPGVNPAAVRQALGGRERLTPVVP
                     GGLKAGLAGREDLERVYFLATERPAAAAADITDMLGRGNVEWVEPEYFLELFELPADS
                     LFPRQWHLLNTGQEYFGINRIDGAYNDERVIKRGTAGRDVRLVGQYQSPPADSAEKVV
                     VAIIDTGVDTDHPELAGRVWRNLDEIPGNGGDDDHNGYIDDTAGYDISGDTSNLFDPA
                     GDADPRDEDGHGTHCAGIVGANGDGAGVVGIAPWIEIMPVKIFPNGTTRIGGLGILYA
                     VNNGARVINASWGSPFQSQFLREVINFARENGVLVCMASGNSGDNSRFYPAGFDLDST
                     LVVGAGNSDGELTYFSTFGSHVDIIAPGLDILSLRAAGTDMYAAYPAYEPEVRIVDSL
                     YYLSDGTSMAAPMVCGAAGMLLSFRPDLSLGELVAILKHGATDLVDPLNQGDDLPGPD
                     SLCGWGYLNIDASLGLMESGGMYLTAPEHRSRHTGDIIIKGGTIGGYDDGWTIEYATG
                     LSPEAWHFLAAGNGLPPDSVLAVFRNPGAAGLLSFRLTDSYGRSGTVTVTYAPTNVLA
                     MSSPADGMTFDYAIPVRGSAYGPDFDSLVVKSRGPRGNVVRLFAGTGEVFDSLFYTWP
                     ASGADTGAFTLYLYGYFATGVEGDSAGITVTSAFADGWPRRIPGWGGMTAVSADLNRD
                     GSKELIAATSGGLAVYRAADGELLSGFPALAGRDVRCVPAVYDVDGDGHDEIIVTDST
                     GLHVIRDDGTAAPGWPQACFTGMTPYEYGFPAPVIVELRRGSPPGAVPDSGILFINRL
                     GQLIAFRFNGDRYFYSRKMWGQFDARISPSYLTGGDTSPFATAADVDGDGRREVCASF
                     SSANPHTGMGLFDGANGRPAFGRDVPTIQTIASVSGSVLADLDGDGLPEAITTGLGDD
                     DLEHVWIKTLGTDDFASWVVPIDDSWIGWIASYPVVADLDRDGTPEILMTYFEFDQAA
                     LFIFRADGTPYATGPRGIPGEAFYDYATFATPAVANIMGDEYPEIIFRAGHLLPGTGP
                     ELLYILDYAAVPVAGWPKRTPAPSATVASGRQVPLVDDLDNDGKVELALLSDEQSLLV
                     WDFEGSYDEGRNTFRWMYDNGNSGIYPGSQAAPQSSDGPARVRKAQRSAAGEARAVGR
                     ATAPVRFNLPAPARVAVEIYDTAGKRVATLPERMMEAGDQEMDFSEAALAPGTYFYRL
                     IVDGTAFSRRMAVIE"
     assembly_gap    6070..6079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6563..>7815
                     /gene="asnB"
                     /locus_tag="PKM94_12390"
     CDS             6563..>7815
                     /gene="asnB"
                     /locus_tag="PKM94_12390"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD67474.1"
                     /translation="MCGIAGYFQLESPRPPDRALIGRMVNTLHHRGPDEFGAYLDDRC
                     VLGQARLSIIDLAGGSQPMCNEDGSLWITFNGEIFNYLELREELVGAGHVFRTRCDTE
                     VIIHAYEQWGRDCLTRFNGQWAFVLYNRRDRSLFFARDRLGVRPLFYAVHGGAFYFAS
                     EIKAIFCAPEIPRRLDLKGLDEIFTWWTTAPPRTAFEGIHELEAGACGVVRNGRVRTE
                     RYWSMEFPETFDRRRPVESWAEELHALLVDAVRLRLRADVPVGAYLSGGLDSSATTAL
                     IRHFTDTRVESFSIAFQDKAYDESPFQRQMAEHLGTNHHVVTCTYRDIAENFPAVIRH
                     TERPIVRTAPTPLFLLSRLVRENNFKVVLTGEGSDEILGGYDIFKETMIRRFWARRPD
                     SAWRPALLKRLYPTLPLSGARAKFYL"
BASE COUNT         1336 a         2794 c         2369 g         1306 t
ORIGIN      
        1 cgaatagacc tcagcaacgg cggcgtcgcc accagcggcg acgcgcggcg gttctgcctg
       61 gtggaaggcg tgcggctggg tcacattctc aacccccgca ccgggtggcc ggtggccgat
      121 gctccccgaa gtgtcacggt tctgggagac tactgcgtcg aggccgggtt cctggccacc
      181 ctggccatgc tgcacggccg ggcggccgag gacttcctcg ccggagaaaa agtcccttac
      241 cactgcatcc ggtaggcccc ctacttcacg aaaaccatct tcctggcccc ggcggccttc
      301 ccggcctcga gccggtagag gtagaccccg ctcgccaccg gcgcccccgc gcgatccgtg
      361 ccgtcccaca gcgcttcata gatccccgcc gccctgtact catccacgag cgttttcacg
      421 cgctgcccga gcacgttgta gaccgcgagc cgcacgcgcg ctccctccgc cagagaatag
      481 cggatcaccg tctcaccgtt gaacggattc gggtagttct ggtcgagcgc gaaccgccgc
      541 ggcagttccg gttcgggatc cacgcccgtg gccacgccca ggcggtaaat cttcacagaa
      601 ccgccgtccg aggccgcgac catctctccc gacactgcga tgacggtgcc cgggtccccg
      661 ccgtactccg ccaattccgg cacttcccgg tcgagacgga agaccccgat ccccgtggtt
      721 ccgaccgcaa acagcccccg ccccgagatg accgcatcgc tcgccgtgaa cggcatatcg
      781 atccagctct cgacagagag cgctccgctc ggcaggagac gggacacgag aagctggtcg
      841 cggacgaaca cgaggaggcg gccgctgtcc cagaccatca cggaggcctg cgagggaagg
      901 tcggtgatgc cggccaactg cgtgcggaga tccggcccga ttcgatacga ccagatctgg
      961 tctttccccg tggccaggaa gaccatcgtg tccgtgaggc aggatgcggt gatcccgaac
     1021 tgctgctccc acggctcctg cgaactgacc cggccggagt cgttgatcgt aaagacctgc
     1081 atagtgcgca gccccgtgac ggcgatcgcc cgaagggagt cgtgccgcag gcggggcaga
     1141 aagacgatcc gccccgccat cgtgtcgtcc agggtgatcg cccggtcgac gaggaaggaa
     1201 tcggcgaaga agggccggcg gatcaccaca atggtgttga tgccggccat gtaggccagg
     1261 agagtgtcgc ccgtcaccgt cagcgcctgc atgctgttca agtggggcag gagggcgaag
     1321 tccaggtggg gcgcgagcgt ggagtcaaac gtgtaggcct cgatcgggtt gtggccgccc
     1381 gacgtgaaca gtttgccgcc gccgaacgcc agcgcctgca ccggcccgga gcgatacagc
     1441 cccggcccgc tttgcccgag gtcatcgata ttcaccagaa acagcccgcc ggcttcgcgg
     1501 ggcagcacca ggtagcgggt tccaccccgg gtgaacagcg cgccgtcggt caccacgccc
     1561 tcaatcgcca tcgcggcccg cacctcgccg gtcccccggt cgcgcacctc aatggagcgc
     1621 ggcgccacgg tcaccatcgc atccccgctc aggtacaccc gctgcggccg gtcgccgtcg
     1681 ggaaccgcgg gcgcgacgag cgactgcggc ccgggatacc gcccgatgac gaccccgccg
     1741 gaccgtccga tcatggccga agtcgagtcc gcctgcgcga acgaaagaac ttcctggggc
     1801 acgtagaggt aatcgagaaa ggccgccgac cccgccccgg ccgggtcgta gcgcatgatc
     1861 ccgttgtatt cgtcgaggac atacagccag ggtccctccg cctccatctg cgtcaccata
     1921 atccccacca tgctcgtgtc aaggagcgcg gccgttccgg ccggtccgat cgcatagcgc
     1981 cacaggccct cgaaccaccg cgacaggtag agctgggtgt cgacgagcgc ccagtcggcg
     2041 aacgccgccc cgggagcgaa ctcccaggcg gtcaccagtt cgggcagcat cgccgcgtcg
     2101 tacgcaatca gtcgctggtc ggcggtgtgc accatgaggc gtccctccgc catccgcacg
     2161 gccgctggat agtagtcaat ctcgtgcacg ctctctacga caaactggtt gaggctgtcc
     2221 gaccaccggc agaccgtcac cccttcggac gagaacccca cggcgagcga atcgacgacc
     2281 tctacgaggt ccatccggtt ccacagaatg gtgtgcaccg gcccgtcggc caggtccgcc
     2341 caaggcgacc ctgtcggcgc cgccagcacc agaacgaccg cgagaacagc cgccgccgat
     2401 tgccgtgtca ttcgatcacc gccattcgcc gcgaaaacgc cgtgccgtcc acgatcagcc
     2461 ggtagaaata cgttcccggc gcgagggccg cctcggagaa atccatctcc tggtcccccg
     2521 cttccatcat tcgctccggc agcgttgcga cccgcttgcc ggcggtgtcg taaatctcga
     2581 ccgccacacg cgccggggcg gggagattga agcgcaccgg cgccgtcgcc cgaccgaccg
     2641 ccctcgcctc gccggccgcc gaccgctgcg ccttgcgcac ccgagccggg ccgtccgaac
     2701 tctgcggcgc agcctgcgac cccgggtaga tgccgctgtt cccgttgtcg tacatccagc
     2761 gaaacgtgtt ccgcccctcg tcgtaactcc cctcgaaatc ccacaccagc aggctctgct
     2821 cgtcgctcag caaggcgagt tcgactttgc cgtcgttgtc gaggtcatcg accagcggga
     2881 cctgccgccc cgaggccacg gtcgccgacg gtgcgggcgt ccgtttcggc cagccggcca
     2941 ccggcacggc cgcatagtcc aggatgtaga gcagctccgg accggtcccc ggcagcaggt
     3001 gcccggcccg gaagatgatc tccgggtact cgtcccccat gatattcgcc acggccgggg
     3061 tcgcgaaggt tgcgtagtcg taaaacgcct ccccgggaat tccgcgcggt cccgtcgcgt
     3121 acggcgtgcc gtccgcccgg aaaataaaga gcgcggcctg atcgaattcg aaataggtca
     3181 tgagaatttc cggcgtcccg tcgcgatcca gatcggcgac caccgggtag gaggcgatcc
     3241 agccgatcca cgagtcgtcg atcggcacca cccagctcgc gaaatcatcg gttccgagag
     3301 tcttgatcca cacgtgctcg agatcatcgt cgcccaggcc ggtcgtgatc gcctccggca
     3361 gcccgtcgcc gtcaagatcc gccaggacgc tgccgctcac cgaggcgatt gtttgaatgg
     3421 tcgggacgtc cctcccgaaa gccgggcggc cgttggcccc gtcaaacagc cccatgccgg
     3481 tgtgggggtt ggccgagctg aaggacgcgc acacttcccg ccgcccgtcg ccgtcgacat
     3541 ccgcggccgt tgcgaagggc gaggtgtctc ccccggtcag gtacgagggg gatatccgcg
     3601 catcgaattg accccacatc ttccgcgagt agaagtacct gtcgccattg aagcggaagg
     3661 caatgagctg tcccagccgg ttgatgaaca ggatgcccga atcgggcact gctcccgggg
     3721 gcgaaccgcg gcgcagctcg acaatcaccg gcgccgggaa cccgtattcg tagggggtca
     3781 tgcccgtgaa acaggcctgc ggccagccgg gcgcggccgt gccgtcgtcg cgaatcacgt
     3841 ggagcccggt cgaatcggtg actatgatct cgtcatggcc gtcgccgtcc acatcataca
     3901 cggccggcac gcagcgcacg tcgcgccccg cgagcgccgg gaagccgctc agcagttctc
     3961 cgtcggccgc ccgatacacc gcgagtcccc cggacgtggc cgcgatcagt tctttggaac
     4021 cgtcccgatt gagatccgcg ctcaccgccg tcatcccccc ccagccgggg atccgccgcg
     4081 gccatccgtc ggcgaaagcc gaagtcaccg tgatcccggc cgaatcccct tccacaccgg
     4141 ttgcgaaata accgtagagg tacagggtga acgcccctgt atcggccccc gaggccggcc
     4201 aggtatagaa gagcgagtca aatacctccc cggtccctgc aaaaagccgc acgacattcc
     4261 cgcgcggtcc gcggctcttg accaccagcg agtcgaaatc gggtccgtaa gccgaccccc
     4321 tgacggggat cgcatagtcg aacgtcattc cgtcggccgg cgacgacatc gccagcacat
     4381 tcgtcggcgc atacgtcacc gtcactgtcc cgctgcgccc gtagctgtcc gtgaggcgga
     4441 aactgagcag tcccgccgcg ccggggttgc gaaaaaccgc gagcaccgaa tcgggcggca
     4501 ggccgttgcc ggcggcgagg aagtgccagg cctccgggct caacccggtc gcgtactcga
     4561 tcgtccaccc atcgtcgtag ccgccgatcg ttcctccctt gataatgatg tcgccggtgt
     4621 ggcggctgcg gtgttcgggc gcggtgaggt acatgccgcc gctttccatc agaccgaggg
     4681 aggcgtcgat attgaggtac ccccagccgc agagcgagtc tgggccgggc agatcatccc
     4741 cctggttgag cggatcgacc aggtcggtcg ccccgtgctt caggatggcc acgagctcgc
     4801 cgagcgacag gtcggggcgg aacgacagca gcattccggc cgccccgcac accatgggcg
     4861 ccgccatcga cgtcccgtcc gagaggtagt agaggctgtc gacaatccgc acctccggct
     4921 cgtaggccgg gtaggcggcg tacatgtcgg tccccgcagc ccgcagcgac aggatgtcca
     4981 ggcccggggc gatgatgtcg acgtgcgagc cgaaggtcga gaagtaagtc agctctccgt
     5041 cggagttccc ggcccccacg accagcgtcg aatccaggtc gaacccggcc gggtagaacc
     5101 ggctgttgtc gccgctgttg cccgaggcca tgcagacgag cacgccgttt tcccgcgcga
     5161 agttgatgac ctcgcgcagg aattgtgatt ggaagggcga gccccagctc gcgttgatca
     5221 cccgcgcccc gttgttcacg gcatagagga tgccgagccc gccgatcctc gtggtcccgt
     5281 tcgggaaaat cttcaccggc atgatctcga tccacggcgc gattcccacc actcccgcgc
     5341 cgtcgccgtt cgctcccacg atccccgcgc agtgcgtccc atgaccgtcc tcgtcgcgcg
     5401 ggtcggcgtc acccgccgga tcgaagaggt tgctggtatc ccccgaaata tcgtacccgg
     5461 cggtgtcgtc gatatatcca ttgtgatcgt cgtcgccgcc gttgccggga atttcatcga
     5521 ggttccgcca gacccgtccg gccagttccg ggtgatccgt atccacgcct gtgtcgatga
     5581 tcgcgacgac cactttctcg gccgaatccg ccggcggcga ctggtactgg ccgaccaggc
     5641 gcacgtccct ccccgccgta ccccgcttga tcacgcgctc atcgttgtat gccccatcga
     5701 tgcggttgat tccgaaatac tcttgccctg tattcagcag atgccattgg cgcgggaaca
     5761 gggaatcggc gggcagttcg aagagttcga ggaagtattc cggctccacc cattcgacat
     5821 tgccgcggcc gagcatgtcc gtgatgtcgg cggccgccgc ggcaggccgc tcagtcgcca
     5881 ggaagtatac gcgctccaaa tcctcccggc ctgcaagtcc ggccttcagt ccgcccggca
     5941 cgacaggggt cagccgctcg cggccgccca gcgcctgccg caccgccgcc ggattgaccc
     6001 ccggcgccag cttgacaatg aacgcgcccg gcaccggccc gctgtcgccg cgcgccgcga
     6061 accccgccgn nnnnnnnnng cgagaaccgc cacccatgcg gccggccaca ggatcatccg
     6121 ccggagttgt ttcatcgtca cctgggaagc acttttgact ggccgcggct gtagcatact
     6181 ctccggtttg cctcaggttg agatcccccc cgtccttttg tccctcctca gaaacgtccg
     6241 gcgtcggccg tttgttcaag gggtgcagcg tggtactgcc gacgccaccc caagatatgc
     6301 caatcccccg ccgtgtcaat ccgcaagtgc cgggccgcgg cctctgcgaa aacaactgat
     6361 tgatcttgta attgccgttc tagtagtatg ttacgatcac tatatcaact gttagtttat
     6421 atcattctcg agaacgacaa caacgacatt tcttgccccc ggccggcgaa cctcgtataa
     6481 tccactccgg cctctggaga aggtccgcgt tcatccttcc gatattagga gcaagcaaca
     6541 tccgactgag gcgctgaaga gtatgtgtgg aatagccggc tactttcaac tggagtctcc
     6601 ccgccctccc gaccgggcgc tgatcggccg gatggtcaac acgctgcatc accggggccc
     6661 ggacgaattc ggcgcctacc ttgatgatcg ctgtgtactc ggccaggccc gcctctcgat
     6721 catcgacctc gccgggggat cgcagccgat gtgcaacgag gacgggtcgc tgtggatcac
     6781 cttcaacggc gagatcttca actacctcga attgcgcgag gagctcgtcg gcgccggcca
     6841 cgttttccgc acccgctgcg acaccgaggt catcatccac gcctacgagc agtggggccg
     6901 cgactgcctc acccgtttca acggccagtg ggcgtttgtc ctgtacaacc ggcgcgaccg
     6961 ctcgctcttt tttgcgcgcg accgcctcgg cgtccgccct ctgttctacg ccgtgcacgg
     7021 cggcgccttc tacttcgcct ccgaaatcaa ggccatcttc tgcgccccgg agattccccg
     7081 ccgccttgac ctgaaaggcc tcgatgagat ctttacctgg tggacgaccg cgccgcctcg
     7141 cactgctttc gagggcatcc acgaactcga ggcgggcgcc tgcggcgtcg tccggaacgg
     7201 ccgggtccgc acggaacgct actggtcaat ggaatttccc gagaccttcg accgccgccg
     7261 cccggtcgag tcgtgggcgg aggagctcca cgcccttctg gtcgacgctg tccggctgcg
     7321 gttgcgcgcc gacgtcccgg tgggcgccta cctctccggc gggcttgatt cctcggccac
     7381 caccgcgctc atcaggcatt tcaccgacac ccgggtcgag tccttttcga ttgcctttca
     7441 ggataaggcg tatgatgagt cgccgtttca acggcagatg gccgagcacc tcggcaccaa
     7501 ccaccacgtc gtgacctgca cctaccgcga catcgcggag aatttccccg ccgtcatccg
     7561 ccacaccgaa cgccccatcg tccgcaccgc gccgacgccg ctcttcctgc tctcccggct
     7621 cgtccgggag aacaacttca aggtcgtcct gaccggcgag ggctcggatg agatcctcgg
     7681 gggatacgac attttcaaag agacgatgat ccggcggttc tgggcccgcc gtcctgattc
     7741 cgcctggcgc ccggctctac tgaaacgcct ttacccgacc ctccccctct ccggcgcccg
     7801 ggcgaaattc tacct
//
LOCUS       DAOPOE010000123         7772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10474_length_7772_cov_4.734353, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000123 DAOPOE010000000
VERSION     DAOPOE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10474_length_7772_cov_4.734353"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKM94_12395"
     CDS             <1..843
                     /locus_tag="PKM94_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD67475.1"
                     /translation="MEMRARMVVEGFIAGLHKSPYHGFSVEFAEHRQYMPGDNIRAID
                     WKVYAKSDRFYIKQYEEETNLKAYLLLDCSASMAYHSGERIRKLDYAGLLCGALSYLM
                     LRQRDAVGLVTYDRKIRRYIPPRSRSGHLHVLLNEIAAQAPSDTTDISSTLHEMADRI
                     KRRGLVIILSDLIDEADKLISGLKHFRYNNHEVIVFHILDPRERDFAFPREAVFKDME
                     TGEELTTLPYQIKKDYAKAVAAFSSEIASACRQAAIDYHPIDTAMPFDKALYAFLSKR
                     ERLY"
     gene            978..1334
                     /locus_tag="PKM94_12400"
     CDS             978..1334
                     /locus_tag="PKM94_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HOD67476.1"
                     /translation="MSTAAWSVLILAAVLIGHLVEEAATGFRRKFPLGEMPMWVFVMG
                     NVAVYGFCAATFTLVLLARPAGTLMAWVLAVAMALNGIVHIGVMIYRKEYFPGGITAG
                     LVLAAAAGLMAALARG"
     gene            complement(1473..2513)
                     /locus_tag="PKM94_12405"
     CDS             complement(1473..2513)
                     /locus_tag="PKM94_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67477.1"
                     /translation="MNPLPDPNFLPAPLWLVTVLHLLTLTLHFAAMNFLVGGLLIVVF
                     GRLADKWRHPVVAQFVKLFPTAMAATVTLGVAPLLFVQLVYPVPVYSAAIVTAWFFLG
                     IVGAVMVAYYLLYAAAFGAPGSPRRPAFLTAALLLLAYVAFAYSNLFSIAERPGLAAV
                     LYAGDPSGLRVNPHLGQWLWRWLHMLLGAVTIGGFFIGLLGRRHEDAFALGRNFFLWG
                     MVGAMAVGLVYLLSLADILKPFMHTPAIWWLSASIILSLGSLHFFFTRKFFGAGAMLF
                     VSLLGMVVVRHNVRLLQLEGHFDPAAWPIRPQWDVFLVFLVCFVLALGLVWYMLRLFF
                     ARPSPGASARDA"
     gene            complement(2510..4051)
                     /locus_tag="PKM94_12410"
     CDS             complement(2510..4051)
                     /locus_tag="PKM94_12410"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOD67478.1"
                     /translation="MNYPFWDTTLGYGVLMAVIAVVHVFVSHFAIGGGLYLVVAERAA
                     RRRGDDDHLGFLRRLSRFFVLTTVVFGALTGVGIWFIIGLLNPAATAVLIRNFVWGWA
                     AEWCFFIIEILAALLYYYGWDCLTPRRHLIIGWIYFGAAWLSLVIINGIVTFMLTPGD
                     WLATGRFWDGFFNPTYWSSLVLRTGICIMLAGLCALAVAARLPAPGARVRLVRYNAVW
                     GLVGLAVVIPSLFWYVGDIPADLMAAARQTMNIPFAALDLLYLLTAAIAALLAILGLL
                     APRAWRTEVAAVALALGLVWFGSYEWFREALRKPYVITGYMYASGVEVARAADYRADG
                     MLAHMAYRTGDEGADLFRRACRSCHSLENYNALKPFFDGADEEFITAMVQGIGAMRGN
                     MPPWTGTPEEARLLAAHLAARTDRRPFAEVCGLSGVDLGRRVYEVRCGSCHVLGGFND
                     KTQSLAGLSEEEYRTLLDMSDDLGEEMPPFTGGEPERVALIQFLQTLSAPAGTPAAAT
                     RGGAR"
     gene            complement(4160..4594)
                     /locus_tag="PKM94_12415"
     CDS             complement(4160..4594)
                     /locus_tag="PKM94_12415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD67479.1"
                     /translation="MTRHLVKLTIADYDALINLWGDAGLPYRPLGRDSRERIEIEMRR
                     PDTAFFGVFDRDRLVAAGLATFDGRKGWINRLAVHPDFRRQGLAARIVRACEEFLHER
                     GAEIIACLIEDWNEPSMELVARLGYDHAPDIEYFSKRKSADT"
     gene            complement(4679..5356)
                     /locus_tag="PKM94_12420"
     CDS             complement(4679..5356)
                     /locus_tag="PKM94_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOD67480.1"
                     /translation="MTRRYPTYRLGPDFRKRLALFRRRIVDQGFAEFAAEFAGLDEFI
                     CRAWAEDSPEKDLRRTPKEKYLLEALAFSFYDDLNREAFNRTGDTLIIVPDCLSLNNP
                     DCRKVEGEYGDTCQQCEPACQSYWVMELAGEYGARVAFSKRKLSNQIEHYAERLSNVG
                     VVGVACMIMLTSGLRTCAELDIPARGVLLSFTGCEHWNDVPFGSRFPMSWLREILEEK
                     RAARLSP"
     gene            complement(5411..5950)
                     /locus_tag="PKM94_12425"
     CDS             complement(5411..5950)
                     /locus_tag="PKM94_12425"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67481.1"
                     /translation="MRQPALTRLYAAATLVLVLGGCSGGMVTTKAVRVDGPPTAADSA
                     DSAAVLPADTDEQFRSLVSDGFMLYRQQRYPEAVAVLRRAVARHPDHWEPFYALGLIR
                     CAAEDYATAEAFLSESLDLAPPDSRIRSQIHLARARNFEAQARYGRAEQSYRAALALH
                     PESSDARDGLRRLTERPAR"
     gene            6099..6380
                     /locus_tag="PKM94_12430"
     CDS             6099..6380
                     /locus_tag="PKM94_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67482.1"
                     /translation="MANGTTPVFYKNPGLAAVLSFFWMGLGQIYNGQIAKGVFFIVLY
                     AVSVLMMLILIGFLTTPVLWIWGMYDAYSSAEKLNRELASRQGSGPAGY"
     gene            complement(6400..7173)
                     /locus_tag="PKM94_12435"
     CDS             complement(6400..7173)
                     /locus_tag="PKM94_12435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD67483.1"
                     /translation="MTPGAPYAGLAAVWDLMGQDRHSARMVDYTLALMKRHRIRAVTG
                     LDLCCGTGTALARFLDRGFIMAGLDGSPEMIAQAARKLRGRGVRLYQKTLPAFRLIPD
                     DRPRGTVRVDFITCFYDSLNHLLTERDLGAAFRSVHDHLNDRGWFIFDMNTAAALSDI
                     WSGHVFADAREELAWVWKSEYQARSRTACLTAATFVKRGDRWDRFDEIHYERAYSNTR
                     IRQLLRSAGFTVRGFYDCYRFTPADRSTLRVCVVARRRT"
     gene            7443..>7772
                     /locus_tag="PKM94_12440"
     CDS             7443..>7772
                     /locus_tag="PKM94_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD67484.1"
                     /translation="MRLAVISDVHLGDPMCQVVVREGGRCRPGPRLEQLQAALAPGLT
                     YLVLIGDILDFSLATYEEVYEAAEVFFGALAGTAEYFVYVPGNHDFDVWTTVEYQVNI
                     INRLDRGK"
BASE COUNT         1487 a         2236 c         2872 g         1177 t
ORIGIN      
        1 atggagatgc gggcccggat ggtggtggag gggtttatcg cggggctgca caagtccccc
       61 taccacgggt tctcggtgga attcgccgag caccggcagt acatgccggg ggacaacatt
      121 cgggctatcg actggaaggt gtacgccaag agcgaccggt tctatatcaa gcagtacgag
      181 gaggagacca acctcaaggc gtacctgctg ctggactgct cggcctcgat ggcctaccat
      241 tcgggggagc ggatccgcaa gctcgactac gcggggttgc tgtgcggggc gctgtcgtac
      301 ctgatgctgc gccagcggga cgcggtcggg ctggtgacgt acgaccggaa aatccggcgg
      361 tacattccgc cgcggtcgcg gtcgggccat ctgcacgtgc tcctgaacga aatcgccgcg
      421 caggcgccct cggataccac cgatatcagt tcgacgctgc acgagatggc ggatcggatc
      481 aagcggcgcg ggctggtgat cattctgtcc gacctgatcg acgaggcgga caagctcatc
      541 tcgggactca agcattttcg ctacaacaac cacgaggtga tcgtcttcca cattctcgat
      601 ccccgcgagc gggattttgc gttcccgcgc gaggcggtgt tcaaggatat ggagacgggc
      661 gaggaactga cgacgctgcc ctaccagatc aagaaggatt atgcgaaggc ggtggcggcg
      721 ttttcgtccg aaatcgcctc cgcctgccgc caggccgcga tcgactatca cccgatcgac
      781 acggcgatgc cgttcgacaa ggcgctctat gcgttcttga gcaagcggga acggctgtac
      841 tgagcgcacg gcgaggggag cgggtgggga accaggatcg ggcgcaagga gaccgtgatc
      901 agggttgctg aaaatgcgcg gggaagatag gagcttgatc atcctttcaa cggcggggac
      961 gaaaaagtga cggctgtatg agtacggcgg cctggtcggt gctcattctg gcggcggtgc
     1021 ttatcgggca cctggtggag gaagcggcaa cgggattccg ccggaaattt ccgcttggtg
     1081 agatgccgat gtgggtcttt gtaatgggca atgtcgccgt gtacgggttt tgcgccgcga
     1141 cctttaccct cgtgctgctt gcacgtccgg cgggaacgct tatggcatgg gttctggcgg
     1201 ttgcgatggc actcaacggc atcgtgcata tcggcgtgat gatttaccgg aaggagtatt
     1261 ttcccggcgg gattacggcg ggcttggtgc tggcggcggc ggcgggattg atggcggcgc
     1321 tggcgcgggg gtaggaggca ggggggatcg gcgacgcaga cagaccccgc cgctgcggat
     1381 gagcactccg gacaccacag acctggctga gcgcacggcg ttctgggcga tcggggcagg
     1441 gcgcagcagg ccaattcggg cgcgcaagcg cttcaggcgt cccgtgcgga ggcgccgggg
     1501 gacggccggg cgaagaagag gcgcagcata taccacacca agcccagcgc cagcacgaaa
     1561 cagaccagga aaacgaggaa tacatcccac tgcggccgga tgggccaggc cgcggggtcg
     1621 aaatgcccct caagctggag gaggcggaca ttgtggcgga ccacgaccat cccgagcagc
     1681 gacacaaaca gcatcgcccc ggcgccgaag aatttcctcg tgaagaagaa atggagcgac
     1741 ccgagcgaga gaatgatcga tgcggagagc caccagatcg ccggcgtgtg catgaaaggc
     1801 ttgaggatgt ccgcgagcga caacaggtag acgagcccga ccgccatcgc ccccaccatg
     1861 ccccagagga agaagtttcg gccgagcgcg aaagcatcct cgtgccgccg cccgaggaga
     1921 ccgatgaaga atccgccgat ggtgacggcg ccgaggagca tgtggagcca gcgccagagc
     1981 cactggccga ggtgcgggtt gacccggaga ccggaagggt cgccggcgta aaggacggcg
     2041 gcgaggccgg gacgctcggc gatcgagaag aggttgctgt aggcaaacgc cacatacgcg
     2101 agcaagagga gcgcggcggt cagaaaggcg ggacgccggg gggagccggg cgccccgaag
     2161 gccgccgcgt agaggagata gtaggcaacc atgacggcgc cgacgatgcc gaggaagaac
     2221 caggcggtca cgatcgcggc gctgtacacg ggcacggggt acaccaactg cacgaacagc
     2281 aggggagcga cgccgagggt gacggtggcg gccatggcgg tgggaaacag tttgacgaac
     2341 tgcgcgacga cggggtggcg ccacttgtcg gcgaggcggc cgaaaacgac gatgaggaga
     2401 ccgccgacca ggaagttcat ggcggcgaag tggagggtga gggtgaggag gtgcaggaca
     2461 gtgaccagcc acagcggggc cgggagaaaa ttgggatcgg gcagcgggtt catcgggcgc
     2521 ctcctcgcgt tgcggccgcc ggcgtgccgg cgggcgcgct cagtgtctgc agaaactgaa
     2581 tgagcgcgac gcgctcgggt tcgccgccgg tgaagggggg catttcctcg ccgagatcat
     2641 cggacatgtc caggagtgtc cgatactcct cttcgctcag accggccagc gactgagtct
     2701 tgtcgttgaa gccgccgagg acgtgacagg aaccgcagcg gacctcgtag acgcggcggc
     2761 cgaggtcgac gccggagagc ccgcagacct cggcgaaggg ccggcggtcg gtgcgggcgg
     2821 cgaggtgggc ggccagcagc cgggcctcct cgggcgtgcc cgtccagggt ggcatgttgc
     2881 cgcgcatggc gccgatcccc tgcaccatgg cggtgatgaa ctcctcatcg gcgccgtcga
     2941 aaaacggctt cagcgcgttg tagttctcca gcgagtggca gctccggcag gcgcggcgga
     3001 aaagatcggc gccttcgtcg ccggtgcgat aagccatgtg cgcgagcatg ccgtcggcgc
     3061 gatagtcggc cgcccgggcg acctctaccc cgctcgcgta catgtagccg gtgatcacgt
     3121 agggcttgcg gagggcttcg cggaaccatt cgtacgagcc gaaccagacc aacccgaggg
     3181 ccagagcaac cgccgcgact tctgtgcgcc aggcccgggg agcgaggagt ccgaggatgg
     3241 ccagcagggc cgcgatcgcg gcggtcagaa gatagaggag atcgagggcg gcaaacggga
     3301 tgttcatcgt ctggcgggcg gccgccatga gatcggcggg gatgtcgccg acataccaga
     3361 agagggaggg gatgacgacc gcgagcccga cgaggcccca gaccgcattg tagcgcacca
     3421 ggcgcactcg cgcgccgggc gcgggaaggc gggcggccac cgcgagggcg cagaggccgg
     3481 cgagcatgat gcagatcccc gtgcgcagca cgagcgagga ccagtaggtc gggttgaaga
     3541 aaccgtccca gaatcggccg gtggccagcc agtcgcccgg ggtgagcatg aaggtcacga
     3601 tgccgttgat gatgaccagg gagagccagg cggcgccgaa atagatccac ccaatgatca
     3661 ggtggcggcg cggggtgagg cagtcccagc cgtagtagta gagcagggcg gcgaggatct
     3721 cgatgatgaa gaagcaccac tcggcggccc agccccagac gaaattgcgg atgaggaccg
     3781 cggtggcggc cgggttgagc agaccgatga tgaaccagat gccgaccccg gtgagggcgc
     3841 cgaagaccac cgtggtgagg acgaagaacc ggctcagccg ccgcaggaat ccgaggtggt
     3901 catcgtcgcc gcggcggcgg gccgcacgct cggccacgac caggtacaag ccgccgccga
     3961 tggcgaagtg agagacgaag acgtggacga ccgcgatcac ggccatgagc acgccgtaac
     4021 ccagggtcgt atcccagaag ggataattca tgcgttacct ccgcaagggc accggacaat
     4081 cttgtgaaat aaataacaaa acggaccgga agaatcaact atatttcccg caaggcccgg
     4141 ccagggcagc tccccgcggc taggtgtcgg ccgatttccg tttcgaaaaa tactcgatat
     4201 cgggggcgtg gtcatagccg agacgggcca cgagctccat ggaaggttcg ttccagtctt
     4261 cgatcaggca ggcgatgatc tcggctccgc gctcgtgcag gaattcttcg caggcgcgga
     4321 cgatccgggc ggccagaccc tggcgccgga agtcggggtg gaccgccagc cggttgatcc
     4381 agcccttgcg gccgtcgaac gtggccagcc cggcggcgac cagccggtcg cggtcgaaaa
     4441 cgccgaaaaa cgcggtgtcg gggcggcgca tctcgatttc gattctttcg cgggagtcgc
     4501 ggccgagagg ccggtagggc aggccggcat ccccccacag gttgatgagg gcgtcgtaat
     4561 cggcgatcgt cagtttcacc aggtgacggg tcatgagact tctccatcgt gacaggtcaa
     4621 ccggagcaag ataggcccga gggcgggaag gcgaaagtga gaatgcggcc gggggcggtt
     4681 acggcgacag ccgggcggcc cgcttttcct cgaggatctc tctcagccag gacatgggaa
     4741 aacgggagcc gaaggggacg tcgttccagt gttcgcagcc ggtgaagctg aggaggacgc
     4801 cgcgggcggg gatatcgagt tcggcgcagg tgcgcaggcc ggaggtgagc atgatcatgc
     4861 aggcgacgcc gacgacgccg acgttgctca gccgctcggc gtagtgttcg atctggttcg
     4921 agagcttgcg tttcgagaac gccacccggg cgccgtactc cccggccagc tccatcaccc
     4981 agtacgactg gcaggccggc tcgcactgct ggcacgtgtc gccgtactcc ccctcgacct
     5041 tccggcagtc ggggttgttc agcgagagac agtcgggcac gatgatgagc gtgtcgccgg
     5101 tccggttgaa ggcttcacgg ttgaggtcgt cgtagaacga gaaggcgaga gcctcgagga
     5161 ggtacttctc cttgggcgtg cggcggaggt ccttttcggg agagtcctcc gcccaggcgc
     5221 ggcagatgaa ttcgtcgagg ccggcgaact cggcggcgaa ttcggcgaaa ccttggtcga
     5281 caatgcggcg gcgaaagagg gccagtcttt ttcggaagtc cggcccgagg cggtaggttg
     5341 ggtaacggcg cgtcatcgcc agtcaatata cggactgacg gcggccgggc gcgagaaata
     5401 tgaggggggc ctagcgggcc gggcgctccg tcagccggcg gagaccgtcg cgggcatccg
     5461 acgactccgg gtgcagcgcc agcgcggccc ggtagctctg ttcggcgcgg ccgtagcggg
     5521 cctgggcttc gaaattgcgc gcccgcgcaa ggtgtatctg gctgcggatg cggctgtcgg
     5581 ggggcgccag gtcgaggctc tcggacagaa acgcctcggc cgtggcgtag tcctcggccg
     5641 cgcagcggat cagaccgaga gcgtagaacg gttcccagtg gtcgggatgc cgggccaccg
     5701 cccggcggag gaccgccacc gcctccgggt agcgctgctg gcggtagagc atgaaaccgt
     5761 cgctgacgag cgaccgaaac tgctcgtcgg tatcggccgg gaggacggcg gcggaatcgg
     5821 cggaatcggc ggcggtcggc gggccgtcga cgcggacggc cttggtcgtg accatgccgc
     5881 cgctgcagcc gcccaggacc aacacgagag tcgcggcggc gtacaagcgg gtgagggcag
     5941 gctggcgcat tgactttgtc ctcctgggga gcataggtcg aaatatcggc gcggccggcg
     6001 tgacgcctga gcgggggcgg gaaaaagggt tgaataaacc gctggattgt agataatatg
     6061 ggcaggaatc cgagcggctg atcaaagaga ggacaggtat ggcgaacgga acaacacccg
     6121 tgttctacaa gaatccgggg ctggcggcgg tgctgtcgtt cttctggatg gggctggggc
     6181 agatctacaa cggccagatc gccaagggcg tgtttttcat cgtcctgtac gcggtgtcgg
     6241 tgctcatgat gctgatcctg atcggctttc tcaccacccc ggttctgtgg atctggggaa
     6301 tgtacgacgc gtattcctcg gccgagaaac tcaaccggga gctggcgagc cgtcagggaa
     6361 gcgggccggc cgggtactga gggcccccgc ccgaggcggt caggtccgcc tccgggccac
     6421 gacacacacg cgcagggtcg accggtcggc gggcgtgaat cggtagcaat cgtagaagcc
     6481 gcggacggtg aaacccgcgg aacgcaggag ctgtcgaatc cgggtgttgc tgtaggcgcg
     6541 ttcgtagtgg atctcgtcga aacggtccca gcggtcgccg cgtttgacga aggtggcggc
     6601 ggtgaggcag gccgtgcggg accgggcctg gtactcgctt ttccacaccc aggcaagttc
     6661 ttcgcgggca tcggcgaaga cgtggccgct ccagatgtcg gacagggcgg cagccgtgtt
     6721 catgtcgaag atgaaccagc cgcggtcgtt gaggtggtcg tggaccgacc gaaatgccgc
     6781 gccgaggtcg cgttcggtca acaggtggtt gagcgaatcg tagaagcagg tgatgaagtc
     6841 gacgcgaacc gtgccgcgcg ggcggtcatc ggggatgagg cggaaagcgg ggagagtttt
     6901 ctggtagaga cggacacccc ggccgcggag tttgcgggcc gcctgggcga tcatctcggg
     6961 agagccgtca agcccggcca tgatgaaccc ccggtcgagg aagcgggcga gagcggtgcc
     7021 ggtgccgcaa cagaggtcga gaccggtgac ggcccggatg cgatggcgtt tcatgagcgc
     7081 cagggtgtag tcgaccatgc gggcggagtg gcggtcctga cccatcagat cccagacggc
     7141 ggccaggccg gcataggggg ccccgggcgt cactgacggc tctctcgtac agcgatcata
     7201 tcagcgcatg atagcgcgcc ggagtcgcac cgccaagcgg aaaggcgcgc cccagagggc
     7261 gggcgaccgc cgacaaaaat cacaatcttc cgcccgccgc ccgccgggag aaacatggta
     7321 ttgacgcatc cggagaagcg gagttattgt ggggggagaa ttcacgggga tgcgttcgcg
     7381 cggggggccg ccggtttttc cgtcggttcc gccgccgcaa acatgacgga tggaggtgtg
     7441 ggatgcgcct cgcggtcatt tccgatgtcc acctgggcga cccgatgtgc caggtggtgg
     7501 tcagggaggg ggggcgctgc cggccggggc cgcggctcga gcaattgcag gcggcgctcg
     7561 cgccgggcct cacctatctc gtcctgatcg gcgacatcct ggatttttcg ctcgcgacct
     7621 acgaagaagt ctacgaggcg gcggaggtgt ttttcggcgc cctggccggg acggcggagt
     7681 acttcgtcta cgtgccgggc aaccacgact tcgacgtctg gacgacggtc gagtaccagg
     7741 tgaacatcat caatcggctc gaccgcggga ag
//
LOCUS       DAOPOE010000124         7756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10495_length_7756_cov_5.474873, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000124 DAOPOE010000000
VERSION     DAOPOE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10495_length_7756_cov_5.474873"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1187)
                     /locus_tag="PKM94_12445"
     CDS             complement(252..1187)
                     /locus_tag="PKM94_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67485.1"
                     /translation="MNTTSGAIVLAAALLLGEPAAEAAPDGGGDSLTIPFRLEHSRII
                     IPSSVNGSRPLALILDTGMPIEGAYLFHEEFVSEIDTARAAVVRVPGAGAGEASTALM
                     VEDGVIACGGYTVTGQRIIVSRSAHTQGFGSDGVIGRSLFGRHTLEVDFDREVIILHD
                     TAVAAADSSWYAVPVTASNELPFLQGEIETVPGEVVPVTVYVDLASRDALELLMRPGQ
                     RWTMPDSAESSYLGTGLSGDISGYRGRVAKLRIGPYELSDVAAVFAPAEVRSKQKKAD
                     GILGCGLVMRFNVIFDLAHGVLYLKPSRHYGEPFR"
     gene            1353..1961
                     /locus_tag="PKM94_12450"
     CDS             1353..1961
                     /locus_tag="PKM94_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOD67486.1"
                     /translation="MTDDKLKAYREKRDFRKTAEPSGGPAAGRGQPLFVIQKHRASHL
                     HFDFRLEIDGVLKSWAVPKGPSANPSDKRLAVETEDHPIEYADFEGTIPEGEYGGGTV
                     MIWDAGRYANLTQDREGGEIPLAEAYARGAIEIELHGARLRGGYALRRFKKERDRGQW
                     LLIKMRDAHADPDRDPVAEHATSVKTGRTMEEIEHAEGGGEG"
     gene            2117..3229
                     /gene="hflX"
                     /locus_tag="PKM94_12455"
     CDS             2117..3229
                     /gene="hflX"
                     /locus_tag="PKM94_12455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOD67487.1"
                     /translation="MAFDVAKNNSEKAVLVGVAATSRDRVAVEESLDELAALTVSAGA
                     RVVGRRIQVRPAPDPATYIGKGLVEELKNLLTDLGGNCLIFDEPLSPAQQRNLEERLE
                     AKVIDRPILILDLFATRARTAAARLQVELAQLEYTLPRLTGAWVHFSRQYGGIGAKGP
                     GETQLEIDRRQIRTKIAHLKQRLEKLGTQRAEQRKGRRELFKVALVGYTNAGKSTLFN
                     RLTKADVRTADMLFTTLDSTTRVMSAGYPCRIVFSDTVGFIKKLPHQLVASFKSTLEE
                     VATADLLLHVVDVADPHVDEHIEHTRRVLAEIGAEKIPYLMVYNKIDAKPDSCAANGN
                     HDRTFFISALEGRGLAALQAELIARSSGRPRESSTD"
     gene            3282..3635
                     /locus_tag="PKM94_12460"
     CDS             3282..3635
                     /locus_tag="PKM94_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD67488.1"
                     /translation="MAKPILIVDDNPNMSSLLSDMLEVFDYPSERAGDGQEALDKLTA
                     QPFSMVITDMRMPNMTGLELVGRIKQMYPKLPVVLISGYAMSELDHLDGPRPDGFLAK
                     PFMMSDVERLINSLL"
     gene            3810..4499
                     /locus_tag="PKM94_12465"
     CDS             3810..4499
                     /locus_tag="PKM94_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD67489.1"
                     /translation="MRALLKMSQLFAGLDDASLAEVETATSLRRAVKGEILFSEGDPA
                     ASFFVVGEGKVKVFKLSPDGKEQILMIAKPGDTFAEAAIFNDGRYPADAEAIEDTELL
                     VVNRDRFIALLGRNPNLAFSLIARLCALLRKMATLVEGLALTDVTTRIAHYLARQADR
                     VLKKQPDRAGANAVTVTLPEKKAVLAAQLGTIPETLSRSLAKLTREGIIEVDGPRIHI
                     KDLSRLRGTFE"
     gene            complement(4521..5381)
                     /locus_tag="PKM94_12470"
     CDS             complement(4521..5381)
                     /locus_tag="PKM94_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOD67490.1"
                     /translation="MWQWLRHYFGGLYDRSDSHHLFLNASGLAFSLFVCVVPMVLILF
                     YVLGTVLERKQVERELAAWIDRVIPYEKYTEEPKQFIFSRLDEFRAYRKVAGVVGALG
                     LLFAASGLFSSMRTVLNQIFHTSVSKHVLIGKLRDLGMIVLVIGYFLLSMLTLPILDV
                     LAREASGIGMLKSLNLSTLDQVLVTVLSFAVIYVAFWSLYHFIPYARMERRVVAVSAF
                     WAALLWEVAKQAFSVYLSNAATLPVIYGTYLFIIVAAFWIYYSSIVFILGAEIGQLYR
                     ERREAKTGTG"
     gene            complement(5520..6698)
                     /locus_tag="PKM94_12475"
     CDS             complement(5520..6698)
                     /locus_tag="PKM94_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67491.1"
                     /translation="MSANRVPDGDGVIDAHVHVGLRDGWNGWGRMSAAYASEANLTFR
                     IFALYAGLAGEAITDERLVAAAIDYALSAPVRSVVMLALDHVYERDGRPKPERSHLWV
                     HNDYILYLRAEVARRGQPGKVLFGASVHPYRTDFAERVRECADKGAVLLKWVPSAQDI
                     DLASETVAERLEFLAAFRKPNGKPLPLLLHVGPEYAIPPADPAGTSYDFLTWTAGDRF
                     WNRVLSRLGVRPRWRDRDGAKIRRHLARALDAGAVIIFAHCGLPYWSPKFAQRWFEKS
                     EFETVKGYLAENGAPGRRGKAYADLAAFCTPFRQGYFAQAARLPQEYLLFGSDAPTPV
                     FDLGADPGAVWEGFRGVLKGDFAKAVVPAGNASGVNYRVLRRFFPEARVFGENFARLI
                     "
     gene            complement(6695..>7756)
                     /locus_tag="PKM94_12480"
     CDS             complement(6695..>7756)
                     /locus_tag="PKM94_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67492.1"
                     /translation="LDRGKPVRPFRWTVPAVFDDRPGRRDRQVLPTVDPSPEGPPYGG
                     LFLDALGTGGGGRSRFIFAYPNAYLATADGELIMMTHGHYFEWYWSALYEAVTGIVAG
                     HPPGAGEAEPADPTLEQMVAVNSTACQLSCSGIGQAGLLSRVINEVVAEVKVESRHRS
                     LAATSRYLDNAIAYVDAYTRIGGDLFDRLDLDERVMEAIKARLMRKLRAYEAARHRVP
                     FLRNKGTRERFGAYFAAGAREIAALNERHACALPESPGAVIFGHTHRPLRWEPTRVPN
                     ADYDYPYRGRTVRLFNAGAWINREREGRGQVFSGAEVFLYDSDIPGGMTSVAIEAEAG
                     PGPEMIADGPGASDGPEGS"
BASE COUNT         1400 a         2857 c         2301 g         1198 t
ORIGIN      
        1 tggggcgggg aaggggtcaa actcgtgcag cagcgccttg agggcatgaa gatcaagacc
       61 ctcgacgaga tcttcaaagc ccgtctcgtg ccgtcgtccg aagacatggc cagtctcgac
      121 gagtgggtgg gcaagttcgc cgcgctgatc gaagagaagg cggccggcgg cgagtgaccg
      181 cggaactctc gaaccggccc ggccggcgga tgcccgcccg ccggggccga acccggtcgg
      241 ggtagcggcg ctcagcggaa cggttcgccg tagtgccggc tcggcttgag gtacagcacg
      301 ccgtgcgcca ggtcgaatat cacgttgaac cgcatcacca ggccgcagcc caggatcccg
      361 tccgccttct tctgctttga tcgcacctcg gccggcgcga acaccgccgc cacgtccgac
      421 agctcatacg gcccgatgcg cagcttggcc acccgacccc gatagccgga gatgtcgccg
      481 ctgagaccgg tcccgaggta ggaggattcc gcgctgtccg gcatcgtcca ccgctggccc
      541 ggccgcatca gcagctcgag cgcatccctc gacgcgagat ccacatagac cgtgaccgga
      601 acgacctccc ccgggacggt ctcgatctcc ccctgcagaa agggaagttc attcgaggcg
      661 gtgaccggca cggcgtacca cgaactgtcg gccgcggcga ccgctgtgtc gtggaggatg
      721 atgacctcgc ggtcgaaatc aacctcgagc gtgtggcggc cgaacaggct ccggccgatc
      781 actccgtccg aaccgaaccc ctgcgtgtgc gcgctgcgcg acacgatgat tcgctggccg
      841 gtgaccgtgt acccgccgca ggcgatcacc ccgtcctcga ccatcagcgc ggtcgaggct
      901 tctcccgcgc ccgcgcccgg aacccggacc accgccgccc gagcggtgtc gatctccgag
      961 acaaattcct cgtggaacag gtacgccccc tcgatcggca tcccggtatc gaggatcagc
     1021 gccagcgggc gagaaccgtt gacgctcgag ggaatgatga tccgggaatg ctcgagccgg
     1081 aagggaatgg tcagggaatc cccgccgccg tcgggcgcag cctcggccgc cggttccccc
     1141 aggaggagcg cagccgcgag aacgatcgcg ccggatgtcg tgttcatatc accgccttcg
     1201 tttggaccgt ctggactttc tccgggacgt tcgggatcct ctcaatgtgc agcgccgccg
     1261 cgccccgaac caccgtaatc tttcccgcgg aaactttccc cgggcccccc ggttcactat
     1321 aatactgagc ggcgccgcaa gccggaggac ctgtgaccga cgacaaactc aaagcctacc
     1381 gcgagaaacg cgattttcgc aagaccgccg aaccatcggg cggcccggcc gccggccgcg
     1441 gccaacccct tttcgtcatc cagaaacacc gcgcctccca cctccatttt gacttccgcc
     1501 tcgagatcga cggcgtcctg aaatcgtggg cggtgccgaa aggcccctcg gccaacccgt
     1561 ccgacaagcg cctcgccgtc gaaaccgagg accacccgat cgagtacgcc gacttcgaag
     1621 ggaccatccc cgagggggag tatggcggcg gcacggtcat gatctgggac gccggccggt
     1681 acgccaatct tacgcaggac cgggagggcg gtgaaattcc cctcgccgag gcctacgcgc
     1741 ggggcgcgat cgagatcgaa ctccacggcg cccggctccg gggcggctac gccctgcgcc
     1801 gcttcaagaa ggagcgcgac cgcgggcagt ggctcctgat caagatgagg gacgcgcacg
     1861 ccgaccccga ccgcgacccc gtcgccgaac atgccacctc ggtcaagacc ggccgcacga
     1921 tggaggaaat cgagcacgcc gagggcgggg gagagggatg accgtcccgc acgccgccgc
     1981 cacgagtcgc ggaggggcca ggggggggac gagcgctgct cccacccgct ttctctgttg
     2041 ccaaatccgc cgttgcgcat ataattgata aaggacgaaa tcagtcaaca acgcccggag
     2101 accgtaacaa cccttagtgg cattcgacgt cgcaaagaat aattccgaga aggcggtcct
     2161 cgtcggggtc gccgccacct cccgcgaccg ggtggcggtt gaggaatcgc tcgatgagct
     2221 cgccgccttg acggtctccg cgggcgcccg ggttgtcgga cggcgcattc aggtccgtcc
     2281 cgcccccgat cccgccacct acatcggtaa aggccttgtc gaggagctca agaacctcct
     2341 taccgacctc ggcggcaatt gcctgatttt cgatgaacct ctctcgccgg cgcagcagcg
     2401 caacctcgag gagcgcctgg aggcgaaagt catcgaccgg cccatcctca tcctcgacct
     2461 gtttgctacc cgcgcccgca cggcggccgc gcgcctccag gtcgaactcg cccagttgga
     2521 atacaccctc ccgcgtctca ccggcgcctg ggtgcacttc tcgcgccagt acggcgggat
     2581 cggcgccaag ggccccggcg agacccagct cgagatcgac cgccgccaga tccggaccaa
     2641 gatcgcccac ctcaaacagc gcctcgagaa actcggcacc cagcgcgccg aacagcgcaa
     2701 gggccgccgc gagctcttca aagtggctct ggtcggctat accaacgccg gcaaatcgac
     2761 tctcttcaac cggctgacca aggccgatgt ccgcaccgcc gacatgctct tcaccactct
     2821 cgactccacc acccgcgtca tgtccgccgg ttatccctgc cgcatcgtct tctccgacac
     2881 cgtcggcttc atcaagaaac ttccgcatca gctcgtggcc tcgttcaagt cgacgctaga
     2941 ggaggtggcc accgccgacc tcctgctgca cgtggtcgac gtggccgacc cccacgtgga
     3001 cgagcacatt gagcacaccc ggcgggtgct ggccgagatc ggcgccgaaa agattcctta
     3061 ccttatggta tacaacaaga tagacgcaaa gcccgacagc tgtgccgcca acggcaacca
     3121 tgatcggacc ttttttatct ccgctctgga aggccgcggg ctggccgccc tgcaggccga
     3181 actcatcgcc cgctcctccg gccgcccccg cgaatcttcg acagattagg tcaaggctta
     3241 cataactcct gccgatactg tgcctaagga gtaggcaccg tatggcaaaa ccaattctca
     3301 ttgtcgatga caacccgaat atgtcatcgc tcctgtcgga catgctcgaa gtcttcgact
     3361 atccctcgga gcgcgccggc gatgggcaag aggcgctgga caagctcacc gcccagccgt
     3421 tttccatggt catcaccgac atgcgcatgc cgaacatgac cggactcgaa cttgtcgggc
     3481 ggatcaaaca gatgtatccg aaacttccgg ttgtgctgat ttcgggctat gcgatgagcg
     3541 agctcgacca tttggacggt cccaggccgg acgggtttct ggccaaaccg ttcatgatgt
     3601 cggatgtcga gcggctcatc aattccctgc tttagatcca cgcgattctg gataccatcc
     3661 gccactagta atcccgggcc ccgcaccact cggtgtgggg tttttattgg ccgcacgggg
     3721 tccgcccggc cccgcccttt tcggtttaca gacacccctg cactagctac cttgcttgcg
     3781 ccccttcatg tggagaattg gagccgcccg tgcgtgcgtt actgaaaatg tcccagctct
     3841 ttgccggtct cgacgacgcc tcgctggccg aggtcgagac cgccacctcc ctccgccgcg
     3901 ccgtcaaggg ggaaatactc ttcagcgagg gggatccggc cgcctcgttt ttcgtggtgg
     3961 gggaaggcaa ggtcaaggtg ttcaagctct cgcccgacgg caaggagcag atcctcatga
     4021 tcgccaagcc cggggatacc tttgccgagg cggccatctt caacgacggc cgctacccgg
     4081 ccgacgccga ggcgatcgag gacaccgagc tgctggtggt caaccgcgac cgcttcatcg
     4141 cgctgctcgg ccgcaacccg aacctcgcgt tcagtctcat cgcccgcctg tgcgccctgc
     4201 tccggaaaat ggccaccctg gttgagggcc ttgccctgac cgacgtcacc acccggatcg
     4261 cccactacct cgcccgtcag gccgaccgcg tcctgaaaaa acagcccgac cgcgcgggcg
     4321 ccaacgccgt caccgtcacc ctccccgaaa agaaggccgt cctcgccgcc cagctcggga
     4381 ccatccccga gacgctctcc cgatcgctgg ccaagctcac gcgcgagggc ataatcgagg
     4441 tcgacgggcc gcgcatccac atcaaggacc tctcgcgcct ccgcgggacg ttcgagtagg
     4501 ctgcgccccc gcccgccccg tcacccggtc cccgtcttcg cctcccgccg ctcccggtag
     4561 agctgcccga tttccgctcc caggatgaac acaattgagg agtagtaaat ccagaaggcg
     4621 gcgacgatga tgaacaggta ggtcccgtaa atgaccggga gcgtcgccgc gttgctcaaa
     4681 tacaccgaga acgcctgttt cgccacctcc cacaggagcg ccgcccagaa cgcgctcacc
     4741 gccaccaccc gccgctccat ccgcgcgtac gggatgaagt ggtagaggct ccagaaagcc
     4801 acatagatca ccgcaaacga cagcaccgtc accagcacct gatccagcgt gctcaggttc
     4861 agcgacttca gcatgccgat accgctcgcc tcccgggcca gcacgtccag aatcggcagg
     4921 gtgagcatcg acaagagaaa atagccgatc accagcacga tcatcccgag gtcccgcagc
     4981 ttgccgatca gcacgtgctt ggacaccgac gtgtggaaga tctggttgag caccgtgcgc
     5041 atcgaagaga acagcccgct cgccgcaaac agcagcccca gcgcgcccac cacccccgcc
     5101 accttccgat acgcccggaa ctcgtcgagc cgcgaaaaga tgaactgctt cggctcctcc
     5161 gtatacttct cgtatggaat cacgcggtcg atccaggccg ccagctcccg ctccacctgc
     5221 ttccgctcga gcacagtccc cagcacgtag aacagaatca gcaccatcgg caccacgcag
     5281 acgaagagcg agaaggccag cccgctcgcg ttaaggaaga ggtggtggct gtcgctgcga
     5341 tcgtacaacc ccccgaaata gtggcgcagc cactgccaca ccgaccggcc gctcggcagc
     5401 cacgggacgg tggaagaggc cgcggcgggg ggtcggcggt ctgtctgtat cggtcgccat
     5461 cagccggtaa ttagccgcct cgccgccccg ctgtcaacaa cccccgcgcg cggcgccggt
     5521 cagatcaggc gcgcgaagtt ctccccgaac acccgcgcct ccggaaagaa ccgcctcagc
     5581 acccggtagt tgacgccgct ggcgttcccc gccggaacca ccgctttcgc gaaatccccc
     5641 ttgagcaccc cccggaaccc ctcccacacc gccccgggat cggccccgag gtcaaatacc
     5701 ggcgtcggcg cgtcgctccc gaacagcaga tactcctgcg ggagccgcgc cgcctgggcg
     5761 aagtacccct gccgaaaggg cgtgcagaac gccgcgaggt cggcgtatgc tttccccctg
     5821 cgcccgggcg cgccgttctc cgcgagatag cccttcacgg tttcgaactc cgacttttcg
     5881 aaccaccgct gcgcgaactt cggcgaccag taggggagac cgcagtgggc gaaaatgatc
     5941 accgcccccg cgtcgagcgc ccgcgccaga tgccggcgga tcttcgcccc gtcccggtcg
     6001 cgccagcgcg gccgcactcc cagccggctc agcacccggt tccagaagcg gtcgcccgcc
     6061 gtccaggtga ggaagtcata cgacgtcccc gcgggatcgg ccggggggat cgcatactcg
     6121 ggcccgacgt gcaaaagaag cggcagcggc ttgccgttgg gcttgcgaaa cgccgcgagg
     6181 aactccagcc gctccgccac cgtctcgctc gccaggtcga tatcctgcgc cgacggcacc
     6241 cacttcagga gcaccgctcc cttgtcggcg cactcccgca cccgctcggc gaaatcggtg
     6301 cggtagggat gcacggaggc cccgaacagc acttttcccg gctgtccccg ccgcgccacc
     6361 tccgcgcgca ggtacaggat gtagtcgttg tggacccaca ggtgggaacg ctccggcttg
     6421 ggccgtccgt cgcgctcgta cacatggtcg agcgccagca tcactacgct tcgcaccggc
     6481 gcgctcaggg cgtagtcgat cgccgccgcc acgagccgct cgtcggtgat cgcctccccc
     6541 gccaatcccg catagagcgc aaagatccga aaggtcaggt tcgcctccga ggcgtaggcg
     6601 gccgacatcc gcccccaccc gttccagccg tcgcgcaggc cgacgtgcac gtgggcgtcg
     6661 atgacgccgt cgccgtccgg cactctgttc gcgctcacga tccctccggc ccgtccgaag
     6721 cgccgggacc gtccgcaatc atctccggcc ccggccccgc ctccgcctcg atcgccaccg
     6781 aggtcatccc gccgggaatg tccgagtcgt acaggaacac ctccgccccc gagaacacct
     6841 gcccgcggcc ctcccgctcc cggttgatcc acgctccggc gttgaacagc cggaccgtcc
     6901 ggccccgata ggggtagtca tagtccgcgt tcggcacgcg cgtcggctcc caccgcaggg
     6961 gccggtgggt gtggccgaaa atcacggcgc ccggcgactc cggcagcgcg cacgcgtgcc
     7021 gctcgttgag ggccgcaatc tccctggcgc cggccgcgaa atacgccccg aaccgctccc
     7081 gcgtcccctt gttgcggaga aaggggacgc ggtggcgcgc cgcttcgtac gcccgcagct
     7141 tccgcatcag tcgcgccttg atcgcctcca tcacccgctc gtcgagatcg agccggtcga
     7201 acaggtcgcc gccgatccgc gtatacgcat cgacataggc gatggcgttg tccaggtagc
     7261 ggctagtcgc cgccagcgac cggtggcggc tctccacttt gacctccgcc accacttcat
     7321 tgatcacccg gctcagcagc cccgcctgcc cgatccccga gcacgacaac tggcaggcgg
     7381 tcgagttcac cgccaccatc tgctccagcg tcgggtccgc cggttccgcc tccccggcgc
     7441 ccggcggatg cccggcgaca atccccgtga ccgcctcata gagggcgctc cagtaccact
     7501 cgaaatagtg cccgtgggtc atcatgatga gttccccgtc cgcggtcgcc aggtaggcat
     7561 tggggtaggc gaaaatgaac cggcttcgtc cgccgccgcc cgttcccagc gcgtcgagga
     7621 acagcccgcc gtagggcgga ccctccggac tcgggtccac cgtcggcaac acctggcggt
     7681 cgcgccgccc gggccggtcg tcgaacaccg ccggcaccgt ccaccgaaac ggccgcaccg
     7741 gcttcccgcg gtcgag
//
LOCUS       DAOPOE010000125         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10523_length_7740_cov_4.638907, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000125 DAOPOE010000000
VERSION     DAOPOE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10523_length_7740_cov_4.638907"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="rpsS"
                     /locus_tag="PKM94_12485"
     CDS             complement(<1..262)
                     /gene="rpsS"
                     /locus_tag="PKM94_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD67493.1"
                     /translation="MPRSLKKGPFVDEHLQKKVEALNRSGEKRVIKTWSRRSTILPDF
                     VGHTLAVHNGNKFIPIYVTENMVGHKLGEFAPTRTFRGHGGKL"
     gene            complement(285..1115)
                     /gene="rplB"
                     /locus_tag="PKM94_12490"
     CDS             complement(285..1115)
                     /gene="rplB"
                     /locus_tag="PKM94_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD67494.1"
                     /translation="MPVKKFRPITPSQRFRTVPTFEEVTSTMPEKSLLEPLRKSGGRN
                     NKGRVTAYCRGGGHKRHYRVIDFKRNKHDIPARVASIEYDPNRSARIALLHYADGEKR
                     YILAPQGLEVNMRVESGPAADIRVGNALPLERMPLGTNVHNVEMRPGKGGQMVRSAGS
                     FAQLVARDGNKAILKMPSGEVRSVPVTCCATIGQVSNVDHKNVVWGKAGASRWRGRRA
                     SVRGVAMNPVDHPMGGGEGRSSGGRHPCTPWGKPTKGYKTRSKRKSKDHLIQDRRSKV
                     "
     gene            complement(1142..1435)
                     /locus_tag="PKM94_12495"
     CDS             complement(1142..1435)
                     /locus_tag="PKM94_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD67495.1"
                     /translation="MSRDPRQVIKLHIQTERTTGLRQANNEYVFEVDKKANKHVIKAA
                     VEKAFKVKVDDVRTLIVPGKTRRMGRYEGKTPTWKKAIVRLKKGESISMFENI"
     gene            complement(1432..2055)
                     /gene="rplD"
                     /locus_tag="PKM94_12500"
     CDS             complement(1432..2055)
                     /gene="rplD"
                     /locus_tag="PKM94_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOD67496.1"
                     /translation="MNVKVYNQEGREVGTVELNPEVFGIKPNEAVVHQYVVNYLARQR
                     QGTVLTKTRKEVSGGGKKPWRQKGTGRARAGTIRSPLWRGGGTVFGPQPRTYGSRFPK
                     QMKKLAMQSVFSDKAALERIRVLDQVQLEKPKTKAVADLMAKLNLAGKKCLVLHEGRH
                     DNFMLSCRNLPAVKYCRAALANGYDVLDADYLVFTKAGLEKVAEVFA"
     gene            complement(2067..>2453)
                     /gene="rplC"
                     /locus_tag="PKM94_12505"
     CDS             complement(2067..>2453)
                     /gene="rplC"
                     /locus_tag="PKM94_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOD67497.1"
                     /translation="VQCAEAGLSVGAVLKADLFKPGDRVDVTGVSRGLGFQGAMRLHN
                     FNGANVTHGQSDRWRAPGSVGASSYPSRVFKGQRMAGRMGRNKVTALNLRVVRVYPEE
                     NLLLVRGSVPGHRGTLLRIRTTNRGK"
     assembly_gap    2454..2463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2464..2712)
                     /gene="rplC"
                     /locus_tag="PKM94_12510"
     CDS             complement(<2464..2712)
                     /gene="rplC"
                     /locus_tag="PKM94_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933842.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOD67498.1"
                     /translation="MREILGKKIGMTRVFEPSGESVPVTVIQAGPCPVVAVRTAATHG
                     YEALQVGFDEVKKERVNKPETGHFAKAGIAPTRYLQEVQ"
     gene            complement(2730..3038)
                     /gene="rpsJ"
                     /locus_tag="PKM94_12515"
     CDS             complement(2730..3038)
                     /gene="rpsJ"
                     /locus_tag="PKM94_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOD67499.1"
                     /translation="MNVQKIRIRLKAYDHYALDRSTKEIAQTVLRTGARIVGPVPLPT
                     KRTVYTVLRSPHVDKKSREQFETRVHKRLIDIYESTPQTVDALMKLDLPAGVDVEIKT
                     "
     gene            complement(3054..4262)
                     /gene="tuf"
                     /locus_tag="PKM94_12520"
     CDS             complement(3054..4262)
                     /gene="tuf"
                     /locus_tag="PKM94_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD67500.1"
                     /translation="MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAMTMVLSRVTGTQV
                     RSFDSIDNAPEERERGITIATAHVEYESARRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKVDQVDDPELLDLVELEVRDLLSQ
                     YEFPGDDVPVIRGSALQAMTVGSDLSKPLDSAEFKSIMELMEALDTYIPEPKRDKEMP
                     FLMPIEDIFSITGRGTVATGRIERGVVKQGEEVEIVGIRETRKTVVTGVEMFRKLLDS
                     GQAGDNVGLLLRGVDKEEVERGMVLAKPGSITPHKKFKAEVYVLTKEEGGRHTPFFSG
                     YRPQFYFRTTDVTGVATLPENVEMVMPGDNVQMTVELITPIAMEKELRFAIREGGRTV
                     GAGVVAEIVE"
     gene            complement(4293..4365)
                     /locus_tag="PKM94_12525"
     tRNA            complement(4293..4365)
                     /locus_tag="PKM94_12525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4330..4332),aa:Thr,seq:ggt)
     gene            complement(4769..4840)
                     /locus_tag="PKM94_12530"
     tRNA            complement(4769..4840)
                     /locus_tag="PKM94_12530"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4806..4808),aa:Gly,seq:tcc)
     gene            complement(4899..5765)
                     /locus_tag="PKM94_12535"
     CDS             complement(4899..5765)
                     /locus_tag="PKM94_12535"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOD67501.1"
                     /translation="MRLGLFANVTRQGAREAIQTFVEWAQQARHEVVLSTDLAGYTGD
                     HLTYLPRSDIAGYSNVIVSMGGDGTMLATARDIRDSGTPILGINLGSLGFLTQLTPKQ
                     LVAALDRIAAGEYLIEERMLLKTAVAGDHQPSWPYALNDIVVDKGAVSRLIELYFRVN
                     GEDIVTYRADGIVIATPTGSTAYSLAVGGPILHPKMQGIVVAPISSFSLTTRPMLFSA
                     DDVLEVTVKSPDRVVSLTLDGQVMTELFDNSRVTITRAEFTLKFIVFPENSFYKVLRN
                     KLHWGRPPRTGS"
     gene            complement(5829..>7740)
                     /gene="dxs"
                     /locus_tag="PKM94_12540"
     CDS             complement(5829..>7740)
                     /gene="dxs"
                     /locus_tag="PKM94_12540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOD67502.1"
                     /translation="EEIRQAIISSVSKTGGHLATNLGSVELTVALHYVLNLPHDRLIF
                     DVSNQTYTHKILTGRRDRMDTLRQYKGLAGFAKREESEYDHFGAGHASTSISAALGFA
                     AARDQKGEDRRVIAVIGDGAMTGGLAYEGLNNAGSLKKDLLVVLNDNTWSISKNVGAI
                     SRYFTSIMTDEKFNRLRNEIWELTGRFKRRDRIREAISRIEDSVLGLITPGMLFEKLG
                     FRYFGPIDGHDLELLIKRLRDLQNIRGPLLLHVVTQKGRGFRPAEGDPSRFHGVGQFD
                     KVTGKAAAKSAGLPTFTKVFGDTMVELGAKDRRVAAISAAMCDGTGLVDFAAAYPERF
                     FDVGIAEEHAGTFAAGLAAEGIKPYLAIYSTFLQRTYDQVVHDIALQNLPVVMCLDRA
                     GLVGNDGPTHHGVFDLCYLGSVPNVAVAAPKDGNELRAMLHYTAGHDLPGPVAIRYPR
                     GAVPTAMRPEIDPLEWGKWEYVTPRREVAVLAVGTMVAQAQEAAARMREGADRVGIIN
                     ARFVKPWDEAMLLEIADHARAIITMEEGQLRGGFGEAVGAFLLQRGWDGQFRALGIPD
                     RFVTHGDREELLAEIGLDAEAVATLLENVAREQGHEHGRGILRKLGLRRAMRFGHARR
                     KTEEEFSGAAEE"
BASE COUNT         1287 a         2825 c         2126 g         1492 t
ORIGIN      
        1 ccagcttccc gccgtggccc cggaacgtcc gcgtcggcgc gaactcgccc agcttgtgcc
       61 cgaccatgtt ctcggtcacg tagatgggga tgaacttgtt gccgttgtgc acggcgagcg
      121 tgtggccgac gaaatcggga aggatggtgg aacggcgcga ccaggtcttg atcacccgct
      181 tctcgccgct ccggttgagc gcctccacct ttttctgcag gtgctcgtcg acaaacggtc
      241 cttttttcag tgagcgaggc atctatcgtt ctccctacct tggcttacac tttcgagcgc
      301 cgatcctgga tcaggtgatc cttcgatttg cgcttgctgc gggttttgta ccccttggtc
      361 ggcttgcccc agggcgtgca ggggtgccgc ccgccggaac tccgtccctc gcccccgccc
      421 atcgggtggt ccaccgggtt catggccacg ccgcgcacgg aggcccgccg gccgcgccag
      481 cgcgacgccc ccgccttgcc ccacacgacg tttttgtggt cgacattcga cacctggccg
      541 atcgtcgcac aacacgtcac cggcaccgac cgcacctcgc ccgagggcat cttcaaaatc
      601 gccttgttgc cgtcgcgcgc caccagctgg gcaaacgaac cggccgaccg caccatctgg
      661 ccgcccttgc cgggccgcat ctcgacgttg tggacgttgg tgccgagcgg catccgctcc
      721 agggggagag cattccccac ccggatgtcg gccgccggac ccgattccac ccgcatgttc
      781 acctcgagcc cctggggcgc gagaatgtac cgcttctcgc cgtcggcata gtgcagcagc
      841 gcgatccggg ccgaacggtt cggatcatac tcgatcgagg ccacgcgcgc gggaatatcg
      901 tgcttgttcc gcttgaagtc aatgacccgg taatgccgct tatgtccgcc gccccggcag
      961 taggcggtca cccgcccctt gttattgcgc ccgccggact tccgcagcgg ctccagcagc
     1021 gacttctccg gcatcgtcga cgtcacttcc tcgaacgtcg gcaccgtgcg gaagcgctgc
     1081 gacggcgtga tcggacgaaa tttctttacc ggcataacgt gctaccaacc ttgtcttgtc
     1141 gctaaatatt ctcgaacatc gaaatcgact cgcccttctt caggcgcacg atcgctttct
     1201 tccacgtcgg ggttttcccc tcgtaccggc ccatgcggcg ggtctttccc ggcacgatga
     1261 gcgtgcggac gtcgtccacc ttcaccttga acgccttctc caccgccgcc ttgatcacgt
     1321 gcttgttcgc tttcttgtcg acctcgaaca cgtactcgtt gttggcctgg cgcagacccg
     1381 tggtccgctc cgtctggatg tgcagcttga tcacctgccg cggatcccgg ctcatgcgaa
     1441 cacctccgcc actttctcca gcccggcctt cgtgaacacc aggtagtccg cgtcgagcac
     1501 gtcgtacccg ttggcgagcg ccgcccggca gtatttcacc gccggcaggt tgcggcagga
     1561 gagcatgaag ttgtcgtgcc ggccctcgtg gagcaccagg cacttctttc ccgcaaggtt
     1621 cagtttggcc atcaggtcgg ccaccgcctt ggtcttgggt ttctccaact gcacctggtc
     1681 gaggacacgg atgcgctcga gcgccgcctt gtccgaaaac accgactgca tcgcgagctt
     1741 cttcatctgc ttcgggaacc gcgagccgta ggtccggggc tgcggcccga agaccgtgcc
     1801 gcccccccgc cacagaggcg agcggatcgt gcccgcacgc gcccggccgg tccccttctg
     1861 ccgccacggc ttcttgccgc cgccggagac ttccttgcgc gtcttcgtca gaaccgtccc
     1921 ctgccgctgc cgcgccaggt agttcaccac gtactggtgc accaccgcct cgttcggctt
     1981 gatcccgaag acctcggggt tgagttcgac ggtgccgact tcccggcctt cctgattgta
     2041 gactttcacg ttcatgtccg tatccgtcac ttaccgcgat tggtcgttcg aatccgaagc
     2101 agcgtgccgc gatgacccgg cacggagccc cgcactaaca gcagattttc ttcgggatag
     2161 acgcgtacga cgcgcaggtt gagcgccgtg actttgttcc ggcccatgcg ccccgccatg
     2221 cgctgtccct tgaacacgcg cgaggggtag gaggaggcgc ccaccgagcc cggggcccgc
     2281 cagcggtccg actgcccgtg ggtcacgttg gcgccgttga agttgtgcag gcgcatggcg
     2341 ccctggaatc cgagtccgcg cgacacgccc gtcacgtcca cccggtcccc cggcttgaac
     2401 aggtcggcct tcaacaccgc gcccacgctc agcccggcct ccgcgcactg cacnnnnnnn
     2461 nnnctgcact tcctgaaggt agcgcgtcgg ggcaattccg gctttcgcaa aatgcccggt
     2521 ctccggcttg ttcacccgtt ccttcttcac ctcgtcaaaa ccgacttgga gcgcctcgta
     2581 gccgtgggtg gcggccgtcc gcacggccac gaccgggcac ggcccggcct ggatcaccgt
     2641 caccggcacc gactcgcccg acggctcgaa cacgcgggtc attccgatct tcttgcccag
     2701 tatctctctc attgccgtca gccttcccgt caggttttga tctccacgtc gacacccgcc
     2761 ggcaggtcaa gcttcatcag cgcgtccacc gtctgcggcg tggactcata gatgtcgatg
     2821 agccgcttgt ggacacgcgt ttcgaactgc tcgcgcgatt tcttgtcgac atgcggggac
     2881 cgcaggaccg tgtagacagt ccgcttcgtc ggcagcggca ccgggccgac aatccgcgcc
     2941 ccggtgcgga gcaccgtctg cgcgatctct ttcgtcgacc ggtccagggc gtagtggtcg
     3001 tacgctttca gccgtatccg tattttctgc acgttcatat tcgcttcaac ctgttactcg
     3061 acaatttcgg cgacgacgcc ggcgcccacc gtccgaccac cctcccgaat cgcgaaccga
     3121 agctccttct ccatcgcaat cggcgtgatc aactccaccg tcatctgcac gttgtccccc
     3181 ggcatcacca tctccacatt ctccggcaac gtcgccaccc ccgtcacgtc cgtcgtccga
     3241 aaataaaact gcggccgata cccgctgaaa aacggcgtgt gacgaccccc ctcctccttc
     3301 gtcaacacgt acacctccgc tttgaacttc ttgtgcggcg tgatcgatcc cggtttcgcc
     3361 aacaccatcc cccgctccac ctcctccttg tccacccccc gcaacaacaa ccccacgttg
     3421 tcccccgcct gacccgaatc caacaacttc cgaaacatct ccacccccgt caccaccgtc
     3481 ttgcgcgtct cccgaatccc cacaatctcc acctcctccc cctgcttcac caccccccgc
     3541 tcgatcctcc ccgtcgccac cgtcccccga cccgtaatcg aaaaaatgtc ctcgatcggc
     3601 atcaggaacg gcatctcctt gtcccgcttc ggctccggaa tgtacgtgtc cagcgcctcc
     3661 atcaactcca ttatcgactt gaactccgca ctgtccagcg gcttgctcaa atccgatccc
     3721 accgtcatcg cctgcaacgc actcccccgg atcaccggca cgtcatcccc gggaaattca
     3781 tactggctca gaagatcccg aacctccaac tccaccaggt ccagcaactc cggatcgtcc
     3841 acctggtcca ccttgttcag gtacaccacg atgtacggaa cccccacctg gcgcgccagc
     3901 aaaatgtgct cccgcgtctg cggcatcggc ccgtccgccg cactcaccac caatatcgcc
     3961 ccgtccatct gcgccgcacc cgtgatcatg ttcttcacgt agtccgcgtg acccggacag
     4021 tccacgtgcg cgtagtgccg cctcgcactc tcgtactcca cgtgcgccgt cgctatcgtg
     4081 atcccccgct cccgctcctc cggcgcattg tcaatgctgt caaacgaacg cacctgcgtc
     4141 cccgtcaccc gcgacaacac catcgtcatc gccgccgtca acgtcgtctt cccatggtcc
     4201 acgtgaccaa tcgtccccac gttcacgtgc ggcttcgtcc gctcaaactt ctgcttcgcc
     4261 ataactccgg ctcgcctccg aaattataca agagcccaga accaggatcg aactggtgac
     4321 ctcgtcctta ccaaggacgt gctctaccaa ctgagctatc tgggcagctc cgatttttgt
     4381 tccacattat ataaactccg ccttccggcc aaaaaaaata ggccacggca gagcccttac
     4441 gggaacccca aatataaaag tgaccctatc gctgtcaagg caaaatcgcc cagccccgca
     4501 aaaaaattgt tgctcccaaa gcgcttagcc ccatccccaa cgaaccctgt ctcgcgctcc
     4561 cggccgcccg cgtctccctc cgccaccctc cttcgctctg cgccggcggc tcgccccctc
     4621 gctccgggtc gccggtcggc aggtagactg acttccctat cccgtccggc cgatccccgc
     4681 cggccagatc ggcaagattc ccacaaaaaa tgctataatg ttgtctcata acaacataag
     4741 cggagtctgt ttgcagtcag agagaataag cgggagacgg gactcgaacc cgcgaccaac
     4801 agcttggaag gctgtgactc ttcctctgag ttactcccgc ctatggggcc tattctagtg
     4861 tggaaacggc cgccgggcaa gctctttctg aactccagtc atgatcccgt gcgtggcggt
     4921 cttccccagt ggagcttgtt gcgcaacacc ttataaaaag aattctccgg aaacacgata
     4981 aatttcagcg tgaactcggc ccgtgtgatc gtcacccgcg aattgtcgaa caactcggtc
     5041 atgacctgcc catcgagcgt cagactcacc acccggtcgg gcgacttcac cgtcacctcg
     5101 agcacatcgt cggcggagaa gagcatcggc cgcgtggtga gcgaaaagga cgagatcggc
     5161 gccaccacaa taccctgcat tttggggtgc aggatcggtc ctcccaccgc caagctgtag
     5221 gccgtcgatc ccgtcggcgt cgcgatcacg attccgtccg cccgatacgt cacaatgtcc
     5281 tccccgttga cgcggaaata cagctcgatc aggcggctca cggcgccctt gtcgaccacg
     5341 atgtcgttga gggcgtaggg ccagctcggc tggtggtccc cggccaccgc ggttttcagg
     5401 agcatgcgct cttcgatcag gtactccccg gccgcgatcc ggtccagggc cgccaccagc
     5461 tgcttgggcg taagctgggt gagaaaaccg agcgacccga gattgattcc gagaatgggc
     5521 gtgccgctgt cgcggatgtc ccgggccgtg gcaagcatcg tcccgtctcc gcccatcgac
     5581 acgatcacgt tggagtatcc ggcgatgtca ctgcgcggca gataggtcag gtgatctccc
     5641 gtgtacccgg ccaggtccgt ggacaggacc acctcgtgcc gcgcctgctg ggcccactcg
     5701 acaaaggtct gaatcgcctc cctcgcccct tggcgcgtca cattggcaaa caggccgagt
     5761 ctcatgcccc gaacataaca ccgcggcgcc gattcaggca acaacttgct gtgaaaaaac
     5821 cccgcccctc actcctcggc ggccccgctg aactcctcct cggtcttccg gcgggcgtgc
     5881 ccgaatcgca tcgcccgtcg caggccgagt ttgcgaagga tcccccggcc gtgctcgtgc
     5941 ccctgctccc gggcgacatt ttccagaagg gtcgcgaccg cttccgcatc gagcccgatc
     6001 tcggccagga gctcctcccg gtccccgtgg gtcacaaacc ggtcggggat accgagggcg
     6061 cggaactggc cgtcccaccc gcgctggagg aggaacgcgc cgaccgcctc gccgaacccg
     6121 ccccgcagtt gtccctcctc catggtgatg atcgcccgcg cgtgatcggc gatctccagc
     6181 agcatggctt cgtcccacgg tttgacgaag cgggcgttga tgatgcccac ccggtcagcg
     6241 ccctcgcgca tcctcgccgc cgcctcctgc gcctgcgcca ccatagtccc gaccgccagc
     6301 acggccacct cgcggcgggg agttacgtac tcccacttcc cccactcgag ggggtcgatt
     6361 tcggggcgca tcgcggtcgg cacggcgccg cgcgggtagc ggatggccac cggccccggc
     6421 aggtcgtggc cggccgtgta gtggagcatc gcccggagct cgttcccgtc tttcggcgcc
     6481 gccaccgcga cattgggcac cgagccgaga tagcacaggt cgaacacgcc gtggtgcgtg
     6541 gggccgtcgt ttcccaccag cccggcgcgg tcgaggcaca tgaccaccgg caggttttgc
     6601 agggcgatgt cgtgcaccac ctgatcgtaa gtgcgctgca ggaaggtcga gtagatcgcg
     6661 aggtacggct tgatgccctc ggcggccagt ccggcggcga aggtcccggc gtgctcctcc
     6721 gcgatcccga catcgaaaaa ccgctcgggg taggcggcgg caaaatcgac cagccccgtc
     6781 ccgtcgcaca tggcggcgct gatcgccgcc acccgccggt ctttggcccc cagttcgacc
     6841 atcgtgtcgc cgaacacctt cgtgaacgtc ggcaggcccg ccgatttcgc cgccgccttg
     6901 cccgtcacct tgtcgaactg cccgaccccg tggaaccgcg agggatctcc ctcggccggg
     6961 cggaaccccc gccccttctg cgtcaccacg tgcaacagga gcggtccgcg gatgttctgg
     7021 agatcgcgca ggcgcttgat gagcagttcg aggtcgtggc cgtcgatcgg cccgaaatag
     7081 cggaagccga gtttttcgaa gagcatgccc ggggtgatca gtccgagcac ggagtcctcg
     7141 atccgcgaaa tggcctcgcg gatccggtcg cgccgcttga accgcccggt cagttcccag
     7201 atttcgttgc gcagccggtt gaacttctcg tccgtcatga tgctcgtgaa gtagcgcgag
     7261 atcgccccca cgttcttcga gatcgaccag gtgttgtcgt tgagcacgac cagcagatcc
     7321 ttcttgagcg atccggcgtt gttgagcccc tcgtaggcca gccctcccgt catcgccccg
     7381 tcgccgatca ccgcgatcac ccgacggtcc tcgcccttct ggtcgcgcgc cgccgcgaac
     7441 ccgagggcgg ctgagatcga agtcgaggca tggccggccc cgaagtgatc atactccgac
     7501 tcctcccgct tggcgaatcc ggccagcccc ttgtactggc ggagggtgtc catgcggtcg
     7561 cgccgcccgg tcagtatctt gtgcgtgtag gtctggttgc tcacgtcgaa aatgaggcgg
     7621 tcgtggggga ggttcagcac gtagtgcagg gccacagtca gttccaccga cccgagattc
     7681 gtcgccagat gcccgcccgt cttcgacacc gaggagatga tcgcctgccg gatctcctcg
//
LOCUS       DAOPOE010000126         7616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10730_length_7616_cov_5.612088, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000126 DAOPOE010000000
VERSION     DAOPOE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10730_length_7616_cov_5.612088"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKM94_12545"
     CDS             <1..684
                     /locus_tag="PKM94_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67503.1"
                     /translation="TLTTRRGETFTGFIAWDVDEVFGQDVLDGEEGDRSRKIQFAKIK
                     TIERYSSSGATVSLVSGSDELVLRGTNDVNDDNRGIIVADPSFGQVIVGWDEFERVEF
                     SRPNEDRASYDAFDGGRPLSGKVATEDGKTYTGRIRWDDDESHTWEILDGDSQDIQFD
                     IEFGAIKRIEKRTNRSSMVTLWDGREFRLRGSNDVDSDNKGIFVTQGDGETVEVAWED
                     FASVEFDRR"
     gene            complement(830..1738)
                     /locus_tag="PKM94_12550"
     CDS             complement(830..1738)
                     /locus_tag="PKM94_12550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67504.1"
                     /translation="MNNRNRIRLLALLGLALVIGLAAAPPAAADAAKAKEAFNAGVKA
                     MQAGNVDEAIVNYKLAIQEDPAYADPAMNLGAIYFDKQAYQEALTAFQTATERDPKRA
                     EAWSNLGKVHYTLKQWVEATTAFQKAADLEPNKVQNFIDLGKTFSQRDMAAEAAASLE
                     KAHQLGGGDYLSWYYLGKAYEGQSKPTEAVAAYEKSIQLKDDFYYSHSALGNYYLSKE
                     KYGLAAGHFKKAAATGDTQAYRAAYNYAIAVQSEDPEDYEAATVAWESFIQSYKNNPK
                     AAKQVAGARETLKALKEAKDQASNMQ"
     gene            complement(1817..2263)
                     /gene="purE"
                     /locus_tag="PKM94_12555"
     CDS             complement(1817..2263)
                     /gene="purE"
                     /locus_tag="PKM94_12555"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOD67505.1"
                     /translation="MPKVLILIGSKSDQEFADTCREQLAALGIDAAIEVSSAHRHPDK
                     TARLAAEAEANGYEVIVAMAGLAAALPGVAAAHSNLPVIGVPLPAALGGLDSLLSIVQ
                     MPPGIPVAAVAIGAPGAKNAAVLAARILALRHPGVRDALRKHRAAL"
     gene            complement(2267..3547)
                     /gene="purD"
                     /locus_tag="PKM94_12560"
     CDS             complement(2267..3547)
                     /gene="purD"
                     /locus_tag="PKM94_12560"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOD67506.1"
                     /translation="MKVLVIGSGGREHALVWKLSKSKKIDKIFCAPGNAGIAQLAKCI
                     SLKPTDTRGLADFALRNKIALTVVGPEDPLAGGLVDEFQKRRLKVFGPDRKGARIEAS
                     KVFAKQFMRKYHIPTAAFRVFSTAAEAIGFCRSVEFPVVIKADGLAAGKGVFVVDNLK
                     AATHALERLMLERSLGKAGDQVIVESCLKGQEVSLMCLTDGKTVVPLLSSQDHKQAFD
                     GDRGPNTGGMGAYSPTTLIDKEMLEQITDFILTPTIKGLQQEGITYRGVLYIGLMITE
                     TGPKVLEFNCRFGDPETQVVLPLLKNDLLDLLLAVTGGKLASVGRLDWRPESAACVVM
                     ASKGYPGSYHAGKRITGLQEIDDSQTFVFHAATTLKGNLLLTAGGRVLNVVAVDRTLS
                     QALARAYRSVHKIRFDGAFFRRDIGARVLKTARR"
     gene            3827..4540
                     /locus_tag="PKM94_12565"
     CDS             3827..4540
                     /locus_tag="PKM94_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67507.1"
                     /translation="MTKTFLSVLAAALLLGSATVCAQEEVVSRDILEISLYGGLAVPV
                     GGIADFATGMGLPLQQADADKLYDRGAKTGWDIAIDLGYYVTPRLTAGVNFRYTEFGI
                     DSDLDGSQAHRLFNPNLYAKYAFEGESYFVPFVKCWVGMENAKFSTAVLNRSAIPGQQ
                     RIFRELSYHPAVAIGASLGLFYYTADYSGLFLEAGYHYAMTDDAVGTYLGADDYVFGE
                     NAGVFTLNAGVKMIIGSGD"
     gene            4617..5966
                     /locus_tag="PKM94_12570"
     CDS             4617..5966
                     /locus_tag="PKM94_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD67508.1"
                     /translation="MADTKLLIVDDEAVQRDLLCGYLAKHGFAVYPCENGAQALDAYH
                     EVFAPVALVDMKMPGMDGITLLGRLREINPFLQVIMLTAFGSVETAVAAMKAGAFDYL
                     TKPVEDLDELLIKLQKAEAQNRLVVDRQAMAQRLAEVFPRTEIIGESPAMQQVRRMIS
                     LVAPKDATVLITGASGTGKELVAQAIHALSGRAEQRLVAINCAAFPETLLESELFGYE
                     KGAFTGADRAKQGRFELADGGTLFLDEIGEMPLSMQVKLLRVLEAKVIQRLGSVKEIP
                     LDIRIVAATNRDLAATVAAGTFREDLYYRLNVITIHVPPLRERPGDILLLADTFVRRF
                     AQKIGRDVKGIEPEAARLLTSYAWPGNVRELQNVLERAVVVCDRDRISPEDLSGLEKA
                     PAAEASEEVLPLSEVERRHIQRCLETRAWNITLCAELLGIHRNTLRAKIKEYGLTQG"
     gene            6188..7045
                     /locus_tag="PKM94_12575"
     CDS             6188..7045
                     /locus_tag="PKM94_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HOD67509.1"
                     /translation="MKKALTFGGLILVLMALAGCEDDDPVYDPIPQPPQGVFSITGDG
                     WVDLWWMYPYDRDIKEFLVYRAEAPMDEPPEEYTLQFRVPAVDNPELNLVWDSTRDNS
                     VDNGKTYWYALATVDHAGRVSDLSAEPVFDTPRPEGVVTLRDYALDVNNAGFDFNGKN
                     VVSAGSPSADVWIDSFDGILYLNAGDTLGTRYTDIQDMGFTGSWDVIGWAPTDGWSNL
                     GWAEIIIGHTYIIWTDDEHFAKLRVISVDGVTGTVMFQWAWQPQVNNPELIAPHGGAT
                     EMTAGRPAM"
     gene            7099..>7616
                     /locus_tag="PKM94_12580"
     CDS             7099..>7616
                     /locus_tag="PKM94_12580"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOD67510.1"
                     /translation="MTRLTVMALAALALAAPAALSQQQSIVRVDRDDTYDYGYEYGDQ
                     AQIDRYLGVEIWPNHHDGEYYEGDRIVLNFRANRDAFVAIYSIDSRGRVNLLFPAGPN
                     EDNFVQGGVTHRLPASQADYDLVVSGPEGVENIQIVASREPFPIPDWYPVSGIMAEGD
                     ERMAYMDYLNDR"
BASE COUNT         1399 a         2195 c         2584 g         1438 t
ORIGIN      
        1 accctgacca cgcggcgggg ggaaacgttc accgggttca tcgcctggga tgtcgacgag
       61 gtcttcgggc aggatgtgct ggacggcgag gaaggggacc ggtcgcggaa gatccagttc
      121 gccaagatca agacgatcga gcgctactcg tcgagcgggg ccaccgtgtc tctcgtctcc
      181 ggcagcgacg agctcgtgct gcgggggacc aacgatgtga acgacgacaa ccgcggaatc
      241 attgtcgccg acccgtcatt cgggcaggtg atcgtgggct gggatgaatt cgagcgggtc
      301 gagttcagcc gtccgaacga ggaccgcgca tcgtatgacg cgtttgacgg cggccggccg
      361 ctctccggca aagtggccac agaagacggc aagacctaca cgggccggat ccggtgggac
      421 gacgacgagt cgcacacctg ggagattctc gacggcgaca gccaggacat ccagttcgac
      481 atcgaattcg gagccataaa acggatcgag aagcgcacca accgatcgtc catggtcacg
      541 ctgtgggacg gccgggagtt ccggctccgg ggctccaacg acgtcgacag cgacaacaag
      601 gggatcttcg taacgcaggg cgacggggag acggtggaag tggcgtggga agattttgcg
      661 tcggtcgagt tcgaccggcg ttagcggcgg caagttcggt gcaatcggaa tgaagggaag
      721 acggcgggtt cgcaggagaa cccgccgttt gcattgcgga ggcacaagaa agcccggtcc
      781 ggggggaccg ggctgttcgc catgagaaac ttctcaccgc acgtgcgggc tattgcatgt
      841 tcgaggcctg atccttcgct tccttcagcg ccttcaacgt ctcgcgggcg ccggcgacct
      901 gcttggcggc cttggggttg ttcttgtagc tctggatgaa ggactcccag gcgacggtgg
      961 ccgcttcgta gtcctccgga tcttccgact gcacggcgat ggcatagttg taggcggcac
     1021 ggtaagcctg ggtatcgccg gtcgcggcgg ccttcttgaa atgcccggcg gccagcccgt
     1081 atttctcctt ggacaggtag taattgccga gggcggagtg cgagtagtag aagtcatcct
     1141 tcagttgaat cgacttctca tacgcggcca ccgcctcggt gggcttgctc tgcccctcgt
     1201 aggccttgcc gagatagtac caggagaggt aatcgccgcc gcccaactgg tgggccttct
     1261 cgaggctggc ggccgcctcg gcggccatgt cgcgctggct gaaagtcttg ccgaggtcga
     1321 tgaagttttg gaccttgttc ggttccaggt cggccgcttt ctggaaggcc gtggtggcct
     1381 cgacccactg cttaagcgtg tagtggacct tgccgaggtt tgaccaggcc tcggcccgtt
     1441 tggggtcgcg ctcggtcgcg gtctggaagg cggtgagggc ttcctggtag gcctgcttgt
     1501 cgaaatagat ggcgccgagg ttcatcgccg ggtcggcgta agccggatct tcctgaatag
     1561 ccagtttgta attgacgatc gcctcgtcga cattgcccgc ctgcatggcc ttgacgccgg
     1621 cgttgaacgc ttctttggct ttggcggcgt cggccgcggc ggggggtgcg gcggcgagcc
     1681 cgatcacgag cgctaggccg aggagggcga gcagacgaat tcgattacgg ttgttcatag
     1741 gtctcccttc agtcttccac tatccaatgt ccaatttgtt atttgtacca gttcccagcc
     1801 gattcgttca gggttttcac agcgcggcgc ggtgcttgcg cagagcatcg cggacacccg
     1861 ggtgccggag cgcaagaatg cgggcggcca gcacggcggc gtttttcgcc ccgggggcgc
     1921 cgatggcgac ggcggccacc ggaatgccgg gcggcatctg cacgatcgag agaagtgagt
     1981 ccagtccgcc gagcgccgcg ggcagcggca cgccgatgac cggaaggttg gagtgggcgg
     2041 cagctacgcc cggcagagcg gccgcgagac cagccatggc gacgatcacc tcgtacccgt
     2101 tcgcctcggc ttccgccgcc agacgggcag tcttgtccgg atgccgatgg gcggacgaga
     2161 cctcgatggc ggcgtcgatg ccgagcgcgg cgagctgttc ccggcacgtg tcggcgaatt
     2221 cctggtcgga tttcgaacca atgaggatga gtactttggg catgcatcat ctccgcgcag
     2281 ttttgagcac tcgagccccg atatcgcgcc gaaagaaagc gccgtcgaaa cgaatcttgt
     2341 gcaccgaccg gtaggcccgg gcgagcgcct ggctaagcgt cctgtcaacc gccacgacat
     2401 tcagcacccg cccgccggcc gtcagcagta ggttcccttt tagggttgtc gcggcatgga
     2461 aaacgaacgt ctgactgtcg tcgatctcct gcagaccggt gatgcgtttg ccggcgtggt
     2521 agctccccgg gtagcccttc gaggccatga cgacgcaggc ggcggattcc gggcgccagt
     2581 caagccgccc gaccgaggcc agcttgcctc cggtaacagc gagaagaaga tccagcagat
     2641 cgttttttaa cagcggcagg acaacttggg tttcggggtc gccgaatcgg cagttgaatt
     2701 caagcacttt tggtccggtt tcggtgatca tgagaccgat atacagcacg ccgcggtagg
     2761 tgatcccttc ctgctggagg cccttgatcg tgggagtgag gatgaagtcg gtgatctgct
     2821 ccagcatctc tttgtcgatc aatgtggtag ggctgtatgc gcccatcccg ccggtattgg
     2881 gcccgcggtc tccgtcaaat gcctgcttgt ggtcctgact ggacagaagg gggacgaccg
     2941 tcttcccatc ggtaaggcac atgagcgaca cttcctgacc tttcaggcag gactcgacaa
     3001 tgacctggtc gccggccttg cccagagaac gctccagcat gagtctctcc agagcgtggg
     3061 tggcggcctt gaggttgtcg acgacgaaga cgcccttgcc ggccgccaag ccgtcggcct
     3121 tgatgaccac cgggaactcc acgctgcggc agaagccgat ggcttcggcg gccgtggaga
     3181 acacgcggaa ggcagcggtg ggaatgtggt atttgcgcat gaactgcttg gcaaacacct
     3241 tgctggcttc gatgcgggcg cctttgcggt cggggccgaa gactttgagg cggcgcttct
     3301 ggaattcgtc gaccagcccg ccggcgaggg gatcttccgg tccgaccacg gtgagggcga
     3361 ttttgttgcg gagggcgaag tcggcgaggc cgcgggtatc ggtgggtttg aggctgatac
     3421 acttggccaa ctgggcaatc ccggcgttgc cgggcgcgca gaagatcttg tcgattttct
     3481 tggacttgct cagcttccag acaagagcat gttcccgccc gccggaacca atcaccagaa
     3541 ccttcatagc tctccttcga gcatgtcgtc aaataaacca gaacctgaat tataggagaa
     3601 tatcccccga attcaagcgc ttttttctcc cgacgcgcgg tcgcgggaac gggggcgcgg
     3661 cccgcgccgc agaatgcggg ggcgccacat aatctgttga caaaggatag ccccgaatgt
     3721 ttattgcccg aaattacggc ggccggtctt tgtcttgaac agcgaccggc gcgcgaaccg
     3781 tctttgtttg actaggagtt cgtttacaca tattcgggga ttgcgaatga caaagacatt
     3841 tctatcggtg ttggccgcgg ccctgctgct gggcagcgcg acggtgtgcg cgcaggagga
     3901 ggtcgtctcc cgggacattc tggagatcag tctgtacggc ggcttggccg ttccggtcgg
     3961 cggtatcgcg gatttcgcca ccgggatggg gctgccgctt cagcaggcgg atgccgacaa
     4021 gctgtacgac cgcggagcca aaaccggctg ggacatcgcc attgatctcg ggtactacgt
     4081 gacgccgcgg ttgacggccg gcgtcaactt ccggtacacg gagttcggga ttgactcgga
     4141 cctcgacggg tcgcaggcgc accggctgtt caatccgaac ctgtacgcca agtatgcgtt
     4201 tgagggcgag tcctattttg tgcccttcgt gaaatgctgg gtgggaatgg agaacgcgaa
     4261 attcagcacc gccgttctga accggagcgc catcccgggc cagcagcgga tcttccgcga
     4321 actatcgtat catcccgcgg tggcgatcgg cgcctcgctc ggcctcttct actacacggc
     4381 cgactatagc ggcctgttcc tggaagccgg gtaccactac gcgatgaccg acgacgccgt
     4441 ggggacctac ctgggggcgg acgactacgt gttcggtgag aacgcgggcg ttttcaccct
     4501 caacgccggc gtgaagatga tcatcggttc cggggactga tgcgaactta agcacgacaa
     4561 gcccgtcggt caccggcggg cttttttatt ccggccgcgg gaggcccggg gcaaacatgg
     4621 cggacacaaa actcctcatt gtcgacgacg aggcggtgca gcgcgacctg ctctgtggat
     4681 atctggccaa gcacgggttc gccgtctatc cctgcgagaa cggcgcgcag gcgctcgatg
     4741 cgtaccacga ggtgttcgcg ccggtggccc tggtggacat gaagatgccg ggcatggacg
     4801 ggatcacgct gctgggcagg ttgcgcgaga tcaacccgtt tctccaggtg atcatgctga
     4861 cggcgttcgg gtcggtagag acggcggtgg cggcgatgaa ggccggggcc ttcgactacc
     4921 tcaccaaacc ggtcgaggat ctcgatgaac tgctgatcaa gctgcagaag gcggaggcgc
     4981 agaaccgcct ggtggtcgac cgccaggcca tggcgcagcg gctggcggag gtgtttcccc
     5041 ggaccgagat tatcggggaa tcgccggcca tgcagcaggt ccggcgcatg atctcgctgg
     5101 tggcgccgaa agacgccacc gtgctcatca cgggggcttc gggaaccggc aaggagctgg
     5161 tggcccaggc gatccacgcc ctctcggggc gggccgagca gcggctcgtg gcgatcaact
     5221 gcgccgcctt cccggagacg ctgctggaat cggagctctt cgggtacgag aagggagcgt
     5281 tcaccggcgc cgaccgggca aagcagggac ggttcgagct ggccgacggc ggaaccctct
     5341 tcctcgacga aatcggggag atgccgctgt cgatgcaggt gaaactgctc cgggtgctgg
     5401 aggccaaggt gatccagcgg ctcgggtcgg tgaaagaaat tccgctggat atccgcatcg
     5461 tggcggccac caaccgcgac ctcgccgcca cggtggccgc cgggacgttt cgcgaggatt
     5521 tgtactatcg gctcaacgtg atcaccattc acgtgccgcc gctccgcgag cggccgggag
     5581 atatccttct actcgccgac accttcgtgc ggcggtttgc gcagaagatc gggcgggacg
     5641 tgaaggggat cgagccggag gcggcgcgcc tgctcacctc gtacgcgtgg cccgggaatg
     5701 tccgcgagct gcagaacgtg ctggagcggg ccgtggttgt ctgcgaccgc gaccgcatct
     5761 cgccggagga cctctcgggg ctggaaaagg cgcccgcggc cgaggcctcg gaagaagtgc
     5821 tgccgctgtc ggaggtggag cggcgccaca tccagcggtg cctggagacg cgcgcgtgga
     5881 atatcaccct gtgcgcggaa ctcctgggaa tccaccggaa tacgctgcgg gcgaaaataa
     5941 aggaatacgg gctcacgcag ggctaggcgc caaccgtcaa atctaccgct tcccggctgc
     6001 agcgactccc cgacaatttc tgtgcttccc ggcgcgcgac cgcacaattt ttgtgcatcg
     6061 cggaccgctc cggattccct gttatgtgta taagaaattg attagcaatg agatgcaccg
     6121 attggcacgg cttttgatga atagtcgtgt gaaggtaacg aaaaccagta tggcaggagt
     6181 ttgggatatg aagaaggcgt tgacattcgg cggtctgatt ctggtactga tggccctggc
     6241 cggctgcgag gatgacgatc cggtgtacga tccgattccg cagcccccgc agggagtgtt
     6301 ctccattacc ggcgacggat gggttgatct ctggtggatg tatccctacg atcgggatat
     6361 caaggagttc ctcgtctacc gggctgaggc gccgatggac gaaccgcccg aagagtacac
     6421 gctgcagttt cgcgtgccgg ccgtggataa cccggaactg aacctggtct gggactcgac
     6481 gcgggacaac tcggtggaca acgggaaaac gtactggtat gcgctggcga ccgtggacca
     6541 cgcgggccgg gtttcggatt tgtcggccga gccggttttt gacacgcccc ggccggaggg
     6601 cgtggtgacg ctgcgggact acgcgctcga tgtaaacaac gccggttttg acttcaacgg
     6661 caagaacgtg gtgtcggccg gaagcccctc ggccgacgtc tggattgact cttttgacgg
     6721 tatcctctat ctgaatgcgg gcgacaccct cggcacgcgc tacacggaca ttcaggacat
     6781 ggggttcacg gggagttggg atgtgatcgg ctgggcgccg accgacggct ggtcgaacct
     6841 cggctgggcg gagatcatca ttggacacac ctacatcatc tggacggatg acgagcattt
     6901 cgccaagctg cgggtgatca gcgtggatgg ggtcacgggg acggtgatgt tccagtgggc
     6961 atggcagccg caggtgaaca acccggagtt gattgcgccc cacggcggag cgacggagat
     7021 gacggcggga cgtcccgcca tgtaatagac tgagtgagaa cacgaatcgg aaagaggtga
     7081 tagacgatgt tgcggcaggt gaccagactg acagtgatgg cgctcgcggc gctggccctg
     7141 gcggcgccgg cagcgctgtc gcagcagcag agcatcgtac gcgtcgaccg cgacgatacc
     7201 tacgactatg ggtatgagta cggggaccag gcgcagatcg accgctacct cggggtcgag
     7261 atctggccga accatcacga cggcgagtac tatgaggggg atcggatcgt gctgaatttc
     7321 cgggccaacc gggacgcctt tgtggcgatc tacagcatcg actcgcgggg acgggtgaac
     7381 ctcctgttcc cggccgggcc gaacgaggac aacttcgtcc aagggggggt gacacaccgg
     7441 ctgcccgcgt cccaggccga ctatgacctc gtggtctcgg gtcccgaggg agtcgagaat
     7501 atccagattg tcgcgtcgcg cgagccgttc cctatccccg actggtaccc ggtgtcgggc
     7561 atcatggcgg aaggggacga acgcatggcg tacatggatt atctgaatga ccggta
//
LOCUS       DAOPOE010000127         7587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10778_length_7587_cov_5.512746, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000127 DAOPOE010000000
VERSION     DAOPOE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10778_length_7587_cov_5.512746"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKM94_12585"
     CDS             complement(<1..362)
                     /locus_tag="PKM94_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HOD67511.1"
                     /translation="MTRTLYLAAAGCAVALLLSAAGAAAGEANSRAGTSAFSFLKIDV
                     GARAVAMGGAGTGLADDESALYYNPAGIVGFEQRRFVGGYHSYFADMQSGFVGYIAPI
                     GLTKAVGAYLSYLNYGDFV"
     gene            complement(595..1092)
                     /locus_tag="PKM94_12590"
     CDS             complement(595..1092)
                     /locus_tag="PKM94_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOD67512.1"
                     /translation="MHRQAYRSVAAALFLTGLGLSCGVYTFNPAGKSSIKTIAVSRFE
                     NQTVELALTDQITDGVIDAFIADGTLAVAPEDAADAVLAGTLVSYTRAPYQYTSGDAV
                     QSYRVTMDFDIALVNPKDNSEIWKERMSQIGIYDPATETEEDAQRKAIQNLVQAILNR
                     TTKSW"
     gene            complement(1119..2327)
                     /gene="hutI"
                     /locus_tag="PKM94_12595"
     CDS             complement(1119..2327)
                     /gene="hutI"
                     /locus_tag="PKM94_12595"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOD67513.1"
                     /translation="MAGPVPRLGPALRDIGLIAHGAIAAAGDEIVAVGPSDDIERHTR
                     LAENCTVIDAKGAVVTPGLIDPHTHPVFSMTREKEFEMRIEGKTYMEIAAAGGGIRAS
                     VRDLRSADRAVLLEQTRRRVARMLRYGMTTIEAKSGYGLSTESELRQLEIIREVDQQC
                     PIDLIPTFLGAHEVPDEYRERRSEYVDLLVGEMIPAVAAAKLAEFSDIFTEVGVFEIE
                     DSRRIQQAAKEAGLKLKFHADEIKPLGGAELAAAMGAVSADHLVHVSERGIKALAAAG
                     TVAVLLPGTSFSLASSYAPARKMIEGGVTVALSTDCNPGSSCTESLPFIVSLAALQLK
                     MTAAEALAAVTVNAACAVDRAATLGRLVPGLRADLVIWDMNDYRELPYHYAVNLVSRV
                     IKNGKVVVSQ"
     gene            2595..4559
                     /locus_tag="PKM94_12600"
     CDS             2595..4559
                     /locus_tag="PKM94_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOD67514.1"
                     /translation="MPASDSTGLPVIRTYLSALVVFFLLRLGFLAWHAALFRGAAGTE
                     LARAFLGGAVIDSSVTACTLLAAWLIALAALAAGRTAARRVFLGLCAILFGLYFFINL
                     ADIAYFDMFDSRLNVLVFENLDQMAPILQTIASGWSVVIVTIVWLAVLTAFILALRAR
                     SRVGGYSRRGARRVISGITVGLLLAALTFLWVGEPFWRLGLFSSGNQALTQLSLNGVY
                     TLLKAYHQKRIWERDFGDIDYAFGPPEEALQRVRREIIGDDEAASEGRFPFARELRRP
                     APLAVAKPNIVIILMEGFTASGVGALCPGGIGDSPGFDRLAHEGILFTNFYGAGTRTH
                     HGLVSTVASFPSLLELFLTRRRGTESFSTLGTLLRAYGYRTSFFYGYDAGFDHMGFFM
                     RQGGFDLIVDQRDFAAPRFVGKWGVSDEDLFAKVSAYLSGQAEGEPVLSVVMTSSNHT
                     PHDIPPEFAAARPQYAGDRARAAFAYSDYALARFLDGERDKPYFRRTIFVIVADHGEI
                     RDRADRYFKRFQIPCLLYAPGLIPGGRTVSTVGSQADIATTLMHLIGYPRPFHFLGRD
                     LLALPEDRGFAVMRNNSVLYYRKGGRVLVRDIRDTASVLYPLDSEGRMMQERPYRDDS
                     LQQALNRDLEAYLQSLYLLFTTGRHRCPES"
     gene            complement(4601..6262)
                     /gene="hutU"
                     /locus_tag="PKM94_12605"
     CDS             complement(4601..6262)
                     /gene="hutU"
                     /locus_tag="PKM94_12605"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOD67515.1"
                     /translation="MPDKTIRAPRGNRLHCKGWHQEAAFRMIQNNLDPEVAEKPGELI
                     VYGGMGKAARNWDSFRAILKSLRTLENDETLLVQSGKPVGIFRTHDYAPRVLLANSNL
                     VPAWATWDYFRKLDALGLIMYGQMTAGSWIYIGSQGIIQGTYETFMAAARKHFGGDLA
                     GRWILTGGMGGMGGAQPLAATMAGATILVVEVDETRIDRRVKQGFCDVKVRTLDEALK
                     LIETHTQAGAQPISIGLVANAATVFSQLHRLGVYPDMITDQTSAHDELNGYIPEGLTP
                     AEAAALRRKNPADYIKRSYRSMVKHCEAMVRYHEDGAIVFDYGNNLRGQAEKGGLKNA
                     FSYPGFVPAYIRPLFCEGKGPFRWAALSGKPADIDVTDRIVKSNFSSNAPLMRWIDLA
                     RKRIPFQGLPARICWLGYGERARFGEVLNDYIRKGKISAPVVIGRDHLDTGSVASPYR
                     ETEGMKDGSDAIADWPLLNALLNTASGASWVSIHHGGGVGMGNAIHAGQVIVADGTKM
                     MAARLQRVLTNDPGSGIIRHADAGYAEAIKAARAHKIKIPMRTRK"
     gene            6425..7333
                     /locus_tag="PKM94_12610"
     CDS             6425..7333
                     /locus_tag="PKM94_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD67516.1"
                     /translation="MTSRNGPEIESLFSNPGDHRRLHYRGGLDKIGRWTDGLLVTEDG
                     RVSFPVEDGIPRFHLGDGNGWDDEALREAEFRRRGVKPDRLIERNYDYMVNQWPDHEV
                     YLPWLRSIAASRGPIVELAVGPGGGAAMVVLDLNPHAHYLMNDIGLWPLRQWQQFART
                     AARWPNLRFAQFDAAEMPFASRTVAAVVSMAGISNIGRQFEAIREAFRVLRPGGWFYA
                     VEVLPDHKLTDKCPPEMVAKLKELFPAMERGFREVLAGIGFAPVRIRPMGKRPLVPGE
                     GGLADFCKRHEVVIHLRFLRIRARKR"
BASE COUNT         1223 a         2371 c         2613 g         1380 t
ORIGIN      
        1 acgaagtcgc cgtagttgag gtacgacagg taggcgccga ccgccttggt caggccgatc
       61 ggggcgatgt agccgacgaa cccggactgc atgtcggcga agtagctgtg gtagcccccg
      121 acaaaacggc gctgttcgaa gccgacgatg ccggccggat tgtagtagag ggccgattca
      181 tcatcggcca gcccggtgcc ggcgccgccc atggcgaccg cccgggcgcc cacgtcgatt
      241 ttcagaaacg agaaggcgct cgtgccggct cgcgaattgg cctcaccggc agccgcgccg
      301 gcggccgaca gaagcagcgc gaccgcacaa cctgcggccg cgaggtacag ggttcgcgtc
      361 attggtgcca tccttccaga gcgatgatac ggttccgcgc cgcggctgtc aacagcaata
      421 gggcgcgccc ggccaaaggc cggcggacgc ccggggcgat gcggcgccgg cgggccccgg
      481 gagggtcccc cgggagcagc ccggcgcaaa cgggaggagc gggcctacgc ccggtccggt
      541 tcttatactc cccagccgcg gaaagaatcc ggcgctccgc cgccgatcac gccctcacca
      601 gctcttcgtg gtgcggttga ggattgcctg gacgaggttc tgaatcgcct tccgctgtgc
      661 atcctcctcc gtctcggtgg cgggatcgta gatgccgatc tggctcatgc gctctttcca
      721 gatctcggaa ttgtccttcg gattgacgag ggcgatgtcg aaatccatgg tcacgcggta
      781 cgactgcacg gcgtcgccgg aggtgtactg atagggggcg cgcgtgtagc tcaccagcgt
      841 gccggcgagc acggcgtcgg cggcatcctc gggcgccacc gccagcgtgc cgtcggcaat
      901 gaaggcgtcg atgacgccat cggtgatctg atcggtcagc gcgagctcga ccgtctggtt
      961 ttcaaaccgg gagacggcga tggttttgat cgaagatttc cccgccgggt taaatgtgta
     1021 cacgccgcag gaaagcccca agcctgtgag aaacagggcc gcggcaaccg accgatacgc
     1081 ctggcgatgc atgatcgttc tctcctcgcg cgggccgctc attggctcac gaccactttt
     1141 ccgttcttga tcacccggga gacgagattg accgcatagt ggtaaggaag ctcccggtag
     1201 tcgttcatgt cccagatcac cagatcggcg cgcaggccag ggacgaggcg gccgagagtc
     1261 gcggcccggt cgacggcgca ggcggcgttg accgtcaccg cggccagcgc ctcggcggcc
     1321 gtcatcttca actggagcgc cgccagcgac acgatgaagg ggagcgactc ggtgcagctt
     1381 gaacccgggt tgcagtcggt cgagagtgcg accgtcacgc cgccctcgat catcttccgc
     1441 gccggggcat aggacgaggc gagggagaag gaagttccgg gcagcaggac cgccaccgtg
     1501 ccggcggcgg ccagggcctt gatcccccgt tcggatacat ggacgaggtg gtcggccgag
     1561 accgcgccca tggcggcggc cagctcggcg ccgcccagcg gcttgatctc atcggcgtgg
     1621 aatttcagct tcagcccggc ctccttggcg gcctgctgaa tgcggcgcga atcctcgatc
     1681 tcgaacacgc cgacctcggt gaagatgtcg gagaattcgg ccagtttggc cgcggccacc
     1741 gccgggatca tttcgccgac gagcaggtcc acatactcgc tccgacgctc gcggtattca
     1801 tcggggactt cgtgcgcgcc gaggaacgtc ggtatcagat cgatcgggca ctgctggtcg
     1861 acctcgcgga taatctcgag ctggcggagc tcggactcgg tcgaaaggcc gtacccggac
     1921 ttggcctcaa tcgttgtcat gccgtagcgg agcatccggg cgacacgcct ccgcgtctgt
     1981 tcgagcagga ccgcgcggtc ggcggagcgg aggtcgcgga cggaggcgcg gatgccgccg
     2041 ccggccgcgg cgatctccat gtaggtcttc ccctcgatgc gcatttcgaa ctccttctcg
     2101 cgcgtcatgg agaagaccgg gtgggtgtgg ggatcgatga ggccgggggt gacgaccgcc
     2161 cctttggcgt cgatgacggt gcagttttcg gcgagccggg tgtggcgttc gatgtcgtcg
     2221 gatgggccga cggcgacgat ctcatcgccg gcggcggcga tcgcgccgtg cgcgatcagc
     2281 ccgatgtcgc ggagagcggg gccgaggcgg gggaccgggc cggccatcgt gatcacctgg
     2341 ccggcgtttt tgaccaaaag ggtggctttc ttgttcggcg gcaacgtgcg tctgtccttc
     2401 ggtttctcgt cgtccggttc ggtctacggc ccgaatatac acaaccgccg cgcccgcgct
     2461 aatacaattt gacaagcggg gtcggcgcgc ggcatactgc gcccgggtcg gggctcggcg
     2521 tccggagaaa ccgggcgggg cgcgacccgc cgcgcggccg caccgcgtcg ccgaccattc
     2581 acaggcaggg taccatgccg gcgtccgaca gcaccggtct tccggtcatc cgcacttacc
     2641 tgagcgcgct tgtggtgttc ttcctgctgc gcctcgggtt tctcgcctgg cacgcggcgc
     2701 ttttccgcgg cgcggcgggg acggaactgg cgcgggcctt tctcggcggg gcggtgatcg
     2761 attcctcggt gaccgcgtgc accctcctgg cggcgtggct gatcgcgctg gcggcgctcg
     2821 ccgcgggccg caccgccgcg cggcgcgtct tcctcggcct gtgcgcgatt ctgttcggcc
     2881 tctacttctt catcaatctg gccgatatcg cctactttga catgttcgac tcccgcctga
     2941 acgtcctcgt tttcgaaaac ctggaccaga tggcgccgat tctgcagacg atcgcctccg
     3001 ggtggtcggt cgtgattgtc acgatcgtgt ggctggcggt cctgaccgct ttcatcctgg
     3061 cgctgcgcgc ccgctctcgg gtcggcgggt attcccgccg aggggcgcgc cgggtcatct
     3121 cggggatcac cgtcggactg cttctcgccg ccctcacctt cctgtgggtg ggcgaaccct
     3181 tctggcggct cggcttattc tcctccggca accaggcgct cacccagctc tcgctgaacg
     3241 gcgtgtacac gctcctcaaa gcctaccatc agaagcggat ctgggagcgc gacttcgggg
     3301 acatcgacta tgccttcggg cctccggagg aggcgctgca gagggtgcgt cgggagatta
     3361 tcggcgacga tgaggcggcg agcgagggaa gatttccgtt cgcccgggaa cttcgccggc
     3421 ccgcgcccct cgccgtggcc aaacccaaca tcgtgatcat tctcatggaa gggttcaccg
     3481 ccagcggggt gggagcgctc tgtcccggcg ggatcggcga ctcgcccggg ttcgaccgcc
     3541 tggcgcacga gggaattctc tttacgaact tctatggcgc cggcaccagg acgcaccacg
     3601 gtctggtcag cacggtggcc tcgttcccct cgctcttgga gttgttcctc acgcggcggc
     3661 gggggacgga gtcgttctcg acgctgggga cgctgctgcg cgcctacgga taccggacga
     3721 gtttcttcta cggctacgat gccgggttcg accacatggg tttcttcatg cggcagggag
     3781 ggttcgatct gatcgtcgat caaagggact tcgccgcgcc ccgcttcgtc ggcaagtggg
     3841 gcgtctccga tgaggacctg ttcgccaaag ttagtgcgta cctgtccggc caggccgagg
     3901 gggagccggt gctgagcgtg gttatgacgt cgtccaacca cacgccgcac gatattcccc
     3961 cggagttcgc ggccgcgcgg ccgcagtatg ccggagaccg ggcccgggcc gccttcgcct
     4021 actccgacta cgccctcgcg cggtttttgg acggagaacg tgacaagccc tattttcgcc
     4081 gcacgatctt cgtgatcgtc gccgatcacg gggagattcg ggaccgagcc gaccgctatt
     4141 tcaagcgctt tcagattccc tgcctgctct acgcgccggg gttgattccg ggaggccgga
     4201 ccgtgtcgac cgtgggcagc caggcggaca tcgccacgac gctcatgcac ctgatcggct
     4261 acccccggcc cttccacttc ctcggccgcg atctcctggc gcttcccgaa gaccggggat
     4321 tcgccgtcat gcggaacaac agcgtgctgt attaccgcaa aggcggccgc gttctggtcc
     4381 gggacatccg cgacacggcc tccgtcctct atcctctgga ttctgaaggg cggatgatgc
     4441 aggaacggcc ataccgcgac gacagtctgc aacaggcgct gaaccgcgac ctggaggcct
     4501 acctgcagag tctgtacctg ctgttcacga cggggcggca ccgctgcccc gaatcgtgag
     4561 cgcggcgccg tccgggcgga ccggcgcccc gtccccgcgg ctatttccgc gtccgcatgg
     4621 ggatcttgat cttgtgcgcc cgcgccgctt tgatcgcctc cgcgtagccg gcgtcggcgt
     4681 ggcggatgat gccgctgccg gggtcgttgg tgaggacacg ctggaggcgt gcggccatca
     4741 ttttggtccc gtcggcgaca atgacctgac cggcgtggat ggcgttgccc atccccacgc
     4801 cgccgccgtg gtggattgac acccacgagg cgccggaggc ggtgttgagc agggcgttca
     4861 gcagcggcca gtcggcgatc gcgtcggaac cgtccttcat cccctcggtc tcgcggtagg
     4921 gcgaagcgac cgagccggtg tcgagatggt cgcggccgat cacgaccgga gccgaaatct
     4981 tccccttcct gatgtaatcg ttgagcacct cgccgaagcg ggcgcgctcg ccgtagccga
     5041 gccagcagat gcgcgccggg agaccctgga acgggatacg ctttcgggcc aggtcgatcc
     5101 agcgcatgag cggcgcgttg gagctgaaat tcgacttcac aatccggtcg gtgacgtcga
     5161 tatccgcggg cttgccggag agcgccgccc agcggaacgg acccttgccc tcgcaaaaca
     5221 gcgggcggat ataggccggg acaaatccgg ggtaggagaa agcgtttttc agccccccct
     5281 tctccgcctg gccgcggagg ttgttgccgt agtcaaagac aatcgcgcca tcctcgtggt
     5341 agcggaccat ggcctcgcag tgcttgacca tcgaccggta ggaccgcttg atgtagtcgg
     5401 ccgggttctt ccggcgcagc gcggccgcct cggccggggt cagaccctcg gggatgtagc
     5461 cgttgagctc gtcgtgggcc gaggtctgat cggtaatcat atcggggtag acgcccaggc
     5521 ggtggagctg cgagaagacg gtggcggcgt tggcgaccag gccgatcgaa atgggctgcg
     5581 cccccgcctg agtgtgcgtc tcgatcagct tgagcgcctc gtcgagcgtg cgcactttga
     5641 cgtcgcagaa accctgcttg acccggcggt cgatgcgggt ctcgtcgacc tcgaccacga
     5701 ggattgtggc gccggccatg gtcgcggcca gcggctgggc cccgcccatc ccccccatcc
     5761 cgccggtgag aatccaccgc ccggcgaggt cgccgccgaa gtgcttccgg gcggcggcca
     5821 tgaacgtctc gtaggttccc tgaatgatcc cctggctgcc gatgtagatc cagctgccgg
     5881 cggtcatctg gccgtacatg atgagaccga gggcgtcgag tttgcgaaaa tagtcccagg
     5941 tggcccaggc ggggacgagg ttcgagttgg ccagcagcac gcggggagcg tagtcgtgag
     6001 tgcggaagat acccaccggt ttgccggact gcacgagcag ggtctcgtcg ttctcgagcg
     6061 tccgcagcga cttcagaatg gcgcggaatg agtcccagtt gcgggcggct ttgcccatcc
     6121 cgccgtacac gatcagttcc cccggtttct cggcgacctc ggggtcgagg ttgttctgga
     6181 tcatgcggaa ggcggcttcc tggtgccagc ctttgcagtg cagccggttg cctcgggggg
     6241 cgcggatggt tttatcgggc atggtcgatc tccttgggtc aggccgtcat gatacaccaa
     6301 tataggacac ggcgaagcag ggggcaaggg gggcggccac ggatatggat tgtgcgcgcg
     6361 gtgatcgcat agtacattga gaacagcgcc ggcggcggcg ccgccggcaa ccggggtaat
     6421 gcacatgaca agtcgaaacg gcccggagat cgagtcgctc ttctcaaacc cgggggacca
     6481 tcgccggttg cactaccggg gaggccttga caaaatcggt cgctggaccg acggcctgct
     6541 ggtcaccgag gacgggaggg tctcgttccc ggtcgaggat ggaatcccgc ggtttcatct
     6601 tggcgatgga aacgggtggg atgacgaggc gctccgggag gccgagttcc ggcggcgcgg
     6661 cgtcaaaccc gaccggttga tcgagcgcaa ctacgactac atggtgaacc agtggccgga
     6721 ccacgaggtc tacctcccct ggctgaggtc gatcgcggcc agccgggggc cgattgtgga
     6781 gctggcggtg ggacccggag ggggggcggc gatggtggtg ctcgacctga atccgcacgc
     6841 gcattacctg atgaacgata tcggcctgtg gcccctgcgg cagtggcagc agttcgcgcg
     6901 caccgcggcg cgctggccca acctgcggtt tgcgcagttt gacgccgccg agatgccgtt
     6961 cgcctcgcga acggtcgcgg cggtggtgag catggcggga atctcgaata tcggccgcca
     7021 gttcgaggcg atccgcgagg ccttccgggt gttgcggcca gggggatggt tctatgcggt
     7081 ggaggtgctc cccgaccata agctcaccga taagtgcccg cccgagatgg tggcgaagtt
     7141 gaaggagctg ttcccggcga tggagcgcgg gtttcgggag gtgctggccg ggatcgggtt
     7201 tgccccggtg cggattcgcc cgatgggcaa gcggccgctg gtgccgggcg agggggggct
     7261 ggcggacttc tgcaaacgac acgaggtagt tatccatttg cgattcctga ggatacgggc
     7321 aagaaagcgg tagggggacg gggccttggg aagtatccag cgagggccgg tggaaaaaaa
     7381 cactcggcca ggtctcttat ggcgcgtcca atcagtcata aaaatgtaga aaaagtggtg
     7441 acggtggatt aggaaaaacc gaggcgtcgg aaacggaaaa tattaagtac aattattata
     7501 gctacttaag cgctcatatc ctaatcagca aaaattatgc tggattaaac gtttgagttt
     7561 ggtatatttg attatggtca cgctcga
//
LOCUS       DAOPOE010000128         7581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10787_length_7581_cov_5.256444, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000128 DAOPOE010000000
VERSION     DAOPOE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10787_length_7581_cov_5.256444"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PKM94_12615"
     CDS             <1..1147
                     /locus_tag="PKM94_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOD67517.1"
                     /translation="GLPVTPGARLDGAGGEEALAAARQIGFPILVKAAAGGGGKGMRI
                     VAAPGEFAEALESASREAKSAFGDGRVYIEKYLARPRHVEIQILCDRHGNAVHLFERE
                     CSIQRRHQKVIEESPSPMMTPELRARMGDAAVRLARESGYVGAGTVEFLVDRDRSFYF
                     LEVNTRLQVEHPVTEMVTGIDLVAEQIAVAEGERLRWRQEDIRLHGHALECRIYAEDP
                     EEGFLPSTGTLRNYRAPSGPGVRVDSGVVVYSEIPVYYDPIIAKLIVWGRDRDEAIRR
                     AGRALVEYRIGGVQSTIEFHRAVLRNPKFLAGDLSTRFLEEEFPENNYLVLDDKLREQ
                     IAIAAALETAARERTIIVQNNARGGAAPSNWVLVHRRSVLKPFSGSR"
     gene            1147..1659
                     /locus_tag="PKM94_12620"
     CDS             1147..1659
                     /locus_tag="PKM94_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67518.1"
                     /translation="MRRYLITVDGREFDISLDPSMQSGRLLCNGRPREIITHLLGPSR
                     ALVLIDNESLEVDVRANAYDTKKTLFVHGMEIPVTIEDYHLAQLRKTAGIRSGAEIDR
                     VLRAAMPGLVLEVKVRPGEIVRRSQPLLIIEAMKMENIIKAQGEGTVKTVFVAPGKSV
                     EKNDKLLEFA"
     gene            1659..3233
                     /locus_tag="PKM94_12625"
     CDS             1659..3233
                     /locus_tag="PKM94_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD67519.1"
                     /translation="MSDDTKLTTSGIPIPVTATAADAPAAPGPRHAGEYPFTRGVYPT
                     MYRGRLWTMRQYAGFGTAAETNRRFKYLLQRGQTGLSVAFDLATQIGYDSDHPMAAGE
                     VGRTGVAIDSLADMEKLFDGIPLDKVSTSMTINAPAVILLAMYIAVGRKQGVPAAQLS
                     GTVQNDVLKEFIARGTYILPPKPSMKIITDIFAYAAKHLPKYNTISISGYHIREAGST
                     AAQEVAFTLANAVAYVEAALAAGLSIDDFAPRLSFFFAAHNDLFEEVAKFRAARQIWA
                     ELVRDRFRSRDEKSMLLRFHTQTGGSTLTAQQPENNIVRTAIQALAGVLGGTQSLHTN
                     SYDEALGLPTEKSVEIALRTQQIIAHESGAAATVDPLGGSYYIEYLTAAMDKRIRALL
                     AEIESRGGAVACIENGYFRDEIAAAAYDFQRRVETRRQIVVGVNEFTSHSPGIPAVLR
                     VDPELERNQVRELAKLKATRDRKAVAESLAAVRDAARQNKNVVEPIIDAVAHYATVGE
                     ISDIFREVWGEYHENL"
     gene            3220..3741
                     /locus_tag="PKM94_12630"
     CDS             3220..3741
                     /locus_tag="PKM94_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67520.1"
                     /translation="MKTFKLRRLAFAGALLALVGCGGQSDTDTADSAAAAGADSLTLD
                     KQLEVQMNAVFDRLRYDDKSGLWENEFEYLREERTFDDYVTQPYIANASADSLVRIEV
                     TSVERLGDSAVARVLVHFDNAGRKSVIDDKVTLYWHQGKWIKPTISRLKDQAAFDSVR
                     NAAIEAAKKESGN"
     gene            3741..4175
                     /gene="mce"
                     /locus_tag="PKM94_12635"
     CDS             3741..4175
                     /gene="mce"
                     /locus_tag="PKM94_12635"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOD67521.1"
                     /translation="MASDMISHVGIAVADLDAAVARYRLLTGGAAPVIVDVPDQKVRV
                     AIFSGGEGAPGHGGGRIELVAPVSDDSPVAKFIAKRGEGLHHICLYVADIERKLAELK
                     AAGARLIDETPRCGAEGNRIAFVHPAGSHGVLLELEEHPTAL"
     gene            complement(4162..4821)
                     /locus_tag="PKM94_12640"
     CDS             complement(4162..4821)
                     /locus_tag="PKM94_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67522.1"
                     /translation="MILALVAVLALAVAGNRFVSLPDSVTLPAPGGWRVLGEPASYPA
                     VLHNEASGARLLVFETRLDPGERIGGAADFQVSVSRMIDSVVLQMPNAVLLTSTGQQA
                     ENRAEFAVEFQTIDTATGAAVYHRLAGILYRHPDGSQLLFSLWAQAPPGADAALRAEL
                     ERMQSGFRYNGPQEAQVFAPARSDMQWFLVFLIGAAAVVLFLRQRRRLARAAPEPPRA
                     P"
     gene            complement(4818..5099)
                     /locus_tag="PKM94_12645"
     CDS             complement(4818..5099)
                     /locus_tag="PKM94_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOD67523.1"
                     /translation="MFLGPTEAQLMDLAWERESLTVKQALFLLGESDGRAYTTIMTVL
                     NRLAEKGLLSRRKAGRGFIYAAAKSREEFIGERVRIVREVLRKNFAGDA"
     gene            complement(5147..5305)
                     /locus_tag="PKM94_12650"
     CDS             complement(5147..5305)
                     /locus_tag="PKM94_12650"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOD67524.1"
                     /translation="MFIGLLLVVLGLLMLLDRLGYIQGDIWGYFWPLALVALGLSMVV
                     THRRKEKR"
     gene            complement(5357..5836)
                     /locus_tag="PKM94_12655"
     CDS             complement(5357..5836)
                     /locus_tag="PKM94_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD67525.1"
                     /translation="MTPARLKLVESIRTAAADLDSETAVLRAAAELIDGYSEGYHWTG
                     FYLLRDGVLEVGPYVGPPTPHTRIALHQGICGAAASQKKSIVVDDVHADPRFLACSLT
                     TRSEIVVPLLEGGECLGEIDIDSNHPAFFTAEDREMLEAAAAVVAARLAEIRGRRPA"
     gene            complement(5833..6834)
                     /locus_tag="PKM94_12660"
     CDS             complement(5833..6834)
                     /locus_tag="PKM94_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOD67526.1"
                     /translation="MARYSHRERIEIILAGEKPDRFAGSAWRHFFHMEHDAAQTAEAM
                     VGFQRTFDWDFVKVNPRADYHVEDWGLRQEWSRNEFRKHRKTAFPVNRPEDWERIRPL
                     SPAAPVLAEHLRTVALVRKGVGREVPVLMTVFSPLAIAGRMVPDGALLAAELRANPER
                     IHRALRAITETFRAYAAEIRNAGADGLFFATTQWASRQTISWAEYEAFGIPYDREVLR
                     ATEADALNLLHVCGSENYLRELAAADYPCRLYNWDADDPTNLPLDKAYDALPGRTLAG
                     GIDRNGWFLHATPQEVVFQVQKLMRAHDGARVVLAPGCAVEPEAPRENYRAFREALG"
     gene            7112..>7581
                     /locus_tag="PKM94_12665"
     CDS             7112..>7581
                     /locus_tag="PKM94_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD67527.1"
                     /translation="MDARILVIDDEESMCRFMEIMLNREGYAVDTAASGRDGLALLRD
                     RAYDLVIADLNMPEMSGLDVLREVRSFKADQDLIVMTAYATVDTAIEAMKQGAVDYVT
                     KPFKVDAIKLTIEKALSRKRLIDENRVLKEQLAEGASFANFIGVSEPVAQLKKLA"
BASE COUNT         1292 a         2850 c         2274 g         1165 t
ORIGIN      
        1 cggtctgccg gtcacgcccg gcgcccgact cgacggcgcc ggcggggaag aggcgctcgc
       61 cgccgcgcgc caaatcggtt ttccgatcct cgtcaaggcc gccgccggcg gcggcggcaa
      121 aggcatgcgc atcgtcgccg cccccggcga gttcgccgag gctctcgaaa gcgcctcccg
      181 cgaggcgaaa tcggccttcg gcgacggccg cgtgtacatc gaaaaatacc ttgcccggcc
      241 gcgccacgtc gaaatccaga tcctctgcga ccggcacggc aacgccgtcc atcttttcga
      301 gcgcgaatgc tccatccagc gccgccacca gaaagtgatc gaggagtccc cctcgccgat
      361 gatgaccccc gagctgcggg cgcgcatggg cgacgccgcg gtccgcctcg cccgggagag
      421 cggctatgtc ggcgccggca ccgtcgaatt cctcgtcgac cgggaccgct ccttctactt
      481 cctcgaggtc aacacccgtc tccaggtcga gcacccggtg accgagatgg tgaccggcat
      541 cgatctcgtc gccgaacaga tcgcggttgc ggaaggggag cggttgcgct ggcggcagga
      601 ggacatccgc ctgcacggcc acgccctcga gtgccgcatc tacgccgagg accccgagga
      661 gggcttcctc ccctcgaccg gcaccctccg caactaccgc gctccctccg gccccggcgt
      721 gcgggttgac tccggcgtgg tcgtctactc ggaaatcccg gtctactacg acccgatcat
      781 cgccaagctg atcgtctggg ggcgcgaccg cgacgaagcc atccggcgcg ccggccgggc
      841 tctcgtggaa taccgcatcg gcggcgtcca gagcacgatc gagttccacc gcgccgtcct
      901 gcgcaacccg aaatttctcg ccggcgacct ctccacgcgc ttcctcgagg aggaatttcc
      961 ggagaacaac tacctcgtcc tcgatgacaa gctccgcgag cagatcgcga tcgcggccgc
     1021 tctcgagaca gccgcccgcg agcgcacgat tattgtccag aacaacgcgc gcggcggcgc
     1081 ggccccctcc aactgggtgc tcgtccaccg ccgctccgtc ctcaaaccgt tcagcgggag
     1141 ccgctgatgc gccgctatct cattactgtc gacggccggg aattcgacat ttcccttgac
     1201 cccagcatgc agtcggggcg cctgctgtgc aacggccgtc cccgcgaaat catcacccac
     1261 ctcctcggtc ccagccgggc gctcgtcctc atcgacaacg aatcgctcga ggtcgatgtc
     1321 cgggcgaacg cctacgacac caagaagacg ctcttcgtcc acggcatgga aatcccggtc
     1381 accattgagg actaccacct cgcgcagttg cgcaagaccg ccggcatccg ctccggcgcc
     1441 gagatcgacc gcgttctgcg cgccgccatg cccggcctgg tgctggaagt caaagtccgg
     1501 ccgggggaga tcgtccgcag gagccagccc ctgctcatca tcgaggccat gaaaatggaa
     1561 aatatcatca aggcgcaggg ggagggaacc gtcaagaccg tcttcgtcgc ccccggcaag
     1621 tcggtcgaga aaaacgacaa actcctggag ttcgcgtgat gtctgacgat acgaaactga
     1681 ccacctcggg gattcccatt ccggtcacgg cgacggccgc cgacgcgccc gctgcccccg
     1741 gtccccggca cgccggcgag tacccgttca cccgcggcgt ctacccgacc atgtaccgcg
     1801 gccgcctctg gaccatgcgc cagtacgccg gcttcggcac cgccgccgaa accaaccgcc
     1861 gtttcaagta cctcctccag cgcgggcaga ccggcctctc ggtcgccttc gacctcgcca
     1921 cccagatcgg ctacgattcc gaccacccca tggccgccgg cgaggtgggc cgcacgggag
     1981 tcgccattga ctcgctcgcc gacatggaga agctcttcga cggcatcccg ctggacaagg
     2041 tctccacctc catgaccatc aacgccccgg ccgtcatcct gctcgccatg tacatcgccg
     2101 tcggccgcaa gcagggcgtg cccgcggcgc agctctccgg cactgttcag aacgacgtgc
     2161 tcaaggaatt catcgcccgc ggcacctaca tcctgccgcc gaaaccctcg atgaagatca
     2221 tcaccgacat cttcgcctac gcggcgaagc acctgccgaa gtacaacacg atctcgattt
     2281 ccgggtacca catccgggag gcgggctcga ccgcggcgca ggaggttgcc ttcaccctgg
     2341 ccaacgccgt cgcctatgtc gaggcggcgc tcgccgccgg cttgtcgatc gacgacttcg
     2401 cgccgcgcct gtcgtttttc ttcgccgccc acaacgacct cttcgaggag gtggcgaaat
     2461 tccgcgccgc ccggcagatc tgggccgagc tcgtccgcga ccgcttccgt tcccgggatg
     2521 aaaagtcgat gctcctgcgg ttccacacgc agaccggcgg ttccaccctc accgcccaac
     2581 agcccgagaa caacatcgtg cgcacagcca tccaggccct cgccggcgtt ctcggcggca
     2641 cccagtcgct gcacacgaac tcgtacgatg aggctctcgg cctgccgacc gagaagtcgg
     2701 tggagatcgc cctgcgcacc cagcagatca tcgcccacga atccggggcc gccgccaccg
     2761 tcgacccgct cggcggctct tactacatcg agtacctgac ggccgcgatg gacaaacgca
     2821 tccgggcgct cctggccgag atcgagtcgc gcggcggcgc agtcgcctgc attgagaacg
     2881 ggtatttccg cgacgaaatc gccgccgccg cctacgactt ccagcgccgc gtcgagaccc
     2941 gccgccagat cgtcgtcggc gtcaacgagt tcacctcgca cagccccggt atcccggccg
     3001 tcctcagggt cgatcccgaa ctggaacgca accaggtccg ggaactggcc aaactcaaag
     3061 cgacccgcga ccgcaaagcc gtcgccgaat cgctggccgc cgtgcgcgac gccgcccggc
     3121 agaacaagaa cgtggtcgaa ccgatcatcg acgccgtcgc ccactacgcc actgtcggcg
     3181 aaatctccga catcttccgc gaggtatggg gtgagtacca tgaaaacctt taagctccgc
     3241 cgcctggcgt tcgccggcgc gctcctggcc ctcgtcggct gcggcggcca aagcgacacc
     3301 gacacggccg actcggccgc ggccgccggg gccgattccc tgaccctgga taagcagctc
     3361 gaggtccaga tgaacgccgt cttcgaccgc ctgcgctacg acgacaagtc cggcctctgg
     3421 gaaaacgagt tcgaatacct gcgtgaggag cgcaccttcg acgactacgt cacgcagccc
     3481 tatatcgcga acgcctcggc cgactccctc gtccgcatcg aggtgacctc ggtggagcgc
     3541 ctcggcgact cggccgtcgc ccgcgtgctc gttcacttcg acaacgccgg gcggaaatcg
     3601 gtgatcgatg acaaggtcac gctctactgg caccagggga agtggatcaa accgacgatc
     3661 tcccgactga aagaccaggc cgctttcgac agcgttcgca acgccgcgat tgaggccgcc
     3721 aagaaggagt ccggaaactg atggcttcag acatgatctc ccacgtcggc atcgcggtcg
     3781 ccgacctcga cgccgccgtc gcccggtacc gcctcctcac cggcggcgcg gcccccgtca
     3841 ttgtggacgt ccccgaccag aaagtgagag tcgcgatctt ctccgggggg gaaggcgctc
     3901 ccggccacgg cggcggccgc atcgaactgg tcgcccccgt ctccgacgac agcccggtgg
     3961 ccaaattcat cgccaaacgc ggcgagggcc tccaccacat ctgcctctat gtcgcggaca
     4021 tcgagcgcaa actggccgaa ctgaaagcgg ccggggcgag actcatcgac gagactcccc
     4081 gctgcggcgc cgagggaaac cgcatcgcct tcgtccaccc ggccggcagc cacggcgtcc
     4141 tgctcgaact ggaggaacac cctacggcgc tctaggcggc tcgggcgccg cccgcgcgag
     4201 gcgccgccgc tgccgcaaga acagcaccac cgccgccgct ccgatgagga agacgagaaa
     4261 ccactgcata tcggagcgcg ccggcgcaaa tacctgcgcc tcctgcggcc cgttgtagcg
     4321 gaaacccgac tgcatccgct ccagctccgc ccgcagcgcc gcgtccgccc cgggcggggc
     4381 ctgcgcccac aggctgaaca ggagctgcga tccgtcggga tggcggtaga ggatccccgc
     4441 cagccggtgg tacaccgccg cccccgtcgc cgtgtcgatc gtctggaact cgaccgcgaa
     4501 ttcggcccgg ttctctgcct gctgccccgt gctcgtcagc agcacggcgt tcggcatttg
     4561 gaggaccacg ctgtcgatca tccggctgac cgagacctgg aaatcggccg ccccgccgat
     4621 ccgttccccc gggtcgaggc gggtctcaaa gactagcagc cgcgccccgc ttgcttcgtt
     4681 gtgcagcacc gccggatacg acgccggctc gcccagcacc cgccagcccc cgggggccgg
     4741 cagcgtgacg gagtcgggga ggctgacgaa gcggttgccc gccaccgcca gcgccagcac
     4801 cgcgacgaga gcgaggatca tgcgtcccct gcgaaattct tgcggaggac ctcccggacg
     4861 atccgcaccc gctcaccgat gaattcctcc cggctcttcg cggccgcgta gatgaacccc
     4921 cggccggctt tgcgccgcga gagcagcccc ttttcggcca gccggttcag caccgtcatg
     4981 atcgtcgtgt acgcccgccc gtcgctctct cccaggagaa acagcgcctg cttgaccgtg
     5041 aggctctccc gctcccaggc caggtccatg agctgcgcct cggtcggtcc caggaacacc
     5101 tcgagcccgc gcccccgcgg gtcaaaatgg aaatgcccgc ccgccgtcag cgtttctcct
     5161 tgcgccggtg cgtcacgacc atcgacagac ccagcgccac caacgccagc ggccagaaat
     5221 acccccagat gtcgccctgg atgtacccga gacggtccag gagcatgagc agtccgagca
     5281 cgaccagcag caatccgata aacatgccga cctctcctgc ttacggtgtt cgatcccgtc
     5341 cgggcgcggc gccgtttcag gccggccgcc gcccccggat ttccgccagc cgcgccgcca
     5401 cgaccgccgc cgccgcttcc agcatctcgc ggtcctcggc cgtgaaaaac gcgggatgat
     5461 tcgagtcgat atcgatttcc cccaggcact ccccgccctc caggagcggc accacgatct
     5521 ccgaccgcgt cgtcagcgag catgcgagaa accgcgggtc ggcgtgcacg tcgtccacca
     5581 cgatcgattt cttctgcgat gccgccgcgc cgcagattcc ctggtgcagc gcaatccgcg
     5641 tgtgcggcgt cggcggcccc acgtacggcc ccacctcgag caccccgtcc cgcaacaggt
     5701 agaaccccgt ccagtggtag ccttccgagt aaccgtcgat cagttccgct gcggcccgca
     5761 ggacggccgt ctccgagtcc aggtcggccg ctgccgtccg gatgctctcc accagcttaa
     5821 gccgagcggg cgtcatccca gcgcctcccg aaaggcgcgg taattttccc gaggcgcctc
     5881 cggctccacc gcgcacccgg gcgcgaggac cacccgcgcg ccatcgtgcg cgcgcatcag
     5941 tttctgcacc tggaaaacca cctcctgcgg cgtcgcgtgg aggaaccacc cgttgcggtc
     6001 gatgccgccg gcgagcgtcc gccccggcag cgcgtcgtac gccttgtcga gcggaaggtt
     6061 ggtcggatcg tcggcgtccc agttgtacag ccggcagggg tagtccgcgg ccgccagctc
     6121 ccggaggtag ttctcgctcc cgcacacgtg gagcaggttg agggcgtcgg cttcggttgc
     6181 ccggagcacc tcgcggtcgt agggtatgcc gaaggcttcg tactccgccc aggagatcgt
     6241 ctgccgcgag gcccactggg tggtggcgaa aaacagcccg tccgccccgg cgttgcggat
     6301 ttccgccgcg tacgctcgga aagtctcggt gatggcccgc agcgcccggt ggatccgctc
     6361 cgggttcgcc cgcagttcgg ccgccaggag cgccccgtcc ggcaccatcc gccccgcaat
     6421 cgccagcggc gaaaagaccg tcatcagcac cggcacctcc cggcccaccc ccttgcgcac
     6481 cagcgccacc gttctgaggt gttccgccag caccggcgcg gcgggggaga gggggcggat
     6541 ccgctcccag tcctccggcc gattgaccgg gaaagccgtc ttccggtgct tgcggaactc
     6601 gttccgcgac cactcctggc ggagtcccca gtcttcgacg tggtagtcgg cccgcgggtt
     6661 gaccttgacg aagtcccagt cgaaggttct ctgaaagccc accatggcct cggccgtctg
     6721 cgcggcgtcg tgctccatgt ggaaaaagtg ccgccaggcc gagccggcga accggtccgg
     6781 tttctcgccc gccagtatta tttcgatccg ttcgcggtgg gagtacctgg ccatcgggtg
     6841 tcctctcgtc cgggtcggct gccggcgccg cttccgtttg cgggaggcgc gggtcccgcc
     6901 cgccggctcg cccggccggg cttgcgcgcc cggccgagag cgcccggttt cactcggaaa
     6961 cgacccaata tacgccgccc ccccgccctc gtcaacggcc tgtttaatcc gtgcgcaatc
     7021 gccgccgtcc gcctataatg tccgccccgc ctgccgaaac tatcctatga tacggtcaat
     7081 ccggcaacag gcagcatcgc tgggagaacc catggacgcg cgcattctgg tgatcgacga
     7141 cgaagaatcc atgtgccgat tcatggagat catgctcaac cgcgagggct acgcggtcga
     7201 caccgcggct tccggccgcg acggtctcgc gctgctgcgc gaccgcgcct acgacctcgt
     7261 catcgccgat ctcaacatgc ccgaaatgtc cggcctcgac gtcctgcgcg aggtccgctc
     7321 cttcaaggcc gaccaggacc tcatcgtcat gaccgcctac gccaccgtcg acaccgccat
     7381 cgaagccatg aaacaggggg ccgtcgacta cgtcaccaaa cctttcaagg tcgacgctat
     7441 caagctcacc atcgagaaag ccctcagccg caaaaggctc atcgacgaga accgggtgct
     7501 caaggaacag ctcgccgagg gggcgtcttt cgccaacttc atcggcgtgt ccgagccggt
     7561 cgcgcaattg aagaagcttg c
//
LOCUS       DAOPOE010000129         7543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_10868_length_7543_cov_5.245726, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000129 DAOPOE010000000
VERSION     DAOPOE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10868_length_7543_cov_5.245726"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKM94_12670"
     CDS             <1..417
                     /locus_tag="PKM94_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOD67528.1"
                     /translation="EIARTEKYRDCRVPLHTLRADIDYATSTANTTYGTIGVKVWICK
                     GEVLDRGKYVQHEEEARADEQAPSDTRPRRPRGDKAGRKRTRPRGKVRRAGAPGPEGP
                     PPKGRRPEGPARPRGEGRPPAPDGREGGAGESGAPE"
     gene            447..869
                     /gene="rplP"
                     /locus_tag="PKM94_12675"
     CDS             447..869
                     /gene="rplP"
                     /locus_tag="PKM94_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOD67529.1"
                     /translation="MLMPNKVKFRKQQRGRMRGKAKGGAEVSFGEYGLKAMEPCWLTN
                     RQIEAARIAMTRSIKRGGKIWIRIFPDKPVTKKPAETRMGKGKGAPEYWVAVIKPGRI
                     LFEIQGVTEASAREALRLGANKLPLKTKFVSRSETEGV"
     gene            873..1124
                     /gene="rpmC"
                     /locus_tag="PKM94_12680"
     CDS             873..1124
                     /gene="rpmC"
                     /locus_tag="PKM94_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOD67530.1"
                     /translation="MLKVHSLREMTRDELVQKKNELLDEQFNLRMRRSLKALDNPLRL
                     RQIRREIAKIQTVLHEDATGRRKLADMKTSILPPSGKKE"
     gene            1150..1416
                     /gene="rpsQ"
                     /locus_tag="PKM94_12685"
     CDS             1150..1416
                     /gene="rpsQ"
                     /locus_tag="PKM94_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005872739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOD67531.1"
                     /translation="MAETVERNHRKIRQGTVVSDKMNKTIVVRLDRTRPHALYNKTSK
                     STTKLYAHDENNDARPGDLVRVMETRRLSHKKRWRLIEIVERAK"
     gene            1468..1833
                     /gene="rplN"
                     /locus_tag="PKM94_12690"
     CDS             1468..1833
                     /gene="rplN"
                     /locus_tag="PKM94_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOD67532.1"
                     /translation="MVQEYTILNVADNSGAKKVMCFRILGGRKKYASIGDVIVVAVKE
                     AIPGGTVKKSEVCRAVVVRTKASLRRKDGSLIRFSDNAAVIINDQLEPRGTRIFGPVA
                     RELREKKFMKIVSLAPEVL"
     gene            1852..2169
                     /gene="rplX"
                     /locus_tag="PKM94_12695"
     CDS             1852..2169
                     /gene="rplX"
                     /locus_tag="PKM94_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOD67533.1"
                     /translation="MRIKKGDTVYVLCGEDKGKTGRVLHVYPKKGTAVVEGVRMHKRH
                     QRPTQRNPKGGVVTMEGPIHLSNLAVYNASSGGPARLGVRTTEEDGRKRRVRIDVKTG
                     EEI"
     gene            2181..2720
                     /gene="rplE"
                     /locus_tag="PKM94_12700"
     CDS             2181..2720
                     /gene="rplE"
                     /locus_tag="PKM94_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOD67534.1"
                     /translation="MARYRELYQKEVVPKLMKQNGYRSIMQVPRITHVTVNIGVGEAT
                     DNSKALDAAVEDLAKITGRKPLVTRARKSISNFKLRKGMPIGCTVTLRRDAMYDFLDR
                     LVNVAIPRIRDFRGVNPKSFDGRGNYNLGLKEQLIFPEIDLDKVDKVRGMNISIATTA
                     RTDQEARQLLEELGMPFRK"
     gene            2728..2913
                     /locus_tag="PKM94_12705"
     CDS             2728..2913
                     /locus_tag="PKM94_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOD67535.1"
                     /translation="MAKKSLIEKSNRKPKFQVRAYHRCRRCGRPRAYLRRFGLCRICF
                     RQLALAGELPGVVKASW"
     gene            2942..3337
                     /gene="rpsH"
                     /locus_tag="PKM94_12710"
     CDS             2942..3337
                     /gene="rpsH"
                     /locus_tag="PKM94_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOD67536.1"
                     /translation="MTDPIADLLTRIRNGSKAKKNAVDVPASNLKREVVRILREHRFI
                     KDVVDLPDNKQGILRVYLRYVAGDTPVLKGIQRLSRPGLRRYFDAETVRQSTFNTRGM
                     LVLSTSSGVMTNFEAARKGVGGEVLLRCW"
     gene            3351..3890
                     /gene="rplF"
                     /locus_tag="PKM94_12715"
     CDS             3351..3890
                     /gene="rplF"
                     /locus_tag="PKM94_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOD67537.1"
                     /translation="MSRIGKQPIAVPDKATVSITGRQVTIKGPKGSLTLEVHPDMTAS
                     LDHNQIVVARPSDLKKHRALHGLTRALLHNMVVGVTQGFTRELEIVGVGYRAENRGRH
                     LVMYLGYSHPVVLTPPEGVTVEAVPKEGKVRVSGIDKQLVGQVAAKIRSFRKPEPYKG
                     KGVRYAGEHVRTKAGKTAG"
     gene            3905..4279
                     /gene="rplR"
                     /locus_tag="PKM94_12720"
     CDS             3905..4279
                     /gene="rplR"
                     /locus_tag="PKM94_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOD67538.1"
                     /translation="MAGKSVKKFKRAERRRQRVRGKLSGTSACPRLTVAKSLKNVFVQ
                     IVDDVHAVTLAAAASNSKNVLSELKPEMTKSEVARKVGEVIAREAKAKGIEAVVFDRN
                     RFRYHGRVKAVAEGARSGGLKF"
     gene            4318..4800
                     /gene="rpsE"
                     /locus_tag="PKM94_12725"
     CDS             4318..4800
                     /gene="rpsE"
                     /locus_tag="PKM94_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOD67539.1"
                     /translation="MARFEMENLELEEKVISVKRVAKVVKGGRRFSFTALVAVGDKNG
                     RVGVGHGKAPEVSDAIRKGTEAARKNMTSVNLLKGTIPHRIDGEFGATRVVMRPASPG
                     TGVIAGGAVRAVLESVGIQDILAKTLGSRNPHNMVKATLNGLAALQTREQEVKARQAE
                     "
     gene            4827..5015
                     /gene="rpmD"
                     /locus_tag="PKM94_12730"
     CDS             4827..5015
                     /gene="rpmD"
                     /locus_tag="PKM94_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOD67540.1"
                     /translation="MGKLRVTQIRSTIDRKEPHKRTIEALGLGKIRRSVIHNDTPQIR
                     GMIRAVRYLVTVEEIDEK"
     gene            5025..5465
                     /gene="rplO"
                     /locus_tag="PKM94_12735"
     CDS             5025..5465
                     /gene="rplO"
                     /locus_tag="PKM94_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOD67541.1"
                     /translation="MELHTLKPPKGARKTRRRVGRGSGSGLGKTSGRGHKGAGARSGF
                     KREAWREGGQMPLARRLPKFGFTNIFKKTWQVINLSDLARLEAGEVTPDTMLAAGLIK
                     TTAVPVKVLGDGTVEKAYTVKAAAFSKSAASKIEAAGGKAEVLS"
     gene            5462..6772
                     /gene="secY"
                     /locus_tag="PKM94_12740"
     CDS             5462..6772
                     /gene="secY"
                     /locus_tag="PKM94_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOD67542.1"
                     /translation="MIDKFRSVFKVEELRRRIFYTLALLVVYRIGGHIPTPGIDGSIL
                     ASVLANNTIFGLFDLFVGGAFAKATIFAMGIMPYISASIMLQLLGAVVPYLQRLQREG
                     EEGRRKITQYTRYLTVLIAALQAWGTAGYLTTIKSAAGVSAVHMNTAAFIPLTILTFT
                     SGTIFIMWLGEQITERGIGNGISLIIFIGIIARFPEAVVQEVLLVWHGSRNLFVGAIM
                     LAAMVAVIAAVILVTRAQRRVPVQYPRKVVGRRVFGGATTHLPLSVNSAGVIPIIFAQ
                     SIMFLPSTVAQLFPNTDWVQNLVAVWLAPGGLWYSLIYGVIIIFFAYFYTAIVFNPME
                     IADNMRKNGGYIPGIRPGKNTADYIEKILTRITLPGAIFFAAIAILPWVLIGQFNVNF
                     FFGGTGLLIVVGVALDTLQQIESHLIMRHYDGFMKKGRIRGRSM"
     gene            6772..7425
                     /locus_tag="PKM94_12745"
     CDS             6772..7425
                     /locus_tag="PKM94_12745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOD67543.1"
                     /translation="MRHLVFLGPPGSGKGTQAAKLAAEMKIRYLSTGDLLRAAVSTGT
                     ELGRKARGYMERGELVPDALLVGLIEATIRAGELDHGFILDGFPRTVPQAEALRDMLN
                     GNGKELDAAVLLAVSDEEVVRRLSGRWFCPSCQATYNYPHHMPRREGRCDAEGAALER
                     RPDDQPEVVRRRLDVYKKQTRPIEDFYRAAAILVEVQGEQPPEAVFADLLDIVGARK"
BASE COUNT         1521 a         2208 c         2697 g         1117 t
ORIGIN      
        1 gagatcgcgc gcaccgagaa gtaccgggac tgccgggtgc cgctccacac cctgcgggcg
       61 gacatcgact acgccacctc gacggcgaac acgacctatg ggacgatcgg ggtgaaggtg
      121 tggatctgca agggggaggt gctcgaccgg ggcaagtacg tacagcacga ggaagaggcg
      181 cgggccgacg agcaggcgcc gagcgacacc cgtccccgcc gtccgcgggg cgacaaggcc
      241 ggccgcaagc ggacccgtcc gcgcggcaaa gtgcggcgcg ctggcgcccc cggtcccgag
      301 ggcccgccgc ccaagggccg tcggccggaa ggtccggccc gcccgcgcgg cgaagggcgc
      361 ccgccggccc ccgacggccg ggagggcggc gccggtgaat caggagcccc cgagtgagtc
      421 ggagcgagaa ggtggagtag agtcccatgt taatgccgaa caaagtcaag tttcgcaagc
      481 agcagcgcgg ccgcatgcgg gggaaagcca agggcggggc ggaagtcagt ttcggcgagt
      541 acggcctgaa ggccatggag ccgtgctggc tgaccaaccg ccagatcgag gccgcccgta
      601 tcgccatgac ccgctcgatc aagcgcggcg gaaagatctg gatccggata ttcccggaca
      661 agccggtgac caagaagccg gccgagacgc gcatggggaa agggaagggg gcgccggagt
      721 actgggtggc cgtgattaag cccggacgca tcctcttcga gatccagggg gtaaccgagg
      781 cctcggcgcg ggaggccctg cgcctgggcg ccaacaagct gcccttgaag acgaagttcg
      841 tgtcgcgttc cgagacggaa ggagtgtaag cagtgttgaa ggtacattcg ctccgggaga
      901 tgacgcgcga cgagctggtg cagaagaaga acgagcttct ggacgagcag ttcaacctgc
      961 ggatgcggcg gtcgctcaag gcgctcgaca acccgctccg gctgcggcag atccggcggg
     1021 agatcgcgaa aattcaaacg gtgctgcacg aggatgcgac gggccggcgg aaactggccg
     1081 acatgaagac ctcgattttg ccgccctcgg gcaagaaaga ataagcggga tcgctgagag
     1141 gaatggcaga tggcagaaac ggttgagaga aaccaccgca agatccgtca ggggacggtc
     1201 gtgtcggaca agatgaacaa gacgatcgtg gtgcggctgg accggacgcg tccccacgcg
     1261 ctctacaata agacgtcaaa gtcgacgacc aagctgtacg cgcacgatga gaacaacgac
     1321 gcccggccgg gcgacctggt gcgggtaatg gagacgcggc ggctgtcgca caagaagcgc
     1381 tggcgcctga tcgagatcgt agaacgggcc aagtagcgcg gccggatggc ccttggcaca
     1441 ggccgggcgc cgcagaggtg agtagagatg gtgcaggaat atacgatatt gaacgtggcc
     1501 gacaactccg gcgcgaaaaa ggtgatgtgc ttccgcatcc tcgggggacg caagaagtat
     1561 gcctcgatcg gcgacgtcat tgtggtcgcg gtgaaggagg cgatccccgg ggggacggtg
     1621 aagaagtcgg aggtctgccg ggcggtggtc gtgcgcacga aagcgtccct gcggcgcaag
     1681 gacggctcgc tcatccggtt ctcggacaac gcggccgtga tcatcaacga ccagctcgag
     1741 ccccggggca cccgcatctt cgggcccgtg gcccgcgaac tgcgcgagaa gaaattcatg
     1801 aagatcgtct ccctcgcccc cgaggtgttg taggcggagg aagagcagcg tatgcgaatc
     1861 aagaaaggcg atacggtcta cgtcctctgc ggagaggaca aagggaaaac cggccgggtg
     1921 ctccacgtgt acccgaagaa ggggacggcg gtggtggaag gcgtgcgcat gcacaagcgg
     1981 caccagcggc cgacccagcg gaacccgaag ggcggggtgg tgacgatgga aggtccgatt
     2041 cacctgagca acctcgccgt gtacaatgcc tcgtcgggcg gcccggcgcg gctgggggtc
     2101 cggaccacgg aagaagacgg ccgcaagcgg cgcgtccgga tcgatgtgaa gacgggcgag
     2161 gagatctaag gaaaacggcg atggcgaggt acagagagct ctaccagaag gaagtggtgc
     2221 cgaagctgat gaagcagaac ggctaccggt cgatcatgca ggtgccgcgc atcacgcacg
     2281 tgacggtgaa tatcggcgtg ggagaggcga cggacaactc gaaggcgctc gacgcggccg
     2341 tggaggacct ggcgaaaatc acggggcgca agccgctcgt gacgcgggcc cgcaagagca
     2401 tctcgaattt caagctgcgc aagggaatgc cgatcgggtg cacggtgact ttgcggcggg
     2461 acgcgatgta cgattttctc gaccgcctcg tcaacgtcgc catcccccgc atccgcgact
     2521 tccgcggcgt gaacccgaag tcgttcgacg ggcgggggaa ctacaatctc ggattgaaag
     2581 agcagttgat ctttccggag atcgatctgg acaaggtgga taaagtgcgc gggatgaaca
     2641 tttcgatcgc gacgacggcg cggacggacc aggaggcccg tcagctgctc gaggagctgg
     2701 gaatgccgtt ccgcaagtag aggtgacatg gcgaagaaat ccctcataga gaagtcgaac
     2761 cgtaagccga aattccaggt gcgggcgtac caccggtgcc gccggtgcgg ccgtccgcgg
     2821 gcctacctgc gccgcttcgg gctgtgccgg atctgtttcc ggcagctcgc cctggccggc
     2881 gaattgcccg gcgtggtgaa agccagctgg tgaggaaact gggagcgaaa gcacaatgag
     2941 catgacagat ccgatagccg atcttctcac gcgcatccgg aacgggtcga aagcgaagaa
     3001 gaatgcggtc gatgtgcccg cgtcgaacct caagcgcgag gtggtgcgga ttctccgcga
     3061 gcaccgcttc atcaaggacg tcgtcgacct gccggacaac aagcagggaa tcctccgggt
     3121 ctacctgcgc tacgtggccg gcgacacgcc ggtgctcaaa ggaatccagc gcctgtcgcg
     3181 gccgggcctg cggcggtact tcgacgcgga gacggtgcgg cagtcgacgt tcaacacgcg
     3241 cggcatgctg gtgctctcga cgtcgagcgg cgtgatgacg aactttgagg cggcccggaa
     3301 aggcgtgggc ggcgaagtgc tgctgcgctg ctggtaggaa aggacgagag atgtcgcgaa
     3361 ttggcaagca gccgatcgct gtgccggaca aggcgacggt gagcatcacc gggcgccagg
     3421 tgacgatcaa ggggccgaag ggcagcctca ctctggaggt gcacccggac atgacggcca
     3481 gcctggacca caaccagatt gtcgtcgccc gcccgagcga tctgaagaaa caccgggccc
     3541 tccacggcct cacgcgggcg ctcctgcaca acatggtcgt gggagtgacc caggggttca
     3601 cccgcgaact cgaaattgtc ggcgtcggct accgcgccga aaaccgcggc cggcacctcg
     3661 tgatgtacct cgggtactcg cacccggtgg tcctcacccc gccggagggc gtgactgtcg
     3721 aagccgtgcc caaagagggc aaagtcaggg tctcggggat agacaagcaa ctcgtcggcc
     3781 aggtggcggc gaagattcgg tcgttccgga aaccggagcc gtacaagggc aagggtgtcc
     3841 ggtacgccgg cgaacacgtg cggacgaagg ccgggaagac cgccggataa gagagtagac
     3901 ggaaatggct ggaaagagtg tcaagaagtt caagcgggcg gaacgccgcc gccagcgggt
     3961 gcggggcaag ctgtcgggaa cctcggcgtg cccgcgcctg acggtcgcga agtcgctgaa
     4021 gaacgtgttc gtgcagattg tcgacgacgt ccacgcggtg acgctcgcgg cggccgcctc
     4081 caattccaaa aacgtcctct ccgagctcaa gccggagatg accaagtccg aagtggcgcg
     4141 gaaagtgggc gaagtgatcg cgcgcgaggc gaaggcgaag gggatcgagg cggtggtctt
     4201 cgaccgcaac cgattccgct accacggccg ggtcaaagcg gtcgcggagg gggcgcgctc
     4261 gggtggcctg aagttctgat aacgaataag tgtgcatagg acaagctgag gtgcagattg
     4321 gcgcgttttg agatggaaaa cctcgaactc gaggaaaagg taatctcggt caagcgggtg
     4381 gcgaaagtgg tcaagggcgg ccggcggttt tcgtttaccg cgctggtggc ggttggcgac
     4441 aagaacggcc gggtcggcgt gggccacggc aaggcccccg aggtatccga tgcgatccgc
     4501 aaggggacgg aggcggcgcg caagaacatg acctccgtga acctgctcaa ggggacgatc
     4561 ccgcaccgga tcgacggcga gttcggcgcc acccgggtgg tcatgcggcc ggcctcgccc
     4621 ggcaccggcg tgatcgccgg cggcgccgtg cgggccgtgc tcgagtcggt gggtattcag
     4681 gacatcctgg ccaagacgct ggggagccgc aacccgcaca acatggtcaa ggcgacgctg
     4741 aacgggttgg ccgccctgca gacgcgcgaa caggaagtca aggcgcgcca ggccgagtag
     4801 gcgtgccggc agagaggacg accgcaatgg gcaagcttag agtgacgcag atcagaagca
     4861 cgatcgaccg caaggagccg cacaaacgca ccatcgaggc gctggggctc ggcaaaatcc
     4921 ggcggtccgt catccacaac gacacgccgc agatccgcgg tatgatccgc gccgtccgct
     4981 atctcgtgac ggtggaagag atcgacgaaa aatagaggcg agtgatggaa ctgcatacgt
     5041 tgaaaccgcc gaaaggggca agaaagacgc gcaggcgggt gggacgcggc tcgggcagcg
     5101 gcctggggaa aacctcggga cgcggccaca agggggccgg ggcgcggagc ggcttcaagc
     5161 gcgaagcctg gcgcgaaggc ggccagatgc cgctcgcacg gcgcctgccc aaattcggtt
     5221 tcaccaatat cttcaagaag acctggcagg tgatcaacct gtccgacctc gcgcgcctcg
     5281 aggcgggcga ggtgacgccc gatacgatgc tcgcggcggg cctgatcaag acaacggccg
     5341 taccggtgaa agtgctgggg gacgggacgg tggagaaggc gtacacggtc aaagcggcgg
     5401 cgttcagtaa gtcggcggcc agcaagatcg aagccgccgg cggaaaagcc gaggtgctgt
     5461 cttgatagat aagttcagat cggtgttcaa agtcgaggag ttgcgaagac ggatcttcta
     5521 caccctcgcg ctgctcgtcg tctaccgcat cggcggacac atccccacgc ccgggatcga
     5581 cggcagcatc ctggcctcgg tgctggccaa caacaccatc ttcgggctgt tcgacctgtt
     5641 cgtgggcggg gccttcgcca aggcgactat tttcgccatg ggcatcatgc cctacatctc
     5701 ggcctcgatt atgctccagc tcctcggcgc ggtggtgccg tacctccagc ggctgcagcg
     5761 cgagggcgag gaggggcggc gcaagatcac gcagtacacg cggtacctga ccgtgctcat
     5821 tgccgcgctc caggcgtggg ggacggcggg gtacttaacg accatcaaga gcgcggcggg
     5881 cgtctcggcc gtgcacatga acacggcggc gttcatcccc ctcaccatcc tcacgttcac
     5941 gagcggcacg attttcatca tgtggctcgg cgagcagatc acggagcggg ggatcgggaa
     6001 cggtatttcg ctgatcattt tcatcgggat catcgcgcgc ttccctgagg ccgtggtgca
     6061 ggaagtgctg ctggtgtggc acgggtcacg caacctgttc gtgggcgcga tcatgctggc
     6121 ggccatggtg gccgtgatcg cggcggtcat tctggtgacg cgggcgcagc gccgggtgcc
     6181 ggtgcagtat ccgcgcaagg tggtgggccg ccgggtgttc ggcggggcga cgacgcacct
     6241 gccgttgtcg gtgaactcgg ccggcgtcat cccgatcatc ttcgcccagt cgatcatgtt
     6301 tctcccctcg accgtcgcgc agctgttccc caacaccgac tgggtgcaga acctggtggc
     6361 ggtgtggctg gcgccgggcg gcctctggta ctccctcatc tatggggtga tcatcatctt
     6421 cttcgcctac ttctacacgg cgatcgtgtt caaccccatg gagatcgccg acaacatgcg
     6481 caagaacggc gggtacattc caggcattcg gccgggcaag aacaccgccg actatattga
     6541 gaaaatcctc acccggatta ctctgccggg cgccatcttc ttcgccgcca tcgccatcct
     6601 cccctgggtg ctcatcggcc agttcaacgt gaacttcttc ttcggcggca ccgggctcct
     6661 catcgtggtc ggcgtggccc tcgacaccct ccagcagatc gagtcgcacc tgatcatgcg
     6721 gcactacgac gggttcatga agaaagggcg aatccgggga aggtccatgt gatgaggcat
     6781 ctcgtgtttc tcggcccgcc cggatcgggc aaagggacgc aggcggcgaa gctggcggcg
     6841 gagatgaaga tccgctacct gtcgaccggg gacctgctgc gcgccgcggt gagcaccggg
     6901 accgagctgg gccgaaaagc gcgagggtat atggagcgcg gggaactcgt cccggatgcg
     6961 ctcctcgtgg gattgatcga ggcgaccatc cgggccgggg aactcgacca cggattcatt
     7021 ctggacgggt ttccgcggac ggtgccgcag gcggaggcgc tgcgggatat gctgaacggc
     7081 aatggcaagg aactcgatgc ggccgtcctc ctggccgtgt cggacgagga agtggtgcgg
     7141 cggctgtcgg ggcggtggtt ctgcccgtcc tgccaggcga cctacaacta cccgcaccac
     7201 atgccccggc gggagggccg gtgcgacgcg gagggcgcgg ccctggagcg gcgcccggac
     7261 gaccagcccg aggtcgtgcg ccggcgactg gatgtgtaca agaaacagac gcggccgatc
     7321 gaagatttct accgggcggc ggccattctg gtcgaggtgc agggggaaca gccgcccgag
     7381 gcggtgtttg ccgacctgct cgacatcgtc ggggcgcgaa aataggacgg aagcgacgtt
     7441 tgtgatagtg ctgaagacgg cggaacagat cgaggtgatg gcccgggcgg gccgggtggt
     7501 cgggcggacg ctcgacatgg tcggcaccgt catccggccg gga
//
LOCUS       DAOPOE010000130         7398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_11125_length_7398_cov_5.379954, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000130 DAOPOE010000000
VERSION     DAOPOE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_7398_cov_5.379954"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1676)
                     /locus_tag="PKM94_12750"
     CDS             complement(12..1676)
                     /locus_tag="PKM94_12750"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOD67544.1"
                     /translation="MQTALHILSLIDEIRREMVGGRVASTEFYKKERAAYLFVKGPHT
                     RVAFGFLYHPHGAGVFVVPASKVTIETKEKPWPIFDLDGALVAGVEPFGLDRLFALHF
                     RAGDTESRVVCEAMGANGNLWLLDADGRLRGTLRKREFTPGGIYAPPEPFAGLDPFAA
                     AAEALAAHLDEAPGMSAAALLQRHVTGFNRTLAVEAVHRSRTEPGDRSPEALAAVAGA
                     VRDLAERFRTPETGCLYTVGGRLEAYPFKLASVAEPPEKFKSLSLAVMQMASRRQTTV
                     EQEDEEKTVLAAVGRAIARLERRIRNIESDLAEAADFEKFRKFGELLQINFDALARGM
                     EAITVDDVYADPHTPLTIPLDPALGPAENAEVYFRRYRKGREGLELLQRRLDISRQEL
                     EELRRMQAELGRQFESARERYRADLEALLPKEGTREDAAPRLPFRAYTLSTGLTIWVG
                     KDGSDNDRTTFEYAKPHEIWLHAQQCPGSHVVIKHPNKSFVPSKQEIAEAAAIAAWFS
                     KARNDTLVPVIYTERRYVRKPRKAKPGLVTVEREKSVMVTPAKPAD"
     gene            complement(1743..2162)
                     /locus_tag="PKM94_12755"
     CDS             complement(1743..2162)
                     /locus_tag="PKM94_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD67545.1"
                     /translation="MRSPALTVDVIIEFPDGSIVLIERGHEPYKGRLALPGGFVEYGE
                     RVEEAAVREAREETGLEIRLDRLVGVYSDPARDPRGHTVSVVYHAEPIGGVLAGGDDA
                     ARAVRTRAYLGRPLAFDHARILRDAFGDRRADGTPEA"
     gene            2488..2700
                     /locus_tag="PKM94_12760"
     CDS             2488..2700
                     /locus_tag="PKM94_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOD67546.1"
                     /translation="MKYGARNQIRAKVTSVKKGDVMSLVKFQITPPAQAASVLTTESL
                     SDLKLKKGDEVILVIKAIHVLPVKDA"
     gene            complement(2779..4170)
                     /gene="fumC"
                     /locus_tag="PKM94_12765"
     CDS             complement(2779..4170)
                     /gene="fumC"
                     /locus_tag="PKM94_12765"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOD67547.1"
                     /translation="MEYRIETDTMGEMKVPADRYYGCQTARSLHNFRIGGERMPRELI
                     RAMGILKKAAALVNMELGTLPKDKGELIVRAADEVIEGKLDEHFPLVIWQTGSGTQTN
                     MNANEVISNRAIELAGGVLGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEETHRRLI
                     PMVAQLHDTLRQKSEEYRDIIKIGRTHLMDATPLTLGQEISGWTAQLALGLDRVRGAL
                     VRVYPLALGGTAVGTGLNTHPEFAERSAKTIARLTGLPFVSAPNKFEALASHDALVEF
                     HGALKTLAASLFKIANDIRWLGSGPRCGIGELILPENEPGSSIMPGKVNPTQPEAMTM
                     VCAQVMGNDVTVNIGGMSGNFELNVFKPVIIYNVLQSIRLLADACEMFNEHCAVGLRA
                     DKDRLAAHLRNSLMLVTALNPHVGYDNAAKIAKKAHAEGTTLKEAAVALGLLTPEKFD
                     EVVRPEEMIGPRR"
     gene            complement(4355..4666)
                     /locus_tag="PKM94_12770"
     CDS             complement(4355..4666)
                     /locus_tag="PKM94_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOD67548.1"
                     /translation="MNIYVGNLPFSTDEEELKAKFEAYGTVTSAKIIMDRVSGRSRGF
                     AFVEMPNDEEAQAAIDGLQGSDYGGRALTVSEARPREGGGGGGGRGGGRDRDRGDRER
                     W"
     gene            complement(4851..6443)
                     /gene="lysS"
                     /locus_tag="PKM94_12775"
     CDS             complement(4851..6443)
                     /gene="lysS"
                     /locus_tag="PKM94_12775"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD67549.1"
                     /translation="MTDRNSHPAPEPHPQSEPSDAQIPLADLIRIRYQKACDLQARGI
                     NPYPYRYDRDHEIAALLADFDTLAERQTAVRIAGRVMLIRKMGKVFFADLRDQSDRIQ
                     IYLKIDTVGEDQFRLFDAVDLGDIVGCEGTLFVTRTGERTLNVRRFELLTKALHPLPD
                     KRAGLTDVEMRYRRRYADLIANPEVRATFLKRTAIVQAIRDFFNRRGFLEVETPILQP
                     IYGGASARPFKTHHHKLDEDMYLRIADELYLKRLIVGGFEKVWEYCKDFRNEGMDRLH
                     NPEFSMVEAYWAYADYRDMARLVEELVRHVAFAVHGAYRFTYGEREIDVAPPFRWVSM
                     VDSIREAAGLDLMALSYEQARAEAKKMHLPDEDLTNWGKCVEAIWEAKVEPTLVQPTF
                     IADFPREISPLAKVHRENPRLAERFELFIAGQETANAFSELNDPVDQLRRFIEQGKAL
                     AAGDEEAQPLDDDFITALTYGMPPTAGLGLGVDRFVMLLTNQHTIREVLLFPQMKREL
                     ADGAVPVSRILARIIGADKETP"
     gene            complement(6519..7031)
                     /gene="coaD"
                     /locus_tag="PKM94_12780"
     CDS             complement(6519..7031)
                     /gene="coaD"
                     /locus_tag="PKM94_12780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOD67550.1"
                     /translation="MTKTKTKSRAVYPGTFDPITNGHMSLIERACSLFDEVIVAVALN
                     AGKNPFFEFEERIQLVEQSVRARDFSPQVKVVGFDGLLADVATDVKARAIIRGLRAVS
                     DFEYEFQMALMNRKLAREIETVFLMPALSWVYLSSTIVKDVAVHGGDVNGLVPPPVRA
                     ALRRRNKLRS"
     gene            complement(7104..>7398)
                     /locus_tag="PKM94_12785"
     CDS             complement(7104..>7398)
                     /locus_tag="PKM94_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67551.1"
                     /translation="PGRHVGERERYAVEIKTVVSFRLAALFDYGLTLPPSALVVSGGA
                     AEEALQAALQKQFTVPIERPGVNAGFLAGPLRGELEQPERYLAAMGLGANFLA"
BASE COUNT         1177 a         2408 c         2495 g         1318 t
ORIGIN      
        1 gcgctgcgct tctagtcggc cggcttggcc ggcgtgacca tcaccgattt ctcacgctcc
       61 accgtcacca gccccggctt ggccttgcgg ggcttgcgga catagcggcg ctcggtgtag
      121 atcaccggga cgagcgtgtc gttgcgggct ttcgagaacc aggccgcgat cgccgccgcc
      181 tcggcaatct cctgcttcga cggcacgaac gacttgttcg ggtgtttgat caccacgtgc
      241 gagccgggac actgctgcgc gtgcagccag atctcgtgcg gcttggcgta ctcgaaggtg
      301 gtgcggtcgt tgtcggaacc gtccttcccc acccagatcg tcagccccgt ggagagcgtg
      361 taggcgcgga aggggaggcg cggcgcggca tcctcgcggg tcccctcttt cggcagaagc
      421 gcctcgaggt cggcgcggta gcgctcgcgc gcggattcga actgccggcc gagttccgcc
      481 tgcatccgcc ggagttcctc gagttcctgg cggctgatgt cgagccgccg ctgcagcagc
      541 tccagcccct cccgcccctt gcggtatctt cggaaataga cctcggcgtt ctcggccggc
      601 ccgagcgccg gatcgagcgg gatcgtgagc ggcgtatgcg ggtcggcgta gacgtcgtca
      661 accgtgatcg cctccatgcc ccgcgcgaga gcgtcgaaat taatctgcag gagctccccg
      721 aatttccgaa acttctcgaa atcggccgcc tcggccagat cggattcgat gttccggatg
      781 cgccgctcga gccgcgcgat cgcccgcccg accgccgcga gcacggtctt ctcctcatcc
      841 tcctgctcga ccgtcgtctg gcgccgggag gccatctgca tgaccgccag cgagagggac
      901 ttgaatttct ccggcggttc ggcgaccgag gccagtttga aggggtaggc ctcgaggcgg
      961 ccgccgactg tgtagaggca gccggtctca ggggtgcgga accgctccgc caggtcgcgc
     1021 accgctcccg cgaccgcggc cagagcctcc ggcgagcggt cgccgggctc agtgcggctg
     1081 cggtggaccg cctcgaccgc gagggtccgg ttgaatccgg tgacgtgtct ctggagcagg
     1141 gcggcggcgc tcatcccggg agcttcgtcc aaatgcgcgg cgagcgcctc ggcggcggcc
     1201 gcaaacggat ccagtccggc gaacggttcg gggggggcgt agatcccgcc gggggtgaac
     1261 tcgcgcttgc gcagggtgcc gcggaggcgg ccgtcggcat cgagcagcca gaggttgccg
     1321 ttggccccca tggcctcgca cacgacgcgg gattcggtgt ccccggcgcg aaagtgaagc
     1381 gcaaacaggc ggtccaggcc gaacggctcg acgccggcga ccagcgcccc gtcgaggtcg
     1441 aaaatcggcc acggtttctc tttcgtctcg atcgtcactt tcgaggccgg cacgacgaag
     1501 accccggccc cgtgcgggtg gtacaggaag ccgaaggcga cgcgcgtgtg ggggcccttg
     1561 acgaacaggt acgccgcgcg ctccttcttg tagaactcgg tcgaggccac ccgaccgccg
     1621 accatctcgc ggcggatctc gtcgatcagg ctcaggatgt gaagggcggt ctgcatggcg
     1681 gaatatagga atcggggcgg ggatcgcaag cgttcgggag cgggcgcccg gtcgccgcgc
     1741 cactaggctt ccggcgtacc gtccgcccgg cggtccccga atgcgtcgcg gaggatccgg
     1801 gcgtggtcga acgccagcgg ccggccgagg taggcgcggg tgcggacagc gcgggcggcg
     1861 tcatccccgc cggccaacac cccgccgatc ggctccgcat ggtacacgac cgagaccgtg
     1921 tgcccgcgcg ggtcgcgcgc ggggtcggag tagacaccga ccagccgatc gaggcgaatc
     1981 tccagaccgg tttcctctct ggcctcgcgg acggccgcct cctcgacccg ctcgccgtac
     2041 tcgacgaagc cgccggggag ggcgagccgt cccttgtacg gttcatggcc gcgctcgatc
     2101 aggacaatgg aaccgtcggg gaactcgatg atcacgtcga ccgtcaaggc gggggatctc
     2161 atggctgttc tcccatttgt cgtcattcct tccgcaatat gagcggcccg ggcggttcgg
     2221 cgcaaaccct ttcgcccggg cggaaacgga tcgccggcca acgggtataa ggcggtgaag
     2281 ggccgggaaa tccggccggg tttttcgccc gaaaaacctg tcgtgtccat cgtgcttagg
     2341 gcggagcagc tccctgctcc gcttttttgt gcgacacccg cccgccgcgg ccgccgcgca
     2401 ggcggcctcc gcgccgttga caggaagcgc ctcgcggtcg tattctctct tgtgcaggaa
     2461 atcccaaccc gtggcaagga gcgagcgatg aagtacggtg cgcgcaatca gatccgggcg
     2521 aaagtcacgt ccgtgaagaa gggggacgtc atgtcgctgg tcaagttcca gatcacccct
     2581 ccggcccagg cggcctcggt gctgaccacc gagtccctca gcgacctcaa gctgaagaag
     2641 ggggatgagg tcatcctggt catcaaggcg attcacgtgc tgccggtgaa agacgcctga
     2701 gaacacccgc tggggcggcg cgataacaag gcgggagccc ggggctcccg ccttgttcat
     2761 ggccgccggt gcggccggtt atctgcgggg gccgatcatc tcctcggggc gcaccacttc
     2821 gtcgaacttc tccggcgtca gaagcccgag cgccaccgcc gcttccttga gcgtcgtccc
     2881 ctcggcgtgg gccttcttgg cgatcttggc cgcgttgtcg tacccgacgt gcgggttgag
     2941 tgcggtcacg agcatgagcg agttccgcag gtgggcggcg aggcggtcct tgtcggcccg
     3001 caggccgacc gcgcagtgct cgttgaacat ctcgcaggcg tcggcgagca gccggatcga
     3061 ctggaggaca ttgtagatga tcaccggttt gaagacgttc agttcgaaat tgccggacat
     3121 tccgccgatg ttgactgtga cgtcgttgcc catcacctgc gcgcacacca tggtcatcgc
     3181 ttccggctgg gtcgggttga ccttgccggg catgatcgag gacccgggtt cgttttcggg
     3241 gaggatcagt tcgccgatgc cgcaccgggg cccggagccg agccagcgga tgtcgttggc
     3301 gattttgaac agggaggccg ccagcgtctt gagcgcgccg tggaactcca ccagcgcgtc
     3361 gtggctcgcg agcgcctcga atttgttcgg cgccgacaca aagggcaggc cggtcaggcg
     3421 ggcgatcgtt ttcgccgacc gttcggcgaa ttccggatgg gtgttcagac ccgtgccgac
     3481 cgccgtgccg cccagggcga gcgggtagac ccgcacgagg gcgccgcgga cccggtcaag
     3541 gccgagggcc agctgggcgg tccagcccga aatctcctgg cccagcgtga gcggcgtggc
     3601 gtccatcaag tgcgtgcgcc cgatcttgat gatgtcccgg tactcctcgg acttctgccg
     3661 cagcgtgtcg tgcagttgcg ccaccatcgg gatcagccgg cggtgggtct cctcggcggc
     3721 ggcgatgtgc atggcggtcg gaaacgtgtc gttggaggac tgggccttgt tgacgtcgtc
     3781 gttcgggtga atcggcttct tgctgccgag aacaccgccg gccagttcga tcgcccggtt
     3841 ggagatcacc tcgttggcgt tcatgttggt ctgggtgccg gagcccgtct gccagatcac
     3901 cagcgggaag tgctcgtcga gcttcccctc gattacctca tccgcggccc ggacgatcag
     3961 ctcccctttg tctttgggca gcgtgccgag ctccatgttg accagggcgg cggctttctt
     4021 gaggatcccc atggcgcgga tcagttcgcg cggcatgcgc tcgccgccga tgcggaagtt
     4081 gtgcagggac cgggcggtct ggcagccgta gtagcggtcg gccggcacct tcatctcgcc
     4141 catcgtatcg gtttcgatgc ggtattccat agagcctccc aaaggcgccc gcgggcgcag
     4201 ggttgtgatg tgattcgcag tgtccggagg aagtatacgc aattcgcgcc gcggggcaag
     4261 cggggggccg ggtgcggggc ttctccggcc gggggcgcca aagagaaggg ccgggtcact
     4321 tgcgtaaccc ggcccgaacc tttaagcgcc cgctctacca gcgttcgcgg tcgccgcgat
     4381 cgcggtcgcg tccgcccccg cgcccgccgc cgcccccgcc gccttcgcgc ggacgggctt
     4441 cgctgaccgt cagggcgcgg ccgccgtagt cgctcccctg gaggccgtca atcgccgcct
     4501 gggcctcttc gtcattcggc atttcgacaa acgcaaaccc gcgggaccga ccgctcaccc
     4561 ggtccatgat gatcttggcc gaggtcacgg tgccgtacgc ctcaaacttc gccttcagct
     4621 cctcttcatc agtgctgaac ggaagattgc caacgtagat attcatcgca tcaaactcca
     4681 aaaaaagact agttgccggt tttcatgttc ccccgctgcg cgcacatcag gtcatagggc
     4741 actcctccca agcgacaccg ttaaccgttg atcgggtctg cagcggggac aggcgacgtg
     4801 cggaggcgga ctgcagaccg gggccaccat gtatctcacc ttttgcacgg tcacggggtc
     4861 tccttgtcgg cgccgatgat ccgggccagg atgcgcgaca ccggcaccgc gccgtccgcc
     4921 agctcccgct tcatctgcgg gaacaggagc acctcgcgga tcgtgtgctg gttggtcaag
     4981 agcatgacga agcggtcgac gcccaggccg agcccggccg tcggcggcat gccgtaggtg
     5041 agggcggtga tgaaatcgtc atcgagcggc tgcgcctctt cgtcgccggc ggccagcgct
     5101 tttccctgct cgatgaaccg ccggagctgg tcgaccgggt cgttgagttc ggagaaggcg
     5161 ttggcggtct cctggccggc gatgaacaac tcgaaccgtt cggccaaacg gggattctcc
     5221 cggtggacct tggccagcgg cgaaatctcc cgcgggaaat cggcgatgaa ggtcggctgc
     5281 acgagcgtcg gttccacttt tgcttcccag atcgcctcca cgcacttccc ccagttggtc
     5341 aggtcctcgt cgggaaggtg catcttcttc gcctcggctc tcgcctgctc ataagacagg
     5401 gccatcaggt cgaggccggc cgcctcccgg atcgaatcca ccatcgacac ccaccggaac
     5461 ggcggggcga cgtcgatctc ccgctcgccg taggtgaagc ggtaggcgcc gtggacggca
     5521 aaggccacgt ggcgcaccag ctcttcgacc aggcgggcca tgtcgcggta gtccgcatac
     5581 gcccagtagg cctcgaccat cgagaactcc gggttgtgca gccggtccat cccttcgttg
     5641 cggaagtcct tgcagtactc ccagactttc tcgaatccgc cgacaatgag gcgcttgagg
     5701 tagagttcgt cggcgatccg gaggtacatg tcctcgtcga gcttgtggtg gtgcgtcttg
     5761 aacggccggg cgctggcgcc tccgtaaatc ggctgaagga tgggggtctc gacctcgagg
     5821 aagccgcggc ggttgaagaa gtcgcggatg gcctggacga tggcggtgcg cttgagaaag
     5881 gtcgcgcgca cctccgggtt ggcgatcagg tcggcgtagc ggcgccggta gcgcatttcg
     5941 acgtcggtca ggcccgcccg cttgtccgga agggggtgga gcgccttggt cagcagttcg
     6001 aaccggcgca cgttgagcgt ccgctcgccc gtgcgggtca cgaacagcgt cccctcgcaa
     6061 cccacgatgt cgccgaggtc gaccgcgtcg aacaggcgga actggtcctc gccgacggtg
     6121 tcgatcttga ggtagatctg gatgcggtcg gactgatcgc ggagatcggc gaaaaacacc
     6181 ttgcccatct tgcggatgag catgacgcgg ccggcgatcc gcaccgccgt ctggcgctcc
     6241 gcgagcgtgt cgaaatcggc gagcagggcg gcgatctcat ggtcgcggtc gtagcggtac
     6301 gggtaggggt tgatcccccg ggcctgaagg tcgcaggcct tctggtaccg gatgcgaatc
     6361 aggtcggcca gcgggatctg ggcgtcggac ggctccgact gcgggtgcgg ctccggggcc
     6421 ggatgggagt tcctatcagt caaagcggtt ccctgtcctg ggtggtatgg ttgtcatcac
     6481 gaacatagcg gatttccggc ggcgcccgtc gggttcgctc acgaacggag cttgttcctt
     6541 cgccgcagcg cggcgcggac cggcggcggc acgaggccgt tgacgtcgcc gccgtgaacc
     6601 gccacgtctt tcacaatggt cgacgagaga tagacccagg agagggccgg catgaggaac
     6661 acggtttcga tctcccgggc gagcttacgg ttcatgagcg ccatttggaa ttcgtactca
     6721 aagtcggaca ccgcccgcag gccgcggata atcgcgcggg ccttgacgtc ggtcgctacg
     6781 tcggccagaa gaccgtcgaa accgacgact ttcacttgag gggaaaaatc tctcgcgcga
     6841 acggattgct cgaccagctg aatgcgttcc tcaaactcaa aaaaaggatt tttcccggcg
     6901 ttcagggcca ccgcgacgat cacttcgtcg aacaggctgc aggcgcgttc gatcaacgac
     6961 atgtggccgt tggtgatagg atcaaacgta ccgggataaa cagctctgct tttggtcttc
     7021 gtcttcgtca ctgtcctcag agcgtccttt cccggtcacg ccggatgaac gccgctccgg
     7081 ctagtatacg cccgcggagc gggttacgca aggaaattag cgcccagccc catcgccgcc
     7141 agataccgtt ccggctgctc caattccccg cgcaaaggtc ccgccaaaaa cccggcgttc
     7201 accccgggcc tctcgatcgg aaccgtaaac tgcttctgca aagccgcttg gagagcctct
     7261 tcggccgctc cgccggaaac caccagggcc gaggggggaa gagtcaggcc gtagtcgaac
     7321 agggccgcca gacggaaact gaccaccgtc ttgatctcca ccgcgtaccg ctcgcgctcg
     7381 cccacgtgcc gaccgggc
//
LOCUS       DAOPOE010000131         7267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_11383_length_7267_cov_5.270937, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000131 DAOPOE010000000
VERSION     DAOPOE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11383_length_7267_cov_5.270937"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKM94_12790"
     CDS             <1..301
                     /locus_tag="PKM94_12790"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD67552.1"
                     /translation="VAGTCALIKHTERKFELAKMGVSPKFQGRGVGRRLCRAAIARAA
                     QLGAETLILATSPRLEAANHLYRSLGFMPVDTSEFGPLPYSRHSIVMALDLTEPE"
     gene            516..989
                     /gene="msrA"
                     /locus_tag="PKM94_12795"
     CDS             516..989
                     /gene="msrA"
                     /locus_tag="PKM94_12795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOD67553.1"
                     /translation="MAKATFGAGCFWGVEAAFRQLKGVRATAVGYAGGHTVYPTYKQV
                     CSDTTGHAEVVQVEYDPTEVTYEDLLRVFWDCHDPTQVNRQGPDIGSQYRSVIFYETP
                     EQETAARKSREELEKSGRFERPIATSIEPISSFWKAEDYHQQYLEKRGLSTCRMK"
     gene            1348..1935
                     /locus_tag="PKM94_12800"
     CDS             1348..1935
                     /locus_tag="PKM94_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67554.1"
                     /translation="MRMVARQTVVRVAVAALVGILAYAGIAGAAVRKPAKTSVPVPQV
                     VTTPSQSAPVPSAAAEPTLRQEPAEAQVNWQVMAGGGGSGTAGALQFSATIGQPIVGW
                     TSATGVNVHAGFWQDFSSGQCCENRGNVDHKFSGMNPVSVADITYLIAYVFRGGPVPP
                     CPEEADLTADGDITVVDITYLIAYVFRGGPAPPAC"
     gene            2170..3555
                     /locus_tag="PKM94_12805"
     CDS             2170..3555
                     /locus_tag="PKM94_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD67555.1"
                     /translation="MRIVKVEEVINSSFLKPGSVIYSAGNAATPQVILGQLARDPAIR
                     DIDLYCVLPLGDRIAALYTEERCQGLTHRVIFNSRLTKEAVNNGWAKYHPMHLREIPR
                     TMMNVVKPNIVLCSVAGPDNGGYYSLGTTVEGTLGAIRSAQRQGGLIIAERNRKLPFV
                     VGTTLPEADIDFLVDSDYELPRSPFREPDDRARRIGEIIAALYIHDGSGTEPGSTLQY
                     GIGEVPEAVTDAVLRKGVRNLGIHTELLAGAMIRLIQSGAVSNRWKPRINFAVSSIFL
                     AESQAQYDWLHYNSSVQIRPSDYTNSVFQIAEQPRMVSVNSAIGVDLHGNIWADSLDA
                     RKIYSGVGGQADFIRGAQYSDGGVAIIALKSTTRAGISKIVDRSPSGITTTAIPADQV
                     VLVTEYGAFDPRGLSLGERAVGIAHLAAPEERDKLLKVIHDNPAFHKPDLTLHKGIPG
                     FTPYERAVRSL"
     gene            complement(3662..5179)
                     /locus_tag="PKM94_12810"
     CDS             complement(3662..5179)
                     /locus_tag="PKM94_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD67556.1"
                     /translation="MKKVSRMGIFLLLALAAAAPGRAADLAGKTSLGVRLPFVIPLFE
                     GKNFQYYEYPGQRPRNIQPFLLGWNFGLELKYGVSNRVMLGLTGNYFYTHDDSTTESD
                     AGNQFHTSERARAKLSALAFGLNAYWYFLPEARLQPYLLGGAGIDHWTLTSTVNEDSS
                     HNSTDLNLKIGAGLMVPLNDQFAVDLQARYSHNVVVVSEDFPIGFYGQDTWGSYDDRP
                     FRSYLELTVGLACLFGGEPDTDGDGVKDSKDLCPGTPLGVVIDKAGCPLDGDADGVPD
                     YLDQCPDTPAGVAVDDKGCPRDTDGDGVADYLDKCADTPAGFKVDADGCPLDADGDGV
                     VDENDKCPDTPEGAPVDAAGCPLDSDKDGVFDYLDKCPGTPEAIPVDETGCPKLIKKG
                     EKITLHINFPTNSFEIDEASKRTLDGVAQTMLSFPDIKIRAGGFTDNTGSTGHNQRLS
                     ENRAKAVTAYLESKGVPADRMSAKGFGEDPKYFVGDNATEEGKALNRRVELESVE"
     gene            complement(5247..5936)
                     /gene="aqpZ"
                     /locus_tag="PKM94_12815"
     CDS             complement(5247..5936)
                     /gene="aqpZ"
                     /locus_tag="PKM94_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOD67557.1"
                     /translation="MKQYGAEFIGTFWLVLGGCGSAVLAAAFPGLGIGLLGVALAFGL
                     TVLTMAFAIGHISGCHLNPAVSIGLWAGGRFPAGKLLPYIVAQLLGGLLAGGILYLIA
                     SGKAGFDVAAGFAANGYGEHSPGGYALPAALVSEVVMTAMFLVVILGATDKRAPQGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALYVGGWAVQQLWLFWVAPIAGALVGA
                     VCYRLIDRSHT"
     gene            6492..>7267
                     /locus_tag="PKM94_12820"
     CDS             6492..>7267
                     /locus_tag="PKM94_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67558.1"
                     /translation="MKHSHLLRLAGFTLLVAVLIGPAAADWVPADGHKMHYPQLPDEA
                     GWDVNATQPLVLADDWQCSETGWVKDIHFWGSWKHGIEGQIVSFVLSIHADIPASQSP
                     TGYSMPGETLWEREVTQWDAQPLDPPSMEGWYDPLTGEVLYNDHQAYFQYNVFLPEAD
                     WFWQEAGTIYWLNVSAIVADPTGTTWGWKSTQDHWNDDAVWATWGNLNWIDMWEPAMP
                     KMNQFWITVDPAGQLVTGGGVGAYGQGWYYYPQIDWYNIW"
BASE COUNT         1317 a         2151 c         2537 g         1262 t
ORIGIN      
        1 cgtcgcggga acctgcgccc tcatcaagca cacggagcgc aagttcgagc tggccaagat
       61 gggggtgtcg ccgaaattcc agggccgggg ggtgggccgg cggctgtgcc gggcggcgat
      121 cgcccgcgcc gctcaactgg gggccgagac gctgatcctg gcgaccagcc cgcgcctgga
      181 ggcggccaac cacctctatc gatcgctcgg tttcatgccc gtggatacgt cggagtttgg
      241 accgcttccc tacagccggc attcgatcgt gatggcgctc gacctgaccg agccggagtg
      301 attgtggggg gggaagaaag cggggccgcg gaaatggcgc cccgaaagca gaggcaaccg
      361 tcgtgaagac tcataggacc gtattcattg gtgtactcct gaccgccgca ctgtcggccg
      421 ggtccgtctc gtgggcggca caagagccac cgacagggga tgcgagcggc tcggcggctg
      481 cggcgaaatc gacggattcc acggagaaag gcgatatggc gaaagcaaca ttcggtgcgg
      541 gttgtttctg gggcgtggag gcagcctttc ggcagctcaa gggggtcaga gcgacggccg
      601 tcggatacgc cggcgggcat accgtctacc cgacctacaa gcaggtctgc tcggacacga
      661 ccgggcacgc cgaggtggtt caggtcgagt atgatccgac cgaagtaaca tatgaggact
      721 tgctgagggt gttctgggac tgccacgatc cgactcaggt gaaccgacag ggtccggata
      781 tcggcagtca gtaccgctca gtgattttct acgagacgcc ggagcaggag acggcggccc
      841 gcaagtccag ggaggaattg gagaaaagcg gccggtttga gcggcctatt gcgacttcca
      901 ttgaaccgat ttcatcgttt tggaaggctg aggattacca ccagcagtat ctggagaagc
      961 gcgggctgtc gacatgccgc atgaagtgaa gccgggtggg cgcggtcggc ccaaaggatc
     1021 gcccggcggc tgcacccaag acatacgagg gatttgagac aagaagactc gggattcatc
     1081 cgaggacgcc ggcggaatgc gcgcgacggg ttgccgcgcg actgaggtta tcgacggcgg
     1141 caagatttcg gttgcccttt tccggaaagt gcgcatattg tcaaggagtt cccgattcgg
     1201 aagaccctcg gtacggcagc ccgttcggga aagacgcagg acgatcggct gacatgacat
     1261 accgacacgg aatcgagctt ccggtcctgc tcaatgatca cttggattac ttgcgaacgt
     1321 gacagatcgc tgatagagga gtgcgcaatg agaatggtcg ctcgtcaaac ggtggtgagg
     1381 gttgcggttg cggcgctggt cggcattctg gcctacgccg gcatcgcggg ggccgcggtg
     1441 cggaaaccgg cgaagaccag tgtgccggtg cctcaggttg tcacgacgcc gagtcagagc
     1501 gctccggtgc cgagtgcggc ggcggagccg accttgcggc aggagcccgc ggaggctcag
     1561 gtcaactggc aggtgatggc aggcgggggc ggcagcggca cggccggagc gctgcagttc
     1621 tcggccacga tcggccagcc gatcgtaggc tggaccagcg ccaccggggt taatgtccac
     1681 gccgggttct ggcaggattt cagttccggt cagtgctgtg agaaccgggg gaatgtcgac
     1741 cacaaattca gcggtatgaa tccggtctcg gtggcggaca tcacttacct gatcgcctac
     1801 gtcttccgcg gcggaccggt gccgccgtgc ccggaggaag cggatttgac ggccgacggc
     1861 gacatcacgg tggttgacat cacgtatctg attgcgtacg tcttccgcgg cggaccggcg
     1921 ccgccggcct gctgagcggc gcagggcggg cgggttcgac tgcgacaggg tggccacggt
     1981 cgggacaaag cagcccggcg gcgacccatc gagcgagagc gcgctctcgt tccgacctta
     2041 ttttcgccga cagatagaaa agggtggcgg cccgggcagg cttgtgttat ttttcacaag
     2101 cccgccgccg gtatgccccg gggccgggat taagtatgaa cgtgcagaag aaggtgttgg
     2161 caggtctgta tgcgaatcgt gaaagttgaa gaagtcatca actccagttt tctcaaaccc
     2221 ggaagcgtga tctactcagc cggcaacgcg gccacgccgc aggttatcct cgggcagttg
     2281 gcccgcgatc cggcgatccg ggatatcgat ctctactgcg tgcttcccct gggcgaccgg
     2341 atcgcggcgc tctacacgga ggaacgatgc caggggttga cgcaccgggt gattttcaac
     2401 agccggctga ccaaggaggc ggtcaataac ggctgggcga agtatcatcc gatgcacctg
     2461 cgggagattc cgcgcaccat gatgaacgtc gtgaagccga acatcgtgct ctgctcggtg
     2521 gcggggccgg acaacggcgg ctactacagt ctggggacaa ccgtggaggg gacgctgggg
     2581 gcgattcggt cggctcagcg ccagggcggg ctgatcatcg ccgagcgcaa ccgcaagttg
     2641 ccgtttgtgg tggggacaac gctgccggaa gcggatatcg atttcctcgt cgattcggat
     2701 tacgagctgc cgcgcagccc gttccgcgag cccgatgacc gggcgcggcg gatcggcgag
     2761 atcatcgccg cgctctatat tcacgacggc tccgggacgg aaccggggtc gacgctccag
     2821 tacggcatcg gcgaagtgcc ggaggccgtg accgacgccg tgctgcgcaa aggcgtccgg
     2881 aacctgggga tccacacgga gctgctcgcg ggagcgatga tccggctgat ccagtcggga
     2941 gcggtgagca accgctggaa gccgcggatc aatttcgcgg tgtcgtcgat cttcctcgcc
     3001 gagagccagg cgcagtatga ctggctccac tacaacagtt cggtgcagat ccggccgagc
     3061 gattacacca acagcgtctt ccagatcgcc gagcagccgc ggatggtgtc ggtcaacagc
     3121 gcgatcggcg tcgacctcca cgggaacatc tgggcggact cgctggacgc gcgcaagatt
     3181 tacagcgggg tcggcgggca ggccgacttt atccgcgggg ctcagtactc agacggaggg
     3241 gtggcgatca tagcgctgaa gtcgacgacg cgggcgggca tctccaagat cgtcgaccgc
     3301 tccccttcgg ggatcaccac gacggcgatc ccggcggacc aggtggtgct ggtcaccgag
     3361 tacggggcgt tcgatccgcg cgggctgtcg ctcggggagc gggcggtcgg gatcgcccac
     3421 ctggcggcgc cggaggagcg ggacaagctg ctcaaggtga ttcacgacaa cccggccttt
     3481 cacaagcccg atctcacgct gcacaagggc attcccgggt tcacgccgta cgagcgggcg
     3541 gtgcggtcgc tctaggccgc ctgggtaagc ggcgggcggc gtcgtccccc gcgatggggc
     3601 caagaaatac ggaggcggcc gttcggccgc ctccgcgcat tccaaaaccg accggtcgtg
     3661 ctcactcgac tgattcgagt tccacccgcc ggttgagggc tttcccttcc tcggtggcgt
     3721 tgtcgccgac gaagtacttg ggatcctcgc cgaaaccctt cgctgacatc cgatccgccg
     3781 gtaccccctt gctctcgagg taggcggtga ccgccttggc gcggttctcc gacagccgct
     3841 ggttgtgccc ggtgctgccg gtgttgtcgg tgaagccgcc cgcgcggatc ttgatatccg
     3901 ggaacgagag catcgtctgc gccaccccgt cgagagtcct cttcgacgcc tcgtcgatct
     3961 cgaacgagtt ggtggggaag ttgatgtgga gagtgatctt ctctcccttc ttgatcagct
     4021 tcggacaccc cgtttcatcg accggaatcg cctcgggcgt gcccgggcac ttgtcgaggt
     4081 agtcgaacac gccgtccttg tcggaatcga gcggacagcc cgcggcgtcc acgggagcgc
     4141 cctcaggggt gtcggggcac ttgtcgttct cgtcgaccac cccgtcgccg tcggcatcca
     4201 gcggacagcc gtcagcgtcg accttgaagc cggcgggagt gtcggcgcac ttgtcgaggt
     4261 agtccgccac gccgtcgccg tcggtgtcgc gcgggcaccc cttgtcgtcg accgccacgc
     4321 cggcgggcgt gtcggggcac tggtcgaggt agtcgggcac gccgtccgcg tcgccgtcga
     4381 gcggacagcc cgccttgtcg atcaccacgc cgagcggagt gccggggcag agatccttcg
     4441 agtccttcac cccgtcgccg tcggtgtcgg gttcgccgcc gaacaggcag gcgagtccga
     4501 ccgtgagctc caggtagctg cggaacggcc ggtcatcgta gcttccccag gtgtcctgac
     4561 cgtagaaccc gatcgggaag tcctcggaga cgacgacgac gttgtgggag tacctggcct
     4621 ggaggtcgac ggcgaactgg tcgttgagcg ggaccatgag gccggcgccg atcttgaggt
     4681 tgagatccgt cgaattgtgc gacgagtcct cgttgacggt ggatgtgagg gtccagtggt
     4741 cgatgccggc gccgccgagg aggtacggct ggagccgggc ctcggggagg aagtaccagt
     4801 aggcgttgag cccgaaggcc agcgcgctca gcttggcccg ggcgcgctcg ctggtgtgga
     4861 actggttgcc cgcgtcgctc tcggtcgtcg aatcgtcgtg ggtatagaag tagttgccgg
     4921 tgagtccgag catgacgcgg ttggagacac cgtacttcag ttcgagcccg aaattccagc
     4981 ccaacaggaa cggctggatg tttctcggcc gctggccggg gtactcatag tactggaagt
     5041 tcttcccctc gaacagcggg atgacgaacg ggagcctgac tcctaggctg gttttgcccg
     5101 cgaggtcggc ggctcgcccg ggggcggccg cggcgagcgc cagcaggagg aagatcccca
     5161 ttcgtgagac tttcttcatc atgtgttcca tcctttgttt ggtgagtgct gcctcgtgtg
     5221 tgtgtgcggc ccggccgggc cggccgctag gtatgactcc tgtcgatgag acggtagcag
     5281 acggctccga ccagggcgcc ggcgatcggg gctacccaga agagccagag ctgttgcacg
     5341 gcccagccgc cgacatagag ggccacgccc gtgctgcggg cggggttgac cgaggtgttg
     5401 gtgacaggaa tgctgatcag gtgaatcagg gtgagggcca ggccgatcgc gatcggagcg
     5461 aacccctggg gggcgcgttt gtcggtggcg ccgaggatga cgacgaggaa catggcggtc
     5521 atgacaactt cgctcaccag cgccgccggc aacgcatagc cgcccggcga gtgctccccg
     5581 tagccgttgg cggcgaaccc ggccgccacg tcgaaaccgg ctttgccgct ggcgatgaga
     5641 tagaggatgc cgccggcgag gagcccgccc aggagctggg ccacgatata cggcaagagc
     5701 ttgccggcgg ggaatcgtcc gccggcccaa aggccgatcg agacagccgg gttgaggtgg
     5761 cagcccgaaa tgtggccgat tgcgaacgcc atcgtcagca ctgtcagtcc gaaggccagg
     5821 gcgacgccga gaaggccgat acccaggccg ggaaaggcgg ccgccagcac ggcgctaccg
     5881 catcccccga gaaccagcca gaacgttccg atgaactccg caccatactg cttcatgagc
     5941 catctccttt cagaagacac ggggttacga gggtcgcgcc gctcgcgccg gttcgccgcg
     6001 ccgcagcatc ccgacgaagc tcagaggatc atttgcatac accgccatac gctgctcccg
     6061 ctcgtcgtgg atcggcccat cattctttcc cggtttcacg ataggggttg gggtaggccg
     6121 acgcaaacat aaaatcagcg cccggaatgc gggggccggc ggcgccgggg gcggaagatg
     6181 atagtcaaca tggactcgca cggcgggtta cgcgcaccgc ggacagtgcg cccggtcccc
     6241 ggaggcgacc tccggccggg tgaaggatcc atctcctgtc ccggcgagcg aattagacct
     6301 tgacaggtgc tgtcagaacc cgttaattaa agcggatacg catggctgtt gcttaaagtg
     6361 acatcgtcga acgacgccgt cgagcagaac ttccacgcgc ggcttggcat cgtatccgct
     6421 gaggtcggtc agcaggaatc gctatatcgg caaaactgaa tcgtacagtc acgaatgtca
     6481 gtggaggaag catgaagcat tcgcacttgc tccgtctggc gggtttcact cttttggtgg
     6541 cggtcttgat cgggccggcc gccgccgatt gggtgccggc cgacgggcac aagatgcact
     6601 atccccagct tccggatgag gcgggatggg atgtgaacgc cacacagccg ctcgttctgg
     6661 ccgatgactg gcagtgcagc gagacgggct gggtgaagga catccacttc tggggctcat
     6721 ggaagcacgg gatcgagggg caaattgtat cgttcgtcct gagcatccac gcggatattc
     6781 cggcgagcca gagtccgacc gggtacagca tgccgggaga gaccctgtgg gaacgggagg
     6841 tgacgcagtg ggatgcccag ccgttagacc cgccgtcgat ggagggatgg tacgatccct
     6901 tgaccggcga ggtgctctac aacgaccacc aggcctactt ccagtacaac gtgtttctgc
     6961 ccgaggcgga ctggttctgg caggaggcgg ggacgattta ctggctgaac gtgtcggcca
     7021 tcgtcgccga tccgaccggc acgacctggg ggtggaagtc gacgcaggat cactggaatg
     7081 acgacgccgt gtgggcgacc tgggggaacc tgaactggat tgacatgtgg gagccggcca
     7141 tgccgaagat gaaccagttc tggatcacgg tggatccggc ggggcaactg gtgaccggcg
     7201 gcggcgtcgg ggcctacggc caggggtggt actactaccc gcagatcgac tggtacaaca
     7261 tctggtt
//
LOCUS       DAOPOE010000132         6951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12012_length_6951_cov_5.608324, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000132 DAOPOE010000000
VERSION     DAOPOE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12012_length_6951_cov_5.608324"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..742
                     /locus_tag="PKM94_12825"
     CDS             143..742
                     /locus_tag="PKM94_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD67559.1"
                     /translation="MNFSRPAARPLTARLPVLLLALALSAAPAGVSARIVYGSLQAGL
                     SIPAQGNRLNDRFDRGFGLGGGAVFVLGEEVNMIVQIEYHSLRNAGLPPLADRGSLRA
                     AMLTTMFRYMFGQDLDPWRPFLGLGFGTADLSLAKEGDTYTEWDGDDLHGVFHVGAGL
                     QVRVLDRFWIIAQARWMTISSPGGGDASTLYPISLGLEF"
     gene            complement(840..1346)
                     /locus_tag="PKM94_12830"
     CDS             complement(840..1346)
                     /locus_tag="PKM94_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67560.1"
                     /translation="MNSFEFLFDGAVTEARLEREGGDFHVTVGDTNLRLHPAGRNLYD
                     VLVNGRRMLVAAVRHKDKFYIDIDSVLLEVREATEENVAGAAGDHAVDPDKIFAPMPG
                     KIVKILVEVGQTVAEKQQMVIVEAMKMENPVLARASGTVKAINYRDGEQVGTEAPIIE
                     LELAKKGE"
     gene            complement(1343..2857)
                     /locus_tag="PKM94_12835"
     CDS             complement(1343..2857)
                     /locus_tag="PKM94_12835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOD67561.1"
                     /translation="MKQPFKKILVANRSEIAVRVMNACRLLDIPCVAVYSDADANAKH
                     RWYADEAVHIGAPPPRESYLAMEKIIVAALHTGCDAIHPGYGFLAENPVFAQKCGEAG
                     LVFIGPSPEAIRLLGNKVESRIRMASAGVALIPGMKKSDASTDEFVEAAAAAGYPVII
                     KAAAGGGGKGMRVVHRPEDLKDAIEAARREAGNAFGDDTVYLEKYIVNPRHIEFQVLG
                     DRHGTTVHVFERECSIQRRHQKIIEETPSTALDDELRGRMGAAAVKVAQAAAYTNAGT
                     VEFLLDRDRNFYFLEMNTRIQVEHPVTEMVTGVDLVVEQIRIAAGEPLAEPFRRLTQR
                     GHALECRIYAEDGEANFMPSTGTIVHYSEPAGPGIRVDSGVVEGTEITIDYDPIMAKL
                     IVHAPTRELAVRKMVRALNEYKLLGVKTSKRFMIDVLQHPEFEAGRTYTNFIEAHMQE
                     RSIDTALYRDPAIAAAAAAAAGAAGESRTAARGKREMPSAAELIGAWEIGGSIR"
     gene            complement(2907..3689)
                     /locus_tag="PKM94_12840"
     CDS             complement(2907..3689)
                     /locus_tag="PKM94_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD67562.1"
                     /translation="MKLETVTFETHERLARVTFNRPEIHNAFNATVIYEMTEVFRRIA
                     ADDAVRAVVLSGRGKSFCAGADLNWMRGVIRQTFEQNLAEATALAELFWLIYTCKRPV
                     IGRINGAAIGGGTGFVAVCDIALAARSAVFSFSEVKIGVVPACIGPYVIKKMGEGKTR
                     ELFITGERMSAERARAVGLVNGVADDDKLDEETDRLVHAILTSGPEAVAMAKRLISEA
                     PGMTPEQFKPYTAEMIARLRVSAEGQEGMDAFLNKRKPRWAE"
     gene            complement(3699..5306)
                     /locus_tag="PKM94_12845"
     CDS             complement(3699..5306)
                     /locus_tag="PKM94_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD67563.1"
                     /translation="MFRIESKIDTRTETFAKNDELNRAAHRLFKERLEQVKRGGPEKA
                     RARHTERGKLLPRDRVARLLDRNTPFLELSPLAAYDMYDDDAPAAGIITGIGTVHGRE
                     VMVIANDATVKGGTYYPITILKHARAQRIAEENHLPCVYLVDSGGIFLPLQSGTFPDK
                     DHFGRIFYNQARMSAKGITQISVVMGSCTAGGAYLPAMSDETIMVRKQGTIFIGGPPL
                     VKAATGEVVTAEELGGADVHCRTSGVSDHYAMNDEHAIQIARNIVQNLNRLEKFPIDW
                     AEPEDPYYDPEELYGVVSHDIRKPFDVKEVIARIVDGSRFHEFKELYGTTLVTGFARI
                     MGYPVGILGNNGVLFSESSLKGAHFIELCTERKIPLVFLQNISGFIVGRQYEAGGIAR
                     DGAKLVHAVANADVPKFTVVIGGSYGAGNYAMCGRGYFPRLMWMWPNAKICVMGGEQA
                     ADVLATVKIKQLESEGRKLSAEEIEAIRRPIIENYESQSTPYYSGARLWDDGMIGFGE
                     TRQALALGISMALNTAIPDQKYGVFRM"
     gene            complement(5329..>6951)
                     /locus_tag="PKM94_12850"
     CDS             complement(5329..>6951)
                     /locus_tag="PKM94_12850"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HOD67564.1"
                     /translation="YAVDFLEQLQDCLPLICRKNVRVIASAGGINPIGLGRKIVEMAR
                     GMGLELKVGIVYGDDIVNQLYELTAAGEKFANLETGEDFFAYRSRITSANIYLGAEPV
                     VKALEAGSQIIVTGRVTDTGITLAPMIHEFGWAMDDWDKMAAGIVAGHIIECGAQASG
                     GNITDWREIKSFHNIGYPIIEMEAGGEFAVTKHPKTGGLISEKTIKEQLVYEMGDPAN
                     YISPDGVARFDTIALRQVGPDRVRVSEVKGKPAPDHLKVSMSYDDGWKASGSVLVSGP
                     QAREKAGVIADTLWAKLDHAYEATRTEVIGTGSIWPRAFATDETNEVLLRFSVRDRDY
                     EKVRDFGKALPTLILSGPPGMAVTTGGRPKPSSVVAYWPALIHRSRVKAKVLVVGTNR
                     QESFHEITFPVRVPSPAADTPAGPPRRAPGRKPAGRAVEVRLEEICYARSGDKGDTCN
                     VGVLARSPEIYDWIAEHLTAARVQEFFAGITRGRVKRYELDNLGGLNFLLEETLGGGG
                     TRSLMIDPQGKTLAQALLQMRLRVPQSLLKTL"
BASE COUNT         1055 a         2512 c         2051 g         1333 t
ORIGIN      
        1 cacaaatttc ccccgccggc tcactcgaac cgcctccctc ccccgcccgg ccgcccgtct
       61 ctgcccgtcc ggacatttcg tttgtcccgt gtccccgtcc gggctatttt gttccggcaa
      121 tcgcttctcg aaaggagacc ggatgaactt ctcccgccct gcggcccggc cgctgacggc
      181 tcgcctgccg gtcctgctgc tggctctggc cctgagcgcc gccccggccg gcgtctccgc
      241 gcgaatcgtc tacggctcgc tccaggccgg tctgtcgata cccgcccagg gcaaccgtct
      301 gaacgaccgc tttgaccgcg gattcggtct cggcggcggc gccgtgttcg tgctcggtga
      361 agaggtcaac atgatcgtcc agatcgaata ccacagcctc cgcaacgccg gccttccccc
      421 gctggccgac cgcggctcgc tgcgcgccgc catgctcacc acgatgttcc ggtacatgtt
      481 cgggcaggat ctggacccct ggcggccgtt cctcggcctt ggtttcggca ccgccgacct
      541 atcgctggcc aaagaagggg acacctatac ggagtgggac ggcgacgatc tccacggcgt
      601 gtttcacgtc ggcgccggcc tccaggtgcg cgtgctcgat cgtttttgga ttatcgccca
      661 ggcgcgctgg atgaccattt cctcgcctgg gggcggcgac gcgagcactc tctatcctat
      721 ttcgctcggg ctcgagttct agggccggac ccggcccgcc cgcagagagt cggccggcgc
      781 gggcgccgct cagcccgaca gatgtcggtc gggccgctgc gcccgcggcg ttgattccct
      841 cactcgccct tcttcgccaa ttccagctcg ataatcgggg cctcagtccc cacctgctcc
      901 ccgtcgcggt agttgatcgc cttgactgtc ccgctcgccc gcgcgagcac cgggttttcc
      961 attttcatcg cctcgacgat caccatctgc tgcttctcgg cgaccgtctg ccccacctcg
     1021 accaggatct tgacgatctt ccccggcatg ggcgcgaaga ttttgtccgg atcgacggcg
     1081 tggtcgcccg ccgccccggc gacattttcc tcggtcgcct cccgcacctc caggaggacc
     1141 gagtcgatat cgatatagaa cttgtccttg tgccgcaccg cggccaccag catccgccgt
     1201 ccgttgacga ggacatcgta gaggttccgg ccggccgggt ggaggcggag gttcgtgtcg
     1261 ccgaccgtca cgtggaaatc tcccccctct ctttccagcc gggcctcggt gacagcgccg
     1321 tcgaagagaa actcgaagct gttcatcgga tggaacctcc gatctcccag gccccgatca
     1381 actcggccgc ggacggcatt tctcttttcc cccgggccgc tgtgcgcgat tcgcccgccg
     1441 ctccggccgc ggcggcagcc gccgccgcga tcgctggatc gcgatacaag gcggtgtcga
     1501 tggaccgttc ctgcatgtgc gcctcgatga aattcgtgta ggttcggccg gcctcgaatt
     1561 ccgggtgctg cagtacgtcg atcatgaacc gcttcgaggt cttcaccccc agcaatttgt
     1621 actcgttcag cgcgcgcacc atttttcgga ccgccagttc ccgggtcggc gcgtggacaa
     1681 tcagtttcgc catgatcggg tcgtagtcga tcgtgatctc cgtcccctcg accacccccg
     1741 aatcgaccct gatccccggc ccggccggct ctgagtagtg gacgatcgtc ccggtcgagg
     1801 gcatgaagtt cgcctcgccg tcctccgcgt agatgcggca ctcgagcgcg tgcccgcgct
     1861 gggtgagccg gcggaagggt tcggccaacg gttcccccgc cgcgatccgg atctgctcca
     1921 cgaccaggtc gacgccggtc accatctcgg tcaccgggtg ttccacctgg atgcgggtgt
     1981 tcatctccag gaagtagaaa ttccgatccc ggtcgagcag gaactccacc gtcccggcgt
     2041 tggtgtaggc ggccgcctgg gccactttga cggccgccgc tcccatccgc ccgcgtagct
     2101 catcgtcgag cgcggtcgag ggcgtctcct cgatgatctt ctggtgccgc cgctggatcg
     2161 aacactcccg ctcgaacacg tgcacagtgg tcccgtggcg gtcgccgagc acctggaact
     2221 cgatgtggcg ggggttgacg atgtacttct cgaggtagac ggtatcgtcg ccgaaagcgt
     2281 tgcccgcctc acggcgggcc gcctcgatcg catccttgag atcctccggg cggtgcacga
     2341 cccgcatccc cttgccgccc ccgccggcag ccgccttgat gatcaccggg tagccggccg
     2401 cggccgcagc ctccacgaac tcgtccgtgg aggcgtccga tttcttcatc ccgggaatca
     2461 gtgccacgcc cgccgacgcc atccggatgc gcgactcgac tttgtttccc agaagccgga
     2521 tcgcctcggg cgagggaccg ataaagacga ggcccgcctc gccgcacttc tgcgcaaaca
     2581 ccgggttctc ggccaaaaac ccgtaccccg gatggatcgc atcacacccg gtgtgaagcg
     2641 cggcgacgat gatcttctcc atcgccaggt aggactcccg cggcggcggc gcgccgatgt
     2701 gcaccgcctc atccgcgtac cagcggtgct tcgcgttggc gtcggcgtcg gagtagaccg
     2761 ccacgcacgg aatgtcgagg aggcggcagg cgttcatgac gcgcaccgca atctccgacc
     2821 ggttggcgac tagaatcttc ttgaatggct gcttcatgac tgcatatccc gtcgtctgtt
     2881 tagtgcgagc ttgccggccc gacccatcac tccgcccacc tgggcttacg cttgttcaaa
     2941 aacgcgtcca tcccttcctg cccctccgcc gagacccgga gccgcgcgat catctcggcc
     3001 gtgtacggct tgaactgctc cggggtcatg cccggcgctt cgctgatcag ccgtttggcc
     3061 atcgccaccg cctccggccc cgaagtcagg atcgcgtgga cgagccggtc cgtctcttcg
     3121 tcaagcttgt cgtcgtccgc caccccgttg accagcccca ccgcgcgggc ccgttcggcg
     3181 ctcatgcgct cgcccgtgat gaacagctcc cgcgtcttcc cctcccccat ctttttgatc
     3241 acgtacggcc cgatgcacgc gggcacgacg ccgatcttga cctcggagaa ggagaacacc
     3301 gccgaccggg cggcgagggc gatgtcgcac accgcgacaa acccggtgcc gcccccgatc
     3361 gccgccccgt tgatccgtcc gatcaccggc cgcttgcagg tgtagatgag ccagaacaac
     3421 tccgcgagcg cggtcgcctc ggccagattt tgctcgaacg tctgccggat cacgccgcgc
     3481 atccagttga gatcggcccc ggcgcagaac gatttgcccc ggccgctgag caccaccgcc
     3541 cgcacggcat cgtcggcggc aatcctccgg aacacctccg tcatctcata aatcaccgta
     3601 gcgttgaacg cgttgtggat ctcggggcgg ttgaacgtca cccgcgccag gcgctcgtga
     3661 gtttcgaatg tcacggtctc cagcttcatg ccgcctcctc acatcctgaa cacgccgtac
     3721 ttctggtcgg gaattgccgt gttgagcgcc atcgatatcc cgagcgcgag cgcctgccgg
     3781 gtctcgccga aaccgatcat cccgtcatcc cacagccgcg cccccgagta gtacggcgtc
     3841 gactggctct cgtagttttc gatgatcggc cggcggatgg cttcgatttc ctccgcgctc
     3901 agcttgcgtc cctcgctctc caactgcttg atcttgaccg tcgccagcac gtcggccgct
     3961 tgttcgcccc ccatcacgca aatcttcgca ttcggccaca tccacatgag ccgggggaaa
     4021 tacccccgcc cgcacatggc gtagttcccc gccccgtagg agcccccgat cacgaccgtg
     4081 aacttgggca catcggcatt ggccaccgcg tgcaccagct tcgcgccgtc gcgggcgatc
     4141 cccccggcct cgtactgccg cccgacaatg aaccccgaga tgttctggag gaacacgagc
     4201 gggatctttc gctccgtgca cagttcgata aagtgcgccc ccttgagcga ggactcggag
     4261 aagagcacgc cgttgttgcc gaggatcccc accgggtacc ccatgatccg tgcaaacccg
     4321 gtcacgagcg tcgtcccgta caattccttg aattcgtgga agcgcgatcc gtcgacgatc
     4381 cgcgcgatga cctccttgac gtcaaacggc ttgcggatgt cgtgcgacac cactccgtag
     4441 agctcttccg gatcgtagta gggatcctcc ggctccgccc agtcgatcgg aaacttctcc
     4501 aggcggttca ggttctgcac gatgttgcgg gcgatctgaa tggcgtgctc gtcgttcatc
     4561 gcgtagtggt ccgacacccc cgaggtccgg cagtggacgt ccgccccgcc gagctcctcg
     4621 gccgtgacca cctccccggt cgcggctttc accagcggcg gccccccgat gaagatcgtc
     4681 ccctgcttgc gcaccatgat agtctcgtcg gacatcgccg gcaggtatgc cccgcccgcc
     4741 gtgcacgacc ccatcaccac cgaaatctgc gtgatcccct tggccgacat gcgcgcctgg
     4801 ttgtagaaga ttcgcccaaa gtggtccttg tcgggaaacg tccccgactg cagggggagg
     4861 aatatcccgc cggaatcgac gaggtagacg cagggcaggt ggttttcctc ggcaatccgc
     4921 tgcgcccggg catgcttgag aatcgtgatc gggtagtacg ttcccccctt gaccgtcgcg
     4981 tcgttcgcga tcaccatcac ctcgcgtccg tgcacggtcc cgatccccgt gatgatgccg
     5041 gccgcaggcg cgtcgtcgtc gtacatgtcg taggccgcca gcgggctcag ttcgagaaac
     5101 ggggtgttgc ggtcgagcag ccgggccacc cggtcgcggg gaagcagctt cccgcgctcg
     5161 gtgtgccgcg cccgcgcctt ctccggcccg ccccgcttca cctgctccag tcgttccttg
     5221 aagaggcggt gcgccgcccg gttgagctcg tcgttcttgg cgaacgtctc cgtgcgcgtg
     5281 tcgatcttgg actcgattcg aaacattggc gtctgtcttt ccccaaggct agagtgtttt
     5341 caacagcgac tgcggcacgc gcagcctcat ctgaagcagc gcctgcgcga gcgtcttccc
     5401 ctgaggatcg atcatgagcg accgcgttcc gccgccgccg agcgtctcct cgagcaggaa
     5461 attgagcccg cccaggttgt ccagctcgta ccgcttcacc cggccgcggg tgattcctgc
     5521 gaagaattcc tggacccggg cggccgtcag gtgctcggcg atccagtcgt agatctcggg
     5581 cgaccgcgcg agcaccccga cattgcaggt atcccccttg tcccccgacc gggcgtagca
     5641 gatttcttcg agccggactt cgaccgcccg gccggccggt ttgcggccgg gcgcgcgtcg
     5701 gggcggcccg gcgggcgtgt ccgcggccgg cgacggcact cgcaccggaa aagtaatctc
     5761 gtggaacgac tcctgccgat tcgtcccgac caccagcact ttcgccttca ctcggctccg
     5821 gtgaatcagc gccggccagt aggcgaccac gctcgagggt ttcgggcgac cgccggtggt
     5881 cacggccatc cccggcggcc cggagaggat cagcgtcggc agcgcctttc caaaatcccg
     5941 cactttctcg tagtcccggt cgcggaccga aaaccgcagg agcacctcgt tggtctcgtc
     6001 ggtcgcgaac gcccgcggcc agatcgagcc cgtcccgatc acctccgtcc gcgtcgcctc
     6061 ataggcgtgg tccagtttcg cccacagggt gtcggcgatc accccggcct tctcgcgcgc
     6121 ctgcgggccc gacacgagga ccgacccgct cgccttccac ccgtcgtcat acgacatcga
     6181 aactttcaga tggtccggcg ccggtttccc cttgacctcc gacacccgca cccggtcggg
     6241 gccgacctgc cggagcgcaa tagtgtcgaa gcgcgccacg ccgtccggcg aaatgtagtt
     6301 ggcgggatcg cccatctcgt agaccaactg ctccttgatc gtcttctccg agatcaggcc
     6361 gccggttttg gggtgtttgg tgacggcaaa ttcgccgccc gcttccattt cgataatcgg
     6421 gtagccgata ttgtggaagc tcttgatttc gcgccagtcg gtgatatttc cccccgaggc
     6481 ctgcgcgccg cattcgataa tgtgcccggc gacaatcccc gccgccatct tgtcccagtc
     6541 gtccatggcc cagccgaact cgtgaatcat cggcgccagc gtaatcccgg tgtcggtcac
     6601 ccggccggtc acgatgatct ggctgccggc ctcgagcgct ttcaccaccg gctccgcgcc
     6661 gaggtagatg ttggccgagg tgatgcgcga acggtaggcg aagaaatcct cgcccgtttc
     6721 gagattggcg aacttctccc ccgccgcggt cagttcgtac aactggttga cgatgtcgtc
     6781 cccgtagaca atccccacct tgagttccag gcccatgccc cgcgccattt cgacgatttt
     6841 ccgccccagc ccgatcgggt tgatgccccc ggcgctggcg atgacccgca cgttcttacg
     6901 gcagatcagg ggaaggcaat cctggagctg ctcgagaaaa tccaccgcgt a
//
LOCUS       DAOPOE010000133         6789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12363_length_6789_cov_5.330264, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000133 DAOPOE010000000
VERSION     DAOPOE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12363_length_6789_cov_5.330264"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..418)
                     /locus_tag="PKM94_12855"
     CDS             complement(53..418)
                     /locus_tag="PKM94_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD67565.1"
                     /translation="MNINISPHDQFKALADPTRLRLLVLLTAGELCVGDLAATLGLPQ
                     PTVSRHLARLKLLGWVEDRRAGKRVHYRLAHPETAVGTAIAELLPRLAAEPPFGEDRQ
                     RLKEHCRGGDAGGERGAEI"
     gene            726..1418
                     /locus_tag="PKM94_12860"
     CDS             726..1418
                     /locus_tag="PKM94_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOD67566.1"
                     /translation="MRKVFTVALVLAVAMMFAGAAAYADGSCAVKKTAESASAKSACT
                     AGKTAEAKMTSAENAGCSGHKTGETAMTAAGSCANAKFMAGCDPSFCTPEDCAAWGKM
                     CQAHGENAEVRMMSVKGMTCTGCENSIKAALEKAPGVLEVVKVDHKRGLAVVIVDKTM
                     AKNDDMTTLVTNKGYEAQIIPAVATTGTAAPAAKVSDGAKKSCAATCAKPCDKAKSTE
                     TKAGDKSTSTPH"
     gene            1630..4077
                     /locus_tag="PKM94_12865"
     CDS             1630..4077
                     /locus_tag="PKM94_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67567.1"
                     /translation="MKYTFVSLALCLIFAGSILAEDPLPTQPRGKSIREYLTPDGRFD
                     LDAARRSGYEGPLDIEGFRSGIDPATRAPLFQPASPAFPADHPDDIFWDNSISPSIPG
                     VDSAVRAAIVYDGNLVVGGSFQAAGDVVASYIASWNGSAWSSLGSGMNNSVYALTVHN
                     GDLIAGGTFTTAGGASANRIASWDGSAWSSLGSGMNDYVLALTVYDGGLIAGGDFTMA
                     GGTSANRIASWDGSAWSSLGSGMSDWVHALTVYDGNLIAGGEFTMAGGTSAKGIASWN
                     GSAWSSLGSGMNGFVRALTVYDGSLIAAGYFTTAGGTSAGSIASWNGSVWSPLGSGIN
                     YEVRALTVYDGNLIAGGYFVVAGGTSAYCIASWNGSSWSPLGSGMNDYIGTLTVYDGD
                     LIAGGYFATAGGTNANFIASWNGSSWSSLGSGMDYGVYALTVYGGNLMAGGWFSTAAG
                     TSANYIASWNGSAWSSLDSGMNDYVEALTVYDGNLIAGGHFDTAGGTSANFIASWDGS
                     AWSSLGSGMGYYGYVNGLTVYDGNLIAGGYFTTAGGTSAGSIASWDGSAWSSLGSGMS
                     DYDSYVYALTVYDGNLIAGGYFTTAGGTTANHIASWDGSAWSSLGSGMNDAVEALTVY
                     DGNLIAGGEFTTAGGTSASYIASWDGSAWSSLGSGMNDAVEALTGYGGNLIAGGGFTT
                     AGGTSANRIASWNGSAWSSLGSGMNGYVLALTVCGGALIAGGDFAIAGNKVSGHLASW
                     TKCCNGTRGNVNFDAAGAVTVADITFLINHVFRGGPEPDCFEEADVTADGKLAVADIT
                     FLIAYVFRGGPAPAACP"
     gene            complement(4542..6170)
                     /locus_tag="PKM94_12870"
     CDS             complement(4542..6170)
                     /locus_tag="PKM94_12870"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD67568.1"
                     /translation="MCDSPKVRNSWIPAPGQPITTIAVFFHIITGDDGANPSLSADNL
                     AVAVAAMNADFLPYRIQFRYDYRFISSSQFRVLDSWDECNLMKETYALDPEYQCNIFV
                     TGVDIDGQSFSWGTYPWEPGAEGPRGGIFMASNHMPPDDDGTLTHEMGHALGLYHTFY
                     GVEEVEPCGVCYESPQQLTNDWVGDFCSDTDPTPENYYCLPPSGTDPCTDIAWGPTQP
                     QNHMSYAPLWCRYEFSRQQTGRMHCWLRDRLESWIVSVSFEADVASGPVPLDVQFTSE
                     TLEDVLSWSWSFGDGDSADVPHPSHTYTGPGRHDVSVTIVTAHGAFTRTRSDYIWAQA
                     DTLIVPGGLGRVEESVRFDIGARNTQPVDRIRIPFDWNGPLSMSLDSVSTAGLRTADI
                     ANHGFLHCDPSNRCATYEVNPPPGQEILPDTGALLSIYFTCSSGYHGQTNPIAILSYG
                     GYEPLLSCPQGPVVPILLPGELEICGTGDVNYNGDGPNIADLIYLVDYLFRGGPEPPS
                     AATANIDGLLPVNVVDLTSLVGYLFGTGPPPPCL"
BASE COUNT         1259 a         2102 c         2137 g         1291 t
ORIGIN      
        1 cggtcactga gcctgcccga accgcggggg agaagcggcg ggaaatgctc tgtcagatct
       61 ccgcaccgcg ctcgccgccg gcatcgccgc cccggcagtg ctccttcagc cgctgcctat
      121 cctccccgaa gggcggctcg gccgcgaggc gggggagaag ctccgcaatc gccgtcccca
      181 ccgccgtctc cgggtgggcc aggcggtagt gcacccgttt ccccgcccgg cggtcttcca
      241 cccagccgag cagtttcagc cgggcgaggt gccgcgacac ggtcggttgc ggcagcccca
      301 gagttgcagc cagatcgccc acgcacagct cccccgctgt cagcagcacg agcagccgca
      361 accgggtcgg gtcggccagg gctttgaact ggtcgtgcgg tgatatatta atattcatat
      421 atccgcatat aagtgaaatg accttgtgcg atcaaggttt ttcttccggt aaccgattga
      481 agataaagac gatggcaata agggactgaa atagtacgct tttttctgaa accatccccc
      541 ggtctggggt gttaaaccga cataccgata ggtgggatag ccgatgccga tgacgccaat
      601 acaacacatc atcgcccaaa aatatccaca aaccggtaat cgcgcttgac tggccggata
      661 aaaaaacgta tactgtacca taaaacctga accgctgatg tacccgtgtg aagaggagta
      721 acaccatgag aaaagtattc accgtcgccc tcgttctggc ggtcgccatg atgtttgccg
      781 gcgccgcagc ttatgccgat ggctcctgtg ccgtcaagaa gaccgcggaa tccgcctcgg
      841 ccaagtcggc ctgcacggcg ggcaagactg ccgaagccaa gatgacctcg gctgagaatg
      901 ccggctgctc cggccacaag accggggaga cggcgatgac cgcggccggc agttgcgcca
      961 acgccaagtt catggccggc tgcgatccgt cgttctgcac tcccgaggac tgcgcggcct
     1021 ggggcaagat gtgtcaggcc cacggcgaga acgccgaagt ccgcatgatg tcggtcaagg
     1081 gcatgacctg caccggctgc gagaactcca tcaaggccgc cctcgagaag gcgccgggtg
     1141 tcctcgaagt cgtgaaggtt gaccacaagc gcggcctcgc cgtcgtcatc gtcgacaaga
     1201 ccatggcgaa gaacgacgac atgaccaccc tcgtgaccaa caagggttac gaagctcaga
     1261 tcatcccggc cgtcgccacg accggcaccg ccgctccggc ggcgaaggtt tccgacggcg
     1321 cgaagaagtc gtgcgccgcg acctgcgcca agccgtgcga caaggccaag tcgaccgaga
     1381 ccaaggcggg cgacaagtcg acctcgaccc cgcactgatc acggcctcag gcttatagac
     1441 tgaacccgaa cggcgggagc gatcgctccc gccgttttca cgtggcctct gctcccgcgc
     1501 gcgccggttt ccgcccctgc tcctcggcat tctccttgac caaatactcc atttttggca
     1561 tcttattccc gttgacgtag ggagttcaaa tcgccggccg cccggccgca tagacgccgg
     1621 agtgtttcca tgaagtacac atttgtttct ctcgccctct gcctgatttt cgcaggttcg
     1681 atccttgccg aagatccctt gccgactcaa ccgcgaggca aaagcatacg tgagtacctc
     1741 acccccgacg gccggttcga cctcgacgcc gctcggcgct ccggctacga gggcccgctt
     1801 gatatcgaag gcttccgctc cggcatcgac cccgccaccc gcgcgccgct gtttcagcct
     1861 gcctctcccg ctttccctgc cgaccacccc gatgacatct tctgggacaa cagcatttcc
     1921 ccctccattc ccggtgttga cagcgccgta cgtgcagcta ttgtctacga tggaaatctc
     1981 gttgttggag gttcgtttca ggccgctggt gatgtggtgg cctcctacat cgcctcctgg
     2041 aatggttcgg cgtggtcctc cctcggttcg gggatgaata actccgtcta tgccctgacc
     2101 gtacacaacg gcgacctgat cgcgggggga accttcacca cggcgggcgg ggctagtgcc
     2161 aatcgcatcg cctcctggga cggttcagcg tggtcctccc tcggttcagg catgaatgac
     2221 tacgtcttgg ccctgaccgt gtacgacggc ggcctgatcg cggggggaga tttcacgatg
     2281 gcaggcggaa ccagcgccaa tcgcatcgcc tcctgggacg gttcagcgtg gtcctccctc
     2341 ggttcgggca tgagcgactg ggttcacgcc ctgaccgtat acgacggcaa cctgatcgcg
     2401 gggggagagt tcacgatggc ggggggaacc agcgccaagg gcatcgcctc ctggaacggt
     2461 tcggcgtggt cctccctcgg ttcggggatg aatggcttcg tccgtgccct gaccgtgtac
     2521 gacggcagcc tgatcgcggc gggctatttc acgacggcgg gcgggaccag cgccggcagc
     2581 atcgcctcct ggaacggttc ggtgtggtcc cccctcggtt cgggtataaa ttacgaggtc
     2641 cgtgccctga ccgtctacga cggcaacctg atcgccgggg gatatttcgt agtggcgggg
     2701 ggaaccagcg cctactgcat cgcctcctgg aacggttcgt cgtggtcccc cctcggttcg
     2761 gggatgaatg actacatcgg taccctgacc gtctacgacg gcgacctgat cgccggggga
     2821 tatttcgcaa cggcgggcgg aaccaacgcc aacttcattg cctcctggaa cggttcgtcg
     2881 tggtcctccc tcggttcggg gatggattac ggggtctatg ccctgaccgt gtacggcggc
     2941 aacctcatgg cgggaggatg gtttagcacg gcggccggaa cgagtgccaa ctacatcgcc
     3001 tcctggaacg gttcggcgtg gtcttccctc gattcgggca tgaatgacta cgtcgaggcc
     3061 ctgaccgtgt acgacggcaa cctgatcgcc gggggccatt tcgacaccgc gggcggtacc
     3121 agcgccaact tcattgcctc ctgggacggc tcggcgtggt cctccctcgg ttcggggatg
     3181 ggttactacg gctacgtcaa tggcctgacc gtgtacgacg gcaacctgat cgcgggggga
     3241 tatttcacca cggcgggcgg gaccagcgcc ggtagcatcg cttcttggga cggctcggcg
     3301 tggtcctccc tcggttcggg catgagtgac tacgacagct acgtctacgc cctgaccgta
     3361 tacgacggca acctgatcgc ggggggatat ttcaccacgg cgggcgggac caccgccaac
     3421 cacatcgcct cctgggacgg ttcggcgtgg tcttcgctcg gttcgggcat gaatgacgca
     3481 gtcgaggcct tgaccgtgta tgacggcaac ctgatcgcgg ggggagaatt cacaacggcg
     3541 ggcgggacca gcgccagtta catcgcctct tgggacggtt cggcgtggtc ttcgctcggt
     3601 tcgggcatga atgacgcggt cgaggccctg accgggtacg gcggcaacct gatcgcgggg
     3661 ggcgggttca cgacggcggg cgggaccagc gccaaccgca tcgcctcctg gaacggttcg
     3721 gcgtggtctt cgctcggttc ggggatgaat ggctacgtct tggccctgac cgtgtgcggc
     3781 ggggccctta tcgctggggg agatttcgct atagcaggca acaaggtgtc ggggcatttg
     3841 gcttcctgga cgaaatgctg caatggaacc agagggaatg tcaacttcga tgcggcggga
     3901 gcggtaaccg tggccgacat cactttcctg atcaatcatg ttttccgcgg cgggccggag
     3961 cctgactgct ttgaggaggc ggatgtaaca gcagacggga agttggcggt cgcggacatc
     4021 acgttcctca tagcgtatgt attccgcggc ggacccgcgc cggcggcgtg tccgtgagga
     4081 tgaatagggg aggcggtttc ctcgctccgt ccctgagctg ccccacgtgc gacgtcccga
     4141 tccgggtcga ggcgggggat ctcgatgagg acaacgattt cgacctggcc gcccttgcga
     4201 ctcggccgcc tgcacctcgc ttttctttaa cggcagcgac gctactttct ccgccgcata
     4261 aacgtggtct gttggcgact gcccgctcgg tatctgcgtc accgatctga ccggcgacag
     4321 cactcctgac ctcgctgtca cgggccgcga caccgacaag ctctccgtcc tcgttaaccg
     4381 gatctgcacc gacaccgagt ttccgcggcg ccagcggcgg cggggagtcg caaatggacg
     4441 agaacgatcg gcggcggcgg ttggggaagt ggggacctgc gaaaaaggcg tttcgcgctg
     4501 tcgcattcga acggcgccgc ggccccgaat ccgcgcaatc atcacaagca gggcggcggc
     4561 ggtccggtcc cgaacaggta gccgacgagg gaagtcagat cgacgacgtt gaccgggagg
     4621 agaccgtcga tgttggcggt ggccgccgac ggcggttccg gaccgcctct gaacaggtag
     4681 tcgacaaggt agatgaggtc cgcgatattc gggccgtcgc cgttatagtt gacgtctccg
     4741 gtgccgcaga tttccagctc tccggggaga aggatcggaa ccacgggacc ctgcgggcag
     4801 gagagaagcg gctcataacc gccgtatgac aggatggcaa tggggttggt ctgcccgtgg
     4861 taacctgaag agcacgtaaa gtagatgctg agcagagccc cggtgtcggg aagaatctcc
     4921 tgtcccggcg gcggattgac ctcgtaggtg gcgcacctgt tcgagggatc gcagtggagg
     4981 aaaccgtggt tcgcgatatc ggccgttcgc aggccggctg tcgacacgga gtcgaggctc
     5041 atgctcaggg ggccgttcca gtcgaacggt atcctgatgc gatccacggg ctgcgtgttt
     5101 ctcgcaccga tgtcgaatct gaccgattcc tcaacccgtc ccagtccgcc ggggacaatc
     5161 agcgtgtccg cctgcgccca tatgtagtcg ctccgggttc gggtgaaagc gccgtgggcg
     5221 gtcacaattg tgactgagac atcgtgccgc cccggtcccg tatacgtgtg gctgggatgc
     5281 ggcacatcgg ccgagtcgcc gtcgccgaaa ctccaggacc agctcaggac atcctcaagc
     5341 gtttccgaag tgaactgtac atcgagcgga acaggtccgg aggcgacatc ggcctcgaaa
     5401 ctgaccgaga cgatccagga ctccaaacga tcccgcaacc aacagtgcat gcggcctgtc
     5461 tgctgccgcg agaactcgta ccgacaccac aggggagcgt agctcatgtg attctgcggc
     5521 tgagtcggtc cccaggcgat gtccgtgcac gggtcggtcc cggagggcgg gaggcagtag
     5581 tagttttcgg gagtggggtc ggtgtcggag cagaaatccc cgacccagtc gttggtcagt
     5641 tgttgaggag actcgtagca gactccgcac ggctccacct cttcgacccc gtagaaggtg
     5701 tggtagagcc ccagggcgtg gcccatctcg tgggtgaggg tgccgtcgtc atcgggaggc
     5761 atgtgattgc tggccatgaa gataccgcct cgcggcccct cggcgccggg ctcccagggg
     5821 tacgttcccc aagagaagct ctggccgtcg atgtcgactc cggtcacgaa gatgttgcac
     5881 tggtactccg gatccaacgc gtaggtctcc ttcatgaggt tgcactcgtc ccaggagtcg
     5941 agcacccgaa actgcgatga tgaaataaac cggtaatcgt agcggaactg gatgcggtag
     6001 ggcaggaagt ctgcgttcat tgcagcgacg gcgacggcaa gattgtcggc ggaaagcgac
     6061 gggttggccc catcgtcccc ggttatgata tggaagaaca cggcaatggt agtgatcggc
     6121 tgcccgggtg cggggatcca cgaattgcgc actttcgggc tgtcacacac accctcaggc
     6181 gggcacatga accccgccgt tttccgccca tactgccaat cataccagaa ttgcgtgagg
     6241 caccggtgat cgtcgcgttt cggaccagag ggattcccgg gcctggccga ctgcgcgtcg
     6301 gtactcactg tggtcagaca gaacaggata accgcacaga acgcactacg catcaacgac
     6361 ttccgttctc gctcgtttcc ggatctgcga tccactgtgg cctttccttg cctgatttat
     6421 tcacaggccg gccgcctgaa cattgagtca gagaaggctt ccacaggtta tttatccaaa
     6481 aacgtgggct tggttgctgt atatcaaagc cgaaaacaaa tccgcaggaa atgcgaaatt
     6541 gggcagcggc aggcaaggcc tcagacgagt cggattcgat ctgttcagcg tcccctgctg
     6601 cctttgccca taggccggaa accctcagcc tccggcaaat gacggcatag ctcaggcgtc
     6661 tccgccgaca agctctaact tcatagcccg caatgcaggc ctttcgggcg ccaccggcgc
     6721 agcactcggc cccgccgata cttacagtag cgtcccgact ctccgtatta tatcttgccg
     6781 ccgatggtg
//
LOCUS       DAOPOE010000134         6709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12544_length_6709_cov_5.204539, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000134 DAOPOE010000000
VERSION     DAOPOE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_6709_cov_5.204539"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKM94_12875"
     CDS             <1..266
                     /locus_tag="PKM94_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOD67569.1"
                     /translation="SDEEKVMILKSLPDQGVSVQRYKGLGEMNPEQLWRTTMNPETRT
                     LLQVTIDDAAEADHLFSVLMGTEIAPRSTFIQENARYVRNLDI"
     gene            complement(593..1072)
                     /locus_tag="PKM94_12880"
     CDS             complement(593..1072)
                     /locus_tag="PKM94_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67570.1"
                     /translation="MSFFGGSTEFPKGEAALAEDEAAVLDKLALKVVDWQMTAPAIIF
                     LESIKPLNFIGSQAMVFFEPIIQSVFAFKDYDTLRTALEKRSAIEVLLQRIEAHDAVA
                     KAREQRIKKYRREQKKTWKWYQRYLGVLQPRLTYPDDVLYGEEGKKPGDAGREAEKP"
     gene            complement(1069..1494)
                     /locus_tag="PKM94_12885"
     CDS             complement(1069..1494)
                     /locus_tag="PKM94_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67571.1"
                     /translation="MEWRVHPATRRPWLTAAVSLFILAVSFLVLVTTDSRWFSFFALV
                     VLFASLAKFYFPTSYRMDGRGVTIKTTTQTLLKEWRLFRSYYPDRNGVLLSPFPQPSR
                     LENFRGLYVLFGGNREDVVAFVSRHVGAAAPAGDKEATP"
     gene            complement(1568..1774)
                     /locus_tag="PKM94_12890"
     CDS             complement(1568..1774)
                     /locus_tag="PKM94_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67572.1"
                     /translation="MEERKYALKMVVNGKEREVSFEELALSNNLAQEALVRLLIEKKV
                     FQPEEMLRMLEKVKDERYRSPDQR"
     gene            complement(1828..3597)
                     /locus_tag="PKM94_12895"
     CDS             complement(1828..3597)
                     /locus_tag="PKM94_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOD67573.1"
                     /translation="MPARIAWYVLLAAALWSATGRAAEPPVDPPAAAGDWEQGQRLLR
                     SGDYAGAARLFEQLAAQHPASPALPLFVFSRAKANYYGGEYAKAAAGFSQLLSRFPRA
                     PEVPYAHFFLGNTACRRGDLDRALHDYLEAYRLSTDDRLDKLAQESVRALLSGDGAVT
                     VTAADFLNIPETRRCRLIDDLEEPLRRRGEAGVAAELMASCGWTLDTAGIDRSDAGAD
                     AIEVAVVLPLSGELQEFGNEIYRGALVGASMYAAQSGRQVVLAPYDTEGDAIAAARLV
                     GELAGGTADGVIGPLTSEEAAVAAARLHGADLPLVIPAATDAGLTRLSESSFQLSPNI
                     ELQAVRMADYAIDVLRAATAAIIAPAGGEHQRMAERFAERFRQRGGAVAASEYYGPRD
                     RDYGPCLRDLKGALLGEVADSSVWLDNRGDTIEADGIAAEVDVLYLPGRAEQIRMLLP
                     QLRFHNLRGRYLGSDGWGDEEIFRLGDDVTQHAVFPSPFLPAPPSEEAMRFAAAYDER
                     YGAEPERLARLGYDAMQLIVRAVERGRDSRRETARLLAAVRDYAGAAGAVTFGEQREN
                     VAIPLYRIADGAAVPLAEPAPAE"
     gene            complement(3597..4112)
                     /locus_tag="PKM94_12900"
     CDS             complement(3597..4112)
                     /locus_tag="PKM94_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOD67574.1"
                     /translation="MTFLEVKVDGLALDMTTNSPVVILSPAGLNKVLPIWIGHAEAWG
                     IAMELSGVKPKRPMTYDLMKVMLATVAVTIDRVEVTELREQTFYARIHLRRNGQVFEV
                     DARPSDSIAMALKTGAKIFVNEQLFNLEATAESGGGEESPEAPPMSTDPESLRERLRR
                     INPEDFGNYSL"
     gene            complement(4109..4558)
                     /gene="rplI"
                     /locus_tag="PKM94_12905"
     CDS             complement(4109..4558)
                     /gene="rplI"
                     /locus_tag="PKM94_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOD67575.1"
                     /translation="MKVILRQDVPDVGAAGQTVEVKTGFGRNYLIPRNLAIPATKASL
                     RAIDEIRKQTEIREKKARREAEVVKDRIEKVSLTHEVLVGEDDRLYGSVTNADIAERL
                     AAEGITVDKRAIQLDTPIKVLGIYTVPVKIAKDVTANLKLVVVKKSE"
     gene            complement(4598..5599)
                     /locus_tag="PKM94_12910"
     CDS             complement(4598..5599)
                     /locus_tag="PKM94_12910"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOD67576.1"
                     /translation="MDAAHTRGTTATVAAALLFYAALLYAGMADHGRPLAVLAAAPVM
                     YFTAMYLYYGLTRLAFARRQWALWGSAVAAVAVSYLLLGGSHLWMLVFGWGMLLVTAV
                     LTGRLTSGGYRPRTVYIAAVLSLVAFGSALYLPLWLDLVRAMPKSIETVVDAARERLA
                     AAGESDVQVEQLTEAFRRLATVVLRLAPAAMLLSAVVQFSVGYLLFVKWVDRCQAGRP
                     QYEPFHFWRMPYGFVPVTAAAAAVRLLGGAAVQLAADNALAFLAVFYCLTGLSFVEFY
                     LRRVQFTRFMKVLFYVFLALLPLVYPVVGVIAFGAIALVGFADSFADWRRLRLREFE"
     gene            complement(5631..5849)
                     /gene="rpsR"
                     /locus_tag="PKM94_12915"
     CDS             complement(5631..5849)
                     /gene="rpsR"
                     /locus_tag="PKM94_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOD67577.1"
                     /translation="MRRKKFCFFCENKLAGADYRDERSLRRFVNERGKIIPRRISGNC
                     ALHQRRLTIAIKRARMLAILAFESESFR"
     gene            complement(5884..6372)
                     /gene="rpsF"
                     /locus_tag="PKM94_12920"
     CDS             complement(5884..6372)
                     /gene="rpsF"
                     /locus_tag="PKM94_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOD67578.1"
                     /translation="MRVYETTFIVNPQTDDATIDQQVVAISDLITRHGGRILEEERVG
                     TRRLAYPIRKLTQGYYHSFVYEAPQQLLPDLDRYFRLNESYLRFMTIAFEGDVEAVRR
                     RAAERPADMREPAKTTAEPAAAAEPAAAAEPAAIENGEGAGTDDLDDDFDEDADEEYR
                     GR"
     gene            complement(6443..>6709)
                     /locus_tag="PKM94_12925"
     CDS             complement(6443..>6709)
                     /locus_tag="PKM94_12925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD67579.1"
                     /translation="RTGGSDGGHNGLASIIATLGTQRFPRLRLGIGPAPEGVDPADFV
                     LAKFEKSERTPAAEMIDTAAEAVLYSIDHRFEEAMNIFNANPAR"
BASE COUNT         1078 a         2454 c         2075 g         1102 t
ORIGIN      
        1 acagcgacga agagaaggtg atgatcctca agtcgctccc ggatcagggt gtctcggtgc
       61 agcgctacaa gggtctcggc gagatgaacc ccgaacagct ctggcggacg acgatgaacc
      121 cggagacgcg cacgctcctg caggtgacga ttgacgacgc cgccgaggcc gatcacctct
      181 tcagcgtgct catgggaacg gagatcgcgc cgcgcagcac gttcattcag gagaacgcgc
      241 ggtacgtgcg caatctcgac atctgatccc ggccgcgcgc gagcaccggt tcgcgggccg
      301 gcctgcgccg gcccggccgt gggcccgccc gctctccgcc ccggaaagaa tcctcccttc
      361 cccggcgcgg cgccgtatat taatcctgta acccaactcg gaggtgtacg gtgaaaatca
      421 gcggttggtg cgtgctcatc ctcgtcctcc tcgccgtgat tgtgtggctc gccacgctcg
      481 acgaggagga cttcgcccgg tcctgggcca aggtcaagaa ggactgacgg gttgttctga
      541 cgccggcggc gccgggacgt ggccgcctga actcgcgccg gatgacggcc ccctacggtt
      601 tctccgcttc ccgtcccgcg tcccccggtt tcttcccctc ctccccgtag agcacgtcat
      661 cggggtacgt aaggcgcggc tgcaaaactc ccaggtagcg ctggtaccac ttccaggtct
      721 tcttctgctc ccggcggtac ttcttgatcc gctgctcgcg cgctttggcc accgcgtcgt
      781 gcgcctcgat ccgctggagg agcacttcga tcgccgaccg cttctcgagc gccgtgcgga
      841 gggtatcgta atctttgaag gcgaacacgc tctggatgat cggttcgaaa aacaccatgg
      901 cctgcgaccc gatgaaattg agcggtttga tcgactccag gaaaatgatc gccggcgccg
      961 tcatctgcca gtccaccacc ttcagagcga gcttgtccag caccgccgct tcgtcctcgg
     1021 ccagcgccgc ttcccctttc ggaaactccg ttgatccgcc gaagaaactc acggcgtcgc
     1081 ctccttgtct ccggccggcg ccgccgcccc tacgtgccgg ctcacgaagg ccaccacgtc
     1141 ctcccggttc cccccgaaca gcacgtacag cccgcggaaa ttctccagcc gcgacggctg
     1201 cgggaagggg gagaggagca cgccgttgcg gtcgggatag tagctccgga aaagccgcca
     1261 ctccttgagc agcgtctgcg tcgtcgtctt gatcgtcacc ccgcgcccgt ccatgcggta
     1321 gctcgtgggg aaatagaact tggccagcga ggcgaacagc accacgaggg cgaagaacga
     1381 gaaccagcgc gaatcggtgg tcacgaggac aaggaagctc acggcgagga tgaacaggct
     1441 caccgccgcc gtcagccacg gccgcctcgt cgccgggtgt acccgccact ccatcgccgg
     1501 gccgtccgcc gggaccgctt gcgtcccgcc ggcgcgttcg gccggggtgt ttgctccgcc
     1561 gtccatacta ccgctgatcg ggcgaacggt accgctcgtc cttcaccttc tccagcatcc
     1621 gcagcatctc ctccggctga aacactttct tttcgatgag cagccggacg agcgcctcct
     1681 gggcgaggtt gttcgacagc gccagctcct cgaacgacac ctcgcgttcc ttgccgttga
     1741 ccaccatctt gagcgcgtac ttccgttctt ccacttccga ctccagtccg tccgttacag
     1801 gtttcgtttg cggcgcaggg gcgggggtca ctccgcgggc gccggttcag caagcggcac
     1861 cgccgcgccg tcggcgatgc ggtaaagcgg gatcgccacg ttctcgcgct gttcgccgaa
     1921 cgtcaccgcc cccgccgcgc ccgcgtagtc gcgtaccgcg gccagcaggc gcgccgtctc
     1981 gcggcgcgag tcgcgccccc gctcgaccgc ccgcacaatc agctgcatcg cgtcgtagcc
     2041 cagccgggcc aggcgctccg gttccgctcc gtaccgctcg tcgtaggccg ccgcaaaccg
     2101 catcgcttcc tcggacggcg gcgccggcaa gaacggcgag ggaaacaccg cgtgctgtgt
     2161 tacgtcgtcg ccgagccgga agatctcttc atccccccat ccgtccgacc cgagatacct
     2221 cccccgcagg ttgtggaacc ggagctgcgg gagcagcatg cggatctgtt ccgcccgccc
     2281 cgggaggtag agcacgtcca cctccgccgc aatcccgtcg gcttcgattg tgtcgccgcg
     2341 gttgtccagc cagaccgatg aatccgcgac ctcgccgagg agcgccccct tgaggtcgcg
     2401 gaggcagggc ccgtagtccc tgtcgcgcgg gccgtagtac tcgctcgccg ccaccgcgcc
     2461 gccccgctgc cggaagcgct cggcgaaccg ctccgccatc cgctggtgtt cgccgccggc
     2521 cggggcgatg atcgccgccg tcgccgcccg cagcacgtcg atcgcgtagt cggccatccg
     2581 cacggcctga agctcgatgt tgggggagag ctggaacgag gattccgaga ggcgggtgag
     2641 ccctgcgtcg gtggccgccg ggatgaccag cggcagatcg gcgccgtgca gccgggccgc
     2701 cgccaccgcc gcctcctcgc tcgtgagcgg tccgatcacg ccgtccgccg tgccgcccgc
     2761 cagctcgccg accagccggg ccgcggcgat cgcgtccccc tctgtatcat agggcgccag
     2821 cactacttgc cggccgctct gggcggcgta catcgaggcc ccgacgagcg ccccgcggta
     2881 gatctcgttg ccgaattcct gcagctcgcc cgagagcggc agcacgaccg ccacttcgat
     2941 ggcgtcggcg ccggcgtccg accggtcgat cccggcggtg tcgagggtcc acccgcacga
     3001 ggccatgagt tcggccgcga cgcccgcctc gccgcgccgc cgcagcggct cctcgagatc
     3061 gtcgatcagc cggcagcgcc gcgtctccgg gatgttgagg aaatccgccg cagtgaccgt
     3121 caccgcgcca tcgccgctca gcagcgcccg gaccgactcc tgcgccagct tgtccagccg
     3181 gtcgtcggtc gacagacggt aggcctcgag gtagtcgtgc agcgcgcggt caagatcgcc
     3241 gcgccggcag gcggtgttgc cgaggaagaa atgcgcgtag ggaacctccg gcgcccgggg
     3301 aaagcgcgac agcagttgtg agaacccggc cgccgccttg gcatactcgc cgccgtagta
     3361 gttggctttg gcccgcgaaa agacaaacag cggcagcgcg ggggaggcgg ggtgctgcgc
     3421 ggccagttgc tcgaagagcc gggccgcccc ggcgtagtcg ccactccgca gcaggcgctg
     3481 cccctgctcc caatcgccgg ccgccgcggg cgggtcgacc ggcggctcgg ccgcccggcc
     3541 ggtcgccgac cacagggccg cggccagcag cacgtaccac gctatccgcg caggcatcag
     3601 agcgagtagt tcccgaaatc ttccgggttg attcttctca gccgctcgcg cagcgactcc
     3661 gggtcggtcg acatcggcgg cgcctccggc gactcctcgc cgccgcccga ctccgccgtc
     3721 gcctcgagat tgaacagctg ctcgttgacg aaaatcttcg cacccgtctt cagcgccatc
     3781 gcaatcgaat ccgagggccg ggcgtccacc tcgaacacct gaccgttgcg ccgcaggtgg
     3841 atgcgcgcgt agaacgtctg ctcccgcagc tccgtcacct cgacccggtc gatcgtcacc
     3901 gccaccgtcg cgagcatcac cttcatcagg tcgtacgtca tcggccgctt cggtttcacg
     3961 cccgacagct ccatcgcgat gccccacgcc tcggcgtggc cgatccagat cggcagcacc
     4021 ttgttgaggc cggccgggga caggatgacc accggggaat tggtggtcat gtccagcgcc
     4081 agaccgtcca ctttcacttc gagaaacgtc attcgctttt cttcaccacc accagcttca
     4141 aattcgccgt cacatccttg gcgatcttga ccggcaccgt gtaaatgccg agcaccttga
     4201 tcggcgtgtc cagctgaatg gcgcgcttgt ccaccgtgat cccctcggcc gccagccgtt
     4261 cggcgatgtc ggcgttggtg acgctgccgt aaagccggtc gtcttcgccg accagcacct
     4321 cgtgcgtcaa ggatactttc tcgatccggt ctttgaccac ttcggcctcg cgccgggctt
     4381 tcttctcccg gatctccgtc tgcttgcgga tctcatcgat cgcccgaaga ctggccttgg
     4441 tcgccggaat cgcgaggttg cgggggatga ggtagttccg gccgaacccg gtcttcacct
     4501 ccaccgtctg ccccgccgct cccacatccg gcacgtcctg acgcagtatc actttcatgg
     4561 tacacccttt ctcacaccta tgcaacggat attgatctca ttcaaattcg cgcagccgga
     4621 ggcgccgcca gtcggcgaag ctgtcggcaa agccgaccag ggcgatcgcg ccgaacgcga
     4681 tcaccccgac caccggatac accagcggca gcagcgcgag gaacacatag aacagcactt
     4741 tcataaagcg tgtaaactgc acgcgccgca ggtagaactc cacgaacgac aacccggtca
     4801 ggcagtagaa gaccgccagg aacgccagcg cgttgtcggc cgccagctgc accgcggcgc
     4861 cgcccagaag gcgcaccgcc gccgccgccg ccgtcaccgg cacaaacccg tacggcatcc
     4921 gccagaaatg gaacggctcg tactgcggcc gccccgcctg gcagcggtcc acccacttga
     4981 caaagagcag gtagccgacg ctgaactgca ccacggccga caggagcatg gccgccggcg
     5041 ccagccggag caccaccgtc gccagccgcc ggaacgcctc cgtcaactgc tccacctgca
     5101 cgtcgctctc gcccgccgcc gccagccgct cgcgggccgc gtcgaccacc gtctcgatgc
     5161 tcttgggcat cgcccggaca aggtcgagcc agaggggcag gtagagggcg ctgccgaacg
     5221 cgacgagcga caggacggcc gcgatataga ccgtccgcgg gcggtagccg cccgaggtca
     5281 gccgtcccgt gagcaccgcc gtcaccagca gcatgcccca gccgaacacc agcatccaga
     5341 ggtgcgatcc gcccagcagc aggtagctga ccgcgaccgc ggcgaccgcc gacccccaca
     5401 gcgcccactg ccggcgggcg aacgccagcc gggtcaggcc gtagtagagg tacatggcgg
     5461 tgaaatacat gaccggcgcc gccgccagca ccgccagcgg gcgcccgtgg tccgccatcc
     5521 cggcgtacag cagcgccgcg taaaacagca gagccgccgc caccgtcgcc gtcgtcccgc
     5581 gcgtgtgcgc cgcgtccatg gtcgccgtcc tcgtgcccga gccgtcccgc ctagcggaac
     5641 gattcgctct cgaacgccag aatcgccagc atccgcgccc gcttgatcgc aatcgtcagg
     5701 cggcgctggt gcagcgcgca gttgccggaa atgcggcgcg ggatgatctt gccgcgctcg
     5761 tttacgaagc ggcgcagcga ccgctcatcc cggtagtccg ccccggccag cttgttctcg
     5821 cagaaaaagc agaatttctt ccgtctcatc gtacgcataa ttacaccttc tcactgccgc
     5881 ggcctaccgc ccgcggtact cctcatccgc atcttcatcg aagtcgtcgt ccaggtcgtc
     5941 ggtaccggcg ccttcgccat tctcgatcgc cgccggttcg gcggccgcgg ccggttccgc
     6001 ggcggccgcc ggttcggccg tcgtcttggc cggctcgcgc atatcggccg ggcgttccgc
     6061 cgcccgccgc cgcactgcct ccacgtcgcc ctcgaacgcg atcgtcatga accgcaggta
     6121 cgactcgttg agacggaaat agcggtcgag atccggaagc aactgctggg gcgcctcgta
     6181 gacgaacgag tggtagtacc cctgggtcaa cttgcggatc gggtaggcca gccgccgtgt
     6241 gcccacccgc tcttcttcca ggatgcgccc gccgtggcgg gtgatgagat cggaaatcgc
     6301 caccacctgt tggtcgatcg tggcatcatc ggtctgggga ttgacaataa atgtcgtttc
     6361 gtaaacgcgc attcactgaa ctcccttcgg tcgttagttt aaggttcaac aaacggctcg
     6421 gtatggtccg ccggggggcg gcctaccggg cagggttcgc gttaaatatg ttcatcgctt
     6481 cttcgaagcg atggtctatg ctgtacagaa ccgcctcggc ggcggtgtcg atcatctcgg
     6541 ccgccggcgt gcgctcgctt ttctcgaact tcgcgagcac gaaatccgcc gggtcgaccc
     6601 cctcgggcgc cggtccgatc cccaaccgca gccgggggaa ccgctgggtc ccgagggtcg
     6661 cgatgatcga ggccaggccg ttgtggccgc cgtcactgcc ccccgtccg
//
LOCUS       DAOPOE010000135         6700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12572_length_6700_cov_6.555004, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000135 DAOPOE010000000
VERSION     DAOPOE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12572_length_6700_cov_6.555004"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKM94_12930"
     CDS             <1..1023
                     /locus_tag="PKM94_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67580.1"
                     /translation="WMTGRMAFGLVLLALGLLLCAGCGSDDDDGGANGPAPLPRIEAF
                     TLVSDSPVRRLIDSLGTDSWTGVDSVIATVAPTNAPKLTPGAGASLPTTAKVQAVRTS
                     THLFLRLQWVDNSFSSYPAYWTVTTTGPGVIFSGPALDEEDQAVVLFQSPTAGQYDVW
                     NWKVLTTGAAHMGEGYSLAGTVWTEDVVGSAPSVEPAMLNRQTQFTAPEWLSKDTCEF
                     EGPVLYLADASNDPDLRLTTNWTIGDTLPGWVIDSSIAADFRTDEQRGSRWDIFAGGA
                     YDPDGDMYSVVLGRKLNTTFEDDLDLSTLDSVQVMLGFYDNQNDLALGGSHRSFSQRF
                     WLVLPH"
     gene            1172..2638
                     /locus_tag="PKM94_12935"
     CDS             1172..2638
                     /locus_tag="PKM94_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOD67581.1"
                     /translation="MALKTYEQYLASLRRMRPNIYKFDELISDVTAHPATKRTVEGHG
                     WTFKAAADPELAPLVTATSHLTGEPISRYLSIIQTPEDMWANSAMKRLMFQLTGTCTG
                     GRCAGWCALNTMFITTWEMDRDLGTNYHARLLAWLKDAQARDITISGALTDAKGDRTL
                     KPHEQDDPDVFLRVVEKRPDGIVVRGAKVMICGVAAANEIFILPGSAYKEEDADYCLS
                     FVIPRDIAGLTIVETRHPSDGRDLEEGWDNPITEGGITQAFLLFEDVFVPSERVFMLG
                     EHPYTGKTVANFIMPYRSAIGGCVAGQGDIKIGSAILTARTNGLSAKVFSDKLVQMHI
                     NNETTYGLGIAAAALGKKHPSGAWLCDPLLSNVNKVHVATLPYETSVLAQDIAGGIAE
                     TGCMPSWKDFQSKKYGHLIKKYMKAKHSAEARARAARLVEWCTIGGGVPGCMHGGGSP
                     DGAKLVIRASADLEKKVEIARRLAGIAEEVPDPGAKKK"
     gene            2802..4325
                     /locus_tag="PKM94_12940"
     CDS             2802..4325
                     /locus_tag="PKM94_12940"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOD67582.1"
                     /translation="MTAREAYTRLKARMKEIAVIESTGGVLYWDRKSYMPPKGIAHRS
                     EQLAQLTRLSHDWFTAPEVGDWLAAVEQSDLVRDPASEAAVNVREWRRLRDREEKLPA
                     AFVEQFARTTALAGQVWAEARKKSNFALFEPHLGGIVELVRRKADYYGYTAEKYDALL
                     EGYEPGARTAEVERAFAGLRTDLVELVEKIADAPRRPDIGVLKRPWDVDKQRTFSRML
                     AAQIGYDFGAGRIDETVHPCCNDLGPLDVRILTRFYAEDLAEGLTGTIHEAGHAFYTQ
                     SQRGSEHWGTPMGMTPSLAIHESQSRTWENIVGRSRHFWTYFFPQLRALFPEETRDIT
                     LDDFYAAMNYVAPSYIRVEADEATYNLHIMLRFELERAIVEGDLAVKDIPGEWNSRFR
                     QYLGLAVDTDAHGCLQDVHWSDGSFGYFPTYALGNLYAAQFWSRAKADLPGLMEDFAA
                     GQFDRLMTWLSENIHRQGFKVYPNELCRRLTGSDLSHRPLLDYLYAKYEEIYGIRRN"
     gene            complement(4399..5250)
                     /locus_tag="PKM94_12945"
     CDS             complement(4399..5250)
                     /locus_tag="PKM94_12945"
                     /inference="COORDINATES: protein motif:HMM:NF020302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
                     /protein_id="HOD67583.1"
                     /translation="MDRKREDTIVFTEDHRRYGAFCLGAPYVIRIDDVSWPTDRHYLL
                     GMKYPGVNPLRLRQTASYAEAVKLVQTEGAVERVKWDVVRDDYLERVLRAKFSQHLDL
                     EALLRSTGDHPLVYGNADDSYYGVGASGAGHNMFGRILMRVRGRVDRSVPEPHNAVIE
                     GCEEWVRRNPDDMSGFLRLTIVYLANRMPERASATAYRAIHLDPQDPRARIALAYALV
                     DQHRFGEALEHLRFVNRRGEADEDDLRFLATVYRALGRDVAADVLESRARRLEAEEED
                     DCDGDDF"
     gene            complement(5887..>6700)
                     /gene="thyX"
                     /locus_tag="PKM94_12950"
     CDS             complement(5887..>6700)
                     /gene="thyX"
                     /locus_tag="PKM94_12950"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOD67584.1"
                     /translation="FRVLDHGFVRLIDYMGGDESIVQAARVSYGKGTKKVSEDRGLIR
                     YLLRHRHTTPFEMVEFKFHVKLPIFVARQWIRHRTANVNEYSGRYSVMKEEFYIPPEE
                     EIRFQSAVNKQGRAEEEVPPELKRRFVEFLRKIQADSYAEYAALVEAGLARELARVGL
                     PLSLYTEWYWKIDLHNLFHFLGLRLDRHAQQEIRAYAAVMAEMVQAVCPLAYEAFLDY
                     QLQAVHFTGPELAILRPRLTDFCPSEDELASGGLSKREAREFLEKLEKVRGE"
BASE COUNT         1238 a         2326 c         1998 g         1138 t
ORIGIN      
        1 tggatgaccg gccggatggc cttcggtctc gtcctgctcg cgctcggact gctgctgtgc
       61 gccgggtgcg gcagcgatga tgacgacggg ggggcgaacg ggccggcccc gctcccgcgc
      121 atcgaggcat tcaccctggt cagcgattcg ccggtccgcc ggctgatcga ttcgctcggc
      181 accgattcct ggaccggcgt cgacagcgtc atcgccacgg tcgcgcccac caacgccccc
      241 aagctcactc cgggcgcggg cgcctcgctg ccgacaaccg ccaaagtcca ggcggtgcgg
      301 acatccaccc acctgttcct gcgcctgcag tgggtcgaca actcgtttag ttcctacccc
      361 gcctactgga ccgtcaccac caccggcccc ggcgtcatct tcagcgggcc cgccctcgac
      421 gaggaagatc aggcggtcgt gctgttccag tcgcccaccg ccgggcagta tgacgtgtgg
      481 aactggaagg tgctcacgac cggcgcggct cacatgggcg aaggctactc gctcgccggc
      541 acggtctgga ccgaggacgt ggtcggcagc gcaccgagtg ttgagccggc catgctgaac
      601 cgccaaaccc aattcactgc gccggagtgg ctgagcaagg acacctgcga gtttgagggc
      661 cccgtgctgt atcttgccga cgcgtcaaat gaccccgact tgcggcttac caccaactgg
      721 acaatcggcg acacgctccc cggctgggtg attgactctt cgatcgccgc cgatttccgc
      781 accgatgagc agcggggaag ccgctgggac attttcgccg gcggcgccta tgacccggat
      841 ggcgacatgt actcggtggt gctgggacgc aaactgaata cgactttcga ggacgacctc
      901 gatctgtcca ccctcgattc cgttcaggtc atgctcggct tctacgacaa ccagaatgac
      961 ctcgccctcg gcgggagcca ccgctccttc agccagcgct tctggttggt cctcccccat
     1021 tagcgcggcc attcggagtt ttggacaagg ccggttttcg ctttcggaaa ccggccttcc
     1081 tgttgctccg cgcgctttct tcttgtgctc tttttcacaa tctcctatat tcggatcagc
     1141 attgataact ttagcctatt ctggaggagc catggccctg aagacctacg agcagtacct
     1201 cgcatccctc cgccggatgc gcccgaacat atacaagttt gacgaactga tcagcgacgt
     1261 cacggcccac ccggccacca agcggacagt cgagggccac ggctggactt tcaaagccgc
     1321 ggccgacccg gaactcgccc cgttggtcac cgcgacctcg catctgaccg gcgagcccat
     1381 cagccgctat ctctcgatca tccagacacc cgaggacatg tgggcaaatt cggccatgaa
     1441 gcgcctcatg ttccagctca ccggcacctg cacgggcggc cggtgcgccg gctggtgcgc
     1501 gctcaacact atgttcatca ccacctggga gatggaccgc gacctcggca cgaattacca
     1561 cgcgcgcctc ctcgcctggc tcaaagacgc ccaggcccgc gacatcacca tttccggcgc
     1621 cctgaccgat gcgaaggggg accgcaccct caaaccgcac gagcaggatg accccgacgt
     1681 gttcctgcgc gtcgtcgaga aacgccccga cggcatcgtc gtccgcggcg ccaaagtcat
     1741 gatctgcggc gtcgccgccg ccaacgaaat cttcatcctc cccggctccg cttacaagga
     1801 agaagatgcc gactactgcc tctcgttcgt catcccgcgc gacatcgcgg gcctcaccat
     1861 cgtggagacc cgccatccct ccgacggccg cgatctcgaa gagggctggg acaacccgat
     1921 caccgagggc ggcatcaccc aggcgttcct gctgttcgag gatgttttcg ttcccagtga
     1981 gcgcgtcttt atgctcggcg aacacccgta cacggggaag acggtcgcca acttcatcat
     2041 gccttaccgc tcggcgatcg gcggttgcgt cgccggacag ggggacatca aaatcggatc
     2101 ggcgatcctc accgcccgca ccaacggcct ctcggccaag gtgttcagcg acaaactcgt
     2161 ccagatgcac atcaacaacg agaccaccta cggtctcggc atcgccgccg ccgccctggg
     2221 aaagaaacac ccctccggcg cctggctgtg cgacccgctt ctgtccaacg tcaacaaggt
     2281 ccacgtggcg accctgccct atgaaacctc cgtgctcgcc caggatatcg ccggcggcat
     2341 cgcggaaacc ggctgcatgc cgagctggaa agacttccag tcgaagaagt acgggcacct
     2401 gatcaagaag tacatgaaag cgaaacactc ggccgaggcc cgggcccggg cggcgcgcct
     2461 ggtcgaatgg tgcaccatcg gcggcggcgt ccccggctgc atgcacggcg gcggttcccc
     2521 cgacggggcc aagctcgtca tccgcgcctc ggccgacctc gaaaagaaag tcgaaatcgc
     2581 ccgccgcctc gccggcatcg ccgaggaagt ccccgacccg ggcgctaaaa agaagtaagc
     2641 cgagagctct cggcccccct gtgatcgctc cgcaacgcgg gagcctcccg gctcccgccc
     2701 tccggtcccg tgcgctccac cggaccgtca tttccattgc ccccggccca cgccgcgcct
     2761 atcattgaac acacgcgtta ccgttgcaga aggagtctcc catgaccgcc cgcgaagcct
     2821 acacccggct caaagcccgc atgaaagaga tcgccgtcat tgaatccacc ggcggcgtgc
     2881 tctactggga tcgcaaatcg tacatgcccc cgaaagggat tgcccatcgc tcggaacagc
     2941 tcgcccaact gacgcggctc agccatgact ggttcaccgc cccggaggtc ggggactggc
     3001 tcgccgcggt ggagcagtcg gacctcgtgc gcgatccggc atcggaagcg gcggtcaatg
     3061 ttcgcgagtg gcggcgtctc cgcgatcggg aggagaaact gcccgccgcc tttgtcgagc
     3121 agttcgcccg gacgacggcg ctggccggcc aggtgtgggc ggaggcgcgg aaaaagtcga
     3181 atttcgcgct gttcgagccg cacctgggcg ggattgtcga actcgtccgc cgcaaggcgg
     3241 actactacgg gtacaccgcc gagaaatacg acgcgctgct ggagggctac gagccggggg
     3301 cgaggacggc cgaggtcgaa cgggcgttcg ccggtcttcg caccgacctc gtcgaactgg
     3361 tggagaaaat cgccgacgca ccccggcggc cggacatcgg cgtgctcaaa cgcccctggg
     3421 atgtggacaa gcagcgcact ttctcccgca tgctcgccgc tcagatcggc tacgatttcg
     3481 gagccgggcg gatcgacgaa accgtccacc cctgctgcaa cgacctcggc cccctcgacg
     3541 tgcggatcct cacccggttc tatgccgaag acctcgccga agggctcacc ggcaccatcc
     3601 acgaggccgg acacgctttc tacacgcaga gccagagagg ttcggaacac tgggggacgc
     3661 cgatgggcat gactccctcg ctggctatcc acgagtcgca gtcccgaacc tgggagaaca
     3721 tcgtcggccg ctcgcgccac ttctggacgt atttcttccc ccagctcaga gcgctgttcc
     3781 ccgaagaaac ccgggatatc acgctggacg acttctacgc cgcgatgaac tacgtcgccc
     3841 cgtcgtacat ccgcgtcgag gccgacgaag ccacctacaa cctccacatc atgctgcggt
     3901 tcgagctgga acgcgcgatc gtcgaggggg atctggcggt caaagacatc cccggcgagt
     3961 ggaacagccg cttccgccag tacctcggcc tcgctgtcga cacggacgcc cacggctgcc
     4021 tgcaggatgt ccactggtcg gacggctcgt tcggatattt cccgacctac gccctcggca
     4081 atctctacgc cgcgcagttc tggtcgcggg cgaaggccga tctgcccggc ctcatggagg
     4141 acttcgcggc cgggcaattc gaccggctca tgacctggct cagcgagaac atccaccggc
     4201 agggattcaa ggtgtacccg aacgagctgt gccgccgcct caccgggtcg gatctgtccc
     4261 accgcccgct gctggattac ctgtacgcca agtacgagga gatctacggg atccggcgga
     4321 actgagcgcc ccgggcgcaa tccaagtgcc ggcgccggga gagatcaggg ccgccggcgg
     4381 ccccgacctt tcctccgctc agaagtcgtc tccgtcgcag tcgtcctcct cttcggcttc
     4441 caggcggcga gcgcgcgact cgagaacgtc ggcggcaacg tcacgcccca gggcgcggta
     4501 gacggttgcg aggaagcgca ggtcatcctc atccgcctcg ccgcggcgat tgacgaaccg
     4561 caggtgttca agcgcttcgc cgaagcggtg ctggtccacc agggcgtagg cgagagcgat
     4621 ccgcgcccga ggatcctgcg ggtcaagatg aatcgcccgg taggcggtcg ccgacgcgcg
     4681 ctcgggcatc cggttcgcaa gataaacgat cgtgagcctg aggaaaccgc tcatatcgtc
     4741 cgggtttcga cgcacccatt cctcacagcc ctcgatgacc gcattgtggg gctccggaac
     4801 gctccgatcg acgcgcccgc ggacgcgcat aaggatccgg ccgaacatgt tgtgcccggc
     4861 tccgctggcg ccaaccccgt agtacgaatc atccgcattt ccgtagacca ggggatggtc
     4921 gcccgtgctg cgaagaagcg cctcgaggtc gaggtgctgc gagaacttcg cccggagaac
     4981 ccgttcgagg taatcgtcgc gcaccacatc ccatttcacc cgctcgacag cgccctccgt
     5041 ctgcaccagt ttcacagcct cagcgtatga cgccgtctgc cgcagacgga gagggttcac
     5101 gccggggtac ttcataccga gcaggtagtg gcggtcggtc ggccacgata catcatcgat
     5161 gcggatgacg tagggggcac caaggcaaaa cgcgccgtag cggcggtggt cctcggtgaa
     5221 cacgatcgtg tcttcgcgct tgcgatccat ctggttctca acctccatgg tccacgaaca
     5281 tcataccgtg acttgggcac acgcgaccct ctgcgggcga gaacaacccg aacacacggg
     5341 tcgacctcgg ccactagcga gccatgtcat tgtgccgacg gtaacctgtc ggggcggggc
     5401 tgagaactgc aggccttcac gggcatcccc gtgcaggcgc gaagcggatt cgagcggaaa
     5461 aaaacctcag cccgaccgtc aggccacctt acctagcgga attcgccagg ctggtctatc
     5521 agttgcgtgg agactcatgt ggcaacctct ggtcccggac catgggcccg gtgagtaaga
     5581 gtttacggtc attcccccgg cacacggccg agggcaggct ttccccgcac agaaaagtaa
     5641 tgctacgtgg tgagcatagt acgcttctct ggtggcggag tttcctattt tatacgcgcg
     5701 gccgcgcgtg tcaagcgaaa tttccagaaa agttgcggtg cgggatgggt cgccgggagc
     5761 ggtcctgccg gtcgattctc cgccgacgga ctgaaggagg ttccgaaagt gctcggcccg
     5821 ggacgctccc ggcggcccga ctgcaggcgc atgatccgag ggtccgcgga gtgtgcgaag
     5881 gtgcggtcac tccccccgca ccttctccag tttctccagg aactcccgcg cctcccgttt
     5941 cgacaatccc ccggatgcca actcatcttc ggacgggcag aaatcagtca gacgcgggcg
     6001 caggatcgcc agctccggcc ccgtgaaatg caccgcctgg agctggtaat ccaggaacgc
     6061 ctcgtacgcc agcgggcaga ccgcctggac catctccgcc atcaccgccg catacgcccg
     6121 gatttcctgc tgggcatgcc ggtcgagccg cagcccgagg aaatgaaaca ggttgtgcag
     6181 gtcgatcttc cagtaccact cggtatacag cgacagcggc aatccgaccc gggccagctc
     6241 ccgggccagc cccgcctcga ccaacgccgc atactcggcg tacgaatccg cctggatctt
     6301 ccggagaaac tcgacaaagc gccgtttcag ctccggcggc acttcctcct ccgcccgccc
     6361 ctgcttgttc accgccgact gaaaccggat ctcctcttcg ggcggaatgt agaactcctc
     6421 tttcatgacc gagtaccgcc ccgagtactc gttgacattg gcggtgcggt ggcggatcca
     6481 ctgccgggcc acgaaaatcg gcagtttcac atggaatttg aactcgacca tctcgaacgg
     6541 cgtcgtgtgg cggtgccgca ggagatagcg gatcagcccc cggtcctccg agactttctt
     6601 ggtccccttg ccgtaggaca cccgcgccgc ctgcacgata gattcatccc cgcccatgta
     6661 atcgatcagc cgcacgaagc cgtggtcgag cacccggaat
//
LOCUS       DAOPOE010000136         6677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12632_length_6677_cov_4.660223, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000136 DAOPOE010000000
VERSION     DAOPOE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_6677_cov_4.660223"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKM94_12955"
     CDS             <1..819
                     /locus_tag="PKM94_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOD67585.1"
                     /translation="LHLHTDCSDGLYAPSEVVRMVREAELAAFALTDHDTLEGYREAR
                     KLLRPGDPELIPGLELSVESAGGELHLLAYLFDPEDAALGEALAAFRERRNRRARQIV
                     ERLNELGISLSYEQVAATAGKAAVGRPHIAQTMHERGLVTAYQSAFDKYIGGGKPAYV
                     PKQNFTPAEAIRAVHAAGGVVVLAHPQIDETYRHLELLAGLGLDGIEMHHPSHQQRHT
                     EQFRHLAERHRLLVTGGSDFHGREGRYGAIGSQRVPAEYLDALRQRAQARKDTL"
     gene            816..3149
                     /locus_tag="PKM94_12960"
     CDS             816..3149
                     /locus_tag="PKM94_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67586.1"
                     /translation="MRHSIALVLAPVLLAGLAAAEPMSRARQEEIIDAYLYITGRAPA
                     PAQALGEGVQLTPDGAPIVKCGMAVAADLLNNRSRIDKELLQAAGIDQSLRPVLRLEE
                     TLVSPDQRFKVHYTRVGDSAVILTDNNGNSIPDYVEATAVILDSVHHNIVGVLGYPAP
                     PRDDFYAGGGDSLYDVYLLDIGAGAYGYTQPEELTGPESMWATSFMALDRNYDGLPDY
                     AGRPLDAIRVTAAHEYFHAVQFALDYFEGTVYLQPTDTVVARYWMEMSSVWMEEKLYD
                     DINDYYNYLPYFFADPRLSLQQFNSYYDLQPYANGICPIFLEQYYDDTLIYAIWKGCE
                     ELGPGPDFLTAANAEIDTFTAGAQQWPQAMREFRIWNWFTGTHADPVPQYVGYEERQA
                     YPELNPARLMRTATYPDTVPANDNPLNPLHNGAAYILLDSTKFVPPDTTYWNCTSVLD
                     SACTDSTEVPQTADWDFFRIGCVSRDTTYWVCNSWSDSVCGGRVRRSVSQDWDFSIVK
                     CSTIVNGIDTSYVELLPRYYKCTLWVDTRCDDSVQVDGQSGYDWITIDSTLRLDLSLG
                     SASPPLPQPWGIAIMYYFVDYPDSVEIDRFTLLDGSVTRLLFPNPDRYSRILVALSPA
                     TYSQDERHYRTYPSIAMRVGYLAAANLVPPEPVPVKPAVWFYPYPNPVNPALPNHTNV
                     AFRFTVPFGEYGEPETTDPEIVVDLFTVAGERVRTLSGAEPVGETAGMYEVHWDLRND
                     AGRDVVSGAYVAVARLYESSARKRVLAESTTKVAVIR"
     gene            3146..3673
                     /locus_tag="PKM94_12965"
     CDS             3146..3673
                     /locus_tag="PKM94_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67587.1"
                     /translation="MISRPLVIARVAVSAALVYVLSWGTSYLPNVNLVFFIVFSAGLL
                     WGAVPGILVGAVGMGLWTMFNPYGPAAAPVAVAQVVGAAGSGVVGALFRRSVGRGGPS
                     WRTTGGLLAASALCTVLFYLPVNLADAWVFQPFWPRFVGGALWAGISLAANAVIFPLL
                     FPAARYLYDRGWAVQ"
     gene            3670..5700
                     /locus_tag="PKM94_12970"
     CDS             3670..5700
                     /locus_tag="PKM94_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67588.1"
                     /translation="MTRRALLAILVLCAGACVGQEAPGDTAGVAPRDTAQVLDTAAAR
                     PADSALAPPADSLSAAERARQSFEERYRQHQEESMRAQRPPSFSPFDSLVDYFTSPRM
                     NMRKAVDRSWYHDAGDYFRLDPSFFVLSHQVVPMRSTVAPFGLMNDRLNLIAYGHRLG
                     PYEHIAEPDGMVDMNAVPTALDNDIFLLAGPVGKLFGGAGAIASLVTRPYRPADRKYH
                     SAFLVNQGGFGYAHVRGRLSKLFSDGRTVDAAVEYRNADGVSPGRESDQYSYYGDFYF
                     PLLGRTGLRTWGWLYDTEGPLAVRPDVGGASLERDRFNRTAEAMFTNDNEAGTRRYEF
                     GYRHERHGLANTGRTAAPLKHRLNWTGHGGFLHGDWLAGRHLVSVSLDGSFLEYDSGG
                     ENIERLSGSFALRLADLSPGWRYALTAEIGVVENYPLLPGGTATVFRDGPHGMVLLAV
                     GFAGRAPSLNELHLPARQSPLYGGSDLYAEEGDERLKRERQLVGSLVLELGTVRNNVN
                     FAVTGGRIFDGIEWAPEPAAAALVFRPVNTTLDFVTATVTPRLSLGEFAAFRGGVSYH
                     WLDYEKIEDRAYAPEYQAFSGLELHVAWPQRRLNLYAYGEVVYTGPYQGYVEQNLGED
                     PVFNGKLSFMLNDDYRMHFVFENVLSRAYSQRDYLVFPGRYFYLGFEWSFLD"
     gene            5843..6382
                     /locus_tag="PKM94_12975"
     CDS             5843..6382
                     /locus_tag="PKM94_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOD67589.1"
                     /translation="MLRRDDAVLAVVDVQGRLATLMHDKETFYENVARMIKGAKVLGL
                     PILWTEQLPDKLGDTLPQIAEHLAGEARLVKRTFSCCGDENFLARLKATGRRQVLLTG
                     METHVCVYQTAMDLLAGGYEVYLVSDAVSSRTAANRAVGIEAIREAGGRITSVEMALF
                     EMLVVAQGDQFKQVIQIVK"
     gene            6442..>6677
                     /locus_tag="PKM94_12980"
     CDS             6442..>6677
                     /locus_tag="PKM94_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67590.1"
                     /translation="MRTMRMLMVAGAVVCLAGGMARADAYIKNVTETEAMQLGGQTRP
                     ASSDTTEIWVSPSRAAMVMGGGQKFIVDAKKQTL"
BASE COUNT         1079 a         2236 c         2201 g         1161 t
ORIGIN      
        1 ctccacctgc acaccgactg ctcggacggc ctctacgcgc cctccgaggt ggtgcgcatg
       61 gtgcgagagg ccgagctcgc ggccttcgcc ctcaccgacc acgacaccct ggaggggtac
      121 cgcgaggcgc gcaaactcct ccggcccggc gacccggaac tgatcccggg gctggagctg
      181 tcagtcgaga gcgcgggggg ggagttgcac cttctggcgt acctgttcga cccggaggac
      241 gccgcgctcg gcgaggcgct cgccgcgttc cgcgagcgcc ggaaccggcg ggcgaggcag
      301 atcgtagaaa ggctgaacga actggggatc tccctgagct acgagcaagt ggccgcaacg
      361 gccggaaagg cggcagtggg ccgtccgcat atcgcccaga cgatgcacga gcgggggctg
      421 gtgacggcct accagagcgc tttcgacaag tacatcggcg gcggcaaacc cgcttatgtc
      481 ccgaagcaga acttcacgcc ggcggaggcg atccgcgccg ttcacgcggc cggcggcgtc
      541 gtcgttctgg cccacccgca aattgatgaa acctaccggc acctcgagct gctggccggg
      601 ctcggcctgg acggcatcga aatgcaccac ccgtcgcacc agcagcggca caccgagcag
      661 ttccgccacc tggccgaacg ccaccgtctg ctcgtcaccg gcggctccga tttccacggg
      721 cgcgaagggc gctacggggc gatcggatcg cagcgcgtgc cggccgagta tctcgatgcg
      781 ctccggcagc gggcgcaggc acgaaaggat accctgtgag acacagcata gccctggtcc
      841 tggcgccggt cctcctggcc ggcctggcgg ccgccgagcc gatgtcgcgc gcgcgccagg
      901 aggaaatcat cgacgcctac ctctatatca ccgggcgggc gcccgctccg gcccaggcgc
      961 tcggcgaggg ggtgcagttg accccggacg gcgcgccgat cgtgaagtgc gggatggcgg
     1021 tcgccgccga tttgctcaac aaccgcagcc ggatcgacaa ggaactcctg caggcggccg
     1081 gcatcgacca gtcgctgcgc cccgtcctcc ggctcgagga gacgctcgtc agccccgatc
     1141 agcggttcaa agtccactac acccgggtcg gggactcggc ggtcatcctg accgacaaca
     1201 acggcaactc cattccggac tatgtcgagg ccacggccgt cattctcgac agcgtccacc
     1261 acaacatcgt cggcgtcctg ggctacccgg cgccgccgcg ggacgacttc tacgccggcg
     1321 gcggggactc gctctacgac gtctacctgc tcgacatcgg ggcgggcgcc tacggataca
     1381 cgcagcccga ggagttgacc ggtcccgagt ccatgtgggc gacctcgttc atggcgctgg
     1441 atcgcaacta tgacggcctc cccgactacg ccgggcggcc cctcgatgcg atccgggtca
     1501 cggccgcaca cgagtatttc cacgccgtgc agttcgctct cgattatttt gaggggactg
     1561 tctaccttca gccgaccgac acggtcgtgg cgcggtactg gatggagatg tcgtcggtgt
     1621 ggatggagga gaagctctac gacgacatca acgactacta caactacctg ccctacttct
     1681 tcgccgaccc ccggctgtcg ctgcagcagt tcaactcgta ctacgatctg cagccgtatg
     1741 cgaacggcat ctgcccgatt ttcctcgagc agtattacga cgacacgttg atctacgcga
     1801 tctggaaagg gtgcgaggag ctggggccgg gcccagattt tctgacggcg gccaacgccg
     1861 agatcgacac gttcacggcc ggcgcccagc agtggccgca ggcgatgcgc gagttccgca
     1921 tctggaactg gttcaccgga acgcacgcgg acccggttcc ccagtatgtc ggctacgagg
     1981 aacggcaggc ctaccccgag ctgaatccgg ccaggctcat gcgcaccgcc acctatccgg
     2041 acacggtgcc cgcgaatgac aacccgctca acccgctgca caacggcgcg gcgtatattc
     2101 tccttgacag caccaagttc gtgccgcccg atacgaccta ctggaactgc accagcgtgc
     2161 tcgattctgc ctgcaccgat tccaccgaag tgccgcagac ggccgactgg gatttcttcc
     2221 gcatcggctg cgtctcccgc gacacgacct actgggtctg caactcctgg tccgattcgg
     2281 tgtgcggcgg gcgcgtgcgc cggagcgttt cccaggattg ggacttctcg atcgtaaagt
     2341 gttcgacgat tgtgaacgga atcgacacca gttacgtcga gctgctgccg cgctactaca
     2401 agtgcacgct ctgggtcgac acgcggtgcg acgattcggt tcaggtcgac gggcagtcgg
     2461 ggtacgactg gatcacgatc gacagcacgc tccgtctcga cctctcgctc ggttcggcgt
     2521 cgccgccgct gccgcagccg tggggcatcg ccatcatgta ctacttcgtg gactacccgg
     2581 attcagtgga aattgatcgg ttcacgcttc tggacggcag tgtgacgcgc ctgttgtttc
     2641 ccaaccccga ccgctacagc cgcattctgg tcgccctctc gccggcgacc tactcgcagg
     2701 acgaacggca ctatcgcacc tatccgagca tcgccatgcg cgtgggctac ctggccgccg
     2761 ctaatctcgt cccgccggag ccggtgccgg tcaaaccggc ggtgtggttc tacccgtatc
     2821 ccaacccggt caaccccgct ctgccgaatc acaccaacgt cgccttccgc ttcaccgtgc
     2881 cgttcggcga gtacggcgaa ccggagacga ccgaccccga aatcgtggtg gacctgttca
     2941 cggtcgccgg ggagagggtg cggacgctgt cgggagcgga gccggtcggg gagaccgccg
     3001 gcatgtacga ggtgcactgg gatctgcgca acgacgccgg ccgggacgtc gtctccggcg
     3061 cctacgtcgc ggtcgcgcgc ctttacgaat cgagtgcgcg caagagggtg ctggcggaga
     3121 gcacgacaaa ggttgccgtt atccgatgat ttcgcggccg ctggtgatcg cgcgggtggc
     3181 cgtgagtgcg gcgctcgtgt acgtgctgtc gtgggggacc tcctacctcc cgaatgtcaa
     3241 cctcgtgttc ttcattgtct tctccgccgg gctgctctgg ggggcggttc cgggaattct
     3301 cgtgggcgca gtgggcatgg gcttgtggac gatgttcaat ccgtacggtc cggccgcggc
     3361 gccggtagcc gtggcccagg tggtcggagc ggccggctcg ggagtggtgg gagcgctctt
     3421 ccggcggtca gtcgggcggg gcggcccgtc gtggcggacg accggcgggc tgctggccgc
     3481 gtcggcgctc tgcacggttc ttttctacct gccggtcaac ctcgccgacg cctgggtctt
     3541 ccagcccttc tggccgcgtt tcgtcggcgg cgccctgtgg gcgggcatct cgctggccgc
     3601 caacgccgtg attttcccgt tgcttttccc ggccgcccgc tacttatatg accgaggatg
     3661 ggccgttcag tgacacgccg cgctctcctg gccattctgg tgctctgcgc cggcgcctgt
     3721 gtcgggcagg aggctcccgg cgataccgcc ggggtcgccc cccgtgacac cgcccaggtt
     3781 ctcgataccg ccgccgcccg ccccgccgac tccgcgctcg cgccgccggc cgattccctc
     3841 tccgccgccg agcgggcccg gcagtcgttc gaggagcgct accgccagca ccaggaagaa
     3901 tcgatgcggg cgcagcgccc gccgtctttc tctcccttcg actcgcttgt cgactatttc
     3961 accagcccgc ggatgaacat gcgcaaggcg gtggaccgct cctggtacca tgatgccggc
     4021 gactacttcc gcctggatcc gtccttcttc gtgctcagcc accaggttgt tccgatgcgc
     4081 tcgaccgtgg cgccgttcgg cctcatgaat gaccggctca atctcatcgc ctatggccac
     4141 cgtctcggcc cgtatgagca cattgccgag ccggacggca tggtcgacat gaacgccgtc
     4201 cccacggcgc tcgacaacga catcttcctg ttggccggac cggtcgggaa gctgttcggc
     4261 ggtgcgggcg cgatcgcctc gctggtcacc cggccctacc ggccggcgga ccggaagtac
     4321 cacagcgctt tcctggtcaa ccagggcggg ttcgggtacg cccacgtgcg cggacgcctg
     4381 agcaagctgt tcagcgacgg ccggacggtc gatgcggcgg tcgagtaccg caacgccgac
     4441 ggcgtgtcgc ccgggcgcga atcggaccag tacagctact acggcgattt ctatttcccg
     4501 ctcctcggtc gcaccggcct gcgcacctgg gggtggctct acgacaccga gggtccgctg
     4561 gcggtgcgcc ccgatgtcgg cggagcatcg ctcgaacggg atcggttcaa ccggaccgcg
     4621 gaggcgatgt tcaccaacga caacgaggcc ggtacccggc ggtatgagtt cgggtatcgg
     4681 cacgagcgcc acggtctggc caacaccggc cggacggcgg ccccgctcaa gcaccgcctc
     4741 aactggaccg gccacggcgg gttcctgcac ggcgactggc tggccggccg ccatctcgtc
     4801 tcggtttcgc tcgatggttc ctttctcgag tacgactccg gcggcgagaa catcgagcgg
     4861 ctgagcggtt ccttcgctct ccgcctggcc gatctctcgc ccgggtggcg gtatgctctc
     4921 accgccgaga tcggcgtcgt cgaaaattac ccgcttctgc cggggggaac ggcgacggtg
     4981 ttccgggacg gtcctcacgg gatggtgctg ctggcggtcg ggttcgccgg ccgcgcgccc
     5041 agcctgaacg agctgcatct tccggcccgg cagtcgcccc tctacggcgg gtctgatctc
     5101 tacgcggagg agggggacga gcgtttgaag cgggagcggc agctcgtggg ttcgttggtg
     5161 ctcgagctcg gcaccgtccg caataacgtc aatttcgcgg tgaccggcgg tcgcatcttc
     5221 gacggcatcg agtgggcgcc cgaaccggcc gccgccgcgc tcgttttccg gccggtcaac
     5281 acgaccctcg atttcgtcac cgcgacagtc accccgcgcc tcagcctggg tgagttcgcc
     5341 gcctttcgcg gcggcgtgtc ctaccactgg ctggactacg agaagatcga ggaccgggcg
     5401 tacgcacccg aataccaggc cttctccggg ctggaactcc acgtggcttg gccgcagcgg
     5461 aggctcaacc tctacgccta cggggaggtc gtctacaccg ggccctatca ggggtacgtg
     5521 gagcagaacc tgggcgaaga tccggttttc aacgggaagc tctcgttcat gctcaacgac
     5581 gactaccgca tgcattttgt cttcgagaat gtcctctcgc gtgcgtacag ccagcgggac
     5641 tacctggttt ttccgggacg gtacttctat ttgggatttg aatggtcctt tttggactga
     5701 ccgtgcgccc ggccggcggc gcgtgaaacc tcggcgcaga aggtgcttgc ccgaccgccg
     5761 gcggcgccta tcttgcgggg tcgcggtcag ccgaaagcgg ccgcagaacc attgaatcaa
     5821 cgggtggtgt gtgaagagaa gtatgctcag gagagacgac gcggtgctcg cggtggtgga
     5881 cgtgcagggg agactggcga ccctcatgca tgacaaggag acgttttacg aaaacgtcgc
     5941 gcgaatgatc aaaggagcga aggtgctcgg gctgccgatc ctctggaccg agcagctccc
     6001 cgacaaactc ggcgacactc tcccgcagat cgccgagcat ctggccggcg aggcccggct
     6061 ggtcaagcgg accttttcgt gctgcggcga cgagaacttc ctggcccgcc tcaaagcgac
     6121 cggccgtcgg caggtgctcc tcaccggtat ggagacgcac gtgtgcgtgt accagacggc
     6181 gatggatctg ctggcgggcg gttacgaggt ctacctggtg agcgacgcgg tctcctcgcg
     6241 caccgcggcc aaccgggccg tcgggatcga ggcgatccgg gaggccggcg ggcgcatcac
     6301 gagcgtagaa atggccctct tcgaaatgct cgtggtcgcg cagggggatc aattcaaaca
     6361 ggtcatacaa atcgtaaagt gatcggagcc tccggcgggc tgcgacagga cgccgaaggg
     6421 cggttcaagc aaaggagtaa catgcggaca atgcggatgc tgatggtggc cggggcggtc
     6481 gtgtgcctgg ccggcgggat ggcgcgggcc gacgcgtaca tcaagaacgt caccgaaacc
     6541 gaggccatgc aactgggcgg gcagacccgt ccggcatcct cggacacgac cgaaatctgg
     6601 gtgtcgccgt cccgggccgc catggtcatg gggggcggcc agaagttcat tgtcgatgcg
     6661 aaaaagcaga ccctttg
//
LOCUS       DAOPOE010000137         6676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_12634_length_6676_cov_5.410059, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000137 DAOPOE010000000
VERSION     DAOPOE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12634_length_6676_cov_5.410059"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..2265
                     /locus_tag="PKM94_12985"
     CDS             28..2265
                     /locus_tag="PKM94_12985"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOD67591.1"
                     /translation="MRLRFHTRLGAGDYLKFRGVRFGRRGGARSLMTAVAIAAALSGG
                     AAADEGMWPLYELDRLDWDSLQARGLRLSREQMYDTARPDVADACINLGGGSASFVSP
                     KGLIITNHHVAFGAIQRASTVEHNYLRDGFYAPTPAEEIRAVGTSVYVTLAEADVSDR
                     VLAAVPEELDGLARYEALDRITKEIVREAEQGRDDIQAEVTPIYGGTKYTLTTFLRIR
                     DVRIVHAPPMAIGNYGDEIDNWIWPRHVGDYSFLRAYVGPDGKPAEYSRDNVPYEPQA
                     WLPVSAQGVKEGDFTMMIGYPGKTQRYTVARAIAEAVNFTYPRSIDRSLQLLAKIDSA
                     GAENPGIALRMASTVQGISNRMKKTQGLVAGLARDSVVARTAAAEAELTVFLRGDSAR
                     WERFGGLIPAFDSLYDAHAAARERREVLGMVWYVDHLSLARQVYRWAEQREKPDPERE
                     SGYQDRDSLRVLRRLEQAQINLVPAMDRVFMRYWLEQALALPDGQRLAAVDSLFAGGP
                     ERDRFLDRLLDRMFAQTKVGQEQARREMFRMSTADLRRLRDPFIDLAAALGPEFERMQ
                     TEERVFASAAGRLERQYMRALQAWKGDRLYPDANGTKRFSFGEVRRYSPRDAVTHDYI
                     TSLTGVMEKETGAEPFIVPAELKQVWRRRDFWPYVDAHLNDVPVDFITTNDGTNGNSG
                     SPVLNGRGELIGLDFDTNIESVHKDFCYNRNLSRAVVADVRYILFLLDKVYGYRALLD
                     ELTVR"
     gene            2380..3234
                     /locus_tag="PKM94_12990"
     CDS             2380..3234
                     /locus_tag="PKM94_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67592.1"
                     /translation="MRRTWVLVLVALAAGLTAGCFDYEEQMVIDKDGSGKVSFHYAVN
                     KEYLQQFEDFAKSMAALAGEDSTEVNLGFAELSEDSIRQAIAQTTEGVELVSYRASED
                     EANKIWDMTFTFADLNAAAGVSNLLTPDDEEGDATGLAEAGLSGITFERQPDGTWLYR
                     RPLESGSDEEEPGMEEEGASADEAEEGEATEPEEAGEAPADETVEEAAASDDAGEAGT
                     MEPADVDSQQNEFAKAFAGHKVRFIVTFPGKVIESNASTVDGNMAIWEYSLDAIPALQ
                     EMRAVVAD"
     gene            complement(3297..4376)
                     /gene="rsgA"
                     /locus_tag="PKM94_12995"
     CDS             complement(3297..4376)
                     /gene="rsgA"
                     /locus_tag="PKM94_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOD67593.1"
                     /translation="MDLTEFGFDDRWGALMAPYAAAGLVPARVVRQHRERSIVIAAAG
                     ELTGEVAGRFRRDSAGRSDYPVVGDWVAAEIAGDRLAVIHAVLPRRSAFTRKVAGNLT
                     EAQVVAANIDTVFLVTGLDGNFSLRRIERYLTSAWDSGASPVIVLNKADLCGDPAEVL
                     AGVEAVALGAPVVALSALDGSNVEALRPYLVPGRTAALLGSSGVGKSTLINRLLGEER
                     LPTREISDRAGRGRHTTTHRELVQLPDGALLIDTPGMRELQLWADEDSLERAFDDIEA
                     LAARCRFADCRHEAEPGCAVRAAVDAGSLDPARLESYFKQRRELAFLGLKMDERSRRQ
                     ADKSRGRRFAQMRKEIKQRKPNFDK"
     gene            4932..6398
                     /locus_tag="PKM94_13000"
     CDS             4932..6398
                     /locus_tag="PKM94_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOD67594.1"
                     /translation="MSRHTEYDAVVVGSGPNGLAAAIALAETGLSAAVYERAAAPGGG
                     LRSSELTLPGFVHDVCSAVHPLAAGSPFLARLPLQAYGLDWVHPPIAYAHPLDDGSAA
                     AVWRSMEDTAGHLAGDRAGYLRLMAPLVRGWDKLFPEALAPLHFPRHPAAMARLGMRA
                     LRSARGVADAHFRSARGRALFAGAAAHGVLPLDRPVSAAAGLVLGAAAHTAGWPMPRG
                     GAQRLANALAAHARTLGVEISLNREIRSLEDLPPARAVLLDLSPRQVLDIAGTRLPEA
                     YRKRLAQYRHGPGVCKVDWALREPIPFTAPECRRAGVVHVGGTFAEVAEAERLVWAGK
                     HPERPFVLLAQPTLFDLSRAPAGRHTAWGYCHVPNGSTIDMTGAIEAQIERFAPGFRE
                     VIIGRHTLHAADMERYNPNYVGGDITGGVLDARQLFTRPAGWRAPYRTPLAGVYICSA
                     STPPGGGVHGMCGYHAAILVLKDLFGGGRPGRADRRRL"
     gene            6503..>6676
                     /locus_tag="PKM94_13005"
     CDS             6503..>6676
                     /locus_tag="PKM94_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67595.1"
                     /translation="MGVLAIDNLSLSLNDKPILQDLNIDFWEGHVHAVVGPNGAGKST
                     LAATIMGLAGYDGY"
BASE COUNT         1129 a         2067 c         2406 g         1074 t
ORIGIN      
        1 gctggaaacc tgagcaggag ttggcgtatg cgtcttcgtt tccacaccag gctcggcgcg
       61 ggggattacc tgaagttccg aggagttcgc ttcggccgtc gcgggggcgc tcgcagcctg
      121 atgacggcag tcgcgatcgc ggccgctctg agcggcgggg ccgccgccga cgaggggatg
      181 tggccgctgt atgagctcga ccgccttgat tgggacagcc tccaggcccg ggggctccgg
      241 ttgtcgcggg agcagatgta cgacactgcg cggccggatg ttgcggatgc ctgcatcaac
      301 ctcgggggcg ggagcgcctc cttcgtctcg ccgaaagggc tgatcatcac aaaccaccac
      361 gtggctttcg gggcgatcca gcgcgcctcg acagtcgagc acaactacct tcgggacggc
      421 ttctacgctc ccacgcccgc ggaggagatc cgcgcggtgg gaacgagcgt gtatgtcacg
      481 ctggcggagg cggacgtgag cgaccgggtg ctggcggcgg tgccggagga actcgacggc
      541 ctggcgcgct atgaggcgct cgaccggatt acgaaagaga ttgtccggga agccgagcag
      601 ggccgggacg acatccaggc ggaggtgacc ccgatctacg gcgggacgaa atacacgctg
      661 acaaccttcc tgcgcatccg cgacgtccgc attgtgcacg cgccgccgat ggcgatcggg
      721 aactacggcg acgagatcga caactggatc tggccccgcc acgtgggaga ctattccttc
      781 ctccgggcat acgtggggcc cgatggaaag ccggccgagt attcccggga caatgtcccg
      841 tacgagccgc aggcgtggct gccggtgtcg gcacagggag tcaaggaggg ggatttcacg
      901 atgatgatcg gttatccggg gaagacgcag cggtacacgg tggcgcgcgc catcgccgag
      961 gcggtgaact tcacctaccc gcggtcgatc gaccggtccc tgcagctgtt ggcgaaaatc
     1021 gattcggcgg gagcggagaa tcccgggatc gctctgcgca tggcctcgac ggtgcagggg
     1081 atcagcaacc ggatgaagaa gacgcagggg ctggtggccg gtctggccag agacagcgtc
     1141 gtggccagga cggcggccgc cgaggccgaa ttgacggttt tcctgcgcgg cgattccgcg
     1201 cgctgggagc gtttcggcgg tttgatcccg gcattcgaca gtctctacga tgcccacgcc
     1261 gctgcgcgcg agcggcggga agtgctcggc atggtctggt atgtcgatca cctctctctg
     1321 gcccggcagg tgtatcgatg ggcggagcag cgggagaaac cggaccccga gcgcgagagc
     1381 ggttaccagg accgggattc gctccgcgtg ctgcggcgcc tggagcaggc gcagatcaat
     1441 ctcgtcccgg cgatggaccg cgtgtttatg cgctactggc tcgagcaggc gctggcgctg
     1501 ccggacgggc agcggctggc cgcggtcgac agcctgttcg ccggcgggcc ggagcgcgac
     1561 cggttcctgg accgactgct cgaccggatg ttcgcgcaga ccaaagtcgg gcaggagcag
     1621 gcgcggcggg agatgtttcg catgtcgacc gcggacctgc ggcgcctgcg cgatccgttc
     1681 atcgacctcg cggcggcgct cggacccgag ttcgagcgga tgcagaccga agagcgggtc
     1741 ttcgcgagcg cggcagggcg gctggaacgc caatatatgc gggcgctgca agcgtggaaa
     1801 ggagaccgcc tctacccgga tgccaacgga accaagcggt tcagcttcgg cgaggtgcgc
     1861 cgctactcgc cgcgcgacgc ggtcacccac gattacatta cgagtctgac cggggtgatg
     1921 gagaaagaga ccggagcaga accgtttatc gtgccggccg aactgaaaca agtctggcgg
     1981 cggcgcgatt tctggccgta tgtcgacgcc cacctgaacg acgtgccggt cgatttcatc
     2041 acgaccaacg acggcaccaa cggcaattcc ggcagtccgg tgctgaacgg ccgaggggaa
     2101 ttgatcggcc tggatttcga caccaatatc gagagcgtgc acaaggattt ctgctacaac
     2161 cgcaacctct cgcgggcggt ggtggcggat gtccgctaca tcctgtttct tctggacaag
     2221 gtgtatggct accgcgcgct tctggatgaa ctgacggtcc ggtgaaatcc gcggcgcgcc
     2281 gcaaccgggc gccgagttga tgcttgacag gcgacccggc ggccacgatt aatgtccggt
     2341 agaaaaatca gatccgaatc aactatcacg aggtgtatca tgaggcgcac ctgggtgctg
     2401 gtgctggtgg cgctcgcggc cgggctgacc gcgggttgtt ttgattacga agagcagatg
     2461 gtgatcgaca aggacgggtc ggggaaagtc tccttccact acgccgtcaa caaagagtac
     2521 ctgcagcagt tcgaggattt cgcaaagtcg atggctgcgc tcgcgggcga ggattcgacc
     2581 gaggtaaacc tcggattcgc cgagctgtcg gaggattcga tccggcaggc gatcgcccaa
     2641 acaaccgagg gcgtggaact ggtctcgtac cgggcgtcgg aggatgaggc caacaaaatc
     2701 tgggacatga ctttcacgtt cgccgatctc aacgccgcgg ccggagtctc caacttgctc
     2761 accccggatg acgaagaggg agacgcgacc ggactcgccg aggccggcct cagcgggatt
     2821 acgttcgaac gtcaacccga cggcacctgg ctgtaccgac ggccgctgga gtcggggagc
     2881 gacgaggagg aaccgggaat ggaggaggaa ggggcaagtg cggacgaggc cgaggaggga
     2941 gaggcgactg agccggagga ggccggagaa gcgcctgcgg acgagacggt cgaggaagct
     3001 gcggcctcgg acgatgcggg agaggccggg actatggaac cggccgacgt cgacagccag
     3061 cagaacgagt ttgccaaagc ctttgccggg cacaaggtca gattcatcgt caccttccca
     3121 ggcaaagtca tcgagtcaaa cgccagtacc gtcgacggaa acatggccat ctgggaatac
     3181 agcctcgatg ccatcccggc gctgcaggaa atgcgagcgg tggtggcgga ttgacggcgc
     3241 ggccggccgc gaaacgatac ccggcggaca gcgagtccgc cgggtctttt ttttgcctac
     3301 ttgtcaaagt tcggtttgcg ctgcttgatc tcctttctca tctgcgcgaa acgccggccc
     3361 cggcttttgt ccgcctgccg gcgggagcgt tcgtccatct tgagcccgag gaacgcgagc
     3421 tcccggcgct gcttgaaata gctctcgagc cgcgccgggt cgagggatcc ggcgtcgacg
     3481 gccgcgcgca ccgcgcagcc gggctcggct tcgtggcggc agtcggcgaa gcggcagcgg
     3541 gcggcgagcg cctcgatgtc gtcgaaagcg cgttcgaggc tgtcctcatc ggcccacaac
     3601 tgcagctcgc gcatgccggg ggtgtcgatg aggagcgcgc cgtcgggaag ctgcaccagt
     3661 tcccggtggg tggtggtgtg gcggccccgg ccggcgcggt cgctgatctc gcgggtgggg
     3721 agacgctcct cgccgaggag acggttgatg agggtcgact tccccacgcc cgaggacccg
     3781 agcaatgcgg cggtgcggcc gggaaccagg tatggccgca gggcctctac gttgctcccg
     3841 tcgagggcgc tcaacgcaac caccggcgcc ccgagcgcga ccgcctccac gccggccagt
     3901 acttccgccg ggtcgccgca caggtcggcc ttgttgagca cgatcaccgg agaggcgccg
     3961 ctgtcccagg cgctcgtcag gtagcgctcg atgcggcgga ggctgaaatt gccgtcgaga
     4021 ccggtcacga gaaagacggt gtcgatgttg gcggcgacca cctgcgcctc ggtcaggttc
     4081 ccggccacct tgcgcgtgaa agcgctgcgg cgggggagca cggcgtgaat gaccgcgagc
     4141 cggtcccccg cgatctccgc ggccacccag tcgccgacca ccgggtagtc gctccggccg
     4201 gccgagtcgc ggcgaaagcg gccggcgacc tcgccggtca attcgcccgc cgcggcgatc
     4261 acaatgctgc gctcgcgatg ttgtcggacc acgcgggccg ggaccagtcc cgctgccgcg
     4321 tacggggcca tcagcgcgcc ccagcgatca tcaaatccga attcagtcag gtccattata
     4381 tgctgctctc cgacacattg tccgaatttc cccggggcgc caacggcgaa gacgtatgcc
     4441 ggtgcggcgc cctcaggccg tacggccgtc ggggttgact gttggggtga gagcggggaa
     4501 tacgacctca cgccttcgga ggagggggcg aagaccgggg attcgggaag gacagagcgg
     4561 cgatacgccg gcggcgcatc gcttcaggaa gagatggcgg cgctagcggc ccagtcctcg
     4621 agcgtgtgag ttccccgcgg tgcctacgac aatggccaac atcaaaaact cctttctcgg
     4681 cgggcagcgg cgcccgcggt ggcggtgggg tcgccggccg tccatgaacg agagccggcg
     4741 cgctaattcc ttattcccta cgatacatga tttcggaaag ttgtaaagaa aaatgaggag
     4801 gcgagggggc ccgatcctcc gcccgtggtt ctccgagagc gaaccgaaca gggggaggcg
     4861 tgttgtagtg acaggcgcgg ccggttgtgc gcgacacggg ggcgttttcc cgacggcgcc
     4921 gaggcgacgt gatgagcagg catacggagt atgatgcagt ggtcgtggga tccggcccca
     4981 acggtctggc ggccgcgatt gccctggctg agaccgggct gagcgcggcc gtctacgaac
     5041 gggccgccgc cccgggcggg ggactccgct cgtccgagct gacgcttccg gggttcgtgc
     5101 acgacgtctg ctcggctgtc catccgctcg cggccgggtc gccgttcctg gcgcggctgc
     5161 cgctgcaggc ctacggactc gactgggtgc atccgccgat cgcctacgcg cacccgctgg
     5221 acgacggctc ggcggcggcc gtgtggcgtt caatggagga tacggccggg catctggcgg
     5281 gggaccgcgc cgggtacctt cgcctgatgg cgccgctggt gcgggggtgg gacaagctgt
     5341 tcccggaggc gctggcgccc ctgcattttc cccggcatcc cgccgcgatg gcccggttgg
     5401 ggatgcgggc gttgcggtcg gcgcgggggg tggcggacgc acactttcgc tccgcgcgcg
     5461 ggcgggcctt gttcgctggg gcggcggcgc acggggtgct gccgctggac cggccggtct
     5521 ccgccgcggc cgggctcgtc ctgggggcgg ccgcccacac ggcgggctgg ccgatgcccc
     5581 gcggcggcgc tcagcggctg gccaacgctc tcgcggcgca cgcccgcacg ctcggagtcg
     5641 agatctccct caatcgcgag attcgcagcc tggaggacct tcccccggcc cgagcggtcc
     5701 tgctcgacct ctcgccccgg caggtgctcg atattgcggg aacccggctg ccggaggctt
     5761 accgcaaacg actcgcccag taccggcatg gaccgggggt gtgcaaggtg gattgggcac
     5821 tgcgcgaacc gattcccttc accgcgccgg aatgtcgccg ggcaggtgtc gttcacgtgg
     5881 gcgggacctt cgcggaggtg gcggaggcgg agcggctggt atgggccggg aaacatccgg
     5941 agaggccgtt tgtgctgctg gcgcagccga ccctgttcga tctctcacgg gcgcccgcgg
     6001 gccgacacac tgcttgggga tactgccatg tcccgaacgg ctcgacaatc gacatgaccg
     6061 gggcgatcga agcgcagatt gagcgcttcg cacccgggtt tcgcgaagta atcatcggcc
     6121 ggcataccct gcacgcggcg gacatggagc ggtataaccc gaactatgtc ggcggcgata
     6181 tcaccggcgg cgtactcgat gcgcggcagt tgttcacccg gcccgcggga tggcgggcgc
     6241 cttaccggac gccgctcgcg ggggtgtaca tctgctcggc ctcgacgccg cccggcggcg
     6301 gagtgcacgg catgtgcggg tatcatgcgg ccatcctggt tttgaaagac ctgttcggcg
     6361 gggggcggcc gggtcgggcg gaccggcggc gattgtgaaa aatttctcgt gcatttgccg
     6421 gcaaacgacc ctttattgta tcggaacagt caaccatcga acccgcggac gcgggtcgga
     6481 gacgtcgcga aaggacccgc ccatgggtgt gctggcgatc gacaacctga gcctgtcgct
     6541 gaacgacaag ccgattctcc aggacctgaa cattgacttc tgggaggggc atgtccacgc
     6601 agtggtcgga ccgaacgggg ctggcaaatc cacgctggcc gcaaccatca tgggcctggc
     6661 ggggtatgac ggctac
//
LOCUS       DAOPOE010000138         6463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_13110_length_6463_cov_5.390918, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000138 DAOPOE010000000
VERSION     DAOPOE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13110_length_6463_cov_5.390918"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKM94_13010"
     CDS             <1..913
                     /locus_tag="PKM94_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD67596.1"
                     /translation="GASAGFGIMLSVHNSLCCEIIKLFGSETLKQKYLPAMAAGECIG
                     AYCITEPDAGTDVASLKTTAADGGDGWVLNGTKSYVTNGSFAGLFVVFAKTDPDAGHK
                     GISCFLVPAGTPGLMIGKAERKCGIKASDTREVSFSDVRIPKDHILGAPGQGFRMAVT
                     ILNSGRVGVAFQSIGIAQAALNEALKYAKERKQFNQPIANFQAIQFKLADMALKIDAG
                     RLLAYRAAQLKDAGEPCHREASMAKLFCSEMANEVANEAVQIHGGYGYIKEYAVERYF
                     RDARVTMLYEGTSEAQRMVISRDLLRE"
     gene            918..2066
                     /locus_tag="PKM94_13015"
     CDS             918..2066
                     /locus_tag="PKM94_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD67597.1"
                     /translation="MLEKRHLEYREQIRAFMEDKIKPLAHTLDVEQRVAHEHMKPLTD
                     MGLMSMLIPEKYGGRPTDTVTYTIAVEEASRVCGSTGILLAAHNSLGCYPILAFGTEA
                     QRQRYLPRAAQGEILAFGLSEPDAGSDAGGTRTMAVKKADHWLINGSKCWITSATRAF
                     ATIASARTSDDPENKEITCFILEKDWPGYAVGKKENKLGLRGSDTAFLHFDDLKAPLD
                     AQLGIVGDGFKQMLKTLDGGRISIAAMALGLAQGAFDEALRYSASRVQFGKPISYNQA
                     IQHKLADMATQLEAARLLVYESARMKDAGAPFGHYSAMAKLYASEIGVRVCADAMGIL
                     GAIGYYTGPYPVERMWRDVKLCTIGEGTSEIQKLVIARYLLKQVQEKS"
     gene            2220..3158
                     /locus_tag="PKM94_13020"
     CDS             2220..3158
                     /locus_tag="PKM94_13020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOD67598.1"
                     /translation="MTPTDFIWMNGKLIPWKDATIHICSHVIHYGSSVFEGMRVYKTP
                     KGPAAFRLREHTQRLLNSAKIYRMTVPYTVEELDRAILETVAANRLEACYVRPIVYRG
                     YGTLGVDPTKCPIDVAIAVWPWGQYLGEEALAKGVSVCVSSWHRLAPNTMPAMAKAGA
                     NYMNSQLIKLEALSHGYVEGIALDTSGNVSEGSGENVFLVRNGVLVTPTFAASILPGI
                     TRRSVIRIAEDMGIKVIEENVPREALYIADEVFLTGSAAEITPISRIDNIVIGEGNAG
                     PITRKIQKAFFDILEGRAEDRYGWLTLVPKPKNAAV"
     gene            3163..3633
                     /gene="rpiB"
                     /locus_tag="PKM94_13025"
     CDS             3163..3633
                     /gene="rpiB"
                     /locus_tag="PKM94_13025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOD67599.1"
                     /translation="MKFAIGSDHAGFEMKERVKEILRELGQEVEDVGTHSKESCDYPD
                     FGVRVARMVREHRAERGVLVCWTGNGMTIAANKVPGVRAGLALNADMAYLTRRHNDAN
                     VLTLAQKYTPDSELREILRRFIETEFEGGRHERRLNKIRAVEPGNGTPSPPQNA"
     gene            3686..4198
                     /locus_tag="PKM94_13030"
     CDS             3686..4198
                     /locus_tag="PKM94_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67600.1"
                     /translation="MKHRIMFVRAAVLAVLVTVGPAGDSGAQERFSRFRLVTQDGGVV
                     EGFRGRLEGQVFRGTTRAGDQFEIACDRLIQLEQYRGRRTGAGLALGILSGLSVGVVY
                     MLLNDDTSDEAGTIPRTVAVFGGLAAAGGVVGAAIGGATTLWDPVPLGRGEIGGAREL
                     EGTAAVVFRF"
     gene            complement(4206..5042)
                     /locus_tag="PKM94_13035"
     CDS             complement(4206..5042)
                     /locus_tag="PKM94_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67601.1"
                     /translation="MIHPVSRELARSLELIEIAAMVDVFAAAPDKIARRLGARVIECS
                     PMTGVLVPGLDVLVFNRVIGFGLQPAIDPVDVDRCLDHYRAAGVKRFFIQTCPVGAPE
                     NARQVLGARGLRWHNNWVKLYRDTSPPPASELSVTVREIGRDQALRFGELVCTAFEWG
                     DAIVEWIATLVGRPRWRIYGAFDGWRMIAGAALFVHGDFGWLDFAATDKDYRASGAQS
                     ALLHRRIAEAAALRLRTLVLETAEQTAERGAPSFRNVQRFDFEVAYVRPNFLWSREPA
                     PE"
     gene            5224..>6463
                     /gene="glyA"
                     /locus_tag="PKM94_13040"
     CDS             5224..>6463
                     /gene="glyA"
                     /locus_tag="PKM94_13040"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOD67602.1"
                     /translation="MSYLKQTDPEIYEAMQRETDRQAHKLELIASENFVSLAVMEAAG
                     GVMTNKYAEGYPGKRYYGGCEWVDVAEDLARRRLTELFGCEHANVQPHSGSQANMAVY
                     MTVLTPGDKVMGLDLSHGGHLTHGHPINFSGKLYEFHGYQVDRESETIDYDALLAQAQ
                     QVKPKMIVAGASAYPRTLDFKRIREACDACGAYMMVDIAHIAGLVAAGIHPSPIPYAD
                     FVTSTTHKTLRGPRGGVVMCKEKYAADLDRTVMPGIQGGPLMHIIAAKAVAFKEALQP
                     EFKAYQQRIVRNAAVLAEELKRLGYRLVAGGTDTHLMLVSFIGTKLTGKKVENTLDAA
                     GITVNKNTVPFDPEKPFVTSGIRIGTPAVTTRGMGPDEMKAIAGLIHRAIEGRKDEEA
                     LKGIAAEVARLCDRFPLYREL"
BASE COUNT         1222 a         1939 c         2263 g         1039 t
ORIGIN      
        1 cggggcgagc gccggcttcg gcatcatgct ctcggtccac aattccctct gctgcgagat
       61 catcaagctg ttcggatcgg agacgctcaa gcagaaatac ctccccgcca tggcggcggg
      121 ggagtgcatc ggcgcctact gtatcaccga gccggacgcc gggacggacg tggcctcgct
      181 gaagacgacg gcggcggacg gcggcgacgg ctgggtgctc aacggcacga agagctatgt
      241 gactaacgga tccttcgccg ggctcttcgt cgtgttcgcc aagaccgatc cggatgccgg
      301 gcacaagggg atttcgtgtt tcctcgtgcc ggcgggcacc ccgggcctga tgatcgggaa
      361 ggcggagcgg aagtgcggga tcaaagcttc ggacacgcgc gaggtcagtt tttccgacgt
      421 gcggataccg aaagaccaca tcctcggcgc gccggggcag gggttccgga tggcggtgac
      481 gattctgaat tcggggcggg tcggggtggc tttccagtcg atcgggatcg cccaggcggc
      541 tctcaatgag gcgctgaagt acgccaagga gcgcaagcag ttcaaccaac cgatcgccaa
      601 tttccaggcg atccagttca aactggccga catggcgctg aagatcgatg ccgggcggct
      661 cctcgcctac cgggccgcgc agctcaagga tgccggagag ccctgccacc gggaggcctc
      721 gatggcgaag ctgttctgct ccgaaatggc caatgaggtc gccaacgaag ccgtacagat
      781 acacgggggc tacggttaca tcaaggagta cgctgtcgag cgttatttcc gcgacgcgcg
      841 ggtgacgatg ctctacgagg gcacgtcgga ggcgcagcgg atggtcatct cacgcgactt
      901 gctgagggag tagcgccgtg ctggaaaaga ggcacctcga ataccgcgag cagatccgcg
      961 ccttcatgga ggacaagatc aaaccgctgg cgcacacgct ggatgtggaa cagcgcgtcg
     1021 ctcacgagca catgaagccg ctcaccgaca tggggctcat gtcgatgctg atcccggaga
     1081 agtacggcgg gcgtccgacc gacacggtga cgtacacgat cgcggtggag gaagcttcgc
     1141 gggtgtgcgg gtcgaccgga attctgctgg ccgcgcacaa ctccctcggc tgctacccga
     1201 ttctcgcgtt cggcaccgag gcccagcggc agcgctatct gccgcgggcg gcccagggcg
     1261 agattctcgc cttcggactc tcggagccgg acgcaggctc ggatgcgggc gggacgcgga
     1321 cgatggcggt gaagaaggcc gaccactggc tcatcaacgg gtcgaagtgc tggatcacct
     1381 cggcgacgcg cgcgttcgcc accatcgcct cggcccggac ctcggatgat cccgagaaca
     1441 aggagatcac ctgcttcatt ctcgagaagg actggccggg gtacgcggtc ggcaagaagg
     1501 agaacaagct ggggctccgg ggctcggaca cggcctttct ccatttcgac gacttgaaag
     1561 cgccgctcga cgcgcaactg gggatagtgg gggacgggtt caagcagatg ctcaagacgc
     1621 tcgacggggg gcggatctcg atcgcggcga tggcgctcgg gctggcccag ggcgctttcg
     1681 acgaggcgct ccggtacagc gcgagccgcg tccagttcgg caagccgatt tcgtacaacc
     1741 aggcgatcca gcacaaactc gccgacatgg cgacccagct tgaggcggcg cgcctgctcg
     1801 tctacgagtc ggcccgcatg aaagacgccg gggcgccgtt cggccactac tcggccatgg
     1861 cgaagctcta cgcctcggag atcggggtgc gggtgtgcgc ggacgcgatg ggcattctcg
     1921 gtgccatcgg ctactacacc gggccctacc cggtggagcg gatgtggcgc gacgtgaaac
     1981 tgtgcacgat cggcgaaggg acatcggaga ttcagaagct cgtcattgcc cgctacctgc
     2041 tgaagcaggt gcaggagaag agttagcaga agcggcccgc cgggcgcagt gtccggcggg
     2101 ccgggccgtc tgcagccgcg cccggcggtg gctttgcaga aaggccacgg ccgcggggga
     2161 ggcgcgggag cggacggtga gtgatggttc tcacaactgg tgaaggaggc ccggcgccta
     2221 tgaccccgac agatttcatc tggatgaacg gcaagctgat cccctggaag gatgcgacca
     2281 ttcatatttg ctcccacgtg attcactacg gttcgtcggt gtttgaagga atgcgggtgt
     2341 acaagacgcc gaaagggccg gcggctttcc gtctccggga gcacacccag cggctcctga
     2401 actcggcgaa gatctaccgg atgacggtgc cctatacggt cgaggagctg gaccgggcga
     2461 tactggagac cgtggcggcc aaccgcctcg aggcctgcta cgtccggccg atcgtctacc
     2521 gggggtacgg cacgctcggc gtggacccga ccaagtgccc gatcgatgtg gcgatcgcgg
     2581 tgtggccctg ggggcagtac ctcggcgagg aggcgctcgc caagggggtg tccgtgtgcg
     2641 tatcctcgtg gcaccgcttg gcgccgaaca cgatgccggc aatggccaaa gccggagcca
     2701 actacatgaa cagccagctc atcaagctcg aggcgctctc ccacgggtac gttgagggga
     2761 tcgccctcga cacctcgggg aatgtgtcgg agggctcagg ggagaacgtg ttcctcgtgc
     2821 gcaacggcgt gctcgtgacc ccgaccttcg cggcctcgat ccttccagga atcacacggc
     2881 gctcggtgat ccggatcgcc gaggatatgg ggatcaaggt gatcgaagag aacgtgccgc
     2941 gcgaggcgct ctatatcgcc gacgaagtct tcctcaccgg ttcagccgcg gaaatcacgc
     3001 ctatctcccg gatcgacaac atcgtcatcg gagagggaaa cgcgggaccg atcacgcgga
     3061 aaatccagaa ggcgtttttc gacattctcg agggccgagc cgaggaccgc tacgggtggc
     3121 tcacgctcgt gcccaagccg aagaacgcgg cggtgtagga ctatgaagtt cgcgatcggg
     3181 agcgaccacg ccgggttcga gatgaaagag cgggtcaagg agatcctgcg cgaactcggc
     3241 caggaggttg aggatgtcgg gacgcattcg aaagaatcgt gcgactaccc cgacttcgga
     3301 gtccgggtgg cgcgcatggt gcgcgagcac cgggcggagc ggggagtgct ggtgtgctgg
     3361 accggcaacg gcatgaccat cgccgccaac aaagtgcccg gggtgcgggc ggggctggcg
     3421 ctcaacgccg acatggctta tctcacccgc cgccacaacg acgccaacgt gctcaccctc
     3481 gcccagaagt acactcccga cagcgagctt cgggaaatcc tccgccggtt catcgagacc
     3541 gaattcgagg ggggacgaca cgagcggcgg ctgaacaaga tccgggcggt cgagccggga
     3601 aacgggacgc cctccccgcc acagaacgca tgatttcttt gtccttccga taattagctg
     3661 atccatagca agttatggtg gtcagttgaa acatcgcatt atgtttgtcc gggccgccgt
     3721 gctggcggtg ttggtgacgg tcgggccggc cggggacagc ggcgcacaag aacggttctc
     3781 ccgttttcgc ctggtgacgc aggacggggg cgtggtggag ggttttcggg gacggctcga
     3841 gggtcaggtc tttcgcggga cgacccgggc cggcgaccag tttgaaatcg cctgcgaccg
     3901 cctcatccaa ctggagcagt atcggggccg ccggacgggc gccgggttgg cgctggggat
     3961 tctctcaggg ctgtcagtcg gggttgtcta catgcttctg aatgacgata cgagcgacga
     4021 agcggggacg atcccgcgga cagtggcggt gttcggaggt ctggcggccg ccggcggggt
     4081 ggtcggggcg gctatcggcg gcgcgaccac gctctgggac ccggtgccgc tggggcgggg
     4141 ggagatcgga ggggcacggg aactcgaggg gacggccgcc gtggtctttc gcttctaagg
     4201 ccggttcact caggcgcggg ctcgcggctc cacaggaaat tgggacgaac ataggcgacc
     4261 tcgaagtcga accgctggac gttgcggaac gacggggctc ccctctctgc cgtctgctcg
     4321 gccgtttcca gcacgagggt gcgcaggcgc agggcggcgg cctcggcaat ccgccggtgg
     4381 agtagcgccg actgagcgcc cgaggcgcgg tagtccttgt cggtggcggc gaaatcaagc
     4441 catccgaaat cgccgtgcac aaacagagcg gccccggcga tcatccgcca gccgtcgaaa
     4501 gccccgtaaa tccgccagcg gggccgtccc accagcgtgg caatccactc gacaatcgcg
     4561 tcgccccact cgaaggcggt gcagaccagc tcgccgaaac ggagcgcctg gtcgcggccg
     4621 atctcgcgca ccgtgacgct gagttcggat gccggcggcg gcgaggtgtc gcggtagagt
     4681 ttcacccagt tgttgtgcca gcggagaccg cgagccccaa ggacctggcg ggcgttttcg
     4741 ggtgcgccga ccgggcaggt ctggatgaag aagcgcttga caccggcagc ccggtagtga
     4801 tcgaggcagc ggtcaacgtc aacgggatca atcgcgggtt gcaggccgaa gccgatcacg
     4861 cggttgaata cgagcacatc gaggccgggg acaagcacgc cggtcatggg gctgcattca
     4921 atcacgcggg cgccgagccg gcgggcaatc ttgtcggggg cggctgcgaa aacatcgacc
     4981 atggccgcga tttcgatcag ttccagcgac cgggccagct cacgcgacac ggggtggatc
     5041 atgtccttct cctgcaggca attctaccaa gggggtgacg cgcaccttac cgcttatacg
     5101 cctcaatggc caaggtcgtt gacccggagg tgtgtgtcaa gcgcttaagt cgttgacctc
     5161 gcgggtgatt ttgcatacat tgggcgggat ggtgaaagcg tttgcaaaac agggagatag
     5221 gcgatgtcat atttgaaaca gaccgacccc gagatctatg aagcgatgca gcgcgaaacc
     5281 gaccggcagg cgcacaagct ggaactgatc gcgtccgaga acttcgtctc tctggcggtg
     5341 atggaggccg cgggcggagt catgaccaac aagtacgccg agggatatcc gggcaagcgg
     5401 tactacggcg ggtgcgagtg ggtggacgtg gccgaagacc tggcccgtcg gcgcctcacc
     5461 gagttgttcg gatgcgagca cgccaacgtc cagccgcact ctggttccca ggcgaacatg
     5521 gcggtgtaca tgacggtgct gacgccgggg gacaaggtca tggggctgga cctgtcgcac
     5581 gggggtcacc tgacccacgg ccacccgatc aatttctccg gcaagctcta cgagttccac
     5641 ggctaccagg tggaccggga gagcgagacg atcgactacg atgcgctgct cgcccaggcg
     5701 cagcaggtga agccgaagat gatcgttgcc ggagcctcgg cctacccgcg gacgctcgat
     5761 ttcaagcgga ttcgggaagc ctgcgacgct tgcggggcct acatgatggt cgacatcgcg
     5821 cacatcgccg ggttggtcgc ggccgggatc catccctcgc cgatccccta tgcggatttc
     5881 gtgacctcga cgacgcacaa gacgctccgg gggccgcgcg gcggggtggt catgtgcaag
     5941 gaaaagtatg ccgccgatct cgaccggacg gtgatgccgg ggatccaggg gggaccgctc
     6001 atgcacatta tcgcggccaa agcggtggcc ttcaaagagg cgctccagcc ggagttcaag
     6061 gcctaccagc agcgcatcgt caggaacgcc gccgtcctgg ccgaggaact caagcgcctc
     6121 ggctaccggc tggtcgccgg cggcaccgac acccacctga tgctcgtgtc gttcatcggc
     6181 accaagctga cggggaagaa ggtcgagaac acgcttgatg cggcggggat cacggtgaac
     6241 aagaacaccg tgccctttga ccccgagaag ccgttcgtca cttcgggtat ccggatcggg
     6301 acgccggcgg tgacaacccg cgggatgggg ccggacgaga tgaaagcgat cgccgggctg
     6361 atccaccggg cgatcgaagg ccgcaaagac gaggaggcgc tcaagggcat cgcggccgag
     6421 gtggcgcgat tgtgcgaccg cttcccgctc taccgggagc tgg
//
LOCUS       DAOPOE010000139         6458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_13128_length_6458_cov_5.309230, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000139 DAOPOE010000000
VERSION     DAOPOE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13128_length_6458_cov_5.309230"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKM94_13045"
     CDS             complement(<1..569)
                     /locus_tag="PKM94_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67603.1"
                     /translation="MNGIRLILAVATGALLAACAHAAGGDRIYARVVTVDGDEYEGLI
                     RWDKNEASWADMLDGIRSSEKGGDDSDRTTRGKTERRIKIFGFTVGTESDSWWSPSAQ
                     CGVRFGHLRRLQPLGDDRALLEFKSGQRVELSGGSTDLGDGVREIVVEDPGEGEVHLV
                     WGDIEEVIFQLTDKDSESVFGERLYGTLTT"
     gene            complement(664..3570)
                     /locus_tag="PKM94_13050"
     CDS             complement(664..3570)
                     /locus_tag="PKM94_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD67604.1"
                     /translation="MLRCLDTIRPANDAAIRARHPWRALFIRTAFSAALLCARPAAAG
                     ELSDLRVNDDAGSAIQADPRVAVAANGTFAVVWSDRRLGANGDIFIQCFDSAGARLGG
                     NRRVSDDPVGSAKAQPAVAADYAGRFVVAWLDYRGGFPIDPQVYLQRFSGAGTPLGGN
                     VRITDTIAIRQAPDLAFSSWGGVLVWGEYSGGHWGILGKRIDTAGNVTGPLWRANTVL
                     DSARHAPRVACSPRGWFVVAWQDKRFGHHDIFVQRFDAAGQRVGPNVKVNADAGSAEQ
                     TAPDVAADGAGRFTVVWTDYRNGTGNPDIYAARFDTTMTPLTANLRLNSVGTNPVQRG
                     AAIGADRMGNTVIVWGDSTTSGWDVIGQMVDADGVVREANFAGSTATAGNQYNPDAAA
                     DGAYRYVVWTDQREDVAWDIFASVKRYNSPGLTVTPLDLAFDMRVGGPIPPAQTIALD
                     HRGYNALDFEVVSHSRWLTVAPLGGTTPDSVAVAIASDTLPNGVYLGYVRFRAPMASD
                     SAIDAAVRLEVRSARLSVSVDTIRALAPEGYDTVLHEAFTIANDGPGAMTWTAAESAP
                     WLGLGSTGGSAPSVVVAAIDARALAAGTYAAEIVIDAGAAENSPATVAVVLEVRDDLP
                     VLAVAPPAIATTVADPADFDTAFTVANAGGGSLIWSVSGGAAWLEAEPAAGVGPGTVR
                     LTANAGQLAPGANTAVLTVNAPGSYTDSASVGVSLTWAPLRTDTIVVEPATVRPGQSG
                     VTSIRARIHNRLAALVLPLQCSPGLISFDSLQFGEGLPYFLDRYYRIEPAASAIVVQL
                     VRTTSDTALAPGEYLLARIYFTADSVTGIVSLRALDSDSLSPVVVTDEGDTKFPVVQA
                     GRVALESPGGVGEEGEREAAGAVGLSQNFPNPFNSGTTLSFTVPKTSPVRLEVFNVLG
                     RRVAVLAEGVHTPGVYRVHWAGKDEQGGEAPSGVYFYRLETADTALVAKMILVK"
     gene            complement(3779..4063)
                     /locus_tag="PKM94_13055"
     CDS             complement(3779..4063)
                     /locus_tag="PKM94_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD67605.1"
                     /translation="MTKADLVERVAEKTGLTRTDVAVVVDSFLDTVKKSLETGHNIEI
                     RGFGTFKVKLRKARKARNPRTGDVVPVPDRKVPVFKPSNEFKNMITKLEI"
     gene            complement(4255..5157)
                     /gene="sppA"
                     /locus_tag="PKM94_13060"
     CDS             complement(4255..5157)
                     /gene="sppA"
                     /locus_tag="PKM94_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOD67606.1"
                     /translation="MARTRDVVVGVVIAVVFLGAIGFFGLMIAGMLAESGEFRFAGLG
                     GNIGLVEVYGTIDEEMGRPIIDLLDEWKDNSGIRAIVLHVNSPGGGVAISQEIYDAVK
                     RARDVKPVVASMASVCASGGYYIACAADRVVANPGSLTGSIGVIFQFYTAGKLMEKVG
                     IETETVKSGELKDVGSLGRAMTEEERLMLRSVVMDTYEQFVQAVADGRGLDPEQIYPL
                     ADGSIFTGLQALNLHLVDTLGGLNEAIQVAADLADLEGTPRVVRPVKRRQISVFDLLG
                     GLLRATQTDLVREMSGPELLYLYR"
     gene            complement(5170..>6458)
                     /locus_tag="PKM94_13065"
     CDS             complement(5170..>6458)
                     /locus_tag="PKM94_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOD67607.1"
                     /translation="GLWQFIASTGRLYGLDRDWWIDERRDPIKSTHAACRFLKDLYQK
                     FGSWELAMAAYNGGPGRVERTIAKQGTRDFWKLNLRRQTMDYVPLIMAAAMISKEPEK
                     YGFESIEFEDELTWDEVSIDRCLDLTVVADAVGCTVGELEDLNPELLRKFTPPNVKDY
                     RLKIPSGSRTKFLAAYESMESPKESSWVRHTIRRGETVSSIASRYGVSQYSILAANNM
                     SSRSKIYAGRTLIVPVPLDGAPSGGGDGRSYESRNGVYVVRSGDTMWDIAKAFGTSVD
                     ALRRSNAIGRGARIYVGQKLRIPGGSHPADAGRTYAGTPAASKKSSGSSQRYTVRSGD
                     TLWEIARKFGTTTSNIRRLNNLGRSSRIYPGQVLTVGGGSGAAADYVIHRVRRGESLS
                     QIAQKYRTTIAKIIETNALSNPDQLQVGDQLRIYTE"
BASE COUNT         1082 a         2247 c         1988 g         1141 t
ORIGIN      
        1 gtggtcaggg tcccatacag ccgttctcca aagaccgact cgctgtcttt gtcggtgagc
       61 tggaaaatga cttcctcgat atccccccag acgaggtgca cttcgccttc gccgggatcc
      121 tcgaccacaa tctcccgcac gccgtctccc agatcagtcg aacctccgga cagttcgact
      181 ctctggcccg acttgaattc caaaagcgcc cgatcgtcgc ccaggggctg caaccgccgg
      241 aggtggccga agcgcacgcc gcactgggcg gagggggacc accaggagtc cgattcggtg
      301 ccgacggtga atccgaaaat cttgatccgc cgctcggtct tgccccgcgt cgtgcggtcg
      361 ctgtcgtcgc cgcccttttc ggagcttcgg atgccgtcga gcatatccgc ccaggaggcc
      421 tcgtttttgt cccagcgaat caatccctcg tactcgtcgc catcgaccgt taccaccctc
      481 gcgtagatgc ggtcgccgcc tgcggcgtgc gcacacgccg ccaggagcgc tccggtcgcc
      541 acggcgagga tcaagcgaat gccgttcatg tctgtcctcc gacacctttc cggtgaccgc
      601 tgtctttatc tgcgtttacg agattgccgg gaaaaggttt agcccgaggc cgaatttgaa
      661 cccctatttg accaggatca tcttggccac cagcgctgtg tccgccgtct ccaggcggta
      721 gaaatagaca cccgaggggg cctctccccc ctgctcgtcc tttcccgccc agtgaacgcg
      781 gtagacgccg ggggtgtgca ccccctcggc cagaaccgcc acccgtcgcc cgagcacatt
      841 gaacacctcg agccggaccg gcgaggtctt cggcaccgta aacgacagtg tcgtgccgga
      901 gttgaacgga ttggggaagt tctgcgacag cccgaccgcg cccgccgcct cccgctcccc
      961 ctcctcgccg accccccccg ggctctccag cgccacccgc cccgcctgca ccacgggaaa
     1021 ctttgtgtcc ccctcgtcgg tcaccaccac cggcgagagc gagtcgctgt ccagcgcccg
     1081 cagcgagacg attccggtca cgctatcggc ggtgaagtag atgcgggcca gaaggtactc
     1141 gcccggcgcc agggccgtgt cgctggtcgt acggaccagt tgcacgacga tcgccgaagc
     1201 ggccggttca atccggtaat agcggtcgag aaagtagggc agtccctccc cgaattgcag
     1261 ggaatcgaac gaaatcagtc cgggcgaaca ctgcagcggc agaacgagcg ccgccagccg
     1321 gttgtggatg cgtgcccgga tcgaggtcac acccgactgg cccggcctga cggtcgccgg
     1381 ctcgactaca attgtgtcgg tgcgcagcgg cgcccacgtc aacgagacgc caacggacgc
     1441 cgagtcggta tacgagcccg gcgcattcac ggtcagcacc gccgtgttgg ctcccggtgc
     1501 gagctgcccc gcgttggccg tcagccggac cgtccccggc cccacgccgg cggcgggctc
     1561 cgcctcgagc caggctgcgc cgccggagac gctccagatc aggcttcccc cgccagcgtt
     1621 ggcgacggtg aaagcggtat cgaaatcggc cgggtcggcc acggtcgtcg caatcgccgg
     1681 cggggccacg gccagcaccg gcaggtcatc gcggacctcc agcaccacgg ccaccgtagc
     1741 gggagaattc tcggcggcac cggcatctat gacgatttcc gcagcgtagg ttccggcggc
     1801 cagggcgcgg gcgtcgatag ccgcgaccac gaccgagggg gccgacccgc cggttgatcc
     1861 gaggcccagc cagggcgcgc tctccgccgc cgtccacgtc atcgcccccg ggccgtcatt
     1921 ggcgatcgtg aaggcctcgt ggagcacggt gtcgtatccc tccggggcca gtgcccggat
     1981 ggtgtcgacg gagacgctca accgggccga gcggacctcg agccgcacgg ccgcgtcgat
     2041 cgccgagtcg ctcgccatcg gcgcgcgaaa gcgcacgtac ccgaggtaga cgccgttggg
     2101 aagggtgtcg gaggcgatcg ccacggcgac cgaatccggc gtcgttccgc cgagcggcgc
     2161 cacggtcagc caccggctgt gcgacaccac ctcgaaatcg agagcgttgt acccccggtg
     2221 gtccagggcg atcgtctggg ccggcggtat cggtccgccc acgcgcatgt cgaatgccag
     2281 gtcgagcggc gtgacggtca gtcccgggct gttgtaccgc ttgaccgagg cgaaaatgtc
     2341 ccaagcgacg tcctcgcgct ggtccgtcca caccacatag cggtaggcgc cgtcggccgc
     2401 cgcatcggga ttgtactgat tccccgccgt ggctgtgctt ccggcgaaat tcgcttcccg
     2461 caccacgccg tcggcatcga ccatctgccc gatcacgtcc caccccgagg tggtcgaatc
     2521 cccccacacg atcaccgtgt tgcccatccg gtcggcaccg atcgcggcgc cgcgctgcac
     2581 cgggttcgtc ccgacggagt tgagccggag gttggcggtg agcggcgtca tggtggtgtc
     2641 gaagcgggcc gcgtagatat ccggattgcc cgttccgttg cggtagtcgg tccagacgac
     2701 cgtgaaccgg cccgcgccgt cggccgctac gtcgggggcc gtctgctccg cggacccggc
     2761 gtcggcgttg actttcacgt ttggccccac gcgctggccg gccgcgtcga atcgctgcac
     2821 gaagatgtcg tggtgtccga accgcttatc ctgccaggcc accacgaacc atccccgcgg
     2881 cgagcaggcg acccgcggtg cgtggcgcgc gctgtcgagc acggtgttcg cccgccacag
     2941 cggcccggtg acattcccgg ccgtatcgat gcgcttgccg agaattcccc agtgcccgcc
     3001 ggagtactcg ccccagacga gcaccccgcc ccatgaggag aacgccagat cgggcgcctg
     3061 gcggatagcg atcgtgtcgg tgatccgcac gttcccgccg agcggcgtcc cggccccgct
     3121 gaaccgctgc aggtacacct gggggtcgat gggaaatccg ccgcggtagt ccagccaggc
     3181 gacgacaaag cgcccggcgt aatcggccgc cactgccggc tgcgctttgg ccgacccgac
     3241 cgggtcgtcg ctcacgcggc ggttgccgcc cagccgcgcc ccggcgctgt cgaaacactg
     3301 gatgaagatg tcgccgttgg ccccgaggcg gcggtccgac cagaccaccg caaaggtgcc
     3361 gttggctgcg acggccaccc gggggtcggc ctgaatggcc gacccggcgt cgtcgttgac
     3421 gcgcaggtcg ctcagctcgc cggccgcagc cggccgggcg cacaggagag ccgcgctgaa
     3481 cgccgtgcga atgaaaagag cccgccacgg gtggcgggct cgaatcgccg catcgttggc
     3541 tggacggatc gtgtcgaggc agcgcaacat ccgtgttgtc cgcagcaacg aagtaacttg
     3601 gaggccttcg ggccggcagg caatcctgcc gccccgccca gcaatcaaca caacctatct
     3661 gattttcttc ttatggcgat tggcacgtct gcgctttttg cgcttgtgcg tcttgatttt
     3721 gagccgtttc cgcttcttgc cgctgggcat tgatcctcca aaatcactgg tgcgtggttc
     3781 agatctccaa cttggtgatc atgtttttaa actcattgga cggcttgaac accgggacct
     3841 tgcggtccgg caccggcaca acgtcgccgg tccgtgggtt ccgggccttc cgagccttcc
     3901 gtaatttgac cttaaacgtg ccgaaaccgc gaatctcgat gttgtgtccc gtctcgagcg
     3961 atttcttgac ggtgtcgaga aacgaatcaa cgacaacggc gacgtccgtt ctggtcagac
     4021 cggtcttctc agcaacccgc tcaaccaaat cagctttcgt cacagcgttc tccctcgacc
     4081 aggtttatgc attcgtgtta ttctctgttt cggtccagtc cttctccgtc cccggggccg
     4141 ataagttaac cctttgagac acgaaaagat acacaccgta gaggaaacac gttgccggac
     4201 tggttgtcaa ctatttttcg cgcccaaacc caaaaaaaac gccgtaactg actgttagcg
     4261 gtacaaatac agcaattccg ggccgctcat ctcccggacc aggtccgtct gcgtcgcccg
     4321 gagcaacccc ccgagcaggt caaagactga aatttggcgt cttttgaccg gccggaccac
     4381 cctcggcgtc ccctctaagt cggccagatc cgccgccacc tgaatcgcct cgttcaaacc
     4441 gcccaaagta tccaccaggt gcaggttgag ggcctggagt cccgtgaaaa tcgacccgtc
     4501 ggcgagcgga tagatctgct ccgggtcgag tccccgcccg tccgccaccg cctgcacgaa
     4561 ctgctcgtaa gtgtccatca caaccgaccg gagcatgagc cgctcttcct cggtcatcgc
     4621 ccggccgagc gagccgacgt ccttgagctc gcccgacttg accgtctccg tctcgatccc
     4681 caccttttcc atcagcttgc cggcggtata gaactggaaa atgacgccga tcgagccggt
     4741 gagcgacccg ggattggcga ccacgcggtc ggcggcacag gcgatgtagt agccgccgga
     4801 ggcgcacacg gaggccatcg aggcgacgac cggcttgaca tcacgggccc gtttcacggc
     4861 atcgtagatt tcctgggaga tggccacgcc gccgccgggg gagttgacgt gcagcacgat
     4921 ggcccggatg cccgagttgt ccttccattc gtcgagcagg tcgataatcg ggcgccccat
     4981 ctcctcatcg atcgtgccgt acacttcgac gagtccgatg ttgccgccca gcccggcgaa
     5041 ccggaattcc cccgactcgg ccagcatgcc cgcgatcatg aggccgaaga acccgatcgc
     5101 ccccaggaag acgaccgcaa taaccacccc gaccactacg tcgcgcgtcc gcgccatacc
     5161 cgacgtcctt tactccgtgt agatcctcag ctggtccccg acctgaagct ggtcggggtt
     5221 actcagcgca ttcgtttcga taatcttcgc gatcgtggtc cggtacttct gggcaatctg
     5281 gctgagcgat tcgccgcgcc ggacgcggtg aatcacgtag tcggcggcgg cgccggaacc
     5341 accgcccacg gtgaggacct gcccggggta aatgcggctg gagcgcccga ggttgttcag
     5401 gcggcggatg ttcgaggtcg ttgtcccaaa cttccgcgcg atctcccaca gcgtatcgcc
     5461 ggagcggacc gtgtatctct gcgaactgcc ggaggacttc ttcgaagccg ccggcgttcc
     5521 ggcgtaggtc cgacccgcgt cggccggatg ggatccgccg gggattctga gtttctggcc
     5581 cacatagatg cgcgccccgc ggccgatggc gttgctccgc cgcagcgcat ccacggacgt
     5641 cccgaaggct ttcgcaatat cccacatggt gtcgcccgag cgcaccacgt agacgccgtt
     5701 gcgcgattcg taggaccttc cgtcgccccc gcccgacggc gcgccgtcaa gcggcacggg
     5761 cacgatcagc gtcctgcccg cgtagatctt cgagcggctg ctcatgttgt tcgcggccag
     5821 gatggagtac tgcgacaccc cgtagcggga ggcgatggag ctgaccgtct ccccccggcg
     5881 aatggtgtgc cgcacccagc tcgactcctt gggcgattcc atgctctcgt aggcggccag
     5941 gaatttcgtc cgcgacccgg aagggatctt cagccggtag tccttcacgt tcgggggcgt
     6001 aaacttgcgc agcagttccg gattgaggtc ttccagctcg cccaccgtgc agccgactgc
     6061 gtccgccacc accgtgagat cgaggcaccg gtcgatcgaa acttcgtccc aggtgagctc
     6121 gtcttcgaac tcgatactct caaaaccgta cttctcgggt tctttggaga tcatcgccgc
     6181 cgccatgatg agcgggacgt agtccatcgt ctggcgccgc aggttgagtt tccagaaatc
     6241 ccgcgtcccc tgcttggcga tcgtccgctc cacccggccg ggaccgccgt tgtaggccgc
     6301 catggccagt tcccacgacc cgaatttctg gtagagatcc ttcaggaaac ggcaggcggc
     6361 gtgggtcgac ttgatcgggt cgcgccgctc gtcgatccac cagtcgcggt ccaacccgta
     6421 gaggcgcccg gtggaggcga tgaactgcca cagtcccg
//
LOCUS       DAOPOE010000140         6156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_13988_length_6156_cov_6.090969, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000140 DAOPOE010000000
VERSION     DAOPOE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13988_length_6156_cov_6.090969"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKM94_13070"
     CDS             <1..180
                     /locus_tag="PKM94_13070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOD67608.1"
                     /translation="RGTTTAGVWHYAAKDLTVDNIKLLGQYLMTEYALPFEIVSVLLV
                     AAMIGAIVLARKERS"
     gene            180..479
                     /gene="nuoK"
                     /locus_tag="PKM94_13075"
     CDS             180..479
                     /gene="nuoK"
                     /locus_tag="PKM94_13075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOD67609.1"
                     /translation="MLPYLVLAMILFATGLFGVLTRRNTVGILMSLELMFNAANINFV
                     TFNRFVETTPLVGQLFAMFVIVIAAAEAVVGLAIVLLIYRNWRGIDSDHYSVMKW"
     gene            488..2614
                     /gene="nuoL"
                     /locus_tag="PKM94_13080"
     CDS             488..2614
                     /gene="nuoL"
                     /locus_tag="PKM94_13080"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOD67610.1"
                     /translation="MTEYSFLICVLPLLSFLLIVFFFRWNEKLSSLFSIAMILASFLL
                     SVIVLIETIGRHGAAYEASYRFLDFPNFHLQIGILVDALTAIMLVVVTTVGACVQIYS
                     LGYMHNDPRFSRFFAYLSLFLFSMLGLVLANNFFMIFIFWELVGLTSYLLIGFWFEKK
                     SAADACKKAFLTTRTGDLGFIVGLLTIGIYFGTFNYGEVFALAGSGTIPAGILTLAAV
                     GVFCGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVARSMALFAASAE
                     AALVVAIIGLTTSFIAATIALTQFDIKRILAYSTVSQLGYMIMALGLFGYDTAHARHS
                     VGYYAGTFHLMTHAFFKGLLFLGAGSVIHAVHTNDIREMGGLRTKMPRTSYTFMIASL
                     SIAGIFPLAGFWSKDEIVAATLDHPVFFVLTLAIAFMTAFYMWRLCFLTFFGEPRDPH
                     RFAHAHESPANMTYPLVFLAVLAVSAGWVGLPMEWLTGHGIASFLYFGAEAPEHHTPA
                     YWLMGVSLLVGLAGIGLAAAMYYWKKISADSVVRAVRPVHAFLYHKWYFDELYFAAIV
                     NPLSRFAGLLWKFDAGIVDGAVNGTAWLTILWADIKQWFDTWIVDGAVNGAGWVVSGF
                     GGLLRYLQSGRPQFYALTIGAVLVFAALAKLEANVAGARFPYLTVIFGAGVILLGLFA
                     RSGRDAGGPDEPSEAKEFQVTVEEKR"
     gene            2616..4094
                     /locus_tag="PKM94_13085"
     CDS             2616..4094
                     /locus_tag="PKM94_13085"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOD67611.1"
                     /translation="MLSLLVFVPLAGMLIVMLLPKDNKAAIRWTAFGVSLVPMVLSFW
                     VTWDYFVNKAASHMLAYVEGPFDWIPSLNIQYYLGVDGISVPMLALTGLLSSISLLAS
                     FNIEKRVKEYFAFFLLLEAGMMGVFVALDFFLFYVFWEVMLVPMYFLIGIWGGPRKEY
                     AAIKFFLYTLFGSIFMLVAILILYFSSTPHTLNMVTLLETGPGLGHGLQMVCFAFFFI
                     AFAIKVPVWPFHTWLPDAHVEAPTAVSVILAGVLLKMGTYAMLRISWPMFPSALATFG
                     IWIAVLGAIGIIYGALVSMAQKDLKKLVAYSSVSHMGYCMLALGAYTSVTALAGVMFQ
                     MISHGLITGALFLLVGVLYDRAHTREIAAFGGLGVKVPVYTGLMVLFGMASLGLPGMS
                     GFVSEFMVFVGAFMGTNKILVGISVLGVVLGATYILRMVQRVFLGEFDLARWGGLTDI
                     NLRELICVAPLAVLTIAIGVYPKPVEVLMGATLQNLVNLMVR"
     gene            4099..5604
                     /locus_tag="PKM94_13090"
     CDS             4099..5604
                     /locus_tag="PKM94_13090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOD67612.1"
                     /translation="MDVQYPDINLRYMLPEMFLFLWACVVIAYDAASRRRSETGAGYL
                     ALFGLVVTGLLLAFTGDGAGWGRMFVQDTAARFFKMVFLGAAFMAIGSSFGIMRQRIT
                     HHRGEFFGLILLSTVGMMFLASANELLTIYVGLELTTIPLYVLAAFYKDNKLSVEAGV
                     KYFIVGAFSSALLLFGISYLYGMAGTTDLLLLKISLSTTHQAVGTLGFILVLAIVCMA
                     AGIGFKLALPPFHQWAPDVYEGAPTPVAAFLSVGSKGAGLVAFAKVFAKGMEAFHPTY
                     MAPNDWGKLAAMLAAAAMIVGNTVAIRQTNIKRLLAYSSIAQAGYIMVGLVAMNRFGM
                     GSMAFYIFAYLFANMGAFACVAIFEDRTGSVQIDSYNGLARTSPLMAATFAVFLLSLA
                     GIPPLAGFFAKYYVFAAAIQSAQADASAGWLYWLVGIGLLTSVFALYYYARIIKAMYF
                     AKEESPYRMEYTPPALAVLAIGLAGLFVFGLYPEPVLRLAADITHSFLVIP"
     gene            5785..>6156
                     /locus_tag="PKM94_13095"
     CDS             5785..>6156
                     /locus_tag="PKM94_13095"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOD67613.1"
                     /translation="MLKRNFLSGVLVVVPLILTYLVLRFLLETLDGILRPPLAKLIGR
                     DIPLVGFVALIVLIFVAGVLVRSYVGHRLYGLGERWLVRLPLIRPIYSAAKQLLEALA
                     GPKMNAFKEVALIEYPRLGLWS"
BASE COUNT          881 a         1936 c         2012 g         1327 t
ORIGIN      
        1 cgggggacga cgacggcggg ggtgtggcac tacgcggcca aggatctgac ggtcgacaac
       61 atcaagctgc tcgggcagta cctcatgacc gagtacgcgc tcccgttcga aatcgtctcg
      121 gtgctgctgg tggcggccat gatcggggcc atcgtgctgg cgcggaagga gcggtcgtaa
      181 tgctgcccta cctcgtgctg gcgatgatcc tgttcgcgac cgggctgttc ggcgtgctca
      241 cccggcgcaa caccgtgggg atcctgatgt cgctcgagct catgttcaac gcggccaaca
      301 tcaatttcgt gacctttaac aggttcgtgg agaccacccc cttggtcggg cagttgttcg
      361 ccatgttcgt gatcgtcatt gcggcggcgg aggcggtggt cggtctcgcg atcgtcctgc
      421 tgatctaccg caactggcgc ggtatcgaca gcgaccacta cagcgtcatg aagtggtagg
      481 gaaggctatg acggagtatt catttctcat ctgcgtgctg ccgctcctct cgttcctcct
      541 tatcgtgttc ttcttccgct ggaacgagaa gctctcgtcg ctgttttcga tcgccatgat
      601 tctcgcctcg ttcctgctgt cggtgatcgt gctgatcgag acgatcgggc ggcacggcgc
      661 ggcctacgag gcctcctacc gcttcctcga tttcccgaat ttccatctcc agatcgggat
      721 cctggtcgac gccctcacgg cgatcatgct cgtggtcgtg accaccgtgg gagcctgcgt
      781 gcaaatctac tcgctcgggt acatgcacaa cgatccgcgg ttcagccggt ttttcgccta
      841 cctgtcgctg ttcttgttct cgatgctcgg gttggtgctc gcgaacaact tcttcatgat
      901 cttcatcttc tgggagctcg tcgggctcac ctcgtacctg ctgatcgggt tctggttcga
      961 gaagaagtcg gcggcggacg cctgcaagaa ggccttcctg acgacgcgga cgggggatct
     1021 cgggttcatt gtcgggctgc tcacgatcgg gatctacttc gggacgttca actacgggga
     1081 ggtgttcgcg ctggccgggt cggggacgat cccggcgggg attctcacgc tcgcggcggt
     1141 gggggtgttc tgcggggcgg tcggaaaatc ggcccagttc ccgctccacg tgtggctgcc
     1201 cgacgcgatg gagggtccga cgccggtctc ggcgctcatc catgcggcga ccatggtggc
     1261 ggccggtgtc tacctcgtgg cgcggtcgat ggcgctcttc gcggcctcgg ccgaggccgc
     1321 gctggtcgtg gcgatcatcg ggctgaccac ttcgttcatc gccgcgacga tcgctctcac
     1381 ccagttcgat atcaaacgga tcctggctta ttcgacggtg agccagctcg gatacatgat
     1441 catggcgctc gggctgttcg gctacgacac ggcccacgcc cgccactcgg tggggtacta
     1501 cgccgggacg ttccacctca tgacccacgc cttcttcaag gggctgctct tcctcggggc
     1561 cggttcggtg atccacgccg ttcacaccaa cgacatccgg gagatgggcg ggctgcggac
     1621 gaagatgccc cggacgtcgt acacgttcat gatcgcctcg ctctcgatcg cggggatatt
     1681 cccgctcgcg gggttctggt cgaaggatga gatcgtggcc gcgacgctgg accacccggt
     1741 gtttttcgtc ctcaccctgg ccattgcgtt catgacggcg ttctacatgt ggcggctgtg
     1801 cttcctcacg tttttcggcg agccgcgcga cccccaccgc ttcgcccacg cgcacgagtc
     1861 gccggcgaac atgacctacc cgctggtgtt cctcgcggtc ctcgcggtct cggccggctg
     1921 ggtcgggctg ccgatggagt ggctgaccgg gcatggcatc gcctcgttcc tctatttcgg
     1981 agcggaggcg ccggagcacc acacgccggc ctactggctc atgggggtgt cgctcctggt
     2041 ggggctggcc gggatcgggc tggcggccgc catgtactac tggaagaaga tttccgcgga
     2101 cagcgttgtc cgcgcggtcc ggccggtgca cgcgttcctg taccacaagt ggtatttcga
     2161 cgagctctat ttcgcggcga tcgtcaaccc gctcagccgc ttcgccggcc tcctgtggaa
     2221 gttcgatgcg gggatagtcg acggggcggt caacgggaca gcctggctga cgattctgtg
     2281 ggcggacatc aagcagtggt tcgacacgtg gattgtcgac ggggcggtca acggggcagg
     2341 ctgggtggtc agcggcttcg gcgggctcct gcgctacctc cagagcggcc ggccgcagtt
     2401 ctacgcgctg accatcgggg cggtgctggt gttcgcggcc ctcgccaagc tggaggccaa
     2461 cgtcgccggc gcgcgcttcc cctacctgac cgtaattttc ggggccggcg tgatcctgct
     2521 ggggttgttc gcgcgctccg gccgcgatgc cggaggaccg gacgaaccgt ccgaggccaa
     2581 agaatttcaa gtgactgtgg aggagaaacg atagcatgct gagtctcctg gtatttgtcc
     2641 ccctggcggg aatgctcatc gtgatgctgc tgccgaaaga caacaaggcg gccatccggt
     2701 ggacggcgtt cggggtgagc ctcgtgccga tggtcctgtc gttctgggtc acgtgggact
     2761 atttcgtgaa caaggccgcg tcgcacatgc tcgcgtatgt cgaggggccg ttcgactgga
     2821 ttccctcgct gaacatccag tactatctcg gcgtggacgg gatctcggtg cccatgctcg
     2881 ccctcaccgg gctgctctcg agcatttcgc tgctcgcgtc gttcaacatc gagaagcggg
     2941 tcaaagagta cttcgcgttc ttcctcctcc tcgaggccgg gatgatgggc gtgttcgtgg
     3001 ccctcgattt cttcctcttc tacgtcttct gggaagtcat gctcgtgccg atgtacttcc
     3061 tcatcggcat ctggggcggg ccgcgcaagg agtacgccgc aatcaagttc ttcctctaca
     3121 ccctgttcgg gtcgatcttc atgctggtcg ccatcctcat cctctacttc tcctccaccc
     3181 cccacacgct caacatggtg accctcctgg agaccgggcc ggggctgggg cacgggcttc
     3241 agatggtctg tttcgccttc ttcttcattg ccttcgcgat caaagtgccg gtgtggccgt
     3301 tccacacctg gctgccggat gcccacgtcg aggcgcccac ggcggtgtcg gtgatcctgg
     3361 cgggcgtgct gctgaaaatg gggacgtatg cgatgctgcg gatttcgtgg ccgatgttcc
     3421 cctcggccct ggcgaccttc gggatctgga tcgcggtgct cggggcgatc ggcatcatct
     3481 acggggcgct tgtgtcgatg gcgcagaagg atctcaagaa gctggtggcc tactcctcgg
     3541 tgtcccacat ggggtactgc atgctcgcgc tcggggccta cacgtcggtg acggcgctcg
     3601 cgggggtgat gttccagatg atttcccacg ggctgatcac cggggcgctc ttcctcctgg
     3661 tgggcgtgct ctacgaccgg gcgcacacgc gcgagatcgc cgccttcggc gggctgggcg
     3721 tcaaggtgcc ggtgtacacc ggcctcatgg tgctgttcgg gatggcctcg ctcgggctgc
     3781 ccggcatgtc gggcttcgtg agcgaattca tggtcttcgt cggcgccttc atggggacca
     3841 acaagatcct cgtcggcatc tccgtcctcg gcgtggtgct cggcgcgacc tacatcctcc
     3901 gtatggtgca gcgggtgttc ctcggcgaat tcgacctggc ccgctggggc gggctgaccg
     3961 acatcaacct ccgcgaactg atctgcgtag cgcccctggc cgtgctgact atcgccatcg
     4021 gcgtgtaccc caagccggtg gaggtgctca tgggggcgac gctgcaaaac ctcgtcaacc
     4081 tgatggtgcg gtagccgtat ggacgtgcag tatcccgaca tcaaccttcg ctacatgctc
     4141 cccgagatgt tcctgttcct gtgggcgtgc gtggtgatcg cctacgacgc cgcgagccgg
     4201 cggcggagcg agacgggggc cgggtacctc gcgctcttcg ggctggtggt gacgggcctg
     4261 ctcctggcct tcaccggcga cggggcgggg tgggggcgga tgtttgtcca ggacacggcc
     4321 gcccgcttct tcaaaatggt cttcctcggc gcggcgttca tggccattgg cagctccttc
     4381 gggatcatgc ggcagcggat cacgcaccac cggggggagt tcttcgggct gatcctcctg
     4441 tcgacggtgg ggatgatgtt cctcgcctcg gccaacgaac tgctcacgat ctatgtcggg
     4501 ctggagctga cgacgatccc gctgtacgtg ctggcggcat tctacaaaga caacaagctc
     4561 tcggtcgaag ccggggtcaa gtatttcatc gtcggggcgt tctcctcggc tctgctgctg
     4621 ttcgggatct cctacctcta cggcatggcg ggcacgaccg acctgctgct tttgaagatt
     4681 tccctgtcga cgacccacca ggcggtgggg acgctcggct tcatcctcgt gctggcgatt
     4741 gtctgcatgg cggccggtat cgggttcaag ctcgcactgc cgccgttcca ccagtgggcg
     4801 cccgatgtgt acgagggagc gccgacgccg gtggcggcgt ttctgtcggt gggttcaaag
     4861 ggcgcggggc tggtggcctt cgcgaaggta ttcgccaagg gcatggaggc ctttcacccg
     4921 acctacatgg ccccgaacga ctggggcaaa ctcgcggcca tgctcgcggc ggcggccatg
     4981 atcgtgggca acaccgtggc catccgccag accaacatca aacgcctgct cgcctattcc
     5041 tcgatcgccc aggccggcta catcatggtc gggctggtgg ctatgaaccg gttcgggatg
     5101 gggtcgatgg cgttctacat tttcgcctac ctcttcgcga acatgggcgc gttcgcctgt
     5161 gtggcgattt tcgaggaccg caccggctcg gtccagatcg acagctacaa cggactcgcc
     5221 cggacctcgc ccctgatggc ggccacgttc gcggtgtttc tgctctcgct ggccggaatt
     5281 cccccgctcg ccggattctt cgccaagtac tatgtcttcg ccgcggccat ccagtcggct
     5341 caggcggatg cctccgccgg gtggctctac tggctggttg ggatcggcct gctgacctcg
     5401 gtgttcgcgc tgtactacta cgcgagaatc atcaaagcaa tgtatttcgc caaggaggag
     5461 tcgccctatc ggatggagta cactcccccg gcgctggcgg tgctggcgat cggcctggcg
     5521 gggctgttcg tcttcgggct gtatcccgag ccggtgctgc ggctcgcggc cgacatcacc
     5581 cattcgtttc tggtgattcc gtagggcggg agaagcgggg gaaggatcaa gcgcccggcg
     5641 catcgcacga tgcaccgggc ggggccatgt cggggcagcg gtccccgctc cggttctgct
     5701 tgacagaccc cccggcaagt gatattctcc gaacagattg cgccgaatgg tgaggcaagg
     5761 acgcggatgg ccggaatcgg gtcgatgctc aaacggaatt tcctgtccgg ggtgctcgtc
     5821 gtcgtacccc tgatcctgac ctacctcgtc ctccgcttcc tgctggagac gctcgacggc
     5881 atcctccggc cccccctggc caagctgatc ggccgcgaca ttccgctggt cggcttcgtc
     5941 gcgctcatcg tgctgatctt cgtcgccggg gtcctggtcc gcagttatgt cggccaccgg
     6001 ctctacgggc tgggcgaacg atggctcgtg cggctgccgc tgatacggcc gatctactcg
     6061 gcggccaagc agctcctgga ggcgctggcc gggcccaaga tgaacgcctt caaagaggtg
     6121 gcgctcatcg agtacccaag gctcggcctc tggagc
//
LOCUS       DAOPOE010000141         6047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_14323_length_6047_cov_4.792390, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000141 DAOPOE010000000
VERSION     DAOPOE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14323_length_6047_cov_4.792390"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKM94_13100"
     CDS             <1..555
                     /locus_tag="PKM94_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67614.1"
                     /translation="AAPAGTTAGETAAPGAPSPSSVQSEQVSPSEEVISGFIARRQVH
                     REYTRVSGLGALAEIGGAGSVVFSSGLGLMLSGVLIIAFILLCLALPVYTLYNLWAGK
                     GTADEQALQLKRILRYHWALLAIFGLLVFLSFFGGEYGSLAAQKYSSLGDSYGPGVLL
                     GVLSWGLIVALAASILAAAKGAEI"
     gene            600..1358
                     /locus_tag="PKM94_13105"
     CDS             600..1358
                     /locus_tag="PKM94_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD67615.1"
                     /translation="MVKQTIFVALNAIIAFAIGFGLWYGVFRTSETPIVHSIYQGGPL
                     VVLLIMIFIMLIVFVIERYLSLYKVAKGRGSVQVFFKKLVTLIQNDDFDGALAACDKQ
                     RGTTANVLRAGVERYREIKGNAEYTPEKRIQLTQSAIEEANALEGPLMERNLIALSTI
                     ASIATMVGLLGTTIGMIRAFAATGNVEGGVIDAQQLAVGISEALVNTAGGLLSGILGI
                     FFYNFFVNKVDAFNYTTDEATFEVMQILKQKEGI"
     gene            1362..1856
                     /locus_tag="PKM94_13110"
     CDS             1362..1856
                     /locus_tag="PKM94_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD67616.1"
                     /translation="MAKKRRVAIRIDMTPMVDIAFLLLIFYMATTQFKPPEARAVELP
                     KSHSQIELPDKDIINITVSKLDSIYVDYVEKATITIEGQDVTTTVRRVRTADKYNVAQ
                     EILKARAKNIKSLVVIKADQAASFGIMQDIMKSMQENNLERFLIITDQETEAGGMERV
                     IFEG"
     gene            1916..2569
                     /locus_tag="PKM94_13115"
     CDS             1916..2569
                     /locus_tag="PKM94_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOD67617.1"
                     /translation="MAGEVVQREAKGKKKGLRRQKRRIAIRIDMTPMVDIAFLLLIFY
                     MVSTVFSMPQAMEINLPPAEQMDQEIEVKESNLLTIRVDGQGRYWWNLKTPTPDNLPL
                     LLPPDPQRPDTIPYRLWEDTLRGLLVNQNRLNPKLNTLILIHKDGSYADMVDILDEID
                     LIERSWNEYQAKKLRKKLDELSKDEKFSYRYAIGEWMARDDKVQEAAEAQARLGGLL"
     gene            2569..3348
                     /locus_tag="PKM94_13120"
     CDS             2569..3348
                     /locus_tag="PKM94_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD67618.1"
                     /translation="MAETTRSDKGPLAMAAGGRGWSVLYSPYGAFEMKAKYQRNFLFG
                     TLITTSVVALIVIVAAIISSLPEEDYSDVPQVVIKTVADLGPPPSIARKPPQVQVAQP
                     QVAAPKVGIPKPVADEEVVDEDVVIATQEELAEITAPSISEAAGSGDIVVDIQEEDFI
                     PAPEDFVPVEVQPEVISEVKPDYPPLARQAGITGDVYVKALVDEQGNVMKAILAKSSG
                     TQSLDDAALEAAHKRKYRPGIQNNRPIKVWVTYKVEFRLNE"
     gene            3571..4302
                     /locus_tag="PKM94_13125"
     CDS             3571..4302
                     /locus_tag="PKM94_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD67619.1"
                     /translation="MANQANPLYAPYGAYAMKAAYQKNLAAANLLTALAVGAAVLAGT
                     LWPANEEPAPPPDRSRVISMMDLGGLRPQSIVYDVPELAVPQPQRQEAVAVGIPTPVP
                     DEEYAGERDLVMASPREIAENLGRGADAGGATGVIIDTAVREFIPEPTTFVPTEIPPR
                     LVQQYKGDYPRLAEMIGATGTAQVWVLLDEEGKPREARLAQSTGTPALDEAALAWAMK
                     CTYAPGIQNGRPIKLWVTIKYTFEL"
     gene            complement(4463..>6047)
                     /locus_tag="PKM94_13130"
     CDS             complement(4463..>6047)
                     /locus_tag="PKM94_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67620.1"
                     /translation="WRAESVADDIIATAQVVDHSILGQDLASNGAAPGQVLKWDGSTW
                     IPAQDLTGSSLWQNPAHILKPVDPADTLDVGPYVHLPSTWDALFFNGKLNVASNQTAV
                     RIGQPSGGVRSPALSIFAESGWPIYAHVGDTFGVTWLGPNAAIVGSSPDMDGLAGAFF
                     AKGQWPALLAQHWNGGVAFEAYSLNPWDSVCARFAGGRGISVQSSRIRNGYFEALYAS
                     TSPDVIVAVEYSGDSTLKADHIGVKSTARIADYWGVGGRFEGGYIGAQGIVNGGGSNT
                     YQAVKGEAYSTGAGNCYGFHGYAISPGYNYGAYGYASGGYYACGIYGTANSASTNWAG
                     YFVGDIEVTGNIWRSGALSRIDHPLDPDNKYLQHAQVESPEMLNVYSGNVITDASGYA
                     TVTLPDYCEAYNRDFRYQLTVIGDFAQAIIAEKISAGQFTIRTDKPGIEVSWQVTGVR
                     NDLYAQAHPVDVEAAKSSEDRGKYVHPELFGRDAEWRVGPPPPDTAALAAKNRRIDET
                     ARTVAGKPPEPFPARTNNR"
BASE COUNT         1137 a         1812 c         2057 g         1041 t
ORIGIN      
        1 gctgcgccgg ccggaacgac ggccggggag acggcggcgc ccggagcgcc atccccgtcc
       61 agcgtgcaaa gcgagcaggt cagtccctcc gaggaggtca tcagcggatt cattgcccga
      121 aggcaggtcc accgggagta cacgcgcgtg tccggcctcg gggcgctggc ggaaatcggc
      181 ggcgccgggt cggtggtgtt ctcctccggg ctggggctta tgctctcggg tgtgctgatc
      241 atcgctttca tcctcctctg tctcgccctg ccggtgtaca cgctgtacaa tctgtgggcg
      301 gggaagggaa ccgcggacga acaggcgctg cagctcaagc gcattctgcg ctaccactgg
      361 gcgttgctgg cgattttcgg gttgctcgtg ttcctgtcgt ttttcggcgg ggagtacggg
      421 agtctggcgg cgcagaagta cagcagtctc ggcgacagct acgggccggg cgtgctgctg
      481 ggggtgctgt cctggggatt gatcgtcgcg ctggcggcct cgatcctggc ggcggcgaag
      541 ggtgcggaaa tataagtcgg ttggtgagtg tgcaataaaa ggaaacggag gccatttcag
      601 tggttaaaca gacaatcttc gtcgcgctca acgctatcat agcgttcgcc atcgggttcg
      661 ggctgtggta cggggtgttc cggacatcgg aaacgccgat tgtccactcg atctaccagg
      721 gcgggccgct cgtcgttctc ctgatcatga ttttcatcat gctcatcgtg ttcgtgattg
      781 agcgctacct ctcgctctat aaggtggcca agggccgcgg gtcggtccag gtgtttttca
      841 agaagctggt caccctgatt cagaacgacg atttcgacgg tgcgctggcg gcctgcgaca
      901 agcagcgcgg caccaccgcg aacgtgcttc gcgccggcgt cgaacgctac cgcgagatca
      961 agggcaacgc cgagtatacg ccggagaagc gcatccagct cacccagtcg gcgatcgaag
     1021 aggccaacgc cctcgaggga ccgctcatgg agcgtaacct gatcgcgctg tcgacgattg
     1081 cctcgatcgc gaccatggtg ggtctgctgg ggacgacgat cgggatgatc cgggcgtttg
     1141 ccgcgacggg gaatgtcgag ggcggcgtga tcgacgcgca gcagctggcg gtggggattt
     1201 ccgaggccct ggtgaacacg gccggcgggc tgctctccgg tatcctcggg atcttcttct
     1261 acaacttctt cgtcaacaag gtcgacgcgt tcaactacac gacggacgag gcgacttttg
     1321 aagtgatgca gattctcaag cagaaagagg gaatctaagg tatggcgaag aaacggcggg
     1381 tcgctatccg catcgacatg acgccgatgg tggatatcgc ctttctgctg ttgattttct
     1441 atatggcgac gacgcagttc aagccgccgg aagcccgcgc ggtggagctg ccgaagtcgc
     1501 actcgcagat cgagctgccg gacaaggaca tcatcaacat taccgtgtcc aagctggact
     1561 cgatctacgt cgactacgtg gagaaggcga ccatcacgat cgagggtcag gacgtcacga
     1621 cgacggtgcg ccgggtgcgg accgccgaca aatacaacgt ggcgcaggag atcctcaagg
     1681 cgcgcgccaa gaacatcaag tcgctggtgg tgatcaaagc cgaccaggcg gccagtttcg
     1741 gcatcatgca ggacatcatg aagtccatgc aggagaacaa cctcgagcgg ttcctcatca
     1801 ttacggacca ggagacggaa gccggcggga tggagcgcgt catctttgaa ggctgagcca
     1861 agcggcaccg gaacggccgc cggatgcggc gccggaagga gagtgaagag acaatatggc
     1921 cggtgaagta gtacaacggg aagccaaggg caagaagaaa ggtctgcggc ggcaaaaacg
     1981 ccggatcgcg atccgcatcg acatgacgcc gatggtggac atcgccttcc tgctcctgat
     2041 cttctacatg gtctcgaccg tgttctcgat gccgcaggcg atggagatca acctcccgcc
     2101 cgcggagcag atggatcagg agatcgaggt gaaggagtcc aacctgctga cgatccgggt
     2161 cgacggccag ggtcgctact ggtggaatct caagacgccg acgcccgaca acctgcccct
     2221 cttgctgccg ccggatcccc agcgccccga cacgatcccc taccgcctgt gggaggacac
     2281 gctgcgcggg ctgctggtga accagaaccg attgaacccc aaactgaaca cgctgatcct
     2341 gatccataaa gacggcagct acgctgacat ggtggacatt ctcgacgaaa tcgacctgat
     2401 cgaacgttcc tggaacgagt atcaggcgaa gaagctccgg aagaaactgg acgagctgag
     2461 caaggacgag aagttctcct accggtacgc gattggcgag tggatggcgc gcgacgacaa
     2521 agtgcaggaa gcggccgagg cgcaggcccg gttaggaggg ctgttgtaat ggcagagaca
     2581 acgcggtccg acaaggggcc tttggcgatg gcggccggcg ggcgcgggtg gagcgtgctc
     2641 tactctccct acggcgcctt cgagatgaag gccaagtacc agcggaattt cctcttcggt
     2701 acgctcatca ccacctcggt ggtggcgctc atcgtgattg tcgcggcgat catttcgagc
     2761 ctgcccgagg aggattactc cgacgtgccg caggtggtga tcaagaccgt ggccgatctg
     2821 ggtccgcctc cctcgatcgc ccgcaagcct ccgcaggtcc aggtcgccca gccgcaggtg
     2881 gccgccccta aagtgggtat tcccaagccg gtggccgacg aggaagtggt cgacgaggac
     2941 gtggtgatcg cgacccagga agagctggcg gaaatcaccg ccccgagcat ctcggaagcg
     3001 gccggttccg gcgacatcgt cgtcgacatc caggaagagg atttcatccc cgccccggag
     3061 gactttgtcc cggtcgaggt ccagccggag gtcatctccg aggtgaaacc ggattacccg
     3121 ccgctcgccc ggcaggccgg gatcaccggc gacgtctacg tcaaggcgct ggtggatgag
     3181 caggggaatg tcatgaaggc gattctcgcc aagtcctcgg gcacgcagtc gctggacgat
     3241 gcggcgctcg aggcggccca caaacgcaaa taccggccgg ggatccagaa caaccggccg
     3301 atcaaggtct gggtaacgta caaagttgag ttccggctca acgagtagcc gacatcggtt
     3361 ttcactctcc gtttacaggg cgcccgccgg cttcggcggg cgcttctctt ttggccattt
     3421 tccgctcccc gattgccccg gcttctccgt cactctagtg agggcgtccc cgcagacccg
     3481 gccgcccgtt tcagggaaga atcgccggct cggcgggtac tctcgacaga ggcgacggag
     3541 gttcggtatt cgaagtgaga ggagccaagt atggcgaacc aggcaaaccc gctgtacgcg
     3601 ccctacggcg catacgcgat gaaagcggcc taccagaaaa acctggcggc ggcgaacctg
     3661 ctgacggcgc tggcggtcgg cgcggcggtc ctggccggaa cgctctggcc ggcgaacgaa
     3721 gagcccgccc ccccgcccga ccggtcgcgc gtgatctcga tgatggatct cgggggactt
     3781 cggccccaga gcatcgtcta cgacgtgccg gagctcgccg tgccgcagcc gcagcgccag
     3841 gaggcggtgg cggtgggaat cccgacgccg gtgccggatg aggagtatgc gggagagcgc
     3901 gacctcgtga tggcgtcgcc gcgcgagatc gcggagaatc tcgggcgggg cgcggacgcg
     3961 ggcggagcga ccggcgtgat catcgatacc gccgtccgcg agttcatccc cgaaccgacg
     4021 accttcgtgc cgaccgagat cccgccgcgc ctggtccagc agtacaaggg ggactatccc
     4081 cggctggccg agatgatcgg ggcgaccggg accgcccagg tctgggtgct gctcgacgag
     4141 gaggggaagc cgcgggaggc gcgcctggcg cagtcgaccg ggacgccggc gctcgacgaa
     4201 gcggccctgg cgtgggcgat gaagtgcacc tacgcgccgg ggatccagaa cgggcggccg
     4261 atcaagctct gggtgacgat caagtacacg tttgagctgt gaccgattcg ctcgggccgc
     4321 ccctgaacgg ctcacggagc cgggacggca aggatccccc cgtgggccgg tgcagcagtc
     4381 gcagcggccg cggccgccga aagcgagcgc cccccggcga ccagccgggg ggcggttttc
     4441 tatgcggagc cgacgggcgc ggctagcggt tgttggtgcg ggcggggaaa ggttccggcg
     4501 gcttccccgc gacggtgcgg gcggtctcgt cgatcctgcg gttcttcgcc gcgagggccg
     4561 cggtgtcggg cgggggcggg ccgacgcgcc actccgcgtc cctgccgaac aactccggat
     4621 ggacatactt gccgcggtcc tcggacgatt ttgccgcttc cacatcgacc gggtgggcct
     4681 gcgcgtagag gtcgttgcgg acgcccgtca cctgccagga gacttcgatc ccgggcttgt
     4741 cggtacgaat cgtgaactgt ccggcgctga tcttctcagc gatgattgcc tgcgcgaaat
     4801 caccgatcac cgtgagctga tagcggaagt cgcggttgta cgcctcgcag tagtccggga
     4861 gagtaacggt cgcatagccg gaggcgtcgg tgatcacgtt gccgctgtag acgttgagca
     4921 tctcggggga ttcgacctgg gcatgctgga ggtatttgtt gtcggggtcc agagggtggt
     4981 cgatacgcga cagggcaccg ctcctccaaa tgttgccggt gacttcaatg tcgccgacga
     5041 aatacccggc ccaattggtg gaggcgctgt tggccgttcc atatatgccg cacgcgtagt
     5101 agccgccgga ggcatagccg taggccccgt agttgtaacc gggagagatg gcataaccgt
     5161 gaaacccata gcaattgccc gcgccggtcg agtaagcttc gcccttcacg gcctgatacg
     5221 tgttggagcc gcccccgttg actatgccct gggcgccgat gtagccgcct tcgaagcgac
     5281 cgccgacgcc ccagtaatcg gcgatgcggg cggtggattt gacgccgatg tgatcggcct
     5341 taagtgtgga atcgccactg tactcgaccg ccacaatgac gtcgggggag gtgctcgcgt
     5401 aaagggcctc gaagtagccg tttcgaatac gactcgactg gacgctgatc ccgcgaccgc
     5461 cggcgaagcg ggcgcacaca gaatcccagg gattcaagga gtaagcctca aaagccacac
     5521 cgccgttcca atgctgtgcc agaagagccg gccattggcc tttcgcaaaa aaagctcccg
     5581 ccaggccatc catgtcgggg ctgctgccga ctatggcagc gttcggaccc aaccaggtca
     5641 cgccgaaggt gtctccgaca tgggcataga tcggccaccc ggactcggca aagatagaca
     5701 gcgccggaga tctcaccccg ccggagggct gccctatgcg tactgcggtc tgattgctgg
     5761 cgacgttcag tttgccgttg aaaaagagtg cgtcccaggt tgagggcagg tgcacgtacg
     5821 gtcccacgtc gagggtgtcg gcgggatcga ccggcttgag gatgtgggcg gggttttgcc
     5881 acagggagga accggtgaga tcctgggcgg ggatccaggt ggaaccgtcc cacttgagca
     5941 cctggccggg ggcggccccg ttcgaggcaa gatcctggcc caggatcgag tggtcgacga
     6001 cctgggcggt ggcgatgatg tcatcggcca cgctctccgc ccgccag
//
LOCUS       DAOPOE010000142         5979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_14530_length_5979_cov_4.742910, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000142 DAOPOE010000000
VERSION     DAOPOE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14530_length_5979_cov_4.742910"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKM94_13135"
     CDS             complement(<1..1062)
                     /locus_tag="PKM94_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67621.1"
                     /translation="MELLRAEHLSFRYETQSELLLADVSFSLDHAARVGLIGNNGSGK
                     TTLLALIRSELSPVSGSLTVRPDTRTGSLPQEVAFAEDASVEDYLWSARPDLDHLRRR
                     LAAADQSSVDYADLVNLFYQAGGAVTEAAIAHLCAGFRLHPSLLRRPLAVLSGGEKTK
                     VALARLLLLGPDLLLLDEPTNHLEIAALEWLETYLAGLSIPCIVISHDRRFLDVCANE
                     IWELDRGRLTVYSGNYSFYRAAREADRRRRERICEEAQQKAKRLREAAARRRAESERT
                     EHFKFKRSVSKRGSIQKRDAGSGRSGADSSKTMRAALALEKRLAALREQAEAQRPFLE
                     KERRITLACPPLDAPLPLTV"
     gene            1255..2817
                     /locus_tag="PKM94_13140"
     CDS             1255..2817
                     /locus_tag="PKM94_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOD67622.1"
                     /translation="MKFNGREYQPFEGAFPQRTYDERFTGRRHVRTIPHRRDKVTTLD
                     AVMEVIRDGDTISYPHYYRTGDKGLRLVVEALRRHGKRDIRIYGNAFFDHVDPWLIEA
                     VRAGVIGGIYGNVYRKFGQYVTAGELLPWVGVGFSHGDRVRKLQTGEVTVRAAFGPVP
                     IADRWGNANGLMGKPEHLCGPVGLFDADAEYAEYVCLLAGTVSETAVLPAPVSMEQVD
                     FVVPVDDPGLNEGIGSGTLDMNKARTNPFNAQVGENITRVIRAAGVVKDDFSFQVGSG
                     AGLLVLENIRAMLKEMKVRANFSIGGVTSLHVDMLEEGTLYQLMHGQLFEPSPRMFAS
                     MLTHPRHHEITTAYYASVANKEAAVNLLDLAVLSALEVDLEFNLNTVCAGGRIIGGIG
                     GGQDVAAGAALTIIFMPLATGKDGKGFPKVVDRVYTRTTPGEVVDVVVTEEYAAVNPA
                     GSSRCKEAILAEGKRHGVKLVSIEELRALSEKKAREFGTIPPTPPATGEVVHIIEWRD
                     GTLLDVVRKAGK"
     gene            2848..3867
                     /locus_tag="PKM94_13145"
     CDS             2848..3867
                     /locus_tag="PKM94_13145"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOD67623.1"
                     /translation="MIPETAVLARVYRGSAVEAVHYGSAAVVDAAGELVWYAGDPYVK
                     SMTRSAIKPFQTLALLRTGAADHYGFEPRQLAIMCGSHNGSDAHREAVKGNLERAGFT
                     AADLQCGAHWPIGMEQARVFPAHGEERDPLRHNCSGKHSGFLALAKFLGDPPERYLDP
                     AAKGQALVRRALAEVCEYPEAQMPAGIDGCSAPNYPLPLYNLALGFKKLAATEAAEEG
                     LRGALRRVRDAMLAHPEMVSGEGRIDLDLARSFPGRMVCKIGAEGIEGIGLVDPPLGI
                     AIKIADGSWRAMGAICVAIFRQLGLIDDITRFPLLGPHENPAVRNARGIVTGRIAAEV
                     VLKRA"
     gene            4008..5417
                     /locus_tag="PKM94_13150"
     CDS             4008..5417
                     /locus_tag="PKM94_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOD67624.1"
                     /translation="MAKDHPPVEPFRIKSVEPVRLLSRPERERRVVEAHYNVFKIDAA
                     DIYIDLLTDSGTAAMSNRQWAALMMGDESYAGATSFRRFERAVREIFGKRFVIPCHQG
                     RAAENLMFSTVAERGKYIINNTHFDTTRANTLHKGGIPIDLPCREASLDEAAPFKGNM
                     DVPRLEQFIRERGRDRITMVMLTVTNNSVGGQPVSMANIRAAAQVCREHRIPLYFDCA
                     RFAENAYFIKRREPGYAQKPIREIVAEMFSYVDGALMSAKKDALANIGGFITLDDEDL
                     YRRITELMIIVEGFPTYGGLAGRDLDVLAVGLEEVMDFDYLDYRVGQAEYFGELLKEA
                     GMPIVEPAGGHAVFVDAGAFLPHIPAGQFPGQTLAVEFYREGGVRVVEIGSLMFSDVD
                     PETGKVTMAPKELVRLAMPRRVYTNTQYEYIADVAARIAGRKDQLVGMKVVRQPRFLR
                     HFTCDLAPLTPEEARSRRE"
     gene            5414..>5979
                     /locus_tag="PKM94_13155"
     CDS             5414..>5979
                     /locus_tag="PKM94_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67625.1"
                     /translation="MSRRQGLLAAILTVLAVTAAGCGAVRIETGATPEEETARSESQA
                     SALRRTWAQTFDQADRLRRADLLKTHLDSTSARLILVGRQFAGEWRDGNRGRGQVIPA
                     EEFRRVIDNSIAKDRPILQAWEDNLEYGWEWVRDSSRFDSGLIGLFEEFVRHYYDVYS
                     AVFLPQADAETYVAEIDRMEAATRKLSE"
BASE COUNT         1040 a         1693 c         2298 g          948 t
ORIGIN      
        1 gaccgtgagg ggaagagggg cgtcaagggg cgggcaggcc agcgtgatgc ggcgctcctt
       61 ctcaaggaac ggccgctggg cctccgcctg ttcgcgcagg gcggcgaggc gtttctccaa
      121 agcgagggcg gcgcgcatag ttttggacga gtcggccccg cttcgcccag aaccggcatc
      181 ccgcttctga atgctccccc tcttgctgac cgagcgcttg aacttaaagt gttcggtgcg
      241 ttcggattcg gcgcggcgcc gggcagccgc ctcgcgaagc ctcttcgctt tctgctgcgc
      301 ttcttcgcag atccgctcgc ggcggcggcg gtcggcctcg cgcgccgcgc ggtaaaatga
      361 gtagttgccg gagtagaccg tcaggcggcc gcggtcgagc tcccatatct cgttggcgca
      421 gacatcgagg aaacggcgat cgtggctgat gacgatgcag gggatggaca ggccggcgag
      481 gtacgtctcg agccactcga gggcggcgat ctcgagatgg ttggtcggtt catccagcag
      541 cagcagatcg ggcccgagca agaggagacg ggcgagcgcg accttggtct tctcgccgcc
      601 cgagaggacc gccagcgggc ggcggaggag cgacgggtgg aggcgaaaac cggcgcacag
      661 gtgcgcgatc gcagcctccg tgaccgcgcc gcccgcctgg taaaacaggt tcacgaggtc
      721 ggcatagtca accgacgact ggtcggccgc ggcgaggcgg cggcggagat ggtcaaggtc
      781 ggggcgggcc gaccagaggt aatcctcgac ggaagcgtct tcggcaaagg cgacctcctg
      841 gggaaggctg ccggtgcggg tgtcggggcg gacggtcagg ctgccggaga cgggggaaag
      901 ctcgctgcgg atgagcgcca gaagggtggt cttcccggag ccgttgttgc cgatgaggcc
      961 gacgcgggcg gcgtgatcga gggagaaaga gacgtcggca agcaggagct cggattgcgt
     1021 ctcatagcgg aaagagagat gttcggcgcg gagcaattcc atagagttta ttgtcggcgc
     1081 acgctggtgc gggcattcgc ggccgggttt ttccgggtcg cggggcagcc tgtcggaacc
     1141 tgaccggcac gactcacaat acgctgcggc gacggggggc gccagttgac ttttcgcgtt
     1201 ccccgcgtta cccttgcagc aacgtgcaat gaggttcgac ctgaggagag aatcatgaag
     1261 ttcaacggca gagaatacca gccctttgaa ggggcgtttc cccagcggac atacgacgag
     1321 cggttcaccg gacggcgcca tgtcaggacg atcccgcacc ggcgcgacaa ggtcacgacg
     1381 ctggatgcgg tgatggaggt gattcgggac ggcgacacga tctcctatcc ccactactac
     1441 cggaccggcg acaagggtct tcggttggtg gtcgaggcgc tgcggcggca cgggaagcgg
     1501 gatatccgga tctatgggaa cgcgtttttc gatcatgtcg acccgtggct gatcgaggcg
     1561 gtgcgggcgg gcgtgatcgg ggggatctac gggaacgttt accggaaatt cgggcaatat
     1621 gtgacggccg gggagctgct gccctgggtg ggggtcgggt tttcgcacgg cgaccgggtg
     1681 cggaagctgc agaccgggga ggtgacggtg cgggcggctt tcggtccggt gccgatcgcc
     1741 gaccgctggg ggaacgccaa cgggcttatg gggaaacccg agcatctgtg cgggccggtg
     1801 gggttgttcg acgccgacgc cgagtacgcc gagtatgtct gtctcctggc ggggacggtg
     1861 tcggagacgg ccgtgctgcc ggcgccggtt tcgatggagc aggtggattt cgtggtgccg
     1921 gtggacgatc cggggctcaa cgaggggatc ggctcgggga cgctcgacat gaacaaggcg
     1981 cgcacgaacc cgttcaacgc ccaggtgggg gagaatatca cgcgggtgat ccgggccgcc
     2041 ggcgtggtga aagatgattt cagcttccag gtcgggtcgg gggcggggct gctggtgctg
     2101 gagaatatcc gcgccatgct caaggaaatg aaagtgcggg cgaatttctc gatcggcggg
     2161 gtgacctcgc tccacgtgga catgctggag gaggggacgc tctaccaatt gatgcacggg
     2221 cagttgttcg agccgagccc gcggatgttc gcctcgatgc tcacccaccc gcggcaccac
     2281 gagatcacga ccgcctacta cgcctcggtg gccaacaagg aggcggcggt caacctgctc
     2341 gacctcgcgg tgctgtcggc gctggaagtg gacctggagt tcaacctcaa caccgtctgc
     2401 gcgggggggc ggattatcgg cgggatcggc ggcggacagg atgtcgcggc cggggcggct
     2461 ctcacgatca tcttcatgcc gctggcaacc ggcaaggacg gcaaggggtt cccgaaagtg
     2521 gtcgaccgcg tctacaccag gaccacgccc ggcgaggtgg tcgacgtggt ggtgacggag
     2581 gagtacgcgg ccgtcaaccc ggccggctca agccgctgca aagaggccat tctcgccgag
     2641 ggaaaacggc acggagtgaa gctcgtctcg atcgaggaac ttcgcgccct ctcggaaaag
     2701 aaagcgcgcg agttcggcac gatcccgccg acgccgccgg cgaccgggga agtcgtccac
     2761 attatcgagt ggcgggacgg aacgctgctc gacgtggtga gaaaggccgg caagtagcgg
     2821 cggggacgag gccaaagtga ggcggcgatg ataccggaaa cggcggtgct ggcccgcgtg
     2881 taccgcgggt cggcggtaga ggcggtccac tacgggtcgg cggcggtcgt ggacgccgcg
     2941 ggggagctgg tctggtacgc gggggatccc tatgtcaaga gtatgacgag gtcagcgatc
     3001 aagccgttcc agacgctcgc gctgctgagg accggggcgg ccgaccacta cgggtttgag
     3061 ccgcgccagc tggcgatcat gtgcgggtcc cacaacggga gcgacgcgca ccgggaagcg
     3121 gtgaagggca acctggagcg ggcggggttc acggcggccg atctgcagtg tggcgcgcac
     3181 tggccgatcg ggatggaaca ggcgcgggtg ttcccggcgc acggggagga gcgcgacccg
     3241 ctgcgccaca attgttcggg gaagcactcc ggctttctcg cgctggcgaa atttctgggc
     3301 gatcctccgg agcgctacct cgacccggcg gcgaagggac aggcgctggt gcgccgggcg
     3361 ctcgccgagg tgtgcgagta tcccgaggcg cagatgccgg ccgggatcga cgggtgcagc
     3421 gcgccgaact acccgctgcc gctgtacaac ctggcgctgg gattcaagaa gctggcggcg
     3481 acggaggcgg cggaggaggg gctgcggggg gcgctgcggc gggtgcggga cgccatgctg
     3541 gcccaccccg agatggtgtc gggggagggg cggatcgacc tcgacctggc gcgctcgttt
     3601 ccggggcgga tggtgtgcaa gatcggggcg gagggaatcg aggggatcgg cctggtcgac
     3661 ccgccgctgg ggattgcgat caagattgcc gacgggagct ggcgggcgat gggcgccatt
     3721 tgtgttgcga ttttccggca gttggggtta attgacgata tcacgcggtt tccgctctta
     3781 gggccgcacg agaaccccgc ggtgcgcaac gcgcgcggga ttgtcaccgg gcggattgcc
     3841 gcggaggtcg ttttgaagcg agcgtaggca cgtatgcgcg gattcggcta tcgggttccc
     3901 cacctgggac gggactacca ccagcggtgg gtcaagcggg cgacctaacg gcgaccatgt
     3961 caccggatcg ttcacggcag ttttcaccca gaacaaagga gaaggccatg gccaaggacc
     4021 atccgccggt cgagccgttc cgcatcaagt cggtggagcc ggtgcggctg ctgagccggc
     4081 cggagcgcga gcgcagagtc gtcgaggccc actacaacgt tttcaagatc gacgcggccg
     4141 acatctacat cgacctcctg accgattcgg ggacggcggc gatgtcgaac cgccagtggg
     4201 cggcgctcat gatgggcgac gagtcgtacg cgggggcgac ctcgttccgc cggttcgagc
     4261 gggcggtgcg ggagattttc ggcaagcggt ttgttatccc ctgccaccag gggcgggccg
     4321 cggagaacct gatgttctcc acggtcgcgg agcggggcaa gtacatcatc aacaacacgc
     4381 atttcgacac gacccgggcg aacacgctcc acaagggggg gatcccgatc gatctgccct
     4441 gccgggaggc atcgctcgac gaggccgcgc cgttcaaggg gaacatggac gtgccgcgcc
     4501 tggagcagtt tatccgcgag cgggggcggg accggatcac gatggtgatg ctgacggtga
     4561 cgaacaactc ggtgggggga cagccggtgt cgatggcgaa catccgggcg gcggcccagg
     4621 tgtgccggga gcaccggatt ccgctgtatt tcgactgcgc gcggttcgcc gaaaatgcct
     4681 atttcatcaa gcggcgcgaa ccggggtacg cgcagaagcc gatccgggag attgtcgcgg
     4741 agatgttcag ctacgttgac ggggcgctca tgtcggccaa gaaggacgcg ctggcgaata
     4801 tcggcggatt catcacgctg gacgatgaag acctctaccg gcggatcacc gagctgatga
     4861 tcatcgtcga gggattcccg acctacgggg ggctggcggg ccgcgacctg gacgtgctgg
     4921 cggtcgggct ggaggaagtc atggatttcg actacctgga ctaccgggtg gggcaggcgg
     4981 agtatttcgg ggagctgctg aaagaggcgg ggatgccgat tgtcgagccg gccggcgggc
     5041 acgcggtgtt tgtcgatgcg ggggcgttcc tgccgcacat tcccgcgggg cagttccccg
     5101 ggcagacgct ggcagtcgag ttctaccggg aagggggggt gcgggtggtg gagatcgggt
     5161 cgctgatgtt cagcgatgtc gacccggaga cggggaaagt gacaatggcg ccgaaagaac
     5221 tggtgcggct ggcgatgccg cggcgggtgt acacgaatac gcagtatgag tacattgccg
     5281 acgtggcggc gcggatcgcg ggacggaaag accaactggt ggggatgaag gttgtccggc
     5341 agccgcgatt cctgcgccac ttcacctgcg atctggcccc gctgacgccg gaggaggcgc
     5401 ggagccggcg cgaatgagca gacggcaggg cctcctggcg gcgatactca cagtgctggc
     5461 ggtgacggcg gcgggttgtg gcgcggtccg gattgagacc ggggccacgc cggaggaaga
     5521 gacggcgcgc agcgagtcgc aggcgagcgc gctgcggcgg acctgggccc agacgttcga
     5581 tcaggcggac cggctgcgcc gggccgatct gctcaagacc cacctcgatt cgacatcggc
     5641 gcggctcatt ctcgtggggc ggcagttcgc cggcgagtgg cgcgacggca accgggggcg
     5701 cgggcaggtg atcccggcgg aggaattccg ccgggtgatc gacaactcga tcgccaagga
     5761 ccgccccatc cttcaggcct gggaggacaa cctcgaatac ggctgggagt gggtgcggga
     5821 ctccagccgg ttcgacagcg ggctgatcgg gttgttcgag gagttcgtgc ggcactacta
     5881 cgatgtgtac agcgcggtgt ttctgccgca ggcggacgcc gagacatatg tcgcggagat
     5941 cgaccggatg gaagccgcga cgcggaaact gagcgagga
//
LOCUS       DAOPOE010000143         5968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_14563_length_5968_cov_6.243700, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000143 DAOPOE010000000
VERSION     DAOPOE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_5968_cov_6.243700"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKM94_13160"
     CDS             complement(<1..492)
                     /locus_tag="PKM94_13160"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD67626.1"
                     /translation="MKILTRYILAEHIGPFLFAFFTITFLLVIEYVPKIIDHVIDKDL
                     SIWVALELVGLNLAWMLALSVPMSVLVAVLMAFGRLTADFEVTAMKASGINLLRATIP
                     LLIAGGVIAYGMVEFNDKVLPDVNKKARHLWGDISAMRPTLVFRSGIFITDIPGYLVL
                     IDRI"
     gene            591..1787
                     /locus_tag="PKM94_13165"
     CDS             591..1787
                     /locus_tag="PKM94_13165"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOD67627.1"
                     /translation="MSLGVLNRFRRQEPRPDRALRRAVEACDPLAFHRTLPGYCPTPL
                     VSLPGQARRLGLREVLVKDEAHRLGLNAFKVLGASYAVYRFLRRQWEDRQSAPFGDTL
                     ALTPEQRRFFEGATLCTATDGNHGRAVAWSARVVGLPAVIYLPAGSATARVENIRREG
                     ARAEVIDGGYDDAVRQIRNDAAARGWQIISDTAWPGYTEIPAWIQAAYTTLFRELEQT
                     LARPGEPGVDAVIVQGGVGALAAGAAWHYAEQYGARRPRLICVEPVGAACLLASARSP
                     KGTPVPAGNPAPTIMAGLNCREVSVAAWPLIRDTFDAFVAIEDDYAVEAMNRYYRPEP
                     GDPRIIAGESGAAGLAALLALCADPALADLRSALGLGPASRVLLLNTEGATDPVGFAR
                     AVGAQP"
     gene            1784..2326
                     /locus_tag="PKM94_13170"
     CDS             1784..2326
                     /locus_tag="PKM94_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOD67628.1"
                     /translation="MREIFTIGHSRLPFATFLGLLSPHGIAAIVDVRTRPYSRFAPQF
                     NKSRLEAGLKAAGLDYLYFGDRLGGLPEDDRFYDDEGYVRYDRLAASPAFQAGIADLL
                     EAAGRTRLVLMCAEEDPDRCHRRLLIAPELAARGVAVRHLRADGRAQADESGGAPLLL
                     FPVSPAPVRRSPAPVRPRRV"
     gene            2384..3334
                     /locus_tag="PKM94_13175"
     CDS             2384..3334
                     /locus_tag="PKM94_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOD67629.1"
                     /translation="MEAKRISEFAVDDTITAFFSVRKREVREFTRGKFLSLELGDASG
                     RIPAVVWEPDQFALEELAEGMVVKAAGKVTEYNGRLQLTIARIRLAAEDEYRLEDILP
                     HSKQPLEQRRARILTLTRQIDNSHIRRLVESFWEDQAFFARFLAAAAGKLWHHACVGG
                     LSEHSANVGELALRVAAGYDFLNRDYLIFGGLLHDLGKVNTYAGAAAIDYTDEGRLVG
                     HICIADVWVCERAARIEGFPPALLTKLRHLLLSHQGEFETPVRPMMPEAFVLYYCDEI
                     DSKLGAMSRIRERQGKPGWSEYVKLIDRFLYFDDTAQEPR"
     gene            3331..4050
                     /locus_tag="PKM94_13180"
     CDS             3331..4050
                     /locus_tag="PKM94_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67630.1"
                     /translation="MSDNGVILELRRVVRRVEAKTIIDDLSAQFRKGRIYSVIGPSGA
                     GKSSLLRLVNRLDEIGSGEIRFHGLDIRELPPTRLRCRIGYLFQTPYLFPGTVRDNFF
                     SAACARTEETAARLADLVQLEAALIDADTEPLSLGQKQRVALGRLLATEPEIALLDEP
                     TSALDPTITEAIERTVMDIVRRTGLTVIWVTHHPDQALRLGGETLLLVQGRLVESGPS
                     DEVINRPQTDLGRRYRARELR"
     gene            4047..4826
                     /gene="fetB"
                     /locus_tag="PKM94_13185"
     CDS             4047..4826
                     /gene="fetB"
                     /locus_tag="PKM94_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOD67631.1"
                     /translation="MSGANGPGFVQVALALVLVAIAVIVSRYWKLNLAKEMTLGTIRS
                     FVQLIAVGYALEFIFGLNTWWLIVLTILVMLTTGAYTSGGRIRGVDGALAIAWVSIAA
                     GSLVTIAVMVAVDIIPFTARYVIPLGGMIISNAMNAAALAMTRLISDLRAHTLAIETA
                     LALGKPWREASRGLQRDAATAGMLSTLNFMKTVGIVALPGAMTGMILGGADPLEAVLL
                     QIIVAYMLLSAVTITSIVAVDLTVRKFFTPHHQLRRLPALK"
     gene            5075..5162
                     /locus_tag="PKM94_13190"
     tRNA            5075..5162
                     /locus_tag="PKM94_13190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5109..5111,aa:Ser,seq:gga)
     gene            complement(5706..>5968)
                     /locus_tag="PKM94_13195"
     CDS             complement(5706..>5968)
                     /locus_tag="PKM94_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD67632.1"
                     /translation="TPVLLGNYPNPFNPATQIGFSLPAASHVTLVVYNIMGQQVAVLA
                     DGQYEAGDHSVTWDASAQSSGVYLYLLDVSGFSQTRKMMLLK"
BASE COUNT          953 a         2087 c         1942 g          986 t
ORIGIN      
        1 gatccggtcg atgaggacga ggtagcccgg gatatcggtg atgaaaatgc cggagcggaa
       61 caccagcgtc ggccgcatgg ccgagatgtc cccccagagg tggcgcgcct tcttgttcac
      121 atcgggcaga accttgtcgt tgaactcgac catcccgtag gcgatcaccc cgccggcgat
      181 cagaagcggg atggtcgcgc gcaggaggtt gatgccggag gccttcatgg ccgtcacctc
      241 aaaatccgcg gtgagccgcc cgaacgccat cagcaccgcc accagcaccg acatcggcac
      301 cgaaagcgcg agcatccacg ccaggttgag cccgaccagc tccagggcca cccagatcga
      361 aagatccttg tcgatgacgt ggtcgatgat tttcggcacg tactcgatca ccagcaggaa
      421 ggtgatggtg aaaaaggcga acaggaacgg cccgatgtgc tcggccagta tgtagcgcgt
      481 cagtatcttc ataagtgcaa gcaaatatat tggttataca atggaccgcc aagtatgaat
      541 tcgcccgcga cggctttact tccccccgtc ggttgcgtac tatgcctccc gtgagtctcg
      601 gagttctcaa ccgctttcgg cgccaggaac cgcgccccga ccgggcgctg cggagggcgg
      661 tcgaagcctg cgatccgctc gcgttccacc gcaccctgcc cggttactgt ccgacacccc
      721 tcgtgtccct gcccggccag gcgcgccgcc tcggcctgcg cgaagtgctg gtcaaggatg
      781 aggcccatcg cctggggctg aacgctttca aagtcctcgg cgcatcctac gccgtctacc
      841 gcttcctccg ccggcagtgg gaggaccggc agagcgcgcc cttcggcgat acgctggccc
      901 tcacgccgga gcagcggcga ttcttcgagg gggcgacgct gtgcacggcg accgatggca
      961 accacggccg cgcggtggcc tggtcagccc gggtcgtcgg gctcccggcg gttatctacc
     1021 tccccgccgg cagcgcgacc gcccgggtgg aaaacatccg ccgcgagggg gcgcgggcgg
     1081 aggtgatcga cggcgggtac gatgacgcgg tccggcagat ccggaacgac gccgccgccc
     1141 gcggctggca gattatttcc gataccgcct ggccggggta taccgaaatc ccggcctgga
     1201 ttcaggccgc ctacacgacc ctttttcgcg agctcgagca gaccctcgcc cggccgggtg
     1261 agccgggcgt ggacgccgtg atcgtgcagg ggggcgtcgg cgccctcgcc gccggagccg
     1321 cctggcacta cgcggagcag tacggcgccc ggcggccccg actcatctgt gtcgaaccgg
     1381 tcggcgccgc ctgcctgctc gcctcggccc gctcgccgaa ggggactccc gtgccggccg
     1441 gcaaccccgc gcccacgatc atggccggac tcaactgccg ggaagtttcg gtagcggcct
     1501 ggccgctcat ccgcgacacc ttcgatgcct tcgtcgccat cgaggacgac tacgcggtgg
     1561 aggcgatgaa ccggtactac cgtcccgagc cgggcgaccc gaggatcatc gccggcgagt
     1621 ccggcgccgc gggcctggcc gcgctcctcg ccctgtgcgc cgaccccgcc ctggccgatc
     1681 tccgctctgc gctcggcctc ggccccgcct cgcgggtgct gctgctcaat accgagggcg
     1741 cgaccgaccc ggtcgggttc gcgcgcgccg tgggcgccca accatgaggg agatcttcac
     1801 catcgggcac tcgcgcctcc cgttcgcgac gtttctgggc ctgctctccc cccatggcat
     1861 cgccgccatc gtcgacgtcc gcacccgccc ctactcccga tttgcgccgc agttcaacaa
     1921 gtcgcgcctg gaggccggcc tgaaagccgc cggccttgac tacctctatt tcggggaccg
     1981 cctcggcggc ctgcccgagg acgatcggtt ctacgacgac gaagggtatg tgcggtacga
     2041 ccggctggcg gcgtcgcctg cgttccaggc cggcatcgcc gatctccttg aagccgccgg
     2101 ccgaacgcga ttggtgctta tgtgtgcgga ggaagacccc gaccggtgcc atcggcggct
     2161 cctgatcgcc ccggagctgg ctgcccgggg ggtcgcggtg cgccatcttc gggcggacgg
     2221 acgcgcgcag gcggacgaaa gcgggggcgc gccgttgttg ctctttccgg tctcacccgc
     2281 tcccgtgcgc cgctcgccgg cgccggtgcg cccgcggcgg gtttgaacgg ccggccggtc
     2341 gccgccgctt gcgcggcgcg cgccgcgcgc gtatcttggc gccatggagg ccaagcggat
     2401 cagcgaattc gcggttgacg acacgatcac cgccttcttc tccgtacgca agcgcgaggt
     2461 gcgcgagttc acccgcggga aattcctctc cctcgaactc ggcgacgcca gcggccggat
     2521 ccccgccgtc gtctgggaac ccgaccagtt cgctctggag gaactcgcgg aggggatggt
     2581 ggtcaaggcg gccgggaaag tcaccgagta caacggccgc ctccagctca ctatcgcccg
     2641 catccgcctg gcggcggagg acgagtaccg gctcgaggat atcctcccgc attcgaaaca
     2701 gccgctcgag cagcgccggg cccggatcct caccctgacc cggcagatcg acaacagcca
     2761 catccgccgg ctggtcgagt cgttctggga ggaccaggcg ttcttcgccc ggttcctcgc
     2821 cgcggccgcc ggcaaactct ggcaccacgc ctgcgtgggc gggctctcgg aacactccgc
     2881 caacgtcggc gaactcgccc tccgcgtggc cgcggggtac gatttcctca accgcgacta
     2941 cctcatcttc ggcggcctgc tccacgatct cggcaaggtg aacacttacg ccggggcggc
     3001 cgcaatcgac tacaccgatg agggacgcct ggtcgggcac atctgcatcg ctgacgtctg
     3061 ggtgtgcgag cgggctgccc gcatcgaggg attccccccg gccctgctga ccaagctccg
     3121 ccacctcctc ctgtcgcacc agggagaatt cgagacgccg gtgaggccga tgatgccgga
     3181 ggcgtttgtg ctctactact gcgatgaaat cgactccaaa ctcggcgcga tgagccgcat
     3241 ccgcgaacgg caggggaaac cgggctggtc ggagtatgtg aaactgatcg accgattcct
     3301 gtatttcgac gataccgccc aggagccccg gtgagcgata acggcgtcat tctggaactg
     3361 cgccgcgtcg ttcgccgcgt tgaggcaaag acgatcatcg atgacctctc ggcgcagttc
     3421 cggaaagggc ggatctactc ggtcatcggc ccctcggggg cgggaaaatc ctcgctcctg
     3481 cggctggtca accgcctcga cgaaatcggc agcggcgaaa tacgcttcca cggcctcgac
     3541 atccgggagt tgccccccac ccgccttcgc tgccgcatcg gctacctctt ccagacgccg
     3601 tatctctttc cgggcacggt ccgcgacaat ttcttttcgg cggcgtgcgc ccgcaccgag
     3661 gagacggccg cgcggctggc cgatctcgtg cagctcgagg cggcgctgat cgatgccgac
     3721 acagagccgc tctcgctcgg acagaagcag cgtgtggcgc tggggaggct gctggccacc
     3781 gaacccgaga ttgcgctgct ggacgagccg acctcggcgc tcgatcccac catcaccgag
     3841 gctatcgagc gcacggtcat ggacatcgtg cgccgcaccg gcctgaccgt tatctgggtc
     3901 acccaccacc cggaccaggc cctccggctt ggcggcgaga cgctcctgct ggtccaggga
     3961 cggttggtgg agtccggacc gagcgacgag gtgatcaacc ggccgcaaac cgacctgggc
     4021 cggcgctacc gcgcgaggga actgcgatga gcggggccaa cggaccggga ttcgtgcagg
     4081 tggcgctggc gctcgtgctg gtcgccatcg ccgtcatcgt ctcccgctac tggaagctga
     4141 acctggccaa agaaatgacc ctcggcacga tccgctcgtt cgtgcagctc atcgccgtcg
     4201 gttatgccct ggagttcatc ttcggcctca acacctggtg gctgatcgtc ctcaccatcc
     4261 tcgtcatgct gaccaccggc gcgtacacct cgggcggacg gatcagggga gtcgacggcg
     4321 cgctcgcgat cgcctgggtc tcgatcgccg ccggctccct ggtcaccatc gccgtcatgg
     4381 tggccgtcga catcatcccg ttcacggcgc gctacgtcat cccgctcggc ggcatgatca
     4441 tcagcaatgc catgaacgcc gcggccctcg ccatgacccg gctgatctcc gatctgcgcg
     4501 cccacaccct cgccatcgaa accgccctcg cgctcggcaa gccgtggcgg gaggcctcgc
     4561 ggggactgca gcgggacgcg gccaccgccg gcatgctctc cacgctgaat tttatgaaga
     4621 ccgtggggat cgtcgccctg ccgggggcga tgaccgggat gatcctcggc ggcgccgacc
     4681 cgctcgaggc cgtgctcctc cagattatag tcgcctacat gctgctgtcg gcggtgacga
     4741 tcacgtcgat cgtggcggtc gacctgaccg tacgcaagtt cttcaccccc caccaccagt
     4801 tgcgccggct tccggcgctc aagtgaggcc gggcgccgcg ggccgccgcc gatcgaggag
     4861 cgcggcaggg aaaacctgcc cgcaggttct tcgtggaagc gcagccgacc cgcgcgccgg
     4921 ggctgagaat acgcgggggc ggggacttca gtcactacca cggagcgagc acgcgcgcgg
     4981 gggccgaaaa agcttgtctt ttggagcgat tcccctacct tacggcagcc ccgccccggc
     5041 tggtgcagcc ggcggtgggc gctcgttgtg cgacggagag gtggccgagc ggctgaaggc
     5101 gcacgcctgg aacgcgtgta tagggaaacc tatccagggt tcgaatccct gcctctccgc
     5161 catttgtcga ggtctccccg gtttgtctgt cgacaaccac ccggtcgggt cggaccacga
     5221 gatcaaggat tacacccgcg aggcggtccg ggcgcaaggc gagaccgggg gcggccgtgt
     5281 cgccggaagt agtcgcggat ccgcgggacg gaggggccgg ccgatcagcg actatcggtt
     5341 tgaagaggac cgcggtacac gcacgcggcc ggcatccgtc aagacatatg aagaggtggc
     5401 gcagactcat cctcggtgca cctgaaggca gcctgcttgg agtgcaacca agagaatccg
     5461 gccccgactt ccaccttccg caaaagccgg tcgggtagcg cccacctcga cacgggtggc
     5521 gtcgagccta attgagcccg gtcagtaaat cgaggccggc gttgccgccg gccctcggtg
     5581 agttccccca ctgtctctta cctgctgctt atctgcagcg cttgttgtcg ggcatctccc
     5641 ctctggtcgc tcggctccct gcgtttcggc ccgcgacctc ggggtaagct gcctccctcc
     5701 tctgactatt tgagcagcat catcttccgc gtctggctga acccggacac gtcaagaagg
     5761 tagaggtaca caccggagga ctgcgccgat gcatcccagg tgacactgtg gtcgcccgct
     5821 tcgtactggc catcggccag caccgccacc tgctgcccca tgatgttgta gacgacgagc
     5881 gtaacgtgcg acgccgccgg cagcgagaac ccgatctggg tggccgggtt gaacgggttg
     5941 gggtagttgc ccagcaggac gggcgttt
//
LOCUS       DAOPOE010000144         5858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_14931_length_5858_cov_6.608823, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000144 DAOPOE010000000
VERSION     DAOPOE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14931_length_5858_cov_6.608823"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PKM94_13200"
     CDS             <1..1546
                     /locus_tag="PKM94_13200"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOD67633.1"
                     /translation="IRVRETAGAPRTQAALAFDRPIAAAREVNGAEENVGPAEVADGR
                     LVVSLTAFQPKAFAVTLDEGVVTDLLLPWYSPLELPYDGDGVSTDDERCGGDFDGRGY
                     SLAGELLPDTLDYLDIPFVLGPRAAGASNMLTCRGQQVPLPGLGENRLWLLAAAVDGP
                     AVDTFRIGGRALPVELPDYAAPLGQWHNRVVDGQLVESPSAIAPAYLTAAPVAWYGSH
                     RHTPECGNDIYRFTYAFLVSLEVPPGATAVALPNNARIRVLAATVGAKPHGDVTPARP
                     LYDETRATVVKVAADSGAFAGRCRLTLSSPTPGAAIYYTLDGTEPSVRSARYTAPVAV
                     DRTTTLRARAVRDGYDNSYVAERTVHRLELRAPVSVEGLAAGLTARYYEGEWERLPRF
                     DTLTAAAEFTAETVALPPLARQEDFGLLFEGYIRVPADGMYAFSINSDDGSRLYVADT
                     LVVDNDGLHGDTELSGLIGLKAGLHRFTVQMFQGKGGSGLGVSIAGPSLAKQALPAGW
                     LWRPAP"
     gene            2243..5668
                     /locus_tag="PKM94_13205"
     CDS             2243..5668
                     /locus_tag="PKM94_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOD67634.1"
                     /translation="MRKLIVLFGLTALLVPVTSHAQWNNWSDAAYPYQVTISSLPFTV
                     DRDSTLYTVAGDLSTAANGIHINGVSYSKIKGNGRTLTFDGSGTGGHYGVYLHASDHV
                     VVEGLTIMQAATSGQLYGNTGGPNVIGFILGPNNHILVKNCNVHVRGHSSIGFDGAYG
                     DAPFDSWAEGVKTEIEIDGGNFTSYVQSFDNRMWNDAAVMKLDCKPFVQPEQYNFYVH
                     NVVVDSACHTGLLVMDVAWFEYNNVTIDAHNERPSTSWANAHGIGGGNLEAGSRFRYN
                     TVRSGSNHYGGRGMYFDYSVGTAAAPIEIAYNDIRISSGQTTENPAARGIRIRWGCFY
                     LRVHHNYIEVYADDDPATQQISEEAHGIWYTEIGEEGSALGSYNEIYNNTIKTIWSGS
                     SGATPTHVGALMIEQVQDPSKPFGNGCRSYHNRLVSNVHPLMFGELNGGCQEWTSYQD
                     TIEWALPHMTDGYNFSGAVTVGYYNGPSDNNRVIDPVFINAAVNNVVRGGNTGSAYVM
                     RSVELAVKGTNGLPVSGATVHLWPRQAYADSTNLSAAVVAKTTGTNGLVRDTVPYLYR
                     RWSGASLAAESTYNDYRLRVAKGGDITWRRTTLTDPNVNEREWGQFQDTLALPNTAGD
                     GAWGSEEISLWVEGSSQPEGTSLMFIVRASGAAAQNVTYNFATQNGSAQAPGDFTAAS
                     GVGTILAGQTADTISVATVNDAIAESNEAMTFVISNSSQGAITGATAVGTIIDDDTGT
                     DNTPPAAPVLHTPTQGSVLRTTTPVLGVLNSDDRDGDVLTYDFEVYDAVPSLVAGVGG
                     VAEGAATTRWTVPVALQDGAAYTWRARSYDGEEYSPWMSTASFSIDMSSGENNLPTLP
                     VHASPSAGDTLVSAPIVLRVDNAIDPDGDDVTYDFFLYADAGLSELVEEHLGVPETPQ
                     QTAVVLDLTPVDGSTYWWRVRASDGIGATAPTEATSFFYYSLYTGGEDEVPEPVSPGS
                     GATVYTTRPVLKARNAALAGTPAYFFQVASDETFTGIVSASDPVAEDPSGVTEWQVAD
                     DLEVGETYYWRVRADNEAYSAPAAFQVGFEVVAYPNPVHFQQGEVVTITLPDEPIDLL
                     IQSISGETVLIEDGVSGRWTWDGRNASGNLVAIGVYSWFAKGGAYNGKIVVMP"
BASE COUNT         1039 a         1851 c         1968 g         1000 t
ORIGIN      
        1 catccgcgtt cgggaaaccg cgggcgcccc gcggacgcag gcggcactcg ccttcgaccg
       61 accgatcgcg gccgcgcgcg aagtgaacgg cgcggaggag aatgtcgggc ccgccgaggt
      121 ggccgacggc cggctggtcg tgtcgctcac ggcatttcag ccgaaagcgt tcgcagtcac
      181 gcttgatgaa ggagtcgtca ccgacctgct ccttccctgg tattctccgc tcgaactgcc
      241 gtacgacggc gacggggtca gcaccgatga cgagcggtgc ggcggcgact tcgatggtcg
      301 cgggtattcg ctggccgggg agctcctgcc cgacactctc gactatcttg acattccctt
      361 tgtgctcggc ccgcgcgcgg cgggagcgtc caacatgctc acctgccgcg gccagcaagt
      421 gcccttgccg ggactcgggg agaaccgtct ctggctgctc gcggcggccg tggacggccc
      481 ggccgtggac acgtttcgga tcggcggccg cgcccttccg gtggagctgc cggattacgc
      541 cgcgccgctc gggcagtggc acaaccgggt ggtcgatggg cagttggttg agtctccctc
      601 ggcgatcgcc ccggcctatc tcactgcggc gcccgtggcg tggtacggct cgcaccgcca
      661 caccccggag tgcggcaacg acatctaccg cttcacctat gcctttttgg tttcgctgga
      721 ggttccgccg ggggcgaccg cggtggcgct gccgaacaac gcgcgcatcc gggtgctggc
      781 cgcgaccgtg ggggccaagc cgcatggcga cgtgacgccg gcccggccgc tgtacgatga
      841 aacccgcgcc acggtagtga aggtcgcggc cgacagcggc gcgttcgccg gccgatgccg
      901 gttgactctg agttcgccga ctcccggggc cgcgatctac tacacgctgg acggcacgga
      961 gccctcggta cgttccgcgc gctacaccgc gccggtggcc gttgaccgca cgaccactct
     1021 gcgggcgcgg gcggtgcggg acgggtacga caattcgtac gtcgcggagc gcaccgtgca
     1081 ccgcctggaa ctgcgcgcgc cggtgtcggt tgaggggctg gccgccgggc tgacagcgcg
     1141 ctactacgaa ggcgaatggg aacgcctgcc gcgattcgat acgctgacgg ccgcggccga
     1201 atttacggcc gagaccgttg ctctcccgcc gctcgcccgc caggaagatt tcggtctgct
     1261 ctttgaggga tatatccggg tgccggccga cggtatgtat gcgttttcga tcaactccga
     1321 cgacggcagc cggctgtacg tggcggacac gctcgtggtc gacaacgacg gtttgcacgg
     1381 cgacaccgag ttgagcgggt tgatcggttt gaaagccggg cttcaccgct tcacggtgca
     1441 gatgtttcag ggcaaggggg gatccgggct gggggtttcg atcgccggtc cgtcgctggc
     1501 caagcaggct cttccggcgg gctggctgtg gcgcccggcg ccgtagccgg cattgactgc
     1561 gcgagggatc acgtggtggc aaacccgtcc tcggccggac ggtcgaagca gttacagcga
     1621 agggggcagt cgattcgagg gctgtccagg cggctggtgg ttcatccggg ccgaatgtac
     1681 aaggttatgc tcagtcgatg agcttcttgt acgtgcgttg accacgctga cggcactgcg
     1741 ttgcgaacgt ctgcgggcag ccctcgtaag gcgttattgg agagcgcgct gcgcgagtta
     1801 tcggctaccg ggcccggcac ggatattgcg cttgctttcc ggacggaagt tttttgttgt
     1861 agttcgcggt attttggcgt aagatggtct ccgtccaagg gcttcggctc agcgaatggc
     1921 tcttggcccg caagttattc tgccatcggc agttgcaagt gcaagcgtgg ggacatggaa
     1981 actccgggcg aggatcacca ccggttgtag attgaactgt atttgacaac cgaaattacc
     2041 gattaagtca tggagagagg acggattatc gaaccctgat gtgcggctgc ggctaggccg
     2101 ggaaggcggg ggaagtcttc gcgccgcgag gcgaaacggc ctgacacgat actgcagcgg
     2161 ggattgttcc ggatactgct cggcgggtcg gtcccgaacc cggcggctcg ccaccgcgat
     2221 gtcaatagct caggaggcat agatgagaaa gctgattgtc ctgttcggtc ttaccgccct
     2281 tcttgttccc gtcacctcgc acgcgcaatg gaacaactgg agcgatgctg cctaccccta
     2341 ccaagtcact atcagctcac tccccttcac ggtcgatcgg gattccacgc tgtacacggt
     2401 ggcgggcgac ctgtctacgg cggccaatgg aatccacatc aacggcgtca gctactccaa
     2461 aatcaagggc aacggccgga ctcttacgtt tgacggcagc gggaccggcg gccactacgg
     2521 ggtgtacctg catgcctccg accacgtggt ggtggagggc ctcacgatca tgcaggcggc
     2581 cacgtcgggg cagctctacg gcaacacggg aggacccaac gtgatcgggt tcattctcgg
     2641 tccgaataat cacatcctcg tgaagaactg caacgtccac gtgcgcgggc acagctcgat
     2701 cgggtttgac ggcgcctacg gcgatgcgcc gttcgactcc tgggcggagg gggtcaagac
     2761 ggagatcgag attgacggcg gcaacttcac cagctacgtc cagtcgttcg acaaccgcat
     2821 gtggaacgat gcggcggtca tgaagctcga ctgcaagccg ttcgtccagc cggagcagta
     2881 caacttctac gtgcacaacg tcgtggtcga cagcgcctgc cataccggcc ttctggtgat
     2941 ggacgtcgcg tggttcgagt acaacaacgt cacgattgat gcgcacaatg aaagacccag
     3001 cacgagctgg gcgaacgcgc acggtatcgg cggcggcaat cttgaagctg gcagccgttt
     3061 ccggtacaac accgttcgct ccggcagcaa ccactacggc gggcgtggca tgtacttcga
     3121 ttactcggtc ggcacggccg cggccccgat cgagattgcc tacaacgaca tccgcatctc
     3181 cagcgggcaa accacggaga atccggcggc gcgcggtatc cgcattcggt ggggatgttt
     3241 ctacctgcgc gtccaccaca actacatcga agtgtacgcc gatgacgacc cggcgaccca
     3301 acagatatcc gaagaagcgc acggaatctg gtatacggag atcggcgaag agggttcggc
     3361 cctcgggtcg tacaacgaaa tctacaataa cacgatcaag acgatctgga gcgggagttc
     3421 cggcgcgaca ccgacacatg tcggggcgct catgatcgag caggtgcagg atccctccaa
     3481 accgttcggt aacgggtgcc ggagctacca caaccgtctg gtgagcaacg tgcacccgct
     3541 catgttcggc gagctcaacg gcggctgcca ggagtggacc tcctaccagg acacgatcga
     3601 gtgggcgctg ccgcacatga ccgacggata caacttctcg ggagccgtga ctgtcgggta
     3661 ctataacgga ccgtcagaca acaaccgggt gatcgacccc gtgttcatca acgcggccgt
     3721 caacaacgtc gttcgcggcg gcaacaccgg ttccgcgtac gtcatgcgga gcgtggaatt
     3781 ggcggtcaaa gggaccaacg gtctgccggt ttcgggcgcc accgttcatc tgtggccgcg
     3841 ccaggcctat gccgattcca ccaacctgtc ggcggcggtg gtggccaaga ccaccggcac
     3901 gaacggtctg gtgcgcgaca cggtgccgta cctctaccgc cgctggtccg gagcttccct
     3961 ggcggccgag tcgacctaca acgactaccg cctgcgggtg gccaagggcg gcgacatcac
     4021 ctggcggcgc acgacgctga ccgatcccaa tgtcaatgag cgcgagtggg gccagttcca
     4081 ggatacgctg gcgctgccca acacggcggg cgacggggcc tggggatcgg aggagatcag
     4141 cctgtgggtc gagggcagct cgcagccgga ggggacgagc ctcatgttca tcgtgcgggc
     4201 cagcggagcg gcggcgcaga acgtgaccta caattttgcc acgcagaacg ggtcggcgca
     4261 ggcgcccggc gatttcaccg cggcgtccgg ggtcggcacc atcctcgccg gccagacggc
     4321 ggacaccatc tcggtcgcca ccgtgaacga cgcgatcgcg gagagcaatg aggccatgac
     4381 gttcgtgatc tccaattcct cgcagggggc gatcacgggc gcgaccgcgg tcgggacgat
     4441 catcgacgac gacaccggca cggacaacac gccgccggcg gcgcccgtcc tccacacacc
     4501 cacccagggg tcggtgctgc ggacgaccac gcccgtgctg ggcgtgctca acagcgatga
     4561 ccgggacggc gacgtgctca cctatgattt cgaggtgtac gacgccgtac ccagcctggt
     4621 ggccggggtc ggcggggtgg cggagggagc ggcgaccacg cggtggacgg tcccggtggc
     4681 gctccaggac ggagcggcct acacgtggcg ggcccggagt tacgacggcg aggagtattc
     4741 gccgtggatg agcaccgcct cgttcagcat cgatatgagc agtggggaga acaatctccc
     4801 cacgctcccc gtccacgcct cgccgagcgc cggcgacaca ctcgtgtcgg cgcccatcgt
     4861 cctgcgggtc gacaacgcga tcgacccgga cggcgacgac gtgacatatg attttttcct
     4921 ctacgccgat gccggtttga gtgagctcgt cgaagagcac ctcggcgtcc cggagacgcc
     4981 gcagcagacc gccgtcgtgc tcgatctgac cccggtcgac ggatcgacgt actggtggcg
     5041 ggtccgcgcg agcgacggca tcggcgcgac ggcgccgacc gaggcgacct cgttcttcta
     5101 ctacagcctc tacacgggcg gcgaggacga agtccccgaa ccggtcagtc cgggaagcgg
     5161 cgcgaccgtc tacaccactc gcccggtcct gaaggcccgt aacgccgcgc tcgccggcac
     5221 gcctgcgtat ttctttcaag tggcgtccga cgagaccttc accggcatcg tctccgcctc
     5281 ggatccggtg gccgaagatc cttcgggggt gaccgagtgg caggtggcgg atgatcttga
     5341 ggtcggggag acctactact ggcgggtgcg cgccgacaac gaagcctaca gcgcgccggc
     5401 ggccttccag gttggattcg aggtggtcgc gtaccccaac ccggtgcact tccagcaggg
     5461 agaggttgtc acgatcacgc tccccgatga gccgatcgat ctcttgatcc agagcatatc
     5521 gggcgagacc gtgctgatcg aagacggcgt ctccggccgg tggacctggg acggccgcaa
     5581 cgcctcgggc aatctcgtgg cgatcggcgt gtattcgtgg ttcgccaagg ggggcgcgta
     5641 caacggcaag atcgtggtca tgccatagct cgttggccgg ccctgatagg accagggccg
     5701 agggaccccg aagagagcac gctcctcgta tccccattcc ggtaagacac gaactcgaat
     5761 aatccggggc ggcgcgggca gggatagtcc ggcgctcgat gccggtttca ttggcgccgt
     5821 ccggagccgc cgaaaatggc ggaatgcagc cactttcg
//
LOCUS       DAOPOE010000145         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_15428_length_5716_cov_4.596891, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000145 DAOPOE010000000
VERSION     DAOPOE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15428_length_5716_cov_4.596891"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3241
                     /gene="rpoC"
                     /locus_tag="PKM94_13210"
     CDS             <1..3241
                     /gene="rpoC"
                     /locus_tag="PKM94_13210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOD67635.1"
                     /translation="EKRMLQEAVDALFDNGRRTHSVRGDSRRPLKSLSDLLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKNMALELFKPFIIMKLEEKGYVQTVKS
                     AKKLVERERPEVWDILEEIIADHPVLLNRAPTLHRLGIQAFYPVLVEGKAIRLHPLVC
                     AAFNADFDGDQMAVHVPLSFEAQLEAKILMLATNNVLVPSSGRPIAVPSQDMVIGSYY
                     LTKPRQGAKGEGMRFYSPDEALAAYDAGHVDLHATVHVRISGQSVETTPGRLIFNNIL
                     PKNVPYFNETATKGTLGEVVASSFWLEGQAATVEFLDRLKQIGFEYATKAGVTVSIED
                     LVVPDEKAVLIEDARQQVAKIQKQYERGAITYGERYNKVIDLWTHTTNEVGRVMFENL
                     AAQNQGFNPVYMMATSGARGSREQIRQLAGMRGLMAKPQKKLTGGVGEIIENPITSNF
                     REGLSVQEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVIIREIDCGTIRGLV
                     VRAIKEGEEIIESLADRILGRVALEDVFDPYSDELIVEAGQEISERMAMAIEEAGIES
                     VQIRSVLTCESKHGVCAKCYGRNLTTMKMVDIGEAVGVIAAQSIGEPGTQLTLRTFHI
                     GGTAARIAEQSQVVAKYAGTIKLVEIRSLKREDGSVMVVSREGTGEVHIIDEKERVRS
                     RLKVPYGAHLLADDGQAIKKGDIIYEWDPYKTVILAEKNGVVKFRDIEKDVSYREAHD
                     AQTGLIQRIITDTKDKSLFPTILIQSQSGKTLASYRVPTEAQLEVKEGDAVTAGDVLV
                     KMPRVIAKSRDITGGLPRVAELFEARRPHDPAVISEIDGTVEFGRIVRGQQQVLIHGE
                     MDEVREYLVPHGKHMMVHAGDRVTAGDRLCEGAVDPHDILDILGPYEAERYLVNEIQE
                     VYRLQGVRINDKHIEIIVRQMMQKVQIDQVGDTNFLEGEKVDKLKFDEENARVTAEGG
                     EPATSRPLLLGITRASLSTESFISAASFQETTKVLTEAAISGKVDYLMGLKENVIIGH
                     LIPAGTGIDRYKRLEVVTDEAEEEEAARGLIESGHASEGDVADIFDKNS"
     gene            3311..3685
                     /gene="rpsL"
                     /locus_tag="PKM94_13215"
     CDS             3311..3685
                     /gene="rpsL"
                     /locus_tag="PKM94_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD67636.1"
                     /translation="MPTINQLVRKGRRRVIEKTKTPALNSCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNQIEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTM
                     DTAGVNDRRKGRSKYGAKKPKQ"
     gene            3700..4170
                     /gene="rpsG"
                     /locus_tag="PKM94_13220"
     CDS             3700..4170
                     /gene="rpsG"
                     /locus_tag="PKM94_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD67637.1"
                     /translation="MSRRTSAERRPVVPDFKYGEKTITQFISCLMKDGKRSTAQRLFY
                     DAVGQAEKKSGESGLEVFRKALNNVKPVLEVRSRRVGGATYQVPVEVRPERRTALAIR
                     WLISYARARGGHTMAEKLAAEFLAAANNEGAAIKKKEDTHKMAEANKAFAHFRW"
     gene            4423..>5716
                     /locus_tag="PKM94_13225"
     CDS             4423..>5716
                     /locus_tag="PKM94_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD67638.1"
                     /translation="MAHIDAGKTTTTERILFYTGKVHRMGEVDEGAATMDFMEQEKER
                     GITIMSAATTCGWKGHVINIIDTPGHVDFTVEVERSLRVLDGAVALFCAVGGVEPQSE
                     TVWRQADKYSVPRIAYINKMDRTGANFERTLQMMNERFATRCIPVTIPAGEGPMFAGI
                     VDLLSMRFRVFHEDSFGTTFDDLDVPPDMLPAAREYRERLLEAVAEYDDQLLEQFLRD
                     QPLDPAKVMAAVRRATIENHMVPVLCGSSFKNKGIQKLLDCIIDFLPSPLDMPPVQGH
                     DVDHPDKVITRKPSPDEPTAALAFKVVTDPYVGRLTYLRVYSGKVRAGSYLLNPSAGI
                     KERIARILRMHANKREDITEAEAGDIAAVIGMRKTTTGDTLCDQKHPIILERMSFPEP
                     VVSVSIEPKTQADQDKLTNALIKLAEEDPTFKVKQDEDT"
BASE COUNT         1185 a         1658 c         1959 g          914 t
ORIGIN      
        1 cgagaagcgg atgctgcagg aggcggtgga cgcgctcttc gacaacgggc ggcggaccca
       61 ctcggtgcgc ggcgattccc ggcggccgct gaaatcgctg tccgacctgc ttaaagggaa
      121 gcaggggcgg ttccggcaga acctgctggg caagcgcgtg gactattcgg gccgctcggt
      181 gatcgtggtc ggcccggagc tcaagctcca ccagtgcggc ctgcccaaga acatggctct
      241 cgagctgttc aagccgttca tcatcatgaa gctcgaggag aaggggtacg tccagacggt
      301 caagtcggcc aagaagctgg tggagcggga gcgccccgaa gtgtgggata ttctcgagga
      361 gattatcgcc gaccacccgg tgctgctcaa ccgcgccccc acgctgcacc ggctcgggat
      421 ccaggcgttc taccccgtgc tcgtcgaggg gaaggcgatc cgcctgcacc cgctcgtgtg
      481 cgcggcgttc aacgccgact tcgacggcga ccagatggcg gtgcacgtgc cgctgtcgtt
      541 cgaggcccag ctcgaggcga aaatcctcat gctggcgacc aacaacgtcc tcgtgccgtc
      601 atcgggacgg ccgatcgccg ttccctcgca ggacatggtg atcgggtcgt actacctgac
      661 caagccgcgc cagggggcca agggggaggg gatgaggttc tactcgccgg acgaggcgct
      721 ggccgcctac gacgccgggc atgtcgacct gcacgcgacc gtgcacgtgc ggatcagcgg
      781 acaatcggtc gagaccactc ccggacgcct catcttcaac aacattctcc ccaagaacgt
      841 gccgtatttc aacgagacgg cgactaaggg gacgctcggc gaggtggtgg cctccagttt
      901 ttggctggag ggacaggcgg cgacggtgga gttcctcgac cggctcaagc agatcggctt
      961 tgagtacgcg accaaggccg gggtgacggt gtcgatcgaa gacctcgtgg tcccggacga
     1021 aaaggccgtg ctgattgagg atgcgcggca gcaggtggcg aagattcaga agcagtacga
     1081 gcggggggcg attacctacg gcgagcgcta caacaaggtt atcgatctct ggacgcacac
     1141 cacgaacgag gtggggcggg tgatgttcga gaacctggcc gcgcagaacc aggggttcaa
     1201 cccggtgtat atgatggcga cgtcgggcgc ccgcggctcg cgcgagcaga tccgccagct
     1261 cgcggggatg cgcgggctga tggcaaaacc ccagaagaag ctgaccggcg gcgtcgggga
     1321 gattatcgaa aacccgatca cgtcgaattt ccgtgaaggc ctgagcgtgc aggagtactt
     1381 catctcgacc cacggagccc gcaaaggact ggccgacacg gcgctaaaga ccgccgacgc
     1441 cggctacctc acgcgccggc tggtggatgt cgcgcaggat gtcatcatcc gggaaatcga
     1501 ctgcgggacg atccgcgggc tcgtagtgcg ggcaatcaaa gaaggggagg aaataatcga
     1561 gtcgctcgcc gaccgcattc tcggccgcgt ggcgctcgag gacgtgttcg atccttactc
     1621 cgacgaactc atcgtcgagg ccgggcagga gatttcggag cggatggcga tggcgatcga
     1681 ggaggcgggg atcgagtcgg tgcagatccg gtcggtgctc acctgcgaat cgaaacacgg
     1741 cgtgtgcgcg aaatgctacg ggcgcaacct cacgaccatg aagatggtcg acatcggcga
     1801 agcggtcggc gtgatcgccg cccagtcgat cggtgagccg gggacgcagc tgaccctccg
     1861 gacgttccac atcggcggca cggcggcgcg catcgccgag cagtcgcagg tggtggccaa
     1921 gtacgccggg acgatcaagc tcgtggaaat ccggtcgctc aagcgcgagg acggctcggt
     1981 gatggtggtc agccgcgagg gcacgggcga ggtgcacatc atcgacgaga aagaacgggt
     2041 gcgctcgcgc ctgaaagtgc cctacggcgc acacctgctg gccgacgacg gccaggcgat
     2101 caagaagggc gacatcatct acgaatggga tccctacaag accgtgatcc tggccgagaa
     2161 gaacggcgtg gtgaagttcc gggatatcga gaaggacgtg tcctaccgcg aggcccacga
     2221 cgctcagacc gggttgatcc agcgcatcat cacggacacc aaggacaaga gcctgttccc
     2281 gaccatcctg atccagagcc agagcggcaa gacgctggcc agctaccggg tgccgaccga
     2341 ggcccagttg gaggtgaagg agggggacgc ggtgacggcg ggggacgtgc tggtgaaaat
     2401 gccgcgggtg atcgcgaaat cgcgcgacat caccgggggt ctgccccggg tggccgagct
     2461 gttcgaggcg cggcgccccc acgacccggc ggtcatttcc gagatcgacg gaacggtcga
     2521 gttcggccgc attgtccgcg gccagcagca ggtgctcatc cacggggaaa tggatgaagt
     2581 ccgcgagtac ctggttccgc acggcaagca catgatggtg cacgccgggg accgggtgac
     2641 ggcgggcgac cggttgtgcg agggggcggt cgatccgcac gacatcctcg acatcctggg
     2701 gccctacgag gccgagcgct acctggtgaa cgaaatccag gaagtctacc gcctccaggg
     2761 cgtgcgcatc aacgacaagc acatcgaaat cattgtgcgc cagatgatgc agaaagtcca
     2821 gatcgaccag gtcggggaca ccaacttcct ggaaggggag aaggttgaca agctgaaatt
     2881 cgacgaggag aacgcccggg tgacggccga ggggggcgag ccggccactt cgcggccgct
     2941 cctgctgggg atcacgcggg cctccctgtc caccgagagc ttcatctcgg ccgcctcgtt
     3001 ccaggagacg accaaggtgc tgaccgaagc ggcgatttcc ggcaaggtgg actacctgat
     3061 ggggttgaag gagaacgtca tcatcgggca tctgatcccg gccgggaccg ggattgaccg
     3121 ctacaagcgc ctggaggtgg tgacggacga ggcggaggag gaagaggccg cccgtgggct
     3181 gatcgaatcc gggcacgcct cggaaggcga tgtggccgat atttttgaca aaaactcttg
     3241 acaggccggg tggaaagctt acattagcgg tctgtgattt tttggacgat tctgtgtaac
     3301 aggagagatt ttgccgacta tcaaccagct tgttcgtaag ggtcgccggc gggtgatcga
     3361 gaagaccaag acgcccgcgc tcaattcgtg tccgcagaag cgcggcgtgt gcacgcgcgt
     3421 gtatacatcg acgcccaaga agccgaactc ggcgctgcgg aaggttgccc gcgtgcggtt
     3481 gacgaaccaa attgaagtga cggcatacat acctggcgaa ggccacaacc ttcaggaaca
     3541 ctcaatcgtg atgatacgcg gcggtcgtgt caaggatctt cccggggtgc gctaccacat
     3601 catccgcggg acgatggaca cggcgggcgt gaacgaccgc cgcaagggcc gctccaagta
     3661 cggggcaaag aaaccgaagc agtaggactc gttgggctta tgtcgagacg cacgagtgca
     3721 gagagacgac cagtcgttcc cgattttaaa tacggcgaga agacgatcac ccagttcatc
     3781 tcctgtctga tgaaggacgg gaagcggtcc acggcgcagc ggctgttcta cgacgccgtc
     3841 ggccaggccg agaagaagtc cggggagagc ggactggaag tcttccgcaa ggcgctgaac
     3901 aacgtgaagc cggttctgga agtccggtcg cggcgcgtgg gcggcgcgac ctaccaggtg
     3961 ccggttgaag tgcggccgga gcgtcggacg gcgctggcga tccgctggct catttcgtat
     4021 gcgcgcgcgc gcggcgggca tacgatggcg gagaagctgg cggccgagtt cctggccgcg
     4081 gcgaataatg agggcgccgc catcaagaag aaggaggata cgcacaagat ggccgaggcc
     4141 aacaaggcct tcgctcactt ccggtggtag aagcgtcgcc gggctggttg ttttacttgg
     4201 gcctgttgcc gggtccattg aatagggaac gggaattttg acgcgaacaa tgatgtggcg
     4261 gtactgacgc aggcataaca tagccgctcg ccccggccgg cctcccggcc cagggctacc
     4321 gcccctgatg aaccaagcga atgagacagc cgtgagtcag ggattttttt tgtctaaagt
     4381 gcgaatgaca tggacctggc gagagttcga aatataggaa tcatggccca catcgacgcg
     4441 ggcaaaacca cgaccacgga gcgcatcctg ttctacaccg ggaaggtgca tcggatgggc
     4501 gaggtggacg agggcgcggc gaccatggat ttcatggagc aggaaaagga gcgcggcatc
     4561 accattatgt cggccgccac cacctgcggg tggaaaggcc atgtcatcaa tatcatcgac
     4621 acaccgggac atgtggattt tacggttgaa gtggaacggt cgctgcgggt gctggacggg
     4681 gcggtggccc tgttctgcgc tgtcggagga gtcgagccgc agtcggaaac cgtttggcga
     4741 caagcagata agtatagcgt gccgcgcatc gcctacatta ataagatgga ccggaccggc
     4801 gccaacttcg aacgcaccct ccagatgatg aacgaacggt tcgccacccg gtgtatcccg
     4861 gtgaccatcc cggccggcga gggaccgatg ttcgccggaa tcgtcgacct cctctccatg
     4921 cgcttccggg tgtttcacga agactcgttc ggcacgacct ttgacgatct cgatgtgccg
     4981 ccggacatgc tccccgcggc cagggagtac cgggagcggc tcttggaggc ggtggccgag
     5041 tacgacgacc agctcctgga gcagtttctc cgcgaccagc cgctcgaccc ggccaaggta
     5101 atggcggcgg tgcggcgggc gactatcgag aaccacatgg tgccggtgct gtgcgggtcg
     5161 tcgttcaaga acaagggcat tcagaagctg ctcgattgta tcatcgattt cctcccctcg
     5221 ccgctcgaca tgccgcctgt gcaggggcac gacgtcgacc atccggacaa ggtgatcacg
     5281 cgcaagccga gcccggacga gccgaccgcg gcgctggcgt tcaaggtcgt caccgacccc
     5341 tacgtcgggc ggctcaccta cctgcgcgtg tactccggga aggtgcgggc cggctcctac
     5401 ctgctgaatc cctcggccgg gatcaaggag cggatcgcgc gcattctgcg gatgcacgca
     5461 aacaagcggg aagatatcac ggaggccgaa gcgggggata ttgcggcggt gatcgggatg
     5521 cgcaagacga ctaccggcga cacgctgtgc gaccagaagc acccgataat cctggagcgc
     5581 atgtcgttcc cggagccggt ggtgtcggtg tcgatcgagc ctaagacgca ggccgaccag
     5641 gacaagctca ccaacgccct gatcaagctg gcggaagaag atccgacgtt caaggtcaag
     5701 caggacgagg acaccg
//
LOCUS       DAOPOE010000146         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_15716_length_5640_cov_4.454073, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000146 DAOPOE010000000
VERSION     DAOPOE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15716_length_5640_cov_4.454073"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1203
                     /gene="queA"
                     /locus_tag="PKM94_13230"
     CDS             166..1203
                     /gene="queA"
                     /locus_tag="PKM94_13230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOD67639.1"
                     /translation="MDISQFDYHLPPELVAQFPSRRRDESRLMIVDRAGDGCTIVPFR
                     RIADYLEDGEALVVNDTRVFKARLRGNRSSGARVEVFLVRPLDRGDGDAWLALVSPSR
                     RLREGEDVLFGDDRIRLREYQGKGRWAVGFASRAARERIIRRHGRVPLPHYIRREDGP
                     ADLRRYQTVFADRARTGAVAAPTAGFHFTRPLLHDLAGRGVRLVRLTLHVGPGTFKPV
                     SADNIDDHVVDPEWAELPESAARVLNGVRDGGGRITAVGTTSVRTLESVVRGDGRFVP
                     LAGMVDLYIRPGYHFRGVDRLITNFHLPRSSLLILVAAFAGRDRILEAYRYAIAQRMR
                     FYSYGDAMLIL"
     gene            1288..1992
                     /gene="ispD"
                     /locus_tag="PKM94_13235"
     CDS             1288..1992
                     /gene="ispD"
                     /locus_tag="PKM94_13235"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOD67640.1"
                     /translation="MRDRMITAAVIVAGGQSLRFGGEVPKQFREVAGRPLLAWTISRF
                     EAAGSIDRIVVVAAEDYLGYAGEQVVDRYGFAKVQQIVSGGTERRESVWRGLEALPIA
                     TGFVAIHDGARPLVRPADIDRVVGAAKRDRAAILAVPVPDTLKRVRDGYVIATVDRSY
                     LYGAQTPQVFQYDLIMAAHREAAGAAGRYTDDASMVESRGFKVTVVEPSGPNIKITTP
                     EDLVIAEALLAREGHG"
     gene            1985..2473
                     /gene="ispF"
                     /locus_tag="PKM94_13240"
     CDS             1985..2473
                     /gene="ispF"
                     /locus_tag="PKM94_13240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOD67641.1"
                     /translation="MADLRIGHGFDVHAFADDRELVVGGVIIPHERGLAGWSDADVLL
                     HAIMDALLGAAGLPDIGQQFPPGDPAYRQADSAVLLSRVGHLVRSAGYTRILNIDAII
                     MAERPRMNPHIPAMRKAIAEVLRIKEAQVNVKATTTEQLGFVGREEGIAASAVCLIAG
                     DE"
     gene            2466..3095
                     /locus_tag="PKM94_13245"
     CDS             2466..3095
                     /locus_tag="PKM94_13245"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOD67642.1"
                     /translation="MNENPEQIGRYLDYLFTFGPGLVYVTLFAACFMENVFPPFPGDS
                     FIAAAGALVGAGRLDLYLSFALVLAGGMSSVMLMYGVGRRYGREYFLVKDYKYFSAAD
                     ILAFERHLARWGGLLLVCSRFVIGLRSAIALGAGIARYPVRRMAVYSLLSYIVFTAAL
                     FYLAMVVVDNLQRIADYFRTYNMIVWPIVGLATAALVAWKIQQVRTRSK"
     gene            3092..4123
                     /locus_tag="PKM94_13250"
     CDS             3092..4123
                     /locus_tag="PKM94_13250"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD67643.1"
                     /translation="MKVLILAGGDSSERDVSLHSGEAMCKAVRRLGHEVRALDPATGQ
                     SLLDGGGHFALAEAAKTDFRSLAVDAAALLRALAAPDLRDVDIVLLALHGGTGEDGTI
                     QCLLDLAGVRYTGSGMRASAIAMDKALTKRLVSTAGVPTPPFALVRAGQGRSTDEDEA
                     RIRGQFDLPAIVKPNASGSTVGLTRLESWDGLPRAIAQAAQESPEVLIEKYIRGRELT
                     VAVLDGEPLPVVEIRPKKGLYDYEAKYTKGMSEYLAPAPVPDAVRDRLQAEAAAAYRL
                     LGCEGLVRVDYMMAADGSCWFLELNTLPGMTELSLSPMAARAVGIDFPQLIDRIMSSA
                     LKKARRRRG"
     gene            4116..4853
                     /locus_tag="PKM94_13255"
     CDS             4116..4853
                     /locus_tag="PKM94_13255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD67644.1"
                     /translation="MDDLRPKAVLFDLGSTLIEYEAVSWDELSRLCALEAREWLVDEG
                     YAVPEEEEFHVSFERAKAPLRQRAWETLTEWTVPEAARALCAQLEIPYSDELAARFFE
                     EFYRPVDRLLYAYDDAVATLERIRAAVPAVGLISNTVFPEDVHLRELRRFGLSRFFDF
                     TIFSSTFGKRKPHPDIFVQAANQAGYGPAECVYIGDRYVEDIEGPHGVGMPAILKMKA
                     GREYPAEMPLARRRIGALAELADHLRF"
     gene            4953..>5640
                     /locus_tag="PKM94_13260"
     CDS             4953..>5640
                     /locus_tag="PKM94_13260"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HOD67645.1"
                     /translation="MDRTEQLLKEITEANGVSGYEEEVRRLMARELRETADRIEYDRL
                     GSIMGWKKGTADRPRVMVAGHMDEVGFMVREITSEGYIKFLPLGGWWGHVALGQRMRV
                     ITRKGPVIGVVGSKPPHMLEAEERKKVLELKDMFIDVGVQEGFNAKEALGIQLGDPII
                     PDSDFTIMGNPRMYLSKAFDNRMACAVVIDVFRALKGEGHPNTLLGAGSTQEEVGLRG
                     AQTLAHLGDPD"
BASE COUNT          888 a         1746 c         2038 g          968 t
ORIGIN      
        1 ccgctttgcc gcggcgagat tccacccggc gaggatgctc gtttctatcg ccgccgtctt
       61 tcaccgctgt tcaccgccct tatcaatttg tttcgcaggc agccgcggtt gcgtatcatg
      121 ggtggcggcg gccgggaccg gttgcgcccg gcggccggac gccgtatgga tatcagccag
      181 ttcgactacc accttccgcc ggagttggtc gcgcagtttc cctcgcgccg gcgggacgaa
      241 tcccggctca tgattgtcga ccgggccggc gacggctgca cgattgtgcc gttccgccgg
      301 atcgccgact acctggagga cggcgaggcg ctggtggtga acgatacccg ggtattcaag
      361 gcgcggctcc ggggcaaccg ctccagcggc gcgcgggtcg aggtgtttct ggtgcgtccg
      421 cttgacaggg gggatggaga cgcctggctg gcgctagtca gcccatcgcg gaggctccgc
      481 gagggggagg acgtgctgtt cggcgacgat cggataaggc tgcgggaata ccagggcaaa
      541 ggccggtggg cggtgggatt tgcctcccgg gcggcgcggg agaggatcat ccgccggcac
      601 ggccgcgtgc cactgccgca ttacattcgc cgggaggacg gcccggccga tctccggcgt
      661 taccagaccg tgttcgccga ccgcgcgcgg acgggggcgg tggcggcgcc gaccgccgga
      721 tttcacttta cccggccgct cctgcacgat ctggccggcc ggggggtccg tctcgtccga
      781 ctcaccctcc acgtggggcc ggggacgttc aaaccggtgt cggcggacaa catcgacgac
      841 cacgtggtgg acccggagtg ggcggaactg ccggagagcg ccgcccgggt gttgaacggc
      901 gtccgggatg ggggcggccg aatcacggcc gtcgggacaa cctcggtgcg gacgctcgaa
      961 tcggtcgtgc ggggggacgg gcggttcgtg ccgctggccg ggatggtcga tctctacatc
     1021 cggccggggt accacttccg cggcgtggac cgcctgatca ccaatttcca cctcccgcgt
     1081 tcctcgctgc tcatcctcgt cgcggcgttc gccggacggg atcggatcct cgaggcctat
     1141 cggtacgcga tcgcgcagcg gatgcggttc tacagctacg gcgacgcgat gctcatcctc
     1201 tgaattcgct tttgtcggga ccgggcggcg cgtatattcc cgcccgtggc cggacgccat
     1261 ggccggcgtc ccgccgaaac cggaaagatg cgcgaccgca tgatcaccgc agccgtcata
     1321 gtcgccggcg ggcagtcgct ccgcttcggc ggcgaagtcc cgaagcagtt tcgggaagtc
     1381 gccgggcggc cgctgttggc ctggaccatc agccgcttcg aagcggccgg ctccatcgac
     1441 cggattgtgg tcgtggcggc cgaagactat ctcggctacg ccggagaaca ggttgtggac
     1501 cggtacgggt tcgcgaaggt gcagcagatc gtctccggcg ggaccgaacg ccgggagtcg
     1561 gtgtggagag ggctggaagc gctgccgatc gccaccggtt tcgtggccat ccatgacggc
     1621 gcccggccgc tggtccggcc agccgacatc gaccgcgtcg tcggcgcggc gaagcgggac
     1681 cgggccgcca tcctcgcggt cccggtgccc gacacgctca agcgtgtgcg cgacggctac
     1741 gtgatcgcaa ccgtggaccg cagttacctg tacggcgcgc agacgccgca ggtgtttcag
     1801 tacgatctca tcatggcggc gcaccgggag gcggccggcg cggccgggcg gtataccgac
     1861 gatgcgtcga tggtggagag ccgcggattc aaggtgacgg tggtggagcc gagcggaccc
     1921 aacatcaaaa tcacgacccc ggaggatctg gtcatcgccg aagccctcct ggccagggag
     1981 gggcatggct gacctgcgca tcgggcacgg gtttgacgtc cacgcgttcg ccgacgaccg
     2041 cgagctggtg gtgggcggag tgattatccc gcacgagcgc gggctggccg gctggtcgga
     2101 cgccgacgtt ctcctgcacg ccatcatgga tgcgctcctg ggggcggccg gcctgcccga
     2161 tatcgggcag cagttcccgc cgggcgatcc ggcctaccgc caggccgact cggccgtgct
     2221 cctgtcgcgg gtcggccacc tggtgcggtc ggccgggtat acccgcatcc tcaacattga
     2281 cgcgatcatc atggccgagc gccccaggat gaacccgcat attccggcga tgcgcaaggc
     2341 aatcgcggag gtgctgcgga tcaaggaggc gcaggtgaat gtgaaagcca cgaccaccga
     2401 gcaactcggt ttcgtcgggc gggaagaggg gatcgccgcg agcgcggtgt gtctgatagc
     2461 cggcgatgaa tgagaacccc gaacagatcg gccgctacct ggactacctg ttcacgttcg
     2521 gccccgggct ggtgtacgtg acgctgttcg cggcctgctt catggagaac gtgttcccgc
     2581 cttttcccgg cgacagtttc attgcggcgg ccggggcgct ggtcggggcg gggcggctgg
     2641 atttgtacct gagtttcgcg ctagtgctcg cgggggggat gagttcggtc atgctcatgt
     2701 acggtgtggg ccgccgctac ggccgcgagt atttcctggt caaagactac aagtatttct
     2761 cggcggcgga catcctggct ttcgaaaggc acctggcgcg ctgggggggg ctccttctgg
     2821 tgtgctcgcg gttcgtgatc ggcctgcgga gcgctatcgc gctgggggcg ggcatcgccc
     2881 gctacccggt ccggcgcatg gcggtctact cgctgctgtc ctacatcgtg ttcacggcgg
     2941 ccctcttcta cctcgccatg gttgttgttg ataacctcca gcggattgcc gactatttcc
     3001 ggacgtacaa catgattgtt tggccgatcg tggggctggc gacggctgcc ctggtagcgt
     3061 ggaaaatcca gcaggtgaga acgaggtcga aatgaaagtt cttatactcg cgggcggcga
     3121 ctcctccgaa cgcgacgtgt cgctgcactc gggcgaggca atgtgcaaag ccgtccgccg
     3181 cctcggccac gaggtgcgcg ctctggatcc ggccacgggg cagtcgcttc tggacggcgg
     3241 gggacatttc gccctggcgg aggccgccaa aaccgacttc cggtcgctcg cggtcgacgc
     3301 cgccgccctg ctgcgcgccc tggccgcgcc cgatctgcgc gatgttgata ttgtcctctt
     3361 ggcgctccac ggcggaacgg gcgaggacgg cacgatccag tgcctgctcg acctcgccgg
     3421 agtccgatac acgggttcgg gcatgcgggc ctcggcgatt gccatggaca aggcgctgac
     3481 caagcggctg gtgtcgacgg cgggagtgcc gacgccgccc tttgccctcg tgcgcgccgg
     3541 gcaggggcga tcgacggacg aggacgaagc gcgcatccgc gggcaattcg atctgccggc
     3601 gattgtcaag ccgaatgcga gcgggtcgac ggtggggctg acgcggctcg aatcctggga
     3661 cgggctgccc cgggcgatcg cgcaggcggc gcaggaatca cccgaagtgc tcatcgaaaa
     3721 gtatatccgg ggacgcgagc tgacggtggc ggtgcttgac ggcgagccgc tcccggtggt
     3781 cgaaatccgg ccaaagaagg gcctctacga ttacgaggcc aagtacacca agggaatgtc
     3841 cgagtacctg gcgccggcgc ccgttcccga tgcggtgagg gaccgcctgc aggccgaggc
     3901 ggcggcggcc taccgcctgc tcggctgcga ggggctggtg cgggtggatt acatgatggc
     3961 ggccgacggg tcctgctggt tcctggaact caacaccctc cccgggatga ccgagctgtc
     4021 gttgtcgccg atggcggccc gggcggtggg gatcgacttc ccgcagttga tcgaccgcat
     4081 catgtcgtcg gctttgaaga aggcacggcg ccgccgtgga tgacctccgg ccgaaagcgg
     4141 tgctctttga tctcggctcg accctgatcg agtacgaagc ggtgtcgtgg gacgagctct
     4201 cccggctctg cgcgctggag gcgcgcgagt ggctggtcga cgaggggtac gctgtgccgg
     4261 aggaggagga atttcacgtt tcgttcgagc gcgccaaggc gcccctgcgg caacgggcgt
     4321 gggagacgct gaccgaatgg acggttcccg aggcggcgcg ggcgctgtgc gcccagctgg
     4381 agattcccta cagcgacgaa ctggccgccc ggtttttcga ggagttctac cgcccggtcg
     4441 accggcttct ttacgcgtac gacgacgcgg tggccacgct ggagagaatc cgggcggcgg
     4501 tcccggcggt cggactcatc tccaacaccg tgtttccgga agacgtgcac ctgcgggaac
     4561 tccggcggtt cgggctgagc cgcttcttcg atttcaccat cttctcctcc accttcggca
     4621 agcgcaagcc gcaccccgac atctttgtcc aggcggccaa ccaggccggg tacgggccgg
     4681 cggaatgcgt ctacatcggc gaccggtacg tggaggacat cgagggaccg cacggggtgg
     4741 ggatgccggc gattttgaag atgaaagcgg gacgggagta tccggcggag atgccgctgg
     4801 cccgccgtcg gatcggagcc ctggcggaac tggccgacca tcttcgcttt taggggttta
     4861 cgagcagccg cgggccggct atattgtcgc cttgcgggcg caaaccgaag ctgatgacgc
     4921 gagaagaccg ctatagagga aatgaggata tcgtggacag gaccgagcaa ctcctgaaag
     4981 agatcaccga agccaacggc gtgtcggggt atgaggaaga agtgcgccgc ctgatggcgc
     5041 gcgagttgag agagactgcg gatcggatcg agtatgaccg gctgggctcg atcatgggtt
     5101 ggaagaaggg aacggccgac cgcccgcggg tcatggtggc cgggcacatg gatgaagtcg
     5161 ggttcatggt cagggagatc acatccgagg ggtatatcaa gtttctcccc ctgggcggct
     5221 ggtggggtca cgtggccctc ggtcagcgga tgcgggtcat cacccgcaag ggtccggtga
     5281 tcggggtcgt cggctccaag cccccgcaca tgctggaggc cgaggagcgc aagaaagtgc
     5341 tcgagctgaa agacatgttc atcgacgtgg gggtgcagga gggattcaac gccaaggagg
     5401 cgctcggcat ccagctcggc gacccgatca tccccgattc cgatttcacg atcatgggga
     5461 atccgcggat gtatctgtcc aaggcgttcg acaaccgcat ggcctgcgcg gtggtgatcg
     5521 acgttttccg cgcgctcaag ggcgaggggc acccgaacac gctcctcggc gccggttcga
     5581 cgcaggagga ggtcgggctg cggggggcgc agacgctcgc ccacctgggc gaccccgacg
//
LOCUS       DAOPOE010000147         5504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_16213_length_5504_cov_4.539549, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000147 DAOPOE010000000
VERSION     DAOPOE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16213_length_5504_cov_4.539549"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..535)
                     /locus_tag="PKM94_13265"
     CDS             complement(29..535)
                     /locus_tag="PKM94_13265"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD67646.1"
                     /translation="MDILDFAIQMELDGRAYYRNSADQAANLHVRQIFLTLADEEHRH
                     YHVFKKLKGGEDASPADLAPRGKTVMTLKSIFREMIQEGREKLGGDTKREVWKQAREV
                     EIKSEKMYREAAAATSDPVRKDMLTRIADEEQAHIYMIDNMLQFLADPAGFAASQNYK
                     SFMSWEGR"
     gene            complement(604..1743)
                     /locus_tag="PKM94_13270"
     CDS             complement(604..1743)
                     /locus_tag="PKM94_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD67647.1"
                     /translation="MKDKRNEALARLLLDYSVELRKGEVLYLEIKGKDTLELGKHIIR
                     YATEKGAVPFWYYNDESLLRQWIRAAGDDQFKAQAELHLHLMKQADAYIGLRGSDNPF
                     DTADLPQPQMDKMNNLFYKPVHLEERVKRTKWVVLRYPNNAMAQLAETSQETFEDFYY
                     RVCTADYPKMSAAENLLRDLMAATDQVHIKGPGTDLRFSIKGIPPVKCDGKRNIPDGE
                     VYTAPVRDSVNGTLQYNTPSLYQGVLYKNIAFEFRNGKIVKAACEGDQAKLNKILDTD
                     EGARFIGEFSLGVNPFILHPMKDTLFDEKIAGSFHLTPGQCYDEAPNGNQSSIHWDLV
                     CIQRPDYGGGEIWFDGKLIRRDGVFTDPKLEKAFSAENLKNPQEA"
     gene            1860..2642
                     /locus_tag="PKM94_13275"
     CDS             1860..2642
                     /locus_tag="PKM94_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOD67648.1"
                     /translation="MKATARVFIGTAGFSYKDWLGNFYPQFVPPADFLKFYASKFRTV
                     EIDSTFYRIPSAETVAKWARQTPERFVFAAKFPQTVTHEGPPARRVEDARVFIDTMRG
                     LGDKLGPLLLQFPYSFKPAEKNLLADLLAAVPADIRVSVELRNGAWLSEDDLFARLRQ
                     QNIALCLIDHPWMPRLTTCTADFVYIRFLGDRKKIEEDFSYVRDDRAQELAWWRELID
                     RVAGEGKDLYAYFNNHYSGHAPSTAFRLMEMLDGMAPDDTRP"
     gene            complement(2659..4011)
                     /locus_tag="PKM94_13280"
     CDS             complement(2659..4011)
                     /locus_tag="PKM94_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67649.1"
                     /translation="MKILPVAPAGVALLLYGLLLAPAEDPAAPQRVAVIALGLGGVYL
                     LMLRLARPLSLFGRDRPTLLALAAAALLVRAAAVIGADTTAPAPPDVFRCVWEGKLVA
                     EGYNPYAVPPGSSQGEALADDALYPRMCGHDRASTHPPLAQYLFAAAYLLHSESLHGF
                     GLLSLLAELATLLGVIMLAAPLIEKRPFHSWSILIYAFSPLVMLKFLRSHHVDILAMP
                     FFVFSLVRLRRDDAAGTGLLLALATLVKPGALLFAAVIFMHFVGRKRLFFLAGLVFPG
                     VLLYLPFGPKAGTHIAETIRQFLTQPEFNSSAYRLIAWLSPTEEAARVIAAGLLLAVV
                     GIAAYPLRERVPRVSGRLFIAAAAGVILAPALDAAALVWILPFVVLYRNLPFLVLTAL
                     APPAAHVLAAAGGDSAALAWLPALEHAPFYALLLALLIRSAVRNRQPEEAAVGNGRSG
                     "
     gene            4406..>5504
                     /locus_tag="PKM94_13285"
     CDS             4406..>5504
                     /locus_tag="PKM94_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67650.1"
                     /translation="MTTPRTAIPARVTLLYTNIGRGHPFYLDGITEALIRSQAISLVR
                     RELDVFELSRGAARLGWRAARWLYRRGSSGGATGVLYRLIRRGNNYNRETPALRLLGR
                     DIRAALREDPSPVIVAHPTLVGILRHQPHLIYQHGELVTPDEAVVRGAATVLVPTPEA
                     AEPFRRVGGYAAEQVVVTGLCIEPALIRLAADAYGGRVARYAAAGEPMVGAYFSSGAE
                     PTAHVTRLIAAACSAVLEGGRVILFAQSGGRFARRAARAFARRSIPFATLDSSDLIPT
                     DLPRALIVCHASRREENILTARLFPLFDYFVGPSHERTNWSLGLGLPMFVVGPDIGPF
                     APLNRRLLLQAGTAAALETPMDAHLLGPRLAR"
BASE COUNT          884 a         1778 c         1884 g          958 t
ORIGIN      
        1 cgaggtctag ccccggccgc cagccggcct accggccttc ccagctcatg aagctcttgt
       61 agttctgcga ggcggcgaac ccggccggat cggcgaggaa ctggagcatg ttgtcgatca
      121 tgtagatgtg ggcctgctcc tcatcggcga tccgcgtgag catgtctttc cgaaccgggt
      181 cggaagtcgc cgccgccgct tcgcggtaca tcttctccga cttgatctcc acctcgcggg
      241 cctgtttcca gacctcgcgt ttggtgtcgc cgccgagctt ctcccggcct tcctgaatca
      301 tctcgcggaa gatgctcttg agggtcatga ccgttttgcc gcgcggggcg aggtcggccg
      361 gcgacgcgtc ttcgcccccc ttgagtttct tgaacacgtg gtagtggcgg tgctcctcgt
      421 cggcgagggt gaggaaaatc tgccggacat gcaggttggc ggcctggtcg gcactgttcc
      481 ggtagtacgc acggccgtcg agctccatct gaatggcgaa atcgagtata tccatatgac
      541 ccctgttcct gggtgtgggt ttgtccgtgg ggcagagggc gggggccgga ccggccccgc
      601 cgcctaggcc tcctgcggat tcttgagatt ttcggccgag aacgctttct ccagtttcgg
      661 atcagtgaag acgccgtccc ggcggatcaa tttgccgtcg aaccagatct cgccgccgcc
      721 gtaatcgggc cgctggatgc agacgagatc ccagtggatg gaggactggt tgccgttggg
      781 ggcctcgtcg tagcactggc cgggggtgag atggaacgag ccggcgatct tctcgtcgaa
      841 cagcgtgtcc ttcatcgggt gcaggatgaa cgggttgacg ccgagcgaga actcgccgat
      901 gaagcgcgcc ccctcgtcgg tgtcgagaat cttgttgagc ttggcctggt ccccttcgca
      961 ggcggccttg acgatcttcc cgttccggaa ctcgaaggcg atgttcttgt agaggactcc
     1021 ctggtagagc gacggggtgt tgtactggag ggtgccgttg accgagtcgc ggaccggggc
     1081 ggtgtagacc tcgccgtcgg ggatgttgcg cttgccgtcg cacttgaccg gcggaatccc
     1141 cttgatcgag aaccgcaggt cggttcccgg ccccttgatg tggacctggt cggtggccgc
     1201 catgaggtcg cgcagcaggt tctcggcggc actcattttc gggtagtcgg ccgtgcacac
     1261 gcggtagtag aaatcctcga acgtctcctg ggaggtttcc gcgagctggg ccatggcgtt
     1321 gttggggtac cggaggacaa cccacttggt ccgtttgacg cgctcctcca agtggaccgg
     1381 tttgtagaac aggttgttca tcttgtccat ctggggctgg gggagatcgg cggtgtcgaa
     1441 gggattgtcc gatccgcgca ggccgatgta ggcgtcggcc tgtttcatga ggtggaggtg
     1501 cagctccgcc tgggccttga attgatcatc cccggccgcc cggatccact ggcgcagaag
     1561 ggactcatcg ttgtagtacc agaacggaac ggcgcccttc tcggtggcgt agcggatgat
     1621 gtgtttgccc agctcgaggg tgtctttccc cttgatctcg aggtagagga cttccccttt
     1681 tctcagctcg accgaatagt ccagcaggag tcgggcgaga gcctcattgc gtttgtcttt
     1741 cacggagcct cctttggctt ggccttgatc gtcttttgtc gggagtataa ggaaatccgg
     1801 cggtcggggc aacgggcgaa gcggcgaaac cttttgcccg ccgtccggtt tctagacata
     1861 tgaaagccac cgcgcgggtg tttatcggaa cggcgggatt cagctacaaa gactggctgg
     1921 gaaatttcta cccccagttc gtgccgccgg cggatttcct caaattctac gcctccaaat
     1981 tccggaccgt cgagatcgac tccactttct accggattcc gtcggccgag acggtcgcca
     2041 agtgggcgcg ccagaccccc gagaggttcg tgtttgccgc caagttcccg caaacagtca
     2101 cccacgaggg gccgccggcc aggcgggtgg aggacgcccg cgtcttcatc gacaccatgc
     2161 gcggcctcgg cgacaaactc ggaccgctcc tgctgcagtt tccgtactca ttcaagccgg
     2221 cggaaaagaa tctgctggcc gatctgctgg cagcggtgcc ggccgacatc cgggtgtcgg
     2281 tggagctgcg caacggcgcg tggctgtccg aggacgatct cttcgcccgg ctgcggcagc
     2341 agaacatcgc gctgtgcctg atcgaccacc cctggatgcc ccgcctgacc acctgcacgg
     2401 ctgatttcgt gtacatccgg ttcctcggcg accgcaagaa gatcgaggag gatttcagct
     2461 atgtgcggga cgaccgcgcc caggagctcg cctggtggcg cgaactcatt gaccgggtgg
     2521 cgggggaggg caaggatctc tacgcgtact tcaacaacca ctactccggc cacgccccga
     2581 gcacggcgtt ccggctgatg gagatgctcg acgggatggc gcccgacgac acccggccgt
     2641 gagcatccgc ttccccgcct agccgctgcg cccgttcccg accgccgcct cctccggctg
     2701 ccgattccgc accgctgatc gaatcagcag agcgaggagg agcgcataga agggcgcgtg
     2761 ctccagcgcc ggcaaccacg cgagggcggc cgagtcgccc ccggcggcgg ccagcacatg
     2821 cgcggccggc ggcgccaaag cggtgaggac aaggaagggg agattgcggt agagcacgac
     2881 aaacgggagg atccacacca gggcggcggc gtcgagggcc ggggcgagga tgacgcccgc
     2941 cgcggctgcg ataaacagcc gcccgctgac ccgcggcacg cgctctctca aggggtaagc
     3001 cgcgatcccc accaccgcca gcagaagacc ggccgcgatc acccgcgccg cctcctccgt
     3061 gggggacagc cacgcgatca ggcggtaggc cgagctgttg aattccggct gggtcaaaaa
     3121 ctgccggatg gtctccgcga tgtgtgtgcc cgccttcggt ccaaacggga ggtacagcag
     3181 caccccggga aacaccagtc cagccaggaa gaagagccgc ttgcgcccga caaagtgcat
     3241 gaagatcacg gcggcgaaaa gcagcgcgcc cggtttcacc agcgtggcca gcgccagcag
     3301 aaggcccgtg ccggccgcat cgtcgcgccg caggcggacc aggctgaata cgaaaaaggg
     3361 catcgcgagg atgtcgacgt ggtgagaacg gaggaacttg agcatcacca gcggcgagaa
     3421 agcgtagatg aggatcgacc agctgtgaaa gggacgtttc tcgattagcg gagccgcgag
     3481 catgatcacg ccgagcagcg tcgccagctc ggccagcagc gacaggaggc cgaatccgtg
     3541 cagcgactcg ctgtgcagga gataagcggc agcaaagagg tattgcgcca gcggcgggtg
     3601 cgtcgaggcg cggtcgtgcc cgcacatgcg ggggtagagc gcatcgtcgg ccagcgcctc
     3661 tccttggctg gaacccggcg ggactgcgta cgggttgtag ccctcggcca ccagcttgcc
     3721 ctcccacacg cagcggaaga cgtcgggcgg cgccggcgca gtcgtatcgg ctccgatcac
     3781 cgcagccgcc cggaccagaa gggccgccgc cgcgagcgcc agcagcgttg ggcggtcgcg
     3841 tccgaacagc gacagcggcc gggccaaacg gagcatgagc agatacacgc cccccagtcc
     3901 gagcgcgatc acagctacgc gctgcggcgc ggcaggatcc tccgccggcg ccagcaggag
     3961 cccgtagagc aggagcgcca ccccggccgg cgccacgggg aggatcttca cggagcgcct
     4021 ctcgatccac attcgacaat caggtccata cagtctcgta tcgccgcacc cgcttgcgcg
     4081 cctccgggtc acggcggtgt cgcgggtggc tgcggaggcg accggccggg gcggccggga
     4141 gggccgaccg ctcgattcgc cgggcggcgg cgcccgccag cggtgtcccc ggcgtgcctt
     4201 tgggtcgctg gcgctgacgg ggggcagagt caggtcctgt gccgtaacag cggggggcgc
     4261 ggcgcggttc cgaaagccgg ccgaggccgc gattgactaa agcggccggt tggtgtatgt
     4321 tcggtctcac cgtccggccg cctcccggcc gggcggcggc cacggaatct gaaaatcaat
     4381 cgggggatta tatcgggatg aaccggtgac gacgccacgg acagccatac cggcccgcgt
     4441 tacgctgttg tatacgaaca tcggacgtgg ccaccccttt tacctcgacg gcatcacgga
     4501 ggccctcatc cgttcgcagg cgatcagcct ggtccggcgc gagctcgacg tgttcgaact
     4561 ctcgcggggg gcggcgcgac tcgggtggcg ggcggcccgg tggctctacc ggagaggctc
     4621 ctcgggcggg gcgacggggg ttctctaccg tctcatccgg cgggggaaca actacaatcg
     4681 ggagacgccc gcgcttcgct tgctcgggcg ggatatccgg gccgctctgc gggaggatcc
     4741 cagccccgtg atcgtggctc accccactct ggtcggaatt ctccgccacc agccgcacct
     4801 gatctaccag cacggcgagc tggtcacgcc ggatgaggcg gtggtgcgcg gggcggcgac
     4861 cgtgctggtg ccgacgcccg aggcagcgga accgttccgc cgggtcgggg gctatgccgc
     4921 cgaacaggtg gtggtgacgg ggctgtgcat cgagccggcg ctcatccggc tggccgccga
     4981 cgcctatggc ggacgggtcg cccgctatgc ggcggccggg gagccgatgg tgggggcgta
     5041 cttttcctcg ggggcggaac cgaccgccca cgtcacgcgg ctgatcgcgg ccgcctgttc
     5101 ggcggttctg gagggcgggc gggtgatcct cttcgcccag agcggcgggc ggtttgcccg
     5161 ccgggcggcc cgggctttcg cccgccggtc cattcccttc gccaccctgg actcttccga
     5221 cctgatcccc accgatcttc cccgggcgct catcgtgtgc cacgccagcc ggcgcgagga
     5281 aaacattctc accgcccgct tgtttcccct gtttgactac tttgtcggtc ccagccacga
     5341 acggaccaac tggtcgctcg gtctcgggct gcccatgttc gtggtcggcc ccgacatcgg
     5401 tccgtttgcg ccgctcaatc gccggcttct cctccaggca gggacggcgg cggcgctcga
     5461 gacgccgatg gacgcccact tgctcggtcc gcgactcgcc cggc
//
LOCUS       DAOPOE010000148         5427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_16501_length_5427_cov_5.983805, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000148 DAOPOE010000000
VERSION     DAOPOE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16501_length_5427_cov_5.983805"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKM94_13290"
     CDS             <1..743
                     /locus_tag="PKM94_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD67651.1"
                     /translation="LIRPAEKLLKILQAGTAIAAQEDSLITIGIVPTRPETGYGYIKL
                     GDLYKLQDSIPVHYVSGFTEKPRAAVALDYYYSNKYLWNSGMFIWSARAILEAMRKCQ
                     PDLGRLLDDYSRHIGTPGQTEACRGLYAEANGISIDFAVLEKADNVLTIKADIIWDDV
                     GDWRALERYKQKDRDNCVIVGDAVTHESFETTIYNDGDGLVATLGVSDLIIVKANHIT
                     LVAHRTRSQDIKQLLAKINESEEYRKYL"
     gene            712..1356
                     /locus_tag="PKM94_13295"
     CDS             712..1356
                     /locus_tag="PKM94_13295"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOD67652.1"
                     /translation="MSRKSTGSICSLLALALLLGSGCRWQQRTFGAGAAEAAAPGDTL
                     RFDPLDLPQDREIVPAAHPPAAANRRSETVVVGPDAGAVDAADSAALRAADSTAALGG
                     QVFKVQLFTSKLFAEARQARRVAEEIFDRPVTMDYEVPYFKVRVGAFPDRYQAESYAQ
                     RARTAGYPDAWVVAAAAAVRRAAPLYDETSRLPALGDSTGADTLPAGGGSPDGD"
     gene            1346..1981
                     /locus_tag="PKM94_13300"
     CDS             1346..1981
                     /locus_tag="PKM94_13300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD67653.1"
                     /translation="MATRPRVEEIDPQAPARELINEAVTVLDGGGLVVAPTETRYGLL
                     ARADRKIPADRLARAKRRASEKPISIFVGTIEMITHYAELRPTALRLARIFLPGPLTL
                     VLPAVGSWHPVIAPVGKIGVRVSASPVIQAIMERVEYPVTATSANISGDEENETVEQI
                     VEIFGDEVDLYLDGGPLTGMPSTVIDCTIVPPAVLREGAIDPAEIDRVVRG"
     gene            1978..2484
                     /locus_tag="PKM94_13305"
     CDS             1978..2484
                     /locus_tag="PKM94_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOD67654.1"
                     /translation="MSMHEPFTVLFVCTGNTCRSPMAEAALRTLLQKQRPGAFEVISA
                     GASAATGYPATMYAIEAAKIWNCDLSGHKSRPLTADLIDRAHLIFGMSARHVQEVVRM
                     RPAAKDKTFLLKNFPDPGPTGEEVEDPIGQALDRYNETFLEIGEYLGKHLPEIVKRID
                     EKANAAKD"
     gene            2468..3307
                     /locus_tag="PKM94_13310"
     CDS             2468..3307
                     /locus_tag="PKM94_13310"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOD67655.1"
                     /translation="MPRKTKIQVTVVGMLILAGLAPLIYFTAAQIEGAAAGDSAYVAA
                     ESADSLDRPIDNVAFGVGERLQFDVGYGFINAGSASMEVARLIEYEGRPCYQIQTRAE
                     SNSFFSTFFRVEDRVESITDAIGLYPWRFEKDLREGSYRANRQYTFNHREGAVYYGKD
                     TVAVPRFCQDPLSVLYYVRTQPLEVGKSLYIDNFTDGKLYPLEVRVLEKERVTVKAGT
                     FDCVVVEPILQASGVFKHEGRLKVWLTDDRVKMPVLMKSKVIVGSISAELTDFALGDI
                     DEF"
     gene            3310..4269
                     /locus_tag="PKM94_13315"
     CDS             3310..4269
                     /locus_tag="PKM94_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67656.1"
                     /translation="MAAKKPQPAPADLTRVARIPYARRANKTTIEALGRPMAETDGAD
                     FFESLPGFLKAADLKEFIALVAEARRKGRPFHLLLGAHLIKVGLSPILIDLMRRDIVT
                     GLSFSGAGVIHDLELAFFGGTSEDVQAGLADGSFGMVAETGDLFAGAVALAAANRVGL
                     GAAVGRFITERQAPHRALSLFAEAYRLGRPATVHVGIGTDIVAQHPNFDGAAVGAASH
                     LDFRILCTVCGEIDSGGAIANIGSAVILPEVFLKALTVARNLRGGAGRLVTANFDMIA
                     HYRPAVNVVNRPTALAGKGFNFVGHHEVMIPLLAWGLRRACEQ"
     gene            4287..5177
                     /gene="ftcD"
                     /locus_tag="PKM94_13320"
                     /pseudo
     CDS             4287..5177
                     /gene="ftcD"
                     /locus_tag="PKM94_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
     gene            5181..>5427
                     /locus_tag="PKM94_13325"
     CDS             5181..>5427
                     /locus_tag="PKM94_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOD67657.1"
                     /translation="MAGGGGNAAEVRESFFDQVASSAPAPGGGSVSAAAGALAAALAA
                     MVSRLTVGKKAYADVKDELAGVRDRADALRAELEQLIV"
BASE COUNT          970 a         1827 c         1747 g          883 t
ORIGIN      
        1 acctcatccg ccccgccgag aaactgctga aaatcctcca ggccggaacc gccatcgccg
       61 cccaggagga cagcctgatc acgatcggca tcgtccccac ccggccggag accggttacg
      121 gctacatcaa actcggcgac ctctacaagc tccaggacag catccccgtc cactacgtct
      181 ccggtttcac ggagaagccg cgggcggccg tggcgctcga ctactactac agcaacaagt
      241 acctctggaa tagcggcatg ttcatttggt cggcgcgggc gattctcgag gcgatgcgca
      301 agtgccagcc ggatctcggc cggctcctcg acgactactc ccgccatatc gggacgcccg
      361 ggcagaccga ggcgtgccgc gggctctacg ccgaggccaa cggcatctcg atcgatttcg
      421 ccgtgctcga gaaagccgac aacgtcctga caatcaaggc cgacattatc tgggacgatg
      481 tcggcgactg gcgggcgctc gagcggtaca aacagaaaga tcgcgacaac tgtgtcatcg
      541 tcggcgacgc ggtcacccac gagtctttcg aaacgacgat ctacaacgac ggtgacggtc
      601 tcgtggccac gctcggcgtg tccgacctca tcatcgtgaa ggcgaaccac atcactctgg
      661 tcgcgcaccg gacgcggtcg caggacatca agcagctgct ggcgaagatt aatgagtcgg
      721 aagagtaccg gaagtatctg tagcctgctg gcgctggcct tgctgctcgg ctccggctgc
      781 cgctggcagc agcggacgtt cggggcgggg gccgcggagg cggcggcgcc gggcgacacc
      841 ctgcgtttcg acccgcttga ccttccgcag gaccgggaga ttgtgccggc cgcgcacccg
      901 ccggcggccg ccaaccggcg cagcgaaacg gtggtggttg ggccggatgc cggcgccgtc
      961 gacgccgccg acagcgccgc ccttcgcgcc gccgacagca ccgcggcgct cggcggccag
     1021 gtgttcaagg tgcagttgtt cacgagcaag ctgttcgccg aggcccgcca ggcgcggcgg
     1081 gtggccgagg aaattttcga ccgccccgtc accatggact acgaagtgcc gtatttcaag
     1141 gtgcgggtcg gcgcgttccc ggaccggtac caggcggaga gctacgccca gcgcgcccgc
     1201 accgcgggtt acccggatgc ctgggtggtg gcggcggccg cggcggtgcg ccgggccgcg
     1261 ccgctctacg atgaaaccag ccggctgccc gcgcttggcg actcgaccgg cgccgatacg
     1321 ctgccggccg gcggggggtc gccggatggc gactaggccg cgggtggaag agatcgaccc
     1381 gcaggcgccg gcgcgggaat tgatcaacga ggcggtgacg gtgctggacg ggggcgggct
     1441 ggtggtggcg ccgaccgaaa cgcgctacgg tctgctggcg cgcgccgacc gcaagatccc
     1501 cgccgaccga ctggcccgcg ccaagcgccg cgcctccgag aaaccgattt cgatctttgt
     1561 cggaactatc gagatgatca cccactatgc cgaactgcgg ccgaccgccc tgcgcctcgc
     1621 gcgcatcttt cttccgggtc cgctcaccct cgtgctcccg gccgtgggat cctggcaccc
     1681 cgtcatcgcc ccggtcggca agatcggcgt gcgcgtctcc gcctcgccgg tcatccaggc
     1741 gatcatggaa cgggtcgagt atccggtcac ggcgacctcg gccaacatct ccggcgacga
     1801 agagaacgag acggtcgagc agattgtgga aatctttggc gatgaagtcg atttatacct
     1861 ggacggcggt ccgctgaccg ggatgccctc gaccgtaatc gactgcacca ttgtgccgcc
     1921 ggcagtgctc cgggaagggg cgatcgaccc ggcggagatc gaccgcgtag taaggggatg
     1981 agcatgcacg aaccatttac cgttctgttt gtctgtacgg gcaacacctg tcgctcaccg
     2041 atggcggaag cggcgttgcg cacattgctg cagaagcagc ggccgggcgc atttgaggtg
     2101 atctcggccg gcgcgtcggc ggcaactggc taccccgcaa cgatgtacgc gattgaagcg
     2161 gcgaaaatct ggaactgcga cctctccgga cacaaatcgc gacccctgac cgccgacctg
     2221 atcgaccgcg cacacctcat cttcggcatg tccgcccgcc atgtccagga agtggtccgg
     2281 atgcgcccgg cggcgaagga caaaaccttc ctgctgaaaa acttccccga ccccggcccg
     2341 accggggagg aagtcgaaga tcccatcggt caggcgctcg accgctacaa tgaaaccttc
     2401 ctcgaaatcg gcgagtattt gggcaaacac ctgccggaaa tagtaaaaag gatcgacgag
     2461 aaggccaatg ccgcgaaaga ctaaaatcca ggtgactgtc gtgggaatgc tcatcctcgc
     2521 ggggctcgcc ccgctgatct atttcaccgc cgcccagatt gaaggggccg cggccggcga
     2581 ctccgcctac gtcgcggccg aatccgccga ttccctcgac cggccgatcg acaacgtcgc
     2641 tttcggcgtc ggcgaacgcc ttcagtttga tgtcggttac ggtttcatca acgccggatc
     2701 cgcctccatg gaagtggccc gcctcattga atacgagggc cgcccctgct accagatcca
     2761 gacgcgggcg gagtcgaaca gcttcttctc gaccttcttc cgcgtcgagg accgggtgga
     2821 atcgatcacc gacgccatcg gcctgtaccc ctggcgcttc gagaaggatc tccgcgaggg
     2881 gagctaccgg gcgaaccgcc agtacacgtt caaccaccgc gagggcgccg tctactacgg
     2941 caaagacacc gtcgccgtcc cgcgcttctg ccaggatccg ctgtcggttc tctactacgt
     3001 gcggacgcag ccgctcgagg tgggcaagtc gctgtatatc gacaatttca ccgatgggaa
     3061 gctctatccg ctcgaggtgc gggttctgga gaaggagcgg gtcacggtga aagccggcac
     3121 tttcgactgc gtggtcgtcg aaccgatcct ccaggcctcg ggcgtgttca agcatgaggg
     3181 gcggctgaaa gtgtggctga ccgacgaccg cgtgaagatg cccgtgctca tgaaatccaa
     3241 agtgatcgtc gggtccatca gcgccgaact gaccgacttc gcgctcggcg acatcgacga
     3301 attctgatca tggccgccaa gaagccacag cccgccccgg ccgatctgac gcgcgtcgcg
     3361 cgcattccct atgcccgccg ggccaacaag acgaccatcg aggcgctggg acggccgatg
     3421 gccgagaccg acggcgccga tttcttcgag tccctgcccg ggttcctcaa ggcggccgat
     3481 ctcaaagaat tcatcgccct cgtcgccgag gcccgccgga aaggccggcc tttccacctg
     3541 cttctgggcg cccacctgat caaagtcggc ctctcgccga tcctcatcga cctgatgcgc
     3601 cgggacatcg tcaccggttt gtcgttcagc ggggcgggcg tcatccatga tcttgagctg
     3661 gcgttcttcg gcgggacctc cgaggatgtg caggcggggc tggccgacgg cagtttcggc
     3721 atggtcgccg agacggggga cctcttcgcc ggggcggtgg cgctggcggc ggcgaaccgg
     3781 gtcgggctgg gcgccgcggt ggggcggttc atcaccgagc ggcaggcgcc gcaccgcgcc
     3841 ctgtctctgt ttgccgaggc ctaccggctg ggccggccgg ccaccgtcca cgtcggcatc
     3901 ggcaccgaca tcgtcgccca gcaccccaat ttcgacggcg cggcggtcgg ggcggcctcc
     3961 catctggact tccgcatcct ctgcactgtc tgcggcgaaa ttgactccgg cggggctatt
     4021 gcaaatatcg ggtcagcggt tatattgccg gaagtttttc tcaaggcgct gaccgtggcc
     4081 cgcaacctcc gcggcggcgc cgggaggctc gtgaccgcca acttcgacat gatcgcgcac
     4141 taccgtccgg cggtaaacgt ggtgaaccgg ccgaccgcgc tcgcgggcaa agggttcaac
     4201 ttcgtcggac accatgaagt gatgatcccc ctgttggcgt ggggcctccg ccgagcctgc
     4261 gaacagtaac gacgacggag ttggtaatgg ccaagctggt ggaatgcgtt ccgaatttct
     4321 ccgaagggcg gcggcccgaa gtcatcgatg cgatctgcgc cgccatcacc tcggttgacg
     4381 gggtcgtgct tctcgacaag gaaatggaca aggaccacaa ccgtgccgtg gtgaccttcg
     4441 tctgccaccc ctccagggcg gtcgatgcgg ccttcgaggg atacaggata gcggccgagc
     4501 tgatcgacat gcgcacccac cagggggaac acccccgcat gggcgcctgc gacgtctgcc
     4561 ccttcatccc gatctccgag atggaaatcg aggaggccgt cgagctggcc cacaagcttg
     4621 gcaaacgggt cggcgaggaa ctgcagatcc cggtgtacct ctacgaggac gcccgcaccc
     4681 ggccgagccg caagaatctc gccaacgtcc gccaggggga atacgaaggc atccgcgaca
     4741 ctatcgccac cgacccggaa cggcagcccg actacggccc ggcgcagatg aatctccgcg
     4801 ccggcgccac cgccatcggc gtccgtttcc cgctggtcgc gttcaacgtt tatctcggca
     4861 ccaacaacaa gaccgtggcc gaccggattg ccgatgccgt gcgctcgctc accggcggct
     4921 atcgcttcgt caaagccctc ggtttcgaca tcaaggcgcg caaccaggtc cagatctcga
     4981 tgaacctggt gaactacacg aaaaccccca ttttccgggt gttcgagaca atcaagtcgg
     5041 aggccgaccg ctacggcgta ccggtcacgt cgtcggagat catcggcctg gtgcccaacg
     5101 acgccatcct cgcggtcgcc gacttctacc tccgcctgga gaatttctcc aagaaccaga
     5161 tcctcgagga gaagctcaag atggcgggcg gcggcgggaa cgccgccgag gtccgggaga
     5221 gcttcttcga ccaggtggcc tcgtcggccc cggccccggg cggcggttcg gtctcggccg
     5281 cggcgggcgc gctggcggcc gcgctggcgg ccatggtgtc ccgcctcacg gtcggcaaga
     5341 aggcctacgc cgacgtcaag gacgagctcg ccggggtgcg cgaccgggcc gacgccctcc
     5401 gcgccgaact cgagcagctc atagtcg
//
LOCUS       DAOPOE010000149         5324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_16922_length_5324_cov_4.923894, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000149 DAOPOE010000000
VERSION     DAOPOE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_5324_cov_4.923894"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKM94_13330"
     CDS             complement(<1..535)
                     /locus_tag="PKM94_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67658.1"
                     /translation="MKTLSRVFWVALLWAVPTIGLADIQGDTPAEANDKTFDLSALEA
                     FLPAELAGYTASEVHHSGWDSTGLGATTPAEMVTRTFTRQTADSQLVTLTVSFVSSQV
                     LRPAFDLLFVPIDSAVVKPNKEIRDVTVRDQPARLVVKTNEDGSVEKIVLLAELKGGV
                     LAAVKATGPATADEVLAL"
     gene            complement(719..1300)
                     /locus_tag="PKM94_13335"
     CDS             complement(719..1300)
                     /locus_tag="PKM94_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67659.1"
                     /translation="MNLIRLAVIGLMAAPAVLPLAAQQSADKTSKPSGALAWETLAEF
                     LPESIPGYTAGEPTGGSRAVEDPASPERSITHSSVRRSYIRETAEGEQSFINVTIVDA
                     GRSRLVLDPFLPELSFVPSDDRQTVEIKGRRATQIIEYGESDIALGLVYVLVEERLAV
                     IVQGEGVRDMDEVRGVAGAIDFEGLEKAMKAKK"
     gene            1429..3636
                     /locus_tag="PKM94_13340"
     CDS             1429..3636
                     /locus_tag="PKM94_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD67660.1"
                     /translation="MSRLLDDLNDAQREAVQTTEGPLLIIAGAGSGKTRVLTRRLAYI
                     LTRRLAQPHEILAVTFTNKAAGEMKERVAALLGGAMMDMNVSTFHSFCARFLRREGGA
                     IGYRGDFTIFDAADSETLMKSCIKALNLPVSQFAPKAQLRKVSNAKNRFQSAEGFARS
                     AGGYFEQRTAQLYSLYQARLRECNAMDFDDLLLNTVALLQQHSEIGERYRSRFRYLLV
                     DEYQDTNHVQYLLLKSLVGPHRNICVVGDEDQSIYGWRGADIRNILDFEHDFPGAKVI
                     KLEMNYRSTDVILAAASAVIAHNVARKDKVLRTTRRGGEKISLLLVDSAEEEAERVID
                     LVVRDRSRTPLSEMVILYRTNAQSRAFEEQLRRRAIPYQIVGGISFYQRKEIKDLLAY
                     LKLIVNPKDDISFGRIVNYPKRGLGDKSLADLGELTAREGKSLYETATLPDPDLSGLG
                     ARAVRQLRSFTALIERYREQQDAVSTDLLVGDLIRDLGLLEAIRAEEEEIVAATRIEN
                     LEAFIGGVADYTRHRPHATLAEYLAEISLFTDIDEYNESEDKLALMSVHSAKGLEFDT
                     VFIVGLEEGLFPLARTVGEEMELEEERRLFYVGSTRARHKLYLASATARFRFGQSVSI
                     PSRFIKEVPDHLIERTDFRTNRHYELVSAGDRPATARDLPLGRLPAEEGVHYEYEEEE
                     ALRVGRLVRHPTFGRGKVLKVEGRGESLMLEIHFQGLGVKRVMAKYARLTVIG"
     gene            4007..4615
                     /locus_tag="PKM94_13345"
     CDS             4007..4615
                     /locus_tag="PKM94_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD67661.1"
                     /translation="MTDGKAKTGDDKRKELEKRRAFEKEALPHMDALYRTALRMTKNN
                     NDAEDLVQEAYVKAYRFWDRFEPGSNCRAWLFKIMTNIFINDYRSKSRSPMSVNVDEI
                     DDSFLYGQLAATAPSDNPEKELFAKIFDDDVKRAIEGLPEDFRLVVVLSFLEGFSYQE
                     IADIADLQLGTVKSRLHRGRKLLQKALYDYAIKNGYIKDTAR"
     gene            4612..>5324
                     /locus_tag="PKM94_13350"
     CDS             4612..>5324
                     /locus_tag="PKM94_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOD67662.1"
                     /translation="MNCQEALNLLYDIIDKEASEIDTRQVREHLERCSDCFEKYRLQT
                     EIQNFLNEKIRHGQVEVAHERLKSRILDRLDALDGAGEAADRGHTKARGNPRPFRRAA
                     ISLMAAAAALILIGAGMLGVRLYSHQTKFAPLQQAHVNLAAAVREGGSYDAARAAAGA
                     REKFGYQIRPAVGEFHLIAGRSETIMGEQMLHFVYALDRQTVSVFLAPAAWFAQVNDG
                     RLSEVVRDNVRFFDHNCRG"
BASE COUNT         1043 a         1746 c         1589 g          946 t
ORIGIN      
        1 ccagcgccag cacttcgtcc gccgtcgccg gtccggtcgc tttgaccgcc gccagcacac
       61 cgcctttcag ctcggcgagc agcacgatct tctcgaccga accgtcctcg ttcgtcttga
      121 ccaccagtcg ggccggctgg tctctcacgg tcacgtcccg gatctccttg ttcggcttga
      181 ccacggcgct gtcgataggg acaaacagca gatcgaacgc cggacggagc acctgcgagc
      241 tgacgaatga gacagtcagc gtgaccagct ggctgtcggc ggtctgcctg gtgaaagtgc
      301 gggtgaccat ctccgccggc gtcgtcgcgc cgagaccggt cgaatcccat ccgctgtggt
      361 gcacctccga ggccgtgtac cccgccagtt cggccgggag aaacgcctcc agagcgctca
      421 ggtcgaaagt cttgtcattc gcttccgcgg gcgtgtcccc ctggatgtcg gccagaccga
      481 ttgtcgggac agcccacagg agggcaaccc aaaaaacacg actcagcgtc ttcatgacat
      541 cactcctcct tgcacgcttg aaaccgattc tgctcatcca cgataacaat ggccgcggag
      601 cggagcgctg tcaagcgaaa tgacggcgct tcacccgccc ccccgagggc ccaaaaaaga
      661 acgcggccga tcttgtgctc ggccgcgccc cgggctttcc gcatgccccg ataaccggtt
      721 acttcttggc cttcatcgct ttctcaagcc cctcgaaatc aatcgcgccg gccaccccgc
      781 gcacttcatc catatcccgc accccttcgc cctgtacaat caccgccaat ctttcctcca
      841 ccaggacata caccaggccg agcgcgatat cgctctcgcc atactcaatg atctgtgtcg
      901 cccggcgccc cttgatctcc accgtctgcc ggtcatccga cggtacaaag ctcaactccg
      961 gcagaaacgg gtcgagcacg agccgactgc gtccggcgtc gacaatcgtc acgttgatga
     1021 agctctgctc cccctcggcc gtctcacgaa tgtagctgcg ccggacgctg ctgtgggtga
     1081 tcgagcgttc gggcgaggcc gggtcctcca cggcccgcga tccgcccgtc ggttcgcccg
     1141 cggtgtaccc agggatgctc tcggggagaa attcggcgag cgtttcccag gccagggcgc
     1201 cggagggttt cgaggtcttg tccgccgact gctgcgcggc caggggcagc accgccggcg
     1261 ccgccatgag gccaatcacg gccaagcgaa ttaggttcat gctttcgtct cctgatccag
     1321 gaagtgtgac tgtcctgtca taaatatgtc tttcgccgtt gcgcccggcc acagaaaaat
     1381 cgtgaaatcc gattgcgccg accccgggcg aacctatatt cgctcggtat gagtcgcctt
     1441 ctcgatgacc tcaacgacgc gcagcgggag gcggtgcaga cgaccgaggg cccgctgctc
     1501 atcatcgccg gggccggttc cggcaagacc cgggtcctga cccgccggct tgcctatatc
     1561 ctgacccggc gactggcgca gccgcacgaa atccttgctg ttacttttac caacaaggcg
     1621 gccggagaga tgaaagaacg ggtggccgcc ctgctcgggg gggccatgat ggacatgaac
     1681 gtctcgacct tccactcgtt ctgcgcccgt ttcctccggc gtgagggcgg ggcgatcggc
     1741 tacagagggg atttcacgat tttcgacgcc gccgattccg agacgctcat gaagagctgc
     1801 atcaaagcgc tcaacctgcc ggtctcgcag ttcgcgccga aagcccagtt gcgcaaggtc
     1861 tcgaacgcca agaaccggtt ccagtcggcc gagggtttcg cccgcagcgc cggcggctac
     1921 ttcgaacagc gcaccgccca actgtactcc ctctaccagg cgcggctgcg cgagtgcaac
     1981 gccatggatt tcgacgacct cctcctcaac accgtcgccc tcctccagca gcacagcgaa
     2041 atcggcgagc gctaccggtc ccgcttccgc tatctcctgg tggacgagta ccaggacacc
     2101 aaccacgtgc agtacctcct gctgaaaagc ctcgtcgggc cgcaccggaa catctgcgtg
     2161 gtcggcgatg aggaccagtc gatctacggc tggcgcggcg ccgatatccg caacatcctc
     2221 gatttcgagc acgacttccc tggcgccaaa gtgatcaaac tggaaatgaa ctaccgctcc
     2281 accgacgtca tcctggcggc cgcctcggcc gtgatcgccc acaacgtcgc ccgcaaggac
     2341 aaggtgctgc gcaccacgcg ccgcgggggg gagaagatca gcctcctctt ggtcgattcg
     2401 gcggaggaag aggccgagcg ggtgatcgat ctggtcgtcc gggaccggag ccgcacgccg
     2461 ctgtcggaga tggtgatcct gtaccgcacc aacgcgcagt cccgcgcctt cgaggaacag
     2521 ctccggcggc gcgccatccc ctaccagatc gtcggcggca tctcgttcta ccagcgcaag
     2581 gagatcaaag acctcctggc ctacctcaag ctgatcgtca atccgaaaga cgacatcagt
     2641 ttcgggcgca tcgtgaacta cccgaaacgc ggcctcggcg acaaaagcct cgccgacctg
     2701 ggcgaactca ccgcccgcga ggggaagtcg ctctatgaga cggcgaccct gcctgacccg
     2761 gatctctccg ggctgggcgc ccgggccgtc cgccaactcc gctccttcac cgccctcatt
     2821 gaacgctacc gggaacagca ggacgccgtc tcgaccgatc tgctggtggg cgatctgatc
     2881 cgcgacctcg gcctgctcga ggcgatccgg gcggaggaag aggagattgt cgccgccacc
     2941 cgcatcgaga acctcgaggc gttcatcggc ggcgtggctg attacacccg gcaccgcccc
     3001 cacgccaccc tcgccgaata cctggccgag atctcgctct tcaccgacat cgacgagtac
     3061 aatgagtccg aggacaaact ggccctcatg tcggtccact cggccaaggg gctggagttt
     3121 gacaccgtgt tcatcgtggg gctggaagaa ggactgtttc ccctcgcccg taccgtcggg
     3181 gaggaaatgg agctggaaga ggagcggcgg ctgttctatg tcggctccac ccgcgcccgc
     3241 cacaagctgt acctggccag cgctactgcg cgcttccgct tcggccagtc ggtctcgatc
     3301 ccctcgcgat tcatcaagga ggttcccgac catctgattg agcgcaccga cttccggacc
     3361 aaccgccact acgaactcgt gtccgccggc gaccgccccg ccacggcccg cgacctcccg
     3421 ttgggacggc ttccggcaga ggaaggggtc cactatgagt acgaagagga ggaggcgctg
     3481 cgcgtcggac ggctcgtgcg ccaccctact ttcggacggg gcaaagtgct caaagtcgaa
     3541 gggcgcggcg agtcgctgat gctcgagatc catttccagg gactcggggt gaagagagtg
     3601 atggccaagt acgccaggct gaccgtcatc ggctgaggcc ggaaggcgcg ggaggcaggc
     3661 ccctggggga ggttgagggc aatctccatg gaaactatcc cgatattctc ggcggtcggc
     3721 cttctgggtg gtacaacttt caggatgccg gaaccgtcga accgaccaag gtcggtccga
     3781 attctcttgt agggggcatg cgggcggctt atattgaatt ggtggatggg attagccttt
     3841 ggcggccggc ccacgcccag ggagtctctc aacaataagg atggagatca gccggcggcc
     3901 gggaacagag ggtgcaacca cgggaacttt cgcgaccgca gccgttgttt ctaagtatag
     3961 gcaatggctt gctttaccgg tcgcggggaa gtgagatatg gtttccatga cggacggtaa
     4021 agcgaaaacc ggtgacgata agaggaagga actcgagaag cgacgggcat tcgagaaaga
     4081 ggcgctgccc cacatggacg ccctctaccg caccgctctt cggatgacca agaacaacaa
     4141 cgacgccgaa gatctggtgc aggaggcgta tgtcaaggcg tatcgcttct gggatcggtt
     4201 tgagccgggg tcgaattgtc gggcatggtt gttcaaaatc atgaccaaca ttttcatcaa
     4261 cgactaccgc tccaagtccc gctccccgat gtcggtcaat gtcgacgaaa tcgacgacag
     4321 ctttctctac ggtcagctcg cggccaccgc cccgagcgac aatcccgaga aagagttgtt
     4381 cgcgaagatt ttcgacgatg acgtgaaacg cgccatcgaa gggttgcccg aggattttcg
     4441 cctcgtcgtc gtgctgtcgt tcctcgaagg gttttcgtat caggagatcg ccgatattgc
     4501 cgatctccag ctagggacgg tgaaatcgcg cctgcaccga gggaggaaac tgcttcaaaa
     4561 agccttgtat gactacgcaa tcaagaacgg ctacatcaag gatacggcca gatgaattgt
     4621 caggaagcgc tgaacctgtt gtacgacata atcgataaag aagcctcgga aatcgacacc
     4681 agacaggtca gagaacacct cgaaagatgc agcgactgct tcgagaagta tcgccttcaa
     4741 acggaaatcc agaatttcct gaacgagaaa atccggcacg ggcaagtcga ggtcgcccac
     4801 gagcggctca aatcccgcat ccttgaccgt ctcgacgctc tggacggggc aggggaggcg
     4861 gccgatcgcg gacataccaa ggcgcggggg aacccccgcc cttttaggcg cgccgccatc
     4921 tccctgatgg cggcggctgc ggcactcatt ctcatcggcg ccggcatgct cggcgtccgg
     4981 ctctactccc accagacaaa attcgccccg ctccagcaag ctcacgtgaa cctcgccgcc
     5041 gctgtccgcg agggcggttc ttacgacgcg gcgcgcgcgg cggccggggc gcgggagaag
     5101 ttcgggtacc aaatccgccc ggccgtgggc gaattccacc tgatcgcggg gcgcagcgag
     5161 accatcatgg gcgagcagat gctgcacttt gtctacgccc tcgaccggca aaccgtgtcg
     5221 gtcttcctcg cgccggcggc ctggttcgca caggttaacg acgggcgtct cagcgaagtg
     5281 gtccgcgaca atgtccgctt ctttgaccac aactgccggg gctg
//
LOCUS       DAOPOE010000150         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_17100_length_5274_cov_4.958804, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000150 DAOPOE010000000
VERSION     DAOPOE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17100_length_5274_cov_4.958804"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKM94_13355"
     CDS             complement(<1..588)
                     /locus_tag="PKM94_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD67663.1"
                     /translation="MDRMEEELLAFLNSHGSCRKFTGREISEEQERLIAATAQRSPTS
                     SNLQAYSIVAVRDRGRKARLAELAGNQAHVAACPLFLVFCADLHRLSRLNEARGYPFH
                     GGDLELLLVATGDAALAAGRALLAAQAMGFGGVMVGGVRNHPADVCDLLGLPRLVYPV
                     MGMSLGEPAEPPKVKPRLPLAAVLFREEYSDRDFDL"
     gene            complement(672..3356)
                     /locus_tag="PKM94_13360"
     CDS             complement(672..3356)
                     /locus_tag="PKM94_13360"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOD67664.1"
                     /translation="MRARLIGVAALCLSLLAGAVTDAQAGRRVRTFGAKEAPKADAAP
                     RPDGGKAEKPFAELTKNKVAVEGLFTFYKDTTDDTWLMAIKPSQFGPIYLLGQTRSAA
                     EGAFFDNGSMGESFPFYFAKVGKTVQMMEKNLRLQADTASPSYRAVQHGISDGLWAST
                     TVKSLPNDSGTILVDPADLFVQDAQNLSYFLGQAAQMGLRFDQKNSYFTQVKSFPQNT
                     EIDVRLHYGSNKPMNGETMQNPYSMFHTIHYSLSTLPETDYVPRLADDRVGYFQTMYM
                     DYTRMDTYSPYVRYINRWNLKKKNPEARISEPVEPIVFWVENTWPVEYRDAVAEGIEF
                     WNKAYERIGFRNAVVAKQMPDTADWDPADVRYNTVRWMVMPGAGYAVGPSRANPFTGQ
                     IYDADIRVSADFIRYMFNNMEYFIKPVSFDGRFPESPDPLEEWRKYNDAYGYGCDYDR
                     ESAKEAAVGLTYLLSGAGSLAEKDSLTREYVHAYLVELVAHEVGHTLGLRHNYKGSTV
                     YSLAQLGDRSFTTVHGTTGSIMDYTPPNIGAVGQPQGEFYASVPGPYDYWVIEYGYAD
                     FGPVASPLEEKDKLAAIAGRAADPLLAYGTDEDAFGTSTKGIDPQCLTFDHGDDNVAF
                     CEHKLALTKQLWHHTVKEFERSGEGYQTVLRAFQTGWRAYSEAAGYICRYVGGIHHNR
                     YHVGDTAGVTPFTPVPAAEQRRAMKFLQDNIFAADAFTLPADLVNKLQPERESDFSGS
                     IWTTQQIDYPLHEIWLGMQNAVLSRLYDPLTLGRLRNNLARVAPAADRYTMYDMFSDV
                     RGSIWSELAQAQTINSFRRQLQLLHLQRLIQIYLSDAAAFPSDARTLAANDLDLIGTA
                     AARAAASGKLDGMSLAHCKEVVRQIDAAKGAMRQYSKM"
     gene            4169..>5274
                     /gene="arsB"
                     /locus_tag="PKM94_13365"
     CDS             4169..>5274
                     /gene="arsB"
                     /locus_tag="PKM94_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOD67665.1"
                     /translation="MIPSWLESEEQLRVAKHEPKRLSFLDRYLTLWIFLGMAAGVGLG
                     YLVPSVEGFINRFQVGTTNIPIALGLILMMYPPLAKVKYEELGDVFRNVRVLSLSLVQ
                     NWVIGPVLMFLLAVVLLRGYPEYMTGLIMIGLARCIAMVIVWNELAKGDTEYAAGLVA
                     FNSIFQVLFYSVYAWIFITVLPPLFGLKGSLVDVRMGQIAESVFIYLGIPFLAGMLTR
                     LLVLKTKGRAWYEQKFIPKISPVTLIALLFTIVVMFSLKGDLIVRIPLDVVRIAVPLL
                     IYFVVMFLVSFWLGRRIGADYSKTATLSFTAASNNFELAIAVAVAVFGINSGVAFAAV
                     IGPLVEVPVLISLVGLALHFRRRYFDEKTAGAEAP"
BASE COUNT          879 a         1681 c         1679 g         1035 t
ORIGIN      
        1 gaggtcgaag tcccggtccg aatactcctc ccgaaacagc acggccgcca acgggagccg
       61 gggtttgact ttcggcggct cggccggttc ccccagcgac atgcccatga ccgggtagac
      121 caggcggggc aggccgagca gatcgcagac gtcggccggg tggttgcgga cgccgcccac
      181 catcacgccg ccgaatccca tcgcctgcgc cgccaggagc gcccgccccg ccgccagcgc
      241 ggcgtccccc gtggccacca ggagcagctc caggtcgccc ccgtgaaacg gatacccgcg
      301 cgcctcgttc agacggctca ggcggtggag atcggcgcag aaaacgagga agagggggca
      361 ggccgcgacg tgcgcctggt tgcccgccag ttccgccagc cgcgctttcc tgccccggtc
      421 gcgcacagcg acaatcgagt aggcctgcag gttcgacgac gtcggcgaac gctgcgccgt
      481 ggcggcaatc aaccgctcct gctcctccga aatctcccgg cccgtaaatt tgcggcagga
      541 gccgtgggaa ttgagaaagg ccagcagttc ttcttccatg cgatccatag cctcctttga
      601 acaaggggcg ggaaagctgc ctttcccgcc ccgcataaac gcacggagga tgccacggcc
      661 gggggccgcg actacatttt cgaatattgg cgcatggcgc ccttggcggc gtcgatctgg
      721 cggaccactt ctttgcagtg ggccagcgac atgccgtcga gcttgccgga cgccgccgcc
      781 ctggccgcag ccgtcccgat caggtcgagg tcgttggccg ccagcgtccg cgcatccgag
      841 ggaaacgccg ccgcatcgct caggtagatc tggatcaggc gctgcaggtg cagcaactgg
      901 agctgccgcc ggaagctgtt gatcgtctgg gcctgcgcca gctcgctcca gatcgaaccg
      961 cgcacgtcgc tgaacatgtc gtacatcgtg tagcggtcgg ccgccggggc cacgcgggcg
     1021 aggttgttgc ggaggcgccc gagggtaagc gggtcataga gccggctgag gacggcgttc
     1081 tgcatgccca accagatctc gtggagcgga tagtcgatct gctgggtcgt ccagatgctc
     1141 ccgctgaagt cggactcccg ttccggctgg agcttgttca ccaggtcggc cgggagggtg
     1201 aaagcatcgg cggcgaagat gttgtcctga aggaatttca tcgcacgccg ctgttcggcc
     1261 gccggtaccg gtgtgaacgg cgtcaccccc gccgtgtcgc cgacgtggta gcggttgtgg
     1321 tgaatgccgc cgacatagcg gcagatgtac ccggccgcct cgctgtaggc acgccagccg
     1381 gtctggaacg cccgaagcac cgtctggtac ccctcgccgc tgcgctcgaa ctccttcacc
     1441 gtgtgatgcc agagctgctt ggtcagggcc agtttgtgct cgcagaacgc cacgttgtcg
     1501 tcgccgtggt cgaatgtgag gcactgaggg tcgatcccct tggtgctggt gccgaacgcg
     1561 tcctcgtcgg tcccgtaggc caggagcggg tcggcggcgc ggccggcgat cgccgcgagc
     1621 ttgtccttct cctccagcgg cgaggccacc gggccaaaat cagcgtagcc gtattcgatt
     1681 acccagtagt cgtagggacc ggggacggag gcgtagaatt ccccctgagg ctggccgacc
     1741 gcgccgatat tcggtggtgt gtagtccatg atgctcccgg tggttccgtg cacggtcgta
     1801 aaactgcggt cgccgagctg ggcgagcgag tagacggtcg agcctttgta gttgtgccgg
     1861 aggccgagcg tgtgccccac ctcatgcgcc accagttcta ccaggtaggc atggacatac
     1921 tcgcgggtga gcgaatcttt ctcggccagg ctcccggcgc ccgacagcag gtaggtcagg
     1981 ccgacggccg cctctttggc cgattcccgg tcgtagtcgc acccgtagcc gtacgcgtcg
     2041 ttgtatttcc gccactcttc cagcgggtcg ggcgattccg ggaatctccc gtcgaacgac
     2101 accggcttga tgaagtactc catgttgttg aacatgtagc ggatgaagtc ggcggagacg
     2161 cggatgtcgg cgtcgtaaat ctgcccggtg aagggattgg cgcggcttgg gccgaccgcg
     2221 tagccggccc ccggcatgac catccagcgc accgtgttgt aacgcacgtc cgccggatcc
     2281 cagtccgccg tgtcgggcat ctgtttggcc accaccgcgt tgcggaaccc gatccgctcg
     2341 taggccttgt tccagaactc gatcccctcg gccaccgcgt cccggtactc gaccggccag
     2401 gtgttctcca cccagaacac gatcggctcg accggctccg atatcctcgc ctccgggttc
     2461 ttcttcttca ggttccagcg attgatgtag cggacgtagg gactgtaagt gtccatgcgt
     2521 gtgtagtcca tgtacatcgt ctggaaatac cccacccggt catcggccag gcgcggtacg
     2581 tagtcggtct cgggcagcgt cgagagcgag tagtggatcg tgtggaacat gctgtagggg
     2641 ttctgcatcg tctcgccgtt catcggcttg ttcgaaccgt agtggagccg gacgtcgatc
     2701 tccgtattct gcgggaatga cttgacctgc gtaaaatagc tgttcttctg gtcgaaccgg
     2761 aggcccatct gggccgcctg gccgaggaag tagctgaggt tctgggcgtc ctgaacgaac
     2821 aaatcggccg gatccaccag aatcgtcccc gagtcgttcg ggagcgactt gaccgtggtc
     2881 gaggcccaca gcccgtcgga gatgccatgc tggaccgcgc gatacgacgg cgatgcggtg
     2941 tcggcctgca ggcggagatt cttctccatc atctgtaccg tcttgccgac cttggcgaaa
     3001 tagaacggga acgactcgcc catggagccg ttgtcgaaaa acgccccttc ggcggccgac
     3061 cgggtctgcc cgagaaggta gatcgggccg aactggctcg gcttgatcgc catgagccag
     3121 gtgtcgtcgg tggtgtcctt gtagaacgtg aacagcccct caaccgccac cttgttcttg
     3181 gtcaattcgg cgaagggctt ctccgccttg ccgccgtccg gccggggggc cgcgtcggct
     3241 ttcggggcct ccttggcgcc gaacgtacgc acccgccgtc cggcctgggc atcggtcacc
     3301 gcacccgcca acagcgacag gcacaaggcg gcgacgccga tcagtcttgc gcgcatgaca
     3361 actcctcgga atgtgaaaag gaatatgtgt gagacgatcg tttgcatccg gtcaaatggc
     3421 cgtatccgct tgcattatta gcactaatac ggggccgacg ccggatatgt tcccgccccc
     3481 cggccgtttt tgcccggcct aatttcctcc cgcaaccggg gtcgccgacc cgggccgaat
     3541 ctcgccggcc gccttcgcac gcccgcaccc tgacagccgg tggcgcccgc gaccacgcgt
     3601 atgattccgt ggaatctcac agggtcggaa cctcagcggc cggggctcat gcactattaa
     3661 ccgcgaatcc cacggtgggt gcggacaagg gaccgggcaa cggctttccg catcgctttg
     3721 cgttgggccg gaacgaaccc acgccgatca ggccggtgac gagcgcgatc tttaacgagc
     3781 cggaccagat gatgcgtacg cctgtaagca attaacagtc ggcgcgttag tgtcgggtcg
     3841 aggggaacgc tcctgatgac gcgggtaacc cggtttcggc cgagatgaat ctctatcgct
     3901 tccacgccgg caattccgtg cagacggaga agctacagct cacgaggcgg agagctcttc
     3961 cccgacaccg tggactcggg gcaggccggc acggtgtgcc gaacatctct ggttgtccct
     4021 ccgcgccgcc aggccgggtc tttcggaatc cggccgccca ctgattgcga agctggctcg
     4081 gcggccgctg aatttgtccg cgccacttgg gttatccggc ttgacggcgc ggattggcac
     4141 cctcatattc gcataagcgg atacaaggat gataccgtcc tggctcgaaa gtgaggagca
     4201 actgcgcgtg gcgaagcacg aacctaagcg actctcattt ctggaccgct atctcacgct
     4261 ctggatattc ctcggcatgg cggccggggt cgggctcggt tatctggtcc cctcggtcga
     4321 gggattcatc aaccggttcc aggttggcac gaccaacatt cccatagcgc tcggcctgat
     4381 actgatgatg tacccgccgc tggccaaggt gaagtatgag gagctgggcg atgtgtttcg
     4441 caacgttcgg gttctcagcc tctcgctggt gcagaactgg gtgatcggac cggtgctcat
     4501 gtttctcctt gcggtggtcc tcctgcgcgg ctacccggag tacatgaccg gcctcataat
     4561 gatcggcctc gcccggtgca tcgccatggt catcgtctgg aacgaactgg ccaaaggcga
     4621 caccgaatac gccgccggtc tggtcgcatt caactcgatc ttccaggtgc tgttctattc
     4681 ggtctacgcc tggatcttca tcacggttct gccgccgctg ttcggcctca aggggtcgct
     4741 ggtcgacgtg cgcatgggcc agatcgcgga atcggtcttc atttacctcg gcattccctt
     4801 cctggccggc atgctgaccc gccttcttgt cctcaagacc aaagggcgag cctggtacga
     4861 acagaagttc atacccaaga tcagccccgt gacgctcatc gcccttttgt tcacgatcgt
     4921 ggtgatgttc agcctgaagg gggatctcat cgtccggatt ccgcttgacg tcgtgcgcat
     4981 agcggtgccg ctgctgatct atttcgtcgt catgtttctg gtcagctttt ggctgggccg
     5041 ccggatcggc gcggactatt ccaagacggc caccctgtca tttacggcgg cgtcgaacaa
     5101 ctttgaactg gcgatcgcag tcgccgtggc ggtcttcggc atcaattccg gggtcgcctt
     5161 tgccgcggtg atcggaccgc tggtcgaggt gccggtgctc atctcgctgg tcggtctcgc
     5221 gctccatttc cgacgacggt actttgacga gaagaccgcc ggggcggagg cgcc
//
LOCUS       DAOPOE010000151         5239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_17238_length_5239_cov_5.484954, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000151 DAOPOE010000000
VERSION     DAOPOE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17238_length_5239_cov_5.484954"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PKM94_13370"
     CDS             <1..1492
                     /locus_tag="PKM94_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOD67666.1"
                     /translation="VVLGAIFFGAVHDFASLFASIRERGSSMAEIARRALGPTGFLLF
                     ILFTVVLIVLVTSAFLSLTAVSLTSLWPLDSLGLDESQTLLRVRHENGVAMGVIGGIA
                     STSVIVITLAAPVLGYLVTRKQIATWLAYLIALAVGALSVWVGFRIPVGFAPTTWMYV
                     IAVYTLFACGLPVWLILQPRDFVNVQILYAGMGAMMLGVLVMGLQGLAVSAPALNLAE
                     GSAKLGPVWPFLFITIACGAISGFHALVCGGTSSKQIAREGQVRTIGYGGMLLEGLLA
                     VLVLITIGSSLSFPEYMRVTWPEVGAGNPILAFSLSMGHLLEGAFGIPLAYGVVFGIL
                     TVEGFLITTLDVAVRLNRYLLEEFWRTVIPNPPKLLTEFWFNSAVAVVLMLALAMSNG
                     YKLIWPLFGSTNQLLAALTLIAVTVWLHRAGRSSWYTLIPAAVMICTTIASLSYYLVT
                     TYLPTGNVVLAGTDIVLLLLALGVLGLSLREFARERLAAAGRAAPS"
     gene            1558..3117
                     /locus_tag="PKM94_13375"
     CDS             1558..3117
                     /locus_tag="PKM94_13375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOD67667.1"
                     /translation="MRVFLYQNKTILPRLDAFYLATRLGVMLAVGWFGWTEHFFHTDT
                     LLAWGVLGTYVAHLAVFYGGIRGRLDLKLGYLSSIAYDLLVIPIVILYTGGLQSSFYL
                     LFFLTISVAAYMLRFWIAATVVALVSLLYLAAIRGDLTLQSLHDVVIRIGFFVVYYLA
                     LSYVSQHMRRSERRLLSLFDTLNMRTSELEKSQAQLEMIYENSRNLASILDRDGVVKE
                     VVRILGTVMGYSSYGIILRDRKDQYVYRARSVAGQVVFQPLAIDAERMPLVKKVCDLD
                     EPIRVKDIRGRHTFEPLSAGTRSVMVVPMNSHGWVNGALTVEASQADQFQERDLQMAS
                     IVARSAALALENAELHKRTEELTIIDGLTGAYNYRYFVQKLQEEKRRASRYDLPLSLI
                     MVDIDWFKRLNDTYGHEAGNRVLATLAKVIAGCIRDVDIFARYGGEEFAIILPQTTRS
                     EALVLGERIRERVEKTGMDAGNWGEVQITVSVGVSSYPENGRSQEELVSVADQALYRA
                     KGEGRNQVCVL"
     gene            3164..4162
                     /locus_tag="PKM94_13380"
     CDS             3164..4162
                     /locus_tag="PKM94_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOD67668.1"
                     /translation="MADLSKQLGQLFVIGYPGPEPPRPFMSFLATERIGGVILFEENC
                     PVHSVARDAIHRIREACGGRSPFVAIDQEGGRVSRLRGAPAEFRAAAEYGRERSVERF
                     AEEYRRSVVFMESLGINLNLAPVCDLFLDPNNACLAGRCFGRTPQEVVPFVETAVLTA
                     RGAGLLSCLKHFPGLGATAADPHRKTPVIAWDEVVWEQRERLPFAAGVAAGADMVMTT
                     HVRLRGADETIATGSSRVVEDLLRKRLGFDGPVITDDLLMAGAAELGEAGERATAAFN
                     AGHDLLLFGRDYEAAMGAYERFVDAYRRGEIANERVHSALGRVAGVKFKLDTWVQP"
     gene            4176..>5239
                     /locus_tag="PKM94_13385"
     CDS             4176..>5239
                     /locus_tag="PKM94_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOD67669.1"
                     /translation="MSLRRLISRARLTVLGLNSGTSADGLDLAVVQVGRGREKILMRG
                     GGAVRFPRPLRAAILATADSRTVEPAELVRLDNYLGQVMGRAAAGFLKTLSRRGIAVD
                     LIGSHGQTVRHVPEPVRMGGMAVRGTLQIGSPDQVAAATGCVTVGDFRQAEVALGGEG
                     APITTGAMHWLFASPREPRLIVNIGGIANYFYLPPTASGEAPTARDCGPGNSLSDALM
                     ARSFGRQFDRDGRMARRGRIDPRLLAALMESLGFARRRRSTGRELFGADLADEVVRLG
                     RRRRLRPEDLLATAGELTALGIARALHRLPASDPPPRRVYLMGGGRHNQFMVERLGEH
                     LPGWSVEAIDQLGVDGDLVEA"
BASE COUNT          787 a         1499 c         1953 g         1000 t
ORIGIN      
        1 ggtggtgctc ggggccatat tcttcggagc ggtccatgat ttcgcgtcgc tgttcgcctc
       61 cattcgcgag cggggatcgt cgatggcgga gatcgcccgg agggcgctgg ggccgaccgg
      121 atttctgctg ttcattctgt tcaccgtcgt cttgatcgtc ctggtcacct cggctttcct
      181 ctcgctgacg gcggtgtctc tgacgtcgct ctggccgctg gatagcctcg ggctcgatga
      241 gagccagacg ctgctgcggg tgcggcatga gaacggggtg gcgatgggcg tgatcggggg
      301 gattgcgtcg acctccgtga tcgtgatcac cctcgcagcg ccggtgctcg gttacctggt
      361 gacccggaag cagatcgcga cctggctggc ctacctgatc gcgctggcgg tgggggcgct
      421 ctcggtctgg gtggggttcc gcatcccggt cggtttcgcg ccgaccacgt ggatgtacgt
      481 gatcgcagtc tacacgcttt tcgcctgcgg tctgccggtg tggctgatcc tacagccgag
      541 ggatttcgtc aacgtgcaga tcctctacgc ggggatgggg gcgatgatgc tgggggtgct
      601 ggtcatggga ctgcagggtc tcgcggtgag cgcaccggcg ctcaacctcg cggaggggag
      661 cgcgaagctc gggccggtat ggccgttcct tttcatcacg atcgcctgcg gggcgatctc
      721 gggttttcac gcgctcgtct gcggcgggac ctcgtcgaaa cagatcgccc gcgagggtca
      781 ggtgcggacc atcggctacg gcgggatgct gctggaggga ttgcttgcgg tcctcgtgct
      841 catcacgatc ggctcatcgc tgtcgtttcc cgaatacatg cgggtaacgt ggcccgaggt
      901 cggggcgggc aatccgatcc tggccttctc gttgtcgatg gggcatcttc tggagggggc
      961 gttcgggatt cccttggcct acggggtggt gttcgggatt ctgacggtgg aggggttcct
     1021 gatcacgacg ctggatgtgg cggtgaggtt gaaccggtac ctgttggagg agttttggcg
     1081 cacggttatt cccaatcctc ccaagctctt aacagaattt tggttcaact cggcggtggc
     1141 cgtggtgctg atgcttgcgc ttgctatgtc caatggatac aagctgatat ggcctttgtt
     1201 cgggtcgacg aaccagcttc tggccgcttt gaccttgatt gcggtcacgg tctggttaca
     1261 tcgggccggt cggagcagtt ggtacacgct catcccggcg gctgtaatga tctgcacaac
     1321 aatagcttcg ttaagttact acctggtgac cacctacctt ccgacgggga acgtggtgct
     1381 cgcggggacc gatatcgttt tgctgctttt ggcgctgggc gtgctggggc tgtcgcttcg
     1441 cgaattcgca cgggagcgat tggccgccgc cggccgcgcg gcgccgtcat aagctgttcg
     1501 ggcgcattaa cgttcatccg attcagccga tataacggaa gaagcggatg gaaataaatg
     1561 cgcgtattcc tctaccagaa caaaaccatt ctgccgcggc tggatgcttt ctacctggcc
     1621 acccgactgg gcgtgatgct ggcggtcggc tggttcgggt ggaccgagca tttcttccac
     1681 accgacacgc tgctggcgtg gggggtcctc ggcacatatg tcgcgcacct ggcggtgttc
     1741 tacgggggaa tccggggacg gctggatctg aagctcggct acctctcgtc gatcgcttat
     1801 gatctcctcg tcattccgat cgtcatcctg tacaccggcg ggctgcagag cagcttctat
     1861 cttctgtttt tcctgacgat ttcggtagcc gcgtacatgc ttcggttctg gatcgccgcc
     1921 acggtggtcg ccctggtgag ccttctgtat ctggcggcga tccgggggga tctcacgctg
     1981 cagtcgctcc acgacgtggt gatccggatc gggttcttcg tcgtgtacta cctggcgctc
     2041 tcctacgtgt cgcagcacat gcggcgttcg gagaggcgcc tgctcagcct gttcgacacc
     2101 ctcaacatgc ggacgtcgga gctggagaag tcgcaggcgc agctggagat gatctacgag
     2161 aactcccgca acctggcctc gattctcgac cgggacgggg tggtgaaaga ggtggtgcgg
     2221 atcctcggca cggtcatggg ttattccagc tacggcatca tcctgcggga ccgcaaggac
     2281 cagtacgtct accgggcgcg gtcggtggcc gggcaggtgg tattccagcc gctggcgatc
     2341 gacgcggagc gcatgccgct ggtcaagaaa gtgtgcgatc tcgacgagcc gatccgggtg
     2401 aaggatatcc ggggtcggca cacgttcgag ccgctgtcgg ccggcacgcg gtcggtgatg
     2461 gtggtgccga tgaattcgca cgggtgggtg aacggggcgc tcacggtgga ggcgtcgcag
     2521 gccgaccagt tccaggagcg ggacctccag atggcgtcga tcgtggcgcg ctcggcggcg
     2581 ctggcgctgg agaacgctga gctgcacaag cggactgagg agctgacgat cattgacggg
     2641 ctgacgggcg cgtacaacta ccggtacttc gtgcagaagc tgcaggagga gaagcggcgg
     2701 gcgagccggt acgatctgcc gctgtcgctg atcatggtcg atatcgactg gttcaagcgg
     2761 ctgaacgaca cctacgggca cgaggcgggg aaccgggtgc tggcgacgct ggcgaaagtc
     2821 attgcggggt gcatccgcga tgtcgatatt ttcgcacggt acggcggcga ggagttcgcc
     2881 atcattctgc cgcaaaccac gcggagcgag gcgctggtgc tcggggagcg catccgggaa
     2941 cgggtcgaga agaccgggat ggatgccggc aactggggag aggtgcagat caccgtctcg
     3001 gtcggggtga gttcgtatcc cgagaacggg cggtcgcagg aagaattggt gtcggtagcc
     3061 gaccaggcgc tctaccgggc gaaaggggaa gggcgcaacc aggtgtgcgt gttgtgagag
     3121 gaaacggaaa caaggaacca atgaccgata gcagggatga gccatggcgg atttgtcaaa
     3181 gcagttgggc cagttgttcg tgatcggata ccccgggccg gagccgcccc gcccgttcat
     3241 gagctttctc gcgacagagc ggatcggggg ggtgatcctg ttcgaggaga actgtcccgt
     3301 gcacagcgtg gcgcgcgatg cgattcaccg catccgcgag gcctgcggcg gaaggtcgcc
     3361 gttcgtggcg atcgaccagg aaggcggccg ggtcagccgc ctccgcggcg cgccggcgga
     3421 gttccgggcc gccgccgagt acggccgcga gaggtcggtg gagcggttcg ccgaagagta
     3481 ccgccgatcg gtggtgttca tggaatcgct cgggatcaat ctcaacctcg ccccggtgtg
     3541 cgatctcttc cttgatccga acaacgcgtg cctggccggg cgctgcttcg ggcggacgcc
     3601 ccaggaggtg gtgccgtttg tcgagacggc ggtgctgacg gcccgggggg cgggtctgct
     3661 cagttgtctg aaacacttcc ccggcctcgg ggcgacggcc gcggatccgc accgcaagac
     3721 gccggtgatc gcgtgggacg aggtcgtgtg ggagcagcgg gaacgtctcc ccttcgccgc
     3781 cggcgtggcc gcgggggcgg acatggtcat gaccacccat gtgcggctga gaggggccga
     3841 tgagacgatc gcgacggggt cgtcgcgggt ggtggaggat ctgctgcgga agcggctggg
     3901 gttcgacgga ccggtgatca cggacgacct gctgatggcc ggggcggcgg agctcggcga
     3961 ggcgggcgag cgggcgacag cggccttcaa cgccggccac gatctgctcc tgttcgggcg
     4021 ggactacgag gcggccatgg gggcctacga gcggtttgtc gacgcctacc ggcgcgggga
     4081 gatcgcgaat gagcgggtgc actcggcgct cggccgggtt gccggcgtga agttcaaact
     4141 ggacacctgg gtgcagccgt gactttcttg aagggatgtc gctgcggcgc ctcatatcgc
     4201 gagcgcggct gaccgtgctc ggattgaact cgggcacctc ggccgacgga ctggatctgg
     4261 cggtggtgca ggtgggccgc ggccgggaga agatcctcat gcggggcggc ggggccgtgc
     4321 ggttcccgcg gccgctgcgg gccgcgatcc tggcgaccgc cgacagccgc acggtggaac
     4381 cggccgagct ggttcgcttg gacaactacc tgggacaggt catggggaga gcggccgccg
     4441 ggtttctgaa gacgctttcg cgccggggga tcgcggtcga tctgatcggt tcgcacgggc
     4501 agacggtgcg gcatgtcccg gagccggtgc gcatgggggg aatggcggtg cgcgggacgc
     4561 tgcagatcgg atcgcccgac caggtggcgg cggcgaccgg gtgcgtgacg gtgggggact
     4621 tccgccaggc ggaggtcgcg ctcggcggcg aaggggcgcc gatcaccacg ggggcgatgc
     4681 actggctctt cgcatcgccg cgcgagccac gcctgatcgt gaacatcggg gggatagcca
     4741 attatttcta tctcccgccg acggcgtcgg gcgaggcgcc cacggcgcgc gactgcggtc
     4801 cgggcaacag cctctccgat gcgctcatgg cgcggtcgtt cgggcggcag ttcgaccggg
     4861 acgggcgcat ggccaggcgg gggcggatcg acccgcgcct gctggcggcg ctgatggaaa
     4921 gtctggggtt tgcacggcgc cgccggtcga ccgggagaga gctcttcggg gcggatctcg
     4981 ccgatgaggt ggtccggctg gggcggcggc gccggcttcg gccggaggat ctgctggcca
     5041 cggcggggga gttgacggcc ctcgggatcg cccgcgccct gcaccgcctt cccgcctccg
     5101 atccgccgcc gcggcgggtg tatctcatgg gcgggggacg gcacaaccag ttcatggtcg
     5161 aacgcctggg ggagcatctc cccgggtggt cagttgaggc gatagatcag ttgggagtgg
     5221 acggcgactt ggtggaggc
//
LOCUS       DAOPOE010000152         5197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_17420_length_5197_cov_4.997277, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000152 DAOPOE010000000
VERSION     DAOPOE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17420_length_5197_cov_4.997277"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1616
                     /locus_tag="PKM94_13390"
     CDS             105..1616
                     /locus_tag="PKM94_13390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD67670.1"
                     /translation="MSYALRLKSGLLAALCALVVFGCGGAEQPAGEADAHAGHAHGPG
                     GHAEAAPIEPPAAQADDWCAEHRVPESQCTACDPALIAAFIAAGDWCEEHGLPESHCR
                     LCNPGLGFPQEEPASSAADIAAEEIRVSLFFRPNALVCATNDALIQLATARTAERAGL
                     TVQQVRAAELETTIEAPAEVVFDETRTTAVATTVAALVARWVAAPGETVREDQVLAIL
                     QSPEVAALESQLLSAHADLLVHTNELARQEELRGRDLISDAELERQRALFAQAKAAYV
                     AGRGLLRAAGLSEDDLDEIIAEGRISSTFALRAPTAGLLVERRALPGELQEAGSALAR
                     LADPAVMWIEARLTEEQLRQVAVGEPLTFASDGRGLSRVGGKIIWVARFLDPHSRTGT
                     VRAQVVDHRHTLQAGEFGRVTIARTGHRRVALVPRDAVQWEGCCNVVFVREAADRYRP
                     RKVEFTDGAGPYYQVLRGLEPGEEVVVDGAFLLKTELKKASIGAGCCGLDPVG"
     gene            1625..4693
                     /locus_tag="PKM94_13395"
     CDS             1625..4693
                     /locus_tag="PKM94_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOD67671.1"
                     /translation="MLGYIVDFMLRRRYAVLAATAVLCLFGLAALWHLPFDAFPDTTP
                     VLVQVNVAAPGWAPEDLERLVAYPVEQALTGLTGLAEVRSVTRYGLCQVTTIFDDGVD
                     LYLARQQVSERLPGIALPDGIGAPELGPVSTGLGEIFHYIVVADSGDVTDPRTVQDWL
                     IKPQLLAVPGTAEINSWGGFVKEYLILFDPTRLARYNLPLDDMAAAIREQLGNVPGGQ
                     IIRGGEMTLVRGIGQVENVGQIENIVVAVVEGTPIAVRDVAEVVIGHELRRGATTYNG
                     RGEAVLGLGFMVTGENPAEVTERLARRLGEAAGGLPAGVRAVPVYERTKMVGEVLSTV
                     EHNLFYGAVLVVAVLLAFLGNLRAGLIVASAIPLAMLFAFDLMSRAGITGSLMSLGAI
                     DFGLAVDNGVIQVENSIRRLSQAGGSASRLHVIRDAILEVRKPTLFGEIIIIIVYLPI
                     LTLEGVEGKLFRPMALTVVFMLTGSLILSFTVLPALIGTLLHKGVRAREPIFVGWLRR
                     LYAPALEAALRYRRAVLLAAAVLVAGGVLLFLRLGAEFVPRLSEGTVVINLVRLAGIS
                     VDEAVAYNSRVEKNLLAAFPDEIDHIWTRTGTAELSTDPMGLEQSDMFISLTPRRQWT
                     KASTQEELVAAIDAALADLPGQNRIFTQPIEMRVNEMVAGIRGDIGVKLFGDDLAVLE
                     EKAEEIAALLGAIPGAADVTIEQLTGQPQLRLAVDRQRLARFGLSARDVLTAIESLGG
                     LRVGDVYEGQQRFALTVRVDTTLVSGPEDIGALLVQSAAGSLVGLDRVTTATLDEGPA
                     VITREWSKRRIVIQCNARGRDLGSLVREIRERLASDITLPTGYFLRLGGQFEHLERAR
                     VRLAIVVPAALLLIFGLLYWTYRSTRDALLIFSGVPLAALGGAAMLAVRGMPFSISAG
                     VGFIALSGIAVLNGLVLVSTIKHLRAEGREITEAVRESALVRLRPVFMTALVAALGFV
                     PMAISTGVGAEVQRPLATVVVGGILTSTALTLIVLPALYLTFGQKTESEV"
     gene            complement(4712..>5197)
                     /locus_tag="PKM94_13400"
     CDS             complement(4712..>5197)
                     /locus_tag="PKM94_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOD67672.1"
                     /translation="TTVQLDGPPCHCLNRGCLEALAGGWAIARRARELALASPRDGAL
                     LLSLVDGDPERLSAQAAAEAFRRGDPLAQRIIGEAVAALVGGCTSFVNAFNPRRLILG
                     GGVIEGCPELIGLVAEGVRQRALPAAAGDLEIVKAHLGADAGPVGAAHLARDADSAAH
                     R"
BASE COUNT          755 a         1728 c         1883 g          831 t
ORIGIN      
        1 cggcggcgtc cgcgcgtcct tgtcttcccc aaaacgaatt gccgaaccca ggtgcggccg
       61 gcggcgcggc ctcacacctg aacgagaaat ggagatctgc acacatgagt tacgcgttga
      121 gactgaagtc agggctcttg gcggcgctgt gcgccctggt ggtgttcgga tgcggcggcg
      181 cagaacagcc ggccggggag gccgatgccc acgcggggca cgctcacgga ccgggcgggc
      241 acgcggaggc ggcccccatc gagccgccgg cggcgcaggc cgacgactgg tgcgccgaac
      301 accgggtgcc ggaatcgcag tgcacggctt gcgaccccgc cctgatcgcc gcgttcatag
      361 cggccggcga ctggtgcgag gagcacggtc tccccgagtc gcactgccgg ctctgcaacc
      421 cggggctggg ctttccccag gaggagccgg cgagttcggc ggcggatatc gccgccgagg
      481 aaatccgggt ctccctcttc ttccgcccca atgccctggt ctgcgcgacc aatgacgcgc
      541 tgatccagct tgccacagcc cggacggcgg agcgcgccgg actgacggtg cagcaggtgc
      601 gggcggctga actcgagacg acgatcgagg cgccggcgga ggtcgtgttc gacgagacca
      661 ggacgaccgc ggtcgccacc accgtggcgg cgctcgtcgc gcgctgggtg gccgcgccgg
      721 gggaaacggt gcgcgaggac caggtgctcg cgatcctgca gtcgccggag gtggccgcgc
      781 tggaatcgca actgctctcc gcccacgccg atcttctcgt gcacaccaac gagctggccc
      841 ggcaggagga gctgcgcggc cgcgacctga tcagcgacgc cgagctggaa cgccagcggg
      901 cgctctttgc gcaggccaag gcggcctatg tcgccggccg ggggctgctc cgggccgccg
      961 gcttgagcga ggatgacctc gatgagatca tcgccgaggg ccgcatttcc agcacctttg
     1021 cgctgcgcgc gccgaccgcc ggactcctcg tcgagcgccg ggcgctgccg ggagaacttc
     1081 aggaggcagg cagcgccttg gcccgactgg ccgatcccgc ggtgatgtgg atcgaggccc
     1141 gcctcaccga ggaacaactt cgccaggtgg cggtcgggga gccgctgacg tttgcctcgg
     1201 atggccgcgg gttgagccgg gtcggcggga aaatcatctg ggtcgcgcgg tttctcgacc
     1261 cccactcgcg caccgggacg gtgcgggcgc aggtggtcga tcaccggcat acgctccagg
     1321 ccggggagtt cgggcgtgtc acgattgccc ggaccggcca ccgccgggtc gcgctcgtgc
     1381 cgcgcgacgc cgtgcagtgg gagggctgtt gcaacgtggt gttcgtgaga gaagcggccg
     1441 accgctaccg cccgcgcaaa gtcgagttca ccgacggcgc cggtccgtac taccaggtgc
     1501 tgcgcggact cgaacccggc gaagaggtgg tagtcgatgg cgccttcctc ctgaagaccg
     1561 aactgaaaaa ggccagcatc ggcgctggct gctgcggcct cgacccggtc ggctgagggg
     1621 gagtatgctg ggatacatag tcgacttcat gctgcgccgg cgatacgccg tgctcgcggc
     1681 caccgccgtc ctctgcctgt tcggcctggc ggccctctgg catctcccct tcgacgcttt
     1741 cccggacacc acgccggtcc tggtgcaggt gaacgtcgcc gccccgggct gggcgccgga
     1801 ggatcttgaa cggctcgtgg catacccggt cgagcaggcg ctcacggggc tgaccgggct
     1861 ggcggaagtg cgctcggtca cccgctacgg gctgtgccag gtcactacga ttttcgacga
     1921 cggcgtcgat ctctacctgg cccgccagca ggtgagcgaa cggctgcccg gcatcgccct
     1981 gcccgacggg atcggggcgc ccgaactggg gccggtctcc accggcctgg gggaaatctt
     2041 ccactacatc gtggttgccg actccgggga cgtcaccgac ccccgcacgg tgcaggactg
     2101 gctgatcaag ccgcagttgc tggcggtgcc ggggacagca gaaatcaaca gctggggcgg
     2161 gttcgtcaag gagtatctca ttctctttga cccgacccgc ctcgcgcggt acaacctgcc
     2221 gctcgacgat atggcggcgg cgattcgcga gcagctcggc aacgtgcccg gcgggcagat
     2281 catccgggga ggcgagatga ctctcgtgcg ggggatcggc caggtggaaa atgtcggcca
     2341 gatcgagaac atcgtggtcg ccgtggtcga gggaacgccg atcgcggtgc gcgatgtcgc
     2401 cgaggtcgtg atcgggcacg aactgcgccg gggcgcgacg acctacaacg ggcgcggcga
     2461 ggcggtgctt ggactcgggt tcatggtcac cggcgagaat ccggccgagg tcaccgaacg
     2521 gctggcccgc cggctggggg aagcggccgg ggggctgccg gcgggcgtgc gggcggtccc
     2581 ggtctatgag cgcacgaaaa tggttggcga agtgctctcg actgtagagc ataatctctt
     2641 ctacggcgcc gtgctggtgg tggcggtgct gctggcgttt ctcggcaatc tccgcgcggg
     2701 cctcatcgtc gcctccgcca tcccgctggc gatgctgttc gcgtttgatc tcatgtcgcg
     2761 cgcggggatc acgggaagcc tgatgagcct cggggccatc gacttcggcc tcgccgtcga
     2821 caacggcgtg atccaggtcg agaactcgat ccggaggctg tcgcaggccg gcggttcagc
     2881 ctcgcgcctg cacgtgatcc gcgacgccat cctcgaggtg cgcaagccga cgctcttcgg
     2941 cgagatcatc atcatcatcg tctacctccc cattctcacc ctcgaggggg tcgaggggaa
     3001 gttgttccgg ccgatggcgc tgacggtagt gttcatgctg accggctcgc tcatcctgtc
     3061 gttcacggtc ctcccggcgc tcatcggaac gctgctgcac aaaggggtcc gcgcgcgcga
     3121 gccgatcttc gtcggctggc tgcggcggct ctatgcgccg gcgctcgagg cggccctgcg
     3181 gtaccgccgg gccgtgctgc tggcggcggc cgtgctggtc gccggagggg tgttgctctt
     3241 tctccggctc ggcgccgagt ttgtcccgcg cctgtcggag ggaacggtcg taatcaacct
     3301 cgtgcggctg gccgggattt cggtcgacga agcggtggct tacaactcgc gcgtggagaa
     3361 gaacctgctg gccgctttcc ctgatgagat cgaccacatc tggacgcgca ctggcacggc
     3421 ggaactctcg accgatccga tggggctgga gcagtcggat atgtttattt ccctcacacc
     3481 gcggcggcag tggacgaaag cgtcgaccca ggaggaactg gtggcggcga tcgatgccgc
     3541 gctggccgac ctgccgggtc agaaccgtat cttcacccag ccgatcgaaa tgcgggtgaa
     3601 cgagatggtg gccgggatcc gcggcgacat cggcgtcaag ctgttcggcg acgatctggc
     3661 ggtgctggaa gagaaggccg aggaaatcgc cgcgctgctc ggagcgattc ccggcgcggc
     3721 cgacgtgacc atcgagcaac tcaccggcca gccgcagctg cgcctcgcgg tcgaccgcca
     3781 gcggctcgcc cgcttcgggc tgtcggcccg cgatgtcctc accgcgatcg agagcctcgg
     3841 cgggctccgc gtgggcgacg tctacgaggg acagcagcgc tttgcgctta cggtgcgggt
     3901 ggacacgacg ctggtcagcg ggccggagga tatcggggcg ttgctggtgc agtcggccgc
     3961 cgggagcctg gtgggactgg accgggtgac gacggcgaca ctcgacgagg gcccggcggt
     4021 gatcacacgc gaatggagca agcggcggat cgtaatccag tgcaatgccc gcggccgcga
     4081 cctggggtcg ctggtgcgcg agatccgcga gcggctggcc tcggacatca cactgccgac
     4141 cggctatttc ctccgccttg gcggccagtt cgagcatctc gaacgggccc gggtgcggct
     4201 cgcgatagtt gtcccggcgg cgctcctgct catctttggc ctgctctact ggacctaccg
     4261 atcgacgcgc gacgccctct tgatcttctc cggcgtgccg ctggcggccc tggggggcgc
     4321 ggccatgctg gccgtgcggg ggatgccgtt ttcgatatcg gccggggtcg gatttatcgc
     4381 gctctcgggg attgccgtgc tcaacggact ggtgctcgtg tcgaccatca agcacctccg
     4441 tgcggagggg cgcgagatca ccgaggcggt gcgcgagagc gccctggtgc ggctgcggcc
     4501 ggtgttcatg accgcactcg tggcggcgct cggattcgtc ccgatggcga tttccaccgg
     4561 cgtgggggcc gaggttcagc ggccgctggc aaccgtcgtg gtcggcggca tcctcacctc
     4621 gaccgcgctc accctgattg tgctgcccgc cctctacctg actttcggcc agaagacgga
     4681 atcggaggtc tgaggattgg ggcgcgccgg ctcaccggtg cgccgcactg tcggcatccc
     4741 gcgccagatg ggcggcgccg accggccccg cgtcggcgcc gagatgcgcc ttgacgattt
     4801 cgagatcgcc ggcggcggcc ggcagggccc gctgccgaac gccctcggcg accaggccga
     4861 tcaactcggg gcagccctcg atgactccgc cccccaggat gagccggcgc ggattgaaag
     4921 cgttcacgaa cgaggtgcac ccgccgacca gggccgcgac cgcttcgccg atgatccgtt
     4981 gggcgagcgg atcgccgcgg cggaaagcct cggccgccgc ctgggccgag aggcgctccg
     5041 ggtcgccatc gaccagactg aggagcaggg cgccgtcgcg cggcgaagcc agcgccagct
     5101 cccgggcgcg ccgggcgatc gcccagccac ccgccagcgc ttcaaggcac ccgcggttca
     5161 ggcagtggca tggcgggccg tcgagttgaa ccgtcgt
//
LOCUS       DAOPOE010000153         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_17660_length_5138_cov_4.302971, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000153 DAOPOE010000000
VERSION     DAOPOE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17660_length_5138_cov_4.302971"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKM94_13405"
     CDS             complement(<1..736)
                     /locus_tag="PKM94_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67673.1"
                     /translation="MRRMTIADGRLPALFVACALGVSGLVMDGCGGKAAISRPPNFTE
                     SSRSATGDSGSKAAPASSGAVAAAAPADQDTSLATNHDPAGKRSREELVREYNRRNAT
                     AISDPSPSTDTLVEMGDVGDSTAAVDDELWRQLDLAEEYHAMGVIANREGSWEEAQYY
                     FEQGLTILASLDIEGDSLETPEAAAYSILLDDVIADYRVTLLSLGDLQGDVSPAVLIE
                     RFGDLANNLSNDSMLVFTTEPEEITFD"
     gene            complement(915..1844)
                     /gene="miaA"
                     /locus_tag="PKM94_13410"
     CDS             complement(915..1844)
                     /gene="miaA"
                     /locus_tag="PKM94_13410"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD67674.1"
                     /translation="MPEPRAIPILCGPTGAGKTAAAVEAAHGRPIEVVSADSRQMIRG
                     FDIGTAKPTPEERAAVPFHLVDVIDPGERYTAFQFIDDATAAIDDILRRGAIPLVVGG
                     TGFYLRALTTGIVEIAEGDLGIRERLEREMAEQGAEALYAHLTRIDPLEAARLHPNNR
                     VRVIRALEIFEVTGRTRSELAVTGRYRKAAHRFAYLCLAPERPELYARIDARVEQMMA
                     AGLLAEVRGLAARIGPEQVRKANVIGYTELLDHAEGRLALDEAVALVKQNHRRYAKRQ
                     LTWFRHQADCRFFADRPSLMQELRANLAALQGA"
     gene            complement(1837..3711)
                     /gene="mutL"
                     /locus_tag="PKM94_13415"
     CDS             complement(1837..3711)
                     /gene="mutL"
                     /locus_tag="PKM94_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOD67675.1"
                     /translation="MTRPTDSPAPQRIRPLPERVVNKIAAGEVIERPAAVVKELVENA
                     LDAGADRVDIVVEKSGARLIKIVDNGCGIAEDQVEIAFARHATSKIAVFDDLNELYTY
                     GFRGEALPSVASVSRLRMVTRPREAAHGTEIIYEGGVLMTKRPSAAAPGTTIEVENLF
                     FNTPARRKFLKAETTEARHISRMAMALAIGRWDVAFSYTLNERKMFAAPAAEAVAERV
                     PTLLSPGRPFLPVAGEAGPVRLSGCIGRPDMIQNNRYGQFFFINRRFIQSATLAHAFL
                     AGYGELIPKGMFPVGALLIEVDPREVDVNVHPTKAEVRLSNERELHEAVRRLVSETLR
                     QDGIVPTFPLRTGDGEKSGGGPPGIRPVPVPPSRQGVIPGVYHARPVSLDQLGEFYRP
                     PEDGPAAADPALPPGLTVDTVTGEVRGDQPADSGPAAPGPSQGFRFIGRFANLYLLIE
                     SAEELYVVDQHTAHERVLYEETLRRLAEQSLSGQSLLFPAQVELSPEQFAVFEESCDL
                     LNRSGFAVAPFGGRTVTIDATPSVLARRQRSPDTMFLHILDDLGSLRRAGQDLTKAMA
                     QSIACRAAVMAGDRLSEHEAVHLLEALLQCRDRYSCPHGRPTFVRITRSDLDRQFGRA
                     "
     gene            complement(3708..>5138)
                     /gene="mutS"
                     /locus_tag="PKM94_13420"
     CDS             complement(3708..>5138)
                     /gene="mutS"
                     /locus_tag="PKM94_13420"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOD67676.1"
                     /translation="AAAHSPLLTELAAALPDTTEIVDRLAGALVDEPPLTHLKGDIFR
                     RGYAPRLDALNDGIRDARQYIAGLAKTERERTGIGNLKVGFNKVFGYYIEITKSHLGL
                     VPPDYIRKQTLVNAERFITPELKEKEDLIVSAEEKIFKLEEELYLELMEFLNTRIAAI
                     KLAAELAAELDLVSGLAETAVERGYVQPEIYEDARLRITGGRHPVIEAVLPPGSFVAN
                     DVTLSPDAGCIHILTGPNMSGKSTYLRQIGLIVILAQIGSFVPAEKAEIGLADRVFTR
                     VGAVDNLARGQSTFLVEMVETANILHNATARSLVLLDEVGRGTSTFDGLSVAWAVVEH
                     INEHIRARTVFATHYHELTGMAEIYESVHNYQVQVKKWEDRVVFLHHIIPGGCDDSYG
                     IEVARLAGLPRSAIARARQILRLLESGKFTRSELGRGLYKERVQPTLFDQKPSATEEQ
                     IRALDLDRMTPLEALDALRKMKEDLA"
BASE COUNT          807 a         1717 c         1707 g          907 t
ORIGIN      
        1 tatcgaaagt gatctcctcc ggctcggtcg tgaacaccag catggagtcg ttgctgagat
       61 tgttcgcaag atcgccgaac cgctcgatca gcacggcggg cgagacgtcc ccctgcagat
      121 cgccgagcga cagaagggtg acccggtagt cggcgatgac gtcgtccagg aggatgctgt
      181 aggccgccgc ctcgggcgtc tccagcgagt cgccctcgat gtcgagcgac gccaggatgg
      241 tcaggccctg ctcgaaatag tactgggcct cctcccagct tccctcgcgg ttggcgatca
      301 cgcccatcgc gtggtactct tcggccaggt cgagctgccg ccacagctcg tcatccacgg
      361 ccgctgtcga atcccccacg tcccccattt ccacgagcgt gtcggtcgag ggcgaggggt
      421 cgctgatcgc ggtcgcattg cggcggttgt attcgcggac caattcctcg cgggagcgtt
      481 tgcccgcggg atcatgattt gtcgcaaggg aagtgtcctg gtcggccggt gcggccgcgg
      541 ccacggcgcc ggaggatgcc ggcgcggcct tcgaaccgga gtcgccggtg gcgctccgcg
      601 aggattcggt gaaattcggc ggtcgggaaa tggcggcctt gccgccgcac ccgtccatga
      661 ccagccccga taccccgagt gcacatgcca caaacagggc cggcagacgg ccgtcggcaa
      721 tcgtcattcg tctcatgcgt gatcccacgg ttgcggtttt tgatcccatt atcggtcatc
      781 cgcccacccc cctttaaagg ggcggtcatt gtgtcaacct ggccaaagta cgggtgtaaa
      841 aacctcgtgt caattttcaa caagccggcc cggtgatttt ctcgtactgc gcaaaaagtg
      901 cacaggcccc gccgtcacgc gccctgcagc gccgccaaat tggcgcggag ctcctgcata
      961 agtgacggtc gatcggcgaa aaatcggcaa tcagcctggt ggcggaacca tgtcaactgg
     1021 cgtttggcgt agcggcggtg gttctgtttg accagggcca ccgcctcgtc cagcgccagg
     1081 cgcccctccg cgtgatcgag cagctcggtg tagccgatca cgttggcctt ccgcacctgt
     1141 tccggaccga tccgggcggc caggccgcgc acttccgcga gcagccccgc cgccatcatc
     1201 tgctccaccc gcgcatcgat gcgggcgtac agttccgggc gctccggggc caggcagagg
     1261 tatgcaaagc gatgcgcggc cttgcggtat cggccggtca cggccagttc cgagcgtgtg
     1321 cggccggtca cttcgaatat ctccagcgcc cggatcacac gcacgcggtt gttcgggtgg
     1381 agccgggccg cctccagcgg atcgatccgc gtgaggtggg cgtagagtgc ctccgcgccc
     1441 tgctccgcca tctcccgctc cagtcgctcc cggatcccca gatccccctc ggcgatctcc
     1501 acaattccgg tcgtgagcgc ccgcagatag aatcccgtac ccccgaccac caggggtatg
     1561 gctccgcggc ggagaatgtc gtcgatcgcc gccgtcgcat catcgataaa ctgaaaagcg
     1621 gtgtaccgtt ctccgggatc gataacatcg acgaggtgga agggcacagc cgcgcgctcc
     1681 tcgggcgtcg gtttggccgt cccgatatcg aatccccgga tcatctggcg ggaatcggcg
     1741 gagacgactt cgatgggccg cccgtgcgcg gcttcgacgg ccgccgccgt cttgccggcg
     1801 ccggtcggcc cgcagaggat cggtatggcg cgcggctcag gcacggccga attgcctgtc
     1861 gaggtcgctg cgcgtaatgc gcacgaaagt cgggcgcccg tgcgggcagg agtagcggtc
     1921 ccggcactgc aggagggcct cgaggaggtg gaccgcctcg tgctcgctca ggcggtcgcc
     1981 cgccatcacc gccgcccggc aggcgatcga ttgcgccatc gccttggtga gatcctgccc
     2041 ggcccggcgc agtgatccga ggtcatcgag aatgtgcagg aacatcgtgt cgggcgacct
     2101 ctgccggcgg gcgagcaccg agggcgtcgc gtcgatcgtc accgtccgcc cgccgaaggg
     2161 cgccacggcg aatcccgacc ggttgagcag atcgcatgat tcctcaaaca cggcgaattg
     2221 ctcggggctc agctccacct gggccggaaa caggaggctc tgcccgctca gcgactgctc
     2281 ggccagccgc cgcagagtct cctcgtagag cactcgctcg tgcgcggtgt gctggtccac
     2341 gacatagagc tcctcggcgc tttcgatcag caggtagaga ttggcgaacc ggccgatgaa
     2401 ccggaaaccc tgagagggac ccggagccgc cgggccggag tccgccggct gatcgccgcg
     2461 cacctcgccg gtcaccgtat ccaccgtcag ccccggcggc agcgccggat ccgccgcggc
     2521 cgggccgtct tccggcgggc ggtagaactc tccgagttgg tcgaggctga ccggccgcgc
     2581 gtggtagacg ccggggatta cgccctggcg ggagggaggc acgggcacgg gacggatacc
     2641 cggcggcccg ccgccgcttt tctccccgtc gcccgtacgc aacgggaaag tcgggacgat
     2701 cccgtcctgc cgcagcgtct cgctgaccag gcggcggacg gcctcgtgga gttcccgctc
     2761 gttcgacagc cgcacttccg ccttggtcgg gtgcacgttg acgtcgacct cgcgcgggtc
     2821 gacttcgatg aggagcgcgc cgacagggaa catgcctttg ggaatcaact cgccgtagcc
     2881 agccaggaat gcgtgcgcca gcgtcgccga ttgaatgaac cggcgattga tgaagaaaaa
     2941 ctgcccgtag cggttgttct gaatcatgtc ggggcgcccg atgcagccgc tcaggcgcac
     3001 cggcccggct tccccggcaa ccggcagaaa tggccggccc ggactcaaga gggtcgggac
     3061 gcgttcggcc acggcctcgg ccgccggcgc cgcgaacatc ttccgctcgt tgagcgtgta
     3121 cgagaacgcg acatcccagc gcccgatcgc cagcgccatg gccatacgcg agatatgccg
     3181 ggcctcggtc gtttcggctt tgaggaattt ccgccgcgcc ggggtattga aaaacaggtt
     3241 ctcgacctca atcgtggtgc ccggcgccgc cgcagacggc cgcttggtca tcagcacccc
     3301 cccttcgtag atgatctcgg ttccgtgcgc cgcctcgcgc gggcgcgtca ccatgcgcag
     3361 ccgcgagacg gaggccaccg agggcagcgc ctcgccgcga aatccgtagg tgtacagctc
     3421 gttgagatcg tcaaacacgg cgatcttcga ggtcgcgtgg cgcgcgaatg cgatttcgac
     3481 ctggtcttcc gcaatgccgc agccgttgtc gacaatcttg atcagccggg ccccggattt
     3541 ttcgaccacg atgtcgaccc ggtcggcgcc cgcgtcgagc gcattttcga ccagttcctt
     3601 gaccacggca gccggccgct cgatcacttc gcccgccgca atcttgttga ccacccgttc
     3661 gggcaggggg cgtatccgct gcggcgccgg ggaatcggtc ggccgggtca tgcgagatcc
     3721 tctttcattt tgcgcagcgc gtcgagcgcc tccagcggcg tcatgcggtc aaggtcgagg
     3781 gcccggattt gttcttcggt tgccgacggc ttctgatcga agagcgtcgg ctgcacgcgc
     3841 tccttataca gccctcgtcc cagctcgctg cgggtgaatt ttcccgattc gagcagacgg
     3901 agaatctggc gggcgcgggc gatcgccgac cggggcagcc cggccagccg ggccacctcg
     3961 atcccgtagg agtcatcgca cccgccgggg ataatgtggt ggaggaatac cacccggtct
     4021 tcccacttct tcacctgcac ctggtagttg tggacggact cgtagatttc cgccatcccg
     4081 gtcagttcgt ggtagtgggt ggcgaacacg gtgcgcgcgc ggatgtgctc gttgatgtgc
     4141 tcgacgaccg cccaggcgac cgagagtccg tcgaacgtcg acgtcccccg ccccacttcg
     4201 tcgagcagca cgagcgaacg cgcggtggcg ttgtggagga tgttcgccgt ctccaccatc
     4261 tcgaccagga aggtcgactg cccgcgggcg aggttgtcga cggcgcccac ccgcgtgaac
     4321 acccggtcgg ccagcccgat ctccgctttc tccgccggca cgaacgaccc gatttgggcg
     4381 aggatcacga tgagtccgat ctggcgaagg taggttgatt tgcccgacat gttcggcccg
     4441 gtgaggatgt ggatgcaccc cgcgtcgggc gagagcgtca cgtcgttggc gacgaacgag
     4501 ccgggcggca gcacggcctc gatcaccggg tggcggccgc ctgtgatccg cagccgggcg
     4561 tcctcgtaaa tctccggctg cacgtacccc cgctccaccg ccgtctcggc cagcccggag
     4621 acgagatcga gctcggcggc cagctcggcc gcgagcttga tcgcggcgat gcgggtgttg
     4681 aggaattcca tcagctccag atagagctct tcctcgagct tgaatatttt ctcctcggcc
     4741 gagacgatga ggtcctcttt ctccttcagc tcgggcgtga tgaaccgctc ggcgttcacc
     4801 agggtctgct tgcggatgta gtccggcggc accaggccga ggtgggactt ggtgatctcg
     4861 atgtagtatc cgaagacctt gttgaacccg accttgaggt tgccgatccc cgtccgctcg
     4921 cgctcggttt tggcgaggcc ggcgatgtac tgccgggcgt cgcggattcc gtcgttgagg
     4981 gcgtcgaggc gcggggcgta cccccggcgg aagatgtccc ccttcaggtg ggtgagcggc
     5041 ggttcatcga ccagggcgcc ggcgaggcgg tcgacaatct cggtggtatc ggggagggcc
     5101 gccgcgagtt cggtcaatag cgggctgtgc gccgcagc
//
LOCUS       DAOPOE010000154         5026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_18172_length_5026_cov_4.278817, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000154 DAOPOE010000000
VERSION     DAOPOE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18172_length_5026_cov_4.278817"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="ispH"
                     /locus_tag="PKM94_13425"
     CDS             complement(<1..856)
                     /gene="ispH"
                     /locus_tag="PKM94_13425"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOD67677.1"
                     /translation="MLKKIHIARHHGFCMGVKRAIQIAEETAQRAAGPVTILNEIVHN
                     DAVVAKFRAQGVGQAFAVDDVDGGTLIISAHGVAPAVKEAARNRGLNLVDATCPLVER
                     IYIILDKIVKNGYHVIHWGERDHDETRGVVGHAPGHITVVSSREELLALPEWADRRLG
                     LTVQTTAHMDDVAAVEELAREKWPHIEVFNTICNATTQRQNAVMDLAPQVDMVLVVGS
                     ASSANSNRLARIANAICGRGILIASAADIRDEWFAEETDIAQVGVTAGASTPDFLVEE
                     VIRRLVEIS"
     gene            complement(850..1812)
                     /gene="lipA"
                     /locus_tag="PKM94_13430"
     CDS             complement(850..1812)
                     /gene="lipA"
                     /locus_tag="PKM94_13430"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOD67678.1"
                     /translation="MQKVFFTQSVGDRSAHAPSPQAPPRRERAMVPGVRLPRWMKTRA
                     GYSPNYDRIKNLLVAHGLHTVCQEAACPNIRECWEGGTATFMILGKYCTRGCNFCDVQ
                     KALPTGLDEDEPNRVAAAVAQLNLKQVVITSVTRDDLPDGGASIFARTIEALRRQDSD
                     VKVEFLIPDFRGNLDALAVVLASGVDVLAHNVETVQRLYKRVRPGMKLDRSLEILRTA
                     DRYRPRPVVKTGFMVGLGETGEEIRELLEQIYAAGCDIVTIGQYLRPSPHHLPIERFY
                     APEEFAEMAALGRAIGFGHVEAGPLVRSSYKAFEQSRRLLEQSC"
     gene            complement(1813..2433)
                     /gene="pdxT"
                     /locus_tag="PKM94_13435"
     CDS             complement(1813..2433)
                     /gene="pdxT"
                     /locus_tag="PKM94_13435"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOD67679.1"
                     /translation="MSEYGKLTVGVLALQGDFERHLYQLRRVGAKAREVRRPADLEGL
                     DGLIMPGGESTTMNILMDRFALREPLRAFAQTRPVWGTCAGMIMAAREIEDNQAGVRP
                     LGLIDITVVRNGYGRQVFSFEAHLAARLGPAATALAATFIRAPRVTRVGPGVTALAEY
                     RGAPVLVAAGRVLASSFHTELGEDTTLVEYFLRKYLLRTAAANIYS"
     gene            complement(2566..3810)
                     /locus_tag="PKM94_13440"
     CDS             complement(2566..3810)
                     /locus_tag="PKM94_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOD67680.1"
                     /translation="MTRPPRPPSAILRLLPVLLWCGLVLAALPEMPRAPGRPADRSHN
                     CRLWGIAGETPRGSALREQLLGGAASIDSLSRFDRDGWGLAYYPSAAGGGGGGSAPVC
                     VVERSAAAAIDDPRYDTAAAALIYAAPPIALAHIRRCSSGLCGIPDPHPFRREKGGRT
                     WLLAHNGTIGKAVLLDLIRPDYLAANPPQYGSNAAEWIDSDLYQIFLLQCLEDYAFAV
                     KPALGAAVERLRAAIAAPQLNFLLSDGAALWAYAEGNTLCYAAEAGDSAFAAVMSQPP
                     TVSGEGWTWLTDGDLVTLEPGAAPLVERIEQYFDGTGADGEPPALPRAMALEQNYPNP
                     FNASTVIAFELPARAAVRLAVCSALGRHVATLADGVRGAGRHEVRWDGRDDSGKPVAS
                     GVYLCRLEGAGRSAVRKMIVLK"
     gene            3984..>5026
                     /locus_tag="PKM94_13445"
     CDS             3984..>5026
                     /locus_tag="PKM94_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOD67681.1"
                     /translation="MNDPHVDTVARELGLRPSQVEAAVRLLDDDATIPFIARYRKEAT
                     DSLDEVAIASIRDRVTQLRELDKRRAAIVKSLDERGLLTPELEARLAAAATMAVLEDL
                     YLPYRPKRRTRAAIARERGLEPLAELLFAQDALDPAAEAGAFVDPAKDVATAADALAG
                     ARDIIAEWVNEDADARARLRALFARAAMIRSHVIAGRETEGAKFADYFDWEEPAAKAP
                     SHRYLAIRRGETEGVLSVRLLPPEASALAILETIFLMADNPAAEQVREAVHDSYKRLL
                     APALETELRATLKERADAEAVRVFADNLRELLMAPPLGRKAVLAIDPGFRTGCKVVAL
                     DRQGKLLDHLAVFP"
BASE COUNT          778 a         1885 c         1570 g          793 t
ORIGIN      
        1 cggaaatttc gacgagccgc cggatcacct cctcgacgag gaagtcgggc gtcgacgccc
       61 ccgcggtcac ccccacctgc gcgatgtcgg tctcttcggc aaaccactca tcccggatgt
      121 cggccgccga ggcaatcaga atcccccgcc cgcagatcgc gttggcgatc cgcgcgagcc
      181 ggttggagtt ggccgagctc gccgacccca cgaccagcac catgtccacc tgcggcgcga
      241 gatccatcac cgcgttctgc cgctgcgtgg tcgcgttgca gatcgtgttg aaaacctcaa
      301 tgtggggcca cttctcccgc gccagctctt cgaccgccgc tacgtcgtcc atgtgcgccg
      361 tcgtctgcac cgtcagcccc agccggcggt cggcccattc gggaagggcg agcagttcct
      421 cccgcgagga cacgacggtg atgtgcccgg gggcgtgtcc gaccaccccg cgcgtctcat
      481 cgtgatccct ctccccccag tggatgacgt ggtagccgtt cttgacgatc ttgtcgagaa
      541 tgatgtagat ccgctccacg agcgggcagg tggcgtcgac gaggttgagc ccgcggttcc
      601 gcgcggcctc tttcaccgcc ggggccacgc cgtgggccga gatgatgagc gtgccgccgt
      661 ccacgtcgtc cacggcgaag gcctggccca cgccctgggc gcggaatttc gccaccacgg
      721 cgtcgttgtg cacgatctcg ttgaggatgg tcaccgggcc cgccgcccgc tgcgccgtct
      781 cctcggcgat ctggatcgcc cgcttgaccc ccatgcagaa cccgtggtgc cgcgcgatgt
      841 gtattttctt cagcatgatt gctccagcag gcgccgggac tgctcgaaag ccttgtacga
      901 gctgcgcacg agcggtccgg cctcgacgtg cccgaacccg atggcccgcc ccagcgcggc
      961 catctcggca aactcctccg gcgcgtagaa gcgctcgatc ggcagatggt ggggcgaggg
     1021 ccgcaggtac tggccgatcg tcacgatgtc gcacccggcc gcgtagatct gctccagcag
     1081 ttcccggatc tcctcccccg tttcgcccag gccgaccatg aacccggtct tcaccaccgg
     1141 ccgcgggcgg tagcggtcgg cggtccggag gatctccagc gaccggtcca gtttcatccc
     1201 cggccgcacc cgcttgtaca gccgctgcac ggtttcgacg ttgtgcgcga gcacgtcgac
     1261 gccgctggcc agcacgaccg cgagggcgtc gagattgccg cgaaaatcag gtatgaggaa
     1321 ctcgaccttg acgtcagaat cctgccttcg gagggcctct atagtccggg caaaaataga
     1381 ggcgcctcca tccggcaggt catctcgcgt gaccgaggtg atcacgactt gtttcagatt
     1441 gagctgggcg accgccgccg ccacccggtt cggctcgtct tcgtccagcc ccgtcgggag
     1501 cgctttctgc acatcgcaga agttgcaccc gcgcgtgcag tacttcccca ggatcatgaa
     1561 ggtggccgtc cctccctccc agcactccct gatattggga caggccgcct cctggcagac
     1621 cgtgtggagc ccgtgggcga ccagcaggtt cttgatccgg tcatagttgg gcgaatagcc
     1681 ggcccgcgtc ttcatccacc gcggcagccg cactcccggc accatcgccc gctcccgccg
     1741 gggaggggcc tggggcgacg gcgcgtgggc tgagcggtcg ccgaccgact gggtaaaaaa
     1801 gactttttgc atctacgaat atatattagc ggcggccgtt cgcaacaagt attttcgcag
     1861 gaaatattcc accaacgtcg tgtcttcccc cagctcggtg tggaacgagc tcgccagcac
     1921 ccgcccggcc gccaccagga ccggcgcccc ccggtactcg gccagggcgg tcacccccgg
     1981 cccgacccgg gtcacgcggg gcgcccgaat aaaggtggcc gccagcgccg tcgcggccgg
     2041 gcccaggcgc gccgccaggt gcgcctcgaa cgagaagacc tgccgcccgt agccgttgcg
     2101 cacgaccgtg atatcgatga gcccgagcgg ccgcaccccg gcctggttgt cctcgatttc
     2161 ccgcgccgcc atgatcattc ccgcgcaggt gccccacacc ggcctcgtct gtgcgaacgc
     2221 gcgcagcggc tcacgcaacg caaaccggtc catgagaatg ttcatcgtgg ttgactcgcc
     2281 gcccggcatg atcagcccgt ccagcccctc gaggtcggcc ggtctccgca cttcccgcgc
     2341 tttcgccccg acccgccgga gctggtacaa atggcgctcg aaatccccct ggagcgccag
     2401 cacgccgact gtcagtttgc cgtattcact catggcggtt ccgcgttccc cgtctcatct
     2461 caggcttcag caacaactat agggcgtcaa aagtgcccga aataaagtcc cctccccgcc
     2521 ggcggtcaag cccgtcatgc gccccgaccg gtctccccgg cccccctatt tgagaacgat
     2581 catctttcgc acggccgacc gtcctgcccc ttcgaggcgg cagaggtaaa cgccgctggc
     2641 gaccggcttg ccgctgtcgt cccgtccatc ccagcgcacc tcgtgccgtc ccgccccccg
     2701 gacgccgtcg gccaaagttg cgacatggcg gccgagtgcg ctgcaaacag ccagccgcac
     2761 cgccgcgcgc gcgggcaact cgaaggcgat cacggtcgaa gcgttaaacg ggttcgggta
     2821 gttctgctcg agcgccatcg cccgcggcag ggcaggcggc tcgccgtccg cccccgtgcc
     2881 gtcgaagtac tgctcgatcc gctccaccag gggcgcggcc cccggttcga gcgtcaccag
     2941 gtcgccgtcg gtcagccacg tccaaccctc ccccgagacc gtcggcggct gcgacatcac
     3001 cgccgcaaag gccgagtccc cggcctcggc ggcgtagcag agcgtgttcc cctcggcgta
     3061 ggcccagagt gcggcgccgt cgctgagcag aaaattcaac tgcggcgccg cgatcgccgc
     3121 acgcagccgt tccaccgccg ccccgagcgc cggcttgacc gcgaaagcgt agtcctcgag
     3181 gcattgcaga aggaaaatct ggtagagatc ggagtcgatc cactcggccg cgttgctccc
     3241 gtactgcggt gggttggccg ccaggtagtc cgggcggatc aggtcaagga gcaccgcctt
     3301 gccgatcgtc ccgttgtggg ccagaagcca cgtgcggccg cccttttcgc gacggaacgg
     3361 atgcggatcg gggattccgc acagcccgga ggagcaccgc cggatatggg caagggcgat
     3421 gggcggggcc gcatagatca gcgccgccgc cgccgtgtcg tagcgcggat cgtcgatggc
     3481 cgcggcggct gagcgctcga ccacgcaaac cggcgccgac ccgcccccgc cgccgcccgc
     3541 cgccgatggg tagtacgcca acccccaccc gtcgcggtca aaccggctca ggctgtcgat
     3601 cgaggccgcg ccgccgagga gctgttcccg gagcgcgctc ccccgcggcg tctcccccgc
     3661 gatcccccag aggcggcagt tgtggctgcg gtcggccggc cgacccggcg cgcgcggcat
     3721 ttccggcagc gccgcgagca ccagcccgca ccagagcagg accggaagaa gacggagaat
     3781 agccgagggc ggtcgcggcg ggcgtgtcat acgcccatca taggtcattc gctccctccc
     3841 ggcaaccgat ttgggcggga tcggatcccc cggcccggcg gcgattttcg atgctcccct
     3901 ctttcctctg gcccgccggt gtcgtatttt gcgggcaacg gaccgggcgg cggcactctc
     3961 cgacccgggc cgggatggtc cctatgaacg atccgcatgt cgacactgtc gcccgcgaac
     4021 tcggcctccg gccctctcag gtcgaggccg ccgtgcgcct gctcgacgac gacgcgacta
     4081 tcccgttcat cgcccgctac cgcaaggagg ccacggacag tctcgatgaa gtcgcgatcg
     4141 cttcgattcg cgaccgggtc acgcaattgc gcgagctgga caagcggcgc gcggcgatcg
     4201 tcaaatcgct cgacgaacgc ggtctcctca cgcccgaact cgaagcccgc ctggccgcgg
     4261 ccgcgaccat ggcggtgctt gaggacctct accttcccta ccggccgaaa cgccgcaccc
     4321 gcgccgccat agcccgcgag cgcggcctcg agccgctggc ggaactgctc ttcgcccagg
     4381 acgccctcga tccggcggcc gaggccgggg cctttgtcga ccccgcgaaa gacgtcgcaa
     4441 ccgccgccga cgccctcgcg ggggcccgcg acatcatcgc cgagtgggtg aatgaggacg
     4501 ccgatgcccg cgcccgcctc cgcgcactct tcgcgcgcgc ggcgatgatc cgctcgcacg
     4561 tcatcgccgg ccgggaaacc gagggggcca aattcgccga ctatttcgac tgggaggaac
     4621 ccgccgccaa agccccgtcg caccgctacc tcgccatccg ccggggggag accgagggcg
     4681 tcctctcggt gcgcctgctt ccccccgagg cttccgcgct cgccatcctc gaaacgatct
     4741 tcctcatggc cgacaatccc gcggccgagc aggtacgcga ggcggtgcac gactcctaca
     4801 agcgtctcct tgcccccgct ctggagactg agctgcgcgc taccctcaaa gagcgcgccg
     4861 acgccgaggc ggtccgcgtc ttcgccgaca acctccgcga actgctgatg gctccgcctc
     4921 tcgggcgcaa ggcggtgctc gctatcgacc ccggctttcg caccggctgc aaagtcgtcg
     4981 ccctcgaccg ccagggcaaa ctcctcgacc acctcgccgt cttccc
//
LOCUS       DAOPOE010000155         4978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_18405_length_4978_cov_4.869185, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000155 DAOPOE010000000
VERSION     DAOPOE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18405_length_4978_cov_4.869185"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1195..2259)
                     /locus_tag="PKM94_13450"
     CDS             complement(1195..2259)
                     /locus_tag="PKM94_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67682.1"
                     /translation="MMRQLLSCLALIILFFPPAAPSRGQSAPGNHVAAELKTTDEVLE
                     QAREILPAVSPVPVKVSYESAVNLQAQAHEEYRLGPDPGRWEKALLLTIQARAKAQEA
                     IAQSASLGGPTTPDETALQRRLERADELMAQAREALEPSADNSLQALYESARSKLDRA
                     HGLCGERQYRAALKLAGQVERTAERVLQAATRAARDDFNYDRCRENVRRYFEQAGEQI
                     AECESPSALLPMEQARQAMDMSEKQASEGDRAGALHALQTARELAAQALRECTGNDNL
                     HHRHKTLRQQADRLGEAIGAENTDAFAFLAQARTQLDLAQAALEQGDRQAVVAALKAA
                     QLSLDEARRKTLAGRRTVGG"
     gene            complement(2422..3747)
                     /locus_tag="PKM94_13455"
     CDS             complement(2422..3747)
                     /locus_tag="PKM94_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD67683.1"
                     /translation="MANILVVDDEPKMTSLICGALEDAGHRVATTTRPAEALRLIESH
                     SFDIVITDLSMPEISGMQVLEKALEKEGTEVIMITAFGSIESAVRAMKKGAADYLLKP
                     FALDELELQVARLVEKQHLRSLSGHYEQEARRQVAVFVGSAPTIRAVVDMIEKVAPTD
                     ATVLISGRSGTGKEVAARMIHDRSRRAAQPFIAVNCAALTETLLESELFGHEKGAFTG
                     AVARKRGRFELAQGGTIFLDEIGEMSPGLQSKLLRVIEERQLVRVGGVDQVDIDVRVI
                     AATNRDLRSMIEKGRFREDLYFRLNVFPIRLPDLNERPEDIVPLAEHFLAEKGYPERT
                     IPPEVAHLLESYDWPGNIRELRNVIERATILAGGEPLTPEDFSLPVDDSPLTVPASSM
                     AAAGLPAAERQIIMDAIERARGNKTEAARLLKISRRRLYSRMKVHGIKP"
     gene            complement(3750..>4978)
                     /locus_tag="PKM94_13460"
     CDS             complement(3750..>4978)
                     /locus_tag="PKM94_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD67684.1"
                     /translation="ILDRQLGRRLETIAATGASLLDPGVVESLAEGNFEAYAIVSDLL
                     EDIRSASSLTELFIVDQNLDYLVTTSITEDSAYLVFEPNRPYITSLLFGLAEIPLSTP
                     SYRTGDVWLKSAYAPLYDSTGIVAAVLGVEADVDYTDTLTNLRSSLYYTTGLTAAIGV
                     LLGVIFLLLQRRFNRMQRRLFLNETQSFMGRMVAVVAHEVKNPLSIMRASAERLRKKQ
                     PAPESDFIVEEVDRLNDIVTGYLDFAAGGRESFVGHESPETLDAAELVAALKQHLAQH
                     YRQNEVQWLEYPIPPGLVFTGYRRSLRQVLLNLLINGADACREAQKPIVLGLGIRRSH
                     DAIELRVVDQGRGIPRRELGLIFEPFYTTRQSGSGLGLYLSRKIVEEMGGRLTVTSEP
                     GRKTEVVVRLPQPAGE"
BASE COUNT          881 a         1620 c         1570 g          907 t
ORIGIN      
        1 agaccgatca taaactacat caacacacaa aattgtcaag aactttcttc aacaatttac
       61 acaaatcact aaatacgata ggctatagta ataaaaaccc ccaagtgccc atgtgtccct
      121 cagcgtcgcc ggaatagccc atcaccccca aaagcgaagc cttcagggcg tcttgaagac
      181 cttccagcca ccggctcgtt atcatatccg acgggtcccg gtcaagacaa ggggtcggct
      241 accccggcgc cacaggagcg cctcggcctc tactgggaac cagttccgag gggacgtctc
      301 gtcgagcaaa agacctctag actgctcgca caagaagcat tgattgtgaa ataagtcaca
      361 aaatcccctt acctgcgcaa caccagccgg gcattccgac agccgcctgc tcatctatcc
      421 ggacgacccc tgtgagatcc tttcgagcgc ccggccaaag acgatcgtca gaccggctga
      481 ccggacccgg gcgggggaga ggtcgccacc gaaagtttcg gcgggggcgg cgtccaagtg
      541 gctgtcaccc tccgttccgt accctcgccc gtcggcccgg tggcctcccc aggatcgatt
      601 cgtgggtggc gtcggaaaac tcgccgatga tagtccggga tcggttctta cccggctgag
      661 agcgtcctga gcggccggcc gagtccggtc atgttgactc agcagaccgc ccccaggggt
      721 ttgaacaaat ttccgcgaac cccctgctga tggttcttcc agcacactcc gcaacccgaa
      781 gtgtgtcata attgacacag ccaaaagacc ccgctgtcgt cctgctgtga cgaccagaaa
      841 agtgggggcg cgaccaccca ttcccgcggc tgagccatcg ggcggatcag ccgcaatgcc
      901 cgtgggtgat gaaccatggg ccgagaccga actcaactgc acgccggcga ggggtgcaga
      961 agacctcgga agcagccgaa cgggtcacgg ctgccggctc ccgaagtgtg ggcggaactg
     1021 ccgattgctc ggtaccggaa agtaggctgg gttacgcctt aatcgtccag agtccggtat
     1081 caggttgttc ctgaattggg gaaagcaccg cccgaaggaa atggccttga cccgtgttcg
     1141 gccggctgaa agctcgcgaa agacttgcgc cgatccggtg tccgctgcaa tatatcatcc
     1201 ccccacagtc cgcctgcccg cgagggtttt tcgccgggcc tcgtccagac tcaactgggc
     1261 cgctttgagg gcggccacga cggcctggcg gtcaccttgt tcgagggcgg cctgggccaa
     1321 atcgagctgt gtccgggctt gggcgagaaa ggcgaaggca tcggtattct cggccccgat
     1381 cgcctcaccc agccggtccg cctgctggcg cagagtcttg tgccggtggt ggaggttgtc
     1441 gttaccggtg cattcccgca gcgcctgcgc ggcgagctcc cgggccgtct gcagtgcgtg
     1501 cagagcgccc gcccgatccc cctcagaggc ctgtttctcg ctcatatcca tggcctggcg
     1561 ggcctgttcc atgggcagca gggcggatgg agactcgcat tcggcgatct gctccccggc
     1621 ctgttcgaag taccgccgca cgttttcgcg gcaacggtcg tagttgaaat catcgcgggc
     1681 cgcccgcgtg gcggcctgga gaactctctc ggctgtccgc tccacctggc cagccagttt
     1741 cagggcggct cgatactgcc gctcgccgca cagcccgtgc gcccggtcaa gcttggagcg
     1801 ggcgctctcg taaagggcct gcagcgaatt gtcagcgctt ggttcgagag cctcccgcgc
     1861 ctgcgccatc aactcatcgg cccgctccag tcgccgttgt agggccgtct cgtccggcgt
     1921 cgtcggcccg ccgagagacg cgctttgggc gattgcctcc tgggctttcg cgcgggcctg
     1981 gatcgtgagc agcagcgcct tctcccagcg gcccggatcg ggccccagcc ggtattcctc
     2041 gtgcgcctgc gcctgcaggt tcaccgccga ttcataggac acctttacgg gcaccggtga
     2101 aaccgccggc agaatctcgc gtgcctgttc gagaacttca tcggtcgtct tcagttcggc
     2161 ggcgacatgg ttgcccggcg ccgactgccc ccgtgagggg gcggcgggtg gaaagaagag
     2221 gatgatcaga gcaagacaac tcaataactg acgcatcata ggcgtttctc cggcgaaccg
     2281 acgctcatcc tcctatcaaa ccctgtgccc ggcctgcctt atcgaatcat cgccttgtct
     2341 cataactgct tgcaccgtgt cccgatattc ccggtcacgc gccgccgtac cgtacggtac
     2401 aggtatgccc cgaatggcgc atcagggttt gattccgtgg actttcatgc ggctgtagag
     2461 acgccgccgg gagatcttga gcagcctggc ggcctcggtc ttgttgccgc gggcgcgttc
     2521 gatcgcgtcc atgattatct ggcgctcggc cgcagggagt cccgcggcgg ccattgagga
     2581 ggccggcacg gtcagcggcg aatcatcgac aggcagggag aagtcctccg gggtcagcgg
     2641 ctcgccgccg gccaggatcg tggcccgctc gatcacgttg cgcagctcgc ggatgttgcc
     2701 gggccaatcg taggattcca gcaggtgcgc cacctccggc gggatcgtcc gctccgggta
     2761 ccccttttcg gccaggaagt gttccgccag cggcacaatg tcctcggggc gctcgttgag
     2821 gtcgggcagg cggatcggga atacgttcag acggaagtag aggtcctcgc ggaaccgtcc
     2881 cttctcaatc atgctgcgca ggtcgcggtt ggtggcggcg atgacgcgca cgtcgatgtc
     2941 gacctggtcg acgccgccga cccggacaag ctgccgttcc tcgataacgc ggagcagttt
     3001 cgactgaagg ccgggggaca tctcgccaat ctcgtccaga aaaatggtgc ctccctgggc
     3061 gagctcaaac cgtccccgct tccgggccac cgcgcccgtg aacgccccct tttcgtgccc
     3121 gaagagctcc gattcgagga gcgtctccgt gagcgccgcg cagttgacgg cgatgaaggg
     3181 ctgggcggcc cgccgggagc ggtcatgaat catccgcgcg gctacttcct ttcccgtgcc
     3241 ggagcgcccc gaaatgagca cggtggcgtc ggtcggcgcc actttctcga tcatgtccac
     3301 aaccgcgcgg atggttgggg cggatccgac aaacacggcg acctggcgtc gcgcctcctg
     3361 ctcatagtgg cccgacaacg agcgaaggtg ctgcttctcg actaagcgcg cgacctgcag
     3421 ttccagttcg tcgagggcaa acggtttgag cagatagtcg gcggccccct ttttcatggc
     3481 ccggacggcc gactcgatgc tgccgaaagc ggtgatcata atcacctcgg tcccctcctt
     3541 ttcgagcgcc ttttcgagca cctgcatacc cgagatctcg ggcatggaga ggtcggtgat
     3601 gacgatgtcg aaggagtggg attcgatcag gcggagcgcc tccgccggcc gcgtcgtcgt
     3661 cgccacccga tgcccggcgt cctcgagagc gccgcagatg agggaggtca ttttcggttc
     3721 gtcgtcgaca accaggatgt tggccacggt cactctccgg caggttgggg cagtcgaacg
     3781 accacctcgg tttttcggcc cggctcggac gtcacagtca gccggccgcc catttcttcg
     3841 acaatcttcc ggctcaggta caacccgagc cccgatccgg actggcgcgt ggtgtagaac
     3901 ggttcgaaga tgagccccag ttcccggcgg gggatccccc ggccctggtc gaccacgcgc
     3961 agttcgatcg cgtcgtggct ccgccggatc cccagtccga gcacaatcgg tttctgcgcc
     4021 tcgcggcagg cgtcggcgcc gttgatcagc aggttgagca gcacctggcg cagcgaccgc
     4081 cggtagccgg tgaaaacgag ccccggcggg atcgggtact cgagccactg cacctcattc
     4141 tgccggtagt gctgggcgag gtgctgcttg agcgcggcga ccagttccgc cgcatcgagc
     4201 gtctcggggg actcatggcc gacgaagctc tcccgccccc cggcggcgaa atcgaggtag
     4261 ccggtgacga tgtcgttgag gcgatcgacc tcctcgacga tgaaatcgct ctccggcgcc
     4321 ggctgcttct tgcggaggcg ctccgccgag gcccgcatga tcgacagcgg attcttcacc
     4381 tcgtgggcca ccaccgccac catgcgcccc atgaacgatt gcgtctcgtt caggaacagc
     4441 cgccgctgca tgcggttgaa acggcgctgg aggagcagaa agatcacgcc gagcaggaca
     4501 ccgatcgcgg ccgtgagacc ggtcgtatag tacaggctgc tgcgcagatt ggtcagggtg
     4561 tccgtgtagt cgacatcggc ctccaccccc agcaccgccg ccacaatccc cgtgctgtcg
     4621 tacagggggg catacgccga tttcagccac acatcgcccg tccggtacga aggagtcgag
     4681 agcggaatct ccgccagccc gaaaagcagg gaggtgatgt atggccggtt cggctcgaac
     4741 accaggtagg cgctgtcctc ggtgattgag gtcgtgacca ggtagtccag gttctggtcg
     4801 acaatgaaca gctcggtgag ggacgaggcg ctgcggatat cctcaagcag atcggagacg
     4861 atcgcgtagg cctcgaaatt cccctccgcg agactctcga caaccccggg atcgagcagg
     4921 gaggcgccgg tcgcggcgat ggtctcgagg cggcggccga gctggcggtc gaggatgt
//
LOCUS       DAOPOE010000156         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_18638_length_4930_cov_5.070769, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000156 DAOPOE010000000
VERSION     DAOPOE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18638_length_4930_cov_5.070769"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKM94_13465"
     CDS             complement(<1..689)
                     /locus_tag="PKM94_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOD67685.1"
                     /translation="MNLTLYVWRQKDGRSKGRMERYEAKNVNEHMSFLEMLDVVNEEL
                     IEKGEEPIAFDSDCREGICGTCGMMINGIAHGPQRGTTVCQLHMRFFKDGDQIYIEPF
                     RSRAFPIVKDLVIDRSALDRVIAAGGFVSVNTGAPPDGNAVPISKKDADTAFDAAACI
                     QCGACVAACKNASAMLFVGAKVSHLANLPQGRVEAAERVRRMVWQMDREGFGNCTNYF
                     ECSAACPKEIPV"
     gene            complement(689..2602)
                     /locus_tag="PKM94_13470"
     CDS             complement(689..2602)
                     /locus_tag="PKM94_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOD67686.1"
                     /translation="MKLDSKVPSGPLAEKWDKHQFESKLVNPANKRKFSVIIVGSGLA
                     GASAAATLGELGYNVAVFCYQDSARRAHSVAAQGGINASKNYQNDSDSVYRLFYDTIK
                     GGDFRSREANVYRLAQVSENIIDQCVAQGVPFARDYGGYLDNRSFGGAQVSRTFYARG
                     QTGQQLLIGAYQAMMRQVGRGQVKLYPRTEMLDLVLVDGHAKGIVVRDMIGGGIYSHS
                     ADAVVLATGGYANVFHLSTNAKGCNVTATYRAYKRGAAFANPCFTQIHPTCIPVSGEY
                     QSKLTLMSESLRNDGRIWVPRQAGDSRGPNQIPEADRDYFLERKYPSYGNLAPRDISS
                     RSAKEVCDEGRGVGPGGRGVYLDFRDAIKRLGKDVIADRYGNLFDMYAKITGENPYES
                     PMRIYPASHYTMGGLWVDYNLESTIPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     ILPATISHYFARTGAKNIGIDRPEFRAVEQEAADRIRKLLAIKGKRSPDAFHREMGKL
                     MWEKCGMGRNATGLREALAKLPEIREEFWRNLSVPGDGEELNQALEKAGRVADFLEFA
                     EVLCWDALTREESCGAHFREEHQTPDGEALRNDADFAHVAAWEYKGVGVKPARHVEPL
                     AFEFVELATRSYK"
     gene            complement(2606..3340)
                     /locus_tag="PKM94_13475"
     CDS             complement(2606..3340)
                     /locus_tag="PKM94_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOD67687.1"
                     /translation="MATNQISRVTDTKVIPEPPTMTSIVRKFWMAVSGVIILTFVVGH
                     MLGNLQVFLGPDQINAYAQKLRAVPALLWAVRVFFLVWALVHVVDGAVLWLRNRQSRP
                     QTYVKQRFQEATLASRTMIWTGLAIFFFIVYHLLHYTLFVTNPEYAGLTLPDGHHNVY
                     AMLVQGFQNPLISVVYILAIFFLAVHLTHSISSFFQTFGWTRTELQPIFRGIAWTAAA
                     IIFIGYVSIPIGVLTGIIAPMAERGF"
     gene            3575..4165
                     /locus_tag="PKM94_13480"
     CDS             3575..4165
                     /locus_tag="PKM94_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67688.1"
                     /translation="MVFRILMTAVAAVLLCAGLGSAGTVGVKLSGDGVLNDTTVKAGL
                     PVSVDFYIENDSLFTGFAFGFALTSPDSSVKRVIHVADSGNGLNPAGDIKGFNGWQDQ
                     SVWNFGGVYAVESDWNGEMPELLGFGGLCVQNEYKPHPYQKVLSFRMIVPETGLMQVD
                     SAFYPPGGYWMLATPNPDATQEPAWKGPLVFRVVKE"
     gene            4306..>4930
                     /locus_tag="PKM94_13485"
     CDS             4306..>4930
                     /locus_tag="PKM94_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67689.1"
                     /translation="MAVTRNHRGGVCLRGRSLLAVVVLLGAAHADPVIRLVPETCDSS
                     GGPPAAVVTCEPPAAPGALAFFNLAIVYDSTLLQCQSVAAADGAEAAWRFSWFQETAP
                     SLGPTYWSAKILGRRPAGYVPGAGPRDSVLVRIIFRPVAPPGTQPYAPVWFYWGACDD
                     NLLVKAGDPDLLVSLDVRDPTGADITSERIPPPTTAGLCDDCFGQLAV"
BASE COUNT          815 a         1693 c         1521 g          901 t
ORIGIN      
        1 acggggatct ctttcggaca ggccgccgag cactcgaagt aattcgtgca gttgccgaac
       61 ccctcgcggt ccatctgcca gaccatccgg cgcacgcgct cggccgcctc gacccggccc
      121 tgcggcaggt tggccaggtg cgacaccttg gcccccacga agagcatcgc cgaggcgttc
      181 ttgcaggccg cgacgcacgc gccgcactga atgcaggccg ccgcgtcgaa agccgtgtcg
      241 gcgtccttct tcgagatcgg gacggcgttg ccgtcgggcg gcgcgcccgt gttgaccgac
      301 acgaagccgc cggcggcgat cacccggtcg agcgccgagc ggtcgatcac gaggtccttg
      361 acgatcggga aggcgcggga gcggaagggc tcgatgtaga tctgatcgcc gtccttgaag
      421 aaccgcatgt ggagctggca cacggtcgtg ccccgctgcg gtccgtgggc gataccgttg
      481 atcatcatcc cgcacgtccc gcagatccct tcccggcagt cgctgtcgaa cgcgatcggc
      541 tcctcgccct tctcgatcag ttcctcgttg accacgtcga gcatctcgag gaacgacatg
      601 tgctcgttga cgtttttcgc ctcgtagcgt tccatccgcc ctttcgagcg gccgtccttc
      661 tggcgccata cgtaaagtgt gaggttcatt acttgtaact ccttgtcgcc agctcgacga
      721 actcaaacgc gagcggttcc acgtgccgcg ccggtttgac gccgacgccc ttgtactccc
      781 aggctgcgac gtgggcgaag tccgcgtcat tgcggagcgc ctcgccgtcg ggcgtctgat
      841 gctcctcgcg gaaatgcgcc ccgcacgatt cttcgcgggt cagcgcatcc cagcacagga
      901 cttcggcgaa ctccagaaaa tcggccaccc ggccggcttt ctccagcgcc tggttgagct
      961 cttcgccgtc ccctggcacc gacaggtttc gccagaattc ctcgcggatc tcggggagct
     1021 tggccagcgc ttcgcgcagg ccggtcgcgt tgcggcccat gccgcacttc tcccacatca
     1081 gcttccccat ctcccggtgg aacgcgtcgg gcgagcgctt ccccttgatg gccagcagct
     1141 tcctgatccg gtcggccgcc tcctgttcca ccgcccggaa ctcgggccgg tcgatcccga
     1201 tattcttcgc cccggtccgg gcgaagtagt gggaaatggt cgcgggcaga atgaaatacc
     1261 cgtcggccag cccctgcatg agcgcgctcg cgcccagccg gttggccccg tgatccgaga
     1321 agttcgcctc cccgatcacg aacagccccg ggatcgtgct ctccaggttg tagtccaccc
     1381 acagcccgcc catggtgtag tgggaggccg ggtagatgcg catcggcgac tcgtacggat
     1441 tctccccggt gatcttcgcg tacatgtcga acaggtttcc gtaccggtct gcaatcacgt
     1501 ctttgccgag ccgcttgatg gcatcgcgga aatcaaggta cacgccccgg ccgcccggtc
     1561 cgaccccccg cccctcgtcg cacacttcct tggccgatcg cgaggaaata tcgcgtggcg
     1621 cgagattgcc gtaactcggg tacttccgct ccaggaaata atcccggtcc gcctccggga
     1681 tctggttcgg cccgcggctg tcgccggcct gccgggggac ccagatccgc ccgtcattgc
     1741 gcagcgactc cgacatgagc gtcagcttcg actggtactc tcccgacacc gggatgcagg
     1801 tcgggtgaat ctgcgtgaaa cacgggttgg cgaacgcggc cccgcgcttg tacgcgcggt
     1861 aggtcgccgt cacgttgcac cccttggcgt tggtcgagag gtggaagacg ttcgcgtagc
     1921 cgcccgtggc cagcaccacc gcgtcggccg agtgcgaata gatcccgccg ccgatcatgt
     1981 cccgcaccac gatccccttc gcgtggccgt cgaccagcac gaggtcgagc atctccgtcc
     2041 gcgggtacag cttcacctgc ccccggccga cctgccgcat catcgcctgg taggccccga
     2101 tgagcagctg ctgccccgtc tggccgcgcg cgtagaatgt ccgcgacacc tgcgccccgc
     2161 cgaacgaccg gttgtccagg tagccgccgt agtcgcgggc gaacggcact ccctgggcca
     2221 cgcactggtc gatgatgttt tcgctcacct gggccagccg gtagacgttc gcctcgcgcg
     2281 agcggaaatc cccgcccttg atggtgtcgt agaacagccg gtacaccgaa tcgctgtcgt
     2341 tctggtagtt cttcgaggcg ttgatgcctc cctgggccgc cacgctgtgc gcccggcggg
     2401 cgctgtcctg gtagcagaat acggccacgt tgtaccccag ttcgcccagg gtggccgccg
     2461 cgctggctcc ggccagcccc gacccgacga tgatcacgct gaacttccgc ttatttgccg
     2521 ggttgaccag cttgctctcg aattggtgct tgtcccactt ctcggccagc ggccccgatg
     2581 gtactttgct gtcaagcttc atgcctcaaa accccctttc cgccatcggc gcgatgatcc
     2641 ccgtcagcac gccgatcggg atgctgacat agccgatgaa gatgatggcc gcggccgtcc
     2701 aggcgatccc gcggaaaatc ggctgcagct ccgtgcgcgt ccagccgaac gtctggaaga
     2761 aactcgaaat ggagtgggtg aggtgcacgg cgaggaagaa gatcgcgaga atgtacacaa
     2821 ccgagatgag cgggttctgg aacccctgca cgagcatcgc atagacattg tgatggccgt
     2881 ccgggagcgt cagaccggca tactccgggt tcgtcacaaa cagcgtgtag tggagaaggt
     2941 ggtagacgat gaagaagaag atcgccagcc ccgtccagat catcgtccgc gaggccagcg
     3001 tcgcctcctg gaaccgctgt ttgacatagg tctgcggccg cgactggcgg ttgcggagcc
     3061 acagcacggc gccgtcgacc acgtgcacca gcgcccagac gagaaagaac acccggaccg
     3121 cccacaagag cgccggaacc gcccggagct tctgcgcgta ggcgttgatc tggtcgggac
     3181 ccaggaacac ctggaggttc cccagcatgt gcccgaccac gaaagtaagg ataataaccc
     3241 ccgagacggc catccagaac ttgcgcacga tcgaggtcat ggtcggcggc tcggggatga
     3301 cttttgtgtc ggtgacacga ctgatctgat ttgtcgccat cggccctcct ttcctgtcgc
     3361 gaagatacag ccgtccgccc cggacggtca aacaaaaaga caatcggacc gcgcgcggga
     3421 gattggccgc ccgcgcggac gattttcgcc cggccgggta taagacatac agccgtgaga
     3481 atccgtttga caagccccgg cggctgacct atgttgagaa tcatgcgaag agatcacaac
     3541 tgtttcagat acagtagctt ttgaggagat tccaatggtg ttccgaattc tcatgaccgc
     3601 ggttgccgcc gtgctgctct gcgccgggct gggctcggcc ggcactgtcg gagtcaagct
     3661 ctcgggcgac ggcgtcctca atgacacgac cgtcaaagcc ggtctcccgg tctccgtcga
     3721 cttctacatc gagaacgact cgctcttcac cggtttcgct ttcggcttcg ccctgacctc
     3781 gccggattcc tccgtcaaac gggtgatcca cgtcgccgac agcggcaacg gcctcaaccc
     3841 cgccggcgat atcaaggggt tcaacggctg gcaggatcag tcggtctgga atttcggcgg
     3901 cgtctatgcg gtcgagtccg actggaacgg cgaaatgccg gaactgctcg gtttcggcgg
     3961 cctctgcgtt cagaacgagt acaagccgca tccgtaccaa aaggtgctct cgttcagaat
     4021 gatcgtgccc gaaaccggcc tcatgcaggt ggattccgcg ttctacccgc cgggcgggta
     4081 ctggatgttg gcgaccccga acccggacgc cacgcaggag cccgcctgga aaggcccgct
     4141 cgtgttccgc gtcgtcaaag aatagggtaa gtcagagcgc cggcccggcg gttccccgcc
     4201 gggccgattc tataacctat aggtccgggc gccgaggcgg agcacacccg cgaccgctca
     4261 gatcctgttc gctgccaggc acccgagggg aaagggatgt cagccatggc cgttactcgg
     4321 aaccatcggg gcggagtctg cctgcgcggg cggtctctgc tggccgttgt cgtgctgctc
     4381 ggggccgccc acgcggaccc ggttattcgc ctggtcccgg agacgtgcga cagctccggc
     4441 ggcccgccgg ccgccgtggt cacctgtgag ccgcccgccg cgccgggggc gctggccttc
     4501 ttcaatctcg ccatcgtcta cgattccacc ctgctccaat gccagagcgt cgctgccgcg
     4561 gacggcgcgg aggcggcctg gcgcttttcc tggttccagg agaccgcgcc gtcgcttgga
     4621 ccgacgtact ggagcgccaa gatccttggc cgccgcccgg ccgggtacgt ccccggcgcc
     4681 ggtccgcgcg acagcgtcct cgtccgcatc atctttcgcc ccgttgcgcc gccgggaacg
     4741 caaccgtatg cccccgtctg gttctactgg ggcgcctgcg acgacaacct tctggtcaaa
     4801 gccggggacc ctgaccttct ggtctccctg gacgtgcgcg atccgaccgg cgccgacatc
     4861 acctccgaga ggatcccccc gccgaccacc gccggtctgt gcgatgactg cttcggccaa
     4921 ctggcggtcg
//
LOCUS       DAOPOE010000157         4731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_19682_length_4731_cov_3.685629, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000157 DAOPOE010000000
VERSION     DAOPOE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19682_length_4731_cov_3.685629"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PKM94_13490"
     CDS             <1..1166
                     /locus_tag="PKM94_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67690.1"
                     /translation="RAAADPEREQRIAPVCNNERRFYLAAGFAAGFKGMNHYMFVDRE
                     HWYGAPLHHDGTVSDGYRVIQRFNTAITEIGLEEMESRPQIAVVGNRLYSWLQLTDTS
                     KSFAYLPRLLNESTVGFCRDLTRLKLNFGVRENRDWSTMRDYKVLFIPSAEVMAQRDQ
                     EAIVELVKNGQTVIMCGLMPKYDEHFRDCKILSNHFRIKTTVDTRIGTITCKDGAEFP
                     AYLYGSIAAGDDAKTRKTAKCGAKVVGVTSARSKGNFIFFAFDIASGGNHKKLAFIEA
                     VLESEKQVSHLYCSDPSVDVAFHLGSKKSLLYVVAPPAGELSDGFEAKTKEVIIRADL
                     KGLGFGAVKVKLTDLFGDPENPTVLKSSAKELQEGLPLRLSFPDGLVFLVEKR"
     gene            1180..2883
                     /gene="ggt"
                     /locus_tag="PKM94_13495"
     CDS             1180..2883
                     /gene="ggt"
                     /locus_tag="PKM94_13495"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOD67691.1"
                     /translation="MRRGGMRRRLGLAAVLLFGVVGPACDGIRVTRLYQEGAAVTAAP
                     LATQVGEEIFRRGGNAFDAAVAIGFALAVVHPQAGNLGGGGFAVIRSAQADTVRTLDF
                     RETAPRAATGGMYLADSVTVIAEASTVGALAAGVPGTVAGLYALWQAYGSMPWEELVR
                     PAALLADKGFPVDAFLANSLRAYEHQLSRFDETREVFFSDGRLPAAGDTLVQPDLAQT
                     LYLIAAQGPEGFYAGQVADSIAATMQKYGGLITREDLAEYRAAWRDPVYFRFDSLDCY
                     SMAPPSSGGLMVGQILKLLEPHSFSGWRPDSPAYMHLFAEAARLAYADRAEYLGDPAF
                     TKAPGNLLDPGYLAQRRLQIDTLHATPSESVSPGLAPAPESDQTTHYSVCDAEGNMVA
                     VTYTINTSYGSALVVGGAGFLLNNEMDDFAIAPGVPNTYGLVGGAANRIEPGKRMLSS
                     MAPLLVLVDGRPFLILGSPGGSRIITTVAQTLLNFTRFKFPLTEALAQARFHHQWLPD
                     TLYVETGGYDAATVRALAGYGHAVKEREAFGDVQAIFIDDRGYMSGASDPRGRGTAGG
                     L"
     gene            complement(2916..4244)
                     /locus_tag="PKM94_13500"
     CDS             complement(2916..4244)
                     /locus_tag="PKM94_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67692.1"
                     /translation="MPRTAHHTPGRATRLLLRAVFTIVLLTVSLAGAASGMVFLRQEN
                     LHISNLHRIEEDVYAYSQNMTVDGFVDGELTAFVYSLQINGDVTGSANVLAYQLTHRG
                     HIERSLRGFASTVIIDGSVGRSLLLAANEVTINRAAVVGRDARIYGNNVRLYGTITGN
                     TYVSGSSLIIEGTIEGDLDLRAAKRAEIASTAVITGNLTYRSPSEGDLVIADGATVAG
                     TVTWVPPAEEAKDGEPSPWTAGPILCVAKLLAAFLFGIILVAVFRRHAEESLNQLRAR
                     LVVSIAVGFLVLIGFTLAALTFIIALVLVITGLILVSGQGVVLGAFLVIFSTLALPIA
                     SFASVSAAIMLYSGKIVVAYLAGALLLKRARPALLSWWNLLLGLVLLFVIFEVPYLGN
                     LVYLLVGVTGAGAIALGVRNCRRPVESPAPAAPSYPPPPTPPPPPPSYPA"
     gene            4458..>4731
                     /locus_tag="PKM94_13505"
     CDS             4458..>4731
                     /locus_tag="PKM94_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOD67693.1"
                     /translation="MNVLVYLFGAILLYMLAFRYYGRYLGRQVGLQPERPTPAVEFND
                     GRDFVPTRPSVLFAHHYSAIAGAGPIIGPTLGILYGVGPVWLWVVLG"
BASE COUNT          810 a         1398 c         1759 g          764 t
ORIGIN      
        1 ggcgggcggc ggccgatccg gagcgggagc agcgcatcgc cccggtgtgc aacaacgaac
       61 ggcggttcta cctcgcggcc gggttcgcag ccgggttcaa gggcatgaac cactacatgt
      121 tcgtggaccg cgaacactgg tacggcgcgc cgctccacca cgacggcacg gtctcggacg
      181 gataccgggt gatccagcgg ttcaacacgg cgatcaccga gatcggtctg gaggaaatgg
      241 aatcccggcc ccagatcgcg gtcgtcggca accgcctgta ttcctggctg caactgaccg
      301 atacgtcgaa atcgttcgcc tatctccccc gcctgctcaa cgagtcgacg gttggttttt
      361 gccgggacct gacccggctg aagctgaatt tcggcgtgcg cgaaaacaga gactggtcga
      421 cgatgaggga ctataaggta ctcttcattc cctcggccga ggtcatggcg cagcgggacc
      481 aggaagcgat tgtcgagctg gtgaaaaacg gccagacggt catcatgtgc gggctgatgc
      541 cgaagtacga cgagcatttc cgggactgca aaatcttgtc gaatcatttc cgaatcaaga
      601 cgaccgtgga cacgcggatc gggacgatca cctgcaaaga cggcgcggag ttcccggcgt
      661 acctctacgg gagcatcgcg gccggggacg acgccaagac gcgcaagacg gccaagtgcg
      721 gggcgaaggt cgtgggggtg acctcggccc ggtcgaaagg gaacttcatc ttcttcgctt
      781 tcgacatcgc ctcgggcggc aaccacaaga agctggcctt tatcgaggcg gtgctcgagt
      841 cggaaaagca ggtgtcgcac ctgtactgct ccgatccctc ggtcgacgtg gcgttccacc
      901 tggggtcgaa gaagtcgctg ctgtacgtgg tggcgccgcc ggccggggag ctgtcggacg
      961 ggtttgaggc gaagacgaag gaggtcataa tccgggcgga cctgaagggg ctggggttcg
     1021 gtgcggtgaa agtcaagctc accgacctgt tcggcgatcc ggagaatccg acggttctga
     1081 agtcgtcggc caaggaactg caggagggac tgccgctccg cctcagtttt ccggacgggc
     1141 tggtgttcct ggtcgagaaa cgatagcggg gaatcactca tgcggcgagg tggaatgcgg
     1201 cgccggctcg ggctcgcggc ggtgctgctc ttcggggttg tcggtccggc ctgcgacggt
     1261 atccgggtga cccgcctgta tcaggagggg gcggccgtga ccgcggcgcc tctggcgacg
     1321 caggtggggg aggagatttt ccgccggggg gggaatgcgt tcgacgcggc ggtagcgatc
     1381 gggttcgctc tcgcggtcgt ccacccacag gcggggaatc tcggcggcgg cggttttgcc
     1441 gtgatccggt cggcgcaggc ggatacggtg cggacgcttg attttcgcga gacggcgccc
     1501 cgggcggcca ccggcggcat gtacctggcc gacagcgtga cggtgatcgc ggaagcgtcg
     1561 accgtcgggg cgctcgccgc gggcgtgccc ggcacggtcg ccgggctcta cgcgctgtgg
     1621 caggcctacg gctcgatgcc ctgggaagag ctcgtgcggc cggcggccct gctggcggac
     1681 aaggggttcc cggtcgatgc gtttctggcg aattcgctgc gcgcctacga acaccagttg
     1741 tcccggttcg acgagacgcg ggaggtgttc ttctccgacg ggcggctccc ggcggccggc
     1801 gacacgctgg tccagccgga tctcgcccag accctctacc tcatcgcggc ccaggggccg
     1861 gaaggtttct acgcgggaca ggtggcggac agcatcgcgg cgacgatgca gaagtacggc
     1921 ggcctgatca cgcgcgagga tctcgcggag taccgggcgg cgtggcggga cccggtgtac
     1981 tttcgtttcg actcgctgga ctgctattcg atggctccgc cctcctcggg cgggctgatg
     2041 gtcgggcaga ttctgaagct tctggagccg cactcatttt ccggttggag gccggactcg
     2101 ccggcctaca tgcacctgtt cgctgaggcg gcccggctgg cctacgccga ccgggcggag
     2161 tatctcggcg atccggcgtt taccaaggcg ccggggaatc tgctcgaccc gggctatctg
     2221 gcgcagcggc ggctgcagat cgacacactc cacgcgacgc cctcggagtc ggtctccccc
     2281 ggcctggcgc cggccccgga gtcggaccag acgacgcact acagcgtctg cgatgccgag
     2341 gggaacatgg tggcggtcac ctacacgatc aacacgtcgt acggttcggc gctggtggtg
     2401 ggcggggcgg ggtttctgct gaacaacgag atggatgatt tcgcgatcgc cccgggcgtg
     2461 ccgaacacct acgggctggt cgggggggcg gccaaccgga tcgagccggg gaagcggatg
     2521 ctgtcgtcga tggccccgct gctcgtgctt gtcgatgggc ggccgtttct cattctcggg
     2581 tcgccgggcg gctcgcggat catcacgacc gtggcgcaga cgcttctcaa cttcactcgg
     2641 ttcaagttcc ccctgacgga ggcgttggcg caggcgcggt ttcaccacca gtggctgccg
     2701 gacacgctat atgtggaaac gggagggtat gatgcggcca cggtgcgggc gctggccggt
     2761 tacgggcacg ccgtgaagga gcgggaggcg ttcggcgacg tgcaggcgat atttatcgac
     2821 gaccgcggat atatgagcgg ggcgtccgac ccgcggggcc ggggaacagc cggggggctt
     2881 taagcagcgg cacaccgaca gcgggccggc gcgaatcagg cgggataact cggcggcggc
     2941 ggcggagggg tcggcggagg cggatacgac ggggccgcgg gggcgggcga ttcgaccggg
     3001 cggcggcagt tgcgcacgcc cagggcgatg gcgccggcgc cggtcacgcc gaccagcagg
     3061 tacacgagat tccccaggta gggcacctcg aagatcacga acaacagcac caatcccagc
     3121 aggaggttcc accagctcag gagagccggg cgcgcccgtt tcagcagcag cgcccccgcc
     3181 aggtatgcga ccacgatctt gccggaatag agcatgatgg cggcagagac ggaggcgaag
     3241 ctggcgatgg gcagggcgag cgtggagaaa atcaccaaaa aagcgccgag gacgactccc
     3301 tggcccgaga ccaggatcag gccggtgatg acgaggacca gggcgatgat gaatgtcaga
     3361 gcggccaggg taaagccgat gagcacgagg aagccgacgg cgatggagac gaccaggcgc
     3421 gcgcgcagct ggttgagcga ctcctcggca tggcgccgga agacggcgac gaggatgatg
     3481 ccgaagagga acgcggcgag cagtttcgcg acgcagagaa tcggcccggc ggtccacggc
     3541 gacggctctc cgtctttcgc ctcttccgcc ggcggcaccc aggtcacggt gccggcgaca
     3601 gtcgccccgt cggcaatgac gagatccccc tcggagggag accggtaggt gagattgccg
     3661 gtgatgacgg cggtggaggc gatctcggcg cgtttggcgg cgcgcagatc gagatccccc
     3721 tcgatcgtcc cttcgatgat gagggacgac ccggagacgt aggtgttgcc ggtgatggtg
     3781 ccgtagaggc gcacattgtt gccgtagatg cgggcgtcgc gcccgaccac cgccgcgcgg
     3841 ttgatagtca cttcgttagc ggcgagcagc agcgaccggc ctacggagcc gtcgatgatg
     3901 acggtgctgg cgaacccgcg gaggctccgc tcgatgtgtc cgcggtgggt cagttggtag
     3961 gccaggacgt tggccgaccc ggtcacatcg ccgttgatct ggaggctgta gacgaacgcg
     4021 gtcagctcgc cgtcgacgaa cccgtcgacc gtcatgttct gggagtaggc gtagacgtct
     4081 tcctcgatgc ggtgcaggtt ggagatgtgg aggttttcct ggcggaggaa gaccatgccg
     4141 gaggcggcgc cggccaggga cacggtcagg aggacgatag tgaagacggc acgcagcagg
     4201 aggcgcgtcg cccggccggg tgtatggtgg gcggttctcg gcataccact gtgtctacgc
     4261 cgggaggcgg cgtaagttgc tgaatgtata ggaaacggcg gggggcaata aaagcaaaaa
     4321 aagcggccaa tgggcagggg cgcgagtgct cggcgccgaa aaatgaggcc ttgcgggaga
     4381 ggaaaaaacc ggcatattgc ggtcagagcc gctgcccggc ccgacccgcg cgccgggggc
     4441 ggcgcgaggg agcgtacatg aacgtactgg tctatctgtt cggggcaatc cttctctaca
     4501 tgctggcgtt tcgatactac gggcggtacc ttgggcggca ggtggggctg cagccggagc
     4561 ggccgacgcc cgcggtggag ttcaacgacg gccgggattt cgtgccgacc cggccctcgg
     4621 tgctgttcgc ccaccactac tcggcgatcg ccggggcggg gccgatcatc ggcccgaccc
     4681 tcggcatcct gtacggggtc ggcccggtgt ggctgtgggt ggtgctcggg g
//
LOCUS       DAOPOE010000158         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_20332_length_4607_cov_3.996046, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000158 DAOPOE010000000
VERSION     DAOPOE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20332_length_4607_cov_3.996046"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2371
                     /gene="ccsA"
                     /locus_tag="PKM94_13510"
     CDS             122..2371
                     /gene="ccsA"
                     /locus_tag="PKM94_13510"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOD67694.1"
                     /translation="MAAFGEYCILAAAAAALLSAVLYALVWRGREDYRRAARAAFTLT
                     ALFLTGALLALLHLILTHDFRIAYVFSYSSTDLPLWYLVASLWGGQEGTFLLWIVFVA
                     VLGLVLIASPRAREFEAGNLVFVNLFILSILFILLKKSPFEYLPVWRTEGNGLNPLLQ
                     NYWMTIHPPVMFVGFAAAVMPAAFAMTALVGRKYDTWAEAARPWTLFAWAALGAALVL
                     GGYWAYETLGWGGFWAWDPVENSSFIPWIFLAAQIHALFIKRQRQGLMRFSLAIVLVT
                     FWSVLYGTFLTRSGVLGDFSVHSFVDLGINNFLLGGLAGFIALGLSLLVWRWRDIRPG
                     ASFANVASRSYLVSLGVVVLFLGGLLTLAGTSAPLLTRLAGEPSAVGLSYYFATMTPV
                     AAVVLLLLGLFPSFRWNHGLSRRWLLLGGGGAAAATAAGLWLSGTTGDPLYLALFAAA
                     TWALVSNGYALASSWRKGAVNPAYLAHIGLAAALIGAAVSAGFETKQTVRLPQGETVR
                     AMGYDLTFVRTEPTAKGFDCRVEVAGAGAAFTAVLPHEFPRNMEGTMRKPHVEKFLGH
                     DIYLSPLALEQPKGPEPGSYTLAKGESAAVDKYTLTFHEFALTSHSEGAVTEAGARIT
                     VAWAGGREEILPRLRVSGDEVAALPAPFDEGRGTVAITAVRPEDGAVAIKVAGAFVPP
                     AEMAGETLTIELSEKPLINLFWLGTALVFGSGLWSMRERRRRRVTDAAGTGAAAKAAP
                     GEISREISV"
     gene            2473..2769
                     /locus_tag="PKM94_13515"
     CDS             2473..2769
                     /locus_tag="PKM94_13515"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOD67695.1"
                     /translation="MGRVSDLPPGRSLEKRVLARRIIVVNDAGIMRALEGDCKHMKAS
                     LASGPIEDGVITCRMHGWKYRLATGECLTNPAFRLKTYPVETEEGKIYVVVDHG"
     gene            2762..3400
                     /locus_tag="PKM94_13520"
     CDS             2762..3400
                     /locus_tag="PKM94_13520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOD67696.1"
                     /translation="MVEQATDLFAKVHRYLEEIAPPRPPVMREMEAYAAEHGFPIVGP
                     LVGRFLYQLAMLTKARRVMELGSGFGYSAYWFSLAMGGRGEIVMTDADRRNKRRANDY
                     FMRAGLESRFDFQVGNALRLLERQDGPFDIVFNDVDKEDYPRTIELAAPRLRKGGLFL
                     TDNAIWSGRVADGRGDAPTQAVRAFNAALVRDSRFWTTIVPIRDGVAVALRL"
     gene            3951..4382
                     /locus_tag="PKM94_13525"
     CDS             3951..4382
                     /locus_tag="PKM94_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOD67697.1"
                     /translation="MAPKRAKFFYYGDDARTEEVRKFIEDAGIMLDMRDMSQRPLTRY
                     ELSDLIGHLNIEHFINKASEAYAAHGLDGGPLDREKVLDLMAQDYTLIRKPIIVYSRL
                     LTIGYDRKKITEMFQINSNGQAEPTIPENRGNRRMAAGANR"
BASE COUNT          787 a         1379 c         1601 g          840 t
ORIGIN      
        1 gaaatttccg gcgggacggc cgaagtgatc aagccggagc ggcgccggcg gcgggggagc
       61 gacgaggcgg cgctcgacta gcccgccggc gaccgtccca cccaaagagc aggagcctgg
      121 catggcagca tttggcgagt attgcatcct ggccgcggcc gcggccgcgt tgctctcggc
      181 cgtcctctat gccctcgtgt ggcgggggcg cgaggactac cggcgggcgg cgcgggcggc
      241 cttcacgctg acggcgctgt ttctgaccgg ggcgctcctg gctctgctcc atctgattct
      301 cacccacgat ttccgcatcg cctacgtctt ctcctactcc tcgaccgatc tccccctctg
      361 gtaccttgtc gcttcgctgt ggggcgggca ggaagggacg ttcttgctgt ggatcgtgtt
      421 cgtggcggtg ctcgggctgg tgctgatcgc gagccccagg gcgcgggaat tcgaggccgg
      481 gaatctcgtc ttcgtgaatc tcttcatttt gtcgattctg ttcattctcc tgaagaagtc
      541 gccctttgag tatctgccgg tgtggcggac cgagggcaac gggctcaacc cgctgctgca
      601 gaactactgg atgacgattc atccgccggt gatgttcgtg gggttcgcgg ccgcggtgat
      661 gccggcggcg tttgcgatga ctgccctggt ggggcggaag tacgatacct gggcggaagc
      721 ggcgcggccc tggacgctct ttgcctgggc ggccctggga gcggcgctcg tcctcggcgg
      781 ctattgggcc tacgagacgc tcggctgggg gggattctgg gcgtgggacc cggtcgaaaa
      841 ttcgtcgttc attccctgga tttttctcgc ggcccagatc cacgccctct tcatcaaacg
      901 ccagcggcag ggactcatgc gcttctccct cgccatcgtg ctggtgacgt tctggtcggt
      961 cctctacgga acgttcctga cccggtcggg agtcctggga gacttctcgg tgcactcctt
     1021 cgtggacctc ggaatcaaca acttcctcct cggcgggctg gcggggttca tcgcgctcgg
     1081 gttgtcgctg ctggtctggc gctggcggga catccggccg ggagcgtcgt ttgccaatgt
     1141 cgcatcgcgc tcgtacctcg tgtcgctcgg cgtcgttgtg ctgtttctcg gcggactgct
     1201 caccctggcg gggacgtcgg cgccgctgtt gactcggctg gccggagagc cttcggcggt
     1261 gggattgtcg tactacttcg cgaccatgac gccggtggcg gcagtcgtgc ttctgctcct
     1321 gggtctgttc ccgagttttc gctggaacca cggactctcg cggcggtggc tcctgctggg
     1381 gggcggcggc gcggcggcgg ccacggccgc cggcttgtgg ctgtccggga cgacgggcga
     1441 tccgctgtac ctcgctctct tcgccgcggc gacctgggcg ctggtttcca acggctacgc
     1501 cctggcctcg tcctggcgaa aaggggcagt caaccccgcc tacctcgcgc acatcggcct
     1561 ggcggcggca ctgatcggtg cggccgtctc agccgggttc gaaaccaagc agacggtccg
     1621 gctgcctcag ggagaaacgg tccgcgccat ggggtacgat ctgaccttcg tccgcacgga
     1681 gccgaccgcc aaagggtttg actgccgtgt cgaggtggcc ggggctgggg cggcgttcac
     1741 cgccgtgctg ccgcacgagt tcccgcgcaa catggaaggg acgatgcgca aaccgcacgt
     1801 ggagaaattc ctcgggcacg acatctacct gtcgccgttg gctctcgaac agccgaaggg
     1861 accggagccg gggtcgtata cgctggccaa aggggagtcg gccgcggtgg acaaatatac
     1921 actgaccttt cacgaattcg ccctcaccag ccacagcgag ggggcggtga ccgaagcggg
     1981 agcgcgcatc acggtggcct gggccggcgg ccgcgaagaa atcctgccgc ggctgcgcgt
     2041 gagcggcgac gaggtggcgg ccctcccggc gccatttgac gaggggcggg ggacggtggc
     2101 cattaccgcg gtgcggcccg aggacggtgc ggtggcgatc aaggtggccg gcgcttttgt
     2161 gccgccggcg gagatggcgg gggagacgct cacgatcgaa ctgtcagaaa aaccgctcat
     2221 caacctgttc tggctcggaa ccgcgctcgt gttcgggtcg gggttgtggt cgatgcggga
     2281 gagaagacgt cgccgcgtga ccgatgccgc agggaccggc gcggccgcca aagcggcgcc
     2341 gggggagatt tcgcgagaaa tatccgtttg atattcggtc cctccgccgt atcttaattc
     2401 agtcgcgcac gacgcgcttc gtcagaccag gcaacccgcg cgacgcagtc acaacgcatg
     2461 gcgagaatcc ggatggggcg agtcagcgat ctgccgcccg gacgttccct ggagaaacgg
     2521 gtgctggcgc ggcggatcat cgtcgtgaac gatgcgggga tcatgcgcgc gctggaaggg
     2581 gactgcaaac acatgaaagc ttcgctggcg agcggcccga tcgaggacgg ggtcatcacc
     2641 tgccgcatgc acgggtggaa ataccggctc gcgaccggag agtgcttgac caacccggcg
     2701 tttcggctga agacttatcc ggtcgagacc gaagagggaa agatatacgt ggtagtcgat
     2761 catggttgag caagcgaccg atctgtttgc gaaagtccac cggtatctgg aggagattgc
     2821 gccgccgcgg ccgccggtga tgcgggaaat ggaggcctac gccgccgagc acgggtttcc
     2881 cattgtcggc ccgctggtgg ggagatttct ctaccagctg gcgatgctga ccaaggcgcg
     2941 gcgggtgatg gaactgggct cgggcttcgg ctacagcgcg tactggtttt cgctcgcgat
     3001 gggggggcgg ggggagattg tgatgaccga tgccgatcgc cgcaacaaga gacgggcgaa
     3061 tgactatttc atgcgcgccg ggctggagag ccggttcgac ttccaggtcg gcaacgccct
     3121 gcggctgctc gaacggcagg acgggccgtt cgacatcgtt ttcaacgacg tcgacaagga
     3181 ggattatccg cggacgatcg agctggcggc gccgcgcctg cgcaagggcg ggttgtttct
     3241 gaccgacaac gcgatctgga gcggtcgggt ggccgatggc cggggcgacg caccgacgca
     3301 ggcggtgcgg gctttcaatg ccgctctcgt gcgcgacagc cggttctgga ccaccatcgt
     3361 gccgatccgc gacggcgtgg cggtggcgct gcgcctgtga ccgggcgcgc cggggccgga
     3421 cgacagcgac agcctctgtc tgtcaggttt cgtcaggcgg aaaccgacag ccccgaaaat
     3481 tgcggtccag ccgggaaacc ttcccggctt gccggacgtt taaggagtag tcagtgcggc
     3541 tcaagtctcc gcggcgattt ccgatagaac gcttgaacgg tcattggttc cgcgaacgag
     3601 atttcataga gatcacctta gggaggagcc ggcccgccga gtggatcgcg gcgcggactt
     3661 gagcaccgaa cagagggcgc agatatcggc ggagtcggac ggggaagcgg ccggcgacga
     3721 ccggccggag tgccaacaca gaaatcagtt gacaacgggc tgtagaaaat agttagattt
     3781 acccccgtta ggctgatccg ccgagcgcgg cacaaaaacc tgacgtctcc gatccccggc
     3841 gcaactcctc gaccgcgagc agtcgccgaa ccaaagagtc aaagggtacg agcggtcgca
     3901 agacctgcga acgagaaaca ttaaggtcgt ttatttttcc aggaggaatc atggctccca
     3961 agcgcgcgaa attcttttac tacggcgatg acgcccgcac ggaagaggtg cggaagttca
     4021 ttgaagatgc cgggattatg ctcgacatgc gggacatgtc gcagcgcccg ctcacccggt
     4081 acgaactgtc ggacttgatc ggccatctca acatcgagca cttcatcaac aaggcctcag
     4141 aggcgtacgc cgcgcacggt ctggacggcg gtccgctgga ccgggagaag gtgctggacc
     4201 tgatggcgca ggactatacg ttgattcgca agcccattat cgtctactcg cggctgctga
     4261 cgatcgggta cgaccggaag aaaatcacgg agatgttcca gatcaacagc aacgggcaag
     4321 ccgagccgac gattccggaa aaccggggca accggcgcat ggccgccggc gccaatcgct
     4381 gaaacgggct tttcaattcg tcccgtcttg actatacttc cgtacgttgt agcgcggaaa
     4441 ggtgaaacgt atggaagtgc aacgcgaagt tctcgagaca gatatagcga ttgtcggggc
     4501 cggaccggcc gggctggcgc tggcctatca tctggccagg ctggctgagg ccgacggcgg
     4561 cgcggccaaa cccgagatcc tcctgatgga caagggcgcc tatgccg
//
LOCUS       DAOPOE010000159         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_20391_length_4597_cov_4.220608, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000159 DAOPOE010000000
VERSION     DAOPOE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20391_length_4597_cov_4.220608"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKM94_13530"
     CDS             complement(<1..409)
                     /locus_tag="PKM94_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67698.1"
                     /translation="MRTACFALAAAMLLATASTASGAEVPVLFSYQGRLTDNTGVPLD
                     GSYTMRFALYFDTGGTVNLWTETRPDVPVADGLFEVVLGSVTEFPDNIFSGPIRYLGI
                     TVVGYPEGRPLTPIVSVPYAYRSSLAWRAESVAD"
     gene            807..1040
                     /locus_tag="PKM94_13535"
     CDS             807..1040
                     /locus_tag="PKM94_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67699.1"
                     /translation="MTGENLFSGEAGREPREAAGAVPLFGAESSFDWMNTAVAGGGDC
                     CYLDAATCPDCSSGMIRAGACFTCPMCGWGGCN"
     gene            1137..3839
                     /locus_tag="PKM94_13540"
     CDS             1137..3839
                     /locus_tag="PKM94_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD67700.1"
                     /translation="MSVLRLEELAPYGIPRRLIELWRERQGETLLPVQSRAVRRGLLG
                     APGERAEEAAPLRMLISAPTAAGKSFCAEMAVARTLAGRRKALVLVPLRSLADQKHRL
                     LAGTFGALGLRCLIASGDYPENDRAFAESDYDVAVAIFEKFDGLLGTRLEVLRAIGLL
                     VVDEIQTVSEPGRGAVLERLLTCVRASAYRPGLLGLSAVIGEGEKAAGRLAEWLEAGL
                     VVESSRPVDLLRGVAAEGAFRYRSFNSGREASEPFVLAREPGGDLFESLIERLREEPG
                     STIVFLKSRRETVEAAMRLAGAVGWPAAEQGTAELAEEEPSALNRILRRVMARGVAFH
                     NADLSPRQRRAVEESFARREVRVIFATTTLAMGVDLPADTVYLETVKYTGGRRDARPA
                     LVPVSRAEFDNMAGRAGRAARAEVRPGRAVALAQSEFEGDILWENYIASGEYTSVRSA
                     FDSMPLADWALHVLATGLAADAAGLAAVYRRSLRAVTEPEAPPPDFEAVLDLLTREGV
                     AGREPDGLVRPTPDGETAVQTGLTCAQAVHYTRRLRESRPAEEFGWLALALSGPDWEL
                     PAGLLSRQELADNGPLRRLYRRCDHWLASARVLIGQPGGRGLTEYRTAGALKAALLLE
                     QWRRMTPVRELEEEFRVHAGQIMAIGDTAAHLLSGIAGLLDNLDGGGDKLRPLVFSVR
                     FGVVPELQGLHAAFRDILARSDFAALAQAGIVRVPDLIGHDPTDLGEIVKQERKLQKL
                     LARIMEWKKEVSMRQSEVLSRVAPYPVRGSGPRPASVEIDGSCERERYLVRVDGRPVW
                     LTGKSFKYFTRLAWSRVQRDGGWMYKEDIEQGFNQARYLYRMKNEINERLDSEWEVFE
                     NNRLGYYRLNADAGRIRLNVERLRNHPDWEVRSLFAGQTESAVN"
     gene            4163..>4597
                     /locus_tag="PKM94_13545"
     CDS             4163..>4597
                     /locus_tag="PKM94_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOD67701.1"
                     /translation="MIVRSKNKLPVLVTDQEQQYPVLSLLTGVLFPGSMITIQVGRRS
                     SIDLIRQCHERKQEFVASFTLSRRGGDDVGALHEVGVLAAVRDIQKGVGNSLIVTIEG
                     LRRAVLGEVVRQDPYLTATVLFLEDPAGPDKDIAAKVRQVIDV"
BASE COUNT          735 a         1303 c         1788 g          771 t
ORIGIN      
        1 catcggccac gctttccgcc cgccaggcaa gcgaggaccg gtaggcatag gggacactca
       61 cgatcggggt cagcggccgg ccctcagggt agcccacgac ggtgatgccc aaatagcgga
      121 tcggcccgga gaagatgttg tccgggaact cggtcacgct ccccaacacc acttcaaaca
      181 ggccgtcggc gacgggaacg tcgggccgcg tttcggtcca gaggttgacg gtgccgccgg
      241 tgtcgaagta cagggcaaac ctcatggtgt aggagccgtc gaggggaaca ccggtgttgt
      301 cggtcaggcg cccctggtag gaaaacagaa cagggacctc agcgcccgac gcggtggagg
      361 ctgtcgccag cagcatggct gcggcgaggg cgaaacaggc tgtgcgcata cgcatctcct
      421 cgttcgcgga cgtattcgaa aacgtgtgtg agcttcttct gcgttggact gacgattcaa
      481 tatagcgccg ccgaccagtt tgtccagatt caatagaaac tgccgatggg ggcgcggttc
      541 gggccgtctc ccattggatc ggtgccaagc tgtgggggaa gcagcagacc ggctgagtgc
      601 gggggaccga ccggcaggcc ggtgcttccg cgccgccgcc ccctctgccc gttcggactt
      661 tctaagccgt tgttattccc ttatttacat agataactgc tcatgcgtgc agttattctt
      721 cggccaccct gcacttttcg cttgacaggg gcacaaatgt gcagtatatg gggagaaacg
      781 gagattaatc acgtcaggag gcgaggatga caggcgagaa tcttttttcc ggcgaggcgg
      841 ggcgggagcc ccgggaggcg gcgggggcgg tgccgctgtt cggggcggag agttcatttg
      901 actggatgaa cacggcggtg gcggggggtg gcgattgctg ttacctcgat gccgccacct
      961 gtccggactg cagcagcggg atgattcgcg cgggggcttg tttcacctgc cccatgtgcg
     1021 ggtggggcgg gtgcaactga cgacgggcgg acggttgcgg gcggtgcagg gcgaggcgaa
     1081 gagggacact gagatgctga gtggccggcg gagtgcggcc ggaagcggga ggacagatga
     1141 gtgtgttacg gttggaggag ttggcgccat atggcattcc ccgccggctg attgagctct
     1201 ggcgggagcg gcagggggag acgctgctgc cggtgcagag ccgggcggtc cgtcggggtc
     1261 tgctgggcgc gccgggggaa cgggccgagg aagcggcgcc gctgcgcatg ttgatcagcg
     1321 cgccgacggc ggcggggaaa tcgttttgtg ccgagatggc ggttgcccgg acactggcgg
     1381 ggcggcggaa agcgctcgtg ctcgtgccgc ttcgctcgct ggccgaccag aaacaccgcc
     1441 tgctggcggg gacgttcggg gcgctcggcc tgcggtgtct gattgcgagc ggcgattatc
     1501 ccgaaaacga ccgtgctttt gcggaatcgg attacgacgt cgcggtggcg attttcgaga
     1561 aattcgatgg tctgctcggc acccgtctcg aagtcctgcg cgcgatcggg ctcctggtcg
     1621 tggacgaaat ccagacggtg tcggagccgg ggcgcggcgc tgtgctcgag cggctgctca
     1681 cgtgcgtgcg ggcatcggca taccggccgg gactgctcgg cttgtctgcg gtgatcggcg
     1741 aaggggagaa agcggccggg cggctggccg agtggctgga ggccgggctg gtggtcgaga
     1801 gcagccgtcc ggtcgatctc ctccgcgggg tcgccgccga aggggcgttc cgctaccgct
     1861 cgttcaattc cgggcgggag gcgagcgagc cgtttgtcct tgcgcgcgag ccgggcggcg
     1921 atctgttcga gagcctgatc gagcggctgc gcgaggaacc gggatcgacg attgtctttc
     1981 tcaagtcgcg gcgggagacg gtcgaggcgg ccatgcggct ggcgggggcg gtcggctggc
     2041 cggcggccga gcagggaacg gcggagttgg cggaagagga gccctcggcg ttgaaccgca
     2101 ttcttcgccg ggtcatggcg cgcggggtgg cgttccacaa cgcggatctc tccccccggc
     2161 agcgccgggc ggtggaggag agttttgccc ggcgggaggt gcgggtgatc ttcgccacaa
     2221 cgacgctggc catgggggtg gacctgccgg ccgacacggt gtatctcgag acggtcaagt
     2281 acaccggggg gcggcgcgat gcccggcccg cgctcgtgcc ggtgagccgg gcggagttcg
     2341 acaacatggc cgggcgggcc gggagagcgg cccgggcgga agtgcggccg ggccgcgcgg
     2401 tggccctggc ccagtcggaa tttgagggcg atatcctctg ggaaaactac atcgcctccg
     2461 gagaatacac atcggtgcgg tcggcgttcg actccatgcc gctcgcggac tgggcgctgc
     2521 acgtgctggc gaccggcctg gcggccgatg cggcgggact ggccgccgtc taccggcgct
     2581 cgctgcgggc ggtgacggag ccggaggcgc cgccccccga tttcgaggcg gttctggact
     2641 tgctgacgcg ggagggagtg gccgggcgcg aaccggacgg cctggtgcgt ccgaccccgg
     2701 acggggagac cgccgtgcag accgggctga cctgcgcgca ggcggtgcat tacacccggc
     2761 ggctgcggga gagtcggccg gccgaggaat tcgggtggct ggcgctggcg ctcagcgggc
     2821 ccgactggga gttgcccgcg ggactgctca gtcggcagga actggcggac aacggcccgc
     2881 tgcggcggtt gtaccgccgc tgtgaccatt ggctcgcatc cgcccgcgtg ctgatcggcc
     2941 agccgggggg acgagggctg acggagtacc gcacggccgg cgcgctcaag gcggcgctgc
     3001 tgctggagca gtggcggcgc atgacgccgg tcagggagct ggaggaggaa ttccgggtcc
     3061 acgcggggca gatcatggcg atcggcgata cagccgcgca cctgctgagc ggcattgccg
     3121 ggttgctcga taatcttgac ggcggcggag acaaactccg cccgctcgtg ttcagcgttc
     3181 gcttcggagt cgtcccggag ttgcaggggc tgcacgcagc cttccgcgat atcctggcgc
     3241 gctccgattt tgcggcgctg gcgcaggcgg gaattgtccg ggtgcccgac ctgatcggac
     3301 acgacccgac cgacctgggg gagatcgtga agcaggagcg caaactgcag aaattgctgg
     3361 ccaggataat ggagtggaag aaggaggtaa gtatgcggca gtcggaggtt ctttcgcggg
     3421 tggcacccta tccggtgcgc gggtcggggc cgcggccggc gtcggtggag atcgacggat
     3481 catgtgagcg ggagcggtat ttggtgcggg tggacggccg gccggtgtgg ctgacgggga
     3541 aatcgttcaa gtacttcacg cggctggcct ggtcgcgtgt gcagcgggac ggggggtgga
     3601 tgtacaaaga ggatattgag cagggattca accaggcgcg ctacctgtac cggatgaaga
     3661 acgagatcaa cgaacgtctc gattcggaat gggaggtgtt cgagaacaac cgtctgggct
     3721 actatcggtt gaacgccgat gcggggagga tccggctgaa tgttgagcgg ctgcggaacc
     3781 accccgactg ggaggtgcgg tcgctgtttg cggggcagac cgagtcggcg gtcaactgag
     3841 accgggcggc gggaccggcc gggacgcgga caagacaatt gacgtatccc tcgcggcgcc
     3901 cggatccccc tcgtccgggg cggtgggagc gttcgccggc ggggcaggat tttttcctgc
     3961 cccgcttttg cgcgttgggc gggggtgcgc ggcaggaggt gacagggggg cgcggcccgg
     4021 gaagccgccg gagccggccg gcggtacgta taaagtgcgg tggcgcaacg gaattatgct
     4081 ttgacaatcg ttcagcaacg tgcctgattt gtcgaaaact agttgaagcg aggaagccgc
     4141 cgatgttcct cgccccatcg ctatgatagt acgcagcaaa aacaagctcc ccgtgctcgt
     4201 taccgaccag gagcagcagt acccggtgtt gtcgctgctc accggcgtgt tgttcccggg
     4261 ctcgatgatc accatccagg tcgggcggcg gtccagcatc gacctgattc gacagtgcca
     4321 cgagaggaag caggagtttg tggcgtcgtt cacgctgtcc cgccgcgggg gcgacgacgt
     4381 cggggcgctg cacgaggtgg gggtgctggc cgcggtccgc gacatccaga agggcgtggg
     4441 gaattcgctg atcgtcacga tcgaggggct gcggcgggcg gtgctggggg aggtagtccg
     4501 gcaggatccc tacctcacgg ccaccgtgct gtttctcgag gacccggcgg gccccgacaa
     4561 ggacatcgcg gccaaggtca ggcaggtcat cgacgtg
//
LOCUS       DAOPOE010000160         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_20959_length_4500_cov_5.663217, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000160 DAOPOE010000000
VERSION     DAOPOE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20959_length_4500_cov_5.663217"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKM94_13550"
     CDS             <1..752
                     /locus_tag="PKM94_13550"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD67702.1"
                     /translation="VVESDREAKEIIMRRAQIKREQEARRTHGRVTLDRVFDRIKEGQ
                     IKELRLIIKADVDGSVEVLSDTLGKIQSEEVKVNVIRRGVGGITESDVLLAAASDAII
                     IGFQVSPDSRAREVAKQESVDIRLYDIIYEAEQDVKKALEGLLAPTVREEFVGLCEVR
                     NTFRVPKVGMIAGCYVKEGRVNRKDSIRLIRDGKVIYRGQIASLKRFKDDAREVKEGF
                     ECGIGIERYDDVKVGDQIECLEVVEEARTLS"
     gene            762..1043
                     /locus_tag="PKM94_13555"
     CDS             762..1043
                     /locus_tag="PKM94_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOD67703.1"
                     /translation="MVTLVVKMRLDIPAIGSLKEKRRILKSMITRLRNDFNISIAEVD
                     LQDQIRAAVLGAAVISNSSAFCHQVMAKVIARVESSHEVILADYLTETY"
     gene            1043..1405
                     /gene="rbfA"
                     /locus_tag="PKM94_13560"
     CDS             1043..1405
                     /gene="rbfA"
                     /locus_tag="PKM94_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOD67704.1"
                     /translation="MADNRRQSRMGEQILRDISTMLEEELAEKVPGMVTFTRVRLTED
                     LRYATVYYSVLGKDGDREKVADYLEREKRRIRKMVGAGLRMRFIPEFAFKFDPSIEES
                     IRIQQLLDEIKSDRPPDE"
     gene            1371..2411
                     /locus_tag="PKM94_13565"
     CDS             1371..2411
                     /locus_tag="PKM94_13565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOD67705.1"
                     /translation="MKSRAIDRQMNDRPGPTAPPLDELRALLERSRRVLVVAHVEPDG
                     DAIGSLLACGACLRNLGKETVLVRESEIPSKYRFLRGAGEIRPVDALPVDLAIDTAVV
                     LECPTLDRVGRAARFLTDGVRIINIDHHRESGDFGAVNWIDPTKSSVGEMLFELYEAF
                     GCEITPEVAEQLYTAIMTDTGRFRYPSTSPRTMEIVGRLIAAGADPKAIADNVYYRLS
                     VSTMRLTGMVLASIEFHDRDRLCLLTLTQEMLEAAGAGLSESDGLVDFTLFTDTAEVG
                     ALLKEVDEDRTRVSLRSRDAVNVAALAAQYGGGGHFNAAGCTMPYGLARAREEIIRVL
                     THARKRDTDRQD"
     gene            2416..3336
                     /gene="truB"
                     /locus_tag="PKM94_13570"
     CDS             2416..3336
                     /gene="truB"
                     /locus_tag="PKM94_13570"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOD67706.1"
                     /translation="MLLIDKPLHCSSHDVVQTLRRITRQRRIGHAGTLDPLAEGLLVV
                     CLGHATKIARFLTGFDKVYVATICLGRTSATFDGEGIDAEAPAAPVPDFPRADLESAL
                     RRFEGRVTQRVPAFSAVRVEGVPLYKKARRGEEVDPPSREVTIREIRLLEVALPYLRI
                     EVTCSSGTYIRTLADDLGRVLGCGAYLAHLRRTRVGDLALSGALTLDQAADAMADGYL
                     HQHLLGYGEVLPYSALRVSEQFRPHVIEGRRPDRDDLTGLDGSFRAGDTVLLKSDDGA
                     VLAVGRAGVDSADLARPAARSLFDYIRVLH"
     gene            3333..4316
                     /locus_tag="PKM94_13575"
     CDS             3333..4316
                     /locus_tag="PKM94_13575"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOD67707.1"
                     /translation="MTTTFIRGIENFARAADGRPVIATIGTFDGIHLGHREIFRRVRA
                     QGERDNARAVLITFHPHPRVVVTPQNVPLLLTTIEEKEKFVPDFFEGNVLVLEFNEHL
                     KNMPADRFVRDILIDRIGVRKLIVGYDHAFGRNREGDIPFLQRIGAKLGFEVEVVAPV
                     VVDGQPVSSSRIRRALQENRFGEAIRLLGHEYAIYGTVERGIGLGKKLGYPTANVAYN
                     PRKLLPHDGVYACYAHVGSEEFDGMMFIGRNHFNPENRITVEANLFDFDRDIYGHEII
                     VYPMRHVRENRRFESREALVAQIGKDKEVILDIVKKEKRYAAQQGAQSQNH"
     gene            4279..>4500
                     /gene="rpsO"
                     /locus_tag="PKM94_13580"
     CDS             4279..>4500
                     /gene="rpsO"
                     /locus_tag="PKM94_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOD67708.1"
                     /translation="MPLNKEHKAKIIEENRLHGTDTGSPEVQIALLTARINHLTEHLK
                     SHSKDFHTRLGLLKLVGQRRRLLDYLMDRD"
BASE COUNT          812 a         1550 c         1392 g          746 t
ORIGIN      
        1 tggtcgtcga gagcgaccgc gaggccaagg agatcatcat gcggcgcgcc cagatcaagc
       61 gcgagcagga ggcccgccgc acccatggcc gcgtcaccct cgaccgcgtc ttcgaccgca
      121 tcaaggaggg ccagatcaag gagctccgcc tcatcatcaa ggccgatgtc gacggctcgg
      181 tcgaggtgct ctccgacacc ctcggcaaga tccagagcga ggaagtcaag gtcaacgtca
      241 tccgccgcgg cgtcggcggc atcacggaat ccgacgttct cctggcggct gcctccgacg
      301 ccatcatcat cggcttccag gtctcgcccg actcccgcgc ccgcgaagtc gccaaacagg
      361 aatcggtcga catccgcctc tacgacatca tttacgaggc cgagcaggac gtgaagaagg
      421 cgctcgaagg gctcctcgct ccgaccgtcc gcgaggaatt cgtcggcctg tgcgaggtcc
      481 gcaacacctt ccgggtgccc aaggtcggca tgatcgccgg ttgctacgtc aaggagggcc
      541 gggtcaaccg caaggacagc attcggctga tccgcgacgg caaggtgatc taccgcggcc
      601 agatcgcctc gctgaaacgg ttcaaggacg acgcccgcga ggtcaaggag ggtttcgagt
      661 gcggcatcgg gatcgagcgg tacgacgacg tcaaagtcgg cgaccagatc gaatgtctcg
      721 aagttgtgga ggaggcccgc acgctctcgt gaggcgcgcc cgtggttacg ctcgtggtca
      781 aaatgcgtct ggacatcccg gctatcggct cgctcaagga gaagcgccgg attctgaagt
      841 cgatgattac gcggctccgc aatgacttca acatctcgat tgccgaggtc gacctccagg
      901 atcagatccg cgccgccgtc ctcggcgccg ccgtcatctc caacagctcc gccttctgcc
      961 accaggtcat ggccaaggtc atcgcgcgtg tcgagtcctc ccacgaggtg atcctggccg
     1021 actacctcac ggagacctac tgatggcgga caaccggcgg cagtcccgca tgggcgagca
     1081 gatcctccgc gacatctcga ctatgctcga ggaggagctg gcggagaagg ttcccggcat
     1141 ggtcaccttc acgcgtgtcc gcctcaccga ggacctccgc tacgcgacgg tgtactactc
     1201 ggtgctgggc aaggacgggg atcgggaaaa ggtcgcggac tacctggagc gggagaagcg
     1261 gcgtatccgc aagatggtcg gcgccggcct gcgcatgcgc ttcattcccg agtttgcttt
     1321 caagtttgat ccgtccatag aagagagtat tcgcattcag cagctgctcg atgaaatcaa
     1381 gagcgatcga ccgccagatg aatgaccggc cgggcccgac agcgccgccg cttgacgaac
     1441 tccgcgccct tctggagcgg agccggcgcg tcctggttgt cgcccacgtt gaacccgacg
     1501 gcgacgccat cggctcgctg ctggcctgcg gggcctgcct ccggaacctc ggcaaggaga
     1561 cggtgctcgt ccgggagtct gaaatccctt cgaaataccg cttcctccgc ggcgccggcg
     1621 agatccgccc ggtcgacgcc ctccctgtcg acctcgccat cgacaccgcc gtcgtcctcg
     1681 aatgcccgac cctcgaccgc gtcggccggg ccgcccgctt tctcacggac ggtgtccgga
     1741 tcatcaacat cgaccaccac cgggagtcgg gggacttcgg cgccgtcaac tggatcgacc
     1801 cgaccaagtc ctcggtgggg gagatgctct tcgagttgta cgaggctttc ggctgcgaga
     1861 tcacgcccga ggtcgccgag cagctctaca ccgcgatcat gaccgacacc gggcgcttcc
     1921 gctacccctc gacgtcgccg cgcacgatgg agatcgtcgg gcggctcatc gccgccggcg
     1981 ccgatcccaa ggcgatcgcc gacaacgtct actaccgcct gagcgtctcc accatgcgtc
     2041 tgaccggcat ggtgctcgcc tcgatcgaat tccacgaccg cgaccgcctc tgcctgctca
     2101 ctctcacgca ggagatgctc gaggccgctg gcgccggcct gtccgagtcc gacggcctgg
     2161 tcgattttac gctgttcacc gacacggccg aggtgggcgc cctgctgaaa gaggtcgatg
     2221 aggaccgcac ccgcgtgtcg ctgcggtccc gcgacgccgt caacgtggcc gccctcgccg
     2281 cccagtacgg cggcggggga cacttcaacg ccgccggctg caccatgcct tacggcctgg
     2341 cccgcgcccg cgaagaaatc atacgcgtgc tgacccatgc ccggaaaaga gacaccgaca
     2401 gacaggatta acggcgtgct gctgattgac aaaccgctgc actgctccag ccacgacgtc
     2461 gtgcagacgc tgcgccgcat cacgcgccag cgccgcatcg gccacgccgg cacgctcgac
     2521 ccgctggccg agggactgct cgtcgtctgc ctcgggcacg ccaccaagat tgcccgcttc
     2581 ctgaccgggt tcgacaaagt ctatgtcgcc acgatctgcc tcgggcgcac ctcggccacg
     2641 ttcgatgggg aagggatcga tgcggaggcg ccggccgccc cggtgccgga tttcccgcgc
     2701 gccgacctgg aatccgcgct ccgccgcttc gagggccgtg tcacccagcg cgtcccggcc
     2761 ttctcggccg tgcgcgtcga gggcgtgccg ctgtacaaga aagcccggcg cggggaagag
     2821 gttgaccccc ccagccggga ggtgacgatt cgcgaaatcc gcctcctcga agtcgctctg
     2881 ccctacctgc gcatcgaggt gacctgctcc tcggggacgt acatccgaac gctcgccgac
     2941 gatctcggcc gggtgctcgg ctgcggagct tacctggcgc acctgcggcg cacccgggtc
     3001 ggcgacctgg cgctctccgg cgcgctgacg ctggaccagg ccgccgacgc gatggctgac
     3061 ggctatctcc accagcacct gctcgggtac ggcgaggtgc tgccgtattc ggccctccgg
     3121 gtcagcgagc agtttcgccc ccatgtcatc gagggccgcc gcccggaccg cgacgacctc
     3181 accggtctgg acggctcctt ccgcgccggt gacacggtgc tgttgaaaag cgatgacggc
     3241 gcggttctgg ccgtcggacg ggccggcgtc gattcggccg acctggcccg accggccgcc
     3301 cgctccctct ttgactacat acgggtgctc cattgacgac gacgtttatc cgagggatcg
     3361 agaatttcgc ccgggctgcc gacggccgcc cggtgatcgc caccatcggc acgttcgacg
     3421 gtatccacct gggccaccgg gagatcttcc gccgcgtccg cgcccagggc gagcgggaca
     3481 acgcccgcgc tgtgcttatc accttccacc cccacccccg ggtcgtcgtc accccccaga
     3541 acgtccccct cctcctgacc acgatcgagg agaaggagaa gttcgtcccc gatttcttcg
     3601 agggcaacgt cctggtgctg gaattcaacg aacacctgaa aaacatgccc gccgaccgct
     3661 ttgtccgcga tatcctcatt gacagaatcg gtgtgaggaa gcttattgtc ggctacgatc
     3721 acgcctttgg caggaaccgc gaaggcgaca tcccgtttct gcagagaatc ggcgcgaagc
     3781 tcggattcga ggtggaggtg gtcgcgccgg tggttgttga cggccagccg gtgtcgtcct
     3841 cgcgcatccg ccgcgcgctg caggagaacc gcttcgggga ggctatccgc ctcctcggcc
     3901 atgagtacgc catttacggc acggtcgaac ggggtatcgg gctgggcaag aagctcggct
     3961 atccgaccgc gaatgtcgcg tacaatccgc ggaagctgct gccgcacgac ggcgtttacg
     4021 cctgctacgc gcacgtcggc agcgaggagt tcgatggcat gatgtttatc ggccgcaacc
     4081 acttcaaccc ggaaaaccgg atcacggtgg aggccaacct gtttgatttc gaccgcgaca
     4141 tctatgggca cgagatcatc gtctacccga tgcgccatgt ccgcgaaaac cggcggtttg
     4201 agtccaggga agcgctcgtc gcccagatcg gcaaagacaa agaagtgatt ttagacatag
     4261 tgaagaagga gaaacggtat gccgctcaac aaggagcaca aagccaaaat cattgaggaa
     4321 aaccgcctgc acggcacgga cacgggttcg cccgaggtcc agatcgccct gctgacggcc
     4381 cgcatcaacc atctcacgga acacctgaag tcccacagca aggacttcca tacccgcctc
     4441 gggctgctga aactggtcgg ccaacgccgc cggctgctcg attacctgat ggaccgcgac
//
LOCUS       DAOPOE010000161         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_21301_length_4447_cov_5.709927, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000161 DAOPOE010000000
VERSION     DAOPOE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21301_length_4447_cov_5.709927"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKM94_13585"
     CDS             complement(<1..416)
                     /locus_tag="PKM94_13585"
                     /inference="COORDINATES: protein motif:HMM:NF023325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HOD67709.1"
                     /translation="MRVRQFFVLPKLPERLLPLQELAQNLWYAWSWDLVKLFIRLDAE
                     MWEQCYQNPVEMLSRLPQDALQRAADDDAFVAALHRVYDKYQDYLKAKKWFHYKYGQY
                     EGERIAYFSLEYGLDTSLPVYSGGLGVLSGDTLKSAS"
     gene            680..1834
                     /locus_tag="PKM94_13590"
     CDS             680..1834
                     /locus_tag="PKM94_13590"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOD67710.1"
                     /translation="MAKSVKRIGVLTGGGDCPGLNAVIRAIVKTADNEYDWNVIGFLD
                     GYEGLIDNRYRELAGSDVSGILTLGGTILGTSNQADPFHYPVLQDEGFIYLDRSSQAI
                     RNFEKLGLDTLVAIGGDGTMAASARMMEKGLPVVGVPKTIDNDLWGTDVTFGFDSAMA
                     TATEAVDRIHTTAQSHHRVMIIEVMGRYAGWLALGSGIAGGGDIILLPEFPYDIDSVC
                     GAIKARNAAGKRFSIVVVGEGAKPHGGDLVVERRIETSPDKIRLGGISRQVAAQIEGL
                     TNIECRVTILGHLLRGGSPTPRDRLLATRFGSEAVHLIARGETGRMVAMRSGDIGAVP
                     IVDVAGRLRVVTPDHPWVRAARSLGISLGIPPDARPEDFFAAPKQKAGKL"
     gene            1866..3251
                     /locus_tag="PKM94_13595"
     CDS             1866..3251
                     /locus_tag="PKM94_13595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD67711.1"
                     /translation="MITLSFHGAAGTVTGSKYLVTVNDAQVLIDCGMFQGPRDLRLKN
                     WEMPPFDPKALEAVIVTHAHIDHIGYLPRLGRMGFHKAIFATPPTVELARLALKDAAY
                     LQEEDAAYRNKKKLTRYEKALPLFDEDDVARIDEQYVDERFGKWVVAAEGIRFRYHIV
                     GHILGAACVEVAADDGGRAVSILFSGDVGRYGNPLTRNPGEPPVTDYLVCESTYGGRI
                     HEPEDPYFEFANLINEVVERRSILLVPAFAIGRTQQIVYLVNDLITHGRVPPIDIHID
                     SPMAVTATELYVKYADYHAIDLRRLAGEDCVLHGPRVFLHRERADSKELNKLRGPAII
                     LSASGMLTGGRILHHLIQRLPDPKNIVALVGYMAEGTLGRRLEEGAASVYIHKQPVEV
                     RAKVVKLHSLSAHADYHEMLHWLEPVSRRPRTVFCTHGEESQLAAMAGHLRDERGWEC
                     RIPRLHESVEL"
     gene            3271..4191
                     /locus_tag="PKM94_13600"
     CDS             3271..4191
                     /locus_tag="PKM94_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOD67712.1"
                     /translation="MARKPHAHDKAPRPRLTKTEMTRRLKRTPAYRLAYKDLDFLSTE
                     DCRPIRLELELLKPELLLRQNNIVSTIVVFGSTRIHDPAEARRAVVRLERLVRRAPHD
                     PQLRRRLRIARSVEAKSHYYEAAREFGRIVSEESQGNTENPYVIVTGGGPGIMEAANR
                     GAHDVEAKSIGLNITLATEQEPNPYITPELCMQFHYFAVRKMHFMLRARALVAFPGGY
                     GTLDELFEALTLVQTGVMKPLPIILFGEDYWRGLINFDYLVDQGVIDQDDLNLFVFAD
                     TARDAWEYIKYFYAEENNNHGIRDRRRPRP"
     gene            complement(4246..>4447)
                     /locus_tag="PKM94_13605"
     CDS             complement(4246..>4447)
                     /locus_tag="PKM94_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67713.1"
                     /translation="AVAEYDDTLDRLGYLRARPVEPERYPGFSGRYTWSEHTARRLAS
                     EDPQVRRPHLEAFLRERGLLRS"
BASE COUNT          777 a         1565 c         1387 g          718 t
ORIGIN      
        1 gaggccgatt tcagcgtgtc ccccgagagc accccgaggc cgccggagta cacgggcaga
       61 ctcgtatcca ggccgtactc gagggagaag taggcgatgc gctccccctc gtactggccg
      121 tacttgtagt ggaaccactt tttggccttc aggtagtcct ggtacttgtc gtacacgcgg
      181 tggagggcgg cgacgaaagc gtcgtcatcg gcggcgcgct ggagggcgtc ctggggcaga
      241 cgcgacagca tctccaccgg gttctggtag cactgctccc acatctcggc gtccagccgg
      301 atgaacagct tcaccaggtc ccagctccag gcgtaccaca gattctgcgc caattcctgc
      361 aggggcagga ggcgctccgg caacttcggg aggacaaaga attgacgtac tcgcatcgct
      421 ctcctacgct tccgcgggtc ggacggtccc ggggctagat aattgttcgg cagcggcgaa
      481 tgcaaccata aacttcccgg ctcccggacc gcgcggggtc ctctggccgg gttgtcggtc
      541 ctcctcatgt gatcggctcg tcgggcggtt tggttcactc cccgccgcga caatccccgg
      601 ccggtccctc aagctctcgg ccggccgggc cgacaaatat ttaggttgtt tttaaccccc
      661 tcccggcctt gtttacagca tggcaaaatc ggtgaaacga atcggtgtgc taaccggcgg
      721 gggcgactgc cccggcctca acgcggtcat tcgggcgatc gtcaagaccg ccgataacga
      781 gtatgactgg aacgtgatcg ggttcctcga cggctacgag ggactcatcg acaaccggta
      841 ccgggagctg gccggcagcg acgtctccgg gatcctaacc ctcggcggca ccatcctcgg
      901 aacctccaac caggccgacc ccttccacta cccggtcctc caggacgagg ggttcatcta
      961 cctggaccgg tcgagccagg cgatccggaa ttttgagaaa ctggggctcg acaccctggt
     1021 ggcgatcggc ggcgacggca ccatggccgc ttccgcccgc atgatggaga agggccttcc
     1081 cgtcgtgggc gtccccaaga ccatcgacaa cgacctctgg ggcaccgacg tcaccttcgg
     1141 cttcgattcc gccatggcca ctgcaaccga agcggtcgac cggatccaca cgaccgccca
     1201 atcccaccac cgggtgatga tcatcgaggt gatggggcgg tacgcggggt ggctggccct
     1261 cggctccggc atcgccggcg gaggcgacat cattctcctg cctgaattcc cctacgatat
     1321 cgacagcgtc tgcggcgcca tcaaggcccg gaacgcggcc ggcaagcggt tctcgatcgt
     1381 ggtggtgggg gagggggcga agcctcacgg cggggacctg gtggtcgagc ggcgcatcga
     1441 gacctcgcct gacaagatcc gtctgggcgg gatctcgcgc caggtcgccg cccagatcga
     1501 ggggctcacc aacatcgagt gccgcgtgac catcctcggc cacctgctcc ggggcgggtc
     1561 gccgacgccg cgcgaccggc tgctcgccac ccgcttcggc tcggaggcgg tccacctcat
     1621 cgcccgcggc gagaccgggc ggatggtcgc catgcgcagc ggcgacatcg gcgccgtgcc
     1681 gattgtcgac gtcgccggac gcctgcgcgt cgtaaccccc gaccacccct gggtccgggc
     1741 cgcccgctcg ttgggcatct cgctcggcat tccccccgac gcccgcccgg aagacttctt
     1801 cgccgcgccc aagcagaagg ccggaaaact gtagcggaca ccaccgaggg aaggcaggca
     1861 ttgtcttgat cacgctctcc tttcacggcg ccgccggcac cgtgaccggt tccaagtatc
     1921 tggtgactgt caacgacgcc caggtgctca tcgactgcgg catgtttcag ggcccgcgcg
     1981 acctgcggct gaagaactgg gagatgcccc ccttcgaccc caaagccctc gaggcggtca
     2041 tcgtcaccca cgcccacatc gaccacatcg gctacctccc gcggctcggg cgcatgggat
     2101 tccacaaggc gatcttcgcc acgccgccca ccgtcgaact ggcccgcctt gcgctcaagg
     2161 acgccgccta cctccaggag gaagacgcgg cttaccgcaa taagaagaaa ctgacgcgtt
     2221 acgagaaggc cctgccgctg ttcgacgaag acgacgtggc gcggatcgac gaacagtatg
     2281 tggacgagcg gttcgggaag tgggtggtgg cggccgaggg tatccgcttc cgctaccaca
     2341 ttgtcgggca catcctcggc gcggcctgtg tcgaggtggc ggccgacgac ggcggacgcg
     2401 cggtgtccat cctgttttcc ggcgacgttg ggcggtacgg caacccgctc actcgcaatc
     2461 cgggcgagcc gcccgtgacc gactacctcg tctgcgaatc gacttacggc gggcggattc
     2521 acgaacccga ggatccctat ttcgagttcg ccaacctgat caacgaggtg gtggagcggc
     2581 gctccatcct gctcgtcccc gccttcgcca tcggccgcac ccagcagatc gtctacctgg
     2641 tgaacgacct catcacccac ggccgcgtgc cgccgatcga catccacatc gactccccca
     2701 tggccgtcac cgccaccgag ctctacgtca agtacgccga ctaccacgcg atcgacctcc
     2761 ggcgcctggc gggggaggac tgcgtgctcc acggcccccg cgtttttctc caccgcgagc
     2821 gagccgattc caaggagttg aacaagctgc gcggcccggc catcatcctc tccgccagcg
     2881 gcatgctcac cggcgggcgc attctccacc acctcatcca gcgcctcccc gatcccaaga
     2941 acatcgtcgc cctcgtcggc tacatggccg aggggacgct ggggcggcgg cttgaggagg
     3001 gcgcggccag cgtgtatatt cacaagcagc cggtggaggt gcgggcgaag gtggtcaaac
     3061 tgcacagcct ctccgcgcac gccgactacc acgagatgct gcactggctg gagccggtca
     3121 gccgccggcc gcggactgtc ttttgcaccc acggcgagga gtcgcagttg gcggcgatgg
     3181 ccgggcacct gagagacgaa cggggttggg agtgccgcat ccccaggctc cacgaaagcg
     3241 ttgaactgta acggcaggag aggatcgacc atggccagaa agccgcatgc ccacgacaag
     3301 gccccgcggc cgcgtctcac caagaccgag atgacccgac gtctgaaacg gacgcctgcg
     3361 taccgcctcg catacaagga tctcgatttc ctgagcaccg aggactgccg ccccatccgg
     3421 ctcgaactcg aactgctcaa gccggagctg ctcctgcgac agaacaacat cgtctccacc
     3481 atcgtcgtct tcgggagcac gcgcattcac gatccggcgg aggcccggcg ggcggtggtc
     3541 cggctggaac ggctggtccg gcgcgcgccg cacgaccctc aactccgccg ccgcctgcgc
     3601 atcgcccgtt cggtcgaggc caagtcgcac tactatgaag cggcccgcga attcggccgc
     3661 atcgtctcgg aggagagcca ggggaatacc gagaatccct acgtcatcgt caccggcggc
     3721 ggccccggga tcatggaggc ggccaaccgg ggcgcgcacg atgtcgaggc caagagcatc
     3781 ggcctcaaca tcaccctggc caccgagcag gagcccaatc cgtatatcac gccggagctg
     3841 tgcatgcagt tccactattt cgcggtccgc aagatgcact tcatgctccg cgcccgcgcc
     3901 ctcgtcgcct ttcccggcgg ctacggcacg ctcgacgagc tcttcgaagc cctcacgctg
     3961 gtccagacgg gtgtgatgaa accgctgccg atcatcctgt tcggtgagga ctactggcgg
     4021 ggcctgatca atttcgacta cctcgttgac cagggggtca tcgaccagga tgatctcaac
     4081 ctcttcgtgt ttgccgacac cgcccgcgac gcctgggagt acatcaagta cttctacgcc
     4141 gaggaaaaca acaaccacgg catccgcgac cgccgccgtc cgcggccgtg acatccggcc
     4201 gggagccgca gcggccgtcc gccgacagcc cgccgaccga ccgctctacg agcgcagcag
     4261 tccccgttcc cgcaggaaag cctccaggtg cggccgccgc acctgcggat cctcgctcgc
     4321 cagccgccgg gcagtgtgtt ccgaccaggt gtagcggccg gagaatcccg ggtagcgctc
     4381 cggttcgacc ggtcgcgccc gcaggtagcc gaggcggtcg agcgtgtcgt cgtactccgc
     4441 cacggcg
//
LOCUS       DAOPOE010000162         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_22624_length_4249_cov_4.716023, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000162 DAOPOE010000000
VERSION     DAOPOE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22624_length_4249_cov_4.716023"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKM94_13610"
     CDS             complement(<1..616)
                     /locus_tag="PKM94_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOD67714.1"
                     /translation="MRRSFCLACAFLAAGAMGAGADNFQGSFDSPYTLPDSVVVTANR
                     FGSSDTRSAWPTEVITVDPAAAPLSLASGLDGAAGLDVRTQGGFGALATVSNWGAFNR
                     HLLLLYNGRPVKDYSLGGFNLAEFSPSELSRIEIVKGPQSAFYGSDAVGGVVNLIAPT
                     ALADRLEVHTTVGSFGARGVAARAARRLGALGIGAWTDYTAADNR"
     regulatory      complement(680..867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(917..1753)
                     /locus_tag="PKM94_13615"
     CDS             complement(917..1753)
                     /locus_tag="PKM94_13615"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HOD67715.1"
                     /translation="MRRLSLLFLAAAVPAAAAAASVVRFDPLQGLVEVEAVIDGHAKG
                     RFGIDTGADHFYIDSAFAAAHGLNLASGRPSRAVVGIDGHSTAIAGQVRSLEIGDERL
                     YNLDAIVMDIGALVKDDRADPPDGLIGYSLLRRFYVTVDYPNRRLILQQTEPDFLQSG
                     GLPSVPFETKGHLVVVQATFDDSATAPMALDYCASYVAVSPALAERLGVPAAAERTIL
                     GRLSLGGLITTDSVAVGIADISPIKARLRDAAFEGLIGASFLYRHKITIDYRRGRIYV
                     HR"
     gene            complement(1813..3033)
                     /locus_tag="PKM94_13620"
     CDS             complement(1813..3033)
                     /locus_tag="PKM94_13620"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOD67716.1"
                     /translation="MRTAVMCGLLGLVLAGCGGAPSPEALHRRALVADLHSDTPLRMR
                     DGFDIGAPHERGDGDMDLARLAEGGVDLQVFACWLPTELPTDSCVAAADLLIDSLLAQ
                     IARHRDRVGLCRTAAEAEEIIASGRLAALVSIENGVAIANDLANLGRFADRGVRCLAL
                     THTASNAWCISSADTAPAFVGLTDFGREVVREMNRLGMIIDISHAHPEAVAEVLRISA
                     DPVIASHSCVRALCDHDRNLTDDQIRAIAAAGGLIGVNFYNGYLSDEWNRVSDSLWRI
                     YRPTLDSLAALSRAEGADSQADSLRRAERRRIYREVAARVDSLAPVDVAAVCDHIDYI
                     VRLVGADYAGFGSDFDGVPSMPRGLEDCSGLPRITAELAARGYSESDIRKILGGNFLR
                     VFRRVCDRSPAASG"
     gene            complement(3069..3785)
                     /locus_tag="PKM94_13625"
     CDS             complement(3069..3785)
                     /locus_tag="PKM94_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD67717.1"
                     /translation="MPARRLIGIGACLLVAGAMVAPGAPPAMAQDQNFQAGLHFLAGL
                     PTGQFDDLVGDNAYGIGGHFLWSPPRSPLGLGLSLGFLNFGSESRREPFSRTIPDVRV
                     KVTTSNNMFQGLLFLRTQYRKGDFRPYADALAGFDYFWMETSVKDEDESEDIASSTDH
                     DDAAFTYGVGGGVMIRLHSGQSGGKPFALLLDLGARYLRGGEVEYARHKDIIEEQVTY
                     DIVTTESDLVVIALGVTGTF"
     gene            3982..>4249
                     /locus_tag="PKM94_13630"
     CDS             3982..>4249
                     /locus_tag="PKM94_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD67718.1"
                     /translation="MVYGVILAGGRGERFWPLSRIERPKQFLRLTSNKMMLEETIDRV
                     LPLIPRDRIRIVTGEEMRPYILQNCAAISDEHILAEPVGRNTAPA"
BASE COUNT          733 a         1401 c         1490 g          625 t
ORIGIN      
        1 ggcggttgtc ggcggcggtg tagtcggtcc aggcgccgat cccgagcgcg cccagccgcc
       61 gcgcggcacg ggccgcaacg ccgcgggcgc cgaacgaccc gacggtcgtg tgcacctcga
      121 ggcggtcggc gagcgcggtc ggcgcgatca ggttgaccac gccgccgacg gcatcggaac
      181 cgtagaacgc cgactgcggg cctttgacaa tttcgatgcg ggagagctcg ctcgggctga
      241 actcggcgag gttgaaccct ccgagcgagt agtctttgac cggccggccg ttgtacagca
      301 gcagcaggtg gcggttgaaa gcgccccagt tggagacggt cgcgagcgcc ccgaaccccc
      361 cctgggtgcg cacatccagc ccggcggcgc cgtcgagtcc cgaagccagc gaaaggggcg
      421 ccgcggccgg atcaacggtg atcacctcgg tcggccaggc cgaccgcgtg tccgaggaac
      481 cgaaccggtt ggcggtcacg accaccgaat cgggcagggt gtagggggaa tcgaatgatc
      541 cctgaaaatt gtcggccccg gctcccatcg cgccggcggc cagaaacgcg caggctaaac
      601 aaaaactgcg ccgcatggca acctccaccg gttgcggccg cgcagtgcct cgatctcgtc
      661 agcccgcgct gacagcaacc gcctgagatg tggtcgcggc aggtttcctg gcttcggcat
      721 ccaaccctcg ccgggcgcct tccccgcgaa tccgcgagtg gcctggtgcc cgggaagtag
      781 ccgttacagt agcggggctg cgacggactc gcaccgtctt ccctattacc cgtccctctg
      841 gacggcaccg caacgttgac gcgactatcg tagcgacggg aggggcggct gtcaagcgga
      901 aagcggagag gttggcctac cggtgcacgt agatgcggcc gcgccggtaa tcgattgtga
      961 tcttgtggcg gtagagaaag gacgccccga tgaggccctc aaaagcggcg tcgcgcaggc
     1021 gggctttgat cggggagata tcggcgatgc cgacagcgac gctgtcggtg gtgatgaggc
     1081 cgccgagcga caggcggccg agaatcgtcc gctcggccgc agcgggcacc ccgagccgtt
     1141 cggcgagcgc gggggagacg gccacgtacg aggcgcagta atccagcgcc atcggcgccg
     1201 tcgccgaatc gtcgaacgtc gcctggacca ccacgaggtg ccccttggtc tcgaacggca
     1261 ccgagggaag tccgccggac tggagaaaat ccggctcggt ctgctggaga atgaggcggc
     1321 ggttcgggta atcgaccgtg acgtagaagc ggcgcagcag gctgtacccg atgaggccgt
     1381 caggaggatc ggcccggtca tccttgacca gcgccccgat gtccatcacg atcgcatcga
     1441 ggttgtacag gcgttcgtcg ccgatctcca gcgagcgcac ctggccggcg atcgccgtgc
     1501 tgtggccgtc gatccccact accgcgcggc tggggcggcc ggaggccagg ttgagaccgt
     1561 gcgcggcggc gaaggcgctg tcgatgtaga aatgatcggc ccccgtatcg atgccgaacc
     1621 ggcctttcgc atggccgtcg atcaccgcct ccacctcgac cagcccctgc agcggatcga
     1681 agcggaccac gctcgccgcc gcggccgctg ccggcaccgc cgctgcgaga aagagcaggc
     1741 tcaggcgtct catatccgga agaaacaggg ccggggcggg ccggggcaag cgaaaacgcc
     1801 ggggaggggg gatcagccgg acgcggccgg cgaccggtcg cacacgcgcc ggaagacgcg
     1861 caggaaattg ccgccgagga ttttcctgat gtcgctctcc gagtagcccc gggcggcgag
     1921 ctcggccgtg atgcgcggaa gcccggagca gtcctcgagc ccccggggca tcgacggcac
     1981 gccgtcgaaa tcggagccga accccgcata atcggcgccg accagcctga cgatgtaatc
     2041 gatgtgatcg cacacggcgg ccacatcaac cggcgccagg gagtccacgc gggcggccac
     2101 ctcccggtag atgcggcgcc gctcggcgcg ccgcagactg tcggcctggc tgtccgcgcc
     2161 ctcggcgcgg gagagggccg ccagcgagtc gagcgtcggt ctgtaaatcc gccacaggga
     2221 atcggagact cggttccact cgtcggacag gtagccgttg tagaagttga cgccgatcag
     2281 tccgccggcg gccgcaatgg cgcggatctg gtcgtcggtc aggttgcggt cgtggtcgca
     2341 cagcgcccgt acgcacgagt gcgaggcgat caccggatcg gcgctgattc gaagcacctc
     2401 cgcgaccgcc tccgggtggg cgtgggagat gtcgatgatc atccccagcc ggttcatctc
     2461 ccgcaccacc tcccgcccga aatcggtcag tcccacgaag gccggtgcgg tgtcggccga
     2521 cgagatgcac caggcgttgg aggccgtgtg cgtgagcgcc aggcagcgca cgccgcggtc
     2581 ggcgaaacgg ccgaggttgg ccaggtcgtt ggcgatcgcc acgccgttct cgatgctcac
     2641 cagcgcggcc agccgcccgg aggcgatgat ctcctcggcc tcggcggcgg tccggcacag
     2701 ccccacccgg tcccggtgcc gggcgatctg ggccaggagc gagtcgatga gcaggtcggc
     2761 cgcggcgacg caactgtcgg tcggaagttc ggtcggcagc cagcaggcga acacctggag
     2821 gtcgacgccg ccctcggcga gccgggcgag atccatgtcg ccgtcgccgc gctcgtgggg
     2881 cgcgccgatg tcgaatccgt cgcgcatccg cagcggcgtg tcggaatgga ggtccgccac
     2941 cagcgcccgg cggtggaggg cctcgggcga cggcgcgccg ccgcaccccg cgaggacgag
     3001 tccgagcagt ccgcacatca cagccgtgcg catcgtgagt cctttccggt cggcgcgggc
     3061 cggccgcgtc agaatgtgcc ggtgaccccg agtgcgatca ccaccaggtc gctctcggtg
     3121 gtgacgatgt cgtaggtcac ctgttcttcg atgatgtcct tgtgccgcgc gtactcgact
     3181 tcgccgccgc gcaggtagcg cgcgccgagg tcgagcagga gggcgaacgg cttgccgccg
     3241 gactggcccg aatgcaggcg aatcataacg cccccgccga cgccgtaagt gaacgccgca
     3301 tcatcgtggt cggtcgagct cgcgatatcc tccgactcgt cttcgtcctt gacgctggtc
     3361 tccatccaga aataatcgaa tccggcgagc gcatcggcgt aggggcggaa gtcccctttc
     3421 cggtactgcg tgcgcaggaa caggagcccc tggaacatgt tgttgctcgt cgtgactttg
     3481 accctgacgt cggggatagt tcgggagaac ggttcgcgcc gcgactcgct gccgaaattg
     3541 agaaatccca gcgacagccc gagaccgagg ggggaccgcg gcggcgacca gaggaagtgg
     3601 ccgccgatcc cgtaggcgtt gtcgccgacg aggtcgtcga actggccggt cggcagcccg
     3661 gccaggaaat ggaggccggc ctggaagttc tgatcctggg ccatcgcggg cggggcgccg
     3721 ggagcgacca tcgcccccgc aaccagcaga cacgcgccga tcccaatcag ccgtcgtgcg
     3781 ggcatatcgt tttcctccaa caggtgtttg tgagtcggac gtttcgccca agaataggtc
     3841 ggaggggcgg ccaaggaaag agaaaacagg gcgcggtcgg gccgcgcgac ggggggcgac
     3901 cggcggcgga atctttcctt gactagtccg tttaaccctg atatcatcaa ccaattagct
     3961 cgaaagggaa agggtccgac cgtggtgtat ggggttattc tcgcgggcgg ccgcggcgaa
     4021 cgattctggc cgctctcgcg catcgaacgg ccgaaacagt ttctccggct gacctcgaat
     4081 aagatgatgc tcgaggaaac cattgaccgg gtgctcccgc ttattccgcg cgaccgcatc
     4141 cgcatcgtca ccggcgaaga gatgcggccg tacatcctgc aaaactgcgc cgccatctcg
     4201 gacgagcaca ttctggccga accggtcgga cgcaacacgg cgcccgcga
//
LOCUS       DAOPOE010000163         4219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_22795_length_4219_cov_6.122719, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000163 DAOPOE010000000
VERSION     DAOPOE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22795_length_4219_cov_6.122719"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKM94_13635"
     CDS             complement(<1..472)
                     /locus_tag="PKM94_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD67719.1"
                     /translation="MKTDKSKQIMERARRVIPGGVNSPVRAFKSVGGVPRVIARGEGA
                     YIWDADDNRYLDFGGSWGPLILGHADPEAVAAVVEQVRRGMTYGTATEIEFRLADFIV
                     RHIPAIEKIRFVSSGTEAVMSAIRVARGFTKRDLILKFDGCYHGHADPLLVQAGS"
     gene            complement(506..1423)
                     /gene="hemC"
                     /locus_tag="PKM94_13640"
     CDS             complement(506..1423)
                     /gene="hemC"
                     /locus_tag="PKM94_13640"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOD67720.1"
                     /translation="MTDARPLIIGSRGSDLALWQAHFISDILARELGQTVEIKIIKTA
                     GDRIQDLSFDKMEGKGFFTKEIEEALLAGDIDLAVHSLKDLMTTQPAGLKLAAAGYRA
                     DRRELLLIRPAAHHGDGLIPVREGSVIGTSSARRKCQIAFHRPSARIKDLRGNVPTRI
                     AKLRDGQYDAIVLALAGVERLRLDLSGLVALPLDPRQFLPAPAQGILGIQIRTHDPRV
                     EAAAGRLGSPEMMLTARVERGLLQRFGLGCSLPLGVYSERQGDAFRLLAVLGTQRDGA
                     WTGLKRAEAAGPANDPEQIVEAAYTKLKE"
     gene            complement(1420..2691)
                     /gene="hemA"
                     /locus_tag="PKM94_13645"
     CDS             complement(1420..2691)
                     /gene="hemA"
                     /locus_tag="PKM94_13645"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOD67721.1"
                     /translation="MELGIIGTSIWQQNMRLLETLTIDREGKLDRLVQLKAALGVDEL
                     IYLSTCNRVEFIFASRENGSTARVLHRLIDFFFAGGRDISFFPNDFYQYSGRDAVRHL
                     FRTVASLDSLVVGETQITGQFKEAAREATEAGLSGTMLDVLTKEALAVAKRVRTETSL
                     GDGAVSMASLAYSELEAALGDRRERPTVALVGAGAMTSKMARYIAKAGLAELVFVNRT
                     ADKAASLAREFGGRGMGLAEFLTTPPPVDAILSATASPAFVFTHEHLEKLSAGRPPVL
                     CIDLAIPQDFSPEFGRDPRAVLVDIPALKTREQRNLRRKFVESSRANEIVGEAVQAFL
                     RNRLEVSMKPILRDSYEQSLELANRALDDFFEKKLCELSREQKEHLRTLVIKLIGHSS
                     FGPARLLSNHLVDLQDQFLFDMLGESRKEAV"
     gene            2938..3258
                     /locus_tag="PKM94_13650"
     CDS             2938..3258
                     /locus_tag="PKM94_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD67722.1"
                     /translation="MDSATRQLFASRARVIKALAHPTRLFIVDELSRGERCVCALRDL
                     VGADISTVSKHLAVLKAAGIVDDDKRGLQVWYRLRVPCILNFFGCVEEVLRTHRRGVA
                     SGHE"
     gene            3336..>4219
                     /locus_tag="PKM94_13655"
     CDS             3336..>4219
                     /locus_tag="PKM94_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOD67723.1"
                     /translation="MDWRREWRPLAVLTAVFLGLYFLPLTGPRAASAWSEALALTQWY
                     AREHVILCLLPAFFIAGAIGVFVGKASVMKYLGAGADQRLAYGVASVSGSVLAVCSCT
                     VLPLFAGIYRMGAGLGPAAAFLYAGPAINVLAVILTARVLGLSLGVARAVGAVLFSIV
                     IGLLMHFFFRREAREREAAAAVLPADEPVRPLGQTALFFGLMIAVLVFATWGRPAQPV
                     GFWSAVYAIKWWLTGAAALALGALLWRRFGLAWWKVAAPAAVTLALAWLFPDRPQLVF
                     AVGVIGLSVVLAASPGEP"
BASE COUNT          672 a         1376 c         1359 g          812 t
ORIGIN      
        1 cgctgcccgc ctgcacgagg agcgggtccg cgtgcccgtg gtagcagccg tcgaacttga
       61 ggatgaggtc gcgcttggtg aatccccgtg cgacccggat cgccgacatg accgcctcgg
      121 tgcccgagga gacgaaacgg atcttctcga tcgccgggat gtgccgcacg atgaaatccg
      181 ccagtctgaa ttcgatctcg gtggcggttc cgtacgtcat cccccgccgg acctgctcaa
      241 ctaccgcagc gaccgcctcc gggtcggcgt ggccgagaat gagcggtccc caggatccgc
      301 cgaaatcgag gtaccggttg tcgtcggcgt cccatatata cgcgccttcc cctctggcga
      361 tgacgcgggg cacgccgccc accgacttga acgcccgcac cggcgaattg actccgccgg
      421 ggatcaccct ccgggcccgt tccatgatct gttttgattt gtccgtcttc atgtctatcc
      481 gatacgggat gtccgccgct cgcgctcact ccttcagctt cgtatatgcg gcctcgacaa
      541 tttgttccgg atcgttggcc ggtccggccg cctccgcccg tttcagaccg gtccaggcgc
      601 cgtcccgctg cgtcccgagc accgccagca gccgaaacgc gtctccctgc cgctcggagt
      661 acacgcccag cggcagcgag caccccagtc cgaaccgctg gagcaggccg cgttccaccc
      721 gggccgtgag catcatctcc ggagagccga gccggcccgc cgccgcttcc acccgcgggt
      781 cgtgcgtgcg gatctggatg ccgagaattc cttgggcggg tgcgggaagg aactgccggg
      841 gatccagggg cagcgcgacc aggccggaga gatcgaggcg gaggcgctcg accccggcga
      901 gcgcgagcac gatcgcatcg tactgcccgt cccgcagctt ggcgatccgg gtcggcacat
      961 ttccgcgaag atccttgatg cgcgccgagg ggcggtggaa cgcgatctgg cacttgcgcc
     1021 gggccgatga ggttccgatc acgctcccct cccgaaccgg gatgagaccg tcgccgtggt
     1081 gcgccgccgg gcggatcagg agaagttcgc ggcgatcggc gcggtacccg gcggcggcga
     1141 gcttgagccc cgcgggctgg gtcgtcatga ggtctttgag ggagtgcacg gccagatcga
     1201 tgtcgccggc caggagcgcc tcttcgatct ccttggtgaa aaaccccttc ccctccatct
     1261 tgtcaaagga caggtcctga atacggtcgc cggcggtttt gatgatcttg atctccaccg
     1321 tctgacccaa ctcgcgggcg aggatatcgg aaatgaaatg ggcctgccag agagccaggt
     1381 ccgagccgcg cgatcctatg atgagaggcc ttgcgtcggt caaaccgctt ccttacgact
     1441 ttcccccaac atatcaaaca ggaactgatc ctgcaagtcg acaagatggt ttgacaggag
     1501 acgcgcgggg ccgaaagacg agtggccgat gagttttatt acaagagtgc gaaggtgttc
     1561 tttctgttcg cgggagagtt cgcaaagctt cttctcaaag aaatcatcga gtgcccggtt
     1621 ggccagttcg agcgactgtt cgtagctgtc gcggagaatc ggcttcatcg atacctccag
     1681 ccgatttcgc agaaacgcct gcaccgcctc accgacaatc tcattggccc ggctcgactc
     1741 gacaaacttg cggcgcaggt tgcgctgctc gcgggtttta agcgcgggga tgtccaccag
     1801 caccgcgcgg ggatcgcggc cgaactcggg ggagaaatcc tgcgggatcg ccaggtcgat
     1861 acagagcacc ggcggccggc ccgcggagag cttctccagg tgctcgtggg tgaacacgaa
     1921 tgcgggggaa gcggtcgcgg agaggatcgc gtcgacgggc gggggagtgg tgaggaactc
     1981 cgccagtccc atcccccggc cgccgaactc gcgcgccaga ctcgccgcct tgtccgccgt
     2041 ccggttgaca aagaccagct ccgccaggcc ggccttggcg atgtaacggg ccatcttcga
     2101 tgtcatcgcg cccgccccga ccagcgccac ggtcggtcgc tccctccggt cgcccaacgc
     2161 cgcttccagt tcggagtacg ccagcgaagc catcgacacg gcgccgtcgc cgagactggt
     2221 ctcagtccgt acccgcttgg cgaccgcgag tgcctctttc gtcaacacgt cgagcatcgt
     2281 tcccgacaac cccgcctcgg tcgcttctcg ggcggcctct ttgaactgac cggtgatctg
     2341 ggtttctccc acgacgagcg agtcaaggga ggccacggtg cggaacaagt gacggaccgc
     2401 atcacggccg gagtactgat aaaagtcgtt gggaaaaaag gagatgtccc gaccgccggc
     2461 aaagaagaag tctatcaggc ggtgcaggac ccgggcggtc gagccgttct cacgcgaggc
     2521 aaaaatgaac tccacccggt tgcaggtcga caggtaaatc aattcgtcga ccccgagcgc
     2581 cgctttcagt tgcaccagac ggtcaagttt gccctcacgg tcgatcgtca acgtctccaa
     2641 cagccgcatg ttctgctgcc agattgaggt cccgatgatg ccgagttcca tgtccgtcct
     2701 taggcttgag ttccgccggc gtccatttcc attgtgggca aaggtatcat cccgccgaat
     2761 tgttttgtca tccgactgtc atagttgtga aaaaaggtac aagaagtggg aattaactct
     2821 ccacgagaac atactcaaga gtgtggattt tcccgcaccc gccgcccaaa aatcccttgc
     2881 ctcgcccaac tatttggcta ttttgccaaa taaaggaata ctcgtcaaag gcactgtgtg
     2941 gattctgcga ccagacaact tttcgcctct cgcgcccggg tcatcaaagc gctcgcccat
     3001 cccactcggc tgttcattgt cgatgagctc tcgcgcggcg aacggtgcgt gtgcgccctg
     3061 cgcgacctgg tcggggccga catctcgacc gtctccaagc acctcgccgt gctgaaagcg
     3121 gccggtattg tcgacgacga caagcggggt ctgcaggtgt ggtaccgcct gcgcgtccca
     3181 tgcatcctga actttttcgg gtgtgtcgag gaggttctcc gcacccatcg ccggggagtg
     3241 gcctccggcc atgagtgagc gtctctccgg gggtcgttcg gccggcgggt cggtcgccgg
     3301 gcggggatcc atcattgggg cgggtgggtg agccgatgga ctggaggcgc gaatggcggc
     3361 cgctggcggt tctcaccgcc gtcttcctcg gtctgtattt cctccccttg accggccccc
     3421 gggcggcctc ggcctggtct gaggcgcttg cgctcaccca gtggtatgcc cgcgagcatg
     3481 tgatcctgtg ccttctgccg gcgttcttta tcgccggggc gatcggcgtg ttcgtcggga
     3541 aagcatcggt gatgaaatac ctcggcgcag gggccgacca gcggctcgcc tacggcgtcg
     3601 cgtcggtttc gggttcggtg ctggcggtgt gctcgtgcac cgtgctgccg ctgttcgccg
     3661 ggatataccg catgggcgcc gggctcggtc cggccgccgc cttcctctac gccggccccg
     3721 cgattaacgt gctcgccgtc attctgaccg cgcgcgtgct cggcctgtcg ctgggggtcg
     3781 cgcgggcggt cggggcggtt ctgttcagca tcgtcatcgg cctgctcatg cacttcttct
     3841 tccgccgcga ggcgcgggag cgggaggccg cggccgccgt gttgccggcc gatgaaccag
     3901 tccgaccgct gggccagacg gcgctctttt tcggtctgat gatcgccgtg ctggtgttcg
     3961 ccacttgggg ccgcccggcc cagccggtcg ggttttggtc ggccgtgtac gcgatcaagt
     4021 ggtggctgac cggcgccgcc gcgctcgcgc tgggggcgct gttgtggcga cgattcggtc
     4081 ttgcctggtg gaaagtggcc gcgccggcgg ccgtgaccct cgcgctcgcc tggctcttcc
     4141 ccgatcggcc gcaactcgtg tttgccgtcg gcgtaatcgg gctctcggtc gtcctcgcgg
     4201 cgtcgccggg cgaaccgtg
//
LOCUS       DAOPOE010000164         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_23202_length_4162_cov_4.783540, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000164 DAOPOE010000000
VERSION     DAOPOE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23202_length_4162_cov_4.783540"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKM94_13660"
     CDS             complement(<1..190)
                     /locus_tag="PKM94_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67724.1"
                     /translation="MKAFSAWRRLYAPLIFCLALPAAGSAWAASGLAWRLETPPSWVS
                     TYQLPPDRKSVALADPIYP"
     gene            complement(258..1157)
                     /gene="murB"
                     /locus_tag="PKM94_13665"
     CDS             complement(258..1157)
                     /gene="murB"
                     /locus_tag="PKM94_13665"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOD67725.1"
                     /translation="MRSAFGPELEFDKPMAPLTSFRTGGTAAWFLPVRSAEAAAGAVQ
                     QARRMGLEFFLLGGGSNLLVADRGYGGLIIKMDVRGLARVDEATIECGAGEDLTALVT
                     YAQENGLAGLEFAAGIWGTVGGAIYGNAGAYGGEIGEVVTAVTLVDREGKVMTVDRAY
                     CGFGYRDSALKRSHEIVVSARFRLQPGDPAEIGRRVREILASRAQKHPDRLTAGCFFK
                     NIPDPREPYGKLPAGRLLEEAGAKGLAVGGARVFERHANIIVNAGGATAADIAALAAL
                     MKEKVRERFGIELQEEVIRLGEF"
     gene            complement(1359..1727)
                     /locus_tag="PKM94_13670"
     CDS             complement(1359..1727)
                     /locus_tag="PKM94_13670"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOD67726.1"
                     /translation="MAEDTTTPDPLFVQLVLSLQMGAWQQLGKIASPVSGQVDRDLAM
                     AKVSIDLLGMIQAKTAGNLNPEEKRMLDHVLYELRLNYVDELKKGETRPDAGTGSADD
                     AGRKPEDQPGGETAGSGKSD"
     gene            complement(1758..3845)
                     /gene="recG"
                     /locus_tag="PKM94_13675"
     CDS             complement(1758..3845)
                     /gene="recG"
                     /locus_tag="PKM94_13675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOD67727.1"
                     /translation="MAELKLDSPLQFVKGVGPRKAEVLAQHGLTTVRDLLSYLPRSYL
                     DRTSITAIKDVKVDQPATVVGQVKAHGMLHGKRRRYEVILQDETGALPLVFFQGLRYW
                     ERLFKKGQWFAATGTVGYFQGYQMVHPDLERLEDEGDQMIHAGRIVPVYPQTTELIRA
                     GLGSKGIRRLTTFVLANLAERIDDPVPSEETARCGLPARHEAIAKIHYPDRREQIEAC
                     RRRMAFDELLELQYLVLRWRQEKVKAVKEHRYAAPGEKLKKIGESLPFALTAGQKQVV
                     REIFADLQAPHPMARLLQGDVGCGKTVVAVLAALYAAENGLQTAFMAPTEILAAQHYG
                     NWREALESVGVRSALLTASLARAQKEAIAAACARGEIAILFGTHALIYDYVEFDRLGL
                     VIIDEQHRFGVRQRGRLAKKGDNPDLLVMTATPIPRTLALTLYGDLEISSITTMPPGR
                     KPVRTVWRHYTVRSRVYQFVREEIARGGQAYIIYPLVEKSDRLELENVEDAFRELSEK
                     EFRELQVGMVHGRIKAKERDEILRQFRDGRLHILMATTVVEVGIDNQRATLMLIEHAE
                     RFGLAQLHQLRGRIGRGAQTATLVALAHPPLSDMARRRLDYFAQTTDGFKIAEADLEL
                     RGPGEIFGLRQSGLPELRATRLTSDTDLLEAARMLVGRLLHEANRLDSRHQHLYTYLQ
                     ARTAGRELTPAGG"
     gene            4014..4110
                     /locus_tag="PKM94_13680"
     tRNA            4014..4110
                     /locus_tag="PKM94_13680"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4048..4050,aa:Sec,seq:tca)
BASE COUNT          669 a         1551 c         1208 g          734 t
ORIGIN      
        1 ggggatagat gggatcggcg agggcaacgg acttgcggtc cggcggcagt tggtaggtcg
       61 aaacccacga gggcggggtc tcgagccgcc acgccagtcc cgatgcggcc catgcggaac
      121 cggccgccgg cagcgcgagg cagaaaatca acggcgcgta cagacggcgc catgcagaga
      181 acgctttcac aaccactctg gtgccagaat gatacttcag tcgttagtca ctactcgata
      241 aatcgtcctc ctttccctca gaattcaccc agcctgatca cttcttcctg cagttcgatc
      301 ccgaaccgct cccggacctt ctccttcatg agcgccgcga gcgctgcaat gtcggccgcc
      361 gtggccccgc cggcgttcac gataatattc gcatggcgct cgaacacccg cgcccccccg
      421 accgccagcc ccttggcccc cgcttcttcg agcagccgcc ccgccgggag tttcccgtag
      481 ggctccctgg gatcgggaat attcttgaaa aaacaacccg ccgtcagacg gtcggggtgt
      541 ttctgcgccc gcgaggccaa aatctcgcgc accctccggc cgatctccgc cggatcgccg
      601 ggctgcagac ggaagcgcgc gctgacgaca atctcatgcg agcgcttgag cgccgagtcc
      661 cggtagccga acccgcaata ggcgcggtcc accgtcatga ccttcccctc gcggtccacc
      721 agcgtcacgg ccgtgacaac ttcgccgatc tcgccgccgt aggcgccggc gttgccgtag
      781 atggctcccc cgacggtgcc ccagatgccg gcggcgaatt cgagcccggc cagaccgttc
      841 tcctgcgcat acgtcacgag cgccgtcaaa tcctcccccg ccccgcactc gatcgtcgcc
      901 tcatcgaccc gcgccagccc tcgcacatcc attttaatga tcagaccgcc atagccccgg
      961 tccgccacca gcaggttaga tcctccgccc agcagaaaaa actccagacc catccggcgg
     1021 gcctgctgca ccgccccggc ggcggcttcc gccgagcgca ccggcaaaaa ccacgccgct
     1081 gtcccgcccg tgcggaaact cgtcaacggc gccattggtt tgtcaaattc gagttccggc
     1141 ccgaatgcgc tgcgcatctc ggccgtagac acctgcgccc gcggcaccgc tcgttgggtt
     1201 gatctcacca ccggaatata ctcgcaaaat ccttgaaaat caaggagaaa cagccacgtc
     1261 cggggtcgcg gattccgccc cgccgtctct ctccttatta tacaccctcg gcggggcgtt
     1321 gtctctgcgc cgcccccgcc ggaccacaat tccttgcctc aatccgactt cccggacccc
     1381 gccgtctccc cgcccggctg gtcctcgggc tttcggccgg catcatcggc gctcccggtt
     1441 cccgcgtccg gccgagtctc cccttttttc agctcatcga cataattcag ccgaagttca
     1501 tacaaaacgt gatcgagcat ccgcttctct tccggattca gattcccggc cgtcttggct
     1561 tgaatcatgc cgagcaggtc gatgctcact ttcgccatcg ccagatcccg gtcaacctgg
     1621 ccgctgaccg gcgaggcgat tttccccagt tgctgccaag cccccatctg gagcgacaag
     1681 accagttgga caaacagcgg gtcgggcgtg gtcgtgtctt cggccatagt cacctccggt
     1741 caggcccgcc gcctcggtta tcccccggcc ggcgtcagtt cccggccggc ggtgcgagcc
     1801 tgaaggtagg tatacagatg ttgatgccgg ctgtcaaggc ggtttgcctc gtggagcagc
     1861 cgcccgacca gcatgcgcgc cgcctcgaga aggtcggtgt ccgaggtaag gcgggtggcc
     1921 cgtaattccg gcagccccga ctggcgcagg ccgaaaatct cccccggacc ccgcaattcc
     1981 agatcggcct cagcgatctt gaatccgtcg gtcgtctgcg cgaaatagtc cagccggcgg
     2041 cgggccatgt ccgacagcgg cgggtgggcc agggccacca gcgtcgccgt ctgcgccccc
     2101 cgtccgatcc gcccccgcag ttggtggagt tgcgcgaggc caaaccgctc ggcgtgctcg
     2161 atcagcatga gcgtcgcccg ctggttgtcg atccccacct ccaccaccgt cgtcgccatc
     2221 aaaatgtgca gccgcccgtc ccgaaactgc ctgaggatct catcccgctc cttcgccttg
     2281 atccgcccgt gcaccatgcc cacctgcagc tcccggaact ccttctccga gagctcccgg
     2341 aaagcatcct ccacgttctc cagctccagc cggtcgctct tctccacgag cgggtagatg
     2401 atgtaggcct ggccgccgcg ggcgatctcc tcgcgcacaa actggtagac ccggctgcgc
     2461 accgtgtagt gccgccacac cgtccgcacc ggcttccgtc ccggcggcat ggtggtgatc
     2521 gacgatatct cgaggtcgcc gtagagcgtc agcgccagcg tgcgcgggat cggcgtcgcg
     2581 gtcatcacca ggagatccgg gttgtccccc ttcttggcca gccgcccccg ctgccggacg
     2641 ccgaaccggt gctgttcgtc gataatcacc agccccaggc ggtcgaactc cacgtagtcg
     2701 tagatcagcg cgtgcgtgcc gaagagaatg gcgatctctc cgcgcgcgca ggccgccgcg
     2761 atggcctcct tctgcgcccg cgccagactg gccgtcaaca atgccgaccg caccccgaca
     2821 ctctccagcg cctcccgcca gttcccatag tgctgggcgg cgagaatttc ggtcggcgcc
     2881 atgaacgccg tctgaaggcc gttctcggcg gcgtagaggg ccgccagcac cgccaccacc
     2941 gttttcccgc acccgacatc cccctgcaac agccgcgcca tcgggtgcgg cgcctgcagg
     3001 tccgcgaaga tttcccgcac cacctgcttc tgccccgccg tgagcgcaaa cggaagactc
     3061 tccccgatct tcttcagctt ctcccccggc gccgcgtacc ggtgctcctt gaccgccttc
     3121 accttctcct gtcgccaccg caggacgagg tactgcagct ccaggagttc gtcgaaagcc
     3181 atgcgccgcc ggcacgcttc gatctgctcg cggcggtccg gatagtggat cttggcgatc
     3241 gcctcatgcc gcgccggcag gccgcagcgc gccgtctcct cgctcggcac cggatcgtcg
     3301 atccgctcgg ccaggttggc gagcacgaaa gtggtcagcc gccgaatccc cttgctcccc
     3361 agccccgccc ggatcaattc ggtcgtctgc gggtacaccg ggacaatccg ccccgcgtgg
     3421 atcatctggt ccccctcgtc ctccagccgc tcgagatccg ggtgcaccat ctggtaaccc
     3481 tggaagtagc cgaccgtccc ggtggcggcg aaccactgcc ccttcttgaa gagccgctcc
     3541 cagtagcgca gcccctggaa aaacaccagc ggcagcgccc ccgtctcgtc ctgcaggatc
     3601 acctcgtagc gccggcgctt cccgtgcagc atcccgtgag ccttcacctg gccgaccacg
     3661 gtcgccggtt gatcgacctt gacgtctttg atcgccgtga tgctggtgcg gtcgaggtag
     3721 ctgcgcggca ggtaggacag gagatcccgc accgtggtca gaccgtgctg ggcgagcacc
     3781 tcggctttgc ggggtccgac gcccttgacg aattgcagcg gcgaatcgag tttgagctcg
     3841 gccatagggt aagagtggcg cgccggcgtc cccgagtcaa tcataattcg ctgcggggca
     3901 tccgcttctc ccggcgccgc gaaccgcggg cgggcgccgg cggcagcgcg cgccccccgg
     3961 ccgcgcccgg ctcgcccctt gttttttcac ccccgcaagc gtatcatgcc cccggaagcg
     4021 gatgctgtct ggtggcggtc ccggccttca aagccgtgtg gggggcagtc gtactgtccc
     4081 cggtgggttc gattcccacc ccttccgcca aatttcaggg aggtcggaag acaggccggc
     4141 atggcagtcg agaatccgga tc
//
LOCUS       DAOPOE010000165         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_23538_length_4117_cov_3.806745, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000165 DAOPOE010000000
VERSION     DAOPOE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_4117_cov_3.806745"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1297
                     /locus_tag="PKM94_13685"
     CDS             179..1297
                     /locus_tag="PKM94_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOD67728.1"
                     /translation="MALLLEVIEWMDVSGDEMIHRIPNEGSADFKLGAQLIVRDSQMA
                     IFFKDGHAADQFATGRHTLTTLNIPILTRLAAFPFGFNSPFRAEVYFVNLKVFTNLKW
                     GTKHPVTFRDKELGLIRLRGHGAFTMQIENPSLFLNSIVGRQALYGTGDIQDYLRDVI
                     IARMNDLLGEKLASVLDLPAVYTELAEEFKQIVKVEFEKYGLRLVDFFIASITPPDEV
                     SKMIDQRSGMEAVGNLDRFLKFEMARGLGSTGAAGAAGAGMGMAAGVGVMMPGMIQKV
                     FAPEQTELRREPVATVTCPKCHTDTPEQSRYCYRCGHQMVAQNSCPECGVQLPTEARF
                     CFACGKKLDAPAICPHCSAKLIPGSKFCGDCGKPVKED"
     assembly_gap    1412..1421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1422..2870
                     /locus_tag="PKM94_13690"
     CDS             <1422..2870
                     /locus_tag="PKM94_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67729.1"
                     /translation="QGFFIAVGCPGCGGELELGEDFWVLRCPYCASLLKVERPGGGAR
                     AGTAGAGDGSNDRPAAFVVAGRVQEYEVRFGLDRHLKARGEPLTGGGLRFKKIYYPYW
                     KIDAILLKTRNRVRVVEEVPEDGQPSSYESDRTVRRTEVSLSPYQITFPAGAPIAGVP
                     YSAGLRATYVKGAPFAEEEIQEGYRALPATVSWARARQTALRTVANVGRIETAEFGQN
                     RTEMFRPRGTIVYFPYWIVEETGGAGYRRWIVDGVTGQPAGETAPPLEGAPEAEAVSE
                     AAPLEFGPLVVGMHRCGNCGHGLPEEQSYLYVCRQCGVRTTVEEHPLFSGEVAAADQP
                     AGPEDVLLPFWLVTMTSELAGRLKILAGGMGQSGVLAVPAFRVDNFEAAYRLGRRMTT
                     AAGKFGGRDLGEETERLAPVGVGPAEALVLARAMAARELLGQSSTLALPEAGTGFTGL
                     RLLFVPFHREHYFFIDSITQAVAVEKRLIPVD"
     gene            3098..>4117
                     /locus_tag="PKM94_13695"
     CDS             3098..>4117
                     /locus_tag="PKM94_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67730.1"
                     /translation="MGKLYHLAGLTVVAALLAAATPSHGALRKTYELTGDPDTTAASN
                     TAIDIVAHAGGIWLGTPAGLNFSLDSGKTWLLYRKGNGLRQDDVSALFSSGDRLWVGT
                     AWTEAIAGVGTVTYSDTLQYTNDNGETFPTVDLKRPGATVPYAFGPAKTIFDLAVGRA
                     QGDEWVFMANFAGGLVGSRSGGEAWRRIFFSRSDSINFYQTYESTSPPPLAYRSRTFS
                     VAADTTHGDSMYVWAGTAQGVLQYVYAPAADKPNSNATTAMVLCPECVPGESTYVFVG
                     GDRGLTRGSIQGQPYRSMDTGDGLPGPYVTMLDHFAGRLFVGTRESRDGASTGLAYSD
                     DGGETF"
BASE COUNT          703 a         1122 c         1609 g          673 t
ORIGIN      
        1 ggctggattc ccgccttcgc gggaatgaca agggggagcg gcatcgacag gacggggagg
       61 aacgaataga cggggcgggg aaggacgagg gtccggcggg gggagggtca gttagtcgaa
      121 agatcggaga tgagagcaaa ggaaaacaag ggccaatagc gaagagactg gagtgccaat
      181 ggcgttacta cttgaggtga tcgagtggat ggatgtctcg ggggacgaga tgatccatcg
      241 aatccccaac gagggatcgg ccgatttcaa gctcggggcg cagctaatcg tgcgcgacag
      301 ccagatggcg attttcttca aggacgggca cgcagccgac cagttcgcca ccggccggca
      361 cacgctgacc acgcttaaca tcccgatcct cacgcggctg gcggcgtttc ccttcgggtt
      421 caactccccc ttccgggccg aagtgtattt cgtcaacctg aaagtgttca ccaacctgaa
      481 atgggggacg aaacacccgg tgacgttccg ggacaaggag ctggggctga tccggctccg
      541 cgggcacggg gccttcacaa tgcagatcga gaacccctcg ctgttcctca attcgatcgt
      601 cgggcggcag gcgctctacg ggacgggcga tatccaggac tacctccgcg acgtcatcat
      661 cgcgcgcatg aacgacctgc tcggggagaa gctcgccagc gtgctggatc tcccggcggt
      721 gtatacggag ctggcggagg agttcaagca gatcgtgaag gtcgagttcg agaagtacgg
      781 gcttcggctg gttgattttt tcatcgcctc gatcacgccg ccggacgagg tgtcgaagat
      841 gatcgaccag cggtcgggga tggaggcggt ggggaatctc gaccggttcc tgaaatttga
      901 gatggcgcgg gggctgggct cgaccggggc ggcgggagcg gccggggccg ggatggggat
      961 ggcggccggg gtgggggtca tgatgccggg gatgatacag aaggtgtttg cgccggagca
     1021 gacggaactg cggcgcgagc cggtggccac cgtgacctgt ccgaaatgcc acaccgacac
     1081 gccggagcag tcacggtact gctaccgctg cgggcaccag atggtcgcgc agaacagctg
     1141 ccccgaatgc ggcgtgcagc ttccgaccga ggcgaggttc tgtttcgcct gcggcaagaa
     1201 gctcgatgcg ccggcgatct gcccgcactg cagtgcgaag ctgatcccgg ggtcgaagtt
     1261 ctgcggcgac tgcggaaagc cggtgaagga agactagcgg gcggcccggc ggcgcggggt
     1321 cgggcgtctg cggacgcctg atccgccgcc ggtggaccgg caaccgacag gaccggaaag
     1381 atgagtgcga cagccaaaac agcggggcac annnnnnnnn ncaggggttc tttatcgcgg
     1441 tcgggtgtcc cgggtgcggc ggagaactcg agctgggcga ggatttctgg gtgctccggt
     1501 gcccttactg cgcatcgctc ctgaaggtcg agcggccggg gggaggcgcg cgggcgggga
     1561 cggcgggcgc cggagacggc tcgaacgatc ggccggcggc gttcgtagtc gcgggccggg
     1621 tgcaggagta cgaggtgcgg ttcggcctcg accggcacct caaggcgcgg ggcgagccgc
     1681 tcaccggcgg agggctgcgc ttcaagaaga tctactatcc ctactggaag atcgacgcca
     1741 tcctgctcaa gacgcgcaac cgggtccggg tggtcgagga ggtgccggag gacggacagc
     1801 cctcctcgta cgagtccgac cggacggttc ggcggacgga ggtatcgctc tccccctacc
     1861 agatcacgtt tccggcgggt gcgccgatcg cgggggtgcc gtactcggcg gggttacggg
     1921 cgacctacgt caagggggcg ccgtttgcgg aggaggagat ccaggagggg taccgggcgc
     1981 tgccggcgac ggtgagctgg gcgcgggcgc ggcagacggc gctgcggacg gtcgccaacg
     2041 tcggccggat cgagaccgca gagttcgggc agaaccggac ggagatgttc cggccgcggg
     2101 ggacgatcgt gtatttcccg tactggatcg tggaggagac cggcggtgcg gggtatcggc
     2161 ggtggatcgt ggacggcgtg acgggacagc cggcggggga gacagcgccg ccgctcgagg
     2221 gagcaccgga ggccgaggcg gtttccgaag cggcgccgct ggagttcgga ccgctggtag
     2281 tggggatgca ccggtgcggc aactgcgggc acggcctccc ggaggagcag tcgtacctct
     2341 acgtctgccg gcagtgcggc gtgcggacga cagtggaaga gcacccgctg ttttcagggg
     2401 aggtggccgc ggccgaccag ccggccgggc cggaggatgt gctcctgccg ttctggctgg
     2461 tgacgatgac gtcggaactg gcggggaggc ttaagatcct tgcggggggg atggggcaat
     2521 cgggggtgct ggcggtgccg gcgtttcggg tggacaattt cgaagccgcc tatcggctgg
     2581 ggcggcggat gacgacggcg gcggggaagt tcggggggcg ggacctcggg gaagagacgg
     2641 agcggctggc gccggtcggt gtggggccgg cggaggcgct ggtgctggcg cgggcgatgg
     2701 cggcgcggga gcttctgggc cagtcgagca cgctggcgct gccggaggca ggtacggggt
     2761 ttacgggact tcggctgttg ttcgtgccgt ttcaccggga gcactatttc ttcatcgact
     2821 cgatcaccca ggcggtggcc gtggaaaagc gcctgattcc ggtcgactag ctggaactcg
     2881 ttggattcat tggggtttaa ggggagacag caggaggtac gccgaccgaa aaagattgct
     2941 attgaccgaa caatggtgta tgttcgagcc tgactgcgcg agaagaacgg gccaggctgt
     3001 ttggttggta taccttgctg aaatcacaga gtctcatcat cgccgtagaa gccgtgtcag
     3061 cggctggtcg cggctttttt gtttgaaggg gtgcgttttg ggcaaactct accatctggc
     3121 ggggctgacg gtggtcgcgg cgcttctggc ggcggccacg ccgtcacacg gggcgctccg
     3181 gaaaacatac gaactcaccg gcgatccgga cacgacagcg gcgagcaaca cggcgatcga
     3241 cattgtcgcg cacgccgggg ggatctggct ggggacgccg gcggggctga atttcagcct
     3301 cgacagcggc aagacgtggc ttttgtaccg caaggggaac gggctgcggc aggacgacgt
     3361 gtcggcgctg ttttcctcgg gcgaccggct gtgggtgggg acggcgtgga cagaggcgat
     3421 cgccggggtc gggacggtga cttattcgga cacgctgcag tacacgaacg acaacgggga
     3481 gacctttccc acggtcgacc tgaaacggcc gggggcgacg gtgccgtacg cgtttgggcc
     3541 ggcgaaaacg atatttgacc tggcggtcgg ccgggcgcag ggggacgagt gggtgttcat
     3601 ggcgaatttc gccggcgggc tggtggggtc gcggagcggc ggggaggcct ggcggcggat
     3661 cttcttctcc cggtcggatt cgatcaactt ctaccagacg tatgagtcaa catcgccgcc
     3721 gccgcttgcc taccggtccc gcacgttttc ggtggccgcg gacacgaccc acggcgattc
     3781 gatgtacgtg tgggcgggga cggcgcaggg ggtgctccag tacgtctacg ctccggccgc
     3841 cgacaagccg aactcgaatg cgacgacggc gatggtgctg tgcccggagt gcgtgccggg
     3901 ggagagcacc tatgtctttg tcggcgggga ccgggggctg acgcggggct cgatccaggg
     3961 gcagccgtac cggtcgatgg acacgggcga cgggctgccg gggccatacg tgacaatgct
     4021 cgatcatttc gcggggcggc tgttcgtggg aacgcgggag agccgggacg gggcttcgac
     4081 cgggctggcg tattccgacg atggtggcga gacgttt
//
LOCUS       DAOPOE010000166         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_23996_length_4054_cov_5.773693, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000166 DAOPOE010000000
VERSION     DAOPOE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23996_length_4054_cov_5.773693"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PKM94_13700"
     CDS             complement(<1..950)
                     /locus_tag="PKM94_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOD67731.1"
                     /translation="MSCATGTDDQKTIPTRDQVEERYQWNLADLYLSDEAWDDDARRV
                     EELIRNAAGFAGRLHESPDTLYRCLKSRSDLGLAMFRLYQYAKLSQDIDNRVSKYQAM
                     TDRAAALSARGRAAFAFVEPELLAIDEQALRAMAARFPHTDEYDFYINELIRTRAHVR
                     TQEVEELLALSAQVTRGPETIFTMLDDADLSYPTVQDESGRDVQLTKQRYAKLMESSD
                     RRVRHDAYHAFYRPYREHLNTLGASLAAAVNADVFYARARRFDSCLAAALFGDNIPVS
                     VYRNLIETTEAHLEGMHHYLRVRQRLLKVEQLHTWDLTCPL"
     gene            complement(1109..2785)
                     /locus_tag="PKM94_13705"
     CDS             complement(1109..2785)
                     /locus_tag="PKM94_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD67732.1"
                     /translation="MRKSFLLRTTAGVLVFAAALLLAATVPAADKTAELDAYLSAACD
                     VLRFQGAVLVADDGVPVFVKGYGMADVAHGQPNTPETEFLIGSVTKQFTAAAILQLQE
                     QGTLSVHDPISKFLPDFPKATGDRITVHHLLTHTSGLVSYTDIDSLMARRGLDMTARE
                     IVASFKDLPLQFEPGAMYRYCNSGYFLLGLIIEAVSGQTYADYLAEHIFRPLGMTHTT
                     YPPNERTPNRAVGYVAPEDTLVPADPISMTLPYAAGALASTVGDLLIWDQALYGDRVL
                     SEDSRKQMYTPALENYGYGWMVAERFGRREISHGGGIDGFTSVIARYPDDRLTIIVLC
                     NNMSANAAGLGSTLAAIMFDQPYDRPVRKTPAAADPASFDALVGVYRVGDNSYRVIRR
                     DGDNLYSQRTGGPVFQVFPEAPDKFFFENDNSTTLVFIRDQAGAVVAHLIHQSGVDTR
                     CDRVTGPLADSLMAERAVVPIDPAVFARLVGDYELAPGFVLTFRARDGRFFSQATGQQ
                     EFEIFPAGETEYFLRVVDAQITFQLDPATGRATGLILHQGGRDMPAKKIK"
     gene            complement(2900..3733)
                     /locus_tag="PKM94_13710"
     CDS             complement(2900..3733)
                     /locus_tag="PKM94_13710"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOD67733.1"
                     /translation="MQTIEEFRAMLNDAVGYITSQIAFTPRIGIILGTGLGKLVDGID
                     MVGTVSYEKIPHFPVSTVESHAGRLLFGRLRGKPVVCMQGRFHYYEGYSFPQIAFPVR
                     TLKALGIHTLIVSNAAGGLNPNFAPGDIMLIKDHINFFPGNPLIGPNDDTLGDRFPDM
                     YEVYHRRYQALARQVALELKIRLQEGVYVGLTGPCLETAAEYRMLRGFGADAVGMSTV
                     PEVLTAHHQRTKILAFSIITDMGLPDNMHPCSLADVIGAASATEPILRDLIAGCVEKM
                     E"
BASE COUNT          682 a         1140 c         1525 g          707 t
ORIGIN      
        1 agcgggcagg tgagatccca ggtgtggagc tgctccactt tgaggagacg ctgccggacg
       61 cgcaggtagt ggtgcatccc ctcgagatgg gcctcggtgg tctcgatgag gttgcggtag
      121 acggagacgg ggatgttgtc tccgaagagg gcggccgcca ggcagctgtc gaaccggcgg
      181 gcgcgggcgt agaacacgtc ggcgttgacc gcggcggcga gcgacgcccc gagcgtgttg
      241 aggtgctccc ggtaggggcg gtagaacgca tggtaggcat cgtgccgcac ccggcggtcg
      301 gacgattcca tcagcttggc gtagcgctgc ttggtgagct ggacatcccg gccggactcg
      361 tcctggacag tcgggtagga gaggtcggcg tcgtccagca tggtaaaaat tgtctccggg
      421 ccgcgagtga cctgggccga gagggcgagg agttcctcca cctcctgggt gcggacgtgg
      481 gcgcgggtgc ggatgagctc gttgatgtag aagtcgtatt cgtcggtgtg cgggaagcgc
      541 gcggccatcg cgcggagcgc ctgctcatcg atcgccagca gttcgggttc gacaaaggcg
      601 aaagcggcgc ggccgcgggc agaaagggcc gcggcgcggt cggtcatggc ctgatatttc
      661 gagacccggt tgtcgatatc ctggctcaac ttcgcatatt ggtagagacg gaacatggcc
      721 agcccgaggt cggagcggct cttcagacac cggtagagcg tgtccggcga ctcgtggagg
      781 cgcccggcga acccggcggc gttccggatg agttcctcga cgcggcgggc gtcgtcatcc
      841 caggcctcgt cggacagata gaggtcggcg aggttccatt ggtagcgttc ctcgacctgg
      901 tcgcgggtgg ggattgtttt ctggtcatcg gtgccggtcg cgcaggacat ggggggcctt
      961 tcgtttggtt ggttcgccat cgggcgcaat atcgcaggta agagcggcgg gcgcaagggc
     1021 ggcgggcggg attttgccgg ccgcgggcga gccgggctgc tcgtccgctt tcggcgaacg
     1081 cggaacccga cccacccgcg gcttagggtc acttgatctt cttggccggc atgtcgcgac
     1141 ctccctggtg gaggatcagg ccggtggcgc ggccggtcgc cgggtcgagc tggaatgtga
     1201 tctgcgcgtc gacaacacgc aggaagtatt cggtttcgcc ggcggggaag atttcgaatt
     1261 cctgctggcc ggtggcctgc gagaagaagc ggccgtcgcg ggcgcgaaac gtgaggacaa
     1321 accccggcgc gagttcgtag tcgccgacca gacgcgcgaa gaccgccggg tcgatcggga
     1381 ccaccgcccg ctccgccatg aggctgtccg cgagcggtcc ggtgacccgg tcgcagcggg
     1441 tgtcgacgcc cgactggtgg atcaggtggg ccaccaccgc gcccgcctgg tcgcggatga
     1501 aaacgagtgt cgtcgaattg tcgttctcga agaagaattt gtcgggggcc tcggggaaga
     1561 cctggaagac cgggccgccg gtccgctgcg agtagaggtt gtcgccgtcg cggcgaatca
     1621 cccggtacga gttgtcgccg acccggtaga cgccgacgag ggcatcgaac gaggcggggt
     1681 cggccgccgc cggtgttttc cggaccggcc ggtcataggg ctggtcgaac atgatcgcgg
     1741 cgagcgtgga gccgaggccg gcggcattgg ccgacatgtt gttgcacagg acgatgatgg
     1801 tcagccggtc gtcggggtag cgggcgatga cgctggtgaa accatcgatg ccgccgccgt
     1861 gggaaatctc gcgccggccg aaccgttccg ccaccatcca gccgtacccg tagttctcca
     1921 gggcgggggt gtacatctgc ttccgggagt cttccgagag aacccggtcg ccgtagagcg
     1981 cctggtccca gatgagcagg tcgccgaccg tggaggcgag ggcgcccgcg gcatacggga
     2041 gggtcatcga gatcggatcg gccgggacga gtgtatcttc cggagccacg tagccgaccg
     2101 cacggttggg ggtccgctcg ttcggggggt aggtcgtgtg cgtcatgccg agggggcgaa
     2161 agatgtgctc cgccaggtaa tcggcgtagg tctgccccga gacagcttcg ataatcagcc
     2221 cgaggaggaa gtagccggag ttgcagtagc ggtacatcgc ccccggctcg aactgcagcg
     2281 gcagatcttt gaaggaagcg acaatttcgc gggccgtcat gtccaagccg cggcgagcca
     2341 tgaggctgtc gatgtcggtg tagctgacta ggccggaggt gtgggtgaga aggtggtgga
     2401 ccgtgattcg gtcgccggtg gctttcggga agtcggggag gaatttcgag atcgggtcgt
     2461 ggacgctgag tgtcccctgc tcctggagct gcaggatggc ggccgcggtg aactgcttgg
     2521 tgaccgagcc gatgaggaac tcggtctcgg gggtgttggg ctggccgtgg gcgacgtcgg
     2581 ccatgccgta gcctttgacg aaaacgggaa cgccgtcgtc ggccacaagc acggctccct
     2641 ggaaccggag gacatcgcag gcggcgctga gataggcatc gagttcggcc gttttgtcgg
     2701 cggccggcac ggtggccgcc agcaggagcg cagcggcgaa caccagcacg ccggcggttg
     2761 tacggagcag gaacgatttg cgcataccgt acctttcaca gcaggataga gcgggttcga
     2821 tttgtcctat cagacgaatc cacgggcgta atgttgccgt caaatgtact gcgcccgtgg
     2881 ggggcgaagc gagcgcctgc tactccatct tctccacgca gccggcgatc aggtcgcgca
     2941 ggatcggctc ggtcgcactc gcggccccga tcacatcggc cagcgagcag ggatgcatgt
     3001 tgtccggcag acccatgtcg gtgatgatcg agaacgcgag gatcttcgtg cgctggtggt
     3061 gggcggtgag cacctccggc accgtcgaca tgccgaccgc atcggcgccg aacccgcgca
     3121 gcatgcggta ctcagcggcg gtctccaggc acggaccggt gagaccgaca tacacgcctt
     3181 cctggaggcg gatcttcagc tcaagagcaa cctgccgggc gagagcctgg taccgccggt
     3241 ggtatacctc gtacatgtcg gggaaacggt cgccgagggt gtcgtcgttg gggccgatga
     3301 gcgggttgcc ggggaagaag ttgatgtggt ctttgatgag catgatatcc cccggcgcga
     3361 aattcgggtt gagaccgccg gcggcgttgg agacaatgag ggtgtggata ccgagcgctt
     3421 tgagggtgcg cacgggaaaa gcgatctgtg gaaacgagta cccctcgtag tagtggaacc
     3481 gtccctgcat gcagaccacc ggtttgccgc gcaggcggcc gaagaggagg cggccggcgt
     3541 gcgactcgac ggtggagacg gggaaatgcg ggatcttctc gtagctgacc gtgccgacca
     3601 tgtcgatgcc gtcgaccagc ttccccaaac cggtgccgag gatgatgccg atccgggggg
     3661 tgaaggcgat ctgcgaggtg atatagccga ccgcgtcgtt cagcatggcg cggaattcct
     3721 ctattgtctg catgggtacg ctccttcagc tgcggaaacc ttccgtttac tgaggcgaat
     3781 atagcaaacg gggggcggga gtcacgcggg aaattgggtc ggaaccgaag ggcccgggcg
     3841 gcgcggacaa ggcggccaag agggcaaagg aaaggaccgg tcgcgggagg gaaggtgcgg
     3901 ccggtccttt aactgcgcgt ggggtgattt tcgcgtcgtt gcgccgccgc taggagcggg
     3961 cggctttgtc catctcctcc tcgaagcgcg cggcgacttc gtcgagttca cgtgcgatgt
     4021 cctcgggcac ggcggcggcg ctcttgcgcg gggc
//
LOCUS       DAOPOE010000167         3953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_24786_length_3953_cov_4.945100, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000167 DAOPOE010000000
VERSION     DAOPOE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24786_length_3953_cov_4.945100"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PKM94_13715"
     CDS             complement(<1..1468)
                     /locus_tag="PKM94_13715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD67734.1"
                     /translation="MDREAGKEEQTEDTRRDERGGVSPSDYIEPEDALPETTLEAQPE
                     RVREAAARAGWSAFMPVQAKTIPYVLARRDLMVQSRTGSGKTGAFLLPILERLEPSRS
                     TCQALILVPTRELARQVAHEAEMLAGGSGVRTAAVYGGVSYRPQIEALTRGSHIVVGT
                     PGRILDHLLKRTFTLDDLDILIFDEADRMLSMGFYPDMIEVQKYLPRRHINSYMFSAT
                     YPMHVINLARQFLRDPGFLSLSKDRVHVAETEHVYYVVPHMEKDRYLVRIIELLNPAA
                     AIIFCNTKNMVHYVTVVLQRFGYDADALSADLSQSQREQVLQRVRDGQLKFLVATDLA
                     ARGIDIPELTHIFQYEPPEDPELYVHRAGRTGRAGATGEAVALVAGMELTELRRIATR
                     FGIDMIERPAPSEDDVVMAVSERMTALLEARLRRRDKLQVERMARFFPLVKLLSENEA
                     GQEVLAMLVDDYYQESLHNPPPLPQEQQQWLIHSSTQHP"
     gene            1716..2231
                     /locus_tag="PKM94_13720"
     CDS             1716..2231
                     /locus_tag="PKM94_13720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD67735.1"
                     /translation="MSTNSAPPRPGVQLVPAPAQDRRVLRRLMSLYLHDLSEYTDFLE
                     PGPDGSFTYEGLDLYWRSPSLIPLFIRSGDRLAGFLLLNKPPYTPHDIDCVVHEFFIL
                     RADRGRGLGRDAAAACFAQYRGRYRLVQLIRNVPAIGFWHSVYAGLNISYEESRQTLA
                     GESFLVQTFTV"
     gene            complement(2299..3102)
                     /locus_tag="PKM94_13725"
     CDS             complement(2299..3102)
                     /locus_tag="PKM94_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HOD67736.1"
                     /translation="MASDSRIEWTESTWNPVTGCARVSPGCDHCYAERMARRLQGMGV
                     EKYKNGFSVTLHPDVLAQPLEWKRPRMVFVNSMSDLFHEQVPLEYVQRIFEVIRRCPQ
                     HVFQALTKRSKRLVRAADKLAWPPNLWMGVTVESATYRFRIDDLRKTPAALKWISFEP
                     LLGSIGDVDLSGIAWAVVGGESGPGARVMREEWALELLRQCRAEGTAFFFKQWGGMYK
                     NRAGRLLRGELYEEMPALPGESGELALAFVAGQQATSTRAGVADTEAGE"
BASE COUNT          661 a         1481 c         1080 g          731 t
ORIGIN      
        1 cggggtgctg ggtgctggaa tgaataagcc actgctgctg ctcctgcggc agcggcggcg
       61 gattgtgcag gctctcctgg tagtaatcat ccacgagcat cgccagcact tcctggcccg
      121 cctcgttctc ggaaagcagc ttgaccagcg ggaagaagcg cgccatccgc tcgacctgaa
      181 gcttgtcgcg gcggcgcagg cgcgcctcga gcagcgccgt catccgctcc gacaccgcca
      241 tcaccacgtc atcttcgctc ggcgccggcc gctcgatcat gtcgatcccg aaccgggttg
      301 cgatccgccg aagttcggtg agctccattc ccgccaccag ggccactgcc tccccggtcg
      361 cccccgcccg ccccgtccgc cccgcccggt ggacatacaa ctccggatcc tccggcggtt
      421 cgtactggaa aatgtgggtc agttccggga tgtcgatccc ccgggccgcg agatcggtcg
      481 ccaccaggaa tttcaactgc ccatcgcgca cccgctggag cacctgctcc cgctggctct
      541 gcgacaggtc ggccgacaag gcgtcggcgt cgtacccgaa ccgctgcagc accaccgtca
      601 catagtgcac catgttcttg gtgttgcaga aaatgatcgc cgccgccgga ttcaaaagct
      661 cgataatccg caccagatag cggtctttct ccatgtgcgg caccacgtag tacacgtgct
      721 cggtctcggc cacgtgcacc cggtccttgc tcagactcag gaaccccgga tcgcgaagga
      781 actgccgcgc gagattgatc acgtgcatcg gataggtcgc cgaaaacatg tacgaattga
      841 tatggcggcg cggaaggtac ttctgcacct caatcatgtc cgggtagaac cccatcgaga
      901 gcatccggtc cgcctcgtcg aaaatgagga tgtcgaggtc gtccagcgtg aacgtccgct
      961 tgagcaggtg gtcgagaatg cgcccgggcg tcccgaccac gatgtgggaa ccgcgggtga
     1021 gcgcctcgat ctggggccgg tagctcacgc cgccgtacac cgccgccgtc cgcaccccgc
     1081 tgccgcccgc gagcatctcc gcttcgtgcg ccacctgccg cgcaagctcc cgcgtcggca
     1141 ccaggatgag cgcctggcag gtcgaccggg acggttccag ccgctccaga atcggaagca
     1201 gaaacgcccc cgtcttcccc gaccccgtgc gcgactgcac catcaggtcc cgccgcgcca
     1261 ggacatacgg aatggtcttg gcctgcaccg gcatgaatgc gctccatccc gctcgcgccg
     1321 ccgcctcccg aacccgctcc ggctgcgcct ccagcgtcgt ctcgggaagc gcgtcctccg
     1381 gctcgatgta atccgatggg cttaccccgc cccgctcgtc ccttcgcgta tcttccgtct
     1441 gctcttcctt ccccgcttca cgatccatca cgcgcacctg actcctttgc tgagggtgtc
     1501 aataaccgtg tcgtttcctt gccgaccggc ttatggtacg tctttttccc gccaaaagac
     1561 agcccttatt tcgccgtccc cggccccccc cggcccagga ccggcgcatt tctcgcgact
     1621 ggcccgaatc tgagaacgaa tgccgacgtg gagcgtacca cggggaaacg gccaaccgcg
     1681 cggcgcccga ccgcccgact gaggatgtcc cgctcatgtc cacgaactcg gcaccgcccc
     1741 gccccggggt tcagctcgtc cctgcgccgg cacaggaccg ccgcgtcctg cgccggctca
     1801 tgtccctcta cctccacgac ctgtccgagt acaccgattt cctcgaaccg ggccccgatg
     1861 gttcgttcac gtacgagggc ctcgacctgt actggcgcag cccctcgctc attcccctgt
     1921 tcatccggag cggtgaccgc ctcgccggtt ttcttttgct caacaagccc ccctacaccc
     1981 ctcacgatat cgactgcgtc gtccacgagt tcttcattct ccgcgccgat cgcgggcggg
     2041 gtctcggtcg cgacgccgcc gctgcctgtt tcgcgcagta ccgcggccgg taccgcctcg
     2101 tgcagttgat ccgcaatgtc cccgccatcg gattctggca cagcgtctac gccggcctca
     2161 acatctccta cgaggaaagc cggcagacgc ttgccggcga gtcgtttctg gtgcagacat
     2221 tcaccgttta gccgcagccc gtgccctatc cgaggcggcg cgtctgccct tggcgacaaa
     2281 ggcgccgccc gcccccggtc actctcccgc ttcggtgtcg gcgaccccgg cgcgcgtcga
     2341 tgtcgcctgc tgccccgcga cgaaggcaag cgccaattcc cccgattccc ccggcagcgc
     2401 cggcatctcc tcgtagagtt ctccccgcag aagtcgcccc gcccggttct tgtacatccc
     2461 tccccactgt ttgaagaaga acgccgtccc ctccgcccgg cattggcgca gcaattccag
     2521 cgcccactcc tcccgcatca cccgcgcccc cggccccgac tccccgccga cgaccgccca
     2581 ggcgatcccg ctcaaatcca catccccgat cgaccccaac agcggctcga acgatatcca
     2641 cttgagcgcc gccggtgtct tgcgaaggtc atctatccga aagcgatagg tcgcactctc
     2701 gaccgtgacc cccatccaga ggttcggtgg ccacgcgagc ttgtcagccg cacgcacaag
     2761 ccgttttgac cgcttggtga gggcttgaaa cacgtgctgc gggcaccgtc ggatcacctc
     2821 gaatatccgt tggacatatt cgagcggcac ctgctcatga aacaggtccg acatcgagtt
     2881 gacaaatacc atacgcggcc gcttccattc aagcggctgg gcgagcacat cggggtggag
     2941 cgtgaccgaa aatccgttct tgtacttctc gacgcccatc ccctgcagcc gccgggccat
     3001 tcgctcggcg tagcagtggt cgcacccggg gctgacgcgc gcgcacccgg tgacggggtt
     3061 ccaggtggac tccgtccact ctatgcggga atcactggcc atggcacgtc aagaaacgaa
     3121 cagttcgctt atcacccaaa gtgaccttgc cctttctttc tctcctctcc cgcggtgcat
     3181 cgaccgacac cagccccctg gactgcaaat tcagccgagc tgtcctcaga gcgttctccg
     3241 gctctttata cgcaagccgt tgacattcgg tgagcagctt acgcactgag ccattgtaat
     3301 gcgtaaagtg cttgagcagg cgctcttcaa gggcgcccat gcgctcactc atctcaaggt
     3361 cggcttgatc agccgacccc attcggatcc cccttagctg gagcacgcgg ggtgccacac
     3421 gctcctcttc ccgtagagcg cgggtgtcat cccatggaag aagaccctca gccaagcttg
     3481 ttctccctac aaggtttcca gatgcggctc ctcggtcggg ttctgcacat tcggctcaat
     3541 tccggtgatc gactgaaaga gcagtcgatc cgcggtgagt ggtcggcggt cagggtcgcc
     3601 acacctccgc tcgcactccg ccggcgcgcg gcccacggga gacctctgga gagctgtcgc
     3661 gcctcaggtt ggcacacccc gccggcgcgc ggctcattcg acacgagcgt cgcattcacc
     3721 gctccgtccc cgcagcatcc cgccggcgcg gcccactccc caccatccgc aaggtcgatc
     3781 tcgaaggcgt gtcgtcaaat cacatattct ctgccaacat aaaaaaacag gccccgtctc
     3841 cgaggcctgc ctccattatc caaactctcc ccgccctcac cgcgtctgaa gcagctcctc
     3901 ctgcggaatg ctgtcggcct ggatcccctt catcgccggc cccagccccc gcg
//
LOCUS       DAOPOE010000168         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_25131_length_3913_cov_4.998445, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000168 DAOPOE010000000
VERSION     DAOPOE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25131_length_3913_cov_4.998445"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKM94_13730"
     CDS             complement(<1..406)
                     /locus_tag="PKM94_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67737.1"
                     /translation="MHYAIKCCAIAVLAAAGCDNPQTPTDNDEEDHREGSLSVVIETT
                     EETFQGSFEDISVSFSGEIDTSFQLGGFDFLLQYGSSALTLQQVSPGSFLQTCGWEYF
                     TYRYGANAICGGAPCPSGVVRVVAIAEMNNGAN"
     gene            complement(610..2919)
                     /locus_tag="PKM94_13735"
     CDS             complement(610..2919)
                     /locus_tag="PKM94_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOD67738.1"
                     /translation="MFFRLVVLLPLLSAAGLLAASSLAVPPDELEPPVFVSPEPRRLL
                     RVSRCDDSAVFIFKAIIPNQPQAQGRVRYRLIDGCGRINEKTGRWVWYPAPEDTARIR
                     RVVVGACINNQDIETLPENRAHLSVLYRDEHARLIWMLGGDTMHAQTPVIAADAPGTT
                     AVTYRISEPDWCDEPAVALRMIDPAPAGKIFFTDSTLTAELAATDAGQRFVVTYGLTS
                     GQFSHEGQVIIDTRRHVVPVFVECPSETLSVAVCATLSCRLRAIDPDFPDDPTGIFYR
                     IAPMPYYPGSVQGSTGEYRFRGRPEDAGQVFEIGVAAHYGDTMTTGDQICRFWVRVDE
                     NQPPLFTGSLCHTLVNAPPGSPPFFEMQVEAVDPEECGPVALFVAGVSPLPVGGFTFD
                     PEDGSFTMPLAPAELEETFTIRIGAADAVDTAYCEFDYRHLNRDPVAVMIERIGGDTP
                     GGRHALDLTVTQNNLELQGFDLTIGFEAAALNLQGVSPGSVYDSCGWEYFTYRYGAGG
                     ACPECPSGILRLVGLAETNNGEAHPSCFRPAAPFSLATLEFLESAEPAWAGVFVPVRF
                     LWLDCTANALAARLIAEIPEYTDLLISREVYDLDSVLAEDGEVYSFLAITDPLGTFPA
                     TGGAVGDCDAAAMPHPAPIRRVDFFNGGVKIAGPPATPVGEHNPDGTAGGPPLPSTFA
                     VHQNCPNPFNLETVIPFDLPRQGRVTLEIYNTLGRRVYRQVGDYPAGYHRLVWDGRTD
                     GGRVAASGVYVYRLATGETAAARKMMLLK"
     gene            3140..>3913
                     /locus_tag="PKM94_13740"
     CDS             3140..>3913
                     /locus_tag="PKM94_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOD67739.1"
                     /translation="MAKDDRKKPEKVKPQVLKGFRDYPPDEQIAREKMLARCREAVEL
                     MGFLPLQTASLEFADTLLGPHYSAENLAELFGFRGPDDVDMALRYEFTVSLARYVAGN
                     PALPLPFRRYQYGNVWRVDKPGPGRFREFMQFDIDIVGTRNLLADAEIIAAMVKTLDH
                     LGIGKFKVRYSTRRLLNGLIEFAGIPAAVGPDVMRVIDKLEKQGRAAVVLELGPGRTD
                     ASGDRIPGLGLADGQIAQIEKFLEIAGQHDRDHLPEAERL"
BASE COUNT          744 a         1265 c         1244 g          660 t
ORIGIN      
        1 agttggcgcc gttgttcatc tcggcgatcg ccaccacccg caccacgccg ctcggacagg
       61 gcgcaccgcc gcatatggcg ttggccccgt agcggtaggt aaagtactcc cagccgcagg
      121 tctgcaggaa gctccccggc gagacctgct gcagcgtcag cgcgctggaa ccgtactgaa
      181 gcaggaaatc aaatccaccc aactggaacg aggtgtcaat ttcgccactg aaggaaacgg
      241 agatatcttc gaaactgccc tgaaatgttt cctcggtcgt ctcaatgacc acgctgagcg
      301 aaccttcccg gtggtcttcc tcgtcgttgt cggttggagt ttgcggatta tcgcagcccg
      361 ccgccgccag aacggcgatt gcacagcact tgatggcata atgcatagcc ggtgactcct
      421 tgcgccacga tgttgtttgc agagaagcct gcttcccgct ggtgcgggta gacgataatc
      481 tagggcggcg agatagttcc gtcaaggcgg aatccgtgcc tccgcgctga caggtcaggt
      541 acctgggtgc cggttcgccg agcaggccgg ggaagcccgc tccgaagcgg ggggcagccc
      601 ccccgccttc tacttcagga gcatcatctt gcgggcagcc gcggtttccc ccgtcgcgag
      661 gcggtacacg tacactccgc tggcggcgac ccggccgccg tcggtcctcc catcccatac
      721 caaccggtgg tatccggccg gatagtcccc aacctggcgg tacacccgcc gcccaagcgt
      781 gttgtagatc tcgagcgtga cccgaccctg cctcggcaga tcgaacggaa tcacggtttc
      841 aagattgaac ggattggggc agttctggtg gacggcaaac gtcgagggca gcggcggtcc
      901 cccggctgtc ccgtccggat tatgctcccc gaccggtgtg gctggcgggc cggctatctt
      961 cacaccgccg ttgaagaagt ccacccgtcg aatcggggcc gggtgcggca tcgcagccgc
     1021 atcgcagtca ccgaccgccc cgccggtcgc ggggaacgta ccgagcgggt cggtgatcgc
     1081 caggaacgaa tagacttcgc cgtcttccgc cagaacgctg tcaaggtcat acacctcccg
     1141 tgatatcagg aggtcggtgt actctgggat ctcggcgatg aggcgcgcgg cgagggcgtt
     1201 ggcggtgcaa tccagccaga ggaagcggac cggcacgaac acgcccgccc aggcgggttc
     1261 ggcgctttcc agaaactcga gtgtcgccag cgaaaacggc gcggccgggc ggaaacacga
     1321 agggtgcgcc tccccgttgt tggtttcggc caggccaacc agtctgagga tgcccgaggg
     1381 acactcgggg caggcccctc cggcgccgta gcggtaagta aaatactccc agccgcacga
     1441 atcataaacg gagccgggcg acaccccctg caggttaagg gccgccgcct cgaaaccgat
     1501 ggtgaggtca aaaccctgga gctccaggtt gttttgggtg accgtgaggt ccaaagcatg
     1561 ccggccgccg ggtgtatcgc cgccgatccg ctcgatcatc acggccacag ggtcccggtt
     1621 caggtggcgg tagtcgaatt cgcaatacgc cgtatcaacc gcatcggcgg cgccgatccg
     1681 gatggtgaaa gtctcttcaa gctccgccgg ggccagcggc atcgtgaaag atccgtcctc
     1741 agggtcaaac gtgaaaccgc cgacgggcag aggggagacg ccggcgacaa acagcgcgac
     1801 cggaccgcat tcctccggat cgacggcctc cacctgcatc tcgaagaagg gcgggctgcc
     1861 ggggggagcg ttcaccagcg tgtggcacag cgagccggta aacaggggcg gctggttctc
     1921 gtccacccgg acccagaacc ggcagatctg gtccccggtc gtcatggtgt cgccgtagtg
     1981 ggccgccacg ccgatttcga acacctgccc ggcatcctcg ggccgccccc ggaagcgata
     2041 ctcgcccgta gagccttgca cgctgccggg gtagtaaggc atgggcgcga tgcggtagaa
     2101 gattcctgtc ggatcatccg gaaagtcggg atcgatcgcc cgcagccggc aggagagggt
     2161 ggcgcagacg gccaccgaca gagtctcgga ggggcattcg acaaacaccg gcacaacatg
     2221 gcgccgggta tcgataataa cctgaccttc atgggagaac tgcccggacg tcagcccgta
     2281 cgtgacgacg aacctctggc ccgcgtcggt cgccgccagc tcggccgtca gggtcgagtc
     2341 ggtgaagaat atcttccccg ccggcgccgg atcgatcatt cgaagcgcca ccgccggctc
     2401 gtcgcaccag tccggttcag agatgcggta ggtcactgcc gtagtccccg gggcgtcagc
     2461 cgcaatcacc ggcgtctgcg cgtgcatggt gtcgccgccg agcatccaga tcagccgggc
     2521 gtgctcgtcg cggtatagca cgctcaggtg ggcccggttt tccgggagcg tctcaatgtc
     2581 ctggttgttg atacaggcgc cgacgaccac cctgcggatc cgcgccgtgt cttcgggcgc
     2641 cggataccac acccatcggc cggtcttctc gttgatccgg ccgcacccgt cgatcagccg
     2701 gtagcgcacc cgtccctggg cctggggctg gttgggaatg attgccttga aaatgaaaac
     2761 ggcggagtcg tcgcagcggg atacccgcag cagccgccgg ggctccggtg aaacgaacac
     2821 cggcggctcg agttcatcgg gcggaacagc caggctcgac gcagccagga gaccggccgc
     2881 cgacagcagt ggtagcaaga caactaatcg aaagaacatg cgcccttccc ctcgttcata
     2941 gttccgtgat gttcgtgcta tcatcgacaa acatacctaa tttccgagaa atgtcaatgc
     3001 aaatacaccg atactactgc cgccggatgg tcaaccggag accgataggg aaggaaaaaa
     3061 tgcttttctt tgggtcgcga ttgcctacat ttacccgcga gagtcggtga ccggaagccc
     3121 agcaacaagt taggagacca tggccaaaga cgaccgcaag aagccggaaa aggtaaagcc
     3181 gcaggtgctc aagggatttc gcgactaccc gcccgatgag cagattgccc gcgagaagat
     3241 gctcgccagg tgccgcgagg cggttgagct gatggggttt ttgcctctcc agaccgcgtc
     3301 gctggagttc gccgacacgc tgctcgggcc ccactacagc gcggagaacc tcgccgagct
     3361 gttcggtttt cgcggtcccg atgacgtcga catggctctt cgctacgagt tcacggtctc
     3421 gctggcccgc tatgtggccg gcaacccggc gctgcccctg ccgttccgcc gctaccagta
     3481 cggcaacgtg tggcgggtgg acaagccggg cccggggcgg tttcgcgagt tcatgcagtt
     3541 cgacatcgac atcgtcggca cgcgaaacct cctggccgac gcggagatca tcgccgctat
     3601 ggtgaagacc ctcgaccatc tgggcatcgg caagttcaag gtccgctact cgacccgccg
     3661 gcttctcaac ggcctgattg aattcgccgg catccccgcc gccgtcggcc ccgacgtcat
     3721 gcgcgtgatc gataagctcg aaaaacaggg gagagcggcg gtggtgctcg agctcggtcc
     3781 cggccggacc gatgcctccg gcgaccgaat ccccggtctc ggcctcgccg acggccagat
     3841 cgcccagatt gagaagttcc tcgagatcgc cgggcagcac gaccgcgacc atcttcccga
     3901 ggccgaacgc ctg
//
LOCUS       DAOPOE010000169         3912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_25139_length_3912_cov_5.353643, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000169 DAOPOE010000000
VERSION     DAOPOE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25139_length_3912_cov_5.353643"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKM94_13745"
     CDS             <1..232
                     /locus_tag="PKM94_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67740.1"
                     /translation="GESMRKLFVTALLVFAVAMVFGFASGQVAAKGGGGGGTSCYYYC
                     TCEGNPMKCCLFNGVYFCFPTEEIQCTQIYEC"
     gene            337..906
                     /locus_tag="PKM94_13750"
     CDS             337..906
                     /locus_tag="PKM94_13750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD67741.1"
                     /translation="MPASPLRLLRLPVAAGLIGLASLFGIYIGTATRSVVEKPAPGDL
                     LPKLSLAVGEAFPEIPLVDSAGRGLSSGQAIGSDGAVIVFAYLDCPPCEQMIRRIQDL
                     IDRKILADTQVLLIGPTTAAAHLPAFHRRYGWTFPAYADTGAVAMQRYGVEGFPLLAV
                     VGPDRLIRHIRTDARMPVDPAQVKAWLSD"
     gene            1034..2401
                     /gene="glmM"
                     /locus_tag="PKM94_13755"
     CDS             1034..2401
                     /gene="glmM"
                     /locus_tag="PKM94_13755"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD67742.1"
                     /translation="MTEQTLITSTSGIRGIVGSGLNPVLAASYGAAFGTFLTRGKVVI
                     GRDSRPSGELLMRAVVAGLASVGIDVIEIGVVPTPTVEIAVKESKAAGGICITASHNP
                     SPWNALKFFNRTGEFITPAQFRELDELFRSGRFGYRPYDRLGSIDRQEGWIDWHIQKT
                     LAVPFVNRAAVRRRKFRVVVDAINGAGSTALPALLRKLGAGVVEVNCKGDGDFVHEPE
                     PIPKNLRQLSLMVRKHKADIGMACDPDADRLALVDERGRPIGEELTLALSVQQVLTRK
                     KGPVVINLSTSKVTERVARDRGAAVYYAKVGEANVVELMRAKRALVGGEGNGGVILPS
                     FHAGRDALMAAALVLSRLAEANVEFSSLADSLPTYYTRKDKGTVPADFSARLARFERE
                     APGLLGEFAIDRQDGLRVDFSGGWVQIRTSNTEPIFRLIVETESKDRTEHLADRVMGY
                     FREPR"
     gene            2408..>3912
                     /gene="glmS"
                     /locus_tag="PKM94_13760"
     CDS             2408..>3912
                     /gene="glmS"
                     /locus_tag="PKM94_13760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOD67743.1"
                     /translation="MCGIVGYVGPKQAKPILLQGLKRLEYRGYDSSGIALLTERGLMV
                     AKSAGKIANLEKMLSENEFDSSLCTHGIAHTRWATHGEPNQLNAHPHTDGRGEIALVH
                     NGIIENYRALKEFLQRQGFELKTETDTEVLVQLIRYYYRDDLTEAVCEALAQVEGTYG
                     IAVISARHPNTIVAARQGSPLVLGIGEKEKFVASDVAAMLDHTNKIIYLDEGEIATIT
                     DCSYEITTIQRDKITPRIEEVSWTLDQIEKEGYDHFMLKEIYEQPVTLRNAMRGRINL
                     DEGIPRLGGLNLQYEELRRIKRLIFTACGTSWHAALVGEYLIEELAGIPVEVEYASEF
                     RYRSPIFPEDTILFGISQSGETADTLAAMREAKKRGVTVLGIVNVVGSTIARETDGGV
                     YIHAGPEIGVASTKAFTSQVMAISLIGTLLARMRHLSVPQGQEMLGHLDRIPEQVDRI
                     LKNDQAIREIAERYHRADNFLYLGRGINFPVALEGALKLKEISYIHAEGYPA"
BASE COUNT          706 a         1245 c         1272 g          689 t
ORIGIN      
        1 aggtgaatca atgcgcaagc tgttcgttac ggccctgctg gtgttcgcgg tggcgatggt
       61 gttcggtttc gcgagcggcc aggttgcggc gaagggcggc ggcggtggcg gcacgagctg
      121 ctactactac tgcacctgtg aaggcaatcc gatgaagtgc tgcctgttca acggcgtgta
      181 cttctgcttc ccgacggaag agattcagtg tacgcagatc tatgagtgct aggcgtctgt
      241 tcgccttcgc actcggaatc tagcggttgt gaaacgccgc cggcgggcga ccgccggcgg
      301 cgcgctcagg aacgatcccg aaagcgctcg cccaccatgc ccgcctcccc gctccgactg
      361 cttcgcctgc cggtcgcggc cggcctcatc ggcctcgcct cgctcttcgg catctatatc
      421 ggcacggcga cccgcagcgt ggtcgaaaag cccgctccgg gcgacctgct gcccaagctc
      481 agcctcgcgg tcggggaggc cttccccgag atcccgctgg tcgacagcgc cggccggggg
      541 ctgtcgagcg gacaggcgat tggttccgac ggcgcggtga tcgtcttcgc ctacctcgac
      601 tgcccgccct gcgagcaaat gatcaggagg attcaggatc tcattgaccg gaagatcctg
      661 gccgacacgc aggtgctgct catcggcccc accaccgctg cggctcacct gccggctttt
      721 caccggcgct acggctggac gttcccggcc tacgccgata ccggggctgt ggcgatgcag
      781 cggtacggcg tcgaggggtt cccgctgctg gcggtggtcg ggcccgaccg gttaatccgg
      841 catattcgaa ccgatgcccg gatgccggtg gatcccgcac aggtgaaggc ttggctgagc
      901 gactgatgcg gacgtccgtg accacccgcc gaggaacatc cggccgcccg gttctgttct
      961 tgtgttgccc gtggttgcgc tgtagattgg cctctcacag gaacgtgcgc gacagcaaat
     1021 ttcggcagga gtcatgacgg agcaaactct catcacctcc acctccggga tccgcggtat
     1081 tgtcggttcc ggactcaatc cggtgctcgc ggcgtcgtac ggcgcggctt tcggcacctt
     1141 tctcacgcgc ggcaaggtgg taatcggccg cgatagccgg ccctcgggcg aattgctcat
     1201 gcgcgccgtg gtcgccggcc tggcctcggt cgggatcgac gtcatcgaga tcggggtggt
     1261 cccgacgccg accgtggaga tcgcggtcaa ggagtcgaaa gcggccggcg gcatctgcat
     1321 caccgcctcc cacaacccgt cgccgtggaa cgcgctgaag tttttcaacc ggaccggcga
     1381 gttcatcacc ccggcccagt tccgggaact cgacgagctt ttccggtcgg ggcgtttcgg
     1441 ctaccggccg tatgaccgcc tggggtcaat cgaccggcag gagggttgga tcgactggca
     1501 catccagaag actctggcgg taccattcgt gaatcgggcg gcggttcggc gccggaagtt
     1561 ccgggtggtg gtggacgcta ttaatggtgc aggctcgacg gcactgcccg ccctgctgcg
     1621 aaagctgggg gccggggtgg tcgaggttaa ctgcaagggg gacggcgact ttgtccatga
     1681 accggagccg attcccaaga atttgcggca gctcagcctt atggtccgta agcataaggc
     1741 ggacatagga atggcttgcg accccgatgc tgaccggctg gcgctggtcg atgagcgggg
     1801 acgcccgatc ggcgaggaac tgactctggc tctttcggtt cagcaggtcc tgacccggaa
     1861 aaagggcccg gtcgtcatca atttgtcaac ctcgaaagta accgaacggg tcgcccgcga
     1921 ccggggcgca gcggtgtact atgcgaaagt gggcgaagcg aatgtcgtcg agctcatgcg
     1981 ggcgaaacgg gcgctggtcg gaggcgaggg gaacggggga gtgattttgc cgtcgttcca
     2041 tgccggccgg gacgccctca tggcggctgc cctggtgctg tcccgtctcg ccgaggcaaa
     2101 cgtggagttt tcaagtcttg ccgattccct tccgacctat tatactagaa aggacaaggg
     2161 gaccgtaccc gccgacttct ctgcgcggct cgcccggttt gagcgggagg cgccgggact
     2221 gctcggggaa tttgccatcg atcgacagga cggattgcga gtggatttct cgggcggatg
     2281 ggtccagatc cgcacctcga atactgagcc gattttccgc ctgattgtag aaacggaaag
     2341 caaggacagg actgaacacc tggcagaccg ggtgatggga tattttcgcg agccgaggta
     2401 agagcctatg tgcggtatcg tgggttacgt ggggccgaag caggccaagc cgattctcct
     2461 gcaggggttg aagcgcctgg agtatcgggg atacgattcg tcggggatcg ccctgttgac
     2521 cgagcgcggc ctgatggtgg cgaagtcggc ggggaagatc gccaatttgg agaagatgct
     2581 cagcgagaat gagttcgaca gcagcctctg cacgcacggc atcgcccaca cccgctgggc
     2641 cacccacggg gaacccaacc agctcaacgc ccacccccac accgacggcc ggggcgaaat
     2701 cgcccttgtc cacaacggga tcatcgaaaa ctaccgcgcc ctcaaagaat tcctccagcg
     2761 ccagggattc gaactcaaga ctgagaccga caccgaggtc ctcgtccagc tgatccgcta
     2821 ctactaccgc gacgatctca ccgaggcggt gtgcgaggcg ctcgcccagg tcgaagggac
     2881 ctacgggatc gccgtcatct ccgcccgcca ccccaacacc attgtcgccg cgcgccaggg
     2941 ctcccccctc gtgctcggca tcggcgagaa ggagaagttc gttgcctccg atgtcgccgc
     3001 catgctcgac cacaccaaca agatcatcta cctcgacgag ggggagatcg cgacgatcac
     3061 tgactgctcg tacgagatca cgacgatcca gcgcgacaag atcactccgc ggatcgagga
     3121 agtgtcgtgg acgctcgacc agatcgagaa agagggctac gaccacttca tgctcaagga
     3181 gatctacgag cagccggtca ctctccgcaa cgccatgcgc gggcgcatca atctcgacga
     3241 gggcattccc cggctgggcg ggctgaacct ccagtacgag gaactgcgcc ggatcaagcg
     3301 gctcatcttc accgcctgcg gtacctcctg gcacgcggcc ctggtcggcg agtacctcat
     3361 cgaggaactg gccggcatcc cggtggaggt cgagtacgcg agcgagttcc gctaccgctc
     3421 gccgattttc ccggaggaca cgatcctgtt cggcatttcg cagtcggggg agacggccga
     3481 cacgctcgcg gcgatgcgcg aggcgaagaa gcgcggcgtc accgtcctcg gcatcgtcaa
     3541 cgtcgtcggc tcgaccatcg cgcgcgagac cgacggcggg gtgtacattc acgccgggcc
     3601 ggagatcggg gtcgcctcga ccaaggcgtt cacctcgcag gtgatggcga tctcgctcat
     3661 cggcacgctt ctggcccgga tgcgccacct ctcggtgccg cagggacagg aaatgctcgg
     3721 ccacctggac cgcattcccg agcaggtcga ccggatcctc aagaacgacc aggccatcag
     3781 ggaaatcgcc gagcgctacc accgcgccga caacttcctc tacctcggcc gcgggatcaa
     3841 cttcccggtc gcgctcgagg gggcgctcaa gctcaaggaa atctcctaca tccacgccga
     3901 gggctacccg gc
//
LOCUS       DAOPOE010000170         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_26689_length_3743_cov_4.396963, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000170 DAOPOE010000000
VERSION     DAOPOE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26689_length_3743_cov_4.396963"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKM94_13765"
     CDS             complement(<1..452)
                     /locus_tag="PKM94_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD67744.1"
                     /translation="MSAEPGGTPYIIAGWEITSQCNLRCPHCYTAARTRPHDEMGTEV
                     CKGVIEAMAEIGVRMIGWTGGEPLLREDLEELTAYAGERGINCNITTNGVLLDRRRAE
                     GLVAAGLRAFQISLDGSTPERNTRMRGTDEAQFEKIVAGIRVCKELGAR"
     gene            complement(449..1786)
                     /locus_tag="PKM94_13770"
     CDS             complement(449..1786)
                     /locus_tag="PKM94_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD67745.1"
                     /translation="MKPSPYNHFIPARDGIILAYNSYSGALAEIEPEHYGRVQELLAD
                     PGLSASAQDAEFVACLQAGGFLIPDGIDQFGALRSAAKQVRLGGVVLTLTIAPTLACN
                     FACDYCFESRSAVTMSPEVQEALLRFAERQLVRSEMLRVCWFGGEPTLCLPLVERLQR
                     QLLELAAKRRANVIPGSIISNGYLLDGAAAQRLKELGIERAQVTIDGPEGVHDARRKL
                     RSGKGTFQRIIANLRETAGILDINVRINMDKENIDSACEVMALLDREGILPRVKVQFA
                     QVRSTGAACSDTRDRCYTDEEFARTQVELYRQLLRRGIRQVEYPQALGGAGYCSALSE
                     THYVVSPQGYLFRCWEELSTDPARSIGSLLQTAPEDYQAANAKSYRDWDPYALGECRA
                     CAVLPLCAGGCPLRAKEKRGEQRGECVTWKYNLPGMVAVSYEAQVQAPTGADQ"
     gene            2577..3722
                     /gene="hppD"
                     /locus_tag="PKM94_13775"
     CDS             2577..3722
                     /gene="hppD"
                     /locus_tag="PKM94_13775"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOD67746.1"
                     /translation="MSSPLGIRGYDYVEFYVGSARMTAFWFARALGLQLTGYAGPETG
                     VRDRISFYLTRHRLKFVVTSPLQPDTWDIWGWITKHGDGVKRWAVEVDSVEDAFAYAA
                     KRGAVMVKRPERISNRHGYIDEAAIRLYDDTELVYVNRDHYRHIFRPGFCEPLFSFDA
                     GRDDTGLLAVDHIVGNVRENEMNLWAEYFNNTMDFETFVDFGPGDISTRYSSLLSKVV
                     RSKDSKIRNPINEPYEGLKKSQIEEFIEQYRGSGIQHVALRTGDIVATVSALRRNGVE
                     FLAVPDAYYDRLRGRSEVMSRLREDLALLKQHGILCDIESDGYLLQLFTKPIGDRPTF
                     FFEVIQRVGNSEGFGKGNFQALFESIELDQKLRGNLEKDKVETAPIC"
BASE COUNT          618 a         1431 c         1032 g          662 t
ORIGIN      
        1 cgcgctccga gctctttgca gacgcggatc cccgcgacta tcttctcgaa ctgggcctcg
       61 tccgtccccc gcatccgcgt attccgctcg ggcgtggatc cgtcaaggct gatctggaaa
      121 gcgcgcaggc cggccgccac cagtccctcc gcccgccgcc ggtcaagcag caccccgttg
      181 gtcgtgatgt tgcagttgat cccccgctcc ccggcgtagg cggtcagttc ctcgaggtcc
      241 tcccggagga gcggttcccc gcccgtccag ccgatcatgc gcaccccgat ttccgccatg
      301 gcctcgatca cccccttgca gacctccgtc cccatctcgt cgtggggccg cgtccgggcc
      361 gccgtgtagc agtgcgggca gcgcaggttg cactggctgg tgatctccca ccccgcgatg
      421 atgtaggggg tgccgccggg ctcggcgctc attggtccgc ccctgtcggc gcctgcacct
      481 gcgcctcata cgacacggcc accatccccg ggagattata tttccaggtc acgcactcgc
      541 cgcgctgctc cccccgtttc tccttggccc gcagcgggca gcccccggcg cagagcggga
      601 gaacggcgca ggcccggcat tcgcccaggg cgtagggatc ccagtcgcgg tagcttttcg
      661 cgttggccgc ctggtagtcc tccggcgccg tctgaagaag actcccgatc gaccgcgccg
      721 gatcggtcga cagctcctcc cagcaccgga agagataccc ctgcggcgag accacgtagt
      781 gcgtctccga cagcgcgctg cagtaccccg cgcccccgag cgcctgcggg tactccacct
      841 ggcggatgcc ccgccggagc agttgccggt agagctccac ctgcgtccgg gcgaactcct
      901 cgtccgtgta gcaccggtcg cgcgtgtcgc tgcaggccgc cccggtcgac cgcacctgcg
      961 caaactgcac cttcacgcgc ggcaggatcc cctcccggtc caaaagcgcc atcacctcgc
     1021 aggccgaatc gatattttcc ttgtccatgt tgatgcgcac gttgatgtcg agaatccccg
     1081 ccgtctcgcg cagattcgcg ataatccgct gaaacgtccc cttgccgctg cgcagcttcc
     1141 gccgggcatc gtgcaccccc tccggaccgt cgatcgtcac ctgcgcccgt tcaatcccca
     1201 gctctttcag ccgctgcgcc gccgccccgt cgaggaggta gccgttgctg atgatcgacc
     1261 ccgggatcac gttggcccgc cgtttcgccg ccagctccag caactgccgc tgcagccgct
     1321 cgaccagcgg caggcagagc gtcggctccc cgccgaacca gcacacgcgc agcatttccg
     1381 accgcacgag ttgccgctcg gcgaaccgca gcagcgcctc ctgcacctcc gggctcatcg
     1441 tcaccgccga acgcgattca aagcagtagt cgcaggcaaa gttgcacgcc agcgtcggcg
     1501 caatcgtcag cgtcaacacc acccccccga gacgcacctg cttggccgcc gaccgcagcg
     1561 ccccgaactg gtcgatcccg tcgggaatta aaaacccgcc cgcctgcagg caggccacga
     1621 actccgcgtc ctgcgcgctc gcggacaggc ccgggtcggc gagcagctcc tggacccgcc
     1681 catagtgctc cggctcgatc tcggccagcg cccccgaata actgttgtag gccaggatga
     1741 tcccgtcccg ggccgggatg aagtgattgt acggcgacgg cttcacggcg tcctctcaca
     1801 atgctgtcgg gttgccccgc ctgtgctcat gtgacgctgc accggccccg atggccggtg
     1861 cagcttcgac tcaaacggcc gttctcacca cactccgcaa atgatcttca acggaccatt
     1921 caccttgcag atgatgtagt gcggcctctt caccatcaga tcgttgggca gtctgatgag
     1981 cttcatatgc acctccttct tggagttatt gcttccccgc cccggtcggt ctggccggcg
     2041 cgggacctca ggccactgtc agttatcgcg aaccgagcgc cgcttcgcac cgtcggcgcg
     2101 acgccctcta tcatttccgt gccaacgggc tgctccggcg gccgcgtttg ccgtgcgatt
     2161 cgtactcatt aaacttccgt gataatccgg ccggccgatt gagacgccgg ccccgacctg
     2221 tccgccctcc ccgccatccg ggtaccgcct cttcctcctc gcctggcttc ccccgccaga
     2281 cggaatgttt ccccttctgc cccgccccga accagtctgt tgacctttta gaatacactg
     2341 aaaatagtct tgtgtcaagc cgatagtcaa accggccgct ccctgcggcc ggcccccggc
     2401 agcggttttc cgggccggcc gaccacgcgc gccggtgccc gtttggccgg gaacaatccc
     2461 aagcctatcc tgtttttgcc gttagtagcg gcgcccgcgg cccgtgccgc cgcccccggc
     2521 ggccgtcgct gcgttgccga cccgatcccg aaaaaccgat agagaggaga cttgacatga
     2581 gcagcccgct cggtattcgc ggttacgact acgtggaatt ctatgtcggc tccgcccgca
     2641 tgaccgcttt ctggttcgcc cgcgccctcg gcctccagct caccggctat gccggccccg
     2701 aaaccggcgt ccgcgaccgc atctccttct atctcacccg ccaccgcctc aaattcgtcg
     2761 tcacctcccc cctccagccc gacacctggg acatctgggg gtggatcacg aagcatggcg
     2821 acggggtcaa acgctgggcg gtcgaggtcg attccgtgga ggacgccttc gcctacgccg
     2881 ccaaacgggg cgccgtcatg gtcaaacgcc ccgaacgcat ctccaatcgc cacggctaca
     2941 tcgacgaagc cgccatccgc ctctacgacg acaccgaact cgtctacgtc aaccgcgacc
     3001 actaccgcca catcttccgc cccggcttct gcgaacccct cttctccttc gacgccgggc
     3061 gcgacgacac cggcctgctc gccgtcgatc acatcgtcgg caacgtccgc gagaacgaga
     3121 tgaacctctg ggccgagtat ttcaacaaca ccatggattt cgaaaccttc gtcgatttcg
     3181 gccccggcga catcagcacc aggtattcct ccctgctgtc gaaagttgtg cgctcgaaag
     3241 actcaaaaat ccgcaacccc atcaacgaac cgtacgaggg actcaagaag tcccagatcg
     3301 aggagttcat cgagcagtac cgcggctccg gcatccagca cgtcgccctc cgcaccggcg
     3361 acatcgtcgc caccgtgtcc gccctccgtc gcaacggcgt cgagttcctg gctgtccccg
     3421 acgcctacta cgaccggctc cgcggccgct ccgaggtcat gtcccgcctg cgggaggacc
     3481 tcgccctgct caaacagcac ggcatcctct gcgacatcga gagcgacggc tacctgctcc
     3541 agctcttcac caaaccgatc ggcgaccgcc cgaccttctt cttcgaagta atccagcgcg
     3601 tcggcaacag cgaggggttc ggcaagggca acttccaggc gctcttcgaa tccatcgaac
     3661 tcgaccagaa actccggggc aacctcgaaa aggacaaagt ggaaaccgcg ccaatctgct
     3721 gatcggcgcc tcccgctgaa gga
//
LOCUS       DAOPOE010000171         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_27502_length_3657_cov_4.895058, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000171 DAOPOE010000000
VERSION     DAOPOE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27502_length_3657_cov_4.895058"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..2306
                     /gene="fusA"
                     /locus_tag="PKM94_13780"
     CDS             222..2306
                     /gene="fusA"
                     /locus_tag="PKM94_13780"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD67747.1"
                     /translation="MKEYPTDKIRNICLAGQRGCGKTSLADALAYATGVNNRIGRVDD
                     GSSLLDYTDSEISRRTSIQLKLLAMAWKDCKLNLFDAPGHLDFMGEFLACAKVSDVVC
                     FVVNATAGVEVGTQLQWRSLADSPHARFIFVNKMEIENAKWQTTVESIQAAFGNHAVP
                     VTIPIGEADKFRGMIDLLHMKAYEYAPDGTRKEIDIPADLKDRAQADREALVEMAAEA
                     SDELMEKFFDQGTLPEEDVRIGLRQGIARGKLYPILCGAAGKNWGTGVLLDFAVEFLP
                     SPDQMPAPQAQKTGAADLVEVRCDPNGPPALFVFKTITEGHLGELAFFRVYSGAVSSG
                     MDFVNQRTRNSERATQIYTQQGKNRSELSKVVAGDVGALVKLRNTNTGDTLTGGNLSL
                     VIPPVRYPNPVMDVAIRPKSKGDEEKIGTGLQKISQEDPTFRIIHDAALKQMVLVGQG
                     ATQIEIITEKLKKRYGVEVDLVRPRIPYRETIRAKAETQYRHKKQSGGRGQFGDVHLR
                     LEPNERGKGFEFLDEIKGGVIPNKYIPAVEKGVVEAMERGPLTGSQVIDVKVAVFYGS
                     YHEVDSSDMAFKIASSMAFKQGFMQAKPVLLEPIYNVEVLVPEDFTGDVMGDLSARRG
                     KIAGMDPAGTHQRIRAQVPQAEMYQYSVDLRSMTQGQGVYTMEFSHYEEVPHETAQKV
                     IEEAKAAAEEAS"
     gene            2487..3104
                     /gene="lexA"
                     /locus_tag="PKM94_13785"
     CDS             2487..3104
                     /gene="lexA"
                     /locus_tag="PKM94_13785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOD67748.1"
                     /translation="MPRADLTDKQRAVLAYIERQIAERGHSPTIREIGQQFGIASTNG
                     VRTHLTALIRKGYLKKEAQISRGLAPTRTLALRVGRVPLVGTVPAGRPIDAVENVEGE
                     IAVDESFLPKEDSFSLRVAGNSMQGAGILDGDIVLVKKQRVAQKGDIVVAIIGGEATV
                     KRYFPEGNRVRLQPENDDFEPIFVDRKSDEFRIAGKVIGLLRRLK"
     gene            complement(3193..>3657)
                     /locus_tag="PKM94_13790"
     CDS             complement(3193..>3657)
                     /locus_tag="PKM94_13790"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOD67749.1"
                     /translation="FDGNLEMAQAADGKLELASAKVSMTAKAASINTDNDKRDEHLRS
                     ADFFDAATYPELTFVSKKVIPGEGDKFKLIGDLTIRGVTKEVTFDGTLNGVVTDFMGN
                     TRAGFSAAATINRQDFGVSWSKLLDNGGLVAGDNVDIIIEIEAVLPKPESKG"
BASE COUNT          716 a         1069 c         1227 g          645 t
ORIGIN      
        1 gggcttcggc tattaccaca aacccccgcc accgccaccg cgccggcgcg cctgtccgcg
       61 cccttccccg ccggccgatt tcccgggcgg gacggccaaa ggcgttgact tttcagcccc
      121 tgtttggtat gtatggagtt cgcggtttcg ggcggaaagc ccgcaacgga aagaacggac
      181 ggaacataga atttatgata cgatagtttg gagggtccct ggtgaaggaa tatccgaccg
      241 acaaaatccg caacatctgc cttgccgggc agcggggatg cggcaagacc agcctggccg
      301 atgcgctggc gtacgccaca ggcgtaaaca accggatcgg gcgggtcgac gacggttcct
      361 cgctgctgga ttacaccgac agcgaaattt cgcgccggac atcgattcag ctgaagctgc
      421 tggcgatggc gtggaaagac tgcaaactca atctgttcga cgccccgggg cacctggact
      481 tcatgggcga attcctcgcc tgcgccaaag tctccgacgt cgtctgtttc gtcgtgaacg
      541 ccaccgccgg cgtggaagtc gggacacaac tgcagtggcg gtcgctcgcc gattccccgc
      601 atgcccgctt cattttcgtc aacaagatgg agatcgagaa cgccaagtgg cagacgaccg
      661 tggagtcgat ccaggcggcc ttcggcaacc acgccgtgcc ggtgactatc ccgatcgggg
      721 aggcggacaa attccggggc atgatcgacc tgctgcacat gaaggcgtac gagtatgcgc
      781 cggacgggac gcgcaaggag atcgatattc cggctgacct gaaggaccgg gctcaggccg
      841 atcgcgaagc gctcgtcgag atggcggccg aagccagcga cgagctgatg gaaaagtttt
      901 tcgaccaggg gacgctaccc gaagaggatg tccggatagg gctgcggcag gggattgcgc
      961 gcggcaagct ctaccccatt ctctgcggcg cggcgggcaa gaactggggc accggggtgc
     1021 tcctggattt cgcggtcgag ttcctgccgt cgccggacca gatgccggcg ccgcaggccc
     1081 agaagaccgg cgccgccgat ttggtcgagg tgcggtgcga tccgaacggc ccgccggcgc
     1141 tgttcgtctt caagacgatc accgaaggcc acctcgggga actggcgttt ttcagagtgt
     1201 actcgggagc ggtctcctcg ggcatggatt tcgtcaacca gcggacgcgc aactccgagc
     1261 gggccaccca gatatacacg cagcagggga agaaccgatc ggagctgagc aaagttgtgg
     1321 ccggggacgt cggagcgctg gtaaaactgc gcaacaccaa taccggcgat acgcttacag
     1381 gcggcaatct cagcctcgtc attccgccgg tgcgctaccc caacccggtg atggacgtgg
     1441 cgatcaggcc caagtccaaa ggggatgaag agaagatcgg gaccggcctg cagaaaatca
     1501 gccaggagga tccgacgttc aggatcatcc acgacgccgc tctcaagcaa atggttctcg
     1561 tcgggcaggg ggccacccag atcgagatta tcaccgagaa gctgaagaag cggtacgggg
     1621 tggaagtcga cctggtgcgg ccgcgcatcc cctaccggga gacgatccgg gcgaaggcgg
     1681 agacgcagta ccgccacaag aagcagtcgg gcgggcgggg gcagttcggc gacgtacacc
     1741 tgcggctgga gcccaacgag cgcggcaagg ggttcgagtt cctcgacgag atcaagggcg
     1801 gggtgatccc gaacaagtac atcccggcgg tcgaaaaggg cgtggtcgag gcgatggagc
     1861 gcgggccgct gaccggctct caggtgatcg acgtgaaggt ggcggtcttc tacggttcgt
     1921 accacgaagt cgattcgtcg gatatggcat tcaagatcgc ctcctcgatg gccttcaaac
     1981 agggcttcat gcaggccaag ccggttctgc tcgagccgat ctacaacgtc gaggtgctag
     2041 tccccgagga tttcaccggc gacgtcatgg gcgatctctc ggcgcggcgc ggaaagatcg
     2101 ccggcatgga tccggcgggc acgcaccagc ggatccgcgc ccaggtgccg caggcggaga
     2161 tgtaccagta ctcggtcgat ctccgctcga tgacccaggg ccagggcgtg tataccatgg
     2221 agttctccca ctacgaggag gtcccgcacg agaccgccca gaaagtgatt gaggaagcta
     2281 aagccgcggc cgaggaagcc tcgtaagcgc ggcctcggtc tcgtctgctg tccaggctcc
     2341 ggccccggtc ttcccgaccg gggccgtttc tgttggcgcc gggccggccc acaatcacat
     2401 tcttcctgag gtggtttttg ttattgacag ctgaacacat gggcagtata gatgagccaa
     2461 gcaacgcgca acgatgagga agccacatgc cacgagccga tttgacggac aaacagcggg
     2521 cggtgctcgc gtatatcgag aggcagatcg ccgagcgcgg ccactccccc acgatccggg
     2581 agatcgggca gcagttcggc attgcctcga ccaacggcgt ccggacccat ctcaccgcac
     2641 ttattcggaa gggatacctg aagaaggagg cgcagatttc gcgggggctg gcaccgacgc
     2701 gcacgctggc gctccgggtg gggcgggttc cgctggtggg gacggttccg gccggccgcc
     2761 cgatcgacgc ggtcgagaat gtcgagggtg agatcgccgt cgacgagtcg tttcttccga
     2821 aagaggactc gttttcgctg cgggtggccg ggaattcgat gcagggcgcg gggattctcg
     2881 acggcgacat cgtgctggtg aaaaagcagc gggtggcgca gaagggggat atcgtggtgg
     2941 cgattatcgg cggcgaggca accgtcaagc gctatttccc ggaggggaac cgggtacggc
     3001 tgcagccgga aaacgatgac tttgaaccga tttttgtgga tcgcaagtcc gacgagttcc
     3061 ggatcgcggg taaagtcatc gggttgttgc ggcggctgaa gtagcttttc ccggtgcggc
     3121 gcctacggat tttgggacac aagaaagccc gcacggacgc gggcttttcc gtatcaaagt
     3181 cggccgcttg gctcagcctt tggattccgg cttgggcagg accgcctcga tctcaatgat
     3241 gatgtcgacg ttgtcgccgg caacgagtcc gccgttgtcg aggagtttac tccacgagac
     3301 gccgaaatcc tggcggttga tggtcgcggc ggcggagaaa ccggcccgcg tgttgcccat
     3361 gaagtcggtc accacaccat tgagggtgcc gtcgaacgtc acttctttag tcacgccccg
     3421 gatggtcagg tcgccgatga gcttgaactt atccccctca cccgggatca ccttctttga
     3481 cacgaatgtc agctcggggt aggtggcggc gtcgaagaaa tcggcgctgc gcaggtgttc
     3541 atcgcgcttg tcgttgtcgg tgttgatgga ggccgccttg gcggtcatgg acactttggc
     3601 gctggccagc tcgagcttgc cgtcggccgc ctgggccatc tcgaggttgc cgtcgaa
//
LOCUS       DAOPOE010000172         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_28130_length_3593_cov_5.585642, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000172 DAOPOE010000000
VERSION     DAOPOE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28130_length_3593_cov_5.585642"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKM94_13795"
     CDS             <1..446
                     /locus_tag="PKM94_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67750.1"
                     /translation="QTCVNCGRTLVKQKGVWICLACNPTAIDTADIFVDPVESEDYLL
                     IECPGVSLYDPATGEVVEGVRWRDENDATKGFEIVGRPIYAPNHKHRRRVRKEAVGKI
                     RRCRACQDYTIRMRRPEGRDFFIPSVRHPRRTRLRPMEHVTYEPH"
     gene            543..1220
                     /locus_tag="PKM94_13800"
     CDS             543..1220
                     /locus_tag="PKM94_13800"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOD67751.1"
                     /translation="MTRTLLHSALGGLVLLALALPAVAGLFTSRNHRDGSGAIVTEIR
                     DFGAFDRIRSSGSFDVDVTIGPVQKVTVSFDDNLIDLVKTELRGRTLQIYTDGSFSSR
                     KSCRVEITVPALEEAIVSGSGDLVVQGLSGAEFAYDISGSGDATLSGAVDEVRIEVSG
                     SGDVDARDLKARRAYVDIAGSGDVAVWASELFEGVIAGSGDIAYYGNPEKVNRSVAGS
                     GDIRKGE"
     gene            1636..1908
                     /locus_tag="PKM94_13805"
     CDS             1636..1908
                     /locus_tag="PKM94_13805"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOD67752.1"
                     /translation="MDKLVASLGIGKGYYGWLIVANWDKIAGEPIARVARAVRFDDGV
                     LYVAVAGDAWRQELAMQIDALMANIRRLPYGRVVSQVRFVRREKGS"
     gene            1914..>3593
                     /gene="gyrB"
                     /locus_tag="PKM94_13810"
     CDS             1914..>3593
                     /gene="gyrB"
                     /locus_tag="PKM94_13810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOD67753.1"
                     /translation="MPTKVQQVKDDNKANGHSYNATTIKVLEGLEAVRRRPSMYIGDT
                     SQRGLHHMVYEVVDNSVDEAMGGFCDHIIVEIGQDESITVTDNGRGIPIEIHPEQKVS
                     ALQVVMTTLHAGGKFDHDSYKVSGGLHGVGVSVVNALSEECWVEVTRDGEVYRQDYRR
                     GKPVQPVRAVGKRKKSGTRTWFKADPDIFQAVKFKFDIIASRLRELAFLNQGLKIDLV
                     DHRTGKTVTFFAKGGLSEFVDYLNESKTPLFRKPVCFAAAKDDVQVEVAVSYNDTYAE
                     SVYSFVNNINTIEGGTHLTGFRTALTRTINNYAAKNNLVKSNGLSLVGDDSREGLVAV
                     ISVRVRDPQFEGQTKTKLGNSEVRGIVEQITNEYLGMYFDENPAVAKRIVEKIVQAAT
                     AREAARKAKELTRRKSALDSASMPGKLADCSLRDPESTELYIVEGDSAGGSAKQGRDR
                     RFQAILPLRGKILNVEKARIDRILSNNEIRSIITALGCGVGEDFDLGRLRYHKIIVMT
                     DADIDGAHIRTLILTFFFRHMRDLIEHGHVYIAQPPLYRLKHAKSEQYAYSD"
BASE COUNT          701 a         1271 c         1029 g          592 t
ORIGIN      
        1 cccaaacctg cgtcaactgc gggcgcaccc tggtcaaaca gaaaggcgtc tggatctgcc
       61 tggcctgcaa tcccaccgcc atcgataccg ccgacatctt cgtcgatccc gtcgaaagcg
      121 aggactacct cctgatcgag tgccccggcg tgtctctcta tgacccggcg accggcgagg
      181 tggtcgaggg tgtccgctgg cgcgacgaga acgacgcgac caaggggttt gaaatagtcg
      241 gccgcccgat ctatgctccc aaccacaagc accgtcggcg cgtccgcaag gaggcggtgg
      301 gaaaaatccg ccgctgtcgc gcctgccagg actacaccat ccgcatgcgc cgccccgagg
      361 ggcgcgactt cttcattccc tcggtccgcc acccgcgccg cacccggctc cgccccatgg
      421 aacacgtgac ctacgagccc cactgacccg gcgatttgtg cgccgccggc gaaacctgcg
      481 cccgccggcc ccgtaactcc aggtaggcaa gaatacaacc gtccactacc tggagagagg
      541 caatgaccag aactttgctc cactcggccc tcggcggctt ggtgctcctc gccctggccc
      601 tgcccgccgt cgccggcctg ttcaccagcc gcaatcatcg cgacggctcc ggcgccatcg
      661 tcaccgagat ccgcgatttc ggcgccttcg accgtatccg cagttccggc tcgttcgatg
      721 tcgacgtgac tatcggcccc gtccaaaagg tgaccgtcag cttcgatgat aacctcatcg
      781 acctcgtcaa gacggaactc cggggccgca cgctgcagat atacaccgac gggtcgttca
      841 gctcccgcaa atcctgccgc gtggaaatca ccgttcccgc tcttgaggaa gccatcgtct
      901 cgggctccgg cgacctggtc gtccagggac tcagcggcgc cgaattcgcc tatgacatct
      961 cgggctccgg cgacgcgact ctcagcggcg cggtcgacga agtgcggatc gaggtgtccg
     1021 gttccggcga tgtcgatgcc cgcgacctga aggcccggcg cgcctatgtc gatatcgcgg
     1081 gctccggcga cgtcgctgtc tgggcgagcg agctgttcga aggcgtcatc gccggcagcg
     1141 gcgacatcgc ctactacgga aaccccgaga aagtgaatcg cagcgtggcc ggcagcggcg
     1201 acatcaggaa aggggagtag ccctttccga gcaccggtcg cggacggacg atcggtttga
     1261 agacatcccc gccgatggtg gacctcccct cggttgggcc gttcccttca cataaccgcc
     1321 gacgctcggc gggagggcaa gtcctcccgc cgttgctttt ggtgtgtgcg ctgttcggcc
     1381 ttcccggttc ggcgccgcct gcttttccac tttttgcccg ctcggccggg ccggaaaccg
     1441 gcggccgcaa gggcggccca tttgtcgccg caagttgctg tacaataaga ggttattagc
     1501 ctctttttgc ttgcgttcgg atttgaactt ttgtatctta aggggttaca aaatatgtgt
     1561 gccgcaccgc ccgaaaaacg cgacgaaccg ggacaatatc ggtcccacaa caccccgaaa
     1621 ccaatcggcg gggtgatgga caaactcgtc gcctcgctcg gcatcggcaa gggctactac
     1681 ggctggctca tcgtcgccaa ctgggacaag atcgccggcg aaccgatcgc ccgcgtcgcc
     1741 cgggcggtgc gctttgacga cggcgtgctc tacgtggcgg tcgccggcga cgcctggcgg
     1801 caggaactgg ccatgcagat cgacgctctc atggccaaca ttcgccgcct cccctacggc
     1861 cgggtggtct cgcaagtgcg gttcgtacga cgtgagaaag gaagctagga catatgccga
     1921 ccaaggtgca gcaggtgaaa gacgataaca aggccaacgg ccactcttac aacgccacga
     1981 cgatcaaggt ccttgaggga ctcgaggccg tgcggcgccg cccctcgatg tacatcggcg
     2041 acaccagcca gcgcggcctc caccacatgg tctacgaagt cgtcgacaac tcggtcgacg
     2101 aggccatggg cggcttctgc gaccacatta tcgtcgaaat cggccaggac gaatcgatca
     2161 ccgtcaccga caacggccgc ggcatcccca tcgaaataca ccccgagcaa aaagtctccg
     2221 cccttcaggt ggtcatgacc accctccacg ccggcggcaa attcgaccac gatagctaca
     2281 aggtctcggg cggcctccac ggcgtcggcg tctccgtcgt caacgccctc tccgaagagt
     2341 gctgggtcga ggtgacccgc gacggcgagg tgtaccgcca ggactaccgc cgcggcaaac
     2401 ccgtccagcc ggtcagggcg gtcggcaagc gcaagaagag cggcacccgc acctggttca
     2461 aagcggaccc ggacatcttc caggcggtga aattcaaatt cgatatcatc gcctcccgcc
     2521 tgcgcgaact ggccttcctc aaccaggggc tcaagatcga tctcgtcgac caccgcacgg
     2581 gcaaaaccgt caccttcttc gccaagggcg ggctctccga attcgtcgac tacctcaacg
     2641 agagcaagac gccgctgttc cggaaacccg tctgtttcgc cgccgccaag gatgacgtcc
     2701 aggtcgaggt ggccgtctcc tacaacgaca cctacgccga gtcggtctac tcgttcgtca
     2761 acaacatcaa caccatcgaa ggcggcaccc acctcaccgg tttccgcacc gccctcaccc
     2821 ggaccattaa caactacgcc gccaagaaca atctcgtcaa gtcgaacggc ctgtcgctcg
     2881 tgggcgacga cagccgcgag gggctggtgg cggtcatctc ggtgcgcgtg cgcgatcccc
     2941 agttcgaggg ccagaccaag accaagctcg gtaacagcga ggtccgcggt atcgtcgagc
     3001 agatcaccaa cgagtacctc gggatgtact tcgacgagaa cccggccgtg gccaagcgca
     3061 tcgtcgaaaa gatcgtccag gcggccaccg cccgcgaggc cgcccgcaaa gcgaaggaac
     3121 tcacccgccg caagagcgcc ctcgactcgg cctccatgcc cggcaaactt gccgactgct
     3181 cgctccgcga ccccgaatcg acggagctgt acatcgtgga gggcgactcg gccggcggtt
     3241 cggccaaaca ggggcgcgac cgccgcttcc aggccatcct cccgctgcgc ggcaaaatcc
     3301 tcaacgtcga aaaagcccgc atcgaccgga tcctctccaa caacgagatc cgctcgatca
     3361 tcaccgccct cggctgcggc gtcggcgagg atttcgacct cggccgcctg cgctaccaca
     3421 agatcatcgt catgaccgat gccgacatcg atggcgccca tatccgcacc ctcatcctca
     3481 ccttcttctt ccgccacatg cgcgacctca tcgagcacgg ccacgtctac attgcccagc
     3541 cgcccctcta ccggctcaaa cacgccaaga gcgagcagta cgcctacagc gac
//
LOCUS       DAOPOE010000173         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_28971_length_3514_cov_4.524140, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000173 DAOPOE010000000
VERSION     DAOPOE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28971_length_3514_cov_4.524140"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKM94_13815"
     CDS             <1..703
                     /locus_tag="PKM94_13815"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481823.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOD67754.1"
                     /translation="LGVSRPGDMGFDVMHFNLHKTFSTPHGGGGPGAGAVGVTAELDP
                     FLPFPVLEKADDGTLFMNYNRPHSIGRVHAFYGNFANQVRAYAYIKTLGGRGLRRASE
                     NAVLNANYLKALLKDAFDLPYDVHCMHEFVLSGNRQKKKGVRTADMSKRMLDFGVHSP
                     TNYFPLIVPEALMIEPTETESRETLDRFAAIMRQIAREADTDPELVTSAPHHTPVRRL
                     DETAAARTLDVCFAE"
     gene            759..2240
                     /locus_tag="PKM94_13820"
     CDS             759..2240
                     /locus_tag="PKM94_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD67755.1"
                     /translation="MVRFEGVTYRYGAGAPAALANVGALIQPGESVAVMGANGSGKST
                     FARLAAGLFAPTEGRVTVRNQRGEDTRAGILFQNPDNQMVAVTVEKEIAFALENAAVP
                     PDVMARRVTQTLKRFSIAHLRQRLTAELSGGEKQRVALAAVMVSEPTVLVLDEPDSFL
                     DEAGKAMLRTELENLRRSQPSMIQIHITQYPQTARQYPRLMVFFEGRVAADGRPERIF
                     ADDAFCRETAIAFDPRASDGEGSGAASIAAKDRRVARVELKEAAFAYAAIGPVLPPLN
                     ATVQSGEVLGVVGPSGSGKSTLGLLLCRVLEPTSGRILYIGPEGGPVFPESAPGRVSA
                     ILQQPERQFFLPTCEQEVEFGPENIGRPLTITETHAMLRLAGLDPTVFGKRDPFRLSG
                     GEKRRLAFAAVLSLRPDIVVFDEPTCGLDQEGVGRFIRLARTLRAGGAGVVVISHDGD
                     VIRALADRVLVLGRDREYRLIGADEFFAAAELRRAVSPVTWAG"
     gene            2368..3012
                     /locus_tag="PKM94_13825"
     CDS             2368..3012
                     /locus_tag="PKM94_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOD67756.1"
                     /translation="MEASGIKADTGKEQAQTGSGETAARVIRDTTSNPAPLGLLGFGM
                     TTVLLNLHNAGIFPLDAMILGMGIFYGGMAQVFAGIMEWKKGNTFGTTAFTSYGLFWL
                     TLVALLVLPKLGWGAAPEAIAMAAYLAMWGIFTAVMFVGTLRLNRALQFVFASLAVLF
                     FLLAIGDATGSAGIKTLAGIEGIICGLSAVYAGLAQVLNEVYGRTLWPLGAVAK"
     gene            3168..>3514
                     /locus_tag="PKM94_13830"
     CDS             3168..>3514
                     /locus_tag="PKM94_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67757.1"
                     /translation="MKKVMLAAALLALPAASLCGQDFGIKFSGFVKSDFFHDSRQTVS
                     LREGHFLLYPAGISRDVRGEDVNDRGSFNYLAIQTRLTGAITAPDAFGAKVSGVLEAD
                     FFGNESAALVDANG"
BASE COUNT          573 a         1057 c         1283 g          601 t
ORIGIN      
        1 gctcggcgtc tcgcgccccg gcgacatggg cttcgacgtc atgcacttca acctgcacaa
       61 gacgttctcg acgccgcacg gcggcggcgg gccgggcgcc ggagcggtcg gggtcaccgc
      121 ggaactcgac ccgttcctcc ccttcccggt gctggaaaag gcggacgacg ggacgctgtt
      181 catgaactac aaccgcccgc actcgatcgg gcgggtgcac gcgttctacg ggaatttcgc
      241 caaccaggtg cgggcctatg cctacatcaa gacgctcggc ggccggggac tgcggcgcgc
      301 cagcgagaac gcggtgctca acgccaacta cctcaaggcg ctgctgaagg atgctttcga
      361 tctgccctac gatgtccact gcatgcacga attcgtgctg tcgggaaacc ggcagaagaa
      421 gaaaggggtg cggacagcgg acatgtcgaa gcggatgctc gatttcggcg tgcactcgcc
      481 gaccaactat ttcccgctca tcgtgccgga ggcgctcatg atcgagccga cggagacgga
      541 gagcagggag acgctcgacc ggtttgcggc aatcatgcgc caaatcgcgc gcgaggcgga
      601 caccgacccc gagctcgtga cttcggcgcc gcaccacacc ccggtgcgcc ggctggacga
      661 gacggcggcg gcgcggacgc ttgatgtctg ttttgcggag tgaacgattc cgcttgtgaa
      721 tgggagccgg tgcctgttct ttgggcatcg gcttttttat ggttcgtttc gaaggtgtga
      781 catatcgcta cggcgcgggg gcgccggcgg ccctcgccaa cgtcggggcg ctcattcagc
      841 ccggcgagtc ggtggccgtc atgggggcca acgggtcggg gaaatcgacc ttcgcccgcc
      901 tcgccgcggg cctgtttgcg ccgaccgagg gacgggtgac ggtgcggaac cagcgcgggg
      961 aagacaccag ggcggggatc ctctttcaga atcccgacaa ccagatggtg gcggtgacgg
     1021 tcgagaagga gatcgccttc gccctcgaaa acgcggccgt gccgcccgac gtcatggccc
     1081 ggcgggtgac ccagacgctc aagcgcttct cgatcgccca tctccggcag cgcctgaccg
     1141 cggagctgtc gggcggtgaa aagcagcggg tggccctggc ggcggtcatg gtgagcgagc
     1201 cgactgtgct cgtgctcgac gagccggatt cgtttctcga cgaagcgggg aaagcgatgc
     1261 tgcggaccga gctggagaac ctgcggcgga gccagccctc gatgatccag attcacatca
     1321 cgcagtatcc gcagacggcg cggcagtacc cgcgcctgat ggtcttcttc gagggccgcg
     1381 tggcggccga cggccggccg gagcgaatct tcgccgacga tgccttctgc cgcgaaacgg
     1441 ccatcgcctt cgacccgcgc gccagcgacg gagagggcag cggcgcggcg agtatcgcag
     1501 cgaaggaccg ccgggtggcg cgcgtcgaac tcaaggaggc ggcgttcgcc tatgccgcga
     1561 tcggaccggt gctgccgccg ctcaacgcga ccgtccagtc cggcgaggtg ctcggggtcg
     1621 tcggaccctc gggatcggga aagagcacgc tcgggcttct tttgtgccgg gtgctggaac
     1681 cgacctcagg aaggatcctc tacatcggcc cggaaggcgg gccggtgttt ccggagagcg
     1741 cgccggggcg ggtgagcgcg atcctccagc agccggagcg gcagttcttc ctcccgacct
     1801 gcgaacagga agtcgagttc ggccccgaga atatcgggcg gccgctgacg atcacggaga
     1861 cacacgccat gctgcggctg gccggactgg atccgaccgt gttcggaaag cgggatccgt
     1921 tccggctgtc gggcggagag aaacggcggc tggcgttcgc cgcggtgctg tcgctgcgcc
     1981 cggatattgt cgtgttcgac gagccgacct gcgggctcga ccaggaaggg gtggggcggt
     2041 tcatccggct ggcgcggacg ctgcgcgcgg ggggagcggg ggtggtggtg atcagccacg
     2101 acggcgacgt catccgggcg ctcgccgacc gcgtgctggt cctcggccgg gatcgggaat
     2161 accggttgat cggagcggac gagttctttg cagcggccga gctgcgccgg gcggtgtcgc
     2221 cggtcacgtg ggcgggttag cgcccggcca ggcggccccg ggccggcccc gctctcattt
     2281 tgcagttgat gaacgcgggg cgcattgtct atatttgcgc gtggtgcagg tgagaggttg
     2341 gcaacgactc taacaggatt ggtcgacatg gaagcatcag ggatcaaggc ggacacgggc
     2401 aaagaacagg cgcagacggg gagcggcgaa accgcggccc gggtgatcag ggacaccacc
     2461 tccaacccgg ctcccctcgg gctgctcgga ttcggcatga cgacggtgct gctgaatctc
     2521 cataacgccg gaatctttcc gctcgatgcg atgattctcg ggatggggat attttacggc
     2581 gggatggcgc aggtgttcgc aggcatcatg gagtggaaga agggaaatac gttcggaacg
     2641 acggcgttta cgtcatacgg gctgttctgg ctcacgctcg tggctctgct cgtgctgccg
     2701 aagctgggct ggggcgcggc gccggaggcg atcgcgatgg cggcctatct ggcgatgtgg
     2761 ggaatcttta cggcggtgat gtttgtcgga acgctccggc tcaaccgcgc cctccagttc
     2821 gtgttcgcct cgctcgctgt gctgttcttc ctgctggcga tcggcgacgc gacgggcagc
     2881 gcgggcatca agacgctggc cggaatcgaa ggaatcatct gcgggttgtc ggcggtgtac
     2941 gccggcctgg cgcaggtgct caatgaggtg tacggccgga cattgtggcc gctgggggcg
     3001 gttgcgaagt gacgaaccgg atatccggag acggtcgggg cgggatggtt ccgccccgct
     3061 tttttgtcgg cgctcaggag gctcccgcgc tcaggcggcg cggcagagcg ctccgcgaac
     3121 aaccaatgga tccccggcgg ggagtcgggg aagcggcggg gtggttcatg aagaaggtta
     3181 tgcttgcggc agcgctcctg gcgctgccgg cggccagcct ctgcggccag gatttcggca
     3241 tcaagttctc gggctttgtg aagagcgatt tctttcacga ttcgcggcaa acggtcagtc
     3301 tgcgcgaggg gcatttcctg ctgtacccgg ccggaatcag ccgggacgtc cggggcgagg
     3361 atgtcaacga tcgggggagt ttcaattacc tggcgatcca gacgcggctc accggggcaa
     3421 tcacggctcc cgatgctttc ggggccaaag tgagcggcgt tctggaggcc gatttcttcg
     3481 gcaacgagag cgcggcgctc gtggatgcca acgg
//
LOCUS       DAOPOE010000174         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_30378_length_3390_cov_3.287856, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000174 DAOPOE010000000
VERSION     DAOPOE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30378_length_3390_cov_3.287856"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="PKM94_13835"
     CDS             <1..1719
                     /locus_tag="PKM94_13835"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOD67758.1"
                     /translation="LLVTDEEYCGPLLQAIFERDNFDVTTTDSADDAIEAIGNRRFHS
                     VFIKDTVPGDYLDLIDRLRKISPRTRVRYYESAARLLLVDDLFTTEGDLLVKNLDLFT
                     AMMSTQARQPDNHSGRVGHYADRLCRRLGLPDKDRLQIVSAAYLHDLARFHYTPSETR
                     DTRTTIKLTTRLLQSLNYPPVVVEMLRKMYVDLKGKYTKRLPIEALGGNILTIVDLYC
                     ENVAANERLLLDKFDAIKKKFRDLTGRLFMAEVAEAFIGLIQEELLAQQTSLQVAQVM
                     LLSDRPGSLTPAYLRLRNEGFRTMAPAGLDSFVDLYRRSRPDIIVLAVSGSAEDVVAF
                     IERLRQRDVDFDHTPAFALVDSAVVPSLTGLLEKGIEDILPVDAGLDLLVVKMQKIQA
                     VLQAKQKQRETADHKSGATGRLADMNLIDLLQALGPSRRTARITVTPGPSAARADDSR
                     PGLEPDPRGGFSDNSRAGRSGDAAGAGSTPGRPDRRPDQKDRRLNDHLILYLDRGAIV
                     FAQYRDKIGAEAVYETIGWADGYWSVEPVSREALPAPNNDLSNESILMEGCRLLDERL
                     HAGKLF"
     gene            complement(1911..2960)
                     /locus_tag="PKM94_13840"
     CDS             complement(1911..2960)
                     /locus_tag="PKM94_13840"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HOD67759.1"
                     /translation="MTVRLTYSRRATAAVVAVVLAALLPAHTSARAAAYPDSQQVRLL
                     AALQEDLYNDRYDAAEKVARLMVSSYPDDPLGHLFTGITLVTAAYDAEEALREREFRT
                     SLAAAERLAGLQIASGDRHTRAWGFLVLGHAASYRAVWEAKFGSKLTAARAGRTAAGL
                     YERGLRADSTVYDLYFGLGLYHYWKSAKAGVLRWLGIVSDDKERGLRELETAAEGSLI
                     SREAARSSLVWVRLDRKEYREAVALGDSLMRRYPHSRSFLWPVAEAQYRLGRYADAAA
                     AYGRLREMLREEPGNYYNLIECDYRIARCFEMMGLPGEWAGTAADVSGYMGEVSEETQ
                     ERQRERLEYLGVAGE"
     gene            3155..>3390
                     /locus_tag="PKM94_13845"
     CDS             3155..>3390
                     /locus_tag="PKM94_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67760.1"
                     /translation="MVTGVLVDGWPVGPVSAYTFYNVTGNHTIVASFAADPGATVVAA
                     SDYPHPEDAAYVCDGTDDQVEIQAAIDALGGSPGR"
BASE COUNT          567 a         1282 c          964 g          577 t
ORIGIN      
        1 ctcctcgtca ccgacgagga gtactgcgga ccgctccttc aggcgatctt cgaacgcgac
       61 aatttcgacg tgaccacgac cgattccgcc gacgatgcga ttgaggcgat cggcaaccgc
      121 cgcttccaca gcgtcttcat caaagacacc gtccccggcg attacctcga cctgatcgac
      181 aggctccgca agatctcgcc gcgcacccgg gtgcgctact acgaatcggc cgcccgcctg
      241 ctcctggtcg acgatctctt cacgaccgag ggggacctcc tcgtcaagaa cctcgacctt
      301 ttcacggcca tgatgtcgac gcaggcgcgc cagcccgaca accacagcgg ccgcgtcggc
      361 cactacgccg accgcctctg ccgccgcctc ggcctgcccg acaaagaccg cctccagatt
      421 gtctcggccg cctacctcca cgacctcgcc cgcttccact acaccccgtc cgagacccgc
      481 gacacccgca cgaccatcaa actcaccacc cgtctcctcc agtcgctgaa ctacccgccg
      541 gtggtcgttg aaatgctgcg gaaaatgtac gtcgacctca agggcaagta caccaagcgc
      601 ctccccatcg aggccctcgg cggcaacatc ctgaccatcg tcgacctcta ctgcgaaaat
      661 gtcgccgcca acgagcgcct cctcctggac aaattcgacg cgatcaagaa gaaattccgc
      721 gacctcaccg gccgcctctt catggccgaa gtcgcggagg ccttcatcgg cctcatccag
      781 gaggaactgc tggcgcagca gacctctctg caggtcgccc aggtgatgct gctgtccgat
      841 cggcccggct ctctcacccc cgcttacctc cgcctgcgca acgagggttt ccgcaccatg
      901 gcccctgccg gcctcgactc cttcgtcgat ctctaccgcc gcagccgccc cgacatcatt
      961 gtcctcgcgg tctccgggtc ggccgaggac gtcgtcgctt tcatcgagcg cctgcgccaa
     1021 cgcgacgttg atttcgatca caccccagcc tttgctttgg tcgacagcgc cgttgtcccc
     1081 tcgctcaccg gactcctgga gaaggggatc gaggacattc ttcccgtcga tgccggcctc
     1141 gacctgctgg tggtgaagat gcagaagatc caggcggtgc tgcaggcgaa gcagaagcag
     1201 cgcgaaaccg ccgaccacaa gtccggcgcc accggccgtc tcgccgacat gaatctcatc
     1261 gacctcctgc aggccctcgg cccgagccgc aggacggccc gcatcaccgt cacccccggc
     1321 ccctccgccg ctcgagccga cgattctcgc ccgggccttg agcccgaccc gcgcggcggt
     1381 ttctccgaca attcgcgcgc cggccgttcc ggcgacgccg ccggcgccgg aagcactccc
     1441 ggccgccccg atcgccgccc cgaccagaaa gaccgccgcc tgaacgacca tctcatcctc
     1501 tacctcgacc gcggagcgat cgtcttcgcc cagtaccggg acaaaatcgg cgccgaggcg
     1561 gtctatgaaa caatcggctg ggcggatggg tactggtcgg tggaaccggt gtcccgcgag
     1621 gcccttcccg ctccgaacaa cgacctctcc aatgagtcga ttctcatgga aggctgccgc
     1681 ctcctcgacg aacgcctcca cgccggcaag ttgttttgac ctttcctccg atggcgccgg
     1741 gcacacaggt aacagccgtc ccccggatct cttgtgggtc aggacccttg tggtcctgac
     1801 attctgacca tcgatgtgag cagccccgtc aggatcataa aggtcctgac ttacaagacc
     1861 tatttcgcgg aggttattcg gcccgtgtcg cgatcgcctt caccggcggc ttactccccg
     1921 gccaccccca gatactccag ccgctcccgc tgccgctcct gcgtctcttc cgacacctct
     1981 cccatgtatc ccgacacatc ggcggccgtg cccgcccatt cgcccggcag gcccatcatc
     2041 tcaaagcacc gggcgatccg atagtcgcac tcgatcaggt tgtaatagtt gccgggctct
     2101 tcgcgcagca tttcccgcaa ccggccgtac gccgccgccg catcggcata gcgtcccagg
     2161 cgatactgcg cctcggccac cggccacagg aagctccggc tgtgcgggta gcggcgcatc
     2221 aggctgtcgc cgagcgcaac cgcttcccgg tactccttac gatccagccg cacccagacc
     2281 agcgatgatc tcgcggcctc cctcgaaatg agcgacccct ccgccgccgt ctccagctcc
     2341 cgcaggcccc gttccttgtc atcggataca attcccagcc atcgcagtac tcccgccttc
     2401 gccgacttcc agtaatggta cagccccaac ccaaagtaca ggtcgtacac cgtgctgtcg
     2461 gcccgcagtc cccgttcgta gagtccggcc gcggttctcc cggcgcgcgc ggcggtgagc
     2521 tttgatccga acttggcttc ccagaccgcg cggtaggagg cggcgtgtcc gagcacgagg
     2581 aatccccagg cccgcgtgtg ccgatctccc gacgcgatct gtaatcccgc caagcgctcc
     2641 gccgcggcga ggcttgtgcg gaactcgcgt tcgcgcagcg cctcctcggc gtcgtaggcc
     2701 gccgtgacca gcgtgatgcc cgtgaacagg tgtcccagcg ggtcatccgg gtacgaggac
     2761 accataagtc gcgcgacctt ctcggctgcg tcgtaccggt cgttgtagag gtcttcctga
     2821 agcgcggcca gcagccgcac ctgctgggaa tccgggtacg cggctgcccg tgcgctcgtg
     2881 tgcgcgggga ggagcgccgc caggactacg gcgacgaccg cggccgtcgc acgccgggag
     2941 tatgtgagcc ggaccgtcac atgcctagta tcggccaata gggtcggcga ttgagcgggg
     3001 gaaaagttgg cagctcggcc gggaggcggc cggggccatc agctatacca tcaccagcgg
     3061 ttccggcgac tgcggctctg tttccccctc cggccccgtg agcgctcccg ccggctccac
     3121 gcagacgttt cgcatcatcc cgggggcctc ctgcatggtg accggggtcc tggtggacgg
     3181 ctggccagtg ggcccggtga gcgcctacac cttctacaac gtgaccggca accacaccat
     3241 tgtggccagc ttcgcggctg atcccggcgc cacggtggtg gccgcctccg attatcccca
     3301 cccggaggac gccgcctacg tctgcgacgg gaccgacgac caggtggaga tacaggccgc
     3361 cattgacgct ctgggcggat cgccggggcg
//
LOCUS       DAOPOE010000175         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_30717_length_3361_cov_4.777072, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000175 DAOPOE010000000
VERSION     DAOPOE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30717_length_3361_cov_4.777072"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKM94_13850"
     CDS             <1..795
                     /locus_tag="PKM94_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOD67761.1"
                     /translation="EAIISVFDNSLQYAEGLFEMLLAVDDRPIFPRDHLRRLQRGSRV
                     IGLELPVTPAQLERWMVKTLRAHPARIKKLRLTITAGESARWFGRPGRPQVILSAGPH
                     RMPTVPFRLWVSDFRVDQNSILHRIKTLSYALHAAAFRQAHRRRCDDALLLNERDEVA
                     EVTSANIFWVDRGTIYTPPLSSGCLDGVTRRIVLRECRRLKLPVAERPAFLRDLCAAD
                     EVFISSSTKLVIGVGGISGPGGGCTIPQGPVTGQLFRHFRKLVGLD"
     gene            897..1739
                     /locus_tag="PKM94_13855"
     CDS             897..1739
                     /locus_tag="PKM94_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67762.1"
                     /translation="MKVRFVCLSAVILSVGLCALARPAAAQPQEPVQRVPHGSTSLYE
                     LPPRWLVDIPTAGTLPRGYWDVVIRLSPYGAATGYIDIGLSNRFQLGLSYGGIDIISA
                     SSPDWNPDMGINIKFRVVDELEYFPAVALGFASQGSGPYNDDLKRYTFKSRGFYGIVS
                     RSFYFYQWTAGGHFGVNYTLENDVDDESDVNFFAGFDATFKYNLAFFAEWDAALNDDA
                     SALPGGEPYTFAGRGRGYLSMAVKWLFTDNLQLEFIAKDLTLNRRESDTFSREVRITY
                     IDRF"
     gene            complement(1770..2504)
                     /locus_tag="PKM94_13860"
     CDS             complement(1770..2504)
                     /locus_tag="PKM94_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOD67763.1"
                     /translation="MERRLQRLSWLRIRELVPAEIDTAVLPVGTVEAHGAACLGTDIY
                     IPEDIAGHIAERLNALVAPTVTYGITKSLLRYNGGATVRPETFGAYVRDVLDSLAAAG
                     FRHVIVINGHGGNNAVLKDVAYAFHAERRANIAVIHWWQLCADMTENFFGHIGGHAGT
                     DETAYIWAMDPALADRERYDPDLAYTFNPGADVYPVPGTILLYKAGEGYPEFDDRRAK
                     EYREKVIDAVGEFAAMVLGRWRKFGL"
     gene            complement(2508..3251)
                     /locus_tag="PKM94_13865"
     CDS             complement(2508..3251)
                     /locus_tag="PKM94_13865"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HOD67764.1"
                     /translation="MARTKKNDSVIAVCVQEPTEDGSQMELGAIQGDSLRFLHQAFIT
                     DTINHALDADVDVRLYYAELPERERLVGIITEYLRKKSSGKRATALKNRFQGVKLPME
                     RWGLRIEKVFQDAFAAGYRHVLVIGSRTPTVKTDQMCRALRMLRESDAVFGPTPEGRY
                     YVIGMSSEYRVKLSEFDWKSPSIYHDVSNAFTEQELRWAELEIWYCVEDPDELELMVR
                     DINQFRFEGDELSARETELVIERLLSRME"
BASE COUNT          547 a         1182 c         1004 g          628 t
ORIGIN      
        1 gaggccataa tctcggtctt cgacaactcg ctgcagtacg ccgaggggct gtttgagatg
       61 ttgctggcgg tggacgatcg gccaattttc ccccgcgatc acctccgccg cctccagcgg
      121 ggatcgcggg ttatcgggct cgagctgccg gtcacaccgg ctcagcttga gcgctggatg
      181 gtcaagacgc tgcgggccca tccggcgcgc atcaagaaac tccgcctgac catcaccgcc
      241 ggcgaatcag cccgctggtt cggccggccc ggccggccgc aggtgatcct ctcggccggt
      301 ccgcaccgga tgccgaccgt cccgttccgt ctctgggtct ccgatttccg agtcgaccaa
      361 aattcgatcc tccacaggat caagacgctc tcctacgcac tccacgccgc cgccttccgc
      421 caggcgcacc gtcggcggtg cgacgacgcc ctcctgctca acgagcgcga cgaagttgcc
      481 gaggtcactt cggccaacat cttctgggtc gaccgcggaa ccatctacac gccgccgctc
      541 tcgtcaggct gtctcgacgg cgtcacgcgc cgcatcgtgc tgcgcgaatg ccgccgcctg
      601 aaactccccg tcgccgagcg acccgccttc ctgagagacc tctgcgccgc cgacgaggtc
      661 ttcatttcca gttccaccaa gctcgtgatc ggggtcggcg gcatcagcgg gccgggcggc
      721 ggctgcacaa tcccccaggg acccgtcacc gggcagctct tccgtcactt ccgcaaactg
      781 gtcggccttg actgaccggc gggcgcggcg cctccgatgt ttttgttgct ttagcccccc
      841 tgccggagct attgcttgcg gtaactgatc attcggagag caatcgaggc cggtgtatga
      901 aagtccgctt tgtctgtctg tccgctgtta tcctgtcagt cgggctgtgc gcgctggctc
      961 ggcccgcggc cgcgcaaccc caggagcccg tccagcgcgt cccgcacggc agcacgagcc
     1021 tgtacgaact gccgccccgc tggctggtcg acatccccac cgccggcacc ctcccccgcg
     1081 gttactggga cgtcgtcatc cgcctctccc cgtacggtgc ggcgaccggc tacatcgata
     1141 tcggcctctc caaccgcttc cagctcgggc tttcctacgg aggcatcgac atcatctcgg
     1201 cctccagtcc cgactggaac cccgacatgg gcatcaacat caagttccgc gtggtcgatg
     1261 agctcgagta cttccccgcc gtcgccctcg gcttcgcctc gcaggggagc gggccgtaca
     1321 acgacgatct caagcggtac acgttcaagt cgcgcggctt ctacggcatc gtcagccgca
     1381 gcttctactt ctaccagtgg acggcgggcg gccatttcgg cgtcaactac acgctggaga
     1441 acgacgtcga cgatgaatcg gacgtcaact tcttcgccgg cttcgacgcc acattcaagt
     1501 acaacctcgc gttcttcgcc gagtgggacg cggcgctcaa tgacgacgcg agcgcgctcc
     1561 ccggcggcga gccctatacg ttcgccggcc gcggccgcgg ctacctgagc atggcggtga
     1621 agtggctctt caccgataat ctgcagctgg agtttatcgc caaggacctg acgctcaatc
     1681 gccgcgaatc cgacaccttc tcccgtgaag tgcggatcac gtatatcgat cggttttgat
     1741 ggcgcacgcg gggccggcgc cggccgcccc tacagcccga actttcgcca ccgcccgagc
     1801 accatcgccg cgaactcccc caccgcgtcg atcactttct cccggtattc cttcgcccgc
     1861 cggtcatcga actccgggta cccctccccc gccttgtaca ggaggatggt ccccggcacc
     1921 gggtagacat cggcccccgg attaaacgtg taggcgagat ccggatcgta ccgctcccgg
     1981 tcggccaggg cgggatccat cgcccagatg tacgccgtct cgtcggtccc ggcgtgcccg
     2041 ccgatgtggc caaagaaatt ctcggtcata tccgcgcaca gctgccacca gtggatgacc
     2101 gcgatgttcg cccggcgctc ggcgtggaag gcgtacgcga cgtctttcag gacggcgtta
     2161 ttgccgccgt ggccgttgat gacgatgacg tggcggaagc cggcggcggc cagcgagtcg
     2221 agcacgtcgc gaacgtaggc gccgaaggtc tccgggcgga cggttgcgcc gccgttgtag
     2281 cgcaggaggg acttcgtaat gccgtaagtg accgtcggcg ccaccagtgc gttgaggcgc
     2341 tcggcgatat ggccggcgat gtcctcgggg atgtagatat cggtgccgag gcaggccgcg
     2401 ccgtgcgcct ccaccgtccc caccggcagc acggccgtgt caatctccgc cggcaccagt
     2461 tccctgatgc gcagccagct caggcgctgc aggcgtcgtt ccaccactca ctccatccgc
     2521 gaaagcaggc gctcgatcac cagctccgtc tcgcgggccg acagctcgtc tccctcgaag
     2581 cgaaactggt tgatgtcccg caccatgagt tccagttcgt cgggatcctc gacgcagtac
     2641 cagatttcca gctccgccca gcgcagctcc tgctcggtga acgcgttcga cacgtcgtgg
     2701 tagatcgacg gcgatttcca gtcgaactcg gacagcttca cccggtactc cgacgacatt
     2761 ccgatcacgt agtatcgccc ctcgggggtg ggcccgaaca cggcgtccga ctcccgcagc
     2821 atgcgcaggg cgcggcacat ctggtcggtc ttgaccgtgg gggtccggct gccgatcacg
     2881 agcacgtgcc tgtagccggc cgcgaaggcg tcctgaaaca ccttctcgat tcgcaggccc
     2941 caccgctcca tcggcagctt caccccctgg aaccgatttt tcagcgcggt cgcccgcttg
     3001 ccgctcgatt tcttcctgag gtactccgtt atgatcccga ccaatcgctc gcgctccggc
     3061 agttcggcat agtacaggcg cacgtcgaca tcggcatcga gagcgtggtt gatcgtgtcg
     3121 gtaatgaaag cctggtggag aaagcgcagg gaatcgccct ggatggcgcc gagctccatc
     3181 tgcgatccgt cttcagtcgg ctcctggacg cacacggcga tgactgaatc gttcttcttt
     3241 gtccgggcca tacgtccctt acgagttagg gtcctctgtc acgccggaat atacatgccg
     3301 ggattcggcg gcgtcaaggt tcgccgaaag agaatttccg gaaaagcggt cggggagagc
     3361 g
//
LOCUS       DAOPOE010000176         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_31465_length_3302_cov_5.420388, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000176 DAOPOE010000000
VERSION     DAOPOE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31465_length_3302_cov_5.420388"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(569..1264)
                     /locus_tag="PKM94_13870"
     CDS             complement(569..1264)
                     /locus_tag="PKM94_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67765.1"
                     /translation="MKFALGLEQTEPGKWIAWVVDLPGCFARASARDEVVRCAPRAIA
                     DYFNWRDGYERVSSEAIEQMGIEVVEEFRNYETLDGYWVNAFFAWDRRPLLAGEIDDV
                     RWLLSCTRSDLISAVRRVPAAALDQPIAGERFGTIAGILTHAATAERWYLDKLGLAPQ
                     KETLGTDPFAMLESVRRHLLMHLPRFANDATVSEGHGEFWSARKVARRALWHERVHTW
                     HIQRLTAQLVNQR"
     gene            1851..>3302
                     /locus_tag="PKM94_13875"
     CDS             1851..>3302
                     /locus_tag="PKM94_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67766.1"
                     /translation="MKHSGIPRIPMLFVLTLICSAGAWAQAEAATIATDSVALCFQEA
                     LFDTIAVDLPNPGNPGVAMALVSGPGALTYSVSDKLYGYYEYAPSGDTAFEIVYRVWY
                     APTDSADFRHRYIVWVDEPPTLGDQYNAFKVCWPGPHYLKVYAWDPESRPLAWEIVSG
                     PGTIDSQGLITYTPDTAGVYVFEVAASDDCNRAAATVYDTVRFNGAPRIVLADSIFSL
                     CAEGEICFDVVFSDPDGDAVWFNQVGGPGSTTVVNDSVGRTCFAPAPGDSAWYTFTYS
                     YLDDCEGSDRAPVSPQWLVDSVRVLVVPNRPPQLVCPDPQEFSTCALDTFCFEVEALD
                     PENGPLEFAVISGNAYLDETGKVCFAGEESDAFEIALTVTDECGLTDTCTVPVTVSGN
                     RPPYVTMAADFAVALCAPETVCFAAAVDDPDFDIASVTASFGIWDEATDRLCFTADTA
                     GVYVLVVTAADSCGIVAADPTVVTVSRPAPPTVS"
BASE COUNT          554 a         1032 c         1070 g          646 t
ORIGIN      
        1 ccgcaccgca agccggcctg caggaatgct cccgcacctg tccggttcgg ctgatctccg
       61 ccgaatcgcg gtatgcttgc tcctacgcct gcggcagtca tgtccgtgtc ctttccgcca
      121 cccgcgggat cagtcaacat cccacgggtt ctcgaccatc ggcggttcgc cgaccggcaa
      181 atggtctttc aacccaatga gttggcacat atttgccaag atcaagtcct ttctttttct
      241 acacgcggcc ggccattttc ttcccgccgg gtcaatcccg gcgccgaccg tcacgcggct
      301 tacgtgcgct caggcacacg taccctagct tggatacgca gcgagacgca attggtttcc
      361 tttgcagagg ggaaatcggc gctggggtta ttccgcgtcg ggccccgcaa tcgactgatt
      421 ggtaacgcct tacgtgattc tcctgcccct ccgcggggcg aggtcgggca agtggcgtgg
      481 acggctcttg gcctccgccg gggcggacct ggtgggcggg ggatgtcacg ctgcggcaaa
      541 gcggtctgcc cggcggcgcc gggctcgttt accgctggtt cacaagttgc gccgtcaggc
      601 gctggatgtg ccaggtatgg acccgctcgt gccagagagc ccggcgggcg actttgcggg
      661 ccgaccaaaa ctccccgtgc ccctcgctca ccgtcgcatc attggcgaag cggggaaggt
      721 gcatgagcag gtggcggcgg accgattcca gcatcgcgaa gggatcggtt ccgagagtct
      781 ctttctgcgg cgccagaccg agcttgtcca gataccagcg ctcggccgtg gccgcgtgcg
      841 tgaggatccc ggcgatcgtg ccgaagcgct cccccgcgat gggctggtcg agggccgcgg
      901 ccggtacccg gcgcacggcg gaaatcaggt ccgaccgcgt gcagctcaac agccaccgca
      961 cgtcgtcgat ctcacccgcc aggaggggac ggcggtccca ggcgaagaag gcattcaccc
     1021 agtacccgtc gagcgtctcg tagttcctga attcctcgac cacctcgata cccatctgct
     1081 cgatcgcctc gctgctgacg cgctcatacc cgtcccgcca gttgaagtaa tcggcgatag
     1141 cccgcggggc gcaccgcacg acttcgtcac gcgccgaggc ccgggcaaaa catccgggca
     1201 ggtcaaccac ccaggcaatc catttgccgg gttcggtctg ctccagaccc agagcgaatt
     1261 tcatgattcc gctgcaccgc agttttcagg tgaacctcag accaaggtaa tgcggcagtc
     1321 gacggatgta aaggacagac cgccgtgtcc tccgggtttg cggccggagg cggcgcaccg
     1381 actgctccga ggcggggcgg gttgaaatct gactgtcgac cgatgatcgg cgcggggttc
     1441 agatgcgccg gtaggcaagg aaaagcacaa tctgtcacag cggcttcaca attagggcgg
     1501 ggcggttgca ggagaggggc cggggccggc gtaagttcat tgagttcagg ccgttaatct
     1561 gttggcccga ttcttgcgtc actgttcctg cggagtgaaa tgaccttgac ttttagggac
     1621 cggaaagtat atttgtatgg ccaaagagac ccgcgcaatt actgcactgg cgcatcgagt
     1681 ttatacgtcc acgattggtg tggggttacc ggcgctaaat gttgcgttga caggtttcct
     1741 atgccccgtc ggcgcatgga aatatggcac tactggatcg atttgacact gattccaggc
     1801 agctgtccgg tggggactcg agcagccggg cagttcgacc gagaggaaag atgaaacact
     1861 cagggattcc gcgaattcca atgctgtttg tcctcaccct tatctgcagc gccggcgcct
     1921 gggctcaggc ggaggcggca acgatcgcca ccgattcggt ggccctctgt ttccaggagg
     1981 cgctcttcga tacgattgcg gtggacctgc cgaacccggg caatccgggc gtcgccatgg
     2041 cgctcgtcag cgggccgggg gcgttgacat acagcgtgtc cgacaagctc tacggttact
     2101 atgaatacgc gccgtccggc gacaccgcct tcgagatcgt ctaccgggtg tggtatgccc
     2161 cgacggactc ggcggatttc cggcaccgct acattgtgtg ggtcgatgag ccgcccactc
     2221 tgggcgacca gtacaacgcg ttcaaggtct gctggcccgg tccgcactac ttgaaagtgt
     2281 acgcgtggga tcccgagtcg aggccgctcg cctgggaaat tgtgtcggga ccggggacaa
     2341 tcgacagtca ggggcttatc acctacacgc ccgacacggc gggagtgtac gtttttgagg
     2401 tcgcggcgag cgacgactgc aatagggcgg cggccacggt ctatgacacc gtccgcttca
     2461 acggcgcgcc gcgaattgtc ctcgccgaca gcattttcag cctctgcgcc gagggggaga
     2521 tctgtttcga cgtggtgttt tcggatccgg atggagacgc cgtctggttc aaccaggtgg
     2581 gcgggcccgg gtcaaccacg gtcgtgaatg attcggtcgg gcgcacctgc ttcgcgccgg
     2641 cgcccggcga cagcgcctgg tacacgttta cgtattccta tctggacgat tgcgagggca
     2701 gcgacagggc gccggtgagc ccccagtggc tggtggattc ggtgcgcgtc ctggtcgtgc
     2761 cgaaccggcc gccgcagctg gtctgtcccg acccccagga gttttccacc tgcgctctcg
     2821 ataccttctg ctttgaggtc gaagcgctcg atcccgaaaa cgggccgctg gagttcgccg
     2881 tcatctcggg aaacgcgtac ctcgacgaga ccgggaaagt ctgctttgcg ggcgaggaat
     2941 cggacgcgtt cgagatcgcg ctcacagtga ccgacgagtg cggtttgacc gatacctgca
     3001 cagtcccggt gaccgtgtcc ggcaaccgtc cgccctacgt gaccatggcg gcggattttg
     3061 cggtggcgct gtgcgccccc gagaccgtct gtttcgcggc ggcggtcgac gatccggatt
     3121 tcgatattgc ctcggtgacc gcctctttcg gcatctggga tgaggcgacc gaccggctgt
     3181 gcttcaccgc cgataccgcg ggcgtgtacg tgctggtggt gacggccgcc gattcctgcg
     3241 gcatcgtcgc cgccgacccg acggtcgtga cggtgtcgcg gccggctccg ccgaccgtga
     3301 gc
//
LOCUS       DAOPOE010000177         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_32686_length_3207_cov_5.247779, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000177 DAOPOE010000000
VERSION     DAOPOE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32686_length_3207_cov_5.247779"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKM94_13880"
     CDS             complement(<1..203)
                     /locus_tag="PKM94_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67767.1"
                     /translation="MRKIHVTLFIPAAVLLWAVGGRAAETVKVGDPAPAFTLPFATRD
                     SVDLKGLSSAELAGKRYVLAFYPA"
     gene            complement(312..623)
                     /locus_tag="PKM94_13885"
     CDS             complement(312..623)
                     /locus_tag="PKM94_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67768.1"
                     /translation="MADSGLLCWNCGRATGVEGHPTRNDSCPHCLADLRACRGCRHFD
                     PYARAQCRETIEAPVRDKEKSNFCDFFQVRDAIKRPGGIRASTDSKEERKKRFDDLFN
                     D"
     gene            complement(702..1712)
                     /locus_tag="PKM94_13890"
     CDS             complement(702..1712)
                     /locus_tag="PKM94_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOD67769.1"
                     /translation="MVHKVTLIPGDGIGPEITEAAVRVIEAAGVEIAWDRQEAGIPAV
                     QTHGTSVPDQLLDSIHANKVALKGPITTLVGKGFRSANVTLRQRLDLYVNLRPVRTIG
                     GVPSRYSKVNLVIVRENTEDLYSGIENMITPGVTQAIKVVTERASERVARWAFNYARK
                     MGRKKITFVHKANIMKLSDGLFLKVFEDVAKDYPDIEAEDRIVDALCMRLVMDPTQFD
                     MLLMGNLFGDIVSDLAAGLVGGLGVVPGANIGDEYAVFEAVHGSAPDIAGKNLANPTA
                     LIFSALLMLRHLGEDAAADRIWKSMVVTLAEGHKLTRDLGGTCSTTEFTDELVREIKS
                     RT"
     gene            1882..2934
                     /gene="selD"
                     /locus_tag="PKM94_13895"
     CDS             1882..2934
                     /gene="selD"
                     /locus_tag="PKM94_13895"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1942..1944,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOD67770.1"
                     /translation="MEDKRKQTTVRLTAEVTCAGUASKLAPQLLADALRNLAVSTHPD
                     LLVGFNKADDAGVFRLSETVALVQTVDFFPPIVDDPFDFGRIAAANALSDIYAMGGRP
                     LTALNIVGFPAAMDPWILGEILRGGAQKIEEAGAVIVGGHSVNDKEVKYGVAVTGLID
                     PRKVVTNAGARPGDRIYLTKPLGTGLITTGLKRGAVDPALVALVTAQMAELNREAAEA
                     MVAVEARAATDITGFGLLGHAYEMAAASEVTIRLAADRLPLLPEAARLAGAGMIPGGA
                     GANRAFLAGKVSVAPTVDKNLEHVMYDPQTSGGLFVAVPPDRALQFERELQKRSVPGR
                     CIGEVEPRGAVALLVA"
BASE COUNT          496 a         1153 c          991 g          567 t
ORIGIN      
        1 gccggataga acgccagcac ataccgtttc ccggccagtt ccgccgagct gagccctttc
       61 aggtcaacgg aatctctggt cgcaaacggg agcgtgaacg ccggcgccgg gtcgccgact
      121 ttgactgtct cagcggcacg gccgcccacc gcccagagaa gcaccgccgc cgggataaac
      181 agagtgacat ggattttgcg catcgccgtt tctctcgtga ttggtttttc ggctcagaac
      241 ggagcggccg gcccgggggt tccccggtcg cgccggaacg ggagtgcggc gtccggaccc
      301 gcgtccggag gctagtcgtt gaagaggtcg tcgaaccgct tcttccgctc ttccttgctg
      361 tccgtcgagg cccgaatccc ccccggccgt ttgatcgcat cgcgaacctg aaagaagtcg
      421 cagaagttgc ttttttcctt gtcccgcacc ggagcctcga tcgtctcgcg gcactgggcg
      481 cgggcgtagg ggtcgaaatg ccggcacccg cggcatgcgc gcaggtcggc caggcagtga
      541 gggcaactgt cgttccgcgt cggatgtccc tccacgcccg ttgcccgtcc gcagttccag
      601 cacaacagac cgctgtcagc catgatcaac ctcctccggc agacgccgcc gccgcggccg
      661 gttcccgcgc gcggactttc atgaatccgc gccgcggcgg ctcacgtgcg actcttgatc
      721 tctctcacca gctcgtcggt aaactcggtc gtcgagcacg tcccgccgag gtcccgcgtg
      781 agcttgtgcc cctccgcgag cgtcaccacc atcgatttcc atatccggtc ggccgccgca
      841 tcctcgccga gatgccgcag catgagcagg gcggagaaga tgagggcggt cgggttcgcg
      901 aggttcttgc ccgcgatatc cggagccgag ccgtgcaccg cctcgaacac cgcgtactcg
      961 tcgccgatgt tcgcccccgg caccaccccg agcccgccga ccagcccggc cgcgagatcg
     1021 gagacgatat cgccgaacag gttccccatg agcagcatgt cgaactgcgt cggatccatg
     1081 accagccgca tgcacagggc atcgacaatg cggtcctccg cctcaatgtc gggatagtcc
     1141 ttggcgacgt cctcgaacac cttgaggaaa agaccgtcgg agagtttcat gatgttcgcc
     1201 ttgtgcacga acgtgatctt cttccggccc attttgcggg cgtagttgaa ggcccaccgg
     1261 gccacccgct ccgacgcccg ctcggtcacc accttgatcg cctgcgtcac ccccggcgtg
     1321 atcatgtttt cgatccccga gtacaagtcc tcggtgttct cccgcacgat cacgaggttg
     1381 accttgctgt accggctcgg cacgccgccg atggtccgca ccgggcgcag gttcacgtac
     1441 aaatcaagcc gctggcggag cgtcacgttc gccgaccgga accccttccc caccagcgtc
     1501 gtgatcggcc ccttgagcgc cactttgttg gcgtggatcg agtcgagcag ttggtcgggc
     1561 accgacgtgc cgtgggtctg gaccgccgga atgccggcct cctggcggtc ccaggcgatt
     1621 tctacccccg ccgcctcgat gacccggacg gccgcctcgg tgatctccgg gccgatccca
     1681 tcgccgggaa tgagcgtcac tttgtgcacc atgctgatgt accggcatcc tttctgtaat
     1741 gggcgccgca gtatatcgca tttccgcaga cctgtcaact tcccctccgc gcgcgatccc
     1801 cggttgactt tgcccccgcc tttggcgtac cttctccgat gactccccgg ggccgtgggg
     1861 cagccgggaa gcgcgacccg tatggaagac aagcgcaaac agaccaccgt ccgcctcacc
     1921 gccgaggtga cctgtgcggg gtgagcgtcc aagctcgcgc cacagttgtt ggcggacgca
     1981 ttgagaaacc tcgcggtgtc gacccacccg gacctgctgg tcggcttcaa caaggccgat
     2041 gatgccgggg tatttcgtct gtcggaaacg gtcgccctgg tccagacagt cgactttttc
     2101 ccgccgatcg tcgacgaccc cttcgatttc ggccgcatcg ccgccgccaa cgccctctcg
     2161 gatatctacg ccatgggagg ccgtccgctg accgccctca acatcgtcgg cttcccggcc
     2221 gccatggacc cgtggatcct cggcgagatc ctccgcgggg gcgcgcagaa gatcgaggag
     2281 gcgggcgcgg tgatcgtcgg cggccactcg gtcaacgaca aggaggtcaa gtacggcgtg
     2341 gccgtcaccg gcctcatcga tccgcggaag gtggtcacca atgccggcgc ccggcccggc
     2401 gaccggatct atctcaccaa gcccctcggc accggcctga tcacgaccgg tctcaagcgc
     2461 ggcgcggtcg accccgccct ggtcgcgctg gtcaccgcgc agatggcgga gctcaaccgc
     2521 gaggcggccg aggcaatggt cgctgtcgag gcccgggccg cgaccgacat caccggcttc
     2581 ggccttctcg gccacgccta cgaaatggcc gccgcctccg aggtcaccat ccgcctggcc
     2641 gccgaccgcc tgccgctcct tccggaggcg gcccggttgg cgggagcggg gatgatcccc
     2701 gggggcgcgg gggccaaccg cgctttcctc gccggcaaag tctcggtcgc cccgaccgtc
     2761 gacaaaaacc tcgaacacgt gatgtacgat ccgcaaactt ccggcgggct gttcgtcgcc
     2821 gtcccgccgg accgagcact gcagtttgaa agagaactcc agaaacgcag cgtccccggg
     2881 cgatgcatcg gggaagtcga accccgcggg gccgtcgcgc tcctcgtcgc ctgagccggc
     2941 gccggccgcc ggcgcggctc tgggccgcgg ccgctggctg ctcctgctgg ccgccgcgat
     3001 cgtcgccggg ggcttcatct ggtggcagtc ggatctcgtc aaagatccgc ccatgtattt
     3061 ctccggcatc gagcagtcgc tcggtaccga tcccgcgcag tatgtccacc acgcccgcaa
     3121 ccggatcctt ttcggcgact gggacccgtt tgactacccc cgctgggcgg tctaccggca
     3181 ctccctcgtg tcgctcgtgt cgtgggt
//
LOCUS       DAOPOE010000178         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_34653_length_3070_cov_4.955556, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000178 DAOPOE010000000
VERSION     DAOPOE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34653_length_3070_cov_4.955556"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKM94_13900"
     CDS             <1..949
                     /locus_tag="PKM94_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67771.1"
                     /translation="LLFFATAAAAVGSNAAAVARRLPGEWLKAGAHLTHFGFGLMLLG
                     VLASSAFSTGERVTLPQGQPRPAYHLQVSYEGMQNDIKWPHNELILKVVDDGRVDEGR
                     PELYFSERMNGYMKRPYIRRGLLYDLYFSPQDIKQQEGQDGLLIGKAEKASAGGYEFS
                     FLGFDMGDHSGGGEMTVTALIAAARDGRVDTLRPAVTMATADDGSTALQDLPAAFGSG
                     DSTFTMSITRIHADAGAVAVAIPGLLAPGAGDELLLDVSKKPLINLVWIGTTLILLGT
                     LAAWLRRRRQTALAAESAAKGPGAETGDRPAVPFTRKQN"
     gene            complement(1046..1468)
                     /locus_tag="PKM94_13905"
     CDS             complement(1046..1468)
                     /locus_tag="PKM94_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67772.1"
                     /translation="MSGWSFEYDIEVDEANRIVYEKIFGVWRIKTAESYRADFEEEVK
                     PIIDRPWAKLIDLSNWKTSYPEVIDIVGKHLAWCRRHNMQWSVNVINNTITYNQLQRM
                     FDKGATKEISRTFRNYAEAVQFLQEQGYTVRPTPPSRT"
     gene            complement(1680..>3070)
                     /locus_tag="PKM94_13910"
     CDS             complement(1680..>3070)
                     /locus_tag="PKM94_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD67773.1"
                     /translation="SGVERLIAGARADLLSGAHRLALAARYFRDSLGAPGPVPDPSFI
                     PVYGDPESSSLTDRQEDEHYSVDLQYSLVDERAGETQIGLFWEKKKLTYRSLYNYLLF
                     SATTDSVDVRTETVYDKRSAGANLRHRHNFGPLAAAGGVDWLSGSLQYANAERSEAAA
                     IDGPGAPAVSSYDTFASYDAGQDQVDLWGNAQMGRAGRVHVDLGGRLQLVAGRRPQPS
                     YNLGLVAEPGDALRLKLAYAYAFRLPSLAEQFADDLYTAGNADLSPETARTLAATMAL
                     EPAALPVRLSLTWFRQEIDSLIQYVWDPASYRSVPQNFLRFISQGLDATCTIALRRGL
                     SWRGSAVYQAAEQTAADGRTMREAPYVPEWKWRVGMTWRSGRFDAAARLDGTSERVLY
                     LYGGAPKTIDPVVEAAAELGVRLSAMLRMHISGEDLTDRRRPDQFGFTAGDGDYPGLG
                     RRFFVELALAMR"
BASE COUNT          506 a          999 c         1033 g          532 t
ORIGIN      
        1 cctgctgttc ttcgccaccg cggcggccgc ggtcgggtcg aacgccgccg ccgtggcgcg
       61 ccggctgccc ggcgagtggc tgaaagccgg cgcccacctc acccacttcg gtttcggcct
      121 catgctcctg ggcgtgctcg cctcctcggc cttctcgacc ggcgagcggg tcacgctccc
      181 gcagggacag ccgaggccgg cctaccatct ccaggtcagc tacgaaggga tgcagaacga
      241 catcaagtgg ccgcacaacg agctgattct gaaggtggtg gacgacggcc gggtcgacga
      301 ggggcggccg gagctctact tctccgagcg catgaacggg tacatgaaac ggccgtacat
      361 ccggcgcgga ctcctgtacg acctgtactt ctcgccgcag gacatcaagc agcaggaggg
      421 gcaggacggg ctgttgatcg gcaaagcgga gaaagcctcg gccggcggat acgaattctc
      481 gtttctcggt tttgacatgg gcgaccacag cgggggcggt gagatgaccg tgaccgcgct
      541 gatcgccgcc gcccgcgatg gccgtgtcga cacgctccgc ccggccgtga ccatggccac
      601 ggccgacgac ggcagcaccg cgctgcagga tctcccggct gcgttcggct ccggggactc
      661 gactttcacg atgtcgatca cgcgcatcca cgccgacgcg ggggccgtcg cggtcgccat
      721 ccccgggctc ctggcgccgg gagcgggcga tgaactgctg ctcgatgttt cgaaaaagcc
      781 gctgatcaat ctggtctgga tcgggacgac tctcattctg ctgggaacgc tggcagcgtg
      841 gctgcggcgc cgccgtcaga ccgcgctcgc tgccgagagc gcggcgaagg gtcccggcgc
      901 cgagaccgga gaccggccgg ctgttccatt cacgcgaaaa cagaactgat cccgctgggg
      961 gagtgacgaa agtcaggcgc cgctcgctcc ggctgcttct cggtgttcgc cgggctgtcc
     1021 ctctcggcgc gcctctccgg cggatctacg tgcggctcgg cggggtgggg cggacggtgt
     1081 acccctgctc ttggaggaac tgcacggcct cggcgtagtt gcggaaggtg cgggagattt
     1141 ccttggtcgc ccccttatcg aacatgcgct ggagctggtt gtaggtgatg gtgttattga
     1201 tgacgttcac cgaccactgc atgttgtggc gccggcacca ggcgaggtgt ttgccgacaa
     1261 tgtcgatcac ctcggggtag ctggttttcc agttggagag gtcgatcagt ttggcccagg
     1321 ggcggtcaat aatcggtttg acttcttcct caaaatccgc ccggtaggat tcggcggtct
     1381 tgatgcgcca gaccccgaag attttttcgt agacgatgcg attggcctcg tcgacctcga
     1441 tatcgtattc gaaactccac ccgctcatgg taaaccggat cccttccttt ttggagccac
     1501 actctctgcg atttcggata ctgccgtgcc gcgcccggag ccgggacggg tgatactcgc
     1561 tcgcatctta aacgccgcgc cgccggatag ggttccgccc gacgtttggt cgcctcctca
     1621 agggcccctg aaagttctca acaaatcagt gctgctgaga gaaccgagtt agtctcagtt
     1681 tacctcattg ccagcgccag ttcaacaaaa aaacgccgtc cgagtccggg gtagtcgccg
     1741 tcccccgcgg tgaacccaaa ctggtcgggg cggcgccggt cggtcagatc ctctccggag
     1801 atatgcatcc gcagcatcgc actcaggcga accccgagtt ccgcggccgc ctcaaccacc
     1861 ggatcaatcg tcttcggggc gccgccatag aggtacagca cccgttccga ggtgccgtcg
     1921 aggcgcgccg ccgcgtcgaa acgtccgctg cgccaggtca tcccaacccg ccatttccat
     1981 tcggggacat acggcgcctc gcgcatggtc cggccgtcgg cagccgtctg ctcggccgcc
     2041 tggtacacgg ccgagccgcg ccacgacaga ccgcgtcgga gcgcgatcgt gcaggtcgca
     2101 tcgagtccct gcgatatgaa ccggaggaaa ttctgcggaa ccgagcggta gctcgccgga
     2161 tcccacacgt actggatgag ggagtcgatc tcctggcgaa accaggtcag agagaggcga
     2221 acggggagag cggcgggctc gagcgccatc gtcgcggcca gcgtgcgggc cgtctccggg
     2281 ctcagatcgg cattcccggc cgtgtagagg tcatcggcga actgctcggc cagcgagggg
     2341 aggcggaaag catacgcgta agcgagcttg agccgcagcg cgtcacccgg ctcggccacc
     2401 agcccgaggt tgtacgaggg ctgcggccgc cgccccgcta ccaattggag ccggccgccg
     2461 aggtcgacat ggacgcgccc ggcccggccc atctgcgcgt tcccccacag atccacctgg
     2521 tcctggccgg cgtcgtacga ggcgaacgtg tcgtagctgg agaccgcggg agcccccggg
     2581 ccgtcgatag ccgcggcctc gctgcgctcg gcgttggcgt actgcagcga accgctcagc
     2641 cagtcgaccc ctccggccgc cgcgagcggg ccgaaattgt gccggtgccg gagattcgcc
     2701 ccggccgagc gcttgtcgta gacggtttcg gtccgcacgt ccaccgaatc ggtggtcgca
     2761 gagaacaaca ggtagttgta gagtgaccgg taggtgagct tctttttctc ccaaaacagc
     2821 ccgatctgcg tttcgccggc ccgctcgtcg acgaggctgt actgcagatc caccgaatag
     2881 tgctcgtcct cctgccggtc ggtgaggctg gaggattcgg gatcgccata gacggggatg
     2941 aatgacgggt ccgggacggg gccgggggcg ccgagggagt cgcggaagta gcgtgccgcg
     3001 agggccaggc ggtgcgctcc ggagagtaag tcggcgcgcg caccggcgat gagccgttcg
     3061 acgccggagt
//
LOCUS       DAOPOE010000179         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_35115_length_3039_cov_4.891421, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000179 DAOPOE010000000
VERSION     DAOPOE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35115_length_3039_cov_4.891421"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..893)
                     /locus_tag="PKM94_13915"
     CDS             complement(201..893)
                     /locus_tag="PKM94_13915"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOD67774.1"
                     /translation="MMKVLGGIVGIGLLTAATAFGQARLVAPLDKFDFGILPPNSVLV
                     HHFWLRSAGADTVTITDVKTGCSCAASELEKNVLAPGDSVLLGIRWDIGRSHGSIFRS
                     PLVFFNNSEQTLRLSLEGQVWDDPETARPVTVKPFRFELSRAAIKDIDGLPFTLTNHS
                     GEELSIELLSLAPEEIELVLPEKIPANGSASGSVRVKPEFRNTEFSTSITLLVRDERN
                     THVTIPVRRKFY"
     gene            complement(1058..2425)
                     /locus_tag="PKM94_13920"
     CDS             complement(1058..2425)
                     /locus_tag="PKM94_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOD67775.1"
                     /translation="MDDFLSRLAGLVPPEQFSTHPDHLRVAAKDESTTTPVTPRAVVW
                     AVTIDEIAAVVRLCRAHRVPITTRGGGSALEGSAVPVEGGIVLDLSRMTAILGYWPED
                     LQVEVQPGLIYDDLNRRLKDDGLFFPPSPGGSGDVATIGGMVSTNASGIYSVKYGGTR
                     DYVLRLRVVTGTGEILTLGDRAVKRSSGYNLVDLISGSEGTLAIIGAVTIRLAGLPEG
                     RRQEAYAFGNEVAASRAVSEMRRYGLDLAAIEFLDGRLLEALNRLKEYGLTEAPSLFL
                     EYHGPEAVLASNAELAAGICEEMGGRRITLAEGQNPWEIRHWATDAVKHRRPGYSILR
                     NDIAFPISRLAELVEFCYRTADAAGLAIHTFGHVGMGLLHALLLARREDAAEWSAARQ
                     TNEMIIRKALEVDGTISGEHGIGLGHKRLFQEHHGDTVDLMRRIKAQFDPANILNPGK
                     IFDPS"
     gene            complement(2438..>3039)
                     /locus_tag="PKM94_13925"
     CDS             complement(2438..>3039)
                     /locus_tag="PKM94_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD67776.1"
                     /translation="FYDEIFGGPLPDYAILMDNPAKGYDTLYRIESSRNMADRADLTM
                     LNLPVARLKEYCLRALLDSQAVWFACDVGVDNFGDSGILRNGIYDFEGTLGVSFAIAK
                     ADRITLRDSYPNHAMTLVGVDTAADGSPVKWLVANSWGDKGGNDGYWIMYNDWFDTNV
                     YLVAVEKRLLAEADRKKLDQAPVVLPPWDPFYQAAKRLK"
BASE COUNT          551 a         1036 c          888 g          564 t
ORIGIN      
        1 acccgaaatc gaaatccgat tcgggcatcg tgagccgggg agctccccag gccgaccccg
       61 ccacaagcgc cgccgccgcc agcccgaggc atgtggtttt gagtcttgtc atgttcgtcg
      121 tctgacccct ggtaaaaacg gtttgttctg taactctata atatacgcac taaatcccct
      181 tttgtcgacc ccaaacctgc ctagtagaac ttccgccgca cgggaatcgt gacgtgcgtg
      241 tttcgctcgt cccggaccag cagcgtgatc gacgtgctga actccgtgtt ccggaactcc
      301 ggtttcactc gcaccgaacc gctcgccgag ccgttggccg ggatcttctc cggcagcacc
      361 aactcgattt cctccggcgc tagcgagagc agctcgatcg acaactcctc gccggaatga
      421 ttcgtcaggg tgaacggcag gccgtcgata tcctttatcg ccgcccgcga cagctcgaac
      481 cggaacggct tgaccgtcac cgggcgggcg gtctccgggt cgtcccacac ctgcccctcc
      541 agcgacaacc gcagagtctg ctcggaattg ttgaagaaga ccagggggga ccggaagatg
      601 gagccgtgcg agcgaccgat atcccaccga atccccagca gcacgctgtc gcccggcgcc
      661 agcacgttct tctccagttc gctcgccgcg caggaacacc ccgtcttgac atcggtgatc
      721 gtcaccgtgt cggcgcccgc cgagcgcaac cagaagtggt gcacgaggac tgaattcggg
      781 gggagaatgc cgaagtcgaa cttgtccaaa ggggccacca ggcgcgcctg gccgaaagcc
      841 gttgccgccg taagcaggcc gatccccacg atgccgccga gaactttcat catacccctt
      901 tataacgtat ttctacagtt cggttccgcc aaatcgccat gcaagataga cggcctgacc
      961 gggcccgtca agaactaccc ggttgcgccg caacctgtta ataatcaagt gcatataccg
     1021 acggtgcggt ggcgccgggc gccccgcttt ccccggctca tgacggatcg aagatcttgc
     1081 cgggattgag aatattcgcc gggtcaaact gcgcctttat ccgccgcatc aggtcgactg
     1141 tgtcgccgtg gtgttcctga aagagtctct tgtggccgag accgatgccg tgttcgcccg
     1201 agatcgtccc gtccacttcg agcgcctttc ggatgatcat ctcgttagtc tgccgggccg
     1261 ccgaccactc ggccgcatcc tcccgtcgcg ccagcaagag cgcgtgcaga agccccatgc
     1321 cgacatgccc gaatgtgtga atggcaaggc ccgccgcatc cgctgtccgg tagcagaact
     1381 ccaccaactc cgccaggcgg gaaatcggga aagcgatgtc gttgcggaga atggagtatc
     1441 cggggcgacg atgcttcacg gcgtcggttg cccagtgccg gatctcccac ggattctgcc
     1501 cttccgccag cgtgatccgc cggcctccca tctcctcgca gatcccggct gcaagttcgg
     1561 cgttggacgc aagcacggct tccgggccgt ggtactccag gaacagcgaa ggggcctcgg
     1621 tgaggccgta ctccttcagg cggttcaggg cctccagcag ccggccgtcg aggaattcaa
     1681 tggccgccag atccagtccg tagcgccgca tctccgacac cgcccgcgag gccgctactt
     1741 cgttcccgaa ggcgtaggct tcctgccgcc gtccctccgg cagcccggcc agccggatcg
     1801 tgaccgcgcc gatgatcgcc agcgttccct ccgacccgct gatcagatcg accaggttgt
     1861 agccggagga tctcttgacg gcccggtcgc cgagcgtgag aatttcccca gtcccggtga
     1921 ccacccggag gcgcaggaca taatcacgcg tgccgccgta tttcactgaa tagatgccgc
     1981 tggcgttggt cgagaccatg ccgccgatgg tcgctacatc acccgagccg cccggtgagg
     2041 gagggaagaa caacccgtcg tctttcaacc tgcggttcag gtcatcatat ataagtcctg
     2101 gttgtacttc gacttgcagg tcttccggcc agtatccgag aatcgccgtc atccggctga
     2161 gatcgagcac aattcctccc tccaccggta cagccgatcc ctccagcgca ctccctcccc
     2221 cgcgggtcgt aatcggaacc cggtgagcgc gacagaggcg aaccacggcc gcgatctcgt
     2281 cgattgtcac agcccagacc accgcgcgcg gcgtcaccgg cgtcgtcgtc gattcatcct
     2341 tcgccgccac ccgcagatgg tcggggtggg tgctgaactg ctccggcgga accagccccg
     2401 ccagtcggct cagaaaatca tccatggtcc gcgccgccta cttcaggcgc ttggcggcct
     2461 ggtaaaacgg atcccagggc ggcagcacca ccggcgcctg gtcgagcttt ttccggtcgg
     2521 cctccgccag cagccgcttc tccaccgcca ccaggtacac gttggtgtca aaccagtcgt
     2581 tgtacatgat ccagtaaccg tcgttgcctc ctttgtcccc ccagctgttg gccacgagcc
     2641 acttgacggg cgacccatcc gcggcggtgt caaccccgac cagcgtcatg gcgtggttcg
     2701 ggtagctgtc gcgcagggtg atgcggtcgg ccttggcgat agcgaacgac accccgagcg
     2761 tcccctcgaa atcatagatg ccgttgcgga gaatgccgga atcgccgaaa ttgtccacgc
     2821 cgacgtcgca cgcgaaccag accgcctgcg agtcgagcag cgctctcagg caatactcct
     2881 tgagccgcgc gaccggcagg ttgagcatgg tcaggtccgc ccggtcggcc atgttgcggc
     2941 tgctctcgat ccggtagagc gtgtcgtacc ccttggccgg gttgtccatg aggatggcgt
     3001 agtcggggag cggtcccccg aagatctcgt cgtagaacg
//
LOCUS       DAOPOE010000180         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_35183_length_3035_cov_4.239262, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000180 DAOPOE010000000
VERSION     DAOPOE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35183_length_3035_cov_4.239262"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKM94_13930"
     CDS             <1..387
                     /locus_tag="PKM94_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67777.1"
                     /translation="PVVDRYRSEIEASALVPIPLHPRREHYRGYNQARVFAEALGKRL
                     GLPVETELLARVRHRRPQARIDRRRREVNVHGVFETADDDGPRLGGLILVDDVVTTGA
                     TIREARRVLEASGHGVAGAAAMAHGV"
     gene            391..1323
                     /gene="xerD"
                     /locus_tag="PKM94_13935"
     CDS             391..1323
                     /gene="xerD"
                     /locus_tag="PKM94_13935"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOD67778.1"
                     /translation="MGTVAAHIEDFLGYLKLERGMAANSVAAYRRDLCEFARLAAPGR
                     KGEAAGQVAVERIDSRAAAAYLKHLTSKGRKPATMARKISSLKQFFNYLVDSAVIATN
                     PVAGLAAPRIARYHPHYLSPREIEQMIDSIDTTQRHGWRDRMIIELLYGSGLRISELI
                     DLELGDIEFEAGFIRVTGKGNKQRLVPLGGYARRAIDEYLAEAPDRKVIDGANVLLRN
                     DLGGKISRVGVWKIIKKAVLKAGITKPVSPHTLRHSFATHLLEGGADLRVVQEMLGHA
                     DISTTQIYTTIDRDYIIAEHRKYHPRELARPKDQ"
     gene            1268..2266
                     /locus_tag="PKM94_13940"
     CDS             1268..2266
                     /locus_tag="PKM94_13940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD67779.1"
                     /translation="MRSIESTILGSWPGPKTSEAPKRPTYHFAVKQVGVDLDFHLRQF
                     FAGQEVSFHYFGSVETLLTICHRFPVDAIVIASRYDLTVEVEMIRAIKDNVFLSIIPV
                     IVYHPDPPTDVMVAAYEQGAEDFIYGEWRDKLVKVRIRTAIERSRRDLSINPSTRLPG
                     PAIIEREIQRMLEMQAEFAVGYVDLDNFKAYNDYYGYYRGDKVIQLTARVVKDAVCDL
                     CDGGFVGHIAGDDFIFMVPVDQVERVCQAVIRTFDTLIRFCYEEEDRERGYITTKSRR
                     GEIENFPLLTVSIAVLLNRNGTFAHIGEMAKMLADLKKATKQKAGSNYMVERRGKY"
     gene            2722..>3035
                     /locus_tag="PKM94_13945"
     CDS             2722..>3035
                     /locus_tag="PKM94_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67780.1"
                     /translation="MWKRLIVLSAGLLIGVPWSGCGSGPEESADVGPEPPSRVVGMLQ
                     GGLTWPASISVWFEHPDPASIDSFLIYRKIGDGSFAPVKVKTLQPAAGTGNPKGGEPG
                     DVP"
BASE COUNT          578 a          903 c         1034 g          520 t
ORIGIN      
        1 ccggtggtcg accggtaccg gagcgagatc gaggccagcg ccctcgtgcc tatacctctg
       61 cacccgcggc gcgagcacta ccgggggtac aaccaggcgc gcgtctttgc ggaggcgctc
      121 ggcaagcggc tggggctgcc ggtcgaaacg gagctgctgg cgcgcgtccg acaccgccgg
      181 ccccaggcac ggatcgaccg ccgacggagg gaagtgaacg tgcacggggt tttcgaaact
      241 gccgatgacg acgggccgcg cctcggcggc ctcatcctgg ttgatgatgt cgtcacgacc
      301 ggggcgacca ttcgagaggc gcgccgcgtg cttgaagcgt ccgggcatgg ggtggccggg
      361 gcggcggcga tggcgcacgg agtctagatc atgggcacag tcgcagcaca catcgaagac
      421 ttcctcggct acctcaagct ggagcgcggg atggcagcca attcggtggc cgcgtaccgg
      481 agagatctgt gcgagtttgc ccggctggcg gcgcctggca ggaaggggga ggctgccggt
      541 caggttgcgg tcgaacgtat cgacagccgg gccgcggcgg cgtacctgaa gcatctcacg
      601 tccaaggggc ggaagccggc caccatggcg cgcaagattt cctccttaaa gcagttcttt
      661 aactatctcg tcgattccgc tgtcatcgcg actaatccgg tggcgggtct cgccgcgccc
      721 agaattgcga ggtaccatcc gcactatctg tctccccggg agatcgagca gatgatcgac
      781 tcgatcgaca cgacccagcg ccacggctgg cgcgaccgga tgatcatcga actcctgtac
      841 ggcagcgggc tgcggatctc agagttgatc gacctggagc tgggagatat cgagttcgag
      901 gcggggttca tccgggtgac cggcaaggga aacaagcaac ggttggtgcc gctcggggga
      961 tatgcccgga gggcgattga cgaatacctg gccgaggcgc ccgacaggaa ggtgatcgac
     1021 ggggccaacg tgttgttgag aaacgactta ggagggaaaa tctctcgcgt gggggtatgg
     1081 aaaatcatca agaaagcggt gctgaaagcc gggatcacta aaccggtgtc gccccacacg
     1141 ctccggcatt cctttgccac ccatttgctt gaaggggggg cggatttgcg cgtcgtacaa
     1201 gaaatgctcg gtcatgccga tatatctaca acacagatct atacgaccat tgaccgggac
     1261 tatatcattg cggagcatcg aaagtaccat cctcgggagc tggcccggcc caaagaccag
     1321 tgaggctccg aaacggccca cctaccactt tgcggtcaag caggttgggg tcgacctcga
     1381 tttccacctt cggcagtttt tcgccggtca ggaggtgtcc ttccattact tcggctcggt
     1441 cgaaacgctg ctgaccatct gccatcgctt tcccgtcgac gccatcgtca tcgccagccg
     1501 ctacgacctg acggtcgaag tggaaatgat ccgagcgatc aaggacaacg tctttctctc
     1561 catcatcccg gtcatcgtct accacccgga cccgccgacc gacgtcatgg tggccgccta
     1621 cgaacagggg gcggaggact tcatctacgg ggaatggcgg gacaagctcg tcaaagtgcg
     1681 gatcaggacc gcgatcgagc gcagccgccg cgacctgtcg atcaacccct cgacgcggct
     1741 gcccgggccc gcaatcatcg agcgggaaat ccagcgcatg ctcgagatgc aggcggagtt
     1801 cgcggtcggc tatgtcgacc tggataattt caaagcctac aacgactact acggatacta
     1861 ccggggggac aaggtcatcc agctcaccgc gcgcgtggtc aaggacgccg tctgcgacct
     1921 gtgcgacggc gggtttgtcg gacacatcgc cggcgacgat ttcatcttca tggtcccggt
     1981 cgaccaggtg gagcgcgtgt gccaggcggt gatccggaca ttcgacacgc tcattcggtt
     2041 ttgctacgag gaggaggatc gggagcgggg gtacatcacc accaagagtc ggcgcggcga
     2101 gattgagaat ttcccgctgc tcacggtgtc gatcgcggtg ctgctcaata ggaacgggac
     2161 tttcgcgcac atcggggaga tggcgaaaat gctggccgac ctcaagaaag cgaccaagca
     2221 gaaagccgga tcgaattaca tggtggagcg gcgcgggaag tactaggcgg cgggagccgc
     2281 ccgcaatcgt cgggcggcgt cggatctgtg tcaccgccgg cgactggccg cgccggatga
     2341 ataagctgcg cggtcagctg ccgaggagtc gggcatcctg caaactatac gcgcaccggc
     2401 ggcgactatc tgcggcccgg ccggcgaggg gaaggaaacc cgccggcatt ggcgggacgc
     2461 cgaccggaag agtcgttgac aaacttgtcg gcgattggta ggttgcggcg gacatggcgg
     2521 cacccggcag ccgggaaaca gccgccgcag ggttggtgtc ggtttcgccg aaccgctccg
     2581 agattcactc agcccggctg cggtctgcgc cgcgggacat gcttcggagc cggccgcgcc
     2641 tctcgaaccc cggggggccg gcgcgcggcc ggcggcgccg gtacgaaagt tcgcggggcg
     2701 tctcgcccag gaggacagct catgtggaag cgtctgatcg ttctctcagc aggattgctg
     2761 attggcgtgc cttggtcggg atgcgggtcg gggccggagg agtcggctga cgtcggaccg
     2821 gagccgccct cccgcgtcgt gggaatgttg cagggcggtt tgacctggcc ggcgagtatc
     2881 agtgtctggt tcgaacaccc ggatccggcg tcgatagatt cgttcctgat ctatcgcaag
     2941 atcggcgacg gctcattcgc cccggtgaag gtgaaaacgc tgcagccggc cgccgggacc
     3001 ggcaatccaa aggggggcga acccggggat gttcc
//
LOCUS       DAOPOE010000181         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_37986_length_2860_cov_4.501604, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000181 DAOPOE010000000
VERSION     DAOPOE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37986_length_2860_cov_4.501604"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKM94_13950"
     CDS             complement(<1..479)
                     /locus_tag="PKM94_13950"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOD67781.1"
                     /translation="MRKYSPYALFLLLAVFTAILYVNGFAPLDHLQRSINDFLCAFTA
                     DDAPRSNVATVTIDGRAQSKFGNWPWDHDLIADLVAATGAGDPKTVVVALDLSEDLHQ
                     DSSGYTDILAEQLRWVPNVVLPYDIAVAAYRGSATSNPDYLFVHSVSVDNPLGLMDE"
     gene            complement(490..1218)
                     /locus_tag="PKM94_13955"
     CDS             complement(490..1218)
                     /locus_tag="PKM94_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOD67782.1"
                     /translation="MRLALISDIHGNLEALTAVLRDIEDKGADEIACLGDVVGYGCDP
                     QACLELVARTCRVKLLGNHDAAALGRTPIEGYNSTARITAEWTRTVLSDRDRACLESF
                     TLQHRALGALLVHASPCRPDQWPYILAPDEAAEAFGAFTEPICFHGHTHIPAIFTEIP
                     GAFPARRVAHDIDPHEESRYLINVGSVGQPRDRDPRACWTLYDAAEGAIAFRRVAYDI
                     ARTQSKMSEAHLPDTLVERLAAGR"
     gene            complement(1221..1919)
                     /locus_tag="PKM94_13960"
     CDS             complement(1221..1919)
                     /locus_tag="PKM94_13960"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOD67783.1"
                     /translation="MPEIVVKYDDKVIERIVTEKKRLSIGRTNENDIVLENRGVSRRH
                     AMIEFNDNAAVVIDNESLNGTFVNNRRISEEVLRDSDVITIGKYNLVYRSQSQQTAGD
                     DLSTMDGTMVLNTKQQKQLIENDRRERQLVERYGGSVIVGESNADFSEYRLDRDVITI
                     GRAKFVHIRAKGLFLSGIQAKIVRDTGGFIIVNLGRKGKVRVNGETIDRAVLKNGDLI
                     EVGKSTFKFIEGRA"
     gene            2034..>2860
                     /gene="dusB"
                     /locus_tag="PKM94_13965"
     CDS             2034..>2860
                     /gene="dusB"
                     /locus_tag="PKM94_13965"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOD67784.1"
                     /translation="MQIGNLNLGGAVVAAPLAGVSDRAYRRLARRGGAALVFTEMIST
                     DGLVRDNAKTLEMIAFGPDERPIGVQLFGADPAVVEEAAARVAERFQPDLIDLNFGCP
                     VRKVVGRNGGAAMLKDPDLAGQVMAAAVRGAKTVQGNGGQGIPVTVKMRTGWDETRPV
                     YLDLAAVAEAAGVAAVTLHARSRSRGFAGKADWEAIARLKQAVGITVIGNGDVTTPED
                     ARRLMRDTGCDGVMVGRAAMGDPWVFGRIERYLATGERVPEPSAAERINWMREHVAMA
                     "
BASE COUNT          437 a          812 c         1101 g          510 t
ORIGIN      
        1 tgctcgtcca tgaggccgag ggggttgtcg acggagacgg agtggacgaa gaggtagtcg
       61 gggttgctcg tcgcgctgcc gcggtaggcg gcgacggcga tgtcgtaggg gaggacgacg
      121 ttggggaccc agcgcagctg ctcggcgagg atgtcggtgt agccggagga atcctggtgg
      181 aggtcctcgg agaggtcgag ggcgacgacg acggttttgg ggtcgccggc gccggtggcg
      241 gccacgaggt cggcgatgag gtcgtggtcc cagggccagt tgccgaattt cgactgggcg
      301 cggccgtcga tcgtcacggt ggcgacgttg gagcgcggcg catcgtcggc agtgaaggcg
      361 cacaggaagt cgttgatcga ccgctggagg tggtcaaggg gcgcgaagcc gttgacatag
      421 agaatggccg tgaagacagc cagcagcaaa aacagcgcgt agggcgagta ctttctcata
      481 atgctctctc tatcttcctg cagcgagacg ctccaccagg gtatccggca agtgcgcctc
      541 ggacattttc gactgcgttc gcgcgatatc gtacgcgacg cggcggaagg cgatggcgcc
      601 ctcggccgca tcgtagaggg tccagcaggc ccgcggatcg cggtcgcggg gctggccgac
      661 cgatccgacg ttgatgaggt agcggctttc ctcgtggggg tcgatgtcgt gggcgacccg
      721 gcgggccggg aaggcgcccg ggatttcggt gaagatcgcc ggaatgtgcg tgtggccgtg
      781 gaagcagatc ggttcggtga aggcgccgaa ggcctcggcc gcctcgtccg gggcgaggat
      841 gtagggccat tggtcgggcc ggcaggggga ggcgtggacg agcagggcgc cgagggcgcg
      901 gtgctgcagg gtgaagcttt ccaggcaggc gcggtcgcgg tcgctcagca cggtgcgcgt
      961 ccactcggcg gtgatacgcg cggtggagtt gtagccctcg atgggcgtgc gcccgagcgc
     1021 cgccgcgtcg tggttgccca gcagtttgac ccggcaggtg cgggcgacga gctcgagaca
     1081 ggcctgcggg tcacagccgt agccgaccac gtccccgagg cacgcgatct cgtcggcccc
     1141 cttatcctcg atgtcgcgca ggacggcggt gagcgcctcg aggttgccgt ggatgtccga
     1201 tatcagcgcc agccgcatga tcaggctcgt ccctcgatga atttgaaagt actcttgccg
     1261 acctcgatga ggtcgccgtt tttcaggacc gcgcggtcga tggtctcgcc gttgacccgg
     1321 acctttccct tgcggccgag gttgacgatg atgaagccgc cggtgtcgcg gacgattttc
     1381 gcctggatgc cggagaggaa gaggcccttg gcgcggatgt ggacgaactt ggcgcggccg
     1441 atggtgatga cgtcccggtc gaggcggtac tcggagaagt cggcgttgga ctcgccgacg
     1501 atgacggagc cgccgtatcg ctcgaccagt tggcgctcgc ggcggtcgtt ctcgatgagc
     1561 tgtttctgct gcttggtgtt gaggaccatg gtgccgtcca tggtgctgag gtcgtcgccg
     1621 gcggtctgct gcgactggct gcggtagacc aggttgtact tgccgatggt gatgacgtcg
     1681 gagtcgcgca gcacctcctc gctgatgcgg cggttgttga cgaaggtgcc gttgagggat
     1741 tcgttgtcga tgacgacggc ggcgttgtcg ttgaactcga tcatggcgtg gcggcgggag
     1801 acgccgcggt tttccaggac gatgtcgttt tcgttggtgc ggcctatgga aaggcgcttc
     1861 ttctcggtga cgatgcgctc gatcaccttg tcatcgtatt tgaccacaat ttccggcatc
     1921 gggaacctct ctgtctgcgg gctgcttatc tcatcggtag ctgaagccga accgccgccc
     1981 gcccggcgcg cgttccttgc ggcgatccga ggactttgct atatattgtc ggcatgcaga
     2041 taggaaactt aaacctgggc ggggccgtgg tggcggcgcc cctggccggg gtctcggacc
     2101 gggcctaccg gcggctggcc cgacggggcg gggccgcgct cgtattcacc gagatgatct
     2161 cgaccgacgg cctggtgcgg gacaacgcca agactctgga aatgatcgcc ttcggccccg
     2221 atgaacggcc gatcggggtg cagcttttcg gagccgatcc ggcggtggtc gaggaggcgg
     2281 ccgcccgcgt ggccgagcgg tttcagcccg atctgatcga cctcaatttc ggctgcccgg
     2341 tgcggaaagt cgtggggcgc aacggggggg cggcgatgct caaagacccg gatctcgccg
     2401 gacaggtgat ggcggctgcc gtgcgcgggg cgaagacggt tcaaggcaat ggcggacaag
     2461 gaattccggt gaccgtgaaa atgcggaccg ggtgggacga gacccgtccg gtctacctgg
     2521 atctggccgc ggtggccgag gccgccggag tggccgccgt caccctccat gcccgcagcc
     2581 gctcgcgcgg gttcgccggc aaagcggact gggaggcgat cgcccggctc aagcaggccg
     2641 tgggaatcac ggtgatcggc aacggcgacg tcacgacacc ggaggacgcc cgacggctca
     2701 tgcgggatac ggggtgcgac ggcgtgatgg tggggcgggc ggcgatgggg gatccctggg
     2761 tgttcgggcg gatcgagcgc tacctggcca ccggggagcg cgtgcccgag ccctcggcgg
     2821 ccgagcgcat caactggatg cgggagcacg tggcgatggc
//
LOCUS       DAOPOE010000182         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_41011_length_2700_cov_6.271078, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000182 DAOPOE010000000
VERSION     DAOPOE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41011_length_2700_cov_6.271078"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKM94_13970"
     CDS             complement(<1..322)
                     /locus_tag="PKM94_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67785.1"
                     /translation="MKVLVVALQKDNKVASATYELIEAAKGLGGQINSVIMAENAEPM
                     AQEIAGRGGAANGGKVIAVSDSHLRHFNDQLYTAALSELIARHTPDIVMGPATFYGKA
                     LFARL"
     gene            577..2574
                     /locus_tag="PKM94_13975"
     CDS             577..2574
                     /locus_tag="PKM94_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOD67786.1"
                     /translation="MKQLPSTAVQRSWYAAPISRFLTSSEDRVLAELLRNSTFTILQE
                     QRDAWLAELAILRRALPDRSGMLHLEFSIPRMGSRADAVVLIDGIVFVMEFKIGAKVY
                     SPADIDQACDYAVDLKYFHEGSHGLAVIPILVASEASQAVTDIRPHARVPGLYEPVCT
                     NASGLQPLVHRLLETVPTASVDPAAWGASRYRPTPTIIEAARALYAGHHVAEISRHDA
                     GAINLGETSDRLLAIIEETRSRKQKAICFVTGVPGAGKTLVGLNIATKYNDPGSELHS
                     TYLSGNGPLVAILREALARDMVRREKMQGRKLRKTEATTRVKAFIQNVHHFRDEYIRD
                     QRAPVDHVALFDEAQRAWDRLQTAAFMKKRSKQAGFPYSEPEFLISCMDRRDDWAVIV
                     CLVGGGQEIHRGEAGIGEWIQAVRRSFDHWHLHISDSLFDTEYAAGRVLEDIKGRARV
                     NFVPDLHLGVSIRSFRSEKVSAFVKQILDCEAAAARGLLHDFIDQYPIRLTRDLDRAK
                     QWLRDQAVGNERYGIVVSSQAERLKPLAMDVRTPVNPVKWFLDGKDDVRSSYYLEDVA
                     TEFHVQGLELDWTCVSWDGDFRYDNRRWQHFSFRGTTWQRVKQEQRKVYLKNAYRVLL
                     TRARQGMVIFVPRGSGHDPTRDPRFYDSTYRYLQEIGLPEL"
BASE COUNT          490 a          896 c          789 g          525 t
ORIGIN      
        1 ccagccgcgc aaacagcgct ttgccgtaga acgtcgccgg ccccatcacg atatccggcg
       61 tgtgccgggc gatcagctcg gacagggcgg ccgtgtagag ctggtcgttg aaatggcgaa
      121 ggtgcgaatc gctgactgcg attaccttgc cgccgttggc cgcgcccccg cggccggcga
      181 tttcctgcgc catcggctcg gcgttttcgg ccatgatgac cgagttgatt tggccgccga
      241 ggcctttggc ggcttcgatg agctcatagg tagccgacgc aaccttattg tctttctgga
      301 gcgctacaac caataccttc atgccgactc ctctaagtta gccaagtcac tggagggaat
      361 atatggggcg gcaatttcga gagcaactct tttgcacggc ggggcaactg aaccgagggg
      421 cgattggcac cgcggctggc atgcgtgccg caatctcaaa cggccgaccg gaatcattct
      481 cgccggccga aaaaacttgc ctccaccgca cccaccccca tacttgtcga ttggcgagta
      541 ccgaaagcga cgagtcttgg tttgggtccg gcacagatga aacagcttcc ctctacagcg
      601 gtgcagcggt cgtggtatgc ggcgcccatt tctcggtttt tgacttcctc cgaggaccgg
      661 gttctcgcgg agctcctacg caactccact ttcactatcc tgcaggagca gcgtgatgcc
      721 tggctggccg aactggctat ccttcgccgt gccctccccg accgctccgg catgctccat
      781 ctcgagttct ccatcccccg aatgggcagc cgcgccgatg cggttgtgct gatcgacggg
      841 atcgtgttcg ttatggagtt caagatcggc gcgaaagtgt actcccctgc ggacatcgac
      901 caggcgtgtg actacgccgt cgacctgaag tacttccacg aaggcagcca tggcctcgcc
      961 gtgatcccca tcctcgttgc gtccgaggcg tcccaagcgg ttaccgacat tcgcccccac
     1021 gcgcgggtac ccgggcttta cgaaccggtt tgcaccaacg catcggggct tcagcctctc
     1081 gttcatcggc tgctcgaaac ggtccccacg gcatcggtgg atccggccgc ctggggcgcg
     1141 agtcgttatc ggccgacgcc cacgattatc gaggccgccc gggcgctcta tgccggccac
     1201 cacgtcgccg agatctctcg tcacgatgcc ggagccatta acctgggtga gacctccgac
     1261 cggttgctcg ccattatcga agagactcgg tcccgaaagc agaaagccat ctgtttcgtc
     1321 accggcgtcc ccggcgccgg caagaccctg gtgggcctca acatcgcgac gaaatacaac
     1381 gacccgggca gtgaactcca cagtacctac ctatccggca atggcccgct cgttgccatt
     1441 ctccgcgagg ccctcgcccg tgacatggtg cgccgtgaga agatgcaggg acggaaattg
     1501 cggaaaacgg aagcgacgac aagggtcaag gccttcattc agaacgtcca ccacttccgc
     1561 gatgagtaca tccgggatca gcgcgccccc gtcgatcatg tcgccttgtt cgatgaggcg
     1621 cagcgggcct gggaccgcct gcagactgcc gcgttcatga agaagcgctc caaacaggcc
     1681 ggcttcccct actccgagcc ggaatttctc atctcctgca tggatcgccg cgacgattgg
     1741 gccgttatcg tctgccttgt cggcggcggt caggaaatcc accgcgggga ggccggcatc
     1801 ggcgagtgga ttcaggcggt ccggcgctcc ttcgatcatt ggcatctcca catctccgac
     1861 agtctcttcg acaccgagta cgccgccggg cgtgttctcg aagacatcaa ggggcgcgcg
     1921 cgtgtgaact tcgtcccgga cctccacctg ggtgtctcca tccggtcctt ccggtccgag
     1981 aaagtctcgg ctttcgtgaa acagatactt gactgtgagg cggcggccgc ccgcggtttg
     2041 ctccacgact tcatcgacca gtatccgatc cgcctcaccc gcgacctcga ccgcgccaag
     2101 cagtggttgc gcgatcaggc ggtcggcaat gaacgctacg gcatcgtcgt gtcctctcag
     2161 gccgaacgcc tgaaaccgct cgccatggat gtacgcacac ccgtcaaccc ggtgaagtgg
     2221 ttcctcgatg gcaaagacga cgtgcgttcc tcgtactacc tcgaagatgt agcgacggag
     2281 tttcacgtcc agggactcga gctcgattgg acttgcgtct cctgggacgg ggatttccgg
     2341 tacgacaatc ggaggtggca gcatttctcg ttccgcggca ccacgtggca gcgagtcaag
     2401 caggagcagc gcaaggtcta cctcaaaaat gcctaccgcg tcctcctcac ccgcgcccga
     2461 cagggcatgg tgatcttcgt cccccgaggc tccggccatg acccgaccag agatcctcgc
     2521 ttctacgaca gcacgtaccg gtaccttcag gagatcggac tgccggagtt atagaactcc
     2581 gccaaccgtc atccaccgct tatcccggct cgggtcgcat cactggcccg cacgcgtcgt
     2641 cggtcggggc acgccgttga cccggggaca tcgtcggtcg gggcacgccg ttgaccggcg
//
LOCUS       DAOPOE010000183         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_42684_length_2620_cov_5.883431, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000183 DAOPOE010000000
VERSION     DAOPOE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42684_length_2620_cov_5.883431"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKM94_13980"
     CDS             <1..671
                     /locus_tag="PKM94_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67787.1"
                     /translation="DGATMVTNPPLPGIIPGTISHLFVSGAVVRPISDFEVGLDLPPS
                     LGAGVSYQATDRLVLALDGEYTFWSRFDGLDFAFSNAVGLAGPADTVPLAREFFTADL
                     SAPVDWDNSVKLMLGASYDFPEFLAPEVGLTLVGGLSWDQSPARNEWRLTPQLLDTGN
                     KFGFSGGGIVHLDRWDLGLVTSYTRLQHQRAWDLDMDEAFTSFPGYYQPQTFETILSF
                     NYRF"
     gene            692..2017
                     /locus_tag="PKM94_13985"
     CDS             692..2017
                     /locus_tag="PKM94_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOD67788.1"
                     /translation="MTRYLLAALILVLTLATGCSDRGDNTGPVYDGESGVLTADHMFF
                     QELSTQIRNQGRWMLYLKAWVPKYNEWGPPPNYHPDHPLPLLVLLPPEDADEWYYFHH
                     GLSQVANELMAEGLLEPMAIFVPQNDPVLGGYWWAGSGGGCGNYDTLVGGTLIDHIRE
                     NALGPGAFDTAQCMTAIGGIGTGAYGAVRAALKHPGRFGSISVINGPMDFDGLDGQGG
                     LIPLFAKAIAEQSGVPFATKWDTSSYNEISRLLTGGAMAFSPHDTLVYPVITYTQTGP
                     RVACPDSAKITGTDTWVRTRYKIAGTETLLPDLISAADFSFQVHLPFDRFGRPYSAPN
                     EPIWDLWLRNSPDSMIYNAESQGRAFPNTHFWVAYSREDDPLGYNVMTRDFAKLLQKY
                     RYDPEIYYFGGYEGNPGWGDEYLTHLLREVLIFHSNCFRQARQNQQGGN"
     gene            2091..>2620
                     /locus_tag="PKM94_13990"
     CDS             2091..>2620
                     /locus_tag="PKM94_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOD67789.1"
                     /translation="MAETTPYDLLSVGAHPDDVEVGTGGVLIDAARRGYKVAVAILTE
                     GELGTGGTAEIRRREVEEAAGILGAEVVARFDWGDTRLEDTYEHRRALAEVLRRTRPR
                     IVLAPYPHVGHGRRQSHPDHVAAGIIAINAANLAALKKADVPGEPHLVTRIFHYFLPP
                     GVSPNFVVDITPHFDR"
BASE COUNT          445 a          920 c          802 g          453 t
ORIGIN      
        1 cggacggcgc gaccatggtg accaacccgc cgctgcccgg gatcatcccc ggcaccatca
       61 gccacctgtt cgtctcgggg gccgtggtgc ggccgatctc cgatttcgag gtgggtctcg
      121 atctgccgcc gagcctgggc gccggcgtgt cctaccaggc gaccgaccgg ctggtgctgg
      181 cgctcgacgg cgagtacacg ttctggtcgc gctttgacgg actcgacttc gcgttctcga
      241 acgccgtggg cctggccggt ccggccgaca cggtgccgct ggcccgcgag ttcttcaccg
      301 ccgacctcag cgcaccggtc gactgggaca attcggtgaa gctgatgctc ggagcctcat
      361 atgactttcc ggaattcctg gcgcccgagg tggggttgac gctggtgggc gggctgtcgt
      421 gggatcagtc gcccgcgcgc aacgagtggc gcctgacccc gcaactgctc gacaccggca
      481 ataagttcgg cttctccggc ggcggcatcg tgcacctcga ccgctgggat ctcggcctcg
      541 tcacctcgta cacacgtctg cagcaccagc gggcctggga ccttgacatg gacgaagctt
      601 tcaccagctt ccccggctac taccagccgc agacttttga aaccatcctc tcgttcaact
      661 accggttcta gggaggtcgg agatgcaagc aatgacgaga tacctactgg ctgctttgat
      721 tctggtgctc acgctggcga cgggctgctc cgaccggggt gacaacaccg gacctgtata
      781 tgacggcgag agcggcgtgc tgaccgccga tcacatgttc ttccaggaac tgtccaccca
      841 gatccgcaac cagggtcgtt ggatgctcta cctgaaagcc tgggtgccga agtacaatga
      901 gtggggcccg ccgccgaact accacccgga ccacccgctt cccctcctgg tgctcctgcc
      961 gccggaagac gccgacgagt ggtactattt ccaccacggc ctctcgcagg tggccaatga
     1021 gttgatggcg gaagggctcc ttgagccgat ggccatcttc gtcccccaga acgacccggt
     1081 tctgggcggc tactggtggg cgggatcggg cggcggctgc ggcaattatg acactctggt
     1141 cggcggcacg ctgatcgacc acatccggga gaatgcgctc ggccccggcg ctttcgacac
     1201 ggcgcagtgc atgaccgcca tcggcgggat cggcaccggc gcctatggcg ccgtccgcgc
     1261 cgccctcaaa caccccggac gcttcggatc catcagcgtc atcaacggcc cgatggattt
     1321 cgacggcctc gacggccagg gcggcctgat ccccctgttc gcaaaggcca tcgccgaaca
     1381 gtccggcgta cccttcgcca ctaagtggga cacctccagc tacaacgaga tcagccgtct
     1441 cctcaccggc ggcgctatgg ctttttcccc gcacgacacc ctcgtgtacc cggtgatcac
     1501 gtacacccag actgggccca gagtcgcctg tcccgactcc gcgaagatca cggggaccga
     1561 tacgtgggtg cgcacccgct acaaaattgc aggtacggag acgctcctgc ccgatctcat
     1621 cagcgccgcc gatttctcgt ttcaggtcca cctcccgttt gatcggttcg gccggcccta
     1681 cagcgcgccc aacgaaccga tctgggacct gtggctgcgc aacagcccgg actcgatgat
     1741 ctacaacgcc gaaagccagg ggcgggcctt tcccaacacc cacttctggg tggcctactc
     1801 ccgggaggac gaccccctcg ggtacaatgt catgacccgg gacttcgcca agctgctgca
     1861 gaagtaccgg tatgatcccg agatttacta cttcggaggc tacgagggca acccggggtg
     1921 gggcgacgag tacctcaccc acctgctgcg cgaagtgctc atcttccact cgaactgctt
     1981 ccgtcaggcc cgccagaacc agcagggcgg gaactgaccc gatttgaccg gcacatgcgc
     2041 ccggagaact cgtttctccg ggcttttttg tagaaggaaa ggacaggcgg atggctgaga
     2101 caacccccta cgatctgctt tccgtgggag cacaccccga cgacgtcgag gtggggaccg
     2161 gcggcgtgct gatcgacgcc gcccggcggg gctacaaagt cgccgtcgcc attctcaccg
     2221 agggggaact gggcaccggc ggcaccgccg agatccggcg gcgggaggtg gaggaggcgg
     2281 ccgggatcct gggcgccgag gtcgtcgccc gctttgactg gggggacacc cggctggagg
     2341 acacctatga acaccggcgg gccctggcgg aggttctgcg gcgcacccgt ccccgcatcg
     2401 tcctggcccc gtacccgcac gtcggccacg gccggcgcca gtcgcacccg gatcatgtcg
     2461 ccgccggcat catcgccatc aacgccgcca acctcgcggc gctgaagaaa gccgacgtcc
     2521 ccggcgaacc ccacctggtc acgcgcatct tccactactt cctgcccccc ggcgtttccc
     2581 ccaacttcgt cgtcgacatc acgccgcact tcgaccgctg
//
LOCUS       DAOPOE010000184         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_47366_length_2424_cov_4.970030, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000184 DAOPOE010000000
VERSION     DAOPOE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47366_length_2424_cov_4.970030"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKM94_13995"
     CDS             complement(<1..913)
                     /locus_tag="PKM94_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOD67790.1"
                     /translation="MTRVTFISLLLLALAGSAAAVAPHPEAIEKWKAEGVWKEKVDAW
                     QAFKARGGCAPNETPIWQPGKEPRAMAAQAGPDTVKVIVLLVEFTDNRMSGGYANGTN
                     AVFDSILFSDSTIDTAGAIINPTGSMTDYYREVTYGGLYLAGDIYGPYTAPNTYAYYV
                     GSDDGLSRSAELVYDLTNLADPDIDYTQYTNGDNDVDAVIVIHAGRGAEEGIFGIWSH
                     KASINATRDGVQVKYYTMNPEEAGDHLSAIGVFCHEFGHVLGLPDLYDVTYAEGSSGA
                     GHWSLMGNGNYLFGSKRPAHLDGWCKAQ"
     gene            complement(1014..>2424)
                     /locus_tag="PKM94_14000"
     CDS             complement(1014..>2424)
                     /locus_tag="PKM94_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOD67791.1"
                     /translation="DLAITLRNPAANIVRATATLAAPAASGVTVESGEVIYLFGGDLL
                     SAVNGAPLTATFSDTLYHGQTAAFTLCVRYEYNLAEHTDTLTFTLPVGVAPAGSFATH
                     RTAQMDLTVSDFGMFGLAPGSIYNVRGEGFTVGGSDNLLYEAGVIIGRNALQLSTGVR
                     GADGGFAASDFLPVAPLSEEWVDLDGGRHRTAEYDDRGAEISIPITVRQETVSYPGMD
                     HSSLLVVKFRLKNTSIEPLTNLRFGLLADYDLGGAETVAYDANLNMIVTAGADGSRAA
                     LMGLDGAVSFSAFANQAGAKTGFSGGELYDILSRSGVDLGEASGDDRFVIVGAGPFSI
                     PPRDSVTIALAFVAGLAEADFEAAAAAARRMYDEATAAADERPDGLPAAFELHQNYPN
                     PFNPTTRIRFDLPRAAEVSLKIYNTLGQEVTVLHEGPLAPGRHAVDWEAAGDLASGVY
                     FYRLTVGDTQQTRKMVLLK"
BASE COUNT          398 a          810 c          770 g          446 t
ORIGIN      
        1 cctgcgcctt gcaccacccg tcgaggtgcg ccggccgctt ggacccgaac aggtagttcc
       61 cgttccccat gagtgaccag tgccccgctc cgctggaacc ctcggcgtag gtgacgtcat
      121 agaggtccgg cagccccagc acgtggccga actcgtggca gaagaccccg atggcggaga
      181 ggtggtcccc cgcttcctcc gggttcatcg tgtagtactt cacctgcacg ccgtcgcgcg
      241 tggcgttgat ggacgccttg tgcgaccaga tgccgaagat tccttcctcg gcgccgcggc
      301 ccgcgtggat gacgataact gcgtcgacgt cgttgtcgcc gttggtgtac tgggtgtaat
      361 cgatgtccgg gtcggcgagg ttcgtgagat catagacaag ctcggcgctg cgcgagaggc
      421 cgtcgtcgga gccgacgtag taggcgtagg tattcggcgc cgtgtacgga ccgtagatgt
      481 ccccggcgag gtacaggccc ccataggtga cttcgcggta gtagtcggtc atcgatccgg
      541 tcgggttgat gattgcgccg gcggtgtcga tggtgctgtc cgaaaagagg atcgagtcga
      601 acacggcgtt ggtgccgttg gcgtacccgc cgctcatgcg gttgtcggtg aattccacca
      661 ggagcacgat cactttcacc gtgtccggac cggcctgggc ggccatcgcc cgcggctcct
      721 tgccgggctg ccagatcggt gtctcattgg gagcgcagcc gccgcgcgcc ttgaacgcct
      781 gccaagcgtc gaccttctcc ttccacactc cctcggcttt ccatttttcg atcgcctccg
      841 ggtgcggggc cacagctgcc gccgaaccgg ccagggcgag cagcaacagg gaaatgaacg
      901 tcacacgtgt catacgggga accttcgctg tccttatcgc ggtgtatgaa ttctacttac
      961 cctgtaaact ccgccgcacg ccgttcgttt cccggaaaat cgatattccg ggactatttt
     1021 agaagcacca tcttccgcgt ctgctgggtg tcgcccacgg tcaggcggta gaagtacacg
     1081 ccgctggcca gatcccccgc cgcttcccag tcgaccgcgt gccgcccggg ggccagcggc
     1141 ccttcgtgca gcaccgtcac ttcctgtccc agcgtgttgt agatcttcag cgacacctcg
     1201 gcggccctcg gcagatcgaa acgaatccgc gtggtcgggt tgaaggggtt cgggtagttc
     1261 tggtgcagtt cgaaggctgc cggcagaccg tccggccgct catccgctgc cgctgtcgcc
     1321 tcgtcgtaca tccggcgggc cgctgccgcc gcggcttcga aatccgcctc cgcgaggcct
     1381 gcgacaaagg ccagagctat ggtgaccgaa tcccttggcg ggatcgaaaa cggtccggcg
     1441 ccgacgatca caaaacgatc gtcaccggac gcctcgccga gatccacccc ggaccgggat
     1501 agtatatcat ataactcccc gcccgaaaag cccgtttttg cgccggcttg gttggcgaag
     1561 gcgctgaacg aaaccgcccc gtccagcccc atcagcgccg cccgggaacc atcggcgccc
     1621 gccgtcacaa tcatgttgag attcgcatcg taggccaccg tctccgcgcc gcccaggtcg
     1681 tagtcggcca agaggccgaa gcggaggttg gtgagcggtt cgatgctcgt gtttttgaga
     1741 cgaaacttga caaccagcag gctgctgtgg tccatccccg ggtagctcac cgtttcctgc
     1801 cggaccgtga tgggaatcga gatctccgcc ccgcggtcgt cgtactcggc cgtccggtgc
     1861 cggccgccgt ccaggtccac ccactcctcg ctgagcggcg cgacagggag gaaatcggag
     1921 gcggcgaacc cgccgtcggc gccccgcacg ccggtggaga gctgaagggc gttgcgcccg
     1981 atgatcacgc cggcctcgta cagcaggttg tcgctgccgc cgactgtgaa cccctccccc
     2041 cgcacattat atatcgagcc gggcgccagc ccgaacatgc cgaaatccga caccgtcaag
     2101 tccatctgcg ctgtccggtg ggtggcgaag ctgcccgccg gcgccacccc gaccgggagc
     2161 gtgaaggtca gtgtgtccgt gtgctctgcg agattgtact cgtaccggac gcagagagtg
     2221 aaggccgccg tctggccatg atagagcgta tcgctgaagg tcgctgtcaa cggcgccccg
     2281 ttgaccgcgc tcagcaggtc gccgccgaac aggtagatca cttcccccga ctcgactgtg
     2341 acgcccgagg ccgccggggc cgcgagagtg gccgtcgccc ggacaatatt ggccgccggg
     2401 ttgcgcagcg tgatcgccag gtcg
//
LOCUS       DAOPOE010000185         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_50207_length_2318_cov_5.529828, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000185 DAOPOE010000000
VERSION     DAOPOE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50207_length_2318_cov_5.529828"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKM94_14005"
     CDS             <1..1300
                     /locus_tag="PKM94_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD67792.1"
                     /translation="VMGVVLIPAAVFLLLADTGIGRALGRASRGVKAALAVGLLAAVL
                     AVFRHAMATWSFFRLAFLPPTPNRFTVGGYTLFSDTHLLDFVNQALVLFPGLLVCVPA
                     LMPALRRRGAGKRAVMFLALFGLGAAGAVFIFDPKLGMPRDWDLFAFAGVPIALVCLY
                     PLVRRLRAVAVAAPAVLLALGLNVIILGGRAALQTAPEASADQFQQLMNWDLPKSQIS
                     WTILVSYYLDRGDSTQAQELYDAWLARFPGYLWNRDGLDRLHEGKYPEALALFRQVMQ
                     ANPAFSPAYGNLGATFLAAGRLDSAEIYIDIALGMSPHQPVNMVNRGKLLWLQGDKDG
                     AVKWYRRALAMDSTEHRAALGLADYYRDKRDRDLYFRYLELANAYGGAPPGRMAELGT
                     YFLATGRRTQGEECIRRAVEAGLDSATTNRLRELYPDLGL"
     gene            complement(1325..2158)
                     /gene="panB"
                     /locus_tag="PKM94_14010"
     CDS             complement(1325..2158)
                     /gene="panB"
                     /locus_tag="PKM94_14010"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOD67793.1"
                     /translation="MSKVSQTKRTTIQTFITKKAKGEKITVLTAYDAFTAALLDAAGI
                     DAVLVGDSAANVLHGFDSTIPITTEIMVAHTAAVARGAKRALVIADMPFLSFQPSEET
                     AIRNAGGFLKAGAQAVKIEGGLEIAPLAAKLVGFGIPVMGHIGLTPQSIHRFGGPKVQ
                     GREERSRTYLRESALALEEAGCFSIVLELMQADVAREITESLHTAATIGIGAGAHCDG
                     QVLVINDMLGLHEEGFRPKFLREYADLKTIIADAVRRYIEDVKSGDFPGDDESFGPQP
                     H"
BASE COUNT          336 a          725 c          810 g          447 t
ORIGIN      
        1 cgtcatgggg gtcgtgctga ttccggcggc cgtgttcctg ctcctggccg ataccgggat
       61 cggccgcgcg ctggggcgcg cctcgcgcgg cgtgaaagca gcgctcgcgg tgggtctgct
      121 ggcggccgta ctcgccgtct tccggcatgc gatggcgacg tggtctttct tccggctggc
      181 gtttttgccg ccgaccccga accggttcac cgtcggaggg tacaccctct tctccgacac
      241 gcacctgctc gactttgtca accaggcgct ggttctgttt ccggggctgt tggtgtgcgt
      301 gccggcgttg atgccggcgc tccggcggcg cggcgccggg aaacgggcgg tgatgtttct
      361 cgcgctcttt ggtctcggcg ccgccggcgc ggtgttcatc ttcgatccaa aactcgggat
      421 gccgcgcgac tgggatctgt tcgccttcgc gggcgtgccg atcgcgctcg tctgtctgta
      481 tccgctggtc cggcggctcc gcgcggtggc ggtcgcggcg ccggcggtgc tgctggcgct
      541 cggcctcaac gtcatcatcc tgggcggccg ggcggctctc cagacggcgc ccgaagcctc
      601 cgccgaccag ttccagcaac tcatgaactg ggatctgccg aagagccaga tcagttggac
      661 catcctcgtc tcctactacc ttgaccgggg agactcgacg caggcgcagg agttgtacga
      721 cgcctggctg gcccggtttc ccggctacct gtggaatcgc gacgggctgg atcggctcca
      781 cgaggggaaa tatccggagg cgctcgctct gtttcggcag gtgatgcagg ccaacccggc
      841 gttcagcccg gcctacggca atctcggtgc aacgttcctg gccgcgggtc ggctcgacag
      901 cgctgaaatc tatatcgaca ttgctctcgg aatgagtccg catcagccgg tcaacatggt
      961 gaaccgcggc aagctcctct ggttgcaggg cgacaaggat ggggcggtga agtggtatcg
     1021 ccgggcgctg gcgatggatt ccaccgagca tcgggccgcg ctcgggctgg ccgactacta
     1081 ccgcgacaag cgcgaccgcg atctctattt ccggtacctc gagctcgcca acgcgtatgg
     1141 cggggcgccg ccgggccgga tggcggaact gggaacatac tttctcgcga ccgggcggcg
     1201 gacacagggg gaggagtgca tccggcgcgc cgtggaggcc gggctggact ccgccacgac
     1261 gaaccgcttg cgggagctgt acccggacct gggcttgtag gggataaggc gcccgggcgt
     1321 ccgatcagtg cggctgcggg ccgaacgatt cgtcatcgcc ggggaaatcg ccggatttca
     1381 cgtcttcgat gtagcgcctg acggcgtcgg cgatgatcgt cttcaggtcc gcatattccc
     1441 gcaggaactt ggggcggaaa ccctcctcgt gcagaccgag catgtcgttg atcacgagca
     1501 cctggccgtc gcagtgcgcg ccggcgccga tgccgatcgt ggcggccgtg tggagtgact
     1561 cggtgatctc gcgcgcgacg tcggcctgca tcaattcgag cacgatcgag aagcagccgg
     1621 cctcttcgag agcgagcgcg gattctctca ggtaggtgcg ggagcgttcc tcgcgtccct
     1681 ggaccttggg gccgccgaag cggtggatcg actgcggggt gagcccgatg tgccccatga
     1741 cggggatgcc gaagccgacc agtttagccg ccagcggagc gatttccagc cccccctcga
     1801 tcttcaccgc ctgcgcaccc gccttgagaa acccgccggc gttgcggatc gccgtctcct
     1861 cggagggctg gaaagagagg aagggcatgt cggcgatcac cagggcgcgt ttcgccccgc
     1921 gggcgaccgc ggccgtgtgc gcgaccatga tctcggtcgt gatcggaatc gtggagtcga
     1981 aaccgtggag cacattcgcg gccgagtcgc cgaccagaac ggcgtcgata ccggccgcat
     2041 cgaggagggc ggccgtgaaa gcgtcgtacg cggtcaggac agtgatcttc tcgcctttgg
     2101 ccttcttcgt gatgaaggtc tgaatcgtgg tgcgcttggt ttggctgact ttcgacatgg
     2161 gcttgctcgc tcacccgatg cgggcggtgt cgccggacgg ggagtagtac ttcttgccgg
     2221 cgatcggttt gtgcagctgg tcgatgagat cgtcgaaatg gctcgggttg ttgacgaagt
     2281 cgatgttctc ggtctttacc accagcagcg gcgtctca
//
LOCUS       DAOPOE010000186         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_51172_length_2284_cov_6.279946, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000186 DAOPOE010000000
VERSION     DAOPOE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51172_length_2284_cov_6.279946"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1379)
                     /locus_tag="PKM94_14015"
     CDS             complement(312..1379)
                     /locus_tag="PKM94_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD67794.1"
                     /translation="MKRFWLAAIVALAALGLLAATGLAGKDTDERAWLGVYTQSVDYD
                     LADAFDLTVSYGAVINEVVEDSPADQAGLKNGDVIISLNGEKITDSDDLIDVLHEASP
                     GDRLELVVIRDGKEQSVSVVAGARSEEDDNLYGTFRSYFDGGNHSAYTLSGAKQTYLG
                     ITLNDLTDQLGGYFGVADGKGALVSEVAEDSPAERAGLKAGDVVIEIDGEKVADASEV
                     TEAIRDREAGDKVVITAIRNQRAENFTAELETRDSPFYGNFATIGPDLAILNVPKLRG
                     LSKGNYDGALEEYFNSEEFQREMKELQEQLGKLKFEFKDLDRLGDDRDELRKELEELR
                     RELQEMKAELKRELRDRQDRQ"
     gene            2164..2238
                     /locus_tag="PKM94_14020"
     tRNA            2164..2238
                     /locus_tag="PKM94_14020"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2196..2198,aa:Asn,seq:gtt)
BASE COUNT          418 a          671 c          762 g          433 t
ORIGIN      
        1 acacggcggt tggcgaagcg ctcaaaaaag ccggtcaggg cgagaccggg gcggtggagc
       61 tcgggatggt ggatgacttt gttcagcccg gccgtgctgc tgttcagcag ggtgagctcc
      121 agatcctggg tccgggtcgt atagagcttc tcgactgtga ttcccgacat ggcagggaca
      181 atatacaaaa cccggggagg gggcaactat gaaaaacagg ggcaaaatag accgccccgc
      241 cggaaggcgg ggcggttcga ggaggataaa aaggcactgt caggccccgg ggtaaagggg
      301 acccgtatca gttactgccg gtcctgccgg tctctcaatt cccgcttcag ttcggctttc
      361 atctcctgca gttcgcggcg cagctcttcc agctctttcc gcagctcgtc gcggtcgtcg
      421 ccgagccggt cgagatcctt gaactcgaat ttcagtttcc cgagctgttc ctgaagctcc
      481 ttcatctcgc gctgaaattc ctcggagttg aaatactcct ccagcgcgcc gtcgtagttc
      541 cccttggaga ggccgcgaag cttggggaca ttcagaatag ccaggtcggg gccgatagtc
      601 gcaaaattgc cgtaaaaagg actgtcgcgg gtctccagtt cggcggtgaa attctccgcc
      661 cgctggttcc ggatggccgt gatgaccacc ttatcgccgg cctcgcggtc ccggatcgcc
      721 tcggtcacct ccgatgcatc ggcgaccttc tcgccatcga tctcaataac cacatcgcct
      781 gctttgagcc ccgctctctc cgccggcgaa tcctccgcga cctcgctcac cagcgccccc
      841 ttgccgtcgg cgacgccgaa gtagccaccc aactggtcgg tgagatcatt cagggtgatt
      901 cccaggtagg tctgcttggc gcccgacaac gtgtacgcgg aatggttgcc gccgtcgaag
      961 taggagcgga aagtgccgta gaggttgtcg tcctcctccg accgcgcgcc cgcgacaacg
     1021 gagacactct gctctttgcc atcgcgaatg acgaccagtt cgaggcgatc gccgggcgag
     1081 gcttcgtgga ggacatcgat caaatcatcg gagtcggtga ttttttcgcc gttgagcgag
     1141 ataatgacat ccccgttctt caggccggcc tggtcggcgg gggaatcctc gaccacctcg
     1201 ttgatgacgg cgccgtagga cacggtgaga tcaaaagcgt cggccagatc gtagtcgacc
     1261 gactgcgtat agacccccag ccaggcgcgc tcatctgtat cttttccggc gagaccggtg
     1321 gcggcgagca ggccgagggc cgccagagcc acgatcgccg cgagccagaa tcgtttcata
     1381 gcgctatatc ctccatgggt cagactgttc aggttgctaa agcgatatac gcgggacgtc
     1441 cggcgaggtt tcacggggga gagaccgaga gggcggggcg ccggagcgca ggagatatgc
     1501 cgtcggcccg ggggtgccgg tgcagacggt gcgagccggg tcgcggagcc tctcccgtcc
     1561 tctcctctcc ccgccccgcc ccgtcccggc agagtctcat ggcggggcta ctcatatgcg
     1621 gggcaacaag ttgtacgtca cgcccccgac atccggccgg ctacgcccga ccgtctggat
     1681 tgaccggccg cgtggattgc accgggtcgc tcatgtgcag gggcacactg ccggggaacc
     1741 gatggcgcgc ggtccgcgaa tctatggctg ccttgcggac tcgcgcgggt ggggagaagg
     1801 ttggcagtcg caatgactac gccgaatccg tagtgatcgc tgtggcgatc ccgggcgatt
     1861 cggcgggagc agggacatcc ccgaggtgcg cgtggtccat gcctccgacg acgggccgcc
     1921 cggacgatgg gggccgggag gaccgatcgg gtgtgggcgg gtcggccgga agcggatcga
     1981 gtaagagcgg gtcggctaag agcggatcgg ctgagggcgg atcggctgag agcggatcgg
     2041 ctgagggcag atcgactgag agcggatcgg gtgaggggtg cgggaaaaag gagttgactt
     2101 tggcgggaaa aacggtatta atggtgagct tcgcctgcgg gcgaagcgcg gttttttgat
     2161 tactccggtg tagctcagtg gtagagcagc tgactgttaa tcagcgggcc gctggttcga
     2221 gtccagccac cggagccaaa attcaatccg ctgatttcag cggttcttgt ttaacgcaag
     2281 accc
//
LOCUS       DAOPOE010000187         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_56764_length_2114_cov_5.524526, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000187 DAOPOE010000000
VERSION     DAOPOE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56764_length_2114_cov_5.524526"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKM94_14025"
     CDS             <1..187
                     /locus_tag="PKM94_14025"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD67795.1"
                     /translation="SCERMTKSAECRGRLLPGYPADFVILSQDPTRVAPLRLYDTEIL
                     ATVLDGKVKYTAQGFSL"
     gene            complement(311..>2114)
                     /locus_tag="PKM94_14030"
     CDS             complement(311..>2114)
                     /locus_tag="PKM94_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD67796.1"
                     /translation="FYDYLVHLRFEEVFVTVSQLALDPRTAAELTEAALLHDLDAIYL
                     CDLEGNVLATGAAPAWEGLLPQFVQAEVRTLGDNPQTNYVLLLEPATREHGARHYYLQ
                     IANTLDRIAVLVADAQYYVEALRQTQIGYLVQRMAEEPGVVYIIYQSTDGIIFSSRRT
                     GPLLAIESDPFLTQALDADTIMHRTSEFQGERVLELVRPFATSEYPFGLFRVGLSLGR
                     YYSVSRRFDLQMAALSAALFGVLLFGLLYLAARRKRRELGRQYTDIKSLTDVIFDRMR
                     TGVAAVDDRGVITLANRAFEQIVGAAGVLGRNWDDAVTAPALARENLRRWMAAGEEKE
                     LTAAIGDREKTLVLAVSAIGQRRDEGMVIVLYDVTRLKEYERRALRRARLSELGDLAA
                     GVAHEIRNPLNTISIAAQRLAAEFTPTANREEFVGFTGQIRAETRRLNDIITRFLALA
                     REEKKGRQKVALDRLIADAVVFLGLEARKIGIALEAECEPGLTLEADPDELRQVLTNL
                     FNNAKEALAGQPGRIRVQARRAGDGRVALTFEDSGPGIAEDLRERVFAPYFTTKEAGT
                     GLGLPTVYRIVSDLGGEVRVERSDLGGARFVIAV"
BASE COUNT          331 a          807 c          642 g          334 t
ORIGIN      
        1 gtcctgcgag aggatgacga agtcggccga atgccgcggc cgcctcctcc ccggctaccc
       61 ggccgacttc gtcatcctct cgcaggaccc cacccgcgtc gccccgctgc gcctctacga
      121 caccgagatc ctcgcgaccg tgctggacgg aaaggtgaaa tacacggcgc aggggttttc
      181 attgtaggga agataacagc cgccttcggc cgctcaagcg cgccgcggac aatgccgcac
      241 ggtgctcccc tttagtccat tcggttggga ccacgaacag ccgcccccct gcggttgtgg
      301 atctcccccc tcagaccgcg atcacaaacc gcgccccgcc gagatcgctc cgctccaccc
      361 gtacctcccc gcccaggtcg ctgacaatgc ggtacacggt cggcagcccc agccccgtcc
      421 cggcctcctt ggtcgtgaaa tagggcgcga acacccgctc gcgcagatcc tcggcgatcc
      481 ccggcccgct gtcctcaaac gtgagtgcga cccggccgtc gccggcgcgc cgggcctgca
      541 cccggatccg ccccggctgc cccgccagcg cctctttcgc gttgttgaac agattcgtca
      601 gcacctgccg cagctcgtcg ggatcggctt cgagcgtcag ccccggctcg cactccgcct
      661 ccagggcgat tccgatcttc cgggcctcca gcccgaggaa caccacggcg tcggcgatca
      721 gccggtcgag cgcgaccttc tgccgcccct tcttctcctc gcgcgcgaga gcgaggaagc
      781 gcgtgatgat gtcgttgagc cgccgtgtct cggcccggat ctgcccggtg aacccgacaa
      841 actcctcgcg gttcgcagtc ggcgtgaatt ccgccgccag ccgctgggcc gcgatcgaaa
      901 tcgtgttgag cgggttgcgg atctcgtggg ccaccccggc tgcgaggtcg cccagctccg
      961 atagccgcgc ccgccgcagc gcccgccgct cgtactcctt caggcgcgtc acgtcgtaca
     1021 gaacgatcac catgccctcg tcgcgccgct gcccgatcgc cgaaaccgcc agcacgagcg
     1081 tcttctcccg gtcgccgatc gcggccgtca gctccttctc ctcgcccgca gccatccacc
     1141 gccgaaggtt ctcccgcgcg agcgccggcg ccgtcaccgc gtcgtcccag ttcctgccca
     1201 gcaccccggc cgcgcccacg atctgttcga aggcccggtt tgccagcgtg atcacccccc
     1261 gatcatccac ggccgcgacc ccggtgcgca tccggtcgaa tatcacgtcg gtgagcgatt
     1321 tgatgtccgt gtactgccgc cccagctctc ggcgcttccg ccgcgccgcc aggtagagca
     1381 ggccgaacag aagcaccccg aacagcgccg ccgagagcgc cgccatctgc agatcgaacc
     1441 gccgcgacac gctgtagtag cggccgagcg acagccccac ccggaacagc ccgaacgggt
     1501 actcgctggt cgcgaacggc cgcacgagct ccagcacccg ctccccctgg aactcgctgg
     1561 tccggtgcat gatcgtgtcg gcatcgagcg cctgcgtgag aaacggatcc gactcgatcg
     1621 ccagcagcgg tccggtgcgc cgcgaggaga agatgattcc gtcggtcgac tggtagatga
     1681 tgtacacaac ccccggctcc tccgccatcc gctgcaccag gtagccgatc tgcgtctggc
     1741 ggagcgcctc gacgtagtac tgcgcgtcgg cgaccagcac ggcgatccgg tcgagtgtgt
     1801 tggcgatctg gagatagtag tggcgggcgc cgtgctcccg cgtcgccggc tccagaagca
     1861 gtacgtagtt cgtctgcggg ttgtccccca acgttcggac ctcggcctgt acgaactggg
     1921 gcagcagccc ctcccaggcc ggcgccgcgc cggtcgccag cacgttcccc tcgagatcgc
     1981 acaggtagat cgcgtcgaga tcgtgcagga gagcggcctc ggtcagctca gccgctgtcc
     2041 gcggatcgag cgcaagctgc gacaccgtga cgaagacctc ttcgaaccgg aggtggacga
     2101 ggtagtcgta gaag
//
LOCUS       DAOPOE010000188         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_1582 NODE_58194_length_2076_cov_5.507669, whole genome
            shotgun sequence.
ACCESSION   DAOPOE010000188 DAOPOE010000000
VERSION     DAOPOE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854787
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:21:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,808
            CDSs (total)                      :: 2,766
            Genes (coding)                    :: 2,751
            CDSs (with protein)               :: 2,751
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 14 of 15
            Pseudo Genes (internal stop)      :: 1 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58194_length_2076_cov_5.507669"
                     /isolate="AMR_MDS_1582"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..765)
                     /locus_tag="PKM94_14035"
     CDS             complement(13..765)
                     /locus_tag="PKM94_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOD67797.1"
                     /translation="MRPLILSTLAALWIGGPSVWAAEGGLDPALVARIRADYDATGFS
                     PATVNAVTNNDLRALALSREKVTAFDDLFNVRLKETGITNQAGSGRCWLFAGLNVFSP
                     RVCTKLDLSKFQFSQPYLAFWDKMEKANFFLEEMIRLRERPLYDRELAVALDGPFGDG
                     GWWHYATALIEKYGVVPLTAMPETKQSGATGTVNSLAERKLRAFAAEIRRMAAGGADV
                     AQLRARKERMLADLPPPRRRLRPAADRVHLPL"
     gene            complement(879..>2076)
                     /locus_tag="PKM94_14040"
     CDS             complement(879..>2076)
                     /locus_tag="PKM94_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD67798.1"
                     /translation="LDPAAPGAVCTVAVSLETGWASMAGFDFLITYENGPLAVQSVIQ
                     GEVGRTCDWEYFTWHAGPGGPRNEYGRATDTVRIVSVASLTPTHVPACYNRAAKPVGL
                     FSLVFRTAPGPEYGCRYSPVRFYWRSCYDNRISVPASITPLPHNEVETIAQLVLEPTG
                     WQISRFSSPLPGWFGPPDTCLSERRSSPVPFVNLINGGVVLRGGSCAADPRGDLNLNG
                     IPYEIEDSWMFAKYLLLGDTALARRRTASLAATDMNRDARTGTIEDLVLLMRAVLGEY
                     VPDPGMFLNRDAPAESPFAPPPPKDSRATLRLEENELAVEAEDSLVAARFVFRGRIVP
                     HVYNTRLDVYSTYVNGQTRVLVYGFFSGASFGSGPFLYWEGSGRLTGFSLATVSGGRV
                     EVRIRR"
BASE COUNT          359 a          706 c          693 g          318 t
ORIGIN      
        1 gagtccttga ccttatagcg gaaggtgaac tcggtcggcg gcgggccgta ggcggcgacg
       61 aggaggcggt agatctgcca gcatccgttc cttgcgcgcc cgaagctgcg cgacatccgc
      121 gcccccggcg gccatccgcc ggatctccgc cgcgaaggcg cgcagcttgc gctcggccag
      181 cgaattgacc gtccccgtcg cgcccgactg cttcgtctcc ggcatcgccg tcagcggcac
      241 cacgccgtat ttctcgatca gcgcggtggc atagtgccac cagccgccgt ccccgaaagg
      301 accatcgagc gccaccgcca gttcccggtc atacagcggc cgctcccgca ggcggatcat
      361 ctcttcgaga aagaagttcg ccttttccat cttgtcccag aaggcgagat acggctggga
      421 gaactggaat ttcgacaggt cgagcttggt gcagacccgg ggggagaaga cattcagtcc
      481 cgcaaaaagc cagcaccggc ccgagccggc ctggttggtg atcccggtct ccttgaggcg
      541 cacgttgaac aggtcgtcga aggcggtcac tttctcccgg ctcagggcga gcgctctgag
      601 gtcgttgttg gtgacggcgt tgacggtggc cggcgaaaac ccggtcgcgt cgtagtcggc
      661 gcgaatcctg gccaccaggg cgggatccag gccgccctcc gcggcccaca ccgacgggcc
      721 tccgatccac agggcggcaa gggtactgag gatgagcggg cgcatgtgtt gtctctccct
      781 tctgagcgtc gatattcccg accgttggcc ggcgattttc ccggcaaggt agcggatttg
      841 cgcggagcgg gcaacggcga ttgtcgccgc gggcgggatt atcggcggat gcgcacctcg
      901 acccggcccc ccgacacggt cgccagcgag aaacccgtca gccggccgct cccctcccaa
      961 tagagaaacg gcccgctgcc gaaggacgcc ccgctgaaga acccgtagac gagcacgcgc
     1021 gtttgtccgt tgacataggt gctgtacaca tcaagccggg tgttgtagac gtgagggacg
     1081 atccgtcccc ggaacacgaa ccgggccgcc acgagcgagt cctcggcttc cactgcgagc
     1141 tcgttctcct cgagccgcag ggtcgcccgg ctgtctttcg gggggggcgg cgcgaacggg
     1201 ctctccgcgg gcgcgtcgcg gttgagaaac atccccgggt ccggcacata ctcgcccagc
     1261 accgcccgca tcagcagcac gagatcctcg atcgttcccg tccgcgcgtc gcggttcatg
     1321 tcggtggccg ccagcgaggc ggtccggcgc cgcgccaggg cggtgtcgcc caacaggagg
     1381 tacttcgcga acatccagga atcctcgatc tcgtaaggga tgccgttgag attgagatcc
     1441 ccccgcggat cggccgcgca ggagccgccg cgcaggacga cgccgccgtt gatcagattc
     1501 acaaacggca ccggcgagga ccgccgctcc gataggcacg tgtccggcgg accgaaccag
     1561 cccggaaggg gagaggaaaa gcgggagatc tgccacccgg tcggttcgag caccagttgc
     1621 gcgatcgttt cgacctcgtt gtgcggcaac ggggtgatcg aggcgggcac ggaaatccgg
     1681 ttgtcgtaac acgaccgcca gtagaaccgg accggcgagt agcggcagcc gtactccgga
     1741 cccggcgcgg tccggaaaac cagcgaaaac agcccgaccg gcttcgccgc acggttgtag
     1801 caggccggca cgtgggtcgg cgtgagactg gccaccgaga cgatccggac ggtgtcggtg
     1861 gcgcgcccgt attcgttgcg cggtccgccc ggcccggcgt gccacgtgaa gtactcccaa
     1921 tcgcaggtgc ggcccacctc tccctggatg accgactgaa ccgccagcgg gccgttctca
     1981 taggtaatga ggaaatcaaa cccggccatg ctggcccagc cggtctccag cgacaccgcg
     2041 accgtgcaga ccgccccggg cgccgccggg tccagc
//
